LOCUS       DATBTE010000001         4897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2117618, whole genome shotgun sequence.
ACCESSION   DATBTE010000001 DATBTE010000000
VERSION     DATBTE010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2117618"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VIG33_00005"
     CDS             complement(<1..793)
                     /locus_tag="VIG33_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8269240.1"
                     /translation="MQTILLFLSFILIANTAQAQVFKNISVLQNSLGFKKYYATSNPP
                     HPLKVAIFDKGFEGFQTEIGKSLPANTVYIPGPVPAPDDLKTEHGLRMAQIFTALTTN
                     NLQQPQMIQEFYLYNVFGFSNFKAAIDDAIQRGVDVISYSEVWEYGGNHDGHGFINAQ
                     VARATAAGILWINAAGNFALTSYNSAISTGNDDWVKLPDQNSALTLRCETNKKGKCSI
                     KAVLSWNDFKNDVDPGTNKDLDLALADDMLNIVQTSALHQSMDPSE"
     gene            complement(864..1295)
                     /locus_tag="VIG33_00010"
     CDS             complement(864..1295)
                     /locus_tag="VIG33_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269241.1"
                     /translation="MMRLIISAALIFGMGSLATAGELDNESGVTNKELQGTIVLRVNT
                     VTNETSFVKTEAAMSSEADAKAFAQAAEFAKVPEAKMKSELDHDGGASSWYFYSPYSQ
                     YNYNYYQPSCNWYGNSYNPYYQYNYGYYNYYYYGSCWSYCR"
     gene            1417..2145
                     /gene="tsaA"
                     /locus_tag="VIG33_00015"
     CDS             1417..2145
                     /gene="tsaA"
                     /locus_tag="VIG33_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEY8269242.1"
                     /translation="MMNFDPVNDLKPIGYLQSSYRDKFGTPRQSGLSPHAWSCLKIRA
                     DLQPEQALQGLEKFSHLWLVFVFHQNKTARYHAKVHPPRLGGESMGVFATRSPHRPNP
                     IGLSLVELVRIEEDTLILAGADLVDDTPILDIKPYLPEIESKPEAKTGWLEKAEKKQI
                     KIEFLPEVESLLCIWQEQHPDKKLREIIVDTLKLDPRPVLYRGYESGDSPYRSDHAFR
                     LLDGDVHFRFTSPENVMVFKILFK"
     gene            2216..3028
                     /locus_tag="VIG33_00020"
     CDS             2216..3028
                     /locus_tag="VIG33_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269243.1"
                     /translation="MNPQIPEDDSLKVKHYFQMGIAVGLVVAAVSGFIWFRSDSSTPK
                     ANAAEVAASDTPEDSGSDLHLDEELDVKVGADPTATPENKKAEAVDLNKVSEFFARSM
                     KQLATCMALNNAGTADRLDPSSESLMAAVKADLGDPVLRSQDWITWNLRVNSPAGSEE
                     RRIRIETDYSENDQAARHLLYFKLDAQGQPTLIPLPSEQSKDPSESFIASLQKDGEVY
                     QEEKGERVYFDGGQEMMLTEKNGKIDDLEVSNGVKTFRCAGIISSSPSCKCY"
     gene            complement(3045..3527)
                     /locus_tag="VIG33_00025"
     CDS             complement(3045..3527)
                     /locus_tag="VIG33_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269244.1"
                     /translation="MKALALATLFISLLSSNLSSAVNFPLSPNEQMTPGKLCSSSDNT
                     RYPERIRYCNRDVSSDLKQQIIVNYDRAFGYRIESLPRDQFKIDHYIPLCVGGANDMS
                     NLWPQHVSVYSITDSLEELVCKKMAEGKLRQKDAIKIIIEGKHHLDDVPEILHHVGAL
                     "
     gene            complement(3663..4220)
                     /locus_tag="VIG33_00030"
     CDS             complement(3663..4220)
                     /locus_tag="VIG33_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269245.1"
                     /translation="MKYLILGFLLLSSNLYASLTMAAPHPATSTSLLTDPQQGHYFQS
                     QGFNLKTLGTDWLPTPQNKDSLFESVRFTPKGSIESSASLSVRTDTLVKQTSLEIYAK
                     KWMRDYPSYGFEVLGAKSFTVAGSPGLVVDMVQKNKNKQIRQVILQKGQRVAILTCLD
                     DQTSFQKTLLSCNQLVKNFEWNKKN"
     gene            complement(4217..>4897)
                     /locus_tag="VIG33_00035"
     CDS             complement(4217..>4897)
                     /locus_tag="VIG33_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269246.1"
                     /translation="TDEIKEHPFVMDADPRYPKTEVYHSDLFGNDIDRLTHSSGFDSE
                     MIFVPLKKQVLFTSTRGGIPGVYWLDLRTNAVLPFQFNKERPQRSPSLASDGKTLFWI
                     EEDLIDKTQNIVTASVLGKNRAVVRNLKGIIHNVVFSPKNQLIYSWIPEGAEFSQVDL
                     YDETKPCTQTLLKHKLHFSEMQFSVKNPNLMLFRVADSDSSQVYRWELPVDLGPCNEG
                     SPSDTLKK"
BASE COUNT         1337 a         1015 c         1180 g         1365 t
ORIGIN      
        1 tttcgctggg atccatactt tgatgaaggg cgctggtttg gacgatgttc aacatatcat
       61 ctgccagagc taaatctaaa tctttgtttg tgccgggatc gacatcattt ttaaaatcat
      121 tccaagaaag aacggcttta atagaacatt tgcctttctt atttgtttca caacggagag
      181 tcagagcact gttttgatca ggaagtttta cccaatcgtc attgcccgta gagatggccg
      241 agttgtaact ggttaaggcg aagttccccg cggcattaat ccaaagaatt cccgccgccg
      301 tggcccgggc cacttgagca ttgatgaaac cgtggccgtc atgatttccg ccatactccc
      361 aaacctccga gtaggaaatg acatccactc cacgttgaat ggcatcatcg atcgccgcct
      421 taaaattcga gaaaccaaaa acattgtaaa gataaaattc ttgaatcatc tgtggctgtt
      481 gcagattgtt ggtggtcagg gcagtaaaaa tttgggccat tcgcaggcca tgttctgttt
      541 ttaaatcatc gggtgcgggg acagggccag gaatatatac agtgttggcg gggagggatt
      601 tgccaatctc agtttgaaag ccttcaaacc ccttatcgaa aatcgctact ttcaggggat
      661 gaggagggtt cgaggttgca taatactttt taaaacccag ggaattttga agaaccgaga
      721 tatttttaaa aacttgggcc tgagccgtgt ttgcaattaa aatgaaagag agaaagagca
      781 gaatggtttg cacgacatac ttcctttttc agaatcaggg caaaaaaaaa gctggttcaa
      841 agaaccagcc ttatacagca gagttatctg caatacgacc aacaagagcc atagtagtaa
      901 taattatagt acccgtagtt gtactgataa taggggttgt aggagttgcc gtaccagtta
      961 caagaaggct ggtagtaatt gtagttgtac tgggaatagg gagaatagaa ataccagcta
     1021 gaagcgcctc catcatgatc aagttccgac ttcatttttg cttctggaac ttttgcgaac
     1081 tccgcagctt gtgcaaaagc tttggcatcc gcttcagaac tcatagccgc ttctgtctta
     1141 acgaaagagg tctcattggt cacagtgttt actcgtaaaa cgatcgtgcc ttgaagttct
     1201 ttgttagtaa ctccggactc attatccagt tcgccggcgg tcgccaaaga gcccatgcca
     1261 aagatcaaag ctgcagaaat aatcaaacgc atcataaatg cctccttttt aagttgttta
     1321 atctgcgcac taaataatca gtgtctggac gcaagtcatc agaaatgtgc ttgtcttttc
     1381 cttatctttt ttgtgaagac tgtgcgaaga gtggcgatga tgaattttga ccctgtaaac
     1441 gatttaaaac ccataggtta cctgcaatcc tcctatcggg ataaatttgg aacgccgcga
     1501 caatctggcc tttctcctca tgcttggtcc tgtcttaaaa ttcgtgcgga tttgcagcct
     1561 gagcaagcgt tgcaagggct cgaaaaattc agccatttat ggcttgtctt tgtctttcat
     1621 caaaataaaa cggctcgtta tcatgccaaa gttcaccctc cccgtttggg cggagaaagt
     1681 atgggagtct ttgcaactcg aagtccccat cgtcctaatc ctatcgggct ttccctggtc
     1741 gaattggttc gcattgaaga ggacactctt attcttgctg gagcagacct ggtggatgac
     1801 acacctattc tagatataaa accttacctt ccagagattg aatccaagcc tgaagcgaaa
     1861 acaggatggc tcgaaaaggc ggagaaaaag caaatcaaga ttgagttttt gccagaagtc
     1921 gaatcgctcc tatgcatttg gcaagagcaa catcctgata agaaactgcg tgagattatt
     1981 gtggatactt tgaagttaga tcctcgtccc gtgctctatc gcggctatga gagcggggac
     2041 tccccttatc gcagcgatca cgcttttcga ttgctggatg gagatgtcca tttccgcttt
     2101 accagtccag aaaatgtcat ggtttttaaa atccttttca agtaaccttg aggtatatct
     2161 gtgtctctct ttgtgtcctg gcgaggaaag ttgggaaaac taaaaaggct ttagaatgaa
     2221 tccgcaaatt ccagaagacg acagtttaaa ggtaaaacat tattttcaga tgggtattgc
     2281 cgtgggtctt gtggtggctg cggtgagcgg cttcatttgg tttcgtagcg attcctctac
     2341 gccaaaagcg aatgcagcgg aagtggcggc ttcggatact cctgaggact ctgggtcgga
     2401 tttgcactta gacgaggagt tagacgtcaa ggtgggagct gatcccacag cgactccgga
     2461 aaataaaaaa gcagaggccg tcgatctgaa caaggtttct gaattttttg cccgtagcat
     2521 gaagcaactt gcgacttgta tggctcttaa taatgcggga actgcggacc ggcttgaccc
     2581 aagcagtgag agtttgatgg cagccgtaaa agctgatttg ggggatccgg ttttacgctc
     2641 tcaagactgg atcacttgga atttgcgggt gaattcccct gcgggaagtg aagaaagacg
     2701 cattcgcatc gaaactgatt attcggaaaa cgatcaggcc gctcgtcatt tgctctattt
     2761 taagctcgat gcccaagggc agccgacctt gatccctttg cccagcgaac aaagtaaaga
     2821 ccctagtgag tctttcattg cgtccttaca aaaagatgga gaggtttacc aagaagaaaa
     2881 aggggaacgt gtttacttcg atgggggaca agagatgatg ctcacggaga aaaacggcaa
     2941 gattgacgat cttgaagtgt ccaatggagt taagaccttt cgctgtgcgg ggatcatcag
     3001 ctcgtcccca agctgcaagt gttattgata gaaaccttcc tctatcacag agcccctaca
     3061 tggtggagga tttctggaac atcgtcgaga tgatgcttgc cctcaataat aattttaatg
     3121 gcgtcttttt gccgaagttt tccttcagcc atttttttgc aaactaattc ctctaatgaa
     3181 tccgtgatgc tgtagacgct tacatgttgt ggccacaggt tgctcatgtc gttggcgccg
     3241 ccgacacaaa gagggatgta atggtctatc ttgaactgat cgcggggcaa agattcgatt
     3301 cggtaaccaa atgcacggtc gtagtttacg atgatttgtt gcttgaggtc tgatgacacg
     3361 tcccgattgc agtaacgtat tctttctggg taacgagtat tgtcggaaga agagcagagt
     3421 ttgcctgggg tcatttgctc atttggactc agaggaaagt taacggcgga agagaggtta
     3481 gaacttaaaa ggctgatgaa gagggtcgcg agagcaagcg ctttcattta atctccaagt
     3541 aatctgtctt ttacggaagc tactcagcat cttatagttt tattttgtga tgaagagcaa
     3601 aggatttaaa aaaaagaatc ttattttcaa gaggggatgt aaaaattgct atccaggaag
     3661 acttagttct ttttattcca ctcaaagttt ttaacgagtt gattgcaaga gagaagggtt
     3721 ttttgaaaac ttgtctggtc atccaaacag gtcaggatgg ccaccctctg tcccttttgc
     3781 aaaatgactt gacggatttg tttgttttta tttttttgca ccatatcgac gacgagtcca
     3841 ggactgccgg caacagtaaa ggatttggct cctaaaactt caaagccata gctgggataa
     3901 tcacgcatcc atttttttgc ataaatctct agactggttt gtttcactaa agtatccgtt
     3961 cgcaccgata atgaagccga agattcaata cttcctttgg gagtgaaacg aaccgactca
     4021 aacaaggaat ccttgttttg cggagtgggc aaccaatccg tgccaagtgt ttttaaatta
     4081 aatccctgac tttgaaaata atggccttgt tgaggatctg tcagtagaga ggtactggtg
     4141 gcaggatgag gagccgccat agtaagagat gcataaagat tcgaagataa gagtaaaaat
     4201 cctaagatta agtatttcac ttttttagtg tatctgaggg gcttccttca ttgcaagggc
     4261 caagatctac aggcaattcc caacggtaga cttggctgct atctgaatcc gccacgcgga
     4321 agagcattag attcggattt ttcacagaaa actgcatttc ggaaaaatga agtttatgtt
     4381 ttaaaagagt ctgagtgcat ggtttggttt catcgtaaag gtccacttga ctgaattctg
     4441 ctccttcggg aatccagcta taaataagct ggttcttagg actgaaaacc acgttgtgga
     4501 taatcccttt taggtttcgt accaccgctc gatttttacc caagaccgag gcggtcacta
     4561 tattctgggt tttgtcgatc aggtcctctt caatccaaaa gagggttttg ccatcagagg
     4621 ccagcgaggg actgcgttgg gggcgctctt tattgaactg gaaagggagc actgcatttg
     4681 tacgaaggtc cagccaataa acgcctggaa tcccgccacg ggtcgaggtg aaaagcacct
     4741 gcttcttcaa gggaacaaag atcatctcac tatcgaagcc ggaggaatga gtcaggcggt
     4801 caatgtcgtt gccaaataaa tcactatgat agacttcggt tttgggatac cgcgggtctg
     4861 catccatcac gaagggatgt tccttaattt catcggt
//
LOCUS       DATBTE010000002         5210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2186030, whole genome shotgun sequence.
ACCESSION   DATBTE010000002 DATBTE010000000
VERSION     DATBTE010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2186030"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..667
                     /locus_tag="VIG33_00040"
     CDS             113..667
                     /locus_tag="VIG33_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269247.1"
                     /translation="MKDLLKNLKSEHEFILNEMRILAEALSINQKRLHDLFQFVEFTH
                     HEKEENILFPWIARQNWLRHGGPLCGHFMGIRLDLNPLARINQHLDDFNRMSLAKKDS
                     ILLPPDKKFAWLSPQSPLSIPMEEHEVGHRLSEALRHLMQISAEASQEAIFRTLLQDY
                     FSLLKMHIHKEDHCLFVQCEQSLF"
     gene            complement(675..935)
                     /locus_tag="VIG33_00045"
     CDS             complement(675..935)
                     /locus_tag="VIG33_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEY8269248.1"
                     /translation="MQEFVIEAQKIPAQDRHPFIFQSFDNLQGGDSLIIINTHDPNPL
                     LRQLQEDRPEQFSHEYLLQGPKEWRVRISKIKKEGCCGFCGS"
     gene            1123..1635
                     /locus_tag="VIG33_00050"
     CDS             1123..1635
                     /locus_tag="VIG33_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY8269249.1"
                     /translation="MTKDANKRDSNPKIKHLTFRLGDSEYGVPLSVVKEVIGLPLLVE
                     IPGGPNYFLGLINLRGKVITAIDLRTKLGFEPEVSRKKRQAIVVTDVGEIILGCVVDS
                     ISAVINISEADIDRDLSINIPGNRNAIQGVAHFKDRPMILLLDLKQAADISEVTELKS
                     KELHKEGRVS"
     gene            1632..3299
                     /locus_tag="VIG33_00055"
     CDS             1632..3299
                     /locus_tag="VIG33_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8269250.1"
                     /translation="MKNHRTFGIKAKLSILVGIAVVLICGQGFFSYTVSRSLVESVEH
                     LGNQRLPLSTTMSDMRSAINSAPRFMWLALAYDADNAERKKFLDKAAESMDLLAKSTE
                     LYGTFQITDEARVKLESIKEPIAKLNEELSKATVLLEAGKNDAEAKQFLLKNMPPIAV
                     KATSVIDDLAALAVTRNKAVVLEAQSNAKQAQIITAFFSILCSLGLAAFGYIFSSQLA
                     KRLMSITNTVGEASSQVASASTQLSNAADSLSSSSQEQASSVQETSSSLTEIAGMVES
                     NVKVAESANHVAQEVFEISEQTQESMEHLVDAMSLILESNTRIEALVKVIEEIGERTE
                     IIDDIVFKTQLLSFNASVEAERAGEHGRGFAVVAQEVGNLAQMSGKAASEISSIVKTS
                     IKEAECVAKDNKSRVEKGSSLAVETKQKMIESLERIKQILEGTQKIVSASKEQSQGVN
                     QISTSVDSLNQTVQETASTAEESASASQELSSQAESLMALVNELRLIVSGSSATTSEA
                     ANKMDSRSQTNIIPFKQKTSSNSPPLKSAPRRQPPLKATASDPWDAL"
     gene            3328..4185
                     /locus_tag="VIG33_00060"
     CDS             3328..4185
                     /locus_tag="VIG33_00060"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEY8269251.1"
                     /translation="MNSNEQRKLIFNENEYEILSELFHDETGIILNRSRESLIESRLR
                     KRLFELQLVGSDYIRLVSKDASERKRFVDFLTTHKTEWFREPVHFNFLNEWLHTEGTH
                     IAQKRPLMFWSAASSTGEEAYSIVLDILKNNFSPTQFRILGTDISTDCVDKAELGTFP
                     YSGISNQRTCDILRRGFTKSLGPKGERQIQARADVQASIKFRIFNLYADSLPDDLLFD
                     VIFLRNVLIYFSAEKSAAIIQKACKHLRIGGYLVLGLSESLPPDDEFKSFFKNIKSQG
                     SSIYRKINP"
     gene            4299..>5210
                     /locus_tag="VIG33_00065"
     CDS             4299..>5210
                     /locus_tag="VIG33_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8269252.1"
                     /translation="MAAVSGDNKFFFSKEISKLLKTMKALGSERKDIEIVICGSKQII
                     AYLQLKEFFLSFEIESVKFLFVNSDKNVTLDFTPFNFQCRIPETEINSVFPALLEPQR
                     KIKVLIVDDSFVIQKILNSVLSTDAMIEVVGMAHDAFQAEEIRKKTHPDVMTLDIHMP
                     KKDGVTYLAELLSADAIPVIMISDLSRNEASPVMRALEMGAFDYLQKPSAQDVKDFGE
                     ALIGLIKVAGGRKPHLARSVKRKKERKRPSSLPYSARASQGSLVAIGSSTGGTEVIRE
                     IISSFPEDMPPTLIVQHMPAVFTAKFAD"
BASE COUNT         1587 a         1123 c         1113 g         1387 t
ORIGIN      
        1 aaaaaaggct ggattctgac ccggtcaaaa cacgtggaaa tcttgaatcg gcaagccctg
       61 gaaaatttaa aaacgtcttc aagtaaaact tagagcgcat gaaggctttt atatgaaaga
      121 tcttttaaaa aatcttaaat ccgaacatga attcattctt aacgaaatga ggatcctcgc
      181 ggaggcactc tctatcaatc agaaacggct gcatgattta tttcaatttg ttgagttcac
      241 tcaccacgaa aaagaagaaa atattttatt cccgtggata gcccgacaga attggcttag
      301 acacggcggt cctctctgcg gacactttat gggtatacgt ctggatctga atcccctggc
      361 gcgaataaat caacatttag atgatttcaa tcgaatgtct ctggctaaaa aagattcgat
      421 tcttcttccc ccagataaga agtttgcgtg gctcagcccg caaagcccct tgagtatccc
      481 gatggaagaa cacgaggtgg gacatcgcct gagcgaagct ctgcggcatt taatgcaaat
      541 ctcggcagaa gcttcgcaag aagcgatttt tcgcactctt cttcaagatt atttttcact
      601 gctcaaaatg catatccaca aagaggacca ttgcctcttt gtgcaatgcg aacaaagtct
      661 attctaagaa cgttctagga accacaaaac ccacaacaac cttctttttt gatttttgaa
      721 atacgaactc tccattcctt tggcccctgc agcaagtatt catgagaaaa ttgctcagga
      781 cgatcttctt gcaattgcct taataaagga tttggatcat gggtattgat gataatcaga
      841 gaatctcctc cttgaagatt gtcaaaactt tgaaaaataa aaggatgtct atcctgcgcc
      901 ggaatctttt gtgcttcgat cacaaactct tgcattaagc tctccttgtt tatcttattc
      961 aaatttaaag gagtctcgtt ttgaattcat tgattcaaat catttttctt acaaggggtg
     1021 accagaggac tgatccacgt catgcaacta aggataaggc ctaaagtcga gcaagcctct
     1081 caccgatcac tgttatacgt atttggaggc cttgtgaacg acatgactaa agatgcaaat
     1141 aagcgggatt caaatccaaa aataaaacat ctcacttttc gcctgggaga ctctgagtat
     1201 ggcgttcctc tgtcggtggt aaaggaagtc atcggactcc cgctgttagt ggaaatacct
     1261 ggaggtccaa attattttct aggacttatt aatttgcgcg gaaaagtgat cacagccatt
     1321 gatttgcgta caaagcttgg ctttgaacct gaagtgagcc gaaagaaaag acaagccatt
     1381 gtggttacgg atgtgggaga aattatccta ggctgcgttg tagactcgat tagtgccgtt
     1441 atcaatatat cagaagctga tattgatcgc gacctgagta taaatattcc tggaaatcga
     1501 aatgcgattc aaggcgtcgc ccattttaaa gatagaccca tgatcttgct gttagatcta
     1561 aagcaggcgg cggatatttc tgaagttacc gagctaaaat ccaaggaatt acataaagaa
     1621 ggaagggttt catgaagaac cacaggacat tcggaataaa agcgaagttg tcaattttag
     1681 tcggcatagc ggtcgttctt atttgtggcc aaggattctt ttcttacact gtttctaggt
     1741 ctcttgttga gagcgtggaa catctgggaa atcaaagact gcctctttca acaaccatga
     1801 gcgatatgcg ctccgccatc aattcagccc cacgctttat gtggcttgcc ctggcctatg
     1861 atgcagacaa cgccgagcga aaaaaattct tggataaggc cgctgaaagt atggatttgt
     1921 tggctaaaag cactgagttg tacggtactt ttcaaattac ggatgaagca cgtgtaaagt
     1981 tagaatctat taaagaaccc attgctaaac tcaatgagga actcagcaag gccactgtct
     2041 tactagaggc tggaaaaaat gatgccgagg caaaacagtt tttgctaaaa aatatgccac
     2101 ccatcgctgt aaaagcaact tccgtgattg acgatctcgc ggccttggca gttacaagaa
     2161 ataaagcagt ggtgctcgaa gcacaatcaa atgcaaagca agctcaaatc atcacggctt
     2221 tcttttcaat tttgtgttcc ttgggattgg ccgcttttgg gtatattttt tccagccaat
     2281 tagcaaaacg tctgatgagt attacaaaca ccgtaggcga agccagctcc caagtggctt
     2341 ccgcgagcac gcaactttca aatgccgctg attccttaag ctccagctct caagaacagg
     2401 ccagctccgt tcaagagact tcttcttctt tgactgaaat cgctggcatg gtggaatcga
     2461 atgtcaaagt cgcggagtcg gcaaaccatg tggctcaaga ggtctttgaa atcagtgagc
     2521 aaacccaaga gtccatggag catttagttg acgcgatgag cttgatcctc gaatccaata
     2581 cgcggattga agccttagtg aaagtgattg aggaaatcgg cgagcgcact gaaattatag
     2641 atgatattgt gtttaaaact caacttctct cattcaatgc ttccgtcgaa gctgaaagag
     2701 cgggcgagca cgggagagga ttcgccgtgg tcgcacaaga ggtcgggaac ctcgcgcaaa
     2761 tgagtggcaa ggcggcttct gaaatttctt cgatcgtgaa gaccagcatc aaagaagccg
     2821 aatgcgtcgc caaagacaat aaatcacgtg ttgaaaaagg cagttccctc gctgtcgaaa
     2881 caaaacaaaa aatgatcgag tcgctagagc ggatcaaaca aattttagaa ggcacacaaa
     2941 aaattgtttc agcaagcaaa gaacaaagcc agggtgtcaa tcaaatcagc accagtgttg
     3001 acagtttaaa tcaaaccgtc caggaaacag caagcacagc tgaagagtcc gccagtgcca
     3061 gccaagagct ttcatcacaa gcggaatcct tgatggcttt ggtcaatgaa ctcagactga
     3121 ttgtcagcgg tagttcggcg acaacctcgg aagcagcaaa taaaatggac tcaaggtctc
     3181 aaaccaatat catccccttt aaacaaaaaa cctctagtaa ctcaccgcca ttgaagtcag
     3241 caccaagacg tcaacctcct ttaaaggcaa ctgcttccga cccatgggac gctttataat
     3301 aaaagttcca aaaaaggagt gactgtcttg aactcaaatg agcagcgtaa attaatattt
     3361 aatgagaacg aatatgagat tctctctgaa ctgtttcatg acgagaccgg aatcattctg
     3421 aatcgatccc gtgaaagttt gattgaatcc cgtttaagaa aacgtctttt tgaactgcaa
     3481 ttagtggggt ctgactatat cagattagta tccaaggatg ccagtgaaag aaaacgattt
     3541 gtggatttct tgacgacgca taaaacggaa tggtttcgag agccagttca ttttaatttt
     3601 ttaaatgaat ggttgcatac ggaggggact cacattgcgc aaaagcgacc cttgatgttt
     3661 tggtccgccg cttcctctac aggggaagaa gcttactcca ttgttcttga tattttgaaa
     3721 aataatttca gccctacaca gtttcgtatc ctgggaaccg acattagtac ggattgcgtg
     3781 gacaaagcgg agctcggaac ctttccgtat tcaggtattt caaatcagag gacttgtgac
     3841 atcttaagac gcggttttac caaatcctta gggccaaaag gcgaaagaca aattcaagcc
     3901 cgtgccgacg ttcaagcttc aattaaattt cggatattta atttatacgc ggactcctta
     3961 cccgacgacc tcttatttga tgtgattttt ctcagaaacg ttcttattta tttttcagcg
     4021 gaaaaatcag ccgctatcat acaaaaagcc tgcaagcacc tcagaatcgg tggatattta
     4081 gttctgggac tttccgaaag tttaccgccg gatgatgagt tcaagtcctt ttttaaaaat
     4141 attaaatctc aaggttcttc catctatcga aagataaatc catgaacttc caaagtattt
     4201 cacctgaagt ccaattattt acgaacagaa gcgcccctca cagctttcac attttcaact
     4261 ccgccgtcgt gctccttaaa acgaaaagag gagaaagaat ggcggcggtc tccggtgaca
     4321 ataagttctt ttttagcaaa gaaatatcaa aacttctcaa aaccatgaaa gcgttgggtt
     4381 ccgaaaggaa agatattgaa atagttattt gcggcagcaa gcaaataatt gcttatttgc
     4441 aacttaaaga attctttctc tcctttgaaa tagagtccgt gaagtttcta tttgtgaata
     4501 gcgataaaaa cgtaacgctg gattttactc cttttaactt tcagtgtcgc attccagaaa
     4561 cagagataaa ctctgttttt ccggcattac tggaacctca aagaaaaata aaagttctta
     4621 tcgtagacga ctccttcgtg attcaaaaaa ttcttaactc cgtcttgtcc acggatgcta
     4681 tgattgaagt cgtaggcatg gcccatgatg cttttcaggc ggaggaaata agaaaaaaaa
     4741 ctcatccgga tgttatgact ttagatattc atatgccaaa aaaagacgga gtgacctatc
     4801 tcgcagaact cttatctgca gatgcgattc cggtcatcat gatcagtgat ttatcacgaa
     4861 acgaagcctc tccggtgatg agggcgcttg agatgggagc ttttgactat ttgcaaaaac
     4921 catcggcgca agacgttaag gattttggcg aagcactcat tggcctgata aaagtcgcag
     4981 gcggcagaaa acctcacctc gcccggagcg taaaaagaaa gaaagagcgc aagcgacctt
     5041 cgtccctccc ctattcagcc agagcttccc agggctcact cgtagcaatc ggctcttcaa
     5101 ctggaggaac cgaagtgatt cgtgagatta tttcaagttt tcccgaagat atgccgccga
     5161 ctctcattgt tcaacatatg ccagccgtat ttacagctaa atttgccgac
//
LOCUS       DATBTE010000003         5996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1776228, whole genome shotgun sequence.
ACCESSION   DATBTE010000003 DATBTE010000000
VERSION     DATBTE010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776228"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..3707
                     /gene="metH"
                     /locus_tag="VIG33_00070"
     CDS             102..3707
                     /gene="metH"
                     /locus_tag="VIG33_00070"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEY8269253.1"
                     /translation="MKKSALLLELEQTLKKRLMFIDGAMGTMVQLYKLSEADYRGERF
                     KDHPKDLKGNNDLLVLTQPKVIYDIHMAYLEAGADIIETSTFNGTSIAQTDYGLESLA
                     FELNKEAAALAKKACVDFEKKTGKKCYVAGAIGPTNKTASLSPDVNNPGYRAITFDEL
                     KAAYKEQTLGLLAGGADILLPETIFDTLNVKAALFAISEIEEERGEKLPLMISVTITD
                     LSGRTLSGQTVEAFWNSVRHSQPLSVGINCALGAKEMHPYIRELARVADCAISCYPNA
                     GLPNPLSLTGYDETPESLAFQLGLMAEEGIVNILGGCCGTTPNHIRKIAESLAPIKVS
                     PRSERSFEAMRLSGLEPFNFANLQAGQARSFVMVGERTNVSGSPKFAKLIKEDKYDEA
                     VEVARSQVTSGANILDVNFDEGLIDGAKAMTRYLNLISAEPDIARVPIMVDSSKWEVL
                     EAGLKCLQGKAIVNSISLKEGEEAFLKQARSIQRYGAAVVVMAFDEKGQAASKEDKVR
                     ICERAYHLLAEELDFDPADIIFDPNILTVATGMEEHNNYAVDFIEAVREIREKCPGVS
                     FSGGVSNLSFSFRGNNKVREAMHAVFLYHAIRAGLDMGIVNAGMLEVYEEIPVHLREL
                     VEAVVLNKHAHAAEDLLNEAEKFKAEKGSEKAVDTGLEWRKHSLQDRITHALVKGIDS
                     HIVEDAEEARQQFGRPLNVIEGPLMTGMKVVGGLFGEGKMFLPQVVKSARVMKKAVAY
                     LEPFMEEEKKTKTGLQSQGTLVIATVKGDVHDIGKNIVSVVMACNGYKIIDLGVMVPV
                     QNIIKAAQEEKADLIGLSGLITPSLEEMAFNLGEFQRAGFKIPILIGGATTSKVHTAV
                     KLDPHYEGVVAHVNDASLVVEVGNKLLSPNTREAYALEVKKTNATLRESYLKNQNESE
                     KVSVVEAREKAFRCDWTQIDRPLPSRTGVFELPVSIQDVRKYIDWSPFFWAWEMKGTY
                     PKILTSEKYGEEAQKIFNDAQGLLDQMIKENRIHPKILMGIFAANAKDENVFVYSEKG
                     EAVETLHFDRQTRKKVVNNDVNYSLADFIAPKELALKDHLGLFAVTAGPGVELMAKEF
                     EEALDDYSSILVKAIGDRLAEALAEYAHKNVREIFGIQEDLSAEDLIAEKYRSIRPAP
                     GYPACPDHSEKKKIWSLLGVEKRIGVRLTENYAMTPPASVCGYYFMHPNAKYFAV"
     gene            3742..4038
                     /locus_tag="VIG33_00075"
     CDS             3742..4038
                     /locus_tag="VIG33_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HEY8269254.1"
                     /translation="MDKTRNTKKNQAQEPQAPVEIPQEALSTEALRGIVESFILREGT
                     DYGREEVGFEKKVEQIERQLAKGEIKIVFDPGSESVGLVTLSEWKKLQSFHDSP"
     gene            complement(3947..4699)
                     /locus_tag="VIG33_00080"
     CDS             complement(3947..4699)
                     /locus_tag="VIG33_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269255.1"
                     /translation="MKHAIYFKIQHHPLTLSEKEVAKALELARFDPRLMELMAEHIRD
                     FWWTLEPEDLNRASRKMKSPFVIKALTSVILDRCTMTKEDHADFSQWVWFATRGIKDP
                     PPQFLFIGLTPVGSKSAFNQIKEALPSFFRHNLIAKDLPFNKGNPGSLKTENSPPANR
                     VDELDLLKFNLGKKIKKIKSERAISNHEMTKLTGINTVFLSKILNNKLENISVEYLRD
                     HLLKANHEKTEASSTHSGSQDRRFLNRDRRRS"
     gene            complement(4696..5220)
                     /locus_tag="VIG33_00085"
     CDS             complement(4696..5220)
                     /locus_tag="VIG33_00085"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HEY8269256.1"
                     /translation="MNKALALKALQTLDEKLATSTLPKVTLTVGGGGAMILKHDYSGA
                     TVDIDAVPSNIEFEELKPFMEEVAKELKIAPDWLNPYYQAFTVYLPKDSKNRMSDIYI
                     GKKLLVKSLGPEEVLIMKLMAGRAKDMTHIKHLLKMKIDLSIVEKRLEELVALFPKIA
                     EKALDLLDDLTENT"
     gene            complement(5379..>5996)
                     /locus_tag="VIG33_00090"
     CDS             complement(5379..>5996)
                     /locus_tag="VIG33_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8269257.1"
                     /translation="DITEGIPLANELADLIICQEGIEHFPDQLAALKEFNRVMKDQGT
                     LIITTPNHSNLKHKLSYLLTESERYNRIMAVNEYDSIWFNRDHNDNQYYGHVFLIGIT
                     KMRLLARIAGFKIRKIHFSEVKPSNLILFLIFYPFILLSSLMTYLRSVKKKPIAKAIY
                     LEILKLNIDPRILIDGSLIVEFEKEFSVAEAKKALHQIGSYDLQT"
BASE COUNT         1673 a         1173 c         1519 g         1631 t
ORIGIN      
        1 aagtggttaa aaagtttaaa tagtttaata attaaaattt aatagctaaa tggttttcat
       61 tttaaataat ctcatgcaaa gagactgaaa gataaactgt gatgaaaaaa agcgcgctgt
      121 tgttagaatt ggaacaaaca cttaaaaaac gtctgatgtt tatcgatgga gccatgggga
      181 ccatggttca gttgtataaa ctgagcgaag ccgattatcg aggtgaacgt tttaaagatc
      241 atcctaagga tttaaaaggg aataatgatc tcttggtttt gactcagccg aaggtgatct
      301 acgacattca tatggcctat ctcgaagccg gtgcggatat tattgagacc agcaccttca
      361 acggtacttc cattgcgcag acagattatg gtttggagtc cttggctttt gaattaaata
      421 aagaagccgc agcgctggct aaaaaagcct gcgtggattt tgaaaagaaa accgggaaaa
      481 aatgctatgt cgctggagcg atcgggccca cgaacaagac ggcttcactg agtccagatg
      541 tgaacaatcc tggctatcgc gccattactt ttgatgaact caaggccgcg tataaagagc
      601 agactttggg actcttggcg gggggcgcgg atattctttt gccggaaact atctttgata
      661 ccttgaacgt gaaagcggct ctttttgcga tttctgaaat cgaagaagaa agaggcgaaa
      721 aacttccgct gatgatctcc gtgacgatca cggatctttc tggacgcact ctttcgggcc
      781 agacagtgga agccttctgg aactcggttc gtcattcgca gcctttgagt gtagggatta
      841 actgcgcttt gggtgcgaag gaaatgcatc cctacattcg tgaattggcc cgtgtcgcgg
      901 attgcgcaat ttcttgttat cctaatgcgg gtctgccgaa tcctttaagc cttacgggtt
      961 atgatgagac tccagaatct ttggcctttc agttgggact gatggcggaa gagggcatcg
     1021 tgaatatttt gggaggctgt tgcggaacca cgccaaatca tattcgtaaa attgccgaga
     1081 gcttggctcc tattaaagtt agtccacgtt cggaaagaag cttcgaagcc atgcgtttga
     1141 gtgggcttga gccttttaac tttgccaatt tgcaggcggg gcaagcaaga agttttgtga
     1201 tggtcggtga aagaaccaat gtttccggtt cccccaaatt cgcgaagctg atcaaagaag
     1261 acaaatatga cgaagcggtg gaagtcgcaa gaagccaggt caccagtggc gccaacattt
     1321 tagatgtcaa ttttgatgaa gggttgatag atggcgccaa ggcgatgact cgttatttga
     1381 acttgatttc agcggagccc gatattgcgc gggtgccgat tatggtggat tcttcgaaat
     1441 gggaagtcct tgaagcggga ctaaaatgtt tgcaaggaaa agccattgta aattcaattt
     1501 ccctgaaaga gggcgaggaa gccttcctga aacaggcgcg cagcattcag cgatatgggg
     1561 cggccgtggt agtgatggcc tttgatgaaa aaggtcaagc cgccagtaaa gaagataaag
     1621 ttcgaatttg tgaacgcgct taccatttgt tggcggagga attggatttt gatccggcgg
     1681 atattatttt tgatcctaat atcctcactg tggcgacagg catggaggag cataataact
     1741 atgccgtgga ttttattgag gccgtccggg aaatccgcga gaaatgcccg ggagtttctt
     1801 tttctggtgg tgtctcgaat ttgagtttta gtttccgtgg caataataaa gttcgtgaag
     1861 ccatgcatgc ggtattcttg tatcacgcca ttcgtgcggg tttagatatg ggaattgtca
     1921 acgccggtat gctcgaggtt tatgaagaga tcccggtgca tctcagggaa ttggtggagg
     1981 ccgtggtttt aaataaacat gctcacgccg ctgaagatct tttaaatgaa gccgaaaaat
     2041 tcaaggcaga aaaaggttct gaaaaagctg tcgatacagg acttgaatgg cgtaagcact
     2101 ccctgcaaga cagaatcacc catgcgctgg tgaaaggcat tgattctcat attgttgaag
     2161 atgcggaaga agcccgccaa caattcggtc gaccgttgaa cgtgatcgaa ggtccgttga
     2221 tgacagggat gaaagtggtc ggtggcttat tcggtgaagg gaaaatgttc ttaccccaag
     2281 tggtgaaaag cgcgcgggtg atgaaaaagg ccgtggctta cctggagcct tttatggaag
     2341 aagagaaaaa gaccaagacc ggtttgcaaa gccaagggac tttggtgatt gcgacggtca
     2401 aaggagatgt tcacgatatt ggtaaaaata tcgtctccgt agtgatggcc tgcaatggct
     2461 acaaaatcat cgatttggga gtgatggtcc cggtgcaaaa tatcataaag gccgctcaag
     2521 aagaaaaggc agatttaata ggacttagtg gcttgatcac gccttcacta gaagagatgg
     2581 cctttaattt aggtgaattc cagagagctg gatttaaaat ccctattctc attggcggtg
     2641 ccacgaccag caaagttcac accgcggtga agttagaccc tcactatgag ggtgttgtgg
     2701 cccacgtgaa tgatgcctct ttggtggtcg aggtgggcaa taaactcctt agccccaaca
     2761 ctcgagaagc ctatgcatta gaggtgaaga aaaccaatgc gactttgcgc gaaagctact
     2821 tgaaaaacca aaatgagagt gaaaaagtct ccgtggttga agcccgggaa aaagccttcc
     2881 gctgcgattg gactcagatc gatcgtccgc tgccttcccg cacaggcgtg tttgagcttc
     2941 cggtttcaat tcaggatgtt cgcaaataca ttgattggag cccgttcttt tgggcgtggg
     3001 agatgaaggg aacttatcct aaaatactga cgtcagaaaa atatggcgag gaagcgcaga
     3061 agattttcaa tgatgctcaa gggctcttgg atcagatgat taaggagaat cgcattcatc
     3121 cgaaaatact gatgggtatc tttgctgcca atgcaaaaga cgaaaatgtt ttcgtatact
     3181 ccgaaaaagg tgaggcggtg gaaaccctgc attttgatcg ccagacgcga aaaaaggtcg
     3241 tgaacaatga tgtcaattac agcttggcgg attttattgc gccgaaggag ttggcgctga
     3301 aagatcactt aggtcttttt gctgtgacgg cgggccctgg ggttgagttg atggctaaag
     3361 aattcgagga agccctggat gattattctt cgatcttggt gaaggccatc ggtgaccgtt
     3421 tggcggaagc cttggcggag tatgctcata aaaacgttcg cgaaattttt ggcattcaag
     3481 aagacctttc ggcggaggac ctgattgctg agaagtaccg aagcattcgt cccgcgccgg
     3541 gatatccggc ttgccctgat cactcagaaa agaagaaaat ctggagcttg ctcggcgttg
     3601 aaaaaagaat tggcgttcgc ttgacagaga actacgcgat gactccgcca gccagtgtgt
     3661 gcggatatta tttcatgcat ccaaatgcaa aatatttcgc cgtttaaggc gcggtttaag
     3721 ggggaggagc ttaagatggg gatggataag acgaggaata ctaaaaaaaa ccaggcacag
     3781 gagccacaag ctcctgtgga gattccccaa gaggccctca gtacggaagc gcttcgtggc
     3841 atcgtcgaga gcttcatttt gcgcgaaggt actgattacg ggcgagaaga agtgggcttt
     3901 gaaaaaaaag tcgaacaaat tgagcgtcag ttagccaagg gcgaaattaa gatcgtcttc
     3961 gatcccggtt cagaaagcgt cggtcttgtg accctgagtg agtggaagaa gcttcagtct
     4021 tttcatgatt cgccttgagc agatggtcgc gcaggtattc cacggagata ttttcaagtt
     4081 tattgtttag aatcttagag agaaacacag tgtttatccc agtgagcttt gtcatttcat
     4141 ggttggagat ggctcgctca gattttattt tttttatttt tttacccagg ttaaacttca
     4201 agagatccag ctcatccact ctgtttgcag ggggagagtt ttccgtcttt aagcttcccg
     4261 gatttccctt gttaaaagga agatctttgg caatgagatt gtgtctaaaa aatgagggaa
     4321 gagcttcctt gatttgatta aaagcagatt ttgatcccac aggagtaagg ccaataaaca
     4381 gaaattgcgg agggggatct ttaattcccc gggttgcgaa ccaaacccat tgactgaaat
     4441 cagcgtgatc ctcttttgtc attgtgcacc tatctaggat gaccgaagtg agagctttta
     4501 ttacaaaagg cgacttcatt ttacgggaag ccctgttgag atcctcgggc tctagggtcc
     4561 accaaaaatc acgaatatgt tcagccataa gttccataag tcggggatcg aagcgagcca
     4621 gttccagagc tttggcaact tccttttccg aaagcgtgag aggatgatgt tggattttaa
     4681 agtaaatcgc gtgcttcaag tgttctccgt aaggtcatct aagagatcca aagccttctc
     4741 tgcaattttc gggaatagag cgacgagttc ttcaagacgc ttttccacaa tagaaagatc
     4801 aatcttcatt tttagaagat gtttaatatg agtcatgtct ttggctcttc ctgccattag
     4861 tttcatgatc aagacttctt cggggcccag actttttaca agtagctttt ttccaatata
     4921 gatatcggac attcgattct tagagtcctt aggaagatag actgtaaacg cctgatagta
     4981 aggattgagc cagtccggcg ctatcttgag ttcttttgcg acttcttcca taaagggctt
     5041 gagctcttca aattcaatat tagaaggtac agcatcaata tccaccgtag cgcctgagta
     5101 gtcatgtttg aggatcatcg cgcctcctcc gcctactgtt aaagtgacct taggtaaggt
     5161 ggaagtggcc agtttttcat ccagggtttg gagggctttc aaggctaaag ctttgttcat
     5221 gttcatatct aaattattct atttagaatg aaaaatgtaa agtatatttt ataaaaatat
     5281 aaccataagc tatataatag atttgatgct ttttttaatt ctataaatga gatccagaga
     5341 gaacgttcaa ctctaaattt taactctaac tttcgacttt aagtttgcag atcataactg
     5401 ccgatttggt gcagggcttt tttggcctct gccactgaga attccttttc aaactccaca
     5461 atcaagctgc catcaattaa aatgcgggga tcgatattga gtttgagaat ttccagatag
     5521 atagctttgg caatgggctt ctttttcacg ctgcgaagat aagtcatcag tgaagaaagc
     5581 aaaataaagg gatagaaaat caaaaataaa ataagattag agggcttcac ttctgagaag
     5641 tgaatcttgc ggattttaaa gccagcaatt ctagccagca gccgcatctt ggtaattcca
     5701 atcagaaaaa catgtccata gtactggttg tcgttgtgat cacgattgaa ccaaatgcta
     5761 tcgtactcat tcaccgccat aatgcggttg taacgctcgc tctcggttaa aagataggag
     5821 agcttgtgct taagattcga atgattagga gtcgtgataa tgagcgtccc ttgatccttc
     5881 atcacgcggt taaactcttt caatgcagcc aattgatccg gaaaatgctc aatgccttct
     5941 tggcaaataa tcaagtccgc cagttcattc gcaagaggaa ttccctctgt gatgtc
//
LOCUS       DATBTE010000004         1646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1776394, whole genome shotgun sequence.
ACCESSION   DATBTE010000004 DATBTE010000000
VERSION     DATBTE010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776394"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VIG33_00095"
     CDS             <1..789
                     /locus_tag="VIG33_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269258.1"
                     /translation="AEELNSYLARSRQNYAVLTEYSAIETYAPLDPAMTFQSMKTLGS
                     FPEQVIILKGAMDCCGIIGEPALYPEIFIEAGQTKGFRQFFRDLELAKHGSIQQCEGV
                     LKHGIAAKTQLARIHSSTEKVLEGLIDLSKTYTTAEIQAIRNGIGIRDMAVLNRATAN
                     ALDFAGLTFSTHPSVNRTPSFEELSDTFIFRHAICAQSLLLKWIESGSQVNLASMKIR
                     NDVVDINFATFALYFDGLLTKDKKLNSIYENAKRLLQYFRNENS"
     gene            802..1548
                     /locus_tag="VIG33_00100"
     CDS             802..1548
                     /locus_tag="VIG33_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269259.1"
                     /translation="MNYFNEWINWCDTHSGFLSLVLFLITLLIGWLGGFFKYIGQKPK
                     FTPSLIQGPSFVTIVPSDTKFEGRSTHRVVISLYLSIANTGSAAGSIFSISAAYKFPP
                     TSLKEFFKSRWNFIKETVALSDFSRQIGDHVKVYPFIRQKSFFFDSKGSTYFRVGEIH
                     NGITYFENENLAYGSHRPIVKNGLIKIKLKIIDSFGRQHIKVIPVPEVSVEAARLYSK
                     HIGNTHSSSASIPGMSPETAASKSIPSGAL"
BASE COUNT          479 a          346 c          331 g          490 t
ORIGIN      
        1 gccgaggagt taaattcata tttggcgcga tctcggcaaa actatgcagt acttacggag
       61 tattccgcaa ttgaaacata cgcgcctctt gacccagcaa tgacatttca atcaatgaaa
      121 actctaggct catttcccga gcaagtcata attttaaagg gcgcaatgga ttgctgtgga
      181 ataattggtg agcccgcgct ctatcctgag atatttattg aagcaggtca aacgaagggt
      241 ttcagacagt tctttcgaga tcttgaactt gctaaacatg gaagcattca acagtgcgaa
      301 ggcgttctga agcacgggat tgccgccaag actcaacttg cacgaattca ttcgagtaca
      361 gaaaaggttc tcgagggttt gattgatcta tcaaagacat acacgaccgc agaaattcag
      421 gcgattcgca atggaatagg aattcgggac atggctgttc ttaatcgagc aacagccaat
      481 gcccttgatt ttgctgggct tacattctcg actcatccgt ctgtcaatcg tactccctct
      541 tttgaagaac tttccgatac tttcattttt cgtcacgcta tttgcgctca atcacttcta
      601 ttaaagtgga ttgagagtgg gagccaggtc aaccttgcct cgatgaaaat acgcaatgat
      661 gttgtggaca ttaacttcgc aaccttcgca ctttatttcg acggacttct aacaaaagat
      721 aaaaagctaa attctattta cgaaaatgcg aagcggctct tgcagtattt tcgcaatgag
      781 aattcatgat ggaaaggcta tgtgaattat tttaacgaat ggataaactg gtgcgatacg
      841 cattcgggtt ttttaagcct tgttcttttt ttgatcacgc tgctaattgg ttggcttggc
      901 ggctttttta aatacatagg ccaaaagccg aagttcacac cttcccttat ccaaggacca
      961 tcatttgtaa caattgtacc tagtgatacg aagtttgagg gtcgctctac ccaccgcgtg
     1021 gtcatttctc tatatctatc aattgcaaat actggaagtg ctgctggaag cattttcagt
     1081 atatctgctg cttacaagtt tccacctaca tccctaaagg aatttttcaa atcacgatgg
     1141 aactttatta aggaaactgt ggccctttca gacttctctc gtcaaattgg agaccatgtt
     1201 aaagtttatc cattcataag acagaagtct tttttcttcg attcaaaagg ttccacttat
     1261 tttagagttg gcgaaattca taatggcata acttatttcg aaaatgaaaa tctcgcctat
     1321 ggctcccatc gcccgattgt gaagaatggg ttgataaaaa ttaaattaaa gatcattgat
     1381 tcatttggac gccaacacat taaagttata ccagtgccag aggtatctgt cgaagctgcg
     1441 cgcttatata gtaaacatat tggcaacacc cactcaagtt cagccagcat tccgggcatg
     1501 agtcccgaga cggcagcatc aaagtctatt ccctcaggag cattatgacc ggcataaata
     1561 aaaattttaa atactcaact tacattatcg cttttctgga tgttttggga ttccgagaga
     1621 tggttcaacg aattaaaaaa taatct
//
LOCUS       DATBTE010000005         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_410387, whole genome shotgun sequence.
ACCESSION   DATBTE010000005 DATBTE010000000
VERSION     DATBTE010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410387"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..1216)
                     /locus_tag="VIG33_00105"
     CDS             complement(257..1216)
                     /locus_tag="VIG33_00105"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY8269260.1"
                     /translation="MKTYLSYSARIVFYSLVCISTHASEVGATNQNPSPPLFTPANLN
                     KLELVSGMTKEQEIESENKVRAMEADASEQINLFENSLDSSLEAIKTSDNPSDVEISI
                     YQKTPGIKWTFLVAKENGKIRYVFATSPGMQGHSTPTSPSGVAWSVLSQAWRHTSTKY
                     PTPKGNMDHVSYFLPLYGLHSTVLGAYHKLGKPDSHGCVRLGRPEARALYRLIAMNQP
                     RVKIYSLKNQKPSEEDLKEILPLLAMDLNFIQKELLARNNSGDVPFKTAKQYLEYKAL
                     QEINDLEQVQKINKLFNIKSIQEIYSEQDRLPDLKILIPNHAT"
BASE COUNT          429 a          303 c          311 g          492 t
ORIGIN      
        1 ttcgataccg acaatcaagc cacataccac tgcaaaaaac attttaaagg aaatcaacgc
       61 tagaacttcc atctccgtca tttttagctc cttggattcc gctagattta ttagttatcg
      121 gactttatcc tgagaattga agcgagaacg cggtaatgga tttataaatt taagacaagc
      181 tcgaggggcc tcgttttaga tgtcactact gatgcccctc aacttaagta tgtggacttt
      241 cggtcagttt tatcccttaa gtcgcatgat ttggaatcaa aattttcaga tcaggtaagc
      301 gatcctgctc agagtatatc tcttgaatgg atttgatatt aaagagcttg ttgatttttt
      361 gaacttgctc cagatcattt atttcttgaa gagccttgta ttccaaatat tgtttcgctg
      421 ttttaaaggg aacatctccg gagttgttac gtgctaaaag ttctttttgg ataaaattta
      481 aatccatcgc caaaagaggg aggatttctt tgagatcttc ctctgaaggc ttttgatttt
      541 taagtgaata gattttgact cgtggttgat tcattgcaat caatctataa agggctcttg
      601 cttctgggcg tcccaaacgc acgcaaccat gtgaatctgg ctttcctaat ttatgataag
      661 cacctaaaac cgttgaatga agtccatata aaggcaagaa atagctaaca tggtccatat
      721 tcccttttgg agtgggatat ttagtagagg tgtgtctcca agcttggctc aagacgctcc
      781 aagcgactcc gctgggactc gtcggagtcg aatgaccctg catcccagga gaggttgcaa
      841 acacatatct gatttttcca ttctctttag cgacgaggaa cgtccactta attccagggg
      901 ttttttgata aatagaaatt tccacatcac ttggattatc tgaagttttg atcgcctcca
      961 gggaagaatc caatgagttc tcaaacaaat taatttgttc tgaagcatct gcttccattg
     1021 cgcgtacttt attttcactt tcaatctctt gttctttggt cataccagaa acaagttcta
     1081 gtttatttaa attagccgga gtaaacagag ggggtgaagg attctgattt gtagcaccga
     1141 cttcagacgc atgcgttgaa atacagacca atgaataaaa aacaatgcga gcagaataag
     1201 aaagatatgt tttcataatg atgctgccgt tccaaatcct gtaggagaat tttccagctt
     1261 gcaggggcac ggttcctttg acttagctcc catgcgagaa ccgttccaag cttcaattgc
     1321 cgataaaacc gttattacgc gaaaagactg cataaagttc ttacacagtg ccatgtgtgt
     1381 gagtcgtcgt cgttgcctct ccgtttttta gaatggggta gtttgttcca actgatgata
     1441 gaaaatatgc cagacctagg tctattggtt tttattaaaa caagtcttta ttctatccga
     1501 atgttttcat tggacttctg gttgtactca aacag
//
LOCUS       DATBTE010000006         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1571620, whole genome shotgun sequence.
ACCESSION   DATBTE010000006 DATBTE010000000
VERSION     DATBTE010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1571620"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VIG33_00110"
     CDS             <1..557
                     /locus_tag="VIG33_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269261.1"
                     /translation="SSLIDTLELHPDDLILLALSRPYIELLQDELLNCSPQQRQRLRF
                     FGKKLNSALPTSLVGNWMPYDDRLDCAGQDFSGTQSDFAQRALRHFVTEVLNMHQNGD
                     ASTHYSAVLELLSPFNRREIPKRQRLSDEEISTAIHNNWERGKGQSSTLLRIIRRELG
                     IACEQSRFRTIYHTVKNTMESTRQ"
     gene            554..1681
                     /gene="dbpB"
                     /locus_tag="VIG33_00115"
     CDS             554..1681
                     /gene="dbpB"
                     /locus_tag="VIG33_00115"
                     /inference="COORDINATES: protein motif:HMM:NF041060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein DpdB"
                     /protein_id="HEY8269262.1"
                     /translation="MKSEVINEIVVRALRTHQGDGIEVFAFFIYGSDIMKIADISRIT
                     RDEGDELKGFQRSEIKNHVKSITEYLNSNNALFPNSIILALGKEVDFKQSRGPTPTNC
                     VEVAQSGNLTIPVLPEGRRVAWIVDGQQRSLALAKAKNSRIPVPVVAFISPDIETQRE
                     QFILVNKAKPLPTRLINELLPEVDALLPRDLASRKLPSELCNLLNKDPKSPFYQLIRR
                     ESNAHSEHGVVVDSAILEVIKRNLRPPMGALSQFKGTHHSDPDAMYQTLVMYWSTVRD
                     TFPKAWGKAPSESRLMHSAGIKVMGALMDQIMLRADSTSHPDAEVRTSLARIAPHCRW
                     TEGIWEGLRWRWNEIQSTTQHISRLSEHLMRLDRELSRPTR"
     gene            1678..>2191
                     /gene="dpdA"
                     /locus_tag="VIG33_00120"
     CDS             1678..>2191
                     /gene="dpdA"
                     /locus_tag="VIG33_00120"
                     /inference="COORDINATES: protein motif:HMM:NF041059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase DpdA"
                     /protein_id="HEY8269263.1"
                     /translation="MKFIFSDSMDMVDPEFDFIEDRYAPNRRPYWDDVYPHEIFMTPP
                     YDGMLVSKAIVGDHRVKGNCYSESQAMRFYRVGAREFLRLNDPKYDNMPIFGDCGAFS
                     YAQADTPPYSPEEIVEFYNSCGFTHGCSVDHIIFDFSENCKDLEGGSEDAKRRFDITL
                     ENADIFLKAHQ"
BASE COUNT          631 a          527 c          459 g          574 t
ORIGIN      
        1 gttcctccct tattgacacc cttgaacttc atcctgacga cctcatcctc cttgctctat
       61 ctcgaccata catagaactt ctgcaagatg aactgctgaa ctgctctccc cagcaacggc
      121 aaagactacg cttcttcgga aaaaagttaa attccgcgct ccccacgtcc cttgtcggta
      181 attggatgcc atatgatgat cgcttagact gtgccggaca agatttcagt ggaactcaat
      241 ctgattttgc tcagcgagct ctgcgtcact ttgtaactga ggtgttgaat atgcaccaga
      301 atggtgatgc atctacgcat tactcagcag tactggaatt actctctcca ttcaacagac
      361 gagaaattcc caaacgccag cgactcagtg acgaagagat tagtactgcc atccataaca
      421 actgggaaag aggaaaaggt cagtcgtcta ctctattgcg tattattcgg cgagaattag
      481 gaattgcgtg tgagcaatcg cgtttccgaa ctatctatca cacagtcaaa aatacaatgg
      541 aaagcactcg acaatgaaat cagaagttat aaatgaaatt gtagttcgtg ccctaagaac
      601 ccatcaagga gatggaattg aagtctttgc gttctttatt tatggatcag acatcatgaa
      661 gattgccgac attagccgca ttacacgaga tgaaggcgac gaactaaagg gattccaaag
      721 gagtgagatc aaaaatcacg tcaagtcgat tactgagtac ttaaacagta acaacgccct
      781 ttttccaaat tcaatcattc tggcactagg aaaagaagta gacttcaagc aatcaagagg
      841 tcctacccca actaattgcg tagaggttgc tcaaagcggc aatctaacta tacctgtgct
      901 cccagaaggt cgccgagttg catggattgt tgatggccaa cagcgctcac tcgcactcgc
      961 caaagcgaaa aatagtcgca ttccagttcc tgttgttgcc tttatctctc cagatattga
     1021 aactcagcgc gaacaattca ttttagtcaa caaagcgaaa cctctaccaa cgcgactgat
     1081 caacgaacta ttgcctgaag tagatgcttt acttcctcgt gatctcgcaa gtcggaaact
     1141 gccaagtgaa ttgtgtaatc tccttaacaa agaccccaag tctccttttt atcaactcat
     1201 tcgccgcgaa tcaaacgctc atagtgaaca tggtgttgta gtagactctg ccatcttaga
     1261 agtcattaag agaaatcttc gccccccaat gggcgcacta agccaattca agggtactca
     1321 tcactccgat cccgacgcaa tgtatcaaac gctagtcatg tattggtcaa ctgtacgcga
     1381 tacttttcct aaagcttggg gaaaagcccc cagcgaaagt cggcttatgc actcagctgg
     1441 cataaaagta atgggcgcat taatggatca aattatgctc cgcgcagatt ctacatctca
     1501 tcctgatgca gaagtccgca cttcacttgc gcgaatcgct cctcactgtc gatggactga
     1561 aggcatttgg gaaggactta gatggcgttg gaacgaaatt caaagcacta ctcaacatat
     1621 ttcccgcttg agtgagcatt taatgcgtct tgatagagaa ctatcgcggc ctaccagatg
     1681 aagtttatat ttagtgacag tatggatatg gtcgatccag agttcgactt tattgaggat
     1741 cgctatgccc ccaatcgtcg cccttattgg gacgatgtct atcctcacga aattttcatg
     1801 actccaccct atgatgggat gcttgtatct aaagcaatcg taggggatca tcgagtcaag
     1861 ggaaactgct atagtgaatc acaagcgatg cggttttacc gcgttggagc tcgagagttc
     1921 ttgcgcctca atgatcctaa atatgacaat atgccgatct tcggcgactg tggcgccttc
     1981 tcgtacgcac aagcagatac ccccccatat tctccagaag agatcgtcga attttacaac
     2041 tcctgcggct ttacgcatgg gtgctctgtc gatcacataa tttttgattt ttctgagaat
     2101 tgtaaagatc tcgagggcgg ttctgaagat gcgaaacgaa gattcgacat cacattagaa
     2161 aacgcagata tatttttaaa agcccatcaa g
//
LOCUS       DATBTE010000007         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2391357, whole genome shotgun sequence.
ACCESSION   DATBTE010000007 DATBTE010000000
VERSION     DATBTE010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2391357"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..856
                     /locus_tag="VIG33_00125"
     CDS             335..856
                     /locus_tag="VIG33_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269264.1"
                     /translation="MIDAVELKTAADYLISIFNERKEKNPRYSLRAFARSLGVSSGQL
                     SEILSEKRPLSHKLARRISVALALTMNESQKLIYLVSQQSQFLESTGGNPRQLSEEEV
                     ALMTIAHNLDSNIKLNLDLQKAPETQVFSEIAQDHVLEVKKAIRNFRKKLKKIFEGSE
                     KKNIDLMSSENTI"
     gene            1392..>2063
                     /locus_tag="VIG33_00130"
     CDS             1392..>2063
                     /locus_tag="VIG33_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269265.1"
                     /translation="MPFAPGENSTSFDIHGLGFGKLKSVFFSSAPALPEAEGESANLV
                     RTWQPALPFQACIKPNRLFCFAGEATPITVNSGKKSQAEIDICGDANVKAYFEISRLK
                     SIQNLVSGVALPQKIAEQAARDKEYIENLVSKYEKEKGVKVNLNWLDKKTSLRCEIFK
                     NTEENLPDTQAGIFESEEAITIAVRGTEPGNERDIRTNVMTFNQVDLSASSSGTGSVH
                     RGFYKA"
BASE COUNT          669 a          402 c          393 g          599 t
ORIGIN      
        1 aaaaaataag ttccatggca tcttcttttt cctttctaaa atgtgagttg tttagcggtt
       61 cgaacacata tttttcttat tgaacaccaa aatctgtcgg tcaggccgat attttaatga
      121 caaaattaca tcaaaatgat cttaagttaa atattatata aaatactatc ttattgattt
      181 tacaaaactt ttaacttgta aaaaaataaa tatataaaaa tctgttgata actgttaagt
      241 ttttttgaca aattataatt taattgctcc aatatgaata acggactgac tgaaacattg
      301 taggaccaca atatttcaat acataagggt gtgtatgatt gatgcagtag agttaaaaac
      361 agcggcggat tatttgattt caatctttaa tgaaagaaag gagaaaaatc ctagatattc
      421 cttgcgagcg tttgccagat cgttaggggt ttcctcaggg cagctttctg aaattctctc
      481 agaaaaaaga cccctttccc ataaattagc ccgaaggatt tcagttgctt tggcgctgac
      541 catgaatgaa tctcaaaagc ttatttatct cgtgtcacaa caaagtcaat ttcttgaatc
      601 gactggcggc aatccacgtc aactctctga agaggaagtc gccttgatga cgatcgcgca
      661 taaccttgac tctaatataa agctcaatct tgatttgcag aaagcccctg agacccaggt
      721 tttctcagaa atagcccaag atcatgttct tgaggtaaaa aaagcaattc gcaatttccg
      781 taaaaaattg aaaaagatct tcgaaggaag tgagaaaaaa aatatcgacc ttatgagctc
      841 cgaaaataca atctagtttc aatgaatgcc tttatacaga ccgatagcac atatggatga
      901 aaaccgcgtg tcaaaaagtt cgatggacct tcgttcgatg caattcgact caggaggacc
      961 attcgcggca ctttgtaaag tgctcataca gccctattac ttctataact attttattcg
     1021 tagctaaacc ttggtatcaa agtcgcataa gggatcgacg ggagccgtga ctctgcgttc
     1081 ggtgtaggga attcaatccc atcatatgga gtgtgtcgca acttaaatag tttaaggaag
     1141 gcttcctaat gaaacttatt gcgattttac taataaatct tttatttact tcagctacta
     1201 cttacgctga agattacctg actcgccctg aggttaactg gaccagcgac tcaaaagttg
     1261 atttctgtgc gaactccagt acttttgatt ttcgtaatgc ctattggctg ggagctctct
     1321 cttattattc ctattggcat gtcaaatatg cgaatcccat aatttcctca aacccagagg
     1381 ccattctgga tttgcctttt gctccaggtg aaaattccac ttcctttgat attcacggcc
     1441 tcggattcgg caaattgaaa tccgtcttct tttctagtgc acccgcttta cctgaagctg
     1501 agggtgaaag tgcgaattta gtgcgaactt ggcaaccagc cttgcctttt caagcttgta
     1561 ttaaacccaa cagacttttt tgttttgccg gagaagccac acccatcacg gtaaattctg
     1621 gcaaaaagtc acaagctgag attgatatct gtggggacgc taacgtaaaa gcctactttg
     1681 aaatttctcg acttaagagt attcaaaatc tagtttcagg cgtggcatta ccgcaaaaaa
     1741 tcgctgaaca agccgcaaga gataaagaat acattgaaaa cctagtcagt aaatatgaaa
     1801 aggagaaagg tgtaaaagtt aacttaaatt ggctagataa aaaaacctca ctcagatgcg
     1861 agatatttaa aaatacagaa gaaaatctac ccgacacaca ggctggtatt tttgaaagtg
     1921 aggaggcaat aacgattgca gtgaggggta cagaaccagg aaacgaaagg gacataagaa
     1981 ccaatgtcat gaccttcaac caagttgatt taagcgcatc atcatctgga accggaagtg
     2041 tccacagagg tttttataaa gcc
//
LOCUS       DATBTE010000008         9578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_639, whole genome shotgun sequence.
ACCESSION   DATBTE010000008 DATBTE010000000
VERSION     DATBTE010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 9578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9578
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VIG33_00135"
     CDS             <1..403
                     /locus_tag="VIG33_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEY8269266.1"
                     /translation="FFLHTHEWTFQTFWLGLQIGFHATVLIAINNLRDAEQDRTVNKK
                     TLAVRMGKSFACYEIAFLSLAPFVMNYYWWTVSFKVAFLVPLLTLPLAVFIIKNIFQT
                     EPSSVYNRYLGQAALLHLTFGLLLSLGFYLE"
     gene            400..1392
                     /locus_tag="VIG33_00140"
     CDS             400..1392
                     /locus_tag="VIG33_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY8269267.1"
                     /translation="MKISYSPYTLKPVQYLNTVAHMGDRNGTLLKIEWPDEVVGYADL
                     HPWPELGDPSWEDQLADLRQGKISQMVEQSIWMARKDGQFRKSGKSAFETVPTIKNNY
                     IVVDVAAEPEWLADRLKADGFETVKIKVGRDLKKEAEFISLLGQDGAFQLRLDFNAIG
                     SWQTYERFMSSVNKVALQRVQYVEDPFPYDAQAWTEAKKFAPIAIDNQISKVDFKKLQ
                     GNPFDVVILKPAKMDVGRIIQYSIVHNLKVTVTSYMDHPIGVAHALAIASELKKSHPQ
                     RILDAGCMTLRLFQMEAYSAEMITTGPYIKRAAGKGIGFDLLLMKEPWAHLKIR"
     gene            1368..2615
                     /locus_tag="VIG33_00145"
     CDS             1368..2615
                     /locus_tag="VIG33_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8269268.1"
                     /translation="MGTPENSLNPQQIDWRSSENYILLNPRWSAKESQWLQEVAQSVS
                     LKRNLQGHLWLATSGSTSESFGFVKLVALAKEAFLTSARSVNRHLQASTSDTWLQVLP
                     RFHVGGLGVEVRSQLSQSQVIYDFAKWNPERIHKVIKDSKITLASMVPTQVFDLIQAK
                     LKSPPCLRVVIVGGGFLSETLYTEARKWGWPLLPSYGMTETCSQIATASLETLQKEKE
                     PIEMQIKMPLLQKLSHVEWRLNAEGLLQVQGSSLLTCYGQRRVDGSIHDWDPKEAGWF
                     TTEDLVSLQGDCMHFLGRKNEFIKIGGEGSSMGRLLEIFDRVLNSLDFSLHPSLKMSV
                     IQQVLLIDAPSKRLGTEIHLVTTLDFQSPPYQTLLSQLKENFNREVLPFERIREMKYT
                     SHIPRSDLGKVLSMQLKRELYGR"
     gene            2605..2940
                     /locus_tag="VIG33_00150"
     CDS             2605..2940
                     /locus_tag="VIG33_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEY8269269.1"
                     /translation="MAVEKQKTSWSEAVVLVCEKCGKSLDGLKLKEDGNASDNLKKYL
                     KSQLKQHGYGKQVRVVNSSCLDICIKNKVAVSYNPVDTSKHQQQTFTVHPEEDREQIL
                     NYLLGMLKK"
     gene            complement(2955..4004)
                     /locus_tag="VIG33_00155"
     CDS             complement(2955..4004)
                     /locus_tag="VIG33_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY8269270.1"
                     /translation="MSDSQKWLTLVLMAITAFLLYLLAPILMPFVAGFLLAYLANPVV
                     ERLSRWKIPRVAAVFIVFSAITLVLIILVGILIPGIRDQLVYLNTKMPEFIQWINTQA
                     IPWLENNFQIDLRHFDISTLTEALSSSWQEAGSLIGRLISKVAESGMNIVAFLASLAL
                     IPVVTFYLMLDWNNLITHISELLPRQIEGRVKALAKECDEVLGAFFRGQLLVMACLSF
                     VYCVGLKIVGLNIAIIVGLIAGVGSFIPYFGFTIGIVLATIGAIFQFQAWLPLLWVWT
                     VFGIGQVIENWVLIPYLVGNKVGLHPVSVIFAVLAGGQLFGFFGMLLAIPAAAVIVVL
                     MRGVRRQYFESQLYK"
     gene            complement(4001..4444)
                     /locus_tag="VIG33_00160"
     CDS             complement(4001..4444)
                     /locus_tag="VIG33_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY8269271.1"
                     /translation="METKTEWWRTGVQFQCQGSGQCCTSHGEFGYVFLTLEDRKRMAK
                     ILKMRTGDFTKKYCQKTGGIWHLIEEKGHPDCLFLEGNRCGIYEGRPTQCRTWPFWPE
                     IMEAKSWKKEVVNFCPGVNKGKVYTGEEIEKILSEQIKSEKELGT"
     gene            complement(4434..4859)
                     /locus_tag="VIG33_00165"
     CDS             complement(4434..4859)
                     /locus_tag="VIG33_00165"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY8269272.1"
                     /translation="MSLNFKTQIRIKFREADPAQIMYFANLFSLAHDVFEEFILATGY
                     QYQEWFSPLHHIIPLRHVEADYLAPLLPGQSYEVTAMVTNLGETSFKMAYKFAQNGKL
                     HGTVSMVHVILDPKTKAKMILPDLMKRRLSPYLETAHGN"
     gene            4976..5935
                     /locus_tag="VIG33_00170"
     CDS             4976..5935
                     /locus_tag="VIG33_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEY8269273.1"
                     /translation="MQRNVIDLKIYDSDDFPAYLPKLNSLYDDLFSGGKGFRAQMIRM
                     MGDSLKLEAKTELLLAQTIEFIHNASLLHDDLVDRSHLRRGKTSAWLKYTPEYAVLAG
                     DYLLARVMVNLSSHGNIKLVAYTSQVISDLLEGEWLQDSVVGDFFVTLEQLDRIHNLK
                     TASLFKWCLRAPFIALERYDEELHRTLEEMGTLLGQLFQRGDDLLDYNIRNHEGKAIL
                     GDLKSGYLNSFGAFVCEGLGRQQIDQLVRSKDLEGFYNSIGGKAPFDEKLKAFDTANE
                     KLISLYFHHMDRLKTHLKPGEEKLLTYLDPLTDLLYWRKKPRE"
     gene            5928..7013
                     /locus_tag="VIG33_00175"
     CDS             5928..7013
                     /locus_tag="VIG33_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEY8269274.1"
                     /translation="MSNVITLSKSSPEFESYLLGTFSKDKRALPIRTLNANSLQETVT
                     FEIKPVSEINKPNFAVLLIYLLKVRALILVLFPMYLILVKNTADSTIRDPFVVLLSSL
                     GILSAYIAVNLRNDYMDHMKGLDRINPKAGSRVIQRGWMTASQVKNWSNFFVGLALLL
                     AVLVIFAFPAVIGIVILTFSVGVWAQFLKRGSFKYQKGGEIFVFLLLGPLLTTGYHIS
                     IAGSWDLESLFLGMLWGWLVVFLQHIKNFEFILINSQARFHNTVTWLGFDKAKTFLKV
                     WWLCFLGMFIAYHLRYAGNFWTWFLGATLAFTAFPFFMSINYLQSPLGSDLVRVRRRG
                     YNLVLMTIFLWTLENVWYWARWRAWGL"
     gene            7001..7747
                     /locus_tag="VIG33_00180"
     CDS             7001..7747
                     /locus_tag="VIG33_00180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8269275.1"
                     /translation="MGTLGCIVFKEGALEEKALEKKVQRLADFLQIPVNPPDLQAFPF
                     FLEITLEGLRLIDSQRRVLEIDFQKDHLNYQRKGVRGKNELLAKALGYAKGTRKVLDL
                     SAGLGIDAVFMTQLGFQVTSVERSKLLFVLLDEALRKAPELQDKLHFVSGDSSEYLRS
                     PASVRDVDAIFFDPMYPHKKKTALPKQEMVVFRELVGNDEDAVKVLEEALRWPVNRVV
                     VKRPLGAEPLLPGVRHVYEGKVVRYDVYMR"
     gene            7750..8946
                     /locus_tag="VIG33_00185"
     CDS             7750..8946
                     /locus_tag="VIG33_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269276.1"
                     /translation="MMIMMWLSAIAMMIGSLSVFRLGIGQCFGTLQTRFLNLSESVPL
                     FKMLLTTKLCAVGQGSFVQSQYGAVALLNSRSFSKRYSILLLCLSSAGLWTTMMGILL
                     AWQINGEILLSVAVCVYLFYRWTKKGLGFLIILTGLGGFLVGAQWVLQKQSILLSVLG
                     ESEFHFLLADGRFPAQLLWLFVTFVLTLVIEIESWATLTALVLLVAGSLSLNGAVAII
                     IGEILAHVWVLSWRSRKLNQEAKMTAKSYAIASTVGLVIAFFAAGLLRDAFAWNFTFE
                     GNPLTEKSWQFLSFYLLIIIAQTLTVLLWGHFAAQKKLDEVQKGEYFSVRWISRGLVS
                     NKISDFILKKLKERLDLLLNQKKDLDSKERAQIPGAFLKVHEQEIAQLALWLPLAEAS
                     QKANRP"
     gene            complement(9190..>9578)
                     /locus_tag="VIG33_00190"
     CDS             complement(9190..>9578)
                     /locus_tag="VIG33_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269277.1"
                     /translation="DKIIRHHAKQCRIRLYHLVNVGNHLHLVIKLDDRKLYSKFIRAI
                     TGLIARQVLKAERGPAQGKSDLNIESQKKGFWVARPFTRLIAWGRDYNFITGYMNKNK
                     DQAQRFFTPWGFDILDPEFIQFLKTG"
BASE COUNT         2527 a         1944 c         2308 g         2799 t
ORIGIN      
        1 attttttttg catactcatg aatggacctt tcagacattt tggttaggct tacagattgg
       61 ttttcatgca acggtcttga ttgcgattaa caatcttcgc gatgccgagc aagacaggac
      121 tgtaaataag aaaactttgg cggtccgtat ggggaaaagt tttgcttgct atgaaattgc
      181 ctttctcagt ttggctcctt tcgtgatgaa ttattactgg tggacggtga gttttaaagt
      241 ggcgtttctc gttccgctcc taaccttgcc tttagccgtg tttattatta aaaatatttt
      301 tcaaacagag cctagctcag tttacaatag gtacttgggg caggcggcgc ttttacactt
      361 aactttcgga ttgctcctta gcttaggatt ttatttagaa tgaagataag ttacagtccc
      421 tacaccttaa agcctgttca atatcttaat accgttgccc acatgggaga ccgtaacggc
      481 actcttctta aaattgaatg gccggacgaa gtggtgggtt acgcggatct acatccgtgg
      541 ccggagctgg gagatccaag ctgggaagat caattggcgg atctccgcca agggaaaatc
      601 tctcagatgg tggagcaaag tatctggatg gctcgtaaag atgggcaatt tcgcaagagt
      661 gggaagagtg cttttgagac tgttccgaca atcaagaata attatattgt tgtcgatgtc
      721 gcggcggagc cagagtggtt ggcggatcgt cttaaggcgg atggttttga aactgtaaaa
      781 ataaaagtag gtcgagattt aaaaaaagaa gcggagttta tcagcctctt gggtcaagat
      841 ggtgcttttc aattgcgttt ggattttaat gccattggaa gttggcagac ctacgagcgc
      901 ttcatgtcga gcgtgaacaa agttgctttg caaagggtgc aatatgtaga ggatcccttt
      961 ccttatgatg ctcaggcttg gaccgaggcg aagaaatttg ccccgattgc aattgataat
     1021 caaatctcaa aagttgattt taagaagctc caaggaaatc cttttgacgt ggtgattttg
     1081 aaacctgcaa agatggacgt gggaagaatt attcaatatt ccattgttca taatctcaag
     1141 gtcacggtca ccagttatat ggaccatccc ataggcgtcg cccatgccct ggccatagca
     1201 agtgaactca aaaagtctca tcctcagcgc attttggatg cggggtgcat gaccttaaga
     1261 cttttccaga tggaggctta ctctgcggaa atgataacca ctggacccta cataaagcga
     1321 gctgcgggca aggggatagg ctttgatttg ctgctgatga aagagccatg ggcacacctg
     1381 aaaattcgtt aaatccgcaa cagatcgatt ggcggagttc cgaaaattat attttattaa
     1441 atcctcgctg gtccgccaaa gagagccaat ggcttcaaga agtagctcag agtgtttcct
     1501 taaagcggaa tcttcaaggt catctgtggt tagcgacttc aggttcgact tctgagtcct
     1561 tcggctttgt taagcttgtg gctcttgcca aggaggcgtt tttaacttct gcaagatcag
     1621 tgaacagaca tctgcaggct tcaacctctg atacttggtt acaggtttta ccaagatttc
     1681 atgtgggagg cttgggcgtt gaagtgagga gccagctttc acaaagccaa gtgatttatg
     1741 attttgcaaa atggaacccc gagcgcattc ataaagttat taaggattct aaaattacgc
     1801 ttgcttccat ggtgccaact caagtgtttg atctgattca agcgaagctc aagtcgccgc
     1861 cctgtttaag agttgtgatc gtaggggggg ggtttttgag tgagaccctc tatacggaag
     1921 caaggaagtg gggctggccc ctattgccaa gttacggaat gacggaaacg tgttcacaaa
     1981 tcgccacggc ttctttggag actttacaga aagaaaaaga gcccattgaa atgcagatta
     2041 agatgccgct gctgcaaaag ttaagtcatg tggagtggcg tttgaatgca gagggccttt
     2101 tgcaagttca aggttcttca ctgttaactt gttatgggca gcgccgagtg gatggcagta
     2161 tccatgattg ggaccccaaa gaggcggggt ggttcacaac cgaagatttg gtgagtcttc
     2221 aaggggattg tatgcatttc cttggacgca aaaatgagtt catcaaaatt ggcggtgaag
     2281 gcagctccat gggtcggctt ttagaaatct ttgacagagt tttaaactct ctggattttt
     2341 cccttcatcc ttctctcaag atgtctgtta ttcagcaggt tttattaata gatgctcctt
     2401 cgaaacgatt aggaacagaa atccatttag tcacaactct ggattttcag tcacctcctt
     2461 atcagacgct cctttctcaa ttgaaagaaa actttaatcg tgaggtgctt ccttttgaaa
     2521 gaatccgtga aatgaagtat acttcgcata tccctcgttc tgacttggga aaggttcttt
     2581 caatgcaatt gaaaagagag ttatatggcc gttgaaaaac aaaaaacctc ctggtctgag
     2641 gccgtagttc tcgtttgtga aaaatgtggc aaaagcctcg atgggttaaa actcaaagaa
     2701 gatggcaatg cttctgataa tcttaaaaag tatttaaaga gtcagctaaa acaacacggt
     2761 tatggcaaac aggtgcgggt cgttaattca agttgtcttg atatctgcat aaaaaataag
     2821 gtcgcagtca gttataatcc tgtggatacc agcaagcatc agcagcaaac ctttacggtt
     2881 caccccgaag aggatcgtga acaaatttta aattatcttc tggggatgct gaagaaatag
     2941 gtagcccgaa gcctttattt ataaagttga ctctcgaagt attgccttcg tacgcctctc
     3001 atcaagacca cgatcacggc tgcagcgggg atcgccagga gcattccaaa aaagccgaag
     3061 agctgacctc ctgccaaaac cgcgaaaatc acggagaccg ggtgaagccc gactttattt
     3121 cctactaagt aaggaatcag cacccaattt tcaatgactt ggccaatacc aaataccgtc
     3181 cacacccaca ggagggggag ccacgcttgg aattggaaaa tggctccgat ggtagcgagg
     3241 acaattccaa tggtgaaccc aaaataaggg atgaaactgc caacaccagc gatgaggcct
     3301 acgataatcg ctatattaag gcctacgatt ttaagaccta cacaataaac aaagcttaag
     3361 caggccatga ccaaaagttg ccccctaaag aaggcgccca acacttcatc acattctttg
     3421 gcaagggctt tgactcgccc ttcgatctgg cgaggcaaaa gttcgctgat gtgagtgatg
     3481 agattattcc aatccagcat gagatagaaa gtgaccaccg gaataagggc caagctggct
     3541 aggaaggcca caatattcat tcccgactcg gccaccttgg aaataagccg gccgataagc
     3601 gagcccgcct cttgccaaga gcttgaaagt gcttcagtca gagtggaaat gtcaaagtgc
     3661 ctcaggtcaa tttgaaaatt gttttcaagc caaggaattg cctgggtatt aatccactgt
     3721 ataaattccg gcattttagt atttaaataa acaagttggt ctctgatgcc tggaatgagt
     3781 atgcccacca aaataatgag cactaaagtg attgcggaaa atacaatgaa cacggcggca
     3841 acgcgtggaa ttttccagcg actcaggcgt tccaccacgg gattggccag ataagctaaa
     3901 agaaagcccg ccacaaaggg catcaagatg ggagctaata aatacagcag aaacgcggtg
     3961 atggccatca gcaccaaggt caaccatttt tgagagtcac tcatgtgccg agttcctttt
     4021 cgcttttgat ttgttcactg aggatttttt caatttcctc accagtatag actttaccct
     4081 tattaactcc ggggcagaag ttgacgactt cttttttcca ggattttgct tccatgatct
     4141 cgggccagaa aggccatgta cgacattgcg tcggacggcc ttcgtagatg ccgcagcggt
     4201 tgccctcgag gaatagacag tcggggtggc ccttttcttc tattaaatgc caaatccccc
     4261 cggttttctg gcagtatttc ttggtgaagt ctcccgtgcg cattttgaga attttagcca
     4321 tgcgcttgcg atcttctaaa gttaaaaaca cataaccgaa ttccccatga gaagtgcagc
     4381 actggcccga accttggcac tgaaattgaa cgccggttct ccaccattca gttttagttt
     4441 ccatgtgcgg tctccaaata agggctcaaa cgccgtttca ttaaatcagg taaaatcatt
     4501 tttgccttcg tcttagggtc aaggatcaca tggaccattg aaactgtccc gtggagtttt
     4561 ccattttgtg caaacttata ggccatttta aaggaagttt cccctaaatt tgttaccatc
     4621 gcagtgactt catagctctg acccggaagg aggggggcca ggtaatccgc ctcgacatgg
     4681 cggaggggaa tgatatgatg gagggggcta aaccattctt ggtactggta accggtggcc
     4741 agaatgaact cctcaaagac atcatgggca aggctaaaga ggtttgcgaa gtacatgatt
     4801 tgggctggat cagcctcacg gaatttaatc cgaatttggg tcttgaaatt caggctcatc
     4861 aactggctcc ttttttgact tgctcctttt taacttttaa gatgaaggac tccttgacag
     4921 caagcatata aatttgaaat cttacggcct tttcaaccgg tcttaggagc ttatcttgca
     4981 gcgcaatgtc atagatctca aaatctacga ttctgatgat tttccagcct atttacctaa
     5041 attgaactct ctttatgatg acttgttcag tggcggcaaa ggttttcgcg cccaaatgat
     5101 tcgcatgatg ggggattctt taaagctgga agctaaaact gagttacttc tggctcaaac
     5161 catcgaattt atccataacg cttctttgct tcatgatgac ttggtcgata ggtcacatct
     5221 tcgtcgtggt aaaacctcgg cgtggttgaa gtacactcct gaatatgcgg ttcttgccgg
     5281 agactatctt ttggcgcgag tgatggtgaa tctttcaagc catggcaata tcaaattggt
     5341 ggcctacact tctcaagtga tctcggactt gctggaaggg gaatggcttc aggactcagt
     5401 agtgggggat ttctttgtca ccttggaaca gctcgatcgt atccataatt tgaaaacagc
     5461 ctctttgttt aaatggtgtt tgcgtgctcc gtttatcgcc ttagaaagat atgatgaaga
     5521 gttgcataga acccttgaag agatgggcac cctcctcggt cagctttttc aacggggcga
     5581 cgatttattg gattataata ttcgaaatca tgaaggcaaa gctatcttgg gtgatttgaa
     5641 gtcagggtat ctgaactcat tcggagcttt tgtgtgcgaa ggcttgggtc gacaacaaat
     5701 cgatcaattg gttcgttcga aagatcttga aggtttctat aatagcattg gtggcaaggc
     5761 cccattcgat gagaaactca aagccttcga tacggcaaat gaaaaactga taagtcttta
     5821 tttccaccat atggatcgat tgaaaactca cttgaaacca ggggaagaaa aacttttgac
     5881 gtatcttgac ccgctaacgg atcttctcta ttggagaaag aagcctcgtg agtaacgtaa
     5941 taacactttc caaaagctct ccggagtttg aatcttatct tttaggaact ttttctaaag
     6001 acaaaagagc tttgcctatt cgcactttga atgcgaattc gttgcaagag accgttactt
     6061 ttgaaattaa gccagtcagt gaaattaaca aaccaaattt cgcggtttta ttaatttatt
     6121 tgttgaaagt acgagcctta attttggttt tgttcccgat gtatttgatt ttagttaaaa
     6181 acactgctga tagcaccatc cgtgatcctt ttgtggtgtt gctgagctcg ctaggaattc
     6241 tttcagccta tattgccgtg aatcttcgca atgattatat ggatcatatg aagggccttg
     6301 atcggattaa tccaaaagcg gggagccggg tgattcaacg aggctggatg acggcaagcc
     6361 aagtaaaaaa ttggtcgaat tttttcgttg gcttggcact attattggct gtactggtga
     6421 tctttgcttt cccggccgtc atcggtatcg taatactgac cttttctgtg ggggtctggg
     6481 cgcaattttt gaaaagagga tcttttaaat atcaaaaagg cggagaaatc tttgtcttcc
     6541 ttcttttggg tcctttactg acgacgggtt atcatatttc catcgcgggc tcctgggatt
     6601 tagaatcgtt gtttttaggg atgttatggg gatggctggt agtcttcctt cagcatatta
     6661 aaaattttga atttattttg attaactccc aggctcgttt ccataacacc gtgacttggt
     6721 taggctttga taaagcgaag acttttctta aagtctggtg gctgtgcttt ttgggaatgt
     6781 ttatcgcgta tcatcttcgc tatgccggaa acttttggac ctggttttta ggggcgaccc
     6841 tggctttcac cgcctttccg tttttcatga gcatcaatta tttgcaaagc cctttagggt
     6901 cagacctggt ccgcgttcgc cgccgtggtt ataatctagt gcttatgaca atctttttgt
     6961 ggacccttga gaacgtttgg tactgggccc ggtggcgggc atggggactc taggttgtat
     7021 tgtttttaaa gaaggagctt tggaagagaa agctttggaa aaaaaagtac agcgtcttgc
     7081 tgacttcttg caaatcccgg tgaaccctcc agaccttcaa gcttttccat tttttctaga
     7141 gatcactttg gaaggattgc gtctcatcga ttctcagcga cgagttctgg aaatcgattt
     7201 tcaaaaagat catttgaatt atcagcgtaa aggagttcgt ggtaaaaacg agttgctcgc
     7261 taaagctctg ggatacgcca aagggactcg gaaggttttg gatttgtccg cgggccttgg
     7321 cattgatgct gttttcatga ctcagttggg ttttcaagtg acatcggtgg agagatccaa
     7381 actgttgttt gttctcttag acgaagcttt gcgaaaggct ccggaacttc aagataaact
     7441 tcactttgtg agcggcgaca gttccgagta tttgcgttct cctgcgagtg tccgcgacgt
     7501 tgatgctatt tttttcgatc ccatgtatcc tcataaaaag aaaacagctt tgccgaaaca
     7561 ggaaatggtg gttttccgcg agctggttgg caatgatgag gatgcggtaa aagttctgga
     7621 agaagctctg cgatggcccg tcaaccgagt ggtggtcaaa cgtccattgg gagcggaacc
     7681 acttttaccc ggcgtacgtc atgtctatga aggcaaagtc gtccgttacg atgtttatat
     7741 gaggtgaata tgatgattat gatgtggcta agtgcaatag caatgatgat cggaagctta
     7801 agcgtttttc gcttgggcat cggccaatgt ttcggaacct tgcaaacgag atttttgaat
     7861 ctttcagaat cagttcctct ctttaaaatg ctgctcacga cgaagctttg tgctgttggc
     7921 cagggaagtt ttgtccaatc tcaatatggt gccgtggctt tattaaactc ccggagtttt
     7981 tcaaaacgat attccatttt attgctctgc ctcagcagtg cggggttgtg gactacgatg
     8041 atggggattc ttttggcgtg gcagatcaac ggtgaaattc ttttatctgt ggccgtgtgt
     8101 gtttatctct tctaccgctg gactaaaaaa ggtttgggct ttttaattat tcttaccggc
     8161 cttgggggat ttctggtggg ggctcagtgg gttttgcaaa agcaatccat tttattatcg
     8221 gtccttggcg agagtgagtt tcatttttta ttggctgatg gtcgatttcc tgcccagctt
     8281 ctttggcttt ttgtcacgtt tgttttaact ttggtgatcg agattgaatc ctgggcgacg
     8341 ttgacagcct tggttctttt ggtggctggg tctttgtcct tgaacggagc tgtggcaata
     8401 atcattggcg aaattctcgc ccacgtgtgg gtcctttcct ggagatctcg taaactcaat
     8461 caagaagcga aaatgacggc aaagtcttat gccatcgcca gcacggtggg attggtgata
     8521 gccttttttg ccgcaggcct tctgcgcgac gcctttgcct ggaacttcac ctttgaagga
     8581 aatccactca cggaaaagag ctggcaattt ctaagttttt atcttctgat cataattgct
     8641 caaactctga cggttctttt atggggccat ttcgcggctc agaaaaaact ggatgaagtt
     8701 caaaaaggag aatacttctc ggttcgttgg atttccagag gcttggtctc gaataagatt
     8761 tccgatttta ttctcaaaaa attgaaggag cgtttggatc tgctactcaa tcagaaaaaa
     8821 gatttagaca gcaaagaacg tgcgcagatt cctggggctt ttttaaaagt tcacgagcag
     8881 gaaatagctc aattggcatt gtggcttccc ttggcggagg ccagtcagaa ggcgaatagg
     8941 ccctgagttt tttcgaagct ttttaggaga gagtggagtt aattaggctt tgaggtcagg
     9001 gaaaagaaag ataggcttgg tttgcataag tcgcgaagac gcttgttgaa taaaaagaga
     9061 ctttgggcgc gactttactt ctgattgcgt acaatcagaa gttcaaaaaa tagaaataat
     9121 tttggaacta ataatagaaa caacaacaga tattacgaca gaaatgtttc tttggaataa
     9181 gcattaaatt cacccggttt taagaaattg aataaactca ggatccagga tgtcaaagcc
     9241 ccagggagta aaaaaccttt gggcttggtc tttatttttg ttcatatagc cggtaataaa
     9301 attataatct cttccccagg caatcaaacg ggtaaagggt ctggccaccc agaaaccctt
     9361 cttttggctt tcgatattta agtcgctctt tccctgagct gggccgcgtt cggcctttaa
     9421 gacttgccgg gcgattaagc ccgtgatggc acgaatgaac ttcgagtaga gcttgcggtc
     9481 atcgagtttg atcaccagat gaaggtgatt tcccacattc actaaatgat agagacgtat
     9541 gcggcactgt ttagcatggt gtcggatgat tttatcaa
//
LOCUS       DATBTE010000009        12545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_888768, whole genome shotgun sequence.
ACCESSION   DATBTE010000009 DATBTE010000000
VERSION     DATBTE010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 12545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12545
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888768"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="VIG33_00195"
     CDS             complement(<1..1101)
                     /locus_tag="VIG33_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269278.1"
                     /translation="MNSAANSNPNLKAPPNSKIRLEIAQPSDNQAILEFYKSFPVKGV
                     LDLKVNRCGDFFAPYATESDRFRTYLLKDTQNRVQGMASFIIKDVLIDGEPKTVAFGR
                     DLRISNNRRAILEWGDHFVPTLQDITASYGVQHFFSALNMTETSAFNAFVRPHNLKRL
                     LPRYYSYRHFNLISVHGRLPWAKKPLPQMQIRHGSSVNADALIYYLSRKNRERNLATY
                     WDSYSFFKQLQRWRNLKIEDFLIAFDRHNNIVGCCAPWKSDGIQEFIPLEYSLKAHNF
                     RQFLKFGKLLGWTRSLTKPSYRLQLEESMQFQYLTHLFADNADIFESLLWVAYDRARA
                     KDFLIYSHTREELIYKRPRSWACAKIPHCLYSM"
     gene            complement(1098..1664)
                     /locus_tag="VIG33_00200"
     CDS             complement(1098..1664)
                     /locus_tag="VIG33_00200"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY8269279.1"
                     /translation="MNAKIYTKTGDKGNTRLVDGSCVEKFNPRVEAYGTVDELNSYLG
                     VVRGGLQKLQELHSLDPIFEKIQNQLFNLGSLLACADETIFKRLPTLEERDVQFLEQK
                     IDELSVELSPLSHFILPAGHEVATHLHVARTICRRSERRSAEISMEEERYARSLQYLN
                     RLSDFLFVAARWVNLKTHFADVLWKKDE"
     gene            1737..2555
                     /locus_tag="VIG33_00205"
     CDS             1737..2555
                     /locus_tag="VIG33_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEY8269280.1"
                     /translation="MQLPPYSLLVTIPHSGEKVPPQAPWLKSLPEEILMCDVDRYVDF
                     LYEPSLQKLHLPYVKTEWHRYAGDLNRLPEDVDAGSVEGHINPAGMHRRGFLWTITTY
                     NNQLMSKPVSVKTYRELVELIYEPFHARVRKLYENFEREGRTKVFHIDAHSMPSVGTA
                     EHRDPGERRADIVVSDCHGQSCDPQFRDLVIAAYVVAGFKVAYNWPYFGGRLSEHYGR
                     PAQRHHAIQVEMNRGLYMDETTKKLKSAEAQKIQAKVELALSYICQQMLNLQRG"
     gene            2627..4057
                     /locus_tag="VIG33_00210"
     CDS             2627..4057
                     /locus_tag="VIG33_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEY8269281.1"
                     /translation="MSDNNNDVVDALKRRLRRLIKFLASLKLAVVVIAALACVIAAGT
                     LIESKYDATAARKMVYETFWMYSVMGVLATNLIAVMVDRWPWKRRHTPFIFAHIGILI
                     LMLGAVITQKYGLDGSVRVGIGESNQYVIVPETDILVYTSFDGDRYTKLAEQNVDFFT
                     TPPTPQKPLIVTGYNSDIKFTAYKPYVIPSHKVTVPKELSGRRGSALRFQIQSEKIKV
                     NVVEWIVQRRPNEVATHDFGPAQLHLGLAPAKGRGANEIYFTPDSAKDGLKYVVFKKD
                     SEEPVKTGFIKEGDIFNPGWKMPIEVRALRYLPEAVEEWDLQDRASPTPLTTSAVKLE
                     FKGQDHWLLLNDTVKLFTQEVVYIVSYGNRRLDLGFPIRLKNFEIGRYQGTAKAMAYK
                     SMVEVPDLAGTQEISMNEPLKYRGFTIYQASFQEDPKSQMPTASIFSVNQDPGRWLKY
                     LGCFIMVLGIIMLFYFRKKAKKESPV"
     gene            4054..4608
                     /locus_tag="VIG33_00215"
     CDS             4054..4608
                     /locus_tag="VIG33_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269282.1"
                     /translation="MRRLQFILVLLLSMLIYKDVFAAPGDEMRYLPVQDAGRIKPYDT
                     FSKEVLELIYGKSTFEDHGAPEIIFTWMLSPTAWQSKKIFEVRNHEVLKALKLPIEQR
                     YFTGDELFANERFSLLLQELQAKRESKEKLTPYFQALQRLENQLMVFREVAGGNMLRL
                     IPPKEGNRWLSVADFQGEQQAAFG"
     gene            4609..5685
                     /gene="ccsA"
                     /locus_tag="VIG33_00220"
     CDS             4609..5685
                     /gene="ccsA"
                     /locus_tag="VIG33_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY8269283.1"
                     /translation="MTKAFIEHIGALAKGSPAEQVTQAALALDASVLKFREQAKSNNP
                     ELYSQESKVKYEVFYNHFHPFRWAYILYMLASVVILIVWSMDKPGVMKFAWVLACSGF
                     ALHTFGFGIRMYLMDRAPVTNMYETVVWMSWGAVVFSSVLEIIYRYRFIFFAGTLGAM
                     VSLIIADSAPAVLDPSLQPLEAVLRSNYWLTIHVMTITISYAAFFLAFVLGDIGLIYY
                     IRGEQSHKEKIQAIAVSVYRAMQIGICFLAPGIILGGIWADYSWGRFWGWDPKETWAL
                     IALLGYLAVLHGRLGGWFKNFGTIAAGVVTFSLVIMAWYGVNFVLGAGLHSYGFGAGG
                     VEYVAAFIGAHFLLVLYASLVRRK"
     gene            5882..6400
                     /locus_tag="VIG33_00225"
     CDS             5882..6400
                     /locus_tag="VIG33_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY8269284.1"
                     /translation="MHRVFRTFAAVGLLALSLAGCKFQPGWGYNQGYAPEQPLPFSHE
                     IHVTKNQMQCQYCHNQVERSKHSSIPALSTCMNCHLQVKTDSPFIQKLREAYDKGGSI
                     EWVRVHMLPDHVQFNHSAHIAKGVNCQTCHGEVEKMAKITQVQDLSMGWCVNCHRQPE
                     NKAPLNCTTCHH"
     gene            6418..9558
                     /locus_tag="VIG33_00230"
     CDS             6418..9558
                     /locus_tag="VIG33_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEY8269285.1"
                     /translation="MSENNHEHMHEHSESELSAELAAKKAARPQIERNNTHWLTLEHY
                     NNDPEFMKKAETEFQSSPLRESESEGGWARREFLKLMGASLAMSAAGCIRRPVQKIVP
                     YNKQPEEVTLGVANYYASTYFDGNEGLGVLVKTREGRPIKIEGNPKFPMNKGGLSVRA
                     QSSILSLYDPERLQGPKHNIFNEKRSNKDTVSIKWDGLDDKVVAQLKKGGAVVLTGNV
                     ASPSTRAVISDFAQAFKADHVSWEPLANEEIREGQRASYGEAVVPQYRFDKAKMIVSI
                     DADFLGTWLNPTTFGAQFAAGRRDPKKLSKLVSFDSTYSLTGGNADIRVKIKPSQQLD
                     VVMGLINVLVVNKNLSAYANNEKFKDSLVSYANTAQKLGIEPALFTKVATDLWENKGQ
                     SLVVAGGLQTLTSRSAELQIAVNFLNSLLENEGKTVDGKNGNPGLKASYAELEKLIKE
                     MNAGKVKTLIIHRVNPMYALPKEAGFAEALKKVDMVVYTGDRIDETGEHATYLAPDNH
                     PLETWGDAEFAKDIFAIQQPAIRPLYDTRSFQLSLMTWAYLANVGPKRLITFETFYDY
                     LRNFWKEEIQPKAAKGKAFEDFWAEVLQNGYVGDLDHSTAARSFKSEAFAAIKPASNR
                     SGFELALYPTVQHGDGSSANNSWQQELPDPVTKIVWDNYVCVSIATAEKYHLKQSTVV
                     ELKVGDKKVELPVHIQPGLHDDVLAVAVGYGRTRAGRVADGVGQNMFEFASFAGGPVF
                     AGQEASFTKTTKKYNLASVAGNNSMEGRMIVAEATLKDYMKNEHAGVHRHKTWSIWSG
                     HQYNGHKWGMSVDLNTCTGCSACMTACQSENNISVVGKTYVLQGREMHWMRVDRYYVG
                     NPADALAVFQPVMCQHCDNAPCETVCPVLATVHSDEGLNEMVYNRCVGTRYCANNCPY
                     KVRRFNWFNYAKLIEKPMHMALNPDVTVRPRGVMEKCSFCVQRIKEAKNKAKLEDRKL
                     KDGDVKTACQIACPTQAIIFGDLNDPESQVAKIFKDEPRSYALLEEWHAAPSVRYLSK
                     IRNNDKETPAAEKGGH"
     gene            9558..10925
                     /gene="nrfD"
                     /locus_tag="VIG33_00235"
     CDS             9558..10925
                     /gene="nrfD"
                     /locus_tag="VIG33_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEY8269286.1"
                     /translation="MIHRNKLVLGDKDLKDITEDICAPIEAYPSKGWLGLLTGALSIL
                     IFYVIILGIVIGKGIGLLGVNHPNAWGTMIVTFVFWIGIGHAGTLISAILFLFRQKWR
                     TSVARTAEAMTVFAVMTAGLFPLLHTGRPWLDYWLFPYPNQRGPLWVNFRSPLLWDVF
                     AVSTYATVSMVFWYVGLIPDFATIKDRAKNKIRRTVYGALSLGWRGTAKNWSHYEMLY
                     LLLAGLSTPLVLSVHTIVSFDFAVSSLPGWHTTIFPPYFVAGAIFSGFAMVVTLMTLV
                     RIGVPEFKNYITIDHMEVMNKIIMSTGLMVGYAYGSEFFIAWYSGNPYEKYVFFVSRA
                     FGPYAWSYWTMITCNVLIPQVFWFKKARRSIPIMFVVSIFVNIGMWFERFVITITSLH
                     RDFLPANWGNYAWSFYDGAILFGSFGMFLTLFLLYLRFFPAISIAEIKPVLPVGRESK
                     GGTNG"
     gene            10918..11445
                     /locus_tag="VIG33_00240"
     CDS             10918..11445
                     /locus_tag="VIG33_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEY8269287.1"
                     /translation="MAKHGPGGIAGIWLDEHTVVKAAAKTRESGFVKFDAITPYPVHG
                     MEEACGIKRSWIPYVTFGAGAVGLLSGLWLTWWTSAVDWPINVGGKPFFSLPAFIPVI
                     FELTILFAALTSVGALFYVTKIPRLDPPNIDPDLTSHKFAIFIPENDIGYDESKIERF
                     FKENGAVEVKKMAEY"
     gene            11447..12010
                     /locus_tag="VIG33_00245"
     CDS             11447..12010
                     /locus_tag="VIG33_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8269288.1"
                     /translation="MRSQQIEKIILIAAATLAFSACGAKGDKSNVELIQDMMESPAIK
                     SQEYDESSPNHSGMRIPPDNTAPVGFAPYKFKGNLEGADKNVNPLAGNMTPDVLKVGQ
                     KYFETHCTVCHGQTGAGNPDSNVVKLMALKPPLILSDKVKGWTDGHIYHVISEGQGVM
                     GPYASHIPQAYRWQVVNYIRFLQKESK"
     gene            12020..>12545
                     /locus_tag="VIG33_00250"
     CDS             12020..>12545
                     /locus_tag="VIG33_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase"
                     /protein_id="HEY8269289.1"
                     /translation="MAVSNHSNELHVGKFEASSKLKITSYVLIAIGLITFAMGLMKDQ
                     ERIWAAYLVSFFFFSCVGLGGLFWTSLQNLVKAGWSVSIRRYAEGLSSFIPVMVVASF
                     VLILGLKYLYPWADHEKVAESALLQSKSTFLNTTFLIIRLVVFGLGMLAFRQVIVGNS
                     LKQDKSGDEQLTHKN"
BASE COUNT         3266 a         2571 c         3086 g         3622 t
ORIGIN      
        1 catggaatac aggcaatggg ggatttttgc acaagcccag ctgcgaggcc gtttatagat
       61 gagctcctcg cgagtgtggg agtagattaa gaaatcctta gcgcgggcgc gatcataggc
      121 cacccaaagg agggattcaa agatatctgc attatcagca aaaagatggg ttaagtactg
      181 aaattgcatg gattcttcca gttgtaggcg ataagagggt ttggttaaag agcgagtcca
      241 accgagaagt ttcccgaact tcaaaaattg gcgaaaatta tgtgccttca gagaatactc
      301 aagaggaata aactcttgaa ttccatcaga cttccaagga gcgcaacagc caacgatatt
      361 gttatggcga tcaaaagcga tcaaaaagtc ttcgatttta agattgcgcc aacgttgaag
      421 ctgtttaaaa aaactgtaag aatcccaata agtcgcgaga ttgcgttcgc ggtttttcct
      481 ggataaatag taaattaagg catccgcatt cactgaagag ccgtgacgaa tttgcatttg
      541 aggcaaaggt ttttttgccc agggaaggcg gccgtggacg ctgatcaggt taaaatggcg
      601 ataactatag tatctgggaa gcagccgttt aagattatga ggtctgacaa aggcattgaa
      661 agccgaagtt tctgtcatgt tgagtgcgct aaagaaatgt tggactccat aggaggcagt
      721 aatgtcctgt agggtaggga caaaatggtc gccccactcc aggatagcac ggcgattgtt
      781 gctaatgcga agatcacggc caaaggccac ggtttttggt tcgccgtcga tcaaaacatc
      841 tttgatgatg aaagaagcca ttccttgaac gcgattttga gtgtctttca gtaaataagt
      901 acgaaagcga tcggactccg ttgcataggg agcgaaaaag tctccgcagc gattcacctt
      961 aaggtctaag acgcccttga ctggaaagct cttgtagaac tctaaaatag cttggttatc
     1021 tgaaggttgg gcgatctcga gtcttatttt tgagttaggg ggggctttca agtttggatt
     1081 tgaattggct gccgagttca ttcgtctttc ttccaaagaa catctgcaaa atgagttttt
     1141 agattcaccc accgagcggc cacaaataag aaatcactga ggcggtttaa atactgcaag
     1201 ctccgcgcat agcgctcctc ctccattgaa atttcagcgg atcttctttc gcttctgcgg
     1261 cagatggtgc gggctacatg taaatgagtt gcgacctcat gcccggcagg taaaataaaa
     1321 tgactcagag gcgatagctc cacactcagc tcatcaattt tttgctctag gaattgcaca
     1381 tcgcgctctt caagcgtggg aagtcttttg aaaatagttt cgtcggcgca agctaaaaga
     1441 ctgcccaggt tgaaaagctg attttgtatt ttttcaaaaa tcggatccag ggaatgaagc
     1501 tcctggagtt tttgcaagcc tccgcgaacc acccctaaat aagaattgag ttcatccacg
     1561 gtgccatagg cttcgacgcg ggggttgaat ttttcgacac aggagccatc cactaagcgt
     1621 gtgtttcctt tgtcgccagt ttttgtataa atttttgcat tcatggtttt ctcaccctaa
     1681 acttagcctt aaacatataa ttagaatggg cacagcatga cattaaggag tttacaatgc
     1741 aacttccccc gtattcgctc ttggtgacaa ttccgcactc cggtgaaaaa gtccctcccc
     1801 aggccccttg gttgaaaagt cttccagaag aaattttgat gtgcgacgtg gatcgttatg
     1861 tggactttct ttatgagccc agccttcaga aattacatct tccgtatgta aaaacggagt
     1921 ggcatcgtta tgccggagac ctcaatcgac tgccagaaga tgtggacgca ggttctgtgg
     1981 aaggccatat caatcctgca gggatgcacc gccgtggatt tttatggact attaccactt
     2041 acaataatca gctgatgtcc aaaccagttt ctgttaaaac ctatcgggaa ttggtggaat
     2101 taatctatga accttttcac gctagagtgc gaaaactcta tgaaaatttc gaacgagagg
     2161 ggcgaactaa agtttttcat attgatgctc acagtatgcc ttcagtggga actgcagagc
     2221 atcgcgaccc gggcgagcgt cgggccgata ttgtagtgag cgattgtcac ggtcaaagct
     2281 gtgatcctca attccgcgat ctggtgattg cggcttatgt agttgcggga tttaaagtgg
     2341 cctataattg gccttacttt ggggggcggc tgagtgaaca ttatggtcgc cccgctcagc
     2401 ggcatcatgc gattcaagtg gaaatgaatc gggggctcta tatggacgaa accaccaaga
     2461 agttaaagtc tgcggaggct caaaaaattc aagccaaagt ggaattagca ctgagttata
     2521 tttgccaaca aatgctgaat ttacagagag ggtagaaggg taacggggag cgttgaggag
     2581 gataagatta gccctcgcac ctaagttaga ttaaatttct tccattatgt ctgataataa
     2641 taatgatgtt gttgatgccc tgaagcgcag gcttcgtcgt cttattaaat tcttggcttc
     2701 gttaaagctc gccgttgtgg ttattgctgc ccttgcttgt gttatcgcgg caggcactct
     2761 tattgagtct aaatacgacg ccacggctgc cagaaaaatg gtttatgaga ctttctggat
     2821 gtacagcgtg atgggagtgc tggcgacaaa tttaattgcc gtgatggtgg atcgttggcc
     2881 ttggaagcgg cgccataccc catttatttt tgcacacatt ggaattttaa ttttgatgtt
     2941 gggagctgtg ataactcaaa agtatggctt ggacggaagt gtgcgggtcg gcattggtga
     3001 gtctaatcaa tatgtgatcg tacctgaaac cgatattttg gtttacacct ctttcgatgg
     3061 ggatcgttac accaagctgg cggaacaaaa tgttgatttt tttaccacac ctccgacgcc
     3121 gcaaaagcct ttgatcgtca ctggctataa ctcagatatt aagttcactg cctacaaacc
     3181 ctacgtcatc ccctcccata aagtcacggt accgaaagag cttagcgggc gtcgcggctc
     3241 cgcacttcgt tttcaaattc aaagcgaaaa aattaaagtg aatgtggttg aatggatcgt
     3301 gcagcgtcgt cccaatgagg tggccaccca tgattttggt ccggctcagc ttcatttggg
     3361 ccttgcccct gcaaaagggc gaggtgccaa cgagatctat tttacgccgg attccgctaa
     3421 ggatggttta aaatatgtgg tctttaaaaa agactctgag gagcctgtaa aaacgggttt
     3481 tataaaagag ggtgatattt ttaacccagg ttggaaaatg ccgatcgaag tgcgagctct
     3541 tagatacttg ccggaggcgg tggaagagtg ggatcttcaa gatcgtgcga gccctacgcc
     3601 gctgacgact tcagcggtga agttagagtt taagggtcag gatcactggt tgcttttaaa
     3661 tgataccgtg aagcttttta cgcaagaggt tgtctatata gtcagttatg gaaatcgtcg
     3721 tttggatttg ggatttccaa tcagacttaa aaactttgaa atcggccgtt atcagggaac
     3781 tgcaaaggcc atggcttata agagtatggt ggaagttcct gatttggctg gtactcagga
     3841 gatttctatg aatgagcctt taaaatacag aggctttacg atttatcagg cgagttttca
     3901 ggaggatcca aaatctcaaa tgcccacggc ttccattttt tccgtgaatc aagatccggg
     3961 ccgatggtta aagtaccttg ggtgcttcat tatggttctg gggattatta tgcttttcta
     4021 ttttagaaag aaagcaaaga aggaaagtcc tgtatgagac gtttacagtt tattctggtt
     4081 cttttgttgt ccatgcttat ttacaaggat gtttttgcgg cccctggtga tgagatgcga
     4141 tacttgccgg tgcaagatgc ggggcgtatt aaaccctacg acaccttctc caaagaggtc
     4201 ctagagctca tctatggaaa gtccaccttt gaagatcatg gcgccccaga aattattttc
     4261 acttggatgc tcagtccgac agcttggcaa agtaaaaaaa tatttgaagt gcgcaatcat
     4321 gaggttttaa aggcgctgaa gcttcccatc gagcagcggt actttaccgg tgatgagctt
     4381 tttgcaaatg agcgtttttc ccttcttctt caggagcttc aggcaaagcg tgaatccaaa
     4441 gagaaattaa cgccctattt tcaagctctg cagcgtctgg aaaatcagtt aatggtattc
     4501 cgagaagtcg ccgggggaaa tatgctgcgt ttaattccgc cgaaagaggg aaataggtgg
     4561 ctttccgttg cggactttca aggtgaacag caggcggctt tcgggtaaat gacaaaggct
     4621 ttcattgagc atattggtgc gcttgccaaa ggctcaccag cggaacaagt cactcaagct
     4681 gcgttggcct tggatgccag cgtgcttaaa tttcgtgaac aagcaaaaag caacaatcct
     4741 gagctctaca gtcaagagtc aaaagtaaaa tatgaggttt tctataacca ttttcatcct
     4801 ttccgttggg cttatatctt atatatgctg gcatccgtgg tgattttgat tgtatggtca
     4861 atggataagc caggtgtcat gaagtttgcc tgggttttag cctgctcggg ctttgctttg
     4921 cacaccttcg ggtttggcat tcggatgtat ttaatggatc gagcgccagt aaccaatatg
     4981 tacgagaccg tggtgtggat gtcctgggga gccgtggtct tttcttcagt gcttgaaatc
     5041 atctatagat acaggtttat cttctttgcc ggcactttgg gtgccatggt gtcattgatt
     5101 attgcggatt ctgctccggc ggttttagat ccttctttgc aacctttaga agcggtgctt
     5161 cgaagcaact actggctcac tattcacgta atgaccatca ccatcagtta tgcagccttc
     5221 ttcttagctt tcgtcttagg cgatataggg cttatttatt acatccgagg cgagcaaagc
     5281 cataaagaaa aaattcaagc cattgccgtc tcagtttaca gagccatgca aatcggaatt
     5341 tgttttctag ctcctggaat tatcctcgga ggaatctggg cagactacag ttggggccgc
     5401 ttttggggtt gggatcccaa ggaaacctgg gctctgattg ctctgttggg ttatctcgcg
     5461 gtgcttcatg gtcgtctcgg tggttggttt aaaaattttg gaaccattgc tgcgggggtt
     5521 gttactttca gtctagtgat catggcttgg tatggagtta actttgttct cggcgcagga
     5581 cttcattctt atggtttcgg cgcgggggga gtagaatatg ttgctgcttt tattggcgct
     5641 cactttctcc ttgtgcttta tgcttccctt gtgcggagaa agtagtcagt aatttttttg
     5701 aattctcatc aagcttgcaa aaagagagta gacaactcct atattccacc tttaaatata
     5761 aaccatcaaa aaaatatgat gtgagcgacg ctggtattcg ctatttatct gatacttacg
     5821 agatacttat tttataccta ccatcgcgat catcagtgca atcatgaggc aaaggaagat
     5881 tatgcatcgg gtatttagaa cttttgcagc agtggggctt cttgcgttga gccttgcagg
     5941 gtgtaaattt cagccagggt ggggttacaa ccaaggatac gcgccagaac agccgcttcc
     6001 tttcagtcac gaaatccatg ttacaaaaaa tcaaatgcaa tgtcagtatt gccataatca
     6061 ggtggaacgt tcgaagcact caagtattcc agctctttcc acctgtatga actgccatct
     6121 tcaagtaaag accgacagcc ccttcattca aaaattgcgt gaagcctatg ataagggcgg
     6181 gtctatcgag tgggtgcgcg tgcatatgct tccagatcat gttcagttca atcactctgc
     6241 gcatattgct aaaggcgtga actgccaaac atgtcatggc gaagtcgaga aaatggctaa
     6301 aataactcaa gttcaggatc tttcaatggg ctggtgtgtt aattgtcacc gtcaaccaga
     6361 gaataaagct cctttgaact gtacaacttg tcaccactag agtttgtgga aggtatcatg
     6421 tctgaaaata atcacgaaca tatgcacgag cattctgagt ctgaattatc ggcggaatta
     6481 gcagccaaaa aggcggcgcg tccgcaaatc gaaagaaaca atacccattg gcttactctg
     6541 gaacattaca acaatgatcc tgagttcatg aagaaagctg aaactgaatt tcaatcttca
     6601 ccccttcgtg aaagcgaaag tgaaggcggg tgggctcgcc gcgagttctt gaaattgatg
     6661 ggcgcctctt tggctatgtc cgcagcgggc tgtatccgtc gtccagtgca aaaaattgtt
     6721 ccttataata agcagcctga agaagtgact ttaggtgtgg ctaattacta tgcttctact
     6781 tattttgatg gcaatgaagg tctgggtgtt ttggtaaaaa cacgtgaagg tcgccctata
     6841 aaaatcgagg gcaatcccaa gttcccaatg aataaaggag gcttgagcgt tcgcgcccag
     6901 tcttccatct tgagtctata tgatcctgaa agactgcaag gtcctaagca taatattttc
     6961 aatgaaaaac gcagcaacaa ggacaccgtt agcattaagt gggatggcct tgatgataaa
     7021 gtcgtggctc agcttaaaaa agggggcgct gttgtcttga ctggcaatgt tgcttctcct
     7081 tctacgcgag cggtgatttc tgattttgcc caagcgttta aagcggatca cgtctcttgg
     7141 gagcctttag cgaatgaaga aattcgcgaa ggtcaaagag cttcttacgg tgaagccgtg
     7201 gtccctcaat atcgttttga taaagcaaaa atgattgttt ccattgatgc ggattttttg
     7261 ggaacctggt tgaatccaac aacgtttgga gcgcagttcg ccgctggtcg cagagatcct
     7321 aaaaaattgt ctaaacttgt ctcttttgat tccacgtatt cattgaccgg aggaaatgca
     7381 gatattcgag ttaagatcaa gccgtcgcaa cagcttgatg tggtgatggg tttgatcaat
     7441 gttctcgttg tgaataaaaa cctttctgct tatgccaata atgagaaatt caaggacagc
     7501 cttgtctctt atgcaaatac agcgcaaaaa ctggggatcg agcctgcttt atttacgaaa
     7561 gtggcaacag atctttggga aaacaaaggt caatcccttg tggtcgctgg cggtttacaa
     7621 acattgacct ctcgctccgc tgaattacaa atcgctgtga atttcttgaa cagcctttta
     7681 gaaaatgaag gtaaaacagt tgacggtaaa aacggcaacc caggcctgaa agcgtcttat
     7741 gctgagcttg aaaaactcat caaagagatg aatgcgggta aagttaagac tttgatcatc
     7801 catcgcgtga atccgatgta tgctcttcct aaagaggcag gttttgccga agctcttaaa
     7861 aaagtagata tggttgttta taccggagac cgtatagatg agacgggcga gcatgcgact
     7921 tatctagcgc cggacaatca tccattggaa acttgggggg atgcggagtt tgcaaaagat
     7981 atttttgcaa tccaacaacc cgctatccgc cctctgtacg acacgcgttc attccaattg
     8041 tctcttatga catgggctta tcttgcgaat gtcggcccta agcgtttgat cacctttgaa
     8101 actttctacg attacttgag aaacttctgg aaagaagaaa ttcaacccaa agccgctaaa
     8161 ggtaaagcct ttgaagattt ctgggctgaa gttctgcaaa atggttatgt aggagatttg
     8221 gatcattcta ctgcagcccg ctccttcaag tctgaagcct ttgctgcgat taaacctgct
     8281 agcaaccgct caggttttga gttggcactt tatccaacgg ttcaacatgg cgatggttcc
     8341 agtgctaaca attcttggca gcaggaactc ccagatccag tgactaaaat tgtttgggac
     8401 aactatgttt gcgtttccat cgctacagca gagaaatacc acttaaaaca atccacagtg
     8461 gtagagctta aagtgggtga caaaaaagta gaacttcctg ttcatattca accgggcctg
     8521 catgatgatg ttttggctgt ggcggttggt tatggtcgca ccagagcggg tagagtcgct
     8581 gatggtgttg gccaaaatat gtttgaattt gcaagctttg ccgggggccc agtattcgcg
     8641 ggtcaggaag caagcttcac caagacgact aagaaatata atctggccag tgtcgctggt
     8701 aataactcca tggaaggtcg tatgatcgtg gcggaagcaa cgctcaaaga ctacatgaag
     8761 aacgaacatg caggagtcca caggcataaa acttggagta tctggtcagg ccatcagtac
     8821 aatggtcaca agtggggtat gtctgtcgat ttgaacactt gtacaggctg ttccgcatgt
     8881 atgacggctt gccaatctga aaataatatc tctgtggtgg gtaagaccta cgttcttcaa
     8941 ggccgtgaaa tgcactggat gcgcgtggac agatattatg tcggcaatcc cgcagacgca
     9001 ttggctgtgt tccagccggt gatgtgtcaa cattgtgaca atgctccatg tgaaaccgtg
     9061 tgcccggttc tggccactgt tcacagtgac gagggattga atgaaatggt ttacaatcgc
     9121 tgtgtaggaa ctcgttattg tgcaaataac tgtccttaca aagttcgccg tttcaactgg
     9181 ttcaattacg caaaactcat tgaaaaaccc atgcacatgg ccttgaatcc agacgtgacg
     9241 gttcgtcctc gcggggtcat ggaaaaatgt tcgttctgtg ttcagagaat taaagaagct
     9301 aagaataaag cgaaattgga ggatcgtaag ctgaaagatg gagatgtcaa aacagcttgc
     9361 caaatagctt gtccaaccca ggcgattatt tttggcgatt taaatgatcc agaaagccag
     9421 gtggctaaga tctttaaaga cgagcctcgc tcttatgcat tacttgaaga atggcatgcg
     9481 gctccgtcgg ttcgttatct ttctaaaatt cgcaacaatg ataaagaaac accagctgct
     9541 gagaaaggtg ggcactaatg atccatcgta acaagttggt ccttggtgac aaagacctga
     9601 aagatattac agaagatatc tgtgctccga tagaggctta tccatccaaa gggtggttag
     9661 gtcttttaac cggagccctg agtattttga ttttctatgt aatcattctt gggattgtga
     9721 ttggtaaagg gatcggtctt ctgggggtga atcatccgaa tgcctggggt acgatgatcg
     9781 tgaccttcgt cttctggatt ggtatcggcc atgccgggac tttgatttcg gccattctat
     9841 ttcttttccg tcaaaagtgg agaacctctg tagctcggac ggcagaggct atgacggttt
     9901 tcgccgtgat gacagcgggt ctcttccctt tgttgcatac aggtcgtcct tggttggatt
     9961 actggttgtt cccatatcca aatcaacgcg gtcctctttg ggtgaatttc cgttcgccgc
    10021 ttttgtggga cgtgtttgcg gtgtccactt atgcgactgt cagtatggtg ttctggtacg
    10081 tcggtttgat tcctgacttt gccaccatta aagaccgtgc aaaaaacaag atccgtcgca
    10141 cagtttacgg ggctttgtcc ttgggctgga gaggaacagc gaaaaattgg agccattatg
    10201 agatgcttta cttgcttctt gccggtctct caactccgct ggttctttca gtgcatacga
    10261 tcgtatcctt tgacttcgcg gtctctagtc ttccaggttg gcataccacg atcttcccgc
    10321 cttatttcgt agccggtgcg atcttctctg ggtttgcgat ggtggtgacg ctgatgactt
    10381 tggtgcgtat tggggtccca gaattcaaaa attacattac tatcgatcat atggaagtaa
    10441 tgaataaaat cattatgtcc acgggtttga tggttggtta cgcttatggt tctgagttct
    10501 ttatcgcttg gtattccgga aatccttatg aaaaatatgt gttttttgta agtcgtgctt
    10561 tcggacccta tgcctggtct tattggacga tgatcacctg caacgttttg atcccgcaag
    10621 tgttctggtt taagaaggct cgtcgctcta ttccaatcat gttcgtggtt tctattttcg
    10681 tgaatattgg tatgtggttt gagcggtttg tgatcactat cacttcactg catcgagatt
    10741 tcttgccggc aaactgggga aattacgcat ggtcattcta tgacggtgcc atccttttcg
    10801 gttcgtttgg gatgttctta acattgttct tgttgtactt gagatttttt ccggcgattt
    10861 cgattgctga gattaagcct gtattgccag taggacgtga aagcaaaggg ggcactaatg
    10921 gctaagcatg gacctggcgg catcgccgga atttggttag acgaacacac tgttgtaaag
    10981 gccgcggcca aaactcgtga aagcggtttt gtgaaattcg atgctattac tccttatccg
    11041 gtgcatggta tggaagaagc ctgtggtatt aagcgctcct ggattcccta tgttactttc
    11101 ggtgctggtg cagtggggct tctttctgga ctttggttga cttggtggac ttccgctgtg
    11161 gactggccca tcaacgtggg tggtaagccc tttttctcct taccagcgtt tattcctgtt
    11221 atctttgagc tcacgatttt atttgcagct ttgacttctg taggggcttt gttttacgta
    11281 acaaaaattc ctcgtttaga tcctccaaat attgaccctg accttacttc tcataaattt
    11341 gcgattttca tcccagaaaa tgatattggc tacgatgagt cgaagattga gcgctttttt
    11401 aaagaaaatg gcgcggttga agtgaagaaa atggcggagt attaagatga gatcacaaca
    11461 aattgagaaa atcatcctta tagcagcggc caccttggct ttttccgcct gcggagctaa
    11521 gggcgacaaa tctaacgtgg aattaatcca ggatatgatg gagtctccgg caattaaatc
    11581 tcaggagtat gacgaaagtt ctccgaacca ctctggcatg agaattcccc cagacaatac
    11641 agctcctgtt ggttttgctc cctataaatt taagggcaat ttggagggtg cggataaaaa
    11701 tgtaaaccct ctggctggaa atatgactcc ggatgttttg aaggtaggac aaaaatattt
    11761 tgaaacccac tgcaccgttt gccatgggca gacgggcgct ggaaatccag attcaaatgt
    11821 tgttaaactt atggcattaa aaccaccgtt gattttaagt gataaagtca aaggctggac
    11881 agatggacat atttatcatg tcatcagcga aggccagggt gtgatgggac cgtatgcctc
    11941 tcatatccca caagcttatc gttggcaggt tgttaactat attcgtttct tacaaaaaga
    12001 atctaagtag gttgtagata tggcagtatc aaatcattca aacgaattac acgttggcaa
    12061 attcgaagct tcgtcgaagc taaaaattac gagctacgta ctcatcgcta ttggtcttat
    12121 tactttcgct atggggctta tgaaggacca agaaagaata tgggccgctt atttggtctc
    12181 ttttttcttt ttctcttgtg tgggtttggg cggacttttt tggacctcac ttcagaattt
    12241 agtgaaagcg ggatggagtg tctctattcg ccgctatgct gaaggtttga gttctttcat
    12301 ccctgtgatg gtggttgcaa gctttgttct gattttgggt cttaaatatt tatatccttg
    12361 ggcggatcat gagaaagtgg ccgagagcgc gcttcttcaa agtaaatcca ctttcttaaa
    12421 taccaccttc ttaatcatcc gtcttgtggt gtttggacta gggatgttgg ctttccgtca
    12481 agtgattgta ggcaattcct tgaagcagga taagtctgga gatgagcaac tgacacataa
    12541 aaatg
//
LOCUS       DATBTE010000010        16375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_479074, whole genome shotgun sequence.
ACCESSION   DATBTE010000010 DATBTE010000000
VERSION     DATBTE010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 16375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16375
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479074"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1602)
                     /locus_tag="VIG33_00255"
     CDS             complement(190..1602)
                     /locus_tag="VIG33_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8269290.1"
                     /translation="MTNTLNTLIVDDETELRRSVISILKATIPDVEFVIDEACTGKEA
                     LDKVRSQAWDLVLMDVKMPEMNGLEALAAIKEHDPRTFVVLMTAHSNLQDAVTAIKDG
                     AYDYVEKPVNPQRLTEIVRTCLEARDLVSSLALSNPIFDDDIESEFVGSSQKMKEVFN
                     LIYRLCKVETTVLIRGENGTGKELVARAIHFNSPRKSGAFTAINCGAIPEALMESELF
                     GHEKGAFTGAVERKIGKFQVANNGTLFLDEIGELKPDMQVKLLRVLQEKKFTPVGGNR
                     EVKTNTRIIAATNRNLEKMMEDGSFREDLFYRLNVMPIFLPPLRDRTDDIPALVPHFI
                     KKFAKQHASGITSISPEALDILKSYRWPGNIRELENVIERAFIVENSNQITAESLPEN
                     VREVSKAHSMKRPPLGYSGPLDFDAFKDQMEKDFIISALKANNGRINQTVAQANIPKN
                     TLLRKIRKYGINVKEYSSEE"
     gene            complement(1599..3440)
                     /locus_tag="VIG33_00260"
     CDS             complement(1599..3440)
                     /locus_tag="VIG33_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8269291.1"
                     /translation="MKWVFRMVFRRRGFWLLTLLCWVIGIMALSSDEGNSYDQRFQLR
                     GDQFISPQIVLITIKQSDFTSIYEKRTNSMANVNELTDITDSFFWNKEIWLSLLKKIL
                     VQNPQSIGVTLYFGINVGDVNLSRAEEKIFKDPRITWSTSTNNFERLYEPLFTNDTHG
                     NIASNDLRRDEDGVIRHIFEQSDVLPHLVEKIAHKKFPASRTGITINYRGSGRIFTQY
                     SLAEVLNEEIPANAFTNKIILIGAETNSGPQYLTPVGALSRAETLAHITDDLLGDKWI
                     RQWHFGIYALLFLALTLIAVSLITQYPQSVALVFMLWLGLSIAAISAWIFDTFYIWLP
                     AYSPAILLLATWVIFIGYQANRNERLNFRLQQQQKSLEELEQLKNNFVSLISHDLKTP
                     IAKIQAIVNRLLNQHSSPELQEDLKSLRGSGDELNRYIQSILKVLRVESRDFKVNKEV
                     ADINEIIEEAIAQLRPLATEKNIRIQTELEPMFSVEFDTILIKEVLVNLIDNAIKYTP
                     RNGQISIYSNETEDSIFVKVKDNGEGIRPEDLDNVWGKFTRGKDQEMKSKGSGLGLYL
                     VKYFIELHGGKVDLQSKVGFGSTVSFTLPLSDDTSDNSSDNFQEVIS"
     gene            3548..4486
                     /locus_tag="VIG33_00265"
     CDS             3548..4486
                     /locus_tag="VIG33_00265"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEY8269292.1"
                     /translation="MQNFQGVEELPQEWRASVVSIGNFDGVHLGHQKLLRLAVQEGKT
                     LGVPIVVFTFYPHPVTVLYPERKIQRLFDSRDQREQLNRLGITHLIEQEFTHNFAQLS
                     AADFVQFYLIDKLKVKTLVVGYDFSFGSHRQGDIPYLQKICQQKNIKLVVVSAHEEDG
                     VIVSSSKIRAELKLGDLKKVYSFLGRNYYLRGIVHHGDARGRTLGVPTANMSPTVDFV
                     PRRGVYITQTHYQNKTYSSITNIGFNPTFHGESHPLIKTETHLFDFNEDIYGQEIQVD
                     LLQYLRDEKKFSGVQELLAQIHADIQQAKDFFHGRC"
     gene            4473..5351
                     /locus_tag="VIG33_00270"
     CDS             4473..5351
                     /locus_tag="VIG33_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269293.1"
                     /translation="MVDVNANPVSGKIFEFAETLSLAGLHMNHYFKNRNWRAECLSLP
                     GRELKDLKAWLPYVQEGALLLKMPLENSAEFLAHFPQLPTAVSWLEAADSLVLENGQW
                     WPRLYLCDTLRTLVIHRFRDLDISLNSYVIGANKTGRAVAAALAELGFSHVSLVDEDA
                     EKLDTEIKLLRRYLFGVQIEGILAQTLTIQEKPGSMMVNTLRMQSHDSILGDLSYFNF
                     MKAGGVVIDISECTPHNQLLEEAERANLKILSGLAVQAQYDVDMLVRLFPHQYITYED
                     YFESFGEALPSLTVSP"
     gene            5442..7154
                     /gene="pilB"
                     /locus_tag="VIG33_00275"
     CDS             5442..7154
                     /gene="pilB"
                     /locus_tag="VIG33_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEY8269294.1"
                     /translation="MSVLKIGEILVKQGLLRPDQLNQAHEEQKKSGGARLSQIIIGLG
                     FLKDNQILRAMEKYYQVPGVEVNTFEIDASVVSLVPREVCDKYTLIPLQKAGTTLVIA
                     FSDPSNIIVKEDLRFITRCRIQAVVGTEVAIASAIDKNYGGLISSKTLNSMSQESQDE
                     EFGALVGANVEIIDESGSGGEDAPIIKFVNAILSDAIKRRVSDIHFEPYEKRYRVRFR
                     IDGGLIEATAPPSGSAAAISSRIKIMSKLDIAEKRRPQDGRLKVRTSKGKEMDFRVSV
                     LPTLFGEKVVLRLLDKSNLQLDMTKLGFEEDDLKLFKQIINAPQGMVLITGPTGSGKT
                     TTIYSALYELNKFDVNICTAEDPVEFNLEGINQVQMNADINLTFSQALKSFLRQDPDI
                     VMVGEIRDLETAEIAFKAASTGHLVVSTLHTNDAPATIVRLTEMGVAPYIITATVNLI
                     VAQRLVGKICENCKIPIEVPAQTLINLGIHPDEVPEYKLFKGRGCATCNGSGIKGRAA
                     IYELMAMTEKVKEAILKGASAGQLRFLAREQGMRTLRRSALIKLKRGITTIEEVLNSS
                     VKDI"
     gene            7155..8201
                     /locus_tag="VIG33_00280"
     CDS             7155..8201
                     /locus_tag="VIG33_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269295.1"
                     /translation="MPSSNYFSEARKRKSQEILLVLGSEAKCRVQQSWVSLNSSPVLL
                     SEWNQVLKSVLKNEQLQRLEREGALQGCAQMSDGSRISYNIFQSHDCFKAHFCFEPLN
                     VFETELSLPTVFLETVSRCQGLILLAGPVHPYKTSTLAQTLYRLNKDRAFHGAIFSRQ
                     VFPGIPEEKASFVYQSTSVTAAAESGFFSGADVVVLHETMTAQSLAHAMDLCDEGKMV
                     VMTIASHSLMSAFHKCLELLSEKSNPHSLWRFADHLRMAISQHPVVSLSEDTILGFEM
                     LLNTPQMKTWLGQGQLASLEGLLQSHQENSGVLSLNQSLLQLLIRRRIDMKQAFLVTQ
                     DPDSLDQLLKKVGI"
     gene            8203..9432
                     /locus_tag="VIG33_00285"
     CDS             8203..9432
                     /locus_tag="VIG33_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY8269296.1"
                     /translation="MARFSYSAKNTAGQTVQGEIETSNENEARVRLRAQQLTPLRLVR
                     SSGGSRTSAKKSIGGFFAPKVKSKDLQIFTRQFSTLINAGIPVVDALKILSEGLRPGL
                     LRETAAKVKSSIENGKRLADSMMATSGVFDKLYVNMIQAGEEAGILDAILNRLAVYME
                     KSEKIKGQVKGAMVYPGVILIVAVLVISGILVFIIPKFNEFYSSAGKAPPALTQMVVA
                     LSNTLVHKWYLFIGGAIAIPYGIISYLDTADGKKTFDRFVLKVPVVGEVALKSAIARL
                     SRTLSTLLSSGVGLIDAIEIAARTSGNNVIEGVLIKSKESVIQGRSFSAPLSKEKVIP
                     EMVVQMIAIGEQSGTMDVMLGKIADFYEDEVETSVKAMTSLIEPMMMVFLGGIIAILV
                     IAMYLPIFSMGDIVGGG"
     gene            9439..11028
                     /locus_tag="VIG33_00290"
     CDS             9439..11028
                     /locus_tag="VIG33_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8269297.1"
                     /translation="MSTAMGSFKADNQNQKLIIAFSRVSLYLLILLVSQTAFFLQKSF
                     INWPLLAPFYMAICIALSFHGLYVHFMEWMAEKHVLLLVSFILDSLLLSLLITFSGLN
                     QSLFLFLHLVNILLAGLVLQSRGALTIALFSSIFFTLASFFGPEMKAMNYIFLLALNN
                     IAFFSVAGLSGYLSEQLQFMGVELTKTGLTLREIQELNKSIVSHMPSGLLTFTQAGTI
                     LQDNLSAQSIFGGKTLRDLNIFGFFPDLLKDTTQKRDYHWRNPEEAKILQVSVSSVFS
                     PVVEGELYIAQIEDMTRVKQLEYSLRQNEKMAAVGGLAAGIAHEIRNPLASISGSIEL
                     LSQTTANDDDRKLMKIILREINRLNNLITEFLEYSSPEKPPTTPVDLVSLIKEVMEGL
                     KFDGKLRQDVTAEVDLPETALILGRRDKLKQAFLNIVINAYQSMEKTSIARFEVTLRY
                     EMQSEKQSWKVTFKDTGCGMNESTRRRMFEPFHTTKSKGTGLGLAVTHKVLEGHGAQI
                     FVESEPEVGTSFVLIFAAVSV"
     gene            11092..12501
                     /locus_tag="VIG33_00295"
     CDS             11092..12501
                     /locus_tag="VIG33_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8269298.1"
                     /translation="MKSRILVVDDEESIREFLEIMLKKEGYEITLAEDGQKAKDLLAK
                     KSFDMIISDLQMPHVTGIELLKHVKETAPEIVFMMITAFGTTETAVEAMKMGAYDYIT
                     KPFKIDEVRLNIQNALRSRNLEVENRSLKKELVKEYSFQSMVGNSAPMHAIFDMITRV
                     SQTPTNVLVTGESGTGKEVVAKGIHYNGPLKDKPFVTINCGAIPENLMESEMFGHKKG
                     SFTGAVVDKAGLFEVANTGTLFLDEVGELPLSIQVKLLRAIQERVIRRVGATDDMKIE
                     VRIIAATNRNLEEMVQKGTFRQDLFYRLNVINIKTPSLRERRDDIPLLANHFLKKYNE
                     RLNKNIGGISTEAMEILKKYDYPGNVRELENLIERTVALEGGAMILPESLPPMVNTTS
                     GRKMASSNEIEIGEDGVDLDKVMGQIEKELLIKAIHSGGGVKKRAAKLLHISFRSMRY
                     RIEKYNLGIVGDDELSDEE"
     gene            complement(12519..13301)
                     /locus_tag="VIG33_00300"
     CDS             complement(12519..13301)
                     /locus_tag="VIG33_00300"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY8269299.1"
                     /translation="MKIIFAFLGSALFFLATAKAQSLNELKSQYEEKSPLSYQDLKPK
                     QVVEADASDKTPAEEQLAEMKAKELEEKKAQEEKAYLNTDHQFFQSLNITLDEDELDK
                     VFKEKQLAENIEKLKNTDTTVKTEITTTAPGLISLKEAEVVDEKRNLILKKISTNGQY
                     IRACILQFKKNQEFKGTAMTLAWEVDPSGKVVNTQMKVTDVENKEIKDCVLKTLAEWN
                     FSDAMKAQTKNSHIEYTYRFINAKKEAAAPKPLSQATATLQN"
     gene            complement(13445..14275)
                     /locus_tag="VIG33_00305"
     CDS             complement(13445..14275)
                     /locus_tag="VIG33_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY8269300.1"
                     /translation="MKTTLLFILTASFITLSCATTTQEGAVGINRKQYLSGVSSEQIL
                     AESEKSYAQTKQEAQKKGLLDKNPAQYQRVAAIARRLIPLTPIFRKDALSWPWESHVI
                     TSPELNAYCMPGGKIIFYSGLIEKLQLTDGEIAAIMGHEISHALREHGRERISEQMLQ
                     QGIAQGGLAVLVATGVIDPKYAGVASVGVSAFTTLFISLPHNRTQESEADTMGVELMA
                     RAGYDPHEALSLWNKMASQGGSKPPEMLSTHPTDSTRLQHIESLLPKVMPLYEQARRK
                     "
     gene            complement(14301..>16375)
                     /locus_tag="VIG33_00310"
     CDS             complement(14301..>16375)
                     /locus_tag="VIG33_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8269301.1"
                     /translation="FVCWIFLGSWTATLNILLAIPTSIMGAFIGLYFLNFTLNTFTLL
                     GLTLAIGIVVDDAIMVLENIFRYNEKGLDKIESAIIGAREITFAAIAATAAIIAIFLP
                     VVFMKGVIGKFFLQYGVTISLAVLISLLESLTITPMRCSSFVHTGERKTLLGRSFEKL
                     MEQGKIFYGKSLVVTLNHRWKILLTSLLFVVASFYLVGFLNKELTPVQDQSLFILRLM
                     TPIGSSLAFTNSKAQAVEQWLRQRPEIAHVYVAVGGLGTGGGSDTNTGMMFVTMKDKD
                     QRSLSQQQFMAVARKELAQIQDLKTFMLDLSSRGFSTGRGYPIEFALQGPDWDKLAEL
                     DESMKKSMRDSGLMVDVDSDYREGMPEIQILPDRTRAAFRGVSMDALGNTVNALIGGV
                     KNGQYTKNGHRYDIYVQLEKVPDPRKELSQLLIGNARNNLIPISQVSKIQQKLSLQQI
                     SRANRQRSITLYANLVPGASQQAALDFIKKKSKELPPTYFIEQSGAAKAFQESFQSLI
                     LAFALGLFVAYIVLASQFNSFLDPFAVLMALPFSFSGAFFALFLTNNTLNMYSMIGLL
                     LLMGIVKKNSILLIDFTKKVRDREKKNAAEALLVAGPIRLRPIIMTSFATIAAAIPSA
                     LGTGAGSETFRPMALTLIGGVFVSTLLTLYIVPITYSLLDRFQRQSHRKEVVKEAFEH
                     VDKTALER"
BASE COUNT         4319 a         3515 c         3940 g         4601 t
ORIGIN      
        1 aaacgttcaa gtttcgaacg ctgacatcac ggccccccac ttagattatt cacagggcaa
       61 tgtgactggc gcatctaatg acgtagtacc ttctggctct ttcgagatga gcattgatgt
      121 tgcgaattga tttcgtttaa aaaccagtcc ttttccgtca ccggaaaagg acctcttaaa
      181 gagcgagccc tattcctcgc tcgaatattc tttcacattg atcccatact tacggatctt
      241 ccgcagcagg gtgttcttcg gaatattcgc ctgagccacg gtttgattga tgcgtccgtt
      301 attggctttc aaggcgctga tgataaaatc tttttccatt tgatctttga aggcatcaaa
      361 atccagggga cccgaatagc ctagcggagg ccttttcatg gagtgagctt tgctgacttc
      421 acgaacattt tcgggaagac tttcggcggt gatttggttt gagttttcca cgatgaaggc
      481 gcgctcaatg acgttttcaa gttcacggat gtttccgggc cagcgatagg atttgagaat
      541 atccaaagcc tcaggggaaa tactggtgat gccactggcg tgctgcttgg cgaatttttt
      601 tataaaatgc ggaaccagtg ccgggatgtc atccgtgcgg tcgcgcagcg gcggcaagaa
      661 gatgggcatg acgttcaaac gatagaacaa atcttcacgg aaagaaccgt cctccatcat
      721 tttctctaga ttgcgattgg tggcggcgat aatacgagtg ttggttttca cctcgcggtt
      781 gccccccaca ggcgtgaact tcttttcttg caagacacgc aggagtttca cctgcatatc
      841 gggttttaat tccccgatct catccaaaaa caaggtcccg ttgttggcca cttgaaattt
      901 gccgattttt ctttccacgg ctcctgtgaa agcgcccttt tcgtgtccga aaagttcgct
      961 ttccatcaaa gcctcaggga tggctccgca gttaatcgcg gtgaaagcgc cacttttgcg
     1021 aggagaattg aagtgaatgg cccgagccac aagctccttg ccagttccat tttctccgcg
     1081 aatcaggacc gtggtttcca ctttgcaaag gcgataaatg aggttaaaga cctctttcat
     1141 tttttgcgaa ctgccgacaa attcactttc gatgtcatcg tcaaaaatcg gatttgaaag
     1201 ggccaagcta gaaaccaaat cacgggcttc caaacaggtg cgcacaattt cagtcaagcg
     1261 ctgtggattg acaggcttct ccacgtaatc ataggcgcca tcttttatcg ccgtaacggc
     1321 gtcttgcaaa ttactatggg cggtcatcaa gaccacaaag gtgcgggggt cgtgctcttt
     1381 aatggccgct agagcttcca gtccattcat ctctggcatt ttcacatcca tcaaaaccaa
     1441 atcccaggct tggctgcgaa ctttgtcgag ggcttctttg ccagtgcaag cttcatcaat
     1501 gacaaactcc acgtcaggaa tggtggcctt cagaatcgaa attactgatc ggcggagttc
     1561 tgtttcgtca tcaacgatca gagtatttaa agtattcgtc atgaaatcac ctcttgaaag
     1621 ttgtcgctag aattatcgct tgtatcatca cttaagggaa gggtaaatga cactgtgcta
     1681 ccaaagccga ccttgctctg gagatccact tttccaccgt ggagttcgat aaaatatttt
     1741 accagataca ggccgagtcc ggaacctttg gatttcattt cctgatcttt accccgggtg
     1801 aatttgcccc aaacgttgtc caagtcttcg gggcgaatcc cttctccgtt atcttttact
     1861 tttacaaaaa tagaatcttc agtttcgttg gagtaaatgg aaatttgtcc atttcgcgga
     1921 gtgtacttga tcgcattatc aataaggttc accagcactt ccttaatcag aatcgtatca
     1981 aattccaccg agaacatcgg ctccaactcg gtttgaatgc gaatattttt ttcggtggcc
     2041 agagggcgca actgtgctat cgcttcttcg atgatttcgt tgatatccgc aacttcctta
     2101 ttcaccttga agtcccgaga ctcaacccgg agaactttaa gaatggattg aatgtagcgg
     2161 ttgagctcat ccccgctgcc gcgaagagat ttcaaatctt cttgcaattc aggactggag
     2221 tgctgattga gcaagcgatt gacgatggcc tgaatttttg caatgggagt tttgagatca
     2281 tggctgatca gactgacaaa gttgtttttc agttgctcca attcttccaa agatttttgc
     2341 tgctgctgta agcggaagtt caagcgttca ttacgattgg cttggtatcc gataaagatt
     2401 acccaggtcg ccaaaagcaa aatggccggc gagtaagcgg ggagccaaat atagaaagta
     2461 tcaaaaatcc aggccgagat cgccgcaatc gaaagtccca gccacagcat aaataccagg
     2521 gctacggatt ggggatactg agtgattaat gaaaccgcga tcagggtcag tgctaaaaag
     2581 agcaaagcat aaatgccaaa atgccattgg cgaatccatt tgtcgccgag caagtcgtcg
     2641 gtgatatggg ccagggtttc ggcgcgcgac agagccccca ccggggtcag atactggggt
     2701 cctgaattgg tctctgctcc gattaaaata attttgttag taaaggcatt tgctggaatc
     2761 tcctcattga gaacttcagc cagactgtac tgagtgaaga ttcggccaga accacgataa
     2821 ttgatggtga tgccagtgcg cgaagcaggg aattttttat gggcaatttt ctccaccaag
     2881 tgaggtagca catcgctttg ttcaaagatg tggcgaatca ctccgtcttc gtcgcggcga
     2941 agatcattgc tggcaatatt tccgtgagtg tcgttggtga acaaaggttc gtagagacgt
     3001 tcaaagttat ttgtacttgt tgaccaagtg atgcggggat ctttaaagat tttttcttca
     3061 gcgcggctga ggttaacgtc tccgacattg atgccgaagt aaagggtcac gccaatagac
     3121 tggggatttt gcaccagaat ttttttaagc agtgagagcc aaatctcttt gttccaaaaa
     3181 aagctatccg tgatgtcagt cagttcgttg acgttcgcca ttgagttcgt gcgtttctcg
     3241 tagatcgagg taaagtcgga ttgcttgatg gtgatcaaaa caatttgtgg ggaaataaat
     3301 tgatcccccc gcagttgaaa gcgctggtca taggagtttc cttcatcact ggataaggcc
     3361 atgattccga tcacccagca gaggagggtg agaagccaaa atccacgccg ccgaaaaacc
     3421 attcgaaaaa cccatttcat aagggttgca attttgttaa aaataagagc tgaattcacg
     3481 caatgcagcg taacaaattt tgtgaaaaat tcaagaatta aaggagctta tagagtatta
     3541 tcctcgtatg cagaattttc agggtgtaga ggaactcccc caggagtgga gagcctccgt
     3601 agtgagcatt gggaactttg atggcgtcca tctcggtcat caaaaactat tacgtctagc
     3661 ggtccaagag ggaaaaacac ttggggtgcc gattgtggtc tttacttttt acccacatcc
     3721 ggtgacggtc ttatacccgg agcggaaaat tcaacgtctt tttgactccc gcgatcagcg
     3781 cgagcaactg aaccgtctcg ggatcaccca tttgattgaa caggagttca cccacaattt
     3841 tgcccagctc tcggccgctg attttgtgca gttttatctg atcgataaac tcaaagtaaa
     3901 aaccttggtg gtgggctatg atttttcttt tggttcgcat cgccaagggg atattcccta
     3961 tttgcaaaaa atctgtcagc agaaaaatat taaactggta gtggtttcgg cacatgagga
     4021 agatggcgtc attgtctcct cttcaaagat tcgtgccgag ctcaaactgg gggatttaaa
     4081 aaaagtgtac agcttcctgg ggcgtaatta ctatctgcgt ggcatcgttc atcatgggga
     4141 tgcgcggggg cgcactcttg gagtgccgac cgcgaacatg agcccgaccg tggattttgt
     4201 gcctcgtcgc ggagtttaca ttacgcaaac tcattatcag aataaaacgt attcctcgat
     4261 taccaacatt ggatttaatc ccacttttca cggcgagagt catcctctga ttaaaaccga
     4321 gacccatctt tttgatttta atgaagacat ttatgggcaa gagattcaag tggatctttt
     4381 gcaatatctg agagatgaaa aaaaattctc cggcgttcag gaactgttag cgcagattca
     4441 tgccgacatt cagcaggcga aggacttctt ccatggtcga tgttaatgcg aatccggtga
     4501 gcggtaaaat tttcgaattc gcagagactc tgtctttggc cggtctgcat atgaatcact
     4561 attttaaaaa tcgcaattgg cgggcagaat gcctttcact gccaggacgc gagttgaaag
     4621 atctaaaagc atggctgccc tacgtgcagg aaggggcttt gttgttaaaa atgcccctag
     4681 agaattctgc agagtttctc gcacattttc ctcagcttcc gacagcggtg agttggctcg
     4741 aggccgcgga ttctttagtc ctagaaaatg gtcagtggtg gccgcgtttg tacttatgcg
     4801 acactcttcg tacgctggtt atacatcgtt ttcgggattt ggatattagc ctgaattcct
     4861 atgtgatcgg cgcaaataaa accggacggg cggtggcggc cgctttagct gagttgggct
     4921 tttcccatgt gagtctcgtg gatgaggacg cggagaagtt ggacacagag ataaaacttc
     4981 ttcggcgcta tttgtttggg gtgcaaattg aaggtatttt ggctcaaact ctgacgattc
     5041 aagaaaaacc gggatccatg atggtgaaca ctttgcggat gcagagtcac gattcgattc
     5101 ttggagacct ctcttatttt aattttatga aagctggggg tgtggttatc gatatttccg
     5161 aatgcactcc tcacaatcag ctcttagaag aagccgaacg agcgaactta aaaattctct
     5221 ctggcctggc ggttcaagct caatatgatg tggatatgct ggtaaggctt tttcctcatc
     5281 aatatatcac ttatgaagat tatttcgaga gcttcgggga agctctgcct tctttaacag
     5341 tttcaccttg agctttactt tgagcttctc tttaattttg cccttgaaaa cccttcttcc
     5401 gtctagttac aaaaaatgtc accccaagct agaatagcaa tatgtcggta cttaaaattg
     5461 gcgaaattct ggttaaacag ggactgctgc ggcctgatca attaaatcag gctcacgaag
     5521 aacaaaaaaa atcaggcggg gcccgtctga gtcaaattat tataggcctc ggatttctta
     5581 aagataatca aattctgcgc gcaatggaaa agtactatca ggttccagga gtagaggtaa
     5641 atacttttga aatcgatgcc agtgtcgtga gcttggtccc acgggaagtt tgcgacaagt
     5701 acactttgat tcctcttcaa aaagccggaa ctacattggt gatcgctttt tctgatccca
     5761 gcaatatcat cgttaaagag gatttgcgtt tcatcactcg ttgccggatt caagccgtcg
     5821 tcggtactga agtcgccatc gcttcggcga ttgataaaaa ttacggcggc ttaatcagtt
     5881 ctaaaacgct caattccatg agccaggaat ctcaagatga agaatttggg gctttggtcg
     5941 gggcaaatgt tgagatcatt gatgaaagcg gcagtggtgg tgaagatgcg ccgattatta
     6001 aatttgtaaa cgctatatta tctgatgcga ttaaacgccg tgtttctgat atccattttg
     6061 aaccctatga aaaacggtat cgcgtgcgct ttcgaattga cggtggcttg atcgaagcaa
     6121 cggctccacc ttcgggttca gcggcggcga tttcctcacg gattaaaatc atgtctaagt
     6181 tggacattgc cgagaaacgc cgtccacagg atggacgttt gaaagtccgt acttccaaag
     6241 gaaaggaaat ggattttcgc gtgagtgtcc tgccgactct ctttggtgag aaagtggtcc
     6301 ttcgcctttt agataaatcg aatctgcaat tggatatgac caaattggga tttgaagaag
     6361 atgacttgaa actttttaag caaatcatca atgctccgca agggatggtt ttaatcaccg
     6421 gcccaacggg ttccggtaaa accaccacta tttactcggc actttatgaa ttgaataaat
     6481 tcgacgtgaa catctgcacg gcggaagatc cggtggaatt taacttagaa ggcatcaatc
     6541 aggtgcagat gaatgcggat attaatctga ccttctctca ggcgttgaag tcttttcttc
     6601 gtcaagatcc tgatattgtg atggtggggg agatccggga tctggaaacg gcggagatcg
     6661 cctttaaagc agcctcaaca ggtcatttgg tggtcagtac tctgcatacc aatgatgccc
     6721 ccgcgaccat tgttcgtctg accgagatgg gagtggctcc ctacattatc actgcgacag
     6781 tgaatcttat cgttgctcag cgcctcgtcg gaaaaatctg tgaaaactgt aagattccga
     6841 ttgaagttcc cgcgcagacc ttaataaatc tggggattca cccggatgag gttcccgaat
     6901 acaaactgtt caaggggcgt ggctgtgcga cttgcaatgg ttcaggaatt aagggccgtg
     6961 ccgcaatcta tgagttgatg gcaatgacgg aaaaagtcaa agaggccatt ttaaagggtg
     7021 cttccgcagg ccaattgcgt tttctcgcgc gcgagcaggg gatgcggact ctgcgcagga
     7081 gtgctttgat taaactaaaa cgcggaatca cgacaatcga agaagtgctt aattcatctg
     7141 tgaaggacat atagatgccc agctcaaact atttttctga agcaagaaaa agaaagtccc
     7201 aggagatcct cttggtttta ggaagcgaag ctaaatgtcg cgttcagcaa agctgggtga
     7261 gtttaaattc atctcccgta cttttgagtg agtggaatca agtgttgaaa tccgtcttga
     7321 agaatgagca attgcaacgt cttgaacgtg aaggcgcttt gcaggggtgc gcccagatgt
     7381 cggatggctc tcgcatctct tataatattt ttcaaagtca tgattgtttt aaagctcatt
     7441 tttgttttga acccttgaat gtttttgaaa cagaactcag tctccctact gtgttcctgg
     7501 aaacagtgag ccgttgccaa ggtttgattc ttctggcagg cccggtgcat ccttataaaa
     7561 ccagcacttt ggcgcagact ctttatcgct tgaataaaga ccgggcgttt catggagcga
     7621 tcttttccag acaagttttc ccaggaatcc ctgaggagaa agccagtttt gtctatcaaa
     7681 gcacttctgt caccgctgcc gccgaaagcg gttttttctc cggggcggat gtggtggttc
     7741 tccatgaaac aatgaccgct cagtcattgg ctcatgcgat ggatctgtgt gatgagggca
     7801 aaatggtggt gatgacaatc gcttcgcatt ctttgatgag tgctttccat aaatgtctgg
     7861 aactgctttc tgaaaaatcc aatccccatt ccctctggcg atttgcggat catttaagga
     7921 tggcaatttc tcaacatccg gtggtttcct tatcagagga caccatattg ggttttgaaa
     7981 tgctgctgaa tactccacag atgaaaactt ggttggggca aggacagttg gcgtccttgg
     8041 aaggtcttct acaatcccat caagagaaca gcggagtact cagtttgaat cagtcattat
     8101 tgcagctttt gattcgtcgt cgcattgata tgaaacaagc ttttctagtg actcaagatc
     8161 cagatagttt ggaccaactt cttaaaaagg taggcatcta atatggctcg tttttcttac
     8221 agcgccaaaa atacggctgg gcaaacagtt caaggggaaa ttgaaacttc caatgagaac
     8281 gaagctcgag tgcgcttgcg tgcacagcaa ttgacgcctt tacgcttggt gcgaagcagt
     8341 ggggggtctc gaacgtcagc taaaaaaagc atcggtggct tctttgctcc taaagtcaaa
     8401 agtaaagatc ttcaaatctt tacccgccag ttctctacgc tgatcaatgc cggtattccg
     8461 gtggtggatg ccctgaaaat tctttcagag ggtttgcgcc cgggccttct tcgcgagact
     8521 gccgctaaag taaaatcttc cattgaaaac ggcaaacgtt tggcggactc catgatggct
     8581 acttctggag tttttgacaa actttatgtg aacatgattc aagcgggcga ggaggccggt
     8641 atcttagacg cgatcttaaa tcggcttgcg gtctatatgg aaaaatccga gaagatcaaa
     8701 ggtcaagtta agggcgcgat ggtttatcct ggggttatct tgatcgtggc cgttctggtc
     8761 atctcgggaa ttctggtttt catcattcct aaatttaatg agttctatag ttctgcggga
     8821 aaagcgcctc ccgctttgac gcaaatggtg gtggcactga gtaatacact ggttcacaag
     8881 tggtatttat ttatcggtgg agctatcgcg attccttatg gaattatatc ttatttggat
     8941 accgctgatg gtaagaaaac cttcgaccga tttgttttga aagtcccggt tgtgggtgaa
     9001 gtggcgttga aatccgccat cgctcggctg agtcggactt tgtccacttt gctttcttct
     9061 ggcgtgggtt tgattgatgc gattgaaatc gcggctcgaa cctcgggcaa taacgttatt
     9121 gaaggagttt tgattaagag caaggaatcc gtgattcaag gtcggagttt ttcagcaccc
     9181 ctttctaaag aaaaagtaat tcccgaaatg gtggtgcaaa tgatcgccat cggagagcag
     9241 tccggcacca tggacgtgat gttgggtaag atcgctgact tttatgagga tgaagtggag
     9301 acctcagtca aggccatgac ttctttgatc gagcctatga tgatggtttt cttggggggc
     9361 attatcgcta ttttggttat cgctatgtat ctaccaatct ttagtatggg tgatatcgtt
     9421 ggtggaggat agtgcttcat gagcacagcc atgggctcat ttaaagctga taatcaaaat
     9481 caaaagctga tcatcgcatt ttcgcgggtc agcctttatt tgcttatttt attggtaagt
     9541 cagactgcgt tttttctaca aaaaagtttt attaattggc ctttattagc ccccttttac
     9601 atggcgattt gcatcgctct ttcttttcat gggctttatg ttcactttat ggagtggatg
     9661 gcggaaaaac atgttctgct tttggttagc ttcattctgg attcactgct gttgtcctta
     9721 ttaatcacct tcagtggttt aaatcagtcg ctttttttat ttttgcattt ggtgaatatt
     9781 ttgctcgcag gcttggttct tcaatctcga ggagccttaa ccatcgcact ttttagcagt
     9841 atttttttta cgctcgcaag tttttttggt cccgaaatga aggcaatgaa ttatattttt
     9901 cttttagctc tgaataatat cgctttcttt tccgtcgcgg gcctgtcggg ctatttgagc
     9961 gagcaactgc agttcatggg cgtggaactc acaaaaacgg gtttaaccct gcgtgaaatt
    10021 caggaactca ataagtccat tgtcagccat atgccttcgg gcttgctcac tttcactcag
    10081 gcgggcacca ttcttcagga caacctttca gcccaaagta tttttggcgg gaaaaccctt
    10141 cgggatctaa atatttttgg attttttccg gatttattaa aagatacaac ccagaagaga
    10201 gactatcatt ggaggaatcc tgaggaagcg aagatcctgc aagtgagcgt ctcctctgta
    10261 ttcagccccg tggtcgaagg ggaattgtat atcgcacaga ttgaagatat gacccgggtc
    10321 aaacagttgg agtattcgct ccgtcagaat gaaaagatgg ctgctgtcgg gggcttagcg
    10381 gcggggattg ctcatgagat tcgcaatcca cttgcaagta tcagtggtag tattgaactt
    10441 ttaagtcaaa cgaccgctaa tgatgatgat cgcaagctta tgaagatcat tcttcgtgaa
    10501 attaatcgtc ttaataatct catcacggag tttttggagt attcgagccc cgagaaaccg
    10561 ccgacgaccc ccgtggatct ggtgagcctc attaaagagg tcatggaagg gttaaaattt
    10621 gacggtaaac tccgtcaaga cgtgacggca gaggtcgacc ttccagaaac agccttaatc
    10681 ttgggtcgtc gggataagtt gaagcaggcc tttttaaata ttgtgattaa tgcttatcag
    10741 tcgatggaga agacttccat tgctaggttt gaagtgactc ttcgctatga aatgcaaagt
    10801 gaaaagcaaa gttggaaagt cacgttcaaa gatacagggt gtggcatgaa tgagtccaca
    10861 cgtcgacgta tgtttgaacc gttccacacc acgaagagca aagggacagg ccttggactg
    10921 gctgtcactc ataaagttct cgaaggacat ggcgcacaga tctttgtcga gagcgagccg
    10981 gaggtcggga ccagtttcgt cctgattttc gctgctgtta gtgtttaggt tgaataaaag
    11041 attttgaatt tctcgctgtg acaagcgaga ataaatttat taggagtatt catgaaatcc
    11101 cgtattttgg ttgtagacga tgaagagtcc attcgcgaat tcctcgaaat catgctcaaa
    11161 aaagaaggtt atgaaataac cctcgctgaa gatggccaga aagccaaaga tcttctagct
    11221 aagaaaagtt ttgatatgat catctcagat ctgcagatgc cacatgtgac gggaattgag
    11281 cttctgaaac atgttaaaga aaccgcacca gaaatagttt tcatgatgat cacggctttc
    11341 ggcactaccg aaacagcagt ggaagccatg aaaatgggcg cctatgacta tatcactaag
    11401 ccgtttaaga ttgatgaagt gcgcttgaat attcaaaacg ccctccgctc tcgaaacctg
    11461 gaagttgaaa atagatctct taaaaaagag ctggttaaag aatacagttt tcaaagtatg
    11521 gtgggaaact ccgctccaat gcatgcgatt tttgacatga taacgcgtgt ttcgcaaact
    11581 ccgacaaatg tgcttgtcac cggcgaatct ggaacgggta aggaagtggt cgccaagggt
    11641 atccactaca atggaccttt gaaagacaag ccctttgtca cgatcaactg tggtgccatt
    11701 ccagagaact tgatggaatc cgaaatgttc ggtcataaga agggttcctt tacaggtgcc
    11761 gttgttgata aggcgggcct ttttgaggtg gcgaacacag gaactttatt tctggatgaa
    11821 gtgggtgaat tgcccctttc aattcaagta aaacttctgc gggcgattca agagcgtgtc
    11881 atccggcggg tgggtgccac ggatgatatg aaaatagaag tgcgcatcat cgcggcgacc
    11941 aacagaaatc tggaagagat ggttcaaaag ggaactttcc gccaggactt gttctatcgc
    12001 ttgaatgtca tcaatatcaa aactccaagc cttcgtgaaa gacgggatga tattccgctc
    12061 ttggctaacc attttttgaa gaagtacaac gaacgcctca ataaaaacat tggcgggatt
    12121 tctacggaag cgatggagat ccttaaaaaa tatgattacc cgggaaatgt gcgtgaattg
    12181 gaaaatctta tagagcgcac ggtcgccctt gagggcggcg ccatgatctt accagagagt
    12241 cttccgccca tggttaacac cacttcaggc cgcaagatgg cctcttccaa tgaaatcgaa
    12301 attggtgagg acggcgtgga tcttgataaa gtcatggggc aaatcgagaa agaattgttg
    12361 atcaaagcga tccactccgg cggcggcgta aaaaaacgtg ccgcgaaact cctgcatatt
    12421 tctttccgct ctatgcgcta tcgtatcgag aaatacaatc ttggtattgt tggcgatgac
    12481 gagttgtcgg acgaagaata agcggtcctt ttgcgttctc agttctgtaa agtggcggtc
    12541 gcttggctaa gtggtttagg ggcggctgct tcttttttcg cattaataaa gcggtaagtg
    12601 tattcaatat gtgaattttt ggtttgtgct ttcatggcat cgctaaaatt ccattcggct
    12661 aatgttttaa gcacgcagtc cttgatttct ttattttcca catcagtgac tttcatctgt
    12721 gtattgacca ctttaccact aggatcaact tcccaggcta gagtcatcgc tgttccttta
    12781 aattcttgat tctttttgaa ctgtaaaata caagcgcgaa tatattgtcc attagtggaa
    12841 atcttcttta agattaaatt tctcttttca tcgaccactt cggcttcctt cagacttatc
    12901 aaacccggag ccgtggttgt gatttctgtt ttaacagttg tgtctgtatt tttaagtttc
    12961 tctatatttt ccgcgagttg tttttcttta aaaaccttat ccaactcatc ttcatcaagc
    13021 gtaatgttga gagattggaa gaactgatga tcggtattaa gatacgcttt ttcttcctgc
    13081 gccttttttt cctcaagttc cttggctttc atttccgcca attgttcctc tgccggcgtc
    13141 ttatcagaag cgtctgcttc gaccacttgt ttcggtttaa gatcttgata acttaacgga
    13201 gatttttcct catattgact tttgagttcg ttgagactct gagctttggc tgtagctaag
    13261 aaaaaaagag ccgacccgag aaaagcgaaa atgattttca tatctccccc tcaaaaaaac
    13321 aatccaactt cagtttaaca tggcgagttt ttgaattgct agaggacaaa acgaaaaaat
    13381 gctgtctatg atttgatcac aggccaaaaa aagaccagga gtttgacagc ggcgtttcca
    13441 gaatttattt ccttcttgcc tgttcataaa ggggcattac tttcggcaat aaggattcga
    13501 tatgttgaag gcgagtggag tccgtcggat gtgtactcag catttctggg ggcttagatc
    13561 ctccttgaga cgccatttta ttccacaagc tcaaggcttc gtgggggtca tagcccgctc
    13621 gtgccatcaa ttccactccc atggtatcgg cttcggactc ttgagtacga ttgtgaggca
    13681 acgaaatgaa aagagtggta aaggccgata ctcctacaga ggccacgcct gcgtattttg
    13741 gatctatgac cccagtggcg accagaacgg cgaggccacc ctgggcaatt ccttgttgta
    13801 gcatttgctc cgagatgcgt tcgcggccat gttcgcgcag agcatgggag atctcatggc
    13861 ccatgatcgc ggcgatttct ccgtccgtaa gttgcaattt ctcgatgagg ccgctgtaaa
    13921 aaataatttt accacccggc atgcagtagg cattgagttc aggactggta atcacatgag
    13981 attcccaagg ccatgaaaga gcgtcttttc taaaaatggg tgtcaaggga atgagacgtc
    14041 tggcgatggc ggcaactctc tggtattgag cgggattctt atcaagaagg cctttctttt
    14101 gagcctcttg tttggtctgc gcataacttt tttcagactc cgcaagaatc tgctctgaag
    14161 agactccaga aagatattgt ttacggttga tgcccaccgc gccttcttga gtggtggtgg
    14221 cgcaggacag agtaataaag cttgcggtta aaataaaaag tagagttgtt ttcataaacg
    14281 tatttttatc tccgaatatt ttatctttca agggctgttt tatccacgtg ttcaaaggcc
    14341 tctttaacta cttctttgcg gtgactttgc cgctgaaagc ggtctaagag agagtaagtg
    14401 ataggcacga tataaagcgt taaaagcgtg gaaacaaaaa ccccgccaat cagagtcaag
    14461 gccatagggc ggaacgtttc agagccagcg cctgttccta aggcggaggg aatggccgca
    14521 gcgatagtgg caaaagaagt cataatgatc ggccttaggc gaataggtcc tgcgactaaa
    14581 agagcctcgg ccgcattctt tttttcgcga tcacgtacct ttttggtaaa atcaatcagc
    14641 agtatggaat ttttcttaac gatccccatg agcaacagta aaccaatcat ggaatacata
    14701 ttgagagtgt tattggtgag aaagagtgcg aaaaaagcac cgctgaaact aaaaggcaga
    14761 gccataagta ctgcaaatgg atccaagaaa gaattgaatt ggctggccag aacgatataa
    14821 gctacaaaca gtcccagagc aaaagccaaa atgagacttt ggaaagattc ttgaaaggct
    14881 ttggcggctc ctgattgctc gataaagtaa gtcgggggaa gttcctttga cttttttttg
    14941 atgaaatcca aagccgcctg ttgcgaggct cccggaacca aattcgcata aagcgtgatg
    15001 gacctctgac ggttggcgcg ggagatctgt tgcaaactca acttttgttg tatcttactg
    15061 acttgagaaa tcggaatgag attgtttctg gcgttgccaa taagcagttg cgagagttct
    15121 tttcgagggt caggaacttt ctctaactga acatagatat catagcgatg accgttctta
    15181 gtgtactggc catttttcac tccgccaatg agggcattca ctgtatttcc gagggcatcc
    15241 atgctgactc cacgaaaagc ggcccgggtg cgatcaggca ggatctgaat ttcaggcatc
    15301 ccttcacgat agtctgagtc gacatccacc ataagacccg aatcacgcat ggattttttc
    15361 atggactcat caagttcagc caacttatcc caatccggtc cctgcaaagc aaattcaatc
    15421 ggatagcctc gaccggtgga aaatcctcga gaagataaat ccagcataaa ggttttcaaa
    15481 tcttgtatct gggccagctc tttgcgggcc acggccataa attgttgttg cgaaagactt
    15541 ctttggtctt tatctttcat cgttacaaac atcattccgg tattggtatc actaccgcca
    15601 cctgtgccta agccaccgac ggcgacatag acatgggcaa tctctggcct ttgccgcagc
    15661 cattgctcca cggcctgggc cttggagttc gtaaaggcca acgaagagcc aatgggagtc
    15721 atgaggcgga ggataaataa gctttgatcc tggaccggtg tcagttcctt attgagaaat
    15781 cctacaagat aaaaagaggc gacgacaaaa agcaaagagg ttaataagat tttccatcga
    15841 tgattcagcg tcaccaccag gctttttcca tagaaaattt ttccctgctc catgagtttt
    15901 tcaaaacttc ggcccaggag tgttttgcgc tctcccgtat ggacgaagct cgaacaacgc
    15961 atcggagtaa tagttaaaga ttctaaaaga gatattagaa ccgccaggga aatggtcact
    16021 ccgtattgga gaaaaaattt tccaatgacc cctttcatga aaacgactgg caaaaaaatg
    16081 gcgatgatgg cggcggtggc ggcaatggcg gcgaaggtga tttctcgcgc gccaatgatg
    16141 gcggactcaa ttttgtccaa acctttttca ttgtaacgaa agatattctc taacaccatg
    16201 atcgcatcat ccaccacgat cccaatagcg agagtcagac ctaaaagggt aaaagtattt
    16261 aaagtaaagt tcaaaaaata gagccctatg aaggcgccca tgatcgaagt cggaatagcg
    16321 aggaggatat ttaatgtcgc tgtccaactt cctaagaaaa tccaacagac gaaag
//
LOCUS       DATBTE010000011        20838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1367480, whole genome shotgun sequence.
ACCESSION   DATBTE010000011 DATBTE010000000
VERSION     DATBTE010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 20838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20838
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367480"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2024)
                     /locus_tag="VIG33_00315"
     CDS             complement(<1..2024)
                     /locus_tag="VIG33_00315"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY8269302.1"
                     /translation="MKIISHRKAPTPSKYPQTFNHHGDERTDSYFWLKEVQHPETKPY
                     LEAENFHTEEFFESYKGIEQNLFEEMKARVVKDDSTPPAKNEDYYYYTRMEGDKEQAF
                     YCRRKGSPDGPEEVLLDCNELAKGQKSFSLGIYEISPRHDVLAYASDTNGAEVYTLYF
                     KNLNTGKLFPEIIVQAYHSGAWAKDNKTFYYVTQDENQRPNKLWRHTLGTLITQDEVL
                     FEEGDNQYFISCEISESETFILLHRHSHATTEYYYIDAEDPKAKLQLFSALEQNVQYD
                     ITHRGDYFYILTNKEAQTFKLCRTPLNKTSKENWETLIVATPERTLKSLSAFKDFMLV
                     AATEQALQTLYKFDYDTFQMEPIQFPDPIYQVSFGANYEFATDWVRLNYNSLTQPNQT
                     YDLNIKTQEKIVRKIQKIPSGYNPEEYFSERIWAISHDGIKVPVSLVYKKNLRKQGPQ
                     PTLLYAYGSYGISMPTAFNLARISLLDRGYIYAIAHIRGGSDMGRQWYEEGKFLKKKN
                     TFYDFIACAEHLIKHNYAEPKTLSIMGGSAGGMLMGAVINLRPDLFNTALAIVPFVDV
                     LNTMLDSSLPLTQMEYQEWGNPEVSQEYYEYIKSYSPYDNVKAQNYPNIYIKAGLNDP
                     RVTYWEPAKWCAKLRELKTDNHVLLLETDMESGHAGASGRFEYLKEVARQ"
     gene            complement(2087..2833)
                     /locus_tag="VIG33_00320"
     CDS             complement(2087..2833)
                     /locus_tag="VIG33_00320"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY8269303.1"
                     /translation="MYPIIRLAEGLEIPSFFLNISLVISLSLFWIIRRGDLYHLPKKN
                     ILDLSLLLMGSSLVGARFMHILYENFSYYQEHPLRVFYLWQGGFVFYGGLVLAFVTTL
                     VYLKWLQTDKKSHYFDAFAPVLAFAYGFGRIGCFLAGCCYGKTCEYPWAMNGRHPAQL
                     YAFFLEVGIISLLLGIEKVPLSKRPRFLNRSGDIFVLWLALHSLGRLMMESFRDDFRG
                     DQILGQSVSTILSLLLFLISSVSLYSRKPT"
     gene            2934..4364
                     /locus_tag="VIG33_00325"
     CDS             2934..4364
                     /locus_tag="VIG33_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8269304.1"
                     /translation="MRLHPVEVAQELKTFSFFKSFAEDLLLQVSTMMVAKSFKKGEFI
                     LRQGQPNHSLFFLRTGTVEIILSGEVVAELSRIGEVFGEMSVISENPATTSVRAVADV
                     ECFTVKAADFEHVHPKEKDHFYALLYRLYSSILVERLIKTNEKARLFEIANRELQRVQ
                     ADLQAVGDKKVLLVESDKKQQVIARMAVGGTGVRLDIANNKDEAEGFIGEKKYDAILC
                     EEAFLDILVQAHKNKISPHLVLLTNPDVTANIQTIKGLPFVDNIMSRDPEDRSFTVRS
                     LLTTLTKVLNRNIFGIEKYLSWGADIHVKVVKTSRERAALKDEMIAHFKQSGIRASTL
                     DRVYSVAEEILMNAIYDAPADAMGQALFNHLPRKTEINLEKNQESTLRYAFDGTMIAL
                     SVEDPFGTLTKETIIAYLESCYQGKAGSLNANKGGAGRGLHQIIENSDLTIFNVKKGV
                     KTEVISLFSLEMGRKEHNPSFHYFFV"
     gene            4548..5459
                     /locus_tag="VIG33_00330"
     CDS             4548..5459
                     /locus_tag="VIG33_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8269305.1"
                     /translation="MEKFVSAVWAVDPTDSNLRPGTEALTEIRHFLGGGFSRVHPVYI
                     YSDETQVMQNSQIQSQVQEYLKPLHLGETGEIETIFTASSKRADWAKKILQIAHSQKA
                     QLILLTSHGRSTLGSFVLGSFAHEILRKSDVPVLFISPSSIKTAKTEKVLFATDFSKE
                     SKAAFQDFLQLIKGKTSEVILFHSINLPILSMSVAQGGGLSMSLPDSYLQNQKEWADS
                     EIHHWLEEVPKSDMKIQFHPLVEDSLTSPAAAIEKIAERENVGLIGLTSHAGPVTRLI
                     FGSVTQDLLSSQKFNLWVCGPAQTTIR"
     gene            complement(5462..5965)
                     /gene="lspA"
                     /locus_tag="VIG33_00335"
     CDS             complement(5462..5965)
                     /gene="lspA"
                     /locus_tag="VIG33_00335"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY8269306.1"
                     /translation="MKKKYLYLAVITGLLVAADQLVKMYIHTQFHLGESVVVIPNFFN
                     LTYVRNFGAAFGFLAEAHPTFREIFFLAMPPIALIIILTIMRGVRDDDTKQIIALSAI
                     FGGAIGNYVDRLRFRYVIDFLDFHLNNKYSWPAFNIADSAIVCGVCLLLLMMVFEAKQ
                     KKPEEAV"
     gene            complement(5962..8742)
                     /gene="ileS"
                     /locus_tag="VIG33_00340"
     CDS             complement(5962..8742)
                     /gene="ileS"
                     /locus_tag="VIG33_00340"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEY8269307.1"
                     /translation="MSSTLPDYKSTVRLPLTDFPMKGNLPAKEPEILKQWDDKNIYHK
                     MVAKNAGKKPFVFPDGPPYANGSIHLGHALNKNLKDIIIKYKNMKGSPTVFIPGWDCH
                     GLPIEHKVLKDLAAKKLVKTDQEILALCRAEAQTWVNHQRSQFRRLGILADWDKPYLT
                     MSADYEAEEVREFARAFAKGNIYRGEKPVYWNWTLKTALAEAEVEYHNHKSPSIYVKF
                     NITDKATLEKVGNPSKPTSLVIWTTTPWTLPANLGISLHPDFEYSVYGVGCENLIIAK
                     PLKEFFEKDTGLNLTEGKIFKGKDLEHGKARHPFISRDSLILSGDHVTADAGTGAVHT
                     APGHGVDDYKVGLKYNLGLLSPVDESGKFTEEVPEYVGINIFKANPLIIERLKDSGHL
                     IMMKEIEHSYPHCWRTKTPLIYRTTPQWFMGIDLEHSQIRQRTLKEIEKVQFIPEWGR
                     ARFQAMMENRPDWCVSRQRIWGVPIPILACKATGEPFADVNVMMKIADIIEKQGGIEA
                     YYQVSADEIVGDTWKNSEAAKKDPKFGSAGFRHGRDILDVWFDSGICHAAVQKRREGM
                     GFPADIYLEGSDQHRGWFNTSMLSSMATNGQAPFKTLLTHGFVVDSQGRKQSKSLGNV
                     IDPNEVTAKSGAEIIRLWTAYEDYGKDVSCGKEELDRVTETYRRIRNTMRFLLGAVSD
                     FDPAKDKIAYENLTEIDQWALHVLYELTEKVTQAYDSYEFYKVYHLLNNFFTVDLSSI
                     YLDVLKDRLYTWKADGVPRRAAQTVIYTITDYLVRIMAPILSFLAEEVYSYVKGPKAE
                     SVFLLDFPESPMKWNNPSLSLKFVGLLKVRGDVQKVLENLRAQKVIGASLEAAVTIKA
                     EGELLKDLKSLKDLREFFIVSKAEVMMGSYEVLAEKAPGEKCVRCWVYSTEISHEPKT
                     LGVCPKCVEALT"
     gene            complement(8846..9376)
                     /locus_tag="VIG33_00345"
     CDS             complement(8846..9376)
                     /locus_tag="VIG33_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269308.1"
                     /translation="MTMNPLPPQAYTKDTLTKAYMWLVHQNASIRELATTPDILCSLF
                     LKAQRSGDESLDAPSIQNFKKELKSLAGMMGELEGKSPTSRMPHHAQYVTPTFQPPLP
                     IQPMESVQQPLQPQQPKQVNIQNNQVSMDMLSQLDAKSKAMISEVREQLNLSCDLEAL
                     RLLITLGYGKAKTLCP"
     gene            complement(9588..10676)
                     /locus_tag="VIG33_00350"
     CDS             complement(9588..10676)
                     /locus_tag="VIG33_00350"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY8269309.1"
                     /translation="MKAILALLSGLTAFASAHANTLNYDFRGDWQSYTYNTAAGLPDS
                     TRFYLKTGRLDFKGNLNDRFSYQLRWGFYKPAVDTGITNAKRDSLNSSVEYAYVTDKM
                     SDLFALSLGKVNTEIGGLEGATAGPDLYLVSPNYQHTAVKTLSGANIGINQSGASNIL
                     YMTGAKGDFNFLDQHIYVMVLNNIADSADPAGNFNQNRGLMGLTWKGSFFDKALTGLA
                     SYHEVSPQSATGDTGNKHNFISAGVKYDTSSWVGSLEYTTTTYKDGASGNKDTLTSEI
                     AKFGYKLDQWTPRLELFSSEEEIGIGTTTGTNKFMGYGVVVEYKPTAENFRYHLAYNT
                     ISAKPISGDTQTRTEIVLGARLLGDFLK"
     gene            complement(10814..12304)
                     /locus_tag="VIG33_00355"
     CDS             complement(10814..12304)
                     /locus_tag="VIG33_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8269310.1"
                     /translation="MLRVLVVDDDQGLRLAVKTALISTNRFEVEEAFDGLNAIEKIKA
                     AEKQFDVVILDVDMPRLNGLEALRLIKEHDPGIIVLMITAHATLDHAVQAVKDGAYNY
                     LSKPVSGDELLTLVDKAVSAHTMISNIAASAPVLVEAGRKIIGNTQQMQKVFNIIHRL
                     AKVDTPVLIRGASGSGKELVAKAIHYNSARKDEKFVAINCSAIPENLFESELFGHEKG
                     SFTGADQRKIGKFQFAEGGTLFLDEVGDMPVMMQVKILRVLQEKVFTPVGSNREISTN
                     VRIVAATNRPLEEMIKLGTFREDLFYRLNVVPIFLPALADRKEDIENLINIFIKKFNA
                     AQGRRISGVAADALSALKKYQWPGNIRELENVIEHAFVLETNNIITLASLPETVLAAV
                     GVNLINAPSVPLVVAEIEEDFDALDNDPLDLEEEAEGAVAIVSSTNESLDFNAQKEAF
                     EKEFIIKALKTFKGRINQTALHANIPKKTLLRKIEKYGIVAKDYSI"
     gene            complement(12297..14174)
                     /locus_tag="VIG33_00360"
     CDS             complement(12297..14174)
                     /locus_tag="VIG33_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8269311.1"
                     /translation="MALPPSLTKTTGFFKGGVLYSVLRLVLACAMGIFISQLKLDSWE
                     AGLYDLRIRLLPEPSPSGHIEIIMTTSRTVEDLKGLPRAKEQLEMLKNLGKEGPLAVL
                     YTQNLDELQGTLEEKKTFAKEAQKIKNLYMISQDLEMKGEEGKLKLSEPLSSIELFAG
                     PRTNDGDVLAQDKVTRRMIITYQDRQMVHPFIAGLVNPVIKDGNNIRGLFKFFDSEQV
                     YINYIKANTYPRTSFEDVMDGRFAPGRFKDKIVLIGNDHGGAIRDYAKTPFSSDAKAM
                     TLVELNANMFDTLIRNKAPIQASPWLNILVTILTSILTVHVLFTTKPIRSLIILALTG
                     ITLVVLGTVGFWPGGYWIKMAHPFLAIFLCYYFFIPYRLIIENRRSWEYYEKNKLLSQ
                     VEELKGNFISMMSHDLKTPIARIQGMTDVILKDPQPLSSQQREALDTIRQSDEDLLKF
                     INAILNYGKIESQGVELHMQSRDVNALLKDVICKHEFLAKVKRIPIEFECEPLFPIQV
                     DPELMKQVFSNLIENAIKYSPEDSHVRVQSEEKDGKVIVRVEDRGPGIPQDEVNNIFM
                     KFFRSKNAKSSPIKGSGLGLYLAKYFTELHHGRIFVESTYGKGSIFTVELPLEQGRSH
                     A"
     gene            complement(14176..14337)
                     /locus_tag="VIG33_00365"
     CDS             complement(14176..14337)
                     /locus_tag="VIG33_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269312.1"
                     /translation="MKFLTLIKEKIMEKVDKKHVKKLKPKTTQTANFGHGNGSNPCPP
                     VGGDLITEN"
     gene            complement(14382..15662)
                     /locus_tag="VIG33_00370"
     CDS             complement(14382..15662)
                     /locus_tag="VIG33_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY8269313.1"
                     /translation="MELRRYKDILALKSEEGTLLAFHARNLEVAQISEDTWRNMEPGS
                     VQKECEALEELQSWEIENNSEVKTSKIDSTIRFLTLNVTQICNLHCNYCAAGGDGTFG
                     DAIAKISLEKTLPQLKFFLDRLPPKVSFNISFLGGEPLLYADALALIATYVAEVAPGR
                     KVNYTVITNGTLLTDKNVDILAAMKATVTISLDGPPEINDVVRPTKNGTSSTAMIVAG
                     LEKLLARKSEFSHIMIHGVFDSHNMELEKAYEFYSHFAVDSYDFSFSVSENNPRATVE
                     YMEQMTKLAAAAFAKGGEGALRKIRGFEQYFQALDHQQRTENHCGAGKSFLMLDARNR
                     IYTCPWDVNDPKEQVGQGEELNEKALAAYQKPLLEVNHCENCWARFLCGGGCMFAHKH
                     ASGDKNIKNSQFCERTRFMVALTLIYYERCRSTA"
     gene            complement(16237..17931)
                     /locus_tag="VIG33_00375"
     CDS             complement(16237..17931)
                     /locus_tag="VIG33_00375"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEY8269314.1"
                     /translation="MGSSAVKKVLQELVFPYWTRSEDRWKARGNLLLMLVLILGMVYS
                     LVLLNRWNKAFYDALQNLNKEEFLSQLRQFFIIALSYSLIMAYKYYVLQSLAVSWRKW
                     LTEKNVTHWLSNQNFYYWQIKKNSEDNPDQRLSEDIHELTDLSLEISEKILREFITFI
                     SFIGILWGLSGSLKFSDINGYDIEIHRYLVWVCLLYAIVGTWIVHKIGSPLSKINFMQ
                     QKLEADFRYLLIRLRENSESIALLKGERAEKQKLSAKFFQVVGNFKELISCQKKLIMT
                     TNFYGQLAYIFPFVVASPKVFTREITLGQLFQISSAFGQVQGSVSVFVDMYTKLARLH
                     SVMIRLSGFLHSLEENHELRHATVSRSVQTTVGAYEIENLNVMTPQHQSLFNALSLRL
                     TPGKRVLITAPSGKGKTTLLRSMNGIWPYLAGQVRIPKECHRLVLTQKAYLPIGNLRS
                     ALAYPMAGENFNDQDFQKVLTLTKLAYLIPDLDKETSWSLSLSSGEQQRIAIARIFLQ
                     KPEIVFLDEATSALEKKTEKFLYETLIASFPQMIVVSFSHEEDALKEFHQECLVLN"
     gene            18006..19280
                     /locus_tag="VIG33_00380"
     CDS             18006..19280
                     /locus_tag="VIG33_00380"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEY8269315.1"
                     /translation="MSSKYVVRGLLSLFLGIGTQVLLGREARAAFANYNSILIGDQAA
                     GMGGAYTAMANDASAMAYYNPAGLAFLEGHTFSAAVGIYKKFDTTFGKEEDFTKAPLR
                     MNQGFFRAIPSSTGSVIKKGDYSLAFSIVVPDFDNYKGDLRAGSDNTTTLSYVDESLW
                     VGGAVAKKISETESLGLTMYYTARSYSRSLNDRSFPTVTEAILFNSEKTLVENAVVAV
                     LGYHLNLSENWSFGASLRTPNLSVKGMGSYFSSQVTTNPYSVTSVNLPDERTNVRIPG
                     KLAIGIAYRDVDDFTISADATLYQGLEYDDFNNQSFTTHFIHRQTWNLAAGAEKKVMP
                     WLKVRAGVFTNFSSHPTPDPSLNVSQPDKIDQMGFSANAVFVSGDKIDYTFGGYYTGG
                     RGKSIQQINQNAEEVIVTQHVFTMLVGTAFSF"
     gene            19385..20353
                     /locus_tag="VIG33_00385"
     CDS             19385..20353
                     /locus_tag="VIG33_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8269316.1"
                     /translation="MKFWGVRGSLPSSPSPAEWTNHIEGLLRGFFNAGLRDATQVSKY
                     IDSLGASHLGGYGAATTSVEVKSGKNRIIIDGGSGIRSLSEQIMSGKAGASKGPFHIF
                     MTHFHWDHIIGLPFFTPHFVPGVQIHYYAVQPEMEKLIRGIFTKPYFPVPFESLASKI
                     SFHVLEPRKTVEINDMKITPYKLDHPDPCWGFRVACGDKAYAHCVDTEGTRVSREELA
                     EDLPLYQGIDLMYYDAQYTFPELAEKANWGHSAAQVGLDLAFREGIKHVLFAHHDPGA
                     TIQQVQELKRQTLEYYDWRLTTAKENAEALNAVQWDFAYEGLTVKI"
     gene            complement(20371..>20838)
                     /locus_tag="VIG33_00390"
     CDS             complement(20371..>20838)
                     /locus_tag="VIG33_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HEY8269317.1"
                     /translation="EEVYHFTPRAEQVQDPVAVASLVGMMKQTIVSGTAKYVSASGFT
                     RPAAGKTGTTSDSRDVWFSGFTPYRTTIVWIGYDTSTMNKLTGASGAVPIWTDFMKHA
                     TIEDPVEDFAWPEGTVKVNLDENKLQEMEIPTKDPTNVELIFRKGTEPGLFRF"
BASE COUNT         5760 a         4507 c         4601 g         5970 t
ORIGIN      
        1 aactggcggg ccacttcctt cagatattca aatcgtccgg aagctccagc atggccggat
       61 tccatatctg tttccaaaag taagacatgg ttatccgttt tcaattcgcg gagttttgca
      121 caccatttcg ctggctccca ataggtcact cggggatcat tcaatccggc tttaatgtaa
      181 atatttggat aattttgcgc cttcacattg tcgtaaggtg aatagctttt aatatattca
      241 tagtactcct gcgagacttc tggatttccc cattcttgat attccatctg ggtgagtgga
      301 agactcgagt ccaacattgt atttaacaca tccacaaaag ggacaatcgc caaggctgta
      361 ttgaagaggt cagggcgcaa attgatcact gctcccatga gcatcccgcc ggcgctgccg
      421 cccattatcg agagagtttt gggttcagca tagttatgtt taatgaggtg ttccgcacaa
      481 gcgatgaagt cataaaaagt atttttcttt ttcaagaact taccttcttc gtaccattgg
      541 cgacccatgt cgctaccgcc acggatatga gcgatggcat agatatagcc gcggtcaagc
      601 aagctaatcc gagctaaatt gaaggccgtt ggcatgctta ttccgtagga accataagca
      661 taaagtaagg tgggttgggg accttgtttg cgaagatttt ttttatacac caaagaaacc
      721 ggtactttga tcccatcatg ggaaatcgcc caaattcttt ctgaaaaata ctcttcagga
      781 ttataacctg aggggatctt ctgaatctta cgcacgattt tttcttgagt cttgatattg
      841 agatcgtaag tctgattcgg ctgggttaaa gagttgtaat tcaatctcac ccagtccgta
      901 gcaaattcgt agttcgcacc gaagctgact tgatagatag gatccggaaa ctgaatgggt
      961 tccatctgaa aagtgtcata gtcgaacttg taaagagtct gtaaagcctg ttctgtagcg
     1021 gccaccagca taaaatcttt aaacgcggaa agcgatttca aggttctttc cggtgttgcc
     1081 acaatcaaag tctcccaatt ttctttggaa gtcttattaa gaggcgttcg gcagagctta
     1141 aaagtctggg cttctttatt agtcaggata taaaaataat caccccgatg ggtgatgtcg
     1201 tactggacat tttgttccaa ggcagaaaat aactgaagtt tggctttggg gtcctcagcg
     1261 tcgatataat aatattcagt ggtcgcatgg ctatgacgat ggaggagtat aaaagtttcg
     1321 ctctcagaaa tttcacagga aataaaatat tggttatctc cttcttcgaa gagaacctca
     1381 tcttgggtaa tcaaagtgcc caaagtatgg cgccataact tgttgggtct ttgattttca
     1441 tcttgggtca catagtagaa agttttatta tctttagccc aagcgccgct gtggtaagct
     1501 tgaacgataa tttccggaaa aagtttcccc gtgtttaagt tcttgaagta aagagtatag
     1561 acctctgcac cgttagtatc agaggcatag gccagaacat cgtgacgagg actgatttcg
     1621 tagattccca aagaaaaact cttttgtcct ttagcaagtt cattgcagtc caggaggact
     1681 tcctcaggac catcagggga tcccttgcgg cgacagtaaa aagcctgctc tttgtctcct
     1741 tccattcgcg tgtaataata gtagtcttca ttttttgcgg gtggagtcga gtcgtcttta
     1801 acaacgcggg ctttcatctc ctcaaagaga ttttgttcaa ttcccttata gctttcaaag
     1861 aattcttccg tatgaaaatt ttcggcttct aagtaaggtt ttgtttcggg atgttgaacc
     1921 tctttaagcc aaaagtagga gtctgttctt tcatcgccat gatggttgaa agtttgagga
     1981 tatttggaag gagtgggggc tttacgatga gaaattattt tcatagactt gcaaacatcc
     2041 tccttgaacg ggttttaatc aagaaaatat ccttaaaaaa caaaaactat gtgggcttcc
     2101 tgctgtataa agacacggag gaaatcaaaa acaataagag gcttaaaatt gtggaaacag
     2161 attggccgag aatctgatct ccacgaaaat cgtctcggaa actttccatc atgagccgcc
     2221 ctagggaatg gagagccagc cataaaacaa atatatctcc agaacggttt agaaatcgcg
     2281 gtcgttttga aagaggtact ttctcaattc ctaaaagaag cgaaattatg ccgacttcca
     2341 aaaaaaaggc gtagagttgt gcggggtgac gtccattcat tgcccagggg tattcgcaag
     2401 tttttccata gcaacatccc gctaaaaaac atcctattcg accgaaaccg taagcgaagg
     2461 ccaggactgg ggcgaaggcg tcaaaatagt gcgatttctt atctgtttga agccacttca
     2521 aatagactaa agttgttacg aaagcgagaa ccagtccgcc gtaaaagaca aagccccctt
     2581 gccaaagata aaaaactctt aggggatgtt cctggtaata agagaagttt tcatagagaa
     2641 tatgcataaa ccgagcccca accaaagaag aacccatgag taaaaggcta agatctaaaa
     2701 tatttttttt cggtaaatga taaagatctc cgcggcgaat tatccaaaat agactgagag
     2761 atattaccaa gcttatatta agaaagaagc ttggaatttc tagaccttcg gccaagcgaa
     2821 taataggata cactgaaact ccttgatgga ccttggactt aattttcaga caattctaaa
     2881 tttattcaag tgctaactgg tcaacaaaga aatgaaaatt tataatagtt tacatgagat
     2941 tacatcccgt agaagtcgcg caggagctta agaccttttc tttttttaag tcttttgctg
     3001 aagatcttct tttgcaagta tcaacgatga tggtggctaa gtcgtttaaa aaaggtgaat
     3061 ttatacttcg ccaaggccaa cccaatcact cgcttttttt tcttcgtacg ggaaccgtgg
     3121 aaattattct atcaggcgaa gttgtcgctg aactgagccg gattggtgaa gtcttcggag
     3181 aaatgagtgt tattagtgaa aatccggcca cgacttcggt gcgggccgtt gcggatgtgg
     3241 aatgtttcac tgtaaaggcc gctgactttg agcacgttca tccaaaagag aaagaccatt
     3301 tctatgctct cctttaccgc ctctactctt ctatcctggt ggaacgttta attaaaacca
     3361 acgaaaaggc ccggttgttt gagattgcca atcgtgaatt gcagcgggtt caagcggatt
     3421 tgcaggcggt gggcgataaa aaagtcctgc ttgttgaatc agataaaaaa caacaggtta
     3481 ttgcgcgtat ggcggtaggg ggaacgggag ttcggcttga tatcgctaat aataaagatg
     3541 aagcggaagg ctttataggc gaaaaaaaat acgatgcaat tctttgtgaa gaagcttttt
     3601 tagatatttt agttcaggct cataagaata aaatcagccc gcacttagtc ttgctaacga
     3661 atcctgatgt caccgctaat attcaaacta taaaaggcct tccttttgta gataatatta
     3721 tgtcgcgcga tccagaggat cgtagtttca cagtaaggag tctgctgacg actttaacca
     3781 aagttctaaa taggaatata ttcggaattg aaaaatatct ttcctggggt gcggatatcc
     3841 acgtaaaggt cgtaaaaacc agtcgtgaaa gagccgcgct gaaggatgaa atgattgctc
     3901 actttaaaca aagtggcatt cgcgcttcca ccctggaccg agtttattca gtagcagaag
     3961 agattttaat gaatgctatt tacgatgccc ctgcggatgc aatgggccaa gcacttttta
     4021 accatctgcc acgtaaaaca gaaattaatc tcgaaaaaaa tcaagaatca actttgcgtt
     4081 atgctttcga tgggaccatg atcgctttgt cggttgaaga tcctttcggg accctgacaa
     4141 aagaaaccat tattgcgtat ttggaaagtt gctatcaagg aaaagccgga tctttgaatg
     4201 ccaacaaagg cggtgcgggt cggggcctgc atcaaattat cgagaattca gatctaacta
     4261 tttttaatgt taaaaagggt gtgaaaactg aagtcataag cttgttcagt ttggaaatgg
     4321 gaagaaaaga gcacaatcct tcatttcatt acttttttgt ctaaaataaa aatctagcgc
     4381 ggcctgggac accttgtgct gggaatgggg caagttgtct caagaatttc atttatatca
     4441 cgcaaattcg tccgcatggt aattacagca atggcttata ttttgcactt ccttttgagg
     4501 aactcaattt tcactgggga aaaatgcctg agaggtggct cagtaatatg gaaaaattcg
     4561 tttcagctgt ttgggccgta gatcccaccg atagtaattt gcgtcctgga accgaagcat
     4621 tgactgaaat tcgccatttt ttgggcggcg gattttcgcg agttcatcct gtttatattt
     4681 attcggatga aacccaagtt atgcaaaata gccaaataca aagccaggtc caggagtatt
     4741 tgaagcccct tcatttggga gagactggcg agatagagac catttttacc gcttctagta
     4801 aaagagccga ttgggctaaa aagattttgc aaatagctca cagtcaaaaa gcacagctga
     4861 ttctcttaac ttcccatgga agatcaaccc tagggagttt tgtgttaggc agttttgctc
     4921 atgaaatcct gcgaaagtcc gatgtgccgg tactgtttat cagtccctcc agtattaaga
     4981 cggcaaagac tgaaaaagtt ttatttgcca cggatttctc taaagagtct aaagcggctt
     5041 ttcaagattt tttacaactc ataaaaggca aaacttccga ggtcatcctc tttcactcta
     5101 ttaatttacc tatcctttct atgagtgtgg ctcaaggagg agggctttcg atgtctttac
     5161 ctgactctta tcttcaaaat caaaaagaat gggcggacag cgaaatccat cattggcttg
     5221 aagaggtgcc taagagcgat atgaaaattc aatttcatcc tctggttgaa gactctttaa
     5281 cgtcccctgc ggcggccatc gaaaagattg ccgaaagaga aaatgtggga ctgatcggtt
     5341 taacttccca tgccgggccg gtgactcgac tgatttttgg aagtgtgacc caagatcttc
     5401 tgtcatcgca aaaattcaat ctgtgggttt gcgggccagc tcaaaccaca atacgctgaa
     5461 ttcatacagc ctcttccggt tttttctgct tggcttcaaa gaccatcata aggagcaaca
     5521 aacaaactcc gcaaacgatg gcactatccg cgatattaaa cgcaggccag ctgtatttat
     5581 tattgaggtg aaaatctaaa aagtcgatca cgtagcgaaa acgcagacga tcgacataat
     5641 ttccaatggc tccaccaaaa atcgcggaaa gagctatgat ttgtttggta tcgtcgtctc
     5701 ttactccacg cataatagtc aggataatga tgagagcaat agggggcatt gctaaaaaga
     5761 atatttcccg gaacgtcggg tgagcttccg ctaagaaacc aaaggccgcc ccaaagtttc
     5821 gaacgtaggt cagattgaag aagttaggga taactacaac ggattcaccg agatgaaatt
     5881 gagtgtgaat atacattttc actaactgat ccgcagcgac caagagaccg gtaataacag
     5941 ctaaatacaa atatttcttt ttcatgtgag tgcctctaca catttcggac aaacccctaa
     6001 agtcttaggt tcgtgactga tttcagttga ataaacccaa caacgcacac atttttcgcc
     6061 aggggctttc tcagccagga cttcatagga acccatcatt acctccgctt tagagacaat
     6121 gaaaaattca cgcaagtctt tcaagctctt caagtccttc aaaagctccc cttctgcctt
     6181 aatagtcacc gccgcttcca ggctagctcc aatgaccttt tgagcccgca ggttttctaa
     6241 aactttctgc acatcccccc gcactttcaa gagacctacg aatttcaggc tcaaagaagg
     6301 attattccac ttcataggag attcagggaa atcgagtaag aaaacgctct ctgcttttgg
     6361 gccctttaca tagctgtaaa cttcttcagc taagaaagac aaaataggag ccatgatacg
     6421 caccaaataa tccgtgatgg tgtagatcac cgtctgggcc gcgcggcgag gaacaccatc
     6481 tgccttccag gtgtacaggc gatcttttag aacatccaga taaatggacg acaaatctac
     6541 ggtgaaaaaa ttattgagca agtgatacac tttgtagaac tcataagagt cataagcttg
     6601 ggtcactttt tcagtcagtt catagagaac atgtaaagcc cactggtcaa tttccgtgag
     6661 attttcgtaa gctattttat ctttggctgg atcaaaatca ctgaccgccc ccaaaaggaa
     6721 gcgcatggta ttgcggatgc ggcgataggt ctctgtcact cgatccagct cttctttacc
     6781 acagctcaca tctttcccgt aatcttcgta agctgtccaa aggcgaatga tttccgcccc
     6841 tgattttgca gtgacctcgt ttggatcaat gacgttgccc aaagatttac tttgtttacg
     6901 tccttgtgaa tccaccacga aaccatgggt caataaggtc ttaaacggag cctgtccgtt
     6961 cgtagccatc gaggaaagca ttgaagtatt gaaccaacca cgatgttgat cactgccctc
     7021 aagatagata tccgctggga agcccatgcc ttcacggcgt ttttgtacag ccgcatggca
     7081 gattccagag tcaaaccata catccaagat gtcacggccg tggcggaagc ctgcggaacc
     7141 aaacttagga tctttttttg cagcctcgga attcttccac gtatcgccga cgatctcatc
     7201 agccgaaact tggtagtaag cttcaatgcc gccttgcttt tcaatgatat cagcgatttt
     7261 catcatgaca ttcacatcag caaagggttc gcccgtggct ttacaagcaa ggatagggat
     7321 tggaactccc cagatgcgtt gacggctcac gcaccaatca gggcggtttt ccatcatagc
     7381 ttgaaaacgg gctcgacccc attcagggat gaattgcact ttttcaattt ctttcaaagt
     7441 tctttgacgg atttgagagt gctcaagatc aatgcccatg aaccactgtg gagtcgtccg
     7501 ataaattaaa ggagttttcg ttctccagca gtgcggatag ctgtgctcga tttctttcat
     7561 catgatgagg tggcccgagt ctttcagacg ctcaatgatc aaaggatttg ctttaaagat
     7621 attgatgccc acgtattccg gaacttcttc ggtgaatttt ccagattcat caacgggact
     7681 caataaaccc agattatatt taagacccac tttatagtca tccacgccat ggcctggagc
     7741 tgtatgaacc gcccccgtac ccgcatcagc agtgacgtga tccccactca gaatcaatga
     7801 atcccgagag ataaaaggat ggcgggcttt gccatgttcg aggtcttttc ctttaaagat
     7861 ttttccctca gtcaggttga ggcctgtgtc tttttcgaag aactctttca acggttttgc
     7921 gatgatgaga ttctcacaac ctacaccgta aacagagtac tcaaaatcag ggtgcaaaga
     7981 gatccctaag ttagcaggca aggtccaagg agtggtcgtc cagatcacca ggcttgtagg
     8041 tttacttggg ttgcctactt tttccagggt ggctttatca gtaatgttga atttcacata
     8101 gatcgaagga cttttgtgat tatggtattc cacttcggct tccgccaaag ctgtttttaa
     8161 ggtccagttc cagtaaacag gtttttcacc acgatagata ttgcccttag caaaagcccg
     8221 ggcaaattca cgaacctcct cagcttcata atcagcagac atggtcaaat aaggtttatc
     8281 ccaatctgcc aggatgccta aacgacggaa ttgactgcgt tgatgattca cccaggtctg
     8341 agcctcagca cggcataaag ctaagatctc ttgatccgtc ttcaccaatt tctttgccgc
     8401 caaatctttt aagactttgt gttcaatcgg caaaccatga cagtcccaac cagggataaa
     8461 gaccgttggc gagcctttca tgtttttgta tttgatgatg atatctttta aatttttatt
     8521 gagagcatga cccaaatgaa tagagccatt tgcgtaaggg gggccgtcag gaaaaacaaa
     8581 gggctttttg ccagcgtttt ttgccaccat tttatggtag atatttttat catcccattg
     8641 cttaagaatt tccggctctt tagccggaag attgcccttc atagggaaat cagtgagcgg
     8701 taatcgcacg gttgatttat aatcaggaag tgtgctagac accaggccct cctcgaaaga
     8761 tttaagtctc agtatattca aaaaaagtta aatatttaag ccatttagtc ggaaactttg
     8821 cttttagaag cagacggtca aaggctcaag ggcaaagggt tttagccttc ccatagccca
     8881 aagtaatcaa caggcgcaaa gcttccagat cacaacttaa attgagctgt tcgcgaacct
     8941 cagaaatcat agctttgctt ttggcatcta gctggctcag catatccata gaaacctgat
     9001 tattttgaat atttacctgt ttgggttgct ggggttgcaa gggttgctgc acagattcca
     9061 ttggctggat cggcaggggc ggctggaaag taggagtcac atattgtgca tgatgaggca
     9121 tacgagaggt cgggctcttg ccttcgagtt cgcccatcat tcccgctaaa cttttaagct
     9181 cttttttgaa attttgaata cttggagcat ccagggactc atcaccactc cgctgcgcct
     9241 ttaaaaacaa gctgcataaa atatcaggag tcgtcgcgag ctctctgata gatgcattct
     9301 gatgtactag ccacatatag gctttcgtca gcgtatcttt ggtgtatgct tgtgggggca
     9361 agggattcat agtcatgagg gacaataaga tattgtccct gcgtcactca gtcaattctc
     9421 gagtccaggc tttatgggta tttttaggaa tatatttttc tacacattgc gaaatgagca
     9481 gatattcact tgataaaatc tgcccaataa aaaaagccga atcaaataga ctcggctttc
     9541 acacaaatcc tttaaatata ttgattttcc aaaaattatc cgaagactta ttttaagaaa
     9601 tctcccagca aacgtgcgcc cagaacgatt tctgttctag tttgggtatc tccgctaatc
     9661 ggttttgcac tgatcgtatt ataagctaaa tgatagcgga aattctcagc tgtgggtttg
     9721 tactccacaa cgactccata acccatgaat ttattggtcc cagttgttgt accaataccg
     9781 atttcttctt ctgaagagaa gagttcaagg cggggagtcc attgatctaa tttgtagcca
     9841 aatttggcaa tctctgaagt aagggtgtct ttgttgcccg aagctccatc cttataagtt
     9901 gtggtggtat attcaagact tccaacccat gatgaagtgt cgtatttaac cccggctgaa
     9961 atgaaattat gtttattgcc agtatctccg gttgcagatt gcggagaaac ttcatgatag
    10021 cttgctaaac cagtaagggc tttatcgaag aaagagccct tccaagttaa acccatcaac
    10081 ccacggtttt ggttgaagtt tccagcaggg tccgcagagt ccgcgatgtt atttaaaacc
    10141 attacgtaaa tatgttggtc caaaaagttg aaatcccctt tggcgcctgt catgtaaagg
    10201 atattggaag ctccggattg gttgataccg atatttgcac cgctcaaagt ttttacggct
    10261 gtgtgctgat agttcgggga aaccaaatag aggtcagggc cagcggtggc gccttcaaga
    10321 ccaccgattt cggtattcac cttccctaag gaaagagcaa ataggtcgct cattttatct
    10381 gtgacataag cgtactccac agagctattg agagaatctc gtttggcatt ggtaatcccg
    10441 gtatctactg cgggcttata aaaaccccag cgaagttggt agctaaaccg gtcattgagg
    10501 ttgcctttga aatcaagacg acctgttttt agatagaaac gcgtggagtc cggaagacca
    10561 gccgcggtgt tgtaagtata actttgccag tctccccgga aatcgtaatt aagagtgttg
    10621 gcatgggcac ttgcaaaagc cgtgagtcca cttaaaagag ccaaaattgc tttcatagat
    10681 aagccatcct tgttgttatg ttgaattttg aatatcaaca ctaacaacat tggttaggag
    10741 gggccaagga atatttatgt ttgattttgg tcaagactgg aaaagtccac ccttaaggtg
    10801 gactttgagg aaactagatt gagtaatctt ttgctacgat tccgtacttt tcaattttgc
    10861 gaagtaaagt tttcttggga atgtttgcat gcaaagcggt ttgattaata cgccctttaa
    10921 aagtttttag agctttaata ataaactctt tttcaaaagc ttctttttgc gcattgaaat
    10981 caagactttc attggtcgag ctaacgatgg ccacggcgcc ttccgcctct tcttcaagat
    11041 ctagagggtc gttatccaga gcgtcgaaat cctcttcaat ctcggcaact acgagaggaa
    11101 cactgggagc attgatgaga ttcacgccca ccgcagccag aaccgtctct ggaagtgaag
    11161 ccaaagtgat aatattattt gtttcaagaa caaaggcatg ctcaatcaca ttttcaagtt
    11221 ctcgaatatt acccggccat tggtatttct taagtgcgga caaagcgtcg gcggccactc
    11281 ctgaaatgcg tctgccttga gcagcattga attttttgat aaaaatattg atcaggtttt
    11341 caatgtcttc tttgcgatcc gctagggccg gcaagaaaat aggaacaaca tttaaacgat
    11401 agaacagatc ttcacggaaa gtccccagct tgatcatttc ttccaaagga cggttggttg
    11461 ccgcaacaat tctcacgttg gtggaaatct cgcgatttga acccacgggg gtgaacactt
    11521 tttcttgaag aacccgtaag atcttcactt gcatcatcac cggcatatcg cccacttcat
    11581 ctaagaacag agttccgccc tcagcgaact gaaacttacc aattttgcgt tgatccgcgc
    11641 ccgtgaaaga gcctttttca tgtccaaaaa gttcagactc aaaaagattt tcaggaatcg
    11701 cagagcagtt gatggccaca aacttttcat ctttgcgagc cgagttgtag tgtatcgcct
    11761 tagcaacaag ttctttaccg gaaccagaag ccccccggat caataccggt gtatctactt
    11821 tcgccagtcg atgaatgatg ttgaatactt tttgcatttg ttgggtgtta ccgatgatct
    11881 tgcgcccggc ttccaccaaa acgggtgcgg aagcggcgat attggagatc atggtgtgag
    11941 cagagacagc tttgtcaacc aaagtgagca gctcatcgcc cgaaaccggt ttagataaat
    12001 agttgtaagc accatctttg accgcttgaa ctgcgtgatc cagagtcgca tgggccgtga
    12061 tcatcaagac gatgatacct gggtcatgtt ctttgatcag cctcaaagct tcgagaccgt
    12121 tcagacgtgg catgtctaca tcgagaataa cgacatcaaa ttgtttttca gcggctttaa
    12181 ttttctcgat ggcattcaag ccatcaaaag cctcttcaac ttcaaaacgg ttcgttgaaa
    12241 tgagcgctgt tttaaccgcc aatcttaatc cttgatcatc atcgactacc aaaaccctaa
    12301 gcatgtgatc tcccctgttc caatggcagt tctaccgtaa aaatgctccc ttttccatag
    12361 gtactttcaa caaagatcct gccgtgatgt aattctgtaa aatatttagc cagatagagc
    12421 cctaaccctg accccttgat aggggaactc ttggcgtttt tgcttctaaa aaacttcata
    12481 aagatattat tcacttcatc ttgtgggatg ccgggtccac ggtcttctac gcggacaatg
    12541 accttgccgt ctttttcttc gctttggact cggacatgag aatcctcagg actgtatttg
    12601 atggcatttt ctatgagatt ggaaaaaact tgcttcatca actctggatc aacttgaata
    12661 gggaagaggg gctcgcattc aaattcaata gggatgcgtt tcactttagc cagaaattca
    12721 tgcttgcaaa taacatcttt gagaagggca tttacgtccc ggctttgcat gtggagttca
    12781 accccttggc tttctatttt tccataattt aaaatagcat tgataaattt taaaagatct
    12841 tcgtcggact gacgaatggt atctaaagct tctcgttgct gcgaactcaa tggttgagga
    12901 tccttcaaga taacatccgt catgccctga atccgcgcga taggggtttt taagtcatga
    12961 gacatcatcg aaatgaagtt ccctttcaat tcctcgacct gagaaagaag tttatttttt
    13021 tcgtagtatt cccaacttcg tcggttttcg atgatcagac gataaggaat aaagaaataa
    13081 tagcacagga agatcgctaa gaagggatga gccattttaa tccaatatcc tccaggccaa
    13141 aatcccacgg ttccgaggac caccaaagtg atcccagtga gagccaggat aatcagactc
    13201 cgaatcggct tcgtggtgaa aagaacgtga actgttaaaa tcgaagttaa aatagttacc
    13261 aaaatattaa gccaaggaga ggcttgaatc ggagctttgt tgcgaatcag agtatcgaac
    13321 atgttggcgt tcagctcaac cagggtcatg gccttggcat cactggagaa aggagttttg
    13381 gcataatcac ggatggctcc gccatgatca ttcccaataa gaacgatttt atctttaaat
    13441 cgaccgggcg caaatcgacc atccatgacg tcttcaaaag aagttcgtgg ataggtattc
    13501 gctttaatat agtttatata aacctgttcg gagtcgaaga atttaaaaag accgcgaata
    13561 ttatttccgt ctttgatgac cgggttgacc agccccgcga taaaggggtg gaccatttga
    13621 cgatcctggt aagtaataat catccgacgc gtgaccttat cctgagctaa gacgtcccca
    13681 tcgttagtgc ggggaccggc aaagagctca atcgaactta aaggttcgga gagcttgagc
    13741 tttccttctt cccccttcat ctcgagatct tggctaatca tgtaaagatt cttgattttc
    13801 tgtgcttcct tggcgaaagt ttttttttct tctaaagttc cttgaagctc gtcgaggttt
    13861 tgggtgtata ggacggcaag gggtccttcc ttgcccagat tttttaacat ttccaattgt
    13921 tctttagctc ttggtaagcc cttaagatct tccacagttc tggaagtcgt catgattatt
    13981 tctatatgac cggagggcga gggttcgggt agcagacgta ttcgcaaatc gtagagcccg
    14041 gcttcccaag aatctaattt gagctgactt ataaatattc ccatcgcaca agctaaaacc
    14101 aggcgtaaga cagagtacaa gaccccgcct ttaaaaaagc ctgttgtttt agtgagcgac
    14161 ggaggaagtg ccatattagt tttccgtaat caaatcgcca cccacaggag ggcaggggtt
    14221 gcttccattg ccgtgaccga aattagctgt ttgtgtggtt ttcggcttga gtttttttac
    14281 atgcttttta tctacttttt ccattatttt ttcctttatg agagtgagga acttcatctt
    14341 ttctcctttt tattatcccc caagggagaa tttcttaatt attaagcagt gcttcgacat
    14401 ctttcataat aaatgagcgt taaagccacc ataaatcttg tgcgctcaca gaattgtgag
    14461 tttttaatgt ttttatcgcc agaggcatgt ttatgagcga acatgcagcc tccaccacat
    14521 aaaaatcggg cccagcaatt ttcgcaatga ttaacttcaa gaagcggctt ttgataggct
    14581 gccaaagctt tttcattgag ttcctcgccc tggcccacct gctccttggg atcgtttaca
    14641 tcccagggac aagtataaat tcgattgcga gcatcgagca tcaaaaatga ttttccggct
    14701 ccacagtgat tctctgtgcg ttgttggtga tccaaggctt ggaaatactg ttcaaatccg
    14761 cgaatttttc ttaaggcgcc ttctccgcct ttagcgaaag cggcggcggc tagtttggtc
    14821 atttgctcca tgtactcaac agtggctctt ggattgttct cagaaacgga gaatgaaaaa
    14881 tcgtaactat caacagcgaa atgagaatag aattcgtaag ctttttcaag ctccatatta
    14941 tggctatcaa agacaccgtg gatcatgatg tggctgaatt cagatttgcg agccaacagt
    15001 ttttcaagac cggcaacgat cattgcggta gaagaagtgc cattcttagt cggtcgaacc
    15061 acatcattga tttcgggcgg tccatcaaga ctgattgtca ccgttgcttt catagccgct
    15121 aaaatatcga cgtttttgtc cgttagcaat gtgccattgg tgataacggt gtaattcact
    15181 tttcttccgg gagcgacttc agcgacatag gttgcaatca aggccaaagc atccgcataa
    15241 aggagcggtt ccccgccgag aaagctgata ttaaagctca cctttggcgg caaacgatca
    15301 agaaagaatt tcagttgcgg caaagtcttc tccagagaga tcttagcgat cgcatcgccg
    15361 aaagtgccgt cgcctcccgc cgcacagtaa ttgcaatgca ggttgcagat ttgcgtgacg
    15421 ttcaaggtca gaaaacgaat cgtcgaatca attttgcttg ttttcacttc ggaattgttt
    15481 tcaatctccc aagactgaag ctcttcaagc gcttcacatt ctttttggac gcttcccggc
    15541 tccatattcc gccaagtatc ctcagagatt tgcgcgactt cgagattgcg cgcatggaag
    15601 gccagaaggg ttccctcttc tgattttagc gccaaaatgt ctttatacct tcgcaattcc
    15661 attgagcttt ctttggggac tatttgccac caatgattcc acaaaacgat tccaaaggtc
    15721 caattgtgca attagggggc cagaattagt tttattgcct taaatgaaaa gaaagtaaaa
    15781 acagaggttt aagctgtctc agtgaaaata aattaaaacc tgtattggag ccacaatgta
    15841 actattactg aattgtctag agttaaatga tctcaaagct tctttcttct tttttacccg
    15901 atactttctc ccatactttt accggacttc tgattgtacg caatcagaag taaagtcgcg
    15961 tcccaatttt ctcttcgtat tccaaaatcc ttcgcgactt atgcaaacca gacatttctt
    16021 ttcctctttc ccttaatcca ttccttgatc ctcttcatcg aaagagcctc tgaagcttta
    16081 atcccccctc aagaaaattc ttctccaaga aaaaataagt tgacggcact ttctcctctc
    16141 aagaattcaa tacaaataac tttttttcct ctcaccctcc ctttgccgat tttgtattat
    16201 ttgtagaatc cgagagttta ggagtttagg atacatttaa ttcaggacta ggcactcctg
    16261 atgaaattct ttaagggcat cttcttcatg gctaaagctc accacaatca tttgcgggaa
    16321 actggcaata agagtttcat agaggaattt ttcggttttc ttctcaagcg ccgaagtggc
    16381 ttcgtctaag aaaacgatct cgggtttttg caagaagata cgagcgatgg cgattcgttg
    16441 ctgctctccc gaagacaagg ataacgacca cgaagtttct ttatcgagat caggaattaa
    16501 ataagcgagc ttggtgagag tgagaacttt ttgaaaatct tgatcattga aattttcccc
    16561 agccataggg taagccaagg cggatcgtag attgccgatt ggaagataag ctttttgcgt
    16621 gagcaccagt cgatggcatt cttttggaat ccgcacttga cccgcaaggt agggccaaat
    16681 gccgttcatg cttcggagta aagtggtttt tcctttgccg gagggggcgg taattaaaac
    16741 tctttttcca ggagtgagtc tcaaactcag cgcattgaag agactctgat gttgcggcgt
    16801 catcacgttt aagttctcta tttcataagc gccgacggtg gtttgcacgc ttcgcgaaac
    16861 cgtggcgtgg cgaagttcgt gattttcttc caaggaatgc aagaatccac tcagacgaat
    16921 cataaccgaa tgcagacggg cgagctttgt gtacatatca acgaaaacag agacggatcc
    16981 ttgaacttgg ccaaaggcgc tgctgatctg aaacagttgg cccaaagtta tctcgcgggt
    17041 gaaaactttg ggggaggcca ctacaaaagg aaaaatataa gcgagctggc cataaaaatt
    17101 tgtggtcatg atcagtttct tctgacagga gatcagttct ttgaaattac ctacaacttg
    17161 aaagaattta gcggaaagtt tttgtttctc cgcgcgctcc cccttgagca gagcgatgct
    17221 ttcactgttc tcacggaggc ggatgagaag gtagcggaaa tctgcttcca gtttttgctg
    17281 catgaaattg attttcgaaa gcggactccc gattttatgg acgatccagg tacccacaat
    17341 ggcatacaaa agacagaccc aaactaaata acgatgaatt tcgatatcat aaccattgat
    17401 atcgctgaac tttagggagc ccgagagtcc ccatagaatg ccgataaaag aaataaaggt
    17461 tataaattcg cgcagaattt tttctgaaat ctcaaggctc aggtcggtca gctcgtggat
    17521 atcttcggag agtcgttgat cagggttgtc ttcagaattt ttcttaattt gccaataata
    17581 gaaattttga tttgaaagcc aatgggtgac atttttttca gtgagccatt tcctccagct
    17641 gactgccaga ctttggagga cataatactt ataggccatg atcagcgagt aagagagggc
    17701 gataatgaaa aactgcctta gttggctgag gaattcttcc ttgttgagat tttggagggc
    17761 atcgtagaaa gccttgttcc agcgattgag taaaaccaag ctgtaaacca taccgagaat
    17821 caatactaac ataaggagca gattcccacg cgccttccaa cgatcttcag aacgagtcca
    17881 gtaaggaaat acaagttctt gcaaaacttt tttgaccgcc gagcttccca tgagaacctt
    17941 ctagtagact tttagtgcta ataatcattt aattttgcag tactagctag aggagacgca
    18001 agagaatgtc atcaaaatac gttgtcaggg gattgctcag tctttttctg ggtatcggga
    18061 ctcaggtgtt gttaggtcga gaggcaagag cggctttcgc gaactataat tctattttaa
    18121 ttggtgacca ggcggcggga atgggcggag cttatactgc catggccaat gacgcctctg
    18181 cgatggccta ttataatccc gcgggcctgg ctttcttgga agggcacact ttttccgcag
    18241 cggtgggcat ctacaaaaaa tttgatacga ctttcggtaa agaagaagac tttaccaaag
    18301 ctcccttacg tatgaatcaa ggattctttc gtgccattcc ttcttccacg ggaagtgtga
    18361 ttaaaaaagg ggattacagc ctggcctttt ccatcgtggt tccagatttc gataattaca
    18421 aaggggattt aagagcaggt tcggataata ccacgacttt gtcttatgtt gatgaatcgc
    18481 tttgggtggg aggcgccgtc gctaagaaaa tttctgagac tgagtcttta ggactcacga
    18541 tgtattatac cgcgcgcagc tattcacgct ctttaaatga tcgaagtttt ccaacggtga
    18601 ccgaagctat tttattcaac tcggaaaaaa ctttggtgga gaatgcggtg gttgcggttc
    18661 tgggttatca tttgaatctc tctgagaatt ggtcttttgg cgcgagtctt cgcactccga
    18721 acctctccgt gaagggaatg ggttcttatt tttcttcgca agtgacaacg aatccttatt
    18781 ctgtaacttc ggtgaatttg ccggatgagc ggaccaacgt gagaattcca ggcaagctag
    18841 ccattggaat tgcctatcgc gatgttgatg atttcactat ttccgcagac gcaacccttt
    18901 atcaggggct tgaatatgat gactttaaca atcaaagctt tacgactcac tttattcatc
    18961 ggcaaacttg gaatttagcg gcaggggcgg aaaagaaagt catgccctgg ctaaaggtac
    19021 gtgccggagt tttcacgaat ttttcttccc atccaacgcc agacccctca ctcaatgtga
    19081 gccagccaga taaaattgat caaatgggct ttagtgccaa tgccgtgttt gtttctggag
    19141 ataaaattga ttataccttc gggggatatt atacgggcgg tagaggtaaa tccatccagc
    19201 aaatcaatca aaatgctgaa gaagtgatcg tgactcaaca tgtatttacc atgttggtgg
    19261 gaactgcttt ttcgttttaa ggctaggtat ggacaatacc tcagtagagt cttaaacccc
    19321 gtcattaaaa tttatttgca catgtaagga tcgctcgcca tccttacatt atatgtcatt
    19381 aactgtgaag ttctggggag tccgaggttc gcttccatcc tccccctcgc cagccgaatg
    19441 gaccaatcat attgaaggac tgcttcgggg tttcttcaat gcagggttgc gtgatgccac
    19501 tcaagtttct aaatatattg attcattagg ggcctctcac ctaggaggtt acggagcggc
    19561 gacaacttct gtggaagtta aaagcggaaa aaatcgtatt atcatagatg gaggaagtgg
    19621 cattcgctct ttgagtgaac agatcatgag cgggaaagcc ggggccagta aaggtccttt
    19681 ccacatcttt atgacccatt tccattggga ccacatcata ggcttgccat ttttcactcc
    19741 tcattttgta ccaggagtac aaattcatta ctatgcggtc cagccagaaa tggaaaaatt
    19801 aatccgtggt atttttacga aaccgtactt tccagtgcct tttgaatcgc ttgcttcgaa
    19861 aatttcattt catgttttgg aaccgcgaaa aaccgttgaa atcaatgata tgaagatcac
    19921 tccctataag ctagaccatc ctgatccttg ttggggattc cgcgttgcat gtggtgataa
    19981 ggcttacgcg cattgtgtgg atacggaagg aactcgggtt tctcgagaag agttggcgga
    20041 agatctgccc ctttatcaag ggattgattt aatgtactat gatgcccagt atacttttcc
    20101 agaactcgcg gaaaaagcga actggggaca cagtgctgcg caagtgggac tcgatcttgc
    20161 ttttcgtgaa ggtatcaagc acgttctttt cgctcatcat gatcccggtg caacgattca
    20221 acaggtgcag gaattaaagc gccaaactct tgagtattat gactggcgtt taaccacggc
    20281 gaaagaaaat gcggaggcgc tcaatgcggt tcaatgggac ttcgcttatg aaggccttac
    20341 tgtaaaaatc tgagtctact taaaatgtga ttagaatctg aagagccctg gttcggtgcc
    20401 ttttctaaag atcagttcca cgtttgttgg gtcttttgtc ggaatctcca tttcttggag
    20461 tttgttttca tcgaggttga ctttcacagt gccttccggc caagcaaaat cttcgacagg
    20521 atcttctatg gtggcgtgct tcatgaaatc tgtccaaatc ggtacggcac cactcgctcc
    20581 agtgagtttg ttcatcgtgc ttgtatcata cccaatccat acaatggtgg tgcgataagg
    20641 agtaaagccc gagaaccaca catctcggct gtctgaagtg gttcccgttt ttcccgcggc
    20701 cggacgagtg aagccactgg cgctcacata tttggcggtt ccagagacaa tggtttgctt
    20761 catcattccg acaagacttg ccacagctac aggatcttgc acctgttctg cccgtggcgt
    20821 aaaatgataa acttcttc
//
LOCUS       DATBTE010000012         2946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1231301, whole genome shotgun sequence.
ACCESSION   DATBTE010000012 DATBTE010000000
VERSION     DATBTE010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231301"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VIG33_00395"
     CDS             <1..806
                     /locus_tag="VIG33_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269318.1"
                     /translation="QYHHKPRKDEAILKNIAGNDVSIFLYRFELRGNGIDFVLNEAIA
                     EDMYPDIDEKMKPLVHACCETLLRYRHLSVSNTIMDGNFLVTGEFEVMLSKGLGQHFA
                     HDEKTRLFQDAKYIADLLGEVMDRGTQELEKGIPRNQPPIEHTPNPRKIKKGLEKLGK
                     TKHQQAKLQWLAEGVPLRPGLRQLRPDDLPPDVTASSGYDHRGLCYVFDHKKFGELGR
                     IVLIQVREQEMLMQADLYIGQEKQESTIAKKKKAIFEEVVTTINACFNL"
     gene            complement(1098..2717)
                     /locus_tag="VIG33_00400"
     CDS             complement(1098..2717)
                     /locus_tag="VIG33_00400"
                     /inference="COORDINATES: protein motif:HMM:NF021594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
                     /protein_id="HEY8269319.1"
                     /translation="MLKEIQCPLFNHARITFHQGLNIILGDDDAKNSIGKSTALMVID
                     FAQGGDSFLKDEAGAIRALGHHHYNFSFVFAGQPYFFSRSTDASDVVYVCDNDYARLG
                     ELPVEDYRRKLKELYGLASLESSFRSIVSPFARIWRKGGLEPDQPFISAPKEPSGTAV
                     SRLIDLFDRSADITAEKKVIDEQKKRKKLIAGSMNANIIPDINKTKYKANTKTIAENT
                     VQIEQLKQGFSGALNAYEALFDESLRRMQQRKNELTNLRSELQSKVKRLQREISGITP
                     RLAANIALVAEYFPTVDVERLEQVEAFHQKIGRIVKKELKDELAAAQLKESALTSEIM
                     SLEQQIQSALKSKGMPDDLFKRIFDLKEITDKAAEENKYFEQKAQLEEAIKISGHRLD
                     EIYAKIFLEIEAKINLKLKAFNKVVYGPARNSSELRIKSSNSYSFTSPEDSGTGKSYA
                     GLIGFDMAMLSLTRLPFVIHDSVVYKNIEVAAVQRILRILAVIKSKQIFLSFDEAKKF
                     GLQTEQLIKKFTVLKLSHNDLLYNKDWRDKK"
     gene            complement(2710..>2946)
                     /locus_tag="VIG33_00405"
     CDS             complement(2710..>2946)
                     /locus_tag="VIG33_00405"
                     /inference="COORDINATES: protein motif:HMM:NF042476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 7"
                     /protein_id="HEY8269320.1"
                     /translation="IARSLMLVPSKFTRLEESTIFKMKCILANKKESESVLDVYLRTK
                     DFFYDASEFLHALDVLYVLGLVNIKDDSGLIEYA"
BASE COUNT          841 a          680 c          610 g          815 t
ORIGIN      
        1 cccaatacca tcacaagccg cgtaaagatg aggcgatttt gaaaaatata gccgggaatg
       61 atgtatctat ttttttatac cgctttgaat tacgcggtaa tggcatcgac tttgtgttga
      121 atgaggcaat tgccgaggat atgtacccag atatcgatga gaaaatgaaa ccgctcgtcc
      181 acgcctgttg cgaaacactt ttgcggtaca ggcatctcag tgtgagtaac accattatgg
      241 atggcaattt tcttgtcacg ggagaatttg aggtcatgct gagtaagggg ctaggacaac
      301 actttgcgca tgatgaaaag acgaggttgt tccaggatgc caaatatatt gccgatcttc
      361 tgggggaggt tatggataga ggaactcaag aactggaaaa aggaatacca cggaaccaac
      421 ccccaataga gcatacgcca aatcccagga agattaaaaa agggcttgag aaactgggaa
      481 aaacaaaaca ccagcaggca aaattgcaat ggcttgcaga gggcgtgcca ctacggccag
      541 ggttaaggca gttgcgtcct gatgatttac cgcctgatgt caccgcatca agtggatatg
      601 accatagagg gctttgttat gtcttcgatc ataagaaatt tggggaactg gggaggattg
      661 tattgatcca ggttcgggaa caagaaatgt taatgcaggc cgatctatac ataggacagg
      721 aaaagcagga atccactatc gcaaaaaaga aaaaggcaat atttgaggaa gtggttacaa
      781 ccatcaacgc ctgctttaat ttgtaaccat caaaaatagg acaaatcact tcccacaacc
      841 tttcaaacct cgccgcattg gtggcggttt tgatcggaat acgcagtttc cgccactctc
      901 aattaatttg gttcctagcc gatgttatcg ggctgttatc aattaatctg ggtccattct
      961 cacttaatcc gattcctgat gcccggatat tctcagttaa tatggtcccg aaaaccggat
     1021 gtaatcggtt cctgcgattt atgttatcgg gccgctacac ttagctttgg gctgtccagt
     1081 tgactgaaag gttagctcta cttcttatcg cgccagtctt tattgtaaag aagatcgttg
     1141 tgactcaact tcaagacagt gaatttctta attagctgtt ccgtttgaag gccaaatttt
     1201 tttgcctcat caaaagacaa gaaaatttgc tttgatttga taacagccaa aattctgagg
     1261 attcgctgta cagcggcaac ttcaatgttc ttgtagacaa cagaatcgtg tatgacaaac
     1321 ggcagcctgg tcagagagag catggccata tcaaatccga tcaagccagc atatgacttc
     1381 ccagtccccg aatcctcggg ggaggtgaaa ctataggaat tagaactttt tattcgcaat
     1441 tccgagctat ttctggcggg gccgtaaaca accttgttga atgccttcaa cttcaaattg
     1501 attttcgcct caatctcaag gaagattttt gcatagattt catccaatcg atgaccagat
     1561 atctttatcg cctcttcaag ctgcgccttt tgctcaaagt atttattttc ttcagcagct
     1621 ttatccgtga tttccttcaa gtcaaatatg cgtttaaaca agtcatccgg catacccttt
     1681 gacttcaatg cagattgaat ttgctgttcc aacgacataa tctcgcttgt aagagcagat
     1741 tctttcaatt gagcagcagc aagctcatct ttgagttcct tcttaacgat acgtccaatc
     1801 ttctgatgaa atgcctctac ttgctcaagt ctctccacgt ccacagtggg aaagtattca
     1861 gccacaagcg caatatttgc ggcaagtcgc ggcgttatac cggaaatttc ccgctgcaat
     1921 ctttttactt tgctctgaag ctcacttctc aaatttgtaa gctcgttctt acgctgctgc
     1981 atacgccgca agctttcgtc aaacagagcc tcataggcat tgagtgcccc actaaacccc
     2041 tgcttgagtt gttcaatctg cacagtattc tcagcaatag tcttagtgtt tgctttgtac
     2101 ttagtcttgt ttatatcggg gataatattc gcattcattg aaccagcgat gagcttcttg
     2161 cgctttttct gctcatcaat caccttcttc tcagcagtaa tatccgcact gcgatcgaag
     2221 agatcaatta aacggctaac tgctgttcca gaaggctctt taggtgcgga gataaatggc
     2281 tgatcaggct ccagccctcc tttcctccaa atgcgagcaa agggactcac aatagagcga
     2341 aacgaacttt ctagacttgc tagcccataa agctccttta acttcctgcg ataatcctcc
     2401 acaggcaact cgcccagcct tgcataatca ttatcacaga cgtacaccac atccgaagca
     2461 tcggttgaac gagagaagaa ataaggctgc cccgcaaata caaaggaaaa attatagtga
     2521 tggtggccaa gtgctctaat ggcccccgcc tcatccttaa ggaagctgtc gcctccttgt
     2581 gcgaagtcta taaccatcaa tgccgtagac ttgccaatag aattcttggc atcatcatcg
     2641 cccaatatga tgttgagtcc ttggtgaaaa gtaattcttg catgattgaa caaagggcac
     2701 tggatctctt taagcatact caatcagccc cgaatcatct ttaatattca ccaaacccaa
     2761 gacgtacaag acatcaagcg catggagaaa ctcgctagca tcgtagaaga aatctttagt
     2821 tctgagatat acatcaagaa cactttcgct ttctttttta ttagccagaa tacatttcat
     2881 cttgaagata gtggactctt caagcctcgt aaacttactc ggcaccaaca tcaaagacct
     2941 cgcaat
//
LOCUS       DATBTE010000013         9896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_343438, whole genome shotgun sequence.
ACCESSION   DATBTE010000013 DATBTE010000000
VERSION     DATBTE010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9896
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343438"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..776)
                     /locus_tag="VIG33_00410"
     CDS             complement(90..776)
                     /locus_tag="VIG33_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEY8269321.1"
                     /translation="MSLEVVREECYDLLKKLHRTRENLKILAVSKLQPAEKIRRLHAV
                     GHKLFAENYVQEALLKQETLKDLDIEWHFIGSLQKNKVKLVVGKFSLIHSVDSFELAK
                     GISAHAEKIKTIQKVLIQVNLAHEISKGGFDREAFLLNWPEISAFPGLEITGLMTMPP
                     LTDDPEDVRSYFRELRELQIELQKRGTGRHHLHELSMGTSHDYPVAIEEGATIVRLGT
                     ILFGERPRKN"
     gene            complement(773..1375)
                     /locus_tag="VIG33_00415"
     CDS             complement(773..1375)
                     /locus_tag="VIG33_00415"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY8269322.1"
                     /translation="MYQLILASESPRRRQLLQTAGFVFDVHPVKVSEIPDKNLNIDEQ
                     ILDIARRKAKVAFSQLKASFHKDFLVLTADTMVVYHTQTLGKPRNPDEAYEFLKLLSG
                     KTHEVKTALCLVESLAGQTASHLETSQVIFRHLSNDEIWTYIQSGEPLDKAGSYAIQG
                     VGGKFVEQFIGEYDNIVGLPVNAFEKLLAEKKWSVERVKR"
     gene            1404..2315
                     /locus_tag="VIG33_00420"
     CDS             1404..2315
                     /locus_tag="VIG33_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY8269323.1"
                     /translation="MGSVDLLLIAGFILGGLSIYLFVSALFSSNQDSQQLSWANNSEP
                     AKSKSPVINFSRPLVHQFTLQHAARIKNVGYRKRVRKHILTSGLSSELNEDEFIGLQI
                     LWGVMFPVFLYVMNFALSLNLPAPLLICMGMGGFVFPQMHASAEKKRRELSVRGDLPF
                     FIDLLALSVEAGLDFFAAISKIVDKAHGQDSVLADEFGTVLKDVKIGASKTQALKDMA
                     ERLDIPEITSFVAVLIDAEASGASISKVLKDQSVQMRLERFVRAEKAGARASQAILIP
                     LMLFILPAVFIMVFGPVAVSFIYGGGK"
     gene            2319..2666
                     /locus_tag="VIG33_00425"
     CDS             2319..2666
                     /locus_tag="VIG33_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY8269324.1"
                     /translation="MAVLKSETKNMQLISDLEVAATEWTRGKGLLGRKLLGPEQALWI
                     RRCNSIHTFFMKFAIDCVFLDKQLRVKAIYPHVKPFRLVWPVWGATSVIEFPAGRAKQ
                     LNLEIGEQLNVGS"
     gene            2653..4284
                     /locus_tag="VIG33_00430"
     CDS             2653..4284
                     /locus_tag="VIG33_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY8269325.1"
                     /translation="MWAVRVLTGPLAGQIYDLRMGRNLIGRAPHCDVKINSHGVSKEH
                     AEIHVYKDKIMVADLKSSNGTYVNGVRAQNGLIRLGDKISVHDVIFDIIPAPDIRPTS
                     PAAVSSPLKGPPIHGGNAAPNYYSQQAPQMMGDLNPGVPAPESVDKILPPQGVMQRLL
                     FQVNDYLERVALPGVYKLAQLMEFKYILGGFVILFIFAVTLLSLIPMVQITRSSVLVE
                     SKRRAQSIARNLAISNQQMLLQGNISSLSTHLAETEDGVKEVMIIQASDGMIVAPASR
                     AGVTPKIPFVHAARREGKTQVEQIDSQTIGASFPIGQYDPNTGEPSVKAHAIVLYDIG
                     SLAFDDGHVLSLFFQTLILACFVGLGLFFFMYKLIEFPIMSLNNQLDTALREKKDSTD
                     VNFQFPVLQNLVGNINSLLSRYTHGGDHGGEPRNMVSHDVEAENLVQLMGYPTIAISG
                     ENRIISVNGNFEQIAHSTRAQLNHQTLSAIPDAALQQNLQHLIQKSREVPGTIHSDQL
                     EFSGHNCRIHCQAMGVESSTIAYFVICITPLEGSE"
     gene            4284..5354
                     /locus_tag="VIG33_00435"
     CDS             4284..5354
                     /locus_tag="VIG33_00435"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY8269326.1"
                     /translation="MAAPQLKNTLKYKIMIQRGPLAGQNYSFDKEVVQIGRSPDNDLI
                     LSNDLRASRNHAEIRWDGVDLRITNISSKNFLAVNGSQVESAKLESGTIVVVGETEFY
                     INLEGVSEKEIAEPKTKVNFVEAVEKAVPGFTQHIPVLQQTHSSPVDFIPPVNPRGMP
                     ASAHPSQNLARSSPRTIKKVSNSTLRFYVIVAVIGIAFAWLMSSNVAKKKELSFRSAE
                     QIEKDIESSREEMKEFETRKEKLDNVQFRKAQENYIRGFRDYKQGNFGRAKESFQVVL
                     NLDPENELATRYYQLAKLKFDELAKFHMLQGNRYREKKNWRMCKSSYFNVMTMVSNNQ
                     DPLFKEARQYYDQCSLAQEGRY"
     gene            5354..7306
                     /locus_tag="VIG33_00440"
     CDS             5354..7306
                     /locus_tag="VIG33_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY8269327.1"
                     /translation="MARLRVRLHGKVIQEVQLSPERSYLAGRREDCDIVLSNGKAISR
                     EHFKIHFSEDQWQLEVLSRFGDVVVNGEKIQQAILEGQTIIYLRPYEFEYSEISSDLV
                     SVPPSVNTPEPLFRPAFEEADEVEKTFIGSVDIVPYIKVISEVGEVKELFKLEGGNTW
                     VAGRDPSCDIIIRDQRVSRRQFEVRRQGNLFQILDLGSVNGTLVNGNPVSSTDPINIK
                     SGDAIGVLDNHLYFELHDPDFKARMELVKTNVLNPLVALNHEGEPSLLVQDPQHMESH
                     LGYSYPAHGTPPQPNTQKKFDFEKHRLKLIAGAVLLIAVAYIFSDQSGTTPKTTQPPA
                     EGVLNKLSPEQKILIKQTYMLAKNLYMQGKYELAKAEVSKIYELVPEYEDTKDIERLA
                     NEALAIQEQKRRQEELEKSKAETEEKIQKVADECKAKINADYTSSELEECLSSVLQFN
                     PDHPLFAQLRHQVEERMNNKKIKEAQKAEYQSQVQKLHGLFNKAEASSKENQYLRALK
                     EYQTVSTSGLPDPHDLKVQARKQMAAIQQVLKQKTDQFDQEADAAYKDQKLKDAILVL
                     RKSADLNPNNNDTKDKISRYILELKKQMMILYQEGVLEESYGNVDGSENRPGAKDKWK
                     KIMDLDIPDGEYYGKAKIKLKKYGLI"
     gene            7306..7986
                     /locus_tag="VIG33_00445"
     CDS             7306..7986
                     /locus_tag="VIG33_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269328.1"
                     /translation="MRIEERSYSTKIVRPRPAIYIEPDGSFIVISTSWGDPEHGLKVN
                     DDIAKYIQAAKADVEVTSPFEFLTSLSVEANYLRIATLISNESLYRGSNKTEYQAGVE
                     TLLLLRKGSLMAYAQVGSPHLLIQKKNQLVTPISVGFEAVLEMSGEDQDLLSPLPQSL
                     LGIDGNVNVRTGDFRIDESDRLLLYGGSVWPKSLWSPDCDFQQLTQKLVQASPESPFW
                     LGVVSLED"
     gene            complement(8011..>9896)
                     /locus_tag="VIG33_00450"
     CDS             complement(8011..>9896)
                     /locus_tag="VIG33_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY8269329.1"
                     /translation="IKSLSLNPMRTVKASSIIVILLVLTTGLVQLSCQSSTTPSILIV
                     AVDDLSVLDILCNEEMTNATKSGFEILCKESVRFTHAFNPSVLTVPTLTSILTGVYPF
                     QHRVRHNGQPGLDSQFVTVAETALEKNYRTAFFSGGPPVWRKSGLNQGFELFEDNIIP
                     SKQSLYRPFTTSTKSLLQWIRQDVGHSSPFFAMVYVPDLIFTNTVTTNEFGEARNLSH
                     ESQLDELNSNLYSFFRELKTIQRWDNTTLIVVGLNGYASDDRPGELNPSNLHSENTQV
                     ALFIKPPRKSHDEALQWKIDRNVSLVDLGESLYELLGAPKNPNKPAEIFPVYSLSQLV
                     QNKPMAGAENRFILLESDWSQWRGFSNRRSAVLSNHILFIYDQYPQIFNTLTDREEIH
                     PLRSNELPQEERQGFIAALDAVEAEPWIGYDQEFINKYTPAFSRWMRADQKDTLFKDV
                     KIKINGKNPDPDILKWAALLSFELKDWNSLKAISQKAEQPFWQMVAEKNGADETNVHF
                     NLFQDFCLRLLEIPKLDSVDLKKCDNALFLDFIDWQRADARNFSKSTQKRRFERSYLN
                     FLVDQNIKQGNLATLLIWDTPDKFQPSPELVDLLLSLPEFQKQRNNLMKSLQNSSNWQ
                     DDD"
BASE COUNT         2749 a         2073 c         2307 g         2767 t
ORIGIN      
        1 ttatggcttg cgccatattg ccagcgccca gaaaaccaat tttttggcgt tttaaaagtt
       61 gattcatggt acgtccctta atgtgtcctt tagttctttc gtggtctttc tccgaagagt
      121 atcgttccaa gtctaacaat ggtagctcct tcctctattg ccacagggta gtcgtggctg
      181 gtgcccatgg ataattcatg caaatgatgt cggcctgttc cacgtttttg taattcaatt
      241 tggagttccc tcagttctcg aaagtaggat cttacatcct cagggtcatc tgttagaggg
      301 ggcatggtca ttaatccggt tatctcaagt cctggaaatg ctgagatttc tggccaattc
      361 agcaagaaag cttcacgatc aaacccccct tttgagattt catgggcgag attgacctga
      421 ataaggactt tttggatcgt ttttattttc tcagcatgtg ctgaaatccc tttggccagt
      481 tcgaaggaat ccactgaatg gattaaagag aacttgccca cgaccaattt cactttattt
      541 ttttgcaagc tgccaatgaa gtgccattcg atatccaggt ctttcagtgt ctcttgcttg
      601 agcaaggctt cttgcacata gttttccgca aagagcttgt gtccgaccgc atgaagacgc
      661 cttatttttt ctgctggctg cagtttggac acagctagga tctttaagtt ctctcgagtg
      721 cggtgcaatt tttttagcag atcataacac tcttcacgaa caacttctaa gctcatcttt
      781 tcactctttc aacggaccat ttcttttcgg ccaacagctt ttcaaaagcg ttgacgggga
      841 gtccaacaat attgtcatac tcaccgataa attgttctac aaattttcca ccgactcctt
      901 gaattgcata gctgccggct ttatccagag gttctccgga ctggatatag gtccagattt
      961 catcattact taagtgacga aaaatcactt gggaagtctc aagatgagac gccgtttgac
     1021 cagcgagact ttcgactaaa cagagcgccg tcttcacttc atgggttttt cccgataaaa
     1081 gttttaaaaa ctcataggct tcatcaggat tacgaggttt tccaagggtt tgagtgtgat
     1141 aaacaaccat tgtgtctgcg gttagcacca agaaatcttt atggaaagag gctttcaatt
     1201 gcgaaaaggc gaccttagcc ttacgtctag ctatgtccaa aatctgttca tctatattca
     1261 ggtttttatc cggtatttcc gataccttaa ctgggtgcac gtcaaagaca aaaccagcgg
     1321 tttggaggag ttgtcggcga cgtggagact cggatgccaa aattaactgg tacataccat
     1381 aacttgaaca taaagagagt taaatgggaa gcgtagattt attactcata gcaggtttca
     1441 tcttaggagg actcagtatt tacctttttg tctctgcatt gttttcaagc aatcaagatt
     1501 cgcaacaact ttcttgggcc aataacagcg aacccgcaaa gtcaaaatca ccggtgatta
     1561 acttctctcg tcccttggtt catcaattca ctttgcaaca tgctgcgcgt attaaaaatg
     1621 taggatatcg taagcgagtc cgtaagcaca tcttaacctc gggactgtcc tctgaactga
     1681 atgaagacga attcattgga ttgcaaattc tttggggagt catgtttccg gtttttctgt
     1741 atgtgatgaa ttttgcgttg agtttgaatt tgccagcgcc gctcttgatc tgcatgggaa
     1801 tggggggatt tgttttccct cagatgcacg caagtgcaga aaaaaaacga cgagagcttt
     1861 cggttcgggg agatttgccg ttttttattg atcttctggc tttgtcagtt gaagcgggtt
     1921 tggatttttt cgcagcgatc agcaagattg ttgataaagc ccacggccag gacagtgtgt
     1981 tggcggatga atttgggact gtgttgaagg atgtcaaaat cggagcatct aaaactcagg
     2041 ctttaaagga tatggcagaa cgcctggata tcccggagat tacaagtttt gtcgcggttt
     2101 tgatcgatgc cgaggcgagc ggagccagta tttcaaaagt tttaaaggac caatcagtgc
     2161 aaatgcgctt ggaaagattt gtcagagcgg agaaggcggg agccagggct tcgcaagcca
     2221 ttctgattcc tttgatgtta tttattttgc cggcagtctt tattatggtg ttcggtcctg
     2281 tagcagtgag ctttatttac ggcggtggaa aatagaggat ggcagtgtta aaaagtgaga
     2341 cgaagaacat gcaattgatc tctgatttag aagttgccgc gaccgaatgg actcgcggca
     2401 agggtctatt gggaagaaaa ttgttagggc ctgaacaagc cctttggatc cgacgttgca
     2461 attcgattca cacttttttt atgaaatttg ctattgattg tgttttttta gacaagcaat
     2521 tgcgagtgaa ggccatttat ccgcatgtga aaccatttcg tttggtctgg cccgtgtggg
     2581 gagcgacctc agtgatcgag tttccggcgg gccgagcaaa acaattgaat ttagaaatcg
     2641 gagagcaact caatgtgggc agttagggtc ttaaccggcc ctcttgcggg ccaaatttat
     2701 gatctgagaa tgggccgaaa ccttatcggc cgggccccgc attgtgatgt gaaaattaac
     2761 agccatggcg tttcgaaaga acacgctgaa attcacgtct ataaagacaa aatcatggtg
     2821 gctgatctaa agtcgagcaa cggaacctat gtcaatggcg tgcgagcgca aaatggactg
     2881 atccgtcttg gagataaaat cagcgttcac gatgtcattt ttgatattat tcccgctccg
     2941 gatattcgtc ccacttcccc cgcagcggtc tcctccccac tgaaggggcc accaattcat
     3001 ggaggcaatg ccgctcctaa ctactattca cagcaggctc cgcaaatgat gggagatttg
     3061 aatccaggag tgccagctcc agagtcagtg gataagattt tgcctcctca aggggtgatg
     3121 caacgtctgc tgtttcaagt aaacgattat ctcgagagag tggctcttcc cggagtttac
     3181 aagctggccc agctcatgga attcaaatat attcttgggg gctttgtaat tctttttatt
     3241 ttcgcggtga ctttgctatc gttgataccc atggtgcaaa tcacccgctc gagtgttttg
     3301 gtggaaagta aacggagggc acagtcgatt gcgagaaatc tggctatctc caatcagcaa
     3361 atgcttcttc aaggtaatat atcttccctg agtactcatc tagcggaaac cgaagatgga
     3421 gtaaaggagg tcatgattat ccaagcttct gatggaatga ttgtggctcc ggcaagccgc
     3481 gcaggggtga ctccaaaaat tccgtttgtg catgccgctc gtcgtgaggg taaaacccaa
     3541 gttgaacaaa ttgatagtca aacgattggg gccagttttc ccattggcca atatgatcct
     3601 aatacgggag agccttctgt taaggcccat gcgattgtcc tctatgacat cggtagcctc
     3661 gcgtttgacg atggacatgt cttaagtctc tttttccaaa cattgatctt agcgtgtttc
     3721 gtgggtttgg gtctcttttt ctttatgtat aaattgatcg agtttccaat tatgagtctc
     3781 aataatcagt tggataccgc tcttcgtgag aaaaaagatt ccacggatgt caacttccaa
     3841 tttccggttc tgcaaaatct tgtcgggaat attaacagtt tactcagccg ttatacgcat
     3901 ggcggagacc acggcggtga gccgcggaat atggtcagcc acgacgtcga ggcggaaaat
     3961 ctcgtacaac tgatgggcta tccgacgatc gcgatcagcg gagaaaatcg catcatcagt
     4021 gtgaacggaa actttgagca gatcgcgcat tcgacgaggg cacagctaaa tcaccagact
     4081 ttgagtgcca ttcccgatgc ggcacttcaa cagaacttgc aacatctaat ccagaagtca
     4141 cgtgaggttc ccggcactat tcattcggac caattggagt ttagcggaca taactgccga
     4201 attcattgcc aagcaatggg tgtggaaagc agcacaatcg cttactttgt gatttgtata
     4261 acaccccttg aggggagtga ataatggcag ctcctcagct taagaacaca ttaaagtata
     4321 aaatcatgat ccagcgtgga cccttggccg gacagaatta ttcatttgat aaagaggtcg
     4381 tgcaaatcgg tcgtagccct gacaatgatc ttattttatc caatgacttg cgcgccagtc
     4441 ggaatcatgc agagattcgc tgggacggcg tggatttaag aataacaaat atcagttcta
     4501 aaaacttttt agctgtcaat ggcagccaag tcgaaagcgc gaagttggaa tcaggaacca
     4561 ttgtggtcgt cggagaaact gagttttata tcaatttaga gggtgtgtct gaaaaggaaa
     4621 ttgctgagcc taagaccaaa gtcaactttg tagaggctgt ggagaaagca gtgcctgggt
     4681 tcacacaaca catacctgtt cttcaacaga ctcattcaag tcccgtggac tttatacctc
     4741 cagtgaatcc caggggaatg cctgccagtg ctcatccttc gcagaatctt gccagatctt
     4801 ctcctcgtac gattaaaaaa gtttcgaatt caacactgcg tttttatgtt atcgtcgcag
     4861 tcataggtat cgcctttgcg tggctgatga gctccaacgt ggcaaaaaag aaagagctga
     4921 gctttcgttc tgctgaacaa atcgaaaaag atatagagtc ttctcgtgaa gagatgaagg
     4981 aattcgagac ccgaaaagag aaactggaca atgtgcaatt taggaaggct caagaaaatt
     5041 acatccgtgg gtttagagat tataagcaag gtaattttgg aagagctaag gaatcctttc
     5101 aagtggtttt gaatttggat cctgaaaatg aactggccac tcgctattat caattggcaa
     5161 agctgaaatt tgacgagttg gcaaagttcc atatgctgca gggtaatcgc tatcgagaaa
     5221 agaaaaattg gagaatgtgt aaatcaagtt attttaatgt catgacaatg gtcagtaata
     5281 atcaagatcc gctttttaaa gaggcgcggc aatactatga tcagtgttca ctggctcagg
     5341 agggcagata ctaatggctc gcctccgcgt gcgcttgcac ggcaaagtca tacaagaagt
     5401 ccagttatca ccagagcgtt cttatttagc gggccgccgc gaggactgtg atattgttct
     5461 ttccaatggg aaggcgattt ctcgcgagca ttttaaaatc cacttttcag aagatcagtg
     5521 gcagcttgaa gttctctctc ggttcggtga tgtcgtggtt aatggcgaaa aaattcaaca
     5581 agccattctc gagggtcaaa cgattattta cttaagaccc tatgaattcg aatattcgga
     5641 gatatcttca gacctggttt ctgtgcctcc ttcagtaaat acgccagagc ctttattcag
     5701 accggccttc gaggaagcgg atgaagtaga gaagactttc atcggctctg tggatatagt
     5761 gccttatatc aaggtgatta gtgaagtggg tgaagtcaaa gaactcttca aactcgaggg
     5821 cggcaacacc tgggtggccg gtcgcgatcc ttcatgtgat attatcattc gcgatcagcg
     5881 agtgagtcga cggcaatttg aagttcgccg ccaaggcaat ttatttcaga ttttggattt
     5941 aggcagcgtc aatggaactt tagtcaatgg taatcccgtt tcctccaccg atccaataaa
     6001 tataaaaagt ggcgatgcga ttggtgtttt ggacaaccat ctttattttg aattgcatga
     6061 tccggatttt aaggctcgca tggaattagt gaaaaccaac gttttaaatc cgctggttgc
     6121 tttgaatcac gaaggagagc cttccctcct cgtccaagat ccgcaacata tggaatctca
     6181 tctaggctat tcttatccag cgcatggaac ccctccccaa ccgaatactc aaaagaaatt
     6241 tgattttgag aagcatcggt tgaaactcat tgccggggcc gtgctcctga tagccgtggc
     6301 ctatattttt agtgatcaaa gcggcacaac tccgaaaacg acgcaaccgc cggctgaagg
     6361 tgtattaaat aaactttcac ccgagcagaa aattttaatc aagcaaacct atatgctcgc
     6421 aaagaatctc tatatgcagg ggaagtatga gcttgcgaaa gccgaagtga gcaagattta
     6481 cgaactggtt cctgagtatg aggatactaa agacattgaa cgtttagcga acgaagcttt
     6541 ggcgattcaa gaacagaaac gtcgccaaga ggaactagag aaatccaaag ctgaaactga
     6601 agaaaaaata caaaaagtcg cggacgagtg caaagcaaaa atcaacgccg attatactag
     6661 ctccgagctt gaagaatgct tgagttcggt cttgcaattc aatccggacc atcctctttt
     6721 tgctcaactt cgtcatcaag ttgaagagag gatgaataat aaaaaaatta aagaagcgca
     6781 aaaggcggag tatcaatcac aggttcaaaa acttcatggg ctgtttaata aagctgaagc
     6841 tagctctaaa gagaatcaat atctgcgtgc cctaaaagaa taccaaaccg tgagtacttc
     6901 tggtctgccg gacccccatg atctcaaagt ccaggcccgt aagcagatgg cagccattca
     6961 acaggttctt aaacagaaaa ctgatcaatt tgatcaagag gctgatgctg cttacaaaga
     7021 tcagaagcta aaagatgcca ttttagtttt aaggaaatct gcggatttaa atccaaacaa
     7081 caatgatacc aaagataaaa tctcgcgtta cattcttgag cttaagaaac aaatgatgat
     7141 cctttaccaa gagggtgtgc tggaagagag ctatggcaac gttgatggga gcgagaatcg
     7201 ccctggagcc aaagataaat ggaaaaaaat tatggacctg gacattcccg acggcgaata
     7261 ttacggcaaa gcgaaaataa agcttaaaaa atatggatta atctaatgag aatagaggag
     7321 cgttcttact caacaaaaat agttcgtcca aggcctgcga tttatattga gccggatggg
     7381 tcctttatcg tgatctcgac ttcttggggt gaccctgaac acgggcttaa agtgaacgat
     7441 gacatcgcga aatatattca agcagccaaa gccgacgtcg aagtcacctc tcccttcgag
     7501 tttttaactt ctctcagcgt cgaggccaat tatttaagaa ttgcgacctt aatctcgaac
     7561 gagtctcttt atcgcggcag caataagact gaatatcaag ccggcgtaga gacccttttg
     7621 cttcttcgta aaggttccct gatggcttac gctcaggtgg ggtcgcctca tcttctaatt
     7681 caaaagaaaa atcagttggt gactcctatt tcggtgggtt tcgaagctgt cttagagatg
     7741 agcggtgaag atcaagatct gctttctccc ctgcctcagt cccttcttgg aatcgatggc
     7801 aacgttaacg ttcgcacggg tgatttccgc atcgatgaga gtgatcgctt gcttctttat
     7861 gggggaagtg tttggccgaa gtccttatgg agtcctgact gcgattttca acaactcact
     7921 caaaagttag tgcaagcctc gccagagtct cctttttggc taggggtcgt gagtctcgaa
     7981 gattgaaatt ttcaaaaaat tcttttttac tcagtcatcg tcttgccagt tgctactgtt
     8041 ctgaagagat ttcatgaggt tatttcgttg tttttgaaac tcaggcagac tcagtaagag
     8101 atccacaagt tcaggactcg gttgaaattt atccggcgta tcccaaatca ggagggttgc
     8161 cagatttccc tgtttgatat tttggtccac caaaaaattt aaataggatc tttcaaaacg
     8221 acgtttctgt gtgcttttcg aaaagttacg ggcatcggct ctttgccagt caatgaaatc
     8281 taaaaagagg gcgttatcac attttttcaa atctactgag tctaatttcg gaatttccaa
     8341 aaggcgtaag caaaaatcct gaaacaggtt gaaatgaacg tttgtttcat cggctccatt
     8401 tttttcggcc accatttgcc aaaacggctg ttctgctttt tggctgatag ctttgaggct
     8461 attccaatct ttgagttcaa aggataaaag cgctgcccac ttcaaaatgt caggatcagg
     8521 gttcttgcca ttaattttga tctttacatc cttaaacaaa gtgtcttttt ggtccgctct
     8581 catccaacga gaaaaagcag gagtgtattt atttataaat tcttgatcat atccaatcca
     8641 aggctccgct tccactgcat ccaaagccgc aataaaacct tgccgctctt cttgggggag
     8701 ttcattgctt cggagtggat gaatttcttc gcgatcggtg agggtgttaa agatttgagg
     8761 atattgatca taaataaaaa gtatatgatt gctgagaact gcggatcgac gattgctgaa
     8821 accgcgccac tgtgaccagt cagattccaa gagaataaat cgattttcag cccctgccat
     8881 tggtttattt tgtacaagct gtgacaaaga ataaacagga aagatctccg caggtttgtt
     8941 tggatttttc ggagcaccta agagttcgta caggctttct cccaaatcca cgaggctgac
     9001 gttgcggtcg attttccatt gcaaggcttc atcatgggat ttccgcggag gtttgataaa
     9061 gagcgcgacc tgggtgtttt cgctatgaag attgctcgga ttcaattccc cgggacgatc
     9121 atcgctggcg tagccgttaa gtcccacaac aatgagggtg gtattgtccc aacgttgtat
     9181 agtcttaagc tccctaaaga aactataaag attgctattg agttcatcaa gttggctctc
     9241 atgggataaa tttcgtgctt caccgaattc attggtggtg acggtattgg taaagatcag
     9301 atccggaaca tataccattg cgaaaaaagg cgaggaatga cctacgtctt ggcgaatcca
     9361 ttgcagcaat gattttgtcg aagtcgtaaa aggtctatat aaagattgtt ttgaaggaat
     9421 aatgttatcc tcaaacaatt caaagccttg attaagtcct gactttctcc ataccggagg
     9481 tcccccagag aaaaaagccg tgcgatagtt tttctctaag gccgtttccg cgacggtgac
     9541 aaactgagaa tcaagccccg gttgcccatt gtggcgcacc cgatgttgaa aaggataaac
     9601 accggtcagg atactggtca aagtcggcac ggttaaaacg gaagggttaa aagcatgagt
     9661 gaagcgcaca gattctttac agagaatctc aaagccggat tttgtcgcat tcgtcatttc
     9721 ttcattacag agaatatcca aaacactgag gtcatcaacg gccacgataa gaatagaggg
     9781 cgtcgtggaa gattggcagg acaactggac tagacctgta gtcagtacca ataatattac
     9841 aataatagag gaggccttga ccgttctcat cggatttagt gataatgatt taattc
//
LOCUS       DATBTE010000014         1686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1777721, whole genome shotgun sequence.
ACCESSION   DATBTE010000014 DATBTE010000000
VERSION     DATBTE010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777721"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VIG33_00455"
     CDS             complement(<1..206)
                     /locus_tag="VIG33_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269330.1"
                     /translation="MLQEEILKINKFVKPEDHLTSHHGLEFFVDYTTDIRSRSFLSDH
                     AEKKAFRIAYIANSWLPDSETQRAL"
     gene            393..596
                     /locus_tag="VIG33_00460"
     CDS             393..596
                     /locus_tag="VIG33_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269331.1"
                     /translation="MKRALQQILTPLKGYEALVLACTHYPAVSAQIQKFLPSTQLLDP
                     AEATAAFVKSHGSGNGSYAVPEK"
     gene            835..>1686
                     /locus_tag="VIG33_00465"
     CDS             835..>1686
                     /locus_tag="VIG33_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269332.1"
                     /translation="MKYSQALAVGFLLFSGCTKVEKIIETRQASPNIRESFIQNNQKT
                     LSLKTSALGKVFMLVANSKTTGQQPRWIEHPIKLITFEKSGTQMGLFEVNYSQIYRSL
                     DSQKILQTFEIVSETSEQVTINIEKGFTVLNSVRSYEVMRPNKTEEAFKEDGSEVSAV
                     VKDLLVKSADVQNDKVHIIQFSRVQTPALLTVAQRRNKITQETPVLTDVENSYDTRID
                     IFPYSENKNFKPKEQDPEWTVGMFLSRILKEASSQSEALTTRWDTTQPIRYIIVGQPP
                     KEAIPSIK"
BASE COUNT          521 a          343 c          356 g          466 t
ORIGIN      
        1 agggctcgtt gcgtctcgga atctggaagc caagagtttg cgatataggc tatgcggaaa
       61 gcctttttct cggcgtggtc tgagaggaaa gaacggctac gaatgtcggt agtataatcc
      121 acgaaaaact ctagaccatg atgagaggtc agatgatcct caggttttac aaacttattt
      181 atcttgagaa tttcctcttg taacacggcc cagggggtat ccaaatcatt cgtgagtcaa
      241 aatttttaaa agttgcagtt attggcggac atcgtactat tttttcaaaa agctatagcg
      301 agccctttaa aaactcaaaa aagcgaattc gagggcgggt tgcgcagccc ctttccgctt
      361 tgattgaaaa gggagaaatc gagactccct caatgaaaag ggctttacaa cagattttga
      421 ctcctttgaa gggatatgaa gccttggttc tagcatgcac ccattatccg gcggtttcag
      481 cgcagattca gaaatttctt ccctccaccc aacttttgga ccccgcagag gcaaccgctg
      541 cttttgtgaa aagtcatgga agtggaaacg gaagttacgc agtcccggag aagtgacttt
      601 tattacttca ggaaatgcta ggcagatgaa aaaagcggcc cgtcttgctt ttcatgttaa
      661 aattcaaaaa atttattcta atgatgaatt gaagctgaat taaagatcct ttcaatagaa
      721 aactttccca agcctgaata aaattcaagg gccgagaatt tggcatagca acaaaagaga
      781 acgtgttaaa acgcgctctc gtttaaataa gattcacctt gggggaggat caccatgaaa
      841 tattcacagg ccttggctgt tggattttta ttattttctg gatgcacaaa agtcgaaaaa
      901 atcattgaga cacgacaggc ctctccgaat attcgtgagt ctttcattca aaataaccaa
      961 aaaactttgt ctttaaaaac atctgcattg ggcaaagtct tcatgctcgt ggccaactca
     1021 aaaaccaccg gccagcagcc ccgttggatt gaacatccta tcaagttgat cacttttgaa
     1081 aagtcaggga ctcagatggg actttttgag gtgaattatt ctcaaatcta tagatcctta
     1141 gattctcaaa agattttgca aacttttgaa atcgtttccg agacttctga gcaagtcact
     1201 atcaatattg aaaaagggtt cactgtctta aattcagttc gctcctatga ggtgatgcgt
     1261 cctaataaaa ctgaagaagc tttcaaagaa gatggcagcg aagtcagtgc tgtagttaaa
     1321 gatttattgg tgaagtcggc tgatgtccag aatgataaag ttcatattat ccaattttct
     1381 cgggtgcaaa cgcctgcttt gttaactgta gcccagcgac gaaataagat cactcaagaa
     1441 actcctgttt tgactgacgt tgaaaatagc tatgataccc gcattgatat ttttccttat
     1501 agtgaaaata aaaattttaa gccaaaagaa caggatcccg aatggacagt ggggatgttc
     1561 ctttctcgaa ttctgaaaga agccagcagt caatcggagg ccttaaccac ccgttgggac
     1621 accactcaac ccatccgtta tataattgta ggccagccgc caaaagaagc gattccttct
     1681 attaaa
//
LOCUS       DATBTE010000015         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1982635, whole genome shotgun sequence.
ACCESSION   DATBTE010000015 DATBTE010000000
VERSION     DATBTE010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1982635"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VIG33_00470"
     CDS             <1..746
                     /locus_tag="VIG33_00470"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HEY8269333.1"
                     /translation="NHRIERNQISGHRDGVYLEFATGTLIRENLVKQNIRYGLHFMSS
                     SNTQYHGNIFTENGAGVAVMYSRQIKMHDNKFTGNTGPAAYGILLKEVHESEIFNNEF
                     KKNTVGIYMEGSNRSLFRSNDISENGWALRIMGDCENNLFTQNNFVGNTFDVTTNSDH
                     NWNSLNENYWSHYDGYDLNRDGYGDRPYRVVSLSSVILEKVDSSYILMNSFLFNLMDQ
                     VERALPSLIPESMKDEKVLMTPAKVGVRL"
     gene            760..1515
                     /locus_tag="VIG33_00475"
     CDS             760..1515
                     /locus_tag="VIG33_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8269334.1"
                     /translation="MMNQAMIEIKNLYKAYRMQSALAGVTLSVRRGTITGLVGPNGCG
                     KTTLIKSILGLVVPDEGEVRVADKSISEGPLYRELIGYMPQNPDFPSNLSIYELLEML
                     EDVRGRRAPNKHDLLKLFDLDSQQTKTFGTLSGGTKQKVAAVAAFMFTAPILILDEPT
                     VGLDPISANRLKALAKSHAKEGAAILLVSHIMSEMEHLVDQMTFLLEGKVSFSGSTSE
                     LLLRAQASSLENAVVKLIGQRMEDPQGVNEAVK"
     gene            1512..2294
                     /locus_tag="VIG33_00480"
     CDS             1512..2294
                     /locus_tag="VIG33_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8269335.1"
                     /translation="MSKSLQKILKYEFLNIFRDRWIFVYTAIVAVTIFSVQKICGEFQ
                     KTVLTLSSLSVVFVPLITSFFTTLYWYYSDRFTQLMATQPVKRSHIFLARYGALTFSL
                     SACYVVGTVLGAALSGSFSLGLVVLIAMNVLLTFIFIALSMLISAQVEDRMRGVGLVF
                     GLWLYFVLIHDGLLLLGLVALREYPMDILGGVLGSLSPIGLSRVVLLMFNEGSLLLGH
                     TGALVREIMTSWQGYLMAIGISVVWAIVPLLFGVRSFKKKDF"
     gene            complement(2291..>2618)
                     /locus_tag="VIG33_00485"
     CDS             complement(2291..>2618)
                     /locus_tag="VIG33_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HEY8269336.1"
                     /translation="KNGELRILLCDDAPLNRFKPSVDYLFNSIAELSICKNTHAAILT
                     GMGEDGARGMLKMKKNGAYTIAQSEDSCVVFGMPKAAIEFNAINDILDIDAMTEALLG
                     ARPKCA"
BASE COUNT          663 a          490 c          637 g          828 t
ORIGIN      
        1 gcaatcatag gattgaacgt aatcaaatct ccggtcatcg tgatggagtt tacttggagt
       61 ttgccacagg aactctgatc cgtgaaaatc tggttaagca aaacatccgg tatggattgc
      121 atttcatgtc ctcaagtaat actcagtatc acggaaatat tttcactgag aatggagctg
      181 gagtcgctgt gatgtattca cgccaaatta aaatgcacga taataaattt actgggaata
      241 cgggacctgc agcttatggt attttattga aagaagttca cgagtcagag atttttaata
      301 atgaatttaa aaaaaacacc gtaggtattt acatggaggg ttcgaatcgc tccctctttc
      361 gaagtaacga catttcggag aatggttggg ctttaaggat tatgggcgat tgtgaaaaca
      421 acttgtttac gcaaaataat ttcgtcggca atacttttga cgtgaccacg aattctgatc
      481 ataactggaa cagcttaaat gaaaattatt ggagccatta tgatggttat gatttaaacc
      541 gggatggcta cggcgatagg ccttaccgtg tggtcagtct ttcttccgtg attttagaaa
      601 aagtcgattc ctcttacatt ttaatgaata gctttttatt caacttaatg gaccaagtcg
      661 agcgggctct tccaagtctg ataccagagt ccatgaaaga tgaaaaggtc ttgatgactc
      721 cagcaaaagt cggagtgcgt ttatgaaaag tgcagctata tgatgaacca agctatgatt
      781 gaaattaaaa atctatataa agcttatcga atgcaatccg cgctagcggg ggttacgctc
      841 tctgtaaggc gtgggactat tactggactt gtggggccta atggttgtgg caaaaccacc
      901 ttgattaaat ccattctagg cttggtggtt cctgatgaag gggaagtcag ggttgccgat
      961 aagtcgattt ctgaaggtcc tctctaccgt gaactcatag ggtacatgcc tcaaaatccg
     1021 gatttccctt ctaatttatc tatttatgag ttgttggaaa tgcttgagga tgtgcgcgga
     1081 aggcgcgctc ccaataagca cgatcttttg aaattatttg atctcgattc tcagcagaca
     1141 aagacatttg ggacgctttc cggtgggaca aaacagaaag tggcggctgt ggctgcgttt
     1201 atgttcacgg cgcctatttt gattttggat gaacccacgg tgggtcttga tcctatttct
     1261 gcaaatcgat taaaggccct ggccaaatct cacgcaaagg aaggggcggc tattttgttg
     1321 gtttctcata tcatgagtga gatggaacat ctggtggatc aaatgacttt tttattagaa
     1381 ggcaaagtta gtttttctgg ctctaccagc gagttgttgc tcagagcaca agcctctagt
     1441 cttgaaaatg cggtggtgaa gttgataggg cagcgaatgg aagatcctca aggcgtaaat
     1501 gaggcggtta aatgagtaag agccttcaaa agattcttaa gtatgagttt ctgaatattt
     1561 ttcgagatcg ttggatattt gtatataccg cgattgtggc ggtcacaata ttctctgtac
     1621 aaaaaatttg tggcgagttt cagaaaacgg ttttgaccct gtctagtttg tctgtggtct
     1681 ttgtcccttt gatcacgtca tttttcacca ctctttattg gtattatagt gatcgcttta
     1741 cgcaattgat ggcgacgcag cccgtgaaac gctcacatat ttttttagcg cgttatgggg
     1801 cgctcacatt ttctttgtcg gcttgctacg tggtaggcac ggtgctgggg gcggcattga
     1861 gtgggagttt ctctttaggt ttggttgtat tgattgcaat gaatgtgctt ttgaccttta
     1921 tatttatcgc cctgtcaatg ttgatatcgg cccaggttga ggatcggatg cgtggcgtgg
     1981 gccttgtctt cggcctttgg ctttactttg tattaatcca cgacggtcta ttgttgttgg
     2041 gtttggtcgc cttgcgtgaa tatcccatgg atatcctagg aggtgttcta ggctcacttt
     2101 ctcccattgg tttaagcagg gtggttttat tgatgttcaa tgaaggctct ttgcttttag
     2161 gccatactgg cgcattagtg cgcgaaatca tgaccagttg gcaggggtat ttaatggcca
     2221 ttggtatttc tgtcgtctgg gctattgtgc ctctattatt tggggtgaga tcatttaaga
     2281 aaaaggattt ttaagcgcac ttaggtctcg ctcccaacaa cgcctcggtc attgcatcaa
     2341 tatctaagat gtcattaatg gcattaaatt cgatggcggc ttttggcatt ccaaagacga
     2401 cgcaactgtc ctcgctttgg gctatggtgt aggcgccatt ttttttcatt tttaacattc
     2461 ctcgagcccc atcctctccc attccagtaa ggatggccgc gtgggtattc ttgcaaatag
     2521 ataattcagc gatggagtta aataagtaat ccacagaagg tttgaaacga tttaaagggg
     2581 catcgtcgca taataaaatg cgcagctctc cgttcttt
//
LOCUS       DATBTE010000016         4490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_206903, whole genome shotgun sequence.
ACCESSION   DATBTE010000016 DATBTE010000000
VERSION     DATBTE010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206903"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..513
                     /locus_tag="VIG33_00490"
     CDS             91..513
                     /locus_tag="VIG33_00490"
                     /inference="COORDINATES: protein motif:HMM:NF044286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269337.1"
                     /translation="MDPIITTAIVAALANLSNDVIKDSYDALKAGLKKMFGEESDLID
                     ALDKLEKKPNSEARKATVQEEVEIAKVNDDPEILKLAKDLLDKIKEQPSGQEIINQTQ
                     NNTVSGVNVGGNFEFKPVQEDKRSWKPDPGRGTGKTDR"
     gene            597..4049
                     /locus_tag="VIG33_00495"
     CDS             597..4049
                     /locus_tag="VIG33_00495"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8269338.1"
                     /translation="MSQPIPDHQSQEQNSVIKDTNISGDLTFAPVQIGTKIETLIVES
                     SLEKITQRKLNENSPYQGLKRFNLKDRERFFGRDKLIARLFEAVNRSNLSLVLGASGS
                     GKSSVVRAGLIPELKKSLKYQTFYDFIFTPNQDPFDSLYRCLLSEEKDYNFSKSEAEI
                     ALEAKAETLSKVISTLKKDEERWLIFVDQFEELFTICDDPDKRKNFISGLVQVAKSGN
                     SSVKLVLAMRSDFLEQFSFYPDLGSIANQNNIHLVTEMYPDELQQAIEQPAAMHGVVF
                     EEGLVKQIIDEVEGQKGYLPLLQYTLDLFWESECRTLDADGHPNIENRTLNKKNYTAL
                     EGVRGALQKRVNEIYKDVCERNKDGEAATKQIFLKLVNIVDSDSGSRAVNRRAYRYEF
                     VGEPVENTLKRFVDENLLVSSYEYSNEKELLIGNNKKNLENATIEIAHEILLSSWDRL
                     KRWLEEEKEAIILKNWLAGETRRWLEVRAKDESKANDELLKGARLDQTVEFREKNAFE
                     KLGGLVQEENKFIDASVEWRESARSEKIHLQRRAIKWLSGGLTAALIAMGIATWQWQR
                     SENLRLATQSEALGSTAVQQFDSGRQIEALLLAMQSGLMLKDLIQDSRSLNNHYPAVS
                     PLFALQTILDDIRERNRLEYPGGSELVVNSQYVSRVSFSSDGKYIVRIPEHGSEEHWD
                     LSGKLINQSVQPNHDTTSNKEPSSIEECVVPLGGDRVIRLRYLQERPLEQQLTEQLAQ
                     LTGQSEGILSIRCSPNGKYVVITGRDGTVRLLDLSTKQIAKIIGRQGGVMDIRFSPDG
                     QNFATWGGNVLFWELPKNQMAQHMTLLNGNQGSVTAISFSQDGKYIATAGSNGTVNLW
                     DSSGNKIRPELKIGEGIMGVKFNRDGKKIVIVTDQGTIQIWDLAGNPIQTVLEKPQNQ
                     RLNVINVSFSPDGNYIAMAMAGGGVELRKITGEQLPGRFTGHQGWIMGMNFSVDEKLL
                     ATAGDGDTIRLWKLSGEQVKEIRAHQGETMSVSLSPDGNLVASAGNDGTIRLWDLQSK
                     EEIAKFAGHQGKVLGVSFSPDGNFLATAGTDGTIRLWTLSGEQMAQFIGYSSGLSSEA
                     TGGAFGSLGMTFSPDGRQIATAEDNGGARLWRIETLDELLYRGCEWLKDYLAAHPDAP
                     KVCS"
BASE COUNT         1435 a          790 c         1090 g         1175 t
ORIGIN      
        1 attgtgtatt tagtttgaag tgttattgtt tttttaatgg ttgaatatgg aatggtgctt
       61 tttaatcaat tggtttagag aggatgaaat atggatccga ttattactac agccattgtg
      121 gctgctctgg ctaacctgag caacgatgtg atcaaagaca gttatgacgc gctcaaagct
      181 ggtttaaaga aaatgtttgg cgaggagagc gatcttatcg atgccctcga taaattagaa
      241 aaaaagccaa actcagaagc tcgcaaagcc acagtacaag aagaagttga aatcgctaag
      301 gtcaatgacg atcctgagat tctaaagtta gctaaagatt tactggataa gattaaagaa
      361 caacctagtg ggcaggagat aatcaatcaa acgcaaaaca acacggtgag tggcgtaaat
      421 gttggtggaa actttgaatt taagccagtc caagaagaca aaaggagttg gaagcccgat
      481 cctggtcgag gcactggaaa aacagaccgt tgaaacattt aaacgatgat tagagaaaat
      541 actggattta gctgaaccga cagcataagc agccaataca ttgggatatc attactatga
      601 gtcagccaat tccagaccac caatctcagg aacaaaatag cgttattaaa gatactaaca
      661 ttagcggcga ccttaccttc gctcccgttc agattggcac caagattgag actctgatcg
      721 tcgagtcgtc gcttgagaag attacgcagc gaaaactgaa tgagaattca ccttatcaag
      781 gactgaaaag atttaatctc aaagacagag agcgattttt cggtagagat aagttaatcg
      841 cgagattatt cgaggcggtg aatcggagca acttgagttt agtgttagga gcctctggaa
      901 gtgggaaatc ttcagtcgtg cgcgcgggat taattcctga gttgaagaag tcactgaaat
      961 atcaaacatt ttatgacttc atctttacgc ctaatcaaga tccctttgat tccctatatc
     1021 ggtgtttact tagcgaggaa aaggattata acttcagtaa atcagaagcc gagattgcct
     1081 tagaagcaaa agccgagacg ctaagtaaag tgattagcac cttaaagaaa gatgaggaac
     1141 ggtggctaat ttttgtcgat caatttgagg aattgttcac tatttgtgac gatccagaca
     1201 aacgcaaaaa ctttatttca ggtcttgtcc aggttgccaa gtctgggaat agttctgtca
     1261 agcttgtact ggcaatgcgc tctgactttc tggagcaatt tagtttctat cccgatttag
     1321 gttccattgc caatcaaaat aatattcatc tggtgactga gatgtatcca gatgaactgc
     1381 aacaggcgat cgagcagcca gcggctatgc atggtgtcgt gtttgaagaa gggttggtta
     1441 aacaaattat tgatgaggtc gagggacaaa aaggatatct tcccttatta caatataccc
     1501 ttgatttgtt ctgggaaagt gagtgcagaa ctctcgatgc tgatggtcat cctaatattg
     1561 aaaaccgcac cttaaacaag aaaaattata cagctttgga gggggtaaga ggggcgctgc
     1621 aaaagcgggt caatgagata tataaagatg tatgtgaaag aaataaagat ggagaagctg
     1681 ccactaagca aatatttttg aagttagtaa atattgttga ttccgattct gggagtagag
     1741 cagttaatcg gcgagcttat cgctatgaat ttgtcggtga accagtagaa aataccctta
     1801 aaaggtttgt tgatgaaaat ttactagtga gtagttatga gtattcaaat gaaaaagaac
     1861 tattgattgg gaataataaa aaaaatctag aaaatgcaac tattgaaata gcccatgaaa
     1921 ttttgctctc gtcctgggat agattaaaac gctggctgga agaagaaaag gaagcgatta
     1981 ttctcaaaaa ttggttagct ggtgagacaa gaagatggtt agaagttcga gccaaagatg
     2041 aatctaaagc taatgatgaa cttttaaagg gcgctagatt agatcagaca gtagaatttc
     2101 gagaaaaaaa cgcctttgaa aagttgggag gcttagttca agaagaaaat aagttcattg
     2161 atgccagtgt tgaatggcgc gagagcgccc gatcagaaaa aattcatctc cagcgacgtg
     2221 ctattaaatg gctttccgga ggtctaacag cagccttaat tgcaatgggt attgcaactt
     2281 ggcagtggca aaggtctgaa aatctgcgtt tagcgactca atctgaagca ttgggctcta
     2341 ctgccgttca acaatttgat tctggtagac aaatagaagc tttgctatta gctatgcaaa
     2401 gtggattaat gctcaaagat cttattcaag atagccgctc tctcaataat cactatccag
     2461 cagttagccc cctcttcgca ttgcaaacaa ttcttgatga cattcgtgaa agaaatcgat
     2521 tggaatatcc aggtgggagc gaactcgtcg taaatagtca atatgtttct agggtgagtt
     2581 ttagttcaga tggaaaatat attgttagga ttccagagca tggctcggaa gaacactggg
     2641 atctatcagg aaaattgatt aaccaatcag tacagccaaa tcatgatact acttcaaata
     2701 aagagcctag ctcaatagaa gagtgtgttg ttccattggg aggcgaccga gtaatacgcc
     2761 ttcggtattt acaagagcga ccattggagc aacagttgac tgagcagtta gcccagctca
     2821 ctggtcagtc agaaggaatc ttgagcatac gctgtagtcc taatggcaag tatgttgtta
     2881 taaccggaag ggatggtact gtaaggctct tggatttatc tacgaaacaa atagctaaaa
     2941 ttattggtcg tcagggcgga gttatggaca taaggtttag tccagatggg cagaactttg
     3001 ctacatgggg gggaaatgtt ctattttggg agctgccgaa aaatcagatg gctcagcata
     3061 tgactctatt aaacggaaac cagggttcag ttacagcaat aagcttcagt caagacggaa
     3121 agtatattgc tacagcagga tcaaacggca cagtaaatct ttgggattcg tcaggaaata
     3181 aaattaggcc agaattaaag attggtgaag ggatcatggg ggtcaaattt aaccgagatg
     3241 ggaagaaaat tgtcatagtg acagatcaag gcacaataca aatttgggat ttggcaggaa
     3301 atccaattca gactgtacta gaaaagcctc aaaatcagcg tcttaatgtt attaatgtga
     3361 gttttagtcc agacggtaac tacatcgcta tggcaatggc tggtggtggt gtggaactta
     3421 gaaaaatcac aggggaacaa ctacctggtc gctttactgg tcatcagggc tggattatgg
     3481 gtatgaattt tagtgtggat gaaaaactcc ttgcgacggc aggagatggt gacaccatcc
     3541 ggctgtggaa actttcaggt gaacaagtca aagaaatacg tgctcatcaa ggagagacga
     3601 tgagtgtaag tttaagtccg gatggaaacc ttgtagcttc agcaggaaat gatggcacaa
     3661 tccgcctctg ggatcttcaa tcaaaagagg aaatagccaa atttgcaggg catcagggca
     3721 aagttttagg cgtaagtttt agcccagatg gaaatttcct tgctacagca gggacagacg
     3781 gcactattcg actttggact ctctcaggag agcagatggc tcaattcatt ggttactcta
     3841 gtggacttag ctcagaagct acaggtggtg catttggcag ccttggaatg acttttagcc
     3901 ctgatggacg acaaatcgct actgccgaag ataatggggg agccagatta tggcgtattg
     3961 aaacattaga cgagctattg tatcggggat gtgaatggct caaggattac cttgccgctc
     4021 accctgatgc accaaaggta tgttcataaa tgtcatgacc aaatgtgggt tcgcgcataa
     4081 ttaggagagc gaaaaaattg ggtagcaggt tgggtagtta tgatgggtac gctaaccgta
     4141 tacacccact agaattcata tagagccgca gactacacat aatcatggat aacgaagaga
     4201 acagagctga cgtcgatgcc taatctttca accaactgga tcgtctccac gcttcgcgtt
     4261 ccggcggcag tttaacgcaa atgttgaata gctggtttca aagaccttgc agacattcat
     4321 gaggaattgt tgacaggcag gttatggccc aggctgatga catggactcc tcctcaccct
     4381 cagggtgtca taatgcaccc agccaattaa cgggaaaaag aggagtccga aatgaacagt
     4441 aatatagtgg gtttggattt agccaaaagc atttttcatc tctatagcct
//
LOCUS       DATBTE010000017         1945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2260, whole genome shotgun sequence.
ACCESSION   DATBTE010000017 DATBTE010000000
VERSION     DATBTE010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2260"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VIG33_00500"
     CDS             complement(<1..359)
                     /locus_tag="VIG33_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269339.1"
                     /translation="MKPHNTAANGKIMQKIPVVPLLKSILLMGGTAVFGAYALGSLLQ
                     SEKPVAHKIIDMHTHVACLGSAHKSDGECYISPEMQKSFKFSIYLKSFGVSVDELKRT
                     ENLAVFDRLIEKIQSSKR"
     gene            complement(731..934)
                     /locus_tag="VIG33_00505"
     CDS             complement(731..934)
                     /locus_tag="VIG33_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269340.1"
                     /translation="MNWVLNAFFILSAMAADLHKELRIDAYNLHQALNYMNVYTSGKC
                     EAALDLAKKHLSQIKEKSNKAVW"
     gene            complement(1248..1373)
                     /locus_tag="VIG33_00510"
     CDS             complement(1248..1373)
                     /locus_tag="VIG33_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269341.1"
                     /translation="MRFLAAWFPNPGEYWMNIVILILFIAASVWGLRKIQSKRRQ"
     gene            1542..1739
                     /locus_tag="VIG33_00515"
     CDS             1542..1739
                     /locus_tag="VIG33_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269342.1"
                     /translation="MEGQNPMTEKPTALKDYAAFRALLKNFFKEADKAIQAEIIKRLV
                     GKIEVGVDFVRVHYHTTEIHC"
BASE COUNT          558 a          394 c          412 g          581 t
ORIGIN      
        1 cgtttcgaag attgaatttt ttcaatcaaa cgatcaaaca ctgctaaatt ctcggttctt
       61 ttgagctcgt caacgctgac gccaaaagat ttaagataaa tcgaaaattt aaaacttttt
      121 tgcatttccg gcgaaatata acattcgcca tcgcttttat gagcactgcc caggcaggcg
      181 acatgagtgt gcatatcaat aattttatgg gccacaggtt tctcagattg gagcaagcta
      241 ccaagggcgt aagcgccgaa gacggccgtg ccgcccataa gtaaaatgga cttcagaaga
      301 ggaacgacgg ggatcttttg cataattttt ccgttcgcag ctgtgttatg aggtttcaag
      361 ttgttctctc gggcatagca cgaggaactt ggtatgtaaa ggtacgctaa aaggacctga
      421 gctagatgtg gttttttgag tcgagatttt ctcggagctt tctaaaatcg tcaatataat
      481 aatttgcgac ccttgctcaa gttctattcc ttaatcttta gggggagttt taggctaacg
      541 aagggtttga tgatcgcatt attctacgcg aggtgcagag gggcagatgg tctgaatttc
      601 actgtgccaa gccaactggt gctcaatttt atttatctcc aacagttcac cgtatttcat
      661 tctgagtaac tccagatcag ttacatcttt gattgttggt ttattgtatt tctggagggc
      721 tttagttatc tcaccaaact gctttattag atttctcttt aatttggcta agatgttttt
      781 tggcgagatc aagagctgct tcacacttac ccgaagtata gacattcata taatttaggg
      841 cttgatgtag attgtaggca tcaatcctta attccttatg taagtctgcg gccatcgctg
      901 ataaaataaa aaatgcattt aatacccaat tcatgtctta tgatcacatt tattaaatga
      961 agatgtctac aaaactaatg ataagcagct aaacgccgcc catcaccgaa ttgattttga
     1021 gaccagatga attttaaatt ttgaatgtca ttttggaaag actggaagtg ctttttcgaa
     1081 aagatgctca agaacacgag ctttgccagt caaagctttt gaaggatcgc cacctgtatc
     1141 aatccttttc gaacggcttt cgagaaagct gattattgac ctgcgagttt tagatcgagg
     1201 ccggtagact caccggccct tagagaagga gttttatagc gatgaaatta ttgtcgacgc
     1261 tttgactgga tttttcggag accccaaacg gaagctgcaa taaataaaat tagaatgacg
     1321 atattcatcc aatactcacc gggattcgga aaccatgcag ctaagaatct catagtaacc
     1381 tccgtctaaa ccatcgttat aatttagcaa cctacttaaa gtcattaaag aaacgtttca
     1441 aagatagaac ttaagtattc agagaagcga aagatgacgg ctagcaccgc tagaaataag
     1501 actgccctaa aacggacagg cttatcacct aaggtcatta gatggaaggc caaaatccga
     1561 tgacggaaaa accgacggct ctaaaggatt atgcggcatt ccgtgctctt ctaaagaatt
     1621 tctttaagga ggctgataaa gccattcaag ctgaaataat taagcggctt gtcggtaaaa
     1681 ttgaggtcgg cgttgatttt gtaagagttc attatcacac gactgaaatc cattgctgat
     1741 cctttgtctc tagcttcctg ataactcgtg tttacagaag ttcacattgt tatggccgat
     1801 tatcctatct ggcccgcatg tactttagcg gcgccggtac tgtggcaatt tgattggtct
     1861 cgtcgccgat ccccaaccgc cacaggattt acttggtgga atgacctcct tccaagcaga
     1921 aatacattcc ctgggttcct tccgg
//
LOCUS       DATBTE010000018        17090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1846596, whole genome shotgun sequence.
ACCESSION   DATBTE010000018 DATBTE010000000
VERSION     DATBTE010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 17090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17090
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1846596"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="VIG33_00520"
     CDS             complement(<1..1227)
                     /locus_tag="VIG33_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269343.1"
                     /translation="MRKPSSSLLLFIIISVITVITSMASAEKEGILSKLLAPGPLIKG
                     HQDLENKDCLKCHDAGKGVPNEKCLECHKPIRPFVTQKRGLHGLTTKTCMECHRDHQG
                     RNFDSIVVDKDNFEHGKLTGYFLEGKHAEIKCEECHKEKLGPKSVRPGDIRYIGKATS
                     CISCHRKDDVHFFKNEFAKKDCNACHTLKTWKDNIKFDHSRDANYKLVGKHSEIKCND
                     CHLADKKRKIFRYRWPQLKTALCLSCHEETHKANLSHRFTGGKCTTCHTEDSWKIENF
                     NHKITGYKLGGRHAELKCTECHKQRPAVLKETSTKNYKWLGLKTQCLTCHDDFHRFGT
                     VKNPHFGSLNNCSACHTDKVWKPAINFDHNTHTRYVIDGAHLEVKCKDCHFDKVTKQP
                     IYKYPKLVEKNCENCHK"
     gene            complement(1224..2564)
                     /locus_tag="VIG33_00525"
     CDS             complement(1224..2564)
                     /locus_tag="VIG33_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269344.1"
                     /translation="MKLIKVFSIFLIIASLFCKENKALAAKSYGRFVFGGFTATEKFT
                     TDAFGSDSNDEMVFSSRLFYKVTNMGEGNWEFVSDLRDKHDFFDKLDRERLQLTDRNE
                     FQARQINTRWVNPGGQLSLSLGRFPVQDVGAIYTDGVLAEYRWTPSLWSSFFGGKNPQ
                     AESHSYLTFNPQANIAGAGLTYQSRTGGWARNFYLTNGVVSESYGNETDRSFLFHEMN
                     YQWEENSRILSLMYFDFVPRSYIQNGSLIWQQEYTENLSSELGILAMDSIEYYRHQGI
                     RETLPPSPYQEAQAKFSIGSPRSSRWTLGTISGHRAYDQKNKTEVSLSYFQSRVFAPN
                     WDLYAKGGNRNNFVSTDTFFRTGAGYFSRKWEFSLDVGYATEKHEDGTLLHPLEIEFS
                     VSNSISRTLFWTGSIQRATDESVTILTSFFKLGYRFGEAETAPRRDAAPLRGPI"
     gene            2616..4496
                     /locus_tag="VIG33_00530"
     CDS             2616..4496
                     /locus_tag="VIG33_00530"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEY8269345.1"
                     /translation="MGLANVVSRLNKNKFFRFISSVRLAVPTMLILCVLVGWGTILES
                     LYNAEYASLRVYKSWWFSIFMLILWLNIFCAPLARYPFKKHQIGFVVTHIGLLILLAG
                     GLVTNRLGIDGQLQVMEGTSNSSVMLSRLMVGYQTEGSPSIQKIVFDRSINSKDSSNL
                     DFINKELGAWFKVNQYLPFAKVEKNYESSPVTSDETSISFIMKSQFFNVTEWLSTQDE
                     PEKQMGPATLRIVKGEYVAANNSRKTEKKTNKKSEKKESLKKRTTSAVSGAEMVQILD
                     AQTEKEIQTVPLAKLLNTPVVVNGVTITVSKKLNNAIVAANKLAEGDPSGSNPALEFE
                     LKKGGETKREVSYVKHATFSLHPQGAFGLRLQYLGGSGGSAIGPAMSEENIQENSEPM
                     AMQRPGGNVIEFHIPSGNQNNNQKVQVVLFKNGEFVQEAFVGPGESVQTPWMGMQLFI
                     GSVIHNSQSAVVARPVALEKKSNLPPSALELNVDAASPSLWLSEGDEKEIMVGNKRIH
                     VFYGRENLTLPFEIALKKFSKIDYPGTETPMSYESLVQINGHGPVTKISMNEPLKVEG
                     YTIYQASYSLQPNGPPVSVFSVNKDPGRFWKYLGSIILAIGVILYTLMKSRLYRKEKR
                     GV"
     gene            4513..6186
                     /gene="ccsA"
                     /locus_tag="VIG33_00535"
     CDS             4513..6186
                     /gene="ccsA"
                     /locus_tag="VIG33_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY8269346.1"
                     /translation="MKLTLVLALEIFFTAAVYAQHEGMNPKVESATPEKAMMDLKIQG
                     EQIQKDPIRSLMVHQNGRLKPFDTFAQESILFLNGSYKRTGLHPVQTYLALMTSSTAP
                     YVELVEVRDPELRVQMGFMKSKRFMSLADLESSNISSLTEPLFKKQEENSKSLTSAEK
                     TVLETFNQAMLLQAVIRGDHLARAADFSFLKGTHGEEMAASEVQAALREYIQCLMSSE
                     CSQGIKAEALLAVSGKQETPDMFKHYFEKTKAEIFYNDFRPFFWASLFYILLGVLFSL
                     SFIRNKIGTKWLSLLLVLPLSLHILGLGLRVYITQFAPVTNMYGTMIWVSLGVTVFST
                     LLFILYKNAYVLCAALICSGLILMLTEQIPMILSPDLDPIVAVLRSNFWLSTHVTTIT
                     ISYAAFTVAAVLGNIALFRVWIHKENDAFFKEYSHYAYRMIQLGCFLLTVGIILGGVW
                     ADYSWGRFWGWDPKETWALIADVGFLAILHARIAGWLKPFTLLAFSPVAYLLVIMAWY
                     GVNFILAAGLHSYGFSSGGALVVAIYVVVQAVVLALGFWRYFSLKKVAA"
     gene            complement(6614..7462)
                     /locus_tag="VIG33_00540"
     CDS             complement(6614..7462)
                     /locus_tag="VIG33_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269347.1"
                     /translation="MRFKSVMFIWILFLALLSYEYYGLNFNESLSLLKKLQAMGYLPA
                     RYQINKEPGRQLSLFLGWTGLGLMIMMNVYSLRKRLSWLSHSGKLTSWLNFHILCGLI
                     GPTFILFHCNFKVRGVVGISFWSMVISFSSGLIGRYFYIQMLQAKKEFEKEAEQRLAK
                     IGRMLARINVPFTQQDQELYSKKALQLAGAPLTDTPYNPLKALVYSMAGDIRMAFTEP
                     EIGPQWPTATKFILKDYAVNKRRSALLAPFQRLMGYWHAFHFPFAIFMYVAAIIHVTA
                     ALMFGV"
     gene            complement(7452..8792)
                     /locus_tag="VIG33_00545"
     CDS             complement(7452..8792)
                     /locus_tag="VIG33_00545"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEY8269348.1"
                     /translation="MGFLNFIADNVVLTFIVLVGFYSMLSILRREKHHREAKNLLSKA
                     VKSGLNEPMSLHPEIDSGLCAGCGACTTICPEGDIIKLINHKAVLVGPTKCVGHGECE
                     KACPLNAIKLVFGTKTRGMDIPRLNSNYETNVPGLYIAGELGGMGLIRNAIKQGILAT
                     TDAVKKLPQENAEYDVIIVGGGPAGLASGVTAIAEKKSYLLLEQNSFGGTVYNFPRQK
                     IVMSHPFELPLIGKFKFESNRISKEQLLKYWNNVRKKTGLKVQVDTKFEGLEKTGNIF
                     KVKSSKGEYTARKVILCMGVRGSPRRLGVPGEDLPKVTYNLLDPEQYQGKKVIVVGGG
                     NAAVEAAQMLSEKRWRNEVHLLVRGPTFDRCNEDNMKRILEMESKGLLSIWYNSSVQA
                     IALGLTKIKKQEEIIDLRNDFVFIFAGAELPHKFLMGLGVEIDKKFGEGMKNAI"
     gene            complement(8877..12209)
                     /locus_tag="VIG33_00550"
     CDS             complement(8877..12209)
                     /locus_tag="VIG33_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEY8269349.1"
                     /translation="MRQLKILLPFAALGTAILMFQNCGVERPSVENAEYASVSFKHTG
                     QETSCSTCHELSRPNTTVGFIGLNPNTPFDYFSHAAGTDCITCHHPQTLSRTSADWAG
                     GKYVHSPLLTSCVSCHSSQRPTTIVNGFNHSTSGTGECFSCHQPALQSSFSSLSDWSG
                     AGGTPATLVWDPSADINIVAQIPTYLNTSIQSLTSQNQSYHMAMNHKTTQLKATDLQN
                     CALCHAGIASGNYLPGEFHPSLTKASLGQPTSCSDCHTSGAVRPKGFVGHSDSSRIPP
                     SKVMKHDAVVWAKNAAGNYVATTTPIVNSDCSTCHKQPGTAWQSAQFHQSLVGAGLTQ
                     PSSCVDCHANSRPVGPVPVTAAAGTTKFDHVNLGGTGDCLTCHTSQSTWGGGVFHAAN
                     GKPASCIACHESSRPNTNSSFTASNPNTPFDFNTHGSPLDCATCHTATTQFTAKSQWS
                     GGNFVHSSSLTSCSSCHSSQRPTGIINGFDHSKSGTGDCIGCHQQVLTKNQFTSLANW
                     GSATTFPSGYVGAGSLSVTSTHLNYSGTRVISTSQQTQSLPKQMLHTSTQVPASIAND
                     CATCHNQASSGVYAGGKFHASLTVKGLAQPTSCRDCHTGTLPVDIVGTALKPMNHAAA
                     LSGGGTAVSNFDCATCHAQPGVTFADGKFHFKISSKSPTNCTTCHYPIMNQTLINKMA
                     HTSNFAVQDCLACHAQPTSAQATNPASTNWAGGLYHGKITTPPTACIDCHAGQKPSGV
                     TVSTTDSQHMSHISTNLGTECATCHLNDLNTNPRAWQRSTSFHTAVSPTSCQDCHGLA
                     NGGGSTAGTNNDLPAGLTFSDTKTTSSVAPANTYVQITHNDKNVAGKDCYTCHTQVGP
                     GGTKWKSGQFHSKVTNLTLNGTTGRCDNCHMNVKPTVVVSGMDHSSIGTQDCSACHTY
                     PGTGTQAVGNWLGVGGGSAHTASFISSNANTCNNCHTSGGSTTPPIGMPTTVINSGST
                     GRYSKTINYAHTFTSTSAGSAGTSTVTYTMTDCLGCHTGAATKAMANNGAGYLDTKAW
                     VGTGTFNHRIPSSVSFNGTTATVNASAPIQSCLPCHANEALSGTKHNLNCAAPKSPTD
                     CVQCHRTGGSWNDSKGGGPSCK"
     gene            complement(12431..13204)
                     /locus_tag="VIG33_00555"
     CDS             complement(12431..13204)
                     /locus_tag="VIG33_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8269350.1"
                     /translation="MQPLYYELSVLVKAIHFKNLSAAAIHIGLSQPQLSRIIARIEEE
                     FKIVVLDRSAKRKSSWTPIAFEMAEIFDHSTRRLGSELQGLSQNNAVAEIHIGTLEGM
                     SNFAMETSKICFEELGIHKISLNIYDLNELDSNFLSGNLDLIFTFKTPGRQKFRHLLE
                     IGSQVLDKVNTNKKFGVYSTFEFGRAQKKELESFEFLFISNSLAVRKSWLDNVGGVGY
                     VPSDTRKGRVKEHEPIFLIGSELLSPRLWEKLESILSRK"
     gene            complement(13223..14752)
                     /locus_tag="VIG33_00560"
     CDS             complement(13223..14752)
                     /locus_tag="VIG33_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEY8269351.1"
                     /translation="MSASPELEAFTIPERLRMSFEELGPTFVKLGQVLASRPDLIPEE
                     YTNEFEKLHDRVQSLPFEVVEGVLKEEFGDSLYQKFSFIDPVPLGAASIAQVHKARLA
                     TGEVVVVKVQRPGIIQTINDDLNVLYMLSELLMAYVPETRPFNPTGIVDEFFKTLELE
                     TNFVVEANNIRRFSENFAAEDDIKIPKVYLELTTERVLTLEALEGVPLSQEGALKQEG
                     IDLNALIRKGLKAYMKMVFADGLFHGDLHAGNFFVLPNNEIGLIDFGVVGRLNNKTQA
                     AVANMLLALAKEDYDRLAYEYVDLAPFTDKVNVDLFAKDLRELIAPYYGLTLKNVNLG
                     KLLMSSSGVAARHHLSLPTELMLFFKSIVAIEGLGRRIIPDFDFLQYSIEVANELVRH
                     QLDPNKFMNEISMVLRESKNFIHALPRQANFLLRKINSPAHAFKLNVPQVDDLKRSIE
                     VSFNLLFLGVIIAALILSASFIFIQPTENNISGMPTMSFIQYAMALVLGIVAFFNYIK
                     K"
     gene            complement(14769..>17090)
                     /gene="rnr"
                     /locus_tag="VIG33_00565"
     CDS             complement(14769..>17090)
                     /gene="rnr"
                     /locus_tag="VIG33_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEY8269352.1"
                     /translation="LAKNLLQGTIKRHPDGFGFFIPDSADHPDVYIPRQSMQGVMTSD
                     KVLIEVTPERGGERFRGSVIRVISRGFKQLVGSFHIINDRQAVIKDEGKGWGEDLKID
                     LSDSMGAKEGQLVTAQILTYPEDGRKFSGRVIEIIGDALDPMNDIKRVIATNNIPQEF
                     SPATLAEAKKFPAEPAPADFKNRKDLRPLNLITIDGATAKDFDDAVYVEVNKQGFLLY
                     VAIADVSHYVRIGSSIDKDAYERGTSVYFPNFVVPMLPEALSNGLCSLNPQVPRLCVV
                     AEIQFDFTGATLSSNFYEGVMESKARVTYGQAQEVIDGAGDPKFNHVKETLLHCADLA
                     KILMAKRFKEGSLDLEIPETELEIDASGVPIDIIKSERLFAHRLIEEMMLAANVAVAT
                     FLDSRGVPALYRIHEPPNEEAIRVLEKYLWNFGSKTKLGSDKLQKRLTKALEEFEGKP
                     QAQILNILTLRSMSQAKYNMNNVGHFGLGFEFYAHFTSPIRRYPDLIIHRLLKNQVMQ
                     NSPYRLLSEEDLLTAGTILSAAEQRSTKAERQVQSIKKARFMQKFVGQELDGIISSVA
                     KFGIFVLLREYDCDGLVRTDDLGNDRFEFDEENLQLRGQRTGKVYNIGDSIRIQVAAA
                     DPELGQVNFQLPGATEKLEANGPSLRPLFGKTDKNKQRKSEDRHRKPRSQKTEQPKNG
                     KQYLFNGKNLAVPSSARSRKLQFADRSKLRFNVEDVDAKEDSEPRQEIKSGPSKNRQS
                     TRKNTEKRSQTPNHSGGLRKTRVSSGRRKNKAR"
BASE COUNT         4514 a         3679 c         4141 g         4756 t
ORIGIN      
        1 cttatgacaa ttttcacagt ttttctcgac taacttcgga tacttgtaaa taggctgttt
       61 agtcacttta tcaaagtgac agtctttgca tttcacttct aaatgcgcac catcgatcac
      121 ataacgggtg tgagtgttgt gatcgaagtt aattgctggt ttccatactt tgtcggtatg
      181 gcaagctgag caattattta aagagccaaa gtgcggattt tttaccgtgc caaaacggtg
      241 gaagtcatca tggcaggtca gacattgagt tttaagcccc agccatttgt aattttttgt
      301 agaggtctct ttaagaacag ccgggcgctg tttatggcat tcagtacact tgagttcagc
      361 atgacgtccg ccgagtttat agccagtgat tttatgatta aagttttcaa tcttccaact
      421 gtcttccgtg tgacaggtgg tgcattttcc tccggtgaag cggtgggaaa gattcgcttt
      481 gtgagtttcc tcatggcagc ttaagcagag tgcggtttta agctgaggcc atctatagcg
      541 gaaaattttc ctttttttat cagccaaatg gcaatcatta catttgatct cactgtgttt
      601 tccgaccaat ttgtaattgg catcacgact gtgatcaaat ttaatattat ctttccaagt
      661 tttcaaagta tggcaggcat tacaatcttt tttagcaaac tcgtttttaa agaaatgcac
      721 atcatctttt ctatgacagc ttatacagct tgtggctttg cctatatagc gaatatctcc
      781 ggggcgtaca gacttaggtc ctaacttctc tttgtgacat tcttcacatt tgatttcggc
      841 gtgtttacct tcaaggaagt aaccagtgag cttaccatgt tcgaaattgt ctttgtcgac
      901 cacaatcgaa tcaaagtttc ttccttgatg gtcacgatga cattccatgc aagttttagt
      961 ggtcaatcca tgtaaaccgc gcttctgggt gacgaagggt ctgatcggtt tatggcattc
     1021 taaacatttt tcgttgggaa cgccctttcc cgcatcgtga cacttgaggc agtccttgtt
     1081 ttccaggtct tggtgacctt ttattaatgg accaggggcc agaagtttac tgagaattcc
     1141 ttctttttca gctgaggcca tactggtgat aaccgtgatc acggagataa taataaaaag
     1201 taaaagcgat gaagatggtt ttctcaaatg ggcccccgca gaggagcggc atcacggcgt
     1261 ggcgcagttt ccgcttcgcc gaaacggtag ccgagtttaa agaacgaagt aaggatggtc
     1321 acagactcat ctgtagctct ttgaatagac ccagtccaga aaagtgttct gctaatggaa
     1381 ttggaaactg aaaattctat ttcgagggga tgaagcaaag tgccgtcctc atgtttttca
     1441 gtggcatatc ctacgtcgag actgaactcc cattttctgg agaaataccc tgcaccagtt
     1501 ctgaaaaagg tgtctgtgga gacaaaatta ttccgattcc cgccttttgc gtagaggtcc
     1561 cagttaggag cgaagacgcg cgattgaaaa taactcaacg aaacttcagt cttatttttt
     1621 tgatcgtagg cacgatgccc tgagatcgta ccgagagtcc agcgcgagct gcgaggggag
     1681 ccaatggaga attttgcctg ggcctcttgg taggggctcg ggggaagagt ttcgcggatt
     1741 ccctgatggc gatagtattc aatactatcc attgcgagaa ttcctaattc ggaagaaaga
     1801 ttttcagtgt attcctgctg ccaaattaga ctgccatttt ggatataact gcgtgggaca
     1861 aagtcgaaat acatgagaga taaaattctt gaattttctt cccattgata attcatttca
     1921 tgaaatagaa aggagcgatc tgtttcgttg ccgtaacttt cagagacgac tccgttggtc
     1981 aaataaaagt tgcgagccca gcctccggtt ctggattgat aagtcagacc ggcgccggcg
     2041 atattcgctt gcgggttgaa agtgagatac gaatgacttt ccgcttgggg attcttaccg
     2101 ccgaaaaaag aagaccaaag gctaggcgtc caacggtact cagcaagaac tccatctgta
     2161 tagatggctc cgacatcctg cacgggaaaa cgccctagac ttaaagagag ctggccaccg
     2221 gggttgaccc aacgggtatt gatttgtcgt gcttgaaatt catttctgtc agtgagttgc
     2281 agacgctccc gatcgagctt atcgaagaaa tcatgtttat cgcgaaggtc tgatacaaac
     2341 tcccaatttc cttctcccat attggtgacc ttataaaaga gccgcgaaga aaaaaccatt
     2401 tcgtcattgg agtctgagcc aaatgcatcg gtggtgaact tttcggtggc agtgaatcct
     2461 ccgaaaacaa aacgaccata ggatttcgcg gcaagagctt tgttctcctt gcaaaataag
     2521 gatgcgatta ttaaaaaaat cgaaaagacc ttgatcaatt tcatttgacc aatatgatta
     2581 caattcagtg aacggttaat gacaaggaaa aacacatggg tcttgctaac gtagtttctc
     2641 gcctgaataa gaataaattt tttagattta tctcctctgt aaggctggca gtgccgacga
     2701 tgttaatcct gtgtgtgctg gtcgggtggg gaacaattct agagagcctt tataatgccg
     2761 aatacgcttc tctcagggtc tataagtctt ggtggttctc cattttcatg ttgattttat
     2821 ggctcaatat tttttgtgct ccattagctc gttatccatt caaaaaacac cagatcggat
     2881 ttgtggtgac tcatattgga cttttaattc tcctggctgg agggcttgta accaatcgtt
     2941 tgggaatcga cggacagctt caggtgatgg agggaacgag caactcctct gtgatgcttt
     3001 cacgcctgat ggtgggatat cagacggaag gaagtccaag tattcagaag atagtgtttg
     3061 atcgctctat aaactcaaaa gacagttcta atctggactt tatcaataaa gaattgggtg
     3121 catggtttaa ggtaaatcaa tatttgcctt ttgctaaagt ggaaaagaac tatgaatcta
     3181 gtccagtgac ttcagatgaa acgagcatca gctttatcat gaagagtcaa ttttttaacg
     3241 tgacggagtg gttgagcacc caggatgagc cggaaaagca aatgggtccg gcaactttgc
     3301 ggatcgtgaa aggcgagtac gttgccgcca ataacagtcg aaagaccgag aagaagacaa
     3361 ataagaagtc tgagaaaaaa gagagcttaa agaaaagaac cacttccgcg gtttcaggcg
     3421 ctgagatggt acaaatcctg gatgctcaga cagaaaaaga aattcaaaca gtgcctctgg
     3481 caaaactttt gaatacacct gtggtggtca atggtgtgac cattacagta agtaaaaaat
     3541 taaataatgc tatcgttgcc gccaacaaat tagctgaagg ggacccgagt ggttcgaacc
     3601 cggctttaga attcgaactg aaaaagggcg gtgaaacaaa gcgcgaagtt tcctacgtca
     3661 agcatgcgac tttctcattg catccacaag gagcctttgg cctgaggctt caatatcttg
     3721 gcggaagtgg cggaagtgca atcggcccgg caatgagtga agaaaacatt caggagaaca
     3781 gcgagcctat ggcgatgcaa cgccctggcg gcaatgtgat cgagtttcac atcccaagcg
     3841 gaaatcagaa caacaatcaa aaagttcagg tggttctgtt taagaacggc gaatttgttc
     3901 aagaagcttt tgtcggcccc ggtgagagtg tgcaaacacc gtggatggga atgcagctat
     3961 ttattggctc cgtcattcac aatagtcagt ccgcagtggt ggcaagaccg gtggcgcttg
     4021 aaaagaaaag caatcttcca ccaagtgcgc ttgaacttaa tgtcgacgct gcctctcctt
     4081 ctttgtggct ttctgagggc gatgaaaaag aaattatggt gggtaacaaa agaattcatg
     4141 tcttttacgg acgtgaaaat ttgaccctgc cctttgagat tgctttgaaa aagttttcta
     4201 agattgacta tcccgggact gaaactccga tgtcttatga aagtttggtt caaatcaatg
     4261 gccatggacc tgttacaaaa atttccatga atgaacctct caaagttgaa ggatacacga
     4321 tttatcaagc cagttattct ttacagccga atggcccccc ggtttccgtg ttctcggtga
     4381 ataaagatcc agggcgtttt tggaaatatt taggaagtat cattcttgcg atcggtgtga
     4441 tcctttatac gctgatgaaa agtcgtctgt atcgtaaaga aaaaagagga gtttaatccg
     4501 atgaagaatc taatgaaatt gacgctagta ttagcgttag aaatattttt caccgccgct
     4561 gtttatgctc aacacgaagg aatgaatcca aaagtcgaaa gtgcaacccc agaaaaagcc
     4621 atgatggatc ttaaaatcca aggtgagcaa attcagaaag atcccattcg ctctttaatg
     4681 gtccatcaga atggccgcct gaagcccttc gatacgtttg cgcaagaaag tattttattt
     4741 ttaaatggct cttataaaag aactggtctt catccagttc aaacttattt agccctcatg
     4801 acttcttcga cggcgcctta tgtggaactg gtcgaagttc gtgatccgga acttcgcgtg
     4861 caaatgggct tcatgaaatc caaacgtttt atgtccttgg cggatcttga aagttccaat
     4921 atttcaagtt tgacggagcc actctttaaa aaacaagaag agaattccaa gtctttgacg
     4981 tccgcagaga aaaccgtgct tgagactttt aatcaggcca tgttactgca agcggtgatc
     5041 cggggtgatc acttggcgcg ggccgcggac tttagtttct taaaaggaac gcatggcgaa
     5101 gagatggctg cctctgaagt acaagcggct ctgcgagaat atatacaatg tttaatgagt
     5161 tctgaatgca gtcaaggaat caaggctgaa gctttactcg cagtctccgg caagcaagag
     5221 actccggata tgtttaaaca ttactttgag aagaccaaag cggaaatttt ctacaatgat
     5281 ttccgtcctt ttttctgggc cagtctattt tatatcctcc taggggtttt attctccctc
     5341 agctttatcc gtaataaaat tggcaccaaa tggctttcgc tgctactcgt tcttccttta
     5401 tctttgcata ttctgggttt aggtctgcgg gtttatatca ctcagttcgc gcctgtgacg
     5461 aatatgtacg gaaccatgat ttgggtctct cttggggtga cggtctttag tacactttta
     5521 tttattcttt acaaaaatgc ctatgtcctt tgtgcggcgc ttatttgcag tggcttgatt
     5581 ttgatgctga ctgagcaaat ccccatgatc ttgagtccgg atttagatcc gatcgtggca
     5641 gttctgcgca gcaacttctg gctttccact catgtcacga ccatcacgat ttcttatgcg
     5701 gctttcacgg tggcggcggt tttgggaaat atcgctttgt ttcgagtatg gattcataaa
     5761 gagaacgacg ccttttttaa agagtactct cattatgctt accgcatgat tcagctgggg
     5821 tgtttcttat taacagtggg aattattctt ggcggtgtgt gggcggatta ttcctggggc
     5881 cgtttctggg gttgggatcc gaaagagact tgggccttga ttgcggatgt gggcttttta
     5941 gcaatactcc atgctcggat cgcaggttgg ctcaagcctt tcaccttact tgcattttcg
     6001 cccgtggcct atttgttggt gattatggca tggtacggag tcaattttat cttggcagca
     6061 ggtttacact cttacggatt ttccagcggc ggtgccctgg tcgtcgccat ttatgttgtg
     6121 gtgcaggcag tggttcttgc gctgggcttt tggagatact ttagtttgaa aaaggtggcg
     6181 gcttgatagg gaacattctc ggagatttag atccgagaat aggacagatt tacctgtatg
     6241 ttctattcct tctcgggcta gcagtgacca tggatttgca taaattaaaa acggccccag
     6301 agggaccgct tttaaggaag agagactccc cctaaggagg cactctatga aaagaaaaga
     6361 gacagtttct tttcggatat ctagggaatt actaaagttg ctgatgctta tttttttgtt
     6421 gtttttcagt tgctgtagtt aacctgaaga taaagtgaga ctaacttaaa gggctttcga
     6481 atttttaaac tatggtctag gtcaaagtgg gcttgatcgg gcacaacccg ggagctttgc
     6541 atggacgatt ctctggggcc acctttattt gctgttcgag ggaaagtaaa attcaaaaaa
     6601 gctctgcaaa atttcacacc ccaaacatga gtgccgctgt gacatgaata atcgcagcga
     6661 catacatgaa gatcgcaaat ggaaagtgaa aggcatgcca gtaacccatc aaccgttgaa
     6721 agggtgccag caaggctgaa cgtcgtttgt taacggcata gtcttttaaa ataaatttgg
     6781 tggcggttgg ccactgcgga ccaatttctg gttcggtgaa tgccattcgg atgtctccgg
     6841 ccatagaata aaccaatgct ttaagaggat tgtaaggagt atcagttaaa ggggctccgg
     6901 ccaattgcag agctttttta gagtaaagct cttgatcttg ctgggtgaaa ggaacattaa
     6961 ttcttgccag catgcgaccg atcttggcca aacgctgctc ggcctctttt tcgaattctt
     7021 ttttagcttg taacatctgt atgtagaaat aacgtccaat gagacctgaa ctaaaagaaa
     7081 taaccatgga ccaaaaactg atgcccacga cgccgcggac tttaaaattg caatgaaaga
     7141 gaatgaaagt cggaccgatg aggccgcata aaatatgaaa attcaaccaa gacgtgagct
     7201 taccgctgtg agatagccac gaaagtcgtt ttcttaaaga gtaaacattc atcatgatca
     7261 taagccctaa gcctgtccat cctagaaaga gtgacagttg gcgccccggc tctttgttga
     7321 tttggtagcg cgcgggaaga tagcccatgg cctgtaattt tttaagtagg gaaagcgact
     7381 cattaaagtt gaggccgtag tactcgtagc ttaataaagc taggaataga atccaaataa
     7441 acatgacgct tttaaatcgc atttttcata ccttcgccaa attttttatc gatctccacg
     7501 cccaggccca ttaaaaattt atgggggagt tctgccccgg caaaaataaa aacaaagtca
     7561 ttgcgaagat cgatgatctc ctcttgtttt tttatctttg tcagacctaa ggcaatggct
     7621 tgaacggaag agttgtacca gatactcaac aaacctttgg attccatttc cagaatgcgt
     7681 ttcatattgt cctcgttaca gcggtcaaag gttgggccgc ggacgagcaa gtgaacttca
     7741 tttctccagc gtttttcact gagcatttgc gcggcttcca ccgctgcatt accgccccca
     7801 acgacaatga ctttcttccc ttggtattgt tcagggtcga gaaggttata ggtcacttta
     7861 ggcaggtctt ccccaggaac tcccaggcgg cgcggactgc cgcgaacacc catgcataaa
     7921 atgacttttc gtgcggtgta ttcacctttg gaggatttta ctttaaaaat atttccagtt
     7981 ttttcgaggc cttcgaattt ggtgtcaact tgaactttca ggccagtttt tttacgcaca
     8041 ttattccaat attttagcag ctgttcttta gaaatccgat tgctttcaaa tttgaatttt
     8101 ccaatgagag gcaactcgaa aggatgactc atgactattt tctggcgtgg aaaattataa
     8161 accgtgcctc caaaagagtt ttgttcaaga agtaaatagg attttttttc agcgatggcc
     8221 gttacgccgg aagcaaggcc tgcggggcca ccaccgacaa tgatcacgtc atattcggcg
     8281 ttctcttgtg ggagtttttt gacggcatca gtggttgcca gaattccttg tttaatcgca
     8341 ttgcgaataa gtcccatacc gccgagttcg ccagcgatat agagaccggg cacattggtt
     8401 tcataattgg aatttaagcg aggaatatcc atgccccggg ttttagttcc aaaaacaagt
     8461 ttgatcgcat tcaaaggaca agccttttcg cactctccat ggccgacaca tttcgtgggc
     8521 cctacgagga cagctttatg attaatcagt tttataatgt ccccctcagg gcatatcgtc
     8581 gtgcaggctc cgcagccggc gcataagcca gaatcaattt caggatggag agacatgggc
     8641 tcattgaggc cgcttttgac ggctttcgat aataaatttt tggcttctcg gtgatgcttc
     8701 tcacgacgaa ggattgaaag cattgaataa aaacccacga gaacaataaa cgtgaggaca
     8761 acattgtccg cgatgaaatt taaaaaaccc ataggattct cccatatttc taattatcgg
     8821 aaaataacaa agaaaaccca atacctgaga gaagagttag actttcggag agggagctat
     8881 ttgcagctcg ggcctccgcc cttggaatcg ttccatgatc caccagtacg atggcattga
     8941 acgcagtctg taggagactt tggagccgca cagttaaggt tgtgtttagt gcctgataag
     9001 gcctcattcg catgacatgg caaacaagat tgaataggcg ccgaagcgtt cactgtagcc
     9061 gtggttccat taaaggaaac agagcttggg attcgatgat tgaaggttcc agtacctacc
     9121 catgctttgg tatctaaata gccagcacca ttattggcca tggcttttgt cgcggcgccg
     9181 gtatgacagc ccaagcaatc agtcatcgta taggtgacag tggaggtgcc agcactacct
     9241 gcggaagtgc tggtaaaggt atgggcatag ttgatggttt tcgagtaacg tcctgtagaa
     9301 ccactattga tcacggttgt tggcattcca atcggtggag tggtcgagcc gccgctcgtg
     9361 tgacaattat tacaagtatt ggcattggag ctgataaagc tagcggtgtg agcgctgccg
     9421 ccacccactc ccagccagtt accgaccgct tgagtgccag tccctggata ggtatgacag
     9481 gcactgcaat cttgagtgcc aatagaacta tggtccatac cactcaccac aactgtcggc
     9541 ttaacattca tatgacagtt gtcgcagcgg ccagtggtgc cattcaaggt taagtttgta
     9601 actttagagt ggaattggcc ggatttccat ttggttcctc ccggtccgac ttgggtatgg
     9661 caagtatagc aatccttacc agccacattt ttatcattgt gggtaatttg cacgtaagta
     9721 ttcgccggtg cgacactgga agttgttttt gtatctgaga aagtcaatcc tgcaggaaga
     9781 tcattgttag ttcccgccgt cgaacctccg ccgttggcga gaccatggca gtcctgacag
     9841 ctcgtcgggc tgaccgctgt atggaaggaa gtgcttctct gccaagcgcg tggattggtg
     9901 ttcagatcat tcagatggca agtcgcgcat tcagtaccga ggtttgtgga aatatgactc
     9961 atgtgttgcg aatcagtagt actgaccgtg actcctgagg gcttttggcc cgcgtggcag
    10021 tcaatacatg cagtaggtgg cgtggtgatt tttccgtgat agaggccgcc ggcccaattt
    10081 gttgaggcgg ggttcgtggc ttgcgctgat gtgggctgtg catggcaggc taaacagtct
    10141 tgaacagcga aattagatgt gtgggccatc ttattaatca gggtttggtt catgatcggg
    10201 tagtggcatg tggtacagtt ggtcggagac tttgaagaaa ttttaaaatg gaatttccca
    10261 tcagcgaagg tgactcctgg ttgagcgtgg caagtcgcac agtcaaaatt gctaacagcc
    10321 gtgcctcctc cagataatgc cgccgcatgg ttcatgggct tcaaagcggt gccgactata
    10381 tcgaccggca gtgtgcctgt atggcaatcg cggcaacttg taggctgtgc aagacccttc
    10441 accgttaaag acgcatgaaa ctttcctccg gcgtaaacac cgctactcgc ttggttgtgg
    10501 caagtcgcgc agtcattggc aatgctggca gggacttgag tggaggtatg cagcatttgt
    10561 tttggtaatg attgggtttg ctgcgaagtc gaaatcaccc gagtgccaga ataatttaaa
    10621 tgtgttgagg tgacacttaa tgagccagct ccgacgtacc ctgaagggaa agtagtggca
    10681 cttccccagt tagccagact ggtaaattga tttttagtca atacttgttg atggcatcct
    10741 atacaatcac cggttccact tttggagtga tcaaagccat taataatacc agtcggacgt
    10801 tgcgagctat gacatgagga acaagaggta aggctggagg agtgtacaaa atttccccca
    10861 ctccattgag attttgcggt gaattgagtg gtcgcagtgt gacaagtggc gcagtccagg
    10921 gggctcccgt gagtgttaaa atcaaaaggc gtattgggat tcgaggcggt aaaacttgag
    10981 ttggtattag gccgtgaaga ctcgtggcaa gcgatgcacg aagcaggttt gccattcgcc
    11041 gcatggaaaa ctccgccacc ccaagtgctt tggcttgtat gacaagtgag gcaatctccg
    11101 gtgccgccca aattgacgtg atcaaacttg gtggttcccg cagccgccgt cacaggtaca
    11161 gggccgacag gacggctgtt ggcatggcaa tccacgcaag aacttggttg agttaatcct
    11221 gcaccgacga gtgactgatg gaattgggct gactgccaag ccgtgcctgg ttgtttatga
    11281 caggtcgagc agtcactatt tacgatggga gtcgtggttg cgacataatt accagcggca
    11341 ttttttgccc aaacgaccgc atcgtgtttc atgactttgg aaggaggaat tcttgatgaa
    11401 tccgaatgtc ccacgaagcc tttgggtcgg accgcgccgc tcgtatggca gtcactacaa
    11461 gaggttggct gtcccaggct ggctttggtg agcgaaggat gaaactcccc tggtaaataa
    11521 tttccacttg caatccctgc atggcaaaga gcgcaatttt gcaaatcagt ggcttttagc
    11581 tgcgttgttt tgtggttcat tgccatatga taggattgat tttgtgatgt gagagattga
    11641 atggaggtat tcaagtaagt gggaatctgc gcgacgatat tgatatccgc tgaaggatcc
    11701 caaaccagcg tcgcgggagt tccgccagct ccgctccaat cgctaagact actgaaactg
    11761 ctctgcaaag cgggttgatg acaagaaaag cactctcctg tgccgctcgt gctgtgattg
    11821 aaaccattga caatggtggt gggtcgttgg ctgctatgac agcttacaca agaagttaag
    11881 aggggggaat ggacatactt gccgccagcc caatctgccg aagtgcggga aagtgtttga
    11941 ggatgatgac aagtaataca atcagttcca gcagcatggg aaaaataatc gaagggcgta
    12001 tttggattga ggccgataaa gcccacagtg gtattcggtc tagagagttc atgacaagtg
    12061 gaacaagaag tctcttggcc ggtatgtttg aaggacacgc ttgcgtattc cgcattttcc
    12121 acactaggcc tctccacacc acaattttga aacatcaaaa tcgccgtgcc gagtgcggca
    12181 aagggtaata aaattttaag ctgtctcatt gtaatacgca tttagttttg aatccccaaa
    12241 tcagtctacc tgtcggttga attaatttcc aacattatgt cctgggtgtt tctattttag
    12301 aattcgaatt gttgcgctgg cgatcgacgg gctggagctc gtgcacaagt actgttttga
    12361 tatgcattcg aatgtctgga tctcttatgt caaaaagatg gacgaattct tcaatcgaac
    12421 cgaaatagaa ttatttccgc gacagaatgg attcgagctt ttcccacagc cgggggctta
    12481 gcaattcgga gccaattaaa aagataggct catgttcttt cacccggccc ttgcgcgtat
    12541 ctgagggcac ataaccaact cctcccacgt tatcgagcca agatttacgg acggctaaag
    12601 aattagagat gaataaaaat tcgaaacttt ccaattcttt cttttgagcg cgaccaaatt
    12661 caaaagtgga atacactcca aactttttat tggtgttcac tttatcgaga acttgagatc
    12721 ctatttccaa tagatgtctg aacttttgac ggccgggagt tttgaaagtg aagatcagat
    12781 ccagattgcc cgataaaaaa ttcgaatcca gctcgttcag gtcgtagata ttgaggctga
    12841 ttttatgaat accgagctct tcaaaacaaa ttttggaagt ctccatggca aaattggaca
    12901 taccttcaag ggttccaata tgaatttcgg ccacggcatt gttttggctg agtccttgga
    12961 gttcacttcc cagacgacga gtcgagtggt cgaagatttc agccatttcg aaagcaatcg
    13021 gcgtccaact ggattttctc ttggcggaac gatcgagtac aacgatttta aactcttctt
    13081 caatacgggc aatgatgcgt gaaagttgag gttgactgag ccctatatga atggcggcgg
    13141 cagagagatt cttaaagtga atggccttca ccaaaacact gagttcataa taaagtggtt
    13201 gcatgacgct tccttaagaa aattattttt tgatgtagtt aaaaaaggcg acaataccca
    13261 aaaccaaggc catggcgtat tgtatgaaac tcatggtggg catgccgcta atattatttt
    13321 ccgtgggctg aataaagatg aaggaagcgc tcaaaatcaa agccgcgata ataacgccta
    13381 agaacaacaa attaaaggag acttcaatag atcttttcag atcatcgacc tggggaacat
    13441 tcagtttaaa ggcgtgggcg ggactattga tttttctcag aagaaaatta gcttggcggg
    13501 gaagggcgtg gataaagttt tttgactccc gcagaaccat cgatatttca ttcataaatt
    13561 tattggggtc caattgatgt ctgaccaact cattggccac ttcaatggag tattgcaaga
    13621 aatcgaaatc aggaatgatc cgccgcccaa ggccttcaat ggcaacgatc gacttaaaga
    13681 ataacatcaa ttcggtgggc agacttaaat gatggcgagc cgcaactcca ctggaagaca
    13741 taagaagctt gcccagattt acatttttaa gagtgagacc gtagtaagga gcaatgagtt
    13801 cccgtaagtc cttggcaaaa aggtctacat tgaccttgtc agtgaaggga gctaagtcca
    13861 catactcata agccaggcga tcatagtctt ctttggctaa tgccaaaagc atattggcca
    13921 cggccgcctg agttttatta ttcaagcggc ccacaacccc aaaatctatc aagccaatct
    13981 cattgttggg aagaacaaag aagtttccgg catggagatc tccatgaaag agtccatccg
    14041 cgaataccat tttcatatag gccttgaggc ctttgcgaat caaagcgtta aggtcaatcc
    14101 cttcttgttt cagggcgcct tcttgactta aaggaacccc ttccagggct tccaaagtca
    14161 aaactcgctc ggtggtgagt tccaagtaga ctttcggaat tttaatatcg tcttccgctg
    14221 caaagttctc tgaaaaacgg cgaatgttat tcgcctcgac cacgaaattg gtttccaatt
    14281 ccagagtttt aaagaattca tccacgattc cggtgggatt gaaaggccga gtttccggca
    14341 cataggccat caaaagttct gaaagcatat acaagacatt taaatcatca ttgatggttt
    14401 gaataatgcc cggacgctgc accttaacca ccacgacttc accggtggcc agtcgcgctt
    14461 tatgaacttg cgcgatactg gccgccccca aagggacggg atcaatgaaa gaaaattttt
    14521 gataaaggct gtccccaaat tcttctttaa gaacgccctc caccacttca aagggaagac
    14581 tttgaacgcg atcatgaagc ttttcaaatt cgttggtgta ttcctcaggg ataaggtcag
    14641 ggcgagaggc cagaacttgc cccaacttta caaaggtggg ccctaattct tcgaaactca
    14701 tacgcaggcg ttcgggaatc gtgaatgcct caagctcggg tgaagcgctc atgcgctcca
    14761 caatgaattt accgagcttt attttttcgg cgacctgatg aaacccgtgt cttgcgaaga
    14821 cccccactat ggttcggagt ctggctgcgt ttttcagtgt ttttcctagt tgactgcctg
    14881 tttttagaag ggccactctt aatctcctga cgaggttctg agtcttcttt tgcgtcgacg
    14941 tcctcaacat taaatcgcag ttttgaacga tccgcaaact gcaattttcg agagcgggcg
    15001 gacgagggaa ctgcaaggtt tttcccatta aataaatatt gcttgccgtt cttaggctgt
    15061 tccgtcttct ggcttctggg cttgcgatga cgatcttctg atttccgctg tttattttta
    15121 tctgttttgc caaagagagg gcgcaggcta gggccgttcg cttcgagttt ttctgtggcc
    15181 cccggaagtt gaaagttcac ttggcccaac tccggatctg cggcagcgac ctggatacgg
    15241 atactgtcac caatattata aactttgcct gttcgttgtc ctctgagctg caggttctct
    15301 tcatcaaatt cgaatcgatc attgccgaga tcatcagtcc gcaccagacc gtcacagtca
    15361 tactcgcgca gcagaacaaa gattccgaat ttcgccacag aactgatgat accatcgagt
    15421 tcctggccga caaatttctg catgaagcgg gcttttttta tcgattggac ttggcgctca
    15481 gctttggtgg acctttgctc cgctgcggaa agtatcgtcc ccgcggttag taagtcctct
    15541 tcgctcagca agcggtaagg agagttttgc atcacttgat ttttcaacaa gcggtggatg
    15601 atcagatccg gataacgacg aatgggcgaa gtgaagtgag catagaactc aaatcccaag
    15661 ccaaagtgac cgacgttatt catattgtat ttcgcttggc tcatggagcg aagggtaaga
    15721 atattgagga tctgagcttg tggttttcct tcgaactctt ccagagcttt ggtcagacgt
    15781 ttttgcaact tatctgaacc gagctttgtt ttgctgccaa agttccacag atatttttct
    15841 aaaacccgaa tggcttcttc gttcggaggt tcgtgaatgc gataaagtgc agggactcct
    15901 cggctgtcca gaaaagtggc cacagcgaca ttggcagcca acatcatttc ttcaatgagg
    15961 cgatgggcaa aaagacgctc tgatttaata atatcaatgg gtacgccgct cgcatcgatt
    16021 tccagttctg tctctggaat ttcgagatcc aaggagcctt ctttgaaacg ttttgccatc
    16081 aggatcttgg ccagatccgc gcagtgaaga agggtctctt tgacgtgatt gaacttaggg
    16141 tcgccagcgc catcaataac ttcttgcgcc tgtccatagg tcacgcgggc tttgctttcc
    16201 atcacgcctt cataaaaatt ggacgaaagc gtcgctccgg tgaagtcaaa ttggatttcg
    16261 gccaccacgc aaagtcttgg aacctgagga ttgagcgagc aaagaccatt gctgagagct
    16321 tcaggcaaca tcggaaccac aaaattcgga aagtaaacgg aagttccgcg ttcataggca
    16381 tctttatcaa tagagctacc gatgcgaaca taatggctga cgtctgcaat ggcgacataa
    16441 agcaagaaac cttgtttgtt cacttcgaca taaacggcat catcaaaatc ttttgccgtg
    16501 gcaccgtcaa tcgtaatgag attgagcggg cgcaggtctt tacgattttt aaaatcagcg
    16561 ggggcgggct ctgccggaaa ttttttagct tcagcgaggg tagcgggcga gaactcttga
    16621 ggaatattgt ttgtggcgat gactcgttta atgtcattca tcggatccag ggcatctccg
    16681 atgatttcaa tgactcgtcc agaaaatttt ctgccatctt caggataagt aagaatttgc
    16741 gcggtaacga gttgcccttc tttagctccc atggagtcag agaggtcgat cttgagatct
    16801 tcgccccaac ctttgccttc gtctttaatg acggcttggc ggtcattgat aatatggaaa
    16861 gaacccacga gttgtttaaa gccacggctg ataacgcgaa tgacactgcc gcggaatctt
    16921 tctccgccac gctctggagt gacctcgatc aaaactttat cactggtcat aaccccttgc
    16981 atggattgcc gaggtatata gacgtcggga tggtccgcgc tatctgggat gaaaaacccg
    17041 aatccatcgg ggtgtctttt gattgtgcct tgaagtaagt ttttggctaa
//
LOCUS       DATBTE010000019         5669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2504, whole genome shotgun sequence.
ACCESSION   DATBTE010000019 DATBTE010000000
VERSION     DATBTE010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2504"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VIG33_00570"
     CDS             <1..222
                     /locus_tag="VIG33_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269353.1"
                     /translation="SKRAFCKIHGLSMSTFQYWQRKLNQAKKREVEVLKPSPFIKVDV
                     EPQALPASRNRPDARWVAELILHLQEGLR"
     gene            219..575
                     /gene="tnpB"
                     /locus_tag="VIG33_00575"
     CDS             219..575
                     /gene="tnpB"
                     /locus_tag="VIG33_00575"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEY8269354.1"
                     /translation="MKSPKDFKGVFLHRDPVDMRKAINGLSVLVETSGMGDLMGPYLF
                     VFCGKRRDVMKVLYFDKSGFALWVKRLERDKFPWPKKSTLENVSLSTEQFTWLLEGYD
                     IWKVKPFTELNFENVC"
     gene            650..2158
                     /locus_tag="VIG33_00580"
     CDS             650..2158
                     /locus_tag="VIG33_00580"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEY8269355.1"
                     /translation="MALRIAELEHLLKKEQEKNKNYESQVGWLTEQLKTLKRNQFGSK
                     SERWESQEQLKFNEAEVEAKSPVPEKEDYTVEVRGHTKKVRGHRKPLPENLAREVVKI
                     ELPEAEQVTEQGEKLKVIGWEISEKLKYEPAQISVIEYHRAKYGVDSGDYEKTAPPVP
                     SVIPKGIATPELLAAIVVGKYSDGLPLYRLEEIFARQDVELSRTTMARWMVQVAAALI
                     PIRNVLSDRMVSAHAISCDETSVQVLKENGRPAESKSWMIVRATPCEKSKIVLFDYSQ
                     SRSGEVMKNLFAEYQGRLLCDGLDVYNGLESETLLRFGCHMHARRKFEQAATDGAPSG
                     KTIASGVMEIYKKIYKFEEKIREHAPEDKIKARLQDQKPLLEEIKQIAESSINKVPNK
                     CKLSQALRYYLNEYTYLTRYLEDGLMEPDNGFTERAIRKFAIGRNNWLFSDTPEGAEA
                     SSLLYSLVVTAKMNKVNAYKALSKILTEIPLAKTYEDYERLADSILTPEPSA"
     gene            2171..3121
                     /locus_tag="VIG33_00585"
     CDS             2171..3121
                     /locus_tag="VIG33_00585"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEY8269356.1"
                     /translation="MGRLPFNRWKYDIFDKRNGPLSVPANKLTEDEVAEIIKISTSKK
                     YMDLSPCQIVPSLADSGFYVASESSFYKTLKEHNLLSHRGKAKPRNNNRPAPLVATGP
                     NQIFSWDITYLRSSVTGMFYYLYMFMDIYSRKIMGFKVHECESMNYSANLISEICLKE
                     KIQKHKLILHSDNGGAMKGATMLATLQKLGVVPSFSRPRVSDDNPYSEALFKTLKYCP
                     EYPAKPFSSIKDAADWVEKFVYWYNNDHLHSGIKYVTPALKHDGRDIEILENRKKVYR
                     EAKIKNPNRWSKEIRNWNRVEKVYLNYLQEENDVVINCAS"
     gene            3180..3602
                     /locus_tag="VIG33_00590"
     CDS             3180..3602
                     /locus_tag="VIG33_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269357.1"
                     /translation="MTETFEALSIEKAAVAERLSRLDEQSDAREFVRESIEGVSKNLN
                     DFKRGFVKAKGSLKKRLLRRLLKQVVLTSEGLLVFMYLADGSDIPNHQIKLVKELDKK
                     TNEIEAIYLTRKASGDDSNLSVLRSPIGRIPSSRGALL"
     gene            complement(3661..5025)
                     /gene="ltrA"
                     /locus_tag="VIG33_00595"
     CDS             complement(3661..5025)
                     /gene="ltrA"
                     /locus_tag="VIG33_00595"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEY8269358.1"
                     /translation="MGSYSKSTPREQKKTSLAPHTLTLQNKIRNQTNQNRKQWDLYRH
                     LMDPYLLAEALSLVIKNDGTGGIDRVTIDRIKGHEWGFVKELAAEMKSKSYKPLPVKR
                     VFIPKSDQSLRPLGIPALRDRVIQRALVLILEPIYELRFLDLSYGFRPAKSARDCAAK
                     VADLCYHHRHVIDADIEAFFDRVEHRKLMGMLKHEITDPRLLKLIQQILKAGFIEPGK
                     PWQPTPEGTPQGGPLSPLLANIYLHYSLDKKFAEWNLKDAHLVRYADDFVVLAKIKQS
                     AEFLLRMIKAELKRVGLNLKESKTRLVNMKNRFRGHESHFNFLGFKFHLRAFEDCPKR
                     FWVARQPSEKSRKNLSRKLKESLMPNLSLAEAKKKAKEIWDGWSEYFRYSNANRIFYR
                     QVNVVRQQLARYLRRKFRRQRRPVPWNILYRILRTLTQGIRPKRVINDLVRQKKNSNL
                     AFDF"
BASE COUNT         1577 a         1187 c         1352 g         1553 t
ORIGIN      
        1 tcaaagaggg cattttgcaa aatccacggg ctttcaatga gcacgtttca atattggcaa
       61 cgcaaactta atcaggcaaa aaaacgtgag gtcgaggttc tcaagccttc cccctttatc
      121 aaagtggacg tcgagccaca agcgcttcct gcaagtcgca ataggcccga tgcaagatgg
      181 gttgctgagt tgattcttca tcttcaagag ggcttacgat gaaatcccca aaagatttta
      241 agggggtgtt ccttcacaga gatccggtgg acatgagaaa ggcgatcaac ggtttaagtg
      301 ttttagttga aaccagcggc atgggggacc ttatgggccc ctatttattc gtattctgcg
      361 gaaagcgacg agatgtgatg aaggttcttt attttgataa atccggtttt gccctgtggg
      421 ttaagcgact ggaaagagat aaattcccgt ggccaaagaa atccactttg gaaaacgtga
      481 gcttatccac agagcaattt acttggctgc ttgagggata tgatatttgg aaggtgaaac
      541 cctttactga attaaatttt gaaaatgttt gttgagagtt tttttaaatt ccttaatact
      601 cgctgacgtc atgtcaaagt cgaactcact tttatcttca gaacaagaga tggctctacg
      661 aatcgcagag cttgagcatc ttttaaagaa agagcaggag aagaataaaa actacgaaag
      721 ccaggtgggt tggctgaccg agcagttaaa aaccctgaag cgcaatcaat ttggaagcaa
      781 atccgagcgc tgggaatccc aagaacagtt gaagtttaat gaggccgagg ttgaagcaaa
      841 aagccctgtt cctgagaagg aggactacac tgttgaggtg cgaggccata ccaagaaagt
      901 tcgggggcac agaaagcctt tgcctgagaa tctggctcgc gaggttgtaa agattgaatt
      961 gccggaagct gagcaagtca cagagcaggg agaaaagctg aaggtcattg gctgggaaat
     1021 ctctgagaaa ttaaaatatg aaccggccca gatcagcgtc attgagtacc accgcgctaa
     1081 gtatggagtg gatagcggag attatgagaa gaccgcgccc cctgtccctt cagtgattcc
     1141 aaagggcatt gccacaccag aactcctggc cgctattgtt gtgggtaagt atagtgatgg
     1201 tcttcctttg taccggttgg aggagatctt tgcccgtcaa gatgtggaat taagtcgcac
     1261 caccatggcc agatggatgg tgcaggtagc cgcagcctta atccctatcc gcaatgtttt
     1321 atcagatcga atggtttctg cgcacgctat ttcctgtgat gaaaccagtg ttcaggtttt
     1381 aaaagaaaac ggtcgccctg ccgagagtaa gtcgtggatg attgtgcggg ccactccttg
     1441 tgagaaaagc aagatcgtat tgtttgatta cagccagtct cgcagtggcg aagtgatgaa
     1501 gaatctattt gcggaatacc agggacgtct tttatgcgat ggtcttgatg tttataatgg
     1561 gcttgaaagt gaaacacttc ttcgctttgg ctgtcacatg catgctcgcc gcaagtttga
     1621 gcaagccgca acggatgggg ccccctctgg aaaaactatt gcttcaggag tgatggaaat
     1681 ctacaaaaag atttataagt tcgaagaaaa aatcagggaa cacgcccctg aagataaaat
     1741 caaagcaagg ttacaagatc aaaagccgct cctggaagaa ataaaacaaa ttgcagaatc
     1801 cagtatcaat aaagttccaa ataagtgcaa gttgagtcag gcccttaggt actacttgaa
     1861 tgaatacacg tatctgacaa gatacttgga ggatggattg atggagcccg acaacgggtt
     1921 cacagaacga gccattcgga agtttgccat cggtagaaat aactggctct ttagtgatac
     1981 gccagagggg gcggaagcca gcagtcttct ctacagtctt gtggtcacgg caaagatgaa
     2041 taaggtcaat gcttataagg ctctctctaa aatcctcact gagatacctt tggctaaaac
     2101 ttatgaggac tatgagcggc tggccgactc gatcttaaca ccagaaccat cggcttaaca
     2161 aaaaggcttc atggggcgct taccttttaa cagatggaaa tatgatattt tcgataaaag
     2221 aaatggacca ttaagtgttc cggccaacaa gttaacagaa gatgaagttg cagaaattat
     2281 caaaatatcc acatcaaaaa aatatatgga tctttctccg tgtcaaatcg ttccctccct
     2341 tgcagatagc ggattttacg ttgctagcga atcatctttt tataaaactt taaaggagca
     2401 taatttatta agccacaggg ggaaagcaaa accgaggaat aataatcgac cagcaccttt
     2461 ggttgctact ggcccgaatc agattttctc ttgggacata acctacctca gaagttctgt
     2521 cacaggcatg ttctattacc tttacatgtt catggatatt tacagtcgga aaattatggg
     2581 atttaaagtt catgagtgcg aatcaatgaa ttactccgca aatctgattt cagaaatttg
     2641 tttgaaagaa aaaattcaga aacataagtt gatacttcat tccgataatg gtggtgccat
     2701 gaaaggagct acaatgcttg ccacacttca aaagcttggt gttgttccct catttagccg
     2761 acctagggta tcagatgata atccgtattc ggaggcgtta tttaaaacgt taaagtattg
     2821 tcctgagtat ccagctaaac ctttttcatc aataaaagat gccgctgact gggttgaaaa
     2881 atttgtgtat tggtacaaca atgatcattt acatagcgga ataaaatatg taacgccagc
     2941 tttaaaacac gatggaagag atattgagat tttagaaaat agaaaaaaag tttatcgtga
     3001 agctaaaata aaaaatccaa atcgctggtc aaaagaaatt agaaattgga acagagttga
     3061 aaaagtatat ttaaattact tgcaggagga gaatgatgtt gttataaatt gcgcatctta
     3121 gtgatcaaga tcattggtca acaatccaaa aaaattccgg ttccgatagt ctaaaatgga
     3181 tgaccgaaac attcgaggca ttatcaatag aaaaggctgc tgtagctgaa aggttgagcc
     3241 gcttggatga acagtctgat gcccgtgagt ttgttaggga gtcgattgaa ggcgtatcca
     3301 agaatttaaa tgacttcaaa cgaggttttg taaaagccaa gggttcttta aaaaagagac
     3361 tcttgagacg actgctaaag caggttgttc taacctctga aggattgctt gtttttatgt
     3421 atttggctga tggttcggac attccgaatc accaaatcaa attggttaag gaactagaca
     3481 aaaaaactaa tgaaattgaa gctatctatc tgactcggaa ggcttctggc gacgattcta
     3541 acttgtcggt cttacgttcg cctattggaa ggataccgag tagccgggga gccttgcttt
     3601 aggcaaggtc ccaagccctc tccgaaccgt acgtacacat tgaatgtata cggctctcca
     3661 ttagaagtcg aacgcgaggt tcgagttttt cttttgtcga acaagatcat taatgacacg
     3721 tttgggtctt attccttgag tgagagttcg cagtatccgg taaaggatat tccatggtac
     3781 aggccttcgt tgtctgcgga acttgcgacg aaggtatctt gccagttgtt gtctgacgac
     3841 attgacttgc cgatagaaaa tccgattggc attgctgtat ctgaaatact cgctccatcc
     3901 gtcccagatt tctttggcct ttttcttggc ctcagccaga ctcaagtttg gcattaagct
     3961 ttctttgagt ttgcgggaaa gattcttacg tgatttctcc gaaggctgtc ttgcgaccca
     4021 gaatcttttt ggacagtcct cgaaggctct gaggtggaac ttaaatccga ggaaattaaa
     4081 atgactttca tggccgcgga aacggttttt catgtttacg agtctggttt tactttcctt
     4141 taaattgagt ccgacccgct tgagctcagc tttgatcatt cgtagcagga actcggcact
     4201 ttgctttatt tttgctagaa caacgaagtc gtctgcgtaa cgaacgaggt gcgcgtcttt
     4261 gaggttccac tcagcaaact ttttatccaa gctgtagtgc agataaatat tggccagcag
     4321 tggcgagagg gggcctcctt gcggagtacc ctctggcgtg ggctgccaag gtttaccggg
     4381 ttcaataaag cctgccttca ggatttgctg gattaacttc aacagacgag gatcggtgat
     4441 ctcatgtttg agcattccca tgagctttcg gtgctcgacg cggtcaaaga aggcctcaat
     4501 atccgcatcg ataacatgac gatgatgata gcagaggtca gccactttgg ccgcacagtc
     4561 tctggcgctt tttgctggac ggaatccgta agataaatcg aggaaccgca gttcgtagat
     4621 aggctcaaga attaaaacca acgctctttg gatgacccgg tcgcggagcg ccggtatacc
     4681 caatgggcga aggctttgat cactctttgg aataaacact ctttttactg gcaggggttt
     4741 gtatgatttg ctcttcatct ctgccgcgag ttctttgacg aagccccatt catggccttt
     4801 gatcctatcg atggtgacgc gatcaatacc gcctgtgccg tcatttttga taacgagact
     4861 caaggcctcg gccaaaaggt aggggtccat caagtgtcga tagaggtccc attgttttcg
     4921 gttctgattt gtttgatttc ggatcttgtt ttgaagagtc aaagtgtgcg gagcgagaga
     4981 cgttttcttc tgctcccggg gagttgactt agaataggaa cccataccca acacctgcct
     5041 ttgctaaaca agacttctaa tgacctgtcc atcccttccc ccagcgtcac cccttttaga
     5101 gcattgattg agtgagcgag atcttccttg aatgtccttg gctttcccaa ggtccgagta
     5161 ctatggagga ctctgacttc tccttcatcg ggttgcttcc cttattgctt cggggttggc
     5221 cctgtgtcag cttcaaaccg tctgatacgt gatgaggaga tctcttctgt tatctgaaac
     5281 aaccctgaat cacccgccat cttccagcac gagagagagc gaatctagga tcacaccgct
     5341 tttaagttct ggaagatatc ttccaggtcc tgacgatgga gggtcatcct tcctattgga
     5401 aatcccagat ttatggagcc ttccacgtca ccaaccgctt gggcctgaag gatcgtctgc
     5461 tgatcctttg tctctagctt cctgataact cgtgtttaca gaagttcaca ttgttatggc
     5521 cgatgattct atctggcccg catgtacttt agcggcgccg gtactgtggc aatttgattg
     5581 gtctcgtcgc cgatccccaa ccgccacagg atttacttgg tggaatgacc tccttccaag
     5641 cagaaataca ttccctgggt tccttccgg
//
LOCUS       DATBTE010000020         3909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_958970, whole genome shotgun sequence.
ACCESSION   DATBTE010000020 DATBTE010000000
VERSION     DATBTE010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958970"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VIG33_00600"
     CDS             <1..525
                     /locus_tag="VIG33_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8269359.1"
                     /translation="HFYKSIFGFEEVRYFDIRGAKTGLTSYALRSPCGKFCIPINEAS
                     ESKSQINEYLEEYKGPGIQHLAFLTDNILDSLDKLKGSSINMLDIDDDYYREIFKKFP
                     QVTEDHQKIKGYNVLVDGDEKGYLLQIFTKNLVGPIFIEIIQRKNHNSFGEGNFDALF
                     RSIERDQMKRGFLT"
     gene            522..1238
                     /locus_tag="VIG33_00605"
     CDS             522..1238
                     /locus_tag="VIG33_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEY8269360.1"
                     /translation="MKKVLFKKFGPTEDRTWKALFARQSLKRDLQIVPQFSQGLKALG
                     HCAETIPHLENVNSRLRKLTGWEGVPVEGLEEGASFYDMLAQRKFPIGNFIRDEKDLS
                     YTPAPDIFHDLYGHLPFFVDPQYAEFCQEYGRQASKYKNDPKILQEFERFFWFTIEFG
                     LIETAEGRRIFGAGIASSYGECEYALSTRPIIKNFDVEAIRNQEFRIDIFQEQLFVLK
                     SVDQLYGSLDIFVKAYKAAH"
     gene            complement(1235..2500)
                     /locus_tag="VIG33_00610"
     CDS             complement(1235..2500)
                     /locus_tag="VIG33_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8269361.1"
                     /translation="MNYVLQAVFHTPSVKMLACFGTLSFCSVYLVELFLTSQKKSFKI
                     FPYIIMSIFSVLLGALIFETTGNSQWALIPIAISVASPLLQTAFRTLIQYKSNDFYQR
                     FFAILLFFNGLEYLAYPFLRRIEPLGLTLSISLAHIILFTMSVFLPAFILRNNYKDNI
                     AELEKEVAKRTFSLTEKNRELLELSRENLSLINIVCHDLATSISILNANCFYINIRLT
                     IAEKEKYGSHLEMIQRTLKIINEIFEKVKVLEAVKTGKIHLELTPVNLLEELRESVHL
                     FNDLISEKKLQLRLDCNLPQDTLVLADPVILRNQIINNLISNSIKFSQENGEIEISAI
                     AENNKVYLHIRDHGIGIPAKMLPNLFSFESPTTRQGTKGEKGTGFGLTLVKVCVEKFG
                     GHISVKSETVEDGASDHGSTFIIELKRAN"
     gene            2811..3245
                     /locus_tag="VIG33_00615"
     CDS             2811..3245
                     /locus_tag="VIG33_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8269362.1"
                     /translation="MLDIRKKVFVCDDDSDMVHVICDIMVYMNLESTPCSDSRRAIET
                     IEKTKPDIIISDIQMPIFSGLDLIRSLNEKNYRIPIIFLTGRNEIKIYHETFVRGHFE
                     FLKKPLDPYELIKTVDKALNFGYSPPIENRFFQNLKKASIDE"
     gene            complement(3246..>3909)
                     /locus_tag="VIG33_00620"
     CDS             complement(3246..>3909)
                     /locus_tag="VIG33_00620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEY8269363.1"
                     /translation="AFVLAEEKYQVPDNVIAALLWIETRHGENLGHFHIVSVYLHLLQ
                     SDLRSNKKALTLLAIEKNKKVLEYPEEDIKSIMAERTKKRTLWAREQLLALASIRKKK
                     HLDLKKLRGSYAGALGLPQFIPSSYQDFAKSIKPKAAANLNAPGDAIMSVAYYLSKHG
                     WQNCDAESKIAALMKYNNSRDYADSILEISKRTAAQNHIPNARRISSTDDLATSDHCK
                     AE"
BASE COUNT         1177 a          689 c          826 g         1217 t
ORIGIN      
        1 catttctaca aaagcatttt tggttttgaa gaagtgcgct actttgacat ccgtggagcc
       61 aaaaccggtt taacctctta tgctttgaga tccccttgcg gtaaattttg tattcccatc
      121 aatgaggcga gcgagtctaa atctcaaatc aatgagtatt tagaagagta caaagggccg
      181 gggattcaac atttggcttt cttaactgat aatattttgg attctttgga taaattaaaa
      241 ggttcttcca ttaacatgtt agatatcgat gatgattatt atcgagaaat ttttaaaaag
      301 tttccccaag tgacagaaga tcatcaaaag attaaaggct acaatgtttt agtggatggt
      361 gacgaaaaag gatatctgct gcaaattttt acaaaaaatc tagtaggtcc catctttatt
      421 gaaatcattc aaagaaagaa tcacaattct ttcggggagg gaaactttga tgctctcttc
      481 cgctctattg aaagagatca aatgaaaaga ggattcctta cttgaagaaa gttcttttca
      541 agaaatttgg ccccactgaa gaccgaacgt ggaaggctct ttttgcgcga caaagtttaa
      601 agcgggatct gcagatcgtt cctcaatttt cccagggttt gaaagcgctg ggacactgtg
      661 cggagaccat tccgcatctt gaaaacgtaa atagccgtct tcgcaagttg accggctggg
      721 aaggtgtccc ggtcgaggga ctggaagagg gcgcaagctt ttacgatatg ctagcccaaa
      781 gaaagtttcc aatcggaaat ttcatacgcg atgaaaagga cctatcctac actccagctc
      841 cggatatttt tcacgatctt tatgggcact tgcctttttt cgtggatcct cagtatgcgg
      901 agttttgtca agagtatggc cgacaagcaa gtaaatataa aaatgatcct aaaatcctcc
      961 aagaattcga aaggtttttc tggtttacga tcgagtttgg gttgatcgag accgctgaag
     1021 gacggcgcat ttttggtgcg ggcatcgctt cctcttacgg tgagtgtgaa tatgctcttt
     1081 cgacaagacc catcatcaaa aactttgatg tggaagcgat tcggaatcag gaatttcgta
     1141 tcgatatttt tcaggaacag ttatttgtct taaaaagcgt cgaccagctt tatggaagcc
     1201 tggatatttt tgtaaaggcc tataaggcgg ctcactaatt agcgcgcttc agttctatta
     1261 taaaagtgct gccatggtca gaagcaccat cctctacggt ttcactcttg acgctgatat
     1321 gtccaccaaa tttttccaca cagaccttca ctagtgtaag gccaaatcct gttccttttt
     1381 cacctttggt tccctgacgt gtcgtaggac tttcaaaact gaaaaggttc ggtaacattt
     1441 ttgcaggaat accgatgccg tgatcacgga tgtgaagata aactttgtta ttctctgcga
     1501 ttgcggaaat ttcaatttct ccattttctt gggaaaattt aattgaatta gaaatcaaat
     1561 tattaataat ttggttccgt aaaatcaccg gatccgcgag gactagggta tcttggggaa
     1621 ggttgcaatc taaacgcaat tgcaactttt tctctgaaat caaatcatta aaaagatgta
     1681 ctgattcccg cagttcttct aaaaggttga cgggggttaa ctccaggtga attttccctg
     1741 tttttacagc ctcaagaact tttacttttt caaaaatttc atttataatt tttaatgttc
     1801 gttgaatcat ttcaagatga gaaccgtatt tttctttttc cgctatggtt aaccgaatat
     1861 tgatataaaa gcagtttgcg ttcaggatgc tgatcgatgt ggcaaggtca tggcagacaa
     1921 tattaattaa agacaggttt tctcggctga gctcaagcag ttctcggttt ttttctgtta
     1981 gagagaaagt tcgcttggca acttcctttt ccagctccgc aatattgtcc ttatagttat
     2041 ttcgtaaaat gaaggccggc aggaaaactg acattgtgaa gagaataatg tgagccaaag
     2101 aaatgctaag agttaaaccc aacggttcaa ttcttcttaa gaaaggataa gctaagtact
     2161 caaggccatt gaaaaacagc agaatagcaa agaagcgctg ataaaagtca ttggatttat
     2221 attgaatgag agttctaaag gcagtttgca gaaggggaga ggcaacgctg atagcaattg
     2281 gaattaaagc ccattgggaa tttcctgtag tttcaaagat caaagcccct aataggacag
     2341 agaaaatact cataattata taagggaata ttttaaagga ttttttttgc gaggtaagaa
     2401 aaagctctac gaggtagaca gaacagaagg aaagagtccc aaagcaggct agcattttca
     2461 cgctgggagt gtgaaatacg gcttgaagta cataattcaa aaaatagctt aaccagattt
     2521 tgagaaggat tttatacagt ggatcgcgat tatgattcca taatgagaat gtaatgattc
     2581 tattaactaa cagagcaaat gcgtaagtgg tgaatattat atacagatag ttattcataa
     2641 tgcggtgtta cctcctgtat cgtctcggat tcccaagaga tttatcaagt cgagattcaa
     2701 tcgaatgggt ctagtctaga tcgtgaaaca taattataaa aatgtgttta taaatactat
     2761 tagtttaagt ttttttttat cacgacgata actaaatcaa tgaggtcttg atgttggata
     2821 taagaaaaaa agtatttgtt tgtgacgatg actcagacat ggtgcatgtt atctgtgata
     2881 tcatggttta tatgaatttg gagagtactc cttgtagtga tagtcgacgg gctatcgaaa
     2941 ctatagagaa aaccaagcca gatataatta tttcagatat tcaaatgccg attttttcag
     3001 gcctggattt gattcgtagc cttaacgaaa agaactatcg tattcccatt atctttttaa
     3061 cgggtagaaa tgaaattaag atttatcatg aaacttttgt tcgaggtcat tttgaattct
     3121 tgaagaagcc tttggatcct tatgaactga ttaaaacagt ggataaagct cttaattttg
     3181 gttacagtcc acccattgaa aatcgatttt ttcaaaattt aaaaaaagcc tccatcgatg
     3241 agtagctatt ccgctttgca atggtcagac gtcgctaagt catcagttga agatatgcgg
     3301 cgagcgttag gaatatgatt ttgagcggcg gtacgcttcg aaatttcaag tatgctgtcg
     3361 gcatagtcac gactattgtt atacttcatg agagcggcaa tcttggactc tgcatcgcaa
     3421 ttttgccaac cgtgctttga taagtaataa gctacgctca taatggcgtc gcctggggca
     3481 ttaagattcg cagcagcttt gggctttatt gattttgcaa aatcttgata gcttgagggg
     3541 ataaattgcg gaagccccaa ggcaccagca taggaacctc ttaatttttt tagatctaaa
     3601 tgtttctttt tacgaataga ggccagcgct aagagttgtt ctcgagccca taaagttcgt
     3661 tttttagttc tttctgccat tattgatttg atatcttctt ctggatattc gagaactttt
     3721 ttattttttt cgatggcaag aagagtgagg gcctttttat ttgagcgcaa atcagattgc
     3781 agtaaatgaa gataaacact tacaatatga aaatgtccca gattctctcc gtgcctggtc
     3841 tctatccaca gtaaggcggc aataacgttg tctggaacct gatatttttc ttcagcgagg
     3901 acaaaggcc
//
LOCUS       DATBTE010000021         3238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_139097, whole genome shotgun sequence.
ACCESSION   DATBTE010000021 DATBTE010000000
VERSION     DATBTE010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139097"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..134
                     /locus_tag="VIG33_00625"
     tRNA            59..134
                     /locus_tag="VIG33_00625"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:92..94,aa:Thr,seq:tgt)
     gene            579..1370
                     /locus_tag="VIG33_00630"
     CDS             579..1370
                     /locus_tag="VIG33_00630"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY8269364.1"
                     /translation="MKSTTQLIASLALTGLLGACSGHKPAVNTTTVKPQAHSERKTYE
                     TPSPKLPGQWTIEDTLQVYGPYATRELTPYFTKAKVSYPPREVTFIALKQEKKLELWA
                     RDSGEFRFIRDYDIQSASGVAGPKLRQGDNQVPEGIYRIEALNPNSHYHLSMKINYPN
                     EFDLLHAWQEGRADPGSDIFIHGRAASIGCLAMGDKAIEELFVLTAHVGAEKVKVVIA
                     PHDPRISPLEADSEALPEWTPELYSLISHEIKALSPVVKSAKIGL"
     gene            complement(1496..3112)
                     /locus_tag="VIG33_00635"
     CDS             complement(1496..3112)
                     /locus_tag="VIG33_00635"
                     /inference="COORDINATES: protein motif:HMM:NF021594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
                     /protein_id="HEY8269365.1"
                     /translation="MGSTLSVGKTTVLKLVDFCLGAKPKIIYEDPENTKEIYTLVRDY
                     LVENKVSVCLHLTEDLDEEEARSVVIERNFLSKKNIVRKIDGISYTESEFEDKLTELF
                     FPGQVGKKPTFRQIISHNIRYSDLSVSNTLKTLDRYTSDAEYETLYLFLLGCDFSKGS
                     NKQEILQKIQQENVFKKRLEKVQTKSAYEAALALIETEIKDLDKKKSALNVNENFEED
                     LNSLNKLRYEINLMSSEIGNLNIRKNLIEETKNELSSAASNIDLSQLELIYKQASNNI
                     DSLQKTFEDLVVYHNQMVNERILYISKELPGLEEKIRRKDADLRKLLKRERELAELIS
                     KSDSFEELENLISKLNTKYRDKGEFESIISQLDEVEENIKTFESDLNEIDNELFSDDF
                     DAIVKQQVNKFNKHFSATSEALYGERYALKYDKIVNKKGQKLYKFSAFNTNFSSGKKQ
                     GEISCFDIAYTLFADEENIPCMHFLLNDKKELMHDNQLVKIADLVNKNGIQFVASILK
                     DKLPDDLNNEDYFIVKLSENDKLFRIENEQ"
BASE COUNT          860 a          780 c          637 g          961 t
ORIGIN      
        1 agaaagccga aaaagtacta gccaagaaaa aaatgttgtg tataataccc gcttcttcgc
       61 tggtgtagct cagtcggtag agcagctgat ttgtaatcag ccggtcgcgg gttcgattcc
      121 catcgccagc tccatataaa ataaagggaa tcaatgacat acaacgtctt gattccattt
      181 tttattgccc taatttttcg ttgcgtgact tttgcgtgac tcattctccg cagacttttc
      241 ccgccgcgat taaaaatcct cgtcgaatat cttgaaaggc ctgagtcact gtgcaagatg
      301 aagtcttgcc ggaaaccgct gaaatcttcg tttacctagc catgacctgg aaaacggctt
      361 tcaatattcg cccctattga gtactgagtt ttccagtcat aaatttacat gggtaatact
      421 atcaacatga ccgtggttgg ctgctggaaa atttacacac tccttttgtt ataaaagtga
      481 caaattttag acgccgtttt ccagctaaga ccccgaactt gcagaggttg ggcggtttta
      541 cctacactat accggtatcc actcaattcc agcgcattat gaaatcaacc acccaactca
      601 ttgccagctt ggccttgaca ggattgctcg gcgcctgctc cgggcacaag cccgccgtca
      661 acacgactac tgtcaaaccc caagcccatt ccgaacggaa aacgtatgag accccgtctc
      721 cgaagctgcc tgggcaatgg accatcgagg ataccctgca agtttacggc ccatacgcga
      781 cccgcgaact caccccctac ttcaccaagg ccaaggtgtc ctacccaccc agggaagtca
      841 ctttcatcgc cttgaaacag gagaagaaac tggaactatg ggccagggac agcggcgaat
      901 tccgtttcat ccgcgactat gacatccagt ccgccagtgg cgtggcaggc cccaaattgc
      961 gccaaggcga caaccaggta ccggaaggca tttatcgcat tgaagcgtta aatcccaaca
     1021 gccattacca cttgtcgatg aaaatcaatt accccaatga attcgacctc ctccatgcct
     1081 ggcaagaggg gcgtgccgat ccgggctcag atatcttcat ccatggcaga gccgcctcca
     1141 tcggttgcct agccatgggc gataaagcga tagaggaact tttcgtcctc accgcacacg
     1201 tgggcgccga aaaggtgaaa gttgtcattg ccccgcacga ccccaggatt tcccctctgg
     1261 aggctgactc cgaagcactt ccggaatgga ctccggagct ttattcgctc atctcccacg
     1321 aaatcaaagc cctgtcaccc gtggtgaagt cggcaaagat cgggttataa cccatcaagg
     1381 ctggttgttg ttagccagcc tatcctacgg attcattcaa cttcgaccga gcactaccgg
     1441 caataagcgg acatttacag gtcaagacct gacctgaatg acattagaat catcattact
     1501 gctcgttttc tattctgaat agtttgtcgt tctcggaaag cttcactatg aaataatctt
     1561 cattgttgag atcatccggc aatttatcct tcaatatcga agccacaaat tgtatgccat
     1621 tcttattcac aagatcagct atcttcacga gctggttgtc gtgcatcagc tcctttttat
     1681 cgttcagcaa aaaatgcata caagggatat tctcttcatc agcaaagagg gtgtaggcaa
     1741 tatcaaaaca ggaaatttca ccctgctttt tgccggagct gaaattagta ttaaaagcgc
     1801 tgaacttgta aagtttctgc ccctttttgt tcacgatttt atcgtatttg agagcgtatc
     1861 tttcaccgta tagcgcctcg gatgtcgctg aaaaatgttt attaaacttg ttcacttgct
     1921 gtttaactat cgcgtcaaag tcatccgaga acagctcgtt atctatctcg ttcaaatcag
     1981 attcaaaagt tttaatattt tcttcaactt catctaactg gcttataata ctttcaaact
     2041 cgcctttatc cctatacttg gtattaagct tggagatgag attttcaagc tcttcgaatg
     2101 aatcactttt agatatgagc tctgcaagct ctcgctctct ttttaaaagc tttctcagat
     2161 ccgcatcttt cctgcggatt ttttcttcga ggcccggaag ctcttttgaa atatacaaga
     2221 tacgctcgtt caccatttgg ttatggtata caaccaaatc ctcgaaagtc ttctgtagag
     2281 aatcaatatt gttggaagct tgtttatata tgagttctag ctgagataag tcgatatttg
     2341 aggcagctga agacaactca ttcttggttt cttctataag gttctttctt atatttagat
     2401 ttccaatctc gctgctcatc aaattgattt catatctgag cttattaaga ctattcaggt
     2461 cttcctcgaa attttcattg acgttcaagg cagacttctt tttatccaag tcttttattt
     2521 cagtttctat cagtgcaagc gctgcttcgt atgccgactt tgtttggacc ttttcaagac
     2581 gtttcttgaa gacattttcc tgttgtattt tttgaagaat ttcctgcttg ttactgccct
     2641 tagagaaatc gcaccctaac aggaaaagat acagggtttc gtattcagca tcgctcgtat
     2701 accgatcaag agttttcaga gtattcgaaa cactcaaatc actgtagcgg atattgtgag
     2761 aaatgatctg gcgaaacgta ggcttttttc cgacctgacc tgggaaaaac agttcggtaa
     2821 gcttatcttc aaattcactc tcggtgtaag atataccatc aatttttcga actatgttct
     2881 ttttagatag aaagtttcgc tcaatgacta ctgatctagc ttcttcttca tcaaggtctt
     2941 cagtcaaatg cagacacaca gagactttat tttctacgag atagtccctg accaaggtat
     3001 agatttcttt ggtattctca gggtcttcat aaataatttt tggctttgca ccaaggcaaa
     3061 aatctactaa ttttaatacg gttgtttttc cgacacttaa ggtactcccc attgtccaga
     3121 ccaaataatc atgtaattaa gatagaggcg ggttgataaa aagttgttat ttccgtttct
     3181 tcatgacgct tcgccaaata cacatcagct ggggtccgat agtccagcga ctgatgaa
//
LOCUS       DATBTE010000022         3277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_549451, whole genome shotgun sequence.
ACCESSION   DATBTE010000022 DATBTE010000000
VERSION     DATBTE010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549451"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /locus_tag="VIG33_00640"
     CDS             complement(<1..1545)
                     /locus_tag="VIG33_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8269366.1"
                     /translation="MKKRVDILIVDDRLDGLIALEAILTDPRYNLIKAQSGMEALSLL
                     PLHDFAIILLDIQMYGVDGFETASRIKQMENQKHIPIIFVTAMNKDENYIYKGYQAGA
                     VDYVFKPFDENILRSKVAVFVDLYEKSKRVEEQALLIRESANHQRHLRLAQLEVESLK
                     RYQNLADSIPHAVWRSKPDGTMDYFNKVWSEYTGLSDHQSLGFGWQSAFHPEDLKKFL
                     KIWMQKIANAEDFQVECRIRNRRGEMLWHLIRGIAECQGEKQIFAWLGTCTNIHERKD
                     MEESLIKARRAADAANVAKTNFLANMSHEIRTPLNSILGFTELLMTFPMSAKEREKNL
                     ATIKKNGSVLLKIIDEILDISKVEAGHLEIEQVELNLNRLYQDLYYLFAIQAHDKNIE
                     LTFSCSTPIPSKIVTDPTRYRQILVNIIGNSVKFTEKGKVAIEVSWLPDTTSIRGGLL
                     RCSIVDTGIGIDAAEAKELFQPFVQVDNSNTRKFGGTGLGLALSRRLSEALGGNVDIQ
                     KSEKNVG"
     gene            complement(1542..>3277)
                     /locus_tag="VIG33_00645"
     CDS             complement(1542..>3277)
                     /locus_tag="VIG33_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8269367.1"
                     /translation="SEVTLNLIASYAFTFRKGVSNKYLLKEGLVGQCAFERKQIILKQ
                     PSDNSNMISSSTVEVKPLNVIVLPVLFEGELKAVIELASVKNFNQNHINFLNQLMDSV
                     GVILNMISSSMRTEELLQELKRSNAELEAQAKELEEKAKLLGVKNQEVELASQSLEEK
                     AEQLSLISKYKSEFLANMSHELRTPLNSLLILSKTLAANKEKNLTAEQVKFASTVFSA
                     GQDLLALINEILDLSKVEAGKIPVSPKDLPIKEIEEYLEKTFRPVAEHKGLNLQIQID
                     EKLPKSIFTDESRLQQILKNLLSNAFKFTEKGGVQLQIEKGDKMEKYSLESLEQAKEI
                     ILFRVIDTGIGIPLEKQKMIFEAFQQADGTTSRKYGGTGLGLTISREIARLLGGFIEV
                     ESEPGEGSIFTLILPIRNTVTDNGNPNIDAPNASIKATSSVTAMNPLPVDADFRGFKI
                     LIIDDDLRNIFALTSVLRSHGMDVIYAENGLEGIQTLKGNSDTHLILMDMMMPEMDGL
                     DATKEIRKIEEFKNLPIISLTAKAMKGDREKCLDAGASDYITKPVDEIDLLAVMYAWL
                     KSEKRLHRSDS"
BASE COUNT          875 a          750 c          618 g         1034 t
ORIGIN      
        1 ccccacattc ttctcgctct tttgaatatc cacattccca cccaaggcct ccgacaaccg
       61 ccgagaaagg gccaagccca atcctgtccc cccgaatttt ctcgtatttg agttatccac
      121 ttgaacaaaa ggttggaaga gttctttggc ctccgccgca tcaattccga ttcccgtgtc
      181 aacaatagaa caacgtagta agcctccacg aattgaagtc gtatccggaa gccatgacac
      241 ttcaattgcc accttacctt tctctgtgaa ctttacggag ttacctatga tattcaccaa
      301 aatttgtcga tagcgcgtag gatcagtcac gatcttagag ggaattgggg ttgagcaact
      361 aaaggtaagt tcaatattct tgtcgtgagc ctgaattgca aataaataat ataaatcttg
      421 atacaatcga ttcaagttga gctctacttg ttcaatctca aggtgtccgg cttctacttt
      481 tgaaatatca agaatttcgt cgatgatttt taataagaca ctgccatttt ttttgattgt
      541 ggctaagttt ttttctcttt ctttcgcgga catcggaaag gtcatcaaca attcggtaaa
      601 gcccaatatg gaattcaggg gggtacgaat ttcatggctc atatttgcta gaaaatttgt
      661 tttagccacg ttcgcagcat cggcggccct tcgtgccttg atcagacttt cttccatatc
      721 ctttctttca tgaatatttg tgcaggttcc tagccaagcg aaaatctgtt tttcaccttg
      781 gcattcggcg atgcctcgaa tcagatgcca aagcatttcc cctctgcgat ttcgaattcg
      841 gcattcaact tgaaaatcct ctgcattggc aattttctgc atccatattt taaggaattt
      901 ctttaggtcc tcgggatgaa aagccgattg ccaaccaaat cccaaacttt ggtggtctga
      961 aagaccggtg tattcgctcc aaactttatt aaaatagtcc atagtaccat caggttttga
     1021 ccgccaaaca gcatggggga ttgagtccgc caaattttgg taacgcttga gactttcaac
     1081 ttccaattgc gctaaccgaa gatgtctttg atggttcgcg ctttccctta tcaacagtgc
     1141 ctgctcttcg accctttttg acttttcata aagatcaaca aacaccgcga cttttgagcg
     1201 caaaatattt tcatcaaagg gcttaaagac gtaatcaaca gcgcctgcct ggtaaccctt
     1261 gtaaatatag ttctcatctt tattcatagc cgtgacaaaa ataatcggga tgtgcttttg
     1321 attttccatc tgtttaatgc gagaagctgt ctcaaaacca tccaccccgt acatttgaat
     1381 atccaataaa attattgcaa aatcgtgcaa aggcaagaga ctcaaagcct ccattcctga
     1441 ttgagctttt attagattat aacgcgggtc cgttaggata gcctcaagag cgatcaaacc
     1501 atccaaccga tcatctacta ttagaatatc aactcgtttt ttcatgagtc actcctatgc
     1561 agccgcttct cacttttgag ccaggcgtac atgactgcca aaagatctat ttcatccacg
     1621 ggtttggtaa tatagtcgga ggctcccgca tcaaggcact tttcacgatc ccctttcatc
     1681 gcctttgcag tgagggatat gatgggaaga tttttaaatt cttcaatttt tcgaatctcc
     1741 ttagtggcat caaggccatc catttctggc atcatcatgt ccatgagaat aagatgtgtg
     1801 tcggaattcc ctttcaaagt ctgtatgccc tcaagcccat tttcagcata aatcacatcc
     1861 attccgtggg atcgaagcac actcgttaac gcgaagatat ttcgcaaatc atcatcaata
     1921 attaatatct tgaaccctcg aaagtcggca tcaacaggaa gaggattcat cgcagtcaca
     1981 cttgaagtcg cttttatgct ggcatttgga gcgtcaatgt tggggttccc gttgtccgtg
     2041 actgtatttc tgattggcag aatgagggta aatatacttc cttcgccagg ttcactttca
     2101 acttctataa atccacccaa tagacgcgca atctcgcgac taatcgtaag gcccaaaccg
     2161 gtgccaccat attttcggct tgtcgtacca tcggcttgtt ggaaagcctc aaaaatcatt
     2221 ttctgtttct ctaaaggaat tccaatgcca gtatcaataa cacggaataa tatgatctct
     2281 tttgcctgct caagagattc gagactgtac ttttccattt tatctccttt ctcaatctgc
     2341 agttgaaccc cccctttttc tgtaaattta aaagcatttg aaagaaggtt ctttaaaatt
     2401 tgctgtaatc tactctcgtc ggtaaaaata ctttttggca gtttttcatc gatttgaatt
     2461 tgaagattga gccccttgtg ttcagcgacg ggccgaaagg tcttctcaag atactcttca
     2521 atttctttaa tcggtagatc tttaggactg accggaattt tcccggcttc tacctttgac
     2581 agatcgagaa tttcgttgat taaagccaat aagtcttgtc ccgctgaaaa cacggtacta
     2641 gcaaatttaa cttgttcggc ggtcaagttt ttctctttat tggcagctaa agtttttgat
     2701 aaaataagta aactattcag gggggtccgc aattcatggg acatatttgc taaaaattcg
     2761 gatttgtatt ttgaaatcaa tgataattgt tcagcttttt cttcaaggga ctggcttgct
     2821 agctcaactt cctgattttt gactccgagg agtttggctt tttcctcaag ttcttttgct
     2881 tgagcttcca gctccgcatt agaacgcttg agttcctgca gcaattcctc ggttctcatg
     2941 ctacttgaaa tcatattcaa aataacacct acactgtcca tgagctgatt taaaaagttg
     3001 atgtgattct ggttaaagtt cttaacggag gccagttcaa taacggcctt caactcgcct
     3061 tcgaatagaa caggcagaac aatgacattc aaaggtttta cctcgaccgt gcttgaggaa
     3121 atcatattgg agttatcgga aggttgcttc aagatgattt gttttctctc aaatgcgcat
     3181 tgaccaacga gtccctcttt taataggtat ttattcgata cccccttacg aaacgtgaat
     3241 gcatagctag caatgaggtt cagagtcact tcgctgt
//
LOCUS       DATBTE010000023        47415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_891006, whole genome shotgun sequence.
ACCESSION   DATBTE010000023 DATBTE010000000
VERSION     DATBTE010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 47415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47415
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891006"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VIG33_00650"
     CDS             <1..251
                     /locus_tag="VIG33_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269368.1"
                     /translation="VVKDSLQVAFNGFADSTLNILVNFHLAVFDANEELARQQKIFCE
                     IIATGVGTEVRAIWSKSPLSGPFGKYKSSLPCSRPRAH"
     gene            complement(169..1620)
                     /locus_tag="VIG33_00655"
     CDS             complement(169..1620)
                     /locus_tag="VIG33_00655"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEY8269369.1"
                     /translation="MKLIKNPRGTSLVTVLMAVGIGAIVLAGITTMITDVFKSQRTAQ
                     SKDAHRELTVSIRQLLIDPVICSASFKGNNPAGTGYTKTQIVDAATPPNIKFQTGVNY
                     LNNLVTITDFEVRGFVADNPPVNDKVGKAELNIKMNKLGSTIGSPQMTTTIFLQATLD
                     ASYNLNSCYALGLADSLWQISPANMADIYYSSGNVGIGTTAPTSLLDLKGSAGANGLY
                     IRTTSSAPNSSPYGIEFSNNLAGTAHVGIEMDYLGNFRIEKDNSNVFISITQSGNVGI
                     GTNSPSNSKLEVNSTNTWAIVGNGSQHGVQGSTTNSNNFAVQGYNSAGGGSGALGYGN
                     WGVWCVQTNCGGNAGWTNVSDRRLKRDIQPLDGALEKILKLRGVSFHWKDQNRDRKEG
                     EKIGLIAQEVEEVFPQAVLTNSADKKLPGGSKMLTTGDLIGPIIQAIKELHGKFLNWQ
                     KTQENRFNALEAENKAMKTYICQKDPKAGFCSK"
     gene            1884..5645
                     /locus_tag="VIG33_00660"
     CDS             1884..5645
                     /locus_tag="VIG33_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269370.1"
                     /translation="MHLILLLSSVFLSVKPALAAASGFTYTGKILDSNNAPVTATSVI
                     FTVTVFDPAGKCWLYTEQRKLDLSQTAGTFSFEIGSSDTATLYGATPSFNNQSSGGPK
                     DLADLFNNKKSYVGLGASNGCTGTYDPSTSSDPNEGRLLSVYFRVGSSGTDQAIPPLR
                     ITPVPIAMQSLAVNGYGTGELLRVDQATVASAGISNNALSVAQYTEFWDLMNKASISY
                     LPVTGDVSLVGGNNKLTTFLGQALPSGPASNGQVLVSNGTAWVLQSMSGGSGTVTSVT
                     GTAPVVVGGTASAPVVSLPVATTSASGYLTSTDWNTFNLKLSSSLASGKIFIGNASGL
                     ASEVSVSGDATLDNAGALTLKNTGTAGTYGSASLVPIVTTDAQGRVTSVTTAAPLDAT
                     KLPLAGGTMSGAINMGAQDITNSGNITMASSKTLGLSSNAADPGSPSAGQVWYNSSVN
                     LIKFYDGSSVQSLGVAGAGITSLNGLTAGGQTFAIGTSGNSPAFSSATSTHTLNIPMA
                     SSASVTAGLISKAEYDAFTAKLGAVTNTATLAPTKIWIGDAGSKAQEFALSGDATMTS
                     GGVVSVDKTQSAAASKILQLTASSVAVTKGTDIGGASTGKVSLRYPSTATDTTFTFPN
                     SAGSANQFLQTDGSGSLSWGTPAATLPALASTQVWVGNASGAAAAVALSGDISSMDNA
                     GVVTANKTTTAQASKLLSLDGSGVGAMMGNQLNGLTSGSLTLQPAAITTNYSLTFPAA
                     QGAAGQTLSNNGSGVLSWASTLTNSLTSAKILVGNASGLATEVSVSGDATMDNAGVVT
                     ANKTTTAQANKLLSLDGSGVASSMGNQLNGSTSGSLTLQPAAITTNYSLTFPAAQGAA
                     GQTLSNNGSGVLSWMTPLSSSTSFVNGGNTFAGNSSLGNNDNYNLDIKTNNLSRMTIL
                     NSGNIGIGTTSPATTLDINGSLKVGSDPICSADKAGAMHWTGSTFEGCNGTSWSAIGA
                     MGTPAQNVVISFSGGNNQTVTLANNSPITNGDARAAWSVYTNLPNYLLGTLGTDKING
                     SNVTLNLSAATTCYLLHDPGWVAVDLTGWTLLESGMPYVSTSNNINVYSKNLGAGAYT
                     FSVYSAIYSCVGTAYNWAMNGSSLYYSGGNVGIGTTTPTATLDVNGEVKFGNTSSICN
                     ASKEGQQRYNSISKIMEYCNGTAWSPFGFTTTTTVSCTVAVAGGWNGCTATCAAGYNR
                     TGCSSSNDSAWSGYIDRGAVPSGSNACNCNYWINNGSTVSLECTAYCAK"
     gene            complement(5732..6367)
                     /locus_tag="VIG33_00665"
     CDS             complement(5732..6367)
                     /locus_tag="VIG33_00665"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY8269371.1"
                     /translation="MFSFFSSRRWALHNPLKSWTLSGLSLRECQLLVASMTDSEMKVS
                     WAHQKQWENWKPLGSEDCHVLFLFQDSDLVTLPALPQITQENDHEITVVRSASPAPPL
                     REPIMRKYIRFVATIPVEIVVGSQSFPTHTVDLSEGGFCFTDKLPDWVAGYFTVLLKT
                     SDETFEFNCFLAEDQKKDKFRAEVAPTTSEKVIEEFRLWLFAQYFPQVPVK"
     gene            complement(6377..7552)
                     /locus_tag="VIG33_00670"
     CDS             complement(6377..7552)
                     /locus_tag="VIG33_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269372.1"
                     /translation="MCTPLKPLLLLCVLLNLAHAKSVDSTQVDAEEAITDAESAKADA
                     TEAKRRAEEERKRLEKARSEAKNALEKARAVEANAKKEQVEAEREVARLKQETAENEK
                     QQKQAELQQQQFEARIKAAQEKVIQAKTLKEQAIEKRKIAEKSSGEVAGKMKNFEKES
                     QKAMEDAQKSQKELDEAKMRLKQTEQELAKTQLNSQKAVAQGKATEARVKVEMARLDA
                     EKSRAEAEIKKAQAAQTQAEGTVKKLDEDSKQSQEALAKTKDALEVEKKKLAEAKSNE
                     EKSRQSYAKAKAELDAKKAELQKVTAESTFEVNKSRKAIAEFDAQSAKADAELARLKA
                     EADKAKKERESIEEALEKAKSKADERAIRVDTAKAETETEKMMLETAKIRAETAGIK"
     gene            complement(7637..8791)
                     /gene="purT"
                     /locus_tag="VIG33_00675"
     CDS             complement(7637..8791)
                     /gene="purT"
                     /locus_tag="VIG33_00675"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF006766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HEY8269373.1"
                     /translation="MKKIMLLGAGELGKEFVISAKRLGQFVVAVDRYTGAPAMQVADA
                     FEVIDMLDGAEIERIVKKHRPDIIVPEIEAIRTEKLIELEKKGVVVIPTAEAAHLTMN
                     RDAIRDVAAKELKLKTANYAYASSPEELLAAADKVGFPCVVKPVMSSSGKGQSVAKTP
                     KQLGAAWNYACEGMRGDKKKVIAESFISFDFEITLLTVKQRNGKTLFVDPIGHRQEHG
                     DYRESWMPAKMKPALLKKAQKMAKKVTDRLGGAGIFGVEFFITKEDVYFSELSPRPHD
                     TGMVTLISQDLSEFDLHIRAILGLPIPDIKYYGPSASAVVLATKESAKPPQFSGVEKA
                     LELKNVDVRIFGKPSTRPNRRMAVALARSANIEKAKNLAIKAAGKIKVCE"
     gene            complement(8840..9466)
                     /locus_tag="VIG33_00680"
     CDS             complement(8840..9466)
                     /locus_tag="VIG33_00680"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY8269374.1"
                     /translation="MIYDISPLITSSLAVFPGDRSFQRRVSMDFAQGDPLSLSSIEST
                     LHLGAHADAPSHYHKEGVSIDQCSLETYVGPCQVIDASSLKGELTLEKLGLVDILAPR
                     ILFKTNSILDVNVWQEDFSYLSPELIDWLAAQQVKLIGIDTPSMDHSQSKSLETHQAF
                     FKNHISILEGLVLQAVSPGLYVLIALPLKIHGAEASPVRAVLLDQIHF"
     gene            complement(9463..10650)
                     /locus_tag="VIG33_00685"
     CDS             complement(9463..10650)
                     /locus_tag="VIG33_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269375.1"
                     /translation="MWENFFDQVRCADTLGFGTAWLAETHLSCQVQKENPGAVIPHFK
                     GEIGLNTDILQLAHLIFQQTKNLNVGSAIRNILCNGGPVAHAEAVKTFLSLHALRPNE
                     KRVLELGFAAGRFPFSYHAYGIKARDPVDEAAWEVVKNKVFQEATLIMLRLLRGDHFA
                     SKDIGPLYLRREDFRDQATWDKVYKLAKEDSRRWDGGKIGQAEQILHLPFFVFDKVGV
                     IPFEAPLELLRLTIGSHDPTTQRMANEILPTGVFNLSITPAEVIEETHCRMRKEFHSD
                     GGAWTRAHMPRTALIFVDATEGLSPQQQSARAREQSRKAWQSYWVAMEKTLDPKKVES
                     AVENSIAGNPEEVAQAIVKKYHPEDRLMLWFDFNNHDNEQIKKSMRVFMEKIKPRVEE
                     LLR"
     gene            10871..11668
                     /locus_tag="VIG33_00690"
     CDS             10871..11668
                     /locus_tag="VIG33_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8269376.1"
                     /translation="MVLDLANSLANKRALICGASQGIGEATAQTLAKLGAEVILVART
                     QEKLEAVKSQLATKGGQKHQVLALDLSLTDSLNHALDSLLATGPISIVVNNAGGPKGG
                     ALLEATDEEFLQGFQAHILASSLIAKKVVPGMKEQRWGRFINIISTSVKAPIANLGVS
                     NTIRGAMASWSKSLANELAPFGITVNNVLPGYTETPRLEALAQAAAQRQGKSVEEIKN
                     LWKAATPAGRFAKPQEVAETIAFLASSSAGFITGINLPVDGGRTPCL"
     gene            11659..13107
                     /locus_tag="VIG33_00695"
     CDS             11659..13107
                     /locus_tag="VIG33_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY8269377.1"
                     /translation="MSLREIKNFICGEYSAAQSKKRIDNFNPSNGKVYSTLPRSDAGD
                     IEAAVQAAQKAFPAWSRTSPEERSQILLKIAEGIERRIPQLAEAESTDQGKPVWLAET
                     LDIPRAAHNFRFFAGQILFPCNESHEFNEKAFNFSLRKPVGVAGLISPWNLPLYLLTW
                     KIAPALACGNTVVCKPSEITPMTAFLFCDILNEAGLPPGVVNMVFGLGVEAGEALVKH
                     PKVPLISFTGGTTTGKHLSSAAAPLIKKLSLELGGKNANIIFADADLEEAIQTSLRSS
                     FLNQGEICLCGSRIFVEASIYESFVSRFVSETKKLKVGDPRKAENFLGALVSKAHLEK
                     VQSYVKLAQDEGGKIETGFEDIPLTAEQQQGYYMRPTIITGLRSECRVMQEEIFGPVV
                     TVVPFGSEEEVVSMANSVEYGLASTLWTKDIQRVHRLARRLEVGTLWVNTWMMRDLRT
                     PFGGMKMSGLGREGGLHSLEFFTETTNVCIKY"
     gene            13128..14075
                     /locus_tag="VIG33_00700"
     CDS             13128..14075
                     /locus_tag="VIG33_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269378.1"
                     /translation="MATKNQGKIQGSSRGKIVAGCLAPHPPHLVYAENPPQNEPRSEC
                     GWETLRWAYEAMREDLKKIEHDVLVILSPHWRTYVGTHILATPHLKSLSVDPIFPNLF
                     RYHYDIKVDVDLAETIHEVCQARNIPTKLMQNPDFRVDYGTIVTGQMVNPAWDKPIVC
                     LSANCVAAYYNLDRMQALMMELGAATKEAIEKSGRKAILLASHSLSHRHFTVENEIPE
                     DMSKEHITNHSLYLWDMKMLKLMKEGKSKEMVELIPDFTEQTIAESDSGALTWMMKAM
                     DFPTYPAKVHGYGTVIGTGNAIVHWAPPLQAPSSTEKGL"
     gene            14075..15100
                     /locus_tag="VIG33_00705"
     CDS             14075..15100
                     /locus_tag="VIG33_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269379.1"
                     /translation="MMTQHKSNNPVLKGFVLPGLVQPLLTPDANPGYRKVRAAFDKVR
                     EEIEHSDADVLLIYSTMWPSILGHQIQAMPEPEWVLVDEEFHELGTIPYKLKIDSSFA
                     ECYNRHAKLRGLHSRTIAYHGFPIDSGSVVALKLINPNNRIPAVIVSSNIYADRAETV
                     VLAKAAVSALKEQGKRAIAVIISTLSNRLHSDFIDPKDDKIHSLKDQEWNLKILEFLE
                     KGRLEDVAQLSRQIQREARVKKVTNFKPFWWLSSVMGSHNRYTGQIYEYQPIYGTGCA
                     VVGLTPALQASRDLEFDEDAPDYYTGERNVLSASGESAADFSAMSNLYNNLDGEGQTE
                     EDDHENF"
     gene            15087..16190
                     /locus_tag="VIG33_00710"
     CDS             15087..16190
                     /locus_tag="VIG33_00710"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY8269380.1"
                     /translation="MKISDVYPKSPFGEKIPGIPTGRDVEWQPLIDYRRNGVSENTIH
                     GAIAWYSGDELIHSFGGNVLCYGRSMMKLFYMKVFAQELANQTSWEQKAISVSSHNGA
                     AEHIEAAQSLLAESEWGLMQTPLDLPLVQFGRQVRRPRRWYNNSSGHHAAILLGCRLK
                     GWNRAGYTLPNHEVYGAYLKVVQQYLGKTWSPLRVARDGDGLPTVAMTVNELAKCYAG
                     LSKNRHEDWIWESMIRHPDLVGGFNRLDTTIMKSCKGDVIAKEGADGLLGMAISHPEY
                     PQGLGVVIKIAHGWNPQATWYIARGILGSLGFELRNPYPLRRQKAFLVPQIAPEKYFS
                     RLESIPTWDEWDPDHDRWYMEESMGEDKGGFNG"
     gene            16183..16596
                     /locus_tag="VIG33_00715"
     CDS             16183..16596
                     /locus_tag="VIG33_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEY8269381.1"
                     /translation="MADIINAAKAPEPVGAYPHARRFGDLLFLSGVGPRKPGTKKIPG
                     VELDAQGNITSYDVVVQTESVIENIRIILEASGSSLEKIIDVQVFLTNMKADFKSFNE
                     VYAKHFTATGATRTTVEVGALPTPIAVEFKVIAAF"
     gene            complement(16651..17709)
                     /locus_tag="VIG33_00720"
     CDS             complement(16651..17709)
                     /locus_tag="VIG33_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8269382.1"
                     /translation="MFKELKMIHQIMPALFAATLFGASTPLAKVLIGEMDSWILAGIL
                     YFGSGIGLLAISAVQSLKAKSLPVFPNKKDFFWLMGATFFGGILGPVFLMKGLSLTTA
                     STSSLLLNMEGVLTSCIAWIVFKEHYDRRIVLGMMAIIMGGMILSWSSAGEFSLSSGS
                     FFILGACLCWGIDNNLTRMVSANDAVLITTLKSVVAGSTNLILALSLGRSLPAAGPLA
                     WGALLGFLGYGLSIVCFVLSLRAIGTSRTGAYFSAAPFVGATLSLFLFKNELSLQFIL
                     AAGFMGWGLWLHVTEIHNHEHEHEPLYHSHEHTHDDEHHQHEHLPAESFGKKHTHYHQ
                     HEKMRHQHSHFPDIHHRH"
     gene            complement(17804..19759)
                     /locus_tag="VIG33_00725"
     CDS             complement(17804..19759)
                     /locus_tag="VIG33_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269383.1"
                     /translation="MAGFSRLFRIFALSFVLLINFSRASFAEGGEHFFRSKPPRVEPF
                     FFLDFDWGTWTVNLTPYNPKPSSLSESESLSKFRRDRMIMDDYRDGLQKLTHGTLSLG
                     LGAKVAFWYRSFSDLLTRWWSYVGIMPIVGVNVESVRYFKTVAEARHGKGYLKVPGHA
                     SDLISWSQGDSITYICRGGLLFGATIGYSLVDAGAALVAQGTWETYIEKIEENKIYLK
                     ITKGKMATLSAFTAATIVELSVAGFKSRDDGFSFLYNLNTEVGRKAYEDAIRGNIIAS
                     EKIAEAVPTNYVEAAPVLKVSTFKNLSTGRLISAELGLPLIWNKTYSRGKVLSYSATD
                     FHVKNKVAKVNYGIYSEKSNYRFFNRQRETDVRFYGSHYTIEEQGKTDVGMFGRYGYA
                     YMNDHSSGEKLNGAVRKLINLTAMDELQVDIPQENLAYAAIDFNTTLSKEHTLHLMDL
                     AQREGKRAIYKVADRYLQSYSLSKDVYGYCKGIHPQCWLDLRDDSYNAIEKMYRALLE
                     MYTEKNRDAKAFARAYGEFGEAMAKNFITFRTVLELAGPGVEINYLIEGARMSMYYKS
                     WTTTEVPNQWSVANNPNDRGKSSDKNSELPFEPRLHRSKTRGIIVNPNHGGLVFLQAP
                     VKFGIAGLPLTLDTSLNEVLNANYNLN"
     gene            complement(19857..22040)
                     /locus_tag="VIG33_00730"
     CDS             complement(19857..22040)
                     /locus_tag="VIG33_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8269384.1"
                     /translation="MQFLVPQISISIITSFVMTVLYFYLHTQDRKSFLYLWIRSGFAF
                     TLASFLQLLMVLKVMPEWGLIFRHAAMVSSALLLANGLSFLVTERSLKQLRIWIAVGF
                     LTTLLSALQEAPVFLFLGALYVWSGMLLFRSSLQNSLARRIVGGSLLLGGLYCGVVAG
                     LGPTKPFLFGGELLSFLLLLSVSLGLLGIYLDKIRSTFRLEINECHHVQERLKAIIQH
                     QPACIKTVATDGTVLAINPAGLAMLEASQCPEKIIGRNVIEFICPEDREAFLDLHQKA
                     CAGQVGIAQFKVIDLQGKSHWMESYSAPLHFSNEKTKAVLSVTHDITERKLTEQKLIE
                     SYEELKNAKLEAEAGTKTKSKFLANMSHEIRTPIGAILGYAELMLKRDLKVSERMDYV
                     NTILCSGRQLLEIIDEILDISKVEAGHLEIEILDVNLRHLLKDLWALMRIKAEEKGIP
                     LNFCFKSLIPEHIFCDPIRLRQILLNIISNAIKFTEKGQVTVEAYWIEGRLKFRVLDT
                     GVGIEADEIDKLFQPFSQVNNSISRRFGGTGLGLVLARTIGRSLGGDVYLESSEVGRG
                     SVFVVEVKAAAVENTLFIADIEMVLPRSLQIMNEAEEARLLEGISVLLVEDSPINQTL
                     ISRFLTVAGAKIDFANNGIEGLEKALTKNFDIVLMDIQMPEMDGYEATTKLRQQGYQR
                     PIIALTAHAMKEEREHCLNVGMSDFLTKPINRSLLISQILNYLHH"
     gene            22163..24856
                     /locus_tag="VIG33_00735"
     CDS             22163..24856
                     /locus_tag="VIG33_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEY8269385.1"
                     /translation="MNFEISWHALNVDEVVDQQKSHIIQGLSSEEIKQRLSKFGKNSL
                     PEPKRRSFLFIFFHQFLSPLIYLLLGAAGIAFFIHKAKDALVILIVVLLNAIIGAFQE
                     GRAENSLEALRQLSKLKARVLREGKESLIEASELVPGDILILNPGDAVPADARLITAS
                     SMAAAEAALTGESVPTPKSISPLPSTTALVDRHNMVYASTYITAGRGQAIVVATGIST
                     EIGKIADLTTTTLQPKTQLELRLEQFGRMLVITALIVFSLVLGLGLLHGIPFAQIFMI
                     AISQMVSLVPEGLPVAVTIAMAIGVQRMARRGTIARRLTAVESLGSISVICTDKTGTL
                     TRNEMTVTTIYLPANNRKLSISGIGYAPEGQLTPEPDNTLKKLFEICCLCNDAHLLTP
                     DIAGQWQILGDPTEGALLTLAAKGGVDSTLMRKEFPRIAELPFNSDSKMMATQHEIGG
                     KSVVFIKGAPESLLELCDSIYHEGSLKDFSKNLREQVENEVSKMAEKALRVVAFGFIE
                     NAFIDGSKGFSPFAQKMTLVGLVGELDPPREDVAESIRRCQMAGIRPVMVTGDHKATG
                     LAIAKALGISRNNDLIIDGQELDQLSDEDLAAKMDHISVFARVHPAQKLRIVKAYQKK
                     HEVVAMTGDGVNDAPALARADVGVAMGITGSEVAKEAAKIIITDDNFTTIVAAISEGR
                     LVYQNIKKLLLFLFVTSVDEVTVLFLALIFGYPPPLAAVQILWINLVTEGALTVNLVM
                     EPPEGDEMQRPPIPAKQPLLDSTLLSRIPFMGLASVISTFGWFVYRTSLGVTPALVQT
                     ETFTVLAICQWFNVLNCRSALRSVFSRDSFGSLRKNPWLLGGLALGITLHVAVIYWPP
                     LGQFFYTMPIAPAQVLQIVLVSSLVLWSEELRKFIMRRHLL"
     gene            24947..25648
                     /locus_tag="VIG33_00740"
     CDS             24947..25648
                     /locus_tag="VIG33_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269386.1"
                     /translation="MKSLFLSLLLILSSVILSTASHADQSATPSTSPGFEDVLVRDKK
                     DLDADSNMGLTARELEANRLMFKQKVEEFAMNAFRTPEGRPIIQAEANLLVKYFIELK
                     LPPEALTSYTFHSDLKLPFVGNEAQIKAQRSQVIQTLVNIGQTRTDMDTYLVEDDVRC
                     FYRNFRDTDAVEEVLKLSENAKMMNPLADIFRRSDDVVIVGKCQGCGLLNIKGYPKTP
                     EEEMNPFSACAGDSY"
     gene            complement(25656..26666)
                     /locus_tag="VIG33_00745"
     CDS             complement(25656..26666)
                     /locus_tag="VIG33_00745"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEY8269387.1"
                     /translation="MKIAFFDTHAFERNIFQKENTEFKHEITFFETRLTEQTANLAAG
                     YPCVCAFVNDRLDSKTLRIIAASGTRLIALRSAGFNHVDLEAASDLDLRIVRVPEYSP
                     YAVAEHAVALILSLNRKIPRAYNRVREGNFSLDGLVGFDLHKKTAGIVGTGKIGSVMA
                     KILAGFGCQILASDRVQNPELIEVGVQYVSFEELLQKSDIISLHVPLTPETRHLINGK
                     ALSQMKAGVMLINTGRGALIDTPALIQFLKSKHIGSAGLDVYEEEEGIFFENLSEQIL
                     QDDQLARLLTFPNVLLTSHQAFLTEEALVNIAQTTLRNITEFANGKNLSNEVHAEKHL
                     KK"
     gene            complement(26690..29113)
                     /gene="ppsA"
                     /locus_tag="VIG33_00750"
     CDS             complement(26690..29113)
                     /gene="ppsA"
                     /locus_tag="VIG33_00750"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEY8269388.1"
                     /translation="MEKSNSKSSPKFIKWFEEISIKDIPLVGGKNASLGEMFQKLTPQ
                     GIRIPPGFAVTAEAFSVLIEQNKISEEIYSRLARLNINDNDELARTGHEIRSLIRKTG
                     IPESVAQEIREAYTQLCLRTDIKELDVAVRSSATAEDLPDASFAGQQETFLNIRGAED
                     LLVACLNCFASLFTDRAISYRTGKGFEHAKVRLSICVQQMVRSDLAASGVIFTLDTES
                     GARNVILINSAYGLGENVVGGRVDPDEFFVLKPLLGKATLPLLRRKLGAKQVKMIYSG
                     HGSRTTRNIEVSQADREKLSLVDGDVIKLAEWALAIEQYYSKVHGHDTPMDIEWGKDG
                     RTGDLFILQARPETVHSQESTVQQSSFVLKERSKVLISGRAVGSKIGAGAVRIIKDVS
                     ELGQFKNGEVLVADMTDPDWEPIMKKASAIITNRGGRTCHAAIVSREHGVPCIVGSGI
                     ATEVLKDGQTVTVSCAEGDEGFVYEGALSFVEDKVNWTSLQKPHTKIMVNLGNPAQAL
                     KTSLLPVDGVGLARIEFIISEYVKVHPMALAHLEEIKEPEIKAQIENLLGSSKNNPAQ
                     YFIEKLAEGVGLIAGAFYPRPVIVRFSDFKTNEYAGLLGGQQFEPSEENPMIGFRGAS
                     RYYDSHYRDGFALECRAIKHLREQVGLTNIKVMIPFCRSPEEGEKVLAEMKRNGLVRG
                     EKGLEVYVMAELPSNVLRAEDFARIFDGFSIGSNDLTQMVLGVDRDSSVVSHLFDERD
                     PAVLKMLHLVIEGAKKSKRPIGICGQAPSDYPEISQLLVKEGISSISVTPDAVTKTFQ
                     VVLEAEKKN"
     gene            complement(29247..31844)
                     /locus_tag="VIG33_00755"
     CDS             complement(29247..31844)
                     /locus_tag="VIG33_00755"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEY8269389.1"
                     /translation="MVCFQNRIEAGIILADKLLHSLKQGTKGGKKEKPLVLAIPRGGV
                     PVAYEVAKKLQADLDLLIVKKIGTPENPELAIGAVSEEGTPWLNSKIINHFNIKNADI
                     QAMVEKKLEEVQSQVRRLRGSRPAIPVEGRVLIIVDDGIATGATLRAAIHLLRERQPE
                     KIIIAVPVAPEATLESLRQIADEVVCLETPFPFIAVGDWYQDFSQVSDEEVISLLHGK
                     VVPDEIVGKEMTYYDGTIELKADLQLVANMKGLVVFIHESEGSRHSRGNLQVAEELNK
                     IGFATLVPALLTERESEDRKNIFNMELLIDRLLKVSDAVIPHLAQVHGVHSSLAYFGT
                     GTGAAVALGAAAKSSRDIFAIISHGGRPDLVNKYLRRVKSPTLLMVSEADLEVITLNE
                     LAAQRLATVQMVIIIELEAIAEVVEYTADWLLQYAPRPKAPLPPKEHIVRAIEERAQP
                     IHSEGAWYNLIEKLAKSRIVMLGEATHGTAEFYDIRRMISEKLIRDHEFDFIAVEGDW
                     PDSQKLNDYIQGKSSSRTAMDIMHHFQRWPTWMWANDEVAALIEWMQNYKAGFYGLDV
                     YSLFESMDYVQSYIQRISPELAQEMKAAYSCFEPFEKNEKAYAQSLLKFEEGCRREVV
                     ENLRKILRLRLADLEVKDGDLFSAQQNARIVKNAEEYYRAMLSGGAESWNIRDQHMME
                     TLELLLNRPFQNRKCIIWAHNSHIGDYHATDMLEEGYVNLGGLARERFGSEQVALVGF
                     GTYQGEVLASSAWDGPETVTPLPAAQENSFESYCHKVSQNLQAPRFYMLFDQDARKTI
                     LGTREYGHRAVGVVYHPRFEVHGRNYVPTVMAKRYDAFVFVDQTTALRSIPTLTSIKE
                     FPETWPGGL"
     gene            complement(32410..33216)
                     /locus_tag="VIG33_00760"
     CDS             complement(32410..33216)
                     /locus_tag="VIG33_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8269390.1"
                     /translation="MKNSSSRQSAPICAPICAPILELIDVTLELGGEIILDGVNLSIH
                     QDDTIVIIGPSGGGKSVLLKTMAGIYTPTTGKVLCYGHEWEALSMSEKHDVARKIGVQ
                     FQKSALFDELTAFENVAFPLREHTRDSDEVIGDRVLACLKAVGLEAAKSLLPHEMSGG
                     MRQRLGIARAIALKPEILFLDDPTAGLDPVNSDLMAEMILNLKKEVAATLIIATHDMQ
                     MAYQFAGRILLVADKKVIETGNAVQTQNHPDPRVQQFIHGWLKGPLTEKY"
     gene            33298..34080
                     /locus_tag="VIG33_00765"
     CDS             33298..34080
                     /locus_tag="VIG33_00765"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEY8269391.1"
                     /translation="MQRMHFISGLPRSGTTLLSTVLNQNPKFSASISGPAARMVRAIV
                     TEAQAQGGYRTQCPEGKRRKLIRNLLETYYEDSREVAFDTNRGWTLMTPLLRDIYPHA
                     KMICCVRDIPWILDSFEQLLRKNPYSVSSLFSSEESVDVYSRTSTLLRADRTIGFALN
                     GLKQAVYGGERDMILLVNYDDFCRTPEGVMRDIYKFLGESYFAHDFNNVEASYDEFDQ
                     DVNLKGMHKVRKELTHIVREPILPPDLWHQADTLSFWKAGRI"
     gene            34098..35711
                     /locus_tag="VIG33_00770"
     CDS             34098..35711
                     /locus_tag="VIG33_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269392.1"
                     /translation="MEKQANSSAQTSRELGLAAAYASKWMEALLHLNAAVRQGHYDLM
                     VLDALGEAAYRANVPEALGPYQNHYKYPKIASHMARAFLMLGDVTSSYEFLGYAQDSA
                     LKSALFAMLELGSEITTTAAQMLPVAETHPDLYYPEYWRALSAVADAVGREDLTRLSE
                     RKSKDFAYKDPNIHFNQALRMLGKGEFRAGWRLYEWRLVPNAAQSNRTELGRIAMWEG
                     EHLRGKTLLIYLEQGLGDGIFALRYIPALLEQGVCLEVVARPALLPLIQASHPNVKIH
                     NEDEVVVVGYWDQVPSPDFWVYGLSIPFRAGFWRPMNTAKFLKAPTTVVEKFKERILR
                     QNLLHLPVYTVNWHGRIDTESDRSRAFSVQEFTQVTGLEKNPCLVISLQKDASEEEIT
                     CLQELVGKSGGQFINAAPELGDFAATAAWIAASDRLLTCDTSVAHVGGALGHPTTVYV
                     RNKAIWQWLRKDPEKTSAALWYESALVQFALAPKISWLFTTLNKPKSKMKESIDDPSP
                     SPLKDSEFSSTEIRPARHRGPFNFAGRAE"
     gene            35806..36276
                     /locus_tag="VIG33_00775"
     CDS             35806..36276
                     /locus_tag="VIG33_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269393.1"
                     /translation="MQKIWHISKEGAQIKLRIPHPRHDVFLADLGYVSALLPETFEHL
                     NQLSIRFETVEAAFILDPHWKAALDEKKVTFEDIQFISKQASNVIQWIDLQLQVKKSL
                     WVETSFSPLTTAMRQQLQSQLQAHIARGDEKAAAIIRQFLDSPANAPLEEKSKI"
     gene            36273..36839
                     /locus_tag="VIG33_00780"
     CDS             36273..36839
                     /locus_tag="VIG33_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269394.1"
                     /translation="MNLLKLNLGCGFDKLPGYLNVDKEAACEPDQVVDLEATWPFETN
                     SVAEIRAAHVLEHLGQETKVFLGLMKEMYRVCVDDAKIYIQVPHHDHWTFHADPTHVR
                     KILPEGLKLFDQELNRQHIENKYANSALGIYCQVDFVLEKAVPSFDEPWASRIQNNLV
                     AQYDLNFAANHYTNAIVQWDIVLRVRKR"
     gene            36836..38413
                     /locus_tag="VIG33_00785"
     CDS             36836..38413
                     /locus_tag="VIG33_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269395.1"
                     /translation="MKEKMKVSNDKNFKLWLEAEKALSVGLPAGTAPEVLRTSGLLRA
                     QSQAWSAALLRLEEAVRNGISDLQTLDALGEAAYQTQTYQALLPFEDLYRDPMIAIHM
                     ARALMMLGQISAAHFFLRLAPDSLLKAAISAVLGVEKNIETSMTALFKPLIKKEMETV
                     NCIEYWQALAPVAEVAGRKDLVHLAEQRLKALAYARPVIHYNQSLRLLAEGEFRAGWK
                     LYDWRLVPGSACPAMTGFADFSMWEGEFLAGKKILVVMENGLGDQIFGLRYLQTLAEE
                     GAEVNVAVGPELAALAQSSFPSMKIHDLEKARELTYWKDKSRPDFWTYCLSIPARADL
                     GDPLATAGYLKASESLVLQCQRSLNLQNPRKLPVHGLVWHGDIRTAPMRTRAYTLTEF
                     LRESEILKTPCLLVCLQKDVTQEELSELRAQADKRGCLLFNAAPSLQNFAQTAAWINC
                     VDHMWSCDTSTAHLAGALGVRTTVMIRNKAIWHWRCEAKTQKAVWYDSCHIKYALTPP
                     ISYMFDIRDPSDSEGKV"
     gene            complement(38410..38931)
                     /gene="cysC"
                     /locus_tag="VIG33_00790"
     CDS             complement(38410..38931)
                     /gene="cysC"
                     /locus_tag="VIG33_00790"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEY8269396.1"
                     /translation="MGSCEIKSGVIWLTGLPAAGKSTVAQTLAKKLRSLGCEVEELDG
                     DAIREVFPSTGFSRMDRDQHVRRVGFMASRLEAHGVIVVASLISPFEDSRAFVRKLCK
                     NYFEVFVNTPLEVCEARDPKGLYKKARAGLIKSMTGIDDPYEIPPHPEMIIDTSALNP
                     EQAAEEILKSFLS"
     gene            39008..39916
                     /gene="cysD"
                     /locus_tag="VIG33_00795"
     CDS             39008..39916
                     /gene="cysD"
                     /locus_tag="VIG33_00795"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY8269397.1"
                     /translation="MKRTLDHHLDWLESQAIHILREVAAECARPVLLFSGGKDSAVLL
                     RIAEKAFRPGPFPFPLLHIDTGHNFPEVLRFRDQRAAELGEHLIVRSVEDSIERGTVR
                     LRNPLSDSRNAAQSITLLEAINEFQFGACIGGARRDEEKARAKERIFSFRDEFGHWDP
                     KKQRPELWDLYNTHIHPGENMRVFPISNWTELDVWQYIEREQLTLPTLYFAHPRQVIS
                     RKGWLVPHTELTPAQEGEPIETRMVRFRTVGDIPCTCPIASEAATVQAIVGEIALTHL
                     TERGATRMDDQTSESSMEKRKKTGYF"
     gene            39916..41214
                     /locus_tag="VIG33_00800"
     CDS             39916..41214
                     /locus_tag="VIG33_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY8269398.1"
                     /translation="MMTSVLRFVTAGSVDDGKSTLIGRLLFDSKGIFADQLEALTRAK
                     HQRTCGEDIDLSLLTDGLEAEREQGITIDVAYRYFSTPRRKFIIADAPGHEQYTRNMV
                     TGASTADAMILLIDAAKVKESLQQGQGLLLQTKRHSTIAQILQIRHIIIAINKMDLVN
                     FDQNVYEYIIKAYAEFAQSLGLSNIHTLPLSALLGDNVVVSSNHMPWYKGPSLIELLE
                     SLSLYDAKAKDQALRFPVQWVARHQGSETNDFRGYMGRIEAGKVRKGETLSVQPSGQS
                     ATVTEILSFNTSLESAESGQSVTLLLKEHLDISRGDMLVSAGHPPTLLKNVQADICWL
                     SERPLDLRHRYWLKHTSKQTLARVTDIQSCLDINTQQRHPTESLKLNDIARISLHLQQ
                     SISADAYDYSRATGAFILIDEVTHQTAAAGMIRLDTGAQN"
     gene            41223..42056
                     /locus_tag="VIG33_00805"
     CDS             41223..42056
                     /locus_tag="VIG33_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269399.1"
                     /translation="MNTMIKANKVDVVLRTCARTGVFNQKIVPRIVSEDRLTLIRTCI
                     KSLIVSINNSDKEITLTVFDDHSEPADLEKIKSILAECKVPTQVFTTQVPGFNNSALE
                     QFKYGRDHGRELVYFVEDDYLHDPQAIDLLVNAYNHFRNLSGFSEVAISPWDQPQEYD
                     VAHAQACKIFHFHNRYWRTTISTSNTVLWPLSAVKGGWHLFETLALQYGNVAGVEEIN
                     TIGQLLNNTVHHRGPVCCFSPIPSVALHIHHLGQGSPKYIDTQMFDWQKCWDKKKSDL
                     K"
     gene            complement(42095..42559)
                     /locus_tag="VIG33_00810"
     CDS             complement(42095..42559)
                     /locus_tag="VIG33_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269400.1"
                     /translation="MKINFKLALMAVLCTFSFKAYAIDVAPATTEDVKKFDSSLRAVS
                     EQENKDKDKDKDKDKDKDKTTKTKKDNFGQRTAAAAHKRNEERRELKRKELEEKKEDR
                     KQKDQDRDENREERRKDREDRREERRKDREERHEDRREDRPAAGGGDSDSRH"
     gene            complement(42556..43116)
                     /locus_tag="VIG33_00815"
     CDS             complement(42556..43116)
                     /locus_tag="VIG33_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269401.1"
                     /translation="MRDLKVQNILKQLDEDLVPDPYLSQKVWAEVSSKNSITKSYRRE
                     IFFWRGLSLASLGFLAVLLIRQHQPESVTSSAIPVHNPIVFHLENDSGIAYENVRMEM
                     ELPEGVEFHTNDARIAKKRTLSLSADRFEIGKSRLPFVVEAKSAGLKQFKIHVFDEND
                     KLIKEKILTIQFSEPQKAAFNQEKII"
     gene            complement(43146..43643)
                     /locus_tag="VIG33_00820"
     CDS             complement(43146..43643)
                     /locus_tag="VIG33_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY8269402.1"
                     /translation="MISKNEFQKIIEEHQQAVLRLAFFLTKNQAEAKDLTQDVFIKVW
                     EKWDSLGDVTRTLPWILKICRNQFLDNKRHRKYEDSFAKESLAESRHTLPASFEVWSC
                     LLKLEEEERLLLLLVDHQGLSYSETASVLKISEANAKSKIHRARQEFVKIWEEAATLP
                     KPRAS"
     gene            complement(43643..45205)
                     /locus_tag="VIG33_00825"
     CDS             complement(43643..45205)
                     /locus_tag="VIG33_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269403.1"
                     /translation="MDKTVMIFLIVFASLVECISFAQDSNEPAENIQFKEAIKDFQSG
                     HLIEARAGFLKLVELEPKRQIYWFNLCHTHYKLNNFQKASDCYKKVREWDGSLSLPAA
                     YYQAVCENNMGRSKKAVEIVTTVIEDSKCQENLRAKAVHFKKAVNDEEDEASVAALKY
                     FNEGDYEKSLKEIEKSDLSETPEAFLLRGMSYKSLHKNEEAKKYLQLTERYTQRPDIR
                     ESSRALAADLNEKEKPLLPGGFSGEILFDVGSDSNIYQVKSATASSITNFNVEVNYRR
                     DLSPQSYLIPGYSFKHSRYLKNSDLNTTDHFFELDLGYNSARWLFEVSPFFEVTQTTF
                     DLLSLGSDFLVKLDYDRWALGFSGTISEERSLATAENYMNGDLQTLKLFLELNNTAVM
                     VQLYMARLRTATGDMPLTVGTQTGILPLAYSGTESGLYTVIALRDLWKIYGRMAVTSK
                     NYDRISTPSNVKRNDLTTNLYLKIKYAFSSSWSGFASFNFFNNQSTLGTNDIENKNYA
                     ELISKFGLQWSW"
     gene            complement(45394..>47415)
                     /locus_tag="VIG33_00830"
     CDS             complement(45394..>47415)
                     /locus_tag="VIG33_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269404.1"
                     /translation="VTTAAPLDATKLPLAGGTMSGAIAMGGFDMTNAGNITMANNKYF
                     GLSSNATPSTVAGQMWYDSGTIKYYNGSSVQSLGVAGVGITSLNGLTSGTQTFAIGTS
                     GNSPAFSSATSTHTLNIPMASTASVTAGLISKTDYDAFNAKLSAVSDSASLTAGKVWV
                     GNASNAAVEQTLSGDATMTSGGVVTVDKTQSAVASKILQLTASSVAVTKGTDIGGAGS
                     GVAHIVYPNTATNTTLTLPSSAGSASQFLQTDGAGSLTWATALSSSTGFVNGGNTFAG
                     NSSIGNNDNYNLDIKTNNTSRMTILNNGNVGIGTTTPGSLLDVKGTLRLSGSTSGYVG
                     FAPAAAAGSTTYTLPTTQGSAGQFLTTDGVASTPTLSWATPSASLPSLASAKIWVGNA
                     SGAAAAVSLSGDIASVSDAGAVTLNTVPTTKGGTGLTSAGTANQILGMNNGATALEYK
                     TITAGSGVTVTHGANSVTIAATGSGGTVTSVSGTAPISVATGTTTPVISITAGSTTGQ
                     GLRWNGSAWAAGFIALTDLRSTVTGVNSFASSCSASQTLTYNSVGDVMSCANIAIANT
                     QVSGLGTLSTKSAAVLTTDVSGILPVANGGTGTSNGSITGTGALTFTAGGTNQDVTIT
                     ASGTGKTVISSAVVQLSGNSGAITSGVTTCAGLTGAITRDSNGDLYICK"
BASE COUNT        12394 a        11681 c        10732 g        12608 t
ORIGIN      
        1 ccgtggttaa ggacagtttg caagtcgcgt ttaatggctt cgcggattcg actttgaata
       61 ttttagtgaa ttttcatctg gccgtcttcg atgccaatga agaactcgct cgccagcaaa
      121 aaatcttttg cgaaatcatc gccacaggag tcggcacaga agtaagggct atttggagca
      181 aaagcccgct ttcgggtcct tttggcaaat ataagtcttc attgccttgt tctcggcctc
      241 gagcgcatta aatcgatttt cttgagtttt ttgccagttt agaaattttc catgcaattc
      301 tttgatcgcc tggataattg gaccgatgag atcccctgta gtcaacattt tggaacctcc
      361 aggcagcttt ttatcagcgg aattggtgag cacagcctga ggaaaaacct cttcaacctc
      421 ttgagcgatg aggccgatct tttccccttc tttccgatcc cgattttgat ctttccagtg
      481 aaagctgacg cccctgagtt taaggatctt ctccagcgcg ccatcaaggg gctgaatgtc
      541 gcgtttaagt cggcgatctg aaacattggt ccagccagca ttgccgccac agttggtttg
      601 cacacaccaa actccccaat taccgtaccc caaggctccg gaaccacccc cagcgctatt
      661 atagccttgt actgcaaaat tatttgagtt tgtcgtgctg ccctggacgc catgctgaga
      721 tccattgcca acaatcgccc aggtatttgt actattaacc tctagcttgg aattactagg
      781 ggaattcgtt ccaatgccga cgttgccaga ttgggttata gatataaata cattgctgtt
      841 atccttctcg attcggaagt ttcccaggta atccatctct atgccaacgt gagcggtacc
      901 tgcaagattg ttagagaact ctatcccata ggggcttgaa tttggagccg aagaggtggt
      961 ccttatataa agaccattag cacccgcact gccttttaaa tcaagaagcg acgtgggagc
     1021 cgtagttcca atgccgacat tgccgctgga ataataaata tccgccatat tcgcgggaga
     1081 gatttgccat agtgaatccg caagtcccag ggcataacaa ctattgagat tataggaggc
     1141 atccagagtc gcttgtaaaa aaatcgtcgt tgtcatttgc gggctgccaa tggtggagcc
     1201 gagcttattc attttgatgt tgagttcagc ttttcctacc ttatcgttaa cagggggatt
     1261 gtctgcaaca aaacccctga cttcaaaatc cgtgatagtg acgagattat taaggtaatt
     1321 cactccggtt tgaaatttta tattgggagg agtggcggca tccactattt gagttttagt
     1381 atatccggtg cctgctggat tatttccttt aaagcttgct gagcaaataa ccggatctat
     1441 taaaagctga cggatactga cggtcagttc tcggtgggca tctttagact gagcggtgcg
     1501 ctgggacttg aacacgtctg tgatcattgt cgtgatccca gctaaaacaa tggcgcctat
     1561 accaactgcc ataagaacag tgaccaggct cgtacctcgg ggatttttaa ttaatttcat
     1621 gcttaaatat cgacagaaag aagaagatac ttaacgtaaa cataccataa tttgtgtgcg
     1681 agcgaattaa tgggctacat tagctagtaa atacgtatga atttactcat ctaataaagc
     1741 ctccatagct ttaaaaagag aattgcaacc tttctacaaa aaaaatataa agagatttga
     1801 cattttttcc gatatcttag gatatgaaag cctctcctcg tggtacgtta atcacacctc
     1861 caagaaatgc atttttaatt acaatgcatc tgattttatt gctgtcttca gtattcttaa
     1921 gtgttaagcc agctctggct gcggcttcag gctttaccta tacaggtaaa atcttagact
     1981 ccaataatgc tccggtgacg gccacctcag tgatctttac cgtcacggtc tttgatcctg
     2041 caggaaaatg ctggctctac actgagcaaa gaaaattgga tttatcgcaa accgcgggga
     2101 ctttttcttt tgaaattggc tcaagcgaca ccgcgactct ttatggcgcg actccttctt
     2161 ttaataacca atccagtggc ggacctaaag acttagccga tctttttaat aataaaaaat
     2221 cctatgtagg tctgggggct agcaatggct gtactggcac ttatgatccc agcacttctt
     2281 cagatcccaa tgaaggtcgt ttgctttcag tttattttag agtgggaagt tcaggcacgg
     2341 atcaagccat ccctccgcta agaatcaccc ctgttcccat tgccatgcaa tctttagcag
     2401 taaatggtta tggtacgggt gagcttttaa gagtggatca agcaaccgtg gcttctgcgg
     2461 ggatttctaa taatgccctc tcggtggctc agtacacaga gttctgggat ttaatgaata
     2521 aagccagcat cagttatctg ccggtaacag gagatgtgtc tttagtgggg ggcaataata
     2581 aactcaccac tttcttaggc caagccttac cttcaggtcc tgcaagcaat ggtcaggtgc
     2641 tggtgtctaa tggcacagcc tgggtcttac aatctatgag cggaggtagc ggcacagtga
     2701 ccagtgtgac tggcacggct ccggtggtgg tcggaggcac agcttcagct ccggttgtaa
     2761 gcttgccggt agcgacgact tcagcaagcg gttacttaac ttctacggat tggaatactt
     2821 ttaacttaaa actttcctca agcttagctt caggaaaaat ctttatcggc aatgcttcag
     2881 gtcttgccag tgaagtcagt gtttccggcg acgctacttt agataatgcc ggagctttaa
     2941 ctttaaaaaa caccggcacc gctggcactt atggttcagc aagtttagtc cctattgtga
     3001 cgacggatgc tcaaggccgg gtgacaagtg tcacgaccgc agccccctta gatgccacca
     3061 agctcccgct tgctggcggc accatgagtg gcgcgattaa tatgggagcg caagatatta
     3121 ctaatagcgg gaatatcacc atggcgtcga gtaagacttt aggactttcc agcaatgctg
     3181 cggatcctgg aagtcccagt gccggtcaag tttggtataa ctcctctgta aatttgatta
     3241 agttttatga tggcagtagt gtgcagtcct tgggagtcgc tggggctgga attacaagtt
     3301 taaatggcct caccgcagga ggacagactt ttgccattgg cacttctggc aactctccgg
     3361 ctttttcttc agccaccagc acgcatactt taaatatccc catggcttca agcgcttctg
     3421 taactgcggg tctgattagt aaagccgagt atgatgcttt tactgcaaaa cttggagctg
     3481 taaccaatac ggcaacgcta gcgccgacaa aaatctggat aggagacgct ggcagtaagg
     3541 ctcaagagtt tgccctgtca ggcgatgcca caatgacttc cggtggcgtg gtgagtgtgg
     3601 ataaaacgca aagtgcggca gccagtaaaa tcctgcaact cactgcaagt tctgtggccg
     3661 tgactaaagg aactgatatt ggtggagcca gtactggaaa agtttctctt agatacccaa
     3721 gtacagccac tgacaccact ttcactttcc caaattccgc gggttctgca aatcagtttt
     3781 tacaaactga tggaagcgga agcttaagct ggggaactcc ggcggcgact ttgccagctc
     3841 ttgcttcaac ccaagtgtgg gtggggaatg cttccggagc cgcagcggca gtggctctct
     3901 caggcgatat ttcaagtatg gataatgccg gagtggtcac cgccaataaa accacgacag
     3961 cgcaagcgag taaattactt tctttagatg ggagtggtgt tggagccatg atggggaatc
     4021 agttaaatgg tttaacctcg ggttcgttga ctttgcaacc tgcggctata acaacaaatt
     4081 actctctgac tttcccagcc gcgcaaggag cagcagggca gactctgtca aacaatggct
     4141 ctggagtttt aagctgggct tcgactttga ccaactcttt aacttcagca aagattttag
     4201 tcggaaatgc ttcaggtctt gccacagaag tcagtgtttc cggcgatgct acaatggata
     4261 atgccggagt ggtcaccgcc aataaaacca cgacagcgca agccaataaa ttactttctt
     4321 tagatggcag tggtgtggct tcctcaatgg ggaatcagtt aaacggttct acttctggtt
     4381 ctctgacttt gcaacctgcg gctataacaa caaattactc tctgactttc cctgccgcgc
     4441 aaggagcagc agggcagact ctgtcaaaca atggctctgg agtcttaagc tggatgaccc
     4501 cattgagttc aagcacaagt tttgtaaacg gtggcaacac ttttgcgggc aactcaagcc
     4561 ttggaaataa tgacaattac aatttggata tcaaaaccaa taatctcagc cgcatgacta
     4621 ttcttaatag cggaaacatc gggattggga cgacatcgcc agcaacaacc cttgatatca
     4681 atggtagcct aaaggtggga tcggatccaa tttgttctgc agataaggcc ggagccatgc
     4741 actggacagg aagcaccttt gagggatgca acggcacatc ttggagtgca attggagcga
     4801 tgggaacacc cgctcaaaat gtagtgatct ccttcagcgg tggtaacaat cagactgtga
     4861 ctttggccaa caatagtcca attaccaatg gcgatgcgag agctgcatgg tcggtctaca
     4921 cgaatctgcc taattacctg ctcggaacat tgggtacgga caaaattaat ggcagtaatg
     4981 ttacattaaa tctaagtgcg gcaactacct gctacctgtt gcacgatccg ggttgggttg
     5041 ccgtagactt gacgggctgg actcttcttg aatcaggaat gccttatgtc tctacttcaa
     5101 ataatatcaa cgtctattcc aaaaatctgg gagccggcgc ttacactttt tctgtttaca
     5161 gtgcaatata ttcatgtgtc ggcacagcct ataattgggc tatgaatggg agttcccttt
     5221 attattctgg cggcaacgtc ggcattggga cgacaacgcc aacggcgaca cttgatgtga
     5281 atggggaggt caaatttgga aacacctcct cgatttgcaa cgcctcaaaa gaaggacagc
     5341 aaaggtacaa ttcaatatcc aaaataatgg aatactgcaa cggcacagcc tggtccccgt
     5401 ttggatttac aacaactaca accgttagct gcaccgtcgc tgtagccgga ggatggaatg
     5461 gctgtaccgc aacttgtgct gccggatata atcgaacagg ctgttcctca tcgaatgata
     5521 gtgcttggag cggctatatt gaccgcggag cagttccaag tggttccaac gcttgcaatt
     5581 gcaactactg gattaataac ggttctaccg ttagcttgga atgcacagcc tattgcgcca
     5641 agtaagagtg gattcaatag aatccgaaga aaaataccaa gagaaacaga ggaaacttgg
     5701 atcctatttt tgtgcaaaat atccttcaag cctatttcac cggaacctga gggaagtatt
     5761 gagcgaacag ccagaggcgg aactcttcga tcaccttctc tgaggtcgta ggcgccactt
     5821 cggcacggaa tttatctttt ttctgatctt ccgcgagaaa gcaattgaac tcaaaagttt
     5881 cgtcagaggt ctttaataag accgtaaaat aaccagcgac ccaatccggc aacttgtcgg
     5941 taaaacaaaa gccaccctcg gaaagatcca ccgtgtgtgt gggaaaactt tgcgatccca
     6001 ccacgatttc cacaggaatt gtggctacaa aacggatgta cttacgcatg atcggttcac
     6061 gcagaggagg agctggtgat gccgaacgaa ccacagtgat ttcatgatcg ttttcttgag
     6121 tgatttgcgg caaggccggc aaagtcacta aatcactatc ttgaaacaaa aagagcacat
     6181 gacagtcttc actgccgaga ggcttccaat tctcccactg tttctggtga gcccagctca
     6241 ccttcatttc actatccgtc attgacgcca ctaaaagctg gcattccctc aggctgaggc
     6301 cagagagagt ccaggactta agtggattat gaagcgccca acgacgggac gaaaaaaatg
     6361 aaaacatata tttttcttac ttaatgcctg cggtttcagc tctgattttc gctgtttcca
     6421 acatcatctt ttctgtttct gtctctgctt ttgccgtatc gacacggata gcgcgctcat
     6481 ccgcctttga ctttgccttt tccagagctt cttcgatgga ttcacgctct tttttggctt
     6541 tgtccgcctc agcctttaag cgggctaatt cagcatcggc tttggcggat tgcgcatcaa
     6601 actcggcaat agcttttctg gatttattaa cttcgaaggt ggactccgca gtcacctttt
     6661 gtaattccgc ttttttagcg tccaactcag ctttggcttt cgcataggat tggcgggatt
     6721 tttcttcatt gctcttagct tctgccaatt tctttttctc aacctctaaa gcgtctttgg
     6781 ttttagcaag ggcctcttgc gattgcttag aatcttcatc aagttttttc acggtgcctt
     6841 cagcctgtgt ctgtgcggct tgagcttttt ttatttcagc ctcagcacgg gatttttccg
     6901 catccaggcg ggccatctcg acttttaccc gagcttccgt cgcttttcct tgagccaccg
     6961 ccttttgcga attcaactgg gtttttgcca actcctgttc agtttgcttc aaacgcattt
     7021 tggcttcgtc cagttctttt tgcgatttct gagcgtcttc catcgctttt tggctttctt
     7081 tttcaaaatt cttcatttta cccgccactt cacctgaaga cttttcagcg atttttctct
     7141 tttcgatggc ttgttctttt aatgtttttg cttgaatcac tttttcttga gccgctttga
     7201 tgcgggcttc aaattgctgc tgttgcagtt ccgcctgctt ctgctgtttt tcattttcgg
     7261 ccgtctcttg tttgaggcga gcgacttcac gctccgcttc gacttgttct tttttagcat
     7321 tggcttcgac ggcgcgagcc ttttctaagg catttttggc ttcgctgcga gctttctcca
     7381 gtctttttct ttcttcctca gctcttctct tggcctcagt ggcatccgct ttcgcactct
     7441 cagcatcggt tattgcctct tccgcatcca cttgggtgga atcaaccgat ttggcatggg
     7501 ccaagttcaa caaaacacat agtaacaaca gtggtttcaa aggcgtgcac atgagaattt
     7561 cctcctaata ttaaatctta cactaggagg tatcggttga agttagggat gaattaactg
     7621 gcctttgggc gcgtctttat tcacagactt tgattttgcc cgcggcctta atcgctaaat
     7681 tcttggcttt ttcaatattc gcactgcgag cgagcgccac ggccatgcgc cggttgggtc
     7741 gagtgctggg ctttccaaaa atacgcacgt ccacattttt cagttccagg gctttttcca
     7801 caccactgaa ttggggaggc tttgccgact ctttggtggc caaaacaacc gccgaagcac
     7861 tcggtccata atatttgata tcaggaatgg gcaaacccaa gatcgcacgg atatgcaaat
     7921 caaattccga aagatcctga gagatcaaag tgaccatacc cgtgtcatgg ggccgcggag
     7981 aaagttctga gaagtacaca tcctctttgg taataaagaa ttcgacgcca aagatgcccg
     8041 cgccccccag gcggtcggtg actttcttgg ccatcttttg cgctttcttt aaaagagctg
     8101 gtttcatttt tgccggcatc caggattcac ggtaatcacc atgctcctgg cgatgaccga
     8161 tgggatcgac gaagagggtt ttgccgtttc tttgtttgac cgtgagcaaa gtgatttcga
     8221 aatcaaagga aatgaaagac tctgcaatca ccttcttttt atcacctcgc atcccttcgc
     8281 aggcatagtt ccaagccgcg cccagttgct ttggtgtttt agcgacggat tgtccttttc
     8341 ccgaagaaga catcactggc ttcaccacac aaggaaagcc cactttgtcc gctgcggcca
     8401 ggagttcttc cgggctcgaa gcataagcat agttcgctgt tttcagtttc aattctttgg
     8461 cggccacatc acggatggca tcacgattca tggttaagtg agcggcttcg gcggtgggaa
     8521 taacgaccac tccttttttc tcaagctcga tcaacttttc agtgcgaatc gcttcgattt
     8581 ccggaacgat gatgtcagga cgatgctttt taacgatgcg ctcgatctca gctccatcca
     8641 gcatgtcgat cacttcgaaa gcatcggcca cttgcattgc gggagcccct gtatagcgat
     8701 ccaccgcgac cacgaactgc cccaggcgct tcgcagagat cacaaactct tttcccaatt
     8761 cgccggcccc taagagcatt atttttttca tgagattctc ctgttaactt tgaaactaac
     8821 tttcattcaa acaactaaat tagaaatgaa tttgatctaa taaaacggct cgcactggcg
     8881 aggcctctgc gccgtgaatt tttaaaggca gcgctatcag cacataaagt cccggtgaca
     8941 ccgcttgcaa aaccaagcct tcaaggatgg aaatgtgatt tttgaaaaac gcctgatgag
     9001 tttcaagact cttactctga gaatgatcca tggagggcgt atcaatgcca atcaacttta
     9061 cctgttgagc cgccaaccaa tcgatcagtt ctggagataa ataactaaaa tcttcctgcc
     9121 aaacattcac gtcgagtatg gaattggttt taaacaggat ccgcggcgct aaaatatcca
     9181 ctagacccaa tttttccaat gtcagctcac ccttcaggct tgaggcatca atgacctgac
     9241 agggaccgac ataggtctct aaagagcatt ggtcgatgct cactccctct ttatgataat
     9301 gtgagggggc atcggcatgg gcgcccaaat gcaaggtgga ctctatggaa ctaagactga
     9361 gaggatcccc ctgcgcaaag tccatggaga cccttctctg aaaggatcta tccccaggaa
     9421 aaacagcaag gctcgacgta atcagcggag aaatgtcata gatcaacgaa gcaactcctc
     9481 cactcgaggt ttgatttttt ccataaagac tctcatgctc tttttaattt gttcgttatc
     9541 gtgattatta aagtcaaacc acaacatcaa acgatcctca ggatgatact ttttgacaat
     9601 ggcctgagcc acctcttctg gattgcccgc gatggaattt tcaacggcgc tttccacttt
     9661 tttcggatcc aaggtttttt ccatggccac ccaatagctt tgccaggctt tgcgggattg
     9721 ttcgcgggcc cgcgcggatt gttgctgcgg agacaatcct tccgtggcat ccacaaagat
     9781 cagggctgtg cgcggcatat gcgcccgcgt ccaggctccc ccatcggaat gaaattcttt
     9841 acgcatacga caatgggttt cttcaatcac ttccgcggga gtgattgaaa gattaaacac
     9901 tccggtcggc agaatctcgt tggccattcg ttgcgttgtt ggatcatggg acccgatggt
     9961 caaacgcaac aattccaacg gcgcttcaaa aggaataacg cccactttat caaagacaaa
    10021 gaatggcaaa tgcagaatct gctctgcttg cccgattttt ccgccatccc aacgacggct
    10081 gtcctcttta gctaatttat aaaccttatc ccaggtggct tgatcacgga aatcttcccg
    10141 acgcaaatag agagggccga tatctttgct ggcaaaatga tcaccgcgca aaagtcgcaa
    10201 catgatcaaa gtcgcttctt gaaagacttt attttttaca acctcccagg cggcttcatc
    10261 caccgggtcg cgcgccttaa taccataggc gtgataagaa aaaggaaagc ggcccgcggc
    10321 aaaacccaat tcaagaaccc gcttttcatt cggcctgaga gcatgcagcg aaagaaaagt
    10381 cttcacggct tcagcatggg ccacagggcc cccattgcag aggatattgc gaatcgctga
    10441 accgacattt aaattcttcg tctgctgaaa gatcagatgg gccagctgaa gaatatccgt
    10501 attgagtccg atttccccct tgaaatgggg aatcacggct ccaggatttt ctttttgcac
    10561 ttggcaagac aaatgtgtct ctgccagcca ggccgtgccg aaaccaaggg tatccgcgca
    10621 acggacttgg tcaaaaaagt tttcccacat ggttctctca ttggggagat aaccatcaac
    10681 ctctgtctga cagatcgaaa agaagatatc gtatttcatg gcgaacatta gaaacaaaag
    10741 aactttaaat gtcagtcttt tctcactgcg ctattaaaaa tagtctaaat atcatactaa
    10801 taaattctct cgatttaggc ataaaaatat ggttgcattg ctctgtttaa gaacatgccg
    10861 aggaggattt atggtgttgg atttggccaa tagtttggct aataaaagag ccttgatctg
    10921 tggcgcctct caaggaattg gcgaagccac agctcagacg cttgcaaaac tgggcgctga
    10981 agttattctg gtggcacgca ctcaagaaaa actcgaggcc gtcaaatctc aactcgccac
    11041 aaagggggga caaaaacatc aggttcttgc tctcgatctg tctttgacag actccctgaa
    11101 ccacgccctc gactcccttt tggcgacggg ccctatcagt attgtggtca ataatgcggg
    11161 cggtcccaaa ggaggagctc ttctggaggc aacggatgag gaatttctgc agggttttca
    11221 ggctcatatt ctggcctctt ctctgatcgc aaaaaaagtt gttcccggaa tgaaagaaca
    11281 gcgttgggga cgcttcatca atattatttc cacttcggta aaggcgccga tcgccaacct
    11341 gggagtctcc aataccattc gtggagccat ggccagttgg tcaaaatcac tggccaatga
    11401 gttggctcct tttggtatta ccgtcaataa tgtattgcca gggtatacgg aaactccgcg
    11461 tttggaggcg cttgcccaag cggcagcgca acgacaaggc aagagtgtgg aagaaattaa
    11521 aaatctttgg aaggccgcga ctcccgctgg acgctttgcc aaacctcaag aggtggcgga
    11581 gaccatcgcc tttttagcta gctcttccgc aggctttatc actgggatta atcttcctgt
    11641 ggatggagga cgcacgccat gtctttaagg gaaatcaaaa actttatctg tggcgaatat
    11701 tccgccgccc aatccaaaaa gcgcatcgac aatttcaatc cgtccaatgg gaaagtttat
    11761 tccacccttc ctcgatcaga tgccggcgac atcgaggccg ccgtgcaagc tgcccaaaaa
    11821 gcctttcccg cctggtcacg cacaagccct gaagagcgtt cacaaattct gcttaaaatt
    11881 gccgaaggaa ttgagcgtcg tattccacag ttggccgagg cggaatccac agatcagggg
    11941 aaaccggttt ggttggccga gactctggat atccctcgtg cggcccacaa ctttcgtttt
    12001 tttgccggtc aaatcttgtt tccatgtaac gaatcccatg aattcaatga aaaagctttt
    12061 aactttagct tgcgcaaacc agtgggagtg gcgggtttaa tttcgccttg gaatctaccc
    12121 ttgtatttat taacctggaa gattgcgccc gcgctggcct gtggcaacac cgtggtttgt
    12181 aagccctcgg aaatcactcc gatgacggcg tttttattct gcgatattct caacgaagcg
    12241 ggacttcctc ctggcgttgt gaatatggtc tttggtttgg gagtggaagc tggcgaagct
    12301 ttagtcaaac atccgaaagt tccgctgatc agttttaccg gcggcaccac cactggaaaa
    12361 catttgagtt ccgctgccgc tcctttgatt aaaaaactct ccctcgaact cggcggcaaa
    12421 aatgccaata ttatttttgc cgatgcggat ctagaggagg cgattcagac ttccctgcgt
    12481 tccagtttct tgaaccaagg tgagatctgt ctttgcggct cacggatttt tgtggaagcg
    12541 tcgatttatg aaagcttcgt ctcccgtttt gtcagtgaaa ctaaaaaact caaagtgggc
    12601 gatcctcgca aggctgaaaa ctttctgggc gcccttgtca gtaaagcgca tctggaaaaa
    12661 gttcagtcct atgtgaagct ggcccaagac gaaggcggca agattgaaac tggcttcgag
    12721 gatattccct taaccgcaga acagcagcag ggctattaca tgcgccccac catcatcacc
    12781 ggtttgcgct ctgagtgccg cgtgatgcaa gaggaaatct ttggccctgt ggtgaccgtg
    12841 gttccttttg gttcagaaga ggaagtcgtt tccatggcga actctgtgga atacggcttg
    12901 gcttcgaccc tttggaccaa agacattcag cgggtgcatc gcttggcccg acggctcgag
    12961 gtgggaaccc tgtgggtgaa cacctggatg atgcgcgatt tgcgcacccc ttttggcggc
    13021 atgaaaatgt caggactcgg acgtgaaggg ggcttgcact ctttggagtt cttcactgaa
    13081 accacgaatg tttgtattaa atattagaaa tataaaaagg aaaatctatg gcaacaaaaa
    13141 atcaaggaaa gattcaagga agcagtcgag gaaagattgt ggcaggctgc ttggctcccc
    13201 acccacctca tcttgtttat gcggaaaatc ctccgcaaaa tgaaccgcgc tctgaatgcg
    13261 gttgggaaac tctgcgctgg gcctatgagg ccatgcgcga agatcttaaa aagattgagc
    13321 atgatgtttt ggtcatcctc tctccgcact ggcgaactta tgtgggaact catatcttgg
    13381 ccactcccca tttgaaatcc ctttccgtgg atccgatctt tccgaatttg tttcgttatc
    13441 actacgacat caaggtggac gtggatctgg ccgaaaccat ccacgaggtt tgccaagcca
    13501 gaaacatccc caccaagctg atgcagaatc ctgatttccg cgtggattac ggcaccatcg
    13561 tgaccggaca aatggtcaac cctgcttggg ataaacccat cgtctgcctt tcggcgaact
    13621 gtgtggcggc ttactataat ctcgatcgca tgcaagccct gatgatggaa ttgggagcgg
    13681 ccacgaaaga agcgattgaa aagagcggac gtaaggccat cttgttggcc agtcactcgc
    13741 tttcgcaccg gcattttacc gtggaaaatg aaattcccga agatatgagc aaagaacata
    13801 tcaccaatca cagcctctat ctttgggata tgaaaatgct caaattgatg aaggaaggaa
    13861 aatccaaaga aatggtggag ctgatccctg acttcaccga acaaacgatt gccgaatctg
    13921 actctggggc tttgacctgg atgatgaagg ccatggactt tccgacttat cccgcgaagg
    13981 tgcatggcta tggaaccgtg atcggaaccg gcaatgccat cgtgcactgg gcgcctccct
    14041 tgcaagcccc ctcttctact gagaaaggac tttaatgatg actcaacata aatctaacaa
    14101 tcccgtgctg aaaggattcg tcttgccagg gcttgtccaa cccctgctta ctcccgatgc
    14161 gaaccccggt tatcgcaagg tgcgcgccgc cttcgataaa gtgcgggaag agattgagca
    14221 cagcgatgcc gatgtgcttt taatttatag cacgatgtgg ccctcaatct taggtcatca
    14281 aattcaagcg atgccagagc ctgaatgggt tttggtcgat gaagagtttc atgaactcgg
    14341 aacgattcct tataaactta aaatcgatag cagctttgct gagtgctaca accgtcacgc
    14401 caaactgcgc ggactgcaca gtcggaccat tgcctatcat ggcttcccga tcgatagcgg
    14461 ctcagtggtg gcattgaaat tgatcaatcc caacaatcga attccagcgg tcattgtttc
    14521 gagtaatatt tatgccgacc gtgccgaaac tgtggtgttg gcaaaggctg cggtttccgc
    14581 tttgaaagaa caaggcaagc gcgccattgc ggtgattatt tccacgctgt cgaatcgctt
    14641 gcattcggat tttattgatc ctaaagacga caagatccac tctttgaaag atcaagaatg
    14701 gaatttaaaa attctggagt ttttagaaaa aggtcgcctt gaagatgtcg cgcaattgtc
    14761 gcgacaaatt cagcgggaag cccgcgtgaa aaaggtcact aacttcaaac ctttctggtg
    14821 gttgtcttcg gtcatgggct cccacaaccg ctatacggga cagatttacg aatatcaacc
    14881 gatctatggc acgggctgcg cggtggtggg tttgactccc gccttgcagg cctcccgcga
    14941 tttggaattc gacgaagatg ccccggacta ctacaccggc gagcgcaatg ttttaagcgc
    15001 cagcggcgaa tccgcagcgg atttctctgc catgtctaat ttatacaata acctcgatgg
    15061 cgaaggccaa acagaggagg acgatcatga aaatttctga tgtttatcca aagagtccct
    15121 ttggtgaaaa aatccccgga attcccaccg ggcgtgatgt cgagtggcag cctctcatcg
    15181 actatcgtcg caatggcgtc agtgaaaaca cgattcacgg ggccatcgcc tggtacagtg
    15241 gcgatgagtt gatccactca ttcggcggca acgttctttg ttatggccgc agcatgatga
    15301 aacttttcta catgaaagtt ttcgcccagg aactcgccaa ccagacaagc tgggagcaaa
    15361 aggccatcag cgtttccagt cataatggag cggcagagca tatcgaagcg gcgcagtcgt
    15421 tgttggcgga gagcgaatgg ggtctgatgc agactccgct ggatttgcct ttggttcaat
    15481 ttgggcgaca ggtgcgacgg cctcgtcgct ggtacaataa ctcctcaggt caccatgcgg
    15541 cgatcttgtt gggttgccgc ttaaagggtt ggaatcgcgc gggttacact ctgcccaacc
    15601 acgaagttta tggggcttat cttaaagttg tgcagcagta tctaggaaaa acttggtctc
    15661 ctcttcgtgt ggctcgtgat ggagatggtc ttccgaccgt cgcgatgacg gtgaatgagc
    15721 tggcgaaatg ctatgccggc ctatcaaaga atcgtcatga agactggatt tgggagagca
    15781 tgatccgaca tcctgattta gtcggcggct ttaatcgttt ggatacgact attatgaaat
    15841 cttgcaaagg ggatgtaatt gcaaaagagg gcgccgatgg tttgctcgga atggcgatct
    15901 ctcaccctga gtatccgcaa ggtttgggcg tggtgatcaa gattgcccat ggctggaatc
    15961 cacaagccac gtggtatatc gccagaggca ttctggggtc tctcggtttt gagcttcgca
    16021 atccttatcc tctgcgacgc cagaaggctt tcttggttcc acaaatcgct ccagagaagt
    16081 atttctcacg tctggaaagc attccaactt gggacgaatg ggatccggat catgatcgct
    16141 ggtacatgga agaaagcatg ggagaggata agggaggctt caatggctga tataatcaac
    16201 gccgccaaag ctccagaacc ggtaggcgct tatccccacg cacgtcgctt cggcgacctg
    16261 ctctttcttt ccggagtggg accccgcaag cccggcacca aaaaaattcc cggagtggaa
    16321 ctcgatgccc agggcaatat cacgtcctac gacgtggttg tgcaaacgga atccgtgatc
    16381 gagaatattc gcatcatctt ggaggcctca ggaagttcgt tggaaaagat catcgatgtt
    16441 caagtcttcc tcacgaacat gaaggccgac ttcaaaagct tcaatgaggt ctatgccaaa
    16501 cactttacgg cgacaggcgc gacccgcacc acggttgaag tgggagccct acccacgccg
    16561 atcgcggtgg agttcaaggt cattgcggca ttctgagggt acgccgtacc tttttcggat
    16621 gcaacgcatc cgaaaaaggt acggcgtacc ctaatggcgg tgatggatat ctggaaagtg
    16681 cgaatgctga tgccgcattt tttcgtgctg gtgataatgc gtgtgtttct tgccgaagct
    16741 ctctgccggg agatgctcgt gctgatgatg ttcatcatca tgagtgtgct cgtgcgaatg
    16801 gtacaagggc tcgtgctcgt gttcatgatt atgaatctcc gtcacgtgca gccagaggcc
    16861 ccagcccata aagcccgccg ctaaaataaa ttgcagcgag agttcgtttt taaagagaaa
    16921 taaagaaaga gtcgccccca caaaaggagc tgcggaaaaa taagcgcccg tccttgaggt
    16981 gccgatggct cttaaagaaa ggacgaagca tacaatactc aaaccataac ccaagaagcc
    17041 cagcagagct ccccacgcca gcgggcccgc tgccggaagg ctccgcccca gggaaagggc
    17101 taatataaga ttcgtcgagc ccgccacgac gctttttaaa gtggtgatta agaccgcatc
    17161 attagccgac accatgcggg tcaaattgtt gtcgatcccc caacacaggc aagcgcccag
    17221 aatgaaaaaa gacccggaac tcaacgagaa ttcccccgct gaagaccaag ataaaatcat
    17281 tcctcccata ataatggcca tcattcctaa gacaatccgt ctgtcataat gttctttaaa
    17341 tacgatccac gcgatacagg aagttaaaac gccctccata ttcaacagca gcgaagacgt
    17401 cgatgcggtg gtcaagctca ggcccttcat taagaaaaca ggaccgagaa ttcctccgaa
    17461 aaaagtggct cccatcaacc aaaaaaaatc ctttttattg gggaaaaccg ggagcgattt
    17521 ggccttaaga gattgaacag ccgagatagc taagaggcca atgcctgatc caaaataaag
    17581 gattcccgct agaatccatg aatccatctc gccgattaat actttagcta agggggtgct
    17641 ggctccgaaa agcgttgctg cgaatagagc cggcattatc tgatggatca tttttaattc
    17701 tttaaacata tattacacat ctcaaacccg agtggcataa tatgcaactt aatagcatta
    17761 tacgctactc agtgttaggt cgtgcaaacc cctcggctcc attttagttc agattgtagt
    17821 tagcattcaa gacctcattc aagctcgtat ctaaagtgag tggcaggcca gcgatgccaa
    17881 acttaacggg ggcttgcaag aagaccagac ctccatgatt tggattgacg atgatgccgc
    17941 gggttttcga gcgatgcaat ctgggttcaa aaggcagctc ggaattttta tcagagcttt
    18001 taccgcgatc attgggatta tttgcaactg accactgatt cgggacctcc gtggttgtcc
    18061 aggacttgta gtacatggac atccttgccc cctcgataag ataattgatc tcaaccccgg
    18121 gacccgccag ctccagaaca gtgcggaagg tgataaagtt tttcgccatg gcttccccaa
    18181 attcgccata ggctctcgca aaggcttttg cgtcccgatt tttttccgtg tacatttcta
    18241 ataaggcccg atacattttc tcaatcgcat tatagctgtc atcgcgaaga tccagccaac
    18301 attgtggatg aatgcctttg caataaccat aaacatcttt gctcagcgaa taactctgaa
    18361 gatatcgatc cgcgacttta tatatggctc gttttccttc gcgttgcgcg aggtccatca
    18421 aatgcagagt gtgctctttg ctcaaggtgg tattaaaatc aatcgcggcg taagccagat
    18481 tctcctgagg aatatcgact tgcagctcat ccatggccgt caaattaatc aacttacgaa
    18541 cggcgccgtt gagtttttca ccactactat ggtcattcat ataagcatac ccataacgtc
    18601 caaacattcc cacgtccgtt ttaccttgtt cctcaatggt ataatgagaa ccatagaatc
    18661 tgacgtctgt ttcacgctgc ctattgaaaa aacggtaatt acttttttct gaataaatac
    18721 cataattcac tttggcgact ttatttttga catgaaagtc cgtcgcggag tacgacaaaa
    18781 ccttgccacg actgtaagtt ttattccaaa tcaggggaag ccccaactcc gcactgataa
    18841 gtcggcctgt cgataaattc ttaaacgtgg aaaccttcaa taccggcgca gcttcaacat
    18901 agtttgtcgg aacggcttcg gcaattttct ctgaagcgat tatatttccg cgaatcgcat
    18961 cttcataagc cttacgtccg acttcagtat tgagattgta aagaaaggag aacccatcat
    19021 ctctgctttt aaaacccgca acagaaagct caacaatggt tgccgcggta aaggcggaca
    19081 aagtcgccat ttttcctttg gtgatcttta aatagatttt attttcttcg atcttctcaa
    19141 tgtaggtttc ccaagtccct tgagccacca gtgcggcccc ggcatccacc agactgtatc
    19201 cgatagttgc gccgaataac aagccgccgc gacagatata ggtgatgcta tcgccctgac
    19261 tccaagaaat taaatcactc gcatgcccag gaacttttaa ataacccttg ccatgcctgg
    19321 cctctgcgac agtcttgaaa tacctgacgg actccacatt cactccgaca atgggcataa
    19381 tccctacata agaccaccat cgggtcagga gatcagagaa acttctatac cagaaggcca
    19441 cctttgcccc taagcccaga gaaagagtgc cgtgggtcag cttttggaga ccgtcgcgat
    19501 agtcatccat gatcatgcgg tcacgacgaa atttagataa agattcagat tcagataaag
    19561 aagacggctt cggattatag ggagtcagat ttacagtcca agttccccag tcaaaatcca
    19621 gaaaaaagaa aggttccacc cgcggaggtt tcgagcggaa aaaatgctcc cctccttcag
    19681 caaaactcgc tctagagaaa tttattaaaa gaacaaaaga caaagcaaaa atacgaaaaa
    19741 gcctcgaaaa gccagccata tattctcctg catgtttata tttacaactt aaagtttatt
    19801 gttaaacaaa aacatgcaag tgatatgatt actcactgtt aggaagcagt ttttaattag
    19861 tgatgaaggt aattcaagat ctgagaaatc agcagacttc gatttatggg cttggtcaaa
    19921 aaatcggaca ttcccacgtt caaacaatgc tctcgctcct ctttcatagc atgcgccgtt
    19981 aaggcgatga tgggccgctg atatccttgc tgtcgaagtt tcgtggtggc ttcataccca
    20041 tccatttcgg gcatttggat atccatgagg acaatatcaa aattcttggt caaagctttt
    20101 tccaacccct caattccatt gtttgcaaaa tcaatcttcg cacccgccac agttaagaaa
    20161 cgactgatca gagtttgatt gatgggagaa tcttcgacca acagcacgct tattccctct
    20221 aaaagtcgag cttcctcagc ctcattcata atctgcagac ttcgcgggag caccatctca
    20281 atatccgcaa taaaaagggt gttttccacc gctgccgctt tgacctcaac cacaaaaaca
    20341 ctcccgcgtc ccacttcaga gctctcaaga taaacatctc cgcccaaaga tcggccaatc
    20401 gttcgagcga ggaccaggcc caggcccgta ccgccaaagc gtctcgatat ggaattgttc
    20461 acctggctaa aaggttgaaa gagtttatcg atttcatccg cttctatccc cactcccgta
    20521 tccagaactc gaaacttgag cctgccctct atccaatagg cctccactgt gacttgaccc
    20581 ttttccgtga acttgatggc gttactaatg atgtttaata aaatttgccg aagacggatc
    20641 ggatcacaaa aaatatgctc agggatcaat gacttgaaac agaaattgag agggattccc
    20701 ttttcttccg ctttgatcct catcagggcc caaagatctt ttaataaatg ccttagattg
    20761 acatccagga tttcgatctc taaatgtccc gcctctactt tagagatatc gagaatctca
    20821 tcaataattt cgagaagctg acgaccactg cacaatatgg tattcacgta atccatgcgc
    20881 tcgctgactt tcaggtcacg ctttaacatc aattcggcat atcctaagat ggcaccgata
    20941 ggagtgcgga tttcatgact catgtttgct aaaaattttg acttggtttt tgttcccgct
    21001 tcggcttcaa gctttgcgtt tttgagttct tcataggact cgatcaattt ctgctcagtg
    21061 agttttcgtt cggtgatatc atgggtcaca ctcaagaccg ctttggtctt ttcattgcta
    21121 aaatgcaaag gagccgaata gctttccatc caatgggatt tcccctgtaa atcaatcacc
    21181 ttgaactgcg cgattccgac ttgtcccgcg caggcctttt gatgcaaatc aaggaaagct
    21241 tcgcggtcct ctgggcaaat gaattcaatg acatttctac caattatttt ctcaggacac
    21301 tgcgaggcct ctaacatcgc aagccccgca ggatttatag ccaagaccgt gccatcggtg
    21361 gccacggtct taatacaggc cggctgatgc tgaatgattg ctttcaaacg ttcctgcacg
    21421 tgatggcact catttatttc aagcctgaaa gtagaacgaa ttttatccag ataaatcccc
    21481 agaaggccga gggacactga caaaagtaat aagaaagaaa gaagctcccc gccaaaaaga
    21541 aaaggtttcg tcgggccaag tccggccacg accccgcaat acaaaccccc taaaagcaaa
    21601 ctccctccca ctattcgacg agccagggag ttttgtagcg aagagcgaaa aagaagcatg
    21661 ccgctccaaa catagagagc gcccaagaaa agaaaaacag gggcctcctg aagagcgcta
    21721 agcagagtgg ttaaaaaccc aacagcgatc catattctca gctgtttcaa acttctttcc
    21781 gtaactaaaa aagagaggcc attggccaag agaagcgcgc tgctcaccat agctgcatgg
    21841 cgaaatatca gtccccattc cggcattact ttgagaacca taagaagttg cagaaaggat
    21901 gccagggtga aggcgaatcc actccgaatc cacagataga gaaaagattt gcgatcctga
    21961 gtgtgcaaat agaaataaag aacggtcatc acaaaactag tgataatgga tatacttatc
    22021 tggggaacca gaaattgcat ttattctcct atttgtttgt gaatatgaca ttatatcgag
    22081 taaaaaaaca ataggagcta gggtgttgaa tcatgtatat ccgaaaaaaa agaacagtcc
    22141 ttcatctgat aaggataata aaatgaattt tgagatctct tggcatgcct tgaatgtgga
    22201 cgaagttgta gatcaacaaa aatctcatat catccaggga ttatcttctg aagaaattaa
    22261 gcagcgtctc agtaaattcg gaaagaattc gttaccagag cccaagagaa gatctttttt
    22321 atttattttt ttccatcaat ttttaagtcc cctgatttac cttctactcg gggccgctgg
    22381 cattgcattt ttcattcata aagccaagga tgcccttgtt attcttatcg tggttttact
    22441 caatgcgatt atcggcgctt ttcaggaagg acgtgccgaa aactcgctgg aggctcttcg
    22501 tcaactctct aaattaaaag cccgcgtttt gagagaggga aaagaatcgc ttattgaagc
    22561 gagcgaactc gtgcctggcg acattctgat cctcaatccg ggcgatgccg tacccgctga
    22621 tgcacggctt atcacggcct cctcaatggc ggctgcggaa gcggctctta ccggtgaatc
    22681 ggttcctact cccaaatcca tctctccgct gccgtcgacg actgccctcg tggaccgcca
    22741 caatatggtt tatgccagca cttatatcac tgcgggtcgg ggccaggcca ttgtggtggc
    22801 tactggtatt tcaaccgaga ttggaaaaat cgcggattta accacgacca ctcttcaacc
    22861 aaaaactcaa ctggagcttc gtcttgagca atttggcagg atgctcgtca tcacagcatt
    22921 aattgtcttc tctttggtac tcggcctggg ccttttacac ggaattccct ttgcgcaaat
    22981 ttttatgatc gcgatcagtc agatggtttc attggtgcct gaaggacttc ccgtggccgt
    23041 gactattgcc atggctatcg gcgtacaaag aatggctcga cggggaacca ttgccagacg
    23101 tttaaccgca gtggaaagct tgggatctat cagcgttatt tgcacggata aaaccggcac
    23161 cctgactcga aatgaaatga cggtgaccac tatttatctt ccggccaaca atagaaaact
    23221 ttccatctct ggtattggat atgcgcctga aggacaactc actcccgagc cagataatac
    23281 attgaagaag ctttttgaaa tatgctgtct ttgcaatgat gcccacctat taacccccga
    23341 tattgccgga caatggcaaa tactgggtga tccgacggag ggggctcttc tcacgcttgc
    23401 cgctaaaggc ggggtcgact ccacactcat gcgcaaagaa ttcccacgca tcgctgaact
    23461 gccctttaat tctgacagca agatgatggc gactcagcat gaaataggtg gaaaaagcgt
    23521 tgtgttcatc aaaggagctc ccgagtccct cttggaactt tgtgattcca tttaccatga
    23581 aggatctttg aaagatttta gtaaaaattt gcgcgagcaa gtggaaaatg aagtcagcaa
    23641 aatggccgag aaggctttac gggtggtcgc ctttggattt atagaaaatg ccttcatcga
    23701 tgggtcaaag gggttttctc cattcgctca gaaaatgacc ctggtgggcc ttgtcggaga
    23761 actagatccc ccacgcgaag atgtggcgga atccataagg agatgtcaga tggcgggaat
    23821 tcgtcctgtc atggtcacgg gggaccacaa ggcgacggga cttgccattg ccaaagcgct
    23881 agggatcagt cgaaataatg atctcattat cgacggacag gagttggatc aattgtccga
    23941 cgaggatctc gcggcaaaaa tggatcacat cagtgttttt gcccgcgtcc atcccgcgca
    24001 aaaattacgg atcgttaaag cttatcaaaa aaaacacgaa gtggtggcca tgactggaga
    24061 cggagtcaat gatgcccctg cactggctcg tgccgatgta ggggtcgcca tgggaatcac
    24121 cggatccgaa gtcgcaaaag aggcggcaaa gatcatcata acggatgaca actttacaac
    24181 catcgttgca gccatttctg aaggacgact ggtctatcaa aatatcaaaa aattattact
    24241 ttttcttttt gtaacttccg tggacgaagt gacggtcttg tttttggcct tgatttttgg
    24301 gtaccctcca cccttagcgg ccgtccaaat tctatggatc aatcttgtga ctgaaggagc
    24361 acttacggtc aatctcgtta tggagccccc cgaaggggat gaaatgcagc gaccgcctat
    24421 ccccgccaag cagcctctct tagatagcac ccttctttca cgcattcctt tcatgggatt
    24481 ggcttcagtg atctccactt ttgggtggtt tgtctatcgc acttcgctag gagtcacccc
    24541 cgctttagtg caaactgaaa ctttcaccgt tctcgcgatt tgccagtggt ttaatgtgct
    24601 caactgtcgc tcggcactgc gttccgtttt ctctcgagac tcttttggaa gcttaaggaa
    24661 aaacccttgg cttttaggag gccttgcttt gggcatcacc ctgcacgtgg cggtgattta
    24721 ctggccgccc ctgggccagt ttttttacac gatgccgatt gcacccgcgc aggttcttca
    24781 aatcgtactc gtctcaagcc tcgtcttgtg gagtgaagaa ctgcgcaaat ttatcatgcg
    24841 aagacacctc ctctgagagg ggcccatata ttgcattttt taaaagaatg aggaaacaga
    24901 agccgaccaa cctaagtagg atacaaataa accaaaagga gtgcttatga agtccctttt
    24961 tttgtcattg ctgttaattt tatcgtcagt cattttatcg acagcaagcc atgccgatca
    25021 gtctgcaacg ccctcaacgt cgcccggctt tgaagatgtc ttagtaaggg ataaaaaaga
    25081 tttagacgct gattcaaata tgggattaac tgctagagaa cttgaggcca ataggttgat
    25141 gttcaagcaa aaggtcgaag agtttgccat gaatgcattt cgcactcccg aggggcgacc
    25201 cattatccag gctgaagcaa acttattggt taaatatttt atagaattaa aacttccgcc
    25261 ggaagcccta acctcttata cattccattc ggatttgaaa ttgcccttcg taggcaatga
    25321 agcgcagata aaggcccaaa gatctcaagt cattcagact cttgtcaata ttggccaaac
    25381 ccgtacggat atggacactt atctggtgga ggatgacgtt cgctgcttct atagaaattt
    25441 tagagacaca gacgctgtgg aagaagtcct taaactttcc gagaacgcta aaatgatgaa
    25501 cccgctcgcc gatatcttcc gacgctctga tgatgtcgtc attgtgggta aatgccaagg
    25561 ctgtggtctc cttaatatca aagggtaccc aaagactccc gaagaagaaa tgaacccctt
    25621 tagtgcctgc gcgggtgact cctattaaaa ccccattact tctttaaatg tttttccgca
    25681 tgaacttcat tgcttaaatt ctttccgttc gcaaactccg tgatatttcg cagagtggtt
    25741 tgcgcaatat ttactaaagc ctcctcggtg agaaaggctt gatgagaagt caaaagcaca
    25801 ttcggaaagg ttaacaaacg cgccaactga tcatcttgaa ggatttgctc agaaagattt
    25861 tcaaagaaaa tcccctcttc ctcctcgtag acatcaagac cggcagagcc aatatgtttg
    25921 gattttaaaa actgaatcag cgccggagta tcaatcaaag ccccccggcc cgtattgatc
    25981 agcataaccc ccgccttcat ctgagaaagc gctttaccat tgataagatg ccgagtctcc
    26041 ggagtcagcg gcacatgcaa ggaaatgata tcggactttt gcaaaagctc ttcaaagctg
    26101 acgtactgaa cacccacctc gataagttct ggattctgga cccggtcact ggctaaaatc
    26161 tgacagccaa agcccgccaa tattttcgcc atgacggaac caatttttcc cgtgcccaca
    26221 atccccgccg tttttttatg aagatcgaac ccgacaagtc catccaagga aaaattgcct
    26281 tctcggacgc gattataagc gcgaggaatc tttctattta aagaaaggat caaggccacc
    26341 gcatgctccg ccaccgcata gggcgagtat tctggaactc gcacaatacg aagatccagg
    26401 tcactggccg cctccagatc cacatgattg aagcccgcgg acctcagagc gatcaaacga
    26461 gtgccactgg cggctataat tctcaaggtc ttactgtcca agcggtcatt gacgaacgcg
    26521 caaacacaag gataaccggc ggccagattc gcggtttgct ccgtcaggcg tgtttcaaaa
    26581 aaagtgattt cgtgcttaaa ctccgtgttt tctttttgaa aaatatttct ctcaaaagca
    26641 tgagtatcaa agaaagcaat tttcattcag gtctccgttt tcagtatttt tagttttttt
    26701 tctctgcttc taaaaccact tggaaagttt tcgtcaccgc atcaggggtc actgaaatgg
    26761 agctgattcc ttctttgact aaaagttgcg agatctctgg ataatcactt ggcgcttggc
    26821 cgcagattcc gatgggacgc tttgattttt tggctccctc gatcaccaga tgcaacatct
    26881 ttaaaaccgc gggatcgcgc tcatcgaaga gatggctgac caccgaggaa tcccggtcca
    26941 ctccgagaac catctgagtt aaatcattcg agccaatgga aaacccatca aagatgcggg
    27001 caaaatcttc agcacgcaac acgtttgacg gaagttcagc catcacataa acctcaagtc
    27061 ccttttctcc gcgaaccaga ccattccgct tcatttcagc caaaactttt tctccttctt
    27121 caggagaacg gcagaaggga atcatgacct taatgttggt caaccccact tgctctcgaa
    27181 gatgtttaat cgcgcgacat tccaaagcaa aaccatcacg ataatggctg tcataataac
    27241 gagaagcccc gcgaaacccg atcataggat tttcttccga aggctcgaat tgctggcctc
    27301 ctagcagacc tgcgtattcg ttggttttaa aatctgagaa acgtacaatc accggccttg
    27361 gataaaaggc tcccgcaatg aggccgaccc cctccgccag cttttcgata aaatactgag
    27421 cggggttgtt ctttgaagat cccagcaaat tctcgatctg cgccttgatc tcaggctctt
    27481 tgatctcttc gaggtgagct aaagccatgg ggtggacttt cacatactcc gagatgatga
    27541 actcaatgcg ggcgagtcct acgccgtcca caggcaaaag agaagttttc agcgcctgtg
    27601 cgggatttcc taaattgacc atgatcttgg tgtggggctt ttgcaaagag gtccaattca
    27661 ctttatcttc gacaaaagac aaggccccct catagacaaa tccttcgtca ccttccgcac
    27721 aagagaccgt cactgtttgt ccgtctttca gaacttccgt agcgatccct gagcctacaa
    27781 tacaaggaac tccatgctca cggctaacaa tggccgcatg gcaagttcgt cctccgcgat
    27841 tggtgataat cgccgaagcc tttttcatga tcggctccca atccgggtcc gtcatatcgg
    27901 ctaccaagac ttcaccgttt ttgaattgcc ccaattcaga aacatctttg atgatgcgga
    27961 ctgcgcccgc gccaattttt gaacccacgg ctcttccaga aatcaaaacc ttcgatcgct
    28021 cttttaaaac aaaagaactt tgctgcacgg ttgattcttg ggagtgcacg gtttctggtc
    28081 tggcctgcaa aataaaaaga tcgccggttc gaccgtcttt tccccactca atatccatgg
    28141 gcgtatcatg gccatgcact tttgaataat attgttcaat ggccaaagcc cactccgcaa
    28201 gttttatcac gtctccatca acgagtgaga gcttttcacg atccgcttga gaaacttcga
    28261 tatttcgcgt ggtgcgactg ccatggcctg aatagatcat tttgacttgc ttcgctccga
    28321 gctttcttcg caagaggggc agagtggctt ttcccaaaag aggtttgagt acaaagaact
    28381 catcgggatc gacacggccc ccgaccacat tttcgcctaa accataggcg ctgttaatga
    28441 gaatcacatt tcttgccccg gactccgtat ctaaggtgaa aataactccc gaggcggcta
    28501 gatctgagcg caccatctgt tgcacacaaa tagaaagtct caccttggcg tgctcaaatc
    28561 ctttgccggt tcggtaactg atggctcgat ccgtaaaaag agaagcgaag caattcagac
    28621 aagccaccag caaatcttca gcgccacgaa tatttaaaaa ggtttcttgc tggcccgcaa
    28681 aggaagcatc cggcaaatct tccgccgttg ccgaggatct taccgccaca tccaattctt
    28741 ttatgtcggt gcgcagacat aattgagtat aggcttcgcg gatttcctga gccacgctct
    28801 cgggaattcc cgtcttacga atcaaagatc taatttcatg ccctgtcctt gccaattcat
    28861 cattatcatt gatgttcagc ctggccagcc gcgaataaat ttcttcagaa attttatttt
    28921 gctcaatcag aacagagaac gcctccgcgg tcaccgcaaa gcccggtgga atgcgaatgc
    28981 cctgaggggt cagtttctgg aacatttctc ccagcgaggc attcttgccg cccactaagg
    29041 gaatatcttt gatagaaatc tcttcgaacc atttgatgaa tttcggactt gattttgaat
    29101 tgcttttttc cataggattt aattgatact accatgactt tctaaaaaca aagttttttg
    29161 ataaaatttt tgtttactcc aaagaatgga gcttgcataa gtcgcgaaag cgcttggtat
    29221 tcaaaaagga attagtaacc gcgaactcac aatccacccg gccaagtctc ggggaactct
    29281 ttgatcgagg tcaaagttgg tatcgagcgc agcgcggtgg tttgatcgac aaagacaaag
    29341 gcatcataac gtttcgccat cactgttggc acatagttac gcccgtgaac ttcaaaacga
    29401 ggatgataaa ccactcccac agcgcgatgc ccatactctc gagttcctaa gatcgtcttt
    29461 ctcgcgtcct gatcgaaaag catgtaaaaa cgtggcgcct gaagattctg cgaaacttta
    29521 tgacaataag attcaaagga attctcctgg gctgcgggca aaggagtgac cgtctcaggg
    29581 ccatcccagg ctgagctggc taggacctcc ccttgataag tgccaaatcc caccaaggcc
    29641 acttgctccg agccgaatct ttcacgagcg agtccaccta gattgacata gccttcctcc
    29701 agcatatccg tagcatgata atcaccgata tgactattgt gcgcccaaat gatgcactta
    29761 cgattttgaa aaggtcgatt taataataac tccaaagtct ccatcatatg ctgatcacgg
    29821 atattccaag actccgcccc gccagacaac atggcccgat aatactcttc agcgtttttc
    29881 acaatgcgag cgttctgttg ggcgctaaaa agatctccgt ctttaacttc caggtccgcc
    29941 aaacgcaacc tcagtatttt tcgaagattc tcaaccactt cacggcgaca cccttcttcg
    30001 aattttaaaa gagattgagc ataggctttt tcgttttttt caaaaggttc aaagcaagag
    30061 tacgcagctt tcatttcttg agccagctcg gggcttatcc gctgtatgta ggactgcaca
    30121 tagtccatcg attcaaaaag agaatagaca tcaagaccgt aaaaaccggc tttataattt
    30181 tgcatccatt ctataagggc cgcgacctcg tcattggccc acatccaggt cggccaacgt
    30241 tgaaaatggt gcatgatatc catcgccgtc cgagaagaac tcttcccttg aatataatca
    30301 ttgagttttt gagaatctgg ccagtcccct tcgacggcaa taaaatcaaa ttcgtgatcg
    30361 cgaattaatt tttcagagat catgcgccgg atatcgtaaa actccgcggt gccatgggtc
    30421 gcctcgccca acatcacaat ccgcgacttc gccagctttt caataagatt gtaccaagcg
    30481 ccttcgctgt gaatcggctg agctctttct tcaatcgctc gaacaatatg ttccttaggc
    30541 ggcagaggcg ctttcggcct gggagcatat tgcaaaagcc agtccgccgt atactccacc
    30601 acttccgcga tggcttcgag ctctataatt atcaccattt gcaccgttgc caagcgttgg
    30661 gcagccaatt cattgagagt gatcacctcc agatccgcct cgctcaccat caatagcgtt
    30721 ggcgatttca ctcgacgcaa atatttattg accagatccg gacgtccgcc atgactaata
    30781 attgcaaaaa tatcccggga actttttgca gcggctccaa gtgccaccgc agcccccgtg
    30841 cctgttccaa agtaagccaa agaagaatgc accccatgca cttgggccaa gtgaggaatg
    30901 acggcgtcag aaactttcaa caatcgatct atcaaaagtt ccatattgaa aatatttttc
    30961 cgatcctctg attctctttc agttaacaaa gctggcacaa gggtggcgaa gccgatttta
    31021 ttgagttctt cagccacctg taaatttcca cgactatgac ggctgccttc gctttcgtgg
    31081 ataaaaacaa ccagtccctt catattcgca actaattgga gatctgcttt gagctctatc
    31141 gtaccgtcat aataagtcat ctcttttccg acgatttcgt cgggcacgac tttgccatgc
    31201 aataaactta tcacctcttc atctgaaact tgagaaaaat cctgatacca atcccccact
    31261 gcaatgaagg gaaagggcgt ttctaaacac accacttcgt ccgcaatctg acggagggat
    31321 tccagcgtcg cctccggagc caccggcacc gcaataataa ttttttccgg ttggcgctca
    31381 cgcagaaggt gaatagctgc acgcaaagtc gctcctgttg cgatcccatc atcgacgatg
    31441 ataagaacgc ggccttcgac cggaatagcg gggcgtgaac cacgcaaccg ccgcacctgg
    31501 ctttgaactt cttccagctt cttttccacc atcgcttgga tatcagcgtt cttgatatta
    31561 aaatggttga tgatcttgga attcaaccag ggagttccct cctcactgac tgcaccgatc
    31621 gctaactccg gattctcggg agttccaatc tttttgacaa tgagtagatc caaatcagct
    31681 tgcaactttt tcgcaacctc ataagccaca ggcactccgc cccgggggat ggccaatacc
    31741 agaggctttt cttttttgcc gcccttcgta ccttgcttca gagagtgcaa taatttgtca
    31801 gctaaaatga ttccggcttc tattcggttt tgaaagcaca ccatagaaga ctccttatgg
    31861 tgaatgggca agatgccatt gtcaccgtta tcgtctctag ggaatgagaa aaaggaatga
    31921 agcaaacttg aaaaagttgt gaattataac tcgtcaggca gccaatgtgc gtttttgcac
    31981 aaagttgttt ggtacttggg tctaggagat gaatggcgga ttgtccttcc cccatttatt
    32041 tgccagcatg aagaaagagg aaggtagaat atttttggaa atgggtaata atgcatttat
    32101 gggatcattt tcaaataaat ttataaaaaa aatagctttt tcatcaaaga tcttcgtgag
    32161 agggttgagt ctccacgcaa ggggatagcc ttagctcatt aattagacaa acagctttag
    32221 gaatgaaggt gccatttagt gtatcagata ttcaaaaact ccagccaagc atagaccgcg
    32281 agcttattaa gaaagtttta ttcaagctgc gcgatgaaaa attgatcttc cttcaaggaa
    32341 aaggtcgaag tgcaaaatgg aaaaaaatct agtcacccgt ctttcggatt atttaaacga
    32401 accgattttt taatactttt ccgtgagagg tccctttagc catccatgga taaactgttg
    32461 caccctgggg tccggatgat tttgcgtctg cacagcgttg ccggtttcga taactttttt
    32521 gtcggccacc agtaagattc ttccggcaaa ttgataagcc atttgcatat catgggtcgc
    32581 gatgatcaaa gtcgccgcca cttctttttt taaatttaaa atcatctccg ccataagatc
    32641 gctattgact ggatccagtc ccgcagtggg gtcatccaaa aaaagaatct ctggcttaag
    32701 cgctatggcc cgcgcaattc ccagtctttg tcgcatgccc cctgacattt catggggcag
    32761 aagactttta gcggcttcca agcctacggc tttcaaacat gccaacaccc tgtctccaat
    32821 gacttcatca gagtccctgg tgtgttctcg caagggaaaa gccacattct caaaagcggt
    32881 caactcgtca aatagcgcac ttttttggaa ttggacgccg atctttctag ccacgtcatg
    32941 tttttcgctc atggatagag cttcccactc atgaccatag cataaaactt ttcccgtggt
    33001 aggagtgtaa attcccgcca tggtctttaa aagaacggat tttcctccgc cactaggacc
    33061 gataataaca attgtgtcat cttgatgaat agaaagattc acaccgtcta aaataatctc
    33121 accaccaagt tcaagagtga cgtcgatcaa ttctaaaatt ggagcgcaaa ttggagcgca
    33181 aattggagcg ctctgacgag aagatgagtt tttcacatat gaattttact tcgaataggc
    33241 ttgtctctgt caactatcat agttagtatt ttgcttaaac acagaatagg aatttccatg
    33301 caaagaatgc actttatttc aggcttacca cgatcaggga cgacgcttct ttctacagtt
    33361 ctcaatcaga atccaaaatt ctccgcttca atttctggtc ctgccgcccg catggtaaga
    33421 gccattgtca ctgaagccca agcccagggt ggttaccgca ctcaatgtcc tgaggggaaa
    33481 cgtcgcaaac tcattagaaa tttattagag acttattatg aagattcccg tgaggtggcc
    33541 tttgacacca accgtggttg gacactcatg acgcctcttc ttcgcgacat ctacccccat
    33601 gccaaaatga tttgctgtgt gcgggatatt ccctggattt tagatagctt tgagcaactg
    33661 ctacgcaaga atccctattc ggtgagttcc cttttttctt ctgaagaatc cgtggatgtg
    33721 tactctcgaa cctccactct attgcgggcg gatagaacca ttggctttgc cctgaatggt
    33781 ctcaaacagg ccgtttatgg tggcgagcgc gacatgatct tgttggtgaa ctatgatgac
    33841 ttctgccgta ctcccgaggg ggtgatgcgc gatatttata aattcttagg agagtcttat
    33901 tttgcccatg actttaataa cgtcgaggcc tcttatgatg agtttgacca ggatgtgaat
    33961 ctcaaaggca tgcacaaagt ccgcaaggaa ttaactcata tcgtccgtga gccgattctc
    34021 cccccagatc tgtggcacca agcggacact ctctctttct ggaaagctgg acgaatttaa
    34081 tagggggccc ctattctatg gaaaagcaag cgaactcttc tgcacaaaca tcacgtgaac
    34141 tggggctggc tgccgcctat gccagcaaat ggatggaagc tttacttcac ttaaacgcgg
    34201 cggttcggca aggtcattat gatttaatgg tgttggatgc tttgggcgaa gctgcttaca
    34261 gagccaatgt cccagaggct ttaggacctt atcaaaatca ctataaatat cccaagatcg
    34321 cttcgcatat ggcgagggct ttcctgatgc tcggagatgt cacctcttct tatgaatttt
    34381 tgggctatgc tcaagactcg gcgttgaaat cggccttgtt cgcgatgctc gaattaggtt
    34441 ctgagattac cacgaccgca gcccaaatgc tccccgtcgc tgaaactcat cctgatcttt
    34501 attatccaga atactggcga gccttgtcag cggtcgcgga tgccgtcgga agagaagatc
    34561 tcactcgtct gtcagagcga aaatcgaaag actttgctta caaagatccc aatatccatt
    34621 tcaatcaagc cttgcggatg ttagggaaag gagaatttcg agccggctgg cgcctttacg
    34681 aatggcgctt ggttcccaat gccgctcaat ccaaccgcac ggagcttggc agaatagcga
    34741 tgtgggaagg agagcatctt cgaggtaaaa ccttgttaat atacttagaa caaggcttgg
    34801 gtgatggcat cttcgctctc agatatattc cagctctttt agaacaaggg gtttgtttgg
    34861 aagttgtcgc aaggcctgcg ctgcttcctt tgattcaagc aagtcatcca aacgtgaaaa
    34921 ttcataatga agacgaggtc gtcgtcgtcg gatattggga tcaagtgcct tctcctgatt
    34981 tttgggtgta tgggctttcg atcccctttc gcgcgggctt ttggcgtcct atgaatacgg
    35041 cgaaatttct gaaggctccg acaaccgtag tggaaaaatt taaagaaaga attcttcgcc
    35101 agaacttgct ccatttgccg gtttacaccg tgaattggca tggacgcatc gacacggagt
    35161 cggatcgaag ccgtgctttt tccgttcaag agttcacaca ggtcacaggt ctcgaaaaaa
    35221 atccttgcct cgtcatctct ttacaaaaag acgcctcgga ggaagaaatc acgtgcttgc
    35281 aagaactcgt cggaaaaagt ggaggccagt ttataaatgc ggctccggag cttggtgatt
    35341 tcgccgcgac cgcagcctgg atagccgctt cggatcgttt gctcacctgt gacacctcag
    35401 tggcccatgt gggcggcgcc ctcggccatc ccaccacggt ctatgttcgc aataaagcca
    35461 tctggcaatg gctacggaaa gatcctgaaa agaccagcgc cgctctttgg tatgagagtg
    35521 cgcttgttca atttgcgtta gctccaaaaa tttcttggct ttttacaacc ctcaataaac
    35581 cgaagagtaa aatgaaggaa tccatcgatg acccatcccc atcccccctt aaagactctg
    35641 agttttcatc aacagaaatt cgccctgcaa gacaccgagg gccattcaat ttcgctggtc
    35701 gggctgaata attggcaaaa tattccgtgg aaagaagcgg aaatcagcgc catcgatttg
    35761 ggcgacctgc cctgtcgatt aagtcgacct gatctggcgc ttgcgttgca aaaaatctgg
    35821 cacatcagca aggaaggagc ccagataaaa ttacgcattc cgcacccgcg gcatgacgtg
    35881 tttttagcgg acttgggata tgtctctgcc ctgttgcccg aaacttttga acatctgaat
    35941 cagttaagta taagatttga aacggtggaa gccgccttca tcttggaccc ccattggaaa
    36001 gcggctctgg atgaaaagaa agttactttt gaagatattc agttcatctc caaacaagcc
    36061 tcgaatgtga ttcaatggat agatctccag ttgcaagtta aaaaatccct gtgggtggaa
    36121 acctctttct ctccgctgac cacagccatg agacaacaat tgcagtctca actccaagca
    36181 catatcgcgc gaggtgacga aaaggccgct gctattattc gccaatttct cgacagtccc
    36241 gcgaatgcac cccttgaaga aaagagcaaa atatgaattt actaaaattg aatttgggct
    36301 gtggatttga caaacttcca ggctatctta atgtcgacaa agaagccgct tgcgaacctg
    36361 atcaagtggt ggatctggaa gctacttggc cttttgaaac caactccgtg gcggaaattc
    36421 gcgccgccca tgtgctcgag catctgggcc aggaaacaaa agtattttta ggtctcatga
    36481 aagagatgta ccgtgtctgc gtggatgacg cgaaaattta tatccaagtg ccgcaccatg
    36541 atcattggac cttccatgcg gatccgaccc atgtgcgaaa aattcttccg gaaggactga
    36601 agcttttcga tcaagaactc aatcgccagc atattgaaaa taaatatgcc aattcggctc
    36661 ttggaattta ctgccaagtg gatttcgttc tcgagaaagc cgtgccttct tttgatgaac
    36721 cttgggcttc acgaattcaa aataatctag tggctcaata tgatttaaat ttcgcggcca
    36781 atcactatac caacgccatt gtccaatggg atattgtttt gcgagtgcga aaaagatgaa
    36841 agaaaaaatg aaagtcagta atgataaaaa ttttaagctt tggctcgaag cggagaaagc
    36901 tctcagtgta ggtcttcccg ccggaactgc tcccgaggtc ctgcgaactt caggtctatt
    36961 gagggcgcaa agtcaggctt ggtcggcagc gctgctcaga ctcgaagaag ccgtccgcaa
    37021 tggaatctct gatttgcaaa ctttggatgc tttgggagaa gccgcttatc agactcaaac
    37081 ctatcaggca cttcttcctt ttgaagatct ttatcgagat cccatgattg ccatccacat
    37141 ggctcgcgct ttgatgatgc tcggacagat ttccgccgct cacttcttct taaggctcgc
    37201 gccagattcg ttgttaaaag ccgccatctc agctgtcctt ggagtggaaa aaaatattga
    37261 aacctctatg acggctctat tcaaacccct cattaagaaa gagatggaaa ccgtcaattg
    37321 catcgaatac tggcaagcct tggctccagt ggctgaagtc gctggcagaa aagatttagt
    37381 ccacttggct gagcaaagac tcaaagcatt ggcctacgcg cgccccgtga tccattacaa
    37441 tcaatcccta cgcttattgg cagagggtga attccgcgct ggatggaaac tttacgattg
    37501 gcgtctagtc ccgggctctg cctgtcctgc gatgacaggt tttgcggatt tttctatgtg
    37561 ggaaggtgag tttttagccg ggaaaaaaat attagtggtg atggagaatg ggcttggaga
    37621 ccaaatcttt ggtctccgtt atctccagac tctcgcagag gaaggggctg aagtgaacgt
    37681 ggccgtggga ccagaattgg cagctcttgc tcagtccagc tttccctcca tgaaaattca
    37741 tgatttagaa aaagcccgtg agctcaccta ctggaaagat aaatcacgcc ctgacttttg
    37801 gacttactgc ttgtccattc ctgcacgggc ggatttgggg gatcccctcg cgacagcggg
    37861 gtatctgaag gcgtctgaaa gtttggtgct tcaatgccaa cgatccttga atttgcaaaa
    37921 tccaagaaag ttgccggtcc atggattggt ttggcatgga gatattcgaa ccgctccgat
    37981 gcgcacccga gcttacacgc tgacagaatt tctgcgtgaa tcagaaattc ttaaaactcc
    38041 ttgcctgctg gtttgcttac aaaaagatgt cactcaggag gagctttccg agttacgggc
    38101 tcaggctgat aagaggggtt gcctgttatt caacgcggct ccaagcctgc agaatttcgc
    38161 gcaaacggca gcctggatca attgcgtgga tcatatgtgg agttgcgata catccaccgc
    38221 tcatttggcc ggtgccctgg gggtgcgcac tactgtcatg atacggaata aagccatctg
    38281 gcattggcgc tgtgaggcaa agactcagaa ggctgtgtgg tatgattcgt gtcacataaa
    38341 atacgctctc actccgccta tttcttatat gtttgatatc cgcgatccgt ctgattcaga
    38401 agggaaagtt taggataaga atgacttcaa tatctcctcc gccgcctgct caggattgag
    38461 cgctgaagta tctataatca tctcaggatg tggcggtatt tcgtagggat catcaatgcc
    38521 ggtcatggat tttataagac cggccctggc cttcttataa agacccttgg ggtcacgggc
    38581 ctcacaaact tccaagggag tatttacgaa aacttcgaaa taatttttac aaagctttct
    38641 cacgaacgca cgggagtctt caaaagggga aattaaactg gccaccacga tcaccccgtg
    38701 tgcctctaaa cgacttgcca taaagcccac tcgtcgaaca tgttgatcgc gatccatgcg
    38761 agagaaacca gtagagggaa agacttctcg aatggcatcg ccatccaact cctcaacttc
    38821 acaacctaga gaacgcaatt tctttgccaa agtttgagcc accgtggatt tcccggcagc
    38881 cggcaagcca gtgagccaaa tgactccgga ttttatttcg caagaaccca tacttccccc
    38941 tctactttca ccattaatta taaactactt gcgacccaat cactttgcaa ataagtttaa
    39001 gttagctatg aaaagaacac ttgatcatca tctcgattgg ctggagtccc aagctattca
    39061 catcctgcgg gaagtggcgg ctgaatgcgc tcgtcccgtg ctgttgtttt caggcggaaa
    39121 agactcggcc gtcctcctgc gtattgctga aaaagcattt cgacccggtc cctttccttt
    39181 tccactattg catatcgata ccggccataa ttttccggaa gtcttgcgct ttcgcgacca
    39241 acgcgcggcg gagttaggtg aacacttgat tgtgcgttcg gtggaagact cgatcgaacg
    39301 cggcacagtg cgtttgcgaa atcctctcag cgattcaaga aatgccgctc aatccatcac
    39361 tctgttagag gcgattaacg aatttcaatt tggcgcttgt attggtggag ctcgtcgcga
    39421 tgaagaaaaa gcgcgagcca aagaacgcat tttttccttt cgtgatgaat tcggccattg
    39481 ggaccccaaa aagcagcgtc ccgaactctg ggatctctat aatactcaca ttcatcccgg
    39541 cgaaaacatg cgcgtgtttc cgatttcaaa ctggaccgaa ttagatgttt ggcaatacat
    39601 cgaacgtgaa caattgactt taccgacact ttactttgct cacccccgcc aggtcatttc
    39661 gcgcaagggt tggttagtgc ctcacactga attaactccc gctcaagaag gcgagcccat
    39721 cgaaacacga atggtgcgtt tccgcacggt tggggatatt ccctgcactt gtcccatcgc
    39781 ctcagaggcc gcaaccgtcc aagccattgt cggtgagata gcgctcaccc atctcaccga
    39841 gcgcggcgcc acccggatgg atgatcagac ttccgaatcc tctatggaaa aacgcaaaaa
    39901 aacaggatat ttctaatgat gacttctgtt cttcgctttg tcactgcagg ctctgtcgac
    39961 gatggcaaaa gcaccttgat cggtcgcttg ctctttgaca gcaaagggat ttttgcagat
    40021 caactggaag ccctcacccg cgccaagcac caacgaacct gcggagagga catcgactta
    40081 tctttactga cggacggctt ggaagcggaa cgtgagcaag gcattaccat tgatgtggct
    40141 tatcgttatt tctcaactcc tcgacgtaaa ttcatcatcg ccgatgctcc aggacacgaa
    40201 caatacacgc gcaatatggt caccggcgcc tccaccgccg atgcgatgat tctcttaatt
    40261 gatgcagcga aagtgaaaga gagcttacaa caaggccagg gcctacttct tcaaaccaag
    40321 cgccattcca ccattgcgca gattctgcaa atcagacata tcatcatcgc catcaataaa
    40381 atggatttag taaatttcga tcaaaacgtt tacgaatata tcatcaaagc ttatgcggaa
    40441 ttcgcccagt cgctgggttt aagtaatatc catactcttc ccttatcggc gctcttaggg
    40501 gataacgtgg tagtgagcag caatcacatg ccctggtaca aaggaccctc gctgattgaa
    40561 ttgcttgaat ctttaagtct gtacgatgcc aaagccaagg accaagcttt gcgttttccg
    40621 gtgcaatggg tggcgcgcca ccaaggttcg gagaccaacg attttcgtgg ttacatgggc
    40681 cgtatcgaag ccggcaaggt ccgcaaagga gagactctca gcgtccaacc cagcggacaa
    40741 agcgccactg tcacagaaat ccttagcttc aacacttccc tggaatccgc tgaatctgga
    40801 caatctgtga ctcttttact caaagaacac ctagatattt cccgcggtga tatgttggtg
    40861 agcgcaggac atccgcccac tttactcaaa aatgtgcagg cggatatttg ctggctctcg
    40921 gaacggcctt tggatctgcg ccaccgctat tggcttaagc atacctcaaa gcaaactctg
    40981 gcgcgggtga cggatattca aagttgcctc gacatcaaca ctcaacaacg gcatccaact
    41041 gaaagtctga agctcaatga catcgctcgc atatcgcttc atctgcaaca gtcgataagc
    41101 gccgacgcct atgactacag tcgtgctact ggagctttta ttttaataga cgaagtcacc
    41161 catcaaactg ccgccgccgg tatgatccgc ctcgatacgg gagctcaaaa ttaggaccat
    41221 caatgaatac tatgataaaa gcgaataaag tggatgttgt attaagaacc tgcgcgcgaa
    41281 ccggtgtttt taatcagaaa atagtaccca gaattgtttc ggaggatcgt ctcaccctga
    41341 tcagaacctg tatcaaatct ttaattgtta gcattaataa cagcgacaaa gagatcactc
    41401 tcaccgtttt cgatgatcat tcggaacccg cggatcttga aaaaataaaa tccattttag
    41461 ctgaatgcaa agttccgacc caagtattca cgacgcaagt ccctggattt aacaactccg
    41521 ctttagagca atttaaatat gggcgcgatc atgggcgcga actggtttat tttgtcgaag
    41581 atgattattt gcatgatcct caggctatag atctcctggt caatgcctat aatcatttta
    41641 gaaatttaag tgggtttagt gaagtggcca taagtccctg ggaccaacct caggaatacg
    41701 atgtggcaca tgctcaagct tgcaaaatat ttcacttcca taatcggtat tggcgcacca
    41761 cgattagcac ttccaataca gttttgtggc ccctcagcgc cgtcaaggga ggctggcact
    41821 tatttgaaac tttggcctta caatatggaa acgtagccgg tgttgaagaa atcaatacca
    41881 ttggtcaact cttaaacaac acggttcacc atcgggggcc ggtgtgctgc ttttctccga
    41941 ttccttcggt cgctctccat attcaccatc taggccaggg ctcgccgaag tatattgata
    42001 cgcaaatgtt tgattggcag aaatgctggg acaaaaaaaa gagcgactta aaataagtcg
    42061 ctcttttaat attcattatc gatgttcttc ccgcctagtg acggctatcg gagtctccac
    42121 cgccagcggc tggtcggtct tcacggcgat cctcgtgtcg ctcttcgcga tctttgcgcc
    42181 gttcttcacg gcgatcctcg cggtctttgc gtcgttcttc tcgattctca tcgcgatcct
    42241 ggtctttttg ctttcggtct tcttttttct cctcaagttc ttttcgcttt aattcccggc
    42301 gctcttcatt tcgcttatga gccgctgcag ctgttcgttg accgaagttg tcttttttag
    42361 tcttagtcgt cttgtcttta tccttatcct tatccttatc cttatcctta tccttatttt
    42421 cttgctcgct gactgcacgc aaagaggagt caaacttctt tacatcttca gttgttgccg
    42481 gagcgacgtc gatagcgtag gctttaaaag agaaagtaca aagaaccgcc ataagagcca
    42541 atttgaaatt gatcttcata tgattttctc ctgattgaac gcagccttct gtggttcaga
    42601 aaactggatc gttaatattt tttccttaat aagtttgtca ttttcatcaa agacatggat
    42661 cttaaactgt ttaagtcctg cgctcttcgc ctccactaca aagggcaatc ttgacttccc
    42721 aatctcaaat cggtcggcgc ttaaactcaa agtcctcttt tttgcaatgc gcgcatcgtt
    42781 cgtatgaaac tccacaccct cgggaagttc catctccatg cgcacatttt cataagcaat
    42841 gccggagtca ttctccaaat gaaatacaat gggattatgg actggtatcg cagagctggt
    42901 tactgactct ggctgatgct ggcgaatcaa taaaaccgcc aaaaaaccta agctcgctaa
    42961 actcagaccg cgccagaaaa aaatctcacg gcgatagctt ttagttatgc tattttttga
    43021 tgaaacttca gcccagacct tctgtgataa ataaggatct ggaaccaagt cttcatccag
    43081 ctgttttaaa atgttttgaa cttttaaatc tctcatatgt ctctcgtttt tctgctctcg
    43141 cattattaag acgcgcgggg cttgggtaaa gttgcagctt cttcccaaat ttttacaaat
    43201 tcctgacgcg cacggtgtat tttcgatttg gcgttagctt cgcttatttt taagacactg
    43261 gccgtctcag aataggacaa tccctgatgg tctaccaaaa gcaacagaag tctttcctct
    43321 tcttccagtt ttagaaggca actccatacc tcaaaagaag ccggcagggt atgccgagac
    43381 tcggccaggc tttccttggc aaaggaatcc tcgtatttgc ggtgtctttt gttatctaaa
    43441 aactgattcc tgcaaatttt taaaatccac ggaagagtac gtgtgacatc tcctagggag
    43501 tcccactttt cccagacttt gataaagaca tcttgagtta ggtccttagc ctcggcttga
    43561 tttttcgtca gaaaaaaagc gagtctcaat accgcttgct gatgctcttc aatgatcttt
    43621 tgaaattcat ttttggagat cattaccagc tccactgcag accaaactta cttataagtt
    43681 cggcatagtt tttgttttca atgtcgttcg tgcccaaggt tgattgatta ttgaaaaaat
    43741 tgaaagatgc aaagccgctc caactggaac taaatgcata ttttatcttt aggtacagat
    43801 ttgtggtcaa atcatttctc ttaacgttag aaggagtgga aatcctgtca taatttttag
    43861 aggtcacagc catacgtccg tatattttcc acagatcgcg gagcgcgatc acagtatata
    43921 agccgctctc cgttcccgag taagccagcg gcaaaattcc agtctgtgta cccacagtca
    43981 gtggcatatc tcctgttgcg gtccgcagcc ttgccatata gagttgcacc atcactgcgg
    44041 tattattaag ttccagaaaa agtttcaatg tttgcaaatc gccattcata taattttccg
    44101 ctgtggctag actccgctct tctgaaatcg ttccgctaaa gcccaaggcc catctgtcat
    44161 aatccaattt taccaaaaaa tcagaaccga gcgaaaggag atcaaaggtg gtttgagtaa
    44221 cttcaaaaaa aggggacact tcaaaaagcc agcgcgctga attgtaacct agatccaact
    44281 caaaaaaatg gtctgtggta tttaaatcac tgttttttaa atatcgactg tgcttgaaac
    44341 tataacctgg aatcagatag gattgaggtg acaaatcacg gcgatagttc acttccacat
    44401 tgaaattagt aatgctcgaa gccgtggccg atttcacctg ataaatattg ctatccgagc
    44461 ccacgtcgaa aagtatttct ccactgaatc cgccaggaag cagtggtttt tctttttcat
    44521 tgagatccgc agccagcgca cgggagcttt cccgaatatc cggcctctgc gtgtagcgct
    44581 ctgtaagttg taaatatttt ttcgcttctt cattcttatg aagactcttg taactcattc
    44641 cccgcaataa gaaagcctca ggagtctcgg aaagatctga cttttcgatt tcctttaaac
    44701 tcttttcata atccccttca ttaaagtatt tcaaggctgc aactgaagcc tcatcttctt
    44761 catcgtttac ggctttctta aaatgaaccg cttttgcgcg aagattttcc tggcacttag
    44821 aatcttcaat gactgtggtg acgatctcca cggctttttt cgaccggccc atattattct
    44881 cacacaccgc ttggtaatag gctgcgggta aagataacga cccatcccat tcccttactt
    44941 tcttgtagca gtctgaggcc ttctgaaaat tattgagttt ataatgtgta tgacaaaggt
    45001 tgaaccaata aatctgtcgc ttcggctcga gttcgacgag ttttaagaaa ccagctctcg
    45061 cctctatcaa gtgaccagac tgaaaatctt taatagcttc tttaaattga atattctccg
    45121 caggttcgtt tgaatcttga gcaaatgata tgcactccac caaactggcg aatacgatta
    45181 aaaatatcat tacagtctta tccatccacg gcctctgttc aatcccgctg aacacttact
    45241 tttcggaata catattgaaa aacgaatggt tcaaagtctg gattgtcgcc gtcttatttg
    45301 tgctggccta aacacatggc cctggttttc gacctaggca aggcgcttcg ctttatttag
    45361 attgattttt tcggtctaga attgagggac tttctatttg caaatataca agtctccgtt
    45421 agagtctcga gtgatcgctc ccgtcaagcc ggcacaagtg gttaccccag aagtgatagc
    45481 cccactattt cccgataatt gaacaacggc gctgctaata acagttttcc ccgttccgga
    45541 ggccgtaata gtaacatctt gattggttcc accagccgta aaggtcagcg ctccggtacc
    45601 agtaatagaa ccattcgaag tgcccgtacc gccattggca acaggtaaaa tgccagagac
    45661 atccgtagtt aataccgcgg ctgatttagt ggaaagcgta cctaaacctg agacttgagt
    45721 attcgcaatc gcgatattag cacaactcat aacatcccca actgaattat aagtcagtgt
    45781 ttggctagct gaacatgatg aggcaaaaga gttaacgccc gtgactgtgg atcgcaaatc
    45841 ggtaagagca ataaatcctg cggcccaagc agatccattc cagcgaaggc cttgtcccgt
    45901 cgtactacct gcggtgatgg agatcactgg agttgtggtt cctgtcgcca ccgagatcgg
    45961 tgcagtacca ctcacacttg tcacagttcc cccactcccc gtcgccgcga tcgtcacgct
    46021 gttggcccca tgggtaactg tcactccact gccggccgtg atggtcttat actccaatgc
    46081 agtggcaccg ttgttcatgc caagaatttg attagccgta cctgccgagg tcaatccagt
    46141 tccccctttg gtcgttggca ccgtatttaa agtcacagca ccagcatcac tgacactcgc
    46201 aatatccccc gacagtgaaa ctgcagccgc tgcaccagaa gcattgccga cccatatttt
    46261 agcggaggcc agactgggaa gagacgccga aggggtcgcc cacgaaagag tcggagtgct
    46321 ggctacgcca tcagttgtta agaattgtcc tgcacttcct tgagttgtag ggagagtata
    46381 agttgtagac ccagccgccg ccgctggagc aaatccaaca tagcctgagg tcgaaccaga
    46441 taatcttaaa gtccctttta catcgagaag ggatccgggt gttgtcgttc cgattccgac
    46501 gttgccatta tttagaatag tcatgcggct ggtgttgttg gttttaatat ctagattgta
    46561 gttgtcatta tttccaatgc ttgagtttcc ggcaaatgta ttgccgccat tcacaaaacc
    46621 cgtgctggaa ctcaaggccg ttgcccaggt taagcttccg gctccatcgg tttgtaagaa
    46681 ctgacttgcg gagcccgcgg agcttggcag agttaaagtg gtgtttgtag ccgtattggg
    46741 ataaacaata tgtgccactc cacttccggc tccaccgata tcagtgcctt tggtcacggc
    46801 cacagagctt gccgtgagtt gcaggatttt actggctact gcactttgag ttttatcaac
    46861 cgtgacgaca cctcctgaag tcatcgtcgc atcgcctgaa agagtttgct caaccgcggc
    46921 gttggaagca tttccgaccc acacttttcc cgctgttaag cttgcgctat cactgacagc
    46981 actgagtttt gcgttaaaag catcgtaatc ggttttacta ataagacccg ccgtcactga
    47041 tgccgttgaa gccatcggga tatttaaagt gtgcgtgctt gtagcagagc taaacgccgg
    47101 agagttgcca gaagtaccaa tggcaaaagt ttgagtgccc gaagtaagac cgttaagact
    47161 ggtgattcca actccggcca cccctaaaga ctgcacactt gagccattgt agtatttaat
    47221 cgtacctgag tcgtaccaca tttgaccggc aacggtgctg ggcgtggcat tagaagaaag
    47281 gccaaagtat ttattattag ccatggtgat attcccggca ttggtcatat caaagccacc
    47341 catagcgatg gctccactca tcgtgccccc agcaagcggg agcttggtcg catccagagg
    47401 ggctgccgtt gtcac
//
LOCUS       DATBTE010000024         1752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_412980, whole genome shotgun sequence.
ACCESSION   DATBTE010000024 DATBTE010000000
VERSION     DATBTE010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412980"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..134
                     /locus_tag="VIG33_00835"
     tRNA            59..134
                     /locus_tag="VIG33_00835"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:92..94,aa:Asn,seq:gtt)
     gene            284..1036
                     /locus_tag="VIG33_00840"
     CDS             284..1036
                     /locus_tag="VIG33_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269405.1"
                     /translation="MKIVTWNCNGALRKKTHEIDRLDADLVIVQECENPAESTEPYIT
                     WAGDYLWIGTSKSKGIGIFPKKGNRVKGLDWNREFVLSGLESKSNSLRWTTKDLKLFL
                     PFVLNDAITILSCWTKGSESEVFGYMGQFWKYLQIHRNELKNENTLILGDFNSNKIWD
                     KADRWWNHSDVIAELNEIGLESIYHHQHKEEQGEETIPTFFLHRNTSKPYHIDYVFAS
                     SDLLRMSRLEVGTFDKWLHISDHMPLSVEISS"
     gene            1368..>1752
                     /locus_tag="VIG33_00845"
     CDS             1368..>1752
                     /locus_tag="VIG33_00845"
                     /inference="COORDINATES: protein motif:HMM:NF025696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY8269406.1"
                     /translation="MELPKFNETFLPILEVLKDGEIIKGRDLVRLVEDRFYSNLPQDL
                     LEQITKSGDRLIENRIAWGKSYLKKGGLVHYPQRGYVQITEKGKSAKPENVSLNDIQS
                     NVITFYEPESKSAPDTTIVVNASPQD"
BASE COUNT          567 a          309 c          378 g          498 t
ORIGIN      
        1 cgaaccgtgc ggtcgggagt tcgaatctct ccgggcgcgc catactgaaa tagataattc
       61 ccctgtagct cagtcggtag agcggatgac tgttaatcat taggtcggcg gttcgagccc
      121 gtccggggga gccaaaaaaa tcaaggagtt accgttttcg gtagctcctt ttttttgtct
      181 gaaatttaag aagcggtcat ttggtctgga caatggtcat tactgtaatt tatttttgac
      241 cgcaacgatt gacgatcaaa acaatacttc actaaaagat aacatgaaaa ttgttacatg
      301 gaattgtaat ggcgcgttaa ggaaaaagac tcatgaaatt gatcgtcttg atgctgattt
      361 agtgatagtc caagaatgtg aaaatccagc ggaatcgaca gagccctata ttacatgggc
      421 cggtgattat ttatggattg gaacttcaaa aagtaaaggt attggtatat ttcccaaaaa
      481 aggaaataga gttaaaggct tagattggaa tagagagttt gttctttctg gactagaaag
      541 caaaagtaac tcacttcgct ggactacaaa ggatttaaag ctttttcttc ccttcgtttt
      601 aaatgatgca ataacaattc taagctgctg gactaagggt agtgaatcgg aagtgtttgg
      661 ctacatgggc cagttttgga aatatcttca gattcaccgc aatgaactta aaaacgaaaa
      721 cacgctaata cttggcgatt ttaacagtaa caaaatctgg gataaagcgg atagatggtg
      781 gaatcattcg gatgtcatag ccgaactgaa tgaaatcgga ttagaaagca tatatcacca
      841 ccagcacaaa gaagagcaag gcgaagaaac tatacccacc ttttttcttc atcgaaacac
      901 gagtaagcca tatcatattg attatgtttt tgcttccagt gatttactca ggatgagcag
      961 attggaagtt ggcacttttg ataaatggtt acatatcagc gatcatatgc ccttatccgt
     1021 ggaaatcagc agttagctat aaaaaatata aaacgctgaa cttgatctga cagtttcaaa
     1081 acattcgttt agatttagcc tgatattgac ggcctcagtt cgaccatgag cggacgttga
     1141 ataatgccga agtgactgat gctttattga atttattttc gaggaaaaaa tgtcgtttta
     1201 gcttgtcatt gtgcccgatt accctacact cagatcaata aaaacaatta actagatagc
     1261 tttatgagaa atatacgaca aatcagggtt tttataagaa attcgatgtc gtatatatta
     1321 cgttatgtat ttagttttac taatcaaact gcaagaacgg gaaattaatg gaattaccga
     1381 agtttaatga aacctttcta ccaatattgg aagtgctgaa agatggtgaa atcatcaagg
     1441 gccgagattt ggttcggctt gtagaggatc gattttattc taacttaccc caggatctgc
     1501 tggagcagat taccaaaagt ggggatcgtt taatcgaaaa tcgaattgct tggggaaagt
     1561 cctatctgaa gaaaggcggg ttagttcact atccacaacg aggctatgtt cagatcaccg
     1621 agaaggggaa atctgccaag ccagaaaatg tatctctcaa tgacattcaa tcgaatgtca
     1681 ttacttttta tgaacccgaa agcaagtctg caccagatac aacaatcgta gtgaacgcaa
     1741 gcccacagga tt
//
LOCUS       DATBTE010000025         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1574392, whole genome shotgun sequence.
ACCESSION   DATBTE010000025 DATBTE010000000
VERSION     DATBTE010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574392"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VIG33_00850"
     CDS             <1..295
                     /locus_tag="VIG33_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269407.1"
                     /translation="RPFPHHFKQYSSLRIVHFPIQTQKSQRKTGKRPQTFAPGTAPNA
                     KKNTFQDTNFLDKSEVVTESSLNTNPRKAPRQNILLELRNPLLVKTTLGTFFA"
     gene            complement(264..>2192)
                     /locus_tag="VIG33_00855"
     CDS             complement(264..>2192)
                     /locus_tag="VIG33_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY8269408.1"
                     /translation="DKCLRGDDAKDWKGISEKVGKTFARMNPVVGRSGRLTGQGGITQ
                     FKFPVHPNIMICTDVLKEGVDLHLFCDEVVHYGVAWTSGDLEQRIGRVDRFGSMISRK
                     IERHRGATEGALPRLQVEFPYLDGTLDKYQVDRVVKEKIKSDLRKDLGKKEDEIGIIS
                     LDELDAEELAPGISMRGTVLEPPLFYPVPDDTDSSSVVAPIAKAGLVSPQLERWLSNA
                     NQNTIALEVKHVPYLNSMRVRRNRVSRKMGEGNETNLLKISSLNHKSGKNIDVEEEFL
                     VPIELAHFVDLSTVMRETEESQGLTQFEKIDGFIFCPQWRTLVRDIRFNSPFEGEVER
                     CQTVLLERIGSFWLLRTPVIHRDGYDARWLATQNSGRTWGYLVAELDIIWFVVFVLHI
                     DGIHNPDFLTSLAERVGRIGDRLQHLYTASDESDNWNYRSKKYFPNLAGWKKGTLATL
                     VDKKEKIMTFMSDELGQLGQLIFRVQTWFHIAFEDVLASLYGEIPQEAWSLAISPVTV
                     LEDGTLHLKAMGAERFMLQAFLQLNDQSNKVQVFPVPRVIWELVATPHVIGSKPTIAP
                     SNWDVLPHNNLEGWAGDANGKCHTYTGKDESYRYFVLYHSPAYWDACRDVVLDAWAGI
                     LDTMKGTNFMQKKCRELF"
BASE COUNT          623 a          607 c          397 g          565 t
ORIGIN      
        1 ccgcccgttt ccccatcact tcaaacagta ctcgagtttg cgcatcgttc atttccctat
       61 ccaaactcaa aaaagccaac gaaagaccgg aaaaagacca caaacttttg ctcccggaac
      121 agcaccgaat gcgaaaaaaa acacatttca agacactaat tttcttgata aatcggaagt
      181 cgttactgaa agcagcttaa acacaaaccc tcgcaaagca ccgaggcaaa acattctgct
      241 tgagcttaga aatcctcttc tagtcaaaac aactctcggc actttttttg catgaaattc
      301 gtccctttca tagtatcaag aattcccgcc caggcatcga gcaccacgtc acgacaagca
      361 tcccaataag caggagaatg atagagcacg aagtaccggt agctttcatc tttacctgta
      421 taagtatgac actttccatt cgcatcgcca gcccaaccct cgagattgtt atggggtaat
      481 acatcccaat ttgaaggagc aatcgtcggc ttcgatccta ttacatgagg cgttgctacc
      541 agttcccata taaccctggg aactggaaat acttgcactt tattactttg gtcatttaac
      601 tgaagaaaag cttgaagcat gaatctctct gcccccatgg cctttaaatg cagtgttcca
      661 tcctcaagaa cagtaactgg agatattgcc agactccagg cttcttgtgg aatttcccca
      721 tacaatgaag cgagaacatc ctcaaacgct atgtgaaacc acgtttgtac tctgaatatc
      781 aattgcccca actgccccaa ttcgtcactc ataaacgtca taatcttttc ttttttgtca
      841 acaagagtgg caagcgtacc cttcttccac cccgccaagt taggaaaata ctttttggac
      901 cgatagttcc aattatccga ttcatccgat gcggtataca gatgctgcaa tcgatctcca
      961 atcctcccaa cacgctctgc aagagaagtc aaaaaatccg ggttatggat accgtcaatg
     1021 tgtaatacga atacgacaaa ccagataata tccaattctg cgaccaagta tccccacgtc
     1081 cgtccggagt tctgcgtcgc aagccatctt gcgtcatatc catcgcggtg aatcaccggc
     1141 gtacgcaata gccagaatga gccaattcgt tctaacagaa ccgtttgaca tcgctcaact
     1201 tctccttcaa acggactgtt aaaccgaata tcacgcacta aagtgcgcca ttgcggacaa
     1261 aaaataaatc catctatctt ctcaaactgg gtcaatcctt gcgattcttc agtctcacgc
     1321 atcacagtcg acaagtctac aaagtgagcc aattcaatag gaaccagaaa ctcttcttcg
     1381 acatcaatgt tttttcccga cttatgattc agggatgaaa ttttgagcaa gttagtctca
     1441 ttaccctctc ccatctttcg agacactcga tttcgacgca ctcgcataga gttcagatag
     1501 ggaacatgct taacttccag ggcaatagta ttctggtttg cattactaag ccagcgctct
     1561 agctgcggtg aaactaatcc agcctttgca atcggagcaa ccacacttga agagtcagtg
     1621 tcatccggca cggggtagaa aagcgggggt tcgaggacag tccctctcat actaatcccc
     1681 ggagcaagtt cttcagcatc cagttcatcg agagaaatga tccctatttc atcctctttc
     1741 ttaccaagat ccttgcgcag atcgctctta atcttctcct ttacgacacg atcgacctgg
     1801 tacttgtcca gagtaccatc aagataagga aattcaactt gcaagcgagg caatgctcct
     1861 tctgtcgcgc ctctatgcct ctcgatcttc cgacttatca tgctgccaaa tcggtccacg
     1921 cgtccaatgc gctgctccag atcacccgaa gtccaagcaa caccgtagtg cacaacctca
     1981 tcgcaaaaaa ggtgcaggtc aaccccttct ttcaaaacat ccgtacagat cattatattg
     2041 gggtgtaccg ggaacttaaa ctgtgtaata cccccttgac cagtcaagcg ccctgatcgg
     2101 ccaactactg gattcatcct tgcaaaggtc tttccaacct tttcgctgat gcctttccaa
     2161 tctttagcat catcgccgcg cagacattta tc
//
LOCUS       DATBTE010000026         8003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1710764, whole genome shotgun sequence.
ACCESSION   DATBTE010000026 DATBTE010000000
VERSION     DATBTE010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8003
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710764"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VIG33_00860"
     CDS             <1..180
                     /locus_tag="VIG33_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269409.1"
                     /translation="YADAECYHSLVKILVEDNKLTLAEDITAKAVRDYPHLRKDLYEL
                     LEPKDQSKIPLHHKS"
     gene            complement(177..1364)
                     /locus_tag="VIG33_00865"
     CDS             complement(177..1364)
                     /locus_tag="VIG33_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269410.1"
                     /translation="MGQSRRKFLQLTGLTFSSVLADVFFRRMEALAFSASKNRALRRN
                     PPWVALEGGDVNSILLRADGLIFACGKNTYGQLGINSTSSKSSFVQATDISNIVAVAS
                     EYSHTVALRADGLIFAVGDNFNGQLGINSVVSKSVFTQALGISNAVAVACGYTQTTAL
                     RSDGLVFTCGNNSFGQLGVNSITNKSVFTQAVGISNVVAINGRNRHTVALRSDGLIFA
                     CGYNSLGQLGIGLTSNKSIFTQASAISNAVAVACGDNHTVALRSDGAVFATGDNFFGQ
                     LGLNSGISESVFTQVSGISNAVAVACGYAHTVVLRSDGSVFAVGNNSVGQLGDNSIVN
                     KSVFTQAVGISNAVAVACGYTHTLALRSDGAVFACGNNQNGQLGNSTTIHKSSFIQVA
                     IKS"
     gene            complement(1366..2547)
                     /locus_tag="VIG33_00870"
     CDS             complement(1366..2547)
                     /locus_tag="VIG33_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269411.1"
                     /translation="MSSRRRFLQFTGTILGSILANSFFMRMEAMAFFPAKGRGRRNNV
                     PWGGGRCGANFTLLLRPDGLVFAAGENQHGQLGDNSINDKSAFTQALGISKITAVAGG
                     YTHALALRSDGFVFACGANASGQLGVNSVINRSTFVQALGISNAVAIAAGHNHSVALR
                     SDGLIFSSGNNSKGQLGDNTIVGKSTFVQAFGISNVVAVACGYFHTIALRADGFIFAC
                     GLNASGQLGDNSLIDKSTFVQALGVSDVISVAGAFTHGVALRSDGLVFAAGYNASGQL
                     GDNTTLNKSVYVQAQGVSNAVAIAAGQDYTAALRSDGLVFATGYNSSGQLGDNTVGAK
                     STYVQAQGISNAVAIAGGYSHTVALRSDNRFFACGYNLRGQLGDHSILQRSKFKPVVG
                     P"
     gene            complement(2567..5116)
                     /locus_tag="VIG33_00875"
     CDS             complement(2567..5116)
                     /locus_tag="VIG33_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269412.1"
                     /translation="MRLRKFVIFLILTSAAWLTSMAAASPFTFNLDGVISDSGNLPLE
                     APAVTFRVEVKNPAEDCLLYREDFSVSMTGSKGYFSLVVGKNANLVPGGAALDKIFSN
                     RATAIAGQSCSYTPASASDARKVIVSFNDGTGSQVFSTQEIQSVPFALQAQAVNGYGT
                     GDLLKVDSSVQQGTHANSDLSQAQYDEFWRLVKNPLAAYLPVTGDVSEVSGNNKLSSL
                     LGQALPTGPATNGQALVSNGTAWVLQSLSSGSVASVSGTAPLVVSGTAMAPVVSLPAA
                     TTLADGYLTASDWNTFNSKLSSGLTSGKILVGNASGGAAEVTLSGDATLSNSGALTLK
                     GIGTAGTYGSVSSIPVITTDAQGRVTGVTSAAPVDATKLPLAGGTMTGAINTGGFDIA
                     NTGNITMATSKILGLSSNPTDPGGLSAGQVWYNSTSNVIKYYDGSAVQSLGAAGAGLS
                     SLNGLTSATQTFAIGTSGISPAFSSATSTHTLNIPLASTAAVTAGLISKTDYDAFNAK
                     LSVVTDTAALTATKIWIGNASGKAQEFALSGDVTMTSGGVVTVEKTQTATASKILQLD
                     ASSVAATKGIDISGAGSGKISLRYPSTATNTTLTLPTSVGSANQFLQTDGTGNLLWAA
                     PDASSMTGTLGVVNGGTGLTSFANYSVVTSNASGGLQSTAGSVVGSSLVHTITGPGFA
                     NDLLFGMANSPANYVVVTGASSGASPTIAAAGSDANVSLSLAGKGDGHVKINSSYVER
                     TPSSVNISSSYTIPTGYNIVRVNLNGNATLTLPQPPSGSNQVFSLTVKVQQDATGHWT
                     LSWAGGGTSTIKWDGGTLPVPAMDPNTETIYQFLYFDTENIWYASQTWKEQ"
     gene            5475..5903
                     /locus_tag="VIG33_00880"
     CDS             5475..5903
                     /locus_tag="VIG33_00880"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY8269413.1"
                     /translation="MKKGIFKSILFITSLLLSLEALSKIEKSAEKKVESKSDSKSKSK
                     SAKALDMNAVRLAVHFHLQEIQKCYTDLIIEGMATKGKVTATWDIDDKGSVQNLFIKE
                     NTSKDPALEGCVTERVVNWTFPAAETGKTFPVTYPFQFGN"
     gene            5978..7630
                     /gene="hutU"
                     /locus_tag="VIG33_00885"
     CDS             5978..7630
                     /gene="hutU"
                     /locus_tag="VIG33_00885"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY8269414.1"
                     /translation="MAQSRVVRAPTGNKLACKGWLQEAAYRMIQNNLDPMVAERPEDL
                     VVYGGIGKAARNWESFDKILEALKNLENNETLLVQSGKPIGILKTTEDAPRVLLANSN
                     LVPKWATWEVFNELDKKGLMMYGQMTAGSWIYIGTQGIVQGTYETFVEAGRQHFGGSL
                     KGRAILTAGLGGMGGAQPLAGVFAGACVLAVEIDPTRIQKRLETKYVDEVATDLEDAL
                     ARIRKYTQAGEAKSVALQGNMATVIWQLLEKNYTPDLLTDQTSAHDPLVGYIPEGYTV
                     ETAADFRKRDQKAYLAAAYASIAKHVRGMLAMKDKGAVTFDYGNNLRARAQEAGVTNA
                     FDYPGFVPAYIRPLFCKGSGPFRWVALSGDPNDIKVTDQALRELFPHKKDLLRWLDMA
                     KERIAFQGLPARICWLEYGERAKAGLMFNKLVAEGKVKAPLVIGRDHLDCGSVASPNR
                     ETEAMKDGSDAVSDWALLNALVNTACGATWVSLHHGGGVGMGYSQHAGQVIVADGTPA
                     AAARLERVLTADPAMGVFRHVDAGYEEAIEVARERGVKSLWI"
     gene            complement(7672..>8003)
                     /locus_tag="VIG33_00890"
     CDS             complement(7672..>8003)
                     /locus_tag="VIG33_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269415.1"
                     /translation="GSIRRLHLIPAENTNARNNTLELRYFSLDEEGFPVRITLPSAQS
                     LNPKPEFLKSLKNQGTTIYHETRETQTLKDGTTLSLAMINDKVYEFRIFQDGKNFSCK
                     EFNCLCR"
BASE COUNT         2182 a         1966 c         1922 g         1933 t
ORIGIN      
        1 tatgcggatg ctgaatgcta tcactctctc gtaaagatat tagtggaaga caataagctc
       61 acgctggctg aggacatcac tgctaaagcc gttcgcgact atccccatct gcggaaagat
      121 ctttatgagc tcctagagcc caaagaccaa agcaaaatcc ctctccatca caagagttag
      181 gacttaatgg ccacttgaat aaaactggat ttatgaatcg ttgtactgtt acctagctgg
      241 ccattttgat tattcccaca ggcaaaaacc gcaccgtctg aacgcaaggc aagggtatga
      301 gtgtatccac acgccaccgc caccgcattt gaaatgccca ccgcctgggt aaaaaccgat
      361 ttgttgacga tagagttatc cccaagttga cccacagaat tatttccaac cgcgaagact
      421 gaaccatctg accgcaaaac aactgtatgg gcatatccac aagcgactgc caccgcattt
      481 gaaatacccg atacctgagt aaaaactgat tcactaattc cagaattaag tccaagttgg
      541 ccaaaaaaat tatctccagt cgcaaagaca gcaccatccg atcgcaaagc aacagtgtga
      601 ttgtctccgc aagccaccgc cactgcattg gaaatggccg acgcctgggt gaatattgat
      661 ttattggagg tcaagccaat cccaagttgg ccaagactat tatagccgca agcaaagatc
      721 aaaccatcag accgtaaggc caccgtgtga cgatttcgac cattaatcgc cactacattt
      781 gaaataccca ctgcctgggt aaaaactgat ttgttagtga ttgagttaac cccaagttga
      841 ccaaaagaat tattgccgca agtgaaaacc aaaccatcgg agcgtaaagc agtggtctga
      901 gtataaccgc aagccaccgc cactgcattt gagatgccta aagcctgggt aaaaactgat
      961 ttgctgacga cagagttgat tccaagctgt ccattaaaat tatcccccac cgcgaagatc
     1021 aaaccatcgg ctcgcaaagc aaccgtatga gaatattcgc tagccaccgc cactatattt
     1081 gaaatatcgg tggcctgcac aaaactcgat ttgctgctcg tcgagttgat tcccagttga
     1141 ccataagtat ttttgccgca tgcgaagatc aagccatcgg ctcgcagcaa gatgctattg
     1201 acatctccac cctctaaggc aacccacgga ggattacggc ggagagctcg atttttcgaa
     1261 gcggaaaaag ctagagcctc catacgccta aaaaaaacat ccgccaatac agagctaaag
     1321 gtaagaccgg tcagttgaag gaattttctc cgcgactgac ccatcttatg gtcccaccac
     1381 tggcttgaat ttggatcttt gaagaatcga atgatctccc agctgccccc gcaaattata
     1441 accacaagca aaaaatcggt tgtccgaacg aagggctacc gtatggctat atccgccagc
     1501 aattgctacg gcattggaaa taccttgggc ctgcacataa gttgatttag caccaactgt
     1561 attgtctcca agttgtccag aggaattata acccgttgca aagaccaaac catcggagcg
     1621 cagcgctgcc gtgtaatcct gtccagcagc gatggcaaca gcattggaaa cgccctgcgc
     1681 ttgcacataa actgatttgt tgagtgttgt attatccccc agttgccccg aagcattgta
     1741 cccagctgca aaaaccaatc catccgatcg taacgccact ccatgagtaa acgcgcctgc
     1801 gaccgaaatc acatctgaaa cacccaatgc ttggacaaaa gttgatttat caatcaggga
     1861 attatcacca agctgaccac tggcattaag tccgcaggca aagataaacc catcggctcg
     1921 caaagcaatc gtatgaaagt aaccacaagc cactgctacg acatttgaaa ttccgaaagc
     1981 ttggacaaaa gttgatttac caacgattgt attatcccca agctgacctt tactattatt
     2041 tccagaagaa aaaataagac catccgatcg caaggctacg gaatgattat gtcctgcagc
     2101 gatcgccacc gcattagaaa ttcccagagc ttgtacaaaa gtagatctat ttatcaccga
     2161 attaactccg agctgaccac tggcatttgc tccacaagca aagacaaaac catcagatcg
     2221 tagggctaag gcatgcgtgt atccgccggc taccgctgtt attttagaaa tgcccagagc
     2281 ttgcgtaaag gcgctcttgt cattgattga attatctcca agctggccgt gttgattttc
     2341 tcctgcggca aagaccaatc cgtcaggacg aagtaggagg gtaaagttag ccccacatct
     2401 gccgcctccc caaggaacat tgttacgacg acctcgccct ttggcgggga aaaatgccat
     2461 cgcttccata cgcataaaaa aagaattcgc caaaatagaa ccaaggatcg tgcctgtgaa
     2521 ttgaagaaat cgcctgcgtg aactcattcc accctctagt gagttactac tgctctttcc
     2581 aagtttgcga tgcataccag atattttcag tatcaaaata aaggaactgg taaatagtct
     2641 cggtgtttgg atccatcgct ggcactggca aggtaccgcc gtcccatttg attgtggagg
     2701 tccctccgcc tgcccagctc aaagtccaat gtcccgtggc atcttgttgc acttttacgg
     2761 ttaatgaaaa aacttgattc gagccagatg gaggctgtgg caaggtcaga gtggcatttc
     2821 cattcaaatt cactcgcaca atattatatc ctgttggaat ggtatagctc gacgaaatat
     2881 taacagagga aggagtccgc tctacataag aagagttgat cttcacatgg ccgtctcctt
     2941 ttccagccaa ggagagggat acattggcat cagaaccggc agcggcaatc gttggcgaag
     3001 ctccgctgga tgcgcccgta acgacgacat agttcgctgg agagttcgcc attccaaata
     3061 acaaatcatt ggcaaaaccg gggcccgtga tcgtatgaac cagagaactc ccaaccacgc
     3121 ttcccgctgt cgactgcaaa ccacctgaag cattcgaagt gaccaccgaa taattggcaa
     3181 aggaagtaag cccggtcccc ccgttaacaa ctcctagagt tcccgtcatt gaagatgcgt
     3241 caggagctgc ccacaataaa ttcccggtgc catcagtttg caaaaactga ttcgctgacc
     3301 cgacagaggt tggcagagtt aaagtggtat tggtggctgt gcttggatag cgcagactta
     3361 tttttccact cccagctccg ctaatatcaa ttccttttgt cgccgccacg gaagaggcat
     3421 ctaattgcag gattttacta gctgtggctg tttgggtttt ctccactgtg accacgcctc
     3481 ctgaagtcat cgtgacatcg cctgaaagag caaattcttg cgccttacca ctggcgtttc
     3541 cgatccagat ttttgttgcg gtgagtgctg cggtgtcggt gacaacgctg agttttgcat
     3601 taaaagcatc gtagtctgtt ttactaatca gacccgctgt gacagcagct gtggaggcca
     3661 gggggatatt cagggtgtgt gtgcttgtgg cggagctaaa agcgggggag atgccactgg
     3721 tgccaatagc gaaagtttgg gttgcggacg ttaagccatt taaactactc aggccggcgc
     3781 ctgcggctcc taaagactgg acagcgcttc catcgtaata cttaatcaca ttcgaggttg
     3841 agttgtacca aacttgaccg gcgctgaggc cgccagggtc tgtgggattg ctcgaaagcc
     3901 ccaaaatttt acttgtggcc atggtgatat tgccggtgtt agcaatatca aagccccccg
     3961 tatttatcgc acccgtcatg gtgcccccag caagcgggag ctttgtggca tctactgggg
     4021 ctgcgctcgt cacccccgtc acacgacctt gagcatcggt ggtaataaca ggaattgaac
     4081 tgactgaacc ataggtgcca gcggtgccga tgccttttaa agtcaaagct ccggagttag
     4141 aaagtgtagc atctcctgag agcgtcactt ccgccgctcc ccctgaggcg ttgcccacaa
     4201 gaatttttcc tgaagttaaa cctgaagaaa gtttggagtt aaaagtattc caatcactgg
     4261 cagttaaata tccatccgct aaagtggtcg ccgcaggcag acttacaact ggagccatgg
     4321 ctgtgccgct gaccaccagc ggagctgtgc cgctgacact tgcgacggag cctgaactca
     4381 gagattgcag aacccaggcc gtgccattag acactaaagc ctggccattg gtggcaggtc
     4441 ccgtcggcag agcttggccc aaaagagaac ttaatttatt gttgccactc acttcactga
     4501 catcgccggt gactggcaaa taagcagcca ggggattttt cactaaacgc cagaactcgt
     4561 catattgagc ttggcttaaa tcactattgg catgggttcc ttgctggaca ctggaatcga
     4621 cttttaaaag gtctccggtg ccataaccat tcaccgcttg agcttgcaaa gcaaaaggca
     4681 cggattgaat ttcctgagtg ctaaacacct gggaaccggt gccatcgtta aaggacacaa
     4741 tcactttccg agcgtcggaa gccgaggccg gcgtataaga gcaactctgg ccggcaatgg
     4801 cagtggcgcg gttagagaag attttatcca gagctgctcc cccggggaca agattcgcgt
     4861 tttttcccac caccagggaa aagtagccct tggatccggt catggaaacg gagaagtcct
     4921 cgcgatataa aaggcagtct tcagcgggat ttttaacctc cacccggaag gtgaccgctg
     4981 gcgcttccag aggaaggttc ccgctgtcac tgatgacccc atcgaggtta aaggtgaagg
     5041 gcgatgccgc agccatggac gtaagccatg ccgcactggt caggatcagg aagatcacaa
     5101 actttcttaa cctcattaaa ccgactcctt gaccacttat cggttttcta aaatttattt
     5161 aaaggaactt gtttagcaac tggtcggaaa aatctttatt agaatgcttc agaatgggat
     5221 ttggtctcag tctgcctcaa gagtgttgcg cgttgatatg ctccaattat ggactctgtt
     5281 ttcaaacttt gctttatcgc ctcaaaagag tttttagaaa attctcgact ggaagagttt
     5341 ccattggatc ttggtctggc ataaagttag ttttttcgta aaaacatcgg tcaaaaaact
     5401 cctcttcacc ggccccactt tgcaataggg gtttcgaggg gggtgatata tacttgtgta
     5461 taaggaggtg ctatatgaaa aagggaatat tcaaaagcat ccttttcatc acgagcttgc
     5521 tactcagctt agaggccctc tccaaaatag aaaaaagtgc ggagaaaaaa gtagagtcaa
     5581 aatccgattc aaaatccaaa tcaaagtccg caaaagcttt agacatgaat gcggttcgcc
     5641 tcgctgtaca tttccatctc caagaaattc aaaagtgcta cacagactta atcattgaag
     5701 gcatggccac caaaggcaaa gtcacagcca cttgggatat cgacgacaaa ggcagcgtgc
     5761 aaaatttatt catcaaagaa aacacctcta aagatcccgc tcttgaaggc tgcgtcaccg
     5821 aacgagtcgt caattggacg ttccccgctg ccgaaaccgg aaaaaccttt cccgtcacct
     5881 atccatttca attcggcaac taacgccgcg cagtactaga aagtacggcg tacctttaag
     5941 gacttttctt cgttattttt gctgacggag gaattttatg gctcaatctc gcgtagttcg
     6001 tgcacccaca gggaataaac ttgcttgtaa aggctggctt caagaggcgg cttatcggat
     6061 gattcaaaat aacttggacc ctatggtggc ggaacgtccc gaggaccttg tggtttatgg
     6121 cggcatcgga aaagcagccc gtaactggga gagttttgat aaaattttgg aagcactcaa
     6181 aaatttagaa aataatgaga ctctgttggt gcaatccgga aagcctatcg gcattttgaa
     6241 aaccactgaa gatgctccgc gcgtgttgtt ggcgaactcc aatcttgttc cgaagtgggc
     6301 cacctgggaa gtcttcaacg aactcgataa aaagggtttg atgatgtacg gccagatgac
     6361 ggcgggatcc tggatttaca tcggcactca agggattgtt caagggactt atgaaacctt
     6421 cgttgaagcc ggccgccaac atttcggagg cagccttaaa ggacgcgcca ttcttaccgc
     6481 tggtctcggc ggaatgggcg gagcgcaacc tcttgccggc gtgtttgcag gagcatgcgt
     6541 gctggccgta gagattgatc ccacacggat tcaaaaacgt ttggaaacaa aatatgtcga
     6601 tgaagtggcg acggatctcg aggatgcttt ggctcgcatt cgtaaataca cgcaagcggg
     6661 tgaagcaaaa tccgtggccc ttcaaggaaa tatggctaca gtgatttggc agctcttaga
     6721 aaagaattat actcctgact tgttaactga tcaaacgtcg gcccatgatc ccttagtggg
     6781 atatattcct gaaggttaca ccgttgaaac cgctgcggat ttccgcaagc gagatcaaaa
     6841 ggcctacctc gcagccgctt atgcctcgat agcaaagcat gtccggggca tgctggcgat
     6901 gaaagacaaa ggagccgtga ctttcgacta tggtaataat cttcgcgctc gcgctcaaga
     6961 ggcgggagta accaacgcct ttgattatcc tggattcgtg cccgcttaca ttcgtccact
     7021 tttttgcaag gggagcggcc ccttccgttg ggtggctctt tctggcgatc ccaatgatat
     7081 caaagtcacg gatcaggcat tgcgagaact tttcccgcat aaaaaagatt tgctaagatg
     7141 gttggatatg gctaaagaac gcattgcttt tcaaggattg cccgcgcgca tttgctggct
     7201 ggagtatggc gagcgcgcca aggcaggttt gatgttcaat aaattggtgg ctgaaggtaa
     7261 agtgaaagct cccctcgtga ttggccgtga tcatttggat tgcggctcgg tagccagccc
     7321 gaatcgcgaa acggaggcga tgaaagatgg ctccgatgca gtcagcgatt gggccttgtt
     7381 aaatgcactt gtgaataccg cctgcggtgc cacctgggtg agtctacatc acggcggagg
     7441 cgtcggcatg ggctacagtc agcatgcggg acaagtcatc gtggccgatg gaacaccagc
     7501 cgccgccgct cgattggaaa gagttctcac ggcggatccc gccatgggag ttttccgtca
     7561 tgtagacgca ggttatgaag aggctattga agtcgcacgc gagcgtggtg ttaaaagtct
     7621 ctggatttag aatttgcaat cgaatgggac attaaaaggg gacattaaga attaacggca
     7681 aaggcagttg aattctttgc aggaaaagtt tttgccatct tggaagatgc ggaattcata
     7741 gactttgtcg ttaatcatgg ccagagacaa agtcgttccg tccttcagag tctgagtctc
     7801 tctcgtttca tgatatatcg ttgttccttg attcttaagg gacttcagga actcaggctt
     7861 tggattcaaa ctctgcgcag agggcaatgt gatccgtacc ggaaaacctt cttcatccag
     7921 actaaaatag cgaagttcaa gggtgttatt tcgagcgttt gtattctccg cagggatcaa
     7981 atggaggcgt cgtatagagc cat
//
LOCUS       DATBTE010000027        13869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2120760, whole genome shotgun sequence.
ACCESSION   DATBTE010000027 DATBTE010000000
VERSION     DATBTE010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 13869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13869
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2120760"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..937
                     /locus_tag="VIG33_00895"
     CDS             149..937
                     /locus_tag="VIG33_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269416.1"
                     /translation="MNKNDSIDNTMLRVITAFGDSDQYCGDILKNVRLVATEPQGSAV
                     DSIDFHNQNHSVLQNAAKIGALVEGKRDLVLSDIRLKPVTALSADIIVASLELTFKKS
                     NVLGPPFIMRWLPIYATVKNNVVDTCSTSNNSAVALKIRLCEIANDGFNHYDVATDSC
                     ILNQDVKWTNGVTSFEAACESGWTPAVSQVNGAPELTVCRAETPPFVSLAPRAYLRTG
                     VESGATSSWFAAMDAARTKCIFTYVAGINSSLHPPKIKCARSVL"
     gene            934..1830
                     /locus_tag="VIG33_00900"
     CDS             934..1830
                     /locus_tag="VIG33_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY8269417.1"
                     /translation="MKVRGFSLVEMLISVAVISCLVLVLTFFSQNAFRIFANMEKTKS
                     IAEVMEQLRFTLAVGEQCSLNFKDLPLSVADTAGVPLQSISTFDNNTKLKNSDIVVVS
                     AGSNLTSAQSILLIPQSQLNIGLISANLQVAFQAGNNANVPAILRSLPILVRVQGGKV
                     QECWVKRDSSHVAGGLLCQALTAQALNSVGSTQCELRNGRWFIGTSTQASCPAGALLP
                     AQANSDANCAVDMPSSFVDTFPKSNISVVSGGGTDQWPMARSPVLLSLDAPSSTCYCD
                     AAADLSSAVRATFACKILCLVP"
     gene            complement(1835..2467)
                     /locus_tag="VIG33_00905"
     CDS             complement(1835..2467)
                     /locus_tag="VIG33_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269418.1"
                     /translation="MGKNQQQGNIIILALPLVISIAILMGVFISWLDFHMKLQARDRV
                     LEDASHLSTMLKMVFSNRAACKRNLSAAAFGTSRSSLGVSKNLSVLKSSFMGATPEVW
                     FSSGQNYHGLLINDVKFSVARKLPALENSYIADLSILYRNPLTQTDRELTLPFYFITD
                     STGNTLQDCLATSYIDPHSQITTEDRICMVQHGSGNYIYLPKNQTCALKN"
     gene            complement(2618..3742)
                     /gene="rodA"
                     /locus_tag="VIG33_00910"
     CDS             complement(2618..3742)
                     /gene="rodA"
                     /locus_tag="VIG33_00910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY8269419.1"
                     /translation="MEFSIQVEKRTIFKRLDLNFLLVIFALNVIGLINLYSATHGPHS
                     RDVEPLFISQFVWLGIGWSVFFAFTLLDYALVARLAIGVFVLNLGALLYTTFFGTVAL
                     GAQRWIDLGFFRYQPSETMKLALIVLMAKILATRNSAGLGMGLKELAVPLFVLLLPFV
                     LVVEQPDLGTGMMLAAIGGSMILFTKVRKSILAVVITAVVITAPLAWQFGLREYQKNR
                     ILTFMSPTNDPRGTGYNSIQSKIAVGSGRFLGKGFRKGTQSQLEFLPERHTDFIYSVL
                     SEEHGFVGSMMTMGLFCMLFILGLRIAMNSRDKFGVLLSIGVLCYVFWHMFVNIGMVI
                     GLLPIVGVPLPLLSYGGSSMLTTMAGLGLISSVAYRRYLF"
     gene            complement(3745..5733)
                     /gene="mrdA"
                     /locus_tag="VIG33_00915"
     CDS             complement(3745..5733)
                     /gene="mrdA"
                     /locus_tag="VIG33_00915"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY8269420.1"
                     /translation="MSDYVNNPDEAKEYQPRYRLFYIAVACTFIIFSARLWYLQIITG
                     NELREFSEKNRVKQNKISAPRGLMLDRNGKVLVENLPGFEAILSPQYIENLETMANTV
                     APILGMEAEKIVQKVQKSRKQNGPFSQVRLKENLSREEVFRLKRIRLDIPGLEIRESI
                     VRYYPLKEDGAQVFGYVAEISKRQIPLMNDLYKNVFLFEQGDIIGKSGLEETLEKDIR
                     GKDGLSFIQVDAHGRETVTTTPNIYGEQIRDQEPVHGNNAVLTIDRDVQEAAYKSMID
                     NKRIGAAFVMKSNGEVLAWVSTPSFDPNDFSTGFSAPTWSKLINDPFKPLRNKVIQDY
                     TYPGSTFKPFVAVAALSEKVVGPTTLVAAPGVFMFGRRPYHDSNKNGYGMITILEAIE
                     RSSNIFFFKMGIALGVDKMFAYINPMGIGQKTGVELAREVAGTMPNSEWKKAAMGEEW
                     QAGENLSTAIGQGFVQVTPIQMAVAYNAIGTEGKVVKPFIIRKIIDQEGKILKENFPQ
                     VVRDLTEEQKTGVKVLPATFKIVKEGMRRVANGEHGTARFWKVPGVPMAGKTGTAQVM
                     NFSADQIYASCMARPIHMRHHGWFVAWAPADHPEITVAILAEHACHGNTGAAPIARDI
                     VQTYFQKYYPERIEAGLKELHLKKAAKINLPATIMDGE"
     gene            complement(5730..6266)
                     /locus_tag="VIG33_00920"
     CDS             complement(5730..6266)
                     /locus_tag="VIG33_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269421.1"
                     /translation="MKIRWNKVLNFFLLLLVLMVLAAFQSTFWYQLFGTVPAPMLWLC
                     LIVYVALYRKLYGALFIIYVMSLALMGFTVMPMKMLLICLLLVYLILYSIRSRVFWEG
                     PAYYTIMCCVASLSYHLIYFFSSLAIERNSAPIEFWDRIIQLGLTPAFCLPMFWVLMK
                     IDKLTLDEMTPESRGYEL"
     gene            complement(6263..7207)
                     /gene="mreC"
                     /locus_tag="VIG33_00925"
     CDS             complement(6263..7207)
                     /gene="mreC"
                     /locus_tag="VIG33_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY8269422.1"
                     /translation="MNFFNIDLKKLIMIVIVLALPLVSINMQQKPQESNWLSRPFSLI
                     ASLAEEAFYGFSAGVRGTTAMYLDLINIKKSNLELTSKNNELLTRMSEVVEMKNENDR
                     LRALLDFRQSTKLELLSAQVIALDLFADHQTVTINKGTKHGLKAGQAVITVQGVLGYI
                     YRPEALTSNVMLITDRYSVVDGVVQRTRAHGIVEGKNPGTCQLKYVEKTEDVKKGDLV
                     VTGGLDNIFPKGFPVAIVESVERKNFSVSLKVDLKPVVDPYKVEEVFIVTNALNTDLS
                     DRLPPQASEPTPLPPGMGVGVGAGLGVLPAPPAAEKTR"
     gene            7340..8827
                     /locus_tag="VIG33_00930"
     CDS             7340..8827
                     /locus_tag="VIG33_00930"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY8269423.1"
                     /translation="MSHSMADRLRKQVSAKNFTAILLFGAIILVFVFFGVSGRLGGGG
                     LGAAAQVNDALISVANLQAAENNLRQIYGEVFGNDAFRLRAEALRGLVDRELMSQAAR
                     KNGIVVADSEIAHTITKDIPVFQTNGQFQKDYYLRYLEMTHQVPKEFENSIRQQQEAL
                     RVRHLFDIASGMTALETAKMADLKAYKMNVQFVHIDHNQLAKSFTVSSAEAEKALVNA
                     EFLKKAQDYFTKNQTDYAQEEEIHAQHILLQIKPGASEEDSKKVLAKIQDLQKKAAAG
                     DFGKLAAANSEDPGSKVKNGDLGFFGRGKMVKEFEEVAFSAPIGKVMGPVKTPFGYHL
                     IKVTEKKAAQMPDFEKQKVQVAQKVLADEKTATGVKALEEAVGKGDESALELALKNIN
                     AKWEETGSFDLSQESAPKINSVLANNALAELTLEQKLLKRIVREGDEKYILKLKEAKM
                     EAPIPLDSMTKTMTESYRANMLFGNWLNEFKKVSKVEVNNQLLKP"
     gene            8824..9426
                     /locus_tag="VIG33_00935"
     CDS             8824..9426
                     /locus_tag="VIG33_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269424.1"
                     /translation="MNLSLHRSHKMAVNITRPGFDARAFVFDVALGLRLSADLPLELQ
                     VDPLSGMLINLVQMDELLQELEQMWGAKSWNSLSVLLEESRKFLVGRVPQEPGAHPLT
                     LYEIALTEKRGFWLKWQVQNAGRFFQGYEEIRELDTKLFRFRSEYLLHEDGWEQQRLK
                     YLKVISTNDLFSEKIFQDNPGLHSLEIENLTTSKKWSFFL"
     gene            9572..9982
                     /locus_tag="VIG33_00940"
     CDS             9572..9982
                     /locus_tag="VIG33_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HEY8269425.1"
                     /translation="MAMREPMQDSDTAHCIKSCLSNKRICLETLHYCLTQKMTHFQGR
                     HIALLGMCADACELAAKMMIADIQFHRQSCVVAFELCLACAEECEKYMEDTAMKRCAD
                     SCRKCAEFCRGMMGTTVEITSAAGQDRRPPGISR"
     gene            complement(9933..10328)
                     /locus_tag="VIG33_00945"
     CDS             complement(9933..10328)
                     /locus_tag="VIG33_00945"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEY8269426.1"
                     /translation="MFTELLKYAIEGQKNSYAPYSGKNIGAAVLMDNGKIYGGCNVEN
                     ASYGGTICAERTAIFKAISEGAKKIEEVLVVSSEKNPWPPCGFCRQVIAEFASPETKI
                     HLSNPQGHSRTLKFIDLFPEAFGPAQLQK"
     gene            complement(10336..10908)
                     /locus_tag="VIG33_00950"
     CDS             complement(10336..10908)
                     /locus_tag="VIG33_00950"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY8269427.1"
                     /translation="MKKIVLASTSQYRQELLRQLGLPFEARKPLIDEESEKDPSLSPL
                     KLAEKLAFLKAQSLKGADLVVIGGDQLVAHSGIILGKPHTRERAIEQLLRMQGQIHEL
                     VTAICIFDGESCTPYADITRLHMKSLSQEQIERYVDLDQPWDCAGAYKIEQHGMMLFD
                     KIESQDFSAIQGLPLLALSRILNNSGFSIP"
     gene            11029..11784
                     /locus_tag="VIG33_00955"
     CDS             11029..11784
                     /locus_tag="VIG33_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269428.1"
                     /translation="MKYLIFWAWGMFSFTCAAYGATVGCLSGEYLVSGHHRRGYVRSD
                     GTVVRSTDVKSYCKVLTKAYEYSKKRFKKGRPANWPHNIEKASSWTDEETERIVEVLE
                     ELPEVLLSKVIVGIYRLKRSKDYPNPASSSDGVIVIYDSSFDLSRNLGRIIAHELSHQ
                     NFKDLSKDEKQDYRRATGWRLKIESDRNVYWVGRKDGYVEEDGKISYEEDYSNNLEHY
                     LYDPDKLKRVTPSAYEWIKKHFGDGFQIKRQKR"
     gene            11781..12344
                     /locus_tag="VIG33_00960"
     CDS             11781..12344
                     /locus_tag="VIG33_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269429.1"
                     /translation="MKFKGILMTLFLSACVTNTTGSNRGSLNYDKVRSLVVGYSTESD
                     VLAVLGLPTGRTEEAGYYILNYDDSKTGFQRLSINFLLGSQKLSGILWIPREDEKEYS
                     LMQAKAGFKNADFKEIINRSTNPHAISPDEVSYIDEKHGVTIRYDRSQNIVEAIAMYD
                     VNVRLPAATEKKNQIPYTFGDEPAISK"
     gene            12375..13313
                     /gene="folE2"
                     /locus_tag="VIG33_00965"
     CDS             12375..13313
                     /gene="folE2"
                     /locus_tag="VIG33_00965"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEY8269430.1"
                     /translation="MDKQKLPDIAMETRTETRKDLYPPIDQVGMGNIEVPVLLSSDET
                     MLFRVPARVDAKVSLDRASSRGIHMSRLYLSVQEMLSSKALSYSLLEELSQEFLKTHE
                     DLSRRSFVTVKFEAPVQRKALKTQNKAWRTYPVALSVENFEGRAKIFAEILVTYSSTC
                     PASAALSRQLIQENFKQEFLSQDELSFADIHQWLGSTQGVIATPHAQRSKARVKVQFH
                     SASSSLSFSELIDVVEDAVQTPVQGAVKRQDEQEFALRNGQNLMFCEDASRRIKTSLD
                     LRPEISDYLIKVKHMESLHPHNAEALIAKGVSGGLS"
     gene            13447..13839
                     /locus_tag="VIG33_00970"
     CDS             13447..13839
                     /locus_tag="VIG33_00970"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY8269431.1"
                     /translation="MFTRKIVTAAVMAMAVNGTVFAVSHSDADNTKLNQRDAKNTKQS
                     ALTPDIQTKGSPQDVELTRLLRQELTNDKTLSTNAQNIKIITLDGIVTLRGPVESQEE
                     KQRIDALAKKTAGVKRVDNQLDVKSKAY"
BASE COUNT         3906 a         2918 c         3277 g         3768 t
ORIGIN      
        1 ttaaatgaga gctgataaat ccattactta tttcataaat aatcgtggca tctccttggt
       61 agaggtgatg gcttccgttg ccatttttgg catcgtagct ttgatgtttt tgcattgggt
      121 tcaattcttt agcctctcta caagcagtgt gaataagaat gacagtattg ataatacaat
      181 gttgcgggtc attactgctt ttggtgactc tgatcagtat tgtggagata tattaaaaaa
      241 cgttcgcctg gtcgccacgg agcctcaggg gtcagcggtg gactccattg attttcataa
      301 tcagaaccac agtgttttac agaatgctgc gaaaatcgga gccttggttg aaggcaagcg
      361 ggatttggtg ttgagcgata ttcggttgaa gcccgtcaca gcgctttctg ctgatataat
      421 tgtggccagt cttgagctca cttttaaaaa atccaatgtt ttgggtcctc cgtttataat
      481 gcgttggttg cccatttatg ccactgtgaa aaacaatgtt gtggatacgt gttctacctc
      541 taacaattcc gccgtggctt tgaagattcg actgtgtgaa atcgctaacg atggcttcaa
      601 tcattatgat gtggccacgg atagctgtat cctcaatcaa gatgtgaaat ggacgaatgg
      661 cgtaacttca tttgaggcag cctgtgaatc aggttggact cccgctgtct ctcaagtcaa
      721 tggagctcca gaattgacgg tttgtagagc ggagacaccg ccttttgtct ccttagctcc
      781 tcgtgcctat ctcagaactg gggtggagag tggagcaacc agttcatggt tcgcggccat
      841 ggatgccgcg cgaacaaaat gcatttttac ttatgtggca ggtataaact cttccttgca
      901 cccacctaaa atcaaatgtg cgaggtcggt attatgaaag tccggggttt tagtttagta
      961 gaaatgctga tttctgtggc tgtgatttct tgcctggttt tggttcttac ttttttttca
     1021 caaaatgctt ttagaatctt tgccaatatg gaaaaaacaa agtccattgc tgaagtgatg
     1081 gagcagttgc gctttacttt agcagtgggt gaacagtgca gcttaaactt taaagatttg
     1141 cccttgagtg tcgcagatac ggccggagtt ccccttcaga gtatttctac ttttgataac
     1201 aatacgaagt tgaaaaacag tgatattgtc gtcgtttctg cgggttcaaa tttaacttca
     1261 gcgcaaagca tccttttgat tcctcagagc caactgaata tcggtcttat ttctgcaaat
     1321 ttgcaagttg cttttcaggc tgggaataat gccaatgtgc ctgccatatt gagatcattg
     1381 cccatcttag tccgagtcca aggtggaaag gttcaagagt gttgggtcaa aagagattct
     1441 tcccatgtgg caggaggtct tctctgtcag gctctgacgg ctcaggcttt gaattctgtg
     1501 gggagcacgc aatgtgaact taggaatggt cggtggttta tcggcacttc aactcaggcg
     1561 agttgtcctg cgggagctct actgccagcg caggcaaact cagatgcgaa ctgtgctgtc
     1621 gacatgcctt cgagctttgt cgacactttc ccgaagtcca acatctcggt ggtgagtggc
     1681 ggcggaacag accaatggcc gatggctcgt tctccagttt tattgagcct cgatgccccc
     1741 agttccactt gttattgtga tgcggccgcc gatctttcat cggccgttcg tgctaccttt
     1801 gcttgtaaga tcctgtgctt ggttccctga agtattagtt ttttagagca caggtctggt
     1861 tttttggtaa gtaaatataa ttcccacttc catgctggac catgcagatt ctgtcctctg
     1921 tagtgatttg agaatgtgga tcgatgtagc ttgtggctaa acaatcttgc aaagtattgc
     1981 cagtgctgtc agtgataaaa taaaatggca aagtaagttc tcgatctgtt tgtgtcagtg
     2041 gattgcggta aaggatggag aggtccgcaa tataagaatt ctccaatgct ggaagttttc
     2101 tggccacgct aaatttaaca tcattaatga gcaggccgtg ataattttga cccgacgaaa
     2161 accagacttc cggggtagct cccataaaac tggattttag aacacttaaa tttttcgaaa
     2221 ctcccaggga ggagcggctc gtcccaaagg ctgccgcaga aaggtttcgc ttgcaggccg
     2281 ctctattcga aaagaccatt tttagcattg tactaagatg agacgcatct tcgagaaccc
     2341 gatcgcgggc ttgaagcttc atatgaaagt ccagccaaga aataaagacg cccatgagaa
     2401 tcgcaatgga aataactaaa ggcagagcca aaataataat attgccttgt tgttggtttt
     2461 tccccattga cccgccggat ctttgattag caattacaat cttcttagct ttaataatag
     2521 ttcaataatt gattataagt atggaaaatc cccatgtaag cttgaaattt aatgggtcct
     2581 catcctttct gatcggaatt attcttcgaa cacacatcta gaatagatat ctcctatagg
     2641 cgacactgga aataagtcct aaacccgcca tggtggtgag catgctggaa cctccataag
     2701 aaagcagagg taagggaaca cccacaatag gcagcagacc aatgaccatt ccaatattga
     2761 cgaacatatg ccagaacaca tagcagagga cacctattga taacaggact ccgaatttgt
     2821 ctcgggaatt catggcgatt ctaaggccta aaataaataa catacagaaa agtcccatgg
     2881 tcatcatact gcccacaaag ccgtgttctt cactgagcac actgtaaatg aaatcagtgt
     2941 gtcgttctgg taagaattcg agttgtgact gagttccctt gcggaagcct ttccccaaaa
     3001 atctgccgct gcctacggcg attttggatt gaatgctgtt gtaaccagtg ccgcgaggat
     3061 cgttggttgg agacataaac gtgagaatgc gattcttttg atattcacga agaccaaatt
     3121 gccaagccag aggcgcggta atcaccacgg cggtaatgac aacggccaaa atggattttc
     3181 taactttggt gaagagaatc atggaaccgc cgatggcggc caacatcatg ccggttccca
     3241 aatcgggttg ctctacgacc agaacaaagg gcaaaagaag aacgaataaa ggaacggcca
     3301 gttctttgag gcccatccct aagcctgcgc tattgcgtgt agcgaggatt tttgccatca
     3361 gtacgatcaa tgccaacttc attgtctcgg aaggttgata gcggaaaaat cccaaatcaa
     3421 tccaacgttg ggctcctaaa gcgacggtgc cgaagaaggt tgtgtaaagc aaggctccta
     3481 agttcaaaac aaacactccg atggcgagac gagccaccaa ggcataatcc aagagggtga
     3541 aggcaaagaa aacactccaa ccaattccca gccaaacgaa ctgcgagata aaaagaggtt
     3601 ccacatcgcg agagtgaggt ccatgggtcg cgctgtagag attgatcagg ccgatgacgt
     3661 tcaatgcgaa aatcaccaaa aggaaattta aatcgagtcg tttaaaaata gttcttttct
     3721 cgacttgtat tgaaaactcc acgcttactc tccgtccatg attgtggctg gaagattgat
     3781 cttcgcggcc ttcttcagat gaagctcttt aagacccgcc tcgatcctct caggataata
     3841 tttttggaag taagtttgca caatatcccg ggcaataggg gctgcaccgg tgtttccatg
     3901 gcaggcatgc tccgcaagga tggccacagt gatttctgga tggtcggccg gagcccaggc
     3961 aacgaaccag ccatgatggc gcatgtgaat aggtcgtgcc atacaacttg cgtagatttg
     4021 atctgctgag aaattcatga cctgtgcagt tccggttttt cctgccatag gaactccagg
     4081 gactttccag aatctggctg ttccgtgttc accattggca acacgcctca tgccctcttt
     4141 aacaatttta aaggtggcgg gtaaaacttt aacgccggtt ttttgttctt cggtgagatc
     4201 gcgaacgact tgggggaaat tctctttcag gatttttcct tcttggtcga tgattttacg
     4261 aatgataaaa ggctttacca ctttgccttc ggtgccgatg gcattatagg ccacagccat
     4321 ttgaatcgga gtgacttgca cgaagccctg gccaatggcc gtgcttaagt tttcccctgc
     4381 ctgccattct tcacccatgg ccgctttttt ccactccgag ttgggcattg ttccagccac
     4441 ttcacgggcc agttcgactc ctgttttttg tccgattccc atggggttga tataggcaaa
     4501 cattttatca acccccagcg cgattcccat tttaaagaag aaaatattgg agcttctctc
     4561 gatcgcttca aggatcgtga tcatgccgta tccattttta ttgctatcat ggtagggacg
     4621 gcggccaaac atgaagactc cgggggcggc cactaaagtg gtgggtccca ccactttctc
     4681 tgacagagcg gcgacggcga caaaaggttt aaaggtcgag ccgggatagg tataatcttg
     4741 aatgacttta ttgcgcagag gtttaaaagg atcattaatg agttttgacc aagtgggtgc
     4801 ggagaaaccg gtggagaaat cattgggatc aaaagagggg gtgctcaccc aagccaaaac
     4861 ctcgccattt gatttcatca caaaggcggc gccgatccgt ttattgtcta tcatcgattt
     4921 ataagcggcc tcttgcacat cacggtcgat ggtcagtacc gcattgtttc cgtggactgg
     4981 ctcttgatcg cggatttgtt ctccgtaaat attaggcgtg gtggtgacgg tttcgcggcc
     5041 atgggcatcg acttgaataa aactcagacc gtctttgccg cggatgtctt tctcgagagt
     5101 ttcttccaga ccgcttttac cgatgatatc cccttgttca aagaggaaga cgtttttata
     5161 gaggtcattc attaaaggaa tttgacgttt tgaaatctcc gccacgtaac caaaaacctg
     5221 ggcgccgtct tctttaaggg gataatagcg cacgatcgac tcacggattt ccagacctgg
     5281 aatgtccaag cgaatgcgtt ttaaacggaa aacttcttca cggcttaaat tttctttcaa
     5341 acgaacttgc gagaaggggc cattttgctt gcggctcttt tgcacttttt gaacgatttt
     5401 ttcggcttcc atccccaaga tcggagcaac ggtattggcc atggtttcga ggttttcaat
     5461 gtactgcgga gaaagaatag cctcaaagcc cggcaagttt tcaaccaaga ctttgccatt
     5521 tcgatccagc attaagcccc ggggagcgga gattttgttc tgcttgacgc gatttttctc
     5581 cgagaactcc cggagttcat tccctgtgat aatttgtaaa taccacaggc gagcggaaaa
     5641 aatgataaag gtacaggcca ccgcaatata aaacaaacgg tagcggggct ggtattcctt
     5701 ggcctcatcg ggattattta cgtaatcact cacaattcat atcctcgaga ctctggcgtc
     5761 atttcatcca aagtcagctt gtcaattttc atcagaaccc agaacatcgg caagcagaaa
     5821 gccggggtga gtcccaattg gataatgcga tcccaaaact caatgggtgc ggagtttcgc
     5881 tcgatcgcca aagagctaaa gaaataaata agatggtaac ttaaggaagc cacacaacac
     5941 ataattgtat agtaagcagg gccttcccaa aagacccgac tgcggatcga atacagaatc
     6001 agataaacca acagtaaaca gatgaggagc attttcatcg gcatcaccgt gaagcccatc
     6061 aaagccaagc tcataacata gatgataaaa agagccccat aaagtttgcg atagagagcg
     6121 acgtaaacga tcaggcaaag ccaaagcata ggagccggga cagtgccaaa aagttgatac
     6181 cagaaagtgc tttgaaaagc ggctaacacc atcagcacta aaagcagtag gaaaaaattg
     6241 agaaccttat tccaacgaat tttcaccgag ttttctccgc agccggggga gcgggaagaa
     6301 ctcctaaacc tgctccgaca cctaccccca tgccaggagg cagaggagta ggctcagaag
     6361 cctgaggggg aagtcgatcc gagagatctg tattcaaggc attggttaca ataaagactt
     6421 cttccacctt ataaggatcc acgacaggct ttaggtccac ttttaaagag acgctaaagt
     6481 ttttgcgctc tacggattcc acaatggcca cagggaagcc ctttggaaag atattgtcga
     6541 gaccccccgt aaccacgaga tcgccctttt tcacatcttc cgttttttca acatatttca
     6601 attggcaagt tcctggattt ttaccctcaa cgattccatg ggcccgggtt ctttgcacca
     6661 cgccgtccac gacggagtaa cgatccgtga tgagcattac gttagaagtc aaagcttcgg
     6721 gacgatagat ataacccaga acaccttgaa cagtgatgac ggcttgaccg gcttttaaac
     6781 catgtttggt tcctttattg atggtcactg tctgatgatc ggcaaaaaga tccagggcta
     6841 tcacttgcgc actgagcagt tcgagcttcg tagactgacg gaaatctaaa agggcccgca
     6901 gtcggtcgtt ctcgtttttc atttccacaa cttcgctcat gcgagtcaac agttcattat
     6961 ttttgctggt cagctcaaga ttgctttttt tgatattgat cagatctaga tacattgccg
     7021 ttgtgccccg aacgccggcg gaaaatccat aaaaagcctc ctctgccagg cttgcgataa
     7081 ggctgaaagg gcgggagagc cagttagatt cttggggttt ttgctgcata ttaatagaaa
     7141 ccaatggcaa agccaaaacg atgactatca tgatcagctt tttcagatcg atattaaaga
     7201 aattcaacgc tcacctcgtt atatatcgaa acggtaacaa aaagcgtctt tgattccaag
     7261 ggtttccaac agtttctcct tgaaaaaata taaattccgt taggtaatga aaagctcgaa
     7321 acacaggagt tttaaaaata tgtctcattc catggcagat cgtctcagaa agcaagtttc
     7381 cgctaagaac ttcacagcaa tacttctctt cggagctatt attttggtgt ttgtgttttt
     7441 cggagtatca ggacgactcg gtggcggagg cttgggcgcc gccgcacaag tgaatgacgc
     7501 cttgatttca gtagctaatt tgcaggcagc ggaaaacaat ctccgccaga tttacggcga
     7561 agtgtttgga aacgatgcgt ttagactgag agctgaggct ctccgtggct tagtagatcg
     7621 ggaattgatg tcacaagcag ctcgtaaaaa tggaattgtt gtggctgata gtgagattgc
     7681 gcacacaatc actaaagaca ttccggtttt tcaaacgaat ggccaatttc aaaaagacta
     7741 ttatctgcgc tatttagaaa tgacccatca agtccctaaa gaatttgaga acagcattcg
     7801 ccaacaacaa gaagctcttc gtgttcgtca tttgtttgat attgcgtcgg gaatgaccgc
     7861 attggaaact gcaaaaatgg cagatctcaa agcctataaa atgaatgttc aatttgttca
     7921 tatagatcat aatcagttgg cgaagagttt cacggtaagt tctgcagagg ctgagaaagc
     7981 tctcgttaac gcggagtttc ttaaaaaggc gcaggattat ttcactaaga atcaaaccga
     8041 ctatgcccaa gaagaggaaa ttcacgctca gcacatttta ttgcaaataa agccgggagc
     8101 cagcgaagaa gacagtaaaa aagtccttgc taagatccaa gatttgcaaa aaaaagcagc
     8161 cgcaggtgat tttggaaaat tggctgcagc caatagcgaa gatccaggtt ccaaagttaa
     8221 aaatggtgat ttgggattct ttggccgtgg caaaatggtg aaagagtttg aagaagtggc
     8281 tttcagcgct cccatcggca aagtcatggg tccggtcaaa actccttttg gttatcattt
     8341 gattaaagta acagagaaaa aagcggctca gatgcctgac ttcgaaaaac agaaagtcca
     8401 agtagcgcaa aaagttttgg ctgatgagaa aaccgctact ggagtgaaag ctttggaaga
     8461 ggccgtcggc aaaggtgatg agtcggcttt agaattagct cttaagaata taaatgctaa
     8521 atgggaagaa accggaagct tcgatctttc ccaagagtcg gctcctaaaa tcaatagcgt
     8581 tctcgccaac aatgccttgg cggaattgac tttggaacag aaacttttga agcgcattgt
     8641 tcgtgaaggt gacgaaaagt acattttgaa acttaaagaa gcaaaaatgg aggctccaat
     8701 tcctctggat tcaatgacta aaacaatgac ggagtcttat cgtgccaata tgctttttgg
     8761 aaactggctt aacgagttca aaaaagtttc taaagtagaa gtgaacaatc aacttttgaa
     8821 gccgtgaacc tcagccttca ccgctctcat aaaatggccg tgaatattac aaggcccggg
     8881 tttgatgctc gggcttttgt ttttgatgtc gctttgggac tgcgtctgtc ggctgattta
     8941 cccttagagt tgcaagtcga tcctctatcg ggaatgttaa ttaatttggt tcagatggac
     9001 gagctccttc aggaacttga gcagatgtgg ggcgctaaga gctggaattc tttatcagta
     9061 ttattagagg aatcgcggaa atttcttgtg ggacgggtgc ctcaagagcc aggggctcat
     9121 ccgttgacct tgtacgagat tgccctgact gaaaaaagag gcttctggtt gaagtggcaa
     9181 gtgcaaaacg cgggaaggtt tttccaaggt tatgaggaga ttcgggagtt ggatacgaaa
     9241 ctttttcgtt tccgctccga atatttactc catgaagatg gctgggaaca gcagcgccta
     9301 aaatatttaa aagtaattag tacgaatgat ttattttcag agaaaatctt tcaggataat
     9361 ccaggtctac attctctcga gatcgagaac ttgacgactt cgaaaaagtg gtcttttttt
     9421 ctctaaaact tattcggttt ttattagaat ttcaggaata agtcacggcg ccttcgcctg
     9481 ccaattaaca acagtgttca aagacaaggc ccttcgttgg atgtgaaagg ctatcatatc
     9541 ttcattagaa gagtttttga gaggaggatt tatggcaatg cgtgaaccta tgcaagatag
     9601 tgatacggct cattgtatta aaagctgttt gtccaacaaa cgtatttgtc tcgaaaccct
     9661 gcactattgt ctcacgcaaa aaatgacgca ttttcaggga aggcatatcg cccttttagg
     9721 aatgtgcgct gacgcttgcg agttggcggc gaaaatgatg atcgcggaca tccaatttca
     9781 tcgccagagc tgtgtggtgg cctttgaact gtgcctcgct tgcgcggagg agtgtgagaa
     9841 atatatggaa gacacagcta tgaaacgctg tgcggactcc tgtcgaaaat gtgcggaatt
     9901 ttgtcgagga atgatgggga ccaccgttga aatcacttct gcagctgggc aggaccgaag
     9961 gcctccggga ataagtcgat gaatttcaaa gtgcgagagt gtccctgggg attggaaaga
    10021 tgaattttag tctcggggct ggcaaactcc gcgatcactt gacggcagaa accacaggga
    10081 ggccaggggt ttttctcgga gctgaccacc aggacctctt cgattttttt cgctccttca
    10141 ctgatggctt tgaatatggc cgtgcgttcc gcacaaatag ttccaccata ggatgcattt
    10201 tctacattgc agccgccata gattttgcca ttatccatca aaaccgcagc gccaatattt
    10261 tttccagagt agggagcgta agaatttttt tgcccttcaa tggcatattt taaaagttct
    10321 gtaaacatcg tgacctcaag gaatagaaaa gccggagtta tttagtatcc gcgacagggc
    10381 caggaggggc aagccttgaa tagcgctaaa atcctgactt tcaattttat caaagagcat
    10441 catgccgtgc tgctcaattt tataggctcc tgcacaatcc caaggttggt ccaaatccac
    10501 ataacgttct atttgttctt gggaaagcga cttcatgtga aggcgcgtga tatcagcata
    10561 gggggtgcag ctctcaccgt caaaaataca aatagcagtg actagctcat gaatctgacc
    10621 ttgcatacgg agcagttgct caatggcccg ctcacgggtg tgcggttttc ctagaataat
    10681 tccgctgtga gctaccaatt ggtcaccgcc aatcaccacc aagtcagctc ctttgaggct
    10741 ctgagctttc aaaaaagcca acttttccgc taattttagc gggcttaaag aggggtcttt
    10801 ttcggactcc tcatcaataa ggggctttcg agcctcaaaa ggcaggccca attgtcttaa
    10861 aagctcttgg cgatattgag aagtgctggc gaggactatt tttttcatat gtttatttta
    10921 taagatagaa aatcaagaaa ttcgagccaa aatcttggat tttacctcgt ctctgaataa
    10981 acaataaagt cttacatttt ttattattaa agcagtaaga tattttccat gaaatattta
    11041 attttttggg cctgggggat gttttctttt acctgcgctg cttacggagc aactgttggt
    11101 tgtctttcgg gggaatacct ggtgagtggg caccacagaa ggggatacgt tcgttcggat
    11161 ggaacggttg tcagatcaac agatgtaaag tcgtattgta aggtacttac taaagcatat
    11221 gaatactcaa agaaacgttt taaaaagggg cgcccagcta attggcccca caatatagaa
    11281 aaagccagtt catggactga tgaagagact gaaaggattg ttgaagtatt agaagagttg
    11341 ccggaggttc ttttgagtaa agtcattgtt ggaatttatc gactgaagag atctaaagac
    11401 tatcccaatc ctgcaagtag ttcggacggg gtcattgtga tctatgattc aagcttcgat
    11461 ttgtcgcgga atttaggcag gataatagct catgagctat cccatcaaaa ttttaaagat
    11521 ctaagtaagg acgaaaagca ggactatcgt cgtgctacag gctggcggtt aaaaatagaa
    11581 tcagacagaa atgtttattg ggtgggacgt aaggatggct atgtggaaga agatggaaaa
    11641 atttcatatg aagaagacta ttcaaataac cttgagcatt atctttatga ccctgataaa
    11701 cttaaaaggg taacgccaag tgcttatgag tggattaaaa aacattttgg tgatggcttt
    11761 cagattaaga ggcaaaagag atgaaattta aaggaatttt aatgacatta tttttatctg
    11821 cgtgtgtgac caataccacg ggttctaacc gaggctcttt gaattacgat aaggtacgta
    11881 gtttggtggt tggatattcc acagaaagtg atgtcctggc ggttttaggc ttgcctacag
    11941 gtcgtacaga agaggcaggt tattatatac taaactatga tgattcgaag actggtttcc
    12001 agcgtttatc cataaatttc ttattaggaa gtcaaaagct ttcgggcatt ttgtggattc
    12061 cgcgagaaga cgaaaaggaa tattctttaa tgcaagcgaa agctgggttt aaaaatgctg
    12121 actttaaaga aattatcaat aggagcacca atccgcatgc gatttctcca gatgaggttt
    12181 cgtacatcga tgaaaagcat ggagttacaa ttcgttatga tcgaagtcaa aatattgttg
    12241 aggccatagc gatgtatgat gtaaatgttc gattgcccgc ggcaacagag aaaaagaatc
    12301 agattcccta cactttcggg gatgagcctg ctatttcaaa atagaaatct gcgaattatt
    12361 caaacgagtt gaaaatggac aaacagaaac ttcctgacat cgccatggaa accagaacgg
    12421 aaaccagaaa agacctttat ccccccattg atcaagtggg gatgggaaat attgaagttc
    12481 cagttttact ttcctccgat gagacgatgc tatttcgagt tccagcccgg gtggacgcaa
    12541 aagtgagttt ggatcgtgct tcgtcacgtg ggattcacat gtcgcgttta tatctctctg
    12601 tacaagaaat gctctccagc aaagctttaa gttattctct tttggaagaa ctctctcaag
    12661 agtttttgaa aacccatgag gatctcagtc gtcgctcttt tgtcacagtt aagtttgagg
    12721 cccctgtgca acgaaaggct cttaaaaccc agaataaggc ttggcgcacg tatcccgtgg
    12781 ctttgtcggt ggaaaacttt gaaggccgcg caaaaatttt cgctgaaatt ttagtgactt
    12841 attccagtac gtgcccagcc tccgccgctt tgtctcggca gcttattcaa gaaaatttca
    12901 agcaagaatt cttaagccaa gatgaattaa gttttgcgga tattcatcaa tggcttggca
    12961 gtacccaagg cgtgattgca actccacatg cacaaagaag caaagccaga gtgaaggttc
    13021 agttccactc tgcaagttcc tctctgagtt tctcagaact cattgatgtg gttgaggacg
    13081 cagtacagac ccccgtccaa ggggctgtca agcgtcagga tgaacaagaa tttgcccttc
    13141 gcaatggaca gaatctaatg ttctgtgaag atgcctcccg aagaattaaa acaagtttag
    13201 atctgcgccc tgagatcagc gattatttga tcaaggttaa gcatatggaa agtttacatc
    13261 ctcataatgc ggaagccctt atcgccaaag gtgtctctgg cggattgagc tagccccacc
    13321 gaaatcgccc ccactgacaa tatacattgt atagatcgtg aatccttcca ctgaagggac
    13381 aagcttcccc tatagaagtt gttattctta agagcattaa aaaatgttgt tttgtgagga
    13441 ggttttatgt ttacaagaaa aatagtcacg gcggcggtga tggcaatggc agtgaacgga
    13501 acagtctttg ctgtttccca ttctgacgct gacaatacga agctcaatca gcgtgatgct
    13561 aaaaacacta agcaaagtgc tttaactcca gacattcaaa ccaaggggtc tcctcaggac
    13621 gtggaattga ctcgccttct tcgtcaagaa ctgaccaatg ataaaactct ttcgacgaat
    13681 gcgcaaaata tcaagatcat cacgttggat ggaatagtga ctttaagggg gccggtagag
    13741 agccaggaag aaaagcagcg catcgatgcc ttagcgaaaa aaactgctgg agtcaaaaga
    13801 gtcgacaacc agttagatgt gaaatcaaaa gcttattagt tagaggcgat cgtaattatt
    13861 taaggagga
//
LOCUS       DATBTE010000028         3868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1438090, whole genome shotgun sequence.
ACCESSION   DATBTE010000028 DATBTE010000000
VERSION     DATBTE010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438090"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1684
                     /locus_tag="VIG33_00975"
     CDS             <1..1684
                     /locus_tag="VIG33_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269432.1"
                     /translation="GYYVLNTHVPNITKWLQKIQIGVDPKLPTDAVFQNNILQEIADF
                     NKGVKTLIAQYNSDLATLKGMKDLETQKNTVLHLIVTLVESMGGSQGGFTQQGAGRKN
                     FFTMSKNALRIPFDLVGMSAPDQVLGRLMPIMDYDQWFRANMDTLPVFNDPLALAETI
                     GHNLQEVIRAASVAAIEYFNRWYIVDKAALINESTIDINYTVQESLIAVNRYLESEKQ
                     RIDKYNGTASLVPTIVDTQIRINNILKGYAELEELGKKTLSDKDSLNQTAEQVQAVAD
                     KYEKLVNTVYEQFNVMQSRSGFIANRMVNFVYEDYILLLKNKVDFTQYQDDLATATGM
                     AAMDKMMQIYNGNPANIQTDLNMALRIDKGNIEALQALLKDSLIRAINEQDIVKNGGK
                     NTYWNTMTRLVKDTVADTVREKSWFNKTIMGGSFLQFAWMTPKTASYYLKHSDRYLFS
                     ANGLMNESPQSEFQDAENVKAQLCIQALAFYDQSTLNNLCHGVVLKSPFGEAVTSMNM
                     SYDEKLWEHLRNSKLSVQMRQSLNHSDRICAFRDYNMRNMARFMSVGKNSVK"
     gene            1695..2810
                     /locus_tag="VIG33_00980"
     CDS             1695..2810
                     /locus_tag="VIG33_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269433.1"
                     /translation="MKNLKISPWRFIFLLGALHLCCASLSFAVGADTSWKMGLSTVSA
                     QDTQSASKFVGFALDAKMKYWLHPELFANLDPNITFENGSFQSVDGERKNQSGLYLKE
                     AGVHWLFMRGSTLSGGALNQATVHTDLLTGDQAFPALRGELELFNVGNLTTSLDLEQA
                     IPTSNSLSTNTTSVESTPRFLSASLTLNYESTPYFWKTRVGAFSYDNLPSAVAYQSGL
                     RGNTIVSLTESESSFVYQYQGLEAMTSLRFPLMRGWDFTTSASYLQNDKAVSTLNRAY
                     SLSAGSEFFFVGRKSVDINLTSFRIEPDAAVAYFNSGKYFNTNRIGYNVESFIKFNKY
                     NFKIGLGYTEAEVIYLHPIQSREKLFMLMLETFYANI"
     gene            2797..>3868
                     /locus_tag="VIG33_00985"
     CDS             2797..>3868
                     /locus_tag="VIG33_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269434.1"
                     /translation="MPTSKLFLFSILSLNLLGITACNIPSPQLRDMTRDSEGQGIATF
                     FITDVKGEALAAKLNKDSATPQSKTFNFTACVKDQAQSKPLIGHPFRVEEISKGLKTD
                     EKGCLNWSEEVGFNFLSAPRYLEWKRKITASGLHRGTRVARFAINPWSDNDNSQAVVN
                     LDTFTPPLLTSEPEKIQEALKGSSENSSLTKNSLWLNNISLQSTEKKFTSNGVILNFE
                     LVASPQLQVSKINGETMLRSLSQGRFKLKFYLIHILLEDNKEIHRLLSQSDPQTVEMN
                     GGSLFISFPLSAPRLPSVGQLALGIDISGEGKDTEIGNFQGIYTLGDYDQIKGSRLLR
                     LMSIVTENKEFKISSFINSNMDE"
BASE COUNT         1127 a          837 c          842 g         1062 t
ORIGIN      
        1 tggatattat gtcttgaata cgcatgtccc caatattacg aaatggcttc aaaaaatcca
       61 gatcggggtg gatccaaagc ttccgacgga cgcggttttc caaaacaata ttctgcaaga
      121 aatagcggat ttcaacaaag gagtgaaaac tctcattgct cagtacaatt ctgacctcgc
      181 cacgctcaaa gggatgaaag atcttgagac acaaaaaaat accgttttgc atcttattgt
      241 gacgttagtg gaatccatgg gaggttccca gggtggcttt actcaacaag gagcaggaag
      301 aaaaaacttt ttcactatga gtaaaaatgc gcttcgtatc ccttttgatc tggtcggcat
      361 gagcgctccc gatcaggtgt tgggtcgctt aatgccaatt atggactatg atcagtggtt
      421 ccgggcaaat atggacactc ttcctgtgtt caatgaccct ctcgccttgg cggaaaccat
      481 tggacataat ttacaagaag ttattcgtgc cgctagcgtt gcagccattg agtatttcaa
      541 tagatggtac atcgtggata aggccgcttt gatcaacgaa tccacgattg atatcaacta
      601 cactgttcaa gaaagtttga ttgcagtgaa caggtattta gagtccgaga aacaacggat
      661 tgataaatac aatggaaccg cctctcttgt tccgaccatt gtagacacac aaattcgcat
      721 caacaatatt ttaaaaggct atgcggaatt agaagaactc ggaaagaaaa ccctcagtga
      781 taaagacagc cttaatcaga ctgcggaaca agttcaggca gtggccgaca agtatgaaaa
      841 actcgtaaat acagtgtacg aacagttcaa tgtcatgcaa tcgcgatccg gttttattgc
      901 caaccgtatg gtcaattttg tttatgagga ttatattctc ctgttaaaaa acaaagtgga
      961 tttcacccaa taccaggatg atttagcgac cgcaacagga atggcggcga tggataagat
     1021 gatgcaaatc tacaatggca atcctgcgaa tattcaaacg gatttgaata tggcattgag
     1081 aatcgataag ggaaatattg aggcattgca ggctctgctt aaagacagtc tcattcgcgc
     1141 catcaatgaa caggatattg taaaaaatgg cggcaagaac acttactgga atacgatgac
     1201 tcgattggtg aaagacacag tggctgatac tgtcagggaa aaatcgtggt ttaataaaac
     1261 gattatgggc ggaagttttt tgcaatttgc gtggatgacg ccaaagacgg cttcctatta
     1321 tctaaagcac tccgatcgat acctgttttc agcgaatggt ttgatgaacg aaagtccgca
     1381 aagcgagttc caggatgccg aaaatgtgaa ggcccagttg tgtatccaag cccttgcttt
     1441 ttatgatcag tcgaccctca ataatctttg ccatggggtc gtcttaaaga gcccctttgg
     1501 tgaggccgtg acttccatga atatgtctta tgacgaaaag ctatgggagc atttaaggaa
     1561 ttccaaactt tcggtgcaga tgcggcaatc attaaatcac tcagacagaa tctgcgcttt
     1621 ccgcgattac aatatgcgaa atatggcccg cttcatgtcg gtaggtaaga attctgtgaa
     1681 ataaagaagg tgctatgaaa aacctcaaaa tctcaccatg gcgttttatt tttctattag
     1741 gggcgcttca cctttgctgt gcctctttgt cttttgccgt gggagcagat accagctgga
     1801 aaatggggct ttccactgtg agtgcccaag atactcaatc cgccagcaaa ttcgtaggtt
     1861 ttgctctgga tgcgaaaatg aagtactggc ttcacccgga actttttgcg aatctggatc
     1921 ctaatatcac ctttgaaaac ggcagttttc aatccgttga tggcgaacgt aaaaaccaaa
     1981 gcggcctcta tcttaaggag gcaggtgttc attggctctt tatgaggggg tctactcttt
     2041 ctggcggagc tttaaaccag gcgaccgttc acaccgatct tttaaccggt gaccaggcct
     2101 ttcccgcgct gcgcggagag cttgaacttt ttaatgtggg aaatttgacc acttccctag
     2161 atctcgaaca agcgattccg acttcgaact ctctcagtac aaacacgacc tctgtcgaat
     2221 ccacaccgcg atttttaagt gcctcattga ctttgaatta tgaaagcacg ccctactttt
     2281 ggaaaactcg cgtgggagct ttcagttatg acaatctccc cagtgctgtg gcttatcaaa
     2341 gcggacttcg cggcaatacc attgtatccc ttacagaaag cgagtcctcg tttgtttatc
     2401 aataccaggg actcgaggcc atgacctctt tacgatttcc cctgatgcgt ggctgggact
     2461 tcacgacttc cgcctcttat ttacaaaacg ataaagccgt tagcactctc aaccgcgctt
     2521 attccctgag tgccggatct gaattctttt tcgtgggcag aaaaagtgtc gatataaatc
     2581 tcaccagttt ccgtattgag ccagatgccg ctgttgctta ctttaacagt ggtaaatact
     2641 ttaataccaa tcgcattggc tacaatgtag aatcttttat taagtttaat aaatataatt
     2701 tcaaaattgg cctgggctat accgaagcag aagttattta tctacatccc attcaaagtc
     2761 gtgaaaaact ttttatgctt atgctggaga ctttctatgc caacatctaa gcttttcttg
     2821 ttttcaatcc tcagcttaaa tttgttggga ataacggctt gtaatattcc ctctccccaa
     2881 ctccgagaca tgaccagaga ttccgaaggt cagggcatag ccactttttt tatcactgac
     2941 gtcaagggcg aagcgctagc cgccaaacta aataaagatt ccgccacgcc tcaaagcaag
     3001 actttcaact tcacggcctg cgtgaaagat caagctcaat caaaaccctt gatcgggcat
     3061 ccgttccgag ttgaagaaat cagtaaaggg cttaaaacag atgagaaagg ttgccttaac
     3121 tggagtgaag aggtgggctt caactttctt tcggcgccaa gatacctgga gtggaagcgg
     3181 aaaatcaccg ctagtggact tcacagagga actcgtgtcg cgcgctttgc gatcaatcct
     3241 tggagcgata atgacaattc gcaagccgtc gtgaatctgg ataccttcac ccctccacta
     3301 ttgaccagtg agcctgaaaa aattcaggaa gctctgaagg gttcttcaga gaacagttct
     3361 ctgactaaaa atagcttgtg gctaaataat ataagcctac aaagcaccga aaaaaaattc
     3421 acttccaatg gagtgatatt aaattttgaa ttggtggcct caccacaatt gcaagtttcg
     3481 aaaatcaatg gcgaaacgat gttgcgctcc ctctctcagg gacgttttaa gttgaaattt
     3541 tatcttattc acattctcct ggaagataat aaagagattc atcgcctttt gtctcaatcc
     3601 gacccgcaaa ctgtggaaat gaacggtggt agtttattta tcagctttcc tttgagtgca
     3661 cctcgtcttc cctcagtcgg ccaattagca ttgggtattg atatttccgg ggaaggaaaa
     3721 gatacagaga taggtaactt tcaagggata tatactttag gcgactatga ccagataaaa
     3781 ggttcacgtc tcttgagact catgagcata gtcactgaaa ataaagagtt caagatatca
     3841 agttttataa attcaaacat ggatgaaa
//
LOCUS       DATBTE010000029         3221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2257952, whole genome shotgun sequence.
ACCESSION   DATBTE010000029 DATBTE010000000
VERSION     DATBTE010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2257952"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VIG33_00990"
     CDS             <1..193
                     /locus_tag="VIG33_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269435.1"
                     /translation="GISELRINFGPGSRVYYMIVDGNTVILLLTAGDKSTQKDNMAQA
                     KKYYADYKKRSGEAKDEKK"
     gene            278..718
                     /locus_tag="VIG33_00995"
                     /pseudo
     CDS             278..718
                     /locus_tag="VIG33_00995"
                     /inference="COORDINATES: protein motif:HMM:NF044550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
     gene            884..1114
                     /locus_tag="VIG33_01000"
     CDS             884..1114
                     /locus_tag="VIG33_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269436.1"
                     /translation="MIAKKLTKVGNTQAVVLDKTLLSLVDADTESTFKISIEGKKIVL
                     EAMSQKEVDRLALEASDKIRKVQKNVFKKLVK"
     gene            1295..2278
                     /locus_tag="VIG33_01005"
     CDS             1295..2278
                     /locus_tag="VIG33_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY8269437.1"
                     /translation="MKSWLTIGQFSKQVSLSQRTIRVYEKAGLIKAHTRGENEYRYYK
                     IEQAEIIDRIKQFKAFGFSLGEIRSLLEIDASMDHEKLCALLQRQLVALQQEHSNIEL
                     AQSMLKQVLISLNQNKPGLAPEERRFIMNRFEKTSVLVGGVSNLELTANLIRRHFEKS
                     GKSVPVIVWNGYDDFPATKPYIVVVSEDLLKNPKVSSLTPDVVVIKELSRSSDDLRRA
                     YLQVYSAVGPNMSTILNADDRAVVEFAENEILRKGRTYYFSKNSGLQAQISRIGGVVS
                     DGEKVSIYGLNQKGVPTEINLDRILGYNEEMAYLASLAAIMDFGLKREALV"
     gene            2407..2709
                     /locus_tag="VIG33_01010"
     CDS             2407..2709
                     /locus_tag="VIG33_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269438.1"
                     /translation="MHRRVFSLSTQSTKSSHREAEFYFVVEVKDGILQVEFEEVAEPK
                     GLLASVSGTFAQGYQDEQSYVLSDSQWDRGQHISLRASANSLGVWGDLLTKESPPF"
     gene            complement(2687..>3221)
                     /locus_tag="VIG33_01015"
     CDS             complement(2687..>3221)
                     /locus_tag="VIG33_01015"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY8269439.1"
                     /translation="VASVNMDLLGGGMHAQLARTLRTERGLTYAAMSRFSKSTLPFWM
                     VWTFGGVEQTKGLLTGVPEVVGKYVAAPLTAKDLTESQGRLLNSNGTELELAQDRLLT
                     QSWFYANGLNPEAVEQYSKSVGKTKLAEVTKFKNILSSKAAAIYVMGDQTKLIPILES
                     IGVPRAEIRVVKMAEIL"
BASE COUNT          961 a          670 c          737 g          853 t
ORIGIN      
        1 tggaatcagt gaactgagaa ttaattttgg accgggttct cgagtttatt atatgattgt
       61 ggatgggaac acagtgattt tgctgctaac ggccggtgat aaaagtactc aaaaagataa
      121 tatggcccag gcaaaaaagt actatgcaga ttataagaaa cgatctggag aggcaaaaga
      181 tgaaaaaaaa taaagtgact aagaaaactt ctaaaaaagc aatttcatca atcggctctg
      241 attttatgaa catcttgagc tagaattaac caatcctcaa agtgctgctg agtttataaa
      301 tgcggcagtt gagatgaatg atccagaata tttgaaagta gcgcttggac gcgtagctaa
      361 agcgcgagga attaaggaaa tttcaaattc tacaaatttg ggaagagaat ccatttacaa
      421 aatgtttgct caagatagta atcctgcgct tgcaagtatt cagtcgattc tagacgtttg
      481 cggtcttgag cttgttgtgc gttctaagaa tgaacgaatg gaaattccaa tggcatctga
      541 gttgagaaaa attgtgaaga aggaagtcaa agactctgtt aaagaagcac ttgaaacgag
      601 agttgcaatg gcggctatta aatattcaaa gaagtctaag ccatcaacat cgtattccag
      661 gccccttaag gcgactgcca ctcttaagaa gagtaccaaa aaagctactt cgtactaatt
      721 attaaaatgg taaattacat tagagattga ggggttttag tccactcttc gccaaacaaa
      781 tcctggagaa ccactactta ttctccacca cactatccgt tctccaattt aatatttatt
      841 tttagtatac caatgtatac ttaagatcga cctggaggtt tatgtgatag cgaaaaagct
      901 aaccaaagtg ggcaacactc aggctgttgt cttggataaa acgcttttga gtcttgtcga
      961 cgccgacact gagagcactt tcaaaatttc aattgaaggt aagaagatcg ttcttgaggc
     1021 catgagccaa aaggaagtcg atcgccttgc attagaagcc agtgataaaa ttcgcaaggt
     1081 tcagaagaat gtcttcaaga aactcgtcaa gtgagccttt ctacatttca ttgaacgtcg
     1141 ttctcgagat tcatcagcgt cagctgaaag aattggtggc gctgatggaa tacgaagccg
     1201 atcaggtctt gaagctgccg tcgaaagtcc agtgctcaaa aaaaatgctt gagcttgacc
     1261 caacgtcaac ctttacgctt cctgagaggt taagatgaaa agctggctca cgatcggaca
     1321 attttcgaaa caggtctcgc tttctcagcg aacgattcgc gtttatgaaa aggccggtct
     1381 tatcaaagca catacacgag gcgaaaacga atatcgctac tacaagatcg agcaggcgga
     1441 gatcattgat aggattaagc agttcaaagc cttcggtttc tctttgggag aaattcggtc
     1501 actgcttgag attgatgcgt cgatggatca tgagaagctt tgtgcgttgt tgcaacgaca
     1561 actcgtcgct cttcagcagg agcattcgaa catcgagctc gcgcagtcga tgttgaaaca
     1621 agtcttaatc tctctcaatc agaacaaacc gggcctggcg cccgaggaga ggagatttat
     1681 catgaatcgt ttcgagaaga cttcagtctt agttggtgga gtaagtaact tagaactaac
     1741 ggcaaacctt atcaggcgac attttgagaa aagcggaaag agtgttcccg tcatcgtttg
     1801 gaacgggtac gatgactttc cggcgaccaa gccatacatc gtcgtcgtgt ccgaagatct
     1861 actaaaaaat ccaaaagttt cctctcttac tccggacgtc gttgtcatca aggagttgag
     1921 cagatcttcg gatgacttgc gcagagcata tctgcaagtt tatagcgccg ttggaccgaa
     1981 tatgtcgact atactgaatg cagacgatcg tgccgtggta gaattcgccg aaaatgaaat
     2041 tcttcgcaaa ggaagaacat actacttttc taaaaattcg ggattgcagg cgcaaattag
     2101 cagaattggc ggcgtcgtta gcgatggaga aaaagtctcc atctacgggt tgaatcaaaa
     2161 aggggtcccg accgaaataa atcttgatcg catcttagga tacaatgagg aaatggctta
     2221 tttggcctct ttggctgcga ttatggattt cggattaaaa cgagaagccc ttgtttagtc
     2281 gcacgtgaaa aacaataggc ctttggaacc agcacgtgac cgagaagaga tgccggtttt
     2341 cgacgaatcg attttctacc ccgaaatcga aactcgaaaa cttggtttca ctgacgaaga
     2401 catcgaatgc atcgccgagt attttcgctg tcaactcaat caacgaaatc gtcgcatcga
     2461 gaagcagaat tttattttgt tgttgaagtt aaggacggaa ttttgcaggt tgagtttgaa
     2521 gaagtcgccg aacccaaagg gcttttggct tcggtgtctg gtacatttgc tcaagggtat
     2581 caagacgaac aatcttatgt tttgtcagat agtcaatggg accgaggcca gcatatttcc
     2641 ctgcgggcct cggctaattc tctaggggtt tggggtgacc tattgactaa agaatctccg
     2701 ccattttaac aacgcggatt tcagcgcgag gaacaccgat ggattccaga atcggaatca
     2761 atttcgtttg gtctcccatc acgtaaatag ctgcggcctt cgaactcagg atgttcttaa
     2821 acttcgtcac ttcagccaat ttggtttttc caacggactt tgaatactgc tccactgctt
     2881 ccggatttaa gccattcgca tagaaccagc tttgtgtcag caaacgatcc tgtgccagct
     2941 ctaattcagt tccattcgaa tttaaaagac gtccttggga ttcagtaagg tcttttgctg
     3001 ttaaaggagc ggccacgtat ttgcccacca cttcaggaac tcccgtcaga agccccttcg
     3061 tttgttcaac gcctccaaag gtccatacca tccaaaatgg caaagtgctt ttactaaaac
     3121 gagacatggc cgcataagtt agtccgcgtt cagtacgtaa tgtgcgagcc agctgagcgt
     3181 gcatacctcc acccagtaaa tccatattca ccgaggcaac g
//
LOCUS       DATBTE010000030        15146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_72084, whole genome shotgun sequence.
ACCESSION   DATBTE010000030 DATBTE010000000
VERSION     DATBTE010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 15146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15146
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72084"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VIG33_01020"
     CDS             <1..882
                     /locus_tag="VIG33_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269440.1"
                     /translation="SLTKWNLMRALSRMLLIGYGSTHNPEVAKEVMKEPGLEQWYADF
                     NQFGIDLKAFDRRSQNSGVRSFKEANFFTYKGNGDSLMDYNETFDFVSLLVSGGKSSA
                     DFIREDILKSGCGTSDKDIFDYYWMDEVCFKRTLRKKFAVYFNNLPGMASEVSEMSEL
                     QWNGFYGNLMGAARVSAATGGRVETADLRTAVMILHYVESLMSVYDGNNDGKLNVEEL
                     RIASSRFLNFMKTVSPIDANFMVEDFFLFLVHKGHKPTLGEYTYFQGEKAFDSLKDVG
                     RDKILRVFKVLKDEAAH"
     gene            940..1956
                     /locus_tag="VIG33_01025"
     CDS             940..1956
                     /locus_tag="VIG33_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269441.1"
                     /translation="MNKNWFLFLLLFSCAAFAGKSTSFEKGWARGYVQSIDSARTMTV
                     LTAYSEFSLGEILVLQSRDPSQDIIGFVQIESIETQGDGQYILKARLVRHSKFAMVQL
                     GDVLYKMEFNTFHREYHGTTELLMRNEDPESSSSYKSLIFMGLGAGETAQTLRENEFL
                     VDIFGYLDYGVTANLTLGTLLPADLFSAPNLHAKYRVYQSDSSVTSLGLNYFQSPDTS
                     SGIINMSLYWDSVSSESVIAHTNLSLALATFDKAKNLTALKGAGTSSLQSGYEFIMDN
                     WNRVLVGPSYNFENKSIGGYLSYVWIWDTFHLLAGVSTADISNVIYNVEKGYYVNIDM
                     YWRF"
     gene            1984..3186
                     /locus_tag="VIG33_01030"
     CDS             1984..3186
                     /locus_tag="VIG33_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY8269442.1"
                     /translation="MKESFYISCNLGFEETLAQEIRQCWPQLIEINGQANQSPLPELT
                     LEMGGVLVKTSFELGLQFNFFLKTAHRVLWRIAEFKVRDFPKLFEKVQKITWGNYLKG
                     RKIEWVVAASKSRLNNEKRIEETCGEAFAKVAAKVQGESLAPQAVYVRIYEDLCTLSL
                     DSSGENLYKRGWGTLKGEAPLRETLAAFLIQEMMQDAPPNKTQQVTVVDPMCGSGTLI
                     LEAAGLWQPVFERKFSFLDWKNTPKIFRSSLLKKNYKLLQKQAPFKSYLGYDIDEKVL
                     AAAEENQKSLESKMDVKNLKIRFEKENLFKGEKKNLGPVWCITNPPYGERLHVQGRDS
                     RDQEAFSYQDLLQRMADKFSAEKIGVLLPNKRSVKSLKAPEGYKKRKEIPFSNGGLEV
                     VYMIYRKK"
     gene            complement(3200..3817)
                     /locus_tag="VIG33_01035"
     CDS             complement(3200..3817)
                     /locus_tag="VIG33_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY8269443.1"
                     /translation="MIQIYGSPRSSAGRCFLMLEECGLPYEVRPLDMGKKEHKSASFL
                     KLNPNGKVPCLIDNDFVLWESAAIVHYLADKYKPAMLGLNTEDKALVNQWTFWSMTEA
                     QPPLVDILIQKVFVPEDKRDLALIARREQQVPALFEVLEKELINKKYLVADTYSVADV
                     MAGSVMNMAMGLQVDLNPYPNIKSWLSVMRSRPAWVKFAELRQAK"
     gene            complement(3856..4947)
                     /locus_tag="VIG33_01040"
     CDS             complement(3856..4947)
                     /locus_tag="VIG33_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8269444.1"
                     /translation="MIVFNVAFNNCGEGFVLQQAPSGNSVLFSRAAGQTCEDALLKVY
                     QGTYHPFLSQTCNNCHVNGPGIGTFASSDVQLSFNSFYAIGVNKISSQATNNSHKPPY
                     TGSQNTDRMNELQSYWSTAQVQYASCVSESGGGSLQSVVKTIDKTVAATLSTTFVRIE
                     WDLETESNVKIPLIAGIDIRKAVLNKLTEGYEFRNPTLRLKRANAGTYQSRALNLYIN
                     GQLQTEVTTYSNIDLSVSSTMDVNLAPGYANGYAVLTPANSDVIALEFSSLKSASNAP
                     PPSMGTTVKYSQLTAAGGIFANSCVGCHNATNLGGALDLTNYIAAKTARAIIRSRINN
                     AMNPMPIGGLLPQAQRDIINAWVDQGAPQ"
     gene            complement(5043..6467)
                     /locus_tag="VIG33_01045"
     CDS             complement(5043..6467)
                     /locus_tag="VIG33_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269445.1"
                     /translation="MGKNKKKIPLWAQTGHAKPVTRREFLSAGILPFAASLAIPNWVS
                     LLLGSQAQAETGLDCPLPASLIPFVTLNLSGGAALGSNFVPMNAKREPIASYSKMGLG
                     DNKVPLEMEFGNVPFAGMNNGVLISKFLVGVRNQATAPTLANTAFIGIPCDSQNDSPN
                     NKFDISGAIAKAGLIGTKFPNLGRVNNRTGINQASAIIAPPAPLVVNDYGALLTSIGY
                     SSALGKALNPEQKNSLAKLMSNLNTIQTRKLASIQGASGVKKVLDCAGIKNIDVVAQG
                     ASAIDPRQNTAFSQVWGVNATTANNDRELIFGSMIYNTLSGQAGASSLEMGGFDYHNN
                     TRTTGDAKDQEAGTTVGRILQSAAVLNKPVFIYVISDGSVSCQDSPNRNAPWTSDRGS
                     NGIAYMLYYDPKGRPATSDYQIGQFNDNQSADTSFITGGNPEMVAAAVFANWCQANKR
                     NDLFEKIAGRVFDTEQLSQIIKLA"
     gene            complement(6489..7148)
                     /locus_tag="VIG33_01050"
     CDS             complement(6489..7148)
                     /locus_tag="VIG33_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269446.1"
                     /translation="MLNKPSKVAILCVPLIGVIIFGFNNCGRFSQPHEESTENASLGS
                     ESLDSQQLGLPYALLSAEQTLSSMMKVTNVTTLSPALKAEYKGRYGALAAGNDLSMAN
                     GPLMLGSTSLAGEICNSLLIQEKAMTDVTSRSFFGSIDFTTGVSGLEDNGLNTSIRGM
                     ARSFWGRNETLEELTLLQEFRTDFINALTETDKTKPVAIRNLVLATCAAMLSSVDAIS
                     Y"
     gene            complement(7159..8421)
                     /locus_tag="VIG33_01055"
     CDS             complement(7159..8421)
                     /locus_tag="VIG33_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269447.1"
                     /translation="MSLAYSLLPLVIVSAFAPFCHAQVKPNIMAAEPSAVEQKARKMY
                     ERLTGAKLPIDSPVINQMVDLLNKGDLPGAAQIATADPNFYNITVKLMALALSTREET
                     IKTPLNDMAADFIGVTRDGKDARELLTGNFYYMGSGAGIRSELINDLLLSNNHYADLE
                     SNRVDLAANLVRVEGQMIATSASATAPNPDPAGVLTSRAFMGAHALMGTNRRLLEYTF
                     REFMCVPKEGWADAEASDARIGRDVDRFPAGDHMKYLTSCKGCHTVMDGFRGAFAKWD
                     FDGKGLKHSAVNARGGGDFGIAADANGVANKMNKNNTIFPSGYIMTDDSFVNNAIRPS
                     NASLFQWRGNSAIGKGVKDFGAIVANSRRFSQCMAKRAFETVCRKMVDATKDRSKLEK
                     WGDEFEASGYQLKKLFESVAGKPECLGN"
     gene            complement(8600..9022)
                     /locus_tag="VIG33_01060"
     CDS             complement(8600..9022)
                     /locus_tag="VIG33_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269448.1"
                     /translation="MLKALMNTLTLVLSLSFSFSAHAFGDENTETKIQAQVLMNAGQM
                     QTEASILLKEISDIVTEVGTSMEDSGGFLKALGVNETAATIAKKKIDKLLAIMNRSQS
                     SNKIFKGYSEVETLTREIRALNQDSKSILESLRNTKSI"
     gene            complement(9136..10326)
                     /locus_tag="VIG33_01065"
     CDS             complement(9136..10326)
                     /locus_tag="VIG33_01065"
                     /inference="COORDINATES: protein motif:HMM:NF040322.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY8269449.1"
                     /translation="MKYSPICFFTLFAASFFIFTLGCTTSGPKTTMETKEIREKTPRP
                     DEVSVMNFNVENLFDTIHDENRDDYAYLPLLQKQTPEHKAVCAKINQIHYRKECLTLD
                     WNEDVVNAKLKNLAQVILGVDGQGPDILVMEEVENERILTRLNKEFLTKAGYQTQVLI
                     EGSDERGIDVALLSRFPIVGTPELHKIPYVAKNDSDKIGMGKSRGILEVKLKLPNQET
                     LTLLGAHFPSQANPRYWREQAVEFLAKMIQESANNMVIASGDLNISHNEEIEARFFHD
                     SFSEVALVSHLVGCKKCEGSHNYRKEWSFLDALLFSKNMGESGTASYKLEPETITIVH
                     SDPIHLWGSYPKRFKPETKEGVSDHFPLYARIRLRAKMAVPVADVPTKFSSCRFFGQK
                     QSAP"
     gene            10469..11347
                     /locus_tag="VIG33_01070"
     CDS             10469..11347
                     /locus_tag="VIG33_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269450.1"
                     /translation="MRFSRFWMVVSTALFTSFIASAQTAGTNILEVSPGSEAPSKWGV
                     SFYSLGSIAQKQIETGDPSYFTYNYIGLNYKISKSRRFAIRPVFNYVSSGKDKYANDV
                     KSEMTTGDLHIVYADYEIATLGEANVSTSFKFYLPTSQGSQDSQMIVKFRPETFISME
                     VRRHDILTYALKPDFFLQSRGSYLDANRKDRSTQIALIEHYLEYSAGLNKMFSLKPSV
                     GFIESWFNPSTNKNFNTHKTEAKIALGLDINLMKGLMFTLSAENRVLLTDRKDAVTFF
                     RPEDNGLVLITSASLL"
     gene            complement(11325..12257)
                     /locus_tag="VIG33_01075"
     CDS             complement(11325..12257)
                     /locus_tag="VIG33_01075"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY8269451.1"
                     /translation="MAFIAPLEHNRHAVLSQQKVMQSNNRETNHYNNGFEYGVKHILS
                     PKSGLLNEILISEFSFSAESFDELLKLGSLYLEGQRLHENCLIVEGAYIRIHSKPRRF
                     PTNDYNWSERILFQDENFVVVNKPAGLPMHASVDNCLENLHKYLSAYLQTELFVTHRL
                     DVPTSGLVVLAKNREFLPLFNHLLAQRGVRKIYRARAEGLKLKDGLYTHYMEPSPRAP
                     KKVSEEPQPHWQECKLRLFDIQDHVLLNEQEVHVELLTGRTHQIRAQLSALGAPICGD
                     ILYGAQKKYDFEKIDLEAHSLQFLDFNFTVTKHW"
     gene            12282..12956
                     /locus_tag="VIG33_01080"
     CDS             12282..12956
                     /locus_tag="VIG33_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEY8269452.1"
                     /translation="MSIFQEPIFQWLSQYAYQPELVYTALLGMMLLSAIGLPLPEEVT
                     LVSVGVLAFMGANPQHFPPPYEGAPVVSMHTAALVAVIAVFGADFLIYMIGRMWGRKV
                     LQKPFMQKIISPEIMKKVEAWTKRYGAYACGIFRFTPGIRFPGHLACGMMHFPIWKFA
                     LIDGIAVLVSVPTQIYLLATYGEPIIKRLHQFKMVLAGIIGILLIAFLAKRIKNHFIK
                     EEQQSN"
     gene            complement(12989..14056)
                     /locus_tag="VIG33_01085"
     CDS             complement(12989..14056)
                     /locus_tag="VIG33_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269453.1"
                     /translation="MKKEKIQIGYEVIIEQGLVAKSIAKPIAIEFWKNPQDHAKWTQT
                     TPFKYMADALVGLASFKLNDFLKSPLMSPNMDFNFCAEAWRALDPHFELFQRTTARVN
                     ALKAQAPKTDPKLLESLEKSLFEIITNAQKPWGLDLKELFTINDAVAHFENKSGNHLI
                     YNFGLNFSKGLNEKLQQLFSCLFHLRSLIALDYNAHTQDATFEAVKVDAIFDYIPKPD
                     YIVHDALLYLNFKRLSQPFAQRKNDTHIDKLFVSPMQKAFEQYTHNAYFLIEQLPKSF
                     LNSLNHSELEEALYMIQTDWLLGSEAGVLFKIREELFGLEYGYDKIFWHDLEKKQNFK
                     PANLSICCEIDATDLFDHAAS"
     gene            14237..>15146
                     /locus_tag="VIG33_01090"
     CDS             14237..>15146
                     /locus_tag="VIG33_01090"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8269454.1"
                     /translation="MWIDRDISAALQKNKDAIQIIRGPRQCGKSSLILRLDPEFEELS
                     LDDPSLRELAQNDPELFLQQFSSKKLFIDEAQYAPNLFPSLKRRADLYKRESKSKPQT
                     ILRLTGSNQILMDRNVKESLAGRASFFDMNTLSVAEVLAAKKVTVQEILYRGGWPEVH
                     ANGVKDTKKFLDDYINSYVEKDIVLAAGIHKSREFLKFVKLLAGRVGEILDFSSFGSD
                     VGVDGKTIKDWISILERMHIIALVLPFSSNLSSRLIKSPKIYFIDTGLACRLQGWTSA
                     EPIVTSPQQGALFENLVFSEIYKLNIN"
BASE COUNT         4105 a         3148 c         3544 g         4349 t
ORIGIN      
        1 tctttgacga aatggaattt aatgcgggca ctgagccgca tgctgctcat tggctatggc
       61 agcactcata atcccgaggt tgctaaagag gtgatgaaag agcctggcct cgagcaatgg
      121 tacgcggatt ttaaccaatt cggaattgat ttgaaagctt ttgatcgacg ctcccagaac
      181 tcaggagttc gcagttttaa agaagcgaat tttttcacct acaagggcaa cggcgattct
      241 ttaatggatt ataatgagac cttcgatttt gtaagccttc tcgtttcagg cgggaaatct
      301 tctgcggatt ttattcgtga agatattctc aagtccggtt gtggcacgag cgacaaggat
      361 atttttgatt attattggat ggatgaagtc tgttttaaac ggacccttcg taagaaattt
      421 gctgtttatt ttaacaattt gccaggcatg gccagtgaag tttcagaaat gtcggaactg
      481 caatggaatg gtttttatgg gaatcttatg ggggcagcgc gggtttctgc cgccacgggg
      541 gggcgtgtgg aaacagcgga cttgcgcaca gctgtgatga tcttgcatta tgtcgaaagc
      601 ttaatgagtg tttacgatgg caataacgat ggaaagctca atgtcgaaga attgcgtatc
      661 gcttcctcac gatttttgaa tttcatgaaa acggtttccc ccattgatgc taattttatg
      721 gttgaggatt tctttttatt tttagttcat aaagggcaca agcccacatt gggagaatac
      781 acttatttcc aaggggaaaa ggcttttgat agccttaagg atgtgggccg tgataagatc
      841 cttcgcgtct tcaaagtatt gaaagatgag gcggcccatt aggcaagtcc tctaaggttt
      901 aaggtctagg ctctaaggat agatcttacg aaaggtctta tgaacaaaaa ctggttttta
      961 tttcttctgc tcttttcgtg cgcagccttt gcaggaaagt cgacttcctt tgaaaaggga
     1021 tgggctcggg gttatgttca gtccatcgat agtgccagaa ctatgaccgt gcttacagct
     1081 tattcggaat tcagcttggg tgaaatcctt gtcttgcaaa gccgtgatcc tagccaagat
     1141 attattggct ttgtgcaaat tgaaagcatc gaaacccaag gggacggaca gtatatttta
     1201 aaagcccgtt tggttcgcca ttcaaaattt gcaatggttc aactggggga tgttctttac
     1261 aagatggaat ttaacacctt tcatcgagag tatcatggca ctacggagtt gttaatgcga
     1321 aatgaggatc ccgaatcctc ttctagttat aaatctctga tcttcatggg gctgggagcg
     1381 ggggagaccg cgcaaacttt gcgtgagaat gaatttctgg tggatatatt tggctatttg
     1441 gactacggag tgaccgcaaa ccttactctt ggaactcttt tgcctgcgga tcttttttca
     1501 gctccgaacc ttcatgcaaa gtacagagtt tatcaatccg attcaagcgt gacttcactg
     1561 gggcttaatt actttcagtc acctgatact tcgagtggaa ttattaatat gagtttgtac
     1621 tgggactcgg tttcctctga aagcgtgatt gcccacacca atctttcctt ggctctggcc
     1681 acctttgaca aagcgaaaaa tctgacagct cttaaggggg ctgggaccag ttctttgcaa
     1741 tcgggttatg aatttattat ggataattgg aatcgtgtct tggtgggccc aagttataac
     1801 ttcgaaaaca aatccatcgg gggttatctg tcttatgttt ggatatggga tactttccat
     1861 ctcttagccg gtgtcagcac ggcggatatc tcgaatgtca tctacaacgt cgaaaagggt
     1921 tactacgtta atatcgatat gtactggcgc ttttaataaa agaaccaaga tgaaagagcg
     1981 aaaatgaaag aaagttttta tatctcctgc aatttgggtt tcgaagagac tttggctcag
     2041 gaaattcgcc agtgttggcc acaattaatt gaaatcaatg gacaagcgaa tcaaagccct
     2101 ttgccagagt tgactctaga aatgggaggc gtgctggtga aaacatcttt tgagcttggc
     2161 ttgcaattta atttttttct aaagaccgcc catcgtgttt tgtggcgaat tgcggaattt
     2221 aaagttcgag atttcccaaa actctttgag aaagttcaga aaataacttg gggaaattac
     2281 ctcaagggaa gaaaaataga gtgggtcgtt gcagccagta agtcccgttt gaataatgaa
     2341 aaacgtattg aagaaacttg cggcgaagcc ttcgcgaaag tagcggcgaa agttcagggt
     2401 gagtctctgg cccctcaggc agtttatgta cgcatctatg aggacctttg cactctgagc
     2461 ctcgattcca gcggtgagaa tctttataag cgggggtggg ggacgctgaa gggtgaagct
     2521 cctcttcgag agactctggc ggcattttta attcaggaaa tgatgcagga tgctcctccg
     2581 aataaaactc agcaggtgac ggtggtggat cccatgtgtg gctcagggac cttgattcta
     2641 gaagccgccg gtctttggca accggttttc gagaggaaat tctcttttct cgactggaaa
     2701 aatacgccaa aaatttttag aagttcttta ttaaaaaaga attataagct gctgcagaag
     2761 caagctcctt ttaaaagtta tctgggttac gatattgatg agaaagtgtt agccgcggcc
     2821 gaagaaaatc aaaagtcttt agagtcgaaa atggacgtca agaatctcaa gattcgtttt
     2881 gaaaaggaaa atctgtttaa aggcgaaaag aaaaatcttg gaccggtatg gtgtatcacc
     2941 aatccaccct acggcgagcg tctgcatgtt caaggacgag atagccggga tcaagaggct
     3001 ttttcttatc aggatttgct gcaaaggatg gctgataaat tttcagcgga gaagatcggc
     3061 gtccttttgc caaataagcg cagtgtaaaa agtctcaagg ctccagaggg ctataagaaa
     3121 agaaaagaaa ttccttttag taacgggggc cttgaggtcg tctatatgat ttatagaaaa
     3181 aaatagtccg gttattttac tattttgcct gtcgaagttc ggcgaacttg acccaagcgg
     3241 gacgagacct catcactgag agccaagatt tgatgttagg atagggatta agatctactt
     3301 gtagtcccat ggccatattc atcacggatc ctgccatgac atcggctacg gaataagtgt
     3361 ctgcaactaa atatttttta ttaatcagtt ctttttccag gacttcgaag agggcaggga
     3421 cttgttgctc acgtcgagca atcaaagcca aatcacgttt gtcctctgga acaaaaactt
     3481 tctgaatcaa aatatccact aaaggaggtt gagcttcggt catggaccag aaggtccact
     3541 ggttcacgag agctttatct tcagtgttta gtcccaacat agcaggttta tatttatcag
     3601 cgagataatg cacaatagcg gctgattccc aaagcacaaa atcgttgtca atgagacagg
     3661 gcactttgcc attgggatta agcttcagaa aacttgctga tttgtgttcc tttttgccca
     3721 tatcgagcgg cctaacttca tagggaagtc cgcactcttc aagcataagg aagcagcgac
     3781 cagcgctgct tctaggagag ccataaattt gaatcatgaa atcctcacgc gaatgaatgg
     3841 agttaatcaa ttaaattact gtggagcccc ttggtccacc catgcattga tgatatcgcg
     3901 ttgcgcttga ggtaacaagc cgcctattgg cattgggttc atcgcattat ttatacggga
     3961 tctgataatc gcacgggcgg tcttcgcagc gatgtaatta gttaagtcca gggctcctcc
     4021 aaggttagtc gcgttgtgac agccaacgca gctatttgca aaaatgcccc ctgcggccgt
     4081 aagttgtgaa tacttaactg tcgttcccat ggaaggaggg ggggcgttgc tggccgactt
     4141 caaactggaa aattcaaggg cgatcacatc cgaatttgct ggagtgagaa cggcatagcc
     4201 attggcataa ccaggagcca aattgacatc catcgtagac gaaacactca ggtcaatatt
     4261 actataggtc gtcacttcag tttgaagttg gccgttgatg taaagattga gagctctcga
     4321 ctgataggta ccggcattcg ccctctttaa gcgcaatgta gggtttctaa attcgtaacc
     4381 ctcagtgagt ttattaagaa cggctttacg gatatcaatg cccgcaatca aagggatttt
     4441 gacattgctt tcggtctcga gatcccattc gattcgaaca aaagttgtcg acagagtcgc
     4501 agcgacggtt ttatcgatgg ttttaaccac ggactgtaaa gaacctccgc cactctccga
     4561 aacgcaactt gcatattgaa cttgagcggt gctccaataa gattggagtt cgttcatccg
     4621 gtccgtgttt tgagaaccgg tataaggggg tttgtggcta ttattcgtgg cttgactgct
     4681 aattttatta acgccgatgg cataaaagga gttaaaagaa agctgtacgt ccgaactcgc
     4741 aaaagttcca ataccgggtc cgttcacgtg gcagttatta caggtctgag ataaaaatgg
     4801 atgataagtg ccttggtaga cctttagtaa agcgtcttca catgtttgcc ctgcggctcg
     4861 ggaaaataga acagaattgc cgctgggagc ttgctgtaaa acgaatcctt cgccacaatt
     4921 attaaaggca acattaaaga caatcaagag gagacatcct ccaaacaccc actggatatg
     4981 ttttaatctg tttatttttt ccaaaatagg cccttccccg cacatggcgt ttgagcagtc
     5041 gtttaagcca atttgataat ctgagagagc tgctcagtat caaaaacacg accggctatt
     5101 ttctcgaaca agtcattgcg tttatttgct tggcaccagt tcgcaaagac cgccgccgcc
     5161 accatctcag gatttcctcc agtgataaag cttgtatcgg cggactgatt gtcattgaat
     5221 tgaccgatct gataatcgct ggtggccggt cggccctttg gatcatagta aagcatatag
     5281 gctataccgt ttgagcctcg atcgctggtc cagggggcat ttctatttgg tgagtcctgg
     5341 caggagacgg aaccgtcaga aataacatag ataaaaacag gcttatttaa aactgcagcg
     5401 ctctgtaaaa ttcgcccgac agtggtgcca gcttcttggt ctttggcgtc gcccgttgtg
     5461 cgcgtattat tatgataatc aaatccgccc atctcaagac ttgaagctcc cgcttgaccg
     5521 gaaagagtat tgtagatcat ggaaccaaaa ataagctcgc gatcgttatt ggcggtagta
     5581 gcattcactc cccaaacttg agagaaggct gtgttttggc gaggatcaat cgctgaagca
     5641 ccctgcgcaa cgacatcaat atttttgatg cctgcacagt ccaatacctt tttcacaccg
     5701 ctggctcctt gaatggaagc caatttgcga gtttgaatgg tattgaggtt gctcattaat
     5761 ttcgccaaag aatttttttg ctcggggttc aaagcttttc caagagcgga actatagcct
     5821 atagacgtca gcagcgcacc atagtcattg accaccaaag gtgcgggggg agctatgata
     5881 gcggaagctt gatttattcc ggtgcggttg ttcacgcgcc ctaaattagg aaattttgtg
     5941 ccaatgagcc cggctttggc aatggcccca gagatatcaa acttattatt ggggctgtcg
     6001 ttttgtgaat cgcaggggat gccaataaag gctgtattgg ccagagtggg ggcggtggct
     6061 tgattccgta caccgacgag aaatttgctg ataagaactc cattattcat tccggcaaaa
     6121 ggaacatttc caaactccat ttcaagggga actttattat cgcccagacc cattttgcta
     6181 tagctggcaa tgggctcacg ctttgcattc atcgggacaa aattgctacc taaggcagca
     6241 ccacctgaca ggttcagggt tacgaaggga atgagtgaag cgggtaaagg gcaatccagc
     6301 cctgtttctg cttgggcttg agagcccaac aacaaagaga cccaattcgg aatggccagg
     6361 ctagcggcaa aaggaagaat tccagcagag agaaattctc gacgagtgac cggttttgca
     6421 tgccccgttt gcgcccacag aggaatcttt tttttatttt tacccatctg ctccccttag
     6481 agaaaacttt aatatgaaat agcgtccacg ctcgaaagca tggccgcgca agtggccaat
     6541 accaaatttc tgatggctac tggttttgtt ttatccgttt cagtaagcgc atttataaaa
     6601 tctgtcctaa attcttgcag caaagttaat tcttccaggg tttcattgcg cccccaaaag
     6661 gatcgggcca tgccgcgaat gctggtgtta agtccattgt cctccagccc tgaaactcca
     6721 gtggtaaagt caatgcttcc aaagaagctg cgagacgtga cgtcggtcat tgctttctct
     6781 tggattaaaa gactgttgca aatctccccg gccaggctcg tagaacctaa cattaaaggg
     6841 ccatttgcca tactcagatc attgccggcg gctaaggccc catagcgacc tttatattca
     6901 gccttaagag cagggctcag cgtcgttaca ttggttactt tcatcattga tgaaagtgtt
     6961 tgttccgcgg aaagaagagc atagggaaga cccaattgct gagagtctaa actttcactt
     7021 cctaaggaag cattttccgt gctctcttca tggggttggc taaagcggcc acagttgttg
     7081 aaaccaaaaa taatcacacc gataagggga acacataaaa tggcgacttt tgaaggctta
     7141 ttgagcatcc atcccccttt aatttcccag gcattctggt ttgccggcaa cagattcaaa
     7201 aagctttttc agttgatagc cagaggcttc gaactcatcg ccccactttt cgagtttgct
     7261 gcggtcctta gttgcatcca ccattttacg gcagactgtt tcaaaagcgc gttttgccat
     7321 gcattggctg aaacgacggg aatttgcgac gattgcgcca aagtctttca ctccttttcc
     7381 aattgcggaa tttccgcgcc attggaatag agaggcattg ctgggacgaa tggcattgtt
     7441 gacaaaggaa tcatccgtca taatatagcc agatggaaaa atggtgttat ttttattcat
     7501 tttgttcgcg actccgttgg catcggcagc gatgccaaaa tcaccccctc cgcgagcatt
     7561 gactgcggag tgcttgaggc ctttgccatc aaaatcccat ttagcgaaag cgcctcggaa
     7621 tccgtccata actgtatggc agcccttaca agaggtcaga tacttcatat ggtctccggc
     7681 cgggaagcgg tccacatcgc ggccgatacg tgcgtcggag gcttctgcat ccgcccatcc
     7741 ttcttttgga acacacatga attcacggaa ggtgtattcg agcaaacggc ggttggtgcc
     7801 catcaaagcg tgggcgccca tgaaagctct tgaagttaaa acaccggcag gatcaggatt
     7861 cggagctgtc gcgctcgcac tggtcgcgat catttgtcct tcaacccgaa ctaagtttgc
     7921 ggccagatcc acacgattgg attcaagatc agcgtagtga ttattcgaca gcaaaagatc
     7981 gttaatcaac tcagagcgaa ttcccgcacc gcttcccatg taataaaaat ttcctgtaag
     8041 aagttcccgc gcatctttgc cgtctcgagt gaccccaata aaatccgccg ccatatcatt
     8101 gagtggagtt ttaatggttt cctcacgtgt ggaaagcgcc aatgccatta acttcaccgt
     8161 gatattataa aaattcggat ctgcggtggc gatctgagca gctcccggca gatccccttt
     8221 gttgaggagg tcaaccattt gattgatcac gggactatcg ataggtaatt ttgcacctgt
     8281 taatcgctca tacatttttc gggctttttg ttcaactgcc gagggctcag ctgccattat
     8341 attgggtttc acttgagcat ggcaaaaagg tgcgaaggca ctgactatca ccaatggtaa
     8401 tagagagtaa gcaagcgaca tcaattcccc cagttcgaac attctatctt attgtgcctt
     8461 acaatagatt tacgaaaaat agaaacttgc acatttctta acttgagatt tgaagatcat
     8521 agaataacgg aaggggaggc gactgtctca ttgctggaaa aactgaggga gcgaaggagg
     8581 cagtcgatgt gatagaggac taaattgatt tagtgtttcg caagctttca agaattgatt
     8641 ttgagtcttg gttgagcgca cggatctctc ttgttaaagt ttctacttct gaatatcctt
     8701 tgaaaatctt attcgagctt tggctcctat tcataattgc aagtaattta tcgatttttt
     8761 tctttgcaat tgtggcggca gtttcattga cacccagagc tttgagaaaa ccaccggagt
     8821 cttccattga agtccctact tccgtcacaa tatcacttat ttcttttaac aaaatggacg
     8881 cttccgtctg catttgtcca gcattcataa gcacttgtgc ttgaattttc gtttccgtat
     8941 tttcatcccc aaaggcgtgg gcagagaatg aaaaactcaa agataaaaca agagttaacg
     9001 tattcatcag ggctttcaac atgggaacct ccaagtgcaa atagtttttt cacaggagtt
     9061 cctttgcgac attcgcgcca ggacgggaaa cgctttattt cacttatagg caaagagact
     9121 gtctaagttt tcaatttaag gcgctgattg cttctgtcca aaaaatcgac agctagagaa
     9181 cttcgtcggg acatcagcga caggaaccgc catttttgct ctgagtctaa tgcgagcgta
     9241 aagcggaaag tggtccgaga ctccctcttt ggtttctggc ttaaagcgtt ttggatagga
     9301 gccccagagg tgtatgggat cggagtgcac gatggtaatt gtttcaggct ctaacttgta
     9361 ggaggccgtt cccgactctc ccatattttt tgaaaacaat aaagcatcca ggaaggacca
     9421 ttctttacga tagttgtggg agccttcaca tttcttgcag cccaccagat gagaaaccag
     9481 agccacttct gagaagctat catgaaagaa acgagcttcg atttcttcat tatggctgat
     9541 gttaagatct ccgctggcga taaccatatt attggcggat tcttgaatca ttttagctaa
     9601 gaattccacg gcttgttcgc gccagtagcg gggattcgcc tgagatggaa aatgggcgcc
     9661 cagcagagtc agtgtttctt gattcggcaa ctttagtttt acctctaaaa ttccacggga
     9721 ttttcccatt cctattttat cgctgtcatt tttggccaca tacggaatct tatgaagctc
     9781 tggagttccg acgatgggga aacgtgaaag gagagcgaca tcaattcctc gctcatccga
     9841 gccttcaatt aaaacttggg tttgatagcc ggcttttgtt aagaactctt tattgaggcg
     9901 agtgagaatc cgctcatttt caacttcttc cataacgaga atatcgggac cttgcccatc
     9961 gacccctaat atgacttgcg ccaggttttt caactttgca tttactacat cctcgttcca
    10021 atccagcgtc agacattctt ttcgataatg gatctggttg atttttgcac agaccgcttt
    10081 gtgttctgga gtctgttttt gtaagagggg caaataggca taatcatcac gattttcgtc
    10141 atgaatggtg tcgaaaaggt tctcgacatt gaagttcatg acactgactt cgtcaggacg
    10201 gggagttttc tcgcggattt ccttggtttc catggtcgtt ttgggaccgg aagtggtgca
    10261 accaagcgtg aaaataaaga aagaagcagc aaacagcgta aagaagcaaa tgggtgaata
    10321 tttcataagt atcagaatga gagaagtttt ggatctgatc aagctctagt tctgttagga
    10381 attgattaat tttaaaggga agggttgtgt cttataattt tttatgccat ataggccggg
    10441 agttgagtga atgctccggg gaggccttat gcgtttttcg cgtttttgga tggttgtcag
    10501 cactgcattg ttcacaagct tcatagccag tgctcaaaca gctggcacca atatcctaga
    10561 ggtgagtccc ggcagtgaag ccccctcaaa atggggagtt tctttttata gtcttggcag
    10621 tatcgctcag aagcaaatcg aaacggggga tccttcctac ttcacctata actatattgg
    10681 tttgaattat aaaatttcta aatcaagacg tttcgccatc cgcccggttt ttaactatgt
    10741 ctcttcggga aaagataaat acgctaacga tgtaaagtcc gagatgacga cgggggattt
    10801 gcatattgtc tatgcggatt atgaaattgc gactcttggt gaagccaacg tttccaccag
    10861 tttcaaattt tacctgccca caagtcaagg gtcccaagac tcccagatga ttgtaaagtt
    10921 ccgtcctgag actttcattt ctatggaagt aaggcggcat gacattctca cttatgcttt
    10981 gaagccggat tttttcctgc aatctcgcgg ttcttatctt gatgctaatc gtaaagacag
    11041 atcgactcaa attgcgttga ttgagcatta tttggaatac agtgcaggtt taaataaaat
    11101 gttttcctta aagccttctg tgggcttcat agaaagttgg ttcaatcctt caactaataa
    11161 gaattttaat actcataaaa cagaggctaa aattgcctta ggccttgata tcaatctcat
    11221 gaagggtctg atgttcacgc tgagcgcgga aaatagagta ttgcttacag atcgcaaaga
    11281 tgcagtgact ttttttcgac ctgaagataa tgggctggtc ttaattacca gtgcttcgtt
    11341 actgtaaaat taaaatctag aaattgaagt gagtgcgcct cgagatcgat tttctcgaag
    11401 tcgtattttt tttgtgctcc atacaggata tctccacaga tcggggctcc tagggcagag
    11461 agctgagccc gaatctgatg ggtgcgaccg gttaggagtt cgacatgaac ttcctgttcg
    11521 ttcaaaagga catgatcctg aatatcaaac aatcgaagct tacattcttg ccagtgaggt
    11581 tggggttctt cgctgacttt tttaggggcg cggggagagg gttccatata gtgagtataa
    11641 agtccgtctt tgagcttcag accctcggct cgagcgcgat aaattttccg tacgcctcgt
    11701 tgagcaagca gatgattaaa taaaggtaaa aattctctat tttttgccag cacgacgagt
    11761 ccactcgtag gcacatccag gcgatgagtc acaaagagtt ctgtttgcaa ataagcagac
    11821 aagtacttat gcaagttttc taagcagttg tcgacactgg cgtgcatcgg gagtccagct
    11881 ggtttattca ccactacaaa attttcatct tgaaagagaa ttcgctcact ccagttatag
    11941 tcattagtgg gaaagcggcg tggtttactg tggatgcgaa tataggcgcc ttcaacaata
    12001 agacaattct cgtgaagacg ctggccttcg agatagaggc tacctaactt caaaagctca
    12061 tcaaagcttt ccgcggaaaa tgagaactca cttattaaaa tttcatttaa tagaccagac
    12121 ttagggctga ggatgtgttt aacaccatac tcaaatccgt tattatagtg attcgtttct
    12181 ctgttattgc tctgcatgac cttttgttgt gatagcacag cgtgacgatt atgttcaagt
    12241 ggtgcaataa aggccatatt agaatcatag gagcacgcgc tttgagtatt tttcaagagc
    12301 ctatattcca gtggttatcg caatatgcct atcagcctga actcgtttat acggcacttc
    12361 tggggatgat gctgctttcc gcaattggtt tgcctcttcc cgaagaagtg accttagtga
    12421 gcgttggggt tctggcattt atgggggcca acccccagca tttccctccg ccctatgagg
    12481 gggctcccgt cgttagcatg cacacagcgg cactggtcgc cgtgatagcg gtgtttggtg
    12541 ctgatttttt aatctatatg atcggacgca tgtgggggcg aaaagttttg cagaagcctt
    12601 tcatgcagaa aataatttcc cctgaaatca tgaaaaaagt agaggcttgg acaaaaagat
    12661 atggagccta tgcatgcggt atttttcgtt ttaccccggg gattcggttt ccgggtcact
    12721 tggcctgtgg catgatgcat tttcctattt ggaaattcgc tttgatcgat gggattgccg
    12781 tattggtcag cgtgccaacg caaatttatt tattggccac ctatggtgag cccattatca
    12841 aacgtcttca tcagtttaag atggttcttg cggggatcat tggtattctg ctgatcgcct
    12901 ttctcgcaaa aagaattaaa aatcacttca ttaaagaaga gcagcaaagc aattaaaaaa
    12961 tccaaagtct ccaatcagct aaaagcagct aagaagccgc gtgatcgaaa agatcggtgg
    13021 catcaatttc gcagcaaatg ctcaggttgg cgggtttgaa attttgcttc ttctcaagat
    13081 cgtgccagaa aattttatca taaccatatt ccaggccgaa aagctcttcg cgaatcttga
    13141 aaagaactcc ggcttcagaa cccaaaagcc aatctgtttg gatcatatag agggcctctt
    13201 caagttcgga gtgattcaaa gaatttaaga atgatttagg caattgctca attaagaaat
    13261 aagcattgtg agtgtattgt tcaaaagcct tttgcatagg ggatacaaaa agtttatcga
    13321 tatgggtgtc attttttctt tgcgcaaagg gttggctaag acgtttgaag ttcaaataca
    13381 gcaaagcatc gtgaacaata taatcaggct tggggatata gtcgaaaatg gcatccactt
    13441 tcaccgcttc aaaagtggcg tcttgggtgt gggcattgta atcaagggcg atcagcgaac
    13501 gcaaatggaa gaggcaagag aaaagttgct gaagtttttc attgagacct tttgaaaagt
    13561 tcaggccaaa attgtagatc aaatgattgc cggatttatt ttcaaaatgg gcaacggcat
    13621 cgttgatggt aaaaagctct ttaagatcca atccccatgg cttctgggcg ttggtgataa
    13681 tttcaaaaag agatttttcc aaggattcta aaagttttgg atccgtttta ggggcttggg
    13741 ctttgagtgc attcactcgg gctgtggtgc gctgaaaaag ttcaaagtga gggtccagag
    13801 cacgccaggc ttccgcgcaa aaattgaaat ccatgttggg ggacatcagg ggacttttta
    13861 agaagtcgtt caatttgaag ctggctaaac ccaccaaggc gtcggccata tatttaaagg
    13921 gagttgtttg cgtccatttg gcatggtcct gcggattttt ccaaaattca atggcgatcg
    13981 gtttggcgat ggatttcgca acgaggcctt gttcaataat aacttcataa ccaatttgga
    14041 tcttttcttt tttcattccc tctccataaa aacaaatata aaggactgtt tcttttatcg
    14101 gttgcaacag aatttgtgag agtcgggttt ttgtggcact cttaaaccat gaccaaaagc
    14161 cagtggatcc agaagctctc ctcctttggc aggagttcct tgacaaaatc ggagtataac
    14221 tccataattg ccaaatatgt ggatagacag ggacatttca gcagctcttc agaaaaacaa
    14281 ggacgctatt cagatcatcc gaggtccacg tcaatgtggc aaatcaagtc ttattttaag
    14341 gcttgatccc gaatttgaag agctttcttt agatgaccct tccctgaggg agctggcaca
    14401 aaatgatcct gaattgtttc tccagcaatt cagttctaaa aaacttttta ttgatgaagc
    14461 gcaatatgct cctaaccttt tcccttccct caagcgcaga gccgatcttt acaagaggga
    14521 atcaaaaagt aagcctcaaa caattcttag attgacaggt tccaatcaaa ttttaatgga
    14581 tcgaaatgtt aaagaaagtt tagcgggccg agctagtttt tttgatatga ataccttgag
    14641 tgtggcagaa gtcttagcgg ccaaaaaggt cactgtccaa gagattcttt ataggggagg
    14701 atggcctgag gttcatgcaa acggagtaaa agatactaaa aaatttcttg atgattacat
    14761 caatagttac gttgaaaaag acattgttct tgctgcagga attcataaaa gccgcgaatt
    14821 tttaaaattc gtgaaacttc ttgctggccg cgtgggcgag attttggact tttcaagttt
    14881 tggttcagat gtgggtgtcg atggtaaaac tataaaagac tggatttcaa ttttagagcg
    14941 aatgcatatt attgctcttg ttttgccatt ttcatcaaat ctgtcatcac gtcttattaa
    15001 atcccccaaa atatatttta tcgatacggg tttagcttgt cgacttcaag gttggacttc
    15061 cgctgagccc atcgtaactt cacctcagca aggggcttta tttgaaaatc tcgttttttc
    15121 tgaaatttat aaactcaata taaatt
//
LOCUS       DATBTE010000031         3564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2121106, whole genome shotgun sequence.
ACCESSION   DATBTE010000031 DATBTE010000000
VERSION     DATBTE010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2121106"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..167
                     /locus_tag="VIG33_01095"
     CDS             30..167
                     /locus_tag="VIG33_01095"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEY8269455.1"
                     /translation="MGLHKGQVIQLLGRAPLQGPLLIQFGTSFIALRAEEAQCTIIQV
                     L"
     gene            143..2077
                     /locus_tag="VIG33_01100"
     CDS             143..2077
                     /locus_tag="VIG33_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEY8269456.1"
                     /translation="MHHNSSFVKHLSVALVGAPNCGKTTLYNWLTGSNFKTVNYPGAT
                     VEYSLGRLAPHLRIGEDEKLLVMDTPGTYSLHPKSADEEVTLKALYSNPEPDLRFGRV
                     DFIIVVVDGTQMGRHLQLVQQVRETGFPFVVALTMVDLLKKQNIQISLSALEKEFQCS
                     VIPFEGLLGGGLKELVTAVHGLKPVAEVHKPVIWDFETSEKKIQKCQILASKVMNQTT
                     QEMAQRVQGLVKNTRRLDQILLHPILGLILFFLIMGTLFTSIFWVAQPLMDSVDGAFS
                     ALANLVHSLDDKALGMDFLANGIVASFGAVLVFVPQIFILFVGIGVLESTGYLARAAT
                     LIDKPFSMLGMSGRSFVPVLSGFACAVPAMIATRNISSARDRWITNFIVPLLTCSARL
                     PVYALFLAFVFKDEPAWKAGLWMAILYFGSALIGATAAAIINRFLPQGQNAFFMMELP
                     LYRRPRIRVLLRQSLTRTLSYVKRAGPPIFIFASLMWVATTFPHYDMENAQQKLEKSY
                     AGRMGHWIEPLVHPMGVDWRVGVGLISAFAAREVFVSSLAVTFNITDQNADSAQQSLL
                     TQMGTAVNSEGNKIFTIASIAGLIVFFMIALQCMSTVVVSAREMGSYKFALTQLIVFN
                     LAAYFLAVGVFQSLKALGFG"
     gene            complement(2074..>3564)
                     /locus_tag="VIG33_01105"
     CDS             complement(2074..>3564)
                     /locus_tag="VIG33_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY8269457.1"
                     /translation="AICICENLIAKLSQKLAMNPRKMRLIRNPVDSQNLNFVEKLSQE
                     QKIAWVGRFTGPKGERAREFIEKVIPVLLQKFPKLEIEVIGGNPQLLGNVTLTRIGSL
                     QEKYPKRLQVHTHLENLDQKLETFQMVMAAGRVAISSLMRGIPTYAIGEYSAEGFVSL
                     ENLSQAMNSNFGDIGADGKATSPIDFSKMTQELMAFLENPLLRTFAEHHSERQSERYK
                     IREIVFRNYGQESVCKKIFNTYKSAYFLKNYSRHIPILMYHKVPEQKLESRHRIFVSK
                     DIFESHLQFFKNHGFTTLTFQDLEEYRSGAKSFESFPRKPLILTFDDGYVDNLTNAAP
                     LLKKYQMKAVIFLLADHRLQTNSWDNDEQEPPQSLMSLEQKKELLNFDYEIGCHGFRH
                     RKITEMTDAEAQHELSDSKKVLEKDFGPISTFAFTYGITSAQAAEFAEEAGYNFAVNT
                     DTGGLHLEENPYSIFRVNIFPEDGPAQLRKKTSSSYRKYFHWKRGR"
BASE COUNT          837 a          756 c          911 g         1060 t
ORIGIN      
        1 ctccggccat ctgtgagcgt ctccatgaaa tgggattgca taagggacag gtgatccaac
       61 ttctcggtcg ggcgccgctt caagggccgc ttcttattca attcggcaca agctttatcg
      121 ccctgagagc agaggaggcc caatgcacca taattcaagt tttgtaaaac atctctctgt
      181 ggctctcgtg ggcgctccga attgtggcaa aaccacgctt tacaactggc tgacgggatc
      241 taactttaaa accgtcaatt atcctggagc cacggtggag tattctttag gaaggcttgc
      301 gccccactta agaatagggg aggacgagaa gcttttagtg atggacactc cgggtaccta
      361 tagtctgcat ccgaaaagtg ctgatgaaga agtgactctg aaagctttgt actctaatcc
      421 agagcctgac ttaaggtttg gccgtgtgga ttttatcatc gtggtggtgg atggaaccca
      481 aatgggccgt cacttgcaat tggttcagca ggtgagagaa actggatttc ctttcgtcgt
      541 cgccctcacg atggtggatc ttttaaaaaa acaaaatatc caaatttctt taagcgcctt
      601 ggaaaaggaa tttcaatgtt cggtgattcc ttttgaaggc ttgctcggtg gtggcttaaa
      661 agagctagtg accgcagtgc atgggcttaa accggttgca gaagtgcata agcccgtgat
      721 ttgggatttt gagacgagtg aaaagaagat tcagaaatgt caaattttag cctcgaaagt
      781 gatgaatcaa accacccagg agatggcgca aagagttcaa ggcttggtga agaacacgcg
      841 acgcctggat cagattttat tgcatcccat tttgggtttg attttatttt ttttaattat
      901 ggggacgctc tttaccagta tattttgggt ggcccaacct ttgatggatt cggtggatgg
      961 ggcttttagc gctctcgcta acttagtcca ttctttagac gataaagctc tggggatgga
     1021 ctttttggcc aacggaatcg tggcgagctt tggtgcggtc ttggtctttg ttccacagat
     1081 ttttatattg tttgttggca ttggtgtttt agaaagcaca ggctatcttg cccgggccgc
     1141 gaccttgatt gacaaacctt tttctatgtt gggaatgagt ggacgttctt ttgtgcctgt
     1201 cctttctggt tttgcctgtg ccgtgccggc aatgattgcc actcgcaata tttcttccgc
     1261 acgagatcgt tggattacta attttattgt gccattactg acctgttccg cgcgtttgcc
     1321 ggtttatgcg ctctttctgg cttttgtttt taaggatgag cccgcttgga aggcgggcct
     1381 ttggatggcg attctctact ttgggtccgc tctcattggc gccactgcgg cggcgattat
     1441 caatcggttt ttgcctcagg gacaaaatgc cttctttatg atggagttgc ctctttatcg
     1501 ccgtcctcga attcgagttt tgctgcgcca gtctctcacg cggacactgt cttatgtaaa
     1561 aagggcggga cctcctattt ttatttttgc gagtcttatg tgggtcgcca ccacctttcc
     1621 tcattatgat atggaaaatg ctcagcaaaa acttgagaaa agttacgccg gcaggatggg
     1681 gcactggatt gagccattgg tgcatccgat gggggttgat tggcgagtcg gagtgggctt
     1741 gatctccgcc tttgcggccc gtgaggtttt tgtttcttcc ttagcggtga cctttaacat
     1801 cactgatcaa aatgctgatt ccgcccagca gagtttgctc acacaaatgg gcacggctgt
     1861 gaactccgag gggaataaaa tctttacgat cgcgagcatt gccgggctga ttgtcttttt
     1921 tatgatcgcg ctccaatgca tgtcgaccgt ggtggtctct gcccgcgaaa tgggatccta
     1981 taagttcgcg ttgactcaat tgatagtatt taatttggcg gcgtattttc tagcagtggg
     2041 agtttttcaa agcctcaaag ccttgggttt tggctagcgg ccgcgtttcc agtggaaata
     2101 cttacggtac gaagaagagg ttttcttacg aagttgagcc ggtccgtctt cggggaaaat
     2161 attaacgcga aaaatggagt aggggttttc ttcaagatgc agaccgccgg tatcggtgtt
     2221 gacggcaaaa ttgtagccgg cctcttccgc aaactctgcg gcctgcgcac tagtaattcc
     2281 gtaggtgaaa gcgaaagtgg agatagggcc gaaatccttt tcgagcactt ttttggaatc
     2341 agaaagttca tgctgagctt cggcgtcagt catttcagtg atcttacgat gccgaaaacc
     2401 atggcagcca atttcgtaat caaaattaag cagttctttt ttctgttcca agctcattag
     2461 agactgcgga ggctcctgct cgtcgttatc ccaagaattc gtttgaagtc gatggtccgc
     2521 taaaaggaaa atcaccgctt tcatctggta ttttttaagc aggggggcgg cattggtgag
     2581 attgtcgaca taaccatcat caaaggtcag gatcagtggt ttccgtggaa aactctcaaa
     2641 acttttggcc cctgaacggt attcttctaa atcttgaaaa gtgagggtgg taaaaccgtg
     2701 gtttttaaaa aactgcaagt gagattcgaa aatgtctttg cttacaaaaa tgcggtgacg
     2761 gctctctaat ttttgctcgg gcactttgtg atacatcaag ataggaatgt gccgagagta
     2821 attttttagg aagtaagctg atttgtaagt attaaaaatt ttcttgcaga cgctttcctg
     2881 accgtagttt ctaaaaacga tctcacggat tttataacgt tcagactgac gttcggagtg
     2941 atgttcagcg aaagtcctaa gaaggggatt ttccaggaaa gccatcaact cttgagtcat
     3001 tttagaaaaa tcaatagggc ttgtggcttt gccgtcggcc ccgatatccc caaagttgct
     3061 gttcatggcc tgggacaggt tttccaagct gacgaagcct tcggcggagt attcgccaat
     3121 ggcataggtg gggattccac gcatgaggga gctgatggca acgcgtcccg cggccatgac
     3181 catctgaaaa gtttccaact tttgatcaag attttccaga tgagtatgga cctgaaggcg
     3241 ttttggatat ttctcttgga gggacccgat ccgagtcagg gtgacattcc ctagaagttg
     3301 ggggttgcct ccgataactt caatttccag tttcggaaat ttttgtaaaa ggaccggaat
     3361 tactttttca ataaattctc gcgcccgttc ccctttaggg ccggtgaagc gccccaccca
     3421 agcgattttt tgttcttgag agagtttttc tacaaaattc agattttggg aatccacagg
     3481 attacgaata aggcgcatct ttcttggatt catcgccaac ttttgcgaaa gtttggcaat
     3541 cagattttca cagatgcaaa tggc
//
LOCUS       DATBTE010000032        22827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1028502, whole genome shotgun sequence.
ACCESSION   DATBTE010000032 DATBTE010000000
VERSION     DATBTE010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 22827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22827
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028502"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VIG33_01110"
     CDS             <1..812
                     /locus_tag="VIG33_01110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8269458.1"
                     /translation="RVRNPIINVRLFLNPLVQNGVMLMGCLGVFLYAIVFILPIFVGR
                     VFHYDATQIGSLFIPGSMLTAVMMPFIGKQMVSGTNPKFLIAIGFISLEVSLWMMSGL
                     SPLSSRGDILNILFIRGFGMAFLFVPINSSILGQFKGADLGQVSGLLNLFRQLGGSIG
                     VAMVGTLLVKNTYQNYGDLSNNVSLLNPATQAAYYPSLKGMAMKMSDAVGFNQGAEAA
                     IKSIWGRVQNQAFMMSFLQLVFILMFVVAVGFIPLYRIKLKSGPTKIMDAH"
     gene            complement(818..1009)
                     /locus_tag="VIG33_01115"
     CDS             complement(818..1009)
                     /locus_tag="VIG33_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269459.1"
                     /translation="MEPQVLIIIGAIAISFISILVYAILAIFYPEWVGITGKVARQAE
                     ESHEEGKETGGHVFDKLDK"
     gene            complement(1098..2504)
                     /gene="dbpA"
                     /locus_tag="VIG33_01120"
     CDS             complement(1098..2504)
                     /gene="dbpA"
                     /locus_tag="VIG33_01120"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HEY8269460.1"
                     /translation="MNSTDLSLDFSSLPLSADLLTVVQELGYEKLTPIQAQSIPHLLA
                     GKDLVGQAKTGSGKTAAFALPILNKIDLQERSLQALILCPARELASQVVTETRKLGRR
                     HDGLQVLAITGGVPSRPQITALESGLHIAVGTPGRVLDLIQRGRMDLSKIKTLVLDEA
                     DKMFEMGFEDEIKAILKELPRTRQTAFFSATFPETIQMLSNKHQKNPIKVTIENTQES
                     APQIEQFVYAAEPEDKISTLMRVLQQHPSNSTIVFCNMKVDVNELAKILAEQDISSAC
                     LHGDLDQRERDRVMAMFRNGSHRILIATDVAARGLDIENLELVINFDLPLQPDIYVHR
                     IGRTGRAGRSGVAVSLATSREALRVVEIEKFTGKKFERRILGFKNQHGLAKDLRESAM
                     KTISISGGRKDKLRPGDILGALTGEAGGLSADKVGKIEIQDRSSYVAITASVADIAFE
                     RLRQGRIKGKKFQVKFMK"
     gene            complement(2883..3356)
                     /locus_tag="VIG33_01125"
     CDS             complement(2883..3356)
                     /locus_tag="VIG33_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269461.1"
                     /translation="MRLIFALTFGLSWSCASWAAVLHFYHNPRVPQALFHVALEYQGH
                     LFEADPTFGGRSEPMAQVKKQGDLRIKIADGLINEDALEAELGRAFDFNFEWNNNKTY
                     CAKLVGKALNMKPSPMNFAGTHYAIVHPEWLQRHDLGLSPDQIYEFALGHLPGLW"
     gene            complement(3371..4972)
                     /locus_tag="VIG33_01130"
     CDS             complement(3371..4972)
                     /locus_tag="VIG33_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY8269462.1"
                     /translation="MATLIDHIENAFDLSYTHFPRCPKTQIKTIGILGGGTAGYLTAL
                     ALQKLHPQIKTTVIESSKIPVIGVGESTTTEIVAFLHRTLEIDPLEFFQKVEPTLKLG
                     IKFDWGCPGDYKFNFNFFASHQYESYFYENSTANSSWPSVLINENKIPVIRNDDGSIT
                     SFLGSIPFSYHIDNKKLIQFLHAQLLERDIEIIDGEVNQVNFDEKQFVSSLQTVEGHS
                     LSYDLYIDCSGFNSRLLGKALGTEFIPFTDTLITDKAITFEIPHANTINCYTSVITMN
                     NGWCWNIPMREENHYGYVHSSKYCDEATAVAEARAKFGPFEKYKVVNFRTGRHAQAWN
                     KNVFSIGNAYGFLEPLESTAIQTAMLSIMTLCRLMPNSTSDYSSIAGINKEIAANWDS
                     FRWFLGIHYKFNKQLETPFWKYCNEHTDIGNAQMIIDLFNQRPPLSAGNFGTGSPYTA
                     LEALVFNSYSYDSLLFGQKQVAKPLAPPEMTKEEYLSKLESYKFLTDRALSLYELFKN
                     DHLIKDGLLEHLFTDTDTWIVDTEA"
     gene            5091..5525
                     /locus_tag="VIG33_01135"
     CDS             5091..5525
                     /locus_tag="VIG33_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8269463.1"
                     /translation="MSDIIEKSITVKASVDEIWRSLTDSDELENWWGDGIALEPRVGG
                     SFREPWEDDDGKIQLASGKVTGVKVKKEISFTWREKDWPKEALTQCTFAIEDKGKVRV
                     LTVKHAGWECFPLEARTKLFKDFQVGWGYHMKELKAYLDDEG"
     gene            5662..5859
                     /locus_tag="VIG33_01140"
     CDS             5662..5859
                     /locus_tag="VIG33_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002832300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEY8269464.1"
                     /translation="MSTGTVKFFNASKGFGFITPDNGADLFFHISEVQGQEPRDGDKV
                     EFEVGQGKKGPCAVNVKVKGN"
     gene            5940..6596
                     /locus_tag="VIG33_01145"
     CDS             5940..6596
                     /locus_tag="VIG33_01145"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HEY8269465.1"
                     /translation="MTRSPISSSIYRSALSRKRKTKDPCPICYLNRSLCICDSIPQLD
                     LQTKLCIVVHHRELKRTTNTGRLALKALINSEMRVRGESREALDLSDLLTPEYRSLLF
                     YPADDAVDLTPEFVAEDPRPIQLIVPDGNWRQASKVHYRHHELKDLPRVMIKARNLAK
                     LHMREEHMSEGMATLEAIACALGVIEGEDVKDTLMKLYNTKLTATLQGRGVPIGENDF
                     "
     gene            complement(6574..7977)
                     /gene="hflX"
                     /locus_tag="VIG33_01150"
     CDS             complement(6574..7977)
                     /gene="hflX"
                     /locus_tag="VIG33_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY8269466.1"
                     /translation="MNSPTHAAPLKALLVGIQLPKTSDQETQGSLAELARLVTTLGYD
                     VIGQTSQRRPSERSPSVLGDGKLRELAEWTGGTGVIGPLVVKKKHKAALKFEKNQTSE
                     MEKDDDEVDPENEDFTPHKAPKERAQIVIFDCDLSPSQLRNLESATGVQVLDRTGVII
                     EIFSRHARTRAARLQVEIARLTYVAPRLRETGGGEDRGGGGVGGKGAGETSVELDRRK
                     IRDRIKELKEELNSIAQEHDTRRARRGQEISVALVGYTNAGKSSLMRALTGSEVLVAD
                     KLFATLDTTVRLLYPESVPKILISDTVGFIKKLPHDLVASFKSTLDEALSASLLLYVV
                     DSSDPTFRSQLEVTRSVLNEVGAGEIQSLLVLNKVDRLSAEELASLKAEFPKAVFLSA
                     RSPEDVAALRLQLISYFETDMVDEEILIPYDVQGAIGEIRSKMRVLSESYDGKGVRLK
                     VRSHPENIAKIKSRFRL"
     gene            complement(8033..9157)
                     /locus_tag="VIG33_01155"
     CDS             complement(8033..9157)
                     /locus_tag="VIG33_01155"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8269467.1"
                     /translation="MGPRQTGKSTLIRSQFPQALLVDLLDPEFFRDLQKSPNFLREII
                     SGSKNNIVIIDEIQKIPELLDVVHSLIESNKKLRFILTGSSARKIRNQGKNLLGGRAY
                     PLSLHPITSVEYLSASATSQPQNLHRLIEAGGLPQVLTTKSPQRLLKAYVGIYLQEEI
                     KAEGYVRNLGDFSKFLDVAALSNTEQIDYAGIARDVQLSPRTVSAYYQILQDTLTGFL
                     LESFKETKSRKAVITPKFYFFDVGISNYLTGREKLSVGTPEYGKALEHFIFTELKAYQ
                     DYSEKAFELYYWRSTSQMEVDFIIKTKSKKLICIEVKGSQHIDKTDLKGLKAFEEDFK
                     ISKKIVVCNERLPRLFEGVEVLPYANFCHQLWADQIIQIP"
     gene            complement(9308..10489)
                     /locus_tag="VIG33_01160"
     CDS             complement(9308..10489)
                     /locus_tag="VIG33_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEY8269468.1"
                     /translation="MSAILFGLLCFIFVIMSVGSLAALFLFLLEQTSQFFLSHDHLVL
                     LLPLLGIVTGFTYWLFGKKLPTTKKILHGVEDQHALIPFLTALLIFFFTVVSQLVGAS
                     TGRESSAVQFGAALGDFWREIFDKKLKPLRFSRQAFIRSGLAAGFGAVFGVPWAGTLF
                     ALEATPSRRWSLRYLPLCWISSFGAHWIALWWGAHHKVYPVFPALPWNLLLIAKWSCL
                     GIAFGVLAKVFVVLMQYSEKTIFGRISWPWLRPFVGGLAVAALTLMMKETRYNGLGVP
                     LIDAALTSGVQTFDFAWKSLFTILSSASGLKGGEVTPLMAIGATFGVTISHWVALPSL
                     YAASLGLVGVFASASHIPWTGAVMAWEFFGFEAFLPTFIICWLGRRVLGLHGLFSMKE
                     P"
     gene            10538..11608
                     /locus_tag="VIG33_01165"
     CDS             10538..11608
                     /locus_tag="VIG33_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HEY8269469.1"
                     /translation="MFKGLIGYFTGSIVATILGMILAVFVGYYYSGTTAGALQALFIA
                     FILAVLEVSLSFDNAIVNALILKEMTPVWRQRFITWGMLVAVFGMRLIFPLAIVSVVA
                     KIDPWSALLMATFKPDQYAQLMLSAHLGVSAFGGSFLLMVTLRYFYDETKDVHWIAMI
                     ERPLSALGKVEAIEIGLTLLTLMGLSSFFPENKDQLTFLIAGIAGLMTYIVVDGIGGW
                     LELSQEGMKDLQKASAGMFVYLEVLDASFSFDGVVGAFAITHNLFIIMIGLSIGAFFV
                     RSLTILFVEKETLNKFLYLEHGAFYAIGSLAMIMLLDPFLHIPEWVTGLLGGFVILIA
                     LYWSIRFPEAPSRVRHQGSSEP"
     gene            complement(11634..12356)
                     /locus_tag="VIG33_01170"
     CDS             complement(11634..12356)
                     /locus_tag="VIG33_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8269470.1"
                     /translation="MSTARTVYSMRIEKIIDHTPTVRELILKTESPETFQYRAGQFVM
                     LHVPVPDAKPALRAYSIASDEREKTGFRLLFKFVETGLASTYIWSLKGDETLKFTGPF
                     GKVFFQEPPTEQIVFLNTGTGISQHLSYLVSKKDLFPHLQYRLLFGVRSEKDIYLKEE
                     LDALAAEFPNFKYEFVLSRPQENWKGKKGYVQHFLKEFNYQSTPTTFYLCGNGGMIKE
                     VKHQLLEVDGFDKTRVWSEAFD"
     gene            12485..14599
                     /locus_tag="VIG33_01175"
     CDS             12485..14599
                     /locus_tag="VIG33_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269471.1"
                     /translation="MKPLKVCGPHTRNIGKWKSSLVFLICWASFSYNVRANEEALNTE
                     APSSAPASSPTPTPASTGEKKNPRLILKTARNDQYTKPLYFESMDNLLPNVEFEYDLA
                     TGEGKSLRVGDTVIDEKSFFFAMVPVSKFHAKMSSLLDAKEVDKTALVMGWPEALQSG
                     GTLEMIAHSGKVVWKEEITEQKRDLWKDKLEFWKKGLEGKGVKTAILSKGSVFSTQFG
                     ILDIKSQGMRPYSESFRFCLTQTQGREQSRLCSQRYITRLRGNQLILKKSKSMVQPRV
                     LLQSQAAELKQSLPVPRDTPTSFFAELASGETYEFIATPNKLNLMDVTDTKTPEKIRI
                     MAWGARPTTPSTIINPDSYSSVTRALGFEATIGDKRKFWEALISSEDPKIYLPGQGGG
                     LFTQRFELAETPRANARPYLELHTPTGTYQDGVKLFGKKMPDVKITSSENSIEVDDND
                     PSAFLWRFKAKESGEINRSYLNLEHQGKEYKTFYELYKGFPREISLRLSGLIQASGQS
                     VFMGETAYNQWFENLLGWTNYWVGRQRWGISAKYFKSLTKLTVNAAKGTTADLTVMNV
                     DLKYRLVPGLWGRDETLGAMLDYQKIDFDKISAPMLGVGAFWARSMPKVFDDFFNFLP
                     LMQYPKWVDMEFIYYPLSMSSRVTLESNFALNFHGKVLWTKNWFGEAGFGIKRYAIVD
                     AQLRQKAALDTFYGTVGVGLNF"
     gene            complement(14608..15282)
                     /locus_tag="VIG33_01180"
     CDS             complement(14608..15282)
                     /locus_tag="VIG33_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269472.1"
                     /translation="MIKFTFLASLVISINAQAQQQIHVEKIKGNRAVVEFSGSLIPGK
                     TYSLSINKTEPPEETAGGKRQYIIGGSLGFNSTSYTVPSLNTTLKNSDINVFIRFGWN
                     KESFEFGPIFGYKNIDSDYTDRHYSSFSGGAFADYNLTANRLGETKIFGITSEGIVGS
                     LTPKTGDGGSTLEFFGGGFFKWFGITDSTALRFDMGYSYIKTTAGASSDTLQGLVLRG
                     GIATYF"
     gene            15512..16171
                     /locus_tag="VIG33_01185"
     CDS             15512..16171
                     /locus_tag="VIG33_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY8269473.1"
                     /translation="MSSQSASSWGDIPSWAYDAAQALMQSLKVVDPVTYAHCLRVGEM
                     SRKLARDASLNEYEQKLAEFAGIFHDIGKIGVDQEIIGKPGKLEPREMDIMKNHPCMS
                     EQIVRPLATHSFFRELLSPIRSHHERMDGEGYPDRLVGDKISVLARVILVVDTYDAMS
                     QTRAYRKGLPDEIVYAELKRCSGTQFDPQLVRIFLQAHKTWNALETEKESFQQFVKKL
                     G"
     gene            complement(16189..16998)
                     /locus_tag="VIG33_01190"
     CDS             complement(16189..16998)
                     /locus_tag="VIG33_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1338 domain-containing protein"
                     /protein_id="HEY8269474.1"
                     /translation="MNAQMPIHKLLDIMWKDYCEMNPAAKKIYDLLTAEGETVVNDHI
                     ALRTFNHPRLGIKSLARQIEKLGYVMTANDYHFVDKKLYARHWQHPDESLPKIFISEL
                     QLEKTSPFLRDTINTLTNSVPDSVIQSETFALCGRPWDLSYKLYNELAKESEYASWVA
                     AHGFRPNHFTVFINALKKYNDISALNKFLALKGFALNKSGGEVKGTPAEFLEQSSTMA
                     SEIPVKFSDGTFSIPGCYYEFAKRYPMPNGKLYQGFIAASADKIFESTNKM"
     gene            17127..18146
                     /locus_tag="VIG33_01195"
     CDS             17127..18146
                     /locus_tag="VIG33_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY8269475.1"
                     /translation="MLLEIQQVEKVIHELGPLVRSEVLAYSRTHSLKLPIYKLSFGSE
                     DPQAPVVGLVGGVHGLERIGSQVCVSLLHSFSELLLWDEVIKESLKHLRVFFIPVVNP
                     IGILKKTRSNPRGVDLMRNAPIEGEHPPRFLGGHRYSSKIPWYRGVEGAPMEVESRAL
                     VEAVQKEIFQSPLAITVDFHSGFGIQDQIWFPYAKTTKAYPEIGLAHAFKSLFDRTFP
                     HHFYKIEPQALNYTTHGDVWDYLYDKYREQYALNGSAKGVYLPLCLEMGSWMWVKKNP
                     LQLFNLQGHYNPIKSHRMKRTLRRHNTFFEFTLRAALSPQSWAYLSEEQRVKHETLAT
                     ELWYA"
     gene            18240..19748
                     /locus_tag="VIG33_01200"
     CDS             18240..19748
                     /locus_tag="VIG33_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8269476.1"
                     /translation="MKEKRFPFRTVKISKTTARRGNLKRRFLWLTVYLLEMMTFAEKS
                     VADLRLRTEPSSSSISFVSEVLKEERTQHRVYGQLRIEGMKYLTEISETPQLTYSQFL
                     SGQLSYVGETDWLENAVDVAAGTFFSQNQSHFVVHELYTSPRTQGYRVYVGRKKNGWS
                     EADHEWNLGVWQPYFELDALRPQEQGLTGLFLDVNNTEWQVLGMVTPIFIPTLGPDIR
                     EEDGGLVADSRWYHAPSRQADLITNKPTPITYKLSIPEAAKLAAHGGYAFMGRVGNKD
                     KGFWAVSSYGYMPVNKLLLKRNIKYQINQDVGVIVSPDLNYHSVFSTDLGYTQGNARV
                     TFSFLQDNPEDKLPDQDWVLQKIYPLRAYTAGIEWTIPKFFSRSISLQLDYLRVDGGE
                     IEDIGSDGAVDDLNLFDQRVKFTDAVKVKMQGELARIYRHPLRVHFSYLYDKEQKGSM
                     VNTEFMFYPKQELALLLGADFLGTAENTPKTSGFLNQYRANDRIYGGMTYVF"
     gene            19738..22812
                     /locus_tag="VIG33_01205"
     CDS             19738..22812
                     /locus_tag="VIG33_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269477.1"
                     /translation="MFFKRNLCYLLSAILMMTSCTPKVGEAPPQVEQQKLNKTQCLSG
                     LQSVVESFFAGEATDAAVTSGWDCASTAIKTFKKYVYGRTGDRFEATELSDFLKKNFL
                     EASSPEITPALREEFMRIKQLFLGGNINYVTRAELDKILSILGNLQDISLRLNPYMKL
                     ITQKWTIAGSTHLQNDILYFEKASDEIQDAARALANIILENNQSYELDHFATFLREFS
                     KFSQRAWPLAEEIERDMPVVKKIKKAISGGDPDSIGPADWRSFVLLGARGYLQYLRYY
                     YFIKSAAEMGSSIRLGYLARSLEDLLGAFQDLVEQKPKDPVCGTSNSSCISKQEIDDI
                     LKTFSDIWQDFHTSDKLVDEVMKLKKIYFGGSDGTITSRDFERGKNKVGKLKFVVEKF
                     LPYFQVYSLEWDRANYDTASAEQYFNEAQLSLQGSGESFGALLEESYNLENLASLLSE
                     VARLYPLQDPSKDPNSHPALVMKKMVPLVKDMKNIIFSDGNGLVKKNQWSLFLKFSAN
                     FYNTYLFHYYFLKQETYGTAPFLEAFQKITDKVLNIAKDVVLLKKNQTLPIDEIKTLA
                     KDMSSVGYIPPGLAPDSIDQLVKVVLNRVLWPAELRLNGTIPNGITPVSIENTRLEIK
                     IWYETEKYLYSLTASPLNPADLQAQVAKKLKDAKISAELRTGLSEVSLMLTDGVPQPI
                     DSEGHLIITNIGKLTYNTVSISRLNINRFAGRVFIRAATTDLNRLKSYEGIDLKEAQE
                     FFDLIRPVVVELGLLEEKNTVFMQSRFREANIFSAHSNGDAYVNFQEMTDMVGMILSG
                     IAVNDMFRKDIMAACGPVKSQDETYVQESCIRKIYIDKTASYMTATPEYIKYFKQLQP
                     DEYSGFLANILKAAGHIPNNDENVNMLDADLVPHVFQYLEMTLAKFDANKNGVIDLDE
                     AKVAYPSFKGILMELTKDQTLIKEKDLLALFTYILHYGKPPDGVSDYLFKWLPWKSDP
                     DKWEVASDRKQLAGILGYIADQVAKNNAKNLTLISKENEDAIRNDPNYKEND"
BASE COUNT         6167 a         4846 c         5436 g         6378 t
ORIGIN      
        1 tgagagttcg taatcccatc atcaacgttc gtttattttt gaatcctttg gtgcaaaatg
       61 gtgtgatgct gatgggctgc ctgggtgttt ttctttacgc catcgtcttt atcttaccga
      121 tcttcgtggg tcgggtcttt cactatgatg ccactcaaat aggttctttg tttattccag
      181 gttctatgct tacggcagtg atgatgcctt ttatcggtaa gcaaatggtc agcggcacga
      241 atccaaaatt tttgatcgcc ataggcttta tttcattgga agtctccttg tggatgatgt
      301 cgggactgtc gccgctctct tcgcggggag acattttaaa tattcttttc atccgtgggt
      361 tcggtatggc ttttcttttc gtgccgatca attcttcgat cttaggacaa tttaaagggg
      421 cggatctggg acaggtgtca gggctcttaa atcttttccg tcagttgggt ggcagtatcg
      481 gtgtggctat ggtggggacc cttctggtca agaacaccta tcaaaattat ggcgatcttt
      541 ctaacaatgt atctctctta aaccccgcga ctcaggccgc ttattatcct tcgttgaagg
      601 gcatggcgat gaagatgtcc gatgctgtcg gttttaatca aggagccgag gccgcgatta
      661 aaagtatttg gggccgagtg caaaaccagg ctttcatgat gagtttcttg cagctggtgt
      721 ttattctgat gttcgtggtc gccgtgggat ttattccgct ctacaggatc aagctgaaat
      781 caggcccgac caagattatg gacgcgcatt aggtacatta tttatccagt ttatcgaaaa
      841 catggccgcc agtttctttg ccctcttcat ggctttcctc cgcctggcgt gcgacttttc
      901 cggtgatgcc gacccattca gggtaaaaga tcgctaaaat cgcgtagaca aggatcgaga
      961 taaaagaaat ggcaatggct ccaataatga tcaagacttg cggttccata agttttcctt
     1021 tctcttccaa tgtcagtaat aggaataggc tgaatctaaa gtaggggacc cttaaaatca
     1081 agtcagggag ccccgactta cttcataaac tttacttgaa actttttgcc cttgatgcgc
     1141 ccttggcgca gacgctcaaa agcgatatcc gcaacagagg ccgtgatcgc cacataagaa
     1201 gagcggtctt ggatctcgat tttaccgacc ttgtcggcac tgaggcctcc ggcttcacca
     1261 gtgagggctc ctaaaatatc gccgggtctt agtttgtctt tacggccgcc tgaaatagaa
     1321 atagttttca tcgcagattc acgcagatct ttggcaagtc cgtgttggtt tttaaatccc
     1381 aaaattcgtc tttcaaattt cttgccggtg aacttttcaa tttccacgac tcgcaaagct
     1441 tcacgtgaag tggccaggga gaccgcgact ccactgcgtc ccgcgcgtcc ggtgcggcct
     1501 atgcgatgca cataaatgtc cggttgcaga gggaggtcaa agttgatgac cagttccagg
     1561 ttttcaatat ccaatccgcg agcggcgacg tcggtggcaa ttaaaatgcg gtggctccca
     1621 ttgcggaaca tggccatcac ccgatcgcgt tctcgttgat ccagatcgcc gtgcagacaa
     1681 gccgagctga tatcttgttc cgccaaaatt ttggcgagct cattcacatc cactttcata
     1741 ttacaaaaca caatggtgga gttcgaagga tgctgctgca gcacacgcat taaagtcgaa
     1801 attttatctt cgggctcggc ggcgtaaaca aactgctcaa tctgcggggc gctttcttgg
     1861 gtattttcaa tcgtgacctt gatcggattt ttttgatgct tattactcag catctgtatg
     1921 gtctcgggaa aagtggccga gaaaaaagcg gtctgacggg ttctgggaag ttctttgaga
     1981 atcgctttga tctcatcctc aaaacccatt tcaaacattt tatccgcttc atccagcacc
     2041 agagttttga ttttgctgag gtccatgcga ccgcgctgga tcaagtccaa gacgcggccg
     2101 ggagtgccca cagcaatatg caggccgctt tccagagccg ttatttgtgg acgacttggt
     2161 actcccccgg tgatggccag cacctgcaaa ccatcgtggc gacgacccaa cttgcgagtt
     2221 tccgtgacca cctgagaggc caactcacgg gctgggcaga ggatgagggc ttgaaggctg
     2281 cgctcttgca aatcgatttt gttaagaatg ggcagagcga aagccgctgt ttttccgctg
     2341 ccggttttgg cctggccaac cagatctttg ccagccaata aatgaggaat actttgcgct
     2401 tgaatcgggg tgagcttttc atagccgagt tcttgaacca cagtcagcag atccgcagac
     2461 aggggcaaag aagaaaaatc taatgataaa tctgttgagt tcaaaaagga cctcgattca
     2521 atggttcgcg cgcattttac cagaaaccct tctccagcgc caaagaaatg gtttcacata
     2581 ggaaaaaaga tgtggctggt atgaacgaaa tactttccag aaagcggagg atttttttct
     2641 atagctattc tcagatgact ccaaggaagg tcaccatttt gcaaacaaaa aaaagcggcc
     2701 ctgaaagccg cttttaagga tgagagactc cctttgataa aggaggatcc catgaagaaa
     2761 tatcccaaca cgatatctct tcggctatca ggaaaaaatc tgcaggtctt attcttaatt
     2821 ttgttattgc tgctgggcag ttgcagttag gtttttagat aggttctgtt aagggcttgg
     2881 tactaccaaa gtcctggtaa gtgacccaag gcgaattcgt aaatctgatc cggagaaagt
     2941 cctaaatcat ggcgctgcag ccactccgga tgaacgatgg catagtgagt cccggcaaaa
     3001 ttcattggcg atggtttcat atttaaggct ttgccgacca attttgcaca ataggtttta
     3061 ttgttattcc actcaaaatt aaagtcgaag gctctgccca gctctgcttc tagagcgtct
     3121 tcattaatca aaccatccgc aattttaatg cgaagatcgc cctgcttttt gacttgagcc
     3181 atgggttcag agcggcctcc aaaagtgggg tcggcttcaa aaagatgacc ctggtactct
     3241 aaggccacat gaaagagagc ctggggcacg cgggggttgt gatagaaatg caagaccgcc
     3301 gcccaagagg cacaactcca actaagccca aatgtgagtg caaaaataag tctcatgggc
     3361 aatcctctct ttaagcttct gtatccacaa tccatgtatc agtgtccgtg aagaggtgtt
     3421 ccagcaggcc gtccttgatg agatgatcat tcttgaaaag ctcatacaag ctcaaagctc
     3481 tgtccgtgag aaatttgtag gattccaatt tactgagata ttcctcttta gtcatttctg
     3541 gaggagccaa aggcttagcc acttgttttt gcccaaacaa cagagaatcg tagctgtaac
     3601 tattaaagac caaggcctct agcgctgtgt agggggagcc cgtgccaaag ttgcccgcgc
     3661 tcaggggcgg acgctgattg aaaagatcaa tgatcatctg agcattgccg atatccgtgt
     3721 gctcgttgca gtatttccaa aatggagtct cgagttgttt gttaaattta taatgaatac
     3781 ccaggaacca gcggaaagag tcccagttcg cggcgatttc tttgttgatg cctgcaatcg
     3841 agctgtagtc gcttgtggaa ttcggcatga gtcggcataa agtcataatg cttaacatgg
     3901 cggtttggat ggccgtcgac tctaaaggct caagaaatcc gtaagcattg ccaatcgaaa
     3961 agacgttttt gttccaagcc tgggcgtggc ggccggttct aaaatttacg actttatatt
     4021 tttcaaaagg accgaatttg gctcgggctt cagcgacggc ggtggcctca tcacaatatt
     4081 ttgaagagtg cacatagcca taatggtttt cttccctcat gggaatattc caacaccaac
     4141 cattgttcat ggtaatgact gaagtgtagc aattgatggt gttggcgtga ggtatttcaa
     4201 aggtgatggc cttgtcggtg atcagagtgt cggtaaaggg aataaactcg gtgcccagcg
     4261 ctttgccgag aaggcgggaa ttaaaaccgg aacaatcaat atagagatca taggaaagac
     4321 tatggccttc cacagtttgt aaggaagaaa caaactgctt ttcatcgaaa ttcacctggt
     4381 tcacttctcc atcgatgatt tcgatatctc tttcaagaag ctgggcatgt aaaaactgaa
     4441 tcaatttttt gttgtcgata tgataagaga aaggaatgct tcccaagaaa gaagtgatac
     4501 tgccatcgtc gttgcggatc acggggatct tattttcatt tattaaaaca gaaggccagc
     4561 ttgaattcgc cgtggagttt tcataaaagt aagattcata ttgatgactg gcaaaaaaat
     4621 taaaattgaa tttataatcg ccggggcagc cccaatcaaa cttgatccct agcttgagag
     4681 tcggttccac tttttggaaa aactcaagag ggtcgatctc cagagttcta tgtaaaaaag
     4741 ccacgatttc cgtggtggtc gattcgccga cgccaatcac tgggatctta ctggattcaa
     4801 taactgttgt ttttatttgc ggatgaagtt tttgcagggc caaggcggtt aaataaccag
     4861 cggttccgcc tcccaaaata ccaatggttt tgatttgggt ttttggacac ctgggaaagt
     4921 gggtgtagct gaggtcaaag gcattttcga tatgatcaat aagggtggcc atggtctatc
     4981 catcacaggt ttgaagattt tccgccaatg tgctattgaa gttttccaat ggcaagattt
     5041 tgtcattgga ttagaacaaa ttcactttca atttattttc taggagcgcc atgagcgaca
     5101 tcattgaaaa atccattacc gttaaagcca gcgtggatga aatctggaga tccttgactg
     5161 attctgatga gcttgaaaac tggtggggtg acggaatcgc gcttgaaccc cgggtgggag
     5221 ggtctttccg cgagccttgg gaggacgatg acggcaaaat tcaactggcc agcggcaaag
     5281 tcaccggcgt taaagtaaaa aaagaaattt cctttacgtg gcgagaaaaa gattggccta
     5341 aagaggccct tactcaatgc acttttgcta ttgaggataa aggcaaagtc cgagttctga
     5401 cggtaaaaca tgcgggctgg gagtgtttcc ctctggaggc aagaacgaag ctcttcaagg
     5461 atttccaggt gggctggggt tatcatatga aggaattgaa ggcctatttg gatgatgaag
     5521 gctagggaga ctcaatgcaa cgcatagcgt ccataaaggt cttttattca aaaaggcttg
     5581 gttcttatga aagtttcttc cctagttgaa aaaatgtgtt aatactattt tcagaaattt
     5641 ttttataaag aggttatttt catgagtaca ggtactgtta agttttttaa tgctagcaag
     5701 ggtttcggtt tcatcactcc agataacgga gctgacttgt ttttccatat ttctgaagtt
     5761 caaggtcaag agccacgcga tggcgataaa gttgaatttg aagttggaca aggcaaaaaa
     5821 ggtccttgcg ctgttaacgt aaaagttaaa ggcaactaat cttaaggttt aacctaaagg
     5881 cgatcttcgg atcgccttta gatttttagc tctctttcct tctcgcttct tcatttttta
     5941 tgacaagatc accgatttca tcctccattt atcggtccgc tctctctcga aaaagaaaaa
     6001 caaaagatcc ctgtccaatt tgttatttaa atcgctccct ttgtatttgt gattcgattc
     6061 cccagcttga tcttcaaacc aagctttgca ttgtggttca tcaccgtgag ctcaagcgga
     6121 cgaccaatac cggccgtttg gctttaaaag cgttgataaa tagcgaaatg cgggtgcgag
     6181 gggagagcag ggaggctttg gacctgagcg atttgttgac tcctgagtac cgcagtcttt
     6241 tattttatcc tgccgacgat gctgtagact taacccccga atttgtggcc gaagatccta
     6301 ggccgattca attgattgtg cctgacggaa attggcgtca ggcaagtaaa gttcattatc
     6361 gtcatcacga gctcaaagat ctgccaagag ttatgatcaa ggcacggaat ctggcaaaac
     6421 ttcatatgcg ggaagaacac atgagcgagg gcatggccac gcttgaagcc attgcctgtg
     6481 ccctgggcgt gattgaaggg gaggacgtca aggacacctt aatgaaactt tacaatacta
     6541 aattaaccgc aacgctgcag gggcggggag tgcctatagg cgaaaacgac ttttaatttt
     6601 tgcgatattt tccgggtgcg agcggacctt caagcggacg cccttgccgt cataggattc
     6661 cgaaagcaca cgcattttac tgcgaatttc gccaatggct ccctggacat cgtagggaat
     6721 aagaatctct tcatccacca tgtccgtttc aaaataactg atcaattgca ggcgtaaagc
     6781 cgccacatct tcaggacttc tcgcggatag gaagaccgct tttggaaatt cagctttcaa
     6841 ggaagcaagc tcttctgcgg acaggcggtc gactttattt aagaccaaaa gactttgaat
     6901 ttccccggcg ccgacttcgt taagcaccga acgagtcact tccaattgcg agcgaaaagt
     6961 cggatccgaa gagtccacaa cgtacagtaa taatgaagcg ctgagagcct catccagagt
     7021 ggacttaaaa gaagccacca gatcatgggg aagtttttta ataaagccca cggtgtcgga
     7081 aattaaaatt ttagggacag actccgggta aagcaaacga acggtggtat ccagcgtggc
     7141 aaagagttta tcagcgacta ggacttcgct tcccgttagg gctcgcatca aggaagattt
     7201 tccggcattg gtatagccga cgagagccac cgagatttct tggccgcggc gggcacggcg
     7261 agtgtcgtgc tcttgcgcga tggagttcaa ctcttcttta agttctttaa tccgatcacg
     7321 gattttacgg cgatccagtt ctacgctggt ctctccggca cccttgcctc cgacgccacc
     7381 gccgccgcga tcttcgcctc cgccggtttc acgcaatcgt ggtgctacat aagtcagcct
     7441 tgcgatttcc acttgcagtc gagccgcgcg ggttctggcg tggcggctga aaatttcaat
     7501 aatcacgcca gtgcgatcga ggacctggac gccggtcgca ctttcgagat ttcttaattg
     7561 agaaggggaa agatcgcagt caaagataac aatttgagcc cgctctttag gagctttatg
     7621 gggagtaaag tcttcgtttt ctgggtcgac ttcgtcgtcg tctttttcca tttctgaagt
     7681 ttgatttttt tcaaatttga gagcggcttt atgttttttc tttaccacca gggggccaat
     7741 gacgcccgtg ccgccggtcc actccgccag ctctcttaat tttccatcac cgaggacgct
     7801 ggggctgcgt tccgaaggcc tgcgctgaga agtctgtccg ataacatcat agcctaaggt
     7861 ggttaccagg cgcgcgagtt cggcaagtga gccttgagtt tcttgatcgg aagtttttgg
     7921 aagttgaatc cccacaagga gagctttgag gggggcggcg tgagtaggtg agttcatagc
     7981 tcaacttagc tctctccgct gtaagaagca agctttcccc tgatcgtatg ttttatggga
     8041 tctgaataat ttgatcagcc cataattgat ggcaaaagtt ggcataaggc aatacttcaa
     8101 caccctcaaa aagtcggggg agtcgttcat tgcagacaac aatttttttg gaaattttaa
     8161 agtcttcttc aaaagctttt aagcccttaa ggtccgtctt gtctatatgc tgggaaccct
     8221 ttacttctat acagatgagc ttttttgatt ttgtcttaat gataaaatcc acttccattt
     8281 gtgaagtgga tctccagtaa tataactcaa aggccttttc tgagtagtct tgataggctt
     8341 tcaattcagt aaagataaag tgctccaagg ccttgccata ctctggagtt cctacgctca
     8401 gtttttcacg acctgtaaga taattactta ttcccacatc gaaaaaatag aatttggggg
     8461 ttattacagc ttttcgactt ttggtttcct tgaagctttc cagcaaaaag ccagttaagg
     8521 tatcttgtaa gatttgatag taggcactca ctgtcctcgg tgaaagttgg acatcgcgag
     8581 cgattcccgc ataatcaatt tgttcggtat ttgaaagggc cgcgacatca agaaatttgg
     8641 agaaatcacc caaattccga acatagcctt cggctttgat ttcttcctgt aaataaattc
     8701 ccacgtatgc ttttaaaagt cgctgcggtg atttagtggt taaaacttga ggcaagcccc
     8761 ctgcttctat aagtctatgc agattttgag gttgtgaggt ggctgaggca gataaatact
     8821 caacggaggt gatcggatgc aaagagaggg gataagcccg gcctcccaag agattttttc
     8881 cttgattgcg aatctttcga gcgctgctgc cagttaaaat aaaacggagt ttcttgttac
     8941 tttcaattaa agaatgaaca acatccaata gttcaggaat tttttgaatc tcatcaataa
     9001 tcacaatatt attttttgaa ccggagataa tttctcttaa gaaattaggt gatttctgaa
     9061 gatctcggaa aaactccgga tccagaaggt ccaccagcaa tgcttgcgga aattgagacc
     9121 taattaaggt ggatttcccc gtctgtcgtg gtcccaaaag tagaaggctt aggtcgttga
     9181 gataagattt taggtttaga gttcgttgat acattatgta tagtatatgt atcattttaa
     9241 tgaaaaaaca acatatatac gtcgtttttt cattaaaata gtgcatctag aaacgtagag
     9301 ggggaattta gggctccttc atagaaaaaa gcccgtggag tcccaagact ctgcgcccga
     9361 gccagcagat gataaaagtt ggaaggaaag cttcaaaacc aaaaaattcc caagccatca
     9421 cggctccggt ccagggaata tgagaggccg aggcaaagac tccgaccaat cctaaagagg
     9481 cggcatagag gctcggtaaa gccacccagt gtgaaatggt aacgccaaag gtcgcgccga
     9541 tcgccatcag cggagtgacc tcgcctcctt taagaccgga ggcggaagat aaaatggtga
     9601 agagactttt ccaggcaaaa tcaaaagtct ggactccaga ggtgagtgcg gcatcaatta
     9661 agggcacgcc gagaccattg taacgagttt ctttcatcat cagagtgaga gccgccaccg
     9721 caagaccacc cacaaacggg cgaagccatg gccatgatat gcgcccgaaa atagttttct
     9781 cagagtactg catgagaacc acaaaaactt tggcgagcac gccgaaggcg attcctaagc
     9841 agctccattt tgctataaga aggaggttcc aaggaagcgc tgggaacaca ggatagacct
     9901 tatgatgagc gccccaccac agggcgatcc aatgggcgcc aaaagaggag atccagcaca
     9961 ggggaagata gcgcagggac cagcgccgcg acggcgtagc ttcaagagca aaaagagtcc
    10021 cggcccaggg aactccaaag accgcgccaa aaccagcggc gagaccagag cggataaagg
    10081 cttggcgaga aaaacgaaga ggttttaatt ttttgtcaaa aatttcccgc cagaaatccc
    10141 ccagagccgc gccaaattgt actgccgagc tttctcgacc ggtggaggcg ccgacaagtt
    10201 ggcttaccac cgtaaagaaa aaaattaaaa gcgctgttaa aaaaggaatc agggcatgtt
    10261 gatcctcaac gccgtgaaga atctttttag tggtggggag tttttttcca aaaagccaat
    10321 aggtaaaacc tgtgactatt cccaaaaggg gaagcagtaa aaccaaatgg tcatggctca
    10381 aaaaaaactg tgaagtctgt tccaagagga ataaaaacaa ggctgcaagg cttccaaccg
    10441 acataatcac aaaaataaag cataaaaggc cgaacagaat tgcagacata ggttcttttg
    10501 ttgtagcctt tagccaacaa actgcaagga tttttctatg tttaaaggtc ttattggtta
    10561 ttttacgggt tccattgtcg caacaattct ggggatgatt cttgcggtct tcgtaggcta
    10621 ttattattct ggtactactg caggagccct gcaggcactt tttatcgctt ttattttagc
    10681 ggttttggaa gtctcccttt cgtttgataa tgcgattgtc aatgccttga ttttaaagga
    10741 gatgactccg gtttggcgac agcgctttat tacttggggg atgcttgttg cagtctttgg
    10801 aatgcgcttg atctttcctt tagcgattgt gtctgtcgtg gctaaaattg acccgtggtc
    10861 cgctcttcta atggcgacct ttaaacctga tcaatacgca cagttgatgt tgtcggctca
    10921 cctgggtgtc tcagcttttg gcgggagctt cttattgatg gtcacccttc gttatttcta
    10981 tgatgagacc aaagatgttc attggattgc catgatcgaa agacccttgt cagccctggg
    11041 aaaagtggaa gccatagaga ttggcctgac tctgttgaca ctgatggggc tttcgtcatt
    11101 cttccctgaa aataaagatc agctcacttt tttaatagcg gggatagcag gcctgatgac
    11161 ctatattgta gtcgatggca ttggcggctg gctcgaactg tctcaggagg gtatgaagga
    11221 tttgcaaaaa gcaagcgcag gcatgttcgt ttatcttgaa gtcctcgatg cttcctttag
    11281 cttcgacggt gtggtaggag cctttgccat cactcacaat ctttttatta ttatgatcgg
    11341 cctgagtata ggcgctttct tcgtgcgctc tttgacgatt ctttttgttg aaaaagaaac
    11401 tttaaataag tttttatatc tggagcatgg ggctttttat gctatcggca gtttggccat
    11461 gattatgctc ttggatcctt ttctgcatat tccagagtgg gttacgggat tgctcggggg
    11521 ctttgtgata ttgattgctc tttactggtc tattcgcttt cccgaggctc cttccagagt
    11581 gcggcatcaa ggctcctcag aaccttaaag cctcggaaga gattcataaa gggctaatcg
    11641 aaagcttccg accagacacg ggttttatca aaaccatcga cttcaagaag ttgatgttta
    11701 acttctttaa tcatgccgcc attgccacaa agatagaaag tggtaggggt gctttgatag
    11761 ttgaactctt taagaaagtg ctgaacatag ccttttttcc ctttccaatt ttcctgggga
    11821 cgactcaata caaattcata tttgaagttg gggaattccg cagctaaggc atcgagttcc
    11881 tctttcaagt aaatatcttt ttcgcttcgc acgccaaaga gcaagcgata ctgtaaatga
    11941 gggaaaagat ctttcttgga aaccaagtaa gacaagtgtt gggaaatgcc ggtgccagtg
    12001 tttaagaaaa caatttgctc cgtgggaggt tcttgaaaaa aaacttttcc gaaaggaccg
    12061 gtgaatttta atgtctcatc cccctttaaa gaccatatgt aagtcgaggc caggcctgtt
    12121 tcaacaaatt taaaaagcaa acgaaagcct gttttttctc gttcatcgga agcaatggag
    12181 taagcgcgaa gtgccggctt ggcatcagga accgggacgt ggagcatgac aaattggccc
    12241 gcccgatatt gaaaagtttc aggactttcg gtttttaaaa tcaattctcg gacagtcgga
    12301 gtgtgatcta tgattttttc aattctcatt gaatagacgg ttcttgctgt agacattcgt
    12361 aacctcaaaa tagagccata aactaagaat agggagcatt ttgaataatt caaaaagtct
    12421 aggcagtcca atcatttaac ttgtgacacc gcacttcatt caataaaata aaaacgtgaa
    12481 tttaatgaaa cctttaaaag tatgcggacc acatactcga aatattggca aatggaaatc
    12541 ttcgcttgta tttcttattt gttgggcgtc cttttcctat aacgtccgag ctaatgaaga
    12601 ggctctgaat acagaggctc cctcttcggc tcccgcttct tctccgactc cgactcccgc
    12661 ttcgacggga gagaaaaaaa atccgcggct gattttaaaa acagctagaa acgatcagta
    12721 taccaagcca ctttacttcg aatccatgga taatttattg cctaatgtgg aattcgaata
    12781 tgatttggcg acgggagagg gaaagtcctt acgtgttggg gatacggtta ttgatgaaaa
    12841 gagtttcttt tttgcgatgg tccctgtctc caaatttcat gctaaaatga gttctctttt
    12901 ggatgccaaa gaggttgata aaacggcctt ggtcatgggc tggcccgagg ctttgcaatc
    12961 gggtggaact cttgaaatga tcgcgcatag tggaaaagtg gtgtggaaag aggagattac
    13021 cgaacaaaaa cgtgaccttt ggaaggataa gttggaattt tggaaaaaag gtcttgaagg
    13081 gaagggcgtg aaaacagcga tcctttcgaa ggggagtgtt ttctccactc agttcggcat
    13141 cctcgatata aaatctcaag gtatgcggcc ttatagtgag tccttccgtt tttgtctgac
    13201 tcaaactcaa gggcgggagc aatctcggtt atgttcccaa cgctatatta ctcgcctcag
    13261 gggaaatcag ctcatcctga aaaaatcgaa gagcatggtt caaccgcggg tcttattgca
    13321 atctcaagct gcggagctca aacaaagtct ccccgtaccc cgcgatacgc caacgtcttt
    13381 ctttgctgag cttgcaagtg gcgagactta tgaatttatt gcaacgccca ataaactcaa
    13441 tctgatggat gtgacagaca ccaaaactcc ggaaaaaatt cgcatcatgg cttggggcgc
    13501 ccggcccacg acaccctcaa cgattattaa tccagattca tattcttcag tgactcgtgc
    13561 ccttggcttt gaagcgacca ttggagataa acgtaaattc tgggaagcac tcattagctc
    13621 tgaagatccc aaaatttatt tgccaggtca ggggggcgga ctttttactc agcgttttga
    13681 attggctgaa actcctcgcg ccaatgcgag accttatttg gaattgcaca ctcctaccgg
    13741 aacttaccag gatggagtta agcttttcgg taaaaaaatg cccgatgtaa aaatcacaag
    13801 ttctgaaaac tcgattgaag ttgatgataa tgatcccagc gctttcttgt ggagatttaa
    13861 agctaaagag tccggtgaaa tcaatcgcag ctatttgaat ttggagcatc agggaaaaga
    13921 atataaaacg ttttatgagc tctataaagg attcccccgt gaaattagtt tgcgtttgtc
    13981 gggcttgatt caagcgagcg ggcaaagtgt tttcatgggc gagaccgctt ataatcaatg
    14041 gtttgaaaat cttttgggat ggacgaatta ttgggtcggt cgccaacgtt ggggtataag
    14101 cgccaagtat tttaaaagtt tgaccaagct cacggtgaat gccgcgaaag ggacaacggc
    14161 ggacttgacg gtcatgaatg tcgatttgaa atatcgctta gtgcctggtc tgtggggacg
    14221 ggatgaaacg ttgggagcca tgttggacta tcaaaaaata gattttgata agatttccgc
    14281 accgatgttg ggggtggggg ctttctgggc tcgatcgatg ccaaaagttt ttgatgactt
    14341 ttttaatttt ttaccgctga tgcagtatcc gaaatgggtg gacatggagt ttatctatta
    14401 tccattatcc atgtcatcgc gggtgactct ggagtcgaac ttcgccttga atttccatgg
    14461 taaagtgcta tggacgaaga attggtttgg cgaagctggt ttcggtatca aacgctatgc
    14521 aatagtagat gctcaattgc ggcaaaaagc ggctctggat accttttacg gaacagtagg
    14581 cgtgggatta aatttctaaa aagacttcta aaaataagtg gcgattccgc cccggagcac
    14641 cagcccctga agggtatcgc tcgaagcccc ggcagtggtc ttgatgtagg aatagcccat
    14701 atcaaagcgc aaagccgtcg aatccgttat gccaaaccat ttaaaaaagc cgccgccaaa
    14761 gaactccagg gtggaacccc catcccctgt tttcggagtc aaggagccaa caatgccttc
    14821 gctggtgatg ccaaaaatct tagtttcccc cagacgattt gcagtcaggt tgtaatcggc
    14881 aaaggctccg ccggaaaatg aagagtaatg gcgatcggta taatcggagt cgatattctt
    14941 gtagccgaaa ataggaccga attcgaagga ttccttattc cagccgaagc ggataaagac
    15001 atttatatca ctgtttttca aagtcgtatt aagagaagga acagtataag aagtagagtt
    15061 aaatccaaga ctgcctccaa taatatactg acgttttcct ccggcggtct cttcgggtgg
    15121 ttccgtcttg ttgatgctca aagaataggt ttttccaggg atcagacttc ctgaaaattc
    15181 gacaaccgcc cggttgcctt tgattttttc aacgtggatt tgctgttgag cctgagcgtt
    15241 tattgatatt acaagtgatg ctaagaaagt aaacttaatc acgcgaacct tctgtttctt
    15301 cctgaaagac atggtcgggg ttggattgta tgtatctata agctatctga ctttttctaa
    15361 tattcaaaac gaagaaacag gcctccctgt aaataaagtg tttcaatctg aaacatttat
    15421 atccataatc ctcactttta agaaaagccc ctcttaaatc cttaagaatt tctggttttg
    15481 agtcgaatag aagaggtatg gaggaactcc tatgtcttcg cagtcagctt caagctgggg
    15541 tgatattccg agttgggctt atgatgctgc acaggcttta atgcaatctc tcaaggttgt
    15601 ggatcctgtc acctatgctc attgtttgcg ggtaggggaa atgtcccgga aactggcacg
    15661 cgatgcgagc ctgaatgaat atgaacaaaa acttgcggag ttcgcaggga tttttcatga
    15721 tattggtaag attggcgtag accaagaaat tatcggcaag cctggaaaac tggaacctcg
    15781 tgaaatggat attatgaaaa accatccttg catgagtgaa caaatcgtac gtcctctggc
    15841 gacgcacagt ttcttcaggg aacttttgag ccctattcgc agtcatcatg aacggatgga
    15901 tggtgaaggt tatccagata gacttgtggg tgataaaatt tcagtccttg cacgagtaat
    15961 tctagttgtt gatacctatg atgctatgtc gcagacccga gcctatcgca aaggtcttcc
    16021 ggatgagatt gtgtatgctg aacttaaacg ctgttccggt acgcagtttg atcctcaact
    16081 cgtgaggatc tttctacaag cccacaaaac ttggaatgct cttgagacgg aaaaagagag
    16141 ttttcagcaa tttgttaaaa aactgggtta aagctctcca gagttctctt acattttatt
    16201 tgtgctttca aagattttat ccgcggaagc cgcgataaat ccctgataga gcttgccgtt
    16261 gggcatggga taacgttttg caaactcata atagcaacca ggaatggaga aagtgccatc
    16321 gctgaacttc actgggattt cgctcgccat agttgagctt tgttccaaga attccgcggg
    16381 agttcctttg acttctcctc ctgatttgtt taatgcgaag cccttcaatg ccaggaattt
    16441 attgagggcg gaaatatcat tatatttttt gagtgcattg atgaagactg taaagtgatt
    16501 cggcctaaaa ccatgggcag ccacccaaga agcgtattcg ctttctttgg cgagctcgtt
    16561 ataaagttta taggagaggt cccagggacg tccacagaga gcaaaagtct ctgattgaat
    16621 caccgagtca ggtactgagt ttgttaaggt gttgatagtg tcacgcagga aaggagaggt
    16681 tttttccaat tgcagttcac tgatgaaaat tttgggcaaa ctttcatccg gatgttgcca
    16741 atgtctcgcg tacaatttct tgtccacaaa atggtaatca tttgcagtca tcacataacc
    16801 cagcttttca atttggcgag ccaaggattt gattccgagg cgagggtgat taaaggtacg
    16861 aagagctata tgatcgttca ccacagtttc gccttccgca gtcaaaagat catagatctt
    16921 tttagccgcc gggttcattt cacaataatc tttccacatt atatcgagaa gtttgtgaat
    16981 tggcatttga gcgttcatga ggagatcctc ctttagggga taattgtcag cgttccctga
    17041 ctctataggc ttcaatttca gagagcaaaa aataaccttt agcgttaaca gttggtgata
    17101 aagagaaaac acaatttaat ctgcctatgc tgttagaaat ccaacaagtg gaaaaagtga
    17161 ttcatgaatt aggccctctg gtgcgctccg aagttttggc ttatagccga acgcattctc
    17221 tgaagttgcc catttataaa ctcagcttcg gcagcgaaga tccccaggcg ccagtggtcg
    17281 gtctggtggg tggggtgcat ggcttagaga gaattggctc ccaggtttgt gtttctcttt
    17341 tgcattcttt cagtgaactt ttgctctggg atgaagtcat caaagagtct ctaaagcatc
    17401 tacgggtgtt ttttatcccc gtggtgaatc ccattggcat tttaaaaaag acccggagca
    17461 acccccgggg agtggattta atgcgaaatg ctccgatcga aggagagcat ccgcctcgat
    17521 ttttgggtgg acatagatat tcttcaaaaa ttccttggta tcgaggagtg gaaggcgcac
    17581 cgatggaggt ggagtctcgg gctctggtag aggcagtgca gaaggagatt tttcaatcgc
    17641 ctttggctat caccgtcgat tttcactcag gattcggaat tcaagatcag atctggttcc
    17701 cctatgccaa gaccacaaaa gcttatcctg aaattggtct agcacacgcc tttaaaagtc
    17761 ttttcgatcg cacctttcct catcattttt ataagatcga acctcaagct ttgaattata
    17821 ccacgcatgg cgatgtctgg gactatctct acgataaata tagggagcaa tacgctttaa
    17881 atggttctgc caaaggtgtt tacctgccct tatgtttgga gatgggatcc tggatgtggg
    17941 tgaagaaaaa tcctcttcag ctgttcaatc ttcaaggtca ttacaatccc ataaaatctc
    18001 atcgcatgaa acgaactttg cgtcgccaca ataccttttt cgagtttacc ctgcgtgcgg
    18061 cgctttcgcc tcaatcctgg gcttatctct cagaagagca acgagtgaag catgaaactc
    18121 ttgccacaga gctttggtat gcttgaatgc caaattaagg tttctccgtt ccgcagcgtg
    18181 ttacataaga ttcaattcga gtatgcaaaa ctcccaaaac agtaagagac attgactgaa
    18241 tgaaggaaaa gcgatttcct tttcgaactg tgaagatatc taaaactaca gcccgtcggg
    18301 gaaacttaaa acgaaggttt ctatggctaa cagtttattt gttagaaatg atgacctttg
    18361 ctgaaaagtc tgtagcggac cttcgcctac ggacggagcc ttcttcgtcg tcgatctcgt
    18421 ttgtttctga agtccttaaa gaagaacgaa cccagcaccg ggtttatggt caacttcgta
    18481 ttgaaggcat gaaatatctc acggaaattt ctgaaactcc tcaattaact tacagtcaat
    18541 ttctgtcggg ccagttgagc tatgtgggcg aaaccgattg gctagagaac gcagtggatg
    18601 tggctgctgg gacgtttttt tctcaaaacc aatctcattt tgtagtgcac gaactttata
    18661 cttcgccacg gacgcaaggc taccgggttt atgtaggtcg caagaaaaat ggctggagcg
    18721 aagctgatca cgaatggaat ctcggagtgt ggcaacccta ctttgagttg gatgcgcttc
    18781 gtcctcagga gcagggatta accggacttt ttctcgatgt gaacaacacg gagtggcaag
    18841 ttttgggaat ggtcacgccc attttcattc ccaccctggg tcctgatatt cgcgaagagg
    18901 acgggggcct ggtggctgac agtcgttggt accatgctcc cagtcgtcag gcggatttga
    18961 tcaccaataa gccaacgccc atcacttata aattgagtat tccagaagcc gccaaacttg
    19021 cggcccatgg gggctatgcg tttatgggaa gagtcggtaa caaggacaaa ggattctggg
    19081 ccgttagcag ttatggctac atgccagtga acaagctttt gctaaaacga aatataaaat
    19141 accaaatcaa tcaagatgtc ggtgtgattg tatctccgga tttgaactat cactcggtct
    19201 tctctacgga tctgggttat acgcaaggca acgcacgtgt gacgttttcg tttttgcaag
    19261 acaatcccga agacaagctg ccggatcagg attgggtcct gcaaaaaatt tatccactcc
    19321 gtgcttatac cgcgggaatt gaatggacaa ttccgaaatt tttctcgcgt tccatttccc
    19381 tgcaactcga ttatttgcga gtcgatggag gggagatcga ggatatcggc agcgatggtg
    19441 cggtggatga tctgaatctc tttgatcagc gtgtgaagtt tacagacgcc gtgaaagtga
    19501 aaatgcaagg ggagttggcg cgaatatatc gtcaccctct cagggttcat ttttcctacc
    19561 tctatgacaa agagcaaaag ggatcgatgg tgaatacaga gtttatgttt tacccgaaac
    19621 aagagttggc tcttttgtta ggggcggact ttttaggaac ggcagagaat acgcctaaga
    19681 cttcagggtt tttgaatcaa tatcgtgcga atgatcgtat ctatgggggc atgacctatg
    19741 ttttttaaaa gaaatctctg ctatctttta agtgcgattt taatgatgac ttcttgtacc
    19801 cctaaagtgg gtgaggctcc tccgcaagtg gagcaacaaa aactgaataa gactcagtgt
    19861 ttgtcaggtc ttcagtcggt ggttgagtct ttcttcgctg gagaggccac ggatgcagcg
    19921 gtgacttccg gttgggattg cgcttcgaca gccatcaaga cttttaaaaa atatgtttat
    19981 ggccgcactg gcgaccgctt cgaagcgact gagttgtcgg actttttaaa gaaaaatttt
    20041 cttgaggcct caagtcccga aatcacgccg gctctccgcg aagaatttat gcgtattaag
    20101 caactttttc tgggggggaa tattaattat gtgactcgcg ctgagttgga taaaattctt
    20161 agcatcttgg gaaatctcca agacatctct ttgcgtttaa atccctatat gaagttgatc
    20221 acgcaaaagt ggacgattgc gggttcaact catctgcaaa atgatattct ttattttgaa
    20281 aaagccagcg atgaaattca agacgccgct cgagccctgg ccaacatcat ccttgaaaat
    20341 aaccaatcct acgagctgga tcattttgcc acttttttac gtgaattttc aaagttctct
    20401 cagcgtgctt ggcctcttgc agaggaaatt gaaagagaca tgcccgtcgt caagaaaata
    20461 aaaaaagcta tttccggagg ggatccagat tccatcggcc ctgcagattg gagaagtttt
    20521 gttctcctgg gtgctcgcgg ttacttgcaa tatcttcgtt attactactt tattaaatcc
    20581 gctgcggaaa tgggttctag catccgctta ggctatctgg cgcgttcttt ggaagatctc
    20641 ttgggtgcat ttcaagatct ggtcgagcag aaaccaaagg atcccgtctg tggaacgtcc
    20701 aactcttctt gcatttcaaa acaagaaatc gatgatatct tgaagacctt ctctgatatt
    20761 tggcaggact ttcacacttc tgacaaactc gtcgacgagg ttatgaaact caaaaagatc
    20821 tatttcggcg gcagtgatgg gaccatcacc agccgcgatt ttgaacgcgg aaaaaacaaa
    20881 gtcgggaaat taaagtttgt ggttgaaaag ttcctgcctt atttccaggt ttacagtctc
    20941 gagtgggacc gcgcgaatta cgatactgct tcagcggagc agtattttaa cgaagctcag
    21001 ttgagcttgc agggcagtgg ggaaagtttt ggggctcttc tagaggagtc ctacaatctt
    21061 gaaaatctgg cgagcctgct gagcgaagtg gctcgccttt atccgctcca ggatccaagt
    21121 aaagatccta acagtcatcc tgctttggtc atgaagaaaa tggtccctct ggttaaagac
    21181 atgaaaaaca tcattttttc tgacggcaat ggactggtta aaaagaatca gtggagcctt
    21241 tttcttaaat tctcagcgaa tttctacaat acttatttgt ttcattatta ctttttaaag
    21301 caagagacct atggcaccgc tccttttcta gaggccttcc aaaaaatcac cgataaggtt
    21361 ttgaatattg cgaaggatgt ggttcttctt aagaaaaatc aaactctccc gattgatgag
    21421 ataaaaactt tggcaaaaga tatgtcctct gtgggctata ttccaccagg tttagccccg
    21481 gattctattg atcaactggt caaagtagtt ttgaatcgtg ttctatggcc tgcagagctt
    21541 cgcttgaacg gtacgattcc taatggcata actccagtaa gtattgagaa tactcgtctc
    21601 gaaattaaaa tctggtatga gactgagaaa tatttatatt ccctcacagc ttcacctctc
    21661 aaccctgcgg acctgcaagc tcaagtagct aaaaagttga aggatgcaaa aatttctgca
    21721 gagttaagaa ctggcttgag tgaagtttcc ctgatgttga ccgatggtgt tccgcagccg
    21781 atcgattctg aaggccatct catcattacc aatattggga agctcacgta caatacagtc
    21841 agtatatcac gcttaaatat aaatcgtttc gctggtcgcg tctttattcg tgctgctaca
    21901 acggatttaa atcgcctgaa aagctatgag ggaatagatc ttaaagaggc tcaggaattt
    21961 tttgatctta tcaggcctgt ggttgtggaa ctcggccttt tggaagagaa aaacaccgtt
    22021 tttatgcaat ctcgtttccg tgaagccaat attttctctg cccatagtaa cggggatgcc
    22081 tatgttaatt ttcaagagat gacggacatg gtgggcatga ttctgtcagg gattgctgtg
    22141 aatgatatgt tccgtaaaga tatcatggcg gcatgtggtc cagtgaaaag ccaggatgaa
    22201 acctacgttc aagaatcttg tattcgtaaa atttatattg ataaaacagc tagctacatg
    22261 acagcgacgc cggagtatat caagtatttc aagcagcttc agcctgatga atattctggt
    22321 tttttagcga atattttaaa agccgcaggc catattccca acaatgacga gaacgtgaat
    22381 atgctagatg ctgatttagt gcctcatgtg ttccagtact tagaaatgac ccttgcgaag
    22441 tttgatgcca acaaaaatgg agtgattgac ttagatgaag ccaaggtcgc ctatccctcc
    22501 tttaaaggga ttttgatgga gctcactaaa gatcaaactc tcatcaaaga gaaagatctt
    22561 ttggccttgt ttacttacat tctgcactat ggaaaaccac ccgatggagt cagcgattat
    22621 ctctttaaat ggctgccgtg gaagtcagat ccagacaaat gggaagtcgc ctcagatcgt
    22681 aaacagcttg cgggcatcct aggttatata gcggatcaag tggcaaaaaa taatgccaag
    22741 aatttaaccc ttatttccaa agagaatgag gacgccatcc gtaacgatcc aaattacaaa
    22801 gaaaacgatt agagataaga ttaaatt
//
LOCUS       DATBTE010000033        46906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_413745, whole genome shotgun sequence.
ACCESSION   DATBTE010000033 DATBTE010000000
VERSION     DATBTE010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 46906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46906
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413745"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2012
                     /locus_tag="VIG33_01210"
     CDS             <1..2012
                     /locus_tag="VIG33_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269478.1"
                     /translation="NVMKQLNQTQLRNSMSCLLEITSESYCSALDASNLFKEQMSRFQ
                     IVSGKDDTLKITDSVDMQNSTKGPLQGYYILTQQIPVITSWLEKIQRGVEPKLKTDAK
                     FKNETIQTVMNFYTTENNLLGDINSSKTTLASQTSLQAKKNMVLKTLLSISDRLAGNI
                     ANDNETNFFTINMRPMEIPFALIGREVPPEVLGKGKSDFMQSPESWLQANMSNLSEFE
                     KPEQLIETIHSNLKSLIEASQASAVSYYNEWFIYDNAALYVDSVTGMRYNVRDSLQNT
                     NDYLSSLEEKIKSSNSADISIIPTIDETRFLISTVLEKYENIRLLGETYRAATTYENR
                     KATIEKGKQLHKELIESVYSSFSVLKGKSGFLANRMAIIVKNDFQTTLKSGADFSNEN
                     VKKIFYATGDAAFEKMRQMSSGNPKDIMMDLNNAQMTHKENLTAIEMMLKNHFLAMIS
                     YTKMVSQGISPSDEDVSRDSFKRAWSDYWEEYPIASRNILGVILEGIIKPFDFYISFY
                     KGNHDRYPKSRTTSLGNNKLAASVDNENEAAKHMYELYCIQSLAFTDWRPFQALCKDV
                     VLKSPFNTATLSPLAQQKFDTSLNVSYDYKLAENLTDQTRKNISKIREQLRYAAMDRN
                     AGKTYREKMSQVKLEMAKNHSARICALRDYGRNNFVMYLTLGIKK"
     gene            2032..3210
                     /locus_tag="VIG33_01215"
     CDS             2032..3210
                     /locus_tag="VIG33_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8269479.1"
                     /translation="MNDLRLHFKSFRQFWPFYVSQHTNPINRKLHVGGTLCGLILATF
                     LFSMGHWVLAITLGLVVGYGCAWLGHFYFEKNRPATFHFPIYSFMADFKLIYCLLTGR
                     SLVPAGAKKIIFITGAGRGIGFALVRHFLNQGHQVFAAVRSSPPALDELRKKHETLQI
                     VEMDVTKEDQLIQVANQLASTLSGNEAFILINNAGISGGGPFELMTSQHWQDMFNVNV
                     MGLVNVTRVFLPQVRRTKGRIINIGSMSGRVASPFVSSYSSSKYAVRAVNDSLRRELG
                     PLGVSVILIEPGPIATDIWSSSMTNSQKMMENASPEILKVYQKNIDKLEKEIRNIESS
                     TVSVEYLTPFVDEAVNSPHPKPYYRVGKNIGFIFILTHFLPASILDRILGGLRFEKMK
                     "
     gene            3307..4275
                     /gene="pfkA"
                     /locus_tag="VIG33_01220"
     CDS             3307..4275
                     /gene="pfkA"
                     /locus_tag="VIG33_01220"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEY8269480.1"
                     /translation="MKRIGVYTSGGDAPGMNAAVRAVVRMAIAKKLEVYAIYQGYNGM
                     IEGKMSPMQPRDVANIIQRGGTVLKTGRSSDFMKPEFRKKAADNLRALGIEGLVCIGG
                     DGSFRGAHALWEEQKIPVVGVPGTIDNDIFGSDNTIGFDTAVNTALDAIDKIRDTAAS
                     HDRLFIVEVMGRNSGHIASYVGLAGGAEDIFTPDANTTVDKAVDRIRDSIQRGKTSSI
                     IIAAEGQKPGRAYDLAEAIRKKSGLDAKVCILGHLQRGGSPTASDRVLASRMGAAAVE
                     ALIKGQSDIMIGTDGPHLVTVPLEQVVKSEKKTSMDLINLATALSL"
     gene            4354..5475
                     /locus_tag="VIG33_01225"
     CDS             4354..5475
                     /locus_tag="VIG33_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8269481.1"
                     /translation="MKKLIITLLAVLPLIAACTKKSTEILVGEFGSLTGNDATFGLST
                     NKGLRMAFEEINAAGGVNGKKIKLITIDDQGKSEEAASAVTRLITQDNVVAIIGEVAS
                     GRSKAAAPIAQKYQIPMISPASTNPSVTQVGDYIFRTCFIDPFQGFVVAKFATQNLKS
                     KKAALFRDVKSEYSVGLADVFAVEYKKMGGELVADLSYQGGDIDFKAQLTQIRSKNPD
                     VIFIPGYYNEVGLIAQQARQLGIKAQLLGGDGWDSSRLSEIGKDAVNGSYYSNHLTNE
                     STEPLVIEFVKKYKAKYNETPDVMAALGYDAAKILAEAMARTKELTSKNIRDEIAKTT
                     DFPGVTGKITINAERNAVKSAVIVQVQGAERKFITTITP"
     gene            5484..6386
                     /locus_tag="VIG33_01230"
     CDS             5484..6386
                     /locus_tag="VIG33_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8269482.1"
                     /translation="MQDFIQHLINGISLGSIYALIALGYTMVYGILQMINFAHADVYM
                     VGAFTAYYAAKWMGLENSPGISTLIILLFTAMFVCSLLGLAIERFAYRPLRRSPKLNV
                     LITAIGVSLFLEFAGQVVFGADPKVFPEVMKDFVISSFGGIDLKSSDVTVLGVSLVAM
                     VLLNFIVQKTKMGRAMRAVSTNAPVAAMLGVNTNRIISFTFIVGSSLAAIGSILVGMK
                     YPKIDPLMGMMIGMKAFVAAVLGGIGNIQGAVLGALIMGLSEEMVVAYLSSTYRDALA
                     FGILIVILIVKPTGLLGKYAVEKV"
     gene            6386..7345
                     /locus_tag="VIG33_01235"
     CDS             6386..7345
                     /locus_tag="VIG33_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY8269483.1"
                     /translation="MKTFKAPLLSIIGLLVIGGILDFGVNPYVQLMVLYAITNSIMAM
                     SLNLVNGYTGQFSLGHAGFMAIGAYVSAFFSTHFPFFAGPGIILTTLGGGLLAAFAGW
                     LVGLPSLRLKGDYLAIVTLGFGEIIRVALLNIDSLGGPRGISGIPSFPNFFISYSYAC
                     VWAVICFFVIWRLVKSSHGRGFLSVREDEIAAQAMGINTTTMKVRAFVLSSFFAGIAG
                     AIFSHVTNYISPSTFTFLLSVNAIIMVVLGGMGSMTGSIAAAIFITAMPELLRPLQDI
                     TGIDLRMVIYSLLLILAMILRPKGLFGEYEITDIWRKYVRKSA"
     gene            7326..8096
                     /locus_tag="VIG33_01240"
     CDS             7326..8096
                     /locus_tag="VIG33_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8269484.1"
                     /translation="MSANLLEVKAITMQFGGLKAVDSLSFNIPKGQLAGLIGPNGAGK
                     TTVFNMLTGVYQPTLGEILLNGQNLVGKKPYQISHLGMARTFQNIRLFKNLTVLENVL
                     IATHQHITYGLLDSVLLTQKYQKQESEMYEKTFNLLKIFHLEPKAHEKAGSLPYGEQR
                     KLEIVRAMATDPHLILLDEPAAGMNHSETHHLMETIAKIRQDFKLTILLIEHDMKLVM
                     GICENITVLDHGTKIEEGPAKKIQTSEKVIQAYLGVES"
     gene            8093..8809
                     /locus_tag="VIG33_01245"
     CDS             8093..8809
                     /locus_tag="VIG33_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8269485.1"
                     /translation="MRSKILMRVQNLEVYYGAIHALKGISFEIQEGEIVSLIGSNGAG
                     KTTTLRAICGLADARGEITLESKSLLKIPVHERVHHGLAQSPEGRGIFQNLTVFENLQ
                     MGAYSRKDKANIQKDYEHCFSLFPRLKERIQQIAGTLSGGEQQMLAISRAIMCKPKLL
                     LLDEPSLGLAPKIVAQIFEIIKKLNSEGMTILLVEQNANQALKVADRAYVLETGKITL
                     SGTGQELLSNDEVRKSYLGV"
     gene            complement(8806..9222)
                     /locus_tag="VIG33_01250"
     CDS             complement(8806..9222)
                     /locus_tag="VIG33_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269486.1"
                     /translation="MKKIILIITLGFVFSFLGCTKQSIKNRALFLSVEKFDNEAQVAG
                     KENFVDAEQQNVFSEFIKVNTRIDVSEVELQNENEATARLTVETFPKSLYSTLKTISG
                     KEWKEKVKAAKERKTYLLKMKKTNGDWEIIEQKEIP"
     gene            complement(9345..10628)
                     /locus_tag="VIG33_01255"
     CDS             complement(9345..10628)
                     /locus_tag="VIG33_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEY8269487.1"
                     /translation="MGILSSLYTGVSGMSAQGEALGVISDNIANANTIGFKASRAEFQ
                     DIISKNLKGILGGNQIGRGVKIGAVNPILSQGNVDSTEKVTDLAVSGDGYFKVKGSDG
                     ESFTRDGSFHFDREGYLVTNDNQRVQGFQADEKGNILNKMADIKFPRALIPAKGSTEI
                     KLDLNLDSRMEATKKFNEKDPYSTSHYSTGVEMYDSQGNKHLLSMFFNKTADREWSFH
                     GLVDGKEISGGEDGAMSEVCEGKLTFTVDGKLDSQELTKSAFNFKGGALQDQKIKFNF
                     GDAIKDGGKGLDGTKQYGKNSDLISWNQDGAAAGTITSLSFNDEGNLTAIYSNGQASD
                     LAQIALAKFENPEALFKVGNNRLKESRDSGGPSMGAPGASGRGKLFAKSLERSTVDLA
                     TEFVNMIQNQRGFQANAKTITTTDELLNEVIQLKR"
     gene            complement(10719..11153)
                     /locus_tag="VIG33_01260"
     CDS             complement(10719..11153)
                     /locus_tag="VIG33_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02530 family flagellar biosynthesis protein"
                     /protein_id="HEY8269488.1"
                     /translation="MKKLTGFESLRPTTPTGAKGPAGKVGGNITGDLPSFRETLDGLG
                     TLQGPAKQEVARIPIKDGIKFSNHAVERMRTRGIAFSPEDISRLSEAVGKAAAKGSKD
                     SLILMNDSALIVSVKNNTVVTVMDKTALKENVFTNIDSTIVM"
     gene            complement(11160..12143)
                     /locus_tag="VIG33_01265"
     CDS             complement(11160..12143)
                     /locus_tag="VIG33_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HEY8269489.1"
                     /translation="MGTMGVKLGTKAFGEIQGGPSTSEMNNTAKNLSVKDQKTFGDQS
                     VGDVLNKLSDPNYVDPAKKTRTVGNDKLDKDAFFKLMIAQMKNQDPTNPLKSHEMAAQ
                     LANFSGLEQMQNMNKTLTEMKLGQKPQENYQAINLIGKAVAGDSSKVTRSIGDKEHEF
                     KFNLPMDASEANIKVFNAEGEVVRSYDLKALKQGENKISWNGQDDRGQNMNPGEYKFA
                     IEAKAGEKKIAVKTDFDGIISGINYTNDGPVLLIGNQTIKMRDVRKITDPSLMSNDQK
                     ATDVTAQDLKKGTTTGETINVSPSVAVAKSNIMDNVGLSREMMSKLAKEIK"
     gene            complement(12169..13965)
                     /locus_tag="VIG33_01270"
     CDS             complement(12169..13965)
                     /locus_tag="VIG33_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEY8269490.1"
                     /translation="MLQSIVGPPLKGVADFKSSENPKAENSNEEKKAGSDSFGQVLRR
                     DETKPMTPKMAELQRPASEKAPNKIEGAEQEEQRRPESDEKVEKITATYDKGVEKKDK
                     SSSQRQNAILNFMDSFESEFGVPPTRIVEAMANLQPTDQAEAPEATADKVIGQLGLDD
                     QQEDLAKEMYVGLLAQLSQINQNAPQASPPPKAFVQSEVRISAQEKRQALNDSLDNMN
                     KKFWMNGENGSPTIAGKEDLVDKLTQLNMQEKLRQSQGLPMEEELKNGKFAEMVNQTP
                     QSGLQNQVSSPALNQASNQVLNQALDAVPMEEAEASEDEEEPTAAVKPLQAKPQMKGA
                     SANAVGESFQDDTKNFEFAKNRQNEEGFHQPQGNSTKGEMVEKTKNVEKAEFKKTLGM
                     EDIQIQPHLKQDIAMGVAAGGGAQAMATPSKAENEANIRQIMNQAEYLIKKGGGEMKV
                     EMSPEGMGKINMKVLVQDGKVNVQMSAETHEARKTLENGLSDLRNSLAAHKLSMDHIR
                     IDVVSGTNTENSSQNQMNQHQSSRDQTRQFWNTFNQNFGAPNQQRDGFTDLPNLRGYP
                     RRRTDKGLEPVQTASVNKYTGTGKGAGLNLVA"
     gene            complement(13993..14775)
                     /locus_tag="VIG33_01275"
     CDS             complement(13993..14775)
                     /locus_tag="VIG33_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269491.1"
                     /translation="MKNNGYDQFFKNARKAANHPFANSKVRFKLSEEDIEKQLKLRMG
                     MTGTQKRKKNKSIPWKLMVVSFLGLIAAAVGVQNFDKVERYVKRVEISFLGSASAEAP
                     AKPKSKTEAPAVAKADAPAAKVKKTLPEDEIDHLAKLNERKKELDEREEELARVEQEL
                     QTQKEELDKRLKDLEEVRRGISSVLEEKVKGDDKKIDTLVQMYSDMKPPAAAKVIETM
                     DEDLAVDILGRMKKKNAADIMSLLKADKAQVLTEKFAGYKRK"
     gene            complement(14848..15300)
                     /gene="fliJ"
                     /locus_tag="VIG33_01280"
     CDS             complement(14848..15300)
                     /gene="fliJ"
                     /locus_tag="VIG33_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEY8269492.1"
                     /translation="MKFKFPLQKVLDHRKIKEDLAQKDFHDVLNELQILQKKLQELEV
                     ALHEAHVRSGSLQSQGGTQGPALSQINEFQKNQKILIQLQKTKILAQEKVVEGKRELL
                     LQAAVETKTINKFKEKKFNEYREEREADEQKEMDEQNILRFKPFSKRE"
     gene            complement(15316..16644)
                     /locus_tag="VIG33_01285"
     CDS             complement(15316..16644)
                     /locus_tag="VIG33_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HEY8269493.1"
                     /translation="MNAYQLNLDKYADLLNTIHLTKDSGKVTEVNGMIIKGYLPGASV
                     GSICSITPSDLERSFLAEVVGFKDKHVLMMPLSDMRGVALGSKIVLTRQIATVKAGEE
                     LLGRVVDGLGRPLDSLGEFENFKEVRLYNEVRNPLERHPIREPLDIGVRAINATLTTG
                     QGQRIAIMAGSGVGKSVLLGMMARYTSADVNVIALIGERGREVREFIEHDLGAEGMKR
                     SVVVCVTSDQSPLLRMRGAFVATAIAEFFSAKGKNVLLMMDSITRFAMAQREIGLSTG
                     EPPSSKGYTPSVFSTLPKLLERAGSFEGEGSITGFYTTLVDGDDMNDPIGDAVRSIVD
                     GHIVLSRSLAHKGHFPAIDILQSSSRVMKNVSSAEHVKMAQKLREILATYRESEDLIN
                     IGAYKAGSNPRIDKAVKLIDGVNQFLKQDVEDRANFNQCLRQMQGLLANY"
     gene            complement(16666..17439)
                     /locus_tag="VIG33_01290"
     CDS             complement(16666..17439)
                     /locus_tag="VIG33_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEY8269494.1"
                     /translation="MPWSKAILPKITAQEKVFEYIPKKFELGTPAQALDYLEQKKQGA
                     DFRLSEALRIQTGVSEIEKATEEEKIESRALELLKEIQESAYKEAYKLGTTEGHHEAF
                     TKFSREIEERLTELEGLLNSIEKAKSEILNFNEAHIVQLAFHIATKLAKVEIEKNNES
                     LIEVLRGAVTLAQDEEDVTVRVATEQHEFIEELKKQNSRQFEFLKRIKFEPSEEIAKG
                     GCIVETNYGEVDARIEQRIQSLWETLAENVPKVKSKIAI"
     gene            complement(17426..18460)
                     /gene="fliG"
                     /locus_tag="VIG33_01295"
     CDS             complement(17426..18460)
                     /gene="fliG"
                     /locus_tag="VIG33_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEY8269495.1"
                     /translation="MRLSKSENIEFESLRGFDKAAILINYLGKDALSVLLKRLDDGDI
                     RKLINQMTKYRVVPVHVTKRVLEEYYEMVSEAEDYIFTEQTSNKDVIVEALGEERARG
                     ILGGLNISTGGNRSLESLELVDAKSLATFLVNEHPQTVAVILAHLEPEKKGEVLKRLP
                     EALQAEVVLRMANLEHVDPELIVEIDKVLKHQLANTATVEQSALGGVQPVAEMLNVMD
                     KNTETSIMSRLEEKDPLLAEEIRKLMFVFDDISKIDDRGIQALLKEIPNEKLLLALKT
                     ANEDIRAKVFKNISARAAEMLREDLSNMGPSRLSDVEGAQQEIVNAARRLEAEGKILI
                     ARGGSEDAMV"
     gene            complement(18457..20109)
                     /gene="fliF"
                     /locus_tag="VIG33_01300"
     CDS             complement(18457..20109)
                     /gene="fliF"
                     /locus_tag="VIG33_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEY8269496.1"
                     /translation="MNKIFGGIIVQFREFFKNLGPTKRLSIVAATGIVVITLIIMVFM
                     ASGKDFVPLFTNIPSDQITTIMAKLTEKSIPYQLRDDGKSISVPKELLHSTQMTLMSE
                     VGSAKMGSAGLELFEKQDFGINSYAQKINFQRALQGELMRAINTLTAVKQSKVILALP
                     NKKTFLEEGGQPTASVVLQLQQGKELSADQIRGIRYLVANAVEGLDADRVTVLDERGK
                     VLTRKSDGTVAGSDELMDLKQKVEGELQERIESILSKVVGHAKVIAKVDATLNHRIVS
                     SVEESVDADKTAIRSQQSEEESLDGARTNPAGVPGSRANLPGAEDAGQVGFKQDIKKE
                     IKTINFEVPKTVRNIKEAAGNLERISVAVVIDGKIETTTKEDGTPESKYIPRSAEEIQ
                     KFESLVKNAIGFNAARGDNVKIENIQFQAEDFTESEKLITSLERKKFIHALFKWALLG
                     FSLVLFFFIVVRPFMQWITDSFQDSVEEMLPRTIEELEELQSVDNSLPGMGSALPVLQ
                     ESIDPEKAESELLKDRIMNLLNTDEEKAGNAFSMWLVRKDDR"
     gene            complement(20135..20464)
                     /gene="fliE"
                     /locus_tag="VIG33_01305"
     CDS             complement(20135..20464)
                     /gene="fliE"
                     /locus_tag="VIG33_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEY8269497.1"
                     /translation="MAGFTVSSASNFLDTGKIRDSKSLSIDQPMSQSKEAQGSFADTL
                     KSAIDNVNQLQKVSDKSAQDLATGRTDNVADVMIAAEKADIALRVMVQVRNKIIDAYQ
                     EIMKMQV"
     gene            complement(20556..20999)
                     /gene="flgC"
                     /locus_tag="VIG33_01310"
     CDS             complement(20556..20999)
                     /gene="flgC"
                     /locus_tag="VIG33_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEY8269498.1"
                     /translation="MADFFTGMRISGSGMAAQRMRMNTISSNIANINTTQTPEGGPYR
                     RKDVVFEAMPDAKSFGEIVSPTSAKADFQRVQVSDIISDRKAPMLKYEPDHPDANSEG
                     YVAYPNINLMEEMTNMIQATRSYEANVSAMQATKDIALSSLEIGR"
     gene            complement(21020..21424)
                     /gene="flgB"
                     /locus_tag="VIG33_01315"
     CDS             complement(21020..21424)
                     /gene="flgB"
                     /locus_tag="VIG33_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEY8269499.1"
                     /translation="MSNLFDKTTNALATSLAMRQLRHSVTTSNIANAETPGYHAKKVD
                     FEAALGRALDIDGLRGMSTSSADHISVGGASAAVRPDIYENPEGAINNDGNTVDIEKE
                     LSALSENAILYKTALQLINKKMAALKYAIGDR"
     gene            complement(21443..24349)
                     /locus_tag="VIG33_01320"
     CDS             complement(21443..24349)
                     /locus_tag="VIG33_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8269500.1"
                     /translation="MSLSDTDKNQWPIFSQLENICYRAHFCLALYVQCTMDMKLSFIV
                     VSLLMSLVFASASVVWAARVSSSISDQGDTVHLELSGQQNWDYDLRRVKKSDKSYLQL
                     TVDAIDEASIRKLLAFKSEMVKAVALEKEGPDGKFILNFLVADEDVESFDYLTDQPSR
                     LIVDFYVNAQNKVKSVTATAADDAVETTVLPAKTAKVSKTAEKTKTEKTRKPATTDIL
                     QIKSDGTNSSNLAESRSGLFDGGDPNFERFMIKDYEIKDDALLRSKESYFIPYPMIHP
                     ENHYWEKMKIASPIYEIVSKSTEENKQARLLLTLFENKRYAVYIKSEEWFQKKYPESE
                     YNDLIDFMTADVYKNLWRQKQDPKYYEIAQIKYKQALQRHPQSPLVERTSLMLGNMAA
                     EKEDPLDAIRLYNTHIEKNYAGKPDAISNELARIGIGISFLKLNRYQEASDIFSKLQK
                     ESPLKEIRAEAAYREGDVYVQAKNYQKAVDTYKEAIKKFPEHQSEYPNAFYNQAEALF
                     GMGNYYQALGVYLEFMKKFPSHEHAALAMTRMGELLDILGADKSRVVGAYLETFFRYG
                     ENNNAIIARLRLTSARMKSMKPKEVEVATKEILSLMKKLDIPNIEQFGTVMIAEGYTS
                     RGEHEKAIDLLTDYYQQNPTVVDRNLLTSRIVGNISDEIHAQVNAGNFIKALKTHSKY
                     AENWLKNNDRIDTKFYLAQAFEMAGVPPEAEKHYRDVLNKVYAIKGTIRDKELTATKR
                     KPDEDELNLRLAVTTFQQKKFNQSYDYLKNIKNPEGLSETQQIERVRVAVDLLEKRGD
                     LDSAVRYLTELLKTWKGQARLVAEPYFRLAQLEVKQGKPEAAIESLKKIDLLMTDSKS
                     VSEAVHEKGLELLGQLYLDKGEGSKAISAFDSLLNQYEEKRPLASIRYKMGQIYFKKG
                     DVQKAADVWSKLKSQKSQFWYNLAQEQLKNSEWRGEYKKYIQRIPAMSQQQTQQ"
     gene            24458..24970
                     /locus_tag="VIG33_01325"
     CDS             24458..24970
                     /locus_tag="VIG33_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269501.1"
                     /translation="MKNHVLRYLVLTGCVLECLLGIGACVSVKLPSSMRTPAKDVQFQ
                     VPEKPFQEIKVLTADKAWLSAETGNTISFLSECNGNVDPTLEQLQNESLTVLDKLKIL
                     QAKNLEFNGREALRTEAQGEVDGVPVKMELLIFKKNNCNYTLSFGGVLKRFNTEKKYF
                     DKFTESFKAP"
     gene            24970..25752
                     /locus_tag="VIG33_01330"
     CDS             24970..25752
                     /locus_tag="VIG33_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8269502.1"
                     /translation="MDALPVATLLVEIIRFWGGLGILTGQVLRETFKGKIYWKLVLEQ
                     IYQVGYRSLPLILITSGSIGMVMALQFGLGLEKFGGKLYVPKLVAATILREIGPMFTS
                     LMFAARIGAGMASEIGSMMVTQQIDAIRALGTSPIKKIVIPRVLACLFALPVLVAFAN
                     IIGNLGSLLVGATELNLDPHFFYLKIMSTASIDDYLSGFGKTFFFAIFVSLPSCYFGL
                     NVKNGAKEVGIATTKAVVVSSILILVGDFFLSKVFWMIAKWV"
     gene            25743..26474
                     /locus_tag="VIG33_01335"
     CDS             25743..26474
                     /locus_tag="VIG33_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8269503.1"
                     /translation="MGVIDVIHFKKSFGKKVIHQDVTFSVQKGECLGLIGGSGTGKSV
                     ILRSLIGLEKPDSGRVIIDGIDITQMTEPQLLEIRKKVAYAFQNGALFDSMTVFENLA
                     YPLREHFHYSEKEIHERVLSSLAEFGLEGNENIYPGSLSGGMQKRVGLVRAMMMNPQI
                     ILYDEPTAGLDPFNTRKIQDLILRLKEKGVTSILVTHDMPTADAVCDKMAMILNGRIE
                     AQGTLSQLDQDSLGVITKFIAGEEL"
     gene            26483..27286
                     /locus_tag="VIG33_01340"
     CDS             26483..27286
                     /locus_tag="VIG33_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY8269504.1"
                     /translation="MENNLKTQFRVGLFLSIGLILILGSIFMLGGDRAFFKRYTTLHA
                     HFEQVQGLAEGSMVSFSGITIGNVKKIQILPERNLIDLVLKIDNEFMPRVTEGSQVEI
                     RTQGALGDKFVFIIPGDPKAKPLADGSTIEVAKATDILGILSERGNEAEKIFDIINEM
                     YHFSKTLNAENRTDKILSSLASATTKMDKTATATQKIAESFSDGKLKSTLDHLESVIT
                     KLDRGQGTLGALINDPSVHEQIKSMLGGSNRKSYIKSLMRTSIEKTEQK"
     gene            complement(27309..27710)
                     /locus_tag="VIG33_01345"
     CDS             complement(27309..27710)
                     /locus_tag="VIG33_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEY8269505.1"
                     /translation="MRERDPKTKFSLGSEVLQKLFEGGNSPLSEQFMRWKLWAHWGDF
                     AGPTIAQNTEPVGFNRGVLYLWVRSSPWMHQLSFMKDHIQETLNKKLGKPVIKNIRFT
                     LDRREVPSDNESQNNFRNVIQKIAPENEDKE"
     gene            27814..29169
                     /gene="trmFO"
                     /locus_tag="VIG33_01350"
     CDS             27814..29169
                     /gene="trmFO"
                     /locus_tag="VIG33_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEY8269506.1"
                     /translation="MSLKTEKKITVVGAGLAGSECALQLAEMGYQVVLFEMRDKTMTP
                     AHKTGKFAELVCSNSFGSMGEVSAPGQLKWEAQKLNSKILEVARFAQVPAGQALGIDR
                     EAFSSKVTEMIRNHPNIEVRNEVINNLEEVPRPAVIATGPLTHPPLAESMCSHFGNEF
                     MYFFDAIAPIIDTESINTEIAWKADRYDKGEGGGDYFNCPLNKQEYERFIAEIKAARK
                     IEPKDFETTAFFEGCMPIETMVERGDQTLRFGPMKPVGLDNPKTGRYAYAIVQLRQDN
                     KEGTAYNMVGFQTRMAYSEQIRVFRMIPGLENAEFLKLGSIHRNLYINSPKSLNCDLS
                     SKRDPWLFFAGQITGVEGYFESTCVGLLVAKFLDQKLRDQVFNPPPRASAMGSLLEAI
                     TDPIRASHFQPTNINFALIPPLTEVEIEELKLQKLHRDKKAKKEIQIKKAQTALVEWL
                     G"
     gene            29259..31958
                     /locus_tag="VIG33_01355"
     CDS             29259..31958
                     /locus_tag="VIG33_01355"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEY8269507.1"
                     /translation="MSEKSQSQNALSDRYNPQEVEGRVYKWWEEGGYFKAENRSTKPP
                     FSIILPPPNVTGFLHMGHALDHTIQDLLIRWKRMNGFNAMWLPGTDHAGIATQSVVER
                     ELKKTEHVTRHQLGREKFVEKIWDWKNQYGDRIYSQMRRLGDSCDWERATFTLDEGVS
                     KSVRKVFVSLYKKNWIYKGQRLINWSGPLETAISDLEVEYKQVKGSLWHINYPLEDGS
                     GFLTVATTRPETLLGDSAVCVNPEDDRYKHLIGKNVIVPLINRNVKIIADTYVDETFG
                     SGVVKITPAHDFNDYKIGKTHNLDFINILTKKTTLNENAGPYQGLTVIEARKKVVEDL
                     KNQNLLVKEEPHVNNVGHCSRSGAVVEPFLSEQWFVKTEGLAVPARRVAESGTIRFEP
                     ESWTKVYLHWMNIIEDWCISRQLWWGHRIPAWNCADCGHVTVAETDPTECEKCLKKNL
                     SQEEDVLDTWFSSALWPFSTMGWPEETETLKTFYPTNYLVTGHDIIFFWVARMIMMGL
                     EFKRDVPFRTVYIHGLVRDSQGKKMSKSAGNSVDPVEMIDKHGADALRFSFLAHLYSG
                     KDFKFAEQRLEGYRNFMNKIWNAARFALSHLQDFQVPEEGVKALPNKLQISVFDQWII
                     TKLGEVEKTVEEALEQERFSDAATALYQFTWNQFCDWYIEFTKPVFSGSNKEERAATQ
                     LVMAQVLNRMMRLLHPFAPFISEEIYQKLPIRGKACVVDQYPNLRNDKDFLALGSPQA
                     AYEIDIVKDVVTAIRNIRGENRISPAEKLNIRLGIEEAEMQKILGNNRNAILTMARLG
                     NLEIGEDGNLQKCAVAPITTKRGQIKVIIPLEGLVDFDEEIKRINKTIEKLGKDISLL
                     TNKLSNEKFIANADEDVVAADKELLVQSKLQIESMRAALIRFQ"
     gene            complement(31981..33087)
                     /locus_tag="VIG33_01360"
     CDS             complement(31981..33087)
                     /locus_tag="VIG33_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269508.1"
                     /translation="MGVEKLNTLVCNVSNQDWMSLIPKDYFVNNRILFVFVMLFTQYI
                     FADQNISWPENNSYSQLTSTFRGSTTFQDFSKKVEKIGFTSTTILPESSEVWTPISDG
                     GPISLKDAYFSRMLHTYYQSYRKYKALDSVSKKTLLIKSDLKAAEKDFSTATQNLKLY
                     KQAYPNEFSKLIERVKFPANEGSGWGFTLKSLADIREDGLESDDPLLKLLTDPNGIFA
                     DQNLSIAKLSSTNVDGKSLVNGESQSKSEEKINAPIKDKEGCNLKAMDAYKREIIRVK
                     SGSYYGVSRNNGILTSKTPCLLLDLSFKISKNPFKRTLLGGNIMDMNGTIKSISQSEF
                     DSVLSHWGILKNPVPDTSLSNKYKPSSREMKGAQ"
     gene            33503..33766
                     /locus_tag="VIG33_01365"
     CDS             33503..33766
                     /locus_tag="VIG33_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8269509.1"
                     /translation="MKKKRTVPSKRRTKEELKSDLPTISLFVRNRREELGYTQEELAF
                     RAGLSVRFLKEFERGKKTVRLDKVIELLEFLGATLEVKARSRE"
     gene            33763..35082
                     /locus_tag="VIG33_01370"
     CDS             33763..35082
                     /locus_tag="VIG33_01370"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEY8269510.1"
                     /translation="MNPKGKVYFKNQLSGVIEKNEDGSFLFSYESHSPSVSLTLPTSK
                     THYQSEELFPFFDGLIPEGWLLNLVQKNWKIPKNDRMALLLTTCQDCIGATHVLGEQD
                     NTTLSKSHAKTSLNHREAHTFTSDRCLVCMEALNSSEQLYHSKCAKNLFGFSHAPIID
                     VVSEDLEELALRQINQRLTLTGVQRKLSLSPIIENKKERLTVVGVDGRFILKPASEDY
                     PEMAEVEHLSMKIAELQNITVADCGLVYLASGERAYITRRFDRANNEKIQVEDFCQLA
                     EKPTERKYSGSSESLAKIIEKYSDQAPDDKLTLFQIILFSFWIGNSDMHLKNFSLWRD
                     PQSGLIRMSPGYDFLSTRLLITAKEDNEELALPVNGKKNKLRWSDFVALASNFKIPPK
                     VFMRIRDQMLDSFFDAEDLVEKSFLSYQKKEKFMDLLRERSERLAQK"
     gene            35234..35911
                     /locus_tag="VIG33_01375"
     CDS             35234..35911
                     /locus_tag="VIG33_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY8269511.1"
                     /translation="MEFFLSLGRAFTSSDAIWMWAILSAQIVSVAIIIERAIALFVKR
                     KDNQKSLSKLIAEDIRAGNLDKALRRSQQIGSKDPLGVVAAAGIQAAIDMGGKEEIQL
                     KMDEVLLEETTRVEKRIGFLAMFANVATLMGLLGTITGLINSFAGIANANAAEKASIL
                     SKGISLAMNTTAYGLLVAVPALIMYAVLQNRASRLTDDLNKAALNLFIQLGFHYSPVT
                     DKKSKGN"
     gene            35963..36406
                     /locus_tag="VIG33_01380"
     CDS             35963..36406
                     /locus_tag="VIG33_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY8269512.1"
                     /translation="MKKELNFELNILPVLDILSVLICFLLLTAVWIQLGTIDTKQAIG
                     DNSTAGAKNPPSLWVTLGKDGTVLLSLRDLPQAKNLEESIAKIGSGVNWTLLEEKIQD
                     LRNKWPELKTSIVRPEARVSYGDVIHIMDQLKKNKFDGVGLSPLG"
     gene            36413..36895
                     /locus_tag="VIG33_01385"
     CDS             36413..36895
                     /locus_tag="VIG33_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY8269513.1"
                     /translation="MKTAAFISQRRSPLLQALSLKAGEKSASHTKELGLALPLTSLID
                     AFCIIVIYLLIGTQTSGVESQLPGKINLPNAEHGQTLEKPVATLRIEKGNYFLNEKPV
                     DRASLSAKLSHLKKESTEATELMVQADTQMEYAELDPLLKAGSEAGIEKLRFAVIPSK
                     "
     gene            36892..39912
                     /locus_tag="VIG33_01390"
     CDS             36892..39912
                     /locus_tag="VIG33_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269514.1"
                     /translation="MKTLKHFLIFSAIFGWTLLAGISIAYAEKMNTQTQDMVIERMER
                     IVSAMEKSDKSWLPSQQRLADLLSERARLRFMQEVEANCDACKGSKADRLKAVSIYEN
                     LLQEVKVNDHGPILFQLAHLHEMAGQPDAAIALFERIIKEAKAKKISKDIVSHSQASL
                     GDLLFQKGKFKEAYTHYKISLKDTNLKGRGLVIYNMAWCEFNLDKLNSGITTMEGLLK
                     RPDLIVRENSDGSGEANGTKYDPVFHADIVRDLATFYARRPITNKEITSYETFAPKEK
                     RKELLLLFASEADRLGQKKAAAAIYNHYLEDTTLSQEERLEAFVKLAQVNYDGGDATK
                     STQDFAKAALEFQKNCKEAEKCQELGKTMKHYVTEIHRAKKLKPDADLLNSYLIYNKT
                     FPNDTEMAQLGAQVADDMGKHAIAMQFLRTVSDRKSALPQSQQTALVSELSVAEKSKN
                     PVLQKAAYEHYLAVSPQGNKSFEVRYQMAYMTYQQKNYREAAQAFNTLAMDKSGSAEL
                     RKKSADLSLDCLVQMKYEDSLEDWAWQYSTAFPQAKAEYETMARKALLNRVARIANDK
                     NSSNGELKKALQQMQSANMASAKPADKVIFYTNLSVLAQRLGEEGILINSLQTLVSLP
                     GVSADRREESLGQIVSYYEKKLDFKNAYHTALRMKFSKISLKDKELRLGTLADLATLK
                     PQKHYHASLNAGLKGESARSVRSRLVLLSTNPTRELKAQADELAKDPGLLNEITLLVY
                     ARTGDGKALKPVLARRELHSRTAPNFIDKQGFYKEVASFKEQISRHQLSSRSDSSLQR
                     TIKERVKLLKSTDTFLANSLKFKDVTAQLMVLNMISTENERMVRDLVALPIPPKLTAS
                     EQKQYLDILKLKSKPYFMKAKVAQQRESEVWDHSPAIPQLLKDYESVRPELKKYLHRE
                     LTLLAALSKDKSGSVNNGKMRSRIVDALNENSSSTWSNQWSDLVNARKNVAENPESMS
                     DIEKLKNLETKIGHPLMPSYLEARLSRLQRGKTL"
     gene            39909..40187
                     /locus_tag="VIG33_01395"
     CDS             39909..40187
                     /locus_tag="VIG33_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269515.1"
                     /translation="MKFTITLASLLLILSTALTVHAGTATMVKKHSKKNSQAKLGTSF
                     KFDGSNLRGKYQNSLSTTATVENDKFLEDLLGGRTQFEDRDQKERERN"
     gene            40200..41441
                     /locus_tag="VIG33_01400"
     CDS             40200..41441
                     /locus_tag="VIG33_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY8269516.1"
                     /translation="MSTARKFTQLLILENTLGQKVRTFAVDSRSLNIVYLKDQRRIDA
                     VANLKTLEDRDLEYILLKTFEVKDLDSKALKLQGLGQLRMMDVNAVDSPDHQLAPEKD
                     KKELKVILEKTFISHILLVLILMMGAWISTKFFSQKKEEPALVTIVMTKPEPKIEKAT
                     PAPHVKVSEKKIKPTPKVYRPVVQKKLLTKPYKVKTAKATDVQRVGALAALGGLAKGH
                     RGAEGLDMNSLKNIRSAGEGQGGGGVGATGRGGVKGMMPGSGLIAGSAGEGGRAESAG
                     GYGTRGVGGGRAGYGKHAIVGGMTPVSLPLDNEATVEGGLDRDQIIAVINKNKGQIIY
                     CYEKGLQAQPSIGGRVAVDFTIGPSGRITLAKVAQTSLGSKFVENCMLDKMRTWQFPK
                     PVGKVNVDVLYPFELTRVSSR"
     gene            41451..42560
                     /locus_tag="VIG33_01405"
     CDS             41451..42560
                     /locus_tag="VIG33_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269517.1"
                     /translation="MNNFLRSSLLSALLLTTACGGSSDNKSFEDISELRARAKKEMTT
                     GPQPPPERVVYVTKEKEVIKEQATLTDSYFKIDLSEKMMSFYEGQPSAYKITLRVQDP
                     QLQMKLTAKGLPSGAELKDISTAKNPNTYELRWTPPLYTISYDQQPPQIMTATLVPVL
                     VSAKTSKKAETVKGLSLDRTITFNVFRNQDKPSELVLSGLDQDVQEGQVVPFSVIARF
                     PGVDTNAPIKPDLGFFRDKNTQVVASNYLEMDGTRYVSIENVEYIGDYKWKFNLVFDT
                     KNNPVETQKSKDGSAAAVNFTQVRLGLRVFGAFNASPATVVRVKIIRAATEATPVAPA
                     PAPAPAPPKDPAPSKPTPKKAPKPKSPTKPKSGGK"
     gene            42563..43339
                     /locus_tag="VIG33_01410"
     CDS             42563..43339
                     /locus_tag="VIG33_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HEY8269518.1"
                     /translation="MKNLKASTLGTLLLISLSVSWNASAASKATPTSPATPAAQAKEI
                     NFSKDVDGLGGNDALMEMAEKLNPESKSRIVQNRLVDRHTRLEAGIFYGGVFGGATYL
                     QTQSYGATLDFHITPRWSLGARYYQYQNKLTPEGERAFDEARANYAAGSRATLVDIDS
                     PLNAKIAVINWYPIYGKINLFDAAIAQFDIYLLAGGGQIELESGNSPLATGGVGFGLW
                     MTKHLSARAEFRYQAYQDEIVTGQRDINAMVATVGLGWIL"
     gene            43336..44361
                     /locus_tag="VIG33_01415"
     CDS             43336..44361
                     /locus_tag="VIG33_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269519.1"
                     /translation="MITLSIFKQLALALVIGMAHAEDRLSAQKWSEDFIKTPSLDFEK
                     LDKKILGACEDCENKSKMQTARKLFAKGQYDEARALYAQVSKGSAYWLEAVEEKAWTY
                     FRQNELESAMAQAKTLLAPQFENYVGSESYFMQSLAQLKSCNYKGVFETNRLFKEKQK
                     THLAEIQALSKTGMNEALKNVMNETDKFPMRFTDVGENASHLPHLFFKDIEFQRQLMR
                     YKLSEQALAALKAEDRASALQSTLEKVKSASASQLQNRIKELAVQETNDNFKIVQKLN
                     LVEIEAIQRLHADLDLDKSLFKKGPFANTNSDQLVFVDDGRPWIDELDKYEVRTKTCP
                     QNLRRKM"
     gene            44358..45629
                     /locus_tag="VIG33_01420"
     CDS             44358..45629
                     /locus_tag="VIG33_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269520.1"
                     /translation="MKTPMKYSTLLMTALSLSFLAACSPESGSLVASKPYAQPTPPTY
                     APVPEQKSGWITEDGGQSQLDFNPQVDILFVSDNSDSMKSAQANLVRNIDKFADGIIK
                     NKMVDYHIGVVSSWDSSERFLTTKKDQYGLGELHFVKDSKNQTINKRFVSRTEKDLMA
                     STLDIGVASYADGGPENEEFFSPLVAAMEKSGNGGVNDGFFRANAQLVVIFLTDADDS
                     STALTTDELDRKLLNFKGGKRDKLSVYGVLVNANDDDQYKDWGLRIHPKYHPECFNVT
                     KKSPTRNDVCKKGFGPDRIEQLIVTANQGSGLPAKEIRKKYIMSIVSKNFGTDLATIG
                     SDIKIRTMAKEIFLSQVPVVTENQQVQVRVRYGTPKELAAGRGQLIPQSAKGGWLYNP
                     ESNSVRLSGDINYNYEDGARFAVDLMPVILK"
     gene            45632..46279
                     /locus_tag="VIG33_01425"
     CDS             45632..46279
                     /locus_tag="VIG33_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY8269521.1"
                     /translation="MRTTNILLFTLFAFSAHAQGRALSIEQVLDAAGSGKLVTLKKYH
                     SKKGDLNAQDSHGLTPLMQAVATGQKKVVDYLLQQKVNLELKNANGDTALAMAIGNEQ
                     DQIAVALINAGAKVDVLGGEDKNNLVFMAASVNATKTLQLLTKKAPEQINMKNKNGDM
                     PLHEAARFGTEKTLEVLLNAGAKKEIVNNFGKTPLDLAKSINNKAAVRVLSRTTP"
     gene            46368..>46906
                     /locus_tag="VIG33_01430"
     CDS             46368..>46906
                     /locus_tag="VIG33_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8269522.1"
                     /translation="MTYSLNKHELQQILRDQRKEIESLHSKWLPREKLHEFKSLLSSK
                     LVKVVMGIRRSGKSTLCIHALPFEQFVYINFDDERLIRLTTKDLQTVYEVMTQLNPQA
                     QFYIFDEIQNIDSWELFINRLKRKGLNIIVTGSNGQLLSKELATHLTGRHLSLELMPF
                     SFYEFLGYKNASIELNEASS"
BASE COUNT        12844 a        10764 c         9951 g        13347 t
ORIGIN      
        1 ccaacgtcat gaagcaactt aatcaaactc agcttcgcaa ttctatgtct tgtttattgg
       61 aaatcacttc agagagttat tgttccgctc tggatgccag caatctcttt aaagaacaaa
      121 tgagccgatt tcagattgtc agtggcaaag acgacacgct taaaatcact gattcggtgg
      181 atatgcaaaa tagcaccaag ggacctcttc aaggctatta catcttgact cagcagatcc
      241 cggtgatcac ctcttggctt gaaaaaatcc agcgcggggt tgagccaaaa ttaaagacgg
      301 atgctaagtt taagaatgaa actattcaaa ctgtaatgaa tttttatacc acagaaaata
      361 accttctggg ggatatcaac agcagcaaaa cgacactggc aagtcagact tcccttcaag
      421 ccaagaaaaa catggtcctg aaaactctct tatccatttc cgatagactc gctggcaata
      481 tcgcgaacga taatgaaacg aacttcttta cgatcaacat gagacccatg gaaattccct
      541 tcgctttgat tggccgggag gttccccccg aagttctcgg aaaagggaaa tccgatttta
      601 tgcaatcacc agagagctgg ttgcaagcca atatgtcaaa cttgagtgaa tttgaaaagc
      661 cggaacagct tatagaaacc atccatagca atttgaaatc gctcatcgaa gcttcgcaag
      721 ccagcgctgt gagctactac aacgagtggt tcatctacga taatgccgct ctctatgtcg
      781 attcagtcac cggcatgcga tacaatgtca gggactcctt acaaaatacc aatgactatc
      841 tttcttcttt agaggaaaaa attaaatctt ccaacagtgc ggatatctct atcattccca
      901 ctattgatga aacccgtttc ttaatcagca ccgtgctgga aaaatatgaa aacatccgct
      961 tgttagggga aacctataga gccgcaacta cctatgagaa tcgcaaagcg acaattgaaa
     1021 aaggaaaaca gcttcataaa gaactgatcg aatctgttta ctcgagcttc agcgtcctta
     1081 agggaaaatc aggatttttg gcaaatcgca tggcgatcat cgtaaaaaat gatttccaaa
     1141 ccactttaaa atccggagcc gacttttcga atgaaaatgt gaaaaaaatc ttttacgcca
     1201 ctggcgatgc cgcctttgaa aaaatgcgtc agatgtcttc tggaaacccg aaagatatca
     1261 tgatggatct taacaatgct cagatgaccc ataaagaaaa tctgaccgct attgaaatga
     1321 tgttgaaaaa ccatttcttg gctatgattt cttatactaa aatggtcagc caaggcattt
     1381 ctccgtcgga tgaggatgtc agtcgggata gtttcaaacg tgcctggagt gattattggg
     1441 aggaataccc tatcgcttcg agaaatattt taggtgtcat tcttgaaggc attatcaagc
     1501 cctttgattt ttatatttca ttttacaagg gcaaccacga tcgttaccct aaatcccgca
     1561 cgacttctct tggcaataac aagttggccg cttcggtgga caatgaaaat gaagccgcta
     1621 agcatatgta tgaactctac tgtatccagt ctttggcctt cacagactgg cgtcctttcc
     1681 aagcactttg taaagatgtg gttttgaaaa gccctttcaa tacggcgact ttaagcccct
     1741 tggctcagca aaagtttgat acttctttga atgtttccta cgattataaa cttgctgaga
     1801 acttaactga ccagaccaga aagaacataa gcaaaattcg tgaacagttg agatatgccg
     1861 ctatggatag aaatgcgggc aaaacctacc gtgaaaagat gagccaagtt aaacttgaaa
     1921 tggccaagaa tcactccgcc agaatttgcg ccctccgcga ttatggtaga aataacttcg
     1981 ttatgtattt aactttgggc ataaagaaat aatgagttga gaagcctacc gatgaacgac
     2041 cttcgcctcc actttaaaag tttccgtcaa ttttggcctt tctatgtatc ccaacatacc
     2101 aaccctatca atcgaaagct gcacgtaggc ggaaccttgt gtgggctaat tctagcgact
     2161 tttctttttt caatgggtca ctgggttctc gccatcactt taggactggt ggtcggatac
     2221 ggatgcgcat ggctcggaca cttctatttt gaaaaaaatc ggccggctac atttcatttt
     2281 cctatttata gttttatggc agattttaag ctgatctatt gtttgctcac cggacgtagt
     2341 cttgtccccg cgggagcaaa gaaaattatt tttattactg gggctggacg cggcattggc
     2401 ttcgctctgg ttcgtcattt tctaaaccaa ggacatcaag tttttgccgc agtaagaagc
     2461 tctcccccag cattagatga gttgcgaaaa aaacatgaaa ctttacaaat agtggaaatg
     2521 gacgtcacta aagaagatca acttattcaa gtggcaaatc aattagcctc cactctttcc
     2581 ggaaatgaag cgtttattct gatcaacaat gcgggaatat ccggtggcgg accttttgaa
     2641 ttgatgacga gccaacattg gcaagacatg tttaatgtca acgtgatggg cttagtgaat
     2701 gtcacaaggg tatttctacc ccaggttcga cgtacaaaag gaaggatcat taatataggc
     2761 tccatgagtg gccgtgtcgc cagtcctttt gtgtcttcct actccagctc taaatatgcg
     2821 gtgcgggcag tgaatgacag cttaagacga gagctaggac cgctcggtgt ttccgtaatt
     2881 ctcattgagc cgggtcctat cgccaccgat atctggagct cctcgatgac caacagtcag
     2941 aaaatgatgg agaatgcttc tccggaaatc ctcaaggtct atcagaaaaa tattgataaa
     3001 ctggaaaaag aaatccgtaa tattgagtcg tccacagtga gcgttgaata cctcacccct
     3061 tttgtggatg aggcggtgaa ttccccccac cctaaaccct attaccgagt gggaaaaaat
     3121 attggtttta ttttcattct gacccacttt ctaccagcct cgatcctgga ccgaatatta
     3181 ggcggacttc gttttgaaaa gatgaagtaa gtcttgggaa atggttcaga tttgcgccgc
     3241 agcgatttca ttctttacat gaagcaatgc aaattgtgta gtaagttaaa ctctgaggag
     3301 ttgcagatga aaagaattgg cgtatatacc tctggcggag acgctcccgg aatgaatgcc
     3361 gccgtgcgag ccgtagttcg tatggccatc gcaaaaaaat tggaagtcta cgcaatctat
     3421 caaggctata acggtatgat tgaaggcaaa atgtctccaa tgcaacctcg tgatgtcgcc
     3481 aatattattc aaagaggcgg cactgtctta aaaacaggtc gctcttctga tttcatgaag
     3541 cctgaattcc gcaaaaaagc tgctgacaac cttcgtgcgc tggggattga aggcttggtt
     3601 tgtatcggtg gcgatggctc cttccgcggc gcccacgccc tctgggaaga acaaaaaatc
     3661 cctgtcgtcg gagtccctgg cacgatcgac aatgatatct ttggctctga caataccatt
     3721 ggttttgaca ctgccgtaaa tacggctctg gatgccatcg ataagattcg cgatacagca
     3781 gcaagccatg accgcctttt tatcgttgaa gtgatgggac gaaactcggg acacatcgct
     3841 tcttatgtgg gtttggctgg gggagcggag gatattttca ctcccgatgc caacaccacc
     3901 gttgataaag ccgtggacag aatccgtgac tctatccaaa gaggaaaaac cagttcaatt
     3961 atcatcgctg ctgaaggaca aaaacccgga cgcgcctatg acttggccga agccattcgt
     4021 aaaaaatcag gtctcgatgc gaaagtttgt atcctcggtc acttacaaag aggtggaagt
     4081 cccacggcca gtgatcgagt gttggcctcc cgcatgggag ccgctgccgt ggaagcttta
     4141 atcaagggtc aatctgacat catgattggc accgatggac ctcaccttgt cacagtccca
     4201 cttgaacaag ttgtaaagtc cgagaaaaaa acatccatgg atctcatcaa tctcgcaacg
     4261 gcactgtccc tatagttgaa tcttttaaat agaaattgac ctgactcctt gtgattgtaa
     4321 tcatttagaa atcgataaag aggagtcaaa aacatgaaaa aattgatcat cactttgcta
     4381 gcggtattgc cgttaatagc cgcctgcact aagaaaagca ctgaaatcct agtgggagag
     4441 ttcggctctt tgactggcaa tgatgcaact ttcggacttt ctacgaataa aggtctgcgt
     4501 atggcctttg aagaaatcaa tgcggccggc ggagtaaatg gcaagaagat caaactgatc
     4561 actattgatg accaaggtaa aagcgaagag gctgccagcg cagtgactcg cctgattacc
     4621 caagacaatg tggttgctat tatcggggaa gttgccagcg gccgctccaa agccgcagct
     4681 cctattgcgc aaaaatatca aattcctatg atctctccgg cctcaacaaa tcctagtgta
     4741 actcaagtgg gagattatat tttccgtact tgctttatcg atcccttcca aggttttgtg
     4801 gttgcgaagt ttgcgacgca aaatttgaaa tctaaaaaag ccgctttgtt tcgtgatgta
     4861 aaatctgaat actctgtggg cttagccgat gtgtttgcag tcgaatacaa aaaaatgggt
     4921 ggagaacttg tcgctgacct cagttatcaa ggcggcgata ttgattttaa agctcaactg
     4981 acccaaatcc ggtctaaaaa tccagatgtg atctttattc cgggatatta taatgaggtg
     5041 gggcttattg ctcaacaagc tcgtcaatta ggaattaaag cccaattgct aggtggtgac
     5101 ggatgggatt cctcacgact cagcgaaata ggtaaagacg ctgtcaacgg gagctattac
     5161 tccaaccatc ttaccaacga atcgacagag cctttggtta ttgaattcgt gaaaaaatat
     5221 aaagcgaaat acaatgagac cccagatgta atggccgctt tgggttatga tgccgctaag
     5281 attttagctg aagcgatggc tcgaacaaaa gagctgactt caaagaatat ccgcgacgaa
     5341 atcgcgaaaa ccacagactt ccctggggtc acgggaaaaa tcaccatcaa tgcggaacgc
     5401 aatgctgtga aaagcgcggt catcgtgcaa gttcaaggtg ctgaacgtaa gttcattaca
     5461 accatcactc cttaaggata tccatgcagg attttataca acatttgatc aacggaatta
     5521 gccttggaag tatctacgcc ttaatcgctc ttggttatac gatggtgtat ggaattttgc
     5581 agatgatcaa cttcgcccat gccgatgttt atatggtagg agcttttacc gcttactatg
     5641 ctgcgaaatg gatgggcctg gaaaattcgc ctggcatttc cactttaatt attttgcttt
     5701 ttaccgctat gtttgtttgt agtcttttag ggcttgcgat tgagcgtttc gcttatcggc
     5761 ctcttcgccg ttcccctaaa ttgaatgttc ttatcaccgc aattggtgtg agtttgtttt
     5821 tagagtttgc gggccaagtg gtcttcggtg cggacccaaa agttttccct gaagtgatga
     5881 aagatttcgt catcagcagc ttcggcggta ttgatttgaa atcttctgat gtcacggttc
     5941 ttggagtcag tttggtggcg atggtccttt tgaatttcat tgtgcaaaaa accaaaatgg
     6001 gccgcgctat gcgtgcggtc agcaccaatg ccccggtcgc agctatgctg ggtgtgaata
     6061 ccaatcgaat tatttccttt acctttatcg ttggttcgtc cttggcggcc atcggtagca
     6121 ttctggtcgg gatgaagtat ccaaaaattg atcctcttat gggtatgatg attggcatga
     6181 aagccttcgt cgccgctgtc ctagggggca ttggaaatat ccaaggagcg gtactcggcg
     6241 cgctgattat gggcctctcc gaggaaatgg tggtcgccta tctttccagc acctaccgcg
     6301 atgctctggc ctttgggatt ttaattgtga tcttaattgt aaaacctacg ggtctactcg
     6361 gtaagtatgc ggtggagaaa gtataatgaa aaccttcaaa gctccgctac tcagtattat
     6421 cggcttatta gttattggtg gcatccttga ttttggagtg aacccttatg ttcagctcat
     6481 ggtcctgtat gccatcacca actcaatcat ggccatgagt ctaaatctgg tgaatggcta
     6541 taccgggcaa ttctctctgg gccacgctgg atttatggct atcggagcct atgtctctgc
     6601 tttttttagc actcactttc ccttctttgc aggacctgga attatcttaa ccaccctcgg
     6661 gggcgggctt ctggcggcat ttgctggttg gctcgttggc cttccctctt tacgcctcaa
     6721 aggggattac ctcgccatag ttactctagg ttttggcgag atcatccgag tagccctcct
     6781 aaatatagat tcactgggcg gtcctcgtgg aatttctgga atcccgtctt ttccaaactt
     6841 ttttatcagc tatagctatg cctgcgtttg ggcggtcatt tgcttcttcg ttatttggcg
     6901 tttagttaaa agcagccatg gtcgcggctt tttaagcgtg cgtgaagatg agatcgccgc
     6961 tcaagccatg ggaattaata ccacaacaat gaaagtgcgc gcctttgttc tttctagctt
     7021 ttttgcggga attgcaggcg ctattttttc tcatgtcaca aattacatta gcccttccac
     7081 tttcactttt ctattgagtg tgaatgccat cattatggtg gttctgggtg gaatgggcag
     7141 catgacgggt tctatagcgg cagcgatttt tatcaccgca atgccagagt tgctgcgtcc
     7201 tcttcaagat atcaccggta tcgatctgcg catggtgatt tattctttac tattgattct
     7261 tgcgatgatt ctacgtccaa aaggactttt tggcgaatac gagatcaccg acatctggag
     7321 aaaatatgtc cgcaaatctg cttgaggtca aagctatcac catgcagttt ggcggcctca
     7381 aagccgtcga ctccttgtcc tttaacatac ccaaagggca attggccggt cttattggac
     7441 ccaatggagc cggaaaaacg accgtcttta atatgctgac aggggtctat caacccaccc
     7501 ttggagagat tcttttgaac ggccaaaacc tcgtcggtaa aaagccttat cagatctctc
     7561 atcttggaat ggctcgcacc tttcagaata ttcgcctttt taaaaatctc actgttctgg
     7621 agaatgtgtt gatcgcgact catcagcata ttacttacgg cctgcttgat tccgtcttgc
     7681 tgacccagaa gtatcaaaag caagagtctg aaatgtatga gaaaactttt aatctgctaa
     7741 agatctttca tcttgaacct aaagcacacg aaaaagccgg atctctgcct tacggcgaac
     7801 agcgcaaact ggaaatagtg cgagccatgg caacagatcc gcatttaatt ttattggatg
     7861 agccagcggc gggaatgaat cattccgaaa cccatcactt gatggaaacc atcgcaaaaa
     7921 ttcgccagga ctttaaactc accattctct taattgaaca tgacatgaaa ttggtcatgg
     7981 gaatttgtga aaatataacg gtgcttgacc atggaactaa aatcgaagaa ggaccggcta
     8041 aaaaaatcca gacttccgaa aaggtgattc aagcttattt aggagtggag tcatgaggtc
     8101 caaaatttta atgagggtgc aaaatctcga agtgtattac ggagctatcc atgcgctcaa
     8161 gggcataagc tttgaaattc aagaaggaga aattgtttct ctgattggca gcaatggcgc
     8221 agggaaaacc acgactttga gagctatctg cggactggcg gacgctcggg gcgaaatcac
     8281 cttggaatcc aaatcacttt tgaaaattcc agttcacgaa cgtgtccatc atgggttggc
     8341 tcaatcgcct gaaggacggg ggatttttca aaatcttacg gtttttgaaa acctgcaaat
     8401 gggcgcttat tcgcgcaaag ataaagccaa tattcaaaaa gactatgagc attgtttcag
     8461 cctttttcct cgtttaaaag aacgcatcca gcaaattgcc gggactcttt cgggagggga
     8521 gcaacaaatg ctcgccattt ccagagcgat catgtgcaaa cccaagttat tgcttttgga
     8581 cgagccctca ttaggtctcg cacctaagat cgtggctcaa attttcgaaa ttattaaaaa
     8641 gctcaattca gaaggaatga cgattcttct ggtggaacaa aatgcgaatc aagccttaaa
     8701 agtggctgat cgcgcctatg ttttagaaac tggaaagatc acgttaagtg ggacaggcca
     8761 agaactgctc agtaatgacg aagttcgtaa gagctatctc ggagtctagg gaatttcttt
     8821 ttgctcgata atttcccagt ctccattggt cttcttcatc tttaaaaggt aagtctttct
     8881 ttcttttgcc gccttcactt tttccttcca ctcttttcca gaaatagttt ttagagtgga
     8941 ataaagactc ttgggaaaag tttcaactgt caggcgagct gttgcttcat tctcgttctg
     9001 aagctcaacc tcgctcacat cgattcgtgt gtttaccttt ataaattcag agaagacatt
     9061 ttgttgttca gcatctacaa aattttcttt tccggcgacc tgtgcttcgt tatcaaattt
     9121 ctcaacggaa agaaataggg ccctattctt gatactttgt ttagtacagc ccaaaaatga
     9181 gaaaacaaac cccagagtga taattaaaat gatttttttc atgaggcttc atagcaaata
     9241 aaaaaatctg agtcaattgg tcataacata gtgaaaatga gactcaggct acgcaatagt
     9301 aaataagtag gagagccgac tggcctccta cttattgtct aagattatcg tttcaattga
     9361 atgacttcgt ttaagagttc atccgttgtg gtgatcgtct tggcgttggc ttggaaaccc
     9421 cgttgatttt gaatcatgtt tacgaactct gtcgccaaat caactgtgga acgttccaaa
     9481 gacttcgcga ataatttacc acggccagag gcgccaggtg cacccattga aggtcctccg
     9541 gagtccctgg attctttcaa acggttgttt cccactttga aaagagcttc gggattctca
     9601 aatttcgcaa gggcgatttg cgccaaatca gaagcctgac catttgaata aatcgcagtc
     9661 aagtttcctt catcattaaa tgacaacgac gtgatcgtac cggctgccgc accatcttgg
     9721 ttccagctaa tcaaatctga atttttacca tattgttttg ttccatcaag gcctttaccg
     9781 ccgtccttga tcgcatcgcc aaagttaaat ttaatttttt gatcttggag tgcgccgcct
     9841 ttgaaattga aggcggactt ggtcaattct tgactgtcca attttccatc caccgtgaac
     9901 gtcaacttac cttcacatac ttcagacata gcaccatctt caccaccaga aatttcttta
     9961 ccatcgacca aaccatggaa ggaccactct ctatccgctg ttttgttaaa gaacatgctg
    10021 agcaaatgct tattcccttg agagtcatac atctcgacgc ccgtagaata gtgagaagtg
    10081 gagtaaggat ctttttcgtt gaattttttt gtcgcttcca tacgagagtc caaattcaga
    10141 tccagcttga tttcggtact tcctttcgcc ggaatcaatg cacgagggaa cttaatgtcc
    10201 gccattttgt tcaggatatt tcctttttca tcggcttgga atccctgcac tcgttgattg
    10261 tcattggtta cgaggtaacc ttctctgtca aaatggaaag atccatcccg tgtaaaggac
    10321 tctccatcag atccttttac tttaaaataa ccgtctccag aaacagctaa atcggtgact
    10381 ttttcagtcg aatccacatt accttgggaa agaatgggat ttacggcacc gatcttcaca
    10441 ccacggccta tttgattacc acccagaatt ccttttaaat tttttgaaat gatatcttga
    10501 aattccgcac ggcttgcttt gaagccgatg gtgttggcat tggcgatatt gtcagagata
    10561 actcccaagg cctcaccttg agctgacata cctgacacac ccgtgtataa agacgaaaga
    10621 atacccatat gacctccatg tcattcgacg tcttcattca gaatccgtcg tatattggag
    10681 ggccccctca atgaggtccg gccctaatta ttaaattttt acatcacaat cgtactgtcg
    10741 atattcgtga agacattttc tttcaaagct gttttgtcca tcacagtcac aacagtattg
    10801 ttcttcacac tcacgatcaa agctgaatca ttcatcaaaa tcagcgagtc tttagaacct
    10861 tttgcagcgg ctttccccac cgcttcactt agccttgaga tgtcctctgg actaaatgca
    10921 attccacgcg ttctcattct ctccaccgcg tgatttgaaa acttaatccc gtcttttatc
    10981 gggatccgcg cgacttcctg tttagcgggg ccttgcaaag tacccaaacc atccagagtt
    11041 tcccgaaatg aaggcaaatc tccagttata ttacctccga ccttgcctgc ggggcctttg
    11101 gctcccgtgg gggtcgtggg tcttaaactt tcaaagcctg ttaacttctt caagtccact
    11161 tactttatct cctttgccag ctttgacatc atttcgcggg ataatcccac gttgtccatg
    11221 atgttgctct tcgccaccgc aacagaagga gatacattta tagtttctcc tgttgtggtc
    11281 ccttttttca agtcctgtgc ggtaacatct gttgctttct ggtcgttact cataaggctt
    11341 gggtcggtaa tcttacgaac gtcgcgcatt ttaattgtct gatttccgat taatagaacc
    11401 ggtccatcat tcgtataatt aatcccggag atgattccat cgaagtctgt cttaactgcg
    11461 attttctttt cccctgcttt tgcttcgata gcaaatttgt attcgccagg gttcatattt
    11521 tgacctcggt catcttgccc gttccagctg attttattct ctccttgctt tagagccttt
    11581 aagtcatagc ttctgacaac ttctccttca gcattgaaga ctttaatatt tgcctcagaa
    11641 gcatccatag gaagattgaa cttaaactca tgttctttat caccaataga gcgcgtcacc
    11701 ttggaggaat caccagccac cgctttaccg atcagattga ttgcctgata attttcttga
    11761 ggcttttgcc ccaatttcat ctcagtcaaa gtcttattca tattctgcat ttgctcaaga
    11821 cccgaaaaat tcgccagctg agcggccatt tcatgactct tcagaggatt cgtcggatct
    11881 tgattcttca tttgcgcaat catcaactta aagaatgcat ctttatcaag cttatcattc
    11941 ccgacagttc tcgttttttt cgccggatcc acgtaatttg gatccgacaa tttattaagc
    12001 acatcaccta cgctttggtc gccaaaggtc ttctgatctt tcacggatag gtttttggca
    12061 gtgttattca tttcgcttgt tgagggccct ccttggatct caccaaaagc cttcgttcct
    12121 agtttcactc ccatagttcc catgacatca cccttccttg gaggttactc aagctaccag
    12181 attgaggccc gcgccttttc ccgtcccggt gtatttattc acactggctg tttgcaccgg
    12241 ctcgagacct ttatctgttc gtcgacgagg atagcctcgc aaatttggta aatcagtgaa
    12301 gccatccctt tgttgatttg gggcaccgaa gttttgattg aacgtattcc agaattgacg
    12361 agtttgatcc cgagaactct gatgttgatt catctgattt tgcgagctgt tttcggtgtt
    12421 tgtcccactg accacatcta tcctaatatg atccatcgaa agtttgtgag ccgccaagct
    12481 atttctcaga tcagaaagac cgttttccag agtttttctg gcctcgtgag tctctgctga
    12541 catctgaaca ttcaccttgc cgtcttgaac caagactttc atattgatct tgcccatacc
    12601 ctcaggtgac atctcgactt tcatctcgcc gccgcctttt ttaatcaggt attccgcctg
    12661 attcatgatt tggcggatat tggcttcgtt ttcagctttg cttggagtcg ccatcgcttg
    12721 agctccgccc ccagcggcaa cacccattgc gatgtcttgt ttcaaatgcg gctgaatctg
    12781 aatatcttcc atgcctaagg tttttttgaa ttctgctttt tcaacattct tggttttttc
    12841 taccatctct cccttagtgc tattgccttg gggttgatgg aagccttctt cgttctgacg
    12901 gtttttcgca aattcaaaat tcttggtatc atcttgaaat gactcaccca cggcattggc
    12961 ggatgccccc ttcatctggg gcttggcctg caagggcttt accgctgcag tgggttcttc
    13021 ttcatcctca gaggcttccg cttcttccat tggaactgca tctaaggcct ggtttaaaac
    13081 ctgattcgaa gcctgattta atgctgggct tgaaacctgg ttttgtaagc cagattgagg
    13141 ggtttgattt accatttcag caaacttccc attcttcagc tcttcctcca ttggaagccc
    13201 ctgcgactgc cgtaacttct cttgcatatt caattgcgtc agcttatcta ccaagtcctc
    13261 ttttcctgct atcgtcggag agccattttc tccattcatc cagaatttct tattcatatt
    13321 atccaatgaa tcattgaggg cctgacgttt ttcctgcgcg ctgattctga cctctgactg
    13381 tacaaaagct ttgggcggcg gcgaagcctg aggcgcattc tgatttattt gcgacagttg
    13441 ggccagcaag cctacataca tctccttggc aagatcctct tgctgatcat caaggcccaa
    13501 ttgaccaatg actttgtcag cagtagcctc tggagcctcc gcttggtctg taggttgtag
    13561 gtttgccatg gcctccacga ttctcgtggg agggacaccg aattcactct cgaaagagtc
    13621 catgaagttc agtatcgcat tctgtctctg gctgctactt ttgtcttttt tttccactcc
    13681 tttatcgtat gtagccgtta ttttttccac cttctcatct gattcgggac gtcgctgttc
    13741 ttcttgttca gctccctcta ttttgttcgg agctttttct gaagccggcc tttgtaactc
    13801 cgccatttta ggcgtcatcg gctttgtctc gtctcgcctt agcacttggc caaaggaatc
    13861 agacccagct ttcttctctt catttgagtt ctccgctttc ggattctcag atgacttgaa
    13921 gtcggcgaca ccctttaatg ggggtccaac aattgattgt aacaaaatct ttcctccttc
    13981 cgtggaaaaa tattattttc ttttatatcc agcaaacttt tccgtcagta cttgtgcttt
    14041 gtccgccttc aataaactca ttatatccgc tgcatttttc tttttcatac gaccaagtat
    14101 gtctacagcc aaatcctcgt ccatggtctc tattactttg gccgcagccg gaggtttcat
    14161 gtccgagtac atttggacca aagtatcaat tttcttatcg tcacccttga ccttctcttc
    14221 gagtacgctg gaaattcccc gtcggacctc ttccaaatct ttgagccgtt tatccaattc
    14281 ttccttctga gtttgcagct cttgctccac acgtgcgagt tcctcttcgc gctcatcaag
    14341 ttcttttttt cgttcgttca gttttgcgag atgatcaatt tcatcctcgg gtaaggtttt
    14401 ttttactttc gccgctggag cgtccgcttt tgcaactgca ggagcctctg ttttgctttt
    14461 tggtttagcc ggagcttccg ccgacgcgct tcctaaaaag gagatttcca cgcgctttac
    14521 atatctctct actttgtcaa aattttgtac gcctacggca gccgcgataa gccctaagaa
    14581 agaaaccacc atcagcttcc aaggaatgga tttatttttt tttctttttt gagttcccgt
    14641 cattcccatc cgaagtttga gctgcttctc gatgtcttct tcagaaagct taaagcgaac
    14701 ttttgaattt gcaaagggat ggttagcagc tttgcgagca tttttgaaga attgatcata
    14761 tccattgttt ttcattaatt ctagtttacc gcttgcgtag attccctgca aaaaatccgt
    14821 gccagactcg accaaattct ttattggcta ttcacgctta gagaaaggct taaatcgaag
    14881 aatattttgt tcatccattt ctttttgctc atccgcttca cgttcctcac gatactcatt
    14941 aaatttcttt tctttgaatt tattgatagt tttggtctcg accgcagctt gcagcagcaa
    15001 ctcgcgcttg ccttccacca ctttctcttg ggctagaatc ttggtctttt gaagttgaat
    15061 aagaattttt tggttttttt gaaattcgtt gatttgactg agtgccggcc cctgggtgcc
    15121 accttggctt tgcaaactcc ctgaccttac gtgcgcttca tgcagagcca cttccaattc
    15181 ctggagcttt ttctgcaata tttgaagttc attcaaaaca tcatgaaaat ccttctgcgc
    15241 caagtcttcc ttgatcttcc gatgatccaa aactttttga agaggaaatt taaatttcat
    15301 gaatctttct taagattaat aattagctaa cagtccctgc atttggcgta aacactggtt
    15361 aaaattagcg cggtcttcga cgtcctgttt tagaaattga tttacgccgt caattaattt
    15421 caccgcttta tcaatccgtg gattcgaacc tgctttataa gctccgatgt tgatcaggtc
    15481 ctcgctctca cgatatgtgg ccagaatctc acgcagtttc tgtgccatct tcacatgctc
    15541 agcagagctg acgtttttca ttacccggct ggagctttgc aagatatcaa tagccgggaa
    15601 atgacctttg tgggccaaag atcgacttaa aacaatatgt ccatcgacaa tggagcgaac
    15661 ggcatcccca atgggatcat tcatatcatc cccgtcgacc agagtggtgt agaatccggt
    15721 gatgctgcct tctccttcga acgaacctgc gcgctcaagt agcttgggaa gagttgaaaa
    15781 cacactggga gtatatcctt ttgaagaagg cggctctccc gtactgagtc cgatttccct
    15841 ttgcgccatc gcaaaacggg tgatggaatc catcattaaa agtacatttt tacctttagc
    15901 tgaaaagaat tcggcgatcg ccgtcgccac aaaagctcca cgcatacgca gcagcggact
    15961 ttgatcactg gttacgcaaa caactacaga cctcttcatg ccttcggcgc ccaggtcatg
    16021 ctcaataaat tcccgcacct cacgaccccg ctcaccgatc aacgctatga cattcacgtc
    16081 agcgcttgta taacgggcca tcattcccaa aagaacagac ttaccgacac ccgaaccggc
    16141 catgatggcg atacgttgac cttgtcccgt ggttaaggtc gcgttaatgg cgcgcacccc
    16201 aatatccaat ggctcacgaa ttggatggcg ctctaaagga ttgcgtactt cattgtaaag
    16261 gcgaacttcc ttaaaatttt caaactctcc caaagaatcc agaggacggc ccaagccatc
    16321 caccactctg cctagaagtt cctctccagc ctttacagtg gcgatctgtc ttgtcaaaac
    16381 gattttagat cctaaagcca cgcctcgcat atcgcttaaa ggcatcatta aaacatgttt
    16441 atctttaaaa cccacaactt ccgctaaaaa agaacgctca agatcagacg gggtgatgga
    16501 acaaatgctg ccgacgctgg cgcctggtaa atatccttta attatcatgc cattcacttc
    16561 agtcacttta ccactgtctt tggtcaaatg aatggtgttg agaagatccg cgtatttatc
    16621 aaggttaagt tggtatgcat tcattcgagg gcacctattt agatattaga tagcaatttt
    16681 tgatttcact ttaggaacat tttccgccaa ggtctcccac agggattgaa tgcgttgctc
    16741 aatcctagca tcaacttctc catagttggt ctcgacaata cagccaccct tagcaatctc
    16801 ttcactaggc tcaaacttaa tccttttcaa gaattcgaac tgccgactat tttgcttctt
    16861 aagctcctca atgaactcat gttgctctgt tgctacccga actgttacgt cttcctcatc
    16921 ttgggcaagc gtgacagctc ctcgcaggac ttcgataaga ctctcattat ttttttcaat
    16981 ttcgaccttg gccagtttcg ttgcaatatg aaaggccaat tgcacaatat gagcttcatt
    17041 gaaattcaaa atttcggact tagccttttc tattgaattc aaaagacctt ccagttcagt
    17101 cagcctctct tcaatttccc tcgaaaattt cgtgaatgcc tcatggtggc cttcggtcgt
    17161 tccaagctta taagcttcct tataagcact ttcttgaatc tctttaagaa gctctaaggc
    17221 gcgggactcg attttttctt cttccgtggc cttttcaatt tcacttacac ctgtttgaat
    17281 gcgcagcgcc tcactcaaac gaaaatcggc gccctgcttc ttttgctcca gataatccaa
    17341 ggcctgcgct ggtgtcccga gctcgaactt cttaggaatg tactcaaaga ctttttcctg
    17401 cgccgtaatt ttaggtaaaa tagccttaga ccatggcatc ttctgaaccg cctcttgcta
    17461 tcaagatctt gccttcggcc tcgagtcttc gcgccgcatt tacgatctct tgttgtgcgc
    17521 cttccacgtc tgacaaacga gaaggtccca tattgcttaa atcctcgcgc aacatttccg
    17581 ctgcccgagc tgaaatgttt ttgaatacct tcgcacgaat gtcttcgttg gcagttttga
    17641 gagccaatag caatttttcg ttgggaatct ctttgagaag agcttgaata ccacgatcgt
    17701 cgatttttga aatatcgtcg aagacgaaca tgagcttgcg gatttcttct gctaataaag
    17761 gatccttctc ttccaagcga gacatgattg aagtctcagt gttcttgtcc atgacattga
    17821 gcatttcggc cacaggttga actccgccta atgccgactg ctctacggtg gccgtattag
    17881 ccagctgatg ctttaagact ttatcgattt caacgataag ctctggatcc acatgctcta
    17941 aattagccat ccgcaaaacc acttccgctt gaagagcttc aggaagacgt tttagaacct
    18001 cacccttttt ctccggttcc aaatgggcca agataaccgc taccgtttgg ggatgttcat
    18061 tcaccaaaaa ggttgccagt gatttcgcat cgaccagctc taaactttcc agagacctat
    18121 tgcctccggt agagatattc aagccgccga ggataccgcg agctcgctct tcacctaagg
    18181 cctctacgat cacatcctta ttagacgtct gctcagtgaa aatataatct tccgcttctg
    18241 aaaccatttc atagtactct tcaagaacac gttttgtcac atgaacagga accactctat
    18301 attttgtcat ctgattgatg agtttgcgaa tgtcaccatc atcaagcctt tttaaaagca
    18361 ctgacaaagc gtctttgccc agataattaa taagaattgc agctttatcg aatccacgca
    18421 gactttcaaa ttcaatgttt tcgcttttac tgagtctcat cgatcatcct ttctgaccaa
    18481 ccacatacta aatgcatttc cagctttctc ttcatctgta ttaagcagat tcataatacg
    18541 gtctttcagc agctcactct ccgctttttc aggatcaatg gattcctgaa gaaccggtaa
    18601 ggccgatccc attcctggta atgaattatc cactgactgc aactcttcca gctcctcaat
    18661 ggttcgcggc aacatttctt caacagaatc ttgaaagctg tccgtaatcc attgcataaa
    18721 cggccgaacc acaatgaaga aaaataatac tagagagaaa cccaacaaag cccatttgaa
    18781 taaggcatga ataaatttct tacgttcaag gctggtaata agcttttcag attcggtgaa
    18841 atcctctgcc tgaaactgga tattttcgat cttaacattg tcaccacgtg cggcattaaa
    18901 accaatggcg tttttcacta gggattcaaa tttctgaatt tcttcggcag aacgcggaat
    18961 atatttactt tcaggggttc catcttcttt agtagttgtt tcgattttgc cgtcaattac
    19021 gacagccaca ctgatacgtt ctagatttcc agcagcttct ttgatattac gaactgtttt
    19081 gggaacttcg aagttaatag ttttgatttc tttcttgatg tcttgtttaa atccgacttg
    19141 gcctgcatct tcagctccgg gaagatttgc ccgtgagcct gggactcctg ctggatttgt
    19201 ccgagccccg tcgagagatt cttcttcact ctgttgactt cgaattgcgg ttttatcagc
    19261 atctacggat tcttccactg agctgacgat ccgatgattc aaagtggcat ctaccttcgc
    19321 aatcactttc gcgtggccga ctaccttcga taaaatcgat tcaatacgtt cttgcaactc
    19381 gccttcaact ttttgtttta gatccatcaa ttcatcagat ccagcaacag tgccgtcgct
    19441 tttacgagtt aacacttttc cgcgctcatc taaaacagtt acacgatccg cgtccaatcc
    19501 ttcgaccgcg tttgcgacca aataacgaat tccccgaatt tgatccgcgc tgagctcttt
    19561 tccctgttgc aattgaagca cgacagaggc cgttggctgg cctccttctt caagaaatgt
    19621 tttcttattg ggtaaagcta agatcacttt agattgttta acggcggtta aagtgtttat
    19681 cgctctcatc aattcgccct gaagagcccg ttgaaaattt attttttgcg cgtatgaatt
    19741 gattccgaaa tcttgctttt cgaaaagttc aagaccagcg ctgcccattt tagcggagcc
    19801 tacctctgac atcaaagtca tttgggtcga gtgcaaaagt tcctttggaa ctgaaatgct
    19861 ctttccatcg tctcgcaatt gataaggaat gcttttctcg gttaacttcg ccatgatggt
    19921 ggtgatttga tcgctgggaa tattggtgaa gagaggtacg aagtctttac ccgaagccat
    19981 gaaaaccatg attatcaaag tgatgactac aatccctgtg gcagcgacta tagagagcct
    20041 tttggtaggt cccaagttct taaagaactc tctaaattga actatgatac cgccgaaaat
    20101 cttattcaag gtccgtcctc cgctatccta aaaattaaac ctgcatcttc atgatttctt
    20161 gataagcatc aataatctta ttacgcactt gcaccattac acgtaaagcg atgtccgctt
    20221 tctcggccgc aatcatcaca tcggccacat tgtcagttct gcctgttgct aaatcctgag
    20281 ctgatttatc tgagaccttc tgcaattgat tgacgttgtc gatagcggat ttcagtgtat
    20341 cagcaaaact cccttgagct tctttggact gtgacatggg ttgatcgata gatagagact
    20401 tcgaatcgcg aatcttcccg gtatctaaaa aattacttgc actggatact gtaaaacctg
    20461 ccatagtttt atctctttcc ttacttttat tctactctag aaccggtact atctgccacg
    20521 cttttcaagg agttatctca aataccgact acgtactaac gaccaatctc aagactgctc
    20581 aaagcgatat ctttggttgc ttgcattgcc gagacattag cctcgtaaga gcgagtcgcc
    20641 tgtatcatat tggtcatttc ttccatcaga ttaatgttcg gataagcgac atacccctcg
    20701 gaattcgcat ctggatgatc cggctcatac ttaagcatcg gagctttgcg atcagaaata
    20761 atatcactga cctggacccg ttggaaatct gccttcgcac tggtgggtga aacaatctcg
    20821 ccgaaacttt tcgcatcagg catcgcttca aaaaccacgt ctttgcgacg atagggcccc
    20881 ccttcgggag tttgggtcgt attgatattg gcgatgttac tggaaatggt attcatgcgc
    20941 attctttgcg ccgccattcc acttccgcta attctcattc ctgtgaaaaa atcagccatc
    21001 tacgccctcc ttttcgaaat tatctgtcac caatggcgta tttcaaagcc gccatttttt
    21061 tattaatgag ttgcagtgct gttttataca agatcgcatt ctctgagaga gcagacagtt
    21121 ctttttcaat atccactgta ttgccgtcat tattaatggc gccttctgga ttttcataga
    21181 tgtcgggtct taccgctgcg gaggccccac ctacagaaat gtgatccgca ctgctcgtgc
    21241 tcattcctcg aaggccatcg atatcaagag ctctgcccag tgccgcttca aaatccactt
    21301 tttttgcatg atagccaggc gtttccgcat tggcgatatt tgatgtggtt acactatgtc
    21361 tcagctggcg catcgccagt gaggttgcga gggcattcgt tgttttgtcg aataagttac
    21421 tcatactaaa cctcctgctc tactattgtt gcgtctgctg ttgcgacatg gccggaatac
    21481 gctgaatgta cttcttgtac tcgccacgcc actcggagtt tttcaactgt tcctgagcga
    21541 gattgtacca aaactgactt ttctgcgact tcaacttaga ccaaacatca gccgcttttt
    21601 ggacatcgcc ttttttgaaa taaatttgtc ccatcttata gcgaatcgaa gccaagggtc
    21661 ttttctcttc atattgattt aacaaactat caaaagcact aatggcctta gaaccttccc
    21721 ccttatcaag ataaagttgg cctaaaagtt ccaaaccttt ttcatgcacg gcctcagaaa
    21781 cgctcttcga atcggtcatc aacaaatcga ttttttttaa agattcaatg gccgcctccg
    21841 gctttccttg tttcacttcc agttgggcta aacggaaata gggttctgcc actaaacgag
    21901 cctggccttt ccaagttttt aagagttccg taaggtaacg aacggcggag tccagatccc
    21961 ctcttttttc gagcagatcc acagccacac gaacgcgctc gatctgctga gtttcggaaa
    22021 gcccctcagg gtttttgata ttttttaaat aatcataaga ttggttgaat ttcttttgtt
    22081 gaaaagtcgt cactgctaaa cgcagattga gttcatcttc atccggcttt ctctttgttg
    22141 cggtcaattc tttatctcta atagttcctt taattgcata cactttattg agaacatcac
    22201 gatagtgttt ttcagcctcc ggagggactc cagccatttc aaatgcttgt gcaagataga
    22261 acttcgtatc aatgcgatca ttatttttaa gccaattttc agcatactta ctatgagtct
    22321 tcagtgcctt gatgaagttt ccggcattga cctgggcgtg aatttcatca ctaatgttgc
    22381 cgacaattct agaggttaat aggttacgat ccaccacagt gggattttgc tgataatagt
    22441 ccgtcaggag atctatggcc ttctcatgct ctccgcgtga agtatatccc tctgcaatca
    22501 tgacagtccc gaattgctcg atattaggaa tatctaattt tttcatcaga gataaaatct
    22561 ctttcgtggc aacttcaact tctttaggct tcatactttt catacgtgca ctggttaaac
    22621 gaagccttgc tataatagcg ttattgtttt caccatagcg aaagaaagtt tccagatagg
    22681 cgccgacaac tcgggactta tcagcaccca gaatgtctaa aagttctccc atccgtgtca
    22741 tcgcaagagc cgcatgttcg tgagagggaa actttttcat gaattccaga taaactccca
    22801 gagcttgata atagtttccc atgccgaata atgcttccgc ttggttatag aatgcattcg
    22861 gatattcgct ttgatgctct ggaaattttt taatggcctc tttataggta tcaacggctt
    22921 tttgataatt tttggcctgg acgtagacgt caccttcccg atacgcagct tctgccctta
    22981 tttctttgag gggactttct ttttgtagtt ttgagaaaat atccgaggct tcttgataac
    23041 gattaagttt taaaaaagag attcctattc ctattctcgc cagctcattg gaaatagcgt
    23101 ctggtttgcc cgcatagttt ttctcaatgt gagtattata cagtcgaata gcatcgaggg
    23161 gatcctcttt ttcggcagcc atatttccca gcatcaaaga cgttcgctcg accaaaggcg
    23221 actgaggatg tctttgcaga gcttgtttgt attttatttg agcgatttca taatatttcg
    23281 ggtcctgttt ttgccgccac aaatttttgt aaacatcggc tgtcataaaa tcaatcaggt
    23341 cattatattc agactccgga tatttttttt gaaaccattc ttccgattta atataaaccg
    23401 catatcgctt attctcaaac aaagtcaaaa gaagacgggc ttgcttgttt tcttctgtgc
    23461 ttttggaaac aatctcataa atgggagatg ctattttcat tttttcccag taatgatttt
    23521 ccggatgaat cataggataa ggaataaaat agctctcctt gctgcgaagt agggcgtcat
    23581 ctttaatttc atagtcttta atcatgaatc gttcaaagtt aggatcgccg ccgtcaaata
    23641 agcctgagcg gctttcagct aaattagaag aatttgtccc atcggatttg atttgcagaa
    23701 tatcggttgt ggctggcttg cgagtttttt cagtttttgt tttttcagcg gtcttcgata
    23761 ccttagctgt ctttgcaggc aacacggttg tctccacggc atcatcggcg gccgtggctg
    23821 ttacggattt cactttattt tgcgcattta cataaaagtc gactatcaaa cgcgaaggct
    23881 gatccgtcaa ataatcaaaa ctctcaacat cttcgtcggc cactagaaaa ttcaaaataa
    23941 atttaccgtc gggcccttct ttttccaagg ctaccgcctt caccatttca ctcttaaagg
    24001 ccaagagttt gcggatcgaa gcctcgtcta tggcatcgac agtcagctgc aaatagcttt
    24061 tatctgactt cttaactcga cgaaggtcat aatcccaatt ttgctgtcca gaaagttcca
    24121 aatggaccgt gtccccttgg tctgagatcg agctagagac cctcgcggcc cataccacag
    24181 aggcagaggc aaagactaga ctcatgagta agctgacaac gataaaactc aatttcatat
    24241 ccattgtaca ttgcacatag agtgccaggc aaaaatgagc tctatagcaa atattttcca
    24301 gttgggaaaa aataggccat tgatttttgt cggtgtccga caaactcatg actcttccaa
    24361 agtctaggtc cgccagcagc tatgttaaag gtcgagaaat acttataagt atcgattaag
    24421 tgctgaggat ttttaatttt ttaccgatga agggattatg aaaaaccacg tccttcgtta
    24481 tcttgtacta actggatgcg tgcttgagtg tctcctaggc attggagcct gcgtttcagt
    24541 gaagctaccg tcgagcatga gaacgcctgc aaaagatgtt caattccaag tacctgagaa
    24601 accttttcaa gaaatcaaag ttctcactgc tgacaaagca tggctcagtg ccgagactgg
    24661 aaatacaatt tcttttctct cagaatgcaa tggaaacgta gaccccaccc tggaacagtt
    24721 gcaaaatgaa tctctgactg ttctggataa acttaaaatt ttacaagcaa aaaatttgga
    24781 atttaatggc cgcgaagcac tgcgcacgga agctcaaggt gaagtcgatg gtgtacccgt
    24841 aaaaatggaa cttctcatat ttaaaaagaa taattgtaac tacactttga gttttggcgg
    24901 cgttcttaaa aggtttaata ctgagaagaa atattttgat aaattcactg aaagctttaa
    24961 ggcgccctaa tggatgcact ccctgttgcc acactcttag ttgaaatcat ccgtttctgg
    25021 ggcggtctgg gtattttgac cggccaagtt ctgcgcgaaa ctttcaaagg aaaaatttac
    25081 tggaaactgg ttcttgaaca gatttatcaa gtgggttaca ggtcgctgcc cctgattctt
    25141 atcacttctg gcagtatcgg catggtcatg gctctccagt ttggattggg tcttgaaaaa
    25201 ttcggcggca agctctatgt tccaaaactg gttgcggcaa ccatcctgcg ggaaattggc
    25261 cccatgttca ccagccttat gttcgctgct cgtatcggag caggtatggc cagtgaaatt
    25321 ggctctatga tggtgactca acaaattgat gccatccgtg ccctcggcac ctccccaatt
    25381 aaaaaaattg ttattccacg agtgcttgcg tgtttattcg ccttgcccgt actggtggcc
    25441 tttgcaaata tcattggaaa tttaggcagc cttttggtgg gcgccacaga attgaatctt
    25501 gacccccatt tcttttacct caaaatcatg tcgaccgcga gcattgacga ctacctctcg
    25561 ggatttggca aaacattctt ttttgctatt ttcgtttcgc tgccatcctg ttactttggt
    25621 ttgaatgtca aaaatggagc taaagaagtc gggatcgcaa cgactaaggc tgtggtggtc
    25681 tcatccatat tgattctagt cggtgacttc ttcctctcaa aagtattttg gatgattgca
    25741 aaatgggtgt aatagacgtt atccacttta aaaaatcttt tggaaaaaaa gtcatccatc
    25801 aagatgtcac cttcagcgtt cagaagggag aatgtctagg actcattggc ggttcgggaa
    25861 ccggaaagag tgtgatttta cgcagcctca ttgggcttga aaaacctgac agtggccgag
    25921 tcattattga tggaattgac atcacccaaa tgactgaacc gcaactcctc gagatccgaa
    25981 aaaaagtcgc atacgccttt caaaacggtg cactttttga ttctatgact gtgtttgaaa
    26041 atcttgctta tcctctccgc gaacatttcc attatagcga aaaagaaatt cacgaacgtg
    26101 tactttccag tcttgctgag tttggccttg aaggaaatga aaatatttac cctggaagtc
    26161 tctcaggcgg catgcaaaaa cgcgtgggct tggtgcgtgc catgatgatg aacccgcaga
    26221 ttatcctcta tgatgaaccc acagcgggct tggatccgtt taacactcgg aaaatccagg
    26281 atttgatctt gcggttaaaa gaaaaaggcg tgacttccat tctcgttact catgacatgc
    26341 cgaccgcgga cgctgtctgc gataagatgg ctatgatttt aaatggccga attgaagctc
    26401 aaggaacctt gtctcaactg gaccaggata gcttaggagt cattactaag tttatcgctg
    26461 gcgaggaact ctaggaggat tgatggaaaa caatttaaaa actcaatttc gtgtaggact
    26521 ctttctttcc atcggcctga ttctgatctt aggctccatc ttcatgctgg gaggagaccg
    26581 ggcatttttt aaacgataca ccaccttgca cgctcacttt gaacaagtcc aaggtttagc
    26641 ggaaggttcg atggtttcat tttcaggaat caccattggc aacgttaaaa aaattcaaat
    26701 tttgcctgaa agaaacctga tcgatttagt gttaaaaatt gataatgaat tcatgccgcg
    26761 tgtgactgaa ggctctcagg tggagattcg tacccaagga gctttgggag ataaatttgt
    26821 atttattatt cctggcgatc caaaagcgaa gcctcttgcg gatggaagca ccattgaggt
    26881 tgccaaggcg acggacatct tggggattct ctctgagcgc ggcaacgagg ctgaaaaaat
    26941 cttcgatatc atcaacgaga tgtaccattt ctctaagaca ctcaatgctg aaaaccgcac
    27001 ggataaaatt ttatccagcc tggcttccgc cactacgaaa atggataaga ccgctacggc
    27061 gactcagaag atcgctgaaa gcttcagtga tggaaaatta aaatcgacac tggatcattt
    27121 ggaaagcgtg attaccaaat tggatcgcgg ccaaggcact ctgggtgcct tgatcaatga
    27181 cccctccgtt cacgaacaga ttaaatccat gttaggtggc tccaaccgta agagctatat
    27241 aaaatctttg atgcgcactt caattgagaa gaccgagcaa aaatagttta gttaagatat
    27301 cggcaaggct attccttgtc ttcattttcc ggagctattt tttgaatcac atttctaaag
    27361 ttattttggg attcattatc gctaggaact tcccgccgat cgagagtgaa gcgaatattc
    27421 ttaatcaccg gtttgcccaa ttttttattt aaagtctcct gaatgtgatc cttcatgaaa
    27481 gaaagttgat gcatccacgg actgctgcga acccaaagat agagaacgcc gcgattaaaa
    27541 cccacaggct ctgtgttttg cgcgattgtc ggccccgcaa aatcccccca atgagcccat
    27601 agcttccaac gcataaattg ttcggataat ggcgaattac caccttcaaa caacttctga
    27661 aggacctcag agccgaggct aaactttgtt ttaggatctc tttcacgcat ttcttacact
    27721 tagcacagcc ccggattgaa atcctgtgaa aaaactctag agatggcctt atcactcctt
    27781 gtcaatggag ctaagtaccg ataaacaggt cccatgagtt tgaaaacaga aaaaaaaatc
    27841 acagttgtgg gtgccggact tgctggaagc gaatgcgctt tgcagttagc tgaaatgggt
    27901 tatcaagtgg ttctttttga aatgcgcgat aaaactatga ccccggccca caaaaccggt
    27961 aagttcgcgg aactggtttg ctcaaactcc tttggcagca tgggcgaggt ttcggctccc
    28021 gggcaattga aatgggaagc gcaaaaatta aactcaaaga ttcttgaggt cgcgcgcttt
    28081 gctcaagttc ccgcaggcca ggctctgggc attgatcgtg aagcgttctc gtccaaagtg
    28141 acagagatga tccgcaatca tccgaatatt gaagtacgca atgaagtgat caataatctt
    28201 gaagaggttc cccgtcccgc agtcatcgcc accggccctt tgactcatcc tcctctggcg
    28261 gaaagcatgt gcagtcattt tggtaatgag ttcatgtact tcttcgacgc cattgctccg
    28321 atcattgata cagagagcat caacaccgag attgcttgga aagccgatcg ttatgacaaa
    28381 ggagaaggag gcggggacta ctttaactgt cctctcaata agcaagaata cgaacgcttt
    28441 atcgccgaaa taaaagcagc acgaaaaatt gaacctaagg attttgaaac cactgctttt
    28501 tttgagggct gcatgcctat cgaaaccatg gtggaacgtg gagatcagac tctgcgtttt
    28561 ggaccgatga aacccgtagg tcttgataat ccaaaaaccg gtcgctacgc ttatgccatt
    28621 gtgcaacttc gtcaagacaa caaagaaggc accgcttaca atatggtggg ctttcaaact
    28681 cgcatggctt acagtgaaca aatccgtgtc ttccgcatga ttccaggttt ggaaaacgcg
    28741 gagttcttaa agctcggcag catccatcgt aatttatata ttaactcacc caagagcctc
    28801 aattgtgatc tttccagcaa gcgcgatccg tggctgtttt ttgcgggcca aattacaggt
    28861 gtggaaggtt attttgaatc cacttgcgta ggtcttttgg tcgcaaaatt tctggatcaa
    28921 aagcttcgag accaggtttt taatccgccg ccacgggctt ccgcgatggg atctttacta
    28981 gaagccatca ccgatcctat acgagcgtcc catttccagc caacaaacat aaactttgcg
    29041 ttgattccac ctttaacgga agttgagatt gaagaactca aactccaaaa gcttcatcgg
    29101 gataaaaaag ctaaaaaaga aatacagatc aaaaaagcac agacggccct tgttgaatgg
    29161 ctcggataat atctagaatt aggacggcgt ttcttgtctt gcttcgtccc atggggtaac
    29221 ctaagccatt atttttccaa ggataataag gaatggttat gtcagaaaaa tcccaaagcc
    29281 agaatgcact ctccgatcgc tacaatcctc aagaagtcga aggccgggtt tataaatggt
    29341 gggaggaagg cggctacttc aaagccgaaa atcgctccac caaacctccc ttcagtatta
    29401 ttcttccgcc tcccaatgtg acagggttcc tgcatatggg gcatgccctg gatcatacaa
    29461 tccaagacct tctcattcgc tggaaacgta tgaatggatt caatgccatg tggttgcctg
    29521 gaacagacca tgccggtatc gctacccaaa gtgtcgttga gagagagctt aaaaaaacag
    29581 agcatgtgac tcgccatcaa ctgggacgtg aaaaattcgt tgaaaaaatc tgggattgga
    29641 aaaatcaata tggggatcga atttacagcc aaatgcgccg cctaggggat tcctgtgatt
    29701 gggaacgggc gacttttact ttggatgaag gtgtttccaa atctgttcgc aaagtttttg
    29761 tttcccttta taaaaaaaat tggatctata aaggccaacg gctcatcaac tggtcaggcc
    29821 ctctggaaac cgcaatctca gatcttgaag tggagtacaa acaagtcaaa ggctctttgt
    29881 ggcatatcaa ctatccactg gaggatggtt ctggattcct cacggtggcc accactcgcc
    29941 ctgaaactct tttgggagac tccgcggttt gtgtgaatcc tgaggatgat cgctataagc
    30001 acttgattgg taaaaatgtg atagttcctt tgatcaatcg caacgtgaaa atcatcgctg
    30061 atacttatgt tgatgagact tttggttccg gcgtggtgaa gataactccc gcccatgact
    30121 tcaacgatta taagatcggc aaaactcaca atctggattt catcaatatc cttactaaga
    30181 aaacgacact gaacgaaaat gccggccctt atcaagggct caccgtcatc gaagctcgca
    30241 aaaaagtggt cgaggatttg aaaaatcaga accttctggt caaagaagag ccccatgtga
    30301 ataacgtggg tcactgctcg cgttcgggcg ctgtggtaga gccattcctc tctgaacagt
    30361 ggtttgtaaa gactgagggg ctagcggttc ctgcccgtcg cgtggcagag agcggaacta
    30421 ttcgcttcga acctgaatcc tggacaaagg tttatcttca ctggatgaat atcatcgaag
    30481 actggtgtat ttcacgccaa ctttggtggg ggcatcgcat tcccgcgtgg aactgtgcgg
    30541 attgcggtca cgtcaccgtt gcggaaacag accccactga gtgcgaaaag tgccttaaga
    30601 aaaatctttc tcaagaagaa gatgtgcttg atacctggtt tagttcggct ctttggcctt
    30661 tctcgaccat gggatggcct gaggaaaccg aaaccctgaa aactttttat cccacgaact
    30721 atttggttac gggtcatgat attattttct tctgggtggc ccgcatgatt atgatgggtc
    30781 ttgagttcaa gcgagatgtg ccttttagaa ctgtttatat tcacggtcta gttcgcgact
    30841 ctcaaggcaa gaaaatgtcg aagagcgctg gcaactcggt cgatcccgtg gagatgattg
    30901 ataagcacgg agcggatgcg cttcgctttt ctttcctggc ccatttgtat tctggcaaag
    30961 acttcaaatt cgcagagcag cgccttgagg gctatcgtaa cttcatgaat aaaatttgga
    31021 atgccgccag atttgccttg agccatttgc aggatttcca agtacctgaa gaaggggtaa
    31081 aagcacttcc caataaatta cagatctcag tcttcgacca atggatcatt actaaacttg
    31141 gagaggtgga gaaaacggtt gaagaagctt tggaacaaga gcgcttctct gatgccgcca
    31201 cggctttgta tcaattcacc tggaatcagt tctgcgattg gtacattgaa ttcaccaaac
    31261 ctgttttctc aggctctaac aaagaggagc gtgcggcaac ccagttagtc atggctcagg
    31321 ttttaaatcg tatgatgaga ctcctgcatc cttttgctcc cttcatcagt gaagagattt
    31381 accaaaagct tccgattcgt ggcaaagctt gcgtcgtgga ccaatatccg aaccttcgaa
    31441 acgacaagga cttccttgcg ctggggtccc cgcaagcggc ttatgagatt gatattgtca
    31501 aagacgtggt gacagcaatc cgcaatatcc gcggcgaaaa ccgtatcagt cccgctgaga
    31561 aactaaatat ccgtcttggc atcgaagaag cagagatgca aaaaatcctg ggtaataacc
    31621 gaaatgcaat tttgacgatg gctcgtttag gtaacttgga aattggtgag gacggcaacc
    31681 tgcaaaaatg cgcggtggct ccgatcacta ccaagagagg tcaaatcaag gttattattc
    31741 cgttagaagg actcgtcgat ttcgatgaag aaatcaaacg catcaataag accatcgaaa
    31801 agctgggcaa agatatttct ctgctcacca acaagctttc aaatgagaag tttatagcca
    31861 acgccgatga ggacgtcgtc gccgcggaca aggaacttct ggtgcaaagc aaattacaaa
    31921 tcgaatccat gcgagcggct ttgattcgat ttcaataact gaaggaagca attctttcag
    31981 ctattgggct cccttcattt cccgtgacga aggcttatat ttattggaca aggacgtgtc
    32041 aggaactgga ttttttagaa ttccccaatg actcagaaca ctatcgaact ctgactgact
    32101 aattgatttt attgttccat tcatatccat aatattaccg cctaataaag ttcgcttaaa
    32161 cggattttta ctaattttga aagataaatc taatagcaaa cagggtgttt tactcgttaa
    32221 aatcccattg tttctgctaa ctccatagta agatccagac ttgaccctta ttatctcacg
    32281 cttgtaagca tccattgctt tgaggttaca gccctcctta tctttaatgg gggcattgat
    32341 tttctcttct gatttggact gggactctcc attaacaaga gacttaccat caacattagt
    32401 tgaggagagt tttgcaatac tcaggttttg atcagcaaaa attccgttgg gatccgtaag
    32461 caattttaac aacggatcgt cagactctaa tccgtcttct cgaatatctg ccaggctttt
    32521 tagggtgaaa ccccagcccg agccctcatt cgcaggaaat ttcacccgtt ctataagttt
    32581 tgaaaactca ttagggtaag cttgtttata gagctttaga ttttgagtcg ctgttgaaaa
    32641 atctttttcc gctgctttta gatcagattt aattaagagg gttttcttcg agacactatc
    32701 aagtgcttta tattttcgat aggattgata ataggtgtgc aacattcgcg aaaaataagc
    32761 atctttcagt gaaatgggtc ctccgtccga aatgggagtc caaacttcag aactttcagg
    32821 aaggattgtc gtagaagtaa accctatttt ttcaactttc ttgctaaaat cttgaaatgt
    32881 agttgagcct ctaaaagtcg aagtcagctg ggagtatgaa ttgttttctg gccaagatat
    32941 attttgatca gcgaaaatat actgagtaaa tagcattaca aaaacgaaca atattcgatt
    33001 attcacgaag taatcctttg gaataaggga catccagtcc tggttactaa cattacatac
    33061 cagtgtattt agtttttcaa cgcccaagtc ctacagctta agtctagaag ataaagcatt
    33121 tttccttggc gccgcccggg ttctgttaat ttagctcaac tcgaccaaaa aaactggtcc
    33181 attgctaaac ttcatcagga agacgacgtc ttcgcagcct cggcaataat ctattctatt
    33241 cactcattcc acctcagtgg catcattcag aagcggattt agaacgtatg atgccaggaa
    33301 gcgcgaagga atggttttta tacggctatg ctccctggga ttttccgcac ccggctaaaa
    33361 aaataagtag cgctaatttt agctatttat agtacccgat cgtggtccta aatcgatatt
    33421 ttgtaataag aagaccacga tcgtggtcta taatgtatct ttaatattga atagaccacg
    33481 ttaggggtct attctattca aaatgaaaaa gaaaagaact gttccgtcca aaaggcgcac
    33541 taaagaagaa ttaaagtctg atcttcccac aatctcgctt ttcgtgcgaa atcgccgcga
    33601 agaattgggt tacacccaag aagaactcgc cttccgtgcc gggttgagcg tgcgatttct
    33661 taaagaattt gaacgaggga agaaaaccgt ccgtttagac aaagtcattg aattgttgga
    33721 attcttgggt gccaccctag aagtaaaggc tcggagtcgt gaatgaaccc taagggaaag
    33781 gtctatttta aaaatcagct ttctggagtc attgaaaaaa atgaggacgg atctttttta
    33841 ttttcttacg aatcccactc tccttccgta agtttaacat tgccaacctc taagactcac
    33901 taccagtcag aagaactgtt tccttttttt gacggcctca tcccggaggg ttggctttta
    33961 aacttggttc agaaaaactg gaagattcca aaaaatgacc gtatggccct cctgcttacc
    34021 acctgccagg attgcatagg cgccacccat gtcctcggcg agcaggacaa caccaccctg
    34081 tcgaagtctc acgctaaaac atctctgaat caccgcgaag ctcatacctt tacctcggat
    34141 cggtgtttag tatgtatgga agcactaaac tcgtctgagc aactctatca tagcaaatgc
    34201 gcaaagaatc tctttggctt ttcccatgcg ccaattattg atgttgtctc tgaggatcta
    34261 gaagaactcg ctttgaggca aatcaaccag cggctgacct tgaccggagt gcaaagaaaa
    34321 ttatcactgt ctccaataat agaaaataaa aaagagcgtt tgaccgttgt aggtgtggat
    34381 ggccggttta ttttaaaacc tgcatctgaa gactatccag aaatggccga ggtggaacat
    34441 ctctctatga agatagctga attacaaaac atcaccgtag ctgattgtgg tttggtttac
    34501 ttagccagcg gtgagcgcgc ttatattacg cggcgctttg atcgtgcaaa taacgaaaaa
    34561 attcaagtgg aggatttctg tcaacttgcc gaaaaaccca ctgaaaggaa atacagcgga
    34621 tccagcgaaa gtttggcgaa gatcattgaa aaatattccg atcaagcccc ggatgataaa
    34681 ctcactcttt ttcaaattat tttattttca ttttggatcg gaaattcaga tatgcatctt
    34741 aagaatttct ctttgtggcg ggatccacaa agtggcctca ttcgcatgag tcctggctac
    34801 gatttcctaa gcacacgcct gttgatcact gccaaagaag acaacgagga gctggccttg
    34861 cctgtgaacg gtaaaaaaaa taaactgcga tggtcggact tcgtcgctct tgccagcaat
    34921 ttcaaaatcc cacctaaagt cttcatgcga attcgtgatc aaatgctaga ctccttcttt
    34981 gatgctgaag acctcgttga aaaaagcttt ttgtcatatc aaaagaaaga gaagtttatg
    35041 gacctcctcc gtgaacgctc cgagcgactc gctcaaaagt gaccaaagag tggccatctg
    35101 actgacattc tccgtcactt ttctctctat aaaaaattca aagaatgctc cgtgacgccc
    35161 tggattcgat ctcgtggttt ggcactctca ttgcttcttg ctgtggcttg aagaacgtaa
    35221 cggaggatta aaaatggaat tctttttatc actaggtcgt gcatttacat catcagacgc
    35281 tatatggatg tgggctattc tgtccgcaca gatcgtctct gtggctatca tcatcgaaag
    35341 agccattgct ctttttgtga aacggaagga caaccaaaag tctctatcta aattaatcgc
    35401 tgaagacatt cgcgctggta atctcgacaa ggctctccgc agatctcagc aaatcggcag
    35461 caaagatcca ctcggtgtgg ttgccgccgc tggaattcaa gctgctattg atatgggcgg
    35521 caaagaggaa attcagttaa aaatggatga ggtgcttctt gaagaaacga cgcgtgttga
    35581 aaaacgcatt ggcttcctcg cgatgtttgc caacgtcgcc accttgatgg gactcctcgg
    35641 aactatcaca ggtcttatta actccttcgc tggtattgcc aatgcgaatg cggcggaaaa
    35701 agccagcatc ctttcaaaag gtatttcgct ggcgatgaac acaacggcct atggcttatt
    35761 ggtcgccgtt cccgctttga tcatgtatgc agttttgcaa aatcgcgcat ctcgtttaac
    35821 cgatgatttg aacaaagcag ccttgaatct ttttattcaa ctgggatttc actactctcc
    35881 agttacggat aagaagtcta aaggtaacta acgctaactt gcttagtcta accagatgtt
    35941 ttaacaaggc ttggagctgc gtatgaaaaa ggaacttaat ttcgaactca atattctgcc
    36001 ggtgttggat attctctcgg ttctgatttg cttcctcttg ctgacggcgg tgtggataca
    36061 actcggaacg attgatacta aacaggctat cggtgacaac tcgaccgccg gggctaagaa
    36121 tcctccttcg ctgtgggtga ccctcggcaa agatgggacc gtgctactct ctttacgtga
    36181 tttacctcag gctaaaaact tagaagaaag cattgctaag attgggagtg gagtaaactg
    36241 gacccttttg gaagagaaga ttcaagatct acgaaacaaa tggccagaac tcaaaaccag
    36301 tattgtacgc cctgaagccc gggtctctta cggcgatgtg atccatatta tggatcaatt
    36361 gaagaaaaac aaattcgatg gagtgggttt gtcccctctt gggtaggctc ttatgaaaac
    36421 agctgctttt atctcacaac gacgctctcc ccttttacaa gccctgtctt tgaaagcggg
    36481 agagaaatcc gcaagccata ccaaagaact cggtttggca ttgcccttga cctcactgat
    36541 tgatgctttt tgtatcatcg ttatctactt gttgatagga acacagacct caggtgtgga
    36601 aagccagctg ccaggaaaaa tcaatcttcc gaatgcggaa cacggacaaa ctttggaaaa
    36661 gcctgtggcg acactgagga tagaaaaagg aaattacttc ctgaatgaga aacctgtgga
    36721 tcgcgcctct ttaagtgcca aactctccca tttaaaaaag gaaagtacag aagctacgga
    36781 gctgatggtt caagcagata ctcaaatgga gtatgcggaa cttgatccac tcttaaaggc
    36841 cggctctgag gccggtatcg aaaaacttcg ttttgcggtg atcccttcaa aatgaaaact
    36901 ttaaaacact tccttatttt tagtgccatc ttcggttgga ccctcctcgc agggatctcc
    36961 attgcttatg cagagaagat gaatactcaa actcaggata tggttattga gagaatggaa
    37021 cggattgttt cggcaatgga aaaatccgat aaatcttggt tgccaagcca gcaacgcctc
    37081 gctgatttat tatcagaaag agcccgcctt cgtttcatgc aggaagtgga ggcgaattgc
    37141 gatgcttgca aaggctctaa agccgaccgt cttaaggccg tttccatcta tgaaaacctt
    37201 ctacaagaag taaaagtgaa tgatcatgga cccattctgt tccagttggc tcatttgcat
    37261 gagatggctg gccagccgga tgcagccatt gccttgtttg aacggattat caaagaagcg
    37321 aaagctaaaa aaatctctaa ggatattgtc agccattctc aagcaagcct tggagatctg
    37381 cttttccaaa aaggcaaatt caaagaagct tatacacatt ataaaatctc tcttaaagac
    37441 acgaatctca aaggccgagg gctggtcatt tacaatatgg cctggtgcga attcaattta
    37501 gataaattga attccggcat caccacaatg gaaggtctgc tcaaacgtcc agaccttatt
    37561 gtgcgtgaaa attcggatgg ctctggcgaa gccaatggta caaaatacga tcccgtattt
    37621 catgccgata tcgtcagaga cttggcgacc ttctatgctc gccgtccgat tactaataag
    37681 gaaatcacaa gctacgaaac ttttgccccc aaagaaaagc gtaaagaatt attattgctt
    37741 tttgcttcgg aagcggatcg tcttggacaa aagaaagccg cagctgcgat ttataatcat
    37801 tacttagaag atacgactct ttcacaagag gaacgcttag aggcttttgt caaattggcc
    37861 caagtaaact atgacggtgg agatgctacc aaatcgactc aagattttgc aaaagcagct
    37921 ttggaattcc aaaaaaattg caaagaagct gaaaaatgcc aagaacttgg gaaaacgatg
    37981 aaacactatg taaccgaaat tcatcgagcc aagaaattaa aaccggatgc ggatttactg
    38041 aattcgtatc ttatttataa taagactttt cccaacgaca ccgaaatggc tcaattggga
    38101 gcgcaggttg ccgacgacat gggtaaacac gccattgcga tgcagttctt gcgcacagtc
    38161 agcgacagaa aatccgctct tcctcaaagc cagcaaacag cgctcgtcag cgaactttca
    38221 gtggccgaga aatctaaaaa tccagttctg caaaaggcag cttatgagca ttatctagct
    38281 gtatctccgc aaggaaataa aagctttgaa gttcgctatc aaatggccta catgacttat
    38341 cagcagaaaa attatcgcga agcggctcaa gcctttaata ctctagccat ggataaatct
    38401 ggttctgccg aacttcgaaa gaaatcagcg gatctttcct tggattgcct cgttcaaatg
    38461 aaatacgagg acagccttga agattgggcc tggcagtatt cgacagcttt tcctcaagcc
    38521 aaagccgagt atgaaacaat ggcacgtaaa gctttgttaa accgtgtggc acgcattgcc
    38581 aacgataaga actccagcaa tggtgaatta aaaaaagctt tgcaacaaat gcaaagcgcc
    38641 aatatggcct cagcaaaacc tgctgataaa gtcattttct atactaattt atcggttcta
    38701 gcgcagcgac ttggcgaaga gggcatttta atcaactcgt tgcagacctt ggtctctctt
    38761 cctggagtgt ccgcggatcg gcgagaagaa agcttaggac aaattgtcag ctattatgaa
    38821 aagaaattgg actttaaaaa cgcctaccat acggctctac gaatgaagtt ttctaaaatc
    38881 agtctcaaag acaaagagct tcgtctagga accttggcgg atttagccac tttaaaaccg
    38941 caaaaacatt atcacgcttc cttaaatgcc ggacttaaag gagaaagtgc gcgctctgtg
    39001 cgttcacgtt tggtcttatt gtcaacaaat cctaccagag aattaaaagc tcaagctgat
    39061 gagttagcaa aggatccagg tctattaaac gaaataacac ttttagtcta tgctcgcacc
    39121 ggagacggta aagcattgaa gccggtttta gctaggcgcg aactccattc tcgaaccgct
    39181 ccaaacttca ttgataagca aggtttttat aaggaagttg cttcctttaa agagcagatt
    39241 tcgcgtcatc aactcagctc aaggtctgac tctagtctgc aaagaaccat caaagagcgg
    39301 gttaaacttc taaaatcaac tgataccttc ttggcaaaca gcttaaaatt taaagacgtg
    39361 acggctcagt tgatggttct gaatatgatc tccactgaga atgaacgtat ggttcgtgat
    39421 cttgtggcct tgcctatccc gccaaaactc acggcttcag aacagaaaca atacctggat
    39481 attttgaaac taaaatcaaa accttacttt atgaaggcca aagtcgctca acagagagaa
    39541 tcggaagtct gggaccattc tccagcaatt ccccaattac tcaaagatta tgaaagtgtc
    39601 cgaccagagc tcaaaaagta tctgcatcgc gaactaacgc tcttagcggc cttatcgaag
    39661 gataaatccg gctcagttaa taatggcaaa atgcgttcgc gcatcgtgga tgccctcaat
    39721 gagaactcct cttcaacatg gagtaaccaa tggagcgatc tggtaaatgc caggaaaaac
    39781 gtagcggaaa atcctgagag tatgagcgat attgaaaaat tgaaaaacct agagacaaaa
    39841 atcggtcatc ctctgatgcc gtcttacctg gaagctcgtc tcagtcgatt acagagaggc
    39901 aaaaccctat gaaatttaca ataactttgg cctctttatt gctcattcta tcgacagctc
    39961 taacggttca cgcaggcact gcgacaatgg ttaagaagca tagtaaaaag aattcgcaag
    40021 cgaagttagg aacttccttc aagttcgatg gttcgaatct gcgtggtaag tatcagaatt
    40081 ccctgagcac gaccgcgaca gtagagaatg ataaattttt agaagatctt ttgggcggaa
    40141 gaactcagtt tgaagaccga gatcaaaaag aacgagaacg aaactaagga tgttggacca
    40201 tgagcactgc aagaaagttc acacaactgc tgatacttga gaacacgttg ggacaaaagg
    40261 ttcgaacctt tgctgtggat tcgcgttccc tgaatatcgt ctatctcaaa gatcaacgcc
    40321 gcattgatgc ggtggcaaac ttgaagaccc ttgaggacag ggacctcgaa tatattctct
    40381 taaagacttt cgaagttaaa gacctagata gtaaggcttt aaaacttcaa ggcttaggtc
    40441 agttgcgaat gatggacgtc aatgcggttg atagccctga ccatcaattg gctccggaaa
    40501 aggataaaaa agaactcaag gttattcttg aaaagacttt catcagccat atccttttag
    40561 ttcttatctt gatgatggga gcttggattt ccacaaagtt cttctcacag aaaaaagaag
    40621 agccagcttt agtgaccatc gtcatgacaa agcctgagcc taaaatcgaa aaagcgaccc
    40681 cggctcccca tgtgaaagtt tctgagaaga aaattaaacc cactcctaaa gtctatcgcc
    40741 ctgtagttca aaagaaactt ttgacgaagc cttataaggt gaaaacagcc aaggcgacgg
    40801 atgtccaacg tgtgggagct ttggccgctt taggcggtct tgcaaaaggt catcgcggag
    40861 ctgagggcct tgatatgaat tcccttaaaa atatccgttc tgcaggtgaa ggccaaggcg
    40921 gtggtggcgt cggcgccaca ggtcgcggtg gcgtaaaagg aatgatgcca ggaagtggcc
    40981 tcattgccgg cagtgccgga gagggcggac gcgcagaaag cgccggtggt tacggcactc
    41041 gcggtgtcgg cggcggccgc gctggttatg gaaaacatgc catcgtaggg ggaatgaccc
    41101 ccgtgagttt accacttgat aatgaagcca ccgttgaagg cggcttggat cgtgatcaaa
    41161 tcatcgcggt catcaataaa aataaaggtc aaattatcta ctgctatgaa aaaggtcttc
    41221 aagctcaacc ttccattggg ggccgtgtcg cggttgattt cacgattggt ccttctggaa
    41281 gaatcacttt agcaaaagtg gcccagactt ctttaggttc taaattcgta gagaattgca
    41341 tgcttgataa aatgagaacc tggcagttcc ctaaacccgt tggaaaagtc aatgtcgacg
    41401 tactctaccc gtttgaactc acgcgggtaa gtagccgata aggagtattt atgaataact
    41461 ttttgcgatc ctcattatta tccgcccttc ttttaaccac tgcctgcggc ggcagctcag
    41521 acaataagtc ttttgaagat atttctgaat tgcgcgcccg cgccaaaaaa gaaatgacta
    41581 caggcccgca accgccgcct gaaagagtcg tttacgtcac aaaagaaaaa gaagtgatta
    41641 aagagcaggc gacgctaact gatagttact ttaaaatcga cctttctgaa aaaatgatga
    41701 gtttttatga aggccagcct tcggcctaca aaatcactct tcgggttcaa gatccccaac
    41761 tacaaatgaa actgactgcg aaaggcttgc cttcaggagc agagctcaaa gatatctcaa
    41821 cggcgaaaaa tcctaacact tatgaacttc gttggacacc gccgctttac accatttctt
    41881 atgaccagca accacctcaa attatgacag caactttagt tccggtgcta gtatccgcta
    41941 aaacatcgaa aaaagcggaa acagtgaaag gtctttcgct cgacagaact atcaccttta
    42001 acgtttttag aaatcaggat aagccttctg agttggttct cagtggcctt gatcaggatg
    42061 tgcaggaagg acaagtagtg cctttctcgg tcattgctcg atttcctggt gtggatacca
    42121 atgctcctat aaagccagat cttggattct ttagagataa gaacacccaa gtcgtcgcct
    42181 ccaactatct agaaatggat ggcactcgat atgtctctat tgagaatgtg gagtatattg
    42241 gtgattacaa atggaaattc aatcttgttt tcgataccaa aaataaccct gttgaaactc
    42301 aaaaatcaaa agatggcagt gctgcggcag tgaacttcac tcaagttcgc ttagggctaa
    42361 gagtgttcgg tgccttcaat gcgagtcccg caacagtggt gcgagttaaa attatccgcg
    42421 ccgctacgga agcaactcct gtggcccccg ctcctgcacc agctccggct ccgcctaaag
    42481 atcctgctcc ctctaaaccg actccgaaga aagctcccaa gcccaaaagt cctactaagc
    42541 caaaatctgg aggtaaataa ttatgaaaaa cttgaaagct tccactcttg ggactcttct
    42601 tttaatttct ttgagtgttt cttggaatgc ctcagctgct tcaaaggcta ctccaacttc
    42661 gccagcgact ccggcggcac aagcgaaaga aataaatttt tccaaagacg ttgatggact
    42721 gggtggcaac gacgctttga tggaaatggc tgaaaagctc aatcctgaat caaagtctcg
    42781 tatcgttcaa aaccgccttg tagatcgcca cactcgtttg gaagctggca ttttctacgg
    42841 tggtgttttt ggtggagcca cctatcttca aacccaaagc tatggggcca ctcttgattt
    42901 tcacataacc cctcgctggt ccttgggcgc acgatattat cagtatcaga acaaacttac
    42961 tccagaaggt gaacgcgcct ttgatgaagc ccgtgccaat tatgctgctg gcagccgcgc
    43021 aactctcgtc gacattgatt ctccactgaa tgcgaaaata gcggtgatca actggtaccc
    43081 tatctatgga aaaatcaatt tgtttgatgc ggctattgct caatttgata tctatctcct
    43141 cgccgggggt ggccaaatag aacttgaaag cggtaactct cctcttgcta cgggcggagt
    43201 cggtttcgga ttgtggatga caaaacatct ttcagcccgc gcggagtttc gctatcaggc
    43261 ttaccaagat gaaatcgtaa ctgggcaacg ggacattaac gccatggtgg ccacagtcgg
    43321 actcggatgg attttatgat caccttatcg atctttaagc aattggctct agccttggtg
    43381 atcggcatgg ctcatgccga agatcgtctg tcagcgcaaa aatggtctga agattttatc
    43441 aagactccat cacttgattt cgaaaaactc gacaagaaaa ttcttggcgc ttgtgaagac
    43501 tgtgaaaata aaagcaaaat gcaaacagca cgtaaactct tcgccaaagg ccaatacgac
    43561 gaggctcgcg cactctacgc tcaagtcagt aaaggcagtg cttactggtt agaagcggtt
    43621 gaagaaaaag cttggactta cttccgtcaa aatgagctgg aatcagccat ggcccaagcc
    43681 aaaactcttt tggctccgca atttgaaaat tacgtcggct ctgaatccta ctttatgcaa
    43741 tcactggcgc agctgaagtc ttgtaactat aaaggggttt ttgaaaccaa tcgtctcttt
    43801 aaagaaaaac aaaaaactca cttggcagaa attcaagctc tctccaaaac cgggatgaat
    43861 gaggcgctta aaaacgtgat gaacgaaacc gataaattcc ccatgagatt taccgatgtt
    43921 ggagaaaatg cttctcactt gccgcatctc ttcttcaaag atatcgaatt tcaacgtcaa
    43981 ttgatgagat ataaattatc cgagcaagct ctggctgctt taaaagcaga ggatcgcgca
    44041 tccgccctgc aaagcactct tgaaaaagtg aaatcggcaa gcgcctcaca attacagaat
    44101 cgcatcaaag agttggcggt tcaagaaacc aatgacaatt ttaaaatcgt gcaaaaatta
    44161 aaccttgtcg aaattgaagc tattcaaaga cttcatgccg acttggatct tgataagagt
    44221 ctcttcaaaa aaggtccttt cgctaatacc aattcagacc aattggtctt tgtggacgac
    44281 ggtcggccgt ggattgatga actcgataaa tatgaagttc gcaccaaaac ttgcccacaa
    44341 aatcttagga ggaaaatgtg aaaactccga tgaaatattc gacacttctt atgacagccc
    44401 tgagcctgag ctttcttgcg gcatgcagcc ctgaaagcgg ttccttagta gcctctaagc
    44461 cttacgctca gccgactccc cccacttatg cccctgtgcc agaacaaaaa tcaggttgga
    44521 ttacagaaga tgggggacaa agccaacttg atttcaatcc gcaagtggat atcctgttcg
    44581 tatcggataa ctcagacagc atgaaatccg cacaagcgaa cttggtgcgc aatatagata
    44641 aattcgctga tggaattata aaaaataaaa tggtggatta ccatatcggt gtcgtttcca
    44701 gctgggacag ctcggagcgt ttcttgacta ccaagaaaga ccagtacgga cttggcgagc
    44761 ttcacttcgt gaaagactca aaaaatcaaa cgatcaacaa gcgttttgtg agccgtaccg
    44821 aaaaagactt aatggcttct actttggaca tcggcgtcgc atcttatgcg gatggtgggc
    44881 cagagaacga ggaattcttt tcgcctctgg ttgcggccat ggaaaaatca ggtaacggtg
    44941 gagtcaacga cggattcttc cgcgcaaacg cccagctagt ggtgatcttt ctaaccgacg
    45001 ccgatgattc aagcactgca ttgacgacgg atgaattaga tcgaaaattg cttaatttca
    45061 aaggcggaaa acgcgacaaa ctgtcggttt acggagtgtt ggtgaatgcc aacgacgacg
    45121 atcaatataa agactggggc ctgcgtattc acccaaaata tcatccggag tgtttcaatg
    45181 taacaaagaa atctcccact agaaatgatg tctgtaaaaa agggtttggt ccggatcgca
    45241 ttgagcaatt gattgttact gccaaccaag gctctggctt acccgcgaaa gaaattcgca
    45301 agaaatatat tatgagcatc gttagtaaaa actttggaac tgatcttgca accataggtt
    45361 cagacatcaa aataaggacg atggccaaag aaatcttctt aagccaagtt cccgtcgtca
    45421 ccgaaaatca acaagtacaa gttcgcgttc gctacggaac tcccaaagaa cttgctgcag
    45481 gtcgtggcca actcattccc caaagcgcaa aggggggttg gttgtataac ccagaaagca
    45541 attctgttcg cctttctgga gatatcaact ataactatga agatggcgcc cgcttcgccg
    45601 tcgacttgat gccggtgatt ttgaagtagt tatgagaacc accaatattc ttctcttcac
    45661 acttttcgct ttttccgctc acgctcaagg cagagcttta tccatagaac aggttttgga
    45721 tgcggctgga tcgggaaaac tggttacttt aaaaaaatat catagtaaaa aaggcgacct
    45781 caacgcacaa gattctcacg gcctcacacc attaatgcag gccgtagcca ctggacaaaa
    45841 aaaagtagtg gattatttgc tccagcagaa agtgaatcta gaattgaaaa atgcgaacgg
    45901 cgacaccgcg ctagccatgg ctattggcaa tgaacaagat caaatcgccg tagccctgat
    45961 caatgccggc gcaaaggtcg atgttctcgg cggcgaagat aaaaacaatt tggtttttat
    46021 ggcggcctcg gtcaatgcga caaagactct gcagctcctg actaaaaaag ctccagagca
    46081 aatcaatatg aaaaataaaa acggcgatat gccattgcac gaagccgcac gtttcggcac
    46141 cgagaaaact ctagaagttc tcctgaatgc gggagcaaaa aaagaaatcg ttaacaattt
    46201 tggtaaaaca ccccttgacc tcgccaagtc tattaataat aaagcggcgg tgagggtttt
    46261 gtctcgaacg acaccatagc catgacaagt ctatagtctg tactatagca atatactgat
    46321 atagtttatg ttgataacta tatcagtata ttgctatagt tattaacatg acttatagtt
    46381 taaacaagca tgagcttcag caaattttgc gtgatcaaag gaaggaaatt gaatcccttc
    46441 actcgaaatg gcttcccagg gaaaagcttc atgaattcaa atctctttta tcctctaaac
    46501 tagttaaagt tgttatgggg attcgacgtt caggaaagtc tactctctgt attcatgcgc
    46561 ttccctttga gcagtttgtt tatatcaatt ttgatgacga acgactcatt cgactgacca
    46621 ccaaagactt acagacagtt tacgaagtca tgactcaact aaatcctcaa gcccaattct
    46681 atatctttga tgaaattcaa aacattgata gctgggagct ttttataaac cgactaaagc
    46741 gtaaaggact caatattatt gtcacaggaa gcaacggaca actcttgagt aaggagcttg
    46801 ccactcactt gaccggccgt catttaagct tggaattaat gcccttttct ttttacgaat
    46861 ttcttggcta taaaaacgcc agtatagaat taaatgaagc ttcgtc
//
LOCUS       DATBTE010000034         7718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_345805, whole genome shotgun sequence.
ACCESSION   DATBTE010000034 DATBTE010000000
VERSION     DATBTE010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345805"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="VIG33_01435"
     CDS             <1..1168
                     /locus_tag="VIG33_01435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8269523.1"
                     /translation="LEYFPLRGEENRVDGVVVVASDITSLVEARKQAENEKQHAKLII
                     NIVRSKNEMGRFIREAQMLLKELHQATQVSQPNLNSEIIFRCLHTLKGNSALFSVHTM
                     AETCHHAETRLVEYNENKTPANAELLKSQCQVVELQFDIFLAEIKEILGSTIVSNERM
                     LEIPVLQINNLLEKLSEIPQAKAIAENTFGYLLYEPIKNFFDPYKEVSYKVAQSENKI
                     IRNIEFKNNMIPVVPEIYGPLFAAFVHAFRNAVDHGIETPKNRQKAGKPEGGEILVTF
                     ERQDLPTPKLKISISDDGQGIDPQKIRAKLARHGLEATTESDTQVIQHIFDSQFSTKE
                     TITQTSGRGIGMDAIKYEAHQLGGSVWVESVIGKGCTLIVEVPYQTKIPAKKAA"
     gene            1336..4140
                     /locus_tag="VIG33_01440"
     CDS             1336..4140
                     /locus_tag="VIG33_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEY8269524.1"
                     /translation="MNSKKILFSLFALILGTVGCQKMTAGTDTQLDSANNGLATRCPS
                     ATAYIDPSLLQIEGTPVAQKGDQVTYQLSEDLGCSPSQKVSWTTIGNSQAVKVGAAMT
                     SSFQKAGEYVVVAKIEGSQPTSSHSSAPTTFKSTSDTSSTISFKTVVVSTQAVISGPD
                     IAMVGSPVSYSLAVPAGLALRSADWDFGDSSSFESSLGPVSHTYGRPGQYQISVKTVD
                     SNGVQNLLTHQVNVLMIQDGLECVSDLAISGPAEAVVGIPVSLSLYIPSCLTSKITAV
                     KWNFGDNSTSNNQSVQHTYQSVGDYQIAVQIFIDSSPTPWVTLVHDISANPPTPTPTP
                     TPEPTPINPNACSTAGEKRSSQSELYSGNSACGIDGIKTMSYRDVTVEECKLTNNGLE
                     WTQVSKTKELQSEGSCQGQSCTLPDGSLLKNGGSKIYFSTSTPTGSCSSASQIRACNN
                     GVLSGSSEAIYTICHEGCGDFGSHGTTKTNIITGETNVALTCEFGEQGYFSVFNQVSD
                     QICKEGQIVTSNTHQGDIKTQGLCPIYSYVPSELWTSCSADCGGKQSRLFTCKDDKGI
                     EAPAERCKAAPIEERLCDGNPAAVTREEHAVTQEEANSSERCPANQIGVIAKSREVTT
                     TTTFACIDHSIQQAKEEISASPWVSEKYCRTYVAHRCSGDSITNNEAIARYKWMVKCQ
                     DQIPVVKEFLEQFKDLGVKIEGKKVEIDTKSRVLYPTFLNRATKPEKVWIAPKVATAS
                     CSVPSTIYIASVCVSSCATPEQQILVDQQLKGSPKYVSFIEALTKNYHYVATLKNASS
                     MHSKSIQKTAVDQWVTELLDGDHDIIEFKMKSGRSLRLTPNHPLLAADGLMKAAGDFK
                     VGDKLVVLGGELDPILSLEHMNHYGKVYNLFVKSSDIKKNIVITNGYLNGTAFFQNEG
                     AENLNRKIFKHNMIQGVFSK"
     gene            4137..4832
                     /locus_tag="VIG33_01445"
     CDS             4137..4832
                     /locus_tag="VIG33_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269525.1"
                     /translation="MKTIKIALGFGTFAILVGALIHRAPSSHLTTSRNMNESPRPLFS
                     KSQRKEEVLPALPIANPNAPEQKVGTSMVLKSFEGKKDLEGYRLLSKKIFLTNQEKIQ
                     MQDLLKNDEFTRQLKKLFTVSSEYDNLENQQNIAVDFLLAAATGENGALASEILKSII
                     QDPATENESLSRTTRENLAGVKAEVLYRWSAQEPLKAAEMRQWLPGPVSEKIWKNVLA
                     QQEMNRAESAMVK"
     gene            complement(4840..5247)
                     /locus_tag="VIG33_01450"
     CDS             complement(4840..5247)
                     /locus_tag="VIG33_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8269526.1"
                     /translation="MFPLDTRILVIDDMASIRDLVKNHLKAMGFKNIQEASDGEEALR
                     LLIQFNTPGNSFQLVISDWNMPHMKGIDLLKHLRAQAEWANLPFVLLTSEAERDQVTE
                     AVLSGASQYIVKPFSAKVFEDKLRTAFQKHNKA"
     gene            complement(5332..6180)
                     /locus_tag="VIG33_01455"
     CDS             complement(5332..6180)
                     /locus_tag="VIG33_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEY8269527.1"
                     /translation="MSFLSVGAQEIPILDGVHPQNRLSLSSIVQGIQPGTIIVMGENH
                     GFKSSQQGQMELLRALRIRGLKLSVGLEFFYYPDQVLVNDFRSGLLSEVEFLQKIQWG
                     KPSFDFYRDQAQFPRYDQGEYTLALNAPRTLTGKISKVGLGGLTAEEQKMLPPDFQLG
                     RDSYKERFLAMMPHLPDAQAGERYFAAQSVWDDTMAWKAAEFMQRHPDQVLLIVVGDF
                     HGSYGGGLPDRIEARTHQRPLIFSYLNTYGLMLDEVRQELGPSAVHGPRGDYIWFTQE
                     PSGFNP"
     gene            6427..6606
                     /gene="rpmG"
                     /locus_tag="VIG33_01460"
     CDS             6427..6606
                     /gene="rpmG"
                     /locus_tag="VIG33_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEY8269528.1"
                     /translation="MAKKSGRIIITLECTEARAEGKPVSRYTTTKNKQKTPGRMEKKK
                     YNPNLQRHTVHRETK"
     gene            6610..7146
                     /gene="dcd"
                     /locus_tag="VIG33_01465"
     CDS             6610..7146
                     /gene="dcd"
                     /locus_tag="VIG33_01465"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY8269529.1"
                     /translation="MILTDQEILENMKKGFIKVEPYLKECLGTNSYDVHLGKVLGVYV
                     DSVLDAKKHNKVELFEIPEEGIILTPENFYLGVTQEYTETLKHVPFLEGKSSVGRLGI
                     DIHATAGKGDVGFCNFWTLEISVKKPVRVYAGMPIGQLIYFEIKGEVLTSYKNKSSAK
                     YNDKQAIPVESMMWKNRF"
BASE COUNT         2301 a         1789 c         1617 g         2011 t
ORIGIN      
        1 cttggaatac ttcccacttc gtggcgaaga aaatagagtg gatggtgtgg ttgtggttgc
       61 ctctgatatc acttcattag ttgaggctcg caaacaagct gagaacgaaa aacagcacgc
      121 gaaactcata ataaatatcg ttcgcagtaa aaacgaaatg ggccgattta ttcgtgaggc
      181 acaaatgctt ttaaaagagt tgcatcaagc gacccaggtt tctcaaccga atttgaattc
      241 tgaaatcatt ttccgctgcc tccacaccct caaaggaaat tcggccctct tttcagtgca
      301 tacaatggca gagacctgcc atcatgcaga gacccgtttg gtcgaatata atgagaataa
      361 aactcccgcc aacgcggaat tattgaagag ccagtgtcag gttgtggaat tgcaatttga
      421 tatatttctc gctgagatca aagaaatatt aggttctaca atcgtgtcga atgaacgcat
      481 gttagaaatt cccgtcttac agatcaataa tttgcttgag aagttaagcg agatacctca
      541 ggctaaagcc attgcagaaa atacttttgg atatttactt tatgagccta tcaaaaactt
      601 ctttgaccct tataaagagg tttcttacaa agtagcccaa agtgaaaaca aaattatccg
      661 caacatagaa tttaagaata atatgatccc ggtagttcct gaaatttatg gtcccctgtt
      721 tgccgccttt gttcacgcct tccgcaatgc cgtggaccac ggaatcgaaa ccccgaaaaa
      781 tcgtcaaaaa gcaggtaaac ctgaaggcgg agaaatcctg gtcacatttg aacgccaaga
      841 tctgcccact ccaaaactga aaatttctat aagtgatgat ggccaaggca tagatcctca
      901 aaaaattcgc gctaagctag cccgtcatgg cttagaggct acaacagaat ccgacactca
      961 agtgattcaa catatttttg attcgcaatt ctccaccaaa gagaccatca cccaaacctc
     1021 aggccgtggt ataggtatgg acgccatcaa atacgaggca catcaattag gcggaagtgt
     1081 gtgggtagaa tccgttatcg gcaaaggttg cactcttatt gttgaagtcc cttatcagac
     1141 aaagatcccc gctaaaaaag cggcttaaaa tgaagccgtg attgcaaaat ctttttacat
     1201 aaaaaataac atttctaaag ttgtgttttt tcgtcatttt cctcgacatc caactcaatc
     1261 atgttgattc actctttaag gaaaatttgt gaagttcgag atatcttgag aggatgagtt
     1321 ccaaggagcc atacaatgaa ctcaaaaaag atattattct cattattcgc acttattcta
     1381 gggactgtgg gttgccaaaa aatgacagct ggaacagaca ctcaattaga ttccgcaaat
     1441 aatggtcttg ccacgcggtg cccgtcagcg acagcctata tcgatccttc tctattacaa
     1501 attgagggaa caccggtagc tcagaaaggc gaccaggtaa cttatcaatt aagtgaagat
     1561 ctaggttgct ctccttcaca aaaagtgagt tggacaacca ttggaaatag ccaagctgta
     1621 aaagtgggag ctgccatgac ctccagtttt caaaaggctg gagaatatgt ggtggtcgct
     1681 aaaattgaag gcagccaacc aaccagctct cattcaagcg ctcctacaac tttcaaatct
     1741 acctctgata cctcaagcac aatttcattc aaaacagtgg tggtttcaac tcaagctgtg
     1801 atctcaggcc cggacattgc catggtgggt tctcccgttt cctatagcct ggcggtccca
     1861 gcaggtctcg ctcttagatc tgcagattgg gacttcggcg actcttcctc atttgaaagt
     1921 agtctgggcc ctgtgtctca tacctatggc cgtcccggtc agtaccaaat ctccgttaag
     1981 acagtggatt cgaatggagt gcaaaacctc ctcacccatc aggtgaatgt cttaatgatt
     2041 caggatggac ttgagtgtgt atcagattta gccatctccg gtcccgccga ggccgtggtt
     2101 ggcattcctg tttctttatc tttatatatt ccttcttgct taacttcaaa aatcacagct
     2161 gttaaatgga attttggaga caactcaact tcaaataacc agagtgttca acatacatac
     2221 cagagcgttg gcgactatca aatcgctgtt caaattttca tagattcttc cccaactccg
     2281 tgggtgactt tagttcatga cataagtgca aaccctccaa ctccgactcc gactccaact
     2341 ccggaaccaa ctccaataaa tccaaatgct tgctccactg ctggcgaaaa acgttcgagc
     2401 caaagcgaac tctattctgg aaacagcgct tgcggtatag atggaataaa aaccatgtcc
     2461 taccgtgatg tgaccgttga agaatgcaaa cttacaaata acggtctgga atggactcaa
     2521 gtctccaaaa ctaaagaact ccaaagtgaa ggctcttgcc aaggccagtc ttgcacattg
     2581 cccgatggct ctctcctgaa aaatggtgga agtaaaatct atttttcaac ctccactcca
     2641 acagggtctt gctccagcgc aagccagatc cgtgcttgta acaatggtgt tttgagcgga
     2701 tcttccgaag ctatttacac tatttgccat gagggctgcg gagactttgg ctcccatgga
     2761 acgaccaaaa ccaacatcat tactggcgag acgaatgtgg cgttaacttg tgagtttggt
     2821 gaacaaggtt atttcagcgt ctttaatcaa gtatccgatc agatttgtaa agagggccaa
     2881 atcgtaactt ccaacactca tcaaggggat atcaaaactc aaggcctatg ccctatttac
     2941 tcttatgttc cctctgagct ttggacctct tgttccgccg attgcggtgg caagcaatcg
     3001 cgcctcttta cttgtaaaga cgataagggc attgaagctc cagccgagcg ctgcaaagcg
     3061 gctcccatcg aagagcgcct atgtgatgga aacccagctg ccgttacgag agaagaacac
     3121 gctgtcacac aggaagaggc taatagctct gaaaggtgcc ctgcaaatca aattggcgtc
     3181 attgcgaaat cacgagaagt cactacaacg acaacttttg cttgtatcga tcacagtatc
     3241 caacaagcga aagaagagat cagtgcaagt ccgtgggtca gcgaaaaata ttgccgcact
     3301 tacgtcgctc accgttgctc aggggatagc ataaccaata acgaagcaat cgctcgctat
     3361 aaatggatgg tgaaatgtca ggatcaaatt cccgtagtga aggaattctt ggaacaattc
     3421 aaagatttag gagtaaaaat agaaggcaaa aaagtggaaa tagatacaaa aagcagagtt
     3481 ctttatccaa ctttcttaaa ccgggcgaca aaacctgaaa aagtgtggat cgcaccaaaa
     3541 gtggcgacag ccagctgctc agttccttcc actatctaca tcgcttcggt ctgtgtatct
     3601 tcttgcgcaa cacccgagca gcaaatttta gttgatcagc aactcaaagg aagccctaaa
     3661 tatgtttctt tcattgaagc gctgactaaa aattatcact atgttgcgac tttaaaaaac
     3721 gcttcctcga tgcacagcaa atccattcaa aaaacagcgg tggaccaatg ggtgaccgag
     3781 ctccttgatg gcgaccatga tattatcgag tttaaaatga aatcgggtcg cagccttaga
     3841 ttaactccta accatcccct tttagccgcc gacggtctta tgaaggccgc aggtgacttc
     3901 aaagtaggtg ataaattagt ggtcttaggt ggggaactag atccaatcct atctttagaa
     3961 catatgaatc attatggtaa ggtttataat ctcttcgtga aatcgtcgga tattaagaaa
     4021 aatatcgtga tcacaaatgg atatttaaat ggaaccgcct tcttccaaaa tgaaggagca
     4081 gagaacctga accgaaaaat cttcaaacac aatatgattc aaggggtctt ctctaaatga
     4141 aaacaataaa aatagccctc ggctttggca cctttgctat tttagttgga gctctgatcc
     4201 acagagctcc ttcttctcac ttaaccacct caaggaatat gaatgaatca cctcgcccac
     4261 ttttttccaa gtcgcagagg aaggaggagg tccttcctgc tttgccaata gcgaacccca
     4321 atgccccaga gcaaaaagtt ggcacctcga tggtgttaaa gtcttttgaa ggaaaaaaag
     4381 atctcgaagg ttatcgcctt ctcagtaaaa agatctttct gacgaaccag gagaaaatcc
     4441 aaatgcaaga tctccttaaa aatgacgaat tcacccgaca gctcaaaaaa ctttttacag
     4501 tttccagcga atacgacaac ttggaaaatc aacaaaatat cgccgtggat tttcttttgg
     4561 cagctgccac tggtgaaaac ggcgccctcg ccagtgaaat actaaagtct atcattcagg
     4621 atccagcgac tgaaaacgaa agcctgagta ggaccactcg ggaaaacctt gcgggggtaa
     4681 aagcggaagt gctctacaga tggtcagcgc aagagccctt gaaggccgct gaaatgcggc
     4741 aatggctccc cggccctgtc agcgaaaaaa tctggaaaaa cgttttagcc caacaagaaa
     4801 tgaatcgcgc ggaatccgcc atggtgaaat aacttgagct taagctttgt tatgtttttg
     4861 gaatgcggtc cttaatttgt cttcgaaaac ttttgcagaa aagggcttca caatatactg
     4921 agacgcgccc gaaagaaccg cctctgtcac ctgatcgcgc tcggcttccg aagtcaaaag
     4981 cacgaagggc aaattcgccc attcagcttg ggcgcgcagg tgtttcaaaa gatcaatgcc
     5041 cttcatatga ggcatgttcc aatctgagat caccaactga aaactatttc ctggagtgtt
     5101 aaattgaatc agcagacgaa gagcttcttc accatcactg gcttcttgaa tatttttaaa
     5161 gcccatagcc ttcagatggt tctttactaa atctctgatc gaagccatgt catcaataac
     5221 caaaattctt gtgtccagag gaaacatagc gatctcctaa ctctctatat cttttagtgt
     5281 agagagagtc aggggaagtg tacaagtcac tctttgctgt ctcgacttaa gttaggggtt
     5341 aaagccgctc ggctcttgag tgaaccaaat ataatctcct ctaggcccat gaacggccga
     5401 ggggcctagc tcctgccgca cttcatccag cataagtcca tatgtattta gataagaaaa
     5461 tatgagaggc ctttggtggg tcctggcttc aattcgatcc ggcagccccc ctccatagct
     5521 gccatgaaaa tcccctacta caatgagcaa aacctgatcc ggatgccttt gcatgaattc
     5581 agccgccttc catgccatgg tatcatccca gacactttga gcggcaaaat agcgctcacc
     5641 ggcttgcgca tccggcaagt gaggcatcat tgccaggaag cgctctttat aagaatctcg
     5701 gcccaattga aaatctggag gcagcatttt ctgctcctcc gctgtcagtc cacccaaccc
     5761 gactttggag attttaccgg tcaaagtgcg aggggcattc aaagccaagg tatattctcc
     5821 ctgatcataa cgggggaatt gagcctgatc gcgataaaag tcaaaggagg gctttcccca
     5881 ttgaatcttt tgaagaaatt cgacttccga taagagtcca ctgcgaaagt cattcaccag
     5941 gacttgatct ggataataga agaattccag gcctactgaa agctttaagc cccgaattcg
     6001 aagagccctc aacaactcca tttgcccttg ttgagaggac ttgaagccat gattttcccc
     6061 catcacaatg atagtccctg gctgtattcc ttggacgatg gaagataaag aaaggcgatt
     6121 ttgaggatgg acaccgtcaa gaattggaat ttcttgggca ccgacggaga gaaagctcat
     6181 aaggaagact ccaaaaacta agatggcatt caaggtttag ctccttagct cattttcatg
     6241 gattggtttt cagggactgg caaaattagc tgtaaatagt cattttggct gttgaaactt
     6301 acaaggtctt ttgttacctt gggccccttt aaaagggtat tgtgatctgt gtatttgacg
     6361 ctatgcccca cttgtgacta atgataacta acattattgt gacccatact gaacacggag
     6421 aatattatgg caaaaaagtc tggaagaatc atcattactc ttgaatgcac agaagcccgc
     6481 gctgaaggta aacctgtttc tcgctacact acgactaaaa ataaacaaaa aactcctggt
     6541 cgtatggaga aaaagaaata caacccaaat ttgcaacgtc atacagtcca tagagagact
     6601 aaataatcca tgatcctgac cgatcaggaa attctagaga atatgaaaaa ggggttcatt
     6661 aaagtagagc cctacctcaa agaatgcctc ggcacgaact cttacgatgt tcacctgggg
     6721 aaagttttag gcgtctatgt tgacagcgtt ttagatgcta aaaaacataa taaggtagaa
     6781 ctctttgaga ttcctgagga gggcattatc ctgactcctg aaaattttta tctcggtgtg
     6841 acccaggaat acaccgaaac ccttaagcac gtccctttcc ttgagggaaa atccagcgtg
     6901 ggtcgcttgg gaattgatat tcacgccacc gccggcaagg gcgatgttgg attctgcaat
     6961 ttctggactt tagaaatctc agtgaagaag cctgttcgtg tctacgcggg catgccaatt
     7021 ggccaactga tttatttcga aatcaaaggc gaagtcctga cttcctacaa aaacaaatcc
     7081 tccgctaaat acaacgacaa acaggccatt cctgtggagt ccatgatgtg gaaaaatcgc
     7141 ttttaattta gagaccgcat cctgacaatc ggggctctcg ccccgaaccc agaaagatcg
     7201 accatctacc gctcttactg gatgaatttt atacttattg tagcccattt tttttcgagc
     7261 attccttttg gtgaacttcc aatcaaactt aattttctct ttattcgtat ttttgttcca
     7321 agcctttact tttctacgcc gagctgcaag gtctggaact agagttcgtc atacatactg
     7381 gccaataaaa atgccaatcg tagtttcgcc tgatagagcc tactgcactc cgttttcgtg
     7441 attgctttgt gattcaaacc tgcacagttc ctcaaacggc agtcccgaga gagtactctc
     7501 gagactcttt gataaaattc gcaaaactaa ggctgacctg gatggtcagc ttttctgggg
     7561 ctcggggacc agtcttcaac accctgttag tccaaatacg gtcgcttagc acaaatgacc
     7621 catgctctat cataagtctt attagcttca gattcagagg tttcataaag ataaccttta
     7681 tcaaaataaa agaaataatg ctgaattgcc tcctcgta
//
LOCUS       DATBTE010000035        13642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1779660, whole genome shotgun sequence.
ACCESSION   DATBTE010000035 DATBTE010000000
VERSION     DATBTE010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 13642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13642
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779660"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /gene="fliP"
                     /locus_tag="VIG33_01470"
     CDS             <1..476
                     /gene="fliP"
                     /locus_tag="VIG33_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165671.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEY8269530.1"
                     /translation="LLVGLSLFLTFFVMGPAFKEANETGVQPYLAGKISQDVALENAL
                     SPLRKFMFRQTRDTDLGLFLKLAKIEAPKTRADVPTIVLVPAFVVSELKTAFQIGFII
                     FLPFLVIDIVASSVLMAMGMMMLPPVVISLPFKIMLFVLVDGWGLLIGSMVKSFG"
     gene            486..758
                     /gene="fliQ"
                     /locus_tag="VIG33_01475"
     CDS             486..758
                     /gene="fliQ"
                     /locus_tag="VIG33_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEY8269531.1"
                     /translation="MTEEIIIKLGQDALRTTAMLAAPMLLSTLVIGLAVSIFQALTQI
                     NESTLTFIPKMVVVALVFVLAGPWMMDVMSSYTITLFENIATIVRE"
     gene            770..1549
                     /gene="fliR"
                     /locus_tag="VIG33_01480"
     CDS             770..1549
                     /gene="fliR"
                     /locus_tag="VIG33_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEY8269532.1"
                     /translation="MFYTMSEAQVVMFGLILLRMISFVVSSVVFGSGTISAPVKILLS
                     LVLSMVVLPSIHLGPADSARLVEDVVLLSAREIFIGLSLGFLTRLFFFTIGMTGELIS
                     VSIGLGQAQIFNPMMGSNSNTVEQFYSTIATLIFFAANGHHLLISAIAQSYELVPLAQ
                     MKLNVGPYAEIATFTQDMMVMAIKMCAPILGAVMITNIAMGVLGRAVPQINVLVTSMP
                     VTLMLGFILMFLCIPLLVTEMHGILDLTHTKMMLVMKALAF"
     gene            1563..2627
                     /gene="flhB"
                     /locus_tag="VIG33_01485"
     CDS             1563..2627
                     /gene="flhB"
                     /locus_tag="VIG33_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HEY8269533.1"
                     /translation="MAEEQDEKTEQATDARREDFRRRGQVAHTKELASCLLLLASAGG
                     VFVLSRFFFQNIHDVFTYSFGPQLVTTIRSGNFTEALRFSGEKMFMLIAPVMAVAGAI
                     SLAATVLQIGLLQVEDAMSPDLNKLNPLEGFKRVFSLRALVEGLKSFLKLLFIGIVLY
                     FLLKSEVSKIPYLVSYSIEGIFQYIGGIVVRLFGGIGLMMLMLSLGDYFFQRWNLEKE
                     MMMTKQEVKEESKSREGDPMIKSRIRRLQRERASKRMMEAIPKADVVITNPTHIAVVL
                     KYDANLPAPQVIAKGADLIAEKIKQIAREHNIPIMENKPLARTIYKTLKIGQVIPREL
                     FVAVAEVLSYVYKLRKKARR"
     gene            2627..4726
                     /gene="flhA"
                     /locus_tag="VIG33_01490"
     CDS             2627..4726
                     /gene="flhA"
                     /locus_tag="VIG33_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEY8269534.1"
                     /translation="MEELFQFMKKFEKFTKNTDLFIAFGLLGILGVMIIPLPPMMLDI
                     ALSFSLSLSLLILVVSIYVKRALDFTSFPSLLLMTTLFRLSMNVATTRLILTHGHEGP
                     HAAGGVIEAFGNFVVGNNYVIGIIVFMILIVINFIVITKGSGRVAEVAARFTLDAMPG
                     KQMAIDADLNAGLITEADARKRRKEIEQEADFYGAMDGASKFVRGDAIAGIIITVINI
                     VGGLLIGVIQKGLDVGIAAKYYTMLTIGDGLLAQIPALIISTAAGIIVTRSSSTDEDM
                     GTEVSSQLLANPRSVAIAASVIGLLALVPGLPTIPFLTMSLGLGMLSWVIRRYQLEKE
                     NNEKKKNESAAAAPKKENIETMLPLDLIELEVGYGLINIVESDKSGDLLERIVSIRKQ
                     FALDLGIVVPSIHIRDNLQLAPGEYRVLIKGNRVAGGSLRPEFLLAMDPGNVIERIEG
                     LATKEPAFGLDALWVSPNRKEEAEMAGYTVVDLPTVMATHLTEIVRTHAHELLGRQEA
                     SVLVDNFKKTHPKVVEELIPDQLTLGSVVRVLQGLLKEQVSIRDMLTIFETLADEAPR
                     NKDIEVLTENVRKALARSITAKYTTDMGNIPVMTLHPVIEELIANSLLQTEQGVQLVM
                     DPNTAHRLIGEIARTVETHPEIAGQPILLTSPTSRRHLFKLTHRFIPQLVILSHNELT
                     SDAHVQSVALVELNHAS"
     gene            4716..6116
                     /gene="flhF"
                     /locus_tag="VIG33_01495"
     CDS             4716..6116
                     /gene="flhF"
                     /locus_tag="VIG33_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HEY8269535.1"
                     /translation="MQVKKFEARTMKEALEMVKAELGPEAIILSARDNNKTYGLVGDG
                     SVEITAAVSDETLQRKKFTEARMREQEKVKFNQSSARSQKELIEKMVNKHLEKNKAPR
                     AITSQRYAEIDEENLNVGVHAVAKQALASMRNQAAQMASVQPTPKAVLPAVKVSESSE
                     MQSLKSEIANLKQVLAHFQSVPQRFIQGHPGAEFGLPYDLSFVFEKLTHAGVAAEIAA
                     DMCARVQKDLPSLKLKNHSLVEGRVARQILDTTKTVALDETMKAKVHLFVGAAGSGKT
                     TTLVKMASHLVVRQNKKVAILSADTFKVGASDQMRIYAQILNVPFTVIRHASDWYHIM
                     NYMNSVDYILVDYPGLSLRNAEEGMNLKHLMPPATLYPRIHLVLSTLAKDQDVTELGR
                     RYSVLDYQDVIFTGLDESVQHGTIYNFMKRFDIPLHSFGIGSRVPEDFEFATKERVLD
                     LLFKITQAKKQEQQTL"
     gene            6113..6943
                     /locus_tag="VIG33_01500"
     CDS             6113..6943
                     /locus_tag="VIG33_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEY8269536.1"
                     /translation="MKYNGLFQSRSTRTISITSGKGGVGKTTLTANLALRLSQTGNKV
                     LIFDGDLGMANVDILFGVKTEGNIHDVLRGNKAITEVMVEVAKDVFLIPGGSGILEFN
                     HLNGFQRRAIVEAVGTLPQDFDYMLIDTAPGIAENVLYLNAAAQSICVIITPDPASLA
                     DSYALIKVLHQKYKENKFSIICNNVRDEIDGAGLYQRFNDVVNKFLCIGLDYWGTVPM
                     DPVLRRATQMNRLIMRYDPSAESAKAIKNLCAQVENSSQKIITKGGLQMFWEQVVGLA
                     "
     gene            7012..7797
                     /locus_tag="VIG33_01505"
     CDS             7012..7797
                     /locus_tag="VIG33_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HEY8269537.1"
                     /translation="MAKNVALLKKYKDEPKKITQKHKDELIKEYAPLIKFVAQKIAVR
                     LPSNIELDDLISAGVIGLMDAIDKYDPSHDNKFKTYAEFRIRGAILDELRAQDWVPRS
                     IRDKAKLLDRTMASLENDLGRAPTEEELSKALNITVEEFHDLVNQVRPVSLLSIDNSQ
                     SFSNDDHKSILNLLEGSKLNNPFNQLNIKSIKLVVTQAIEDLPERQRLVLSLYYYEDL
                     NLKEIGQILRVTESRVSQLHAQAVARLRVKLQQSIGAGELEVA"
     gene            complement(7819..8892)
                     /locus_tag="VIG33_01510"
     CDS             complement(7819..8892)
                     /locus_tag="VIG33_01510"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HEY8269538.1"
                     /translation="MTKLKPKEGLAKKTTAKPPKSVSSKKPAKAKSVFAEIVDEDSIA
                     PKIQADKVYAVPTDEDIEDSLKMKELSPKSLVAANHNKAVTTTDPLAIYLQDVRKYPV
                     LSKEEEQEIAKKYFETKDPESAQILIKSNLRFVVKIAAEYSKFGAKMIDLVQEGNIGL
                     MHAVREFNPYKGARLITYAVWWIRGYIQEYLMRQYSMVRIGTTQNQRKLFYQLQKEKD
                     ALDSLGQEPNIALLSSRLGIPEDEVEQMTQRMTGRDISLDRPLDEDAGGTLMDLQRNT
                     SDMSLDEKIAHEEELEILKRKLKEIRNELSEREKIILDERLLNDEPLTLQEIGEKHGI
                     TREAVRQMEVRVLKKIRTKMEEK"
     gene            complement(8923..9228)
                     /locus_tag="VIG33_01515"
     CDS             complement(8923..9228)
                     /locus_tag="VIG33_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEY8269539.1"
                     /translation="MPKFTIEHKSSQSAKEAFDKIKTFMTEDEDLRRFDSKMQCQFND
                     DSMNGLVKGGQFKADISVLGQGTGSKIQIVVDLPMLLTPFKGKVQETLQKKLSKYLA"
     gene            9273..11108
                     /gene="aspS"
                     /locus_tag="VIG33_01520"
     CDS             9273..11108
                     /gene="aspS"
                     /locus_tag="VIG33_01520"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY8269540.1"
                     /translation="MSDSKTMKFVKDLKRTEYCGSINSKFIGQKVVLMGWVDSRRDHG
                     SLVFIDLRDREGIVQVVLDPSKSETATSKNLRGEYVIAVEGVVRARPEGMKNTKVKTG
                     EIEIEATRCDILNEAAVPPFQVDDKNVNETLRLKYRYLDLRSPRLTNHLMIRHKVAQL
                     VRRFLSDNGFIEVETPILFKSTPEGARDYLVPSRVNQGNFYALPQSPQILKQLLMVSG
                     YDRYFQIARCFRDEDLRADRQPEFSQIDMEMSFIDQEDIMQLNEKLLCTIWKEIKGIE
                     VGVLPRMTYQEAMDRFGNDKPDTRFGMEIKDLKGIVTGCGFKVFDDVIARNGIVRGIP
                     VPKGGSFSRGQFDKLTDIAKRAGAKGLVWIKAEADGIYSSPVSKFFTSEKLGEIFKAV
                     GAQSGDCALIIADDFDPACAALSTMRLHLGKELNLIDTSKYNFLWVVDFPAFEYSPDD
                     KRWVSRHHPFTSPKDEFSQDLIDGKESSYGNMLAKAYDLVCNGYEMGGGSIRIYRTEL
                     QQAMFRLLGMGKEEQESKFGFLLDALKYGAPPHGGIAWGLDRLVMLLCNTDAIREVIA
                     FPKTAKASCLMSDCPSEVSRDQLTEVGIKLTSLAEKAVEEAKKIL"
     gene            11139..12212
                     /locus_tag="VIG33_01525"
     CDS             11139..12212
                     /locus_tag="VIG33_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEY8269541.1"
                     /translation="MSQPGTKLFTPIRLRELNIKNRIFMSAMCQYSAIDGVPNNWHLV
                     HLGSRAVGGAGLIMVEATAVRPVGRISPADLGIWNDHQMEAYRPIADFLKVHGAIPGI
                     QLAHAGRKASTAASWKGGRPIEGKHGGWVPEGPSPIPFSAEHAVPRELTKSDIEALVE
                     DFASAAKRSLEAGFQVVEIHAAHGYLMHEFLSPLSNHRTDEYGGSIENRMRFPLEVAE
                     RIRKIWPAKWPVFVRISATDWVEGGWDLPQSTVFAKQLKDIGIDLIDCSSGGNSPQAR
                     FSPGPGYQVAFATAIREQAQIFTGAVGVITSGLQAEKILQEGQADVIFVGREFLRDPY
                     FPLHAARELGVDLPWPPQYDRAK"
     gene            complement(12231..>13642)
                     /locus_tag="VIG33_01530"
     CDS             complement(12231..>13642)
                     /locus_tag="VIG33_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269542.1"
                     /translation="IILAAWLVATRFIKSSLLQIAYILFVWWCGFYISRSHQHLTLLS
                     MIWGMQFVLYAVLTLDPRNLKNVLTAGLLLGLSYTGSFQNIPGLFLISLALVLYTLWK
                     LRRDLANSRSLLNIFMGLVLASIIFFGLWGPMISFTLENGVVPLASQRQLYNLDLLSP
                     LIPSENNLLYSWWPALIKLSLERNNSFDPLILILVLASLFTRKFWKEPLRILILIIGI
                     LYFILSLGPELRIANEVFYYLDFNSEIFNHFPLNVTRTPGRLAVITNLCFIFLAFLFL
                     DQWKASGDVSRNRFKKCFSVALIAWPLALGPLMNDMWFFPTLNYTSILPITGLTTLRN
                     LPPDTLVVQIPSAWAQDPSQNFNQILHDKKITSGYLAYPLYNKEVVENLMKDDFLGKM
                     GCDGEATAYTNAPLFTNPDSLHQHLMVHKYRGFIINKQILTQNPACKNLTAWVTLFLK
                     LPWVKVSEENKLFVVGEVQ"
BASE COUNT         3652 a         2809 c         3293 g         3888 t
ORIGIN      
        1 agttgttggt ggggttatca ttatttttaa ctttcttcgt tatgggtccg gcttttaaag
       61 aagccaacga aacgggagtg cagccttatc tcgcagggaa aatatcccaa gatgtggcgt
      121 tggaaaacgc tttatctcct ttgcgtaaat tcatgttccg ccagacacgc gatactgatt
      181 tgggtttatt tttaaaactt gccaaaattg aagccccaaa aacccgcgcg gatgtaccga
      241 caattgtttt ggtaccggcg tttgtagttt ccgagctaaa aacagctttt caaattggtt
      301 ttattatctt tttgccattt ctggtgattg atatcgttgc ttccagcgtc ttaatggcca
      361 tgggtatgat gatgttacca ccggtggtga tctcgttgcc ttttaaaatc atgctttttg
      421 ttttggtcga tggctggggt cttttaattg gttctatggt taaaagtttc gggtaggagc
      481 aatttgtgac agaagaaatt attattaagt tgggccagga tgctcttcga accacggcaa
      541 tgttggccgc ccccatgttg ctcagcacct tagtgattgg attggccgtg agtatttttc
      601 aagcgctcac tcaaatcaat gagtctactt tgacatttat tccaaaaatg gtggtggtgg
      661 ctttggtatt tgtgctggct ggtccatgga tgatggatgt tatgagcagc tacacgatca
      721 ccttatttga aaacatcgca acaattgtac gggaatagag gagagttcat tgttttatac
      781 aatgtctgaa gcacaagtgg tcatgttcgg actcatttta ttgaggatga tttcctttgt
      841 ggtgtcttcg gttgtatttg gttccggtac tatcagtgca ccggtgaaga ttcttttgtc
      901 cttggtttta tcaatggtgg tgctgccaag cattcacttg ggaccggctg attccgcacg
      961 tctggtagag gacgtggtgt tgctttcagc ccgtgagata tttatcggat tgagtctggg
     1021 ttttttaact cgtctgttct ttttcaccat cggaatgaca ggggagttaa tttctgtttc
     1081 catcggattg ggacaggcgc aaattttcaa tcccatgatg ggatcgaata gcaatacggt
     1141 ggaacaattt tacagcacca tcgccaccct catcttcttc gcggcaaacg gacaccatct
     1201 tttgatcagt gctattgcgc aaagttatga gttggttccg ttagcgcaaa tgaaactaaa
     1261 tgtgggtcct tatgcggaga tcgccacttt cactcaagat atgatggtca tggccatcaa
     1321 gatgtgcgcc cccattctgg gcgcggtgat gatcaccaat atcgcaatgg gggtcttggg
     1381 ccgcgcagtt cctcaaatca acgtattggt tacgagtatg ccagtcacat tgatgttggg
     1441 ttttatttta atgtttttat gcatcccgct tctggtgact gaaatgcacg ggatattgga
     1501 tttgacacat acgaaaatga tgttggtgat gaaagcctta gcgttctagg aaggaacatt
     1561 aggtggcaga ggagcaagac gaaaaaacag aacaggcgac ggatgcccgg agagaggact
     1621 tccgacgccg tggtcaagtg gcgcacacga aagagttggc gtcgtgcttg ctattgctgg
     1681 catcggctgg gggcgtcttt gtgttaagcc gatttttctt tcaaaatatt cacgatgttt
     1741 tcacctattc ctttggtcct caactggtca ctaccattcg ttctggaaat tttacggagg
     1801 ccttgagatt tagcggagag aagatgttca tgttgatagc tcccgtgatg gctgttgccg
     1861 gagcaataag cctggcggcc accgtattgc agataggcct tctgcaggtg gaagatgcca
     1921 tgagccccga ccttaacaaa ctgaaccctc tggagggatt caagcgtgtc ttcagcttga
     1981 gagctttagt cgagggtttg aaatcctttt taaaactcct ttttatcggc atcgttttat
     2041 atttcctttt aaaatctgag gtttcaaaga tcccttatct tgtgagctat tcaatagaag
     2101 gaatttttca atatatcggt ggcatcgtgg tccgtttatt cggcggcatt ggattaatga
     2161 tgttgatgct ttccctaggg gattattttt tccaacgttg gaatcttgaa aaagaaatga
     2221 tgatgaccaa gcaagaagtc aaagaagagt cgaaatcccg agagggggat ccgatgatca
     2281 aatcacgcat ccgccgtttg caaagggagc gcgcaagcaa aaggatgatg gaggccattc
     2341 ctaaagccga tgtagttatc actaatccga ctcatattgc tgtggttttg aaatatgatg
     2401 ccaatctccc ggctccgcaa gtgattgcca aaggcgcgga cttgatcgct gaaaagatca
     2461 aacaaattgc tcgtgaacat aatatcccta ttatggagaa taagcctctg gccagaacca
     2521 tctacaaaac tctgaaaatc ggtcaagtga ttccacgcga actttttgtc gccgtggcag
     2581 aagttctttc ctatgtttat aagttacgta agaaggcgag aagataatgg aagagctgtt
     2641 tcagttcatg aaaaaattcg agaaatttac taagaacacg gatctgttca ttgcctttgg
     2701 acttctcggg attttgggag tcatgattat tccgctcccg ccaatgatgc tggatattgc
     2761 cttgagtttt tctttgtctt tgagcctttt aattttagtt gtttctattt atgtgaaacg
     2821 tgcccttgat tttacttctt ttccgtcact tcttttaatg acgaccctgt ttcgactgtc
     2881 gatgaacgta gccaccaccc gtttgatttt aacccatgga cacgaaggcc cccatgccgc
     2941 aggcggtgtt atcgaggcct tcggtaactt cgttgtcggt aataactacg tcattggtat
     3001 tatcgtattt atgattttga tcgtgattaa ctttatagtt atcaccaaag gttccgggcg
     3061 tgtcgctgag gttgccgcac gtttcacttt ggatgcaatg ccaggaaagc aaatggccat
     3121 cgatgccgac ttaaatgccg gcttaattac cgaagccgac gctcgcaagc gtcgaaaaga
     3181 gattgagcag gaagccgact tttatggagc catggatggt gccagcaagt ttgttcgcgg
     3241 agatgccatc gccggcatca ttattacagt tattaatatt gttggtggtc ttttaattgg
     3301 tgtgattcaa aaaggcctcg atgtcggcat cgctgccaaa tactatacca tgttaaccat
     3361 cggtgacggt ctcttagcgc agatccccgc actcattatt tctaccgcag ccggtattat
     3421 cgtgactcga agttcaagca ccgatgagga catgggaacc gaagtctcaa gccaactttt
     3481 ggcaaatcct cgctctgtgg cgattgcagc ctcggtcatt ggtctcttgg ctttagttcc
     3541 gggtcttccc accattccgt ttttaaccat gtctttggga ttagggatgc tgtcctgggt
     3601 gattcgacgt tatcaattgg aaaaagaaaa taatgaaaag aaaaaaaatg aatcagcggc
     3661 agcggctccg aagaaggaaa atatagaaac gatgttgcct ttggatttaa ttgaactcga
     3721 agtcggttat ggcctgatca atattgtgga gtctgataag agtggagatt tgctggaacg
     3781 tatagtcagt attcgcaaac agttcgcttt ggatctaggg atcgtagtac caagtattca
     3841 cattcgtgat aatttacaac tagccccagg agaataccga gtccttatta aagggaatcg
     3901 cgtcgctggg ggctcactcc gtcctgagtt cctgttggcg atggatccag gtaatgtcat
     3961 agaacgcatt gagggccttg ctaccaaaga gcccgcattt ggacttgatg ctctatgggt
     4021 ttctccaaat cgcaaagagg aagccgagat ggccggttat acggtggtgg atttgcctac
     4081 tgttatggcg acgcatttga ctgaaatcgt tcgtacccat gcccatgaac ttctggggcg
     4141 tcaagaagcc tcggtcttgg tcgataactt taagaaaacc catccaaaag tggtggaaga
     4201 attgatcccc gatcaattga cactgggatc cgtcgtccga gttcttcagg gcctgttaaa
     4261 agagcaggtt tccattcggg atatgctgac tatttttgag actctggcgg atgaagctcc
     4321 tcgaaataag gatatagaag tgctcactga gaatgtacgt aaagccctgg ctcgctctat
     4381 tacagcgaaa tacactacgg atatgggaaa tatcccagtc atgactttac atccagtcat
     4441 tgaggaattg attgcgaact cgctgctgca aactgaacag ggagtacaat tggtgatgga
     4501 tccaaatacg gcccatcgtt tgataggaga gatcgctcgt acggttgaaa cccatccgga
     4561 gatcgcaggg cagccgatct tattgaccag cccgacctcg cggcgacact tgtttaagtt
     4621 gactcacaga ttcatacccc agttggtcat tttatctcat aacgaattaa cctccgatgc
     4681 tcatgtacag tcggtggctt tggtggagtt aaaccatgca agttaagaaa tttgaagctc
     4741 gtaccatgaa agaagctctg gaaatggtca aagcggagct ggggcctgaa gcgattattc
     4801 tttcagccag ggataataat aaaacctatg gtctagttgg tgatggcagt gtcgagatca
     4861 ccgctgcggt ctcggatgag actctgcaaa gaaaaaagtt cacggaagct cgtatgcgtg
     4921 agcaagagaa agtgaagttc aatcaaagtt ccgcgcgttc acaaaaagag ttgattgaaa
     4981 aaatggtcaa taaacatttg gaaaaaaaca aagctccccg tgcaatcact tcccaacgtt
     5041 acgctgagat tgatgaagaa aatctcaatg tgggggtaca cgcagtggcg aagcaggcat
     5101 tggcttcaat gaggaatcaa gccgcgcaga tggcgagtgt tcagccaaca cccaaagcgg
     5161 tgttgccagc ggtgaaggtg tcggaatcgt cagagatgca gtctttaaaa agtgaaattg
     5221 cgaatctaaa acaagttcta gcccattttc aaagcgttcc tcagagattt attcaagggc
     5281 atccaggcgc ggaattcgga cttccttatg atttgagttt cgtttttgaa aagttgaccc
     5341 acgccggcgt cgccgcagaa atcgccgctg atatgtgtgc ccgtgtgcaa aaggatttac
     5401 cttctctgaa attgaagaat cattccctgg tggaaggtcg ggtggccaga cagatcttgg
     5461 ataccacaaa aaccgtggct ctcgatgaaa cgatgaaagc gaaggttcac ttgtttgtcg
     5521 gagcggcagg ctctggaaag accacaaccc ttgtaaaaat ggcgagtcat ctggtggtgc
     5581 gccaaaataa aaaagtggcc attctctcgg cggatacttt taaagtcgga gcttctgatc
     5641 aaatgagaat ctatgcccag attttaaatg taccttttac tgtaatccgt catgccagtg
     5701 actggtatca tattatgaac tatatgaata gtgtcgacta tattctggtg gactatccgg
     5761 gactgagcct tcgcaatgcc gaggagggta tgaacctgaa gcatttaatg cctccagcga
     5821 cgctttatcc acggattcat ctggtgcttt cgactttggc aaaagatcaa gatgtgacgg
     5881 agctgggacg acgttactcc gttttggatt accaagatgt catattcacc ggtcttgatg
     5941 aatccgtaca gcatggaact atttataatt tcatgaaacg ttttgatata ccccttcact
     6001 cttttggcat tggttcccga gtccccgagg acttcgagtt cgccacgaaa gagcgggtcc
     6061 ttgatctatt gtttaaaatc actcaagcga aaaaacagga acagcagaca ctatgaaata
     6121 caatggattg tttcaaagcc gcagcactcg caccatcagc atcacctccg gcaaaggcgg
     6181 tgtcggtaag acgacattaa ccgccaactt agccctacgt ttatcccaaa ccggtaacaa
     6241 ggttttaatc tttgacgggg atttgggaat ggcgaatgtg gacattcttt tcggcgtaaa
     6301 gacggaaggc aacattcacg atgtcctcag ggggaataag gcgatcactg aggtgatggt
     6361 cgaagttgcc aaggatgttt ttttaattcc ggggggcagt ggcattttgg agtttaacca
     6421 tctcaatggt tttcaaagaa gagcgatcgt cgaagcagtg ggaacactcc ctcaagattt
     6481 tgattatatg cttattgata cagcgccagg aattgccgaa aatgttctat atctgaatgc
     6541 ggctgcacag agtatctgcg tgattatcac tcccgatccg gcgagtttgg ccgattcata
     6601 tgctctcatt aaagtccttc atcaaaaata caaagagaat aagttctcta ttatttgcaa
     6661 caatgttcgt gatgagatag atggcgcagg gctttatcag cgctttaatg atgtggtaaa
     6721 taaatttcta tgtataggtc tggattattg gggaaccgtt ccgatggacc cagtgcttcg
     6781 gagagccacg caaatgaatc gcctcatcat gagatacgat ccatcagcgg agtcagcaaa
     6841 agccattaaa aatctctgtg cgcaggttga gaattctagt cagaaaatca tcactaaggg
     6901 cgggttgcag atgttctggg agcaagtcgt cggtttagct taaccgtagg aaactaattt
     6961 agacttttga aagtgctcat caattgttaa gatgaaataa tcgagggaag tatggcaaaa
     7021 aacgtggcat tgctgaagaa gtacaaagat gagcccaaga agatcacgca gaagcacaaa
     7081 gatgaattaa taaaagaata tgctcctctg attaagtttg ttgcccagaa aatcgccgtc
     7141 cgtcttcctt caaatattga gcttgatgat ttaatttcag ccggtgtcat aggcttgatg
     7201 gatgccatcg ataaatatga tcccagccac gataataaat ttaaaaccta tgctgaattc
     7261 cgtattcggg gagcgattct ggatgagctt cgcgcccagg attgggtgcc tcgctccatt
     7321 cgtgataaag caaaacttct ggatcgaacc atggccagct tagaaaatga tttaggacga
     7381 gcaccaacgg aagaagagct ttctaaagct ttaaatatta cagttgagga atttcacgac
     7441 ctggttaatc aggttcgccc ggtcagtttg ctttctattg ataacagtca atctttcagc
     7501 aacgatgacc ataagtcgat tttaaatttg cttgagggct ctaagctcaa taatcctttt
     7561 aatcaattga atataaaatc catcaagctc gtggtgactc aagccattga agatttgcct
     7621 gagcgccaaa gattggttct ttctttatat tactatgaag atttaaatct gaaagagatc
     7681 ggtcagattc tgcgggtgac tgagagtcgg gtctctcagc tccatgctca agccgttgcc
     7741 cgcttacgag tgaagctgca gcaaagcatt ggcgccgggg agcttgaggt cgcttaaacg
     7801 ctttcgcccg attcaatttt atttttcttc catcttggtc cttatttttt taagtacgcg
     7861 tacttccatc tggcgaacgg cctcgcgggt gatgccatgt ttttcaccga tctcttgcaa
     7921 agtcaggggt tcatcattca gaagtctttc gtcgagaata atcttttcac gctcggaaag
     7981 ctcgttgcgg atctctttga gttttctttt gagaatttcc agttcttcct catgagcgat
     8041 cttttcatcc agactcatat cggaagtgtt tctttgcaga tccataagtg taccgccagc
     8101 atcttcatcc agagggcgat ccaagctaat atctcggccg gtcattcttt gggtcatttg
     8161 ttccacttcg tcttcgggta tgccaagacg agaactaagg agagcaatat tcggttcttg
     8221 ccctagggaa tccagagcat ctttttcttt ttgtaattga tagaaaagtt ttctttggtt
     8281 ttgagttgtg ccaatacgaa ccatggagta ctgacgcata agatattctt gaatgtagcc
     8341 acgaatccac cagacagcat aggtaatcag tcgagcgccc ttataaggat tgaattcgcg
     8401 aaccgcatgc atcaagccaa tattgccttc ttgaaccaga tcaatcattt tcgcgccgaa
     8461 tttagagtac tcagcggcaa ttttaacgac gaagcgcaga ttcgatttaa taaggatttg
     8521 cgctgattca ggatctttcg tttcaaaata tttttttgct atttcttgtt cttcttcttt
     8581 gctgaggacc ggatatttac gcacatcttg aagataaatc gccaaaggat ctgtggtagt
     8641 gacagcttta ttgtggtttg cggcaaccag gcttttaggc gacagttcct tcattttaag
     8701 ggaatcttcg atgtcctcat cggtaggaac agcgtagact ttgtctgcct gaatttttgg
     8761 agctatggaa tcttcatcga cgatttccgc aaagacgctt ttggctttag caggtttttt
     8821 gctagaaact gatttaggag gcttcgcagt tgtttttttt gcgaggcctt cttttggttt
     8881 cagctttgtc actttttttt ttgaactcaa tgccatagat agttaagcca agtatttaga
     8941 cagtttcttc tgcaaagttt cctgaacctt acccttaaat ggggttaaaa gcatagggag
     9001 atcgacaaca atttggattt tactccctgt gccttggccc aggacagaga tatccgcttt
     9061 aaattgaccg cctttaacga ggccattcat tgagtcgtca ttaaattgac actgcatctt
     9121 ggaatcaaag cgtcggaggt cctcatcttc tgtcatgaaa gttttgattt tatcgaaagc
     9181 ttcttttgca ctctgtgagc tcttgtgctc gatcgtaaat tttggcattt ttgaatttta
     9241 caatagttgg tttttgactg caataagaca tcgtgtcaga ctctaaaact atgaaattcg
     9301 tcaaagatct caaaagaaca gaatattgtg gaagcatcaa ttcgaaattt attggccaaa
     9361 aggtcgtact tatggggtgg gttgattctc gccgcgacca cggaagtttg gtcttcatcg
     9421 atctgcgcga tcgtgaaggc attgtccaag tagtgttgga ccctagtaaa agtgaaacag
     9481 cgacttccaa gaatctgcgt ggagaatatg tcattgcagt cgaaggagtt gtacgcgctc
     9541 gccctgaggg gatgaaaaat actaaagtga aaacgggtga gattgagata gaagcgactc
     9601 gttgcgatat cttaaatgag gcggcagtgc ctccgttcca ggtggacgat aaaaacgtga
     9661 atgaaacttt acgcttgaaa tatcgttatt tggatctccg ttctcctcgc ttgactaacc
     9721 acttaatgat tcgtcacaaa gtggctcaat tagtgcggcg ctttttatct gataacggtt
     9781 ttattgaggt cgaaactccg attcttttca aatcaactcc cgaaggagca cgcgattatt
     9841 tggtcccttc tcgtgtgaat caaggaaatt tctatgcttt gccacagtct ccacaaattt
     9901 taaagcagct tttgatggtt tctggttatg atcgttattt ccagatagct cgctgcttcc
     9961 gtgatgaaga cttacgtgca gatcgtcagc cggagttttc tcagatcgat atggaaatgt
    10021 ctttcatcga tcaagaagac attatgcaac tcaatgaaaa acttctttgt accatttgga
    10081 aagaaatcaa aggtatcgaa gtcggcgttt tgccgcgtat gacttatcag gaagccatgg
    10141 atcgttttgg taatgataag cccgacactc gtttcggcat ggagatcaaa gaccttaaag
    10201 ggatcgtcac ggggtgtggt ttcaaagttt tcgacgatgt gattgcccgt aatggaattg
    10261 tcagaggtat tcccgtgcct aaaggcggca gtttttcccg aggacaattc gataagttga
    10321 cggacattgc aaaacgcgcc ggagccaaag gcttggtatg gattaaagcg gaagcggatg
    10381 gcatctattc ttcaccagtt tctaaattct tcacctcaga aaaactcggc gaaatattta
    10441 aagccgttgg agctcaatcg ggagactgtg ctttaattat tgcggatgat tttgatccgg
    10501 cctgcgcggc tctttccaca atgcgtctgc acttgggtaa agagctgaac ctcatcgata
    10561 cttcaaaata taatttcttg tgggtggtgg actttccggc ttttgagtac tcacccgatg
    10621 ataaacgctg ggtatccaga catcatccct tcacttcacc aaaagatgaa ttttcccaag
    10681 accttattga cggcaaggag tcttcttatg gaaatatgct ggcaaaagcc tatgaccttg
    10741 tttgcaatgg ttacgagatg ggtggtggaa gtattcgtat ttatcgtacg gaactccaac
    10801 aagccatgtt ccgcctttta ggtatgggca aggaagagca agaaagtaaa tttggtttct
    10861 tgttagatgc cttgaagtat ggagctcccc cccatggtgg tattgcttgg ggtttagatc
    10921 gccttgtgat gcttctgtgt aatacagatg ctattcgtga agtgattgct ttccctaaaa
    10981 cagctaaagc aagttgttta atgtcagatt gcccgagcga agtcagtcgt gatcaactca
    11041 cggaagttgg gattaagctg acatcactgg cggaaaaggc agtggaagaa gcgaagaaaa
    11101 tactttaagg ttctaagact taaagttgga ggttctgtat gagtcagcct ggaactaaac
    11161 ttttcacgcc cattcgtttg cgggaattga atattaaaaa cagaattttt atgtctgcga
    11221 tgtgccagta ttcagcgatc gatggagttc ctaataactg gcatttagtt catttaggca
    11281 gtcgcgcggt gggtggagct gggcttataa tggtggaggc cacagcggtt cggcctgtgg
    11341 ggcgaatttc tcctgcagat ctgggaatct ggaatgatca tcaaatggaa gcctatcgtc
    11401 ccattgctga ttttttaaaa gtacatggag cgattccagg aattcaattg gctcatgccg
    11461 gaagaaaagc ttcgacagcg gcttcatgga agggcggccg ccccattgag ggtaaacacg
    11521 gcggatgggt gcctgaaggg ccaagcccca tccctttcag tgctgagcat gcggtgccgc
    11581 gagagctcac taaaagtgat attgaagctc tcgttgaaga ttttgcctcg gcggcgaaac
    11641 gcagccttga agccggtttt caggtggtag agattcatgc agcccatggc tatttaatgc
    11701 atgagttttt atctcctctt tcaaaccatc gaaccgatga atatggcggc agcatcgaaa
    11761 accgcatgcg ttttccgtta gaggtggcgg agcgcattcg taagatctgg cctgcgaaat
    11821 ggcccgtgtt tgtgcgaatc tctgccaccg attgggtaga gggcggatgg gatttgcccc
    11881 aaagtaccgt gtttgcaaag caacttaaag acatcgggat tgatttgatt gattgctctt
    11941 ccggtggaaa ctctccgcag gcgcggtttt caccgggacc tggatatcaa gtagccttcg
    12001 ccacggccat tcgcgagcag gcacaaatct tcaccggtgc agtgggggtg atcaccagtg
    12061 gccttcaagc ggaaaaaatt cttcaagaag gtcaggccga cgtcattttt gtgggacgcg
    12121 aatttttgcg cgatccttac tttcctcttc acgccgcacg cgagctcgga gtggatttgc
    12181 catggccacc gcaatacgat cgagcgaaat agaggcgtta ttgtcttcag ctattgaact
    12241 tctccaacga caaagagttt attctcttcg ctgactttaa cccaaggaag ttttaaaaaa
    12301 agtgttaccc aggcagtaag atttttgcag gcggggtttt gggttaaaat ctgtttgtta
    12361 ataataaagc cacgatattt atgtaccatt aaatgctgat gaagtgagtc tggattcgtg
    12421 aaaaggggag cgttggtata ggcagtggcc tctccgtcgc aacccatttt ccctaaaaag
    12481 tcatctttca taagattctc gacgacctct ttattgtaga ggggataggc caaataacct
    12541 gaagtgattt ttttatcatg cagaatttga ttgaagtttt gtgaaggatc ctgggcccag
    12601 gcagaaggga tttggaccac taatgtatct gggggaagat ttctcaatgt ggttaatcct
    12661 gtgataggca ggatgctggt ataatttaag gtcgggaaga accacatatc attcatcaaa
    12721 gggcccaggg caaggggcca cgcgataagc gccacggaga aacatttttt gaaacgattt
    12781 ctacttacat ctccgcttgc tttccattga tccaaaaaca aaaaggctaa gaatataaag
    12841 cagagattgg taatcaccgc caagcgacct ggagtccgag tcacattgag tggaaaatga
    12901 ttgaaaattt ccgaattaaa atccagataa tagaagactt cgttcgctat cctcagttct
    12961 ggtcctaggg ataagataaa ataaagaatg cctatgatta aaatcaaaat gcgcaaaggt
    13021 tctttccaga actttctggt aaaaagggat gccagaacca aaataagaat caggggatcg
    13081 aaactgttat tcctttccag gcttagcttt atcaaagctg gccaccagct atagagaagg
    13141 ttgttttcgc tcggaataag agggcttaaa aggtccaagt tatagagttg tctctgcgaa
    13201 gctaggggga cgactccatt ttccagagta aaagaaatca taggacccca taagccaaag
    13261 aaaataatag aagccaagac taatcccata aaaatattca aaaggcttcg cgagtttgct
    13321 aaatcacggc gcaatttcca aagagtataa agcacgagtg ccaggcttat aaggaaaagc
    13381 ccgggaatat tttgaaatga gccggtataa ctcagaccta aaagaagacc cgcagtaagc
    13441 acattcttaa gattgcgcgg atccagagtc aaaaccgcat agaggacaaa ctgcataccc
    13501 caaatcatag acaaaagagt cagatgttga tgggatcggg agatatagaa tccgcaccac
    13561 caaacgaaca agatataggc gatctgaagc agagatgatt ttataaaccg agtggccact
    13621 aaccaggctg ccaggataat ca
//
LOCUS       DATBTE010000036         2589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2395073, whole genome shotgun sequence.
ACCESSION   DATBTE010000036 DATBTE010000000
VERSION     DATBTE010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2395073"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VIG33_01535"
     CDS             complement(<1..548)
                     /locus_tag="VIG33_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269543.1"
                     /translation="MAVVIHEDTNKEESKLFSDMGIGSTDMGEPSSLRYRVLTLVLAE
                     RYDGAIQELKGFIEQPSEFPDFKERTYRFINHCIDLIYAIKAKRSFPGISSLTRAKQQ
                     ELREKFKEHLRELQFSIKKIEKNQKDLRVSDARSTIYVVRTAWYSVVALVSLWFFLEI
                     SNGLAKTTFYVIDDTMTRGSDW"
     gene            724..1872
                     /locus_tag="VIG33_01540"
     CDS             724..1872
                     /locus_tag="VIG33_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269544.1"
                     /translation="MDFESFQNIRLQNLGFNEEPTPSSPQPQQRHLENTPLPDEMPAA
                     FNYAHLPKEILKSSSVESLISQNEDLVTRLKVALRRLSLIENENQKLSEETQRARLSQ
                     TAVADQVLIFKEKDGLWKKKLQNAEKEKDIQLEKANALQSKVEKLAAEVERYHKYHDR
                     IKTQVKPYIHQLKEYSRSLEEKTQHLSQNNSRAEAQLSDLRHQIMEVSKNSRLQIEIH
                     EKKSQEMIHFYEQQIEAQTAELRHLNEQNKEFEIKAIRLNKALEKQDELENQVIELLR
                     SKEEVKTRLEQETLRLQDKNSELFRLNKRLEIERADLHELALNNEAQIKTFQKEKQDL
                     NEQLDSLRYMWNAKVEEAEKLKSAIQSLERLNVDLSQKVNELRTQQNT"
     gene            complement(1857..2195)
                     /locus_tag="VIG33_01545"
     CDS             complement(1857..2195)
                     /locus_tag="VIG33_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY8269545.1"
                     /translation="MEAKFYQEGDVIVVTLSGRLDIDKTQAFRAACLQSLKGKKVVFC
                     LKNLSFVGSTGIQGFFQVIREFNESKYFSAKVSELKPDFSKLLSFSGSPDLEVCESME
                     KALMSFQVFC"
     gene            2344..>2589
                     /locus_tag="VIG33_01550"
     CDS             2344..>2589
                     /locus_tag="VIG33_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269546.1"
                     /translation="MAIHTIRQRLLFTVIFFLGCIFSEQAFAQSSAKIQGILLNADSM
                     FRDTENETMELEGNVQVVFRTQHIKCDKAKINFRTRQA"
BASE COUNT          864 a          599 c          462 g          664 t
ORIGIN      
        1 aaccaatcac tgccccttgt catggtgtca tcaataacat aaaaagtagt tttagcaagg
       61 ccatttgaaa tttccaaaaa gaaccacagg gaaacaaggg ccacaacaga ataccatgct
      121 gtcctcacca cataaatggt agagcgagcg tcagagaccc gaagatcctt ttgatttttc
      181 tcaatctttt taatggagaa ctgaagttcc ctaagatgtt ctttaaactt ttcacgcagc
      241 tcttgttgct tagcgcgagt caaagaagaa atccccggaa agctccgttt tgctttaatt
      301 gcatagatta aatcaataca atgattgatg aatctataag tgcgctcttt aaaatccgga
      361 aattcagaag gctgttctat aaaccccttc agttcttgga tggctccatc atagcgttca
      421 gccaaaacca aagtaagaac gcgataacgt aaagagcttg gctcccccat atcggtgctt
      481 ccaataccca tgtcactaaa caatttggat tcttctttat ttgtgtcttc atgaataact
      541 acagccatac ccagtccttt tcggctcatc ttccaggacc ttaagcccca cttcgagggt
      601 cccaaaaaaa atctcgcttt aagtctagtt ttcctctaag ccagcctaaa gttttgacac
      661 tttcagcgat tttttgcgaa gctaaactaa ggaccacgcc ttggatctca tggaggagct
      721 tctatggact ttgagtcttt tcaaaatatt agattacaga atctaggttt caacgaagaa
      781 ccaacccctt ctagccctca accccaacag cgtcacctag agaatacccc cctacctgac
      841 gaaatgcctg cggcctttaa ttatgcccat cttccaaagg agatcttaaa atcctccagc
      901 gtggagagcc tcatttcaca aaatgaagac ttggtaacac gccttaaagt ggcacttcgt
      961 cgcctctctc tgattgaaaa tgaaaatcaa aaattgagcg aagagaccca aagggcccgg
     1021 ttatctcaga cggcggttgc agaccaggtt ttgattttca aagaaaaaga tggcctatgg
     1081 aagaaaaaac tgcaaaatgc tgaaaaagag aaagacattc aacttgaaaa agcgaatgcc
     1141 ttgcaaagca aagtcgaaaa attagctgcg gaagtagaaa gatatcataa ataccatgac
     1201 cgtattaaga ctcaagtaaa gccttacatt caccaactca aagaatattc acgcagcctt
     1261 gaagagaaaa ctcaacatct aagccagaac aattctcgtg cagaagcaca actcagcgat
     1321 ctgcgccacc aaatcatgga agtcagtaaa aactctcgcc tgcaaattga gattcatgaa
     1381 aagaagtctc aggaaatgat ccatttttat gagcaacaaa ttgaagctca aacagcggaa
     1441 cttcgacatc tcaatgaaca gaataaagag ttcgaaatca aggccattcg cctcaacaaa
     1501 gcccttgaaa aacaggatga gcttgaaaat caagtgattg aacttctacg ctccaaggaa
     1561 gaagttaaga ctcgtctaga gcaagaaaca ctgcgcctac aagacaagaa ttcagagctt
     1621 ttccgtctca ataaacgcct agaaattgag cgcgccgacc ttcacgagct tgcgcttaat
     1681 aacgaagcac aaattaagac tttccaaaaa gaaaaacagg atctcaacga gcaattagat
     1741 agtttacgct acatgtggaa tgcgaaagtt gaagaggctg aaaaacttaa atcagctatt
     1801 caatccctag aaagattaaa tgtcgatttg agtcaaaaag taaatgaact gaggactcaa
     1861 caaaatactt gaaaactcat cagtgccttt tccatacttt cgcagacttc caagtccgga
     1921 gagccactaa aggataacaa ttttgaaaaa tcaggtttca actcagaaac cttagcacta
     1981 aaatacttac tctcattgaa ttcacgaatg acttgaaaaa aaccttgaat accggttgag
     2041 cccacaaagc tcagattctt caagcaaaag actacttttt tacctttcaa actctgtaag
     2101 catgcggcac ggaaagcctg cgtcttatca atatccaggc gaccacttaa agtaaccact
     2161 atcacatcgc cttcttgata aaactttgct tccataaaaa ccccttttct atgttcttct
     2221 ttttcggtat tcctttactt tgttatagag ggcaatggac tagaaataat ctcgacaatc
     2281 ttcactttgc cccctaagat ttgacttgga ctaacatcta gctaaaacgg atttggcaac
     2341 tatttggcaa ttcatacaat tcggcaacgc cttcttttta cagtgatctt cttcttggga
     2401 tgcatattct ccgagcaagc tttcgctcag tcttcggcga agatccaagg cattttactt
     2461 aatgccgaca gtatgtttcg agacactgaa aatgaaacta tggagcttga aggcaatgtg
     2521 caggttgtct ttcgaactca acacatcaag tgcgacaaag caaagatcaa cttccgcacc
     2581 cgacaagcc
//
LOCUS       DATBTE010000037         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2326698, whole genome shotgun sequence.
ACCESSION   DATBTE010000037 DATBTE010000000
VERSION     DATBTE010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2326698"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1087)
                     /locus_tag="VIG33_01555"
     CDS             complement(179..1087)
                     /locus_tag="VIG33_01555"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEY8269547.1"
                     /translation="MKYLGYIFIFSILVIFAACTENNNQPGYRPSFQSQPLNTQRKEY
                     LFGIHPLYNPQRMLEIFGPLMDILSQEIPEIQFSLEASRNYADYDQKISSRKFHLALP
                     NPYQTIKSFKYGYRVMAKMGDDKDFRGIILVRKDSNIKTLLDLKGKKVNFPAVTALAA
                     SIMPQYYMQTHGLDVKKDIKVGYVGSQESAILSAFNKEVAAGVTWPLPWKTLSQARPE
                     LLKEMIILAETEPLLNNSLIVRDDIEPEIIEKVKNVLIHLHENERGRKILRKLELSKF
                     ENATNGTYKPVQDFMKKYTALLGTVD"
     gene            complement(1456..>1642)
                     /locus_tag="VIG33_01560"
     CDS             complement(1456..>1642)
                     /locus_tag="VIG33_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269548.1"
                     /translation="KNEGKMWKHFFSTQSACEKFLALQQKSYELKNGKLPAEKPAAAE
                     DRDAEDTENEDNSETSE"
BASE COUNT          477 a          298 c          303 g          564 t
ORIGIN      
        1 ctaaagcttt agcaatcgaa agtgtagtct cattgatttg aagatttaga atttcctttt
       61 cttgtttcac tcgatgaaaa acaaaaccac tcatgagaaa tgcatgaacc attgtgacgc
      121 cgacaatgag atgcattcga atagataaat ttttgatcgt atttaaaaag ataaacatct
      181 aatctacagt tccaagtaaa gcagtgtatt tcttcataaa atcttgaaca ggtttataag
      241 tgccgtttgt ggcattttca aattttgaga gttccagttt tcggagaatc tttcttcctc
      301 tttcattttc atggagatgg ataaggacgt tttttacttt ttctataatt tctggttcaa
      361 tatcgtctct gactataagg ctgttgttaa gcaggggttc tgtttcagca agaattatca
      421 tttctttaag aagctcaggc cgtgcttggc tgagagtttt ccagggcaaa ggccaagtaa
      481 cacccgcagc aacttcttta ttaaaagctg ataaaattgc agattcttgc gagccgacat
      541 acccaacttt aatatctttt tttacatcaa gaccatgggt ttgcatataa tactgaggca
      601 tgatggaagc tgcgagtgcc gttacagcag ggaaattcac tttttttcct tttaaatcca
      661 ataaagtttt aatgttgcta tctttacgga ccaaaataat tccgcggaaa tctttatcat
      721 cacccatttt tgccatcact ctatacccat atttaaaaga tttaatggtt tgataagggt
      781 tcggtaaggc aagatgaaat ttgcgcgagg atatcttttg atcgtaatct gcataattgc
      841 gagaagcttc tagggaaaac tgtatttctg gaatttcctg cgacagaatg tccattaatg
      901 gaccaaaaat ctccagcatc cgttgtgggt tgtataaggg gtgtatgccg aataaatatt
      961 cttttctttg cgtattgaga ggttgagatt gaaacgaagg tctgtaacct ggttgattgt
     1021 tattttcagt gcatgcagcg aaaataacaa gaattgaaaa tataaagata tatcctaaat
     1081 atttcacaga ctactttctg aatctaaatc ctttttaagg ttttattttc gctggagttt
     1141 attgaatttc taaaataaac tttttgaagc tctcctgtgt gagattagca aatcactctt
     1201 tatcatatcg aatcaaccag aaaacttcaa attcaatccg atgcagaaaa ttgatgagcc
     1261 tcctagtctt cagtcttgaa aatatcttta ctacttttgt gacagataaa gtggagcgtt
     1321 gggtccttcg catccttttc catagttttt tgcgatttga atacataatt gccggaaata
     1381 agctcggacg gggccgccaa catcaactcc aagggtcttg aaaaatggaa tgtcgctgaa
     1441 gtaagaaatt tcgggttact ctgatgtttc agaattatcc tcattttcag tgtcttccgc
     1501 atccctatcc tcggccgctg caggtttctc tgcgggcaat tttccatttt ttaattcgta
     1561 acttttttgt tgcagcgcta aaaatttctc acaagcactc tgagttgaga aaaaatgttt
     1621 ccacatttta ccttcgtttt ta
//
LOCUS       DATBTE010000038         7029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_209337, whole genome shotgun sequence.
ACCESSION   DATBTE010000038 DATBTE010000000
VERSION     DATBTE010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209337"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VIG33_01565"
     CDS             complement(<1..203)
                     /locus_tag="VIG33_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269549.1"
                     /translation="MGSIFLLIIPQLVFAESIWIHAIGIDELNPTQNYPTFYRSAADF
                     NEKCDQSGKSAQCHLFINNEPQYR"
     gene            complement(437..949)
                     /locus_tag="VIG33_01570"
     CDS             complement(437..949)
                     /locus_tag="VIG33_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269550.1"
                     /translation="MRAMLTIFGLLFVSSTYFTSTSFAEEIVKTCSIKMKMPGDNRVI
                     PTTFQIVKSGNVLIAKTIQTVNGNSGELPNESASIAEFSVRAGLTANTPNMNEAENLI
                     QGTEGFLNNPDIGSSFSVGLDIKSVRSAKVYEIGKFTHMGGTVIIEAKDKDGKEMGSF
                     VTGFMPFACE"
     gene            complement(1230..1559)
                     /locus_tag="VIG33_01575"
     CDS             complement(1230..1559)
                     /locus_tag="VIG33_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269551.1"
                     /translation="MRLFQGSGCEYGIDWIIKELLLLQENLETVDLDEAFENFVRESY
                     LEETTVGWMTLDTVSVMKEMDPVSWSCAQSDWESQLADEDLISFDNGFTYYWRSDLEG
                     FIEEQGL"
     gene            complement(1630..1911)
                     /locus_tag="VIG33_01580"
     CDS             complement(1630..1911)
                     /locus_tag="VIG33_01580"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HEY8269552.1"
                     /translation="MKNKLKNSKKNRVENIDDPLDGDLSDFIAESGWQFAKFELREKK
                     DKVLSLRLSDRLLTELKKQAKAMGLDTQKFVRIILESTVRKKAEHRRAP"
     gene            complement(1877..2200)
                     /locus_tag="VIG33_01585"
     CDS             complement(1877..2200)
                     /locus_tag="VIG33_01585"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEY8269553.1"
                     /translation="MKYGNLEYDGFDWDEGNTAKIESRVSIKAVEEFFEMKIIFKEDT
                     RHSLSEERFIAMGHTKSPKRCLFVAYTIRIKGNEILIRPISARFTHKKEEEAYEEQVK
                     KLQKK"
     gene            complement(2289..3320)
                     /locus_tag="VIG33_01590"
     CDS             complement(2289..3320)
                     /locus_tag="VIG33_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8269554.1"
                     /translation="MSQTTSETMSDNKPLPFSSALPAPAFPLQSRNPLWSAELQSLQG
                     VAQTDFPVLLLGPSGTGKDIIAQAIHKSSRRAQAAFVSVNCSALTETLIESELFGHVK
                     GAFTGAVNDRKGAFESARFGTLFLDEIGDLPFNLQAKLLRALENNEIRPLGSDRITKT
                     NIRVIAATHQNLSQKISDGQFRSDLFFRLNVVSIYTPSLKERIEDFEDLLYAFAKQMR
                     VRFSFDAVQKLKMYSWPGNIRELRNLVSRAAALFPHQQIDLERTERLLRGSTVFPSSS
                     EGPSQTRMSVIKEVEKTMILKKLQENRGNQRKTAQDLGMPKSTLNDRLRQYNIDYKVY
                     KIRPDSVYG"
     gene            3485..4894
                     /locus_tag="VIG33_01595"
     CDS             3485..4894
                     /locus_tag="VIG33_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY8269555.1"
                     /translation="MRLATKEEIYEIDKLASQDYGLSSSRLIEKAGAALAERFEKIFY
                     QQNWHKGWKIGVWCGTGNNGADGRVMAHLLREKGFSVEVLQGASWRPEDFEIIVDAFF
                     GVGLNRPIEGALKEQILRLNKSERKVLAVDIPSGLDANTGMVLGIAVKAHWTLTIAPA
                     KPGLFLQQGPTQSGRIQSLDIGFPRELVSKVANTVYLVGKASARKLLPSRTSTSNKTH
                     FGHLLIIAGSAGMAGAALLAAEAAARMGCGYVTLCSPSSRIFDKARPDFLRLSMDRFF
                     QNDLKKYTAVVVGPGLGVSQQTSQILEYLLKQHGKVLVDADALTVLSQNPSWQLPENW
                     LLTPHAGELSRILGIPGKNLEEDRLQSVQKAQKNKTCLVLLKGFRTVLGCPQKTYIIG
                     SGNVALAKAGSGDVLSGFIGSLMAQNLATDKAAALGAYLHGWIADDWLRRGHSNRTLM
                     ASDLPELLDASLRSLKKSP"
     gene            4913..5491
                     /gene="yihA"
                     /locus_tag="VIG33_01600"
     CDS             4913..5491
                     /gene="yihA"
                     /locus_tag="VIG33_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY8269556.1"
                     /translation="MSGNIKFIKSAVLPKDYPQTGKKEIAIAGRSNAGKSSFLNALGN
                     TKIAKVSNTPGKTRLLNFFSRGDSYILVDMPGYGFASRSGNEMREWHTMIENYLSMRE
                     ELAGLVLVMDSRREWTEDEELLKAFTDSRGLPLAVVLTKSDKLNRNDIQKALLKIKKD
                     AATEAVFMTSALKKDGQEAVEEYVFENWIKNK"
     gene            5488..6237
                     /gene="kdsB"
                     /locus_tag="VIG33_01605"
     CDS             5488..6237
                     /gene="kdsB"
                     /locus_tag="VIG33_01605"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEY8269557.1"
                     /translation="MKFIGVIPARYASTRFPAKPLALLQGKPMIQWVVEGARKSQLLS
                     EVIVATDHEEIRKAVEAIGGKVVMTDSNLPSGTDRIQAAVRDLDFDVVINIQGDEPLV
                     NGELIDSLAGLFRKGSGIDMATLAHPISAEELESVNAVKVVLNRQDEALYFSRYAIPY
                     SRVSGRQALSLNGSLNGCLKHIGMYAYTKKFLKTFCEAAPAAIEVAESLEQLRALYLG
                     AKIKVLRVKEASLGVDTPEDLVKLEKLLGGN"
     gene            6237..>7029
                     /locus_tag="VIG33_01610"
     CDS             6237..>7029
                     /locus_tag="VIG33_01610"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY8269558.1"
                     /translation="MAVKTTKKKSAVRSVVKSTAKSTKKRLQQKFIFVTGGVVSSIGK
                     GLTAASLGSLLEARGIRVTIMKFDPYLNVDPGTMSPLQHGEVYVTEDGAETDLDLGHY
                     ERFTSAVMSRSNSVSTGQIYDTVLNRERRGDYLGGTVQVIPHITDEIKARAQEAAQGS
                     EVLIVEIGGTVGDIEGQPFLEAIRQMRSELGENNSVLMHVTYVPYIAAAGELKSKPTQ
                     HSVKELRMIGLQPDFLVCRSEKSLDDGIKKKIGLFCSVDPKNVIAA"
BASE COUNT         1880 a         1482 c         1698 g         1969 t
ORIGIN      
        1 cgatactgag gttcgttatt aataaaaaga tggcattgag cagactttcc tgattggtca
       61 catttttcgt taaagtcagc agcacttcgg taaaaagttg ggtaattttg tgttggatta
      121 agttcgtcga ttccgattgc atgaatccat atgctttcag caaacaccaa ttgcggaatt
      181 attagtaaaa atattgaacc cattggttca gaatatcctt cccaagaata caaacaagtc
      241 tagtgagtac tcaaaacaat gggctctgaa agatatagac gaaagtccat gcttacgtga
      301 tttccagggc gatattcaaa attaatcagc tatgattgaa tcccatcaag acgggcaatg
      361 atgattaaaa atttatcgcc catcaaggac tacttcaatg cataagcagt ccatgaaaag
      421 gtaattaata aagtgattat tcacaggcaa atggcataaa tccagtgaca aatgatccca
      481 tctcttttcc gtccttatct ttagcttcga taattaccgt gccacccatg tgggtaaact
      541 taccgatctc gtataccttg gcactcctaa cagacttaat atctaatcca acgctaaatg
      601 aactcccaat gtctgggtta tttaagaacc cctctgttcc ctgaataaga ttctctgctt
      661 cgttcatgtt tggtgtattc gctgttagac cagcgcgaac cgaaaattca gcaatacttg
      721 ctgactcatt tggtaactct ccactattgc catttaccgt ttgtattgtt ttagcaatta
      781 aaacatttcc gcttttaaca atttgaaatg tagtaggtat tacacgatta tcgccaggca
      841 ttttcatttt aatggaacaa gtcttcacaa tctcttcagc aaatgaagtg gaagtaaaat
      901 acgtcgatga aacaaacaag aggccaaaaa ttgtcaacat tgctctcatg gaatctccta
      961 tttttgatat aaaaatttaa actaccgtta aagcaatacg acagcaaacc atgtgccatt
     1021 gaaaatggca ttcagcatcc aaaatcattt atgaacaaaa tcaaacctta aacagcctaa
     1081 aaacgaatac aatcgtaccg ggcacttgaa caatcgcgcg ccgcatgcag gcggcttaaa
     1141 acgcaagcgt ctggtacaga atgcagagat cgaaagcaac ggtcttcttg gactgtcagc
     1201 gggccccctg cggatgccaa ggcctcgggc tagaggcctt gctcctcgat aaagccctca
     1261 aggtccgatc tccagtaata agtgaacccg ttgtcgaaag aaatcaggtc ctcatcggcc
     1321 agctgagatt cccaatcaga ttgagcgcag ctccaggaga ccgggtccat ttccttcatg
     1381 acggatactg tgtcaagagt catccagccg accgtggtct cctcgagata tgactctcgc
     1441 acgaagttct caaaggcctc atccagatca acagtctcaa gattttcctg gagaaggaga
     1501 agttctttga taatccagtc gattccatat tcgcagccag agccttgaaa taagcgcatt
     1561 aaatcgtcgg acccgcaagt tttgcatctg ccggatgccg cttattcaat tctagccata
     1621 gactgtcatt cagggagctc ttcggtgttc agcttttttt ctaacagtgc tttcgagaat
     1681 aatcctaacg aatttttgag tatcaaggcc catcgctttg gcttgttttt ttaactcggt
     1741 cagtagccta tcgctaagtc taaggcttaa aaccttatcc tttttttccc ggagctcaaa
     1801 cttggcaaat tgccacccac tttcagcaat gaagtcactt aaatctccat ccaatgggtc
     1861 gtcaatattt tcaaccctat tttttttgga gttttttaac ttgttcttca taagcctcct
     1921 cttctttttt atgagtaaat ctggcgctaa tgggtcgaat taaaatttcg ttccctttta
     1981 ttcttatcgt gtaggccaca aataaacatc ttttcggact tttggtatgt cccattgcta
     2041 taaaccgttc ttcacttaaa gagtgtctgg tatcctcttt aaaaatgatt ttcatttcaa
     2101 agaattcttc tactgctttt atggacaccc tggactcaat ttttgctgta ttgccctcgt
     2161 cccaatcaaa gccatcgtat tctaagttgc catacttcac tgcttatatc ctatcacaaa
     2221 tggactacca atgtattaca tttgtatgta tataatttta ctgatctatc tagcttaatt
     2281 tttctttcct aaccataaac cgagtccggt cttatcttgt agaccttgta gtcgatatta
     2341 tactggcgaa ggcggtcatt cagggtgctc tttggcattc ctaaatcttg cgctgtcttt
     2401 ctttggttgc ctctgttttc ctgtagtttt ttaaggatca tggttttttc cacttcttta
     2461 atgacggaca ttcttgtttg cgaggggccc tcactgcttg aaggaaaaac cgtggagcct
     2521 ctcagcaggc gttccgtgcg ttcgagatca atttgttggt gggggaaaag ggccgccgcc
     2581 cgagagacca aattgcgcag ctcacggatg tttccgggcc aggagtacat ttttagtttt
     2641 tgaacggcat caaaggaaaa ccgcactctc atttgcttgg cgaaagcgta aagtaaatct
     2701 tcaaagtctt cgatcctttc tttcagggag ggagtgtaaa tggagacgac gtttaagcga
     2761 aaaaaaagat ccgaacgaaa ttggccgtcg ctaatttttt gagagagatt ttgatgggta
     2821 gccgcgatca cgcggatatt ggttttagtg atgcggtcac tgccgagcgg acgaatctca
     2881 ttgttttcta gtgcacgtaa gagcttggct tgtaaattaa aagggagatc gccaatttca
     2941 tcgaggaaaa gggtgccgaa gcgggcgctt tcaaaagctc ctttgcgatc gttcacggct
     3001 ccggtgaagg cgcctttgac atggccaaaa agttcgctct caatgagagt ttccgtgagg
     3061 gcgctgcagt tgacgctgac gaaagcggct tgggctcggc gtgaactttt atgaattgcc
     3121 tgggcgataa tatcttttcc agtgccggag gggcctaaaa gtaaaactgg aaagtcggtt
     3181 tgggcgactc cttgcaaact ctgcagttcg gcactccata aagggttgcg actttgtagc
     3241 ggaaacgccg gagccggaag ggcagaggag aagggaagag gcttattatc agacattgtt
     3301 tctgaggttg tttgggacat atgagactcc tatgttgtga ctttgagcat tacgcgaaag
     3361 ggtctttcca aaaattggac caactctaag ttgaataagt tgagtctcag catttgccgg
     3421 acaaacttgt cttagtttta agaagttggt tctcagattc gagaatttcc ggcaagatcg
     3481 ggctatgaga ctggcgacaa aagaagaaat ttacgagata gataaactcg cgagccaaga
     3541 ctacgggctg agttcttcgc gacttattga aaaagcgggt gcggctctgg cggagcgttt
     3601 cgaaaaaata ttttaccaac aaaattggca taaaggatgg aaaataggag tctggtgcgg
     3661 caccggaaat aacggagccg atggccgggt gatggctcat cttctgcgcg aaaaaggttt
     3721 ttcagtggag gttttgcagg gggcttcgtg gcgacctgaa gatttcgaaa ttattgtcga
     3781 tgcctttttt ggggtgggcc tcaatcgtcc gatagagggg gctcttaaag agcaaattct
     3841 gagactcaat aaatctgaaa gaaaagttct cgcagtggat attccgagtg gtcttgatgc
     3901 caataccgga atggtgctgg gaattgcggt gaaagctcat tggactttga cgattgctcc
     3961 cgccaagccc ggattatttt tgcagcaggg accgacacag agtggtcgca ttcaaagttt
     4021 agatattgga tttcctcgcg agcttgtgag taaagttgcc aacactgttt atctagtggg
     4081 aaaagcaagc gctagaaaat tgcttccttc cagaacttcg acttcaaata aaactcactt
     4141 tgggcatctt ttaattatcg ctgggtcggc aggtatggcc ggtgcggcct tgctggcggc
     4201 ggaggctgcg gcgcggatgg gctgcggtta tgtgactttg tgttctccga gttctcgaat
     4261 ttttgacaaa gctcgtcccg attttcttcg tctttctatg gatcgttttt tccaaaatga
     4321 tttgaaaaaa tacacagcgg tagtggtggg acccgggctc ggagtgagtc agcagacttc
     4381 gcagattctc gaatatttgt tgaaacagca tggaaaagtt ttggtggatg cggatgcctt
     4441 gaccgtgctt tcgcaaaatc cttcgtggca gttgcctgaa aattggcttt tgactccgca
     4501 tgcaggggag ttatcgcgca ttttgggaat tcccggcaaa aaccttgagg aagatcgcct
     4561 gcaaagcgtg caaaaggccc aaaaaaacaa aacttgcttg gttttattga aaggctttcg
     4621 cacagtgcta ggttgtccgc aaaaaactta cattatcggt tcagggaatg tagcccttgc
     4681 aaaagccgga agtggcgacg ttttgagtgg atttattggc tctttgatgg cgcaaaacct
     4741 tgccacggac aaagcagcgg ctttgggggc atatctgcat gggtggattg ccgatgactg
     4801 gctccgtcga gggcactcaa atcgcacttt gatggcttcg gatctgccgg aattgcttga
     4861 tgcaagcctc aggagtttga aaaagagtcc ttaatagatt aagctcttcg ccatgtctgg
     4921 aaatatcaaa tttattaaga gtgcggtcct gcctaaggat tatccacaaa ctggcaaaaa
     4981 agagatcgct attgcgggca gatctaatgc cggcaagagt tcttttttga atgcccttgg
     5041 caacaccaag atcgctaaag tcagcaacac gccagggaaa acccgtttgt tgaatttttt
     5101 cagtcgtggc gatagttata ttttggtgga tatgccgggc tatggctttg cctctcgttc
     5161 cggaaacgaa atgcgcgaat ggcataccat gatcgaaaac tatctgagca tgcgggaaga
     5221 gctggccggc ttggtccttg tcatggattc gcgtcgtgag tggaccgaag atgaagagtt
     5281 gctgaaagct ttcaccgact ctcgggggtt gcctttggcg gtggtattaa ccaaatcgga
     5341 taaactcaat cgcaatgata tacaaaaagc ccttcttaaa attaaaaagg acgctgccac
     5401 ggaagccgtg tttatgactt ccgctttgaa aaaagacggc caagaagctg tggaagagta
     5461 tgtttttgaa aattggataa agaataaatg aaatttatag gcgttattcc cgctcgttac
     5521 gcttccactc gattccccgc taaacctctt gcactccttc aaggtaaacc catgattcag
     5581 tgggtggtcg agggcgctcg taagagtcag cttctatcag aggtgatcgt ggccacggat
     5641 cacgaagaaa tccgtaaagc agtggaggcc atcggtggca aggtggttat gactgactca
     5701 aatttgccct cgggaacaga tcggattcag gccgccgtga gagatttgga tttcgatgtc
     5761 gtgatcaata ttcaaggtga cgagcccttg gtgaatggag agctcataga tagcttggcc
     5821 ggactttttc gcaaagggag cggcatcgat atggccactt tggctcatcc gatttctgca
     5881 gaggagttgg aatccgtgaa tgcggtaaaa gtggttttga atcgtcagga tgaagctctt
     5941 tacttcagcc gctatgcgat tccttattct cgagtctctg gtcgacaggc cttgagcttg
     6001 aatggatctt tgaatgggtg tctcaaacac attggtatgt atgcctatac aaagaaattc
     6061 ttaaaaacat tttgtgaagc cgcaccggcg gctatcgagg ttgctgagag cttggaacaa
     6121 ctcagagctc tttatttagg tgcaaaaatt aaagtcttac gtgtgaaaga agcaagcctg
     6181 ggagtggata caccagagga tctggtgaag cttgaaaaat tattaggggg aaactaatgg
     6241 ctgttaaaac tacgaaaaaa aaatcagcgg tcaggtcggt tgtaaaatca acagcaaagt
     6301 ccaccaagaa aagacttcag cagaagttca ttttcgttac gggcggtgtg gtttcttcta
     6361 ttggcaaagg ccttacggct gcgagtttag gttccttgtt ggaagctcgc ggtattcgcg
     6421 tgacgatcat gaagttcgat ccttacctga atgtcgatcc gggcacgatg tcgcctttgc
     6481 aacacggtga agtttatgtc actgaagatg gggctgaaac agatctggat ttaggtcact
     6541 atgagcgttt caccagcgcg gtgatgtctc gttcaaatag cgtctccacg ggacaaattt
     6601 acgatacagt tttaaaccgt gaacgtcgtg gtgactattt gggtggcaca gtgcaagtca
     6661 taccacatat caccgatgaa attaaagctc gggcccaaga ggcggcacag ggatcggaag
     6721 ttctcatcgt ggaaatcggc ggaactgtcg gagatatcga agggcagccg ttcttggaag
     6781 ccatccgtca gatgcgcagt gaactcggag aaaataattc cgtgttgatg cacgtgactt
     6841 atgttcccta tatcgcggcg gcaggggagt tgaagagcaa acccactcag cattccgtga
     6901 aagagttgcg catgattggt ttgcaacctg actttttggt gtgccggagt gaaaaatctc
     6961 tggatgatgg gatcaagaaa aagattggcc tcttttgcag cgtagaccct aaaaatgtta
     7021 tcgcggccc
//
LOCUS       DATBTE010000039         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_482272, whole genome shotgun sequence.
ACCESSION   DATBTE010000039 DATBTE010000000
VERSION     DATBTE010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482272"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1177
                     /locus_tag="VIG33_01615"
     CDS             74..1177
                     /locus_tag="VIG33_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY8269559.1"
                     /translation="MNQFDRTFEVGKLYCDRGDFRAATFHLKEAAAGYFSDKDFSGYL
                     KSMNLLLRIYAEMEMFKEINETKEKLQDLVLKEGFELNSKTYYTLAVCADYKQQMETA
                     LDYLQKALSIALATDNKEDICHAIFGLASVYAHPTVGRYTDALKEIYNLEVFFQVYNL
                     PEIKVSSQMLNANILRRLKKYEEAIAVLWRAYDEIKSTKNFNSIINLQVQLGECYLEM
                     GDKDMARLYLDLAGRMIDPENMVRMARIWKDLSSQLGGEQQNGFDLVFDEHNHAVVEK
                     KLGRIDFKNQFILLDLLRLFMQNQGVAYSKEYLVENVWKQAYDPVIHDNKIYVTIKRL
                     RKLIEPDYEKPKYIFRAKNGYYMNKSARVSFDR"
     gene            1194..>1592
                     /locus_tag="VIG33_01620"
     CDS             1194..>1592
                     /locus_tag="VIG33_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269560.1"
                     /translation="MVRTIKFFQMLLILGSICVGTHSRAEVAQVTRGPGDDTHPWPWG
                     FEVKFPWNEVQGIWRAEDNGKAVYFSFRRVEEKRVKIKQIDFATCSVIGTGQGLERDK
                     TLVAQIITNRSVEPYNLTIYAFNEEDSHKPP"
BASE COUNT          478 a          301 c          354 g          459 t
ORIGIN      
        1 cagattgacg tattgttgag aatctgtcaa aaaatgttct tgatttgatt attttcaaac
       61 ggggatccgc ataatgaatc aatttgatcg cacatttgaa gtgggtaagc tttattgcga
      121 tcgcggcgat ttcagagccg caacttttca tcttaaagaa gctgcagcag gttatttttc
      181 cgacaaagat ttttccggct atttaaaatc aatgaatctt cttcttagaa tttatgctga
      241 gatggagatg tttaaagaaa ttaatgagac caaagaaaag cttcaagact tggtactcaa
      301 agaaggcttc gaacttaatt ctaaaactta ttacaccctc gctgtttgtg cagattataa
      361 gcagcaaatg gaaacagccc tggattatct ccaaaaagct ctttctatcg ctctagcgac
      421 agacaacaaa gaagacatct gccatgccat tttcggtctt gcttccgttt acgctcaccc
      481 tacagtaggt cgttatacag acgcccttaa agagatttac aatctcgaag tgtttttcca
      541 agtttacaac cttcctgaaa ttaaagtttc aagccaaatg cttaatgcca atattctccg
      601 tcggcttaaa aaatatgaag aagccatcgc agttttgtgg cgggcttacg atgagatcaa
      661 atcaacgaaa aattttaata gcatcattaa tctgcaagtc caactaggcg agtgttatct
      721 cgagatgggc gataaagaca tggctcgcct ttatttagat ctggcgggcc ggatgattga
      781 tcctgagaat atggtccgga tggcaagaat ctggaaagac ctctccagcc aactcggcgg
      841 cgaacaacaa aatggctttg accttgtttt cgacgaacat aaccatgcag tggtggaaaa
      901 gaaattgggc cgtatcgact ttaagaatca atttattctt ttagaccttt tgagactgtt
      961 tatgcagaat caaggggttg cgtattcgaa agaatacttg gtcgagaacg tttggaagca
     1021 agcgtacgat ccggtgattc acgataataa aatttatgtc actattaagc gtcttagaaa
     1081 attaattgag cctgattacg aaaagcccaa gtatattttc cgggcaaaga atggttacta
     1141 tatgaataag agcgctagag tttcatttga tcgctaggtg agaggggctc ctaatggttc
     1201 gcacaattaa gtttttccag atgttgttaa tccttggtag tatttgcgtt ggcacgcatt
     1261 cgcgtgccga agttgctcaa gtaacacggg gacctggcga tgatacccat ccctggcctt
     1321 ggggctttga agttaagttt ccatggaatg aggttcaggg tatttggaga gctgaagata
     1381 atggcaaggc cgtctacttt agtttccgta gggttgaaga aaaacgcgta aagattaagc
     1441 aaattgattt tgcaacctgc agtgtaattg gaaccgggca aggtctggaa agagataaga
     1501 ccttagtggc tcaaattatt accaatcgtt cagtagagcc ctataatctg acgatctatg
     1561 cgtttaatga agaggattcg cacaaaccgc ca
//
LOCUS       DATBTE010000040         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2121766, whole genome shotgun sequence.
ACCESSION   DATBTE010000040 DATBTE010000000
VERSION     DATBTE010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2121766"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /gene="rsfS"
                     /locus_tag="VIG33_01625"
     CDS             <1..523
                     /gene="rsfS"
                     /locus_tag="VIG33_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY8269561.1"
                     /translation="GRNIQFIRLQDIEVSASEVRKFLRTGRPVEKVLPLSVESYIRAH
                     ELYRASGDRIGDFEKFAAFCGKVLFEKKAIQVRGFDLTEIAAPTEYTLIASGTSTRHA
                     VSLAESVVQAVKEEYNVLPLSVEGISEGRWVLVDYGSLIVHIFYDFVRQEYSLEKLWQ
                     DGKDLNLKDSSPS"
     gene            526..996
                     /locus_tag="VIG33_01630"
     CDS             526..996
                     /locus_tag="VIG33_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEY8269562.1"
                     /translation="MKFVLYNLATAKETWADEAAKLYTQKISYFNPFEIQSLKPKKSS
                     REEATQKKKKESELLLSVLAKDDFIVLFDERGKTFNSIEFSKKVENILSSSKRRGVFI
                     IGGAYGVSDEVRARAQLVVTLSPMVMNHLLAQTVALEQIYRAFTILKNTPYHNA"
     gene            1024..1716
                     /locus_tag="VIG33_01635"
     CDS             1024..1716
                     /locus_tag="VIG33_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269563.1"
                     /translation="MYFSKNIKWFGLQGCLYCGSLLRYSDGLCDVCSKDLWSWQSKSG
                     GLFVQQIRKLEVFSLFQWIPGRQEVLSRVIYALKGRNGEEFWKTFAEEFQKRRISENV
                     LGLKKPLLFIPSPSKSQSRDHAQLFAKALLEAGGGDIYNCLLRENSINQQKKKSRNQR
                     QRVSLGWAENFSKHDFPKISSGKHVIFVDDVVTTGFTARAAWRTLGKPRDFAVWTLAQ
                     RGLSCGASCSLL"
     gene            1724..2407
                     /locus_tag="VIG33_01640"
     CDS             1724..2407
                     /locus_tag="VIG33_01640"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEY8269564.1"
                     /translation="MFKLHILFCICLTFLTSFSLSAQTKTKGKKQDTAITVLKEVGKK
                     YRNSKLVKIETHKKVTSELLGKTTTYDGIIYLAQGKFRWQNETPEHSLLLFDGEIIWN
                     VQYPPKDLGGPVQVAKAKVDKNTKSQILISTLIGKEPIEKNFKVVREKAEKNILSIEL
                     EPRKSDVRVKGLTLNIEVNSKLIKNISFKDDVNNTTEIEMKKTEFLSKENKELFKYKI
                     PKDAQVTNL"
BASE COUNT          783 a          451 c          545 g          705 t
ORIGIN      
        1 aggacgaaat attcaattta ttcgcctgca agacattgag gtttcagcaa gtgaagtccg
       61 taagtttctg cgtacaggac gtcctgtaga aaaagtttta cccttatctg ttgaatctta
      121 tatccgcgca catgaactct atcgtgctag cggcgatcgt attggcgact tcgagaagtt
      181 tgcggccttt tgtggaaaag ttctttttga aaagaaagct attcaagtcc gcggcttcga
      241 tttgactgaa attgcggcac ccacggagta tacattaatc gcttcaggta cgagcactcg
      301 tcatgcagtt tcactagccg aaagtgtggt tcaggctgta aaggaagagt acaacgttct
      361 tcctttgagt gtggagggca tcagcgaggg acgttgggtt ctagttgatt atggatcgct
      421 aattgtccat atattttatg attttgtccg ccaagagtat tctctcgaga aactttggca
      481 ggatggaaaa gatcttaatt tgaaagattc cagcccttcc taattatgaa atttgtattg
      541 tacaaccttg ccacagctaa agagacctgg gccgatgagg cggcaaagtt gtacactcaa
      601 aagatctctt attttaatcc ctttgagatc cagtcactaa aacctaaaaa atcttctcgg
      661 gaagaggcga cacagaaaaa gaaaaaagaa tcagaattgc ttttatcagt tctcgctaag
      721 gatgatttca ttgttctttt tgatgagcga ggaaaaactt ttaattcaat agagttttcc
      781 aaaaaagtgg aaaatatctt atcaagctct aagagacgcg gagtttttat tattggcgga
      841 gcctacgggg tgagcgacga agtccgagcc agagcacaac tggtagtgac gctttcaccc
      901 atggtaatga atcatttgct cgcacaaaca gtggcacttg aacaaattta tcgtgctttc
      961 acgattctta aaaatactcc ttatcacaat gcctaggcct attttttgct gagagacttc
     1021 agcatgtact tttcaaaaaa tataaagtgg tttgggcttc aggggtgttt gtattgcgga
     1081 tctcttcttc gttattcgga cggactgtgt gatgtgtgtt caaaagactt gtggtcgtgg
     1141 caaagtaaaa gtggtggact ttttgttcag cagattcgta agctcgaagt attttctttg
     1201 ttccagtgga taccaggccg tcaagaagtc ctgtcgcgag tcatatatgc cctgaaggga
     1261 agaaatggag aggagttttg gaaaactttt gcagaagagt tccaaaaacg aagaatttct
     1321 gaaaatgtct taggtttaaa aaaacctctt ctttttattc ccagcccttc gaaatcacag
     1381 tcgagggatc atgcgcaatt atttgcaaag gctcttctag aagcaggagg aggagatatt
     1441 tacaattgtt tattaagaga gaactcaata aaccagcaga aaaaaaagtc tcgaaatcag
     1501 cggcaaagag taagcttagg ctgggctgaa aatttttcaa agcatgattt ccctaaaata
     1561 agctctggaa agcacgtgat attcgtggat gatgtagtta ctaccggttt tacggcaagg
     1621 gccgcatgga ggaccctggg gaaacctagg gattttgctg tttggactct tgcgcagagg
     1681 ggtctctctt gcggagcatc ctgctctttg ctataagcta cgaatgttta aactccatat
     1741 tttattctgc atttgcctga cgtttttaac ttctttctct ttgagtgctc aaacaaagac
     1801 gaaaggaaaa aaacaagaca ccgccattac ggttcttaaa gaagttggca aaaaatatcg
     1861 caacagcaag cttgtaaaaa ttgagaccca taaaaaagtg acttccgagc ttcttggtaa
     1921 aactacaact tacgatggta tcatttattt ggcgcaagga aagtttcgtt ggcaaaatga
     1981 gactccagaa cacagtcttt tgcttttcga tggagagata atttggaatg ttcaatatcc
     2041 accgaaagac ttgggtggcc ctgtccaagt ggcaaaagca aaagtggata aaaacacaaa
     2101 atcgcagatt ctgatttcta ctttgattgg gaaagagcct attgaaaaaa acttcaaagt
     2161 tgttcgggaa aaagctgaaa aaaatatctt aagtattgaa ctggagccac ggaagagcga
     2221 cgttcgagtc aaaggcctca ctttaaatat agaagtgaat tccaagttaa ttaaaaatat
     2281 ttctttcaaa gatgatgtca ataacacgac tgaaattgaa atgaaaaaga ctgaattcct
     2341 atccaaagaa aacaaagaac tctttaaata taaaattccg aaagatgctc aggtaacaaa
     2401 tctatgaagc aggaaacatc aaaagctact aaaaaagttc attttatcag cctaggctgt
     2461 cctaagaatc tggtggatag cgaa
//
LOCUS       DATBTE010000041         2576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_619114, whole genome shotgun sequence.
ACCESSION   DATBTE010000041 DATBTE010000000
VERSION     DATBTE010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619114"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..749
                     /gene="rplU"
                     /locus_tag="VIG33_01645"
     CDS             156..749
                     /gene="rplU"
                     /locus_tag="VIG33_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_038450368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY8269565.1"
                     /translation="MYAIIRTGGKQYKVQAGDVLQVEKLEQDLGKEFDISEVLMIGGD
                     AAPHIGQPLVKDAKVTVVVTKQARTRKVIVFKKKRRQGYRRFNTHKQDFTELFIKAIT
                     SPDGKIIKAEATPNVVDMAAVRTQRIADKQAARKARAEAAVGSGKAATETLKQEAKKL
                     VKKKVAKKAAAKKATKKPAVKKAAAKKKVAKKTSKKA"
     gene            781..1041
                     /gene="rpmA"
                     /locus_tag="VIG33_01650"
     CDS             781..1041
                     /gene="rpmA"
                     /locus_tag="VIG33_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY8269566.1"
                     /translation="MASKKAGGSTKNGRDSQSKRLGVKRFGGQEVLPGTIIIRQRGTK
                     FHVGNNVKIGRDHTIYSVIEGLVKFERYSKERFKISVYPKTA"
     gene            1158..2189
                     /gene="obgE"
                     /locus_tag="VIG33_01655"
     CDS             1158..2189
                     /gene="obgE"
                     /locus_tag="VIG33_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY8269567.1"
                     /translation="MKFIDEVTVTVSSGHGGAGAVSFRRESMAPRGGPDGGDGGKGGD
                     VILRASKHLNSLVDIRNNKKYPAPDGQPGSGSQCSGHNGEDFVLVIPEGTLVKNMDGE
                     VLVDMTGIEQYTLLKGGRGGKGNTFFKTATNQAPEYAQPGEEGQQLEIRLELKLIADV
                     GIIGFPNAGKSTLISRISAAKPKIADYPFTTLTPNLGVVKAADFKSFVVADIPGLVKG
                     AHQGVGLGIQFLKHIERTRFFIHLIDASGMSGRDPINDYEDINNELKMYDQNNQEKEG
                     FFSLSDREQFVVLNKIDTLSEDQLLKLKSSFKKKTGVEPLAISAVTGKNIKELITDLT
                     RKIFQKDAV"
     gene            2186..>2576
                     /gene="nadD"
                     /locus_tag="VIG33_01660"
     CDS             2186..>2576
                     /gene="nadD"
                     /locus_tag="VIG33_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEY8269568.1"
                     /translation="MRIGIFGGSFNPPHMGHLNSLVTVQKKAGLDKIHVVPAAQNPLK
                     TQVAGPTNEQRLELVKLALNSYGNQFFVDDQEIRRGGLSYTIDTVMNLRSKVGSEDLF
                     LIIGADQFEEFSQWKDYQKILTEANLIV"
BASE COUNT          819 a          476 c          589 g          692 t
ORIGIN      
        1 atctggcttc tgcgaaaaag gtttgctttc aaaggttccg cgggctaacc tcatcatctt
       61 ccggaatctg cctaaaaaat aagatctaat cagttgacaa agactggtgc aaggtggcat
      121 ttaagcaagg cttatttcgg gtagctggag gttacatgta cgcaattatt cgtaccggcg
      181 ggaaacaata taaagttcag gctggtgatg ttcttcaagt agaaaaactt gaacaagatc
      241 ttggaaaaga atttgatatc agcgaagttc taatgattgg cggagatgct gctcctcaca
      301 ttggtcaacc tcttgttaaa gatgcaaaag taaccgtcgt tgttactaaa caagcacgta
      361 ctcgcaaagt gattgttttt aagaaaaaac gtcgtcaggg atatcgtcgc ttcaacactc
      421 ataagcaaga cttcaccgag cttttcatta aagcaatcac ttctccagat ggaaaaataa
      481 tcaaagctga ggctactcct aatgtggtag atatggcagc tgttcgtact caaagaattg
      541 ctgataaaca agcagctcgt aaagcacgtg cagaggcagc ggtcggtagc ggtaaagcag
      601 ctactgaaac tttgaaacaa gaagcaaaaa aacttgttaa gaaaaaagta gcgaaaaaag
      661 cagcagcaaa gaaagctact aagaagccgg cagtaaaaaa agctgcagct aagaaaaaag
      721 tagctaagaa aactagcaaa aaagcataac ttttaattta gatttacacg gagcatagtc
      781 atggcaagca agaaggccgg cggtagtaca aagaatggtc gcgattcaca aagtaaacga
      841 cttggcgtaa agagatttgg tggtcaagag gtccttcctg gaacaatcat cattcgtcag
      901 cgtggtacta aattccatgt tggtaataac gtaaaaatcg gacgtgacca tactatctat
      961 tcagtaatcg aaggattggt taaatttgag cgttactcta aagagcgttt caaaattagc
     1021 gtatatccta agaccgcgta atagatataa gacaccaatt agttttgtac aaacgaagct
     1081 aattccagta cttagagagg ggccgatgat cggtcccttt ttgcatttta gccctgttat
     1141 ttagggtatg gtattttatg aagtttattg atgaagttac agtcacagtt tcctcaggcc
     1201 atggcggagc aggggcggtt agtttccgcc gtgaatccat ggctccccgt ggcggaccag
     1261 atggtggtga tggaggaaag ggaggcgatg ttatcttgcg agcctccaaa catcttaatt
     1321 cgctagttga tattagaaat aataaaaaat atcccgcgcc tgatggccaa cctgggtctg
     1381 gctcacaatg ttctggccat aatggcgagg attttgttct cgtaattcct gaaggaactc
     1441 ttgtaaaaaa tatggacggt gaggttcttg tcgacatgac agggatcgaa caatacacgc
     1501 tgcttaaagg tggacgtggc ggtaaaggaa ataccttctt taaaacagcc accaatcagg
     1561 ccccagagta tgctcagcca ggtgaagaag gccaacaact cgagattcgc ctagagctta
     1621 aattgattgc ggacgtaggt attataggat tccctaatgc tggaaaatct actttgattt
     1681 ctagaatttc ggcagcgaaa cctaagattg cagattatcc ttttacaact cttacgccca
     1741 atttaggtgt agttaaagcg gcagacttta aatcttttgt ggttgccgat attccgggac
     1801 tcgtaaaagg cgctcaccaa ggcgtggggc tgggaattca atttttaaaa catattgagc
     1861 gtacaaggtt ttttattcat ttaatagatg cctccgggat gtctggtcgt gatcctatta
     1921 atgattatga agatattaat aatgagctca aaatgtacga ccaaaacaat caagagaaag
     1981 agggcttttt ttctctttct gaccgtgagc agtttgttgt tcttaataag atagatactt
     2041 taagtgaaga tcaacttttg aaactgaaaa gtagttttaa aaagaaaacc ggcgttgaac
     2101 ctctagcaat atctgcagtg acggggaaaa acattaaaga gttgatcacg gatttaacgc
     2161 gtaagatttt tcaaaaggat gcagtatgag aatcggtatt ttcggtggaa gtttcaatcc
     2221 cccccatatg ggtcacctca acagtctcgt gactgttcag aaaaaagcgg gcctcgataa
     2281 aattcacgtg gttccagcag cacaaaatcc tcttaaaact caagttgctg gtccgacaaa
     2341 tgaacaaaga cttgagcttg ttaaattggc gcttaattcc tacggaaacc aattctttgt
     2401 cgatgaccag gaaatccgtc gtggaggctt aagttacaca atagacacgg ttatgaacct
     2461 tcgtagtaag gtgggttctg aagatttatt tttgattatt ggcgcagacc aattcgaaga
     2521 attcagtcag tggaaagatt atcagaaaat tttaactgag gcgaatttga tcgtca
//
LOCUS       DATBTE010000042         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_4739, whole genome shotgun sequence.
ACCESSION   DATBTE010000042 DATBTE010000000
VERSION     DATBTE010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4739"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..973)
                     /gene="pstB"
                     /locus_tag="VIG33_01665"
     CDS             complement(188..973)
                     /gene="pstB"
                     /locus_tag="VIG33_01665"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY8269569.1"
                     /translation="MNSTKTVNLAVRAEVKNLDFFYGPKQVLKKVSMPIFENRMTALI
                     GPSGCGKTTLLRCFNRMHDLYATTNYHGEILLQPGDRNILGAKIDPMEVRMHIGMVFQ
                     KPNPFPKSIFENVAYGLRVRGVRKKSFIEERVVKSLQMAGLWSEVKDRLDSHATDMSG
                     GQQQRLCIARALATEPEILLLDEPTSALDPISTAHIEELITELKKEITILMVTHNLHQ
                     AARTADYTAYMYLGEVIEFGTTDQIFTTPNDTRTENYITGRFG"
     gene            complement(981..1934)
                     /gene="pstA"
                     /locus_tag="VIG33_01670"
     CDS             complement(981..1934)
                     /gene="pstA"
                     /locus_tag="VIG33_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY8269570.1"
                     /translation="MSLDLNLNSGHGSKSIDPVVRNIRQRKLIDFLFAMAGLLSLLFA
                     LVTLLALVIDLAVTGVPRLSTEFFLNFPSRFPEKAGILSAWVGSICVMLTTAFCAIPL
                     GVAAGVYLEEYAPKNWMTQLIELNIINLAGIPSITFGLMALGIFVYKLGFGQSILTAG
                     LTLGLLVLPIIIVTTRESVRAIPSTIREASYAVGASKWQTIRYHILPYSFGGILTGMI
                     ISLSRAIGETAPLITIGALTFVAFLPTAPVHPDFPFLSFQWLKDPFTVLPIQMFNWLS
                     RPQSEFHVNAAATGIVLLLMTLVLNGVAIYTRYRFRKKMKW"
     gene            complement(1931..>2844)
                     /gene="pstC"
                     /locus_tag="VIG33_01675"
     CDS             complement(1931..>2844)
                     /gene="pstC"
                     /locus_tag="VIG33_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEY8269571.1"
                     /translation="AEFTAANHPVRRLRRFKEKTIEFFLFLAAASSVLVTAGIVGILV
                     VESLPFFAHVSLKDFLTDTEWTPLFENAHYGILPLLCGTFLSTLIALVVAIPLGTISA
                     AYLSEYVAHGPREILKPILELLAAIPTVVYGYFALLFVTPLLQKVFPEMGGFNILSAG
                     IVIGIMIVPYVSSLSEDAMRTVSSHLREASFAMGASRFQTTFRVVIPSAFSGMTSAYI
                     LGISRALGETMVVAIAAGMQPNLTLNPTQPAATITAFIVQVSLGDLPHGSVGYQSIYV
                     AGLALLVLTLSFNIIGMWLRLKFQERE"
BASE COUNT          872 a          713 c          621 g          638 t
ORIGIN      
        1 gtaagtagcc ctggatgatt ctctggattc atccctaaga aatgaacctc ccaagatata
       61 gcggctctct tttaaaagaa gttctggcca cgatggccag aactcagcgt tcccatggat
      121 ggaggcacct cgcgcaaccc ctcgactgct gggcaaagac gcgaacattt ggctccttac
      181 gaaaaggtta gccaaagcgt ccggtgatat aattttccgt acgggtgtca ttgggggtgg
      241 taaatatctg atccgtggtc ccgaattcga tcacttcgcc taaatacata taagccgtgt
      301 aatccgctgt gcgggcggcc tggtgcaaat tatgggtcac catcagaatg gtgatttctt
      361 ttttcagctc agtgatcaac tcctcgatat gagctgtgga gataggatcc aaagcactgg
      421 tcggttcatc gagtagcaaa atttctggtt ccgtggccaa ggcccgggcg atgcaaaggc
      481 gctgctgctg cccccctgac atatcggtgg catgggagtc caaacgatcc ttcacttcac
      541 tccacaatcc agccatttgc agcgatttaa cgacgcgttc ttcaatgaaa ctctttttac
      601 gaactccgcg aacacgaagg ccataggcca cgttttcaaa aatacttttt ggaaagggat
      661 tcggtttttg aaaaaccatt ccaatatgca tgcggacttc catcggatca attttggcgc
      721 ccaagatatt tctatcgcct ggctgaagca aaatctctcc atgataattc gttgtcgcgt
      781 acaagtcatg catgcgatta aaacatctta agagagtagt tttaccacag cccgaaggcc
      841 caattagagc cgtcatacga ttttcaaaga tcggcatcga cactttcttc aagacctgtt
      901 taggaccgta aaaaaaatca agatttttaa cttcggcacg tacggccaaa tttactgtct
      961 ttgtactatt cataatccca ctaccacttc atcttcttac gaaatctata gcgagtataa
     1021 atcgctactc cgttcaatac caaggtcatc aacaaaagta caattcccgt cgctgcggca
     1081 ttcacatgga actcggactg aggacgagac agccagttaa acatttgaat cggcaagaca
     1141 gtgaagggat ccttcaacca ttgaaaactc aaaaaaggaa aatcaggatg aaccggcgcc
     1201 gtgggcaaaa aagccacaaa agttaaagcc ccgatggtaa tcaaaggagc tgtttctccg
     1261 atcgcccgag acaaagaaat aatcatccct gtcaaaattc ccccaaatga atacggcaaa
     1321 atgtgataac gaatggtttg ccacttactc gcaccgacag cgtagctggc ctcacggatg
     1381 gtgctcggga tcgcccgcac ggattctcgg gtagtgacaa taataattgg caaaaccaaa
     1441 agccccaggg tcaagcccgc cgtaagaata ctttggccaa atcccagttt atacacaaat
     1501 atacccaagg ccattaaacc aaaagtaatg gaggggatcc cagctaaatt aataatattt
     1561 agctcaatca attgcgtcat ccagttctta ggagcgtact cctcaagata aacccctgcc
     1621 gccactccca ggggaatggc acaaaaggcc gtggtcaaca tcacgcaaat gctccccacc
     1681 caggcggata aaatccccgc tttttctgga aaacgcgaag gaaaattcag gaagaattca
     1741 gtgcttaatc gagggactcc ggtgacagcc aagtcaatga ctaaggctaa aagcgtcacc
     1801 aaagcaaaca acaatgaaag gagccccgcc atggcaaata aaaaatcgat cagcttacgc
     1861 tgccggatgt tccgcaccac aggatctatt gatttacttc catgaccaga gtttaggttt
     1921 aagtctaagc tcattctctc tcctggaact tcagacgcag ccacatcccg atgatgttaa
     1981 agcttaaggt caacaccaac aaagccaagc ctgccacata aatagactgg taccccacag
     2041 aaccatgagg caaatcccct aggctcacct gcacgatgaa ggccgtgatt gtagccgcag
     2101 gttgcgttgg gtttaaagtc aaattcggct gcattccagc agcgatggcg acgaccatgg
     2161 tttctcccag agcccgtgaa atacctaata tatatgcgga agtcattcca gaaaacgcgg
     2221 agggaatcac cacacgaaag gttgtttgaa agcgcgaagc tcccatggca aaagaggcct
     2281 cacgcaaatg actggacacg gtcctcatcg catcttcact gagggaactt acataaggca
     2341 caatcataat tccaatgacg attccggcgc ttaaaatatt aaacccaccc atctcaggaa
     2401 aaactttttg tagcagcggt gtcacaaaca aaagtgcgaa atagccatag accacggtgg
     2461 gaattgccgc caacagttct aaaataggct ttaagatttc ccgtgggcca tgagccacat
     2521 attcgctcaa ataagccgca gaaatagttc ccaagggaat ggccacgacc aaggcaatca
     2581 gggtcgacaa gaaagtgccg caaagcaaag gcaaaattcc ataatgggca ttttcaaaaa
     2641 gaggggtcca ctctgtatca gttaaaaaat ctttaagact gacatgggca aaaaatggca
     2701 aagattcaac caccagaata ccgacaatcc cggctgttac caggacgctg gaagcggcgg
     2761 ctaaaaataa aaagaattcg atcgtttttt ctttaaaacg acgaagccgc cgaaccggat
     2821 ggttggcggc agtaaattcc gcaa
//
LOCUS       DATBTE010000043         2613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2190720, whole genome shotgun sequence.
ACCESSION   DATBTE010000043 DATBTE010000000
VERSION     DATBTE010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2190720"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VIG33_01680"
     CDS             <1..466
                     /locus_tag="VIG33_01680"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEY8269572.1"
                     /translation="AIFPEWISSRSLLMSIMAALVIAVMSDFWGQDSDRLPALFLTQS
                     VLYFIMGFCLWGFSNLHPLSILLNLIFAPLIGAVIFPLSLLVVLIPPLAIVFDLMMNT
                     FTWLLQKSSAVLGNPGEVAPLSPLPQWILFFGLLISSYFYLVAKKRRKAQDA"
     gene            459..1241
                     /locus_tag="VIG33_01685"
     CDS             459..1241
                     /locus_tag="VIG33_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEY8269573.1"
                     /translation="MLKHSFSTAFVAFLLVSLSAIPLKNQGAAKSFWIIWNVGQGQWS
                     TLAEKNSCDHFDMGGERNPLRQVKKICADKMNRIYLSHWDWDHISFAGKAKSLSKDIC
                     LRVPPLGTSSLHKMKLLKVYRPCSSTQQEDFPLKELTHFTAMDSMKTKGKNSNELSHV
                     LLVAKNFLIPGDSTTQQEKTWSTDTPMQKVKFLLLGHHGSRTSTSEGLLARLPQLKMA
                     VASARFAKYGHPHLEVVQRLKRYHVVLLKTEDWGNLWFEISL"
     gene            1396..2526
                     /locus_tag="VIG33_01690"
     CDS             1396..2526
                     /locus_tag="VIG33_01690"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8269574.1"
                     /translation="MRQLTPFILNDLQKKMAFIGGPRQCGKTTLAKSLLKNQFAGLYL
                     NWDRVSDRKKILAGQWTDEDQLLVFDELHKYRKWKTLIKGFYDTEKEKHKFVVTGSAR
                     LDTYQRGGDSLLGRYHYWRLHPFSLYDIPRGISREETLKRLISVGGFPEVFLDNDERE
                     ARRWREERARRILQEDVRDLEHLQHVDLLELMLDHLQDRVGSSLAVSNIAQEIGVSPV
                     TASKWLNILEKMYLIFIVRGYDKKLTRAVHKPIKVYFYDNAEVRGDESVRFENLIATH
                     LLQKNHFFEDYHGYKMGLFYVRDKNQREVDFLVTQNGKPMELIEVKVSDETPAKNLIS
                     FGELLKVPHATQVILHARKSWSKGQLRIQKPLDYFGNIKAGY"
BASE COUNT          741 a          577 c          557 g          738 t
ORIGIN      
        1 tgccatcttt cctgagtgga tttcctcgcg ttcacttttg atgagcatta tggcagccct
       61 cgtgatcgcc gtgatgtcag atttttgggg tcaagatagc gaccgccttc cagccctttt
      121 tttaactcag agtgttttgt attttattat gggattttgt ttgtggggat tttccaatct
      181 ccacccgctc agcattttat taaatttaat ttttgctccc ctgattggcg ctgtgatttt
      241 tcctctgtct ttattagtgg tcctgattcc acctttggct attgtttttg atttaatgat
      301 gaatactttc acttggcttc tgcaaaaaag ctctgctgtt ctcggcaacc caggagaggt
      361 ggcaccgctc agccctctcc ctcaatggat cttattcttc ggtctcttaa tcagttctta
      421 cttttacctg gtcgcgaaga aacgccggaa agcgcaagat gcttaagcat tctttttcta
      481 cggcttttgt tgctttcctg ttggtttcac tttccgcgat ccccttgaaa aatcaaggag
      541 cagcaaaatc tttttggatc atttggaatg tgggccaagg ccagtggagc actcttgcgg
      601 aaaaaaattc ctgtgatcat ttcgatatgg gcggcgaacg taatcccctc aggcaagtaa
      661 aaaaaatttg cgcggataaa atgaatcgaa tttatctcag ccactgggat tgggatcaca
      721 tcagtttcgc gggaaaggca aaatcacttt ctaaagatat ctgtttaagg gtgccccctc
      781 tcggaacttc ttcgcttcat aaaatgaaac tcctcaaggt ctaccgtcct tgctcttcca
      841 cgcagcaaga ggacttcccc ttgaaagaac tcactcattt taccgcaatg gattcgatga
      901 aaacaaaagg caaaaacagc aatgagttaa gccatgttct tctagtagcg aaaaatttct
      961 taatccctgg agactccacc acccagcaag aaaaaacctg gagcactgat actcccatgc
     1021 agaaagttaa attcctcttg ctgggacatc acggaagccg caccagcacc tcggagggac
     1081 ttcttgcgcg gttaccacag ctcaaaatgg cggtggcctc tgcccgattt gccaagtacg
     1141 ggcatccaca cctggaggtt gtgcagagat taaagcgata tcatgtggtg ctcctcaaga
     1201 cagaggactg gggaaatctg tggtttgaga tttctctctg accagtatca aatcaagaaa
     1261 tgacctatga atctcatcct attcaatata aggcattttt aaacacctat aaaaaattgc
     1321 cttgtctttt ttttacacct gtataaaatt gccttaggtg ctttttctaa tgtaatataa
     1381 ggaatagact gctcaatgcg acaattgacc ccttttattc tcaatgattt gcaaaaaaaa
     1441 atggccttca ttggaggacc tcggcaatgc ggcaaaacga cgctggcaaa atcattgtta
     1501 aaaaatcaat ttgcaggcct ttacctgaac tgggacagag tctcagatcg taaaaaaatc
     1561 cttgcaggcc agtggactga cgaagaccag cttctggtct ttgatgaact tcacaaatac
     1621 cgaaaatgga aaactctcat aaaaggtttt tatgatacgg aaaaagaaaa gcataaattc
     1681 gtggtcacgg gcagcgcaag actggacact tatcaacgcg gcggggattc ccttttaggg
     1741 cgatatcact actggagact tcatcctttt tctctttacg atattccccg tggcatctcc
     1801 agagaagaga ccttaaagcg attaataagt gtcggcgggt ttcctgaagt ttttttggac
     1861 aatgacgaac gagaagctcg gcgttggcgt gaagaacgtg cacgtcgcat ccttcaagaa
     1921 gacgttcgcg accttgagca tctccaacat gttgacctcc ttgaactgat gctggatcat
     1981 ttacaagacc gcgtcggctc cagtttggca gtttcaaata tcgctcaaga gattggggtc
     2041 tcccccgtaa ccgccagcaa gtggcttaat atcctcgaaa aaatgtattt gatttttata
     2101 gttagaggct atgataaaaa actgaccagg gctgtgcata agccaatcaa agtctatttt
     2161 tacgacaatg ccgaagtacg cggcgacgag tccgtgcgct ttgagaattt gatagccaca
     2221 catctgttac agaaaaacca ttttttcgaa gattaccatg gatataaaat gggccttttt
     2281 tacgtcagag ataaaaatca aagagaagtc gattttttag taacccagaa tgggaaacca
     2341 atggaactga tcgaggtcaa agtcagtgac gagaccccgg ctaaaaattt aatctctttt
     2401 ggtgagcttt taaaggtacc acatgcaact caggtcatat tgcacgcgcg caaatcttgg
     2461 tcaaagggac aacttcgaat tcaaaagcct ctggattatt ttgggaacat taaagccggt
     2521 tattagccag tgagaaagac cgagtgcgag atgaattgac gaaattggta gctcacatga
     2581 aacaaaaatg acatttaaac aaaccgaaat aag
//
LOCUS       DATBTE010000044        27906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_482776, whole genome shotgun sequence.
ACCESSION   DATBTE010000044 DATBTE010000000
VERSION     DATBTE010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 27906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27906
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482776"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            354..1820
                     /locus_tag="VIG33_01695"
     CDS             354..1820
                     /locus_tag="VIG33_01695"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8269575.1"
                     /translation="MSDLTKWQLHEWKPKTIKFDWTPREKNLNQASGVCALVDLLVAS
                     PFYDELKKCLPQRHSNNSYHPIHFATLLIAGFWMGYDCLDDLEEFANNPAIKAQFGKI
                     PLAKTFGDYLRDFSGENILALRTLLTRQALYYREALKKDSSITFDMDSTDHEYHGDLI
                     EGLEINYKGKWCLDSLEVFDELGFCYDFDLRSGATFSSVGSVAMLEKILLQHTLRVPL
                     KDDFARLDSAFCTEEMIRLGLNRKLKATITAHGRIGWEREVKNITNWVPWQYSAEEIK
                     EALETGVQLPQVEVGYYMYTPGFAENIRLPVIVKRTFKPYKNLSHKKRAEMIKNNQDP
                     KLGLWEHYAVLSLRGLHPEAPQSILEYHLKRANMENLIREEKINFDLRHFPTKKMYAN
                     QAYALLGLMAHNVFRLVAILDNPEHPHYAKHLRRKFIYLPGEFKNWKTQRLIRVSENS
                     YQEVMKLKTRWVEIFKPPPDPSSEYYSKKSYRRSLTTS"
     gene            complement(2202..2275)
                     /locus_tag="VIG33_01700"
     tRNA            complement(2202..2275)
                     /locus_tag="VIG33_01700"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2241..2243),aa:Cys,seq:gca)
     gene            complement(2329..3777)
                     /locus_tag="VIG33_01705"
     CDS             complement(2329..3777)
                     /locus_tag="VIG33_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8269576.1"
                     /translation="MKIKSLVRENSSLVPVEVEVTLLPGLPTIQFLGLPDQIIKESIH
                     RIKSAIRQQGFDFPRAQQVFVNIRPNHLKKSSRGIELAVATGILWETGQLPSPLVQNH
                     FFIYGELGLSGEVFEPEDLASDFEAGPETQVLTGSLSSVCCPFSRRVIHELKDLPQPR
                     LHAPEMKTLVIERPQEGLEFRFSETHVEILSLAALGGHSLLLAGPSGSGKTTIAKTLG
                     SLMSEPRPEEILEIKKIHKGLAEGESLWRPLINPHHSITPLALIGGGVPPQPGEISRA
                     HRGLLVLDELLEFHPKVQESLREPMEEGALRLSRGTKAIKYPAMAQVVATTNLCPCGD
                     FTPGCRAQCRFSLHKCKSYAQKLSGPLVDRFEILFFTTKRQRSAEETAISGWDLLEKL
                     EKRREWLRKLGRGSVTNARRPISEIENEVDSFFLKHMMNKNFGSERRYQATLRVARTL
                     ADLEMSEYVQGPHIERALHMSWMPFEKLKRWD"
     gene            complement(3880..4764)
                     /locus_tag="VIG33_01710"
     CDS             complement(3880..4764)
                     /locus_tag="VIG33_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY8269577.1"
                     /translation="MELELWIEFFDELQNVRGRSQNTVMAYRRDLELYVEFRKTQSQI
                     SGFYEFMRKHKLSTRSQARVISSLRTYFRFCESRGMKCSELRELRPPKVKVGLPKVLT
                     PHEFQRLFDAAETPDPIKTARNQMTLLFLYGLGVRVTELICLNVSDFNPTDRWIKVLG
                     KGNKERLSPITENLAEHLIYYLQEIRPALLKEATPSVLINDRGNRPSRVDVWRWLAAW
                     SAKAGFAEPVSPHRFRHACATALLESGADLRSIQLLLGHASIQTTQIYTNVTTRSMTK
                     AIDEHHPLAKIKELDLDK"
     gene            complement(4883..5464)
                     /locus_tag="VIG33_01715"
     CDS             complement(4883..5464)
                     /locus_tag="VIG33_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269578.1"
                     /translation="MKTTEILFTALTSLMLLTTACGNTSNGGDPVAAAAPAAATGTDG
                     CINCTGFVAGPPVFSGNVSGGGFRIDNLTVIADQNSLDTAAAQSPGSSPRMSGTYQGM
                     VTGGTFTASGSTCVADGTYQVSGLQVGMISTNLSTQSVSPMWVTLKGPSTIRAPIYIK
                     LADTNNDDIGDSGTVVYLWFYCSGWNSIGMSAQ"
     gene            complement(5512..6039)
                     /locus_tag="VIG33_01720"
     CDS             complement(5512..6039)
                     /locus_tag="VIG33_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269579.1"
                     /translation="MKTQTNTNKMRKLVFVTLASLTVITTACSKNNSGGGNAAPPPVV
                     VTPPVCPIQPCVNGMVGGGQMLYGGTTTSAGYFQAQFQVMGSPSGTGPGSITGVVQIN
                     NYVCQIGQPNLNGPFTIQMTQQGQLNADVFTGTVNLVGPQGMIPAAIEVVPTRTQSTG
                     LFSLWLCGTRNDLNF"
     gene            complement(6063..6836)
                     /locus_tag="VIG33_01725"
     CDS             complement(6063..6836)
                     /locus_tag="VIG33_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269580.1"
                     /translation="MKNSKLILILATLALLGVGCAKEMKASDPAPVTPVAPPVVNPTF
                     PVNAPPGQGAGTAGDNWSYGSSVTFKPDSLEIFNTYVGTHPLNNPTNVKLNVNLVDVG
                     GGRFGGQVKLAYDDAGQHYEGYFAAGTGTNQSFESYGTNKDVGLYHAQYNYWFNGGKN
                     FTGYFQDSYGAIVLVIDNVMTQADAQGAAFLSGSVWFKNFTQTFAPQGTQRYCWFIYN
                     GPYNCRSTTVMDKSNPLPSDGYRRVGTFTGLSKSKAFNQ"
     gene            complement(7279..8055)
                     /locus_tag="VIG33_01730"
     CDS             complement(7279..8055)
                     /locus_tag="VIG33_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEY8269581.1"
                     /translation="MEFLIFLGVLAIVLLTATVKILPEWERGVILRFGRFVGVRGPGL
                     ILLIPGVERILKTDMRTITMDVKPQDIITKDNVSMQVNAVVYFKVVSPEEAITKIEDY
                     YFATSQLAQTTLRSVMGQYYLDEILVHRDQVNQTLQGILDKHTEAWGIKVVMVEVKQI
                     DLPKEMQRAMAREAESERERRAKIISAEGELQRAQKLADASKLISTSPAAMQLAYLQT
                     LTEIAGDKTNTIIFPLPLEIIKPLMGLGQASNPVKEDLKS"
     gene            complement(8092..9396)
                     /locus_tag="VIG33_01735"
     CDS             complement(8092..9396)
                     /locus_tag="VIG33_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEY8269582.1"
                     /translation="MKTLGTFLLGVLILFVGLNSLGAPTPTQTQQNCTVAVTIQDAIG
                     AATLDYLTRAQNFALESHCSSLFVRMNTPGGSLQSTRLIIEQILASPLPYLCLISPSG
                     GHAGSAGAIILMACHVNGGLTATNIGAATPILGTGGETPQDLRNKVVNDTVSWLEGIV
                     ALRGRSSEFAKEIVTEAKAVSAEEAKKLKALDIVANNELEFLQEVQGRKVLVREQKDF
                     AVQVGSLKEFQPDLRYRILNFTADPEIAYLLFMGSLALLYVEITHPGLIAPGVIGGIG
                     LILSLISFHKLGVMWGGLALILLGVAFLIAELFVTSFGILGVGGIISFVMGSVLLFDR
                     NLSGQSLPWTLIGGVTLVLVAIFLGLGTLALRTFRSRRHDTDFDLKTQRVTIVSLDPP
                     GCSGQVEIQGEFWKFICDEELKITDAVEVLDRQGLTLKIKKK"
     gene            complement(9393..9890)
                     /locus_tag="VIG33_01740"
     CDS             complement(9393..9890)
                     /locus_tag="VIG33_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269583.1"
                     /translation="MAAEESITNYFSYVKNVLGVKTIQSARIRQNLANVQTEIQADKC
                     DLLFLNFKQASDQSIFAPECAELLDKMIQAMRLGNRRYLILECDLAGNTKSTTQILQQ
                     YTQKITTPFVMLFSSKPTQNSIQNLGNINFLESYSPSYLLQYPSAKKMAWADLQKVMK
                     EIGVG"
     gene            10115..10321
                     /locus_tag="VIG33_01745"
     CDS             10115..10321
                     /locus_tag="VIG33_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269584.1"
                     /translation="MEIFRFNVVPEQEIKRREKFKYALSHCAVCHGNLEFNYFDTLDE
                     DKVEEVAHCKDCGRKATNQLHSIH"
     gene            complement(10403..10825)
                     /locus_tag="VIG33_01750"
     CDS             complement(10403..10825)
                     /locus_tag="VIG33_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY8269585.1"
                     /translation="MTALDTNILIGIMVSSSSFHEEAMRGLTNINDDLCISPTNVGET
                     LRILTHPKVFESPLKIAKAISVFSDLLESYNIRILEEDINWWRLLPEIEKQIPGLKGS
                     EIFDARIATCLKQNNVKRIFTRDSDFKKYSFLQVTRPL"
     gene            complement(10812..11051)
                     /locus_tag="VIG33_01755"
     CDS             complement(10812..11051)
                     /locus_tag="VIG33_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269586.1"
                     /translation="MKTSLILDDKVFEEAKKEAQKSGRSISEIVSQWAVLGRDLWRQK
                     KGQKVKSFKPLDLGAERIDLTTRKEWMEDLEDDRT"
     gene            complement(11176..11655)
                     /locus_tag="VIG33_01760"
     CDS             complement(11176..11655)
                     /locus_tag="VIG33_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269587.1"
                     /translation="MKMRAWIYIFLTTVFIGVFKAPLAHAENFGLGIILGDPTGLSAK
                     AKLDERHAIDGALAYSSGRHSGLQLHMDYLWDRARSWGTTQGPLNMYYGLGGRLITYN
                     DREDNSQISLGPRGSLGLDFNINNPNLEFFGELAMILELIPSIAADLDAGIGVRIRF"
     gene            complement(11687..13255)
                     /gene="murJ"
                     /locus_tag="VIG33_01765"
     CDS             complement(11687..13255)
                     /gene="murJ"
                     /locus_tag="VIG33_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY8269588.1"
                     /translation="MKKHAFLVGLGIFFSRIAGLVRERVFAHYFGNSDAGDAFKAALK
                     IPNFLQNLFGEGVLSASFIPVYAQLLAKKHEEEAAKVASVVGSLLFVATSVLVLIGVF
                     ATPFLIDVIAPGFHGEKRQLTIEIVQILFPGTGFLVMSAWCLGILNSHRKFFLSYVAP
                     VIWNLAIIGAILGGRHQAPYPLAITASYGLVLGSFLQFLIQFPFALKLGRKIYPSLNL
                     KVESVRTVLKNFFPVVFSRGVVQVSAYIDNVLASLLPTGAVSALAYAQTLYLLPISLF
                     GMSISVVELPAMSQATGTDQEVSLYLQTRLNGGLQQIAFFIIPSVVGFLALGDLIVGA
                     IFQTGAFDHDSTNYVWAVLAGSTVGLLASTLGRLYSSTFYSLRDTRTPLKFALVRVFF
                     TTILGVFCAFYLPKLLGVDLRWGTTGLTASAGLAGWIEFYLLRHTLNKKIGDTGLKFS
                     FQLKLWGVAIVSAALAFGIKFLLNNFAHVILRAMIVLGVYGLLYFGIGYLYKVEQAVS
                     LIDKVKNKLKIKLK"
     gene            complement(13252..15129)
                     /gene="uvrC"
                     /locus_tag="VIG33_01770"
     CDS             complement(13252..15129)
                     /gene="uvrC"
                     /locus_tag="VIG33_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY8269589.1"
                     /translation="MSQKFDELRELIKGYPIQSGVYLMKSAQDKIIYVGKAKNLRNRV
                     RSYFTDSKDHTPKTRLLVQNITHIEYILTKTEVEAFLLEASLIKKHRPKYNIRLRDDK
                     AYPYIRFTWNEDYPRLYLSRKVKKDGSLYFGPYTSGGAVFGTIRFLNRTFKIRDCTDA
                     MLKARKRPCMTHQIGRCTAPCVEYISSEEYRSEVEGALLFLKGKNKTVLKTLKQKMLL
                     AAEEEKFEVAARLRDSIESVKSILEKQSVINDTSEKDQDAIGYFGDDRGTLIETVHVR
                     QGRVIGTRSHFISVLDVVDPASDAREWLVSFMNQYYEDNIIPDEVLLPTEIGGDLNKL
                     MEAVLLERSGHKSVVRFATDEKGRSLVEMAHQNAKAHFEKYVSKSEEKMRGLDEIKER
                     FGLSQRPTRIECYDISNFQGTETVASQVVFEEGVPSKENYRRYKIKTVEGSNDFASMY
                     EVLSRRFKHTEYEDPHLLVVDGGKGQLSQAMRILEEIGRKEIPVVGLAKARTESDFQS
                     SEVSSTEERFFLPGRQNALTFRSNSEALHILVGIRDEAHRFAITYHRKLRENTSLESE
                     LDFVIGLGEKRKKVLLTHFKGVQELKEADPEEIAKLKGFNRVLAERVLLQLNESEDED
                     S"
     gene            complement(15133..17208)
                     /gene="uvrB"
                     /locus_tag="VIG33_01775"
     CDS             complement(15133..17208)
                     /gene="uvrB"
                     /locus_tag="VIG33_01775"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEY8269590.1"
                     /translation="MAVKKSFELVSEFKPSGDQPKAIEDLMQNFKLGLNHQTLLGVTG
                     SGKTFTMAHTIAQLNEPALVLAPNKTLAAQLYAEFKELFPKNAVEYFVSYYDYYQPEA
                     YIPSTDTYIEKDSAINEQIDRMRHSATRSLFDRRDVIIVSSVSCIYGLGSPEAYEGMM
                     IHLVANSELKRDHFLHELIRIQYTRNNVDFHRGTVRVRGDVVEVFPSYEEERAIRVEF
                     FGDYVERISWVDPLTGQVLEELDQIGIYPGSHHVTGDENLNRAIKTIQEELREQLVAL
                     KNNVKLLEAQRLEQRTYYDIEMIEQMGFCQGIENYSRHMTGRGPGEPPPTLLEYFPKH
                     FVTFIDESHVTVPQIGGMYRGDRARKTTLVEHGFRLPSALDNRPLNFQEFERMMDKVV
                     YVSATPGNYEFEKSEGVIIEQIIRPTGLIDPVVEVRPVRHQVDDLLAEIRERVKNGDR
                     VLITTLTKRSAEDLTEYYESLGVKVKYLHSEIQTVERTEILRDLRLGVFDVLVGINLL
                     REGLDIPEVSLVGITDADKEGFLRSQRSLIQTIGRAARNLNGKVILYGDRITDSMRKA
                     IDETDRRRRIQTEHNLKYGITPASVKKRIKEGLGDVFDGSVGAHLVDGMERKQAGMAQ
                     KFGNEPNKIQEEIEKLRKKMKKHSSDLEFEEAAKIRDEIKRLQILDLSLRSGEVEKET
                     ETIIEGLKK"
     gene            17987..18322
                     /locus_tag="VIG33_01780"
     CDS             17987..18322
                     /locus_tag="VIG33_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269591.1"
                     /translation="MKSLFFILIAALSAFANAGELDGMTFCRKVETGGMFGQPKGTRE
                     HCVSFKEDVMTDNANTFFGNPPECFSYTIENSKIINSDKNSDTGYEIQGEAIVVPETG
                     AVLTLKLNR"
     gene            complement(18439..19887)
                     /gene="cysS"
                     /locus_tag="VIG33_01785"
     CDS             complement(18439..19887)
                     /gene="cysS"
                     /locus_tag="VIG33_01785"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY8269592.1"
                     /translation="MLRIHNTESRKLEDFEPLGKGKVKIYVCGPTVYSFLHVGNFRGV
                     VFFNLVRNWLENLGYQVEYALNFTDVDDKIIESANKQGVKPEDLAEKYIAEYKKDFSL
                     LGLRPHDHNPKVTESMDEIRSIVAQLIENKKAYVADGDVLYSIESFSSYGKLSGRQVE
                     DLKAGARVEIDEKKKSPLDFALWKGAKPGEPSWPSPWGPGRPGWHIECSAMIEKIFGE
                     QIDIHGGGTDLIFPHHENEIAQSEGCSGKHFVKYWMHWNMLNFGNQKMSKSLGNFVTM
                     REFLEKYNSEIYKWMILSVHYRSPSDFSDEAVDRAVSNLARIYSATAVAESLLGEATV
                     PVDVGFEKITAEAWTKVSEALNDDFGTPNAMAALFEVVRQFNTQVKRGIKPNPAVLGK
                     AQSFLNFVRKLGALMAMFQEPANGFLLKLDNMLLEKMNLQRAAVDQLVAERSQARADK
                     DFAKSDEYRKKLTELGISVSDTPEGSFWEVTK"
     gene            complement(19891..21360)
                     /gene="gltX"
                     /locus_tag="VIG33_01790"
     CDS             complement(19891..21360)
                     /gene="gltX"
                     /locus_tag="VIG33_01790"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEY8269593.1"
                     /translation="MSKVRVRFAPSPTGYLHVGGARTALYNYLFAKKQRGEFILRIED
                     TDEARSTQESLRGVVDDMVWLNLLWAEGVDPVTLQDQGPLGPYKQSERLPIYKAIANE
                     LLNSGKAYYCFLTDEEITVQREAAIQAGRPPQVESPYQDWPLAKAQEKLAAGEKAVVR
                     FKTRELKKDYIFTDLVRGEVKFPSDMVGDFVILRSGGMPVYNFCCVIDDHLMKMSHVF
                     RAEEHLPNTLRQLMIYEAMGWQAPQFAHISLILDEDRQKLSKRKGAVACGQLKEEGYL
                     PSAVLNFIALLGWSHPEGKEIMTVDEMIQAFDVTRLNPSGAVFDRVKFKWMNAMHLRA
                     LPNMELWKEIQPFLAREKMQLPQDPMWQEKSIVAFKTSMETLADAIELYKPLNDNSFV
                     IQPEADEALAWEPTKAVLTTWKELVQASGKDYLSEEEFLKLQDEVKNKTGAKGKNLFM
                     PIRVAVIGKPHGTELKILVPLIKTASLVDRVNKVLAKIG"
     gene            complement(21447..21941)
                     /locus_tag="VIG33_01795"
     CDS             complement(21447..21941)
                     /locus_tag="VIG33_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEY8269594.1"
                     /translation="MVTFNVGDNAVYPGHGVGKVTAIETKEILGNKQTFYSIQILDTG
                     MKIMIPRDNVESVGLRPIISKEEAGRVVSILRETDVKIDTQTWNRRYREYMEKIKTGS
                     VFEIAEVLRDLFLLKVDKELSFGERKMLDTARTLLLKELTLATSQEELFKEEEVKAIF
                     GVVP"
     gene            complement(22100..24229)
                     /locus_tag="VIG33_01800"
     CDS             complement(22100..24229)
                     /locus_tag="VIG33_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8269595.1"
                     /translation="MNLLSKNILVIASIAASISSLAHAGTVLRLNVGRVESKQVESLM
                     SAQAGNLSGPQDLIIQFKNLITEKDKAELKAMGAEIFGYLPDDALMVRAPIAKIQQWR
                     THKAIEAVLPFKADYKVSTSFGAASIFNKEEQTSILVKTFKANETRDIAVKIKSLAQS
                     TVHYADGKSIVAVVPRGMVRAVAAFQGVEHIQPYVEMKSMHMVFDEGTPTPIVQGTGD
                     YTDLNDYETGTKVANFDPAWAMGYKGRNQVVAMADTGLDMGENQLTGDFKDAVLKGYI
                     FGLFSKAWDDPMGHGTHVAGSILGRGTASGGKIRGGAYEASLIPEAMWSPMMKNLTVP
                     SKIQDLFSKAYADGARVHSNSWGSARNFGAYDNYASSLDEFTFNNPDMLIIFAAGNSG
                     IDANKDGRIDPNSVSSPGTSKNALTVGASENLISTGGIQSPINKLRSAADHWSAEPIA
                     SSYISDNINGLAMFSSRGPTVDGRTKPEIVAPGTNILSNRSHVVGASPLWGAYNVDYA
                     YSGGTSMATPLVAGGAAVVRQVLQEKFQLASPSAALLKATLLHTAIDMFPGQYGEVGV
                     ARGQELATRRPNSDEGYGRMDVGSAANLSSNTHFVDDKSGLAQGEEHSFTVILNGSGS
                     LLVNMVYTDAPASPDAAVALVNDLDLTLTGNGQNVAPSDHVNNNEVIELSNLPAGSYT
                     LTVKAPKVPQGQNGRQPYALVYTAREN"
     gene            complement(24311..26449)
                     /gene="recG"
                     /locus_tag="VIG33_01805"
     CDS             complement(24311..26449)
                     /gene="recG"
                     /locus_tag="VIG33_01805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY8269596.1"
                     /translation="MDSLTRKSKDENLINMALRLDTSLQYLKGVGPKLASLFARHGIR
                     TVGELLEFYPRAYEDQRAARNIASLKVGDIVSVKAQVVSVHSINMGNSHRKMYDVVVR
                     DSSGQIRCKFFRIPYKGYFERFTPFKEVRVVGKVSDYRGKIQFQHPDIRDIDPEEENH
                     DSLIPLYTEIEGLGTAKIHRFIKAALSLLPEQEWPPEQLPSWIVKKYNLISRRKALKL
                     LHDPETALGVQYAELKTPAHRRTIFEEFFWLELYLASKKIGFEKEKGLAFSSDGKKLE
                     ELQKSLPFSLTNAQKKAFQEIKTDLEKSSPMHRMVQGDVGSGKTLVCFMAAVAASESG
                     LQSCLMVPTEILAEQHFKNAQQLLAPLGLRLGLLTGKTKTAERKQLLQQLAVGEIDFL
                     MGTHALLEEEVQFKSLALVIIDEQHRFGVEQRGALKGKGLSPHFLVMTATPIPRTLAM
                     TVYGDLDVSIIDEMPPGRSPIQTRAIFDSKKNAALQFMQEQINKGRQAYIVYPLVEES
                     EKIDLKNAVSEFQNLQRQFPNIKMGLLHGKMKPQEKEEVMTHFRKGDLQVLVSTTVIE
                     VGVDVPNANMMIIEHAERFGLSQLHQLRGRVGRGSHKSYCILIMGYAVSEEAKLRTEF
                     MEKTTDGFKIAEFDLEMRGPGEFMGTRQSGLPGFKMANLVRDMSILQEAREAAFEVLR
                     KDNQLKLAENQSLREELTKTHGPANLAGIA"
     gene            26464..27411
                     /locus_tag="VIG33_01810"
     CDS             26464..27411
                     /locus_tag="VIG33_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY8269597.1"
                     /translation="MQQEAYFRIRLSTIRPSKATSFDIYIQINGQMVLYLKTGDALSS
                     LKIESLHNKDTGNTFFVLTSQKQAYRDWVKEEMNSAELNPFEKSKILRESSVALMEDL
                     FENPDVNTALDESRPIIKNFIDFMEQAPEAMGYMISLSGHDFYTYNHSLDVSIYSLGL
                     GSILSFNKTDLEDLGVSSLFHDIGKRNVSLDILCKKGGLDDAEWEQMKRHPQYGLMIL
                     NQNPNISDAIKAACFEHHESFTGNGYPQGISGQEIHPFARIVAITDTYDAMTTQRSYN
                     APLKPFDAVIMMKEKLAGRYDPEFLKAMYSVLFKLKGAA"
     gene            27424..27897
                     /locus_tag="VIG33_01815"
     CDS             27424..27897
                     /locus_tag="VIG33_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269598.1"
                     /translation="MLKLLLNTYLAFLLIPLLTLAIEPTNPTGLCDRFLGDQEKTACM
                     VKTSQTELDWYASSSCALQQDDKNFMNCLEEIKGASFSPEALELCAKSVDLTDEARLS
                     CIKKIRNKDYTRSQIKKCSEAGVPVAVENCLSNSSRTPATAGVTKSGFQTLEIKK"
BASE COUNT         7730 a         6963 c         5788 g         7425 t
ORIGIN      
        1 aaatgccgaa agaggatttg cttcatcggc aagacgtcat gaatgctttc agtgaatctc
       61 tccgagataa tcattgggtt gaaattaaat aggtattcct gacaaatcaa actgtcactg
      121 cttgactcct ttttatttgc agcgagactt catctcttca tagacccaat ttgccaaaac
      181 atagacccag atccgatttt aaagaatcaa taaactcacc aataatttag taatttttgt
      241 catttgacca ttatttcttg ggcgttaacg cttttcctcc gtgggtgcag ggcaataaaa
      301 ctaaagatcc caaccggcaa aaaagttaaa ttttgcagac aaggatggat cttatgtcag
      361 acctcacaaa gtggcaactc catgagtgga agccaaaaac aataaagttc gattggaccc
      421 caagagaaaa aaatctaaat caagcttcgg gcgtgtgcgc gcttgtcgat ctccttgtgg
      481 cttcgccctt ttatgatgaa ctaaaaaaat gtttgcctca aaggcacagt aataattctt
      541 atcatccgat tcactttgca actcttctga tcgcaggatt ttggatgggc tatgattgct
      601 tggatgacct tgaggagttt gcaaataatc ccgccatcaa agcgcagttt ggaaaaatcc
      661 ccctggcaaa aacatttggt gattatctca gggacttttc aggggaaaat attttagcct
      721 taagaaccct gctgactcgc caggctcttt attaccgcga ggctttaaaa aaagattctt
      781 ccattacttt tgatatggac tcaactgatc acgagtacca tggggatctg attgaaggac
      841 ttgagatcaa ctacaaggga aagtggtgtt tagactcact tgaggtcttt gatgagcttg
      901 gattttgtta tgactttgat cttcgcagtg gcgccacatt ttcctctgta ggttcagttg
      961 caatgctaga gaagattctc cttcaacaca ccctgcgtgt gcccttaaaa gatgatttcg
     1021 ccagacttga ttccgcattt tgcaccgagg aaatgattcg gctaggttta aatagaaaat
     1081 taaaagccac catcacagcc catgggcgca tcgggtggga gagggaagta aaaaatatca
     1141 ccaactgggt tccgtggcag tatagcgctg aagaaattaa agaagccctc gagactggag
     1201 tacagcttcc acaggttgaa gtgggctatt acatgtatac cccgggcttt gctgaaaata
     1261 ttcgcttgcc agtgattgtg aaacgcacct ttaaacccta caaaaatctc tcccataaaa
     1321 aaagagccga gatgataaaa aataatcaag atccaaaact tggactttgg gaacactacg
     1381 ctgttctttc actgcgagga ctgcaccctg aggcgccgca gagcatttta gaatatcatc
     1441 taaaacgtgc caatatggag aaccttattc gcgaagaaaa aatcaacttt gatctcaggc
     1501 actttcccac taagaaaatg tacgccaatc aagcctacgc cctcttgggt ttaatggctc
     1561 ataatgtttt tcgtttagtg gcgatcttag ataaccccga gcatccccat tacgccaaac
     1621 acttaagaag aaagtttatc tatttaccag gggaatttaa aaattggaaa actcagagac
     1681 ttattcgggt ctccgagaac tcttaccagg aggtgatgaa attaaaaaca agatgggtgg
     1741 aaatctttaa gccaccccca gatccttctt cagagtatta ctcgaagaag tcttatcggc
     1801 gttctttgac aacctcataa ttttttttgg aatcagtttc ttggattctt agaagatttg
     1861 agggggattg aaggacttga actcacgcac accacttaca ccaccgcaac aaggccgtgg
     1921 tcggcctaaa atcacccgca ggggcctgac ggtgagcacc cccttcgtaa gtccactcat
     1981 ctcacgaggt tttttgccaa tttctaggca tggcagtgcc tccgaccgtt tttcacggca
     2041 tcggaaaaat attttctatt tgattttttt tcttggagag ctttgacgcg ccttaaattc
     2101 ggatttgggt ttgagctttt gaagccccgt aagtgattga aatcttttgt taatgttttt
     2161 tgctatttag ctttggtttc gagacttttt gcggggagat ttggaggtgg tgaccggact
     2221 tgaaccgatg tacgagcttt tgcagagctc tgcctaaccc ctcggccaca ccaccctatt
     2281 gggttaaaaa gaaagatcaa attaatagtt tatgagttct gagtcaactt aatcccaacg
     2341 cttcagtttt tcaaaaggca tccaactcat gtgaagagcc cgctcgatat ggggaccctg
     2401 tacatattct gacatttcaa gatctgccaa agttctggcg actcgaagcg ttgcttgata
     2461 gcggcgctca gatccgaagt ttttgttcat catgtgctta agaaagaagc tatctacctc
     2521 gttttcaatc tcgctgatcg gtcgacgcgc attcgtcacg ctccctctcc ccaacttccg
     2581 caaccactcc cgcctctttt caagcttctc taggaggtcc caccctgaaa tagccgtctc
     2641 ctctgcagat cgctgtcgct tagtggtgaa aaaaaggatt tcgaaacgat ccaccaaggg
     2701 acctgacaac ttttgcgcat aagacttaca cttgtgcaaa gagaaccggc actgagcccg
     2761 gcagccaggc gtaaaatcac cgcaggggca aagattcgtc gtggccacca cctgcgccat
     2821 tgcgggatat ttaatcgcct ttgtgccccg agaaagcctt aaagcacctt cctccatggg
     2881 ctctcgtaag ctctcctgaa ctttgggatg aaactccaag agttcatcta aaaccaaaag
     2941 cccccgatgg gctcggctga tttctccggg ctgtggagga actcctcccc caatcaaggc
     3001 taggggtgtg atggaatggt gaggatttat aaggggcctc cataaacttt ctccctctgc
     3061 caagccctta tggatctttt taatttctaa aatctcttcg ggccttggct cactcataag
     3121 gcttcccaag gttttggcaa tggtcgtctt ccccgaccct gaaggcccgg ctagcaggag
     3181 ggaatgcccc cctaaagccg ccaaggaaag gatttcgaca tgggtttcgc taaaacgaaa
     3241 ttccaaaccc tcttgaggtc tttcaatgac taaggttttc atctcgggag catgaagtcg
     3301 cggctgtgga aggtctttga gctcatggat cacgcgacga gaaaaaggac aacagaccga
     3361 acttaaactg ccggtcagca cctgcgtctc agggcccgcc tcgaaatcgc tggccagatc
     3421 ttcaggttca aaaacttcgc cacttaaacc cagttctccg taaataaaaa aatgattctg
     3481 caccaagggt gacggcagtt gccctgtttc ccagaggatt cctgtggcca ccgccagttc
     3541 gattccccgc gaagatttct ttaagtgatt cggtcgaata ttcacaaaga cctgttgggc
     3601 ccggggaaaa tcaaagccct gctgacgaat cgcacttttg atacgatgaa tgctttcttt
     3661 aatgatttga tcgggaagcc ccaaaaactg aatcgtcgga agccccggca acagcgtgac
     3721 ttcaacttca acaggaacca aggaactatt ttcacgaaca agagatttga ttttcatgct
     3781 tggctgctta agcaaagccg tcgccaagct gaaagggcct gcaggcagtg tccccgtccc
     3841 aagcttcttt aggttcggac agggctttcg aatttcggac tatttatcca aatccaactc
     3901 tttgatcttt gccaaaggat gatgttcatc aatcgccttc gtcatcgagc gagtggtgac
     3961 attggtatat atttgtgtcg tctggatact cgcatgaccc aaaagtaatt ggatagaacg
     4021 cagatccgcg ccgctttcca gcaaagccgt ggcgcaggca tgacggaaac gatgaggact
     4081 caccggctcc gcaaaacccg cttttgccga ccaagcggcc agccatctcc agacatcgac
     4141 tctcgaggga cgattccctc gatcattaat caaaacagat ggagtggcct cttttaaaag
     4201 cgcaggacga atctcttgca gataataaat caaatgctcc gctaaatttt ccgtgatcgg
     4261 agacaaacgc tctttattgc cttttcctaa aaccttgatc caacgatccg tgggattaaa
     4321 atccgagaca ttcaaacaaa tcagctctgt gactcgcacc cctaggccat aaaggaacaa
     4381 caaagtcatt tgattgcgtg cggttttgat cggatccgga gtttccgcgg catcaaagag
     4441 ccgctgaaat tcatggggag ttaaaacctt cggcaaaccc actttcactt tggggggacg
     4501 aagttcacga agctctgaac atttcatacc gcgggattcg caaaaacgaa aataagtgcg
     4561 aaggctgcta atcacccgcg cctgggaacg ggttgaaagt ttatgttttc tcataaactc
     4621 atagaagccg ctaatttgtg attgggtttt gcgaaactca acatagagtt caagatcgcg
     4681 gcggtaggcc atcaccgtat tctgtgagcg accacggaca ttttgtaact catcaaaaaa
     4741 ttctatccaa agttccaatt ccataaaacc aattacaaaa gattttatgt tacattggca
     4801 agcggctttc gaacttattt ggaacttagg cgctgcgtct cagatattcg ttcgaaagac
     4861 atttctgagt ttcaacaaaa gcttattgtg cagacattcc gatagagttc catccactgc
     4921 aatagaacca tagataaacg acagtcccac tatcgccgat atcgtcatta ttcgtgtcgg
     4981 ccagtttaat gtagatggga gctctgatgg tactgggacc tttcaatgtc acccacattg
     5041 gactgaccga ttgagtgctt aagttggtcg aaatcatacc cacttgcaaa ccactgactt
     5101 gataggtgcc atcggcgaca caagttgaac ctgaggccgt gaaggttcct cccgtgacca
     5161 tcccttgata agttccagac attcgtggac tcgagcccgg ggattgggca gcggcggtgt
     5221 caagactgtt ttgatccgca atcaccgtca aattgtcgat acggaaacca ccgcctgaaa
     5281 cattcccgga gaatacagga gggccagcga caaaaccagt gcagttgata cagccatcag
     5341 ttccagtggc tgcggctggc gccgcagcgg cgactggatc gccaccattg ctggtgttac
     5401 cacaggcagt ggttaataac atcagtgaag ttaaagctgt gaataaaatt tccgtcgttt
     5461 tcatatcatc ctcttttctc aagacgcatc cggcgcttgt tttaggggcc attaaaagtt
     5521 cagatcattc cgagtcccac acaaccacag agagaaaagc cccgtgcttt gtgttctagt
     5581 tggaacgact tcaatagcgg caggaatcat tccctgggga cctactaaat tcactgttcc
     5641 cgtaaataca tccgcattca attgtccttg ttgagtcatt tgaatcgtaa aggggccatt
     5701 taagtttggt tgtccgattt gacaaacata attattgatt tgaacaactc cagtgattga
     5761 gccaggccct gtgcccgaag ggcttcccat cacttggaac tgggcttgaa agtagccagc
     5821 gcttgtggtt gttccaccat aaagcatctg gccgccgccc accatcccat tcacgcaagg
     5881 ttgaatagga caaaccggcg gagtgaccac gacaggaggc ggagccgcgt ttccaccgcc
     5941 actgttgttc ttagagcagg ctgtcgttat cactgttaaa gatgcaagag tgacaaacac
     6001 aagcttcctc attttgttag tattcgtttg agttttcata aacacctctc gtaactttac
     6061 tttcattggt taaatgcttt tgattttgat aatccagtaa acgtccccac tctgcggtat
     6121 ccatcagaag gcaagggatt ggacttatcc atcaccgtgg tggaacgaca attgtaagga
     6181 ccgttataaa taaaccagca atatctctgg gtgccctgag gagcgaatgt ctgtgtgaag
     6241 tttttaaacc agacagatcc actcagaaag gcggcacctt gcgcatccgc ttgagtcatc
     6301 acattatcga tcaccagaac aatggcgccg taggaatcct gaaaataacc cgtaaagttt
     6361 ttaccgccat tgaaccaata attgtattga gcatgataaa gacccacatc tttattggtg
     6421 ccataacttt cgaaactctg atttgttccg gttccagctg cgaaatagcc ttcatagtgt
     6481 tgaccggcat catcataagc cagcttcacc tggccaccaa aacggccacc gccgacatct
     6541 accaaattca catttaattt tacgttcgtc ggattattga gcggatgagt tcccacataa
     6601 gtattaaaaa tctccaagct gtctggctta aacgtgacgc ttgagccata agaccaattg
     6661 tctccagcgg taccagctcc ttgacctgga ggagcattca ccggaaatgt cgggttcaca
     6721 acaggaggag ccaccggcgt caccggagcg gggtcgctgg ctttcatctc tttggcacac
     6781 cctacgccca ataaagccag ggtcgccaat attaagataa gcttcgagtt tttcataatt
     6841 gcctctctgt tatgaggtcc tccaacagtg agaacctctc actcacaagc aatgcgagtc
     6901 ctgtgccgtc catgccacga acccataccg gggataaccc ttcagatttt cagggttttt
     6961 agccatctat tcagaatgag acaaagtccc taaagaccct ctaaaaggga tgagacctat
     7021 ttttgtcaca ggagagttag aaaatcagac acttccaaag ccgactgatg gcttgcgagt
     7081 ggaaagtctg actagacatc ggggaaacag aataaaaaca aactaaattt tattatattt
     7141 attcactccc ctcttcagtt ctcaaaagaa gaaccgaaga ggtattcagg agaaacaata
     7201 tgcaaaacag aactttgaac atgcttgtga ttggcgccgt cgcagttttc gtgtgccgcg
     7261 tttttattaa cttttaattc agcttttcaa gtcctctttt actgggttcg acgcctgacc
     7321 caagcccata agaggtttaa taatttccag aggcaacggg aaaataatgg tattcgtttt
     7381 atcgccagcg atctcagtca gagtctgtaa ataagccaat tgcatcgctg ccggagaagt
     7441 cgaaattaat ttagaggcat cggccaattt ctgagcccgc tgcaactccc cttccgcact
     7501 gatgattttc gcgcgacgtt cccgctccga ctccgcctct cgggccatcg ctctttgcat
     7561 ctcttttggc aaatcaattt gttttacttc caccatcacc actttgattc cccaggcttc
     7621 ggtgtgctta tcaagaatcc cttgcaaggt ctgattgact tggtcacgat ggacaaggat
     7681 ttcatcgaga tagtactgac ccatcaccga acgcagagtg gtttgcgcca actggcttgt
     7741 cgcaaaataa taatcttcga ttttagtgat cgcctcttcc ggagaaacga ctttgaagta
     7801 aaccacggca tttacctgca tactcacgtt atctttggtg ataatatctt gcggttttac
     7861 atccatggta atggtgcgca tatccgtctt caaaattctt tccactccgg ggatcagcaa
     7921 gattaaacca ggtccacgaa ctccaacgaa acggccaaaa cgcagaatca ctccacgctc
     7981 ccactccggc aatattttga cggtcgccgt gaggaggaca atcgccagca ctcctagaaa
     8041 aattaaaaat tccacacctt actccttctg cttaattgat atcttcgtca tttatttctt
     8101 cttaattttc agagtcaaac cctgacgatc taatacttcc accgcatcgg tgattttcag
     8161 ttcttcatcg catataaact tccagaactc accctgaatc tccacctgtc ccgaacagcc
     8221 tgggggatca aggctcacga tagtcactct ttgagtcttt aagtcaaaat ccgtgtcatg
     8281 gcgacggctg cgaaaagtgc gaagcgccag agttcccagg cctaaaaaaa tggccacaag
     8341 cacaagagtc acaccaccga tcaaggtcca aggaagactc tgaccgctga ggtttctgtc
     8401 aaagagcaat acacttccca ttacaaaaga aattataccg ccgactccta agatcccgaa
     8461 gcttgtcacg aaaagttctg cgataagaaa agccacgccg agcagaatca acgccaaacc
     8521 gccccacatc actcccagct tatggaaaga gatcaaggat aaaatcaaac ctatgccacc
     8581 aatcactccg ggagcaatca ggccgggatg cgtgatctcg acatagagca aagctaggct
     8641 tcccataaaa agcaaatagg ctatttcagg atctgcggtg aaattcaaaa tccgatagcg
     8701 cagatccggt tgaaactctt tgagacttcc cacttgcact gcgaaatctt tttgttcgcg
     8761 gacgagaact ttacggccct gcacttcctg taaaaattca agttcattgt tggcaacgat
     8821 atccaaggcc ttaagctttt tagcctcttc ggcacttact gctttggctt cggtcacgat
     8881 ttccttagca aattcagagc tgcgccctcg caacgccaca atcccctcca accaactgac
     8941 cgtatcgttc accactttat tacgcagatc ttgcggcgtc tcgcctcccg tcccgaggat
     9001 cggcgtagca gccccaatat tggtggcagt aaggcctccg ttcacatgac aagccatcaa
     9061 gataatggcc cctgcgcttc cggcatggcc acccgaggga gaaatcaaac agaggtaagg
     9121 aaggggggac gccaggattt gctcaatgat caaacgcgtg ctttgcaaac tgccgccggg
     9181 agtattcatc cgcacaaaca aagagctgca atgactctca agagcaaaat tttgggctcg
     9241 tgtgaggtag tccagggtgg ccgcgccaat ggcgtcttga atggtcactg caacggtgca
     9301 attttgctga gtctgggtcg gagttggtgc tccgagggag ttaagaccaa cgaacaaaat
     9361 aaggactcct aataaaaaag tcccaagagt tttcatccga cacctatttc cttcatgact
     9421 ttttgtaaat cagcccaagc catttttttg gcagagggat attgcaataa gtaggaaggg
     9481 ctatagcttt ctaaaaaatt gatattgcca agattttgta tggaattctg cgtcggcttg
     9541 ctggaaaaca acatcacgaa aggggtggtt atcttttgtg tatattgttg cagaatttgc
     9601 gtcgtggatt tcgtatttcc cgcgagatca cattctaaaa tcaaatagcg acgattcccc
     9661 aggcgcatcg cttgaatcat tttatctaag agttctgcac actccggcgc aaaaatactt
     9721 tgatccgagg cttgtttaaa attcagaaaa agaagatcgc acttgtcagc ctgaatctca
     9781 gtttgaacat ttgctaggtt ctgacgaatg cgcgcggatt gaattgtttt cactcccagc
     9841 acgttcttca cataggaaaa ataattggtg atgctctctt ctgccgccat agctgagaat
     9901 ctattctttt cctggattaa agaccatgaa aaccaacttc ttgcattgcg ctattgccgg
     9961 gtttttaact cttaacccaa aactagaccg ctgacgtctc aagttaaggc acctcaagac
    10021 cgataggtct tttgagtgga tgttcttgtg aatgaacagg atgttctgaa gcaaaagcaa
    10081 agccgaaatt ccaattttag ctaggagggc ttttatggaa atcttccgtt ttaacgtggt
    10141 ccctgagcaa gaaatcaagc gtcgcgaaaa gttcaagtat gcactcagtc actgtgctgt
    10201 ttgccacgga aatctcgaat tcaattattt cgacaccctc gacgaagaca aagtcgaaga
    10261 ggtcgctcac tgcaaagatt gcggccgcaa ggccacgaac caactccatt cgattcattg
    10321 aagcgttcaa aaaacacaaa tgaccaagga taaaaccctg ttcatttgtg aacttattgg
    10381 tgaatttaga agttgtagtc agttataaag gacgtgtgac ctgcagaaaa gaatactttt
    10441 taaaatcgga atctctggtg aatatccgtt ttacattatt ttgtttaagg catgtggcaa
    10501 ttcttgcatc aaagatttca cttcccttca agccaggaat ttgcttttca atttcaggga
    10561 gcaatctcca ccaatttatg tcctcttcca gtattcgaat attataactc tctaatagat
    10621 ctgaaaagac tgaaatggct ttggcaattt ttagtggcga ttcaaatact tttggatgtg
    10681 tcaaaattcg aagagtttct cctacatttg taggagaaat acataagtca tcattgatgt
    10741 tcgtcaagcc tctcatagct tcttcatgaa atgaagacga agagaccata attcctataa
    10801 gaatatttgt atcaagtgcg gtcatcttca agatcctcca tccattcttt gcgggttgtc
    10861 agatcgattc gctcagcccc aagatccaag ggcttaaagc tctttacttt ttgacctttt
    10921 ttttgccgcc aaagatcccg acctagcacc gcccattgac ttacaatctc actgattgaa
    10981 cgcccacttt tttgagcttc ttttttggcc tcttcaaaaa ctttatcatc aagaataaga
    11041 ctggtcttca tgtaatctcc tatattctat attaaagaat atatattctt aaatcaataa
    11101 aataggatgc ctgtttcaaa atgaatcact aaagaaagcc gacttcagtt acctactact
    11161 atgcgctctc ataacctaga aacgaattct cacgccaatc ccggcatcaa gatccgccgc
    11221 tattgaaggg ataagttcta atatcatcgc cagttctccg aagaattcaa ggtttggatt
    11281 attgatatta aaatcaagtc ctaaagatcc gcgaggaccg agagagattt gagagttgtc
    11341 ttctctgtcg ttataagtaa tcaaacgacc tcccaacccg taatacatat ttaaaggacc
    11401 ttgagtggtt ccccaactgc gcgcgcgatc ccataaataa tccatatgca attgcagtcc
    11461 gctatgtctt ccactggaat aagcaagagc gccatcaatg gcatgacgct catccaattt
    11521 cgctttcgca gaaagtcctg taggatctcc caagataatg cccaatccaa agttttccgc
    11581 gtgggccagc ggcgctttaa aaactcctat aaaaaccgtg gttaaaaata tgtaaatcca
    11641 tgcgcgcatt ttcatagttg tgactcctca ttttttactt tagttttcat tttagtttta
    11701 tcttcagctt attttttact ttatctataa gtgaaactgc ctgctccacc ttatacagat
    11761 agccgatgcc aaaatacaat aacccgtaaa cccccaaaac aatcatcgcg cgtaaaataa
    11821 catgtgcaaa attattcaat aggaacttta ttccaaaagc aagagcggcg ctgacgatag
    11881 ccactcccca aagcttgagt tgaaaggaaa acttcaaacc ggtgtcaccg attttcttgt
    11941 tgagggtgtg gcgaagtaaa taaaattcaa tccagcctgc gaggcccgct gaagccgtca
    12001 gcccggtggt tccccaccgc aaatcaacac ccaaaagttt tggcaaataa aaagcacaga
    12061 acacacccaa aattgtggtg aagaaaactc gcaccaaggc aaattttaaa ggcgtccgcg
    12121 tatcacgcaa ggaataaaaa gttgaggagt aaagcctgcc taaagtacta gccaaaagtc
    12181 ctactgtaga cccggctaaa accgcccaaa cataattcgt actgtcgtga tcaaaagcac
    12241 ccgtctgaaa aattgctccg acgatcaaat cccccaaagc taaaaaaccc accacagagg
    12301 gaataataaa aaaagcgatc tgctgaagcc ccccgttcag tcgcgtttgt aaataaagac
    12361 tgacttcttg atctgtcccc gtcgcctggg acatggcggg aagttcgacc accgaaatgc
    12421 tcattccgaa aagactgatc ggcagcaaat acaaagtttg agcataggcc aacgcagaaa
    12481 ccgccccagt cggcagaaga cttgccaaca cgttatcgat ataagcactg acttgcacca
    12541 ctcctcgaga aaagaccacg gggaaaaagt ttttcagaac agttctgaca ctctcaactt
    12601 ttaaattcag gctgggataa attttccgtc ccaatttcaa ggcaaaagga aattgaatga
    12661 gaaattgcaa aaagcttccc agcaccaagc cgtaggatgc ggtgatcgcg agcggatacg
    12721 gagcttgatg gcgccctcct aaaatagcgc caataatcgc caaattccaa atcaccgggg
    12781 ctacatagga aagaaaaaat tttctatgag aattcaaaat ccctaagcac caagctgaca
    12841 tcaccagaaa accagttccg ggaaaaagaa tttgcacaat ctcaatggtc agctgtcttt
    12901 tttcgccgtg aaaacctggc gcgatcacat caattaaaaa gggcgtggca aaaaccccga
    12961 taagaaccag aaccgatgtg gctacaaata gtaaacttcc cacgactgaa gccaccttgg
    13021 cggcctcttc ctcgtgtttt ttcgctagaa gttgagcgta aaccggaata aaactggctg
    13081 acagaacccc ttcgccaaaa agattctgca agaagttggg aattttcaaa gccgccttaa
    13141 aggcatctcc ggcgtcggaa tttccaaagt aatgggcgaa aactctttca cgaaccaggc
    13201 ccgctatgcg gctaaaaaat atccctaatc ccaccaaaaa ggcatgcttc ttcatgaatc
    13261 ctcatcttca ctttcattca actgtaaaag cacacgctcg gcaagcactc tgttaaaccc
    13321 ctttagcttt gcgatctctt caggatccgc ttccttcaac tcctgcacac ccttgaaatg
    13381 ggtgagcagg actttctttc ttttttcacc tagacctatc acgaaatcaa gttcactctc
    13441 caggctggta ttttcgcgca atttgcgatg gtaagtaata gcgaagcgat gggcctcatc
    13501 gcgtatgccc actaaaatgt gcaaagcctc tgagttcgaa cgaaaggtca gagcattttg
    13561 acgcccaggc aaaaagaaac gctcttcagt gcttgaaact tctgaagatt gaaaatccga
    13621 ctcggttcga gccttcgcca accccaccac gggaatctct ttacggccaa tctcctctaa
    13681 gattctcatg gcttgagaca attgcccttt tcccccatcc accaccaaca aatgagggtc
    13741 ttcatattca gtgtgcttaa agcgacggct cagaacctca tacatggagg caaaatcatt
    13801 ggagccttcc accgttttaa ttttatagcg acggtaattt tctttactgg ggactccctc
    13861 ttcaaagacc acttgcgaag ccacggtctc cgtgccctga aaattcgaaa tatcatagca
    13921 ttctatccgt gtcggccgct gactcaagcc aaaacgctct ttgatttcat cgagaccccg
    13981 cattttctct tcggatttag aaacatattt ttcaaaatgc gctttggcat tctgatgcgc
    14041 catttcgacc aaagaccgac ctttttcatc cgttgcaaag cgcaccacgc ttttatgccc
    14101 tgaacgctcc aataaaaccg cttccattaa cttgttcaga tcgccgccaa tttccgtcgg
    14161 caagagcact tcgtcgggaa tgatattgtc ctcgtagtat tgattcataa aagaaaccag
    14221 ccactctcgg gcatcgctgg ccggatccac cacatccaga accgaaataa aatgagaacg
    14281 agttccaatc acccgtccct ggcgaacgtg cactgtttca ataagagttc cgcgatcatc
    14341 cccaaagtaa ccaatcgcat cttgatcttt ttcagaagta tcattgatca ccgattgctt
    14401 ttccaggatc gatttcacgc tctcgataga atcccggagc cttgcagcca cttcgaattt
    14461 ttcttcctcc gccgctagca gcattttctg cttcagggtt ttaagcactg ttttattttt
    14521 tcctttcaaa aataacaacg ccccttccac ctctgaacga tactcttcgc tggaaatata
    14581 ttccacacag ggagcggtac agcggccaat ttgatgggtc atgcaaggac gtttcctggc
    14641 tttcaacatg gcatccgtgc aatcacgaat tttaaaggtg cgatttagaa accgaatagt
    14701 cccaaagacc gcgccgccgc tggtgtaggg accaaaataa agactgccat cttttttcac
    14761 cttacgactg agatacaaac gagggtaatc ctcgttccag gtgaaacgaa tataggggta
    14821 agccttatca tcccgcagac gaatgttgta ctttggacgg tgctttttaa taagcgaggc
    14881 ttccagcaaa aaagcctcca cttcagtttt cgtaagaatg tattcgatgt gagtgatgtt
    14941 ttgcacaagc aaacgggttt ttggagtatg atctttgctg tcagtaaagt aactgcgcac
    15001 ccgatttctg aggttcttgg cttttcccac gtagatgatc ttatcctgag cgctcttcat
    15061 cagatagacg ccactttgaa taggatatcc cttgattaac tctcggagct cgtcaaactt
    15121 ttgtgacata ttctacttct tcagcccctc aatgatcgtt tcggtttctt tctccacttc
    15181 gccgcttctt aagctgagat ccaaaatttg caatcgtttg atctcatcac ggattttagc
    15241 ggcttcctca aactccagat ccgaagagtg ttttttcatt tttttgcgca gtttttcaat
    15301 ttcttcctga atcttgttgg gctcattgcc aaacttctgc gccatgcccg cctgttttct
    15361 ttccattcca tcgaccagat gagcgcccac cgagccgtca aaaacatcgc ccaatccttc
    15421 tttaatacgt ttcttcaccg aggccggagt gatcccgtat ttaagattat gctcggtctg
    15481 aatacgacga cggcgatcgg tttcgtcgat ggcctttctc atcgagtccg tgatacgatc
    15541 cccgtaaaga atcactttgc cattgagatt tcgcgccgca cggccaatgg tttgaatcaa
    15601 agatctttgc gaacgtaaaa aaccctcttt atccgcatcg gtgatcccca ctaagctgac
    15661 ctcgggaata tccaaacctt cgcgcagcaa attgatgccg acaagaacat cgaaaactcc
    15721 gagccgcagg tcgcgcagaa tctcggtccg ctccacggtt tgaatttcac tgtgaagata
    15781 cttcaccttc actcccaagc tttcataata ctccgtcaaa tcttcagcac ttcgcttggt
    15841 caaagtcgta atcaaaaccc gatccccgtt tttaacccgc tcgcgaattt ccgccaacaa
    15901 atcatcgacc tgatgacgaa ccgggcgcac ttcgaccaca ggatcgatca agcccgtggg
    15961 acgaatgatt tgctcaatga tcacgccctc ggacttttca aactcataat ttccgggagt
    16021 ggccgacaca tacaccactt tatccatcat gcgctcaaac tcttgaaaat tcagcgggcg
    16081 attgtccaat gcgctcggca agcgaaagcc atgttcgaca agagtggtct tcctggctct
    16141 gtccccccga tacatcccgc caatttgcgg aaccgtcaca tgggactcat caataaacgt
    16201 gacaaagtgc tttggaaagt attccaacag agtcggagga ggctcccccg gcccacgccc
    16261 cgtcatatgg cgggagtaat tttcgattcc ctgacaaaat cccatttgct cgatcatctc
    16321 aatgtcgtaa taagttcttt gctccagacg ttgcgcttct aacaacttca cattgttttt
    16381 taaagccacc aattgttcgc gcagctcttc ttgaatggtt ttaatggcac gattgagatt
    16441 ctcatctccg gtcacgtgat ggcttccggg gtaaataccg atttgatcca gctcttcaag
    16501 aacttggccc gtcaatggat cgacccagga aatgcgttcc acgtagtccc cgaaaaactc
    16561 cacccgaatc gcccgctcct cttcgtaaga ggggaaaacc tcaaccacat cgccgcgcac
    16621 tcgcaccgtc ccacgatgaa aatccacatt atttctggtg tactgaatgc gaatcagttc
    16681 gtgcaaaaaa tgatcgcgct tcaattcaga attcgccacc aaatgaatca tcatcccttc
    16741 ataggcttcc ggcgagccca agccataaat gcaagaaacg gaactcacga tgatgacatc
    16801 ccggcgatca aataaagaac gcgtggcgga gtggcgcatg cgatcaatct gctcattgat
    16861 ggcggaatct ttttctatat aagtatctgt cgaaggaata taagcctcag gctgataata
    16921 atcatagtag ctgacaaagt attccaccgc atttttcgga aagagttcct taaattccgc
    16981 ataaagctgg gcagccaggg ttttattcgg agccaacacc aaagcgggct cattcaactg
    17041 agcaatcgta tgcgccatgg taaaggtctt ccctgaaccc gtgacaccta acaaagtctg
    17101 atgattaagc cctagcttaa aattctgcat caaatcctcg atggccttcg gctggtcccc
    17161 cgaaggcttg aattctgaaa ccaattcaaa gctctttttg actgccataa acacctgcct
    17221 ccacttaaaa caggggaatt gaaatccctt atcttaaatg cttctaaggc tggtgtgaat
    17281 attttgtttc gaaatgcgcc aaatgggata cagcgccgaa agaatgtttc ctccgatcac
    17341 ccaccaaatt gagttcattt cccagccaag ctttagctcc agttttttta gccagagaaa
    17401 agcctcccac aatggggcca atactgtatg cataagacag actagataga agtgtcatca
    17461 tagcaagagc ccacagcgag catctgaata aacattactg actctctttt ctctctgttg
    17521 aggggatctt tctaaatatc gtgggttttg tgtggaaaac cctaactgcc atcgtggcag
    17581 atgaagaccg cccgcccact ccggggcaag tctggtcatc ccgatcagac ttcttacatt
    17641 tatttttacc aaggtctcct gaacacgcgt tgaataatca tcttcgacaa aaattccaga
    17701 aattttaagg ggtttttcaa ggaacactca ttgaagccaa aaatgcgttt tgtccaacaa
    17761 gtcagaaggc tgtaacagaa aaaattgtgc gttcgaaata ttttgaactc ttcaaaagcg
    17821 cgagaaccgc ttatattact tatgaaattt aaattcgaga ttaaattctt ggcttaaagc
    17881 ttaaactcca atggtgttag aattattaca gtatgtacga cagctgaatt gtgttatcat
    17941 ctggccgaaa tttatttttt ctgataacaa tttaattgga ggattcatga aatcattatt
    18001 ttttatttta atagcagctc tttcagcttt tgcaaacgca ggtgaacttg atggaatgac
    18061 tttttgtaga aaagttgaaa caggtggtat gtttggtcaa ccaaaaggta ctcgagagca
    18121 ctgtgtttcc tttaaagaag atgtaatgac agacaatgca aatacttttt ttggaaatcc
    18181 gcccgagtgt ttttcttaca ctatcgaaaa ctctaaaata attaattctg ataaaaactc
    18241 cgatacaggg tatgagattc agggagaggc tatagtagtc ccagaaactg gcgctgtttt
    18301 aactcttaaa ttaaatagat gatgtttcaa aatcactcct ctttgaatcc gagctcacat
    18361 tacgtgactg tcatttagat ttatttttaa aatcggctgc cacggcatag ctctccacga
    18421 acggctatgg ctgccaacct acttcgtcac ttcccaaaaa ctgccctcag gagtatcgct
    18481 cacagaaatc cccagctccg tgagcttctt gcgatactca tcggatttcg caaagtcttt
    18541 atcggcgcgg gcttgagagc gctccgccac cagttgatcc accgccgccc gctgcagatt
    18601 cattttctcc agcaacatat tatcgagctt caataaaaag ccattcgcag gttcttggaa
    18661 catcgccatg agagcaccca gtttgcgcac aaaattaaga aagctctgtg ccttaccaag
    18721 tactgcgggg ttgggcttga ttccacgctt cacctgagta ttaaactgac gaaccacttc
    18781 aaaaagtgcc gccatcgcat tcggagtgcc aaagtcatca ttcaaggctt cactcacctt
    18841 tgtccaagcc tcagcggtga tcttctcaaa acccacatct acagggactg tcgcctcccc
    18901 aagcaaactc tcagccacgg ctgttgccga gtaaatgcgt gccagattgg agaccgcccg
    18961 atccacggcc tcgtcactaa agtcactggg cgaacgatag tgcaccgaca agatcatcca
    19021 tttataaatt tcagaattat atttctctaa aaactctcgc atggtcacaa agttgccaag
    19081 ggatttggac atcttctgat tgccgaagtt gagcatattc caatgcatcc agtattttac
    19141 aaaatgcttc ccgctgcagc cttcactttg tgctatttca ttttcatgat gcggaaaaat
    19201 caaatccgtt ccgcccccat gaatatcgat ttgctcgccg aagatttttt caatcatcgc
    19261 cgagcattca atatgccaac ctggacggcc agggccccag ggagacggcc acgaaggctc
    19321 tccgggcttc gcccctttcc acagagcgaa atcaagcgga ctttttttct tttcatcaat
    19381 ctcaacccgc gcgcccgctt taaggtcttc cacctggcgg ccactcagtt ttccgtagct
    19441 cgaaaaactt tcgatggaat aaagaacatc cccatccgcc acataagctt ttttattttc
    19501 tatcaactga gccacaatgg agcggatttc atccatgctc tcagtcacct ttgggttatg
    19561 gtcatgggga cgaagtccca gaagggaaaa atcttttttg tactcagcaa tatatttctc
    19621 agccaaatct tcaggcttca caccctgctt gttggcgctt tcaataattt tatcatccac
    19681 atcggtaaag ttgagagcat attccacttg atagcctaaa ttctccaacc aattgcgtac
    19741 cagattaaaa aacacgaccc cgcgaaaatt tcccacgtgc agaaaactgt aaactgtcgg
    19801 accacaaaca taaatcttca ctttgccttt tcctaaaggt tcaaagtcct ctaactttct
    19861 agattctgta ttgtgtatac gcagcatttt ttatccgatc ttagctaaaa ctttattcac
    19921 gcgatcgacc aacgaggcgg tcttaatcag aggaaccaat atcttcagct cggttccgtg
    19981 aggctttcca atcaccgcca cccggatcgg cataaagaga tttttccctt tagctcctgt
    20041 tttattttta acttcatctt gaagtttcaa gaattcctct tcagataagt aatcttttcc
    20101 tgaagcttga accaattctt tccaggtcgt caacacggct ttcgtcggct cccaagctaa
    20161 agcctcgtca gcctctggct gaatcacaaa agaattgtca ttcaaaggct tgtacagctc
    20221 aatggcatcc gccaaagttt ccatagaagt cttaaaggcc acaatggact tttcttgcca
    20281 cataggatcc tgcggaagct gcatcttctc gcgagccaga aaaggctgaa tctctttcca
    20341 aagttccata ttaggcaaag cccgtaagtg catggcattc atccacttga atttcacccg
    20401 atcaaaaacg gcgcctgaag gattcaaacg agtgacatca aaggcctgaa tcatctcatc
    20461 caccgtcata atctctttgc cctcaggatg tgaccatccc aaaagagcga tgaaatttaa
    20521 aacggctgaa ggcaaataac cttcttcttt cagctgaccg caagccacgg cacccttgcg
    20581 cttggagagc ttctgccgat cctcatccag aattaaagag atgtgggcaa actgcggagc
    20641 ctgccacccc atggcttcgt aaatcatcag ttggcgaaga gtgttgggca aatgttcttc
    20701 agcccggaac acatggctca ttttcattag gtggtcatcg atgacgcagc agaaattata
    20761 aaccggcatc cctccggaac ggagaatgac aaaatccccc accatatctg aggggaactt
    20821 tacttcgccg cggacaaggt cggtgaaaat ataatctttt tttagctccc gggttttaaa
    20881 gcgcaccacg gctttttctc ccgcggctaa tttttcttgg gccttcgcca aaggccaatc
    20941 ttggtaagga gactccactt gcggaggacg tcccgcctga atagcggcct cacgttgtac
    21001 ggtgatttcc tcatccgtta agaagcaata ataagctttg ccggagttca atagctcgtt
    21061 ggcaatagct ttataaatag gaagacgctc gctttgcttg taaggaccta agggcccctg
    21121 atcctgcaaa gtgaccgggt ccacgccctc tgcccaaaga aggttcaacc agaccatgtc
    21181 atccaccact ccacgcaaag attcctgggt ggagcgcgcc tcgtccgtgt cttcaatgcg
    21241 aagaatgaat tcgcccctct gttttttggc gaaaagataa ttgtacaaag ccgtacgggc
    21301 ccctccaaca tgaagatagc cagtgggaga cggagcaaag cgaacacgaa ccttagacat
    21361 aagaacctct aaaaagaaaa aagcccggga aaaacccagg cttttaataa tcgctaattt
    21421 tattagaaaa atcaaaccaa gttaacttac ggaactactc caaaaatcgc tttcacttct
    21481 tcctctttaa aaagctcttc ttgactcgtc gctaaggtca gctccttcag aagaagagtg
    21541 cgtgccgtat caagcatttt acgttcgcca aagctcaact ctttatcaac ctttagtaaa
    21601 aacaaatcgc gtaaaacttc agcgatttcg aaaacagaac cagttttaat cttttccatg
    21661 tattcacggt agcggcgatt ccaagtttga gtatcaattt taacatcagt ttcacgcaga
    21721 atgcttacta cacgacccgc ttcttcttta gaaataatcg gacgaagacc tacagattca
    21781 acgttatccc ggggaatcat aatcttcatc ccagtatcga gaatttggat agaataaaag
    21841 gtctgcttat tgccgaggat ctcttttgtt tcaattgctg tgaccttgcc tacgccatgg
    21901 cccggataca ccgcattgtc accaacatta aaagtcacca tcggaagacc tccctcgttt
    21961 gaccctaacc caataagagc cttggtgttt ttagcaaatt tgacacgagt tatcaaatga
    22021 aagcgactta gctagaaaaa atacaaaaaa gccccgcttg cggcgaggcc ttcatcaagt
    22081 aatatcaata acttacaaat tagttctcac gcgctgtata aactaatgca taaggctgtc
    22141 tgccgttttg accctgggga actttaggag ctttcaccgt aagggtgtag gaacccgctg
    22201 gcaaattgct cagctcaata acttcattgt tgttcacgtg atcgctcgga gccacattct
    22261 gaccattgcc agtcaaagtg agatccaagt cgttgaccaa tgccaccgct gcgtccggag
    22321 aagctggagc atccgtataa accatattca ctaagaggga accagatccg ttcaagatga
    22381 cggtgaaaga gtgttcttcg ccttgagcta aaccactttt atcgtccaca aaatgagtat
    22441 tgctagaaag attggccgca ctgcccacat ccatacgtcc atagccttca tcagagttcg
    22501 gacgacgagt cgcaagttct tgacctcgag ctacgcccac ttctccgtac tggccaggga
    22561 acatatcgat tgccgtgtgc aaaagagtcg cttttaatag agccgctgaa ggagaggcca
    22621 gttgaaactt ctcttgcaat acttgacgaa ccactgcagc cccgccagcg accagtggag
    22681 tcgccatgga agtaccccca gagtacgcat aatccacatt gtacgcgccc caaagaggac
    22741 tcgcacccac tacatgagaa cgattgctca agatgttggt tcctggagct acgatttcag
    22801 gttttgtacg accatccact gtcgggccac gagatgagaa catcgccaaa ccgttgatgt
    22861 tgtcagagat gtaagagcta gcaataggct ccgcactcca gtgatcggca gcggagcgaa
    22921 gcttgttgat aggagactgg ataccgcccg tgcttatcaa gttttctgaa gctcctactg
    22981 tcaaagcatt ttttgacgta ccgggggaac tcacagagtt gggatcaatg cgaccgtctt
    23041 tgttcgcatc aattccgcta ttgccagccg cgaaaatgat caacatatcc ggattattga
    23101 acgtaaattc gtccaagctc gaagcgtagt tgtcataagc cccgaagttt cgcgcagacc
    23161 cccaagagtt cgagtgaacg cgggctccat cggcataggc tttagagaag agatcttgaa
    23221 tcttagaggg caccgtgagg tttttcatca tcggagacca catggcctca gggatgagac
    23281 tcgcctcata ggcaccccca cgaatttttc cgccggaagc agtcccgcga ccaagaatag
    23341 aaccggccac atgagttcca tgccccatag gatcatccca agctttagag aaaagaccga
    23401 agatataacc tttaagaacc gcgtctttaa aatctccggt taattggttt tcacccatat
    23461 ccagacctgt gtcagccatg gccaccactt gattgcgccc tttatagccc atcgcccacg
    23521 ctggatcgaa gttggctact ttggttccag tctcataatc gttaagatcc gtataatcac
    23581 cagtcccttg aactatagga gttggcgttc cctcgtcaaa aaccatgtgc atggatttca
    23641 tttcaacata gggctgtatg tgctcaacac cttgaaaagc ggccaccgca cgaaccattc
    23701 cgcgaggaac cactgcgacg atggacttgc cgtccgcata gtgaactgtg gattgagcca
    23761 agcttttgat cttcactgcg atgtctctgg tctcattcgc tttgaaagtt tttactaaaa
    23821 tagaagtctg ctcttcttta ttaaaaatgc tggccgcacc aaaagaagtg ctgactttat
    23881 aatctgcttt gaaaggaaga accgcttcga tcgccttatg agtgcgccat tgctggatct
    23941 ttgcaatagg agcccgaacc attaaagcat cgtccggaag ataaccaaaa atctccgccc
    24001 ccattgcttt cagttccgct ttgtcttttt ccgtgatcag gtttttaaac tgaatgatca
    24061 aatcttgagg ccctgatagg ttgcccgctt gagcactcat caggctctct acttgcttgg
    24121 actccaccct cccgacattc aaccgcagca ctgtgcctgc gtgagctaaa gaagatatcg
    24181 acgcagcaat agatgcaata actaaaatgt tctttgaaag taaattcact ttcccctccc
    24241 ccactaaagt ttcgaaaatc agatcataca gtcgaagggg tgtatagaaa aaaataaatg
    24301 cactgctgca ttaggcaatg cctgcaagat tggcggggcc atgagttttc gtgagttctt
    24361 cacgcaaaga ctggttttct gcaagtttta actgattgtc ttttcggaga acctcgaaag
    24421 cggcttcacg agcttcttga agaatcgaca tatctctcac caaattagcc attttaaatc
    24481 caggcaaacc ggattgacga gtgcccataa attctccggg tccacgcatt tccaaatcga
    24541 attcggcgat cttaaaacca tcagtggttt tttccataaa ctccgtgcgc agcttggctt
    24601 cttcggaaac cgcgtaaccc ataataagaa tgcaataact tttatgagat ccgcgtccga
    24661 ctcttcctct taattggtgc aactgagaca acccgaaacg ttccgcatgt tcaataatca
    24721 tcatattggc attgggaaca tccactccca cttcaatcac tgtcgtggaa accaaaacct
    24781 gcaaatcacc cttgcggaaa tgcgtcatca cttcttcttt ttcttgaggc ttcattttcc
    24841 catgcaaaag acccattttt atgttgggaa attgccgctg aaggttttgg aattcagaaa
    24901 ccgcattttt taaatctatt ttttcgcttt cttcaaccaa ggggtaaacg atataagcct
    24961 gtcgcccctt attgatttgc tcttgcatga actgcaaagc tgcgtttttt ttactatcaa
    25021 aaatcgcccg ggtttggatc ggacttcttc ccggcggcat ttcatcaata atggaaacat
    25081 ctaaatcccc atacaccgtc atagctaagg ttcgaggaat gggggtcgca gtcatcacca
    25141 gaaagtgggg agagaggcct ttgcctttta aggctccgcg ctgctccact ccaaaacgat
    25201 gctgctcatc aataataacc aaagccaaac tcttaaactg aacttcctct tcgaggaggg
    25261 catgagtgcc catgaggaaa tcaatttcgc caaccgccag ttgctgcaaa agctgctttc
    25321 gttccgccgt ctttgttttt cccgtgagga ggcccaaacg aagtcccagc ggcgccagca
    25381 attgctgggc atttttgaaa tgctgttcgg ccaagatctc cgtgggcacc atcaagcaac
    25441 tttgcaaacc gctttcactg gcagcaactg ccgccataaa acacaccaag gtttttccgg
    25501 aacccacatc gccttggacc atgcgatgca tgggagagga tttttccaaa tctgttttaa
    25561 tttcttgaaa agcttttttt tgcgcattgg ttaagctaaa gggcaaagat ttttgcagtt
    25621 cttcgagctt ttttccatcg ctggaaaaag ccaggccttt ctctttttca aagccgattt
    25681 ttttcgaagc caaataaagt tctaaccaaa aaaactcttc aaagatggtg cgacgatgtg
    25741 ccggggtttt aagctccgca tattggaccc ctaacgccgt ctccggatca tgaagcagct
    25801 ttaaagcttt tcgacgagag atcaaattgt acttttttac gatccaagac ggcaattgct
    25861 ccggcggcca ctcctgttcc ggaagcaaag acaaagcggc tttaataaaa cgatggattt
    25921 tggcggtgcc caagccctca atctcggtat aaagaggaat caaagaatcg tgattttcct
    25981 cttcgggatc gatgtcgcgg atatcgggat gttggaattg aatcttgccc cggtaatcac
    26041 tgactttacc gacgactcgg acttctttaa aaggcgtaaa gcgctcaaaa tagcccttgt
    26101 aaggaatgcg aaaaaatttg cagcgaattt gtcccgagga atcgcggacc acgacgtcat
    26161 acattttacg atgggaattc cccatattaa tactgtgaac agaaaccact tgcgctttta
    26221 cactgacgat atccccgact ttgaggctgg cgatattgcg agccgcgcgt tggtcttcat
    26281 aagcgcgcgg atagaactcc aaaagttcac ccactgttcg aatgccatgc cttgcaaaga
    26341 gcgaggccag cttgggaccg actcccttga gatattgtaa tgaggtatct agacgaaggg
    26401 ccatgttgat caaattttca tcttttgatt ttcgagtcaa tgaatccatg aaataatagg
    26461 gagatgcagc aggaagctta ttttcgaata cgtctgagca caatccgtcc gtcaaaagct
    26521 acatcttttg atatttacat tcaaatcaat ggccaaatgg ttctttatct taagacaggg
    26581 gacgcccttt ctagtttgaa gattgaatct ttgcataaca aggacacggg caatactttt
    26641 tttgttttga cctcgcaaaa acaagcctat cgggattggg tcaaagagga aatgaactcg
    26701 gcggagctca atccctttga aaaaagcaaa attttacgcg aatcctccgt cgccttgatg
    26761 gaagatctct ttgaaaaccc cgacgttaac accgcgctgg atgagtcgcg ccccattatt
    26821 aagaacttta tcgactttat ggaacaagct cctgaggcca tgggttatat gatcagcctt
    26881 tcgggacatg atttttatac ctataatcat tctttggacg tgagtattta ctccttgggg
    26941 ctgggctcga ttctttcctt caataagacg gaccttgagg atcttggcgt cagctctttg
    27001 tttcacgaca ttggcaagcg caacgtgagc ttagatattc tttgtaaaaa agggggcttg
    27061 gacgacgctg agtgggaaca aatgaaacgc caccctcagt atggactgat gatcctcaat
    27121 cagaatccaa atattagtga tgcgatcaag gccgcttgct ttgaacatca cgaaagcttc
    27181 actggaaatg gatatccgca agggatttct ggtcaagaaa ttcatccctt cgcacgtatt
    27241 gtcgccatca ctgacaccta cgatgcaatg acgactcagc gatcttataa tgcacccctt
    27301 aagccttttg atgctgtcat tatgatgaag gaaaagttgg ccggccgtta tgatcctgaa
    27361 tttttaaagg cgatgtattc agtattattt aaacttaagg gcgccgcata gcctgggaat
    27421 attatgctaa aacttctctt gaacacctat ctggcttttt tactcattcc cctgctcact
    27481 ctcgctatag aacccaccaa tccgacaggg ttatgcgatc gttttctggg ggaccaagag
    27541 aagaccgctt gtatggtaaa aacttcccaa accgaattgg attggtacgc ttcctcttcc
    27601 tgtgctcttc aacaagatga caagaacttc atgaattgtc ttgaggaaat caaaggcgcg
    27661 agcttcagtc ccgaagccct tgaactctgc gcaaaatctg tagatctcac ggatgaggct
    27721 cgcttgagct gtataaaaaa aattagaaat aaagactaca cccgtagcca aatcaaaaaa
    27781 tgctccgaag caggagttcc cgtggccgtc gagaattgcc tttctaacag cagccgtact
    27841 ccggccactg caggagtgac caaatcagga tttcagaccc tagaaatcaa aaaataagtt
    27901 ccatgg
//
LOCUS       DATBTE010000045         5329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_346811, whole genome shotgun sequence.
ACCESSION   DATBTE010000045 DATBTE010000000
VERSION     DATBTE010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5329
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346811"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(359..904)
                     /locus_tag="VIG33_01820"
     CDS             complement(359..904)
                     /locus_tag="VIG33_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY8269599.1"
                     /translation="MRMLSPISTDWSWPRRSFSSDRMEDVFEDFDRMVDRLFQPPTAD
                     TMHFQPCCDVNETKDHFLVSFDVPGVKKEDIKIEIQGNQLIISGERHYEMKEEKGEEA
                     LRRERSYGKFERTFVLPATISADKIEAHYENGVLNVALPKADAAKARTIQIQTDQDGF
                     FRRLLRVKEEDAKELKDVKIS"
     gene            1291..3567
                     /locus_tag="VIG33_01825"
     CDS             1291..3567
                     /locus_tag="VIG33_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEY8269600.1"
                     /translation="MTSSYQIDPVCGMTVSTENPKGGSSTFEGKTYYFCSPKCKAKFD
                     NNPSFYLQPKPKEVEPLNVEYTCPMHPEIRQMGPGSCPICGMALEPVTMILDHAEDTA
                     EYVDMKKRFWISTSLCIPLLFITMGGRHLFESELLHSFFNYAELALATPVVLWGGWPF
                     FERFLQSLKNKSPNMFTLIGLGVGVAYVFSVIALFFSDIFPGQIGLYFEPAAVIITLV
                     LLGQVLELKARSQTGDAIKALLGLSPKTARRIKADHSEEEVPLEEIQVGDLIRVRPGE
                     KIPVDGIVQSGTSSVDESMVTGESVPVEKSLGAKVVGATMNGTGSLVLKAEKVGRDTL
                     LSQIIQMVAEAQRSRAPIQKLADQVSGYFVPFVVLTALLTAVAWSIWGPEPRWTFAIV
                     NSVAVLIIACPCALGLATPMSIMVATGRAAKMGVLFRDAEAIELLRKVNVLIVDKTGT
                     LTEGKPRLITVKSFNDITEENLLQWTASVERQSEHPLAEAIVKGAHERNIELKAVESF
                     SSITGKGATATLLGFPVAVGNKSLMDDLGVSLADAEPEADLLRKQGQTVMFVSLDNKL
                     CGFIGVADPIKKTTVEAISELKKSGLRIVMVTGDNARTAQAVSAKVNVDEVVADVLPH
                     QKVEIVKKYQSQGFTVAMAGDGINDAPALAQAQVGIAMGTGTDVAMKSAGVTLVKGDL
                     QGIVRAHELSLKTIANIKQNLFFAFIYNSLGIPVAAGLLYPFTGLLLNPMFAAAAMSL
                     SSVSVIGNALRLRKTALK"
     gene            complement(3596..4465)
                     /locus_tag="VIG33_01830"
     CDS             complement(3596..4465)
                     /locus_tag="VIG33_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY8269601.1"
                     /translation="MLDVSLALGFISSAVIIFGGYYILKSITTIVEDESETFVLNCGQ
                     VTKRLQKPGLHWVPEKIFPWIKTFSVSKQVDFRTYKGIQVNDHFGTTVIVDLWVEFKI
                     VDPYKAIFCVENWEEVLQSLVTHTLASVLCSQTVEEILIHRTELAQSMHKVMGHQTER
                     WGVSIQGAMIQNLGLLPETSKQFFNSVAARIEKTKALIEEEGRLQVARLDAETTYRIA
                     ELSAVAKAQMAYEIGSAYRDLARRPQLLRAFQDYWEVVQTDPRKTITFSGFENSRVAP
                     LEASMAADSVLNH"
     gene            complement(4459..>5329)
                     /locus_tag="VIG33_01835"
     CDS             complement(4459..>5329)
                     /locus_tag="VIG33_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY8269602.1"
                     /translation="VIWAWNSVFKVEEGHAAVLTRFGAVVYEDEESTSDVNPLDFIDH
                     RRVKTFKPGVHWIWPWEKVHCFSTMERVLDLSGPDGGRHAMTADGTILRIDSKIRFKP
                     LEKHLYAYMFGLKNPMEHIKELFTCLIRNEIANFCNSKVDGDSREFVGSYAEIRRDRN
                     HFNFLIENFCQKQIGSSYGVQFNAVDLIDILPPPELEKALNGIQSATTEAKTIYSRAE
                     ADSRQRIAAAEQGVEIAKTRAQAVAVEIQTIAGVLEKLLDQGSLNYYLQHRRTELMTD
                     SRLTFVKKGELPC"
BASE COUNT         1466 a         1249 c         1106 g         1508 t
ORIGIN      
        1 catagggtcc ctcctatctc catgttttaa acaattaatt tgttggactg atcaatttat
       61 taccgaagcg gattcggtgg aactcctgag ggaactctca ctgtgtacct cctttaatac
      121 gtccagggca cgaattagat aaagaagtta gagccttatg ctttagccct gatatttcta
      181 atataatttt cggatcaggt aaaaaaatgt caagagacga aaattgattt tttcaaaaat
      241 ctggactcct taaaatttct ttgagatatc aagactttcg ctaaagcatt ttgagtttta
      301 ttaaaaaaaa aggtgaccgt ttcattacga aacaatcacc ccctcgtgat cttggatgtc
      361 aggagatctt cacgtctttc aattctttcg catcctcttc tttgaccctc aacagtctgc
      421 ggaagaagcc gtcttggtca gtttgaattt ggatagttcg cgcttttgct gcatcggctt
      481 ttggaagagc cacgttcaag accccgttct cataatgagc ttctatttta tcagcactaa
      541 ttgttgctgg cagtacaaag gtccgttcaa attttccata gcttcgctca cgacgaagcg
      601 cttcctcgcc cttttcttct ttcatttcat aatggcgttc tccggaaata atcaattgat
      661 ttccttggat ctcaattttg atgtcctctt tcttaactcc gggcacatca aagctgacca
      721 ggaaatgatc ctttgtttca ttcacatcac aacagggctg aaagtgcatg gtgtccgctg
      781 ttggtggctg aaacaaccta tcaaccatgc gatcaaaatc ttcaaagaca tcttccatgc
      841 ggtcggaaga gaagctgcga cgtggccatg accagtctgt tgaaattgga cttaacattc
      901 tcattgtgta cctcctatga atggcagttg tagattttat tttttctctc tttccaatta
      961 taaaatgaga ttacaaaaat aactgtcaag tgaggtcgta aaaatttttc ttcgagcgac
     1021 aaaaatagct caaagagttt caacaattta ttcgatggct tgacaaaact ttttgcgacc
     1081 tcatctttaa agaaaggggg tacgagccat gtcagtaaag aacttccact ctccaacaaa
     1141 gagaaatatt ttcgcctcta cgacagctat cgacacaaca aaaactggcg aaaaatgatc
     1201 agtaataatt aattgatacc tacccccagt aggtataata ataagtcata aatcctcccc
     1261 aagaatcgag cctgttatga gaggtgtatt atgacttcca gctaccagat tgatcccgtc
     1321 tgcggtatga cagtgtctac agaaaatcct aaggggggct ctagtacctt tgaaggcaaa
     1381 acctattatt tttgcagtcc taaatgcaaa gcgaagtttg acaataatcc ttctttttat
     1441 ctccagccaa agccaaagga agtggaacct ctgaacgtcg aatacacgtg tcccatgcac
     1501 cccgagattc gacagatggg accaggaagc tgtcctatct gtggcatggc tttagagcct
     1561 gtcacgatga tcctcgacca tgcggaagac accgcagaat atgtcgatat gaaaaaaagg
     1621 ttttggattt caacgagcct ttgcattcca ttgctgttca ttaccatggg cggtcgccat
     1681 ctttttgaat cagaacttct ccattctttt tttaattatg ctgaacttgc tctggcgact
     1741 cctgtggtgc tttggggtgg atggcctttt ttcgaacgat ttctgcaatc actaaaaaac
     1801 aaaagtccta atatgttcac cctcattggg ttgggcgttg gcgttgccta tgtcttcagt
     1861 gtcattgcgc tctttttttc cgacattttt cccggtcaga tcggcctcta ctttgaacct
     1921 gcggccgtga tcatcactct tgtccttctg ggacaagttt tggaacttaa agcacgcagc
     1981 caaactggtg atgccataaa ggcgcttctg ggcctttccc ctaaaacggc tcgcagaatt
     2041 aaagccgatc attcagagga agaagtccca cttgaagaaa ttcaagtggg tgatcttatc
     2101 cgagtccgtc ccggagaaaa aatacctgtc gatggcattg tgcaatcagg aacaagttct
     2161 gttgatgagt ccatggtgac aggtgaatct gttccggttg aaaaatctct gggcgcaaaa
     2221 gtggtaggcg ccacgatgaa tggaacaggt tctttagtct tgaaggccga gaaagtggga
     2281 cgcgatactc tgctttcaca aattattcag atggtggcgg aggcgcagag gtcccgggcc
     2341 cccattcaaa aactagcgga ccaagtctcg ggctactttg ttccctttgt ggttttgaca
     2401 gctcttctca ctgccgtggc ctggtctatt tggggaccag aacctaggtg gactttcgcc
     2461 attgtaaact ccgtcgctgt ccttattatc gcctgcccct gtgctttagg actcgccacg
     2521 cctatgtcca tcatggtggc caccgggcga gccgcaaaaa tgggagttct cttcagagac
     2581 gctgaagcca tcgaattgct gagaaaagta aacgtcttga tcgtagataa aacaggaacc
     2641 ctcacagaag gaaagcctcg ccttatcaca gtaaaatcct ttaacgatat cacggaagaa
     2701 aatctcctgc aatggaccgc cagtgtggaa cgccaaagcg agcatcccct ggccgaagcc
     2761 atcgttaagg gagctcacga gcgaaatata gaactcaaag ctgtcgaatc attttcttca
     2821 ataactggaa aaggtgcaac agccaccctc ttaggatttc cagtggccgt tggcaataaa
     2881 tcactaatgg acgatttagg tgtttcgctt gcagacgctg agccagaagc ggatctgctg
     2941 cgcaagcaag gacaaacggt gatgtttgtg agcctagaca acaaactctg cggtttcatc
     3001 ggagttgcag atcccattaa aaagaccact gtcgaagcca tttcagaact caaaaaatcg
     3061 ggactcagaa tcgttatggt cacgggcgac aatgcacgaa cagctcaggc cgtctccgca
     3121 aaagtaaatg tcgatgaagt ggtggcggat gttctacccc atcaaaaagt ggagatcgta
     3181 aagaaatatc aatctcaagg atttaccgtt gccatggctg gtgatggaat caacgatgct
     3241 cctgctttag cccaagccca ggtcggtatt gccatgggca ccggtactga tgtggcgatg
     3301 aaaagcgctg gtgtgacgct ggtcaaaggg gatctgcaag gaattgtacg ggctcacgaa
     3361 ctgagcctta aaacaatagc caatattaaa cagaatttgt tcttcgcatt tatttacaac
     3421 tctctcggta ttccggtggc agcgggattg ctttatcctt ttaccggttt attattaaat
     3481 cccatgttcg ctgcggctgc catgagcctt agttctgttt cggtcattgg aaatgccttg
     3541 agactgagaa agacggcttt aaaatgaatc gcctttctca gaatcacttc ttcatttaat
     3601 ggttcaggac tgaatccgct gccatggaag cctctaaggg agccactcga gagttttcaa
     3661 atcccgaaaa ggtgatcgtc ttacgcggat ctgtttgaac aacttcccaa taatcttgga
     3721 aggctcgaag caattggggc cttctcgcga gatcccgata agcgctgcca atttcgtaag
     3781 ccatttgcgc tttagcaacc gcactcaatt ccgcgatccg ataggtcgtt tccgcatcca
     3841 gtcgggctac ttgcaatctt ccttcttcct caataagcgc cttcgttttt tcaattcgtg
     3901 ccgccacaga attaaaaaat tgttttgaag tctctggcaa aagtcctaag ttctggatca
     3961 ttgctccctg gatactcacc ccccaacgct ccgtttggtg acccattact ttatgcatgc
     4021 tttgcgccag ctcggtgcga tggataagta tttcttcaac tgtctgtgaa cacaagaccg
     4081 aagcaagagt atgggttact aaactttgta aaacttcttc ccaattttcg acgcaaaaaa
     4141 tcgccttata agggtctaca atcttgaatt caacccaaag atccacaatg acggtggttc
     4201 caaaatgatc gttaacttgg atccccttgt aggtcctaaa atctacttgc ttggacacag
     4261 aaaatgtttt tatccaagga aatatttttt caggaaccca atgtaaccca ggcttctgca
     4321 atctttttgt gacctgacca caatttaaaa caaaggtctc tgattcatct tccacaatcg
     4381 tggtgatcga cttaagtata taatacccgc caaaaataat aacagcagag gaaataaatc
     4441 ctaaagctaa tgagacatct aacatggaag ttctcctttt ttaacaaaag ttaaacgtga
     4501 atctgtcatg agttctgtac gacgatgttg caaatagtaa tttaaactgc cttgatctaa
     4561 gagtttttcc agaacacccg caatggtttg aatttctaca gccaccgctt gggccctggt
     4621 tttggcaatc tcgacccctt gctcagccgc cgctattctc tgccgcgagt ccgcttccgc
     4681 acgtgaataa attgttttag cctctgtggt ggcgctttga attccattca aagctttctc
     4741 caattctgga ggaggtaaaa tatcaatcaa atcgacggca ttaaattgaa caccatagga
     4801 actaccaatt tgtttttggc aaaaattctc aataagaaag ttaaagtgat tgcgatcacg
     4861 acgaatctcc gcataagaac cgacaaactc ccgcgaatct ccgtcaacct tgctgttgca
     4921 aaaatttgcg atttcatttc gtatcaaaca ggtaaataat tcctttatat gctccatggg
     4981 gtttttgagg ccaaacatat aagcataaag atgtttttca agaggcttga acctaatttt
     5041 cgagtctatc ctcaaaatcg ttccgtccgc cgtcatcgca tgacgtccac catccgggcc
     5101 tgataaatct aaaactcttt ccatggttga aaagcagtgg actttttccc aaggccatat
     5161 ccaatggacc cccggtttaa aggttttcac tcttctatgg tcaataaaat ccagcggatt
     5221 cacatcggat gttgactctt catcttcata cacaactgcg ccaaaacgag tcagcacagc
     5281 cgcatgtcct tcctcaactt taaaaactga attccacgcc caaataact
//
LOCUS       DATBTE010000046        28630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_210030, whole genome shotgun sequence.
ACCESSION   DATBTE010000046 DATBTE010000000
VERSION     DATBTE010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 28630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28630
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210030"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VIG33_01840"
     CDS             complement(<1..550)
                     /locus_tag="VIG33_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269603.1"
                     /translation="MDLDLGLPGLGGERPFCYLIDMNVVYFTLGESISDIEVRAQLLR
                     VPEVLADLRQAQNLIPNMDLLSIMGSQELFKKISKEFQLKLAQLLQEALFKRWKRAEI
                     RYDIILERRKLSDSAVWRKSLKDLLHNSAEFQIYVFGPGFDDLEYEINKLKFKVPPQI
                     FLHEVISEDPLLDWFWPNITQDA"
     gene            651..1583
                     /gene="gspC"
                     /locus_tag="VIG33_01845"
     CDS             651..1583
                     /gene="gspC"
                     /locus_tag="VIG33_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HEY8269604.1"
                     /translation="MAVDNKKLNKKRPAFEKWLSYLLFVFIGFVVADLLILSYRDLML
                     PNQTPPARPKKILNDNLVSRGAFNTLISRNIFSADGVIPDALIPKGQDPNKMNEQAPV
                     PSQLPLNLVGTLVHSNPEKSIAAIEIRGKNQTLSYTPKRNIENLATLLKVERQKIIIR
                     NLNTNTLEFIEMKPFGNQKVSFGATAPVAPTAGGSGEVKNLGDNKFTIKRADLQKYLN
                     DLSSILMQARAVPVRDKVTGEMIGYRLVDFQPNSIFSQLGIQRMDLIKSVNGEPVDTP
                     QKAMELFNMFKNSNNIKMSVDRGGRSETLDYSIQ"
     gene            1621..3855
                     /gene="gspD"
                     /locus_tag="VIG33_01850"
     CDS             1621..3855
                     /gene="gspD"
                     /locus_tag="VIG33_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEY8269605.1"
                     /translation="MKLKKRQRTVLGMASLLTTQIALAQGFPPPPPIPSNEIFTPPPP
                     PPDMDSGTSGSANPGKPGALSKPQQSKFAKAGLEDITNENFPETIESFDFPNVEITDV
                     IKAISELTGKNFIIDPGVRGKITIVAPSKITVAEAYKAFLSALAINGFTVVPSGSFLK
                     IKSARNAQRDSIETYSGSYYPNSDQMITRIIHLKHIQADQVNRDLRILSSKDGEMSVY
                     TATNSLILSDYGSNIDRVMKIINQLDVPGFEDQIEVIQIKYAKAKDLADLVDKIVNKG
                     AKSTGSAPGAFSAGVPRFSRSSGTSAQQGTSFFMAIPEDRTNSIIAMGNKSGIIRLKK
                     LIAQLDFRIRAGDAGGVYVYYVKNGVAKTMAQTLQGVTKDAAPKSGGSSLLSPLGGNP
                     VASQATEIFGGDVKITADESTNALVITASKQDYEMVLNILNKLDIARDQVFVESIIME
                     MSNTDANNWGIGYYKYGDSGYGKAGFVSSGFDLGSALSPIGGTGATLGFGDGKTVTVT
                     DPSTKSQITIPNLIGFINFIKSNTKANILSTPQVLALDNQTAEIEVGDSIPVSVTQTV
                     TATGQTTSANFQEATIKLTIKPFISPASETIRMEIDQKISQPTQVTLPKGLSDNSQPL
                     AKRNIKTTLVIKNEDTAVLGGLMKDKETEKVSKVPLLGDLPIIGWLFKGREVSKEKVN
                     LLVFLTPKIIRNPGDQKAIINRKLDDRLKYIKSQGGKDPFGETVDRVGGKKTSKMTDD
                     NNGE"
     gene            3892..5586
                     /gene="gspE"
                     /locus_tag="VIG33_01855"
     CDS             3892..5586
                     /gene="gspE"
                     /locus_tag="VIG33_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEY8269606.1"
                     /translation="MASMDIPNILQKTTSLTSDQIKSILKNPSVLRPITIGEALSQRE
                     HTSAEDLLSDLCKEMGLEFIKDIPVGDISADLVRDIPINYAKTNSILPYKDELDAVTA
                     LTSNPLNLKAMDDLRVLFGKKVKPLVSTSSRIQDAINRVYEKSTANLSGMDDIDAEDY
                     DLEESSVDLLDAGEDDAPVIKLVNSLLFRAVKEKASDIHVEPYEKDIVVRFRIDGILF
                     DIFKPPKKLQNAITSRIKVMANLNIAEKRLPQDGRIPLKVGGKDIDIRLSTVPTAFGE
                     RAVMRLQDRSNVVLELTQLGFSPETLNKLDNDLLLRTYGILLVTGPTGSGKSTTLYGC
                     LSKLNQPDVNILTVEDPVEQRIHGIGQVQINSKIGLTFASGLRSFLRQDPDIIMVGEI
                     RDLETAELAITSSMTGHLVLSTLHTNDAAGAFPRLFDIGVEPFLIATSILGVIAQRLV
                     RILCPHCKVPYEPSDFEINALGVTRQQVLAGNVCKAVGCNQCNQKGYLGRSVIQELLV
                     VNDDIRSLILRRKDGGSIKKQAVNNGMLTFRDHGITKVLAGITSIDELNANTQLDI"
     gene            5613..6833
                     /gene="gspF"
                     /locus_tag="VIG33_01860"
     CDS             5613..6833
                     /gene="gspF"
                     /locus_tag="VIG33_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEY8269607.1"
                     /translation="MPIFEYKGLSKDGRNVKGIIDAENLRAARTRLKKDRIFVVEIRD
                     KQKSSNARKKKAGGGGGKVNVQELSLMTRQLAVLVKANIPLVDCLTAVSEQVENPVLS
                     EAIADCKNMVNEGSPFFKALAKYPNLFDKIYISMVEAGEMSGSLDVILVRLAEFTEAA
                     AELRSKVSSAMTYPIIMLVVTFGLLSLMFIFVIPKMVTVFESAPGLTLPWYTVALVNL
                     STFMVNWWYLIFGAMVVAYLAFNSWRKSPAGSEQWDAITLTLPIAGPVVRMVAVSRFT
                     RTLATLLTGGVPMLQALDIVRNVVNNHVLALAIEDARSNISEGESIAGPLKKSGQFPP
                     IVIHMVSIGEKTGELENMLSQVSDAYDFQVKNKVDGLTGLMGPVVIVIMGLVIAVIVF
                     SVMLPMFDMTSAFG"
     gene            6865..7347
                     /gene="gspG"
                     /locus_tag="VIG33_01865"
     CDS             6865..7347
                     /gene="gspG"
                     /locus_tag="VIG33_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEY8269608.1"
                     /translation="MKLSYCKFNCKLNYKLNCNKLSFAKINNQVNNQKGMTLIEIMIV
                     IAILGGLMALLAPKFLGQKDKANVGQAKIQMGQIANALSMYYNDCGKFPQSLENLVKQ
                     DANCANWGPEAYLKKDPVDPWGNAYVYELNGSEYSLKCLGKDGREGGSAYGADITYEQ
                     "
     gene            7365..7970
                     /locus_tag="VIG33_01870"
     CDS             7365..7970
                     /locus_tag="VIG33_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEY8269609.1"
                     /translation="MKLSLSRGGFTLIEVMIVIAIIAGLIAVGAPRLLKKDANIKTTA
                     RQLTVLVKEIRNQAKLFNSTYRLVIRIETGQESFWVEKSSGPVLIDKEKLKEELEGKN
                     KDKKPEENAPPPLFQIDKRLSKKEKFLPSNLHFAQVETMNMSQPVTSGLAYIHFFPEG
                     LMEASTIQITDKKNTWTLVFNPLTGQADIVEKAVNLKELSR"
     gene            7967..8506
                     /locus_tag="VIG33_01875"
     CDS             7967..8506
                     /locus_tag="VIG33_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY8269610.1"
                     /translation="MIKNKKGFTEKGFTLLETLLAVVILSMGILLLTQSWSGSFLRIR
                     KTQLNQDIAALLERKMAEIDLEFRGKPLETIPEEKEDDFGSENPKYRWKMTSKELQVP
                     NLSSILMNQEGGANEMLMMVIQQLTDHLSKTIKEVKVSVIYTGGKKPLEYFAVTYFAD
                     YNKPLSIGGAGAPAAGGGQ"
     gene            8506..9330
                     /locus_tag="VIG33_01880"
     CDS             8506..9330
                     /locus_tag="VIG33_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HEY8269611.1"
                     /translation="MGGPFKSQAGFTLIEMMITVAILGALTVLATQSIQQAIKQKVKI
                     QDQIDDVSRMRDALRLLEADINQAYHYRDVEKEITDLINKPAPLPPGAPPPIPTPVPP
                     PNPEEAKRTDPTTHFVGTENTVDFVTMNNARIIRNMKQADFMEVGYALKTCKSLSTEQ
                     GSSQCLWRRTTSWVDEDVTKGGDEVVLLENITEFKLRYIGKGKDDWVSVWRTDNGGDT
                     ATKGKFPTAIEISLTIERGAGAKKKKYSMQIVAQIRFPNNEGASNNANTNGAQTTQ"
     gene            9296..10522
                     /locus_tag="VIG33_01885"
     CDS             9296..10522
                     /locus_tag="VIG33_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HEY8269612.1"
                     /translation="MQTRMGLKLLNNQKGMALILAITSVLMISFIAMEVSYDSLVEYN
                     VNINSLNRLKAYYAARSGIELSLLRIKTFQQAKSQFGKQLGGQTEMLDQIWRFPFAWP
                     VPIPGGLSAVDKDSIKGSLKDSIMDASYVVTIEDEGSKLDIGDLVSPSKALQESTRRR
                     LLEIFQGKLKSDEEFQRKYGNYRFEELINAITDWQSKTRTSVSGGNKRQGYSDYPENY
                     PPNRGFRTIQELRLVPGMTEDFYEILEPSITIYGMKAINPNQASKEVLKSLDPEITDE
                     IASAIIKRRDTPNEGGPFKNKEEFWSFITGKGARLGEKTEQTPLVFDKVVNFRIKSTG
                     EYAGVSREITVIVMDLQNAATAVKGYMDQEKKEAAGGAGAAPGGGAGKPAENQAQGQT
                     PALPKGPPRIVYWGER"
     gene            10542..12215
                     /gene="pilM"
                     /locus_tag="VIG33_01890"
     CDS             10542..12215
                     /gene="pilM"
                     /locus_tag="VIG33_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEY8269613.1"
                     /translation="MRSIGIDIGSSSIKVVEVLITSKGFQVTQCYEHPLGLNPAHDQE
                     IEIIEFLREISAKYDPATVRYCLVLRQDQVSIRNKIFPFNDRMKIAKSLAFELEEEIP
                     FSGDNSIFDAKIVRTIGNAAEVLACAAPKHHVRALLQRCSDANIEPALISTEGTAFAN
                     IFEKWNDFPPSNPAPNAPSFEEETPRERSLHLTLNMGHTRTLVCAFEGNSLVSVRTVL
                     WGAKNIADAVTLKYEIPYLEALKEVQNKAFILTNKQGATFDQITFSETIAKSVRELVR
                     DLQLSILEFKSEFNAIITQIGVTGGASSIKNLGPFLTQQLELPVNKVHPLDLIPNVLF
                     EKTPKTDTLFGMALGIAIEGMKKPRNPAINFLKGEFAKQNNYVKNIWERWGPTLKMAT
                     AVLAIFFVYSMLRESFSMGLMDRAEEALKIQAKNVAKLPEKKATEANIKKYIRENKKR
                     AADLRTLASVASMNSAMEILKKINDATPSKGVVTLDVKKLQVHDNDVSIEGYVTNANE
                     LTVLQQALSNLSVEGKIATGNSALKPAVGKKAFSFNFKVDRGITKNISR"
     gene            12217..12915
                     /locus_tag="VIG33_01895"
     CDS             12217..12915
                     /locus_tag="VIG33_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269614.1"
                     /translation="MMNIDDLKDNLKSQLGQTWSKVEDSSFYNQMKDRFQNLSPAHQK
                     VTLMSLGALLALLIISVPYSYYSQSNENVSTYEEKRSLIRELLKVTRESSEVPDLPAA
                     PPAEELKGRVESLMNSARLLPEQIKGVEVITAETGLIPKSLLEAGVRVSMAKLNLRQI
                     VDLGYNIQSISPSVKMNSVNISANAEDPRYFDVEMKLVSLAVPQPHPEISEAADEGDT
                     KGEAKKKPFKRGNR"
     gene            12915..13883
                     /gene="gspN"
                     /locus_tag="VIG33_01900"
     CDS             12915..13883
                     /gene="gspN"
                     /locus_tag="VIG33_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HEY8269615.1"
                     /translation="MIVLIRKAISTLFNSKLKIFVMLFSTIAFLLILFPMNDLGDLVS
                     SQAARVSGNRLFLQFEELKMSLFPTPGMKFEQIFVETPGIPGVSAQEIRFTPSIGGLI
                     SKKPYGSVDAKGIFKGDVQINVKSGTRTENGVERQKIEINAQKLSLQDLREVARLPVM
                     MKGSLNLESSALVDLTFTEQPDIDVKLNMNQFELPPSNISTPMGPLTLPDLKLSTVEL
                     KGRLVGGKLIIENGQIGKEGDELRGHITGNMEITLANAGGSLSPVLGGYTFNVNLRTK
                     RNFQDRAALFLSFIDAYKTATPDGAEYKFKISAANTMVPPSLGAAR"
     gene            14075..14518
                     /locus_tag="VIG33_01905"
     CDS             14075..14518
                     /locus_tag="VIG33_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEY8269616.1"
                     /translation="MAGVNKVIIVGRLGADPEVKAVGQGSTVTRLNIATSENWTGKDG
                     QKQERTEWHRVVVWGKLAEICGKHLSKGRQVYVEGRLQTRSWEDQQGQKKYTTEIVAN
                     TVQFLGSAGGERSADNGKNSAGGEDFGYQDFGPEPSFNSSDDIPF"
     gene            14614..15015
                     /locus_tag="VIG33_01910"
     CDS             14614..15015
                     /locus_tag="VIG33_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269617.1"
                     /translation="MLKASFSLLSLALLTSCSIYSSAGRKQFEEKAPNSLQAFSLQDC
                     RELSAAETWLKEEFPSSSHELVEMHPDYEVWGKPLENGQVEITVFSKTDVNSLLSSTE
                     SCVYKFESKPIWLTYKKSFLNELSRSLANLD"
     gene            15131..16018
                     /locus_tag="VIG33_01915"
     CDS             15131..16018
                     /locus_tag="VIG33_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269618.1"
                     /translation="MKKCYAIFALFIFILTPSAFAAKVSVVKKNKMLVMLEGDSVSVG
                     SEFFVLNSQGKKVALIKITQVKGHRAVADITKGTAQVGYSLQARGGGSGTAASSSSSS
                     SASENYYNKKLSQKVHNGNSWGVVGGYLMNTMQVGTSSIATSMTGTGFGALGFYDYAL
                     SPSITLRAMGGMETYGVAGSNSSASPGSSCTTNCDLKISYLSLYGYGRWNFLQGNYRS
                     WLGIGSGYLYPMSTPTSSVFTSGQLGANQIFVFSAGTDIRMNAKNYIPISLEYGLFPS
                     TATVKANIIYLRAGFAWNL"
     gene            complement(16037..18253)
                     /locus_tag="VIG33_01920"
     CDS             complement(16037..18253)
                     /locus_tag="VIG33_01920"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY8269619.1"
                     /translation="MSESSSESGLSQRPTKTLEDLLEKIRSYFPQADLKVIEKAYYFS
                     EKAHEGQIRRSGEPYISHPLSVAGILADLHLDLDSIMTGLLHDTIEDTHATLEDIRRE
                     FGEAVAQLVDGVTKISQMKFKNSNEKQGENIRKMIVAMGRDVRVLLVKLCDRLHNMRT
                     MNFMPFEKQERIALETLEIYCPLAGRMGISSLKIELEDLCFRYYRPDMYYQLSQKIKK
                     TETERSRYIEEVKALIGSELEKSNFKFEVFGRSKHLWSIYKKMQNRNIDYEQLYDVLA
                     FRVIVDSVAECYGVLGLVHSLWKPIPGRFKDFIAMPKTNNYQSLHTTVMGPGGERIEI
                     QIRTREMHLVAERGIAAHWKYKERGKVDDESEFQQVNWLKDLVNSHQNVKNPDEFLDS
                     VKTDLFDTEIYVFTPKGDVREFPEGATPLDFAYAVHTELGNKCVGARVNGKMVPLRYQ
                     LQNGDTIEIMTSKTQEPSKDWLKYTVTNKAKQKIRQYVKEEQRRRSLSLGKDLVEKEF
                     RKFGMAAVKHLKGPEYELFLRENGLRDLDELYVKVGYGRLEPRHLVDRVSPTNIAKEA
                     AKTEETTFMEKVVRAAAQKTRRSNSLVSVEGLDDVLVHFAKCCHPIPGDPISGFISRG
                     RGITVHRADCPKGFELDQLRKVDVAWTSAQASEGQERMVRVRVISHDVPGLLKSMSEV
                     FAVQGINIESAQIRTTKDKKAVCNFEVSVRDATQLNNAIYELQKIKGILGVSRIFH"
     gene            complement(18380..18622)
                     /gene="rpoZ"
                     /locus_tag="VIG33_01925"
     CDS             complement(18380..18622)
                     /gene="rpoZ"
                     /locus_tag="VIG33_01925"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY8269620.1"
                     /translation="MARVTVEDCLSKVPNRFALCLMVSKRAKQLLKGSECTVAARSNK
                     YIVSALREVAAGNVGYEDANMDPAVLHAEIERDLNK"
     gene            complement(18652..19209)
                     /gene="gmk"
                     /locus_tag="VIG33_01930"
     CDS             complement(18652..19209)
                     /gene="gmk"
                     /locus_tag="VIG33_01930"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_038448884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY8269621.1"
                     /translation="MKTKMIIVAAPSGAGKSSFVERACREEKRLEDTVTYTTRAMRKG
                     ESQGHPYHFIPEEEFKAKIAENFFVEWAEVHTNLYGTPYDQLENAWIQGKCIIMDVDV
                     QGAETFKAKYPDAVSIFILPPSIDELRRRIEKRDGGIPKDIEIRMANAEKELEHASRF
                     DYQIINDQFEHSYAQFKKIVEELLS"
     gene            complement(19206..20084)
                     /locus_tag="VIG33_01935"
     CDS             complement(19206..20084)
                     /locus_tag="VIG33_01935"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY8269622.1"
                     /translation="MKSMTGYGTSKAQQKDLNVEVSIRSVNGRFLDTRFHLPREFVPF
                     EADLKKLLNEKILRGTVDIFVNRKNKATHEAAEVHVNEALAKKYLAAFQKIAKITKLP
                     SQIHLEILARMPDVIQVEEKQEVSTQEEKMLKFVFSKACGDCVKEREREGRFLQKELE
                     KLVRDLEGQVSIVTGLREEANRHLQERFEQKISHRLKNQEVDPQRITQEIVIQLEKAD
                     INEELVRLSEHIKNYKDLLKSAQAEGKKLDFYTQELLREVNTIGSKSQIAKITQAVVE
                     AKTIIERLREQVQNVE"
     gene            complement(20140..21075)
                     /gene="miaA"
                     /locus_tag="VIG33_01940"
     CDS             complement(20140..21075)
                     /gene="miaA"
                     /locus_tag="VIG33_01940"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEY8269623.1"
                     /translation="MEQSKSVVFIVGTTASGKSQWALDMAKRLSGSVVNCDSVQLYKS
                     LNIGAAKPSPEEMALAPHHLYDYVPEGITMTAGNYSRDFFQCLRQLPEGKPVFVVGGT
                     GFYFQAIEKGMYPVRKTSLEVQQQITDILKTDEGAKELYAEFQRLDPEAAKKIHAQDH
                     YRLGRAMELIRTEGKTLTQIQKEFKGKQTPFPYPLLKVGLRWDREILRQRVVFRTRKM
                     LQAGLVAEVEELLARGFAGWSALQSVGYKETLQFLNEGNSAEWLFENIVQNTMGLAKR
                     QRTWFQRDTEIQWFDGSLGFDSAFVAVQKFLDNPR"
     gene            complement(21075..22874)
                     /gene="mutL"
                     /locus_tag="VIG33_01945"
     CDS             complement(21075..22874)
                     /gene="mutL"
                     /locus_tag="VIG33_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEY8269624.1"
                     /translation="MSIEVLSPEVVDQIAAGEVVERPAHLVKELVENSIDAGATAVTV
                     EFSEGGRYVKVVDNGKGIPPEQLPKALERFATSKISKSEDLWKLHTFGFRGEALASIA
                     AVSKLNLTSRPPGCEKAHQLLSEFGKKTSVDPVGGSEGTVITIQELFANIPARLKFLK
                     SDAAEGTQIKTTLKALALAHAEVEFRVYEKNELIFFWPACQSRKARVEQILEIKPMYE
                     GRAQRENVTAHAVFADPHQVAKTARNIWLFAQNRWIQDRSMQAAVMEAYRNLLMHGEY
                     PVAAVWVEVDPEYIDVNIHPTKSQVKFHSPSLAFRAVQAAVRETLEKAPWTPEAQISD
                     RQNSQAQNLQTRSFNEFDLKESVSTSQTQSSFHHPSFHATQFKKKDFSLQVAAEQAEI
                     RREAMSHVTPQGSYWSSLEVLGQANLTYIICQSRDKVVFVDQHAAHERVVFERLMSAW
                     RGGKVEVQEFLFPLAVDLTPDKVEALQNLFPDIEKLGVFVEVLGPTTVGVRAAPTLIK
                     ESILSSVLDKMAGEALDLGGSFSMEKVVGDICATMSCHSVVRAGQALSIEQMRNLLNE
                     MDEFPLSSFCPHGRPVSVDYPYAKLEKDFGRSV"
     gene            complement(22898..23773)
                     /locus_tag="VIG33_01950"
     CDS             complement(22898..23773)
                     /locus_tag="VIG33_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY8269625.1"
                     /translation="MKAIQCLFFMILFFVGWGASAQSQKAVVLNEGALVYKEADFDAP
                     VLTRFKAGQVYEISLKTKGPFYKVRVKPGVVGWIADTDVRPKNAAKVQNDKGVTVKKP
                     DKKEENDSFPEKKKPQKSFYSSRYRGPMLAYLSYTENTMGDLRTQTLPFYGVKIAGPN
                     TMFDGDLRTDAEFLFHSGAPAYYQTSTGFPASGWTFLTDFIFETASPQSKWHMLTFGF
                     GPMFKYSHYEVSLNALQNAKQATYSLDDMVFGAVFDVGLAFRIADTYALRFNVKYYWE
                     KTKYWGGSLTFGFPF"
     gene            complement(23780..24475)
                     /locus_tag="VIG33_01955"
     CDS             complement(23780..24475)
                     /locus_tag="VIG33_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEY8269626.1"
                     /translation="MKALIIIPTYNEKENIQNIVQAVFAQKLGVDILVVDDSSPDGTA
                     QIVQEMQKNHPQLHLLSRPGKQGLGRAYIAGFRWGMENGFDVLTEMDADFSHRPEDLG
                     PLLNKLKDHDFAVGSRYVDGGRTVNWGVMRKIISRGGGLYSRLILGFPLNDWTGGFNA
                     WKREVLLGIDLSTVVSNGYSFQIELKYKALRKGFKGAESPIVFEDRRVGHSKMSLKIV
                     LEAFYRVWLMRFN"
     gene            complement(24532..25020)
                     /locus_tag="VIG33_01960"
     CDS             complement(24532..25020)
                     /locus_tag="VIG33_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269627.1"
                     /translation="MPILKSKPKFKLKSKNNCYCAFCRTPRRAYKKRNIGFLNILYAA
                     VTSSLMMYCFWGAFDPRVLVIFAMALGITETFIQIRWRLSISCKQCGFDPVLYLKDAD
                     KAAEKVNEHLKQRRLDPQYLLARPLNLPSISPEKAEALHSQRQIKAQSKDKKGVLVSR
                     QA"
     gene            25124..25780
                     /locus_tag="VIG33_01965"
     CDS             25124..25780
                     /locus_tag="VIG33_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269628.1"
                     /translation="MTHKKFLKLASLGLLTQFLSTMVWATGNLGESYQEVTYDELVNE
                     LNTKKSTVESKKKATINEIHLGVGYVHSYTQLNVNRKNTERFQNGLQLSASMNLDSPN
                     LYTEGVFRNFSGSTISQEDLQILQIDGRLGYLNALTAPWKFNLFTGFSGRLIQGNNPV
                     QYYSINEFTPSFTTGLGILAEIHKNIRLGFEVGGRTSILGKNTERNSVDFALHLDTSL
                     "
     gene            25783..26385
                     /locus_tag="VIG33_01970"
     CDS             25783..26385
                     /locus_tag="VIG33_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269629.1"
                     /translation="MENTDALVILFFLPFLLLGLMVSTNFYRELRQYNFELKPNCLLT
                     RKPVVFINGPCSIFYFRKYWNAYPEILTEHGYEVFSLHLPWRGPARFTKMQKFLNQQE
                     INSNKYHFICDEFSSAELEGLFKYSRSVASLTILKNKEELSSQDTYSRSFLNWSYKLH
                     SWSCKSLVLPTAEDLGVQFPTSASWLLEKMQENGEQDFLG"
     gene            26446..27036
                     /locus_tag="VIG33_01975"
     CDS             26446..27036
                     /locus_tag="VIG33_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HEY8269630.1"
                     /translation="MRDVSEPTPPEQRVKNILKYILQFLKHPLHEIAHLPDWSWKTLA
                     WIQVICAMISGFAAGLTKPGFFSILAGLIVTPFIAMLMVLVLTAFLYYYFQVFEKRTV
                     PASKLFALAVLSSIPFFILQIGSSLLPPITLIGFGFSTMLIAVGLTENFQMEKYRAIR
                     LSIVLFTIVLLVWVGNKISVYRLDRAANQQPFTLEQ"
     gene            complement(27050..27499)
                     /gene="dut"
                     /locus_tag="VIG33_01980"
     CDS             complement(27050..27499)
                     /gene="dut"
                     /locus_tag="VIG33_01980"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEY8269631.1"
                     /translation="MKTLIVKIKKLENFKGEIPQYQSSGASGVDVRAQLTTPMTLKPG
                     ERAMIPTGLSFEIPLGFEIQARPRSGFAAKQGITVLNTPGTIDADYRGEVKIIVINLG
                     HESVLIQDQERIAQLVIAPVIQAQFEVVETLSSTERGIGGFGSTGRA"
     gene            complement(27536..>28630)
                     /locus_tag="VIG33_01985"
     CDS             complement(27536..>28630)
                     /locus_tag="VIG33_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY8269632.1"
                     /translation="TKTRSAYQIAKSLEALGGDLNAYTTREYTCYHALVLKDHWEKAL
                     DVLCDLISNMQISTNDFRLEKGVILQEIAMTDDTPEELIYDVLFEKAYAGNPASTPIL
                     GTVESVASMSQKRVTDYYKSRYTGKNIIVTAAGNLDHQAFLNAVGERLAKKKKTKFLA
                     KRERPRWKTARVVSEKSSEQTHMLMGLPTASFQDSQRFEAYIVNTLLGGGMTSKLYQS
                     VREKKGLVYSIHSMLNTFVDSGAMLIYAGTEVKSARKVAELISKELRKLKKSGISSSD
                     VELFKTQVIGSILLGSDDIENRMTSIAVNEMVFGDYRSVDSVIQQIQKVTKASVNEFI
                     QERFDTSKVSGVLMGAGVENLDKWWRDFEF"
BASE COUNT         7970 a         6981 c         6070 g         7609 t
ORIGIN      
        1 tggcatcttg agtgatgttg ggccaaaacc aatccaacaa gggatcctcc gaaatcactt
       61 catgtaagaa gatttgcgga gggaccttga attttaattt attgatttca tattccaggt
      121 catcaaaacc agggccaaaa acataaatct gaaactccgc actgttatga agcaaatctt
      181 taagggactt acgccaaacc gcagagtcac tcaactttcg cctttccaag ataatatcgt
      241 aacgaatttc tgcacgcttc caccgcttaa acaaggcttc ctggagaagc tgcgccaatt
      301 ttaattgaaa ctctttggat attttcttaa agagttcctg ggaacccatg atagaaagca
      361 aatccatatt tggaattaaa ttttgagcct gacgaagatc tgcgagcact tcaggcacgc
      421 gcaatagttg cgcacgaacc tcaatgtcag aaatagactc cccaagagtg aaataaacca
      481 cgttcatatc aataagataa cagaacggac gctcaccacc gagtcctggc aggccaaggt
      541 ctaggtccaa ttttcttcct gccaaggagc ccgcattttt gctaggataa gtttggactt
      601 gaaggattca aatccctgat ttgacaaagg attggtaaag gaattaacca atggccgtag
      661 ataataagaa gttgaataaa aagcgcccag cttttgaaaa atggctgtcg tatctcctct
      721 ttgtctttat cggctttgtc gtggctgatt tgctcatctt aagttatcgc gatttaatgc
      781 tgccaaacca aactcctcca gctcgcccga aaaaaatctt aaacgacaat ttggtttccc
      841 ggggagcctt taacactctt atttcacgta atatcttctc tgcggatggg gttatccccg
      901 atgccttgat tccaaaagga caagatccta acaaaatgaa tgagcaagct cctgtgcctt
      961 cacaacttcc actgaatctt gtcggaactc tggtccattc caatccagaa aagtccatag
     1021 ctgctatcga aatccgcgga aaaaatcaga ctttatctta tactcccaaa agaaatattg
     1081 aaaacctggc aactctgctg aaggttgaac gacaaaaaat aattatccgt aacctcaaca
     1141 ccaatactct tgagtttatc gagatgaaac cttttggaaa tcagaaagtc tcctttggtg
     1201 cgacagcacc cgtcgcgcca actgcgggcg gctcagggga agtgaaaaat ctgggtgata
     1261 acaaattcac cattaaacga gcggacctgc aaaaatatct caacgatctt tcgagcattc
     1321 ttatgcaagc ccgcgcggtc cctgtccgtg ataaagtgac tggcgaaatg atcggatatc
     1381 gtctcgtgga ttttcagccc aacagtattt tctctcaatt aggtattcaa cgtatggact
     1441 tgattaaaag tgtgaatgga gagcccgtgg atacgcctca aaaggcaatg gaactcttca
     1501 atatgtttaa gaactcaaat aacatcaaaa tgagtgtgga tcgcggtggc cgcagtgaaa
     1561 cgcttgatta ctcgattcag tagtttttaa ggttttagtc cgcaaaggaa ggtggatgaa
     1621 atgaagttaa aaaagagaca gaggaccgtg ttgggaatgg cttccctcct gaccactcaa
     1681 atcgcgttgg cccaaggatt tccaccgccg cctcccatcc cttctaatga gatttttact
     1741 ccaccgccac ctccgcctga catggacagc ggaaccagcg gcagcgcgaa ccctggaaaa
     1801 cctggagccc tgagcaagcc tcagcagagc aagtttgcaa aagcgggcct cgaagatatc
     1861 accaatgaaa atttcccaga aactattgaa tccttcgact ttccgaatgt ggaaatcacg
     1921 gacgtgatca aagcgatttc tgaactcacc ggcaagaatt ttattatcga tcccggagtt
     1981 cgcggcaaga tcaccatcgt tgcccccagc aagatcactg ttgcggaagc ttacaaagcc
     2041 ttcttatccg ctctggcgat taacggcttc accgtggtcc ctagcggcag ctttttgaaa
     2101 attaaatccg caaggaatgc tcaacgggat agtattgaaa cttactctgg ctcttattat
     2161 ccaaactctg atcaaatgat cacccgtatt atccacttga agcacattca ggcggatcaa
     2221 gtaaacagag atctccgcat cctttcctct aaagatggag agatgtccgt ctataccgcg
     2281 accaactctc tgattttgtc ggactatggc tccaacattg atcgcgtgat gaaaatcatc
     2341 aatcagttgg atgttccggg ttttgaagat caaattgaag tgattcaaat taaatacgcg
     2401 aaagcgaaag atctggccga tctcgtcgat aaaatcgtga ataaaggcgc gaaaagcaca
     2461 ggctccgccc ccggtgcttt ttcagcaggg gttcctagat tctcccgttc ctcaggcacc
     2521 agtgcgcaac aaggaaccag cttctttatg gctattcctg aagaccgcac caactccatc
     2581 atcgccatgg gaaacaaaag cgggattatt cgccttaaaa agctcattgc ccaactcgac
     2641 tttcgcattc gtgcgggcga tgccggtggc gtttatgttt attatgtaaa aaatggcgtt
     2701 gcgaaaacaa tggcgcaaac tctgcaaggt gtgacgaagg atgcggctcc aaaatccggg
     2761 ggcagcagct tgctcagtcc tttagggggc aatccggtgg cgagccaagc cactgaaatc
     2821 ttcgggggcg atgttaaaat cacagcggat gaaagcacca atgccctggt cattaccgcg
     2881 agtaaacaag actatgaaat ggttttgaat attctcaata aactcgatat tgcccgtgat
     2941 caagtcttcg tagaaagcat tatcatggag atgtccaata ctgatgcgaa caactggggc
     3001 atcggctatt acaaatacgg cgacagcggc tatggcaaag ccggtttcgt cagcagcggt
     3061 tttgatctcg gaagtgccct aagccctatc ggaggaaccg gagcgacact gggctttgga
     3121 gatggcaaaa ccgtgaccgt caccgaccct agcacaaaaa gtcaaatcac cattccaaat
     3181 ttaatcggat ttattaactt tattaagtca aataccaaag ccaacattct ttccactcct
     3241 caggttttag ccttggataa tcaaacagcg gagatcgaag tcggcgatag tattccagtc
     3301 agcgtcacac aaacagtcac tgctacaggt caaacaactt ctgccaattt ccaagaggcc
     3361 accatcaagc tgaccatcaa gccctttatc agcccagcct ctgagacgat tcgtatggag
     3421 atcgatcaaa agatctcgca gccaactcaa gtgactttgc caaaaggctt atcagacaac
     3481 tcgcaacctc tcgcaaaacg taatatcaag acgactttag tgatcaagaa tgaagacact
     3541 gcggtgctgg gcggcttgat gaaggacaaa gaaactgaaa aagtctctaa ggtgcctctt
     3601 ttgggcgatt tgccaattat tggttggttg tttaagggtc gagaggtgtc caaagaaaaa
     3661 gttaatttat tggttttcct gactccgaaa atcatccgca accctggcga ccaaaaagcc
     3721 atcatcaacc gcaaattgga tgaccgcttg aaatatatca agtcccaagg gggcaaagat
     3781 ccattcggag aaacggtgga tcgcgtgggc ggtaaaaaaa catctaaaat gactgacgac
     3841 aataatgggg aataaagtta ataatataaa gcagggggag taaagcactc gatggcttca
     3901 atggatattc cgaacatttt gcaaaaaacc acttcgctta ccagcgatca aatcaagtcc
     3961 atcttgaaga acccctccgt tctgagaccc ataacgattg gcgaagccct ctcgcaacga
     4021 gagcacacct ccgcagagga cctcttgagc gatctctgca aggaaatggg ccttgagttt
     4081 attaaagaca ttccggtggg cgatatctcc gcggatctgg ttcgcgatat ccctattaac
     4141 tacgcaaaaa cgaattccat tctgccctac aaagatgaac tcgatgccgt gaccgccctg
     4201 accagcaatc cgttgaattt gaaagccatg gatgatcttc gcgttctgtt tggcaaaaaa
     4261 gtgaaaccac tggtgtccac cagttcccga attcaagatg ccatcaaccg cgtctatgaa
     4321 aaaagcacgg cgaatctttc gggaatggat gatattgatg ctgaagatta tgaccttgaa
     4381 gaatccagcg tggatctttt ggatgccggt gaagatgatg ctcccgtcat taaactggtg
     4441 aacagtcttt tattccgcgc agtgaaagaa aaagcgtctg atattcacgt ggaaccttac
     4501 gaaaaagaca ttgtcgtccg ctttcgtatt gatggtattt tatttgatat tttcaagccg
     4561 cctaagaaac tgcaaaatgc catcacttcg cggattaagg ttatggcgaa tttaaatatc
     4621 gccgaaaaac gcttgcctca ggacggtcgt attccactga aggttggggg taaagacatc
     4681 gatattcgtc tctcaacggt gcccaccgct ttcggagagc gcgcagtcat gcgtctgcaa
     4741 gatagatcca acgtggttct tgagctcact caattgggtt tctcgccaga gactctgaat
     4801 aaactggaca atgatctctt gcttcgcacc tatggaattt tactggtcac aggtcctaca
     4861 ggttcgggca aatccacgac gctgtatggc tgtctgtcca agctcaatca accggatgtg
     4921 aatattctca cagtcgagga tccggtggag caacgaattc acggaatcgg ccaggtacag
     4981 atcaactcga aaattggact gaccttcgct tcaggtctcc gctccttcct tcgtcaagat
     5041 cccgacatca tcatggtggg ggagatccgg gacttggaaa ccgcagaact ggccatcacc
     5101 tcttcgatga caggtcactt ggttttgtcc actttgcata ccaatgatgc cgccggcgcc
     5161 tttccccgtc tcttcgatat cggagtggag ccatttttga tcgcgacttc catattgggt
     5221 gtgatcgcgc aacgtctggt gcgcattctc tgccctcatt gtaaagtgcc ctacgagcct
     5281 tcagattttg aaatcaacgc tttaggtgtg actcgtcagc aagtcttggc gggaaatgtc
     5341 tgcaaagccg tgggttgcaa tcaatgcaat caaaaaggtt acctgggacg aagtgtgatc
     5401 caagaactgc tggtggtgaa tgatgatatt cgctctctga ttttacgacg taaagacggg
     5461 ggtagtatta aaaaacaagc cgtgaataat ggaatgctca ccttccgtga ccatgggatc
     5521 accaaagttc ttgcgggaat cacctccatt gatgaattaa atgcgaatac ccaactggat
     5581 atttagcgaa cgattttata ggagattttt aagtgcccat ttttgagtac aaaggccttt
     5641 ccaaagacgg aagaaatgtc aaaggaatca ttgatgcaga aaatctgcgt gcggcccgta
     5701 ctcgccttaa aaaagatcgc atcttcgtag tggaaattcg cgataaacag aagagttcca
     5761 acgccagaaa aaagaaagcc gggggcgggg gcggaaaagt caacgttcaa gaactctctt
     5821 tgatgacccg tcagttggcg gtgctggtga aagccaatat tcctcttgtc gattgcttga
     5881 ccgcggtttc agagcaggtg gaaaaccccg tgctttccga ggccattgcc gactgtaaaa
     5941 acatggtcaa cgaaggaagt ccttttttta aagccctggc aaaatatcca aacttatttg
     6001 ataagattta tatttccatg gttgaggccg gagaaatgtc cggcagcttg gatgtcatcc
     6061 tagtccgcct ggcggagttc accgaggccg ccgccgagct gcgctccaaa gtgagcagtg
     6121 ccatgactta tcctatcatc atgttggtgg tcaccttcgg tttgttatca ctcatgttta
     6181 tttttgtaat ccctaaaatg gtgaccgtgt ttgaatccgc tccgggcctc acccttcctt
     6241 ggtacactgt cgcccttgta aatctcagta cttttatggt gaactggtgg tatcttattt
     6301 tcggagccat ggtcgttgcc tatttggcat ttaacagttg gagaaaatct ccggcaggct
     6361 ctgagcaatg ggatgcgatc actttaaccc ttcctatcgc cggccccgtt gtcagaatgg
     6421 ttgcggtttc tcgttttacc aggacccttg caactttgct tacgggagga gtccccatgc
     6481 ttcaggcttt ggatatcgtt cgaaacgtgg tgaataacca tgttttggct ctcgcgattg
     6541 aagacgcccg cagcaatatc agcgaagggg aatccatcgc aggtcccctc aagaaatcgg
     6601 gacaattccc cccgatcgtg attcacatgg tcagcatcgg cgaaaagacc ggtgagctgg
     6661 aaaatatgct gagtcaagtt tctgatgcct atgactttca ggtaaaaaac aaagttgacg
     6721 gtttaacggg acttatgggc cctgtagtga ttgttattat gggccttgtc attgcagtca
     6781 tcgtattctc tgtgatgtta ccgatgtttg acatgacgag tgcctttggc taaggtagcc
     6841 tctcaatatc cggaggcaaa ttccatgaag ctgagctact gtaaattcaa ctgtaaattg
     6901 aattataaat tgaactgtaa taaattgagc tttgcaaaaa ttaacaacca agttaacaat
     6961 caaaaaggca tgacactcat tgagatcatg attgtgatcg caattctggg cggcttgatg
     7021 gctcttttgg caccgaaatt tttgggtcag aaagataaag ccaatgtggg ccaagctaaa
     7081 attcaaatgg gccaaatcgc caatgccctc agcatgtact acaatgattg cggcaagttc
     7141 ccccaatccc ttgagaactt ggtgaaacag gatgccaatt gcgccaattg gggacctgaa
     7201 gcttacttga aaaaagatcc cgtagatcct tggggtaatg cctatgttta tgaactcaat
     7261 ggttctgagt actcactcaa atgtcttggc aaggatggcc gcgaaggtgg ctcggcttac
     7321 ggcgctgata ttacctacga gcaatagagc cttcaaggag ctgcatgaag ttatcacttt
     7381 ctaggggggg cttcaccctc attgaagtca tgattgtgat tgctattatc gcgggcctca
     7441 ttgccgtcgg ggccccccgc ctccttaaaa aagatgctaa cataaaaacc acggctcggc
     7501 agctcacagt tctcgtcaaa gaaatccgca atcaagctaa actttttaat tccacctacc
     7561 gtctggtcat tcgcatagag acgggccaag agtctttttg ggtggaaaag tccagcggcc
     7621 ccgtcttgat cgataaagag aaattaaaag aggaactaga gggaaaaaac aaggataaaa
     7681 aaccggagga aaacgccccc cctccccttt ttcaaataga taaaagactc tctaaaaaag
     7741 aaaaattttt accttcaaat ctccacttcg ctcaggttga aaccatgaat atgtctcaac
     7801 ctgtcacgag cgggcttgcc tatattcatt tctttccaga gggattaatg gaagcttcca
     7861 cgatccaaat cactgataag aaaaacactt ggacacttgt ctttaatccc ctaacaggcc
     7921 aagctgatat tgttgaaaaa gccgtaaact taaaggaatt aagtcggtga tcaaaaacaa
     7981 aaaaggtttt acagaaaaag gttttacctt attagagaca ctgcttgccg tagtgattct
     8041 ctccatgggc attttactcc tcactcaatc ttggagcggc agctttttgc gcatccgcaa
     8101 gacccaactc aatcaggaca tcgccgcctt actggaacga aagatggccg agattgatct
     8161 tgaatttcgc gggaaacctc tggaaaccat tcctgaagag aaagaagatg attttggctc
     8221 cgaaaatcca aaatatcgtt ggaaaatgac ttcgaaggaa ctgcaagtcc ccaacctttc
     8281 ctccatcctg atgaatcaag agggcggcgc caatgagatg ctcatgatgg tgatccaaca
     8341 actcactgat catctttcga aaaccatcaa agaagttaaa gtttcagtta tctacaccgg
     8401 tggaaaaaag cctctcgaat acttcgcggt cacttacttc gcagattaca acaaacctct
     8461 ttctattggt ggagctggcg ctcctgcggc tggcggaggc caataatggg cggccccttt
     8521 aaatcccaag ctggcttcac cctgattgag atgatgatca cggtggccat actcggggct
     8581 ctgacggttt tagccactca aagcattcaa caagccatta aacaaaaagt gaaaattcaa
     8641 gaccaaattg atgacgtctc gcggatgcgc gatgctttgc gtttgctgga agccgatatc
     8701 aatcaagctt atcactatcg agatgtggaa aaagaaatca ctgatttaat aaataaacct
     8761 gctcctcttc ctcctggcgc tcctcctccc atcccgaccc ctgtccctcc ccccaatcct
     8821 gaagaggcga aacgcacgga cccaaccact cactttgtcg gaactgaaaa caccgtggat
     8881 ttcgtcacca tgaacaatgc ccggatcatt cgcaatatga agcaagcgga ctttatggaa
     8941 gtgggctatg ctttgaagac ttgcaaaagt ctcagcactg aacaaggaag ttctcaatgt
     9001 ctgtggcggc gaaccaccag ttgggtggat gaggatgtca ccaaaggcgg cgatgaagtc
     9061 gttttactgg agaatatcac cgagtttaaa cttcgctata tcggcaaagg caaagatgat
     9121 tgggtgagcg tttggcgtac agacaatggt ggcgacacgg ccaccaaagg aaaattcccg
     9181 acagctattg agatttctct gaccattgag cgcggggcag gagcaaaaaa gaaaaagtat
     9241 tccatgcaaa ttgtcgcgca aattagattt cccaataatg aaggggcaag caataatgca
     9301 aacacgaatg gggctcaaac tactcaataa ccaaaaagga atggcgctaa tcttggccat
     9361 cacttctgta ctcatgatct cttttattgc gatggaagtc tcctatgact ccttagtgga
     9421 gtacaacgtc aatataaatt ccttgaatcg cctgaaagct tactatgccg cacgctctgg
     9481 gattgaactc agccttttac gtattaaaac cttccaacaa gccaagtctc aatttggcaa
     9541 acaactcgga ggccaaacgg aaatgcttga tcagatttgg agatttcctt tcgcctggcc
     9601 ggtccctatt cctggaggtc tcagtgccgt ggataaggac agcatcaaag gcagcttgaa
     9661 agactccatc atggatgcct cctacgtggt caccattgaa gacgaaggtt caaaactcga
     9721 cattggtgat ttagtttccc cctcgaaagc tttgcaagaa tccactcgcc gccgactgct
     9781 tgaaattttc cagggcaaac taaaaagcga tgaggagttt cagcgcaaat atgggaacta
     9841 tcggttcgaa gaactgatca atgccataac ggattggcaa tccaagacac gcacctctgt
     9901 cagcggaggc aacaaacgcc aaggttatag cgactatccg gaaaactatc ctcctaaccg
     9961 tggatttcgc accattcaag aattacgtct ggtgccagga atgacggaag acttttatga
    10021 gattctcgaa cccagcatta caatttatgg aatgaaagcc atcaatccca accaagcaag
    10081 taaagaggtg ctcaaatccc tggaccccga aatcacggat gaaattgcca gcgccattat
    10141 taagcgccga gacaccccca atgaaggagg accttttaaa aataaagagg agttctggag
    10201 cttcatcacc ggcaaaggtg cgcgtcttgg cgagaaaacg gagcaaaccc ctctggtttt
    10261 tgacaaagtc gtgaatttca gaattaaaag taccggcgag tatgcaggcg tctcacgcga
    10321 gatcactgtg atcgtcatgg atttacaaaa cgcggcgacc gccgtcaaag gctacatgga
    10381 ccaagagaaa aaagaggctg ctggcggcgc aggcgcggcc cctggtggtg gcgctggaaa
    10441 acctgccgaa aaccaggccc aaggtcagac cccagccctt cccaaaggtc ctcctaggat
    10501 tgtatactgg ggcgagcgct aaccgctaaa aaaggccata catgagatct attggaatcg
    10561 acattggaag tagcagcatt aaagttgtcg aagtgctgat tacaagtaaa ggttttcaag
    10621 ttactcagtg ttatgaacat ccactggggc tcaatcctgc ccatgatcaa gaaattgaaa
    10681 ttattgaatt tctaagagag atttctgcaa agtatgatcc tgcgaccgtt cgttattgtc
    10741 ttgttctccg tcaagatcag gtcagcattc gcaataaaat tttccccttt aacgaccgca
    10801 tgaagattgc taaaagtttg gctttcgaac tggaggaaga aattcctttt tcaggcgaca
    10861 actctatctt tgacgcaaaa attgttcgca ccatcggcaa cgcggccgaa gttctggcct
    10921 gtgcagcgcc taaacaccat gtccgcgctt tgctgcaaag atgttctgac gccaatatcg
    10981 agcccgctct gatatccacg gaaggcaccg cctttgccaa tatttttgaa aaatggaatg
    11041 attttccccc gtccaatccc gcccccaatg ccccttcttt cgaggaagaa acccctcgcg
    11101 aacgaagcct gcatttaaca ctcaatatgg ggcatactcg cactctggtt tgtgcttttg
    11161 aaggcaattc cttggtgagc gtaaggactg tcctgtgggg cgcgaaaaac attgcggacg
    11221 cggtgaccct gaagtatgaa atcccttatc ttgaagctct taaggaagtc caaaacaagg
    11281 cctttatttt aaccaataaa cagggcgcca ctttcgatca aatcacattt tctgaaacta
    11341 tcgccaaatc agtgcgtgaa ctcgtccgtg atttgcaact ttcaattctt gaatttaaaa
    11401 gcgaattcaa cgctatcatc acgcaaattg gtgtgaccgg tggcgcttcc agtattaaaa
    11461 acttaggccc ctttttaact cagcaactgg aactcccagt caataaagtt catccattgg
    11521 atttgattcc gaatgttctt tttgaaaaaa caccgaaaac agacactctc tttggcatgg
    11581 ctttaggaat tgccattgag ggcatgaaaa aacctcgaaa tcccgccatc aacttcctta
    11641 aaggtgaatt tgccaaacaa aataattacg taaaaaatat ttgggaaaga tggggaccaa
    11701 cccttaagat ggcgacggcg gttctggcga ttttctttgt ctactccatg ttgcgggaaa
    11761 gcttttccat gggcctcatg gaccgcgcgg aagaagcttt aaaaatccaa gccaaaaatg
    11821 tcgctaaatt gcctgagaaa aaagccaccg aagcgaatat caaaaaatat attcgcgaga
    11881 ataaaaaacg tgccgctgac ttgagaactc tagcgagcgt ggcctctatg aactcagcca
    11941 tggagatcct gaaaaaaatc aatgatgcga ccccttcaaa gggggtagtg actttagatg
    12001 taaaaaaact ccaagtccat gacaatgacg tctccatcga aggctacgtc accaatgcca
    12061 atgaactcac cgtattgcaa caagccctca gcaatttgtc cgtggaaggc aagatcgcta
    12121 caggaaactc agcactgaag cctgccgttg gaaaaaaagc attttcattc aattttaaag
    12181 tcgaccgcgg catcacaaaa aatatcagta ggtgagatga tgaacattga cgatttgaag
    12241 gacaatttaa aatctcaact aggacaaact tggtcaaagg tcgaagactc ctctttctat
    12301 aaccaaatga aagaccgttt tcaaaatctc agccccgctc atcagaaggt gacattgatg
    12361 tcattgggag ctttactggc tctgttgatc atttccgttc cctattctta ctattcccaa
    12421 tcaaatgaga acgtttccac ttatgaagaa aaacgcagtc ttatccggga actgttgaag
    12481 gtgactcggg aatcttccga agttcccgat cttccagccg ctccccctgc tgaagagtta
    12541 aagggcaggg ttgaaagtct catgaactcg gcccgccttt tacctgagca aataaaaggg
    12601 gtggaagtca ttacggctga aacaggactg attccaaaaa gtttgcttga ggctggcgtg
    12661 agagtttcca tggctaagct gaatcttcgt cagattgtgg acttgggtta caatatccaa
    12721 agtatcagtc ccagcgttaa aatgaattca gtgaatattt cagcaaatgc agaagatccc
    12781 cgctattttg atgtagagat gaaattggtt tccctggcgg tccctcagcc tcacccagaa
    12841 atttccgaag cggctgacga gggcgacact aaaggtgaag ccaagaaaaa accgtttaaa
    12901 agaggaaatc gctgatgata gttcttatcc gcaaagccat tagcactctc tttaattcaa
    12961 agttaaaaat tttcgtcatg ctattttcca caatagcatt tctgttgatt ttatttccca
    13021 tgaacgatct tggggattta gtctcttcac aagcagctcg cgtgagtggc aacaggttat
    13081 ttcttcaatt tgaagaactg aaaatgtcac tcttccctac tccgggaatg aaatttgaac
    13141 agatcttcgt tgaaacccct ggtattccag gagtttccgc tcaggagatc cgcttcaccc
    13201 cttccatcgg tgggttgatt tcaaaaaaac cctatgggag cgtggatgcc aaaggaatct
    13261 tcaaaggcga tgttcagatc aacgttaaat ccggcactcg cactgaaaat ggagtggaaa
    13321 gacaaaaaat cgagatcaat gcacaaaagc taagccttca ggacctgcgc gaagtggctc
    13381 ggcttccagt gatgatgaag ggaagcttaa atctcgaaag cagcgctctg gtcgatctca
    13441 ccttcaccga acaaccagat atagatgtga agctgaatat gaaccaattt gaattgcccc
    13501 cctccaacat ctcgactccc atggggccgt tgactttgcc agatttaaaa ttatcaaccg
    13561 tggagcttaa aggccgcctt gtcggcggaa aactcattat tgaaaacggc caaataggta
    13621 aagaaggcga tgaactccgc ggccatatta cggggaatat ggaaatcact ttagccaacg
    13681 ctggaggaag cctctctcct gttctaggcg gctatacttt caacgtgaac ttaaggacga
    13741 aacgcaactt tcaagaccgt gcggccctgt tcttgtcatt tattgacgcc tataaaacgg
    13801 ccaccccgga cggcgctgaa tataagttca agatctccgc tgccaacacc atggttccac
    13861 cctcactagg tgccgcgcgt taatccgaga aatcccccct ctgaatccga aatctgaatg
    13921 ccacttcatg ccaaatgaaa ctcgttgaat acaagcctgg tccttgattt gcttattata
    13981 aaagcaaaca aagacaaaga gagaagcttt ttgaagtctt tattgacaag cttctcaata
    14041 attacaaaaa ccttatagtc tcattggagg gattatggct ggtgtaaata aagttattat
    14101 cgtaggtcgt ttaggcgctg accctgaagt taaagccgtt ggtcaaggca gtacagtcac
    14161 ccgtttaaac atcgctacaa gcgaaaactg gacagggaaa gatggtcaaa agcaagaacg
    14221 cactgaatgg catcgagttg tcgtttgggg caaacttgct gaaatctgtg gaaagcactt
    14281 aagcaaagga cgtcaagtct acgtagaagg tcgcttgcaa actcgctctt gggaagacca
    14341 acaaggccag aagaaataca ccactgaaat tgttgccaat actgtgcaat ttttaggttc
    14401 cgcgggtggc gaaagatctg ctgacaacgg taaaaattcc gctggcggtg aagatttcgg
    14461 atatcaagat tttggtcctg agccaagctt caatagttct gacgatattc cattctagga
    14521 tattcgaagg ttttaatatt tcttaaagaa aagactcatt tgatgaaaat gggtcttttc
    14581 tttttctaaa gcttcggcgt agaattttat tctatgttga aggcttcatt ttctcttctt
    14641 agtcttgcac ttttgacctc ctgctcgatt tatagttctg cgggccgtaa acaatttgaa
    14701 gaaaaagccc ccaattcact tcaagctttc tccctgcaag actgccgtga gctcagtgct
    14761 gcggaaacct ggcttaaaga agaatttcct agcagcagcc atgagttggt ggaaatgcac
    14821 ccagattatg aagtctgggg aaaaccgctg gaaaatggcc aggtggaaat tacggtcttc
    14881 tccaaaaccg atgtgaacag cctcctaagt tccacagagt cttgcgtcta taaatttgaa
    14941 tctaagccca tttggctcac ctataaaaaa agttttctca atgaactctc tcgcagtcta
    15001 gctaatctcg attaatccaa tcccccttcc tttcgccccc tctcgaccga agtctgcctt
    15061 ggattgccag tcggtgattt ctgaataaaa ctataaatag aagtcattct ctaaactagg
    15121 agctgttggc gtgaagaaat gctatgctat tttcgctctt ttcattttta ttctgacccc
    15181 ttccgccttt gccgccaaag tttcagtggt aaaaaaaaat aaaatgttgg tcatgctcga
    15241 aggcgacagt gtttctgtgg ggagtgaatt cttcgtgctg aactctcaag gaaaaaaggt
    15301 ggccctcata aaaatcaccc aggtaaaggg ccaccgcgca gtggcagaca taactaaagg
    15361 aactgctcaa gttggataca gcctccaagc tcgcgggggc ggcagcggga ctgctgctag
    15421 ctcttcttca tcgtcctctg ccagtgaaaa ctactataac aaaaagctaa gccaaaaagt
    15481 gcacaatgga aactcctggg gagtggtggg cggctattta atgaatacca tgcaagtagg
    15541 aacttcaagt attgccacca gcatgaccgg tactggcttc ggggctctgg gcttctatga
    15601 ctatgctctt tcccccagca tcacccttcg cgccatgggg ggaatggaaa cctatggagt
    15661 tgcaggcagc aatagttctg catctccagg cagctcttgc accacaaatt gtgacttaaa
    15721 gatctcttac ttgtctttgt atggatatgg acgctggaac tttcttcagg gcaattacag
    15781 atcttggctt ggcatcggct ccggctattt gtatccgatg tcgaccccga caagttcggt
    15841 atttacttca ggacaattgg gagcaaatca aattttcgtg ttctccgccg gaaccgacat
    15901 ccgtatgaat gccaaaaatt atattcctat ttctttagag tatggcctat tcccttctac
    15961 cgcgaccgtg aaggcgaata ttatttatct tcgcgccggc tttgcttgga atctgtaaaa
    16021 aggactcact ttttacttag tgaaagatac ggctgacgcc taagatgcct ttgatttttt
    16081 gcaactcata gatggcatta ttcaactgcg ttgcatctct tacgctgact tcaaaattgc
    16141 acacggcttt tttatctttg gtcgtgcgaa tttgcgctga ttcaatatta attccttgga
    16201 ccgcaaaaac ctcagacatg gactttaata atccaggcac atcatggctg attacgcgaa
    16261 cccgcaccat gcgctcctgc ccctcagagg cttgagcact ggtccaagcc acatccacct
    16321 tacgcagttg atccagttca aaacctttcg gacaatccgc acgatgaacc gtaatgcctc
    16381 ggcctcgtga aataaaacca ctgataggat ctccaggaat cggatggcag cactttgcaa
    16441 agtgaacaag cacatcatcg agtccctcta cgctcacaag agaattacta cgccgtgttt
    16501 tctgagccgc agccctcacc actttttcca tgaaagtggt ttcttctgtt tttgcggctt
    16561 ctttggctat attcgtcgga gaaacccgat ccaccaaatg acggggctcc aggcgcccat
    16621 aaccgacttt cacgtaaagc tcatcaagat ccctcaaacc attctctctt aagaagagtt
    16681 catactcagg gccttttaaa tgcttcaccg ccgccattcc aaatttgcgg aattctttct
    16741 cgaccaaatc ttttcccaaa ctcaaagacc tgcgtcgttg ttcttccttt acgtactgac
    16801 gaattttttg tttggcctta ttagtgaccg tgtatttaag ccaatcttta gaaggctcct
    16861 gagttttcga ggtcatgatt tcgatggtgt caccattttg cagttggtac cgcagaggca
    16921 ccatcttgcc gttcactcgg gctccgacac acttattccc caattccgta tggaccgcat
    16981 atgcaaagtc caaaggcgtt gccccttcgg gaaattcgcg aacgtccccc ttcggagtaa
    17041 atacataaat ttccgtgtca aagagatcgg ttttgacact atccaagaat tcatcaggat
    17101 ttttcacgtt ttgatgggag ttcaccagat ctttgagcca attgacctgt tgaaattcac
    17161 tttcatcatc cactttgccg cgctctttat acttccagtg agccgcaatc ccgcgctccg
    17221 ccaccaaatg catttcgcga gtacgaattt gaatctcgat acgctccccg ccgggtccca
    17281 tcaccgtggt atgcaaagac tgatagttat tggtctttgg catcgcaata aaatctttaa
    17341 aacgccctgg aataggcttc cacagagagt gcaccaatcc caaaacccca tagcactcag
    17401 cgacagagtc aacgattacg cgaaaggcaa ggacgtcata gagctgctcg taatcaatat
    17461 tacgattttg catttttttg taaatggacc agagatgctt ggagcgaccg aaaacctcaa
    17521 acttgaaatt ggatttctcc agctcactgc caataagagc ttttacctcc tcgatgtaac
    17581 ggctgcgctc cgtctcggtc ttttttatct tctggctgag ctggtagtac atatcggggc
    17641 gataataacg gaagcaaaga tcttcgagtt caattttaag ggaactaata cccatgcgac
    17701 ctgcgagagg acaataaatc tccagggtct ccagagctat gcgttcttgc ttttcaaagg
    17761 gcatgaaatt catggtgcgc atgttatgca gacgatcgca gagcttcacc agaagcaccc
    17821 gaacgtcgcg ccccatggcc acgatcattt tgcggatatt ctctccttgt ttctcattgc
    17881 tattcttgaa tttcatttgg gagattttag tcaccccatc cacaagctga gctaccgctt
    17941 ctccaaattc gcgacggatg tcttctaggg tggcatgcgt atcctcgatg gtatcatgaa
    18001 ggagccctgt cataatcgaa tctaagtcga gatgcagatc cgctaaaatc cccgccaccg
    18061 aaaggggatg ggaaatatag ggctcgcccg aacggcgaat ttgcccttcg tgggctttct
    18121 cagaaaaata ataagctttt tcaataacct taagatcggc ttgaggaaaa taagatctaa
    18181 ttttctctaa aagatcctct aaggtttttg tgggcctctg ggagagaccg ctttccgatg
    18241 aactctctga cataccatta aataatagcg cagatgctat gtggcaccaa gaaagaaact
    18301 catttcaggc ttcgttctcg aggtgaattc tatgcagatt tgttcctatt ttcgggtatt
    18361 taggacttta gactaagtcc tacttgttaa gatctctctc gatctcagcg tgtagaactg
    18421 caggatccat attagcgtct tcataaccta cattgcctgc cgcaacttca cgaagagcac
    18481 tgacaatgta cttattgctt cgagcggcca ctgtgcattc agagcctttc aacaattgct
    18541 tcgctctttt tgaaaccatc aaacacaatg caaaacgatt gggaactttg ctcaaacaat
    18601 cttctaccgt aacacgtgcc attcttgcct ccgaaaggag tcaaagtacc tttaagacag
    18661 taattcttca acgattttct taaactgtgc ataagagtgc tcaaattgat catttatgat
    18721 ctgataatca aagcgagaag catgctctag ctccttctcg gcgttagcca tgcggatctc
    18781 aatatcttta ggaataccgc catctctttt ttcaattcga cgacgcaatt cgtcaatgct
    18841 tggcggaaga atgaagattg aaactgcatc gggatatttc gctttgaaag tctcggcccc
    18901 ctggacatcc acatccataa tgatgcattt cccctgaatc caagcatttt cgagctgatc
    18961 ataaggggtt ccataaagat tggtatgaac ctcagcccac tctacaaaaa aattctcggc
    19021 aatcttggct ttgaattcct cttcaggaat gaaatgataa ggatgtccct gactttcccc
    19081 tttacgcatg gctcttgtag tataggtcac cgtgtcttcg aggcgtttct cctcgcgaca
    19141 tgctctttct acaaaactgc tttttccagc cccactgggt gcggcgacaa tgatcatttt
    19201 cgttttcatt cgacgttttg aacctgttct ctaagtcttt caatgattgt ttttgcttct
    19261 accacagcct gagtgatctt ggctatctga gacttcgaac cgatcgtatt cacttctcgc
    19321 aacaattctt gagtataaaa atcaagtttt ttgccctctg cctgggcact tttcaataaa
    19381 tctttatagt ttttaatatg ctccgaaagt cgcacgagct cttcattgat atcggctttt
    19441 tccagttgaa tcacaatctc ttgagtgatt cgctgaggat ccacttcctg gttttttaaa
    19501 cgatgactga ttttttgttc aaagcgctcc tgcaagtggc gattggcctc ttcacgcaag
    19561 cccgtcacga tactgacctg gccttcaaga tcccgcacaa gcttttccaa ttccttttgc
    19621 aaaaacctac cttcacgctc gcgctctttg acacagtccc cgcaggcttt cgaaaaaacg
    19681 aacttcagca ttttttcttc ttgagtcgaa acttcttgtt tttcttcgac ttgaatgaca
    19741 tccggcatac gtgccaaaat ttcgagatga atttgagagg gaagcttagt gatttttgca
    19801 attttttgaa aagccgccaa gtatttcttc gcaagcgctt cattcacatg gacttccgcc
    19861 gcttcatgag tcgctttgtt cttacgattt acaaagatat ctacagtgcc gcggagaatt
    19921 ttttcattga gaagtttttt aagatccgct tcaaagggga caaactcacg aggaagatga
    19981 aatcttgtat ccaagaaacg cccgttcact gaacgaatgc tcacttcaac attgagatct
    20041 ttttgctgcg cctttgaggt gccatatcca gtcatgctct tcatataatc tccagcgtaa
    20101 aacaataaca gattctctgt gccctgggaa gcaaagcctt caccgaggat tgtcgagaaa
    20161 cttttgaacc gctacaaaag ccgaatcaaa acctagggag ccgtcaaacc actgtatttc
    20221 tgtatcacgc tgaaaccagg ttctttgcct tttcgccagg cccatggtgt tttgcacgat
    20281 attttcaaag agccactcag ccgaatttcc ttcgtttaaa aactgaagcg tctctttata
    20341 gccaacgctc tgtaaagccg accagcccgc aaaacctcgg gccaacaatt cttccacttc
    20401 agccactagt ccggcctgca acattttccg ggtgcgaaaa accactcttt gtcttaaaat
    20461 ttcgcgatcc caacgaagcc ccaccttcag cagaggatag ggaaaaggag tttgctttcc
    20521 cttgaactct ttttgaatct gagtcagcgt ctttccttca gtgcgaatca actccatggc
    20581 acgacctaat cgataatgat cttgagcatg aatttttttt gcggcttccg gatccagacg
    20641 ctgaaactcg gcatacaatt cttttgcccc ttcgtccgtt ttcaaaatat ctgtaatttg
    20701 ctgctgaact tccagagatg tttttcggac aggatacatg cctttttcta tcgcttgaaa
    20761 ataaaaacca gtcccaccca ccacgaaaac aggttttcct tctggcaact gtcttaggca
    20821 ctgaaaaaaa tcacgactgt agttccctgc ggtcatagta atcccttcgg gaacatagtc
    20881 gtagaggtga tgaggagcca aggccatttc ttcaggcgaa ggtttcgcgg caccgatatt
    20941 cagagattta taaagctgta cactgtcgca attgaccacg gagcctgaaa ggcgttttgc
    21001 catatccaag gcccattggc tctttcctga ggccgtcgtg ccaacaataa aaacaacgga
    21061 cttggactgc tccattaaac gctacgtccg aaatcttttt caagcttcgc ataaggatag
    21121 tcgacagaga cgggacgtcc atgggggcaa aaactcgaga gtggaaactc atccatctca
    21181 ttgagcagat tcctcatctg ctcaatgctt aacgcctgtc ccgcacgcac gacggaatga
    21241 caagacatcg ttgcgcaaat atcgcccaca accttttcca tggaaaaact cccaccgaga
    21301 tcgagagctt cacccgccat tttatctagg accgagctaa ggatagattc ttttatcaag
    21361 gtcggcgcgg ctctcactcc cacagttgtg gggcctaaga cctcaacaaa cacccccagc
    21421 ttctcaatat cgggaaagag attttgtaaa gcttccactt tatccggtgt caaatccacc
    21481 gccagaggaa aaagaaactc ttgaacctcg accttcccgc ctctccacgc actcatgagc
    21541 cgctcaaaaa ctactcgttc atgagctgca tgctggtcca cgaagaccac cttgtcacga
    21601 ctttgacaga tgatgtaggt gagatttgcc tgacctaaaa cttctaaaga agaccagtaa
    21661 ctcccttgag gcgtgacgtg actcatagct tcgcgacgga tttccgcctg ctccgcggcc
    21721 acttgcaagg agaaatcttt cttcttaaat tgagtggcgt gaaaggaagg atgatgaaac
    21781 gacgattgag tctgagaagt actcaccgat tcctttaaat caaactcatt gaagcttcga
    21841 gtttgtaaat tttgtgcctg tgaattttgt ctatctgaaa tttgtgcctc tggagtccat
    21901 ggagcctttt ccaaagtctc ccgcaccgct gcctgaacag ctctgaaagc cagtgaaggg
    21961 ctatgaaatt tcacctggga ttttgtcggg tgaatattga catcgatata ttcaggatcc
    22021 acttcgaccc aaacggccgc caccggatat tctccatgca tgagaagatt tctataggct
    22081 tccatgaccg ccgcctgcat gctgcgatct tgtatccagc gattctgcgc aaaaagccaa
    22141 atattccttg cggttttagc cacctgatga ggatccgcga agaccgcgtg agcagtcacg
    22201 ttttcgcgct gagcacggcc ttcatacatt ggtttgattt ctaaaatttg ctccacccga
    22261 gctttgcgcg attggcaagc gggccaaaag aatatcaatt catttttttc ataaactcgg
    22321 aactccactt ccgcgtgagc caaagccaga gccttcaaag tggtttttat ttgtgttccc
    22381 tcagcggcat cggactttaa aaactttaac cgagccggga tattggcaaa gagttcttga
    22441 atggtgatca cagtcccttc agaaccgccc actggatcca ccgaagtttt ttttccaaat
    22501 tcacttaaaa gctgatgggc cttctcgcag cctggaggac gtgaggtcag attcagctta
    22561 ctgacagcgg caatactggc aagagcctca ccgcggaagc caaaagtatg cagcttccag
    22621 aggtcctcac tttttgaaat tttgctcgta gcgaaccttt caagggcctt gggcaattgc
    22681 tccggcggaa tccctttgcc attatccaca accttgacat aacgaccgcc ctcagagaac
    22741 tccacagtga cagcggtggc tcccgcatca atgctgtttt ccactaactc ttttaccaga
    22801 tgcgccggtc tttcgaccac ttcgccagcg gcaatttgat ccacgacttc aggagacaag
    22861 acttcaatag acatagaact tcagtaccgc gaggggttta aaaaggaaaa ccaaaagtga
    22921 ggcttccgcc ccaatacttg gtcttttccc aatagtactt gacgttaaaa cgcagcgcgt
    22981 aggtatcagc aattcggaat gccaaaccca catcaaacac ggcgccaaaa accatatcat
    23041 caagactata ggtagcctgc tttgcatttt gcaaagcatt tagactcact tcgtaatgag
    23101 aatacttgaa catgggacca aagccaaagg tcaacatatg ccatttgctt tggggggagg
    23161 ccgtctcaaa aataaaatcc gtgagaaagg tccaaccact cgcgggaaaa cctgtacttg
    23221 tttgataata ggcaggagcc ccggaatgga acaaaaactc ggcgtcggtt cttaaatctc
    23281 catcaaacat agtattgggc cccgcgattt taactccgta aaacggcaaa gtctgcgtgc
    23341 gcaaatcgcc catagtattc tcagtgtagc ttaaataagc aagcattggc ccgcgatagc
    23401 gactggaata aaaagatttc tggggtttct ttttttcagg aaaagaatca ttttcttctt
    23461 ttttatccgg tttttttacc gtcactccct tgtcattctg aaccttcgcc gcattcttag
    23521 ggcggacatc cgtgtcggca atccaaccca caactccagg tttaacccgg actttataaa
    23581 aaggtccctt tgtcttcaaa gaaatctcat agacctgacc cgctttaaaa cgagtcaaca
    23641 ccggagcatc gaagtccgcc tctttgtata ccagagcccc ctcattgagg accacggctt
    23701 tttgcgattg agcggaagct ccccatccca caaaaaacaa aatcataaaa aaaaggcatt
    23761 gaattgcttt catatatatt taattaaaac gcatcaacca cacacgataa aaagcttcga
    23821 gcacaatttt taaagacatc ttgctgtgac ccacacgacg gtcttcaaaa acaatcggag
    23881 actcagctcc tttaaagcct tttcgcagcg ctttgtattt gagttcaatt tgaaaactat
    23941 aaccatttga aaccacagtg ctcaaatcga ttcccaggag aacttctctc ttccaggcat
    24001 taaatccccc tgtccaatcg ttcagcggaa agcctaaaat taaacgagag tagagtccgc
    24061 caccgcggga aatgattttc ctcatgactc cccaattcac cgtccggcct ccatccacat
    24121 aacgggaacc cactgcaaaa tcatgatctt ttaatttgtt aagtaaaggt cccaaatcct
    24181 caggacgatg cgagaagtcc gcatccattt ccgtcaaaac atcaaacccg ttctccatgc
    24241 cccatctgaa gcccgcgata taggcgcgac ccagcccttg tttaccagga cgagaaagca
    24301 agtgaagttg aggatggttt ttttgcatct cctggacgat ttgagccgtg ccatcaggag
    24361 agctatcatc cactaccaaa atatctacgc ccaatttttg agcaaaaact gcctgcacga
    24421 tattttgaat attttctttt tcgttgtatg taggaatgat gatgagtgct ttcatagtga
    24481 ggccagagta atctggggcc ccccaattct caatcattat ttaggacaga tttacgcttg
    24541 gcgagagacc aaaacccctt ttttatcttt actctgggct tttatttgcc tttggctgtg
    24601 cagggcctcc gccttttcag gggaaatgct tggcaagttc aagggtcttg ccaataaata
    24661 ttgaggatct aagcgccgtt gtttgagatg ttcgttcact ttctcagctg ctttatccgc
    24721 atccttcaaa tacaagactg gatcgaaacc acactgttta caggaaatcg acaagcgcca
    24781 gcgaatctgt ataaaagttt ctgtaatccc cagggccatc gcaaaaatca ccagaactct
    24841 cggatcgaag gctccccaaa aacagtacat cataagcgac gaagtcactg ccgcataaag
    24901 aatatttaag aaacctatat ttcgtttctt ataagctctt cggggagtgc gacaaaatgc
    24961 acagtaacaa ttatttttag attttagttt aaattttggt ttagatttta atattggcaa
    25021 gaattctcct acctttattc tgccggaact tgccccctct gtgaaagtcc agagccaggg
    25081 gctttttttg caaatctctc tttttctttg ttagactggc actatgacac ataaaaaatt
    25141 tctcaaactc gccagtctcg gtttgcttac tcaattcctc agtaccatgg tctgggctac
    25201 gggaaacctc ggagaaagct atcaagaagt cacctacgac gaattagtga acgaactcaa
    25261 cactaaaaaa tccacagtcg aatccaagaa gaaagcgact atcaatgaaa ttcatcttgg
    25321 ggtcggctat gtccacagtt acacccaact caacgtcaac cgcaagaaca ccgaaagatt
    25381 tcaaaatggc ttgcagctct ccgccagcat gaatttggat tctcccaacc tctacaccga
    25441 gggggtcttt cgcaatttct cgggcagcac gatcagccaa gaggatttac aaatcctgca
    25501 aattgatggc cgtctcggtt atctcaatgc gctcacggct ccctggaagt tcaatctctt
    25561 tacaggcttt tctgggcgac tcattcaagg caacaatcca gtgcaatatt attccatcaa
    25621 tgaattcact ccatctttta ctacgggact gggcatttta gcggaaattc ataaaaacat
    25681 ccggctgggc tttgaggtcg gaggtcgcac ttcgatcttg ggaaaaaata ccgaaagaaa
    25741 ctccgtcgac tttgctttgc atttggatac ttctttataa gtatggagaa cacagacgcc
    25801 ttggttattc tcttttttct tccctttctt cttttggggc tgatggtctc taccaacttt
    25861 taccgagagc ttcgccaata caattttgaa ctcaaaccca actgtctttt aacacgaaag
    25921 ccggtggttt ttatcaacgg cccttgcagc atcttttact ttcgcaaata ttggaatgcc
    25981 tatcctgaaa ttctcactga acatggctat gaagttttct cacttcatct tccatggcga
    26041 ggaccagcgc gtttcactaa aatgcagaag tttttgaatc agcaagaaat aaattcgaac
    26101 aaatatcact ttatttgtga tgaattcagt tctgcggaac tggaggggtt gtttaaatac
    26161 tcccgcagtg tggccagtct cacgatcttg aaaaacaaag aagagctttc ttctcaagat
    26221 acttattccc gcagttttct caactggagc tacaaacttc attcgtggag ttgtaagtcg
    26281 ctcgttttgc cgacggcgga ggacctaggt gtccaattcc ccacttccgc ctcgtggctg
    26341 cttgaaaaaa tgcaagaaaa tggcgagcag gactttctcg gttgaacaat caccgtaaac
    26401 aatttcatct tgccacctct gttcaataat gttgcaataa tcatcatgag agatgtcagc
    26461 gagcccacgc ccccagaaca aagagttaaa aatattctaa aatacattct gcagttttta
    26521 aaacatcctc ttcatgaaat agcccatctc cccgattggt cctggaaaac tttagcttgg
    26581 atccaagtga tttgcgcgat gatctcaggc tttgcggctg ggctgacgaa accgggcttc
    26641 tttagcattc ttgcgggcct catcgtgact ccttttattg ctatgctgat ggttttggtt
    26701 ttgaccgcat ttctttatta ctactttcaa gtcttcgaaa aaagaacagt cccagcctca
    26761 aaactttttg cgctcgccgt actttcaagt attccttttt tcatcttaca aattggctcc
    26821 agccttttgc caccgatcac gctgatcgga tttggttttt caaccatgct cattgcggtg
    26881 ggcttgactg agaactttca aatggaaaag tatcgagcta tccgcctctc catagtcctc
    26941 tttaccattg ttcttttagt ttgggtcggt aataaaataa gcgtgtaccg cctggaccgt
    27001 gcggccaacc agcaaccttt cactcttgag caataaactt gacaaaggcc tatgcccgtc
    27061 ctgtagagcc gaaaccaccg attccacgct ctgtgctgga taaagtttcc accacttcaa
    27121 actgagcttg aatcacaggt gcaatcacca attgagcgat acgctcctgg tcttgaataa
    27181 gaacgctttc atggcccaag ttgatgacaa taatcttcac ttcaccgcga taatcggcat
    27241 caattgttcc tggggtattt aaaacagtga tcccttgctt tgccgcaaag ccactgcgag
    27301 ggcgggcctg aatctcaaag cccaagggaa tttcaaagct caaacctgtc gggatcatag
    27361 cccgctcccc cggcttcaaa gtcataggag tcgttaattg cgcccgcaca tcaacaccac
    27421 tggcaccaga ggattgatac tgaggaattt cacctttgaa gttttccagt tttttaattt
    27481 ttacaatcag tgttttcata atatttcccc gacaaacgcc gttttaaccg tcgttttaaa
    27541 attcaaaatc acgccaccac ttatcgagat tttccactcc ggctcccatc aggacccctg
    27601 agaccttact ggtgtcaaac ctctcttgaa taaactcatt cactgaagcc ttcgtcactt
    27661 tttgtatttg ctgaatcacc gaatccacgg agcgatagtc cccaaaaacc atttcgttga
    27721 ccgcaataga agtcatacga ttttcaatat catcagagcc taacaaaata ctcccaatga
    27781 cttgggtctt aaaaagctcc acatcggaac tggaaatccc gctcttttta agcttccgca
    27841 attccttaga aataagttcc gccactttgc gcgcgctctt aacctcagtg ccggcgtaaa
    27901 tcaacatcgc ccctgaatcc acaaaagtat tcaacatgct gtgaatggaa taaaccaatc
    27961 cctttttctc gcgcacactt tgataaagct ttgaagtcat accaccgcct aataaagtat
    28021 tgacgatata ggcttcaaat cgttgagaat cttgaaagct cgcggttggc agtcccatca
    28081 acatatgcgt ctgctctgaa gatttctcgg acacgacccg cgctgtcttc caacgaggac
    28141 gttctctttt tgctaaaaac ttggttttct ttttcttggc aagtctttca cccaccgcat
    28201 ttaagaacgc ctgatgatct aagtttcccg ccgccgtcac tatgatgttt tttcccgtat
    28261 aacgggattt ataataatct gtcactcttt tctgcgacat gctggccaca gactctaccg
    28321 ttcccaaaat aggggtggaa gcaggatttc ccgcataggc tttttcaaaa agcacatcat
    28381 agatcaactc ttccggcgta tcatcggtca tggcgatctc ttgcaagatc accccttttt
    28441 ccaacctaaa gtcattcgtg gaaatctgca tattgctgat caaatcacac agaacatcca
    28501 aagctttttc ccaatgatct tttaagacga gggcatgata gcaggtgtat tcacgagttg
    28561 tgtaagcatt gagatcacct cccaaagcct ccaaagattt tgcgatctga taagccgaac
    28621 gagttttagt
//
LOCUS       DATBTE010000047        17353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1302927, whole genome shotgun sequence.
ACCESSION   DATBTE010000047 DATBTE010000000
VERSION     DATBTE010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 17353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17353
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302927"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VIG33_01990"
     CDS             complement(<1..463)
                     /locus_tag="VIG33_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269633.1"
                     /translation="MKLPLLIIVFFFSFSLSAKTEDAYLPVLMHSFYGTLLELRPYIE
                     SEEEFQNPKNREKIRGLLKGLENKIDQNQVKNISKAPGFAVTYGLLGSHIQETNFLFE
                     HEIYGAAHNNLKATTGLCIMCHERLPKPATLTLGEVKPKPKAVDNLSDAEFY"
     gene            complement(577..1110)
                     /locus_tag="VIG33_01995"
     CDS             complement(577..1110)
                     /locus_tag="VIG33_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEY8269634.1"
                     /translation="MKCGIFYYSYSGNNRLLAERLGERLNTTPIPILETKRRTWLTIF
                     LDVFFNRDSKIKTPDTSVLEYDLVLFIAPLWNSKIANPLKTFIKHSRKDLREYAFISF
                     CGGYEVTGQNEKVSKELYELTGRTPRAVFELHISDLFPANQRKKISVISRYKAKTEDL
                     SAFEAEIKRICEMGGFK"
     gene            complement(1119..2378)
                     /locus_tag="VIG33_02000"
     CDS             complement(1119..2378)
                     /locus_tag="VIG33_02000"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY8269635.1"
                     /translation="MQNSFTPFSKENTSNQSTDEKSGFSQVRLARMHETLRRYVDNGH
                     TPGLVTLVHHRGREYVEAMGTMAFNSNVPMREDAIFRLASMTKPITAAAALILIEECK
                     LRLDDAVDEWLPELKNRRVLKTIDSALDETVPAKRSISVRDLMTFRSGYGEVGFIAPT
                     SPLQKALMEAQLPLSVFPFADSPDEFMKRLGSLPLAFQPGEKWLYHMSAEILGVLIAR
                     VCGKSLGSFFQERIFEPLGMNDTGFYVPETKIERLPTCYGSDMFTGQLVVLDEARNGW
                     ASQAPNFEGGGGGLVSTAKDMAVFGKMLLNKGIYRNNRILSRLSIELMTSDHLTPEQK
                     ATSPFFPNFWDDHGWGLGVSVVTKRTELGGVPGRFGWDGAFGTSWFVDPKEQMVGVLM
                     TQRRPDMLAYSALVRDFWTSAYQLIDD"
     gene            2501..3154
                     /locus_tag="VIG33_02005"
     CDS             2501..3154
                     /locus_tag="VIG33_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269636.1"
                     /translation="MSDFKTIGKVGYISGSAAFIATAAYCIVQILQVVHVIRFPWDEI
                     LIYGASLCIVVPFVLAILSLHYLTPERKRFWTHASLLFSVIYSVFVIANYVVQLATVV
                     PAKLIGNIDSIRILDQTPHSLFWDYDAIGYIFMGLATLVAVPAFEKSGLEKWVRLSFL
                     AHSLVTPLIIIVYFYPTFSDKLLFLGFPWALTAPLFMFMLTLLLRKRVKALRPSLKA"
     gene            complement(3144..4205)
                     /locus_tag="VIG33_02010"
     CDS             complement(3144..4205)
                     /locus_tag="VIG33_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEY8269637.1"
                     /translation="MKERKMKALVFVAPGKIDLVEKPVPRPGPTDALVRITTTTICGT
                     DVHILKGEYPVQPGLTIGHEPVGVIEELGSAVTGYQIEQRVIAGAITPCGQCHSCFDG
                     HHSQCGGKPMGGWRFGNTIDGAQAEFLLVPNAMANLAPVPTHLTDEEVLMCPDIMSTG
                     FSGAESGKVKIGDTVAVFAQGPIGLCATAGAKLSGASLIIVVDSIEKRLRMAREVGAD
                     MTINFKEKDPVSAIMEITKGRGVDVAIEALGTEVTFQSCMRVLKPGGTLSSLGVYSKD
                     LLVPNSAFNAGLGDHHLVTTLCPGGKERMRRLMEMISHKRVELKSMVTHRFKLGEILQ
                     AYDLFANQRDGVLKVSITP"
     gene            complement(4561..4639)
                     /locus_tag="VIG33_02015"
     tRNA            complement(4561..4639)
                     /locus_tag="VIG33_02015"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            4772..5056
                     /locus_tag="VIG33_02020"
     CDS             4772..5056
                     /locus_tag="VIG33_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEY8269638.1"
                     /translation="MRGYFPKRGDFRGYLDFAENPKTQDAVAMHLQQILECTSKLSQE
                     ARGKLKINWSALVAMRNEISNSYVDVAIVWEVIKDFEEFHKLIGWARKIS"
     gene            5428..7119
                     /gene="kdpA"
                     /locus_tag="VIG33_02025"
     CDS             5428..7119
                     /gene="kdpA"
                     /locus_tag="VIG33_02025"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEY8269639.1"
                     /translation="MKPTDIAQIALYIALLIILTPILGAFMAKVFEGKKTFLSPILGW
                     SERLAYKVSGVNPSAEMNWKEYAKTLMTFNFLGFLTVFLLQILQSRLPLNPQGLPDVS
                     WHSALNTAVSFMTNTNWQGYGGETTMPYLTQMVGLTVQNFVSAATGITVFLALTRGIT
                     SKSSSNVGNFWADLTRITIYVLLPISLILAVVLVGQGVIQNFSSYTEVTTLEGIKQMI
                     PGGPAASQIAIKQIGTNGGGFFNTNSAHPFENSTPLANFLEMLAILLIPAALTFTFGR
                     MVKSQRQGWTLFAVMFFVWLAGLVGSMASEYTTNPIFHQSGIMEGKEVRLGVANSVIW
                     STATTVASNGSVNSMHSSLSPLSGGIAMFNIMLGEIIFGGVGAGLYGMLLFVLLTVFL
                     SGLMVGRSPEYMGKKIEAGEIKWAILGILAPCAVILIGTAISVVSPLGLSSLANKGPH
                     GLSEILYAFTSAAGNNGSAFAGLNANTVYYNVLLALAMLIGRFAVIIPCLAIAGLLAS
                     KKISPSSAGTFATDTPIFGFLLVGVIIIVGALTFVPALSLGPVIEHILMLQGHSF"
     gene            7129..9213
                     /gene="kdpB"
                     /locus_tag="VIG33_02030"
     CDS             7129..9213
                     /gene="kdpB"
                     /locus_tag="VIG33_02030"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEY8269640.1"
                     /translation="MEQTKSQWTNPEILIPALKDAFIKLDPRVQMKNPVMFVTEIGAI
                     LTTLLLFFGHGGEKAFEFQIAMWLWFTVIFANFAEAIAEGRGKAQAAELKKTRTTTQA
                     RVYQEGKEKKVPASQLKKGDLVICEAGDVIPGDGEVIDGIATVDESAITGESAPVIRE
                     SGGDRSAVTGGTKVISDKIIVKITAEAGHSFLDRMISLVEGAKRQKTPNEIALSIVLS
                     GLTIVFVLAVMTLKPFADYSASEAHQDLSAIITVPVLISLLVCLIPTTIGGLLSAIGI
                     SGMDRLIRRNVIATSGRAVEAAGDIDVLLLDKTGTITLGNRMATAFLSIHGVTEHRLA
                     EAAQLASMADETAEGRSIVVLAKEKYSLRGESLEKEKANFVPFSAQTRMSGVDFTDSQ
                     GIKRTIRKGAGDTVKEFVKSHSGNIPKGLDSLIETVAKKGGTPLVVADGNEILGVVQL
                     KDVVKGGIKERFAELRKMGIRTVMITGDNPLTAAAIAAEAGVDDFMAQATPEAKLKRI
                     REEQAKGHLVAMTGDGTNDAPALAQADVGVAMNTGTQAAREAGNMVDLDSNPTKLIEI
                     VEIGKQLLMTRGSLTTFSIANDIAKYFAILPALFSSLYLVQGMKEGPLQNLNIMGLSS
                     PQSAILSAIIFNALIIVALIPLALKGVKYKAGGASTVLKRNLLIYGVGGLIVPFIGIK
                     IIDILIVAVGLA"
     gene            9221..9781
                     /gene="kdpC"
                     /locus_tag="VIG33_02035"
     CDS             9221..9781
                     /gene="kdpC"
                     /locus_tag="VIG33_02035"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEY8269641.1"
                     /translation="MKYFLPSLRMLLFMTVLTGMIYPLAITGISQILFKEKANGQIIS
                     KNGISIGSELIAQKFESERYFWPRPSGVDFNSLPSGGSNLGQASSGLKKAVDERFQKL
                     KSAHPDAGEPPQDLLFASGSGLDPHISPEAARYQVARVAKARVMQIEDLVALVDKVTE
                     GRQWGILGEPRVNVLKLNLILDQIKQ"
     gene            9801..12491
                     /locus_tag="VIG33_02040"
     CDS             9801..12491
                     /locus_tag="VIG33_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HEY8269642.1"
                     /translation="MNSEERPDPDALLRAISEDEQKETRAKLRVFLGMSAGVGKTYSM
                     LRAARESMNEGIDVIIGVVETHGRKETEALLQGLPVIPKKKSVYRGTTVEEMDLDAIL
                     ARKPKLVVVDELPHSNIPGSRHEKRFQDVIEILDSGIDVITAMNVQHLESRKDAVESI
                     TGIPIRETVPDSILERASQVELVDISPSQLLKRLKEGKVYLGDKAEHAAKNFFKADKL
                     TALREIALRMTAERVDHDLQKMSGVRNESPWQTNERLLVAVSHSPYSEKLIRATRRLA
                     YNLEAPWIAIHVDTGMTLNTHDQVQLTKNLNLARQLNAEVIATTETDVPRALQRVCRQ
                     KNVTQVVVGRPTRHWFRDVLEGGSLLDRLVRESIEVDVHVIRLDGISTYKPSIRQALS
                     LYKTQIGPIKYWFTFVFLAAATGAGMGLETIVGYRTVGFLYLICVLIVGMFGSLGAVL
                     FAASLSALVWNFFFIPPKYTFSIHQPDDVILCIAYFVVAIITGLLTNRIRMHEQLMRE
                     REERTNVLYEILKDIASSQEKSEFLQKALSRVGAILGADCGVILKSPNGSLEFSEARV
                     YSIRTNEKDQAVALWSFQNRKPAGWSTDTLSESRALYIPLKGAVESVGVFVFQPLKKS
                     RRLNLEQETLLYSITGQLGISLERHFVSKRLAEAQRLKDSEQLHQTLLNSISHEMRTP
                     LTAILGTASALEDENVCKDPNAVKTLAVGLSDAGDRLNRVIENLLDMSRLSSGALSLN
                     WEWHDFNDLVGVVIQKSEKFLQRHKLKVNLSDEVKLLKMDFRLIEHAISNLILNSTMY
                     AAPNTEIKISSERQGDRFIVKIEDQGPGIPEEAVPNIFDKFYRVPGTQPGGTGLGLSI
                     VKSIIELHKGKISYENANPGARFVITLPLLAHPPLPEVVE"
     gene            12488..13168
                     /locus_tag="VIG33_02045"
     CDS             12488..13168
                     /locus_tag="VIG33_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8269643.1"
                     /translation="MSQEIRILLIDDEAAIRDIIKINLKSQGYKIDAAVSGQMGLAKA
                     VEFHPHLIILDLGLPDMNGYEVLKEFRKWTSVPIIILTVNDDEATKVRLLDAGADDYL
                     TKPFGAPELLARIRVGLRHNEFKEATPVFRSGDLEVDLNQKRVSVDGRPVRLTNTEFE
                     VLSRLVRENGKVVSQSVLLKQIWGHHAEDQSHYLRIYIKHLRTKIEKDPSKPKHIVTE
                     SGVGYRII"
     gene            13718..13894
                     /locus_tag="VIG33_02050"
     CDS             13718..13894
                     /locus_tag="VIG33_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269644.1"
                     /translation="MDNKTPKLGKKGALKEVVNLLTEIRKKPLSKVVHEIIRAENYEA
                     NLKNLKKEAYKCLP"
     gene            14022..14336
                     /locus_tag="VIG33_02055"
     CDS             14022..14336
                     /locus_tag="VIG33_02055"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HEY8269645.1"
                     /translation="MSIDKRANVIQDVIKALESGNLVPSNHAQEQMEKRDVQMSDVEE
                     MIYRAQREEAKDSLTKDKKDWKYSLRGFNDSGEKDIRIIVVFNDPDTVIVTVIDKNKK
                     ED"
     gene            14340..14801
                     /locus_tag="VIG33_02060"
     CDS             14340..14801
                     /locus_tag="VIG33_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269646.1"
                     /translation="MKTLKTYTFTGFGFDIILKDVSIKSVDGEEYPNINMNELKTDTA
                     KALLTSKQCLTGHQLKFLRTYLKMSFDEVSEKIHIPASTLRSWENKGGDFTGLSLEQE
                     KAFRILAINQILEKEKSKFNIEVTLVKEFQAPSKVAALDISSTSDSFVVNG"
     gene            15143..16390
                     /gene="thrS"
                     /locus_tag="VIG33_02065"
     CDS             15143..16390
                     /gene="thrS"
                     /locus_tag="VIG33_02065"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY8269647.1"
                     /translation="MHLKGILMSYQFDHRQLADEMEIFFFDEEIGAGLPVWLPNGVAI
                     RDQLEIFVKELERQGGYQRVVSPHLGRQDLYEKSGHLSCFNENMYPPINWQKEGDEDK
                     KSFYLKPMNCPHHHKVFSALPRSYRHLPLRIAEYGQVYRFEPSGSLRGLSRVRGLCQN
                     DAHIYVDPVDSLKEVLCVLDLHEECYKKLGLKGYRYRLSLRDPSRPNDFQGEDCKWIE
                     AESILRKALLERGLEFFEAAGEAAFYGPKIDVQMKMGSGQEESIASVQLDFLSAERFG
                     LAFSAKSGEQVCPWIIHRAPLGSHERFVALLLEYFDGQLPGWLSPIQVMLLPLSDRES
                     DECRKIHKALMKAGIRVKVDESQGSLSKRVLFAHKYRPFAKLIVGPKEISSGILKLEF
                     RDGKKEGSINSIIDSLKESCSVP"
     gene            complement(16586..16903)
                     /locus_tag="VIG33_02070"
     CDS             complement(16586..16903)
                     /locus_tag="VIG33_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269648.1"
                     /translation="MEDQNSNSDLKFKKQLASLFPNPPPAKDDQFRRIMRTYRESVEQ
                     PRWLDILGSPAAALTTAVLLVVVISFSGRQPEPPTEEENLSWLADRESSPLVEAFAPN
                     AQE"
     gene            complement(16905..17204)
                     /locus_tag="VIG33_02075"
                     /pseudo
     CDS             complement(16905..17204)
                     /locus_tag="VIG33_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
BASE COUNT         4689 a         3621 c         4093 g         4950 t
ORIGIN      
        1 aataaaattc cgcatctgat aaattatcaa ccgctttagg ttttggtttc acttcgccca
       61 gggtcaatgt cgcaggtttt ggtaagcgct catggcacat aatacacaga cccgtcgtcg
      121 cttttaaatt attatgggca gctccataaa tctcatgttc aaagagaaag tttgtttctt
      181 ggatatgact gcctaagagt ccgtaggtga ctgcgaagcc cggtgctttt gaaatatttt
      241 ttacctgatt ctgatcgatt ttattttcaa gtcctttgag aagacctctt attttttctc
      301 gatttttcgg attctggaac tcttcttcag attctatata ggggcgaagt tcaagcaagg
      361 ttccataaaa agagtgcatg agcactggta aatatgcatc ctccgtcttc gcagagaggg
      421 aaaaactaaa gaaaaatacg atgatcaaca gggggagttt cataaatacc tcacagaatg
      481 gaatctccct tcaactctgc cttatttgct cccagtaatt aacccgtttt actaaatgaa
      541 atttattcta gcaatttgtc cgggttgaga aaggaactat ttgaacccac ccatctcaca
      601 gatccttttg atctccgctt caaaagccga gaggtcctca gtttttgctt tatatcgtga
      661 aatgacgctg attttctttc gctgatttgc tgggaacagg tcgctgatgt gaagttcaaa
      721 gacggctctc ggcgtgcgtc ccgtgagctc ataaagttcc tttgagactt tttcattctg
      781 tcccgtcacc tcgtatccgc cacaaaacga gatgaacgca tattctcgaa ggtcttttcg
      841 agaatgcttg ataaatgtct tcaggggatt tgcgattttg gaattccaaa ggggcgcgat
      901 gaataggacc aggtcgtatt caagaactga agtgtcaggc gtttttattt tcgagtctcg
      961 attaaaaaat acatccaaaa aaatagtgag ccacgttcgc cttttcgttt caagaatggg
     1021 gataggggtg gtgttaagac gctcgccaag tcgttcggcc aggagtcggt tatttcctga
     1081 ataggagtag taaaatatgc cacatttcat agtcgtcctt aatcgtcgat cagttgatag
     1141 gcggaagtcc aaaaatcgcg cacgagggcg gagtaagcca gcatgtcggg gcgacgttgg
     1201 gtcatcagca ctccaaccat ttgttccttc ggatcgacga accaagaggt gccaaaggcg
     1261 ccatcccaac cgaaacgtcc cggcactccg ccaagttccg ttcgttttgt gaccacgctc
     1321 actcccagac cccacccgtg atcgtcccag aagttcggga agaagggaga ggtggctttc
     1381 tgttcggggg tgaggtgatc tgatgtcatc agctctatcg ataaacgaga aagaatgcgg
     1441 ttattgcggt aaatgccctt gttcagcaac atctttccaa acaccgccat gtccttcgct
     1501 gttgaaacaa ggccgccgcc accgccttca aaatttggcg cttgtgaggc ccatccattg
     1561 cgagcttcat ccagcaccac cagctgaccg gtaaacatat ctgatccata acaagtcgga
     1621 agtcgttcaa ttttggtctc tggcacataa aaacctgtgt cattcattcc caggggctca
     1681 aagattcgct cttgaaagaa agacccgagc gatttcccgc agacccgagc aatcaggacg
     1741 cctaagatct cggcgctcat gtggtagagc catttttccc ctggttgaaa ggccagcggc
     1801 aaactcccga ggcgcttcat gaattcgtcg ggtgagtccg cgaacggaaa aacactcaga
     1861 ggaagttgag cttccatgag ggccttttgc agaggacttg tgggagctat gaatcccact
     1921 tcaccataac ccgatcgaaa cgtcataaga tcgcgcactg agatggaacg cttcgctggc
     1981 accgtttcat caagagcgct atcgattgtt tttaagactc ttcggttctt gagttcaggc
     2041 agccattcgt cgacagcatc atcgagccta agtttgcatt cctcaatgag aatcagggcc
     2101 gcagcggcgg tgattggctt cgtcatggaa gccaggcgga aaatcgcatc ttcgcgcatg
     2161 ggcacattgc tgttaaatgc catggtgccc atggcctcca cgtattcacg gccgcggtgg
     2221 tgaaccaagg tcacaaggcc aggggtatga ccattatcaa cgtagcgccg cagggtttcg
     2281 tgcatgcggg ccagtcggac ttgcgagaag ccgcttttct catcagtgct ttgattagaa
     2341 gtattttcct ttgaaaatgg agtgaacgaa ttttgcatag aaacagtcct cgtcaaaata
     2401 agctggtgtt tcgattaaag accttgcatt aaatatgtca agaattgatt ccgtttgatt
     2461 ccgcaaattc tagagtgcta aaaaaaggga ggacaaagca atgagtgatt tcaagactat
     2521 cggcaaagtg ggatatatct cgggatcagc cgcatttatc gcaactgcgg cctactgcat
     2581 tgtccagatc ctccaggtag ttcatgtgat ccgttttccc tgggatgaaa tcctcatcta
     2641 tggggcgtcc ctttgtatcg tggtcccttt tgtcttagcc attctttcgc ttcattactt
     2701 aacgcctgaa aggaaaagat tttggactca tgcttcgctc ctcttctctg tcatctattc
     2761 ggtctttgtc atcgccaatt atgtggttca attggctacg gtcgtaccgg cgaagcttat
     2821 aggaaacatt gattcgattc ggattctaga tcagactccg cactcactgt tttgggatta
     2881 tgatgctata ggatatattt tcatggggct cgcgacactg gtcgccgttc ccgcatttga
     2941 gaaatctgga ttggaaaagt gggttcgact ctcctttctg gctcatagtt tagtgacccc
     3001 gttgatcatc attgtctatt tctatccgac gttttctgac aagctgctct tcctcggttt
     3061 cccgtgggcc ctgacggccc ctcttttcat gttcatgctc acactcttac ttcgaaagcg
     3121 agtcaaagca ttgaggccgt ctcttaaggc gtaatggaga ccttcagcac gccatctctt
     3181 tgattggcaa agagatcgta agcctgaagg atttcgccca gtttgaacct gtgggtgacc
     3241 atgctcttca attccacacg cttgtggcta atcatttcca tcaagcggcg catgcgctct
     3301 tttccgccag gacaaagggt cgtgacaagg tggtgatcgc caagccccgc attgaacgct
     3361 gagtttggaa ccagcagatc ttttgaataa acaccaaggc ttgaaagggt ccctccgggt
     3421 ttcagcacac gcatgcacga ttgaaaggtg acctctgttc ccagcgcttc aatcgccaca
     3481 tccacacctc ggcctttggt gatttccatg atggccgaaa ccggatcttt ttctttgaaa
     3541 ttaatcgtca tgtccgcgcc cacttctcgt gccattcgca atctcttttc gatgctgtcg
     3601 acaacaatga tcaagcttgc gccactcaat ttcgcgccgg ctgtggcaca aagaccgatg
     3661 gggccttggg cgaaaaccgc aacggtatcg ccgattttta cttttccgct ttcagcgcca
     3721 ctaaaacctg tgctcatgat atccggacac atcaagactt cttcatccgt aagatgagtc
     3781 ggaacgggag cgaggtttgc catcgcattc ggaactaata gaaactcagc ctgtgcgcca
     3841 tcgattgtgt tcccaaagcg ccatcctccc atgggctttc ctccgcactg agagtgatgt
     3901 ccatcaaaac acgaatggca ctgcccacag ggcgtgatcg ctccagcaat gactcgttgt
     3961 tcaatctgat accccgttac cgcggaacct agttcttcga taacaccgac cggctcgtgg
     4021 ccaatcgtca gaccgggttg aacgggatac tcgcctttta aaatgtgaac atcggttccg
     4081 catattgtgg tcgtcgtgat tcggaccaag gcatctgtcg gacctgggcg gggaacaggt
     4141 ttctcgacaa ggtcgatctt gccaggggcg acaaatacta acgctttcat ttttcgctct
     4201 ttcattgatg atctcctcca caggtcctga ggttagcatt gatatattta ctgcgaaagg
     4261 tgaggcgcga cgagacggac gtgactcttc aggtatttgg caagggcctc gccaaataag
     4321 tgagcgtccc ccggccagcg agcagaaata tagttttgat tggaccgtct cggaataagt
     4381 gcgataatga ttccttagcc aaagacaggt aaacatggtc aatgagtttt cctaaaatct
     4441 gggttggtag actctgaata attgaaacta attgtggttc ttccagaatt tgctggagga
     4501 gttgtttgtt cgaattttca gcaatacgtt gtaactatga aaaattaaag aagaaaaatt
     4561 tggcgttccc aaggggattc gaacccctgt atcctccgtg aaagtgcatc ccttaggcat
     4621 ttttaacaat ttaactaact cacaaaaacg actcatattt tcgcacttaa tctatttatt
     4681 tcagtaggtt atggttttcg actgagtccg ttttgttggt tcttgatctt ggctgttttg
     4741 ggaaagtgtg gataccagtg gatactggaa aatgagggga tattttccga aacgcggaga
     4801 tttcagggga tatttagact ttgcggaaaa tccaaaaact caggatgcag tagcgatgca
     4861 tttgcagcaa attttagaat gcacttcaaa gttatcccaa gaggccagag gtaaattaaa
     4921 gattaattgg tcggcattgg ttgcgatgag aaatgagatc tctaattcct atgttgacgt
     4981 cgccattgtc tgggaagtca tcaaagactt tgaagagttc cataagctta tcgggtgggc
     5041 gaggaagatt agctgatttt gccaaattag tattactgcc ccaagtgctg ttaaacaaaa
     5101 aatgacatct gcaaccttac ttaacaaatt cgtcagatct gggtgacggc ttagaaacac
     5161 ggttggaact tcgctctgac aaatctgagt cttcactctt ttttaaggat aagcatagtt
     5221 taggtacaga tcatcataat cctattcttt atctctttta tataattttt aagtttttta
     5281 cggattttat acggatttat attttaagat ttttaggtat tgaatggagg aacaatggac
     5341 tttgctattg cggggattat aggagttttc gttctcatat atttgggtta cgcccttttt
     5401 aaccctgaaa aattttagga aaattctatg aaaccaacag atattgcaca aattgcatta
     5461 tatatagccc ttttaattat tttgacgcct atccttgggg catttatggc gaaagtcttt
     5521 gagggcaaaa aaactttttt gagcccgatt ttgggttggt cagagcgact tgcttataaa
     5581 gtttctggtg taaacccaag cgccgagatg aactggaaag agtatgcaaa aacactaatg
     5641 acctttaatt ttttgggttt tttaactgta ttcctactcc agatacttca atcaaggctt
     5701 ccgttaaatc cgcaaggctt gcccgatgtt tcctggcact cggctcttaa tacggccgtt
     5761 agctttatga ccaacacaaa ttggcaagga tatggcggtg agacaacaat gccctattta
     5821 acccagatgg tgggtctgac tgtacagaac tttgtgagcg cggccactgg aattacagtt
     5881 tttctggcat taactcgtgg aattacttct aaatcttctt caaacgtagg aaatttttgg
     5941 gccgatctaa ctagaattac gatctacgtg ctattaccga tttcactaat tttggctgta
     6001 gtgttggtag ggcaaggtgt tatccaaaat ttctcctcct ataccgaagt gaccacgctt
     6061 gaaggtatta aacaaatgat cccgggaggc cccgcagctt cgcaaatagc tattaagcaa
     6121 attggaacaa atggtggagg atttttcaat accaacagtg ctcatccatt cgagaactcg
     6181 actcctttag caaattttct tgagatgctc gccatcctcc ttattccagc ggcactcacc
     6241 ttcacatttg gtcgaatggt caaatctcag agacagggtt ggacattatt tgctgttatg
     6301 ttttttgtat ggttggccgg tttagttggt tctatggctt ctgagtacac caccaatccc
     6361 atttttcacc aatcggggat catggagggg aaagaggttc gtctaggggt tgcaaacagt
     6421 gtgatatggt ctaccgcgac cactgtggcc tctaacggct ctgttaactc catgcactcc
     6481 agcctttctc cactttctgg ggggattgct atgttcaaca ttatgttggg tgaaataatt
     6541 tttggcggtg ttggcgctgg actttatgga atgcttcttt ttgttcttct caccgttttc
     6601 ttatctgggt tgatggtcgg cagatcacct gaatatatgg gtaaaaaaat tgaagcaggc
     6661 gaaataaagt gggcgatact cgggatcttg gcaccttgtg cggtgatttt gataggcaca
     6721 gccatttcag tagtaagccc tttaggctta tctagtctcg caaataaggg accacatggc
     6781 ttgtcagaaa ttctgtatgc gttcacttcg gcggctggaa ataatggaag tgcttttgcg
     6841 ggactcaatg caaacactgt ctactataat gtattactgg ctcttgctat gctcattggt
     6901 cgatttgcag tgataattcc ttgtcttgca atcgctggtt tgcttgctag caagaaaatt
     6961 tcaccatcat cagcaggaac ctttgcaact gacactccca tttttggatt tttacttgtc
     7021 ggcgtaatta ttattgttgg agccttaaca tttgttccgg ctttatcttt aggtcccgtc
     7081 attgagcaca tattaatgtt gcaaggtcat tctttttaga ggtgcataat ggaacaaaca
     7141 aaaagtcaat ggactaatcc tgaaatactt atcccagctc tgaaggatgc attcataaag
     7201 ttggaccctc gagttcaaat gaaaaatccg gttatgtttg taacagagat cggggcaatc
     7261 ctgacaactc ttttactctt ttttggacat ggtggcgaga aggctttcga gtttcaaatt
     7321 gcaatgtggc tttggttcac ggtgattttt gcaaattttg ctgaagcaat tgcggaaggt
     7381 cggggcaagg cccaagccgc cgaactaaaa aagacgagaa cgacaactca ggcgcgagtt
     7441 taccaagagg gtaaagaaaa aaaagttcca gccagccagc ttaaaaaagg cgacttagtt
     7501 atttgcgaag ctggagatgt catccccggt gatggcgagg ttatagatgg gattgccact
     7561 gttgatgaat cagctattac gggagagtct gctcctgtta ttcgagaaag cggcggagac
     7621 cgaagtgcgg ttactggtgg aactaaagtt atcagtgaca aaatcatagt taaaattacg
     7681 gcagaggccg gccatagttt tcttgaccgt atgatttcct tggttgaggg ggcaaaacga
     7741 caaaaaacgc ctaatgaaat tgctctgagc attgttctgt cgggacttac catcgtgttt
     7801 gttttggcag taatgactct aaaacctttt gctgactaca gtgcatccga agctcatcag
     7861 gatctttcgg cgattataac cgttcccgtt ttaatctcac ttctcgtttg ccttattcca
     7921 acaaccatcg gtggcttatt gagcgcaatc ggaattagcg gcatggatcg cctcattcgt
     7981 cggaatgtga tcgcgacaag tggtcgagct gtcgaagctg ccggagacat tgatgttctt
     8041 ctcctagata aaaccggaac aataacgctt ggcaatcgca tggccaccgc atttttgtcc
     8101 attcatggcg ttacagagca tcgtcttgcg gaggccgcac agttggcatc aatggcagac
     8161 gaaacggcag aaggacgatc catcgtcgtg ttagctaagg agaaatattc tcttcgaggt
     8221 gagagcctgg aaaaagaaaa ggccaatttt gtgccatttt cagctcagac ccgaatgagt
     8281 ggtgttgatt ttactgacag tcaaggcatc aaacgtacta ttcgcaaagg agccggtgat
     8341 accgttaagg agtttgtgaa atcccacagt ggaaatattc caaaggggtt agattccctt
     8401 attgaaaccg ttgcaaaaaa aggtggtacc cctttggtcg ttgctgatgg caatgaaatt
     8461 ttaggggtcg ttcaacttaa ggatgttgtt aagggcggaa ttaaagaacg atttgctgaa
     8521 cttcgaaaaa tgggaattcg cacggtgatg atcactggcg ataatcccct cactgctgcg
     8581 gctattgctg ctgaagctgg tgttgatgat ttcatggcgc aagcaactcc agaagccaag
     8641 cttaagcgaa ttcgagagga gcaagcgaag ggccaccttg ttgccatgac aggagatggt
     8701 accaatgatg caccggctct cgcacaagct gacgtgggag ttgcaatgaa tacaggaacg
     8761 caggcggcta gggaagctgg caatatggtc gacttagaca gcaacccaac caaactgatc
     8821 gaaattgtgg aaattggaaa acagctcttg atgacaaggg gaagccttac aacttttagc
     8881 atcgcaaacg atattgccaa atattttgca attttgccgg cactattcag ctcactgtac
     8941 ttggttcaag gaatgaagga aggtcccttg cagaacctta atatcatggg attgagttcc
     9001 ccacagagcg ctatcttgag cgccattatt tttaatgcgc tgatcatcgt cgcgctcatt
     9061 ccacttgcct tgaagggtgt taagtacaag gcgggtgggg catccactgt tttgaagcgt
     9121 aatttactca tttatggagt tggtggtttg atcgtccctt ttattggtat caaaataata
     9181 gatattttga ttgtagctgt tgggcttgcg tgaggtgctt atgaaatatt ttttaccatc
     9241 cttaaggatg ctactcttta tgactgtttt gaccggaatg atttatccgc tggcaataac
     9301 aggaatctcg caaattcttt ttaaagaaaa agccaatgga cagatcattt ctaaaaatgg
     9361 aatctccata ggctccgaat tgatcgctca gaaattcgaa tctgaaagat atttttggcc
     9421 tcgtccttca ggggtcgatt ttaactcttt accttcaggt ggatcgaatc ttggacaagc
     9481 aagtagtggc ttaaagaagg ctgttgatga gcggtttcag aaattgaaat ctgcacatcc
     9541 cgatgccggt gagcccccac aggatttatt gtttgcctct ggaagtggac ttgatcctca
     9601 tatcagtccg gaagctgcgc ggtatcaagt cgctagagtt gccaaagctc gcgtaatgca
     9661 aattgaggat ttagtggctt tggtcgataa ggtgacagag ggacgccaat ggggtatttt
     9721 aggggaacct cgagtgaatg ttttaaagct caatctaatt ttagatcaga tcaaacaata
     9781 aaagttgagg tgaccacagg ttgaattcag aagaacgccc agatccagat gctctgctgc
     9841 gggctatttc cgaggacgag caaaaagaaa ctcgcgcaaa acttcgcgtc ttcctcggca
     9901 tgtctgcggg ggtggggaaa acttactcca tgctcagggc tgcccgagag agcatgaacg
     9961 aaggaattga tgtcatcatt ggtgttgtgg agacacatgg aaggaaagaa actgaagctc
    10021 ttttacaggg tttgccggtc attccaaaaa agaaatctgt ttatcggggc actactgtcg
    10081 aggaaatgga ccttgatgct atattggcgc gaaagcctaa attagttgtc gtcgatgagc
    10141 tcccccactc caacattccc ggaagtcgtc atgagaaacg ctttcaggat gtaattgaaa
    10201 ttcttgactc tggaattgac gtcattaccg ctatgaatgt tcagcatttg gaaagccgaa
    10261 aggacgctgt cgagtccatc accggcattc caattcgtga aacggttccc gatagtattt
    10321 tggagagggc ttctcaagtc gagcttgtgg atatttctcc gtcccaacta ttaaaaagac
    10381 taaaagaagg aaaagtctat ctcggtgaca aagccgaaca tgctgccaaa aatttcttta
    10441 aagcagataa gttgactgct cttcgagaaa ttgccttgcg aatgacagca gaaagggtcg
    10501 accacgacct tcaaaaaatg tcaggtgttc gcaatgaaag tccttggcaa accaatgaac
    10561 gccttttggt tgcggtcagt catagccctt actctgagaa gttaattcga gccacaagac
    10621 ggctagcgta taaccttgag gcaccgtgga tcgcaattca tgtcgataca gggatgactt
    10681 tgaacactca tgaccaagtt caacttacta aaaatctcaa cctcgcacga caactgaatg
    10741 ccgaagtcat tgcaaccaca gaaaccgacg taccaagggc cttacaacgg gtgtgccgtc
    10801 agaaaaatgt cactcaagtt gtcgttggca ggcccacccg acattggttt cgcgatgttt
    10861 tggagggagg ctcacttctg gaccgccttg tgcgcgagag tatcgaagtg gatgtgcatg
    10921 tcattcgcct ggatggtatt tcgacttaca aaccttccat tagacaagcc ctttcccttt
    10981 ataagacaca aattggtcca atcaagtatt ggttcacctt tgtctttttg gcggcagcta
    11041 ccggcgccgg gatggggctt gaaaccattg tgggctatcg cactgttgga tttctttatc
    11101 ttatttgcgt tttaattgtc ggaatgttcg gctctttggg cgccgttctg ttcgcagcca
    11161 gtttaagtgc gctggtttgg aatttctttt ttatcccacc caagtatact ttttctattc
    11221 accaacccga tgacgtgatc ctctgtattg cctattttgt ggttgcaatc attacggggc
    11281 tcctgacgaa tagaattcgt atgcacgaac aactcatgcg agaacgtgaa gaacgtacca
    11341 atgtgcttta tgaaattctt aaagatatcg ccagcagcca agagaaatct gaatttcttc
    11401 aaaaggcctt aagtcgagtg ggagctattt tgggagctga ttgtggagtg atcttaaaat
    11461 ctccgaatgg cagtcttgaa tttagtgagg ctagagtcta ctcaattagg actaatgaaa
    11521 aagatcaagc agtggctttg tggagctttc aaaatcgaaa accggctggt tggtcaactg
    11581 acactctttc tgaatctaga gctctttata ttccgctaaa gggagcggtg gaatctgttg
    11641 gtgtcttcgt gtttcagccg ttaaagaaat cgcgaaggct taatctagaa caagaaacct
    11701 tgctttattc gattacaggt cagcttggta tttccttaga aaggcatttt gtcagcaaac
    11761 gcttggctga ggcacaacga ctcaaagatt ctgagcaact tcaccagaca cttcttaact
    11821 caatttcaca tgaaatgcgc actccgctga cggcaattct tgggacagct tcagctcttg
    11881 aagatgaaaa tgtttgtaaa gacccaaatg cggtgaaaac cctggccgtg ggattgtcag
    11941 atgccggaga tcgcctcaat cgagtcattg aaaacctcct tgatatgagt cgtttgagtt
    12001 ctggagcctt atcacttaat tgggagtggc acgatttcaa tgatctggtc ggggtggtca
    12061 ttcaaaagtc tgaaaagttt ctacaaagac ataaactcaa agttaattta agcgacgaag
    12121 ttaaacttct taagatggac tttcggttaa tagagcatgc catttcaaat ttaattctga
    12181 actcaacgat gtacgctgct ccgaatactg aaataaagat ttcctctgag cggcagggcg
    12241 acagatttat tgtcaaaatt gaagatcaag gccctgggat accggaagaa gctgtgccta
    12301 acattttcga taaattttat cgagttccag gaacgcaacc gggaggcacc ggacttggac
    12361 tatctatcgt taaaagtatc attgaacttc acaaaggaaa aatttcttat gaaaacgcca
    12421 atcctggtgc tcgttttgtt ataactctgc ccttacttgc ccacccccct ttgccggagg
    12481 tggttgaatg agtcaggaaa ttcgaattct actgattgat gatgaggccg ccattagaga
    12541 catcataaaa attaatttga aatctcaggg gtataaaatt gatgcggccg ttagcggaca
    12601 gatgggtctg gcaaaagctg tggagtttca tccccacttg attattttgg atctcggatt
    12661 gcccgatatg aatggttatg aagttctaaa agagtttcgt aagtggactt cggtgccgat
    12721 tatcattctt acggttaatg acgacgaggc tacaaaggta cgccttttgg atgccggcgc
    12781 ggatgactat ttaacaaaac cttttggtgc cccagaactc cttgcccgta tccgcgtagg
    12841 acttcgtcac aacgaattta aggaagcgac tccggttttc aggtccgggg accttgaagt
    12901 ggatcttaat caaaagcgag tttctgtgga tggccggcct gttcgcctga ccaataccga
    12961 atttgaagtg ctatcaagac tagttagaga aaatggaaag gtagtatctc agtcggtctt
    13021 attgaaacaa atctggggtc atcacgctga agatcaaagc cattatctac ggatatatat
    13081 taaacacctg cgcaccaaga tcgaaaagga tccctccaag ccaaagcata ttgtgactga
    13141 gtcgggagtg ggttacagga ttatttagaa tcgaagaacc tcacgagcct aatcacgtat
    13201 cgctattggg aaatgatcta ccgggctcaa agggaagagg caaaagatag tctgactaat
    13261 tgatggtgaa gagtacccaa acatcaatat gaatgaatta aaaactgata ctgcaaaagc
    13321 tttattaact agcaagcagc gtcttacagg tcatcagctc atattttcgc gcttaatcta
    13381 tttatttcag taagttatgg ttttcggttt ggtccgtttt gttggttctt ggtcggggct
    13441 gttttgggaa agtgtggcta ccagtggata ctggaaaatg aggtgatatt ttccgaagcg
    13501 cggagatttc aggggatatt tagactttgc ggaaaatcca aagactcaag atgcagtagc
    13561 gatgcatttg cagcaaattt tagaatgcac ttcaaaatta tcctaagtgg acagagctta
    13621 tttggttgga gtttttgcga gcgggtaaaa tcaattagaa accgcatatt gtggtttgaa
    13681 aaactatatt atattactaa agaatacgga gaatcctatg gacaataaaa cccctaaatt
    13741 aggtaaaaaa ggcgccctca aagaggttgt aaatttactt actgagattc gaaaaaaacc
    13801 tctttcaaaa gtggttcacg agatcatacg tgctgaaaac tacgaagcaa accttaagaa
    13861 tctaaaaaag gaggcctaca aatgtctacc ctaaaggctt gtctcaatgt gagaaatata
    13921 cttaataata agcgttaggc attgataaca tattaaaagt actacatttt aatatagaaa
    13981 ttgcgcattt ttaagcagtc tgataaagta gagggtgtat tgtgtcgata gataaacgag
    14041 ctaacgtaat acaggatgta attaaggctc tggaatccgg taacttagtt ccttcaaatc
    14101 acgcacaaga acagatggaa aagcgtgatg tgcaaatgag cgatgtcgag gaaatgatct
    14161 accgggctca aagggaagag gcaaaagata gtctgactaa agataaaaaa gactggaaat
    14221 attccttgcg aggctttaac gatagtggag aaaaggacat taggattatc gttgttttca
    14281 atgaccccga cactgtgatc gtaactgtaa ttgataagaa caagaaggag gactagagca
    14341 tgaaaacatt aaagacttac acattcacag gctttggatt cgacatcatt cttaaagacg
    14401 tttctatcaa atcagttgat ggtgaagagt acccaaacat caatatgaat gaattaaaaa
    14461 ctgatactgc gaaagcttta ttaaccagca agcagtgtct taccggacac cagctcaaat
    14521 ttttgcgcac ctatttaaag atgtcgtttg atgaggtttc tgaaaaaatt catattccag
    14581 cttcaactct tcgttcgtgg gaaaataagg gtggtgattt tactggccta agtttagagc
    14641 aggaaaaggc ttttcgaatt cttgctatca atcagatttt agagaaagaa aagagtaagt
    14701 ttaatatcga agtgactttg gttaaagaat ttcaagctcc gtcaaaagtc gccgcacttg
    14761 atatttcatc tacgtcagac tcctttgttg taaatgggta aactcgatta acgagattta
    14821 aaaatccaga cataacctga tctgaagatt tcgatttagc taaaatgtga gaagtgtcag
    14881 ttgttggaaa atagtctatt ttcgattaac tctcaaccac ctagtagaac ttatgggtga
    14941 cctaaattta tttgatttga gtttcaggct caaacgtcga taatagggaa tcatgtgccg
    15001 atatttacct ttcttttatt ctagtaccct tcaaaccacc ccataggggt ccatagatcg
    15061 ctttgtcccg agcacgggac caacaaatat tatttttttg cgaaatttat ttttgattga
    15121 tgcgttcaat ccttttctgg atttgcatct gaagggaatt cttatgtctt atcaatttga
    15181 tcatcggcag ttggccgatg aaatggagat ttttttcttt gacgaggaga tcggtgccgg
    15241 cttgccggtg tggctgccaa atggagtggc cattcgtgac cagttagaga tttttgttaa
    15301 agaactagag cgccagggtg gataccagcg ggtggtgagt ccccatctcg ggcgtcaaga
    15361 cctttacgag aagtcgggtc acttaagctg ttttaatgaa aatatgtatc cgccgattaa
    15421 ttggcagaaa gaaggagatg aggataaaaa aagtttctat ctgaagccta tgaactgtcc
    15481 ccatcatcac aaggttttct ctgcgcttcc aagaagctac cgtcatttgc cgttaagaat
    15541 tgctgagtac ggccaagttt accgatttga gccgagtggc tcacttcgtg ggcttagtcg
    15601 tgtgcggggg ctttgtcaaa acgacgccca tatttatgtt gatccagtgg actcgttaaa
    15661 agaagtgctc tgtgtcttgg atttgcacga agagtgttac aaaaagttgg gcctgaaggg
    15721 atatcgctat cgactgtcgc ttcgggaccc gtcaaggcca aatgactttc aaggggagga
    15781 ctgcaaatgg atcgaagcag aatctatttt aaggaaggca ttacttgagc gaggccttga
    15841 attttttgag gccgccggag aagccgcatt ttatgggccg aaaattgatg tacaaatgaa
    15901 aatgggttcg ggccaagagg aatctatcgc cagtgttcag cttgattttt tgtcagcaga
    15961 gagattcggt ctcgcctttt cggcaaagtc tggcgaacaa gtgtgtccat ggatcattca
    16021 tcgagccccc ctgggcagtc acgagagatt cgttgctctg ctattggagt attttgacgg
    16081 ccaactgcca gggtggcttt cgccaattca agtgatgctg ttgcctttgt ctgatcgaga
    16141 aagtgatgag tgtcgaaaaa ttcataaggc actcatgaaa gctgggattc gggtcaaggt
    16201 tgacgaaagt caaggctctc tatcaaagcg ggtcctattt gcgcataagt atcggccctt
    16261 tgccaaatta attgttggtc caaaggagat cagttctggc attttaaaac tcgagtttag
    16321 agatggcaaa aaggagggga gtattaatag tattatcgac agtctaaagg agtcttgttc
    16381 ggtgccatga ggagggctat cagcgacgag gttaaaagag tttgtccgcc aatcttggtt
    16441 gatgtcgcga gttggctcga atcggagaaa ggtcgtcgat tgatggccga gattgaagtt
    16501 ctttaatatg gcatagagta aaaatggggt tatgccatat taaggttctg ttttttagtt
    16561 accagtcctg aatccatcct ttaagtcatt cctgtgcatt tggtgcgaaa gcttctacta
    16621 ggggagagct ctcacgatcg gctagccatg aaagattctc ttcctctgtt ggtggctccg
    16681 gctgtcgtcc tgaaaaacta ataacaacaa caagcagcac tgccgttgtc aaagcagcag
    16741 ccggtgatcc caatatgtcc agccagcgag gctgttccac tgattcgcgg taagtcctca
    16801 ttattcgccg aaactggtca tcctttgctg gaggcggatt gggaaagagg cttgcaagtt
    16861 gttttttaaa tttaaggtca gagtttgaat tttgatcttc catattaaga cttccttttt
    16921 ttcaaaattt cagacatttg catttttgct aaattgatcc gtgatttcac agttccttcc
    16981 ggaatttgca gaatctggct gatgtcctca agcgaaagct cttcgatcga aaacagaact
    17041 attgtttcac gcagcttcgg tgagagatcc tggagaagtt tgtcaagatc atgttttgct
    17101 tctagctgat cgaaagaatt agtttgggga atctccatat tgtctttcgc ttcgtgatca
    17161 ccaaagtaac tgagccaatt tcgccttttc ctggcgcgaa gataatcatg ggctgcacgc
    17221 gccggtttaa aaaggggcca ttttcaccgg tttaccggtc ccatggtcca gggataaaac
    17281 cggaggttga ggggccattg gtggcggaat tatcatgaat aaatggaatc ccattttgat
    17341 tcagaattaa gtg
//
LOCUS       DATBTE010000048         9977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2327940, whole genome shotgun sequence.
ACCESSION   DATBTE010000048 DATBTE010000000
VERSION     DATBTE010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 9977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9977
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2327940"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VIG33_02080"
     CDS             <1..225
                     /locus_tag="VIG33_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HEY8269649.1"
                     /translation="DLMNYIRLCSRCLECNTLIQPIDKKLILDQIPPLVAEFYSVFYV
                     CPHCHKIYWEGTHFKHMQESVEQWKKELSP"
     gene            complement(234..488)
                     /locus_tag="VIG33_02085"
     CDS             complement(234..488)
                     /locus_tag="VIG33_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269650.1"
                     /translation="MQKGFNSDITVRGQKFHVQTEDWGTRNPFLVSRIFCNGAVLKTV
                     KTPYEIALKAESIREEEAIRMALRRQHTDILDVLMAGRMP"
     gene            540..968
                     /gene="mscL"
                     /locus_tag="VIG33_02090"
     CDS             540..968
                     /gene="mscL"
                     /locus_tag="VIG33_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY8269651.1"
                     /translation="MWQEFKAFISKGNVLDLAVGIIIGAAFAKIVSSFVADILMPVIG
                     LGLGKVDFTNLFIALNGEHFETLADAKKAGIPTLNYGVFLNNIIDFLLLGFAVFLIVK
                     LATKVQRKAPATATTKECRYCLSVIPLKATKCAHCGSNLE"
     gene            1085..1498
                     /locus_tag="VIG33_02095"
     CDS             1085..1498
                     /locus_tag="VIG33_02095"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEY8269652.1"
                     /translation="MTFHDLAREYFKKARIRLKALYVLNEEGGYSDVVRESQELVELL
                     LKGLLRVIGIDPPKWHDVGSILFQNKTKLGPDVQADLQRIMDLSSYLRKEREMAFYGD
                     EDFLPSDHYNKEDADYCISEVEWLLQLLTKYFEPL"
     gene            1495..1992
                     /locus_tag="VIG33_02100"
     CDS             1495..1992
                     /locus_tag="VIG33_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY8269653.1"
                     /translation="MSQSSAREIFDTCLTEWVKRVSDHFGKDLRGVLRFGSTVKDYLK
                     PETDIDMIFIFSQLPQLRREKYALLSSLEKELEALLKQKLSDHNFVLSPLLRTPEDFK
                     NFSPLYLDMVEHSVIHYDPDGLLRSTLDRTKNWIKKSGAYKVTQGTKWYWVINPTGND
                     TEIDW"
     gene            2123..3568
                     /locus_tag="VIG33_02105"
     CDS             2123..3568
                     /locus_tag="VIG33_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269654.1"
                     /translation="MNWNRAFKLHSFAQYFCILSFLLLNTASYSFAECSSADKNMVAK
                     CKDDKYSNDHYNDCNTAITKCEHSPSNDACESAKRNVENTRATYNKSCGDAGMGSTTC
                     KTKMKTCSDVGESEDFATDNNLLVAFSEALGVPQNQVGSSCPKYSGQGFFERKDKYES
                     ELKDINKDLKDNKKEIADLNKDFTADMKKIQEDIADAQKEYQELQTKTKEDQRNRATE
                     NAKIAADLAKNTRALETLMMNKSQEINTIYTERNNSLVQITEAAANRTCMAQVREERK
                     KYDAIASGSIRSQFQAASIKKKQLNDLFQECMVKFDQGRMKIIFQADQKIEALEKDIR
                     NAQSDRDAITTQLSQMNSTEAQAKQDEETTLTNAQAALQQKIQRATTELQSLQTTTQT
                     KNKATLEEQDYLTKRSKGVSNSIAALGAVPSDESSTTKISQVSGAYEDYRAAHYGALK
                     SCKNDEYTPDQIFASEGKRKSAKSGKIGGKE"
     gene            3707..6382
                     /locus_tag="VIG33_02110"
     CDS             3707..6382
                     /locus_tag="VIG33_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEY8269655.1"
                     /translation="MLKKLIIAGLALVVVGVIGAYLFFQSIKQGLPQLITVKDYHPLL
                     VSEVFDRNNKKIGEFYRQRRTLVPYDKIPKNLINAFLAAEDDQFFQHSGINYQAILRA
                     TFANLRAGRSVQGGSTITQQLAKTLLLQDTEKTLLRKIREAILAQQMEENLKKEDILY
                     LYLNQIYFGQGAWGIEVAAQTYYHKSVDKLTLPEIAILAGLPQAPTRYSPVTNPLRAK
                     ERQAYVLHRMGELGYVPKAVADEAVKKPVKVYVLETYKQHAPFFLETVRQQLVTQLGE
                     EQVLDKGLRIYTSIDLEKQIAAQESVVNGLKELDKRQGFRGALKQLSSKEEIEKFLID
                     GRKKLISDSTPERTILPDGKFAEITPQNDKQGSNTLPSYVKIGQTVEGLVEKVDDQSG
                     LVYVRVADARGLVDFADMSWARKPNTEKRWDFDILKKPSDAVKAGDIIFVKVSSETFT
                     SSRLSNPPKSKKPVVATNLPDFNKFLGLELDQEPLVEGSLLSFDEQTSDVLAMVGGYS
                     FARNEFNRALQAARQTGSSFKSIVYASALDKGFTPATPIMDSPIVFEETDQNKDDSEG
                     QEDTTKVWKPANHSKSFGGDIIFRNALVKSLNIPSVKVIEDLGITWAMDYAKRLGIFS
                     PLNPDFTLVLGSSSVTLYEMTKAFGQLGRLGKRLRPIIIHKVLDQGGQKILDEVSLDV
                     RFNKEISELDKQFDERREALAEKIKTMTPEQLAEDKKKKVDYNFFFEDKEQLIKPQTA
                     YVMTTLLKGVVEDKNGTGGRARSIGREVAGKTGTTNGYFDAWFIGYTPQIVTGVWVGF
                     DKEHSLGKGEVGGRAALPIWVEYMKTAHEGLPQMSFPVPEGIVFANIDSDTGKLASAT
                     TKNVLRQAFTEGTEPTAASNKSEDATNFLKEDLSE"
     gene            6458..9328
                     /gene="uvrA"
                     /locus_tag="VIG33_02115"
     CDS             6458..9328
                     /gene="uvrA"
                     /locus_tag="VIG33_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY8269656.1"
                     /translation="MKEIHLWGVKQNNLKNIEVKIPVGQLTVICGPSGSGKSSLAFET
                     LFAEGQRRFIESMSNYARQFLNKAPKPDIEGINNIPPAISIEQKNSVKNSRSTVGTTT
                     EVIDYLRLFFEKVGTPYCPTHKVPCEKESVTEATDKVIKFFNHKRGYILVEISADGRV
                     AEGKKLHALLLEDGYLRIYVPKKAKKTEAIEAPGSAILKEEIGTIVEISEPAAIKKGL
                     PKDRFFIVIDRLSFQDADKGRIADSLTNAYEASIKYNTNVISRRSTVLTTEGEKIQVS
                     EEASCPICNYNPPPTTSALFSFNSPIGACPTCKGFGNILEIDEKKVIPNPNLTIATGV
                     LQPFSMPSAAHERRQLNAFCKKMKIDPHTPWKDLPKAQKNMIWKGHGDFFGVKGLFEY
                     LDQIKYKMHVRVFIARYRSPFLCPDCHGARLRKEALNVFINGINIEDLTSKTIEDLGA
                     FFEKISLKPHQIEVSGEVLKQIRARLSFLIRVGVHYLTLNRETRTLSGGEYQRLMLAN
                     QLGMGLSQTLYVLDEPTVGLHPRDNDRLIEILKSLKDLGNTLVIVEHDHDVIESSENI
                     IEMGPGSGYLGGEVVYSGPTKKFYECKQSVTVPFLKHTKNLVPLQPVRPVDLANYKFK
                     IELKGAQGNNLKDLDVIFPLNRLVAVTGVSGSGKSTLVTKTLYPALARALDIEYIPAE
                     EYKDIEGVENIKNVLLIDQSAIGKSARSSPITYLKAFDLIRNLMASTQEAKVRGYTPG
                     TFSLNVDGGRCPACKGTGYEEIDMQFMDNVIIPCDVCDSKKYRPEILEIQYKGKNVYE
                     ILQMTVQEAMGFFVAHPNIRKPLSVLKEVGLDYLQIGQPANSLSGGESQRLKIAKELS
                     NVSQKATLYILDEPTTGLHFREVDLLMKVLNKLIDAGGSVIVVEHNQDVIRGADYIID
                     LGPEAGKKGGNIVAKGTPQDIIVEKKSLTGQYLKRYIESPTK"
     gene            9325..>9977
                     /locus_tag="VIG33_02120"
     CDS             9325..>9977
                     /locus_tag="VIG33_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY8269657.1"
                     /translation="MTSEISRVLSEAKPFKKTLIIIAVSGVIYALCSGMILAQLQYLV
                     DALTSRNVSGAINIGATILGLAIVVAFSRYFHIFLMNYVAECVTQNIRQKLQQKFMNL
                     SLSFHNTYASGSGGLISRILNDVKVIQDGLRMIADIFSSPLLMATLFFNLFRTNWKLT
                     LSLLLVIPVVALFQKSVSRSLRKYLPKGQENLEKMTSTIKESLDGVRVIQSFNLEKI"
BASE COUNT         3011 a         2149 c         2142 g         2675 t
ORIGIN      
        1 gatctgatga attacattcg actttgctcc cgatgtctcg aatgcaatac actgattcaa
       61 cctatcgaca aaaaactgat cttggatcag attccaccgc tggtcgctga gttctattca
      121 gttttttatg tctgtcctca ctgccacaaa atatattggg aaggaactca ctttaagcat
      181 atgcaggaaa gcgtggagca atggaaaaag gagctaagcc cttaagttct gccttaaggc
      241 attcttccgg ccataagaac atcgagaata tctgtatgct gacggcggag cgccattctg
      301 atggcttctt cttcgcgaat cgattcagct ttgagagcga tctcataggg agtttttacg
      361 gttttaagga cggccccgtt acaaaaaatt ctactgacta gaaaagggtt tctggttccc
      421 caatcttccg tttgcacatg aaatttttgg ccgcgaactg taatatctga attaaaacct
      481 ttttgcatag tacaaactct agcatagaat ctctgcataa atcaaaaggg aggttgcaaa
      541 tgtggcagga atttaaagct ttcatcagca aaggaaacgt tcttgatctg gcagttggta
      601 tcatcatcgg tgccgccttt gccaagattg tctcttcctt cgtcgctgat attttaatgc
      661 cggtgattgg tctgggtttg ggcaaagtgg acttcaccaa tctcttcata gcgttgaatg
      721 gcgaacactt cgaaactcta gccgacgcaa agaaggcagg gatccccact ttaaactatg
      781 gtgtcttctt aaataacatc atcgatttcc ttcttctagg gtttgcggtt ttcctgatcg
      841 tgaagctggc gactaaagtc caaaggaaag cgcctgcaac cgccacgact aaagagtgca
      901 ggtattgtct ttcggttatc ccgctcaaag ctacaaaatg tgctcattgc ggtagtaatc
      961 tggaataggg gctccgtcgc gctgggttga aaaaaggcga tccgtactta ttgacctatt
     1021 attgaaagaa cagcgacaat aaatcttact taaaattgtc agcactcgct ccaaaggtgg
     1081 atttatgaca tttcacgatc ttgctcgtga gtattttaaa aaggctagaa ttcgcctcaa
     1141 agcactttat gttctcaatg aagaaggtgg ctactccgat gtcgtaagag agagccaaga
     1201 attggtggag cttcttttaa aaggccttct acgagtcatt ggaattgatc ctccaaaatg
     1261 gcatgatgtc ggctcaatac ttttccaaaa taaaacaaag ctgggccccg atgttcaagc
     1321 tgatctccag cgaattatgg atctttcgag ttatttaaga aaagaacgtg aaatggcctt
     1381 ctatggtgat gaggattttc ttccgtctga ccattacaat aaagaggatg ctgactactg
     1441 tatttctgaa gtggaatggc tgcttcaact tcttacaaaa tattttgagc ccttatgagt
     1501 cagtcatcag cacgagagat ttttgatacc tgcttaacag aatgggtgaa aagagtcagt
     1561 gatcatttcg gcaaagacct acgtggagtt ttgcgattcg gttcaactgt aaaggactat
     1621 ctgaagcctg aaacagatat tgatatgatc tttatttttt ctcagttacc acaactaaga
     1681 cgtgaaaagt atgccctgct ctcttctctt gaaaaagaac tcgaggcttt attaaaacaa
     1741 aaactttctg atcacaattt tgtactttca ccgcttctaa gaacgcctga agattttaaa
     1801 aattttagcc ctctttatct cgatatggtt gagcattcgg tcattcatta tgatccggat
     1861 ggactgctga gaagcacctt agacagaaca aaaaattgga ttaaaaagtc tggtgcttac
     1921 aaagtcacgc aggggaccaa atggtactgg gtcatcaatc ctacaggcaa tgatactgag
     1981 attgattggt gagtttaatt aacactatca gatgcccgac ataggtcgta tcaaactgag
     2041 actaatcttt tatgttatta aaaatacctg cataataagg aatcaatttt gcatcttatg
     2101 accttatctg ggaggatctc ttatgaattg gaatagagcg tttaaactcc attcatttgc
     2161 tcaatacttc tgcattctta gctttctgct tttaaatact gcttcctact ctttcgcaga
     2221 gtgcagctca gcagataaaa acatggtcgc caaatgtaaa gatgataaat acagcaatga
     2281 tcattataat gactgcaata cggcaattac caaatgtgag cactccccaa gcaatgacgc
     2341 ctgtgaatcc gcaaaacgca acgtagaaaa tacgagagcc acctataaca aatcctgcgg
     2401 tgacgccgga atgggcagta caacttgcaa aacaaagatg aaaacctgtt ccgatgtggg
     2461 agaaagtgaa gactttgcca ctgacaacaa tttattggtg gccttcagtg aagctctggg
     2521 agttccccaa aaccaagttg gctcaagttg tccgaagtac tctggccaag gcttcttcga
     2581 aagaaaagat aagtatgaaa gtgaacttaa agacatcaat aaagatctta aagataacaa
     2641 aaaagaaatt gcggatctca ataaggattt cacagcggat atgaaaaaga tccaagaaga
     2701 catcgctgat gctcaaaaag agtatcaaga actccagact aaaactaaag aggaccagcg
     2761 aaatcgcgct actgaaaatg ccaaaatagc tgcggattta gctaagaaca cgcgtgcctt
     2821 agaaacccta atgatgaaca aaagtcagga aatcaataca atttacactg aaagaaacaa
     2881 ctccttggtt caaatcaccg aagccgcagc caaccgaact tgtatggctc aagttcgtga
     2941 ggagcgtaaa aaatacgacg ccatcgcctc tggcagcata cgatctcaat ttcaagcggc
     3001 atcgattaaa aagaaacagt taaacgatct tttccaagaa tgcatggtta agttcgatca
     3061 aggacgcatg aaaatcattt tccaagcaga ccaaaaaatt gaggccttag aaaaagacat
     3121 tcgaaacgcg caatctgacc gtgatgcgat aactacccag ttatcccaaa tgaactccac
     3181 agaagcgcaa gctaaacaag atgaagaaac tactttgacc aatgctcaag ctgcgcttca
     3241 acaaaaaatc caacgagcta ctactgaact tcaaagctta cagaccacca cccagactaa
     3301 aaacaaagcc acccttgagg agcaagatta tcttacgaaa agatccaaag gagtttccaa
     3361 ttctattgcc gccttgggcg ccgtgccaag cgatgagtca agtaccacaa aaatttctca
     3421 agtttcagga gcctatgaag attatcgagc cgcacattat ggagcactca agtcctgtaa
     3481 aaatgatgaa tacacccctg atcagatttt cgcttcagaa ggaaaaagaa aatccgcgaa
     3541 aagcggaaaa atcgggggaa aggaatagtt gctagcctga agactagctg aatcttcctg
     3601 agcccccaca aaggtctttt ttcattgtag accctcccac gtcacgctgc taaaatgatg
     3661 gctctttgta aaattataga gtccatctgt ttaaggagct gcctccgtgc taaaaaaact
     3721 tattattgcg ggtcttgcct tggttgtagt tggtgttatc ggggcttatc tgttttttca
     3781 atcgatcaaa caaggtttgc ctcagttaat cacagtgaaa gattatcacc ctctcttggt
     3841 gtctgaagtt tttgatcgaa ataataagaa aatcggcgaa ttctaccggc aacgtcgcac
     3901 tttggtacca tatgataaaa ttcctaaaaa tctcattaat gctttcttgg cggctgaaga
     3961 tgatcagttt tttcaacaca gtgggataaa ttatcaagcg attctgcgtg ccacctttgc
     4021 caatcttcgt gctgggcgct cggtgcaagg gggctccaca atcactcagc agctcgcgaa
     4081 gacattactt cttcaagata ccgagaaaac cctgctgaga aaaattcgcg aagccatatt
     4141 agctcaacaa atggaagaga atctgaaaaa agaggacatt ctgtatttgt atctgaatca
     4201 aatttatttc ggacaaggag cttggggtat tgaagtggcg gcacaaactt attatcacaa
     4261 atctgtggat aagctgacac ttccggaaat agccattctt gctggactac cgcaagctcc
     4321 gactcgttat tctccggtca ctaatcctct tcgtgcaaaa gaaagacaag cttatgttct
     4381 tcaccgcatg ggggagttgg gatatgtgcc caaagcggtg gctgatgagg ccgtaaaaaa
     4441 accggtaaaa gtttatgtgc tggagaccta caaacaacat gcacctttct ttctcgaaac
     4501 tgtgcgtcag caattagtga ctcaattagg agaagagcaa gttctagata aaggtcttcg
     4561 catttacacc agcattgatc ttgaaaaaca aattgcggct caggagtccg tggttaacgg
     4621 cctgaaggaa cttgataaac gacaggggtt ccgtggagct cttaaacaac tttccagcaa
     4681 agaagaaatt gaaaagtttc tgatcgatgg tcgtaaaaaa ttgatttcgg attctactcc
     4741 agaacggacg atcctgccag atgggaagtt tgctgaaatt actccccaga atgataagca
     4801 gggctccaat actctgcctt cttatgtgaa aataggtcag actgttgaag gcctcgtgga
     4861 aaaagtcgac gatcaatcag ggcttgtcta tgtccgcgta gcagatgctc gcggcctggt
     4921 tgacttcgcg gacatgtctt gggcgcgcaa acccaatact gaaaaacgtt gggacttcga
     4981 cattctcaaa aaaccctctg atgctgttaa agccggagat atcatctttg ttaaagtcag
     5041 tagcgaaacc tttacctctt cacgtcttag caatcctcct aaaagtaaaa agccagttgt
     5101 tgcgacaaat cttcctgact ttaataaatt cttaggtttg gaattggatc aagagccgtt
     5161 ggtggaaggc tcgctgcttt cttttgatga acaaacctct gacgttttag ctatggttgg
     5221 gggatacagc ttcgctcgaa atgagtttaa tcgtgctctg caagccgctc gtcaaacagg
     5281 ctcctctttt aagtccattg tttatgcctc ggctttggat aaaggtttta ctccggcgac
     5341 tccgatcatg gattctccga tcgtgtttga agagactgat caaaataaag atgatagcga
     5401 aggacaagaa gacacaacga aagtttggaa gcctgcgaat cattcaaaaa gtttcggcgg
     5461 agatatcatc ttccgcaacg ccttggtgaa atctttaaac attccttccg tcaaagtcat
     5521 agaagacctg ggcattacct gggctatgga ttatgcaaaa cgccttggta tttttagtcc
     5581 tctgaatccg gatttcactt tggttctggg ttccagtagc gtgactttat atgagatgac
     5641 gaaagccttt gggcaactcg gtcgacttgg aaaaagatta aggcctatta ttatccataa
     5701 agttcttgat cagggtgggc aaaaaattct cgatgaagtc agtctggatg tgcgttttaa
     5761 taaagagatc tctgaactcg ataagcaatt cgatgagcgt cgagaggccc tcgcggaaaa
     5821 aattaagact atgactcctg agcagcttgc tgaagataaa aagaaaaaag tggactacaa
     5881 tttctttttt gaggacaaag aacaattgat taaaccccaa accgcttatg tcatgactac
     5941 tttattaaaa ggcgttgtcg aggataaaaa tggcacgggt ggccgggcac gctctattgg
     6001 tcgtgaggtc gctggcaaaa ctggaacgac caatggctat ttcgatgctt ggttcatagg
     6061 ttatactcct caaattgtca ccggagtatg ggtcggcttc gataaggagc acagtctcgg
     6121 caaaggcgaa gtgggaggac gtgcggctct tcctatttgg gtggaataca tgaaaaccgc
     6181 ccacgagggg cttcctcaaa tgagcttccc cgtccctgag ggcatagtct tcgccaatat
     6241 cgacagcgac acaggaaagc tcgcctcagc gaccacaaaa aatgttctga gacaagcttt
     6301 cacggaaggc accgaaccca ccgcagcttc caataaatca gaagatgcca ccaatttcct
     6361 aaaagaagac ttatccgaat aggaggatga cattcctaaa ggtgcagagt actctctggt
     6421 ttgatcacca gaaactttgt aaacgaggct tgtctaaatg aaagaaattc acctttgggg
     6481 ggtgaaacaa aataacctga agaacatcga agtcaaaatt cctgtgggac agctcacggt
     6541 gatttgtggc ccttcgggtt ctggcaaaag ctctttggct tttgaaacgc ttttcgctga
     6601 aggacaacgg cgcttcatcg aaagcatgtc gaactacgct cgacaattcc ttaataaagc
     6661 cccgaaaccg gatattgaag gcatcaacaa tattccaccc gccatttcca ttgaacaaaa
     6721 aaactcagtt aaaaatagcc gctccacggt tggcactacg acggaagtca tcgattatct
     6781 gcgtttgttc tttgaaaaag ttggaacacc ttattgccct actcataaag ttccttgtga
     6841 aaaagaaagt gtcactgaag caaccgacaa ggtgattaag ttctttaatc ataaacgtgg
     6901 ttatattctg gtggagattt ccgccgacgg tcgcgtcgcc gaaggtaaaa aactccacgc
     6961 gcttttatta gaagatggat acttacgtat ttatgttcca aagaaagcca aaaaaactga
     7021 agccattgag gcgcccggct ctgcaattct taaagaagaa ataggaacga tcgttgagat
     7081 tagtgagcct gcagctatca aaaaaggcct tcctaaagat cgcttcttca tagttattga
     7141 tcgcttgtct ttccaagacg ctgacaaagg acgtatcgct gattccctga ccaatgccta
     7201 cgaagctagc atcaaataca atacaaatgt aatttcacgc cgctctacgg ttctgacgac
     7261 ggagggggaa aaaattcagg tcagcgaaga ggcttcgtgt cctatttgta attacaatcc
     7321 tccacccaca acttcagccc tttttagttt taatagcccc attggcgcct gccccacttg
     7381 taagggtttt ggtaatattc ttgaaatcga cgaaaagaaa gtcatcccga atccgaacct
     7441 cactattgca acaggcgttc ttcagccctt ttcgatgcca agcgccgcac atgagcgccg
     7501 ccagctcaac gccttctgta aaaaaatgaa gatcgatcct cacacgcctt ggaaggattt
     7561 acccaaggcg caaaaaaata tgatctggaa gggtcacggc gacttctttg gcgtaaaggg
     7621 tctttttgaa tatctcgatc aaattaagta caaaatgcat gtgcgggtct ttattgctcg
     7681 ctaccgcagt ccttttttat gtcctgattg ccatggagcc agattacgta aagaagcttt
     7741 gaatgtgttt attaacggga tcaatattga ggatctgact tcaaaaacaa ttgaagactt
     7801 aggggccttc ttcgaaaaaa tctctttaaa gcctcatcaa atcgaagttt ctggcgaagt
     7861 tttaaaacag atccgtgctc gtttgagctt tttgattcgc gtgggtgttc attacctgac
     7921 tttaaaccgt gaaacacgaa ctctctccgg cggagaatat cagcgtttaa tgttagccaa
     7981 tcagctggga atgggacttt cacagacact ctatgttctc gatgaaccca cggtagggct
     8041 tcacccccgg gacaatgatc gcctgattga gattttaaaa agccttaaag atttaggcaa
     8101 tactctcgtt attgtcgaac acgatcatga tgtgattgaa agcagcgaaa atatcatcga
     8161 aatgggacct ggctcgggat atcttggcgg tgaagttgtt tattccggac cgacgaagaa
     8221 gttctacgaa tgtaagcagt ccgttacggt tccctttttg aagcacacta aaaacttggt
     8281 gcctttacaa cctgtacgcc cggtagatct ggcaaactat aaatttaaaa ttgaactcaa
     8341 aggagcccag ggcaataacc tgaaggacct tgatgttatc tttcctttaa atcgtttggt
     8401 cgccgtaact ggcgtgagtg gatccggaaa gtcaacttta gtgactaaga ctctataccc
     8461 cgctctcgca cgcgctttgg atatcgaata tattcccgct gaagaatata aagatattga
     8521 gggcgtagag aatataaaaa acgtactcct gatcgatcaa tcagcgattg gaaaatcggc
     8581 acgaagttct ccgattactt atctcaaagc ctttgatctt attcgtaatc ttatggcctc
     8641 cacccaagaa gcgaaagtcc gaggttatac tcctggaacc tttagcttga acgtggacgg
     8701 aggtcgttgc cccgcttgca agggaacagg ctatgaagag atcgacatgc aattcatgga
     8761 caacgtgatt attccttgcg acgtttgcga tagtaaaaag taccgccctg aaatcctgga
     8821 gatccagtac aaaggaaaaa atgtctatga aattctgcaa atgaccgttc aagaggccat
     8881 gggcttcttt gtggcccacc ccaatattcg taagcctttg agcgtgctta aggaagttgg
     8941 tttagactac ttgcaaatcg gtcaacccgc caacagcttg agcgggggag agtcccagcg
     9001 tcttaaaatt gctaaagaac tttccaacgt aagtcaaaag gcaaccctct acattttaga
     9061 tgaaccaaca accggtcttc atttccgtga agtcgatctc ttgatgaaag ttttaaataa
     9121 acttattgat gctggaggca gcgtcatcgt ggttgaacac aatcaagacg ttatccgcgg
     9181 agccgactat attattgatc tcggtccaga agctggtaaa aaaggcggca acatcgtcgc
     9241 taaaggaacg cctcaagata ttattgttga aaagaaaagt ctcacaggac aatacctgaa
     9301 acgctatatc gaaagcccca ctaaatgacg tctgaaatat cccgtgtctt gagtgaagca
     9361 aagccattca aaaaaactct cataatcatc gctgtgtcag gagtgatcta tgccctttgc
     9421 tccggtatga ttttagcgca gctccagtat ctggttgatg ctctaacttc aagaaatgtt
     9481 tcaggggcga taaatatcgg cgctacaatt ctaggattag ccattgtcgt tgcattcagt
     9541 cgctactttc atatattcct catgaactat gttgctgaat gcgtgactca aaatattcgt
     9601 caaaagctgc aacaaaaatt catgaacctc agcctttcat ttcataatac atatgcctcc
     9661 ggctcgggtg gcttgatttc aagaatttta aatgatgtga aggtaattca agacggtttg
     9721 cggatgatcg ccgatatctt cagttcccct ttattgatgg caacgctttt ctttaatctc
     9781 ttccgcacga attggaaact caccttaagc ctgcttttag tgattccagt tgttgcactt
     9841 ttccagaaaa gcgtgtctcg cagtctgcgt aaatatttac ctaaaggaca agaaaatcta
     9901 gaaaagatga cttcaaccat caaagaatct ctagatgggg tccgggtcat tcagtctttc
     9961 aatttagaaa aaataat
//
LOCUS       DATBTE010000049         7722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_551803, whole genome shotgun sequence.
ACCESSION   DATBTE010000049 DATBTE010000000
VERSION     DATBTE010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 7722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7722
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551803"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1889)
                     /gene="lon"
                     /locus_tag="VIG33_02125"
     CDS             complement(<1..1889)
                     /gene="lon"
                     /locus_tag="VIG33_02125"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY8269658.1"
                     /translation="MSENNKVQQLPLLPLRDLIIFPHMMMPLFVGRDKSINALEEAMS
                     KQTDIVLAAQKDAKTNTPEPKDIYSIGTIATIIQLLRIPDGTVKVLVEGKRRVRIKNF
                     IPNDNFFQVSCEEIPEEAESPVEAAALVRSVKTTFETYVKLNKRIPPEILMRVSTIES
                     PGELADIIVAQLNLKLEDKQKVLEISDPSKRLEHLLNLMTGEIEILEVEKKIRTRVKK
                     QMERSQKEYYLNEQMQAIQKELGEKDDYQAELQELELKTKAKKMNQDAKDKVMKEIKK
                     LKMMSPMSAEATVVRNYIDWVLSLPWSDYSDEKHDIRNAQKVLDDDHWGLEKVKERIL
                     EYLAVLSISKNMKGPILCLVGPPGVGKTSLAKSIAESMNRPFARISLGGVRDEAEIRG
                     HRKTYVGAMPGKILQALRKVDKGNPLVLLDEIDKMASDFRGDPASAMLEVLDPEQNGT
                     FQDHYLELEYDLSKVMFIATANTLHTVPRPLLDRMEIISLEGYIEQEKFHIAKNYLVP
                     KQIENHGLKEYKVSIPDKTIRDVIRFYTREAGVRNLERQLANVCRKVAKDIVMSQAIA
                     EMKGGAKKSTKKETSKAGVGKISTYVVSPQKLVELLGPHKFKTSKIEEQNEIGLTNGM
                     AWTEVG"
     gene            complement(2194..3495)
                     /gene="clpX"
                     /locus_tag="VIG33_02130"
     CDS             complement(2194..3495)
                     /gene="clpX"
                     /locus_tag="VIG33_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY8269659.1"
                     /translation="MSTKDAGTLRCSFCGKGQKEVKKLIAGPGVYICDECIDLCNDII
                     DEEKDRETSIKGTFKVPKPHDIKTYLDDYVIGQLKAKKALAVAVHNHYKRINALGKGK
                     KGTDVELQKSNILLVGPTGSGKTLLAQTIAKILNVPFAMADATTLTEAGYVGEDVENV
                     VLNLLQSADYDVEKAQKGVIYIDEIDKISRKSENPSITRDVSGEGVQQALLKILEGTV
                     ANLPPKGGRKHPQQEFIQVDTSNILFIVGGAFVGLDKVIENRTTNKTMGIGADIRTAG
                     EKDTAGEYILQKVEPDDLTKFGLIPEFIGRLPVIAVLNPLEESALIDILVQPKNAVTK
                     QYQKLFSYEGVDLKFTEKALKAIAQMALKRKTGARGLRGVIESAMMDIMFDLPSKENV
                     KECIIDEKVINEGAAPKLVYKTEDEMRAEATKSENDPAESA"
     gene            complement(3499..4119)
                     /locus_tag="VIG33_02135"
                     /pseudo
     CDS             complement(3499..4119)
                     /locus_tag="VIG33_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166059.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
     gene            4273..5778
                     /locus_tag="VIG33_02140"
     CDS             4273..5778
                     /locus_tag="VIG33_02140"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEY8269660.1"
                     /translation="MLFDLINKDIETLTCPALVVFSKAPSQKDKSAKMTHSELGKKLT
                     EAFEEKTITGKHLETVLYRELNYKNFRHVLVVGLGKESDMTHETVRQSIAAAFEALKN
                     AGVKEAALHFDGLTTNKKEIATYAKATAEGLALTSYVFDELKTSGKKEPKENDLTLHV
                     VAKDSDKAFKAAFSEGVTLGTCVNFSRRLGDLPGNLMTPTNLADSAVEAAKGSGLKVT
                     VWDKARIKKEKMGGLLGVSNGSSEEPRFIIMEYNGTAKTKKPVVFVGKGLTFDCGGIS
                     IKPSAGMEEMKYDMCGGANVIGTLLAIARLKLKVNVIGLVASTENLVGPGATKPGDVH
                     TARNGKTFEVNNTDAEGRLILADALSYATELEPQIIIDAATLTGAMVVALGNIHTGYF
                     TRNNVLKGKVEKAADASGELVWNMPLTDHHVKDMKGTFADLSNMSSGKGAGSATAAAF
                     LEQFVGEGIPWAHFDIAGTGWAVGNRLNYCPKKGASGAMIRTFIEIAKQYV"
     gene            5880..6986
                     /locus_tag="VIG33_02145"
     CDS             5880..6986
                     /locus_tag="VIG33_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY8269661.1"
                     /translation="MGPIYVWQKSDWPNFIWDSEQLLEDLGQARKLQGKILGFANFVG
                     LEAQANLLAEEAMTTSAIEGEKLNKDSVRSSVARRLGLSTSGLPPQQREIDGLIEMLM
                     DATSNHQSRLTAKRLFGWQAALFPTGFSGLRKIAVGTWRKGTQPMQVISGRLGKEKIH
                     FEAPPSSQVAKEMKAFFDWWEGPSKKLDGIIRAGIAHFWFVTIHPFEDGNGRIARAIT
                     DMALAQDEQTGCRFYSLSSQIVFERNAYYDILEASQKKNSDLTLWLKWFLKMFCLAIE
                     KSEGVVAKALNIARFWQLHNQAEFNSRQIKIINRLLEAEPEGFEGGMTNRKYVGLTKV
                     SRETAKRDIAELEEKGVLKRNSGKGRSISYSLSF"
     gene            complement(7021..7656)
                     /locus_tag="VIG33_02150"
     CDS             complement(7021..7656)
                     /locus_tag="VIG33_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269662.1"
                     /translation="MKKLGMTFLLFLSSWGVGYGIMLHTSNWGPINRDPAAVRQIYDF
                     SNLSGTDLTDAMKKRIISGSTVIRKNQSLGVELGHFAMAKITGEKTLACQEFQKVIFR
                     FEAEGMAIGGERPAMEVEGACEFSEDMTKISPIFIPVDKVLAEKPADGEFQYREGKPV
                     TIRVHGMPSEWPTRWLLTSVRLSNSSEQRELLINSDEVSEILGRPMMVYFK"
BASE COUNT         2126 a         1772 c         1606 g         2218 t
ORIGIN      
        1 cccacttccg tccaagccat accattggta agaccgatct cattttgttc ttcgatctta
       61 ctggttttaa acttatgcgg ccccagtagc tctaccaatt tttgcggtga tactacgtag
      121 gtgcttattt ttcccactcc agcttttgat gtctctttct tagtggactt cttggctccg
      181 cctttcatct ccgcgatggc ctgagacatc acgatatcct tcgcaacttt gcggcaaaca
      241 ttggcgagtt gtctttctaa attacgcaca cccgcttcac gagtatagaa acgaatcacg
      301 tcgcgaatgg tcttatccgg gattgaaacc ttatattctt tcaatccatg attctcaatt
      361 tgcttcggca caagataatt cttagcaatg tggaactttt cttgctcgat gtatccttca
      421 agactgatga tctccatacg atctaaaaga ggtcttggaa cagtatgcaa ggtattcgca
      481 gtcgcaatga acatgacttt tgaaagatca tattccaact ctaagtagtg atcttggaaa
      541 gtcccgtttt gctcgggatc taaaacctcc aacattgccg atgctggatc cccgcggaag
      601 tcagaagcca tcttgtcgat ttcatccaac agaaccaaag gattgccttt atctactttg
      661 cgaagtgcct gtaaaatttt acctggcatc gcgccaacgt aggtttttct atgaccgcga
      721 atttccgcct catctcttac gccgcccaaa gaaatgcgtg caaagggacg gttcatagac
      781 tccgcgatgg atttggccag agaggtctta cccacccctg gaggaccaac tagacaaaga
      841 ataggtccct tcatgttttt tgaaattgaa agaaccgcca agtactcaag aattctttct
      901 ttaactttct ccagccccca atggtcgtca tcaagaactt tttgagcatt tctaatgtca
      961 tgcttttcgt cgctataatc agaccatggc aaggataaaa cccagtcgat gtaattgcgg
     1021 acaacggtgg cttccgccga catcggggac atcatcttga gttttttaat ctccttcatg
     1081 actttgtctt tagcatcttg gttcattttc ttagctttag tctttaactc taattcttga
     1141 agctccgctt ggtagtcgtc tttctcccca agttcttttt gaatggcttg catttgctcg
     1201 tttaaataat actctttctg agaacgctcc atttgctttt taactcgggt gcggatcttt
     1261 ttctctactt cgagaatctc aatctcacca gtcatgagat tcaacaaatg ctccaaacgt
     1321 tttgaaggat ccgagatctc taaaaccttt tgtttatctt caagtttaag attgagctgc
     1381 gccacaataa tgtccgcaag ctctcccggg ctttcgatag tagacactct catcagaatt
     1441 tctggaggaa ttcttttatt gagttttacg taagtttcaa aagttgtttt tacagaacgc
     1501 acaagtgcgg ctgcttcaac tggactttcg gcttcctctg ggatctcttc acaagaaacc
     1561 tggaaaaaat tatcgttcgg tatgaaattt ttaatacgaa ctctgcgttt accctcgact
     1621 aagactttta ctgttccatc tggaattctc aaaagctgta taatagtggc gattgttccg
     1681 attgaataaa tatctttagg ctctggagta ttcgtctttg catctttttg agccgcaagg
     1741 acaatatccg tttgtttact catagcctct tcaagagcgt taatactctt atcgcggcct
     1801 acgaacaaag gcatcatcat atgcggaaaa atgatgagat ctcttaaagg caatagtgga
     1861 agctgctgta ccttgttgtt ctcactcatt ttaatgaccc tccctgtgtg gtgttttggt
     1921 atactctgag tctgtaacta agaaccccga gaaaaagtaa ggggttaaat cctttaacca
     1981 ttcacttctt cattaagtat aaatatgtgt acggtttcag ggaagtaaat tagtgaagtc
     2041 cctttttttc tttaggaaaa cgaaaaaacc cagactccat caacgcatag agataatcat
     2101 gatttgaata agaatttctt atatattggg atcagcaata aaaaaaccga agaatttctt
     2161 cggttttttc atcacgattt gacaagttgg attttaagcg ctttcggccg gatcgttttc
     2221 ggatttcgta gcttccgcgc gcatctcgtc ctcggtcttg tagaccaatt taggtgcggc
     2281 cccttcattg attactttct catcaataat acactctttg acattctctt tggaaggaag
     2341 gtcaaacata atgtccatca ttgctgattc aataacacct cgcaagccac gagctccagt
     2401 tttgcgcttt aaagccatct gcgcaatcgc tttcaaggcc ttttctgtga atttaagatc
     2461 gaccccctca tagctgaaaa gtttttgata ttgttttgta actgcattct ttggctgcac
     2521 caaaatatct atcaacgctg attcctctaa aggatttaac acagcgatca ctggtaaacg
     2581 accgataaac tctggaatca aaccaaactt tgtcaaatca tcgggctcaa ctttctgtag
     2641 gatgtattct cccgccgtgt ccttttctcc cgcggtacga atgtccgcgc caatgcccat
     2701 ggttttattc gtcgtacggt tctcaatcac tttgtccaat ccgacgaaag ctccccccac
     2761 gatgaagagg atattcgaag tgtccacttg gataaattct tgctgaggat gcttacgacc
     2821 acccttcgga ggcaggttcg ccacagttcc ctcaagaatt tttaaaagtg cttgttgcac
     2881 gccttcacca ctcacgtcac gagtgataga aggattttca gatttacgag agatcttatc
     2941 gatctcgtcg atataaataa cccctttttg agctttttcc acatcgtaat ccgcagattg
     3001 aaggagattt aaaactacgt tttccacgtc ctcacccaca tagccggctt cggttaaagt
     3061 ggtcgcatcc gccattgcaa atggaacatt caagatcttt gcaatcgttt gcgccagcaa
     3121 agtcttgcct gaacccgtgg ggcctaccaa taaaatattg gatttttgca actcaacgtc
     3181 cgtccctttt ttacctttgc ctagggcgtt gatacgcttg tagtgattgt gcacggcaac
     3241 cgccaaagcc tttttcgcct tcagctggcc aataacgtaa tcatccaaat aggttttaat
     3301 atcatgcggt ttggggactt taaaagttcc cttaatggag gtctctcggt ctttttcctc
     3361 gtcgatgatg tcattacaga ggtcaataca ctcatcgcaa atataaacgc caggaccggc
     3421 aattaatttt ttaacttctt tttggccttt accacaaaaa ctacatctta aagtgcctgc
     3481 atctttagtg ctcattgatt atcctttttt agaagcctta cgagattcaa caatggtatc
     3541 aatcagtccg agctctttag cctccgccgg agccaagaaa taatctcgct ccatcaactt
     3601 tgccagagtt tcatattttt gacccgtgtg ggtttcgtaa attttcgtca acttctcttt
     3661 ggtttttatc aattctttgg cgtggatttc aatatccgtt acctgacccg aaagtcctcc
     3721 accagacaac aaaggctgat gaatcatgat gcgtgtattg ggaagactaa agcgtttgcc
     3781 cgctgatccc gccgacaaaa gcaaagagcc catgcttgcc gccatgccca tacaataagt
     3841 cgccacgtcg catttcacaa actgcataaa gtcataaata gccaagccag ctgatacgct
     3901 gccaccagga gagtttatat aaagatgaat gtccttctca ggattgtcca cctcaagaaa
     3961 caaaaactgc gcaataatgc tgttggcgac ttcatcagtt acctgagtac caagtataat
     4021 gattcgatct ttaagtaagc gcgaatagat atcataactt ctttcgccct tagaggtctg
     4081 ctctacaacg taaggaataa gtgccacagt ggtctcctgt tgggggttaa ctccatcttc
     4141 ttatctatcg gtattataat aaaggacttt attaaaaagg ctttaatggc atttgcgttt
     4201 gtgttagtga tttaacggtc acatcttaag ccactaacgc gttacattaa gttgttgttg
     4261 aaaggacatc ttatgctatt tgacctcatt aacaaggata ttgaaacctt aacctgccca
     4321 gctttggtcg tgttctcgaa agctccatct caaaaagata aatcagcgaa aatgactcat
     4381 tctgagctgg gaaaaaaact aacagaggcc ttcgaagaaa aaaccatcac tggtaaacat
     4441 cttgaaactg ttctttaccg cgaattaaat tataaaaatt tccgccacgt ccttgtagta
     4501 ggtctaggta aagagtcgga catgactcac gaaacagttc gtcaatccat tgccgctgcc
     4561 ttcgaagctc tgaaaaatgc aggagtgaaa gaggccgcgc ttcactttga cgggctgaca
     4621 acaaacaaaa aagaaatcgc aacttatgcg aaggcaacag ctgaaggtct agctttaact
     4681 tcctacgttt ttgacgagct aaaaaccagt ggtaaaaaag aacctaaaga aaatgatctt
     4741 accctccatg tcgtcgcaaa agattctgac aaagctttca aggccgcttt ctctgagggt
     4801 gtgactctcg gcacttgcgt aaacttctcg cgtcgtctgg gagatttgcc gggcaatctg
     4861 atgactccca caaacttagc ggattctgcc gttgaagcag caaaaggttc tggattgaaa
     4921 gtcactgtct gggacaaagc acgcatcaaa aaagaaaaaa tgggcggcct gctgggggtt
     4981 tccaatggat cttcggaaga gccgcgattt atcatcatgg aatacaacgg gactgcaaaa
     5041 accaaaaagc cggttgtctt cgtaggtaaa ggtcttactt ttgactgcgg tggcatcagc
     5101 attaaacctt ccgccggtat ggaagaaatg aaatacgaca tgtgcggagg agccaacgtc
     5161 attgggaccc tactggctat cgctcggttg aagctcaagg tgaacgttat aggcctagtt
     5221 gcttccaccg aaaaccttgt gggtccaggg gcaacaaaac caggagacgt gcacacggct
     5281 cggaatggaa aaacttttga agtgaacaat accgatgctg aaggccgttt gattttagcg
     5341 gatgctttaa gctacgccac agagcttgaa ccacagatca ttattgatgc ggccactctc
     5401 accggcgcca tggttgttgc cttgggcaat atccatacag gatatttcac tcgcaataac
     5461 gtgttaaaag gcaaagtgga aaaagcagcc gatgcttccg gcgaactcgt ctggaacatg
     5521 ccattaacgg accatcacgt aaaagacatg aaaggcacct tcgcggatct ttcaaatatg
     5581 tcttccggaa aaggcgccgg cagtgccaca gcggccgcat tcctcgagca atttgttggt
     5641 gaaggaattc catgggctca tttcgacatc gcaggaacag gttgggcggt tggaaaccgt
     5701 cttaactatt gtccgaaaaa aggtgctagc ggagccatga tccgtacttt tatcgagatc
     5761 gctaaacagt acgtttaatg gcttctttta tgcccaatta ggctttacaa ttgggtcatt
     5821 ttatgaccca attaaggaga attaatgggt catgctgtgg cccatttgga ggagcataag
     5881 tgggtcccat atacgtgtgg caaaaatcag attggccgaa cttcatctgg gatagtgaac
     5941 agttgttaga agatttaggc caagcacgaa aactacaagg gaaaatcctc ggatttgcta
     6001 atttcgttgg gcttgaagct caggcaaacc tgctggccga agaggccatg acaacttcgg
     6061 caattgaagg tgaaaagctg aacaaagact ccgtgcgatc gtccgttgct cggcgactcg
     6121 gtctctcgac atcaggcctt cctcctcaac aacgagaaat tgatgggctc attgaaatgc
     6181 tgatggatgc tacaagtaat catcaatcac gtctcacggc caagcgactt tttggctggc
     6241 aagcggccct gttccccact ggattttctg gtctgcgcaa aattgcagta ggaacctgga
     6301 gaaaaggtac gcaaccaatg caggttattt caggaagatt aggaaaagaa aaaatacact
     6361 ttgaagctcc gccatcttcc caggttgcta aggaaatgaa agcctttttt gattggtggg
     6421 agggaccatc aaaaaaactc gatggaatta ttcgtgctgg aatcgctcat ttctggtttg
     6481 tgactattca tcccttcgaa gatggcaacg gacgaattgc gcgagcgatc actgatatgg
     6541 cattggccca ggacgaacaa actggttgcc gtttttatag tttatcttct cagatcgtat
     6601 ttgagcgtaa tgcctattac gatatactag aagctagtca gaaaaaaaac tctgatctaa
     6661 ctctgtggct aaaatggttt ttgaaaatgt tttgcctagc catagaaaaa tccgaagggg
     6721 tcgttgccaa ggctttaaat atcgccaggt tctggcaact gcacaatcaa gcggaattta
     6781 acagtcgcca aataaaaata attaatcgtc tattggaagc ggagccagag ggcttcgaag
     6841 gtggaatgac caatcgtaaa tatgtgggac tcactaaagt aagccgcgaa acggccaagc
     6901 gtgatatcgc ggaattggaa gaaaagggag ttcttaaacg caactctggc aaaggccgct
     6961 cgataagcta ttcactatca ttttgagaga gctttttaat gtctgaagct agacactgtt
     7021 ttatttaaaa tataccatca taggacgtcc taggatttca ctcacttcat cagaattaat
     7081 caaaagctct cgttgctcac tggaattcga aagtctcacc gaggttaaaa gccagcgcgt
     7141 tggccactcc gacggcattc catggacccg aatagtgact ggttttcctt cgcgatattg
     7201 aaactctccg tcagcaggtt tttctgctag aactttgtcg acaggaataa aaatgggact
     7261 gatcttggtc atatcctcag aaaattcgca ggccccctct acttccattg ctgggcgctc
     7321 gccgccaata gccatgcctt ctgcttcaaa tctgaaaatg actttttgga actcttggca
     7381 agcaagagtt ttctctcccg tgatctttgc catggcaaag tgaccgagct caaccccgag
     7441 actttgattt ttacgaatca ccgtagagcc agaaataatt ctttttttca tggcatcggt
     7501 aaggtcagtg ccgcttaagt ttgaaaaatc ataaatttgg cgaaccgccg ctggatcacg
     7561 attgatgggc ccccaatttg aagtgtgcag cataattccg taccctacac cccaagagga
     7621 caaaaaaagg agaaaagtca tccctaactt tttcaaaaga cccccaataa tcataagtgt
     7681 ctacatgact catattacag catgataggt cgtatttata tt
//
LOCUS       DATBTE010000050         6191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_141816, whole genome shotgun sequence.
ACCESSION   DATBTE010000050 DATBTE010000000
VERSION     DATBTE010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141816"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VIG33_02155"
     CDS             complement(<1..769)
                     /locus_tag="VIG33_02155"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY8269663.1"
                     /translation="MSVIRYRPEIDGLRAIAVISVLFYHVGFETFRGGFVGVDIFFVI
                     SGFLITRLIRDEVNSSQAFSFSNFYLRRVRRLVPALLFTLGLSSVCAYVLLSPQHLQI
                     FGGSLASAITSLSNFYFWRESGYFDAEVKFKPLLHTWSLCIEEQFYLFWPLLVTFLLL
                     KKDKRYLLGTSIALFSISLLANLVITEKFPDSASTIYYLAPFRVFEFMVGAVLVELVQ
                     FQTKKQWVHEVLIVLGLIMILFPALVFTEKTVFPSYNA"
     gene            1227..2258
                     /gene="rfbB"
                     /locus_tag="VIG33_02160"
     CDS             1227..2258
                     /gene="rfbB"
                     /locus_tag="VIG33_02160"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEY8269664.1"
                     /translation="MNILVTGAAGFIGSSFVRKALRDKNTVIVLDKLTYAGHIENIEE
                     LIDNSLCHFVQGCITDQGLTEKLLKEHQIDYLVNFAAESHVDNSIHSPGEFIKTNIFG
                     TFSLLEAARGYWGALTGSKKDHFRFLHVSTDEVYGTLGDTGKFNEELPYRPNSPYSAS
                     KAGSDHLVRAWHHTYKLPTITTNCSNNYGPRQYPEKLIPTIITNALNGKHLPVYGKGQ
                     NIRDWIHVEDHAHGVYLALTKGTSGETYCFGGNSERTNLDVVNSICAILDEVRPRADK
                     QSYHQQIKFVEDRAGHDWRYAIDDTKAQKEIGFTRTYTQFEQGLRATVEWYLANNVWI
                     EKVTAKNKK"
     gene            2269..3150
                     /gene="rfbA"
                     /locus_tag="VIG33_02165"
     CDS             2269..3150
                     /gene="rfbA"
                     /locus_tag="VIG33_02165"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEY8269665.1"
                     /translation="MKGILLAGGNGTRLFPTTKSVSKQLLPVYDKPTIYYPLSTLMLA
                     GIREILVISTPRDTPLIQELLGDGSSLGMQISYKVQPKPEGIAQAFLLAEEFLQGSPT
                     CLILGDNLFYGHDMTTQLTNCSRLKQGATVFAYHVQDPERYGVVEFDDGTAKALSIEE
                     KPKQPKSNWAVTGLYFYDGSVVERTKKLKPSPRGELEITDLNKSYLQEGFLKVEKLGR
                     GVAWLDTGTHESLISSSQFVQTIEARQGLKIACIEEIAYFKGFIDHQQLEKLVESYPT
                     SSIYTEYLKKLLAGKLN"
     gene            3159..3701
                     /gene="rfbC"
                     /locus_tag="VIG33_02170"
     CDS             3159..3701
                     /gene="rfbC"
                     /locus_tag="VIG33_02170"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEY8269666.1"
                     /translation="MEIQKFDIQGPLLIHLKTFHDNRGFFVERYNENKFKELGLPTNF
                     VQDNFSRSAYGVLRGLHYQNTPPQGKMVSCTFGEIFDVAVDVRKDSPTFGKHVSVRLQ
                     GDHPALFWVPAGFAHGFCVTSKQGADVMYKVDNLWSAAGEGSILWSDPDLQIPWPLET
                     PILSEKDAKCPLLKNRGRIF"
     gene            3876..4526
                     /locus_tag="VIG33_02175"
     CDS             3876..4526
                     /locus_tag="VIG33_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269667.1"
                     /translation="MSWKFHQKTGLSGQEFLAHISKNPGADVYFVNPFPELCIYRSVW
                     EHGQEFHPNLKKLTVKLFKECGYSDQILKKETPPNLTAYCNHWVANKQFWDSYIEFLT
                     PFWKYVRKDKGPLCLSLKKMADPIINAPYLPFIFERLFSTFLAEHKFKVSSLPIPPSK
                     ILDNPYLSPVSNRIIRVRDLNNHKLVLRTDRFAIGFFSIIRKFRHYYLPLIIKKIR"
     gene            complement(4555..5331)
                     /locus_tag="VIG33_02180"
     CDS             complement(4555..5331)
                     /locus_tag="VIG33_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269668.1"
                     /translation="MGNRSIYYLYIQFGDSKQKEGLARIEKFHRKNFNKFNMRVLIVD
                     NKYDRKNIFIEYTNDIVKIAGDNENREFSAYDLAVRSAKHLFNVHEEDVVIIANDTFF
                     THYGDEYLEKFNKKRIRACIKENAILGHIDKFPKDVTLFDLSFQEWIRTSFFLTKFSV
                     LNKIGPLAIPWNDQLLFSGVTKPFFSPQAALSENYKKYLSYWLFDFENLVSDFPYEWH
                     SKKPLNEQNVGEFRGKVRSILAEHYFSARAQRLGIPLLSL"
     gene            complement(5324..>6191)
                     /locus_tag="VIG33_02185"
     CDS             complement(5324..>6191)
                     /locus_tag="VIG33_02185"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8269669.1"
                     /translation="LWPQSVPLMKISKAQGAKVFFEFVECVSRSVWIDIQRKSTIEDV
                     EPLVQKFVSCLVTECAAANMADGCIALTPEDSSFVTQIMRPKSIDIFPTGISQFAVLD
                     KLKEYRAGLAESEKSAIFVGYYDHTPNIEGLDWYFKEVHLKIIAAIPEFKVKIVGRGS
                     LTPLKEKYRSLPGIEWIGEVADIVPHILSSYICISPLISGAGLRGKIIQYSMLCRPTV
                     STPIGVCGTPFVDNKSVVIADTPEAFARGVIKLIGDNDFYEKIAASAKAEAEVHFSWA
                     PVIEKMEIFLRG"
BASE COUNT         1875 a         1329 c         1263 g         1724 t
ORIGIN      
        1 gggcattata cgaaggaaaa acagtttttt ctgtaaacac cagtgcggga aataaaatca
       61 ttatcagccc taaaacaata agaacttcat gaacccattg ttttttggtc tgaaactgga
      121 caagttccac aagaaccgca cctaccataa attcaaatac tcgaaatggc gcaagataat
      181 aaatcgttga tgcgctatcg ggaaattttt cagtgataac cagatttgct aaaagggata
      241 tggaaaacaa agctatgctt gtgccgagga gataacgttt atcttttttc aggagtagga
      301 atgttactag caaaggccag aataaataaa actgttcctc aatacataag gaccaggtat
      361 gaagcaaggg cttaaatttt acttcagcgt caaagtaccc actctctcgc caaaaataaa
      421 aattcgacaa gctggtgatg gcggaggcaa gggagccgcc aaaaatctgg agatgctgag
      481 gtgacaataa aacataggcg caaactgagc tgagccccaa ggtaaagaga agggccggga
      541 ccaaacgtct cacgcgacgt aaatagaaat tagaaaaact gaaggcttga gaggagttca
      601 cctcatctct tataagtctt gtgattaaaa aaccgctgat cacaaagaaa atatcgaccc
      661 caacaaagcc accacgaaaa gtctcaaaac caacatggta aaataacaca ctgataacag
      721 ctattgccct caggccatct atttcaggtc tatatcgaat gacagacatc tctccaccaa
      781 ggaaattaaa aagatatatt aataaaccat ttgtttatag cacagtttgt ggcttaacca
      841 ttgattttgt caccatagat ttacttacat cttcgcaaaa ccaaaggaat caagctctcc
      901 gcacctatct agttggaatt gatttcctca gaccaacaat acactaacaa agggtctctg
      961 gttaaaaagc agtaccaaac aaagaaaaat atctttcagt attctataaa ataatcagta
     1021 aggacaattt cgcctcgtgt cgaatctttg ctgacgtaaa cctctcgctt gtctaaattg
     1081 ccacacccaa tagccgctca gataaaaact caagactgta aataatgaaa gaaataaacg
     1141 atttcgcagt tatccggtcg tgacaagtct tgactcgttt tttataaaag accactctag
     1201 taaagacatt ttatcagggg tgggatttga atattttagt aaccggggcc gccggattta
     1261 ttggcagttc ctttgtcaga aaagctctca gagacaaaaa cacagttatt gttttagata
     1321 aacttaccta tgcgggccac attgaaaata ttgaagaact tatcgataac agtctctgcc
     1381 acttcgttca aggttgcatc actgatcagg gcttgactga aaaacttctt aaagagcatc
     1441 agattgatta tctggttaat tttgcagctg agtcccacgt ggacaactcc attcactccc
     1501 cgggtgagtt tattaaaaca aatatttttg gaactttctc acttcttgaa gcggctcgcg
     1561 gttattgggg tgcgctgacg ggttccaaaa aagatcattt ccggttcttg cacgtttcta
     1621 cagatgaagt ttatggaact cttggtgata cggggaaatt caatgaggag cttccttacc
     1681 gtcccaactc tccctactct gccagcaagg ccggaagtga ccacttggtc agggcttggc
     1741 accatactta caagctcccc accattacca caaactgttc caacaactac ggtcctcgcc
     1801 aatatccgga gaaactcatc ccaacgatca tcaccaatgc actgaacgga aaacaccttc
     1861 ccgtctatgg gaaaggacaa aatatccgtg attggatcca cgttgaggat catgctcacg
     1921 gtgtgtatct cgccttgacc aaggggacct cgggtgaaac ctattgcttc ggcggaaatt
     1981 ccgaacgcac caatcttgac gtggttaact ctatttgtgc tattctggac gaggttcgcc
     2041 cccgcgctga caaacagagc tatcatcagc aaataaaatt cgtagaagat cgtgcgggcc
     2101 acgattggcg ttatgcgatt gacgacacga aggcacaaaa agaaattgga ttcacaagaa
     2161 cttacaccca attcgagcaa ggcttgcgcg ccactgttga atggtatctg gcaaataatg
     2221 tttggattga aaaagtaact gcaaagaata aaaagtaaga ggtcggtaat gaagggtatt
     2281 ttattagctg gcggcaacgg cactcgactt tttcccacga caaagtctgt atcgaaacaa
     2341 cttttgccgg tttatgataa gccgacgatt tactatccgc tttcgacttt gatgctcgcc
     2401 gggatccgtg agattttagt cattagcacg ccacgggaca ccccacttat ccaagagttg
     2461 cttggggacg gcagcagctt gggaatgcag atttcttata aagtgcagcc gaagcctgaa
     2521 ggaattgcgc aggcgttcct attggccgag gagttccttc aaggttcgcc gacttgcttg
     2581 atcttgggcg ataatctttt ttacggacat gatatgacga ctcagctcac gaactgttct
     2641 aggctcaagc agggcgccac cgtctttgcg tatcatgttc aagatcctga acgctatggg
     2701 gtggttgagt ttgacgatgg cacagccaaa gccctgtcta tcgaggaaaa accgaaacaa
     2761 ccaaaatcaa attgggctgt tacaggctta tatttttatg atggcagcgt tgttgaaaga
     2821 actaaaaagc taaagccttc tccgcgcggc gaacttgaga ttacggatct gaataaaagc
     2881 tacctccaag agggcttcct aaaagttgaa aaactgggac gtggtgttgc ctggctcgat
     2941 accggaactc atgagtcttt aatttcgtct tcccagtttg ttcagacgat tgaagcacgt
     3001 caaggtttga aaattgcttg tattgaggaa attgcttact ttaaagggtt tattgatcat
     3061 caacagcttg agaagctggt ggagtcttat ccaacgagct caatttatac ggagtattta
     3121 aagaagcttc ttgctggtaa attgaattag gagttgtcat ggaaatacag aaattcgata
     3181 ttcaagggcc cttgctgatt cacttaaaaa catttcatga caacagaggt ttttttgttg
     3241 agcgctataa tgagaataag tttaaggaac tgggtcttcc tacgaatttt gttcaagata
     3301 atttttctcg gtcagcctat ggggttttaa gagggcttca ttatcaaaac acgcctccgc
     3361 agggaaaaat ggtctcttgc acctttggcg agatcttcga cgttgccgtg gatgtgagaa
     3421 aggattcgcc tacatttggc aaacatgtgt ctgtcaggct tcagggggat catccggctt
     3481 tgttctgggt ccctgcgggc tttgctcacg ggttttgcgt gaccagcaaa caaggagctg
     3541 atgttatgta taaagtggat aatttatggt ccgcagccgg agaggggtcc attttgtgga
     3601 gtgatcctga tctgcaaatt ccatggccac ttgaaacccc cattttgagc gagaaagacg
     3661 caaagtgccc cctcctcaaa aaccgtggcc gtatttttta aatgaagacc aaaatatatc
     3721 aaatatttta ccgcaactcg cagaaggatt ctttagatcc ggatcttatt ccgtataata
     3781 actagggcaa gagttatccg tataattttg aatatgcagt tttttttgat ctttatgaaa
     3841 agacgaattg gaataatacg gatttactag gatcggtgtc gtggaaattt catcaaaaga
     3901 cgggcctgag tgggcaagag tttttagctc atatttctaa aaatccaggg gcggacgtct
     3961 attttgtgaa tccttttccg gaactttgca tttaccgaag cgtttgggaa catggacagg
     4021 aattccatcc gaatttaaaa aagctaacag tgaagctttt taaagaatgt gggtattctg
     4081 atcagatttt aaaaaaagaa accccgccta atttaaccgc ctattgtaat cattgggtgg
     4141 ccaataagca gttttgggat tcttatatag agtttttaac tcccttttgg aagtatgtaa
     4201 ggaaggacaa gggccccctc tgtctttccc tgaaaaaaat ggcagatccg atcatcaatg
     4261 ccccttatct cccgtttatt tttgaacggc tcttttctac ttttttagct gaacataagt
     4321 tcaaggtctc atctcttcct atccccccca gtaaaatcct cgacaatccc tatctatcgc
     4381 cagtttcaaa caggattatt agagtgagag acctcaacaa ccataaatta gtcctaagga
     4441 ccgatagatt tgcaattggt tttttctcta taattaggaa atttcgccac tattatttgc
     4501 ccctcatcat aaagaaaatt cgttagttaa aactgagagc ctcagcactc tcacttagag
     4561 tgacaacagc ggaattccca gacgttgagc ccgggcacta aagtaatgtt cggctagtat
     4621 cgaccttacc ttgcctctga actccccgac attttgttca ttcaaaggtt ttttcgaatg
     4681 ccattcataa ggaaaatcac tgaccaggtt ttcaaagtca aaaagccaat agctgagata
     4741 ttttttatag ttctcactca aagccgcctg tggggaaaaa aaaggttttg tgacgccact
     4801 aaatagaagt tgatcgttcc aggggatcgc gaggggacca attttattta gcacagagaa
     4861 tttggttaaa aaaaatgaag tcctaatcca ctcttgaaag ctcaagtcaa acaaggttac
     4921 atctttagga aatttatcaa tgtgacctaa aatggcattc tcttttatgc aggccctgat
     4981 tcttttttta ttaaatttct ctaagtattc atcgccatag tgggtaaaaa aagtatcatt
     5041 cgcgataatg acaacgtctt cttcatgaac attaaacaag tgttttgcag acctcacagc
     5101 caaatcataa gccgaaaact cacggttctc attgtcgccg gcaattttga cgatatcatt
     5161 cgtatattca ataaaaatat ttttacgatc atacttatta tcaacaatga gaaccctcat
     5221 attaaattta ttaaaatttt tgcgatgaaa tttttcaatt ctagcaagcc cctctttctg
     5281 cttcgaatca ccaaactgaa tatagaggta gtaaatagac ctgttaccca cgtaaaaaaa
     5341 tctccatttt ttctatgaca ggagcccatg aaaagtgaac ttcggcctcg gctttcgcac
     5401 tggccgcgat tttttcataa aaatcattat cacctatgag tttaatcact ccacgggcaa
     5461 aggcttctgg ggtgtccgca ataaccactg atttattatc tacaaaggga gtcccacaca
     5521 ccccaatagg agtgctgacg gtcgggcggc ataacattga gtactggata attttccctc
     5581 tgagacctgc accagaaata agaggggata tacagatata cgaagacaaa atatgcggga
     5641 caatatctgc cacctctcca atccactcaa tcccaggcaa tgaacgatac ttttctttca
     5701 agggagttaa gctgcctctt cccacgattt taactttgaa ttctggaatc gctgctataa
     5761 ttttcaaatg gacttccttg aaataccagt ctagtccctc gatattggga gtatgatcgt
     5821 aataccccac aaaaatggca gatttttcag actctgccag gccagcacga tattctttaa
     5881 gcttatctaa gaccgcaaat tgagagatgc ctgtgggaaa aatatcaatg gatttcgggc
     5941 gcataatttg cgtcacaaaa cttgagtctt cgggtgtcaa agcaatacat ccatccgcca
     6001 tatttgcggc tgcacactcc gtcaccaaac aggaaacaaa cttttgcact agaggctcga
     6061 catcctctat ggttgatttt ctttggatat caatccatac gcttctcgac acacactcga
     6121 caaattcaaa aaatacctta gccccctgtg ctttgctgat tttcattaag gggactgatt
     6181 gaggccacaa g
//
LOCUS       DATBTE010000051         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_5538, whole genome shotgun sequence.
ACCESSION   DATBTE010000051 DATBTE010000000
VERSION     DATBTE010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5538"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VIG33_02190"
     CDS             <1..227
                     /locus_tag="VIG33_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269670.1"
                     /translation="VVKDSLQVAFNGFADSTLNILVNFHLAVFDANEELARQQKIFCE
                     IIEAAKEMGVVFAYPTQTVYYMSPQEPAQK"
     gene            complement(277..723)
                     /locus_tag="VIG33_02195"
     CDS             complement(277..723)
                     /locus_tag="VIG33_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269671.1"
                     /translation="MSCHNLNSCYALGLADSLWQISPTNMADIYYSSGNVGIGTTLPG
                     QRLSVAGMIESTSGGLKYPDGTTQTTSTIAVIKVDGLSPSAPCNGSFTTVAICPAGYT
                     VSGCSAWTGNFANPAGAWKSGNGCQGGCGGGGNNDIGVQAICIKLQ"
     gene            complement(765..1355)
                     /locus_tag="VIG33_02200"
     CDS             complement(765..1355)
                     /locus_tag="VIG33_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269672.1"
                     /translation="MTKKEAKVSAIKSGVYKHSELTEMLGSRMAVKRALAEQLVEKVS
                     RGFYCTPDIATNEAYYRVIKKFYKDAVISKRTLLYHYRLTTNQPDLIDVDVPQTSKLR
                     NGTDLLEVHRTNKIFGLSSAEFNGIKLKCYSVERALFDVLSFEEKPGSLTSEIIHNYL
                     SSFKYEPAVIHALGLRFGSRGAQLANLIQVMAGDKF"
     gene            complement(1380..2150)
                     /locus_tag="VIG33_02205"
     CDS             complement(1380..2150)
                     /locus_tag="VIG33_02205"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEY8269673.1"
                     /translation="MSNFKKNFNKWKTDTGEHGPRALTRYVIMLFVEGLQEVAPDKYI
                     FKGGNLLWHYIKTPRSTVDVDFATEADVEVDEVISDFKRVSAEDCVFKVLDYKVIASD
                     DKSGASVQMEFLTSDGSMNPFGIDIVFAVQTHSKQIKLARSEVKAASLENIIVDKVAA
                     CWRFGGGNTRMKDLDDLYRIAKTQPSIDSKVLVSLARDRQVRLTLDRKWISPPMEGAW
                     KDYIQKKVYKGASDLPSVLGQVFESINKYLNNVTNVAK"
BASE COUNT          714 a          651 c          564 g          723 t
ORIGIN      
        1 ccgtggttaa ggacagtttg caagtcgcgt ttaatggctt cgcggattcg actttgaata
       61 ttttagtgaa ttttcatctg gccgtcttcg atgccaatga agaactcgct cgccagcaaa
      121 aaatcttttg cgaaatcatc gaggcggcaa aagaaatggg agtggtcttc gcttatccaa
      181 cgcagacggt ttactatatg tcgccacagg aaccggccca gaagtaaggg ctatttggag
      241 caaaagctaa ttggaccgat gagatcccct tttgtactat tgaagtttga tgcagatggc
      301 ctgaacgcca atatcgttgt taccgccgcc tccacagccg ccttggcaac cgtttccgga
      361 tttccaagcc ccggctggat tagcgaagtt gcctgtccac gcagaacagc cagatacagt
      421 gtatcccgca ggacagattg caaccgtagt gaagctccca ttgcatggcg cgctcggact
      481 taggccatcc accttgatga ctgcgatggt actggtcgtc tgagtggtcc cgtctggata
      541 ttttaatccg cccgaggtcg actcgatcat tcctgcaact gaaagcctct ggcctggtaa
      601 agtagttcca atgccgacat tgccgctgga ataataaata tccgccatat tcgtgggaga
      661 gatttgccac agtgaatccg caagtcccag ggcataacaa ctattgagat tgtgacagga
      721 cattttgtaa aaaggagcat ctaaagaaat atgcgctttt gaacttaaaa cttatccccc
      781 gccataactt gtatcaaatt cgcaagctga gccccacgag acccaaacct caaacccaga
      841 gcatgaatca ctgctggctc atacttaaaa ctagaaagat agttatgaat aatttcagaa
      901 gtaagactgc cgggcttttc ttcaaaagaa agaacatcaa acaaagcgcg ctcgacagaa
      961 tagcatttta gttttatgcc attgaactcg gcggaactca gaccgaaaat tttattcgtc
     1021 ctatgaacct caagaaggtc tgttccgttt ctcaacttag acgtttgcgg cacatcaaca
     1081 tcaatcaagt ccggctggtt ggtggttaaa cgataatgat acagcagcgt ccgcttggaa
     1141 atgaccgcat ctttatagaa tttcttaatg acccggtaat aggcctcatt ggttgcaata
     1201 tccggtgtgc aatagaagcc tcttgatact ttctccacca actgctccgc gagagccctc
     1261 ttaacggcca tgcgagagcc cagcatttca gtcaactctg agtgcttgta gactcccgat
     1321 ttaatagcag aaactttggc ttcttttttc gtcatgttac tattttagct aaaaatttac
     1381 tatttagcaa cattagtaac attattgagg tatttattaa tagattcgaa tacttgacca
     1441 aggacagatg gcagatcaga agccccttta tagacctttt tctgaatata atccttccag
     1501 gcaccttcca tcggtgggct gatccatttc cgatcaaggg ttagccttac ctgccgatcc
     1561 ctcgccaaac tcactaaaac cttggagtct attgagggct gcgtcttggc tattcgatag
     1621 aggtcatcca agtccttcat gcgagtattc ccgccgccaa agcgccagca cgccgcaact
     1681 ttgtcgacga tgatgttctc aaggctcgcc gctttaactt ctgatcgcgc caacttaatc
     1741 tgcttgctgt gagtttgcac tgcgaaaaca atatcaatgc cgaagggatt cattgaaccg
     1801 tcagaggtca aaaactccat ctgaacggat gctccgcttt tatcatcgga cgctataact
     1861 ttataatcta aaaccttaaa tacacagtct tcagcactga ctcttttaaa gtcagaaatg
     1921 acttcgtcga cttcaacgtc agcttcagtc gcaaaatcca catcaacagt ggatcgcggc
     1981 gtcttgatat aatgccagag aagattgccg cctttaaaaa tgtatttatc cggcgcgact
     2041 tcttgaagtc cctctacgaa aagcattatc acgtaacgag taagggcgcg tggcccatgc
     2101 tcacccgtat cagtcttcca cttattgaaa ttctttttaa aattactcat tttctaattt
     2161 tccttatatc actcattttg ctttccacga actgttgaaa caaagtcgac tctacgagcc
     2221 agatgtaacg cttaccaagt aggccctgga ttcagggtat tttttcacgc agacacactt
     2281 gccgactctc aaggatttaa tgatgtttgg atggatcaca aactcctcca cctcacgaag
     2341 tgacttgttt cccgtgggaa catcaaacaa caacatcttc gccgactgtg gggctaagga
     2401 aaacaatagc ggttttccta ggggcggccc gggtccaagc ggccatgagg ccgctgcatt
     2461 cctgagcttc ttgtaaagat tttatgttac gtgaagccgg tgctgcctcc gaatctctca
     2521 agaagagacc gtggttaagg acagtttgca agtcgcgttt aatggcttcg cggattcgac
     2581 tttgaatatt ttagtgaatt ttcatctggc cgtcttcgat gccaatgaag aactcgctcg
     2641 ccagcaaaaa at
//
LOCUS       DATBTE010000052         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1303337, whole genome shotgun sequence.
ACCESSION   DATBTE010000052 DATBTE010000000
VERSION     DATBTE010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303337"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VIG33_02210"
     CDS             complement(<1..193)
                     /locus_tag="VIG33_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269674.1"
                     /translation="MKKLLIGLSVFVLSFSAVATSLKTQKLVATVSNYSCEEILNILL
                     EKNAEVSRLVAVGFDAAAEE"
     gene            complement(471..>1556)
                     /gene="nagZ"
                     /locus_tag="VIG33_02215"
     CDS             complement(471..>1556)
                     /gene="nagZ"
                     /locus_tag="VIG33_02215"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY8269675.1"
                     /translation="HLIIGISGTALTADEKKFIINNNIGGIVLFDRNISDPRQVHALC
                     SEIQSLRHEMIEKAPLFIGIDMEGGRVARLKAPFTVWPPLKSLGDLDNATVTFHFANR
                     MGLELHAVGINLDFAPCVDVFTNPKNTVIGDRSISSDAEIVARHATALVRGFIKSNII
                     SCAKHFPGHGHTLVDSHEDLPLEELDLQRLDSLELIPFKRSFKARVELVMTSHILFKN
                     IDPEWPVTLSEKFLKKILREDYRYRGLIVTDDLDMKALTKHYDSKQIPVKALQAGADL
                     LLYCNEPLSPPRAIEAVTEALTQNQLKKADLEASYKRILELKKDKIPHPDPMPLEEAI
                     KIIGNPEHQNLAQAIVRGEVPAGLIEV"
BASE COUNT          440 a          309 c          346 g          461 t
ORIGIN      
        1 tttcctcggc agctgcatca aatccaaccg ctaccagccg cgatacttct gcgtttttct
       61 ccaaaagaat attgaggatt tcttcgcatg aatagttact tactgttgcg acgagtttct
      121 gtgttttcaa tgaagtagcc acagcagaaa aagacaaaac gaaaacagat aatcctatga
      181 gcaatttttt catattctct ccaaaaggca gttagagagc gttctaaatt tataagagaa
      241 ctattctcct aaggtcagat cgctttatta tgggggtttg taaagcaaag atcctgccaa
      301 tattttcggt ggtataaatt gatttattag acttaaatca aaactctgtg aaaactgaag
      361 taatttcttc aatatttggc gttgaacttt taaacattga gaattttaaa tgagtataca
      421 gcccgcaact tatctttagt tgtgaactgt ctgggcgaac gataaattgt ttaaacttca
      481 ataagtccgg cgggaacttc gcctcttaca attgcttgtg ccagattttg atgctcggga
      541 ttgccgataa tttttatcgc ttcctctaac ggcattggat ctggatgtgg aatcttatcc
      601 ttctttaact ctaagatacg cttatagctg gcttctaagt cagccttttt caattgattt
      661 tgagtaagag cctcagtcac tgcctcaatg gcgcggggag gggaaagggg ttcattgcaa
      721 taaagaagaa ggtccgcgcc cgcttgcagc gcttttacgg ggatttgttt agaatcataa
      781 tgtttcgtca acgctttcat gtcgaggtcg tcggtgacaa tgagtccgcg atagcggtaa
      841 tcttcgcgaa gaattttctt taagaatttt tctgataaag tcacaggcca ttcaggatca
      901 atatttttaa ataatatgtg cgaagtcata actaattcaa cgcgggcttt gaaggaacgt
      961 ttgaaaggaa taagctcgag gctgtcgagt ctttgcagat ccaactcttc aaggggtaaa
     1021 tcctcatggc tatctacaag ggtatggcca tggccaggaa agtgcttcgc gcaagaaatg
     1081 atatttgatt taataaagcc tcgaaccaga gccgtggcat gtctggctac gatttcagca
     1141 tcactgctaa tggagcgatc accaatcacc gtattttttg gattggtgaa gacatccaca
     1201 caaggggcga aatctaggtt gatgccaacc gcatgaagct caaggcccat cctattagcg
     1261 aagtgaaaag ttacggtcgc attatcgaga tcgcccaagg atttaagggg gggccaaact
     1321 gtaaaaggcg ctttgagtct tgcaactcgt cctccctcca tatcgatacc aataaaaagc
     1381 ggagcctttt ctatcatttc gtggcgaagt gattgaattt cagaacagag ggcatgtact
     1441 tgcctcggat cagaaatatt tctgtcaaag agcacgatgc cgccaatgtt attattgatg
     1501 ataaatttct tttcgtcggc cgtgagagca gtgccggaaa ttccgatgat taagtg
//
LOCUS       DATBTE010000053         2013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_5623, whole genome shotgun sequence.
ACCESSION   DATBTE010000053 DATBTE010000000
VERSION     DATBTE010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5623"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..1025
                     /locus_tag="VIG33_02220"
     CDS             237..1025
                     /locus_tag="VIG33_02220"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY8269676.1"
                     /translation="MTLIQALILAVVEGLTEFLPISSTGHMVLTSYLMGIEENTFVKS
                     FEVIIQFGAILAVVVIYWKRFLPHWSFYKKLFVAFLPTGIIGFLLKNYIDILLSSPSV
                     VAWSFIIGGLILIFTDKYFAHLNEEGRKIDQLQYKDSLLLGLFQSIAMIPGVSRSAAT
                     IIGGLSLGMNRKDAAEFSFFLAVPTLAAATLYKSFKLFKTIESSQIHLLLIGNIVAFV
                     VAMLAIKSFIKFLSSHGFKYFGYYRIIFGLLFLIMIYSGAELKL"
     gene            1022..>2013
                     /locus_tag="VIG33_02225"
     CDS             1022..>2013
                     /locus_tag="VIG33_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY8269677.1"
                     /translation="MNDGSHWLNQIFFLIPTWKWLTLLTVFAVGVVFFSSFRYAARKL
                     KNLHFFKSKPTGFWHYYSQMPIENALAWIIITLIWFFTLESLDLTPRLDKYLTVVVKI
                     IFSLNALRLSYIAMEALGEVVKAYTAKTPSALADHLAPMAVKSGKVFVVILGALIVLQ
                     NFGVNVMSLLAGLGLGGLALALAAQDAAANLIGSVMILIDRPFSIGDAVKVNDVEGFV
                     ESLGFRSTRIRTFYNSLVTIPNSTMAKEKIDNMGLRPYRRVRQILGLHYDTPPELIEQ
                     FCVRLKYLISQEETVVKDSLQVAFNGFADSTLNILVNFHLAVFDANEELARQQK"
BASE COUNT          497 a          444 c          425 g          647 t
ORIGIN      
        1 taatgttgag aaagcgcgtg tgtttctgta tttcaccaag tattcccccg cgaatacttg
       61 agaagaaacg agcaacgcgc ttactaatag aatgcgtttc ataatttacc tccgtgtaaa
      121 aaaatcatag tcagatgtgg gcaacaaaat atagaaaaat cttttaagaa tcttaatttt
      181 atgacacgca aagcaacagt tgctcactga gggaagctct tgaacaatct tttcctatga
      241 cattaataca agctctcatt ctcgcagttg ttgaaggatt aacagaattt ttgcctatct
      301 cttccaccgg acatatggtt ttgacatctt atttgatggg catcgaagaa aatacatttg
      361 ttaaaagctt cgaagttatc attcagttcg gagccattct ggcggttgtt gtcatttact
      421 ggaaacgctt tttacctcat tggagtttct acaaaaaact cttcgtcgct ttcctgccaa
      481 caggcattat tggattttta cttaaaaact atatcgatat tcttttaagc agtccgagtg
      541 tggttgcttg gtcttttatt attggcggcc tgatccttat cttcactgac aaatacttcg
      601 cgcaccttaa tgaggaaggc cgaaaaatag atcagctcca atataaagac agccttttgc
      661 tagggctctt tcaatccatt gccatgattc caggagtgtc tcggtccgcc gccacaatta
      721 taggaggcct cagcctcgga atgaaccgca aagacgcggc agaattttct ttcttcttag
      781 cggtccccac gttagccgcc gccactcttt ataaaagctt taaacttttt aaaaccattg
      841 aatcctcgca aatccatctg ctcctcattg ggaatattgt ggcctttgtc gtggccatgt
      901 tagctataaa atcttttatt aaatttcttt caagtcatgg ctttaaatat ttcggttact
      961 accgtattat cttcggtctt ctctttttaa ttatgattta ctcaggtgcg gagttaaaac
     1021 tatgaacgat ggatctcact ggctgaacca aatatttttt cttattccaa cctggaaatg
     1081 gctgaccctg ttaacggtct tcgccgtcgg cgtcgtcttt ttttcaagtt ttcgctatgc
     1141 ggcccgcaaa ttaaaaaatc tgcacttctt taaatcaaag cccacgggct tttggcatta
     1201 ttactctcag atgccgattg aaaatgctct ggcctggatc attatcacct tgatatggtt
     1261 cttcactctg gagtctttgg atctcactcc ccgtcttgat aagtacctga cggttgtagt
     1321 gaaaatcatt ttctccttaa atgcccttcg tttaagttat atcgcgatgg aggctttggg
     1381 tgaagtcgtc aaggcttaca ccgccaagac accgagtgct ttggcggatc acttggctcc
     1441 catggcggtg aagtcgggaa aagtgttcgt ggtgattcta ggagccttga ttgttttgca
     1501 aaacttcggt gtcaatgtca tgtccttgct ggcgggtttg gggttgggag gtttggccct
     1561 agccttagct gcccaagatg ccgccgccaa tctaattggt tcggtgatga tcctgatcga
     1621 tcggcccttt tccattggcg acgcggtgaa ggtgaatgat gtggagggtt ttgtcgagtc
     1681 tctgggcttt cgttccactc gtattcgcac cttttacaat tctttggtga ccattcctaa
     1741 ctccaccatg gctaaagaaa aaatcgacaa tatgggattg cgtccttatc gccgtgtgcg
     1801 ccagatcttg gggcttcatt acgacactcc gcctgaattg attgagcaat tctgcgtgcg
     1861 tctgaaatat ttaatctctc aagaagagac cgtggttaag gacagtttgc aagtcgcgtt
     1921 taatggcttc gcggattcga ctttgaatat tttagtgaat tttcatctgg ccgtcttcga
     1981 tgccaatgaa gaactcgctc gccagcaaaa aat
//
LOCUS       DATBTE010000054         3377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_74150, whole genome shotgun sequence.
ACCESSION   DATBTE010000054 DATBTE010000000
VERSION     DATBTE010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74150"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1447
                     /locus_tag="VIG33_02230"
     CDS             200..1447
                     /locus_tag="VIG33_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269678.1"
                     /translation="MDSRIHSKASFYLMAVVALVVLSACGEKKSARGSQPIEVPKTNT
                     TGSDQPLPAPSGGGVELPLPQAPGPNPPGPLKDVLDEDSDYDPVDPKAVEKADFQKRY
                     TGMGDDEGLLYTGSSTDYILTYLRLRNEDPNLDADVKARNLEAAQSIESARLSHDKIT
                     GDLLVSLKVREGNNLKSYVLTGGLSDGDTLSSLRLAKGSGGGRGKTTGAQHIEGKIRC
                     MDSDGGCDTTVARLVIGDHPGTRAAVAIVFRESLADVYFSLSAATGNPEYDVMRKFWI
                     HSNDEANTDLRLKEAVLKSFEIVNGRSGFDVQIKSYGQQLLSFGGPLLAPDAGTSVSM
                     RANRNAELKESLDLDGLTDYKFDLANFIGSAKIVNNNGLGQVRLAVKMRKRVGYRQES
                     FSLTLMRIIKPTLNPNQENLKLN"
     gene            1572..2531
                     /locus_tag="VIG33_02235"
     CDS             1572..2531
                     /locus_tag="VIG33_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HEY8269679.1"
                     /translation="MHGTSFEFAKTTTNSNTSISAPHVSDYMDYRQYLASFFEYKRKQ
                     TASDLRPYNYAAFSAGANIKSPNYLKMIIEGRRNLSGEMVLKFAKALGLNKEATEDFN
                     LLVSYGQATDPANRNMFLKELSEHRVQVKLKSGEIDQKTWDKIPNWAAWVIYAMVDQE
                     GVSYDTKTLRELLRGKASEDEIEQALESLFKSGELAQDPETGKVRKAHTLIEAAEDIP
                     VALVRKLQAQLMYLGLESLYQDAATEREFGTLTMSLTKQEFEEIKFKLRQIRKQINKD
                     NSIARASSKGEKVYQLNIQLFPVTNATNKSAHVEKVSKEDASH"
     gene            2815..3168
                     /gene="ssrA"
                     /locus_tag="VIG33_02240"
     tmRNA           2815..3168
                     /gene="ssrA"
                     /locus_tag="VIG33_02240"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          948 a          686 c          841 g          902 t
ORIGIN      
        1 tttcaaaaat taaattctcg cttttcgaaa gtacctggtg tcatttttga tgacagtttt
       61 ttttacgctc gtgaagtttt ttccttctta attctctgat tttctctcta ggagcgttga
      121 atttgagtac agggatgaca atttttggcc cattgcttgc tctttctcga gcgaacgtaa
      181 aaactcggag gtcttcatta tggattctag aatccactca aaagcttctt tttacttaat
      241 ggcggtggtg gcacttgttg ttctctcggc ctgtggtgaa aagaaaagtg cacggggctc
      301 gcagcctata gaggtaccaa aaactaatac gacaggcagt gatcaaccgt tgccagcacc
      361 cagtggcggc ggagttgaat tgccattgcc tcaagctcca ggcccaaacc ctccaggtcc
      421 gctgaaagat gttctggatg aggattcaga ctatgatccg gtggatccta aggcggtgga
      481 gaaagctgat ttccaaaaac gctacaccgg catgggtgat gatgagggcc ttctttatac
      541 gggctcttcg actgattata tcttaacgta tttacgtctt cgcaatgaag acccgaatct
      601 ggatgcggac gtcaaagcac ggaatcttga agcggcacaa agcattgaat cggcccgctt
      661 gagtcatgat aaaataactg gagatctttt ggtttcttta aaagttcgtg aaggcaacaa
      721 tcttaagagt tacgtgctga cgggcggttt gagcgatgga gacacgctct cttctttgcg
      781 tttagcaaaa ggcagtggtg gtggccgagg aaaaactacg ggagctcaac acatcgaagg
      841 taaaatccga tgtatggatt cggatggtgg ttgtgatacc accgtggctc gtcttgtgat
      901 tggtgatcac ccgggaaccc gcgcggccgt ggccattgtc ttccgtgaat ctttggctga
      961 tgtttatttt agcttgagcg cagccaccgg caatccagaa tatgatgtga tgaggaagtt
     1021 ctggattcat tcaaatgatg aagcaaatac ggatttaaga cttaaagaag ccgtgttaaa
     1081 atcgtttgaa atcgttaacg gtcgttccgg gtttgatgtc caaatcaaaa gctatggtca
     1141 gcaacttctg tctttcgggg ggccgctgtt agcgccggat gcggggactt ccgtaagtat
     1201 gagggcgaat cgtaatgcgg aattaaagga gagtttggat ttagatggtc ttacggacta
     1261 caaattcgat cttgcgaact tcatcggcag cgcgaaaatt gtaaacaaca acggtttggg
     1321 ccaggttcgt ttagcagtga aaatgagaaa aagagtgggg tatcgtcaag agagcttcag
     1381 cttgacgttg atgcgaatta tcaagcctac gttgaatccc aatcaagaaa acctgaaatt
     1441 gaattaataa agcatccgtt ttttgtactg ttcatcgagg gcggccctat cccaaggtcc
     1501 gccctgtttt tttgttgtca tggcgaaaaa ccttgtggtc aactaggtgc agaaaaaagg
     1561 agctgatttc tatgcacggg acatcgtttg agtttgcgaa gaccacaact aattccaaca
     1621 cttccatttc cgcgccccat gtttcagatt acatggatta caggcagtat ttagcaagct
     1681 tttttgagta caaacgcaag caaacggctt cagatctgcg cccctacaac tatgcggctt
     1741 tctcggccgg tgcgaatatt aagtctccga actatcttaa gatgattatt gaaggtcgcc
     1801 gaaatctctc cggagaaatg gttttaaagt ttgccaaggc tttgggcttg aataaagagg
     1861 cgacagaaga tttcaatctt cttgtttctt atgggcaggc gacggatcct gctaatagaa
     1921 atatgtttct gaaagagctt tcagagcatc gtgtgcaggt aaagctcaag agcggtgaaa
     1981 tcgatcagaa gacctgggat aagattccga attgggcggc gtgggtgatt tacgccatgg
     2041 tcgatcaaga gggagtaagc tacgacacta aaaccttgag ggaactgctc cgcggaaaag
     2101 cttcggaaga tgagatcgag caagctctag aaagtctttt caaatccggc gaattggctc
     2161 aggatcccga gaccggaaag gtcaggaagg cccacactct gatagaagct gcggaagaca
     2221 ttccagtggc cctggtaagg aagctacagg ctcaattgat gtacttaggt ctagagtccc
     2281 tttatcagga tgcggccacg gaacgggagt ttgggacgct gacaatgagt ctgacaaagc
     2341 aagagtttga ggagatcaaa ttcaagcttc gccagatccg caagcagatc aataaagaca
     2401 actcaattgc acgagcgagt tcaaagggcg aaaaggttta tcagctcaac atccaacttt
     2461 ttccggtaac aaatgcgacg aataaatccg ctcatgttga aaaagtaagc aaggaagatg
     2521 cgtcgcacta aaatttaaga atcttaaaaa agtgcccgca aaaaacaata aattataggc
     2581 atgtctgtgg ataaacctgt ggattgcgtt gataactcat cgatttaagt tagttttgcg
     2641 acattttgtc acctgcccat gttagaatct tgtaaagcgg agatgggatt ttaggaaaat
     2701 gggggcattg cgacaccaaa atttttttaa ttttattctt gagatcagtt gaaactgcga
     2761 catgattact agttttttga aaatgtgata tagtcttgca attcaaaaaa atgtgggggc
     2821 gacacggctt cgacgtgggt gctgaagcat aaggagcata ccggggagta tgaggacctc
     2881 gtaaaaacat acactttgta attggcaacg attacgcact tgcagcttaa ttaagtttaa
     2941 gcgcacgatc ttctcaccca tggtggcggg gtgaggtctg atcgtcattt agtcaccacg
     3001 gctcgtttgg ttttcttcag cgcacgagct taaataattc tggggaaaat gttgcgcgcg
     3061 gattttgtcg atggaagccg cgcgaccaaa tcaaaacatc gactaagtat gtagcgcctt
     3121 atcgtggatc acttgcggac gggggttcaa ttccccccgc ctccaccaac aattttagtg
     3181 gttttaatgc tttagcatat gaaaaccacg tccgatttag acggggtaga cgatgaagtc
     3241 tacctcatct gaaaggaccc caatgggcta ctacatccga aaactcgctt ggaagaaatc
     3301 aaatcctcag tggaagctgc aatttgtttc atataaaaat tcagacacca aagattctgg
     3361 agctattaag ccaaaaa
//
LOCUS       DATBTE010000055        13698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_347709, whole genome shotgun sequence.
ACCESSION   DATBTE010000055 DATBTE010000000
VERSION     DATBTE010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 13698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13698
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347709"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VIG33_02245"
     CDS             complement(<1..217)
                     /locus_tag="VIG33_02245"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEY8269680.1"
                     /translation="MSNSTPQWHNIIKDLGPSLYRYFSASFATQTAADLVQETLIRLV
                     HKQRRGEFAAEKGSLKAYAFGIARFVRL"
     gene            377..1324
                     /gene="pip"
                     /locus_tag="VIG33_02250"
     CDS             377..1324
                     /gene="pip"
                     /locus_tag="VIG33_02250"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEY8269681.1"
                     /translation="MREYYPEIEPFETGRLKVCEIHELYYEQVGNPQGRPVVFLHGGP
                     GGGIHSDHRRFFDPQHYRIILFDQRGSGKSTPIAELRENDTWSLVADIEKIRAHLGIE
                     NWVVFGGSWGSTLALTYAITHPEKTAALILRGIFLCRPSEIHWFYQEGASHVFPDIWE
                     EYLKPIPKEEQNQLVKAYYKRLTSPHYPTRLEAAKAWSKWEAATSRLIVSREAMDEFD
                     DPDFAISFASIECHYFINNAFFSTNNWILENVHKIRHIPGFIIQGRYDMVCPARSAWD
                     LHKAWPEAKLSFTSDAGHAAAEPGTKSSLIEAAEACKVY"
     gene            1336..2127
                     /locus_tag="VIG33_02255"
     CDS             1336..2127
                     /locus_tag="VIG33_02255"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEY8269682.1"
                     /translation="MKIFKNFTLFFGIFMSACSPLKYSEYAEINSSEKENQPSNIISG
                     VEVDNNSTLSKSVVLLVNILTQEICSAALIGSQYALTATHCLDKEDPNNLFIFFAAKP
                     DFQTERRQVVAMRTPPSGNIALLKFEGETLPKGFEEASFLPVSEKIQKGARVIVLGYG
                     IKEAQSKAGAGILRYTTLTVSDPNYSSTEILLDQSKGSSVCHGDSGGPAYFLRKDSYG
                     QPKLYLWGIADHASLNDVNDQCNKGVIYINALVFLPWIENVLRTL"
     gene            complement(2124..2867)
                     /locus_tag="VIG33_02260"
     CDS             complement(2124..2867)
                     /locus_tag="VIG33_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8269683.1"
                     /translation="MEKIKIISLYGFLGLPADWDLIRSYLMVSPLAHQFEWWSVDYMN
                     TPGLDTECDFTAWAKNFNNSVQQKFAVGPRILLGYSLGGRLALHALKRNPELYDQAIF
                     ISTNPGLQSEKDKEERAKTDQRWSEQFLKTPWAELMSTWNAQAVFKDSLSEPLRLEAA
                     YDREKLARALVEWSLARQEDFQEFILQRGSQILWACGEKDIKFASQAMELQRSSSQLR
                     AEIFPRASHRVLFDQPHDLAQKIISFLIS"
     gene            complement(2855..4474)
                     /gene="menD"
                     /locus_tag="VIG33_02265"
     CDS             complement(2855..4474)
                     /gene="menD"
                     /locus_tag="VIG33_02265"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEY8269684.1"
                     /translation="MTNMQLAANVLQVLVSSGIREFCLCAGARNSPFVHILEENQRMG
                     LEENKDIKVYSFFEERSAGFFALGRMAKTQRPMAVITTSGTAVAELLPSAIEATYSSL
                     PLVIVSADRPKAYRGSGAPQSIEQVGIFSYYIEASLDIDAENTHFSVKGLTWKKPIHV
                     NVCFDEPLIDGSIMKLEMPSTSEWVKFPEQVEPTVGLETINFLQNHRPLVILGTLPSK
                     AQLPILAFLKKIRCPIYAEGISGLRGHSDLKALEIRSGENFIHRLLDQGTCDAIFRIG
                     GVPTVRLWRDLEDKRKSLPVLSVGYNHYSGLSRWVNHFTHLASLAAVEVPPQNPEHFK
                     DLGIVLKEDISLTEKKDQLLAKYPRSEPAMVWALSKKLKGQSVYLGNSLPIRHWDLVA
                     DFDSYPSRVVGNRGANGIDGQVSTFLGWAEASTENWCLIGDLTAMYDLSALWITPQLE
                     NKKMRIVVINNKGGQIFQRLFKKDIFLNRHDLEFNCWAKMFHWEYQSWETIPEGNQYK
                     TPLGEHQIIELIPDPQQTEAFHKEWDQVWKK"
     gene            complement(4471..5535)
                     /locus_tag="VIG33_02270"
     CDS             complement(4471..5535)
                     /locus_tag="VIG33_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEY8269685.1"
                     /translation="MKPLNFTNFLESGALLRPSPNRWLLLEGPFTEMAEVASGEVALF
                     HPDFFSEGVDNYWIPKSFYIVTDNDLKSLCADVDRPLLAPLNWQEADPQSFGVSFISI
                     LELIHRGALKKAVPIVFENSTGIMSSMRVRTLIKELIKAPPSLYVFGSWQKGSGILGA
                     TPEVLLKQEGRILQTMALAGTCPKEEAAERRLLLEDPKERYEHGLVVQDISQTLEKYG
                     RVRTEGPKILELPTLLHLKTEIECQLPKDQNFSFFEACRALHPTAALGVSPRDYGYEW
                     LKNLPEQRGRRGFGAPWGISWSAEEALCLVAIRNIQWNPGGCRIGSGCGIVAQSDLHQ
                     EWKELSQKRRSVKKVLGLES"
     gene            5703..6542
                     /gene="aroF"
                     /locus_tag="VIG33_02275"
     CDS             5703..6542
                     /gene="aroF"
                     /locus_tag="VIG33_02275"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY8269686.1"
                     /translation="MEIESMQTTPSTQIVKIKTAYGTLDIGGPQFSVFAGPCSIESHE
                     QFLETANTVKEHGACMLRGGIWKMRTSAKTFQGLGESSFDFIKSVCKATQRGLVSEIT
                     DARQIEELADVVDMFQVGSRNMHNYSLLKELGRTNKPILLKRGFASLIDEWIKAAEYI
                     TAGGNQNVILCERGIRTFETATRNTLDLNAVVYVKKNTHFPVIVDPSHAVGIRALVPD
                     LAIAAAAAGADGIIVEVHPRPAEALSDGPQALTFDDFKGMMAKIERVLAALDRPLQKS
                     AHS"
     gene            6552..7265
                     /gene="ubiE"
                     /locus_tag="VIG33_02280"
     CDS             6552..7265
                     /gene="ubiE"
                     /locus_tag="VIG33_02280"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEY8269687.1"
                     /translation="MQQKHSPNPEIIRNMFSKVAANYDKGNNVLSMGIHHLWRKKLVK
                     LSGAHPGQSILDCATGTGDLAIEFKKAVGDTGQVIGTDFCAEMLIPAPAKAAAKGLNI
                     RFEQADVTQLQYPDDTFDICSISFGIRNVGNPVKGLKEMARVTKPGGKVIVLEFGQVR
                     LPVFGPLYNFYSEKILPKLGGLVTGQGEAYEYLQRSSAAFPCREEFLPLMQETGDFSK
                     MNYIPLTGGIAYIYQGTVR"
     gene            7653..8552
                     /locus_tag="VIG33_02285"
     CDS             7653..8552
                     /locus_tag="VIG33_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269688.1"
                     /translation="MAKLFKMFQTKQYTFAMDRSAARRTDILNKNEFVNIHQAKRSAG
                     AYNPDARSQVEAEYAGLPLEEYNRLPDATKPKSMYLVPETSTGIDLAPTIYFQDNKTN
                     KYTGDTWIFALDKIERNTAFIVGDSYNRALIEREFADDFKQMIILPEAHFLKNNILDY
                     VLPLDLLLTAVPFYYEQVRTKNKFRFVNEDSAEYQKRFEDFKAEMDGFLADWQYNLIN
                     DLRPTFSAGFFEKFPELKPYEKSFLFRPYGNYVEGLYYGPLSVAGKVKALIFRSTPPT
                     AEEMAKFKELGIQVIDGRKRIKE"
     gene            8758..11793
                     /locus_tag="VIG33_02290"
     CDS             8758..11793
                     /locus_tag="VIG33_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8269689.1"
                     /translation="MNVAINTPSEDKVLDKTGKTRSRLIRLCGSLSTYLSLFSIVFPL
                     VSAAGWILGIPLLTQGLPSLPAMQPNTIFGLLLATIAVLFSQTKDQVTRNSFLACAIA
                     IIVSLLGAITLVEYFFDLSLEIDQIFIKGVATTMQPFPGRPSPQASLNFFLLGVAILS
                     FNLRFIPVFLGQVCAILIAINAVIVVTGYVFSTAHAYGFPIIKPSVGMAVHTAIVFIL
                     LAGALLCSRPNEGILTLVISDTRSGTWVRQILLASIFVPPFVGALARIGVILGWYDVS
                     MQISLFSVVMIGLILRTTWRSARQSEQEELRARAAYETILLTNEHLHQAIKQRQIFSA
                     LIENSSDFIGIADPNGKPQYLNPAGRRMIGLPVDYPVENTHIAEYYPPDQHSFVTDVI
                     VKSVAEQGQWSGETHLRHWQTQDSIAVSDTHFMIRDPESKLILGMGTIIRDIHEQKRI
                     ENEQRFLAEVGSVLASTLDYENTVENIVQLAVRDIADLCVVYVVEENGEVRRLKAISR
                     DPANKWICDLLTQKPFDPNQTSLIASVLENKREVLVEHLSPEMFAFLYQGEEHLRALQ
                     ATTPKSFMAVPFIIHGKVVGAIALLSSKSSRIYKSADLRLAKELAYRATLSIENARLY
                     REAQRAIKTREEVLAIVSHDLKNPLVAVELVAQLLPRLNQSDLQLLPEYANRIRRSTH
                     QMQKLIGDLLDFAKIQAGTFSIEKVVEKPIEVILPMVESVKTLAEAKRQRLVVEVPAG
                     LPEVSCDTYRISQVLSNLLGNAIKFTPEGGTVQISATAESDEILVSVSDTGPGILPEH
                     LSNIFDQFWQAQGTKQLGSGLGLSIAKGIVEAHGAKIWVESQIGKGSCFSFTIPLATS
                     ETKKRESPLLITSEEKPAEMGRHILDGINILLVDDARDIRLLLKHILEYAGAKVSEAQ
                     SATEAVLKLSQERPTMIITDIEMSEGNGYELIKKVRQMDEKDHLHLPVAALTGHTDHA
                     EIQKISEAGFDAHFSKSISADKLTSSIRKLINGPSDISV"
     gene            complement(11807..12823)
                     /gene="aroC"
                     /locus_tag="VIG33_02295"
     CDS             complement(11807..12823)
                     /gene="aroC"
                     /locus_tag="VIG33_02295"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY8269690.1"
                     /translation="MNANSFGARFVITTFGESHGAALGVVIDGCPANIVFDEALLKKE
                     LERRRPGGELVSQRKEEDTPEILSGVFAGKTLGTPLTIIVRNKDQKSQDYEQIKNAPR
                     AGHADDVWKLKFGHRDYRGGGRSSGRETVARVMAGAVARMVCMQLAPRMQVKAYSSQV
                     GHYMLSEAERGQVWFQDIEKFTARFPSVQHEKVKELLVQAQAQGESYGGVAEIRIKDG
                     PPGLGQPVFHKLKADLAQAYMSMGATAGIELGAGAEASHQKGTEFHGGFQSQNYGGIR
                     GGISTGEEILLKVHFKPTSSILDVAKKGRHDPCIVTRAIPVLEAMTWLVFADHLLWMK
                     MDQV"
     gene            complement(12820..>13698)
                     /locus_tag="VIG33_02300"
     CDS             complement(12820..>13698)
                     /locus_tag="VIG33_02300"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate synthase"
                     /protein_id="HEY8269691.1"
                     /translation="GYFWQQADPKNRSFLPRSINGRWTWFRLWMKGRQLLNFWREGEG
                     SAPDQPTIFEWLSVHFIKTHFAAILGKPVTNSWTPIEQKSFFEERNEPVFRIEMDKQE
                     WEKALGVLTHLGLRQAAVTSPLKGEAFYSCKEKTEKAEILRSVNTMFYNQERRCWVGH
                     NTDFVGFKSLTEDILSKQAIVLWGGGGTLNMLKKVFPQSYCFSASRGEVREEDQARWQ
                     KDMQPEVLIWAAPRNENLKWPFPEWKPKLVVDLNYTDDSAGREYALSLNAEYRSGLKM
                     FRVQAQHQREFWENQE"
BASE COUNT         3746 a         3298 c         2942 g         3712 t
ORIGIN      
        1 ctaaccttac aaaacgtgca attccaaagg catatgcctt caagctccct ttttctgccg
       61 caaactctcc gcggcgttgt ttatgcacaa ggcgaatcaa agtttcctgc acaagatccg
      121 ccgccgtctg ggtagcaaaa cttgccgaaa aatatcgata gaggctggga cctaagtcct
      181 tgataatatt gtgccattgc ggagttgaat tgctcatagt tgtaattgga attaaaagcc
      241 taaaaagttg caatggcgag aaaataaacc gcctccatct ttctctagac tccgagaaaa
      301 aaatctgaaa aattaagaac ccggtaactc tgaagcgtcg catccgaaaa aggtacggcg
      361 tacccgaaag gtttttatgc gtgagtatta tcctgagatt gaaccctttg aaactggccg
      421 tttgaaggtc tgtgaaattc atgagcttta ttatgagcag gtgggaaatc ctcagggtcg
      481 gcctgttgtg tttcttcatg gggggcctgg cggagggatt cattcagatc atcgccggtt
      541 ttttgatccg caacattatc ggatcatttt atttgatcag cgtggctctg gaaaaagtac
      601 gccgatcgcg gagcttcgtg agaatgatac ctggagcctc gtcgcagaca ttgaaaaaat
      661 tcgtgctcat ctggggattg agaattgggt cgtcttcggc gggagttggg gatctacctt
      721 ggccctcact tatgcgatca ctcatcctga aaaaacggcg gctttgattt tgcgtgggat
      781 ttttctgtgt cgtccttctg agatccactg gttttaccag gagggggctt cccatgtttt
      841 ccccgatatt tgggaagaat acttaaaacc tatccctaaa gaagagcaaa atcagttggt
      901 gaaagcttat tataagcgtc taaccagccc tcattatccc acgcgcttag aagccgcaaa
      961 ggcttggagt aaatgggagg ccgcgacctc tcgcctgatc gtcagtcgcg aagccatgga
     1021 tgagtttgat gatcccgact tcgccatttc attcgcaagc attgagtgtc attactttat
     1081 aaataacgct ttcttctcta ccaataattg gattctcgaa aacgttcata aaattcgtca
     1141 cattccagga tttatcattc aaggccgcta cgatatggtg tgccctgccc gcagcgcctg
     1201 ggatttgcac aaagcatggc ccgaagcaaa actcagtttt acttcagatg cgggacatgc
     1261 ggcggctgag cctggcacta aaagttcttt gattgaagct gcggaggctt gcaaagttta
     1321 ttaaggggga cgacagtgaa aatatttaag aatttcactc ttttttttgg catattcatg
     1381 agtgcctgct ctcccttaaa atattctgag tacgctgaga tcaactcctc cgaaaaggaa
     1441 aaccagccct caaatattat ttcaggcgtt gaagttgata ataacagtac tctgtctaaa
     1501 agcgttgtgc ttttagttaa tattcttaca caggaaatct gttcagcggc tttgattggg
     1561 agtcaatacg ccctgacagc gactcattgc ttagacaaag aagatccgaa taatcttttt
     1621 atattttttg cagcgaaacc agattttcaa acggagcgac gccaggtggt tgcgatgagg
     1681 acacctccgt cgggaaacat cgctcttctt aagttcgaag gtgaaacttt gcccaaggga
     1741 tttgaagagg ccagcttttt acccgtcagc gaaaaaattc aaaagggagc gcgggtcatc
     1801 gtcttaggtt acggtattaa agaggcgcaa tcaaaagcag gagccggaat tttgcgctat
     1861 accactttaa cagtctctga tcctaactat agttctacag aaattctttt ggatcaaagc
     1921 aaaggctcct cagtttgcca tggggactct ggcggccctg cttattttct taggaaagat
     1981 tcttatggcc agcccaaact ttacctctgg ggtattgcgg accatgcttc tctaaatgac
     2041 gtgaatgacc aatgcaataa aggagtgatt tatatcaatg ccttggtctt tctgccttgg
     2101 atcgaaaatg tcttgaggac cctctaagag atcaaaaagg aaattatctt ttgcgcgaga
     2161 tcatggggtt ggtcaaaaag aacgcggtgc gaggctctcg gaaaaatctc agctcggagt
     2221 tgggaagaac ttcgctgaag ttccatcgcc tggctggcaa atttaatatc tttttcgcca
     2281 caagcccaca gaatttgtga gcctcgttgt aaaataaatt cctggaagtc ttcttgccga
     2341 gccaaagacc attccaccaa ggcccgtgct aatttttcgc gatcataggc tgcttccaaa
     2401 cgcaaaggct cactcaggct gtccttaaaa acagcttgag cattccacgt gctcataagt
     2461 tctgcccaag gagtttttaa aaactgctcc gaccaccgct ggtcagtttt cgcacgttct
     2521 tctttgtctt tttcactctg aagccccgga ttggtagaaa taaaaatcgc ctgatcgtag
     2581 agttctggat tcctcttcag agcgtgcaaa gccagacgcc cacccaatga atagcctaac
     2641 aaaatacgag gtcctactgc aaatttctgc tgaacagaat tgttaaaatt tttagcccaa
     2701 gccgtaaagt cacactccgt atccagccct ggagtgttca tataatccac ggaccaccat
     2761 tcaaattgat gggccaaagg ggaaaccata agatagcttc ggattaaatc ccaatctgcc
     2821 ggaagtccta gaaagccata aaggctgatg atttttattt tttccaaacc tgatcccact
     2881 ctttgtgaaa ggcttcggtt tgttgagggt ctggaatcaa ttcaatgatt tgatgttccc
     2941 ccaatggagt tttatactga ttcccttcgg gaatcgtctc ccaagactga tactcccaat
     3001 gaaacatttt agcccaacaa ttaaactcga gatcatggcg gttcaggaaa atgtcctttt
     3061 taaacaagcg ctggaaaatt tggccgcctt tattgttaat caccacgatc cgcatttttt
     3121 tattctcaag ctgaggagtg atccacaagg ctgacaaatc atacatggcc gtcaagtcac
     3181 caatcagaca ccagttctca gtcgaggctt ccgcccaacc taaaaacgtg gacacttgcc
     3241 catcaatccc attggctcct cgatttccaa caactcgtga gggataggaa tcaaaatccg
     3301 caactagatc ccaatgccga atgggcaagc tgtttcctaa gtaaacggat tgtcccttaa
     3361 gttttttcga taaagcccaa accatggcag gctctgaacg cgggtacttc gccaagagct
     3421 gatctttctt ttcagtaagg ctgatatctt ctttgagaac aatacccaaa tctttaaaat
     3481 gttctggatt ttgcggtgga acttcaaccg ccgccaatga ggctaagtgt gtaaagtgat
     3541 tcacccaacg gctcagaccg gaataatgat tatagcctac tgaaagaacc ggaagacttt
     3601 tgcgtttatc ttcaaggtct cgccacaatc taacggtagg cacaccccca atacgaaaaa
     3661 tggcatcaca agtcccttga tccaaaagac gatgaataaa attttcaccc gagcgaatct
     3721 ctagggcctt aaggtctgaa tggccgcgaa gtcctgaaat cccttccgca taaataggac
     3781 agcgaatttt tttaaggaaa gccaatatcg gcaactgcgc cttcgacggc agagttccga
     3841 gaataaccaa gggtcgatga ttctgtaaga agttgattgt ctcaagcccc accgtgggct
     3901 ccacttgctc tgggaacttt acccactcac tcgtgctcgg catttccaat ttcatgattg
     3961 agccatcaat caatggctca tcaaaacaca cattcacgtg aataggcttt ttccaagtca
     4021 aaccctttac cgagaagtga gtgttttcag catcaatatc caaagaagct tcgatataat
     4081 aagagaaaat acccacttgc tcaatactct gcggcgctcc cgatccacga taggccttgg
     4141 gccgatccgc tgagacgatg actaagggta atgaagagta cgtggcctca atggccgacg
     4201 gtaaaagctc ggccacggcg gtgcctgaag tcgtgataac agccatgggc ctttgggtct
     4261 ttgccatacg gcccagagca aaaaatcccg cagagcgttc ttcgaaaaaa gaataaacct
     4321 taatatcttt attctcttca agacccatcc tctgattctc ttccaagata tgcacgaagg
     4381 ggctattcct ggctcccgcg cacaaacaga actcacgaat acccgacgag accaaaactt
     4441 gcagcacatt ggctgccaat tgcatatttg tcatgattcc aatcccaaca ccttttttac
     4501 agatcttctt ttttgcgaaa gctctttcca ctcttgatgt aaatcacttt gagcgacgat
     4561 gccacatcct gatccgattc tacatccacc aggattccat tgaatattac gaatagcaac
     4621 caggcacaga gcctcctccg ctgaccagga aattccccag ggcgctccaa acccacggcg
     4681 tcccctttgt tcaggcaagt ttttcagcca ctcataacca taatcccgag gagaaacacc
     4741 gagagcggca gtaggatgta gggcacggca ggcctcaaag aatgaaaaat tttggtcctt
     4801 aggaagttgg cattcaattt ctgtttttaa atgaagcaaa gtgggcaact caaggatctt
     4861 cggcccctcc gtcctaactc ttccgtactt ctcaagagtt tgcgatatat cttgcaccac
     4921 caacccatgt tcataacgtt ctttggggtc ttccaacaaa agcctgcgct ccgccgcctc
     4981 ttccttagga caggttcccg ccaaagccat ggtttgcaaa atgcgcccct cttgcttcaa
     5041 taaaacttcg ggagtcgctc caagaattcc gctccccttc tgccacgagc caaacacata
     5101 gagacttggg ggagccttga tgagctcctt gattagagtt ctgactctca tcgagctcat
     5161 gatccctgta gaattttcga aaactatagg aactgctttt ttaagcgctc cccgatgaat
     5221 gagttctaaa attgatataa aagaaacacc aaaactttga ggatccgctt cttgccaatt
     5281 cagaggagct aataaggggc gatcgacatc ggcacataag gatttaaggt cattatcagt
     5341 aactatataa aaagactttg gaatccaata attatcgacc ccttctgaga aaaaatcagg
     5401 atggaataaa gccacttctc ccgaagccac ttcggccatt tcggtaaagg ggccttctaa
     5461 caaaagccac cggttggggc tcgggcggag gagagctccg gattctaaaa agtttgtaaa
     5521 atttaatggc ttcatgctag tctgcgcttc cacaaaaaac cccggttttt tgccctaaag
     5581 ggttaacttt ttctgactag aagggtcaat aaatttcgcc tttggatcag tagctgggta
     5641 ggaaatggtt agtttattgc ttagttattt gcttcaccaa gctagccagg gaagttcgta
     5701 atatggaaat cgaaagtatg caaacaactc cctcaaccca aatcgtcaag atcaagacgg
     5761 cttatgggac tctcgatatt ggaggcccac agttttctgt tttcgccggc ccttgctcca
     5821 tcgaaagtca tgaacaattt ttggagacag ccaacaccgt aaaagagcac ggcgcttgta
     5881 tgcttcgtgg tggcatttgg aaaatgagaa cctcagctaa gacctttcaa gggctgggcg
     5941 agtcttcttt tgattttatc aaatccgtct gtaaagcaac ccaacgcggc cttgtttctg
     6001 aaatcaccga cgcccgtcaa attgaagaac tcgcagatgt cgtggacatg tttcaagtgg
     6061 gctctcgcaa tatgcataac tactcacttc taaaagaact tggtcgcacc aataaaccca
     6121 ttcttttgaa acgtggtttt gcttctttga ttgatgaatg gattaaagcc gccgagtaca
     6181 tcacggcagg gggaaatcaa aatgtcattc tctgtgagcg tgggattcgc acttttgaaa
     6241 ccgccacccg caacaccttg gaccttaatg cggttgtcta cgttaagaaa aacactcatt
     6301 tcccggtgat cgttgaccct tctcatgcag tgggaattcg tgccttagtg ccggatctgg
     6361 cgatcgcggc ggcggccgca ggcgctgacg gtattatcgt ggaagttcat cctcgtccgg
     6421 cagaggcttt gtctgatggt ccgcaagcgc tgacctttga tgacttcaaa ggaatgatgg
     6481 cgaaaataga aagagtgctg gctgctttag accgtccttt gcaaaaatct gctcactcct
     6541 aaggagactt cgtgcaacaa aaacactcgc ccaatcctga aatcattcgc aacatgttca
     6601 gcaaagtcgc tgcgaactat gataaaggca acaatgtgct ctccatggga attcaccatt
     6661 tgtggcgcaa aaaattagtg aaactcagcg gcgctcatcc gggacaatct attcttgact
     6721 gcgccacggg cactggggat ttggcgattg aattcaaaaa agctgtgggt gacaccggac
     6781 aagtcattgg tacggatttc tgtgcggaaa tgctgattcc agcccccgct aaagccgctg
     6841 caaaaggtct gaacattcgc ttcgaacaag cggatgtgac tcaactgcaa tatcctgatg
     6901 acacttttga tatctgcagt atctcctttg gaattcgcaa tgtcggaaac cctgtaaagg
     6961 gtcttaaaga gatggcccgt gtgacaaaac ctggtggcaa agtgatcgtt cttgaattcg
     7021 ggcaagtgcg cctaccagtg tttggacctc tttataattt ctattctgaa aagatcctcc
     7081 ctaaactcgg cggtctggtc acaggccagg gcgaagcata tgagtatctt caaagatctt
     7141 ctgcggcttt cccttgccgt gaagaattcc tcccattaat gcaggaaact ggggatttca
     7201 gtaaaatgaa ttatatccca ctcactggcg gcattgccta tatctatcag ggaactgtaa
     7261 gataaatttg gcctgaaatt tggccactgc ctgacaattc ttgcggttaa catttaagct
     7321 ttatcaaatt acattggctt gacttaaatc cgatttaaac atagccgggg acttttctca
     7381 tttcaagaca aggtccacgg tttgcaatag tcaaatgtat gaaactcatt cccattttat
     7441 tcatttttat ttttcaattc ccagcgctgg gagcaataaa gtgcgaacaa ctcctattaa
     7501 gtgacgtaac atctcagcag gcattacaaa gtcagactga aagcgttgtt aaacacctta
     7561 gacaacatac tagtatgggc atctctccct ttcaaaccga aataaaagcc tattttaacg
     7621 ctctccaaaa atccgcaaca catgatcctg tcatggcaaa gcttttcaag atgtttcaaa
     7681 ccaaacaata cacctttgcc atggatcgta gcgccgctcg aaggacagat attttaaata
     7741 aaaatgaatt cgtcaatatt caccaagcta agcgttccgc tggcgcctat aacccagacg
     7801 cacgatctca agtagaagct gagtatgcgg gccttccgct tgaagaatat aatcggttgc
     7861 cagatgcgac caaacctaaa tcaatgtatc ttgttccaga aacctcgaca ggcattgacc
     7921 ttgcaccgac tatttatttt caagacaata agacaaataa atacaccggt gacacctgga
     7981 ttttcgcttt agataaaatt gaacggaata cggcctttat agtcggggac tcctataaca
     8041 gagccttgat tgaaagggaa tttgcagacg atttcaaaca aatgatcatt cttccggagg
     8101 cacattttct aaaaaataat attctggatt atgttctgcc actagatctg cttttgaccg
     8161 cagttccttt ttactatgag caagttcgaa ctaaaaataa atttagattt gtgaacgaag
     8221 attcggctga gtatcaaaag cgttttgaag attttaaagc ggaaatggac ggatttttag
     8281 ccgattggca gtacaattta ataaacgatt tgcgacccac attcagcgct ggttttttcg
     8341 aaaaattccc tgaactaaaa ccttatgaaa aatctttttt atttcgaccc tatggaaact
     8401 atgtcgaagg actttattat ggacccttat cagttgcagg aaaagtgaaa gccttgattt
     8461 ttcgctccac tccccccacc gcagaggaaa tggccaagtt caaagaatta ggtattcagg
     8521 tgatcgatgg tcgaaaaaga attaaagaat aatatctgct cgattgtcag gggccatttg
     8581 cgataattct acaagagacc tcagtctctt ttccaactgt cctgattata cgacaattca
     8641 tatttgcttc gcgttaaaaa catgacttat gtattgtata gaggaagacg ttggctctgc
     8701 tctagcacag atcaggtgct tgaagaatga tccctcttct tacttctaag gtggaccgtg
     8761 aacgtagcaa tcaacactcc ttctgaggat aaggtactag acaagaccgg taaaacaaga
     8821 tcccgcctga ttcgactttg tggatcactt tcaacatacc tgagtctttt ttctattgta
     8881 tttcctctcg tgagcgctgc gggttggatt ctcggcattc ccttgttgac acaggggctg
     8941 ccgtcattgc cagcaatgca gccaaatacg atctttggcc tattgctagc gaccattgct
     9001 gtacttttct ctcaaacaaa agaccaggtt accagaaatt cattccttgc ttgcgcaatt
     9061 gccataatcg tttccctttt aggcgccatc accttagttg aatatttctt cgacttgtcc
     9121 ttagaaatcg accaaatttt catcaaaggc gtcgccacca caatgcagcc ttttccaggc
     9181 cgcccctcac cccaagcatc actcaacttt ttcttgctcg gtgtagcaat tctttccttt
     9241 aatttacgat tcattcctgt ttttttaggc caggtttgtg caatattaat agcgatcaac
     9301 gccgttattg tagtgacagg ttacgtcttt agcacagctc atgcctatgg ctttcccatt
     9361 attaagcctt ccgtcgggat ggctgttcat accgctatag tctttattct attggcagga
     9421 gccctgctct gctctaggcc aaatgaggga atacttactc tcgtcatcag cgacacgcgg
     9481 agtggcacct gggtaagaca gattctcttg gccagtattt tcgttccccc gtttgttggt
     9541 gctttggccc ggattggagt gattttgggg tggtacgacg tgagtatgca aatttcctta
     9601 ttttcagtag tgatgatagg actcatactt cgaacaacgt ggcgatccgc tagacaatca
     9661 gagcaagaag aacttcgagc cagagctgct tatgaaacga tcctgcttac gaatgaacac
     9721 ttgcatcagg ccataaagca acgtcagata ttttccgccc ttatcgaaaa ctcttctgac
     9781 tttattggca tcgcagatcc caatggaaaa ccccaatatc tcaacccggc cggccgtcgc
     9841 atgataggcc tgccggttga ttaccctgtg gagaatacac acatagctga atactaccct
     9901 cccgatcagc attcgtttgt aactgatgtg attgttaaat cagtggccga gcagggtcag
     9961 tggagcggtg agacccatct ccggcattgg caaacccaag attccatcgc ggtttctgat
    10021 acgcatttta tgatccgcga tcccgaatct aaactgattc tcgggatggg gaccatcatt
    10081 cgtgacatcc atgagcaaaa acgtattgaa aacgagcaaa ggttccttgc ggaagtaggt
    10141 tcagtattgg cttcgactct tgactatgaa aacaccgtgg aaaacattgt tcagttggca
    10201 gttcgcgata ttgcggatct atgtgtcgtg tatgtggtcg aagagaacgg cgaggtccgt
    10261 cgattaaaag cgataagccg agatccggca aataaatgga tctgcgattt gctcacccaa
    10321 aaacctttcg atccaaacca gacaagtctg atcgcctcgg ttctggagaa taaacgtgag
    10381 gtcttggttg agcacctttc acccgaaatg ttcgccttct tatatcaggg cgaagagcat
    10441 ctgcgagcgc ttcaagccac tacccctaaa tccttcatgg cggtgccgtt cataatacat
    10501 ggaaaagtgg taggtgcaat tgctctcctg tcttcaaagt catcgcgtat ctataagtcg
    10561 gctgatcttc gcttggctaa agaactcgct taccgtgcca ctctttctat agagaatgcc
    10621 cggctctatc gagaagcaca acgcgctatt aaaacacgcg aggaagtgct agccattgta
    10681 tcgcacgatc ttaagaatcc cttggtagcg gttgaactcg tagcgcagct cctgccgcgg
    10741 cttaatcaaa gtgatcttca actgttgcct gaatatgcaa atcgtattcg gcgctctact
    10801 catcagatgc aaaaattaat cggcgattta ttggattttg ccaagatcca agccggtaca
    10861 ttctcaattg aaaaagtcgt ggaaaaaccc atcgaagtca tcttaccgat ggtcgaaagc
    10921 gttaaaacct tggctgaagc taagcggcaa cgtctcgtgg tggaagtccc ggcaggactt
    10981 ccagaggtca gttgtgacac ctatcgcata agccaagtcc tctcgaatct tttgggaaac
    11041 gcgatcaaat tcactccaga aggagggact gtacagattt cggcaacagc agaaagtgat
    11101 gaaatactag tttcagtttc tgacaccggt ccgggaatcc tccccgagca tttatcaaat
    11161 atatttgacc agttctggca ggcccaaggc acgaagcaac tgggaagtgg cttgggcctt
    11221 tctattgcaa aaggaatcgt tgaagctcac ggcgctaaaa tatgggtcga aagccaaatt
    11281 ggaaagggca gttgtttttc attcaccatt cctcttgcga cgtccgagac aaagaaaagg
    11341 gaatctcctc tactgataac aagtgaagaa aagcccgctg agatgggaag acatattctt
    11401 gatgggatca atattctact ggttgatgac gctcgagaca tacgtcttct cctgaagcat
    11461 attctcgaat acgctggggc gaaagtctcc gaagcacaat cagcaaccga ggctgtcttg
    11521 aagctttctc aagagagacc tactatgata atcacggata ttgaaatgtc agagggcaat
    11581 ggatacgagc ttatcaagaa agttcgacaa atggacgaaa aagaccatct ccacttaccg
    11641 gtggcggcct taactggtca tacagaccat gcggaaattc aaaaaatatc cgaagctggt
    11701 ttcgatgctc atttttcaaa atcaatttca gcggacaaat taacttcatc cattcgaaaa
    11761 cttattaatg ggcctagcga tatttcagta taattataat cattgcctaa acctgatcca
    11821 ttttcatcca aagcaaatgg tcagcaaata ccagccatgt catcgcctcc agaaccggaa
    11881 tcgctctggt cacaatgcat ggatcatgac gacccttttt cgctacatca agaatcgagg
    11941 aggtgggctt gaaatgcact ttcagtaaaa tttcttcacc ggtggaaata cctccacgga
    12001 ttccaccata attctgtgat tgaaacccgc cgtgaaattc ggtgcctttt tgatgggaag
    12061 cctcagctcc agcgcccaac tctatgcctg cggttgctcc catactcata taggcttgcg
    12121 ccaaatccgc ctttaactta tggaagacgg gttggccaag acccggaggt ccatctttaa
    12181 tgcgaatctc tgcgactccg ccatagcttt ctccttgggc ttgagcttga actagaagct
    12241 ccttcacttt ttcatgttga actgaaggaa agcgagcggt gaacttttca atgtcttgaa
    12301 accatacctg accacgctct gcttcagaaa gcatatagtg acccacttgg ctggaatagg
    12361 ctttcacttg cattcggggg gcaagttgca tgcacaccat ccgcgctacg gcccccgcca
    12421 tgacgcgtgc gacggtttcg cgtcctgaag atcttcctcc tccacgataa tcgcggtgcc
    12481 cgaacttcag tttccataca tcatccgcat ggccagcacg aggagcgttt tttatttgtt
    12541 cgtagtcctg ggatttttga tctttatttc tgacaataat ggtcagaggg gtgcctaagg
    12601 tttttcctgc gaagacccca ctcaaaattt ctggtgtatc ttcctctttt cgttgcgaca
    12661 cgagttcacc gccgggccgt cgacgctcca attccttttt taaaagagct tcatcgaaaa
    12721 cgatgtttgc aggacaaccg tcaataacca ctcccaacgc ggcgccatga gactcgccaa
    12781 aagtggttat cacgaatctg gctccaaagg aatttgcatt cattcttgat tctcccaaaa
    12841 ttctctttga tgctgagctt ggactcggaa catcttgagc cctgaacgat actccgcatt
    12901 taggctcagt gcgtactcac gtcccgccga atcatccgta taatttaaat ccaccactag
    12961 cttaggcttc cactcaggaa aaggccactt gagattctca ttgcgagggg ccgcccagat
    13021 taagacttca ggctgcatat ctttttgcca acgcgcttgg tcctcttcac gcacctctcc
    13081 tcggcttgca gaaaaacaat aagattgagg aaatactttt ttaagcatgt tcaaggtccc
    13141 accgccaccc cagagaacaa tggcctgttt ggagagtatg tcttcagtca gacttttaaa
    13201 tcccacaaag tccgtgttat ggcccaccca acatctgcgc tcttgattat aaaacattgt
    13261 gtttactgat cgcaaaattt ccgctttttc ggttttctct ttacaactgt aaaaagcctc
    13321 tcctttaagt ggagaagtca ctgcggcctg acgtaatccc aaatgggtca aaactcctaa
    13381 ggctttttcc cattcttgct tgtccatttc aattcgaaaa acaggttcat tgcgctcttc
    13441 aaagaagctt ttctgctcta tcggagtcca actgttggtg acaggcttgc caaggatagc
    13501 cgcaaaatgt gtcttaataa aatgaacact caaccattca aaaatcgtcg gctgatcagg
    13561 ggcactgcct tcgccctctc gccaaaaatt caaaagttga cgacccttca tccataatcg
    13621 aaaccaggtc caccgtccat tgattgaacg aggtaaaaaa cttcgatttt ttggatcggc
    13681 ttgctgccag aaataacc
//
LOCUS       DATBTE010000056        16205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_483748, whole genome shotgun sequence.
ACCESSION   DATBTE010000056 DATBTE010000000
VERSION     DATBTE010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 16205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16205
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483748"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..1812)
                     /locus_tag="VIG33_02305"
     CDS             complement(268..1812)
                     /locus_tag="VIG33_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269692.1"
                     /translation="MIIYSFKVTFLSLIFLMASHQVQAQSLDLSLEEGNHTWAKSESW
                     TWMGHLNEYTTSGQGTLSLSENGLALIHYYTRNQTQFGSLTFKSSALTYTKKGIGEFY
                     DKRTIYSEPPISKNKFQLKIQESHLNIEFKHNEQIDQIRKTSISESDIHLLKNKLLPL
                     YPGLKMSENFDPVKYTFSAFVGSETFLSLELYQIWPSISPKDAKMQFLGIPEAYTFSQ
                     PMLVAVGSSRKKGISTPVVGLLFLDRQWCKDYFGKNIMGNPIESLQQNQALKYSHSWS
                     AFHAHSEQSQNWYFVHLWRQYQRNPDVPDKIDDYSGIVWVKNGIQQTPIEDKSFNWIG
                     SKFIKNKSHVMLNYAVDRETYFPSQYHLYNKANADSLSLQASPRLQSLDQPIYLYEGY
                     ASGGGTWDGESVKLQGRVESSRTLFRLQDYIEMLGQIKQSRAISSVEDPEQEKLGQHL
                     EDIIAREKNCKNNWLCFSDFLEKRKAQIGYMLNDLKQKMEIFQSGFKSTQPVRDAADH
                     SIVIYN"
     gene            complement(1899..2858)
                     /locus_tag="VIG33_02310"
     CDS             complement(1899..2858)
                     /locus_tag="VIG33_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269693.1"
                     /translation="MNILKLAIMVVFSLSGGVFAGVNSQFSLELKVEASLESKVHQLV
                     SERLAPNHGFSKDEIEALVHDLAVLASINGGKPSALYKTIFSQNTFSGSGFFQWLITG
                     IKQLKASHGEPCTPLSKVDYFGCSNISARSITLPPEYFQNNRIRRILTLIHERRHLDG
                     YDHIPNTPSYDKEVKGTRGAQLAFIVSLVNSCTNCTIMAKQEALSNKEEIVGAMKNLS
                     EDDSQLLQIELASLQNIIPVEVSTFLGELKRQQSFLSVSHMECKNKVPLYEYEQHRTV
                     PNVDAQDCFIIYYKKGKSPHLGEQFPEPITRFGVLFLFVPTAK"
     gene            complement(2990..4021)
                     /locus_tag="VIG33_02315"
     CDS             complement(2990..4021)
                     /locus_tag="VIG33_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269694.1"
                     /translation="MKIAHLLPLCLFTLNFLALTSETVWASFDDYQHFRKGTYDLGIE
                     TQYFKTEANYASSGSSYHSLPYGQSYDIFNAYLMVRHELSKRSAWYGNLDIANATSYG
                     VGYTRSNSAISDLKLGYAYRPYSDLFDVVTDFNVLIPFNEIDPNTDTVLNNEGVGEAT
                     GLLRIQREFSTVSTFGYIGGTFRQSRSSLLPWGLGVEASYLKWGWGGKIFGYQSITED
                     PDTVNKTQRLIVTDRVDAGSAKFYSVNPSLVDSEVFLKFKIQNAWTLTTGGGMTITGS
                     NTGAGFHVGIGLLYSWDSEPSYYLTPFSTQEDDLGSEKKVPKFKEEIDDGVNQKLFQK
                     KVTPPRSGT"
     gene            4071..5291
                     /locus_tag="VIG33_02320"
     CDS             4071..5291
                     /locus_tag="VIG33_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8269695.1"
                     /translation="MSTPPNKSLLAIIFFTVFIYLVGFGILIPIVPILSRDFGATALQ
                     TGLLMSVYSFMQFVFAPFWGTLSDRMGRRPILLFCLVGEACSYLIFAWARSLEWLFVA
                     RILAGFFGASISTASAYISDITPPNERSKGMALIGAAFGLGFVVGPALGGGLALWGQH
                     ISDAPHFQTSFCALWVGALCMGGFFFGLKFLKESLTEKNKNTHSKKRFQLLKHYLAQK
                     TMGAMLSVYFLSSLAMSGMEATLILFMAEKFGWQLQQVSFGFAYIGVITVLSQGVFVR
                     RLIPKLGERKILRIGLASFAIGLTGIAFAPTIPWMGVTMTLLALGTSFTNPATLGSIS
                     LLAGTSEQGVTMGVAQSLASLGRILGPALGGIVYEKIAINSPFLVSGFLALSGLTTVI
                     IISKNLPDSGKKVS"
     gene            5293..5637
                     /locus_tag="VIG33_02325"
     CDS             5293..5637
                     /locus_tag="VIG33_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8269696.1"
                     /translation="MAQSIGFFQFDNLVKSRIGFIFINLGVDTSALYHHMEKSHLENT
                     LLRLPEGELANVSTATIVAALANQAKDSAVIVLSPDGVTSLEVATALEKAGFLNVFDV
                     KGGWKQILADQD"
     gene            5957..7297
                     /locus_tag="VIG33_02330"
     CDS             5957..7297
                     /locus_tag="VIG33_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY8269697.1"
                     /translation="MISILSFTTLAMNGCDAIYWNESESASQVHNDQPEIKVLTLLNP
                     LTYSTDKTGHKFGIDHDLLENFAEQYNLRVQFIPVANEVEMKIALAAGIGDIAAARLA
                     TPQDNHDFLVGPTYEETFLALFCQTRAQVANVADLKNKKIAILSKDNLHGFTQRLRKL
                     APQSRIQVTVSASPKILFERMARKQVDCVMAENLEGEFYARYYHKVEKVTPLTKNYSL
                     SWLIRPHLTDLNRLLQAWFQRASRNDEIMRVHDRYKLYLSELDRQDVLHFMKQTRLTL
                     PDFERQFRRAGHENNLPWQLIAAVSYQESKWNSAAVSYTGVRGIMQLTQETAAHLGVE
                     DRNDPLQSIWGGAKYLRYLMNKTPPYLNFKDRLCLTLAAYNMGYGHLRDAQKLLARQG
                     KNPYSWRHLREALPLLENSSYEPELEFGLARGSETIEFVERVSGFYNLMVATQL"
     gene            7507..8349
                     /locus_tag="VIG33_02335"
     CDS             7507..8349
                     /locus_tag="VIG33_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269698.1"
                     /translation="MKSFKMRYFFIAPMVVLAIVACAKRDSDFAKRAEDARNAANKTT
                     PPTSPTPSPTTDLKAPSPAPADPANPTTDTSEDQDGTKDLYKESCKNPIVMEVQEDET
                     ELAIDDLVSKEKSSYILQSSEFYVDHAVKDAKENSLPKNQLHVTGSQYELPKDFDKTA
                     TDENKILVICHTVKADKTSTQKMSDNFILPYEISAADGSVTVLRQDKIDIDSKATIKK
                     VSTLYSQKFSLKQLLADPQVKQSLIVKHSDGSIVIKLQLTNTEDKTDAFISATYTLKE
                     PAKK"
     gene            complement(8390..10273)
                     /gene="glmS"
                     /locus_tag="VIG33_02340"
     CDS             complement(8390..10273)
                     /gene="glmS"
                     /locus_tag="VIG33_02340"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY8269699.1"
                     /translation="MCGIVGYFGPQNPKDVIVSGLKKLEYRGYDSAGIAILHEGKTKR
                     VRASGKLKALEDKLINEKFDGQIGIGHTRWATHGAPTERNAHPHQVKGINLVHNGIIE
                     NYMEIREDLIAKGATITSDTDSELVAHLIANEVEVTQDLHKAVERILSKIRGAFSIVV
                     MWEKNPNQLVAFKDGPPLVVGLGSGEAFVASDVQALLQYTHKFIYLDDREIALINGVE
                     VKILSAAGVPIEKKTVELNWDPQTVEKQGFSHFMLKEIYEQPRAVAAAIEPHLNVESF
                     SIRLKNVGFGALVNKLDELDSQQDWKKTQEVLKGIDRIFIIACGTSSYAGMVGKYLME
                     NIAQVPVEVDIASEFRYRNPVIPPKTLVITISQSGETADTLAAIRLAKEKGATTLSIC
                     NVPSSTIDREAHGHLYMNSGPEIGVASTKAFTSTLAVLNCFAVAMGRTRNVIDALKEK
                     EFAQALLAAPSQMEVVLAYDKYFDEAAHKLRQFRGFLYLGRGVSFPIAMEGALKLKEL
                     AYMHAEGYAAGEMKHGPLALIDERMAIVMLAPHDELYEKSMSNLEEARARGGQVIAIG
                     TGDNEKLKSISQYYLSLPKTHWIINPLLEAIPLQLMAFHLASSLGYDVDQPRNLAKSV
                     TVE"
     gene            complement(10278..11705)
                     /gene="glmU"
                     /locus_tag="VIG33_02345"
     CDS             complement(10278..11705)
                     /gene="glmU"
                     /locus_tag="VIG33_02345"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEY8269700.1"
                     /translation="MAKNDTKEAAQLNPLPNKLAMIILAAGKGTRMKSPLPKVLHPVA
                     GRPMIEKVIQASKKAGATDLRVVVGHGQNLVRSVVEPMGVTCFAQEHQLGTADAVKAA
                     KVEDIEGDVIIMNGDHPLIEAEDIKKFLQIFRDEKCDLAVVTAEVKKPGVFGRVVRHM
                     GSLKAIVEAKDASSDTLKIREINSGIYVAKASVLTEYLPQINNHNSKNEFYVTDLISL
                     CIEDGCKVMAIKASPKVAVGVNDQMELAHATKLLFRRKTKRLMEEGVLMIDPTTTYVE
                     ESVEVGAGSVLYPNTVLRGSTKIGSFSVIEPNVFISDSQIGNSVQIRAGSYIEQTVIF
                     DRASVGPYARLRPQTSIGEEAHVGNFVEMKKVKFGKKSKAGHLTYLGDAEVGEEVNIG
                     CGTITCNYAADKKKYPTIIGNRVFVGSDTQFVAPIEVGDDAVIGSGSTITKNVPAKAL
                     AVARGKQVIKENYMAKAPAVPVSEE"
     gene            11754..12470
                     /locus_tag="VIG33_02350"
     CDS             11754..12470
                     /locus_tag="VIG33_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY8269701.1"
                     /translation="MQAKIIHQTSWARSSRGTPLELYKKTHNRDQKATDPILFAPILF
                     VGGVHGDEPEGVRLAQEFLIWLRKTETESSEKILHSWLLVPCLNVDGYLRNERTNDRG
                     VDLNRNFPSRDWSSEAKAPRYYPGARAGSEAETQAMVQLIETEKPKLIVHFHSWEPCV
                     VYTGAPGKEASTILAGNTGYEVREDIGYPTPGSLGQYGWLDHKIPVICIEEQERIPLN
                     QVWSHFEQGLTQLMMTDFSL"
     gene            12479..13165
                     /locus_tag="VIG33_02355"
     CDS             12479..13165
                     /locus_tag="VIG33_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY8269702.1"
                     /translation="MTAIKCIAFDLDDTLVDTSQLLVPMAARRACEEMINHGVKSDID
                     TCLAWRASQTADLTHQEIFQRFIAKYGSTTPNVALQEAMKAFYNPEVPAKLPLMEGAE
                     TNLKKLAERYNLFLVTSGEPEAQMKKVKALGIEYSFRKIYLVNNFKHQTKKEAFVDIL
                     SSESLQPEGLLSIGNRLSQEIRYAKLCGARTCYFCHGEHVGEQPTQREDYPDFTIYKH
                     SELIKACGLG"
     gene            complement(13252..13653)
                     /locus_tag="VIG33_02360"
     CDS             complement(13252..13653)
                     /locus_tag="VIG33_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269703.1"
                     /translation="MKAGARKPGNFGTSLNSNWTVTTKEIEPHTPSFSFPLGSESKAT
                     YYFISKDDRCVLDFVEVEQSFNGNKIGASLTVEKCLEYKKEKLTINNPDYSGKYGYKG
                     NLCKDIEKYFRTAESVEPKAKTKSTESRGMR"
     gene            complement(13650..13868)
                     /locus_tag="VIG33_02365"
     CDS             complement(13650..13868)
                     /locus_tag="VIG33_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269704.1"
                     /translation="MKKVVSMWFLLFFSNNVYAETCLEVSKEVVKQFGYNELRCKNSE
                     SKCNLDLGNPNSPEFEISHKGLRVNYLL"
     gene            complement(14116..14547)
                     /locus_tag="VIG33_02370"
     CDS             complement(14116..14547)
                     /locus_tag="VIG33_02370"
                     /inference="COORDINATES: protein motif:HMM:NF025838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4431 domain-containing protein"
                     /protein_id="HEY8269705.1"
                     /translation="MKSLFLSVFLFLSSSLSFARNQVLTYGPSTVELEGILDLQVFPG
                     PPNYESIKSGDEIERHFYLKLTSPIDVLPKGKHPGVENVEEERNVRILQLAIDGENDE
                     LWARFRKVGKGSHVKILGTLFHRFTAHHHSRVLLSVKSMEP"
     gene            complement(14544..15212)
                     /locus_tag="VIG33_02375"
     CDS             complement(14544..15212)
                     /locus_tag="VIG33_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3892 domain-containing protein"
                     /protein_id="HEY8269706.1"
                     /translation="MPKYTKAEPGQGHSVIYTDADGKQWKFVGGSRPWRNQNPGNLVP
                     GNVSKRNGTIGAAGGFAIFPDYETGHCALLDSLKNVHGNKDIALLMKVYAPPKENDTK
                     KYIAFMRKKIGIKGNKKIKEFSDVEFEKLWRAIEQMEGWGKEGTILEYSVKGEITAVK
                     RDKKGTIQSYQIEGYGWVSKSEGVALTLDGKVEAVVATSPKGNQFLRARPNSESSDNL
                     ENKG"
     gene            15365..15970
                     /locus_tag="VIG33_02380"
     CDS             15365..15970
                     /locus_tag="VIG33_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8269707.1"
                     /translation="MALHSNRKKTARKGSQLSFFNKKIPRVFGGSLLKGNAKVQRPLS
                     TKESIHLVLKSGQALGAYSMLQQRNAGKIDDIIRFHAKKCGVKIYHFVNVGNHLHLVI
                     KLSDLKLFAKFIRTITGLIARHVLKKQRGPKQEDSPALGSKKTPSFWVARPFTRLIAW
                     GKDYNFVAGYMKKNRNQAKRYFIPWGFDVVDPDLIQFLNTG"
BASE COUNT         4425 a         3759 c         3420 g         4601 t
ORIGIN      
        1 ttctagcggc gatcttaaag gtttcttggc tgctcagcgg actggaaaga gctgatactg
       61 ctattaaaag cggaaggagg actaataaaa catgtttcat agaaagcctc gtttaaaatg
      121 aactttaagc tagggattac cagagtcgat aatgcgagat ttgttccaag ctagctatgc
      181 agttattttg aaacggagag ctgctgctgt ctaagttttt aaattggatc cacaaatgtc
      241 actttggcga aaaatataga attctgacta attatagatc acgatcgaat ggtctgctgc
      301 atctcgcacc ggttgagtcg acttaaaacc gctttgaaag atttccattt tttgttttaa
      361 atcattcaac atataaccta tctgcgcctt tcttttttct aaaaaatcag aaaaacaaag
      421 ccaattattt ttacaattct tttcgcgggc aatgatatct tctaaatgct gacctaactt
      481 ttcttgctcg ggatcttcaa cggagctgat cgcacgtgat tgcttgattt ggccaagcat
      541 ttctatgtaa tcttgaagac gaaagagtgt tcggctcgac tccacccgac cctgcaattt
      601 cacggattca ccatcccaag ttcccccgcc ggaagcatag ccttcataga gatagatagg
      661 ctgatccaag gattgcagtc tcgggctcgc ttgaagcgat aagctgtcgg cgtttgcttt
      721 attatataag tgatactggg aagggaaata ggtctcccta tccacagcgt aattgagcat
      781 gacgtgtgat ttgtttttaa taaattttga accgatccag ttaaaagatt tatcttcgat
      841 gggggtttgt tggattccat ttttaaccca tacgatccct gaataatcat cgattttatc
      901 cggaacatct gggtttcttt gatattgccg ccacaaatga acgaagtacc agttctggct
      961 ttgctctgaa tgcgcatgaa aggcggacca actgtgcgag tattttagag cttggttttg
     1021 ttgaagactt tctatcgggt tccccatgat gtttttaccg aaataatcct tacaccattg
     1081 gcgatctaaa aagagcagtc ccaccaccgg agtggaaatc cccttttttc gagaggagcc
     1141 aacggcgact aacataggct gtgaaaaagt ataggcctct ggaattccta ggaactgcat
     1201 tttagcgtct ttgggagaaa tggagggcca tatttgataa agctccaggc ttaaaaaagt
     1261 ctcgctcccg acaaaggctg aaaaagtata tttcactgga tcgaagtttt cgctcatttt
     1321 caatcccgga taaagcggca gtaacttatt ttttaaaaga tgaatgtcac tctcgctgat
     1381 ggaagttttt cggatttgat cgatttgctc gttgtgttta aactcgatat ttaaatgact
     1441 ctcttggatt ttgagctgaa atttattttt acttatggga ggctcggaat aaatggtcct
     1501 tttgtcatag aactcgccaa tccccttttt tgtgtaagta agagccgagc ttttaaaagt
     1561 taaactgcca aattgagtct gattgcgcgt gtagtagtga atcaaggcca aaccattttc
     1621 actgaggctc agcgtgccct ggcctgaagt cgtgtactca tttaagtggc ccatccatgt
     1681 ccaagactct gacttagccc aagtatggtt tccctcttct aaggataggt ccaaggattg
     1741 agcctgaacc tgatgagatg ccatgagaaa aataagactt aagaaagtga ctttaaaaga
     1801 ataaattatc atattccaga ccttgtaaga gttcgtcccc tactttcggc aggaatagga
     1861 ctcgctaaag tctagtttta taaacaaggt tcttgccgct atttagcggt tggaacaaac
     1921 aaaaacagca ctccgaagcg agtgatgggt tcaggaaact gttcgccaag atgaggcgat
     1981 tttccttttt tgtaatagat aataaagcaa tcctgcgcat ccacattcgg taccgtccga
     2041 tgctgctcat attcatataa cggaacttta ttcttacatt ccatatgact gaccgagaga
     2101 aaactttgct gtctctttaa ttcacctaag aatgtggaga cctcaaccgg tattatattt
     2161 tgaagagaag ccaactctat ctgaagaagc tggctatcgt cttcagatag attcttcatt
     2221 gctcccacaa tttcctcttt attactgaga gcctcttgct tagccatgat tgtacaattt
     2281 gtacagctgt tcactagtga aacaatgaaa gccaattggg caccccgagt gcctttcacc
     2341 tctttgtcgt agctcggagt gttaggaata tgatcgtaac catcgaggtg cctgcgctca
     2401 tgaatcaagg ttagaattct tcgaattcga ttgttctgaa aatactccgg aggaagggta
     2461 atactccggg cacttatatt ggaacacccg aagtaatcca ccttgctcaa aggagtacag
     2521 ggttcaccgt gggaggcctt caattgcttg atccctgtga tcagccattg aaaaaaacca
     2581 gatccagaga aagtattctg agaaaatatc gttttataca atgcagaagg cttgccgccg
     2641 ttgattgagg ctaagaccgc aagatcatga accagagcct caatctcgtc tttagaaaat
     2701 ccatgattcg gagcgagtcg ctcactgacg agttgatgga ctttactttc gagggaagcc
     2761 tccactttca gctcaagcga aaactgagag tttacgccgg caaagacacc cccgctcaaa
     2821 gaaaaaacca ccattatagc taacttcaat atattcatac ggccccaata tgctagattc
     2881 aggcctggct caaagatggt ttatttttaa ttgataggtg agaagtgtct aattctcttt
     2941 tttttggcgc tgattgtccc tgcataaata taaatttttc aaatattttt cacgttccgg
     3001 atcgtggagg ggtgactttc ttttgaaaaa gcttctgatt aacaccatca tcaatttcct
     3061 ctttaaattt tggaactttt ttctctgaac ctaaatcatc ttcttgtgtc gagaagggcg
     3121 tcaaataata gctgggttcg ctgtcccaag aatagagaag accaatgccc acgtgaaaac
     3181 ccgcccctgt attagatccc gtgattgtca ttccaccgcc ggtggtgaga gtccaagcgt
     3241 tttggatctt aaactttaaa aaaacttcag aatccaccaa agagggattc actgaataaa
     3301 atttcgcact gccggcatcc acgcgatccg tgacgatgag gcgttgggtt ttgttaacgg
     3361 tgtctggatc ctcagtaatg ctctgataac cgaaaatttt tccaccccac ccccacttaa
     3421 gataagaggc ttccacccct aatccccaag gcaaaagtga cgagcgactt tgacggaagg
     3481 tcccgccaat ataaccaaaa gtagataccg tggagaactc tctttggatc cgtaaaagcc
     3541 cggtcgcttc tccgacgcct tcattattca aaacagtatc cgtattcgga tcgatttcat
     3601 taaaagggat taagacatta aaatccgtaa ccacgtcaaa caaatcgcta tagggccgat
     3661 aagcataacc taattttaaa tcacttatgg cggagttcga gcgggtataa ccaactccat
     3721 aactagtggc attggcaatg tcgagattcc cataccaagc acttctcttg ctcagttcat
     3781 ggcgaaccat taaataagca ttgaagatat cataagattg gccgtaaggt agactatgat
     3841 aagaacttcc agagcttgca tagtttgcct cggttttaaa atactgggtt tcaatcccaa
     3901 ggtcgtaagt tcctttgcga aaatgttggt aatcgtcgaa cgaggcccac acagtttcac
     3961 tggtcagtgc tagaaaattt agtgtaaata agcatagagg caaaagatgt gcaattttca
     4021 taactctccc atagtaacag attaacagga acagaaccga ggtaaagaac ttgtctaccc
     4081 cgccaaataa atccctactt gctatcatat tttttactgt tttcatctac cttgtgggct
     4141 ttggaatatt aattccaatt gttcccatct taagtcgaga tttcggagca actgctctgc
     4201 aaaccggcct tttgatgtcc gtctattcct tcatgcaatt tgtcttcgcc cccttctggg
     4261 gaaccctgag tgatcgcatg ggacgtcggc ccatcttgct tttctgtctc gtgggcgaag
     4321 cttgctccta ccttattttt gcatgggcca gatcccttga atggctcttt gtggcccgga
     4381 ttctcgctgg ctttttcggt gctagtattt ccaccgcttc tgcttatatt tcagacataa
     4441 ccccgcccaa tgaaagatcc aaagggatgg ccttgattgg agccgccttt ggtttaggtt
     4501 ttgtggtcgg tcctgcttta gggggaggct tggctctctg gggccagcat atttccgatg
     4561 ctcctcactt tcaaacaagc ttctgcgccc tttgggtagg ggctttatgt atgggtggtt
     4621 tcttctttgg tttgaaattc ctcaaagagt ccctcaccga gaaaaataaa aacactcata
     4681 gcaaaaaacg ttttcaactc ctcaagcatt acctagctca aaaaacaatg ggcgctatgc
     4741 tcagtgttta ttttttgtcc tctttagcca tgtcaggaat ggaggcgact ttgattttat
     4801 ttatggctga aaaatttggt tggcagctcc agcaagtcag ttttggcttt gcttacattg
     4861 gagtgatcac ggtgctgtcg caaggagttt tcgtacggcg gctgattcct aaattggggg
     4921 aaagaaaaat tttgcggata ggcctggcca gctttgccat cggactgact ggcatcgcgt
     4981 ttgcaccgac gattccctgg atgggagtaa cgatgacctt gttagcatta ggaaccagtt
     5041 tcaccaaccc cgccactctc ggaagtatca gcctcctcgc gggtacgagc gaacaaggag
     5101 tcaccatggg agtcgcacaa agtttagctt ctctgggcag aatcttaggg cccgccctcg
     5161 ggggcatcgt ttatgaaaaa atcgcaatca attcaccctt tttagtttca ggatttttag
     5221 ctttgtctgg actaaccact gtgattataa tttctaaaaa ccttcccgac tctgggaaaa
     5281 aagtgagtta acatggcaca gtccattggt ttttttcaat ttgataatct ggtgaagtcg
     5341 cgtatcggtt ttatctttat caatcttggc gtcgacacca gtgctcttta tcatcacatg
     5401 gagaagagcc atttagagaa taccctctta cgattgccgg aaggcgaact cgcaaacgtc
     5461 agcactgcca ccattgttgc cgctcttgcc aatcaggcaa aagacagcgc tgtcattgtg
     5521 ttgagccctg acggagtgac atctttggaa gttgccactg cgctagagaa agcaggattc
     5581 ttgaatgtct ttgatgttaa aggcggttgg aaacaaatcc ttgcagatca agattagttt
     5641 catgctctgg cgcagatcta gaaattttct ccgtatctct tcctcaagat agtcctcagt
     5701 gttgcaaact ctaaaaatgg tctgatttgg aatgaccatc aaatcagagc aaacgcgtaa
     5761 ttctccataa aaaagcccct gttttccgtg ggtcttaagc ccttgttccc tctgtatttt
     5821 tttcattcat tgccttatga ctctattttt cggcgttttt tacggtaact gcataagaaa
     5881 tcgcccgcta atgcttgccc aatggccccc taaggtctag accgacccct gcatgatgag
     5941 attcaagcgt ttgataatga tcagtattct aagctttacg actttggcaa tgaatggctg
     6001 tgatgctatt tattggaatg agtccgagag tgcctcgcag gtccataatg accagccgga
     6061 aatcaaggtt ctaactctat tgaaccccct gacctattcg accgataaaa caggacataa
     6121 attcggtatt gaccatgatc tgctcgaaaa ctttgcggaa caatacaatc ttcgagtgca
     6181 gtttattccc gtcgcgaatg aagttgaaat gaaaatagct ttggctgcag gcatcggtga
     6241 tattgccgca gctcgcttag ccactccgca agacaaccat gattttttag ttggtcctac
     6301 ttacgaagaa actttcttag ctcttttctg tcagaccaga gcccaagtgg ccaatgtggc
     6361 tgatctcaaa aataaaaaaa tcgcgatcct ttccaaggac aatcttcatg gctttacgca
     6421 aagattgcgt aagctggctc cccaatccag gattcaggtc acggtttcag catcgccaaa
     6481 aattttgttt gaacgaatgg ctcgcaaaca agttgattgc gtgatggcgg aaaatcttga
     6541 gggagaattt tatgcgcgtt attatcacaa ggtagaaaaa gtgacgccct taaccaagaa
     6601 ctattccctg agttggttga tccgccctca cctgacagat ttgaatcgct tgctccaggc
     6661 ctggtttcag cgagccagcc gcaatgatga aatcatgcgt gtgcatgatc gctataaact
     6721 ttatctttca gagctggatc gccaggatgt cttgcatttt atgaaacaaa ctcgtctgac
     6781 attgcccgac tttgaacggc agtttcgcag agcgggacac gaaaacaatt taccgtggca
     6841 gctgatcgcg gcagtctctt atcaagaatc caagtggaac agtgccgccg tcagttacac
     6901 aggggtccgt ggcatcatgc aactcactca agagacggcc gcgcatttag gagtagagga
     6961 tcgcaatgat cctctgcaaa gcatctgggg cggagctaag tacctgcgct atttaatgaa
     7021 taagactccg ccttatttaa attttaaaga tcgtctttgt ttaactttag ccgcctataa
     7081 tatgggctat ggccatctcc gagacgctca aaaacttcta gcgagacaag gtaaaaatcc
     7141 ttattcttgg cgccatttgc gagaagcttt gcctttgctg gaaaattctt cttatgaacc
     7201 ggaattagaa tttggcctcg cccgcggcag cgaaaccatt gaattcgtgg aacgagtcag
     7261 tggtttttat aaccttatgg tggccactca gctgtaatcc ttagctgtaa cttttacggt
     7321 gatttacggc ataatccttg aaacattcct tctgtgtcga caacgagggt attcagatac
     7381 aatacactcg ttataagtcc ctctaaaccg ccactgactg gtaaatattt cgacagaggc
     7441 agcgtaaaag ggctcaagga cacgtacttc acacacgtac ctaacgtaag acttaaggag
     7501 aggaaaatga aatcattcaa aatgcgttac ttttttatag cccccatggt ggttttggca
     7561 attgttgctt gtgcaaaacg cgacagcgat tttgctaaac gggctgagga tgcgagaaac
     7621 gctgcgaata aaacaacgcc tccgacttca cctactcctt caccgaccac ggatctaaaa
     7681 gcaccgagtc cagcccctgc agatccggca aatccgacca cggacacctc agaggatcaa
     7741 gatggcacaa aagatctcta taaagagagc tgtaagaatc cgatcgtcat ggaagtccaa
     7801 gaagatgaaa ccgaacttgc gattgacgac ttggtaagta aagaaaaaag ctcatacatt
     7861 cttcagtctt cagagttcta tgttgatcat gctgtgaagg acgctaaaga aaacagcttg
     7921 ccgaaaaacc aattgcatgt cacagggtca cagtatgaac ttccaaaaga ttttgataag
     7981 acagcaactg atgaaaataa aattcttgtt atttgtcaca ctgtaaaggc tgacaaaact
     8041 tcgactcaaa agatgagtga taacttcatt cttccttatg agatcagcgc tgcagacgga
     8101 tccgtcactg tgcttcgcca agataaaatt gatatcgaca gcaaagccac tattaaaaaa
     8161 gtttcaacac tttactctca aaaattcagt ttgaaacaac tgttggcgga tcctcaagtt
     8221 aaacagagcc tcatcgtaaa acattctgac ggcagcatcg tcataaagct tcaattgaca
     8281 aataccgaag ataaaacaga tgcctttata tctgcaactt atacacttaa agagccggct
     8341 aaaaaataat taagcctgct ctttcttttc attgattatt ctctctctct tattccacag
     8401 tgaccgactt cgcaagattg cgaggttggt ccacatcata gcccagactg cttgctaaat
     8461 gaaaggccat caactgcaaa ggaatcgcct ctaaaagagg gtttataatc cagtgggttt
     8521 ttggcaaaga cagataatac tgactaatag acttcagctt ctcattgtca cctgttccaa
     8581 tcgcgatcac ctgaccccca cgggcgcggg cttcttcgag gttgctcatg gacttttcat
     8641 agagttcgtc gtggggagcc aacatcacaa tcgccatacg ctcgtcaata agagccaaag
     8701 gaccgtgctt catttcacct gcggcataac cttcggcatg catataggcc aattccttca
     8761 gtttgagggc tccttccatc gcaatcggaa aactcacgcc acggcccaaa taaagaaatc
     8821 cacggaactg acggagcttg tgggcagctt catcgaaata tttgtcataa gccagaacca
     8881 cttccatttg actgggggcc gccagaagcg cctgcgcaaa ttccttttct tttaaggcat
     8941 caatcacatt gcgagtgcgc cccattgcta cggcaaaaca atttaaaaca gccaaggtgc
     9001 tggtaaaggc tttcgtgcta gcgacgccga tttccggacc tgaattcata taaaggtgac
     9061 catgggcctc acgatcaatg gtcgagcttg gcacattgca aatactgagg gttgtggccc
     9121 ccttttcttt cgctaaacga atggcagcca aggtgtctgc ggtctcacca ctttgggaaa
     9181 ttgtgatgac caaggttttc ggtgggatga cagggttgcg ataacggaat tcactcgcta
     9241 tatcgacttc cactggaact tgggcaatat tttccattaa atatttaccg accattcccg
     9301 cataactgct ggttccacag gcgatgataa aaatcctgtc gatccctttg agcacctctt
     9361 gggtcttttt ccaatcttgt tggctgtcga gttcatccaa cttgttgacc aaggcaccaa
     9421 agcccacatt ttttaaacga atcgaaaaac tctctacgtt caagtgcggc tctatcgccg
     9481 ccgccacggc tcgtggctgc tcgtaaattt ctttcagcat gaaatgcgag aagccttgtt
     9541 tttcgacagt ttgcggatcc cagttgagtt ctacggtttt tttctcaatg ggaacacccg
     9601 cagcggaaag aattttaacc tcaactccat tgatcaatgc gatctcgcga tcatcaagat
     9661 aaataaattt atgggtatac tgaagaagcg cttgcacatc actggcgaca aaagcctcac
     9721 ccgaacccag tcccaccaca agtggagggc catctttgaa agccacgagt tgatttggat
     9781 ttttctccca catcacgacg atggaaaagg ccccacggat tttactgaga atccgttcga
     9841 cggccttgtg taaatcctga gtgacttcca cttcattggc gatcaggtgg gcgaccaact
     9901 ctgaatccgt atcggaggtg atcgtagcgc cctttgcaat aaggtcttcg cgaatctcca
     9961 tataattttc aatgataccg ttatgaacta aattaatgcc cttcacttgg tgaggatgag
    10021 cattgcgttc tgtgggagcc ccatgagtcg cccagcgagt gtggccaata ccgatttggc
    10081 catcgaattt ttcattgatc agtttgtctt caagggcttt taattttcca gaggcgcgaa
    10141 cgcgttttgt tttcccctca tgtaaaatgg cgatcccggc agagtcataa ccgcggtact
    10201 ccaatttttt aaggccggag acaatcacat ccttggggtt ttgcggaccg aaataaccaa
    10261 ctattccaca cataatttta ttcctcgctc acaggcactg ccggagcctt cgccatgtaa
    10321 ttttctttaa taacttgttt gccccgagcg acagccaaag ccttcgctgg aacgtttttg
    10381 gtaatcgtcg aacctgagcc aatcaccgca tcgtcaccga cttcaatcgg agccacgaac
    10441 tgcgtgtcac ttccaacaaa cacgcggttg ccgatgatgg ttggatattt ctttttgtca
    10501 gcggcgtagt tgcaagtgat ggtgccacag ccaatgttca cttcttcacc cacttccgca
    10561 tcgcccaaat aggtcaaatg gcctgctttc gatttcttgc cgaattttac tttcttcatt
    10621 tccacgaaat tacccacatg ggcttcttcg ccaattgaag tctgcgggcg aagtcgagca
    10681 taaggtccca ctgaagctcg gtcaaagatc accgtttgct caatatagct cccagcacgg
    10741 atctgcacgc tgttgccaat ttgggaatca gagataaaca cattaggctc aatcacagaa
    10801 aaagatccta tcttcgtgct gccacgaagc accgtgtttg gataaagcac cgaacctgcg
    10861 cccacctcca cggactcttc tacataagtt gtggtgggat caatcatcaa aacgccctct
    10921 tccatcaagc gtttggtttt acgtctgaaa agaagcttgg tcgcgtgcgc aagttccatt
    10981 tgatcattca ctccaacggc caccttagga gaagctttaa tcgccatgac tttacagcca
    11041 tcctcgatgc ataaagaaat caaatccgtc acgtaaaatt catttttaga attgtgattg
    11101 ttaatttgcg gcaagtactc agtcaacaca ctggctttgg cgacatagat tccgctattg
    11161 atctcgcgaa tttttaaagt atcggaggaa gcatcttttg cttccacgat cgccttcaag
    11221 gaccccatat gacgaaccac tcgaccaaag acgcccggct ttttcacttc agcagtgacc
    11281 actgctaaat cacatttctc atcacggaaa atttgcagga attttttaat atcctcggcc
    11341 tcgatcaaag gatgatcacc gttcatgatg atgacatctc cttcgatgtc ctccaccttt
    11401 gccgctttca ccgcatccgc agtgcccagt tgatgttctt gggcgaagca agtcactccc
    11461 atgggctcca ccacggaacg cactaaattt tggccgtgac ctacgaccac tcgcaaatcc
    11521 gtggctccag cctttttaga agcctggatg accttttcaa tcatgggacg acccgctaca
    11581 ggatgcagca ctttgggaag aggtgacttc atacgggtcc ctttacctgc cgccaaaata
    11641 atcattgcca gtttgttggg gagaggattc aactgtgccg cttccttcgt atcatttttt
    11701 gccatggaat ttctgtcctt ttcaagtctc aaaaaatcag gcttaattat tatatgcaag
    11761 caaaaattat tcaccaaact tcttgggccc gaagctcccg cggcactcct ctggaattgt
    11821 ataaaaaaac acacaaccgt gatcaaaaag ctacagatcc gattctgttt gctcctatcc
    11881 tgtttgtcgg tggtgttcac ggtgacgaac ccgaaggcgt ccgcttggcg caggaattcc
    11941 tgatttggtt gcggaaaacc gaaactgaat cttcagaaaa aatccttcac tcttggctgc
    12001 tggtcccctg cctgaatgtc gatggttatc ttcgcaatga acgcaccaat gacaggggcg
    12061 ttgatttgaa tcgcaatttt ccttcacggg attggagttc agaagccaaa gcacctcgct
    12121 attatccagg cgcccgtgct ggcagcgaag cggaaaccca ggcgatggtc cagttgattg
    12181 aaactgaaaa acccaaactg attgtgcatt ttcactcctg ggagccttgc gttgtttata
    12241 caggagcccc tggaaaagag gcttcgacaa ttttggcggg caacactggc tacgaggttc
    12301 gtgaagacat tggttatccg acgcctggca gccttggaca atatggttgg cttgatcaca
    12361 aaattcccgt gatctgcatc gaagaacaag agagaattcc cctcaatcag gtgtggtccc
    12421 acttcgagca aggcctcacg caactgatga tgacggattt ttcgctctaa ggactccgat
    12481 gaccgcgatt aaatgcatcg cttttgattt agacgacacc ctggtagaca ccagccagtt
    12541 gctggtgccc atggccgccc gtcgtgcctg tgaggaaatg atcaaccacg gagttaaaag
    12601 tgatatagac acttgtttag cttggcgtgc aagtcagaca gcggatctca cgcatcaaga
    12661 aatcttccaa cgattcatcg ctaaatatgg ctctaccact ccaaacgtcg ctttgcagga
    12721 agcgatgaaa gctttttata atcccgaggt gccagctaaa cttccattga tggaaggggc
    12781 ggagaccaat ctcaaaaagc tcgcggaaag atataatttg tttttagtga cctctggaga
    12841 gcccgaagct cagatgaaaa aagtcaaagc actgggtatt gaatactctt ttcggaaaat
    12901 ctacctggta aataatttta aacaccagac taagaaagag gcctttgtgg atattctcag
    12961 cagtgaaagt cttcagcccg agggactttt gagtattggc aatcgcttat cgcaagaaat
    13021 ccgttatgcg aagctctgcg gggcacggac ttgttacttt tgccatggcg agcacgtggg
    13081 ggagcaaccc acgcagcggg aggattaccc ggactttacg atttacaagc atagcgaact
    13141 gatcaaggct tgtgggttgg gatgaggggc tttggggatg aaaaaacact gatttaaagt
    13201 cggtgttttt tgatgcagct ctaggaatgg ttcttaggga ttttaatcag tttacctcat
    13261 accccttgat tcagtagatt ttgttttcgc cttaggttca acagattctg ctgttcgaaa
    13321 atatttttca atatctttac aaagattccc tttgtatcca tatttacctg aataatcagg
    13381 gttatttatg gttagctttt cttttttata ttccaggcat ttttcaacag ttaaagaagc
    13441 acctatttta ttgccattga aagactgttc aacttcgaca aaatctagaa cgcatctatc
    13501 atctttggat ataaagtagt aagtggcctt tgattcactt ccgagcggaa atgaaaagct
    13561 tggagtatga ggctctattt cttttgtagt tacagtccag ttggaattca atgaggtgcc
    13621 aaaattacca ggttttcttg caccagcttt cacagtaaat aattgacgcg aagtcctttg
    13681 tgagaaattt caaactctgg agagttcgga ttacctaaat ctaagttgca tttgctttca
    13741 ctgtttttgc aacgaagctc attgtaacca aattgcttca ctacttcttt agaaacttcc
    13801 agacatgttt cagcataaac gttatttgag aaaaaaagaa gaaaccacat cgatacaact
    13861 tttttcataa agttctccat cattaaagct catcggcatt tttatttatt gattgatatg
    13921 gcctttgtct aggaataata atctaaaatc aaataacaat aagccagata aggaacacaa
    13981 acatactggc ttattgaacc aggctcttaa agacgtggta atgaaaaaac caactcactt
    14041 gtgtataaaa tatttccagg ccgcaacgat ttttattttt ccttcagcta ctttcaaagc
    14101 accatgcttc cgcagctaag gttccatact ttttacactg agcaaaaccc tcgagtgatg
    14161 atgcgcagta aagcgatgaa acagagtccc aagaatcttt acgtgggacc ctttgcctac
    14221 ctttcggaag cgagcccaga gctcgtcatt ctccccatca atggccaact gcaggattct
    14281 gacattcctc tcttcttcga cattttccac gcccggatgc tttccttttg gcagcacatc
    14341 gataggcgag gttaacttta aataaaaatg ccgttcgatt tcatcgccgc tcttaatact
    14401 ttcgtaattg ggaggaccag gaaagacttg caagtctagg attccctcca attccacagt
    14461 tgagggtcca taggtaagaa cttgattgcg agcaaatgat aggctggaag acaggaatag
    14521 gaatacagat agaaaaagag acttcatccc ttgttctcca aattatctga agattccgaa
    14581 ttaggacggg ctcgcaaaaa ctgatttcct ttcggcgagg tggcaacaac agcttcaacc
    14641 ttgccatcta aagtaagcgc aacaccttcg cttttggaga cccaaccata tccctcgatt
    14701 tgataggatt gaattgtacc tttcttatct cgttttacgg cggtaatttc acctttgacc
    14761 gaatactcca aaatagtacc ttctttaccc catccttcca tttgctcaat ggctctccag
    14821 agtttttcaa attctacatc tgaaaattcc ttgatctttt tattgccttt tattcctatc
    14881 ttcttacgca tgaatgctat gtacttcttt gtgtcgtttt cttttggcgg tgcgtagacc
    14941 ttcatcaata aagcaatgtc tttattacca tgcacatttt taagagaatc aagtagcgca
    15001 caatgacctg tttcataatc tggaaaaata gcaaaaccgc cggctgcgcc aatagtgcca
    15061 tttctcttgc ttacgtttcc tggcacaagg tttccagggt tctgatttcg ccatggtctg
    15121 cttccgccca cgaacttcca ttgttttccg tcagcgtctg tataaatcac tgaatgacct
    15181 tgtccaggct ctgccttcgt atatttgggc atatttttca ctctccctac cattcgtcag
    15241 cgtggattct actcttttac ataaagaatc cgatgtcaat ttcgatataa aaatatctgc
    15301 aataaaattg acatcgctac cgtaatgcca ttaagatcgc aactcaatca gggaatatga
    15361 caagatggcc ttgcacagta acagaaagaa aactgctaga aaaggatctc agctctcttt
    15421 ctttaacaag aaaattccac gagttttcgg cggatctctc cttaaaggga atgcgaaagt
    15481 acaacgccct ctttcgacga aggaatctat tcacctggtg cttaagtcag gacaagcctt
    15541 gggcgcctat tcaatgttac agcagcgcaa tgccggtaaa atcgacgata tcatccgatt
    15601 ccatgcaaaa aaatgtggtg taaaaatcta tcactttgta aacgtcggga atcacttaca
    15661 cctggtgata aagttgagtg atcttaaact ctttgcaaag tttatccgta ccatcaccgg
    15721 gcttattgca agacatgtat taaagaaaca acgagggccg aagcaagaag acagcccagc
    15781 attagggtcc aaaaagacac cttctttttg ggttgctcgt ccttttacac gtcttattgc
    15841 ctggggaaaa gactacaact ttgtagctgg gtatatgaaa aaaaatagaa accaggcaaa
    15901 acgctatttt attccctggg gctttgatgt cgttgatcct gatcttattc aattcttgaa
    15961 caccggctag acctagttcc agttagacct gcgcaagaaa tgaaaaataa accctgccat
    16021 tgcgtctttc tttccgtaaa atcaaacgca tgcggatgat gaaaaccagt tttttattga
    16081 tcggtccatg ggaatcgagg cttgtcgagc tcggagctca aatcctcccc gatcttactc
    16141 acgcgttgaa gtggagcaaa gaggcccttt gcgacgtggt ggcgctgccc gcagtcgttc
    16201 tgctt
//
LOCUS       DATBTE010000057         1784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_347818, whole genome shotgun sequence.
ACCESSION   DATBTE010000057 DATBTE010000000
VERSION     DATBTE010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347818"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..>1784
                     /locus_tag="VIG33_02385"
     CDS             23..>1784
                     /locus_tag="VIG33_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8269708.1"
                     /translation="MNWQISKIEVSSFKAFKQVLLDLHNSSLLTLDGPNGFGKTSIFD
                     AIELLLTGQIKRIGNLFSKVMTRNKKNYEDNLFWNTRSGKKDLMIKIEFFNEERRFTL
                     ARYASSQTLTQKALNRADSFSQFSLYELPDFSSTDFSPENLRGNEYIEELFGENFREN
                     FCFLNYLEQGQNQLLLTRVDDRKDALGNLFNITDIAMEIENCKAVERSLNRFTKNDTR
                     KFQENELLAECESLRAMAQADLGSVEYKKLSTVDQQPGWDKENLFPTYSTEIFDLYRE
                     STRKLQELLPLKGAVRIRYQNERIETYIQQNMASLRSLAQFGSDTKKLSTLDNTKKEL
                     SQLTNAKAILQRGSTVITLAEAQALSGWEAERLKWFGELISVRNDLQQRNQANASVAA
                     ELARLKAKLLEEHGKLHPDDSTCPLCGADWKLHQAMLDAVEDRSQKIANTLGADGKAL
                     VDVTTRMNNELAPIAAHVQSLETTLSSGYNEALHLALESEKVRLDSISQLAEQLEANV
                     TTINYQYSANAEIVEARLRELIALIRAKKTVETEVLPDDWKQIVNSAFKVIEDFYIIE
                     QEDLTDKILYITIKANEARNN"
BASE COUNT          569 a          401 c          391 g          423 t
ORIGIN      
        1 cttattaagg aaatgattaa cgatgaattg gcaaatatca aaaattgaag tctctagctt
       61 caaagcgttc aagcaagtat tgctggacct tcacaattca tcactactga ctctggatgg
      121 acccaatgga ttcggcaaga caagtatttt cgatgccatt gaattgttac tcactggaca
      181 aataaaaagg atcggcaacc ttttctccaa ggtgatgact agaaacaaga agaattatga
      241 agataacctc ttttggaaca ctcggtcagg taagaaagac ttaatgatca agattgagtt
      301 cttcaacgaa gagcgcaggt ttacgttggc aaggtatgca tcttctcaaa ccttgacgca
      361 aaaagcactg aatcgtgcgg atagcttttc gcagttcagc ctatacgaac taccagactt
      421 ttcttcaact gacttttctc cagagaactt gcgcggtaat gaatacattg aggagctctt
      481 cggcgaaaat ttcagggaga acttctgttt cctaaactac cttgagcaag gacagaatca
      541 gttattgctt acaagagtag acgacagaaa agatgcctta ggtaacttat tcaatatcac
      601 tgacatcgcg atggaaattg agaactgtaa agcagttgaa cgcagtctta ataggtttac
      661 caaaaatgat acgcgcaaat tccaagaaaa cgagttgctc gccgagtgtg aatcgcttag
      721 agccatggcg caagctgacc tcggtagcgt tgagtacaaa aagctgtcca ccgttgacca
      781 gcagccagga tgggataaag agaatctctt ccccacttac tcaaccgaaa tcttcgattt
      841 ataccgtgaa tcgacacgaa agcttcagga gcttcttccc ctaaaaggcg ctgttcgaat
      901 tagatatcag aatgagcgca ttgaaaccta tattcagcag aatatggctt cgctacgaag
      961 cctggctcaa tttggctctg atacaaaaaa gctaagcact cttgataaca ccaaaaaaga
     1021 gctgtctcaa ctaacgaacg ccaaggcaat ccttcaacga ggctcgaccg tcatcaccct
     1081 tgcagaggcc caagctctat ctggttggga agccgaaaga ttgaagtggt ttggagagct
     1141 aatctccgta cgtaacgacc tccagcaaag gaaccaagca aatgccagtg ttgcggctga
     1201 actggctcgc ttgaaggcta aactgcttga agaacacggc aagcttcacc ccgatgattc
     1261 aacttgtcca ctctgcggag ccgactggaa attgcatcaa gccatgctgg atgcagtcga
     1321 ggacagatca caaaaaatcg ccaatacgtt gggtgctgat gggaaggctc tcgtcgacgt
     1381 gacaactcgg atgaacaacg aactggcacc aatagctgcc cacgtacagt ccctcgaaac
     1441 gactctatcg agtggctaca acgaggctct tcaccttgct ctagaaagtg aaaaagttcg
     1501 cttggatagt atttcacagt tggctgaaca acttgaagcc aatgttacaa cgatcaacta
     1561 ccagtattca gcaaatgcag aaattgttga agcgcgcctg cgagagctga tcgcgttgat
     1621 acgagcaaaa aaaacagtcg aaaccgaggt actgcccgat gattggaagc agattgtcaa
     1681 ttctgccttt aaagttattg aagacttcta catcatcgaa caggaagatc tgaccgacaa
     1741 aattttgtac attacgatca aagctaatga agcacgaaat aaca
//
LOCUS       DATBTE010000058         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2328707, whole genome shotgun sequence.
ACCESSION   DATBTE010000058 DATBTE010000000
VERSION     DATBTE010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2328707"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..810)
                     /locus_tag="VIG33_02390"
     CDS             complement(172..810)
                     /locus_tag="VIG33_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269709.1"
                     /translation="MNKAFTLTLLSILIHQTANAGWFVFNNKPKNEKQFIGTCSMGGS
                     FVKFGATMMETDPQGCKVDKENIHSIICMSDGGSSISTWFETQEECNSFRKNLQNKLV
                     KNSSSISKSSAKFGWVFTKLKSSQAFERFKPCEKVNANFEGHLKNESDMWSGRGAVCE
                     ASSFYDDHNSVFECKYPNLGDLTRYYFWHESKDKCESRLKMVKEKWKNEKLD"
     gene            complement(812..1093)
                     /locus_tag="VIG33_02395"
     CDS             complement(812..1093)
                     /locus_tag="VIG33_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8269710.1"
                     /translation="MKTKKMTFNKLSQASGVPISTIKTWSAGVEPKSLSHARKVARAL
                     DVSVEYLVFGESSVQAPTMSELLTTQLFSGWCKVTIEMPVTSPLLKKKE"
     gene            1344..>1525
                     /locus_tag="VIG33_02400"
     CDS             1344..>1525
                     /locus_tag="VIG33_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269711.1"
                     /translation="MFKRRYRSKKREAEALGSLIFVGIIFGVLSFVYHVAIEIGKTLL
                     EFLKTNNGKITAGVVLV"
BASE COUNT          444 a          296 c          293 g          492 t
ORIGIN      
        1 taaatggctc ggattctttt agccaatcgg ggcgagacga caattcatca attcgagaaa
       61 cgacacggtc cggttcgatc tcagctttgg ttggattttt tactgcggaa ctgcagccag
      121 taataagtag tgaaaaaatt aacatatact tcatgaaaca ctcctctttt attagtctaa
      181 tttttcattt ttccatttct ctttaaccat tttcaaacgt gattcacact tatctttgct
      241 ttcgtgccaa aaataatatc gtgtcagatc tcccaaattt ggatatttac attcgaaaac
      301 tgagttgtga tcgtcataaa atgatgaggc ctcgcagaca gcgcctctac ctgaccacat
      361 atcagattca ttttttaagt gaccctcaaa gtttgcattt accttttcac aaggtttaaa
      421 tctttcgaaa gcctgagatg attttagttt tgtaaaaacc catccaaatt tagccgaaga
      481 ttttgatatg gaagagctat ttttaaccaa tttattctgg agattctttc gaaagctatt
      541 gcattcttct tgagtttcaa accatgtact tattgaagag ccaccatcac tcatacagat
      601 tattgaatgg atattttcct tatcgacttt acacccctgc gggtcagtct ccatcattgt
      661 cgctccgaat tttacgaaag atccacccat tgaacacgta ccaatgaact gtttttcgtt
      721 ttttggttta ttattaaata caaaccagcc ggcatttgcg gtttggtgga ttaatattga
      781 tagtagagtc aacgtaaagg ctttattcat tttattcctt ctttttcaag agaggtgagg
      841 tcactggcat ttcgattgtt accttgcacc agccactaaa cagttgagtc gttaataatt
      901 cactcatggt tggagcctgt actgaggatt caccaaatac aagatactca acagaaacat
      961 ccaaagcgcg cgcaaccttc ctggcatgag acaaggactt cggctccacg cctgcgctcc
     1021 aggttttaat tgtagaaata ggaacaccag aagcctgact caatttattg aaagtcatct
     1081 tcttagtttt cataaggtgt tttagaatgg gaccaatttt tacttcatcg ctcattacaa
     1141 ataattatcg tctataaaag tatgtttgat gagctaaaga cgattccatg aaccgtggta
     1201 tgtgaggacg accattggtc aattgttgtt ttcgagagag agtggcacca tcagggctaa
     1261 ggtctgagta caggtaagta gaccgcgcag gtcattgcaa agctattaat atttgtcgat
     1321 ttagagccga taaagaggag catatgttta agcgaagata tcgaagcaaa aaacgagaag
     1381 cagaggccct tggatcgttg atctttgtgg gtattatttt tggtgttctt agtttcgttt
     1441 atcatgtagc aattgaaatt ggaaaaacac tccttgaatt cttgaaaacc aataatggaa
     1501 agattacggc aggagtcgtc ctagt
//
LOCUS       DATBTE010000059         1814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1372303, whole genome shotgun sequence.
ACCESSION   DATBTE010000059 DATBTE010000000
VERSION     DATBTE010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372303"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="VIG33_02405"
     CDS             complement(<1..1169)
                     /locus_tag="VIG33_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269712.1"
                     /translation="MRSFELSVFILTLMLFFSPAFATVSAARSQQQDFMRQFQTSRVL
                     FPDENSPYAARTDGQYSAPLRFLLQDFLAWTPSGEGRALSSKCATELWSARFQDARVH
                     NSIQLQGALIQKYLKDCGEEFATGHDSMFSNFTKMMTMKYEPHAHPFLHRVVFSLPGN
                     IKLKGLLGLKGDFKKRPLVVIRLGIFSNVEEFMPERAWLMMLFEQAPFNVLMLENMSG
                     TDFVADNSRFSFGGYDEAIQNILVGQILKDPNEPLSKLVESLHLFGVSLGGHGVLYAS
                     LLNDLNSPKGQPLYQSFLGMCPVVNIEENMKNLTSEKPFSFLVDLWSQKRLAGLAKKM
                     TAVENHESFAFLPKMISEVVRTYHGGLSYVSSVKLPAGVEDGPQFFQLNDFWKNY"
     gene            complement(1211..1753)
                     /locus_tag="VIG33_02410"
     CDS             complement(1211..1753)
                     /locus_tag="VIG33_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8269713.1"
                     /translation="MSWEFIQASVELAAEQSKEISELVNSAYRGEGAKKGWTTEADLL
                     SGQRTDVASIRHLIEKDNSVMLLAENENSGKLEGCVHLEKHDEKCYLGMLTVKPTLQR
                     QGLGAMLLEEAEAFAQFWDCTSIYMTVIAQRTELIQWYEKHGYKNSGENKSFPYGDER
                     FGIPKLQDLYFVVLEKKMAE"
BASE COUNT          551 a          436 c          347 g          480 t
ORIGIN      
        1 ttatagtttt tccaaaaatc attcagttga aaaaattgag ggccgtcttc aacccctgct
       61 ggcaatttta cagaggaaac ataggaaagt cctccatgat aagtacgtac cacttcagaa
      121 atcatttttg gaagaaatgc aaagctctca tgattttcca cagcggtcat tttttttgcg
      181 agtcctgcca gacgtttctg actccataaa tcgacgagaa aagaaaaagg cttctcagaa
      241 gtcaaattct tcatattttc ttcaatgttc acgactggac acattcctag gaaactttga
      301 tagagaggtt gcccttttgg agaattcaaa tcattcaata aagaagcata gaggactcca
      361 tgaccgccaa ggctgactcc aaataagtga agactctcca ccaatttact gagcggttca
      421 ttgggatcct ttaaaatttg acctacgaga atattctgaa ttgcctcatc atagccgcca
      481 aaagagaaac gactgttatc cgcgacaaag tccgttccag acatgttctc caacatcaag
      541 acattaaagg gagcttgttc aaaaagcatc atcagccaag cacgttcagg cataaactct
      601 tcaacattcg aaaaaatccc cagacgaatc actaccagag gacgtttctt aaaatccccc
      661 ttaagcccca gcagaccctt taacttaata tttcctggaa ggctaaaaac cacacgatga
      721 agaaaaggat gggcatgggg ctcatacttc attgtcatca tcttcgtaaa attcgagaac
      781 atactatcgt ggccagtggc aaactcttca ccgcaatctt ttaagtactt ttggatgagg
      841 gctccctgaa gctgaattga attatgaacg cgggcatctt ggaaacgcgc actccacaat
      901 tcggttgcac acttactaga gagcgcgcgg ccctcaccac ttggagtcca ggcaagaaag
      961 tcttggagta aaaaacgaag cggcgctgag tattgaccgt ctgtccgagc ggcatacgga
     1021 gaattttcat ccggaaacaa aacgcgagaa gtttgaaact gccgcatgaa atcttgttgt
     1081 tgactccggg ctgcagagac cgtcgcaaaa gcgggcgaaa aaaacagcat taaagtgagt
     1141 ataaaaacgc ttagttcaaa ggaacgcatt aaaaatcctt ttgctagtag ccatcaacta
     1201 tgaaaaacac ctactccgcc atcttttttt caagaactac aaagtataaa tcctgaagct
     1261 ttggaatgcc aaacctctca tctccataag gaaaggattt attttccccc gaatttttat
     1321 atccatgttt ttcataccat tgaatgagtt ccgtcctttg agcaatgacc gtcatataga
     1381 tgctagtgca gtcccaaaac tgagcaaagg cctctgcctc ttccaacagc atcgctccca
     1441 agccttggcg ctgtaaagta ggttttacgg taagcatgcc taaataacat ttttcatcgt
     1501 gtttttcgag atgaacacag ccctccaatt tgccgctatt ttcattttcc gcaagcaaca
     1561 tgacggaatt gtctttctca ataagatgac gaatgcttgc aacatccgtt cgttggccac
     1621 tcaaaagatc cgcctccgtg gtccaaccct ttttagcgcc ttcgccacga taagcggaat
     1681 tgaccaactc actaatctct ttagattgct ccgctgccaa ctcaacactt gcttgaataa
     1741 attcccagct cataaaggct ctccctgatt gagagttatc ctatcaggat tgactgatcg
     1801 gggcaatttg aagt
//
LOCUS       DATBTE010000060        11322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_552865, whole genome shotgun sequence.
ACCESSION   DATBTE010000060 DATBTE010000000
VERSION     DATBTE010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 11322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11322
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552865"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VIG33_02415"
     CDS             complement(<1..322)
                     /locus_tag="VIG33_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEY8269714.1"
                     /translation="MARIKGKNTKPEKLVRSLIYSLGYRFRIHFKGLPGKPDIVFPGR
                     RKVIFIHGCFWHGHEGCKRSKIPSTNTAFWETKINKNRSRDALVTTELANMGWQVLTL
                     WQCQI"
     gene            complement(462..2255)
                     /locus_tag="VIG33_02420"
     CDS             complement(462..2255)
                     /locus_tag="VIG33_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8269715.1"
                     /translation="MGIYMVDVESIKNEFQNNPSYKPYITSIVFHYYKNLTPDSELKF
                     EFPLTLLIGKNGTNKSSILHALYGCPEGKSTGEYWFSTHIDPIDNTEGKRAAYFYRYT
                     VKDLLIQPEVLKSRMRRTDDPDYWETARPKTEYGMLKIPPVEKSMLPYLSKTHRRWNP
                     IHKAVHYLDFRAEISAFDKAFYKDSNSESRAIRKKRIRNHSKALKVAIEEELKTKIYF
                     KKNRIFSNNKFTRKQREIVNNILATEYSEIVYIEHDFYLSGSFSVYIKKNDENNNYSE
                     AFAGSGETSIVRLVYALENSEEGALILLDEPETSLHIEAQSRLRDYLLHKIREKHLQV
                     IISTHSPYFAKGLPDCAIKILDVDSSTKKINIINEAPAEDSSFYLGYQRNVAEKTNII
                     VEDRLAKAIVKKVYSEILSPNIRDKIALQIQPGGATQMLRRAVIEMSKPYSNEWFLFD
                     GDQKLHSQIPDPSDIPESKNNKLESVVAKVFGHCPDFPSLSNSPERIQHMRRFLAFAK
                     ERFLYLPFQTPEEFIVQNNLDEFKKSQNKPPKDVLRDYCHNSLGSTGDVNGDGIFTIQ
                     RFLLSKISIKHDAFQEIKQQIGGICKSCFSE"
     gene            complement(2452..2787)
                     /locus_tag="VIG33_02425"
     CDS             complement(2452..2787)
                     /locus_tag="VIG33_02425"
                     /inference="COORDINATES: protein motif:HMM:NF024013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3768 domain-containing protein"
                     /protein_id="HEY8269716.1"
                     /translation="MSQLDKSRRIAALNDALRSTYNSGTLVLTAGIQAHPEEVQVGIL
                     YAVRNFNAFTPDNDPHGEHDFGSLTHQGIHVFWKIDYYDQNMVFHSEDPADPASTQGV
                     LTIMLATEY"
     gene            2950..3198
                     /locus_tag="VIG33_02430"
     CDS             2950..3198
                     /locus_tag="VIG33_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269717.1"
                     /translation="MLERLSSQEITELVIIVLLSYHMREADRLESIIQETGQKNRWHE
                     FWPQFQAQAENDEEALRRFTQFVMAQLDGGEIHLPRTS"
     gene            3253..3525
                     /locus_tag="VIG33_02435"
     CDS             3253..3525
                     /locus_tag="VIG33_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269718.1"
                     /translation="MKYESIKLLFQQDKYVQITSVLMLLVFGYSFYHLSTSLVKSMES
                     EHRKSIYEIHSGNVTVRNDVFSDKSWILNKCNEPAGYCWEPIPSKG"
     gene            complement(3551..3817)
                     /locus_tag="VIG33_02440"
     CDS             complement(3551..3817)
                     /locus_tag="VIG33_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269719.1"
                     /translation="MSQASFYDSLSSLEKTYWHKLSEVFQLVAAQKPEKGIETLLLRA
                     RKAAEQNGTTLAQELETVFQGAQERTLRRMELLGACQLPKPSDK"
     gene            complement(3827..4126)
                     /locus_tag="VIG33_02445"
     CDS             complement(3827..4126)
                     /locus_tag="VIG33_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HEY8269720.1"
                     /translation="MHVIVDLCVVPLGVGVSVSKYVVECEEILNEAGLKTHLHAYGTN
                     IEGEWDEVFAAIQRCHERIHEMGAPRISTTIKLGTRTDKQQTMEDKIRSVEEKLG"
     gene            complement(4162..4395)
                     /locus_tag="VIG33_02450"
     CDS             complement(4162..4395)
                     /locus_tag="VIG33_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8269721.1"
                     /translation="MERDETYVKALGQAVRNLRKAKGYSQESFADAVGLHRTYMGAIE
                     RGEQNLTVKNIVKVADTLGLKPSKLLAEAEKLL"
     gene            4519..4779
                     /locus_tag="VIG33_02455"
     CDS             4519..4779
                     /locus_tag="VIG33_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269722.1"
                     /translation="MKDIVFNLAALALMYFAWTTLCKYSHYKQIAEQHYREQDDKFGV
                     QRPVKQETMWDTAVIILQIIGFVSVVILVGTGLVSCVYGHSS"
     gene            4797..5120
                     /locus_tag="VIG33_02460"
     CDS             4797..5120
                     /locus_tag="VIG33_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269723.1"
                     /translation="MKRKSLMKNLMTLIPILLLAPPVMAQAPTEAWQDAQRACAIKAQ
                     ALTGNIKYEIEFEFNNWIECQAKAVSQNDKDCSANFRLLQKVIESNVKAAEEYLKDCE
                     VPCSQ"
     gene            complement(5817..7805)
                     /locus_tag="VIG33_02465"
     CDS             complement(5817..7805)
                     /locus_tag="VIG33_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HEY8269724.1"
                     /translation="MDISFNPHIINKPEHFPSEHAHKFEPMDLSLDQVIEKVTQGQAL
                     CVQFNQTRKKENFISAGYVCLDFDGKMSLEAARNHPFIQNHAGFIYTTASHGKEGKGD
                     CFRAFLPLSEPITSIQEYEALLESLRDIFPDLDEKCLDGTHLFYGNTEAEIIRFNQEI
                     DRLTLSVLISGAITAILKSQDPIGEGERNASLASIGGSLRSVGFEKEEITEWLLKVNE
                     QRCTPPLPEREVKNIAKSVARYAADDEKKKPSANAIAEKIIAATTIKTNQFGNPYEYQ
                     EGIWKPLTDAKLVSMALQAESKADSNRNRRNEMVSYIKAATFHGEIHWRQIQSWHIPL
                     RNGVFDLVDKSLLDHRPEYYLETVLPHDYVPDAKAPRWEAALQEWLADEPAKIQALQM
                     FFGYVLMSHAKYKKALLGYGESNTGKSQVAKVLEALVGSHNMCSISLNKMGNVRNIAP
                     IKGKMLNLVTELPEFHQLAEGGFKQLVSTEDPIMIDPKYLQPEMYVPFCKHVVFTNTL
                     PQIRDLSDAVFNRLLIIEFNRVISEDRQDKSLFEKLKEEMPGIVQWAIDGAIMLHEAK
                     GIFIEPESSKELLDEYRRKQNPVCEWAEQHLQYRDGAITTFEALWSDFRAYYKGKDVN
                     RDRFSKLLKKAGFETERNRVEGITTTFIPNYTVANPPY"
     gene            complement(7834..8073)
                     /locus_tag="VIG33_02470"
     CDS             complement(7834..8073)
                     /locus_tag="VIG33_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269725.1"
                     /translation="MPKEPYIYPDGRMNPPNTSLYIGLSIKRLAQMRCDGTGPHFVKV
                     GGRVFYFKEDVDNWINQSGRAASTAEIQFKKLSVS"
     gene            8247..9116
                     /locus_tag="VIG33_02475"
     CDS             8247..9116
                     /locus_tag="VIG33_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269726.1"
                     /translation="MSYNFSYLSRTLEDWYEEKSQEHGQSFIHTENPIEYLLSEHQDL
                     YKKLDMSQKRFEKVFLKPYYDLIDKGCFDFETMKSITGVLSQIVGKTVIIQRDLHVEP
                     FSLTIETTQDRYAKALDWVCVQIMELNLQGLELPENVYDLVDEPVLQCRYCGKLDTDA
                     KGREFNRKRKYCHSLKCTESKSVNPEHHIGCCFGEWARKKKLLVQALKRHYKGKEKGK
                     EYFERTCNELYEHALTLSEPVRTHQTLLRELLASLPTKDEIQREEARQLFKKYDGISL
                     HQILKALRQGDHV"
     gene            9109..10248
                     /locus_tag="VIG33_02480"
     CDS             9109..10248
                     /locus_tag="VIG33_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY8269727.1"
                     /translation="MSNKYIKLTREAMKTLKPGQYICEQNIKYTKLDNGDGLFSVYGR
                     ANGRRFHRNVGKESQGITRMQAEEVFQKLMQDTKADRFNLPKGRKYEQTFQEMAKRYL
                     ERLNETGGKNIDRKKQQLDAKLIPFFKHQPLSKITTFDVERFKKAMLDQGNKPATVNR
                     WLCTLSHLINKALEWGWIEHKPCVIKRLKEDNARITYLTAEQCQRVLEEAKNDTYPII
                     YPFIKIALETSMRRMEILSIRLEDIDFDRCMIYIPKAKAGAREQPMTKHLAEYLKEYV
                     QMVDKEQPWLFPSNRSKSGHYETIEHPWRRVLKAAGLDGYEIVRHTLRHTAITHLVQA
                     GVDLPTVMRISGHKSLQMVQRYSHQNGAHIEAAMEKLEKRYLTSS"
     gene            10678..>11322
                     /locus_tag="VIG33_02485"
     CDS             10678..>11322
                     /locus_tag="VIG33_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269728.1"
                     /translation="MNPDKEPNDIYITWKQLVRESRIKHCIYPITKECSETVIKAHSI
                     QRNRYLSHIADHGEVYMILPQLGEDFTVKAEKVGIKAATVFTGFCGYHDNKIFEPIEI
                     EPYKNTPLQNFLFAYRAFAFAYHRKLEAVQFALNHLKKKPDLVNHTMIQLQNEGYKLA
                     IRDITYAKEKFDAAFGNSSKEINTFVLEIPKECYIAACGSFFPEYDLQGQLLVDL"
BASE COUNT         3075 a         2521 c         2549 g         3177 t
ORIGIN      
        1 taatctggca ttgccatagg gtcaacactt gccaacccat atttgctaat tctgtagtaa
       61 caagcgcatc tcttgacctg tttttattaa tttttgtttc ccagaaagca gtatttgtag
      121 atggaatttt ggaccttttg cacccttcat gcccatgcca aaaacaacca tgaataaata
      181 tcaccttcct tcttcctgga aaaactatat cgggcttgcc agggaggccc ttaaaatgaa
      241 ttctaaacct atatccaagt gaatatatta aagacctaac tagtttttct ggcttcgtat
      301 ttttaccttt gatacgagcc attatctggc ttcgcttctt tttagtataa agatccgcca
      361 tataaaaagc cttttaagtt cagtgatgtg gtatctgaac ggtgcatttg aaaaaagttc
      421 ccctgaatgt ttaagcatca ttctttgagg actataggtc tctattcact aaagcacgat
      481 ttgcaaattc ctccaatttg ttgttttatt tcctggaaag cgtcatgctt aatactaatc
      541 tttgatagta aaaagcgttg aatggtaaaa ataccatccc cattaacatc accggtactt
      601 ccaaggctgt tatgacaata gtctctgagg acatcttttg gaggcttgtt ttgggatttt
      661 ttaaattcat ctaaattatt ttgcactata aattcttcag gtgtttgaaa aggaagatat
      721 aaaaatcgtt cttttgcaaa agctaaaaat cttctcatat gctgaattct ttccggagag
      781 ttcgagagag atggaaaatc ggggcaatgc ccgaatactt tggcaactac agactctaat
      841 ttgttgtttt ttgactcagg gatatctgaa ggatcgggaa tctgactatg taatttttgg
      901 tccccatcaa acagaaacca ctcatttgaa tatggcttgc tcatttcaat tactgcacgg
      961 cgaagcatct gagttgctcc gcctggttga atctgaagag caattttgtc cctaatatta
     1021 ggtgaaagaa tttcgctata tacttttttg acaatggctt tcgccaaacg atcctcaaca
     1081 ataatattag ttttttcggc tacattcctt tgatacccaa gataaaatga actatcttct
     1141 gccggtgcct cattaataat attgatcttt ttagtagaac tatctacatc taatatttta
     1201 atagcgcaat ctggtaatcc ttttgcaaaa tagggagagt gggtagagat aattacttgc
     1261 aagtgtttct cacgaatctt gtgaagtaaa tagtcacgca accgactttg ggcttcgata
     1321 tgtagtgatg tttcaggttc atctagtaag atcaaggcgc cttcttcaga gttttcaaga
     1381 gcgtatacaa gccgtacgat gctggtttct ccgctaccgg caaaggcttc agaataatta
     1441 ttattctcat cattcttttt tatataaact gaaaagcttc ctgataagta aaaatcatgt
     1501 tcaatataga caatttctga atattctgtg gcaaggatat tgttgacgat ctctctttgc
     1561 ttcctagtaa acttattatt gctgaatatt ctattttttt tgaaatagat tttagttttc
     1621 aattcctctt cgatggctac ctttaaagct ttagaatggt ttctaatcct ctttttacgg
     1681 attgctcttg attcagaatt actatcttta taaaaagctt tatcgaaggc acttatctca
     1741 gccctaaagt ctaggtaatg tactgcttta tggataggat tccaacgcct gtgcgtttta
     1801 cttaaatatg gcagcatgct tttttcaaca ggaggaattt tgagcatgcc atattcggtt
     1861 ttgggacgag cagtttccca atagtcgggg tcatccgttc ttctcattct ggatttcaaa
     1921 acttctggct gaataagcaa atccttaaca gtataacgat agaaataagc agccctcttt
     1981 ccttcagtgt tatcaattgg atctatgtga gttgagaacc aatactctcc tgtcgatttc
     2041 ccttcagggc aaccgtaaag agcatgtaaa atagaactct tgtttgtgcc attctttcca
     2101 ataagaagag ttagagggaa ctcgaattta agctccgaat caggagttaa gtttttataa
     2161 taatgaaata caattgaagt tatataaggt ttataacttg gattattttg aaactcattc
     2221 ttaatacttt ccacatctac catatagata cccatcttct atgctcttaa atagtagaag
     2281 atatcttgat tttaataaag tcaaaataaa agccggttcc gataactgcg attatgctcc
     2341 cgattccttc tcacccattt gggtgagaga atagtttgca gaggcttctg aacgattgcg
     2401 gcagcctgtg ctctacttcg gatgttgtat tatccccctt aaaccctgtt tttagtactc
     2461 agtcgccaac atgatggtta aaaccccttg agtgcttgcc ggatcggcag ggtcttcgct
     2521 atgaaacacc atgttctggt cgtagtaatc aattttccaa aagacatgaa tgccttgatg
     2581 cgttagtgaa ccaaaatcat gctcgccatg cgggtcgtta tctggggtga acgcattaaa
     2641 gttacggacg gcatacagaa tgccgacctg cacctcttcg ggatgcgcct ggatgcctgc
     2701 ggtcagaacc agggttccgc tattgtaggt cgagcgaagg gcatcattca atgctgcgat
     2761 ccggcggctt ttatccaact gagacaagac aagactccta ttgggcaggt gacatcgctg
     2821 tcattctgtg ccaaaaggag ctggccctgt gatttgtgag gggaaaatat tacaaaagcc
     2881 tacaaatatt gctctggccg tccatacagc caataatcct cttgatttat ctgggttagg
     2941 agggttgtca tgctggaacg gctatcatct caggagataa cagaactggt catcattgta
     3001 ttgctttcct accatatgcg agaggctgac cggttggagt ccatcatcca agagaccggg
     3061 caaaagaacc gatggcatga gttctggccc caattccaag cgcaggcgga aaacgatgag
     3121 gaggctctcc gccgctttac ccaatttgtt atggctcaac tggatggcgg cgagatccac
     3181 ttgcctagga ccagttgaaa ttttcagaat cggactatga ttccaaaagt aagctgaata
     3241 cgaggtgttg gtatgaaata cgaatctatc aagcttctat ttcaacaaga caagtatgtc
     3301 cagattacca gtgtgttgat gctgcttgta tttggatact ccttctacca tctgtctaca
     3361 tctttggtga aaagcatgga aagtgagcat cgtaagagta tttacgaaat tcattcaggg
     3421 aatgtcaccg tcagaaacga tgtcttttcc gacaagagtt ggatcttgaa taaatgtaat
     3481 gagccagcgg gttactgctg ggagccgatt cccagcaaag gataatggcg ccgttgtatg
     3541 acggcaaggt ttacttatca gagggcttcg gaagctgaca agcgcctaac agttccattc
     3601 tccgcagcgt tctttcctga gcaccctgaa acaccgtttc gagttcctgt gccagagttg
     3661 ttccattttg ttccgcagcc ttcctagctc tgaggagcaa ggtctcaatg cctttttcgg
     3721 gcttttgtgc cgccaccagt tgaaacacct ctgacagctt gtgccagtag gtcttctcca
     3781 gtgaagacag agagtcataa aagctggctt ggctcatggg gtatccctaa ccgagtttct
     3841 cttcgacact gcgaatcttg tcttccatcg tctgttgttt atcggtccga gtgcccagct
     3901 tgatggtcgt agagatccga ggcgccccca tctcgtggat tctttcatga cagcgttgaa
     3961 tggcggcaaa cacctcgtcc cattcccctt cgatattggt gccataggcg tgcaggtggg
     4021 tcttcagccc ggcctcattc aaaatctcct cgcattcgac cacgtacttg gatacggaaa
     4081 cgcccacccc caagggaacg acgcataagt ctacgatcac gtgcatggga aagcctctct
     4141 gttgaactgt tgccaatgaa gctataacag tttctctgct tcggcaagta gtttggaagg
     4201 cttcagaccc agggtgtcag ccactttaac gatgtttttg acggtcaggt tctgctctcc
     4261 ccgttcaata gcccccatat aggtccgatg cagccccacc gcgtcggcaa agctttcctg
     4321 gctgtatccc ttggctttcc gtaggtttct gacggcctgt cctaacgctt taacgtatgt
     4381 ttcgtctctt tccatatgcc gagaatacgg ctagacatac taaagagcta cagtatataa
     4441 atagacatcc tttaactagc atttcgctgt agaatggcct tatacagctt ccccatcagg
     4501 gctttagcct ggagtcatat gaaagatata gtcttcaacc ttgccgcact ggctttgatg
     4561 tattttgcgt ggacaactct atgcaaatat tcccattaca aacagattgc cgagcaacat
     4621 tatcgagaac aagatgacaa gtttggagta cagcgaccag ttaagcagga aactatgtgg
     4681 gatacggcag taataatttt acaaataatt gggtttgtca gtgttgttat tttagtaggc
     4741 accggacttg tgtcttgcgt atatggtcat tcgtcttgag tatgagtgaa ttgagaatga
     4801 aaagaaaatc ccttatgaaa aacctaatga cattgatacc catactactg cttgctcccc
     4861 cagtcatggc acaagcccca actgaggcct ggcaagatgc ccaaagagcc tgtgccatta
     4921 aagcacaagc cctaactggt aatatcaaat atgagattga gttcgagttc aacaactgga
     4981 tcgaatgcca agcaaaagct gtgagccaaa atgataaaga ctgttccgct aacttcagac
     5041 tgctccagaa ggtaattgag agcaatgtta aagcggcgga ggaatatttg aaagattgcg
     5101 aagttccatg ttcacagtga attggatgcc ctctcccgat ccagggcgtg gttttggcgg
     5161 gaacgaccgt aaaatctggt ttctggggtt tcggagaata aattatttca atgtgcccct
     5221 tatatcgata tccccctgcg cttaaacgcc tgttgttgag tcgccaggat ggttctggcg
     5281 gggtctggtg gagtgtcgag gggacggggt gacataaagg gatagcctag gtgacggcgc
     5341 caagcggtac agtgtttcaa gttctgatct ggggctcgtt aattcatttg ggtttttggc
     5401 gcgtgaaagt cgcgtcttat aggatggggt ataggtcgat aatcgcctgg agtgctgggg
     5461 tgggttggag gaccgtggac ggtggatctt gaatagcggt cactcatctg ttggaaacaa
     5521 tgagaggtta ccaatgcgct ttctggtctc taggttcttg cttcataagc cccaacgtca
     5581 gatttatgag tcacctgaac cagatttgtt tgctctacgc tcactcatgg aatttaaaag
     5641 gttaaaaata aaaaatataa aaaaagatat caccgaagaa aaatataaaa aatattttta
     5701 tccatttttc acttccgtga tagagcagtg tacatttgct ctatgctcac tcaggtaagc
     5761 caaaaagttc acttggctaa gtgaactttt ttgacccaag atcaggcagg cttgaatcag
     5821 tatggcggat tggcaacggt ataattgggg atgaaggtcg tggtaattcc ctccactcgg
     5881 ttccgctcgg tctcaaaacc ggctttcttg aggagctttg aaaaccgatc tcgattgaca
     5941 tccttccctt tgtagtaagc acggaagtca gaccaaaggg cttcgaaggt tgttatcgct
     6001 ccatcccggt actgaagatg ctgttcggcc cattcgcata ccgggttctg cttccgacga
     6061 tattcgtcca gaagctcctt ggagctttcc ggctcgataa agatgccttt ggcttcgtgc
     6121 agcatgatgg ctccatcgat agcccactgg acgatcccag gcatctcctc cttcaacttc
     6181 tcaaacaggc tcttgtcctg cctgtcttca gaaatgaccc ggttgaattc gataatcaac
     6241 aaccggttga agacggcatc gctaagatcc cggatttgcg gcaacgtatt ggtgaagacc
     6301 acatgcttgc agaaaggaac gtacatctcc ggttgaagat acttcggatc tatcatgatg
     6361 ggatcttcgg tgctgaccaa ctgcttgaag ccaccttcgg ccaactgatg gaactccggc
     6421 aattccgtta ccaagttcag catctttccc ttgatgggtg cgatattgcg gacatttccc
     6481 atcttgttca gcgagatact gcacatgttg tgggaaccga ccagagcttc cagcaccttg
     6541 gccacttggg acttgccggt gtttgactcc ccatagccga gcagcgcttt tttgtacttg
     6601 gcgtgggaca tcagaacata accgaaaaac atctgaagtg cttgtatttt cgccggttca
     6661 tcggccagcc actcctgaag cgcggcttcc caacgtggcg ctttggcgtc tggcacataa
     6721 tcatggggca agacagtttc cagataatac tccgggcggt gatccaagag gcttttgtca
     6781 accaggtcaa acactccgtt tcgcaaggga atatgccagg actgaatttg ccgccagtgg
     6841 atttcgccat ggaacgtggc ggccttaata tagctgacca tctcgtttcg acgattccgg
     6901 ttgctgtcgg ccttggattc agcctgtaaa gccatggaaa ccagctttgc gtcggtcaag
     6961 ggtttccaaa tcccttcctg gtattcatag gggtttccga actggttggt tttaatggtg
     7021 gtggctgcga tgattttttc ggctatggca tttgccgagg gtttcttttt ttcgtcatca
     7081 gcagcgtagc gcgccacgct tttggcaatg ttcttcacct cgcgctctgg caatggggga
     7141 gtacagcgct gctcattcac tttgagcaac cattccgtta tctcctcctt ttcaaagccc
     7201 acagagcgta gagagccacc gatactggca aggctggcat tacgctcacc ttccccaata
     7261 gggtcctgag acttgaggat cgcggtaatc gcaccggaga tcagcaccga aagggtcaac
     7321 ctatcgattt cctgattgaa gcggatgatc tcggcttcgg tgtttccgta gaacagatgg
     7381 gtcccatcca ggcacttttc atccaggtcc gggaagatat ctcgcaggga ttccagaagc
     7441 gcctcatact cctgaatgct ggtaatgggc tcactcagag gcaggaacgc cctgaagcag
     7501 tcgcctttac cttccttgcc gtgcgaagcc gtggtataga tgaaacccgc atggttctgg
     7561 ataaacggat gattcctggc cgcctccaaa gacattttgc catcaaagtc caggcaaaca
     7621 tacccggcgc ttatgaaatt ctctttcttg cgggtctggt tgaactggac gcatagcgcc
     7681 tgtccttggg tcactttctc aatgacctga tccagtgaaa ggtccatggg ctcgaacttg
     7741 tgcgcgtgct ccgaagggaa gtgctccggt ttattgatga tatgagggtt gaatgaaata
     7801 tccatattat tttccttttc ttgttgcgac tagttatgat acggacaatt tcttgaattg
     7861 aatttcagca gtgctcgctg ctcgaccgga ttggttaatc cagttgtcaa cgtcttcctt
     7921 gaaatagaag acccggccac caactttcac gaaatgagga ccggtgccgt cacatctcat
     7981 ttgcgccaat cgcttgatgg ataggccgat atagagggat gtattcggcg ggttcatccg
     8041 gccatcgggg tatatatagg gctctttagg catgggtttc tcctttttga tgaatgtttc
     8101 ctacgaatag cctgaatgtt ctcggctatg cttagcaggt tattccaggg cagaaaccca
     8161 tagccctcat tatttgatga atgtattttt ctggactctt gacctataat cagagccatt
     8221 tggatctcga ccttggaatt tgaagaatga gttataactt tagttacctc tcccggactc
     8281 ttgaagattg gtatgaagag aaatctcaag agcacggtca gagctttatt cacactgaaa
     8341 atccaattga atacctcttg tctgagcatc aagaccttta taaaaagctg gatatgtcgc
     8401 aaaaaaggtt tgagaaggtt ttcctgaagc cttattacga tttgattgat aagggttgtt
     8461 ttgactttga aactatgaaa agcatcacag gagtcctgtc ccagattgtg ggaaagactg
     8521 tcattatcca aagggattta cacgttgaac cattctctct aaccattgaa acgacccagg
     8581 accgttatgc taaagcccta gactgggttt gtgtacagat tatggaattg aacctacaag
     8641 gtttagaact acccgagaat gtctatgacc tagtagatga gccagttcta caatgccggt
     8701 attgtggcaa gctcgataca gatgctaaag ggagagaatt caatcggaag cgaaaatact
     8761 gtcatagcct gaagtgtaca gaatctaaat ctgtgaatcc tgaacaccat attggttgtt
     8821 gctttggcga atgggccaga aagaaaaaac ttcttgtgca ggctttaaaa cgccattaca
     8881 aaggcaagga aaaagggaaa gaatattttg aaagaacctg taatgaactt tacgagcatg
     8941 ccttaaccct atctgagcct gtcaggactc atcagaccct attacgggaa ttgcttgctt
     9001 cattacccac gaaagatgag atacaacgtg aagaggctcg gcaactcttt aaaaaatatg
     9061 atggtatttc cttacatcaa atcttaaaag cccttaggca aggtgaccat gtctaataaa
     9121 tatataaaac ttacccgcga agccatgaaa accctaaagc cggggcagta catctgtgaa
     9181 caaaacatca aatacaccaa gttggataac ggggacgggc tgttctcagt ctatggcagg
     9241 gctaacggca gacgctttca ccgcaatgtg ggcaaagaat cgcaaggcat cacccggatg
     9301 caggccgagg aagtcttcca aaagctgatg caggacacca aggcggaccg cttcaaccta
     9361 cccaaaggcc ggaaatacga gcaaaccttc caggagatgg ccaaacggta tctagaacgg
     9421 ctcaatgaaa ccggcggcaa gaacatcgac cgcaaaaagc agcagttgga tgccaagctg
     9481 attcctttct tcaagcacca accactctct aagatcacca cctttgacgt ggagcgtttc
     9541 aagaaggcca tgctggacca gggtaacaaa ccggccactg tgaaccgctg gctatgcaca
     9601 ttgtcccatc tcattaacaa agccttggaa tggggctgga tcgagcataa accttgtgtc
     9661 atcaagcggt tgaaagaaga caatgcccgc atcacctatc tgacggcgga gcaatgccag
     9721 cgggttctgg aagaggccaa gaacgatact tatcctatca tctatccgtt catcaagatc
     9781 gccctggaaa cctcgatgcg ccgcatggag atactctcga ttcggctgga ggatatcgat
     9841 ttcgatcgat gcatgattta tatccccaag gcgaaagccg gggccagaga acagcccatg
     9901 accaagcatt tggccgagta cttgaaggaa tacgttcaga tggtggacaa ggagcagccc
     9961 tggctgttcc cgtcaaaccg ttcgaagtcc ggccattacg agactatcga acatccctgg
    10021 aggcgcgttc tgaaggccgc tgggctggac gggtatgaga tcgtccgcca taccctccgg
    10081 cacacagcca tcacgcacct cgtgcaagct ggggtggatc tccccaccgt catgcgcatc
    10141 agcggtcata aatcccttca gatggttcag cgctatagcc accagaatgg cgcccacatc
    10201 gaggcggcaa tggagaagtt ggaaaaacgg tacttgacta gctcataagt tagtcaaata
    10261 atccagtaat gccgctggat agtaagtttg acatcaacaa gttggagtta gggggtctat
    10321 attccctggc aaacttggct gatttgcaga atatacctgc catcaagact gctcaatcag
    10381 gggtaatccc tttccaaaac tgtatcttgc ttctacttac actagaaaaa tccaaatata
    10441 ggaatagggt cgacaaagat cagttaagaa tgttctttca ggagagtagt acacaagaat
    10501 cccctgccat caagcgattc ctaagtgctg atagggaagt actactgttc ttgcgtaaaa
    10561 agcgattgag tcaaggtaaa accatgccgt atcaatatat ggggcgttta gagctgaaag
    10621 aaatctttgg caataagcca gtggaaatta catggtgttt aatcgacttt aagaacaatg
    10681 aacccagata aagagccaaa tgatatttat attacctgga aacagcttgt cagggaaagc
    10741 cgcattaaac attgtatata tccaataact aaagagtgtt cggaaacagt aatcaaagct
    10801 cattctatac agagaaacag atatctgtca catatagcag accatgggga agtgtatatg
    10861 atacttcccc agctaggaga agactttacc gtaaaagcag aaaaggttgg aatcaaggct
    10921 gcaactgtat ttacgggctt ttgtggctat catgataaca aaatatttga gcctattgag
    10981 atagaacctt acaaaaatac accacttcag aatttcttat ttgcatatag agcatttgca
    11041 ttcgcttatc atagaaaact agaggcagtc caattcgccc taaatcacct taaaaagaaa
    11101 ccagatttag tgaatcatac tatgattcag ttgcaaaatg aagggtataa actcgccatt
    11161 agagatatta catacgcaaa agaaaaattt gatgccgcat ttggtaactc ttctaaggaa
    11221 attaatactt tcgtcttgga aatcccaaaa gaatgttata ttgctgcttg tggcagcttc
    11281 ttccccgagt atgatcttca gggtcagctc ctggtagacc tt
//
LOCUS       DATBTE010000061         1583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1850744, whole genome shotgun sequence.
ACCESSION   DATBTE010000061 DATBTE010000000
VERSION     DATBTE010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1850744"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..1367
                     /locus_tag="VIG33_02490"
     CDS             39..1367
                     /locus_tag="VIG33_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269729.1"
                     /translation="MHYHHEQKPDDWTEDKIQGMVFHFLIVKSLAKAMGGSDAVAKVE
                     SPLRTLNMPDDLREEILNPPLPLEKYENMAIEEILSKTSEEIWGAPFTDCGPTRIYRW
                     KALGISWFASTENRLSGLPYAEEFIAVLQIVIADFGRTDFCLLPTNVELELIVCSEKK
                     IRTEEIPDNKRIAFKIFIPKVEGYNYKPMQETQSEILAVASTILFWCSALPDSEIKKK
                     LASSFKNELSSKVFLVRPYWELFLELTSGTDFKARRLAQIGTFDRKISPQRESKEICW
                     IDTPGYGYSNKKAHEFIQNRYRRAITPIRKTLVRLRSSSRFNQWVSDLRCNGYLDWQI
                     LLILSSHVVNFRVNLINSTFHPEHHKVLFMEIVNREESDTDPYFHEELLYDSKNDFAV
                     FTSLIATTSTWGLQIRRDTPDIEAIKRLMDVRYFQSTDDIPHEDIFAPLN"
BASE COUNT          508 a          297 c          318 g          460 t
ORIGIN      
        1 tacgtggcat ttgttaggtt tttaaacagc ttctaagcat gcactatcac catgaacaaa
       61 aacctgacga ttggactgaa gacaaaattc aaggaatggt attccatttt ttgatagtta
      121 aatcattagc taaagctatg ggtggttcag atgctgtagc aaaagtagaa agcccgttac
      181 gtacactgaa tatgccagat gaccttagag aggaaatact taaccccccg ttgcctctgg
      241 aaaaatatga gaatatggca atagaagaaa tactatccaa gacatcagag gaaatttggg
      301 gggctccatt cactgactgc gggccgactc gaatctatcg ctggaaagcg ttggggataa
      361 gttggtttgc ctccaccgag aataggttat caggcctccc ctatgcggaa gaattcattg
      421 ccgttcttca aattgtaatt gctgactttg gacgcacaga cttttgttta ttaccaacaa
      481 acgttgagtt ggaactgatt gtttgtagtg agaaaaaaat tagaactgaa gaaattccag
      541 ataacaagag gatcgcattt aaaattttta ttccaaaagt tgagggctac aattacaaac
      601 caatgcaaga aacgcaatca gaaatcctag ccgtcgctag cactatactt ttttggtgta
      661 gcgctttacc tgactcggag attaagaaaa aattagcgtc ctcctttaaa aatgaacttt
      721 catccaaagt atttttggtc cggccatatt gggagctatt cttagagtta acttctggaa
      781 cagatttcaa ggctcgacgt ttagcccaga ttggtacttt tgatcgaaaa atatcgcctc
      841 aacgagaatc aaaagaaata tgttggatag atactcctgg ctatgggtat tccaataaaa
      901 aagctcacga atttatccag aaccgctatc gaagggcaat aaccccaata agaaaaacac
      961 tcgttcgcct tagatcaagt tctcgcttca atcaatgggt aagtgatttg aggtgtaacg
     1021 gttaccttga ttggcaaatt ttattaatac tttctagtca cgtagtgaat ttccgagtta
     1081 atttaattaa ctccactttt catccggaac accacaaagt tttatttatg gaaatagtga
     1141 atcgtgagga gtcagacaca gacccctatt ttcatgaaga gcttttatat gattctaaaa
     1201 atgattttgc cgtatttact agcctaatag ccactacttc aacatgggga cttcaaatac
     1261 gtagggatac accagatatt gaagcaataa aaagattaat ggatgtacga tattttcagt
     1321 caactgatga tattccacat gaagatatat ttgctccctt gaattaatat gcactgattc
     1381 agacttcgtc tgagtgattt cggagtgcag caattcgagc taaatttgat gtcgtacgaa
     1441 tgaccgctaa tggcacatag tcgcaattcg aaaagctgta ctctggacgt caataacgat
     1501 tgcgccgtat gttatggtca attcgcgccc acagcataaa tcaattaatt taatggcagt
     1561 gacatgttac gtaggaatgt agt
//
LOCUS       DATBTE010000062         6559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_416485, whole genome shotgun sequence.
ACCESSION   DATBTE010000062 DATBTE010000000
VERSION     DATBTE010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416485"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VIG33_02495"
     CDS             complement(<1..235)
                     /locus_tag="VIG33_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8269730.1"
                     /translation="MPRVKLNDTEIYYEDHGPQQAPAIVFSPFLYMDTTMYDPMVAAF
                     SEEYRVIIYDHRGQGQSWHASKKTDLLATTKDAI"
     gene            402..1373
                     /locus_tag="VIG33_02500"
     CDS             402..1373
                     /locus_tag="VIG33_02500"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEY8269731.1"
                     /translation="MNSKSSFYNLDPEKVLQAAENEGFLTTGEFSQLNSYENRVFDIH
                     LEKSLNNHTNNIIAKFYRPHRWSKEAILEEHRFLQDLQAESIPAIAPFTLNSGSTLSE
                     QYGMYVTFFPKVRGRMPQEFVGEELAQIGRLMAQVHNVGSQRKAIHRPVLDTSYYGGW
                     ETLNNLQNWVAPEVLGRYNDAAEMILNQLDDLLNPEEFIRIHGDCHKGNLLHNGRQFF
                     LVDFDDFCTGPVIQDFWMLLSDEGEEGLREREEIIRGYEELREFPDHQWELVPWLRGL
                     RIISYAGWIAKRWEDPSFPRLFPEFNTFRYWAEEVEALEKIAWTNPH"
     gene            complement(1375..1737)
                     /locus_tag="VIG33_02505"
     CDS             complement(1375..1737)
                     /locus_tag="VIG33_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269732.1"
                     /translation="MKFLAFYFSFVSTLAFAQTMGIKDISTDGDTTIKIQKGKEEDKY
                     EITTGTDAIEGDAAPLLKEARANWKTACSEWKKELKELNKENQVISLSCGTMKCSTTA
                     METTCASEGKNKVRVKVK"
     gene            1853..1950
                     /gene="ffs"
                     /locus_tag="VIG33_02510"
     ncRNA           1853..1950
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VIG33_02510"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2003..3610
                     /gene="dnaX"
                     /locus_tag="VIG33_02515"
     CDS             2003..3610
                     /gene="dnaX"
                     /locus_tag="VIG33_02515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY8269733.1"
                     /translation="MSYQVIARKWRPQSFNEVVGQNHITQTLTNALKNNRLHHAILLT
                     GPRGTGKTSSARILAKAIRCPFAVNFVPCDKCDSCMEIAQSRSSDVIELDGASNNGVD
                     AIRDLRDSVGYMPSSGKYKVYIIDEVHMLSTSAFNALLKTLEEPPAHVVFIMATTEAQ
                     KIPQTILSRCQRYDFRRIPIKQITEQLKKICEGENVVAEEEALWMIARQGDGSMRDSL
                     SLLDQVITFANGPLNRQNVVEILGLTSRSLLLETLGALVARNTKAVMAVLEKIATSGF
                     EPHLFSQDVLEMIRNLLLVKVSGDEIAHILELPDSELQALTNLATQVSEEDLHMLFDM
                     ALKGGNDIPKAQDPRIVLEVTLLRMAAAPKITDLESLLRGGARLGGSKASVQKAPPQP
                     RYVTGITLNDKWMAFVDKTRQDDALFAAKIENLLFVNEEAKILRLGVPPKLAFLKEQM
                     NAPGIRTKLQGFIDSYWGSGYSFQVLSSREPVAGESAHALMQKKTQAAEDEVRKQIDE
                     DPMVKTAQLVFKGRIKSVTEIANKGRR"
     gene            3614..3937
                     /locus_tag="VIG33_02520"
     CDS             3614..3937
                     /locus_tag="VIG33_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY8269734.1"
                     /translation="MKGFSGGMAQLMKQANQMQTRMKKVQEELAQQHYEGTSGGGAVT
                     VKVSGEYKMMSLTINPEVVKSGDVEMLQDLILTATNEAVKIARETSQKEMEKVTGGMN
                     VPGLF"
     gene            3942..4556
                     /gene="recR"
                     /locus_tag="VIG33_02525"
     CDS             3942..4556
                     /gene="recR"
                     /locus_tag="VIG33_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY8269735.1"
                     /translation="MLNIPVLEKLVHELSRLPGIGPKTAQRLAYHILKSKDNYITSLS
                     ETLLRVRAEVHTCTTCYNYTDTEECKYCKDVHRSDESICVVEEPSDLMKVEASGAFRG
                     RYHVLHGVISPLEGVNAQDLKIKELIARIDAGLNKQAPLIKEIILALDADLEGDTTTL
                     YLAKLLQGKGVRLSRLAHGVPIGSDIDFIDDRTMGQALENRVEL"
     gene            4556..5149
                     /locus_tag="VIG33_02530"
     CDS             4556..5149
                     /locus_tag="VIG33_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HEY8269736.1"
                     /translation="MSFINYNAKEIHCKVVYYGPSLGGKTTNVQWVYQKTANDQKSKL
                     VALNSDVERTLFFDFLPLNVGDIRGFKTRFHLYTVPGQVVYDASRKLILKGLDGVIFV
                     ADSQIERMDENLEALKNLKRNLEQQGYDIREIPLVMQYNKRDLPNAASLAEMRSALNF
                     YNAPEVEASASEGKGVSESLKIVSKSILNVLKGGSNL"
     gene            5162..6127
                     /gene="nadA"
                     /locus_tag="VIG33_02535"
     CDS             5162..6127
                     /gene="nadA"
                     /locus_tag="VIG33_02535"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEY8269737.1"
                     /translation="MTYDIIADIQRLKKEKNAVILAHYYEEGDIQDIADYVGDSFYLA
                     KMGQKVEQQVILLAGVVFMAESVKILNPTKTVLVPDLEASCSLVKGAPFDKYLAWRQK
                     FPEGVAVTYINSSAEVKTISDVIITSSNAEQIIRSIPEDRKILFGPDQHLGRFLSKKL
                     NRPFELWPGACEVHVLFNARKLYELMLEHPEAVVIAHPECDETVLNYAQVIGSTSRLL
                     EEVEKNPAKKFIVATETGIFHQMQKKRPDATLIQAPVLESGCLCNNCPYMKMNTLEKI
                     KRALETLSPKVEMNETLRLKAQVSLNRMMDITSGKSVAWPSQFHI"
     gene            6333..>6559
                     /locus_tag="VIG33_02540"
     CDS             6333..>6559
                     /locus_tag="VIG33_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269738.1"
                     /translation="MSRPKTKAKTIPNRRVAPRKELTPINVSYITSLSDFTKIAKNCE
                     IVEASATGLLLLIKRDDLLPATLRKTLSLDI"
BASE COUNT         1807 a         1450 c         1479 g         1823 t
ORIGIN      
        1 caatggcatc ttttgtcgtg gccaatagat ctgttttctt cgaggcatgc caactctgac
       61 cttggccccg gtgatcgtag ataatcactc gatactcctc cgagaaggcc gctaccatgg
      121 gatcatacat ggttgtatcc atgtaaagaa aaggactaaa tacaatagcc ggggcttgtt
      181 gcggaccgtg atcttcataa taaatttctg tatcatttag ctttactcta ggcataaatt
      241 tctcctttga agaaacctaa ccacggtcac cgcaaaaaaa aaattaaaac tccggatatt
      301 ataaagacgt caatactgtg gcgtgagaaa ttcacaagat tccaaaaaag tttattttgc
      361 cttatttgcc atgagttctt ctaggaagta tactttttat tatgaattca aaaagctcct
      421 tttacaatct tgatcctgaa aaggttctac aagccgcaga aaacgaaggc ttcctgacca
      481 ccggtgaatt ttcgcagctt aattcctatg aaaatcgtgt attcgatatt cacctggaaa
      541 agtcattaaa taatcataca aacaacatca tagcgaaatt ttatcgaccc catcgctgga
      601 gcaaagaggc tattcttgaa gaacatcgat ttcttcaaga tctgcaagca gagtccattc
      661 ccgcaattgc gcctttcact ttaaattctg gaagcactct cagcgaacaa tatggtatgt
      721 atgtcacttt ctttccgaaa gtccggggta ggatgcccca agaatttgtt ggtgaagagc
      781 ttgcccaaat cggccgtttg atggcccagg ttcataatgt cggaagtcag cgcaaagcga
      841 ttcatcgacc cgtccttgat acaagctatt acggtggctg ggagactctt aacaatttgc
      901 aaaactgggt ggctcctgaa gtgctcggtc ggtataatga cgccgctgag atgattctga
      961 atcagcttga tgatctttta aatccagagg agtttattcg aattcatggc gactgccata
     1021 aaggcaattt gcttcataat ggtcgccagt tcttcttagt cgacttcgat gatttttgca
     1081 cgggccccgt tattcaggac ttctggatgc tactcagtga tgagggcgaa gaaggcctgc
     1141 gcgaacgcga agagattatc agaggctatg aagaactccg tgaatttcca gatcatcaat
     1201 gggaattagt accgtggctg cggggtctgc ggattatttc ttatgcaggc tggattgcaa
     1261 aacgctggga agatccaagc ttcccgcgtt tatttccgga gtttaatacc tttcgatatt
     1321 gggcggaaga ggtggaagcc ctcgaaaaaa tcgcttggac aaacccccac taatttattt
     1381 tacttttact ctgaccttgt ttttgccctc agaagcgcag gtggtttcca tggctgtggt
     1441 tgaacatttc atagttccac agctcaagga gatcacttga ttttctttgt taagttcttt
     1501 gagttccttt ttccattcgc tgcaagccgt tttccaattg gcacgagctt ctttcaaaag
     1561 aggagcggca tcgccttcga tcgcatccgt gccggtcgtg atttcgtatt tatcctcttc
     1621 tttccctttt tggatcttaa tggttgtatc accatcggtg gaaatatctt tgatccccat
     1681 ggtttgcgcg aaagccaatg tgctgacaaa gctaaaataa aatgctagaa atttcatggt
     1741 ttctccttgg tttagtgaat ttaggcaaaa ctatcaggtt tgttcaggac tttttcgcat
     1801 gcgctaagcg ttatcgcgtg aaatacaggc aggcgagagt tactacagcc ttggccgggt
     1861 cccatacaat agagccctta ctgaatccgc taggtccgga aggaagcaac ggcgtttaag
     1921 ggactatgtg atatggggtg ctcggccact tgtctttctg gatcttttgg gatctttttg
     1981 gcagactttt agagaggtct ctttgtctta tcaggtcatt gcacgtaaat ggcgtccaca
     2041 gtctttcaac gaagttgttg gacagaacca tattactcaa acactgacca atgcacttaa
     2101 aaacaatcgt cttcatcatg ccattctgct tacaggtcct cggggaactg gaaaaacttc
     2161 ttcagcacgt atcttggcca aggccattcg ttgtcccttt gctgtgaact ttgtcccttg
     2221 tgataaatgt gattcttgta tggaaattgc gcaaagtcgc agctctgatg tcatcgaact
     2281 tgatggagcc agtaataacg gcgtcgatgc tatccgtgat ttacgagact ctgtcggcta
     2341 tatgccctct tccggcaaat acaaagttta catcattgat gaagtgcaca tgctttccac
     2401 aagtgctttc aacgctcttt taaaaactct cgaggagccg cccgcccatg tggttttcat
     2461 catggcgacc acggaagcgc aaaaaatccc gcaaacaatt ttgtctcggt gccaacgcta
     2521 tgacttccgt cgcattccga taaagcaaat caccgagcaa cttaaaaaaa tctgtgaggg
     2581 tgaaaacgtc gttgctgaag aagaagctct gtggatgatt gctcgtcaag gtgatggatc
     2641 catgcgcgac agcttaagcc tgctcgatca ggtcatcacc tttgccaatg gtccgctcaa
     2701 ccgccaaaat gtcgttgaga ttttaggtct caccagtcgc agcttgcttt tggaaacact
     2761 cggcgctctg gtggcccgca ataccaaggc tgtgatggct gttttagaaa aaatagcgac
     2821 ttcaggtttt gaaccgcatt tattttcgca agacgtcctc gaaatgatcc gcaatctgct
     2881 cttggtgaaa gtttcaggag acgagatcgc ccatattctc gagcttccgg attctgaact
     2941 tcaagctctg acgaacctcg caacgcaggt ttctgaagaa gatttgcata tgctttttga
     3001 tatggccctc aagggcggca atgatatccc taaagctcaa gatccacgca ttgttttaga
     3061 agtcaccctg ctccgtatgg cagccgcacc aaagatcacg gatctagaaa gcttacttag
     3121 aggtggggcc cggctaggcg gctctaaggc atccgttcag aaggcccctc cacagcctcg
     3181 ctatgttacc gggatcaccc ttaatgacaa gtggatggca ttcgtggata aaactcgtca
     3241 agatgatgcc ctttttgccg ctaagattga aaatcttctt ttcgtgaacg aagaggccaa
     3301 aatcttaagg ttgggtgtcc cccccaaatt ggctttcctt aaagagcaaa tgaatgcccc
     3361 tggtatccgc accaagttac aaggatttat tgattcatat tggggctctg gttattcttt
     3421 ccaagttctt agtagccggg aacccgtggc gggcgaaagc gctcatgccc tcatgcagaa
     3481 aaaaactcag gcggcagagg acgaagttcg taaacaaatt gatgaagatc caatggtaaa
     3541 aacagcccag ttggttttta aagggcgaat caaatcagtg acagagattg ccaataaagg
     3601 caggaggtag cacatgaaag gtttttccgg tggcatggct caattaatga agcaagctaa
     3661 ccaaatgcaa actcgtatga agaaggttca agaagaattg gctcagcagc actacgaagg
     3721 aacttctggc ggtggcgcgg tcactgtcaa agtgagcggc gaatacaaaa tgatgtccct
     3781 cacgatcaat cctgaagtag tcaaatcagg agatgtggaa atgctgcaag atttaattct
     3841 gactgccaca aatgaagcag taaaaatcgc tcgtgaaacc tctcaaaaag agatggaaaa
     3901 agtaactggc ggcatgaacg ttcctggtct attctagtag aatgctgaat attccagtcc
     3961 ttgaaaaact tgttcatgaa ctcagccgtc ttccaggcat tggccctaag acggctcagc
     4021 gtctggccta tcatattttg aagtccaaag ataattacat caccagcctg agtgagacct
     4081 tgcttcgagt gcgcgcggaa gttcatactt gtacaacttg ttataactac accgataccg
     4141 aggaatgcaa atactgcaaa gatgtgcatc gttctgatga gtctatctgc gtggtggagg
     4201 aaccttcaga ccttatgaag gtagaggcct caggagcctt tcggggccga taccatgttt
     4261 tgcatggagt gatctcgcct ttagaagggg ttaatgccca agatcttaaa attaaagaac
     4321 tgattgcgcg tattgacgcg gggcttaaca aacaagctcc attaattaaa gagatcattc
     4381 tcgctctgga tgcggatctt gagggtgata ccactacttt gtatcttgca aagcttcttc
     4441 aaggcaaagg tgtgcgatta agtcgcttgg ctcacggagt tcctattggc agcgacattg
     4501 atttcataga tgatagaact atgggtcaag ccttagaaaa ccgtgtggag ctgtaatgtc
     4561 ttttattaat tacaacgcca aggaaattca ctgcaaagtt gtttactacg gtccatcatt
     4621 gggtggtaag acaaccaacg ttcagtgggt ctatcaaaaa acagcgaacg atcaaaaatc
     4681 gaaactggtg gcattaaatt ctgacgtcga aagaactctc ttttttgact ttttacccct
     4741 taatgtgggc gatatccgtg gttttaaaac tcgtttccat ttgtacacgg tccctggcca
     4801 ggttgtttat gatgcctctc gcaagttgat tctgaaaggc ttggatgggg tcattttcgt
     4861 tgcagattca cagattgaac gcatggatga gaatctcgaa gcacttaaga acctaaaaag
     4921 aaacctcgaa cagcagggtt atgatatccg cgagattcct ttagttatgc agtataacaa
     4981 acgagatctt ccaaatgcgg cttctttggc agagatgaga agcgctttga acttctataa
     5041 tgctcctgaa gtagaggcct cggcgtctga aggaaaaggg gtctccgaat cccttaaaat
     5101 cgtttctaag tccatcctga atgtgttaaa aggcggctct aacctctagt tggaggcctc
     5161 gatgacctac gatattattg ccgatattca gcgcctcaaa aaagagaaaa atgcggttat
     5221 tctcgcccac tattatgaag agggtgatat ccaagatatc gctgactatg tcggggatag
     5281 cttctatttg gctaagatgg ggcaaaaagt agagcagcaa gtgatcttac tggcaggcgt
     5341 cgtttttatg gcggaaagtg tgaaaatcct gaatcccacc aaaacggtcc tggtaccgga
     5401 cctcgaagcc agttgctcct tagtaaaagg cgcccccttc gataaatact tggcttggcg
     5461 tcaaaaattc cccgaaggcg tcgctgtcac ttatattaat tccagcgctg aagtaaaaac
     5521 catttctgac gttatcatca cttctagcaa tgccgagcag atcattcgct ctattcctga
     5581 ggaccgtaaa attctttttg gacctgacca gcacttggga cgttttctat ccaagaagct
     5641 caaccgtcca tttgagctat ggcccggagc ttgtgaagtg catgtgcttt ttaatgcccg
     5701 taagctctat gagctgatgt tggaacatcc agaagccgtg gtgatcgctc atcctgagtg
     5761 tgatgaaacg gttttaaatt atgcgcaagt tataggttca acctcccgct tacttgaaga
     5821 agtcgagaaa aacccggcga agaagtttat tgttgcaaca gagactggca tctttcacca
     5881 aatgcagaaa aaacgtccgg acgcgacttt gatccaggcc ccggttttag agagtggctg
     5941 cctttgcaat aattgccctt atatgaaaat gaacaccctg gaaaagatca agcgagctct
     6001 tgagactctc tcaccaaaag tagagatgaa tgaaacactg cgattaaaag ctcaagtgtc
     6061 tttaaatcgc atgatggata tcaccagcgg aaagtccgtt gcttggccct ctcaatttca
     6121 tatttaatta gcagaatgta ttattccata tctattcgtt gatgtctcat tttgagaata
     6181 acttgggccc attcaaaagc tttgtagata ttttattcgc tctggtatta aatccacctt
     6241 tttaggtttg atattaaact ttggtcgagt ttaaccgatg agtctagtga gaggtcaatg
     6301 gaccaacgaa ctgatcacca gaaaggtgtg caatgagtcg ccctaaaacc aaagccaaga
     6361 ctatacctaa cagaagggtt gcgccacgca aggaactcac ccccattaat gtgtcttata
     6421 tcacatcact ttcagacttc acaaaaattg caaaaaattg tgagattgtt gaagcctcag
     6481 cgactggact gttgctactt ataaaacgtg atgatctttt gcctgccacc ctaagaaaaa
     6541 ccctcagctt agatatttt
//
LOCUS       DATBTE010000063         4988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_826623, whole genome shotgun sequence.
ACCESSION   DATBTE010000063 DATBTE010000000
VERSION     DATBTE010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826623"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VIG33_02545"
     CDS             complement(<1..527)
                     /locus_tag="VIG33_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269739.1"
                     /translation="MKRSREFFLWAFFLAVCFWAGYLRGYLPLSFKQGLFVKGPVKIL
                     NATPLDFPVDFIKALEEDFGQKVEVQRVKGWDEVQAKMVTKSGVHLLLAPTFWAQDLS
                     RQSLILKLNSLQTKIEKRISPDFISLQDKNLSALPLYWTVTDFRVHKDSNLGETLERA
                     LENKALSEIHLYPDT"
     gene            complement(514..1512)
                     /locus_tag="VIG33_02550"
     CDS             complement(514..1512)
                     /locus_tag="VIG33_02550"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEY8269740.1"
                     /translation="MLLDPGFNLTLRPMKHPVLYEMYKNGIKNTWTVDEIDFSTDLVD
                     LHSKMTPAERHLISRLVAFFATGDSIVGNNLVLNFYKHVNSPEGRMYLSRQLYEEALH
                     VQFYLTLLDTYIPNQDERAAAFAAIETIPSIKKKADFCFKWIDSIHDFDTLKTKEDRR
                     KFLMNMICFATCVEGLFFFAAFAYVYFLRSKGLLTGLASGTNWVFRDESMHMAFALEV
                     IKIARQEEPDLFNEQMEDMVVEMIEDAINCEMEFAQDVLQFGLAGMSAKDMRQYLEFV
                     ADQRLESLNIAPRYKAKNPFSFMELQDMQELANFFERRVSAYQMGVSGSVSFDETF"
     gene            complement(1542..3983)
                     /locus_tag="VIG33_02555"
     CDS             complement(1542..3983)
                     /locus_tag="VIG33_02555"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEY8269741.1"
                     /translation="MLNQEQGFQSEAHSNSYSDTTPHIMRVKKRDGTLEPVDVTKIVE
                     RVTRCCQGLTQIDPLRVATKAISGLYDGATTNELDNLCIQTASLLIGEEPQYSRLAAR
                     LLATYVDEEVRSQKIGSFADAINLGYSMGLMSEALYNFVKNNKAALCAAIEPYRTDRF
                     EYFGLRTVYDRYLLKNPTSRQVIETPQYFFMRVACGLAQSVDEAIEFYRLISSHDYMA
                     STPTLFNSGTLRPQMSSCYLLDSPEDDLRAIYDKYTDIALLSKFAGGIGVAFHRIRSR
                     GSLIKGTNGHSNGIIPWLKTMDSSVAAVNQGGKRKGAACVYLETWHADIEEFLELRDN
                     TGDEAKRTHNLNLANWVPDLFMKRVEEDAMWSLFDPRVVPYFTDIYGPEFEKAYIEAE
                     EKKMYVKQIKARDLYVRMMKTLAQTGNGWMTWKDACNLKANQVGRPQNVIHLSNLCTE
                     ILEVTSNKETAVCNLGSVNLARHVINGQFDFEKLASTVRTAIKYLDRVVDINFYPIET
                     AKDSNHKWRPVGLGIMGLQDAFFQLKLPFDSTEARELSARIQEDIYYNAIYASCDLAE
                     KHGPHGAYEETRAAQGQLQYDLWGVTPIQPERFLRLKERIKKHGLRNSLVIAIAPTAT
                     IASIVGCYEATEPQISNLFKRETLSGEFMQINRYLVSDLKALNLWNEDIRNEIKMADG
                     SIQDVAAIPPHLKELYRNVWEVPMKALIDMGAERGAYIDQSQSLNLFMESPTIGKLSS
                     MYMYAWKKGLKTTYYLRSRPATSIAKTTVKKTNTGTATLTETTIVETAPKKEYTDAEV
                     IACSLENPEACEACQ"
     gene            complement(4559..>4988)
                     /locus_tag="VIG33_02560"
     CDS             complement(4559..>4988)
                     /locus_tag="VIG33_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEY8269742.1"
                     /translation="GNPGETDEHIIQTAEIVNKLPLTNIKLHNLHALKNTPLEEMYHR
                     GEFLPIDRETYSHRVKIFLQHLAPHLAMHRLAAFASRWEELVAPSWTSDKMGTHQYII
                     DFLRHEKAYQSQMFKADDVETQTFIERIREKSVPAKLTRL"
BASE COUNT         1378 a         1077 c         1122 g         1411 t
ORIGIN      
        1 tcagtgtccg gataaagatg aatttctgag agagctttat tctcaagagc ccgctcgaga
       61 gtttctccaa ggttgctgtc tttatgtact cgaaaatccg tgacagtcca atataaggga
      121 agagcactca gatttttatc ttgaagggaa atgaaatccg gagagattct tttttctatt
      181 tttgtctgca aagagttgag tttaagaata agggattgac gggagagatc ctgagcccaa
      241 aaggtagggg ctaagagtaa gtgaactccg cttttagtga ccatttttgc ttgaacttca
      301 tcccaaccct taactctttg aacttctact ttttgtccaa aatcttcttc aagagctttg
      361 atgaaatcga cgggaaaatc caaaggagtt gcatttaaga tttttacggg acctttaacg
      421 aagagacctt gtttaaagga taagggcaga taccctctga ggtaacctgc ccaaaagcac
      481 acagccaaaa aaaaggccca aagaaaaaat tccctagaac gtttcatcaa acgatacgct
      541 tccggaaact cccatttggt aggcggaaac tcttctctcg aagaagtttg caagctcttg
      601 catgtcttga agctccatga aagagaatgg gtttttcgcc ttataacgag gcgctatatt
      661 caaactttcc aagcgctggt cggcgacgaa ctctaaatac tgtctcatgt ctttagcgga
      721 catccctgcc aagccaaatt gaagaacatc ctgggcgaat tccatctcgc aattgatggc
      781 atcttcaatc atttccacaa ccatgtcttc catttgctca ttgaaaagat cgggttcttc
      841 ttggcgagct attttaatca cttccaaggc aaaagccatg tgcatgcttt cgtcacggaa
      901 aacccagttg gtgccactag ctaatccagt aagtaatcct tttgaacgca agaaataaac
      961 atacgcaaag gccgcgaaga aaaataaacc ttccacgcag gttgcaaagc aaatcatatt
     1021 catcaaaaat ttacgacggt cctctttggt ttttaaagtg tcaaagtcgt gaatagaatc
     1081 aatccactta aagcagaagt ccgctttctt tttgatggaa ggaatggttt caatggcggc
     1141 gaaagccgcg gctctctcgt cttgattggg gatataagta tccaaaagcg tcagatagaa
     1201 ttggacatgc aaagcctctt cataaagctg acgggacaaa tacattcggc cttctggaga
     1261 gttcacatgc ttatagaaat tcagcacgag attgttgcca acgatggagt ctccggtcgc
     1321 aaagaaagcc actaaacgtg aaattaaatg tctttcagca ggggtcattt tggagtgtag
     1381 atccaccaag tccgtcgaga aatcaatttc gtccacggtc caagtattct taatcccatt
     1441 cttgtacatt tcatagagta cgggatgttt cattgggcgg agggtcaggt taaaaccagg
     1501 atcaagtaac atgggtcatt ccttcttaag tagtcattgg attattggca agcttcgcag
     1561 gcttctggat tttctagaga gcaagcgatc acttcagcat ctgtatattc ttttttagga
     1621 gccgtctcga caatggttgt ttcggtgaga gtcgcagtcc ctgtgttggt ttttttaaca
     1681 gtggtttttg caatgctcgt cgcaggtctt gaacggaggt aataagtcgt tttcagacct
     1741 tttttccacg catacatgta catgctggac agtttgccga tggtagggct ttccatgaaa
     1801 agattcaaag attgagattg atcaatgtag gcaccccgtt cagcgcccat atcaatcaat
     1861 gccttcattg gaacttccca gacatttctg taaagttctt tcaagtgggg cggaatggcg
     1921 gcgacgtctt gaatagaacc atcggccatt ttgatttcat tgcgaatgtc ttcgttccaa
     1981 agattgagag ctttcaaatc agacaccaag tagcggttga tttgcataaa ctcgcctgaa
     2041 agggtttctc gcttgaagag attgctgatt tgaggttcgg tcgcttcata gcagcccaca
     2101 atggaagcga tggttgccgt tggcgcgatg gcgatcacca aggagtttct caagccatgc
     2161 tttttaatac gttctttcaa gcgaaggaag cgttcaggtt ggattggggt tacaccccaa
     2221 agatcatact gaagttgacc ttgtgccgcg cgggtttctt cgtaagcgcc atgggggcca
     2281 tgtttttcag ccaagtcaca ggatgcatag atcgcattgt aatagatgtc ctcttggatt
     2341 cgcgcagaca actcacgggc ttcagtggaa tcaaagggca atttcaattg aaagaaggca
     2401 tcttgtaatc ccataatacc aagacctact ggacgccact tgtgattgct gtctttggcc
     2461 gtctcaatgg ggtagaaatt aatatcgaca acgcgatcaa gatatttgat agcggtgcgc
     2521 acagtgctcg caagcttttc aaaatcgaac tgaccattaa tcacgtggcg agcgagattc
     2581 actgaaccta aattgcacac cgccgtttct ttattggaag tcacttctaa gatctcagtg
     2641 cagagatttg aaagatggat cacattctga ggtcgcccca cttgattggc cttcaggttg
     2701 caagcatctt tccaagtcat ccagccattg ccggtttgag ctaaagtctt catcatgcgt
     2761 acatacaaat cccgagcttt gatttgcttt acgtacattt tcttttcttc ggcttcaata
     2821 taggcttttt cgaattcagg gccataaatg tctgtgaaat aaggaaccac acgtgggtca
     2881 aataaagacc acatcgcatc ctcttccaca cgtttcataa ataagtccgg aacccaattt
     2941 gccaagttca ggttgtgggt gcgtttggct tcatcaccgg tgttatcacg cagctcaaga
     3001 aattcttcaa tgtccgcatg ccaagtttcc aaatagacac aggcggctcc cttgcgttta
     3061 ccaccttgat ttacagcggc taccgaagag tccatggttt ttaaccaagg aatgattcca
     3121 ttggagtgac cattggttcc tttaatgagg gagccccggg agcgaatgcg atggaaagcc
     3181 acgccaattc cgccggcaaa tttagagagt aaagctatgt ccgtgtattt atcgtaaatg
     3241 gcacgcaaat catcctcagg ggagtcgagt aaatagcaag aagacatctg tggacgaaga
     3301 gttccagaat taaacaaagt gggcgttgag gccatataat catgggagga aatcaaccga
     3361 taaaactcaa tggcctcatc aacactttga gccaggccgc aagccacgcg cataaagaag
     3421 tactggggag tctcaatgac ctggcgactt gtggggttct tcaagagata acgatcatag
     3481 accgtgcgca ggccgaagta ttcgaagcga tccgtgcggt aaggttcaat agctgcacat
     3541 aaagcggcct tgttattttt gacgaagtta taaagagctt cggacatcaa acccatggaa
     3601 taacccaagt tgatcgcatc ggcaaaagaa ccaattttct gtgagcgaac ttcttcatcc
     3661 acataggttg ccaggaggcg ggcggctaga cgagaatact gaggttcctc accaatcagt
     3721 aaacttgctg tctgaataca aagattatcc aactcatttg tcgtagcgcc atcataaaga
     3781 ccactgatgg ccttggtggc aacacgcagg ggatctattt gagtgagtcc ttggcagcaa
     3841 cgagtcacgc gttctactat tttcgtaaca tctactggct ctagtgtgcc gtcgcgtttc
     3901 ttaactctca taatatgcgg tgttgtatcc gaataagaat tagaatgagc ctcagattga
     3961 aagccctgct cctgattcag catagtggtt ccctcatggc ctgtttcgca ggcgaaaaaa
     4021 agtcacccag tttctacccc tccgtaaaaa ccgaacgata ttgtctgttg ttaatcgtgt
     4081 ttgctagtac tgattaatca gttcacttca ttcctgtgaa ttgaacataa ttaatgtggt
     4141 cgagaaagag agcttcttcc aatcggatcc tcctgttgac aagaacgtta acagttagaa
     4201 ttgctctttt aactcctgga ctttgatctc actagggctt ttggctttta gtgtgaccac
     4261 tagatctagg ggttcgtcag agtggccata tccatatggc ctatcttcct cacgcttcaa
     4321 ggatcttcac tttttgaaat ggagtcaaac ctaaataggg aaaaaattac aaaaacatct
     4381 ggcgctgctt atcatttaag catggatttt gatcttcttg acaagtctgt cttacaaaaa
     4441 aaagctgctt ttttttgcat tgtacatagc gaaaagtcat ctaaagactt gaaagtacga
     4501 aataacaacg gattaacgca gagtctgtga tcatctatct gtgaggatct taaattattc
     4561 agagtcttgt taatttggcg ggaacgcttt tttcacggat gcgctcgatg aaggtctggg
     4621 tttcaacatc atctgctttg aacatctggc tttgatacgc tttttcatgc cgtaagaaat
     4681 caatgatgta ttgatgagtg cccattttat ctgaggtcca cgaaggagct accaactctt
     4741 cccagcgaga cgcaaaagcc gcgagcctgt gcattgccaa gtgcggtgct aagtgctgca
     4801 aaaagatctt tactcgatga gaataggttt ctcggtcaat agggaggaac tctccgcgat
     4861 gatacatctc ctcaagagga gtgtttttaa gggcgtgcaa gttgtgcagt tttatattgg
     4921 tgagaggcaa tttattcaca atttctgcag tttgaataat atgttcatcc gtttctccgg
     4981 gattgcca
//
LOCUS       DATBTE010000064         6101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1441721, whole genome shotgun sequence.
ACCESSION   DATBTE010000064 DATBTE010000000
VERSION     DATBTE010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441721"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..2102)
                     /locus_tag="VIG33_02565"
     CDS             complement(168..2102)
                     /locus_tag="VIG33_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8269743.1"
                     /translation="MEYSKVKILILEDDVTLGEALKEAFTRAGHEVFLVVEPEKATEI
                     IYKEKIDFLFIDCLLPQMMGIDYITKIRNELQSKSKFKIVLMSGIYTDSDFVQDATKK
                     TQAVAFLKKPFDLNEALKLVKGTEAHSKEEPSPRKNLYQIFARNMVSNREKRKLIEAL
                     EEVSGFDLPFIYSLLVEMKSSGHLNIYGVDGNVSGVSFSNGVIVGVDIEDKTTYLGEM
                     LIQSGYALPEDVQEALSDKSPRRLGQRLMQANKLSPHAFDLILTEQMNIRLSRTIIDQ
                     MIRINFSTAEVEMIEPSIDSDSLSYFLHDWIASKLSSVWLKKMYLVWENYRIARSSAY
                     RQDHPALKMSLVQSLDGFIDKIDQGTTLAELLAIPGYHEAAVYKAVHFLLTKGLIIFL
                     KSQVLVSPQEKLKALRKIFADIQSKNNFQVVEYFGLGQESVATVSGMMEEFLPLLGEK
                     PKDPKEEVYKLWTQIHTRVTEACTSFLDVGQRQQYKQNNLKSEAEKILKANSLIEDAK
                     QALQLNQYTKAAALMAQAVELNPESGQVHLYHAWAKIGTLDPQKRMQQLKEIEFELMQ
                     VPPDEKYDALFPFVTGLVQKAKGDIVGARKSFEKCLALNSSMIVARRELGQLTSVNAN
                     KEDILNMDLKKMVSGFFKKK"
     gene            complement(2155..2625)
                     /locus_tag="VIG33_02570"
     CDS             complement(2155..2625)
                     /locus_tag="VIG33_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269744.1"
                     /translation="MAKFDVKLKKSEEKVDVILTGSIDEDADFNQANLAGAGNIEIEM
                     SGLKSINSCGIREWIKWLSAVPTAKITFNGCPKVIVDQINMVDGFLPANAKVQSFYVP
                     YYNDESGSEKNVLFSLGKEFTENSVNPPASVKDNEGNVMEMDIIESKYFKFIKK"
     gene            complement(2676..4511)
                     /locus_tag="VIG33_02575"
     CDS             complement(2676..4511)
                     /locus_tag="VIG33_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY8269745.1"
                     /translation="MKNSGISIRYKILLLLTVIPLLTLSIYLFLAVQVFENDKVAYVF
                     DSSSNVSGAMGSQVKAQLNSFLNEVKPVFQDFLTNNEFTPQTHLIFSNDDNLESVVAF
                     KFNAVAKKFEKAGLVEKTIGSTDGILLGLEKVLPLYFTELEQMGRIVKSPYHDDRLFI
                     FEKVKEKEGFSSVFLLIVHLSEVAEVFRAQSSQRLYLISQDGTILFGPEGTSGAFMQS
                     SFPLRFLERKDNKVAQGAETIKDAENTDWLVSYSRAGFGDLMVVSTVEKKKALSAVGI
                     LIRKSLIFFGILLAVTAVISLVASSSLTSALTSLFQATKKVSEGDFNIRVKVTSSDEV
                     GALAENFNLMAEEVSRLLQETAQKARMESELQTAKTVQETLFPETQAKIGPLSIAGFY
                     EPASECGGDWWHYCQVGRKIFLWIGDATGHGAPAALITSAAKSAATIIEKLNVTPAQA
                     LELLNRSIYDVSKGRIMMTFFLGAYDPDTSELVYVNASHEAPFLIKKSNDPLKKKDLV
                     PLNEVNNPRLGQDRDTKYKQTTVQLAPEDMVFFYTDGIPDIQNKAKESWGERQFIKAI
                     VSANQDYPTANVSVNRLVQMMQDYRQGGTLIDDVTFFVMKNDLNL"
     gene            4670..>6101
                     /locus_tag="VIG33_02580"
     CDS             4670..>6101
                     /locus_tag="VIG33_02580"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEY8269746.1"
                     /translation="MMILLVLSYFLYDDSLLFPSELQKQTPIGIIGSSNKDVRKKNIK
                     DFVWLPGNQKDQVYEQDSIFTGDDSQASIQLSDGSVIKIEENSLVNLNTKNGQMQLDL
                     RFGQFAGNGKTPLHIKTGNEEYTIQGNDAQFEINRSKTGVMDVKVLSGTAEISSKVGK
                     QKLVANESLKISKHKTEKNEVDSEVEAKIHLLTKNDLLVIRGDKTSTDKLPISFEWKS
                     QGPLVQYQIEIADTEDFIKILTLQSTAERKITLKDSFKEGAYFWRVKGLDIKQQTLAI
                     SEKHKFSLRDLAAPRIMTPDNQSTITKKIIPSDKELQTNTQIAWEADASIESFEWQLS
                     KTAEFTEIINEKSLRGKSLTTPDLGEGTYFTRVRGFDKSKNPSQWSEVRVFNFAFQTE
                     PKPPAPILAQKHIRFAIPKLEGRAPSTATSPQMAWSAVDHIKTYHWEIANNPRFTGAQ
                     AADTPATKAVWTQYKPGKFYFRVFARS"
BASE COUNT         1830 a         1374 c         1156 g         1741 t
ORIGIN      
        1 tgcagttaag caccttagcc tattaccacg tcaataagcg cttcagctgg aactatgggc
       61 taggaataaa tcaatatagc tttgatcccg cccaacttaa ttcccaattt ccaatgcaat
      121 ttgaagtaag gggcggcgca agctatcgct tttaatagtt caaacaatca cttcttttta
      181 aagaagccag agaccatttt tttgaggtcc atattcagaa tatcctcttt attggcattg
      241 acggaagtta actggccgag ttcacggcga gccacaatca tagaagaatt cagggctaaa
      301 cacttttcaa aggatttgcg agcccccacg atgtcccctt tggccttttg tactaaacca
      361 gtgacaaacg gaaagagcgc atcatatttc tcatcggggg ggacctgcat aagctcaaat
      421 tcaatttctt taagttgctg cattctcttt tgcggatcga gagttccgat tttagcccat
      481 gcatggtaca aatgcacttg tccactctca ggatttaatt ccacagcttg agccattaaa
      541 gccgccgctt tggtgtattg gttgagttgc aaagcttgct tggcatcttc gatcaatgaa
      601 ttggctttaa gaatcttttc cgcttcggat tttaagttgt tttgcttgta ttgctgacgc
      661 tgtcctacat ctaaaaagga cgtacaggct tctgttacac gcgtatgaat ttgcgtccac
      721 agcttgtaaa cttcctcttt tggatccttc ggtttttccc caagcagtgg caaaaactcc
      781 tccatcatcc cgctcacggt ggcgacactc tcctgcccta ggccgaagta ttccaccact
      841 tgaaaattat ttttactttg gatatccgca aagatctttc tcaatgcctt caatttttct
      901 tgtgggctca ccaagacttg tgactttaaa aagataatca accccttcgt taaaaggaag
      961 tgcaccgctt tataaactgc ggcttcatga tacccaggaa tcgctaagag ttctgccaag
     1021 gtggttccct gatctatttt atcaataaat ccatcgagac tttgaaccag agacattttc
     1081 aaagccggat gatcctggcg gtaagcggaa cttcgagcaa ttcgataatt ttcccatact
     1141 aaatacattt ttttcaacca gacagaactc aatttcgagg ctatccaatc atgcaaaaaa
     1201 taggacaaag aatctgaatc aatacttggc tcaatcatct caacttctgc ggttgaaaaa
     1261 ttgatgcgaa tcatttgatc tataatcgtt ctggaaagac ggatattcat ttgttccgtc
     1321 aaaattaaat caaacgcatg aggactcagc ttattcgcct gcattaaacg ctgcccgagg
     1381 cgacgtggac ttttgtcact aagggcttct tgcacatctt caggcaaggc ataaccgctc
     1441 tgaataagca tttcaccaag ataagttgtt ttatcttcga tatccacacc cacgatcaca
     1501 ccatttgaaa aggagacgcc ggaaacattg ccatcaaccc catagatatt taaatggcca
     1561 gaacttttca tttccaccag caagctgtaa atgaaaggca aatcaaaacc actgacctct
     1621 tcaagagctt cgataagttt ccttttttcg cggttagaaa ccatgttgcg tgcgaaaatt
     1681 tggtatagat ttttccgagg cgaaggctct tctttagagt gagcctctgt tccctttact
     1741 aatttcagag cttcatttaa gtcgaagggt tttttaagaa aagccacggc ttgggtcttc
     1801 ttggtcgcat cttggacaaa atccgagtcc gtataaatac cgctcatgag cacgatttta
     1861 aattttgatt tagactgcaa ttcattgcga atttttgtga tataatcaat gcccatcatt
     1921 tgcggcaaca aacaatctat aaagagaaaa tcaattttct ctttatagat aatctccgtc
     1981 gccttttctg gttcgaccac caaaaagact tcatgaccag cacgggtgaa ggcttctttg
     2041 agagcttccc ccaaagtgac gtcatcttct aagattaaaa tctttacttt tgaatattcc
     2101 acgtatcttc ctttcggaaa aaaagacgtg aaactcaaca agacttagag ctggctattt
     2161 tttaataaat ttaaaatatt ttgattcaat gatgtccatc tccatgacat taccctcatt
     2221 atcttttact gaggcagggg gattgaccga gttttcagtg aactctttcc caagggaaaa
     2281 aagcacattt ttctctgaac ccgactcatc attgtaataa ggtacataaa aagattgcac
     2341 ttttgcattc gctggtaaaa agccgtccac catattgatc tgatcaacaa tcaccttagg
     2401 acatccatta aaagtgatct ttgcggtcgg aacggcactt aaccatttaa tccactcacg
     2461 aattccacaa gaattaatgc ttttcaaacc actcatttcg atttcaatat taccagcacc
     2521 cgccagattt gcctgattga aatcagcatc ttcatcaata gaacctgtca aaatgacatc
     2581 aactttttct tcggattttt tcaattttac gtcaaatttt gccacaacgt tctccttcaa
     2641 ataacagagt atacacttca ttatcaaata taattttaaa ggttcaaatc atttttcatt
     2701 acaaaaaatg tcacatcatc aatgagagtt ccgccttgac gatagtcctg catcatttgc
     2761 actaaacgat tcacggaaac atttgcggtt ggataatcct gattggcact cacaatggct
     2821 ttaataaact gacgttctcc ccagctctct ttagccttat tttgaatatc gggaatgcca
     2881 tccgtataaa agaaaaccat atcttcagga gcaagttgga ccgtggtttg cttgtacttg
     2941 gtgtctcgat cctgtcctaa acgaggatta ttcacttcgt tcagagggac caaatctttt
     3001 ttcttcaatg gatcattgct tttcttaata agaaagggag cttcgtggga ggcattaacg
     3061 taaaccaact cagaagtatc gggatcataa gctcccaaaa aaaaggtcat cataatacgc
     3121 cctttagaga cgtcatagat agagcgattc aacagctcaa gggcttgggc cggcgtgaca
     3181 ttaagttttt caataattgt tgcagctgat ttcgcggcac ttgtaatcaa ggccgccgga
     3241 gccccatggc cggtcgcatc tccgatccat aaaaaaatct tacgcccgac ttggcaataa
     3301 tgccaccaat cgccgccaca ctcgctggct ggctcataaa aacctgcaat ggataaggga
     3361 ccgatctttg cttgagtttc tggaaagaga gtttcctgga ccgtcttggc tgtctgcaac
     3421 tctgattcca tacgcgcttt ttgggcggtt tcttgcagca gacgcgacac ttcttcggcc
     3481 atcaaattga aattttccgc cagagcgccc acttcatctg aagatgtcac cttcactcga
     3541 atgttgaaat ccccctcaga aacctttttc gtggcctgaa acagcgaggt gagggcactc
     3601 gtcaaagaac tcgaagcgac caagctaata accgcggtca ccgctaaaag gattccaaag
     3661 aagatcaaag atttacggat aagtatgccg acagcgctca aggccttttt cttttccacg
     3721 gtggaaacca ccattaagtc accaaaacct gcacgagaat acgataccag ccaatctgta
     3781 ttttccgcgt ccttgatcgt ctcagcgccc tgggcgactt tgttgtcttt cctctctaaa
     3841 aatcgcagag gaaaactgga ttgcataaag gctccgctgg ttccttcggg cccaaataaa
     3901 atcgttccat cttgtgaaat taaatagagt ctttgagaag attgagcccg aaaaacctcc
     3961 gccacctccg aaaggtgaac gatcaataaa aagacagagg agaatccttc tttttccttc
     4021 actttttcaa atatgaataa acgatcatca tgatacggtg acttgacgat acgccccatt
     4081 tgctccagtt cggtgaaata aagtggcagg accttttcaa gacctagaag aattccatcc
     4141 gttgatccaa tcgttttctc aaccagtccc gctttttcaa attttttagc tactgcattg
     4201 aatttaaagg ctactacaga ctcaagattg tcatcattgg aaaaaataag atgagtttgt
     4261 ggagtaaatt cattattcgt taaaaagtct tggaaaacgg gttttacttc atttaaaaat
     4321 gaattcaatt gagctttcac ttgagaaccc atggcgcctg aaacattgct ggaagaatca
     4381 aagacgtagg cgaccttatc attttcaaag acttgcacag ccaaaaataa ataaatcgac
     4441 aaggttaata aggggatcac cgttaagagt aataatattt tatatcttat tgatatgccg
     4501 gagtttttca caatcttgag tatgcctctc catttgaagc ctgtgtaggt ctagccttta
     4561 gatcttgggc gaaggtccat attttttttt caataagttg ccgaaatgat tgtgaatgaa
     4621 tgcgttcacg agatttacat tgttagatcg gatgatttta agtggatcaa tgatgattct
     4681 cttagtgtta tcttattttc tttatgatga ctctcttctt ttcccttccg aattacaaaa
     4741 gcaaactccc atagggatca tcgggtcttc taataaagat gtgcgcaaaa aaaacataaa
     4801 ggattttgtc tggcttccag gaaatcaaaa agaccaagtt tacgaacaag acagtatctt
     4861 cactggggat gactcccagg cctcaattca attgagtgat gggtctgtca ttaaaatcga
     4921 agaaaactct ctggtaaatc taaataccaa aaatggccag atgcaattgg acttgcgatt
     4981 tggtcaattc gccggaaacg gtaaaacccc tctgcatatt aaaactggca atgaagaata
     5041 caccatccaa ggaaatgacg cacaatttga aatcaatcgc tcgaaaaccg gagttatgga
     5101 tgtaaaggtt ctttctggca ccgctgaaat cagcagtaaa gtgggaaaac aaaaattagt
     5161 tgccaatgaa tcgctcaaaa tttcaaaaca caaaactgaa aaaaatgagg tcgatagtga
     5221 ggtcgaggcg aaaattcacc tcttaactaa gaacgatctc cttgtaatcc gtggcgacaa
     5281 aaccagcact gacaaactgc caatctcttt tgaatggaaa agccaaggtc ctctggttca
     5341 atatcaaatt gagatagccg acacggaaga ttttataaaa atacttactc tccagtcaac
     5401 tgcagagcgg aaaattactt taaaagattc gttcaaagag ggagcttatt tctggcgtgt
     5461 caaaggcctg gatatcaaac agcaaacatt ggcgatctca gaaaaacata aattctctct
     5521 gagagatctg gcggctccaa gaattatgac accggacaat caaagcacta ttactaaaaa
     5581 aataattccc tcagataaag aactgcaaac taacactcaa attgcctggg aggccgacgc
     5641 atctatcgaa agcttcgaat ggcaacttag taaaactgcg gagtttacag aaattataaa
     5701 tgaaaagagc ttgcgtggaa aatccctgac caccccagat ctaggagagg gaacttattt
     5761 cactcgtgtc cgtggcttcg ataaaagtaa aaatccttct caatggtccg aggtgcgtgt
     5821 ttttaatttt gcatttcaaa cagagccaaa gccacccgct ccgattttag cgcaaaaaca
     5881 catccgtttt gccataccta aactggaagg ccgagcgccc tctaccgcga cctctccgca
     5941 aatggcatgg tcggctgtgg atcacataaa aacatatcat tgggagatcg ccaacaatcc
     6001 gcgtttcacc ggagctcaag ccgccgatac tccagcgaca aaagccgtct ggactcagta
     6061 taagcctgga aaattttatt tccgtgtctt tgctcgcagt g
//
LOCUS       DATBTE010000065         4595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_75566, whole genome shotgun sequence.
ACCESSION   DATBTE010000065 DATBTE010000000
VERSION     DATBTE010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75566"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="VIG33_02585"
     CDS             complement(<1..1235)
                     /locus_tag="VIG33_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8269747.1"
                     /translation="MSSQDFLNYIDGEFSPAQSKSHFNKLNPYTGETLGNVAASDAMD
                     VIRAIQAAKKAQGEFEKWTLEQRAELLTAIALKLEEKLDTFALQEALHQGLSFQFVKE
                     KSLQYSIHQFRAAAEKVQEFRHKKDSAFYAQPTGVVSLILSWNLSLRLLAERLAPGLA
                     AGNVCLVKVSEFSPVTAMIMGEVLTAVQAPKGLVQFIQGRGAEVGALLAAHPSIRGVS
                     FIGKLAHAEKVSQGALPQFKKIQIHSGVKNNIFVLNEVDFQNKMPEIVQSFLRGQGQL
                     CWNTTRLFVLESFQKEFIEKLTEYLSSLKPAASPEDSSPWTPLICEALLNEMEKKSQQ
                     VKLEEGKLLCGGIRHSGPGFFFPPTVSLDLPNCSELQQEDVCGPLLILTAVKYQHEML
                     KWANIGPYGHSAVVWAPSI"
     gene            complement(1275..2840)
                     /locus_tag="VIG33_02590"
     CDS             complement(1275..2840)
                     /locus_tag="VIG33_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEY8269748.1"
                     /translation="MHKYYLFPGKLAAFKEETVISTLLGSCVAVALHDPIAKIGGLNH
                     YLLPEPGPNDVGNPRYGSVAIEMLIEEMLRLGASLSHLQAKVYGGGNVIAVSILGESI
                     GQRNIEMAEQMLKNRGIRIVEKNVGGESGRTLKLNTNTFEVLHNFSNEGAAGSASVDV
                     SGFKPLQVAKSVKVLIVDDSATVRTLFTNIFQKSGLQVVGAAADAYQARELIASQKPD
                     VLTLDIEMPRMSGVMFLEKLMKFQPTPVVMVSSLGSNGEAAMKALELGAVEFVHKPSQ
                     FDPQILKELAGFLVEKVRAAASVNMLKRLKEKPPVFSSDETTPAVGKVKKKNQELKVV
                     VMGGNAGSASAVEKVLSGLAIDSPPVVVSCSIVTNFLESFFQKMKGKVKVSLVAAKDG
                     DWLRMGTVYFIPAEHHGKIVNSTHGPQIKIEKGAPVASQLPSANVLFKSAAQAYGGGV
                     YGILLSGFGTDGVEGLTAIQDKGGMTVVQHPEDAEFPYGPQKAIELGVEEQMIDADGI
                     AKHLMQYRNQTVF"
     gene            complement(2833..3612)
                     /locus_tag="VIG33_02595"
     CDS             complement(2833..3612)
                     /locus_tag="VIG33_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY8269749.1"
                     /translation="MSALALDEFTLEMRLHYIETTLESLERFKNHTDFFIEVSAVIDE
                     WISEGLSNKMNFFVNLLEGLKSEIVNGPFLKKEDGIRVAKILSEYLQVLKTEADSEIH
                     FKKFRAVFMQDGDEAEEEKIQFYLKCVSHTHHFIVPVQNVIEIVSGKKIFPLPLTQPG
                     IYGLISFRGQGIPVVNLEDLGFKANVDSENGKTCYVVCDYKESFFALKVHSTDDVIEL
                     APSQFQKCSESSLSSPVVDRFVIREDKSLMLLDIEKMVRHA"
     gene            complement(3609..>4595)
                     /locus_tag="VIG33_02600"
     CDS             complement(3609..>4595)
                     /locus_tag="VIG33_02600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8269750.1"
                     /translation="SLKEAFSGLFPLVRQAAYELKKDVCFKDTGFDLEIDKVLAADLY
                     KALMHMVRNSIDHGIGTPEERTQVGKNPQGILQIKVEEKSSVIHCEIQDDGKGMDKNR
                     ILQRALEKGLTNSLEASQMSEEKIFSFIFHPGFSTKETITTISGRGVGMDVVQNIVAK
                     YGGQIAVTSKIGEGSTIHLSIPVPRKLLVERCLLGEWHHYSLAFPLSEVSHICSCGEL
                     QLTAVDELRFCQFDGKTVPLLTIEEIYEFKTNLNISQVKAMSVIFLKSQSFYLGVLVD
                     KIQHQADLVIKSFGNLIKPSPGFKGNSVLADEQIAFVVDSAELQRLLQRKEM"
BASE COUNT         1303 a         1157 c          882 g         1253 t
ORIGIN      
        1 tcaatcgaag gcgcccagac cacagcagag tggccatagg gtcctatgtt cgcccacttt
       61 agcatttcgt gttgatattt cacagcggtc aaaatcaata aaggcccaca cacatcctct
      121 tgctggagct ctgaacagtt cggcaaatcc aaactcactg ttggtggaaa gaaaaaacct
      181 gggccagaat gacgtattcc tccgcacaaa agttttcctt cttcgagctt cacctgttgt
      241 gatttctttt ccatctcatt gaggagcgct tcacaaatta gcggagtcca aggactagaa
      301 tcctcaggcg acgccgctgg cttgagggaa ctcaaatact cggttaattt ttcaatgaat
      361 tctttttgaa aagactccaa tacgaacaga cgcgtcgtat tccaacaaag ctgtccctgg
      421 cctcttaaaa agctttgaac aatttctggc attttgtttt gaaaatccac ctcattcaaa
      481 acaaaaatat tattttttac tccggagtga atttggattt ttttaaattg tggtaaagcg
      541 ccttggctca ctttttcagc gtgagccaat ttacctataa agctgactcc gcgaatagaa
      601 gggtgagccg ctagcaaagc gcccacttcc gcgccacgtc cttgaatgaa ttgaaccaat
      661 cccttaggag cttgaacagc ggtcaaaact tcacccatga tcattgccgt cacgggagag
      721 aactctgaaa ctttcacgag acaaacattt cccgccgcaa gcccgggtgc caaacgctct
      781 gccaacaggc gcagcgacag attccaagaa agaatcaaag aaaccacacc tgtgggctga
      841 gcgtaaaaag cggagtcttt tttatgcctg aactcctgaa ccttttcagc agccgcacgg
      901 aattggtgta tagaatattg aaggcttttt tctttaacga attgaaaaga caacccttga
      961 tgaagggcct cttgcaaagc aaaagtatca agtttctctt ccagtttgag cgctatcgcc
     1021 gtcaacaatt ccgctctttg ctctaaagtc cacttttcaa attcgccttg agcttttttg
     1081 gccgcttgaa tagcgcgaat cacatccata gcatctgaag ccgctacatt ccctaaagtt
     1141 tcccctgtgt aagggttcag tttattgaaa tgtgatttgc tttgggctgg agaaaattct
     1201 ccatcgatat aattaagaaa atcttgagag ctcatggccc tcaagatcca gcaaaatgat
     1261 ttgaaaagca agccctagaa aacagtctga ttgcgatatt gcatcaggtg cttggcaata
     1321 ccatcggcat cgatcatttg ctcttcaaca cccagttcga tagctttttg cggaccataa
     1381 gggaactccg catcctctgg atgctgaacc actgtcattc cacctttgtc ctggatagcc
     1441 gtcaagccct caactccatc agttccaaag ccagagagaa gaataccata aaccccgcct
     1501 ccataagctt gcgccgcgga tttgaagagc acattggccg aaggaagttg cgaagccacc
     1561 ggtgcgccct tttcaatttt gatttgagga ccatgggtgg aattgacaat cttgccatgg
     1621 tgctcagcag gaatgaaata caccgttccc attcgcaacc aatccccatc cttggccgcc
     1681 actaaactca ctttaacttt gcccttcatc ttttgaaaaa aactttccaa aaaattagtc
     1741 acaatactgc aggaaaccac taccggtgga ctgtctatcg caagaccgga caaaactttc
     1801 tctacggcac tggcacttcc tgcattgccc cccatcacga ccactttgag ttcttgattc
     1861 ttctttttca ctttgccgac ggcaggggtt gtttcgtcgc tggaaaatac aggaggtttt
     1921 tccttgagtc tcttaagcat atttaccgag gccgccgcac gaactttttc caccaggaag
     1981 cccgccaact cttttaagat ctgaggatcg aattgtgatg gtttatgcac aaattcaaca
     2041 gcccccagct ccagggcttt catcgcggct tccccgttgc tcccaaggga agaaaccatg
     2101 accaccggag ttggttggaa cttcatcaac ttttctaaaa acatgacacc actcattctc
     2161 ggcatttcaa tatccagagt caacacatcc ggcttctgag aggcaatcag ctcacgggcc
     2221 tgataggcat ccgccgccgc tcccacgacc tgaaggccgc ttttttgaaa aatattagta
     2281 aaaagagttc tcactgtggc agagtcatcc acgattaaaa ctttcacact cttagccact
     2341 tgcagaggtt taaagccaga cacatccacg gaggcagatc ccgcagcccc ttcgttgcta
     2401 aaattatgca ggacttcgaa agtattagta ttcaatttaa gtgtgcgtcc agactctccg
     2461 cctacattct tctctacaat acgaatacct ctatttttta acatctgttc agccatttcg
     2521 atattacgct gaccaatact ctctccgaga atagagaccg cgatgacatt gcctccgccg
     2581 taaacttttg cctgaagatg actcaaagag gcccccaagc ggagcatttc ttcaatcagc
     2641 atttcaatcg ctacactgcc ataacgagga tttcccacat cgttggggcc aggctccgga
     2701 agtaagtaat gattgaggcc gcctatcttc gcgatgggat cgtgcagagc cacagccaca
     2761 caggatccta agagagtgga aatcactgtt tcctctttaa aagccgcaag ttttcctgga
     2821 aataaatagt acttatgcat gtcttaccat tttctcgata tctaaaagca tcagagattt
     2881 atcctctctg atcacaaaac gatccaccac gggagatgac aaagaggact ccgagcattt
     2941 ttgaaattgc gaaggagcca actcgatgac atcgtccgta ctatgaactt tcagagcgaa
     3001 aaatgattct ttataatcac agacgacata acaagttttt ccattctccg agtccacatt
     3061 tgctttaaaa cccagatcct ctaaattcac cactggaatg ccttggccac ggaaacttat
     3121 aaggccgtaa atgcctggct gggtcaaagg caaaggaaat atttttttac cacttacaat
     3181 ctcgatcaca ttttgcacag gcactatgaa atgatgggtg tgacttacgc attttaaata
     3241 gaattggatc ttttcttctt cagcttcatc tccgtcttgc atgaacacag cacgaaattt
     3301 tttaaagtgg atttccgaat ccgcttcagt tttcaacact tgtaaatact cgctgagaat
     3361 cttcgcaacc cgtattccat cttctttctt caaaaaaggg ccgttgacaa tttcggattt
     3421 aagtccctcc aaaagattta caaaaaaatt catcttatta gaaagacctt cagaaatcca
     3481 ctcgtcaata accgcgctca cttctataaa aaaatcggta tggtttttaa aacgctctaa
     3541 agactctaga gtcgtttcta tataatgaag gcgcatttcc agagtaaatt catctaaagc
     3601 cagtgcactc acatttcctt cctttgcaaa aggcgttgca gctctgcgga atcaaccacg
     3661 aaggctattt gctcatccgc tagaacagaa ttccctttaa atccagggga aggttttatg
     3721 agatttccga atgatttaat aaccagatcg gcctgatgtt gaatcttatc cactaagact
     3781 cccaaataaa aactctgtga ttttagaaat atgacagaca tcgccttcac ctgactgata
     3841 tttagattcg ttttaaattc ataaatttct tcaatggtta acaatggcac cgtcttaccg
     3901 tcaaactggc aaaagcgaag ttcatccact gcggtcaact gcaactcccc gcaagagcaa
     3961 atatgagaaa cctcggacaa cggaaatgcc aaagagtaat gatgccactc ccccagaaga
     4021 caacgctcaa ccaaaagttt tctgggaacc ggaatactta aatgaattgt cgagccttca
     4081 ccgattttcg acgtcaccgc aatctggcca ccatatttag cgacaatgtt ttgcacgaca
     4141 tccataccca ccccacgacc ggaaatggtg gtaattgttt ctttagtgga aaatccggga
     4201 tgaaaaataa aagaaaatat tttttcttcg ctcatctgcg aagcctcaag ggagttggtg
     4261 agtccctttt caagcgcacg ttgaagaatg cgattcttat ccatcccttt gccatcatct
     4321 tggatttcac aatgaataac cgaagatttc tcttccactt ttatttgcaa aatcccttga
     4381 gggttctttc cgacttgcgt tcgctcctct ggcgtgccaa ttccatggtc gatggaattt
     4441 ctcaccatat gcatcagggc tttataaaga tccgcagcta aaaccttgtc aatctcgagg
     4501 tcaaaaccgg tatctttaaa acatacatct tttttgagtt cataggccgc ttggcgtacc
     4561 agtgggaaca gaccggaaaa ggcttctttc aaaga
//
LOCUS       DATBTE010000066         3390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1646666, whole genome shotgun sequence.
ACCESSION   DATBTE010000066 DATBTE010000000
VERSION     DATBTE010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646666"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="VIG33_02605"
     CDS             <1..1378
                     /locus_tag="VIG33_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269751.1"
                     /translation="AIQSEDLFMYLAIAREYFKTGSFPVHDPFLYSIPNFTWTILHQW
                     LGYLAFYGLYELGGYSLIIIVKTVLITGVLCVPLLRARKSSPATLIWGTSTLIAVFAM
                     NFRFMERTSLFSDFFIVITLNILMSEQVRPGRWKYLLPALFVFWVNLHPGFPTGWLLC
                     GVFLLINFKKWKSAEYRIFFGLTLISILVCLFNPRGLEGVLYPFNFMKNEGAVYRKFY
                     FEWMPTLHPFYREHAQTYFIIGLILLNLVLIYQSRKSKPIFEIIVSGFFIFYGLYAIR
                     FVPSFCFALVALNTSLALKLSYAGFFKNRISYLNGALAALALILAGKNMIWGYETISG
                     HREFGLGLDEHVVPYRAAQLLDQSGLTDKVYNSHLFGSYLAWAWEGRRKVIYHGFVTD
                     TDFFLKEFRIFSANRAQFDTQADKFQIGAFLVDRFQGNESLLTILSQHPSWQLVYKDE
                     GSLIFVKK"
     gene            1394..2413
                     /locus_tag="VIG33_02610"
     CDS             1394..2413
                     /locus_tag="VIG33_02610"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEY8269752.1"
                     /translation="MKNLKVFLSDSLNPYLNLATEEWIFHNLDPAQQVLFLWRNEETV
                     VIGRNQNPWTECNLAQMKNDNIHLARRTTGGGAVFHDLGNTNFTFLSPRESYRRENNI
                     EIIFQALRVFGIKGEVSVRNDLLIPFHDGPRKFSGSAYREKKDRAFHHGTLLLHADLG
                     RLGQYLTPNPKKLQGKGKESVRSRVVNLTEIVGHLEHKKIVESMIGSFEKFYDAKAEI
                     VSLDLESLPKIPELKEQYDLLTSWNWRYGNTLEFDHKMDEYLSLGFFDVHFKVDEGYI
                     TDLKIFTDCLYPQLIEDLTEALKRQAYCGDSIRTVFAAINAKHTELAVELAELEQWLC
                     RHIEI"
     gene            2543..3172
                     /locus_tag="VIG33_02615"
     CDS             2543..3172
                     /locus_tag="VIG33_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HEY8269753.1"
                     /translation="MSHYERKYYISGDKVGSILSELSARYAHTDIYPCSIVDSIYYDT
                     LNLDFFRQCNDGSSTKTKVRIRRYNKDPFQQVQVKKKRGFEVIKQKWEVEGLDPFNPL
                     CRQKVMQTSGIQGLQATTQIIYKRHRFRVEDMRITFDSNIQFQSMPGYVSVRKFGRLP
                     FSVLEIKSSNPDSQITLLRDWGLSVNSFSKYYLAQAFFFNRADVFNKFF"
     gene            3193..>3390
                     /locus_tag="VIG33_02620"
     CDS             3193..>3390
                     /locus_tag="VIG33_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269754.1"
                     /translation="MKGLDFLATQTDFTAMSYVKMFIALGAAGIFGLSIMMLYRLYHR
                     PNETYDLSISRSFPIITPAVTM"
BASE COUNT          893 a          666 c          740 g         1091 t
ORIGIN      
        1 tgccatccaa tctgaagatc ttttcatgta tttggctatc gcgcgtgaat actttaagac
       61 gggttcgttt ccagtccatg atccttttct ttattccata cccaacttta cttggactat
      121 cttacatcag tggctgggat atttggcttt ttatggcctc tatgaactgg gtgggtactc
      181 gctgattatt attgtgaaaa ctgttttgat cactggggta ttatgcgtac ccttattaag
      241 ggcgaggaag tcttcaccag ctactttgat ctggggaacc tccactttga ttgctgtatt
      301 tgcaatgaat tttcgtttta tggaacgaac ttctttattt tcggattttt ttatcgtcat
      361 cactctgaat attttgatga gtgaacaagt tcgtcctggg cgatggaaat atcttttacc
      421 agccctcttt gttttttggg tgaatctgca tccgggattt ccgacgggtt ggcttctctg
      481 cggtgtattt cttttaataa attttaaaaa atggaagtct gcggagtatc gcatattttt
      541 tggactcacc ctaatatcca tactggtttg cttgttcaat ccgcgggggc tcgagggcgt
      601 gctgtacccc tttaatttta tgaaaaatga aggtgcggtt tatcgcaagt tttattttga
      661 gtggatgccg actttacatc ctttttatcg tgaacatgca caaacctatt ttatcattgg
      721 tttgatactg ctaaatcttg ttttgattta tcaatcgaga aaatcaaaac ctatttttga
      781 aataattgta tctggatttt ttatttttta tggactttac gcgattcgtt ttgttccttc
      841 tttttgcttt gcccttgtcg ccctcaatac atctttggcg ttgaagcttt cttatgcagg
      901 tttttttaaa aatagaattt cttatttaaa tggggcgctg gccgcgctgg cgctgattct
      961 agcgggcaaa aatatgattt ggggttatga gaccatctct ggccatcggg aattcggact
     1021 gggccttgat gaacatgtag ttccttatcg cgcggcacag cttttggatc agtccgggtt
     1081 gacggataag gtctataact cgcatttgtt tggcagctat ttagcttggg cctgggaagg
     1141 tcggcgtaaa gttatttatc acggctttgt cacggacacg gatttctttt taaaagaatt
     1201 taggattttt tctgcaaata gggcacagtt tgatacccag gcggacaaat tccagatagg
     1261 tgccttttta gtagaccgtt ttcaaggaaa tgagagttta ctgacaatac tgtcgcaaca
     1321 tccaagctgg cagctggttt acaaggatga gggcagcttg atctttgtta aaaaatagta
     1381 gtagattcat gccatgaaaa atctcaaagt tttcctgtct gacagcctca atccctatct
     1441 taatttagcc acggaagagt ggatttttca caacttggac cctgctcaac aagttttgtt
     1501 tctgtggcgc aatgaggaga cggtggtgat aggtcgtaac caaaatcctt ggacggagtg
     1561 caaccttgcc cagatgaaaa acgacaatat ccatttagcg cgcagaacaa ccgggggcgg
     1621 tgcagttttt catgatctgg gcaacacgaa ctttactttc ctttctccgc gggagtctta
     1681 tagacgggaa aataatattg aaattatttt tcaagccctg agagtgtttg gtatcaaagg
     1741 tgaggtttca gtccgcaatg atttattaat tccttttcac gatgggccac ggaaattcag
     1801 cggcagcgcc tatcgtgaga aaaaagaccg agccttccac catggtactt tgcttttgca
     1861 tgcggacttg ggtcgtttgg gacaatactt gactcccaat ccaaaaaagc ttcaaggcaa
     1921 gggtaaagaa tcggtgcgtt caagagtggt gaatttaacg gaaattgttg gccatctcga
     1981 gcacaagaaa attgttgagt cgatgattgg ctcttttgaa aaattctacg acgctaaagc
     2041 tgaaattgta tctctggatc ttgaaagctt gccaaagatt ccggagctta aggaacagta
     2101 tgatttatta acctcctgga attggcgcta tggcaatact ctcgaatttg accacaagat
     2161 ggatgagtat ctcagccttg gttttttcga tgttcatttc aaagttgatg aagggtatat
     2221 tacagacttg aaaattttta cggactgtct ttatccgcaa cttatcgagg atttgacgga
     2281 agccttgaag cggcaagcct attgcggaga ttccatccgc actgtctttg ccgcgatcaa
     2341 tgccaagcat actgaactgg cggtagaact tgcagagttg gagcagtggc tctgtcgaca
     2401 tattgagatt tagtacggat aaattgcggt aaagatagta tatctattga tattgatgaa
     2461 tgttgtagtt aaccttcgga gtaaaaataa ctttagctca ttgagctctc caagcacatg
     2521 cagttttttg agaggttacg acttgtcaca ttatgaacgt aagtattata tttccggcga
     2581 taaagtaggt tctattttgt ccgaactttc agcccgatac gctcacaccg atatttaccc
     2641 atgcagcatt gtggactcta tttactacga taccctaaac ctggactttt ttcgccagtg
     2701 taatgacggt tctagtacaa aaaccaaggt gcgaatcaga cgctacaata aggatccttt
     2761 tcagcaagtt caagtgaaga aaaaaagagg tttcgaagtc atcaaacaaa aatgggaagt
     2821 ggaaggcctc gatcccttca atcccttgtg caggcaaaaa gtaatgcaaa cttctggtat
     2881 tcaggggctg caagcaacca ctcagattat ctataaacgg catagattcc gagtggaaga
     2941 tatgcgcatc acttttgatt ccaatattca atttcaaagt atgccagggt atgtctcagt
     3001 gagaaaattt ggacggctgc ctttttcagt tctcgaaata aaaagttcaa atcctgactc
     3061 ccaaatcact ctattaagag attggggctt gtcagtcaac tcgttctcaa aatactatct
     3121 ggcacaggct ttctttttta atcgagcgga tgtttttaat aaattttttt aaaactcaac
     3181 aattggagaa gtatgaaggg cttagatttt ttagcaacac aaacagattt cacggccatg
     3241 tcttacgtga agatgttcat tgccctggga gccgctggca tatttgggct ttccataatg
     3301 atgctttatc gcctttacca tcgtcccaat gaaacctacg acctttccat ttcgcggagc
     3361 tttccgatta taacacccgc agtcaccatg
//
LOCUS       DATBTE010000067         5257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1646745, whole genome shotgun sequence.
ACCESSION   DATBTE010000067 DATBTE010000000
VERSION     DATBTE010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646745"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="VIG33_02625"
     CDS             complement(<1..1158)
                     /locus_tag="VIG33_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEY8269755.1"
                     /translation="MKNTAQKKILTLLALAFTLLTVGAHLYLNKHHIDLKLGVSSVNS
                     ICNVSQTLNCDTAAASPFAEFFGIPMALLGALTNGLLLFFLLLNRYNLTENTERMERY
                     SFYLSSFILAVSIFMASISLFVLKSACPFCLATYALSLLTWLTLLTAYRPSLRLLMDD
                     VSDTFTTEKWILGCLIAVPVLGMVVNNMTLDSYGYEEIRRVSESSLSTWQSSPHQNFN
                     LESGLGFQNSQKEPKAVIVEFADFLCSHCKAAYPVLHNFAKSHSDVKLIFKSFPLDGV
                     CNSAVTHKGDGKRCELSYATLCAEKIHQKGWIAHHYIFDNQETIFSKPMEEVLNDISK
                     ETGIDRAQMKACMDTKEIHETVKKMASEGEKVQINGTPAIFLNGKNLPGGQL"
     gene            1234..2865
                     /locus_tag="VIG33_02630"
     CDS             1234..2865
                     /locus_tag="VIG33_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEY8269756.1"
                     /translation="MLTDLPKNLQNWIRIRKELYRQMEAQVSIRLGGEDAELIKYRKE
                     YNKEFKKKWASSSREELYRRLQTSRLVLMGDFHALSQSQKTHWRVLKNFITKRPLVLC
                     LECFEAEDQIILDKFMTGKISEREFLKNIEWSRKWGFPWDYFRPLIRWAQGKKIPLYG
                     INRSVKSRTAASLKSRDVFAAQKLHLILKKHPEAIVFAIYGDLHLAVNHIPKEVVKLE
                     GPGFRKKIFRLFQNSEKIYFQLLEQELEGNVDVVELTENAFCFLNVPPWVKWQNYLMY
                     LEQTYDVGLEDQEEVVDYTDHISNYVKIISEELGKSLSCDDLSVYTAQDQQFWTQLQQ
                     HFNTKEMKWIEALIEDENSFYLPSLGIGYLARPTVNHAATLAAKYVHAKWSGSTRSFL
                     DMPKDFLRQIWVEGLAYFGSKMINHKRKTDTVMDIRTALASRGTAGSDKESLMLALSQ
                     KMHELMVISNRPQGRPVIQPKKKSSYLVASTLLGGMMGERLYSAYRKGLMPKVTLLSF
                     LKRPLMEDSFNIAYYEIMEIIESLPAPFRSKKEKM"
     gene            2862..3284
                     /locus_tag="VIG33_02635"
     CDS             2862..3284
                     /locus_tag="VIG33_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HEY8269757.1"
                     /translation="MSSWYFNKNLKPQGPLSFEEMKKKIMRGEVGPYDLICKEAEGGW
                     SQAMQWRDFPKELFPAFQKNYFKNSDLSEKEWILLSFKLDHGSGLQEGPYSVEDVIAL
                     LASGAVSCEDYIWRSGLSGWVRIQDRREFASQALHKDP"
     gene            3366..5078
                     /gene="argS"
                     /locus_tag="VIG33_02640"
     CDS             3366..5078
                     /gene="argS"
                     /locus_tag="VIG33_02640"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY8269758.1"
                     /translation="MIQTAKSQIAYFLQPLTQLPLEDLQKTLERPKQFEHGHIAFPVF
                     ALAKTLRAAPPQIAADLAKKIQESIQSGRLPFLEKVQAVGGFLNFTFKNSYLQKILFE
                     SLLKPNVGFSEFGKNKTVIIDYASPNVAKPMHVGHLRAAMVGQAIRNLAESQGFKTIG
                     VNHLGDWGSQFGKLAWAYKSWGHEYTFDVPMDDLVRLYVRFHEAVEKNPELELAAAAN
                     FKKLEDGDPEIKKIWKMIVAHSLKDYDRLLNGVLKIKHDAVLGESFYNDKMDHVIQLL
                     RQKNLLQESEGAQVVFFEEKENMPPCIIKKSDGASIYATRDLAAAIYRHEVMKGDELL
                     YVVGQDQTLHFRQVFKVLEMLGFEWVQACHHISFGLYRFKEGKMSTRAGRVVLFEDVI
                     NQAIELVEQTIAEKNPHLENKSVVARQVALGAVIFNDLVNDRVKNVEFDWDRILSVEG
                     DSGPYVQYTVVRCKSILRKYGKEILTEAPLELTGEEERKLIYQLLQFEDILTGAYRQF
                     KPNILAQYLLELCGSFSHFYHKCRILGESAEVEASRITLVAATEKILIQGLKLLNIEA
                     PEAM"
BASE COUNT         1517 a          951 c         1284 g         1505 t
ORIGIN      
        1 aagttgtccc ccaggaagat tttttccgtt caagaaaatc gctggggttc cgttaatttg
       61 aactttttcc ccctcagaag ccatcttttt aactgtttcg tggatttctt tggtatccat
      121 gcaggccttc atttgcgcgc ggtcgatgcc agtttcttta ctgatatcgt tcaaaacctc
      181 ttccatgggt ttagagaaaa tagtttcttg gttatcgaag atatagtgat gcgcgatcca
      241 gcccttttga tggatctttt ccgcgcagag cgtggcatag cttaattcgc agcgttttcc
      301 atcgccttta tgagttaccg ctgagttgca cactccatcg agaggaaaac tcttaaagat
      361 taatttcaca tccgaatgcg atttagcaaa gttatggaga acaggatagg ctgctttaca
      421 atgtgaacaa agaaaatccg caaattcaac aatgacggcc ttaggttctt tctggctatt
      481 ttggaatcca agaccgcttt ccagattaaa attttggtga ggagaacttt gccaggtact
      541 gaggctgctt tcactgactc ttctaatctc ttcgtaacca tagctatcca aagtcatatt
      601 atttaccacc atccccaaaa cgggaacggc gatgagacaa ccgaggatcc atttttcggt
      661 ggtaaaggta tcgcttacgt catccataag aagccgaaga ctcgggcgat aagcggtcag
      721 aagagtgagc caggtaagca gagaaagtgc gtaggtggcc aaacaaaaag ggcaagcact
      781 tttaaggacg aataaagaga tggaggccat gaatatggac acggcaagaa taaaacttga
      841 aagataaaag ctgtagcgct ccatgcgttc cgtattttct gtcagattgt agcgattcaa
      901 aagaagaaaa aacaaaagga gaccattggt aagagctcca agaagagcca tagggatgcc
      961 aaagaactca gcgaagggac tggccgcagc agtgtcgcaa tttaaagttt ggctaacatt
     1021 gcaaatgcta tttacggaac tgacaccgag tttgaggtca atatgatgtt tgttaaggta
     1081 aagatgcgcc ccaacggtca agagagtgaa agcaagggcg agaagagtca ggatcttttt
     1141 ttgcgctgta tttttcatct ttccgattag accccacaat gtggcttgaa atcaatggct
     1201 tttatgcaaa aataaacact tgcgttggag tgaatgttga cagatttgcc taagaattta
     1261 caaaattgga ttcgtatccg taaagagctt tatcgccaaa tggaggccca ggtatcaatt
     1321 cgtcttgggg gtgaggatgc tgagctcatc aaatatcgta aagaatacaa taaagagttc
     1381 aaaaagaaat gggcgagttc cagtcgggag gagctttatc gccgactgca aacgtcgcgt
     1441 ttggtgctga tgggcgactt tcatgctctt tctcagtcac aaaaaactca ttggcgagtg
     1501 cttaagaact tcataacaaa gcgtccattg gttttatgtt tagaatgttt tgaagcagag
     1561 gatcaaatca ttcttgataa attcatgact ggaaaaattt cggagcgtga gtttttaaaa
     1621 aacattgaat ggtccagaaa gtggggcttt ccctgggatt attttcgccc tctgattcgc
     1681 tgggctcagg gtaaaaagat tccgctctat gggattaatc gctcggtgaa gagtcggacg
     1741 gcagcgagcc tgaaatctcg agatgtcttt gccgcccaaa aactccattt gattctgaaa
     1801 aagcatcctg aagccattgt ctttgcaatt tatggtgatc ttcatttggc ggtaaatcat
     1861 attccaaaag aagtggtgaa gttggaaggt cccggatttc gtaaaaaaat cttccgtctt
     1921 tttcaaaact cagaaaaaat ttactttcag ctcttagagc aagaattaga gggcaacgtt
     1981 gatgtcgttg aactcacgga aaacgctttt tgctttttaa atgttccgcc ctgggtgaag
     2041 tggcaaaatt acttaatgta ccttgagcag acctatgatg ttggcttgga agatcaagaa
     2101 gaggtcgtgg attacacgga tcacataagt aattacgtga agattatttc tgaagagttg
     2161 ggaaaatctt tatcctgtga tgatctttct gtttacacgg cgcaggatca acagttttgg
     2221 actcaattac aacaacattt taatacgaaa gagatgaagt ggattgaagc tttgattgag
     2281 gatgagaact ccttttacct gccgagcctt ggaattggtt atctggctcg tccaacggtg
     2341 aaccacgcag ccaccttggc cgcgaagtac gttcacgcga aatggagcgg aagtacccgg
     2401 agctttttgg atatgccaaa ggatttttta agacagattt gggttgaagg tctggcttac
     2461 tttggttcaa agatgatcaa tcataagcga aaaacagata ctgtgatgga tatccgtacg
     2521 gctttggcct caagggggac cgctggaagt gataaagaat ctttgatgct ggcgctttct
     2581 caaaaaatgc atgaattgat ggtgatttcc aatcgccctc aaggtcggcc tgtgattcag
     2641 cccaaaaaga aaagtagtta tttggtggcc agtactctgt taggggggat gatgggggag
     2701 cgtctttatt ctgcttatcg caaaggattg atgccaaaag tcacgcttct cagttttttg
     2761 aaaaggccgt tgatggaaga cagttttaat attgcttatt atgagattat ggaaatcatt
     2821 gaatcattgc cagcaccatt tcgcagtaaa aaggagaaaa tgtgagttct tggtatttca
     2881 ataaaaatct aaagccacag gggcctctta gttttgaaga aatgaaaaag aaaatcatgc
     2941 gaggggaagt gggtccctat gatttaattt gcaaggaggc agaggggggt tggtcacaag
     3001 caatgcaatg gcgagatttt cctaaagaat tatttcccgc ctttcagaaa aattatttta
     3061 aaaattcaga tctgtcggaa aaagaatgga ttttgctgag tttcaaactg gatcatgggt
     3121 caggccttca agagggaccc tattcggtgg aagatgtgat tgcgttgctc gcatcggggg
     3181 cggtgagttg tgaggattat atttggcgaa gcggtctttc gggatgggtt cggattcagg
     3241 atcggaggga gttcgcgagc caggctcttc ataaagaccc gtaagcgctc aaagctctga
     3301 ctaaaatact tactaagtcg ctggatttac attggaattg gtagggattt ttggtagttt
     3361 ttggcatgat tcaaactgct aaatcccaaa ttgcttattt tttacaacct ttgacccaac
     3421 ttcccttgga agacttacaa aaaacacttg agcgaccaaa acaatttgag cacgggcata
     3481 tcgcttttcc ggtttttgca ttagccaaaa cactgcgtgc ggctccgcct caaattgcgg
     3541 cggatttagc taaaaaaatt caagaaagta ttcaaagtgg aaggctcccc tttttggaaa
     3601 aagtccaagc ggtgggtgga tttctaaatt tcacatttaa aaatagctat cttcagaaaa
     3661 ttcttttcga aagtcttttg aagcccaatg tgggtttttc tgaattcggt aagaataaaa
     3721 ccgtgatcat cgactatgcc tctccgaatg tggcgaaacc catgcatgta ggacatttgc
     3781 gagcggcgat ggtggggcaa gccattcgca atttagctga gtctcagggg tttaaaacga
     3841 tcggtgtcaa tcaccttggt gattggggga gtcagtttgg aaagctggct tgggcttata
     3901 agtcctgggg tcatgaatac accttcgatg tgccgatgga tgatctggta aggctttatg
     3961 tcagatttca cgaggcagtg gaaaaaaatc ctgagttgga gctcgcagcg gcggcaaatt
     4021 ttaaaaaact cgaagatgga gacccagaga ttaaaaaaat ctggaaaatg atagttgctc
     4081 attccttaaa agattatgat cgactcttaa atggagtact gaaaattaag catgatgccg
     4141 tgttagggga gtctttttat aatgataaaa tggatcatgt gatacagttg ctgaggcaaa
     4201 agaacctctt gcaagagagt gagggcgctc aggttgtttt ctttgaggaa aaagaaaata
     4261 tgcctccttg tattattaaa aaaagtgatg gcgcttccat atacgccact cgggatttgg
     4321 cggcggcgat ttatcgtcat gaggttatga agggagatga attgttgtat gtggttggcc
     4381 aggatcagac tttgcatttt aggcaagtct ttaaagtttt agaaatgttg ggctttgaat
     4441 gggtccaggc gtgtcatcat atttcattcg gactctatcg tttcaaagaa ggaaagatgt
     4501 cgacccgtgc gggacgagtg gtgctttttg aagatgtgat taatcaggcc atcgaattgg
     4561 tggaacaaac cattgccgaa aaaaatcccc atcttgaaaa taagtccgtc gtggccagac
     4621 aggtggcttt gggggctgtc attttcaatg acttagtgaa tgatcgtgtg aaaaacgtcg
     4681 aatttgattg ggatcgtatt ctcagcgttg agggcgatag cggtccttat gttcaatata
     4741 cggtcgttcg ttgtaaaagt attttaagga aatatggaaa agagattctt accgaagctc
     4801 ctctggaatt gactggggaa gaagaaagaa aactgattta tcagcttttg cagtttgaag
     4861 atatcctcac cggtgcgtat cgccagttta agccgaatat tctggctcaa tatctcttag
     4921 aactttgtgg aagcttcagt cacttctacc ataagtgtcg catcctaggc gaaagtgcgg
     4981 aagtggaagc ttctcgtatt accttggttg cggcaactga gaagattttg attcagggcc
     5041 ttaaacttct gaatattgag gcgccggagg ctatgtagga gtgattctca ttttgagact
     5101 gtctcaactt ttttagaaaa tcaccgataa gaaacttatg aaaagagttt ctaattcttt
     5161 attcgtgata gtttgtttga gcattaccgt aggtctcttg tggagttact gcgagttcac
     5221 gaattacttc aatcaaggaa aagaatatca agttcaa
//
LOCUS       DATBTE010000068         5052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1031807, whole genome shotgun sequence.
ACCESSION   DATBTE010000068 DATBTE010000000
VERSION     DATBTE010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031807"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            802..4017
                     /locus_tag="VIG33_02645"
     CDS             802..4017
                     /locus_tag="VIG33_02645"
                     /inference="COORDINATES: protein motif:HMM:NF020044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEY8269759.1"
                     /translation="MSNIWAKAYRNTFEPMVQNRGRAYFQSGRVSLEFLENNVAKFKV
                     QGTRPYQVEINHHLEDRKLECTCSCPYFATKTPCKHIWAAILQTDKNELFEIKGFVGS
                     EDIEVTPSWKLGLLNAQQRLRSQIKFEDLTAAKAVDQAQYCGVYALDLDRSREHKRLY
                     LQILTQVYPPKIPPIPPKIRELSIGSIVQFPEDSDQEILQDLLGKTELYGVYDNLDKE
                     KRFSTVVLPKDHAVEVLQKIGKSEKLFRLPSKKMFYSKNSPVEVDPYEYKDQFWGLQL
                     ELKKIDENYYLMPFLRCQAETKPLREVEWAFGEFIFFQYFMAKSDVARHSLWVEFFGK
                     NKEVEILAEELNDFLEYYFHQSSMPMLRLPSDLVFSDLEDVQPVPRVSLTALKPAQFG
                     VGVEFIYGDQEASMENRNEFIYNVETKQKIRRHAEFEHQVVNEIQGFGLQRPSRVMEE
                     SFDFIFRESQFVKVAEQILAKKWQLLVAQRPVTLGGIERIEVQSSGVDWFSLQVDFEF
                     AGQKFGLPQLLGQLQTGSRVIELGDGSTGILPEEWFRKFAPLIEMGQLKDDKLHINKL
                     QALFVSAQLEGHEKFQADNRFRSLTELFIELQNLKPQESGEKFLGQLRPYQQKGLAWL
                     DLISQEGIGGILADDMGLGKTIQIIALFVAHQAKVGTAKTKRPHLIVAPKSLVFNWIK
                     EIEKFAPHLKSLDFTGKTRVANIKDIKKYDVILTTYQTLRMNIERLQGQEFEFFVMDE
                     AHYIKNAEAQSSKACRLVKAKKKLALTGTPVENSLMDLFSILEVVSPGLITREVRARY
                     SREKDVAALNDLGKALRPFVLRRTKDEVLTDLPEKSEQILYCELSASERTKYDELKKH
                     YWTQLSGTIEDKTFERSKFEVLEALLRLRQAACHQGLLNAEMQDESSSKFDLLLDQLS
                     LVIQDGHKALIFSQFTSLLALLKPQLEARGIRYEYLDGQTRDRQERVERFQDNPEISL
                     FLLSLKAGGVGLNLTSADYVFILDPWWNPAAESQAIDRAHRIGQKQKVFAYKIIAKDT
                     VEEKILELQKSKKQLAKAIVSGGDQGLLKNMSMDDLRLLLG"
     gene            4109..>5052
                     /locus_tag="VIG33_02650"
     CDS             4109..>5052
                     /locus_tag="VIG33_02650"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEY8269760.1"
                     /translation="MKVIEHIQNSKETLFSYEIVPPPRGRSVKDIIDVVEQLAPLNPP
                     WIDVTSHSSTTDFQERVDGTIHKRTLRKRPGTLGICGIIQNRFRIDTVAHMLCLGFTR
                     EETEDALIELSFLGIENVLALRGDAPNFQKKIRTDRTTNEFAKDLVVQIRDLAEGSFL
                     DELDKAAPLRFGVGVAGYPEKHFEAASLKLDIQHLKKKVDAGADYIVTQMFFDNQKYF
                     DFVKQCRAAGIEVPIVPGLKVLKSIPQLKSVPRNFYIDFPDALVDEATSNPQHVGEIG
                     KRWAEKQVTELLKFGVPAVHFYVLNDVQSVVEVVKKFK"
BASE COUNT         1394 a         1032 c         1240 g         1386 t
ORIGIN      
        1 tcatccaggg ctacttactt tgaaaactat ttggtaggtt aaaagttttt tatggaataa
       61 atttgggatc gcaagcggtg ttgagagaaa tttcccgaca tccaataatc agcgcatcat
      121 tttttattta aacggaaata accttgacag cgattttata aaaaatcttt tttcattatt
      181 tctactttat aagtctcctt aaattagcta tagcaagggc taagcggcct ctaaaaggct
      241 ctgcttcttg agtgcaagag ggtgaatttg tgggctcaga aggacatcgt tgtaccagtc
      301 gttttcaact agaaatcgat cacctgtatc cttggagtca aggcggtagc aacagggttg
      361 agaacttatc gctgaaatgc aaatctcata atttctaccg cacccaagag acccacggat
      421 ttttctacaa ggctcagaag taagtaaatt ccaagcaacc caagtaaacc ccagaacccc
      481 cgagaagttt ctttttccct cgaagttctg gccacgatgg ccagaactca gcgttcccat
      541 ggacggaggc ccttcgcgca acctatcgac agctggacga agacgagaac atctcctatt
      601 cctattcgca ttcttccttt ccgcattcca taaccccgcc gacgcttaac ccgcaaattc
      661 ttcagacgag ccgttcaacc tgccatctat cctaatattt aatattcaac ctaaatatta
      721 gtccaacttt gatttttagc ctcacgaaag gcctgacaaa gggaattatt tcgtgtaggg
      781 tgagcgattg gagaaatttt gatgagtaat atctgggcaa aagcttatag gaacacattt
      841 gagcccatgg tgcaaaaccg tggcagagca tattttcagt ctggacgcgt tagtcttgag
      901 tttctcgaga acaatgtagc gaagttcaag gttcagggaa cccgtcctta tcaggtggag
      961 attaatcatc atcttgagga tcgcaaactc gaatgcactt gctcctgtcc ctatttcgca
     1021 accaagaccc cttgcaaaca catttgggcc gccattcttc aaactgacaa aaatgaactt
     1081 ttcgaaatca agggcttcgt tggttctgaa gatatcgaag tcactcccag ttggaaatta
     1141 ggactgttga atgctcaaca gcgtctgcgt tcccaaataa aattcgaaga cctcaccgca
     1201 gcaaaggctg tggatcaagc tcaatactgc ggggtctatg ccttggatct tgatcgcagc
     1261 cgcgaacata aaagacttta tttgcagatt ttgacccaag tctatccgcc aaaaattcct
     1321 cctattccac caaaaatcag agaactgtcg attggctcca ttgttcaatt tcctgaagat
     1381 tcagatcaag agattttgca agatttgcta ggtaagacgg agctttacgg ggtgtatgat
     1441 aatctcgata aagagaaacg gtttagtaca gtggttttgc caaaggacca tgccgttgaa
     1501 gttttgcaaa agatcggcaa aagtgaaaaa ctttttcgat tgcccagcaa gaaaatgttt
     1561 tactcgaaga acagccccgt ggaagtggat ccctatgaat acaaggacca gttttgggga
     1621 ctgcaacttg agcttaagaa aattgacgag aattattacc ttatgccttt tcttcgctgc
     1681 caagcggaaa ccaaaccact gagagaggtg gagtgggcct ttggcgaatt tatttttttc
     1741 cagtatttta tggcgaagtc cgatgtggct aggcactctc tctgggtgga gtttttcgga
     1801 aaaaacaaag aagtcgaaat ccttgctgaa gaattgaatg atttccttga atattatttc
     1861 catcaaagtt cgatgccgat gttgcgtttg ccttctgatt tggtctttag cgatttggag
     1921 gatgtacaac cagttccgcg ggtgtccttg acggcattga aaccggccca gttcggagtg
     1981 ggcgttgagt ttatctatgg cgatcaagaa gcctccatgg aaaaccgcaa tgaattcatc
     2041 tacaacgtcg aaacgaaaca gaagatccgt cgccatgcgg agtttgaaca ccaagtcgtg
     2101 aatgagattc agggctttgg cttgcagagg ccttcacggg ttatggaaga aagctttgat
     2161 tttattttca gggaaagcca atttgtaaaa gtggctgagc agattttggc taaaaaatgg
     2221 cagctgttag tagctcagcg gccagtgact ctgggaggca ttgagcgtat cgaagttcag
     2281 tcctcagggg tggactggtt tagcctgcag gtggattttg agtttgccgg tcaaaagttt
     2341 ggccttccgc aacttttggg ccaattgcaa acgggctcac gagtcattga gcttggagat
     2401 ggcagtacgg gaattttgcc cgaggaatgg tttcgtaaat ttgctccgct tatagagatg
     2461 ggccaactca aagacgataa actgcacatc aataagctcc aggctttatt cgtcagcgcg
     2521 cagctggaag gccatgagaa gttccaagcc gacaatcgct ttaggtctct gactgaattg
     2581 tttattgagc tgcaaaatct caaacctcaa gagagtggtg agaagttcct gggtcagctt
     2641 cgaccttatc aacaaaaagg tttggcatgg ttggatttga tttcccaaga gggcatcgga
     2701 ggaattctcg ctgatgacat gggtttaggg aaaaccattc aaattatcgc gctctttgta
     2761 gcgcatcagg cgaaggtagg gacggcaaag actaagagac ctcatttaat tgtggcaccg
     2821 aagagtttgg tgtttaactg gatcaaagag atcgagaagt tcgcgcctca tttgaagtcc
     2881 ttggatttca ctggcaaaac ccgcgttgcc aatattaaag atatcaaaaa atatgatgtg
     2941 atcttaacca cctatcagac tcttcgtatg aacattgagc gactgcaagg ccaggagttt
     3001 gaattctttg tgatggatga agcccattat attaagaatg ccgaggctca gtcctcaaaa
     3061 gcttgtcggt tagtcaaagc gaaaaagaag ttggccctga cggggacacc ggtggaaaat
     3121 tctttgatgg atttattttc catccttgaa gtcgtcagcc caggcctcat cacccgcgag
     3181 gttcgagcga ggtatagccg agagaaggat gtcgccgccc tgaacgattt aggaaaagcg
     3241 ctccgtccct ttgttcttcg ccgcacgaag gatgaggtgc ttacggattt accagagaag
     3301 tctgagcaga ttctttattg tgaactcagt gccagcgaac gtactaagta tgatgaactc
     3361 aagaaacatt attggactca attgagtggt acgattgagg ataagacttt tgagcgttcc
     3421 aagtttgagg ttttagaggc tttgttaagg ctccgccaag cggcctgtca tcaagggctt
     3481 ttaaacgccg aaatgcagga tgaaagttca agtaagtttg atttactttt ggatcagttg
     3541 agcttagtga ttcaagatgg ccataaggct ttgattttct cgcagttcac cagtttattg
     3601 gctttgttaa agcctcagtt ggaggcccgg ggcattcgtt atgaatatct cgatggtcaa
     3661 accagggatc gtcaagagag agtggagcgc ttccaggaca atccggaaat ttcgctcttc
     3721 ttgttgagtc ttaaagcggg gggagtgggc ttgaatttga cctctgcgga ctatgttttt
     3781 attttagatc cctggtggaa tcccgcagcc gagtctcagg cgattgaccg agctcatagg
     3841 attggccaaa agcaaaaggt ctttgcctat aagatcattg cgaaggatac ggtcgaggaa
     3901 aagatcctgg agctgcaaaa gagtaagaaa caattggcga aagccattgt ttcgggcgga
     3961 gatcaaggtt tgctgaagaa tatgagtatg gacgacttgc gacttttgct tggttaatta
     4021 agccgattag ataaaaatag aaaaagagac acagaaaact cccgatagac agggaaatgc
     4081 aaattgaatg cagattggga cctcagaaat gaaagttatt gagcacattc aaaattctaa
     4141 agagacttta tttagttatg aaatcgtacc gcctccgcgc ggtcgttcag tgaaagatat
     4201 tatcgatgtg gtggagcaac tggctccttt gaatcctccc tggatcgatg tgacctcgca
     4261 cagttccacg acggatttcc aagaacgtgt ggatggcacg attcacaagc gaactttgcg
     4321 caagcgccct gggactctgg gaatctgcgg aattatccaa aatcgtttcc gcatcgatac
     4381 tgtggcccat atgttatgtc tgggctttac gcgagaagaa accgaagatg cattgatcga
     4441 gttgagtttt ctggggattg agaatgtttt ggctctgcgc ggggatgcac cgaattttca
     4501 aaagaaaatt cggaccgatc gcacgacgaa tgaatttgcc aaggatcttg tggtgcagat
     4561 tcgagatctt gctgaaggtt catttttgga tgaactcgac aaggcggctc ctttgcgctt
     4621 tggcgtggga gtggcgggat atcctgagaa acattttgag gctgccagtt taaagttgga
     4681 cattcagcat ttgaaaaaga aagtggatgc gggcgccgat tacatcgtga ctcagatgtt
     4741 tttcgataat caaaaatatt ttgatttcgt gaagcaatgc cgcgcggctg gtatcgaggt
     4801 tcccattgtt ccgggtctta aagttttaaa atcgattccg cagttgaagt cggttcctcg
     4861 caatttttat atcgattttc cggatgctct ggtggatgaa gctacgtcaa atccacaaca
     4921 cgtcggcgag attggcaagc gctgggcgga aaagcaagtg accgaacttt taaaatttgg
     4981 ggttcctgcg gttcattttt acgtcctcaa tgatgttcag tccgtggtcg aagtggttaa
     5041 aaagtttaaa ta
//
LOCUS       DATBTE010000069         1767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1304883, whole genome shotgun sequence.
ACCESSION   DATBTE010000069 DATBTE010000000
VERSION     DATBTE010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304883"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VIG33_02655"
     CDS             <1..472
                     /locus_tag="VIG33_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269761.1"
                     /translation="VLLPFNLEFRYERDASQQTVDRRPKNFAIGAGTVASTILFEYSA
                     FNENTGNKTLSLARTHQEYSFWWKEKFMNLEFLDAFFSGGVGGYEEKVTTTLSGAGSA
                     TDSSGLQALAGASVGLQSLMFNYVLVSIEGRLIAGKNFDPNPQGGILLRLGAEF"
     gene            complement(668..>1767)
                     /locus_tag="VIG33_02660"
     CDS             complement(668..>1767)
                     /locus_tag="VIG33_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269762.1"
                     /translation="NNSSERILKILQDKAPTLAPYFKDFRDLVFNTDYSKTKVWESTP
                     FGLVELDDQNITSLIPENCKKEGKTQLIQAVIREFQSFSGADIGHIIYKFDPKIVDSL
                     DKKSPLQLSFLMVHEWLWDLSQNVDRNRRINRFLHSREIESMSSEKVIKTLQGMGLTI
                     PDVQADLFDDQVCQGTPMTAQDLDTHYPGAYVLASWGQISIQQRQRVLNCPAPLKNCD
                     TAWKDMVNSNLLFQDNYKYFLSPTWVDNNRKFPLKIISPQYMNHIGQSVRYDRGQFAC
                     RFTEDPKQNIECKIIDPIFYSPLFGSSPEPFQEMPLLKGILTTECFRLKATGNTKIEI
                     FGNVQSGEGQVRETETVFYLRAKDGVFLKPH"
BASE COUNT          467 a          351 c          400 g          549 t
ORIGIN      
        1 agtgcttttg cctttcaact tagagtttcg gtatgagcga gatgcttcac aacagacggt
       61 agatcgtcga cccaagaatt ttgcaatagg tgccggtacg gtcgcttcga cgattttgtt
      121 tgaatattcc gctttcaacg agaataccgg aaataagact ctttcactag cacgcactca
      181 tcaagaatat agtttttggt ggaaggaaaa attcatgaac ttagaattct tggatgcttt
      241 tttcagcggc ggagtcggtg gttacgaaga aaaggtaacg acgactttat caggagccgg
      301 aagtgccacc gactcgagtg gcttacaggc gttggcaggt gctagcgttg gtcttcagtc
      361 gttgatgttt aactatgttc ttgtaagcat tgaaggtcgc cttattgcgg ggaaaaactt
      421 tgaccccaac ccacaagggg gcattttact gcgcctggga gcggagtttt aaataatttt
      481 taatttccag cagtgacccg ggtcttgaac ttacgttatc agataaacca ccaatctgta
      541 ttttaatact tttccttgat cttttagaat gatcattggc tgttttaaac cgaaggagac
      601 acctcggttc agatattgga ttcttttttc ttccattatc ctgatgtttt cgaaccatga
      661 gttttactta gtgaggtttt aaaaacactc cgtctttagc gcggagataa aacactgttt
      721 cagtttccct gacttggcct tcgccggatt gtacattgcc gaatatttct attttggtat
      781 ttcctgtcgc cttgagccgg aagcactcgg tggtgagaat gcccttgaga agcggcatct
      841 cctggaatgg ttcaggcgag cttccaaaga ggggagaata gaaaattgga tctatgattt
      901 tacattctat attttgcttt ggatcctcag taaatcgaca ggcgaattgg cctctgtcat
      961 aacgtacact ttgacctatg tggttcatgt attgcgggct aataattttt aacggaaatt
     1021 ttcgattgtt atcaacccaa gttggagaaa gaaaatactt atagttgtct tgaaaaagta
     1081 aattcgaatt gaccatgtct ttccaggccg tatcgcagtt ctttaaagga gcagggcagt
     1141 tgagaactcg ctggcgttgt tgaattgaaa tctgtcccca gcttgccaac acataggcgc
     1201 ctggatagtg agtgtccagg tcctgcgccg tcatgggggt gccctggcaa acttgatcat
     1261 caaatagatc tgcttgaaca tcgggaatcg tgagacccat gccctgcaaa gttttgatca
     1321 ccttttctga agacatcgac tcaatttcac gagagtgaag aaagcgatta atgcgtcgat
     1381 tgcgatctac attttggctt aaatcccata gccattcatg aaccattaaa aaagataatt
     1441 gcaggggtga ttttttatcc aacgagtcca ctatttttgg atcaaattta tagatgatat
     1501 ggccaatatc ggctccggaa aagctttgga attcacggat cactgcctga atgagctgag
     1561 tctttccctc ttttttacaa ttctctggaa tcaaagaagt gatattttga tcgtccaatt
     1621 cgactaggcc aaagggagtt gattcccaca cttttgtttt gctatagtcc gtattaaaaa
     1681 ccagatcgcg aaagtcttta aaatagggag ctaacgttgg ggccttgtct tgcaggattt
     1741 ttaagatacg ttctgaagaa ttattcc
//
LOCUS       DATBTE010000070         6858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_621842, whole genome shotgun sequence.
ACCESSION   DATBTE010000070 DATBTE010000000
VERSION     DATBTE010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621842"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VIG33_02665"
     CDS             <1..910
                     /locus_tag="VIG33_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269763.1"
                     /translation="FSLPLLKLEVEAPLKDNNEIFLQLESAEYRDANSNRFDTQLKEG
                     YLSLTSMLSARAELRYGLIPDFYIELQREQWGYDFWGATSSLPLLKYKYTSWSDLGGM
                     YQAELPGDWGHWALSITNGEGMQSDEIGPRKQAQLVVGLAKAAPFYVMASYVHGGYEN
                     YDESFNKKTRLLVHMSYEFSKALVALEYYGTHDPANAILAGGIASGVDVSALQATNVE
                     GQGVALFSYMSLTENINLFLWADWLSPVKQEKEKNLKALSTGLSYEASEDIHWALAYE
                     YTDYSDTFAAAIRDQSQLVLATRVIF"
     gene            1073..1723
                     /gene="phoU"
                     /locus_tag="VIG33_02670"
     CDS             1073..1723
                     /gene="phoU"
                     /locus_tag="VIG33_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY8269764.1"
                     /translation="MDSQIEELKRLILVMGGYVERALTEVTQALMSRNADNFAQIHDI
                     EKKINEGHIAVDNACLAVFAKQGPVAKDLRLILSIVKINTDLERMGDQCVNIAYTGKD
                     YLARKPLAEVADISRMSNIVCVMVKESLDCFVRGDVELAKKILLMDDEVDGLKNKVFK
                     DNLNHMKNVSSDVEACLDLILVARNLERLGDHATNIAEDVIFAFTGKDVRHGGKYA"
     gene            1704..2432
                     /locus_tag="VIG33_02675"
     CDS             1704..2432
                     /locus_tag="VIG33_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8269765.1"
                     /translation="MVGSTHEFSATVLVVEDEQEIRELMALHLLRQGFKVKECASAEE
                     ALKEFQGEPQKRKFDLVILDWMLPGVSGFDFLHQIKQNSGTPYVLMVTAKTEPQDIVS
                     GLEGGADDYLTKPFDPGVFLARVRALLRRTKAQEIPSTVDLDKFHLEGLSVNFKTYEV
                     KLRDEQLHLTPSEFKLLGNLLESQGRVLTRDQLIENIQGEGINVTGRTIDTHVFGLRK
                     KLGAWSDNIETIRGVGYRMKMDSQ"
     gene            2576..3871
                     /locus_tag="VIG33_02680"
     CDS             2576..3871
                     /locus_tag="VIG33_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8269766.1"
                     /translation="MQYFLATLPLAALISWRFSRPLHKIILKAFRVASKKMENQSEDE
                     EDLLDEEMGEYAQIENALNRIHRKMKKRRDQLQQEREETQAFMCAVQEGIVSVSPDLR
                     VLYFNSQFATQFLDSKQMNVSLTLADIFRYPEIHEGFLRALKEGRTFKVQVKLETKLE
                     KNIRYFLVSMNPLRKSKSNEVYGAMGIFHDITELKKAELVRIEFVGNASHELRTPLTS
                     IKGYVDTLKSDVHSGYYEQAGKFIDIISRNVDRLADLVNDLLSISALESNAEIKIESV
                     NPLQVSEHIVAELAQFSSEKNQVIMVVGAQELPNFNADISKVEQVLRNLVSNAVKYIP
                     SGKTIQIRWQKTDNGDITLRVIDDGPGIPPEHLGRLFERFYRIDKGRSRDAGGTGLGL
                     AIVKHIMQSHGGGVQVKSEVGRGAEFICTFPFKKISFKS"
     gene            3895..4776
                     /locus_tag="VIG33_02685"
     CDS             3895..4776
                     /locus_tag="VIG33_02685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEY8269767.1"
                     /translation="MAVSFYQYFEKIYGSRWGALFDALTTPERQVARLNLLSNAEPVI
                     LQDAAPLHPSLHHCYWIPQQAYFQPERTSEDLLNVYIMDPASVIVARAMEVQEGDRIL
                     DMCAAPGGKTLILIEGIKKFGELLANDLSSERRDRLKKVIQQYVPRNIRDRVWVTGKD
                     GVQFGLKESNSFDRVLLDAPCSGERHVLENKKAMEEWGPRRGEHLSIRQYSLLSASLL
                     AVKPGGRIVYSTCTINPEENDGVIHKLLKKKKDQVRLLPQETYSVGEKTEYGISFLPD
                     RCGFGPLYYSVIEKTAF"
     gene            complement(4787..5059)
                     /locus_tag="VIG33_02690"
     CDS             complement(4787..5059)
                     /locus_tag="VIG33_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269768.1"
                     /translation="MTTDLLFQIFHQHLHDPDCQIESATELIQKIVGTYLLFISRTGN
                     IPDGHLQDVIEDLQAEVVEMYRKKTYGHFDLKDYREHLKKKLDEST"
     gene            5094..6389
                     /locus_tag="VIG33_02695"
     CDS             5094..6389
                     /locus_tag="VIG33_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF814 domain-containing protein"
                     /protein_id="HEY8269769.1"
                     /translation="MKAMSLLELKTLVTELEKLLVDTQLQDVISNDRGLVLGFRGKTH
                     FWLTFDMNPPNPFCLVFAGNCPFKKGPKPKPVALFLNSHGKNLYFQRVWLEEGYGRVL
                     KIELGNSQKNCVLKAILIPRQPNLLVEAEDKKIAWEKPRELQVQSEPGPFPETRDLQQ
                     IHKDWLTEFQGTGASAGVRPSLDPQAQWEKKQAKDLQKKRKALEEIEKTLAENHSEKF
                     YELGNYLKALVPSDFSARQLPSQWHDLVDFKKNIFVNIEIIFEKAKQAASKVAGTQSR
                     KLIVMDEIAQLEKMTFESSQRPGAGRAKVDDLMKNIEAKGRKLNLSSGAIVYCGKSGA
                     DNLALLRKAKAWDYWLHLKDYPGAHAIIHRARDQELPFEEIQKAALWVLKESFSSKTL
                     MPGEKYAVVLVECRFVRPIKGDKLGRVTYHSEKQFMVLS"
     gene            6443..>6858
                     /locus_tag="VIG33_02700"
     CDS             6443..>6858
                     /locus_tag="VIG33_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8269770.1"
                     /translation="MIEVRDLTKDYGPRRAINKLNFTINKGEVVGLLGPNGAGKSTTM
                     KIITGFMAPSSGLATIAGFDVFESPLEVKKRIGYLPETPPIYTDMYVRDYLTYVAELK
                     QVPQDKIKTQVQGALEKTNLLGVEKRLIHNLSKGYR"
BASE COUNT         1900 a         1320 c         1680 g         1958 t
ORIGIN      
        1 tttcagtctt cctttgttga aacttgaggt tgaagctcct ttgaaagata ataacgaaat
       61 ttttttgcaa cttgaatccg ctgaatatcg cgatgccaac tctaaccgtt tcgacactca
      121 acttaaagag ggttacttga gcctgacaag tatgctgtcg gctcgagcgg agcttcgata
      181 cgggctcatc ccagattttt atatcgaatt acaacgagag cagtggggtt acgacttctg
      241 gggagcgacc agtagcctgc ctttacttaa gtataaatac acttcatggt ccgatttagg
      301 gggcatgtat caagctgagc ttcctggcga ctgggggcat tgggctttaa gtattacaaa
      361 tggtgaggga atgcaaagtg acgagatcgg tcctcgtaaa caggcgcaac tggtagttgg
      421 gcttgcaaaa gcagcccctt tttatgtcat ggcttcttat gtccatggtg gatatgaaaa
      481 ctatgatgaa agttttaata aaaaaacgcg cttgttggtc catatgagtt atgaattttc
      541 taaagccctt gtagcgcttg aatactatgg aactcatgac cctgccaatg ccattctcgc
      601 cggcggtata gcaagcggag ttgatgtttc ggctctccag gcgaccaatg tggaagggca
      661 aggggtggct ctgtttagct acatgagtct cactgaaaat ataaatctct ttttatgggc
      721 ggattggctg agtcccgtaa aacaagagaa agaaaagaat ttgaaagctc tttctacagg
      781 gctttcctat gaggctagcg aagacataca ttgggctttg gcctatgaat atacagatta
      841 tagtgatacg tttgccgccg ccattcgaga ccagagtcag ctcgttttag cgactcgagt
      901 gattttttaa ggcgaaaatg ttaagatttt gttagaatta tatgaggaat attttctgag
      961 cgacttgacg cgggttattt gaagaaaatg aaatgtcgtc agtttcggag tctatttgct
     1021 ttttaaaatc aatttctgta aaaaatgaca gggagtcaga gtggaaagaa taatggattc
     1081 tcaaatagag gaactcaaac ggcttatctt agtgatggga ggctatgttg agagagcact
     1141 cactgaagtc acgcaggccc ttatgtctcg caatgcggat aactttgcac agattcatga
     1201 tattgaaaag aagattaatg agggtcacat agctgttgat aatgcctgct tggcggtttt
     1261 tgccaaacag ggtcctgttg ccaaggatct gcgcttgatt ctttccatag ttaaaattaa
     1321 tacggatctc gaacggatgg gggatcagtg tgtaaatatc gcttacacag gtaaggatta
     1381 tttggctcgg aagcctcttg cagaggttgc ggatatcagc cgcatgtcca acattgtttg
     1441 tgtcatggtt aaggaatcct tggattgctt tgtgcggggt gatgttgagt tggccaaaaa
     1501 gattttgtta atggacgatg aagtggatgg tcttaaaaac aaggttttta aagataatct
     1561 taatcacatg aagaatgtct cttcggacgt agaggcttgt ttagatctga tccttgtggc
     1621 ccgtaatctc gagcgcctcg gagatcatgc gacaaatatc gccgaagatg ttatttttgc
     1681 gtttacaggt aaggatgtta gacatggtgg gaagtacgca tgaatttagt gccacagttt
     1741 tagtggtgga agatgaacag gaaattcgtg aactcatggc tttacattta ctgcgccagg
     1801 ggttcaaggt taaagaatgt gcttccgcag aagaggcgct gaaagaattt cagggcgaac
     1861 ctcaaaagag aaaatttgat ctggtcattt tggattggat gcttcccggt gtcagtggct
     1921 tcgattttct ccaccaaatc aagcagaact caggaacccc ttatgttttg atggtgacgg
     1981 cgaagaccga gccgcaagat atcgtcagcg gactcgaagg tggtgctgat gattatctca
     2041 ctaaaccttt tgatcccgga gttttcttag ctagagtgcg tgctctttta agaagaacca
     2101 aggcgcagga aattccgtcc actgtcgatc tggataagtt ccatcttgag ggactgagtg
     2161 tgaatttcaa aacttatgaa gtgaaattgc gtgatgaaca gcttcatttg actccttctg
     2221 aatttaagct tcttggcaat cttctggaaa gtcaggggcg ggtcttaacc cgtgatcaat
     2281 tgattgaaaa tattcaaggc gagggtatta acgtcaccgg tcgcactatt gacactcatg
     2341 tttttgggtt gcgtaaaaaa ttaggggctt ggagcgataa tattgaaacc attcgcggag
     2401 tgggttaccg catgaaaatg gattctcaat gaggaattgg gctcgcttcc catggaagct
     2461 tttttggcaa atttatttta tacagatttt tctctataat tttctttttg ttcttactct
     2521 cataattatc agtaggtcgc atccttttgg gtcgacctat ttttggtcca ttatcatgca
     2581 gtatttttta gcgactttgc ccttggcggc tctgatttcc tggaggtttt ctagaccact
     2641 tcataaaatc attctaaaag ccttccgtgt cgctagcaag aaaatggaaa atcagagtga
     2701 ggatgaagaa gatcttttag atgaagaaat gggggagtat gcccagattg agaacgctct
     2761 caatcgaatc catcgtaaga tgaaaaaacg tcgtgatcag cttcagcaag agcgggaaga
     2821 aacccaagcc ttcatgtgcg cggttcaaga gggaattgtc agtgtcagcc ctgaccttcg
     2881 agtgctttat tttaactcgc agtttgcgac ccagttctta gattcaaagc agatgaatgt
     2941 ttctttgacc ttggcggata ttttccgtta tccagaaatt cacgaaggtt ttttgagagc
     3001 tttgaaggaa ggacgtactt tcaaagttca agtaaagttg gaaaccaagc ttgaaaagaa
     3061 tatccgatat ttcctggtgt cgatgaaccc gctacgtaaa agtaaaagca acgaagttta
     3121 cggggccatg ggaatttttc atgatatcac cgagcttaaa aaagccgagc ttgtgcgtat
     3181 tgaatttgtg ggaaatgcct ctcatgagtt gagaacgcct ctgacctcca tcaagggata
     3241 tgttgatacg ctcaagtctg acgttcatag cggttattat gagcaagcag ggaaatttat
     3301 tgatattatt tcccgcaatg tggatcgact ggcagatttg gtgaacgatc tgctgagtat
     3361 ttccgctctc gaatcaaatg cggaaattaa aattgaatcg gtaaatccct tacaggtttc
     3421 agaacatatc gttgctgaac tggcgcaatt ctcgagtgag aagaaccagg tcatcatggt
     3481 ggtcggagcc caggaattac ctaattttaa tgccgacatt agcaaagtgg aacaagttct
     3541 cagaaatctc gtgtcgaatg ccgtgaagta tattccttca ggaaagacta ttcagattcg
     3601 ctggcagaaa acggacaatg gggatattac tcttcgggtg attgatgatg ggcctgggat
     3661 tccgcccgag catttggggc gtctttttga aagattctat cgtatcgaca agggtcgttc
     3721 ccgcgatgcg ggaggcacag gtctgggctt agccattgta aaacatatta tgcaaagcca
     3781 cgggggtggc gttcaggtaa agagtgaagt gggcaggggc gctgagttta tttgtacttt
     3841 tcccttcaag aagatttcat ttaagtctta ataaatgaaa cagggacgta agttatggct
     3901 gtgtcatttt atcagtattt tgaaaaaatc tatggttcgc gttggggggc tctttttgat
     3961 gcactcacaa cacccgagcg acaagtggcc agattgaatc ttctcagcaa tgctgaacct
     4021 gtgattttac aggacgccgc tcctttgcat ccgtcgctgc accattgtta ttggattcca
     4081 caacaggctt attttcaacc tgaacgcacc tccgaagatc ttcttaatgt ttacattatg
     4141 gatccggcaa gtgtcattgt tgcccgtgcg atggaagtcc aggagggcga ccgtattttg
     4201 gatatgtgtg cggcaccggg ggggaaaacc ctgattctaa ttgaaggaat taaaaaattc
     4261 ggagagcttc tcgctaatga cctttcatcg gagcgtcggg atcgactcaa gaaagtgatt
     4321 cagcaatatg tgccacgcaa catccgtgac cgtgtttggg tgactggcaa agacggtgtg
     4381 cagtttggac ttaaggagtc gaacagtttt gatcgagtct tattggatgc gccttgctcc
     4441 ggtgagcgcc atgtattgga aaataagaag gccatggaag agtggggacc ccgtcgtggt
     4501 gaacatcttt ccatccgtca atattcactt ttgagtgcct ctcttttagc agtcaagccc
     4561 ggtggaagaa tcgtctactc aacctgcaca atcaatccgg aggaaaatga tggcgtgatt
     4621 cacaagctgc tgaagaagaa aaaagaccaa gtgcgtttgc ttccgcagga gacttattcg
     4681 gtgggtgaga aaactgaata cggtatttct tttttgccag accgctgtgg atttggacca
     4741 ttgtattatt cagtgatcga aaagacggct ttttaagaac ttaggtttaa gtcgactcat
     4801 ccaatttttt tttaagatgc tcgcggtaat ccttgagatc gaagtgtccg taggtctttt
     4861 tacggtacat ttctacgact tcagcttgca agtcctcgat gacatcttga agatgaccat
     4921 caggaatatt tcctgtacgc gaaataaata gtagataagt tcctacgatt ttttggataa
     4981 gctccgtggc agattcgatt tgacaatcgg gatcatgaag gtgctgatgg aagatttgaa
     5041 aaagtaaatc tgttgtcata tattctttta tcggcaagct gatgggaaac tgaatgaaag
     5101 caatgagttt attagagctt aagactttgg tcactgagtt ggaaaaactc ttagtggata
     5161 cccaattgca ggatgtgatc tctaatgatc gcggtttggt cctgggcttt cgtgggaaga
     5221 ctcacttttg gcttactttt gatatgaatc ccccaaaccc cttttgtctg gtctttgccg
     5281 ggaactgtcc ttttaaaaaa ggtcctaaac ccaaacccgt ggctctattt ttgaattccc
     5341 atggaaaaaa tctgtacttc cagagagtat ggcttgaaga aggctatggc cgagttttaa
     5401 aaattgaact tggaaactct caaaaaaatt gtgttttgaa agcgattctt attccacgac
     5461 aacccaatct tttagtcgag gcggaggata aaaaaatcgc ctgggaaaag ccccgagagc
     5521 tgcaggttca aagcgagccg ggcccttttc cagaaacgcg agatttacag caaattcata
     5581 aagattggct gacagaattc caaggcacag gagcctccgc tggagttcgt ccttcattag
     5641 acccccaagc tcaatgggaa aagaagcagg ccaaagatct tcagaaaaaa cgcaaagctc
     5701 tggaggaaat tgaaaaaact ttggccgaaa atcattcgga gaaattctat gagctgggaa
     5761 attatttgaa agccctggtt ccatcagatt ttagcgcaag gcaattgccg tcgcagtggc
     5821 atgacttggt ggattttaaa aaaaatattt ttgtcaatat agaaattatt tttgaaaaag
     5881 caaagcaagc ggccagtaaa gttgccggca ctcaatcacg taaattgatt gtgatggatg
     5941 aaattgccca gcttgaaaaa atgacttttg agtccagtca acgcccaggg gctgggcgcg
     6001 ctaaagttga tgatttaatg aaaaacattg aagccaaagg acgaaaactc aatcttagct
     6061 ccggcgccat tgtttattgt ggaaaatccg gtgcagataa cttggctttg ctacgcaagg
     6121 ccaaggcgtg ggattattgg ctgcatctca aagactatcc tggggctcat gccattatcc
     6181 atcgtgctcg ggatcaagag cttcccttcg aggagattca aaaggcggca ctttgggttc
     6241 ttaaggagtc tttctcctca aaaactttga tgcccggtga aaagtatgca gtggttctag
     6301 ttgaatgccg ttttgttcgg ccgattaaag gggataagtt gggacgcgtg acttatcatt
     6361 cggaaaaaca atttatggtg ctgtcttaac ttgactggct ctctagtcgg aatagaattt
     6421 cccattattc atgggagaca ttatgatcga agttcgagat cttacaaagg actatggtcc
     6481 ccgccgggcc atcaacaaac tcaatttcac tatcaataag ggagaagtcg tggggctctt
     6541 gggacccaac ggagccggca aatcgacaac catgaaaatc atcactggct tcatggcgcc
     6601 cagctcaggt ctggcaacca tcgccggttt tgatgttttt gaaagtcctt tggaggttaa
     6661 aaaacgcatt ggctatttac cagagactcc accgatctat accgatatgt atgttcgaga
     6721 ttatttgacc tacgtggcgg agctcaagca agttcctcag gataaaatca aaactcaagt
     6781 gcaaggtgcc ttggagaaaa caaatttatt gggggtcgaa aaacgcctga tccacaatct
     6841 ttcaaaaggt tatcgtca
//
LOCUS       DATBTE010000071         4234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_417294, whole genome shotgun sequence.
ACCESSION   DATBTE010000071 DATBTE010000000
VERSION     DATBTE010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417294"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            565..1410
                     /locus_tag="VIG33_02705"
     CDS             565..1410
                     /locus_tag="VIG33_02705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HEY8269771.1"
                     /translation="MQETKEIFRFNDAVDFLNYEFESKRIKNSRFSLRAWARQLGYEN
                     PSFLSHILKKDRSLKIDVAEKFTANLKLTGDAKKYFELLVLLKNSKTVDEKKIYLDIL
                     ESLRPRSEKAPQSLHLDAFRVISDWYHTAILELVELSDFKNDLEWIRARLGNEVSGQN
                     ISKAIQRLLKLELLEETSRGKLGRVKDNPVLLENFIPSDAIRHFHKQMIEKAKSAIEE
                     QDIKERDIRGSMISVRTKDFSKIQEVIRKAHAEIIKYSCAGNGEELYQFNTQFFRITR
                     KKGTV"
     gene            1407..2342
                     /locus_tag="VIG33_02710"
     CDS             1407..2342
                     /locus_tag="VIG33_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269772.1"
                     /translation="MRSIIFLVGAVLSVSMAFASGTTVGNGGDEIRFTFEDARIEAIR
                     IINNIKECSFDSTTSADVRSWILNNSMALASDIQNSAHQWTNEDRPTCAWTQLSARSE
                     IVLSYPSCALNMSTQKAAQLLIHESVHHFGINNESFADAVAVALNQATPGVICSKSTL
                     PENTKWKVRDRLRQMLPPNSKTDSIYFGSYKDTGSYCTVLLSTGEVTDPNKVKIDSFT
                     ISVLEGRFNGSGVGISISVLANTEGSPYLPGYVTFYKDDGDLLSMDYITKYLNQKWEA
                     SSLTVNSINSSITEVTIANKNAPRNPIIKTCILEN"
     gene            2361..2483
                     /locus_tag="VIG33_02715"
     CDS             2361..2483
                     /locus_tag="VIG33_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269773.1"
                     /translation="MKKMFVLALATLTGVNAFGATGKSAKLDTEKKYILVDVTG"
     gene            complement(2594..2884)
                     /locus_tag="VIG33_02720"
     CDS             complement(2594..2884)
                     /locus_tag="VIG33_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269774.1"
                     /translation="MKLYEFTYTDNQNLSEPELLRRVGEFLSKESELQAWAPDYEFRP
                     CRQVEQLAGGEKNYFFEVNGKYLNSDSLEFDQEVSETSPSSRPAAAATDLNP"
     gene            2956..3729
                     /locus_tag="VIG33_02725"
     CDS             2956..3729
                     /locus_tag="VIG33_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269775.1"
                     /translation="MNRQELLEKRGDLSPFLIHLTRSGDLKLDKDIFSLPQDRVVQIT
                     AKESLGSIIGSRRIGARSAFGYFNYKVPLKRADGRVLNSTSHVRRDWLRSVCFTETPL
                     DHVYLQMQTIVGRQLHFESYGLAFRESVVRGANGNPVLYFQTTNQNIRSGFDRLAVDP
                     LAHHFKSMMPLVEGFGPPWFPRYGGPAEIDFRWEREWRITGDFSFTLSDVAFGFCPAD
                     EIAQFETLVGGTFPFVDPLGNLEANKTKLRGWAHLRDLK"
     gene            complement(3730..3912)
                     /locus_tag="VIG33_02730"
     CDS             complement(3730..3912)
                     /locus_tag="VIG33_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269776.1"
                     /translation="MGLDNVIKLALTLTLAAAMTGQLPRITNQIRHAQVKLLEQSKAS
                     KWGSPDLLYSQIGGQR"
BASE COUNT         1153 a          914 c          992 g         1175 t
ORIGIN      
        1 gaaatatcct caaaagacat tttcgccctg gaatatttaa gtatgaatcg aagtgtctca
       61 aactcttggg gatggggcac taggcggccg cgctcgtatt tgaagccgta gggagagttc
      121 caccgaactt ggccagagcc cctttgttct tgagtcttgt tgccctgttt catagatctt
      181 agggaaacgc ctgcgcggat caggatatcg cgaacactgg tacgagggat tccggtgatt
      241 ctggaaatct cgggtaggga gtgcccagac tcatattttg ggacgatctc agtgataatt
      301 ccttgctccg tgagttcagg aatgctaatt aaatcatggg cttgggaaat tcatgccatt
      361 aactcggtcg ggaccccaat ttttacaaca gcagaaaatc aataattcca aacacttaat
      421 gctccgggcc caacgctttt tcttcgaaaa agcgacatga ccattatttc agacgaacca
      481 tctcattcaa cgtgtaccca tgttggatac agtttgtacc caaacgtatt gacctgatga
      541 tagagcctca atacaaatga tctcatgcaa gagaccaaag aaatttttag atttaatgat
      601 gctgtagatt ttttgaacta cgagttcgaa tcaaagcgca ttaagaacag tcgcttttct
      661 ctgcgtgctt gggcgcgcca acttggttat gaaaacccct ctttcctttc tcatatttta
      721 aaaaaagatc gttcacttaa aattgatgtt gcagagaaat tcactgccaa tctcaagcta
      781 accggcgatg cgaaaaagta tttcgaactt ctagtgcttt taaaaaacag caaaactgtc
      841 gatgagaaaa aaatctacct ggacatttta gaatccctcc gccccagatc agagaaggcg
      901 cctcaatctc ttcatctcga tgcgtttcga gtcatttctg attggtacca tacggcgatt
      961 ttggagctgg tggagctgtc cgattttaaa aacgatcttg aatggatcag ggctcgcctc
     1021 gggaatgaag tttcaggaca aaatatcagt aaggcgattc agcgtctttt gaaacttgag
     1081 cttttagaag aaacgagccg tggaaaactt gggcgggtaa aggataatcc tgttttgctc
     1141 gaaaacttca ttcctagcga tgccattcgt cattttcata aacagatgat tgaaaaagca
     1201 aaatccgcca tcgaagaaca agacatcaaa gagcgtgata ttcgtggttc tatgatttca
     1261 gttcgaacga aagatttttc caagattcaa gaggtcatta ggaaagccca cgctgagatt
     1321 attaaatact cctgcgcagg caatggcgaa gagctttatc aattcaacac acaatttttt
     1381 agaattacga gaaagaaagg gacggtatga gatcaatcat ttttctagtg ggagctgttt
     1441 tatcagtatc aatggcattt gcttcgggaa caacagttgg caatggcggg gatgaaatcc
     1501 gttttacatt cgaagatgcg cgcatcgaag ctattcgaat tatcaacaac atcaaagaat
     1561 gctcattcga ctctacgact tctgctgacg ttagaagttg gattttgaat aatagtatgg
     1621 cacttgcttc tgatatccaa aacagtgccc atcaatggac taatgaagac aggccgacgt
     1681 gcgcatggac acaattatct gctcgatcag aaatagtctt atcttacccc agctgtgccc
     1741 tgaatatgtc cacccagaaa gccgcgcagc ttcttattca tgaatccgtt catcatttcg
     1801 gaatcaacaa cgagtctttt gctgatgccg tggccgtggc actcaatcaa gcgaccccag
     1861 gtgttatttg cagcaaaagc acattgccag aaaataccaa gtggaaagtg cgtgatcggt
     1921 tgaggcagat gcttcctcca aactcaaaaa cagacagcat ttactttggg tcatacaaag
     1981 atactgggtc ttattgcaca gtattgctaa gtacaggaga agttacggat ccaaacaagg
     2041 tcaaaataga cagttttaca atcagtgtgc ttgaggggcg gtttaatgga tccggggttg
     2101 gtatcagtat tagtgtgctg gccaacacag aaggatcacc gtatttgcct ggatatgtaa
     2161 cattttataa agatgatggc gacctgctat ccatggatta tataacgaag tacttaaatc
     2221 aaaaatggga agcttcctct cttacagtaa actcgataaa cagcagcatt actgaagtta
     2281 caattgcaaa caaaaatgcg ccaagaaatc cgatcattaa aacctgcatt ctcgagaatt
     2341 gatttgttat ggaggtccca ttgaagaaaa tgtttgtgtt ggctctcgct acgttgactg
     2401 gggtaaatgc gtttggagcg actggtaagt ccgcgaagtt agatactgaa aaaaaatata
     2461 ttctggtcga tgttactggc tgacaatgga ttaactgata cctactacaa ggggagcgcc
     2521 atgaagatta atggcgacca agactcttcg gcgtcagtcc accttcccta ataattacgc
     2581 ctaataatag gaactatgga ttgagatctg tagcggcagc agccggacga cttgaaggag
     2641 acgtctctga tacttcttgg tcaaattcaa ggctgtcgga gttcaggtat tttccgttca
     2701 cttcaaaaaa gtagttcttc tcaccgccgg caagctgttc aacttgtcga catggccgaa
     2761 attcgtagtc aggggcccaa gcctggagtt cactctcctt ggataaaaac tctccaaccc
     2821 tgcgtaatag ctcgggttca gaaaggtttt gattgtcggt ataagtgaac tcgtataatt
     2881 tcatttattt aatccttacg ctggatttta tagcactttt gaggccaaga gccaaggtaa
     2941 gtttgggggg cggttatgaa caggcaggag ctattagaaa aaagagggga cctgtcgcca
     3001 ttcctgattc atcttacccg tagcggagat ctgaagctgg ataaagacat tttctcgctc
     3061 ccgcaggata gagttgtcca gatcaccgca aaagagagcc ttggatcaat cattggatca
     3121 cgaagaattg gtgcgcgatc ggcgttcggt tatttcaatt acaaggttcc acttaaacgg
     3181 gccgacggac gggtactcaa ttccacctct catgttcgac gtgactggct ccgcagcgtt
     3241 tgctttaccg agacgccgct cgatcacgtt tatttgcaga tgcagactat cgttggccgt
     3301 cagcttcatt tcgaatcgta tgggttggcg ttccgagaat cagtagtccg cggagccaat
     3361 ggtaatccgg tcctttactt ccaaacaaca aatcaaaata ttcgatcagg cttcgatcga
     3421 ttggcagtag atccgttggc tcatcatttt aaatcgatga tgccgttggt tgaagggttt
     3481 ggtccgccgt ggttcccacg gtatgggggc cccgccgaga ttgattttcg ttgggaacgt
     3541 gaatggagaa taactggcga ttttagcttt accttgtctg atgttgcctt tggtttttgc
     3601 cctgcggatg aaatcgctca atttgaaact ttggttggcg gcacgtttcc gttcgtggac
     3661 ccgcttggaa atctggaagc aaataaaaca aaacttcgag gctgggccca tttgcgcgat
     3721 ctcaagtgac tagcgctgac ctccaatctg ggaatacagc agatccggcg aaccccattt
     3781 gctcgctttg gactgctcca atagcttcac ctgggcgtgt cggatttggt tggtgatgcg
     3841 aggtaactgg ccggtcattg cggcggccag agtcagcgtg agtgcgagtt taatgacgtt
     3901 atcgagtccc atggcgttta ctccttttgg gtttggattt gttgatcgtt ttactttcag
     3961 aaagattggc agattcccaa ggctggtagg tcacaacatt gccatcacgc ttcagaatca
     4021 cctcagggtg gaagcgccgg tttaaaaagg ggccattttc accggtttac cggtcccata
     4081 tgggccacca taaccggttt aaaaatggac actttagcat tgcataaagg gtgacctcgg
     4141 ccatcggcat ttttgccgag ccgatgccac cccgattttt tttcgagtga ttaggctttt
     4201 tcttttttag ccttcaactt tgcttgacgc tgat
//
LOCUS       DATBTE010000072        18897 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_417298, whole genome shotgun sequence.
ACCESSION   DATBTE010000072 DATBTE010000000
VERSION     DATBTE010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 18897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18897
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417298"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VIG33_02735"
     CDS             complement(<1..392)
                     /locus_tag="VIG33_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269777.1"
                     /translation="MKWVSCYLVAVLFSWGALAYTPDQYAQQVVQKDLPVSLRWFGKA
                     AQSERLSQVEIYDLYQSALPATQVNKQLGISIKGSIERMLILLDGARYFEQVQKASEE
                     FQNKKELVSKTLQIANDEMNRFQYGEDP"
     gene            993..1973
                     /locus_tag="VIG33_02740"
     CDS             993..1973
                     /locus_tag="VIG33_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEY8269778.1"
                     /translation="MQQPPAPQNPPAPLDLSKVSNQEILFRLERLSRSERKITHLILC
                     HINEVELRKLYSELGYDSLYKYLTQHLHYGGSAAYDRMQAARVLKISPEIAPKIEEGS
                     LNLSQLVKVEQSLKQEQKSGKALSPQSTQELLGKLENKSGSETEKILASELNQTPKTQ
                     QKLKPQKDESVRLEFTLTQEQYQILKKAQHLISHSVPENNLAKAITYLAQAYIKKVEG
                     SKKNKAKTEGSSLTPSRTEMPFKVYKTLRQKRKFISLKIRREVFAKAQHCCEFVNPLT
                     GKRCASQFQLQLDHIQPLSCGGSDEITNLRALCGVCNWREAQRWGLERPL"
     gene            complement(2210..3031)
                     /locus_tag="VIG33_02745"
     CDS             complement(2210..3031)
                     /locus_tag="VIG33_02745"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8269779.1"
                     /translation="MARFFLCWSFLLGLSACSSLLYFPSQGQYYNPAKIPLAYDDVFI
                     TNKEGQKIHAWYFHSQGPSKGTFLFFHGNAENLTSHFIGLYWLPAQGYSYLIFDYPGY
                     DKSDGSPTPESTVTAGKEVLRWLAQHKGDDPLYIYGQSLGGNIALRVTLDMKNEIPIK
                     AVIIDGSFLSYKRAGRAVLAKNWITWIMQPLTYLVLSDKYAPRKIEDLPPIPLLVIHG
                     ENDPVVPVSQGKDLYARAKEPKQLWLLPKGSHGDSFFTEDGAYRQKLLDYLNSLK"
     gene            3363..5288
                     /locus_tag="VIG33_02750"
     CDS             3363..5288
                     /locus_tag="VIG33_02750"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEY8269780.1"
                     /translation="MKTVIALLFIFFSLSPLHLVRAETSAYTQALQKAHELGLAQEPQ
                     WLLLGHYKKNLLNQWKSDISDPNFFISPEGATNPSAELDSLLASLWSPANEKDMDQDP
                     RCRFPARLSWLKKNLRLEFPEEEQDPLLQRCRMYLRYRSVIKAHSLSFVFSSYYANSP
                     GSAFGHTFFRINKKPGPDGSRSELLDHGVGYAAQVTVTNPMLYAFFGLAGVFKGVFTN
                     LPYYYKVREYNDFESRDLWSYDLNLTPEEVDMVARHLWEVGNSYFDYYFLTQNCAYHM
                     LTVLEAAAPRYRLSARVPYYVIPSDTIKAITAEPGLVKEVHFRPALRSVFTQRYDRLS
                     TRDQAIFRAYMKDLDLSILLAEPEMNRVILLDTALDYMDMIYPENLVNPLSEGAKKKN
                     TLLQARASIAVTSKRFEAKLPENERPDLGHNSARAAIGFGRNYLGNSATTLEFRFALH
                     DLLDSHTGYPENSQLEFGHLRGQVDRDLQQARLDDFDIFRVTAMNPLSLFSNKLSWRV
                     HVGVHRFKEGACEKDCLTGGLGFGAGYAHYLDHEENLLLYGLLESEAYYDVHFKDQPY
                     KVAVGPTLGLLAKIGDQLKWSVEDTYLFQTVRAQSDDHKFSTELRYLAKHDWNLGLRY
                     NHEIKSDESFLMVYRFF"
     gene            5642..6154
                     /locus_tag="VIG33_02755"
     CDS             5642..6154
                     /locus_tag="VIG33_02755"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY8269781.1"
                     /translation="MESNFAVSWEVLREGHWWVLLTAVFSHNLFFHLFINMFVLNSFG
                     NAVEPVLGSKYFLIFYLIAGIFSSFTHALVSAFLLGQPDLPAVGASGAIAGVVLLFAL
                     FFPREKLLFFGILPLPAIWGALIFVGLDVWGLISQAEGGGLPIGHGAHLGGAFCGIVA
                     YLYWRKRLRV"
     gene            6238..6414
                     /locus_tag="VIG33_02760"
     CDS             6238..6414
                     /locus_tag="VIG33_02760"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HEY8269782.1"
                     /translation="MDEEIGSEKGTLFETLVLHEIKTHSDYFQKRWQVHYWGTPSENE
                     VDFIISKGKVSMRG"
     gene            complement(6581..7399)
                     /locus_tag="VIG33_02765"
     CDS             complement(6581..7399)
                     /locus_tag="VIG33_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8269783.1"
                     /translation="MKEFKSQKIKSVIKELLKKKKITYDELAEQMECSVPTIKRILGP
                     EELTLNRLLQLCEIVEIDLADLEELLKETEIKEERFTPAQEEFLAKNQSFFAYLMKLF
                     SGETPKQIAESCHLTQRSTDKYLIGLEKQGLIRVTGKQRVKPAFKNIPALGSGLLGKV
                     YYQNFIRNTTQFFIDTITEGYRKKFRDEKTGEQMSTKFGVHVSKITRASYQMWIEEQE
                     RVRKNFEKLAAFEEKTKDPSELMTVVICQGCTLVRNDDKGLNLLESAMGEVKNL"
     gene            complement(7650..9227)
                     /gene="murJ"
                     /locus_tag="VIG33_02770"
     CDS             complement(7650..9227)
                     /gene="murJ"
                     /locus_tag="VIG33_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY8269784.1"
                     /translation="MVPGSSSKEVSKKGVHHALNMAFGTFSSRILGLLRDMALAALFS
                     RTITDAWTAAFRLPNLFRRLLGEGSLSVSFIPVFVEARMDSPLRAQNLVNGFYTLLLV
                     FLALVTAVGIFFAEPLMRLLLDASYGAEKFAITVRMAQIMFGFIFFVATYAYFMGILN
                     ALGKFALPALAPTLFNISMIISTLTPGAVFSFEGDGLAWGVLAGGALQMGILVPTLSK
                     LGAMPRFQWRGFNEDIRRVLRNMVPGFVGMGLLQFTTIVNLKYASGLAEGAISSIYWA
                     DRLLELPLSLVSVSLGTALLPTLSMLWGQRNHKQMSETTNYYLRLNLFIAIPAALGLY
                     FLARPIVEVLFLRGRFTQVDAETTATVLKVYAGLLISASCARVFVPAYYAIKNTWFPA
                     VASGVSLLMHIGLAPLFMKSSGLEGLVLSSFLSATLNSVLLLSFYPKLVGAFDYFKLL
                     VHIMKFILAGAGLVVCLQVYEPLVAWFEGGVLLQALALALTILSGAVVYGVLSFVLKA
                     EEMKALSRPKSDIKITK"
     gene            9277..9546
                     /gene="rpsT"
                     /locus_tag="VIG33_02775"
     CDS             9277..9546
                     /gene="rpsT"
                     /locus_tag="VIG33_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY8269785.1"
                     /translation="MANHKSAAKRARQSTRRTAVNSKRKGTVRTEEKKLVKALEAKNV
                     TALPTLLSALTSKLTRAAQKGVFSKNTASRKIGRLSARVHALLTK"
     gene            complement(9548..9910)
                     /locus_tag="VIG33_02780"
     CDS             complement(9548..9910)
                     /locus_tag="VIG33_02780"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY8269786.1"
                     /translation="MSDFEDLSSIRRKFPRRTLVKKVGVLFGGEYLICQSGEIGEGGM
                     SIISEYALTEGRELVLSFQIPKGTFVTLRGMIRSAKPSQGVVIHGIAFDVIPLGFKRQ
                     IRAFVSARISTINSSQRI"
     gene            complement(9971..11674)
                     /locus_tag="VIG33_02785"
     CDS             complement(9971..11674)
                     /locus_tag="VIG33_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269787.1"
                     /translation="MRIFLILFCLFLIHTSSLWRPSQTLAGGSGDTLAHAAMPGFYCK
                     NIFPKYLSAQYLETRKLPSDQFLMGDAELSTSFDSPFPMILTCPLSSLGSSVQFHIFA
                     MLEIFLVLLSAFLVGRVMKLSEPWLLAYVLFVWWTGFYAARINHHFTLLPSIWGFQFI
                     LWAGLTIRWRELHSVLLRAFIMGLVFAGTFHNTAMLSLPLLAILTLRLWQDRAQQKSL
                     AWVKTLGLATVIFLGTFVFFFAPAIISYFQSGQPHSVVERTHFSLALLHAFLPLWAGG
                     MALPIEATNPLDLVILGIVLVSLLHKKFWQEPLRRVLILLAAFCFVWSLGPELRMGSS
                     LPGEGSLENYFFTLLERIPPLSVSRAPGRFMVVTALSFIYLAFDYLRLFWMERAGNFK
                     VALALIAWILVTGPVLTHEIFVPTWDYKSNIPQKAVEVIRASSVKTIVAHIPTAMAAD
                     PLQNFFQLFHQKRISAGYLAYTAYTSETMKTLLEDPFLSHTNCFGDALVFKRTSFLEN
                     FEVLHEHLKKTHIGFLVLNKNILNDAACSDLVNWLRELVKQPWVQVLDETPVLLVFDV
                     R"
     gene            complement(11699..12718)
                     /gene="holA"
                     /locus_tag="VIG33_02790"
     CDS             complement(11699..12718)
                     /gene="holA"
                     /locus_tag="VIG33_02790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY8269788.1"
                     /translation="MALIDAQKFYRDLEKGEFSPLYFLFGEEAYLLNQCVERFKYAVL
                     TDGAIDFNYSLFYASDADVSNVRDAVETLPMMAPKRLVILKEAQELSDREWAELDPLI
                     TTPVDSTVFVILASRVDKRKKPIRLMLETAECVEFKKPYDNQIPGWVKYIAGTLGLDI
                     AEDAILLLAKLVGSHLSEIESELKKLADFMGERKRIEVGDVAQVVSRSKEENVFDFTR
                     AIGESDRVRALEHLVHLLDQGQSEIGIVSLLARHIRLLLSVKRGLEHGLTGAKLAHSA
                     QVPPYFLDNYVMQSRLWTVKKLEYTLIILAETDKALKSSPLSSHIWLENLVLKTCSQS
                     QQAIV"
     gene            complement(12831..13391)
                     /gene="lptE"
                     /locus_tag="VIG33_02795"
     CDS             complement(12831..13391)
                     /gene="lptE"
                     /locus_tag="VIG33_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY8269789.1"
                     /translation="MLGSLLLQVLLGGCAYRIGMSSRSLPGGYKYLSIPVFKNVTQEI
                     GIEVGFTNALIQEFERSQVGRVTSENLSDVKIVGVVEAIQYLPGTKRTAGESSAPYLP
                     EGTVLASDYRILVSVKVSIVRQSDNTQIWTGGFTGERTYNAPLVTLAGVNSVNPLYNL
                     SARRQNIDSVANDMMAEAHDRITENF"
     gene            13474..14643
                     /locus_tag="VIG33_02800"
     CDS             13474..14643
                     /locus_tag="VIG33_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEY8269790.1"
                     /translation="MNSPDNLFDYSLLAPGPVSLHPEVRKALALPMIHHRTPEFDKIL
                     ARVLTGLKKIFQTSQNVYMHSSTGSGGMESLLVNVLSPGDKVIVIVSGKFGERWAQMA
                     EVFGAQVIIHNVPWGEAVKTADIKALLEQHPETRALLCQACETSTGVVHPIRELALLT
                     ENRMNTLFLVDAITALGAFPLPMDEWKIDGIVAGSQKALMLPTGLSFVAFSKKAESFF
                     EKATCPRYYFDIRREKKANASGETFFSSSVSLVRALDVVLNLIEQQGLDPLFASIHRR
                     AEATRIFAPKLGFGLYAKAPSDSVTALTVPAGVDGQKIRLHLEQKYNITVMGGQDQAK
                     GKIIRVGHMGYIQDAEMLRLINCLGLSLQHFSANSLSTQKIQDTVSELKIWLETH"
     gene            14643..15632
                     /locus_tag="VIG33_02805"
     CDS             14643..15632
                     /locus_tag="VIG33_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEY8269791.1"
                     /translation="MKKKILITDRFAQDVYLYLQQQSQFEVLRTDSPTHLPLEHIVNA
                     HALIIRSRTPINEELLKKARNLQVIVTATSGFDHIDLEATQKWGVTIMHTPSANIESA
                     AQLTLALVLSCVTHLQQAHKMIKAGDWNRENLVGIELSGRTYGIVGLGRIGTRVAQLA
                     QAFGMNVVAYDPYQEDEHFEKYKIPRFSYEEVLKTADCLSFHVPKTLETDCMLNRSHF
                     EYIHRGITLINTSRGSVIDELDLCEALEKGFIRAVGLDVFEKEPLPRTSKLLTYQNVV
                     LTPHIGATTEDAFYKASNIAAQKLVEFFLDGSTSDALPPQVPWYGATPFKSDN"
     gene            15702..17000
                     /locus_tag="VIG33_02810"
     CDS             15702..17000
                     /locus_tag="VIG33_02810"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEY8269792.1"
                     /translation="MSGTVVVGAQWGDEGKGKLVDVFAANADFVVRYQGGSNAGHTIV
                     VNGVKTILHLVPSGILHPETTCIITSGVVVDVFGILQEIRKLKDNGHLQNPKQLLISD
                     SATAILPYHKALDEAREKALEDDKIGTTGRGIGPAYEDRASRRAILIGDLFDRASLRK
                     KLELGLSEKNFLMEKFYKGQTFDIDELMKQLEGVANELLPYRCRDTSSIIAKALKNGK
                     RVLFEGAQGTMLDLLHGTYPYVTSSSTVAGSACIGAGIGPIHIQKVIGVFKAYTTRVG
                     SGPFPTELNDEIGKKIQMEGNEFGATTGRSRRCGWLDLVALKYAIRVNGITNLALMKL
                     DILSGHERLGICTGYMLNGEAISELPPNSYELEKVEPVIEYLPGWKEDISKLTTLKDL
                     PRATMNFIDYIVTQLGTPVDVISIGPGRDQTLWVKPLFTK"
     gene            17189..17264
                     /locus_tag="VIG33_02815"
     tRNA            17189..17264
                     /locus_tag="VIG33_02815"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17222..17224,aa:Lys,seq:ttt)
     gene            17290..17365
                     /locus_tag="VIG33_02820"
     tRNA            17290..17365
                     /locus_tag="VIG33_02820"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17324..17326,aa:Glu,seq:ttc)
     gene            complement(17431..17844)
                     /locus_tag="VIG33_02825"
     CDS             complement(17431..17844)
                     /locus_tag="VIG33_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269793.1"
                     /translation="METQRVMREEKEEIEEIIKITVTKEAGENLTEILAMVNEGFGAG
                     KVNRQDIASWIIDRFKKSFSDSDIQQIRKDFFSEKVLLESILKQAKASGDVPDFLREA
                     LKKYLHLKLDDTRSMLETLNEKRDVLRPNFKRSQG"
     gene            complement(18014..18535)
                     /locus_tag="VIG33_02830"
     CDS             complement(18014..18535)
                     /locus_tag="VIG33_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269794.1"
                     /translation="MAAQKKGLIDDNWLSEKVLRQSTPQDVTQVFGSELYKLPDLVFD
                     IRLPGQPFRVAFEIERTRKAQMRYDGLVNAYAAASGVHLVLIAFNDRSVFELIRGAAR
                     RLHYPQERRPIAFCKISELHNSWTNFPMMIGDQALGFEQYIENLKSLLQTTQKSVSDI
                     ESEKESLFAVGAG"
BASE COUNT         5294 a         4521 c         4006 g         5076 t
ORIGIN      
        1 tgcgggtcct caccgtattg gaagcgattc atctcatcat tcgctatttg cagggtttta
       61 ctcacaagtt cttttttgtt ttgaaactcc tcagaagctt tttgtacctg ctcaaaataa
      121 cgagccccat cgagcaaaat cagcatccgc tctatggagc ccttgataga aatccccaat
      181 tgcttattca cttgagtcgc gggaagcgca gactgataaa ggtcatagat ttcgacttga
      241 ctaagacgtt cagattgagc ggctttgcca aaccaacgaa gactaaccgg aagatctttt
      301 tgcacaactt gttgagcata ttgatccgga gtgtacgcca gagcccccca cgaaaacagc
      361 acagcaacca agtaacaact cacccatttc atgcagggct tatagcacaa aatgcggatt
      421 ctcgctcttc ctctaacagc aaatgaaaca tcttctgaag tgtctagttt ctagacagtt
      481 ataatgttct ttcttctcgg ttttacaaaa acttgcccac agagttggca caggaaatag
      541 cggccacgaa gggcgaaacg agcaaaacaa gaattctcta gaattccctc aaagcctcga
      601 gctaaccacc agcccatacc caagcgaagt ctctcgagaa aatcacaatc acgagactat
      661 aaagctcccg acaagacggc atttccggga agccttgcgt aaaaataaat atttaaaaaa
      721 tatctcttgc gtggattttc tcactctctc agagggcgaa agcttggttt cttattctct
      781 gccagagggc ttctcatctc acaaaattac tctctataaa acgagtctaa tgaatcccct
      841 aaacgctatc acatagactt tccccatgta cttctctctg aaataaatct caaaactcac
      901 ttgaaaaaac tcgtggtata ccttctctca tcttcttttt acgaaagaaa ttttttaaaa
      961 tttatttaac ttattaaaaa ttggagtgcc tgatgcaaca accccctgcc cctcaaaatc
     1021 ctccagcgcc cctggatctt tccaaggttt ctaatcaaga aatccttttt cgtttagaaa
     1081 ggctttcacg ttcggaaaga aaaataaccc acctcatcct ttgccatatc aacgaagtcg
     1141 agctgagaaa actttactca gagctcggct atgactctct ttataagtat ctcactcaac
     1201 atttgcacta tggcggaagt gccgcctatg atcggatgca agccgcgcga gtgctaaaga
     1261 tcagccctga gatcgcccct aaaattgaag agggttcgtt aaatctttct caattagtca
     1321 aagtggagca aagtcttaaa caagaacaaa aatcagggaa agctctttcc ccccaaagca
     1381 ctcaagaact tctaggaaag ttagaaaata aaagtggctc tgaaacagag aaaattctag
     1441 cttctgagct caaccaaacc cccaagaccc agcagaaact caaaccgcaa aaagatgaaa
     1501 gtgtgagatt ggaattcacg ctcacgcaag agcagtatca aatcctaaaa aaagctcagc
     1561 atctgatttc ccattcagtg ccagaaaaca atctagccaa agcgatcact tatttagctc
     1621 aagcttatat taaaaaagtg gagggctcta aaaagaacaa ggctaaaacc gaaggctcca
     1681 gcttaacgcc gagccgcacg gaaatgccgt tcaaggttta taaaactctc cgccaaaaac
     1741 gaaagttcat ttcccttaaa atcagaagag aggtctttgc aaaagcccag cactgctgcg
     1801 agttcgtgaa tcccctcacg ggaaaaagat gtgcttccca gttccaactc cagctggacc
     1861 atatccagcc tctgtcctgc ggaggctcgg atgaaatcac caaccttcgg gccctttgcg
     1921 gcgtgtgcaa ctggagggaa gcccagcgat ggggacttga acggcctttg taagtaaatg
     1981 aaacattctg caaagtaaaa tagttttgga gtagagctct tggagtaagt tgaaatgcga
     2041 cgttagacgt ttactctgcc accgggaccc tgaaccaaca atcggagctt ttttattaag
     2101 gttcccactt gacggctctc tgttctttta tttctcctat tctagacgaa ctcgatcata
     2161 gaagggaacc ggggtttgag gcgaggcaga caatagtaaa aattcagatc tatttcaaac
     2221 tgttcaaata atctagaagc ttttggcgat aagctccgtc ttcggtaaaa aaggaatctc
     2281 catgagaacc ttttggcaat agccagagct gtttgggctc tttggccctg gcgtacaaat
     2341 ccttgccttg gcttacggga acgacaggat cattttctcc atggataacc agcaagggaa
     2401 tgggaggaag gtcttctatt tttcttggag catatttgtc actcaatact aaatatgtaa
     2461 gaggttgcat aatccacgta atccaatttt ttgcaaggac ggcccgaccc gcccttttat
     2521 aggataaaaa acttccatca ataatcaccg ctttgatggg aatttcattt ttcatatcta
     2581 aagtcaccct gagtgcgata ttgccgccca aactttgccc gtagatatac aaagggtcat
     2641 cccctttgtg ttgtgctagc cagcgcagca cttctttacc ggctgtcacg gtggattccg
     2701 gagtaggact cccgtcggat ttatcgtagc cagggtaatc aaatatcaaa taactgtaac
     2761 cctgtgccgg tagccaatag agaccgatga agtgagaggt cagattttcc gcgttgccat
     2821 gaaaaaacag aaaggtccct ttgctaggac cttggctgtg aaaataccat gcatgaattt
     2881 tttgcccctc tttattggta ataaacacat catcataagc aagaggaatt ttggctggat
     2941 tataatattg tccttggctt ggaaaataca agagggaaga gcatgcgcta agtccgagca
     3001 agaagctcca acacagaaaa aatcgagcca ttacttcgct ttatgacagc agaacacatg
     3061 ccatcctttt atgttttttg atattcatca acaatgaaga ataagcacat tctacttccg
     3121 caacaaataa aaataatgac agggaatgtt atcaatagaa ctctttctaa ccaattgttt
     3181 ggaatttctt tcaaaacctt gaattgtact gatggccccg taaatgaagt tacagatagc
     3241 attatttctg tgatcatgaa tgattcacag gttgctactc aatgcaactt gggttaattc
     3301 gatagttcat taaaatttac taactgcgct gtttgcccag cgcagttttt ttatggcctc
     3361 aaatgaaaac tgtaattgcc cttttattta tctttttttc gctttccccg cttcacctgg
     3421 ttcgagcaga aacttcagcc tatactcaag cactgcaaaa agcccatgaa ttgggtttgg
     3481 ctcaagaacc gcagtggctc cttctcggcc attataaaaa aaatctcttg aaccaatgga
     3541 aaagtgatat ttcggatccg aactttttta tcagtccgga aggagctaca aatccgagtg
     3601 ctgagctgga cagtctttta gcctcccttt ggagtcctgc gaatgaaaag gatatggatc
     3661 aagatcctcg ctgtcgtttt ccagccagac tctcttggct taagaaaaat ctgcggttgg
     3721 aatttccgga agaagagcag gatccccttc ttcagcgctg ccggatgtat ctacgctatc
     3781 gttccgtcat aaaagcgcac tcattaagtt ttgtattttc ctcttactat gccaatagcc
     3841 ccggttccgc ttttggccat actttttttc gtattaataa aaagcctggt cccgatgggt
     3901 cccgcagcga actcttagat cacggagtgg ggtatgccgc ccaggtcact gtgaccaatc
     3961 ccatgcttta tgcatttttt ggtttagctg gcgtctttaa aggcgttttt acaaacctgc
     4021 cttactacta taaggtccga gaatacaatg acttcgagtc tcgagatctt tggtcctatg
     4081 atttaaatct tacaccggaa gaagtggata tggtggctcg acatctgtgg gaagtcggca
     4141 attcttactt cgattattat tttttaactc agaattgcgc ttaccatatg ttaaccgttt
     4201 tagaggcggc agccccgcgc tatcgcttat cggcaagggt gccctattat gtaattccct
     4261 cagacacgat caaggccatc acggcagagc ctggcttagt aaaagaggtc cactttcgcc
     4321 cagcactacg cagtgtattt actcagcgat atgacagact gagcacccgc gaccaagcga
     4381 tttttcgcgc ttacatgaaa gacctggact tatccattct cttggctgaa ccagaaatga
     4441 atcgagtgat tcttttagat acggcattag attatatgga tatgatttat ccagagaact
     4501 tagtgaatcc gctctccgaa ggagccaaga aaaagaacac cctattacag gcccgggctt
     4561 ccattgcggt cactagcaaa cgcttcgaag cgaagctccc tgaaaacgaa agacccgacc
     4621 tcgggcataa ctctgcccgc gccgccatcg gctttggcag aaactatctg ggaaattccg
     4681 cgaccacact ggagtttcgt tttgctttgc atgatctttt agattcccac actggttatc
     4741 ctgagaattc tcaattagaa tttggccacc ttcgcggaca ggtcgaccgc gacctccagc
     4801 aggcccgctt agacgatttt gatatttttc gtgtcaccgc catgaatccc ttaagtttat
     4861 tttcaaataa gctgtcctgg cgcgtccacg ttggagtcca tcgttttaag gaaggcgcct
     4921 gtgaaaaaga ttgtctcaca ggaggtttgg gtttcggcgc cggttacgcc cattaccttg
     4981 accacgaaga aaatctcctc ctttatggat tgctcgaaag tgaagcctac tacgacgttc
     5041 actttaaaga tcaaccttac aaagtggcgg tcggccccac cctgggtctg cttgcaaaaa
     5101 ttggcgatca gttaaaatgg tccgttgaag acacttatct ttttcaaact gtccgagccc
     5161 aaagcgacga tcacaaattt tcaacggaac tccgctatct cgctaaacac gattggaacc
     5221 tcggtcttcg ctataatcat gaaataaaga gcgatgaaag ttttttgatg gtgtatcgat
     5281 ttttttaaat ttttttgttt tctggcatgt gacagaaaat tccactcatc ggtcttgatt
     5341 caggttctca aatttggaat cattgagatc tgatatgcgt tcatcagtaa agccggggtc
     5401 atctggatac tttgaatccg cgctttaaag cagttagaaa gaggggcgta tcgctatgaa
     5461 atctttgatt tcatgagcga gcaccccgct cttttttctt cctaatagtt ctttgctttc
     5521 cttcttggac cctctatatt taaaaataat gaaatcaggg cgtctcattc ctataattat
     5581 tcttctcaac agtgtcgttt tctttctatg gtcctacggc ggaacggaaa atcaggagtt
     5641 catggaaagt aatttcgctg tgagctggga agtccttcgt gagggtcatt ggtgggtttt
     5701 gttaaccgcc gtcttttctc acaatttatt tttccatcta tttattaata tgttcgttct
     5761 caatagtttc ggcaatgctg tggaaccagt tttaggttca aaatactttc tgatttttta
     5821 tttgattgcc ggcatcttta gctcttttac tcatgccctg gtttcagctt ttctgcttgg
     5881 acagcctgat ctgcccgctg tcggcgcctc tggagccatt gctggagtgg ttctgctctt
     5941 tgccttattc ttcccccgag agaaattatt attttttgga atcctccctc ttccagcaat
     6001 ctggggcgcc cttatttttg tagggctgga tgtgtggggc ctgatcagcc aagctgaagg
     6061 cggtggcctt cctatcggcc atggcgccca tctggggggc gctttctgtg gcatcgtcgc
     6121 ttatttatat tggcgcaagc gtcttcgagt ttaagaaatc actctttaat tccatgtttg
     6181 agcatggctt tgcgcagttc gttccaggac tctccgtttt gtagaagggt tttaaagttg
     6241 gatgaggaaa taggttcgga aaaaggcact ttgttcgaaa ctctggtttt gcacgaaatt
     6301 aaaacccact ctgactattt tcaaaaaagg tggcaagttc attattgggg aactccaagt
     6361 gagaatgaag tcgatttcat catttccaaa ggaaaagttt caatgcgggg ctagaaacct
     6421 tgattgatgc tggcaaaatt aaggccgcct atggagttta tttaggttcc gaacagctca
     6481 aaagcggtcc agtgacagtg ctgccatttc gaaattttat ttcgctgatg tacgccggaa
     6541 agcttatctg accattaagg gcaaatgagt aaaaaaaccg ttaaagattc tttacctctc
     6601 ccatagcgct ttccagaaga tttaaacctt tatcgtcgtt cctaaccaaa gtacacccct
     6661 ggcaaataac cacggtcatt aattccgagg gatcttttgt tttctcttca aaggcggcca
     6721 gtttttcaaa gtttttccgg acccgctctt gctcctcaat ccacatctga taagaagcgc
     6781 gagttatctt tgaaacatgc accccaaatt ttgtagacat ttgctccccg gtcttttcat
     6841 cacggaattt cttgcgatac ccctcggtga tggtatcgat gaaaaactgc gtcgtatttc
     6901 taataaaatt ctgatagtaa actttgccca gcagaccact acctaaagct ggaatattct
     6961 taaaggcagg cttcaccctt tgttttcccg tcacccgaat caacccttgc ttctccaagc
     7021 caatcaaata tttatcagta gatctttgag ttaagtggca ggattcagcg atctgcttcg
     7081 gagtctcccc actgaaaagc ttcatcaggt aagcgaaaaa gctttggttc ttggccagaa
     7141 attcctcttg cgcgggagtg aagcgttctt ctttgatttc agtctcttta aggagttcct
     7201 caaggtcagc cagatcaatt tcaacgatct cacacaattg taacaagcga ttgagggtca
     7261 gctcctccgg ccctaggatt ctttttatcg tcggtaccga gcactccatt tgctccgcga
     7321 gttcgtcata ggtgatcttt ttcttcttta agagctcctt gatcacactt ttgatttttt
     7381 gagatttaaa ttccttcatc gttttaacct ctgtaacttc tgcgaaccgt aggaactgag
     7441 aattcttttt ctaagatata ggtttcaaat tggctgttat taataagtat taattttctt
     7501 aagatcgttt ttaagcattt cattttgaac ttccttcttt atttcagaaa ggcaccgctt
     7561 tcttgaaaac aaagatgccg gagtttcaag gcctcaacaa ggtcaaaact agaggtatca
     7621 caaaatgatc cccacagcga attatcagct cattttgtga tttttatatc actttttggc
     7681 ctacttaagg ccttcatctc ctcggccttt aagacaaaac tgagaacacc ataaaccacc
     7741 gcacccgata aaatggtgag agctaaggcc aaagcttgca gtaaaacgcc gccctcaaac
     7801 caagcgacaa gcggttcata gacttgcaaa cagaccacca agcccgcgcc cgccaaaatg
     7861 aacttcatga tatgcacaag cagtttaaag taatcaaaag cacccacaag ttttggatag
     7921 aaactgagaa gcagaacact gttcaaagtc gcactgagaa aggaagacag caccaacccc
     7981 tcaagcccgg agcttttcat aaacaaagga gccaagccaa tatgcatcaa caaagaaact
     8041 ccagaagcca cggctgggaa ccaggtattt ttaatcgcat agtaagccgg cacaaaaacc
     8101 cgcgcacaac ttgcggaaat taaaagtccg gcatagacct tcaataccgt cgccgtggtt
     8161 tccgcatcta cctgggtaaa tctgccgcgc agaaaaagaa cttcaacaat aggccgagct
     8221 aagaaataca aacccaaagc cgcagggatg gcgataaaca gattcagtcg caaataataa
     8281 tttgtggtct ctgacatttg cttatgattt cgctgtcccc aaagcatgga caaagtgggc
     8341 aataaagcag tgccgagact cactgacacc aaagaaaggg gcagttccag aagacgatcc
     8401 gcccaataaa tagaagagat ggctccttcg gccaaaccgc tggcatattt taaattcaca
     8461 atcgtggtga attgtaaaag ccccattccc acaaaccccg gcaccatgtt gcgcaacact
     8521 cggcgaatat cttcattgaa accccgccac tgaaaacgcg gcatggcccc aagtttggat
     8581 agagttggca ccagaatccc catctggagc gctccacccg ccagcacccc ccacgccagg
     8641 ccatcgcctt caaaagaaaa cacggcccca ggggtcagag tcgaaataat catggaaata
     8701 ttaaaaagcg tcggtgccag agctggcagg gcgaattttc caagggcatt taaaatcccc
     8761 ataaagtagg cataggtcgc cacaaaaaag ataaaaccaa acataatttg ggccatacga
     8821 acggtgattg caaacttctc ggcaccataa ctggcatcca aaaggagcct catcagcggc
     8881 tcggcaaaga aaattcccac cgccgttaca agggctaaaa aaactaataa gagagtgtaa
     8941 aacccattga ccagattctg ggcccgtaag ggagagtcca ttcgggcctc gacaaagact
     9001 ggaataaagc tgaccgacag agagccctca cccagtaacc tcctgaaaag attaggaagt
     9061 cgaaaggccg cggtccaagc atcggtgatg gtgcgactaa aaagtgcagc caaggccata
     9121 tcccgtaaaa gtcccagaat tcggctcgaa aaagttccaa aggccatatt cagcgcatga
     9181 tgaactcctt ttttcgatac ttctttgctt gaactgcccg gaaccatatg ttatcacctg
     9241 tgttcgttta aaaaagctta gtgtggaggt tatcccttgg caaatcataa gtctgcagca
     9301 aaaagagccc gtcaatctac tcgtagaact gctgttaaca gcaaacgtaa gggcactgtg
     9361 cgtacagagg aaaagaaact cgttaaagct cttgaagcta agaacgtaac agcgcttcca
     9421 actttgttga gtgctttaac tagcaaattg actcgtgcag ctcaaaaagg cgttttcagc
     9481 aagaacacag cctctcgcaa aatcggccgt ctttctgctc gcgtccatgc tcttttaaca
     9541 aaatagttta tattctttgc gacgaattta tagttgaaat ccgagctgat acaaaagctc
     9601 ggatttgtcg tttaaaaccc aatggaatga catcaaaagc aattccatga atcaccacac
     9661 cttggctggg ttttgcagat ctaatcattc cccgtaaggt cacaaaggtt cccttgggga
     9721 tttgaaaact caaaacaagt tctcgcccct cggtcaaggc gtattcgctg ataatggaca
     9781 ttcctccttc accaatttcc cctgattgac aaatcaagta ttcaccccca aaaaggactc
     9841 ctactttttt caccaatgtt cggcgcggaa acttgcgtcg gatagaactc aaatcttcga
     9901 aatcactcat agtgtgtata tcggattttt aatctgaaaa catgagactg gatcaatatc
     9961 cagcgcccca ttagcgcaca tcaaaaacca acaggactgg agtctcgtca agcacctgca
    10021 cccagggttg tttcaccaat tcgcgaagcc agttcacgag atcggaacag gcggcatcat
    10081 ttaaaatatt tttatttaaa accagaaaac caatatgagt ttttttgaga tgttcatgaa
    10141 gaacttcaaa gttttcaaga aaagatgtcc ttttaaagac taaagcatcg ccgaaacaat
    10201 ttgtatgact caaaaaagga tcttccaaca aagtcttcat cgtctcagag gtgtaggcgg
    10261 tgtaagccaa gtagccagca ctgattcttt tttgatggaa aagttggaaa aaattctgca
    10321 gcggatcggc agccatagcc gttggaatgt gcgccacaat agttttcaca gaactcgctc
    10381 gaatcacttc cacagctttt tgtggaatgt tggatttata atcccaggtc ggaacgaaaa
    10441 tttcatgggt caaaaccggg cctgtgacta aaatccatgc tatcaaagcc agggccactt
    10501 taaaatttcc tgctctttcc atccagaaaa gtctcagata atcaaaagcc aagtaaatga
    10561 acgacaaggc tgtcaccacc ataaatcgac ccggcgcccg ggagactgat aaaggtggaa
    10621 tacgctcaag caaagtgaaa aagtaatttt caagagaccc ctcacccggc agtgaacttc
    10681 ccatccgcag ctctggcccc aatgaccaaa caaaacaaaa agcagccagc aggattaaca
    10741 ctctacgcaa aggctcttgc caaaactttt tatgaagcaa agatacgagg acgattccca
    10801 agatcaccaa atccagcgga ttcgtcgctt caatgggcag ggccatgccg ccagcccaca
    10861 agggtaagaa tgcatgcaat agagccaagc tgaagtgagt tcgttccacc actgaatggg
    10921 gctgaccact ttggaaataa cttataatcg ctggagcgaa aaagaaaacg aaggttccaa
    10981 ggaaaatcac cgttgccagc cccaacgtct tgacccaagc aaggctcttt tgttgagccc
    11041 gatcttgcca aaggcgcaga gtcagaattg cgagcagcgg caaactcagc attgcggtat
    11101 tatgaaaggt tcccgcaaag accagcccca ttataaaggc acggagtaaa acagaatgga
    11161 gttcacgcca acgaattgtt aaaccggccc acaaaataaa ttgaaaaccc caaatgcttg
    11221 gcagcaaggt gaaatgatga ttgattcttg ccgcataaaa tccggtccac cacacgaaga
    11281 gaacataggc taaaagccaa ggttctgaaa gtttcataac cctgccgacc aagaaggccg
    11341 agagaaggac cagaaaaatt tctagcattg cgaagatgtg aaactgcaca gaagacccga
    11401 gtgaactcaa aggacaggtc agaatcatcg gaaaaggact atcaaaactg gtcgaaagct
    11461 ccgcatcccc catcaaaaac tgatctgagg gcaactttcg ggtttctaaa tattgagcag
    11521 atagatattt cggaaatata tttttacaat agaatcccgg catcgccgca tgagccaagg
    11581 tatcccctga acctcccgcc aaagtttgac tgggccgcca tagagaggag gtatggatta
    11641 agaacaaaca aaagaggata agaaagattc tcatgccacc cccttttcct gggatttatt
    11701 acacaattgc ctgctgagac tggctgcacg ttttcagcac cagattctca agccaaatat
    11761 gagaagataa aggagacgat ttcaaagcct tatccgtctc cgccaggata attaacgtgt
    11821 actccaattt tttgacggtc cacaaccgac tctgcatcac ataattatct aagaagtaag
    11881 ggggcacttg agcagagtga gccagctttg cccccgtgag gccgtgctca aggcctcgtt
    11941 ttacggaaag caataagcgg atatggcgag ctaacaaaga gacaattcca atttcacttt
    12001 gcccctgatc caagagatga accagatgct ccaacgcgcg cacgcgatca ctttcgccaa
    12061 tcgcccgcgt aaaatcgaaa acattttcct ctttagaacg agaaaccact tgagccacat
    12121 caccgacttc aatgcgtttt ctttcgccca taaaatccgc gagttttttc agctcggatt
    12181 cgatctcaga aagatgactg ccgaccagtt tcgctaaaag taaaatagca tcctcagcga
    12241 tatctaatcc caaagttccc gctatatatt tcacccaacc tggaatttga ttgtcgtagg
    12301 gttttttaaa ctccacacac tcagcggttt ccagcatcaa acggatgggt tttttgcgtt
    12361 tatccacacg ggaagcgaga ataacaaaga cagtgctatc cactggagtg gtgatcaaag
    12421 gatctaactc ggcccactca cgatccgaga gttcttgggc ttcttttaaa attaccaagc
    12481 gcttgggcgc catcatcggc aaggtttcaa cggcatcacg cacgttcgaa acatcggcat
    12541 cagaagcata aaacaaagaa tagttaaaat cgatggcacc atccgtgagg acagcatatt
    12601 tgaagcgttc tacgcactgg ttcagtaaat aggcttcctc accaaagaga aaatacaacg
    12661 gagaaaactc gcccttttcc aagtcacgat aaaacttttg agcatcgatc aaagccactc
    12721 aatattcccc gtagattgcc cttaactaat cttgtaataa tctcgtaata atctcgtaaa
    12781 taacctgtaa tatactcagg ctctccgtga tgctgttaac caaaaaaagt ttagaagttt
    12841 tcagtaatac gatcatgagc ttcggccatc atgtcgttag caacggagtc aatgttctgc
    12901 ctgcgtgctg aaagattgta aagtggattc acggaattga caccggctag cgtgactagg
    12961 ggcgcattgt aagtgcgttc tcccgtaaaa ccacctgtcc aaatttgtgt attatctgat
    13021 tgacgaacga tacttacttt cacggaaaca agaatacgat aatctgaagc cagcaccgtt
    13081 ccttctggaa ggtagggagc tgaagattcc cccgccgtcc gttttgttcc aggaagatat
    13141 tgaatggcct ccaccactcc cacaattttt acatccgaaa gattttcaga ggtgactctt
    13201 cccacctggg agcgctcaaa ctcctggatt aatgcgttgg tgaatcccac ttcaatgccg
    13261 atttcctggg tgacattttt aaacaccgga atggataaat atttatagcc tccaggaaga
    13321 ctgcggcttg acatgccaat tcgataggca caaccaccga gcaaaacctg gagtaaaagc
    13381 gacccgagca tcaaaaatct tgaagttttc accggattag ttgagtatag cagagtcaaa
    13441 ctttcaagaa agaaatgact gaaagagcta aaaatgaact ctcctgacaa tctttttgat
    13501 tatagccttc ttgcgccagg tccagtgagt ttacatcccg aagtccgtaa ggctttggct
    13561 ttgccgatga ttcatcatcg caccccagaa ttcgataaaa ttctagcccg agttcttacc
    13621 ggtctaaaaa agatttttca gacctcgcaa aatgtttata tgcattcctc cactggcagc
    13681 gggggcatgg aatctctttt agtgaatgtt ttaagccctg gcgataaagt gattgtgatt
    13741 gtttccggaa agtttggcga gcgttgggcc cagatggccg aagttttcgg tgcccaagtc
    13801 atcattcaca acgtgccctg gggagaagct gtcaaaacag ccgacatcaa agcccttttg
    13861 gaacagcatc cagagactcg ggccctcctt tgccaggcct gtgaaaccag tactggggtg
    13921 gttcatccaa ttcgtgagct cgccctgctc actgaaaata gaatgaatac tttattttta
    13981 gtggacgcca tcaccgctct gggagctttt cccctgccga tggatgaatg gaaaatcgat
    14041 ggcatcgtgg cgggctcgca aaaagccctg atgctgccca caggactcag ttttgtggcc
    14101 ttttccaaaa aagccgaaag tttttttgag aaagccactt gtccaagata ttattttgat
    14161 attcgtcgcg agaaaaaagc caatgccagt ggcgaaactt tctttagttc ttccgtgagc
    14221 ttggtcaggg ctcttgatgt ggttttgaat ctcattgaac agcaaggctt agatcctctt
    14281 tttgccagca ttcatcgtcg cgcggaagcc acgcggattt tcgctccgaa attaggcttc
    14341 ggtctttatg caaaagcgcc gagtgattca gtgacagcgc tgactgttcc ggcgggagtg
    14401 gatggacaaa aaattcgtct gcatcttgaa cagaaataca atatcacagt tatgggtggc
    14461 caagatcagg ccaagggtaa aatcattcgt gtaggccata tgggctacat tcaagatgca
    14521 gaaatgcttc gtttgataaa ttgtttgggt ttaagcctgc agcatttctc ggcaaactcc
    14581 ctctcgacgc aaaaaatcca agacacggtc tctgaactta aaatttggct ggaaactcac
    14641 tgatgaagaa aaaaattctt attacggatc gcttcgcgca ggatgtgtat ctttatttgc
    14701 aacagcagtc acaatttgag gtcctaagga cggactcgcc aacgcatttg cctttagagc
    14761 atattgtgaa tgctcacgct ttgattatcc gcagtcgcac tcctatcaat gaagaactgt
    14821 taaaaaaagc gcgcaacttg caagtcattg tcactgccac cagcggcttt gatcatatcg
    14881 accttgaggc gacgcaaaag tggggcgtca ccatcatgca tacgccttcc gcgaacatag
    14941 aaagtgccgc gcagttgact ctggcattgg tcctttcttg tgtcactcat ctgcaacagg
    15001 cccacaaaat gatcaaggct ggagactgga atcgcgaaaa cttggtgggc attgaactca
    15061 gcggtcgcac ctacggaatt gttggtttgg gtcgcattgg aactcgagtg gcacagttgg
    15121 cccaagcctt tggcatgaat gtcgtggctt atgaccctta ccaagaagat gagcactttg
    15181 aaaaatataa aattccccgc ttcagttatg aggaagtctt aaaaacggcg gattgtttaa
    15241 gcttccatgt acctaaaact ttggaaacag actgcatgct taatcgttcc cactttgaat
    15301 atattcatcg tggaattacg ttgatcaata cctctcgggg ttcggtgatt gatgagttgg
    15361 atctctgcga ggccctcgaa aaaggcttta ttcgcgcggt gggtctggat gtttttgaaa
    15421 aagaacccct gcctcgaacc tcaaaacttt taacttatca aaatgttgtt ttaacgccac
    15481 acattggagc cactacagaa gatgcttttt ataaagcctc taatatcgct gcccaaaaac
    15541 tagtcgagtt tttcttggac ggctccactt cagatgcttt accaccccaa gttccctggt
    15601 atggcgcaac accctttaaa tctgataatt agagttcttg ccttgtcacc agcgatgggc
    15661 gaaccttttt tctaaattca ttaaatatta agaggtatca agtgtctgga acagtcgtgg
    15721 taggtgctca atggggcgat gagggcaaag gaaaactcgt cgacgttttt gctgccaatg
    15781 cagattttgt ggttcgttac caaggtggct ccaatgcggg tcacaccatt gtcgttaacg
    15841 gtgtgaaaac tattttgcat ttggtgccct ctggaattct ccatcctgag acgacctgca
    15901 taatcacctc gggtgtggtg gtggatgttt ttggaatctt gcaagagatt cgaaaactca
    15961 aagacaatgg tcatttgcaa aatccaaaac agcttttaat ttctgattct gcgacggcca
    16021 ttttgcctta ccataaagcc ctcgatgagg cacgcgagaa ggccctggaa gacgacaaaa
    16081 tcggcaccac cggtcgtggc attggcccgg cttatgaaga ccgtgcttct cgtcgggcga
    16141 ttctgatcgg cgatcttttt gacagagctt ctttgcgaaa aaaattagaa ctcggcttga
    16201 gcgaaaaaaa ctttttaatg gagaagtttt ataaaggcca gactttcgac attgatgagc
    16261 taatgaagca actggaaggt gtcgccaacg aactgctgcc gtaccgctgc cgcgatacct
    16321 cttcaatcat cgccaaggct ctgaagaacg gcaaacgcgt tttattcgaa ggagcccaag
    16381 gaaccatgtt ggatcttctg catggaactt atccttacgt gaccagctct tcgactgtgg
    16441 cgggctctgc ttgtatcggc gccggcattg gcccgatcca cattcaaaaa gtaatcggcg
    16501 tttttaaagc ttacaccacc cgcgtcggtt ctggcccttt cccgacagag cttaatgacg
    16561 agatcggcaa aaaaatccag atggaaggca acgagttcgg tgcgaccacg ggacgctccc
    16621 gtcgttgtgg ctggttggat cttgtggcgt taaaatacgc gattcgtgtg aatggcatca
    16681 ccaacctcgc gttgatgaag ctagacattc tctcaggcca cgaacgcttg ggcatctgca
    16741 caggatatat gctcaatggc gaggccatca gcgagctgcc gccgaactct tacgagctcg
    16801 agaaggtaga gcctgtgatc gagtatcttc ctggctggaa ggaagacatc tcgaagttga
    16861 caaccttaaa agatttgccc agagcaacga tgaatttcat cgattacatc gtcactcaat
    16921 taggaactcc ggtggatgtg atttctatcg gccctggtcg tgatcaaact ttgtgggtga
    16981 agcctctttt caccaagtag aaatttaaaa tgcccaacaa gtaagcgctc ttagctgact
    17041 cttaacttac tcttagctta gatgttgggc atttctttct ctttcattca acttctagaa
    17101 tttttcgtaa ttatttttat ttttcttcaa taatcgggtt gacttaaaga gcactttcta
    17161 cgagattagc aatccttgca acacacgtgg ttcgctagct cagctggtag agcacttcac
    17221 ttttaatgaa ggggtcgatg gttcgaatcc atcgcgagcc accatttttt cttttttagt
    17281 tccgggtacg ttcccatcgt ctaggggcct aggacacctc cctttcacgg aggatacagg
    17341 ggttcgaatc cccttgggaa cgccaaataa tccgcatact tagcaaaacc acctctttta
    17401 caagggtcgt tttgtatcca ctggtatcca tcacccctga gatcgcttaa aattcggccg
    17461 caaaacatct cgtttttcat tcagcgtttc cagcatagat cgcgtgtcat cgagctttaa
    17521 atgcagatat tttttcagcg cctctctgag gaaatccggc acatcacccg aagccttggc
    17581 ttgctttaag atcgactcaa gcagaacctt ctcgctgaaa aagtctttgc gaatttgttg
    17641 aatgtcgcta tcgctgaagg actttttaaa tctatcaatg atccaactcg cgatatcttg
    17701 acgattcact ttgcctgccc caaatccctc attcaccatc gccaatatct ccgtcaaatt
    17761 ttccccagct tcctttgtca ccgtgatttt tataatttcc tcgatctctt ctttctcttc
    17821 cctcatcact ctttgtgtct ccaatgtatt ctcctttcat ttttgttttt ttctgtatct
    17881 cctgctccca accccttgcc tgtttcgttg ccactttcca tcaacatcgt tgagggggag
    17941 tggcgacgaa ccgcaggaag gcaaggggtt gggagcagga ccggcctttc ggttgtaaat
    18001 ctagctacta agctcacccc gcccccaccg caaagagcga ctctttctcg gactcgatgt
    18061 ccgacacgct tttttgagtt gtttgtagaa ggctttttag attctcgata tactgctcaa
    18121 accctaaagc ctgatcgcca atcatcatgg ggaaattcgt ccaactatta tgtaactcag
    18181 aaatcttgca aaaggcgatg ggtcggcgtt cttgcgggta atgaaggcgc cttgctgccc
    18241 cacggatcaa ttcaaagacg gatcggtcgt tgaaggctat cagtaccaag tgaacgccgc
    18301 ttgcggcagc gtaggcgttt accaggccat cgtatctcat ctgggccttg cgcgttcttt
    18361 caatttcaaa ggcaacccgg aaaggctggc ccggaagacg gatatcaaaa accaaatctg
    18421 gcagtttata aagttccgag ccaaaaactt gagtcacatc ttgaggtgta gattgtctta
    18481 agaccttctc acttagccaa ttgtcatcaa tgagtccctt cttttgggcg gccaaggcaa
    18541 aatgaattgc attgtcatca tgttcaaaat gcagcgggtg cgcactgctt acaggctgaa
    18601 tgccagcatt ggctagaatt cgctttcccc gcttagagag tctaaaaacg tcttcaccga
    18661 taaagtatct cttataggct tcaaggtagc ctggaagcgc cggtttaaaa aggggccatt
    18721 ttcaccggtt taccggtccc atgtggtcca ccatagccgg tttaaaaatg gacactttag
    18781 cattgcataa agggtgacct cggccatcgg catttttgcc gagccgatgc caccccgatt
    18841 ttttttcgag tgattaggct ttttcttttt tagccttcaa ctttgcttga cgctgat
//
LOCUS       DATBTE010000073         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_417414, whole genome shotgun sequence.
ACCESSION   DATBTE010000073 DATBTE010000000
VERSION     DATBTE010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417414"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /gene="xseA"
                     /locus_tag="VIG33_02835"
     CDS             <1..1181
                     /gene="xseA"
                     /locus_tag="VIG33_02835"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY8269795.1"
                     /translation="LKDAKSQISAVMFRGHNSRLKFKITDGMEVIVRGRISVYEPRGN
                     YQILCEMMEPVGAGALQKAFEQLKAQLKGEGLFEAARKKVIPHMPRHVAVVTSPTGAA
                     IRDILNILSRRAPWLPVTIVPTVVQGETAATQICEALLKAQKLPEVDVIIVGRGGGSI
                     EDMWCFNDEKLARLIAASPIPVISAVGHEIDFTIADFVADLRAPTPSAAAELVAKSSQ
                     ELTNKLQQLQKLLNLNTQKYFKVFEQKLQMLTKGLVDPQKKLQDLILRNDDLVERLQM
                     ASDRLLSERRYKLQILDERIGSPQDLIQQKAKELEYLSQCMGKGLALGLERKKSVLGR
                     WMSVLESLSPLKVVERGYSIVKKENEVIKSTDQVKAGDLLRITLAQGLLEARVENVIK
                     "
     gene            1196..1420
                     /locus_tag="VIG33_02840"
     CDS             1196..1420
                     /locus_tag="VIG33_02840"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY8269796.1"
                     /translation="MDFEKKLSRLEEIVNKMEKGDLALEESLRFFEEGVKLSRECHQK
                     LNDAESKVKLLVSMDQNGKATTTHFSADEA"
     gene            1439..2293
                     /locus_tag="VIG33_02845"
     CDS             1439..2293
                     /locus_tag="VIG33_02845"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEY8269797.1"
                     /translation="MIQFEEELKKRIKVVDDYIHHYFSHLDLPPGLPVLELKKSMYYS
                     AGSGGKRFRPVLSLLIAELFSVPVSRILPFATAVELIHTYSLIHDDLPCMDNDDFRRG
                     KLTNHKVYGETIALLAGDALLTEAFLLIAKEYKDNGFLAAQLCEHLSRAAGVRGMVGG
                     QAIDLKAKNLTVEELTHLHNLKTGRMIQVAVEGAALVSGASHSEIENLAMFGEYLGLA
                     FQIADDILDHDEKDQEERSFVGLLGIEATKKYLHDVSEKALKALHKVSHRSAILEHMI
                     EFNRQRQK"
     gene            2290..3060
                     /locus_tag="VIG33_02850"
     CDS             2290..3060
                     /locus_tag="VIG33_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEY8269798.1"
                     /translation="MRLDLYLVQKQLAFSRSHAQELIRANQVYQIKNDKKIILKKSSQ
                     LVDEAQDQIFIEAGPANRFVSRGGLKLEGALKHCQLSVKGWNVLDVGISTGGFSDCLL
                     QAEAGSVLGVDVGHGQLHPSLLAKKPYLQHIEGLNAREIDQDPRVKGVMPKEGFDLIV
                     MDVSFISITLIIPQLVNLLKPEAFLLSLVKPQFEVGPEGLGKGGLVKDPRLYSVIEDK
                     VKKLCESSGMSVKDYFNSPIEGKDGNREFFIFAQKKKA"
BASE COUNT          908 a          656 c          792 g          913 t
ORIGIN      
        1 ctttgaaaga tgccaaatct caaataagtg cggtgatgtt tcgcggacat aattcacgtt
       61 taaaatttaa aattacagat gggatggaag tcatcgttcg tgggcgaatt tctgtttacg
      121 aacctcgtgg caattatcaa atactttgcg aaatgatgga acccgtaggt gctggagctt
      181 tacaaaaggc ttttgagcag cttaaggctc agttgaaagg cgagggtctt tttgaggccg
      241 cccgtaaaaa agtcattcca cacatgcctc gccatgtggc agtggtcacc tctcccaccg
      301 gggctgcgat tcgggatatt ttgaacattc tttcccgtcg tgccccctgg cttcccgtga
      361 ctattgttcc caccgtagtt caaggtgaga ccgcagctac gcaaatttgc gaagccctct
      421 taaaagctca aaagcttcct gaggtagatg tgattatcgt aggtcgcggt ggcggctcta
      481 tcgaagacat gtggtgcttt aatgacgaga agctcgcgcg cttgattgcg gcctctccca
      541 tccctgtcat ttccgctgtc ggccacgaga tagattttac gattgcggat tttgtggcag
      601 atcttcgcgc tcccacgcct tcggcggcgg cggagcttgt agcgaagagt tctcaggaac
      661 tcactaataa acttcagcag ctacaaaagc ttttgaatct taatactcag aagtatttta
      721 aagttttcga gcagaaattg cagatgctga ctaaggggct cgttgatccg cagaagaaac
      781 tgcaagatct tattttgcgg aatgatgatt tagtggaacg cttgcaaatg gccagtgatc
      841 gtctgctttc tgagcgtcgt tataaattgc aaattcttga tgagcgtatc ggctcacctc
      901 aggatcttat tcagcaaaaa gccaaagagt tggagtatct ttctcagtgt atgggaaaag
      961 gtttggcctt gggcttagag cgaaagaagt ctgttcttgg acggtggatg tctgttttgg
     1021 aaagtttgag cccattgaaa gtggtggaaa gaggctattc cattgttaag aaagaaaatg
     1081 aagtgattaa aagcacggat caagtaaaag ccggtgatct tttgcgtatt accttagcac
     1141 aaggattgct tgaggcccgt gtagaaaatg ttattaaata agtagggagc atcccatgga
     1201 ttttgaaaaa aaattaagtc gccttgaaga gatcgtgaat aaaatggaaa agggagactt
     1261 ggccttagag gaatctttga ggttttttga agagggcgta aaactctctc gcgagtgtca
     1321 tcaaaagttg aacgatgccg agtctaaagt gaaattactg gtgagtatgg atcaaaacgg
     1381 caaagccact acgactcatt tttccgctga cgaagcttaa ggattcttct tggacctcat
     1441 gattcaattt gaagaagagc ttaagaaaag aattaaagtc gtggatgact atattcatca
     1501 ctatttttct catttagatt taccaccagg tctgcccgta ctagagctga agaaatcaat
     1561 gtattactcc gctggaagcg gtggaaaaag atttcgccca gttttatcac ttttgatcgc
     1621 agagcttttt tcagttcctg tttcccgcat cttgcccttt gcaacagcgg ttgaacttat
     1681 tcatacgtat tctctgattc acgatgatct accctgtatg gataacgatg atttccgccg
     1741 tggtaaattg accaaccata aagtttatgg ggaaactatc gcattgcttg cgggggatgc
     1801 cctattgacc gaagcgttct tgttaatcgc caaagaatat aaagacaatg gcttcttagc
     1861 ggctcaatta tgtgagcatt tgtctcgtgc cgcaggagtg cgtggcatgg tgggtggcca
     1921 ggctattgac ttgaaggcaa aaaatctgac ggtcgaagaa ttgactcatc ttcataattt
     1981 aaaaacgggc cggatgattc aagtggccgt agaaggtgct gcccttgttt caggagcaag
     2041 ccattctgaa attgagaatc tggctatgtt tggtgaatat ttggggcttg ctttccaaat
     2101 cgctgatgac attttagatc atgacgaaaa agaccaagaa gagcgcagtt tcgtgggcct
     2161 tctggggata gaagcgacga agaagtattt gcacgatgta agcgaaaagg ctctcaaagc
     2221 actgcataaa gtttctcacc gttcagcgat tctggaacat atgattgaat tcaatcgcca
     2281 acgccaaaaa tgagactgga tctttatttg gttcaaaaac aattggcttt ttcacgttcc
     2341 catgcgcagg agctgatccg agccaatcaa gtttatcaga taaaaaacga caaaaaaatc
     2401 attttaaaaa aatccagcca attagtggat gaagctcaag atcagatctt tatagaagca
     2461 gggcccgcga accgttttgt ttcgcgaggg ggcttgaagc ttgagggtgc tttgaagcac
     2521 tgtcagctct ctgtgaaagg gtggaatgtc ttggacgtgg gaatttccac aggaggcttc
     2581 tcagattgcc tacttcaagc ggaagcgggt tcagttctag gggtcgatgt gggccatgga
     2641 caactccatc cttctctatt ggcgaaaaaa ccttacttgc agcatattga gggcctcaat
     2701 gcccgggaaa tcgatcaaga tcctcgagtc aagggggtga tgccaaaaga gggcttcgat
     2761 cttattgtta tggatgtttc ctttatttcg atcactttaa ttattccgca acttgttaat
     2821 ctactgaagc ctgaggcttt cttattgagc cttgtaaagc cgcagtttga ggtgggccca
     2881 gagggtcttg gaaaaggagg attggtgaaa gatcctagac tctattctgt gattgaagat
     2941 aaagtgaaga agctttgcga aagctctggg atgagcgtaa aggattactt taattcacca
     3001 atcgagggaa aagatgggaa tcgcgaattt ttcatttttg ctcaaaagaa aaaagcataa
     3061 aaagaatcag caagaaaatc agaaaagaaa ctattacagg ttcttaatct taaacctttt
     3121 tttaataact gcttgccaaa atctaccttc ggcgtcagcg ccggtgccgc ctcctaaata
     3181 tgaagcgcaa actctcccgc ctcaggtatc agctcctgaa gtgccggttt ctgcctcttc
     3241 tccaatccca gccattccta aaattggct
//
LOCUS       DATBTE010000074         4073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_485694, whole genome shotgun sequence.
ACCESSION   DATBTE010000074 DATBTE010000000
VERSION     DATBTE010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485694"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VIG33_02855"
     CDS             complement(<1..446)
                     /locus_tag="VIG33_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269799.1"
                     /translation="MNTPLVYKSLIGLAFVAAVVSFQLTALASNMAKSPAHASSSPTD
                     KNVLETAESSFKKGEYDKVTELLWKNIDKLNRKGLLLLAVTHEKKKEAANMIKVANML
                     TSKDSKDYEGFYLLGSAQLMSKKNSEALESLKAALEINPKYQPAYE"
     gene            466..1329
                     /locus_tag="VIG33_02860"
     CDS             466..1329
                     /locus_tag="VIG33_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269800.1"
                     /translation="MALLDLVQKSKKQSSLLSHHGQAVVEYVLMLVVSVAMVMALGYQ
                     IFKPFQAFLKSYMGDYIQCLLETGELPALGNPDSQALLEDASCNAQFQAATLSGGRPS
                     KSANSANPNQELQSKSASESSSSQGGGGGGGSGPSPSSRGGNLLVSSMKKKNATESKG
                     GLDNKVTEISLAEKSQFYNRKNSYSSDAALLAAKTTQVGIAGMTEDEKRKQERKEESR
                     RTVASGENMGPPLKKIPIKKPEVKTSGEIEDPPFTFGNFLKFLLIAAIVIALIVVIGS
                     QVLKVIKSQEK"
     gene            1476..1898
                     /locus_tag="VIG33_02865"
     CDS             1476..1898
                     /locus_tag="VIG33_02865"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEY8269801.1"
                     /translation="MKNVIEINRKKVFMRHEVSQLLPLIYRLTEESQQEVRKITNFIE
                     AYTDKKCLAVQELQGRLDEIVNNWQAKIEKLGGEPKGLWLADFDNGHGYFCWKFPETE
                     ICFWHGYQDGFMGRTLITEKSRPEIYENSNSPNQPDAW"
     gene            1858..3483
                     /locus_tag="VIG33_02870"
     CDS             1858..3483
                     /locus_tag="VIG33_02870"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY8269802.1"
                     /translation="MRIAIAQINPTLGDFEYNSEKILEYIRRAQEKECELVVFPESAL
                     FGYHPYDLLERTKLVLKQEKYLQKIHKQIPKGIGILLGAFTRNPSLRGRPYFNSAAFL
                     VKGQKVQFFNKQLLPTGDVFDEARFIQPGSMKKNYLNFKGKRFFVTICEDIWAWPDHR
                     GVSPYFKNPIKDVSKRKVDLVINLSASPFFPGKLKQREYMTKKTAQYFKAPILYANLV
                     GAQDEVIYDGGSFILDQKGKALLRCHQFEEDLNVLNLETSKAWSPAEVMQGPEALRKA
                     IVLGIRDFCRKTGLQKLHLGLSGGIDSAVAACLAVEALGAANVVGLAMPGPFSAPESL
                     KLAEGLAKNLQIKLNVASIGESYQALLKTLEESFGLKDFGITHENLQARLRGMILMAY
                     SNDKNSLLISTSNKSEYASGYSTLYGDMCGGLAPLGDLTKKQVYELAKIYNAQDAIIP
                     EAIIERPPTAELRPNQKDQDTLPPYDLLDASVVNLIEHWGSVKNATDRWLLPVILRTE
                     FKRWQAPPILKVSEHSFGRGRRWPIAHKASESI"
     gene            complement(3511..>4073)
                     /locus_tag="VIG33_02875"
     CDS             complement(3511..>4073)
                     /locus_tag="VIG33_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY8269803.1"
                     /translation="VEIGAVRFVNGEPEAIFSTLIDPLRPIPAGASKVNGIFDAMVKG
                     KPLIDSILPSFAEFCGDDMLVAHNAPFDAQFLTADIKRHETSAPKGIIIDSLPIARKV
                     FPGLPNYKLGTLIQYLKIPSADFHRAEADASYCGQLFFQMMKRISVAGQAPQVANLVA
                     LTGKAELRFPQIIRQPKQMDLFGGLL"
BASE COUNT         1166 a          807 c          937 g         1163 t
ORIGIN      
        1 ttttcgtagg caggttgata ctttggattg atttcaagag ctgcttttaa cgactccagg
       61 gcttcgctat ttttcttact cataagctgt gcgcttccaa gtaaataaaa gccctcatag
      121 tctttagaat cttttgaggt gagcatgtta gccaccttaa tcatatttgc agcttccttt
      181 ttcttttcat gtgtaacggc cagcaaaagg agtcctttgc gatttagttt gtcgatattt
      241 ttccaaagca gttctgtaac cttgtcatac tctccctttt tgaaagaaga ttccgcagtt
      301 tcgaggacat ttttatctgt tggagaggat gaggcgtggg ctgggctttt ggccatgtta
      361 ctggctaaag ctgtgagctg aaatgagaca acagctgcga caaaggccag tcctatcaag
      421 cttttgtaaa ctaggggtgt attcatatgg tatattatta cctaaatggc cctcttagac
      481 ctagtgcaga aatcaaaaaa acagtccagt ttattgagtc accatgggca ggccgtggtg
      541 gaatatgtgc tcatgctggt agtgagcgtc gctatggtta tggccctcgg atatcagata
      601 ttcaaaccat tccaagcgtt tctaaaaagc tatatgggag attatattca atgcctttta
      661 gaaacaggcg agcttccggc acttggaaat ccggactctc aagccctcct tgaagatgca
      721 agttgtaatg cgcaatttca ggcggcaacg ctttctgggg gacgaccctc aaagtctgcg
      781 aactcggcga atcctaatca agagttgcaa tccaaatcag catctgagtc ttcaagttct
      841 cagggcggtg gtggaggagg cggttcggga ccatcgccat cctcccgcgg aggaaatttg
      901 ctagttagct ctatgaagaa aaaaaatgct acggaaagta agggaggatt agataataaa
      961 gttactgaaa tctctcttgc agaaaagagc cagttttata atagaaaaaa ctcttactct
     1021 tcagatgctg ctctgcttgc agctaagacc actcaggtag gtattgctgg aatgactgag
     1081 gatgaaaaaa gaaaacaaga acgtaaagag gaaagtcgca ggacagtggc ttctggcgaa
     1141 aatatgggcc ctcctttgaa aaaaatccca attaaaaaac ctgaagtgaa aacctcaggt
     1201 gagattgaag atccgccttt tacttttggt aattttttga aatttctttt aatagcggcc
     1261 atagtaatag ccctcattgt ggtgattgga agtcaggttc tcaaagttat aaaaagtcag
     1321 gagaaatagt tcaacacact gcaagtataa ttgtttctgg cgcgttggaa atttcttcaa
     1381 atgccatctt aacttaccta taattttcta tattgcttgc aagagttttc taattgctag
     1441 atatcaatgc tcggaaattg atttgggggg cctgagtgaa gaatgtgatt gagatcaatc
     1501 gcaaaaaagt gtttatgcgt catgaagtgt ctcaactgct tccattgatt tatcgcttaa
     1561 ccgaagaatc tcagcaagaa gtccgtaaga tcactaattt tatcgaagct tataccgaca
     1621 agaaatgtct tgccgtacaa gaactccagg gccgattgga cgaaattgtc aacaattggc
     1681 aagctaagat tgaaaagctt ggcggtgagc caaaggggct ttggcttgct gactttgaca
     1741 atggacacgg gtatttttgt tggaagttcc cagaaaccga gatttgtttt tggcatggtt
     1801 accaagacgg cttcatgggg cgtaccttga ttacagaaaa aagcaggccc gaaatttatg
     1861 agaatagcaa tagcccaaat caacccgacg cttggtgatt tcgaatataa ttcagaaaaa
     1921 atcttagaat atataaggcg tgcgcaagaa aaggaatgcg aacttgtcgt ttttcctgaa
     1981 agcgcactct ttggctatca tccttatgac ctccttgaaa gaaccaaact tgttctcaaa
     2041 caagaaaagt atcttcagaa gatccataag caaattccca aaggcatcgg cattttactt
     2101 ggagctttta ctcgcaaccc ctcgctcaga ggaagacctt attttaactc cgcagcattc
     2161 ttagttaagg gtcaaaaggt tcagtttttt aataaacagc ttttgccgac gggggatgtg
     2221 tttgatgaag ctcgttttat tcagcctgga agcatgaaga aaaactatct caattttaag
     2281 ggcaaacgtt tcttcgttac catctgtgag gatatttggg cttggccaga ccaccgtggt
     2341 gtttccccct attttaaaaa tcctattaag gatgttagta aaagaaaagt cgacttggtt
     2401 atcaacttaa gcgcttctcc cttttttccg ggaaagttga agcaacgaga gtatatgact
     2461 aaaaagacag cccagtattt taaagctcct attttatatg ccaatctagt gggcgcccaa
     2521 gatgaggtta tttatgatgg tggcagcttc atattggatc agaaagggaa ggcccttctt
     2581 cgctgccatc agtttgaaga agacttaaat gttttaaatt tagaaacttc aaaagcctgg
     2641 tcacccgctg aggtgatgca gggacctgag gctctgcgta aggccatcgt attagggatt
     2701 cgtgacttct gccgtaagac aggcttacag aagcttcacc tgggattgag tggtggcatt
     2761 gactcggctg tagcagcttg ccttgctgtc gaggcgctag gagctgcgaa cgtggtcggc
     2821 ttggcgatgc ccggaccttt tagtgcaccg gaaagtttaa agcttgcaga ggggttggct
     2881 aagaacctgc agataaaact aaatgtagcg tctattggag agagttatca ggctttactc
     2941 aaaactctgg aagaatcttt tggacttaag gattttggaa taacccatga aaatcttcag
     3001 gctcggttgc gcggcatgat cttgatggca tactccaatg ataagaactc tttgcttatt
     3061 tccacgagca acaaaagtga gtatgcttct ggttactcca ctctctatgg tgacatgtgt
     3121 ggcggattag cgcctttggg tgatttgact aagaagcaag tttatgaatt agcgaaaatc
     3181 tataatgctc aagacgctat tattcctgaa gcaattattg agcgtccgcc gactgcggag
     3241 cttcggccaa atcaaaagga tcaggatact ttaccaccct atgatttgct ggatgcctca
     3301 gtggtaaatt taatagagca ttggggatct gtaaaaaatg ccacagatcg atggctgttg
     3361 cctgtgattt tgagaactga atttaaacgc tggcaagctc cgccaatctt gaaagtctca
     3421 gagcattcgt ttggcagggg tcgacgttgg ccaatcgcgc ataaagcttc tgagtctatt
     3481 tgattatttt aattttttgt agattcagag ctaaagcaag cctccgaata aatccatttg
     3541 ctttggctga cggataattt gagggaagcg aagctctgcc tttcctgtaa gcgccacaag
     3601 atttgccact tggggcgctt gacctgcaac agaaattctt ttcatcatct gaaagaatag
     3661 ctgaccacaa tatgaagcat ctgcctcggc tctatggaag tccgcactag gaatttttaa
     3721 gtattgaata agcgtgccaa gtttatagtt tggcaatccg ggaaatactt ttctagcaat
     3781 agggagactg tcgataatga ttccttttgg tgcgctagtc tcatgtcttt taatatccgc
     3841 agttaaaaac tgagcatcga aaggagcatt atgagccact aacatatcgt cgccgcaaaa
     3901 ctctgcaaag gaaggaagga ttgaatcgat caatggcttg cctttaacca tggcatcaaa
     3961 gatcccattt actttagagg cccctgctgg aataggccta aggggatcga tcaaggttga
     4021 aaaaatagcc tcaggttcgc cattcacaaa gcgaacggct ccaatttcaa caa
//
LOCUS       DATBTE010000075        10900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2398921, whole genome shotgun sequence.
ACCESSION   DATBTE010000075 DATBTE010000000
VERSION     DATBTE010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10900
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2398921"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VIG33_02880"
     CDS             <1..201
                     /locus_tag="VIG33_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269804.1"
                     /translation="MLNFTLFRGRHRPMDRKLQKGQVLIEGLLLMLFLVSVLVVLMNF
                     TKKQNEVFKKNELPTKLKYQNR"
     gene            164..649
                     /locus_tag="VIG33_02885"
     CDS             164..649
                     /locus_tag="VIG33_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269805.1"
                     /translation="MNFLQNLNIKIDKTLLIVFVIFGLVIILKSFLPTEKNDDNVKDS
                     SQESFDVDTMIPAGYMLIPLEFSNAESLASLSGQFSIVDLYATSEKGKRGFKVASAVK
                     LLRAPLNPQQFAVLVKEEDSAKIVAMEGPFFAALKNPHEAAKSSAKKSVGNPLIIHYG
                     E"
     gene            655..1527
                     /locus_tag="VIG33_02890"
     CDS             655..1527
                     /locus_tag="VIG33_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269806.1"
                     /translation="MLRKFLQPFLILASIAGGAKPASAILFQAPTASSEDFHKYALRK
                     NQQTYTQWMQENTLKNDSEAHPQVLEFSQQALKGGSSKQAMNDWNLLLRSIDINKADR
                     EVFTLLAEKLKWQTALCRYLLLDPELESILENPDLARKCLSQASSVLSTLSNVLNVGD
                     LLVVDGKVFNRHQPPTKLMTGTYQWRIISNLQEDRRFVGTAEEFLKQNFVLQNWVSGD
                     CGDYKFDHKDFSLLLQSQIYFNEACISPAMPPEKNFSTWTAEHKKLIWGLGILAAGIT
                     AFQLKDKTLVITRP"
     gene            complement(1546..2799)
                     /locus_tag="VIG33_02895"
     CDS             complement(1546..2799)
                     /locus_tag="VIG33_02895"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8269807.1"
                     /translation="MFFKVSHLYDSHVHWLGTGQIAVGLQLYDLASAEDIAKIKVEPW
                     YFRGQWLVGFGWDHNKWPDKKLPSKEILDQCFPHFPAMLQRADGHCSWLNSMALTQLG
                     FEANHPGILQEEEHFKAYSSLPKLSDMQIRQSLKQGCAIFNQAGFTHIRDMTCDEQQW
                     RAALDLELAGELTLYVQENFLCEHPKDIDEVLALLEKAKSQETPHLKAQGIKIFFDGT
                     LGSDTAWLSKCNCVNYKFSWTAEDLQTLLKKTWSKGFEVAVHTIGDEAAHQVVLAARQ
                     VMATNQFTGWLNLEHVEVLRPETVQKLKALHVRCHMQPCHWLSDRVWLKEKLGDLYKY
                     AFPWGTLSRSGIPLYFGSDSPVARSSFWDNQKALTESARAKIPALQGEIAEFHSWRGE
                     TIGETYTVFEDQKLHELVFDGKNIL"
     gene            complement(2854..4617)
                     /locus_tag="VIG33_02900"
     CDS             complement(2854..4617)
                     /locus_tag="VIG33_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269808.1"
                     /translation="MKFGTRLAALAVSTLTLPSLSANAQMAWGTGQAMGQQACPYNAQ
                     AGEGASSYLEDYNEIKRAKADEEAKLREMKRKLRTLEKSIDKARDAITNVVDGRYSDS
                     ILSHMDNSRLCGDYDTDKIPKTRSKVRGSDDGEELPRAESGGETAQANLTPLSGFSPQ
                     QWAQVCIQGVKGGIDGRVCSTAPYSSSDRGRYTPNECKRGLASYRKDTLDYQKTQDDI
                     AKSEERIERYKDELPETLREAKARYKEDRQAGTEGDICLTCMARGSGYQVESQGPDWA
                     GVAANVGVGLMATYMGYQTNKMVARYNSDLGWPTQAYPAWGYGLPFLANGLYGALGGG
                     VGGGAFGCAGAVGGGGFPYGTMGGMGPFGASGGLYGNVGMNGPFGYPNGMFGNPWGGG
                     MYNGGMGPWGLNGGFGGGGYPGMMGGGFGGGFGFGYPQMGGGFPGMGGGFPGMGGGYP
                     GMGGGYPGMGGYPGMMDGGLGSMQMQQAMMQMQQQQQQLYMQHYQQQMQNQMARQQTL
                     MGLNVELQNLMYRIQQVQMGGASSGYLGGGLGGQVSIGGGISIGGGFNGGGNGVLPAP
                     GVSGSTNTTIPNGVVVPAGSR"
     gene            4819..5922
                     /locus_tag="VIG33_02905"
     CDS             4819..5922
                     /locus_tag="VIG33_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEY8269809.1"
                     /translation="MAKHLILCVDDELDNVDALERLFRKHYTVLKATSGAQGLAILGE
                     NPDVALIISDQRMPNMTGVEFLEKAQLSHPKALRILLTGYTDIESVIQSVNQGQIYRY
                     LTKPWDSTDLLNTVDHAIQKYEVSKELEKKNTELAAALHELKSLDKAKSNFMILINHE
                     LKTPLTSILSFIGLLNETQMDDEQKLFVNRISRAADKLRSIIDDVLLIVHAETNQLKV
                     HLSPVSFSGVEKLLNPEVSNVLMYKHQSLLEKNMGSSVSADRELIRQVLNRLIHNAAK
                     FGYDNTEITIEATKKNEKIIYSVFNKGPRIESSVIDKILRPFFIDEDVMNHSTGMGLG
                     LNICQSILNLHQSKLKIENTDEGVCVSFNLPAV"
     gene            6041..7336
                     /locus_tag="VIG33_02910"
     CDS             6041..7336
                     /locus_tag="VIG33_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEY8269810.1"
                     /translation="MRFLFIIFLSIQAHAEPLLLKIGDDHKLPLPANHRVWIQNRKIL
                     SVATRGGFLVLNGNGEGFTTLQVGTKSYQVQVIQPLKKTLLQNFEKELHHILGLRLII
                     KKYQVTVSGKLYRWEDWLQLAKISEETGVTYAMSAEIPASLQAKALSHWQQEFSVAGL
                     PPVPIHFAHPLQARLAIDPQQFAKYEAILGNFGVLLEKDSQALDIAPVVKVQITVAEV
                     RRDFALNYGLQWPSGYSATILANGQKEFENLMLNATAFEHQGRGKILASPNLLCRSGK
                     EAEFLAGGEFPIKMKTFKMQDVVWKKYGILLKVKPKADSSGRMSIAIETEVSTIDNSR
                     SVDGIPGLLTNRISSHFDLAHSQTIVLSGLIKNEEGKSTEGLPLLSRIPILGALFSSR
                     DFKENRTELVILVRPTIVKENATEPPSDSPAHLRDLKYE"
     gene            7329..8432
                     /locus_tag="VIG33_02915"
     CDS             7329..8432
                     /locus_tag="VIG33_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY8269811.1"
                     /translation="MNNNLQNIFNQTQERLRKISDRGYGHEDSWRTQNAEKFKQSLEE
                     ILKPASEETRRRILAEYFSCGPLEDILQDSEITEILVNGPASIWFEKQGHLQRHPDFF
                     LSEASYTNCLHRLCEQAHCQSTVEHPATTGHFKEFRLTLVRQELTHNHHHLSLRRHPK
                     SPWTLERLQEQNWAGEEDIKKLRKLIEKKKSFLIVGSTGTGKTSLINALLQTIPTNER
                     AIVIEDIPEIELPNSACMKMITREDPQGLLKTIDQSELLRHSLRLRPDRIVMGEIRGA
                     EAKDLLMALATGHDGSFGTLHANDPRQALIRLEMLIQMGAPQWNLQAVRRLIHLSLDC
                     ILMVEKTPEGNRKFQGAYKICSLEENGFLIEKV"
     gene            complement(8429..8821)
                     /locus_tag="VIG33_02920"
     CDS             complement(8429..8821)
                     /locus_tag="VIG33_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269812.1"
                     /translation="MEKISRILPPSRRVVATDVESSQPVRPGAPDYGRPTQKPLNIQD
                     RVSLSSLANGRSAEPSTYKNFKEGARARVVDELAEKFFNPRTVAKEGEGPRSEETLKR
                     MEEDQSYFPVTPAKMRNLAEEERPLSPI"
     gene            8965..9258
                     /locus_tag="VIG33_02925"
     CDS             8965..9258
                     /locus_tag="VIG33_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269813.1"
                     /translation="MLLYQITDIFDISKIKYALVGGYALALHGLVRATMDVDLVLNLK
                     QSDFEQAETALGKIDLQSRLPIRAQDIIKMRKEYIENRNLIAWSFVDYKNPTR"
     gene            complement(9605..10315)
                     /locus_tag="VIG33_02930"
     CDS             complement(9605..10315)
                     /locus_tag="VIG33_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8269814.1"
                     /translation="MKKIAIVTGASRGLGLGISEALAQRGYKVIMAMRTPDKAQKQVN
                     ELKMKDLDVVPMSLDTSQEKSINDFAEQIRREYGFVDVLVNNAGIMADSEDGGSSSVF
                     KTKASTIQKTFTTNTLGPFLLIQKIFPLMKQEGYGRIVNVSSGMSQLSQPQTAYAGYR
                     ISKTALNMVTNIFASEAQGSDICVNAVCPGWVRTDMGGPNAERSIEQGIKGIIWAATL
                     PKGGPNGGLFRDGEPIPW"
     gene            10636..>10900
                     /locus_tag="VIG33_02935"
     CDS             10636..>10900
                     /locus_tag="VIG33_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269815.1"
                     /translation="MCLKVSDPSIYYLLLLLILGLIHTFPLWNLRENLAGGYGDPMAH
                     AAIGGWYCGNVLTGNFNSTQFMAPSGADLSGNYDSPFPFVLTCP"
BASE COUNT         3017 a         2570 c         2334 g         2979 t
ORIGIN      
        1 atgctcaact tcactttatt tagaggaaga caccgaccaa tggatcgcaa gcttcaaaaa
       61 gggcaagtcc ttattgaagg gctccttctg atgctctttc ttgtcagtgt tttagtggta
      121 ctgatgaatt ttactaaaaa acaaaatgag gtctttaaaa aaaatgaact tcctacaaaa
      181 cttaaatatc aaaatagata agactctctt aattgtcttt gtgatttttg gccttgtaat
      241 tattttgaaa agttttctgc ccacagaaaa gaacgatgat aatgtcaaag acagttctca
      301 agagagtttt gatgtcgata ccatgattcc tgctgggtat atgctcattc ctttagagtt
      361 tagcaacgct gaatccctgg cttcactctc aggccaattt tcaatcgtgg atctgtacgc
      421 tactagcgaa aaaggcaaac gaggttttaa ggtggcttcc gcggtgaaac tgctgcgggc
      481 gcccctcaat cctcaacaat tcgcagtgct tgtcaaagaa gaagacagcg caaagattgt
      541 ggccatggaa ggccctttct ttgctgctct caaaaatccc cacgaagcag cgaagtcttc
      601 ggcgaaaaaa tcagttggga atcctctcat tatccattat ggagaataga actcatgttg
      661 cgaaaatttc ttcagccatt tttaattctg gcctcaatag caggtggagc aaaacccgca
      721 agcgctattt tatttcaagc ccctacagcg agttctgaag actttcataa atatgcgctc
      781 cggaaaaatc aacagactta tacacaatgg atgcaggaaa acacccttaa aaatgatagc
      841 gaagcccacc ctcaagtcct tgagttttca caacaggctt taaagggagg ctccagcaaa
      901 caagccatga atgattggaa tctccttctt cgcagtatcg acattaataa agcggaccgc
      961 gaagttttca cactgcttgc cgagaagctc aagtggcaga cagcgctctg tcgttatctg
     1021 cttttggatc ctgaacttga aagcattttg gaaaatccag acctcgcgcg aaagtgtcta
     1081 tcccaggctt cgtcggtgct ctcaactttg tctaacgtac tgaatgtcgg agatttactt
     1141 gtcgtcgatg ggaaagtttt taatcgtcac cagccgccta caaaattgat gacaggaact
     1201 tatcaatggc gaattatttc gaatctgcaa gaggaccgtc gctttgtggg aacggcagag
     1261 gagtttttaa aacaaaattt tgttctgcag aactgggtga gcggcgactg cggtgattat
     1321 aaattcgatc acaaggattt tagtcttctt ttgcaatctc agatttactt caacgaagct
     1381 tgtataagtc ccgctatgcc tccggagaaa aacttttcta cttggaccgc cgaacacaag
     1441 aagcttattt gggggctcgg cattctggct gcggggataa cggcttttca gcttaaagat
     1501 aaaacactgg ttatcactag gccgtagaaa atgagctgat gctgtttaca atatattttt
     1561 gccatcaaag acgagttcat gcagtttttg atcttcgaaa actgtgtagg tttctccgat
     1621 agtttctccc cgccatgaat gaaattccgc gatctcgcct tggagggctg ggatttttgc
     1681 tcgtgcactt tcggtaaggg ctttttgatt gtcccaaaat gaggatctgg caacgggaga
     1741 atcgcttcca aaataaagtg gaataccgga acgagagagg gttccccaag gaaaggcata
     1801 cttataaaga tcgccgagtt tttctttgag ccatacgcga tcactcagcc aatggcaagg
     1861 ttgcatatgg caacgaacat gcaaggcctt taatttctga acggtttctg gtcttaaaac
     1921 ttcaacgtgt tccagattca accagcccgt gaactgattc gtcgccatca cttggcgtgc
     1981 ggccagaacc acttgatggg cagcctcatc gccaatggta tgaaccgcca cttcgaaacc
     2041 tttggaccaa gtttttttaa ggagagtttg caggtcctct gccgtccacg aaaatttgta
     2101 attcacacaa ttgcattttg aaagccaagc ggtatcggaa cctaaagtgc catcaaagaa
     2161 gatcttaatc ccctgggctt ttaagtgcgg agtttcttgc gactttgctt tttctaaaag
     2221 agccaacacc tcatcgatgt ccttaggatg ctcgcaaaga aaattctctt gaacatacaa
     2281 agtgagttct cccgccagtt ctagatctag agcggctcgc cactgttgct cgtcacaggt
     2341 catatcacga atatgtgtga aacctgcttg attgaaaata gcgcaaccct gtttgaggga
     2401 ttggcgaatt tgcatatcag aaagttttgg caaagaggag taagctttaa agtgttcttc
     2461 ttcctgcaaa attccaggat gattggcttc aaaaccaagt tgagttaacg ccatagagtt
     2521 cagccacgaa caatggccat cggctctctg gagcatggcc ggaaaatgag ggaagcactg
     2581 atcaagaatt tctttgctcg gaagtttttt atctggccat ttgttatgat cccagccaaa
     2641 accgacaagc cattggccac ggaaatacca aggctccact ttgatttttg cgatatcttc
     2701 ggcgctcgct aaatcataga gttgcaagcc gacggcgatt tgtccggtcc caagccagtg
     2761 aacatgggaa tcgtaaagat gagagacttt aaaaaacata agcccctttg ttttagcaga
     2821 agggctttat ttaattaagt gttaatttga aaactaacgg ctgcccgcag gcaccacaac
     2881 tccgttagga atggttgtgt tcgtggagcc ggagacacca ggcgcaggta ggactccgtt
     2941 accgcctcca ttaaacccac caccaatgct tatgccgcca ccaatgctga cttgaccgcc
     3001 gagaccgccg ccaagatagc cagaggaagc tccacccatt tggacttgtt ggattctata
     3061 catcaagttt tgaagttcaa cgttaagccc catcaaagtt tgctgacgag ccatctgatt
     3121 ttgcatctgc tgctgatagt gttgcatgta gagctgctgc tgctgttgtt gcatttgcat
     3181 catggcctgt tgcatttgca tcgagcctaa accaccatcc atcattccag gatagccacc
     3241 cattccagga tagcccccac ccattccagg atagccccca cccattccag ggaagcctcc
     3301 acccattcca gggaagcctc cgcccatttg ggggtagccg aatccaaagc cgccaccgaa
     3361 accaccgccc atcataccag ggtagccgcc tccgccgaag ccgccattaa gtccccaggg
     3421 tcccatgccg ccattgtaca ttccgccacc ccaagggttg ccgaacattc cgttgggata
     3481 cccgaaagga ccattcatgc cgacgttgcc ataaaggccc ccgctcgcac cgaaaggtcc
     3541 catgccaccc atagttccgt aagggaagcc gccaccaccg acagcaccgg cacaaccgaa
     3601 agctccacca ccgactccgc caccaagagc tccataaagt ccatttgcta aaaatggaag
     3661 gccataaccc caagcagggt atgcctgagt gggccaacct aaatctgaat tatagcgagc
     3721 caccatttta ttggtttggt agcccatgta cgtagccatt aaacccactc cgacattggc
     3781 cgcgacaccg gcccaatctg gcccttggct ttccacctga taaccactgc ccctcgccat
     3841 acaagttaag cagatgtccc cttcagttcc ggcttgtctg tcttctttgt agcgagcttt
     3901 tgcttcacgc aaagtctcag gcaactcatc cttgtaacgc tcaatgcgct cttcagattt
     3961 tgcgatgtcg tcctgagttt tttgataatc caaagtgtct ttccgatacg aagctaaacc
     4021 ccgcttacat tcatttggag tataacggcc tcgatcggaa gaggaataag gagcggtact
     4081 acagaccctg ccatcgatac cacctttgac tccttgaata caaacttgag cccattgctg
     4141 aggactaaat cctgaaagag gagtaagatt ggcctgagca gtttctccgc cactttccgc
     4201 tcttgggagt tcttctccat catcactgcc gcgtactttg gaacgtgtct tgggaatttt
     4261 atcagtatca taatctccac aaagccgaga gttatccata tggctaagaa tcgaatcaga
     4321 gtaacgacca tccaccacgt tggtaatcgc atctcttgct ttatcgatgc tcttctccaa
     4381 agtgcggagc ttacgtttca tctcgcgaag ttttgcttct tcgtcagctt ttgctctttt
     4441 gatttcattg taatcttcca ggtaactaga agctccttcg ccagcctgag cattgtaggg
     4501 acaagcttgt tgccccattg cttgaccggt accccaggcc atttgagcat ttgcggacag
     4561 agaaggcaga gtcaatgtcg acactgctaa ggctgccaat cgcgttccga atttcatagt
     4621 tgctctcctg aatcgaaata aaatacactt ctgtacttgc tgatctgtat ttccgctata
     4681 cacagctcat cgtcaggagg agaccaagac ttgactatct tttggtcaaa tctgtgaagg
     4741 aatatggaat ctagaccaaa atccttatgg agtcgtcatc cgtgacaact tatactttcc
     4801 aatcctacac taagttttat ggcgaagcat ttaattcttt gcgtagacga tgaactggac
     4861 aatgtagatg ccttagaaag actctttcgc aagcattaca ctgtactaaa ggcgacctcc
     4921 ggcgcacaag ggttagcaat cctaggcgaa aatccagacg tggctctgat tatctcagat
     4981 cagagaatgc ccaatatgac aggtgtggag tttttggaaa aagcccaact ctctcatcct
     5041 aaggctctgc gcattcttct gactggatac accgatattg aatccgttat tcaatccgtg
     5101 aatcaaggac agatctatcg ttacctgaca aagccctggg actcgacgga cttgctcaac
     5161 actgttgacc acgcgattca aaaatacgag gtttcaaaag agctcgaaaa gaaaaatacg
     5221 gaactcgccg cggctttgca tgagctcaag agtttggata aagcaaagag caattttatg
     5281 attttgatta atcacgagtt gaaaacgccg ctgacttcca ttttaagttt cattggatta
     5341 ttgaatgaga cccagatgga tgatgaacag aaactttttg tgaatcgaat ttctcgggcc
     5401 gctgacaagc ttcgcagcat tatcgatgat gtattgttga ttgttcatgc ggaaaccaat
     5461 caattaaaag tccatctgtc tcctgtttct ttctccgggg tagagaaact cctcaatccc
     5521 gaagtcagca atgtgctgat gtacaaacac cagagcttat tagagaaaaa tatggggagt
     5581 tccgtttccg ccgatcgtga attgattcgt caggttctca atcgtctcat tcacaatgcg
     5641 gccaagtttg gctatgacaa tacggaaatc actatagagg ctaccaagaa gaatgagaaa
     5701 attatttact cagtgttcaa caaaggacct cgcatcgaaa gctcggtgat tgataaaatt
     5761 cttcgtccat ttttcataga tgaggacgtc atgaatcatt cgactggtat gggactggga
     5821 ctcaacatct gccaatcaat ccttaatctg caccaatcaa aattaaaaat tgaaaatacc
     5881 gacgagggtg tgtgcgtctc ctttaactta cccgcagttt aatatctctt ccccctcctt
     5941 cttagactcg gacagaacca tccggcctcc gaactccaaa gcctcgttat ttttctctca
     6001 gccgagtttg ccttttgcag aaaagatttt aggggtgtct atgcgatttt tattcattat
     6061 ttttttatct attcaggctc acgcagagcc tttgctattg aaaatcggag acgatcacaa
     6121 actcccctta ccggcgaatc accgagtttg gattcagaat cgcaaaattc tttctgttgc
     6181 cactcgcggc ggtttcttgg tcttgaatgg caacggcgaa ggcttcacca ctttgcaagt
     6241 ggggactaaa agttatcaag ttcaagtcat tcagccattg aaaaagaccc tattgcaaaa
     6301 ttttgaaaag gaacttcacc acatcttagg tcttcgtctg attattaaaa aataccaagt
     6361 aacggtttcc gggaaactct atcgctggga agattggttg cagcttgcga aaattagcga
     6421 agaaaccggc gttacttatg ctatgagtgc cgaaatcccc gcgagccttc aagctaaagc
     6481 tctctcccac tggcaacagg aattttcagt ggcgggcctg ccccccgtgc ctattcattt
     6541 tgcccacccc ctccaggcgc gcttggcaat agacccccag caatttgcaa aatatgaagc
     6601 catcttagga aattttggcg tgctgcttga aaaagactct caggccctgg atatagcgcc
     6661 cgtggtgaaa gtgcaaatca ccgtggctga agtgcgcaga gacttcgcac tcaactacgg
     6721 attgcaatgg ccctcaggat attcggcaac tattctggcg aatggtcaaa aggaattcga
     6781 gaatctgatg ctgaatgcaa ccgcctttga acaccaaggc cgcgggaaaa ttttagcaag
     6841 ccctaatctc ctttgtcgca gcggtaaaga ggccgagttc ctggccggtg gtgagttccc
     6901 gatcaaaatg aaaactttta aaatgcagga cgtggtctgg aaaaaatatg gaattctgct
     6961 caaggtaaag cctaaagcgg attcctccgg gcgtatgagt attgccattg aaactgaggt
     7021 gtctaccatt gacaatagcc gcagcgttga tggcatcccc gggcttctta ccaatcgcat
     7081 ctcgagtcac ttcgaccttg ctcactctca gaccatcgtg ctttcgggtt taatcaagaa
     7141 tgaagaggga aaatccacag aaggacttcc tttgttaagt cgtattccaa tcctaggagc
     7201 ccttttttcc agccgtgact ttaaagaaaa tcgcaccgag ttggtgattc tcgttcggcc
     7261 taccattgta aaagaaaatg ccaccgagcc cccttcagac tcccccgcac atttgagaga
     7321 cttgaaatat gaataataat ctacaaaata tttttaatca gactcaagag agacttcgaa
     7381 aaatatcaga tcgtggctac ggccatgaag actcttggcg cacgcaaaat gccgagaagt
     7441 tcaagcagtc tctggaagaa attctgaagc ctgcaagcga agaaacccgc cgccgaattt
     7501 tggccgaata tttctcctgc gggcccttag aagacatttt gcaggattcg gagatcaccg
     7561 aaatcctcgt taatggacct gcttctattt ggtttgaaaa acaaggacat ttgcaacgac
     7621 atcctgactt ttttctgtca gaagcgagtt atacaaattg cttgcatcga ctttgtgaac
     7681 aggctcattg ccaaagcacg gtagaacatc cagcaacgac tgggcacttt aaggagtttc
     7741 gtttgacgtt ggtgcgccag gagctgactc acaatcatca tcatctttcc ttgcgacggc
     7801 atccaaaaag tccctggaca ttggaaagac ttcaagaaca aaactgggca ggtgaagagg
     7861 atattaaaaa actacggaag cttattgaaa agaagaaaag ttttctcatc gtcggcagca
     7921 ccgggaccgg aaaaacttct ttgattaatg cccttctgca aacgattccg accaatgaaa
     7981 gagctattgt aattgaagac atcccagaga tcgagctgcc aaattccgct tgtatgaaaa
     8041 tgatcacccg cgaagacccc caagggctgc tgaagaccat tgatcaaagt gagctgctca
     8101 ggcactcttt gcgcttacgc cctgatcgaa ttgtgatggg agaaattcgc ggcgctgaag
     8161 ccaaagatct tttaatggct ttagccactg gtcatgatgg aagctttggg acattgcatg
     8221 ccaacgatcc gcggcaagct ttgattcgtt tagaaatgct tattcaaatg ggcgcgccac
     8281 agtggaatct tcaagccgta agacgactga tacacttaag tttggattgt atcctcatgg
     8341 tcgaaaaaac tcctgaagga aatagaaaat tccagggggc ctacaaaata tgctccctgg
     8401 aagaaaacgg ttttttaatt gaaaaagtct aaataggaga gagcgggcgc tcttcttcag
     8461 ccagatttct catttttgct ggagtgactg gaaagtacga ctggtcctct tccattcttt
     8521 tcaatgtttc ttccgacctt ggtccttcgc cttctttagc cacagtcctt gggttaaaga
     8581 atttctccgc caattcatcg acgactcggg cccgcgcccc ttctttgaaa ttcttataag
     8641 ttgatggctc agcgctacga ccattcgcca gggaactgag acttacacgg tcctggatat
     8701 ttaaaggttt ttgtgtaggt cggccataat cgggagctcc agggcgaact ggttgtgagc
     8761 tttcgacgtc ggtagctacg acccttctgc tgggcggtaa tatacgactg atcttttcca
     8821 attctcacct ccatgtgagt tcatatctcc gtatcggtct gaaacgttta aacttgagac
     8881 gtttctcgga gacttctgta aaactagact ttaaacgctt ccatttattt cagtcctaat
     8941 ttcgtacttg tggtttgtat atatatgtta ctttaccaaa taaccgatat atttgatatt
     9001 tctaaaatca aatatgctct tgtcggtggc tatgctttag cccttcacgg tcttgtgaga
     9061 gccacaatgg atgtggatct tgttttaaat ttaaagcaaa gtgattttga acaggccgag
     9121 accgcattag gcaaaataga tcttcaatct cgattaccca taagagctca agacatcata
     9181 aaaatgcgta aagagtatat cgaaaataga aatttaatag cctggtcctt tgtggattat
     9241 aaaaatccta caagataggt agatattatc ataactaaag acctaaggga catggaaata
     9301 gaaaaaatct ccgtgggtgg acgaaaaatt aaatgaaaaa aaatagccga tctgccagaa
     9361 ggcacttaac aacgatttcg cctgaagaag ctcctcgctt tttggaagac attagaacaa
     9421 tggcaagtga aatagacgag ccaacgacgg caattagtct aagaattccc ggaaatattc
     9481 taagagctct aaagcttaaa gcgaaggctg atgggaaaaa atatcagtct ttgatagttg
     9541 aatacctaga aagagccttc gcgagaagta agatgctatt ggttatcaag cctaaacaac
     9601 atttttacca aggaataggc tcaccatcgc ggaaaaggcc gccattaggt ccgcctttgg
     9661 gaagagtggc tgcccaaata attcctttaa tcccttgttc tatggaacgt tccgcattcg
     9721 gtccgcccat atccgtgcgc acccaaccgg ggcaaacagc attcacgcaa atatctgaac
     9781 cttgagcctc ggaagcaaaa atatttgtca ccatattcaa agcagtttta gaaattctgt
     9841 aacctgcata ggcggtttgc ggttgagaca gctggctcat tccactggaa acgttaacaa
     9901 tgcgaccata accctcctgt ttcatcagtg ggaatatttt ttgtatcaaa agaaaagggc
     9961 ctaatgtatt cgtagtaaat gttttttgaa tcgtcgaagc ttttgtttta aaaacggagg
    10021 agcttcctcc atcttcactg tcagccatga tcccggcatt atttacaagt acatctacaa
    10081 aaccgtactc tctgcgaatc tgttccgcaa aatcgttaat gcttttttct tgagaagtat
    10141 caagtgacat tggcacgaca tccaagtctt tcattttcaa ctcattcact tgtttttgag
    10201 ctttatcagg agtgcgcatg gccataatta ctttgtagcc cctttgcgca agagcctcac
    10261 ttatacctaa ccccaatcct cgacttgccc ctgttacgat tgctatcttt ttcatgttaa
    10321 atccttgggt aaatgctttt ctattttaac tcatataagc atcgggtcgc ttcgttgtct
    10381 tccaatttta aatcacgtta ataatatttt ttaaaccaaa aatattatcg tttaaaattc
    10441 acatcgcctt gctaggtttt ttgataggtt ctgttaagga cttggtacta ccaaagtcct
    10501 ggtaagtggg caaccaggct ataaacttgg agttgcgtgc aaggacgaaa attaaaggca
    10561 ggattctaag tccagtttgc ttgccgctta gtttatagtc ttgaaaaagt tcttaagagt
    10621 gaatgaactg agatcatgtg cttaaaagtc agcgatccga gcatttacta tttattactg
    10681 ctgctaatcc tcggtctcat tcatactttt ccgctttgga atctgcgcga aaacctagcc
    10741 ggaggttacg gagaccccat ggcccatgca gccatcgggg gatggtactg tggcaatgtc
    10801 ctgactggta actttaattc aacacagttt atggctcctt ctggagcgga tctctcagga
    10861 aactatgaca gtccatttcc ctttgtttta acctgccctt
//
LOCUS       DATBTE010000076        13354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1442577, whole genome shotgun sequence.
ACCESSION   DATBTE010000076 DATBTE010000000
VERSION     DATBTE010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 13354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13354
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442577"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VIG33_02940"
     CDS             <1..835
                     /locus_tag="VIG33_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tolA protein"
                     /protein_id="HEY8269816.1"
                     /translation="LDKVAQQESDKALQSLLVTNPASAAEFLMENPESLSLVCKTLQD
                     YDTNIKHKAAIKTVMFWGGIAIAGTLLVATGIGGLAVLSGMAAASTLTTVAAGAAIAG
                     TVTAGGEALYSTGEAYGFFAEAQNLRSSAFAEGLTQESLSKADQAKEQVYSELAEAGF
                     SAISILPFAAGIKYIKTAAQASRLGSISKVATEGSKVETDSMRAIAQTLREVSSNDKA
                     LKALEKAQKVLKDEEMGTFLGYLSDLSPSEKKKILKLIEQNPEKVPEAIRSSAKTGVC
                     Q"
     gene            832..1599
                     /locus_tag="VIG33_02945"
     CDS             832..1599
                     /locus_tag="VIG33_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY8269817.1"
                     /translation="MKRWLVILSLLFSVQSFAWSIQEIRRILNPQNTLYEVLQIKPDA
                     SDKEIRTACRRLVRAYHPDLFPTNSREYPVVTEVIKKINLSRKTLLDPRERNAYNSTI
                     PKSRPTKTSVYSQKTESQETSSARKANEARRARELQEAAKARDARESWRVNQANNARW
                     ADSEKWTPKKINKPNKSAPPKEQPVSQQEVKTPDVKAETAAERSSSLTLEEKAKETNP
                     EPKGISKNPYAEQGIKAYEATSKCGTGYFEKFVDVLL"
     gene            complement(1605..1850)
                     /locus_tag="VIG33_02950"
     CDS             complement(1605..1850)
                     /locus_tag="VIG33_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269818.1"
                     /translation="MARIFLFIGAFLFPVILVPLALHYGPVGLGIIAMSVMGMSLVLY
                     TKLVLKEMKPPEKRLYLIGCTMLAGGVFLLLQQMLFA"
     gene            complement(1896..2420)
                     /locus_tag="VIG33_02955"
     CDS             complement(1896..2420)
                     /locus_tag="VIG33_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY8269819.1"
                     /translation="MSVVQNNSEKNKWYLIGILGGMVITMKHLLKNLFNKKKMDTLNY
                     PEEKYEYSNRFKGNHVLTVKKDGSLRCTACMLCATNCPAECIKITAAEHNDPTVEKFP
                     ISYEIDILRCIFCGFCEEACPVDAIRMGPEWQTPAVNGSKFIYDINHLAYRPNLKGGI
                     LSHVDDEERHKSGI"
     gene            complement(2417..4000)
                     /locus_tag="VIG33_02960"
     CDS             complement(2417..4000)
                     /locus_tag="VIG33_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY8269820.1"
                     /translation="MPKCTINGKEIEVKNGTTIIEAMYENKQSIAHYCYHPGLSVAGV
                     CRLCMVEIEGNPRVQIACNTTIAEGMKINNTSAKVKDAVKWGLDFHLINHPLDCPICD
                     QAGECGLQDQYMEFGKYDPEMAEKKQKKHKVVDLGPTVVLDSERCILCSRCVRFTDEV
                     SKTHELGLFNRGDRTEIGTVEGIQLNNKYSLNVVDICPVGALTSKDFRFRQRVWYLKD
                     SETICTGCSTGCSVKVYFNKEGMFRVKPVYNEQVNGHWMCDEGRGIYKFTNKEARLLK
                     AKKGQGGTWTELAPGAAAKEVAPLLKKAGDSLALVLTAQYTVEEYETILSTFVNTFKT
                     KKIFFWINNKESFDSFDGLLLRGDKNPNTKGLLKTLQKYNVTTSWAELKSGLDSGAIK
                     TLVVAGPENQAVFPDLHERIAEFSKAQNLIWMQAGSNEKLESLTGNVTLIPLKTFVEK
                     NGTFINHQGLEQKIKRVTTIVSEALTLDQAAHLISGEEIPVFVPPTTETLVETNRRPD
                     EVMLEHRKKNEFVFRRGTL"
     gene            complement(3981..5270)
                     /gene="nuoF"
                     /locus_tag="VIG33_02965"
     CDS             complement(3981..5270)
                     /gene="nuoF"
                     /locus_tag="VIG33_02965"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEY8269821.1"
                     /translation="METKLLTEFYHLDEFVGLEGYKKHGGYVELAKALKMQPQQIIDE
                     VKASGLRGRGGAGFPTGTKWSFLPKNDEPRYLLCNADEGEPGTFKDRLMMERAPHQLI
                     EGMIISAFAIGSKKSYIYVRGEYRYPISVLEKALREAYDAGLLGKNILGSGFDHDMDV
                     YSGAGAYICGEETGMISSLEGLKGQPKLKPPFPAIQGYLRKPTIVNNVETLAAVKYIL
                     RDGAQAYRKYGTEKSPGTKLFSLSGNVMKPGNYEVPLAYSLIDLINKEGGGMKPGRKL
                     KAVIPGGSSAPVLTAAEVEKATMDYECLAGIGTMLGSGAVIVIDDSQCMVDMLGVLTH
                     FYHHESCGQCTPCREGTGWLNKIVHSILEGKGRLQDIELLEKVADNMKGKTICALSDA
                     AALPVLSFVSKFRDEFEFYVREGRSKVKGTTYAEMHH"
     gene            complement(5270..5746)
                     /locus_tag="VIG33_02970"
     CDS             complement(5270..5746)
                     /locus_tag="VIG33_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY8269822.1"
                     /translation="MMFKLSEEGIKKVKQELARYEVKDSAIIPSLYVAQKENGGWINV
                     EVIHHLSEVMDIPASKINEVFKFYSMFNQEKVGKYHVQVCTNISCALNGSRELANHIC
                     HELKVKFNEISPDGRFTISKVECLGSCGTAPMMQVNEKYYENLTPESAMNLLRGMK"
     gene            complement(5756..7438)
                     /gene="nuoD"
                     /locus_tag="VIG33_02975"
     CDS             complement(5756..7438)
                     /gene="nuoD"
                     /locus_tag="VIG33_02975"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEY8269823.1"
                     /translation="MSKLENLKQDLSSKFNIAPYKFIHAFGDDVLEIPKEEIIPILRY
                     FKTTQRFDFLMDICGVDYPTRAKRFDVVYHLFSSKDASRLRIKCEVGAGEAIDSALSV
                     WVGADWFEREAYDMFGIQFTGHPNLRKILTHHQFVGHPLRKDYEADAQQHCTEALPIH
                     FNNENGVDGSILNDKYVPLNIGPAHPATHGTLRIMVELDGETIVRANPEIGYLHRCFE
                     KMAETHPYNQVIPYTDRLNYCSAPMNNVGYCKAVERLLGVEIPVKAQAMRVVLMELSR
                     IIDHIIAVGTGGVDLGALSAFFHLFSYREKVYTLFEKLCGARLTVSMTRVGGMAQDAP
                     EGWWDDVLAFCKEMRQGVDEISSLMLDNKIFIKRTQGVCPVTAAEAVQWGYTGPLLRA
                     SGVNLDLRKAAPYYGYDALNFDVPVGTNGDIYDRYLVRIEEMKQSIRIIEQVCKNPPG
                     GDYTIRDKGIVLPEKKDVYGNIEGLMNHFMLIIKGLRPPVGELYDATEAANGELGFYL
                     ISDGTANPYRLKVRPPCFAIYQSFPSVVKGAMIADAIATVASMNLIAGELDR"
     gene            complement(7425..7988)
                     /locus_tag="VIG33_02980"
     CDS             complement(7425..7988)
                     /locus_tag="VIG33_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY8269824.1"
                     /translation="MNSADGTVHPKVDQKLATQSLDDMSRSFALTTRLDDLVAWGRKN
                     SLWPMPYGTACCGIELMSVMGPRYDLARFGAEVVRFSPRQADLLLVAGTITEKMAPVI
                     VRIYQQMLEPKYVLSMGACASSGGFYRAYHVLQGVDKVVPVDVYVPGCPPTPEAVLDG
                     LMVLQRMIATHQPRPWKDNWKSPYEQA"
     gene            8198..9679
                     /locus_tag="VIG33_02985"
     CDS             8198..9679
                     /locus_tag="VIG33_02985"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY8269825.1"
                     /translation="MFLHIMTAVMSLSLSAQAAPTESKQGQKKDQKQDLLIKLAPRTT
                     YSMKLFAQFEGKGASVEQLNDQWIHVEGGQLTHKEMQALSKNPAIESIQPNYPIHLLE
                     DYSVRDPLLRAAIMKAAARQGKNIRALDKDKKYTDNPDFISAPQQTPGADPLFNNQWG
                     MKDIDVADAWKVNIGSPEMIVAVIDTGVDYTHEDLVPNLWINTKEIANNGIDDDNNGY
                     VDDTGGWDFSSDDNKPYDLAVEQLQLLTGGNPGHGTHCAGNVAARSDNGVGIAGVAPN
                     VRIMALRFISDKGAGTTAGAIKAIKYAVDNGAKILSNSWGSEGEDPAEGTENQALRDA
                     VQYAQDKGVLFIAAAGNGHKGVGYDNDTDPKPSYPATYTHENIISVAAIDVNDNFGAF
                     SNWGLKTVHIAAPGVNVFSTTVGSEYSDVVLDKYGFHATWDGTSMATPHVAGAAALYW
                     SAHPEKTWREVKAAILGSAKKIPAATGKLVSEGKLDVNALMHY"
     gene            9782..11566
                     /locus_tag="VIG33_02990"
     CDS             9782..11566
                     /locus_tag="VIG33_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8269826.1"
                     /translation="MSATYSSSQENVAETTELFKNPLLHYIRYQPRAFGVGIFLLVIT
                     NSLDGLYPLLLKVGIDQIEKHQPLNDLGRTSLLLVAAICCLGMTRYAWRRNFGKFHTY
                     AMEDMRLRVFKHFTTLGPSFFQKNQVGELMSVVTNDVASFRQAIGPGLLIFLDGVIYL
                     AIILPVMFSLNFTWTWQTLILLPLIPFLINKVMQLIHQNYKIAQDRFSELTGVTQETI
                     GGIRVVKSFSQEEHRTDLFCQASRKLEKANNKVAFIDSLFIPVMEFGVTSGSVILFFI
                     AANDLYLGIVSIGTFVAFHRYIQKMVWPVTALGLGFSYYEKGRASVDRIKEIMIQATD
                     IPDTGSIELQKFDSLEFKNVSFKYPGAEVYALKEISFKIVAGEHVGLMGQVGSGKSTL
                     VNLMLRLYPVQEGRILINDIPIEKYTLKSLRAALTLVTQEVFLFSETVSRNISYGSVE
                     DPDFKTVERMTHVVNLQSEIENLPQKFDSQLGERGVNLSGGQKQRLSIARGLILGSPF
                     LIMDDALSAVDTRTEKIIDEELAKASSRQTRITVAHRLSSLKSTDKIIVLKDGTMEAM
                     GTATELMQVSPSFRTIVSVQSSSEVNHE"
     gene            11559..13352
                     /locus_tag="VIG33_02995"
     CDS             11559..13352
                     /locus_tag="VIG33_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8269827.1"
                     /translation="MNEDIVKTKVTYPIMFQRLWPYTRRHPWMFFSVVLIIAVLTGIS
                     RLIPYIIGYAIDHGVVAKDITLFKTIGLVYLALEFSRTILSFCQTYMFQLLGNRVLYY
                     LREDLIRHTQALPLHYFNKTPTGRTVTRIANDVSALGDLFTDGVINIFINAVTLLAII
                     IAMALISVKLTLITLATAPLFIYAAIALSEKIREILREQKKKLSNINSFLAENLNGIK
                     VVQLYNRSSKNLSIFQGLSDDYRESNMSSVKAYAAMFPIMNCFTAVTISAAIYFGGWI
                     SLEGGLSIGAVVAFMMNAQDFIPPLRDILEKYQQFQNALTSAERIFTLLDEPAEKEIT
                     GSQELNSLRGEIEIRGLNFCYEESLPLVLKNINLKIEAGESIALVGRTGSGKSTFISL
                     LQRFYDAPAKTIEIDGRPIEEISRHAIRSHIGVVQQDNFIFRGTIADNVSLGDQSITL
                     AQVEKALAQTGYLDLLERTGRNLQSTVEERGANLSVGERQLIAFARILAFNPDIFILD
                     EATANIDSESELILQKATHEVMKSRTSIIIAHRLSTVRECDRIVVLREGEIMEVGSHH
                     ELMKQQGLYYQLASTGLSETPQVEENSVEHS"
BASE COUNT         3705 a         3149 c         2922 g         3578 t
ORIGIN      
        1 cctcgataaa gtcgctcaac aagaaagtga taaagcccta caaagtttat tggtcaccaa
       61 tcctgcctcc gccgctgaat ttttaatgga gaaccctgag tctttaagtc ttgtttgtaa
      121 aactttgcag gactatgaca ccaatataaa acacaaggca gccataaaaa cggtgatgtt
      181 ctggggaggc attgccatcg caggcacact gctggttgct accggtatcg gaggcctcgc
      241 ggttctttcg ggaatggcag cagctagcac attgacgact gtcgcagctg gcgctgccat
      301 tgcaggaacc gtcactgcgg gcggtgaagc actttattcc actggcgagg cttatggctt
      361 ttttgctgag gctcaaaatc tgcgttcttc cgcttttgct gaaggactta cgcaggaatc
      421 tttatcaaaa gcggatcaag caaaggagca agtttactcc gaattggccg aagcaggatt
      481 ttccgccatt tcaatccttc cttttgctgc gggaattaaa tatataaaaa ccgcggcaca
      541 agcttcacgt ttaggttcta tttcgaaagt tgcgacggaa ggctcgaaag tggagaccga
      601 ttccatgcga gccatcgccc agaccctcag ggaggtttct tccaatgata aggctttaaa
      661 ggctttagag aaggcgcaaa aagtgctcaa agatgaggag atgggaactt ttctggggta
      721 tctttctgac ttgtcaccct ctgaaaagaa aaagatctta aaacttattg aacaaaatcc
      781 tgagaaagtt cccgaggcta ttcgtagctc cgctaaaacc ggagtctgcc aatgaaaaga
      841 tggctggtaa ttcttagttt gctcttctca gtccaaagct ttgcttggtc catacaagag
      901 attcggcgta ttttgaatcc tcaaaatact ctttatgagg ttttgcaaat aaaaccagat
      961 gcctccgata aagaaatccg aactgcgtgc cgacgcttag tcagagccta tcatcccgac
     1021 ctctttccga ccaattctcg ggaatatccg gtggtcactg aagtgataaa aaagatcaat
     1081 ctctctcgaa aaactttgtt ggatccgcgg gagcggaacg cttataactc gacaataccg
     1141 aaatcacgtc ccacaaaaac ctctgtctac tctcaaaaaa cagaatctca agaaacaagc
     1201 tctgcgagaa aagccaacga agcccggaga gctcgagaat tgcaagaggc tgctaaagcc
     1261 agagacgcta gggaaagctg gcgagttaat caagccaata atgcaagatg ggcagactca
     1321 gaaaaatgga cgccaaagaa gattaataaa ccgaacaaat ctgctccgcc taaggaacaa
     1381 ccagtcagtc aacaagaggt aaaaactccg gacgtaaagg cagagactgc ggcagaacgc
     1441 tcttcgtctt taactttgga ggaaaaagcc aaggaaacaa atccagaacc taaaggaata
     1501 tcaaaaaatc catatgcaga acaggggata aaagcctacg aagctacatc caagtgtggc
     1561 acgggctatt tcgaaaaatt tgtggatgtg cttttataag gcgctcaagc gaaaagcatc
     1621 tgctgaagaa ggagaaaaac tccccccgct aacatcgtac aaccgatcag gtagaggcgt
     1681 ttttccgggg gcttcatttc cttgagaaca agcttagtat aaagcaccaa actcatcccc
     1741 atgaccgaca ttgcaataat acccaagcca acgggcccat agtggagagc caatggcacc
     1801 agaatcacag gaaaaaggaa agctccgata aacaaaaaaa tacgcgccat tcagccctat
     1861 ctttctgcga cgcatccgaa aaaggtacgg cgtacctaaa ttccggattt gtgtctttct
     1921 tcgtcgtcta cgtgggagag gattccgcct tttaagttgg gtctgtaggc taagtggttg
     1981 atgtcgtata tgaatttgga accgttgacg gctggagttt gccactctgg acccatgcgg
     2041 atcgcgtcta cagggcaggc ttcttcacag aaaccacaga aaatacaacg gaggatatca
     2101 atctcgtaag aaatcggaaa cttctctact gtaggatcgt tgtgctcggc tgcagtgatt
     2161 tttatgcatt ccgcaggaca gtttgtggca cacagcatgc acgccgtgca cctaagagaa
     2221 ccgtcttttt taactgtgag gacgtggttc cctttgaaac ggtttgaata ttcgtacttc
     2281 tcctctggat agttgagagt gtccattttc tttttgttga aaagattctt caacaaatgc
     2341 ttcatggtga tgaccatgcc gcctaagata cctatcagat accatttatt tttttctgag
     2401 ttattttgta ctacactcat aaagtacctc ttctgaagac aaactcattc ttctttctat
     2461 gctcaagcat cacttcatct ggacgacggt ttgtttccac taaagtttcg gttgttggag
     2521 gcacaaaaac tggaatttct tctcccgaaa tcaaatgcgc cgcttgatcc aaagttaaag
     2581 cttcagaaac aatcgtagtc actcgcttga ttttttgctc aaggccctga tgattgataa
     2641 acgtcccatt tttttccaca aaagttttta atggaatcaa ggtcacattg cctgttaaag
     2701 actcaagctt ttcgttcgaa cctgcttgca tccagattaa gttttgcgct tttgaaaatt
     2761 cagcgattct ttcgtgcaag tcagggaaaa ccgcttggtt ttctggtcct gccaccacta
     2821 aagttttaat cgctccactg tccaaacctg acttcaactc ggcccaagaa gtcgttacat
     2881 tgtacttctg tagggtcttc aaaagacctt tggtgtttgg atttttatcc ccacgcagga
     2941 gcaagccatc gaaagaatca aaactttctt tgttgttaat ccagaagaag atcttcttcg
     3001 tcttaaaagt atttacgaaa gtgctgagaa tcgtttcata ctcttcgact gtgtattgag
     3061 ccgtcagaac caacgctaaa gaatcaccgg cctttttcag taaaggggca acttctttcg
     3121 ctgcagctcc gggagctaat tctgtccaag ttccaccttg gccttttttt gcctttaaca
     3181 gacgcgcttc tttattggta aacttgtaga tgccgcgacc ttcatcacac atccaatggc
     3241 cgttcacttg ctcattataa accggtttta cgcggaacat gccttcttta ttgaagtaca
     3301 cttttacact gcaacctgta gagcagcccg tgcaaatcgt ttcggaatcc ttgagatacc
     3361 atacccgctg acggaaacgg aaatctttag aagtcaaagc acccaccgga cagatatcca
     3421 ccacattgag ggaatattta ttgttcaatt gaataccttc aacagtgccg atttcagtgc
     3481 gatcgccgcg attgaaaaga cccaactcat gggttttaga aacttcatcc gtgaaacgaa
     3541 cacaacgaga gcagaggatg caacgttctg aatccagaac cactgtcggt cccaaatcca
     3601 caactttatg tttcttttgc tttttctcgg ccatctcagg atcatattta ccgaattcca
     3661 tatattgatc ttgcaaaccg cactcgccgg cttggtcgca aatagggcaa tccaaaggat
     3721 ggttgatcaa atggaaatca agtccccact ttacagcgtc ttttactttg gcagaggtgt
     3781 tattgatttt catgccttca gcgatcgttg tattgcaggc gatctgaacg cggggatttc
     3841 cctcgatctc taccatacag agacgacaca ccccagcgac actcaagccc ggatgatagc
     3901 aatagtgagc gatcgattgt ttattctcgt acatggcttc gatgatggtc gtgccatttt
     3961 taacttcgat ttcttttccg ttaatggtgc atttcggcat acgtcgtacc cttcacttta
     4021 gagcgtcctt cacgaacata gaattcaaat tcatcacgga atttggatac aaaacttaat
     4081 actggcaaag cggctgcatc tgaaagagcg cagatcgttt tacctttcat attgtcagca
     4141 accttctcga gcaattcgat gtcctgcaaa cggcctttgc cttccaagat ggagtgaacg
     4201 attttgttca accagcccgt gccttcgcgg caaggagtgc actgaccgca agattcatga
     4261 tgatagaagt gagttaacac tcctaacata tccaccatgc attgagaatc atcaataacg
     4321 atcacggcgc ctgaaccaag catggttccg atccccgcca ggcattcata atccatcgtc
     4381 gctttttcga cttccgccgc cgtcagtaca ggtgctgaag atcccccagg aatcaccgct
     4441 tttaacttac ggcctggctt cattcctcca ccctctttgt tgatcaaatc aatcaaagaa
     4501 taagccaaag gaacttcgta atttcccggt ttcatgacat taccagaaag tgaaaacaat
     4561 tttgtgcccg gagatttctc ggttccatat ttacggtaag cttgagctcc atcgcgaagg
     4621 atgtatttca ccgccgccaa agtctctacg ttatttacga tcgtcggctt acgcaaataa
     4681 ccttggatcg cggggaaagg aggtttcaac ttcggctgac ccttcagccc ttccaaagag
     4741 gaaatcattc cagtttcttc tccgcaaata taagcacccg ctccactgta gacatccata
     4801 tcgtggtcaa aaccagaccc caggatgttt ttaccaagaa ggcccgcatc gtaagcttcg
     4861 cgaagagctt tttctaaaac agagatggga tagcgatact caccacgcac ataaatatat
     4921 gattttttag aaccaatggc gaaggcagag atgatcatcc cttcgatcaa ttgatgagga
     4981 gcccgctcca tcatcaaacg atctttgaaa gtacccggct caccttcatc cgcattgcaa
     5041 agcaaataac ggggctcatc atttttaggc aggaagctcc atttagttcc cgtggggaaa
     5101 ccggcaccgc cacgcccgcg aaggcccgaa gctttcactt catcgatgat ctgttggggc
     5161 tgcatcttca aggcttttgc cagctctacg taaccaccgt gttttttata gccctcaaga
     5221 cccacgaact catcaaggtg ataaaattct gttaataatt ttgtttccat tatttcatcc
     5281 ctctcaacaa attcatcgca gattcaggag tcaaattttc gtaatacttt tcattcacct
     5341 gcatcatcgg agccgtcccg caagaaccta gacattccac tttagaaata gtgaaacgtc
     5401 catctggact gatctcattg aatttcactt tcaattcatg acaaatgtgg ttggcaagtt
     5461 cacggcttcc gttcaaggca caggaaatat tcgtgcatac ttgaacatgg tatttaccca
     5521 ctttttcttg attgaacatg ctgtagaatt tgaaaacttc gttgattttg ctggctggaa
     5581 tatccatcac ttcagagagg tgatggatga cttccacatt tatccagccg ccgttttctt
     5641 tctgagccac atataaactg gggataatcg cagaatcctt cacttcgtaa cgtgcaagtt
     5701 cctgcttaac ttttttaatt ccctcttcag acaacttaaa catcattcac tccgactatc
     5761 tgtctaattc accggcaata agattcatac ttgcgaccgt cgcaatggca tcggcgatca
     5821 tcgccccctt cactacggaa gggaatgatt ggtagatggc aaagcatgga ggacgcactt
     5881 tcaagcgata tggatttgcg gtcccatcac tgattaaata gaagcccaat tcgccattcg
     5941 ccgcctcagt ggcatcgtaa agttcaccga ccggaggacg aagacctttg atgatcaaca
     6001 taaagtgatt catcaagcct tcgatattgc cataaacatc ctttttctct ggaagaacaa
     6061 tgcctttgtc gcggatcgtg taatccccac ccggaggatt tttacaaacc tgctcgataa
     6121 tacgaatact ttgtttcatc tcttcgatac gcaccagata acggtcataa atatcaccat
     6181 tggtgcccac tggaacatcg aagttcaaag catcataacc ataataagga gcggctttac
     6241 gtaaatcgag gttcacccct gaggcgcgca gaagagggcc tgtataaccc cactgaactg
     6301 cttctgcagc ggtcacgggg cagacacctt gagttctttt gatgaaaatt ttattgtcca
     6361 acatcagaga tgaaatttca tccactcctt gtcgcatttc cttacagaag gcaaggacgt
     6421 catcccacca accttcaggg gcatcctggg ccataccgcc gactcgggtc attgacaccg
     6481 tcaagcgcgc tccacaaagt ttttcaaaca gagtgtaaac cttttcacga taggaaaaga
     6541 gatggaagaa ggccgataaa gcccccaaat ccaccccgcc tgttccaaca gcgataatat
     6601 ggtcaataat ccgtgagagt tccatcaaca cgacccgcat tgcctgggct tttacgggaa
     6661 tttcgactcc taaaagtctt tccactgctt tgcagtagcc cacattattc atgggagctg
     6721 agcaataatt caaacggtcc gtgtaaggaa tcacctgatt ataaggatga gtctcggcca
     6781 tcttttcaaa acagcgatgc aaataaccga tctctgggtt ggcccgaaca atagtctccc
     6841 cgtcaagttc caccataata cgaagagttc catgggtcgc tggatgagcg ggaccgatat
     6901 ttaaaggaac atatttatcg ttaagaatag agccatcaac cccattttca ttattgaaat
     6961 ggatgggtaa ggcttctgtg cagtgctgct gagcatccgc ttcatagtct ttgcgcagag
     7021 gatggccaac aaactgatga tgtgtcaaaa tcttgcgaag attgggatgg ccagtaaatt
     7081 gaataccaaa catatcatag gcttcgcgtt caaaccaatc ggcgccaacc catacgctga
     7141 gagcggaatc gatggcttcg cctgcaccca cctcacattt aatacgcaag cgcgaggcat
     7201 cttttgaaga gaataagtga tagaccacat caaaacgttt tgcccgagtg ggatagtcta
     7261 ctccgcaaat atccatcaag aaatcaaaac gctgagtcgt tttaaaataa cgaagaatgg
     7321 gaataatttc ttcttttgga atctccaaaa catcatcacc aaaggcatga atgaatttat
     7381 aaggagcgat attaaacttt gaggagagat cctgttttaa attttcaagc ttgctcataa
     7441 ggtgacttcc aattgtcctt ccaaggacgt ggttgatgag tggcaatcat tctttgcaaa
     7501 accatgagac catccaaaac cgcctccgga gtcggaggac accctggaac atagacatca
     7561 acagggacga ctttgtctac gccctgaagc acatgataag cgcggtagaa gccaccagag
     7621 cttgcacagg ctcccataga aagcacatat ttaggttcaa gcatctgttg atagatacgc
     7681 acaatcacgg gcgccatttt ttctgtaatg gtgccggcca caagaagtaa atctgcttgg
     7741 cgaggagaaa aacgcacgac ctccgcgccg aaacgagcca ggtcataacg tggacccatc
     7801 acagacataa gctcaatccc acaacaagca gttccataag gcatcggcca tagtgaattt
     7861 tttcttcccc acgctaccaa atcatccaga cgagtggtca gcgcgaaact acgtgacata
     7921 tcatccaagg attgagtagc taacttctga tcaaccttag ggtgaacagt tccatcagct
     7981 gaattcattt tgacactcca ttttcatttg actctcactt agttacagaa aatcccaagc
     8041 tatgacaact agatagataa tagggagagt ggcttttatt tattcaacgc gtgcgctcaa
     8101 ttaaatagat ttgcaattga ttctagctag agcttatccc tagaattgga gttcaagacc
     8161 aacgaaggtc tagattgtaa catgtgaggg ggaatccatg tttttgcaca ttatgacagc
     8221 agttatgagt ttatcattga gcgcgcaagc agctcccacg gaatcaaaac aaggacaaaa
     8281 aaaagaccaa aagcaagatc tcttgattaa acttgctcca cgaaccactt attcaatgaa
     8341 attgtttgct caatttgaag gcaaaggggc ttctgttgag caattaaatg atcaatggat
     8401 ccacgtagaa ggtggacaac tgactcataa ggaaatgcag gcactgtcga agaatccagc
     8461 tattgagtct attcaaccga actatcctat tcatttgctc gaggattatt cagtccgcga
     8521 tcctttactg cgcgcagcga ttatgaaagc cgctgctcgc caaggaaaaa atataagagc
     8581 tcttgataag gacaaaaaat acacagacaa cccggatttt attagcgcac cgcaacagac
     8641 ccctggagct gatcctttgt tcaataatca atggggcatg aaagacatcg atgtggctga
     8701 cgcttggaaa gtcaacattg gatctcctga gatgatcgtc gcggtgattg atacgggcgt
     8761 ggattacaca catgaagatt tagtgccgaa tctttggatc aacactaaag aaatcgcgaa
     8821 caatggaatt gacgacgata acaatggtta tgttgatgat accgggggct gggacttttc
     8881 ctctgatgac aacaaacctt atgacttggc tgtagagcaa ctccaattgt tgactggcgg
     8941 aaatccaggt cacgggactc attgcgcagg aaatgtggct gctcgtagcg acaatggggt
     9001 cggtatcgca ggtgttgctc cgaacgtgag aatcatggct ttgcgcttta tctctgataa
     9061 aggtgccggt acgactgctg gcgcgatcaa agctattaag tatgcggtcg ataatggagc
     9121 gaaaattctt tcgaactctt ggggctctga aggcgaagac ccagcagaag gcactgaaaa
     9181 tcaggcactt cgtgatgcgg ttcaatatgc gcaagataaa ggtgtccttt tcatcgcagc
     9241 ggcaggcaac ggccataaag gtgtcggtta cgacaatgat acagatccta agccctctta
     9301 tcctgcaact tatactcatg agaacatcat ttccgtagcg gctattgacg tgaatgacaa
     9361 cttcggcgct ttctcaaact gggggcttaa aactgttcat atcgccgctc ctggggtaaa
     9421 cgttttctct acgactgtgg gaagtgaata ctctgacgta gtccttgata agtatggttt
     9481 tcatgcaact tgggatggaa cctctatggc gactccccat gtagctggtg cggcggctct
     9541 ttactggtca gctcaccctg agaaaacttg gagagaggtc aaagcggcta ttttaggctc
     9601 tgctaagaaa attcctgcag ctactggcaa attggtcagc gaaggaaaac tcgatgtgaa
     9661 tgcccttatg cactactaga tttggtttga agataaacta aagaaaaaaa gggtggagtt
     9721 ttccaccctt ttttttatga aagtcttgct gtgagtctat ttacgatgat attagcaagc
     9781 tatgtcggcc acttatagtt cctctcaaga aaatgttgca gaaaccactg aactttttaa
     9841 aaaccctctc cttcattaca tccggtatca accccgggcc tttggagtgg gaatttttct
     9901 cttggtgatt accaactcct tggatggtct ttatcctctt ctattaaagg ttggaattga
     9961 tcaaatcgaa aaacatcagc ctctcaatga cttggggcgt accagtcttc tcttggttgc
    10021 ggctatttgt tgtctgggca tgacccgcta tgcatggcgc cgcaattttg gaaaatttca
    10081 tacctatgca atggaagata tgcgtctgcg ggttttcaaa cacttcacaa ccttgggacc
    10141 tagtttcttt cagaaaaatc aagtcggcga actcatgagt gtggtcacaa acgacgtcgc
    10201 ttctttccgt caagccatcg gccccggtct cttgattttc ctcgatggcg tgatttatct
    10261 ggcgatcatc ttgccggtga tgttctcttt gaactttacc tggacttggc aaactttgat
    10321 cttattgccg ttgattcctt ttctgatcaa caaagtgatg cagttgatcc atcagaatta
    10381 taaaatcgcc caggaccgct tttctgagct caccggagtg actcaggaaa cgatcggcgg
    10441 gattcgcgtg gtgaaatcct tctcgcagga agagcatcgc acggatctct tttgccaggc
    10501 gagccgaaaa ttggaaaagg ccaataataa agtggccttt attgattctc tttttatacc
    10561 ggtgatggag tttggggtta ccagcggctc ggtgatttta ttttttatcg ccgccaacga
    10621 tctttactta ggaatcgtca gcatcggaac cttcgtcgcc ttccatcgct atattcaaaa
    10681 aatggtttgg cccgtgacag ccttgggctt gggtttttct tactatgaga aaggccgcgc
    10741 ttccgttgac cgcattaaag agatcatgat tcaagccaca gacattcccg acaccggctc
    10801 gattgaactg caaaaatttg attcattgga gttcaaaaat gtttctttta aatatccagg
    10861 agccgaagtc tacgccctca aagaaatttc ttttaaaatt gttgccggcg aacatgtggg
    10921 actcatgggg caagtcggct caggaaaaag caccttggtg aatttaatgc tgaggctcta
    10981 tcctgttcag gaaggccgga tcttaatcaa cgacatacct attgaaaaat acacgttaaa
    11041 aagtcttcgc gccgctttga ctttagtcac tcaagaagtc tttctcttca gcgagacggt
    11101 ctcgagaaat attagctatg ggtctgttga agacccagac tttaaaacag tggagcgcat
    11161 gacccacgtg gtaaacctgc aaagtgaaat tgaaaacctg ccgcagaagt ttgactcgca
    11221 actgggcgag cgcggagtga atctctcggg aggacaaaaa caacgcctga gtatcgctcg
    11281 cggtttaatt ttaggctccc cttttcttat tatggatgat gccctcagtg ccgtggacac
    11341 tcgcactgaa aagatcattg atgaagagct ggcgaaagcc agctcacgac agacccgcat
    11401 caccgtggct caccgtctga gctctttgaa aagtacggat aaaatcattg tacttaagga
    11461 tggcaccatg gaagccatgg gtactgccac tgagctcatg caagtaagtc cgagcttccg
    11521 caccattgtc agcgttcaat caagctcgga ggtgaatcat gaatgaagat attgtaaaga
    11581 ccaaggtcac ctacccgatc atgtttcaac ggctgtggcc ctatactcgc cgacatcctt
    11641 ggatgttctt cagcgtcgtt ttgatcatcg cggtgctcac cggcatttct cgtctgattc
    11701 cttatattat cggctacgcc atcgatcatg gcgttgtggc caaagatatc actttgttta
    11761 aaaccattgg gttggtttat ctggctctgg agttttcgcg gacgatttta agcttctgcc
    11821 agacttatat gtttcaattg ctcggaaaca gagtgctgta ttatctgcgc gaggatttga
    11881 ttcgccacac acaagcgctg cctttgcact attttaataa aacgcccaca ggtcgaactg
    11941 tgacccggat tgcgaatgat gtcagcgccc ttggcgatct gtttaccgat ggggttatca
    12001 atatatttat taatgcggtg accctgctgg ctattattat agcgatggct ttgatttccg
    12061 taaaattgac cttgattact ctggcgaccg cgcctttgtt tatatatgcg gctatcgcct
    12121 tgagtgaaaa aatccgggaa attctccgtg aacaaaaaaa gaagctttcc aatatcaact
    12181 cttttttggc ggaaaacctc aacggcatca aagtggtgca actttataat cggtcctcta
    12241 aaaacctttc gatctttcaa gggctttcgg atgactatcg agagagcaat atgagttcgg
    12301 tgaaggccta tgccgcgatg tttccgatca tgaattgctt caccgctgtc accatttccg
    12361 ccgcgatata tttcggaggc tggatcagct tggagggcgg actcagcatt ggtgccgtgg
    12421 tggcctttat gatgaacgcc caggatttta tccctccttt gcgggatatt ttagaaaaat
    12481 accagcaatt ccagaatgcc ctcaccagcg cggaacgtat ttttacttta ttggatgaac
    12541 ctgcggaaaa agagatcact ggcagtcagg aactcaacag tctgcgcggt gaaatcgaaa
    12601 tcaggggtct taatttttgt tacgaagagt cccttccatt ggtgttaaag aatataaatt
    12661 taaaaatcga agctggtgaa tccattgcct tggtgggccg tactggaagc ggtaaaagca
    12721 cttttatttc tttgctgcaa cgtttttacg atgctcctgc taaaaccatc gagatcgatg
    12781 gacgccccat tgaggaaatc agccgtcacg ccatccgttc tcacatcggc gtggttcaac
    12841 aggataattt tattttcaga ggaaccattg ctgacaacgt cagcctcggt gatcaaagca
    12901 tcaccttggc tcaggttgaa aaggcactgg ctcaaacggg ttatcttgat ctgcttgagc
    12961 gcaccggccg aaacttacaa tccaccgtgg aagagcgcgg tgcaaattta tccgtgggcg
    13021 aaagacagtt gatcgccttt gcgcgcatct tggcttttaa cccggatatt ttcattctcg
    13081 atgaggccac ggcgaacatt gattcagaaa gtgaattgat tctgcaaaaa gccactcacg
    13141 aagtcatgaa aagccgcacc agtattatta ttgcccatag gctttctacc gtgcgcgaat
    13201 gcgatcgcat cgtggtttta cgtgaagggg aaatcatgga agtcggttcg catcatgaac
    13261 tgatgaagca acaaggattg tattatcaat tggccagcac cggattaagc gaaacccctc
    13321 aagtcgaaga aaacagcgtc gaacatagct gaca
//
LOCUS       DATBTE010000077         6531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1032510, whole genome shotgun sequence.
ACCESSION   DATBTE010000077 DATBTE010000000
VERSION     DATBTE010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032510"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VIG33_03000"
     CDS             <1..320
                     /locus_tag="VIG33_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease spb1"
                     /protein_id="HEY8269828.1"
                     /translation="FKNSQICIDLIWERKPTETEFGSFVLEFYNPDNRSQFIDPPGTL
                     SVHIRMPSTDQGSYPVNINKLSAGQYRVSKVFFTMHGDWEIQLKLLNGSNILDQAVNP
                     VHF"
     gene            336..411
                     /locus_tag="VIG33_03005"
     tRNA            336..411
                     /locus_tag="VIG33_03005"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:370..372,aa:Arg,seq:cct)
     gene            complement(390..2111)
                     /locus_tag="VIG33_03010"
     CDS             complement(390..2111)
                     /locus_tag="VIG33_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY8269829.1"
                     /translation="MSHKIGAYLRVSTEEQAAAVEGSLDNQRYRLKSFIDIKNTQEKS
                     WGQIVEFYIDDGFSAKDTKRPAYQRMMADIKKGKINLILITDLSRLSRNIHDFSNLLN
                     DLDRVKATFFSIKEQFDTSSPSGKMMIYNLINLAQFEREQVSERVSLGVHARAMRGLL
                     NGGRPILGYDKHADKPGVYILNAKEADQVRQIFKSFLETGSRAKTIQRLNDLGIKPKL
                     TGKVGKLKLNDKWSSQTLGHLLNSAVYIGCHEVNRRQKSKDQETLKPFQRYQVVNASW
                     PAIITEETFYRTQALLEEAKEFERARLEGAENRVYILSGTLRCGDCGHPLVGQSAHGS
                     KGVLRYYGHTYAGTKHGCPIQRVSAEAIEKAVLDHLKKGLNTAGYFKGIEENLSESMK
                     VTSLDNAREVRRVKQRLQELESQLGNMFLVQTQQKMSGEALRMLMETIEKFAKEKTVL
                     STYLEELSRQDSKGDFVRASVNSIKERLDEFAKGFSKANGNLKKRLIRRALKQLVLTR
                     EGLSIFFHLADGEEISGHKLQLVRDETSKAEKTKALFLTKRASGDDTNLSILRSDIGK
                     HGDLTPS"
     gene            complement(2101..2301)
                     /locus_tag="VIG33_03015"
     CDS             complement(2101..2301)
                     /locus_tag="VIG33_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269830.1"
                     /translation="MSEKQKRNESKTMNEVIVIKVEFVKDASSHSAKRSKEVTDIISQ
                     MIQLSHKRGRPSQQQQEMEDVA"
     gene            complement(2298..2552)
                     /locus_tag="VIG33_03020"
     CDS             complement(2298..2552)
                     /locus_tag="VIG33_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269831.1"
                     /translation="MSPKRFKDIRVSTGLTQDAFGKYLGLAKLSVSQFETGFRKPGKQ
                     LVLLMSYIELLNDRKRHEFLKVLREIVEKEFVSSSGKVRT"
     gene            complement(2549..2965)
                     /locus_tag="VIG33_03025"
     CDS             complement(2549..2965)
                     /locus_tag="VIG33_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269832.1"
                     /translation="MNTEEKTERQEKENKKEKNEIQITIGTLAEMKVSNLLDCVNDGF
                     ELGRVSRKDLASYLLLKAVEKFNDEDVVKVRGQTLTDLSLLEYELKQARETGFVPEDL
                     REFLRKKANLALGSKKSKKTGQNKNSNAICPNVEAD"
     gene            complement(3600..3995)
                     /locus_tag="VIG33_03030"
     CDS             complement(3600..3995)
                     /locus_tag="VIG33_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269833.1"
                     /translation="MKTAILSLLLISGFAHASQDIPAPKPLECTLDKSVFSNKVIGEV
                     EITDNDGHGHLEKRFEMKEYPGYTMIATSFTADFKGGYADGVQIRIKNPQQANTELGE
                     SMITITGLSMAELWFTIEGKLAAARCYVK"
     gene            complement(4717..5955)
                     /locus_tag="VIG33_03035"
     CDS             complement(4717..5955)
                     /locus_tag="VIG33_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY8269834.1"
                     /translation="MKYLLSLIFSFYAFASAAINVPAESLKESIIVLDGDDGCGTGFV
                     LSSGKIITNAHVITQKCNQSLCPNVKISRASALGATPVSIGLVDLQIIATFPKTDLAI
                     LKPTRNLNIKGISGKIRSPVDDEEIFTIGFPNCEFLNIQMGNVSNSTASEFGLNFTIQ
                     KGQSGSPVFNKYFDIVGLVYQGNSFDLGKATRVDHLGNDFEAIDSQSGLIDLQNLYRS
                     ALDAINSPDTYKQITSINQERDIPEVETQLALSGQCPALKDYWIWTHAGFPAHVPAPI
                     IDAKALSTKCYEVFQQAVEYQTYKILLLRDYVDFFRDIDKLEALKTLSPMAYFAEIQY
                     YQSKNSVNLDSTLIGKLKKKVQTYGKLVSGFTGVLCIFFVLFFIFKFLFSRFRTFRRF
                     FVGAFILCAICTLSLLMLLT"
BASE COUNT         1811 a         1450 c         1294 g         1976 t
ORIGIN      
        1 aatttaaaaa ctcccaaatt tgcattgatt tgatttggga aagaaagccg acggagaccg
       61 agtttggctc cttcgtttta gagttctaca atcccgataa tcgcagccag tttatagatc
      121 caccgggcac tctgagcgtc catatccgga tgcccagcac ggaccaagga tcttacccgg
      181 tgaacatcaa caagctctct gcggggcaat accgagtctc taaagtgttt ttcacgatgc
      241 atggcgattg ggaaattcaa ttgaaacttc tcaacggtag taatattctc gatcaagcgg
      301 tgaacccagt tcacttctaa ataatcttaa ttatcgacct catagctcaa gtggatagag
      361 ctaccgtttc ctaaacggta gatccccgtt caactcgggg tgaggtcacc atgttttcca
      421 atatccgaac gcaggatcga cagattagta tcatcgccag aagccctttt cgtaagaaag
      481 agggcttttg ttttttccgc cttagaggtc tcatctctaa ccaattggag cttatgcccc
      541 gaaatctctt ccccatcagc taaatggaaa aatattgaaa gaccttctct ggttaaaacg
      601 agctgcttaa gagcacgacg aatcagtctt ttctttagat tgccattggc ttttgaaaac
      661 cccttcgcaa actcatcgag ccgttcctta attgaattaa cagaagctct gacaaaatca
      721 cctttgctgt cttggcgact caactcctca aggtaagtgg aaagaacagt tttttctttc
      781 gcaaacttct caattgtttc catcaacatt cttaaagctt caccactcat tttctgctgg
      841 gtttgaacta aaaacatatt gcccagttga gactccagtt cctggagacg ctgcttcact
      901 cggcgcacct ctcgtgcatt atcaagagag gttactttca ttgactcact gagattttct
      961 tcaatccctt tgaaatatcc cgccgtgttt aatccttttt tcaaatgatc cagcaccgct
     1021 ttttcaatgg cctcagcaga gacccgctgt ataggacaac catgctttgt cccagcataa
     1081 gtatgaccat aataacggag aacccctttg ctcccgtgag cggactgccc caccaaaggg
     1141 tgcccacagt cgccacatct taaagttccc gagagaatat aaacccgatt ctcagcccct
     1201 tccaaacgag ctcgttcaaa ttcctttgct tcctcaagca gggcttgagt tcgataaaac
     1261 gtttcttcag ttataattgc aggccacgaa gcattgacca cttgataacg ttgaaatggt
     1321 tttaatgttt cctgatcttt actcttttgg cggcgattga cttcgtgaca gccaatgtac
     1381 actgccgaat tgagcaggtg cccaagggtt tgactgctcc atttgtcatt caacttgagt
     1441 ttaccgactt tcccagtcaa tttaggtttt atcccaaggt cgttaagccg ctgaattgtt
     1501 ttagcgcgtg agcccgtttc caaaaaggac ttgaagatct gcctcacttg atcggcctct
     1561 ttggcattaa gaatataaac acccggcttg tcggcgtgct tatcatagcc taatattgga
     1621 cgccctccat tcaatagccc gcgcattgct ctggcgtgaa ctcccaaaga cactcgctct
     1681 gaaacctgtt ctctctcaaa ttgagccaag ttaatgagat tataaatcat catcttccca
     1741 gagggtgagc tggtatcgaa ttgttctttg atagaaaaga acgtcgcttt aacacgatct
     1801 aggtcgttta ataaatttga aaaatcatga atattacgag aaagacgaga gagatctgtg
     1861 ataagaatga gattgatctt acctttttta atatccgcca tcattctctg ataagcaggg
     1921 cgctttgtgt cttttgcaga gaaaccatca tcaatataga attcaacaat ttgcccccag
     1981 gatttctctt gagtgttttt gatgtcgata aaacttttaa gacgatatct ttgattatct
     2041 aacgaacctt caacggcagc agcctgctct tccgtactta ctcttaaata tgccccaatt
     2101 ttatgcgaca tcttccatct cctgttgttg ctgcgaaggt cttccacgtt tatgcgaaag
     2161 ttgaatcatc tgagaaataa tatccgtcac ttctttagat ctttttgctg agtgagatga
     2221 tgcgtcctta acaaattcaa ccttgatcac gatcacttcg ttcatggttt tcgactcatt
     2281 tcttttttgc ttctcactca cgtccgaacc ttcccagacg aacttacaaa ctctttttca
     2341 acaatttccc tcagaacttt taaaaactca tgtcttttac ggtcattcaa caactcgatg
     2401 taactcataa gaagaacaag ttgttttcca ggtttacgaa agcccgtttc aaattgactg
     2461 actgatagct tcgcaagtcc gaggtacttg ccgaatgcat cctgagtaag tcctgtactc
     2521 actctaatat ctttaaatct cttaggactc aatcggcctc cacgtttgga catatagcat
     2581 tgctattttt attttgtcca gtcttttttg atttcttgga gccaagcgcc aagtttgctt
     2641 tcttgcgaag gaattctcga agatcctctg gaacaaatcc cgtttctcga gcttgtttaa
     2701 gctcatattc caaaagactc aagtcagtca gcgtttgacc acgaacctta acgacatcct
     2761 catcattgaa cttttcaact gcctttaata acaaatagct cgcgagatct ttgcgggaaa
     2821 cccgtcccaa ctcaaatccg tcattaacgc aatcaagcag attggagacc ttcatttctg
     2881 ccaatgtacc tatggtaatt tgtatttcat ttttctcttt tttattttct ttttcctgtc
     2941 gttccgtttt ttcttctgta ttcatatccc cacctcctca tttatattat ttttaactct
     3001 aaaccttctt ccacccaacc ccggcccccg gttcgtgacc accttcaacc aactactcgt
     3061 tgggtggggg tggtcacgaa cagctctgct gagggggccg gggttgggtg gaccactatc
     3121 tagtggattt ttcctctgat ttttcatgca gcttccttgg atttcatgga taatccgtgg
     3181 acgactttcc atgttttatc catgttattt tgccagtctg attctggccg ctggaacctt
     3241 ggccgctttc caaagagttc tccttgtaat tttcgcttat ggaaaattgc acgagacggg
     3301 attcgctcgg ttgtatgatt ggaacgtaag tttaatggat actcttgagt tgaggaggta
     3361 ttaatataga ttattttaaa ctattttttt aaagacgatg acattttggc tagctcgcaa
     3421 aatgaaccct gtagtgcact ttagcggtca gaaatctgtc cttttaatca tgaaaatcat
     3481 taagccaaat acttattgat cattcacgat atctaaaaga ttaaaagcca gcataaatca
     3541 atactggctc tggaaaagat tgtagtattt ttggaacgcg aagtaaagtt tcgtagttgc
     3601 tacttcacgt aacagcgggc agctgcgagt ttcccctcaa tcgtgaacca caattcagcc
     3661 atggaaaggc cagtgatcgt tatcatggat tccccaagtt ccgtattggc ttgctgagga
     3721 ttcttaatac ggatttgaac accatccgcg tatccgccct taaagtctgc cgtaaatgag
     3781 gtcgcaatca tggtatatcc agggtattcc ttcatctcaa aacgcttttc gagatgaccg
     3841 tgaccatcat tgtctgtgat ttcgacctcg ccgataacct tattgctgaa caccgattta
     3901 tcaagggtac attccaaagg ctttggcgcc ggaatatctt gcgaagcatg ggcgaatccc
     3961 gagatcaaca gcagggaaag aatggcagtt ttcatatgtg agcctcctgt acattttttt
     4021 tacaagcggc tccaatttgg aggtccgaac tacgcctctg cgttttttag aagggggtag
     4081 tttgtgccaa ctgttgatag aaaatatgcc agacctaggt ctattggttt ttattaaaat
     4141 cagtattgct ctatcctaat gttttcattg gacttctgat tgtacccaat cagaagtaaa
     4201 gtcgcgctac gatttttaat ataaataata agtaccttcg cgacttatga tagccatatc
     4261 ttcgtctagt tggaaattta agacccttct ataaaacgga gagccccgaa ctactcgaat
     4321 ctcctgccgc cttcgtgtta ttgttccatg tggtatcaca gcaagatatg ttccaaaaag
     4381 gaatgtctag agacttgatt gcggtcggcc cggctctcgc ttattggtct gatgattttg
     4441 acactgagaa gccagttttg cacggcccca ttcagtatta ttggccagga catctagatc
     4501 gcaggcatta ggttgcagat agctcagtaa aaaaattaag gagggagcgg atccatacga
     4561 tttttaaagc atatgaacaa ttgtcggcgc atttcgagcc aactccgaag cttggcccga
     4621 ttcttgttga agtacttggt ggtcagctac ttaacaccca agttagactg tggccacgct
     4681 tcttctttat tgttgccttt aaaatacaga tattcactag gtcagcaaca ttaatagaga
     4741 gagggtgcaa atagcacaaa gtataaatgc gcctacaaag aaccttctaa aagtcctaaa
     4801 acggctaaat aagaatttga aaataaaaaa taagacgaaa aatatgcaaa gtactcctgt
     4861 aaaaccagag acaagtttcc cataagtctg aactttcttt tttagtttac caatcaaagt
     4921 agaatctagg ttcacagaat ttttgctttg ataatactga atttctgcaa aataagccat
     4981 tggggaaagt gttttaagag cttcaagctt atctatatct cgaaagaaat ccacataatc
     5041 tcgcaaaagc aatattttgt aggtttgata ttcaacggct tgttggaaaa cttcatagca
     5101 tttcgtggaa agtgccttgg cgtctataat tggagcggga acatgtgctg gaaaccctgc
     5161 atgagtccaa atccaataat cttttaaagc tggacattgg ccactgagtg cgagctgtgt
     5221 ttccacctca ggaatatccc tctcttgatt gatcgaagtg atttgtttgt aggtgtctgg
     5281 cgaattgata gcatccagag cagacctata aagattctgc aaatctatca gaccactttg
     5341 tgaatcaata gcctcaaagt catttccgag atgatccaca cgtgttgctt ttcctagatc
     5401 aaaagaattg ccttggtaga cgagcccgac gatatcaaag tatttattaa agacggggct
     5461 accactctgg cctttttgaa ttgtaaaatt aagtccaaac tctgatgcag tggaattgga
     5521 gacatttccc atttgtatat ttaggaattc acagttagga aaaccaatgg tgaagatctc
     5581 ctcatcatcg acggggcttc taattttgcc agaaattcct tttatattga ggtttctcgt
     5641 cggctttaaa atggccagat cggtttttgg gaaggttgca atgatttgta gatccactaa
     5701 gccgatagaa acaggtgtag cccccagcgc tgaggctcgg gatatcttaa catttgggca
     5761 cagagattga ttgcattttt gagtaataac gtgagcatta gttataattt ttccagagga
     5821 taaaacaaaa ccggttccgc agccgtcatc gccatctaaa actataatgg attccttaag
     5881 agactcggcc gggacgttaa ttgcggcact agcgaaggcg taaaaactaa atattaatga
     5941 aaggaggtat ttcatacata gatgggataa aacaaaaaga caacgttatc aacgcatatc
     6001 tttgccgaga gagttgcaaa gattacctcg caagacaagt ggttttgggc tggacgagtg
     6061 ccccaattgt attggaaggc tggcaattct tagccaaaat gttacagacg ccaagtcctt
     6121 ttgaccttcg atattttttc ttcaaagtca tgaacgcgca ttgaataatc agctcccgca
     6181 aaatctaata caaaaccgag cgggaaaatt ccaaaaaaat ttcagcgatt ttttcaagaa
     6241 agctcattca gacggaacac gctttggcca gtaagacaaa ggacagaagc aaaaaaactg
     6301 aaaaactcac tgagttcgga agaaaatatt ttgccaaaac ctcttatatt cacgctgagt
     6361 tatctccgcc ggaacccaga gcgttggctt aaaagatata gggactgatc gagagtccga
     6421 tcgcagcaat actggatgag gattttctag gtttctgagt tttaccactt tttttctttt
     6481 atttcgagag aggattcttg ataggccagg gttggagtga gccgccgccg g
//
LOCUS       DATBTE010000078         8869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1374741, whole genome shotgun sequence.
ACCESSION   DATBTE010000078 DATBTE010000000
VERSION     DATBTE010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 8869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8869
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374741"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VIG33_03040"
     CDS             complement(<1..313)
                     /locus_tag="VIG33_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269835.1"
                     /translation="MTQKPACIDSKVVERIDRGSESVFRCGLNRHVPFSTYFTKHLSA
                     IEERVQQIEKFTEMIAPYKASLRLVILKNKPNYFQVSKNLIYVGEPLLETRGHLEKAL
                     LK"
     gene            complement(425..1360)
                     /locus_tag="VIG33_03045"
     CDS             complement(425..1360)
                     /locus_tag="VIG33_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEY8269836.1"
                     /translation="MTVKKVTIVEMGLRDGLQNEKVVLSTEDRLEILKRLLDAGSRRV
                     EVGAFVSPQWVPQMAGSAEVTLAALRMQKEKKSPKGTEFSVLVPNEKGMSMAIETGVK
                     EVAIFAAASESFSKKNINCTIEESFERFKVVLDLAKKHKIKVRGYLSTCFGCPFEGKI
                     SEAKVIRLAKRLHQIGCYEISIGDTIGIADPAQVERVFKKLKKVVPVKKLAAHFHDTR
                     GQALANILVTYNLGIRVFDASVGGLGGCPYAPGAAGNVATEDVAYMFQGMGVKTGLNI
                     EKMVAMNPWLTEKIQHTLPSKVGKVGLLKPLGKLG"
     gene            complement(1357..1755)
                     /locus_tag="VIG33_03050"
     CDS             complement(1357..1755)
                     /locus_tag="VIG33_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEY8269837.1"
                     /translation="MEIKIRHNGTEFKAEAQVIKETLWVHHKGRTFAVEAQPSSRKRD
                     KKGTGASSDKVLAPMPGKVTKILVGLNQQIDKGQAVLVMEAMKMEYTLKAEAAGKITQ
                     IECQSGDQVILGKMLVKIEAEIKDGSGSKI"
     gene            complement(1757..3232)
                     /locus_tag="VIG33_03055"
     CDS             complement(1757..3232)
                     /locus_tag="VIG33_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY8269838.1"
                     /translation="MRKFKRIAIANRGEVAVRIIKACEEMGIETVLLHSDVDINTRAY
                     RMATKTVCIGPAETAKSYLNIAANIEGALAGSAEAIHPGFGFLSENADFADACVKAGI
                     VFIGPSAQAIRSLGDKVHCKALAKKAGLPLVPGYEGEAQEVSRLMAEAESIGFPVIVK
                     AAAGGGGRGMKLITNMKEARERIESAQREALSAFGSAKVFIEKYLDHAKHIEFQIFGD
                     STGKVVHFFDRECSVQRRHQKIIEEATSPSLTDDLRRQMGEAAVAIAQLGKYKGAGTV
                     EFLLQDEKFYLLEVNTRLQVEHPVTEMVMGVDLVKAQILTAQGEFVFNSQQLRVPHGH
                     SIECRIYAENPYLGGVPSTGKLGKIEWPEGPGRRYEFGFDEGDEITPYYDPMIAKVIV
                     WDESRSRAIQKMVRVLKDSIVFGVHTNIPYLIEILTHAEFVNGTMTTRFIETYFKDPL
                     VAPELSEVEKKLVEKALQQARGGSGNSLRQESPWQSFWRGI"
     gene            complement(3232..4011)
                     /locus_tag="VIG33_03060"
     CDS             complement(3232..4011)
                     /locus_tag="VIG33_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8269839.1"
                     /translation="MSFVEVKEAAHVAYVKLYRPEIRNAFDPSMIAEITKAFSDLNQR
                     SDLRAVVLQGEGKVFCAGADLNWMKEMVGYTLEQNKEDSIKLFAMFEAIATCSLPVIG
                     LVQGAAFGGALGLVACCDYVIVDPQTQFCFSEVKLGIAPAVISSFVQRKCGIGTVRHL
                     MMSGTVFNSQQAENIGMVNEIASSTEFTARLASILKTYKECGPEAVRETKKLLNDLPH
                     LSWNDQRKRTSQLIAERRVSAEGQEGLMSFLEKRSPSWRNS"
     gene            complement(4014..5621)
                     /locus_tag="VIG33_03065"
     CDS             complement(4014..5621)
                     /locus_tag="VIG33_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY8269840.1"
                     /translation="MEVIESVVDSNSPDFKLNAEAMMGHVQMWRQRIEQIKLGGGEDA
                     LKKHKSRGKLTARERIEALLDSASAFLEFSTLAAWEMYENQAPAAGVITGIGTIHGTE
                     CVIVANDATVKGGTYFPMTVKKHLRAQEIAMENGLPCIYLVDSGGAFLPLQAQVFPDR
                     DHFGRIFYNQARMSAQNIPQISVVMGSCTAGGAYVPAMSDENVIVKDNGTIFLGGPPL
                     VRAATGEVVDAQELGGAKVHCESSGVTDHFAEDDQHAIEITRSIVAHLNHRRKVTMKT
                     LPIVEPLYDAKELYGVIPKDSRIPFDVREVIARIVDGSRLHEFKSLYGKTLVTGFAHI
                     WGMPVGIIANNGVLFSESSLKAAHFIELCEQREIPLIFLQNITGFMVGKKYETEGIAK
                     HGAKMVMAVSNARVPKFTVVIGGSYGAGNYGMCGRAFQPRQLWMWPNARISVMGGEQA
                     SNVLLTVKLDQMAAKGQTMSAEQQADFKRPTLEKYDHESSAYYSSARLWDDGIIDPTD
                     TRRVLALGIAASLNKEWGPQVQGVFRM"
     gene            complement(5631..6194)
                     /locus_tag="VIG33_03070"
     CDS             complement(5631..6194)
                     /locus_tag="VIG33_03070"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEY8269841.1"
                     /translation="MFSTFRKNIEKNLRDRGVSLRPTDLAALVEGVAPKSTRIALSYA
                     LDFLRPFSAGMGFRVTRLSDRHIELVIPARKRNEDEEGRIHEGALWTAALEAARLLWL
                     RHAPLGGFRVHLVSGNFHILRDYVGDVRVRMELPEMQCETVLAKIRSHQQAQTEVTLH
                     FYDEKEKSVAEATMTLQLKHVPELNAP"
     gene            6336..6806
                     /locus_tag="VIG33_03075"
     CDS             6336..6806
                     /locus_tag="VIG33_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269842.1"
                     /translation="MKKIISLIIFTAALFWTWNLVHSSAAIGFETHSGIQEKLAFLIK
                     QTVMEKKPSSKDFKIKRLWTESLGETKVRAVFAYEFKESSEGGGDTTEQTVEGEAILF
                     REPQDESKLDRWTLQSVRTTNDIITFNEGLVVTPGAEIPEIAPSEAPTTESEHQ"
     gene            6806..7855
                     /locus_tag="VIG33_03080"
     CDS             6806..7855
                     /locus_tag="VIG33_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8269843.1"
                     /translation="MELQLPERFVQIDEDGYALLGEVRTQAPQVGGEVLSEMSFAENG
                     AFMSRCGGVPVIVEAFDEPYVASQISLENKIWRMELPYNVRFEFELASLSLDEWDRFH
                     GKANNEIPFVMSRKAQAHFFNLLDSFDDNSITFAGKTYEIPSFFPSNKELAKSDYWTK
                     IYHQEVNPGWNLGEPAEALKDMLPRLKLPKSRVLVLGCGEGHDAALFAQAGHVVTAVD
                     FSEEAIARAEKNYGHLPGVRFLKQDIFKLDQSFQSSFDLVFEHTCYCAIEPRRRQELV
                     KVWKQVLHDQGQLMGVFFTMYKKQGPPYGGSEWELRQRLKDSFQFIFWGRWRQSRPNR
                     QGKELFVYAQKRSLK"
     gene            7966..8487
                     /locus_tag="VIG33_03085"
     CDS             7966..8487
                     /locus_tag="VIG33_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269844.1"
                     /translation="MKSLFFALVILSVTMSVGNAQTDSEERTEKEFYSLIENYLHSCS
                     GHCQAPFQSKMAFNIRQPERSILSEEVKSNLKRVASSQAQIWGDTILEGDYYSAGHTQ
                     LDSVLVLFKNEILIGYKITYSERAWFIGDCDFDSNDEETLKNCNEGHIHESTFVSPDF
                     NTFFSEDFADFAN"
     gene            8637..>8869
                     /locus_tag="VIG33_03090"
     CDS             8637..>8869
                     /locus_tag="VIG33_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269845.1"
                     /translation="MLRFLIGIVFFLNFTCPAFAGLKISPASHSPIRPNVKWLRKELK
                     KMGLSKGFVSESLKYYEPGSFEKVLTLNLLGFL"
BASE COUNT         2416 a         2216 c         1745 g         2492 t
ORIGIN      
        1 ccttcaaaag cgccttttcc aaatgccctc gagtctctaa aagaggttct cctacataaa
       61 ttaaattctt actgacttga aaatagttcg gtttgttttt taggataact aaacgcaaac
      121 ttgctttata gggcgcgatc atctctgtga acttttcaat ctgttgaact cgttcttcaa
      181 ttgccgacaa gtgctttgta aaataggtgc tgaagggcac atgacgattg agtccacagc
      241 ggaaaacaga ttcactaccc ctgtcaattc tttcaacaac tttggaatct atacatgccg
      301 gtttctgggt catgtagagt atcccaagag taagtacgca aaatgtggtg actaagacag
      361 gtaataccca acgctgattc ataaggtctg aaacaagtgc aagagccaga ccagggaaat
      421 ttacttaccc caacttaccc aatggtttca gaaggccgac cttacctact ttactaggta
      481 aagtgtgttg aattttttcg gtgagccagg ggttcattgc aaccatcttc tcaatattca
      541 ggcctgtctt tacacccatt ccctgaaaca tatacgcgac gtcttcagtg gcgacattgc
      601 ccgcggcacc gggagcatag gggcatccac ctaaaccgcc gacactggca tcaaagactc
      661 ggatccctag attataggtc accagaatat tggccaatgc ctgtcctcgt gtatcatgga
      721 aatgagccgc taacttttta accggaacca ctttttttaa ttttttaaaa actctctcaa
      781 cttgagccgg atcagcaata ccaatggtat ctccgatgga aatttcatag caaccgattt
      841 gatgaagccg ttttgccagt cgaatcactt tggcttctga aattttgccc tcaaaaggac
      901 aaccaaagca tgtgcttaaa tagccacgca ctttgatttt atgttttttt gccaaatcca
      961 atactacttt aaaacgctca aagctttctt caatcgtgca attaatattt tttttagaaa
     1021 aagattcact ggctgcggcg aagatcgcca cttctttaac cccggtttca atcgccatcg
     1081 acatgccttt ttcatttggg actagcaccg aaaattcagt ccccttaggg ctctttttct
     1141 ctttttgcat tcgcagtgcg gccagggtca cctctgccga acctgccatt tgcggaaccc
     1201 actgcggcga aacaaaggct ccaacttcga cacgacggga tcccgcatcc aaaagtcttt
     1261 tcaggatttc caaacgatct tcagtactca aaacaacttt ctcgttttga agaccatcgc
     1321 gaaggcccat ttctacgatt gtaactttct taacagtcat atcttgcttc cgcttccatc
     1381 ttttatttcg gcttcgattt tcacgagcat cttacctaaa attacctgat ctccactttg
     1441 acattcaatt tgcgtgatct ttccggcagc ctcagctttt aaagtatatt ccattttcat
     1501 ggcttccatc accaaaactg cttggccctt atcgatttgt tggtttaaac ccaccagaat
     1561 tttagttact tttcctggca ttggcgccaa gaccttatcc gatgaagctc ccgttccctt
     1621 tttatctcgt ttacgcgaac tgggctgagc ttccaccgca aaggtacggc ccttgtgatg
     1681 aacccaaagt gtttctttaa tgacttgagc ttcggcttta aattctgtgc cattatgtcg
     1741 aattttgatt tccattttaa atccctctcc agaaggactg ccaaggtgac tcttgtcgaa
     1801 gactgttgcc gcttccaccg cgagcctgct gcaatgcttt ctcaacaagt ttcttttcaa
     1861 cttcagaaag ttccggcgcg accagtggat ccttaaagta agtttcaata aaacgcgtag
     1921 tcatcgtgcc atttacaaat tctgcatgag ttaaaatttc aatcaggtaa ggaatattgg
     1981 tatggacccc aaaaacgatg gagtctttca aaacgcggac cattttttga atggcacgac
     2041 tccggctttc atcccaaaca atgacttttg cgatcatggg gtcatagtaa ggagtgatct
     2101 catcaccctc atcaaaacca aattcataac ggcgaccagg tccttcaggc cactcaatct
     2161 ttccaagttt ccccgtgctc gggacacctc caagataggg attctctgca tagatccgac
     2221 attcaatgga gtggccatga ggtacacgca attgttgaga gttaaacaca aactctccct
     2281 gggctgtcag aatctgtgct ttcaccaaat ctacgcccat caccatttct gtcacaggat
     2341 gctcaacctg caatcgagta ttcacctcga gaagataaaa cttttcatcc tgcaacagga
     2401 actcgacggt gcccgcccct ttgtatttac cgagctgcgc aatggccacg gcagcttccc
     2461 ccatttgacg gcgcaagtcg tccgtcaatg aaggagaggt tgcttcttca ataatttttt
     2521 gatgacgacg ttggacagag cactcacgat cgaagaaatg aactacttta cctgtggaat
     2581 ccccaaaaat ttgaaattca atgtgctttg catgatccag atatttttca atgaaaactt
     2641 ttgcagaacc aaaggcagat aaggcttcgc gctgggcgga ttcaatcctc tcacgagcct
     2701 ctttcatatt tgtaattaac ttcataccgc ggccgccacc gccagcggcg gccttcacga
     2761 tgacaggaaa accaatactt tccgcttccg ccatcaaacg agagacctct tgcgcttctc
     2821 cctcgtaccc tgggactaaa ggcaggcctg cttttttcgc caacgcttta caatgaactt
     2881 tatcgcccag cgatcgaatg gcttgagctg aaggtccaat aaagacaatc ccagccttca
     2941 cacaagcatc cgcaaaatcc gcattttctg aaagaaaacc aaatccaggg tgaatggctt
     3001 cagcgctacc agctaaagcc ccttcaatat ttgcggcaat atttaaataa ctcttcgcgg
     3061 tttccgcagg tccgatacac acggttttgg tcgccattct ataagcccgg gtattgatat
     3121 caacatcaga atgaagaagg acggtctcga ttcccatttc ctcacaggct ttgataatac
     3181 gaacagccac ttcgccacga ttggcaatgg caattctttt aaatttcctc attaagaatt
     3241 tctccagcta ggtgagcgct tctctaaaaa gctcatcagt ccttcttgac cttcagcact
     3301 aactctacgc tcagcaatca attgactggt gcgcttgcgt tggtcattcc aagaaagatg
     3361 gggtaaatca tttaataatt tctttgtttc acggacagct tcagggccac attctttgta
     3421 agttttcaaa atagacgcaa gccgtgccgt aaactcagtg cttgatgcga tctcatttac
     3481 catccctata ttctcggctt gctgggaatt gaacacggtg cccgacatca tcaaatgcct
     3541 caccgttcct atgccgcatt tacgctgcac gaatgagctg ataactgctg gagcaatccc
     3601 gagcttcact tcgctaaagc aaaattgtgt ctgcggatcg acgatcacat aatcgcaaca
     3661 ggccaccaag ccaagcgcgc cgccaaaggc cgcgccctgc acaagaccaa tcaccggaag
     3721 tgagcatgta gcaatggctt caaacatagc aaagagtttt atagaatctt ctttattttg
     3781 ctcgagtgta taacccacca tttctttcat ccaattcaaa tcagcacccg cacaaaaaac
     3841 tttgccttcg ccttgtaaaa caacagcgcg cagatcggag cgttgattca aatctgaaaa
     3901 ggctttagtg atctccgcga tcatacttgg atcaaaagcg ttccgaattt ccggacgata
     3961 gagttttaca taggcaacgt gagcagcttc tttcacttct acaaatgaca taattacatc
     4021 ctaaagacgc cctgaacttg agggccccat tctttattaa ggctcgcggc aattcccagt
     4081 gccaacaccc ggcgtgtatc cgtaggatca atgatgccat catcccaaag acgcgctgaa
     4141 gagtaataag ctgaactttc atgatcatac ttttccaaag tcggacgctt gaaatcagcc
     4201 tgttgctccg cagacatagt ttggccctta gccgccattt gatcgagttt cacagttaaa
     4261 agcacattcg aagcttgctc tccgcccatg acgctaatgc gggcatttgg ccacatccaa
     4321 agctgacgag gttgaaaagc acgtccacac ataccatagt tcccggcacc ataagagcca
     4381 ccaatcacca cagtaaattt aggaacacga gcattggaaa ccgccatcac catcttggcc
     4441 ccatgttttg caattccttc tgtttcatat tttttaccga ccataaagcc agtgatattt
     4501 tgcaagaaaa ttaaaggaat ctcacgctgc tcacacagtt cgatgaaatg agcggccttc
     4561 aaagaactct cactaaacag cacgccatta ttggcgataa tccccactgg cattccccaa
     4621 atgtgagcaa aaccagtcac cagagttttt ccatacaaag atttaaactc gtgcaagcga
     4681 gaaccatcca ctatacgagc gataacttca cgcacatcga atggaatacg agagtcttta
     4741 ggaataaccc cataaagttc cttcgcatcg tataatggtt caacaatcgg caaagtcttc
     4801 atcgtcactt tgcgacggtg attcaagtgc gcgacgatgg agcgagtgat ttcaatggca
     4861 tgttgatcat cttctgcaaa atgatctgtc acgccgctgc tttcacaatg aactttggct
     4921 ccacccagct cttgcgcatc gacaacttcg cccgttgcgg cccttaccag cggcggtccg
     4981 ccaagaaaga ttgtgccatt atcttttaca atgacattct cgtcactcat cgccggaaca
     5041 taagcgccac ccgcagtaca agaacccatg accacagaga tttgcggaat attttgtgcg
     5101 ctcattcgag cttgattata aaaaatacgg ccaaagtgat cgcgatcagg aaaaacttga
     5161 gcttgtaatg gcagaaaagc tccaccggaa tccaccagat aaatacacgg caggccgttc
     5221 tccatcgcga tctcttgagc ccgcaaatgt tttttcacgg tcatcgggaa ataagtgccc
     5281 cctttcacgg tggcgtcatt tgcaacaatc acgcattcgg tcccgtgaat agtaccaatc
     5341 cctgtaatca cccctgcggc gggagcttga ttctcataca tttcccaagc tgctaaagtt
     5401 gaaaattcca aaaaggcgct ggctgaatcc aaaagggctt caatacgttc acgggcggtt
     5461 aactttccac gagatttatg tttttttagc gcgtcttctc caccacctaa ttttatttgt
     5521 tcaatacgct ggcgccacat ttgtacatga cccatcatag cttcggcatt taatttgaaa
     5581 tcgggggagt tgctatccac aacgctttcg atcacttcca cgagaactcc ttaaggtgca
     5641 ttcaattcag gaacgtgttt taattgtaaa gtcatcgtag cttctgccac tgacttttct
     5701 ttttcgtcat aaaaatgcaa agtcacttca gtttgggcct gctgatggct ccgaatttta
     5761 gccaacacgg tctcgcattg catttcggga agctccattc gcacccgaac atctcccaca
     5821 taatcacgca atatatggaa attgccgcta accagatgaa ctctaaaacc ccctaaaggg
     5881 gcatgacgaa gccacaaaag tcttgcggcc tccaaggctg ccgtccagag agctccctca
     5941 tgaattctcc cctcttcatc ttcgttgcgt tttcgcgccg gaatcacaag ttcgatatgg
     6001 cgatctgaca aacgggtcac tcgaaagccc atccctgctg aaaagggccg caaaaaatcc
     6061 aaagcataac ttaaagctat acgagtggat ttaggagcga caccttcgac caatgcagct
     6121 aaatctgtag gacgaagact gactcctcgg tccctcaaat ttttctcgat atttttacga
     6181 aaggttgaaa acataaagct tccttttttc attcttctta aggttgaaaa gtaggaaata
     6241 tcatgctcct atgaacttgt ctaactttcg agtattactt ttaaattctg ctttcaagtg
     6301 ctatatttta aatctcaaac aggaattgag ggcctatgaa aaaaattata agtttgatca
     6361 ttttcaccgc tgcgctattt tggacttgga accttgttca tagctctgcc gctataggtt
     6421 tcgagacgca ttccggcatt caagagaaat tggcattttt aattaagcag acagtgatgg
     6481 aaaaaaaacc ttcctctaaa gattttaaaa ttaaacgact ttggactgaa tccctcggcg
     6541 aaactaaagt tcgcgctgtt tttgcttacg agttcaagga gtctagcgaa ggtggcggcg
     6601 acactactga gcaaacggtt gaaggtgagg ccattctttt ccgtgaaccc caagatgaat
     6661 ccaaactgga ccggtggact ttacaaagcg ttcgaaccac aaatgatatc attactttca
     6721 acgagggcct cgttgtaact ccaggagctg aaatccctga aatcgcaccg agcgaagctc
     6781 ccactactga gtctgagcat caataatgga gcttcagctt ccagaacgct ttgttcaaat
     6841 tgatgaggat ggctatgctc tcctgggaga agttcgaact caggctccgc aagtaggggg
     6901 agaagttctg tcagaaatga gctttgctga aaatggtgcc ttcatgagcc gctgtggcgg
     6961 agttccggtc attgtcgagg ccttcgatga gccttatgtc gcttcccaaa tttctctcga
     7021 aaataagatc tggcggatgg aacttcctta caatgtaagg tttgagttcg aactcgcctc
     7081 tctcagtttg gatgagtggg atcgcttcca tggcaaagcc aataatgaga ttccttttgt
     7141 catgtcgcgg aaagctcagg ctcatttctt taatctgctc gactctttcg acgataactc
     7201 catcactttt gcaggtaaaa cttatgagat tccatccttt tttccaagta acaaagaact
     7261 ggcaaaatca gattattgga ccaaaattta tcaccaagaa gtcaatcctg gttggaattt
     7321 aggtgaaccg gccgaagctt tgaaggacat gcttccccgc ttgaaattgc caaaatctcg
     7381 agttttggtc cttggctgcg gcgaaggaca cgacgccgcc ttgtttgcac aagcgggcca
     7441 tgtggtcacc gccgtggatt tctcagagga agctattgct cgcgctgaga aaaattatgg
     7501 ccaccttcct ggggttcgct tcttgaagca agatatcttt aaactggatc aaagttttca
     7561 aagctccttc gatctagtct tcgaacacac ctgctattgc gccatcgaac caagacgtcg
     7621 ccaagaactt gttaaagtct ggaagcaagt tctccacgac caaggccaac tcatgggcgt
     7681 attttttacc atgtacaaaa agcagggccc tccttacggc ggttctgagt gggaactccg
     7741 ccaacgcctc aaagattctt ttcaatttat attctggggt cgctggcggc aatctcgacc
     7801 gaaccgccaa ggcaaagagc tttttgtcta cgctcagaaa cgctccctta aatgacaaga
     7861 atttcaaaag ttttaatatg cattttatgt ataatatcat tatttacata aacttttaaa
     7921 agttcttttt ctcctttata gtttcctcct caggagaagt ctgccatgaa aagtttattc
     7981 tttgctctcg ttattttaag cgtaactatg tccgttggaa acgcacaaac agactctgaa
     8041 gaacgtactg aaaaagagtt ttacagtctc atcgaaaatt atctccactc ttgcagcggt
     8101 cattgccaag ctccttttca aagcaaaatg gctttcaata tccgacaacc agaacgatcc
     8161 attctgtccg aggaagtaaa atcaaatctt aagagggttg cttcttcaca ggctcaaatt
     8221 tggggggata cgattcttga gggcgactat tattctgccg gtcataccca gttggatagc
     8281 gtcttagttc tttttaaaaa tgaaatcctt atcggctata aaatcaccta ctccgaacgt
     8341 gcttggttta tcggtgactg tgattttgat agcaatgatg aagagactct aaaaaattgc
     8401 aatgaagggc atattcacga gtccaccttt gtttccccgg actttaacac ttttttcagc
     8461 gaagattttg cagatttcgc taactaaatt aaacttataa agtttatatc tcgaccaaag
     8521 tccttctcta tcgtttaacc tcgagacgta aggaccgttt cactcaatag tggcccactc
     8581 ttccctattt cgaaatatca taagtaaata tcttgctgcg ttttcaggga ggatctttgc
     8641 tgcgtttctt aataggtatt gtgttttttt tgaattttac ctgcccagca ttcgcaggcc
     8701 tcaaaatttc acccgcttcg cattccccta ttcgtcccaa tgttaaatgg ctgcgtaaag
     8761 agctgaaaaa aatgggtctc tccaaaggct ttgtctctga gtcccttaaa tactatgaac
     8821 ctggatcatt tgagaaagtc ctaaccctta atcttttagg atttttaca
//
LOCUS       DATBTE010000079         3295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1306188, whole genome shotgun sequence.
ACCESSION   DATBTE010000079 DATBTE010000000
VERSION     DATBTE010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306188"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..1876
                     /locus_tag="VIG33_03095"
     CDS             443..1876
                     /locus_tag="VIG33_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY8269846.1"
                     /translation="MNCKYAFLLSLVFCHLGASAMTLEEFLSEVRKRNKDFQALGTSQ
                     EVAEDRREAGDIGLSPVLTIAGGYLSDEKQPQTIGTKVVSHQYSVGIAKEFSSGTQVS
                     LSGNVQQIQISDIPQGSPSYLSNYALGSLGISLSQSLWKNAFGAGTRLRQKRQASAAE
                     LEKQTYNLQQRQILIDAETAYWNYLYQQDELQMRRESLERAKRIETWLRRRYRDGIND
                     KADYLNAQALTASRDLLLTTARDHALAAEQSIRVLLELDPKEKPPVLDSDFKKPRDLR
                     VLVGADLKGVSSGRVVRLDAYLASLEARTKSIGAQEVADALKPDLVLQAAYNTNSNIQ
                     NTTTQALNKINDLDIPTTQVGLKFTYVFDTDAKISQGDVSRKEALAAQLKSERKMIES
                     ESSWSELQRRYGEMLKQIEMADRISQVQSARAREQAIKLSRGRAVTSDVIISEEDAST
                     SLLTLNRLHVEARKMEAQSRLFVRLSE"
     gene            1902..>3295
                     /locus_tag="VIG33_03100"
     CDS             1902..>3295
                     /locus_tag="VIG33_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8269847.1"
                     /translation="MNLSALSIKRPIFISCIVALMLILGVICLGKMPVDLFPDVTFPI
                     ISVQVTYPGASPIDLEKQVSKPIEDELGSLSGLKNMTSNNLDSAAIIILEFKLGTDVK
                     EAEQQVRNRIGNIRRTLPNDIYEPVIRRFDPADQPVITLAITSNLPPGQAFDVASELI
                     KPQFERINDVGQVTVVGGRKTEIQVLVDKNKIQDRQISMMQIAKRIEETSKDIPIGKV
                     ENSSKELLMRTSGEFSSLEAIKNVNVNFMGSDRAVLLSDVGRVERGLEDQKNMATIKG
                     SPALLLQVFKQSGSNTVAVTDAVKKNLEKVNKYLEEKKIEAHVQVVRDMSRPIRLNVA
                     DVKESILIGIVLCVLVVFFFLGSGRSTFITGMALPNSLLGGFVVMYVMGFSINMMTLL
                     ALSLAVGLLIDDAIVVRENIFRHMEMGKDPKTAALEGTQEVAQAVVATTLVVIAVFGP
                     ISSLQGIIGQFFKQ"
BASE COUNT          877 a          652 c          862 g          904 t
ORIGIN      
        1 cacggtggcc tggcttggaa ggcccgcagg tttattcacg atgataagca cttcgtcctc
       61 gaagaggata ggaatcgttg ggggaaagtt tgcagtcttc gtattcataa atgtggcttt
      121 tcctggagca ttgattctat atgaggaatt tcagcgaagg gtcaaaaata agaattaaat
      181 taatagatgc ggcgctccgc aaaggtccag gttgagttct ctacgaatct gcaggaggag
      241 agttaagatt tatcaggatt caattaagat gagctcgagc ctgaattgag cttatatttc
      301 aacttctttg atttcaataa gggtttgaaa tatgatcggt aagtataatt gaagggagcg
      361 caatcgctct ggtttataag aagcaaactt atgatttgca gattcaacct cttacgattt
      421 ttgttcaagg gattctcgcg tgatgaattg taaatatgcc tttttgctga gtttagtttt
      481 ttgccatctc ggggcttccg cgatgacgct cgaagaattt ttatctgaag tgcggaaaag
      541 aaataaagac ttccaggcgc tgggcacttc gcaagaggtc gctgaagatc gcagagaagc
      601 tggcgatatc ggtttgtcgc cggtgctgac cattgccgga ggttacctta gtgatgaaaa
      661 acaaccgcaa accatcggca cgaaagtggt ttcccatcaa tacagtgtgg gtattgccaa
      721 agagttctcc agcggaaccc aggtttcttt gagtggcaat gttcaacaga tccagatcag
      781 tgacattccc cagggaagtc ccagttatct ctcaaattat gcgctcggga gtttggggat
      841 ttctctttct caatctctat ggaaaaatgc ttttggtgct ggaacacgtc tccgtcagaa
      901 acgccaggct tcagctgcgg aactggaaaa acaaacctat aatttacaac aacggcagat
      961 tttaatcgat gcagagactg cctattggaa ttatttgtat cagcaagatg aattgcaaat
     1021 gcgccgggag tccctggaaa gggccaaaag aatcgagact tggttgcggc gccgttatcg
     1081 agatggcatc aatgataagg cggactatct gaatgcccag gccttgacgg cttccaggga
     1141 tttgttgctg actacagctc gggatcatgc cttggccgcc gagcaaagta ttcgggtttt
     1201 attggaactg gatccaaaag aaaaaccgcc tgttttggat tcagatttta aaaagccccg
     1261 cgatcttcga gttctggtcg gcgctgatct gaagggagtc agttcaggtc gagtcgtgcg
     1321 cttggatgcc tatttggcct cccttgaggc gcggacgaaa agcatagggg cgcaagaggt
     1381 cgcggatgct ttaaaaccgg atttggtctt gcaagcggct tacaatacga attccaatat
     1441 tcaaaatacc acgacacagg ctctcaataa aatcaacgat ttggatatcc cgacgaccca
     1501 agtaggcctt aagtttacct atgtttttga tacggacgcc aagatctctc agggggatgt
     1561 gtctcgaaaa gaagcattgg ctgctcaatt aaagagtgaa agaaagatga ttgaaagtga
     1621 gagttcttgg tcagaactgc aacgtcgcta tggtgaaatg ctaaagcaaa tcgaaatggc
     1681 tgatcggatc agtcaagtcc aaagcgcgcg tgctcgagaa caggcgataa aattgagcag
     1741 ggggcgggcg gtgacttcgg atgtcatcat ctcggaggag gatgcttcaa cctctttact
     1801 gactctgaat cgtctgcacg tggaagctcg gaagatggaa gctcagagtc gcctttttgt
     1861 gcgactgagt gaataatgaa aataaataag cggagcgctt tatgaatttg tcagcattat
     1921 ctattaaaag acctattttc atttcttgta ttgttgcatt gatgttgatt ctgggagtga
     1981 tttgtttggg taagatgcct gtggatcttt ttcccgatgt gacctttcct atcatctcag
     2041 tgcaagtgac gtatcctgga gcttcgccga ttgatttgga aaaacaagtt tcaaaaccga
     2101 ttgaggacga gttggggagt ttgtcaggtc ttaaaaacat gacttcgaac aacctcgatt
     2161 ccgccgcgat catcattttg gaatttaagc tggggacgga cgttaaagaa gccgagcagc
     2221 aagtcagaaa tcgtattggc aacatccgta gaactcttcc gaatgatatt tatgagccag
     2281 tgattcgccg ttttgaccct gctgatcaac cggtcattac cttggcgatc acttcaaact
     2341 tgcctccggg gcaagctttt gatgttgcca gtgagttgat taaaccccag tttgaacgga
     2401 ttaatgacgt cgggcaggtg accgtcgtcg gtggtcgaaa aacagaaatc caggtgctgg
     2461 tggataaaaa taaaatccag gatcgtcaga tttccatgat gcaaattgcg aagcggatag
     2521 aagagacctc aaaagatatt cctattggta aagtggagaa ttcatcgaag gaacttttaa
     2581 tgagaaccag cggagaattc tcttctttag aggccattaa aaatgtcaac gtgaacttta
     2641 tgggctctga ccgagccgtt ctgttgagcg atgttggtcg cgtggagcgg ggccttgaag
     2701 atcaaaaaaa catggcaacc attaagggga gtcctgcact gttgttgcaa gtattcaagc
     2761 aatcgggctc caataccgtg gctgtgacgg atgccgtgaa aaagaacctt gaaaaagtga
     2821 ataagtatct tgaagagaaa aaaatagagg cccatgtgca agtggtgcgt gatatgtccc
     2881 gacccattcg cctcaatgtc gcggatgtga aagagtctat tttgatcggg attgttcttt
     2941 gcgtgctggt cgtgttcttt ttcttgggct cgggccgttc gacctttatt acgggaatgg
     3001 cacttccaaa ctctctttta ggaggctttg tggtgatgta tgttatgggc ttttccatta
     3061 acatgatgac cttgctggcc ttatctttgg cggtggggct tttgatcgat gatgccattg
     3121 tagtgcggga aaatattttc cggcatatgg aaatgggtaa agatcccaaa acagctgctc
     3181 tggaaggaac ccaggaagtg gcgcaggcgg tggtggccac cactctcgtg gttatcgcgg
     3241 tgtttggccc tatttcgtct cttcagggga tcattggaca gttttttaaa cagtt
//
LOCUS       DATBTE010000080         1655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_418290, whole genome shotgun sequence.
ACCESSION   DATBTE010000080 DATBTE010000000
VERSION     DATBTE010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418290"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            399..728
                     /locus_tag="VIG33_03105"
     CDS             399..728
                     /locus_tag="VIG33_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269848.1"
                     /translation="MKKIITTLLAVMSSISFAAADNAVTQDNINYIITVLDYSTWNRC
                     SKTVCAIAKAEGNGNGSCQKYPAIGVIATNLTTKGFDMLQSVSSCELVIEEDGEAFAQ
                     PRLGTSN"
     gene            728..1603
                     /locus_tag="VIG33_03110"
     CDS             728..1603
                     /locus_tag="VIG33_03110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HEY8269849.1"
                     /translation="MVKSWDDSFILNLLACQDYRAFMALFFQRSSDPKKRPKKLSFQR
                     FSTLAGYASKSYPAEILSGRKNLKYESIEKFAKGLSLNQTLSEYFKNLVLIDLTNSGV
                     TDFNIEDLKKRNDSIRIKIIAGIKDLRSSKKMNYSGLQKVLLQENFSQVFAALGSPQV
                     GASLKEIKNRTGLEIERLVKILSNLEKEKVIRRIENRYFLESNSIDFEFLESDIYFKR
                     NFVRSSTKALKCFDKQSRSASALYMTQTFSVHTEKLPALKNTLSSILREFSENAECPD
                     GDQVAEICLSFTTVK"
BASE COUNT          561 a          316 c          314 g          464 t
ORIGIN      
        1 ttttgaaaat aaaatcgaag gaaaccttta cgacggaaat caatttccat tgaatccggt
       61 cccttttgct atggcgttag gcattggtcc atgggaccgg taaaccggta aaaaaagccc
      121 catttaaaac cggtgcttcc acacaatttc cttttgtgca gaggttctct cactctaaaa
      181 tttaagaata ggacgaaata ctgatctctc tactgtgtta gtgcacagag aaaagaggcc
      241 attcagagat ggataggaat ggcaggatgg taaaaaatac caatcgttct ctacagagaa
      301 cgaaatatta ctcccttgct ttcaaaatat ccaaaggtgc agtagaagag atttcagaat
      361 ttcaggaaca aaaatattat tcacaaaaag gaatttaaat gaaaaaaatt ataactaccc
      421 tgcttgccgt aatgtcgtca atttcatttg cagccgctga caatgcagtc actcaagata
      481 acatcaatta tattataacc gttctagatt attctacgtg gaatagatgt tccaagacag
      541 tttgtgcgat agctaaggcc gaaggaaatg gtaacggtag ctgccaaaag tatcccgcaa
      601 ttggagtaat tgccaccaat ttgaccacta aaggttttga catgcttcaa agtgtttcta
      661 gttgtgaact tgtcattgaa gaagacggtg aggcgtttgc tcaaccccgt ttgggaactt
      721 caaattaatg gttaaatctt gggatgactc tttcatctta aatctactgg catgccagga
      781 ttacagggcc ttcatggccc ttttttttca aagaagctca gatccgaaaa aacgtccgaa
      841 aaaacttagc tttcaaaggt tttccacact agcaggatat gcttcaaaaa gttatcctgc
      901 tgaaattctc tccgggcgaa aaaatttgaa atacgagtct attgaaaagt ttgctaaagg
      961 cttatctctc aaccagaccc tttcagagta ttttaaaaat ctggttttaa ttgatttaac
     1021 aaattccggc gtcaccgatt ttaatattga agatctcaaa aaaaggaatg attcaataag
     1081 aataaaaata atcgcaggta taaaagatct tcgcagcagc aaaaaaatga attacagcgg
     1141 cctccaaaaa gtattactgc aagaaaactt ttcgcaagtt ttcgcagctc ttggaagccc
     1201 gcaggtcggc gcaagcctta aagaaataaa aaatcgaact ggcttagaaa tagagaggct
     1261 agttaaaatt ctttcgaatt tagaaaaaga aaaggtcata agaagaatag agaatcgtta
     1321 ctttctagaa tcaaactcaa ttgattttga atttttagag tccgatatct atttcaagag
     1381 gaattttgtg agatcaagta caaaagcctt gaagtgcttt gataaacaga gtcgaagtgc
     1441 ctccgccctt tatatgactc aaacattctc tgttcataca gaaaaacttc ctgctttaaa
     1501 aaacactctc tcctctatcc tccgagaatt ttctgaaaat gctgagtgtc cggatggtga
     1561 tcaagttgcc gagatttgtc tcagttttac tacggtcaag tgaagtgatt gccgcgagcg
     1621 ccgaaactaa accttcgaca ctcacgaaag aaaca
//
LOCUS       DATBTE010000081        11103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_760083, whole genome shotgun sequence.
ACCESSION   DATBTE010000081 DATBTE010000000
VERSION     DATBTE010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 11103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11103
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760083"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1160
                     /locus_tag="VIG33_03115"
     CDS             168..1160
                     /locus_tag="VIG33_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8269850.1"
                     /translation="MPFINLEQLSATPTAFSGPYKLEGSSTDDKFDLVKWRSDSLESK
                     SPPQKIHFFGTTEDPIALAKAGKTDTLDRDHVGANDQNELESRGWGISPTELTGETFI
                     ILNPKFLSEELRRYLYLKIDPAQIVVKIKEGQFKASYGLIPTGFPGELSLSDVEPLRK
                     EVPQYKGKKVSFKFDFDPGSEMEKQIAEYIKAAWSNDHIEVQLNPMSKGDKLQRMFEK
                     KSEAVLGRKGIDYPDGFSVLTYFKGKYESNYYFVDKPEIDASIAEAVKEFDSSKRATL
                     YKKIQLQILREHTLLPILFGSQASGMWSGKVQRAPSHPMGYHTMPFETIEMRTE"
     gene            1157..2944
                     /locus_tag="VIG33_03120"
     CDS             1157..2944
                     /locus_tag="VIG33_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8269851.1"
                     /translation="MKLAARLESQLTKLFLISAGVGAVVLFSSVSISTSEIKSKAIGF
                     ISTHVKAISQAEISSQNVSEIDREVRRLYEAWRGTQDIDIRIDIFLDGKLVGKAGQLQ
                     RFGVFSFSTNESHLLPSGQKLDVNIQLDLANQILTDVMMLAVFSVFLFSCFCLLRKNL
                     GKTIQQVTTPLERRVAWLTEVAQKLPESIQGELLMEDTAIDEVQALDVSLNAFAKQII
                     ALESRVKEAGIKEGRVRMAEQLAHTVKGKLSLLYLRIDNSIGLSEAEKHRLASAVSDV
                     ATAAQTLLKSGRLSHPDELSSAPGIANVNLTELLRRVIANRTETLPSEKQVSISFDFA
                     NEMDSVVCASAANLEATFANVIDNAVEAIDTDGKVRVKLRSENGFHKITVSDNGCGIP
                     EHILPQLMKERATFGKNDGNGLGLFHAKTTVEGLGGNIQITSTVGQKTEVTIALPVQN
                     SGSIKSEQGFEIDLSSVEQLVILDDDQSIHSAVMLLLGHAPSVEVVHLYSIPEFESWM
                     TENGSGEFNSRIYWMDYDLKHPSENGLTMIQKHSLQFEVYLVTGMAGSEEVQKIARAQ
                     NIRLFAKDEISKVQFKFNETFRLPEIGLG"
     gene            3048..4085
                     /locus_tag="VIG33_03125"
     CDS             3048..4085
                     /locus_tag="VIG33_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
                     /protein_id="HEY8269852.1"
                     /translation="MSDIDADKHNFGQLVKPMERASSLYFRKPRPVFWEHLFFGKESP
                     LESVFSEIGENGQVSLASLLFNLEIDVESEWLGYSKEIPRSNESVTDQHFYSFGVLLA
                     YAYLFGIRDLHKFNLIKTKTHLQVIDAEVVLTNLILPNESVLLPFKDVPFNVCGLNLL
                     VDSLVATISSQKQLIFAGYFDLFAVAFCKQRALLESLSVAASTAPIRIIIRNTKVYSN
                     HLNGSSKILDLLSEEQAQLERGDIPYFFKRLGDNQLYWISSTDGNTSSVQSTDSFTTD
                     VDRHAQQPSLLIGSPEVVEKKMAQGALFLQRKLGSSDSYEFSWNDGKLTLSPGEFKNT
                     FTGSQFRVGKS"
     gene            4188..4367
                     /locus_tag="VIG33_03130"
     CDS             4188..4367
                     /locus_tag="VIG33_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269853.1"
                     /translation="MENIIYRFFEIAFRAIGILLLTGVLVSALIDIQKRAFESKQTGL
                     TSMLKINEQLVGKIR"
     gene            4389..4679
                     /locus_tag="VIG33_03135"
     CDS             4389..4679
                     /locus_tag="VIG33_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269854.1"
                     /translation="MKNKKNDQENKDFFGDKWKLAPGGRKSIDKCQTRVWELYKELNG
                     YASLFRNMGNCELDCDELYGISLSLQRMGKRLGKISDKLSGAIVKEDTSDTI"
     gene            4968..5294
                     /locus_tag="VIG33_03140"
     CDS             4968..5294
                     /locus_tag="VIG33_03140"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEY8269855.1"
                     /translation="MPHLGHNPTLPDIQKYVDELEQERGFANQDAIQKCLMLGEEVGE
                     LFKAIRKQENMSVDTKSQSFSVQEEMADIIIMLCSIANRFDVNLEDAFRNKEEINKKR
                     KWTTIK"
     gene            5291..5947
                     /locus_tag="VIG33_03145"
     CDS             5291..5947
                     /locus_tag="VIG33_03145"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8269856.1"
                     /translation="MKHWVFDLDGTLVDSLTSHFQVLEKIFHRFDADFSSSEHLEVLK
                     VNSKTLPKYFETKLGVAAVEPALVMFKELNTQSFKSIRAFPGIEDLLKTLQTQGMKLA
                     VWTARDLEATNEILKHTGLSPYFSICVSGSCVDEGKPHPEGLKKIAAHFECGAESMVM
                     VGDFDSDMLGAQAFGIKAIRVFWHPAVQIKKCEIADWQFHQVSEFRNWLSEQTLNQEK
                     "
     gene            5957..6730
                     /locus_tag="VIG33_03150"
     CDS             5957..6730
                     /locus_tag="VIG33_03150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8269857.1"
                     /translation="MKNSTLEFYDNLAENYQLIARDWDSAVSSQGQVLHALIGKHFSN
                     ASQIKLLDCSCGIGTQAIGLALSGYQVHATDLSPKAVAQARQQADRLKAKLTFGVADF
                     RTLDRDVEGKFDVVISCDNSLPHILSDDEVKISLENILDKLNPGGLFLASIRDYDELL
                     KTKPSGMVPRRIQDEIGERIYTQAWTWAEGKNEYEVELFLMLKSDLGWNTHSYKTQYR
                     ALRRSELSKFMSESGFTNIAWIMPEASGYYQPVVIGTKT"
     gene            6767..7645
                     /locus_tag="VIG33_03155"
     CDS             6767..7645
                     /locus_tag="VIG33_03155"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY8269858.1"
                     /translation="MISLVPTSPQYAELWQQWRQEPNSIHYNPIVGSDLEGLRRRMQS
                     CSSDLSNLKAAEEFQFFIQWTGQLVGTVSLKNVSFMMSYGEIGYGVGEEYQGKGIGSE
                     GIRTFVSKIFSETSLRRLFAYVAEDNFASRRLLEKNGFEQEGICREHYIINGTPTNEV
                     LYGLLRQDWEKGNKFSSPEITKKYRGMIVEESLEDPTIINNISIQNVQITSPSRWHIY
                     TVSLSKEQIDQYQKFLKRGPWYMHFWRGDDIVAVFKDKTFHLSKNDKGTWLPAIAYGK
                     SIGIPEEQLDFLTEGK"
     gene            7645..8598
                     /locus_tag="VIG33_03160"
     CDS             7645..8598
                     /locus_tag="VIG33_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269859.1"
                     /translation="MNHSKSKEEIAKMVRAIFQTRYSLSQGPLHEELRAVKADFNARG
                     ILQSGAYAVKVGKMVGQALQNAVDDTIDEIESARREARRKDSSALWDTTKEILSEICD
                     TEVTRANNQAVDIVGQTSRDGTFASIAAQHFSQFGSNLRWRIQTKIASLKAKSEFIAL
                     KTPEDRIAHGVPDVAIMMWFPTAERDGQEACEKAQNKFHTIEEVVLNATEGRATVRKF
                     DDPNILPQDRISAAIEKWLEKAVLVICDLEGNRPNVFYEFGYARAVGTDVLATRPTGS
                     ETALHLGHWQMTEYSDLDELKTKLLPKLKNHLEKFDLTGAS"
     gene            8747..10981
                     /locus_tag="VIG33_03165"
     CDS             8747..10981
                     /locus_tag="VIG33_03165"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269860.1"
                     /translation="MRWDETWHRLLEWTNRQTSSERLSAIILTHEGYKDLDPSHPLGG
                     KDGGKDALCTRDGKKWVMAVYFPRGKQSLKDIKGKFLEDLPGVQKNGADGIVFVTNQE
                     LTLGERDDIKALAEEAHCEIYHLERITTLLDTPNMHAVRKQFLDIDFAASTTTSPDDD
                     WKLLEENSKNLMSRVPDMIGNKVRLARKNDVENIEAKIKETPIVVVLGDSGVGKSGLC
                     KSIVAARDEKYHSLWLKAKSLNEQDFNAIGIKIGISGALQNLLKQGKKPGLLVLDGLE
                     KLSTDAEFTNLGILLGILLPLETEWQVILTCQPEAYDRIQQGLASVNVFTANWGKVEL
                     TAPKKEELEEVWKAFPNLQTIKLQRELSTLLDNLKILDLLASKSATPGALDPAKSWVG
                     ESDIIDWFWERFVLTQAEGPKRSRFMQILAEKIADAMDDEIAEVEFEGSDLDPVDSLL
                     ADGLCVRSSSNLGFAHDLYGDWARQRLIVSKKNGVKAFIEQKIIFPQWHRAIRLYGQQ
                     LLEKNQTVDAWKSAVKSFEVADGKPSLTQDLFLDAIVFCAQPYAMLERSWPVLVEDGG
                     KYLRRLLSRFRHVATSPNPAMLKYAKEVGIVSPAKAASFQRIPFGPQWIPVLVFLHSH
                     KEEVITLSLGLTADISYMWLSMAPHGAPGRNEAAELAVMCAEEILAVEATVDFTIFED
                     EVDETSLKAAMTAVKEQPQRVENLVKFACRRSEPVGALKAVVDKSAEEAQKVVQKSPL
                     HAPV"
BASE COUNT         3256 a         2221 c         2622 g         3004 t
ORIGIN      
        1 atttaattct gaattgaggc cgacttccat ctcttcatgt taattccgtc gatactggtc
       61 cctcaacctc cggttatatc actggaccat gggaccggta aaccggagaa aatagcccct
      121 ttttaaaccg gcgcgtgcag cacatagccg tggccgattg tgcaatcatg ccattcatca
      181 atttagaaca gctttcagcc actccaaccg cattcagtgg tccctataaa cttgagggct
      241 catcaactga tgacaagttt gaccttgtaa aatggcgctc tgactcactt gaaagtaagt
      301 cgccgccgca gaaaatccat ttctttggta caacagagga cccaatcgct ctcgctaaag
      361 cgggaaagac tgacaccctc gaccgcgatc atgtaggggc aaacgatcaa aatgaacttg
      421 agtcaagggg atggggtata tcaccaactg agcttacagg agaaactttc ataattttga
      481 acccaaaatt tttgagtgaa gaattgcgtc gctatcttta tttgaaaatt gatccagccc
      541 aaattgttgt aaaaataaaa gagggtcaat ttaaggcatc atacggtttg ataccaactg
      601 ggtttccggg agagctatct ctgtctgatg tggaaccctt aaggaaagaa gttccgcaat
      661 acaagggaaa gaaagtttca ttcaaatttg attttgatcc tggttcagaa atggaaaagc
      721 agattgcaga gtacatcaaa gcggcatggt caaatgacca cattgaagtg cagctcaatc
      781 ccatgtcaaa aggcgataag cttcagcgaa tgtttgagaa aaaatccgag gccgtccttg
      841 gtcgaaaagg aatcgactat ccagacggct tttcggtctt gacctatttc aaaggaaaat
      901 acgaatcgaa ttactacttc gtggacaagc cagagatcga cgcctcaatt gctgaggctg
      961 tcaaagaatt tgattcgagc aagcgtgcga cactttacaa aaaaattcag cttcaaattt
     1021 tacgagagca cactctgctg cctatccttt ttggttcaca agcgtctggc atgtggagtg
     1081 gtaaggttca aagagcgcca tcgcacccga tggggtatca tacaatgcca ttcgaaacga
     1141 tcgagatgag gactgaatga aattagcggc acgactggaa tcacagttaa caaaactgtt
     1201 tctgatctcc gcaggagtcg gtgccgtcgt ccttttttca agcgtctcta tttctacttc
     1261 tgaaattaaa agcaaggcaa tcggctttat ttcgacccat gtaaaagcca tttctcaagc
     1321 cgaaatcagt tcacaaaatg tcagcgaaat agatcgtgaa gttcgccgcc tctatgaagc
     1381 atggagaggc acacaggata tcgacattcg aatcgatata tttcttgacg gaaagcttgt
     1441 cggcaaagcc ggtcagttgc agcgatttgg cgttttttct ttttccacaa atgaatcaca
     1501 tttacttcca tcaggtcaaa agctggatgt gaatattcag cttgatttgg caaatcagat
     1561 tttaaccgat gtcatgatgc ttgcggtgtt cagtgtcttc cttttttcgt gcttctgtct
     1621 tttaagaaag aatcttggta aaactataca acaagtcaca acgcctcttg aaaggcgcgt
     1681 tgcatggcta accgaggttg cgcaaaagtt gccagaatct atacaaggtg aacttttaat
     1741 ggaagatact gccattgatg aagttcaagc ccttgatgtt tcgctgaatg cttttgccaa
     1801 gcagatcatt gcgcttgaat ctcgtgttaa agaggctggt atcaaagaag gtcgcgtgcg
     1861 aatggccgag caacttgctc ataccgtcaa aggcaaactt tcgcttcttt atctgagaat
     1921 cgacaactca ataggtctgt ctgaagcaga aaagcatcga ctagcttcag cggtgagtga
     1981 tgttgcaacg gcagcgcaga cacttttaaa atcaggtaga ttgagtcacc ccgacgagtt
     2041 gtcatcggcc cctggcatcg caaatgtgaa tcttacggag cttttacgac gggttattgc
     2101 gaatagaacg gagactttgc cttcagaaaa acaggtttca ataagttttg actttgctaa
     2161 tgaaatggat tcagttgttt gcgcctctgc cgcaaacctc gaggcaacat tcgctaacgt
     2221 catcgacaac gccgttgagg cgatcgacac tgacggcaaa gtgcgcgtga aactcagaag
     2281 tgaaaatggg tttcataaga ttacggtttc tgataatggc tgtgggattc cagagcacat
     2341 tttgccgcaa ctcatgaaag agcgcgcgac ttttggaaaa aatgatggaa atggcctcgg
     2401 actctttcat gcaaaaacaa ctgtagaggg tttgggcggg aacattcaaa tcacatctac
     2461 tgttggtcaa aaaaccgaag taacgattgc gttgccggtt caaaattcag ggtccatcaa
     2521 gagtgaacag ggcttcgaga ttgatctctc gtctgtagaa cagcttgtaa ttcttgatga
     2581 cgatcaaagt attcactctg ctgtgatgtt actgttaggg catgctccgt ctgtggaggt
     2641 ggttcacctg tattcgatcc cagagtttga gtcctggatg accgaaaacg gatcaggcga
     2701 atttaactcg agaatttact ggatggacta tgatttgaaa catccatcag aaaacgggct
     2761 caccatgatt caaaagcata gtcttcaatt tgaagtttat cttgtaaccg gcatggcggg
     2821 ttcagaagag gttcaaaaaa ttgcaagagc acagaacatc cggcttttcg caaaggacga
     2881 aatttctaaa gttcaattta agtttaatga aactttccgg cttccagaaa ttggtctggg
     2941 atgaggaaac taacagtggg aggaattatg aaagaagtag tcgaaatcaa tctcgatgct
     3001 cttgaagccg aagctgagtc attcagcgga ggcgtgagct cttgctaatg tctgacattg
     3061 atgctgacaa acataatttt ggtcagcttg tgaagcccat ggagcgggct tcatcacttt
     3121 atttccgaaa accgaggcca gttttttggg agcatctctt ttttggtaaa gagagtcctc
     3181 tagagtctgt tttttcggag attggtgaaa acggccaggt cagccttgca agcttgcttt
     3241 tcaaccttga gattgatgtg gagtctgaat ggctgggata ctcaaaagaa attccccgat
     3301 ccaatgagtc agtcactgat cagcatttct attcttttgg cgtgctgctt gcgtatgcct
     3361 acttgtttgg aattcgtgat ctacacaaat tcaatctcat caaaacaaag actcatctgc
     3421 aggttatcga tgctgaagta gtcttaacca atttgatttt gccaaatgag tcagttcttc
     3481 tgccatttaa agacgttcct tttaatgtgt gtggactaaa tttacttgtc gattcattag
     3541 tagctacgat ctcttcgcag aaacaattga tattcgccgg atactttgat cttttcgccg
     3601 tggctttttg taaacagcga gcattgcttg aaagtctaag cgtcgccgca agtacagctc
     3661 cgattcggat catcattcgt aataccaaag tttactcaaa tcatctaaat ggtagttcca
     3721 aaatccttga ccttctttcg gaagaacagg ctcagcttga gcgtggtgat atcccgtatt
     3781 ttttcaaacg tcttggggac aatcagcttt attggatttc atcgacagat ggcaatacgt
     3841 cgtcagttca atccacagat tcctttacga ctgacgttga taggcatgcg caacaaccat
     3901 cgcttctgat cgggagtccg gaggttgttg aaaagaaaat ggctcagggg gctctctttc
     3961 ttcagagaaa acttggcagc tcagattctt acgaattttc gtggaacgat ggaaagctta
     4021 cgttgagccc aggcgaattt aaaaacacct ttacgggcag tcaattccgc gtaggcaagt
     4081 cttaaggctt taaaacaagg ataaaaacag ataactcggc gatgcaaatg ttgagactgg
     4141 acttctccgc gtaaggaagc tttccttggc gaaaagagga ggcagtcatg gagaatatta
     4201 tctatcgatt ctttgaaatc gcatttcgag caatcggaat tttactactt actggtgttt
     4261 tggtgtccgc gctgatcgac attcaaaagc gcgcatttga aagcaaacaa accggcctga
     4321 ccagcatgct aaaaataaat gagcagcttg ttggcaaaat acggtaatga ccggcgaggg
     4381 cttcggttgt gaaaaataag aaaaatgatc aggaaaacaa agattttttt ggagataagt
     4441 ggaaacttgc tcctggcgga cgaaagagta tcgataagtg ccagactcgt gtttgggagt
     4501 tgtacaaaga actcaacggt tatgcctccc tcttccgcaa tatgggaaat tgcgagctcg
     4561 attgtgatga gctgtacggc attagcctca gtctacagcg tatgggtaaa cgtctcggaa
     4621 agatctcaga caaactctcg ggcgctattg tgaaagagga cacgtcagat acgatttagg
     4681 cctacttttc cacttctgtg catagcgccg ttaagcttcg ttcttgctgg caatcgctct
     4741 atgcattata cctccgaggg taccacaatt taagtattgg agctccgagg actattgtcc
     4801 gattcggcca tccctcattt tccatgccgt tgtctcgaga ttccatttta agggtttgca
     4861 atggcgcata caaaaccaca attgatagaa aatttagcca ctgagcgact aaaaatcatg
     4921 gcaagtaaaa gagaactttt aaaacgactt tgccgaagga ataacctatg cctcatctag
     4981 gccataatcc cactttgcca gatattcaga agtatgtcga cgaacttgaa caggagcgtg
     5041 gattcgcaaa tcaagacgcc atccaaaagt gcctgatgct cggtgaagaa gtcggggaac
     5101 tttttaaggc gattcgcaaa caagaaaata tgagtgtgga taccaaatcc caaagtttct
     5161 cagtccagga agaaatggcg gacatcatca ttatgctttg ctcgattgcg aaccgatttg
     5221 acgtgaatct cgaggacgca tttcgaaata aagaagaaat taacaaaaaa agaaaatgga
     5281 caacgatcaa atgaaacatt gggtttttga tttagatgga accttagttg actcgttaac
     5341 gagtcatttt caagttttag aaaaaatctt tcatagattt gatgcggact tctcatcctc
     5401 tgagcatctt gaagtgctaa aagttaattc taagactttg ccaaaatatt ttgaaacgaa
     5461 gcttggggtc gctgctgtcg agcctgcgct ggtaatgttt aaagaattga atactcagtc
     5521 tttcaagagc attcgggcct tccccggtat tgaagactta ctgaagacct tgcagaccca
     5581 aggaatgaaa ctcgcagttt ggaccgctcg cgatcttgag gcgacgaatg agattcttaa
     5641 acacacgggg ttaagtcctt atttttcaat ttgcgttagc ggctcatgcg ttgatgaggg
     5701 aaagcctcat cccgaaggac taaaaaaaat tgctgctcac tttgagtgtg gtgctgaatc
     5761 aatggtgatg gtgggagatt ttgatagcga tatgctgggt gcgcaggcat tcggtattaa
     5821 agcgattcgg gttttctggc atccagcagt tcagattaaa aaatgtgaga tcgcggattg
     5881 gcagtttcat caggtatctg agtttaggaa ttggctcagt gagcaaactt tgaaccaaga
     5941 gaaataggga ttatttatga agaattcgac ccttgagttt tacgacaatc ttgctgaaaa
     6001 ttatcagtta atcgctagag attgggattc ggcagttagt tctcaaggcc aagtacttca
     6061 tgcccttatt ggaaaacatt tttcgaatgc ctctcagatc aagttgttgg actgctcttg
     6121 tggaatcggt actcaggcaa ttggcttagc attgagtgga tatcaagtcc atgccacgga
     6181 cctcagccca aaagctgtcg cgcaggctcg tcaacaggcc gacagattaa aagcgaaact
     6241 gacttttggc gttgcagact ttcgcaccct tgatcgcgat gttgaaggta aattcgacgt
     6301 agtgatctct tgtgataatt cattgccgca cattctgagc gatgatgaag tgaagatctc
     6361 cttagaaaat attctcgata aactaaaccc aggtggactt tttctagcgt cgattcgtga
     6421 ctacgacgaa cttcttaaga ccaagccaag tggaatggtc cctcggagaa ttcaagatga
     6481 aataggtgaa cggatatata cccaggcatg gacttgggct gaaggtaaaa acgaatatga
     6541 agtagaactc ttcctcatgt taaaatcgga tttaggttgg aacactcact cttacaagac
     6601 ccaataccga gcattgagga gaagtgaatt atcaaaattt atgtctgaat ctggttttac
     6661 taatattgcg tggatcatgc cagaagcatc tggatattac cagccagtgg ttattgggac
     6721 gaaaacgtga gacaaacttt tttcacctct tacgggatca catcaaatga ttagtctagt
     6781 gcccacttca ccacaatatg cggaactttg gcaacaatgg cggcaggaac ccaactccat
     6841 tcactataat cccattgtcg gcagcgatct tgaaggcctc cgccgtcgta tgcagtcttg
     6901 cagctctgat ctttcaaatt tgaaagcggc agaagaattt caattcttta ttcagtggac
     6961 aggtcaactc gtcggaacag tcagcttaaa gaacgtcagt tttatgatga gttatggtga
     7021 aattggctat ggcgttggag aggaatatca gggcaagggc atcggctctg agggcattcg
     7081 aactttcgtt tccaaaatat ttagcgaaac ttcattgcgg cggctatttg cctatgtagc
     7141 tgaggacaat ttcgcttcac gacgtcttct cgaaaaaaat ggtttcgagc aagaaggtat
     7201 ctgtcgtgag cattatatca tcaatggtac acccaccaat gaagttctct atggcctgct
     7261 tcgccaggat tgggaaaaag gaaataagtt ttcctcgccg gaaattacaa aaaaatatcg
     7321 aggtatgatc gttgaggaga gcctcgaaga tccaacgatc attaataaca tttcgatcca
     7381 aaatgttcag attacaagtc caagccgatg gcacatatat acagtctctc tttctaaaga
     7441 acagattgat caataccaaa agtttctcaa gaggggcccc tggtatatgc acttctggcg
     7501 tggtgatgac attgtggctg tcttcaaaga taaaaccttt cacctttcta agaatgataa
     7561 gggaacgtgg ctgcccgcaa ttgcatatgg caagtcgata ggcattccag aagaacaact
     7621 tgattttctt acagagggaa agtgatgaat cattctaaaa gtaaggaaga gattgcaaaa
     7681 atggttcgcg ctatttttca gactcggtat tcactttccc aaggccctct tcatgaggaa
     7741 ctccgcgcag taaaggctga ctttaatgca agaggaatac ttcaatccgg agcgtatgca
     7801 gtaaaagtgg gaaagatggt tggacaggca ttacagaatg cggtcgacga cacaatagat
     7861 gaaattgaga gtgctcgcag ggaagcacga agaaaagata gctccgcact atgggacaca
     7921 actaaagaga tattgtctga aatttgtgac acagaagtga cacgagcgaa taatcaagcg
     7981 gtcgatattg tgggtcagac atctagagac ggaacctttg catctattgc cgctcagcac
     8041 ttttcgcagt ttggaagcaa cctcagatgg cgcatccaga ccaagatcgc gagtctcaag
     8101 gcaaagtccg agttcatcgc acttaagact ccagaagata gaattgctca tggagttcct
     8161 gacgtcgcga ttatgatgtg gtttccgacg gctgaaagag atgggcaaga agcttgtgag
     8221 aaagcacaaa ataagtttca tacaattgaa gaggtcgttc tcaatgcaac cgaaggtcgc
     8281 gccactgtta gaaagtttga cgatcccaac attctccctc aggacagaat atctgcagct
     8341 attgagaaat ggcttgagaa ggccgtactt gtaatctgtg atctcgaggg aaatcgtcct
     8401 aacgtatttt atgaatttgg ttatgcaaga gcggtcggca ccgacgtgtt ggcgactcgg
     8461 cctacaggaa gcgaaactgc tctacatctc ggacattggc agatgacgga gtattctgac
     8521 ctcgacgagc tgaaaacgaa attacttcct aaacttaaga atcatcttga aaaatttgat
     8581 cttactgggg cttcttaatg cgagaagata gagactttct agccactcga ttgaatcgga
     8641 aattacaaaa aaatttattc aaacctacct taagccagga attcagattg cggatattgg
     8701 atgtggagca ggtcactact cttgattcat ttttaggtgg ttttatatga gatgggatga
     8761 gacttggcat cggcttctag aatggacaaa taggcaaact tcttccgagc gactttcggc
     8821 aataatactt acgcacgaag gatataaaga cttagaccca agtcatccgc tcgggggaaa
     8881 agacggtggc aaagatgcgc tgtgcacccg cgacggaaag aagtgggtaa tggctgtcta
     8941 ttttccacgt ggtaagcaat ccctaaaaga tatcaagggc aaatttttag aagaccttcc
     9001 tggagttcaa aaaaatgggg ccgatggaat agtttttgtt actaatcaag aacttactct
     9061 tggggagcgt gatgatatta aagctctggc tgaagaagct cattgtgaaa tataccatct
     9121 cgaaagaata acaactctgc tcgatacccc gaatatgcat gcagtccgaa aacaattttt
     9181 ggatatagat tttgcagcaa gtacaacgac atctccggat gatgattgga agcttctaga
     9241 agaaaactct aaaaacctta tgagcagagt tccggacatg atcggaaata aggttcgtct
     9301 agcaagaaaa aatgatgttg aaaatattga agctaaaata aaagaaactc caattgttgt
     9361 cgtgttaggt gattcgggag tcggtaaatc cggtctgtgt aaaagtattg ttgctgctcg
     9421 agatgagaaa taccatagcc tttggttaaa agcgaaaagt ttgaatgaac aagacttcaa
     9481 cgcaatcggg ataaagatcg gcatatcagg tgcccttcag aatcttctta aacagggtaa
     9541 aaagccgggg ctgttggtat tggacggtct tgaaaaacta tctacagacg ctgagttcac
     9601 taatctcgga attctcttag ggattttatt gccgcttgaa actgagtggc aggtgatttt
     9661 gacttgtcag ccagaggctt atgatagaat tcagcaaggt cttgcatccg taaatgtttt
     9721 taccgctaat tggggaaagg ttgaacttac cgctcctaag aaggaagagc tggaagaagt
     9781 ttggaaagcg tttccgaacc tacagacaat aaaacttcag cgtgaattgt cgactctttt
     9841 agacaatcta aagattctgg atttacttgc ctctaagtct gcaactccgg gagcccttga
     9901 ccctgcgaaa tcttgggttg gagagtctga tataattgat tggttttggg aaagatttgt
     9961 acttacccaa gcagaaggtc ctaagcgctc tcgattcatg caaattttgg cagagaaaat
    10021 tgcagatgcg atggatgacg aaattgcgga agttgaattt gaagggtctg atcttgaccc
    10081 agtggatagc ttgttagccg acggcctttg cgtaagaagt agttcaaact tagggtttgc
    10141 acatgatctt tatggtgatt gggcgagaca acgtctgatt gtaagtaaaa aaaatggtgt
    10201 taaagcattt attgaacaaa agattatttt tccacagtgg catcgagcta tcagattata
    10261 tggacagcaa ctattggaaa aaaatcagac agttgatgca tggaagtctg cagtaaagag
    10321 ttttgaagta gcggacggta aaccgagcct tacccaagat cttttcctgg atgcgattgt
    10381 attttgtgcg cagccttatg cgatgctgga gcgttcttgg ccagttctcg tggaagatgg
    10441 gggaaaatat cttcgaaggc tactcagtag atttcggcat gtagctacgt ctcctaatcc
    10501 cgcaatgttg aaatacgcaa aagaagttgg gatagtgtca ccggctaagg cggccagctt
    10561 tcaacgcatt cctttcggtc cgcagtggat tccggtattg gtttttcttc attcgcacaa
    10621 ggaagaagtc attacattga gtttgggatt gaccgcagat atttcatata tgtggttgag
    10681 tatggccccg catggagctc caggtagaaa cgaagctgcg gagttagcag taatgtgcgc
    10741 tgaggaaata cttgcggtgg aggccacagt cgacttcaca atttttgagg atgaagtaga
    10801 tgagacttcc ttgaaagcag ccatgacggc agtcaaggag cagccacaaa gagtagaaaa
    10861 tttagttaaa tttgcttgta ggcgttctga gcctgttgga gctcttaaag ctgtagtcga
    10921 taagtccgct gaagaagcac aaaaggttgt tcagaagtct ccgctgcacg cgccggttta
    10981 aaaaggggct attttctccg gtttaccggt cccatggtcc agtgatataa ccggaggttg
    11041 agggaccagt atcgacggaa ttaacatgaa gagatggaag tcggcctcaa ttcagaatta
    11101 aat
//
LOCUS       DATBTE010000082         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1170180, whole genome shotgun sequence.
ACCESSION   DATBTE010000082 DATBTE010000000
VERSION     DATBTE010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170180"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="VIG33_03170"
     CDS             <1..1364
                     /locus_tag="VIG33_03170"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8269861.1"
                     /translation="LEFMLNLLAGLRLCGITKDSKKASWGAYDVSVAESGPDAIKIIK
                     HAKKDFALILMDYHMPGMLGTEVTREIRQIKPSQQILAFSLDDTRDVMRENFLAGVND
                     FLDKNSDNDVLLAEIKNRCAKYDQLYRTIDSEDLPVDDRTLYILETGMIGRSMDLYQL
                     CKDIRKVAPSAATTVILGESGTGKELVAKALHKFSGRPKDRFVAINIAAESPLLLDSS
                     LFGHQKGAFTGATCNQVGKFELADKGTLFLDEIGDMPLDLQVKLLRVLQEKQITPIGS
                     NRPRSVDVRIVAATHKDLKKMVEAGTFREDLYYRLFNITLNTTPLRQRPEDIELLVAK
                     FTDEVCENNRIKRSLHRECLEIFRGYHWAGNVRELLSTVERLLLCADSSVVRKESLDP
                     VFFNPVKSKPITLEEIDAHVDSIKKHHIIDALKNSPTRAEAARRLNIAQNGLQYFINK
                     WNL"
     gene            complement(1550..>2414)
                     /locus_tag="VIG33_03175"
     CDS             complement(1550..>2414)
                     /locus_tag="VIG33_03175"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269862.1"
                     /translation="SAVLKNVSWETDSFGFRNHPIGELSKVDIIVGGDSCAVGTSLSQ
                     DETLSEILSRKTGKVVYNLGGVKADKIPDYLSALKLKPRFFVFVIIERSLGELPDDFN
                     SKKNSQFQGEKLAKLYQRAQDNITFNPFQSYKSKHGIFDSLLRIFIPLTDQDIAPGPY
                     EFKNGFLQGAQVEKIDRDQVVRSAVRRLAVYRKFFADQGIRFVVVGVPNKETIYFDQV
                     PLKHQPLILPMLQAELRSQGIEYIDLFQNFNSKYKESGALFHHPDDTHWNEAGVSIAA
                     DLLYETIKIQL"
BASE COUNT          740 a          512 c          493 g          669 t
ORIGIN      
        1 aacttgaatt catgctgaac ctccttgctg ggctcagact atgtggaatc acgaaggaca
       61 gcaaaaaggc ttcatggggc gcttacgatg tttccgtcgc agaaagcggt cctgatgcga
      121 ttaagattat aaaacatgcc aagaaggact ttgcattgat ccttatggac taccatatgc
      181 ctggcatgtt gggcaccgaa gtcacgagag agataagaca aattaagccg agccaacaaa
      241 ttcttgcctt ttcattagat gataccagag atgtaatgag agaaaacttc ttagcaggag
      301 taaatgattt tcttgataaa aattcagata atgatgtttt actagctgag attaaaaatc
      361 gttgtgcaaa atatgaccaa ctatacagaa cgattgattc cgaagatctt cctgttgatg
      421 atcgaactct atatatcctt gagactggaa tgataggtag atctatggat ctatatcaac
      481 tttgcaaaga cataaggaaa gtggcaccct ctgcggccac aactgtcatc ttaggtgaga
      541 gtggtacggg taaggaactt gtagcaaaag ccctccataa attcagcgga cgtcccaaag
      601 atcgttttgt agctatcaac atcgcggcag agtctccact ccttttagac tcctcccttt
      661 tcggccacca aaaaggagcc tttacaggag ccacatgcaa tcaagttggg aagttcgagc
      721 ttgccgacaa ggggactctt tttttggacg agatcggtga catgccattg gatcttcaag
      781 tgaaactact tcgagttctc caagaaaaac agataacacc gattggctca aacaggccga
      841 gatctgtcga tgtaagaatt gtcgcagcta cgcataaaga tctaaaaaaa atggtagaag
      901 ctgggacatt tcgtgaggac ctctattatc gactatttaa cattacctta aatacgacac
      961 cacttagaca gcgacctgaa gatattgaac tattagtcgc aaaattcacg gatgaagttt
     1021 gcgaaaataa ccgaatcaaa cgttcgctac atcgagaatg tcttgaaata ttccgaggct
     1081 accattgggc aggaaatgtc cgtgaacttc tgtcgaccgt agaaaggctt ctcctgtgtg
     1141 cggattccag cgtagttcga aaagaaagtc ttgatcctgt attttttaat cctgtgaaaa
     1201 gtaaacccat aactctcgaa gagatcgatg ctcatgtgga cagtatcaaa aagcatcata
     1261 ttatcgacgc tctgaaaaac tcaccaactc gcgccgaagc tgctcgacgt ttaaatattg
     1321 cccaaaatgg tcttcaatat tttattaata aatggaatct ctgatttttg ttacagaagc
     1381 gatactttta aatcaaattg gtcactatct ctttagtttc gacaaacgtt tagtgagcag
     1441 caaataaaga acccaggatt ttatcaacct gaattcgcct gatatagaag taacaataca
     1501 aataaccaaa atcgacgttc tactttttta tttttcgcgc aaaagtctct tacaattgaa
     1561 tcttgatcgt ctcataaagt aaatctgccg caatcgagac tcctgcttcg ttccaatgag
     1621 tgtcgtcggg atgatgaaat aaagctcctg attctttgta tttggaattg aaattttgaa
     1681 aaagatctat gtactcaatt ccctgggaac gaagctccgc ctgaagcatg ggtaaaatca
     1741 aaggctggtg tttcaacggc acctgatcaa aatagatcgt ttccttattg ggaactccta
     1801 ctaccacaaa acggattcct tggtccgcga aaaattttcg atagacggca agtcgccgaa
     1861 ccgcagacct aaccacttga tcccgatcta ttttttcaac ttgcgccccc tgaaggaagc
     1921 cattcttaaa ctcatagggt cctggtgcga tatcctgatc ggtaagcgga ataaatatac
     1981 gaagtaaaga gtcaaatatt ccatgttttg atttatagct ttgaaatgga ttaaatgtga
     2041 tgttgtcctg cgccctttga tagagttttg ccagtttctc accttggaac tgagaatttt
     2101 ttttgctatt gaaatcatcc ggaagttcgc ccaaggaacg ctcaatgatt acaaagacaa
     2161 aaaaacgagg tttcaatttg agtgccgaca ggtagtcggg tattttatcc gccttaactc
     2221 ccccgaggtt gtaaacgacc tttcctgtct ttcgtgaaag aatttctgaa agcgtttcgt
     2281 cttgcgaaag cgaagtcccg acagcgcaag aatccccgcc gacaataata tcaactttgc
     2341 tgagctcacc aatcgggtga ttacgaaacc caaaactgtc ggtttcccag cttacatttt
     2401 tcagaacagc actg
//
LOCUS       DATBTE010000083        12314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_213842, whole genome shotgun sequence.
ACCESSION   DATBTE010000083 DATBTE010000000
VERSION     DATBTE010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 12314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12314
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213842"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VIG33_03180"
     CDS             <1..1307
                     /locus_tag="VIG33_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269863.1"
                     /translation="IYFLLVIFLIGCGNVRFTASTSPEGSGSPTGVATTGNNDPNTGD
                     DGNTPGPTPTPPPPPNGPRDVHYSKAVPAASNQVDFLLVIDNSASMIAIQQKMAASMT
                     ALAKQLNGLAIDWQMCLTLTTDMNKGGVATNSGTHTCGSDGYCTGSYTGGSWSWGVSR
                     PWSSDYTPTGGASQYILKKTTNEALLNKIFTNTIPKLGSGDTGTGDERGIKAAYNHFA
                     NSANNGAFNTAGCYRPGSSVAVIVLSDEDERSVAGDCSRVNTALGDHEIPACRYYEAP
                     HNIRTLEFADQPANLLSQAKNIFGSSSRFTFNSIIADTDACQNQLNDNISFQVNGVSY
                     YSPHYTGTVYKAASQLTNGGVASLCSSDLQLNLFGDVVVNTLAKVTLECAPANLSIQV
                     GANEANVSSLDPSKYSVSGAVVTFNPDLTQNQWLKMDYQCTQ"
     gene            complement(1333..2532)
                     /locus_tag="VIG33_03185"
     CDS             complement(1333..2532)
                     /locus_tag="VIG33_03185"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY8269864.1"
                     /translation="MILSKRAQNLKTSPTLFLVAKAKELQAQGHPVISLTVGEPDWPT
                     FPAAARAGQEAIEKGITKYTAASGTVELRQAIVEKIKSEIGLDYGVKNVTVASGAKFI
                     IFGALQMLCSPGDEIIINSPYWVSYPTMVELADGVPVVVECGESDHFKMTPEKLESAI
                     TPKTRAFLFCSPSNPTGLVYTREELRALADVLVRHPQVMVISDDMYNRLMFDGTEIAP
                     HILQVAPELKERVVLVNGGSKAYSMTGWRIGWATGPERLITAMGDYQSQSTGSPSSIS
                     QYAALAALKHSEPDIAEVVKVLVQRKTTAMAEFKKLPQLRVIEPQGAFYLWVDMKSLY
                     GKTHKNKKIQGSKDFADILLNEYYVATVPGSECGCDGYLRLSFACSEGQMKEAIHRMA
                     SCLANLS"
     gene            complement(2586..3086)
                     /gene="coaD"
                     /locus_tag="VIG33_03190"
     CDS             complement(2586..3086)
                     /gene="coaD"
                     /locus_tag="VIG33_03190"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY8269865.1"
                     /translation="MSKLAVYPGSFDPITLGHVDIIQRISHLYEKTVVLISKSPDKAS
                     LFTAEERKELIEKSLSHLKNVSVDIHDGLTIDYMKEKKSDVIVRGLRAFSDFEHELTM
                     ANMNKMLAPKIETLLIFSDPKYYFISSRGVKEVAQHGGSLQGLVTDVVLEALVNKRKK
                     AMEKIK"
     gene            complement(3079..3648)
                     /gene="rsmD"
                     /locus_tag="VIG33_03195"
     CDS             complement(3079..3648)
                     /gene="rsmD"
                     /locus_tag="VIG33_03195"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEY8269866.1"
                     /translation="MRIISGKYKGHPLVEFKADHIRPTTDRVKETLFNKLMFDIEGSR
                     VIDLFSGTGNLGIEALSRGAQEVIFVEKHPKSLDILKKNLAKLKVPSSDYRIINMEVL
                     TYLKSYQGEAFDIVFADPPFTEKMAHAVMEQINQSQAFGPQTLMVIESQRKERIEDSY
                     GDIERYDHREFGDKILSFFAKKKDKEIHE"
     gene            complement(3645..3998)
                     /locus_tag="VIG33_03200"
     CDS             complement(3645..3998)
                     /locus_tag="VIG33_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8269867.1"
                     /translation="MKILIIDDEPLVRRSLGRALKSHGHDIFEAEDGLQGLNLWREQK
                     PELIFLDVLMPKMTGPQVLIEIGSERTGKVILISAFSGEHNMETATQMGADLFVPKPF
                     EDIFAIINLAEGLVK"
     gene            complement(3976..6027)
                     /locus_tag="VIG33_03205"
     CDS             complement(3976..6027)
                     /locus_tag="VIG33_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8269868.1"
                     /translation="MNQNDRHSADPILWVSTEPLPLEYRSLVLVAKVDWAEAQSTLQA
                     ERLLQSGRFSVVLISTDLDENIALDLIFAAKLHQAFSSRILRSKELTKRIIREGINRG
                     QVLKIIPQDLDARTFQGILNGALLKHLQRSQQSQLLKELSQRNRELEGLTNSLEQIVE
                     ERTLHIEISHTEETEKLSRERLLIRFIKELASQGSFEDILQVLRKELRKFHKLGDPIL
                     AYRVAGAKTELMSFHGGQFTQSESTIDMEFPEKYHSHHPELVRLFANHYGRPFIKTLI
                     FPLELKLIRQLAEGKAEAILCFESSLADKELNFFLDYVNDRLGPLSMALDRVLLENQL
                     SSFSYRWEKTFDSMKDPIAIVDVDYEVVRANKKFAEHNLGKKCHETFAGNKEICPACP
                     VSQALKNKASETGQIKVGTKTYQVYAYPVVSESGGRSTNVVNQYVDITESRQLYLKII
                     QSEKMGAIGKLAGHIAHELNNPLTGLRSLAQVLLQEAPKGDSLYSDLVEIEKAALRSQ
                     KIIKNLLEFSQNESQPQALISVDEIVEKTLPLLKTALRSHRFQLYLKSRHALVEVEPH
                     LLQQVVFNLVNNACQAMKTPGTLTVTAAISPSNPKNVELSVADTGPGIPKDIQNKIFE
                     AFFTTKKEGLGTGLGLSMSKSIVERFGGSIRFESEIGKGAVFCITLPIKEMTHENINH
                     R"
     gene            complement(6014..7462)
                     /locus_tag="VIG33_03210"
     CDS             complement(6014..7462)
                     /locus_tag="VIG33_03210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY8269869.1"
                     /translation="MKILVLQLARLGDIYMTWPVLRAIKRIHPTCELHLLTRSKFEAA
                     TEGLKSVDLHIQLPTASILEPLIQPTPDVEASLERLSPFIDKLKGEEYDWVINFTFSP
                     VSSYLTHAISAANTRVTGYTRHSDGFLSIPDEVSAYFYGQVGVDSFNRVHLTDIFISM
                     LGYEYVDADWDPPQVDGRDFSLPEKFLLVHIGASDIKKSMPPFKWARVFKNFFDHNLE
                     TPIVMIGSTAERALGEAIEAGVSRGRVINLIGQTHLREVFHLLMSTQLLVGCDSAPMH
                     MAGLTNTPCLNISFDTVNFWETGPKATNSFIYQASFPEDVVSEELGSYIHQVLQGEIP
                     TQLIQRSGGLSSYKLPKEDPQELFAWNLVQALYLNGNFPVNDNMQFYQAVEKINDINN
                     LARENIHMIPDKGLEVMGPLIDRADEVLAAVARVIPTVLPIIKWYQTEKVRIPPGSME
                     SVIQRSLEIHERLQRILSVYLLEDSIKAHESK"
     gene            complement(7558..8808)
                     /locus_tag="VIG33_03215"
     CDS             complement(7558..8808)
                     /locus_tag="VIG33_03215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY8269870.1"
                     /translation="MIILGISLLRLGDLVLQRPLIEGLRKKHPQCEIHMLINKQFTHV
                     EFLFEGLVDHFIYFDRESLQKSCGEREYNILWGYHQLKVLVEELNHIPYEAVYNFTHT
                     RLTAHLMGLIKARHRIGIYSYEGRFYGLNNPWIQFFNNYFGRPEAAGFHYTELLARAL
                     AIPLQQKFLKGNRTVAQQTVLIQGLTSDSKKNWELSKFQSLVRSLEKETLFEVKVLGA
                     PFERELLNKVFADKNLLICDFKEAAIALQNCALLITGDTSIKHLGALYDVPILELALG
                     SSQPLQVGAYSNNSIILQSRVTCGPCGHSQKCSQPIHRCGETLSVAAVYEAARIQLNL
                     KRMDWENFSNEHSELNTLRTQIHGVMGWSVQCLSLTEKYKFDEVIQKKTMVVEELNRQ
                     ATISKGESYERRIGKLSVNGAEAP"
     gene            complement(8805..9428)
                     /locus_tag="VIG33_03220"
     CDS             complement(8805..9428)
                     /locus_tag="VIG33_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269871.1"
                     /translation="MSIISFSENAEGYLIPFEDKKALCDFSNPQEEAERWVASQNMTI
                     SNEIVLLGLGAGFHLRALKEARSYLKIHVIECRDQLLPIYKKQIQSQVENYSCFHSLE
                     ALLDSEVFETTLIKSLPVYFLRSSWGAQDDLFYSFWNNLTGRSVYAIAKHFASLGIEI
                     DEGTLVSLKQSERPLTIKDATEIIIKSESSAAKESRGYFETLRELLR"
     gene            complement(9425..10489)
                     /locus_tag="VIG33_03225"
     CDS             complement(9425..10489)
                     /locus_tag="VIG33_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8269872.1"
                     /translation="MPELLLTNKSRDFSTGLESHPVAEPVPPPVYFDREDALREFDHE
                     TELAQLQSAQERIPDEKGAVLLANARVLMKHQEFSLALNLLRTATNHYSKNTTVLNLL
                     AQCLESTHCFNEALKVRLASMRILCNFDQVQKVATLYYKLGKDEEALHQYYEALSLLT
                     EEDEAIFEIYKNMGNILVRQGDFESAEDYYNKAYTLNPSSDVLLVNFGTLEVQRQDFD
                     KSLYCFRQAVEINSKNDKAWVGLAMVHAQFGDHQLAWANLETAIDINPANRTAVLLLA
                     NWGLRDHREGQSIEVLQEYLGNVEFDEDLSLVLINLYCSTSQIVEAKLECERVLLWNP
                     DNQEVASLREKLKKFKKACI"
     gene            complement(10651..11061)
                     /locus_tag="VIG33_03230"
     CDS             complement(10651..11061)
                     /locus_tag="VIG33_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269873.1"
                     /translation="MLRIINLLQEKNHYLEKFYALNETEIVNFSLGNFENLENFYNTR
                     ERILEIIKYIDECLNQTDLSTREVEEIHQVKLALRTKDEYVSRIIEQDLEVLACIEAA
                     KSNIIRELQDVRRVRKAVGGYKMPSFINRLDEEA"
     gene            complement(11070..11465)
                     /gene="fliS"
                     /locus_tag="VIG33_03235"
     CDS             complement(11070..11465)
                     /gene="fliS"
                     /locus_tag="VIG33_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HEY8269874.1"
                     /translation="MKNAYQKYKTQSIQTASKEKLLLMLYEGAIRFIKQAIKATEEKN
                     IAERGYNIGKAFDIIMELNNTLDHKVGGDIAMNLEQLYMFVMEQFTKANITGTTEPLQ
                     ASLKIIENLYSGWVQAVEKIKKDETESKK"
     gene            complement(11486..>12314)
                     /gene="fliD"
                     /locus_tag="VIG33_03240"
     CDS             complement(11486..>12314)
                     /gene="fliD"
                     /locus_tag="VIG33_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEY8269875.1"
                     /translation="ATGNDKQIEFPKIYMLDGDQDMYFEDSKPAQNAKVKVDGFEIEL
                     PDNKVSDLFPGVNLDIRQAAPGRSIRINVKENLEVISGKIKSFVDAYNAALGFIQTQH
                     KLSTGNSKTPQMGPLGGDGLLRTVESSLRSVILNPQYGVQTPIQRLNELGIEFNRNGT
                     LDFKQDKFNKVLQGNPQGVADFFRGDGMRTGFVATVKTAINNLVNNQFGPIGNRKKGM
                     QDKIDQINKRIDTKERQLVQKEDSLRKKFSDLESKMSKLNQQMGSVQSMAAAGNNKG"
BASE COUNT         3351 a         2952 c         2480 g         3531 t
ORIGIN      
        1 caatttattt tctcttagtt atttttttaa tcggctgcgg aaatgtgcgt ttcaccgcca
       61 gcacttcccc ggagggctct gggagtccca caggtgttgc aaccacaggg aataatgatc
      121 ccaatacagg agatgatgga aacactcccg ggccaacccc cacccccccg ccgccaccaa
      181 acggacctcg agatgttcac tactcaaaag cggtgccagc ggctagcaat caagtcgact
      241 ttcttcttgt tattgataac tcggcatcta tgatcgccat tcaacaaaaa atggccgcct
      301 caatgactgc cttggcaaaa caactcaacg gcttggccat tgactggcag atgtgcttaa
      361 ccttgacgac tgatatgaat aaaggtggtg ttgccaccaa ttcaggaact catacttgtg
      421 gatccgatgg atactgtaca ggcagttaca ctggtggttc ctggagttgg ggtgtttctc
      481 gcccatggtc tagtgactat acccccactg gcggagcttc acagtatatt ttaaagaaaa
      541 caacgaatga agccttatta aataaaatct tcaccaatac cattcctaag ctgggttcgg
      601 gggatacagg aactggtgat gaacgtggca ttaaggccgc ctacaatcac tttgcaaatt
      661 ccgcaaataa tggcgctttt aataccgctg gttgctatcg ccccggttct tctgttgcgg
      721 tgattgttct gtccgatgaa gatgagcgca gtgttgctgg agattgttcg cgagtaaaca
      781 ccgctcttgg ggatcacgaa attcccgcat gtcgatatta tgaagctccc cataacatca
      841 gaactttaga attcgctgat cagcctgcga atttactgtc gcaagccaaa aatatatttg
      901 ggtcctcctc gcggtttact tttaactcaa tcatcgccga cacagatgcc tgtcaaaatc
      961 agctcaatga caatatcagc ttccaagtga acggagtcag ctattactcc cctcactata
     1021 ctggcacagt ctacaaagcg gcctcgcagt taaccaatgg aggcgtggca agcctttgtt
     1081 caagcgattt acaattgaat ttatttggtg atgttgtagt gaacaccctc gcaaaagtaa
     1141 ctttggagtg tgctcccgcg aatctatcaa ttcaagttgg agccaacgag gccaatgttt
     1201 cctctctcga cccaagcaaa tattcggtga gtggcgccgt cgtcactttc aaccctgact
     1261 taacgcaaaa ccagtggttg aagatggatt accaatgtac acagtaaggc caattaaaag
     1321 tcaactttct gactagctta agttagccaa acaagaagcc atcctgtgga tggcttcttt
     1381 catttgcccc tcggaacatg cgaagctgag acgtaaatat ccatcacatc cacactccga
     1441 tcctggaacc gtcgccacat aatactcatt caaaagaatg tccgcgaaat ctttggatcc
     1501 ttgaattttt ttattcttgt gagtttttcc gtaaagggat ttcatgtcca cccaaaggta
     1561 gaatgctccc tgaggttcaa tcactcgcaa ttgcggaagc tttttaaact ccgccatagc
     1621 ggtggttttt cgttgaacca aaaccttcac cacttcggca atatcgggtt cagaatgttt
     1681 aagagccgca agcgccgcat attgagagat gcttgagggt gatcccgtgg actgactctg
     1741 ataatcgccc atcgctgtga taagcctctc ggggcccgtc gcccaaccga tgcgccatcc
     1801 tgtcatcgag taagctttag agcctccatt gaccagcacc acgcgctctt ttaattctgg
     1861 agctacctgc aaaatatgag gcgctatttc cgtgccatca aacatgagac gattgtacat
     1921 atcatcagag atcaccatca cctgaggatg gcggaccaaa acatccgcta aggccctgag
     1981 ttcctcgcga gtgtaaacca aacccgttgg attgctaggg gaacaaaaca agaaagcccg
     2041 agtctttggt gtaatcgcac tttccaattt ctccggagtc attttaaagt gatcagattc
     2101 accacactcc accactacgg gaacgccatc agcgagttcc accattgtag gatagctgac
     2161 ccagtaagga gagttgatga ttatttcgtc tccggggctg caaagcattt gtaaagcacc
     2221 gaaaataata aacttcgcac ccgaagccac cgtgacattt ttaactccgt aatccaggcc
     2281 tatttcactt ttaatttttt caacgatcgc ttgacgaagt tccactgtgc cactagccgc
     2341 agtgtatttg gtaattcctt tttcaatggc ttcctgacct gcgcgtgccg ctgcagggaa
     2401 ggtgggccaa tcaggctctc cgacagtcag agaaatcact ggatgtccct gggcttgaag
     2461 ttctttagcc ttcgccacca agaaaagtgt tggagaggtt tttaaattct gcgctctttt
     2521 ggataaaatc attggatcct cttcttcgtt ccttgaattt tctttattta atttctttat
     2581 ctaatttact taatcttttc cattgccttt tttctcttat ttacaagagc ctccagaaca
     2641 acgtcggtca ctaacccctg caaagatccg ccatgctgag ctacttcttt cactcctcga
     2701 gaggaaataa aatagtattt aggatcagaa aaaataagta aagtttcaat tttaggggcg
     2761 agcattttat tcatattcgc catggtcagc tcatgttcaa aatctgaaaa agcgcgcagg
     2821 ccccgcacga tcacatcgga tttcttttcc ttcatataat caatggtaag cccatcatgg
     2881 atatcgacgc tcacattttt tagatgagaa agacttttct cgatgagttc cttccgctct
     2941 tcagccgtaa agagagaagc cttatccggt gatttggaaa tcaacaccac ggttttttca
     3001 tagagatggg aaattctttg gataatatca acatgtccga gagtgatggg atcaaaactt
     3061 cccggataaa ctgcgagctt actcatgaat ctctttatcc ttttttttcg cgaagaaaga
     3121 taggattttg tcaccaaact ccctatgatc ataacgctct atatcgccat aagaatcttc
     3181 aattctttcc ttccgttgag attcaataac catcaaagtc tgcgggccaa aagcctggct
     3241 ttgatttatt tgttccatga ctgcatgagc cattttctca gtgaatggag gatccgcaaa
     3301 gacgatatca aaggcttctc cttgataact cttaagatag gtcagaacct ccatatttat
     3361 tatgcgatag tcactgctcg gaactttaag ctttgccaaa tttttcttca aaatatcaag
     3421 ggatttagga tgtttttcaa caaagatcac ttcctgagcc ccgcgcgaca acgcctcaat
     3481 ccctaaatta cccgtgcctg aaaatagatc aataactctg gaaccctcaa tatcaaacat
     3541 cagtttatta aataaggtct ctttcactcg atccgtggtg ggacggatat gatctgcttt
     3601 aaactctacg agaggatgcc ccttatattt tccagagata attctcattt cactaaacct
     3661 tcagctaaat taatgatcgc aaaaatatcc tcaaaaggct tgggaacaaa aaggtccgct
     3721 cccatttgcg tggcagtctc catattatgc tctcctgaaa aggccgaaat caaaatgacc
     3781 ttgcccgttc tttcgctgcc gatctcgatt aaaacttggg gccccgtcat ctttggcatt
     3841 aaaacatcca aaaaaatcaa ttcaggtttt tgttctcgcc agaggttcag tccttgcaat
     3901 ccatcttcgg cttcgaaaat atcatggcca tgacttttta aagcgcgtcc caaagatctg
     3961 cgaactaaag gttcgtcatc tatgattaat attttcatgt gtcatctctt taatgggaag
     4021 agttatacaa aataccgcgc ctttgccaat ttcagattca aaacggatgc taccgccaaa
     4081 tctttccaca atcgacttcg acatacttaa accaagacct gttccaagtc cttctttttt
     4141 tgtagtaaaa aaagcttcga aaatcttatt ttgaatatcc ttgggaattc caggtccggt
     4201 atcagcgacg ctgagttcta cattttttgg attgctgggt gaaattgcgg cagtgacggt
     4261 caaggttcca ggggtcttca tcgcttggca ggcattattg acgagattaa acaccacttg
     4321 ttgcagcaaa tgaggctcga cctccaccaa ggcatgacgc gatttcaaat aaagctgaaa
     4381 acggtgtgaa cgcaatgccg tttttaacaa ggggagagtt ttctccacaa tttcgtcgac
     4441 ggaaatcaat gcctggggct gactctcgtt ttgtgaaaac tctaaaaggt tcttaatgat
     4501 tttctgcgaa cgcagcgctg ctttttcaat ttccactaaa tcggaataca aagagtcccc
     4561 tttaggagcc tcttgcaaca aaacctgggc aagggagcga agacccgtca ggggattatt
     4621 caattcatgg gcgatgtggc ctgcgagttt cccgatggca cccatttttt cactctgtat
     4681 aattttaaga taaagctggc gagactcagt aatatccaca tactggttca ccacattggt
     4741 ggaacgccct ccgctttcgg acactaccgg gtaagcgtaa acttggtagg tcttagtacc
     4801 tactttaatt tgccctgttt ccgaggcctt gtttttcaaa gcctgactca caggacaggc
     4861 tggacaaatt tctttattgc ctgcaaaggt ctcatggcac tttttgccaa gattgtgctc
     4921 agcaaatttc ttattggcac gaaccacttc ataatcgacg tcgacaatcg cgatagggtc
     4981 cttcatgctg tcgaaggttt tttcccaacg ataagaaaaa cttgaaagct gattttctaa
     5041 aagcacccga tccagtgcca tactcagagg ccccaatcgg tcattcacgt agtccagaaa
     5101 aaagttaagc tctttatcgg caagcgaact ttcaaaacaa agaatagcct cagctttgcc
     5161 ttcagcgagc tgacgaatca gtttcaattc caatggaaaa ataagagttt taataaatgg
     5221 tcgtccgtaa tgattggcaa agagtctcac cagctcggga tgatggctat gatatttttc
     5281 aggaaactcc atatctatcg tactttccga ctgagtaaac tgtcccccat ggaagctcat
     5341 gagttctgtc ttcgcccccg ccactcgata ggcaagaatg ggatcaccca gtttatgaaa
     5401 cttacgcaat tctttgcgca aaacctgtaa aatatcttca aaagaacctt gcgaagccag
     5461 ctctttaata aaacgaatga gtaaacgttc gcggctgagc ttttccgtct cttccgtgtg
     5521 cgaaatctca atatgtaaag tgcgttcttc cacaatttgt tcgaggctat tggtcagccc
     5581 ttcgagctcc cgattgcgct gggataattc cttcagcaac tgggactgct gggaacgttg
     5641 caaatgcttt aagagggctc cgttcaagat tccttgaaaa gtgcgtgcat ctaaatcctg
     5701 gggaatgatt tttaaaacct ggccgcgatt aattccttca cggataattc tctttgtaag
     5761 ttctttggag cggagaatac gacttgaaaa ggcttgatga agtttcgccg cgaagatcag
     5821 atccaaggcg atattctcat ccaaatccgt agaaattaat acgactgaaa aacgcccact
     5881 ttgcagaaga cgttccgctt gaagggtgga ctgagcttcc gcccaatcca ccttcgccac
     5941 caagaccaag cttcgatact ctaaaggtaa gggctccgtc gaaacccata aaatagggtc
     6001 agcagagtgg cggtcatttt gattcatgag ctttaatgct atcttcaagc agataaacgc
     6061 tcaagattct ttgtaatctt tcgtgaattt ccaaactccg ctgaatgaca ctttccatgg
     6121 agccgggagg aatgcgcacc ttctccgtct gataccattt aatgatcggc aataccgtag
     6181 ggatgacccg cgcgacagcg gccaagacct cgtccgctcg gtctatcagt ggccccatca
     6241 cctccagacc tttgtctgga atcatatgga tattctcacg agccagatta tttatgtcat
     6301 tgattttttc aacagcctga taaaactgca tgttatcgtt caccggaaaa ttcccgttca
     6361 gatagagagc ttgaactaga ttccaagcaa aaagctcttg aggatcttct ttgggaagct
     6421 tgtaactcga caaccctcca cttcgttgaa tcaattgcgt tgggatttcc ccttgaagaa
     6481 cttggtgaat ataagaccct aactcttcag acaccacatc ctcaggaaag cttgcttgat
     6541 aaataaaact gttcgtggct tttggtccag tttcccaaaa attgaccgta tcaaagctga
     6601 tattgagaca aggagtattc gtcaagcctg ccatatgcat tggcgcacta tcacatccca
     6661 cgagaagctg cgtgctcatg agcagatgga agacctcgcg caaatgggtt tgaccgatca
     6721 ggttaataac ccgtccacga ctgactcccg cttcaatggc ttcgcctaag gctctctcgg
     6781 cagtggagcc gatcataaca atcggtgttt ctaaattgtg atcaaaaaag ttcttgaaca
     6841 ctcgagccca cttaaacggt ggcattgact ttttgatatc tgaagctccg atgtgcacga
     6901 ggagaaattt ttccggcaag ctgaaatccc ggccatccac ctgtggcgga tcccagtccg
     6961 cgtcgacata ttcatagcca agcatagaaa taaaaatatc cgttaaatgc actcgattaa
     7021 aagagtccac accgacttgg ccataaaagt aggcactgac ttcatcagga atacttaaaa
     7081 agccatcact atgacgggtg tagcctgtca cgcgggtatt cgctgccgag atcgcgtgag
     7141 tcaaatagct ggagaccgga gagaaagtga aattaataac ccagtcgtac tcttctcctt
     7201 tgagtttatc gatgaaaggg ctcaaccgtt ctagagaggc ctccacatcc ggagtcggct
     7261 ggatcaaagg ctccaaaatt gaggccgtcg gcaattgaat atggagatcg acggatttaa
     7321 gcccttcggt cgctgcttca aacttggaac gcgtcaacaa atgaagctcg caggttgggt
     7381 gaattctttt tatagctcgg agcacgggcc aggtcatata aatatcgcca agtctagcga
     7441 gttgcagaac tagaattttc atgatgaccc tgttctttga cgagttcagt gagaattttc
     7501 aacttttccg attgttcatt atagccctga gtttttaata cctctttata agctttctca
     7561 aggcgcttca gcgccgttga cagataattt tccaattcgt cgctcataag actctccctt
     7621 gcttattgtt gcttgccgat tcagctcttc aacaaccatc gttttttttt ggataacttc
     7681 atcaaattta tatttttcag tgagcgataa acattgcact gaccaaccca taaccccgtg
     7741 gatttgagtt cgcaaagtat tcagctctga atgttcatta gaaaaatttt cccaatccat
     7801 tcgttttaaa ttcaactgga tcctggccgc ctcatagacc gctgccactg agagagtctc
     7861 tccacagcga tgaataggtt gagaacattt ttgtgaatga ccgcaaggcc cgcaggtcac
     7921 ccgactttgc agaataatgg aattattgga ataagcccct acttgcaaag gttggctgct
     7981 gccaagcgcc agctcaagaa tcggcacgtc gtaaagagcc ccaagatgct ttatactggt
     8041 atctccagtg ataagaagtg cgcagttctg gagcgctatc gccgcctctt taaagtcaca
     8101 gatcaataaa tttttatcgg caaacacttt attcagaagt tcccgttcaa acggagcccc
     8161 cagcactttg acttcaaaca aggtctcttt ttcgagagat cgcaccaaag attgaaactt
     8221 agaaagttcc caatttttct tgctatcact tgtcaatccc tgaataagca ctgtctgttg
     8281 ggcaacggtg cggttccctt tgagaaattt ttgctgcaga ggtatcgcca gggcgcgggc
     8341 cagcaattcc gtatagtgaa atcccgccgc ttcgggacgc ccaaaatagt tattgaaaaa
     8401 ttgaatccaa ggattattta aaccgtaaaa ccgcccttca taagaatata tacctatacg
     8461 atggcgtgcc ttgatcaatc ccattaagtg agcggtcaag cgagtatgag tgaagttata
     8521 gacggcttca tacggaatgt gattcagttc ctccaccaga actttcagct gatggtaacc
     8581 ccatagaata ttgtattccc gctcaccgca acttttttgc agcgactcac gatcgaaata
     8641 aataaagtga tcgaccaatc cctcgaaaag aaattcaacg tgcgtgaact gtttattgat
     8701 caacatatga atttcacatt gaggatgctt ttttcgcagc ccctcaatca gaggtctttg
     8761 taagaccaaa tcccctaatc ttaaaagcga aattcctaat ataatcatct caacaattcc
     8821 cggagagttt caaaatagcc acggctttct ttcgcggccg agctttcaga tttaattata
     8881 atttccgtgg catcttttat agtcagaggt ctctcacttt gtttcaaaga caccaaagta
     8941 ccctcatcga tttcaattcc caagcttgca aagtgttttg caatggcata aacagaacgt
     9001 cctgtcagat tattccagaa ggaatagaac aaatcgtcct gagcgcccca agaactcctt
     9061 aaaaaataaa cgggaagaga ctttatcaga gtggtttcaa aaacttctga atccaaaagc
     9121 gcctccagac tatgaaaaca agaatagttt tcaacttggg actgaatttg cttcttgtag
     9181 ataggcaaaa gttgatcacg gcactcgatg acatgaattt tgagatagga gcgtgcttcc
     9241 tttaaagccc gcagatgaaa gccggcaccg agtcccaaca gaacgatctc attagaaata
     9301 gtcatattct gggaggccac ccatctttca gcttcttcct gaggattgct aaaatcacaa
     9361 agcgcttttt tgtcttcaaa ggggattaaa tacccctctg cattttctga gaaagaaatg
     9421 atgctcatat acacgccttt ttaaattttt ttagtttttc acgtaaagag gccacttctt
     9481 gattgtcggg gttccaaaga agaacgcgct cacactccag ctttgcctca acaatctggc
     9541 tagtactgca ataaaggttg atcagcacta aagagaggtc ctcatcaaac tccacattgc
     9601 caaggtattc ctgaagcact tcaatgctct gaccttcgcg atgatcgcga agtccccaat
     9661 tcgctagaag caagaccgct gttctattag caggattgat gtcgatcgcc gtttccagat
     9721 tggcccaagc aagctggtga tcaccgaatt gcgcatgaac catcgccagc cccacccatg
     9781 ccttgtcgtt cttagagttg atttccaccg cttgacggaa acaataaaga gatttatcaa
     9841 aatcttgacg ctgcacttcg agagtcccga agtttactaa aagcacatcc gaacttgggt
     9901 ttaaagtata agccttgtta tagtaatctt cagcactttc aaaatcacct tggcgaacta
     9961 aaatattccc catattttta tagatttcaa aaatggcttc gtcttcctct gttaagagcg
    10021 aaagtgcttc gtagtattgg tgtaaagctt cttcatcttt tccgagtttg tagtaaagcg
    10081 tcgccacctt ttgcacttga tcaaaattac agaggattcg catactggct agacgcactt
    10141 tgagcgcttc gttaaaacaa tgggtgcttt ccagacactg cgccaacaaa tttaataccg
    10201 tggtgttttt agaatagtga ttcgtcgccg tccgaagcag attgagcgcc aaggagaact
    10261 cttgatgctt catgagcact cgcgcattgg caagaagaac cgcccccttc tcatcgggga
    10321 ttcgctcttg cgcactctgt agttgcgcca gctctgtttc atgatcaaat tcacgaagcg
    10381 cgtcttcacg atcaaagtaa accggaggtg gaaccggctc cgcaacaggg tgactttcaa
    10441 gacctgtaga aaagtctctg gacttattgg tgagcagtaa ttcaggcatc gggacccttc
    10501 tcctttaatg gacgaaacca gcgtccacaa attggaaaag caaccctaat gccaagccta
    10561 ttttatattt cagcctgatt taagatcgat ctggcatccc gatgagccat gagtttgact
    10621 ctaaaggggg gccagatgtc atgaaattga ctaagcttcc tcatccaaac gattgatgaa
    10681 gctcggcatt ttatagcccc cgaccgcttt acgaactcga cgcacatcct gcagttcgcg
    10741 aataatattc gacttcgcgg cttcaataca tgccagcact tcaaggtctt gctcgatgat
    10801 acgactgaca tattcatctt tggtacgcaa agccagtttc acctggtgaa tctcttccac
    10861 ttctcttgta cttaagtcag tttgattcaa acactcatca atatatttga tgatttctag
    10921 aattctctcg cgagtattat aaaaattttc caaattctcg aaatttccta gagagaaatt
    10981 gacgatttcg gtctcattga gagcataaaa cttttccaag tagtgatttt tttcctgcaa
    11041 caggttgata attcgtaaca ttggctctcc tattttttag attcagtctc atcttttttg
    11101 attttttcca ctgcttgcac ccaaccggaa taaagattct ctatgatctt caaactggct
    11161 tgcaaaggct ctgtcgtgcc cgtgatattg gctttggtaa attgctccat gacgaacata
    11221 tagagttgtt cgaggttcat tgcgatatcg cctccaactt tgtggtccag cgtattgttc
    11281 agctccatta taatatcgaa agcctttcct atgttatagc cccgctcagc gatgtttttt
    11341 tcttcagtcg ctttgatcgc ttgcttgata aaacgaatgg ctccttcata gagcatcaaa
    11401 agcaattttt ccttactggc ggtctgaatt gattgcgttt tatacttttg ataggcgttc
    11461 ttcatagttc tccttttatt taaaactagc ctttattatt accggcggcg gccattgact
    11521 gtactgaacc catttgttgg ttcaatttcg acatctttga ctccaagtcg gaaaattttt
    11581 ttcttaagct atcttctttc tgcaccagtt ggcgttcttt ggtatcaatc cgcttattga
    11641 tctgatcaat cttatcctgc ataccttttt tacgattacc gatagggcca aactgattat
    11701 tcaccagatt attgatcgcg gtttttactg tggctacgaa gcccgtgcgc atgccgtccc
    11761 ctctaaaaaa atccgcgact ccctgcggat ttccctgcaa aaccttattg aatttgtcct
    11821 gcttaaagtc caaagttcca ttacgattga actctatgcc tagctcattc aatcgctgaa
    11881 tcggagtttg gactccgtac tgaggattta agatcacgct tcgaagagag ctttcaacgg
    11941 ttcttaaaag cccgtctcca cccaaaggtc ccatctgagg agttttactg ttaccagtac
    12001 ttaatttatg ctgggtctgt ataaatccca aagccgcatt ataggcatca acgaaacttt
    12061 ttattttccc gctgatgact tccaagtttt ctttgacatt aatccgaatg gagcgaccag
    12121 gagccgcttg cctgatatcc aagtttaccc ctggaaataa atctgagact ttattatcag
    12181 gcaactcaat ttcgaaacca tcaactttga ctttcgcatt ttgcgcgggc ttcgaatctt
    12241 caaaatacat atcctgatcg ccatccaaca tataaatttt tggaaactcg atctgtttgt
    12301 catttcccgt cgct
//
LOCUS       DATBTE010000084         7494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1033628, whole genome shotgun sequence.
ACCESSION   DATBTE010000084 DATBTE010000000
VERSION     DATBTE010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 7494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7494
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033628"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VIG33_03245"
     CDS             <1..853
                     /locus_tag="VIG33_03245"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HEY8269876.1"
                     /translation="PLLVTGSARIDTYKKVGDSMAGRYFQFRLFPLDIKELSQISRAD
                     PQKSLNTLLELSGFPEPYLSGDVREYKRWRNSHLDIILRQDMLETEAVKNIHSIELLV
                     ELMTERVGSLISYNSLREDLQTDDKTIKRWFEALENSYVFFKVPPYSKKINRAISKAP
                     KYYFFDIPRVSNDGARFENLVALSISKEIHYRKDVLGEDYSLHFLRNKNQNEVDFLIC
                     QEKRPLMMIEAKLSANNLENSFHIFEKGVGPIPKIIIVKNLDRSFTQKDGARVVKASE
                     WLEKMDF"
     gene            complement(1690..2670)
                     /locus_tag="VIG33_03250"
     CDS             complement(1690..2670)
                     /locus_tag="VIG33_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8269877.1"
                     /translation="MRNMKKVLVTGVAGFIANRVAESLLAEGVEVVGIDNLNDYYDVR
                     LKNYRLEQLQGKKGFQFLKMDIENREDLRDLFDKHQFDVVFNLAARAGVRYSMENPHV
                     YMTTNAMGSLNLLEEMKRKNIKKFILASTSSLYAGQKMPFSEDLAVNIPISPYAASKK
                     AAEVMAYSYHYLFGIDVSIVRYFTVFGPAGRPDMSIFRFIKWIDQGDRLEVFGDGAQS
                     RDFTFVEDIARGTILASKELGYEVINLGGGQNPISLNTVIAGIENRLGKKAAIDNRVF
                     HAADIKETWADISKAKRLLGWEPKISFEEGLEKTIQWYLQNKSWLKDIQL"
     gene            complement(2667..3926)
                     /locus_tag="VIG33_03255"
     CDS             complement(2667..3926)
                     /locus_tag="VIG33_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY8269878.1"
                     /translation="MRNVAVIGLGYVGLPLAVELSRKMPVVGFDISELRIKELSSGFD
                     RTREVETAQLKNSKLRYGSRLDDIAKSDFYIVTVPTPVHDSKQPDLTPLVKATESISK
                     VLKKGDIVVYESTVYPGCTEEICVPILEKNSGLKFNQDFFCGYSPERINPGDKERTIT
                     KIKKVVSASTPESLEVVDAVYASIIEAGTFRATSIKVAEAAKVIENSQRDINIAFVNE
                     LSLIFGRMGLDTREVLEAASTKWNFLKFYPGLVGGHCIGVDPYYLTFKAESLGYHPQV
                     ILAGRRINDNMGVHVAGQTVKLMIKKNLTVNNAQVLILGVTFKENCPDVRNSRVIDVI
                     NELKAFGCKVDVFDPHAIAEEVEHEYHIKPVSGDCLKNLSKYTAIVLAVGHNEFKEIH
                     WDQLKAEDHVVYDVKGLLPLEMVDGRL"
     gene            4200..4859
                     /locus_tag="VIG33_03260"
     CDS             4200..4859
                     /locus_tag="VIG33_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269879.1"
                     /translation="MNARAFFKKLGSENLSELERKTDLSRRALGSAKKTHNMKLENLE
                     VLADAMGFSVQFLPQESEDNVLLSLKRFGAPLAHESGGNLDLTEALVCGMNLARQDGL
                     YESIVPYVMAQNSQKLHPLDILSKVHLRGDHMKVVGYFAEMANHFKPHPHLAELMRLA
                     QVFNPDLKQKELLLKKEKVNFPELFEKNKTALRWGLLTRGSERDHLERWAKWQQSRKS
                     P"
     gene            4832..5392
                     /locus_tag="VIG33_03265"
     CDS             4832..5392
                     /locus_tag="VIG33_03265"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HEY8269880.1"
                     /translation="MATVTKESLETLFRNIDSKLEYTVDIYIIGGASAILGYNIVKQT
                     DDVDLDGTAEPHFQKLFNEASHETGTDLVLSSKGVFTPPDGYRERMHFKDFPRRKLRI
                     WFLDKDDFAISKISRGFGKDYEDISKVHEQAPYEYASLMNIFMTEFIGIATIGNEKET
                     KMNFLDLVEKLFGSERMIEAKRDIKF"
     gene            5500..6297
                     /gene="rfbD"
                     /locus_tag="VIG33_03270"
     CDS             5500..6297
                     /gene="rfbD"
                     /locus_tag="VIG33_03270"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEY8269881.1"
                     /translation="MILVFGKNGQVATALREILPQATFLGSDVANFLDLAAIKKSLDQ
                     HKPRVVINAAAYTQVDKAESEKEAALTINGTTPGVIAEWCKSHDALLVHYSTDYVFDG
                     SGEKPWLETDQPNPVNYYGFSKWQGEKAIQASGCKHYIFRVSWVYSPWGQNFKKTILR
                     LAQEREELNIVGDQWGAPTKAEDIARETKKVIEGYLNHKPLEFGLYHWRFNDYTTWHQ
                     FAVDIVEEAKKSGVVLKIKNLNSIPTEAYPTPAKRPKNSRLGTNRFS"
     gene            complement(6345..7229)
                     /locus_tag="VIG33_03275"
     CDS             complement(6345..7229)
                     /locus_tag="VIG33_03275"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEY8269882.1"
                     /translation="MNITKDIAIKRYKSESTGLSSGDQRNLIREILQETILCHMKEEN
                     LFNDFAFHGGTSLRILHRIDRFSEDLDLSLIEPNSFYAFPEKMSKLEKSLKKAGFTFE
                     FQNKCKPENPIKKYFINDTLFLNEFKDQIGNVIDGEKVKIKLELDVEPPEYQEFETFE
                     IKSKFAAKIYAHNLSTCMGQKIHAVLCRGQSYGLDIVKGRDLYDLDWYLKKGVKPNLK
                     NLSSCLNRLGPWKGKGLNVDAKWIVTSVNECLSTKDFDAILSDLKPLVSSRTFDPLKE
                     VWKREYFSKLLQGLLNSQ"
     gene            complement(7226..>7494)
                     /locus_tag="VIG33_03280"
     CDS             complement(7226..>7494)
                     /locus_tag="VIG33_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269883.1"
                     /translation="QLGFGIQIININGQQVRIADPVRAIMDTLIEQKLVPKNRNQICV
                     FLRIDEDESESIPWKNAEQYAYKYRNSKLAKKIAAAMTEPGLHV"
BASE COUNT         2218 a         1664 c         1490 g         2122 t
ORIGIN      
        1 ccctcttctc gtcacgggaa gtgcccgcat tgacacctat aaaaaagtag gggattccat
       61 ggcaggccgt tatttccaat ttcgactttt cccattggat atcaaggaac tctcacagat
      121 ctccagagca gacccccaga agtctctgaa tacacttttg gaactcagtg gttttccgga
      181 accttattta tccggcgacg tccgcgaata caaaaggtgg cgaaactcgc atttagatat
      241 tattttacgt caagatatgc ttgaaaccga agctgttaag aatattcatt ctattgaact
      301 cttggtggaa ttaatgaccg aacgagtggg cagcctcatt agttacaact cgcttcgcga
      361 agatttacag acagacgata agactatcaa aagatggttc gaagcccttg aaaactctta
      421 tgtttttttc aaggtcccgc cctattctaa aaaaatcaat cgcgctatca gtaaagctcc
      481 caaatattat tttttcgata ttccacgagt gagcaatgat ggagcacgtt ttgagaatct
      541 agtcgccctt tctatttcta aagaaattca ctatcgcaaa gatgttctcg gtgaggacta
      601 ttctttgcat tttctcagaa ataaaaacca aaatgaagtg gattttttga tctgccagga
      661 gaaacggcct cttatgatga ttgaagcaaa actttctgcc aacaatctcg aaaactcttt
      721 tcacattttc gaaaagggag tcggcccgat acctaaaatt attattgtca aaaatttaga
      781 tcgtagtttc actcaaaaag atggagcccg ggtcgtgaaa gcaagcgagt ggctcgagaa
      841 aatggatttt tagaatgggg tgcaactcct tcatcctagc cggagcatga tttatggctt
      901 ttaccaaaaa acacataacg cctggcaact ttaatgactt tagtcagaca ttatccagaa
      961 ttatccaggt aaaatatgaa gatgaatgag ctcctgcttt tttgagaaga gacttagcaa
     1021 gtgttgtttt gccaacctga cgagggccgg aaataaaggc aatctttttt gaagccaaag
     1081 catcatctat gataagatct tctaaatacc gctttagcat attattacta taatacaccg
     1141 ctatcgtaat ttggtaatac tattttacga tctatcgtaa tttggtaaat ataaagccaa
     1201 tctatcggtt ttaataagat ttcctagcaa cccaaaccaa attgctcaca tgcgtcaagt
     1261 cgatacgttt gatcaaattc ttgactccct tcccttaaca aacaaaacca ttaaattata
     1321 aagttaagta ttcgtcctag cggggccttc caaacctttg aagtcgatcg cgcaatatgc
     1381 tacttgcgag cgtatggcgc gatcgggttt taagtgtcct cgcggaacgc cagtctctgc
     1441 aactgtgcaa tgaaaaaaaa ctcacaataa aagtaaaaga tccatcggct ctctgttatg
     1501 ccaacttgac aaagatttgg ctaacataag tcgcgaacct atccatcctc ctcatgaaaa
     1561 tgaggagcgc gactttaact ttggttcgag tacgaaccaa agttccaata aataattcaa
     1621 atgcaaaggt tagacttact ggaaactcga gcataaaaac ctgagcaggt ttccttttgc
     1681 tggaactgac taaagctgga tatctttgag ccaactttta ttttgcaaat accactggat
     1741 ggttttctcg agcccttctt cgaagctgat ttttggttcc cagccgagaa gtcttttggc
     1801 ttttgaaatg tccgcccagg tttcttttat atcagccgcg tggaaaacgc ggttgtcgat
     1861 ggcggctttc ttgcccaagc ggttctcgat ccctgcgatc accgtgttca gactgatagg
     1921 attttgtcca ccacccagat tgatcacttc atagccgagt tctttgctcg caagaatggt
     1981 gccgcgggcg atgtcttcga cgaaagtaaa atcccgggac tgggctccgt caccgaaaac
     2041 ctccaggcgg tccccttggt ctatccactt gataaatcta aaaatcgaca tatcaggacg
     2101 tcccgcagga ccaaacacgg taaaataacg aacgatggaa acatcgatgc caaaaagata
     2161 gtgataagaa taagccatga cttcagcggc ctttttagaa gcggcgtaag gtgagatcgg
     2221 aatattcacc gcgagatctt ctgagaaggg cattttctgc ccggcatata aagaggaagt
     2281 cgatgctaaa ataaacttct tgatgttctt tcttttcatt tcctcgagaa gattcaaaga
     2341 gcccatggca ttggtggtca tatagacatg gggattttcc atgctgtagc gaacccccgc
     2401 ccgcgccgcc aaattaaaga ccacatcaaa ttgatgttta tcaaagaggt ctcttaagtc
     2461 ctcgcggttt tcgatatcca tttttagaaa ttgaaagcct ttctttccct gaagctgttc
     2521 caggcgataa ttcttcaggc gaacatcata ataatcattg agattatcaa tccccacaac
     2581 ttcgacaccc tctgccagga gggattcagc aactcgattg gcaataaatc ccgcaacccc
     2641 agtgactaaa acttttttca tatttctcat agacgaccat ccaccatttc caaaggcaat
     2701 aagcctttta catcatagac cacgtgatct tccgccttga gttggtccca atggatttct
     2761 ttaaattcat tgtgccccac tgctaaaacg atggcagtgt atttcgaaag atttttcaag
     2821 caatccccgg acacgggttt aatatgatat tcatgttcca cttcttcggc gatagcatgc
     2881 ggatcaaaaa catccacttt gcagccgaag gccttcaatt cattgatcac atcaatcacc
     2941 cggctatttc tgacatcggg gcaattttct ttaaacgtca cacccaagat taaaacctgg
     3001 gcattattca ccgtgagatt ctttttaatc atgagcttca ccgtctggcc ggccacatgc
     3061 acgcccatgt tgtcattgat acgacggccc gctaaaatca cttgggggtg atagcccaga
     3121 gactcggcct taaaggtcag atagtaaggg tccacaccga tgcaatgccc accgacgagt
     3181 cctggataaa actttaaaaa gttccacttt gtcgaggctg cctcaaggac ttcccgggta
     3241 tccaaaccca tgcgtccgaa aatcaaggag agttcattca cgaaagcgat attgatgtca
     3301 cgttgagagt tctcgatcac tttcgcagct tccgccactt taatcgaggt tgccctgaag
     3361 gtgcccgctt caatgataga ggcataaaca gcgtcgacca cttcgagaga ttccggggtc
     3421 gaggcactga cgactttttt aattttcgtg atggttctct ctttatctcc ggggttgatg
     3481 cgctcagggc tatagccgca gaaaaaatct tgattgaatt ttaagccgct gtttttttct
     3541 aaaataggaa cgcaaatttc ctcggtgcag cctgggtaaa ccgtggactc atagacaaca
     3601 atatcgcctt ttttcaaaac ttttgaaatg ctctcggtgg cttttaccaa gggggtcaaa
     3661 tccggctgct tactgtcgtg aaccggcgtc ggtacggtga cgatataaaa atctgatttt
     3721 gcaatatcgt ctagtcgtga accatagcgg agtttagaat ttttcagctg agccgtttcc
     3781 acttcccggg tgcgatcaaa accactggaa agttctttaa tcctgagctc actgatatca
     3841 aatcccacca cgggcatctt tcgcgacagt tccaccgcta agggaagtcc gacgtagcca
     3901 agtccgatga ctgcaacatt tcgcatacac acactcctaa gaaacgttcc acagcatacc
     3961 ttgaactgaa ctcgagggca aaaaccctga gaacgcgcat tgcattattc gaagcgatgc
     4021 gattcaggtt tgatgcgaaa atgtttctaa aaagtatagg gtaacattaa cagagtctaa
     4081 tgacgagaat tcgctcaata ttaagacatt ttaaccataa gtacctaata ttagatatat
     4141 ttttatctaa tattagatga gaagttgact tcccacatta tattagataa tctgccttta
     4201 tgaatgcacg agcatttttc aaaaaattag gcagtgagaa cctttctgag ttggaaagaa
     4261 aaaccgatct ttcgcggcga gcacttggca gtgcaaaaaa aactcataat atgaaacttg
     4321 agaacttaga agttcttgcc gatgccatgg gtttctctgt gcaatttctt ccgcaggaat
     4381 ctgaagataa tgtccttttg agtcttaagc gctttggcgc gcccttagct catgaatcag
     4441 gcggcaactt agatcttacc gaggccctcg tctgtggaat gaacctggcc cgtcaagatg
     4501 gtctgtatga atctatcgtt ccctatgtaa tggctcagaa ttctcagaag ctccatccct
     4561 tagatatact atctaaggtt cacttgcggg gggatcacat gaaagtcgtg ggctacttcg
     4621 ccgagatggc caatcacttc aagccacatc cccacttagc tgaactcatg cggcttgcgc
     4681 aagtttttaa tccagatctg aagcaaaaag aacttctctt gaaaaaagag aaggtgaact
     4741 ttcctgaact ttttgagaag aacaaaacgg cactaagatg gggacttctc actcgtggaa
     4801 gcgaaaggga tcatcttgag aggtgggcaa aatggcaaca gtcacgaaag agtccttaga
     4861 aaccttattc cgaaatattg attcaaaatt agagtacaca gttgatatct atatcatcgg
     4921 tggagcttca gctattcttg gctataatat cgtcaagcag acggacgacg ttgacctcga
     4981 tggcaccgcc gaacctcatt tccagaaatt gtttaatgaa gcctctcatg aaacaggcac
     5041 tgacctcgtc ttgtcctcta aaggagtttt cactccacca gatggttatc gggaaagaat
     5101 gcactttaag gatttccccc gtagaaaact tcgcatctgg tttctagata aggatgattt
     5161 tgcaatcagt aaaattagtc gcggctttgg aaaagattat gaggacattt caaaagttca
     5221 tgagcaagcg ccctatgaat acgcaagttt aatgaacatc tttatgactg agtttattgg
     5281 tattgcaacc atcggtaatg agaaggagac aaaaatgaac ttcttagatc tggtggaaaa
     5341 actctttggc agtgaacgta tgatagaggc aaaacgggat attaaattct aagaaaaaac
     5401 tactcaaaaa ccctgagaac gtgcattgca ttattctatt gtctaagtga aactaaatgc
     5461 ataaattatg aatccacatc actagctgaa ggatttttta tgattttggt cttcggaaaa
     5521 aatggtcagg tcgccacggc tctgcgcgag atattgcctc aggctacttt tttaggcagc
     5581 gatgtcgcca acttcctcga tctagccgcg attaaaaaat ccctcgatca gcacaaacca
     5641 agggtggtga tcaacgccgc tgcctacacc caagtggaca aagccgagtc agaaaaagaa
     5701 gctgctctga ccattaatgg gaccactccc ggagtgatcg cagagtggtg taaaagccat
     5761 gacgccctct tggtccacta ttcaaccgat tatgttttcg atggctccgg agaaaaaccg
     5821 tggctggaaa cagaccaacc caatcccgtt aattactatg gtttttcgaa atggcaaggg
     5881 gaaaaagcca ttcaagccag cggttgcaag cattatattt tccgcgtctc gtgggtctac
     5941 agcccctggg gacagaactt caaaaaaacc attctgcgct tagcccaaga gagggaagaa
     6001 ttaaacattg tcggcgatca atggggagcc cccacaaagg ccgaagacat cgcccgcgaa
     6061 accaagaaag tcattgaggg ttacctgaac cacaagcctt tggaattcgg actttatcac
     6121 tggagattta acgattacac cacttggcac caattcgccg tggacatcgt cgaagaggca
     6181 aaaaaatcgg gagtggtttt aaaaatcaag aacctcaact ccattccgac cgaagcttat
     6241 cccacaccgg caaaaagacc gaaaaactcg cgactgggga caaaccgctt cagctaaaat
     6301 ctctataggc gatttgcgat ccaagacttg gatagcttgg aatgttattg cgaatttaac
     6361 aggccctgaa ggagcttgct aaaatattcg cgcttccata cttcctttaa tggatcaaaa
     6421 gtccgactac ttactaatgg ttttaaatct gaaagaatcg cgtcaaaatc tttggtggaa
     6481 agacattcat taactgatgt tacaatccat ttagcatcaa cattaagtcc tttgcctttc
     6541 catggaccca agcgatttaa gcaagagctt aagtttttga gattaggctt aactcccttc
     6601 tttagatacc aatcgagatc atataaatct cttcctttaa caatatccaa accataggat
     6661 tgtccacgac aaagaaccgc atgtattttt tgccccatac aagtagacaa attatgtgca
     6721 tagatctttg ctgcaaactt acttttgatt tcaaaagttt caaattcttg gtattcgggt
     6781 ggttcaacat caagttctaa ttttattttg actttttctc catcaatgac atttccaatt
     6841 tgatctttaa attcattaag aaataaagtg tcattgataa aatatttttt gataggattt
     6901 tcaggtttgc atttattttg aaattcaaaa gtgaacccag ctttttttaa ggacttttcc
     6961 agtttcgaca ttttctcggg aaatgcataa aaactattag gttcaatcaa agacaaatca
     7021 agatcttcag aaaacctatc aatcctatgt agaattctta gcgaagttcc accatgaaat
     7081 gcaaaatcat taaaaaggtt ttcttccttc atgtgacaaa ggattgtctc ctgtaaaatt
     7141 tctctaatta aattgcgctg atcccctgaa cttaatccgg ttgattcact tttgtatctt
     7201 tttatagcaa tgtcttttgt gatattcata catgcaatcc aggctcggtc attgcagcag
     7261 caattttttt cgcgagtttt gagttcctat acttataagc gtattgctct gcatttttcc
     7321 atggaatgga ctctgactca tcttcatcaa ttctaagaaa aacacaaatt tgatttctat
     7381 tcttcggcac taatttttgt tcaatgagag tatccataat tgctctcacc ggatcagcaa
     7441 tacggacttg ctgaccgtta atgttaataa tttgaatacc aaatcccaat tgat
//
LOCUS       DATBTE010000085         4583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_965667, whole genome shotgun sequence.
ACCESSION   DATBTE010000085 DATBTE010000000
VERSION     DATBTE010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965667"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VIG33_03285"
     CDS             complement(<1..674)
                     /locus_tag="VIG33_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEY8269884.1"
                     /translation="MSEIFHRPKINLTRTLPVPNAYTLALDGEYSPVAFNEERAPLNK
                     GKWRSEVFKAEDAQPMDLEIGTGAGHHFAHYANGNPDRLVVGLELKYKPLIQTIRRAL
                     HGGSKNAAIARYHAFNIDELFVEGELNNVFIHFPDPWTSPKKPRNRVVNIRMLDLIYK
                     MQRPGSHIDFKTDSRVYFLWALEEIKQSKFKVVQQTLNLHQSEFKDQNFMTGFEKIFV
                     KQGIEI"
     gene            complement(677..1693)
                     /locus_tag="VIG33_03290"
     CDS             complement(677..1693)
                     /locus_tag="VIG33_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY8269885.1"
                     /translation="MKLTPVLITANRSRYKAAWISLLASTFVFAIKVAAYYVTHSTAV
                     LSDALESTVNVVAALVALIVVRIVSRPADEDHPYGHGKLEYFSAAFEGGLIFFAALMI
                     IRESLDALFSGHTSHQLESGLVIMIVAAVFNLVLGFYLKRIGEQEKSDALKASGAHVI
                     SDVWTSVGVVIGIVLVLVTGIQWIDPLVAIAVAMNLAYEGYKIVRKSAGSLIDEVDTE
                     VLNQLALSLEKNHRPGIIDVHKLKIIRSGRFHHVDAHLVVPEYWDISHAHNYCEDFEA
                     DVVADYPFDGEIAFHLDPCRRAYCERCKMTDCPIRVAVFEKRTPFTVQSLVGDPPIQE
                     KVEM"
     gene            complement(1735..2739)
                     /locus_tag="VIG33_03295"
     CDS             complement(1735..2739)
                     /locus_tag="VIG33_03295"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8269886.1"
                     /translation="MVRDNLVKSPKERKILVIDDDADSREIILEPLRWEHFDARGVSS
                     GIEALKLMETWVPNIIILDWMIPGISGLQILQTLRAHYPHVSCIFISGNSSTESIIEA
                     LDSGSDDYIIKPFVPLELLARIRTHFRIRDLHSQLLLANERLKELVDTDDLTGLFNMR
                     SFYQRLDFELERGKRFGRDVCVVMMDMDYFKTVNDGHDHLFGSYVLSEVGKVIRINTR
                     NIDIPARYGGDEFLVVLMEVDYRGAMHFCERLRNAIAERTFVSGNDSINLTLSLGFAI
                     TVQGEDISARELVRRADHALYEAKYAGRNQTRFYTAETDKVRELRPKPVPPTKKPAAG
                     "
     gene            2945..>4583
                     /gene="leuS"
                     /locus_tag="VIG33_03300"
     CDS             2945..>4583
                     /gene="leuS"
                     /locus_tag="VIG33_03300"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY8269887.1"
                     /translation="MALNHNEAERFKTFESKWRKRWEDQGAFRAENSSSKPKYYALSM
                     FPYPSGVGLHLGHIASYTPTDIMARYKRTVGFNVLNPMGYDAFGLPAEQYAIQTGIHP
                     AITTEKAIESFRKTLQSFGFSFDWSREISTCDAKYYKWTQFIFLKLMERGLAYQKEVP
                     VNWCPALKTVLANDEIIDGKSERGGHPVVRVPMKQWMLKITDYAERLLEGLDRVDWPE
                     RTKEGQRNWIGKSEGARVTFKIEGGAHSFEVFTTRPDTLFGVTFMVMAPENPLVQKIT
                     TSGQKSAVDNYVETSARKSEVDRKACTDKTGVFTGSFASHPLNGQKIPIWIADYVLMD
                     YGTGAIMAVPGHDVRDFEFAEKFKLPVIRVLEGGDSLPFEGEGTLVNSDFLNGLSKKM
                     AVQKMIEYLEAGKIGEKEIQYKLRDWLFSRQRYWGEPFPVVHFPKAGMKGVPVNELPV
                     LLPEVADYEPSDKGEAPLARNKEWVEYKGTAAIGAEGERGRRETDTMPGSAGSSWYFL
                     RYIDPHNDNAPFSAEAEKYWMPVDLYVGGPEHTVGHLL"
BASE COUNT         1311 a         1116 c         1004 g         1152 t
ORIGIN      
        1 ttaatttcaa tgccctgttt tacgaagatc ttttcaaaac cggtcatgaa attttggtct
       61 ttaaattcag actgatgcaa attcaaagtt tgttgaacaa ctttgaattt actttgtttg
      121 atttcttcca gggcccacaa aaagtaaacg cgagaatccg ttttaaaatc aatatgggat
      181 cccggccttt gcattttata aatcaaatcc aacatacgta tattcacgac acgatttctg
      241 ggcttcttgg ggctcgtcca agggtccgga aagtgaatga agacattatt gagttctcct
      301 tcaacaaaaa gctcgtcaat attgaaggca tgatatcgag caatcgccgc atttttactt
      361 cctccatgaa gagcccgacg aatcgtctga attaagggtt tgtacttaag ctccagcccc
      421 accactaagc gatctggatt tccattggca taatgagcaa aatgatgacc cgctcccgtc
      481 ccaatctcca aatccattgg ctgagcgtcc tctgctttaa aaacctcgga acgccatttc
      541 cctttgttca aaggagcgcg ctcttcattg aaggccacgg gagaatactc tccatcgaga
      601 gcaagagtat aagcgttagg aacaggtaaa gtgcgggtta aattaatttt tggacgatgg
      661 aatatttcag acatatctac atttcaactt tttcttgtat aggaggatca ccaactaaag
      721 actgcactgt aaagggagtt cgtttttcaa agacggcgac gcggatggga caatctgtca
      781 ttttgcacct ctcgcaataa gcacgcctgc agggatctaa atggaaagcg atctccccgt
      841 caaaaggata atcggcgacc acatctgcct cgaaatcttc acagtagtta tgagcgtgcg
      901 aaatatccca atactcagga accacgagat gagcatccac atgatgaaat cgccccgaac
      961 gaataatctt cagtttatgg acatcaatga ttcctggacg gtggtttttt tccagtgaaa
     1021 gagccaactg attcaagact tcagtatcca cctcatcaat gagacttccc gcggacttgc
     1081 gcacaatttt atatccttcg taggccagat tcatggcgac ggcgatggca acgagaggat
     1141 caatccattg gattccagtt accaggacca aaacgatccc aatgactacg cctacggaag
     1201 tccaaacatc agaaataaca tgggccccac tggctttcag agcgtccgat ttttcctgtt
     1261 caccaattct cttaaggtaa aatcccagaa cgagattaaa aaccgcggcc acaatcatga
     1321 tcactagacc cgactccaac tgatgcgaag tatgaccgct aaataaagca tcaagcgact
     1381 cgcgaataat catgagagct gcgaagaaaa tcagccctcc ttcaaaggcg gctgaaaaat
     1441 actctagttt tccatggcca tacggatgat cttcatcggc gggccgagat acgatacgaa
     1501 ccacgatcag agccaccaac gcagcaacaa catttacagt gctttccaag gcgtcagata
     1561 atacagctgt cgagtgggtc acataatatg ccgctacttt tatggcgaaa acaaaagtac
     1621 tcgccaaaag cgaaatccat gcagccttat aacgtgacct attggcggtg attaaaaccg
     1681 gggtgagctt catagaagct ttgtcttaaa ttcgcggctc tcagtcaatg agaatcaccc
     1741 agccgctggc tttttggtag gcggaaccgg cttcgggcga agctcacgga ctttatcggt
     1801 ctccgccgtg taaaaccgtg tttgattacg acccgcatac tttgcctcat agagagcatg
     1861 atcggctctc ctcactaact ctcgtgccga gatgtcttcc ccctgaactg tgatggcaaa
     1921 gcccaaggaa agggtcaaat taatcgaatc attaccactg acaaaagtac gctctgcaat
     1981 ggcattgcga aggcgttcac agaagtgcat ggcgcctcgg tagtccacct ccatcaaaac
     2041 aaccaaaaat tcgtcgcctc cataacgtgc cgggatatcg atatttcgtg tattaatcct
     2101 gatgacttta ccgacctccg acaaaacata actgccgaag agatgatcat ggccgtcatt
     2161 cacagtttta aagtaatcca tgtccatcat gacaacacaa acatcacgtc caaaacgttt
     2221 gccccgttcc aactcaaagt ccaaacgctg ataaaaagat cgcatgttga acaaacccgt
     2281 caaatcatcg gtatcgacca gttccttcag tctctcatta gccaaaagta attgggagtg
     2341 aagatcacga atccggaaat gagtgcgaat acgcgccaaa agctccaaag gaacaaaagg
     2401 tttgatgata tagtcatcag agcccgagtc caaggcttca atgatggatt ctgtgctgga
     2461 atttccggaa ataaaaatac aggaaacatg agggtagtgg gctcttaaag tttgtaaaat
     2521 ctggagaccg gaaatccctg gaatcatcca atccaaaatt atgatattcg gaacccaagt
     2581 ttccatgagc ttcaacgctt ctattcccga cgacacgcct cgagcatcga aatgttccca
     2641 acgcaacggc tcgagaatga tctctcggct atcggcatca tcatcaataa caagaatctt
     2701 ccgctctttt gggcttttaa ctaagttatc acgtaccata tggctctatt ttagcgcgct
     2761 ccctggaaaa acctgtacag aagcttaagt tcacggcgat ttctcatccg aagtccaggc
     2821 gaattcttag gcgaaaatcc aggaggacat ccgccggaga tttgcctaga attcttttcc
     2881 attttcattg aaagatccgc tattcttagc tattcatttt tagacatttt ttaacgagga
     2941 aacgatggca ttgaatcaca acgaagctga gagatttaaa acttttgaga gtaagtggcg
     3001 aaaaagatgg gaggatcaag gggcttttag agccgaaaat agctcttcta aaccaaaata
     3061 ctacgctctg agcatgttcc cttatccctc tggagtcggc cttcatctgg gccatatcgc
     3121 ctcttacact ccaacagata ttatggctcg ctataaacgc acggttggtt tcaatgtttt
     3181 aaaccccatg ggttacgatg cttttggtct ccccgcagaa cagtatgcca tccaaacggg
     3241 aattcacccc gccatcacca cggaaaaagc catcgagagt ttccgcaaaa cactgcagtc
     3301 ttttggtttt agctttgatt ggagccgcga gatctctacg tgtgatgcaa aatattataa
     3361 atggactcag tttattttct taaaactcat ggaacgaggc ctcgcctatc aaaaggaagt
     3421 tccagtcaac tggtgtccgg ctttgaaaac agtcttggct aacgatgaaa tcatcgatgg
     3481 caaatcggag cgcggtggtc atcctgtggt tcgggttcca atgaaacaat ggatgctaaa
     3541 aatcaccgac tatgcggagc gtttgcttga gggcctcgac cgcgtcgact ggccagagcg
     3601 cacaaaagaa ggtcagcgta attggatcgg caagagtgaa ggcgctcgcg tcacctttaa
     3661 aattgaaggt ggtgctcaca gttttgaagt cttcaccacc cgtccggaca ccttgtttgg
     3721 agtgactttc atggtgatgg ctcctgaaaa cccactcgta caaaaaatca caacctcagg
     3781 acagaaatcc gctgtggaca actacgtgga aacttccgca cgaaaatctg aagtcgaccg
     3841 taaagcctgt acggacaaaa ccggcgtctt cactggatct tttgcctctc atccgctgaa
     3901 tggtcaaaaa attcccatct ggattgccga ttatgttctg atggattatg gcaccggcgc
     3961 tatcatggca gtacctggcc atgacgtccg tgattttgaa ttcgctgaga agttcaagct
     4021 tccagtgatt cgtgttctcg aaggcggcga cagtttaccc ttcgaaggcg aaggcacgct
     4081 cgtgaactct gatttcctca atggcctttc taaaaaaatg gccgtgcaga aaatgattga
     4141 gtacttggaa gccggaaaaa ttggtgaaaa agaaattcaa tacaagctcc gcgattggtt
     4201 gtttagtcgc caacgctatt ggggagaacc ctttcccgtc gttcacttcc cgaaagccgg
     4261 catgaagggt gttcctgtta acgaacttcc agtcttattg ccagaggttg ctgactatga
     4321 accttctgat aaaggcgaag ctcctctagc tcgcaacaaa gaatgggttg aatacaaagg
     4381 cactgcggcc attggcgccg aaggtgagag gggccgacgt gaaaccgaca ccatgccggg
     4441 ctctgcagga tcttcgtggt atttcctccg ctacattgac ccccacaatg acaacgctcc
     4501 gtttagtgcc gaagctgaaa aatactggat gcccgtcgat ttatacgtgg gtggccctga
     4561 acacacagtg ggacacttgc tgt
//
LOCUS       DATBTE010000086         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1239258, whole genome shotgun sequence.
ACCESSION   DATBTE010000086 DATBTE010000000
VERSION     DATBTE010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239258"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..1472
                     /locus_tag="VIG33_03305"
     CDS             228..1472
                     /locus_tag="VIG33_03305"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEY8269888.1"
                     /translation="MQLTLKTILNLKEKHEDFVFRDIKLIDERIEVVVEPRKRSKATC
                     SGCGKRCPGYDRLQQREFIHVPLWGLAVIFLYCMRRVSCVDCGIIVERVPWSTGKSPL
                     TIGYAWYLSEWSKLLSLQEVAKQFKISWHHVFSGVAMAVEWGRERMDLSGITALGVDE
                     IYWAKKSFLTVVYQIDNHCKRLLWCGENRKESTIRSFFDWFGNERSQKLRFICSDMWK
                     PYLKVIAEKATSALNILDRFHIAQKLGEAVDDVRAMETRALKAKGKEVILKHSRWCFL
                     KNPENLTDNQKVKLKDLLACNLKTIRAYLLKEDFQNFWDYTSATWAGKFLDQWCTQAM
                     RSKLEPIKKVAKTIRSHKTLLLNWFEAKNEISLGAVEGQNNKAKVVIRKSFGFKTAEI
                     LKIMLYHKLGKLPVPELAHKYF"
     gene            complement(1876..>2191)
                     /locus_tag="VIG33_03310"
     CDS             complement(1876..>2191)
                     /locus_tag="VIG33_03310"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY8269889.1"
                     /translation="VRVGGTYKMSFTNFSTKNSHSFGGKYIELKPNELLKYIDKFDDP
                     NMPGEMQITVTFRKVICGTELKVTQEGIPEAIPAEMCYLGWQQSLNLLAQLVEPEIPD
                     GN"
BASE COUNT          657 a          421 c          490 g          623 t
ORIGIN      
        1 tggggttcgt ttgaataatt cgtgggttaa aatttaagca aattatgttt tttttgaaaa
       61 gctgaaggtc ttcgctcagc agtgagtgat attgcgaagt ttatccatcc gtgagaacgc
      121 cgaattatgg ccatcgtggc cataatttct aaaaaaagat gcaccttgcg acttcttaga
      181 tgatgatttt tttaccacaa aaaatttcaa tcaagagaat catcaagatg caactcactt
      241 tgaaaactat tttaaatcta aaagaaaagc acgaagattt tgtctttcga gacataaaat
      301 tgatagacga gagaattgag gtggtggttg aacctcgtaa gcgaagcaaa gccacgtgct
      361 ctggatgcgg gaagcgttgc cctggatatg atcgtttgca gcagcgtgag tttatccatg
      421 tccccctctg gggccttgcg gtgatttttc tctattgcat gcgacgagtc tcttgtgtcg
      481 attgcggcat catagtggaa cgggttccat ggtccactgg taaaagcccg ctcacaattg
      541 gctatgcttg gtatttaagt gagtggtcaa agcttttgag tcttcaagaa gtggccaaac
      601 aatttaaaat atcctggcat catgtatttt ctggcgtagc tatggcagta gaatggggcc
      661 gggagcgcat ggatttgtcg ggtatcacgg ccctgggagt tgacgaaatc tattgggcga
      721 aaaagtcatt tttgacagtc gtttatcaaa tcgataatca ctgcaagcgt ctcctctggt
      781 gtggtgagaa tcgcaaagaa tccaccatca gatcattttt tgattggttt gggaatgagc
      841 ggagtcagaa attaagattt atttgcagtg atatgtggaa gccttacttg aaagtcattg
      901 ctgaaaaagc cacgagtgcg ttgaatattt tagatcgttt tcacattgcc caaaagctcg
      961 gggaagcggt cgatgatgtt cgagccatgg agacgagggc cctgaaagcc aaagggaaag
     1021 aggtcatttt aaaacactca cggtggtgtt ttttaaagaa cccggagaac ctaacggata
     1081 accaaaaggt gaagctcaag gatttactgg cttgtaattt aaaaacaatc agagcgtacc
     1141 ttttaaaaga ggacttccaa aacttttggg attatacgtc ggcaacttgg gccggtaaat
     1201 ttttagatca atggtgcacc caagccatga gatcaaagct tgagccgatc aagaaggtcg
     1261 caaagaccat ccgctcacac aagacgcttc tactgaattg gtttgaagca aaaaacgaaa
     1321 tatctctggg ggctgttgag ggacaaaata ataaagcaaa ggtggtgatc agaaaatctt
     1381 ttggatttaa gaccgcagaa atcctaaaaa tcatgcttta tcataagctt gggaaacttc
     1441 cggtcccaga gttagcccac aaatattttt aacgaaccag aaaatcaaac aatgtctctt
     1501 gtggttagca gaatcagaat gaaagcttcc gtctggggca aggacaagga ccgaaaaacc
     1561 tttattcaaa atgttagaca ttgttctaga aaaagttaag agcgaaggtt ttcaaatcgt
     1621 aaccagcaaa tatcatccta ataatcctgc cgtccttatt ccaaagttaa aaagaggttt
     1681 tataatttcc tcaatggaat ttcatgaaag atttcgattc ttaatcgaat taaaattgtt
     1741 ttttgatccc gtgagtcgta gagcttactc taaggcactg ggtttggatc tttaacaacg
     1801 tataaaaaat atttaacgta gggacctgcc aatttcctaa attaaacact cctccaactc
     1861 ataggttatg aattcctagt tcccatccgg aatttcaggc tcaaccaatt gggcaagcag
     1921 attgagcgac tgctgccagc ccaggtaaca catttctgct gggatcgcct ctgggattcc
     1981 ctcctgagtg actttcagtt cagtgccgca gataactttg cgaaacgtga ccgtgatctg
     2041 catttcgccc ggcatattcg ggtcatcgaa tttatcgatg tacttaagca attcatttgg
     2101 tttcagttcg atatatttcc ctccaaaaga atgactattc tttgttgaga aattggtgaa
     2161 cgacatttta taggtaccac ctacccgaac a
//
LOCUS       DATBTE010000087        15280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_282865, whole genome shotgun sequence.
ACCESSION   DATBTE010000087 DATBTE010000000
VERSION     DATBTE010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 15280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15280
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282865"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VIG33_03315"
     CDS             complement(<1..400)
                     /locus_tag="VIG33_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269890.1"
                     /translation="MKIFKSKFIASLACLLFSQSLLATLDSALKSRLSSEMDYIGSIY
                     EQYYAPKNWKETHVAWNLTQEINLAKQGIHDAQSVNDYRQAVVRFFNSMQDYHVGYSF
                     VSTEKASLPILLKTVQGKTLIIEIDRNKLSA"
     gene            563..994
                     /locus_tag="VIG33_03320"
     CDS             563..994
                     /locus_tag="VIG33_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8269891.1"
                     /translation="MAKASTTEVFNCSKEDFFKIISDYEKYHEFLQEVKQCKVLKTEG
                     HRKLVEYSVAVIKSFKYTMWMTEAPPTSIHWEFAGGDMFKTSIGSWKLEDEAGKCRAT
                     YAVEATFNMFVPSPIANALVSVNLPGMMSAYHKRVAQIYRK"
     gene            1006..1401
                     /locus_tag="VIG33_03325"
     CDS             1006..1401
                     /locus_tag="VIG33_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269892.1"
                     /translation="MASDGKHSSKKESEESRVEDGKSLLSDALKKVFTVGISAAFLTE
                     ESIRSYLGEMKLPKDVLHVLLQSASKSKDEITQRMSKEVVAMIQKIDLVKEFSKFTET
                     HKFKITAEVEILKKEPPAKDSPKDMTKKS"
     gene            1709..3076
                     /locus_tag="VIG33_03330"
     CDS             1709..3076
                     /locus_tag="VIG33_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8269893.1"
                     /translation="MFQTINPATGEVIQTYNYLSLKEMDAIVERASRSFRLWREAPLP
                     ERAEVLRKLSVRFREHKKELAALMNQEMGKALEEGVAEIEKCAIACEYFAEMGAEFLM
                     PHPMSAPYALNYPASYAHSEVRFEPLGIIFSIMPWNFPFWQFIRFAAPSIMIGNVILL
                     KHANLTAGCGAKIEELCIGLWDEQIIFNLTLDHSEAARLIQNPKIRGVTFTGSTSGGR
                     EVAKTAGEALKKTVLELGGSDAYIIMADANIEKAAKICAQARMINHGQSCIAAKRFIV
                     HKDVYDNFVLQMKAELSAYPTSVLASGEFQSQLQQQVEKMKKLGGQVLLGGRIPSGVG
                     AFYPPTLIVFDKDDPEIHKEELFGPVALVLRAESVDEAFEIANSSPFGLGGGIFSGNA
                     EKAQDLVAKRMEAGFVMVNDFVKSDPRLPFGGVKDSGYGRELGSFGFYEFCNVKTVVR
                     GISFE"
     gene            3069..4001
                     /gene="tilS"
                     /locus_tag="VIG33_03335"
     CDS             3069..4001
                     /gene="tilS"
                     /locus_tag="VIG33_03335"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY8269894.1"
                     /translation="MNDWLDLEHHLWKELKEHSLLQRKILVGFSGGDDSLSLLWALYR
                     VKKTGMEACYIHHGPGENEKYRDQAEIFCRRFCEERGIPFYTQKNTGLALHSEAELRD
                     FRHSALAAIRLQTNADLLALAHHREDLLETRLLRLIRGTGPQGLSSMRVLQGELFRPF
                     LKISKKDLQEYLKKFSLEAFQDPSNGDFHPLRNWLRHSWLFDLESRQQGAVNSLGRSL
                     EALVEALEENSLPPELFKENYLLRSYYLSLSKTQQKQALASYLLRQGQQNFSQSHLEE
                     IQKRLDNSQKELMFRVGGIDWVINAQQIMVRKSE"
     gene            4085..6043
                     /gene="ftsH"
                     /locus_tag="VIG33_03340"
     CDS             4085..6043
                     /gene="ftsH"
                     /locus_tag="VIG33_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY8269895.1"
                     /translation="MRSTQKTLALWFVLIIMVVFFFHAYENKHQKIIPDFNYSKLVEA
                     VKAGEIASLTFRQETSEILGEVKPEFAKKYNDATKFNIVGNTQDEGYKFFVANGITPN
                     YERADTNNFFQSFVINWLPLLLIVGLFFFIMRQIQAGGGKAMSFGKSRARMLTEHKNR
                     VTFKEVAGVDEAKDDLQEIVSFLKDPKKYTKLGGRIPKGVLLVGSPGTGKTLLARAVA
                     GEAGVPFFTISGSDFVEMFVGVGASRVRDLFEQGKKHAPCLIFIDEIDAVGRHRGAGM
                     GGGHDEREQTLNQLLVEMDGFESNDGVILIAATNRPDVLDPALLRPGRFDRRVVVNKP
                     DLKGREQILAVHMKKTPVGPDVDASKIARGTPGFSGADLENLVNEAALVAARTDKKYL
                     QMEDFEKAKDKVLMGAERKSMVISDEDKKMTAYHEAGHTLVGVLIPGLDPIHKVTIIP
                     RGMALGLTQTLPEKDAVSFSKSQAENWIAFAFGGRAAEELIYKDVTAGAANDIERATA
                     IARRMVCEWGMSEKLGPLAYEKGDGPVFLGMQYGHKTKDYSESKAEEIDREVSRIIGE
                     GYQKALKILTDNKDALERITQALIEYETIDGHEVAMLVNGASVGEIEKVRANKKDDGG
                     LGTAVGGTIAPEKKPAGDPMGNSGPVTI"
     gene            6067..6879
                     /gene="cdaA"
                     /locus_tag="VIG33_03345"
     CDS             6067..6879
                     /gene="cdaA"
                     /locus_tag="VIG33_03345"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEY8269896.1"
                     /translation="MFQQFLDNLIFIVSHLRIQDLIDMILVWMVVYRILVLIKRTGTI
                     QMLSGLGVLAIGYILSIWLELFTFNWILEKFFSNLFVIVVILFQGEIRRALAHIGSNP
                     FFTDVTAAQETHVIEEIAKGIIATAQKGYGALVVVEREIVIDYHIEFGTEIDARVSSE
                     LITSIFHPTSPMHDGALLIRGGKIHSAGCFLPLSKNPALDKNLGTRHRAAIGLTEETD
                     ALVFAVSEENKFISIVQGGHLTANAELGDIRKILYETYGLKYKAFHQGEASL"
     gene            6879..7316
                     /locus_tag="VIG33_03350"
     CDS             6879..7316
                     /locus_tag="VIG33_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269897.1"
                     /translation="MVKKPWTSYVTENFSYKLVALFISLILWLTILGRRDFVLSKKID
                     IEFQTGPNQVIVAQTADNIRVKVSGPRAALKKFVDNPQIQSIVVDISERGEGVLDIDV
                     PLNKIEVPMGVRILGVRPNVIRAEVANKTETKTDKKANPDRSK"
     gene            7326..8702
                     /gene="glmM"
                     /locus_tag="VIG33_03355"
     CDS             7326..8702
                     /gene="glmM"
                     /locus_tag="VIG33_03355"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY8269898.1"
                     /translation="MSEKSIRLFGTDGIRGTANQFPMTPDVVLKVGQAIGYLLQKQVR
                     PGASSVRKVVIGKDTRLSGYMIEQALSSGLNSMGVQVQLVGPLPTPGIGYLTRTMRAD
                     AGIVISASHNAFQDNGIKIFGPDGFKILEDMEKEIEHLVLHEELSRLLPHSREVGRTK
                     RIEDAQGRYIVYVKSTFPLEYTLDGLRIVLDTAHGAAYKVAPAVFSELGAEVIQLGDE
                     PNGININDKVGALYPAKLAEAVIQYRADVGISLDGDADRVIMVDEKGRVMNGDHILAI
                     CALQMKQNGLLKKDTLVATQMSNFGLEKKMTEHGIKVIKVGVGDKYVVEEMRKFGYNL
                     GGEQSGHIIFLDSSTTGDGCVAALSVLGVMKQTGKKASDLNKVVEDVPQVLINCRVKK
                     RIELQEIKGYAELIENIEKKLNGEGRLFVRYSGTEPLLRILVEGPNEIQISEYAEQIA
                     QFLEKELR"
     gene            8703..9434
                     /locus_tag="VIG33_03360"
     CDS             8703..9434
                     /locus_tag="VIG33_03360"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEY8269899.1"
                     /translation="MKNKIRLGVNVDHIATLRQVRGGTTPYPNLLGLVKKSVKGGAEQ
                     ITIHLREDRRHIQLQDLVLLSKKCPVPLNLEMAATEEMAHLAKKYKPQWVCLVPEKRA
                     ELTTEGGLDVVKGFQKMKPLIEKMQMRGVEISLFIEPSLEQVEASYEIGADAVEFHTG
                     HWVLLKGAKKEKEWQRLVNAAHLAHAYGLGVHAGHGLDYHHTRLIKKMPFLREVNIGH
                     SLICYALEEGLEKSVRKMKQILKGR"
     gene            9438..9902
                     /gene="tsaE"
                     /locus_tag="VIG33_03365"
     CDS             9438..9902
                     /gene="tsaE"
                     /locus_tag="VIG33_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEY8269900.1"
                     /translation="MNSLETHFKVFKQYKTTSPKDLEAIVLGLLKLFKERTLLLLEGP
                     VGAGKTELVKTLTQSLGIQETASPSFAIHHHYENRDVSMEHVDLYRLESEDDLESTGF
                     WDLFGVKKGLIVIEWSDRLDQDLLPMNWSKIKIQIENLASPNERQVTVFVQT"
     gene            complement(9899..11209)
                     /locus_tag="VIG33_03370"
     CDS             complement(9899..11209)
                     /locus_tag="VIG33_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269901.1"
                     /translation="MAKLDWQEHFKASIKNVADLYSKDSTNVSLLLFCLRSKLIPVQD
                     YLEWAKENYQLPVIFEKFFEIHKPQLEFYKKWQGRYNWSAECLPIAEWDGTLMVACLQ
                     IPVDYKNSNPTVFVLASHEILNSTWEIYQNPVRTSDTDFIDMSALAATVIATPEQVDF
                     FNADGELALKDPSPAEKFSEELSEELELADLSEMSERDSGSPEGLFGEQRIPSETPIV
                     AASENPKSEKGPRSVQPLMELKIEPVGESAVGLEIDTESVTSISEKNVISPEMNPTTS
                     YLLEKIRQEGKEQFDKDLQANFQQLKTFFKKSMLLAVDDKDCFIKPIFWDGDHFDSQE
                     QQNSEFKLETPSIFKIVNGTQKPYHGFVIVNDLNESFFESWNHGQIPDHITIVPILNG
                     EFIAGMLMGFGEKSCYNKTVLQFTEKVAKDVSQKIFKTSAAKAA"
     gene            11437..12669
                     /locus_tag="VIG33_03375"
     CDS             11437..12669
                     /locus_tag="VIG33_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEY8269902.1"
                     /translation="MDDIRNYYSIKSNYFDHNSTTPVSEDVIQALPDFLQAWGNPSSI
                     HWAGRQPKNILRDARQSLAKNLGCSPLEIVFTSGGSESNNTIIKGLFDYYQTDPLSPS
                     GLKSDLARRKHYMCSEVEHPSVLKTMIYLKSLGARVDLIPVNRQGEMDLQFYQEHLSP
                     ETALVSVMYANNETGTIFPIQKMAEMAHSQGVLFHTDAVQAFGKVPVNLAELGVDFAS
                     FSGHKFYSIKGSGFFFVKKNTPFTSLIHGGGQERHRRGGTENVLGIACLGVMAEKLSQ
                     VSTHVAQVAGLRDHLESRILREIKSVTITAGENPRLPNTSSLVIQGVDGETLLMNLDI
                     KGYAVSTGAACSSGNPEPSPVLLAMGLSRDEAQSSLRLSLGWSTTREQVDDFVEILKP
                     IVERLRLIQAESGGDPHV"
     gene            12662..13765
                     /gene="mnmA"
                     /locus_tag="VIG33_03380"
     CDS             12662..13765
                     /gene="mnmA"
                     /locus_tag="VIG33_03380"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY8269903.1"
                     /translation="MSSESNKKSKGRVLVAMSGGVDSSAAAALLVEQGYDVVGATMQV
                     WDYTSCEIEEGHGTCCSSIDVDDARDVADRLGIPFYVLNCEAKFRAAVIDPFLKTYLE
                     GQTPLPCVNCNTYLKFDHLIRKMKELECDYLATGHYAQIIKDDQGRAHIETSTDDWKD
                     QTYFLFTIDPEIIPKLLFPIGHMKKPEVRAYAEKIGLLNARKKDSVGICFVGAQGYDN
                     FIASQVDKSILMTKKGLLKRYPSGEVMGNHHGIHTFTIGQSKGLGLDHHEKMFVLKID
                     AETNTVWIGEERFLYSSEVKVVDAKLLSEVQEGELMNVKIRYSTKGYPARVFKNEKGF
                     LVKFQDPQRAVTPGQAAVFYREKELVGGGWIVL"
     gene            13750..15096
                     /gene="mtaB"
                     /locus_tag="VIG33_03385"
     CDS             13750..15096
                     /gene="mtaB"
                     /locus_tag="VIG33_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEY8269904.1"
                     /translation="MDRSLNNSVKFQVHTFGCKVNTYDTGLLQKNLQNNGFTSQQGEP
                     RIHVLNTCAVTAEATKEAVRYIRRLKVRDPFCTVVVTGCAAQVDTGAFQDLPGADLVV
                     ANSHKGQLPELLQKYFKGDLTEKVFKSNIFKKEDLEMGGGIEKSHTRTFLKIQDGCNS
                     FCTYCIIPYARGTSRSIPVADLVARVNELYAEGIRECVLTGVHIGDYEDQNSGKKLVM
                     EDLIEALLVKTKMPRFRLSSLEPVELSERLLDIYQDEKMCPHFHMSIQSANTDVLSKM
                     KRKYTQEDVIDSLQRIQKQVKNAFVGMDVIVGFPTETHEQFEDTYLTLAELPWTKIHV
                     FPYSERPGTRAVTYEESVPLQERQRRASRLRELSLHRFETLGRAQEGTHKKVLVLKNT
                     AKGGHSLSRDYWNVEILGANEFLTHWAGQEVDVKILKYDHSNKNHMEGHLIGEILT"
     gene            15093..>15280
                     /locus_tag="VIG33_03390"
     CDS             15093..>15280
                     /locus_tag="VIG33_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEY8269905.1"
                     /translation="MTELETKIRFKFKNSELLARALTHKSFAHELREKGEDNEKLEFL
                     GDAVLDLVLGEYLMELFPG"
BASE COUNT         4159 a         3033 c         3824 g         4264 t
ORIGIN      
        1 tggcagaaag tttatttcta tcgatttcta taataagggt cttgccttga actgttttaa
       61 gtaaaattgg caaagaggct ttctcagtgg aaacgaagga gtaacccaca tggtaatctt
      121 gcattgaatt gaagaaacga acgactgctt ggcgatagtc attcacgctt tgtgcatcgt
      181 ggataccttg ctttgcaaga tttatctctt gggtgagatt ccacgctaca tgtgtttctt
      241 tccagttttt cggggcataa tactgctcat aaatagaacc gatatagtcc atttctgaac
      301 tgagtcttga tttgagtgcg gaatctaatg ttgctagtag cgactgcgaa aagagtaggc
      361 acgctaatga agcaataaat ttacttttga atattttcaa agtttctcct tggtgagatt
      421 tgcgacgaga ctattatctt cttgtttatt ttatgcaagg taaaccaggg aggtgagggc
      481 tttatcctta tcgtaacata atacttgtaa accaactaaa ctcgttattg acactttatg
      541 gcaactccgt aagacttgga ccatggctaa agcatcaaca accgaagttt ttaattgttc
      601 aaaagaagat ttttttaaaa ttatctctga ttacgagaag taccatgaat tccttcaaga
      661 ggtaaaacag tgcaaggttc ttaagaccga aggtcatcgg aaacttgttg agtacagtgt
      721 cgcggtgatt aaatctttta aatacaccat gtggatgact gaagctccac cgacctctat
      781 ccattgggaa tttgccggag gggatatgtt taagacctcc attggttcct ggaagctgga
      841 ggacgaagct ggaaaatgtc gggctactta cgcggtggaa gcgaccttta acatgtttgt
      901 tcccagccct attgccaatg cgctagtcag tgttaattta cctgggatga tgagtgccta
      961 tcataaacgg gtggcgcaaa tttatagaaa ataaggaatt cagttatggc ctcggacgga
     1021 aaacattctt cgaaaaaaga gagtgaagag agcagggttg aagatgggaa atcgcttctg
     1081 agtgatgctc tcaagaaagt tttcaccgta gggatcagtg ccgcatttct cactgaagag
     1141 agtattcgca gttacctcgg tgagatgaag cttcctaaag atgtgcttca tgttctgttg
     1201 caaagtgctt cgaaatccaa ggacgaaatc acccagcgta tgagtaaaga agtcgtggcc
     1261 atgatccaaa aaattgatct ggtgaaggaa ttttccaagt tcaccgaaac tcataagttt
     1321 aaaatcacgg ctgaagtcga gatcctcaaa aaagaaccgc ccgcgaaaga ctcccccaaa
     1381 gacatgacaa agaagtcata actggacttg tctgtttctc attctgggcc gatttgaggg
     1441 gcatgaatac ttatttgctc tcgtttattt cagtcatcct cttttgtctt tgtgcacatc
     1501 agtgcatgaa ggccctgttt cttcctatcc ctgtgtacaa caacacaaag tacaccaagg
     1561 gggcgctcag gatagccaaa ggctataaag ttatttcttg gggagtgatg acagtctgtt
     1621 ttttgctagc ccttgtcatc tcgttttatc aggttttcat agagatctaa gcgcggtaag
     1681 atccttccaa atggaggagg gtgatttaat gtttcagaca atcaatcctg ccacaggcga
     1741 agtgatccaa acatataatt atctaagtct caaagaaatg gatgccattg ttgagagagc
     1801 ttctcgcagc tttcgattgt ggcgagaggc acccctcccc gaaagagctg aagtcctgcg
     1861 taaattatcc gtccgttttc gagagcacaa aaaagaactc gctgccttga tgaatcaaga
     1921 aatgggaaag gctctggagg aaggagtggc tgaaattgag aaatgtgcca ttgcttgtga
     1981 atattttgcg gaaatgggag cggagttttt aatgcctcac ccgatgagtg ctccctatgc
     2041 gttgaattat ccggcgagct atgctcacag tgaagtccgc tttgagccac ttggaattat
     2101 tttttccatc atgccgtgga attttccatt ctggcaattt atccgcttcg ccgccccttc
     2161 aatcatgatt ggcaacgtga ttctcctcaa gcacgccaac ttaacggcgg gatgcggggc
     2221 gaagatcgaa gaactttgca ttggactgtg ggatgaacaa attattttta atttgaccct
     2281 cgatcactct gaggcggcgc gcctcataca aaatccgaaa attcgcggcg tgacctttac
     2341 cggaagtacc agcggggggc gggaagtggc aaagaccgca ggagaggctt tgaagaaaac
     2401 tgtgctcgag cttggtggca gtgatgccta tattattatg gccgacgcga acattgaaaa
     2461 agcggcgaag atttgtgccc aagcacggat gattaaccat gggcaaagct gtatagctgc
     2521 caaaagattt atcgtccata aggatgttta tgataatttt gtcctgcaaa tgaaggcgga
     2581 gctttccgcc tatcccacct ctgtgctcgc cagtggagag ttccaatctc aacttcagca
     2641 acaggttgag aaaatgaaaa aactgggagg acaagtgctt ctcggaggcc gcattccgtc
     2701 gggagtggga gctttttatc ccccgacact catagttttt gacaaggatg atcctgaaat
     2761 tcataaagaa gaactctttg gtcccgtggc tctggtgtta cgagcggaga gtgtggatga
     2821 ggcgtttgaa atcgcaaact catcgccctt tggcctagga ggaggcatct tctccggaaa
     2881 tgctgagaag gctcaagact tggtggccaa aagaatggaa gcaggctttg ttatggtgaa
     2941 tgattttgta aagtccgatc cgcgcttgcc tttcggtgga gtgaaggatt cgggctatgg
     3001 ccgagagctt ggctcttttg gtttttatga gttttgcaat gtaaaaactg tcgttcgagg
     3061 aatttcattt gaatgattgg ttagatctcg agcatcatct ttggaaagaa ttgaaagaac
     3121 acagcctttt gcagcggaaa attcttgtgg gattttccgg gggagacgac tccttgtctc
     3181 ttttgtgggc actttaccga gtcaaaaaaa ccggcatgga agcttgctat attcatcatg
     3241 gtcctggaga aaatgagaaa taccgcgatc aggccgaaat cttctgtcgt cgtttttgcg
     3301 aagagcgcgg aatccctttt tatacgcaaa aaaataccgg gcttgcgctg cactccgaag
     3361 cggagctgcg tgatttccgt cacagtgctt tggccgcaat caggctgcaa acaaatgccg
     3421 acctattggc tctagctcat catcgtgagg atctcttaga gactcgtttg ttgagattga
     3481 ttcgaggaac tggtccccag gggcttagct ccatgagagt tttacaggga gaattgtttc
     3541 gtccattttt aaaaatctcc aaaaaggatt tgcaagaata tcttaaaaaa ttctctttgg
     3601 aagcttttca agatccgtcc aacggggatt ttcatcctct aagaaattgg ctccgccatt
     3661 catggttgtt tgatctagaa agccgtcagc agggggcggt aaatagtcta gggcgctctc
     3721 tagaagcttt agtggaggct cttgaagaaa actcgcttcc cccggagctt tttaaggaaa
     3781 attatcttct aagatcttat tatttatcct tgtctaaaac tcaacaaaag caggctcttg
     3841 cgagctattt gttaagacaa gggcaacaga atttttcgca atctcacctc gaagaaatcc
     3901 aaaaacgact ggacaattcc caaaaagaac tcatgtttag agtcggtgga attgattggg
     3961 tgattaacgc gcagcaaatt atggtccgga agtcagaata agatttttgg atggattatg
     4021 tcttacttgt gaccctaaat cttccatgtt aagctcaaaa catatttctc aagaaggaga
     4081 aagcatgaga tccactcaaa agacgttggc cctgtggttc gttctgatta tcatggtggt
     4141 ttttttcttt catgcttatg aaaataagca tcagaaaata atccctgatt ttaattactc
     4201 aaaattggtt gaggctgtga aagccggtga aatcgccagt ctcactttcc gacaagaaac
     4261 cagtgaaatt ttgggtgaag tgaagcctga gtttgccaaa aaatataatg acgccactaa
     4321 attcaacatc gttgggaata cccaagatga aggctacaag ttcttcgtcg ccaatgggat
     4381 tacgccgaat tatgagaggg cggataccaa caatttcttc caatcctttg tgatcaattg
     4441 gctgccgctt ttgttgatcg tgggattgtt cttttttatc atgcgacaaa tccaagcggg
     4501 cggaggcaag gcgatgtctt tcggcaaaag ccgtgcgcgc atgctgacgg agcataaaaa
     4561 tcgcgtcact ttcaaagaag tcgccggcgt cgatgaagca aaagatgatt tgcaagagat
     4621 cgtcagcttt ttgaaagacc ccaaaaaata tactaaactg ggcggccgta ttcctaaagg
     4681 cgtgctgttg gtgggttcgc cgggaacagg aaaaactttg ctggcacgtg ctgttgcggg
     4741 cgaagcggga gttcctttct ttactatttc cggttctgac tttgtggaaa tgtttgtggg
     4801 ggtcggggcg agtcgtgttc gggacttgtt cgaacaagga aagaagcatg ctccttgctt
     4861 gatttttatc gatgagattg atgctgtagg gcgccatcgt ggagcaggta tgggcggtgg
     4921 tcatgatgag cgtgaacaaa ctctgaatca gttgttagtt gaaatggatg ggtttgagtc
     4981 caatgatggt gttattttga tcgctgcgac caatcgtccc gatgtattgg atcctgcatt
     5041 gttacgtcct ggtcgtttcg atcgtagggt ggtggtgaat aagcctgatc ttaaaggtcg
     5101 tgagcagatc ctggcagttc atatgaagaa aactccggtg ggacccgatg tagatgcttc
     5161 taaaattgcc cgaggaaccc ctggattctc tggggctgat ttggaaaatc tggtgaatga
     5221 agcggctttg gtggcggctc gcacggataa aaaatatctg caaatggaag attttgaaaa
     5281 agccaaagat aaagtcttga tgggtgctga gcgaaaatcc atggtgatct cggatgaaga
     5341 taaaaaaatg acggcttacc atgaggcggg gcataccctg gtgggtgttt taataccagg
     5401 tctggatcca attcacaaag tgaccatcat tccccgggga atggctttgg gtttgactca
     5461 aaccctgccg gaaaaagatg ccgtcagctt ctccaaatct caggctgaaa attggattgc
     5521 ctttgccttt ggtggccgag ccgcggaaga gttgatttat aaagacgtta ctgcgggtgc
     5581 cgccaatgat attgagcggg cgacagccat tgctcgtcgc atggtttgtg agtggggaat
     5641 gagcgaaaag cttggacctt tagcttatga aaaaggcgat ggtccggtct tcttgggcat
     5701 gcaatatggt cacaagacga aagactactc tgagtcgaag gcagaagaaa ttgatcgcga
     5761 agtttctcgg attattggtg aaggctatca aaaggcactg aagattttaa ccgataataa
     5821 agatgctttg gagcgtataa ctcaggctct tattgagtat gaaaccatcg acggacacga
     5881 ggttgcaatg ttagtcaatg gtgcgtccgt tggtgaaatt gaaaaagtcc gcgctaataa
     5941 aaaagatgac gggggtctag gcaccgcagt tggtggcacg atcgcgcctg aaaaaaagcc
     6001 agccggagat ccaatgggca attctggccc tgtgacaatc taactctctc gtgctagagt
     6061 gtgagtgtgt ttcagcagtt tttagataat ttgatattca tcgtatccca cctccgaatc
     6121 caagatctga tagatatgat cttggtttgg atggtggttt atcgtatttt agttctgata
     6181 aaaagaaccg gcacgattca gatgctttca ggcttgggag tgctggcgat tggttacatt
     6241 ctcagtattt ggctcgaact atttaccttt aattggattt tagaaaaatt cttctctaat
     6301 ttatttgtta ttgtggtgat cttattccag ggtgagattc gcagggcttt ggctcatatt
     6361 ggcagcaatc cattttttac cgatgtcact gccgctcagg aaactcatgt cattgaagaa
     6421 attgcgaagg gtattattgc caccgcgcaa aaaggctatg gggccttagt ggtggtggag
     6481 cgtgaaatcg tcattgatta tcatattgaa tttggaacgg aaattgatgc gagagtctct
     6541 tctgaactta tcacctctat ttttcatccg accagcccta tgcatgatgg ggctttgttg
     6601 atccgagggg gtaaaattca ttctgcgggt tgttttttgc cactgagtaa aaatcctgcg
     6661 ttggataaaa atcttggcac tcgtcatcgc gccgccattg gcctgacgga ggagacggat
     6721 gctttggtgt ttgcagtgag cgaggaaaat aagtttatca gcattgtgca agggggacac
     6781 ctcaccgcca atgcggagct aggtgacatc cgtaagattc tttatgaaac ttatggtctc
     6841 aaatacaagg ccttccacca aggggaggcc agcttataat ggtcaagaaa ccgtggactt
     6901 catacgtgac ggagaatttt agctacaagc tggtggcgct ttttatttcg ttaattctgt
     6961 ggctgacgat tttaggtcgt cgcgactttg tattaagtaa aaagatcgat attgaatttc
     7021 agaccggtcc gaatcaagtc attgtggcgc aaaccgcgga taatattcgg gtgaaagttt
     7081 ctgggccccg ggccgctctt aaaaaatttg tggacaatcc gcaaatccag agtatcgtcg
     7141 tcgacatttc ggagcggggc gaaggagttt tggatatcga tgttcctctt aataaaatag
     7201 aagttcccat gggtgttcgg attttgggag tccgtccgaa tgtgatccgt gctgaggtgg
     7261 cgaacaagac tgaaactaaa accgacaaaa aagcgaaccc tgatagaagt aaataggaaa
     7321 acaaaatgtc agaaaaatcg attcgtttat tcggaactga tggaattcgc ggcactgcca
     7381 atcagtttcc catgactcct gatgtggtgc ttaaggtggg gcaggcgatt ggctatcttt
     7441 tgcaaaaaca ggtgcgtcct ggggcttctt ccgtgcgtaa agtggtgatc ggcaaagaca
     7501 cccggctctc tggctatatg attgaacagg ctttgtcctc aggcttgaac tccatgggag
     7561 ttcaagttca gttggtgggc cctcttccta cccctggaat cggttatctg acccgcacca
     7621 tgcgggcgga tgcggggatt gtgatttccg cttctcataa tgcttttcaa gacaatggaa
     7681 ttaaaatttt cggtccggat ggctttaaga ttctcgagga tatggagaaa gaaattgaac
     7741 atttggtttt acacgaagaa ctcagccgct tgctgcccca ttcccgtgaa gtcggccgga
     7801 ctaaacgcat tgaagatgct caaggacgtt atattgttta cgtgaaatcc acctttcctt
     7861 tggaatacac tttagatgga ttgcgtattg ttttggatac agcccacggt gcggcttaca
     7921 aggtcgctcc cgctgttttc tccgaactcg gtgcggaagt gattcagttg ggcgatgaac
     7981 ccaatggaat taatatcaat gacaaagtcg gagcacttta tccggcaaag ttggcggagg
     8041 ctgtgattca ataccgtgct gatgtgggga tcagtctcga tggcgacgcg gaccgggtca
     8101 ttatggtgga tgaaaaaggc cgggtgatga atggcgacca catcctggcg atttgcgctc
     8161 tgcaaatgaa gcaaaatgga cttcttaaaa aagacactct ggtggccaca caaatgagta
     8221 acttcggact tgaaaagaaa atgaccgagc acggaatcaa ggtcatcaaa gtcggagtcg
     8281 gtgataaata cgtcgtggaa gagatgcgta agtttggcta taacctaggt ggtgagcagt
     8341 ccgggcatat tattttcttg gactcttcca cgaccggaga tgggtgtgtc gccgctctca
     8401 gtgttttggg tgtaatgaag caaaccggca agaaagccag tgacctcaat aaagttgtgg
     8461 aagatgtgcc tcaggtcctt attaactgcc gagttaaaaa acgcatcgaa cttcaggaaa
     8521 tcaagggata tgcggaactc attgaaaata tagaaaagaa attaaatggg gaaggccgtt
     8581 tgttcgtccg ctattctggc accgagccgc ttttaaggat tttggtggaa ggaccgaacg
     8641 aaattcaaat cagtgagtat gctgagcaaa tagctcaatt tttagaaaaa gagttgagat
     8701 aagtgaaaaa taaaattcgt cttggtgtga atgtggatca tattgcaact cttcgtcagg
     8761 ttcgaggagg caccaccccg tatccgaatc tcttgggttt agttaaaaaa tcggtgaaag
     8821 gtggagcgga gcaaatcacc attcacttac gggaagaccg tcgtcatatt cagcttcagg
     8881 accttgtctt gctttctaaa aagtgtcctg ttcctttaaa tcttgagatg gccgccactg
     8941 aagaaatggc gcatttagca aaaaaatata agccgcaatg ggtgtgcctg gtgcctgaaa
     9001 agcgcgccga attaaccacg gaagggggac ttgacgtagt caaaggcttt caaaaaatga
     9061 aacccttgat tgagaaaatg caaatgcgag gtgttgaaat ctccctgttt attgagccgt
     9121 ctcttgagca agtggaagca agttatgaga tcggcgcgga cgcggttgaa tttcatacgg
     9181 gacattgggt gttgttgaaa ggtgctaaaa aagaaaaaga gtggcaacgc ttggtaaatg
     9241 cggctcattt agcccatgct tatggcttag gcgtgcatgc ggggcatggt ttggattatc
     9301 accacactcg tttgataaaa aaaatgccct tcctgcgaga agtgaacatt gggcattctt
     9361 tgatttgtta tgctctcgaa gaaggtcttg aaaagtcagt ccgcaaaatg aagcaaattc
     9421 tcaaaggacg ctaagctgtg aatagcctgg agactcattt taaagttttt aaacagtata
     9481 agaccacgtc tccaaaagat cttgaggcca tcgtccttgg actgctgaag ctctttaaag
     9541 agcgcacgct attgcttcta gaaggacctg tgggggctgg aaagacagag ttggttaaga
     9601 cgctgaccca gagccttgga atccaggaga cggcctcgcc ctcttttgcg attcatcatc
     9661 attatgaaaa tagagatgtt agcatggagc atgtggatct ttatcgtctt gaaagtgagg
     9721 atgatcttga gagcacaggt ttctgggatc tttttggtgt taaaaaaggc ctgatcgtga
     9781 tcgagtggtc agatcgtttg gatcaagatt tactgcccat gaattggtcg aagataaaaa
     9841 ttcaaattga aaatctagcc agtcccaacg aacgccaagt gacggtattt gttcaaactt
     9901 aagccgcttt ggcagctgat gttttaaata ttttttgaga aacatcttta gccactttct
     9961 cagtgaattg caggacggtt ttgttgtagc atgatttttc cccaaagccc attagcattc
    10021 cggctatgaa ttcgccgttt aaaataggca ctatggttat atgatcgggg atttgaccat
    10081 gattccaaga ctcaaaaaaa gactcattta aatcatttac tatcacaaat ccatggtagg
    10141 gcttctgggt gccgttaacg atcttgaaaa tactgggggt ttctaattta aactctgagt
    10201 tttgctgttc ctgagagtca aagtggtccc catcccaaaa tataggtttt ataaaacaat
    10261 ctttatcatc aacggccaga agcatcgatt ttttaaagaa agtctttagc tgctggaagt
    10321 tggcttgaag gtctttgtca aattgctctt ttccttcttg tcgaattttt tcgagtaagt
    10381 aggatgtcgt agggttcatc tcgggagaaa ttacattttt ctctgatatt gaagtaaccg
    10441 attcagtatc tatttctaag ccgacggcgg attcacccac tggctctatc ttaagctcca
    10501 ttaatggttg aacactgcgc ggtccttttt ctgactttgg attctcgctt gcagcaacta
    10561 ttggagtttc gctcggaatc cgctgttcgc cgaaaaggcc ttccggagag ccgctgtccc
    10621 tttcactcat ttcactaagg tcagcgagct caagttcttc cgaaagttcc tcggagaatt
    10681 tctctgctgg tgatggatct tttaaggcca gttctccatc agcattaaaa aaatcaactt
    10741 gctcgggggt ggcaatgaca gtggcggcaa gagcggacat gtcgataaaa tctgtatcac
    10801 tggtacgcac ggggttttgg taaatttccc aggtactatt taagatctca tgggaagcca
    10861 aaacaaagac cgtgggattt gaatttttgt aatccacggg tatttgcaga caggccacca
    10921 tcaaagttcc atcccactcg gcaatgggga ggcattccgc agaccagtta tagcgtccct
    10981 gccatttctt gtaaaattca agctgaggct tgtggatttc aaaaaatttt tcaaaaataa
    11041 cggggagttg ataattttct ttcgcccact cgagataatc ctgtactgga attaactttg
    11101 aacgcagaca gaagagtaag agcgatacat tggttgagtc tttagagtaa agatccgcca
    11161 catttttaat cgaggcttta aaatgttcct gccagtctaa ttttgccatt cttcccttta
    11221 tcggtctaag tcatgaactt ccttatgcct ttttcactaa aagtaccgac gaaggcgtct
    11281 agttttgttt attgaggagg gggttttgta ttttttactg tctaaaacaa cgacaagaag
    11341 cagaagaata cggcaataat ctgtttccag gcgttgctga gaccccaatc ttaaatgttt
    11401 tcagtaactt ccctttgtga taaggtgcgg cactctatgg acgatatccg caactattac
    11461 tcaatcaaat cgaactactt cgatcataat tccaccactc cggtgagtga agacgtgatc
    11521 caagcactcc ctgatttttt acaagcttgg gggaatccta gttcgataca ctgggcgggc
    11581 cgtcagccta aaaatattct gcgcgatgcc cgacaaagtc tcgctaaaaa tctaggttgc
    11641 agtcctttgg aaatcgtatt tacctccggc ggttcggaat cgaataatac tatcatcaaa
    11701 ggcctctttg attattatca aacagatccc ctttccccat ctggtttaaa atcagattta
    11761 gctcgccgca agcactatat gtgttctgaa gttgagcatc cgagcgtgct gaaaacgatg
    11821 atttatttaa aatccttagg ggcacgggtg gaccttattc cggtgaatcg ccaaggggaa
    11881 atggatcttc agttttatca agaacatctc agtccagaga cagcattggt ttctgtgatg
    11941 tatgcaaata atgaaacagg gacgattttt cccattcaaa aaatggctga gatggctcat
    12001 tcacaagggg tccttttcca taccgatgca gttcaagctt ttggaaaagt tcctgtgaat
    12061 ttggcggaac taggagtcga ttttgcttcc ttttcaggtc ataaattcta ctctattaaa
    12121 ggttctggtt ttttctttgt aaagaaaaat actcctttca cttctttaat tcatggaggc
    12181 gggcaagagc ggcatcgccg tggcggtacg gaaaatgtgc tgggaatcgc ttgtcttgga
    12241 gtgatggcgg agaaactctc tcaggtgtcc actcatgtcg ctcaggtggc aggtcttcgg
    12301 gatcatctgg agagtcgtat tctgcgggag atcaaaagtg tcaccatcac tgcgggggaa
    12361 aacccgcgct tgcctaatac ttcgagtttg gttattcagg gcgtggatgg agaaaccctg
    12421 ctgatgaatt tggatattaa gggttatgca gtgagtacgg gggcggcttg cagcagcgga
    12481 aatccggaac ctagtcctgt cttgttagct atgggattga gtcgggacga ggcgcagagc
    12541 agtttgcgtc ttagtttggg ctggtccact acgcgagagc aagtcgatga tttcgtggaa
    12601 attttgaaac ccatagttga aaggcttcgt cttattcagg cggagtcagg gggggatcct
    12661 catgtctagt gagtccaata aaaaatctaa aggtcgcgtt ctcgtagcca tgagcggggg
    12721 agtcgatagc tccgcagcgg ccgctctctt ggtggagcag ggttatgatg tggtcggtgc
    12781 cactatgcag gtctgggact atacctcttg tgagatcgaa gaaggtcacg gaacctgttg
    12841 ttcgagtatt gatgtggatg atgcccgtga tgtggcggac cggctcggga ttccttttta
    12901 tgttttgaat tgtgaagcga agtttcgcgc ggcggtgatt gatcccttct taaaaactta
    12961 tttagaaggt caaacgccac ttccctgcgt taattgcaat acgtatttaa aatttgacca
    13021 tttaatcagg aaaatgaaag agctcgagtg tgattacctg gcaacgggac actatgctca
    13081 aatcatcaaa gatgatcagg gacgtgcgca tattgaaacc agtacggacg actggaagga
    13141 tcaaacttat tttttattta cgattgatcc cgaaataatt ccaaagcttt tattccccat
    13201 cggacatatg aaaaaaccag aggttcgggc ctacgcggaa aaaataggtc ttttgaacgc
    13261 ccgaaagaaa gattccgtag gaatctgctt tgtgggagct cagggttacg acaatttcat
    13321 tgcttcgcaa gtggataaaa gcattctcat gactaaaaaa ggtttattga aacgctatcc
    13381 atcgggtgaa gtgatgggaa atcaccacgg cattcatacc ttcactattg ggcaaagcaa
    13441 gggtctggga ttggatcatc acgagaaaat gtttgtttta aaaattgatg ctgagaccaa
    13501 cacggtttgg attggcgaag agcgttttct ctattcaagt gaagtcaagg ttgttgacgc
    13561 taagttactg tctgaggttc aggagggtga actgatgaat gtaaaaatac gttattcgac
    13621 aaaaggctac ccggcgcggg tgtttaagaa tgaaaaaggt ttcctggtga agtttcaaga
    13681 tccccaaagg gccgttacac caggccaagc cgccgtattt taccgtgaaa aagaattggt
    13741 ggggggcgga tggatcgttc tttaaataac tctgtaaaat tccaagtcca tactttcggt
    13801 tgtaaagtga acacttacga tacgggactt ttgcaaaaaa atctgcaaaa caatggtttt
    13861 acatctcagc agggagagcc tcgtattcac gttttgaaca cctgtgcggt gactgcggaa
    13921 gcgaccaaag aggcggttcg ttatattcgt cgtttgaagg tgcgagatcc tttttgcacg
    13981 gttgtggtca caggctgtgc ggcgcaggtg gatacagggg ctttccagga tcttccaggg
    14041 gccgacttgg tggttgcgaa ctcccacaaa ggccagctgc cagagctgct tcagaaatat
    14101 tttaaaggcg atcttacgga gaaagttttt aagtccaata tctttaaaaa agaagatctt
    14161 gaaatgggtg gaggcattga aaaaagccac accagaacat tcttaaaaat ccaagatggc
    14221 tgcaacagtt tttgcaccta ttgcattatt ccttatgctc gcggcacctc ccgcagtatt
    14281 cccgtagctg atcttgtggc ccgggtgaat gagctttatg ccgaagggat tcgcgagtgt
    14341 gttttaaccg gtgtgcatat cggggactat gaagatcaaa acagtggcaa aaaactggtt
    14401 atggaagatc ttattgaggc tttgctggtg aaaaccaaaa tgccacgatt cagactttcc
    14461 tcgttggaac cggtggagct gagtgaaaga cttttagata tttatcaaga tgaaaagatg
    14521 tgcccgcatt ttcatatgag catccaaagc gccaatacgg atgtgctcag taaaatgaag
    14581 agaaagtaca cccaagagga cgtgatcgat tctctgcaga gaatccaaaa acaagtgaag
    14641 aatgcatttg tcggaatgga tgtcattgtg ggttttccca cggaaaccca tgagcagttt
    14701 gaagatactt acttaactct tgctgaactc ccttggacga aaattcatgt ctttccttac
    14761 agtgagcgtc ccggcactcg tgcggtgact tatgaagaat cggtgccgct tcaagaacgt
    14821 cagcgtcggg cttcacgctt gcgcgaacta agtcttcatc gctttgaaac tttaggcagg
    14881 gcacaagagg gaactcataa gaaagtcttg gttttaaaaa ataccgctaa aggcggtcat
    14941 tctttaagcc gtgattattg gaatgtcgaa atcctgggag ccaatgaatt tctgactcac
    15001 tgggcgggac aagaggtgga tgtgaaaatc ctcaaatacg atcattctaa taaaaaccac
    15061 atggaaggtc atttaatagg ggagattctc acgtgaccga gttagagact aagatacgat
    15121 ttaaatttaa aaatagcgaa ttgttggctc gcgccctgac tcacaagagc tttgcccatg
    15181 agctgcgtga aaaaggtgag gacaatgaaa aactcgagtt tttgggcgac gccgttttgg
    15241 atctggtttt gggcgaatat ttgatggagc ttttcccggg
//
LOCUS       DATBTE010000088        32730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_146370, whole genome shotgun sequence.
ACCESSION   DATBTE010000088 DATBTE010000000
VERSION     DATBTE010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 32730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32730
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146370"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VIG33_03395"
     CDS             complement(<1..554)
                     /locus_tag="VIG33_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269906.1"
                     /translation="MSDLKTKLFSRKKTWFAEAYETTEEAFLVAEYFARYAKKRWPFA
                     SETTDRNPRGYLYLEEIDVVFCSLDKVPRKVLKLGYGSSVLQVLLPNLGYTEEQKEIL
                     RDFYQRKYAPILKESHARTNALLWHTFSEAERREILRAFRANSQTDLKIKKDVLRDIT
                     KIIAGNESEIIKMQTRTSTHKKVR"
     gene            719..1132
                     /locus_tag="VIG33_03400"
     CDS             719..1132
                     /locus_tag="VIG33_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269907.1"
                     /translation="MAIELGTSDIRKTFMGLVRPHLNNKEKKYEAKKFTGLELGTLDG
                     MIYDGRGGIEAWQKLLTYVFKISDNEIDQVLLEFKDYLKARIKMTPGELMWAQLGEKL
                     TEDEKIFFCELAKAHKMLKPPFEIKLTKKTRKSLR"
     gene            1603..4563
                     /locus_tag="VIG33_03405"
     CDS             1603..4563
                     /locus_tag="VIG33_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8269908.1"
                     /translation="MSDLKSDSIRTSKTAQKNKLQNTRERTPVIENFEARPVILAVVA
                     SQSIADSICAELGSEYRIETAANGREGLKKTLQIHPDLILTDLMMPIVNGEQFFHAIR
                     SHAEIKSIPFLILAAKGEEELRLKLLREGADEYLIKPFMPEELRVRVKNLVRHVQDQK
                     SLAHELLVREQVEEIFRRQEFQLKEAQRITNFGSWDWDIVKNKLTWSDESYRIFGYEP
                     QQINPTYEALLSRVHPDDLDRVNEAVHKVLFEKKPYSLQHRLVWPDGSERIIVSVAEV
                     QFSSEGRPLGMIGTIYDISEQEEVKRRLEEAELKYSGLFESAADAIVVVDNHGRIESV
                     NQQTVKWFGYSRQELLGQPVEILIPERFKQTHILERNRYLTQPSHQIMGSGREIVAKR
                     KDGGEVPIEVSLTPLGSGKNKVVAAIIRDITDRKKIEMQQNFFSESGRIILETMDPQV
                     RLDRGANMMIPKIADICLVSTYEQKDFHFSALVHYDPAKQKLLEELTAKDCSWRNFPF
                     NAKSVSELQEPILIEDVAEGALENSVAIRNMNQLMKRMNVKSYIILPMVVRERRIGSM
                     TLAMSDSGRKFRHDDFAFLKEVALRFAVAADNAKLYWDSQQAIKDRENVLFIVSHDLK
                     NPLSSIKLAGEFLEEYHLPTEQDFFLVAKKILQSAEIIERLIMDWLDFGKVQSGTLVI
                     RKKSESLADIIESSLEAMKVKALERNIKLVVDLPKTDVMISCEKNRILQVLWNLLGNA
                     IKFTSENGEVRIKAMKSDNSVQISIIDTGQGISPEQIPRIFDRFWQATETAKLGTGLG
                     LSIAKGIIEAHGGKIWVESKVGKGSQFHFTIATADEVKSPTTPNHAHDHKFHCEYLEG
                     KRLKGIRVLLVDDSPEVLFMMRFILEKAGAEVSQARSMREALTSLKQEKPHVMLSDIE
                     MPDGDGFELLRRVQQLSVEEGGRIPAAALTGHREEDKLKQMKEAGFQLQLQKPVTCEE
                     LVKAVSELAGKLSL"
     gene            complement(4594..5478)
                     /locus_tag="VIG33_03410"
     CDS             complement(4594..5478)
                     /locus_tag="VIG33_03410"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEY8269909.1"
                     /translation="MSLDFLKGACRFRDIGGKFKESGQTFLTVKTVDLSIAWRHLLKR
                     KILMDIKIENAHLLIPKDLKKLSFPKKETQDITDTFFPLKIEQVDLQDSSITFEKYKS
                     LKENKPLKISAINGRILNLTPSPQIPLSSFNVTAKLIDPNSKFKFGGKFNRLATPIPW
                     SVDIEVEDFDIVTLDPYLKSHVPLTFTKGTLDLYSEVLSKEGQVRGYFKPLLKDIDVV
                     SNKEKFIGAKHFGIEMITALVNLILRDKKTKSIATIIDFKYDKKLSFNIKKGLSKAAK
                     QGSENEVRPGFENRYRLQ"
     gene            complement(5831..6430)
                     /locus_tag="VIG33_03415"
     CDS             complement(5831..6430)
                     /locus_tag="VIG33_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269910.1"
                     /translation="MKYLGSPLFTVFLVFLLSSCASSTVNKNGLNIMSESDYQATVDK
                     WTDYIEEYNGLNNTVSIKATLLNPEMATAQLDQNARIFQWDHNTFDTEKKKLENNLNQ
                     QTEVFVSFFSPERKWDDLHKSKTLWKVFLDVNGQRFEGKATKIKLLPREIQSLYHYHT
                     SFASPYIISFPVSARTVDNKPARLTFTGAIGSASLNFGK"
     gene            complement(6427..7287)
                     /locus_tag="VIG33_03420"
     CDS             complement(6427..7287)
                     /locus_tag="VIG33_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HEY8269911.1"
                     /translation="MTQNKYKNLEGHFIGAHNANLFFQLWENPRARGTIVITHGHGEH
                     SDSYKRVVEALKDDVWNIYAWDMRGHGKSEGARGYASSFDDYCSDYVEFLKMVFAQPL
                     ITKGPVVLLAHSMGGLVQIKALLQHPEFKPDAVVLSSPLFGIGVEIPAYKKLGAEWIA
                     KLLPTLTMWNEIGNQDVTRDPEVIRELEHDPYRHARVCAAVYLGFLESFKFVYEGIPQ
                     LKVKTLFQISDKDPVISSAEAMRFFEHFGSLEKEIKIYKDAKHEIYNDIIREQAFEDL
                     RHFLAGVEKK"
     gene            complement(7318..8973)
                     /gene="nadB"
                     /locus_tag="VIG33_03425"
     CDS             complement(7318..8973)
                     /gene="nadB"
                     /locus_tag="VIG33_03425"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEY8269912.1"
                     /translation="MRRCDVLIIGSGLAGLSLALKMASSGKVVILSKERALDSNTDMA
                     QGGIAAVMSQEDSFESHIQDTLTAGAGLCKPEVVANFIEQAPDRIQDLLDWGVHFDLR
                     RGVDNEENSIDLTREGGHSYRRILHFEDQTGHEVHQTLLRRIRENPNIELLENHFAID
                     LIINKQVDTYDMSPTRCVGAYSLNKKTGEVAAFLGKSTVLATGGAGKVYLYTSNWSGA
                     TGDGIAMAYRAGARIANLEFMQFHPTCLYHPAARNFLISEALRGEGGELINSQGEAFM
                     RKYHSLGSLAPRDIVARSIDAEMKKSGAHCVYLDMTKLDAEFLRKRFPVIYNKCLEYG
                     IDLTKTPIPVVPAAHYLCGGVLTDIQGQTDIPGLRAIGETACTGLHGANRLASNSLLE
                     CLCMAHNASESLKQQWAALEICPHNPKDWHYPEESNDDEMIVISHMWEEIRHLMWNYV
                     GIVRSNKRLQRAQHRLRNILSEVREYYSHFRIHSDILELRNIAIVAELSVECALRRKE
                     SRGIHYNLDYPFVPKVIALTKTEEDKRGTDPQAKDTILLRGLR"
     gene            9171..11207
                     /locus_tag="VIG33_03430"
     CDS             9171..11207
                     /locus_tag="VIG33_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8269913.1"
                     /translation="MKLKLLITILVLATLFSGVVLWRVDRFVYGDRMAWAEAQARTQL
                     SSLSQAINTEIQSSRRMLSSVSNDTFKRESTNWKAFQPYYAIALMTSQSGALSISRLV
                     SKADSPAANWTVNDLTQSIGFMGRGLENRGTILLRSFKDAQKNRHVAVIFAGGGNAYL
                     LIGRGENFQSLIESQKGSMSAISIIANDGLTISHAVPDYIGNVMSESTLLKEIRTTGA
                     AQGLGVYLQGKRPVFGMYSQIPGTNSYVISSVPLEDLLKGRLSLAWQFGFMAVGILLL
                     GAALYFWQEKRVVSAALPKRPQSVKTTPRPLEYEAPVALKQPIVATPQELQQEKTDAY
                     RQVATALGQEMRSPLASILGFSQMVLSKTQDPDVVQAVESILREARSSRDVVEKLVTF
                     AGERSTDKTNSKIEGPIIQALKNLDLKLSQKGIRVEKDFKETPPWPLAFADVTKAFEN
                     LFINAIEAMERMQEKTLRISSWESAEGLHVKIADSGEGIDVENVAKVFDPFFTTRSFA
                     HHVGLGLPVVVGILKEHNGKIQIHSQPGKGTQVEIIFKANSVAGTGTSLPKVPPPPPA
                     KFEMPTDLPKAPEISLEAEIPKVEVFKKQISQEKLTDINVDNLLELTEDEEPLRFLEG
                     HGFENSVPSAPLPQTPEPKTVDPAVVLDMRVAVPLKKTELDSYKVEIRRPGKRT"
     gene            11208..12689
                     /locus_tag="VIG33_03435"
     CDS             11208..12689
                     /locus_tag="VIG33_03435"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8269914.1"
                     /translation="MNIRDHSSQFSIFTYSTDVDQGALVKVSLAQAGYDAYYFQELDI
                     FLSRLKEMTPHVVVFSTMTVQGSLSEFVNQVLEVSSEIKLIAMAELPQFESLSRYSTT
                     GLADIVSLEGPSLDLRVLWSVDQACEALYLTYQNEQLYADWKEAQGLKEKLQNQMDDQ
                     KAMQAEVTQMLLSNRVADYRSSQNKEEVLQKYFERLEKVPAIYFKYLSSVRSFVATHV
                     SGFSPQQIQGVGCQLEDPSLDNLSSQVSVGLLPPVMMEMVQKVFAIENPRVLPLFVQG
                     ELEGLVVYSGDLGKADSLRVGEEFSLFALCYSYFCLEKKVEALDVMDPVTELYNEKFY
                     VDKLHEELERSRRLRQPVSVLKISIDSFSEIEQVLGSNACNGVLKNLGDFVKRTLRIN
                     DFACRTKANEIGVILPHCHRKGAALLGERLRRIVESSSMVDSGVKISISIGVSEYPSL
                     ADSVDTLDGTASKALVHIMEKGGNRICLYKPPESFTPEFEVVG"
     gene            12721..13941
                     /gene="alr"
                     /locus_tag="VIG33_03440"
     CDS             12721..13941
                     /gene="alr"
                     /locus_tag="VIG33_03440"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEY8269915.1"
                     /translation="MYRRTLAEINLDHVAHNFEVLKKAFSHAPFICPMVKANAYGHGD
                     VAVALCLEKVGAQHLGVCLIEEGLLLRRGGVKAEILVFRGFDREGAKTMLQHKLTPVV
                     ATWDQLHALQSVASQAKQPVQIHLKFDTGMNRLGFPVAEAQRLFELCWQNSKVRVQGI
                     LTHLCHGEDAQDPAGDSAAQLSSLAQVMKIFKPLNPVVHSLNSAGILNSLIMARRQKD
                     PHHPLSRVQWGLRPGLMIYGYSPLAQKDILPLKPVMTLKSVASTYRVLKAGEKVSYGG
                     TWTASKASVIAVVPIGYADGYHRILSNKGQALFLGQRVPVVGRICMDFLMVDVTEVVL
                     HKDLKELEEESIILFGQDRNGNCLSPEELALQAQTITWEILTSVGERVPRVYTGDMAK
                     KLVKQLAKQRGDAD"
     gene            13938..14765
                     /locus_tag="VIG33_03445"
     CDS             13938..14765
                     /locus_tag="VIG33_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8269916.1"
                     /translation="MSLVQKMSQIIDLILNYFRGKIGGFVEEVGKIVLFMTSSIRLLF
                     AKPYRYTEMIRHMEFVGNQSIGIICLTGLFTGLALSLQIYLGFKMFNAVNMVGPVVAL
                     GITRELGPVLTGLIVAARAGGAMAARLGTMRVNEQIDALDVMGVNNKQYLIAPRIAAA
                     VICMPLLTAVFDFVSMLGSYLLCVKLVQIDEAVFWQKIRDMIQVRHINEGLFKAAIFG
                     LIFAVICTYRGFYTTGGAKGVGEATNQGVVQSMVMIIILDYFAGNLIRLFYLVMGMN"
     gene            14765..15550
                     /locus_tag="VIG33_03450"
     CDS             14765..15550
                     /locus_tag="VIG33_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8269917.1"
                     /translation="MEDHSKIPSFTASAVSLRDVKKTFDGNEYILKGMSLEVPQGSLT
                     AIIGFSGTGKSVVLKHILGLYQPSAGEIHVLGVNIGLLPPNELTRFRQRFGVLFQSAA
                     LFDDMTVLENVCFPLVEHRRDLRSAQVIHLAEQRLDQVGLDKKHYHKLPNQISGGMQK
                     RTGLARALALDPEILIYDEPTTGLDPILTEMVDNLILDTHKSRKGLTSIMVSHDLAAA
                     FRIADYVVMLDAGKVLLSGTAKDFFNSNIELVQRFVAKGMKHS"
     gene            15547..16935
                     /locus_tag="VIG33_03455"
     CDS             15547..16935
                     /locus_tag="VIG33_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY8269918.1"
                     /translation="MNWVKSPEFKVGLLVVVVGSLIAFMSMQVSEDPSYLGRSKKAWF
                     LLKNADGLVKDSAIRTAGIPIGIIRDIILQDGQARVDITVKSEVHLTVSAVVEIRAQG
                     ILGDKHVEINPGVPSDPPLPEGAQILNVKEKGSLDNLVGEVSEISSSLKTVSETLKEA
                     ITEDGTRKHILGRIVSNIEKLTGDIAQVTGENKEKIGEIVDQVHDVTSTLSNLVNDEG
                     EDGFKKSWKKAVASLSRLDSIMKNVDEISGKINRGEGTIGKLVNDESTVEGLNTTIEG
                     ISGMMETANRIQTGFDFHAEYLGEVKATKSTVGIYIQPGLDRYYYLGIVDDPAGVIER
                     EGSVSTTNGVVTTTDQTKTFYNKTKFSLLFAKNFYNLTLRGGLIENQGGIGIDYLFFR
                     RKLKLSLEAFDFSKLNLRAQAQYSLSHGIYVLGGMTDMMDKSDRRSGYLGAGIYITND
                     DLKLLLSSKSPF"
     gene            16960..18741
                     /locus_tag="VIG33_03460"
     CDS             16960..18741
                     /locus_tag="VIG33_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8269919.1"
                     /translation="MAAGPENNHLTRNWLVKSSTKILGPFTREEVMSLLTRRQITIID
                     EVRQPEGRWTYIRENRHFKEVVKSLRYEQDHSKEDTMTSTATLGATMTKTDPATVSDE
                     FTPTPITPMKSPPLHIQKPGLKDVTPLKETSLRPQGGAGAAKSFGTLSDQRVQSKIQK
                     QNRLLRGILFVITGVAVLFATLHFIFKEKKSDINYDQLLRSALSYKEIGLYQLSLQKY
                     KKAFSIKEPDLESQFQMVFLLINEDRQSLNGRRVIERALQTEGRNRVEIINAYLGMAL
                     SYMMEGDLRQAEEYLQKTLGYDANNENAKINQAVILLKKGNYAQALKSFEALSKVDSP
                     VYPLVLLCESLSLIEVSRGQANKDRLLKAVSEIKSYSAKSHFLRKELSLLMLYLSRLG
                     GDPEGEQDAINTFLEEPQNLSRQYARELQVDWRNSEWDFLERYCAELYNSTKGPANLK
                     AVRAVCLIESNRDMEAVKYLDESMAQAPKELTTLQAQAQYLWKLGRLKEAQLLFQNTD
                     FKQDRLALYIQGEACLQAKDLGCAEAAYKILSERDYGDVLAHYGLALLTQARNDKVRL
                     QNEIKAGFEAESNFAPLIEIRDQMESM"
     gene            18738..19403
                     /locus_tag="VIG33_03465"
     CDS             18738..19403
                     /locus_tag="VIG33_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269920.1"
                     /translation="MKKGSLLFTLSIFSGLMACTKPGSFRSEDPQAVKMVKVDEIPED
                     FKIPSNIWELIAKNGDEKSVEVNKGGVFYSTVKLFLTEKNSHILQNPSFVIELPRGGG
                     IVDMSRYLSGQQGSFYVGFELPAEFQEGKNFKAIYISKARKSKLDDRVFGAGCNKYFD
                     ITVKFLEMMKTEGIKANTTRQRHVTLLAGHYIFSVIKDNRIHVTQVTITDSQNKNLLC
                     EAL"
     gene            19400..20365
                     /locus_tag="VIG33_03470"
     CDS             19400..20365
                     /locus_tag="VIG33_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY8269921.1"
                     /translation="MMEKKTVRELKDKDAVDSLFLVKEKNVNVGKNGRPFMSLVFGDQ
                     TGSIDGRVWDRVEELSQEFEVSDILRVKGQIQIFQNRKQLIVHRLEKPDQSALNVEDF
                     IPTSGKNTDDMFMELLVLVRSMKNDHLRLLTLDVIEDAEIKSLFLKAPAAKTIHHAWR
                     GGLLEHILSISKILDFLASHYDSLNRDLLIFGAIFHDIGKIWELSYEGSLGYTDKGRL
                     LGHMLLACELVEKKASRILGFNEELKDMCKHIILSHHGKLEYGSSKRPKFMEAILVAM
                     VDDLDSKMNTIQTFIESERNVSDKWSRYHDLFDRYFMLDNLKERY"
     gene            20372..20887
                     /gene="epsC"
                     /locus_tag="VIG33_03475"
     CDS             20372..20887
                     /gene="epsC"
                     /locus_tag="VIG33_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEY8269922.1"
                     /translation="MLGKVKELLQTYKTYDPAAKSLLEIGLLYPGPKALFYHRCAHWF
                     YRRHFYFMARAIAEFSRATTGIEIHPGATVGRRLVIDHGMGVVIGETAVIGDNCIIFH
                     GVTLGGIKFDPVKRHPTVGNNVMIGTGAKVLGPITLGDGCRIGANAVVTKDVAAGSII
                     VGPASSVLEKR"
     gene            complement(20823..21575)
                     /locus_tag="VIG33_03480"
     CDS             complement(20823..21575)
                     /locus_tag="VIG33_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8269923.1"
                     /translation="MTSSMKLKDYKILFLHGKTQLLTDIYHPLKAHSASLTDLTIFEG
                     LDHQNFDLLIVDYSISDMTGILLYKKISELTNYGLVPAIFIADTKSYDHRLNAFEIGA
                     ADFINRPFESHDLVKKCLLHIKNRRRIAEDESIHIGNLILFPRSQTVTINGEPAPLTQ
                     LEYKILYCLVSNPRQVVSRSEIYAKVWGPGHSRSGRLDTQLYNLKKKISKFTGKIKSV
                     NKVGLRILIAESTFSQEQKKQAPQLSTPQPHP"
     gene            21787..22872
                     /locus_tag="VIG33_03485"
     CDS             21787..22872
                     /locus_tag="VIG33_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY8269924.1"
                     /translation="MKKQIMSAALGLMPLFAWAEFTPYASILVGIENGKSFNQGTVSD
                     NNGQLQSATLIEDYGSFFGVRGHENLDPNLRLIWQIEQYVTVDNSGFGADKNTLANKD
                     SYLGILGNFGSVRIGRLSTIPKNDMEYVDPWEYDGKSVNGLAMFARLDNRLNNTLKYE
                     SPGYFGAKLIMLYSPDEVKDVNGKAKDVFNAALLYKDATYFAAYSYYIKNNVHNGEDP
                     DVWNRIEAGVDQQIWMLSLGFQKVNGYSRSLAYNPSAYEAQKSVLLTGAESIQGTEYS
                     ATLNYTFQEKLTYRLSYAQGIDMEINGVGVDDTGYKHMVFGANYRLSSKSKAFIDYGQ
                     VNWNGNYFGTGTRITENNLALGLTLDI"
     gene            complement(22869..24449)
                     /locus_tag="VIG33_03490"
     CDS             complement(22869..24449)
                     /locus_tag="VIG33_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8269925.1"
                     /translation="MLWIDHFGIRIKILLTVFMTCFICGGISLIISIHFNNKELHKGL
                     MEKARTIHSRLDVAAKYVSSQGGLPPLIEKITQKYKDPSQILDEEKKIILQQVPIYAA
                     MKIGAEGSETENYTFRVFSNEPRRKENLATLREMEVFKKFESTPDLKEWVEDNDQKVT
                     VYRPVRLTESKGCFVCHGDPAKSPWGNGKDLLGYQMENWKEGKLHGVFAISSDLAAIK
                     AAQAQAGVISSSTYLGIFIGVGALLALLLAAFTIRGPIQELQKLSGFLNESGGQINST
                     SQKIADSSQSLSESSTQQAVSLEETVSTVKELTSMVRLNTDNSKQSAILASAARESAE
                     KGEREVHALIESIQNISTDSKRIEEITTIIDDIAFQTNLLALNAAVEAARAGEQGKGF
                     AVVADAVRSLAQKSAAAAKDISELLKKSSERIEKGNHQANQGGLVLSEIVGAVRKVAE
                     LNNEIASASEEQSHGISQIGKAMTQLDQVTQQNASVSEEAAATAEELSSQSTNLRSNA
                     ELLEKIVRGTRKQMPRDL"
     gene            complement(24579..27200)
                     /gene="hrpB"
                     /locus_tag="VIG33_03495"
     CDS             complement(24579..27200)
                     /gene="hrpB"
                     /locus_tag="VIG33_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HEY8269926.1"
                     /translation="MSSRPISPPLALPIDDFLAQALKSLETHPNLVITAAPGAGKTTR
                     LPAFLAKHGKGKTLVLEPRRMAAIAAAHRVAEEQAWTLGEDVGYQVRFANKTSANTKL
                     TFLTEALLSRKLAQDPELQGVEYVILDEFHERSLHVDLALGLLRELQELGSPLKLVVM
                     SATLDSPKISQYLSEAPILEVPGKLFPLEIKYQKNSQLLQTSPAFFENLVAHVKEAER
                     STSEDILVFLPGVGEIDRAFRQLETWAQEKQIDLVPLHGSLALEAQRKALQKGPHRKI
                     LLSTNIAESSVTIDGVSCVIDSGLAKNMKFDLRTGFSRLEMGRISKASAQQRAGRSAR
                     QFPGVCYRLWNKHDEGSMPGHDVPEIFRAELSESLLFLAAQGVSDFDSFTWFEKPAAL
                     SLKKSSENLQNLGALDQKNQITALGRQILQWPLPPRLAKLMLLAVEMKVPRLGADVAS
                     LVQERDFMVKQKTESSTENYLADRYESDLLLRLDILQQFRSGRAPRHTHPQGLQTVDQ
                     AAKQILSYVKNAPIQSHSHSRSLDLSLALQRLLLRSFTDRLCRRRSLGSDRARMIGGR
                     GVRISPESLVKESEFFLALDGVETSQGAETLVSLAAGFNKDFLFKELGHEIELKKDLS
                     FDRDKGQFYSREEKYFRDLPLEEGSVSIAQAKEVSEHLPQILTREWEWVLKENEALAQ
                     WLERLAYLMETREKLSKDSLREKLVRMVELAGEKNIFSEEQKYEAFGMASSGEKDFKT
                     VAHKDLVYFFESLLSSEVAQFLKSEVPAKLQVPSGNFLRVQYPANRDPFMEVRIQEIF
                     GWTETPKLLGGVQALTMQLLGPNYRPMQITSDLKSFWQNGYPEIRKELRTRYPKHSWP
                     EDPLSAPAVAKGRATKL"
     gene            complement(27197..27865)
                     /gene="rsmI"
                     /locus_tag="VIG33_03500"
     CDS             complement(27197..27865)
                     /gene="rsmI"
                     /locus_tag="VIG33_03500"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEY8269927.1"
                     /translation="MLYVVATPIGDVSEISLRALEILKSAEVIICESTKEASKLLRAH
                     GISGKTYELLNEHTTTEEVKALAEICTHKQAALVSDCGTPGFCDPGAHLVKLCRQKKI
                     QVKSVLGASALMGLLSLSGERIDQFIFRGFLPAENEARKKALQDIQKEKRPLILMDTP
                     YRLKKTLSDLQEFFPKRRLLLALNLTQTDETLLEGTADSLAKQLPFEKAEFMILLYGE
                     GSNY"
     gene            complement(27865..28617)
                     /locus_tag="VIG33_03505"
     CDS             complement(27865..28617)
                     /locus_tag="VIG33_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8269928.1"
                     /translation="MTQSSHLLHQILVKIEEFQNQGLRSLVVFDIDSTLFNVSPRVEK
                     ILMDFAADPVHKVNFPKQVQILKNIRTQGNDWGFKNALERAGLDGHHPEFQDALHKYW
                     YQRFFSNDYLHHDLPYEGAVDYVNAVVKRGAEVVYLTGRDIQRMGPGSVQTLKQVGFP
                     LDERQTRLVLKPHKSLDDAEFKTDWFAGLPQDQYAKMWFFENEPVNIHHLRQRLPAVS
                     VVFFESTHAGKALPPEDIPKIMDFLFTKQRKK"
     gene            28722..29648
                     /gene="phnD"
                     /locus_tag="VIG33_03510"
     CDS             28722..29648
                     /gene="phnD"
                     /locus_tag="VIG33_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8269929.1"
                     /translation="MKKGLRLLVVLTSAVILAACTGKKAELGTAENPIKIHFVPSVDA
                     KVIEDGSKLFKAFLEKNTPYKYEISMPQSFVAVVEAFGTKRADVAAINTFGYVLAHDK
                     YGAEARMTVIRHGFSTYQSQFIAKADGPIKSIADLAGKKVAFVDPSSTSGYLLPLKTL
                     KTKKIEPKETVFAMKHDSVVSMIYQGQVDGGATFYSPPSKDEKGNEQLEDARRLVLTQ
                     YPDVEKKIKILELSEPIPNDPIIFRKDMPEEIKAKVVDAIFAFVSTPEGKDAFNKIYG
                     VTEFKKSTDADYEPVRKMLNELGKNAADLMKK"
     gene            29674..30444
                     /gene="phnC"
                     /locus_tag="VIG33_03515"
     CDS             29674..30444
                     /gene="phnC"
                     /locus_tag="VIG33_03515"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEY8269930.1"
                     /translation="MSQPILSIKNLVKTYPNGVQALKGVSFDVKRGEFLVVIGLSGSG
                     KSTLLRCVNRLHDPTSGQIVFEGKDIAQVQGEEVRSLRKHIGMIFQHFNLIPRHSVLS
                     NVLMGRLGAMGSFQSIFGFFSDEDKAKALKYLKLVGIADKAKIRADQLSGGQQQRVAI
                     ARALAQGPSILLADEPVASLDPATCHTVMDYLKKVNTELGITVVCNLHFLSLVRQYAT
                     RVIALKGGEIVFEGKPSDITEEWFQKIYGQGSKEVHIN"
     gene            30456..31493
                     /gene="phnE"
                     /locus_tag="VIG33_03520"
     CDS             30456..31493
                     /gene="phnE"
                     /locus_tag="VIG33_03520"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEY8269931.1"
                     /translation="MKSYVFRRTFFDSFIFAYFAMILLVVAAQPTPEQMLNLGQNFLM
                     FVIAMVIGAALSAGLNKSKNSSAHTLGESLFEPAYKKAEKAERFWYKTFAGWQLVIFL
                     GVTFIVGLKSTKFSVTELLNEDGFAGALRLFKGLFNPNFEILPKAVLNIIETVFMAFM
                     ATTLAIPVAFVLSFFCAKNIMKSPKAYAVYMLLRTLLNFTRAIEALIWAIIFSVWVGI
                     GPFAGMLALLIHSIASLTKQYSEMVETVSEGPIEGIQSTGATKFQTVWFAIVPQVILP
                     YISFTVYRWDINVRMATIIGLVGGGGIGTMLIQYQGQAMWPEVGCIILVIAIVVWGMD
                     QASAVIRESLK"
     gene            31510..31917
                     /locus_tag="VIG33_03525"
     CDS             31510..31917
                     /locus_tag="VIG33_03525"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY8269932.1"
                     /translation="MANIGIYKLLIRETHLDTYGHVNNAVYLTMFEEARWEAITARGY
                     DFEKVHQSGQGPVILEANLKFMKELRLREEITITTELVDYPSKIGHLKQQMIKADGSI
                     ACEALFTFGLFDMKTRKMIEATPDWKKAVGLEA"
     gene            32014..>32730
                     /locus_tag="VIG33_03530"
     CDS             32014..>32730
                     /locus_tag="VIG33_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8269933.1"
                     /translation="MYRTINNQLLNWKQSKKRKPLLLRGARQVGKTWVVRQLGQTFDS
                     YVEVNLEKQPELDQLFKENFGKPKLLVKQLNLLLNVRIEPGKTLLFFDEIQENKNALI
                     SLRYFYEEMPNLHVIAAGSLIEFALKEISFPVGRIQFHYMFPLNFAEYLIALGRSDLL
                     AEIQQLPSVKISDGVHEKINEELKTYLFIGGMPEVVQSYLDQRDMRLCSQIQNDLINN
                     FRQDFNKYASRSRVPHLRLVF"
BASE COUNT         8868 a         6854 c         7790 g         9218 t
ORIGIN      
        1 tctctaactt ttttatgagt cgaagtgcgc gtctgcattt taataatttc agattcattc
       61 cctgcaatta tttttgtaat atctcttaaa acatcttttt ttattttaag atcagtctga
      121 gaattcgctc taaatgctct cagtatctcg cgtcgttctg cctcagaaaa ggtatgccat
      181 aacaaggcgt tcgttcgcgc atgtgactct ttcaaaattg gagcgtattt tcgttgatag
      241 aaatcacgaa gaatttcctt ttgctcctca gtatatccaa gattgggaag aagaacttgg
      301 aggacactgg acccataacc gagtttcaat accttcctag gaactttatc caagctgcag
      361 aaaactacat cgatttcctc aagatataaa taaccgcgcg gattacgatc ggttgtttct
      421 gacgcgaatg gccagcgttt tttagcatat cttgcaaagt attcagctac taaaaaagcc
      481 tcttcagtcg tctcatatgc ttcagcaaac caggtctttt tccttgaaaa aagctttgtt
      541 tttaaatcac tcatgagact gcctttcaag ctgttatatt tacatataaa acactaaaac
      601 tgtaaatacg caagttactt tgtttataaa taactttgcg ttataaataa aaaaaacatt
      661 acaacaattt gaatttggtg tgattaaatg taagttttga tcaacgtgag gcgtatcttt
      721 ggctatagaa ttaggaactt cagatattcg aaaaactttt atgggcctcg ttcgtccgca
      781 tttgaataac aaagagaaaa aatatgaggc caagaaattt accggcctag aacttggtac
      841 gctggatgga atgatatatg atggcagagg tggaatagaa gcatggcaaa aactgctcac
      901 atatgtattt aagatctctg ataatgagat agatcaggtg cttttagaat tcaaggacta
      961 cctcaaagca agaatcaaaa tgacccccgg tgagttgatg tgggcacaat taggcgaaaa
     1021 acttactgaa gatgagaaga tctttttttg cgaactggca aaagcccaca agatgctaaa
     1081 acctcctttt gaaattaaac tgactaaaaa aacacgaaaa tccttgcgtt aaaggggaag
     1141 gaactcaagc agtcaatgaa tttaagctaa cagaaatgct acgaatctcc ttcaaaaaac
     1201 ggagagctta gttcttgggg tagacgataa ctttttactt aattttattt gcctatcctt
     1261 attagaagac aaagttaaaa aattaattaa aatcaagttt tcataaatct taaaaaagtt
     1321 tttttaaaat tcacagtttt aaacaaaatt tgagagtcgc ttgatttaaa acttattttt
     1381 ctttacattt ctctgtcaat agtccctgat tccgagtgta tttggaagtt agataaacac
     1441 ttatcgagca aagacttcca tttttttaaa acaaataatt taaaatttac acttcatagt
     1501 cctcaaacga gatatgatgg ttttccctac gaagatcgga cttcatgagc aattgaatga
     1561 aaacaacctg cattcataag caagacggga aggtttcaac taatgtctga tttaaaatct
     1621 gattccatac gtacttcaaa gaccgctcaa aaaaataagc ttcaaaatac tagggagcgt
     1681 actccagtca ttgaaaattt cgaggcgcgg ccagtgatcc tggccgtagt ggcttctcaa
     1741 tcaatagcgg attctatttg cgctgagctt ggttcggaat accggattga aacggcggct
     1801 aatggccgtg aaggtttgaa gaagactctg caaattcatc cggatctaat cctaacggat
     1861 ttgatgatgc caatagtaaa tggcgagcaa ttctttcatg ccattcgttc ccacgcggaa
     1921 attaagtcca ttccattttt gattttagct gccaaaggag aagaggagct tcgactcaag
     1981 cttctgcggg aaggcgcaga cgagtatttg ataaagcctt ttatgccaga ggaacttcgg
     2041 gttcgcgtaa aaaatttggt gcggcacgtt caggatcaga agtcgttggc tcacgaactt
     2101 ttagtacgag aacaagtcga agaaattttc agacggcagg aattccaatt aaaggaggcg
     2161 caaagaatca ccaactttgg gagctgggat tgggatattg taaaaaacaa acttacctgg
     2221 tcagatgaat cataccgtat ctttggatat gaaccccagc aaattaaccc tacctacgaa
     2281 gctttactct ctcgcgttca tcctgatgat ctggatcggg tcaatgaagc ggttcataaa
     2341 gtgctcttcg aaaagaagcc ctattccctg caacatcggc tcgtttggcc tgatggttct
     2401 gaaagaatca ttgtctcggt tgccgaagtt caattttctt cagagggccg tcctcttggg
     2461 atgattggca caatctatga cattagtgaa caggaagagg tgaaacgcag attggaagag
     2521 gccgaattaa aatacagcgg actctttgag tcggctgcgg atgcaattgt tgtggtggac
     2581 aatcatggca ggattgaatc cgtgaaccaa cagaccgtca aatggtttgg atattcccgg
     2641 caggaactcc tgggccaacc tgtggaaatt ttgattcctg aacgctttaa acaaactcat
     2701 atcctagagc gcaatcggta cctcacccaa ccctctcatc aaatcatggg ctccggacga
     2761 gagatcgtcg ccaaaagaaa agatggggga gaagtgccca tcgaagtttc gctcaccccg
     2821 ctgggatctg gaaaaaacaa agtcgtagca gctattattc gtgatattac agatcgaaaa
     2881 aaaattgaaa tgcagcagaa ttttttttca gaatctggcc gtattatatt ggaaacgatg
     2941 gacccccagg tgcgtttaga ccgcggggcc aatatgatga ttccgaaaat tgccgatatc
     3001 tgcctggttt ccacctatga acaaaaggat tttcacttca gtgccttggt tcattacgac
     3061 cctgcaaagc agaagctcct tgaagagcta actgccaagg attgttcctg gagaaacttc
     3121 cctttcaacg cgaaatcggt atccgaactg caagagccta ttcttataga agacgttgcg
     3181 gagggggctc tggaaaactc cgtcgcaatt aggaatatga atcagttgat gaagaggatg
     3241 aacgtaaagt cctatatcat actgcccatg gttgttcggg aacgacggat tggatctatg
     3301 actttagcca tgtctgattc gggaagaaaa tttaggcacg atgactttgc ttttttgaaa
     3361 gaggtggcgc ttcgctttgc ggttgccgcc gacaatgcaa agctttattg ggattcgcag
     3421 caagctatca aagataggga aaatgtttta tttattgttt ctcacgatct caaaaatcct
     3481 ctttccagca ttaaattggc tggcgaattt ttggaagaat atcatctccc tactgaacag
     3541 gatttttttc ttgtggccaa aaagattttg caatccgctg aaattatcga aaggcttatt
     3601 atggattggc ttgattttgg aaaagtccaa tctggaaccc tggtgattag aaaaaaatcc
     3661 gagagcttgg cggatattat tgagtcctct ctagaggcca tgaaggtcaa ggctttagag
     3721 agaaatatta agctcgtagt ggatttgccg aaaacggatg tgatgatctc ttgcgaaaaa
     3781 aatcgaattc ttcaggtcct ttggaatctt ctcggaaatg ccattaaatt cacttcggaa
     3841 aacggagaag tccgaatcaa agcgatgaag tccgacaact ctgtgcaaat ttctatcatt
     3901 gatacggggc agggcatcag tcctgaacaa attcctcgaa ttttcgatcg gttctggcag
     3961 gccacagaga cggcaaagct tggaaccggt ttaggtctgt cgatcgccaa ggggatcata
     4021 gaggctcatg gcggaaagat ttgggtcgag agcaaagtcg gtaaaggaag ccaatttcat
     4081 tttaccatcg caaccgctga tgaagtgaaa agtccgacga ctcctaatca cgcgcatgac
     4141 cataaatttc attgtgaata cttagaaggc aaacgtctaa aggggattcg tgtattactc
     4201 gtggatgatt ctcctgaggt cctctttatg atgagattta ttctggaaaa ggcgggggct
     4261 gaagtgagtc aggccaggtc tatgagggag gccctcacct cgctaaaaca ggagaagccc
     4321 catgtgatgt taagtgatat tgaaatgccg gatggagacg gctttgagct gctacgaaga
     4381 gtgcagcagt tatctgttga agaggggggg cgaattcccg ctgcggcact gactgggcat
     4441 agagaagaag acaaacttaa acaaatgaaa gaggccggct ttcaattgca actgcagaaa
     4501 cccgtgacct gtgaagaact tgtcaaagca gtgagtgaat tagcgggaaa actgagccta
     4561 tagccctgtt tataaagatg atcgatcaag aaatcactgc aagcgataac ggttttcaaa
     4621 tccaggtcgt acttcatttt cagatccctg tttagccgct tttgaaagac cttttttgat
     4681 attaaaactc agttttttgt catatttaaa atctatgatt gtagcgatgg attttgtttt
     4741 tttatctcgt aaaatcaaat tgactagcgc cgtaatcatc tcaattccaa aatgtttggc
     4801 gccgataaat ttttccttat tggacaccac atctatgtcc tttagcaagg gtttgaaata
     4861 cccccggact tgtccctctt tagaaagaac ttccgaataa agatctaagg ttcctttcgt
     4921 gaaggttaaa ggaacatggc tttttaaata aggatcaaga gtcacgatat caaaatcttc
     4981 cacttcaata tcgacactcc aaggaatggg ggttgcaagt ctattgaact tgccgccaaa
     5041 cttgaattta gaatttgggt ctatcaattt ggccgtcacg ttaaaggaag aaagggggat
     5101 ctggggactc ggagtaagat tcaggattct accattaatg gcggaaattt taagaggttt
     5161 attttccttg agggacttat atttttcaaa agtaatggag gagtcctgga gatcgacctg
     5221 ttctattttt agtggaaaaa aagtatctgt aatatcctgg gtttcttttt tgggaaagga
     5281 aagtttcttg aggtctttag ggattaacag atgtgcattt tcaattttaa tatccattaa
     5341 aatttttctt tttagaaggt gtcgccaggc gatagaaaga tcgaccgttt taacagttaa
     5401 aaaggtttgg cctgactctt taaatttccc cccaatgtct ctaaaacgac aagccccttt
     5461 taaaaaatct aaactcaaat catccatata caacgaatag gtcggcgaaa attcactaag
     5521 aaaaatatta gcctttttta aaagaaaaga agggagagca gctctcaata ttatcagtag
     5581 caaaattata aggaatggaa aaataaattt tttttcccgg taaaaatgag tctttctctc
     5641 ttctgatgcc atggagaccc tctcttttct cgaggttttc cattttaaag caaaatcctc
     5701 gtaaagggtg acttgtccaa aaagaggctg ggaaaattcc ttaatttttc aaatttgtca
     5761 gtaggcaatt catgccatgg gcgacaggcg cggaactccg cgccctgaat caattttatt
     5821 ctgaaattta ttattttccg aaatttaaag aggctgagcc aatggctcca gtgaaagtca
     5881 aacgcgctgg tttattatcc acggttcgag cgctgaccgg aaagctgatg atatagggtg
     5941 aggcaaaaga ggtgtgatag tgataaaggc tttggatttc ccgtggcaag agcttgatct
     6001 tggtggcttt gccttcgaaa cgttgtccat tcacatccaa gaaaaccttc cacagggttt
     6061 tgctcttgtg aagatcatcc cattttcttt ctggagaaaa gaaactgacg aaaacttcgg
     6121 tttgctggtt caggttgttt tcaagtttct tcttttctgt atcaaacgta ttatgatccc
     6181 attgaaaaat tctcgcattt tgatcgagct gcgcggtcgc catctctgga tttaataaag
     6241 tggccttgat gctcacggtg ttattcaggc cattgtattc ttcaatataa tccgtccatt
     6301 tatcaaccgt cgcttgataa tcggattcac tcataatatt aagaccattc ttattgacag
     6361 tggaggaggc gcacgaactc aatagaaaga ctaaaaatac ggtaaaaaga ggagatccaa
     6421 gatatttcat tttttttcta ctcccgccag aaagtgtctt aagtcttcaa aggcctgttc
     6481 acggatgatg tcgttataaa tttcgtgctt agcatcttta tatattttaa tttctttttc
     6541 cagactgccg aaatgttcaa aaaagcgcat ggcttccgcg gagctgatca ccggatcttt
     6601 gtcggagatt tgaaaaagag ttttcacttt caattgagga attccttcgt aaacaaattt
     6661 aaaactttct aagaagccta aatagaccgc cgcgcagacg cgggcgtgtc gataagggtc
     6721 atgctccaat tcgcggatga cttcagggtc acgagtgacg tcttgattgc caatttcatt
     6781 ccacatggtc aaggtgggca gcagtttcgc gatccattca gcgcccagtt tcttgtaggc
     6841 ggggatttcc actccaatgc caaagagtgg ggaactcaat accacggcat ctggtttgaa
     6901 ttccgggtgc tgcagcagag ctttgatctg cactaagccc cccatcgagt gcgcgagcaa
     6961 caccacggga ccttttgtga tgagtggctg ggcgaaaacc attttcaaga attcaacata
     7021 gtcactgcaa taatcatcaa aactggaagc atatcctcgg gctccttcgg attttccatg
     7081 accacgcata tcccatgcat agatattcca gacatcgtct ttcaaagctt ccacaacgcg
     7141 tttataagaa tccgaatgct cgccatggcc gtgggtgatg acaattgtgc cacgagcacg
     7201 ggggttttcc caaagctgaa aaaaaagatt cgcattgtga gcgcctataa agtggccttc
     7261 taaatttttg tatttatttt gcgtcataga tacctgcctc gctctatatt ggcgaactta
     7321 cctaagacca cgcaacaaaa ttgtatcttt tgcttgcgga tctgttcctc ttttgtcttc
     7381 ctcagttttg gtcaacgcta ttactttggg gacaaaagga tagtccaagt tataatgaat
     7441 gccacgggat tctttacgtc gaagggcgca ctccacggaa agctccgcga cgatggcgat
     7501 atttcgcaat tcaaggatgt cgctgtgaat tctaaagtga gaatagtact cacgcacttc
     7561 gctcaatata tttctcagtc gatgctgagc gcgctgcaga cgtttgttag agcggacgat
     7621 gccgacataa ttccacatca agtgtcggat ttcctcccac atatgggaga taacgatcat
     7681 ttcatcatcg ttggactctt cgggataatg ccagtctttt ggattatgag gacaaatctc
     7741 aagagcggcc cactgttgtt tcaaactttc acttgcattg tgcgccatgc acagacattc
     7801 caagagcgaa tttgaagcca aacgattggc tccatgcaag ccagtgcagg cagtttcccc
     7861 aatggcacgc aggccaggga tatccgtttg tccctgaata tccgtcagaa ctccaccgca
     7921 cagataatga gcggccggca ccacggggat cggcgttttg gtcaaatcga tgccatactc
     7981 aagacatttg ttgtaaatca ccggaaagcg tttgcgtaaa aattctgcat ccaacttggt
     8041 catatcgaga taaacacaat gcgcaccgga ctttttcatt tccgcatcga tggatctggc
     8101 gacgatatcg cggggggcga gggagcccag ggaatgatat ttacgcatga aggcttcgcc
     8161 ctgggagttg atcagttcgc cgccttcgcc gcgcaaagct tcggagatca aaaaatttct
     8221 cgcggcggga tgatagaggc aagtgggatg aaattgcatg aactccaaat tcgcaatgcg
     8281 ggctccggct cgataagcca tggcgattcc gtcgccagtg gcgccactcc aatttgaagt
     8341 gtaaagataa actttgcccg cgccaccggt ggccagaaca gtggattttc ctaaaaaggc
     8401 cgcgacctca ccggtttttt tattcaaaga gtaggctccc acacatcttg tggggctcat
     8461 gtcgtaggta tcgacttgtt tattaatgat caggtcgatg gcaaaatgat tttccaaaag
     8521 ttcaatgttc ggattttcgc ggatgcgccg gagcagagtt tgatgcactt catggccggt
     8581 ctggtcttca aaatgaagaa tgcgtctata agaatgcccg ccttcgcgcg tcaaatcaat
     8641 ggagttttct tcattgtcca ctcctcgacg aaggtcgaag tggacgcccc agtcgagcaa
     8701 gtcttggata cgatcaggag cttgctcaat aaaattcgcc acgacttcag gcttacagag
     8761 gccggccccc gccgtcagcg tatcttgaat atgactttcg aagctgtctt cttgggacat
     8821 caccgcagct atgcctccct gagccatatc ggtattgcta tcgagagctc tttctttcga
     8881 gagaatcaca acttttccag aggaagccat ttttaaagcc agcgaaagtc cagctaaacc
     8941 ggatccaata atcaaaacat cacaacgtcg catttccacg ggttctccct ctcacagtgg
     9001 cctctagctt atcgacgagc cgtaggtctc gtcgatgtaa agggttcttt ttaatgctct
     9061 ttattgtctt tggcaaagga ctcttgcttg attcttagcg gtttctaaat ctacaataga
     9121 atcaatagtc tagaacatgt gtgtttgatg tttaaggaag caaagcaaat atgaagttga
     9181 aactcctgat caccatcctg gttttggcca ctcttttttc aggagtggtt ttatggcgcg
     9241 tggatcggtt tgtgtacgga gatcgtatgg cctgggcgga ggctcaggcg cgcacccaac
     9301 tttcttcgct gtctcaggcc atcaatacgg aaattcaatc ctctcgccgc atgctcagct
     9361 ccgtcagcaa tgacaccttt aaacgggagt ccaccaactg gaaagctttc cagccctatt
     9421 atgccatagc tttgatgact tcgcagagtg gagcgctcag catttcgcgc ttggtttcta
     9481 aagcagattc accggcagcc aattggacgg tcaatgattt gactcagtct attggcttta
     9541 tgggacgggg acttgagaac cgcggcacca ttttgcttcg ctcctttaaa gatgcgcaaa
     9601 aaaatcgcca tgtggcggtg atttttgcgg gcggcggaaa tgcttatctt ctgattggca
     9661 ggggggagaa ctttcagtcc ctcattgaat cacaaaaagg ttccatgagc gcgatttcta
     9721 ttattgccaa tgatggtttg accatcagcc atgcggttcc tgattacatc ggcaatgtaa
     9781 tgagtgagag cactctcctt aaagaaatcc gcaccacggg tgcggctcaa gggctgggtg
     9841 tttatctgca gggcaagcgt cctgttttcg gtatgtattc gcaaattcct ggaacgaatt
     9901 cttatgtgat ctccagtgtc cccctcgaag acctcttgaa agggcgtctg tctttggcat
     9961 ggcagtttgg ttttatggct gtgggtattt tgcttttggg agccgccttg tatttctggc
    10021 aagaaaaacg ggtggtcagc gcagcacttc cgaaaaggcc gcagtccgtg aagacgactc
    10081 cccggccttt ggaatacgag gcgccggtgg ctctaaaaca gccgatagtg gcgacgcctc
    10141 aggaactgca gcaggaaaaa accgatgcct atcgtcaggt ggccacggct cttgggcaag
    10201 aaatgcgctc accgttggct tcgattttag gtttttcaca aatggttttg tcgaaaactc
    10261 aggaccctga tgtggtccaa gccgtggaat ctattttgcg cgaagcccgt tcttctcggg
    10321 atgttgtaga aaagctcgtt acttttgccg gagagcggag cacggataag acaaattcta
    10381 aaattgaagg tcccatcata caggccctta aaaacttaga cctgaagctg agccaaaaag
    10441 gcatccgcgt cgagaaggat tttaaggaaa ctccgccatg gcccctggct tttgccgatg
    10501 tcactaaagc ttttgaaaat ctttttataa atgccattga agccatggag cgaatgcaag
    10561 agaaaacatt gagaatctcc agttgggaat ccgccgaagg gcttcatgtc aagattgcgg
    10621 attctggtga aggcattgac gtggaaaatg tagcaaaagt ttttgatcct ttcttcacca
    10681 ccagatcttt tgcccaccat gtgggtttgg ggcttccggt ggtggtggga attctgaaag
    10741 agcataacgg aaaaattcaa attcattctc agccaggaaa ggggactcag gtggaaatta
    10801 tctttaaagc caattctgta gcgggcacgg ggacgtcttt accaaaagtt ccgccccctc
    10861 ctcccgctaa attcgagatg ccgaccgact tgccaaaagc acctgagatc tctctcgaag
    10921 cggaaattcc gaaagtcgaa gtttttaaaa aacaaatttc gcaagaaaaa ctcaccgaca
    10981 tcaatgtgga taatttgttg gagttgaccg aagatgaaga acctttgcgg tttttagaag
    11041 gccatggttt tgagaattct gtcccttcgg cgcccttgcc acaaacgcca gaacccaaga
    11101 cggtggatcc agcagtggtt ctagatatgc gcgtagcggt gcccctgaaa aaaacggaat
    11161 tggattctta caaggtcgaa attcgaagac ccggaaagag gacttgattg aatatccgag
    11221 atcatagttc tcagtttagt atttttacat atagtaccga tgtggatcaa ggtgccttgg
    11281 taaaggtatc cttagcgcaa gcgggctacg atgcctatta tttccaagag ttggatattt
    11341 ttttatcacg tctcaaagag atgacccctc atgtggtggt tttttcgaca atgacggtgc
    11401 aaggttccct cagtgaattt gtaaatcagg ttttagaagt cagttcagaa attaaactga
    11461 tcgccatggc ggaattgccg cagtttgaat ccctttctcg ctatagtacg acgggcttgg
    11521 ccgatatcgt gtctttagaa ggtcccagtt tggatctgcg cgtcctgtgg tccgtcgatc
    11581 aggcttgtga ggctctgtat ctgacttatc aaaatgagca gctttatgcg gattggaaag
    11641 aagcccaggg tcttaaagaa aaattgcaaa atcagatgga tgatcagaaa gcgatgcaag
    11701 cggaggttac gcagatgctg ctttcaaacc gggttgcgga ttatcgctct tcgcagaaca
    11761 aagaagaggt tttgcagaaa tattttgaac gtctcgaaaa ggttcccgct atttatttta
    11821 aatatttaag ttcggttcgt tctttcgtgg ccacccatgt atcgggattt tcgccccagc
    11881 aaattcaagg agtgggttgc caacttgaag accccagtct ggataattta tcctctcagg
    11941 tcagcgtggg tttattgcct cctgtcatga tggagatggt gcagaaggtt ttcgctattg
    12001 aaaaccccag ggttttgccc ctgtttgtcc aaggcgaact tgaaggctta gtggtgtact
    12061 ccggggacct gggtaaagcc gatagtttgc gagtggggga ggagtttagc ctctttgccc
    12121 tttgttattc ttatttctgc ctggagaaaa aagtcgaggc cctcgatgtc atggatccgg
    12181 tgaccgagct ttataacgaa aaattttatg ttgataagtt gcacgaagag ttggaacgat
    12241 cccgtcgttt gcgccaaccc gtttcggttt tgaaaatctc tattgatagt ttctctgaaa
    12301 ttgagcaggt tctgggaagc aacgcttgca atggggtttt aaaaaacctt ggcgactttg
    12361 tgaaacgcac tcttcgtatc aatgatttcg cctgcaggac aaaggccaat gagatcggcg
    12421 tgatcttgcc tcattgccat agaaagggcg cggctctgtt gggggagcgt ctgcgacgca
    12481 ttgtcgagag cagctcgatg gtcgatagtg gggtgaagat ttccatcagt atcggggtca
    12541 gtgaatatcc atccttggcg gattccgtgg acactctcga tggaacagcc tcaaaagctt
    12601 tagtgcatat tatggaaaaa ggcggaaatc gtatttgtct ttataagccg ccagagagct
    12661 ttactcccga atttgaagtt gtcggttagg agtattggtt aggagtgttg cgtggtggaa
    12721 atgtatcggc ggactctggc ggaaatcaat ctggatcacg tcgcccacaa tttcgaagtg
    12781 ttgaaaaaag cattttcaca cgcgccgttt atttgtccaa tggttaaagc caatgcctat
    12841 ggtcacgggg atgttgcggt ggcactgtgt ttggaaaagg tcggggctca gcatctcggg
    12901 gtgtgcctga tagaggaagg tcttttgctt cgccgaggcg gagtgaaagc ggagattctg
    12961 gtgttccgcg gttttgatcg tgaaggagca aagacaatgc ttcaacacaa gttaactcca
    13021 gtggtcgcga cttgggatca acttcacgcc ctgcaaagtg tcgccagtca ggcaaagcaa
    13081 cccgtgcaaa ttcatttgaa atttgatacg gggatgaatc gcttgggttt tcccgtggca
    13141 gaggcccagc gtttatttga attgtgctgg cagaattcaa aagtgcgggt gcaaggcatt
    13201 ttgactcatt tatgccatgg cgaggatgct caggacccag cgggcgatag tgcggcacag
    13261 ctttcgagcc tcgcgcaggt tatgaaaatt tttaagcctt taaaccccgt ggtgcatagt
    13321 ttgaattctg cggggatttt gaacagcctt attatggcac gccgtcaaaa agatccgcat
    13381 catcctctca gccgagttca gtgggggctg cggccggggt tgatgattta cggctatagc
    13441 ccgctggcgc agaaggatat tcttcctttg aagccggtca tgactttgaa gtccgtggcg
    13501 tccacgtatc gcgtgcttaa agccggtgaa aaggtatctt atgggggtac ctggacggct
    13561 tcaaaggcgt cggtgatcgc cgtggtgcct attggttatg ctgatggcta tcaccgcatt
    13621 ttatctaata aggggcaggc tctattttta ggacagcgtg ttccggtggt tggacgcatt
    13681 tgcatggatt ttttgatggt cgatgtgacc gaagtagtgc tacataagga ccttaaagag
    13741 cttgaagaag agtccataat tttattcggc caagaccgga atgggaattg tttatctccg
    13801 gaggaactgg ccctacaggc tcaaaccatc acctgggaaa ttttgaccag tgtgggagag
    13861 cgggtcccgc gagtatatac cggagacatg gcgaaaaaat tggtgaaaca gttggcgaaa
    13921 cagcggggag atgcagattg agtcttgtcc agaagatgtc ccagataatc gatctgatcc
    13981 ttaactattt ccgaggtaaa atcggtggat ttgtcgaaga agtgggcaag attgttttat
    14041 tcatgacttc cagcatccgt cttctttttg ccaaacctta tcgttacact gaaatgattc
    14101 gtcatatgga gtttgtgggg aatcagtcca ttggcattat ttgcctcacc ggcttgttca
    14161 caggtttggc cctttcgctg caaatttatt tgggctttaa aatgtttaat gccgtaaata
    14221 tggtgggccc tgtggtggcc ctcggtatca cccgagaact gggaccggtg ttgacgggtt
    14281 tgattgtcgc cgcccgtgcc ggtggagcca tggcggcgcg cttgggaacc atgcgtgtaa
    14341 atgagcaaat tgatgctctc gatgtcatgg gagtgaataa caaacagtat ttgattgctc
    14401 ccagaattgc ggccgcggtg atctgcatgc ctttactgac cgccgttttt gactttgtct
    14461 ccatgctcgg aagttacttg ctttgcgtga aacttgtgca aattgatgaa gccgtcttct
    14521 ggcaaaagat tcgtgatatg attcaagtcc gccatattaa tgaaggcctt ttcaaggcgg
    14581 cgattttcgg attgatcttc gcggtgatct gcacctatcg tggcttttac acaactgggg
    14641 gcgctaaagg ggtcggcgaa gccacaaatc aaggagtggt tcaaagtatg gtgatgatca
    14701 ttattttaga ttactttgcg ggcaacttga ttcgtttgtt ctacctagtg atggggatga
    14761 attaatggaa gaccactcta aaatcccctc gttcacggct tcggcggtct ctctgcgaga
    14821 tgtaaagaaa accttcgatg gcaatgagta tattttaaaa ggcatgagct tggaggtccc
    14881 tcagggaagt ctgaccgcca ttattggctt ctccggaacc ggaaagagcg tggtgttgaa
    14941 acatattctt ggtctgtatc agccgagtgc tggtgaaatc catgtcctcg gagtcaatat
    15001 cggtcttttg cctcccaatg agctcacgag atttcgccaa aggtttggag tgctttttca
    15061 atccgccgcg ctctttgatg atatgacagt cttagaaaat gtgtgtttcc ccctcgttga
    15121 acatcgccga gatctgcgaa gcgcgcaagt gattcacctc gcggaacagc gtcttgacca
    15181 ggtcggacta gacaaaaagc actatcataa actaccaaat caaatcagcg gtggtatgca
    15241 aaaacggaca ggtctggcga gggctctcgc cctagatcca gaaatattaa tatacgatga
    15301 gcctaccact ggattagatc ccattcttac agaaatggtg gataatctaa tattggacac
    15361 gcataagtcc cggaaaggac tgacctctat catggtctcc catgatttag ctgccgcctt
    15421 tcgcatcgcc gattatgtcg taatgttaga tgccggaaaa gttctgttga gtggtacggc
    15481 taaggatttt tttaatagta atattgaatt agtgcagaga tttgtcgcta aaggaatgaa
    15541 gcattcatga actgggtaaa atcaccagaa tttaaagtgg gactgctggt tgtcgttgtg
    15601 ggctcgctca ttgctttcat gtccatgcaa gttagcgaag atccaagtta tttgggtcgt
    15661 tcaaaaaaag cctggttcct tctcaaaaac gcggatggct tggttaaaga ttcagcaatt
    15721 agaacagcgg gtatccccat tgggattatt cgggacatta ttttgcaaga cggccaggcc
    15781 cgtgttgata tcactgtgaa atcagaggtt catttgacgg tttccgcggt tgtggaaatc
    15841 cgggcgcagg ggattctagg cgacaaacac gtcgaaatca atccgggagt tccttcggat
    15901 ccgcctcttc ctgagggggc tcagatcctg aatgtgaaag aaaaaggctc tttagataat
    15961 ttggtgggtg aggtctccga gatttcttcc tctttaaaaa ccgtttctga aactttgaaa
    16021 gaagcgatca ccgaagatgg aacccgtaaa catatcttgg ggcgcatcgt ttcgaatatt
    16081 gaaaaactga cgggcgatat cgcgcaagtg acgggagaaa acaaagagaa aatcggtgaa
    16141 attgtcgatc aagttcatga tgtgacctcg actttgagca atctggtaaa tgatgaaggt
    16201 gaagacggct ttaagaaatc ttggaaaaaa gccgtggctt ctttgagtcg acttgattcc
    16261 attatgaaga acgtcgatga aatctctggc aagatcaacc gaggtgaagg aaccatcggc
    16321 aagttagtga atgatgaaag caccgttgaa ggtctgaata ccacgattga aggtatcagc
    16381 ggaatgatgg aaaccgccaa tcgtattcaa accggatttg atttccatgc cgaatatctg
    16441 ggagaggtca aagccacaaa gtccacggtg ggaatttata tccagccagg tttggatcgt
    16501 tattattacc tcggcatcgt ggatgatcca gcaggggtga ttgaacgtga aggcagtgtt
    16561 tccacgacga acggggtggt caccaccaca gaccaaacta aaaccttcta caataaaact
    16621 aaatttagtc tcttgttcgc caaaaacttt tataacttaa ccctccgcgg gggtctcatc
    16681 gaaaatcaag gtggcatcgg gattgattat cttttcttcc ggcgcaaact taagctgtca
    16741 ctggaagcct ttgactttag taagctgaat cttcgagccc aggctcagta cagtctttct
    16801 catgggattt atgttttggg tgggatgacg gatatgatgg ataagtcaga tcgtcggtcc
    16861 ggttatcttg gggcaggtat ttatattacg aatgatgatt tgaaactact gctgtcgtcg
    16921 aagtctccat tttaaatgag ggtatgatga aggtgctaag tggccgccgg tcctgaaaac
    16981 aatcacttaa ccaggaattg gttggtgaag tcttcaacaa agattctagg ccccttcact
    17041 cgtgaagagg tgatgtctct attgactcgc cgtcaaatca caatcatcga tgaggtccgt
    17101 caacccgagg ggcgttggac ctacatccga gaaaaccgtc actttaaaga agtggttaag
    17161 tctttacgct atgaacagga ccactctaaa gaagatacca tgacgagcac ggcgaccctt
    17221 ggggccacca tgaccaagac agatccagcg accgtcagtg atgagtttac accgacgccg
    17281 atcacgccga tgaaatctcc gcccctgcat attcaaaagc ccggtttgaa ggatgtgact
    17341 ccgctcaagg agaccagtct tcgtcctcag gggggggcag gcgcagctaa aagttttggc
    17401 acgctttccg atcaaagagt gcaaagtaaa attcagaaac agaataggtt gcttcgtggc
    17461 attctgtttg tgatcaccgg cgtggctgtc ttatttgcca cccttcactt tatttttaaa
    17521 gaaaagaaat ccgatatcaa ttatgatcaa cttttaagat cagcactgag ctataaagaa
    17581 attggtcttt atcaactctc tttacaaaaa tataaaaaag ccttttctat caaagagccg
    17641 gatttggaaa gccaattcca gatggtgttt ttgttgatta atgaagatcg acaaagtttg
    17701 aacggccgtc gtgtgataga acgcgcgctg cagacggaag gccgtaatcg tgttgaaatt
    17761 atcaatgctt atttggggat ggctctttcc tatatgatgg agggggatct tcgtcaagcg
    17821 gaagagtatc tgcaaaaaac tttgggttat gatgcgaata atgaaaatgc caaaatcaat
    17881 caagcggtga ttcttcttaa aaaaggaaac tatgctcaag cgttgaagag ttttgaagct
    17941 ctgagcaagg tggattctcc agtttaccct ctggttctgt tgtgtgaatc tttgtccttg
    18001 attgaggttt caagaggtca ggcgaataag gatcgactgc ttaaagcggt gagcgaaatt
    18061 aaatcttata gcgctaaatc acattttttg cgaaaagagt tgtccttatt gatgctttat
    18121 ctgagccggt tgggcggaga tcctgaaggt gaacaggatg ccatcaatac ttttctggaa
    18181 gaaccgcaaa acttaagccg ccaatacgcc cgagaactcc aagttgattg gcgtaatagc
    18241 gagtgggact ttcttgaacg atattgtgcg gaactttata actcgacaaa aggcccggcc
    18301 aatttgaagg ccgttcgcgc cgtctgtctt atcgagagca accgggatat ggaagccgtt
    18361 aagtatttgg atgaatccat ggcccaagct cccaaagaac tcaccaccct tcaagctcag
    18421 gcgcagtatt tgtggaaatt gggacgttta aaagaggctc agctattatt tcaaaatacc
    18481 gattttaaac aagaccgctt ggctctgtat attcagggag aagcttgttt acaagcgaaa
    18541 gatttaggat gtgcggaagc agcgtataag attctctcgg aaagagatta cggggatgtg
    18601 ttagctcact atggtctagc cctgttgacc caagcgcgta atgataaggt tcgcctacag
    18661 aatgaaatta aggcgggttt tgaagctgag agtaactttg cgcctttgat tgaaattcga
    18721 gaccaaatgg aatccatgtg aaaaaagggt ctcttttgtt caccttgagt attttctctg
    18781 gactgatggc ttgtacgaaa ccaggcagtt ttcgcagtga agaccctcag gctgtgaaaa
    18841 tggtgaaggt ggacgagatt cctgaagatt ttaaaatccc ctcaaatatt tgggaactaa
    18901 tcgcgaaaaa cggtgacgag aaatcggtcg aggtcaataa gggaggggtt ttttattcca
    18961 ctgtaaagct ttttctgaca gaaaagaact ctcatatttt gcagaatcct tcctttgtga
    19021 ttgagcttcc gcgaggaggc ggtattgtcg atatgtcgcg atatttaagt gggcaacagg
    19081 gaagctttta tgtggggttt gagctgcctg ctgaatttca agaggggaag aactttaaag
    19141 cgatttatat tagtaaagcc agaaaaagta agttggatga tcgggtgttt ggtgctggct
    19201 gcaataaata ttttgacatc actgtgaagt ttttagaaat gatgaagacg gaagggatta
    19261 aagccaatac aactcgtcaa cgtcatgtaa cattattagc ggggcattat attttctctg
    19321 tgataaagga caatcgcatc catgtaactc aggtgaccat cacagatagt cagaataaaa
    19381 acttactttg cgaggcgcta tgatggaaaa gaaaactgtg cgcgaattga aagacaaaga
    19441 cgctgtggac agcctttttc tagtaaaaga aaaaaacgtc aacgtcggaa aaaacggccg
    19501 cccctttatg agtcttgtgt ttggtgatca gaccggctct atcgatggtc gggtgtggga
    19561 ccgggtggaa gaattaagcc aggaatttga agtcagcgat atcctccggg ttaaaggtca
    19621 aattcaaatt tttcaaaatc gcaagcaact cattgttcat cggctagaga aaccggatca
    19681 gagtgcttta aatgttgaag atttcattcc cacttccgga aagaatacgg atgacatgtt
    19741 tatggaactc ttggtgttgg tacgctccat gaagaatgat catcttcgct tattgaccct
    19801 ggatgtgatt gaggacgcgg agatcaagtc tttgtttctg aaagctcccg ccgctaaaac
    19861 aattcaccat gcctggcgcg gtggcctgct tgaacacatt ttatcgattt caaaaatttt
    19921 ggattttctg gcctctcatt atgattcttt gaatagagat ttattaatct tcggagccat
    19981 ttttcatgat attgggaaaa tttgggagct gtcttatgaa ggatccttgg gttataccga
    20041 taagggtcgt ctgctggggc atatgctttt ggcctgtgag ttagtcgaaa aaaaggcttc
    20101 gcgaattctc ggatttaatg aagaactcaa agatatgtgt aagcacataa ttcttagtca
    20161 tcatggcaag cttgaatacg gctcttcgaa acggcccaaa ttcatggagg caatactcgt
    20221 tgcaatggtg gatgatttgg atagtaaaat gaatacgatt cagactttta ttgagtccga
    20281 aagaaatgtc agtgataaat ggtctcgcta tcacgatctt tttgaccgct actttatgtt
    20341 ggataattta aaagaacgct attgaggcag catgctaggc aaagtaaaag agcttcttca
    20401 gacttataag acttacgatc cagcggcaaa atctttattg gagattgggc tgctttatcc
    20461 agggcctaaa gctctttttt atcatcgctg cgctcattgg ttttatcgcc gtcattttta
    20521 ttttatggct cgtgccattg cggagttttc tcgtgccact acgggcattg aaattcaccc
    20581 aggcgctacc gttggacggc gcctggtgat agatcatggg atgggtgtgg taattggcga
    20641 aactgcggtg attggtgaca actgtatcat ctttcatggt gtgactttag gcggaattaa
    20701 attcgatccg gtaaagaggc atcccaccgt gggtaataat gtcatgattg gcaccggcgc
    20761 caaagtattg gggcctatta ccttgggtga tggctgtcgt atcggcgcca atgcggttgt
    20821 gactaaggat gtggctgcgg ggtcgataat tgtggggcct gcttcttctg ttcttgagaa
    20881 aaggtagact cggcaatcag aattctcaga ccgactttat ttactgattt aatttttccc
    20941 gtgaacttcg atattttctt tttcaaattg tacaattgag tatccaagcg ccctgacctt
    21001 gaatggccag ggccccacac ctttgcataa atctccgatc gggagaccac ctgccgggga
    21061 ttgctgacta aacagtagag tatcttatat tcaagttgag tgaggggggc tggttcgcca
    21121 ttaatagtca ctgtctgaga tcgggggaag aggatgagat taccgatatg aatgctttca
    21181 tcttcggcaa tgcggcgccg atttttaatg tgaagaagac acttcttaac gagatcatga
    21241 ctctcaaaag gacggtttat aaagtcggct gcccctattt caaaagcatt caggcgatga
    21301 tcgtaggact tcgtatctgc gataaaaata gcaggaacaa gcccgtaatt ggttaactcg
    21361 ctaatttttt tataaagtaa gatgccggtc atatctgaaa tcgaataatc cacaatcaac
    21421 agatcaaaat tttgatgatc aagaccttcg aagattgtta aatcggtcag gctagcggag
    21481 tgagctttaa gggggtgata aatgtctgta agaagctgtg tctttccatg taaaaataat
    21541 attttataat ctttgagttt catactagat gtcatataaa tcaccattgt ttatatctat
    21601 ataaatacct ctttgtttca ttcctatcaa gtggattgat tgcgcggggc gtaggtttgc
    21661 ggtcattaag gtggctgcac tcatcgactt agaggatcgt ctaggaaatt cttcttatct
    21721 agaaatgact acaaaaatca cgtttttatt caaacactaa aagagcgaaa ttaaaggagt
    21781 tctcttttga aaaaacaaat tatgtccgca gccttaggtt tgatgcctct ttttgcatgg
    21841 gccgaattca cgccttatgc ttcgattcta gtgggtattg aaaatggaaa aagttttaat
    21901 cagggaaccg tttcagataa taacgggcaa ctgcagagtg ccacgttgat tgaagattac
    21961 ggcagcttct ttggcgttcg ggggcatgag aatcttgacc cgaatctgcg tttgatttgg
    22021 caaattgagc aatatgtgac ggtagacaat agtggctttg gcgcggacaa aaatacttta
    22081 gcgaacaaag acagttacct aggtatttta gggaacttcg gttctgttcg aatcggaaga
    22141 ctttcgacaa ttccaaaaaa tgacatggaa tacgtggatc cttgggagta tgacggaaaa
    22201 agtgttaatg gcctggcgat gttcgcgcgg ttggacaatc gattgaataa tacgcttaag
    22261 tatgagagcc ctggatattt tggagcgaag ttaatcatgc tttactctcc ggatgaggta
    22321 aaggatgtta acggcaaagc gaaggatgtt ttcaatgcgg ccctccttta caaagacgcc
    22381 acctatttcg cagcttatag ttactatatt aaaaataatg tccacaatgg tgaagatcct
    22441 gatgtttgga atcgcattga ggccggtgtg gatcaacaaa tatggatgtt gtcgttgggc
    22501 ttccaaaagg tgaatggcta tagccggagt ttggcttaca acccctccgc ctatgaggca
    22561 caaaaaagtg tgctactgac tggagcggag tccattcaag gtacagaata ttcagcgacc
    22621 ctgaactata ctttccaaga aaaattgacc tatcgtctga gctacgctca aggcatagat
    22681 atggaaataa atggcgtggg agttgatgac acaggctaca agcacatggt cttcggtgca
    22741 aattaccgtt tgtctagtaa atcaaaagcc ttcatcgatt acggccaagt gaattggaac
    22801 ggcaactatt tcggaacggg cactcgcatt acggaaaaca atttagcctt gggcttgacc
    22861 ttggatattt aaagatctct tggcatctgc ttacgagtgc ctcgcactat tttctccaag
    22921 agctcggcat tggatcttaa atttgtagac tgggaggata attcttccgc cgtcgctgcg
    22981 gcttcctcgg agactgaggc attctgttga gtgacctgat ccaattgagt catggccttg
    23041 ccgatttggc tgatgccatg actttgctct tcactcgcac tggcgatttc attatttaat
    23101 tcggcgactt ttcttacggc cccgacgatt tcggaaagaa ccaggcctcc ttgattggct
    23161 tggtgatttc ctttctctat ccgctcagaa cttttcttaa gaagttctga aatatctttt
    23221 gcggcagcag cacttttttg agctagactt ctgaccgcat cggcgacgac tgcaaagcct
    23281 tttccttgct ctccggctct tgccgcttcc accgcggcgt tgagggccaa aagattggtt
    23341 tgaaatgcga tatcatcgat aatggtggtg atttcttcga tgcgttttga atcagtcgaa
    23401 atattctgaa tggattcaat aagagcatga acttctcgtt cccctttttc cgcactttct
    23461 cgagctgcag aggcgaggat cgcggattgt tttgagttat ccgtattcag gcggaccatg
    23521 gaagtcagtt ctttcacggt cgaaacagtc tcttctaaag acaccgcttg ttgggtcgaa
    23581 gattcagaca aagattgtga ggaatcggcg attttctggg aggttgagtt tatttgccct
    23641 ccggactcat ttaagaatcc actgagtttt tgtaactctt gaattggacc tctaatagtg
    23701 aaggctgcca atagaagagc cagcagggcg cccacaccaa tgaaaatacc aagataagta
    23761 cttgaagaga tcacgccggc ctgagcttgt gccgctttaa tggcggctaa gtccgatgat
    23821 atggcaaaga ccccatgcag ttttccctct ttccaatttt ccatttgata acccagaaga
    23881 tctttgccat ttccccacgg gcttttagcg ggatcaccgt ggcagacaaa acatcccttg
    23941 gattcggtca atcttacagg acggtaaacg gtcacttttt gatcattgtc ttcaacccac
    24001 tctttgagat cgggtgtgct ttcaaatttt ttaaacactt ccatttctct cagagtcgct
    24061 aaattttctt ttcggcgtgg ttcattggaa aaaacgcgaa atgtatagtt ttcggtttca
    24121 gaaccttcgg cgcctatttt catggccgca taaataggaa cttgctggag aatgattttt
    24181 ttttcttcgt ctaaaatttg actgggatct ttatactttt gcgtgatttt ttcgatcagg
    24241 ggaggcaacc ccccttgact ggacacgtac ttcgcggcga cgtctaagcg ggaatggatc
    24301 gttctcgctt tttccatcag tcctttatga agttctttat tattaaaatg aatggaaatt
    24361 atcagggaga tcccaccaca tataaaacat gtcataaaga cagtaagtaa tattttaatg
    24421 cgaattccaa agtggtctat ccaaagcata agttcccctg cgagattcta ttcggtttaa
    24481 ttttgtgttt tgcaaagagg atggctgaaa attgactgat attttacaaa gtatagaaat
    24541 aaatattaat aaatttatat tgagacaaag agtcctgttt ataattttgt cgcccgacct
    24601 ttggccacgg cgggtgctga caaggggtct tctggccacg aatgctttgg ataacgagtg
    24661 cgcagttctt tgcgaatttc agggtaacca ttttgccaaa agctcttcag gtcactggtt
    24721 atttgcatgg gtcgataatt agggcctaag agttgcatgg tcagggcttg gactccgccc
    24781 aacagtttgg gagtctccgt ccaaccaaag atttcttgaa tccttacttc cataaaagga
    24841 tcgcgattgg caggatactg aacccgtaaa aagtttccgc tgggaacctg taattttgcg
    24901 ggcacttctg attttaaaaa ctgcgccacc tcagaactca aaagactttc aaagaagtaa
    24961 accagatctt tatgggctac agttttaaaa tctttttcac cgctggacgc catgccaaag
    25021 gcttcgtatt tttgctcctc agagaaaata tttttttcac cagcgagctc caccatgcga
    25081 accagttttt cgcggagtga atctttgctg agcttctcgc gagtctccat cagataagcg
    25141 aggcgctcca gccactgagc cagagcttca ttttctttta ggacccattc ccactcccga
    25201 gtaaggatct ggggtaaatg ctcgctgact tccttggcct gggcgatgga gacacttcct
    25261 tcctctaaag gaagatcgcg gaaatacttt tcttcgcggg aataaaactg tcccttatca
    25321 cgatcaaaag agagatcttt tttcagttct atttcgtggc cgagttcttt aaacagaaag
    25381 tctttgttaa agccggcggc taagctgacc agggtctccg ctccttgcga ggtctccact
    25441 ccatccagag ccaagaaaaa ctcactttct ttcactagac tttcaggaga aatgcgcacg
    25501 ccacggcctc cgatcattcg tgccctatcg cttccgagac ttcgccgtcg acataaacga
    25561 tcagtgaaag acctcagcaa aagtctttgc agcgccaagg acagatcaag acttcgtgag
    25621 tgtgagtgtg attgtatcgg tgcatttttc acgtaagaaa gaatttgttt tgccgcctga
    25681 tcgaccgttt gtaagccttg gggatgggtg tggcggggag ctcggccaga gcgaaattgc
    25741 tgcaaaatat ccagacgcaa caaaaggtcg ctttcatagc gatcggcgag gtagttttcc
    25801 gtagagcttt ctgttttttg cttgaccata aaatcacgct cttgcacaag agaggccaca
    25861 tcggcaccga gacgaggaac tttcatttcc accgccaaca gcatgagctt tgccaagcga
    25921 ggaggcaggg gccattgtaa aatttggcgg cccagagcgg tgatctggtt tttttgatca
    25981 agagctccta ggttttgcag attttcgctg gattttttta aagacaaggc cgcgggtttt
    26041 tcaaaccaag tgaaagaatc aaaatcgctg acaccttgag cggctaaaaa caataaactc
    26101 tcactcagct cagcgcggaa gatttccgga acgtcatggc ctggcatcga gccctcatcg
    26161 tgcttattcc acagacgata gcaaacgccc ggaaactggc gcgccgatcg ccccgcgcgc
    26221 tgttgtgccg aggccttgct gatgcggccc atctccagac gagaaaatcc tgtgcgcagg
    26281 tcaaatttca tattcttcgc caggccgctg tcgatcacgc aggagacgcc atcgatggtc
    26341 acggaagatt cagcgatatt ggtggaaagc aaaatctttc ggtgaggtcc tttttgcagt
    26401 gcttttcttt gcgcttctaa ggccaaactt ccatgcaagg ggaccagatc aatctgtttt
    26461 tcctgggccc aagtctccag ttgccgaaag gcccggtcaa tttctccgac ccccggtaaa
    26521 aaaaccaaga tgtcttcaga ggtactccgc tcggcttctt ttacatgagc caccagattt
    26581 tcaaaaaacg ctggagatgt ttgcaggagc tgcgagtttt tctgatattt tatttccaga
    26641 ggaaaaagct ttcctggaac ctccaagata ggagcttcgc tcaaatattg tgaaatttta
    26701 ggtgaatcca aagtggccga catcacgacc aacttcaaag ggctccccaa ctcttgcagc
    26761 tcgcgcaaaa gccccagggc caagtccaca tgcagagagc gttcatgaaa ctcatcgagg
    26821 atcacatact ccacgccttg aagctctgga tcctgcgcca attttcgcga gagtaaggcc
    26881 tcggtgagaa aagttaattt tgtgttggcc gaggttttgt tcgcaaagcg gacttgatag
    26941 cccacgtcct cgcccaaagt ccaagcttgc tcttcagcca cacgatgagc ggccgcaatc
    27001 gctgccattc gccgaggttc caggactaaa gttttgccct ttccatgctt ggctaaaaac
    27061 gcgggaaggc gagtggtttt accagctccc ggtgctgcgg tgatcaccag atttggatgc
    27121 gtctccagcg acttcagggc ctgcgccagg aaatcatcaa tcgggagagc gagaggtgga
    27181 gagatcggcc gagaactcaa tagttcgatc cttcaccata aagcagaatc ataaactccg
    27241 ctttttcaaa ggggagctgc ttagccagag aatcggcggt gccctcaagg agggtctcat
    27301 cggtttgggt caaattcaat gccaataaca aacgacgttt tggaaagaac tcctgcagat
    27361 cacttaaagt cttttttagg cgatagggcg tgtccatcaa tatcagaggg cgtttttctt
    27421 tttgaatatc ctgcagagct tttttacggg cttcattttc cgccggcaaa aatccacgaa
    27481 agataaactg atcaatgcgc tcgccactga gcgagagcag ccccatcagc gccgaagccc
    27541 ccagcacgga tttgacctgg atcttttttt ggcggcagag cttaaccaaa tgcgctcctg
    27601 gatcacaaaa tcctggagtg ccacagtcag agaccagcgc tgcctgtttg tgcgtgcaga
    27661 tttccgccag ggcttttact tcctctgtgg tggtatgttc attgagcagc tcataggttt
    27721 tcccagagat tccgtgggca cgcaaaagtt tgctggcttc cttcgtgctt tcacaaataa
    27781 taacttccgc cgattttaag atctccagag cgcggaggga aatctcgctg acgtcgccga
    27841 tgggggtggc taccacatac aacattattt tttcctctgc ttggtgaaga gaaagtccat
    27901 aattttgggg atatcctctg gaggcaaggc tttcccggca tgggtgcttt caaaaaagac
    27961 cacgctgact gcggggagac gctggcggag atgatgaatg tttaccggct cgttttcaaa
    28021 aaaccacatc ttggcatatt gatcttgcgg taagcccgca aaccaatccg ttttaaattc
    28081 cgcatcatcc aggcttttgt gaggcttaag caccaagcgc gtttggcgct catccagagg
    28141 aaaacccacc tgtttgaggg tctgcaccga ccctgggccc atgcgctgga tatcccttcc
    28201 ggtgaggtaa accacttccg cacctcgctt gaccacggca ttgacgtagt ctacggctcc
    28261 ctcatagggc aggtcgtggt gaaggtaatc attggaaaaa aagcgttggt accagtattt
    28321 atggagggca tcttgaaact cgggatggtg tccatccagc cccgctcttt ccagagcgtt
    28381 cttaaaaccc caatcgtttc cctgcgtgcg aatattttta agtatttgaa cttgcttagg
    28441 aaaatttact ttatggactg ggtctgcggc aaaatccatg agaatttttt ccactcgcgg
    28501 ggagacgtta aacaaggttg agtcgatgtc aaaaacgacc aagctgcgca ggccttgatt
    28561 ttgaaattct tcaatcttaa caagaatctg atgaagtaga tggctggatt gcgtcatgtt
    28621 ctgaaatcta cctttggaag ttctttaaag gcaaagactt ttcttggccc cggagcgaat
    28681 tcttgttact ttctcgcgac taaaaataag gagatggaag tatgaaaaag ggtcttcgtt
    28741 tgttggttgt tttaaccagc gcagtgattt tagcggcgtg taccggcaaa aaagctgaac
    28801 taggcacagc cgagaatccc attaaaattc actttgttcc ctctgtggat gccaaagtga
    28861 tcgaagatgg ttccaaactt ttcaaagcat ttctcgaaaa aaatacgcct tataaatatg
    28921 aaatctccat gccgcagtcc ttcgtcgccg ttgtcgaggc ctttggtact aaaagggctg
    28981 acgttgctgc tatcaatact ttcggttacg ttttagccca tgataaatat ggagctgaag
    29041 ctcgcatgac agtgattcgt cacggctttt caacttatca atctcagttt attgccaaag
    29101 cggatggtcc gatcaagagt atcgccgatc ttgcgggcaa aaaagttgca ttcgtggatc
    29161 cttcttcaac ttcgggctac cttctccctt taaaaacctt gaaaacaaaa aaaatcgaac
    29221 ctaaagagac tgtttttgcc atgaaacatg actctgtggt ttccatgatc tatcaaggtc
    29281 aagtggatgg aggagcgaca ttttactctc cgccgtcgaa agatgaaaaa ggcaacgagc
    29341 agttggaaga tgcacgccgt ttggtcctga ctcagtaccc ggatgtggaa aagaaaatta
    29401 agatcctcga actttcagag cccattccga atgatcccat catttttaga aaagacatgc
    29461 ctgaagaaat aaaagcaaaa gtcgttgatg cgattttcgc ctttgtctcc acaccagagg
    29521 gcaaagatgc ctttaacaaa atttacggcg tgactgagtt caaaaaatca acagatgcgg
    29581 actatgaacc cgttcgtaaa atgctcaatg aactcggtaa aaacgccgct gatttaatga
    29641 agaaataatt gccgttttgt tgaaggagaa catatgtctc agcccattct ctcgatcaag
    29701 aatttagtca aaacctatcc caatggagtg caagctctga agggagtcag ctttgacgtt
    29761 aaaagagggg aatttttggt cgtcattgga ctgagtggct caggaaagtc cacgctcctt
    29821 cgttgtgtaa accgtctcca tgatcctacc tcgggtcaaa tcgtctttga aggaaaggat
    29881 attgcccagg ttcaaggcga agaagttcgc agtttgcgca aacatatcgg gatgatcttt
    29941 caacacttca atttgatccc tcgccatagt gttctgagca atgttttaat gggacgtctt
    30001 ggagccatgg gatctttcca aagcatcttt ggcttctttt cggatgagga taaagccaaa
    30061 gcgttgaagt atttaaaatt ggtgggtatt gccgataaag caaaaatccg agcggatcaa
    30121 ttatctggag gacagcaaca gcgtgtggcg atcgcccggg ctttagcgca aggtccttct
    30181 atcctattag ccgacgagcc tgtggccagc ttagatcccg ccacctgtca tacagtgatg
    30241 gattatctta aaaaggtgaa tactgagctg ggtatcaccg tcgtttgtaa tctgcatttt
    30301 ttaagtttgg ttcgtcagta tgcaactcgg gttatcgccc tcaaaggcgg cgaaattgta
    30361 tttgaaggta aaccaagcga tatcacggaa gaatggttcc agaagatcta tggccagggc
    30421 tctaaagaag ttcatatcaa ctaagggtga gataaatgaa aagctacgta ttccgcagga
    30481 ctttcttcga cagttttatt tttgcctact ttgccatgat tcttttggtg gtagcagcgc
    30541 aacccactcc tgaacaaatg ctcaaccttg gccagaattt tttaatgttt gtgattgcaa
    30601 tggtcatagg cgcggctttg agtgcgggtt tgaataaatc taaaaactct tcagctcata
    30661 ccttgggtga atctcttttt gaacccgctt ataaaaaagc cgaaaaagcg gagcgtttct
    30721 ggtataaaac ttttgctggc tggcaactgg ttatcttctt aggagtgact tttatcgtgg
    30781 gtttgaagtc gaccaaattt tccgtcacgg aacttttgaa tgaagacggc tttgccggtg
    30841 cccttcgtct gtttaaggga ctcttcaatc ctaattttga aatcttaccc aaagcggttt
    30901 tgaatattat cgaaactgtt tttatggctt tcatggcaac gaccttggcg attccagtgg
    30961 cctttgtttt gagtttcttt tgtgcaaaga atatcatgaa aagtccgaag gcttatgcgg
    31021 tctatatgct cctgcgcact ttacttaatt ttactcgcgc tattgaagcc ttgatttggg
    31081 ccattatctt ttcagtatgg gtgggaattg ggcctttcgc gggaatgctg gctttgttga
    31141 ttcattccat cgcttctttg accaaacaat attctgaaat ggttgaaacg gtgagtgagg
    31201 ggcctattga aggaattcaa tccactggag cgaccaagtt tcaaaccgtg tggtttgcta
    31261 ttgttcctca ggtgatcctt ccttatattt cctttactgt gtatcgttgg gatatcaatg
    31321 tgcgtatggc gacgatcatt ggtctggtcg gggggggcgg tattggaaca atgcttatcc
    31381 agtatcaagg ccaagcgatg tggcctgaag tgggttgcat catactcgtc attgcgattg
    31441 tggtttgggg aatggatcaa gcttccgcag tgattcgtga atctttgaaa taagaaagtc
    31501 taaggtctta tggcaaatat tggcatctac aaactgctta ttcgagaaac tcatctagac
    31561 acctatggcc atgtgaataa cgccgtttat ctgacaatgt ttgaagaggc tcgctgggaa
    31621 gcgattacgg cccgaggcta tgatttcgag aaggttcatc agtcaggtca gggtccggtt
    31681 attttagaag cgaatttgaa attcatgaaa gagcttcgcc tgcgcgaaga aatcaccatc
    31741 accacggaac tcgtcgacta tccaagcaaa ataggacatc tgaagcaaca gatgatcaaa
    31801 gcggacggaa gtatcgcctg tgaggccctg tttacctttg gcctctttga tatgaagacc
    31861 cgtaaaatga tcgaagccac tccagactgg aaaaaggctg ttggccttga agcttaattg
    31921 ggacgttatt ttttgagact tgtcctttga ttatatccaa ataacgtatt ttttatcctt
    31981 tgattatatc caaaaaagat ctataatcaa aggatgtata ggactataaa taatcagctt
    32041 ctcaattgga aacaaagtaa aaaaaggaag cctctgcttc tgaggggcgc gcggcaggtc
    32101 gggaaaacct gggtcgttcg tcaattagga cagacttttg attcctatgt ggaagtaaat
    32161 ttagaaaaac aaccagagct agatcagtta tttaaagaaa actttggtaa accgaaactt
    32221 ttagttaagc aattgaacct ccttttaaat gtgagaattg aaccgggcaa aactttatta
    32281 ttttttgatg aaatccagga aaataaaaat gcgcttatat ctttgcgcta tttttacgaa
    32341 gaaatgccca acctgcacgt aattgcggcg ggatctctca ttgagtttgc tttaaaagaa
    32401 ataagctttc cggtgggcag aatccaattt cactatatgt ttccccttaa ttttgcggag
    32461 tatttaatag ctcttggcag gtcagactta ttagctgaaa ttcagcaact gcccagtgtt
    32521 aaaatatccg atggagtcca tgagaaaatt aatgaagaat taaaaacata tttatttatc
    32581 gggggaatgc cagaggttgt acaatcttat ctcgatcaaa gggatatgcg attgtgctct
    32641 caaattcaaa atgatttaat aaataatttt aggcaagatt ttaacaagta tgcaagtcgc
    32701 tctagagttc cacacctaag gctggttttt
//
LOCUS       DATBTE010000089         8417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1102877, whole genome shotgun sequence.
ACCESSION   DATBTE010000089 DATBTE010000000
VERSION     DATBTE010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 8417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8417
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102877"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VIG33_03535"
     CDS             complement(<1..300)
                     /locus_tag="VIG33_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269934.1"
                     /translation="MNGQGLPIILNLVAAFLGAVGQFFYQRGGQVLKTVPLWKNYNLQ
                     LGILSFGLVMLLFVKAYQAGGKISIVYPFYATTFVWGYFIGVYVNREPASALALAG"
     gene            complement(297..1169)
                     /locus_tag="VIG33_03540"
     CDS             complement(297..1169)
                     /locus_tag="VIG33_03540"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY8269935.1"
                     /translation="MAKQKLPRNELLTKVFTIIKGWWMIFTTIALSSLLGSWGIWTLF
                     LGLSLYSVKEYLVISQLCTSTKKTVRIFYTLGTIAFYLLLLSSHGTAFAYMFAIIFQL
                     FITTVLLLQNKNREAIPLNIAAGFGIFTLVASLSSISQVAFLGDQIWHKSGAGVAALL
                     ILIILTSLNDVFQFIGGKFLGKNKIVPHLSPNKTEAGFISGIIGTTLLGCVLLINLLE
                     ISLGKAVVLGIAISLSGILGDLFFSAVKRSTGSKDFSDLIPGHGGVLDRIDSLIFTGP
                     VFLLTLHALFQVIP"
     gene            complement(1385..1531)
                     /locus_tag="VIG33_03545"
     CDS             complement(1385..1531)
                     /locus_tag="VIG33_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269936.1"
                     /translation="MGISLSPDSYDQVKLCFDDVKNQYTIVETYKACPAEFLEQTTAD
                     PCKP"
     gene            2058..2291
                     /locus_tag="VIG33_03550"
     CDS             2058..2291
                     /locus_tag="VIG33_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269937.1"
                     /translation="MKKLITVAVFALLANVLVPSASVAFHEKFISIGFSYYTQDSLKR
                     ISKNETGTIGFLGEANYPFNLKYDYKFGADWFG"
     gene            2257..2640
                     /locus_tag="VIG33_03555"
     CDS             2257..2640
                     /locus_tag="VIG33_03555"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEY8269938.1"
                     /translation="MTTNLVQIGSANISNFDHTVLKIILAHEIGHLLGLGHSASTKAL
                     MYYDASAKTSLSLSQDDIDGISYLYPSDEMSSGKYGGCGLVTNVSPPNSNHEILVLLA
                     LLLPVLLALGLRQLSNLGLTNKLRF"
     gene            complement(2611..2964)
                     /locus_tag="VIG33_03560"
     CDS             complement(2611..2964)
                     /locus_tag="VIG33_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HEY8269939.1"
                     /translation="MNTEPKCPKCGSENTYADGSLWICPECSQEWSLHAAAVEEDAPV
                     DDAIRDANGNILNEGDSVTVIKELKIKGSSSVVKVGTKVKNIRLTDGNDGHNISCKID
                     GIGAMNLKSEFVRKA"
     gene            complement(3071..3328)
                     /locus_tag="VIG33_03565"
     CDS             complement(3071..3328)
                     /locus_tag="VIG33_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEY8269940.1"
                     /translation="MKHLILASLVSLVGFSAYAKKNCTEQPKDKWMKEADFKKKMEDQ
                     GYKISKFKQPGSCYEIYGTNKEGKKVEIYFNPVDASIVKEE"
     gene            3530..4246
                     /locus_tag="VIG33_03570"
     CDS             3530..4246
                     /locus_tag="VIG33_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY8269941.1"
                     /translation="MRQGLIYDLPTRLFHWLFSGLFLTAFIIAKTVDDESSFFSYHML
                     AGLTLGFVVILRIAWGIFGTQHARFSNFALNPSDLISYFKGILSGDKRRWAGHNPASS
                     WAAIVMMVLALGLGLTGYLMTSGPNKETFEDVHELFANAFIVVAILHVAGIALHTIRH
                     QEMIGLSMINGKKSNITAEQAIPSSRPGVGILFIGLAVVFGTYIFKNYDSQTRNLQAF
                     GTTLHLGETKGGGDHDGDDE"
     gene            4280..4792
                     /locus_tag="VIG33_03575"
     CDS             4280..4792
                     /locus_tag="VIG33_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8269942.1"
                     /translation="MNSRERGVIAAVLTIITIMVGFDLVTDAREGALGWHVLVEGIIG
                     GVALFGVFYIIRGSMELKHRLEKEIKDFSDFRKEADAWRAESRKYIDGLSLAIDQQLT
                     KWKLSSAEKEVAFLLLKGLSLKEVAEIRNTTEKTARAQSMAIYAKAGLAGRSELAAFF
                     LEDLLVPDRG"
     gene            5107..5979
                     /locus_tag="VIG33_03580"
     CDS             5107..5979
                     /locus_tag="VIG33_03580"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8269943.1"
                     /translation="MQKINPCLWFDNQAEEAAKFYTSIFESSKIGKVSHYGEAAAEAS
                     GQAKGSVMTLEFELEGGKFLALNGEPNFKFTPAVSFFVWCETESEIDALWGKLFAGGS
                     AIWELKKYPWAEKYGWCVDKYGVSWQLMLAEEKRKIAPALLFVNKLYGKGEDAINFYL
                     SVFENSKIKNIHRDPNTNAVMHALFLLEGQEFVLMEGEGSHEHVITPAISFMVNCKTQ
                     EEVDRYWEKLSHGGTTNQCGWLTDQFGVSWQIVPTFLVEMWSDLPPEKSEKVMKAMLK
                     MQKLDIAVLTKASL"
     gene            6026..7342
                     /locus_tag="VIG33_03585"
     CDS             6026..7342
                     /locus_tag="VIG33_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEY8269944.1"
                     /translation="MKKSPAGSFLFATGIENSYPTISNGRKRVDQMEKSLHYKKWSED
                     FDLVEDLGISYLRYGPPIHKTWLGIGIYDWEFTDLVYKNLADRDIVPITDLCHFGLPD
                     WLGNFQNPDFPKLFADYAGDFATRFPWVQLYTPINEMFICAKFSAYLGWWNEQEKSDR
                     SFVTALKHIVKANVLAMQAILKVRHDAIFIQSESTEYFHAENPSAIKPAEIMNAQRFL
                     SLDLNYGRRVDSEMYEYLMDNGMTRNEYHSFLGLGLKHNCIMGNDYYITNEHRVSANG
                     KTQASGEVFGYDEITVQYYNRYLLPVMHTETNLNEGPNGNEAVNWLWKQWANVLHVRN
                     KGIPMVGFTWYSLTDQVDWDTALCEDNGRVNPLGLFNLDRKIRPVGRAYKELINSWRL
                     VLPTQSVCLKVPIVPPCAYLKGWVNYQARDEQVNQYDGTSAPANRS"
     gene            7557..7994
                     /locus_tag="VIG33_03590"
     CDS             7557..7994
                     /locus_tag="VIG33_03590"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8269945.1"
                     /translation="MARVSTYLNFPRQTEEAFRFYQSIFGGEFNGGIHRFGDIPPHEG
                     APTISEADKKLVMHIELPILGGHILMATDAPESMGFKVSAGNNIYINLEPDTRLETEK
                     LFAALSSGGKIEMHLQDMFWGAYFGSCVDKFGIHWMFNCVSKS"
     gene            8078..8362
                     /locus_tag="VIG33_03595"
     CDS             8078..8362
                     /locus_tag="VIG33_03595"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEY8269946.1"
                     /translation="MEKIGPFQETAQNAWQSLHQKVSEISEQNKISGYMSLYKIEPQM
                     IYRAGVVVESKPEKLLEGFQYTQFSGGKYLRFILKGLMRICQKRAEEFLK"
BASE COUNT         2460 a         1637 c         1892 g         2428 t
ORIGIN      
        1 cccggcaaga gccagtgcac ttgcgggttc acgatttacg taaacaccga tgaaatatcc
       61 ccagacaaaa gtggttgcat agaacggata cacgatcgag atcttgccac cggcctgata
      121 agctttaacg aataacagca ttaccaaacc aaacgagaga atacccaact ggaggttgta
      181 gtttttccag agaggaaccg tctttaagac ctggcctcct ctttgataaa agaattgtcc
      241 gacagctcct aagaacgcgg ctacgagatt caaaataata ggaagtcctt gtccgttcat
      301 gggatcacct gaaaaagagc atgcaatgtt aaaagaaaca caggtcctgt aaagatgagg
      361 ctatcaattc gatccagcac gccaccatga cctggaatta aatcactaaa gtccttggac
      421 ccggtggatc gcttgactgc agaaaagaat aaatcgccga gtattccgct taaactgatg
      481 gcgatcccca ggacaacagc ttttcccagg gatatttcaa ggaggttgat caacaagaca
      541 caacccaaga gggtggttcc gataatgccg ctgataaagc cggcctctgt cttgtttggg
      601 ctcagatgtg gaacgatctt attcttaccg agaaactttc cgccgataaa ttgaaataca
      661 tcgtttaaac ttgtaagaat aatcaaaatc aataaggcgg cgacgccggc tcctgactta
      721 tgccaaatct gatcccccaa aaaggccact tgcgaaatac tactgagaga agcaactaag
      781 gtgaatattc caaatccagc agcaatgttc agtggaattg cttctcgatt tttattttgc
      841 agcaacagca cggtggtaat gaataattgg aaaataattg cgaacatata agcgaatgcc
      901 gttccatgag aggaaagcag caagagataa aaagcaatag taccaagcgt atagaaaata
      961 cgaacagttt ttttggtgga agtacaaagt tgagaaatga ctagatactc tttcacgctg
     1021 tagaggctga gtccaagaaa taaggtccat attccccaag accccaacag agaggacaga
     1081 gcaatggtcg taaaaatcat ccaccaacct ttgatgatag tgaaaacttt cgttaacaat
     1141 tcgtttcttg ggagtttttg cttcgccaaa aagacgccaa ctgtggccaa agtcaatagc
     1201 ccaaaaatcg cggttaatat ttgctgaaca gaattcatat aatacctcgg atgagtttta
     1261 cgaagtctgc aaattatatt cccggagaga gggaggctct aagccgcttg gatggggctc
     1321 attggaattc tggcaatagt cagatggctg atgagtgtac aaagatagaa tttttacaat
     1381 ttacttaggg cttacaagga tcggcagttg tttgctctaa gaattcagcc ggacaagctt
     1441 tatatgtttc cacgatggta tattgatttt taacatcatc aaaacaaagt ttcacttggt
     1501 catagctatc aggagaaaga gaaattccca tcgccttttt ctcgtcatga actccggagc
     1561 aggctccagg gccgccatga atttcagagc cttctcttgt cccaaggctg atttcagaga
     1621 agttgtgatc ttgcgatact gttagactgg ctttgccatc gtattgcccc ttgcatacac
     1681 tgagggacag agctcgtcct gtggtgttct aaatggtggc gatatcttcg acagtcttct
     1741 tacaaactac gtcgcatccg atgactaaaa aagataaagc taagcttatg gttgaaagca
     1801 gcaattttga aagcataggg cacctcacct gctcttttaa agcaagtgat gtgccaatca
     1861 atttatagat atctcaattt gagaaaatcc ttttccttac tgaagtggta ctgccttgtt
     1921 tttgagagtc atctccaatt tattttattg cctgacagag aagatgctaa ggagcagaga
     1981 ctcgaccaaa ggcaatgact gtcatttgct atcttaagat ccatgacaaa gttaagtccc
     2041 agcacaagaa tatgattatg aagaaattaa tcacggtcgc agtatttgct ttgcttgcca
     2101 acgtacttgt gccgagtgca agcgtggctt ttcatgaaaa gtttatttca attggattta
     2161 gctattacac ccaagattct ttgaagagaa tatctaaaaa cgaaactggg acaatcggat
     2221 ttttaggcga agccaattat ccttttaatc taaaatatga ctacaaattt ggtgcagatt
     2281 ggttcggcta atatctcgaa ctttgaccat acggttttga agattatttt agcccatgaa
     2341 attgggcatc tcttaggttt ggggcattct gcgagcacga aagctctgat gtattacgat
     2401 gcttcagcga agacaagttt aagtctttca caagatgata tagatggaat tagttaccta
     2461 tatcccagcg atgaaatgag cagcggtaaa tacggcggct gcggtctcgt gacaaatgtc
     2521 tctccaccta attcaaacca tgagatttta gtgttgttag ctttactgtt gccagtgctg
     2581 ctagctttgg gactaaggca actttcaaac ttaggcctta cgaacaaact cagattttaa
     2641 attcatggcg ccaatgccat caattttgca ggagatgtta tggccatcgt tcccgtcagt
     2701 aagacgaata ttttttacct tagttccgac ttttacaaca gaagaagaac ccttaatttt
     2761 tagctcttta ataactgtca cagagtctcc ctcattgaga atattgccgt tggcatcgcg
     2821 aattgcatca tcaaccggtg cgtcttcctc gactgcggca gcatggaggc tccattcttg
     2881 ggagcactca gggcaaatcc ataaacttcc gtcagcataa gtattttcag agccgcattt
     2941 tgggcattta ggttcggtgt tcacaaacta tctcctggtt agagatcggg atatatcata
     3001 aattcgtgtc accggcaaag tgaacttatg ggcgtaagtt aatacgccca ttttttgaac
     3061 ctgttttaac ctattcttct tttacaatag aggcatcaac gggattaaag taaatttcta
     3121 cctttttacc ttctttgttc gtaccataaa tctcataaca agatcctggt tgtttgaatt
     3181 tagagatctt gtagccttga tcttccattt tctttttgaa gtccgcctcc ttcatccatt
     3241 tatctttagg ttgttctgtg cagttcttct ttgcgtaggc tgaaaatcca actaaagata
     3301 cgaggcttgc aaggatcaaa tgtttcatga gtggtctcct tttagtgcta tgggttaatt
     3361 cccatactca acaaaagtat ctccttaaag tagattggca aatggattga atggcctaaa
     3421 ccatcaattt cactagacat aggacatttg tcctatggac ggacaagggt ttaatatcga
     3481 tagtgtagtt gaggcgcaca aagttattta cttagattgg agcacacata tgagacaggg
     3541 acttatatac gatttgccta ctagattatt tcactggctt ttttcgggtc tttttttgac
     3601 ggccttcatt atcgctaaaa cggttgacga tgagtcttcc tttttttctt atcatatgct
     3661 ggcaggactg accttgggct tcgtggttat tcttcgaatt gcttggggaa tctttgggac
     3721 acagcatgcc cgattttcga actttgcgtt aaacccttcg gatttaatta gctattttaa
     3781 aggaatttta tcaggcgaca aaagacgatg ggcaggacac aatcctgctt cgagttgggc
     3841 agctatagta atgatggtat tggccctagg cctgggtctg acgggttatc ttatgacttc
     3901 aggtccaaat aaagaaactt ttgaggatgt tcatgagctt ttcgccaacg cctttattgt
     3961 cgttgcaata ctccatgtcg ctggcatcgc tctgcatacg attcgccatc aagagatgat
     4021 tgggttaagt atgattaatg gcaaaaaatc taatatcact gcggagcaag cgatcccttc
     4081 gtcaagacca ggagtaggaa ttttatttat tggactggcc gtcgtttttg gaacttatat
     4141 ttttaaaaat tacgacagcc aaaccagaaa tcttcaagcc ttcggcacga ctcttcactt
     4201 aggagagacc aaagggggtg gagatcacga tggagatgat gagtagttcc aaggagtttt
     4261 taaaaatgcg gggtgtattt tgaattcaag agaaagaggg gtcatagcgg cagttcttac
     4321 catcataact attatggtag gctttgattt agtgacggat gcacgtgagg gagcgctcgg
     4381 atggcatgtc cttgttgaag gtattatcgg aggtgtagct ctttttgggg tcttctatat
     4441 cattcgcgga tccatggagc ttaaacatcg cctcgaaaaa gaaatcaaag atttttcaga
     4501 ctttagaaaa gaagcggatg cttggagagc tgaatcacga aagtatattg atggtctctc
     4561 cttagctatt gaccaacaac tgactaaatg gaaattatcg agtgcagaga aggaagtggc
     4621 cttcttatta ttgaagggcc tgagtttaaa ggaggtggcc gaaattcgca acaccactga
     4681 gaaaacagcg cgagcgcaat caatggcaat ctatgcaaaa gcaggtcttg caggacggtc
     4741 ggagttagct gcatttttct tagaggatct acttgtgcct gaccgtggtt aaatcgatca
     4801 aacaatttac tgattctgtg ctggatgaaa gtttcgggat acctggaaat taatgatgat
     4861 cgctttttaa cgatggaatc ttctcgaaca atctagacct tgctttaaac cgccacattc
     4921 tgcggcttgc ctctcctctt tgatcaaatt aaaatgtgac gattaagata ggttctaatt
     4981 ccagtagctt attactttta taaagaggaa tgaaatgact tccactctaa tcctccgtga
     5041 actgaatcca aacgatgagc ccgcttttct ccggagttat agaacctaaa atattaagga
     5101 gtttttatgc aaaaaattaa tccctgcctt tggtttgata atcaggctga agaggccgcg
     5161 aagttttata cttctatttt tgaaagttct aaaatcggta aagtatctca ttatggagag
     5221 gccgccgcgg aagcttcggg acaagcaaag ggttcggtca tgactttgga attcgaactg
     5281 gagggcggga aatttttagc ccttaatggg gaacctaact tcaagttcac tcccgctgta
     5341 tcattctttg tctggtgtga aactgagagc gagattgatg ccttatgggg aaagctcttt
     5401 gcaggcggat ccgctatctg ggaattgaaa aaatatccat gggccgaaaa gtacggatgg
     5461 tgtgtggata aatatggggt ttcatggcag ctgatgcttg ccgaagaaaa acggaaaatc
     5521 gcaccagctt tgttgtttgt aaataagctt tatggaaaag gcgaagacgc tattaatttt
     5581 tatctttctg tatttgagaa ttctaaaata aaaaatattc atcgcgatcc aaataccaat
     5641 gcagtgatgc atgctctctt tctactagaa ggacaggaat tcgtattgat ggagggagaa
     5701 ggatcacatg agcacgttat cactccggcg atttctttta tggttaactg caaaacccag
     5761 gaggaagtcg atcgatattg ggaaaagctc tctcacggcg ggaccaccaa tcagtgcgga
     5821 tggctgaccg atcaattcgg cgtttcttgg caaattgttc ctactttctt ggtggaaatg
     5881 tggagcgatc ttccacctga aaaatccgag aaagtcatga aagcgatgct taaaatgcaa
     5941 aagctggata ttgcagtatt aacgaaagcg agtttgtgac cgtcttttga tttttgtcat
     6001 actgactgaa aggagcagcc atctgatgaa aaaaagtcct gcgggctcct tcctgtttgc
     6061 gacaggaatt gaaaatagtt atccaacaat ttcaaatggt cgcaagcgtg tagatcagat
     6121 ggaaaagtcc cttcattaca agaaatggtc tgaggacttc gatcttgtag aagacctagg
     6181 tatttcatat ttaagatacg gcccgccaat tcacaagact tggctgggaa ttggcattta
     6241 cgactgggag tttactgacc tcgtttacaa aaaccttgct gatcgtgata ttgttccaat
     6301 cacggatctc tgccacttcg gacttccaga ttggttgggc aattttcaga atcccgattt
     6361 tcctaagtta tttgcggact atgctggaga ctttgccaca cgttttcctt gggtccaact
     6421 ttatactcct atcaatgaaa tgtttatttg cgcgaagttt tcagcttatc taggatggtg
     6481 gaatgaacag gaaaagagcg atcgcagttt tgtgacggca ttaaagcata ttgtgaaggc
     6541 caacgtgctg gcgatgcaag cgatactcaa agttcggcac gacgccattt ttattcaaag
     6601 tgaatctact gagtattttc atgcagagaa tccaagcgcg atcaagccgg ctgaaatcat
     6661 gaatgcccaa agatttttat cgcttgatct gaattatggt cgtcgcgtgg attctgaaat
     6721 gtacgaatat ttgatggata atggaatgac ccgaaatgaa taccattctt ttctgggatt
     6781 aggtttgaag cataactgta ttatgggcaa tgattattac atcacgaatg aacatagagt
     6841 gtcggctaat ggaaaaacgc aggcttcagg cgaagtcttt ggctatgatg aaatcactgt
     6901 tcaatattat aatcgctatc tccttccggt gatgcatacg gaaacaaatt taaatgaagg
     6961 tcctaacgga aacgaagccg tcaattggct atggaaacag tgggcaaatg tgcttcatgt
     7021 gcgtaacaaa ggtattccca tggttggctt cacctggtat tcgctgacgg atcaagtgga
     7081 ttgggacacg gctctttgcg aagacaatgg gcgagtcaat cctttggggc tttttaacct
     7141 cgatcgaaag atccgacccg tcggtcgggc ttacaaagaa ttgataaact cgtggaggct
     7201 ggtgttgccg acccagagcg tgtgcctcaa ggtgcccatt gtccctccct gcgcttacct
     7261 caaaggatgg gtaaattatc aagcacgcga tgaacaggtg aaccaatacg atggaacctc
     7321 cgcgccagcg aatagaagtt gagggggctc tagttgaagg aaaagtgaaa gggattcgag
     7381 tcaacgccgt gtttgcgaat cgacggaagc cgaatcgctg gattttagtg cgcaggaaac
     7441 ttacttgcaa agagtgttat tttaaaggat cggctgagtt gacaacaaaa atgaaaatta
     7501 tatgataaat acaaatatga ttactaatca tcgtatgaaa actgccggag gcaatcatgg
     7561 caagagtgag tacttattta aactttcctc gacaaacaga ggaagccttt agattttatc
     7621 aatctatttt tggaggcgaa ttcaacggtg gcattcatcg cttcggtgat atccctcccc
     7681 acgaaggggc tccaactatt tctgaagctg ataaaaaact agtgatgcat atcgagctcc
     7741 cgattttagg aggccatatt cttatggcaa cggatgcgcc tgaatctatg ggatttaagg
     7801 tcagtgctgg aaataatatt tatattaatc tcgagcctga tactcggttg gaaacagaaa
     7861 agttatttgc agcattgtct tccggcggga aaattgaaat gcacttgcaa gatatgtttt
     7921 ggggagctta ttttggttca tgtgttgata agtttggaat tcactggatg tttaattgcg
     7981 taagtaaaag ctaattatcc taacaatgaa tgagaaaaat catgaacttg aatgaaaaac
     8041 cggatactat ttttggcccg tatctcatta tgtctttatt gaaaaaatcg gaccttttca
     8101 agagaccgca cagaatgcct ggcaaagctt gcatcagaaa gtttcagaaa tttctgaaca
     8161 aaataaaatt tctggataca tgagccttta taaaattgaa cctcagatga tttatcgtgc
     8221 cggagttgtg gtagaatcca aaccagaaaa acttctggag gggttccaat atacccagtt
     8281 ttccggcgga aagtatttac ggtttatttt gaaggggctt atgcgaatct gccagaagcg
     8341 tgcggaagag tttttgaaat agttcatcaa acgcaagttc aaattcgcga ggatttttta
     8401 ttgaaaatta tgtcaac
//
LOCUS       DATBTE010000090        14580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_78583, whole genome shotgun sequence.
ACCESSION   DATBTE010000090 DATBTE010000000
VERSION     DATBTE010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 14580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14580
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78583"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..610
                     /locus_tag="VIG33_03600"
                     /pseudo
     CDS             155..610
                     /locus_tag="VIG33_03600"
                     /inference="COORDINATES: protein motif:HMM:NF015688.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
     gene            619..2082
                     /locus_tag="VIG33_03605"
     CDS             619..2082
                     /locus_tag="VIG33_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEY8269947.1"
                     /translation="MQKQNDLHERIQTTLKNAALLLEPIKELIRGIETNTIPWHICAG
                     WMVGSVLVLFFKIDLFAISHTRFSGLYPYHPILYKIYAFSLSTMAFWLWAWGEVKKKK
                     DKLKTLTNAFKNAGLESKIGRLPNLISDYPLDPMTRKMRLTNAGFPVSSFRAQSKYIE
                     SELGIFIDEIKENREKRAVDVIYSHYPMAEEVLYSQGSTSHSYEFLVGQTRAHTITAS
                     LRDTPHLLVAGQTGGGKSTFLRQVIVHLYIHEKACKFLLIDLKGGLEFSMFENRKQFV
                     VVPHVLAAVEQLQHINTLLDERMVFLKENQCKDIDSYFKKDKKSEKPINLSRQIVVVD
                     EAAEMFLAGHHAKSTEVQAARAILSRVARQGRAVGVHIIVATQRPDSRSLDPQVKANL
                     TGVICFQMMNDASSIAVLGNGRATDLPKIPGRAIWKNGIEMIEVQTPFLSVEEAERLL
                     GEPDDLPKEKPKNSQETAKQGVSVVDADQKLDPSLTE"
     gene            2107..2988
                     /locus_tag="VIG33_03610"
     CDS             2107..2988
                     /locus_tag="VIG33_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269948.1"
                     /translation="MKTKKRCEITNRDYKMLRFLWKWKAVSTHALARKFFPGVQSFSA
                     YRRLLHLESDGYIGSYVVDGRFHEAWILKEKGFKYILPHLGDLHSQGYKSANYPHDFL
                     ATALHLGEWLTHQPENTQTYSEQQLRCYPVDLWPSWVPRSTLHRPDGYSAYQSNEKRV
                     VIAFEAELSLKGKKRYESAVTFYDNQPAIHYVFWLVDSKNTLNAVRRNFEKLQVRDWS
                     KHQFILLSDFMQNGWMALFIEGKFKGQSPGYFLNHNPATMSSQSYLSCDTLALLDSRR
                     RPINSRSCPNWQAASKV"
     gene            3138..3539
                     /locus_tag="VIG33_03615"
     CDS             3138..3539
                     /locus_tag="VIG33_03615"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEY8269949.1"
                     /translation="METKELAAILVLPLILNACSTAPKSILMGAVVGGTAGAGLGQAQ
                     SQNSGGTAVGALVGAGLGSLIGYLAFTDKQKKDANANASAKKESPEELMPFLTRPKIR
                     SYIVPDTIEGNKYIKSHRVFILEDPGSWSKD"
     gene            3602..4969
                     /locus_tag="VIG33_03620"
     CDS             3602..4969
                     /locus_tag="VIG33_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEY8269950.1"
                     /translation="MNFQTHLGDSLEILKTIPSNSVDSLVTDPPAGIGLLGLDWDKDK
                     GGRREWVAWLTQIMTECHRILKPGAHGFVWAIPRTSHWTGAALEDAGFQVKDIITHIF
                     GSGFPKSVAIDKAIDRAKYTSTDELFKVTDWIRARRDELGLTNKQLDEIAGVKGGACH
                     WTAKLPHGQPHIPTKERWEKLEAVLGPAPDWVNSMIKPSHELGENWSKRAVVGHYKKD
                     AGGIGGVAFKSTHHTMTEPISDVSKPWQGWGTALKPASEHWILIQKPISEHNIAANVL
                     KHGVGGINIDQSRIPIQGKIPSTTNLDFRDGGFLWDAGTRSQSSVYHQHPNGRFPANV
                     IVTRSNDIDCPARIMNDQSEREVDVAPYFKNFKPEVPFLYCKKPGKDERDVFNTHPTV
                     KPLRLMRYLCKMATPSNGTVLDPFMGSGTTGVAALFEKFNFVGIERDETYHQIASKRL
                     KGVKP"
     gene            4966..5355
                     /locus_tag="VIG33_03625"
     CDS             4966..5355
                     /locus_tag="VIG33_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269951.1"
                     /translation="MSENEKTESFRPKLEHVALSAASVQKINAWLEQASAKKKGVKIS
                     RKDFINWLIEKSPDNLSGSDLSALIDRFYDEAKFLRHLLREVNQAKAEGKPKTGFELV
                     VKAKRTDTKQEVERIEASTLVLDTNDQ"
     gene            complement(5545..6537)
                     /locus_tag="VIG33_03630"
     CDS             complement(5545..6537)
                     /locus_tag="VIG33_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269952.1"
                     /translation="MHTNNFLQLVVFLVLSACQYAVAHGGMEGGGGKGVLCGTQVRVL
                     DLYEAETIHHLPIPTSQNDLDANVAHHALALGYYQSETGFDQNNASYKELIFNFYKQE
                     VIDKFSDIAPGTRLPFTPDATLPNLPSGCSFVQIALYADDGVLYRDRYYWDRLTIQDQ
                     AALILHEGIYRGRRLQGELMSDETRYIVGLTFARQLPEPIFKPMWNAPGSWWCGTKDP
                     MTERFELYAINESRNGKSGLGIYFVTFKNYFQYERTSAFVPGIAIRDLFIGKTDFSAQ
                     VHSYLTNRNWVLEYTGAYSRKDNSTKIRAWSQGDPATKSSSADCELRMTAPSLK"
     gene            6645..7457
                     /locus_tag="VIG33_03635"
     CDS             6645..7457
                     /locus_tag="VIG33_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HEY8269953.1"
                     /translation="MSKESTALLFKTIDFRTYVQAWVSARPRGEYRRISEALAMHTTL
                     VSQVFNGKKCLTEEQAANLCSYMGLNSLERDYFLKLVQIERAGSSHLKDIFLGHLKQI
                     RKQAQEIKNRVPESKELSEQDRALFYSSWQYSLIRLLTTLKGLQTAEEISGRLDLSLS
                     RVQEILSFLVSRGLCIYRDGLYSRTNQNTHVEAHSPLSIRHHQNWRTKALAMLETSSI
                     ENLSFTAPVTIAKKDVAKVKEILLETISQISKVVEDSPSEEVVYLGIDWLKI"
     gene            7481..8248
                     /locus_tag="VIG33_03640"
     CDS             7481..8248
                     /locus_tag="VIG33_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269954.1"
                     /translation="MKILLCSIRLTFIFGLTELQMSVRLNSNVLIKCENHLFDGLDWM
                     FSLLDWPTEMIDLGLKYLDSGGPMKIFWCLVASFVSISVFAKSSKECQELKSEQIRLA
                     LTGANLANLNSTHTPEGGPYRPYIVKSCSNGGCDVKRDDRAPLMKYLPDHPDADRNGY
                     VAFPNIDQKSEYATFNMTAMKLKLLALGKTCGAKVIIDNASSSFALRYQGKGSEAKED
                     IFNLNSQHQVISWMRQDFNGASNTSNFRADAEVVFYR"
     gene            8721..10577
                     /locus_tag="VIG33_03645"
     CDS             8721..10577
                     /locus_tag="VIG33_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY8269955.1"
                     /translation="MSQNLQITPKLKIGWALMRVSTKQQAEVQHGSLEQQRHMLERWS
                     LQQSEGSNSRYEITRFFEEDISGRGKAIHKRMALMEIERAIETKSIDFVVFEKVDRLA
                     RDIIYNLGLVKKAQENGVEVHEFESGQIDLRDRGSRLGFNIKNLLAEEYSLDLEEKIT
                     KKQREARINNGKDTSTVAVLGLDPHPAKRGMYVINKKEQEIVLDIFKKFIEVGSLKSV
                     AEYCKERHYVTKSRMTKEKSDKEGNIIPPQKVGGDPIDSAYLRNIFTNPKIRGFAYFK
                     DSWNQFPKMQDTEGMVRWEYPHGSVVPLDIFDEAQAVLEKNAKFNCRLRGPVYYLSSI
                     LYYEDGSKFSGAGAHGRNTEYQYYHCPKKSFRIRKDKIENFIINRVKHYLANSDILKK
                     MIDDTNAMTNMGVPILAEQVLNLRKRLTENKKVIDGFSSFVRQSALTNPSQLDSVIRT
                     ITAERDKAETETMLLERELANKENQLRELKSENQEKGMREHLEELLENFDNQEDKKKR
                     DIIQLIIQKAIVHKDNKLEIWIRRDIGSKASKNLTGCASQPDFGHECASYQPPVGMER
                     RGDLIFSNSGDSIIGSEDSEVSGVFTASSNLSRSEESGSSELKMAGWTGIQP"
     gene            11004..11330
                     /locus_tag="VIG33_03650"
     CDS             11004..11330
                     /locus_tag="VIG33_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269956.1"
                     /translation="METALSIHMPEQKRSVQMRALLLILEADSNLLKSVETFINFDTE
                     TIYWQQILKIPFGSGHRAAITWAYGIWTDEVRPNSNCFDAALSMTPNLQAGVLKALAL
                     RWGLSQ"
     gene            11389..11691
                     /locus_tag="VIG33_03655"
     CDS             11389..11691
                     /locus_tag="VIG33_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269957.1"
                     /translation="MSTQQTKLSPQMCAVLRIITADQFLSDAVFRFINIQNDSIEWEL
                     IFSLDLDLGHRAAIEFANMVWNDRLKPDSQLFEDALSMSPELQRACLKALGMRWEL"
     gene            11788..12555
                     /locus_tag="VIG33_03660"
     CDS             11788..12555
                     /locus_tag="VIG33_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY8269958.1"
                     /translation="METSTSQPKKRRTALYARVSTGNQATGLDAQIRVLRIFCEQNNI
                     ADCELLADENQSGTKASRPALDRMMKAVEEGEIENVIVFAFSRYARSVSHMLRGLEIM
                     RKNKTNFISLSEKLDLNTSLGNVVFVIISAISQLERDLIAERVRNGLAAARARGAKIG
                     RVRKRNDALIHSLLKAGLSFREIARIAKCSHGSVSASKKEYLVLKALEDKQKQEESTT
                     KFDDNQGLCMPVALLEASPDTDAKIAAAKNVGEQEKS"
     gene            complement(13060..13461)
                     /locus_tag="VIG33_03665"
     CDS             complement(13060..13461)
                     /locus_tag="VIG33_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269959.1"
                     /translation="MKFILIVSLLISANFSKADTGFTIGDFIVTKKHFHGMVVALYPD
                     QTVDVKFTFHELDGWGDRVVNLPINRVSKIPSSIDGFNVNDNVCTSSHECGRIIALYP
                     DHTVDIKVTYNEIDGWGDHVSNNSTKSILKK"
     gene            complement(13970..14317)
                     /locus_tag="VIG33_03670"
     CDS             complement(13970..14317)
                     /locus_tag="VIG33_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269960.1"
                     /translation="MKKIFVILSLLSISYQAFAEETGSSTLGDTTCQNLAVSAVQVIF
                     TIAHPNFSISGVNTKPGIKAVDGYFTKRSYQVIIDGTPLQKKDSKTGLFEYYVDFKDS
                     NGPCGSISISEAI"
BASE COUNT         4342 a         3308 c         3168 g         3762 t
ORIGIN      
        1 ggcatcggct cggctaaaac gtcgaaggcc gaggccgccc actatgcgat ggtgaatggt
       61 ccactcttaa accggctatg gtggaccaca tgggaccggt aaaccggaga aaatggcccc
      121 tttttaaacc ggcgcttcca ctgttagagc agaaactact gaggcacttc gaattcaagg
      181 cgaaatattg attcccgcag gggcaaccct gcttggcact ggccaatcaa ctgaagaacg
      241 cctgatgatt cgattcaccc aagtcgtttt caaagatggc agcttcgaaa atatccaagc
      301 tcaagctgcg gatgctgaag acaaaatagt tggtcttcgc ggatcacgag ttggaaagta
      361 tgcaatgaaa tatgcgactg ccgttggact gaattttgtt ggaggaatgg ctgagggtct
      421 tcaagatcgc gaagtcgtcg gccaacaggt cgtgacaaaa cccaacacca agaatgctct
      481 tctcaatggg gcaagcaaag ccactcttga aatggcaaat gagacgatga ccgatcttaa
      541 aaattcggct ccgataattc aaattccagc aagccaagaa attttcatta ttttcgaaaa
      601 cggccaataa gtatctaaat gcaaaaacaa aatgatctcc atgagcgtat tcaaacgacg
      661 ctcaaaaatg ctgcactact tcttgagcca atcaaagaac taattcgtgg gatcgagact
      721 aacaccattc cctggcacat ctgtgcaggc tggatggtag gctcggtgct ggtattattt
      781 tttaaaattg atctgtttgc tatctctcat acacgcttca gtggcctgta cccttaccat
      841 cccattttat acaagattta tgcgttttct ttatcgacca tggcgttttg gctatgggca
      901 tggggcgagg tcaaaaagaa aaaagataag ctcaagactc tcaccaacgc atttaaaaat
      961 gcggggcttg agtcaaaaat tggacgcctt cctaatctca tttcagacta tccactcgat
     1021 ccaatgactc gaaaaatgcg gcttactaat gctggctttc cagtctcaag cttccgcgct
     1081 caatcaaaat acattgaatc agaattggga atcttcatcg acgagatcaa agaaaatcgt
     1141 gaaaagcgcg cagttgacgt catctactca cactatccca tggctgaaga agttttgtac
     1201 tctcaaggat caacttcgca ctcctatgaa tttttagtcg gtcaaaccag ggcgcacaca
     1261 atcactgcga gtctccgaga tactccgcat ctcctggtgg ccggacaaac tggtggcggt
     1321 aaatcgacat tcctccggca agttattgtg catttatata ttcatgaaaa ggcttgcaag
     1381 tttttactca tcgaccttaa gggcggtctt gagttttcga tgtttgaaaa tcgaaagcaa
     1441 tttgttgtgg ttccccatgt tttggcagcc gtagagcagc tgcaacacat caacactttg
     1501 ctcgatgaac gaatggtttt tctaaaagag aaccaatgca aagacatcga tagctacttc
     1561 aagaaagaca agaagagtga aaaacctatc aacctatctc gacaaattgt tgtcgtcgat
     1621 gaggccgccg aaatgttcct ggcaggtcat catgcaaaat ccaccgaagt tcaagcagct
     1681 cgtgcaattt tgagtcgagt tgcaagacaa ggtcgcgcag ttggtgtcca cataattgtt
     1741 gcaacccaac gtccagacag ccgctcactt gatccgcaag tgaaggccaa tctcacaggt
     1801 gtgatctgct ttcaaatgat gaacgatgca tcaagcattg cggtgcttgg caatggacgg
     1861 gctacagatc ttccaaaaat tccaggccgc gccatctgga agaatggcat tgaaatgatt
     1921 gaggttcaaa ctccgttttt atctgtcgaa gaggctgaac gtctgcttgg tgagccagat
     1981 gatttaccca aagaaaaacc aaaaaatagt caagagactg caaagcaagg tgtaagcgtc
     2041 gttgatgccg atcaaaaact tgatccgtcc cttaccgaat agcttgctcc gttaaaaggt
     2101 aactcaatga aaaccaaaaa acgttgtgaa atcacgaacc gcgactacaa aatgctgaga
     2161 ttcctatgga agtggaaagc ggtttcaact cacgccctag ctcgaaagtt tttcccagga
     2221 gttcaatctt tctcggccta ccgacgactc ttgcatttag agtctgacgg atatatcgga
     2281 tcatacgttg tcgatggtcg attccatgaa gcttggatac taaaggaaaa aggattcaaa
     2341 tacattctcc cacacctcgg agaccttcat tcgcaaggct acaagtcagc gaactatccc
     2401 catgattttt tagcaacagc acttcatctc ggtgaatggc taacgcatca gccagaaaac
     2461 actcaaacct acagtgaaca gcagcttcga tgttatcctg tagatctatg gccatcctgg
     2521 gtgccacgtt caacacttca tagacctgat ggctactcgg cttatcagtc caatgaaaag
     2581 cgagttgtca ttgcgtttga agccgagcta tcgctcaaag gcaagaaacg ctacgagagt
     2641 gcggtcactt tctatgacaa tcaaccagcc attcattacg ttttctggtt ggttgattca
     2701 aagaatacat tgaatgcagt tcgacgcaat tttgaaaaac ttcaggtgcg tgattggtcg
     2761 aagcaccagt tcattcttct ctcagacttt atgcaaaatg gttggatggc tctgtttatc
     2821 gagggaaaat ttaaaggcca aagccctggc tattttttga atcacaatcc cgccacaatg
     2881 tcgtctcaat cctatctcag ctgtgacacc ctggcacttc tcgattcccg cagaagacca
     2941 atcaattcaa gatcctgtcc caactggcaa gccgcctcaa aagtctgaca taatcaagcg
     3001 gtctaaagtc cgcttacccc cataccccct tcaataccca tctcatcccc aaagcccacc
     3061 tcctacaatt tcaaattcac tcgctcaaaa attaaaaaac ccaaaacctt aaatatataa
     3121 agaaaggatg tgtctgtatg gagactaaag aactggctgc gattctcgtg ctgcccttga
     3181 tattgaatgc gtgttcgact gcgccgaaat caattttgat gggagcagtt gttggaggta
     3241 ctgctggggc aggattgggg caagcacaat cgcagaactc cggcggcact gctgttggcg
     3301 ctcttgtcgg tgcgggtctt ggctcactca ttggctactt ggcgtttact gacaaacaaa
     3361 aaaaagatgc caacgcgaac gccagcgcca agaaggaaag ccctgaagaa ctgatgccat
     3421 ttttaacccg accaaaaatt cggtcttaca ttgtgcccga tacgatcgag ggcaataaat
     3481 atatcaaatc tcatcgagtc ttcattctcg aagatccagg ctcatggtcg aaagactgag
     3541 cgccatttag tactcccact atttcaagag ccacgaagtc cctctttaca aaggactgcc
     3601 cgtgaatttt caaactcacc tcggtgactc cctagaaatt ttaaaaacga ttccctcaaa
     3661 ctctgttgat tcgcttgtga ctgatcctcc tgctggcatt ggccttcttg gccttgattg
     3721 ggacaaagac aaaggcggta ggcgcgaatg ggtcgcatgg ctcacacaga ttatgaccga
     3781 atgccatcgc attctaaaac ctggtgctca cggatttgtc tgggccatcc ccagaacttc
     3841 gcactggaca ggagccgcgc tagaggatgc tggctttcaa gtgaaagaca tcatcaccca
     3901 tatctttggg agcggctttc ctaaaagtgt cgcgatcgac aaagcaatcg atcgagcaaa
     3961 atacaccagc actgatgagc tattcaaggt gactgactgg atcagagccc gacgcgatga
     4021 acttggactc accaataaac agcttgatga aatcgcaggg gtcaagggtg gcgcttgtca
     4081 ttggactgct aaactcccgc atggccaacc acatatccca acaaaagaac gatgggaaaa
     4141 attggaagca gtcctgggtc ccgcgcctga ttgggtcaac tctatgatca aaccctcaca
     4201 cgaactcggc gagaactggt ctaagcgtgc tgtcgtcgga cactataaaa aagatgctgg
     4261 cggaatcggt ggcgtcgcct tcaaaagcac tcaccataca atgaccgagc ctatttcaga
     4321 tgtttcaaag ccatggcaag gatggggcac agccctaaaa cccgcaagtg agcattggat
     4381 tctaattcaa aaaccaattt ctgaacacaa tatcgcagct aatgttttga agcacggagt
     4441 tggtggcatc aacattgacc aatcacgcat tccaatccaa ggaaagattc cgtcaactac
     4501 caatcttgat tttcgtgacg gaggatttct ctgggatgcc ggaacccgca gtcagagttc
     4561 cgtttaccac caacacccta atggtcgatt cccagccaat gtcattgtga ccagatcaaa
     4621 tgacatcgat tgtcccgctc gaattatgaa tgatcaaagc gaacgcgaag ttgacgtcgc
     4681 gccatacttc aagaacttca aacccgaagt gccttttctt tactgcaaaa aaccaggcaa
     4741 agatgagcgc gacgtcttca acactcatcc gaccgtgaaa ccgctgcgcc tcatgcggta
     4801 tttgtgcaag atggcaacgc catccaacgg cacagtcctg gaccccttca tgggctctgg
     4861 gaccacaggg gtggctgctc tgtttgagaa atttaatttc gtcggtatcg agcgcgacga
     4921 aacctaccac caaattgcaa gcaaaagact aaaaggggtc aaaccatgag tgaaaatgaa
     4981 aaaactgaaa gttttcggcc caagcttgaa catgtagcgc tgtcagcagc gtctgttcaa
     5041 aaaatcaatg cgtggcttga gcaagcgagc gccaagaaaa agggagttaa aatctctcgc
     5101 aaagacttca tcaactggct gattgaaaaa tcacccgaca acttgagcgg tagcgacttg
     5161 tcagcactga ttgatcgctt ctatgacgag gcaaagtttc ttcgccatct gctgcgcgaa
     5221 gtgaatcagg ccaaagcaga agggaagccg aagactgggt ttgaactagt tgttaaggct
     5281 aaacggacag atacaaaaca agaagttgaa agaatcgagg cctcaactct tgtgctcgat
     5341 accaatgacc aataggccga tattttatcc tgatacaaat tctaaaaaag caattttttg
     5401 taacaaaatc tgcacattcg cgaaaaaccc tggcaattat cgcagaaaaa ttccgtccaa
     5461 ataattttat tttttctgtt cctaatggtg ctgacaataa tcagcaccat tttagaaaat
     5521 atttttctcg gccaaaacgc agcattactt gaggctcgga gcagtcattc tcaattcgca
     5581 gtcggcggaa gaagacttgg ttgcaggatc accttggctc caggctcgaa tttttgtgct
     5641 gttatctttt ctagaatatg cccctgtgta ctcaagtacc caattgcgat tggtaagata
     5701 gctgtgtact tgggccgaaa agtcagtttt gccgatgaat aaatctcgta tcgcgattcc
     5761 tggaacaaat gcggaggtgc gctcatactg gaaatagttt ttgaaagtaa caaaataaat
     5821 gccgagacct gactttccgt tacgtgattc attgatcgca tataactcaa atctttcagt
     5881 cattggatct ttggtgccac accaccatga gcctggagca ttccacatcg gtttaaagat
     5941 cggctccgga agctgccgcg caaatgtcag tcccacgatg tatcgagttt catcagacat
     6001 taattcacct tgaagtcgtc taccccgata aattccttca tgtaagatga gcgccgcttg
     6061 gtcctgaatc gttaagcgat cccagtaata gcggtcacgg tagagaactc catcgtcggc
     6121 ataaagggca atttgcacaa aggaacagcc gcttggcaga tttggcaaag ttgcatcggg
     6181 cgtgaacgga agtcgagtgc ctggtgcaat atctgaaaac ttatcgatca cttcttgctt
     6241 atagaaattg aaaataagtt ctttgtaaga ggcattattt tggtcgaatc ctgtctctga
     6301 ctggtagtaa ccaagggcca atgcgtggtg tgcgacgttt gcatcaaggt cattttgact
     6361 ggtcggtatt ggcagatgat gtatcgtctc agcctcgtaa aggtcgagga cacgaacctg
     6421 agttccacag agcacacctt taccgccgcc gccttccatg cctccatgcg ccactgcata
     6481 ttggcatgct gatagtacta agaacaccac aagttgtaag aagttattgg tatgcatact
     6541 taattccctc ccgttttttt atctttgtca ctttgatagt ttcggaggta tgcagcagaa
     6601 aaaatagatt ttgttaatct aatgaactaa gataaagttg acctatgagt aaagaatcta
     6661 cggcccttct ctttaaaacc atcgattttc gaacttatgt tcaggcctgg gtgagcgcac
     6721 gtcctcgggg agaataccgt agaatttcag aagcattggc tatgcatacg actcttgtca
     6781 gtcaagtgtt caacggcaaa aaatgcttaa cggaagaaca agccgccaat ctttgctcct
     6841 acatggggtt gaactcattg gagagagact attttttgaa gcttgttcaa attgagcgtg
     6901 ccggatcttc tcatttgaag gacatattcc taggtcatct gaaacaaata cgaaaacagg
     6961 cgcaagaaat aaaaaaccgc gttccagagt caaaagaact cagtgaacaa gatcgagccc
     7021 ttttctattc aagttggcaa tacagcctta ttcgccttct caccacttta aaggggcttc
     7081 aaactgccga agaaatttcg ggccgtctag atctgtctct ttcgcgcgta caagagatcc
     7141 tctcgttttt agtttcacgt ggcttatgca tctaccgcga cggactctat tcaagaacga
     7201 accaaaatac tcatgtggaa gctcattcac cgctctcgat tcgacaccat cagaactgga
     7261 gaactaaagc cttggctatg ctagaaactt catcgatcga aaatctaagt ttcacggctc
     7321 cggttactat tgcgaaaaag gatgtcgcta aagttaaaga aatactatta gagacgattt
     7381 ctcaaatttc aaaagtggtt gaggactctc catcggaaga ggtggtttat ttgggaattg
     7441 attggcttaa aatttaagct tacttggccg cttcggatag atgaaaattc tgctttgctc
     7501 gataaggctt acttttatct tcggactgac ggaacttcag atgtcagtac gtctaaattc
     7561 aaatgtatta ataaagtgcg aaaatcattt gtttgatggc ctggactgga tgttttcact
     7621 actcgactgg cctactgaaa tgattgatct gggattaaaa tatttagatt cgggaggtcc
     7681 tatgaaaata ttttggtgcc tagtggcttc attcgtttcc atatctgttt ttgctaaatc
     7741 ttcaaaagaa tgtcaggaat taaaatctga acagattaga cttgccctta ctggagcaaa
     7801 cttagcaaat ttgaattcaa ctcatacgcc agaaggtggc ccctatcgcc cttacatagt
     7861 taaatcgtgt tcaaacggtg gctgcgatgt aaaaagagat gacagggctc cgctaatgaa
     7921 atacctgcct gaccatcccg acgctgacag aaatgggtat gttgcatttc cgaatatcga
     7981 tcaaaaaagc gaatacgcga ctttcaatat gacggctatg aaattaaaac ttctcgcatt
     8041 aggaaaaact tgtggagcta aggtaattat cgacaacgca tcttcttcgt ttgcccttcg
     8101 ctatcagggc aagggatcag aagctaaaga agatattttc aatctgaata gtcaacatca
     8161 ggtcatttct tggatgcggc aggattttaa cggtgcgtcc aacacatcaa actttagggc
     8221 ggacgcagaa gtcgtttttt accgctgatt tcagcataca aaaccctggt attcggatcg
     8281 aagcctatca aaaaaattcg gcttagttaa aatcccatgt gcacctgcac atagcccttt
     8341 ttatattcga tttcacatca agagcctcga ctccccagca cacggagtta ttctatggcc
     8401 aagccaattg ccgttgatac ccacgggcga atttgcaatg gatgcggagc tttcaaatct
     8461 tgggagagtt tcgacaaaaa aagtgacggt ctgaatggtc gacatagcca atgcaaaaaa
     8521 tgcgtcggga aattcaaacg caaatggtgg aagaaaaaga atacgaggaa gcgagtaaac
     8581 ccaacggtcc tagaattttc gaaatctgat atcaccgaaa cttttgtgcc cttcaacagt
     8641 cacgaaaaaa cagagctcga aaaaatctta cgctcgatgg tgcttgattg cttctgttct
     8701 aaaaacggag gtcataaacg atgagccaaa atctgcaaat aacacccaaa cttaaaatcg
     8761 gctgggcgct gatgcgagtc tcgacaaagc aacaagccga agttcagcat ggctctctgg
     8821 agcagcaacg ccacatgctt gagcgctggt cacttcaaca aagcgaaggc tcaaacagcc
     8881 gttatgaaat tactcgattt tttgaagaag atatcagcgg tcgcggtaag gccattcata
     8941 aacgtatggc attgatggaa attgaacgtg ccatcgaaac caaatccatt gattttgtgg
     9001 tgtttgaaaa agtggaccgt ctggctcgag acattatcta caacttaggc cttgttaaaa
     9061 aagctcagga aaatggagtt gaggtccatg agtttgaatc aggtcaaatt gatcttcgag
     9121 atcgcggaag ccgacttggt ttcaacatca aaaatttgct tgcggaagaa tacagtctcg
     9181 atctggaaga aaagatcaca aaaaagcagc gcgaagccag aatcaataac ggcaaagata
     9241 cttcgaccgt tgccgttttg gggcttgatc cccatcctgc aaagcgcggt atgtacgtca
     9301 tcaacaaaaa agaacaagaa atagtcctag acatattcaa aaagtttatt gaagtcggga
     9361 gtttgaaatc cgtcgccgaa tattgcaaag aacgccacta cgtcactaaa agccggatga
     9421 ccaaagaaaa gtctgacaaa gaaggcaaca ttatcccacc acaaaaagtt ggtggcgatc
     9481 ccatcgattc ggcctacctc cgaaatatct tcaccaatcc aaaaatcaga ggttttgcct
     9541 acttcaaaga ttcttggaac cagtttccga aaatgcaaga tacagagggc atggtccggt
     9601 gggagtatcc ccatggttct gttgttccac ttgatatttt tgatgaagcc caagcagtgc
     9661 ttgagaagaa tgcaaaattt aattgccgat tgagaggtcc ggtctattac ctaagcagca
     9721 ttctctacta tgaagacggc tcaaaattta gcggagccgg tgcccatggt cgaaatactg
     9781 agtaccagta ttatcactgc ccgaagaaaa gttttcgaat cagaaaagac aagatagaaa
     9841 atttcatcat caaccgagta aagcattact tggcaaactc tgacatcctg aaaaaaatga
     9901 ttgatgatac gaacgctatg acaaatatgg gagttccgat tttagcagaa caggttttaa
     9961 acctgcgaaa acgtttgacc gaaaacaaga aagtcatcga tgggttttca agttttgtca
    10021 gacaatccgc cctgacaaac ccaagccagc ttgattcagt gatccgaaca atcacagctg
    10081 agagagacaa agccgagacc gagacaatgt tgttggagcg agagcttgct aacaaagaaa
    10141 accaactccg cgaattaaaa tctgagaatc aagaaaaagg aatgcgagaa catctcgaag
    10201 aacttctgga aaattttgac aaccaggagg acaaaaaaaa acgggatatc atccaactca
    10261 tcattcagaa agccatcgtc cataaagaca acaaactcga aatctggatt cgacgagata
    10321 taggaagcaa agcctcgaaa aaccttacgg gctgtgcctc ccagccagat tttggacatg
    10381 agtgtgcctc ctaccaaccg ccggttggca tggaacgaag gggtgattta attttttcaa
    10441 attctgggga ttcaatcatc gggtctgagg attctgaagt ctctggggtc tttaccgctt
    10501 cctctaatct gtcacgctct gaggaaagcg gttcgtctga actaaaaatg gcggggtgga
    10561 cgggaataca accctaggct tacgcaagca gtcgaaatga ctaaaataat atttattaac
    10621 aagagcttac cggaaactcc taaaggaact gaagtgaacc aaaatgcgcg aaaatagcct
    10681 ctcctgtccc gctgcgggac agctacggga cgctttgcgg gacagccgct agcggaacca
    10741 atcgctcccc gccaacttgt tttcaaagag aaaaccaaag gaggctcatg ggatttattc
    10801 gcttttattc agctattatt aggattggca tagggctcgc ccttctcggg cagctcaagt
    10861 tttgtactac taccatgatg gggctcgccg ctgagagttc ccaacgtgga attatttctt
    10921 attcaaaatt ctcaagacta cttaccaaat aacatggcgc tccccgttca tccgggggcc
    10981 gctaattcct atggagttaa attatggaaa cagcactctc aattcacatg ccagaacaaa
    11041 aacgctcagt ccaaatgcgg gccttacttc tcatccttga ggcagattct aatctgttga
    11101 aatccgtcga aacctttatc aattttgata ctgaaacaat ttactggcag caaattttaa
    11161 aaataccttt tggttccggc caccgagccg ccatcacgtg ggcttatgga atttggacag
    11221 acgaagtacg tccaaactcc aattgcttcg acgcagctct tagtatgact cccaacttac
    11281 aggctggggt tctcaaagcg ttggcccttc gatgggggct tagccaataa cgagttttta
    11341 aaccctctaa atcacgccag tctcgcgact ggaattttag gagaaaccat gtctactcaa
    11401 caaaccaaac tttcacccca gatgtgcgcg gttctgcgaa tcatcacagc ggatcaattt
    11461 ctcagcgatg ccgtctttcg atttatcaat attcaaaatg acagcatcga atgggagtta
    11521 attttcagtc tggatcttga tctaggccac cgggccgcga ttgagttcgc gaatatggtt
    11581 tggaatgatc gactcaaacc ggacagtcaa ctgtttgagg atgctctcag catgagcccg
    11641 gaacttcagc gggcgtgtct caaggccctc ggcatgcgct gggagctctg acaccggacc
    11701 attcacaagt cttttcaaac aattccacaa acttgcccga ggcgaacact ttcctcgggc
    11761 tttttatttt aaatgaggtt acaatatatg gagacttcta cttcccaacc aaaaaaacgc
    11821 cgcacagcac tttatgcaag agtttcaacc ggaaatcaag caaccggcct cgatgcacag
    11881 atccgagttc ttagaatttt ttgtgagcaa aataacatcg cagattgcga actcctcgcg
    11941 gatgaaaatc aaagtggcac caaggcttct cgccccgcac ttgatcgaat gatgaaggct
    12001 gtcgaagagg gggaaatcga aaacgtcatc gtttttgcat tctcacgtta cgctcgttct
    12061 gtatcgcata tgcttcgagg ccttgagatt atgcgcaaaa ataaaacaaa ttttatttcc
    12121 ttgagcgaaa agttagatct caacacgagt cttggaaatg ttgtttttgt aatcattagc
    12181 gcaatttcac aactagaaag agatttaatc gcggagcgtg ttcgcaatgg attggcggcg
    12241 gctcgcgcta ggggggccaa gatcggaagg gtcagaaaaa gaaacgacgc cctaatccat
    12301 tcgcttttaa aggccgggct ctcattccgc gaaattgccc gcattgcaaa gtgttcacac
    12361 ggctctgtgt ctgcgtcgaa aaaagagtac ctcgttttaa aagcactcga agacaaacag
    12421 aaacaagaag agagtacgac aaaattcgat gacaatcaag gactttgcat gccagtagca
    12481 cttcttgaag cgtcgccgga taccgatgca aaaatcgctg ccgctaagaa tgtaggagag
    12541 caagaaaaga gctaaggtaa taaaaatcga tttgagtcgc gaggagcttc acttggtctt
    12601 atcgcacgcc tcactgatcg gcaagattca gtcgcacgaa caatggcggc gtcctggtca
    12661 tgccaggtga ggcaaatttc tggagaaccg tgggctcaaa gaatattgac aaactcgttg
    12721 aaaaaatgaa ctgcaaagat accgtaattc aatttcagga ttatcagtca tttcgaaaat
    12781 ctcaatcgca gataacaaaa ataggggcga ctctccacta acacggaggt cgcccttttt
    12841 tttggtcacg tgtaaacaaa acgcgtgtct tcttgaaggc gccccagtat agcggttttt
    12901 tcgcaggttt cattaaaaaa taccctcgac agttccctat ggggatatcg tggggacaag
    12961 taaaatgggc aatcactcaa cgttagaaaa atcgtccgcg accagcccca ggaacaagag
    13021 ataaatggcc agagagttta tttttcggaa aaaccacctc tattttttta aaatactttt
    13081 agttgaatta ttactaacat ggtctcccca gccatctatt tcattgtagg taaccttaat
    13141 atcgacggtg tgatcagggt aaagcgcgat gattctgcca cattcgtggc ttgacgtgca
    13201 gacattgtca ttgacattga atccatcgat cgaagatggt attttcgaaa ctctatttat
    13261 aggtaggtta accacccgat ctccccagcc gtctaattcg tgaaaggtga atttcacgtc
    13321 aaccgtttgg tcggggtaaa gagcaacaac cattccatga aaatgttttt tagtgacaat
    13381 gaagtcacca atagtgaatc cagtatctgc tttcgaaaaa tttgcagaaa ttaacagcga
    13441 aacgattagt ataaacttca taacggccct tctttccaaa agaaatcaat gggctattgc
    13501 gcaaaacaaa tacagtcaac tacttttcta gcggtcactt aataaatgtc tatgggcttg
    13561 attgtggtca gcctgacaca tgcttactgg tctgatgatt ttgatactga gaagccagtt
    13621 tcacatggtc ctacccaaca ttattcgcca ggacgtctag atcgcgggca ttagtttaca
    13681 gatagctcaa ctagaaattt taaggaggga cctgacccat acgatttttg aagcgtatga
    13741 acaattgtcg acgtatttcg agccaaggcc gaagcttggc ccggttctta ttgcagagat
    13801 taatgacatc caaatttgcc gggatatcta gtgcaaaaat cggccattta atccctctca
    13861 ttttcaggtt cacaaatgaa ttctaccaga agatatagat ccgcaccttg gtcgtctctg
    13921 atgtgggctt tgattcgagc gcttacggcg aaagcaccat aggtttcagt taaattgctt
    13981 cggaaatgga aatacttcca catgggccat tggaatcttt aaaatcaacg tagtattcaa
    14041 aaaggccagt tttcgaatcc ttcttttgca ggggcgtgcc atcgataatc acttgataac
    14101 ttctttttgt aaaataaccg tcaactgctt ttattccagg cttagtgttt actcctgaaa
    14161 ttgaaaaatt tggatgagct atggtgaaga taacttgcac ggcagatact gctagatttt
    14221 gacaggtcgt atctccgagt gttgatgatc cagtttcttc tgcaaaagcc tgatacgaaa
    14281 ttgatagaag gcttaaaatc acgaatattt ttttcataat gtcctcatct ttaaaaaatt
    14341 atttaattat ttatatttaa aatgcttacc agacctataa ggtcgttatc tatgccggac
    14401 tactcgttct attttttttc taaatatatt ttctccctaa aatcatcatt ggcataatta
    14461 ataggagtaa cagaaagaat ggttccgctc atgtctcttg atactatcat gagataactt
    14521 gaactatcgt aagcagaggc ctttgtttgt aaactgaatt ttgagtttaa atattggcca
//
LOCUS       DATBTE010000091         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1171783, whole genome shotgun sequence.
ACCESSION   DATBTE010000091 DATBTE010000000
VERSION     DATBTE010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171783"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VIG33_03675"
     CDS             complement(<1..543)
                     /locus_tag="VIG33_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase"
                     /protein_id="HEY8269961.1"
                     /translation="MQEQNKGSFFDPKTILAVVLVGAVWFGWQSYLTKKYPQYNKVKT
                     VETTPTMSTPGVTTTPGENVSSTVETKRTPTPQIPEKEYQYHDQNITFTISSRGMGLK
                     NLILNQYVDYDKNPIKLGQSEDAPLFEMKLAAEQTPLNFEFKEESAGSYVGTAQVGES
                     TVTRTLKYNAEKQAFTNKIVI"
     gene            complement(527..829)
                     /gene="yidD"
                     /locus_tag="VIG33_03680"
     CDS             complement(527..829)
                     /gene="yidD"
                     /locus_tag="VIG33_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY8269962.1"
                     /translation="MRNLRKQWTRVLRPFENLPKAFLLLLVGAYRTFGTTHMGGACRF
                     TPSCSEYAVKAIKSHSLFPAIYLITKRILKCRPGGTYGIDPVPACSCSGVSDARTK"
     gene            complement(1132..1281)
                     /gene="rpmH"
                     /locus_tag="VIG33_03685"
     CDS             complement(1132..1281)
                     /gene="rpmH"
                     /locus_tag="VIG33_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEY8269963.1"
                     /translation="MKRTYQPSNRRRKSTHGFRARMATKAGQDVISRRRAKGRKRLTV
                     STGGK"
     gene            1485..>2204
                     /locus_tag="VIG33_03690"
     CDS             1485..>2204
                     /locus_tag="VIG33_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEY8269964.1"
                     /translation="MDINNSFWAHIKTKMKSRNMNNKYLETWLDPIEYLSTGGTSERP
                     RIVLGVPNALHQYYVTQNLQDQIYTEISDSYKSPFEVEFVITGNKPPLPPENPSALEE
                     AIHANEQIQLRPNSSPSQNIQGPTHKSTSDFLNTELTFSTFVVGKNSEFAHAACFNVA
                     RNPGADDYNPLYIFGPVGMGKTHLLNAAGNYIREQFPHLKITYISAERFLNECVAALR
                     HYQMDKFRQKYRENSDILLVDD"
BASE COUNT          621 a          477 c          430 g          676 t
ORIGIN      
        1 aatcactatt ttattagtaa atgcctgttt ttctgcattg tatttcaaag tgcgagttac
       61 tgtactttct cccacttgcg cagttcccac gtaagaacct gcagactctt ccttgaactc
      121 aaaatttaaa ggagtttgct cagctgcaag cttcatctca aataagggag cgtcctcgga
      181 ctgccctagt ttaataggat ttttatcata atctacatac tgattaagga tcagattttt
      241 taaacccatc ccccggctac tgattgtgaa cgtaatattt tgatcgtgat attgatattc
      301 tttttccgga atttgaggtg tcggagtcct tttcgtttca accgtggagc taacattttc
      361 ccctggagtt gtagttacgc caggtgtact cattgttgga gttgtctcaa cagttttaac
      421 tttattatat tgaggatatt ttttcgtaag ataagattgc cacccaaacc acaccgcacc
      481 cactagaaca acggcaagaa tagttttggg atcaaagaaa gaacctttat tttgttcttg
      541 catcggacac tcctgaacac gaacaagcag gaaccggatc aatcccatag gttccaccag
      601 gtcggcattt taaaattctt tttgtaataa ggtagattgc cgggaaaagg ctgtgagatt
      661 tgatggcttt cacagcatat tcagaacagc ttggcgtaaa gcgacaggct ccacccatgt
      721 gagtggttcc aaaagttcta taggctccaa ctaataatag aagaaaggct ttaggcagat
      781 tttcgaaggg tctcaagacc cgcgtccatt gctttcttaa attcctcaaa cttgagtccc
      841 ttgtaaaagc cctgatcgat gggtttaaag ataatgttaa tggaaacgtc aatcggtatc
      901 cctgcttgat tagactttat aactaagtcc ctaaagtatt cccggaccca gcgttttaac
      961 ttattgcgca ccacagaaca accaactttg cggctcgcag taacgccata ctttaaacct
     1021 ttttgatcta ccttcgagta gttaaaaagg agccattttg tgggccaggt tctttttcca
     1081 gtctttttga gagagaaaaa ttcggaactt cgtttaattg ctgtggatct actattttcc
     1141 accagtagaa accgttaaac gtttccttcc ttttgcgcgg cgacgagaga taacatcttg
     1201 tccagctttg gtagccattc ttgctctgaa accatgggtg cttttacgac gacgattgct
     1261 tggttgatat gtacgtttca tttttcaact cctagtgaaa cacatttttg acgctttggg
     1321 acgtctacca ctaaaaatag gtggatgcag aattaatcac aatgcagcaa taaggtcaac
     1381 tgttgaaaac atcgacaggc cctgctaaag cttttttggc gtttctgctc tatatgtgaa
     1441 cacgcgcgta ggtgggctta ggctcgaaag taggggtacg tcacgtggat atcaataatt
     1501 cgttttgggc tcacattaaa acaaaaatga aaagccgtaa catgaataat aaatacctgg
     1561 aaacttggct agacccaatt gagtatcttt ctacaggtgg tacgtcagaa aggcctcgta
     1621 ttgttcttgg ggttcctaat gctcttcatc agtactatgt tactcaaaat cttcaggatc
     1681 aaatttatac tgaaatttca gatagttata aaagtccttt cgaagtcgaa ttcgttatta
     1741 cgggaaataa gccccctctt cctcctgaaa atccctctgc attagaggag gccattcacg
     1801 ctaatgaaca aattcagttg cgtccaaata gctccccttc ccaaaacatt caggggccta
     1861 ctcacaaatc cacttcagat tttttgaata cagaactcac cttttcaacc tttgtcgttg
     1921 gaaaaaattc agaatttgcc cacgctgctt gttttaatgt ggcaaggaac cctggcgctg
     1981 acgattacaa cccgctctac atcttcgggc cagtgggaat gggaaaaaca catcttttaa
     2041 atgccgcagg aaactatatt cgtgaacaat ttcctcatct taaaatcact tatatatcag
     2101 cagagcgttt tcttaatgag tgcgttgctg ctctgcgcca ttatcaaatg gacaagttcc
     2161 gccaaaaata ccgggaaaat tccgatatct tattagtaga tgat
//
LOCUS       DATBTE010000092         1769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_215462, whole genome shotgun sequence.
ACCESSION   DATBTE010000092 DATBTE010000000
VERSION     DATBTE010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215462"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VIG33_03695"
     CDS             <1..252
                     /locus_tag="VIG33_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8269965.1"
                     /translation="FLKDKRLEAGLSQGAVAKKLGYTSPQFISNWERGLSQPPVVTLR
                     KIAQIYNISPNDMFEVMLKATIDQVTAELTEKFYKQKVK"
     gene            317..622
                     /locus_tag="VIG33_03700"
     CDS             317..622
                     /locus_tag="VIG33_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8269966.1"
                     /translation="MQIDVIFPNQNRHVRHLLGLYINRKRNELGFSAKEIASQVNLSP
                     HAYRRIEVGKVKINSSLFEKIHSLLFLEIEDLAEIHKIASVKYINDVSKALLENYPA"
     gene            619..1521
                     /locus_tag="VIG33_03705"
     CDS             619..1521
                     /locus_tag="VIG33_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269967.1"
                     /translation="MKNKPSLSTNKSDRLRELIRELGLPLRKLPDIISLNPEECLAWW
                     SNQEIHLKIGYTPFSRLTSLVGIGENQLFTGDYDQDLARKRLMGDHGSLPTRYQENQN
                     SFLRTSAHIIRYITLTRGQWFADQILYALNVSPLIYENPDRHINLTYFADLLELLSKK
                     DFSQEELDTLSSIIFLSLQQTTLGQSFKASENFFDIYTTLAKNFDYFDSTFEYESHFV
                     GKKYILKTVLHLDQHSQLKENPQSLRRLMRYRQILLAWFPYLGGMAPLFPKTEILRFS
                     DVLEVQYELDLTPSSKQPARLLVV"
     gene            complement(1490..>1769)
                     /locus_tag="VIG33_03710"
     CDS             complement(1490..>1769)
                     /locus_tag="VIG33_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269968.1"
                     /translation="NESYNASRWVESIGSSPKGLIFEHPAVKSPHMMMLVENFNMQHF
                     SKVYDEYKELINETYEVFPTSANKEFTDSLPTYCAELFSKPLVTVRAA"
BASE COUNT          536 a          390 c          341 g          502 t
ORIGIN      
        1 tttttaaaag acaagcgatt agaggcagga ctttctcagg gggctgttgc aaagaaacta
       61 ggctatacta gcccgcaatt catcagtaac tgggaacgcg gtctctctca accacctgtg
      121 gtgactctcc gtaaaatagc tcaaatttat aatatttcac caaacgatat gtttgaagtt
      181 atgctgaaag cgacgattga ccaagtcacc gctgaactca ctgagaagtt ctacaaacaa
      241 aaagtgaaat aaagattaaa tattcaagtt aataatacta ctctgacgct tgagctatca
      301 tcgaggacat aaggatatgc aaattgatgt catttttcca aatcagaatc gtcacgtaag
      361 gcatttgctc ggattgtaca ttaaccgtaa gcgaaatgaa ttgggttttt ctgcaaaaga
      421 aattgcatct caagtaaatc ttagtcctca cgcatatcgc agaattgaag tcggaaaagt
      481 gaaaatcaat tccagcctct ttgaaaagat ccactctctc ttgtttttag aaatagagga
      541 ccttgcggaa attcataaga ttgcaagtgt gaagtacatt aacgatgttt ctaaggccct
      601 gcttgagaat tatccggcat gaaaaataaa ccgtctctct ctaccaacaa atcagacaga
      661 ttgcgggaac tgatccgcga actcggtctg cctttgcgaa aactcccgga tattatctca
      721 ttgaatcccg aggagtgctt agcttggtgg tcaaaccaag aaatccatct taaaatcggc
      781 tataccccct tcagccgttt gacgagcttg gtggggattg gtgaaaatca actttttacg
      841 ggcgactatg accaagatct cgcacgcaaa aggctcatgg gggaccatgg ctccttaccc
      901 actcgatatc aggaaaatca aaacagcttt ttacgaactt ctgcccatat tattcgctat
      961 attacactta cccgcggtca gtggtttgca gatcaaattc tttatgcgct taacgtatca
     1021 ccgcttatat atgaaaatcc cgaccgccac atcaatctga cttattttgc ggatctcctt
     1081 gaactcttat caaagaagga cttttcacag gaagaactgg acactttatc aagtattata
     1141 tttctaagcc tgcagcagac aactctgggt caaagcttta aagcctctga aaatttcttt
     1201 gatatttata caacgctcgc caagaatttt gactacttcg attcaacctt cgaatatgaa
     1261 agtcattttg ttggaaaaaa atatatctta aagaccgtac tgcacttaga tcagcattcg
     1321 caactaaagg aaaatccaca aagtctaaga cgtctcatgc gttatcgtca gattttgtta
     1381 gcctggtttc cttacctggg cggaatggcg cccttgtttc cgaaaactga gattctgaga
     1441 ttctccgatg tccttgaagt acagtacgaa cttgacctaa caccctcttc taagcagccc
     1501 gcacggttac tagtggttta gagaatagtt cagcacaata agttggaagg gaatcggtga
     1561 actctttgtt tgccgaagtt ggaaagactt cataagtctc atttatcagc tccttatatt
     1621 cgtcatacac cttcgagaaa tgctgcatat taaagttctc cacgagcatc atcatatgtg
     1681 gggacttcac tgctggatgt tcaaaaatca gtcccttcgg agaggacccg atgctttcca
     1741 cccaacgaga agcattatag ctttcattc
//
LOCUS       DATBTE010000093         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1240623, whole genome shotgun sequence.
ACCESSION   DATBTE010000093 DATBTE010000000
VERSION     DATBTE010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240623"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1507
                     /locus_tag="VIG33_03715"
     CDS             200..1507
                     /locus_tag="VIG33_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8269969.1"
                     /translation="MNVEGRNQLYLQGKFDSPGLFPSLETSLQQPVVFELDFGLKELP
                     IEPGLIIVRGPRQFGKSTWLEKELKTSLIQFGAGSSFYLNGDFYRTHEDLYNAISDVH
                     ALFSKSVPVRRLFVDEITAIKDWEKAIKKLYDEGTTKRTLVITTGSQAVGLRRGSERL
                     PGRKGKLDRTTYRFLPVSFSEFEKKCQSYFGGKTLMAYLMCGGSPIAANELVQTKRIP
                     PYIIELTKDWILGECSRQGRDAGLLQFLLTMLHLKAMTPISMTKLAKESNAANNTIVQ
                     AYLELMRDLMSVSTCYQADPHTLRPIPRKAAKFHFINGLAALAFDPDQPWTLEEFCAQ
                     TPTKLGKWYEWAVASHIWKKSAYLGEMDPESQFFWSNDHHEIDFISPQQWYEVKSGGF
                     SPTEFLWFPKSFPRRTLKVISSVPLEVSPSFMTTESLETLLRS"
BASE COUNT          466 a          336 c          376 g          500 t
ORIGIN      
        1 agggggtgga gaaaattaaa atcccaaacc aaagaagtgt tttcatagcc tttgcatcgg
       61 catttggtcg agaaactgaa ccctaagccc taggactaaa gtaaaaccgg atcaaggtcc
      121 ggtaagaagg taatctcttt ctggtttgaa ctattatttt cttgatctca acgttcataa
      181 cgtttagagt ttaaacgtta tgaacgttga gggtcgcaac caattatatt tacaaggtaa
      241 atttgattca ccagggcttt ttcctagttt ggaaacgagc cttcagcagc cagtggtatt
      301 tgagctggat tttggattga aggaattgcc aatagagccc ggccttatca ttgtccgtgg
      361 gcctcgccag tttggtaaaa gtacatggct tgagaaagag ctcaagacgt ctttgattca
      421 gtttggcgct ggcagcagtt tttatttgaa tggcgacttt tatcgtactc atgaagacct
      481 ctacaatgcc atttctgatg ttcatgcttt gttttccaag tcagtccctg tgcggcgtct
      541 ttttgtagat gaaatcacgg ctatcaaaga ttgggaaaaa gcgattaaga agctgtatga
      601 tgaaggtact accaaaagaa ctttggtgat aacaaccgga tcacaagctg taggtttacg
      661 taggggcagt gaacgtttgc caggaagaaa agggaaatta gatcgaacca cctatagatt
      721 tcttccggtt tctttttccg agtttgaaaa aaaatgccaa tcttattttg gtggcaaaac
      781 attaatggct tatttaatgt gcggaggttc gccgattgcc gccaacgagc ttgtacaaac
      841 gaaaagaatt cccccttata ttattgaact gacaaaagat tggatattag gggaatgcag
      901 tcgtcagggc agggatgctg ggcttcttca gtttcttctg acaatgcttc atcttaaagc
      961 catgacgccg atttcgatga ctaaacttgc aaaggaatcc aacgccgcta acaatacgat
     1021 cgtacaggct tacttagagc tcatgcgaga tctgatgagt gtttcaactt gctatcaggc
     1081 ggacccgcat actcttcgcc ctattccccg caaagctgca aaatttcatt ttatcaatgg
     1141 gcttgcggct ttagcatttg accctgatca gccatggact ctggaagagt tttgcgctca
     1201 aacgccgaca aaactgggca aatggtacga gtgggcggta gcttcacata tttggaaaaa
     1261 atccgcctat ttgggggaaa tggatcctga aagccaattc ttttggtcca atgaccacca
     1321 tgagattgat tttatttcgc ctcaacaatg gtatgaggtg aaatccggcg gcttttcgcc
     1381 gaccgaattt ttatggttcc ctaagagctt tcctcgacga actttgaaag tcatttccag
     1441 tgttccatta gaggtttctc cctcttttat gaccactgaa agcttagaaa cattattacg
     1501 ttcataggtt tgctgttgag ttgattctca gtagattatc aattagtatc cataagttaa
     1561 aaattcttat caatgagagc ttccgaggct aacaaagggc gatgacactt ctccattacg
     1621 ttcagaatca ttttaaaact gctgacttgg aaattataga tttggctggg tctccggc
//
LOCUS       DATBTE010000094        11304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1240712, whole genome shotgun sequence.
ACCESSION   DATBTE010000094 DATBTE010000000
VERSION     DATBTE010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 11304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11304
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240712"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..256)
                     /locus_tag="VIG33_03720"
     tRNA            complement(181..256)
                     /locus_tag="VIG33_03720"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(221..223),aa:Ala,seq:ggc)
     gene            393..896
                     /locus_tag="VIG33_03725"
     CDS             393..896
                     /locus_tag="VIG33_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="organic solvent tolerance protein"
                     /protein_id="HEY8269970.1"
                     /translation="MRLLAVFLSNLFCVLLCTSLFSNVASAKEMVNRLGIGVKNDTSI
                     NTPALSVIYWPAPDIALTGGLGIDTEKDSSRFAMSGGVHRIVFKEDNMNFFMGGELGV
                     INREVAADKQSGFYLSGLFGAEFFFAGLENLSFSFQGGVGVVSLKDVRFRTIADTPFQ
                     SAVTFYF"
     gene            933..1310
                     /locus_tag="VIG33_03730"
     CDS             933..1310
                     /locus_tag="VIG33_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY8269971.1"
                     /translation="MKKIISTDQAPKAIGPYSQAVQMGSFLFCSGQIPIDPKTNEVFT
                     GDIKTQTEMVIKNIEGVLKAADLNFSHIVKTTIFLTNMADFAVVNEVYGKTFSSEPPA
                     RSTVAVAALPRGVHVEIEVLAHR"
     gene            1369..1896
                     /locus_tag="VIG33_03735"
     CDS             1369..1896
                     /locus_tag="VIG33_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY8269972.1"
                     /translation="MVLALASLFQFSAEYRKVIDEPMSSWTRTQSADCAVVLTGGPSR
                     VREGFDLLANQYVKKLIISGVNPNSRLREIMPVWSFYGNLNENDVVLEKRSETTYGNA
                     QQSLSIVETLHCRDIILVTSRSHMHRAFKTFRGAFPENIEIFKHAVVSGHYEVGFWEA
                     SFEALKSLFYSLWAY"
     gene            1945..2781
                     /locus_tag="VIG33_03740"
     CDS             1945..2781
                     /locus_tag="VIG33_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8269973.1"
                     /translation="MESIWAYFDSKICTLGRSVTKNIEYTLRVLLMVYLSVRAAVLDQ
                     AQGLRTIVNVILAQIYFTGWQALPLVSVMALTTGSVLILQSMTNLSLLGGTQMIGNFL
                     IVMVVREAGPLLVALVVVARSGTAVASEIGNMRANREIEALESMGINPLSFIVFPRVV
                     GGVISVVCLALYFNVLALIGGFFITKFLQDMPFSFYLDSLMRGFAEEDVIIFFAKNSF
                     SGMIIFVVSCYQGLSVKKSPHEVPQVTTQAVVNSVIYVMVFNLTVTALFYLNQLRNLG
                     VV"
     gene            2781..3524
                     /locus_tag="VIG33_03745"
     CDS             2781..3524
                     /locus_tag="VIG33_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8269974.1"
                     /translation="MSSLSKSHLSVEKIESIKFEALTFAHEGHSPLLQNADFEFPMNE
                     IVWVKGAEGQGKSSLLQVIAGLLMPQSGFFFINEKNVLDMSFEEFLPYRLAIGYTFDY
                     GGLISNRTVYDNLMLPLAYHKLVPTAEAVARVEGVVKTFGLEKFARERPAHIPGRVRK
                     LVCLLRSLVMQPQVLVMDDPSVGLGQDTLYTFVDYIHKMRTEGYLKHIFMSSYDEKYM
                     GLFNYQIIHIDDGQVYLQTVDHSKKVVHL"
     gene            3521..4495
                     /locus_tag="VIG33_03750"
     CDS             3521..4495
                     /locus_tag="VIG33_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY8269975.1"
                     /translation="MKVKFNKIERVAGLFLLFAIIGVLVTAVSVAVKQGWFATKIYYS
                     SVFENADGIRPGTLVQMAGLHAGAVEDVELQTDNKIKINFYVLSKFEPRIRQDSTAQL
                     IRPFIIGDRALEVSVGSESLPLVPPHHVLASNETLDIMSLLSGKALGVHLAKLSSTLE
                     NLAHMVEALTDKARTQSLVRIFDRLDPLLMSIEVMATEMTKLSRQVTYKGNLQTVVSN
                     LSVTTHELNAILPDLNRENPELAKDIAQMSKNLASLTSDFKVLGPAIKSVGPELPQAT
                     RRAVEALNETVIMLKAAQKSMFMRGNVQEVREEEALQRKPAHKEESKP"
     gene            4425..5783
                     /locus_tag="VIG33_03755"
     CDS             4425..5783
                     /locus_tag="VIG33_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY8269976.1"
                     /translation="MFRKFEKKKLSNGNLLIKKNPSLEKAVVKSTLASHNGLMLVIDF
                     FSHFIFSKRAGALVKRLAWLSVGAIALSITAFLVVLFVMNGMNNSIHKRILSLEPHLY
                     VSVNGISKGSVLELHPVFQRLKENSVNKVYVYETQDVILRTMDGQFHGAIARGVSEES
                     LKFMVEQLHQMEDPKRHENVQSWVPEDLPGKSEVVVGVDLARSLGVFEGDYLTIVPPE
                     GLLLPPGESPKFEKVKVSKIISTNLSDLDSQFVFYQRGTTLNTLASAASRKVGIEVWT
                     PDGKNLNDLKDEVEKFSDVMAETWMDRNSVLFFALKLEKLVIGMFLGLAGLIAATSIL
                     SVLALLMSQKKRDIAILKTLGLSDKHTIKIFTQMGMMLAAWGLIPGVILGTAISFYIE
                     KNPVNILPDIYYDSQIPALVDPKLIWGVLGVGFLVCLAGAWYPARTSSEIQPAQALRI
                     KN"
     gene            5926..7239
                     /locus_tag="VIG33_03760"
     CDS             5926..7239
                     /locus_tag="VIG33_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269977.1"
                     /translation="MHDQKSKPQVLPYSIPFLFKGLQKALVVFLMIIGVGGWSRAQTS
                     AVSLQLTPLKVKQLVLNENLSLLQAYMRVQQAEDKVMKARLDILPSINVAAALSGPAG
                     LGAQAMATLPFLLPSNWFQLNVRKDLREAEVAGMKSLALNTVLGAERSYFTVLRDQEL
                     QILLTNYKANIVEVGRRYEVRTTKHRVIDPVGVNYIRGKVRSIESALLSSQQLVDIEK
                     AALNRMITQRTETSLLKMHMAPSSLESLSSTELMNHLATEVSVSPEMQQIHWLIEASK
                     NSKWASVFAFLNSASVGTSINSSGFGNLSGTGSMNLGLGILPLFSLGDHQVGEFEYQK
                     RELSEEAWRIIQTLSATIQSTKQQILLLEDASVSYEESFRLLMARMDRGEGDYGSPLG
                     SLELSLEARIQLIDARLSLDSARLNMNRLMRMDLFQNVAFQTPKP"
     gene            complement(7283..8110)
                     /locus_tag="VIG33_03765"
     CDS             complement(7283..8110)
                     /locus_tag="VIG33_03765"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY8269978.1"
                     /translation="MSLANSIQHIWNEVQAGAKIDDLSSEKKSAIEQCIQNLDSGSLR
                     VCEKIEGTWTTHEWLKKGILLYFRLQKMSLMNAGDFNYFDKIPVKRWTGSEGVRSVPH
                     ALARRGSFIESGAILMPSYVNIGAYVGAGTMVDTWATVGSCAQIGKNVHLSGGVGIGG
                     VLEPIQASPVIVEDNAFIGSRCIVVEGALIEEGAVLGAGVTITASTKIIDVTGAQEKI
                     YQGRVPKNSVVIPGTVMKKFAAGEYGTPCALIIGQRKPSTDMKTSLTDALRDFQVSV"
     gene            complement(8112..8768)
                     /locus_tag="VIG33_03770"
     CDS             complement(8112..8768)
                     /locus_tag="VIG33_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY8269979.1"
                     /translation="MKTSVFGYSSLGLPIIAYEFRNSGPEVLILGGVHGDEVEGVFAA
                     QALLNHFMRDFSYHLNLVIVPQFNIEGVVNKTRGNAKGIDLNRNLKTKDWSPEVKTPR
                     YHPGPFAGSEPENQALMEYIDEKKPQWILSLHSWHPILNVNGDCHGEAEVLARLTGYK
                     IDADIGYPTPGCLGSYAGLERNSPTLTYEIQRGQDTAEILKIHVPAILEALKVTEKRK
                     "
     gene            8816..10033
                     /gene="lysA"
                     /locus_tag="VIG33_03775"
     CDS             8816..10033
                     /gene="lysA"
                     /locus_tag="VIG33_03775"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEY8269980.1"
                     /translation="MSKTKLYLQYENQKLVLGPQKKALDCLTKNYERPVYVYDLDILK
                     ERFQLLQKALPGVQVYYAMKANSHPEVLRCLHELGSGADVVSGGEIRCASEAGFKPQQ
                     MIYSGVGKSHKEIRQSLGLNLAQINVESIAELRRIASIAKELGQKPAVVFRLNPDVDI
                     ETHPYIATGLHNNKFGMALSDLPELKEVLYANASFLDFRGVSLHLGSQMHNLSGFRDA
                     LKLLRPVYEDLQKEFSSVDTFDVGGGLGIFYETQELAAEEKLLADYATIIFEELKGLR
                     AKIQTEPGRWLVAHAGVLLTQIQYLKKTSTKNFLIVDTGMHHLLRPTLYGAHHRVWTL
                     KESPGGGSAGGVMKKYDVVGPICESGDFLATDRELCEMNEGDFLVIGDVGAYGFVMRS
                     DYNLQDPPDEVVL"
     gene            complement(11000..>11304)
                     /locus_tag="VIG33_03780"
     CDS             complement(11000..>11304)
                     /locus_tag="VIG33_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269981.1"
                     /translation="DDNRIAADSLIDLIKINGLTDEHAEILLDWLTSKNKNFQLSAIY
                     AVCMIYRHWKTIRPDYVAVNHNFERIDSAVHKMDDSDVSLVKWIKLYYELKDAAAA"
BASE COUNT         2874 a         2307 c         2794 g         3329 t
ORIGIN      
        1 gcagaaatac attccctggg ttccttccgg gatcgaaccc cgcaggagat ccgctggttc
       61 tacgggctgg gtgcctgcag aaattttgaa actacaggaa tcgaaccgcg acactttcag
      121 tgtcccgttc ttcttccttg gaatgtttct gtaagattgt atcgacatcg tagaacataa
      181 tggtggacct gatcgggatc gaaccgacga cctcttgcat gccatgcaag cgcgctccca
      241 gctgcgctac aggcccacta aagtactttt acattaattc ttatatgact cactatttct
      301 taatccgagg ttattttaga ggcaagagtt atttaaactt gagtttagtc ctggacttcg
      361 actttaatag acaactgttc aaggagataa atatgcgttt gttagctgtt tttttaagca
      421 atttgttttg cgttttgctt tgcacttctc ttttttcgaa cgtggcctct gcgaaagaga
      481 tggtgaatcg tttggggatt ggggtgaaaa atgacacttc aattaatact ccggctttgt
      541 cggttattta ttggccagca ccagatattg cgttgactgg tggtttggga attgacacag
      601 aaaaggacag ctctcgcttt gccatgagtg gtggggttca tcgcatcgtt ttcaaagaag
      661 ataatatgaa cttcttcatg ggcggggagc tgggagttat caatcgggaa gtggctgcgg
      721 ataaacagtc cggtttttat ctgagcggtc tttttggcgc ggagtttttc tttgcggggc
      781 ttgaaaatct cagcttcagt ttccaagggg gcgtgggtgt tgtgtccttg aaggatgtgc
      841 gctttagaac gatcgcggat acaccttttc aatctgctgt gactttctat ttctaattcg
      901 agtttaagcc atactaatag gggagtattc ttatgaaaaa aattatttcc acagatcaag
      961 cgcctaaagc gatcggtcct tattcgcaag ctgtgcaaat ggggtctttc ttattttgct
     1021 ctggacaaat tcctattgat ccaaaaacaa atgaggtgtt caccggcgac attaaaactc
     1081 aaacagagat ggtgattaaa aacatcgaag gagttttgaa agccgccgac ttgaacttca
     1141 gccacatcgt aaagaccact attttcttaa ccaatatggc ggattttgca gtggtgaacg
     1201 aagtttacgg taaaactttt tcttctgaac ctccggcacg ctctacggtg gcagtggctg
     1261 ctttgcctag aggggtccat gttgaaattg aggtcttagc tcaccgctaa gagagttctg
     1321 gaaaatttaa aaaaaatcct tattcgaaaa aaaatctttt gggccttggt ggtcttggcc
     1381 cttgccagtc tttttcagtt tagtgctgaa tatcgtaagg tgatcgatga acctatgtct
     1441 tcgtggactc ggactcaaag cgctgattgc gcggtggttt tgacgggtgg gccgagcaga
     1501 gtgcgggaag gtttcgatct tctggcaaat caatatgtaa aaaaattaat tatttctgga
     1561 gtcaatccga actcgcgttt gcgtgaaatc atgccggtgt ggagttttta tggaaatctt
     1621 aatgagaacg atgtcgtcct ggaaaagcgc tccgaaacta cttatggcaa tgcccagcaa
     1681 agcctttcca ttgttgagac ccttcattgt cgtgacatca ttttagtgac ctcgcgttcg
     1741 catatgcatc gagccttcaa gacttttaga ggggcttttc cggagaatat tgaaattttt
     1801 aaacatgctg ttgtttcggg gcactatgaa gtcggcttct gggaagcttc atttgaggca
     1861 ttaaagtcac ttttttattc tctttgggct tactaattga cttaagtccg tatttttttc
     1921 tgagcccgta gccgatataa agacatggaa agtatatggg cgtatttcga ttcgaagatc
     1981 tgcactctcg gtcgatctgt tacaaagaac attgaataca ctttgcgcgt cttattgatg
     2041 gtttatcttt ctgtaagagc cgccgtactt gatcaagcgc agggtttgcg cactattgtg
     2101 aatgtgattt tagcgcaaat ctattttacc ggttggcagg cgctgcctct tgtttcggtg
     2161 atggcgctga cgacgggaag tgttctcatt cttcagtcca tgacgaattt aagtttgttg
     2221 ggaggaaccc aaatgatcgg gaatttcctg atcgttatgg tcgtccggga agccggccct
     2281 ttgcttgtag ctctcgtggt ggttgcccgc tctggaactg cggttgccag cgagatcggc
     2341 aacatgcgcg ccaatcgtga gatagaagcc ctcgagagta tgggaatcaa tccgctaagc
     2401 tttattgttt tccctcgggt tgtgggggga gtcatcagcg tggtgtgtct ggctttgtat
     2461 tttaatgtct tagccttgat tggaggtttc ttcatcacta aatttttgca agacatgcct
     2521 ttttcttttt atctcgattc tttgatgcga ggttttgcgg aagaagatgt gattatcttt
     2581 tttgccaaga acagttttag cggaatgatt atttttgtgg tctcgtgcta tcaaggcctg
     2641 tccgtgaaaa aaagccccca cgaagttcct caggtgacca cgcaagcggt ggtcaacagc
     2701 gtgatttatg tcatggtctt taatctgaca gtgacggcac tattttattt gaatcaattg
     2761 cgaaatttag gagttgtcta atgtcgagtc ttagcaaaag ccatctttca gtggaaaaaa
     2821 ttgaatcaat aaaatttgaa gctttgactt ttgcccacga ggggcacagt cctttgttgc
     2881 aaaacgcgga ttttgaattt cctatgaatg aaatcgtttg ggtgaagggg gcggaaggtc
     2941 agggtaaatc cagtcttttg caagtgatag ccgggctctt gatgccgcaa tcagggtttt
     3001 tctttatcaa tgaaaaaaat gtcctggata tgtccttcga agagttttta ccttaccgtt
     3061 tggctattgg ttatactttt gactatggtg gtctcatcag taatcgcacg gtttacgaca
     3121 atttgatgtt gcccttggct tatcataaat tggttcccac ggcagaggcc gtcgcgcgcg
     3181 ttgaaggtgt tgttaaaacc tttggtcttg aaaaattcgc ccgcgaaagg cccgcgcaca
     3241 ttcctggacg cgttcgtaag ctggtctgtt tattacgctc gctggtgatg caaccacagg
     3301 ttctggtgat ggatgatcct tccgtggggc tgggacagga cacactttat acctttgtgg
     3361 attacattca taaaatgcgt acagaaggat atctcaagca tatttttatg agttcttatg
     3421 atgaaaagta tatgggtctt tttaactatc aaattattca tattgatgat ggtcaggttt
     3481 atcttcagac cgtggatcac agcaagaagg tggttcacct atgaaagtga aattcaataa
     3541 gatcgaaaga gtggctggtt tgtttttgct tttcgcaatc attggagtct tggtgactgc
     3601 cgtgagtgtg gctgtcaaac aaggttggtt cgcgactaaa atttattatt cttcggtttt
     3661 tgagaatgcg gatggaatcc gtccgggcac tttggtgcag atggcgggtc tgcatgcggg
     3721 ggccgtggag gatgtggagc ttcagactga taataaaatt aaaataaatt tctatgtgct
     3781 cagtaaattt gagccaagga ttcgtcagga tagcacggcc cagttgattc gtccttttat
     3841 tattggtgat cgtgcgctgg aggtctcggt gggatctgag tctttgccgc tggttcctcc
     3901 gcatcatgtg ctcgcttcca atgagacttt ggacattatg agtttattga gtggaaaggc
     3961 tttgggcgtt cacctggcca aactgagttc cacattggag aatttagctc atatggtgga
     4021 ggcgcttacg gataaagctc gtactcaatc tttggtgcgc atttttgatc gtttggatcc
     4081 cttgttgatg agtattgaag tcatggccac agagatgacg aagctaagtc gtcaagttac
     4141 ttataagggc aatttgcaaa ccgtggtaag taatctctcg gtaaccacgc atgagttgaa
     4201 tgcgatcttg cccgatttga atcgcgaaaa cccagagctc gcaaaagata tagcgcaaat
     4261 gagtaaaaat cttgcaagct tgaccagtga ctttaaagtt ttaggcccgg ctataaaatc
     4321 cgtagggcca gaacttccgc aagcgactcg tcgggcagtg gaagccttga acgaaacagt
     4381 gattatgctt aaagcggcgc aaaaaagtat gtttatgcgc ggcaatgttc aggaagttcg
     4441 agaagaagaa gctctccaac ggaaacctgc tcataaagaa gaatccaagc cttgaaaaag
     4501 ctgttgtaaa gtcaacgctt gcgtcccaca atgggcttat gctcgtcatt gattttttca
     4561 gtcattttat tttttctaaa cgtgcggggg cgttggtcaa gcgtcttgct tggctttctg
     4621 tgggagcgat tgctctgagt atcaccgcct ttttggttgt tttatttgtc atgaatggca
     4681 tgaacaacag cattcataaa cgcattcttt cgctggagcc tcatttgtat gtgtccgtga
     4741 atgggatcag taagggctcc gttctggaac ttcatccggt ttttcaaaga cttaaagaaa
     4801 attctgtgaa taaagtttat gtctatgaaa cccaagatgt gattctacgc acgatggatg
     4861 gacaatttca tggggctatc gctcgagggg tgagtgaaga gagtttgaag tttatggtgg
     4921 agcagctcca tcaaatggag gaccctaagc gccatgaaaa tgttcagtct tgggtgccag
     4981 aagatcttcc cggtaagagt gaggtcgtgg tcggcgtgga tttagctcgc tccctagggg
     5041 tttttgaggg cgattatctc acgatagtcc ctccagaggg tttgttgttg cccccagggg
     5101 agtcgccgaa atttgaaaag gtgaaagtca gtaaaattat ttcaacgaat ctttcagatt
     5161 tagattctca gtttgtattc tatcaaagag gaaccacttt gaatactttg gcttcagcgg
     5221 cgagccgcaa ggtgggcatt gaggtttgga cccctgatgg gaaaaatttg aatgatctca
     5281 aagacgaggt cgaaaaattc agcgatgtca tggctgagac gtggatggac cgcaattcag
     5341 ttttattttt tgcgctcaag cttgagaaat tggttatagg aatgttctta ggtcttgcgg
     5401 gcctcattgc tgccacctct attttatctg tgttggccct tttgatgtct cagaaaaaaa
     5461 gagatatcgc gattttaaaa accttggggc tttccgataa acacaccata aagattttca
     5521 cccaaatggg aatgatgctg gcggcttggg ggcttattcc cggagtgatt ttgggcacgg
     5581 ccattagttt ttatatcgag aaaaatcctg tgaatatttt gccggacatc tattatgact
     5641 cacaaattcc cgctctcgtg gatccgaaac ttatctgggg agtcttgggg gtgggctttt
     5701 tagtttgcct cgcaggcgcc tggtacccgg ccagaacttc cagtgagatc cagccagctc
     5761 aggcgttgag gattaagaat taattaaaac tccggtcagg actcggttct gtgagatttt
     5821 aataatgaaa attatcattc aataaaagta ataatttgaa tttatcgatg aattctttta
     5881 gaagacttgc cagacacggt aacaaccctg acaagtagag cgattatgca cgatcaaaaa
     5941 agcaagccgc aagtgcttcc atactccatt ccttttcttt ttaaaggcct ccagaaagct
     6001 ttggttgtct tcttgatgat aataggggtg ggggggtggt cacgcgcgca aacaagcgcg
     6061 gtttcgctgc aattaacacc tttaaaagta aagcagctcg tgctgaatga aaatctgagc
     6121 ttgcttcagg cctatatgag agtccaacaa gcggaagaca aggtcatgaa ggctcgcttg
     6181 gatattcttc cctcgatcaa tgtggcagcg gcgctgtctg gccccgcagg tttgggggct
     6241 caggccatgg ccacgctccc ttttttgctg ccaagtaatt ggtttcaact caatgtccgt
     6301 aaagatctcc gtgaagccga agtcgctggg atgaaaagcc tggcattgaa tacggtattg
     6361 ggggcggagc gaagttattt tacggtgctt agagatcaag aattgcaaat tttactgact
     6421 aattataaag cgaatatcgt tgaagtgggc cgtcgctatg aggtgagaac cacaaagcat
     6481 cgggtgattg accctgtggg ggttaattat atccgcggaa aagttcgatc gatagagtcc
     6541 gccttgttaa gtagccagca gttggttgat attgaaaaag ctgcattaaa ccgaatgatc
     6601 acgcaaagga cggagacaag tcttttgaaa atgcatatgg cgccgagcag tttggaaagt
     6661 ttgagttcga cggagcttat gaatcactta gccacggaag tctcagtctc tcctgagatg
     6721 caacagatcc actggctcat cgaggccagt aagaattcta aatgggccag cgtttttgca
     6781 ttccttaact cagccagtgt ggggacgtcc atcaatagta gcggattcgg caacctttca
     6841 ggaacgggat caatgaattt gggtttgggg atccttcctc ttttctcttt gggcgatcat
     6901 caagtagggg agtttgaata tcaaaagaga gagctttccg aagaagcttg gcgtattatt
     6961 caaactctct cggccaccat ccagtccaca aaacaacaaa tcctcctgtt ggaagacgca
     7021 tctgtcagtt atgaagaatc ttttcgcttg ctgatggcgc gaatggatcg gggggaaggt
     7081 gattatggct cacctctcgg ttccctcgag ttgagtttgg aagcccgaat tcagctcatt
     7141 gatgcacggc tgtccttgga cagcgctcgg ttgaacatga atcgactgat gagaatggac
     7201 ttatttcaga atgtcgcttt tcagacgcca aagccgtaaa tgttttttga agcaaggacg
     7261 gaagcttttc cgtcctgttt ttttaaaccg aaacttggaa atctcttaaa gcgtcggtca
     7321 gtgaagtctt catatcagta cttggttttc tttggccgat gatcaaagcg caaggtgtgc
     7381 cgtattctcc ggcggcgaac ttcttcatga cggtgccggg gataaccact gagtttttcg
     7441 gaacgcgtcc ctggtaaatc ttttcctgag caccggtgac atcgataatc ttcgtggagg
     7501 cagtgatcgt cacgccagct ccgagaacgg ccccttcttc aatcaaagct ccttcgacga
     7561 cgatgcaacg gctgccgata aaggcattgt cttcaacgat gacgggactg gcttggatgg
     7621 gttcgagaac acctccaata cccacgcctc ctgacaagtg gacgttcttg ccgatctggg
     7681 cgcaactgcc gacggtggcc caagtatcga ccatggtgcc tgcaccgaca taggctccga
     7741 tgttgacata agaaggcatt aagatggcgc cgctttcaat aaagctgcct cttcgggcga
     7801 gggcgtgggg aacggagcgg acgccttcac tcccagtcca acgtttgacg gggattttat
     7861 cgaagtaatt aaaatccccg gcgttcatca agctcatttt ttgaaggcgg aagtaaagca
     7921 gaattccttt tttaagccac tcatgggtgg tccaagtgcc ttcgattttt tcacagactc
     7981 taagggagcc ggaatcaaga ttctgaatgc attgctcgat ggcggatttt ttttcgctgg
     8041 aaaggtcatc gattttagcg ccagcttgaa cttcgttcca gatatgttga attgaatttg
     8101 caaggctcat attatttcct tttctcggtt actttaaggg cctcaagaat ggcaggcaca
     8161 tggattttca aaatctccgc cgtgtcttgg ccacgttgga tttcataggt taacgtaggt
     8221 gagttgcgtt caaggcccgc ataggagcct aaacatcctg gcgtagggta accgatatcg
     8281 gcatcgattt tatagcctgt gagtcgagcc aaaacttcgg cttcgccatg acaatcgccg
     8341 ttcacgttta agatgggatg ccacgagtgc aaagataaaa tccattgcgg ctttttctca
     8401 tcaatgtatt ccataagcgc ttggttttca ggttcgctgc cggcgaaggg gcctggatgg
     8461 taccgtgggg ttttcacttc aggagaccag tcttttgttt ttagattccg attcagatca
     8521 atgcccttgg cattgccgcg agttttgtta accacacctt cgatgttgaa ttggggaact
     8581 ataaccaagt ttaggtgata ggaaaaatct ctcatgaagt ggtttaagag cgcctgtgcg
     8641 gcaaagactc cttcgacttc gtctccatgg actccgccca aaattaaaac ttccgggccg
     8701 gaattgcgga attcataggc tatgatgggc agtcccaaag agctatatcc aaaaacagat
     8761 gttttcatac gtgatccttc tcgctagact gaataaaaca tcatgaggat gagagatgtc
     8821 aaaaacgaag ttgtatttgc aatatgagaa tcagaaattg gtgctgggcc cccaaaaaaa
     8881 ggctttagat tgcctgacga aaaactatga acgaccggtt tatgtttacg atttggacat
     8941 tttaaaggag cgctttcagc ttttacaaaa agctttgcct ggggtccaag tgtattatgc
     9001 catgaaagcc aactcccacc ctgaagtttt aagatgtctt catgaactcg gcagcggggc
     9061 agatgtagtg agcggaggtg aaatccgctg cgccagcgaa gcgggattta aaccgcaaca
     9121 aatgatctat agcggtgtgg gtaaaagtca taaagagatt cgccagagct tgggattaaa
     9181 tttagcgcaa atcaatgtcg aaagtatcgc cgaacttcgt cgcattgcca gcatcgccaa
     9241 agagcttggt caaaaaccgg cggtggtgtt taggctcaat cccgatgtcg acattgagac
     9301 tcatccttat attgctacgg gactgcacaa taataagttt ggcatggccc tgagcgatct
     9361 tccggagttg aaggaagtcc tgtatgcgaa cgcttctttt ttagatttcc ggggagtgag
     9421 tttacacttg ggttcgcaaa tgcacaacct ttccggcttc cgagatgcgc tcaaactttt
     9481 gcgtcctgtc tatgaggatt tgcaaaaaga gttcagttcc gtggacactt tcgatgtcgg
     9541 cggaggttta ggaattttct acgaaactca agagttggcg gcggaagaaa agctcctggc
     9601 ggattatgcc accattattt ttgaagaact gaaaggcttg cgggcaaaaa ttcaaacaga
     9661 gccggggcga tggcttgtgg ctcatgctgg agttctacta acccagattc agtatctaaa
     9721 aaaaacttca acaaaaaatt tcttgatcgt agacacgggc atgcatcact tgctgcgtcc
     9781 cacgctttac ggggctcatc accgtgtatg gactttgaaa gaaagcccgg ggggaggcag
     9841 cgcaggtggc gtcatgaaaa aatacgatgt cgtgggacct atctgtgaat ctggggactt
     9901 cctcgccacg gaccgcgagt tgtgcgagat gaacgaagga gatttcttag tcatcggtga
     9961 cgtcggagct tacggttttg tcatgcgctc ggattataat ctgcaggatc cgccggatga
    10021 ggtggtgttg taggggggca atcttatttc cggtgttatt tttgtcaggt atttcgattg
    10081 ccttctaaag cggaaaatct ttgctgataa tagaaatcac aaaatgaggg acttctactt
    10141 catcaccatt atatccagta gttatatgta ttttagtgaa acccttatta aatgctctca
    10201 tcgctggaag caacggctta aaaagggacg attttcaacg ggataccggg gacatggtca
    10261 aataatggac atcggtttga aaagggacca cagagttcat ttctattctt ccgtttctta
    10321 tttacttgta tgacttttat tgccgtgtca gtccactgga gttagcggta acagtttaga
    10381 ttgcgttagg ttcttgaaga cggcgattct ttgttcgagc tcgaaattat ttcttagttt
    10441 tcacgatggt tacattcaaa actgtgtgaa aggatcttct ttagaaaatt cctttttcgc
    10501 cagaatacaa tccgtgaact ttgcatttcg gtgctgcaaa gggtgctagg ctcacttttt
    10561 tcatcaaccc aaccatcaac ctgcggccaa tgttcaactc atgaagaaag gaaaattagc
    10621 gcgtttacac aggtaactga aatttgaatc accttaagct ttcacgcctc atgctgcgag
    10681 ctcggccctc tttttttcaa atggcaaaag tcccttttga aagcggtgat tttcgaccac
    10741 ctggtaccgg taaaccggtg aaaactgtcc attttgaaac cggcgcctcc atataatggg
    10801 tgaattgtaa tgcttgcagc gattaaatta acacaaataa cttctttggt acgttcaatt
    10861 ctacgttatg attattcatt tgctaaattc ggaccaatct taaaataact ttattcgatc
    10921 tattaccaga taagtgttta aaattggatt ctttaagacg acgattgtcc cttataaaag
    10981 tttgccactc gtaaagtatt taagcagcgg cggcatcttt taattcataa tacaacttga
    11041 tccacttcac taagctaaca tcggagtcat ccattttgtg aactgcagaa tcaatcctct
    11101 cgaaattatg attgacggct acatagtcag gtcggatcgt tttccagtga cgatatatca
    11161 tgcaaactgc gtagatcgcg gagagctgaa aatttttgtt ttttgaggtg agccagtcca
    11221 ataatatttc agcatgttcg tcagtaaggc cgttgatttt aatcaggtca atcaacgagt
    11281 ccgcagcaat acgattgtcg tcac
//
LOCUS       DATBTE010000095        21158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_967254, whole genome shotgun sequence.
ACCESSION   DATBTE010000095 DATBTE010000000
VERSION     DATBTE010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 21158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21158
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967254"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1429
                     /locus_tag="VIG33_03785"
     CDS             110..1429
                     /locus_tag="VIG33_03785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8269982.1"
                     /translation="MSLINPTAQQKKSLERWRLSTFWVMLIGYIGYYIGRGNLPVALP
                     LLSKEFGYSNTDLGIILTLSELAYALGKFTTGPLADKVGGKLIFIIGLVGAIVFNLLF
                     PLFSSIFMFTVIWCFCRYFLSMGWGGIIKTIGEWYEPERNGTIMGIISINFQFGSVFA
                     SLFCSFLLMLGFGWKALFYYPAMVMILVVIWAYFSSKESPHDLIPNVRFGRYAGHKDS
                     LAHFKEAHGKRSAREILRGLLKIRMFRQILVFSFFTHLLRSIFLFWTPKFMVDMGMGQ
                     VAAAMTSAIFPLLGCLGTLFLGWYTDRHAKDGDRARMMWKMLVGLVISLVIVAALVPF
                     KLEYQYALVGFLGLAGFCLYGPYSMSSGCLTLDIAGSEAAGTCTGMIDGVGYIGGALA
                     AWGAGYLSDSLGWSQVFYVLAGFAVLSVLSAYVMSRAYQQDLKGEKV"
     gene            1426..2304
                     /locus_tag="VIG33_03790"
     CDS             1426..2304
                     /locus_tag="VIG33_03790"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY8269983.1"
                     /translation="MTTRQKWIGIYILAVSALAQARPLEVQGHRGARWVRPENTLPAF
                     EYALEAGVDTLELDMLVTKDGQVVVHHDPLLNPDICLDPQGKKITDKISIRSLTLKEL
                     KAYDCGSLVNPRFSQQRAQPKTSVPTLDEVFDLVEKSKFPVAKKVFFNIETKSEEDHP
                     EYSPAPEEFIKLVLAIVKKHKLMDRVTLESFDLRTLKVARKLEPKLPLSILIEYRPKE
                     AGALVKLMKENQAQILSPNYEWLTAQDVADIHKLGGRVIPWTPNTKKDWQSLIEKGVD
                     GLITDNPKELLETLKR"
     gene            complement(2411..2701)
                     /locus_tag="VIG33_03795"
     CDS             complement(2411..2701)
                     /locus_tag="VIG33_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269984.1"
                     /translation="MEQNLSTDSLAQDQTQREGRFTKALEKQTAKVPSGVYLGLAVGS
                     VLLSAGLAMSEKRKGWANFVGLWVPTILLLGVYNKIVKIEGSDKMEKSQILH"
     gene            2927..3457
                     /locus_tag="VIG33_03800"
     CDS             2927..3457
                     /locus_tag="VIG33_03800"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY8269985.1"
                     /translation="MKHVNAFFGTVASLVLMAGIASAAQGNLNDNQVIGLINKVNDVE
                     VSIGKVAVDKGQEDSVKKFAAKMVAEHTASNVKIAALETKTKLSRAESDVSKTLEKNA
                     NTMIESLKNAKSSEFDKAYIDNEVTMHQQVLNTIDQNLLPNVQNADLKSFLQSTRGEI
                     ASHLKEAQDIQSGLKK"
     gene            complement(3529..4080)
                     /locus_tag="VIG33_03805"
     CDS             complement(3529..4080)
                     /locus_tag="VIG33_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEY8269986.1"
                     /translation="MYRKRNGLKKSSDQFVRQCLGNFLDEDPSSLNISRHPSGQPLVE
                     YQVPVYFSLTHSGDFSSLACSLNFDLGLDLQVHQHFNPNLFSTLCTKSELASLKELNQ
                     ENFFKIWTIKEAALKCLGLGLRFPMNQLHINFDNNHIEVLSDQTKYNWLTYAELSLFE
                     NSTTHLVWNRNDRKPNMVLISEV"
     gene            4147..5772
                     /locus_tag="VIG33_03810"
     CDS             4147..5772
                     /locus_tag="VIG33_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8269987.1"
                     /translation="MKISCERAGNKNIISVLELDSLKRDYVIRSATGEDFYKQTQELS
                     NAQKISGINNSARVALIAEGSVNWALNFFACFLNGATLCPVDVKLTPAELTSLLEHFG
                     ATHALVSDLMWEKLVELKAVAEHRIKLIKFSDFKKFPSALVNSLIADTGTPAQDADLI
                     IYTSGTSSNPKGVVISLSSLFFEAESVNRTIYPADAAQRVVFSILPLNHIYGLSFGLL
                     CTFWSGNEFCMSQSLEPQNIQRMTKEQKVSCFMTIPLFLSLLKNGIETKVRQLSPVKR
                     TLFKLMMSVSLKLQSEFVGRWFFKEVREGVGRHIEFFICGGAVLDEVTLRFFEALGWP
                     IYNGYGLTETGPLISGNTPFKSKRGTVGQVLSGVEVKIQNGEICTKGPHVFKQYLNAE
                     DLTREAFTSDGWFRTGDLGSLDSEGFLSIHGRLKSMIVLSSGKKIQPEEVEDALMASK
                     YVKLACLVAIPSKGVGEKIVAALELHEPYSASISDQKFLDTVTEELKFLCQNLAPFKR
                     PQIYTFRQEPFQVTSSQKVKRHWVQKEIENSLQ"
     gene            5769..6758
                     /locus_tag="VIG33_03815"
     CDS             5769..6758
                     /locus_tag="VIG33_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269988.1"
                     /translation="MKIVLTLLTLVAFYVFTLLHDAGVFRRIKDVKYGQCQKIGPALG
                     PEDMVLTNTKAGVKVLVSSDARTTEAASGHLYEYDLQNRHLRDMTSELNLPFPFHPHG
                     MDLWTEGTGKTLVYVVNHMDELHTRIEVFEWGPEIVFVKSIENELFQSGNDLVAIGND
                     RFYLTSDFGTPNKFQQKLQSYLWQKTGYVTHYDGNTAKKIANNLFYANGITKGFENRI
                     FVSAMLEKAVHILEGEVIPLEGAPDNITWYQEKLYIAAHANLFALKKESEDRKNRAPS
                     KIIEWDGKKATTIYENAGDEISSASIALPIDENRLLIGSVFDNHILDCTRSKP"
     gene            complement(6755..12616)
                     /locus_tag="VIG33_03820"
     CDS             complement(6755..12616)
                     /locus_tag="VIG33_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8269989.1"
                     /translation="MKAFSTQEIAGIIAEEISAMTGLSIDEIPQSQGFLELGLESLQA
                     VSIVEKVGKRISQKIDVTAMFDYPSILSLSQFLSGEKDSSYNNSHRHMMDDLGATSAI
                     AIVGMSGRFPKAHNLEEFQHNLVNKVHGIEEIGVQRWGPTPDRAINFIGALSDYDQFP
                     ADAFGISELEAQRMDPQQRLVLEEAWFAIEDAGYSAEELRGSMTGVFVGISSSDYTHL
                     VQQEGQIDIFSATGNAHSIAANRVSYFFDFKGPSLAIDTACSSSLVAVHLAMDSLRRG
                     EIDLAIVAGVNLVLTPELSEAFSGATMLSPDGRCASFSKNANGYVRGEGVGVVLLKRQ
                     ADAIEDRIYALVKGSAINQDGKSNGLTAPNGKAQEAVIREALRRAQIEPQAITYHEAH
                     GTGTPLGDPVEMLALQRVHQGRTLPLLVGSVKTNIGHLEAAAGIAGFIKLALSLYHKK
                     IFPNLHFDELNLQLKDKIPALKVADRGSEWTASSAQPRCGSVSSFGFGGTNACLILSE
                     STEAVTQVHVSPVPQGIVAVAGFSSNSNEGALQSLEKFAQSIESLDWNTVCANAQATL
                     SQRMSLPHRVLISAESQEELLERIRSHKAKSFQVGVPSQRICFLFTGQGSQYTQMYFA
                     HYSSSNSFKKRVDDLLRDAQLFYPLNLFEVWTDRKYEKELQQTDYGQILLFIFQLVFA
                     EHIKKECNVEPEFVFGHSLGEIIAAVFSGILSESEGLILVAYRGRLMRNTAVGAMTAV
                     FATKVQLDPLMSSGLKLDYAAFNGPQLQVVSGAIHEIEKLETLLTEKAFDFRRMTAQQ
                     AFHSALMDPILDVFADSIHSFQFRSSNYKFISSATGELLRANDTLDAGYWKNQLRSPT
                     QFEKAMTTLKSSKATVFIELGPQPVLNGMAQRIIQDVNSTWIHFFESAEKAGSVYANG
                     LIRGLLQAVFHVKTKQRVFDLPKTVFSKKSFLISKKPTSKPVLSSSTKAANPAEILDQ
                     LVEIVASVMRVPKNEISVDENLVDLGADSLVLMNAIQSIKDNYHVAIPVSEVFKTLNT
                     MQKMADFVCSEIMQKQPEVMQSQNIKMPSASFTSLGKEKKGVLGNFRTFASLEKSQDE
                     ANERKQYLQTFIKDFNSKTPKTKAHVEKYRDVLADNRTSAGFRPETKEMIYPIHCYKA
                     KGSRFTDIDGNDFIDFTMGFGVNLFGHSPDFLEKAMLEQIQLGVCVGPQSFLVGEVAH
                     LVCELTSLERTAFVNSGTEAVMTAIRLARAGTKRTKVVLFDGSYHGHFDGVLARGTAD
                     LKSIPVASGIPPHMVDDIIVLEYGSEASLQIIKDLGNTLAAVLVEPVQSRFPEHQPQQ
                     FLKEIRRITEVQGTAFVWDEVITGFRIHPGGAQAHFGIKADIAAYGKILGGGMPIGAV
                     AGSKKYLDFIDGGHWQFGDDSLPDKEMTFFAGTFSKHPLAMATAFETLKLLREKGTQI
                     IGALNTKTAALCQELNQFFTEQDLDITMVYFGSLFRFKANLNLDLLVASLNKAGFYIW
                     EGRNLFMSTAHTDEDIQLFKKSIRELTLTLLRTGHFTKRKVQAQKTYPMIAPQKRFRE
                     LSAVSSLGASASHICISAKVKGFLDPLKLQQALNILARKKDLFRWRADLSQNSQFFEK
                     APADIDFEAINLRQEKTPWKALDKMLFELSLKNLDIEKSSPILVKLYEVVEETHVMAV
                     FCHHLIFDGWTMTLFFEDLATIYNALLGDKIIDLPPSLNFEEFLGSENVWAKPQDLKQ
                     AELFWLNEFKGFQSKNSFAALKGISTEEYRGQRLVFDLPLKLYKELKVFSKAQRITPF
                     MYLLGAFAKTLMIKLDKDELVIGIPAANRDFPGSDKMYGNCTNLLPIRLKDSSADPVE
                     FYHSVKEKVIQAYQHMAYPYEQTKEKTGPLFDIYFNLEPTSDLPEFDQASLIIHPFPI
                     SASEFPLMLNITDFEHYYHCEIDFQWKAISDDFVLELIDDIKKLIQDSLKATP"
     gene            complement(12626..13516)
                     /locus_tag="VIG33_03825"
     CDS             complement(12626..13516)
                     /locus_tag="VIG33_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269990.1"
                     /translation="MSTAVSVKSLLKKVNSQELDHPRKVGRDMIESTLPADLWKANVE
                     FSHELQKSIDKHKLCRHPLIGLLNTEKLNVEATKVIHLEFSYAFAEIFTDSLIQAMST
                     CSSLEPRLGAAGKVSARFLLQLNLLDELGYKPKSNAANSEDYCGNPFLAHYIQFNETL
                     EDLGAKHSDIMNFKPSAAAKAARKTFTDYYNDHLNLTAVLAVAETVFSLFAGPWAKSV
                     SLSTDIDVSKGYHKIHVENESGEFLDDDHSEDSWYIFRQVVQAKDYDLIRENISQWLD
                     IWYDFGDQVIHIANQLSKKK"
     gene            13758..14177
                     /locus_tag="VIG33_03830"
     CDS             13758..14177
                     /locus_tag="VIG33_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269991.1"
                     /translation="MTNYGNLNIHLGWMTMVMGILTGSILGMWSFAGPFKTPKGHHNY
                     GDLPRRMNRLAHIALFALPLISIVLGQYIDAVSMPDNIKLAASYCWIICMWGVPLFLF
                     MASLYNPLKYLEIIPVSCGTFSLFVMAYDHFLKFTGS"
     gene            14179..15561
                     /locus_tag="VIG33_03835"
     CDS             14179..15561
                     /locus_tag="VIG33_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY8269992.1"
                     /translation="MIPPKTNRLKKLWSLAWNNVRLYRRRHFFTGLGLSAGFCALILL
                     GGYMLRMEEYLAVHSIFLRQVGHLSVWKKDSLKLYFQNPEKYSFSPEEQQKIKRALAP
                     ILKAEDRVIPVLSGTGMITNGCQNLPIIFAGIDPQDSEWIYSHPQVHQQIPELVHFDQ
                     GVGYWEPEGSLMPINLSPILFSLLKKKNLFSGGTLSAGEVVNCSHPDSLIKNSADPSV
                     QIFSAAFDGGIGITDALLVGLKSTGFTFSDETAVMLSRDVAKNLLRTDNVSRMVIMLA
                     NPENSKSFRVAVAGAIEKAGVDNFEVLHYTDESVSPIYVGAMNFVYVMMGFFVVLVCG
                     VVILSIANSFQIAFMERRHEFATLRSVGFRSGVLRTLLMFEYIQMAAISFAVGGCLAF
                     ILKGLNNALNLRFQIPGFANDLQFKLEPTFIFTYSVALAMMIIVVVSVHFFARQQMKL
                     KVIELMGTEA"
     gene            15558..16853
                     /locus_tag="VIG33_03840"
     CDS             15558..16853
                     /locus_tag="VIG33_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY8269993.1"
                     /translation="MILFKIIQLAFKNIMNSRKKSFSAILSVASGFLALNLFQAYIVD
                     TNILFDQTYENRSMLGHLLIRKESRLDLTTAEEQKEIIRFLTENGNEIEVVVRFLNFT
                     GTLNSGSVQTVFSGYGYDIDQGRKIRGASWQWNVVAGEPLDQAPNTRGLLLGRRLAET
                     VKCKENQPLDVLISKAGYEAKTRGFQCENPSLQLSISTQTGQVNAAELKVLGILDGVF
                     RDIDERLVVTSLAEAQSLMHSDEISMIALRLKEVGKGDELKSRFSQTVQKKHPQLQLK
                     SWKEYEIGDFYNKSINFLFIFRNFITLVVISIGALSVMTTFYRLVFERTREIGTLLSV
                     GFKSKHIFTLIISEAFFLSLLGIAIGVLASYIVAALVNSLEIPYKLGMLSEAVPFAMT
                     IQFKMLFLSALTLIFISIGICAFPVKRALKMKVSDALRE"
     gene            complement(16843..17517)
                     /locus_tag="VIG33_03845"
     CDS             complement(16843..17517)
                     /locus_tag="VIG33_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8269994.1"
                     /translation="MIQIENLYKTYQLGETKVPALRGLNFRVQKGDFRALLGASGSGK
                     STLLHLLATLDTPDQGRLLFMGQDLATLNEDQKSDLRNSKISMIFQNFNLIPALNVKQ
                     NIELPLILRSDLSDEEKEQRIRIAINDVGLNEHMNHKPDQLSGGQRQRVAIARALVPQ
                     PELILADEPTANLDSATAHQVIDLLLDLNQKRNITFLFATHDEKLIQRVKNLSWIVDG
                     VIKTTP"
     gene            complement(17530..18750)
                     /locus_tag="VIG33_03850"
     CDS             complement(17530..18750)
                     /locus_tag="VIG33_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269995.1"
                     /translation="MFNFDFRSDFLTGGFLQHNNEDITNPNNQLLYLPSQAFDVEIKP
                     TLKIDSTHYNLKVVLIPRLQFHSARYNFGSVNESQRLADYGFWEAYTHWELIKNVNLS
                     VGIINSQWGPSDILSPSQFLFSDLLIKLEPFQSTQGVEMAVVQWTPNQNFTLTGMGEL
                     DPFGWEHTDTPPYRDRTSKKRVLLRGEFATGSGDFNLGLVLGRKHTDRERAQAGSYAM
                     WNYTNFTQIYYDIGLQQGSERLYIDDTGTLLPAYADDSTAFQVSLVGHRFTFVNGIEW
                     RIEYLHNDFGPSKEQRQRTYRLIHATPLATQYLSILNDSVSPLPGQEFIYNSFRWDNP
                     PFLEAVFSNSHIYLRNIFSLTDPSQLFNFAIETSLTDQLSHSIAFIATSGDNESELSS
                     YMHSYASYFLKFSF"
     gene            complement(18825..19562)
                     /locus_tag="VIG33_03855"
     CDS             complement(18825..19562)
                     /locus_tag="VIG33_03855"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEY8269996.1"
                     /translation="MKTLLFATLFFLSFQTYAVKTPDAVELLKAADRARGGLSGGLSW
                     KLQLVSKDGAKENSFEYDVKVKASNVLAKCIAPARSKDEVYLFLDRNLWIHRPGLRKP
                     LSLSSRQRLSGQAANGDIATTNYARDYTPTLSGEEAVDGVKAWKLKLKAKSNDLTYDQ
                     IVYWISQDKLLGIQAEFLTLDGDVFKKAKFSYDNSVSYNGKTLPFVSKTIIVDAKNSE
                     LKSELIYSKPNASSLNDADFNVSNLTK"
     gene            19705..21003
                     /locus_tag="VIG33_03860"
     CDS             19705..21003
                     /locus_tag="VIG33_03860"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8269997.1"
                     /translation="MRIRHPFAKENQKIVIIVGGGFAGLAAANMLAEEPEVYVILIDK
                     RNHHLFQPLLYQVATGGLNASDIAVPIRSQFSKVSNVEVHMAQVEAVNVQESFITTER
                     NEILFDFLILACGAHHSYFSNTEWAAFAPGLKTLEDAIDIRGRIFRAFERAENELDLE
                     KQRALLNFAIVGGGPTGVELAGAIADVARTVLIKDFKRINPASARILLLEAGPKILSA
                     FSEDLSRHARNDLEKLGVEVRTGAKVEAITDYGLIIGGEFINTYNVFWAAGVQAEKMN
                     ITPSVAMDAVNRIKVEKDFSIPGFPYCFVVGDMATMQLENNRPLPGLAPVAMQAGKHA
                     AKMIKSSLQGLAREPFHYVDKGLMATIGKRKAISQYKSLKMTGYIAWLAWLFIHVYYL
                     VGFKNRVFVIIQWAWSYVFSKRGARLITGKESPSASSTIS"
BASE COUNT         5736 a         4259 c         4997 g         6166 t
ORIGIN      
        1 aacgaaatcc ctgtgaaatc attaaagtgc cctagctgtc gggatagttg tccggtcttt
       61 gattgacccc cgactagggg ctgttattct tttatctcga ggtaaaagaa tgagtttaat
      121 taatccaaca gcgcagcaga aaaagtcatt ggaacggtgg cgtctttcaa ccttttgggt
      181 gatgttaatt ggttacattg ggtattatat cggtagaggg aatttgcctg tggccctgcc
      241 tcttcttagt aaagaatttg ggtattcaaa tacagacctg ggcataatcc tgacgttgtc
      301 tgaactcgct tatgcattgg gaaaattcac cacaggccct ttagctgata aagtcggcgg
      361 aaaattgatt tttataattg gattggtggg ggccatagtc tttaatcttt tatttccact
      421 tttctccagc atttttatgt ttacagtgat ttggtgtttt tgcagatatt ttctttcgat
      481 gggctggggg ggaatcatca agaccattgg tgagtggtat gagcccgagc gtaatggcac
      541 catcatgggt atcatcagca tcaatttcca attcggcagt gtctttgcgt cacttttttg
      601 cagctttctt ttaatgctgg gtttcggttg gaaagcactc ttctattacc cggcgatggt
      661 catgatctta gtagtcatct gggcttattt tagttccaag gaaagtccgc atgatttgat
      721 tcctaatgtt cgatttggtc gttacgctgg acataaagat tctttggccc atttcaaaga
      781 agcccatgga aagcgtagcg ctcgtgagat cctcagaggg cttttgaaaa tccgcatgtt
      841 tcggcagatt ttggttttca gttttttcac tcatcttttg cgcagtattt ttcttttttg
      901 gacccccaag tttatggtcg atatggggat gggacaagtg gccgccgcaa tgacttctgc
      961 tatttttcct ttgttaggat gccttggcac tctttttttg ggatggtaca cagatcgtca
     1021 tgccaaagat ggagatcggg cgaggatgat gtggaagatg cttgtaggtt tggtgatcag
     1081 tctggtcatt gtggccgcac tagtcccctt taaacttgag tatcaatatg ctttggtggg
     1141 atttttgggg ctggcgggat tttgtcttta tggtccttac tcgatgtctt caggttgttt
     1201 gactttagac atagcgggct cggaagccgc gggaacgtgt acgggaatga ttgacggtgt
     1261 cggctacatt ggtggagctc tggcagcgtg gggagcggga tatctttccg attctttagg
     1321 ttggagtcag gtattttatg tccttgcagg ttttgctgtg ctctcagttc tttcagctta
     1381 tgtcatgagc cgcgcttacc agcaagattt aaaaggagaa aaagtatgac tactcgacaa
     1441 aagtggatag gaatatatat tttggcagtt tcagcattgg cacaggcaag gccgctggaa
     1501 gtgcaagggc atcggggagc gcgctgggta cgaccagaga atactcttcc agcgttcgag
     1561 tatgcacttg aagcgggagt ggacaccttg gaattagata tgcttgtaac caaggatggg
     1621 caggtggtgg ttcaccatga ccctctctta aaccctgata tatgtttgga tcctcaagga
     1681 aaaaaaataa ccgataaaat ttccattcgc tcactgactt tgaaagaatt aaaagcttac
     1741 gattgtggtt ctttggtgaa tccacgtttt tctcagcaaa gagctcaacc aaagacttcc
     1801 gttccaaccc tggatgaggt ttttgattta gtggaaaaat caaaatttcc tgttgctaaa
     1861 aaagttttct ttaatattga aacgaaaagt gaagaggatc atcctgaata ttcccctgcg
     1921 cctgaggagt ttataaagct tgttttggcg atcgtgaaaa aacataagct tatggatcgg
     1981 gtgactttag aatcttttga cctccgcact ctgaaggtgg cccggaagtt agagcctaag
     2041 cttcctcttt caattttaat tgaatatcga ccaaaagaag caggggcctt ggtgaagcta
     2101 atgaaagaaa atcaggcgca gattctttcg cccaattatg agtggctaac ggcccaagat
     2161 gtggccgata ttcacaagtt gggtggccgg gtgattccct ggactccaaa tacaaaaaaa
     2221 gactggcaat cgctgattga aaaaggagtg gatggtctta tcacggacaa tcctaaagaa
     2281 ctccttgaaa cccttaagcg gtaaattgtt cagacagcac tcccacaatg atcaagtgtc
     2341 tgactttcga ggcactttta tgactgtcgt cgggcgtgct gtttatatat tttttatttt
     2401 ctgaacagac ttaatgaagg atttgacttt tttccatttt atcagagccc tcaattttca
     2461 cgattttgtt atagacaccc aaaagtagaa tagtaggaac ccaaagccct acaaagttag
     2521 cccaaccttt tctcttttca ctcattgcca gccctgcgga aagaagaact gagccgacgg
     2581 ccaatcctaa gtagactcct gagggaactt ttgcagtttg cttttcaagg gctttagtaa
     2641 agcgtccctc tctttgggtt tgatcttgcg ctaagctatc tgttgataaa ttttgttcca
     2701 taatttgctc ctttgttttt gcacaccaag tgtgtagtta atttacaaac atctacgaaa
     2761 ctataagttt aaggcgggca cattgaaaat ggcgtgatat tttcgacatg tttgcaaagt
     2821 acaaaatgtt ttatgaaacg aatgctgttt tggaaagttt cgtcacttga ttttgagtca
     2881 aaagagcaag aatcttcacc cacaaacttt aaaaaaggag ttttctatga aacacgtaaa
     2941 tgcttttttc ggtactgtcg cttccttggt tcttatggca ggaattgctt ctgccgctca
     3001 agggaacttg aatgataatc aggttattgg gctgatcaat aaggtgaacg atgtagaggt
     3061 ttcaatcgga aaagttgccg tcgataaggg gcaggaggac agcgtaaaaa aattcgcggc
     3121 caagatggta gctgagcata cggccagcaa tgtaaaaatt gccgcgctgg agacaaagac
     3181 caaattgagt cgcgctgaga gcgatgtgag taaaacttta gagaaaaatg ccaatactat
     3241 gattgaaagt cttaagaatg cgaaatcttc tgaattcgat aaggcctaca tcgataatga
     3301 agtaacaatg caccaacagg tattgaacac cattgaccag aacttacttc ctaacgttca
     3361 gaatgctgat ttgaaatcct tccttcagtc aactcgaggc gagattgctt ctcatcttaa
     3421 agaggctcaa gatattcagt ctggtttgaa aaaataaatt agaattgttc agcgaattag
     3481 aagttcactt tgaggccttt tatataaagg cctcaatttt ttacacattc atacctcaga
     3541 tatgagaacc atatttggct ttctgtcatt tcgattccaa accaaatgtg tggttgagtt
     3601 ttcaaagagt gaaagttcag cataggtaag ccagttatat tttgtctggt cactcaagac
     3661 ctcaatatga ttattatcga aatttatgtg cagttgattc atcggaaaac gaagtcccag
     3721 gcccaggcac tttaatgcag cctctttaat ggtccaaatc ttgaaaaaat tttcttggtt
     3781 aagttctttc agagaagcta gctcggattt ggtgcaaagg gtggaaaaaa ggtttggatt
     3841 aaagtgctga tgcacttgca gatccaaacc tagatcaaag tttaaagagc aggctaggct
     3901 cgaaaaatca cccgagtggg tcaggctaaa atagacgggg acttggtatt ccaccaaggg
     3961 ttgtccactg ggatgacgag atatattcag tgagcttgga tcttcgtcaa ggaagtttcc
     4021 taagcactgc cgcacaaatt ggtccgatga ctttttaaga ccattccgtt ttctgtacac
     4081 tcgtatcatt ttccgactat acttgaactt gtggaaaatc accaagccaa aacaataaga
     4141 gattttttga agatttcctg tgaacgtgcg ggcaataaaa atataatttc tgttttagag
     4201 ttagattctt taaaacgaga ttatgttatc cgctccgcga caggggagga cttttataaa
     4261 caaacccaag agttgagtaa cgctcaaaaa atatccggaa ttaataactc ggctcgggtc
     4321 gcccttattg ctgagggctc tgtgaattgg gcattgaatt tcttcgcctg ttttttaaat
     4381 ggggcgactc tttgtcctgt tgatgtgaag ttgactccgg ctgaactcac ctctttgcta
     4441 gaacactttg gagcgaccca tgctttagtc agcgatttga tgtgggaaaa actggtggag
     4501 ctcaaggcag tcgcagaaca ccggattaaa cttattaaat tttctgattt caagaagttc
     4561 ccttcggctt tagtaaatag tctgatcgcg gatactggga ccccagctca agacgcggat
     4621 ctgattattt atacttctgg cacttcgtcg aatccaaaag gagtggttat aagtctttct
     4681 tctcttttct tcgaagcaga aagcgtcaat cgaactattt atccagcgga cgctgctcaa
     4741 agagtggtct tttcaattct gcctttgaac catatttacg gactttcgtt tggtttacta
     4801 tgcacgtttt ggagtggcaa tgagttctgc atgtcacaga gccttgaacc gcaaaatata
     4861 caacggatga ccaaagaaca aaaagtcagt tgctttatga ccattccgct tttcttatct
     4921 ttgctgaaaa acggcattga aaccaaggtt cggcaactaa gcccggtgaa aagaactctc
     4981 ttcaaactta tgatgtccgt gagtttaaaa cttcaatccg aatttgtcgg tagatggttt
     5041 tttaaagagg ttcgagaggg tgtaggaagg catatcgaat tctttatttg cggtggggcc
     5101 gtcctggacg aggtgactct cagatttttc gaggcgctcg gctggccgat ttataatgga
     5161 tatgggttaa cggaaactgg acctttgatc agcggaaata ctccctttaa aagcaaaaga
     5221 ggaacggtgg ggcaagtgct ttccggagtt gaagttaaaa ttcaaaacgg agagatttgc
     5281 actaaagggc ctcatgtttt taaacagtat ttaaatgctg aggatctgac tcgcgaagcc
     5341 ttcacgagtg atggatggtt taggacgggg gatttaggca gtttggattc tgagggtttt
     5401 ttatctattc atggacgcct gaaaagcatg atcgttttat ccagcggaaa aaagattcaa
     5461 cccgaagagg tcgaagatgc gttgatggct tcaaaatatg tgaaattagc ctgtttggtg
     5521 gcgataccaa gcaagggagt cggcgaaaaa attgtggccg cattagagct gcatgagcct
     5581 tacagcgctt ccatttctga ccaaaagttt ttggatacgg ttacggaaga acttaaattt
     5641 ttgtgtcaaa accttgcccc gttcaagcga ccgcagatct atacgttccg gcaggaacct
     5701 tttcaggtga cttcgagtca aaaagtcaaa aggcattggg tgcaaaaaga gatcgagaat
     5761 agcctccaat gaaaatagtt ttaactttac ttacgctcgt tgcgttttat gtctttacct
     5821 tgttgcacga tgccggcgtc ttccggcgaa ttaaagatgt caaatacggc caatgtcaga
     5881 agatcggacc tgctttaggc cctgaagata tggttctcac aaatacaaag gccggcgtaa
     5941 aggttcttgt cagttctgac gcgaggacca cagaggcagc ctctgggcac ttatatgaat
     6001 atgatctgca aaatcgacac ctgagggata tgacctcaga gttgaatttg ccatttccct
     6061 ttcatcccca tggaatggac ctctggacag aggggacggg aaaaaccttg gtctatgtgg
     6121 tgaatcacat ggatgaactt cacacaagaa ttgaggtttt tgaatgggga ccagagattg
     6181 tgtttgtgaa gtccattgaa aatgaacttt ttcaatcggg caacgatctg gtagccatcg
     6241 gcaacgatcg gttttatctt accagcgatt ttggtacccc taacaaattt caacagaagc
     6301 tgcagtccta cctttggcag aaaactggtt acgttactca ttacgatgga aacaccgcta
     6361 agaagatagc caacaactta ttttatgcca atggtataac caaggggttt gaaaacagaa
     6421 tatttgtctc cgccatgttg gaaaaagcgg tgcatatctt ggaaggcgaa gtgatcccgt
     6481 tggaaggggc tcctgacaat atcacctggt atcaggaaaa gctctatatt gcggctcatg
     6541 ctaatctttt tgccttaaaa aaagaaagtg aagatagaaa aaatagggct ccttcaaaaa
     6601 ttatcgaatg ggacggtaag aaggccacaa ctatctacga aaatgccggc gatgagattt
     6661 cgagcgccag catcgccttg ccgatcgacg aaaaccggct tttgattggc agcgtttttg
     6721 ataatcatat tttggactgc actcgatcga agccctaagg tgtggcctta aggctgtctt
     6781 gaataagctt tttaatgtca tcaatcagtt ctaaaacaaa atcatcgctg attgccttcc
     6841 attggaaatc aatctcgcag tgataatagt gttcaaaatc cgtgatattg agcatcaacg
     6901 gaaactcgct ggcggaaatg ggaaacggat gaatgatcag actggcttga tcaaactcag
     6961 gtaagtcgga ggttggttca agattaaaat aaatatcgaa aagaggtccc gttttttctt
     7021 tcgtttgctc ataaggataa gccatgtgtt gataggcttg aatcactttt tctttcacgg
     7081 agtgatagaa ctccaccgga tcagcggaag aatcttttag gcgaattgga agcaagttgg
     7141 tgcagttccc atacatcttg tctgatcctg gaaagtctcg attggcagcc ggtatcccaa
     7201 tgacaagttc gtctttatcc aactttatca taagggtctt ggcaaaggcg ccgagtaaat
     7261 acataaaagg cgtgattcgc tgggctttcg agaagacttt gagttctttg tataatttca
     7321 gtggcagatc aaagaccaga cgctggccgc gatactcttc agtgctgatg cctttcaacg
     7381 cggcaaaaga atttttcgac tggaaacctt taaattcatt gagccagaag agctcggcct
     7441 gttttagatc ttggggtttg gcccacacat tttcagaacc taagaattct tcaaagttga
     7501 gtgaaggcgg taaatcgatg attttgtcgc ccagcagagc attgtaaatc gtggctaaat
     7561 cttcaaaaaa caaagtcatc gtccaaccat caaaaatcaa atgatggcaa aacacagcca
     7621 tgacgtgggt ttcttcgacg acttcataaa gtttaactag aatcgggctt gatttctcaa
     7681 tatccaaatt cttcaaagaa agttcgaaaa gcattttatc gagagccttc caaggcgtct
     7741 tttcttgtct taagttaatg gcctcaaaat caatgtccgc aggtgccttc tcaaagaact
     7801 gactattttg tgaaaggtct gcacgccatc tgaagaggtc tttttttctg gctaagatat
     7861 tgagtgcttg ttggagtttt aaagggtcta aaaatccttt tactttggcg ctgatacaaa
     7921 tatgactggc ggaagctcct aatgaagaga ccgcactgag ttcccggaag cgtttttgcg
     7981 gagcgatcat gggataggtt ttctgggctt gaacttttct ttttgtaaag tgtccagttc
     8041 tcagtaaagt cagagtgagt tcacgaatgg attttttaaa tagttggata tcctcatcgg
     8101 tgtgagcggt ggacatgaaa aggttgcgac cttcccagat gtaaaatcca gctttattaa
     8161 gagaagccac gagtagatcc agattcagat tcgccttaaa tcgaaacagc gatccaaagt
     8221 acaccatggt gatatctaag tcctgttctg tgaagaactg attgagctct tggcagagag
     8281 cggcggtttt cgtatttaga gccccaataa tttgcgtgcc cttttctctg aggagcttga
     8341 gagtttcaaa agctgtggcc atggccaagg gatgcttact gaaagttccg gcaaaaaaag
     8401 tcatttcttt atcggggaga gagtcgtctc cgaattgcca atgaccgccg tcgataaaat
     8461 cgagatattt tttactgcca gcgacagcgc cgatgggcat tccgccccca agaatttttc
     8521 cgtaagcggc gatatccgcc ttaatgccaa agtgagcttg ggctccacct ggatggattc
     8581 taaagccagt gatcacttca tcccagacaa aggcggtgcc ctggacttca gtgatgcggc
     8641 gaatttcttt gagaaattgc tgtggctgat gttctggaaa acgactttgc acgggctcga
     8701 ctaagaccgc cgctaaagta ttgcccaaat ctttaataat ttgtagggaa gcctcactgc
     8761 cgtactcaag cacaataata tcatcgacca tgtggggagg aattcctgaa gcaaccggaa
     8821 ttgattttaa atccgccgtg cctcgagcta agacgccatc aaaatggccg tgataggagc
     8881 cgtcaaacaa aaccactttc gtacgtttgg ttcctgctcg cgccaagcga atcgcggtca
     8941 taacggcttc agtcccggaa ttgacgaagg cggttctttc caggctagtc aattcgcaaa
     9001 caagatgagc cacttcgccc actaaaaagg actgcggtcc aacgcaaacc cccagttgaa
     9061 tttgctctag catggctttt tcaagaaagt ccggagagtg tccaaagaga ttcactccaa
     9121 agcccatggt gaagtcgata aagtcattgc catcaatatc cgtgaagcgg ctgcctttcg
     9181 ccttatagca gtggatggga taaatcatct ccttggtttc tggtctgaat ccagcactgg
     9241 ttctattgtc ggccaaaaca tcacggtatt tttcgacatg ggccttggtt tttggggttt
     9301 tgctattgaa atcctttatg aaggtttgca ggtattgttt tctctcattg gcttcgtctt
     9361 ggcttttctc aaggctggca aaagttctga aatttccgag aacgcccttt ttctcttttc
     9421 caagagaggt aaaagaggcc gaaggcattt ttatattctg actttgcatc acttctggtt
     9481 gtttttgcat tatttcggag cagacgaaat cagccatctt ttgcatagtg tttaaagttt
     9541 tgaaaacttc agatacagga atcgccacat gatagttgtc tttgatggac tggatggcat
     9601 tcatcaaaac caaagagtcc gctccaaggt ccaccagatt ttcatcaaca gagatctcat
     9661 ttttagggac acgcatgact gaagcgacaa tttccaccaa ttgatccaga atctctgcgg
     9721 ggttggcagc ctttgtcgac gaactcagta ccggcttaga agtcggtttt ttcgaaatga
     9781 ggaaagactt cttgctaaaa accgtcttcg gtagatcaaa aacgcgctgt tttgttttta
     9841 catgaaagac agcttgcaaa aggccacgga tcagtccatt ggcataaact gatccggctt
     9901 tctctgcact ttcaaaaaag tgaatccagg tggagtttac atcctgaata attctttggg
     9961 ccatgccatt taaaacaggt tggggaccaa gctctatgaa gaccgtggcc tttgaagatt
    10021 tgagggtcgt catggctttt tcaaattgcg ttggactgcg taattggttt ttccagtagc
    10081 cagcgtcgag agtatcatta gctcttagta gctctccggt ggctgaagaa ataaatttgt
    10141 aattgctgga acgaaactga aaggaatgaa tggagtccgc aaaaacatct aagataggat
    10201 ccatcaacgc agagtgaaaa gcttgctgag ccgtcatacg tcgaaagtca aaggcctttt
    10261 cagttaacag agtctctagt ttttcaatct cgtgaatagc cccggacaca acctgaagct
    10321 gtggaccatt aaaggcagcg tagtccaact taagtcctga gctcataagt gggtctaatt
    10381 gaacttttgt cgcgaacacc gcggtcatgg caccgacggc tgtgttgcgc atcaggcgac
    10441 cacgataagc cactaaaatt aatccttcag actctgaaag aataccggaa aaaacggcgg
    10501 caatgatttc accgagactg tggccaaaga caaattccgg ttctacgttg cattcttttt
    10561 ttatatgttc agcgaaaacc agttgaaaaa taaaaagtaa aatttgacca tagtcggtct
    10621 gttgtaattc tttttcatat ttccgatcag tccacacttc aaaaagattc aggggataaa
    10681 agagctgagc atctcttaaa agatcgtcga ctcttttttt gaaggagttc gagcttgaat
    10741 aatgagcgaa gtacatctgg gtgtattgcg aaccttggcc agtgaaaagg aagcagattc
    10801 gttgcgaggg tacgccaact tgaaaagatt ttgccttatg cgaacggatt ctttccaaga
    10861 gttcctcttg ggattctgcg gagatcaaca ctcggtgcgg caagctcatt ctttgcgata
    10921 aagtggcttg agcattggcg caaacggtat tccaatccaa gctttcgatg gattgagcga
    10981 atttttctaa agattgcaag gcaccttcat ttgaattaga ggaaaatccc gcaacggcga
    11041 ctatcccttg gggaacggga gaaacatgca cttgagtcac ggcttcagta ctctcggaaa
    11101 ggatcagaca ggcgtttgta ccgccaaaac caaaggagct aacacttccg catcgaggtt
    11161 gggcagagga cgcagtccac tcgctccctc ggtctgctac tttcagcgcc ggaattttat
    11221 cttttagttg caagttcaat tcatcaaaat gtaaattggg gaatattttt ttatggtata
    11281 ggcttaatgc caattttata aaacccgcaa ttccagcggc ggcctcaaga tgtccaatgt
    11341 tggtttttac acttccgact aataaaggaa gcgtacggcc ttgatgcact cgttgaagtg
    11401 ccagcatttc tacgggatca cccagggggg ttccggtgcc atgggcctca tggtaagtga
    11461 tagcttgagg ttctatttgc gctcgacgga gggcctcgcg aatcaccgct tcttgggctt
    11521 tgccgttcgg cgcggtaagt ccgttggatt ttccatcttg attaatagcg gagcctttca
    11581 ctaaagcata gatacgatct tcgatggcat cggcttgtct cttaagaaga accacgccta
    11641 cgccttcgcc tcgcacgtat ccattggcat tttttgagaa ggaggcacag cgcccatccg
    11701 gtgaaagcat tgtcgcgcct gaaaaggcct cgctgagttc tggtgtgaga actaaattga
    11761 ctccggcgac aatggcgaga tcaatttcgc cacggcggag tgaatccatg gccaaatgaa
    11821 cagccactaa tgaagaggag caggcggtgt cgatagccaa gctaggtcct ttaaaatcaa
    11881 agaagtaaga gacccggttg gcggcaatcg agtgcgcgtt gccggtggcg ctaaagatat
    11941 cgatttgccc ttcttgttga acgaggtgtg tgtaatcaga actgctaatg ccgacaaaaa
    12001 ccccggtcat tgaaccgcgc agttcttcgg ctgagtaacc agcatcttca atggcaaacc
    12061 aggcttcttc taaaaccagg cgttgttgcg gatccattct ttgagcttcc agttctgaaa
    12121 tgccaaaagc atccgcagga aattgatcat aatcacttaa agcaccgata aaattgatgg
    12181 ctcggtcggg ggttgggccc cagcgctgaa caccgatttc ttcgatgcca tgaactttgt
    12241 taacgagatt gtgctgaaac tcttcaaggt tgtgagcttt cggaaatcgg ccgctcatgc
    12301 ctacgatggc aatggcggac gtggctccta agtcgtccat catatgacga tgggaattgt
    12361 tgtaggagga atctttttcg ccgctgagaa attgcgacaa agataaaatt gacgggtagt
    12421 caaacattgc tgtgacatct attttttgtg agattctttt accgactttt tcaacaatgg
    12481 agacggcttg gagtgattcc aaacccagtt ctaaaaagcc ttggctctgg ggtatttcat
    12541 caatactcaa accagtcatg gcggaaattt cttcggcgat gatcccagct atttcttggg
    12601 tgctgaaggc cttcataggg atcctttatt ttttcttaga gagctgattg gcgatatgaa
    12661 taacttggtc gccgaaatca taccaaatgt cgagccattg tgaaatgttt tcccgaatga
    12721 ggtcgtagtc cttggcttga accacttggc ggaagatata ccaagagtct tcagagtgat
    12781 catcatccag gaattctccg gactcatttt ccacgtggat tttatggtag cctttgctga
    12841 cgtcgatatc agtggataaa ctcacacttt ttgcccacgg tcccgcgaaa agactgaaga
    12901 cagtttcagc cacggccaat accgcggtta aattcaagtg gtcattgtaa tagtcagtga
    12961 aggttttgcg tgcggccttt gccgcagcag aaggcttgaa attcatgata tcagaatgtt
    13021 tagctcccag atcttcgagc gtttcattaa attggatgta gtgagctaaa aagggattgc
    13081 cgcaatagtc ttcactgtta gcggcattgc tttttggttt gtatcccaat tcatcaagca
    13141 gattaagttg cagcaaaaat cgcgctgaga ccttaccggc agcgcccagt cgtggctcca
    13201 gcgacgaaca ggtggacatt gcttggatca aggaatcagt gaaaatttct gcgaaagcat
    13261 aggagaattc caaatggatg acttttgtgg cttcaacatt gagtttctcg gtattcaaga
    13321 gtccgatcaa aggatgacgg cacaatttgt gtttatcgat ggacttttgc agttcatggc
    13381 tgaactcgac gttggctttc cataagtcgg cgggaagagt ggattcaatc atatcacgac
    13441 ccactttgcg ggggtggtcg agttcttgag agttcacttt ctttaataaa ctttttactg
    13501 agacggcagt agacatggct tctccattaa ttgtgttttg gtccataagg tttatcgtaa
    13561 tttgagaggt gtcccttaac tggaccttgg tagaggtcta caatattcgg ttcttaggta
    13621 aaacagaaga tatcccaggg aataatttgt ttaaaagtgc tctgataact taggtatagg
    13681 ttttgaacgg agagagcata gaaagcttag gaattgcctt gtgctatcat tttacaatac
    13741 agtgattcag aggtgttatg actaactacg gaaatttaaa tattcatttg ggttggatga
    13801 ccatggtcat gggaattctt acggggtcta ttttaggaat gtggtctttt gccggaccat
    13861 ttaaaactcc taaaggccat cacaactatg gggatttacc ccgacggatg aatcggctgg
    13921 cgcatatcgc cctgtttgcg ttgcctctta tttcaattgt cctcggtcaa tacattgatg
    13981 ccgtttcaat gcccgataat attaaacttg cggccagtta ctgttggatt atatgtatgt
    14041 ggggtgtgcc cttgtttctt tttatggctt ctctttataa tcctttgaaa tatttagaaa
    14101 tcattcctgt cagttgtggt actttttctt tattcgttat ggcttatgat cattttttaa
    14161 aattcactgg cagctaaatt gattccccct aaaaccaata gattgaaaaa attatggtcc
    14221 ctggcttgga acaatgtccg cctttatcgt cggcggcatt tttttacggg cttgggattg
    14281 agcgctggtt tttgtgcttt gattttattg ggtggctaca tgcttcggat ggaagaatac
    14341 ctggccgtgc acagtatttt tcttcgccaa gtgggacatc tttctgtttg gaagaaagac
    14401 tctttaaagc tttattttca aaatcctgaa aaatacagtt tttcaccgga agaacagcaa
    14461 aagatcaaga gggctcttgc acccatactg aaagctgaag acagagttat tcccgtattg
    14521 agcgggaccg gcatgatcac caatggatgc caaaatttac cgattatttt tgccgggatc
    14581 gatcctcaag atagtgaatg gatttatagt catccccaag ttcatcagca gattcctgag
    14641 ctcgtgcatt ttgatcaggg ggtaggctac tgggaaccag agggcagtct gatgcccatc
    14701 aatttaagtc ctattctctt ttcattgcta aaaaagaaga atttattcag tggtggaact
    14761 ttatctgcgg gagaagtagt caattgcagc catccggatt ctttaatcaa gaattccgct
    14821 gatccttccg tgcaaatatt ttcagcggca tttgatggtg ggattggaat tactgacgcc
    14881 ctcctagttg gtttaaaatc aacgggtttc actttttccg acgaaaccgc agtgatgcta
    14941 tctcgggatg tcgctaaaaa ccttttacgc accgacaatg tttcgcgcat ggtgattatg
    15001 ctggcaaatc cggaaaattc gaaatccttc cgcgtcgccg tggcaggggc catagaaaaa
    15061 gccggagtcg ataattttga agtgcttcat tataccgatg aatcggtcag ccctatctat
    15121 gtcggcgcaa tgaattttgt ctacgtcatg atgggtttct ttgtggtttt agtttgcgga
    15181 gtggtcattt tgtcgatcgc gaattctttt caaatcgcgt ttatggaacg aagacatgag
    15241 tttgccaccc ttcgctctgt ggggtttcgt tctggggttt taagaaccct tttaatgttt
    15301 gaatatattc aaatggccgc gatcagtttt gcggtgggcg ggtgtttggc tttcatttta
    15361 aaagggctga acaatgcctt gaatctgcga tttcagattc cgggttttgc caatgacttg
    15421 caattcaaat tggaacccac ttttattttt acctattcag tggctttagc catgatgatc
    15481 attgtcgttg tttcggtcca cttttttgcc cgccagcaaa tgaaactcaa ggtcattgag
    15541 ttgatgggga ctgaggcatg attttattca aaattattca gctggctttt aaaaatatca
    15601 tgaactcccg taaaaaaagt ttttccgcca tcctctccgt ggccagtggg tttttagcgc
    15661 tgaacctttt tcaggcttac attgtagata ccaatatttt atttgatcaa acttatgaga
    15721 atcgctctat gctgggccac cttctcatca gaaaagaaag tcgcctggac ctaaccacgg
    15781 cggaagaaca aaaagaaatc atcaggtttc taaccgagaa tgggaatgaa attgaggtgg
    15841 tggttcgatt tttgaacttt accgggactc tgaattcagg atccgtgcaa acggttttct
    15901 ctggctacgg ctatgatatt gatcaaggaa gaaaaattcg gggtgcttct tggcagtgga
    15961 atgtagtagc cggagagccc ctggatcaag ctccaaacac ccggggttta ttgttaggaa
    16021 gacggcttgc ggaaaccgtt aaatgcaaag agaatcaacc tctggatgtt ttaattagca
    16081 aggctggtta tgaggcaaaa actcgcggat ttcagtgtga aaatccgagt cttcaacttt
    16141 caatttctac gcaaacgggg caggtcaatg cggctgaact taaagttttg ggaattttag
    16201 atggagtgtt ccgggatatt gatgaaagac tggtggtcac ttctctggcg gaggcccaat
    16261 cccttatgca ctccgatgaa atttcgatga tcgctcttcg cttgaaagag gttggcaaag
    16321 gagatgagct gaagagccgt ttttcacaaa cggttcagaa aaagcatcct cagttgcaat
    16381 tgaaatcatg gaaagaatat gagatcggtg acttttataa caagagcatc aatttccttt
    16441 ttatattcag aaattttatc acactggtgg tgatctcgat tggagcgcta tcggtgatga
    16501 cgacctttta ccgtctggtt tttgagcgaa ctcgtgaaat tggcactttg ctcagcgtgg
    16561 ggtttaaatc aaagcatatt ttcacgctca ttatttcgga agcatttttc ttaagtttac
    16621 tgggaatagc catcggtgtg cttgcctcct atatagtcgc ggccctggtc aattcattgg
    16681 aaattcccta taagctggga atgctttctg aagcggtgcc atttgcgatg acgattcagt
    16741 ttaaaatgct gtttttatcc gccctgacac tgatttttat ttccattggt atttgcgcat
    16801 ttccggtaaa acgagcctta aaaatgaagg tctccgatgc acttagggag tagtcttaat
    16861 cactccatcc acgatccaag agaggttttt cactcgttgg atcagctttt catcatgagt
    16921 cgcaaacaaa aaagtgatgt tccgtttttg attgagatcc aagagaagat cgatcacttg
    16981 gtgggccgtg gcggaatcca aattggccgt gggctcatca gcgagaatta actccggctg
    17041 aggaactaaa gctcgggcaa tggccactcg ctgcctttgg ccccctgaaa gttgatcggg
    17101 cttgtggttc atatgttcat taagacctac atcgttgatg gcgatccgaa tccgttgttc
    17161 tttttcctca tcgctgagat ctgatcgcag aattaaagga agttcaatgt tctgtttcac
    17221 attcaaagcg ggaatcaaat taaagttttg aaatatcatt gatattttgg aattgcgtag
    17281 atcacttttt tggtcctcgt tcaaggtggc taagtcctgg cccataaata aaaggcgacc
    17341 ttggtctgga gtatccagcg tggctaatag atgcagcaaa gtacttttgc ctgagcccga
    17401 agcgccgagc aaggcccgga aatcgccttt ttggactcta aagttaagac ctctgagggc
    17461 cgggactttg gtttcgccaa gctgataggt cttataaaga ttttcaattt gaatcatgag
    17521 cggtgacctc taaaaggaaa attttaaaaa ataactggca taggagtgca tataactgct
    17581 gagttcactc tcattgtcac cggaagtggc gataaaggca atgctatgag aaagttgatc
    17641 ggttaagctg gtctcgatgg caaaattgaa cagttgggat gggtctgtga gagaaaaaat
    17701 atttcttaaa taaatatgag agttcgaaaa gacagcctct aaaaaaggag gattgtccca
    17761 gcggaaagaa ttatagatga attcttgtcc tggaagaggg ctgacgctgt cgttcaggat
    17821 agaaaggtac tgagtagcca gaggtgtggc atggattaaa cgataagttc tttgcctttg
    17881 ctccttcgaa ggtccgaaat cattgtgaag gtattctatg cgccattcaa tgccattgac
    17941 gaaagtaaaa cggtgaccaa cgagacttac ttgaaaggca gtggagtcgt cagcataagc
    18001 gggcagaaga gttccagtat catcaatgta aagcctttcg gagccttgct gaagaccgat
    18061 atcataatag atctgagtga agttggtgta gttccacatg gcataagagc cagcttgagc
    18121 gcgttcacga tccgtgtgtt tacggccgag aaccaagcct aagttaaagt ccccggagcc
    18181 ggtggcaaat tccccccgta aaagaactct tttttttgaa gtgcggtctc tgtacggagg
    18241 tgtatccgta tgttcccaac cgaaggggtc aagctctccc atcccagtga gagtgaagtt
    18301 ttgattagga gtccactgga ccacagccat ttccacgcct tgagtgcttt gaaatggctc
    18361 gagcttaatc agcaaatcag aaaataaaaa ttggctggga cttaaaatat ccgagggtcc
    18421 ccattgagaa ttaatgatgc ccacagagag attcacattc tttattaact cccaatgtgt
    18481 ataggcttcc cagaagccgt agtcagcaag acgttgggat tcattgaccg agccaaagtt
    18541 gtatctcgcg ctgtgaaact gcaaacgagg gataagcacg accttaagat tgtagtgggt
    18601 ggagtcaatt ttcagagtcg gtttaatttc gacatcaaac gcttgggagg gcagatataa
    18661 aagctgattg ttaggattgg tgatatcctc gttgttgtgc tggagaaatc ctccagtaag
    18721 aaaatcggac ctgaagtcaa agttgaacaa aggcccggct aaagcactag aaaaagcaac
    18781 gagggaaaat agacaaatac taaatgttct aaaggctaac agtttcattt cgtcaagtta
    18841 ctgacattaa aatccgcatc atttaaactg gatgcattgg gtttgctgta gatgagttct
    18901 gatttcagct cactgttttt cgcatcgacg atgatggttt tgcttacaaa gggaagggtt
    18961 tttccattat aagaaacgga attatcatag ctaaatttgg cctttttgaa gacatcgccg
    19021 tccagagtta agaactcggc ttggattccc aagagtttgt cctggctgat ccaatagaca
    19081 atttgatcgt aggtaagatc gttgcttttg gcttttaatt tcaatttcca agctttaacg
    19141 ccatcgacag cttcttctcc gctcaaggtg ggggtgtagt ctcgagcata attggtcgtt
    19201 gcgatgtcgc cattcgcagc ctgacctgaa agtctttggc gagaagacaa agagaggggt
    19261 tttctcaggc cgggtctatg aatccataag tttcgatcta aaaataagta gacttcatct
    19321 ttcgaacggg ctggcgcgat acattttgcc agaacattgg aggctttgac tttaacatcg
    19381 tattcaaagc tattttcttt tgctccatct tttgaaacga gctgcaattt ccagctcagt
    19441 cctcccgaca atccaccgcg ggcgcgatcc gcggctttta aaagctccac cgcatctgga
    19501 gttttaacgg cataggtttg aaatgaaaga aaaaaaagag tcgcaaataa caatgttttc
    19561 ataggaaaca gttaacaatt agtttttgtt aagatcaagc attgggagct acttttaatt
    19621 aaaattttat ttcatttagc agtccgaatt ctgtatttcg gcaacgaaac ggggtggaat
    19681 ataaaggcaa tttataatcg cgcgatgaga atcaggcatc cttttgcgaa agaaaatcaa
    19741 aaaatcgtga tcatcgtcgg cggaggattc gcaggactgg ctgcggccaa catgctggct
    19801 gaggagcctg aagtctatgt tattctcata gacaagcgaa accatcacct ttttcaacct
    19861 ctcctttatc aagtggcaac aggggggcta aatgcttcag atatagccgt gcctattcgt
    19921 tctcaattct ccaaggtttc caatgtcgaa gttcatatgg ctcaggtcga ggccgtcaat
    19981 gtccaggaaa gctttatcac taccgaaaga aatgaaatac tttttgattt tctcatactt
    20041 gcctgcggag ctcatcatag ctatttttcg aatacagaat gggcggcttt tgctccgggc
    20101 ctgaaaaccc tggaggatgc tatagatatt cggggccgta tttttcgcgc ttttgaaaga
    20161 gctgaaaatg agttggatct ggaaaaacaa agggcgcttc ttaactttgc catcgtggga
    20221 ggaggcccca ccggcgtaga gcttgcgggt gccattgcgg atgtggcaag gacggtcctt
    20281 atcaaagact tcaaacgtat taatccggcc agcgctcgga ttctattatt agaagcgggg
    20341 ccgaaaattt tatctgcttt ttctgaggat ctttcacgcc atgcccgcaa tgatttagaa
    20401 aagctcggag ttgaagtcag gacgggggct aaggtggaag ccatcacgga ttatggttta
    20461 ataatcggtg gcgaatttat aaatacctat aatgtttttt gggcggcggg agttcaagct
    20521 gagaaaatga atatcacccc gtcagtggca atggacgctg tcaatagaat caaagttgaa
    20581 aaggattttt caatccccgg ttttccctat tgttttgttg ttggagatat ggcgaccatg
    20641 cagctggaaa acaatcgccc gcttccgggc cttgccccag tagctatgca agcgggaaaa
    20701 catgccgcaa aaatgattaa gagttccttg caaggtctgg cccgcgaacc ttttcattac
    20761 gtggataaag gattgatggc gaccatagga aagcgaaaag cgatttcgca gtataagtct
    20821 ttaaaaatga ctggctatat agcctggctg gcctggcttt ttattcatgt atattattta
    20881 gtgggattta aaaacagggt ttttgtcatc attcagtggg cttggagcta cgttttttcc
    20941 aagcggggag cacggttgat taccggaaaa gaaagtcctt cggcttcatc cactatttcc
    21001 tagcgggcta ggaaatgaac aaaataatca attcactctg tggcttaatt tcaatgtctc
    21061 cagcattaaa ttgtctcatt ttgagtcatg gtaagatctt tgcatgatta aaagatatga
    21121 agaacctatt atggataagc ttacttttaa ttctcaat
//
LOCUS       DATBTE010000096         3545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2264985, whole genome shotgun sequence.
ACCESSION   DATBTE010000096 DATBTE010000000
VERSION     DATBTE010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2264985"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VIG33_03865"
     CDS             <1..295
                     /locus_tag="VIG33_03865"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8269998.1"
                     /translation="TFITRFPSSPYADNVLYLAGRRAFSAKNYAEAIKYYQKVITQYS
                     RSNKAVAAKFAKAVTYKKMNLEPQAEGVLNDLRRRYPGSPEAFRAENELKLMR"
     gene            323..1729
                     /locus_tag="VIG33_03870"
     CDS             323..1729
                     /locus_tag="VIG33_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8269999.1"
                     /translation="MQKVLLKIVLMILVIPLYVRAQDADPDFKSEERFHRIYKKYNQE
                     PTSEEAWEKVLSGRHANTYSIQDRDTLWDISHTLFGDSHYWPKVWSYNTDDIQNPHQI
                     NAKQGIKFYPGTLAEAPTIGLANKSDAAEALPTHVLEKNQEGKLEGFKIPFSKRKSRP
                     LVKKLPDSLPLYRLGAVNKPPLDFEISGARAKYSASLKYLSFYITEKPLESVGEVVEM
                     EHLDEQTSGDYEVIIVRVSNASGKRFVAFKDNTQVSEASELGATKAWSVEVLGEVEIQ
                     ERVNENDNLFRAIVKKSINPLEIGAKLLPGSMSTFDASPSTLTSSVQARIIGGEYARF
                     DQKMFGTDHIVFLNAGAQEGLREGSSLPIFLNERLRKPETKAVANDRIIGEIKIIKLA
                     GHFATGYIVDSQSPVFVGDYVGGRVKSSAGGSAAAAEATDENGNSKNSDFDLDLDVPS
                     QKDNGGENPADADDELHL"
     gene            1854..2777
                     /locus_tag="VIG33_03875"
     CDS             1854..2777
                     /locus_tag="VIG33_03875"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY8270000.1"
                     /translation="MEKLIHFSLIHKNLGLLQNYRRLRESIEAMSISCSCEELRAYLR
                     EQEPFFFREVCERPSTLQRESEELTRLKNRGVNFTYIGESSYPISFHNMPGAPLFLSY
                     RGHPTWLRGKSLAVVGSRDASQLSHLWMEESFAEFLRQEKPVVVSGGARGIDQMAHQL
                     ALRNSCSTVVVLPSGLEFLYPKTLEAWQAKIIEGGGCLLSEYSYSEKMQKHFFHDRNR
                     LIAGLGVMTLLVESRRRSGSLLTAQKTLQLGKTVLIVPGHPCDNSHLGNLDLLSEGAT
                     PIRDAEELCMYFRSELSLLSKFIPIASEIQF"
     gene            complement(2991..>3545)
                     /locus_tag="VIG33_03880"
     CDS             complement(2991..>3545)
                     /locus_tag="VIG33_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY8270001.1"
                     /translation="ERDRSAPLTIIAKRGAIVRDPKSPGHNVLLRLENGDIHRKTQTH
                     TKIKFDTYDIRLIDPIRDIIREKSVQSLTLSELHETLKKPDLTVKDRRTYQTEFHKRW
                     AISVLCLVFALIGVGLGTTTNRRAAKAGGMIMCIGIIISYWVLYVTFESMARNGQMPP
                     AVAVWSPIILFSAIGFESLRRNWN"
BASE COUNT          964 a          761 c          885 g          935 t
ORIGIN      
        1 aacatttata actcgctttc cgtcgagtcc ttatgcagat aatgtgctct atctggctgg
       61 tcggcgtgcc ttcagcgcca agaactatgc ggaagcgatt aagtattatc aaaaagtgat
      121 cactcagtat tcacgcagca ataaagccgt ggctgccaaa ttcgccaaag ccgttactta
      181 taaaaaaatg aatttagagc ctcaggccga gggagtctta aatgatttgc ggaggcgtta
      241 tcccggcagc cccgaggctt ttcgcgcgga aaatgaactt aagttgatga gatagtttct
      301 ctactttcca aggatggcac gagtgcaaaa agtactactt aaaattgtcc taatgatttt
      361 agtaatcccc ctgtacgtcc gtgctcagga cgccgatcct gacttcaaaa gtgaagagcg
      421 ttttcatcgc atttacaaaa agtataatca agaaccaacc tcagaagagg cctgggaaaa
      481 agtattgtcg ggtcggcatg cgaataccta ctccattcaa gacagggaca ccctttggga
      541 catcagccat actctttttg gagactctca ctattggccg aaggtttggt cttacaatac
      601 ggatgacatt caaaatcccc atcagatcaa tgcaaagcaa gggatcaagt tttatccagg
      661 aactttggcg gaagccccca cgataggact cgccaataaa agtgatgccg ctgaagcttt
      721 gcctactcat gtattggaga aaaatcagga agggaagttg gaaggtttta aaattccttt
      781 ttcaaagcgg aaaagccgcc ccttggttaa aaagttgcca gatagtttgc ccttatatcg
      841 tttaggagcg gtgaacaaac cccctctgga ttttgaaatt agcggtgccc gtgcgaaata
      901 ttcagcttcg ttaaaatacc tgtcatttta tattacggaa aagcccctcg agtccgtcgg
      961 tgaagtggtg gagatggagc atctcgatga gcagacgtct ggggattacg aagttatcat
     1021 tgtgagagta tccaatgcgt cgggaaaacg atttgtagcc ttcaaggata acacccaagt
     1081 gtctgaggct tcggagttgg gggctacgaa ggcatggtcg gtggaagtgt tgggtgaggt
     1141 tgaaattcaa gaacgagtta acgagaacga caatcttttc cgtgctatcg taaaaaagtc
     1201 gatcaatcct ttggagatag gtgcaaagct gcttcctgga agcatgagca catttgatgc
     1261 gtctccctcg actcttacct cctcggttca agcacgaatc atcggaggag agtatgcaag
     1321 atttgatcaa aagatgtttg ggacggacca catcgtcttt ctgaatgcag gagcccagga
     1381 agggctccgt gagggctcaa gcttgcctat ttttcttaac gaaagacttc gtaagccaga
     1441 aaccaaagcg gtggccaacg acagaatcat tggagaaatt aaaatcatta aactggcggg
     1501 gcacttcgct acaggataca tcgtggattc tcaatctcct gttttcgtcg gtgattacgt
     1561 cggcggccga gttaaaagtt cagccggcgg ttcagcggct gcggcggagg cgacggatga
     1621 gaatggcaat agcaagaaca gcgattttga tctagatttg gatgttccaa gccaaaagga
     1681 taatggtggt gaaaatccag ccgatgccga tgacgaattg catttataga tttacttatg
     1741 attactagat tacgaaatga ccgaacaagt gttcgaattc tgaccgctca aagactcagc
     1801 ttcgcaataa aagcattttg aagctgagtc tatctttgca ggttcagctc gttatggaaa
     1861 aactaattca ttttagcctt attcacaaaa acctcggttt gttgcaaaat tatcgtcgtt
     1921 tgcgagaaag tatcgaggcc atgtccatat cgtgttcttg tgaggaactg cgtgcttacc
     1981 tgcgcgagca ggagcctttc ttctttcgcg aagtttgtga gcgcccatcc acgcttcagc
     2041 gcgaaagtga ggagctaaca cgattgaaaa atcggggagt gaatttcact tacatcggtg
     2101 agtcgtccta ccctataagt tttcataata tgccgggggc gcctttgttt ttgagttaca
     2161 gaggtcatcc gacgtggtta agggggaaat ctttggcggt ggtgggtagc cgcgacgcat
     2221 cgcagctttc gcatttatgg atggaggaaa gttttgcgga atttcttcgc caagaaaaac
     2281 cggtggtggt cagtggagga gctcgtggca ttgatcaaat ggctcaccag ttggcgctcc
     2341 gcaattcctg ctcgacggtg gtggtgcttc cttctggtct tgaattcctc taccccaaaa
     2401 ctctggaagc gtggcaggcg aagattatag aggggggagg ttgtttattg agcgaatatt
     2461 cttactcaga gaaaatgcaa aaacatttct tccatgatcg caatcgcttg attgctggat
     2521 tgggagtcat gaccttgctg gtggagtcgc ggcgtcgaag cggaagtctg ctcacagcac
     2581 agaaaacttt acagctggga aagaccgttc tcatcgttcc cggtcatcct tgcgacaact
     2641 ctcatctcgg caatctggat ttattgagcg aaggggccac tcccatccgc gatgctgaag
     2701 aactatgcat gtatttccgg agcgagttaa gtctattatc gaagtttatc cctatagcta
     2761 gtgagattca gttctgattg gtctatacct aggaaatcta attcgccaat ttaagttatc
     2821 taaaaaaaat gaaaaaagcc tttgaaaaaa ccttagcttt gctcgtaaca tgagaagtaa
     2881 gggaggggcg atgtggtcaa ggatgaccgt agtggtaagg acactgctgc atcgctctca
     2941 tttttagaat agtctgaagg cttcttttct ctgttcaaga aaccattaaa ttaattccaa
     3001 tttcttctta acgactcaaa gccaatagcg gaaaatagta tgatagggct ccaaacagca
     3061 accgccgggg gcatctgacc atttcgtgcc atgctctcaa aagtgacata gagaacccaa
     3121 taactgataa taatcccgat gcacataatc ataccgccag ccttcgccgc gcgccgattg
     3181 gtagtggtcc caagacctac tcctataaga gcgaaaacaa ggcacagcac cgagatcgcc
     3241 cagcgtttat gaaattcagt ttggtaggtc cgacggtctt tcaccgtgag gtctggcttt
     3301 ttcaaggtct catgcagttc agataaggtc agggactgta cggatttctc acggatgata
     3361 tcccgaatag gatcgatcag ccggatgtca taggtgtcga atttgatttt agtatgagtt
     3421 tgggttttcc tatgtatatc gccgttctcc aagcggagaa gaacattgtg gccgggagat
     3481 ttggggtccc gtacaatggc gccgcgcttg gcaataatag tcaaaggtgc actgcgatct
     3541 cgttc
//
LOCUS       DATBTE010000097         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2402093, whole genome shotgun sequence.
ACCESSION   DATBTE010000097 DATBTE010000000
VERSION     DATBTE010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2402093"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VIG33_03885"
     CDS             <1..1248
                     /locus_tag="VIG33_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270002.1"
                     /translation="RSVLLAGLFLLNLSMISCATYQGKVAESRQHLASGQYAEAEKYL
                     EPLANKENGDQLVYLLDYSVALQMGGKIKESAQALQRADRLAEFQDFTSLSRETGSLL
                     FNQEMVQYKGDSFEKIYINAYAALNYLELNQLDEALVETRRMNEKYNKLNSEDRKTFE
                     KNVFGKYLSAMIWEANQNWDDAYIAYEETYKLDPNISFLEEDLIRSAKRARRMDTYQK
                     WKKAFPHVVEKKEWYDKNHGELIIIYEQGWGPRKVQVGGQGIYAGGYNPPVLQPVYNS
                     ILQMKATISGDRQVASRVVYDVEKAAIETLQDDYAWLVAKRMGSFAAKEIVADQIRQK
                     NEALGSVAWLAMHLAERADLRQWSTLPQTVQLVRVPLKAGEYNVNLQGLTRNGEASGE
                     GKAFSKIQIKSQHATFLRWRTLN"
     gene            complement(1326..1877)
                     /locus_tag="VIG33_03890"
     CDS             complement(1326..1877)
                     /locus_tag="VIG33_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEY8270003.1"
                     /translation="MINQNETLTSKQNSKVKIIKRYQNRKLYDTQQSCYVTLDDIAKM
                     IRTNEEVMVIDNKSKNDITAATLTQIIFEAEKKASQYAPLFTLREIIQNGNGSISNYL
                     AKLGAFPQDYMTKQAANIANIERASVDDVKQNLENRVANAATRYNPDTTAKAAAEKAT
                     VLPGAQGEEETPNLPSSNPSLNN"
     gene            2395..>2736
                     /locus_tag="VIG33_03895"
     CDS             2395..>2736
                     /locus_tag="VIG33_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270004.1"
                     /translation="MISAKRHAEVLGYQIFEIYQEGGRNSMGSEILNSRSPASMRTTG
                     GGSFGFSEVGSIGADPWSQPYRYKVLSSTGGDQLKVQIWSAGPNRVFETKDQPGVGAE
                     TYTGDDVGIILS"
BASE COUNT          777 a          569 c          629 g          761 t
ORIGIN      
        1 agatccgttc ttttggcggg tctttttttg ttgaatctaa gtatgatttc ttgcgccact
       61 taccaaggca aggtggcgga atctcgtcaa cacctggcaa gcggacagta cgctgaggcc
      121 gaaaaatatc tagagccttt ggctaataaa gagaatggcg atcaattagt ttatcttctc
      181 gactactctg tagctttgca aatgggcggt aaaattaaag aaagcgccca ggcgctacaa
      241 agagccgatc ggttggcgga atttcaagat ttcacttcgc tttcccgtga aacgggatct
      301 cttctgttca atcaagagat ggttcaatac aagggcgatt cctttgaaaa aatttatatc
      361 aacgcttatg cggcccttaa ttatttagaa ctcaaccaac ttgatgaggc cttggttgaa
      421 actcgtcgca tgaatgaaaa gtataataag cttaatagcg aagaccgtaa gactttcgaa
      481 aaaaatgtct tcggtaaata tctctctgca atgatttggg aagcgaatca aaactgggac
      541 gatgcttaca tcgcttatga agaaacctat aaactcgatc caaatatttc ctttctcgaa
      601 gaagatttga tccgttctgc caagcgtgcc cgccgcatgg acacttatca gaagtggaaa
      661 aaagcctttc cccatgttgt tgagaagaaa gaatggtacg ataaaaacca cggtgaactc
      721 ataattatct acgaacaagg ttggggcccg cgaaaggtgc aagtcggtgg tcagggaatc
      781 tatgccggag gttacaatcc tcctgtgttg caaccagttt acaatagtat tttacagatg
      841 aaagccacca tctccggaga caggcaagtc gcgagtagag tcgtttatga cgtagaaaaa
      901 gcggcgattg aaaccctgca agacgactat gcctggttag tggcaaaacg catgggttct
      961 tttgcggcaa aggagattgt cgctgatcaa attcgccaga aaaacgaggc tcttggcagc
     1021 gtcgcgtggt tggcaatgca tctggcagag cgcgcggacc ttcgccaatg gtcgacactt
     1081 ccccaaactg ttcagcttgt ccgggtgccg ttaaaagcgg gcgagtacaa tgtgaatctt
     1141 caggggctta ctagaaatgg cgaagcgagc ggagaaggaa aagccttctc gaagattcag
     1201 atcaaatccc aacacgctac atttttgcgt tggcgcacac ttaattagtt cgccaagttc
     1261 attaaaaaag gccctgattc ctcagagcct ttttgaacta cgaaaagctt gtaaaagatt
     1321 ggattctagt tgttcaagct tgggttacta gaggggagat ttggagtttc ttcttcaccc
     1381 tgtgcaccag gaagaacagt tgctttttca gcagcggctt tcgcagttgt atctggattg
     1441 taacgagtgg ctgcgttagc aacgcgattc tcaaggttct gttttacatc atcaactgaa
     1501 gcacgctcaa tattggcaat attagcagct tgcttagtca tgtagtcttg aggaaatgcg
     1561 cccaatttag ctaggtagtt ggagatgctt ccattaccat tttggatgat ttcacgaagt
     1621 gtgaaaagag gagcgtattg agatgctttc ttttctgctt cgaaaataat ttgagtcaaa
     1681 gtagcagctg taatgtcatt tttcgattta ttatcgatga ccattacttc ttcatttgta
     1741 cgaatcattt tagcgatatc atccaaagtc acataacagc tttgctgagt atcatagagc
     1801 tttcgatttt gatagcgctt gataattttg acttttgaat tctgtttaga agtcagtgtt
     1861 tcgttttggt tgatcaaagt gcctcccatt ggaaagtcta ctcggtttta taacttttct
     1921 ttcagaagcg ccttaaaaat aggcagttcc taaccccttt taaatccctt actacgccca
     1981 agaaacctac acagaaacat gttttattca gcattctgct gactcattaa gcggcagttg
     2041 gcggcaaatt aaccttgccg ccaccccttg tcaagcggtc gcaaccccaa ataaaaaaac
     2101 accatgcggc aaatccctcc cttcaaattt cgacaaaaaa caggattttc ggcccagtga
     2161 aaagctcaag agggaaggat aagaaatgcg aataagttta tttttgcctc taaagtttag
     2221 ttctaaacta gccgaaatgc tagatatcac aaactcttgc aagctggtaa tttagtgaaa
     2281 actgaagaaa aacatacttt tagctacaag aaccttgttt ttttgtctag ccttgcaggg
     2341 ttattgggct ttgtagcttt gccgagttgg acaagacaaa gagatgaaga gcgtatgata
     2401 agcgctaaac gacatgccga agttctggga taccagattt tcgaaattta ccaggaaggg
     2461 ggacgcaatt cgatgggttc agaaattctt aatagccggt ctccagcttc gatgcggacc
     2521 accggaggcg ggtcttttgg attcagtgaa gtcggtagta ttggagctga tccctggagc
     2581 cagccatacc gttataaggt tctcagctct acaggtgggg atcaactcaa agttcaaatt
     2641 tggtctgccg gacctaacag ggtctttgaa accaaggacc agcctggcgt tggagccgaa
     2701 acctataccg gcgatgatgt aggaattatc ctttct
//
LOCUS       DATBTE010000098         4763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_830599, whole genome shotgun sequence.
ACCESSION   DATBTE010000098 DATBTE010000000
VERSION     DATBTE010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830599"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VIG33_03900"
     CDS             <1..587
                     /locus_tag="VIG33_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEY8270005.1"
                     /translation="NSLVLGIPRGAMPMARIIAEGLDGELNAILIHKIPAPNQPELAI
                     GSVGLSGDIYRLPLIETYEIPEFYVQQAAHQQLEILKKRSERFHLPPLVCKDRIVIIV
                     DDGVATGATVFGAIHEIRQQAPRKLILAAGVVAKSTAEEIRPLVDELISLNEPEYFYA
                     VSQFFEDFSQVTDEEVVEILKSAKKSKRQTEVNI"
     gene            complement(584..1024)
                     /locus_tag="VIG33_03905"
     CDS             complement(584..1024)
                     /locus_tag="VIG33_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HEY8270006.1"
                     /translation="MKINKFIAQSPIFALYRGNSLLVEPFQMRLAKEGVHLLQGLILT
                     ALFFEEREVRPFELAKAFQVSKSNLSHSLRSLEKKGWLKRAMHSEDARGYLFSLTASG
                     KKKALILIKSFDEVEGELEKKIGTKNTKEFVNVLNQFVTDYRSN"
     gene            1125..2072
                     /gene="bioB"
                     /locus_tag="VIG33_03910"
     CDS             1125..2072
                     /gene="bioB"
                     /locus_tag="VIG33_03910"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEY8270007.1"
                     /translation="MENKEFEDIQEVFERPLLELVTKAHSIHRERFKADEIQASSLLS
                     IKTGGCPENCSYCPQSAHYSTGVEKTKLLNKETVVSAALAARALGASRFCMGAAWRNV
                     KDGSEFDEVLELVKAVKHLGFEVCCTLGMLNQMQATRLKEAGLYAYNHNIDTSRNFYT
                     KIIQTRTYEDRLKTIDNVRSAGLTVCTGGILGMGESSEDRVAFIHQLASFKPLPESIT
                     INTLVPFGGTPLANQDSLSPLEIVRMVATLRIVAPSSMIRLSAGRLSLSEEAQFLCFM
                     AGVNSIFIGDKLLTSPNPQAVQDQDLLRKLGYSLKKATN"
     gene            2073..3206
                     /locus_tag="VIG33_03915"
     CDS             2073..3206
                     /locus_tag="VIG33_03915"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEY8270008.1"
                     /translation="MDDIHKEIQNWEIRGLLRELVHSSGIDFASNDYLGYSHNPVIHA
                     KLVDFIKDHPKIGSTGSRLISGNSQHIEETEDFLAHCFSTEASLIFGSGYLANMGVCA
                     ALGGAETEFFSDELNHASIVDGIRISKSKCHIFRHNDLEHLNYFISKSSARRKVIVTE
                     SIFSMDGDFASLEQLLEIANKYDAYLFIDEAHATGVCGIQGLGRMLEFKFPSAKTILV
                     HTCGKALGAYGAFVCGTSAIKRLLLNKARSQIFTTALPPFAVEHIRLAVAHLISDPLS
                     VEKLKANILYSKGLFLRYQLKHSGSHIVPFLLGSNRAVLQSSQRLEKQGVFVKAIRSP
                     TVRAGSERLRLTFKSTHSFEDIDKLCKILWELNSECKYIHYGN"
     gene            3175..3843
                     /gene="bioD"
                     /locus_tag="VIG33_03920"
     CDS             3175..3843
                     /gene="bioD"
                     /locus_tag="VIG33_03920"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEY8270009.1"
                     /translation="MNANIFITGTDTSVGKTLVSATLCLKLGWKYWKPLQCGFSEEAS
                     GATDSDWVRNYIDPGKVYPEAYRFCSALSPHAAAAIEDRTIHLELLLKKGQQMNHTII
                     EGAGGLLVPLNQELLLIDLIQKLKCQTLLVVRSGLGTINHTLLSIEALRSRHIEPLGI
                     IMVGDPNPANRQAIQYYGNVKVLGEIPMLQNINRPALCEAGKLINLKENFNEYCEHAQ
                     GHCR"
     gene            3806..4414
                     /locus_tag="VIG33_03925"
     CDS             3806..4414
                     /locus_tag="VIG33_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270010.1"
                     /translation="MNIVNTLKVIVDNPRIHACWLNSLSYLEYRGFRKIIRSRTTKEI
                     SLQILIHSAEEVRHSVFFKRQAVKIGGNDFIHFAEESLLASQALKNYFYKIDARTMAA
                     IEGVQGSLLSEAAYSVITWLVETRALSLYEEYEKILRASSAQFSLSSILREESLHLKE
                     IGISAARFLLEQGFDLQFIAAIEKSEFEKLWIAIQESFEVRP"
     gene            4411..>4763
                     /locus_tag="VIG33_03930"
     CDS             4411..>4763
                     /locus_tag="VIG33_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8270011.1"
                     /translation="MNIWRPYTQMYRAERQEMVMSARGSYLYLKNGQKIFDGISSWWV
                     ITHGHCHPEITEAISSQTKKMDQVVFANFTHEPVEMLVQELSDILPEKLDSVFFSDNG
                     STSVEVAMKMAYQYC"
BASE COUNT         1333 a         1000 c         1108 g         1322 t
ORIGIN      
        1 aaaactccct tgttttggga attccccgtg gcgctatgcc catggctcgg attattgctg
       61 agggactgga tggagaactc aatgcaatat taattcacaa gattccggca cccaatcaac
      121 cggaactggc cataggatcc gtgggcttat cgggagacat ttatcgactt ccgttgattg
      181 agacctatga aattccggag ttttatgttc agcaagcagc gcatcagcaa ttagaaatcc
      241 ttaagaagcg gagcgaacga tttcatttac cacctttagt ttgtaaagat cgaatcgtca
      301 tcatcgttga tgatggtgtc gccactggtg ccacggtctt cggtgccatc catgagattc
      361 ggcagcaggc gccgcgaaaa ttgatcctgg ccgccggggt ggtcgctaaa tccacggccg
      421 aagagattcg acctttagtt gatgaactca tttcattgaa tgagcctgaa tacttctatg
      481 cggtcagcca attttttgaa gacttttcgc aagtcaccga tgaggaagtg gtggagattt
      541 taaaaagtgc taagaaaagc aaacggcaaa ctgaagtaaa tatttaattt gaccgatagt
      601 ctgtcacgaa ttgattgaga acattcacaa attctttcgt gttctttgtg cctatttttt
      661 tttccagctc accctcaact tcatcaaagg attttataag aataagggcc tttttctttc
      721 cggaggcagt caaagaaaaa agatagccac gcgcatcttc agaatgcatg gctcgcttaa
      781 gccaaccttt cttttctaaa gatctgagag agtggctaag attggatttg gaaacttgaa
      841 atgcctttgc caattcaaaa ggccttacct ctcgttcttc aaaaaagagg gcggttaaaa
      901 tcagcccctg taaaagatga acgccttcct tcgccaagcg catttgaaag ggctccacca
      961 gcagggagtt tcctcgatac aatgcgaaaa taggactttg cgcgataaat ttgtttatct
     1021 tcatttgcaa ggaattagct taattatatg aatagttcaa ctattgaata tagaatatcg
     1081 tagcaaagca ttgacactct tgttgagcgg tttcaaatca cggaatggaa aataaagagt
     1141 ttgaggatat tcaggaagtt ttcgagcggc cgctgttgga gttagtcaca aaggctcact
     1201 caattcatag ggagcggttt aaagccgatg aaattcaagc ctcctccctt ctttcaatca
     1261 aaacaggggg ctgtcccgaa aactgttctt attgccctca gtcggctcac tactccacgg
     1321 gcgtggagaa aacaaaatta ctgaacaaag aaacggtggt gagcgctgct ttagcggccc
     1381 gtgctctggg agccagcaga ttctgtatgg gagccgcttg gagaaacgtt aaagatggat
     1441 cggaatttga tgaggttcta gagcttgtga aggctgttaa acatttgggc tttgaggtgt
     1501 gttgcacatt gggaatgttg aatcaaatgc aagcgacaag gcttaaagaa gccggcctct
     1561 atgcttataa tcataatatt gatacctctc gcaacttcta tacaaaaatt attcagacac
     1621 gaacttatga ggatcgcctg aaaactatcg ataacgtacg aagcgcaggt ttgacggttt
     1681 gcacaggcgg cattctgggg atgggggaaa gctccgaaga tcgggttgcc tttattcatc
     1741 agctcgcctc ctttaagccg ctgccagaaa gtattaccat caacactctg gtaccctttg
     1801 gcggaacgcc tctggcaaac caagactccc tctctccact tgaaattgtc cgcatggtgg
     1861 ccactttaag aattgttgct ccgtcctcaa tgatccgtct gagcgcagga cgtttgtcct
     1921 tgagcgagga ggcccagttt ctttgcttta tggcaggagt caattccatt tttatcggcg
     1981 ataaacttct aacctcaccc aatccacagg ctgttcaaga tcaggatctt ctacgaaagc
     2041 ttggttacag ccttaaaaaa gcgacgaatt gaatggatga tatccataaa gaaattcaaa
     2101 attgggaaat caggggcctc ttgcgagagt tggttcattc cagcggaatc gattttgctt
     2161 caaatgatta tctgggatat tcgcataatc ctgtgattca tgcgaagcta gtggatttca
     2221 ttaaagatca tcctaagata ggatccaccg ggtcacgcct tatatctgga aattcgcagc
     2281 acattgaaga gaccgaagat tttttggcgc actgtttttc aacagaagcc agtttgattt
     2341 ttggatctgg ctacctcgct aatatggggg tctgtgcggc cttaggcggt gcagagacgg
     2401 agtttttttc tgatgaactg aatcacgcaa gtattgttga tggaattcga atttccaaat
     2461 cgaagtgtca catctttcgg cacaatgacc tcgaacatct gaattatttt atttccaaaa
     2521 gctctgcccg ccgcaaagtg attgtcacag agtctatttt tagcatggat ggagatttcg
     2581 cttcgcttga gcagctcttg gaaatcgcaa acaaatatga cgcttaccta tttatcgacg
     2641 aagcccacgc cacgggagtt tgtggcatac aagggcttgg tagaatgttg gaatttaagt
     2701 ttccctccgc aaaaactatt ttagtgcata catgtggtaa agcattggga gcctacggag
     2761 ctttcgtttg cggaacttct gcaattaaaa ggctattgct caataaggcg agatcccaga
     2821 tttttacgac tgctttgccg ccgtttgcgg tggagcatat tcggcttgct gtcgcccatc
     2881 taatttccga tccactgtca gtggagaagt tgaaggcgaa tatattgtat agcaagggcc
     2941 tttttctacg atatcaactc aagcattcag gatctcatat agttcccttc cttcttggaa
     3001 gtaatagggc agttttgcag agttctcaga gattggaaaa acaaggagta ttcgtcaaag
     3061 ccattcgctc gcccacggtg agggcaggaa gcgaaaggtt aaggctcact tttaaatcga
     3121 cgcacagctt cgaagacata gacaagttat gtaaaatact ttgggaattg aacagtgaat
     3181 gcaaatatat tcattacggg aactgatact tccgttggaa agactctcgt ctccgccact
     3241 ctctgtctga agttgggttg gaaatactgg aagcctcttc aatgtggctt ctctgaagag
     3301 gcttctggag caaccgacag tgattgggta agaaactata ttgatccagg aaaagtttat
     3361 ccagaggcct accgattttg cagtgctctt tctccccatg cggcggcggc gatagaagat
     3421 aggaccatcc atttagaact tctattgaag aagggtcagc aaatgaatca tacgattatt
     3481 gaaggagcag ggggccttct cgttccactc aatcaagaac tcttacttat agatttaatc
     3541 caaaaattaa aatgccaaac tctccttgtt gttagatcag ggcttggaac cattaatcat
     3601 actttgctgt caatagaagc gctgcggagc agacatattg aacctttggg cattattatg
     3661 gtcggagatc ctaatcccgc gaaccgtcaa gccattcaat attatggaaa cgtaaaagtg
     3721 ctgggtgaaa ttcccatgct gcaaaatata aatcgtcccg cattgtgcga ggccggaaaa
     3781 ttgataaatt tgaaggagaa ctttaatgaa tattgtgaac acgctcaagg tcattgtcga
     3841 taatcccaga attcatgcat gctggttaaa tagcctatcc tatctggaat atcgaggttt
     3901 tcgaaaaatt attcgttctc gcacgacaaa agaaatatct ctgcaaattc tgattcatag
     3961 cgcggaagaa gttcgccact ctgttttctt taagaggcag gcagtgaaaa taggaggcaa
     4021 tgattttatc catttcgcgg aagagtccct gctggcttcc caagctctga aaaattactt
     4081 ttataaaata gacgccagaa cgatggcggc aatcgaggga gtgcaggggt ccctgctctc
     4141 agaggccgct tattctgtga taacctggct tgtggagacc cgggctcttt cattatatga
     4201 agagtatgag aaaatactga gagccagctc agcgcagttt tcgctctcaa gtattcttcg
     4261 tgaagaaagt ttgcacctca aagaaatagg aatctcagcc gcaaggtttc tgctcgagca
     4321 aggatttgac ctgcagttca tcgccgcaat agaaaaaagt gaattcgaaa aactctggat
     4381 cgcgatacag gaaagctttg aggtgaggcc gtgaacatct ggcgtcccta tacgcaaatg
     4441 tatcgggctg agcggcagga aatggtgatg tctgctcggg gctcttatct ttatctcaag
     4501 aatggacaaa agatttttga tggaatttcg agttggtggg ttattaccca cggtcattgc
     4561 catcccgaaa tcaccgaggc tatttcctct cagacaaaaa agatggatca agtggttttt
     4621 gcaaatttca cccacgagcc tgtggaaatg ctagttcagg agctcagtga tatattgcca
     4681 gaaaaacttg attccgtatt tttttccgac aatggatcca catctgtgga agtggctatg
     4741 aaaatggcgt atcagtattg cca
//
LOCUS       DATBTE010000099         1566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1035707, whole genome shotgun sequence.
ACCESSION   DATBTE010000099 DATBTE010000000
VERSION     DATBTE010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035707"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /locus_tag="VIG33_03935"
     CDS             complement(<1..1503)
                     /locus_tag="VIG33_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8270012.1"
                     /translation="MLHRSTRPPFIVLALLLILSACATLQDSDTAPYYDAAFNDKNRA
                     PAAFTPHLQSGGEGTTTVLDPFYLRTQADYYFTVGESYSLDGDHSKAIDAFKMALLYD
                     QESPMVNIRLATEYFKTAQFTESLTQAEESIRKDPKNIEGRLLLGGLYLSLKLFPKAI
                     GQYEYVLKVQPHNVEAPVYLGAAYTEIKQFDKAVKFFESLIKNEDYSTPHLAYYYIGR
                     VRSEQKGEVFQKASMKAYQKAIELKPDFLDGYVAIASFYTQKGQEEKALALYRKFQKE
                     QGPNRKVAEILAQIYVESEQYDLAYEQYQILENTTEDSLNIKLKMALILIERKDFIAA
                     AAKLEDILRDAPESDKIRFYLGAVYEETKDYEKAIYQFKKIPTASQFFGEAMVHTAYM
                     MKNKGRLGDAVEVVESALKERKDQAQLYAMYASLLDERGEYNKAITMLTDAVEKFPES
                     AQLRFYYGTLFDRTGKKDRVITEMKKVLELDPKHVQAMNYLAFTWAEGNTN"
BASE COUNT          440 a          335 c          297 g          494 t
ORIGIN      
        1 attggtattg ccttcagccc aagtgaaagc caagtaattc atcgcttgga catgttttgg
       61 atccaactct aaaacttttt tcatttccgt aataactcga tctttttttc cagtgcgatc
      121 aaagagggtg ccgtaataga atcttaattg cgcactctcc ggaaactttt caacagcatc
      181 agttagcata gtgatggctt tattgtattc accgcgctca tctaataaag aagcatacat
      241 tgcatataat tgagcttgat ctttacgttc tttcagtgcg ctttcaacca cttctacagc
      301 gtcacctaat cgccccttgt ttttcatcat ataagcggta tgaaccatag cttcgccaaa
      361 gaattggctg gcagtaggaa tctttttaaa ttgatagatc gctttttcat aatctttggt
      421 ctcttcataa acagcaccta gataaaaacg tatcttatcc gattctggag catcacgaag
      481 gatatcctct aactttgcgg cggcggcaat aaaatctttt ctttcaatca agatcaaagc
      541 catcttaagt ttaatattta agctatcctc tgtcgtattc tcaagaatct gatattgctc
      601 ataggcaaga tcatattgtt cactttccac atatatttga gccaaaattt cagccacttt
      661 tcgattcggg ccttgttctt tttgaaattt tctataaagc gcgagagctt tctcttcttg
      721 acctttttgg gtataaaaag aagcaatggc cacgtatccg tctaaaaagt cgggtttcaa
      781 ctcaattgcc ttttgataag ctttcataga agctttttga aaaacttctc ctttttgctc
      841 tgagcgcact cggccaatat aataatatgc cagatgagga gttgaataat cttcattctt
      901 aatcaaagat tcaaaaaatt tcacagcctt atcgaactgt ttgatttcag tataagccgc
      961 tcccaaataa acgggggcct ccacattatg gggttgcact tttagtacat attcatattg
     1021 gcctatggct ttcggaaaaa gtttcaaaga taaatagagc ccccctaaga gcaagcgtcc
     1081 ttcgatattt tttgggtctt tgcggatcga ctcttcggct tgggtcaacg attccgtgaa
     1141 ttgtgcggtc ttaaaatact ctgttgccaa gcggatgttc accatgggcg actcttggtc
     1201 ataaagaaga gccattttga aagcatcgat agctttgcta tgatcaccat caagactata
     1261 gctttctccc accgtaaagt aataatccgc ttgagttcta agataaaagg gatctaaaac
     1321 cgttgtcgta ccttcgcctc cactttgcaa atgtggagta aatgccgcag gagccctgtt
     1381 tttatcatta aaagcggcat catagtaagg agccgtatct gaatcctgaa gtgtcgcgca
     1441 agctgagagg atgagtagta gcgctaatac tatgaaggga ggccttgtcg agcgatgaag
     1501 catgaaaact ccttagtcac tcaacttttc ggagccttca acagggcact taaacagaat
     1561 tgatga
//
LOCUS       DATBTE010000100         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1650925, whole genome shotgun sequence.
ACCESSION   DATBTE010000100 DATBTE010000000
VERSION     DATBTE010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650925"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            776..>1732
                     /locus_tag="VIG33_03940"
     CDS             776..>1732
                     /locus_tag="VIG33_03940"
                     /inference="COORDINATES: protein motif:HMM:NF036725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY8270013.1"
                     /translation="MALRLPLVGVRIHLSGSIPDDSTPEQIDGIHSFVQKLASKIFSE
                     GGTIIHGSHPSLLKPLENSARSFVDSGGTKDALTLVRAQKYTETTEQLAEIDFQRQYA
                     VVQIVPAMTGNPNESLIPMREWMAERSDVIICIGGKWWDTDKVRAGVPAELDAMLEQG
                     KPGFIVAGFGGAIKGYIDEDLALLSRLKNGLPDMKNREIAESTSVESLVETIISQLKL
                     LPLVRQSVSRGRNFRILVLDGGGLRGTFTAAVLAKWDDMIKSGGGNELVKHFDLVAGT
                     STGAILAIGLGLGLKPIDILQFYRKQGPKIFPKDRKLRHWLKS"
BASE COUNT          488 a          352 c          429 g          463 t
ORIGIN      
        1 acgaatgcat ccaaccatgg cggaagttaa aatacacagg ggttcatatg cctgacgaca
       61 catatcgata ctctgatgcc agcccgcttg aagggcgcgt atatggctat cgggcgcagt
      121 gccggagaca gatggcgtca ctgaagagca gcggaccgta attcttgatt tcgtccggag
      181 gttttcaagt cgtgtactcg agcgcggcgg ttacataatt catgggagtc atccgagttt
      241 tacactaact ttacttgaat cggcaaagct gcatcaggag caaggaggtc ggaaagactc
      301 actaattctt gcgacttccc gattttggtc gaagaatccg gcgatcgttc ctgtagatga
      361 gtggcgcaaa accgcaatag tttacgagac gccggaagta tctggaaaga atgcacgcga
      421 tgacagtgta gagcttcttc gtaagtggat ggccggccaa agcagacata caatttatcg
      481 cttgaacggg caaaccgccc ctttgtgatt ggccgaaaaa attctccgca gatcctgtac
      541 ctttctcgtt tacaggttta gagtgtcttg atctttactg ttgtgcagat gttgatcagg
      601 cgtatacgaa caggcaagct cttcagatta atcgttcact tggtatgaca tagggcaaag
      661 tcgtcaaata tgatccatgg attaatggaa cgtggccata agtggttcgt cattctagtt
      721 tttgaataat cataatctaa atttattcat ttgatatcgg aaaaaagaga agtttatggc
      781 attacgacta cctttagttg gagtaagaat tcatctctcc ggctccattc ctgacgattc
      841 aactccggaa caaatagacg gtatacactc atttgttcaa aagctcgcta gtaaaatatt
      901 tagtgaaggc ggaacaatta ttcatggtag ccatccttca ttattgaaac ctctggaaaa
      961 ttcagcaaga agttttgttg acagcggtgg aaccaaggat gctcttactt tagtccgagc
     1021 tcaaaagtat accgaaacca ctgaacaact ggcggaaatt gactttcaac gtcaatatgc
     1081 agttgttcaa atcgttcccg ctatgactgg caatccaaat gagagtttaa tacccatgcg
     1141 tgaatggatg gcagaaagat ctgacgttat tatctgcatt ggcggaaaat ggtgggatac
     1201 cgataaggtt cgagcaggag ttcctgccga actcgacgcg atgttggagc aaggaaagcc
     1261 aggatttatc gtggcgggtt ttggaggtgc aatcaaaggg tatattgatg aagatttagc
     1321 tttgctttct cggctcaaga acggccttcc tgatatgaaa aaccgagaaa ttgccgaaag
     1381 tacctctgta gagagtcttg tggaaactat catttcacag ctaaagcttt tacctcttgt
     1441 tcggcaaagc gtgtcaaggg ggcgtaattt ccgaattttg gtccttgatg gtggtggact
     1501 gcggggaacc tttacggcag cagtacttgc taagtgggac gatatgatca aaagcggcgg
     1561 cggtaacgag ttggttaagc attttgacct cgtagcagga acatccactg gagcaattct
     1621 tgccatagga cttggtcttg gtttgaaacc catcgatata ctgcagttct accggaaaca
     1681 aggaccaaag atatttccta aagacagaaa gttgcgacat tggctaaaat cg
//
LOCUS       DATBTE010000101         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2129048, whole genome shotgun sequence.
ACCESSION   DATBTE010000101 DATBTE010000000
VERSION     DATBTE010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2129048"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..880)
                     /locus_tag="VIG33_03945"
     CDS             complement(311..880)
                     /locus_tag="VIG33_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270014.1"
                     /translation="MKLKMKITFFIFLLCACASPNKARDLDTKVDLSAPVAGGGVIGL
                     KDGDMIYQRKVMMNEELRALELDVYSLEAKVYGGPRYLDNRGLYGSLKDCRAELSETK
                     NGGDGKLLWTEKREYVTPDEDLKNIGLEEKKKIIGVSEEFLKDRLERLKSYRTVLMAR
                     QDEYETKIQVCDLELRSKQPNSAKIKPAE"
     gene            1213..1761
                     /locus_tag="VIG33_03950"
     CDS             1213..1761
                     /locus_tag="VIG33_03950"
                     /inference="COORDINATES: protein motif:HMM:NF036842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270015.1"
                     /translation="MISFDKKSDPILDWNQITIEVVRAGGDIGPGFPSRNMAIVHAAI
                     FDTVNAIKPTAESYLYHQEAPRSTNLELAVTGAAYTSLVALYPAQKNLIMEKLLRELS
                     QAKGGPFKNNPNKILSDSSFGFGEKVAAVILASRQNDGSNIEIPYNPPPNPGVWLPTL
                     PDFTPALGTDSFYLPEKFLKMR"
     gene            1898..>2416
                     /locus_tag="VIG33_03955"
     CDS             1898..>2416
                     /locus_tag="VIG33_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270016.1"
                     /translation="MKTSFYSIVFSITLTFGCFPTGAQEEGPLAPNGSSFASELKSNL
                     KQTYEIILNQNSSTLERNQAINLLFENVENICIQHCDIAVKTLSEFNDQFPADEKKSQ
                     MENLIKISTGREVSNQYLDKVIAPLNFAKKLEDVYNKDPEGIKLKIQASIQAVKVIVN
                     FEQVAIFKAMSAD"
BASE COUNT          743 a          521 c          455 g          697 t
ORIGIN      
        1 aaacgtagct aaacatatct tcttgttggt cttgtttata tccgcgcatg cgacattatt
       61 acgaaataat tctaaatatg aaaagaaaaa tcctggaggt ggaaaaacag gcccgaaggg
      121 ccgtttttca ccgacctgtt agtcttaaat tacgatacca agaaaacttt aaaaatgttt
      181 tcttggttca acgccggctt ttacccatca atattgatgt ttgtagccaa tttaagaaga
      241 agaagaagct cagtaaatca aatttagcaa ttttgaagat tttaactata aaataaaggt
      301 ctaaaacgtc ttattccgct ggcttaattt ttgcggaatt aggctgttta gacctcaact
      361 ccaaatcaca aacttggatt tttgtttcat actcatcctg acgtgccata agcacagtac
      421 gataactctt caaacgttca aggcgatcct ttaagaattc ttctgagact ccaatgatct
      481 ttttcttttc ttcaagacct atatttttga gatcttcatc aggcgtaaca tattcgcgtt
      541 tttcggtcca aagcagctta ccgtcccctc cgttttttgt ctcactaagc tcagctcgac
      601 aatcttttaa ggatccatat aggccacgat tgtcaagata acgagggcca ccataaactt
      661 ttgcttcaag gctataaacg tccagctcca gtgctcgaag ttcctcattc atcatcactt
      721 tacgctggta gatcatatcc ccgtccttca atccgatcac tccacctccc gcaaccgggg
      781 cgctaagatc cactttagta tccagatccc gcgccttgtt gggcgatgca caggcacata
      841 gtaaaaaaat aaaaaatgta attttcattt ttaatttcat ttatttctcc gatatctaat
      901 cgtttagctt gtcccagtgg gcttcatggg caagacgtca aattggcagt acgctttgca
      961 ttttaaacaa tcacagctaa gttggcaaat cgaattgatg ctggctaaaa ctaacaagtt
     1021 tgatacccat gatgaagctt aaaaataatc ctaaaaatga tctctgttcg aacagccctg
     1081 ttaaaaaaac agagtattgt ttaactatta ttcgctctgc attaatgagc tgggccttta
     1141 tcggacacct gactcgtcat gatttttgca gctcttccta agtaaagaga gaaatctcaa
     1201 acagggggaa tcatgataag tttcgacaag aagtcagatc cgatccttga ttggaatcag
     1261 atcacgattg aggtggtccg cgctggtggg gatatcggcc caggctttcc ctctagaaac
     1321 atggctatcg ttcacgctgc tatttttgat accgtcaatg cgattaagcc gacggcggag
     1381 tcttatcttt accatcaaga agctccgcgc tcgacgaacc tcgagcttgc cgtgacaggt
     1441 gctgcttata ctagtctggt ggcgctttat ccggctcaaa agaatctaat tatggaaaaa
     1501 ttactgcgag aactttcgca agctaaaggc ggtcctttta agaacaaccc taataaaatc
     1561 ttgagcgatt ccagttttgg ttttggagaa aaagtggcgg ccgtgatttt ggccagtcga
     1621 caaaacgatg gctcgaacat cgaaatccct tacaatccac cgccgaatcc tggagtatgg
     1681 ctgccaactt tgcctgattt caccccagct ttgggaacag actcttttta tctccccgag
     1741 aaatttctca aaatgagatg actgctcaat aagcgaacaa cgcaaggcct attttcggca
     1801 gctacctttt taaaagcctt tagaattgca taaatgatgt tcagacaggc atgacgcttg
     1861 ctcattaaaa tatcaggaga agaggcctgg aggtttcgtg aaaacatctt tttatagtat
     1921 tgtatttagc ataaccctga cctttgggtg ttttccaacg ggagctcaag aggaaggacc
     1981 cttagcccct aatgggtctt cgttcgctag tgagttaaaa agtaatttaa aacaaactta
     2041 tgaaattatt cttaatcaaa actcctctac ccttgaacgg aaccaggcga taaacttact
     2101 tttcgaaaat gtagaaaata tctgtatcca acactgtgac atagcggtaa agactttatc
     2161 agaatttaac gaccagtttc ctgctgacga aaaaaaatct caaatggaaa accttattaa
     2221 aatctcaact gggagagaag taagcaacca atacttagac aaagttattg cgccgctaaa
     2281 cttcgcaaag aagctcgaag acgtttacaa caaagatccc gagggaatta aactaaaaat
     2341 acaagcatca attcaagctg taaaagtaat agtgaatttt gaacaagtag ctatatttaa
     2401 agcaatgtca gcagat
//
LOCUS       DATBTE010000102         4320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1583045, whole genome shotgun sequence.
ACCESSION   DATBTE010000102 DATBTE010000000
VERSION     DATBTE010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583045"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VIG33_03960"
     CDS             <1..235
                     /locus_tag="VIG33_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270017.1"
                     /translation="SVIARTVVDSGGCAFLPIPYILNEMKLEILKVIGPEEGLWKHSL
                     SLISRKQIKYDPLFEEIKNNLIRLNTPFKQINI"
     gene            complement(225..1817)
                     /locus_tag="VIG33_03965"
     CDS             complement(225..1817)
                     /locus_tag="VIG33_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY8270018.1"
                     /translation="MTHQTDVDVLIVGAGPTGLTLACLLQQYGVKFKIIEKNKTITTR
                     SKALAVQARTLELFEQLGIAEKAIEPGHPAEGLDLVVRGKLRATVDLITYGRGLTKYP
                     YLLILEQCKTEQLLWEKLKTQGSEVEWQKELVELQLHADSVTAVIHEFEKLEIIQAKY
                     IVAADGARSAVRTLLRIPFEGETYENRFLLADVALRGNISRHHITLCLSQQGFAGFFP
                     MYGTRRFRAIGILPDDVPENADKEFETIAKKIAEQAQVPMTLSDPLWISTYKLHHRSI
                     PHFKVQRCFFAGDAAHVHSPAGGQGMNTGIQDAFNLAWKISWVLEGRARENLLETYHE
                     ERFPIAKKLLRSTDRVFGFVTGRSAWLRFLRLYILASVLKTLVKSRTFRSFIFRTVSQ
                     IGIHYRQSKLSEQCFCSDSVRKAGDRLCEEVLDGSFQAYVVGGQQAQREATDILRRYF
                     EVRIKIYTLDKKPDIYSVFQTFGIYKDGLILVRPDGYVSYCSDGFNSLALLNYLNRFF
                     LPKDFDLEKEREQPFQTENFIY"
     gene            1907..3136
                     /locus_tag="VIG33_03970"
     CDS             1907..3136
                     /locus_tag="VIG33_03970"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEY8270019.1"
                     /translation="MQKIKVTNPVVELDGDEMTRVIWSFIKNKLITPYLDIDIKYYDL
                     GMEHRDATNDEVTIAAAEAIKKYNVGIKCATITPDEERVKEFKLKQMWKSPNGTIRNI
                     LDGTVFREPIVCKNIPRLVPNWTAPICIGRHAFGDQYRATDFVTKGKGKLTITFEPEN
                     GEKISHEVFNFKGDGVAMAMYNTDESIRGFARSCFNMALTKKWPLYFSSKNTILKKYD
                     GRFKDIFQELYDKEFKNLYKDAGITYEHRLIDDMVASALKWNGNFVWACKNYDGDVQS
                     DTVAQGFGSLGLMTSVLVTPDGKTIEAEAAHGTVTRHYRQHQQGKPTSTNPIASIFAW
                     TGGLAHRGKLDNNQELVNFAHTLEKVCVQTVESGKMTKDLAVCIYGEKTTPDQYLNTE
                     AFLELLDSNLRKSLTEK"
     gene            3201..3590
                     /locus_tag="VIG33_03975"
     CDS             3201..3590
                     /locus_tag="VIG33_03975"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEY8270020.1"
                     /translation="MEHIELLFRWILGLQLAFWGLNGFFHWRPVSPSAKPINDFTEAC
                     IKSKFIMPTVKSFEVIFGIFLVAGFCTPLSLVALAPIIFVISGLHLFHNARPWPVITA
                     ITMPFLALVILNYENWRMFFQFTKYNL"
     gene            complement(3597..>4320)
                     /locus_tag="VIG33_03980"
     CDS             complement(3597..>4320)
                     /locus_tag="VIG33_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8270021.1"
                     /translation="TGLPIVVNVPSSFIAVIEALGSKRADIAIMNTFGYLLAHEKYGA
                     EAVLTGFYKKRGTYCGQIIVRKDGPKTLQELHNKKIAFVDPSSGSGFVLPSKLLKEKN
                     VKPKEVVFAGRHDSVATMVYQRQVDAGATYHIPEESSVPQDARKLVMTQFPDIFDQVV
                     ILAKTDPIPNDPVVIRKGFPPELRQKMIEAFKKMAKDKKGAKILFDLYHMDDLKDANL
                     EDYDKVQKILLDLGKSAQDFVK"
BASE COUNT         1276 a          938 c          866 g         1240 t
ORIGIN      
        1 atcggtcatc gctcgcacgg tcgtggatag tggcggttgt gcttttttac ccattcccta
       61 tatcttaaat gaaatgaagc ttgaaatttt aaaggttata ggacctgagg aaggactctg
      121 gaagcacagt ttgagtttaa tttcgagaaa acaaataaaa tacgacccgc tttttgagga
      181 gatcaaaaac aatttaatca ggctcaacac ccctttcaaa caaatcaata tatgaaattc
      241 tcagtttgga agggttgttc ccgttccttt tccagatcaa aatctttggg taaaaaaaag
      301 cgattgaggt aattgagaag ggccaatgaa ttaaaaccat cagaacaata tgacacatag
      361 ccatcgggac gcactagtat cagtccatct ttataaattc caaaagtttg aaaaacagaa
      421 taaatatcgg gttttttatc caaagtatat attttaattc ttacctcaaa atagcgcctg
      481 agtatatcag tggcctctcg ctgggcttgc tgaccaccca ccacataagc ttggaaggaa
      541 ccatctagaa cctcctcaca gagacgatct cccgccttac gtactgagtc cgaacagaaa
      601 cactgttcgc tcaatttgct ttggcggtaa tgaattccaa tttgagaaac cgttctaaag
      661 atgaaagacc taaaagtcct gcttttaacc agggttttaa gaactgaggc cagaatataa
      721 agtcttaaaa aacgcagcca agcacttctt cccgtcacaa aaccaaagac gcgatctgtg
      781 gagcgcagaa gtttttttgc gatgggaaat ctttcttcat gataagtctc taaaaggttt
      841 tctcgggctc gcccttctaa tacccaagaa attttccagg ctaagttaaa ggcatcctga
      901 atacctgtat tcattccttg tccacctgcg ggactatgaa catgtgccgc atcccccgca
      961 aaaaaacagc gctgcacttt aaaatgaggg atactgcgat gatgcagctt gtaggtggaa
     1021 atccacaggg ggtccgatag ggtcatgggc acttgagcct gctctgcaat ttttttggca
     1081 atagtctcaa attctttgtc ggcattctcc ggcacatcat ccggcaagat gccaatggcg
     1141 cgaaatcgtc tcgtgccata catgggaaaa aatccggcga aaccctgctg tgaaaggcaa
     1201 agagtgatat ggtgtcgtga aatatttcct cggagcgcca catccgccaa taagaagcga
     1261 ttttcatatg tttcaccttc aaaaggtatg cgaagcaaag ttctgactgc gcttctggct
     1321 ccatccgcag ccactatgta tttcgcctga atgatttcca acttttcaaa ttcgtgaatc
     1381 accgctgtta cagaatcagc atgcaattgc aattccacta attctttctg ccattccact
     1441 tcagagccct gagtcttgag tttttcccat aataattgct cggttttgca ttgctctaaa
     1501 atcaatagat aaggatactt ggtaagacct cttccataag tgataaggtc tacggtggcg
     1561 cgcaattttc cccgtaccac caaatccaag ccctctgcgg gatgccctgg ctctatagct
     1621 ttttcggcaa ttcccaattg ctcaaaaagt tccaacgtcc tcgcttgaac cgctaaagcc
     1681 ttggatcttg ttgtaatagt tttatttttt tcgatgattt taaacttcac accgtactgt
     1741 tgcaaaaggc aggcgagagt tagacctgtt ggcccggcgc caacgatcaa aacgtcaaca
     1801 tcggtttgat gtgtcataga tggctcccga cgcaaaagca tatcgacttt caaaagtgaa
     1861 ccacttataa tagcaaaaaa acaattacct tgaatggaag atttgcatgc aaaaaattaa
     1921 agtcaccaat cctgtagtgg agctcgatgg cgatgaaatg actcgcgtaa tctggtcctt
     1981 tatcaaaaat aaacttatta ctccctatct tgatatcgat atcaaatatt acgatttagg
     2041 catggaacat cgtgacgcca caaatgatga agtcacgata gctgcggcgg aagccattaa
     2101 aaaatataac gtgggtatta aatgcgcgac gatcactcct gatgaagagc gcgtaaaaga
     2161 atttaaattg aaacaaatgt ggaaatcacc caatggtacg attagaaaca ttttagatgg
     2221 caccgttttc cgcgaaccta tcgtgtgtaa aaacattcct cgcttagtgc caaactggac
     2281 cgctccgatt tgtatcggcc gtcatgcctt cggcgatcaa tatcgcgcca ccgattttgt
     2341 aaccaagggt aaaggcaaac tgactattac ttttgagccg gaaaacggtg aaaaaatttc
     2401 ccatgaagtt ttcaacttca aaggcgatgg cgtggccatg gcaatgtaca atactgacga
     2461 atctattcgc ggttttgccc gttcttgctt caatatggcg ttgactaaga agtggccttt
     2521 gtacttttct tcaaaaaata caattctgaa aaaatatgac ggcagattta aggatatttt
     2581 ccaagagctt tacgataaag aatttaaaaa tctctacaaa gatgctggca tcacttatga
     2641 acatcgcttg attgatgaca tggttgcctc ggccctaaaa tggaatggca attttgtatg
     2701 ggcttgtaag aactacgatg gagacgttca atcagacacc gtagcacaag gctttggatc
     2761 attaggtttg atgacttcag tgctagttac cccagatggt aaaaccatag aagccgaggc
     2821 agctcacggg acagtgactc gtcactatcg tcagcatcaa caaggcaaac caacctctac
     2881 caatcctatt gcatccatct ttgcatggac cggtggctta gcccatcgtg ggaagttgga
     2941 taataaccaa gagctcgtga atttcgctca tactttagaa aaggtctgcg ttcaaacagt
     3001 agagtctgga aagatgacaa aagacttagc ggtatgtatt tacggagaga aaacaactcc
     3061 agatcaatac ttgaatacag aagctttctt ggaactttta gatagcaacc taagaaaatc
     3121 ccttacggaa aaataaattt ccagcatgca cagcttaaat cttgtatttg gcaggattta
     3181 agctgtacct ataaataatg atggaacata tcgaattatt attcagatgg attttggggc
     3241 tacaactggc cttttgggga ttgaacggtt tttttcactg gagacccgtc tccccttcag
     3301 caaagcctat taatgatttt actgaggctt gcataaaaag taaattcatc atgccaacgg
     3361 taaagagttt tgaagttatt ttcggaatct ttttagtggc aggattttgt acgccacttt
     3421 ccttagtggc cctcgctccg attatatttg tgatcagtgg tttgcattta tttcataacg
     3481 caaggccatg gccagtgatt accgccatta caatgccttt tttagctctg gttattttga
     3541 attatgaaaa ttggcgaatg ttttttcaat tcacaaaata caatttataa ctaattttat
     3601 ttaacaaagt cttgagcgga ttttcccaga tctaataaaa ttttctgaac tttatcgtag
     3661 tcttctaaat tggcatcttt tagatcatcc atgtgataga gatcgaaaag aattttagct
     3721 ccttttttgt ctttcgccat tttcttaaaa gcttcaatca tcttctggcg aagttcaggc
     3781 ggaaaaccct tgcgaataac tacaggatca ttcggaatag gatccgtctt tgcaagaatc
     3841 actacctggt caaagatatc agggaattgg gtcatcacca gtttgcgcgc atcctgcgga
     3901 acgctacttt cctcaggaat gtgataagtg gcacccgcat ccacctgtct ctgatagacc
     3961 atggttgcca cgctgtcatg acggccagca aacaccacct ctttgggttt tacattcttt
     4021 tctttcagaa gctttgaagg cagcacaaag cccgaacctg aggaggggtc tacaaatgct
     4081 attttcttgt tatggagttc ctgcaaagtt ttcggcccat cttttcttac tatgatctgg
     4141 ccacagtagg tacctctctt cttatagaac cctgtcagta ccgcttcagc accatatttt
     4201 tcatgggcta agagataacc aaaagtattc ataatagcga tatccgcacg tttagatccc
     4261 aatgcttcaa tcaccgcaat aaaacttgat gggacgttga caacaatggg aagtcctgtt
//
LOCUS       DATBTE010000103         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2130052, whole genome shotgun sequence.
ACCESSION   DATBTE010000103 DATBTE010000000
VERSION     DATBTE010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2130052"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..>1555
                     /locus_tag="VIG33_03985"
     CDS             30..>1555
                     /locus_tag="VIG33_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270022.1"
                     /translation="MRDAGTVTPWSTIVPVTMAEEPTYVPEEDRERIVAYQKYNSLYW
                     SEDRAVEIVRDAEDGTPLYVPRPKMIVDTTAHYLLKGLTIDLEDPESQSELNRFLQAF
                     FDREAFLARFNIAKLKGVALGDWIFHITADPLAPPERRITLNTVDPAAYFPEYHPDDQ
                     ETRVGVRLMEPWVDPADTSKTLVKVLYYFFDWQNPADRRVWREEAIWEVEDWWNQAKA
                     KKYKNLLPLEPLDERITEIPVYHFRNAEWDGFLFGNSELKGVEKIFRGIDQAISDEEI
                     ALALVGLGVYATDAGRPTVNGREVDWVVSPGTVLEVPGATMIKRLEGITSVTPVQDHL
                     RYLDSALKEATGTSDIALGVIDAQTAESGIALAIKFIPTLAKIEYRDQSGLAKLTQMW
                     YDLTSWFRVFESVDFGDTLILPRIGEKLPVNHVQVFNELNNLFDRKIISAQFYRDECE
                     RRLGYNFPENIAKQILDEQIAQLEIMQRQMQIQASVAPPDSNGDQPNQSNNKNRVNES
                     "
BASE COUNT          400 a          325 c          395 g          435 t
ORIGIN      
        1 caacgtaagg caaagtttag gagagttgca tgagagacgc cgggacggtg acaccatgga
       61 gcactatcgt tccggtgacc atggccgagg agcctaccta cgttcctgaa gaggatcgtg
      121 agcggatagt tgcttatcag aagtacaata gtctttactg gtctgaggac agggcggtag
      181 agattgttag ggatgcagag gatggaacac ctctctacgt tcctcgtcct aagatgatcg
      241 tggatactac tgctcactat cttcttaagg gattgacgat tgatctggaa gatccagaaa
      301 gccaaagcga gttaaatagg tttctccaag ccttctttga tagagaagct ttcttagcta
      361 ggtttaatat tgccaagctt aagggagtag ctcttggaga ttggattttc cacattacgg
      421 ctgatccctt ggctccccct gagcgacgta taaccttaaa caccgtcgat ccggcagctt
      481 atttccccga gtatcatccg gatgatcagg aaactcgagt cggggttcgc ttgatggagc
      541 cctgggtcga tccagctgat accagtaaaa ctttggttaa ggtcctgtat tatttcttcg
      601 actggcaaaa tcctgctgat cgtcgcgtgt ggcgagaaga ggctatttgg gaagttgaag
      661 attggtggaa tcaggctaag gctaagaaat ataagaatct tttacctctc gagcctcttg
      721 acgaacgtat tactgagatt ccagtctatc acttcagaaa tgctgagtgg gatgggttcc
      781 tttttggtaa cagtgaatta aagggtgttg agaaaatttt ccgtggtatt gaccaggcta
      841 tttccgatga ggagattgct cttgccctgg ttgggcttgg tgtctatgcc actgatgctg
      901 gtcgacctac tgttaatgga cgggaggtag attgggtagt ttctccagga actgttctcg
      961 aagtcccggg cgccactatg atcaagcgct tagagggaat cacctcagtt acgcctgtgc
     1021 aagaccactt gcgatatcta gacagtgctt tgaaagaagc tactggtact tcagatattg
     1081 ctttgggcgt gattgacgct cagactgctg agtctggtat tgccctcgcc attaagttta
     1141 ttcccacact tgccaagatt gaatacaggg atcagtctgg gttggctaag cttactcaga
     1201 tgtggtatga ccttacttca tggttccgag tatttgaaag tgttgacttc ggtgatacac
     1261 ttattcttcc tcggattggt gagaaacttc cagttaacca tgttcaggtc ttcaatgagt
     1321 taaataacct cttcgatcgt aagattatca gcgctcagtt ctatagggat gagtgcgagc
     1381 gaaggttggg ttataacttc cctgaaaaca ttgctaagca aatcttggat gaacagattg
     1441 cccagcttga gattatgcaa cgtcagatgc agattcaagc ttcagttgct cccccagata
     1501 gcaatggcga tcaacctaac caatcaaata acaaaaacag agtcaacgag tccaa
//
LOCUS       DATBTE010000104         7147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_969323, whole genome shotgun sequence.
ACCESSION   DATBTE010000104 DATBTE010000000
VERSION     DATBTE010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969323"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VIG33_03990"
     CDS             <1..650
                     /locus_tag="VIG33_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY8270023.1"
                     /translation="DFRKHLQPSYRETFARLALGQTPDALYIGCSDSRVAVNVFASTD
                     PGDLFVIRNVGNLVPPCGKDGHSTADESEAAAIEFALGTLNVSNIIICGHSECGAMQA
                     LAGGREKVKDKNLRSWLRHGEKGLEDLTLRRDFAKTLSKHNRLSQFNVLRQIEHLKTY
                     PIIQERLNLGTLHLHAWWFELNQADVYAYEHSQNQFVLFDEATGERILAGLEKDA"
     gene            complement(647..1828)
                     /locus_tag="VIG33_03995"
     CDS             complement(647..1828)
                     /locus_tag="VIG33_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270024.1"
                     /translation="MNCAALLFALAICFTLPSSKAWSQENSSQLQQVQLPAAHEQQNH
                     DSLVISVSDKISYVIESSPDVSKMKEALGLTIPSHVREHILHKGGVIPPEVNPLAGYE
                     TLSAEKKEQFKKTRIMFLKNVARALNATKFAFGAGSIIGNTLSFVKDSVVTLFKKNVS
                     SETSAPKDKVTFKENSHNAVQATLQALDYKLWNQAPLLLESNEFGVSASLGILIENGV
                     QYNGMGGSEELGLSFGYNKTDKAFVFEIFHNSEKFSYTPAVISIAAIVVRAGLTVFQK
                     ENTQPVTVKTGNSFYPPVVPVFKSEGTDNFSTGFSTSLGFPPNPLVDLLTYSNKYTRN
                     TIVRITVSPLMKGYVRLYIGDIVGSVKIVTFRMMEIFKWVSQRVTLYSKGGRSCEGAF
                     L"
     gene            complement(2029..2280)
                     /locus_tag="VIG33_04000"
     CDS             complement(2029..2280)
                     /locus_tag="VIG33_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270025.1"
                     /translation="MSILRSDTGNNPEKERSVGGLIYLIITAAVFIAIIIFWVMGANK
                     NLNPKKSGDTTKSAPDKTPQTGASPSTLSPASGDQNVNP"
     gene            2571..2894
                     /locus_tag="VIG33_04005"
     CDS             2571..2894
                     /locus_tag="VIG33_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270026.1"
                     /translation="MMEARKYNIPRPDLEGGADIKYWSSINTPVLAENGEVEYIIRRV
                     EDQTTAVHQEGERKTLEQERDLSLLPPSIFWQLSARMDISNASILLLNAFLDILKRNS
                     TLNQW"
     gene            3386..5089
                     /locus_tag="VIG33_04010"
     CDS             3386..5089
                     /locus_tag="VIG33_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270027.1"
                     /translation="MKIFFKYCYGLLRVIFCVGFGISLAGCDKNIDANSNEGSEILLS
                     TDADSSFSISKKIGQSCSTQTFQGSSTQEKPVDVLFVVETSPSMVDIRPKVLQGINGF
                     IAGLPLNSDFNIAVMLSHGSTSHLSGKLYKTAMEPLVLQSSQLTNEQIQTYLTAKLAG
                     MPFDADSGGGEEGMFSLFRGITTPSLLEESRAASFFRADAALSVVVIADRRDICASVP
                     EGVPAETDPVKIDARVRDCEGLTAAGLVNRLTLLKGSQPLHITGIIYTDAPAPEGKEI
                     SYGYTDLISLKSGVAIDIARDDISAGMAPIIALGGQSSQKVFTLTQADADPSTIKVTV
                     NGQEAPFTFDGTKVTLTGAISTGATIVITACSKGENPYVGNCRVFEKADQWDHWTPIA
                     STQDLELKDKSGNFSVGAVRNLVIKNVSGALKVQSAINIPSISFLSGNSYIRLAGDIT
                     TISDSASVRIDKAGNVGTVLNTRGGIQLNALTIGSYIKAAGNSCIRARSIGKVQSMNG
                     TKTIIANEINELSAVTGVVHVYGAVIKSVIDTGGRVCLHNGAKVVNSSNVSGVFAKCE
                     Q"
     gene            5197..5589
                     /locus_tag="VIG33_04015"
     CDS             5197..5589
                     /locus_tag="VIG33_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEY8270028.1"
                     /translation="MKLKLPLIARILLGLIFFIFGLAGLLNLIPVPPDLPERLVTFNT
                     GLAASGYFMTLLKATETICGLLLLMGSFVPLALVVLAPIVINIILVHAFMAPSGLPLA
                     IVIGLLLFYLSFFAQPYSGVIKQLFRRK"
     gene            complement(5611..7032)
                     /locus_tag="VIG33_04020"
     CDS             complement(5611..7032)
                     /locus_tag="VIG33_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270029.1"
                     /translation="MVHGTGKPVELAPFKTGASTLLAFSTIFSIASCTSKSEQNGSAG
                     TFKIIGKVANTSDFSDVTVQITSVQSGLTSKTLILTAAEERPNSSGELLIGVEIGCYN
                     MSVIKPGFRSRGFENVCVNDSTPEYDLGQIGLSVERRFVMTASLDKYVFMAGGYNSDS
                     MYSTVDVLDVTTGKMDVANLSKRRARGVGVAVGNKAIFAGGEACESEGYSSCAISSIV
                     DIFDISSKTWSATSLSTARLDLAAGVTSDGYAVFAGGSTATGASNVVDIFNSNTGAWS
                     TATLPAARFGMSAIAVGTKIFFAGGSGPDSSGVTKTTRIDVFEPSTGIWTVENLSVAR
                     RYFPTVKVGTKLLFAGGQLDCWPCSASGVVDIYDISNGSWSTATLSTPRELLVGASDG
                     VIAVFAGGEANGSQGTDKVDIYDSSTDTWSTSVLSQARTNIAVAVNGNKAYFVGGNLD
                     SQGFSNVIDVFDFASKSFVSSSF"
BASE COUNT         1952 a         1532 c         1598 g         2065 t
ORIGIN      
        1 tggattttag aaaacatctt caacccagtt accgcgaaac ttttgcccga cttgctttgg
       61 gtcagactcc tgatgccctc tatattgggt gttctgacag tcgagtagcg gtaaatgttt
      121 ttgcttcgac ggatcccgga gatctttttg tcattcgaaa cgtaggcaat ttagttccgc
      181 cttgtggaaa agatggtcac tcgacagctg atgagtcaga agccgcggcg attgagtttg
      241 cgctgggaac tttgaatgtg tctaacatca tcatctgcgg gcactctgag tgtggggcta
      301 tgcaagctct tgctggggga agagaaaaag ttaaagacaa aaatcttcgc tcctggctgc
      361 ggcatggtga gaaaggattg gaggatctga ctttacggag ggattttgca aaaacacttt
      421 caaagcacaa tagactttcc cagttcaatg ttcttcggca aattgagcat ctcaaaacat
      481 atcccattat acaggagcgc ctcaatctag gcacactcca tttgcatgcc tggtggtttg
      541 aacttaacca agcggatgtc tatgcttatg aacactctca aaatcaattt gtgttatttg
      601 acgaagcgac aggagaacga attctcgctg gtctcgagaa ggatgcttaa agaaatgcgc
      661 cttcacagga tcggccacct tttgaatata gggtcacgcg ttgggaaacc catttaaata
      721 tttccatcat tctaaaagtg actatcttca cagagccgac aatatcgcca atataaaggc
      781 gaacatatcc tttcatgagg ggggataccg taatgcgaac aattgtattt cgagtatatt
      841 tatttgaata agtgagcaag tccacaagag gattaggcgg aaaccctagt gaagtactaa
      901 atcctgtact gaaattatct gttccttctg atttaaagac aggcactacg ggaggataaa
      961 atgaattccc cgttttgaca gttactggct gggtattctc tttttgaaag acagtgagac
     1021 ccgctctcac tacgatagcg gctatggaaa tgacagcagg cgtgtaagaa aatttttccg
     1081 aattatgaaa gatttcaaat acgaaagctt tatctgtctt gttgtagcca aaggaaagcc
     1141 ctagttcctc agatccgccc atcccattat attgaactcc attttcaatt aaaatcccca
     1201 atgaagctga aactccaaat tcatttgatt ccagcaaaag tggagcttga ttccagagtt
     1261 tatagtctaa ggcctgtaac gtggcttgta cggcattatg tgagttttct ttgaaggtca
     1321 ctttgtcttt aggtgcagaa gtttccgaag atacgttttt cttaaacagc gttactacgg
     1381 aatccttcac aaaactcaaa gtattcccta tgatgcttcc ggctccaaaa gcaaatttcg
     1441 tggcattaag agcgcgcgcg acattcttta aaaacattat tcttgttttt ttgaattgtt
     1501 ctttcttttc ggcggagagt gtctcatagc ctgctaaagg attcacttca gggggaataa
     1561 cgccgccttt atgaagaata tgctctctaa catgacttgg aattgtcagt cccaaggctt
     1621 ctttcatctt actcacgtct ggggagcttt caattacgta gctaatttta tccgaaacac
     1681 taatgaccaa tgagtcgtga ttttgctgct catgagccgc aggaagttgc acttgttgaa
     1741 gttggctgga attctcttga gaccatgctt tcgaacttgg taaggtaaaa cagatcgcta
     1801 atgcgaataa tagtgccgca cagttcatac tcagtaactc ccattttcat tgtccaacct
     1861 acgtccctat gcttgggggc ttatttgatt cgcagctgta aatgcaattt tcatgaccgg
     1921 atttaatgca tttaattgga tttgagcgca ctcgcaaaag aaaatgacaa aatttgttaa
     1981 acaatgttca tatcttttac agtgagggtg gacagctctt cagtctgttt agggatttac
     2041 gttttggtca ccgctcgcgg gtgaaagggt gcttgggctt gctccggtct gtggagtttt
     2101 atcgggagca cttttggtcg tgtccccgga ttttttaggg ttgagatttt tatttgctcc
     2161 catgacccag aaaatgatta tagcgatgaa aacggcggcc gtgatgataa gataaataag
     2221 tccccctacg cttctttcct tttctggatt gtttcctgta tctgaacgta agatagacat
     2281 attaatctcc taaattaagt attgagctat taatatccct agctttggat gctgagattc
     2341 atctttaacg atttcacttt gccaatccca taaacaatga gtgctttgaa taaataagtc
     2401 tggcatagtt ctattctgat ggggtggtat acaactcaca aaggaaacta ttctcgatcc
     2461 taattttcgg aaattttttc gaagtatttc cggataattc aagtgatcga aagggtttga
     2521 gcgctttacg gatttcgttg gaaaaagtgt tacgagaacg cgcaccagat atgatggagg
     2581 caaggaaata taatataccc cgtcctgacc ttgagggtgg ggccgatatt aaatactgga
     2641 gctccatcaa tacgcctgtg ttggcggaaa atggagaagt tgaatacatt attcgccgcg
     2701 tggaggacca gacgacggcc gttcatcaag agggggaaag aaagacactc gagcaggagc
     2761 gtgatctttc tttacttcct ccttcgatct tttggcaatt gtcggcacgg atggatattt
     2821 caaacgcctc aattcttctt ttgaacgctt tcttggatat tctaaagagg aactctacgc
     2881 tgaaccagtg gtgaatttcc ttcacttctg gttgaagacg acgaacatct cagagtgggg
     2941 tttgctatga tgcttgaaag aaggggatat aacgttctgg ttgcagcgga tggcatatag
     3001 gcattgtctt tttgcgagca atacaatggc gatattcacc ttttactgac agatatggtg
     3061 atgccggggc tcaacggctt tgagcttgcc aagagagcta tgcttatccg agacgatgtc
     3121 cgcgtcatcg ttccaatagc tgagtttagc ttaataaata aatcaataat ttcaaaaata
     3181 aatatttatt atttatttaa ggcgatgtca ctttttttgt cgaaaataat aactcggaaa
     3241 aaagctaaag gttttcctct aaaagatcga tcaatagtct aggtatagct gtgacacagt
     3301 tggataatcc caagctactt cagagacgtt tattcaggca attgcaatat ccagcattga
     3361 aataattaaa tgaggagaaa aatttatgaa aatatttttt aagtattgct atggtttgct
     3421 tcgagtgatt ttctgtgtcg gttttggcat aagccttgcc ggttgtgata agaatataga
     3481 tgcgaattcg aatgaaggtt ccgagatcct attgtccact gatgcggatt catcattctc
     3541 gatttccaaa aaaatcgggc aaagttgttc aacacaaact tttcaaggat cttctaccca
     3601 ggagaagccg gtggacgttc tctttgtggt ggagacttcg ccgtcaatgg tagatatcag
     3661 accaaaagtc cttcagggca tcaacggctt tatcgctggg cttccgctca attcagattt
     3721 caatatcgcg gtcatgcttt ctcatggaag cacgtcccat ctttccggga aattatataa
     3781 gaccgcaatg gagcctctgg tgttgcagtc ttcccagctg accaatgagc aaattcaaac
     3841 gtatctgact gcaaagttgg cggggatgcc gtttgatgcg gactctgggg gtggcgagga
     3901 aggaatgttt tctctcttcc gtgggattac aacgccttcc ttgctggaag aatctcgagc
     3961 tgcctctttt ttccgtgctg atgctgcgtt aagtgtagtt gtcattgcgg atcgccgtga
     4021 tatctgtgcc tctgttcccg agggagttcc cgcagaaaca gatccggtga aaatcgacgc
     4081 tcgcgtacgt gactgcgaag ggttgacggc tgcaggactg gtcaatcgcc tgaccctcct
     4141 caaaggatca cagcctctcc atattactgg gatcatttac accgatgcgc cggctccaga
     4201 gggaaaagag atcagttatg gttatacaga tctgatcagt cttaaaagcg gagttgcaat
     4261 cgatattgct cgcgatgata ttagcgcggg tatggcgccg atcatcgctc tcgggggaca
     4321 gtctagccaa aaggtattca ctttgactca agcagatgca gatccttcga ctataaaagt
     4381 gacagttaac ggtcaagaag ctccattcac tttcgatggc accaaggtca cccttacagg
     4441 agctatctct acaggtgcga caattgtaat taccgcttgt tcaaaaggcg agaatccgta
     4501 tgtagggaat tgtagagtct ttgaaaaagc cgatcaatgg gaccattgga cgccgatcgc
     4561 ttccactcaa gatttagaac tcaaagataa atctggcaat ttttcagtgg gtgcggtccg
     4621 caatttagtg attaaaaatg tatcgggagc actgaaggtt cagtcggcga taaacattcc
     4681 ttcaatctcg ttcctttctg gaaacagcta tatccggctt gcgggcgata tcaccactat
     4741 tagcgattca gcatctgtgc gtatcgacaa agctggcaat gtgggtactg ttttgaacac
     4801 gagagggggc attcaattga atgccctgac tattggttct tatatcaagg ctgctggcaa
     4861 ctcctgcatt cgagcgcgaa gcataggtaa agttcaatct atgaacggaa ctaaaaccat
     4921 catagccaac gaaattaatg aattgtcggc agtaacaggt gtagttcacg tctatggtgc
     4981 agtgattaaa tccgtcatcg acactggtgg aagagtttgc ctgcacaacg gcgccaaggt
     5041 tgtgaattca agcaatgttt caggagtctt cgctaaatgt gaacaatagg ggtgatgaga
     5101 ccgactccgg aaactgggtc ggttttttat cttattaaat agagtcttgt aggatttaaa
     5161 aaaataggat gaaataaaga aaaggagact tccactatga aattaaaatt gccattgatc
     5221 gctcgtattt tattgggact tatttttttt attttcggct tagccggtct tttgaatttg
     5281 atacccgtcc caccggattt acccgagcgc ttggtcactt ttaacacagg gcttgcggcc
     5341 agtggatatt ttatgacact tctgaaggcg acggaaacga tttgtggatt gcttcttctc
     5401 atgggatctt ttgtgccatt ggcactcgtc gtgctcgcgc caatagttat caatatcatt
     5461 cttgttcatg ctttcatggc acccagtggt ttgccgctgg cgatagtcat cggattactt
     5521 ctgttttatc tgtcgttttt cgcgcaacct tattcggggg ttattaaaca actcttccga
     5581 cgcaagtgag ggggctctac gttcttaata ttaaaaacta gaacttacaa aagacttcga
     5641 ggcaaaatca aaaacatcaa ttacatttga aaagccctgt gagtcgagat taccacctac
     5701 aaaataggct ttgtttccat ttactgccac ggcaatatta gttctcgctt gggaaagaac
     5761 actcgttgac cacgtgtccg ttgatgaatc gtagatatcg accttatccg ttccctgact
     5821 cccattcgcc tcgccgccgg caaataccgc aatcacgccg tcactcgccc caactaaaag
     5881 ctcgcgtggg gtggaaagcg tggccgtaga ccacgagcca tttgatatat cataaatatc
     5941 cactacgcca gatgcggagc atggccaaca gtctaactgc cctcccgcaa aaagaagttt
     6001 tgtcccaact tttactgtgg ggaagtacct tcgcgcaaca gagagatttt ctacggtcca
     6061 gattcctgta ctaggttcaa aaacgtctat tcttgtggtt ttcgttactc cgctgctatc
     6121 tggtcctgac ccaccggcaa aaaaaatctt agttccaact gcaattgctg acattccgaa
     6181 tcgcgcggct ggtagagttg ccgtggacca agccccagta ttggaattaa agatatcgac
     6241 aacattagaa gcacctgtgg ctgtggatcc cccggcaaat actgcatatc cgtcagacgt
     6301 cacaccggca gccaaatcta accttgctgt ggacaaagaa gttgctgacc aagttttgga
     6361 actgatatca aaaatatcaa ctatagaaga aatcgcgcag gaagaatatc cctctgattc
     6421 gcaagcttct cctccagcaa aaatcgcttt atttccgacg gctactccga cacctctcgc
     6481 gcgtcgctta gacaaatttg ctacatccat ttttccagtt gtgacatcta gaacatccac
     6541 cgtggagtac atggaatcgc tgttgtagcc cccagccatg aaaacatact tatcaagact
     6601 tgcagtcata acaaagcgac gttcaaccga aagtccaatc tgtccaagat cgtactcggg
     6661 agtggagtca tttacacaaa cgttttcaaa tccacgactt ctaaaacccg gcttaataac
     6721 ggacatgttg taacagccaa tctcaactcc aattagcaac tcaccactcg agttcgggcg
     6781 ttcttctgct gcagttagaa tcaaagtttt ggatgtaagt ccggattgca cacttgttat
     6841 ttgaaccgtg acatcactaa aatcagaggt atttgcgact ttgccaataa tcttgaaggt
     6901 gccagcacta ccattttgct cactttttga tgtgcaactt gcaatagaaa atatcgtgct
     6961 gaaagccaag agtgtggaag caccggtttt aaaaggggcc aattccaccg gtttaccggt
     7021 cccatggacc aatgcctaac gccatagcaa aagggaccgg attcaatgga aattgatttc
     7081 cgtcgtaaag gtttccttcg attttatttt caaaagcatt acaaattgtc gaaagttcga
     7141 ttggaat
//
LOCUS       DATBTE010000105         5529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1925280, whole genome shotgun sequence.
ACCESSION   DATBTE010000105 DATBTE010000000
VERSION     DATBTE010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925280"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..338)
                     /locus_tag="VIG33_04025"
     CDS             complement(33..338)
                     /locus_tag="VIG33_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270030.1"
                     /translation="MKKKILALTLLTGVYSVQSWAESQTWVFTSTIPNKKEMLSIPKK
                     ETCRLFTAKLMSNKCVEYKQGYVCPQEDSGQFVISKYSSKSECTKSLNKARRLLARR"
     gene            741..2396
                     /locus_tag="VIG33_04030"
     CDS             741..2396
                     /locus_tag="VIG33_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8270031.1"
                     /translation="MKLQVFAVFISLVISSGAFAGQRYLVVMKSAQSFKTANVALSHK
                     SNFGFRGFGFGTSLREFEKVDAKVVDHLANLNTLVVNAEDSEIAKLKNNSEVAMVEKE
                     VFHPAPKPMKGFISFNKNSKLTSFATSNSVLPQPWGIAAVKAPQAWQASSNKGQGARV
                     LVLDTGIDAEHPSLKANFEKGRDFTGLSDGSDFSDKVGHGTHCSGTIAGVEEASGFSG
                     VAPEAKLLMGRVCSDQGCSNVAIAQGVNWGIEEKVDVISMSLGGMWSTPAEKTAIAAA
                     DAAGVTVVAASGNSGTAVVGYPAALPTVIAVGAINVKLEKADFSQYGPELAVVAPGVD
                     VVSSVPQGTGRESEVIITMNNSPQRVVSTTFQGAKELDVQNDVELVDAGLAKPEDFAG
                     KDFTGKYALISRGEITFGEKVNNALKANAAGAIIYNNAPGLIHGSLTQDGSTLNIAVA
                     MIEQTVGQALKDELLASRPVLASISTVKSDYAHFDGTSMATPHVAGVAALVKAANKSL
                     TPAQVKEVFKKTAQVLPGTNTKNELGSGLIDAQAAVSAALGQY"
     gene            2531..4780
                     /locus_tag="VIG33_04035"
     CDS             2531..4780
                     /locus_tag="VIG33_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY8270032.1"
                     /translation="MTTHLPQIIQDLALILVVAATVSLTFKKMKQPVVLGYLVAGMLV
                     GPHQDWFPTVSDFEGINVWADIGVIFLLFAIGLEFSFRKLLSVGASAGITTAFQVSVM
                     LLLGFVAGQSMGWSWMSSLFFGATISISSTTIVVKTFDELDLKGRRFVSLVISILIFE
                     DLVAVLLMVFLATLAMSREFQGIQLLEQIARLVFFLSLWFMVGVFVLPNLVKRIRLLL
                     NAEATLIISLGLCFLMVVLASKAGFSPALGAFVMGSLLAETSEGERIERVVTSVKDLF
                     SAIFFVSVGMLIDPRVLMEHWASVLLLSLMLIFGKVFSVTCGSLISGQNLKTSLQAGL
                     SLAQIGEFSYIIAGLGMTLKVTNELLYPLVVTMSGLTTFATPYMVRGADGIYSWIEKI
                     VPIHMRKALDTYSLLPAGGGGLATRREYLKNFGLKMLLNTVVIVAIFLAFSEGLLPWA
                     QQKIVNKGMTLIICLTLSLMVSAPFFWALIFSEVKGYAFSLIWQTRAVRTSLLFLSLF
                     RSVWGVILLSVLLTQFLPQFLPMVVILLALSIFILIFYNRLERLYGAIEGRFLSNLYR
                     KSQEEKEAQEKELANAEMSPLAPWDAHLVEFVIPQEAKYVGFPLESLLLREKYGVTIA
                     LIRRGGHRITAPGRQEMLMPLDHIFVIGTDGQLLKFKHFLDFERISEDIAGSGRDYTL
                     ERYLVSEKSPFVGKSIRASGLREATNGIVVGMERGGQRILNPDSGIIIEVGDLLWIAG
                     DRKLIKGLI"
     gene            4799..5005
                     /locus_tag="VIG33_04040"
     CDS             4799..5005
                     /locus_tag="VIG33_04040"
                     /inference="COORDINATES: protein motif:HMM:NF016028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HEY8270033.1"
                     /translation="MTEERFIEIETKITHQEFLIEQLNKVIYEQQGKINQLETILTAL
                     VKRTQEIMNPEPEIGPANDKPPHY"
     gene            5055..5339
                     /locus_tag="VIG33_04045"
     CDS             5055..5339
                     /locus_tag="VIG33_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270034.1"
                     /translation="MAMKNSGKILILIFLFAGLVSCQNSIPTVVNLGSLPKLSSPANS
                     KNFSQLSAASQKEVLASGYKGTLRISQPLGRLQATTSDGHKLQGTVTLQQ"
     gene            5350..5529
                     /locus_tag="VIG33_04050"
                     /pseudo
     CDS             5350..5529
                     /locus_tag="VIG33_04050"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
BASE COUNT         1414 a         1083 c         1328 g         1704 t
ORIGIN      
        1 cgttttgcct ttctaagaac tgccaaaaaa acttatctcc ttgcaagcaa gcgacgggct
       61 ttattcaaag attttgtgca ttcacttttg ctcgaatatt tagaaatcac aaattgcccg
      121 gagtcctctt gaggacaaac atagccttgc ttatactcta cacacttatt agacataagt
      181 tttgcagtga agaggcggca agtttctttt ttaggaatac tcagcatttc ttttttatta
      241 ggaatggtgg aggtaaaaac ccacgtttgg ctttcagccc atgattgaac ggagtaaacg
      301 cctgtgagga gagtgagtgc taggattttt ttcttcatag cgattagtta taagggggga
      361 gtcagagaaa acccactgtt agtattctga cagggatttt tttcatttgc gagaataggg
      421 agtgccgtga aacttgcgga aaccaggggc ttactgttct tgcgtcgcgt tgtttatatc
      481 tgtttaaaat gtatgcaaat acctctatga aaaataatgg aagatattaa attttcttat
      541 attcaatgtt caaatagttc tttattagac atgggttcca aaatggaacc tcctcgggaa
      601 tatcagatca tttcattttt gtaagtgatg aaattgttac ggaaattacc aaaacatttc
      661 ttgcatctgg tgccaatttg gaatctgatt cgcaatagaa ggatttcaaa ttagaaatcc
      721 atggaatttt tggggggatc atgaaattac aagtattcgc agtgttcatt tctcttgtta
      781 tttcgagtgg agcattcgca ggtcaacggt acctagtagt aatgaaatcg gctcagtcat
      841 ttaaaacagc taatgtggct ctatctcata aatcaaattt cggatttaga ggttttggtt
      901 ttggtaccag cctcagggaa tttgagaaag tagacgccaa agttgttgat cacttagcaa
      961 acctgaatac tttggtagtc aacgctgaag attcagaaat agcaaaattg aaaaataatt
     1021 ccgaagtcgc catggtggaa aaagaagttt tccatccagc ccccaagcct atgaaaggtt
     1081 ttatttcttt taataaaaat tcaaaattaa cctcttttgc aacttcaaat tctgttttgc
     1141 ctcaaccttg ggggattgcg gcagtcaagg caccacaagc ttggcaggct tcaagtaata
     1201 aaggccaagg cgctcgtgtt cttgttttgg atacaggtat agatgctgaa catccttctt
     1261 tgaaagcaaa ctttgaaaaa ggtcgtgact tcactgggct cagtgatggg tctgacttca
     1321 gtgataaggt gggacatggt actcactgtt ctggaactat cgccggtgtt gaagaagctt
     1381 ctggcttttc tggcgtggct ccggaagcga aacttttgat gggacgtgtg tgttctgatc
     1441 aaggttgcag caacgttgcc atcgctcaag gtgtcaactg gggcattgaa gaaaaggtgg
     1501 atgtgatctc tatgtctctg ggggggatgt ggagtacgcc ggctgaaaaa acggcaatcg
     1561 cagcggcaga cgctgcgggt gtaacggttg ttgccgcttc tggtaacagt ggtactgcgg
     1621 ttgtgggcta tcctgcggct ttgccaacag ttatcgccgt gggcgcgatc aacgtaaaat
     1681 tggaaaaagc agatttctct caatacggtc cggagttggc agttgttgct cctggagtag
     1741 acgtggtttc ttctgttcct caaggtactg gtcgtgagtc tgaagtgatt atcactatga
     1801 acaattctcc gcaaagagtg gtgagcacaa cattccaagg cgctaaagag ctagatgtgc
     1861 agaatgatgt agaacttgtg gacgccggtt tggcgaagcc tgaggacttt gcagggaagg
     1921 atttcacagg gaaatatgct ttgatctctc gtggcgaaat cactttcggt gaaaaagtga
     1981 ataatgcgct gaaagcaaat gctgcgggcg ccattattta taacaatgct cccggtttga
     2041 ttcatggaag tttgactcaa gatggttcta ctttgaatat tgctgttgcc atgattgaac
     2101 aaactgtggg ccaagcattg aaggatgaat tgcttgcttc tcgtccagta ttagcttcta
     2161 tttccacggt gaaatcagac tatgcccatt tcgatgggac ttctatggca actccgcacg
     2221 tagctggggt ggcagctttg gttaaagcag ctaataagag tttgacacct gctcaagtta
     2281 aagaagtttt caagaaaaca gctcaggttt tgcctggtac taataccaaa aatgaattgg
     2341 gttcaggact tatcgatgcc caagcggcag taagtgcagc tctaggtcag tattagacct
     2401 taccaaaaga attagaaaga aaaggactct gtttagggtc cttttctttt tttgttcact
     2461 ccgctcagta tttaaacaat ccttttgata aacccttttc aagcctttat cctgtctgca
     2521 aaatagaggg atgacgacac atcttcctca aatcattcaa gatctcgcac ttattctggt
     2581 ggtagccgct accgtaagcc tgacttttaa aaaaatgaaa cagccagtgg ttttgggata
     2641 cctagtggcc ggtatgctcg tggggcctca tcaggattgg tttccaacgg tcagcgattt
     2701 tgaaggaatc aatgtctggg ctgatatcgg ggttatattc cttttattcg ctattggcct
     2761 ggagtttagc tttagaaaac tcttaagtgt cggtgcttct gccggaatta ccacggcatt
     2821 ccaggtcagt gtgatgctgc tgttgggttt tgttgccggt caatccatgg gctggagttg
     2881 gatgagtagc ttgttttttg gagcgacaat ctcaatttcc tcaacgacca ttgtggtgaa
     2941 gacctttgac gagctggatt tgaaaggacg ccgttttgtc agcctcgtca tcagtatttt
     3001 gattttcgaa gatttggttg ctgttttatt aatggttttt ttagcaactt tagcaatgag
     3061 tcgagagttt caaggaattc agttgttaga gcaaatcgca cgattggttt ttttcctgag
     3121 tctttggttt atggtaggtg tttttgtttt accaaatctt gtaaaacgta ttcgtcttct
     3181 tttgaatgct gaggccacct tgatcatttc cttaggactc tgttttttaa tggttgtgct
     3241 ggccagtaaa gctggttttt cgccagctct gggagctttt gtaatgggtt ctttgctggc
     3301 cgaaactagc gaaggggaac gcattgagcg ggttgtgact tcagtcaaag atcttttttc
     3361 agcaattttt ttcgtttcag tcggaatgct gattgatccc cgggttctca tggaacattg
     3421 ggcgagcgtt cttttgttga gcttgatgct gatttttggc aaagttttta gtgtcacctg
     3481 tggttctttg atttccggtc aaaatttaaa aacctctttg caggcgggat tgagcctggc
     3541 gcagattggg gagttttcat atattatcgc aggcctagga atgaccttga aagtgaccaa
     3601 tgaactttta tatcctctgg ttgtcaccat gtcaggcctc accacctttg ccactcccta
     3661 tatggtacga ggggctgatg gaatttactc ttggatcgaa aaaatagtgc ccattcatat
     3721 gcgtaaagca ttggatacct acagtctttt gccggccggt ggagggggcc ttgcgactcg
     3781 acgtgaatac ttaaaaaact tcggcttgaa gatgctttta aacacagtgg ttatcgtggc
     3841 tattttcttg gcgttctctg agggcctgtt gccctgggcg caacaaaaaa tagtaaataa
     3901 gggaatgacc cttatcattt gtctgaccct gagtttgatg gtttcggctc ctttcttttg
     3961 ggctttgatt tttagtgaag ttaagggcta cgccttttct ttaatttggc aaactcgtgc
     4021 cgttcgtacc tcccttttat ttctttcttt atttcgaagt gtttggggcg tgattttgtt
     4081 gtcagttctg ctgactcagt ttctcccgca atttcttccg atggtggtga tcctgctggc
     4141 gctttcgata ttcattttga ttttttacaa ccgcctggaa aggctgtacg gagccataga
     4201 ggggcgcttt ttaagcaacc tttatcgcaa gagccaagaa gaaaaagaag cccaagaaaa
     4261 ggagttggcg aatgccgaga tgtcgcccct ggctccttgg gatgcccact tggttgagtt
     4321 cgtgattcct caagaagcaa aatatgttgg tttccctctc gagagtcttt tgcttaggga
     4381 aaagtatgga gtcaccattg ctttgattcg tcgtggaggg caccgcatta ccgctcccgg
     4441 tcggcaggaa atgcttatgc ccctggatca catttttgtt atcggaactg atggacagct
     4501 attaaaattt aaacattttt tggattttga aagaatcagt gaagatattg caggctccgg
     4561 tcgcgattac accctggaac gttatttggt ctcggaaaaa tctccctttg tcggcaagtc
     4621 tatccgagcc tcaggccttc gcgaggccac gaatggcatt gtcgtaggca tggaaagagg
     4681 aggccaacgt attctaaatc ccgattccgg gataattatt gaggtggggg atttattgtg
     4741 gattgcaggg gatcgtaagt tgattaaagg tctcatttaa gatagaccct aagctattat
     4801 gactgaagag cgttttatcg aaattgagac taaaataacc caccaagaat ttctcattga
     4861 acaactcaac aaagtgattt acgagcaaca aggtaagata aaccaacttg aaacaatcct
     4921 gacggcgtta gtgaaacgaa ctcaggagat catgaatcca gaacctgaaa ttggaccagc
     4981 caacgataaa ccgcctcatt attagatgtg atttttattt aagtctgggg ctgttcttgc
     5041 cgatagagtc cctgatggct atgaaaaatt ccggcaaaat tcttattctg atatttcttt
     5101 ttgcgggcct tgtttcctgt cagaattcca ttccgacggt ggtcaacctg ggctctttgc
     5161 cgaagctttc atcccccgcc aattctaaga actttagtca gctcagtgcg gcctctcaaa
     5221 aggaagtctt agccagtggc tataagggaa cccttcgcat cagccaacct ttgggacgcc
     5281 tgcaagccac tacaagtgat ggacacaaac tgcaggggac cgtgacttta caacaataat
     5341 tcagcatcat ggaccatggt taaaatctct cacattctcg tcactcatca atatgaagcg
     5401 gaagatctgc tcaagctttt acaaaaagga aagagttttg aagatctggc tcgtcgtttt
     5461 tcaaaatgtt cctccgcaaa ggtgggtggc gatcttggtg agttaaagcc aggtcgggcc
     5521 gatgaggat
//
LOCUS       DATBTE010000106         1856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2199214, whole genome shotgun sequence.
ACCESSION   DATBTE010000106 DATBTE010000000
VERSION     DATBTE010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2199214"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1856
                     /locus_tag="VIG33_04055"
     CDS             <1..>1856
                     /locus_tag="VIG33_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEY8270035.1"
                     /translation="PGEIFISSIQALCQKTLPFKELKENSTIWSVGTELPEKIAGYLN
                     ALGYQSAPMVEDSGQYALRGGIIDIFSPAQERPIRIELFGDQIESLRFFSTLDQRSQE
                     EVASAAIIPAREVLFRDDHHQELLKKVRETLENRKIDRAEAEEVLRTLVLKNYFPGVE
                     FLLPSFYNKLETALDHFNSSLNIWLLDPIEISRFADELLAEMKKDFLAAETAVIRPNY
                     KEFFVAFDELELPTDSRQIYLSKLEYIENEAPDQIKIDYRSAPVTEFSNLSLSFSPGS
                     DPWLQAVKQKLVRWKDDHYKIFIGIKNQSQMERLGHIIERLDFKPIKCEAQDYLWDTW
                     IHNQEALNTTLHIIPRYLPESLRLEEEQIIFLREEDFFGRKQRVTESSSVEDFQKQAK
                     RLAFGDLKPGDLVVHVKHGIGIYEGLKTMPINGIDSEFIQIGYKDKDKLYLPVYRVGQ
                     LQKYSGATQTSILDKLGGIGWEKTKTKVKNQLRDIAHDLLVLYAKRAELYRPPITFKE
                     NEYELFENGFAYEETDDQLRAVRDIVKDLSANKPMDRLICGDVGFGKTEVAMRAAFFA
                     IQSQKQVAVLAPTTVLTFQHLETFKKRFQGWPVEIRALNRFITPADAKQTLK"
BASE COUNT          554 a          399 c          393 g          510 t
ORIGIN      
        1 aacccggaga gattttcatc agttctattc aggcgctgtg tcaaaaaact cttcccttca
       61 aagaattgaa agaaaactca acaatttgga gtgtaggaac agaacttcct gaaaaaatag
      121 ccggctattt aaatgctctc ggttatcaat cggctcctat ggtggaagac tctggccaat
      181 atgctttaag agggggaatc atcgatatat tttcccccgc acaagagaga cccattcgca
      241 tcgagctttt cggtgatcaa attgaaagcc tccgtttttt tagtaccctg gatcaacgga
      301 gccaagaaga agtggccagt gccgccatta ttcctgcgcg agaagttctt tttcgcgatg
      361 atcatcatca ggagctatta aaaaaagtcc gtgaaactct tgaaaatcgt aaaatcgaca
      421 gagccgaagc ggaagaggtc ttacgtactt tagtgctgaa aaactatttc cctggggtgg
      481 aattcttact tccctccttt tataataagc ttgaaacagc cttagatcat tttaattcgt
      541 cgctcaatat ttggttattg gatccgatag aaatttcacg ttttgctgat gagcttttgg
      601 ccgaaatgaa aaaggatttc ctagctgctg aaacagccgt gattcgccca aactataaag
      661 agttctttgt cgccttcgat gagctcgaac tcccgacgga ctctcgtcag atttatttgt
      721 ctaagcttga atacatcgag aacgaagctc cagatcaaat aaagatcgat taccgctcag
      781 cacctgttac agagttctca aatttaagct tatccttttc gccagggtca gatccttggc
      841 ttcaagctgt gaagcagaag ctcgtacgct ggaaagacga tcattataaa atttttatcg
      901 gcattaaaaa ccaatcacaa atggagcgtt tgggtcacat tattgaacgg cttgatttta
      961 aacctataaa gtgtgaagcc caagattacc tctgggatac ttggatccac aatcaagagg
     1021 ctttgaatac aaccctccat attattccac gctatttacc tgaaagtctg cgcctggaag
     1081 aagagcaaat tatcttccta cgggaagaag acttttttgg ccgcaagcaa agagtcactg
     1141 aatcctcctc tgtagaagat ttccaaaaac aagctaaacg cttggccttt ggagatttga
     1201 aacctgggga tttagttgtg catgtcaaac atggcattgg tatttatgag ggacttaaga
     1261 cgatgccgat taatggcatt gattccgagt tcattcaaat cggctataaa gacaaagaca
     1321 aattatatct ccctgtttat cgcgtcggcc agctacaaaa atattcaggt gccactcaaa
     1381 cctctatctt agataaacta gggggcattg gttgggaaaa aaccaaaacc aaagttaaaa
     1441 atcaacttcg cgatatcgcg catgacctgc ttgtgcttta tgccaaacgt gcggagcttt
     1501 accggccacc cataaccttc aaagaaaatg agtacgagct ttttgaaaat ggctttgctt
     1561 atgaggaaac agacgatcag ctacgggcag tacgcgacat agttaaagat ctctctgcaa
     1621 ataagccgat ggatcggctt atttgcggag acgtgggctt tggtaaaaca gaggtggcga
     1681 tgcgtgccgc tttctttgca attcaaagtc aaaaacaggt cgccgtgctt gcaccgacga
     1741 ctgtgttaac ttttcagcat ttagaaacat ttaaaaagcg ttttcaaggt tggcccgttg
     1801 aaattcgggc attgaatcgc ttcatcactc ctgcggatgc caagcaaaca cttaaa
//
LOCUS       DATBTE010000107         4919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2404530, whole genome shotgun sequence.
ACCESSION   DATBTE010000107 DATBTE010000000
VERSION     DATBTE010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2404530"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..3324)
                     /locus_tag="VIG33_04060"
     CDS             complement(250..3324)
                     /locus_tag="VIG33_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY8270036.1"
                     /translation="MNSEMGNIILRAGAGAGKTTTLTHLFLNYASDFKTTQRRFPRMV
                     VTTFTRKATQELKERLLHLALEQKREDLFHYVTNKSQVQISTIHGVLSLFLGRYGGNI
                     GLSSDFKLLSGPQETLMRRKILRRLILSQSDYKSEYESLLQSYSFHQLEGALQKYYEN
                     SFLFSDFQFIPKEEFAKDLQKQLSELEGLRHEVSSNILAEAQQPSWVKYAEGLQRISL
                     KKNADEVVQTLQIIKNFFADHPRKPSLSAKSPAVSSETGEMLAYLIEAMKDLLDSPES
                     LPEYWQEHEVKNRIFSELAQDFTSELLKSKIATGCITMADLELFSLKLIHESPEACES
                     FSSEWDYWMIDEYQDTSPTQVALLKAMVGDRKSFVVGDPQQSIYLFRGARSEVFQEKI
                     LATRESGGEVQEKLINYRSTPRLLHFINHYFLNSSDQFAAMEPSPEKKLGDIANPVAQ
                     LRLLEKAESKTDSTVETQAALERIQNLLAQGVDAEKICVLSRTRSGLEILAKEAEAYG
                     VPVQIHVAGGFYARREIRDALMFLKFLVNPHDNLNFLSCLRSPWLHLKDQEILNFCHS
                     DSYSFWSLAHKLIPEPDLRHPLALLKKYLQMSQGLGYSLVLRLMLIELGLLDYVQKLD
                     PTGRREANLWKLVADLFEQERTPGFNFLDFLSFQDIPDLEAGNEDSDATPVIAPRRVN
                     LMTVHASKGLQFDHVLLLGMTSKPRLSQAELFAVDEEKGQWSLSLMNEEERKIQPSRL
                     AIRVKEKFNRRELQEHQRVLYVALTRAKEGLTLLWIGKPEKNSWAAACPLQLSAGTFQ
                     EKEFSYSVVTGTFLPSQLVREEMEIQMDPGPWQSPVQTAAQQSPQQTVAVTQLIAASN
                     PSQQSQTQATVIPANILQKALAKAYRGTEAHRIFESLKYTSVEVVLTHLEDEEMKEAV
                     RFIVEAKEIPLAQIIERGFVEWGFSFQHQETWIQGQIDLWGKVDGGTWIVDYKTGSSD
                     NADTAFDQLKAYAWALYQMNFIASDEKVNLAVIYPFEKKIKFKPCVEITPNG"
     gene            complement(3333..>4919)
                     /locus_tag="VIG33_04065"
     CDS             complement(3333..>4919)
                     /locus_tag="VIG33_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY8270037.1"
                     /translation="AILVRDQFLAQALKAWQPHNAQQETQTDILVLVLREIFQNASGK
                     TEFSYKEWLTYLENILANKELKVTSASVEGVVISALMSAPSSRVPYKIFLGLCDENLK
                     NSQRGHILGADLNRLALDLGFYLEHPDHSYKDFQLRWLAESAGEKNIFSFGLADFSGA
                     LLSPSNFWMSLKPCDEVRLPGKTRWDALQGVPHKNILDLRGRSSTEQESFLARLEMDQ
                     GLTPVPPLELQQVPGLSASQIERYLQCPFVFTAQKIFKLQDHPELDLDEDARSKGTLV
                     HALFEKLTYPEFKADLSEEEIEQVLEDLCVQEKLTLADPRLWASMKKRYLQLAKRFLN
                     FEKEWRREFPETLTMAAEKAFEFYFNPQNGEVSREARAGTLKFSGKIDRIDKHRQTNQ
                     YVVIDYKSSHASYKHHNGWFENQQLQLLFYMWTLEKELLTELTGEVVGAFYYSFKNFN
                     REKGFQIEEHAGPLFRAAGRKGVKATNEDKRELLVKFESLILEVVTKIQKGEIEPRPF
                     DLKTCISCEWSVLCRAPHLN"
BASE COUNT         1331 a         1216 c         1040 g         1332 t
ORIGIN      
        1 tccgatctca gaaaagtcac caataagcag ttggaaacga ttgagtttga aatcaacaat
       61 agacctatga aaatccttga ctggaaaact ccttatgagg tcatgatgga aaaatagttg
      121 cactagagaa ttgcagtcag gattcattaa tttcattttt tcgaccgaca aacatatttt
      181 acaccaaata gactttatgc catttggtgt aaaatatagc acacgccaac aattacacca
      241 aatagtattt taaccatttg gtgtaatttc aacacaaggc ttaaatttga tcttcttttc
      301 gaaagggtaa atcacggcca ggttcacttt ttcatccgaa gctatgaaat tcatttgata
      361 caaagcccag gcataagctt tcagttggtc aaaagccgta tctgcattgt cactggatcc
      421 cgttttataa tccacgatcc aagtgcctcc atcaactttt ccccaaagat ctatctggcc
      481 ctgaatccaa gtttcttgat gttgaaaact gaacccccat tccacaaagc cccgctcaat
      541 aatttgggct aaaggaatct ccttggcctc aacgataaat ctgacggcct ctttcatttc
      601 ttcgtcttcc agatgagtaa gaacaacttc tacggaagtg tacttgagag attcaaaaat
      661 gcgatgagct tcggttccgc gataagcctt ggccaaggct ttttgcaaaa tgttcgccgg
      721 gatcactgtc gcctgagttt gggattgttg actagggtta gacgctgcaa tcaactgagt
      781 caccgccacc gtctgctgag gagactgctg agctgccgtc tgcaccggac tttgccaagg
      841 ccctggatcc atttgaatct ccatttcctc gcgcacaagt tgagagggta aaaaagttcc
      901 cgtaacaaca ctgtaactga attctttttc ctgaaaggtt ccggcactca attgcaaagg
      961 acaagcggcg gcccaggagt tcttttctgg tttcccgatc cacagcaaag tgagaccttc
     1021 tttcgcgcgg gtcagcgcca cgtaaaggac ccgctgatgc tcttgcaatt cgcgccgatt
     1081 gaatttctct ttcacccgaa tcgccagacg cgaaggctga attttgcgtt cttcttcgtt
     1141 cattaaagat aaagaccatt gacctttttc ttcgtccacc gcgaaaagct ccgcctgact
     1201 gagtctcggc ttggaggtca tccccaaaag caagacgtga tcaaactgca agccttttga
     1261 ggcatggacg gtcatcagat tcacccgtcg cggcgcaatc accggcgtcg catctgaatc
     1321 ttcattcccc gcctctaaat ccggaatgtc ttggaaagag aggaaatcta aaaagttaaa
     1381 tcctggcgtg cgctcttgct caaacaaatc cgctaccaat ttccacagat tggcttctcg
     1441 ccgtccggtg ggatcgagtt tctgcacata atccaaaagc ccgagttcaa tcaacataag
     1501 gcgtagaacc aaactataac ccagcccttg ggacatttgg agatactttt ttaagagcgc
     1561 gagcggatgg cgaagatccg gttctggaat caacttgtgg gccaggctcc aaaatgagta
     1621 agaatcacta tgacagaagt tcaaaatttc ttgatccttc agatgcaacc agggtgaacg
     1681 cagacagctc agaaaattaa gattgtcatg gggattcacc aaaaacttta aaaacatcag
     1741 cgcatcccgg atttcacgcc gagcatagaa gccccccgcc acatggattt gtacgggcac
     1801 gccataggct tccgcttctt ttgctaaaat ctccaaaccg ctgcgagtgc gggaaagcac
     1861 gcaaatcttt tccgcatcca caccttgagc tagtaaattt tgaattcttt ccagcgccgc
     1921 ttgagtttcc acagtggaat cagttttgct ttctgccttt tccagcaagc gaagttgggc
     1981 cacgggattc gcgatgtcgc caagcttctt ttccggagaa ggctccatgg ccgcaaactg
     2041 atcactgctg tttaaaaaat aatgattaat aaaatgcaaa agccttggag tggaacggta
     2101 atttatcagc ttttcttgaa cttctccgcc gctttcgcgg gtggccagaa ttttttcctg
     2161 aaaaacttct gaacgcgccc cgcgaaacaa ataaatactt tgctgcggat ctcccacgac
     2221 aaagctttta cgatccccca ccatggcctt caacagcgcc acttgagtgg ggctcgtgtc
     2281 ctgatactca tcgatcatcc agtaatccca ctcagaggaa aaactctcac aagcttccgg
     2341 agattcatgg atcagcttca ggctgaataa ctccaggtcc gccatggtga tacaacccgt
     2401 ggctattttc gacttcaaca actcactggt aaaatcttga gccagctcag agaaaatccg
     2461 atttttgacc tcatgttctt gccaatattc gggcaaggac tctggagaat ccaaaagatc
     2521 cttcatcgcc tctatcaaat acgccaacat ttcgcccgtt tctgaactga cagcgggaga
     2581 tttggcgctc aaagaaggtt tgcgtggatg atccgcaaaa aagtttttga tgatttgaag
     2641 ggtttgaaca acttcgtccg cgttcttctt tagagaaatg cgttggagtc cctctgcata
     2701 cttcacccaa gaaggttgtt gagcttcggc taagatgttg ctgctgactt cgtgacgtag
     2761 accctccagc tcgctcagtt gcttttgtaa atctttggca aattcctctt ttggaataaa
     2821 ttgaaaatct gaaaaaagaa aagagttttc ataatatttt tgcaaggctc cttcaagctg
     2881 gtgaaagcta taactctgca gcagagattc atattcagat ttatagtcac tctgcgaaag
     2941 aatcagccga cgcaaaattt ttctgcgcat cagagtctct tgcggccctg aaagcagttt
     3001 gaagtctgaa ctcaagccaa tattaccgcc ataacgaccc aaaaaaaggc ttaaaacacc
     3061 atggatggtg gaaatttgca cttgcgattt attggtcaca taatgaaaaa ggtcttcgcg
     3121 tttttgctcc aaggccaaat gcaaaagccg ttctttcaac tcttgcgtgg ctttacgggt
     3181 aaaagtggtc actaccattc ggggaaatcg ccgttgagtg gttttgaagt ctgaagcata
     3241 gttcaaaaat aaatgcgtca gggtcgtggt cttccctgcc ccggctccgg cgcggagaat
     3301 aatatttccc atttcagagt tcatttcagg cttcaattca gatgaggagc tcggcacagc
     3361 acactccatt cgcaggaaat acaagttttc aaatcaaaag gacgcggctc aatctcgcct
     3421 ttttgaatct tcgtcaccac ttctaaaatc aggctctcaa acttcactaa aagctcccgc
     3481 ttgtcttcat tcgtcgcctt gacccctttt cgtcccgccg cgcggaacaa ggggcccgca
     3541 tgctcctcta tttgaaaacc tttttcgcgg ttgaagtttt tgaagctgta ataaaaagct
     3601 ccgaccactt ctccggtcaa ttccgtcagc aactcttttt caagggtcca catataaaaa
     3661 agcaactgca gttgctgatt ttcgaaccaa ccgttgtggt gcttatagga ggcatgactg
     3721 gacttgtaat caatcaccac atattgattt gtttgacgat gcttatctat acggtcaatc
     3781 tttccactaa atttcaaagt ccctgcccgc gcttcgcggg aaacttcgcc gttttgcggg
     3841 ttaaaataaa actcaaaggc cttctcggcg gccatggtta aagtctccgg aaattcgcgg
     3901 cgccactctt tttcaaaatt aagaaaacgt tttgccaatt gcaggtatct cttcttcatc
     3961 gaagcccaaa ggcgcgggtc cgccaacgtg agtttttctt gaacgcaaag gtcttcgaga
     4021 acttgctcta tttcttcctc tgacagatcc gctttaaact ccggataggt gagcttttcg
     4081 aataaggcgt gaaccaaggt tcctttactg cgcgcgtctt cgtcgagatc taattctgga
     4141 tgatcctgga gtttaaaaat tttctgtgcg gtaaaaacaa aagggcactg taaatatctc
     4201 tctatttgcg acgctgaaag ccccggcact tgctgcaact ccaggggcgg cactggcgtc
     4261 agtccttgat ccatttcaag gcgtgccaaa aacgattctt gctccgttga agagcggccc
     4321 cgcaagtcta agatattttt atgcggcact ccttgcaggg catcccaacg tgttttcccg
     4381 ggaaggcgga cttcatcgca gggttttaga ctcatccaaa aattcgatgg cgataacaaa
     4441 gccccagaaa agtcagcaag accaaagcta aaaatatttt tttctccagc gctttcggcc
     4501 agccagcgca attggaaatc tttataggaa tgatcaggat gttccagata aaaacctaaa
     4561 tccaaagcga ggcgattcag atccgcacct agaatatgac cgcgctggct atttttcaga
     4621 ttttcatcgc aaagtcccaa aaatatttta taaggcactc gagaagatgg tgcggacatt
     4681 aaagcggaaa tcaccacgcc ctcaacggaa gcactcgtga ctttcaattc tttattggcc
     4741 agaatatttt ccagataagt cagccattct ttgtaactaa actcggtttt accggacgca
     4801 ttttgaaaaa tctcccgcaa cacgaggacc agtatgtcgg tttgagtttc ctgttgcgca
     4861 ttgtggggct gccaagcttt taaggcctgg gctaagaact gatcccgaac aaggattgc
//
LOCUS       DATBTE010000108         2349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1858027, whole genome shotgun sequence.
ACCESSION   DATBTE010000108 DATBTE010000000
VERSION     DATBTE010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858027"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(583..1941)
                     /locus_tag="VIG33_04070"
     CDS             complement(583..1941)
                     /locus_tag="VIG33_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270038.1"
                     /translation="MIFTAPRFVVVDDKIEHLEAIVQAFQQLGSPCIGIQYKPEEELD
                     ANIFRGVRVLFLDLHLIDSSVKTDDRPHYAVIAGILEDKIHVNGGPFVLVLWTEHAHC
                     AQGLTEYLDMGLDKKKPHSRPLVVVPLSKDNLINKDDKVTDPEELRRKVREAVTANAQ
                     LAALLDWETDVMTAAGDTLVALLNLVPTENRTNTSYSDALDVILSRLACEAVGKPHVE
                     VNQRAAITTALAPILIDRMMNQEISKETLDLWSKAITRHSGKKLEDASPEEAGHLNKM
                     LHLAIPGSETVRPADWGAVVRWPYEWNDDELLCRMGFKIPDMLGGQFLIERKDRARCK
                     PHLVRIGAACDYAQNKEGPITYLLAMEIPEDAERKKNRKTGDDLELSDAIWRSPVFIT
                     TDSAESFRLYVHIRFPISVLPSMTDGWFASYRMREQLLMNLLTFSSNYSSRPGIVQLP
                     IK"
     gene            complement(1938..>2349)
                     /locus_tag="VIG33_04075"
     CDS             complement(1938..>2349)
                     /locus_tag="VIG33_04075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8270039.1"
                     /translation="LEDEVPDIESDFSFGLLLGALNNLLDNAFYWLQVRWPEDDNNSE
                     REIYINFEKDFAEGAAIIVADNGPGLQDEPDHLTRPFFSRRPDGMGVGLYYTNMVMEL
                     NGGRLAFPDADEASVPERFDGAVLALVFGKERRK"
BASE COUNT          646 a          572 c          494 g          637 t
ORIGIN      
        1 ctagattcac aagcatatca ggagctggtg caagatgggc atccagtcgt agtcatttcg
       61 gcaggagatt tggcgcttaa gttgaaggaa aagtttggaa gtctggataa agtcaaaact
      121 tggttagaga gagtttgatt ggattgggtc agatttacag gtatccattc ccaaaaatga
      181 agaaaggcga taagtcaacg tagacaggtc aactgtttca cactcccata ttaccagaac
      241 gatccaacca agtttttgca gttttttcat agtctcgcgg tcacgctcga cattacgggt
      301 aaatttcttt ttccagaatg ccaaattcga cttgggggaa tacgagtatc tgcatccctc
      361 atgacgatgc caaaaacaac catgaacaaa gatcaccttt ccaagccgag gaaacacaag
      421 gtctggtcta ccaggtaaat ctcgtcgatg aaggcggaaa cggtatccaa gcgcatgagc
      481 cgcacgtctg acaatcattt ctggcttcgt atccttactt cggatacgtg acatcattgc
      541 ggatcgctga tcaggcgtaa gaatgtccac catgctttac agtcacttga tcggcaactg
      601 tacgattccc gggcgagaag aatagttgct tgaaaatgta agcagattca tcaaaagctg
      661 ttcgcgcatt ctgtaacttg caaaccaacc atcagtcata ctcggaagta cactaatcgg
      721 aaaccgaata tgtacatata ggcggaatga ctctgcacta tcagtggtta taaaaactgg
      781 cgagcgccat attgcgtctg acagttccag atcatcacct gtttttctat tctttttcct
      841 ttctgcgtcc tcaggtattt ccatggcaag taaataagtg attgggcctt ctttattttg
      901 tgcataatca caagcagcgc ctattcttac cagatgaggt ttgcatctcg ccctgtcttt
      961 ccgttcgatc aggaattgcc cgccaagcat atcagggatc ttaaatccca ttctgcacaa
     1021 aagttcatca tcgttccatt catacggcca tctcacgaca gccccccaat cggcaggtct
     1081 gaccgtttct gagccaggaa ttgccaagtg cagcatttta tttaaatgac cagcctcctc
     1141 tggcgaggca tcttctaatt tcttgccact atggcgggta atggctttgg accagagatc
     1201 tagtgtttcc ttggatattt cttggttcat cattctgtca atcaaaattg gagccagcgc
     1261 agtggtaatt gctgcacgct ggttaacttc aacatgtggc tttccaacag cctcgcatgc
     1321 caaacgactg agaatcacgt caagcgcatc tgaatacgag gtattcgtcc tattttcagt
     1381 gggaaccaga ttcaagagag cgactagtgt atcgccagca gctgtcatta catcagtttc
     1441 ccaatcaagc agagcagcaa gttgagcatt tgcagtgacc gcttctctca ctttccttcg
     1501 aagttcttcc ggatcagtga ctttatcgtc tttgttgatg agattatctt ttgaaagcgg
     1561 aaccacaaca agtggacggg aatgcggttt tttcttatcc agccccatat ccagatattc
     1621 cgttaatccc tgggcacagt gagcatgttc tgtccaaagc accaaaacaa acgggccgcc
     1681 gttaacatga attttatctt ccagtatccc tgcaatcaca gcgtaatggg gacgatcatc
     1741 agtttttaca cttgagtcaa tcaagtgaag atcaagaaat agcacacgaa caccacgaaa
     1801 gatgttcgca tccagttcct cttccggctt atattgaata ccaatgcagg gagatcctag
     1861 ttgctgaaat gcctgaacga tggcctctaa atgttcaatc ttgtcatcga ctacgacaaa
     1921 acgcggtgct gtgaaaatca ttttcttctc tcttttccaa aaaccagtgc aagcacggca
     1981 ccgtcaaagc gttcaggaac actagcttcg tcggcatctg gaaatgccag tcttccccca
     2041 ttcagctcca tgaccatgtt tgtataataa agtccgaccc ccattccgtc aggacgtcga
     2101 ctgaagaacg gacgagtcag atgatcaggc tcatcctgta gaccaggacc attgtcggca
     2161 acaatgatcg cagcgccttc agcaaaatct ttctcgaaat taatgtaaat ttcgcgttcg
     2221 gagttgttgt catcttcagg ccaacgcact tgtagccagt aaaatgcatt gtccaaaaga
     2281 ttatttaatg caccaagcaa caagccgaat gaaaaatcgc tctctatgtc aggcacctca
     2341 tcctccagt
//
LOCUS       DATBTE010000109         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2404767, whole genome shotgun sequence.
ACCESSION   DATBTE010000109 DATBTE010000000
VERSION     DATBTE010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2404767"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(744..1178)
                     /locus_tag="VIG33_04080"
     CDS             complement(744..1178)
                     /locus_tag="VIG33_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270040.1"
                     /translation="MRTIPTFKKIQLSTKPSTGSMENILTPVNLSGIIVPWNKALGSG
                     RESDFKLVCSNGHEYFMAADSEWRQTLPTYCWEEVKVIGLLNLSNMTLIPQKIFPKGP
                     RGEMENLVALARGRGQVLARKLVKNINDLVIVPAAIWAVMAF"
     gene            complement(1195..>1538)
                     /locus_tag="VIG33_04085"
     CDS             complement(1195..>1538)
                     /locus_tag="VIG33_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEY8270041.1"
                     /translation="DEILMYEFHIRELTQTEALKQYSDHAVKEIKSIYGWDSEVQVKI
                     EPEAKDKHLFSVSISVFGLGQAIVVKKSGKHVMAALRKVRKAVMRQIHRLNERRISCR
                     KKTFFKEQFAS"
BASE COUNT          446 a          346 c          286 g          460 t
ORIGIN      
        1 taaaaagatc tatattttga tcttcacaga agatctttac caaagaagac tttccaacct
       61 gccgagcccc ccttaaaacc aggggttttc ggattgctga attataccat tctttaataa
      121 attcaatatc ttgacgtttc attgcttaat tgtatcaatt ttgtgataat ttatataggt
      181 gttttatcat acaaaatgct atttttactg gatatatcag tatcatattg attttattag
      241 ttataattgt ccatctttgc tcatgcagag ataaatcact agcctgttca gctctctctg
      301 atttttagga aggagagaga gtatgttaaa acgccccagg atagttcctc gcaaaaaact
      361 cttctacttc cttcgcatct gcccctaaga accctctcaa tttcgggatc aacaacaaaa
      421 ggggatttaa taaaataagg ccgcctccca taaggaccaa ggatttggtc aaccccatgg
      481 cagaaatcaa gattcccgcg ccagcaactc cgagagggga aattccagtg caaagaaaat
      541 tcatgattga gccgattctc gataaatagg aatttggaac agagagtgaa atttgtgcgc
      601 ctaacggaat attcgcccat gaactcccaa taccaatcca gaataaaacc gctaactggt
      661 ttcatagttc aaagcattga tatcaaaatg ttgaaacgaa gggccaatcc aatcaggcct
      721 tggccccaag ggatcagata acgctaaaag gccatcactg cccaaatcgc ggcaggcacg
      781 atcaccagat cgtttatatt tttaactaat tttcttgcca gtacctgccc tctaccccta
      841 gcaagagcta ctaagttttc catttcgcct cttggtcctt ttggaaaaat cttttgtggg
      901 ataagagtca tgtttgagag attcagtaaa ccgatcactt taacttcttc ccaacaatag
      961 gtggggaggg tctgtcgcca ctcggaatca gcagccataa agtattcatg gccattggaa
     1021 cagacgagct taaagtccga ttctcgcccg gagccaagcg ctttgttcca gggaactatg
     1081 atcccggaca aattcacagg agtcaaaata ttctccatgg agcccgtgga aggcttcgtt
     1141 gaaagctgta tttttttaaa tgtagggatt gttctcataa agtcctcctc tcggctaaga
     1201 agcaaattgc tccttgaaaa aggttttttt tctgcaactg attcgtctct cattgagtcg
     1261 atgtatttgt ctcatgacag cctttcgcac ttttcgcaag gcggccataa cgtgctttcc
     1321 gcttttttta acaacgatcg cttgacccaa accaaataca cttattgaaa cagaaaataa
     1381 atgtttgtcc ttggcttcag gttcaatttt tacttgcacc tcactgtccc agccgtaaat
     1441 ggatttaatt tcctttacag catgatctga atactgtttg agcgcttctg tttgcgtaag
     1501 ctccctgatg tgaaactcat acattaaaat ctcatcgg
//
LOCUS       DATBTE010000110         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_833053, whole genome shotgun sequence.
ACCESSION   DATBTE010000110 DATBTE010000000
VERSION     DATBTE010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833053"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VIG33_04090"
     CDS             complement(<1..378)
                     /locus_tag="VIG33_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270042.1"
                     /translation="MLSIYPNLTFAQKKPSKSQVPSEGKGGFKIHIDPEEVNRIIEEL
                     INSPNDAFEHDAKNAIEPGTIDLHPDGTVSAYPRPPQVKPIKHDPVQIDLQSDGSYEV
                     VPPTPTTGFYLVSFPKLTSIDHEA"
     gene            complement(656..1138)
                     /locus_tag="VIG33_04095"
     CDS             complement(656..1138)
                     /locus_tag="VIG33_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270043.1"
                     /translation="MQSIRPSRTNLAFGARHQGPLRSPQPRQQEFIELRSDGTYEVIS
                     AAFKSVSPILKLADRLVNLSSDESSSLFKAFRKFDRRSRYAKAVTPPRASDNTEDDAK
                     CQPSEGDEFQLRKRPPVTIMLSPEDYRVISREYEPIPQCSDELVVKLELPFLPNKKQD
                     "
     gene            complement(1362..>1865)
                     /locus_tag="VIG33_04100"
     CDS             complement(1362..>1865)
                     /locus_tag="VIG33_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270044.1"
                     /translation="FVKVQCNQRDSNMLSIYPNLTFAQKKRPKPEIPSERFSDFAGNP
                     DTVQKVRKLLGGNPARPQTDTLEFDTSALGRKGKPTQRERIPETIELNSDGSCKFDPH
                     TYRALALDNGLFALISKSDLDRWTRVEKDITPQHAASQIVFTNLGDWLQSEEIEDGVF
                     FEQHEQK"
BASE COUNT          478 a          445 c          416 g          526 t
ORIGIN      
        1 cgcttcatga tcaatgctag tcaacttggg aaaagacact aaataaaatc ctgtcgtagg
       61 agtcggggga acaacctcgt atgagccatc cgactgcaaa tcaatttgta ccggatcatg
      121 ttttatgggc ttgacttgtg ggggccgtgg atatgcagaa actgtaccat ccgggtgcag
      181 atcaatggtt cctggctcta tagcattctt ggcgtcatgc tcaaaagcat cgttggggct
      241 gtttatcaac tcttcaataa ttctatttac ttcctctgga tcaatatgaa ttttaaaacc
      301 gccttttccc tcactgggga cttgcgattt cgacggcttt ttttgggcaa aggtcaagtt
      361 aggataaatt gacaacatgt tggaatccct ctgattgcat tgcaccttaa caaaccttgt
      421 gattgcaaaa atttgccccc aaatttattc ctaaacagga atgaacagaa ttctctgtca
      481 ctacttggct aagtaaaaaa acggaagagg caatcaggcg tgggtcgtag aggtttcgcc
      541 ttgcgcaata tgggcgacat cttctccggg gacttccaac ggaatccaga agccgaaggt
      601 gctgccgacg ccgacttcgc ttttcagccc cggtagcccc tcaaaaattg gaaagttaat
      661 cctgtttttt gttgggcaga aacggcaatt ccaactttac caccaactca tccgaacatt
      721 gaggaatcgg ctcatattca cgagaaatca cccgataatc ctcgggagac aacataatag
      781 tgacgggtgg ccgcttcctc agttgaaatt cgtccccctc ggatggttgg cactttgcgt
      841 cgtcctctgt gttgtcggag gccctgggcg gcgttacagc ttttgcgtat cgactacgcc
      901 ggtcaaattt tctgaacgct ttaaatagcg acgaagattc atccgatgaa aggttaacca
      961 atcgatcagc cagtttcaat attggtgata cggacttaaa agctgctgaa ataacctcat
     1021 atgtcccatc cgagcgcaat tcaatgaatt cctgttgcct tggctgcggg gaacgtagcg
     1081 gcccttgatg tctggcgccg aaggcgaggt ttgtgcggct ggggcgaatg gattgcatgc
     1141 cagttaaccc tcctcgctgt aaatcatagc gcaataaaaa cgttgaaacc tcaaattttc
     1201 ctttgtctat tacgtaaaat tcaaggcccg ttcagggtct ttttcttttg gcgcaacggc
     1261 ttcagcaaaa tcaagccaat aactgagaga ctgacaacta agcggatcct agttcagtat
     1321 ctgtaaccag cctgcaaagt ctttcagcac gattcagtta tttatttctg ttcgtgttgc
     1381 tcaaaaaaca ccccatcttc tatttcctct gactgcaacc agtcacccaa attcgtaaaa
     1441 acaatttgtg atgctgcatg ctggggtgtg atatcttttt cgacgcgggt ccagcgatca
     1501 agatcgcttt tcgaaattaa tgcaaataac ccattgtcca aagctaaagc tctgtaggta
     1561 tgtggatcga atttgcacga tccgtctgaa ttcagctcta tcgtttccgg gattctctcc
     1621 ctttgagtag gcttaccctt tctccccaat gccgaggtat caaattccaa agtatctgtc
     1681 tgtgggcgcg ccgggttacc gcccaacagt ttacgaacct tctgaacagt atccggattg
     1741 cccgcaaaat cactgaatcg ttcgctggga atttctggtt tcgggcgctt tttttgggca
     1801 aaggtcaagt taggataaat tgacaacatg ttggaatccc tctgattgca ttgcacctta
     1861 acaaa
//
LOCUS       DATBTE010000111         5069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1243302, whole genome shotgun sequence.
ACCESSION   DATBTE010000111 DATBTE010000000
VERSION     DATBTE010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243302"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VIG33_04105"
     CDS             <1..323
                     /locus_tag="VIG33_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270045.1"
                     /translation="FHEVLKPTPINQKSVFIHQFVGMTDIKFDYDDFEKVRISLISKM
                     SQSLSNSDKDFLISFSLGDPQWEIAAISKMKELSAVKWKLLNIKKMSEKKRKDMTQML
                     RASF"
     gene            complement(331..768)
                     /gene="ybeY"
                     /locus_tag="VIG33_04110"
     CDS             complement(331..768)
                     /gene="ybeY"
                     /locus_tag="VIG33_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY8270046.1"
                     /translation="MKILVINQSSVRTPRKFIEAWILQLQKEFLKRKIFDKNSASKEL
                     TLVFLNPKEAKATNKQFRSKAYATDVLSFDGMSPDSLGELILCPQVLQKQAKVHGLKY
                     QEELGYMILHGILHLLGYDHETGVANAEEMFGIQDEVFAKLLK"
     gene            complement(765..3101)
                     /locus_tag="VIG33_04115"
     CDS             complement(765..3101)
                     /locus_tag="VIG33_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HEY8270047.1"
                     /translation="MKFLEWASHLGFEKTFLSRFFYNLYEELERRFYLRRVTTIFLFC
                     WALSYLIFFQLELPYDFRVGDVAKVEMVSPFSFEMTDEVTTEEKRLRAEQTVPVVYDY
                     DTSVFERVSSGIYRSFRAQRAYLREFKWPKTNKGHEEAMRDFFQHKPQFEKELGVGIS
                     DFMYEWLVENKFSPRIESAVVRNLENWYEKKIAEAPDRFIPSNQTTVLARVIHKSNLG
                     REFHLAKMDILDLQNPEHFDAEISPSLDKMTESDRANILYLSRSLVVPNLTLNKQEAT
                     SRKQSAREAVLPVNITIKKNQVIVPQGSVVQPFQMAMIRQIQAIQADRRGNLMTFSLA
                     LFLTLLIIVFVSYMRRFTLNKVRIPPKDSLVMMLIVLTVVLFTKLYLFITEAAFASKF
                     GHILTPKVFLYAAPVAAAPMMMGLLVTSGEVVWLFTAFMAICVGIMTDFSFNFMLVTM
                     VGGIAGARGVFMCKTRNDIYSTGVRTGLVNALLVVFLLTVSKIEQEGTLNEVYVTVPA
                     AFIGGIFSALLTMMIIPMLESLFNYTTDVKLLELSNLNHPLLKEMIVKAPGTYHHSMM
                     VGSMVEAAAEDIDANPLLGKVICYYHDIGKTAHANYFIENQKPGQNPHDHISSFMSKT
                     LLIAHVKDGVELGMKYKLGKPIIDGILQHHGTTLISFFYNKALDLKKSDDCEISEEDF
                     RYPGPKPQFREAALCMLADSIEAAARSLDEPTPTRLQNIVRNVIQRKLQDNQLDQCNL
                     TLKDISKVEEAFIRILLGIYHQRIDYPRSAGGGLGEKA"
     gene            3286..4203
                     /gene="mazG"
                     /locus_tag="VIG33_04120"
     CDS             3286..4203
                     /gene="mazG"
                     /locus_tag="VIG33_04120"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEY8270048.1"
                     /translation="MPKGPENLKDIRECMQVLMNIVTDLRGPNGCPWDKEQTHNTLAR
                     YAIEETFEMVEALEEREDCRRKLGQAPSKEEENKNRNSFVSLSTKFKEELGDVLFQVV
                     LHSQLASEEGSFDFSDVVISICEKLVRRHPHVFGDVIVKNSEDVIKNWNNIKKEEKAK
                     RGEKASLISVPPGLPALQRAFTIGEKTHRINFDWQGPQEVWLKVEEEIAELQEAIDND
                     VMSEIEHELGDVLFSLAQLARHYELDPEQVLRAANTRFLGRFEKMIGEYRKAKANTSD
                     VDEVLKKFGALSTEEKENFWELAKRNFGG"
     gene            4205..4999
                     /locus_tag="VIG33_04125"
     CDS             4205..4999
                     /locus_tag="VIG33_04125"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY8270049.1"
                     /translation="MRQYHELLKHVLVHGVQKEDRTGTGTLSVFGYQMRFNLEESFPL
                     LTTKKLFTRAIFHELLWFLKGDTNIKYLRDNKVTIWDEWADAEGNLGPVYGKQWRSWE
                     APDGRKIDQISNVVEQIKKNPDSRRLLVIAFNPADVDKMALPPCHAFFQFYVANGKLS
                     CQLYQRSADIFLGVPFNIASYALLVHMMAQVCNLGVGEFIHTLGDAHIYSNHLEQVKA
                     QLQREFRPLPKLSLNPAVKNIFDFKFEDIQLTGYDPHPAIKGEVAV"
BASE COUNT         1478 a         1192 c         1076 g         1323 t
ORIGIN      
        1 ccttccacga agttcttaag ccaacaccta tcaaccaaaa gtcagtattc atacatcagt
       61 ttgtgggaat gactgatatc aaattcgatt atgatgattt cgaaaaagta agaatctcgc
      121 ttatttcaaa gatgagccag agtcttagta attctgacaa ggatttttta atctcctttt
      181 ctttaggcga tccgcaatgg gagattgcag ctatttcgaa aatgaaagag ctgtcggcgg
      241 tcaaatggaa acttttgaac attaaaaaaa tgtcagaaaa aaaacgaaaa gatatgacac
      301 aaatgttgag ggcttcattc tgattcctcc tcacttcagc agcttcgcaa agacctcgtc
      361 ctggatgccg aacatttctt cagcattcgc gacccccgtt tcgtgatcgt agcctaacaa
      421 atgcaaaatg ccatgaagaa tcatataacc cagctcttct tggtacttta aaccatgcac
      481 tttcgcctgt ttttgtagaa cctgagggca aagaattaat tcacccaaag aatccggaga
      541 cattccatca aagctcaaga catcggtcgc gtacgctttg ctccggaact gtttattggt
      601 cgccttggct tctttcggat tcaaaaaaac caaggtcagc tctttggatg ccgagttttt
      661 gtcgaagatt ttgcgcttca gaaattcttt ttggagctgc aaaatccaag cttcaataaa
      721 tttgcgcgga gttcgcacag agctctgatt gatcaccaga attttcatgc tttttcgccc
      781 aacccacccc cggcgctccg tggatagtca atccgctgat gataaattcc caaaagaatt
      841 cttataaagg cttcctctac ctttgaaatg tctttcaagg tgagattgca ttggtccaac
      901 tggttatcct gaagctttct ttgaatcaca ttgcgtacaa tattctgcaa acgcgtgggt
      961 gtgggttcgt ctaaagagcg agccgcggct tcaatactgt ccgccaacat gcataaagcc
     1021 gcttcacgga actgaggttt tggtcctgga tatctgaaat cttcttctga aatttcacaa
     1081 tcatcggatt ttttaagatc caaggccttg ttatagaaaa aggaaatcaa ggtggtgccg
     1141 tgatgctgga gaatcccatc tataatcggt tttcccagct tatatttcat ccccaattcc
     1201 actccgtcct taacgtgagc gatcaataaa gtcttgctca tgaaggacga aatatgatca
     1261 tggggattct gtcccggctt ttgattttca ataaaataat tggcatgggc ggtcttcccg
     1321 atgtcatggt agtagcaaat gactttgccc aagagcgggt tggcgtcgat gtcttccgct
     1381 gcagcctcga ccatgcttcc gaccatcatg gaatgatggt aagtgcctgg agctttaacg
     1441 atcatctctt tgagcagagg atgattcaaa ttgctcaatt caagaagttt cacatccgtg
     1501 gtgtaattaa acaaggactc cagcattggg ataatcatca tagttaaaag cgcgctgaaa
     1561 attcccccga taaaggccgc tggaaccgtc acatagactt cattcagcgt gccctcttgc
     1621 tcgattttac tcacggtcaa aaggaagacc accaaaagcg cattaaccag gcccgttcgc
     1681 actcctgtcg aataaatatc gttgcgggtc ttgcacatga agactccgcg ggctccggca
     1741 attccaccca ccattgtcac tagcataaaa ttaaaactaa aatccgtcat gataccaaca
     1801 cagatggcca taaaggccgt gaaaagccac accacctcac ccgaagtaac gagcagaccc
     1861 atcatcatcg gagccgccgc cacaggggcc gcatacaaga atactttggg agttaaaatg
     1921 tgcccgaact tcgaagcaaa ggcggcctcc gtgataaaaa ggtaaagctt cgtgaacaac
     1981 acaactgtca atacaatcaa catcatcacc agcgagtctt tcggcggtat gcggacttta
     2041 ttcagtgtaa aacgacgcat ataagaaacg aaaacaataa ttagcaaagt caaaaacagc
     2101 gccaaagaaa aggtcatcaa attgccccga cgatccgctt gaatcgcttg gatttgtcga
     2161 atcatggcca tttgaaaagg ttgaacaacg cttccttgag gaacaatcac ttgattcttt
     2221 ttaatggtga tattcactgg cagcacggct tcgcgagcgc tctgtttgcg cgaggtcgct
     2281 tcctgcttat tcaaagttaa attcggcacc accagcgagc gagaaagata aagaatattc
     2341 gcccggtcac tctcagtcat tttatccaaa gaaggcgaaa tctcggcatc aaagtgctct
     2401 ggattttgca gatcaagaat gtccatttta gcaaggtgaa actcccgtcc aagattgctc
     2461 ttgtgaatga ctcgagccaa aacggttgtt tgattggagg gaatgaaacg atctggagct
     2521 tcagcgattt ttttctcata ccaattttcg agattacgaa ccacggcgct ctcgatgcga
     2581 gggctaaact tgttctctac cagccactca tacataaaat cagaaatgcc aacgccgagt
     2641 tctttttcaa actgtggttt atgctggaaa aaatcccgca tggcttcttc gtgtccctta
     2701 ttggttttag gccacttaaa ctcgcgcaga taagctcgtt gagcacggaa agaacgataa
     2761 atcccgctgg aaacccgctc gaaaacagag gtgtcgtaat cataaaccac cggcacagtt
     2821 tgttcggccc gaagacgttt ttcttcagtg gtcacttcat ccgtcatttc aaaactaaag
     2881 ggagaaacca tctccacctt tgccacatcc ccgacacgaa agtcataggg cagctcaagc
     2941 tgaaaaaaga tcaggtagga aagagcccaa caaaacaaaa aaatcgtggt aacccgtctg
     3001 agataaaacc tgcgttccag ttcttcatac agattataga aaaatcgact taaaaaagtc
     3061 ttctcgaaac ccaggtggct cgcccattca aggaacttca aactatgatc ttcatagttg
     3121 acccgtttac gaggcgcgtc tgtttttttt ggtccaaatt taggcaaagg catcagcttt
     3181 atggttcccg ttttattata caaacttttc gggtttttta tcgaaatccg cagtcttata
     3241 tgctaattct cggggtaatc tctttctttg tcgaggtcac ccaatatgcc aaaaggtcca
     3301 gagaatctca aagacattcg tgaatgtatg caggtgttaa tgaatattgt aacagacctc
     3361 cgcggaccca acggctgtcc ttgggataaa gaacagactc ataacacttt ggcgcgatac
     3421 gccatcgaag aaaccttcga aatggtcgag gccctggaag aacgagagga ctgccgacga
     3481 aagttgggcc aggcaccttc aaaggaagaa gagaataaaa accgaaactc atttgtttcc
     3541 ctcagcacca agtttaaaga ggaactcggc gacgttctct ttcaagtcgt tttacattct
     3601 caattagcca gcgaagaagg ctcctttgac ttcagcgatg ttgtgatttc tatttgtgaa
     3661 aagcttgtgc gtcgtcaccc tcatgttttc ggcgatgtca tagtcaaaaa ctccgaggat
     3721 gtgattaaaa attggaataa tattaaaaaa gaagaaaaag cgaagcgtgg tgaaaaggcc
     3781 tctctcattt cagtgcctcc ggggctcccc gctttacaaa gagcttttac cattggcgaa
     3841 aaaactcaca gaataaattt tgattggcaa ggcccgcaag aagtttggct caaagttgag
     3901 gaagaaatcg cggagttgca agaagccata gataacgacg tcatgagcga aattgagcat
     3961 gagcttggcg atgtcctttt tagcctggca caactcgccc gtcactatga actcgatcct
     4021 gaacaagtct tacgcgcggc caacacaaga ttcctcggtc gcttcgaaaa aatgattggg
     4081 gaataccgca aggctaaagc caacacctcc gatgtcgatg aggttttgaa aaaatttggc
     4141 gccctttcaa cggaagaaaa agaaaacttc tgggaactgg caaaacggaa tttcggaggt
     4201 taaaatgcgc caatatcatg aacttttaaa acatgtcttg gtccacggag tgcaaaaaga
     4261 agatcgcaca ggcactggca cactcagtgt cttcggttat caaatgcgtt tcaatctgga
     4321 agagagcttt cctcttctca ctacaaaaaa acttttcacc cgcgcgattt tccatgaact
     4381 cctctggttc ctcaaaggtg atacgaatat caagtatctt cgtgataaca aagtgaccat
     4441 ctgggacgaa tgggcggatg ccgagggcaa cttaggtcct gtctatggca agcaatggcg
     4501 ctcgtgggaa gctccagacg gacgcaaaat tgatcagatc agcaacgtcg tcgaacaaat
     4561 caaaaaaaat ccagactctc gccgtctgct ggtgatcgcc tttaatccag ccgacgtgga
     4621 taaaatggct ttgcctcctt gccacgcttt cttccaattc tatgtggcca acggaaaact
     4681 ctcttgccaa ctttaccagc gtagtgcgga tattttctta ggcgttccct ttaacatcgc
     4741 cagctatgcc ttgctggttc atatgatggc tcaggtttgt aatttaggcg tgggcgaatt
     4801 cattcatact ttgggtgatg cccatattta ttcgaatcac ctcgagcaag tgaaagctca
     4861 actgcaacgc gaattccgcc ctctgccgaa attaagcttg aatccagccg tgaaaaatat
     4921 ttttgatttc aaattcgaag acattcaact cacgggctat gatccgcatc cagccatcaa
     4981 aggagaggtc gcagtatgat tttaactcat gtcaccgcca tgtcgcaaaa tcatgtcatt
     5041 ggcactcaaa acaaactgcc ctggagcat
//
LOCUS       DATBTE010000112         3523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1039231, whole genome shotgun sequence.
ACCESSION   DATBTE010000112 DATBTE010000000
VERSION     DATBTE010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039231"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..1058
                     /locus_tag="VIG33_04130"
     CDS             246..1058
                     /locus_tag="VIG33_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8270050.1"
                     /translation="MAKTICIANQKGGVGKTTTSVNLSSALATLGKRVLLIDMDPQGN
                     ASSGLGIKRYESQDNNIYHILIGEREIQETIQKTKDPNLNVITANPDLVGAEIELVDM
                     PQREYRLKSALHTIGTLYDYILIDCPPSLGLLTLNALTAADSFLVPLQCEYYALEGLS
                     QLLNTAGLIKKNLNPQLHIEGIVLTMFDTRNNLSHQVVTEIKNHFGDKVFNAIVPRNV
                     RLSEAPSHGQSIFEYDPKSIGATKYFDLAKEVEQRTQAKIAKQTPQEEITNV"
     gene            1051..2097
                     /locus_tag="VIG33_04135"
     CDS             1051..2097
                     /locus_tag="VIG33_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY8270051.1"
                     /translation="MSELNSESSNKKKGLGRGLGSLLSGGPTAEGMASKTMVEGLDNQ
                     IAAIPAKTAQNSISRPIANSAPNQATAAPVAPISTEGKVWQVAIDKLSSGQYQPRKAF
                     EKESLLQLSQSIKSNGILQPIIARKVAGGRLEIVAGERRWRAAQSAGLHEVPVLIKNF
                     DDQETLELAIIENIQREDLNPIEEAEGYARLITEFKMSQQQVADKVGRDRATVANAVR
                     LLSLPGEAKKLIVDGNLSVGHAKVLLSLTDTRRQIDLAKKVISEKLSVRKLEKLVADA
                     QNLSSSTTTSQSDVQHSVTQRLISGLSDELQKILGTKVNIDYANSKGKIVVHFYSDDE
                     LTQIADRLKQGWQK"
     gene            2085..2450
                     /locus_tag="VIG33_04140"
     CDS             2085..2450
                     /locus_tag="VIG33_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY8270052.1"
                     /translation="MAEIKALVGGDDFLTGHVTSILDYGTRFEGKLSFEGTVQIGGEF
                     IGEIFTNDTVVINNTANIQAQVEADTIIISGRVEGNLFARRRVIMHPPAVFKGTVTSP
                     SLRIDEGVVFEGASYMPKS"
     gene            2557..2991
                     /locus_tag="VIG33_04145"
     CDS             2557..2991
                     /locus_tag="VIG33_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEY8270053.1"
                     /translation="MEIIHQLGINGTVFIQFGIFICAFFFLNLYVFTPYYKALEEREK
                     RTLGGEDLAQEFQKKSTELHSEYQTKAKEVTKKIKDIYDSHRLDALKEFDSLVSKARS
                     EAHTLLEANNRQIIQSIQATSAALRAETTSVSVAITQKLLGK"
     gene            3002..>3523
                     /locus_tag="VIG33_04150"
     CDS             3002..>3523
                     /locus_tag="VIG33_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEY8270054.1"
                     /translation="MKKWFPFLLVLGASAVFAAGHGEHEAGAIEVPRVVLYQVINVVI
                     LFGGLVYFLKGTIVKFYADRKANYLAAARKSQAARTEAEKQFIDIKHKLDQLQSSEDE
                     NISRAKAEATDLRHSLIKEAHEMALRIKTEAEQTAKIEIQKAQTHLREQLLKNSLEAA
                     KAVLVKDIGSADHQ"
BASE COUNT         1141 a          668 c          766 g          948 t
ORIGIN      
        1 ctgcttcagc gtctctttga gctgtttgag cttgagattc caagtcctga tatatttggt
       61 ctgccatttc tggatttgga ttaggtttat cgcagccaaa caggcctatt aaaaggaata
      121 aaaataaaat tcgcatattc acccgtgttc cacgtagaac atcggaaatt ctagataaaa
      181 cattaggaaa aacttgccat gttgaataaa atacaggatt tttaacacat catggaggaa
      241 tgaatatggc taagacaatt tgtatagcga accaaaaagg tggagttggt aaaactacta
      301 cttctgttaa tctctcatcc gcactggcta cactagggaa gcgggtccta cttattgata
      361 tggatccgca aggaaatgcc tcgagtggac ttggaatcaa acgttatgaa agccaagaca
      421 ataatattta tcacatatta ataggcgaac gtgaaattca ggagactatc caaaaaacta
      481 aagatccgaa tctcaatgtt atcactgcaa atccagattt agtcggagca gaaattgaac
      541 ttgttgacat gccgcagcgc gaatacaggt taaaatctgc gcttcatact attggtaccc
      601 tttatgatta cattttaata gattgtccgc catctttagg attgctgacg ttgaatgcct
      661 taacggcagc tgacagtttt ttggtgccac tgcaatgtga gtattatgcg ctcgaaggac
      721 tgagccaact tctgaatact gcaggtttaa ttaaaaaaaa tcttaatcct caactacata
      781 tagaaggcat tgttttaaca atgtttgata caagaaataa tctcagccac caagttgtaa
      841 cagagattaa aaaccatttc ggcgataaag tttttaatgc gattgttccc agaaatgttc
      901 gtttaagtga agccccaagc catggacaat ctatttttga atatgacccc aaatcaatag
      961 gcgcaactaa atactttgac cttgctaaag aagttgaaca gagaacgcaa gcgaagatag
     1021 caaaacaaac tccgcaagag gagataacaa atgtctgaat taaatagtga aagttcaaac
     1081 aagaaaaaag gtctaggacg tggtctagga tctttgttga gtggaggtcc aacagccgaa
     1141 gggatggcaa gcaagactat ggttgaaggc ttggacaatc aaatagcagc cattccggca
     1201 aaaacagcac agaattctat tagtagaccg attgcgaact ctgctccaaa tcaagctaca
     1261 gcagcaccgg tggctcctat aagcaccgaa ggtaaagtat ggcaggtagc gatcgataaa
     1321 ttgtcttcag ggcagtacca acctcgtaaa gcctttgaga aagaaagcct tttgcagctt
     1381 tctcaatcta ttaaatcaaa tggaattctt cagccgatca tcgctagaaa agttgcggga
     1441 ggacgtcttg aaatcgtagc tggagagcgc cgctggagag ctgctcagag tgctggactg
     1501 catgaagtgc ctgttttaat taagaacttt gacgaccaag aaactcttga gcttgcaatc
     1561 atcgaaaata ttcagcgtga ggatctcaat ccaattgaag aagctgaagg ttacgctcgg
     1621 cttattacag aatttaaaat gtctcagcag caagtagcgg ataaagttgg ccgcgaccgt
     1681 gcgacggtag caaatgccgt tcgtttatta tcccttcctg gggaagcaaa aaaattaatc
     1741 gtagatggca atctttcagt aggacatgca aaagttcttt tatctttaac agatacgaga
     1801 agacaaattg acctcgcaaa aaaggtgatt agcgagaagc tttctgttcg taaactagaa
     1861 aagctagtag ctgatgctca aaatctaagt tcttcgacaa caacgtctca atcagatgta
     1921 cagcatagtg taacgcagag gctcatttct ggcctctctg atgagcttca aaagatccta
     1981 ggaaccaagg tgaatattga ttatgctaat tccaagggga aaattgtagt tcatttctat
     2041 tcggatgatg agctcactca aatcgcagat aggttgaaac aaggatggca gaaataaaag
     2101 ctcttgtagg cggtgatgat tttttaacgg gtcatgtaac ctctatattg gattatggga
     2161 ctcgttttga agggaagttg agttttgagg gtacggtgca aattggtgga gagttcattg
     2221 gtgaaatctt tactaatgat acagtagtca tcaataacac cgccaacatt caggcccaag
     2281 tagaggccga caccatcatc ataagtggac gggtagaggg aaatctcttt gcccgacgca
     2341 gagttataat gcaccccccc gcagttttta aaggtactgt tacgtcgcca agcctacgaa
     2401 tagatgaagg tgtggtattt gaaggtgcct cttacatgcc caagtcataa ccaaagtcct
     2461 ttgtttacgg tgatttaaag gtactttgac cttgaccgtt tctttccaaa aatgctaatt
     2521 ccgaaggttc aaaaaaccag tagggatttc aatacaatgg aaattatcca ccaactagga
     2581 attaacggta ctgtatttat tcaattcggt atcttcattt gtgcattctt ctttttgaat
     2641 ttgtacgtct ttacgccata ttacaaggct cttgaggagc gtgaaaagcg cactttgggt
     2701 ggcgaagatt tggctcaaga attccaaaaa aaatctaccg agcttcattc ggaatatcag
     2761 acgaaagcca aagaagtgac aaagaaaatt aaagatatct acgattctca tcgtttagat
     2821 gcgttgaaag aatttgattc tttagtaagt aaggcgcgct cagaagcgca caccctctta
     2881 gaggcaaata atcgacagat tatacaatcc attcaagcaa cctctgcagc tttgagagct
     2941 gaaacgacaa gtgtgtctgt ggcgattact caaaagcttt taggaaagta agagagaaaa
     3001 tatgaaaaaa tggtttccat ttcttttagt acttggagca agtgctgtct tcgctgctgg
     3061 ccatggtgag catgaggcag gggcgatcga agttcctaga gttgtacttt atcaagtcat
     3121 taacgtagtt attttatttg gtggtctcgt ttatttttta aaaggcacca ttgtaaaatt
     3181 ttatgccgac cgtaaggcga actacttagc agctgcacgt aaatcacaag cggcacgcac
     3241 agaggcggaa aagcaattta ttgacattaa gcataaactc gatcaattgc aaagctctga
     3301 agacgaaaac atttctcgtg caaaagcgga agcaacagat ttacgtcatt ccttgattaa
     3361 agaagctcat gaaatggcgc taaggattaa aactgaagct gagcaaacgg caaagattga
     3421 aattcaaaaa gcgcaaacgc atcttcgtga gcagcttttg aagaattcat tggaagctgc
     3481 aaaagctgtg ctggtcaaag acataggctc tgcagatcac caa
//
LOCUS       DATBTE010000113         4419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_14601, whole genome shotgun sequence.
ACCESSION   DATBTE010000113 DATBTE010000000
VERSION     DATBTE010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14601"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VIG33_04155"
     CDS             complement(<1..432)
                     /locus_tag="VIG33_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270055.1"
                     /translation="MRKFLLNFGGLFLCIFGTLSCSRLDIAVNWADTYIVSQVDDYFD
                     LTSQQNKELKESLKKDILKIRQEQFLDWARELRRFNKYILEKNLNEETFHKYFAKALD
                     TTRKLQSYFANTAVKFISTTSPAQLEHFEMTVRKKNVEDQKK"
     gene            complement(429..1010)
                     /locus_tag="VIG33_04160"
     CDS             complement(429..1010)
                     /locus_tag="VIG33_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HEY8270056.1"
                     /translation="MKPANGNKQAALSFFFGGLIPLIAFTVIEENFGVITGLIAGMVF
                     GLGEILYEWIRHRKVATMTWVGNGLLLVLGGISLISSEGLWFKLQPAFMEGPFALAMW
                     GSCLLKKPLLVTLAEKQGQSLPELVKRKLNGVTWRAGLFFALHTALAVWAALEWSTAN
                     WALLKGIGLTLSFILYLGAEVFYLRRSMANIKE"
     gene            complement(997..2346)
                     /locus_tag="VIG33_04165"
     CDS             complement(997..2346)
                     /locus_tag="VIG33_04165"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY8270057.1"
                     /translation="MTKTSFDLILKNATVLVPDATGIGLIEEKLDVAVANKRIVQIAS
                     GLTNPAAKTLDLRGLHLLPGVIDSQVHFREPGLTHKEDLETGTRSAILGGVTSVFEMP
                     NTSPSTTTQEAFEDKLHRAKNRCHAHYAFFIGASAENANELARLETLPHCSGIKVFMG
                     SSTGTLLVDNDEALEKILRSGHRRVIVHSEDEARMRERRSLAVDSQDVHMHPVWRDVE
                     SAMISTQRLINFARKTGRKVHVLHVSTGEEMEFLQNHKDIASVEVLPQHLTLFAPDCY
                     DRLKNFAQQNPPLREKHHLERIWKAVLDGTVDVMGSDHAPHTREEKNRPYPQSPSGMP
                     GVQTLVPIMLNHVNNRRLALPQFVKLVSEGPRRVFGIKNKGRIEVGYDADFTVVDMKK
                     IMTIENKMMASRSGWTPYDGMKVQGWPTHTILMGEVVMENDTVILPHQGQGVFFETR"
     gene            2492..3328
                     /locus_tag="VIG33_04170"
     CDS             2492..3328
                     /locus_tag="VIG33_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270058.1"
                     /translation="MEKFLEFWSHYNAAIIESLVALILVSAIYLAYRSLFAAKEVETS
                     HEVISGGAFNAVQIEKTLQKILDQQSSRPASLTSGVATETEVPAEILQELDFLKKALE
                     EKEKQIATLQAKAAQVNAGPSDEQIAAAASAAAGLAEKERLDYVSKIKELEARLAEYE
                     IISEDIADLSFFKEENTRLTKELEEARGEKPPLQSAAPEPEPSLESVVPPKKMTVPES
                     EVLPEAEVFAEAAGSIDDSLMAEFAKAVEDQKSGAAPAADTKEDENRKLVAEFENFNK
                     KS"
     gene            3330..4064
                     /locus_tag="VIG33_04175"
     CDS             3330..4064
                     /locus_tag="VIG33_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY8270059.1"
                     /translation="MKKMNLISAFLVLTPFSGVRAAIPPKNPLSPRVMEILNLPPENR
                     TQAVLSTTEDMYHDFLGVAFSEKQSMRLRWRALMLAAENRRGKATPDLLKAANHKQWF
                     MRNAALVALAEVNDAEAQKLAKKLVKDKALVVRSAAVEILQKNVRPEVRDLLWEEMAQ
                     KYNFRNKESLWIRSQIVEALAQKPADHELKTFSKFLYDKDSRVQIAAVAGMEKLTGVK
                     LGEAQSSREKLVLLWQDYARKEKIGL"
     gene            complement(4059..>4419)
                     /locus_tag="VIG33_04180"
     CDS             complement(4059..>4419)
                     /locus_tag="VIG33_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HEY8270060.1"
                     /translation="LLSHRKLASLNDADLLQTVEKFSELPGVVKREPTLLSLTLKALR
                     NDFAIMASYQHRYSQQKLSLPSLIFGGTTDPHVSLQDLQGWSSLVNIKKNPLLFSGDH
                     FYIFSQLDKILGELFIL"
BASE COUNT         1232 a          960 c         1066 g         1161 t
ORIGIN      
        1 cttcttttga tcctctacgt tcttttttcg gacggtcatt tcaaaatgct ctagctgagc
       61 cgggctggtt gtggaaataa atttcacggc tgtgttcgcg aaataggatt gtagctttcg
      121 ggtggtgtcc aaggcttttg caaagtattt gtgaaaggtt tcttcgttca aatttttttc
      181 tagaatatat ttattaaaac ggcgaagttc tctcgcccaa tcaagaaatt gttcctggcg
      241 gatcttgaga atatctttct tgagagactc ttttagttct ttgttttgct gagaggtgag
      301 atcaaaataa tcatcgactt gtgaaacgat ataagtatcc gcccaattga cggcgatatc
      361 gagacgactg caactcaggg ttccaaaaat acaaagaaaa agtccgccaa aatttaatag
      421 aaattttctc attcttttat attagccata gatcgccgca gataaaagac ctcagcccct
      481 aaataaagaa taaaactcag agtgagtcct atgcctttta acaaggccca attcgccgtg
      541 ctccactcca gggcagccca cacggccaaa gccgtatgaa gggcaaagaa aagccccgca
      601 cgccaggtca cgccgttgag tttgcgcttt acgagttccg gtaacgactg tccttgtttt
      661 tcggcaaggg ttactaagag gggctttttg agaaggcatg aaccccacat agcgagagca
      721 aaaggccctt ccatgaaagc gggttggagt ttaaaccaca gtccttcact ggaaattaaa
      781 gagattccac ccagaaccaa taacaggcca ttcccgaccc aggtcatggt ggcgactttg
      841 cggtggcgaa tccattcata aaggatttca cctaaaccaa acaccatgcc agcgataaga
      901 ccggtgatca ctccaaaatt ttcttcaatg accgtgaagg caatgagcgg gatcaggcct
      961 ccaaagaaga aagaaagagc cgcttgcttg ttgccgttag cgggtttcaa aaaaaactcc
     1021 ctgtccttga tgaggcaaga tgacagtgtc attttccatg accacttcac ccattaaaat
     1081 ggtatgagta ggccatccct gaactttcat tccatcataa ggtgtccaac cgctgcgact
     1141 ggccatcatt ttattttcaa tggtcatgat ttttttcata tctacgacag tgaagtcggc
     1201 atcatacccc acttcaatac gccctttatt tttaattccg aaaactctgc gcgggccttc
     1261 acttactagc tttacaaatt gaggaagagc aagacgtcga ttattcacat gattcaacat
     1321 aatcgggacc aaagtctgca caccgggcat tcctgaagga ctttgcggat agggacggtt
     1381 tttttcttca cgagtgtggg gagcatgatc ggagcccatg acatccactg tgccatccag
     1441 aaccgccttc caaattcttt cgagatgatg tttttcccga aggggcggat tttgttgcgc
     1501 aaagttttta agtcggtcgt aacaatccgg ggcaaagaga gttaaatgct gtggcagaac
     1561 ctccacgctg gcaatatctt tgtgattctg taagaactcc atctcctcac ctgtggagac
     1621 gtggagaaca tgaactttgc gtccagtttt tcgagcaaag ttaatcaggc gttgagtgga
     1681 aatcatggcg gactccacat cgcgccatac aggatgcata tgcacgtcct gagaatccac
     1741 ggcgagagag cgtcgttcgc gcatgcgggc ttcatcttct gagtgaacaa tgacccgacg
     1801 atgtcctgaa cgcagaattt tttcgagagc ttcatcgttg tccactaaga gagtacccgt
     1861 cgagctgccc atgaagactt ttatacccga gcagtggggg agcgtttcta gccttgccag
     1921 ctcgttggca ttttccgcag aagctccaat gaagaaagcg taatgggcat gacagcgatt
     1981 cttggctcgg tgaagtttgt cctcaaaagc ctcttgggtg gtggtggaag gagaggtgtt
     2041 cggcatttca aaaacactgg tgactcctcc taaaatagca gagcgtgtgc cagtttctag
     2101 atcttcctta tgggtgagtc caggttcgcg aaaatgcact tgggaatcga tcacgccggg
     2161 taaaaggtga agtccccgga ggtctagagt ttttgccgct ggattggtaa gtccagaagc
     2221 tatttgcacg attcgtttat tcgcgaccgc gacatcaagt ttttcttcga tgagccctat
     2281 tccggttgca tccggaacaa gaacggtggc atttttgaga attaaatcaa aagaagtttt
     2341 tgtcataggt tacctgctta gattccaatt tatatttttc aaagtaacat gtttgccctt
     2401 ctttggtcca gacggaaagg aagtggtttt cccagccttc ctagcttgcc atcttgacct
     2461 tgtgagcgga cgcagcgaga attggctaag catggaaaaa tttttagaat tttggagtca
     2521 ctataatgca gccatcattg aaagtttggt ggcattgatc ttggtttccg cgatttatct
     2581 cgcctatcgg agcctttttg cggctaaaga agtggagacc tcccatgagg tgataagtgg
     2641 cggagctttc aatgccgtgc aaatcgaaaa aactttgcag aaaattttag atcaacagtc
     2701 ttctcgcccg gcctcgttga cttcaggggt ggcaactgag actgaggttc cagctgagat
     2761 cttgcaagag cttgattttc tgaagaaagc tcttgaggaa aaagagaagc agattgcgac
     2821 tcttcaagcg aaagcggcac aagtcaatgc cggacccagt gacgagcaaa tcgcggctgc
     2881 ggcttcggcc gctgcgggat tggctgaaaa agaacggctt gattatgtat caaaaattaa
     2941 agaactcgaa gctcgtttgg ctgagtatga aattatcagc gaggacattg ccgacctctc
     3001 tttctttaaa gaagaaaata ctcgtttaac gaaagaactg gaagaggccc gaggcgaaaa
     3061 gccacctcta cagtcggccg caccagagcc tgaaccgtca ttggaatcag tggtcccacc
     3121 taaaaaaatg acggtgccgg aatcagaagt attgccagaa gctgaagtgt ttgcggaggc
     3181 agccggttcc attgatgatt ctttaatggc tgagtttgca aaagcagtgg aggatcaaaa
     3241 gtctggtgct gccccagcgg ccgacactaa ggaagatgaa aatcgtaagc tcgtcgctga
     3301 atttgaaaac ttcaacaaaa agagttgata tgaaaaagat gaatttgata agcgcttttt
     3361 tagtcttaac tccattcagt ggggtgcggg ccgctattcc cccaaaaaat ccactcagtc
     3421 ctcgtgtgat ggagatattg aatcttcctc cagagaatcg gacccaagcc gttttatcga
     3481 ccactgaaga tatgtatcat gactttttgg gtgtcgcttt ttccgaaaaa caatcgatgc
     3541 gtcttcgctg gcgagccctt atgcttgcag ctgaaaatcg tcgaggcaaa gccactccgg
     3601 atcttttgaa ggcagcgaat cataaacaat ggtttatgag aaatgcggcg ctggtggcac
     3661 tggcagaagt taacgatgct gaagctcaaa aattggcaaa aaaactggta aaagataaag
     3721 ctttagtcgt gcgatcggcg gcggtggaga ttctacaaaa aaatgttcgt cctgaagtgc
     3781 gtgacttgct ttgggaagaa atggctcaga agtacaattt ccgcaataaa gaaagtcttt
     3841 ggatccgctc gcaaattgta gaagccttgg ctcaaaaacc agccgatcat gaattaaaaa
     3901 cgttcagtaa atttctttat gataaagaca gccgtgtaca aatagctgcg gttgcgggaa
     3961 tggaaaaact gaccggtgtg aagttgggcg aggcccagtc ttctcgcgaa aaattagtct
     4021 tgttatggca agactacgct cgcaaagaaa aaattggcct atagaataaa cagttccccg
     4081 aggattttat caagctgaga aaaaatataa aaatgatctc cgctgaaaag caggggattt
     4141 tttttaatat tcactaaaga actccagccc tgcaaatctt gcaaagacac gtgggggtcc
     4201 gttgtgccac caaagattaa gctgggtaaa ctgagttttt gctgggaata gcgatgttga
     4261 taactggcca taattgcgaa atcattgcgc aaggctttca gagtcaggct caaaagcgtg
     4321 ggttcgcgct tgacgactcc aggaagctct gagaattttt ctacggtttg caaaagatcc
     4381 gcatcgttga gcgatgccag ctttcggtga cttaaaaga
//
LOCUS       DATBTE010000114         2962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_219529, whole genome shotgun sequence.
ACCESSION   DATBTE010000114 DATBTE010000000
VERSION     DATBTE010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219529"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VIG33_04185"
     CDS             <1..802
                     /locus_tag="VIG33_04185"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY8270061.1"
                     /translation="AAVDRSTGEIDWAGTQVYVPNKFVLSGIKKKNSGLLYYGASINP
                     YRKDAIERLAQAKMDGAVLIKWIPSTMNIDPSDPSLEPFYRKLVELRLPLLVHTGREQ
                     TFTDAKDQLCDPFLLKFPLDLGVTIIAAHLATTGENEGEPDYKRLHKLFEIESYKSHL
                     FADISATTQYNRVKGFFSLIHDPLFHGRLVNGTDWPLIEAEFLGMKLVPYKLYSSPLF
                     GSILSDAQVFELDSISNGFDRDIRLKEFLGTPEDLFYHAENLLNISLP"
     gene            complement(894..1076)
                     /locus_tag="VIG33_04190"
     CDS             complement(894..1076)
                     /locus_tag="VIG33_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEY8270062.1"
                     /translation="MAKKGGESVPPEERSFSKDHELAREAGKKGGESVPPEERSFSKD
                     PNLAREAGKKGGKSSH"
     gene            complement(1198..1380)
                     /locus_tag="VIG33_04195"
     CDS             complement(1198..1380)
                     /locus_tag="VIG33_04195"
                     /inference="COORDINATES: protein motif:HMM:NF022150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEY8270063.1"
                     /translation="MATKQGNTSDNKGFASMDPQKQKESAKKGGEASHGGGRDSSSSK
                     SDKSEAGRKGGSQSGK"
     gene            complement(1681..1947)
                     /locus_tag="VIG33_04200"
     CDS             complement(1681..1947)
                     /locus_tag="VIG33_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY8270064.1"
                     /translation="MGAELAKEPGTWQAWAWIKWKPGASETAWEEWRKNGTVIGAWST
                     SGEWDCVLALNISNPNDLEQFVWKKIRQNPWVESTSTTWAKQCW"
     gene            2297..>2962
                     /locus_tag="VIG33_04205"
     CDS             2297..>2962
                     /locus_tag="VIG33_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270065.1"
                     /translation="MVYRLFNKSIAALCLAMLALSFQNCDKGFKVLDDFTSFSSTTSL
                     GSAMNCSPTFAPERVPLRLLSNLEYDFIAADVLLSKRKPSADAKFDIPALGSSGFSNT
                     SMTSEASTPTISDMTMEKYWTAAGALADEVIANKAQSGSAYSSLAACALNVSTVSETC
                     YASIARSLALKLWRRPLSETAPNNELSRLVALLKGGASFEGGFHDLIKALLISPYFLV
                     VSFN"
BASE COUNT          783 a          700 c          567 g          912 t
ORIGIN      
        1 cgcggcggtg gatcgatcca ccggagaaat tgactgggcg ggaactcagg tctatgtccc
       61 gaacaaattt gttttgtcag gaattaaaaa gaaaaactca ggtcttttgt attatggagc
      121 cagcatcaac ccctatcgta aagatgccat tgaaagattg gcacaagcga aaatggatgg
      181 ggccgttctc atcaaatgga ttccttcgac aatgaatatc gatcccagcg atccttcgct
      241 tgaaccattc tatagaaaac tcgtggaact tcgccttccg cttttagttc acacgggacg
      301 cgagcaaacc ttcacggatg ctaaagatca actctgcgat ccttttttat taaaattccc
      361 ccttgattta ggcgtcacta ttatcgccgc tcacttggct acaaccggcg agaatgaggg
      421 cgaaccagat tataaaagac tccacaaatt atttgaaatt gaaagttaca aaagtcattt
      481 atttgcggat atttcagcca cgacccaata caatcgggtt aaaggatttt ttagtttgat
      541 ccacgaccct ttgtttcatg gacgacttgt caatggaact gattggcctt tgattgaagc
      601 ggaatttcta ggtatgaagc tcgtccctta caaactctat tcttcaccct tattcgggtc
      661 catcttaagc gatgctcagg tgtttgaact cgattcgatt tccaatggct ttgatcgtga
      721 tattcgccta aaagagtttt tagggactcc ggaagatctc ttttaccatg cagagaatct
      781 ccttaacatt tcccttccgt gaaaagagaa tagacctatt actgaaacct cggctcaatg
      841 ataggtctat tctttaattc tacttaaatc tctacttaac taagtttcaa tttttagtgg
      901 gagctttttc cacctttttt accggcttct cgagctaaat tgggatcttt agagaaagac
      961 ctttcttccg gaggaacact ttcgccgcct tttttacccg cttctcgagc taattcatga
     1021 tccttcgaaa aagacctttc ttcaggagga acgctttccc cacctttttt tgcaatctcc
     1081 ttttgcttct ctggatccat agaagcaaat ccgcgattgc tagtgttttt attttgatct
     1141 tgtgattgat tggtagccat aaacacctcc cattaattaa tcacggttag tgaaaaatta
     1201 ttttccactc tgcgagccac ccttacgtcc agcttcagac ttgtctgact tactcgagct
     1261 cgagtcacgt cctcctccgt gggaagcttc gcctcccttt tttgcacttt ctttttgctt
     1321 ttgaggatcc attgaagcga atcccttatt atcacttgta tttccttgtt tcgttgccat
     1381 tatgacctcc ttgaggtggg tgcaatcttt tgagatgatt atacgctgag tgaagtttta
     1441 gatctaaatg atctttaaca tttaacgagc ctcatatcac ttgtggtgag gcctcacaga
     1501 aataattcaa aggaaaaaat aataaaaaat ataatattga gaagactcat cttatttgat
     1561 cgttaataag cgatttcttt gaaatttcac gtaatggaaa tgatattgga tgacatcaat
     1621 tttatttagg aaataaatgg tgatagttaa gaactatcac catccgctat tgccttcagc
     1681 ctaccagcac tgttttgccc atgttgttga agtgctttca acccacggat tctgacgaat
     1741 ttttttccat acgaactgct cgaggtcgtt gggatttgaa atgttgagcg cgagaacaca
     1801 gtcccactct cctgatgttg accaggcacc tatcactgtt ccgtttttac gccattcttc
     1861 ccaagccgtt tcactagcac ctggtttcca tttaatccag gcccaagctt gccatgttcc
     1921 tggttctttc gccaactctg ctcccataaa atctccttat gattgattca gccttagaat
     1981 aaacaacttt cactacaata ccgaagaaaa tttaaaattc agaattcaaa agaacaaaaa
     2041 tagacaaaag accagaactt taaatctcat tttgagaaag gaatcgcacc aagacaaatt
     2101 accttggatt tagaaaacaa ataataaatt atgctttaaa aaagacctct cctcgttcat
     2161 tctcatattt atgcacattt ataatgtctc aatcagagcc ctagccacgt cagctccacc
     2221 tcacaacctc aaccgggata taggtttgtc ttagggatca tgaagtcgct gtgcaggtat
     2281 tcaaatgggg gaatgcatgg tgtatcgact tttcaacaag tctatagctg ctttatgtct
     2341 tgcaatgctt gccttatctt ttcaaaactg cgacaaaggc ttcaaagtcc tcgacgattt
     2401 tacctcgttt tcttcaacaa ccagcttagg ctcagctatg aactgcagtc ccacttttgc
     2461 tcctgagcgg gtgccgctgc gtttgctgtc caacttagag tatgatttta tcgccgccga
     2521 tgtgcttctc tctaaaagaa aacccagcgc cgatgccaaa tttgatattc cggcgctggg
     2581 ctcttcaggc ttttccaata ccagcatgac cagtgaggcc agcacgccga ccatttctga
     2641 tatgaccatg gaaaaatact ggaccgcggc cggcgccctg gccgatgaag tgattgccaa
     2701 taaagcccaa agcggcagcg cctactcctc tttggctgcg tgtgccttga atgtttccac
     2761 ggtctcggag acgtgttatg cctccattgc ccgaagcctc gcgcttaagc tctggcggcg
     2821 tcccttgtct gaaacggccc ccaataatga actcagccgg cttgtggccc tcttaaaagg
     2881 tggcgccagc tttgaggggg gatttcatga tcttatcaaa gctttattaa taagccctta
     2941 tttcttagta gtatccttta ac
//
LOCUS       DATBTE010000115         9878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1995390, whole genome shotgun sequence.
ACCESSION   DATBTE010000115 DATBTE010000000
VERSION     DATBTE010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 9878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9878
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1995390"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..549)
                     /locus_tag="VIG33_04210"
     CDS             complement(163..549)
                     /locus_tag="VIG33_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270066.1"
                     /translation="MKKFVFISFSMVGLGILLFIFVNWYSYIFSVRIDGEIYGVERVE
                     APMAVLTTEGAKPAPQVFSYAVAVRDSKTGEIFTASSEDRRWAVVQKGQCAEVKFLPY
                     PPWSLTRSGSYFGARLIRLYDCPKKP"
     gene            complement(583..2061)
                     /locus_tag="VIG33_04215"
     CDS             complement(583..2061)
                     /locus_tag="VIG33_04215"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8270067.1"
                     /translation="MQEKLAAAKFREAEAIEEMRSIVHVICHWMMVPLFLVFWLADCI
                     LYPEYKWPFLGIRLLVIPLCLGINFLIDRTRSFNQTQFVAILALGVGAAGFINAIILM
                     VPDAATPYYAGLNLVALGGLSFVPFSRRFFALLAIGIFLPYYIIVLLKARGIEDYKAI
                     FVFSFFIVGSLVLCFLIRHFNGALRSREINSRLALKTEISNRDLIIQEKTGEAVRLNV
                     LSSQFSPQVVRAIREGRVDLDKGVHRSQICAIFVDIVGSTERVVRLDQRKVDMVLARF
                     MDTVVSTFLKYDLTIDKFQGDGILAFSNDPIPHADFVQRTCYAALEVRELLQADREFY
                     LMNWKKEMQIRIGISVGYANVGFYGNKKFFRCYTAIGAPLPLASRLTNLAEPDQILID
                     CDIAAILELENFQMTRLGEREIKGFEGDRNIVFELIQAPQDKMNAFAGSLTCPHHPDA
                     VLFLDNNDKGHYVMRCRHCDFSLDEYSMEILGQIPHMKVKAA"
     gene            complement(2113..3591)
                     /locus_tag="VIG33_04220"
     CDS             complement(2113..3591)
                     /locus_tag="VIG33_04220"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8270068.1"
                     /translation="MTRPSAAMKQEGFLINEEGTAKYKKIIRQDRLQLDPAKCFLDFH
                     GTRIEVLNISTFGCATIVSAEDSLILAKLYQEKEYCTAKVYFEGTETQTLHLRKVREE
                     SHPTSVTNERIWAFETVGEPFRVERIKVLELTSAVLQKQNEYGAKIERLPPPYRQIVF
                     QVRDWFQKLKESIDQLEKDAPVDNAQEAQEYRMTIVENIGEYIGNVIPQVYKQVPPML
                     KNLSPEDAQLATDFIREQLGPYIYGAPFAARAFHKPRGYAGDYEMMNHLYRAEVTGKT
                     LFDQCMHKYLVGEPAGVAVKNRGYYLVEKIKQIFEVHSSQEPLKIISVASGPAMEQQL
                     FLQNYKEFYGRPVSFTCLDQDEESLKYAQKHIGSIDRFVKSGFKFNFKNLAIRNVIAG
                     GLPEKDYALVYSAGLFDYFTEPVAQMAAQKMLACIKPGGKLIIGNFSTDNPTTPLMEL
                     CLDWNLIYRSKEDLERMFKGFGSKVYVEKEPLGVNLFVVIEK"
     gene            3790..5370
                     /locus_tag="VIG33_04225"
     CDS             3790..5370
                     /locus_tag="VIG33_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HEY8270069.1"
                     /translation="MLEAVLTGIGSLVVGGGLGFTLRNYINTYTLKKAKQEAADLIAE
                     ANDAVEIKALEEKERTQEIELEMWSKVEPDMLKVEARIEELQELIDEKKQKHDAALAE
                     ERKKLQIHENETQQQEKAVQAQEIALQKIRAAQKQLNDEFTKRLSQKLNTSPQETKQQ
                     IAETLEQETYQRLQKWAEEYEEETKEHAETRAKRILDVALDRFARPYCAERGIGAVNF
                     PDANARRLLCDAKGENIKTLQEVGGCDIIVEPEMEMVGVAGFDPVRRELTRRVIERVL
                     KEKKNLTPDFIRKVAENQKKELFRQIKADGDAIAKELRQQDLHPEIKQMMGSLRYRYS
                     FTQNQYFHCGEVGWLAGLLAAELKMNVKTARRSGLLHDIGKSMDHAIDGGHAVIGADF
                     IQARNESPEVVHAVRAHHYDEQPSTDHAFLVIAADAISGARPGARRSTIESYNQKVSE
                     LQDIARSFDGVTDCYVLNGGRECRVMVNSRKVDDSQTLALGRKIATKIEEECNYPGQI
                     KVVIVRETVVSESTKGHN"
     gene            complement(5446..7881)
                     /locus_tag="VIG33_04230"
     CDS             complement(5446..7881)
                     /locus_tag="VIG33_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY8270070.1"
                     /translation="MNSIHGLFLECPWWALLGSLVGLLAVGFLGSPLIIWTIVLAVIF
                     VGFKLPVWLLIVYAVVMVIFNVKPLRAALVTKGVMTLFEKLHFLPKISDTERAAIDAG
                     TVWVEKDLFSGKPEFSKLMQEPYPQLTAEEQAFMNGPVEELCKKIDHWEMWKSRKLSD
                     EAFAYIKKEKFLGMIIPKEYGGLGFSALAHSEVIMKLASRSLSTAVTVMVPNSLGPAE
                     LLVHYGTDEQKKYYLPRLARGEEIPCFGLTEPTAGSDAGSIISSGVVFKGTDGKLYIR
                     LNWNKRWITLAAISTCIGLAFRLRDPEGLLGMGEDIGITCGLIPSNTPGVVIGRRHDP
                     LGTPFYNCPTQGKDVVVPLEAVVGGKDGCGKGWLMLTECLAAGRGISLPAQATGGAKL
                     ASRVVSAHSVIRRQFGVSIGKFEGVEEPLARLGAATYLLEAMRKYTLGALDQGLKPSV
                     ITAIQKYYSTEMGRKGINDAMDIMGGAGISMGPRNLLAEIYIGTPIGITVEGANIMTR
                     TLIIFGQGALRAHPFAYAEVKAMDAKDLGAFDTAFWGHVGHVVRNLCRSIGLSLTRGY
                     LAPAPSAHPQMKRYFRRLSWASATYAIVADIAMAVLGGELKMREKITGRFADILGYMY
                     MATSILRRFEAEGRRDEDLAFAHYNLKLCMAEIQKGFDGLFDNLKIPGLRWFVKGWIG
                     SWSRINSLGSQASDGWSHAIASAMLQEGSVRDRLTQGIFLPTKEGEQLHRLENAFSIT
                     LRAEAAEKKIKKAIRDGLLPKKKVALLLDDARGKNVISEDEYKLIQQSDTVRYDAILV
                     DDFSEEQYHATHG"
     gene            complement(8004..8210)
                     /locus_tag="VIG33_04235"
     CDS             complement(8004..8210)
                     /locus_tag="VIG33_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HEY8270071.1"
                     /translation="MKLVVVAALLALSFTTGFTCSKNTPVETAPAPTPAAQEQMAAPA
                     EGQPAATATPAGEAPAATPGSETK"
     gene            complement(8332..9150)
                     /gene="mutM"
                     /locus_tag="VIG33_04240"
     CDS             complement(8332..9150)
                     /gene="mutM"
                     /locus_tag="VIG33_04240"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY8270072.1"
                     /translation="MPELPEVETVRNGLAAILKDHPVIEKIELKRKDLRDPIPVKKVQ
                     SLVGQRIVSVERRAKYLLLKTPAGSLLSHLGMTGTWRVAVLGEERDHDHIYLHLGGGL
                     RLAYRDPRRFGCFDFVALGQEENHAKLKILGYEPLTEAFSGDALWKKLRGKVTAIKVA
                     IMDQRIVVGVGNIYASEALFRAGIRPSIKAMKVSREKCERLARIIKEVLTEAIAAGGS
                     SISNYEQFSGESGYFQQSFRVYDRKGQKCVVCSTEISAQILGGRSSYWCSNCQK"
     gene            complement(9239..9718)
                     /locus_tag="VIG33_04245"
     CDS             complement(9239..9718)
                     /locus_tag="VIG33_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY8270073.1"
                     /translation="MVWCNSSKVFEYLKPFPKNKESRRVHIHSALKEIVERRLKMRAP
                     GCNYLFHVEGKPLNYCTIQSNYRWAQAKTGIPYTGTHCLRHGMATLARRVGGMGLDSV
                     MAMTGHKDLKLADHYSKIDGEVQKETSLKVLEHINQLGLSKEDRKLGDNVVPFRRVK"
BASE COUNT         2918 a         2466 c         2017 g         2477 t
ORIGIN      
        1 tctccgatga ataccaaaat tatttttaat tttcaaaaat tctttccacg cttgaaagac
       61 gtggatgcac aaatcatcgt cgatagcctg cagaaaagcg ctgaaatcaa ggccaacgga
      121 acaagtgttg agttaaagta attttttaaa tcattaagtt tactagggct ttttggggca
      181 gtcataaaga cgaataagcc tggccccaaa ataagaaccc gaacgagtaa gactccacgg
      241 tgggtacggc aaaaacttca cttccgcgca ttgtcccttt tgcaccacgg cccagcggcg
      301 atcctctgag gaggcggtga atatttcccc ggttttacta tcccttacag ccaccgcata
      361 agagaaaacc tgcggcgcgg gcttcgcccc ttcagtggta agaacggcca tgggagcttc
      421 cacacgctcc acaccataaa tttcgccatc aattcttaca gaaaaaatat aggagtacca
      481 attcacaaaa atgaaaagca aaatacccaa gccgaccatt gagaaagaaa taaatacaaa
      541 ctttttcata gaattgaatt tagcccttaa ccgcctcaat aatcaagcag ctttgacttt
      601 catatgcgga atctgaccta gaatttccat agaatattca tccaaagaaa aatcgcaatg
      661 acggcacctc atcacataat gacctttgtc gttgttatcc aaaaaaagca cggcatcggg
      721 atgatggggg caggtcagcg agcccgcaaa agcgttcatc ttatcctgcg gcgcctggat
      781 gagctcaaag acgatatttc gatcgccctc aaagccttta atctctcgct cgcctaaacg
      841 agtcatttga aaattttcca gctctaaaat ggccgcgata tcacagtcga taagaatttg
      901 atcaggctca gccagattcg tcagtcttga agctagaggc aaaggcgccc caatcgccgt
      961 ataacaacgg aaaaactttt tatttccata gaaacccaca ttggcatagc cgacggagat
     1021 cccaatacgg atctgcattt cttttttcca gttcatcagg taaaactcac gatccgcctg
     1081 caaaagttca cgcacttcca aggccgcata acacgtcctc tgtacaaagt ccgcatgagg
     1141 aatagggtca tttgaaaaag caagaatgcc atcaccttga aacttgtcga tggttaaatc
     1201 atacttcaag aaagtggaca ccaccgtgtc catgaagcgt gcaaggacca tatcaacttt
     1261 gcgctggtcc aatcgaacca cgcgctcagt cgatcctacg atatcgacaa aaatagcaca
     1321 aatttggctt ctatgaactc ctttgtccag atccacgcgt ccctctcgga tagctcttac
     1381 cacttgaggg ctaaactgac tactcagaac attgaggcgg acggcttcac cagttttctc
     1441 ttgaattata agatcgcgat tagaaatttc ggtcttcaaa gctaggcgag aattaatttc
     1501 tcgacttcgc aatgccccgt taaagtgacg gatcaaaaaa cataatacca gagagcccac
     1561 gataaaaaag gagaagacga aaatcgcctt ataatcttca atgccacgag cctttaatag
     1621 aacaatgata taatatggca gaaaaatgcc aatcgctaaa agagcgaaaa agcgacgact
     1681 aaaaggtaca aaagagagcc cccccaaggc aaccaaattt aaccctgcat agtaaggcgt
     1741 tgccgcatct ggcaccataa ggataatggc gttaataaaa ccagcagcac caacacccaa
     1801 tgccaagatc gccacaaatt gcgtttgatt aaaactccgt gtcctatcta taagaaagtt
     1861 aatccccaag cacaaaggga ttactaacag tcgaatccca aggaagggcc atttatactc
     1921 gggatataaa atacaatcag ccaaccaaaa aacaagaaac aatggaacca tcatccaatg
     1981 acaaatcacg tggacgatcg aacgcatttc ttcgatcgcc tctgcttcac gaaacttcgc
     2041 tgccgctaac ttttcttgca tactctcctg gagctgtccc aatttgcttc caacttgtcc
     2101 ttcgttaaat ttctattttt caatcactac aaaaagattt actcctaaag gttctttttc
     2161 gacataaacc ttagaaccga aacctttgaa catcctctcc agatcttctt ttgagcgata
     2221 aatcaaattc caatcaaggc acaactccat aagcggtgtt gtgggattgt ctgtgctgaa
     2281 attaccgata atgagctttc cacctggctt tatacatgca agcatttttt gagcagccat
     2341 ttgcgccacg ggttcagtga aataatcaaa taatcctgca gagtaaacca aggcatagtc
     2401 tttttctggc agaccaccgg caatcacatt gcgaatagcg agatttttaa aattgaactt
     2461 aaaacctgac ttcacaaacc tatctataga gccaatgtgt ttttgcgcgt acttcaaaga
     2521 ctcctcatct tgatccaagc aagtaaaaga cacgggacga ccataaaact ctttataatt
     2581 ctgaagaaag agttgttgtt ccatcgctgg accactggct acagatatga tctttagagg
     2641 ttcttgagat gaatgaactt caaaaatttg tttgattttt tcaactagat aatatccgcg
     2701 atttttaacc gccactcctg ccggctcacc cacaagatat ttatgcatgc attgatcaaa
     2761 tagagttttg cccgtcactt ccgcgcgata aagatgattc atcatctcat aatccccggc
     2821 gtaaccacgg ggtttatgaa aggcgcgtgc cgcaaaagga gctccataga tatagggtcc
     2881 gagttgttca cgaatgaaat ccgtggccag ttgagcatct tcaggactta aatttttcaa
     2941 catcggcggt acttgtttgt agacttgagg gattacattt ccaatatatt caccgatatt
     3001 ttccacaatc gtcatgcggt actcttgcgc ttcttgcgca ttatctacgg gagcatcttt
     3061 ttcgagttga tcaatagact ctttaagctt ttgaaaccag tcccggactt gaaaaacaat
     3121 ctgccgataa ggcggcggca aacgctctat tttagcccca tattcatttt gcttctgcaa
     3181 aaccgctgag gtcagttcga ggaccttgat acgctccacg cggaaaggct cccccaccgt
     3241 ttcaaaggcc catattctct cattcgtcac cgaagtggga tgagattcct ctctgacttt
     3301 acgtaaatgc aaagtctgtg tctccgtacc ctcaaagtaa accttggccg tgcagtattc
     3361 tttttcttga taaagtttcg cgagaatgag cgaatcctca gcactcacga tggttgcgca
     3421 accaaaggtg cttatgttca aaacttctat tcttgttcca tggaaatcca agaagcattt
     3481 tgccggatcc agctgtaagc gatcttgacg gataatcttc ttatactttg cggttccttc
     3541 ttcattgata agaaaacctt cttgtttcat agctgcagat gggcgagtca tgggtgattc
     3601 tccttgaatt tcatagttca tatatcggtt tatttaaaac gaacggtatg gctgatggtc
     3661 caggcagggg gtccagttcc tcgttaacac tatgcttaat gcggccaaga aatgggcaaa
     3721 ttgacgattg ccctggtcat tagaccctag agggcggtag actaaagtgt tacttgaagt
     3781 gaggaaaata tgcttgaagc agtcttaaca ggaattggct ccctcgtcgt tggaggcgga
     3841 ctcggattca ccctacgaaa ttatataaac acatacactc ttaaaaaagc taaacaagag
     3901 gccgcggact tgatcgccga ggccaatgat gcggtggaaa ttaaagctct cgaagaaaaa
     3961 gaacgcaccc aagagatcga actggagatg tggtccaaag ttgaacctga catgctcaaa
     4021 gtcgaagctc gcatagaaga actccaggag ctcattgacg aaaaaaagca aaaacacgat
     4081 gccgctctcg cagaagaaag aaaaaaactg caaattcacg agaatgaaac ccaacagcaa
     4141 gaaaaagctg tgcaagctca agaaatagct ttgcaaaaaa tccgtgcagc tcaaaagcag
     4201 ctgaatgatg aatttacgaa gcgcctttca cagaaactca acaccagccc ccaggaaacg
     4261 aagcaacaaa tcgctgaaac tctcgagcaa gagacttatc aacgtttgca aaagtgggcg
     4321 gaggaatacg aagaagagac taaagaacat gccgaaaccc gcgccaagcg aattcttgac
     4381 gtggctttag accgcttcgc ccgcccctac tgcgcggagc gcggcatagg ggctgtgaac
     4441 ttccccgatg ccaacgcacg ccgcttgttg tgtgatgcta agggtgaaaa catcaagacc
     4501 ctgcaggagg tcggtggctg cgatattatc gttgagccag aaatggagat ggtcggtgtg
     4561 gcaggttttg atcctgttcg tcgcgaactc acccgccgag tcatagagcg tgtactcaaa
     4621 gaaaagaaaa acctcactcc cgattttatc cgcaaagtag ctgaaaatca gaagaaggag
     4681 ctttttcgcc aaattaaagc cgatggcgat gccattgcca aagaattaag acagcaggat
     4741 cttcaccctg aaattaaaca aatgatggga agtttacgtt atcgttattc ctttacgcaa
     4801 aatcagtatt tccattgcgg cgaagtgggt tggcttgcgg gtcttctcgc agcagagtta
     4861 aaaatgaacg ttaaaacggc tcgtagatca ggcttgctcc atgatatcgg caaatccatg
     4921 gaccatgcga ttgatggagg ccatgccgtc ataggagcag acttcattca agctcgcaat
     4981 gagtcccctg aagtggttca tgcagtacga gcccatcact atgatgagca accttccacg
     5041 gaccatgcct ttctggttat tgccgccgat gcgatctctg gggctcgccc aggggctcgt
     5101 cgatcgacga ttgagtccta caatcaaaag gtttcagaat tgcaggatat cgccagaagc
     5161 ttcgatggcg tcaccgattg ttatgttctc aatggcggcc gtgaatgtcg agtgatggtg
     5221 aacagtcgca aagtggacga ctcgcaaact ctggctttag gtcgcaaaat cgcgacaaaa
     5281 attgaagagg aatgcaatta ccccggccaa attaaagtag ttattgtacg ggaaacggtc
     5341 gtctctgaaa gcaccaaggg tcacaactaa attcttcatc gaagacaaat aaaaaaggga
     5401 gccgaagctc ccttttttat ttgtctatat cttattcatc actacctacc cgtgggtggc
     5461 gtgatactgc tcttcactga agtcatccac taaaattgca tcgtagcgaa cggtatctga
     5521 ctgttgaata agtttgtact catcttcaga gatcacattc ttcccccgag catcatcaag
     5581 caacagggcc actttctttt taggcaacaa tccatcacgg atggccttct taattttctt
     5641 ctccgcagct tccgctctca aagtgatgct gaaagcattt tctagacgat gaagctgttc
     5701 cccttctttg gtcggcaaga aaataccttg ggtcaaacga tcccgtacag atccctcttg
     5761 caacatcgct gaagcaatgg cgtggctcca accgtcagat gcttgagaac ccagagagtt
     5821 gatacgcgac catgaaccga tccaaccttt aacaaaccaa cgaagacctg gaattttcaa
     5881 attatcgaaa agaccatcga aacccttttg gatttcagcc atacaaagtt tcaaattata
     5941 atgagcaaaa gccaaatcct cgtcgcgacg accctctgct tcaaaacgtc ttaagataga
     6001 tgttgccata tacatataac ctaagatgtc agcgaaacga ccggtaattt tctcacgcat
     6061 tttcaattcc ccccctaaga ctgccatggc aatatccgcc acgattgcat aagtcgcaga
     6121 agcccaggac agacgacgga aataacgttt catttgcgga tgcgcgctcg gagccggcgc
     6181 caaataacca cgagtcaggc tgagaccaat actacggcat agatttctaa ccacatggcc
     6241 cacgtgaccc cagaaagcgg tatcgaaagc gcccaggtct ttggcatcca tcgccttcac
     6301 ttccgcataa gcgaacggat gcgcacgcag agctccttga ccaaaaatga tcagggtccg
     6361 agtcataatg ttcgcgccct caaccgtgat accaatcgga gtgccgatat aaatttcagc
     6421 tagtaaattg cgaggtccca tggagattcc cgctccaccc ataatatcca tggcatcatt
     6481 gatgccttta cgacccatct ctgtagaata gtacttttga atcgctgtga tcactgaggg
     6541 tttcaaacct tgatcaagag ctcccaaagt atattttctc atggcttcca acaagtaagt
     6601 ggcagcaccc aggcgggcta gaggttcttc gacaccttca aatttaccaa tggaaacgcc
     6661 gaactgacgg cgaatgacag agtgagcgct cactacacgg gaagccaatt ttgctccacc
     6721 agtagcttgt gcgggcaagc taatgccacg gccggcagcc aagcattccg tcagcatcaa
     6781 ccaacctttg ccgcacccgt ctttgccccc taccacagcc tccaaaggca caaccacgtc
     6841 tttaccttga gttggacagt tatagaaagg agttcccaat ggatcatgac gacgaccaat
     6901 caccactcca ggagtgttag aagggatcag gccacaagta ataccaatgt cttcgcccat
     6961 tcccaaaagg ccttcaggat cgcgaagtct aaatgccaaa ccaatacagg ttgagatcgc
     7021 cgcaagggtg atccaacgtt tgttccagtt caagcgaata tacagttttc catccgtgcc
     7081 tttgaacacc acaccgctgg aaatgatcga acccgcatca gagcccgctg taggctcagt
     7141 caaaccgaag cagggaatct cttcgcctct tgccaagcga ggaagataat atttcttttg
     7201 ctcatccgtt ccgtaatgaa ccaacaactc tgcagggccc aaagaattgg gaaccatcac
     7261 ggtcactgcg gtggaaagag agcgcgaagc caatttcata ataacttcag aatgggccaa
     7321 agcggagaac cccagtccgc cgtattcttt tggaataatc attccgagga atttttcttt
     7381 tttaatataa gcgaaagcct catcggaaag tttgcgggat ttccacattt cccaatgatc
     7441 aatcttttta caaagttctt ccacgggccc attcatgaaa gcttgttctt cagcggtgag
     7501 ctgagggtaa ggctcctgca tgagtttaga aaactctggt tttccagaga ataaatcttt
     7561 ctccacccaa acagttccag catcaattgc cgcgcgttct gtatctgaaa tttttggcaa
     7621 gaagtggagc ttttcaaaga gagtcatcac ccctttggtt accagcgcgg cacgcaaagg
     7681 cttcacatta aagatgacca tcaccactgc ataaacaatc aaaagccaaa ccgggagttt
     7741 gaaacccaca aaaatcacag ccaatacgat tgtccaaata ataagaggac ttcctaaaaa
     7801 tccaaccgcc agaagaccaa caagagaacc aagaagggcc caccacgggc actctaaaaa
     7861 tagaccatga atgctattca cgctgcaact cctttaataa cgttaccaat tacacgcctg
     7921 tttttgagga agggcaaata aaaaagcctc cacattaagg tggaggcttt taaaactaat
     7981 gatcatcaaa tagagtctga gaattactta gtttcagagc caggagttgc tgctggagcc
     8041 tcacccgctg gagttgcagt cgctgcaggt tgaccttcag ctggagctgc catttgttct
     8101 tgagctgcag gcgtaggagc tggtgcagtt tcaactggag tgtttttaga gcaagtgaag
     8161 ccagtagtga aagacaatgc aagcaatgct gctacaacga ctaatttcat ggtgtaatcc
     8221 ccttattaat taataccttt cgcatttacg cagaacggca atggttaaaa aatcctaaat
     8281 ctgtactttg gtccacgtaa actgcacgaa acctcaaact tttgcaagaa tttatttctg
     8341 acaatttgaa caccaataac tcgagcggcc gcctaagatt tgagctgaaa tttccgtgct
     8401 acaaaccaca cacttttgtc ctttccgatc ataaactcga aaactttgct gaaaatatcc
     8461 gctttctccg ctgaactgtt cgtaattact aatgctggag ccaccggccg caatagcctc
     8521 tgttaaaacc tctttgatga tacgcgccaa tcgctcacat ttttcccggc tgactttcat
     8581 cgccttgatg gaggggcgga ttcccgctcg aaacagagct tcgctcgcat aaatattccc
     8641 cacaccgacc acaattcttt ggtccatgat ggccacttta atagccgtga ccttcccccg
     8701 cagcttcttc cacaaagcat cgccgctgaa ggcttccgtg aggggttcat agccaagaat
     8761 ttttagtttt gcatggttct cttcctgacc gagagccaca aaatcgaagc agccaaagcg
     8821 gcgtggatcc cgataagcca gacgcaagcc accccccaga tgcaaataga tatgatcatg
     8881 atcacgctcc tcccccagaa ctgccactcg ccacgttccc gtcatcccta aatgcgagag
     8941 caaacttccc gctggcgttt ttaaaagcaa gtacttggcc cgcctctcaa cggaaacgat
     9001 tctctgcccc accaagctct gcactttttt aactggaata ggatctcgca agtccttacg
     9061 cttcagctca atcttctcaa ttacggggtg atccttcaga atagctgcaa gcccattacg
     9121 tacggtttcg acctcgggaa gttctggcat taggcaccta acttgttgat attattagag
     9181 tgggatgcct gaaaaatact caaatcaccc aaaatcaccc gattccaaat cacccacgtc
     9241 attttaccct cctaaagggg actacattgt cacctaactt acgatcttct ttactcaacc
     9301 caagctgatt aatatgctct aaaactttaa gcgacgtttc tttttgaact tcgccgtcaa
     9361 ttttcgagta atgatcagca agctttaaat ctttatgacc tgtcatcgcc atcactgaat
     9421 ccaaacccat gccgcctacc cttcgagcca acgtcgccat cccatgcctt aaacaatgag
     9481 tgcccgtata aggaatccct gtttttgcct gggcccatct atagttcgac tgaatcgtac
     9541 aatagttcaa gggcttgcct tccacgtgaa ataagtaatt gcatccaggg gctctcattt
     9601 tcaatcttct ttcgacgatt tctttaagcg ccgaatggat atgcaccctt cgtgattctt
     9661 tgttcttagg aaaaggcttt agatactcaa acaccttact gctattacac caaaccacga
     9721 catccttaat cagaatgtat taataagtgc gttggataag ctacatattt cttgattttt
     9781 atcttggttt agttattctc agactatgag aaaagatgac gcaagaaaat tagatcacaa
     9841 aactctagaa gaattaagaa tcagatcagt tcgcagag
//
LOCUS       DATBTE010000116        19522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_697882, whole genome shotgun sequence.
ACCESSION   DATBTE010000116 DATBTE010000000
VERSION     DATBTE010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 19522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19522
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697882"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VIG33_04250"
     CDS             <1..755
                     /locus_tag="VIG33_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY8270074.1"
                     /translation="FLGKIKWPLLMVLVLAPLALLLKQPDFGSFAIILMVGIGLLFSF
                     GLRWRYILTVATMVLPAVYFLIMAVPYRRARVLAFLDPWSNPASTGFQVIQSMLSFHS
                     GGLTGAGIGQGQGKLFFLPEAHTDFTLAVFGEEMGFVGFLIILSLYGFVVMRGIQIAV
                     KVEDTFKKSMVLGLTLSFALSVFINAGVVMGLLPTKGLTLPFLSYGGSSLVVLCFTFG
                     LILNVENSLESGDLSKKFRKARWDQSKVKNHE"
     gene            745..1827
                     /gene="murG"
                     /locus_tag="VIG33_04255"
     CDS             745..1827
                     /gene="murG"
                     /locus_tag="VIG33_04255"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY8270075.1"
                     /translation="MMSKKNVVIAGGGTGGHIYPGVAIARAIEIKHPDYQIHFVGTPT
                     GLENKIIPREGFPLHHISIGKLNHAGDLKTKVLTILGMPRAFIQSIALLMELKPQAVL
                     GVGGYASGPFVLVASLLGFRTAIWEPNAFPGMTNRWLSRFVGKSFVVFEEAAKFLKSK
                     AITQVGLPIRKEVESLASKDIGKDAPEFHILVFGGSQGARSINKTVQEAILKGGEWLS
                     NTKIIHQTGPTDYASVAEAYKGHSQVEPHEYLYQMEKNYEWADLIICRAGASTVAEVS
                     ACKKPAIFIPLPTAADDHQRKNAETLLEQKAAVMILQKDLTPESLIQQIEYLKNDSQA
                     REEMRKNLAKFYKPQAAEKMAELLLG"
     gene            1847..3208
                     /gene="murC"
                     /locus_tag="VIG33_04260"
     CDS             1847..3208
                     /gene="murC"
                     /locus_tag="VIG33_04260"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY8270076.1"
                     /translation="MRLQKSKFHFVGIGGIGMCGLAELLHNMGAIVSGSDLAENANTE
                     RLKDIGVKIFKGHAATNVRDADVVVYSSAIQYTNPEIADARAKQIPLIPRAEALAEIM
                     RIKRGIAVAGTHGKTTTTSMTSAIFLEADLKPTIVVGGRFELIKSTALLGSGEWLVAE
                     ADESDGSFHKLSPEIAIITNIDSDHLDHFKTFDNLQKSFYDFALRVPFYGTTIICGDD
                     PKVNVLFENFPKRAHFYGFDEKNDIILKGENGKYTLFRRDYKTGEKNKFGDFELKVPG
                     RHNALNATAAILAGLAAGVDFSTCAKGLQRFEGVDRRFHFKGEKKGVRVYDDYGHHPT
                     EVRAVLQAFREKFPENRLIVFFQPHRFSRTQHCWHDFTTCFNQADEILVTDIYPAGEA
                     PIPGVNSEKLVEEMKHEKAEYFVRDPHSSDKILTRLHSGDIFITLGAGDGWKLGLEVL
                     NKL"
     gene            3239..4138
                     /gene="murB"
                     /locus_tag="VIG33_04265"
     CDS             3239..4138
                     /gene="murB"
                     /locus_tag="VIG33_04265"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY8270077.1"
                     /translation="MEIKKNVALAEYTSWLVGGSAEFFCLPENEADVREAMQWAHEQK
                     HAVTLFGGGTNVLISDKGIRGLVICLRKFSGVESKEVEGRLEITALAGTGKSELLKIF
                     LKNKLPPALFLAGLPGDVGGGVVMNAGVAEAYVPREFVEIIDWIEVLRFEVDTFTTHR
                     FTKDQLHWEYRHSKGWQPGVITRVGMSWPLQQADASILEQVRSANKIRLSKQPLDMPS
                     CGSVFQNPPGHKAAQLIDSCGLKGFTVGEAQVSLKHANFIVNLGEAKALDMWQVIEHV
                     RETVKKIKNVDLHTEVIRLGEWT"
     gene            complement(4135..4899)
                     /locus_tag="VIG33_04270"
     CDS             complement(4135..4899)
                     /locus_tag="VIG33_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEY8270078.1"
                     /translation="MYSFTNPKIVIIPLPSHDFDPTECAVPWKILTQNGVKVRFATPE
                     GQVARCDLRMLFGQGLGLLAPLLAADKNGKAAYEALEQSHEFRNPCKWSEIQAHVYDG
                     IILPGGHASGMKEYLESEILKKIIEDFFLLNKPIGAICHGVVLVARTLTSGKSVLTNR
                     KTTSLLARQELLAWGLTKIWLGDYYRTYPQTVEGEVRSVLNSQDGFLPGPMPILRDHP
                     LSLSRGFTVRDQNYLSARWPGDAHRFGRDFLEMVKN"
     gene            complement(4900..7401)
                     /locus_tag="VIG33_04275"
     CDS             complement(4900..7401)
                     /locus_tag="VIG33_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270079.1"
                     /translation="MKQWSLFLATCLIGLNAFATVPTVFQLTQRSLQIINIQDLFGSL
                     NRDEIRESLNDPKIESGEITFKYQMFLREEGKRLKLRSFSENFDKKIAEYEKTYSPQV
                     RQYIETVAQRHHAKIHKEGTTKIYSFQDGVGFFQDLYAFKDVKDQMIKSMVVALMDNP
                     LRQAKLASPLEKLIASVYKQTLAETFLLSADEFSEADLKIIATMEKQNSYRYEKVGNL
                     WILGSGFGFFITRDLVEKGNKLTDPKLKSRFEKIIRYHVNFQVPVTHEYQQGAAAFFN
                     PTDGKMTFVLPSRGDLNSYTHEMTHSRFNKFTKTLDAWTKAKGYRVPYQIDGPSLGGM
                     AALFANHGGLFNLLNEVNSWRTGEGFDGKGADGQILKTLVEGYGPQAGYEATELLEKV
                     WTPEKLAGKSVPYLIYSEIREFNKMTNDQLVMMGFEGVSEANLLKKMNFLIMYEAGRF
                     KGNSSEADANFVLGEITKNSKEKPVLDRLSKISSRNGSGDNVEPAKPDLSNATFEEVL
                     ENFRTTGSEEARDLLKEKFLKRIGPEHMEALVQRAVQAKTSLLSHEGVNVLEAVLNNV
                     KITLPVSEKNNSLSMRSPLKSSLLEALDTSLSVHLYDGTVASKKMDLLNFISKEVYPE
                     ELPKTYDKTLDIVKHTSERPNADVWLARLFFMPSGYNGGSEVLWAEKLIHDLKGTEVP
                     RNIKEAMGFFYKESLAKFISKNSYYGEYSNSNLNTKTRVLVLREIKKLSPRQESSLDT
                     ASHQLWSLAGDADPQVRVSALYAILSNPMYLVKIEGYMLQALYRKDSKYLSSVSFLLD
                     VGPEMAPQAKRIYDIQKENQSHGINSCEKVFMGSF"
     gene            7845..8066
                     /locus_tag="VIG33_04280"
     CDS             7845..8066
                     /locus_tag="VIG33_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8270080.1"
                     /translation="MNLLSKKVFDDYLSKSNALESDLELHQRKSDSNIVIIDEIQKLP
                     ELLDEVHNQIEKNKKLRFILTGSSARKLK"
     gene            8109..8669
                     /locus_tag="VIG33_04285"
     CDS             8109..8669
                     /locus_tag="VIG33_04285"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HEY8270081.1"
                     /translation="MFPLTFSEIGSQLKTISELERRLLIGGLPSIFDSKKPFDDFDDY
                     IQLYLNEEIKAEGLVRNHDAFHRFLQVSALTNAKEINISEIRAFLSYCGKKSELFYWR
                     STSKFEVDLIIKSSTQMWALEIKSKKVITKKERKGLLAFAEDYPKVKKIIVSLEGRHF
                     IDEDKIEIIPIFDFLKMLWADELGFD"
     gene            8798..9541
                     /locus_tag="VIG33_04290"
     CDS             8798..9541
                     /locus_tag="VIG33_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEY8270082.1"
                     /translation="MKKLILKLLVAFVMLPSVVAGSMYWLNEKGFFNVNQVEIILEDS
                     QSQVNFYSPMVEKLEKQMRAFKGQSLWALKLTQVTAILNSQNWIETHSLSRSWPSVLK
                     VKIKPHDIKLVYLGKGGKYLPIITDGSFLESVDSKQAPDVILLEGEVFAQKKELRQKA
                     VEVLKEIPAEGTFSKKTISEMHYDSKDGFSMVLMKTGIQVKIGDDLVALKSARVSQVV
                     DYLQTRQFDARVIDANLSKKVLVRLRNDP"
     gene            9663..10925
                     /gene="ftsA"
                     /locus_tag="VIG33_04295"
     CDS             9663..10925
                     /gene="ftsA"
                     /locus_tag="VIG33_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY8270083.1"
                     /translation="MSNIKPKAPVLAGLDIGSTKVCFVIGTVNPEGKIDVVGVGKAPN
                     TGIRQGVVVNIEATTESIRKAKEEAELMSGYAIADVWVGVSGTHITSFDSKGMVAIKN
                     KEVTTQDIERVIEAAKAVAVPADRNVLHVLPREYKVDGQDGISDPIGMSGVRLEASVH
                     IVTGGHTALQNSIKCVEKAGLHVSGLVLNQLAASAAVLSEDEKNLGVCVVDMGGGTCD
                     AVYFVNGSVAHTSIIPLGGYHFTHDVAVGLRTPQVSAEDLKRKYGCAMASMVNENETI
                     EVEGVGGRKSRLIPRKDLADVLEARAEENLNLIANDIRMSGLLPMLGSGLVLTGGASQ
                     LDGLVEMGEFIFDIPVRRGGPNKVGGLIDAVKSGEHSVAVGLLLYGLSQRKDLIVQSQ
                     QNQGEAINLGDSFDGMAQKVKDFFGQIF"
     gene            10992..12650
                     /gene="ftsZ"
                     /locus_tag="VIG33_04300"
     CDS             10992..12650
                     /gene="ftsZ"
                     /locus_tag="VIG33_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY8270084.1"
                     /translation="MFELEENISIGANIKVVGVGGGGSNAVSTMIESQMTGVEFIVAN
                     TDIQALNANKASSKIQLGLDLTKGLGAGANPDIGRRAAIESYNEIVEKLEGADMVFVT
                     AGMGGGTGTGGAPIVAKIARELGALTIGVVTKPFFFEGKKRTKHADSGLAELKENVDT
                     LIVIPNQKLLSIATERTPLLETFKKADEVLLQAVKGISDLINIRGLINLDFADIRTVM
                     ASKGIAIMGTGAAKGQNRAVEAATAAISSPLLENIRIDGATGIIINVTGGSDLSLLEV
                     NEASMLITEAAHEDAEIIFGAVIDDNLGEEVRVTVIATGFSQENRQMASDTINQMQQF
                     LNGTGIQFPSSGMNMLPPIPAIPNMMQQYQPQYQPQAPVMPAELPPIQTVQTSVMNYT
                     YQTVEQLPPQQQQQYQSSSSMTGTPQIPNQVPQNFQTQPGNSAQEPVATPIMPVPNMS
                     TAPQTPTPSQAPVMPEAPHPSEAAFSPRDLLMAKARAFKESQELKARHNPPEQLSMNV
                     DQEKQSLEEARKMAREVLSSPFSGQNLEVPAFIRKKQGFDLNKE"
     gene            12653..13402
                     /locus_tag="VIG33_04305"
     CDS             12653..13402
                     /locus_tag="VIG33_04305"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEY8270085.1"
                     /translation="MEAWFLSDIHLKTMQERNSEILLRFLRKLRTKNPQETHLFLLGD
                     IFDLWIGGHSVFVKKFRPLVEAIQELKEVGARITYIEGNHDVHIHSFWRDKLGVEVFV
                     EAQFYKINGLIVRVEHGDLINLEDAAYLRYRSLIRHPLIKPLGNMIPGKVWDAVGNYA
                     ARKSRVRSQSYRVTNEDKLVQMIRHHAHRAYNEKPYDLIVSGHMHIYDDYEFQVAARK
                     VRSINLGSWYGSEVKVLKLTGTEVEWVEIGK"
     gene            complement(13455..14177)
                     /locus_tag="VIG33_04310"
     CDS             complement(13455..14177)
                     /locus_tag="VIG33_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270086.1"
                     /translation="MSLLRKSMLSAVAGLLIMTFTAKNANAAGGGGHSLEFGFGLLTA
                     GQDDINTLIKTARVSATTANTSDMGSAYELFAQYQYRFSGTMFSIAFRPSYITQSATG
                     SGTDGSYNYKLSGFTVFPMFRLYPLENSFIKFYLQTGLGYGRMSGEVTEGAASISFAG
                     SAFGALGGLGVDFCFTPDHCLSVEGNLRYLPIERNVASSATGSAAGLSQATNGQEVEI
                     GGGDLRTSMSGIQGSIAYTLNF"
     gene            14283..14843
                     /locus_tag="VIG33_04315"
     CDS             14283..14843
                     /locus_tag="VIG33_04315"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY8270087.1"
                     /translation="MKKAEAKNLIEKKIKSELAYDGSFLKVLKDEVEFPNGKKGSREY
                     IPHPGAAMIIPITDQGRLIMIRQYRYAVQNIFMEFPAGKIDAGEDALQTAKRELEEEA
                     GLQAKEFRHLTTIHPVIGYSNEKIEIFLATSLTASQQHLDEEEFLEIFEISFEEALEK
                     MKKGEITDVKTMIGLFWYNQVLNSAW"
     gene            complement(14847..16934)
                     /locus_tag="VIG33_04320"
     CDS             complement(14847..16934)
                     /locus_tag="VIG33_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8270088.1"
                     /translation="MSPMKFYILFFICAVTFSSEAKTFKKNLSSAHVPNQLIVKFKNN
                     SSPMIQRSFLQRMNVQVVQQFKSNGAVLVQFPNTVIQKNSNALGLMAKTMNEASEIEY
                     VEPNWIIHANAMPNDPLYDKLYALNKSASRTGDIFAQEAWTKTTGSRDVLVAVIDSGV
                     DYTHQDLADNYWSNPKETGMDSSGRDKRTNGLDDDNNGFVDDWRGWNFVKNTNDPMDD
                     DYHGTHCAGTIGAVGNNGLGVVGVNWNVSIVGLKFLDNTGSGTLANAVLAIEYATSLG
                     VHVMNNSWGGDEYSETMVAAIQKANAAGILFVVAAGNDANNNDVGLDYPSSYPIDNVI
                     SVAAIDSEGQLAGFSNFGAKTVHIAAPGVQILSTVPGNNYEAHNGTSMAAPHVAGAAA
                     LIKARYPQLKASEIKARLMGGAVPTPGLQGQVMTGLLNINNSLDADLAPPSVPQNISV
                     LSTTASSLNLEWDTAVDNGRDKYAYSYEIRSSTSPLVSSEDWENAASVSLTILQQNSQ
                     RVQARADITLLNSSGYLAIRAVDRFGNRSELSNSVTYALERVTAVYANKGDTLDGLSL
                     EGPWGLEEVLGHGKVLSDSPGGTYGFAKTSTLTLPTLDVLTTDMVLSFNGKYDFELGY
                     DFGYLEISTDQGHTWRQIAMFNGARNWTPATYPLNTYLTPEDTTVTLRFRVTSDATTS
                     QDGWYISDISVFK"
     gene            complement(16997..18163)
                     /locus_tag="VIG33_04325"
     CDS             complement(16997..18163)
                     /locus_tag="VIG33_04325"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8270089.1"
                     /translation="MFQRLLKLPINNSFFIFGPRGSGKSTHLREHLLTPQSLYIDLLS
                     DQQEKRYRKNPDILISDVAARPDLDWVVIDEIQKIPKLLDLVHKLIEEKKVNFALTGS
                     SARKLKRDGANLLAGRAFILNMFPLTFLELKEKFMLSQVLRWGSLPRLFAYNNDEEKI
                     LFLNSYVKTYLNEEIISEQLVRNVEGFRDFLEIAAQMNGKIINFSKIGRESSMDNKTV
                     KEYFQILEDTLVGFWVPGFHQSVRKAQKFQPKFYFFDVGVQRALEGSLDSIPTPGTSS
                     YGQYFENYLFTEIFRANTYSQKNYKLSHYQTTTGQEIDLILSKGMKKIVVKIKSTTQI
                     DPVEVKAFARLAEAFKESQNYFVSLDPVATELYGVRCLYYQDFIREVFWPQVNL"
     gene            18339..19364
                     /locus_tag="VIG33_04330"
     CDS             18339..19364
                     /locus_tag="VIG33_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270090.1"
                     /translation="MREKNIIFNFNKMKRFTLTFGLITGLCFLGIPASAEPSGLIDVI
                     RSQEGVTSLTGPSCQELTQQERFLRLWTLNVGETPGPASVAVENADHSLCSLVINNSV
                     PSIVHLLQDFKTTFSGPNCWNTTLRLSRLIQLSRFSSAEEMSFWMTSPYCRELSKTEK
                     PLPGDIIAIRGEKPNQPGAFDEIHGMIYLSDDLVFSKNTSSSMSPYGIQRANLVYQIF
                     RTADPKCTKVQGRPEDCYRWVNHYRCTSSEKDQLDLEKENSDFSSLSGELKEIESQLS
                     EYVMNGQGNYDNNKEVIQKRLTDIEGKTLKLPKTPGKASFFAKALLQKINSLKVQTVI
                     LSKQPPR"
BASE COUNT         5423 a         4100 c         4614 g         5385 t
ORIGIN      
        1 acttcttggg gaaaataaag tggcctcttt tgatggtctt agttttagct cccttggcct
       61 tacttttaaa acaacctgat ttcggaagct ttgcgatcat cttgatggtg ggaataggcc
      121 tgctattttc ttttggtctt cgctggagat atattctgac tgtcgccact atggttttgc
      181 cggcggttta ctttctgatt atggctgtgc cctatcgtcg tgcccgagtc ttggctttct
      241 tagatccctg gtccaacccc gcttcgactg ggttccaagt cattcaaagt atgttgagtt
      301 tccattccgg ggggttaaca ggagccggaa ttgggcaggg acaaggcaag ctcttctttc
      361 ttccggaagc gcacacggat tttaccttgg cagttttcgg agaagaaatg ggctttgtcg
      421 gatttctgat tatcctgagt ctttatggtt ttgtggtgat gcgcggaatt caaatcgcgg
      481 tcaaagtaga ggacaccttt aaaaagtcga tggtgctggg attgactctt tctttcgccc
      541 tgtcggtgtt tattaatgcc ggggtggtca tgggactttt gccgaccaag ggtttgactt
      601 tgccgttctt aagttatgga ggtagttcct tggtggtgtt atgcttcacc tttggcctca
      661 ttttaaatgt tgaaaattct ttggaatcgg gggacctttc taaaaaattt agaaaagcgc
      721 gctgggacca atcaaaggta aaaaatcatg agtaaaaaaa atgtggtgat tgctggtggc
      781 ggcacgggtg gtcacattta tcctggtgtt gccattgctc gtgccattga aattaaacat
      841 cctgactatc aaattcattt tgtgggaacc cccacaggtc tagaaaataa gatcattccg
      901 cgcgagggat ttcctcttca ccatatcagt attggaaagc tcaatcatgc aggcgacctt
      961 aaaaccaaag tcctgacgat tttgggaatg ccccgagcct ttatccagtc cattgcctta
     1021 ttgatggagt tgaaacccca agcggttcta ggagtcggag gctatgcttc aggtcctttt
     1081 gtgctggtcg cgagcttgct gggctttcgt acggccattt gggagcctaa tgcatttcca
     1141 ggaatgacca atcgctggtt atcccgtttc gtcggaaaaa gctttgtggt tttcgaagaa
     1201 gcggcgaagt ttttaaaaag caaagcgatc acccaggtgg gtctgccgat tcgaaaagag
     1261 gttgaatccc tagccagtaa agatatcggg aaagacgcgc cagaatttca tattcttgtc
     1321 tttggcggaa gccagggagc tcgtagtatt aataagaccg ttcaggaagc gatcctaaag
     1381 gggggcgagt ggttgagtaa caccaagatc attcatcaaa cgggccctac agattatgct
     1441 tccgttgcag aagcgtacaa aggacatagc caagtggaac cccatgaata tctttatcag
     1501 atggaaaaga actacgagtg ggctgatttg attatctgcc gtgcaggggc aagcacagta
     1561 gcggaggttt cggcctgtaa gaaaccggct atctttatcc cccttcccac ggcagcggat
     1621 gatcaccagc gtaaaaatgc cgagactttg ttggagcaga aagcggcggt catgattctt
     1681 caaaaagatc taacccctga gtctcttatc cagcagatcg aatatttgaa aaatgattca
     1741 caagcccgtg aagaaatgag aaaaaattta gcgaagttct ataaacctca ggcggcagag
     1801 aaaatggccg agcttctttt gggctgaaat tagataggaa tatttcatgc gtttgcaaaa
     1861 atcaaaattc cattttgtag gtatcggcgg aatcggtatg tgcggtctgg cggagttgct
     1921 ccataatatg ggagcgatcg tttctggaag tgatctagcg gaaaatgcca ataccgaacg
     1981 tctgaaagac atcggcgtta aaatctttaa gggtcatgcg gccaccaatg tgcgcgatgc
     2041 ggatgtggtg gtgtattcca gtgcgattca atacaccaat cctgaaattg ccgatgccag
     2101 agccaagcaa attcctttaa ttccccgagc cgaggccctt gctgaaatca tgcggatcaa
     2161 gcgcggtatt gccgtggccg gaacccatgg taaaaccacg acgacgagca tgacctccgc
     2221 gattttcctg gaggcggatt tgaagccgac gattgtggtg ggcgggcgtt ttgaacttat
     2281 caagtccacc gctctcctcg gaagtggtga gtggctcgtg gcggaagccg acgaaagcga
     2341 cggcagtttt cataaattaa gtccagagat agccattatc accaatatcg actcggacca
     2401 cttggaccat tttaaaactt tcgataattt gcaaaaatca ttctatgact ttgctttgcg
     2461 cgttcctttt tacgggacca cgatcatttg tggtgatgat cccaaggtca atgttctttt
     2521 cgaaaatttc ccaaagcgtg cccacttcta cggctttgac gaaaaaaatg atatcattct
     2581 caaaggcgaa aacggaaaat acactttatt ccgtcgtgac tacaaaaccg gcgaaaaaaa
     2641 caagttcggg gattttgaac tcaaagtccc tggtcgtcac aatgccttga acgccacggc
     2701 cgcgatctta gcgggtcttg cggcgggtgt tgatttctcg acctgcgcaa aaggtctgca
     2761 acgctttgaa ggcgtggacc gtcgctttca cttcaagggc gagaaaaaag gcgttcgcgt
     2821 ttatgatgac tatggtcatc accccactga agttcgcgcc gtgctccaag ctttccgcga
     2881 aaaattccca gagaatcgcc tgattgtgtt cttccaacca catagatttt ccagaactca
     2941 acactgctgg cacgacttca ccacctgttt caaccaggcc gatgaaatcc tggtgacgga
     3001 tatttatccc gccggcgaag cccccatccc gggtgtcaac tctgaaaaac tcgtcgaaga
     3061 aatgaagcac gaaaaagccg aatatttcgt tcgagacccc cactcctcgg acaaaatcct
     3121 cacccgcctc cattcaggcg acatcttcat caccctcgga gccggcgacg gctggaaact
     3181 cggcctcgaa gtcctcaata aactttaggt acgccgtacc tttttcggat gcgatgcgat
     3241 ggaaataaaa aagaacgtgg ctttggcaga atatacttcg tggttggtcg gtggatcggc
     3301 agagttcttt tgccttccag aaaatgaagc ggatgtaaga gaggcgatgc agtgggctca
     3361 cgagcaaaag cacgctgtca ctctttttgg tggcggcacc aatgtgttga tttctgataa
     3421 aggaattcgc ggcttggtga tatgtttgcg caaattttca ggtgttgaaa gtaaagaggt
     3481 cgagggccgc ttggaaatca cggctttagc cggaaccgga aagtcggagc ttctcaaaat
     3541 atttcttaaa aacaaactcc cacccgcatt atttctggcg ggccttccgg gagatgttgg
     3601 cggaggcgtc gtcatgaacg cgggcgtggc cgaagcttat gtgccgaggg aattcgtcga
     3661 gattatagat tggatcgaag tgcttcgatt cgaagtcgac actttcacga cccatcgttt
     3721 tacaaaagac cagcttcatt gggaatatcg tcattcaaaa gggtggcagc ccggagtcat
     3781 cacccgtgtg ggaatgagct ggcctcttca acaggccgat gcctcgatcc tcgaacaagt
     3841 tcgcagcgcc aataaaatac gtctttcaaa acagcctttg gatatgccaa gttgtggctc
     3901 cgttttccaa aatccaccgg gccacaaagc ggcacagctc atagatagct gtggattgaa
     3961 gggctttact gtaggcgaag cccaggtctc gctgaaacac gcaaacttta tcgtgaatct
     4021 cggcgaagcc aaggccctcg atatgtggca ggtgatagaa cacgtgcgag agactgtcaa
     4081 aaaaatcaag aacgtcgatc ttcacaccga agtcattcgt ctcggagagt ggacttagtt
     4141 tttaaccatc tcaagaaaat cccttccaaa tcggtgggcg tctccgggcc aacgagcgga
     4201 gagatagttt tgatccctta cagtgaagcc tctagaaaga gaaagtggat gatcccttag
     4261 aattggcatg gggccaggta aaaaaccatc ttgagaattg aggacagagc gcacctcacc
     4321 ctcgacagtt tgtggataag ttctatagta atctccaagc caaatcttcg ttaagcccca
     4381 agcaagaagt tcttgtcgcg caagcaaaga cgtggtcttt cgatttgtta gcacgctctt
     4441 tccagatgtt agggttcgag ccaccaacac cactccatga catatagcgc ctattggttt
     4501 gttgagcaga aaaaagtctt ctataatttt tttgaggatt tcagattcaa gatattcttt
     4561 cattcccgag gcgtgtccac ccggaagaat aatgccatca tacacatgag cttggatttc
     4621 agaccatttg cagggattac gaaattcgtg gctttgctcg agcgcttcat aagcagcttt
     4681 accattttta tcagcagcaa gcagcggagc aagcagaccc agtccctgtc cgaaaagcat
     4741 acgtagatca caacgtgcta cctgaccttc aggggttgca aatctgactt tgactccatt
     4801 ttgtgtaagg attttccagg gcactgcgca ttcagtcgga tcaaagtcat gggaggggag
     4861 gggaattata actatttttg gatttgtgaa ggaatacatt tagaaggacc ccatgaatac
     4921 tttctcgcag gagttaatcc catgggattg attttctttt tgaatatcgt agatccgttt
     4981 tgcttgcgga gccatttcgg gtcccacgtc caacaagaaa gaaaccgatg aaagatattt
     5041 gctgtctttt ctgtaaagag cctgcagcat atatccttca atttttacta aatacatggg
     5101 gttcgataat atggcgtaaa gagccgatac tcttacttgc ggatcagcat ctcccgctaa
     5161 gctccacagt tgatggctgg ctgtatccag gcttgattct tgccgtgggc ttaatttttt
     5221 aatctctctg agtaccaaaa cccgcgtctt cgtatttaaa ttcgaattgc tgtattctcc
     5281 ataatatgaa ttttttgaga tgaacttcgc gagggattcc ttgtagaaaa atcccatagc
     5341 ttcttttatg ttccgaggga cctcggtgcc cttaagatca tgaatcagct tctcggccca
     5401 gagaacttca gagccaccat tatagccaga aggcataaag aagagccttg ctaaccagac
     5461 atcagcgttg ggtcgttcgc tggtgtgctt gactatatct agagttttat cataggtctt
     5521 cggtagttcc tcaggataga cttctttaga tatgaagttg aggagatcca tttttttgct
     5581 ggcgacagtg ccatcgtaaa ggtgcacgct caatgaggta tccagtgctt ccagcaaaga
     5641 agatttcagc gggctgcgca tagataaact gttgttcttt tcgctgacgg gtagagtgat
     5701 cttaacatta tttaaaacgg cttcgagaac gtttacacct tcgtggctca agagactggt
     5761 tttcgcttga acagctcttt gaacaagagc ctccatatgc tcaggtccga tacgcttaag
     5821 gaatttttct ttgaggagat cacgagcttc ctcggagcct gttgttctga agttttccag
     5881 aacttcttcg aatgtcgcat ttgaaagatc cggttttgca ggttcaacgt tgtcacctga
     5941 tccattgcgg gaagaaattt tggaaagacg atctagaacg ggcttttctt tggagttctt
     6001 ggtgatttct ccaagaacga agtttgcatc agcttccgaa gaatttcctt taaagcgccc
     6061 tgcttcatac ataataagga aattcatttt cttaagaaga tttgcttcag agactccttc
     6121 gaagcccatc atgaccaatt ggtcgttggt cattttgttg aattcacgga tttcactata
     6181 gatgagatag ggaacggact ttccggccaa tttctcgggc gtccatactt tttccagcag
     6241 ttctgtggct tcatagccgg cttgaggtcc atagccctcg acaagagttt ttagaatttg
     6301 cccatcggca cctttaccgt caaaaccttc accggttctc caagagttga cttcatttaa
     6361 cagattaaaa agacctccat ggttggcaaa gagtgcggcc atgccgccta agctcggtcc
     6421 gtcgatttga taaggaacgc ggtagccctt tgcttttgtc caagcatcca aagtttttgt
     6481 aaatttattg aaacggctgt gagtcatttc atgagtatag ctgtttaaat ccccgcgact
     6541 gggaagaacg aaagtcattt ttccatccgt cggattgaaa aaggcggctg cgccttgttg
     6601 gtattcgtgg gtgactggaa cttgaaaatt cacatgatag cgaatgattt tttcgaatct
     6661 gctttttaat tttggatcag tcagtttatt acctttttcc acgagatccc gggtaataaa
     6721 aaatccaaaa ccgcttccca atatccaaag gttgccgact ttttcataac gataggagtt
     6781 ttgtttctcc atcgtagcga ttattttcaa gtcagcttca ctaaactcgt cagctgagag
     6841 aagaaatgtt tcagctagtg tttgtttata aacgctagcg atcagcttct cgagtggact
     6901 ggcaagcttc gcttgtctta gagggttgtc catgagggcc acgaccatcg atttgatcat
     6961 ttgatctttg acgtctttaa aagcataaag atcctggaaa aaccccacac catcttgaaa
     7021 ggaatatatc tttgtcgtgc cttctttgtg gattttggca tgatgtcttt gcgcgacggt
     7081 ctctatgtac tggcgaactt ggggggagta tgtcttttca tattcagcta tttttttgtc
     7141 gaagttttca gaaaaagatc tgagttttaa tcttttgcct tcttcgcgca ggaacatttg
     7201 atatttaaag gtaatttcac cgctttcaat ttttggatca ttgaggcttt ctctgatttc
     7261 atcgcgattc agacttccaa agagatcctg gatatttatt atttgcaggc ttcgttgagt
     7321 cagttgaaaa accgtcggca ccgttgcaaa agcattcagt ccaatcaaac aagttgctaa
     7381 aaaaagactc cattgtttca ttcaaaggct ccttaaaaag ttggcgagtg actggcataa
     7441 gaagcaaaat tgactccaac aagaactccg tcgtggaggg caggattttt gaaaaaactt
     7501 tgatcgaatt cgtggaagtt attgggtttt aatcatttta tctagagggc taggcagcgc
     7561 caaggatggt ataagacatg gacaggtgac agttattgta atttttcaga ttaaaaatat
     7621 ttcaccaatt ttaatcattt ttggtgatac agatgccaat tttcatcatt tttggtggta
     7681 gaatagcata tgcacatata taatgtctta aaagtaagga gttgtatgta tcgcagaata
     7741 atagacatta gcactgtcct tagccgcaaa agtgtttttc tttttggtcc gcgacagaca
     7801 ggaaagtcca cttggttgcg taaaaattat ccgaagtctc ttacataaat ttgctctcaa
     7861 aaaaagtctt tgatgactat ttatccaagt cgaatgcttt agagtccgat ctggagctgc
     7921 atcaaagaaa aagtgattca aatatcgtga ttatcgatga gattcaaaaa cttccagaac
     7981 tcttagatga agttcataat caaatcgaaa aaaataaaaa gttacgcttt atcttaacag
     8041 gcagcagtgc gcgtaagctt aagtgatccg ggccaatctt ttaggggggg cgtgcttctt
     8101 ggcgtaatat gtttcctctt actttttctg aaataggttc gcaactaaaa acgatctcgg
     8161 aattagaacg tcgcctctta atcggggggc tgcctagtat ttttgattca aaaaagccat
     8221 tcgatgattt tgatgactat attcaattgt atctgaatga agaaattaaa gctgaagggt
     8281 tggttagaaa ccatgatgct ttccatagat ttttacaagt atcggctttg actaatgcca
     8341 aagagatcaa tatttcagag attcgagcct ttctgtctta ttgcggcaaa aaaagcgaat
     8401 tattttattg gcgatcaact tcgaaatttg aagtcgattt gattataaaa tcttcgacgc
     8461 aaatgtgggc cctggaaatc aaaagcaaaa aggttatcac caagaaggaa cgtaaaggac
     8521 tcctggcttt tgccgaagac tatccaaagg tgaaaaaaat tattgtgagt ctggaaggac
     8581 gacattttat tgatgaagat aaaattgaaa ttattccaat ctttgatttt ttaaaaatgt
     8641 tatgggccga tgagttagga tttgattaag attctcgtat cccctagctc tctgaaacac
     8701 gcccaaagtc cagcccgcgt aaatgcgttc aacacctcgt gggccttttg ttataatgtc
     8761 ctcaatacta tcgtggcatt agcgaacggg gttgcatttg aaaaagctca ttttaaaatt
     8821 actcgtggcg tttgtgatgt tgccgtccgt ggtggcgggc tcgatgtatt ggctgaatga
     8881 gaaaggcttc tttaatgtga atcaagtgga aatcattttg gaggattcgc aatcgcaagt
     8941 gaatttttat tctccgatgg tggaaaagtt agaaaagcaa atgcgtgcat ttaaaggcca
     9001 atctttgtgg gccttaaagc tcactcaagt gaccgcgatt ttgaattcac aaaattggat
     9061 tgaaactcat agcttgagtc gaagttggcc cagtgttttg aaagtgaaaa tcaagccaca
     9121 tgatattaag ctcgtctatt taggcaaagg tggaaaatat ttaccgatca ttaccgacgg
     9181 aagtttttta gagagcgtgg attcgaagca agctcccgat gtcattcttt tagaaggaga
     9241 agtgttcgcg cagaaaaaag agctgcgcca aaaagcggtg gaagttttaa aagaaattcc
     9301 cgctgaagga accttcagta agaaaacaat ttctgaaatg cattatgatt ccaaagatgg
     9361 tttttcaatg gtgctgatga agactggcat ccaagtgaaa atcggtgatg atttggtcgc
     9421 actaaaaagc gcgcgcgttt ctcaagtggt ggactatctt caaacacgtc agtttgacgc
     9481 tcgcgtcata gacgcgaatc tgtcaaagaa agtccttgtc aggttgcgca acgatcccta
     9541 agctaaagtc aaggtatact gaagtctagt gacttacgtc tatctcactc cctgaagaat
     9601 tagggagatg aaaatagttt tagataagat gtttggtcgg attttattgt taggacttaa
     9661 ggatgagcaa cattaaaccg aaagcaccgg tgctggctgg ccttgatatt gggtctacga
     9721 aagtttgctt cgtaataggt acggtgaatc ctgaaggtaa gattgacgtc gtaggagtgg
     9781 gaaaagctcc gaacacaggc attcgtcaag gcgttgttgt taatatcgaa gccactaccg
     9841 aatccattag aaaagcgaaa gaagaagccg aactcatgag tggctatgcc attgccgatg
     9901 tttgggttgg agtttccgga actcacatta cttcttttga ttcaaaaggc atggttgcca
     9961 tcaaaaataa agaagtgacc actcaagata ttgaacgcgt gattgaagcc gcgaaggcgg
    10021 tcgcagtgcc ggcggatcgc aacgttttgc acgtccttcc gcgtgagtac aaagtggatg
    10081 gtcaagatgg gatcagcgat cccattggta tgtcaggggt tcgtcttgaa gcttccgtgc
    10141 atatcgtgac tggtggtcac acagctttgc aaaattcaat taagtgtgtg gaaaaagcag
    10201 gccttcatgt gagcggtctt gtgctcaatc aattggcggc ttcggcggcg gttctcagcg
    10261 aggacgaaaa aaatcttgga gtctgcgtcg ttgatatggg tggtggtact tgtgatgctg
    10321 tctattttgt aaacggcagc gtggcccaca cttcgattat tccgttaggc ggttaccact
    10381 tcactcatga tgtggcggtg ggcttaagaa ctcctcaagt ctctgcggaa gatttaaaga
    10441 gaaaatacgg ttgtgccatg gcttcgatgg tgaatgaaaa tgaaaccatt gaggtagaag
    10501 gggtgggcgg tcgtaaatct cgcttaattc ctcgcaaaga tttggctgat gttttggaag
    10561 ctcgtgcgga agagaattta aacttgattg ccaacgatat acgtatgagc ggattattgc
    10621 ccatgttggg atcaggcttg gtattaacag ggggcgcaag ccagttggac ggcctcgtgg
    10681 aaatgggaga attcattttt gatattcccg ttcgtcgtgg cggtccgaat aaagtgggcg
    10741 gacttattga tgcggtgaag tccggtgaac attcagtcgc agtgggttta ctattgtacg
    10801 gattgagtca acggaaagac ttgattgtac aaagtcagca aaaccaaggc gaagcgatta
    10861 acttgggcga ttccttcgat ggaatggctc agaaagttaa agattttttt ggacagattt
    10921 tttaaatttt atctcatggg gttaatagtt atcggcgcag ggatgcgcac aaaaagtttt
    10981 aggaggaacg aatgtttgag ttagaagaga acatcagcat cggtgcaaac atcaaagtgg
    11041 tcggggttgg cggcggagga agcaatgccg tatcaaccat gatcgagtca caaatgaccg
    11101 gagtcgagtt cattgtggcc aatacggata tccaagcttt gaatgccaat aaagcatctt
    11161 ctaaaattca attaggtctg gatttgacta aaggtttggg agcaggagca aaccccgata
    11221 tcggccgtcg cgcagcgatt gagtcttaca atgaaatcgt tgaaaaactt gaaggcgcgg
    11281 acatggtttt cgttaccgcc ggcatgggtg gcggaaccgg tacaggggga gctccgatcg
    11341 tagcaaagat tgcccgtgaa ttgggcgctt tgaccattgg ggttgtcacc aagcctttct
    11401 tcttcgaagg taaaaagcga actaagcacg cggacagcgg tttggcggag cttaaagaaa
    11461 acgtagacac actcatcgtc atcccaaatc aaaaattact ttccatagcg acagaaagaa
    11521 ctccactttt agaaaccttc aaaaaagcgg atgaagttct tttgcaagca gtgaaaggga
    11581 tttcagattt aatcaatatt cgtggtttga tcaatttgga ctttgcggat attcgcacgg
    11641 tgatggcttc taaagggatt gccattatgg gtacgggtgc ggccaaaggt caaaaccgtg
    11701 cagtggaagc agccactgcg gcgatctctt ctccgcttct ggaaaatatc cgtatcgatg
    11761 gtgctactgg catcatcatc aacgtgactg ggggatcgga tctgagcctg ctggaagtga
    11821 atgaagcttc tatgcttatc actgaagcgg cccatgaaga tgcggaaatc attttcggtg
    11881 cggttatcga tgacaacttg ggtgaagaag ttcgcgtgac tgtgatcgcg accggtttct
    11941 ctcaagaaaa tcgtcaaatg gcgagcgata ctatcaacca aatgcagcag tttttaaatg
    12001 gtacgggaat tcaattcccc agcagtggaa tgaatatgct tccgccaatc ccggcaattc
    12061 cgaacatgat gcaacagtat cagccgcaat atcaaccaca ggcaccggtg atgcctgctg
    12121 aattgccgcc tattcaaaca gtacagactt cagtcatgaa ttacacctat caaacggtgg
    12181 aacagcttcc acctcaacag cagcaacagt atcaatcctc ttcttcgatg actggcactc
    12241 cgcaaattcc taatcaggtg ccacaaaatt ttcaaactca gccgggaaat tccgctcaag
    12301 agccagttgc tacaccgata atgccggtgc caaatatgtc gacagcccct caaactccaa
    12361 ctccttcgca agctcccgtg atgccggagg cgcctcatcc ctccgaagcc gctttttctc
    12421 cgcgagattt gttgatggct aaggctcggg cttttaaaga aagtcaggaa ttaaaagctc
    12481 gtcataatcc accagagcaa ttatccatga atgtggatca ggaaaagcag tctttggagg
    12541 aagctcgaaa aatggcgaga gaagttttga gctctccgtt ctcgggacaa aatcttgaag
    12601 tgccggcttt tattcgtaag aaacaaggct ttgatctcaa taaagaataa aattggaagc
    12661 ctggttttta tctgatattc atttaaaaac aatgcaagag cgcaacagtg aaatactgtt
    12721 gcgctttttg cgtaagttgc gaacgaaaaa tccacaagaa actcacttat tccttctggg
    12781 tgatattttt gatctttgga tcggcgggca ttcagtcttc gtcaagaaat tccggcccct
    12841 ggtggaggcc attcaagagt taaaagaagt cggcgctcgg atcacctata ttgaaggaaa
    12901 tcacgacgtt catattcact ctttctggag agataaactg ggtgtcgagg ttttcgtgga
    12961 agcccagttc tataaaatca atggtttaat agtgagagtg gaacatggtg atctgataaa
    13021 cctagaagat gccgcctacc tccgttatcg atccctcatc cgtcatcctt tgataaagcc
    13081 tctgggaaat atgattcctg gaaaggtctg ggatgcagtg ggaaactatg ctgctcgcaa
    13141 aagtcgagtg cgcagccaaa gctatcgagt tacgaatgag gacaagttag tgcagatgat
    13201 tcgtcatcat gctcatcggg cctataatga gaaaccctac gatctgattg tttcaggaca
    13261 tatgcacatt tatgacgatt atgaatttca agtagctgct cgcaaagtcc gttctattaa
    13321 tttaggaagt tggtacggat cggaagtgaa ggtcttaaaa ttgacaggaa cggaagtgga
    13381 atgggtggag atcggaaaat aaaaaaagcc agcatgggca ggatgctggc ttaagtgtaa
    13441 agatttaaat caaattagaa attcaaggtg taagcgatgc tgccttgaat tccagacatg
    13501 gaggtcctca aatcgccgcc gccaatttcc acttcttggc cattggttgc ttggctaagg
    13561 cccgccgcgc tgccagtggc agagcttgcc acgtttcttt cgataggaag ataacgaagg
    13621 ttcccttcca cgctcagaca gtgatcaggc gtaaaacaga aatcaacgcc gagaccaccg
    13681 agggcgccaa aagcgctgcc ggcaaaactg atagaggctg cgccttctgt aacctcgccg
    13741 ctcatgcgtc cgtaacccaa accggtttgg aggtagaatt taataaagct gttctccaga
    13801 gggtagagtc tgaacatcgg aaacacagtg aatcctgaga gtttataatt ataagaccca
    13861 tccgtgcccg agcctgtagc gctttgagtg atataagaag gacgaaaggc gatcgagaac
    13921 atcgtgccgc taaaacggta ttggtattgg gcaaaaagtt cataagcaga acccatgtcg
    13981 ctggtgttcg ccgtcgttgc ggacacccgc gcggtcttga tcaaagtatt tatgtcatct
    14041 tgaccagcgg ttaaaagacc aaagccaaat tctaaagagt ggccgccacc accggcagcg
    14101 ttcgcatttt ttgctgtgaa agtcataatc aaaagaccgg cgacagcaga aagcatagat
    14161 tttcttaata aagacatttc ctatcctcct ggatattttt acatctataa tagagtttaa
    14221 gaggagactc agacggagca agtccttgtc cctcttttgg acaaaggacc agaaagaacc
    14281 gaatgaaaaa agctgaagca aaaaatctca ttgaaaaaaa aataaagtca gagctggctt
    14341 atgatgggtc ttttcttaaa gttttaaaag atgaggtcga gttccccaat ggcaaaaaag
    14401 gcagccgaga atatattcct catccgggag cggcgatgat catacccatt acggatcaag
    14461 gtcggttaat catgatccga caatatcgct acgctgtgca aaatatattc atggagtttc
    14521 cagccggaaa aatagatgcc ggagaagatg cattgcagac tgcaaagcgg gagctcgaag
    14581 aagaggcggg tttgcaagcc aaagaattca ggcatctcac aacgattcat ccggtgattg
    14641 gctattcgaa tgagaaaatc gagatctttt tagcaacctc tctaacggcg tctcaacaac
    14701 atctggatga agaggaattt ctcgaaattt tcgagatttc ttttgaagag gccctggaaa
    14761 agatgaaaaa aggcgaaatc acggatgtga aaacaatgat tggacttttt tggtacaatc
    14821 aagttttgaa ttccgcctgg tgagaattat ttaaaaacag aaatgtcgga aatataccat
    14881 ccatcttgcg aggtcgttgc atcgctggtc acccggaaac gcaaagtcac agtggtatcc
    14941 tctggagtta aataagtatt cagaggataa gtggcggggg tccagtttcg agccccattg
    15001 aacatggcga tttgtctcca agtgtgccct tgatccgtgg agatttccaa gtatccaaaa
    15061 tcgtaaccca gctcaaaatc atatttgccg ttaaaggata aaaccatgtc cgttgtgagc
    15121 acatccaagg tgggtaaggt tagagtgctg gtttttgcaa aaccataagt tccgcctggg
    15181 ctgtcggaaa ggacttttcc atgccctagg acctcttcaa gcccccaagg tccttcaagg
    15241 gagagtccgt ccaatgtatc tcctttattc gcgtaaaccg cagtcactct ttccagggcg
    15301 taagtcacac tgtttgaaag ctcactccta tttccaaagc gatccaccgc acgaatggct
    15361 aaatagcctg aggaattaag aagcgtgata tcagcgcgag cctgcaccct ttggctattc
    15421 tgttgtagaa tcgtcagtga aacagaagcc gcgttctccc aatcttctga agaaaccagg
    15481 gggctcgttg aacttcgaat ttcatagctg taggcatatt tatcccttcc attatccacg
    15541 gcggtatccc attctaaatt taatgaggaa gccgtcgttg aaaggactga aatattttgc
    15601 ggaacactgg gcggcgccag atccgcgtct aaagaattgt ttatattcaa tagcccagtc
    15661 atcacttgtc cctgcagtcc aggagtcgga acggctccgc ccatcaggcg ggcttttatt
    15721 tcactggctt ttaattgggg atatcgggct ttaatcaaag cggcggcacc ggcaacatga
    15781 ggggccgcca tggaagtccc attgtgggct tcatagttat ttcctggaac tgtggaaagg
    15841 atttgcaccc caggcgcagc aatatgaact gttttagcgc caaaattcga aaaccctgca
    15901 agttgtcctt cactgtcaat tgcggccacg gagatcacat tatcaatcgg ataagaagac
    15961 gggtaatcta aacccacatc gttattattg gcatcgtttc cggcggcaac gacaaataaa
    16021 attcccgctg cattcgcttt ttgaatcgcg gccaccatgg tttcggagta ttcatcgccg
    16081 ccccaactgt tgttcattac atgaacgccc agggaggtgg cgtattctat cgctaaaacc
    16141 gcattggcaa gagttcctga tcccgtgttg tccaagaact ttaaaccgac aatggaaaca
    16201 ttccaattaa cacccacgac accaaggcca ttattgccga cggcaccgat agtccccgca
    16261 caatgagtcc cgtgataatc gtcatccatg ggatcattag tgtttttaac gaagttccag
    16321 cctctccagt cgtcaacaaa tccgttgttg tcgtcgtcga ggccattggt tcttttgtct
    16381 cttccgctgg aatccattcc agtttctttt ggattactcc aataattgtc cgccaaatct
    16441 tgatgggtgt agtccacgcc ggaatcaatc acggccacca gaacatcgcg agatcccgtg
    16501 gtctttgtcc aggcttcttg agcgaagata tcacctgtgc gggaggcgct cttattgagc
    16561 gcatataact tgtcataaag cggatcattg ggcatggcgt tcgcatgaat aatccaatta
    16621 ggttccacat attcaatctc tgaggcctca ttcattgtct tagccatcaa gcctaaggca
    16681 ttggaatttt tttgaatgac agtgttggga aactgaacga gaacggcgcc attggattta
    16741 aattgctgaa cgacttgcac attcattctt tgcaaaaaag atctttggat catgggagaa
    16801 ctattgtttt taaatttcac aatcaactga ttaggcacat gcgctgagga gagatttttt
    16861 ttgaaagtct tggcttcact ggaaaaggtg acggcgcaga taaaaaacaa gatataaaat
    16921 ttcattggcg acatgatggt cctctcgatg aaatcagact atagtgcatg caaaagcctt
    16981 gtccagacga gcctcgtcaa agattcactt ggggccagaa gacttcgcgg atgaaatctt
    17041 gataataaag gcaccgaacc ccgtaaagtt ccgtcgctac aggatccaaa gatacaaaat
    17101 aattctggct ttctttgaaa gcttccgcca aacgcgcgaa cgctttcact tcaacggggt
    17161 caatctgggt tgtggattta atcttgacaa caattttttt cattcctttg gaaaggataa
    17221 gatctatttc ttggcccgtg gtggtttggt aatgactcaa tttataattt ttctgtgaat
    17281 aggtattggc acggaaaatt tctgtgaaga gataattttc aaaatattgc ccgtaagatg
    17341 aagttccagg cgtaggaatg gaatctaagg agccttcgag ggctctttgt actccgacat
    17401 caaagaagta gaattttggc tggaattttt gcgccttccg cacggattga tgaaaccccg
    17461 ggacccagaa acctacgaga gtatcttcca ggatttgaaa atattctttc acggttttgt
    17521 tatccatgga agattcccgg ccaattttag agaaatttat gattttccca ttcatttggg
    17581 cggcaatttc taaaaaatcc cgaaagcctt ccacattccg aacgagttgc tcagagatga
    17641 tttcttcatt taagtatgtt tttacatagc tatttaaaaa aagtattttt tcttcatcat
    17701 tattgtaagc aaagagacga ggtaaagaac cccaacgaag aacctggctt aacataaact
    17761 tttcctttag ttcgagaaaa gtgagaggaa acatattaag aataaaagcc cgccccgcta
    17821 ataaattagc cccgtctctt tttagttttc gggcactgga gcctgtaagt gcgaagttga
    17881 cttttttttc ttcgataagc ttgtggacaa gatcaagaag tttaggaatt ttctgaattt
    17941 cgtcaatgac cacccaatct aaatcggggc gagcagccac atcggaaatt aaaatatctg
    18001 gattctttct atagcgcttt tcctgttgat ctgagagaag gtcaatataa agactttggg
    18061 gcgtcaaaag atgttctcga agatgggtcg attttccact tccacgcggg ccaaatatga
    18121 aaaagctgtt attaatcggt aacttaagaa gcctttggaa catattccta gattctaaac
    18181 gaaataagga gttaattcaa cgaaaatcga taggtattag atttttacag tctcgtctag
    18241 agaacgaaca gtcattcttc ctttagatta aattaaaggc gattacagga tggcatgttc
    18301 gtcgcaaagc tttgattact aagtagaggg cataagctgt gagagaaaaa aacataatat
    18361 ttaactttaa taaaatgaag cgattcactt taacttttgg tctgatcaca ggcttatgtt
    18421 ttttaggaat tccggcctct gctgaacctt ccggattaat cgatgtcatc cggagccaag
    18481 agggtgtgac ttctctcacg ggaccttcct gccaagagtt aactcaacag gagcggttct
    18541 tgcggctctg gaccctgaac gtcggagaaa ctccggggcc ggcatccgtc gccgttgaaa
    18601 atgcagatca cagtctgtgc tccttagtta taaataattc tgttccaagt attgtgcatt
    18661 tgctgcagga ttttaaaacc accttcagcg gaccgaattg ttggaataca actcttcgct
    18721 tgagccggtt gattcagctc tcgcgatttt caagtgcgga ggagatgagt ttttggatga
    18781 cctcccctta ctgcagggag ttgtctaaga ccgaaaaacc attgcctgga gacatcattg
    18841 cgatccgggg ggaaaaaccg aatcagccag gcgctttcga tgaaattcac ggcatgattt
    18901 acttatccga tgatttggtc ttttcaaaaa atacttccag cagtatgagt ccctatggaa
    18961 ttcaaagagc taatttagtc tatcaaatct ttcgcacagc ggatccgaag tgcacgaagg
    19021 tgcagggtcg acctgaggat tgttatagat gggtcaatca ttaccgatgc accagctcgg
    19081 agaaggatca attggatttg gaaaaagaaa attctgattt ttcgagtctc tcaggagagc
    19141 taaaagaaat tgaatcacaa ttgagtgagt atgtgatgaa tggccagggt aattacgaca
    19201 ataacaaaga agttattcaa aagcgcctta cggatattga agggaaaact ctgaagctcc
    19261 cgaagactcc gggtaaagca agcttctttg caaaagcact tctgcaaaaa attaattctt
    19321 tgaaagttca aactgtgatt cttagcaagc agccccctcg ttagacattt atgcatgact
    19381 aaatgaattc attgaccata ctcaataaat gccggacaat tattgggtat ggtcaataga
    19441 tttcttaagt attcaaaaac aaacagtttt tttcttttcg ggaatagggg ggtcggtaaa
    19501 tccacctatt tggaaggaca at
//
LOCUS       DATBTE010000117        15016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1586105, whole genome shotgun sequence.
ACCESSION   DATBTE010000117 DATBTE010000000
VERSION     DATBTE010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 15016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15016
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586105"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VIG33_04335"
     CDS             complement(<1..246)
                     /locus_tag="VIG33_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270091.1"
                     /translation="MKKLVTTVLLAIIAASNAALASDTFVPMYCGRNTRFKIFEPVKI
                     ETVCVGYDSDVREHSNWVRIQYTLSNGDTRIDIPRKND"
     gene            complement(741..3557)
                     /locus_tag="VIG33_04340"
     CDS             complement(741..3557)
                     /locus_tag="VIG33_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY8270092.1"
                     /translation="MKLKSFLRTFLFSFSFAAQLIWAAGVVHRSLNISALSPAIQKEL
                     VLRFPQVTGNKITLETIDEIIRFLQLRPEFDLVQAWDLGNGNLTLNTERATRVSAIKF
                     EGLRAISESEAKQLFGINLNEVLNQDQLINGGKKLRQQYNQIGFRNTSIDVEIPPDGK
                     GDVQLLVKVKEGVQTLIGAINLNSPHTDLNKALENRLSRERGYALTDVQLAEILKKIR
                     DYLNAKFYLRTEIKEPEITFNANESKAQLTLTLEKVDSYIIEYTGNREISSSALNNSL
                     DIGNFYSANPSIGAELSTKIKNLYLSRGYARVEVRSEDLEGRKPFTRKIVFNIDEGPR
                     VKIAKFEVSGNISRPSDFYVNLLKKQSSELIQKGYYNKAELDQSFEAMRIELQNQGFL
                     LARINSTRTQYNKEKNQVSVHVNLDEGPLTRIESIQFTGNETVPAAELLKAIDLASQQ
                     TLRLAQLEKAIQSLKIYYQERGYIDMTLLNEKEDLVSYNDNNTLAKLNFKIYEGPQVR
                     IANIILDGNTFTKDYVLLKEIDLQTGDTVTPGKLDEAIAKLQRTGYFNTVDVKTLEEK
                     TSVANRTLVVKVSERDPGLFTLGAGANNDLGLTLRGYTGLAYNNLMGTGRGISLRLEG
                     NYNVNEIKYLESKVTLGYVEPYLFDSKIRGRVNLTRSSLLSDYTTKKVSDVRQTTWSL
                     ERDLTSHITVIYELWNSAHLQDYFLHPAPGTPTPAPQDIVTTGPRLDIDYRDNPFNPT
                     KGHFTTLSYEYSSPDIGSSREGEIKFQRVTGSFTHYMRLPYLKDNFMIWANAIRGGYL
                     ENLSQKSGATDGVPYDKKGFYLGGISTIRGFNGSKQYFPTYEQLGIQSTDTFYLKGIA
                     KMYLAKSELRFPIYGNFGGAVFYDGGAVHIERHDLGDEYRDATGFGFHYNTPVGPVSL
                     EIAWKLDMKPNEDPYAVHLSIGSF"
     gene            complement(3557..7570)
                     /locus_tag="VIG33_04345"
     CDS             complement(3557..7570)
                     /locus_tag="VIG33_04345"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEY8270093.1"
                     /translation="MKRVLLILLPPLIFLILLMGTLKYWVLPHTEVWALQKLKTYSEA
                     NLPVVVEAERIQFHILKPAASIEKITITAKKDLQKFTDQMNIESIKVNLDLFQLLAGR
                     VQISAILVDSPEGHIQLDSILDSSSKPEPLPLNLLFELLEKIPVERAFVHNLQLELQS
                     QKHQSSARLQGADLLLGNHQNSLTARLDIPRLSLVAMGHGPLEARFDISAVLGRDTLR
                     ITRVGARYGDSEVIVKGEFSKFSQIALHPAATLEAMGNLKLQEIIRQLRPIFPEHKLP
                     LLNGEAIAKMNIKITGKAADKTAEKTTDSIQITGKIDLKTKNVSLDSFTLGNASIQGL
                     FKGDRITFPEVQINHPAGNARLLNTDIQLNDKIEFKTQATVSKLDLQKLFLSLNLKAI
                     PVWMNLQGELPCEGQIKPPLKILCNANLSAENLVVHSDYHNKKSVIADISKFGAKGQT
                     EITDKGVSYKAQVSLGNNLGESSGIIDFAKGFKINYSSPRVDFSNIRNLANLKFKGYG
                     SIEGSTSGDSHAAVFDMKLKMQEFSFEDYYLGQLNGLLAYERGHLLLNDLNGFLPKSG
                     YQGSLDVNLTDANAKIVGRIKAPTLDLEDLVKVFANQYHFPVSVKGPGWAEVAFSGPL
                     DFWKLSYQLDSKFKGGQFAGESFDELVLSAQATEGTLQIQKASVKKNQASILATGGIS
                     SQQDLNLKMEGRNFRLDESDFVSKISSNIFGILNFTSQLRGKVTEPEISIRGSITETV
                     FDEQETPSSFFDLKIRKTVMEGNANLFGHRIQADWLIPFNNSPLRLRLKTVDWNYASL
                     LSVLSGNTYQNEYDSSLTTEVDLQSESGLWQKASGLIQVKNLFLKRGSLSFRNPDPIQ
                     IKIDSGKIRIENFNLEGPQNSIQLQGQDFTLNNLNMNLVANTELRLFHMFFPFLDDFG
                     GSVQTAATLSGSLFKPQILGNLTTHNSFVKIKGFPHPIERIQAEVNFSQTKILINSLK
                     AQMAGGLVTADGNIIIKEYRDIPTSIRMNLEGVHLNIPDKVHTFGSADLLLSGKWFPF
                     LLSGTYKVASGIFEREFTESGETSLNNSRQSIYLPKVLRQSSFEPLILDIQILLEKSF
                     LVKNSLVDGSVQGNLQVKGSTSNPLLLGRLQTDKNTKLIFKDKAFEVQTANVLFNNPD
                     EINPELYVSAQARVNDYDIMILAQGPAKNLKSIRLTSIPPLPEQDIISLLALGVTSSR
                     SDTTAQGREQSVQAGYELGFAIMSQPINKQLQDRLGLNVQFTSSFDTTRNISVPKVTF
                     SRKLSHKMDASASRTLGSDVNDVEVKLRYLINQNVSAIGSYENSQTLQGGQGLTNTGR
                     QKENVFGLDLEFRKEFK"
     gene            complement(7567..8616)
                     /locus_tag="VIG33_04350"
     CDS             complement(7567..8616)
                     /locus_tag="VIG33_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270094.1"
                     /translation="MTLSLELEIKALLEEFRDPEIVAEKLIRLFEEHSDEWSEKSVKE
                     LSSFLLSCNLYQQLIAFCLKHLRSKKFVMPWPYFIQAVALGIPDLDEDVVKLIILGIE
                     EEKAMEQASLGQGAERFIKKALQWKNEFKRKRRKYASEIKQKMLDELLTLRTQQLYEQ
                     EKIMLGKMQRMFPGDQDVIEELSLHKERYALDILSRRSSLGRRIHQVQDASSSPEVQA
                     ATDALKESLLEAVKIYPEMAFDFAIAAFMLDEPQISLALLETAELTLDQHWFYLEVKL
                     KCRHFLEVLTALSALELQLAHEPETFFATAYLRAQAYWGLEQKYVAIEILETILEARP
                     NYRASSTLLDLWRSQ"
     gene            complement(8775..9341)
                     /gene="efp"
                     /locus_tag="VIG33_04355"
     CDS             complement(8775..9341)
                     /gene="efp"
                     /locus_tag="VIG33_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEY8270095.1"
                     /translation="MYETGDFKKGLKIMVEGKPYVIVDFQHVKPGKGNQFSRTKLRNL
                     LTGQNLEVTFKSGEKFEVPNVENKEMTYLYKDDSGYSFMDQESYEQLTMNEEELGDTK
                     YYLVENLKVVILFYNEKAVAVDVPKAVNLKVAKSDPGIKGDRVSGATKPATMDTGLVV
                     NVPLHINEGDVLRIDTESGAYVERVNSK"
     gene            complement(9402..10247)
                     /locus_tag="VIG33_04360"
     CDS             complement(9402..10247)
                     /locus_tag="VIG33_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8270096.1"
                     /translation="MPKIEASAIEKYQMILQKDANSQVFAPLADAYRERGMLKEAEQI
                     VSAGVKRHPSFASGFVVYARIMKDLSKPEESLKLIRKAVELAPENIMAHHLQAEIYLQ
                     SKRPKEALKAFKMVLFLNPQSERARKAVAKLESLTADEYEDDVFEMSKIASLKEQFWT
                     EDEPEEEPRNEKEVTKTSSPPSPSPQGLERTISLIDALIVRSDLTKAKMLLTDAQSEF
                     GDHPEILQRLKLLHSRSGPSMAASDGGQATPLKPLVSREKAIQQRKLEVLEMMLRNIG
                     EYRQL"
     gene            10330..10839
                     /gene="ruvC"
                     /locus_tag="VIG33_04365"
     CDS             10330..10839
                     /gene="ruvC"
                     /locus_tag="VIG33_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY8270097.1"
                     /translation="MSYLILGVDPGSRITGFGLVRVQRGGCLEHVSHGTIVMDVEGEF
                     PQRMMELGSAFSEIMQKYRPNHVAIEKIFLGKNADSAFKLGHARGVAMYESIRGGAQV
                     FEYATRLVKKGVTGNGGASKEQVQAVLKSLLSLRKIDRLDASDALAMACHHAYEVQRQ
                     RLVRRAIEI"
     gene            10872..11456
                     /gene="ruvA"
                     /locus_tag="VIG33_04370"
     CDS             10872..11456
                     /gene="ruvA"
                     /locus_tag="VIG33_04370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEY8270098.1"
                     /translation="MIGFLRGILVEVAADVALMDVNGVGYEILASGNTLSDLQTFIGK
                     EIIVWIHTHVREDAFQLFGFHSKEEKNLFLSLLKVNGIGPKMALSILSGARVEQIYEM
                     IESSNAKALSCLPKVGKKTAEQMILTLKGKLVLLEKSDAKKKASGTLLEITSALLNLG
                     FKPQAVEQFISTLPQDADLEEGVRRGLAALAQPL"
     gene            11530..12531
                     /gene="ruvB"
                     /locus_tag="VIG33_04375"
     CDS             11530..12531
                     /gene="ruvB"
                     /locus_tag="VIG33_04375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEY8270099.1"
                     /translation="MGRILEGDPAEGEKSWENVLRPQNFEDFPGQHEVKDKLKIFVSA
                     AKGRGEALDHTLLSGPPGLGKTTLAKIIANDLGADFKATSAPAIDKKGDLAAILTSLK
                     PQAVLFIDEIHRLSRHVEEYLYTAMEDFFIDIVTGDGLGARSMKFQLAPFTLIGATTR
                     AGLLNAPFRDRFGIVERLHFYDKESLQQILERDAKLLNIALEPKGAAEISRRSRGTPR
                     VAIRLLKRVRDFAQVQGNGLITQDLANSALNKLGVDRFGLDLMDRRILQLIHEKYAGG
                     PVGVETIAAALSEERETIEEVYEPFLLQEGFLQKTQRGRVLTEFAKNQILDSSLSLL"
     gene            12649..14511
                     /locus_tag="VIG33_04380"
     CDS             12649..14511
                     /locus_tag="VIG33_04380"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEY8270100.1"
                     /translation="MKLIKNHHGTSLVTVLMATGIGAIILAGITTMITDVFKSQRTAQ
                     SKDAHRELTVSIRQLLTDPVICSASFKGEDPAGTGFTRTQIVDAATPPNIKYQTGVNY
                     LNNLVTITGFEVRGFVADNPTVNDKIGKAELNIKMNKLGSTIGSPQMTTTVFLQATLN
                     AADTNLNSCYALGLADSLWQISPANMADIYYPSGNVGIGIGNPGDLLHVNGSISSTAS
                     ATGAIPTSIRLDYCGNCGTAGGGASRISAIGADVANRGEFRIDQYSTDATNHYVPFFI
                     NRSGNVGIGTTAPRMGFDVIVPVAQGASSFGAQGSTKAVAAGIPPARPNPTVYGWDFS
                     TSNHVPLELRAEPGPQLYLDTNGYIGIGRTAPLANLHIMSNNAPARLAIHNPQEESVF
                     LELAGTGGVGGAEGFEIEYQNNGGDTFLRNRYPIGNVYFQTSTASGAPKTNMTLSYTG
                     NVGIGLITPNYLLDVGGDINASGSVRASGVALSSDVRFKRDIHTLDNVLGKILKLRGV
                     SYEWRSEEFPEKHFTTRPQIGVIAQEVEQEFPELVDTDKNGYKSVNYPALVSPLIEST
                     KELYGMCKANEGQIKEISRKLASVENKASKLEAENALLKARLERIERILQSPSK"
     gene            14672..>15016
                     /locus_tag="VIG33_04385"
     CDS             14672..>15016
                     /locus_tag="VIG33_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270101.1"
                     /translation="MKTPRSTLLTPPRKAFLRQLILGLSSVFLIMEPALAATSGFTYS
                     GKILDSNNAPVTATAVIFTVTVFDPAGKCWLYTEQRKLDLSQTAGTFSFEIGSSDIST
                     LYGAPPSFNNQSS"
BASE COUNT         4015 a         3230 c         3515 g         4256 t
ORIGIN      
        1 gtcatttttc cttgggatat caatacgagt gtctccatta gagagtgtgt actgaattcg
       61 aacccaatta gagtgctcac gcacgtcaga atcgtatcca acacaaacgg tttcaatttt
      121 aactggttca aatattttaa aacgggtgtt ccggccgcaa tacatgggaa caaaggtgtc
      181 agacgcgagt gcagcattgg atgccgcaat aatagccaat aagacggtgg tcactaactt
      241 tttcataaaa tctccttagg gttggagctc tgattgagct cattgttatg ggcttataat
      301 aacgcaatat ataaattata aaacctagaa aaatggcacc gtcgttgttt gtgctaaggt
      361 agcgataata ttaatgatat aaatatgttt atattcaaaa atagcataaa aatgttatcg
      421 gtatttatta ttcgcaggag gaaggctgtc tgatagactc ctggcttttt tttggttttg
      481 taaagttaac aagcctggtc ctttgctgga cttgaactta gggactatcc atcaaagtat
      541 agcgccaaat aatgttgtag ggattattct acagaatgca tgggccctaa cggagagcat
      601 ttgtacctaa gagcagcaga cttaaatcta tttttctagg tctagttggg ttaactttac
      661 ataacccaaa aagcaacgcc atctttggac tttatgttaa catcaagacc gtcaaaagcc
      721 ttccctagaa cacccaatct ttagaagctg ccgatggata agtgaacggc atagggatct
      781 tcattcggtt tcatatcaag tttccaggcg atttcaaggc tcaccggacc caccggggta
      841 ttatagtgaa agccaaaacc tgtggcgtcg cgatactcat cgcccaaatc atgacgttca
      901 atatgaacgg ctccgccgtc atagaagaca gcgcccccga agtttccata aatcggaaaa
      961 cgcaattctg attttgccaa gtacattttt gcgatccctt taagataaaa ggtgtcggtg
     1021 gattgaatgc ctaattgctc ataggtggga aaatactgtt tggatccatt gaagcccctg
     1081 atggtcgaaa tgccgcctaa atagaagccc tttttatcat aaggaacccc atcagtggct
     1141 ccactttttt ggctgagatt ctcaagatag cccccccgaa tagcgttggc ccaaatcata
     1201 aagttatctt tgagataggg gagtcgcata taatgagtga aagagcctgt gactctttgg
     1261 aatttgatct ccccttcgcg ggaactgcca atgtccggtg aagagtactc ataagaaagt
     1321 gtggtgaagt ggcccttggt tggattgaag ggattatcac gataatcgat gtccaagcgt
     1381 ggtcccgtgg tcacgatatc ttgaggggct ggcgttggcg ttccaggggc gggatgtaag
     1441 aagtaatcct gaagatgggc gctattccaa agttcataaa taactgtgat atgtgaggtc
     1501 aagtctcttt ccaaggacca agtggtctgt ctgacatcgg aaactttttt agtggtgtaa
     1561 tcagagagca gactggagcg ggtgaggttc acacggccgc gaattttgga gtcaaataaa
     1621 taaggttcga catatcccaa ggtcactttg gattcgaggt atttgatttc gttgacattg
     1681 taatttccct caagacgtaa agaaatccct cgtcccgttc ccataagatt attataagcc
     1741 agaccggtgt agcctctcag ggtcaatccc agatcattat tggcacctgc tcccaaagta
     1801 aaaaggcccg ggtctctttc gctgaccttc accaccaacg tacgattggc aacgctggtc
     1861 ttttcttcga gggttttaac atcgacggta ttaaagtagc ccgtgcgttg cagttttgcg
     1921 atggcctcgt ccaatttacc aggcgtcacg gtgtccccgg tttgcaggtc aatttctttt
     1981 aagagcacat aatctttggt gaaagtattg ccatccaaaa taatattagc aatacgaact
     2041 tgggggcctt catagatctt gaaattcaac ttcgccagag tgttgttgtc gttataggag
     2101 acaaggtctt ctttttcatt cagaagagtc atgtcaatat agcctctttc ttgataatat
     2161 attttcaaac tttgaatcgc cttttctaac tgggctagac gcaaagtctg ttggctggcc
     2221 agatcaatgg cttttaaaag ctccgctgcc gggacggttt cattgccagt gaattgaatg
     2281 ctttcgattc tagtgagagg tccttcatcg aggttgacat ggacagagac ttggtttttt
     2341 tccttgttgt actgagtccg tgtggaattg attcgagcca aaagaaaacc ctgattttgc
     2401 aattcaatac gcatcgcttc gaaactttga tccagttcgg ccttgttata gtaacctttt
     2461 tgaatcaact ccgaactttg tttttttagc aggtttacat aaaaatcgga aggacgcgaa
     2521 atgttgcccg aaacctcaaa tttcgctatt ttaaccctgg gaccctcatc gatattgaaa
     2581 acgattttac gagtgaaggg cttacgacct tcgaggtctt cgctgcgcac ctctactctg
     2641 gcgtagccac gggataaata aaggttttta attttggtcg agagttccgc gccaatactt
     2701 ggattcgcgg aatagaaatt tcctatatcc aaagaattat tcaaagcgga agaggagatt
     2761 tcgcgattgc ccgtatactc aataatgtag gaatccactt tctcgagggt cagggttaat
     2821 tgggccttgg actcgttggc attgaaagtg atttccggtt ctttgatttc cgtgcgtaga
     2881 taaaatttcg cattcaaata gtcacggatt tttttaagta tttccgcaag ctggacatcc
     2941 gtcagcgcat aacctctttc tcgggacaag cgattttcca gagctttgtt gagatccgta
     3001 tgaggggagt ttaaattgat ggcgccaatt aaagtttgga cgccttcttt gactttcacc
     3061 agcagttgga catcaccttt tccatccgga ggaatctcga cgtcaatgga ggtattccta
     3121 aaaccaattt gattgtactg ctggcgtagt ttttttccgc cattgatcaa ctgatcctga
     3181 ttcaggactt cattgaggtt gataccgaaa agttgtttcg cttcggattc agaaatcgcc
     3241 cgcagacctt caaacttaat ggcgctgact cgagtcgcac gttccgtatt gagtgttaag
     3301 tttccgttgc ctaggtccca cgcttgtacc aggtcaaatt ccggacgaag ctgtaaaaag
     3361 cgaataattt catcgatggt ttctaaagtg attttattgc ccgtgacttg gggaaaacgc
     3421 agaaccaatt ctttttgaat cgcgggcgaa agggcagaaa tatttaaact tcgatgaacc
     3481 acgcccgctg cccagattaa ttgtgcggcg aaagaaaaac taaaaagaaa agttcgcaga
     3541 aaagatttca atttcactat ttgaattcct ttctaaactc gaggtccaag ccaaaaacat
     3601 tttctttttg acgtccggta ttagtgaggc cttgcccacc ctgaagagtc tgactatttt
     3661 cgtaactgcc aatggcggaa acgttttgat tgattaaata acggagtttt acttccacat
     3721 cattcacatc tgaacccaga gtacgacttg ccgaggcgtc catcttatgg gatagcttac
     3781 gactgaaggt gactttaggc acgctgatat ttctggtcgt atcgaaggaa gaagtaaatt
     3841 gcacgttcag acctaaacga tcttgcaact gcttattgat tggttgagac atgattgcaa
     3901 aacccaattc gtatcctgct tgaacggact gctcacgccc ttgagcggtg gtatcagaac
     3961 gcgacgaagt gactccgaga gccaacaaag agatgatatc ttgttccggc agcggcggaa
     4021 tgctggtcag gcgaattgac ttgagatttt tagccgggcc ctgggccaag atcatgatgt
     4081 catagtcatt cactcgggcc tgggcggaaa catagagttc gggattgatc tcatctggat
     4141 tattgaaaag gacattggct gtttgaacct caaaggcctt gtctttaaaa ataagtttgg
     4201 tatttttatc cgtttgcaga cgtcctaaaa gaagggggtt tgaagtcgaa cccttcacct
     4261 gaagatttcc ttgcacagag ccgtccacca gggagttttt aaccagaaag cttttctcca
     4321 agagaatttg aatgtccaaa atcaaaggtt caaaactgga ttggcggagg acttttggca
     4381 aataaatact ttgacgggaa ttattaagac tggtctcgcc gctctcggtg aattctcttt
     4441 caaagattcc actggccact ttgtaagttc cagaaagcaa gaagggaaac cattttcccg
     4501 aaagaagtaa atcggcggat ccaaaagtat ggactttgtc tggaatattg aggtggacgc
     4561 cctcaagatt catgcgaatt gaagtgggaa tatcccgata ttccttgata atgatattac
     4621 catcagcggt gactaaacct ccggccattt gtgctttgag cgaattgata agaatttttg
     4681 tttgggaaaa attcacttcc gcttgaatgc gttcaattgg atgcggaaag cctttgattt
     4741 taacgaagct attgtgggtc gtgagatttc ccaagatctg tggtttaaat aaggagcctg
     4801 acaaagttgc tgcggtttgt accgagcctc caaagtcatc caagaaggga aagaacatat
     4861 gaaacaagcg aagttcagta ttggccacca agttcatatt gaggttatta agggtaaagt
     4921 cctgtccttg aagttgaatg ctgttttgtg ggccttccaa attaaaattc tcaatgcgta
     4981 ttttacctga gtctattttt atttgaatag gatcggggtt acggaaactc aaagaaccgc
     5041 gttttaagaa caaattcttg acctggatga gaccactggc tttttgccac agaccacttt
     5101 cagattgcaa gtccacctcc gtcgtcaggc ttgagtcgta ttcattttga taggtgttgc
     5161 cactgagaac tgataacagt gaagcataat tccaatccac cgttttgagt cgcaagcgca
     5221 gtggcgaatt gttaaaggga atcagccaat cggcttgaat gcgatggccg aataaattgg
     5281 cattgccctc catgacggtt ttgcgaattt taagatcaaa gaaagaagac ggcgtttctt
     5341 gttcatcaaa tacggtctcc gtaatgctgc cgcgaattga aatctcaggc tccgtgactt
     5401 tgccccgcag ttggctggtg aaattgagaa tgccaaaaat attcgagctg attttagaaa
     5461 caaagtcaga ctcatccaga cggaaattac gcccttccat ctttaaattc aaatcttgct
     5521 gtgagctaat tccaccggtc gctagaatac tggcttggtt ttttttaacc gaagcctttt
     5581 gaatttgcag agttccctca gttgcttggg cactgaggac caattcgtca aaagattcac
     5641 cggcaaattg accgccctta aacttagaat ccaattgata agataatttc cagaaatcca
     5701 agggacccga aaaagcgacc tccgcccagc cagggccctt cacggaaaca gggaagtggt
     5761 attggttggc aaagaccttc acgagatctt caaggtccaa agtcggagct ttaattcgtc
     5821 ccacgatttt tgcattagca tcagtaagat tcacgtccaa agatccctgg taaccggatt
     5881 ttggcagaaa gccattcaga tcgttgagga ggaggtgacc gcgctcatag gctaagagtc
     5941 cattgagttg gcccaggtaa taatcttcaa acgagaactc ctgcattttt aatttcatat
     6001 caaagaccgc tgcgtgggaa tcaccagagg tgctgccttc gatgcttcca tagcctttaa
     6061 acttgaggtt ggccagattg cggatatttg agaaatcaac tctaggagag ctataattta
     6121 ttttaaatcc cttggcaaaa tcaatgatgc cagaactttc tccgaggttg ttccccaaag
     6181 agacctgggc tttgtaactc acgcctttgt cagtaatttc cgtttgacct ttagcgccga
     6241 atttggagat gtccgcaatg actgattttt tattgtgata gtctgagtgg actacgaggt
     6301 tctcagcact gaggtttgca ttgcaaagga tttttaaggg cggtttgatt tgcccttcgc
     6361 agggaagttc accttgcaaa ttcatccaca ctggaatggc tttcagattt aaagaaagaa
     6421 aaagtttttg cagatcaagc ttggaaacgg tggcttgagt tttaaattcg attttatcat
     6481 tgagttggat atcggtattc agcaagcgag cattgcccgc cggatggttg atttggactt
     6541 cggggaaagt gatgcgatcg cctttaaaga gtccttgaat agaggcgtta cctaaagtga
     6601 aagagtccaa agaaacattc ttggttttta aatctatttt tcccgtgatt tgaatcgagt
     6661 cagttgtttt ttcggctgtt ttatcggcag ctttgccagt gattttaata ttcatcttgg
     6721 cgatggcttc gccgtttaaa aggggaagtt tgtgttccgg aaagatcggt cgtagctgtc
     6781 gtatgatttc ttgaagcttt aaatttccca tggcttccag agtcgcggcc gggtgaagag
     6841 cgatttgcga gaacttactg aattctcctt ttacgatcac ttcagagtcg ccgtaacgag
     6901 cgcccactcg ggtgatgcgc aaagtatcgc gtcccaacac cgctgaaata tcaaagcgag
     6961 cttccagggg cccgtggccc atggccacta aacttaagcg ggggatatcc aagcgcgccg
     7021 tcagagaatt ctgatggttt ccgagaagga ggtcggctcc ttggaggcgc gcggaggatt
     7081 ggtgtttttg agattgaagt tctaactgta agttatggac aaaagcacgc tctacgggga
     7141 ttttttccag caactcaaag agcaaattta aaggcaaagg ctcgggcttt gaggaactgt
     7201 ccaaaattga atccaattga atatggcctt caggggagtc cactaaaatc gccgagatct
     7261 gcacacggcc cgcaagaagc tggaacagat ccaagtttac tttaatgctt tcaatgttca
     7321 tttgatccgt aaatttttgc agatcttttt ttgcggtgat cgtgatcttc tcaatactcg
     7381 cggcgggttt taaaatgtgg aactggattc tctccgcttc caccaccacc ggcaggtttg
     7441 cttcagagta ggttttaagc ttttgcaaag cccaaacttc ggtatgtgga agaacccagt
     7501 attttagagt tcccattaaa aggatcagaa aaatcaatgg gggcaaaaga atcaggagca
     7561 ctcgtttcat tgcgatctcc acagatccaa gagggtggag cttgctcggt agttcggacg
     7621 tgcttctaaa atagtttcaa ggatttcaat ggctacatac ttttgctcaa ggccccaata
     7681 ggcctgggcg cgtaaataag ccgtggcgaa aaacgtttcg ggctcatggg ccaattgcaa
     7741 ttcgagtgcg ctcaaggcgg tcaacacttc taagaaatgg cgacatttta atttcacttc
     7801 caaataaaac caatgttgat caagggtcag ctccgcagtt tccaacagcg ctaaactgat
     7861 ctggggttca tcaagcatga aagcggcgat ggcaaagtcg aaagccattt ccgggtaaat
     7921 tttgaccgct tccaagagag attctttcaa agcgtcagtg gccgcttgga cttcaggaga
     7981 agatgaggcg tcttgaacct gatggatgcg acggcctaag gaggatcgcc gcgacagaat
     8041 atcgagtgca taacgctctt tatgcagact gagctcttca ataacatctt gatcccctgg
     8101 gaacatccgc tgcatctttc ccagcatgat tttttcttgc tcgtaaagtt gttgagtgcg
     8161 caaagtaaga agttcatcga gcattttttg cttgatctca ctggcatatt tgcggcgctt
     8221 gcgcttgaat tcgtttttcc attgcagagc ttttttaata aagcgttcgg caccttggcc
     8281 aagcgaggcc tgttccatgg ccttttcttc ttcaattcct aggatgatta atttcaccac
     8341 atcttcatcc aaatctggaa ttcctagagc caccgcttga atgaaataag gccaaggcat
     8401 cacaaatttt ttagagcgga gatgtttcag gcaaaatgcg atcaattgtt gataaaggtt
     8461 gcaagacaac aggaaactag aaagttcttt cacgcttttc tcagaccact cgtcagaatg
     8521 ctcttcgaaa agccgtatta acttttcagc cactatttct ggatcgcgaa attcttccag
     8581 cagagcctta atttccagtt caagcgaaag cgtcaaaaac agcccccaaa atccgttttg
     8641 gatcaaccct cttattttca gccattttgc agtgaatctc gaggcttaac gcatattgcc
     8701 attcaaaatt gataacaaaa aaaagccctg aagcttcaca gttcaaggct aaagtcataa
     8761 tttaagaaga gtttttactt cgaatttacg cgctctacgt aagcaccaga ctctgtatca
     8821 atgcgaagga catctccttc attgatgtga aggggaacgt tgaccacgag tccagtatcc
     8881 atcgttgcag gcttagtagc cccggaaaca cggtctcctt tgataccagg atctgatttt
     8941 gccactttca agttgactgc tttcggtacg tcaaccgcaa cggctttttc attgtagaat
     9001 aaaatcacaa ccttcaagtt ttctacaaga tagtattttg tatcgcccag ttcctcttcg
     9061 ttcatcgtca actgctcata ggattcttgg tccatgaagc tgtagccgct gtcgtccttg
     9121 tagaggtaag tcatctcttt attttcgacg tttggcactt cgaatttctc acctgatttg
     9181 aaggtcactt caagattttg gccggtgaga agatttctca gtttagtgcg tgagaactga
     9241 ttgcccttgc caggttttac gtgttggaaa tcaacgatca cgtacggctt tccctcaacc
     9301 atgatcttaa gacctttttt aaaatcccct gtttcgtaca tagcatcccc gtgtgaaata
     9361 atgaggagcc aatataagcc aaaaaggcgg gggctggtca attaaagctg ccgatactca
     9421 ccaatattgc gaagcatcat ttcaaggacc tcaagttttc tttgctggat cgctttttcc
     9481 cgagagacta aaggtttcaa aggggtggct tgtcctccat cggaagccgc cattgagggg
     9541 ccgcttcggc tgtggagaag tttgagcctt tgtaaaattt ccggatgatc tccaaactca
     9601 gattgagcat cagtcaatag catttttgcc ttggttagat cactgcgtac aataagagca
     9661 tcgatgaggg agatcgttct ctccagcccc tgaggggacg gagacggagg cgacgaagtt
     9721 ttagtgactt ctttttcatt ccttggttct tcttctggct cgtcctcggt ccaaaattgt
     9781 tctttgagag aagcaatttt actcatttca aaaacatcat cctcatattc gtccgccgtc
     9841 aagctttcga gtttcgccac ggctttgcgg gcacgttcac tttgcggatt caaaaataaa
     9901 accatcttaa aagccttgag ggcctctttg ggacgtttgc tttgtaaata aatctctgct
     9961 tgaaggtggt gggccatgat attttcaggt gccagctcca cggctttgcg tatgagtttc
    10021 aaactctcct cgggcttgct caaatccttc atgatccgcg cataaacgac aaagcccgaa
    10081 gcaaacgagg ggtgacgctt cactccagcg ctgacaattt gttcggcttc tttcagcatc
    10141 cccctctcgc gataggcatc cgccaaggga gcaaagacct gagagttcgc gtctttttgc
    10201 agaatcattt gatatttttc aatagcgctt gcttcgattt tgggcatgga tttatcatga
    10261 catccccact ttataatttc aacagagctt tttattgacc tccttgcggg gacgaattca
    10321 aatacgctga tgagttatct aattttaggc gttgaccccg gttcacgcat cacaggattt
    10381 ggtttggttc gggttcagcg aggtggatgc cttgagcacg tgagtcatgg gactatcgta
    10441 atggatgtgg agggggaatt tccccaacgc atgatggagc tgggcagtgc tttctctgaa
    10501 atcatgcaaa aatatcgccc gaatcatgtc gccattgaaa aaatattcct gggtaaaaat
    10561 gcggatagcg ccttcaagtt gggacatgct cgcggcgtag cgatgtacga atccatccgt
    10621 ggtggtgctc aagtctttga atacgccacg cgccttgtaa aaaaaggcgt gacaggaaac
    10681 ggcggagcta gcaaagaaca ggtgcaagct gtattgaaat ctttgttaag tttacgaaag
    10741 attgatcgtc ttgatgcctc cgatgccttg gcaatggctt gccatcatgc ctacgaagtt
    10801 caaaggcaac gcctggttcg tcgcgccatt gaaatataaa gcgttagaaa aaatctaatt
    10861 taagaggtct tatgattggt tttttaagag gaattttagt agaggttgcg gctgatgtgg
    10921 ccctgatgga cgtgaacggc gtgggttacg agattttggc ttctggcaat actttatccg
    10981 acctgcaaac atttatcggc aaagaaatca ttgtatggat tcatactcat gttcgtgaag
    11041 atgctttcca acttttcggc tttcattcca aagaagagaa aaatttgttt ttatcacttt
    11101 tgaaagtcaa tggcataggg cctaagatgg ctttgagcat tttgtccggt gcacgcgttg
    11161 aacaaattta tgagatgata gaaagcagca atgccaaagc gctttcctgt ttacccaagg
    11221 tagggaagaa aacagccgag caaatgattt tgactcttaa aggaaaactg gttctcctcg
    11281 aaaaaagcga tgcaaagaag aaggcttctg gtactttgct agagatcact tcggccttat
    11341 tgaatttggg ctttaagccg caggctgtgg aacaattcat ttcaacgctt ccccaagatg
    11401 ccgacttgga agagggagtt cgccgtgggt tggcagcatt agcacaaccc ttatagaaat
    11461 cagaaattag agtaaaaata gaaatagagt aaagaagaag agtaaagaag aagagaagat
    11521 ggagtgggtt tgggacgtat tttagagggt gacccggcag agggtgaaaa atcttgggaa
    11581 aatgttttaa gacctcaaaa ttttgaggat tttccgggac agcatgaggt gaaagacaag
    11641 ctgaagattt ttgtgagtgc tgcgaagggc cgtggcgagg ctttggatca tactctgctg
    11701 agcggtcctc cgggacttgg taagaccacc ttagcaaaaa ttattgccaa tgatttgggt
    11761 gcggatttta aagccacctc tgcaccggcc attgataaaa aaggggatct ggctgcgatc
    11821 ttaaccagcc tgaaacctca agcggtttta tttattgatg agattcatcg actgagtcgt
    11881 cacgtggagg agtatcttta caccgcgatg gaggattttt ttatcgacat cgtgacagga
    11941 gatggactgg gggcccgctc aatgaaattc caattagcgc ccttcacttt aattggagcg
    12001 acgacgcggg cggggctttt gaatgctcct tttcgagatc gttttggaat tgtcgagaga
    12061 ttgcattttt acgataaaga gtctctgcaa caaattctcg agcgtgatgc taaacttctg
    12121 aatattgctc ttgagcccaa gggagccgca gagatttctc gtcgctctcg gggaactccg
    12181 cgggtggcta ttcgtttgtt aaagcgtgtg cgcgatttcg cgcaagttca aggcaacggt
    12241 ctgatcactc aagatcttgc caactccgcg cttaataaat tgggcgtgga tcgcttcgga
    12301 ctggatctta tggaccgtcg tattcttcaa ctgattcatg aaaagtatgc tggaggcccg
    12361 gtgggggtgg agaccattgc cgcagcttta agtgaagagc gcgaaacgat tgaagaggtt
    12421 tatgaacctt tcctattgca agaaggcttt ttgcaaaaga cccaacgagg ccgcgtcctg
    12481 acagagtttg caaaaaacca aatcttggat tcttcactga gcctcctttg attcagattt
    12541 taggctgggt gcgtgtctta ttttgaggct cgtgtctatg attatctgta ttgatctatt
    12601 ataaattgtt tacgttaagt atctttttct ttctgtcgat atttaagtat gaaattaatc
    12661 aaaaaccacc acggcacgag ccttgtcact gttctgatgg ccactggcat tggcgccatt
    12721 attttagctg ggatcacgac catgatcaca gacgtgttca aatcccagcg cacggcccag
    12781 tctaaagatg cccaccgaga actgacggtc agcatccgtc aacttttaac agatccggtt
    12841 atttgttcgg caagcttcaa aggagaggat ccggcgggga ctgggtttac aagaactcaa
    12901 atcgtggatg ccgccactcc ccccaatata aaatatcaaa ccggagtgaa ttaccttaat
    12961 aatctcgtca ccatcaccgg gtttgaagtc aggggtttcg ttgcagataa tcccactgtt
    13021 aacgataaaa taggaaaagc tgaactcaac atcaagatga ataaactggg ttccaccatt
    13081 ggcagccccc agatgacgac gactgtgttt ctgcaggcga ctttaaacgc ggccgatact
    13141 aatcttaata gttgttatgc cctggggctt gcggattcac tgtggcaaat ctcgcctgcg
    13201 aatatggctg atatttatta tcccagtggc aacgtcggta taggaatagg aaatccgggt
    13261 gatttgcttc atgtgaatgg ttcaatttct tcaacggcat cagcgacggg ggctattcca
    13321 acttccattc gactcgacta ttgcggcaac tgcggcacag ctggcggggg agcgagccgc
    13381 atctcggcca ttggcgctga tgtcgcgaac cggggcgaat tccgtattga ccagtacagc
    13441 actgatgcta cgaaccacta tgttccattt ttcattaacc gctccggcaa tgtcggcatt
    13501 gggaccacgg cgccgcgaat gggctttgat gttatagtcc cggtggcgca gggtgcttca
    13561 tcttttgggg cgcagggctc gacgaaggcg gttgctgcag gaattcctcc cgcacgacca
    13621 aatcccaccg tttatggttg ggactttagc accagtaatc atgtgccgtt ggagttacgt
    13681 gcggagccag gccctcaact ctatttggat accaatggct acattggtat agggagaaca
    13741 gctccattgg caaatttaca tataatgagt aacaatgccc ccgccagact tgcgatccat
    13801 aatcctcagg aagagagtgt ttttctagag ctggctggta cgggaggtgt cggaggagcc
    13861 gagggctttg aaattgagta ccaaaataac ggtggcgata cttttctacg caatcgttat
    13921 cccataggaa atgtttactt tcaaacgagt acagcatcgg gtgcccctaa aacaaatatg
    13981 accttatcgt atactggcaa cgttggcata ggtcttatca cgcccaacta cctgctcgat
    14041 gtgggcggag atatcaatgc atccggtagt gtgagggctt ctggtgtggc cctcagttct
    14101 gatgtcagat ttaagcggga tatacataca ctagataatg ttcttggaaa aattttgaaa
    14161 ctccgagggg tcagctacga atggcgaagt gaagaatttc ctgaaaagca tttcaccact
    14221 cgcccgcaaa tcggtgtcat tgcccaggaa gttgaacaag agttccctga actcgtggat
    14281 actgataaaa atggctataa gtccgtgaac tatccagcac tagtttcccc cttgattgaa
    14341 tccactaagg agttatatgg catgtgtaag gccaatgagg gtcaaatcaa agagatctcc
    14401 cgcaagcttg cctctgttga gaataaggcc tctaaactcg aagctgagaa tgccctactc
    14461 aaagcacggc tggaacgaat cgagagaata ctccaatccc cttccaaata gctctggaaa
    14521 aactgaaact ctggtttgat aaactcaaac gagttttttt cgaaccttca aacgatctcg
    14581 gggataattc aattgtttta aagaaggatt taccagctaa ttagcgtcaa aaaactaaag
    14641 aaaatgggta ttttttccga tattttagaa tatgaaaact ccccgtagta cgttactaac
    14701 acctccaaga aaggcctttt taaggcagct tattttaggg ctgtcttccg tattcttaat
    14761 tatggagcct gcgctggccg cgacttcagg ctttacttat agcggtaaaa tcttagactc
    14821 caacaatgct ccggtcacgg ccactgcggt gatctttaca gtcacggtct ttgaccccgc
    14881 cgggaaatgc tggctttata ccgagcaaag aaaattggat ttatcccaaa ccgccggaac
    14941 cttttccttt gaaattggct ccagcgacat ctcaactctt tatggagctc ctccttcatt
    15001 taacaaccaa tccagt
//
LOCUS       DATBTE010000118        13095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_834752, whole genome shotgun sequence.
ACCESSION   DATBTE010000118 DATBTE010000000
VERSION     DATBTE010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 13095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13095
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834752"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..769
                     /locus_tag="VIG33_04390"
     CDS             395..769
                     /locus_tag="VIG33_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270102.1"
                     /translation="MKKLICLALMLSSVNSFANTYSGVTLEDGYPCSLEMGFSAGFIA
                     NFRYRNFPVGHGEVTSQVQREYERFMSGGGGEGSSNFTLKDPSTGNSINVTHRNSDLK
                     LVSATLAKEGRFITCEIKSWRP"
     gene            1226..1996
                     /locus_tag="VIG33_04395"
     CDS             1226..1996
                     /locus_tag="VIG33_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270103.1"
                     /translation="MEVSKDPLDEGYKLIDQGKYSEAIQELQSLSLRDFRPSVKVALA
                     SAYAARGDIRVEHYWGFVIGFATPLVSSESSLESSHIESLQKIIKETHHRDANRQDLQ
                     ALEDLMEAATVWDQYKNRLDAIPSVSGEALMDLKSAVDILGGVQTPGGRLYRAILNLI
                     LFKSYITASGGFWLDYNKVIEQVLRGENQVLCQFNFKELLKWLVPISFHLMETLEDLT
                     FAFPENRKDLESARDLIQVIYNKTKDAVEELHKKRSCP"
     gene            1993..3039
                     /locus_tag="VIG33_04400"
     CDS             1993..3039
                     /locus_tag="VIG33_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270104.1"
                     /translation="MRIFFVVCLSFTLIDFKAFASEVPREIALGNSDFTSRTLGVACF
                     GMETVVDGLPCNPAFTAKERRSRFQAQFFFGNNVSYVQDVSRLLDGNGDLSKAESLFN
                     QRYSNEAEANIQVSFLHETWGLRYSPDRLVYYSLIRNSALPVITLYAAQEQTLSGQIA
                     SFTQDHLFWGLQLRAVDRKFLFSEFTLTDALASQGSQYLETKNQRALYVEPGFLWSFD
                     EKPWQPQFGLTVKNAGLVDHKYDELSTNPDWHVAGSVRPPVGLGVFELGLDLHFNSYI
                     AVAKDIPHLGLSYKVGVLQALASYADQEYSLGFLLNYDGWNAGLTYWRRKFENFMGER
                     EQLQTIYLELGFVF"
     gene            complement(3036..3752)
                     /locus_tag="VIG33_04405"
     CDS             complement(3036..3752)
                     /locus_tag="VIG33_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270105.1"
                     /translation="MFHFKSLLSVTLLFFSLKSLAQGDHPRWTLQEWMAQKDKNRLMD
                     LWLSMNSPSPYELMLGISYNSYNLETTGSTTLVPHTSYLGVFSAYAQFVGLTVEYENN
                     AQENLNDLSGLFNLRLLGDTIQGSSLTLHYGMRTRTQGAGATTLPTHYVQQFAQASIQ
                     LYVIKNFGFDGLSRIYSPADDSNLGEIKGNLSEAGLFIDFKNIRVFGTWYKEAQRKEL
                     NTVVTEYTRTGIRSGLKFFF"
     gene            3839..4900
                     /gene="lgt"
                     /locus_tag="VIG33_04410"
     CDS             3839..4900
                     /gene="lgt"
                     /locus_tag="VIG33_04410"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY8270106.1"
                     /translation="MYVHDLNPFAIQFSQSFGIRWYGLSYMVGFICAYLLIRWLTYRQ
                     RAGLSPNMVGDFIIYAAVGTLVGGRLGYVFFYAPDLLLKFKIEFPFWGVFAVNEGGMA
                     SHGGMIGIVIACLLFARRHGVNSLYLLDLVSVSGPIGVFFGRIANFINGELVGRPAPA
                     DFAYAVKFPQDILTWPATDFSKLNSLAGVVDKISISREQWLTMLEKYQLDTLSRAQTY
                     EALQKIVAEIQAGNQGVREALEPLLTARYPSQLFGAVGEGLLLFLFLFFLWRKGRKPG
                     FIAALFIILYAIVRIGDEYFRMPDMQIGYQWLGLTRGQWLSVGMLMIGLSMMFIWMRS
                     NSLNIPGWNRGHSIRLGRK"
     gene            complement(4906..6153)
                     /gene="hisS"
                     /locus_tag="VIG33_04415"
     CDS             complement(4906..6153)
                     /gene="hisS"
                     /locus_tag="VIG33_04415"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY8270107.1"
                     /translation="MSSKFQRVRGTRDLLPEDNIKFRFVESTAHQLSLLYGYGEIETP
                     LFEFSEVFHRTLGETSDVVSKETYTFLDRGGDSITLRPEGTAGVARAFVSEGMAQNLP
                     LKFYYSGPMFRYERPQKGRYRQFKQLGVECLGIDSPLADVECIAMAWDLLGKIGIAGK
                     CHLELNTLGDIESRTEYRKALVDYFSQHQDKLSADSKMRLEKNPLRILDSKDEGDRAL
                     IAQAPALHEHLNATSKKFFDDLIKGITALGVPFEINQKLVRGLDYYCHTVFEFVTSEL
                     GAQGTVLGGGRYDGLIEMMGGVKTPGVGWAAGVDRLSDLVPAEKYQKHQPLLAIIPAD
                     EVGEAACLQVAHKLRLSGLRTELLLSGKMGKRFQKADKIGATHALILGESEVQAHHIA
                     VKDLKSGLQENLSVENLIHKLNK"
     gene            6261..6722
                     /locus_tag="VIG33_04420"
     CDS             6261..6722
                     /locus_tag="VIG33_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HEY8270108.1"
                     /translation="MKKSLIGLGFLVLAVVMGALLYHKFSGGAIKGNGVEVDWRLLGQ
                     MDYVTGKSGSELEALNGREVKIPGFMVPLEDSQKAVTEFLLVPSPQACIHVPAPPPNQ
                     MVYVKMREGAHVTEVAFGPIWVYGTLNLVTKKSMYGEASFELISDGVEPYR"
     gene            6744..7436
                     /locus_tag="VIG33_04425"
     CDS             6744..7436
                     /locus_tag="VIG33_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8270109.1"
                     /translation="MSQLIEVKNIKYRYAKGAQDTLQIAEFSIQKGERVFLYGPSGSG
                     KTTFLEVLAGILKVDQGEVRILGHDLSQMSERSRDHFRAEHLGYIFQSFNLIPYLSVQ
                     ENIELPLHLSHNRRERLKGEDATSVLRHLCTRLGIITLLDKNVTELSVGQQQRVAVAR
                     ALFGRPEFILADEPTSSLDMDHRERFLGLMFELCEQYGTTVLFVSHDRTIQHLFSRSV
                     SLDSVNLIGKGM"
     gene            7438..8703
                     /locus_tag="VIG33_04430"
     CDS             7438..8703
                     /locus_tag="VIG33_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY8270110.1"
                     /translation="MVFLNLALKSLKNRSLASILTVISIALSVILLLSVERAKRAAED
                     GFTQTISQTDLIVGARSGPTQLILYTVFNMGNATHNISYKTYQEIKDYPAIDWTIPYS
                     LGDGHRGFRVVGTTNDFFEHYRFRGEGRVELVEGKTLDGLWDVVIGADVARKLQYHLG
                     EKIVVAHGVTHGEGIQKHDDKPFTVVGIMKPTGTALDQSLYVSLEGVEAMHIDWKGGA
                     APVADTAISPDKIHKEDIKVDQITAFFVRTKSRIETLRLQREINNYKEEPLLAIIPGV
                     TLSELWHGLSYVEQVLKVISWMVVAVGFMAMLIALTTSLNERRREMAILRALGAGTNK
                     ILALLVFESTLLTFTGIVFGTVIAVGVTALLRPWLEIEFGFYLEGSSFGKVEIIYLLF
                     TLVGGILIGLIPAYRAQKMALKDGLSVKI"
     gene            complement(8707..10539)
                     /locus_tag="VIG33_04435"
     CDS             complement(8707..10539)
                     /locus_tag="VIG33_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8270111.1"
                     /translation="MRIPISTKLVVLTVLVLLVATIPIALRTSQIFAKSSMQREEYSN
                     QAFAASRAAEVENILSSIVDKSTTSASILYKSATNPAITSMDEFEMNFIKDKRFVSME
                     ILKLQGTTVELVSRKTKEDFLKTSFNLKPEYISHVRDQQKFPIRSVAQGTVEIRNASF
                     AKGPPMFTIGLPLVKDAQGRITHVALIDVALSVLQKPFSEKSERTLYLIDRNGELITH
                     QDEQKALARLNMAMSPLVKKSMSEQTPRRQMRFQDPDNGTYFIGAYNKTSFGVTVVSQ
                     IPEELVLEPAQDAKRQAFFITGLVLSGALFFIFLFSMTLTSPIEKLAGLIGMVSKGNF
                     DVKATSYVKSHDEVGDLAIAFDHMTDGLKERDKVKNLFSKFHGSSVAEDLINKDIGVG
                     GQSKEVVVFFSDIRGFTAFSEHRSPEQVVAMLNEYFAVMVKVINHHGGVVDKFIGDAI
                     MAIWGAPKGSEEDSRKALRACIEMRKGLADLNEKRIARGEPPLMIGMGLHAGTAISGT
                     IGSDERMEYTVIGNTVNTASRIEASTKAFGADLLVTDDVIAKVGDNFILDYAGAAEVK
                     GRSEAIKMYKVRGYIENGERVEVRTPYSDYEAEGADKVKIKDVA"
     gene            complement(10552..12183)
                     /locus_tag="VIG33_04440"
     CDS             complement(10552..12183)
                     /locus_tag="VIG33_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270112.1"
                     /translation="MSLLRRVLFILTLFTGITGFFSVAWAEAYRREVSFEWEEITDAK
                     SYDIEIRPANKESTDKNMIFRTKSATWSGKLVPGKYTMSLRSKDIRGVPGEWSSPSEF
                     IVNLDPVKLIFPQAQAQIKADNTQEILQKFEWHPVNGASEYHFELSSEDGKTQIVETL
                     KEFNYSVKIPVAKNYTWKVTGLGYAVQSDSTTVANFSVIGKKIEIPKIIAPETEFVRE
                     IKWSRPDSVEKYDLTLKRFNPQTKKWEKVLTEENVQGESFNFAPTHPGGTYSLQVKAK
                     GYMRGDSETSKINFKVVNGNRSPAAEYTALVRKSIDRLTGWYGIASYFITQINYYGET
                     PETGGLTTTNALGGTGRLGAGFFSSDNKWGFLGIVDMSGFVISGANHTYSSLELSGIS
                     RHIIGDLGEARLIAGLYYKEFPQLFGVPSLSGASQVTFFQSSVVGPHLGAEYWHSLSP
                     KLGFQLNTHLYYSLLTIKSPNGNNVEPRISYQLGLLGSYRLNSHFTGLMGLTQRMDQI
                     AYKANKTGAATEGPGDYDSSRITGQYLSFYAEYGF"
     gene            complement(12183..>13095)
                     /locus_tag="VIG33_04445"
     CDS             complement(12183..>13095)
                     /locus_tag="VIG33_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270113.1"
                     /translation="IPINKAPFDMAFKWQKGDETSHMVLEVAKDPALKQKVAVKNFTT
                     EEAFTLPALSEGEYYWRMSSYFEGSEKPVLGKIQKFAVALEPKLEEKVIKPDPVEISW
                     TIPEEKIQFYLEKPMLGLSWNANRPADVASWRLKYYEEGDDPSKVQTLELKGTKTETK
                     VEKPGRYIASLEAFDKNGQIIGTSQARKIAVAPLPLLPPPKLLPLEGNLVAQGDGRTE
                     LQWEKIEGAKEYMLTISNKEGKELKSLKYTNNQTALKNLMPGEYQLTLIAVDQHGRNS
                     EVGITRKLIVPDKSNLKAPALKKIKVN"
BASE COUNT         3315 a         2822 c         3139 g         3819 t
ORIGIN      
        1 cagaagtcca atgaaaacat tcggatagaa taaagacttg ttttaataaa aaccaataga
       61 cctaggtctg gcatattttc tatcatcagt tggaacaaac taccccattc taaaaaacgg
      121 agaggcccat gggggcgttg ataattgacg ttcttagaga taagttcgac acttttcgag
      181 ggtaagtcga aaagtgtcct cttaaattct aaaaccaaag ccattttgaa ttaaaccttc
      241 aaattcaatt aaattaataa caacttacca ggaatggctg caattatcta tcccatagta
      301 ttggtacgaa tatcgcatta tgagacatgc gaaggaccgg acctcagtaa attgggaaat
      361 taatcccact ggatatggaa cggtatcaat atatatgaaa aaattaattt gcttggctct
      421 tatgttatca tcagtgaatt cttttgcaaa tacttacagt ggagtcactc tggaggatgg
      481 gtatccctgt tctttggaaa tgggtttctc tgcgggattt attgccaatt ttcgctatag
      541 aaactttccc gtaggacacg gagaagtgac ttctcaagtg caaagagaat acgaaagatt
      601 tatgagcggt ggcggaggag aaggttcttc caattttacg ttgaaggatc cttctacagg
      661 gaattctatc aatgttactc atagaaattc tgatttgaaa ttagtgtcgg caacccttgc
      721 taaagaagga cgttttatca cttgcgaaat caagtcttgg agaccctaat cctttcactc
      781 cctgagaagg ggccatttga tttccgaatc agtttctctt aatggaacta acaggatctc
      841 gccaagagga gccgacgaga tcctgtgttt ctctgaattt taacggatga tttttttaag
      901 taatataaaa actgcttatc ttatttcaca ctcggtccta attttctaaa tcttactttt
      961 tgaaacttgc taggcatgtg gcctccttta tatggttgat tgtttttggt aaaccatcat
     1021 gcaaagtgta ggtccttaaa gtgaccacac tccattcgat cctgtgattt ttacagatcc
     1081 ctttccttgt ttagctgatt aaacagaatt gagttggccc ctcctttgct taacttcggt
     1141 tatgtcatgg acgactttaa aagctaaatt tggtattggg tctcttgtaa tcttgctgct
     1201 cctttgcgct tgtcagaaag aaagtgtgga ggtgtcaaag gatcccctgg atgagggcta
     1261 caaactcatt gatcagggaa agtattctga agcaatccaa gaactgcagt ccctttcttt
     1321 aagggatttt cgcccctctg tgaaagtggc cctggcctcg gcttacgctg cccgaggaga
     1381 tattcgtgtt gaacactatt ggggatttgt gattggcttt gccactcctt tagtctcctc
     1441 agaaagtagt ttggaaagca gccacattga aagtttacaa aaaattatca aggaaaccca
     1501 ccacagagat gccaatcgtc aagaccttca agccctcgaa gacttgatgg aagccgcgac
     1561 ggtttgggat caatacaaga atcgcttaga tgctattccg tccgtgagtg gagaggctct
     1621 tatggattta aagagcgccg tcgatatcct aggaggagtg caaactcccg gcggacggct
     1681 ctatcgcgcc atcttaaatt tgattttatt caaatcctat atcacggctt ccggcggttt
     1741 ttggttagac tacaataaag tcattgagca ggtattgaga ggagaaaacc aagtcctctg
     1801 tcagttcaat ttcaaagagt tgttaaagtg gctggttccg atttcctttc atttgatgga
     1861 aactttagag gatcttacct tcgcctttcc agaaaatcgt aaagatttag aatcggcccg
     1921 tgatctgatc caagtgattt acaataagac taaagacgcc gtggaagagc ttcataaaaa
     1981 aaggagctgt ccatgaggat ttttttcgtg gtgtgcctgt cattcacttt gattgatttc
     2041 aaagctttcg cttcggaggt ccctcgggaa attgccctgg gcaattcaga ttttacgagt
     2101 cggacattag gtgtagcgtg ttttggaatg gaaaccgtgg ttgatggtct tccctgtaat
     2161 ccagccttca cagcgaagga gcgtcgttct cgatttcaag cgcagttctt ttttggaaat
     2221 aacgtttctt atgttcagga cgtttctcgt ttattggacg gcaatggtga tctttccaag
     2281 gctgaaagtt tgttcaatca aagatatagc aatgaggctg aggccaacat tcaagtcagt
     2341 tttttacatg aaacatgggg tctacgttat tctcccgatc gattggttta ttattccttg
     2401 attcgtaatt ccgcattgcc ggtaataacc ctgtatgcgg ctcaagaaca gactctctcg
     2461 ggacagatcg ctagctttac tcaggaccat ttattttggg gccttcaatt gcgcgcggtg
     2521 gatagaaaat ttttgttcag tgaatttact ttgactgatg ccttggcgtc tcaggggtcc
     2581 cagtatttgg aaaccaaaaa ccaaagggca ctgtacgtcg agcccggctt tctttggtcc
     2641 tttgatgaaa agccttggca accgcaattt ggcctgactg tcaaaaacgc aggtctggtg
     2701 gatcataaat atgacgagct ttcaaccaat ccggattggc atgtggcggg ttcggtgcgg
     2761 cctccggtgg ggttaggagt tttcgaattg ggactggatt tgcattttaa ttcttatatt
     2821 gctgtggcta aagacattcc tcatctggga ctttcctaca aagtgggagt gctgcaggcc
     2881 ttggccagct atgcggatca agaatactct ttaggttttc ttttaaatta tgacggatgg
     2941 aatgcgggac tgacctattg gcgccggaag tttgagaatt ttatgggaga gagagagcaa
     3001 ttgcaaacca tttacctcga gctgggattt gttttttaga agaaaaattt gaggccgctg
     3061 cggatcccgg ttcgggtata ctccgtgacc accgtattga gttccttgcg ttgcgcctct
     3121 ttgtaccaag tgccgaagac tctgatgttt ttgaaatcta tgaagagtcc ggcttcgctt
     3181 aagttgcctt tgatctcacc caggttggaa tcatcagccg gagagtaaat gcgagagagc
     3241 ccatcgaaac caaagttctt aatcacatac agttgaatgc tggcttgtgc aaattgttgg
     3301 acataatggg tcggcaaggt ggttgcgcct gcgccttggg tccgtgtgcg cattccatag
     3361 tgcaaggtca aggaggagcc ttggatggta tcccctaaaa ggcgcaggtt aaaaagtcca
     3421 ctgaggtcat tcaaattttc ctgagcatta ttctcatatt caacggtcaa tcccacaaat
     3481 tgagcgtagg ctgaaaaaac acctaaataa gatgtgtgtg gaacaagagt cgtgcttccc
     3541 gtggtctcca aattgtagct gttgtatgag atgccaagca tcaattcata gggtgagggg
     3601 gaattcatgg agagccataa gtccatcagg cgattcttat ctttttgagc catccactct
     3661 tgcaaggtcc agcgagggtg atcaccttgg gcaaggctct tcagggaaaa gaacagcaaa
     3721 gtaacgctca ggagagattt aaaatgaaac attggaatcc tccattttta attttgccct
     3781 ttagtgtcac ttgggtctaa gattttataa attcgtctaa gattgagact gaggttttat
     3841 gtacgttcat gatttgaatc catttgctat acagttttcc caaagtttcg ggatacgatg
     3901 gtatggtcta tcgtacatgg taggttttat ttgtgcctat ttgctgattc gttggctgac
     3961 ttatcgtcaa cgcgccggcc tttctcccaa tatggtgggt gactttatca tctacgctgc
     4021 cgtggggacc ttagtcggcg gtcgtctggg ttatgttttc ttttatgccc ctgatttact
     4081 tttgaaattc aagatagagt ttcctttttg gggagttttc gcggtgaatg agggcggcat
     4141 ggccagccac ggggggatga tcggcattgt gattgcttgt ttgttgttcg ctcgccgtca
     4201 cggggtgaat tccttatact tattggattt ggtttctgtc tccggaccta tcggcgtatt
     4261 ttttggaagg attgcgaatt ttatcaatgg ggaactggtg gggcgaccgg ctccggcgga
     4321 ttttgcctat gcggtgaagt ttcctcaaga tattttaacc tggccagcca cggatttttc
     4381 taagttgaat tctttggctg gggtcgtgga caaaatcagt atcagtcgtg agcagtggtt
     4441 gacgatgctt gaaaaatatc aactggatac tttatcccgg gctcagactt atgaagctct
     4501 tcaaaaaatc gttgctgaaa ttcaagcggg caatcaggga gtcagagagg ctttagagcc
     4561 cctcttgacg gctcgttatc cgtcccaact ttttggggcg gttggagaag gtttgctcct
     4621 ttttttattt ttatttttcc tgtggagaaa aggtcgcaaa ccaggcttca tagcggcttt
     4681 atttattatc ctttatgcca tcgttcgcat cggcgatgaa tacttccgta tgcctgatat
     4741 gcagattggt tatcagtggc ttggtttaac ccgcggtcaa tggttgagcg tgggaatgtt
     4801 aatgattggt ctttcaatga tgtttatatg gatgcgatcg aactccctca acatccctgg
     4861 ctggaatcgt ggccacagca tccgtttagg tcgaaaatag gaattttatt tattcagctt
     4921 gtggataaga ttctctaccg agagattctc ttgcaagccg gacttcaaat cttttacggc
     4981 aatgtggtga gcttgaactt cgctttcgcc caaaatcaga gcatgagtgg cgccgatctt
     5041 gtcggccttt tgaaaacgct tgcccatctt tccggaaaga aggagttccg tccgaaggcc
     5101 actgagtcgc aatttgtggg ccacttgtag gcacgcggcc tcacccactt catccgctgg
     5161 aataattgcg agaagaggct ggtgcttttg atatttttca gcaggaacca gatcagaaag
     5221 gcgatccacg cccgcagccc agcctactcc aggagttttt actcctccca tcatctctat
     5281 aaggccatca taacgaccac caccgagcac tgttccttgg gcccctagtt cagaagtcac
     5341 aaattcaaaa acagtgtggc aatagtaatc taaaccgcgc acaagttttt gattgatttc
     5401 aaaaggaacc cctaatgccg taattccttt tatcaggtcg tcgaagaatt ttttcgaagt
     5461 tgcattcaag tgttcatgca aagccggagc ctgagcaatc aacgcacggt ctccttcatc
     5521 tttggaatca aggatacgca gaggattttt ttccaggcgc attttgctat ctgcagagag
     5581 cttatcttga tgctggctaa aataatcaac caaggctttg cggtattcgg tgcggctttc
     5641 tatatctcca agagtattga gttcgagatg gcatttgcca gcaatcccaa ttttaccgag
     5701 aagatcccaa gccatcgcga tgcattctac atcagctaaa ggggagtcaa ttcccaaaca
     5761 ctcaactccg agctgtttga attgacgata gcggcctttt tgcggacgtt cgtaacgaaa
     5821 catggggcct gagtaataaa atttaagcgg taaattttgc gccattcctt cagagacaaa
     5881 ggctcgggca acgccggcgg tgccttcagg gcgtaaggtg atgctgtcgc caccgcgatc
     5941 taggaacgtg taagtctctt tgctgacgac atctgaagtt tcaccgaggg tgcgatgaaa
     6001 aacttctgaa aactcaaaga gaggagtttc aatttcacca tagccatata aaagggaaag
     6061 ctgatgggcg gtagactcca cgaaacgaaa tttaatatta tcttctggaa gtaagtcacg
     6121 agtcccgcgg actctttgga atttgctact cattctagca agctaccatc agccttgcac
     6181 aaagggtata ccgtcgcttt cgtggacgca atgcgtgcaa atgggttgcg tttcgcaggg
     6241 gtggctgaga tattcggaaa atgaaaaagt cattgatagg tcttggtttt ttagtgcttg
     6301 ccgtggttat gggcgctttg ctttatcata aattttccgg gggtgcgata aagggaaatg
     6361 gcgtcgaagt ggattggcgt ttactcgggc aaatggatta tgtcactggc aaatcaggct
     6421 cagagctgga agccctcaat ggccgcgaag tgaagatccc cgggttcatg gtccccctcg
     6481 aagacagcca aaaagccgtg acggaatttt tattggtccc cagtcctcaa gcttgcattc
     6541 acgttccggc gcctcccccg aatcaaatgg tttatgtgaa aatgcgcgag ggagcccatg
     6601 tgacggaagt ggcctttgga cccatctggg tttatggaac cctcaatctc gtaaccaaaa
     6661 aatcaatgta cggcgaagcg tccttcgaac ttatcagtga tggggttgag ccatatagat
     6721 aaggctcgtc ctgggggttc tgcatgtctc aacttatcga agtgaaaaat attaaatatc
     6781 gttacgcaaa gggcgctcaa gatactttgc aaattgcgga gttcagcatc cagaaggggg
     6841 aaagagtttt cctctatggt cccagcggtt ctgggaagac gacttttctg gaagttttgg
     6901 cggggatttt aaaagttgac cagggtgagg tgcgaatttt aggtcacgat ctttcgcaaa
     6961 tgtcagagag aagccgagat cactttcgag ctgagcacct gggttatatt tttcaaagct
     7021 ttaacctcat cccttatctc agcgttcagg aaaatattga gctgcctctt cacttgagcc
     7081 acaatcggcg ggagcgtttg aaaggtgaag atgcgacctc cgtgctacga catctgtgca
     7141 cacgtttggg gattattact ttgctggata aaaacgtgac ggaactgagt gtggggcaac
     7201 agcaaagagt cgccgtggcc cgagcgcttt ttggtcgtcc agaatttatc ttagcggatg
     7261 agccgacgtc gtccctggat atggatcatc gggaacgctt tctgggattg atgtttgagc
     7321 tgtgtgaaca atacggaacg accgtgttgt tcgtaagcca tgatcggacc attcaacatc
     7381 ttttctcgcg ttcagtctct ttggattccg tcaaccttat tggtaaggga atgtgaaatg
     7441 gtctttttga atttggctct caaatccctg aagaatcgat ctttggcttc catcctgaca
     7501 gtgatttcca ttgccctgag tgtgatcttg cttctttccg tagagcgggc gaagcgcgcg
     7561 gcggaggatg gttttaccca gaccatcagt caaacggatc tgattgtcgg ggctcgcagt
     7621 ggtcccacgc agttgattct gtacactgtt ttcaatatgg gcaatgcaac ccataatatt
     7681 tcttataaaa cttatcagga aatcaaagat tatcccgcca ttgattggac gattccttat
     7741 tccctcggag atggtcatcg gggctttcgc gtggtgggca ctaccaatga cttttttgag
     7801 cactaccgtt tccgtggaga ggggagagta gaactcgtcg agggcaaaac cctggatggc
     7861 ctttgggatg tggtgatcgg cgccgatgtc gcccgcaaat tacaatatca cttaggtgag
     7921 aaaattgtcg tggcccatgg agtaacccat ggtgagggca ttcaaaagca tgatgacaag
     7981 cccttcactg tagtaggcat tatgaaaccc accggcaccg ctttagatca atctctctat
     8041 gtaagtctgg aaggggtcga ggccatgcat atcgattgga aaggcggagc ggccccagtc
     8101 gcggacaccg cgatcagccc ggataaaatt cataaagaag atatcaaggt ggatcagatc
     8161 acggcttttt ttgtgcgcac taagtccaga attgaaacct tgcgcctgca acgggaaatc
     8221 aataactaca aagaagaacc actgctggca attattccag gcgtgacttt gagcgagctt
     8281 tggcatggcc tttcctatgt tgagcaagtt ttaaaagtga tctcgtggat ggtggtggcg
     8341 gtgggcttta tggctatgtt gatcgcactc actacaagtc tgaatgagcg tcgtcgtgag
     8401 atggctattt tgcgagctct gggggcgggc acgaataaga ttttagcact gctcgttttt
     8461 gaatccacgc tactgacttt tacaggcatt gttttcggca ctgtgattgc ggtcggagtc
     8521 acggccttgc tgaggccttg gttggaaatt gaatttggct tctacttaga gggcagttcg
     8581 ttcggtaagg tggagattat ctacttactc ttcactctgg tcgggggaat cctcatcggc
     8641 cttattccgg cttatcgagc gcagaagatg gctctgaaag atggcctgag tgtgaagata
     8701 taagatctac gcaacatcct taatcttcac tttatccgca ccctcggctt cgtaatctga
     8761 gtaaggggtt ctgacctcga cgcgttcgcc gttttctatg taaccgcgga ctttgtacat
     8821 cttaatcgct tcggagcggc ctttgacttc cgcagctccc gcgtaatcca aaatgaagtt
     8881 gtcgccgact tttgcaatga catcatcagt gactaacaag tctgcgccaa aagctttggt
     8941 cgaagcttca atacgggaag ccgtattgac ggtatttcca atcaccgtgt actccatccg
     9001 ttcatctgag ccaatggttc cggaaattgc tgtgcccgca tgcaagccca ttccaatcat
     9061 caaaggcggc tctccacgag caatgcgttt ttcattgaga tctgcaaggc ctttgcgcat
     9121 ttcaatacaa gctctcaatg ctttgcgtga atcctcttcg gagcctttgg gggcgcccca
     9181 aatcgccatg atcgcgtctc cgataaattt atccacgact ccgccatgat gattgatgac
     9241 tttcaccatc accgcgaaat attcgttgag catcgcgacc acttgctcgg gactgcgatg
     9301 ttctgaaaag gcggtgaaac cacgaatatc gctaaagaac accaccactt ctttggattg
     9361 acctccgaca ccgatatctt tattgatcaa atcctctgct acggaagagc cgtgaaattt
     9421 actgaagaga tttttcactt tatcgcgttc ttttaatccg tcggtcatgt ggtcaaaggc
     9481 aatggctaag tctcccacct catcgtggga tttcacatat gaagttgctt tcacatcaaa
     9541 gtttcctttg gaaaccatgc caatgaggcc cgcgagtttt tcaatgggag aagtcagggt
     9601 catcgagaat aagaagataa agaaaagggc tcccgataaa accaagccag tgataaagaa
     9661 ggcctgacgt ttagcgtctt gagctggttc aagaaccaat tcttcaggaa tctgtgacac
     9721 cacagtgacg ccaaaagaag ttttattgta agcaccaatg aaatacgttc cattgtcagg
     9781 atcttgaaag cgcatctggc ggcggggggt ttgctcgctc atggattttt tcactaaagg
     9841 ggacatagcc atgttgagcc ttgcgagggc cttttgctca tcttgatgag tgatcagttc
     9901 gccattgcga tcaatcaaat aaagcgttcg ttcggacttt tcactaaaag gtttttgtaa
     9961 aaccgaaagt gccacgtcga tcaaggccac atgggtgatg cgaccttgag catcttttac
    10021 caaaggcaga cctatggtaa acataggagg accttttgca aatgaggcat ttcgaatttc
    10081 aaccgtacct tgagccacgg agcgaatcgg aaacttttgc tggtctcgga catggctaat
    10141 atattcaggt tttaaattaa agcttgtttt taagaaatct tctttagttt tacgtgaaac
    10201 gagttctaca gttgttcctt gaagctttag aatctccatc gagacaaaac gcttgtcttt
    10261 tataaaattc atttcaaatt catccatcga tgtgatcgcg ggattcgtgg cgcttttata
    10321 aaggatggaa gcgcttgttg tggatttatc gactatggac gagagaatat tttcaacttc
    10381 agcggctcgg gaggcggcaa aggcttggtt gctgtactct tccctctgca tggaactttt
    10441 cgcaaagatt tgcgaagtgc ggagggctat gggtatggtc gcgaccagta aaaccaaaac
    10501 cgtcaataca actaattttg tagaaattgg tattctcata gttatcctct cctaaaatcc
    10561 atattccgca taaaaactta aatactgtcc agttatccgg ctgctgtcgt aatcacccgg
    10621 tccctcagtg gcggctccgg ttttattagc tttgtaagca atttgatcca tacgctgggt
    10681 cagacccata agacctgtaa aatgactatt gagacgatag cttcccaaga gcccaagctg
    10741 ataagagatg cgtggctcaa cattatttcc attaggagac tttattgtta aaaggctata
    10801 gtacaagtga gtgtttaact gaaagcccaa cttaggcgat aatgaatgcc agtattcagc
    10861 gccgaggtga gggcctacaa cggaagattg aaagaaggtg acttggcttg cgccggaaag
    10921 gctgggaact ccaaacaatt ggggaaattc tttatagtag agaccagcga tcagtcgtgc
    10981 ttcacctaaa tcgccgatga tgtgacggga aatgccgcta agttcaaggg aagagtaagt
    11041 atgattggca ccggaaataa caaatccact catgtcaacg atgccgagaa atccccattt
    11101 attatcggaa ctaaagaagc cggcacccaa gcggcctgtt ccgcccagag cgttcgtagt
    11161 ggtaaggccg ccagtctctg gggtttcgcc ataatagttg atttgagtga taaagtaact
    11221 ggcgatacca taccagccgg tcaaacgatc aatggatttt ctcaccagtg ccgtgtattc
    11281 cgcagcggga gagcgattgc cattgacgac tttaaaattt attttagaag tttccgagtc
    11341 gccgcgcata taacctttgg ctttgacttg gagactatag gttcccccag ggtgagtggg
    11401 agcaaagtta aaactttcac cttggacgtt ttcttcagtc aaaacttttt cccatttttt
    11461 cgtctgagga ttgaaacgtt tgagagtgag atcatatttt tccacactgt ccgggcgtga
    11521 ccatttgatt tcgcggacaa actccgtctc aggtgcgatg atttttggga tttcgatttt
    11581 tttgccgatc accgaaaagt ttgcgaccgt ggtggaatca ctctgtacgg catagccgag
    11641 gccggtgact ttccaagtat aatttttcgc tacggggatt tttaccgagt aattgaattc
    11701 tttgagagtt tccacgattt gagtttttcc atcttccgag gaaagttcaa agtgatattc
    11761 actagcgcca ttgacaggat gccattcgaa tttttgtaaa atctcttggg tattgtcagc
    11821 cttaatctgg gcttgggcct gagggaatat taatttcacc gggtctaagt tgacaataaa
    11881 ttcactgggg gagctccact ctccagggac accccggata tcttttgaac gcaaagacat
    11941 ggtgtatttt ccggggacga gttttccgga ccaggtagca ctcttggttc taaagatcat
    12001 atttttatca gtactttctt tattcgctgg acgaatttcg atgtcataac tttttgcatc
    12061 cgtaatttct tcccactcaa agctgacttc ccggcgatag gcctcagccc aggctactga
    12121 aaaaaatcca gtgatcccgg tgaaaagtgt cagaatgaaa agtacccggc gcaataaact
    12181 catcaattaa ccttaatttt ttttagtgct ggcgctttta aattgctttt gtcgggcacg
    12241 atcaacttac gggttattcc aacctcacta tttcgcccat gttgatccac ggcgatgagt
    12301 gtgagttggt attctcccgg cattaagttt tttaaagcgg tttggttatt ggtgtacttc
    12361 agagatttaa gttctttgcc ttctttgtta gagatggtga gcatatattc tttagcccct
    12421 tcgatttttt cccattgcaa ctccgtgcgg ccatcccctt gggccactaa gtttccttca
    12481 agcggcaaaa gctttggcgg cggcagcaaa ggcaaaggag ccaccgcgat ctttcgagct
    12541 tgggaggtgc cgatgatttg tccattttta tcaaacgctt cgagagaggc gatatagcgc
    12601 ccgggctttt caaccttggt ttcggttttg gtgcctttca attcaagagt ttggacctta
    12661 gaaggatcat cgccctcctc ataatatttc aatctccaag acgcgacatc cgcaggacga
    12721 ttggcattcc aagaaagacc caacatcggt ttctctaaat aaaactgaat tttttcttcg
    12781 ggaatagtcc aagaaatctc aaccggatcc ggctttatca ctttttcttc aagttttggc
    12841 tctaaagcga ccgcgaattt ttgaattttt cccaggactg gtttttcaga accttcaaag
    12901 taagaactca tgcgccaata gtattctcct tcgctgagag ccggcagagt aaaggcctct
    12961 tcagtggtaa agttcttcac agccactttt tgtttcaagg ctgggtcttt tgccacttca
    13021 agaaccatgt gagaagtctc atctcctttt tgccatttaa aagccatgtc gaagggagcc
    13081 ttgttgatgg gaatc
//
LOCUS       DATBTE010000119         3141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_83588, whole genome shotgun sequence.
ACCESSION   DATBTE010000119 DATBTE010000000
VERSION     DATBTE010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83588"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            523..1635
                     /locus_tag="VIG33_04450"
     CDS             523..1635
                     /locus_tag="VIG33_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270114.1"
                     /translation="MKKLLIIAAVTLASGGAFASKARLQALGSSAHLVDVQTMFNNPS
                     DITLMGDMATFEMGTNGPFTYTPPSGNAANLTNHEGGLIRAHGDARWGFYLGHKSPTV
                     ALSRSNAWTGGGTILNEENPIEVFYAQKADLSWGASWFYSNSDKKATKLKQDTMGVRV
                     GARSGAWDASLNVGLSNNAKDESNAASIKEIKGSSAMKLAGGYTMDTLYFYGNYGMSG
                     FKATAASTDTADVSGSQLTLGVVDTIKGEGRDFFYGVSYHMDSYKQKVGTEQKSEATY
                     LPVVIGVEGEATSWMTLRASVTQNVILGTIKDEIGLVTGAALAETNTWNDSTTVAAGA
                     GLKFNKFTLDGTLGAANGGVVNTTNLLANASLTYLF"
     gene            complement(1716..2225)
                     /locus_tag="VIG33_04455"
     CDS             complement(1716..2225)
                     /locus_tag="VIG33_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270115.1"
                     /translation="MKKYFRNSMLAILSMVMAHPAYASDVTIEQIGSFLCEVQLCKNE
                     AVVSNIKASKYNLANLAEKLADLANSAENLPQSGRQVLRLSFHQETPDKEYVRDFRDC
                     NLIVFSPTVMSLSSCKISGTFVPRNSSSVHQLSLGDGEIMHQHPSVGDDEIVRQQEAG
                     WLAGTMSNK"
     gene            complement(2280..2786)
                     /locus_tag="VIG33_04460"
     CDS             complement(2280..2786)
                     /locus_tag="VIG33_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270116.1"
                     /translation="MNANLTILTTVLLTAQAFAGVEPATIRSMITGEMRSQYTQEEAR
                     SMEDLCQLSQNKEIIKWGNEESKLTCNLTSQGHLEITAENHLGKVSIFANPKDMAIVD
                     IVEGDYSYQYTNDIHQQFYLKLTFKKYNASNRPAGSTLKGYYAADLSLRDNSAKIHIG
                     SSLQDYRP"
BASE COUNT          905 a          606 c          666 g          964 t
ORIGIN      
        1 tttttgctca aaattgggtc aggactttgg gcgaggattt tgaaaagaga acgcactgag
       61 gatagtttta gataaaatct gctgcaaatc tcgactttga tctagtgcaa taattctaga
      121 aatgagtatt tgcgtcaaca attaaaaata aagtttgaag cggttgctaa acagagtgaa
      181 aaaaccccta tgaacgaatt ttttaagaaa ttattttgaa caataatttt tctaaaaagc
      241 tctcaggaca aggcattgcg gatgattttc gagagggatt gcgatcaagg ttccgcccat
      301 tgggcaaaaa taattgacga ataaaacttc aaaagaaaga aattaaaagg gctcattttg
      361 cttactaata gtgcattttt ttaagattta aagtttctta accaactttt gtattgatct
      421 cgttatgttt gatttatagg attttttaat aacaaagatt attttttaaa gaaacggttt
      481 taacagactt taaaaattgc taacgattcg aaaggatgca acatgaaaaa gttacttatt
      541 attgcagctg tcacattggc ttcaggagga gcatttgcct ctaaagctcg tcttcaagct
      601 ttgggtagtt ctgctcactt ggtagatgta caaactatgt tcaacaaccc ttcagacatc
      661 actttgatgg gtgacatggc aacgttcgag atgggaacta acggtccgtt cacttacact
      721 ccaccatctg gtaatgctgc caatttaaca aaccatgaag gtggtttgat ccgcgcgcac
      781 ggtgatgcac gttggggctt ctatcttggt cataaatctc cgactgtggc tctttctaga
      841 agcaatgctt ggactggcgg cggtacaatt ctcaatgaag aaaatccaat tgaagtattc
      901 tatgctcaga aagcggatct ttcttggggt gcttcttggt tctattctaa ctcagataaa
      961 aaagccacta aacttaaaca agacacaatg ggcgttcgcg taggggctcg ttctggcgct
     1021 tgggatgctt ccttgaacgt aggtctttct aacaatgcaa aagacgaaag caatgctgct
     1081 tctataaaag agatcaaagg atcttctgct atgaaattag caggtggtta cactatggac
     1141 actctttatt tctatggtaa ctacggtatg tctggtttca aagcgactgc tgctagcact
     1201 gatactgcag acgtaagtgg ctctcaatta actttgggcg tggttgacac tatcaaaggc
     1261 gaaggcagag atttcttcta cggcgtttct taccacatgg attcatacaa acaaaaagtg
     1321 ggcactgagc aaaaatcaga agctacttat ttgccagttg ttatcggtgt tgaaggtgaa
     1381 gcgacttctt ggatgactct tcgtgcttct gtgactcaaa acgtaatttt gggaacaatc
     1441 aaagacgaaa tcggtttggt aactggtgca gctttggctg aaacaaacac ttggaacgat
     1501 tccacaactg ttgcggcggg tgcgggtttg aaattcaaca aattcactct tgacggaact
     1561 cttggcgcag caaatggtgg cgtagttaac actacaaacc ttttggcaaa cgcttctttg
     1621 acttatttgt tctaatttag aactctcagt taaagagaaa ctaaaagaaa actcaaaaaa
     1681 caaaggcccg gtgacaacac tgggcctttg tttttttatt tattgctcat ggttcctgca
     1741 agccatccgg cttcctgctg gcgcacgatt tcgtcatcac ctacgcttgg atgctggtgc
     1801 atgatttcgc catcgcccag gcttagttga tggacgctgc ttgaatttct aggtacaaac
     1861 gttcctgaaa ttttacagct agaaagactc atgacagtcg gtgaaaagac aattaaattg
     1921 cagtctctaa agtctcttac gtattcttta tctggtgttt cttggtgaaa ggataacctc
     1981 agtacctgcc tgccagattg aggtaagttc tcggcactgt tcgcaagatc cgctaacttt
     2041 tcggcaaggt tcgcaagatt gtatttactt gccttaatat tagagaccac agcctcattt
     2101 ttacagagtt gaacctcgca tagaaagcta ccaatttgtt cgatcgtcac atcgctggca
     2161 taagctggat gggccataac catgcttaag atcgctaaca ttgaattcct aaaatacttt
     2221 ttcatataca cctctaaata agttaaagtt gttccaaaga gtttcgtgtt taaattaaat
     2281 cacggtcgat aatcctgcag ggaggagcca atatggattt tggcgctatt gtctctaagg
     2341 gacaaatccg cggcataata acccttaagc gttgaaccgg caggcctatt cgatgcgttg
     2401 tattttttaa aagttaactt caaataaaat tgttgatgaa tatcgttggt gtattgatat
     2461 gaataatctc cctcaacgat atcaacgata gccatatcct taggattcgc aaaaatcgag
     2521 actttaccca agtggttttc agcagtgatt tcaaggtgac cttgagaagt gagattacag
     2581 gttagcttgg actcttcatt gccccattta atgatttcct tattttgaga aagctgacac
     2641 agatcttcca tcgaacgggc ttcttcttga gtgtactgag atctcatttc accagtaatc
     2701 attgacctga tcgtagctgg ctccactccg gcaaaagcct gagcggtcaa caggactgtt
     2761 gttaatatcg taagatttgc attcatagag atacctctct ttactttgac atcttgtcta
     2821 ttatggggag gtgctagata tgtgccatgg ccaaagagag ccttatttca actgaggacg
     2881 gaagagtgcc gaattatgtc acttcgagac gctgttggtc tttttggagg agcgcctctc
     2941 cgttttttag aatggggtag tttgtgccaa ctgatgatag aaaatatgcc agacctaggt
     3001 ctattgattt ttattaaaac aagtctttat tctatccgaa tgttttcatt ggacttctgg
     3061 ttgtactcaa acagaagtaa agtcgcgcta cgagtttcaa aatgaataat aagctccttc
     3121 gcgacttatg atagccgtat c
//
LOCUS       DATBTE010000120         6423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1312758, whole genome shotgun sequence.
ACCESSION   DATBTE010000120 DATBTE010000000
VERSION     DATBTE010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312758"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1734)
                     /locus_tag="VIG33_04465"
     CDS             complement(16..1734)
                     /locus_tag="VIG33_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270117.1"
                     /translation="MKKHIKDIAVILGLFFVTLWVCKDAFFYGVEMDEVLRLNPLFPL
                     IGKTVPYPDQGLYYIDLFGVRIPLVFKLYVSSAIMLPYYPLTFFADPFVGLRVMSGFY
                     LFLAGVTVYLVAKKYSRIWAFLLALSILMSPLLGAHLLVRQAQTYHIICYALSFYFIR
                     KFLENNNQKNRNFIYVFFSSFFIFLAMNLEFYAAWSVVSLALVVPLLCWPQVKAYFAE
                     GKAKLIFLLGAVGCINFIWYNLADGLPVLRTVYSYVFKIEEYNKEPIDGRNIVSLSED
                     FGSKWSSLRHYWGPSFRVFLLLFAIMAVITLICLFRDIKKKQKTHFLFYVPFFVTLFV
                     CVFIFLSPKANRSGHFAHVHPFLNLCFFLLPVLVFNLKETKKWFFYFCSFLPIAFLGL
                     NIVAVNASLKNALVRYSTGLWSSSFNTLNHYLEETPEVYNKMVVLNWGFSEQLFFLNK
                     GQKLPVEFVFPVMMRASQSEMINDYQVFFESVGSGEFFKYRHMDAKKEELVFLKFIKG
                     SAAAINRSNEALEVFLKEKDGQYEVLKEFADSDGTPLFHIIRLKNPEVFYNKVKGGAN
                     PSVVSK"
     gene            complement(1731..2504)
                     /locus_tag="VIG33_04470"
     CDS             complement(1731..2504)
                     /locus_tag="VIG33_04470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8270118.1"
                     /translation="MLSETEQKQKDYYNSIAVTYDQHYSNEYALTYRNKQFDSFLEGQ
                     DLQGKLVLDACCGGGQNTSYFLSRGAKVIGMDISEKQCEHFAKHYPQAEVVCGSVLVT
                     SFPDAHFDFIVTESFHHLHPYTAKGLKEYMRILKPGGKLFLWEPHSGSILDFARKIWY
                     RLDSNYFEENESSINIEEFKKYLKGTAQVKKIRYGGDLAFLAVMSSMHFRIPPKWVGL
                     YAKLFCFLEKLWSPLQSRFTSCWVMAMFEKNMNNKKDLV"
     gene            complement(2464..3783)
                     /locus_tag="VIG33_04475"
     CDS             complement(2464..3783)
                     /locus_tag="VIG33_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY8270119.1"
                     /translation="MSQGAPNPRYRIYSKLWYYTRFFSDLMFGKFHKGNDVELLEDKL
                     KSRFGVRNAVCTPMARVGIYLAVKNLIKPGQKVIMSPYTIADVVNMVIAAKGVPLFAD
                     IERATCNIRADEVARLITQPNVGAVLITHLHGLAASVAEIQQICEKHNIPMLEDCAQA
                     FGTVVGGRPVGCFGKAGIYSLGMYKNINSWYGGFVITNDDSLAQVLRKEISSWPYQNT
                     FFIFKRMFKSLKADVLTIPILFKSFTFWIFKHGFINDIEFINKFVRTELDTRRHDEFP
                     ERYKGKLTPYQARLALSQLDLINPDTEIRIKYADKYRLALSGYSDLILPPTVDKFVHM
                     YTYFPIQYKERDTLIKWMMKFDRDLAAQHLKNCADLESFKEFYRDCPNARATAEQVIL
                     LPTYPRYGYEDVEKNIEVLKAFFKPVTAAQKEYSNVVRNRTEAERLL"
     gene            complement(3809..5302)
                     /locus_tag="VIG33_04480"
     CDS             complement(3809..5302)
                     /locus_tag="VIG33_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270120.1"
                     /translation="MKVGIVGTGPAAFALVEALTAYLKVEVVLFEFGQELLVEKPCTS
                     VEVHEMKRFYSELYGKVKKTVGLKFPPQKSYFHHPLPSHTLEGKKRFPRSQAFGGLSN
                     FWGATMLPMTAEEASEAGFSPETLVESYQAVAKLVGVSGRSDGLTPAYGCEYSTIPAV
                     EQLKGVQGFSEVMNRKSNVAGYHFFSGVNRVAVNTKPDHKNSCVYCGECLLGCFRESI
                     FNSKNRLSEMMTQGVLSLVNERVVKVHRSENGATISTGTSDYHFDKVFLCAGAVGTAE
                     ILLRSFAPQEEVILSDNSICQVPILNFSSHSDELKSKYLSLTQAITVIKEGEGSSPQN
                     IAQMQIYPNFDYLWRSAVPEFLWPLLSPLVEWSRDRLLWGRLYTHGSYGWKYQLSLNS
                     EQELKINLMQHPVKEVSKKFIKALACQLKGSGYRVVDFKPMFAKTSSHLAIKKPASLQ
                     IDSLGRIANNIFVCDGAYFEESPATSLTFSIMANSYRIGKAVALGEC"
     gene            complement(5303..5845)
                     /locus_tag="VIG33_04485"
     CDS             complement(5303..5845)
                     /locus_tag="VIG33_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8270121.1"
                     /translation="MNAKVKAKELYSNWDLLGVILSNWRTDLVIKHLPEGTFLDLACG
                     DNRLVKKLGRGIGIDISNYGGSADMVLPDFSKLPFEKNSINAVTVLAAFNYFDQPVSV
                     LKEIRRVLSDDGCLIITQLDHRISKAWHQLRDRGLVRTAYSDQQLQEMFTAADLRIDV
                     KERFMLGLNKVFVVRKKLKV"
     gene            complement(5829..>6423)
                     /locus_tag="VIG33_04490"
     CDS             complement(5829..>6423)
                     /locus_tag="VIG33_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270122.1"
                     /translation="GAVSKTWVSMTLVLEFFFAFLASFLLSLPCLFKQFSLLKEWNSS
                     VLVVGVGCFGVLVVIAGIFLSKKFKSAVADFFRLLLSEKRKLLKAFALMFIAFFIMSL
                     SFAVLGAGMIRAELLWPFLFAFPLAWILGYLTPGSPAGMGTRELALVMLLSVYASQTE
                     VSQVAILMRVVSLSSDVILYLGGYFLSKRVLSYECES"
BASE COUNT         2081 a         1538 c         1163 g         1641 t
ORIGIN      
        1 atgaagttct ctcgtctatt tgctcacaac gcttggatta gcgccgccct ttactttatt
       61 gtaaaacacc tcaggatttt tcaacctgat aatgtgaaat aagggagtcc catcgctatc
      121 agcaaattct tttagaactt cgtactgccc gtctttttct tttaagaaaa cctctaaggc
      181 ctcatttgat ctattaatgg ctgctgctga ccccttgata aactttaaaa aaaccagctc
      241 ttcttttttc gcatccatat gcctgtattt aaaaaactcc ccactcccca cagactcgaa
      301 aaagacttga taatcattga tcatttcgct ttgactagct cgcatcatca caggaaatac
      361 gaattctacc gggagttttt gacctttatt taagaaaaac aattgttcag agaagcccca
      421 gttcaacacc accatcttat tataaacctc tggggtttcc tctaaataat gattgagtgt
      481 attgaacgac gaagaccaca atcctgtgga atatctaacc aatgcgtttt ttaaagaagc
      541 attaacagca acaatgttta ggccaagaaa ggctatgggc aaaaaggaac aaaaatagaa
      601 aaaccacttt ttagtctctt ttaaattaaa taccaaaaca ggcaaaagaa aaaagcaaag
      661 attcaagaaa ggatgaacgt gggcaaagtg cccgcttcga ttggccttgg gactcaaaaa
      721 tataaataca cagacaaaaa gagttacgaa gaaaggaacg taaaataaaa aatgggtctt
      781 ctgctttttt ttgatatcac gaaaaaggca gatcaaagtg atcacggcca tgattgcaaa
      841 caaaagcagg aataccctaa atgacggtcc ccagtagtgc cgcagacttg accatttgct
      901 accgaagtct tcactgaggg atacaatatt acggccatca atcggctctt tgttgtattc
      961 ttctatctta aatacgtaac tataaaccgt tcttaaaacc ggaagtccat ccgcgagatt
     1021 gtaccaaata aaattgatac accccacagc ccccaataaa aatatcaact tggctttgcc
     1081 ttccgcaaag taagctttta cttgtggcca gcaaagcaaa ggaacaacta aagccaatga
     1141 aacaacggac caggcagcgt aaaactcaag attcattgct agaaaaataa aaaaactaga
     1201 aaagaataca taaataaaat tacggttttt ttgattgtta ttctcaagaa atttacggat
     1261 gaaatagaaa ctcaatgcat aacaaataat atggtaagtc tgagcttgtc gaaccaaaag
     1321 atgcgcacct aacaagggcg acatgaggat actcaaagcc aatagaaaag cccaaatacg
     1381 agagtatttc ttagccacca agtaaaccgt gactccggct aaaaacaaat aaaagccact
     1441 catcacgcgt aaaccaacaa aagggtcagc aaaaaatgtt aaggggtaat aagggagcat
     1501 gatggcgcta gaaacatata acttaaaaac gaggggaatt cggacgccga acaagtctat
     1561 atagtaaaga ccctgatcag gatagggcac cgtcttgcca atcagtggaa aaagaggatt
     1621 cagtcttaaa acttcatcca tctccactcc gtagaagaaa gcatctttgc acacccacag
     1681 agtcacaaaa aacaatccca atatgacagc aatgtcttta atatgttttt tcaaaccaaa
     1741 tccttcttgt tattcatatt tttctcaaac atcgccataa cccaacagga ggtaaaacgg
     1801 ctttgcaaag ggctccaaag cttttcaaga aaacaaaaga gcttcgcgta aagacccacc
     1861 cactttgggg gtatacgaaa gtgcatgctg ctcataaccg ctaagaaggc caaatcacca
     1921 ccatatcgga tcttcttaac ttgagccgtg ccctttaaat attttttaaa ctcttcgata
     1981 tttattgaag actcattttc ttcaaaataa ttactatcca aacgatacca aatttttcta
     2041 gcgaaatcca aaatagatcc agaatgaggt tcccacaaaa acagtttccc cccgggtttc
     2101 aaaattctca tatattcttt caaacccttt gccgtatagg gatgaagatg atgaaaagat
     2161 tccgtcacaa tgaagtcaaa atgagcatca ggaaaagagg tgaccaagac gctgccacag
     2221 acaacctccg cctgcggata gtgctttgca aagtgctcac actgtttttc agaaatatcc
     2281 atccctataa ctttcgctcc gcgcgaaaga aaataactcg tgttttgacc cccaccacaa
     2341 caagcatcta agaccaattt cccttgaaga tcctgacctt ctaaaaagga gtcaaattgc
     2401 ttgttccgat atgtaagggc atactcattg ctataatgtt gatcataagt gacggcaatg
     2461 gagttataat aatctttctg cttctgttct gtttctgaca acatttgagt actctttctg
     2521 tgctgcagtg accggcttaa aaaatgcttt taacacttca atattttttt ctacgtcctc
     2581 gtatccatat cttggatacg taggtaaaag aatcacttgt tccgccgtcg ctctggcgtt
     2641 cgggcaatcc ctatagaatt ctttaaaact ctccaggtct gcgcaattct taagatgttg
     2701 agccgccaaa tcacgatcaa acttcatcat ccacttaatc aaagtgtcac gttctttgta
     2761 ttgaatggga aagtaggtat acatatgtac aaacttatcg acggtgggcg gcaagatcaa
     2821 atcagaataa cccgacagag ccagtcggta cttgtccgca tatttaatac gaatttcggt
     2881 atctggattg atgagatcga gttgcgataa cgccaaacgc gcctgataag gtgtcaactt
     2941 gcctttgtaa cgctctggaa attcatcatg gcgacgcgta tccaattcag tcctcacaaa
     3001 cttattaata aactcaatat cattaatgaa tccatgctta aaaatccaga atgtgaagga
     3061 cttaaaaaga atgggaatgg tcaaaacatc agccttaagt gacttgaaca ttcttttaaa
     3121 gataaagaag gtattttgat agggccaaga cgaaatctct ttgcgcagca cctgagctag
     3181 tgaatcatca ttagtgataa caaatccacc gtaccacgag ttaatatttt tatacattcc
     3241 cagactgtaa attcccgctt tgccgaagca ccccacggga cgcccaccta caacagtacc
     3301 aaaagcctgg gcgcagtcct caagcattgg aatattgtgt ttttcacaaa tctgctgaat
     3361 ttccgcgaca gaagctgcca atccatgaag atgggtaata agaacggccc caacattggg
     3421 ctgcgtaata agccttgcca cctcatcggc tctaatgttg caagtagctc tctcaatatc
     3481 agcaaaaagg ggcacccctt tcgccgcgat caccatattc acgacatctg caatggtgta
     3541 gggagacatg atcacttttt gccccggctt aatgagattt ttaacagcca gatagattcc
     3601 tacacgagcc atgggggtgc atacggcatt gcgcactcca aaacgggact tcagcttgtc
     3661 ctctaataac tctacatcat ttcccttatg gaactttcca aacataagat cagaaaagaa
     3721 gcgggtgtaa taccaaagct ttgaatatat tcggtaacgt ggattggggg ctccttgact
     3781 cattatctac tccttaattt aataaaaact agcattcccc taaggccaca gcctttccga
     3841 ttctatagct attcgccata atcgaaaaag tcagtgaggt cgccggagat tcttcaaaat
     3901 atgcaccatc acaaacgaaa atattgtttg cgattcttcc aagactgtca atctgaaggc
     3961 tagcaggttt cttaattgcc aagtgagaac tcgtcttggc aaacattggc ttgaaatcca
     4021 ccactcgata accgcttcct ttaagctgac aggcgagagc ctttataaat tttttagaaa
     4081 cttcctttac gggatgctgc attaaattta ttttcaactc ttgctcagag ttaagactta
     4141 attggtactt ccaaccataa ctcccgtggg tataaagccg cccccacaac aaacgatccc
     4201 gactccactc gaccaagggg gacaacaagg gccataaaaa ctcaggaacc gcactccgcc
     4261 acagataatc aaagtttgga tagatctgca tttgagcaat attctgcgga gaactgccct
     4321 ctccttcttt gatgaccgtg atcgcttggg ttaaagaaag atatttcgac ttcaattcat
     4381 cggaatgaga agaaaaattc agaatgggca cctggcaaat ggagttgtcc gataagataa
     4441 cttcctcttg cggagcaaaa ctccgcaata aaatttcagc ggtcccaacc gccccggcac
     4501 aaagaaacac tttgtcaaag tgatagtcag aggtcccagt tgaaatggtg gccccatttt
     4561 cactcctatg aactttcacc acccgctcat tgaccaagga gagcactcct tgagtcatca
     4621 tctcacttaa tctgttttta ctattgaaaa tggattctct aaaacatcct agcaaacact
     4681 ccccgcagta gacgcaagaa ttcttgtgat cgggtttggt attgaccgcc acccgattca
     4741 ctcccgaaaa aaagtgatat cctgcaacat ttgatttacg attcatcact tcagaaaatc
     4801 cctgcacccc ttttaactgc tccacggcag ggattgttga gtactcacat ccataggcag
     4861 gagtgagtcc atcgcttctt ccagaaaccc cgaccagctt tgcaacagcc tggtaagact
     4921 cgacgagagt ttcagggcta aaaccagctt cgcttgcttc ttccgccgtc atgggaagca
     4981 tggttgcgcc ccaaaaatta gacaatccac caaaggcttg actccgagga aacctcttct
     5041 tcccctctag ggtatgggag ggcaagggat gatggaaata agatttttgc ggtggaaact
     5101 taagccccac agtcttttta acttttccat acaattctga ataaaatctt ttcatctcgt
     5161 gcacttccac cgaagtgcac ggcttctcca cgagaagctc ttgaccaaac tcgaataaga
     5221 caacttccac ttttaaataa gcagttaatg cctccactaa ggcaaaagct gcgggtcccg
     5281 taccgacaat tccaactttc acttagacct tcaacttttt ccgaaccacg aagactttat
     5341 tgagccccaa cataaaacgc tctttcacgt caatccttag gtcagcggca gtaaacattt
     5401 cttgcagctg ttgatccgaa tacgccgtac gtaccaaccc acgatctcga agttgatgcc
     5461 aagctttaga aatccgatga tccaattgcg taatgattaa gcacccatca tctgacaaaa
     5521 cccggcgaat ctccttgaga acagagactg gttggtcaaa atagttaaat gccgccagca
     5581 ctgtcaccgc atttatagaa ttcttctcaa agggaagctt tgagaagtct ggcaatacca
     5641 tatccgcgct ccccccatag ttacttatat caatgccaat gccgcgaccc aacttcttga
     5701 ctagcctgtt atccccacat gctaaatcca aaaaagtccc ctcaggcagg tgtttaatca
     5761 ctagatccgt acgccagtta cttaaaatca cacccaagag atcccaatta ctatataact
     5821 ctttagcctt aactttcgca ttcataactc agaaccctct ttgaaagaaa atatccacct
     5881 aaatataaga taacatctga agataaagat actacgcgca tcagaatggc cacttgcgac
     5941 acttctgtct gtgaagcata aacacttaag agcataacca gcgcgagctc ccgggtgccc
     6001 attcccgccg gcgaacccgg agttaaatag ccgagaatcc aggccaaagg aaaagcaaaa
     6061 agaaagggcc acagcaattc cgcccggatc attcccgcac ccagcacggc aaaggaaaga
     6121 gacataataa aaaatgcaat aaacattaaa gcaaaggcct ttagaagctt tcgtttttct
     6181 gaaagaagca ggcgaaagaa atcagcgact gcactcttaa actttttact caaaaagata
     6241 cccgcaatca caacgagaac tccgaagcaa cctactccca caactaaaac tgaagagttc
     6301 cattctttga gtaaagaaaa ctgcttaaat aagcacggca aactcaacaa aaaactggcc
     6361 agaaaggcaa agaaaaattc tagcaccaag gtcattgaca cccaggtctt tgaaaccgcg
     6421 ccc
//
LOCUS       DATBTE010000121        24307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_835063, whole genome shotgun sequence.
ACCESSION   DATBTE010000121 DATBTE010000000
VERSION     DATBTE010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 24307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24307
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835063"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VIG33_04495"
     CDS             <1..204
                     /locus_tag="VIG33_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEY8270123.1"
                     /translation="FEKAQHCCEYTHPLTGKRCGSEFQLQIDHVRPLACGGSDEIKNL
                     RALRGLCNRREAQRWGLQRPTEN"
     gene            complement(225..1454)
                     /locus_tag="VIG33_04500"
     CDS             complement(225..1454)
                     /locus_tag="VIG33_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY8270124.1"
                     /translation="MFMEISQASHAAKEETLKQCRDSYKAFLQRSEIGFFKLPERREL
                     WLQAEKYEEKLSQFKNLIIVGLGGSSLGTRVIQEVAQVKNIFFVDNVDAIEFESQIAN
                     LLKDMASFKSTCWAFVSKSGTTIESLCALELLQQIYQEKDLQLADYSLVISETKESSL
                     TRWARAHKVPEFEIPQDVGGRFSVLSAVGLVPAAFAGMSLEKIRQGALQALQDQEMVV
                     TLMAQFVQSFERGEWISLLWSYNARMKNFGLWFQQLWAESLGKATKRDGSRAPRVSTP
                     LCAIGASDQHSILQQVMEGAKDKFVVFQRFGDSEGGRMKIQKAQFPETQCLQGRSMGE
                     LLGVEAASTQEALNQKGVSTLTLKFKALNEESLGYQFMLWELVVAGLGEYLGINAFDQ
                     PGVELGKILAKRKLGSS"
     gene            complement(1540..3705)
                     /locus_tag="VIG33_04505"
     CDS             complement(1540..3705)
                     /locus_tag="VIG33_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY8270125.1"
                     /translation="MSMQESIRIVSQGDIAIVELDLVGEKVNKFSTPVMARMKDVITE
                     LGKSSYKAVIFVSKKSKIFIAGADIEEIKNMTTREQYEAAVRGGQEVMNMVEDLPMPT
                     IAAVNGACMGGGCEFILACDYRMATEDSSTKIGLPEIQLGILPGFGGCVRMPKLVGLQ
                     ASLDIILAGKSVNAKKASKIGLVDRVVHPAILMDEATKWAKEIIGKGTSKAVKKYQPQ
                     GAMNKVLESFFGRGIVFKKAKEGVLKATGGHYPAPLKALEVIQKTYGWSNTQEGREQA
                     LAVERTGFCDLATTEISKNLIHVFYLTEMVKRSSGVPGKNVKGREVKSMGILGAGTMG
                     GGIAYVAADKGIEVRMKDLNPEALGKGLKHASDLWMKLLKRKSIDKYQYRQKIDKVTV
                     ATDYAGFMNLDVVVEAIVEDMAIKQKVIAECASKMRADAILVTNTSSLSVTEMSKGHP
                     RPEYFAGMHFFNPVDKMPLVEVIRGERSSDETVATIFELSKKMGKMPVVVKDGPGFLV
                     NRLLLPYMAEAAFLLQDGMDIKTVDMAFVKEFGMPMGPFALMDEVGLDVCIKVLKIFK
                     KSFGDRVEMAPCMDILEKSGRLGKKNGKGFYTYSETGKRLEVDETIYAALSLKPATSP
                     LTSKECIERGIFAMVNECSRALVEDKIVETPHEVDLAMIMGTGFPPFRGGLLKYADSV
                     GLTYIETQLAEYAVQRNAARLRPANPLKDLAKTNKKFYT"
     gene            complement(3711..5069)
                     /locus_tag="VIG33_04510"
     CDS             complement(3711..5069)
                     /locus_tag="VIG33_04510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY8270126.1"
                     /translation="MSTTANRSKNPRDVVIVEGVRTPFAKAGTKLKSLHPAELGKVAL
                     KELIARANLDVNVVDEVIIGNTGNPVDAVNISRVVALNAGIPMKTSAYTVHRNCASAL
                     ESISNGYEKIKSGTMDVVIAGGVENMSQMPTLLPTKIQALFDKLFAAKGPQQALPLLW
                     KLFKADVNQIKALLQGNTRNEYFPVISVLQGLTDPFVGINMGQTAEILAKEWGLSRQT
                     QDEFALRSHKLASANKAKLKEELAPVYIAPDYKEVFNEDNGPRESQTLEALAKLKPFF
                     DKQTGTITAGNSCPITDGAAMALLMSREKAESLGHKPVARIVSYGFAGLEPERMGLGP
                     VYSTPVALKRAKLSLKDIGLIELNEAFAAQVLSCVKAMDSDKFAQEKLGLSQKVGEIN
                     LDQVNVNGGAIALGHPVGATGTRLVVTLMKEMQRRDVQFGLATLCIGGGQGGSMILER
                     EG"
     gene            5365..7707
                     /locus_tag="VIG33_04515"
     CDS             5365..7707
                     /locus_tag="VIG33_04515"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEY8270127.1"
                     /translation="MSEKLEGHQQGNQILSPLEQEALDYHAKGKPGKIEITSSKPCNT
                     EKALSLAYSPGVAVPCKVIAKDPAKVYDYTSKGNLVAVISNGTAVLGLGNIGPLASKP
                     VMEGKGILFKQFAGIDVFDIEMKTNTTAELIAAIKALEPTFGGINLEDIKAPECFEVE
                     ETLKKIMNIPVFHDDQHGTAIVSGAALLNACYITKRKLEDIRLVVNGAGAASIACSNI
                     FISLGVRRENLIMCDTQGTIYKGRTAGMNKYKEAFAVETEARNLHEALQGADVFIGLS
                     VAGALTAEMLQGMAKDPIIFAMANPDPEIEPAKARAIRPDAIVATGRSDFPNQVNNVL
                     GFPSIFRGALDTRSTQINEEMKLAAVHALAMLAREDVPDRVSDSYGGQSFKFGREYLI
                     PKPFDPRVLLKVAPAVAKAAMTSGVATKQITDWEDYSDRLEAIQGPSKVFIRSAIHRV
                     RQNIEAHGGELPKIIFPEGHSKKILKALRNLVEERICQPILLGYPERIHEKMHNMDLT
                     DTLKHIQVIQPSNHPKFKAYAQELYVRRNRKGVNYREAERLMSDPNYFASMMVQMGDA
                     DGMVTGATQHYADSVRPIFHTIGISKEAVPAGLNLVLLEDRFLLLADTTVNINPTAEQ
                     CAIIALEAARIAEYFGVSPRIAMLSYSNFTGGDGTPAKMKRAAELVKKRRPDIPVDGD
                     MQADTAVNDEIIKRIFPFCDLKDGGANILIFPNLESSNICYKLLQQIGKAEVIGPFLM
                     GVRRSANVLQRTTTVDAIINSVVLTTLEAQYIKDAIKKKK"
     gene            7895..9163
                     /gene="hflX"
                     /locus_tag="VIG33_04520"
     CDS             7895..9163
                     /gene="hflX"
                     /locus_tag="VIG33_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY8270128.1"
                     /translation="MTQNQVHIKTSDRAIVVGVGLKTEPISEIKENLLELEELVSAAG
                     GEIVGSVIQMLPAWNPATLIGTGKVDEIRNMVSESQASVVVIDHQLTGVQSRNLAQAM
                     GTRVIDRNQLILDIFAQRAQTFEGKLQVELAQLLDQMPRMVGAWMESLSRQGGGIGTR
                     GPGETALESDRRRIREKVAIIRKKLDSVRKNRAQHRSSRRRNEIPSFALIGYTNSGKS
                     SILNRLSGSQVLAKNQVFATLDPTTRKIFLPEGPPAVVTDTVGFIRKLPTQLIEAFKA
                     TLEESAEADVLLHVIDLSSPNMLRQIEVVESLIQEFNWQDKKIIHVFNKTDAAPIERQ
                     FQVKLYPRVFVSALTGQGMEQLKKLMVESVQEMQTDVQLYFPRSQEFKIYDLDRETKI
                     LRKETATEGTVCYTQLTPALISRWKDYLVK"
     gene            9441..10448
                     /locus_tag="VIG33_04525"
     CDS             9441..10448
                     /locus_tag="VIG33_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270129.1"
                     /translation="MRLTYLTAKSESVTPIKNSSHPPCLETDTNKKQLPYLQEIAKVA
                     TQKINSLFAPEPAAKINRECIAAAMKRNPGDTGILCTTPKSISRKNKTNQCINDNTVD
                     YIHFAVNSAIQCMSSDDPIDSRVLLKKFNNETAFHPSIASKGGVGLGQMTSIAVNEIS
                     QGQGRYILENIAASRKTECKGFRDIAAKDLEKNPRINSKNFCPWVSPGDGLARNLIYS
                     IGYYLTLRDQYIIPAVERRSSSLSSSKDLINDLTNISYGPEGLKQVEWILQKYRVNNK
                     TSTQTLQSQVNKNSKYLEDTKNKMHELTCIRKDLNPAGKECENYKLTIDELKANTCIS
                     N"
     gene            10433..10861
                     /locus_tag="VIG33_04530"
     CDS             10433..10861
                     /locus_tag="VIG33_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270130.1"
                     /translation="MHFQLIFLLFLFPAFSYATLQTTAEFMEQSHKTRETLSKDASFK
                     KIADELQTFEKSLNTAIQEYEKDSPLEGGDEEEKVFKLSFGFKNIFNLAKQKSASKEA
                     CEKAKHQIEFEDKSGLDEDAKLSEAAEEALKWLELLCKTF"
     gene            complement(10858..11271)
                     /locus_tag="VIG33_04535"
     CDS             complement(10858..11271)
                     /locus_tag="VIG33_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270131.1"
                     /translation="MKYLIFFSALVLPFCSFAETRYSVALGSDRHEILWLRQRKIQNK
                     EVYLIGYQSPQKRQLEKVMPHQAFSVLVKEIQSFSKSITQEQFRHLNPFCQEKVFIEN
                     NNKAKDLCLDFMDQKEKMKLVQWVAKQSNVVIGID"
     gene            complement(11268..11645)
                     /locus_tag="VIG33_04540"
     CDS             complement(11268..11645)
                     /locus_tag="VIG33_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270132.1"
                     /translation="MADVVGSVIAEKYIIENPFKNEEEKVAAIRFIDGCSLNNSVVKR
                     PESLAPKTLETLFSSSSFSDSHPENSDRVKKIMLNLPGMAKIYDCQRKAPACFDHLSF
                     STRMGSAGASRNDSELRMQEGEK"
     gene            complement(11633..12187)
                     /locus_tag="VIG33_04545"
     CDS             complement(11633..12187)
                     /locus_tag="VIG33_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270133.1"
                     /translation="MSLVKLLGIFLLMSFAAEKNDSKARSKGKSVCDSNTVTRNCDFF
                     RERASQRVITLADGSTFYNPLFVDPQKPQLPNLDNPPGGGIGGNGYIGGMDYDKLIYN
                     QSEIIDALESTSFSTRFKMMYAGQIAGLALVPDQAVEIPWPPTDKDAKKQSIKPAEIE
                     AYIKKNLSAEDWLRHHRWRKQWRM"
     gene            complement(12212..12685)
                     /locus_tag="VIG33_04550"
     CDS             complement(12212..12685)
                     /locus_tag="VIG33_04550"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY8270134.1"
                     /translation="MGNAKPVSASQVIMTQLVLPSHINAMGSIFGGTVMSWIDIAAAI
                     AAQRHSNKEVVTASMDSLSFIAPVYKGWVVNLKASVNFTSRTSMEVGVRVDAENPRTG
                     EVFHTASAYTTFVALGSNGKPAEVPALVLETADEKRRFEAARKRREARLKSRQNS"
     gene            complement(12730..13140)
                     /locus_tag="VIG33_04555"
     CDS             complement(12730..13140)
                     /locus_tag="VIG33_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8270135.1"
                     /translation="MLRDVLIQKGLSNREAEVAELVSKGLSNKEVANQLFVTEKTVKF
                     HLTNIYKKMNVKSRAQLIVWCLPHLGFVENETRTEMNINQGVAAAAYNNQATQMIPAG
                     HSTVAGVATTLPGAQNGIGRTGTGNSNGDIGAGI"
     gene            13387..13812
                     /locus_tag="VIG33_04560"
     CDS             13387..13812
                     /locus_tag="VIG33_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8270136.1"
                     /translation="MFPNDIKFLVVDDFATMRKIIKKVLNELGYTNIEESDDGKNALP
                     ILQAAVSNGQPFGFIISDWNMPGMTGIDLLKACKADANLKNLPFMLVTAESEQKHILE
                     AAKAGVSDYVVKPFNAATLKAKMERIYQRHSTPPAAKAS"
     gene            13870..14376
                     /locus_tag="VIG33_04565"
     CDS             13870..14376
                     /locus_tag="VIG33_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HEY8270137.1"
                     /translation="MSAAPKIVPEVNPLFDKKLLQAFVDGALKTLATIAQTEAKVGKP
                     HIETRLSEKCEIAGMVGMVAPPLKGTLLISFTKESIFQIMENMLGEKHTEISNEISDA
                     VGELTNMIYGTAKTALNQMGYNFEMAIPTVIRGEFTLSKSTKGATLVIPFELKNNSAF
                     HIAITVQT"
     gene            14386..15765
                     /locus_tag="VIG33_04570"
     CDS             14386..15765
                     /locus_tag="VIG33_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY8270138.1"
                     /translation="MPKKPIDSQTDVLIASQRENFWQDVQIILKGFYPYKLHFVRSSE
                     SIINPTEEITPLLTLVDGFGGTPQTCEWVQSVKMAFPNCPLIVLYGPEDSLDFSLVKK
                     NGANFIMHTNYDREFISDMILNLAPVEIQGTDLPTSALLPIDMKDIDPEEPINFDIFI
                     HLPTNHKSFRVRKNGGKIDPRLLEKSSETHQRLYIKKTQIKPFFEYARTVLTMRNLLD
                     PVPQTEKIYKSKKLIHEIISEFLDAEATNFQAGKVIFERCRQILNEFDLVKEKKNEDL
                     VAEVYRFTGHSRSTYQDAISMAVFAASFAAMLGLDLQKIENAALAGLLHNLGLAQMPA
                     SATGKNLSELTAEELGEYKLYPDRSVILIKSKKVPLPPETSIAIVQHQENSDGSGFPH
                     GLDSRRLDPLGKVLRLAMRFLELTSLMDDQPGMTPTAAIAQIKEECLSDKPWTDLVVT
                     TQIFKKLGT"
     gene            complement(15738..16946)
                     /locus_tag="VIG33_04575"
     CDS             complement(15738..16946)
                     /locus_tag="VIG33_04575"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEY8270139.1"
                     /translation="MNIQSHPHWPALEKVYHILVENGHKAYLAGGCVRDALLGLQAND
                     LDLATDATPETIEKIFPKTVAVGKSFGVMLVVQEGITFEVATFRSDGLYADGRHPEVV
                     VYTTPQEDALRRDFTVNALFYDIGQGRVLDYVGGLKDLVDKKIQAVGNPEERFKEDHL
                     RLLRAVRFAAQLGFEIESETFSAVKRLATLVKTVSAERVHEEMFKLLKAKNSFAGLQL
                     VQESGLGKVLFPGWQNIFLKSQQEFRILFASDQKDEAFLWYSFLAPWALQKNLEWEWI
                     LGNYKFSRQLHKTLKKSLEHLENPKHFFLAARGEQLSLLGDEGVRLFARVCGLLGIEK
                     LAADRLMKQWLEWGEKMPDPWIRGEDLKDRLEGRELGVWLHRFFVWQLESKFSSREEM
                     LAQVPSFLNI"
     gene            17018..18073
                     /gene="queG"
                     /locus_tag="VIG33_04580"
     CDS             17018..18073
                     /gene="queG"
                     /locus_tag="VIG33_04580"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEY8270140.1"
                     /translation="MKFDELQNFLDNKAKALEFSHYGISSLSRPLTFEFYKNWLAEGL
                     HGKMEYLVTHAPFKEKPQIKWPKACSAFVFAVPYYPHPAPAAPFPLKAARVSLYAQGY
                     DYHHWFLGKLQSICDELKALFPEEEFIPMTDSSPVLERDLAFRAGLGWVGKNTCLIHP
                     KKGSLFFIGEIYSSLSFKLEPQFVHDFCGNCTRCIDICPTEAISEARKLDARKCISYL
                     TIESREIPPVELREKMGDWFFGCDLCQTTCPWNQKAFKNQLNFEKIEFHNPEREKLLE
                     EELRWILSSSGKQIERSFQGLSLSRAGGFGLKRNALIVAGNRKISALKKEIEALDSHA
                     KLGELAKWALNKILTHP"
     gene            complement(18059..18547)
                     /locus_tag="VIG33_04585"
     CDS             complement(18059..18547)
                     /locus_tag="VIG33_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270141.1"
                     /translation="MIESKAIILDLIGLLEDIEGDFSKVSQLEEYGQKVDRIMGGAKS
                     LALLVSLEHPLHMVADYADLCKAVGYKASQIKGNPQFYDICVALLLDATETLNDWIEG
                     LGKDTTEDLKSSFSKMFLERLRWVSSQFSADVRASVDAQSGAKKMAQNEIDDLLRKLG
                     VG"
     gene            18620..19549
                     /locus_tag="VIG33_04590"
     CDS             18620..19549
                     /locus_tag="VIG33_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY8270142.1"
                     /translation="MDYVSIRVSTLRGDQKIDFNAYVKINDKMILYLRRGDSFEGDRL
                     KRLKEKKLKKMFILNDEEGPYREYLQKNIEMAYDEKSGKNINARAEIIQGQQQSNTEE
                     LFEDPGNIESYNNTKDAAGKYVQFLLGNSKAVSSIMNITNDDCNIAHHGVTVSTLSVA
                     LAQKLGLVDPKKTQLLALGALLHDFGHYESGLSLNRPLAQFNKEETALYKNHPSVGSK
                     KVQDKKHFDPAVINIISQHEECIDGSGFPKGLRENQLDPLAVIISSANALDRMITFEK
                     VPRAEAGKKIMIDQVGHHPLNHIQLLSEILKTI"
     gene            complement(19550..20110)
                     /locus_tag="VIG33_04595"
     CDS             complement(19550..20110)
                     /locus_tag="VIG33_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270143.1"
                     /translation="MKKIFLSSLIFASTMAFTGIAQARGGGSGILLDLNLYYGSVKVD
                     DVNSGVTTHQSDSASAIYDIKLGYLMGEGLYLGGIYTSRSESALNASGTSGSATGASL
                     GYMGSGGFFLMGHYLLSATEGAYKNGTGLQVDLGYKAGVSSGWLVGGELTYRSITYKK
                     NATLSSAFEAREVTEVLPLVSVGYIF"
     gene            complement(20130..20960)
                     /locus_tag="VIG33_04600"
     CDS             complement(20130..20960)
                     /locus_tag="VIG33_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270144.1"
                     /translation="MAQTQELRQLKDYDVLGVGACRRENLLNDLGAKVLLTHSYLIPA
                     SQLPLYASILQETLQWLNPQEVREGFAFPEASQSPTEFLFQLLLEDFQKNFPKTSLYL
                     NSELRESMKSYLLEFPWKGPLLSDHFRYFPLFLKRKFQDSRLYLMAQKEWLCSYLSFA
                     DFGGSTLQTGRIFVNPSLQVLHSGVEIQEVQLKPGLSIFYYDFFQRKVREYQVSAEEA
                     AVMDLLQEERVYNLDQLVEQLLIMELPVSFSREEWRKKLVKLKSESILIDSGLGRISN
                     "
     gene            21009..21734
                     /locus_tag="VIG33_04605"
     CDS             21009..21734
                     /locus_tag="VIG33_04605"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEY8270145.1"
                     /translation="MIYNLAEKTWPMIEEYLKNKKTVIIPLGSTEQHGPNGLLGTDFL
                     SAWEIAKKVGQNTRTLVSPPLCFGMAQHHMAFPGTMSLTPLTYIQVVSELFQSLAHHG
                     FREFTVINGHGGNIPSINAAFSQVLGTDQEFIFNLINWWHLPEVQAYEKEHFGNANGF
                     HATCGEISVTMHTHPEAYKEIPSNFKFAPTPLQYSWPMSPHYFKKTFHDGRMGSDPSL
                     ATHKHGEKIFNIAVDAISQKFAK"
     gene            21801..22379
                     /locus_tag="VIG33_04610"
     CDS             21801..22379
                     /locus_tag="VIG33_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270146.1"
                     /translation="MKYIFFLLTMFSIQWVFGVQVFEGYLTKNDGNYYFTPASADSFY
                     KVHALTHNVEENLKRFENGDFIIGSGSLEAANKKINIESVDYVGLRRLLGMWVGTDGI
                     MIFKDFSTMSFTPPFSGVKEDNKVRPSLTNYQKQFRYSMSPTDGNEWALFVSDDKGTS
                     FATMEFSNKNVIMKIFESESGKIVRTLKLERR"
     gene            22386..23363
                     /locus_tag="VIG33_04615"
     CDS             22386..23363
                     /locus_tag="VIG33_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HEY8270147.1"
                     /translation="MTWTTTADSQLFFTRNDSSDPRIGEWVTGLKLAQASDLSLIQSP
                     SWTLLGYPDDEGIDMNGGRRGASYAPDAIRTYFYKMTPSLMKPQPQSLLDLGNLRTDI
                     PLAERHERGKVIIKTALDLGHRTLSLGGGHDYGYADGAGFIEHCLAQQTGKGPRQNRP
                     VMINFDAHLDVRSTEKGFHSGTPFYRLLTQYEGQFDFVEVGLQNQCNSLEHLHWAQKK
                     GAQILLLQDIREVGLKKSLSSVIASLQGQPCCLSVDIDAFASAEAPGCSQSWTTGLHI
                     QEFLPTLQMLQSELTVKSLAIYEVSPPLDQDNRTSKLAALILHHTIFHV"
     gene            23381..>24307
                     /locus_tag="VIG33_04620"
     CDS             23381..>24307
                     /locus_tag="VIG33_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HEY8270148.1"
                     /translation="MKRGFGSDNHSGVHPRLLEAITKANVQHAPSYGTDEWTAKAEKA
                     FREHFGPKTEIFFVFNGTAANVLSLKASLKSFQACYCASLAHLNVDECGAPEYLTGAK
                     LITVPTLHGKLHVEDLKRVHLRRGDQHSVQGKVVSLTQPTELGTLYSMEELKEIIAWA
                     KSENLYVHMDGARLANAAVGLQKTFKEFTTDLGVDVVSFGGTKNGLMMGEAVLFLNPA
                     LAKDFKFYRKQMAQLPSKTRFISAQFVEYLSNDLWKEIATHSCEMAEKLYQGAKLLPG
                     VEVTQNRQSNAVFAKIPQPWVKVLREKYFFYVW"
BASE COUNT         6951 a         6035 c         4988 g         6333 t
ORIGIN      
        1 tttgaaaaag cccagcactg ctgtgaatat acccaccccc tcacagggaa aagatgtggc
       61 tctgagtttc aactgcaaat tgatcatgtc aggcctctgg cctgtggagg gagcgatgag
      121 atcaagaacc tgcgcgccct ccgtggtctt tgcaatagaa gggaagctca gagatgggga
      181 ctgcaaagac ccacagaaaa ttaaatccct agtcttattt ttctctaaga agatcccagc
      241 ttccgcttcg cgaggatttt tccgagttca actcctggct gatcaaaggc attgatgccc
      301 agatattctc cgagccccgc tactaccaac tcccagagca taaattgata gcccaggctc
      361 tcttcattca aagccttgaa tttcagagtc aaagtggaaa cgcctttttg atttaaagct
      421 tcttgagtgg aagccgcctc aacccctaaa agttccccca tgctacgccc ttgcagacat
      481 tgggtctcgg ggaactgcgc cttttgaatt ttcattcgcc ccccctcaga atccccgaaa
      541 cgctggaaga ccacgaactt gtccttggcc ccttccatca cttgctgtaa gatggaatgt
      601 tgatccgaag ccccaatagc gcaaagagga gtgctcacgc gcggagcccg gctgccatct
      661 cgtttagtgg ctttccccaa agactcggcc cacaattgct gaaaccataa tccaaagttt
      721 ttcatgcgag cattataaga ccaaagcaag gaaatccatt ctccgcgctc aaagctttgc
      781 acaaactgcg ccatcaaagt cacgaccatc tcttgatctt gcaaagcttg cagagcccct
      841 tgacgaatct tctccaaact cattcccgca aaagccgcag gcactaaacc caccgccgaa
      901 agaacagaaa aacgcccacc gacatcttga gggatttcaa actctgggac tttatgggcc
      961 cgcgcccatc tcgtcagaga actctctttc gtttcagaaa tcaccagaga atagtcagca
     1021 agttgaagat ctttttcttg ataaatttgc tgtaacaact cgagagcgca cagagactca
     1081 atggtggttc cgctttttga aacgaaagcc caacaggtgc ttttaaaact cgccatatcc
     1141 ttcagaagat ttgcgatctg actttcaaat tcgatggcat cgacattatc gacaaagaaa
     1201 atatttttta cctgcgccac ctcttgaatc actcgggttc cgagagagct cccgcctaaa
     1261 cccacgatga tgagattttt aaattgagag agtttctctt cgtacttttc cgcctgcaac
     1321 cagagctcac gacgctcagg caatttaaaa aaaccaattt cagatctttg aagaaatgct
     1381 ttatatgaat ctcgacactg ctttaaggtc tcttctttcg cagcatgaga ggcttgagaa
     1441 atttccatga acatacaaaa tccttttgaa taaaaaaaaa gagagatttt tcaatctccc
     1501 ttttcccgaa gtaaacaaca agatccatcc gagaacgttt taagtgtaga actttttatt
     1561 cgttttcgcc aagtccttta atggatttgc aggccggagt ctagcagcat ttctctgcac
     1621 cgcgtattca gccaattgag tctcgatata ggttaaaccc acgctatcag catactttaa
     1681 aagtccgccg cggaacggcg gaaagcctgt gcccataatc atagctagat ccacctcatg
     1741 cggtgtttca acaattttat cttcgaccaa agccctcgaa cactcgttga ccatggcaaa
     1801 aataccacgt tcaatacact ctttagaagt taaagggctg gttgcaggtt taagactcaa
     1861 tgctgcataa atagtttcat ccacttccag gcgttttcca gtctcggaat aagtataaaa
     1921 acccttgcca ttctttttgc ccagacggcc actcttttcc agaatgtcca tacaaggggc
     1981 catttcaacg cggtctccaa aagacttctt aaagatcttt aaaaccttaa tacaaacgtc
     2041 aagccctact tcgtccatca aagcaaaagg ccccatgggc atgccaaact ctttaacaaa
     2101 agccatatca acggttttaa tatccatgcc atcctgcaac aaaaaagcag cttcggccat
     2161 atatggcaac aaaaggcgat ttactagaaa tccaggtccg tctttaacta ccaccggcat
     2221 cttgcccatt tttttggata attcaaaaat agttgccacg gtttcgtcgc tggatctttc
     2281 cccacgaatc acttccacta agggcatctt atccacagga ttgaagaagt gcatgcctgc
     2341 aaaatactct ggccgaggat ggcctttaga catttcagtc acactcagtg aacttgtatt
     2401 cgtcaccaga atggcatccg ctcgcatttt gctggcacac tcagcgatca ctttttgctt
     2461 gatcgccata tcttcaacga tggcttcaac aacgacgtct aagttcataa aaccagcata
     2521 gtcagtagcg actgtcactt tatctatctt ctgacggtac tggtacttat cgatggattt
     2581 acgctttaaa agcttcatcc aaagatcact cgcatgcttc aaacctttac caagcgcttc
     2641 aggatttaaa tccttcatcc gcacttcaat gcctttatca gcagcaacgt aggcaatacc
     2701 tccacccatg gtgccggcgc ctaaaatccc catggatttc acctcgcggc ccttcacgtt
     2761 ctttcccgga acgcccgaag atcttttgac catctcggtg agataaaaaa catggatcaa
     2821 atttttgcta atttcagttg tcgccaaatc acagaagccg gtgcgctcca cagccaaggc
     2881 ctgttcacgg ccctcttgag tgttggacca gccataggtt ttttgaatca cttccaaagc
     2941 ttttaaagga gctggatagt gaccgcctgt cgccttcaaa acaccctctt tggctttttt
     3001 gaaaacgatc ccacgaccaa agaaactctc aagaacttta ttcatcgctc cttgaggttg
     3061 atattttttg accgctttgc tcgttccctt accaataatt tctttggccc actttgtagc
     3121 ttcatccatc aagatggcag gatgaacaac acgatccacc aagccgatct tagaggcttt
     3181 tttcgcattc acggatttgc ccgctaagat aatatccaaa gaagcctgga gccctacaag
     3241 tttcggcatg cgtacgcatc caccaaagcc cggcaggatt cccaattgaa tttcaggaag
     3301 gccgatttta gtcgaagaat cttccgtggc catgcgatag tcacaggcca ggatgaattc
     3361 acatccccca cccatgcagg caccattcac cgctgcgatg gtcggcatcg gcaaatcctc
     3421 aaccatattc atgacctctt gccccccccg caccgccgcc tcatattgct cacgggtcgt
     3481 catgttttta atttcttcaa tatcggcacc ggcaataaat attttacttt tcttcgaaac
     3541 gaagattaca gccttataag aagactttcc gagctctgta atcacgtctt tcattcgcgc
     3601 catgaccgga gtggaaaact tatttacttt ctctccgact agatccagtt caacaatggc
     3661 gatatcgccc tgagagacaa ttctgatact ttcttgcata ctcatagata ttacccttct
     3721 ctctcaagaa tcatagagcc accttggcca ccaccaatac acagagtggc taaaccgaat
     3781 tgaacatctc ggcgctgcat ttctttcatc aaagtcacaa ccaaacgagt tcctgtcgca
     3841 cccactggat gccctaaagc aatggctccg ccattcacgt tgacctgatc caaattgatc
     3901 tcgcccactt tttgcgacaa gcccaacttt tcctgagcaa atttatctga gtccatcgcc
     3961 ttcacgcagg aaagaacttg agccgcgaaa gcttcattca actcgataag acctatatct
     4021 tttaaagaca atttggcacg cttcaatgcc accggtgtcg aatacacggg gcctaatccc
     4081 atgcgctcag gctcaagacc tgcaaaaccg taagaaacaa ttcttgcaac cggtttatga
     4141 cccaagctct ctgctttttc tcgagacatc aacaacgcca tagcagcacc atcagtgatg
     4201 ggacaagaat tgcctgcggt gatagttcct gtttgtttat caaagaaagg cttcagctta
     4261 gccagagctt ccagagtttg actttcgcga ggaccgttgt cttcattgaa gacctcttta
     4321 taatctggag caatatagac cggcgcgagt tcttctttaa gtttggcttt attggctgac
     4381 gccagtttat gagagcgcag agcgaactca tcttgggttt gacgagaaag cccccactct
     4441 ttagcaagaa tttcagcggt ttgtcccatg ttaataccga caaatggatc cgtcagacct
     4501 tgcagcaccg aaataacagg aaaatattca ttccgagtat tcccttgcaa aagagcctta
     4561 atttgattca catccgcctt aaaaagcttc cacaacaaag gcaaagcttg ttgcggacct
     4621 ttggcggcaa agagcttatc gaaaagcgct tggattttcg tcggcaaaag cgtgggcatt
     4681 tgcgacatat tctcaacgcc accggcgatc accacatcca tagttcccga tttgattttc
     4741 tcataaccgt ttgaaatact ctcgagcgca gaagcacaat ttctgtggac ggtgtaagcc
     4801 gaagttttca tcggaatgcc ggcattcaaa gccaccactc gcgaaatatt caccgcatcc
     4861 accggatttc cggtatttcc aataatgacc tcatccacca cattcacatc gagattcgct
     4921 cgagcgatga gctctttgag agccactttc ccaagctctg caggatgaag agattttagt
     4981 tttgttccgg cttttgcaaa aggagttctt acaccttcaa caataaccac atcacgcgga
     5041 ttctttgaac gatttgcagt agtactcatt cttaccttcc tcgtgcgcac cacgagtatc
     5101 gatttttaat aaagtagttg gatattacag atttttagct tctggtccaa gctcttttaa
     5161 agggccaaaa tatgaatatt cctgcattga ccccttctaa ggacctttgt acattattat
     5221 tatattttat agaataatag ctctgctatg tttttcaaag aataatagct ctgcatacac
     5281 attaagcatt ttccccgcag aagctgacaa agcaatgcag gcaatgcagc agccaatgca
     5341 gctgtcaacg caatggagag gtcaatgagc gaaaaacttg aaggtcacca acaagggaac
     5401 caaatactaa gccctttgga acaagaagcc ttagactatc atgcaaaagg taaacccgga
     5461 aaaattgaaa tcacttctag caagccttgc aatacagaaa aggctctttc tttggcttac
     5521 tctccagggg tcgctgtgcc ctgcaaggtg atcgcgaaag atccggctaa agtttatgac
     5581 tacacgagca aaggaaatct cgtggcggtg atctctaatg gcacagccgt gttaggcctg
     5641 ggtaatatag gccctctggc ctctaagcca gtgatggaag gcaaaggcat tctctttaaa
     5701 caattcgctg gcattgatgt tttcgatatt gaaatgaaaa caaataccac cgcggaactg
     5761 attgcggcca tcaaagcact ggagcccacc ttcggcggaa ttaacttaga agacatcaaa
     5821 gctccggagt gttttgaggt cgaggaaacg ctcaaaaaaa tcatgaacat tccggttttt
     5881 catgacgacc agcatggcac cgcaattgtg tctggggccg ctcttctcaa cgcctgctat
     5941 atcacaaaaa gaaaactcga agacattcgt ctcgtggtca atggtgctgg cgccgcctca
     6001 atcgcctgct ccaatatttt catttcttta ggtgtgcgcc gtgaaaatct catcatgtgc
     6061 gacactcaag gcaccatcta caaaggccgc acggcgggaa tgaataaata caaggaggcc
     6121 tttgccgttg aaacggaagc ccgtaatctt cacgaagctc tgcagggagc cgatgtcttt
     6181 ataggtcttt ccgtcgcggg cgccttgact gcagaaatgc tgcaaggaat ggccaaagat
     6241 ccaattattt ttgccatggc caaccccgat cctgaaattg aacccgcaaa agccagagcc
     6301 attcgcccgg atgccatcgt cgccacgggc cgttccgact ttcccaatca ggtgaataac
     6361 gtgctgggtt ttccgagtat tttccgcggc gctttagaca cccgttccac gcaaatcaat
     6421 gaagagatga aattggccgc cgttcacgct ctcgccatgt tggcccggga agatgtgcct
     6481 gatcgcgtct ctgactctta cgggggccaa agcttcaaat ttggacggga gtatttaatt
     6541 cccaagcctt ttgatccccg cgtgcttttg aaagtagctc cagcggtggc aaaggccgca
     6601 atgacctcgg gagtggccac caaacaaatt accgattggg aggactacag cgaccgtctg
     6661 gaagccatcc aaggtccttc gaaggtcttt attcgttctg ccatccatag agtccgccaa
     6721 aacattgaag cccacggcgg agagcttcca aaaattattt ttcctgaagg gcatagcaaa
     6781 aagatcctca aagctctgcg caatttagtg gaagagcgca tttgtcagcc gattctttta
     6841 ggttatccgg aacgtattca cgaaaaaatg cataacatgg atttgaccga tactttaaag
     6901 catattcagg ttattcaacc ctcgaaccat cccaaattca aagcctatgc ccaagaactc
     6961 tatgttcgcc gcaatcgcaa gggcgtgaat taccgggaag cggaacgact gatgtcagac
     7021 cctaattatt ttgcgtcgat gatggtgcaa atgggagatg cggatggcat ggtgacagga
     7081 gccacgcaac attatgccga ctccgttcgc cccatcttcc acaccattgg catttccaaa
     7141 gaagcggtgc ctgccggttt aaatctggtt cttctcgaag atcgcttcct gcttttggcc
     7201 gacaccaccg tcaacatcaa tcccaccgcc gagcagtgtg cgatcattgc cctcgaagcg
     7261 gctcgaattg ccgagtactt tggcgtctcg cctcggatag cgatgttgag ttactcgaac
     7321 ttcactggcg gcgacggaac ccccgcaaaa atgaagagag ccgcagaatt ggtaaaaaaa
     7381 cgtcgtccgg acattcccgt ggatggcgat atgcaagcgg atacggccgt gaatgatgaa
     7441 atcattaaac gtatcttccc cttctgcgat ttaaaagatg ggggagccaa tattttgatt
     7501 ttcccaaatc ttgaatcgag taatatttgc tataaactgc tgcagcagat cggcaaagcg
     7561 gaagtcatcg ggcctttctt aatgggcgtt cgcagatccg cgaacgttct gcaaagaacc
     7621 accaccgtcg atgcgattat caactccgtg gtgctgacaa ccttagaagc tcaatacatt
     7681 aaagacgcca tcaaaaagaa aaaatagaac ctaaatttag agcatatcca ggtcttgtaa
     7741 taagacattt ccacccctaa aaaaaggggt ggaaccagtg aaaatttgaa gtattttccg
     7801 cttgctccaa cgaaatcctg tgatagcatt agagcttgta gggagagtct cgagcagaga
     7861 ctccttgaaa gataaaggtg ggagatcaag agatttgact caaaaccaag tccatatcaa
     7921 aaccagtgat cgggccattg tcgtcggtgt agggttaaaa accgagccta tttctgaaat
     7981 caaagaaaat cttttagaac tggaagagct ggtcagcgcc gctggcggtg agatcgtggg
     8041 ttctgtcatt caaatgctcc ccgcgtggaa tccggcaact ctgatcggca ctggcaaagt
     8101 ggatgaaatt cgcaatatgg tgagcgagtc ccaagccagc gtggtggtga tcgaccacca
     8161 gctgacgggg gtccaaagca gaaacttggc ccaggccatg ggcacccggg tgattgatcg
     8221 caatcaattg atattggata ttttcgctca gcgggcgcag acctttgaag gaaaacttca
     8281 agtggaactt gcgcaacttt tggatcaaat gccccgcatg gtcggggctt ggatggaatc
     8341 gctctcccgc caaggtggag gtatcggaac tcgcggtccg ggcgagaccg cccttgaaag
     8401 tgaccgacgc agaattcgcg agaaggtcgc aattattcgc aaaaaattag attccgttcg
     8461 taaaaaccgc gctcaacatc gcagctctcg tcgccgcaac gaaatcccca gctttgcctt
     8521 gattggttat acgaactctg gaaagagctc gatcttaaat cgtctttccg gctcgcaggt
     8581 tttggcaaaa aaccaagtct ttgcaacctt agatcccacc acgcgaaaaa tatttttacc
     8641 cgaaggcccc ccggccgtgg tcaccgacac cgtgggcttt attcgcaaac ttccaaccca
     8701 attgattgaa gcctttaaag ccacgcttga agaaagtgcg gaagccgatg tgcttttaca
     8761 tgtgatcgat ctttcttcgc caaatatgct ccgccaaatt gaagtggtgg aatctcttat
     8821 tcaagagttc aactggcagg ataaaaaaat catccatgtc tttaataaga ccgatgccgc
     8881 ccctatcgaa agacaatttc aagtaaaact ttatccgcga gttttcgtca gtgctctgac
     8941 cggacaaggc atggagcagc tcaaaaaatt gatggtcgag agtgtgcaag aaatgcaaac
     9001 ggatgtgcag ctttacttcc cgcgctctca agaatttaaa atttatgatc tggaccgcga
     9061 gacaaagatc ctgcgtaaag aaaccgcgac cgaaggcacg gtctgctata cacaactcac
     9121 gccggctttg atttcgcgtt ggaaagatta tctcgttaag tagacgccat atgtagaagc
     9181 tctaagttgg cactctgatt ccacaacttc ggcctagtct agtttccggc ttgaaccttc
     9241 attgtcctga gaatttcgcc gatagtaata tcatgcgctt caactcctgc ttttgtatca
     9301 gtctcataat tctttgcagc gcaacaaccg tccttgctct tgagagccat tcctcagcag
     9361 catcagtcga aaaatgcatt aacggcgatt acaacgaaaa tacagacttt attaaatcga
     9421 tcgaggactc gatagacccc ttgagattga cctatttgac tgccaagtcg gagtccgtca
     9481 cccccataaa aaactcctcc cacccccctt gcctggagac ggatacaaat aagaaacaac
     9541 tcccttatct tcaggagatt gccaaggtag ctactcagaa aattaattct ctatttgccc
     9601 ccgaacccgc cgcgaaaatc aatcgagaat gcattgctgc cgcaatgaag cgcaaccctg
     9661 gagacaccgg aatcctctgc accaccccta aaagtatttc aagaaagaac aaaaccaacc
     9721 aatgcatcaa cgacaacacc gttgattata ttcactttgc cgtgaacagc gccattcagt
     9781 gtatgtcttc agacgatccc atcgatagcc gggtcctttt aaaaaagttc aacaacgaaa
     9841 ccgcatttca tccttccatc gcctcgaaag gcggagtagg ccttggccaa atgacaagca
     9901 ttgccgtcaa cgaaatctct caaggacagg gcagatatat actagagaac atcgccgcta
     9961 gcagaaaaac agagtgcaaa ggtttccgcg acatcgccgc aaaggattta gaaaaaaacc
    10021 ctcggattaa ttctaaaaac ttttgccctt gggtcagtcc aggcgacggc ttagcgagaa
    10081 atcttattta tagcataggc tactacttaa ccctgcggga tcaatatatc atccccgccg
    10141 tggaaagaag gtcttcttct ctgagtagct ccaaagatct tatcaatgac ctgacgaata
    10201 tcagttatgg ccctgaaggc cttaagcaag tggagtggat tttgcaaaag taccgcgtga
    10261 ataataaaac aagcacgcaa accctccagt cccaggttaa caaaaatagc aaatatcttg
    10321 aagatacaaa gaacaagatg cacgagctga cttgcatcag aaaagatctg aacccggctg
    10381 gcaaggaatg cgagaactat aaattaacta ttgacgaact gaaggcaaac acatgcattt
    10441 ccaattaatt ttcctgttat tcttattccc tgccttttct tacgccactc ttcagacgac
    10501 agcggaattt atggagcaat cccataaaac ccgagaaaca ctttccaaag atgcatcttt
    10561 taaaaagata gccgatgaac tgcagacatt tgaaaagtcc ctcaacacag caattcaaga
    10621 atacgaaaaa gattcaccgc ttgaaggggg cgatgaagaa gaaaaggtct ttaaattatc
    10681 tttcggattc aaaaatattt tcaatctagc aaagcaaaaa tccgctagca aagaagcctg
    10741 cgaaaaagcc aaacatcaaa ttgaatttga ggacaaaagc ggccttgacg aggatgcaaa
    10801 actcagcgaa gccgccgaag aagcattaaa gtggctggag cttctctgta aaacttttta
    10861 atctataccg atgacgacat tggactgctt tgcaacccac tgaacaagtt tcatcttctc
    10921 tttttggtcc atgaaatcca agcacaagtc cttggcttta ttattatttt caatgaaaac
    10981 tttttcctga caaaaaggat ttaaatggcg aaattgctcc tgagttatac tcttggaaaa
    11041 actctgaatc tcctttacca acactgaaaa cgcttgatgt ggcatcacct tttcgagctg
    11101 ccttttctgt ggagactgat agccaatgag gtaaacttct ttgttttgaa tttttctctg
    11161 cctcaaccaa agaatttcat gacgatcaga tcccaaagcc actgaatagc gagtctctgc
    11221 aaagctacaa aaaggcaaca ccagcgcaga aaagaagatt aaatatttca tttttctcct
    11281 tcttgcattc taagctcgga gtcattgcgt gacgcccccg ctgaacccat tctcgtggaa
    11341 aaacttaaat gatcgaaaca ggctggagct tttctctggc agtcataaat ttttgccatt
    11401 cctggaagat tgagcatgat tttttttacc ctgtcactat tctcagggtg tgaatcggaa
    11461 aagctgcttg atgaaaataa agtttctaac gttttaggag ccaaggactc gggcctcttt
    11521 accactgaat tatttaaaga gcatccatca ataaaacgaa tggccgccac tttttcctct
    11581 tcatttttaa aaggattctc gattatgtac ttttcggcga tgaccgagcc gactacatcc
    11641 gccattgttt cctccatctg tgatgccgga gccaatcctc cgcagacaga ttctttttta
    11701 tataagcctc tatttcggct ggtttgatcg attgcttttt tgcatcttta tccgttggcg
    11761 gccaaggaat ttctaccgcc tgatctggaa ctaaagccaa tccagcaatc tgacccgcgt
    11821 acatcatttt aaaacgtgtt gaaaacgatg tgctctccaa ggcatcaata atctcagact
    11881 gattgtagat caatttatca taatccatgc cgcctatata gccattccca cctatccccc
    11941 cacctggagg attatcaaga tttgggagtt gtggcttttg gggatctaca aaaagaggat
    12001 tataaaaagt acttccatca gcaagagtga tgacacgttg gctagctcgc tcacgaaaga
    12061 aatcgcaatt tctcgtcaca gtattggagt cacagaccga ctttcccttt gacctcgctt
    12121 tggaatcatt tttttcagcc gcaaaggaca tcaaaagaaa aataccaagc aactttacaa
    12181 gactcatcct gacgtcctcc ggtcagtatt tttaactgtt ctggcgactt ttcaagcgag
    12241 cttcacgacg ttttctcgcg gcctcaaaac gccgcttctc atccgccgtc tcaagaacca
    12301 atgcaggaac ttccgcgggc tttccgttcg aaccaagagc gacgaaggtg gtataagcag
    12361 aggccgtgtg aaacacctcg ccggtgcggg gattttcagc atccactcgc acgccgactt
    12421 ccatggaagt gcgggaggta aaattgacgg aagctttgag attcaccacc caccctttat
    12481 agacgggagc gataaaacta agggagtcca tgcttgcggt gacgacctct ttattggaat
    12541 gcctttgagc tgcgatcgca gcggcaatat cgatccaaga catcacagtg ccaccaaaga
    12601 tagaacccat ggcattgata tggcttggta aaactaattg ggtcattatc acttgggatg
    12661 ctgaaactgg ttttgcattc cccatgataa tgacccctca actttcaaac aaaaatctag
    12721 tgttggaaac taaatgcctg cgccgatgtc gccattacta ttaccagttc cagtgcggcc
    12781 aatgccattt tgtgcacccg gaagagtcgt cgcaacaccc gctaccgtcg agtgaccagc
    12841 agggatcatt tgagtggctt gattattata agccgctgct gcaactcctt gattgatatt
    12901 catctcggta cgagtttcgt tctctacgaa tccaagatgc ggcaaacacc atacgatcaa
    12961 ttgagcgcga gatttcacgt tcattttttt gtagatattt gtaaggtgaa atttcactgt
    13021 tttttcagtg acaaaaagct ggttcgcaac ttccttgttg gacaagcctt tgcttactaa
    13081 ttcagcaact tcagcctcac gattagaaag acctttttga atcaggacat ctctgagcat
    13141 ccttgctccc ttcccgttat tgttgttctt cggtttatgt tgttttcagt ttttgcttaa
    13201 aaattagtct caaaacatct ggaacttgtt tgaaaagaac ctctatgttc gtcccaaaaa
    13261 gagccacact acctagattc aagtcttaca agaaattgtc tttttagcaa gacagttgga
    13321 ctctagtttt aaaaaactag accgaaaaga gataagaatt taaaaattaa ccggcgagag
    13381 gatattatgt ttcctaacga tattaaattt ttagtagtcg atgatttcgc aaccatgcgc
    13441 aaaattatca aaaaagttct gaacgagctc ggctatacca atatcgaaga atccgatgat
    13501 ggaaagaatg ccctgcctat acttcaggca gcagtcagca atggtcagcc ttttggcttt
    13561 ataatatcgg actggaatat gccaggcatg accggaatcg atcttttaaa agcctgcaaa
    13621 gcggatgcga acttaaagaa tttgcccttt atgctagtca ctgccgagag tgaacaaaaa
    13681 catattttgg aagcagccaa agccggagtt tctgattatg tggtaaaacc ctttaacgca
    13741 gcaaccttaa aggcaaaaat ggagagaatt tatcaacggc attcgacacc accggcagcg
    13801 aaagcctctt gatagcatga ataacccctt ctccaattct cttaaaagac tcaagctaag
    13861 gagcttccga tgtccgctgc acccaaaata gttccagagg ttaacccgct ctttgataag
    13921 aaattgcttc aagcttttgt tgacggcgct cttaaaactc tggccacgat cgctcaaacc
    13981 gaagcaaagg tgggaaagcc tcatattgaa accagactca gcgaaaaatg cgaaatcgcc
    14041 ggcatggtgg gaatggtggc tcctccctta aaagggacct tgctcattag ctttaccaaa
    14101 gaatccattt ttcaaattat ggaaaatatg ctcggggaaa aacacaccga aatcagtaac
    14161 gaaatttcag atgccgtggg cgaactcact aatatgatct atggaaccgc caaaacagct
    14221 ctgaatcaaa tgggctataa ctttgaaatg gcaatcccca ccgtgattcg tggggaattc
    14281 acgctttcca agtccaccaa aggagctact ctggttattc cttttgagct caagaataat
    14341 tcggcctttc acatcgctat cactgtacaa acctaacagt ttaaaatgcc aaaaaaaccc
    14401 attgattctc aaacagacgt cctcatcgca agccagagag aaaatttctg gcaagacgtc
    14461 cagataatac tcaaaggatt ttatccctac aaacttcact ttgtgaggag ctccgaaagt
    14521 atcattaatc caacggaaga aataactccg ctgttgacct tagtggacgg ctttggcggc
    14581 acaccgcaaa cctgtgaatg ggtgcaaagc gtgaaaatgg ccttccccaa ctgccctctc
    14641 attgttttat atggccccga agacagcttg gacttttcct tggttaaaaa aaatggcgcc
    14701 aacttcatca tgcatacgaa ttatgatcga gagtttattt ctgacatgat cctcaacctg
    14761 gcgcccgtgg aaatacaagg gacggacctc cctacttcag ctctgcttcc tattgatatg
    14821 aaagatatcg atccggaaga acctattaat tttgatattt ttattcattt accaacaaat
    14881 cataaaagtt ttcgcgtgcg taaaaatggg ggtaaaatcg atccccggct tttggaaaaa
    14941 tcctctgaaa ctcaccaacg tctttatatt aagaagaccc agatcaagcc tttttttgaa
    15001 tatgcccgca ctgtcctcac gatgagaaat ctcttggatc cggttccgca gacggaaaaa
    15061 atatataaat caaaaaaact gattcacgag attatttcag aattcttaga cgcagaagct
    15121 actaactttc aggcgggcaa agtcattttt gagcgctgcc gccagattct taatgaattt
    15181 gatcttgtta aggaaaagaa aaacgaagac ctcgtggctg aagtctatcg atttaccggc
    15241 cactctcgca gcacctacca agatgctatt agcatggcgg tcttcgccgc aagcttcgcc
    15301 gcgatgctgg gtcttgattt gcaaaaaatc gaaaatgcgg ccttagcagg tctattacat
    15361 aacctaggct tggctcaaat gcctgccagc gccactggta aaaacttaag cgaactcacc
    15421 gccgaagaac ttggcgagta caaactttac ccagatcgat cggtaatttt aattaaatcc
    15481 aaaaaagtcc ctcttcctcc agagacttct atcgccatcg ttcaacatca agaaaactct
    15541 gacggcagtg ggtttcccca tggtttagat agcaggcgat tagatccctt gggcaaagtt
    15601 ctacgtttag ctatgcgatt cttagagctg acttccttga tggatgacca accgggcatg
    15661 actcccacag ccgccattgc ccaaattaaa gaggaatgcc tttccgataa accttggact
    15721 gatttagtgg tcacgactca gatatttaaa aagctgggta cctgagcaag catctcttct
    15781 cgggaagaaa acttactctc caattgccag acaaaaaacc tatgcaacca aacacccaac
    15841 tctcgtccct caagacgatc tttgagatcc tctccgcgaa tccagggatc aggcattttt
    15901 tctccccact ccagccactg tttcatgagt ctatcggccg ctaatttttc aatccccagc
    15961 aggccacaaa ctcgcgcaaa caaccgaacc ccttcatcac ctaaaagaga caattgctcc
    16021 cctcgggcgg caagaaaaaa atgcttgggg ttttcaaggt gttccaagga tttcttcaag
    16081 gtcttgtgta gctggcgtga aaatttataa tttcctaaaa tccactccca ctccagattc
    16141 ttctgcaaag cccagggggc gagaaaagaa taccataaaa aagcctcatc tttttgatca
    16201 cttgcgaaca atatccgaaa ctcttgttga gattttaaaa aaatattttg ccagcctgga
    16261 aaaagaactt tccctaatcc gctttcttgc acaagctgaa ggcctgcaaa gctattttta
    16321 gccttaagaa gtttaaacat ttcctcatgc acacgctcag cactgactgt tttcaccaga
    16381 gtggcaagtc ttttcaccgc gctgaaagtt tcgctttcaa tttcaaatcc taattgagcc
    16441 gcaaagcgca cggcccttaa aagacgtaaa tgatcttctt taaatctttc ctctggattg
    16501 ccaacagctt ggatcttttt atcgactaaa tccttaagac cacccacata atctaaaact
    16561 cgaccttggc caatatcata gaaaagggcg ttcacggtga agtcgcgacg aagggcatct
    16621 tcttgtgggg tggtataaac cacgacttca gggtggcgcc cgtctgcata aagtccatcc
    16681 gaacggaagg tcgcgacttc aaaagtgatt ccctcttgca caactagcat aacgccaaag
    16741 gatttcccca ccgccacggt cttcggaaaa atcttttcaa tggtctcggg agtcgcatct
    16801 gtagccaaat ccaaatcatt cgcctgtaac cccaacaagg catcccgcac acagccgccg
    16861 gctaaatagg ctttatggcc attttcgacc aatatgtgat acactttttc taaggctggc
    16921 caatgtggat gagattggat attcattcat ttaagagtaa cctgccccct ggaagaaggg
    16981 aaatctcttt taaaatccct tttactgaag agagaccatg aagtttgatg aactgcaaaa
    17041 ttttttagac aataaagcca aagcgctgga attctcgcac tatggaatca gctctttaag
    17101 tcgtcctctt acttttgagt tctataaaaa ttggcttgca gaagggctcc atggcaagat
    17161 ggagtatctg gtgacccatg ctcccttcaa agaaaaacct caaataaaat ggccaaaggc
    17221 ctgttccgct tttgttttcg cggttcctta ttatccccac cctgccccag ccgcaccctt
    17281 ccccctcaag gcagcccgag tcagcctcta tgcccaaggt tatgactacc accactggtt
    17341 tctagggaaa ctacaaagta tttgcgatga gctaaaagct ttgtttcctg aagaagaatt
    17401 catcccaatg acagacagca gtccggtgct cgagcgggac ctggctttcc gtgccggcct
    17461 cggctgggtc ggaaaaaaca cttgtctcat tcatcccaaa aagggcagcc tgtttttcat
    17521 tggagaaatt tactccagcc tttcctttaa gcttgagcct caatttgtcc atgatttttg
    17581 cggaaattgc actcgctgca ttgatatttg ccctactgaa gccatctcgg aagcccgcaa
    17641 attagacgca cgaaaatgca tttcttatct gaccattgaa tcccgcgaaa tccccccggt
    17701 ggaactccgc gaaaaaatgg gtgattggtt cttcggttgt gatctttgtc agaccacctg
    17761 tccatggaat caaaaggcct ttaaaaacca attgaatttt gaaaaaatag aattccacaa
    17821 ccccgagcgg gaaaaactcc tggaagaaga gctgcgctgg atcctgtcaa gttctggaaa
    17881 acaaatagaa cgtagttttc aaggtctttc gctgtcgcgc gccggtgggt ttggacttaa
    17941 acgcaatgct ttgatcgtcg ctggcaaccg caaaatatcc gctctcaaaa aagaaatcga
    18001 agccctcgac tctcatgcta aactagggga acttgcaaag tgggctctca ataaaattct
    18061 aacccacccc tagctttcgc aagagatcat caatttcatt ttgagccatt ttcttggcgc
    18121 cactttgcgc atctacagag gcccgaacat cagcactgaa ctggctggac acccagcgaa
    18181 gtctttcaag aaacatttta ctaaaggaac tctttaagtc ttctgtggtg tcttttccca
    18241 atccctcaat ccaatcattc aaagtctcag ttgcatccaa caagagggcg acacagatat
    18301 cataaaattg aggatttccc ttgatttgag aagccttata acccacggcc ttgcagagat
    18361 ccgcatagtc agccaccata tgcagaggat gctctaaaga aactaagagc gctagacttt
    18421 tagctccgcc cataattcta tccacttttt gaccatactc ttcaagctgc gacaccttgg
    18481 agaagtctcc ttcaatgtcc tcgagaagcc ctataaggtc cagaatgatc gctttggatt
    18541 caatcacaaa atcttttatt atatctttat ctatgtccga catagtatcg agaataacaa
    18601 agtgaagggt tgaatagcaa tggattatgt ctctatcagg gtcagtactt tacgcgggga
    18661 tcaaaaaatt gatttcaacg cctacgttaa aattaacgac aaaatgattc tctatcttcg
    18721 tcggggtgat tcctttgagg gggaccgact caaacgcctc aaggagaaaa aacttaagaa
    18781 gatgttcatt ttgaatgacg aagagggccc ttaccgcgaa tatttacaga aaaacatcga
    18841 aatggcctac gatgaaaagt ccggaaaaaa catcaacgct cgagcggaaa tcatccaggg
    18901 acaacaacag agtaatacgg aagaactgtt tgaagatccg ggaaatattg aatcttacaa
    18961 caataccaag gacgccgccg gaaaatatgt gcaattcctc ctcggcaata gcaaagccgt
    19021 ctcttccatc atgaatatca ctaacgacga ttgcaacatt gcccaccacg gagtcaccgt
    19081 ctccacttta tccgtggcct tggcgcaaaa actcggcctt gtcgatccga aaaaaactca
    19141 gcttttggct cttggagccc ttcttcatga ctttggacat tatgagagcg gcctttccct
    19201 caatcgccct ctggcacaat tcaacaaaga agaaacagcc ctctataaaa atcatcccag
    19261 cgtaggctcc aaaaaagtcc aagacaaaaa acattttgac ccagctgtca ttaatatcat
    19321 ctcgcaacat gaagagtgta ttgacgggag cggctttccc aagggactgc gcgaaaatca
    19381 actcgatccg ctcgccgtca tcatctccag cgccaatgct ttagatcgca tgatcacctt
    19441 tgagaaagtt ccgagagcgg aggcgggtaa aaaaattatg attgatcaag tcggacacca
    19501 tccccttaat catatccaat tgctcagtga aattttaaag actatttgat cagaagatat
    19561 agcctacgga aaccaggggc agcacttctg taacttctcg agcttcaaag gcagaagaca
    19621 aggtcgcatt ctttttataa gtgatacttc tataggtcaa ctcgcccccc accaaccagc
    19681 cgctgctgac gccagccttg tatcctaagt ccacttgcag gcccgttcca tttttatagg
    19741 caccctcggt ggcactcagc aaataatgac ccatcaaaaa aaatcctcct gatcccatat
    19801 accccaaaga ggcacccgtc gccgagcctg aggttcctga tgcattcaaa gcactttccg
    19861 aacgggaagt gtaaatacct cctaaataaa gaccctcgcc catgagataa cctaatttta
    19921 tatcatagat agcgctggcg ctgtccgatt gatgagtggt caccccgcta ttcacatcat
    19981 ccacttttac cgacccataa tataaattca gatccaaaag gattccagag ccccctccac
    20041 gagcttgagc tatccccgta aatgccatgg tcgaagcgaa aataagagat gataagaaaa
    20101 tctttttcat aaatactccc tcactttatt taatttgaga tccttcccaa acctgaatct
    20161 ataagtatac tctcactctt taatttgacc agcttttttc gccactcttc cctcgagaaa
    20221 gagaccggca attccattat taaaagttgc tcgaccagtt ggtcgagatt gtacactcgt
    20281 tcctcctgaa gcaaatccat caccgccgct tcctcagcac tgacttgata ttcacgaact
    20341 ttccgttgaa agaaatcata ataaaaaata gaaagccccg gcttcaattg cacttcttga
    20401 atttccacac cactgtggag aacctgcaaa gaaggattca caaaaatacg ccctgtctga
    20461 agagttgaac cgccaaaatc agcaaaactt aaatacgaac aaagccactc tttttgcgcc
    20521 atcaaataaa gacgcgagtc ctgaaatttc cgttttaaaa agagggggaa gtagcgaaaa
    20581 tgatcactga gaagcgggcc cttccacggg aactcaagaa gataggactt catcgactcc
    20641 cgcaactccg aatttaaata gagagatgtt tttggaaagt ttttttgaaa atcctcgagt
    20701 aacaattgaa aaagaaactc tgtcggggac tgagaggcct cggggaacgc aaaaccttcg
    20761 cgaacttctt gcggattgag ccattgcaac gtctcctgta aaatggatgc ataaaggggg
    20821 agctgacttg ccggaataag gtaagaatgc gtcaataaaa ccttagcgcc taaatcattg
    20881 agcagatttt cacgacgaca tgctcccaca ccgagcacgt cataatcttt cagctgacgg
    20941 agttcttgag tctgtgccat gattttatat tgtacaattt gaaatctttt aagcgcaagg
    21001 agtgttccat gatctacaat ctcgccgaaa aaacctggcc tatgattgaa gaatatctca
    21061 aaaataaaaa gactgttatc attcccctgg gttctaccga acaacatggt cccaatggct
    21121 tgcttggaac tgatttttta agcgcctggg aaattgcgaa aaaagtcggc cagaacactc
    21181 gaaccctagt gagccctccc ctttgttttg gcatggctca acatcatatg gcctttcctg
    21241 gaactatgag cctcaccccc ctgacctaca ttcaagtcgt cagtgaactt tttcaaagtc
    21301 ttgcgcacca tggctttcgt gagttcacag tgatcaatgg acatggtgga aatatccctt
    21361 cgattaacgc cgcattttcg caagtgctgg ggacagatca ggagtttatt ttcaatctca
    21421 tcaattggtg gcatttgcca gaagttcagg cctatgaaaa agaacatttt ggcaatgcca
    21481 atgggttcca tgccacctgc ggggaaattt ctgtcacaat gcatacacat ccagaggcct
    21541 acaaagaaat tccctctaat ttcaaatttg ctcccactcc ccttcagtat tcttggccga
    21601 tgagtcccca ctatttcaag aagacctttc atgatggacg catgggttca gatcctagtc
    21661 ttgcaacgca caaacatggt gaaaaaattt ttaatatcgc agtcgatgca ataagtcaga
    21721 aatttgcaaa ataacacaag gagctaaact agagttttta tttttgaccg agcgcttcgg
    21781 ccatgcaaga agtggatagt atgaagtata tattcttctt attaactatg ttttcgattc
    21841 aatgggtttt tggcgttcaa gtctttgaag gttatttaac caaaaatgat gggaattatt
    21901 attttactcc cgctagcgct gattcttttt acaaagtcca tgccctgacc cacaatgtgg
    21961 aagaaaattt aaaacgcttc gaaaatggcg acttcattat cggaagcgga tccctggagg
    22021 ccgctaacaa gaaaataaat attgaaagcg tcgactacgt cggcctgcgc aggcttttgg
    22081 gaatgtgggt gggcactgac ggcatcatga ttttcaaaga tttttcaacc atgagtttca
    22141 ctcctccctt tagcggcgtt aaagaggata ataaagtcag accgagtctt acgaactatc
    22201 aaaaacaatt tcgctacagt atgtctccga ccgatggaaa tgagtgggct ctgtttgtct
    22261 ctgacgataa aggaactagc tttgcgacca tggagttctc gaataaaaac gtcattatga
    22321 aaatttttga gtctgaaagt ggtaagattg tacgcactct caaacttgaa agacgttaag
    22381 accctatgac ttggaccacg acggcggatt ctcaactttt cttcacacga aatgactcct
    22441 cagacccccg catcggcgag tgggttacag gcttaaagct ggctcaagct tcagacctca
    22501 gcctcatcca gtctccctct tggacgcttt taggttatcc tgatgatgag ggcatagaca
    22561 tgaatggggg ccgcagaggc gcttcttatg ctccggatgc cattcggact tatttttaca
    22621 agatgacacc ttcactgatg aaacctcagc ctcagtcttt gctggatctt ggcaatcttc
    22681 gcaccgatat tcctttggct gaacgccatg aacgcggcaa agtaattatt aagaccgctc
    22741 tggatctagg acatcgcact ttgagtttgg ggggtggcca cgactatggc tatgccgatg
    22801 gtgccggctt tatagagcat tgccttgccc agcaaactgg taaaggcccg cgccagaacc
    22861 ggccagtgat gattaatttt gacgctcatc tcgatgtacg ttccacagaa aaaggtttcc
    22921 acagcggaac tcccttctac cgtcttttaa ctcaatacga aggtcaattt gattttgtgg
    22981 aagtgggctt gcaaaaccag tgcaatagct tggaacatct tcattgggct caaaaaaaag
    23041 gagctcagat tttgcttctt caagatatac gcgaagtagg acttaagaaa tccttgagct
    23101 cggtgatagc ttctttgcag ggtcagcctt gttgtctcag tgtagatatt gatgccttcg
    23161 cttcggccga ggctccgggt tgcagtcaat cttggacgac agggttgcat attcaagaat
    23221 tcttgcccac tttgcagatg ttacaatccg agctcacggt gaaaagtctt gcgatttacg
    23281 aagtttctcc gcctttggat caagacaatc gcaccagcaa actggccgca ttgattttgc
    23341 accatacgat ttttcacgtt taaaaatttc aggagacttt atgaaaagag gcttcggttc
    23401 cgacaatcat tccggagttc atcctcgcct ccttgaggcc atcaccaagg ccaacgttca
    23461 gcacgcccca agttatggta ctgacgaatg gaccgccaag gccgaaaaag cttttcgtga
    23521 acacttcggt cctaaaactg aaatattttt tgtcttcaat ggcacagccg ccaatgtcct
    23581 ttctttgaaa gcctctctaa aatcatttca agcttgctat tgcgcgagtc ttgcgcacct
    23641 taatgtggat gaatgtggag cccccgaata ccttacggga gccaagctca tcaccgtccc
    23701 cacactgcat gggaagttgc atgtagagga tcttaagcgg gtccacctcc gccgcggaga
    23761 tcaacattcc gttcaaggga aagtggtgag tttgactcaa ccgacagagt tagggactct
    23821 gtactcaatg gaagaactca aggaaatcat cgcctgggcc aaatccgaaa atctttatgt
    23881 gcatatggat ggggcccgcc ttgcaaatgc cgcggtcggc ctacagaaaa catttaaaga
    23941 attcaccacg gatctggggg ttgatgtggt ttcttttgga ggcacaaaaa acggcttgat
    24001 gatgggtgaa gcggttcttt tcttaaaccc cgctctagcc aaagatttca aattctatcg
    24061 taagcaaatg gctcaacttc cttctaaaac ccgctttatt tcagcccagt ttgtggaata
    24121 tctaagcaat gatctgtgga aagaaattgc cacccattcg tgtgaaatgg ctgagaagtt
    24181 atatcaaggg gctaagctcc ttccgggggt ggaggtcaca caaaatcggc aatccaatgc
    24241 ggtctttgcc aaaatccctc aaccttgggt caaagtcttg cgtgaaaagt acttttttta
    24301 tgtttgg
//
LOCUS       DATBTE010000122         3460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_15579, whole genome shotgun sequence.
ACCESSION   DATBTE010000122 DATBTE010000000
VERSION     DATBTE010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15579"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VIG33_04625"
     CDS             <1..467
                     /locus_tag="VIG33_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8270149.1"
                     /translation="VGLDPKQVAEIRELIKELRGQHTIILSTHILSEVQAICDRVIII
                     HQGQIVAQDRIENLAVQDRGQSLLTIRLRKEVEDFKKVLTGIEGIVNVTSGRSRRDWE
                     VNIRGDESVVDAISSRVVTEGLGLLELSPRKIDLEDVFLKLTYGSNQAEVQS"
     gene            464..1252
                     /locus_tag="VIG33_04630"
     CDS             464..1252
                     /locus_tag="VIG33_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8270150.1"
                     /translation="MSAANSATMTIFKKELKGFYMSPGFYLIAFLVSVVLSWSYPIQL
                     NLFSQLLQNYVFQQNLPNQQQLNIHYGLFLRHLSYLNLMLIFVVPALTMRLLAEEKKL
                     RTFDLLLTCPVTSTNIVLGKYFAALAAVCGIVLLALSYPIATATMTTLSWGPLLIAFI
                     GIFLVGATYAAMNLFCSSLTENALVAYVTSVVFNISIWFVGIGVEMTDSQTIRQIFEH
                     ISVNTHLSSLVEGTIRTSALVFLFSVMALFCFLAERVVESCRWR"
     gene            1258..2802
                     /locus_tag="VIG33_04635"
     CDS             1258..2802
                     /locus_tag="VIG33_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEY8270151.1"
                     /translation="MSRFSKILFGVSGVSILCFILTRLLVGAWIPFLWIPLVFFTVCF
                     FGALAQERKNILEFLSMKTTKHGMNMGALIGLVFVGLVVSNFLAVKHYKVWDFSGSQS
                     NTLSNQSIKLVKSLDSELLVRFFYKKGVEGNEENRKSFRELIKKYQDQSSSIKLEFVE
                     VNERPDLAEQYGVTKGSGVIFLEYKGRKNRIEKIEEQEVTNALVKVTREKDKNIYFVV
                     GHGERDLEEAKEANGLNALKVMLENNRYLVKTLALNLNAKIPEDADVVVIVGPTQKFL
                     DYEIQGLEEYLKHGGNLLMALKSKQTQGLEVLLGRVGLEPMNNYVLNLVETAVGKGVQ
                     QGQTLGTVFSTTDEITKVFGKNEATIFRNPMSFKRDKVPEGVTIDDLVKAPENSMSFS
                     SLEIKGEGPQGAFTLATLAKGKFPGGKLDNIDKSFEMVVVGDTDFLGNQLLMQSLNRD
                     LVLNSIASLAKEENLISVAPKENQVTKLNMTDANFYLFVFLFAIPIPLALLGTSLTLW
                     AKRRHA"
     gene            2804..>3460
                     /locus_tag="VIG33_04640"
     CDS             2804..>3460
                     /locus_tag="VIG33_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270152.1"
                     /translation="MKKTTWFAVFVTVLVGATYYFEFYQAGKEEVLKVKESKIISFPG
                     EQINQIEIENKLGKILLKRDTEGWRMLAPLKDWADNQFVEDFVNGLSSEKSLDTIGDE
                     SANASGGVKWSLYGLDQSLNQEFTKVIFTHQQGTSILIQVSAKKNFEGNSFLRRGTEN
                     KVLIGTSQWILRSQKSPLDFRDKRLFRGKIGAVEEITLKSQKDEFQLVNKDNKWINLK
                     N"
BASE COUNT          963 a          635 c          851 g         1011 t
ORIGIN      
        1 ccgtgggcct tgatccaaag caagtggcag agattcgtga gctcatcaaa gaacttcgtg
       61 gacaacacac cattattctc tccactcata ttcttagtga agtccaagcg atctgtgatc
      121 gggtgatcat tattcatcaa ggtcagatcg tagcccaaga tcgcattgag aacctcgcag
      181 tgcaggatcg tggccagagc ctgctgacca ttcgtcttcg caaagaagtg gaggacttca
      241 aaaaagttct taccggcatc gagggaattg taaacgtgac ttcaggccgg agccgtcgtg
      301 actgggaagt aaatatccgt ggcgacgaat ccgtggtgga tgcgatttct agcagagttg
      361 tcaccgaggg cttgggactt ttggaattga gtccacgtaa aattgatttg gaagatgtat
      421 ttttaaaact gacctatggc agtaatcagg cggaggtaca atcatgagtg cggcaaacag
      481 cgcaacaatg acgattttta aaaaagaact taaaggattc tatatgagtc ctggatttta
      541 tctgattgct tttttagttt cagtggtttt aagctggtct tatccgattc aattaaattt
      601 attctcacaa cttttgcaaa attatgtctt tcaacagaat ttgccaaatc agcaacagtt
      661 aaacattcat tatggtcttt ttcttagaca cctttcttac ctaaacttaa tgctcatttt
      721 cgtggtgccg gcattgacga tgcgattatt ggccgaagag aaaaagctgc gtacttttga
      781 tttgttgctc acttgtccag tgacttcgac aaatatcgtg cttggaaagt attttgcggc
      841 actggctgcg gtttgcggaa ttgtgctcct cgcgctttct tatcccattg ccacggcaac
      901 gatgacgact ttgagttggg ggcctttact cattgctttt atcggtattt ttttagtggg
      961 ggccacttat gcggcgatga acttgttttg ctcaagcctc accgagaacg ctttagtagc
     1021 ttatgtaact tcagtggtct ttaatatttc gatttggttt gtaggtattg gagttgaaat
     1081 gacggacagt caaaccattc gtcagatctt cgaacacatt tccgtaaaca ctcatctcag
     1141 tagtttggtg gaagggacca ttcgcacaag tgccctggtg ttcttgttta gtgtgatggc
     1201 tttattctgt ttcttggctg agagagttgt tgaatcttgt cgttggaggt aatttcgatg
     1261 agccgtttta gtaaaatttt atttggcgtt tccggtgtta gtattttgtg ctttattttg
     1321 actcgtcttt tggtaggggc ttggatccct tttctttgga ttcccctcgt cttttttacc
     1381 gtctgcttct ttggagctct ggcgcaagag cgaaaaaata tacttgaatt tctgagtatg
     1441 aaaaccacga agcatgggat gaatatggga gcattgattg gcctcgtatt tgtgggcctg
     1501 gtggtttcca actttttagc cgtgaaacat tataaggttt gggatttttc cggaagtcag
     1561 agcaatactt tatcaaatca atccataaaa ttagtgaaga gtctggactc agagttgcta
     1621 gttcgtttct tttataaaaa aggagtggaa ggcaatgaag aaaatcgcaa atctttccgg
     1681 gaattgatta agaaatatca ggaccaaagc tcttcgatta agctggagtt cgttgaggtc
     1741 aatgaacgcc cggacttggc tgaacaatat ggggtgacca aaggcagcgg cgtgattttc
     1801 ttggagtaca aaggtcgcaa aaaccgcata gagaaaatcg aagaacaaga agtgaccaac
     1861 gccctggtta aggtcacgcg tgaaaaagat aaaaatattt attttgtcgt gggccatggt
     1921 gaaagagatt tggaagaagc caaagaggcc aatggattga atgctttgaa ggttatgctg
     1981 gagaacaatc gctatttagt taaaacttta gctttaaatt taaacgccaa aatcccagaa
     2041 gatgcggatg tggtggtcat tgtagggcca acgcaaaaat ttctagatta tgaaattcaa
     2101 ggtttggaag agtatctcaa acatggcgga aatctgttga tggctttaaa atccaaacaa
     2161 acccaaggcc tggaagttct gttaggacgg gtgggcttgg agcccatgaa taactatgtt
     2221 ctcaacctcg tagagactgc ggtgggaaaa ggggtgcagc agggacagac tttgggaact
     2281 gttttttcca caacggatga gatcaccaaa gtctttggta agaatgaagc caccattttc
     2341 agaaatccca tgtccttcaa acgtgacaaa gtgccagaag gagtgactat cgatgacttg
     2401 gtgaaagcac cggaaaactc gatgtcattt tccagtcttg aaatcaaagg cgaaggaccg
     2461 caaggagcct ttaccttggc aacgctggcg aaaggtaagt ttccgggtgg taagctggat
     2521 aatattgaca agagtttcga aatggttgtg gttggagaca cggacttttt aggcaatcag
     2581 ctcctcatgc agagcttaaa tcgtgatttg gttttaaact ctatcgcttc tttggcaaag
     2641 gaagaaaatc ttatcagcgt ggctccaaag gaaaatcagg tgaccaaact gaatatgaca
     2701 gatgcgaatt tttacttgtt tgtatttctg tttgccatcc ccattccgtt ggccctcttg
     2761 ggaaccagtc tgactttatg ggcaaaaagg agacatgctt aatatgaaga agacgacgtg
     2821 gtttgctgtc tttgttacgg tgttggtagg agccacttat tattttgaat tttaccaagc
     2881 cggaaaagag gaagttctta aggttaaaga gagcaaaatc atttcttttc ctggggagca
     2941 gatcaatcaa atcgaaattg aaaataagct gggaaagatt ctactgaagc gtgataccga
     3001 aggctggcgt atgttggcgc ccctcaagga ttgggccgac aatcagtttg tggaagattt
     3061 cgtcaatggt ctgagcagcg aaaaaagtct agacaccatt ggtgatgaga gtgccaacgc
     3121 gagtgggggt gtgaagtggt ccttatatgg cctcgatcag agtctcaatc aagagtttac
     3181 gaaggtgatt ttcactcatc agcaaggcac ttctattttg atccaagtct ctgcgaagaa
     3241 gaattttgaa gggaatagtt ttctccgccg tggcactgaa aataaggttc tcattgggac
     3301 ttcgcagtgg attctgcgaa gtcagaaatc acccctggat ttccgtgaca aaagactttt
     3361 ccggggaaaa atcggagcgg tcgaggaaat cactctgaaa tctcaaaagg atgagtttca
     3421 gttggtcaat aaggacaata aatggatcaa tttaaaaaat
//
LOCUS       DATBTE010000123        12566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1655259, whole genome shotgun sequence.
ACCESSION   DATBTE010000123 DATBTE010000000
VERSION     DATBTE010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 12566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12566
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655259"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1744)
                     /locus_tag="VIG33_04645"
     CDS             complement(<1..1744)
                     /locus_tag="VIG33_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY8270153.1"
                     /translation="MGLLKGISRLVLTGSFLFFAPLSYAQSKDDLECRYMSAIEGGFL
                     ANHIHYANRDVELANHTIEQYIKRIDPSKVYLLSTDVDQIKGWMAGLFDKTKNKDCAF
                     LDQTQKLLVQRAQERADFAKKFLGKDYKFDPKTEFTFDPDKKAFPKNAEEANDFLKKY
                     IHFQIANYQATDMKMDEAKKNVIKNYDRAVKRLQDTKTTDLYSDYLDSFARALDPHSS
                     FFSRDSLEDFEIQMRLSLEGIGATLSSQDGFTVVEQLVPGGAAAKSGVIEPQDKIIAV
                     GQDKGGMENVIDMDLKDVVKKIRGAKGTKVRLTILRKKGEGKQRLDISLTREKVNLED
                     EAASIIYQDKEVNGKKKKIGIINFPSFYADSRRGGRSSAADLKKILKEANEKKVEGLV
                     LDLSNNGGGSLEDAVKIAGLFFQTGNVVKQSSRDASRGEQTLSDTESTVDWAGPLVLL
                     TSRISASASEIVAGTLQDYKRAVIVGGDHTFGKGSVQSVLPMPNNLGALKVTVGMFFV
                     PGGNSTQHRGVEADVVLPGPYSTDDIGEKFLDYSLPPQKIASFLSPEAYVKEGSGTWS
                     MLKPEWLKSLREKSV"
     gene            complement(1858..2763)
                     /locus_tag="VIG33_04650"
     CDS             complement(1858..2763)
                     /locus_tag="VIG33_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270154.1"
                     /translation="MTGSSQKKQLELGIQRLHENDRNYCLGGRSFYFFDFDDNIAFLS
                     TPLILFHKETGAELRVSSGEYAKIHPFIGRSGPYRNYETRLDDETGTYRNFRDLQFSE
                     LEKIEGKKQFFVKDLAEALGFPDVQWKGPSWQCFYHATFNRRPISVITARGHHPETLK
                     EGIRLLVDGQFLPHEPNYLSLFPVNYKPTRHLLGDKDLSMSAAELKQKAIRASVEKAI
                     ELYGHNSHHRFGMSDDDPKNIQLIAEEMSRLKADYAEMSFFLIATHEGGFVKYEITSQ
                     GIESKVGEGTPEDQLKLFDVDRHKS"
     gene            complement(2794..3414)
                     /locus_tag="VIG33_04655"
     CDS             complement(2794..3414)
                     /locus_tag="VIG33_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270155.1"
                     /translation="MRKSLAALFVFSFFTSSFSYAKWIDPEAEDRKHRQVKAWQGIDF
                     TFTGFSSSSGTTAVTYRNAINDLWEWNAGTGIDSLGWFFTGGGNYFLYNWPHTTCFFT
                     FACHGQVLAGGNLYYANGGRRTYTNTGGDTVYDQGNSIYALPQVAFRSIYREFFSLTL
                     DVGYRVMVQKPSIRRSYGPPAQSNVDDMEKSNVNGLGASVSLGIVF"
     gene            complement(3424..6120)
                     /gene="topA"
                     /locus_tag="VIG33_04660"
     CDS             complement(3424..6120)
                     /gene="topA"
                     /locus_tag="VIG33_04660"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEY8270156.1"
                     /translation="MAKKSTDGIKLVIVESPTKAKTIRKFLGRDYVVESCMGHIRDLP
                     KSAKDIPEKVKKEKWAQLGVNVDKHFEPLYCVPKDKTKVVKNLKDKLEDASELFLATD
                     EDREGESISWHLLQVLKPTVPTKRMVFHEITNDAIQKSLKDTREIDFNLVKAQEARRV
                     LDRLVGYTISPLIWKKVSYGLSAGRVQSVAVRLIVEREHERIRFKTSSYWGILAHLQK
                     DGVDFEARLQNVKSQRIATGKDFDGTTGKLVAGKDVLVLDEIKAKDLVNATKNGLWAV
                     TEVDEKPTTRRPAAPFITSTLQQEANRKMGLSSRETMQVAQKLYEQGFITYMRTDSTF
                     LSTEAITASRNGIQTKYGKEFLPDQPRTYAAKKVKGAQEAHEAIRPAGQQFVDPDETG
                     LVGTQLKLYDMIWKRTIASQMVDARQKQVSAKIQVGDALFGASGMTIEFPGFLRAYVE
                     GSDDPEAELSEREIRLPALKAKDSVQATKVDPTSHETKPPARYTEASLVQTMEKEGIG
                     RPSTYASVIGTIVDRGYVRKHGAALVPTFTAMVVSKLLSRYLPQYVDIGFTSQMEKSL
                     DDIADGDLELEKYLKSIYFGPQGLKEQVAVQADKIDPEEARTIQLEGMDKYNFHVGRY
                     GAYLTSQRDGQDVSASLPESESPADITPEIAERLIDQKINGADALGKDPKTDKPIYVL
                     NGRYGPYVQLGDVSPEDDKPKRASLPPGVQPEDITLPIALELLSLPKTLGTHPGTGKE
                     IKAGLGRFGPFVVCEGDYRSIPKGESIFTMTLERALEMLSTPKKGRGKAAPLKDLGIH
                     PGSEEVIQIFNGPYGPYIKSGKTNVSLPEGATVETVTLEQAIELINDKAGTQTKAKSK
                     AKPKVKEAKVAKTAKKVAAKVGKAEQLKVTKVITRKKSATKK"
     gene            6183..7004
                     /locus_tag="VIG33_04665"
     CDS             6183..7004
                     /locus_tag="VIG33_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEY8270157.1"
                     /translation="METAQVQILTLRFLTSSDYTQIMSKIKFLLTDIDDTLTDEGLLG
                     PEAYSSLWSLHRAGIHVIPITGRPAGWCEMIARQWPVSGVVGENGGFYFRYHNKKMLR
                     HFFFDQKIQKENREKLNHLEKEILTKVPGCALASDQFCRLMDLAIDFCEDVSALPKSE
                     VQKIVEIFEQHGAQAKVSSIHVNGWFGTYDKLTMTLTMLQKEFGTSPEKAKQVCAFSG
                     DSPNDEPMFGYFPHSYAVANIQTFLGDLKQKPTHIAHSRGGLGFAEIAKGILEGN"
     gene            7293..9035
                     /locus_tag="VIG33_04670"
     CDS             7293..9035
                     /locus_tag="VIG33_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270158.1"
                     /translation="MKSVRIWLLMISFLILHEYVFADSSASCGMANCSSYKSPDVSAK
                     IAACELTLATHDCESLGSDIQLRDCKTEAYCSNFLDNSYIAGCVFGVKDFAVGIITSP
                     IELARQIRKQLDYDSRYSNSYLYRSCKQAEDKVNSGRLSAQELDCQQTFWRSACPRTI
                     LNSCKESLLLEFPDIKASYGSGYSQISYDQVYKDTHKRLNAINSTKPSLAKFLEEHRE
                     HPLDDIANIVRESLRKESACLSPKSFSKISCDRILNIVSLLSGGYAAIKKIGKTAAIS
                     SELTSPSTLSSKLERMGLKNSHNGNPTVRLPRKGKYPENIQLSQDSIYFEDAVIKPGP
                     DASLNAIIAEVERAGGEVRSIDKLSLGVLPSTKVENLSYFIIDKSGKPIIALEKGADA
                     ATIAHELDHFRMWQSFKEKFIKEGLSEKEAIAKANKVMTSSSPKLIRSAEASAVDAEL
                     QHRNFSLFNSDLTKRIVYPERAAITKTYEQATDIKKIPKKYLAQVYQQMDAAIAKALT
                     IRELRISEIQIQIRKPNITPSEYNQLNIKLTEQFNYDTLGKEMGFIDSSKSMKLFKTR
                     FPEVLKRQSSSNSN"
     gene            9582..11336
                     /locus_tag="VIG33_04675"
     CDS             9582..11336
                     /locus_tag="VIG33_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEY8270159.1"
                     /translation="MIRKYLVLFLVTPVMMNIGCQPKPSPTAGRPPSPVSLAFEKVEQ
                     TCLKSLKAHMLKGDFSFREVSNPQINAEIKNVMATLMIAGADIEYRLSHDVKTNLTTS
                     IEVNHSKKKEQGDVAKYEMRLSVPDKDSEYLFALNLTYSEDAASGINTSLNQRYRIDS
                     NCRPLLIETEYTKTTLANLKISAIHLEEFADGKAGSFHSSQGETSIPSDGILMTAESS
                     SDLKTWESLNTFLSLQKKDAIYYSRTQFAPYFIQSKIDFAPDTEHFDPILGRQAKCHQ
                     LIIHIEGMGDILSLFSTNSNFTTSRFGNGDENWTVNSALFESATLSRSASNSNRTFEI
                     SSELLAGTQSLRAEFLTSRELKYPNLESYWKVEHKEQVPEPADGLPFKYTLLSQKITA
                     QDQDSDNLIIDPKEKKQNPDLQESANLQISIAPVQAMVAKLQSNGPLNRLQMARLIGK
                     MVTETLTYDYDSVKESSIHALTTTEVLERKKGVCQHFANLFAVLARGVGIPARFITGY
                     YLSPQGVGAHAWNEIEIRSGVWMPIEPQNSNLSLNPMFYFPLVTGGYMETMKDEDAFR
                     DGKAMLIEFHAKALESIR"
     gene            11333..12301
                     /locus_tag="VIG33_04680"
     CDS             11333..12301
                     /locus_tag="VIG33_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270160.1"
                     /translation="MIHKSLALSLIFPLMVNVGCQPNSSPIAGRPQPPASATLKKVEQ
                     SCLRTLKTRMVKSDFSFVEVSNPQLNNEVKNIMTLPLMPTPEIEYTLTQGIQKNSFTS
                     MGVSHSKKANGNVSRQEISLSVPEKDVGYLYTLNLTYSIEAAASGTNTSLNQKFKIDS
                     NCHSSLAETDFTRTTLSDFKISALHLEESAEEKMDPFHSSRAQTEVPTDGVLWSVEPL
                     NEKLATWKDLSAFLSSQEKNVLYFSRIQEAPYILKMRIDLAPDVEHFDPLVGHQAQFH
                     QLAVHFKRFGDSILFYSTNSEFRFRRFDNGTEKWIVNSALFENASF"
BASE COUNT         3616 a         2890 c         2487 g         3573 t
ORIGIN      
        1 ccacggattt ttcacgcaag ctttttaacc actctggttt caacattgac caggtaccag
       61 agccttcttt cacgtaagct tcaggagaca aaaatgatgc gattttttga ggtggcagag
      121 agtaatccaa aaacttctcg ccaatatcgt cagtgctata aggacctggc aacaccacat
      181 cggcttcaac accacgatgt tgggtggagt tcccccctgg gacaaagaac attccaacgg
      241 ttactttcaa agcacccaag ttattcggca taggcaatac ggattgaacg ctgcctttac
      301 caaaagtatg gtcaccgcca acaatcacag cacgtttata atcttgcaga gttcccgcca
      361 cgatttccga agctgaagca ctgatgcgac tggtaagaag aaccaggggt cctgcccagt
      421 caaccgtgga ttccgtatcg ctcaaggttt gctctccgcg acttgcgtct ctagaagact
      481 gtttcaccac attgcctgtt tggaaaaaca aaccagcgat tttcacagca tcttccaagc
      541 taccaccacc gttattagat aagtctaaaa cgaggccttc tacttttttc tcgttcgctt
      601 ctttgaggat ctttttcaag tccgccgctg aagaacgtcc gccccgccga gagtccgcat
      661 agaaagaggg gaagttgata atcccgatct ttttcttttt accattcact tctttgtctt
      721 gatagatgat agaagccgct tcatcttcca ggtttacctt ctcacgagtc aaactgatat
      781 ccaaacgttg tttgccttcg ccttttttac gcagaattgt taaacgaact ttagtccctt
      841 tggcgccgcg gatttttttc acgacatctt ttaagtccat gtcgatgaca ttttccatac
      901 cccctttgtc ttgtccaaca gcgatgattt tatcctgtgg ttcaatcact ccagattttg
      961 ccgccgctcc gcccggaacc aattgttcaa caacagtgaa cccatcttga gaagaaagag
     1021 tggcgccaat accttctaaa gacaaacgca tttgaatttc aaagtcctcc agactgtcgc
     1081 gagaaaagaa gctggaatga ggatccaaag cccgtgcaaa actatctaaa taatccgagt
     1141 acaaatccgt cgtcttggta tcctgcaggc gttttacagc tcgatcataa ttcttgatta
     1201 cgttcttctt cgcctcgtcc attttcatat cggttgcttg gtaattcgca atttggaaat
     1261 ggatatactt cttcaagaag tcattagctt cttcggcatt tttaggaaac gcctttttgt
     1321 ctggatcaaa agtaaattca gtttttggat caaatttata gtcttttcct aaaaactttt
     1381 tcgcaaagtc cgctctctct tgagcgcgct ggaccaacaa tttttgagtt tgatccaaaa
     1441 atgcgcagtc cttgtttttc gttttatcaa agagtcccgc catccagcct ttgatttgat
     1501 cgacgtctgt ggaaagaaga tatactttag acggatcaat acgtttgatg tattgctcga
     1561 ttgtatgatt cgctaactcc acatcccggt tggcatagtg gatatggttt gctaaaaaac
     1621 ccccttcaat cgcactcata tatcgacact caagatcatc tttactttga gcataagaga
     1681 ggggagcgaa aaacaaaaaa gatcctgtca atacgagtcg ggaaattccc ttgagtaaac
     1741 ccatgtgtat ccttcctagt gatctatcga aatttcaatt tccttagaat aacaaatatc
     1801 taagcgagat catttaaatt atcggctaat taaagaaaaa gaatagtcgg ctatgcgtta
     1861 agacttatgt ctatccacat caaagagttt aagttgatcc tcaggagtgc cttcccctac
     1921 tttagactca atcccttggc tcgtgatttc atatttcaca aaaccgcctt catgggtggc
     1981 aatcaagaaa aaggacattt cagcatagtc tgcctttaag cgagacattt cctcagcaat
     2041 taattgtata ttcttcgggt catcatccga catcccaaac cgatgatggg aattatggcc
     2101 ataaagttca atagcttttt caacgctggc tctaatggct ttttgtttaa gttccgccgc
     2161 gctcatgctc aaatctttat ctccaagcag atggcgagtg ggtttgtagt tcacaggaaa
     2221 caaactaaga taattgggtt catgaggcaa aaattgtccg tcgactagaa gacgaattcc
     2281 ctctttaaga gtctcaggat gatggcctct ggctgtaatc actgaaatgg ggcggcgatt
     2341 aaatgtggcg tggtaaaaac actgccatga gggccccttc cattgaacat ccggaaagcc
     2401 caaagcttcc gccaaatcct ttacaaagaa ttgttttttt ccctcgattt tttcgagctc
     2461 actgaattgc aaatcacgaa aattccgata ggttccagtc tcatcatcaa ggcgggtttc
     2521 gtaatttcga taaggtcctg aacgaccgat aaagggatgg attttcgcat attctccgct
     2581 gctgactcgg agttcggctc cagtttcctt atgaaacaga atcaagggag tggataaaaa
     2641 agcgatatta tcatcgaaat caaaaaagta aaaactgcgg ccaccaagac aatagtttcg
     2701 atcgttttcg tgaagccttt gaattcccaa ttccaattgt tttttttggg aactgcctgt
     2761 catacaaaaa cctcaactcc aaccccataa tctttaaaat acaataccca gcgaaactga
     2821 ggctcccaaa ccatttacat tggacttttc catatcatcc acattgcttt gggcaggagg
     2881 accataactt cgacgaatgc tgggcttttg taccataact cgatatccca catctagtgt
     2941 gagtgaaaaa aactctcggt aaatcgatcg aaatgcgacc tgaggaagag cataaataga
     3001 gtttccttga tcatagacag tgtctccccc cgtatttgta taggtccggc gcccgccatt
     3061 tgcataatat aaatttcctc cagctaagac ttgaccatgg caagcaaagg taaaaaagca
     3121 agtggtatga ggccagttat atagaaaata gtttcctccc ccagtaaaga accaacctag
     3181 cgaatcgatg cctgtaccgg cattccattc ccagaggtca ttgatagcat tccgataagt
     3241 gacagcagtc gttccagagg aagaagaaaa ccccgtaaaa gtgaaatcga ttccctgcca
     3301 agcttttacc tggcgatgct tcctatcttc tgcctcagga tcaatccatt tagcataact
     3361 gaaggaactt gtaaaaaatg aaaaaacaaa tagagctgct agagactttc tcattcctct
     3421 tccttatttt ttagttgctg acttcttgcg ggtaataact ttggttactt tcaactgctc
     3481 ggcttttccc accttggcag ctactttttt cgctgttttt gcgactttag cctcttttac
     3541 ttttggcttc gctttacttt tcgccttagt ctgagtccct gctttatcgt taatcaactc
     3601 aatagcttgt tccaaggtaa cggtttcaac cgtagctcct tctggtaagg acacattcgt
     3661 tttacctgac ttgatatagg gaccataagg accattgaag atttgaataa cttcttcact
     3721 accggggtga attcctaaat ccttaagagg agctgctttg ccacgaccct ttttaggagt
     3781 actcaacatt tccagagctc tttccaaggt catcgtaaag atactttcac ctttaggaat
     3841 agatcgatag tctccttcac aaaccacaaa aggaccaaag cgacctagac ccgctttaat
     3901 ttctttacca gtccccggat gcgtccccaa cgtcttcgga aggctcaaaa gctccaaggc
     3961 gatcggcaaa gtaatatctt caggctgaac ccctggaggt aaagaagccc gctttggttt
     4021 atcatcctca ggactcacat ccccgagctg aacatagggg ccatagcgac cattcaaaac
     4081 ataaatcggc ttatctgttt taggatcttt gcctaaagca tcggcgccgt tgatcttctg
     4141 atcaataagc ctttccgcaa tttccggagt aatatccgcc ggcgattcac tttcagggag
     4201 cgatgcactc acatcttgac cgtcgcgctg tgaagttaaa taagctccat agcgacctac
     4261 atggaaattg tatttatcca tgccttccaa ttgaatcgtc cgagcttctt caggatcaat
     4321 tttatcggcc tggaccgcaa cttgttcctt gaggccttgg ggaccgaaat aaatcgattt
     4381 caaatacttt tcgagttcta aatctccgtc agcgatatca tccaaagatt tttccatttg
     4441 cgacgtgaag ccaatatcca cgtactgggg caaataccga ctcaagagtt tcgaaaccac
     4501 cattgccgtg aaagtcggaa ccaatgcagc tccgtgtttt cttacatagc cccggtccac
     4561 gatcgttccg ataaccgaag cataagtcga aggacgacca atgccttctt tctccatggt
     4621 ttgaacaaga ctggcctctg tataacgtgc cggaggtttc gtttcatgag aggttggatc
     4681 tacttttgtc gcctgcacgc tgtctttggc tttgagtgcg ggaagacgaa tttcacgttc
     4741 tgacaattct gcttcaggat catcgctccc ttcaacataa gcacgcaaaa atcctggaaa
     4801 ctcaattgtc atacctgagg ccccaaagag tgcatcgccg acctgaatct tcgcactgac
     4861 ttgtttttga cgagcgtcga ccatttgact cgcgatcgtg cgcttccaga tcatatcata
     4921 aagtttaagc tgcgtcccca ccaggcctgt ttcatcagga tcgacaaact gctgtcctgc
     4981 ggggcgaatg gcttcatggg cttcttgcgc cccttttact tttttcgccg cataagtccg
     5041 cggctgatcc ggcaaaaact ctttaccgta tttcgtctgg ataccgttgc gagaagccgt
     5101 gatagcttcc gtcgataaga aggtcgaatc cgttctcatg taagtgataa aaccttgctc
     5161 gtacaacttc tgcgccactt gcatggtttc tcgggaactc agacccatct tacgattggc
     5221 ctcctgttgc aaagtggaag tgataaacgg agctgctggc cgacgagttg tgggcttctc
     5281 atcgacttcc gtcaccgccc aaagaccatt cttcgtggcg ttgacaaggt cttttgcttt
     5341 aatctcatca agaactaaaa cgtctttacc agccacaagc ttccctgtgg ttccatcaaa
     5401 atctttacct gtcgctatac gctgactttt aacattttgc aggcgagcct caaaatccac
     5461 accatctttt tgcaaatggg caaggattcc ccaataactt gaagtcttaa agcgaattcg
     5521 ctcgtgctct cgctctacga tcaaccgcac cgccaccgat tgaacccggc cggctgataa
     5581 accataggaa acttttttcc aaataagagg cgagattgtg taacctacca aacgatccaa
     5641 aactcgacgc gcctcttgcg ctttcaccaa gttaaaatca atctcacgag tgtctttcaa
     5701 agatttttgg atcgcatcat ttgtgatctc atggaacacc atgcgctttg tcggcacggt
     5761 gggctttaaa acttgtagca aatgccaact gatactttcc ccttcacggt cttcgtccgt
     5821 cgcgaggaaa agctctgagg catcttcgag tttatctttc agattcttaa ccactttggt
     5881 cttatctttc ggaacacagt aaagaggctc gaagtgttta tcgacgttca ccccgagttg
     5941 cgcccacttt tcttttttca ccttttcagg gatatcctta gctgacttag gcaagtcacg
     6001 aatatgcccc atgcaggatt ccaccacgta atctcttccg agaaatttac gaatggtttt
     6061 cgccttggtc ggagattcca ctattactaa tttaataccg tctgttgatt tcttcgccat
     6121 tttttatacc ttcatcatga tgtgcaggca ctactaatat atagacaaac catgaggtgg
     6181 atttggaaac tgcgcaagta caaattttga ctttgcgctt cctaactagc tccgactata
     6241 ctcaaatcat gtcgaaaatt aaatttttac tcactgatat cgatgacact ctcacggacg
     6301 aaggactctt gggtccagaa gcctactcct ctttatggag tttacatcgc gccggaattc
     6361 acgtgattcc cattactgga cgtccagccg gttggtgcga aatgatcgct cgtcagtggc
     6421 ccgtcagtgg cgtcgtcggc gaaaatggcg gattttattt ccgttatcac aacaaaaaaa
     6481 tgctacgcca tttttttttc gatcaaaaaa tacaaaaaga aaaccgcgaa aagctcaatc
     6541 atctcgaaaa agaaatcctt accaaagttc cgggctgtgc tttagccagc gatcaatttt
     6601 gccgcttaat ggatttagcc atcgatttct gtgaagacgt ctcagccctc ccaaaatccg
     6661 aagtgcaaaa aatcgtggag atctttgaac aacatggagc tcaagccaaa gtttcttcta
     6721 tccacgtgaa cggctggttt ggcacttacg acaaactcac gatgacgttg acgatgctgc
     6781 aaaaagaatt cggcacaagc cctgaaaagg ccaagcaagt ctgcgccttt agcggtgact
     6841 ctcccaatga tgagcctatg ttcggctatt ttccgcactc ttacgctgtt gccaatattc
     6901 aaactttctt aggggacctg aagcagaagc ccacgcatat cgctcactct cgaggcggcc
     6961 ttggctttgc ggaaattgct aaggggatct tggaaggcaa ctgatctggt tgcttgcact
     7021 gggtctgatt gccggtgatc tagcgaggag ccattcgcaa cccagaacca ttgggattta
     7081 gatggggtct gacagtgtca acacggctca tttcaagtcc ttttctgctc gaatataccg
     7141 tgggtttaga agccattggg tttcgataaa gattttggat aagtccagaa ttagatagag
     7201 gatgccagtc ataggtccac aagctaattg tattgctcta taggcacaag atccagtcaa
     7261 gctggcttgt ctagtttccg aaaagtaaag agatgaaaag tgtcagaatt tggttgctca
     7321 tgatcagttt tttgatttta catgaatatg tctttgcaga ttcatcggca tcctgtggga
     7381 tggctaattg ttcctcttat aagtcccctg acgtctcagc taaaattgca gcttgcgagc
     7441 taacattagc aactcatgat tgtgaatctt taggatcaga cattcaatta agagactgta
     7501 aaactgaagc ttactgttct aattttttgg ataatagcta tattgccgga tgtgtatttg
     7561 gcgtaaagga ctttgcagtt ggtattatta cttcacccat tgaactggcg agacaaataa
     7621 gaaaacaact ggattatgat tcgagatatt cgaattctta tctttatcgt agttgtaagc
     7681 aagctgagga taaagtaaat agtggcaggt tgtctgcgca agaacttgat tgtcaacaga
     7741 ctttttggcg atcagcttgt ccaagaacaa ttctaaatag ctgcaaggaa agtttacttt
     7801 tagaatttcc tgatatcaaa gcttcttatg gttctggata ctcgcaaata tcctacgatc
     7861 aagtttataa agatacacac aaaagattaa atgcaattaa ttcaacaaag ccctcgctag
     7921 ctaagtttct agaagagcat agagaacatc ccttagatga tattgcaaat attgtcaggg
     7981 aaagtttaag aaaagaatct gcttgcttaa gtccaaaaag tttttcaaaa atttcctgtg
     8041 atcgaatcct aaatatagtt tctttgcttt ctggaggata tgcagctatt aaaaaaatag
     8101 gaaagactgc tgctatttct tcagaactga catctccttc cacactatcg agtaaacttg
     8161 agcgaatggg attaaagaat agtcataatg gcaatcctac agttagattg cctcgaaagg
     8221 gcaagtatcc agaaaacatt caactcagcc aagattcaat ttactttgaa gatgcagtga
     8281 taaaaccagg ccctgatgca tcactaaatg cgattatagc agaggttgaa cgtgcaggtg
     8341 gagaagttag aagtatagat aaactttcac ttggggtttt accaagcaca aaagtggaaa
     8401 acctatctta ctttattatt gataaaagtg gtaagcccat aattgctctt gaaaaaggag
     8461 ctgatgctgc gaccatcgcg cacgagttag atcattttcg catgtggcaa agctttaaag
     8521 aaaaatttat taaagaaggg ctttctgaga aagaagctat agccaaagcg aataaagtga
     8581 tgacatcttc cagccctaaa ctaataagaa gcgccgaagc tagcgctgta gacgcagaac
     8641 tgcaacatag aaatttttct ttatttaatt cggacttaac caagcgtatt gtatatcctg
     8701 aaagagctgc cattaccaaa acatatgaac aagcgactga tattaaaaaa attcctaaaa
     8761 aatatttagc gcaagtttat cagcaaatgg atgcagctat tgcaaaggca ttaaccatca
     8821 gggaattacg aatttcagaa atacaaattc aaataagaaa gcccaatatc acgccttcag
     8881 aatataacca gcttaatatt aagttaactg aacaatttaa ttacgatact cttgggaaag
     8941 aaatgggttt cattgattct tcaaaatcaa tgaaactctt taaaactcga ttccctgaag
     9001 tgttgaaaag acaatcatca agtaattcta actaatcgcc catgaccata ttaagcgaat
     9061 tcaattccgg aaattccaga aaacggattt attttgtctg aactctgcct taaactgaat
     9121 ctaaggatgg tcactgcttt gcaaacaaga aaagcggccc tgaaagccgc ttttaaggat
     9181 gagagactcc ctttttcaaa ggaggatccc atgaagaaat atcaccacct gatatctctt
     9241 cggctatctg gaaaaaatct gcaggtcttg ctcttcattt tgttgttact gttgagctgc
     9301 tgcagttagg ttttttgata ggttctatta agggcttggt actaccaaag tcctggtaag
     9361 tgagcgacca ggctataaac ttggagttgc gagcaaggac gaagctttcg gggccacctt
     9421 ttttattcag gacaaccatc tcggtaactg gccgcacagc tcaggtatgc ccaaaagttc
     9481 gacaggtaaa atttggatgt ccaaatcatt acagattaat tttaattttc aatctgctac
     9541 tctaggccgc cattgaaaaa gcctgcactg tggagaattt aatgattcgc aaatatttag
     9601 ttttatttct tgttactccg gtgatgatga atattggatg ccaaccaaag ccttcgccga
     9661 ctgcgggtcg tcccccctct cccgttagct tagcttttga aaaagttgag cagacatgtt
     9721 taaagagcct aaaagcccat atgctaaagg gcgacttttc atttcgtgaa gtttcgaatc
     9781 ctcaaataaa tgcggaaatt aaaaatgtca tggcaactct catgatagcg ggcgctgata
     9841 tagaatacag actaagccat gatgtaaaaa ctaacttaac cacctccatc gaggtcaatc
     9901 attccaagaa gaaggaacag ggggatgtcg ccaaatatga gatgcgtcta tctgttcctg
     9961 ataaagactc tgaatattta tttgcactga atttgactta ttcggaagat gcagcttcag
    10021 gaattaatac aagtttaaat caaaggtatc ggatcgattc aaattgccgt ccccttctca
    10081 ttgaaactga atatacaaaa acgacattgg caaatttaaa aatttcggcg attcatcttg
    10141 aggaatttgc agatggtaaa gcgggctctt tccattcttc gcaaggagaa acttctattc
    10201 cgtccgacgg aattctaatg accgccgaat ctagctcaga cctaaaaacc tgggaaagtc
    10261 ttaacacatt cctctctttg caaaaaaaag acgctatcta ctactcaaga acgcaattcg
    10321 ctccttactt tatacaatcg aagattgatt tcgctccgga tactgagcac ttcgatccaa
    10381 tactaggccg acaagcgaaa tgccatcaat tgattattca tattgaggga atgggagata
    10441 ttttatcctt gttctcaaca aattcaaatt tcaccacctc tcgctttggc aatggtgatg
    10501 aaaattggac cgttaactct gccttatttg aaagcgcaac tctctctcgc tcagcaagca
    10561 attccaacag aacattcgag atcagttctg agcttcttgc gggaacacag tctctccgag
    10621 ctgagtttct tactagccgt gagttgaaat atccaaatct tgaaagctat tggaaggtgg
    10681 aacacaaaga gcaagtccct gagccagccg atggtctccc tttcaaatat actttactaa
    10741 gccaaaaaat cacggctcaa gatcaagatt cagacaatct gataattgat ccaaaagaga
    10801 aaaaacaaaa tcccgattta caagaatcag caaacttgca aatttcaatt gcaccagtgc
    10861 aagctatggt cgcaaaattg caaagcaatg gccccctcaa cagattgcag atggcccgcc
    10921 ttattgggaa aatggttaca gaaaccctaa cttatgatta cgattctgtt aaagagtcca
    10981 gcattcatgc actgactacg acggaggtcc ttgagcgcaa aaaaggcgtt tgccaacact
    11041 ttgcaaattt atttgctgtg cttgctcgcg gggtcggcat accggcacgg tttattaccg
    11101 gatactactt atcgcctcaa ggcgtgggcg cccatgcttg gaacgaaatt gaaatccgct
    11161 ctggcgtttg gatgcccatc gagccgcaaa attccaacct gagtctgaat ccaatgttct
    11221 attttcccct agtcactggc ggctatatgg aaacaatgaa agacgaagac gcatttcgag
    11281 acggaaaggc catgctgata gagttccatg caaaggcttt ggagagcata cgatgataca
    11341 caaatcttta gctttatctc ttattttccc gctcatggta aatgtggggt gccaaccaaa
    11401 ttcttcccct atcgctggcc gacctcagcc tccggccagc gcgactttaa aaaaagttga
    11461 acagagctgt ttaaggactc tgaagacccg tatggttaag agcgactttt catttgtcga
    11521 ggtctcaaat cctcaactga ataatgaagt taaaaatatc atgaccctac cgttgatgcc
    11581 gacccccgaa atcgaataca cactcaccca aggtatacaa aaaaactctt ttacttccat
    11641 gggagtcagc cattcaaaaa aagctaatgg caatgtctcc agacaggaga taagtctttc
    11701 cgttccagaa aaggatgttg gatatctata tacactcaat ttaacctatt cgatagaggc
    11761 tgctgcttcc ggcaccaaca caagcttgaa ccaaaaattc aagatcgatt caaattgtca
    11821 ctcttccctt gctgaaaccg actttaccag aacgacttta tcggatttta aaatttcagc
    11881 acttcatcta gaggaatccg cggaggagaa aatggaccca ttccattctt cacgagctca
    11941 aactgaggtc ccgaccgacg gcgttctctg gagcgtagag cctctgaacg aaaagttagc
    12001 aacatggaaa gatctcagtg catttctttc ttcacaagaa aaaaatgttc tttacttctc
    12061 cagaatccaa gaagcaccct atatcttaaa aatgagaata gacctagcac cagatgtcga
    12121 acactttgat cctttggttg gacatcaggc tcaatttcat caattggctg tgcactttaa
    12181 aagatttggc gactccattt tattctactc tacaaattct gaattcagat ttcgccgttt
    12241 cgataatggc acagaaaagt ggatagtaaa ttctgcgttg tttgagaatg caagtttctg
    12301 atatccctat tccagaaatt tgttgattta ttttcgatga ccgacttgaa cgaactgccc
    12361 ctccgggaaa cggatttatt tttcctaaat taccctcagc tagctctaac attggacact
    12421 gctttgcaaa cagtaaaagc ggccctgaaa gccgctttta aggatgagag gctccctttc
    12481 acaaaggagg ctcccatgaa aagatatcaa cacgtgatat ctcttcggct atctggaaaa
    12541 aatctgcagg tcttgctctt catttt
//
LOCUS       DATBTE010000124         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_562282, whole genome shotgun sequence.
ACCESSION   DATBTE010000124 DATBTE010000000
VERSION     DATBTE010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562282"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1501)
                     /locus_tag="VIG33_04685"
     CDS             complement(<1..>1501)
                     /locus_tag="VIG33_04685"
                     /inference="COORDINATES: protein motif:HMM:NF039196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY8270161.1"
                     /translation="VAREVGTEGKLGGQAADVEGVSGTWKDLTENVNVMGSNLTVQLR
                     DVSKVATAIANGDLTQKITVEVRGEILQIKDVINKMVDQLNSFGAEVTRVAKEVGTEG
                     KLGGQAEVRGVSGTWKDLTDNVNGLANNLTAQVRNIAKVTTAVAKGDLSQKITVDAKG
                     EILELKNTINVMVDQLNSFAAEVTRVAKDVGTEGKLGGQAEVKGVSGTWKDLTDNVNG
                     LASNLTSQVRNIAKVTTAVANGDLSQKITVDAKGEIFELKNTINVMVDQLNSFAAEVT
                     RVAKEVGTEGKLGGQAEVKGVSGTWKDLTDNVNFMASNLTTQVRGIVKVVTAVANGDL
                     NQKFVLEAKGEVAALAETINSMTDTLRTFADQVTTVAREVGIEGKLGGQAKVPGAAGT
                     WKDLTDNVNQLAGNLTSQVRAIGEVSTAVTMGDLTRSIAVEAQGEVAALKNNINQMIS
                     NLKDTTEKNNEQDWLKTNLAKFSGMMQGQRSITSVAQLIMSELTPLVGAS"
BASE COUNT          316 a          448 c          281 g          456 t
ORIGIN      
        1 acttgcacca accaaaggcg tcaattctga catgatcaac tgcgccaccg acgtaatgct
       61 tcgctgtccc tgcatcatgc ctgagaattt tgcaaggttg gtttttagcc agtcttgctc
      121 attatttttc tcagtagtgt cttttaaatt cgagatcatc tgattgatgt tatttttaag
      181 tgccgctact tcaccttgag cttcgaccgc gatggacctg gtcaaatctc ccattgtcac
      241 tgccgtcgaa acttctccaa tagctcgaac ctgagatgtc aaatttcctg cgagctgatt
      301 cacattgtcc gtcaagtctt tccaggttcc agcggcccct ggaacctttg cttgtccacc
      361 caatttccct tcaatgccca cctctcgagc gaccgtggta acctggtcag caaatgttcg
      421 caaagtatcg gtcattgagt tgatggtctc agccagagcc gccacttccc ctttggcttc
      481 aagaacgaat ttttggttta gatccccgtt ggccaccgcc gtcacaactt ttacgattcc
      541 tcgcacctga gtggttaagt ttgacgccat gaagttcaca ttgtctgtta agtctttcca
      601 agttcctgaa actcccttca cttcggcctg gccacccaac ttcccttcag ttcctacctc
      661 ttttgcgacc cgggtcactt cggctgcgaa ggagttcagc tgatccacca tgacgttaat
      721 ggtgtttttt aactcaaaga tttcaccctt ggcatccact gtgatctttt gcgacaagtc
      781 tccattggcc accgccgttg tcaccttcgc gatattccga acctgagacg ttaggttact
      841 ggctagacca ttcacattat ccgtcaaatc cttccaagtt cccgaaacac ctttcacttc
      901 ggcctgacca cctaattttc cttcagtccc cacgtccttt gccacccgag tcacctctgc
      961 cgcaaaggag ttcaattgat ccaccatgac gttgatcgta ttttttaact ccaggatttc
     1021 acccttagca tccaccgtga tcttttggga caaatcccct ttggccaccg ccgttgttac
     1081 tttggcgata tttcgaacct gggctgtcaa attattcgca agaccattca cattgtccgt
     1141 caaatctttc caagttcctg aaacgcctct cacttcagct tgtccgccca atttcccttc
     1201 tgttccaacc tctttcgcta cccgggtcac ctcggcgcca aaggagttca gctgatccac
     1261 cattttatta attacatctt taatttggag gatttctcca cgcacttcca ccgtgatctt
     1321 ttgagtcaaa tcaccatttg cgatggcggt tgcaaccttt gaaacgtcac gaagttgaac
     1381 tgtcagatta gatcccatga catttacgtt ttcggtgagg tctttccatg ttccagacac
     1441 gccttcaacg tcagccgctt gcccaccaag tttcccttca gtgcctactt cgcgggccac
     1501 g
//
LOCUS       DATBTE010000125         9479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1519391, whole genome shotgun sequence.
ACCESSION   DATBTE010000125 DATBTE010000000
VERSION     DATBTE010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 9479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9479
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519391"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1858)
                     /locus_tag="VIG33_04690"
     CDS             complement(<1..1858)
                     /locus_tag="VIG33_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY8270162.1"
                     /translation="MALNPGCNLIAVVGGKGGVGKSVFAANFAFALMAELRTQVLLID
                     GDSKSVGDQNVILGLKPNKTLKELASMTSSVNAQNMNSLLTMHASGLAYLGAVRGPEE
                     TLNIGPDLAMKQLEFFSRQFKFIIVDIGTDFTPLQRAILQESTAIMIVTTPEILVVTQ
                     TLRLINELLSNTFPKDMFQLIINKASANGLSPQAIATQLQLAPLAIIPQDEITAMASI
                     QKFQPFVIGQPKAAITSAYYEAVRRLSGGVLQKLKSLSRPKPSSAPTAEGNTSAPSSG
                     TASGYDSRTQLKLRIHSELIRTVDLKKLLLDTKGDENKEKEVREKTRREITLITDREA
                     PEQARDERSRIIKEVLEEALGLGPLEDLLADPNVTEIMVNGHKKIFIEKLGKITMSPV
                     TFTSNDHLRRIIERIVSPLGRQINESTPYVDARLKDGSRVNAVIEPLAIDGPALTIRK
                     FKKGGITPEKYTEYGSITKPMIDFLRICVENGLNVVISGGTGSGKTSLLNMLSSFIPS
                     NERVITVEDAAELQLQQEHVVRLETRPPSMEGSNAVTIRDLIKNALRMRPDRIIVGEC
                     RDGAALDMLQAMNTGHDGSMTTTHANSPRECLSRLETLCLMSGMDLPIRAIRE"
     gene            complement(1932..3431)
                     /locus_tag="VIG33_04695"
     CDS             complement(1932..3431)
                     /locus_tag="VIG33_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEY8270163.1"
                     /translation="MKCKYLLGLFLVLSCNYAFAEDDEQAEETKFRSRTFVNLTLGIE
                     QDEKLPPLPENIEFKGDFRRIVTAAHSKELNVLRMTPKAEGFATMTIHDKKTGKVVAE
                     FRIDVKKSKLDKVVKEIRALLGDIEGITIKVVNNKVVVDGQILLPRDLSRIYNVIQQF
                     GDQASSLVTLSPLAQKKIAEFISRDINNPEIEVRAVNDKFILQGYANNEEEYKRAEII
                     AKTYVPDIVIEAAEEKGVIKKRKPANDGVINLITIKEQAPAPPSKMIQLVVHYVELNK
                     DYSKAFKFQFTPQLDDGSQLQFQTGSSGAGGVVSTLTGVVSNLLPKLTWAKEHGHARI
                     LESTSLIIQDGKKGEIKQLTNMPYSVIGKDGQPGTAFAEVGIKSAVTPVILGEKSGSV
                     HMDMDFALSNLLGSGQNGAPITSSNSIQSTVVIRDRQSAAIGGLIRNASSTGYNRMPS
                     DVKNPIISLYASKNFQRNQSQFVVFVTPVIKTSASSGAEQIKKKFRLRD"
     gene            complement(3482..4375)
                     /gene="cpaB"
                     /locus_tag="VIG33_04700"
     CDS             complement(3482..4375)
                     /gene="cpaB"
                     /locus_tag="VIG33_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY8270164.1"
                     /translation="MGSNETRNLWVSIGAGAIAAILLYSWSQEKRAEYNKKYGSLKTV
                     VVAKEDIAEMRTVYDTMLTTIDRPSEFVDADAVSNPDEIVGNVAAVPIKKGQIIVKNS
                     LLTPGPDTGISLQIAPSKRALTIPVDEVRGVAKLIRPGDRIDILAAVDTGKGINQKRE
                     VQTLLQDVVVLATGVNIMNNIPRVFELDSSGKSLSQIALTGDTKYSTITIEATQKEAQ
                     DLVYILSTAPGNIYLTLRNPNDRTIPPRLPTSSAESVLGKPMISMDAMPTAPVAPPVM
                     MAPAPRTAPTLPQKKHGFKTL"
     gene            complement(4388..4879)
                     /locus_tag="VIG33_04705"
     CDS             complement(4388..4879)
                     /locus_tag="VIG33_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270165.1"
                     /translation="MLTPARQLRNNQGMAVMEIIPIMIIIVILFNFSLGFFGAIHTGI
                     LNSMAARNYAFETFRHRSNLVYLRDGVSAEDNVSFGSQNMRIHATVSSNNKGDKTHFI
                     ATGRPIDFLAQLDTAGSKDLHNKQLYTIVDGVRFTGNNGGVTPIWVRPQYGICLNSKC
                     GPK"
     gene            complement(4873..5532)
                     /locus_tag="VIG33_04710"
     CDS             complement(4873..5532)
                     /locus_tag="VIG33_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270166.1"
                     /translation="MKDAQKSPLVILKDLQHNSKGFLSADFLFSLVIASGLCVVFFSL
                     TFTLSMVEVGQYIAYSVSRAHAAAQKTQEDQEKAAKAKFATLQKNKVLRPLFSNGWFE
                     ISNLEIRGAGATGKDFSERYPKEISGVKGVPQVGIRLSFEAKILNLKIPMLGPTDPNG
                     NGFRAFLTGLMIREPSAKECRDQIKNDRYKAILNLDKRFGKVAGANSEQEYVPMEDNG
                     C"
     gene            complement(5541..5807)
                     /locus_tag="VIG33_04715"
     CDS             complement(5541..5807)
                     /locus_tag="VIG33_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270167.1"
                     /translation="MKKNPRTSLLKNEHGQFVVEAVLLMVLSIGLLFTGLRLLKDANV
                     LGNLVAGPWEKVAGMIESGSWEAADKAAKNHPNQIDRSLSVDPK"
     gene            complement(5826..6017)
                     /locus_tag="VIG33_04720"
     CDS             complement(5826..6017)
                     /locus_tag="VIG33_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270168.1"
                     /translation="MTVEYILLLFAVFFIGLKFFTQAPKDAFEKSGPILAMRVEKQLI
                     TGDDFKPNGEKNAWDPEQP"
     gene            complement(6042..6230)
                     /locus_tag="VIG33_04725"
     CDS             complement(6042..6230)
                     /locus_tag="VIG33_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp1 family type IVb pilin"
                     /protein_id="HEY8270169.1"
                     /translation="MKMFKSFSKKLLKNRSGQGATEYILLLVVVVAIVILFKEQIKKS
                     LDGKLSELSGSITNFKGE"
     gene            complement(6558..7076)
                     /gene="hpt"
                     /locus_tag="VIG33_04730"
     CDS             complement(6558..7076)
                     /gene="hpt"
                     /locus_tag="VIG33_04730"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY8270170.1"
                     /translation="MGQIKNDMVPFITRSEIDELVEQLAKQIEYDYEGKEVIFICPLR
                     GSVHFTADLMRKLRLPQQVDFVYVKAVEKGGAIKIVKDISLNIAGKHVLIVEEIIDTG
                     RTLSFLRNRLFASAPASLKIVTLLDKPARRELPIKADYIGKTIDDRYVVGYGMDSEEL
                     GRNYPDIYHLKN"
     gene            complement(7088..7810)
                     /locus_tag="VIG33_04735"
     CDS             complement(7088..7810)
                     /locus_tag="VIG33_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8270171.1"
                     /translation="MKSLNEDMLAEARESFIDGNYKLSEPILQQMLLQNTRNPEVYQM
                     LATIYYDKGQFSKAIKTFKRALEIDPTYTDASVGLSIILNDLGKYDEGKQVFVEAQDQ
                     LEKKNGKNDPFIDEKLSAKHEEIADLYFQYKRYNEALDQLLKAKKLSTRKSEISMRIA
                     EVYVQVGQTDRAIKDLRALIREYPHFIPARLKLGVIYYNSNNIAEATEQWENILMRDP
                     QHPEALRYLKMAQAAGITSIEL"
     gene            complement(7807..8583)
                     /locus_tag="VIG33_04740"
     CDS             complement(7807..8583)
                     /locus_tag="VIG33_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY8270172.1"
                     /translation="MMQTLKSFRILFVNLSAPLLVGQFLLLSLMACSTIDKKSDTPEG
                     AFSIAEEFDKDERYEEAVRRYTEVKNKFPYSNFATKAELAIADVYYKQESYPESQVSY
                     QTFREMHPKHPQIDYVVFRIGMSYYNQLPSSLDRDLSVAQEVVTTFDELMKRFPTSQY
                     INEAAEKKLATLKMLAGKEEYIANFYFKREMFDSALPRYESLIFKYKNLGFDEKAYYG
                     AAVSANKIGDFFKAKKYLAELVTKYPKSSEAEQAQREIKL"
     gene            complement(8618..>9479)
                     /locus_tag="VIG33_04745"
     CDS             complement(8618..>9479)
                     /locus_tag="VIG33_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270173.1"
                     /translation="LPLVQGAESEPEPTSAATTQTPQDKSPVVYQNKTFRSSEDSGLK
                     LSFEEYFSRGTKSYQEKKFEDAVLNFEKALNLHPENVTVLTDLGLSYFQVQKKGLSIA
                     MLRRALFIDPSQTVAEAALKFVFSQLEVKDIPHQIGTYERLRSSILNSVSINALHFLT
                     SLLLFTSCFIWLRYLGRCRKAFELELAPPSTPLIGLMLTLGLTLSGFFTALKIYDLTI
                     PRATVITDKVSVQSAPGEGQNGLFDLYAGFEVIVKNVANDWIQVTYPGGLTGWVKKDS
                     VMSTSSKNAF"
BASE COUNT         2540 a         2047 c         2068 g         2824 t
ORIGIN      
        1 gttctcggat agcgcggata ggaagatcca tccccgacat caagcaaaga gtttcaagac
       61 gcgaaaggca ttcccggggg ctattcgcat gcgtcgtagt cattgatcca tcatgacccg
      121 tgttcatggc ttggagcata tcaagagctg caccatcacg gcactcgccg acaataattc
      181 tgtcagggcg cattctcaga gcgttcttaa taaggtctcg aatagtgacg gcattggatc
      241 cttccatcga agggggacgg gtttctagac gaaccacgtg ttcttgttgc aattgcaact
      301 cggccgcgtc ctcgacagta atgactcgct cattcgaagg aataaatgat gaaagcatgt
      361 tcaacaaaga tgttttacct gaaccggtac cgccactgat caccacattg agtccgtttt
      421 ctacacagat ccttaagaag tcaatcatgg gtttagtgat actgccatat tcagtatatt
      481 tctcgggagt aatcccgcct tttttaaatt tacgaatggt taaggctggg ccatcaatag
      541 caagtggctc gatcaccgca tttacgcgac tgccatcctt gaggcgagca tcgacataag
      601 gtgtggactc attgatctgg cgacctagag ggctcacgat tctttcaatg atacgacgaa
      661 gatgatcatt cgaagtaaag gttaccggac tcatggtgat ctttcctaat ttttcgataa
      721 agattttttt atgtccattc accatgatct cggtcacgtt tggatccgcc aagagatctt
      781 ctaaggggcc taatcccagg gcttcttcta aaacttcttt aataatacga gaccgttcat
      841 ctcgagcttg ctccggagct tcgcgatccg tgatgagggt gatctcacga cgggtttttt
      901 cgcgaacttc tttttcttta ttttcatcgc ccttggtatc cagaagaagt tttttaagat
      961 ccacggtgcg gattaactct gagtgaatac gcaatttcag ttgtgttcgc gaatcatatc
     1021 cactggctgt cccgctcgag ggggccgatg tattgccctc ggctgttggc gcagaggatg
     1081 gttttggccg agagagtgat tttagtttct ggagaactcc tccgctgagt ctacgaaccg
     1141 cttcgtaata agcagaagtg atggctgctt taggttgtcc gatgacaaaa ggttgaaact
     1201 tctgaatcga ggccatggct gttatttcat cctgaggtat gatcgccaag ggcgctagtt
     1261 gcaactgagt ggcgatcgct tgtggcgaaa gtccatttgc tgaagcttta ttgataataa
     1321 gctggaacat gtccttcgga aatgtattgg aaagaagttc attgatcaga cgcaaagtct
     1381 gagtcaccac caaaatttca ggagtggtta caatcataat tgccgtggac tcttgaagta
     1441 tggccctttg caagggagtg aaatcggttc caatatcgac gataataaac ttaaactgac
     1501 gactaaagaa ctccagttgt ttcatggcta agtcgggtcc aatatttaat gtctcctcag
     1561 gaccccgaac agcccccagg taagcaagcc cgctcgcatg catggtcaaa agagaattca
     1621 tattttgcgc atttacagag cttgtcattg aagcgagttc tttaagtgtt ttattgggtt
     1681 tcaaacctag aatgacgttt tgatccccaa cgctcttgct atcgccatca attaaaagta
     1741 cttgagtacg gagttcggcc ataagggcaa aagcaaaatt cgcggcgaaa acactttttc
     1801 ccacgccccc tttaccaccg accactgcta taagattaca acccggattt aatgccaaaa
     1861 gagtcctccg taatacctat cggaggaaag gcggcaaagg gcaagtctag aagtatgggc
     1921 tcaaagtccg cttaatcccg taagcggaat tttttcttga tctgctcagc tccagagctt
     1981 gcggatgttt taatgactgg agtcacaaaa accacgaatt gactctgatt gcgttgaaag
     2041 tttttagaag cataaagact gataattgga tttttcacat cactcggcat gcgattgtat
     2101 cccgtagaag acgcattccg tatcagacca ccgatagcgg cactttgccg atcacgaata
     2161 accacagttg attgaatcga atttgagctg gtaattggag caccgttttg gcctgagccc
     2221 aaaagattgc ttaaagcaaa atccatatcc atatgcacgc tgccggattt ttcgcccaaa
     2281 atgacaggag ttactgcaga tttaataccc acttcagcaa aggcagttcc tggctggcca
     2341 tctttaccga tcacagaata cggcatattt gtaagttgtt taatttcgcc ttttttgcca
     2401 tcttgaatga tcaaactggt gctttctaga atgcgcgcat gtccgtgctc ttttgcccag
     2461 gtaagttttg gcaagagatt agagaccact cccgtgagtg tactcacaac tccaccggcg
     2521 ccactagagc ccgtttgaaa ttgcaattga gagccatcat ctaactgagg agtgaactga
     2581 aatttaaatg ctttagagta atctttattg agttccacat aatgcactac aagttgaatc
     2641 atcttggagg gtggagccgg agcctgttcc ttgatggtaa tcaagttaat gacgccgtca
     2701 tttgcgggtt tgcgtttttt gataacgccc ttttcttccg cagcctcaat gacaatatct
     2761 ggaacataag tctttgcaat aatctctgcg cgcttatatt cctcttcatt attggcgtag
     2821 ccttgcaaaa taaacttgtc attgaccgcg cgaacttcga tttcaggatt attaatatca
     2881 cgtgaaatga attctgcgat tttcttctga gccaatggac ttaaggtgac cagagaagaa
     2941 gcttggtcgc caaattgttg aatgacgtta taaatacggc tgagatcacg tggcaaaaga
     3001 atttgaccat ccacgacgac cttattattc actactttaa ttgtgatgcc ttcaatatca
     3061 cccagaagtg cgcgaatttc ttttacaact ttatcaagct tacttttctt gacatcaatg
     3121 cggaactcag caaccacttt tccggttttt ttatcgtgaa tagtcatggt cgcaaaacct
     3181 tcggctttcg gggtcatgcg cagaacattc aattctttag aatgagccgc ggttacaatg
     3241 cgacggaagt ctcctttgaa ctcaatattt tcaggcaagg gcggaagttt ttcatcttgc
     3301 tcaataccta aagtaagatt gacgaaagtg cgagaacgaa atttagtttc ttccgcttgt
     3361 tcgtcatctt cagcgaaggc atagttacag gatagtacga ggaacagccc caacaagtat
     3421 ttgcacttca tacattcccc ctaagtcagc aaattcaatt cagcgaaatc agttctcaaa
     3481 tttacaaagt cttaaagcca tgcttctttt gcggtaaagt cggtgccgtt ctcggcgccg
     3541 gggccatcat cactggcgga gcaacaggag cggtgggcat tgcatccatg gagatcatcg
     3601 gttttcctaa aacactctcc gcacttgaag taggtagtcg cggtgggatc gtgcgatcat
     3661 ttggatttct caacgttaaa taaatattgc ccggtgccgt tgatagtata taaaccagat
     3721 cctgagcctc tttctgggtc gcctctattg ttattgtact atattttgtg tctcctgtga
     3781 gagcaatttg acttaaagat ttaccactgg agtccaattc aaatacacgt ggaatattgt
     3841 tcataatgtt tacacctgtg gctaaaacta cgacatcctg gaggagtgtt tgtacttctc
     3901 ttttttgatt aatgcctttt ccagtgtcta cagcggctaa aatgtctata cggtcacctg
     3961 ggcgtatcag tttagctacg cctcgcactt catccaccgg aatggtgagg gctcgtttgc
     4021 tgggtgcgat ttgtaatgag attccggtat ctggtcccgg agttaaaaga ctgtttttta
     4081 caatgatctg acctttttta atgggtacag cggcaacgtt acctacaatc tcgtctggat
     4141 ttgaaacagc atcggcatcg acgaattcac tgggacgatc aatagttgtc agcatagtat
     4201 cgtaaactgt gcgcatttcc gcgatgtctt ccttggccac aacgacggtt tttaaggagc
     4261 cgtatttttt attatattca gcgcgctttt cttgagacca gctgtaaagc aagatcgcgg
     4321 caatggctcc ggcacctatt gatacccaaa gatttcttgt ttcatttgat cccatacgct
     4381 tatcccctta ctttggtccg cattttgagt tcaagcagat tccatattgt ggtcgcaccc
     4441 atatcggagt tacgccacca ttgttgccag taaaacgtac accatccacg atagtataaa
     4501 gttgtttatt atgaaggtct tttgaaccag ccgtatctag ctgagctaag aaatcgatag
     4561 gtcgacctgt agcgatgaag tgtgttttgt ctcccttgtt gttactacta acagttgcat
     4621 gaatgcgcat attctgcgat ccaaagctga cgttgtcttc agcagaaaca ccatcacgaa
     4681 gatagaccag attagaacga tgtctgaaag tttcgaaggc gtaatttcgt gcagccatag
     4741 aattcaaaat gccggtatga atcgccccaa agaagcctaa tgaaaaatta aaaaggatga
     4801 cgataataat catgatggga atgatttcca tcacagccat gccttgatta tttcgcagtt
     4861 gtctcgctgg agttaacatc cgttatcctc catcggaaca tattcttgct ctgaattggc
     4921 gccggcaact ttaccaaagc gcttatcgag atttaggatc gctttatatc tatcattctt
     4981 gatctgatcg cggcactctt tagcggaggg ttcacgaatc atcaatcctg ttaaaaaggc
     5041 cctgaagcca tttccgttag gatcggtagg tccaagcatg ggtattttta agttcaagat
     5101 ttttgcctca aagctcaggc ggattccgac ttggggaacg cctttaacac cgctaatttc
     5161 tttaggataa cgctctgaga aatctttgcc tgttgctccg gcaccccgta tttctagatt
     5221 acttatttca aaccagccat tactaaaaag aggtctcaag actttgttct tttgcaaggt
     5281 agcaaactta gctttagccg ctttttcttg atcctcttgg gttttttgtg cggctgcgtg
     5341 agcacggctt actgagtaag caatatattg tccgacttcg accatagaga gtgtaaaggt
     5401 cagggagaaa aaaacaacac agagcccgga ggcaatgacg agggaaaaaa ggaaatccgc
     5461 tgaaagaaag cctttcgaat tatgttgtaa atctttaagt attactaaag gacttttttg
     5521 agcatccttc atagcaccct ctattttgga tccacactta agctgcgatc tatttggtta
     5581 ggatggtttt ttgccgcttt atctgcggct tcccaactgc cactttctat cattccggcg
     5641 actttttccc acggcccagc aacgagatta cctagcacat tggcgtcctt taacagacga
     5701 agcccagtaa ataaaaggcc gatggaaaga accatcagca atacggcttc gaccacaaat
     5761 tgaccgtgtt catttttaag aaggcttgtt cgagggtttt ttttcatact cgggccttcc
     5821 taaggttatg gctgctcagg atcccaagca tttttttcgc cgttgggttt gaaatcatct
     5881 cctgtaataa gctgtttttc gacccgcata gcgaggattg gccctgattt ttcaaaagca
     5941 tctttgggcg cttgtgtgaa aaatttcagt ccaataaaaa atactgcaaa aagcagaaga
     6001 atgtattcca ccgtcataaa gaacctctta gtaaggctaa ctcattctcc cttaaaattt
     6061 gtaatactgc cactcagttc actgagttta ccatctagag attttttaat ttgctcttta
     6121 aacagtataa ctatggccac aacaacgact aagagtagaa tatactccgt tgctccttgg
     6181 ccagatcgat ttttcagtaa ttttttagag aatgatttga acatcttcat agtgttcccc
     6241 cttaggctga ctatcggcta attttgggat ttcctgaaga ggctctggat ttttttaatt
     6301 aaaactgcga gaaactcggg ctgtctcaaa gagagacagc cttcaagatt tctagtaaag
     6361 aactaccgac ttaagtcttt tagctgccag acttattgac gggaaaaagt taccaattta
     6421 gaatcttatt aagatagcta attattaatt ctgcccaggg gaggtaaaag aatgtctggc
     6481 caacttgtaa ttctttacgt tgtttgtgtt gttgctctat ttttcgtaaa cgttgctgtt
     6541 agttaaatca gctcttctta gttcttaaga tggtaaatat ctgggtagtt acgtcccagt
     6601 tcttctgaat ccattccgta tcccaccaca tagcgatcat cgatggtttt gccaatataa
     6661 tcagctttaa taggaagctc gcgacgagcg ggcttatcca aaagagtgac gattttcaaa
     6721 gatgccggcg cagaggcaaa tagacggttt cgtaaaaagc tcaaggtgcg accggtatcg
     6781 atgatttctt caacgataag gacatgcttt ccagcgatat tcagagaaat gtctttcaca
     6841 atcttaatgg ctccgccttt ttcaacggct tttacataga caaagtccac ttgctgcgga
     6901 aggcggagct ttctcattaa atccgccgtg aaatggaccg agccacggag tggacaaata
     6961 aaaatgactt ctttgccctc gtagtcatat tcgatttgtt tggcaagttg ctctacaagc
     7021 tcatcaattt cagagcgggt gatgaaagga accatgtcat ttttaatttg tcccatattc
     7081 tctgccttta tagttcgatg gaggtaatgc ctgcagcttg agccattttt aaatagcgca
     7141 aagcctcagg atgttggggg tctcgcatta ggatattctc ccactgctct gtcgcctcag
     7201 caatattgtt tgaattataa taaataaccc caagctttag gcgagccgga ataaagtggg
     7261 gatattcacg gattaaagca cgtagatctt tgatcgctcg gtcggtttga ccaacttgaa
     7321 cataaacttc cgcaatacgc atggagattt cagacttacg cgtggaaagc tttttagcct
     7381 tcaaaagctg gtccagtgct tcgttgtatc ttttgtattg gaaataaaga tcagcgattt
     7441 cttcatgttt tgcagaaagc ttttcgtcga taaagggatc atttttacca ttcttctttt
     7501 caagctgatc ttgggcttcg acaaagactt gcttgccctc gtcatatttt cctagatcat
     7561 tcagaataat ggaaagaccg acgctggcat ccgtgtaagt agggtcaatt tctagagcgc
     7621 gtttgaaagt cttaatcgct ttactaaact ggcccttgtc ataataaatc gtcgctagca
     7681 tttgatagac ttcaggattg cgcgtatttt gcaaaagcat ttgctgtaaa ataggttctg
     7741 ataatttgta attgccgtcg ataaaggatt cacgcgcttc ggcaagcatg tcctcattta
     7801 atgatttcat agcttgattt ccctttgagc ctgctccgct tcggaagact tggggtattt
     7861 agttacaagt tcagcgagat atttttttgc cttaaagaaa tcaccaattt tattcgcgga
     7921 aaccgcagcc ccatagtacg ctttttcgtc aaaaccaagg tttttatatt tgaaaataag
     7981 ggattcgtaa cgaggcagag cgctatcaaa catttcacgt ttgaaataga aattagctat
     8041 atattcctct ttcccggcca gcatctttaa agtcgcaagc tttttttctg cggcttcgtt
     8101 aatgtattgg gaagtgggaa agcgtttcat caactcatca aaagtggtca cgacttcttg
     8161 agcgacactg agatcacgat ccaagcttga tgggagctgg ttgtaatagc tcatgccgat
     8221 ccgaaaaacg acataatcaa tttgcggatg tttggggtgc atctctcgaa aagtttgata
     8281 acttacttgg gactctgggt aagactcttg tttatagtag acatctgcga tcgcgagttc
     8341 tgcctttgta gcaaagttgc tataggggaa tttgtttttt acctcagtat aacggcgtac
     8401 agcttcttca tagcgttcgt ctttatcaaa ctcctccgcg attgaaaagg ctccttctgg
     8461 cgtatcactt ttcttgtcta ttgttgaaca agccatgagg cttaagagaa ggaattggcc
     8521 cactaataaa ggggctgata aattaacaaa tagaatacga aaagatttga gcgtttgcat
     8581 cataggccct cagtttaact aggggccttt ggtcgagtca aaaggcattt ttgctggaag
     8641 tgctcattac gctatctttt ttgacccacc cagtaaggcc ccccggatag gtgacttgaa
     8701 tccagtcatt tgcaacattt ttaacgatca cctcaaaacc ggcgtagaga tcaaaaagcc
     8761 cattttgtcc ttctcctgga gcggattgta cacttacttt gtcagtaatg acggtcgccc
     8821 tgggaatggt caggtcataa attttcaagg cggtgaagaa acctgagagc gtcaatccaa
     8881 gggttagcat caaaccaata aggggtgtgc ttgggggagc caattctaac tcaaacgctt
     8941 tgcgacagcg ccctaaatag cggagccaaa taaaacagct ggtgaaaagc aaaagggagg
     9001 ttaagaagtg cagggcattt atagaaacag agtttaaaat cgatgaacgt aaacgttcat
     9061 aggtcccaat ttgatgcggg atatctttaa cttcaagctg ggaaaaaaca aatttcaatg
     9121 ctgcttcggc cactgtttgg gagggatcaa taaagagtgc tcgacgaagc atggcaattg
     9181 acaggccttt tttttgaact tgaaaatagc taagtcctaa gtctgtaaga acggtcacgt
     9241 tctccggatg gaggttcagg gccttttcga aattcaagac agcatcttca aacttttttt
     9301 cctgataact ttttgtgccg cggctaaaat actcttcgaa actgagcttt aatccagaat
     9361 cctcagaaga acggaatgtt ttattttgat aaaccactgg gcttttgtct tggggcgttt
     9421 gcgtcgtagc tgcagacgta ggttcaggct cggattcagc gccttgaacg agtggcaaa
//
LOCUS       DATBTE010000126         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1108686, whole genome shotgun sequence.
ACCESSION   DATBTE010000126 DATBTE010000000
VERSION     DATBTE010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108686"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VIG33_04750"
     CDS             complement(<1..215)
                     /locus_tag="VIG33_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270174.1"
                     /translation="MKYKNAEPKVFIIPRSKDLQDPVFHQKAVLHKQLFFILAVAVGF
                     SFSASVAANSMEVEAAIDLLKQSHMEQ"
     gene            complement(831..>1619)
                     /locus_tag="VIG33_04755"
     CDS             complement(831..>1619)
                     /locus_tag="VIG33_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270175.1"
                     /translation="SITFSWEEWNQPFTIQEFLECYKNEMSNIGFKAEINMEEGWASL
                     DIIFFVLDGIVLHRIDEVIHAAKKEYQSLVVKLARAGSENIFVKVFNFPKEYESICTQ
                     YLVWFGEFLLNLGIDADVSAENNRHQTSLIVSPKEAPELLIKIEQLFYQYLSLPYIEY
                     LPAQAVSASVEDKYLLQMLFSQVENFKNQIQMKDAVIEMKELSIASLKENLHEKEKKI
                     LLIQSMKNSKDIEMFGGAISLGEIKLGPLKLDPKKLLDAFKGDV"
BASE COUNT          470 a          361 c          292 g          496 t
ORIGIN      
        1 cattgctcca tgtgcgattg cttcaacaaa tcaatagcgg cttccacctc catgctgttg
       61 gcggcaacag atgcggagaa agaaaatcca acggcgacgg ccaaaataaa aaacaactgt
      121 ttgtgcaaca cagccttctg gtgaaacaca ggatcttgca aatctttact gcgtggaatg
      181 ataaacactt tgggttcggc atttttatat ttcatagtgg tttcccttac ttcaacatta
      241 aagctgagtt gagcagcttg tctgctcaaa cgaacttatg ccctttgggt ataaggattt
      301 tttcatgatc gcctgcccgg attggctagc ggcttacgat gatgacttgc ccgctgggcg
      361 atggcagttc accacggcaa ttggcggcct ttaaaattaa gaaaactgcc gctgttttcc
      421 aaatcaaaac tttcattaag ttccgcatcc ttttcaactg ggtgtggcgc attgtatttg
      481 cccatgtggg ttttaaccca atttggatgc caaatcaaca cagcgacttg ctgtcgatag
      541 agtcaaaagg atgtgctaac gacagatcgt agcttcgaag tcccggattc cgctacgctt
      601 catccaggca cttgctgtat caaaaagtag gctgatttcg cgaagcaaac caccatcaca
      661 cgcacaacac ccaaaacaac aatcgaccat aaaaaatccc cacttggaaa taatcgagcg
      721 atgatcatat ttcgaggctt cgttcgtggt ggattgtctg acagcgaatc caccttacag
      781 cgttagcttg atgcgtatga gttgcaaacc ccgtcccgct cagatgtttt ttatacatca
      841 cctttaaatg catctaacaa ttttttcgga tctaacttta gtggccccag tttaatttca
      901 cctaggctta ttgcgccgcc aaacatttca atgtctttag aattcttcat gctttgaatc
      961 aaaagtatct ttttctcttt ttcatgaagg ttttctttta aagaggcaat agataattcc
     1021 ttcatttcta taacagcgtc tttcatttga atctgattct taaagttttc aacttggcta
     1081 aaaagcattt gaagaagata tttatcttca actgaggctg acaccgcttg tgcaggtaag
     1141 tattcgatat atggcaacga taggtactga tagaataatt gttctatttt aattaaaagt
     1201 tctggcgctt cctttggcga aacgattaac gatgtttgat gtctattatt ttcagccgat
     1261 acatcagcat ctatccccaa gttaagaaga aactctccaa accagaccaa atattgagta
     1321 caaatagact cgtattcttt gggaaaatta aatactttca cgaatatatt ttctgaaccg
     1381 gctcttgcaa gtttcactac caatgattga tattctttct ttgccgcatg aattacctca
     1441 tcaattctat gcaagactat cccatcgagc acaaagaaaa taatatcaag agatgcccag
     1501 ccttcttcca tatttatttc ggctttaaac ccaatattag acatttcatt cttgtagcac
     1561 tccaaaaact cctgaatagt aaatggctga ttccattctt cccaagaaaa cgtaataga
//
LOCUS       DATBTE010000127         2394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1519844, whole genome shotgun sequence.
ACCESSION   DATBTE010000127 DATBTE010000000
VERSION     DATBTE010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519844"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VIG33_04760"
     CDS             <1..180
                     /locus_tag="VIG33_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270176.1"
                     /translation="TNYVDQARRKLISQCPHGEISGISTQYSTSLGFFSWTHKVLMQG
                     LCTKAVADSNRAIHR"
     gene            complement(411..1004)
                     /locus_tag="VIG33_04765"
     CDS             complement(411..1004)
                     /locus_tag="VIG33_04765"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8270177.1"
                     /translation="MVSRILTLEKIISRKSLFLFGPRMTGKTTYLKKNIVIIDEIQKM
                     PELLNEIHKIIEEKKQTRFILTGSSARKLKKVGINLLGGRASEIHMKPLTLFELKEQG
                     FQWTDALSTGLLPSIITSPEPELDLKDYVHLYLQREIKEEALVKNFLNFSRFIDFVNC
                     QPLKAITIKSKLISIFGVLKLNLKLILSWLLIKKSMP"
     gene            complement(1041..1913)
                     /locus_tag="VIG33_04770"
     CDS             complement(1041..1913)
                     /locus_tag="VIG33_04770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEY8270178.1"
                     /translation="MRKSIIFRTFSLMSFMLSGFQTMAVATEKLFIPSHPQIHAPLFV
                     ALHGCMGKAEDSEGRTRMSEFGEKHGFYVFYPEPTSKEDSKGCFDFYSKEAQSPGGGD
                     AIILVNKIQELLKTHDIDENKVFVLGMSGGASLVSVLTSCYPTVFRGAAIHSGIGYGL
                     ASTWQESLLITQTGPLPWHKRNTTCSPSNYKGKMFLIQGSHDQVISPLHFSLLKEDYF
                     SNTKSTSEEISPTFGHYGYIHQTFHQNNQIKGHGILVLGMNHEWSGSKLINPLGPRGP
                     DASSLIVDYFLNSP"
     gene            2159..>2394
                     /locus_tag="VIG33_04775"
     CDS             2159..>2394
                     /locus_tag="VIG33_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270179.1"
                     /translation="MLAGCSGSQGDSLPLIIPTIYYKPIIYKAKENCASSDLKDMIDE
                     NDKVLISLCKNSYDNCLLQGSCFVVEGEKTRAFNV"
BASE COUNT          680 a          472 c          495 g          747 t
ORIGIN      
        1 actaattatg tggatcaagc ccgtcggaaa ttgatttccc aatgcccgca tggagagatt
       61 tcggggatca gcactcagta ttccaccagt ctcggttttt tttcttggac tcataaggtc
      121 ctgatgcaag ggctatgcac caaggcggtc gctgactcca atcgagccat tcatagatag
      181 agccagaaga agattttcct tctgaagacg aatagttgac caatttcaac ctccaagctt
      241 cagccacagc cagtccacaa aatctggtac agaaagaaca tcaacgctat tctgaattga
      301 tgagtgcgaa gagtgggtca ccatcataag atgagcctga ggaaactctt ctttaaaggc
      361 catcagccca gaaaaatctt ttgaacgtgg ctgaagcgat gattttacct ctatggcata
      421 gattttttta ttaacaacca agacaaaatc aacttcaagt tgagtttgag tacgccaaaa
      481 atagagatca atttgctttt gatagttata gcttttaaag gctgacagtt cacaaaatca
      541 ataaaccgtg aaaaatttaa aaagtttttt accagagctt cttcttttat ttccctctgt
      601 aaatacaaat gaacataatc ttttaaatcg agctctggtt cagggcttgt aatgatactt
      661 ggaagtaaac ctgttgaaag ggcgtcggtc cattgaaatc cctgttcttt aagttcgaac
      721 aaggtgagag gtttcatgtg aatttccgaa gctctgcccc ctaataagtt aatgcctact
      781 ttttttaact ttcgagcact gcttcccgtg aggataaatc ttgtctgctt cttttcttca
      841 attattttgt gaatttcatt gagaagttcc ggcatttttt gaatttcatc aataatcaca
      901 atattttttt ttagataagt ggttttcccg gtcatcctgg gaccaaataa aaaaagactc
      961 ttcctgctaa tgattttttc taaggttaaa atgcggctta ccatggagtc tatgaccagg
     1021 ttataggcta aaacactgtt ttacggagaa tttaaaaagt aatcgacgat caggctggaa
     1081 gcatcgggac cccgtggtcc gagaggattg ataagcttgg aaccgctcca ttcatgattc
     1141 attcctaaga ccagaatgcc atgacccttt atttgattgt tttgatgaaa ggtttgatga
     1201 atgtagccat agtgaccgaa tgtgggggag atttcttcgg acgtcgattt cgtatttgaa
     1261 aaataatcct cttttaggag ggaaaaatga aggggactta tcacttggtc gtggcttcct
     1321 tggatcaaaa acatttttcc tttgtagtta gagggagagc aagtggtatt tcttttgtgc
     1381 caaggcaagg gaccggtttg agtgattaaa agtgattctt gccaggtgga agcaagtcca
     1441 taacctattc ctgaatggat ggcggctcca cgaaagacag tgggataaca actggttaat
     1501 acactcacga gagaagctcc ccctgacata ccgagtacaa agactttatt ctcatcgata
     1561 tcgtgggttt ttaatagctc ttgaatctta ttcacaagaa tgatggcatc gccacctcct
     1621 ggactttgag cctctttgga ataaaaatca aaacatcctt ttgaatcttc tttggatgtg
     1681 ggctccggat aaaaaacata aaatccatgt ttttcaccga attctgacat tcgtgttctg
     1741 ccttcgctat cttcagcttt acccatgcat ccgtgtaaag ctacaaaaag gggagcatga
     1801 atctgaggat gggaggggat aaatagtttt tccgtggcga cggccattgt ttggaaacca
     1861 ctcaacatga aactcataag actgaaagtt ctaaaaataa tactttttct caaagtcttc
     1921 ccccctggta ttcattttca ttacctaggt ttcggtcgag ccgaccttaa tctggagggg
     1981 agttatggaa attatttacg tcttcgacag ttccattatt gttttggaaa aactgcggaa
     2041 gttttagacg aaattttcat tgcaccacga taatatccgc attggacttc atgtcccggc
     2101 atgttagccc tgtcgtttca tggttcacaa tttttcattg ctttttataa gcttattttt
     2161 gttagctggc tgttcgggaa gtcagggtga ttctttacct ctgattattc cgactatcta
     2221 ttacaaacca attatttata aagccaaaga gaactgcgct agctccgatc ttaaagatat
     2281 gatcgatgaa aatgataagg ttctgatttc cttatgcaag aattcttacg acaactgcct
     2341 cttgcagggg tcctgttttg tcgtggaggg cgagaaaacg cgagccttca atgt
//
LOCUS       DATBTE010000128         1660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1997242, whole genome shotgun sequence.
ACCESSION   DATBTE010000128 DATBTE010000000
VERSION     DATBTE010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997242"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VIG33_04780"
     CDS             complement(<1..434)
                     /locus_tag="VIG33_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270180.1"
                     /translation="MKLALIPLEEWERLNDQLKIDFSLPAGNSARIYKGMAHAVFETA
                     QGTAQFFSHKRSLGVVKGQTSYFDSLLPYFYKEVFSVQAMSHLAVVDPEVWVNSLKKD
                     TSFVLFSEDHPVTGELYDYDHLDELLNEKKIFAFRISHFRHF"
     gene            659..1273
                     /locus_tag="VIG33_04785"
     CDS             659..1273
                     /locus_tag="VIG33_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270181.1"
                     /translation="MNRLLICMFTIIMGSIISHPAHALFDARLTYGLLASKPDMTALF
                     PGQATVPAIAPTYGLGADVIVSPPLFPLGFGLRYENMGVSASSGSLEFKANYTRTAVL
                     INYRIIDTLLFLGPIFSYGLSHSGSVKAINGGTEIANFSSSKISSYSVGLEAGAKLIG
                     FNIGAEAGIQDFRWKDAQDSTNTLATQDLNLSGSYMKVLVGFGI"
     gene            complement(1360..>1660)
                     /locus_tag="VIG33_04790"
     CDS             complement(1360..>1660)
                     /locus_tag="VIG33_04790"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY8270182.1"
                     /translation="KKKHKLKLQGTEFQMKVWLELINIPKGVTVTYSQVAQKIKKPKA
                     VRAVGTAVGQNPICVWIPCHRVVGKNGNIFKFRGGAKTKVGLLKAEGSISNEILP"
BASE COUNT          469 a          371 c          336 g          484 t
ORIGIN      
        1 taaaaatgcc gaaagtgtga gattcggaaa gcgaaaatct ttttctcatt caataattca
       61 tcaagatggt cataatcata aagctcccca gtgacgggat gatcttctga aaatagaaca
      121 aagctggtat cttttttaag actattcacc caaacctcgg gatcgaccac agctaaatgg
      181 ctcatagctt gaacactgaa aacttctttg taaaaataag gaagcaacga atcgaaatag
      241 gacgtctgcc ctttaacaac accgagactg cgtttgtgag aaaaaaactg agcggttccc
      301 tgagctgttt caaaaactgc atgagccatt cccttataaa tcctcgcgga attaccggcg
      361 ggcaaagaaa aatcaatctt caattgatca ttcaatcttt cccattcttc caatgggatg
      421 agagctaatt tcataaacaa agggtaaatc agctgtgacc gcttgtctag agaacaagga
      481 tatgtagcgt aagtaaaggg gcaggtatca tgaaatatcg ttttacatta taatttgaaa
      541 ttctatggag cactttagac caaggtctta attcgatttt gcaatggacc ctcaagagaa
      601 ggccgagata gaataaaaac ctgagttata actttgaact accaggagga ccatccgtat
      661 gaaccgactc ttaatttgca tgtttacaat tattatggga tccatcattt cacatccagc
      721 ccacgctctt ttcgatgcgc gactcacata tgggcttctt gcttctaaac ctgatatgac
      781 tgccctgttt ccgggccaag ccactgtgcc cgcgatcgct ccaacttatg gtctgggtgc
      841 ggatgtgatc gtttctccac ccctttttcc ccttggtttc ggtttacgct atgaaaatat
      901 gggtgtcagc gccagcagtg ggagcctgga atttaaagcg aattacactc gcacggcagt
      961 gctcataaat tatcgcatta ttgatacttt actgtttttg ggacctatct tcagctatgg
     1021 actttctcac tctggaagcg ttaaagcgat caatggcggc accgagattg caaacttcag
     1081 ctctagcaaa atctcttcct atagtgtcgg tctagaagcc ggcgccaaac tcattggctt
     1141 caatattggt gccgaagcgg gtattcagga tttccgctgg aaggacgctc aagacagcac
     1201 caacactctg gctactcaag atcttaactt gtctggttcc tacatgaaag tactggtagg
     1261 tttcggtatt taatttattt cgatccaaat aaatataaaa caggattaaa ctaaaaaaag
     1321 attggaagcc caatcttttt ttagtttatc acctcatggt caaggcagta tttcgttaga
     1381 gatcgaccct tcggctttta agagacccac ttttgtcttt gctccaccac ggaatttaaa
     1441 gatatttcca tttttgccca ccactcgatg gcaaggaatc catacacaga tcggattttg
     1501 gccgactgcc gtgcccaccg cccgtactgc tttgggtttt ttaatttttt gagccacttg
     1561 agaataggtt acggtgacgc ccttggggat attaataagt tcaagccaca ctttcatctg
     1621 aaattcagtg ccctgaagct tgagcttgtg ctttttcttc
//
LOCUS       DATBTE010000129         6518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_631658, whole genome shotgun sequence.
ACCESSION   DATBTE010000129 DATBTE010000000
VERSION     DATBTE010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631658"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..789)
                     /locus_tag="VIG33_04795"
     CDS             complement(61..789)
                     /locus_tag="VIG33_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8270183.1"
                     /translation="MIQFKNLVKRFGDRTVLKGLNLDIREGEILFILGTSGTGKSVLL
                     KNLVGLLRPDEGEIWIDGKEVSQLSEEEYLPIRKKCGMVFQHPALFDSLTIYENVAFG
                     LRRHYQLDEEVIIHKVKKALSLVHLSGIEYKKPVQISYGMQKRVSLARTVALEPRILL
                     FDEPTTGLDPITTNAVNQLIFDLSRDLRTTSLVVSHDMQCALSIADRIVVLDKGEIVA
                     LGTPDELKKSQVSLVKDFLAEVSL"
     gene            complement(844..1599)
                     /locus_tag="VIG33_04800"
     CDS             complement(844..1599)
                     /locus_tag="VIG33_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8270184.1"
                     /translation="MSLKLQGIEVSFDSQTILKNVSVEIPAGEVFVLMGPSGHGKTTL
                     LKTMAGLITPRNGKVFINQQDWSTLGDNERFETLKKLGMLFQKNALFDSLTCGENIAF
                     PLRETTDLSPEQIQTKVDDFLEAVGIAHAKFLYPDEISGGMQKRLGIARALALNPEII
                     FYDDPTAGLDPITSKKIIELILDLQRKNKSTVVAITNDVNRAYQLANRMALVIEQELI
                     VTGTPEETKKHPDPRVQQFIRGSLKGPLTEVHS"
     gene            1716..2660
                     /gene="mdh"
                     /locus_tag="VIG33_04805"
     CDS             1716..2660
                     /gene="mdh"
                     /locus_tag="VIG33_04805"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY8270185.1"
                     /translation="MAHKRNKIAVIGAGFVGSTTAHWAAQKELGDVIVLDINEGTAIG
                     KSLDLAQAAPIELFDSRIKGTSKYEDIADSDVVIVTAGMPRKPGMSRDELVGINAKIV
                     KDVCDGIKKYAPNSFVIVVCNPMDVMAVYAKQMLGFPRERIVGMGGCLDSARFRAFIA
                     EELNVSVKDVTGIVLGNHGDAMVPMVRMASVSGVPLMDLMPAEKIAAIVARTKTAGAE
                     IGGHLKTGSAFYAPSRGAVEMAEAVLKDQKRILPVAVELNGEFGVTDKLMVGVLARLG
                     GKGMEGIIDIKMNADEKAEFDKSVEAVRTLVSALKNVQ"
     gene            2682..3845
                     /gene="sucC"
                     /locus_tag="VIG33_04810"
     CDS             2682..3845
                     /gene="sucC"
                     /locus_tag="VIG33_04810"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEY8270186.1"
                     /translation="MNIHEYQAKEVLRKFGVATLKGKVAHSPEEAIAAAKEMGGTLWV
                     VKAQIHAGGRGKGGGVKIAKNLDDVGELTKKMIGMTLVTHQTGPEGKVVQKVFIEQGW
                     NIAKEYYVACLIDRATGRAAMMASSEGGMDIEEVAEHNPNAIKKVAIDPTIGLAPFQA
                     RELAFQIGMAPEHVNKAVKFFTGLYNAFVTTDCSIAEINPLVVTKEGEVLCLDAKMNF
                     DSNALYRHQDIVEMRDLNEEEPSEIEASKYDLAFIKLDGNIGCLVNGAGLAMATLDII
                     KLHGAEPANFLDVGGGANKEKVTAAFKIILKDPNVKGILVNIFGGIMKCDIIAEGVIS
                     ASKELGLKVPLVVRLEGTNVELGKKMLRESGLNITPADNLTDAAKKIVAAVKG"
     gene            3851..4720
                     /gene="sucD"
                     /locus_tag="VIG33_04815"
     CDS             3851..4720
                     /gene="sucD"
                     /locus_tag="VIG33_04815"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEY8270187.1"
                     /translation="MAILIDKNTKVICQGFTGAQGTFHSEQALAYGTKMVGGVTPGKG
                     GTTHIGLPVFNTVKEAKAATGCNASVIFVPPPFAADSIMEAVDADLDLVICITEGIPV
                     LDMVKVKKFMQGKRTRLIGPNCPGVITPGACKIGIMPGSIHKPGRIGVLSRSGTLTYE
                     AVGQLTALGLGQSTAVGIGGDPVNGTNFIDMLKLFNEDPDTDAVIMIGEIGGTAEEEA
                     AEYIKKYFKKPVAAFIAGAAAPAGKRMGHAGAIISGGKGTAEAKFAALEAAGCKISRS
                     PADMGTTLKSMLK"
     gene            complement(4793..5158)
                     /locus_tag="VIG33_04820"
     CDS             complement(4793..5158)
                     /locus_tag="VIG33_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270188.1"
                     /translation="MTKNMLLIFATMTFSSFALADVIKCSFTEPFITTVYSMAQQTLA
                     TSNMGEKPEVIKNVSFQIKGPGKFELVGKDGKVLQELTLNQNGSDGMSEIVYPYEAKA
                     GPLFGGCSSNYLKAKNTEH"
     gene            5439..5891
                     /locus_tag="VIG33_04825"
     CDS             5439..5891
                     /locus_tag="VIG33_04825"
                     /inference="COORDINATES: protein motif:HMM:NF022458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HEY8270189.1"
                     /translation="MKKISTLFAGLILSLGSSLCRATLGEVINSPSTTLSTNNSSQKI
                     STSGKFITHEYEKDGNTIREYASESGVVFAVSWRGISKPDLNTLFGSYFNEYKQALDE
                     VPKQYGVKTISMKTAKMMVRRGGRMRDPRGFAYIPSMVPEGVNIEGLP"
     gene            5888..>6518
                     /locus_tag="VIG33_04830"
     CDS             5888..>6518
                     /locus_tag="VIG33_04830"
                     /inference="COORDINATES: protein motif:HMM:NF023352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 family protein"
                     /protein_id="HEY8270190.1"
                     /translation="MNTRVLILSLFSIISLLAYNNCAKKGFEAVDSAVTTTTGGSDSE
                     PLPIIGNEANVMKLTVGCSYINQPCVSVTICEPGTSNCQTIDKILLDTGSYGLRVFSS
                     LVNINLPQKTISGGDLAECISYADGSSNWGPIKTADVVLGSLKAPGIPIQTIDSTYAT
                     LPSDCTKPDTNPSTAGYNGILGVGIFTEDCGAGCATVQNNRIYFACNIST"
BASE COUNT         1853 a         1358 c         1464 g         1843 t
ORIGIN      
        1 aaatcaaact ttttccgatc tcgtcagcga tttggatcaa acttcgaatg ggcatcatca
       61 tcatagtgac acctccgcca aaaagtcctt cactaatgag acttgggatt tcttcaattc
      121 atcaggagtt ccgagggcaa caatttcacc tttatctaaa acaacaatgc gatcggcaat
      181 actgagagcg cattgcatat cgtgacttac cactagagaa gtggtccgta ggtcacgcga
      241 aagatcaaaa ataagctgat tgacggcatt tgtggtaatt ggatcgagac ctgtcgtggg
      301 ctcatcaaaa agtaagattc taggttctaa ggccacggtt ctggcaagac tgacccgctt
      361 ttgcatccca taagaaatct ggacaggttt tttgtattca ataccactca gatgaaccag
      421 actgagggcc tttttaactt tatgaatgat cacttcttcg tccaactgat agtgacgacg
      481 caaaccaaat gccacgttct cataaattgt taaggaatca aaaagcgctg gatgctgaaa
      541 aaccattccg cattttttac gaatgggtaa atattcttct tcgctgagtt gactcacttc
      601 ttttccatca atccagattt caccctcatc cggacgtaaa aggcccacaa gattttttaa
      661 gaggactgac ttgccggtgc ctgatgtgcc caaaataaat aggatttccc cttcacggat
      721 atcaaggttc aggcctttta gaacggttcg gtctccgaac cgtttaacga gattcttaaa
      781 ttggatcatt ccaacatgat atagaggccg ttgcctaaaa tctattttta gtaattaagc
      841 cccttagcta tggacttcag tcaagggccc cttgagagag cctcgtataa attgctgaac
      901 ccgaggatca ggatgtttct ttgtctcttc gggagtaccg gtgacaatta actcttgttc
      961 tatgaccaag gccatacgat ttgcaagttg ataagcacga ttcacgtcgt tagtaattgc
     1021 aaccacagtt gatttgtttt ttctctgtaa gtctaaaatc agttcaataa ttttttttga
     1081 agtgatcgga tccaagcctg ctgtggggtc atcataaaaa ataatctcgg gattcagagc
     1141 taatgctcta gcaataccca agcgcttttg catgcctccg ctgatttcat ccggataaag
     1201 aaactttgca tgagcgattc ccacagcctc aaggaaatca tccacttttg tttggatctg
     1261 ttccgggcta aggtcggtag tttccctcag aggaaacgcg atgttttcac cacaagtgag
     1321 agaatcaaaa agcgcattct tttggaaaag catacctaat tttttaagcg tttcaaagcg
     1381 ctcattgtcg cccagcgttg accagtcttg ttgattaatg aaaactttgc cattccgagg
     1441 agttatcaaa cctgccatgg ttttcaagag agtggttttg ccgtgaccac tagggcccat
     1501 taatacaaaa acttctcccg caggaatttc gacagagaca ttttttaaaa tagtttgcga
     1561 gtcgaagctc acttcaatcc cttgtagttt caaagacata aatgcgatga ctcacctcac
     1621 tcggacttgt ctgaatactc gatttaagat tgaataagcc cctgaaagca atctaatttt
     1681 taattactta ttcattctta atctaaggag acatcatggc tcataaaaga aataaaatcg
     1741 ccgttattgg tgctggtttc gtaggttcaa caactgctca ttgggcggct caaaaagagt
     1801 tgggcgatgt tattgttctg gatattaatg agggcaccgc tattggtaaa tccctggatt
     1861 tagctcaagc tgctccaatc gaattatttg attcccgtat caagggaact agcaaatacg
     1921 aagatatcgc agactctgat gtggtgatcg tgaccgcagg gatgccccgt aaaccaggta
     1981 tgagccgcga tgaactcgtg ggaatcaatg caaagatcgt taaagatgtt tgtgatggca
     2041 ttaaaaaata tgctcctaat tcctttgtca tcgtggtgtg caatccaatg gacgttatgg
     2101 cggtttacgc aaaacaaatg ttaggtttcc ctcgtgagcg cattgtgggt atgggcggtt
     2161 gtttggattc agctcgattc cgcgccttca ttgctgaaga attaaatgtt tccgtaaaag
     2221 atgtaactgg tatcgtgcta ggtaatcatg gagacgcgat ggtccccatg gttcgcatgg
     2281 cttccgtcag tggtgttccg ttaatggatt taatgcctgc tgaaaaaatt gccgctatcg
     2341 tggctcgcac caaaacagcc ggtgccgaaa tcggtggcca cttgaaaact ggctcggcat
     2401 tctatgctcc ttctcgtgga gcggttgaga tggcggaagc ggttttgaaa gatcaaaaac
     2461 gtattcttcc agtcgctgtt gagctcaatg gtgagtttgg tgtcactgat aagttgatgg
     2521 tcggtgttct tgctcggctt ggcggcaaag gcatggaagg tattattgat attaaaatga
     2581 atgcggatga aaaagcagaa ttcgataaat ctgtagaagc cgttcgcact cttgtttccg
     2641 ctcttaaaaa cgtacagtaa ttaattgaaa ggctcctata aatgaatatt catgagtatc
     2701 aggcgaaaga agtcttgaga aaattcggtg tggcgacttt aaaaggtaaa gttgctcatt
     2761 cccctgaaga agcaatcgca gcagctaaag aaatgggtgg aactctttgg gtcgtaaaag
     2821 ctcaaatcca tgctggcggc cgcggtaagg gcggcggtgt taaaattgca aaaaacttgg
     2881 acgatgtcgg cgaactgaca aagaaaatga tcggtatgac tttggtaact caccaaaccg
     2941 gccctgaagg caaggtggtc caaaaggttt tcattgaaca aggctggaat attgcaaaag
     3001 aatactatgt ggcttgctta attgaccgcg cgacaggaag ggctgcaatg atggcttctt
     3061 ctgaaggcgg aatggatatc gaagaagttg cggagcacaa tccaaatgca attaaaaaag
     3121 tagctatcga tccaacaatt ggcttggctc ctttccaagc tcgtgaattg gccttccaaa
     3181 ttggcatggc accagagcat gtgaataaag ccgtgaaatt ctttacaggt ctttacaatg
     3241 ccttcgtcac tacggattgc tctattgctg agatcaaccc gctggtagtt accaaagaag
     3301 gtgaagttct ttgcttggat gcaaaaatga atttcgattc gaatgctctg tacagacatc
     3361 aagatattgt cgagatgcgc gatttaaatg aagaagaacc ttcggaaatt gaggcttcta
     3421 aatacgatct agcgttcatt aaactcgatg gaaacatcgg ttgtttggtc aatggcgctg
     3481 gtttagcgat ggcgactttg gatattatca aacttcatgg agcagaacct gcaaacttct
     3541 tagacgttgg cggcggcgct aataaagaga aagtaacagc ggctttcaaa attattctta
     3601 aggatccaaa tgtaaaaggg atcttagtga atattttcgg tggtatcatg aaatgtgaca
     3661 ttatcgctga gggtgtcatc tctgcttcca aggaattggg actcaaagtt cctctcgttg
     3721 tccgcttaga aggtaccaat gtggagcttg gtaagaaaat gctgagagaa agcggattaa
     3781 acattactcc tgctgacaac ctcacagatg cggctaaaaa gatcgttgct gcggtaaaag
     3841 gataattaag atggcaattc taatcgataa aaatacaaaa gttatttgcc aaggcttcac
     3901 aggagctcaa ggaaccttcc attctgaaca agctctggct tatggcacca aaatggttgg
     3961 tggagtaact cccggtaaag gtgggaccac ccatattggt cttcctgtat tcaatactgt
     4021 gaaagaagcc aaggcggcca ctggttgcaa tgcttcggtg atctttgtcc ctcctccctt
     4081 tgccgcagat tccatcatgg aagctgtgga cgcggacttg gatttagtaa tctgtattac
     4141 agagggcatt ccagttctcg atatggtaaa agtaaaaaaa ttcatgcagg gcaaacgcac
     4201 tcgcctgatc ggtcctaact gcccaggtgt tatcacccct ggcgcatgta agatcgggat
     4261 tatgcctgga tctattcaca agccaggtcg catcggcgtt ctttctcgct caggcacgtt
     4321 gacctatgag gctgtgggac aattgacagc tttgggtctt ggtcaatcca ccgcagtcgg
     4381 tatcggtggc gatcctgtca acggaacaaa ctttatcgat atgctgaaac tcttcaatga
     4441 agacccagat acagatgcgg tgatcatgat tggtgaaatt ggcggaacgg cagaagaaga
     4501 agctgctgaa tacattaaaa agtatttcaa aaaacctgta gcggctttca ttgcaggcgc
     4561 ggctgctcca gcaggaaaac gcatgggcca tgcgggcgcg atcatcagtg gcggcaaagg
     4621 aactgcagag gccaaattcg cagcccttga ggccgcaggt tgcaaaattt caagaagtcc
     4681 agcagatatg ggcactactt tgaaatctat gcttaaataa tttggtattc tttagaaaga
     4741 aaaaacccac gctatcgaag cgtgggtttt tttattgaat aaatctataa aattaatgtt
     4801 ctgtattttt tgccttcaaa taatttgatg agcatccccc aaatagaggg ccggctttag
     4861 cttcataagg ataaactatc tcactcattc catcagagcc attttgattt aaggttagct
     4921 cctgaagaac tttaccgtct ttacctacaa gttcaaactt tccagggcct tttatttgaa
     4981 aggaaacgtt tttaatcact tctggttttt cgcccatatt acttgtagca agagtttgct
     5041 gggccatgct gtagactgta gtaataaacg gctcagtgaa cgaacatttt atgacgtcag
     5101 ccaaagcgaa gctagaaaac gtcatggtcg caaagataag gagcatgttt tttgtcattt
     5161 tgtttctcca ggtttatggt attgtgttta gaggggcata acatacagtt aaatgttctt
     5221 tcatgcttta tgaaaaatgt taccttggaa tatttcccag aagcgtctag aagtttaaca
     5281 gttaattata gatgaagccc gattaggtca aatcaaggtt tcctttaatt gtctcaaaac
     5341 aacacgcccc tggaagaaaa tccctgcctc tgtatattgc ggcactcctt cccccttgct
     5401 aaacttaaag aatgaccgat aaattctaaa aggggcctat gaaaaaaatc tctactctat
     5461 ttgctggtct tattctatct ctgggttcct ctttgtgccg ggcaaccctg ggtgaggtta
     5521 ttaattcccc ctccacgact ctttctacta ataattcttc tcaaaaaatt tcgacctctg
     5581 gaaaattcat aactcatgag tatgaaaaag atggcaatac aattcgtgaa tacgcctctg
     5641 aatcaggggt cgtctttgct gtaagttggc gaggcatttc aaagccagac ttaaacacgc
     5701 tttttggctc ttatttcaat gagtataagc aagcattgga tgaagtacct aagcaatatg
     5761 gggtaaaaac tatctccatg aaaactgcaa aaatgatggt tcgacgggga ggccgcatgc
     5821 gtgacccaag gggctttgcc tatattcctt cgatggtgcc ggagggcgta aacatagaag
     5881 gcttaccatg aacacgcgcg ttctaatact gagccttttt tccataatat ccttattggc
     5941 ttataacaac tgcgccaaga aaggttttga agccgtagat tctgcagtaa ccacgacaac
     6001 cggcggctct gacagcgaac ctcttcccat tatcggaaat gaagccaacg taatgaaact
     6061 cactgtgggt tgcagctata ttaatcaacc ttgtgtgagc gtgactatct gcgaacctgg
     6121 aacttctaac tgccaaacga ttgataaaat tctccttgat acaggaagct atgggcttcg
     6181 cgtattttca agcctagtga atataaatct ccctcaaaaa actattagtg gaggggatct
     6241 cgcagagtgc atctcttatg ctgatggtag ttcaaactgg gggccgatta aaactgcgga
     6301 tgttgtttta ggctcgttaa aggctccagg catccctatt cagacaattg actctactta
     6361 tgccactctt cccagtgatt gcacaaaacc agacacaaac ccctcaacgg ccggttataa
     6421 tgggattctc ggtgttggga tttttacaga agattgcggt gctggttgtg caactgttca
     6481 aaacaatcgc atttatttcg catgcaatat aagtacct
//
LOCUS       DATBTE010000130         8342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1109750, whole genome shotgun sequence.
ACCESSION   DATBTE010000130 DATBTE010000000
VERSION     DATBTE010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1109750"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VIG33_04835"
     CDS             complement(<1..256)
                     /locus_tag="VIG33_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270191.1"
                     /translation="MNLLSKIFFICFSFCTFISCSYLQIIREKPKAKIVSILPDGVTP
                     FAIKFRSEIEVENPYTFSIEINKINFKFTSENKKLVEGNSN"
     gene            471..893
                     /locus_tag="VIG33_04840"
     CDS             471..893
                     /locus_tag="VIG33_04840"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY8270192.1"
                     /translation="MEPEKSKKRRAKKLRQNETQEILTPEFDELTDQELVLRDQEIAL
                     HSPQESDYTTLEPPSGSEPEKSFYTLTDKDLLEEVVELLDRHPEIDATEIKVQVEKGE
                     VILTGGVPEEKMKLLSAEVIKLLPGVKVVSNQLRLLNP"
     gene            1074..1808
                     /locus_tag="VIG33_04845"
     CDS             1074..1808
                     /locus_tag="VIG33_04845"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY8270193.1"
                     /translation="MKKSLYSALLLIGISLVPLCGFAGFDMNAVKDLYQPTQRSRLYS
                     KDPAYTPNYRFGIKYTFMPYQDPDMHEAIDTNNPLLNPAYDSYNLIVIVNKFDRRDQW
                     GRAQTMRVYKRNVKENDGLIYYWYVSTGMDGFGTNAGYFVPQSFSSRHWSSQYDAPMR
                     SSVFFLGGKALHASIDSDSLKALGTPYSHGCVHVEDNRAEELFHLIGHSGYGSVDLID
                     QKTGKLKLENGRAIKVQATKTLIIVH"
     gene            2075..2347
                     /locus_tag="VIG33_04850"
     CDS             2075..2347
                     /locus_tag="VIG33_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270194.1"
                     /translation="MPFGSGTNVNSVSKLWTCEAHPKKLKGVLYKFRERWQPYTYTYA
                     YAYAYAYTYAYAYTYAYAYAYAYAYAYAYAYAYAGTDTDSCPRDFD"
     gene            2364..2789
                     /locus_tag="VIG33_04855"
     CDS             2364..2789
                     /locus_tag="VIG33_04855"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HEY8270195.1"
                     /translation="MGNFVVDQYFSGGMTYANVSGAINVDTSGVLYQAPQAVYHCERY
                     GNFSYTIPGLTPGVAYEVRLHFVEGYWQAVGQRIFNVSINGTKVLSNFDIFATAGSMN
                     KAVLQTFLMAPSSDGTMLINFTSIIDNAKVDGIEVLLKH"
     gene            2868..3401
                     /locus_tag="VIG33_04860"
     CDS             2868..3401
                     /locus_tag="VIG33_04860"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEY8270196.1"
                     /translation="MMSHGSTKEKDKHRQYRQSLGDQVSTIEQEARMLLPGIQTLFGF
                     QLISVFNPGFKASLSEEEQIIHLIALLLVAISAVLVVAPAAYHRQAKHQISEHFIELS
                     SNFLAWSMAPVALGTCLDIYLVTRVILVSHLYSFIITLTIFTFYAWTWFVLPKRRAKK
                     IRFLPTHELNEEKKSHI"
     gene            3479..4804
                     /locus_tag="VIG33_04865"
     CDS             3479..4804
                     /locus_tag="VIG33_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270197.1"
                     /translation="MRKLTSRMGSILKLSLCILMVINSQVHAYSPKAPVLEDDLDARS
                     TLVRERLLIEYAEIKSASQFIEKFAPQRPAAEKVELLKRLSKLKRLPKLEAIANGLRL
                     TQDSMSFDMDLQSISKRRFLINGQVFTFSPNHSWVIQSEILWDKLQHPRKSSYSLFSL
                     LIPEAHGVVMQTIMAFGYTVILMGLVNTAIGHYGNDGFNVLDWEVCERVRKSNNGEFP
                     RNPRFCKDYRREVEEQAKKMPGANAVQVALTDAGSKSEKYSTSNETCPDQNKDQHYNG
                     TVTIVSKEEPTQRNKDVKNEVVKSNEGLKLVRVYAEVDGTRLKSASVYHDDLVKGESK
                     SKLLATYVVDGNSVLEEIVIPASSKINSKLSKEKLTTRISASADLKDKPDLQAQQAFF
                     QGLFSNLGDRLKFCKMQKENKEAKEAVKKVEKGPRESAGTPTGGNTGHW"
     gene            4958..5815
                     /locus_tag="VIG33_04870"
     CDS             4958..5815
                     /locus_tag="VIG33_04870"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY8270198.1"
                     /translation="MKLCAYVRSLLMFCVIFLLGAMSYAHEMKVMTFNIRCGSCEDSS
                     SINHWSKRKSLVRELIKSQAPDIIGLQEAEQFQIKDLANLLGDYDWIGVGRDDGKEAG
                     EATVIFFRKSRFSLLTQKTLWLSDTPEVVSRGWDGKLNRTVTILKLKDNNSNGREFYF
                     YNTHLDHVGQQARAEGLQLIVRLMNSGSENLPLILTGDFNFTRDFWAYNIIASRLLDA
                     QFISQSPPEGGNQTFNGFGSSTDPNNKIDYIFVSDSWNVISHRISTQLYEGRYPSDHF
                     PVEARLTLK"
     gene            6015..6091
                     /locus_tag="VIG33_04875"
     tRNA            6015..6091
                     /locus_tag="VIG33_04875"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6049..6051,aa:Met,seq:cat)
     gene            6327..6797
                     /locus_tag="VIG33_04880"
     CDS             6327..6797
                     /locus_tag="VIG33_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270199.1"
                     /translation="MKEILETLKKACGVYLGEGINHEGQPFTGRLSLEPLLAGRGFQI
                     QFSATGKDGKLYHQEASTIAPAMTEKLTLWNFNTNIPGLVPHELRSAIAKPSAISSLV
                     FGFNSPQDDKAFREEVALDIWDSNSISYTYSWGMPGGEFKERSGVRMVKQTEKP"
     gene            6794..7633
                     /locus_tag="VIG33_04885"
     CDS             6794..7633
                     /locus_tag="VIG33_04885"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8270200.1"
                     /translation="MNTSIETFLQDFHKKHPGCTPAAFSNGFTKDHKTSYDMVASVLA
                     PSSENQAIVLDLACGDGVLLQRISTAGYKNLNLIGVDMSVGELEAARTRLSSAKVKLL
                     EAKAQSLPLPDNSVDFVLCHMAFMLMDGIENVVSEIHRVLKPGGTFSAVVGGKYEKAP
                     AMDAFLQLLDEALKEESQSWLSNLGDKRTRSEDGLRSLFTKDRFVQSGTVQDFSIRFH
                     DRPENLMDFFMLMYDVAALSPARQVKLGGDLLLKLESLSDEMGKMHHSMGLRQIIFCK
                     GDS"
     gene            7637..>8342
                     /locus_tag="VIG33_04890"
     CDS             7637..>8342
                     /locus_tag="VIG33_04890"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEY8270201.1"
                     /translation="MRTPDHLKIKETCRLAILPLMTKHGLGSPINILVDETGWVNPCF
                     FVNDKFVFRFNARDPELPKYQREKFAFDLLKTSTVPVPQKVILDETKTSAPFDVLITE
                     MIPGENLETAWPKLNTEQRENLAENAGKLLKQMTTMQLPLFGELASTGPFPKTETWVS
                     YLQAKLSFHLGEALSLEIFDRKVIEKFWNTFNKHVSALSEVSIPRLVHVDFHFGNLLF
                     VGDQITGVVDFEWAFAG"
BASE COUNT         2413 a         1690 c         1875 g         2364 t
ORIGIN      
        1 tattgctatt tccctcaact agttttttat tttcactggt aaatttgaaa tttattttgt
       61 taatttcaat gctgaaggtg taggggtttt ccacttcgat ttctgatctg aatttaatag
      121 caaaaggagt gaccccatca gggagtatgc ttacaatttt agcttttggt ttttccctga
      181 tgatttgcag gtaagaacaa gagataaaag tacaaaaaga aaaacagata aaaaatattt
      241 tagataatag attcatgaat ccccttgtgt cggtgagcaa gactgttaat agactacaat
      301 acaaaagaaa aatttcgaaa ttatttctat caataaagcc agccaaaagc gtcggtgact
      361 gttgctaaat tacaggcgga aagaaatact gccaaaaagt gagatcgcat aaaataccta
      421 ttatggaaaa atgagctgta gatgtctgaa atttttgagg aaggcggaat atggaaccag
      481 agaagtcgaa aaagcgaaga gcgaaaaaat taaggcaaaa cgagacccag gagattctga
      541 ctccagagtt tgatgagctt acggatcagg agttggttct acgcgatcag gaaatagctc
      601 tgcattctcc gcaggaaagt gattatacca ctctggaacc tccaagtggt tcagaacccg
      661 aaaaatcatt ttacacgctc acagataagg atcttcttga ggaggttgtt gaattgctag
      721 atcggcatcc ggagattgat gcgactgaaa ttaaagttca agtggagaaa ggggaagtca
      781 ttctgaccgg gggtgtgccg gaggagaaaa tgaagttatt atccgctgaa gtgataaagt
      841 tacttcctgg ggtgaaagtc gtcagcaatc agctaaggct tctcaatcca taaggtttat
      901 tttgtaccgg aagcagtttt ttcgtgtgcc gttggagttg gtgtctaaga agtttacaca
      961 tctgacttta caatcatgaa cttattttag agcttagaaa attttagaaa atattttcaa
     1021 atctaatata taaaggtttc cacaaattgc aaaattctat tgaaggagcc tttatgaaaa
     1081 aatcccttta ttctgccttg cttttaattg gaatttccct tgtgccctta tgtggctttg
     1141 cagggtttga tatgaatgca gttaaggatt tgtatcagcc gacccagcgc tcaaggcttt
     1201 actcaaaaga tccggcttat acccctaact atcgttttgg gattaaatat acattcatgc
     1261 cttaccagga tcccgatatg catgaggcca tcgacacgaa caatccactt ttgaatccag
     1321 cctatgactc ctataatttg attgttattg ttaataagtt tgaccgccgc gatcagtggg
     1381 gccgcgctca aaccatgaga gtttataagc gcaatgtaaa agaaaacgat ggtttgatat
     1441 attattggta tgtttccaca ggtatggacg gtttcggaac taacgcggga tatttcgttc
     1501 cgcaatcatt ttcttcaaga cactggtcca gtcaatatga tgctcccatg cgttcctccg
     1561 tgttttttct tggcggaaaa gcgcttcatg cttccatcga ttccgactct ttaaaagccc
     1621 ttggcactcc ttattcccat ggctgcgttc atgttgaaga caatagggca gaagaacttt
     1681 tccatctcat tggtcacagc ggctatggct cggtggatct tattgaccaa aaaacgggaa
     1741 agctaaagtt agaaaacggg cgagccataa aagtacaagc gactaaaact ctcattatag
     1801 tacattaagt ctaaaacgaa attctctgag ccatcatcgt gagggttcag agatcttatt
     1861 ttgaaacgct cccttaaatg cttggtcggc tttttaaggt atatgcattc tttttttgaa
     1921 gcaatccaat tttgattcaa atataaataa attatcattc ataagcattt aattcgggaa
     1981 ttttaatagt ttctcgatat actgagaacg cataaaccgc gccttttcgt tccgggggga
     2041 aaatgaaaaa aatctggttg aggtggttgc tgctgtgcct tttgggagcg ggaccaatgt
     2101 caattctgtt tcaaaattgt ggacctgcga agctcaccca aaaaaactca aaggagtctt
     2161 atacaagttt agggagcgat ggcaacccta cacctacacc tacgcctacg cctacgccta
     2221 cgcctacacc tacgcctacg cctacaccta cgcctacgcc tacgcctacg cctacgccta
     2281 cgcctacgcc tacgcctacg cctacgccgg caccgacacc gactcctgcc ccagggattt
     2341 cgattaatgt ggggggcacc tcagtgggga attttgtagt cgatcaatat ttctctggag
     2401 gcatgaccta cgcaaatgtg tcgggtgcca tcaatgtgga tacaagtggt gttttatatc
     2461 aggctccgca ggcggtctat cattgtgagc gatacggaaa tttttcttac acgattccag
     2521 gtttaacccc cggcgtagcc tacgaggtcc gtctccattt tgtagaagga tactggcaag
     2581 cggtgggtca gaggatcttt aatgtgtcaa taaatggcac caaggttctt tcaaatttcg
     2641 atatttttgc cactgcaggg agcatgaaca aagccgtact tcagacgttc ctgatggctc
     2701 caagttcgga tgggaccatg cttattaact tcacctcaat tatagacaat gccaaagttg
     2761 atgggattga ggttctatta aaacactaat tcatacggcc attaaatatc tgtgttcaac
     2821 aatggaatct tctgggagag ttcggtagtc aggagaagtg ggtaaggatg atgagtcatg
     2881 gatcaacgaa ggaaaaagat aaacatcgtc agtataggca atcgctgggt gatcaagtca
     2941 gcacaattga gcaggaagcc cgcatgttgc ttccgggaat acaaacctta tttggatttc
     3001 agctcatctc agtttttaat ccgggtttta aagcgagtct ttctgaggaa gagcaaatca
     3061 tacatctgat agcgctttta ctagtagcta tttcagcggt gttagtggtg gcgccggcgg
     3121 cttatcatcg ccaggcaaag catcaaatct cagaacattt tatagaactt tccagtaatt
     3181 ttttagcctg gtccatggcc cctgtggctc tgggcacttg cttggatatt tatttagtga
     3241 cgagggttat tttggtttct catttatata gttttataat tacgctgacg atttttactt
     3301 tttatgcttg gacttggttt gttctgccga aacggcgcgc gaaaaaaatt agatttttgc
     3361 caacccatga actgaacgaa gaaaaaaaat ctcacattta gccgcctccc atgtgttatt
     3421 ttgaaacgag ttgatcagaa aactacctaa atttgtttga ccgaattaaa ctgaggcaat
     3481 gagaaaactg acaagtcgaa tgggttctat tttaaaattg tcgctttgta tccttatggt
     3541 cattaactcc caagttcatg cttattctcc aaaggcgcca gtgcttgaag atgatttgga
     3601 cgcaagatcc actcttgtcc gcgaacgcct tttaattgaa tatgcagaaa ttaaaagcgc
     3661 ttcccagttt atagagaaat ttgcacctca gaggccggct gccgagaagg tggaactgtt
     3721 aaagcgtctt tcaaaactga aaagacttcc caagttagag gccattgcaa atggtctgag
     3781 gctcactcag gattcgatgt cttttgatat ggacctgcag tccatatcaa aacgacgttt
     3841 tcttatcaac ggtcaagtct tcacattctc accaaatcat tcttgggtga ttcaatcgga
     3901 aattttatgg gataaactcc aacatccgcg aaagtcaagc tacagtcttt tttcactttt
     3961 aattccagaa gcccatgggg ttgttatgca aaccattatg gcgtttggat atacagtgat
     4021 tcttatgggc ttagtgaata cagctattgg tcattatgga aatgatggct ttaatgtctt
     4081 ggattgggaa gtttgcgaaa gggtaaggaa atccaataac ggcgaatttc cgaggaatcc
     4141 tagattctgt aaggactata gaagagaggt ggaagagcag gcgaagaaga tgcctggcgc
     4201 taatgcggtt caagtcgcgc tgacggatgc tggctcaaaa agtgaaaaat attcgactag
     4261 taatgaaacg tgtcctgatc agaacaagga tcagcattac aatggaactg tgaccatcgt
     4321 ttctaaggag gaacccactc aaagaaataa ggatgttaaa aacgaggtcg tgaaatcaaa
     4381 cgaagggttg aagctagtaa gagtttacgc tgaagtggat gggacacgat tgaagagtgc
     4441 tagtgtctat catgatgacc ttgttaaggg tgaaagtaaa tcgaagttgc ttgcaaccta
     4501 tgtcgttgat ggtaactccg ttttagaaga aatcgtgata ccagcgagtt caaaaataaa
     4561 ttcgaagtta agtaaagaga agctaacgac cagaattagc gcatcagctg atcttaagga
     4621 taagccggat ttgcaggctc aacaggcgtt ttttcaaggc ttgttttcaa acttaggaga
     4681 tcgtcttaaa ttttgtaaaa tgcaaaaaga aaataaagag gcaaaagaag ccgttaaaaa
     4741 agtagaaaaa gggccccgtg aatcggcagg cactcccacc ggtggtaaca caggacattg
     4801 gtaaatttag atgtgaagaa gagtggaaaa gccttgatca ttaataaaaa ttctcatcaa
     4861 atgtccaaaa tatttaagtt tatccatggt ttggccagat taaaaaataa aaattaactt
     4921 tctgaacagc caataaaggc tacatccgaa ggactagatg aagttatgtg catatgtcag
     4981 atccctttta atgttttgcg taatattttt gttgggcgcc atgagctatg ctcatgagat
     5041 gaaggtaatg accttcaata ttcgttgtgg ttcttgtgag gactcttcca gcattaatca
     5101 ctggagtaaa agaaaatctc ttgtgaggga gcttattaag agtcaggcgc cggatattat
     5161 tggtcttcaa gaggctgaac agtttcaaat aaaagattta gctaatttgt tgggtgacta
     5221 tgattggatt ggtgtggggc gtgacgatgg aaaggaggct ggtgaggcca cagtgatttt
     5281 ctttcgcaaa agtcggttct cgcttctcac tcaaaagact ctttggcttt ctgacacgcc
     5341 ggaagtagtc tcacgtgggt gggatggaaa actcaatcga acggtgacga ttttaaaact
     5401 taaggacaat aattctaatg gacgagagtt ttatttctac aacacgcatc ttgaccacgt
     5461 tggacagcag gctagagccg agggattgca actcatagta aggttgatga attcgggctc
     5521 tgaaaatttg cctctcattt tgacagggga cttcaatttt acccgcgact tttgggctta
     5581 caatattatt gcttcccgat tgctagatgc tcagtttatt tctcaatctc ctcccgaggg
     5641 cggcaatcag acatttaatg gattcggcag ttcgacagat ccaaataata aaatcgatta
     5701 catttttgtg agcgattcat ggaatgttat ttctcatcga atcagtactc aactttacga
     5761 gggacgttat ccttctgatc atttccctgt agaagcgcgc ctcactctca aataatttat
     5821 tgaggggctt accggaatct taagctcttt ttcaggcaaa attcctaatt ttcttgggag
     5881 actcctggtt tgtatccttt ttttgaaatt ttgatggctt tttaagcact tatcatacaa
     5941 ataaagagac tttggtagtt gacgtgcgaa aggctgaggc ctatattggc gcttcattca
     6001 aatatctctt ttgtcgcggg gtggagcagt ctggtagctc gtcgggctca taacccgaag
     6061 gtcgtaggtt caaatcctgc ccccgcaacc aattcttccg aattggagtt caataaaatc
     6121 aaaggcttag gccaatttct ccggaacctc aggttccacc ggttcacagc aaaaaatcag
     6181 atcagaaaaa taaaatcaag cgatccatac tccccggcgg gtgagcagga ggcagacgca
     6241 cgtcctcttt atgggatacg tgtgccgtga aaagcgaccg caaatattgt agggtcagct
     6301 cgacaaaata tataggagtt aagtttatga aggaaatctt agaaaccttg aaaaaggctt
     6361 gtggcgttta tcttggagaa ggaatcaatc acgaaggcca accttttaca ggccgtctta
     6421 gcttagaacc gcttctcgcc ggtcgcggct ttcaaattca gttctccgca actggaaaag
     6481 acggaaagct ctatcaccaa gaggcatcca ctattgcgcc ggcaatgacc gagaagttga
     6541 ccctatggaa ttttaataca aatattccgg gccttgttcc tcatgaacta cggtcagcaa
     6601 tagcgaagcc tagcgcgatc agctccctcg tctttggatt taactcccct caggacgaca
     6661 aagcatttcg agaagaagtc gctcttgata tttgggatag caattcaatc tcttacactt
     6721 attcatgggg aatgcctggt ggcgagttca aggaacgctc tggagttcgc atggtcaaac
     6781 aaactgaaaa accatgaata cgtcgattga aacttttctt caagattttc ataaaaaaca
     6841 tccgggttgc actccggctg cattttcaaa tggattcacc aaggaccata aaacatctta
     6901 tgatatggtt gcctccgtac tcgccccatc aagcgaaaac caagcaatcg ttttagatct
     6961 ggcttgcggc gatggagtcc tacttcaaag gatttctacg gccggatata aaaatctcaa
     7021 cttgatcggc gtggatatga gtgttggcga gctcgaagcc gctcgcacaa gacttagctc
     7081 ggcaaaagtc aaactcttgg aagccaaggc gcaatccctt ccactaccag ataactcagt
     7141 cgactttgtc ctctgccata tggcctttat gttgatggat ggaattgaaa atgttgtttc
     7201 tgaaatccat cgtgtcctca aacctggcgg aaccttctcc gcggttgttg gcggaaagta
     7261 cgagaaagct ccggcgatgg acgccttcct tcaattgttg gatgaggccc tcaaagaaga
     7321 gagtcaatct tggctttcaa accttggcga caagaggaca cgaagtgaag acggtctgcg
     7381 gtctctcttt acgaaagatc gatttgtcca gtcaggcacc gttcaggact tctcgattcg
     7441 ctttcatgat cgaccagaaa atctcatgga ttttttcatg ctcatgtacg atgtggccgc
     7501 attgtcacca gcacgccaag ttaaacttgg cggcgaccta ttattaaaac tcgaatcgct
     7561 cagtgatgag atgggaaaaa tgcaccactc tatggggttg cgccaaatca tcttttgcaa
     7621 gggggatagt taagtcatga ggacacctga ccatctcaag attaaggaaa cttgtcgtct
     7681 tgcgatactg ccgctgatga caaagcacgg cctgggatcg ccgatcaata ttctggttga
     7741 cgagactggc tgggtgaacc cgtgcttctt tgtaaatgat aaatttgttt ttcggtttaa
     7801 tgcgcgggat cctgagctgc caaaatatca gcgagaaaaa tttgccttcg atcttcttaa
     7861 aacttcgacg gtcccggttc ctcaaaaggt catattggac gaaactaaaa catcggcccc
     7921 attcgatgtt ctcatcacgg aaatgattcc cggagagaac ttagaaactg cttggccaaa
     7981 attaaatact gaacaacggg aaaatctagc tgaaaatgcg ggaaaactgt tgaaacaaat
     8041 gaccacaatg cagttgccac tctttggtga actggcctct acgggtccgt ttccgaagac
     8101 cgaaacatgg gtaagctatc ttcaagcgaa actttcattc catttaggcg aggcccttag
     8161 tctcgaaatc ttcgatcgca aggttattga gaaattttgg aacactttta ataagcacgt
     8221 ctctgcactc tcggaagtca gcatccctcg ccttgtccat gtcgatttcc actttggaaa
     8281 tcttttattt gttggcgatc agattacagg agttgtcgat tttgagtggg cttttgcggg
     8341 ag
//
LOCUS       DATBTE010000131         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1997965, whole genome shotgun sequence.
ACCESSION   DATBTE010000131 DATBTE010000000
VERSION     DATBTE010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997965"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VIG33_04895"
     CDS             <1..420
                     /locus_tag="VIG33_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8270202.1"
                     /translation="RPLSTKRPIHLVLKSTGSSFFNPGNRHLEKILRDHAQRYSIKLY
                     ALTLNWSHIHLLIKLPTRGSYLAFIRTVTAAMIKYLSKQKGKSLKGIFDLRPFTRILS
                     WGRDFQNAFDYQKLNELEGWGVICRKKKSPQKQKESS"
     gene            534..1250
                     /locus_tag="VIG33_04900"
     CDS             534..1250
                     /locus_tag="VIG33_04900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8270203.1"
                     /translation="MPPQPQLSLCVPLCSEKELIQEFHENLSHFFQRIPLVYEVLFAV
                     NPGQSESLFLLQKLIEKNPQYRILENKKNFKNKFSRAQNIQMLCANARGSILIPIDLD
                     LVVPLSEIFKILEAFFSDTELEVVFGNRFKAKKNIENQNLEQDRLEKFFMEILKEKTP
                     WKFADPFCPMLGLRRTSFEKIEKELKSSGWHWNHEVQRLVQSKGLKSQEIPLYVGVRG
                     RLKIPKSEIFHLLNFVLFRI"
     gene            complement(1221..>2190)
                     /locus_tag="VIG33_04905"
     CDS             complement(1221..>2190)
                     /locus_tag="VIG33_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HEY8270204.1"
                     /translation="GRPANFSGAVGQFDVQANVDGQTILVNQPFSLKVRFEGLGNAKL
                     IELPAIEWPKTVEVYDTKSEAKFFKNGRSFKEFEILLIPRQEGELKIPVISVGTFDPQ
                     AKKYVLRQTQELTLRVDPNPNAPSGNIGGSGNVAATPQAPAKANSLSGHVLPDLILTW
                     EEPSKFNIASQSSFWGGIYAFVILLLVWRSKVELGWGRRRQNLRELVNKRYQKLETLL
                     RKNDYRGIGSEMMNIFNLVLGVISGQEASGLELDRMLERLSPSLRRDHGEAVKKQYEI
                     FQVMSFAPEDMLGVYKQESQVQKEVAAAKDLLQKLIASNPEQNEVQ"
BASE COUNT          631 a          507 c          428 g          624 t
ORIGIN      
        1 cggcctcttt ccacgaaaag acctatccat ttagtgctaa aaagcacagg aagttctttc
       61 tttaatcctg gcaatcgaca tcttgaaaag attttaagag atcatgctca aaggtattcc
      121 attaagctct acgctctcac cctcaactgg agccatattc atttactcat caagcttccc
      181 actcgaggaa gctacttggc ttttattaga acggtcaccg ctgcgatgat taaatatctt
      241 tcaaagcaaa aaggaaaaag ccttaaaggg atctttgacc ttcgcccttt tacacggatt
      301 ctcagttggg gccgggactt tcaaaatgct tttgattatc agaaactcaa tgagcttgaa
      361 ggttggggag ttatttgcag gaaaaagaaa tccccccaga aacaaaagga aagcagttag
      421 caactgtttt ttgtttttca gccccctgtg cctgattgga gggtgaaatc cttcacacca
      481 gtggcttttt atagtatcct agcataggat tctctgaata gaggaaaagt taaatgcccc
      541 cacagcctca actctcttta tgtgttcctc tgtgctctga aaaagagctt attcaggaat
      601 ttcatgaaaa tctaagtcat ttttttcaaa gaattccttt agtctatgaa gtgctctttg
      661 ctgtgaatcc aggtcaaagc gaaagcctgt tcctattgca aaagctgatt gaaaaaaatc
      721 ctcagtaccg catcctggaa aataagaaaa attttaaaaa taaattcagt cgagcgcaaa
      781 acattcagat gttgtgcgca aatgccagag gaagcatttt gattcccatc gatcttgatc
      841 tagtcgttcc tctcagtgaa atatttaaaa tccttgaagc ttttttctcc gacactgaac
      901 ttgaagttgt ttttggaaac cgcttcaagg ccaagaaaaa tatcgaaaac caaaacctcg
      961 aacaagacag actcgaaaaa ttttttatgg aaattcttaa agaaaaaact ccctggaaat
     1021 ttgccgaccc cttttgcccg atgttgggac tgcgtcggac cagctttgaa aagattgaaa
     1081 aagaactcaa gagttcaggc tggcattgga accacgaagt tcaaagactt gtccaaagca
     1141 agggactcaa atctcaagag attcccttgt atgtgggagt tcgtgggcgt ttaaagattc
     1201 ccaaatccga gatcttccat ttattgaact tcgttctgtt caggatttga agcaattaat
     1261 ttttgcaata aatctttggc cgctgccact tctttttgga cctggctttc ttgcttataa
     1321 acacccagca tgtcttctgg cgcaaagctc ataacttgaa aaatctcgta ttgctttttc
     1381 acagcctccc catgatcgcg gcgaaggctt ggcgaaaggc gctctagcat tctgtccaat
     1441 tcaagacctg aggcttcttg ccccgaaatg actcctaaaa ccagattaaa gatattcatc
     1501 atctcagagc cgattccccg atagtcgttc tttctaagca gagtctctag cttttgatag
     1561 cgcttattca cgagttccct taaattttga cggcgacgtc cccagccgag ctcgaccttt
     1621 gatctccata ctaacaataa aatcacaaaa gcatagatcc cgccccaaaa ggaggattga
     1681 gaagcgatat tgaacttact gggttcttcc cacgtcagaa ttaaatccgg taaaacatgg
     1741 ccacttaaag agttcgcttt cgcaggtgct tgtggggttg ctgccacatt gcccgagccc
     1801 cctatatttc ccgaaggggc attggggttc ggatccacgc gcaaggtcaa ttcctgagtc
     1861 tgacgaagga catatttttt agcttgagga tcaaaagttc ccacgctgat cacgggaatt
     1921 tttagctccc cttcctgacg agggatcaaa agaatttcaa attctttaaa actgcgacca
     1981 ttcttgaaaa actttgcctc cgatttggta tcgtaaactt ccacagtctt tggccattca
     2041 atagctggta actcaatcag cttcgcattg cccaaacctt caaaacgcac tttaaggctg
     2101 aagggttgat ttactaaaat cgtctgtccg tcgacattgg cttgaacgtc aaactgtccc
     2161 accgcccctg agaaattagc gggacgtcct
//
LOCUS       DATBTE010000132        10291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1520896, whole genome shotgun sequence.
ACCESSION   DATBTE010000132 DATBTE010000000
VERSION     DATBTE010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 10291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10291
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520896"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VIG33_04910"
     CDS             complement(<1..230)
                     /locus_tag="VIG33_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270205.1"
                     /translation="MEYQRSQTRRGHKSKAGRPFYLTKRDQQILEFIWKWKIAPTSVI
                     YEACVKPNSTQYSAYKIMEKLEKNGYVEAKFN"
     gene            complement(257..1627)
                     /locus_tag="VIG33_04915"
     CDS             complement(257..1627)
                     /locus_tag="VIG33_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEY8270206.1"
                     /translation="MSEQQNEQFWKEKLNNIARISWFAIAELIQGFRKGTIPIFTYCL
                     PGLFVALCLISKFDYFAADVLGKSSLRLTRSTSLIATYLSLFSGFILWAFQRAHQRNY
                     ALSKLKEAFERAQLNHGAKFPSLIDDIAVDDYVRRLKIKNNGQPIDKWIAVKEMIATN
                     LNISIIKMNYYEEDRSRIEIIYSLKPMLRSYPFPTDLKFPEGDIPIGVSYGGPIIVNL
                     HRLPHMLVAGETNCGKTNFLKTLSRVLVENNENCEVHFLDYKESAEGLSLLSSFGGAH
                     DRIHVFSNKEANTKHIVSLATTMDARLKELSELGAVNFDEYLRKRISVTKKSGKGDPT
                     RQEKLRRMYIVIDEIAEVYAKGKDLSKGLADEARAALNRLTRQARSAGMHLVICTQYP
                     SAQGLDTTVKNNLPGVLCFAQPNVAGSTAAIGNNRATMIDPDIKGRAIWKVGPKQEEV
                     QTYLFQ"
     gene            complement(1633..2532)
                     /locus_tag="VIG33_04920"
     CDS             complement(1633..2532)
                     /locus_tag="VIG33_04920"
                     /inference="COORDINATES: protein motif:HMM:NF015688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HEY8270207.1"
                     /translation="MSEEIKQAEVTIETDEKTLPWGSGGYFKTQDGLKEKIGGLKLIF
                     YAATIIVGVFGLFARRDQKKDTRGTAMSSPTISSQVPMAVPDIKTKTEEVNYRRQSRL
                     EESHLGKIKVFNLRGSGEIPTGSEARAILESGATNGIVKGRLTSPLLINDDPLLPEGT
                     IIFGKGKSTEERLYVEFHKAILPSGEVLAIRAQAFDESDKIIGLKGSAVGTKTKKMAM
                     GMGFGFIGGLAEGLQEDTSGTIFGLQRKRSMRDAALGGASKAALGQSQAMIDEIKNSQ
                     DIIEVKNGTEFFIIIDDPQEKEK"
     gene            complement(2525..2908)
                     /locus_tag="VIG33_04925"
     CDS             complement(2525..2908)
                     /locus_tag="VIG33_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270208.1"
                     /translation="MKWFMAILITFVILWTAEQAMSGKTKEKTIECDDKKSKTVRVVF
                     GRVTVLNFPFKPSDVIPGKMNFDFKRIKNDLIITALRPGSKTNAIVYLEGRRCSFDLI
                     TVSSSGDDILTVQDPKDSRYEVNFE"
     gene            complement(2905..3447)
                     /locus_tag="VIG33_04930"
     CDS             complement(2905..3447)
                     /locus_tag="VIG33_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270209.1"
                     /translation="MRFSSTMDSLTKEIQFLKKVVLGLILVAGCLTVAVMVLFEKEPL
                     IVERSVKGLEILKIASFERKEEDLSQALKIMAKARFDSNALSPELFLSSRQLELREAE
                     QKEMKARSMSQAIVVRSIKITKNEALIEFDRVLSLGTVRSALATQVKVAFEESVPNEL
                     NPYGLKLAIVSPVDSKEVGK"
     gene            complement(3485..4345)
                     /locus_tag="VIG33_04935"
     CDS             complement(3485..4345)
                     /locus_tag="VIG33_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270210.1"
                     /translation="MDMHWLAQEAKQVHDVFAGLFYSLVLSLVLIGVVINFFKFPMGE
                     VPEFFQLAGRAIIAAFLLVAFPEIMNTLSDITDQISKEIGQLNNFSLVLHRLGEKIGD
                     LTWSWVSFKDSILLLISYATFFILYITVYFADAMFLMTWTLLYVFSPILIAAFTLPST
                     SKATIGLFQSLIEVCLWKICWSVLATLLWSFALSQINKPEYDVDFLTAILLNIMLALS
                     VLITPMVVRSLIKGGVHSTAAIAGGAILSVASLTPSGAVGAATSGIVGGAKSIASPKD
                     EGHERGIPHS"
     gene            complement(4346..5035)
                     /locus_tag="VIG33_04940"
     CDS             complement(4346..5035)
                     /locus_tag="VIG33_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270211.1"
                     /translation="MKKTLATILVMASLFCPLRSARADLFGGDVAVLTQILANAIQQL
                     AQLREILDNGEDSLNLLRDINRGINDSLLMAETFGIRVDPGIYRDLRKVDQTVLSVEA
                     IFGKPVDSPLAPVQRNTDQTIAESISFNNELNDYTLKLDKIGEQIKDYSHAVSPGGAQ
                     KLTAESLGVLIHVMNQQIRATGQGLKLQAQALAVQNKQEKDRSAQYLKEGQRLKQNMT
                     SLQANFEAPRF"
     gene            complement(5052..7451)
                     /locus_tag="VIG33_04945"
     CDS             complement(5052..7451)
                     /locus_tag="VIG33_04945"
                     /inference="COORDINATES: protein motif:HMM:NF040179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8270212.1"
                     /translation="MNQLAEKLEVWGFEEGLLIYKDLSLGVVLKLSPKDISCVTDDLL
                     NTLHGAIGNFLNGLPAKLNLQFVQEISKGASRILEAHQKGLKPEALEIVGNMLTERTQ
                     RLAAFDKAGAIPAQNIYLVIRKSFDRRGQRKKRFRFWEKKDEEQDWSREILTPELKSM
                     NQIVETVQKGLEALGVQSLKLPEAETFDLLFEQWNPGHPLPGLNFNREDIRDELILND
                     VVVSIKGFSIAQHYHRVLSVKIMPEQTFSAMAEKLKELPFGSKLFLTIETLDQDKEDL
                     ALQTQRRVAYAMYSGKKGVSDLESKAKLEDIETLLQKRVSGEEKIFSAALNVVLRSTS
                     ESELEEQVQNVLEKIRALSGAEGMVESLAAASIFFDLALPNARAKERSRKMNTSVLAD
                     FLPLYGDWRGHAEARVLLRNRSGGVLGFNPFSPTHGNFNQIVSGGSGAGKSFANVLMV
                     SHLMKEDPKVFIIDIGASYKRLSENLDGQYIGIGTDSSLSLNPFDLSDQSSEALDQKI
                     KFMASLVELMTKEDDSTGIGKLERSEIEQTIKEIYREEKDPKLSHLRERLLKNNEPLL
                     QRMGKILGTWCGDSPYGKFVDQKTNLSLKKNIVCFDLKGLESQPDLQAVCLFIIMDLI
                     WREVQKDQTNMKIVIFDECWSLLENEAGSRFLGEVYRTFRKYRASAIAISQTMDDFAN
                     SKVASAILPNSSVKWILKQTGGNIPSLQKILRLNERETKLIESLTSKKGYFSEAFLIS
                     GDDRQIVKIESTPLEYWLATTDPLDLKAIEEMKQEHPELTGTHLLKNLSERFPRGASN
                     P"
     gene            complement(7455..7775)
                     /locus_tag="VIG33_04950"
     CDS             complement(7455..7775)
                     /locus_tag="VIG33_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270213.1"
                     /translation="MALKTSKVYRCLEKKTLVFGFEIVDLFLVFSILAVLNLLFGSMP
                     YKFFFTWLPSISLAAFLRISKAGKPDNYLVHFARYHFSPGIFSAFPLATQRMKFVTRT
                     KGAK"
     gene            complement(7788..8114)
                     /locus_tag="VIG33_04955"
     CDS             complement(7788..8114)
                     /locus_tag="VIG33_04955"
                     /inference="COORDINATES: protein motif:HMM:NF016820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HEY8270214.1"
                     /translation="MKNFVITKVKNIQERTGTLANKFLLPLAVVLTTAPFAQASVESS
                     LSAVQDKLVGTILPLAAIVGLSWAGLSFVIGHPQARQRMVYALIGAGVGFGAESLISL
                     VRSLIH"
     gene            complement(8178..8483)
                     /locus_tag="VIG33_04960"
     CDS             complement(8178..8483)
                     /locus_tag="VIG33_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270215.1"
                     /translation="MCLKNEYLVQEIEEFLESISPGVDADELHEAIEEAKNLLEDESD
                     AETIRNHLENLWDLIPMIEQKELEDTFSNIMNALESEMFGGGDDDNYFEDESEDDDL"
     gene            complement(8474..9241)
                     /locus_tag="VIG33_04965"
     CDS             complement(8474..9241)
                     /locus_tag="VIG33_04965"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEY8270216.1"
                     /translation="MQKQLPKPWLTSGGVEIPTEQIMNLCQNWDPPTWERYLKWYESG
                     RREALVLATLYSEKCEQMEESIFSQFGYHTSPEKTQLCEDLLNELPKMERRVLSLVFL
                     NGKTEREIAAEVNRSQPGVHQIKNRALLRLQRGIQGNEVITRRYMRGEDSSIEAPLSN
                     YAREEKVYDPKNYKEELAEIKHRELSQAVRELSERQQQIIYLRFWCDYSFKQIARDLG
                     IGVNFAEQICDAAVSKLKIKVTEMSTTCETDGGPSCA"
     gene            complement(9925..>10291)
                     /locus_tag="VIG33_04970"
     CDS             complement(9925..>10291)
                     /locus_tag="VIG33_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270217.1"
                     /translation="DNVQVKLKRAPDVDIYLDHDENDITTRSYFSQNKLPFKPLTMRY
                     LDDVYGLIDGVKNGYGKAIVPLHLIEHESTLEILEPKRVLRVSVYLQFFNQPYHRKVH
                     SAFIDEVQTFFKEKLRQEK"
BASE COUNT         2674 a         2261 c         2296 g         3060 t
ORIGIN      
        1 tcattgaact tagcttcgac gtatccgttc ttttcaagtt tttccatgat cttataagct
       61 gagtactgag tcgagttagg cttgacgcaa gcttcataaa ttacggaagt tggagcgatc
      121 ttccacttcc agatgaactc aagaatttgt tggtctcttt tggttagata aaaaggccga
      181 cctgcctttg acttatgacc tcggcgtgtt tgactgcgtt gatattccat agatacctct
      241 cataaaaaat tgaaatttat tggaagaggt atgtttgcac ctcttcttgt tttggtccga
      301 ctttccaaat agcccggccc ttaatgtcgg gatcaatcat ggttgcgcgg ttgttgccaa
      361 tagcagcagt gctcccggcc acgttgggct gggcgaagca cagcactcca ggtaaattat
      421 ttttgacagt ggtatccagt ccctgagcgc ttgggtattg ggtgcagatc acaagatgca
      481 ttccagcgga cctggcttga cgggtgagcc gatttaaagc ggctctcgct tcgtcagcta
      541 agcctttgga aagatctttt cctttggcgt agacctcagc gatctcgtca atcactatgt
      601 acattcggcg aagtttttcc tggcgcgtgg gatcaccttt gccggacttc ttggtcactg
      661 aaatcctctt tctcaaatac tcgtcaaagt tgactgcacc taattctgaa agctctttca
      721 atcgtgcgtc catggtcgtt gctaaggaca ctatatgttt cgtgtttgct tccttatttg
      781 agaatacatg gatgcggtca tgggcgccgc caaagcttga aagaagtgat agaccttcgg
      841 cggactcctt gtaatcaagg aagtggactt cgcaattttc gttattttca actaatactc
      901 tggacagagt tttcaaaaaa ttggttttac cgcaattggt ttcgccagcg acgagcatgt
      961 gagggagcct atgcagattc acaatgatag gtcctccata ggacacacca ataggaatat
     1021 caccctctgg gaacttgaga tccgtcggga atggatagga tcgcagcatg ggttttagcg
     1081 agtagattat ttcaatccgg cttcggtcct cttcgtaata attcattttg atgatactga
     1141 tgttgagatt tgtggcgatc atctctttaa cggcaatcca tttgtctatg ggctgtccgt
     1201 tatttttgat tttaagacgc cttacataat cgtcgacggc gatgtcatca atcagtgacg
     1261 gaaactttgc cccatgattg agttgggccc gctcgaaagc ctctttaagc ttactcaggg
     1321 catagtttct ttgatgggcc ctttgaaacg cccacaaaat aaatcctgag aagagactta
     1381 agtaagtcgc aatcaagctt gttgagcggg taagtcttag agaagattta ccaagaacat
     1441 cggcggcaaa gtaatcgaat tttgaaatta gacacaaagc cacaaaaagt cctggaagac
     1501 agtacgtgaa aataggaatc gtgccttttc gaaatccttg aataagctct gcgatcgcga
     1561 accatgagat tcgtgcgatg ttgttcaatt tttctttcca aaattgctcg ttttgttgtt
     1621 ctgacattat ctttacttct ccttttcttg aggatcatca atgattatga aaaactcggt
     1681 cccatttttt acttcgatga tgtcttgaga gtttttaatt tcatcaatca ttgcttggct
     1741 ttgaccaaga gctgccttgg aggcaccgcc gagggcggcg tctcgcatgg atcttttgcg
     1801 ttgaagacca aaaatagttc cgctggtatc ttcttgaagt ccctcggcca gtccgccgat
     1861 aaatccaaag cccattccca tggccatctt cttagtttta gttccaaccg cagagccttt
     1921 gagtcctata attttatcag actcatcaaa agcctgggct cgaattgcta gaacctcgcc
     1981 tgatggaaga atagctttat gaaactccac ataaagtcgt tcttcggtgg acttgccttt
     2041 gccaaaaatg atagtccctt caggcaaaag cggatcatca tttattaaca gcggtgatgt
     2101 aagtctccct ttgacgattc catttgtggc accgctttca agaattgcac gggcttcgct
     2161 gcctgtggga atttcaccag agccacgaag attaaagact ttaatttttc ccaaatggga
     2221 ttcttcaaga cgtgattgac gacgataatt aacctcctca gttttagttt taatatcagg
     2281 tacagccatt ggaacttgag aggaaatagt cggtgaggac attgctgttc cacgggtgtc
     2341 ttttttttga tcccgacgcg caaaaagacc aaaaacacca acgataatgg tggctgcata
     2401 aaaaatcagc tttaagcccc caattttttc tttaagtccg tcctgggttt taaagtatcc
     2461 gccactcccc cagggcaggg ttttttcgtc agtctcaatc gtgacttcag cttgtttgat
     2521 ttcttcactc aaaattcacc tcatagcgtg agtcttttgg atcttgcact gtcagaatgt
     2581 catctccgga gctgcttacg gtaatgagat caaaagagca gcgtcggcct tctaaataaa
     2641 caatggcatt ggtctttgag cctggtctga gtgctgtaat gatgagatca ttcttaatgc
     2701 gtttaaaatc gaagttcatc tttcctggaa tgacatcgct gggtttaaag ggaaaattta
     2761 agacggtgac cctaccgaag accactcgaa cggttttcga ttttttatca tcacattcga
     2821 tggttttttc ttttgttttt ccggacatag cctgttcagc ggtccataaa attacgaatg
     2881 taatgaggat tgccataaac catttcattt accgacctcc ttcgaatcga ctggtgacac
     2941 aatggcgagt ttgagcccgt agggattgag ttcatttggg acagactcct cgaaggccac
     3001 tttgacttga gttgctaagg cggatctcac ggttccaagg gaaagaaccc gatcaaactc
     3061 gatcagagcc tcgttcttgg ttattttaat cgacctcacg acaatggcct gagacatcga
     3121 tcgagctttc atttcttttt gttctgcttc tcgtagctcc agttgccgcg atgataaaaa
     3181 tagctcaggt gatagggcat tactatcaaa gcgggccttt gccatgattt ttaaggcctg
     3241 tgaaaggtcc tcttccttac gctcaaaact tgcgatcttt agaatttcca acccttttac
     3301 tgagcgttcg acgataaggg gttctttttc aaatagaacc atgaccgcca cggtgagaca
     3361 gcccgccact agaataagac ctaaaactac tttttttaga aattggatct cttttgttaa
     3421 agagtccatt gtcgatgaaa acctcatcag gaacctcctt caataaatta gaaataaaac
     3481 gattttatga gtggggaatg ccgcgttcat ggccttcgtc tttggggctg gcaatacttt
     3541 tggctccgcc gacaattccg gatgtcgctg ctccgacggc gccactaggg gtaagacttg
     3601 ccacggaaag aattgctccg cctgcaattg ctgccgtcga atggactccg cctttgatga
     3661 gactgcgtac aaccatcgga gttatcaaca ctgacaaagc aagcatgatg ttgagtaaaa
     3721 ttgctgttag aaaatccacg tcatactcag gtttgttgat ttgtgaaagt gcaaaactcc
     3781 aaagcagggt tgcaaggacg ctccaacata ttttccataa gcagacctct atcaaagatt
     3841 gaaaaagtcc aatggtggct ttcgacgttg acggcagcgt gaatgcggca atcaaaatcg
     3901 gtgaaaaaac atagagcagt gtccaggtca tcaaaaacat cgcatcggca aaatacaccg
     3961 taatatagag tataaaaaag gtggcatagc taatcaggag aagtattgaa tccttaaagc
     4021 tcacccacga ccatgttaga tcgccaattt tttctcctag ccggtgaaga accaagctaa
     4081 aattattaag ttgtccgatc tcctttgaaa tttgatcggt gatgtcggaa agcgtgttca
     4141 taatttcagg gaaggccacc agtagaaagg ctgcaataat agcgcgacct gccagttgaa
     4201 aaaactctgg aacttcgccc atgggaaatt taaaaaaatt gataacgacg ccaatcagaa
     4261 ctaaagacaa taccagtgaa taaaaaagtc cggcaaatac gtcgtgaact tgttttgctt
     4321 cctgggccaa ccaatgcata tccatttaaa acctcggtgc ttcaaagtta gcttgaaggc
     4381 ttgtcatgtt ttgtttgagt cgttgcccct cctttaaata ttgagccgag cgatctttct
     4441 cttgtttatt ttgaacggca agggcttgag cttggagttt taagccttga cccgtggctc
     4501 tgatttgttg attcatgaca tgaatcaaca cgcctaaaga ttcggcggtg agtttttgag
     4561 cacccccagg cgatacagca tgagaataat ctttgatttg ctcgccaatc ttatcaagtt
     4621 tcagggtgta atcgttgagc tcattattga aagaaatgga ttcagctatg gtttgatccg
     4681 tatttctttg aacaggtgcc aggggcgaat cgacgggctt tccaaaaatg gcttcaacgc
     4741 ttaaaaccgt ttggtcaacc tttcgtaaat cgcgatatat tccggggtcc acccgaatcc
     4801 caaaggtttc agccataagg agggaatcgt tgattccgcg attgatatca cgaaggagat
     4861 tcaagctgtc ttcgccgtta tcgagaattt ctcgaagttg tgctaattgt tgaatggcat
     4921 tggctaaaat ttgagtcaga accgcgacgt cacccccaaa gaggtcggcg cgggccgacc
     4981 tcagagggca aaaaagggac gccatgacca gaatggtcgc taaagtcttt ttcatagtgt
     5041 tcgtcctttt gttatgggtt agaggcccct cgtggaaatc tttctgataa gtttttcaga
     5101 agatgagtgc ctgttagttc tgggtgctcc tgcttcatct cttcaatggc tttgagatct
     5161 agaggatcag tagtcgcaag ccagtattcc aggggagtgc tctcgatttt aacaatctgt
     5221 cggtcatcgc cagaaatcag aaaagcttcg gaaaaatatc ccttctttga ggtgaggctt
     5281 tcaatgagct tagtctcccg ctcgttgagg cgcaggatct tttgtaaaga cggaatattt
     5341 cctccggttt gctttaagat ccatttcacg ctggagttcg gtaagattgc ggatgccact
     5401 ttggaattgg caaagtcatc catggtttgc gagatcgcaa tggccgaagc ccggtatttc
     5461 cggaaggtgc gatagacttc tcctaaaaac cgacttccgg cttcgttttc aagcaaagac
     5521 cagcactcat caaaaatcac tatcttcata ttggtttggt ccttttggac ttctcgccag
     5581 ataaggtcca taatgatgaa gagacacaca gcctgtaaat ccggctgaga ttcaaggccc
     5641 tttaaatcaa aacagactat attttttttg agggacaaat ttgttttctg atcgacaaac
     5701 ttcccgtaag gcgagtctcc gcaccaagtg cctaagatct tccccattct ttgtaacaga
     5761 ggctcgttat ttttaagcag tctttctcgc aggtgagaca gctttgggtc cttttcttcc
     5821 cggtatattt ctttgatagt ctgttcaatt tcggagcgct ctagctttcc gattcctgtt
     5881 gagtcatcct cctttgtcat gagttcaacg agggatgcca tgaattttat tttttgatcc
     5941 aaggcttcag agctttgatc agacaaatca aatgggttta ggcttaatga ggagtccgtg
     6001 ccaattccga tatactgtcc atcgagattt tctgagaggc gcttgtagct cgcaccaata
     6061 tcgatgataa aaaccttggg gtcttctttc attaggtgag aaaccattaa gacattggcg
     6121 aagctctttc cggcgccact tcctccgctc acgatttgat taaaattccc atgagtgggg
     6181 gagaacggat taaaacccaa gaccccgccg gatcgatttc gtaaaagaac tcgggcttca
     6241 gcatggcctc tccaatctcc ataaagtggc aaaaagtcag caagcacgct cgtattcatt
     6301 ttgcggctgc gttctttcgc tcgggcattg ggaagagcca gatcaaagaa tatagaggcg
     6361 gcggctaagg attcgaccat tccctcggct ccagaaagtg cgcggatctt ttcaagaaca
     6421 ttttgaactt gttcttccag ttcactctca gaggttgatc tcagaacgac attgagagcg
     6481 gctgaaaata ttttttcttc tccactgacc cgtttttgta gcagagtctc aatatcttcg
     6541 agcttcgctt tactctcgag atccgacaca ccttttttac cggaatacat ggcataggct
     6601 acgcgccgct gtgtttgtag cgccagatcc tccttatcct gatccaaagt ttcaatggtt
     6661 aaaaataact ttgatccaaa gggaagctct ttgagctttt ccgccatggc tgaaaacgtc
     6721 tgttcgggca taatcttgac cgacaaaacc cggtgataat gttgcgctat tgagaaaccc
     6781 ttaattgaaa ccaccacatc atttaaaatg agttcgtcac gaatatcctc gcggttgaaa
     6841 tttaaaccgg ggagggggtg accgggattc cattgctcaa agaggagatc gaaagtttcg
     6901 gcctccggca acttcagact ttgtacgcct aaggcctcaa gccctttttg cacggtctca
     6961 acgatctgat tcatagactt gagttcaggt gttaggatct cccgcgacca atcctgttct
     7021 tcatctttct tttcccaaaa acggaatctc tttttacgtt gtcctctgcg atcgaagctc
     7081 tttcttatta ccaggtaaat attttgcgcc ggaatagcgc cagccttgtc aaacgccgcc
     7141 agacgttggg tcctttcagt cagcatatta ccgacgattt caagagcttc tggcttcagc
     7201 cccttttgat gagcttccaa aatgcggctt gcaccttttg aaatttcctg aacaaattga
     7261 aggtttagtt ttgcgggaag cccatttaag aaatttccaa tagctccatg caaagtattt
     7321 aacaggtcat ccgtcacgca ggaaatgtct ttgggactga gtttcagaac tacccccaat
     7381 gaaagatctt tataaattag cagtccctct tcaaatcccc agacttccag tttttcggcg
     7441 agttgattca ttttctactt tgctcctttc gttcttgtga caaacttcat tctttgtgtg
     7501 gccagtggga aggcgctgaa aattcctggt gaaaagtgat agcgtgcgaa atggaccagg
     7561 tagttgtcag gttttccggc tttgctaatt cgaagaaagg ccgcgagact gatacttgga
     7621 agccaggtga aaaaaaactt atagggcata cttccaaaga gcaggtttaa aaccgcgaga
     7681 atcgaaaata cgagaaatag atccacgatc tcaaacccga acactaaagt ttttttctca
     7741 aggcagcgat aaacctttga cgtttttaat gccaagatgg cctcctttta atgaataaga
     7801 cttctaacaa gagagattag tgattcagct ccaaaaccaa ccccggcacc gatgagtgca
     7861 tagaccatgc gctggcgggc ctggggatgt ccgatgacga agcttagccc cgcccaggaa
     7921 agacccacga tggctgcaag aggcaaaatt gttcccacta atttgtcctg caccgccgag
     7981 agggagcttt cgacactcgc ctgggcaaat ggggcggtgg ttaagaccac cgccaaaggc
     8041 agtaaaaatt tattcgccaa agtgcctgtt ctttcttgga tattttttac tttcgtaatc
     8101 acaaaattct tcataggtca tttcctccgg acgggttaat agttttgcca aaaattcttt
     8161 gagttttttt aaaagtttca taaatcatcg tcctcacttt catcttcgaa atagttgtcg
     8221 tcatcaccgc ccccgaacat ttcggactca agggcgttca tgatgttaga aaatgtatct
     8281 tccaactctt tttgctcgat catgggaatt agatcccaaa ggttttctaa gtgattacga
     8341 attgtttccg cgtcgctctc gtcctcgagt aaatttttag cctcttcaat ggcttcgtgg
     8401 agctcgtcgg catcaacacc gggactaatt gattctaaaa actcttcgat ttcctgaacg
     8461 agatattcat tttttaagca catgacggac ctccatcggt ttcacaagta gtggacattt
     8521 cagtaacttt tattttcagt ttactaacgg cggcgtcgca gatttgttca gcgaagttga
     8581 cgccaattcc aagatcacgg gcgatttgct tgaatgagta atcacaccaa aacctcagat
     8641 aaatgatttg ctgttgtcgt tctgaaagct cacgaacggc ctgagaaagt tcgcggtgtt
     8701 tgatctccgc gagttcctct ttgtaatttt tagggtcgta caccttttcc tccctggcat
     8761 aatttgagag aggagcttcg atcgaagaat cttcacctct catataccgg cgggtgatta
     8821 cttcgttccc ttgaattccc cgctggaggc gtagaagtgc tctatttttg atctgatgaa
     8881 ctcctggttg agatcggttc acttcggcag cgatttcccg ttccgtcttt ccgttgagaa
     8941 aaaccaatga aagaactctt ctttccattt ttggaagttc gtttaaaagg tcttcgcaga
     9001 gttgggtttt ttcgggcgag gtgtgataac caaactgaga aaaaatactt tcttccattt
     9061 gttcacattt ctctgaatag agagtggcaa ggacaagagc ctcacgacgg ccgctctcat
     9121 accatttaag atagcgctcc caggttggag ggtcccaatt ttgacagaga ttcataatct
     9181 gctctgttgg gatctcgact ccgccagaag tcagccaagg ttttggtaat tgcttttgca
     9241 ttttcatctc ctgcacgact ttgttggttg atgcgtgcga ggagacctaa atgcgaatgt
     9301 ggcaccgttc agtgccaggc cgctaagcgt ttgaattgct atatgaatat tgagttttgc
     9361 gaggtatttc caaaaatgga aaaaccgcca taatttccat ttttggaaga gggttcggac
     9421 ggaattgagt taattcgagg agttaggagt tttcattttt ggtaattttc aaaagccgtg
     9481 gaaattggct ggagctaggc cgagcggcta ggatattggg aatggaaggg gcttgggggc
     9541 gaggggtcta gcttgtcaga ctaataggct ggcgatcgtg atcggtcgaa gtggggacgg
     9601 ggagagtgtt ccccaaagta aaagacgaca actcaatatg agttgtcgtc cgataggtgc
     9661 ttcaaagcgt agtgtttttg ataacgcaga agagaatttt tcatttgaaa atttttagat
     9721 tcctaaacga tttaaacact tgctaatgaa atttccggtt cgcaattttc tcgaaaaatg
     9781 aaaaataaga agtttttcga atttattgtg cgactttcaa ggcggcatat tttcagtatt
     9841 acattcgtca atcgaacaag tcgattttct cttgttgcga gagtcatgaa tgagaccttt
     9901 gttgattttc gagcttcaaa aggtttactt ttcttgtcgc agtttctctt taaaaaaagt
     9961 ctgcacttca tctatgaagg ctgaatggac tttgcggtga tatggctgat taaagaattg
    10021 cagataaaca gaaactcgca agacacgctt gggttccaga atttctagag tggattcatg
    10081 ctcaataagg tggagaggta caatcgcttt tccatagccg tttttgacgc catcaattag
    10141 tccatagacg tcatcaaggt agcgcatcgt cagcggcttg aatggcaatt tattttgaga
    10201 aaaatagctt cttgtcgtta tatcattttc gtcatggtcc aagtaaatat ctacgtccgg
    10261 cgcccttttt agcttcacct gaacattgtc t
//
LOCUS       DATBTE010000133         3065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_427769, whole genome shotgun sequence.
ACCESSION   DATBTE010000133 DATBTE010000000
VERSION     DATBTE010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427769"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            298..2034
                     /locus_tag="VIG33_04975"
     CDS             298..2034
                     /locus_tag="VIG33_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270218.1"
                     /translation="MHLDAFIKITSEHFLAFNQFLLKNKAPRGIGARLKGPIQNIAKD
                     FDDGLPLLTLPLLDTIPSESREPLEEDAYCQLTEALKSHVDGLYKKLSFRDLVHEADP
                     YVYKELIRDIKDNIFSWEPCSKRVLRTLILNGHPFSVALMEYKDSIEKCKGTTDEIKN
                     IVNIIYMRYIHVKKNNKSSPPLPCFEDVLELYFPTIVDQTAVGLFIMLQTGWNKEVLT
                     ALDGNNFEHVLTGAVNSNQSLVVSEKNKSQNNDKPFKKPKRFIAPSDKSDKYSVYNLI
                     ALARDLSAPFVDLPIDVGIRDATELYNPLFLCMRNWKRWLSVSRSTGENTGRYYSIKH
                     KNLWTNGIERFFELYEVNEKNTRLKTAQDINGRLRPTWITYTRNKKKHPLSLIALQQG
                     HASVETTDVYYDSSGPAMQERRKRLRTELNSILSLLQARQFKGLIGKKGNIPASNDSI
                     RIFTIPSHNKSLWACTNPYQPDWAGHESSIIADKKCNQISMCLFCSRVCIIEDSLPYL
                     MKRQSTLQEELESFPEIEFNSPLTDELEIIQFILQEWNDESALKEASRYLRRNPGLLP
                     RDMQSLSILFED"
     gene            2040..>3065
                     /locus_tag="VIG33_04980"
     CDS             2040..>3065
                     /locus_tag="VIG33_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270219.1"
                     /translation="MMSKESKEDDELLDILEQENKEASRFQFDEILNASTGSIQSVRG
                     LITKLDACPNQDFSVSDYSHFRDDRWELMKKDGFITKRVLFNSELEDSNYLKKLITYH
                     LIPAFNPFGTIRSFESTQTYAFAHSLAETYIFRANGLTATPKDINLITAKMLNNALDS
                     ARDDGAGRHYNFLFFYIRFWLSLSDQDLIPKEYCLDVATRLIDTKERHQDIINEIEAK
                     HQGWKSYTESDLANLIDYALFWTEKAIPVLLEARDYVESKGISLQKHDHYIQPKDDLQ
                     FEAFFGKKIDGIEIIGFTKRIVKSFVDEHSYSYPHYYWKYTYALALDRVRNGLFILIA
                     LITGMRRK"
BASE COUNT          995 a          551 c          614 g          905 t
ORIGIN      
        1 aaagcgaaag aaatctatag cgtcattgtt agcctataaa tctcttaacg ttttatgacg
       61 tacagaacaa ttgtatccca tgtaagggaa gtcgaatctt caaatggttc aatagtcaaa
      121 tgtacttatt tggaccattt gcgtttttcg aatgattgtg atctttgttc tggccttcat
      181 tacgtcttcg tgcgattcgc tcctgatagg acgtactcaa aggtttatga gctaagaacg
      241 gtcataaatt tatttctgga ctatgttcat gactatgaag gaaggaaccc taaggccttg
      301 catctcgatg cttttataaa aataacatct gagcatttct tggctttcaa tcagttttta
      361 ctgaagaata aagctcccag aggaatcgga gcaagactta aggggcccat tcagaacatc
      421 gctaaagatt ttgacgacgg acttccatta ctgacacttc cattattgga cacaataccg
      481 tctgaaagta gagagcctct agaggaagat gcttattgtc agctgacaga agcactgaaa
      541 tctcatgtcg atggcttata caagaaactt agcttcagag acttggtcca cgaagctgat
      601 ccatatgtct acaaagaatt gataagagac ataaaagata atattttttc ctgggaacct
      661 tgcagtaaaa gagtactaag aacactaata ttgaatggac accccttctc tgtagcttta
      721 atggagtaca aagatagtat tgaaaaatgt aaaggaacaa cggacgagat caaaaatata
      781 gtaaatatta tttacatgcg ttacatacac gttaaaaaga ataataaaag tagtcctcct
      841 ttaccctgtt tcgaagacgt tttagaactt tattttccga caattgttga tcagactgct
      901 gtagggcttt ttattatgct ccagacaggg tggaataaag aagtattaac ggcattggat
      961 gggaacaatt ttgaacatgt tttgaccgga gctgttaatt ccaatcaaag tctggttgtt
     1021 tcagaaaaaa acaaatctca aaacaacgat aagcccttta agaagcctaa gaggtttatt
     1081 gctccaagtg acaaaagtga caaatattca gtttacaacc ttattgcctt agcgcgtgat
     1141 ctttctgcac cgtttgttga tctgccaatt gatgtcggta tcagagatgc tacggagctt
     1201 tataatccgt tgtttttatg catgcgtaat tggaaaagat ggttgtcagt aagtcgaagt
     1261 acgggggaaa atacggggcg ttactactct ataaagcaca aaaacttgtg gactaatgga
     1321 atagaaaggt tttttgaact gtatgaagtt aatgagaaaa acactcgcct aaaaactgct
     1381 caagacataa atggtcgact acgccccaca tggataactt ataccagaaa taagaagaaa
     1441 cacccgctta gtcttatcgc acttcaacag gggcatgcca gtgttgaaac tacggatgtc
     1501 tactacgata gctctggccc tgcgatgcaa gagaggagaa aaagattaag gactgaactt
     1561 aattcgatat tatcattatt acaggcaagg caatttaagg ggctcatagg caaaaaggga
     1621 aatattcctg ctagtaatga ttcgatacgt atctttacta tacctagtca taacaaatca
     1681 ctttgggcat gcacaaaccc atatcaaccg gattgggctg gtcatgaatc aagtatcatc
     1741 gcagacaaga agtgcaatca aatttccatg tgcttgtttt gcagtcgagt ctgcattatc
     1801 gaagactcct tgccctattt gatgaaacgc caatctacac tccaagaaga gttggagtct
     1861 tttcctgaaa tagagtttaa ctcaccattg actgacgagc ttgagattat ccagtttatt
     1921 ttacaagaat ggaatgacga atcagcctta aaagaagcgt cacgctactt gcggcgcaat
     1981 cctggattat tgccgcgtga tatgcaatct ctttcaattt tatttgagga ttaattacca
     2041 tgatgagcaa agaatccaaa gaagatgatg agttattaga tattcttgag caagaaaata
     2101 aagaagcttc taggtttcag tttgatgaaa tattaaatgc ttctactggc agcatccaaa
     2161 gcgttagagg acttattaca aagttagacg catgccctaa tcaggatttc tccgtctcag
     2221 attactctca ttttagggat gatcgatggg agcttatgaa gaaggatggc ttcattacga
     2281 agagagtgct ttttaatagt gaacttgaag acagtaacta tttaaagaaa cttatcactt
     2341 atcatttaat accggctttt aacccgtttg gtactataag aagctttgaa tctacccaga
     2401 catacgcatt cgcacactca cttgcagaga cttacatatt tagagctaat ggtcttaccg
     2461 ctacgccaaa agatatcaac ttaattacag ccaaaatgct caacaacgca ttagactctg
     2521 caagggatga tggagctggt aggcattata attttctttt tttctacatt cgtttctggt
     2581 tgtctttgtc tgaccaagat ttgataccca aggaatattg cttagatgtg gcaacccgat
     2641 taatagacac taaagagcgt caccaagata tcatcaatga aatcgaagct aagcatcagg
     2701 gctggaaatc ttatactgaa tctgatttgg ccaacttaat agattacgca ttgttttgga
     2761 cagagaaggc aattccagtg cttttagagg cgcgagacta tgtggaatca aagggtattt
     2821 ctttgcaaaa acatgatcat tatattcaac caaaagatga tttgcagttt gaagcgttct
     2881 ttggaaagaa aattgatggg attgaaatca ttgggttcac aaaaagaatt gtaaaatctt
     2941 ttgttgatga acattcatat agttatcctc attattactg gaaatataca tatgcgcttg
     3001 cccttgatag ggtgagaaat ggtctgttta tcttaatcgc tcttatcaca gggatgcgaa
     3061 gaaaa
//
LOCUS       DATBTE010000134         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2272055, whole genome shotgun sequence.
ACCESSION   DATBTE010000134 DATBTE010000000
VERSION     DATBTE010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2272055"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VIG33_04985"
     CDS             <1..417
                     /locus_tag="VIG33_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8270220.1"
                     /translation="IHKQYEPIPLVSCYASQINQVLMNILSNALQAIEGPGHIWISTI
                     ALRNTADKNGKVQVSIQDSGKGMKADVLEKIFDPFFTTKGIGQGTGLGLSISYGILQS
                     HGGEIQVRSEVGVGTEFTIIIPTYPPANRLTKKDEI"
     gene            617..1099
                     /locus_tag="VIG33_04990"
     CDS             617..1099
                     /locus_tag="VIG33_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270221.1"
                     /translation="MDSPASSVDPKEFESKVNNHLFNTAQSIEMSQEKMRMETDQLAD
                     QGLNPQASPKVEVQRLDFSMDPRSETLLNELGREVKESTGPSNADETIQADLFESQQS
                     QELSEAYKKEYARQFIENARKAGYIIRLNEDYKAISVRPIRKPAEDMELFPSNGHGTQ
                     "
     gene            1210..1821
                     /gene="lpoB"
                     /locus_tag="VIG33_04995"
     CDS             1210..1821
                     /gene="lpoB"
                     /locus_tag="VIG33_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HEY8270222.1"
                     /translation="MNRLVRSMTTLAVATVSALVLSSCGPKAFVKGDYEDPEKANLMN
                     DQWSETDMQTVVRDLVGSLTFHPAIKDAKNPPVVMVTQLQNKTSEHIDTQSVMDMVRV
                     DLMKTGKVGFIDKEARQDIADEYNYQNSGMVAKDSKKGPGGQTGADFIINGRLDSIVQ
                     EVGKDKTVYYKVTLNMTNLKTGMIVWSDNKQLRKAYKKKTIGL"
BASE COUNT          617 a          357 c          406 g          467 t
ORIGIN      
        1 atccataaac aatatgagcc gatccccttg gtctcttgtt atgcaagcca gatcaatcaa
       61 gtgctgatga atattctttc taacgcttta caagcgatcg agggacctgg acatatttgg
      121 atttccacca tagcccttcg caataccgca gataaaaacg gcaaggtgca agtttcaatt
      181 caagacagcg gaaaaggaat gaaggcggat gtccttgaaa aaatcttcga tccttttttc
      241 accacgaaag gcataggcca agggacaggg ttaggattaa gtatttctta tggcatattg
      301 caaagccacg gcggagaaat tcaagttcgc tcggaagtgg gtgtcggcac ggaattcaca
      361 attattattc cgacctatcc gcctgcgaat cgtttaacta aaaaagacga aatctaaagt
      421 gttccaggta ctaagtccgg gtgattaaag cgctctctgg gattttccga taaagtgaag
      481 gatatataat agagggttta tgggaaaatc caagaaagag aaaaaagagg atagatctta
      541 cctcctcgga atagctttgc tattgctatc aggttttttc atatttcttt ttttcctgaa
      601 cgagccagag aaaagaatgg attctccagc cagttcagta gatcccaaag agtttgagtc
      661 caaagtaaac aatcatcttt ttaataccgc tcaatccata gaaatgtcgc aagaaaagat
      721 gcgaatggaa actgatcaac ttgccgatca aggtcttaat ccgcaggcct cccccaaagt
      781 ggaagttcaa cgtttggatt tttcaatgga tcccagatcc gaaacactcc tgaacgagct
      841 agggcgggaa gtcaaagagt ccaccggacc aagcaatgcc gatgaaacga ttcaagctga
      901 tctcttcgag tcacagcaaa gtcaggaact ctctgaagcc tataagaagg aatacgcccg
      961 ccaatttatt gaaaatgctc gaaaggctgg atatattata agactgaatg aagattacaa
     1021 agcaatatct gtcagaccta ttaggaaacc ggccgaagac atggaacttt tcccaagtaa
     1081 tggccatgga actcaatgaa gcgtagtctc aggccttgta tattacagag aagtttctaa
     1141 aatactaaag tgcatattcg gtgcaactgg aataaaacaa aagtaattta aaaatcgtgt
     1201 ggaggtatta tgaatcgcct ggtgagatct atgacaactt tagcagttgc tactgtatca
     1261 gcacttgtgc tttcaagttg tggaccaaaa gctttcgtca aaggagatta cgaagatccc
     1321 gaaaaggcca accttatgaa cgaccagtgg tcagagactg atatgcaaac agtggtgagg
     1381 gacttggtcg gcagcttgac gtttcatcca gccattaaag atgcgaaaaa tccccccgtg
     1441 gtgatggtca cccaactgca aaataaaacc agtgaacaca ttgataccca gagtgtgatg
     1501 gatatggttc gtgtggatct tatgaaaact ggtaaagtag gtttcatcga taaagaagcc
     1561 cgccaagata ttgcagacga gtacaattat caaaactctg gtatggttgc aaaagactct
     1621 aaaaaaggtc cgggcggtca aacgggcgct gactttatta tcaatggacg tttagattcg
     1681 atcgttcaag aagttggaaa agataaaaca gtttattata aagtaacgtt gaacatgacc
     1741 aaccttaaaa caggtatgat agtgtggtca gacaataaac aactgcgtaa agcttataaa
     1801 aagaaaacta tcggtttgta aaatatgaat ttagttctta aaagatc
//
LOCUS       DATBTE010000135         8848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1042739, whole genome shotgun sequence.
ACCESSION   DATBTE010000135 DATBTE010000000
VERSION     DATBTE010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 8848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8848
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042739"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VIG33_05000"
     CDS             <1..685
                     /locus_tag="VIG33_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEY8270223.1"
                     /translation="KYYNKASGEEVSHNDAVKAFEYKPESYVIMTEADFKKANPKATQ
                     TIDIESFVGLEEIDPVFYEKAYYLLPNKGGEKAYSLLVEALQQTRKVAIAKIVLHTKQ
                     HLAALIPRGKFLLLELLHFAEDVKELRELGDWKAEVPVSRTLHREVEMAEKLIEDMTT
                     KWEPSDYEDTYRADIMKQVKAKVKAGKATEITRDFEEVKEEKAPQLVDLMPLLRKSLA
                     AKKTKSTHH"
     gene            707..3073
                     /gene="ligD"
                     /locus_tag="VIG33_05005"
     CDS             707..3073
                     /gene="ligD"
                     /locus_tag="VIG33_05005"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEY8270224.1"
                     /translation="MSLREYRKKRDFSKTKEPLTGKRTAKPPIFKAPIFVVQEHHTRH
                     LHYDFRLEAFGTLKSWAVPKGPPMEVGEKRLAVEVEDHPLEYAKFKGRIPEGQYGAGL
                     VKIWDRGTWIPPQHLRKELKNGHLEFELKGKRLRGRWLLQRTKNRSGRKNQWLLIKRH
                     DKAKLTDKLRGEPAPLPIQLAPELATLVAAVPRGDEWLHEIKFDGYRTLAILNKGKVE
                     LQSRRGLDWTEKYRPLDKEIKKLKVQSGIFDGEIVVLDEDGRSSFSSLQEALKVLNKD
                     VKGNELIYYLFDILYVNGRNLCEEPLEVRKMILKKILLSSSVKNIRYSEHIRGQGEEI
                     FVQSCKSGFEGIVSKNRTKSYRMKRTTDWLKLKCSHRQEFVIGGYTDPTGTRESFGAL
                     LLGVYESDQRKDKLRYVGRVGTGFDSKMLSDLFPQLQKLRIATSPFEVASPKKTKNTH
                     WVQPSLVAEIEFKAWTHQNILRQASFIALRADKSPKEITKETPLVLKKKAQSARKGSF
                     RITHPERVLYPASQVTKLDVARYYKSAAPWILEYVSKRPMSLLRCPTDVTSGCFFQKH
                     VTHSNLTAVDEGVVEDQSFLFINSEEGLLQLVQSGVLEFHTWQCSVTDPEKPNQVVFD
                     LDPDEDVPWREVVKTAFRFKQLLSKISLKSFVKLTGGKGLHVHIPIAPLYSWDEVKAF
                     AKAIAYQLKSEHPDFYTLNMAKKKRSGKIFLDYLRNGLGATAIAPYSLRAREEPFVAL
                     PLSWPELKNMKRLKKFDMNIALMHLASARANPWPRYNQLEQRVRLLEV"
     gene            complement(3075..4925)
                     /gene="ftsH"
                     /locus_tag="VIG33_05010"
     CDS             complement(3075..4925)
                     /gene="ftsH"
                     /locus_tag="VIG33_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEY8270225.1"
                     /translation="MEAKRNYSWIFFFISMALVFSLQNIWLSSRQLAEIPYSEFQKLL
                     NDKKVDNLTISDQFIRGEFKEPRPDKKKEFFTARLPTDLAKDFAGSGVTYTRVVESRF
                     FRDLLSWTIPALIFVGLWFFLSRVIAGKGGFGAGLMSIGKSRAKLYVETDVKVTFADV
                     AGVDEAIDELQEVVEFLKDPTQYSRLGARMPKGILLIGPPGTGKTLLAKAVAGEAKVP
                     FFSISGSEFVELFVGVGAARVRDLFVQAREKAPCIIFIDELDALGKMRSTFMGGAGGH
                     DEKEQTLNQLLAELDGFDTKSGIILLAATNRPEILDPALLRAGRFDRQVLVDRPDKKG
                     RESILRVHLQKIKYDPHLQVDYLAALTSGFTGADLANLINEAALIATRRKAAIVDQSD
                     FFNAIERTVAGLEKKNRILNPKERRTVAFHEMGHALVAGAIPGCDKPQKVSIIPHGIG
                     ALGYTIQRPTEDRYIITRDELLSKMAVLLGGRAAEMLVFNELSTGAANDLVQVTNIAE
                     ALVSQYGMSNALGTVVFDRHMSPFLQGPFPDYEPRHYSEETANLIDREIKNVIEEALL
                     KAKIILQQNRHVLEAGAQLLLEKETLSENELKTLFLQIVGAAPEILSKDS"
     gene            5293..6459
                     /locus_tag="VIG33_05015"
     CDS             5293..6459
                     /locus_tag="VIG33_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8270226.1"
                     /translation="MEWKELWSELHRWVELNKPLAGEGIKEIELKGVSMGNDIIFYQL
                     FEAESSTYTYVIADKKTKEAAIIDPVLETVDRDLKLINELDLRLMYILDTHIHADHIT
                     GAGEIRNHTKAKTAVSHKAEVSCVDIPLEDGQELLLGDKKIKVIATPGHTNTCITFAF
                     EGMIFTGDALLIRGCGRTDFQQGSADKLYESVHEKLFKLPEDTLVYPGHDYRGQTSST
                     IGLEKKFNPRLGEAKSKEEFKNIMSELKLANPKKIHEAVPANLACGKPKESRTIYPQV
                     VDGIPEINCEDVFAHLPQVESKKIRLIDVRRPDEFNNEYGHIHGAELVTLGAELMGFL
                     EKGNRSEEIIFICRSGGRSGTAAAESIKLGYKYTINMVGGMIRWNEKKFPVERN"
     gene            6482..6907
                     /locus_tag="VIG33_05020"
     CDS             6482..6907
                     /locus_tag="VIG33_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEY8270227.1"
                     /translation="MHQDWIQAIIGGAIIGISVSLMLLLNGRVTGISGIINGVLSHVK
                     GDTAWRILFIAGLFLGGLVLRFLKPDAFSGALPAENWTVVGAGLLVGFGTILGSGCTS
                     GHGVCGISRMSLRSLTATVVFILAGIVSVAIFRKLGVFP"
     gene            6904..7332
                     /locus_tag="VIG33_05025"
     CDS             6904..7332
                     /locus_tag="VIG33_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEY8270228.1"
                     /translation="MKNAFVALGVGFIFSLGLGISGMTQPQKVVGFLDVFGSWDPSLM
                     FVMIGAIAVHFITYKLIRKRKSPLFSKQWHVPTKKEITPALIVGSFIFGIGWGLGGFC
                     PGPAVTSLASFEMRPFIFVLSMLVGMIIFRQLDKKIKIEK"
     gene            7346..8158
                     /locus_tag="VIG33_05030"
     CDS             7346..8158
                     /locus_tag="VIG33_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8270229.1"
                     /translation="MEIYGYFASILMGLSLGMIGGGGSILTVPILVYLFSINPLIATS
                     NSLFIVGSTALVGGVLALRRKEVSVRSGMLFALPSFVGVFLAKSILLPKIPEHIFSFQ
                     GMILTKALVIMGIFSILMLLASLAMILKKNMPQSADTEEASGEKNKWNSILVQGFLVG
                     CMTGLVGAGGGFLIVPALVNLVGLSMRMAIGTSLMIIAANSLFGFSVALAKGLSVDWI
                     FLGSILCLALIGLVLGSFFSKRVSEKKLKTGFGYFVLVMGTLILLDQITKLS"
     gene            8482..>8848
                     /locus_tag="VIG33_05035"
     CDS             8482..>8848
                     /locus_tag="VIG33_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270230.1"
                     /translation="MISNPQAQASTASSGITYTGRILDASDAPVIDSSVIFTVTIYDP
                     AGKCWLYTEQRKLDLSQTAGTFSFEIGSNDSATLYGAPPSFNNQSSGGPKNLADLFSN
                     KKSYLGLGTANGCTGTYDPS"
BASE COUNT         2441 a         1802 c         2161 g         2444 t
ORIGIN      
        1 taagtattat aacaaagcct cgggcgaaga agtttctcac aatgacgccg tgaaagcatt
       61 tgagtacaaa cctgaaagct atgtcattat gacagaggcg gattttaaaa aagccaatcc
      121 aaaggcgaca cagaccattg atatcgagag ttttgtggga cttgaggaaa ttgaccccgt
      181 attttatgaa aaagcttatt atctcctgcc taacaaaggg ggtgagaagg cttactcatt
      241 attggtggag gctcttcaac aaactagaaa agtagcgatc gcgaaaatag tgctgcacac
      301 gaagcagcat ctagcagcac tgattccccg gggaaaattt cttcttcttg aacttttgca
      361 ttttgcggaa gacgttaagg agttgcgtga actgggggat tggaaagccg aagttcccgt
      421 ttcacgaact cttcacagag aagtggagat ggcagaaaag ttgattgagg atatgactac
      481 caagtgggaa cccagcgatt atgaagatac ctatcgcgcg gatatcatga agcaagtaaa
      541 agcgaaagtg aaggcgggca aagccaccga gattacgcgt gatttcgaag aagtcaaaga
      601 agaaaaagca cctcagctcg tggatttaat gccccttctc cggaagagtt tagctgcaaa
      661 aaaaacgaag tccacccatc attaggaacc ctctaagagg tcttttatga gtttgagaga
      721 atatcggaaa aaaagggatt tctcgaaaac caaagaacct cttacaggaa agagaactgc
      781 gaaaccccct atttttaaag cccccatttt tgtagtgcag gaacatcata caaggcactt
      841 acattacgat ttccgcttag aagccttcgg gactcttaag agttgggcgg tcccgaaggg
      901 gccgcctatg gaggtgggtg aaaaacgttt ggcggtggag gtggaagatc atccccttga
      961 gtatgcaaaa ttcaagggcc gtattcctga aggtcaatac ggagccgggc tcgtgaaaat
     1021 ttgggaccgt gggacgtgga ttccacctca acatcttcgg aaagaactaa aaaacggaca
     1081 cttggaattt gaactcaagg ggaaacggct tagaggacgc tggctgcttc agcggacaaa
     1141 aaataggagt ggtcggaaaa accaatggtt attgatcaaa cgtcatgaca aagccaaact
     1201 tacagacaag ctcaggggcg aaccagcccc cttgccgatc cagcttgctc ccgagttggc
     1261 caccttggtg gcggcggttc ctcggggaga tgagtggctt catgaaataa aatttgatgg
     1321 gtatagaacc ttggccattt taaataaggg caaagtggaa ttgcaaagtc gccgcggtct
     1381 cgactggact gagaaatacc ggcctctgga taaagaaatt aaaaagttga aggttcaaag
     1441 tggaattttt gatggggaaa ttgtggtcct tgatgaagat ggaagatcca gcttttcctc
     1501 cttacaggag gctttgaaag tcttaaataa agatgtcaaa ggcaatgaac tcatctatta
     1561 tctgtttgat attctttatg tgaatggcag aaacctttgt gaggaacctc tagaagttcg
     1621 gaagatgatt ttaaagaaaa tacttctttc ctcaagcgtg aaaaacatcc gttacagtga
     1681 acatatacgt ggccaaggag aagagatctt cgtgcagagt tgcaagagtg gttttgaggg
     1741 aattgtttca aaaaatcgga ccaaatctta tcgcatgaag agaaccacgg attggcttaa
     1801 gttgaaatgc tctcatcgcc aagaatttgt tattggcgga tatacggatc ccacaggaac
     1861 cagagaaagt tttggtgcac ttcttctagg cgtttatgaa tcggatcaga gaaaggacaa
     1921 attgaggtat gtaggtcgag tgggaaccgg ttttgattcc aaaatgcttt ccgatctttt
     1981 tccccaattg caaaaactac gaattgcaac cagtcctttc gaggtggctt ctccaaaaaa
     2041 gacaaaaaat actcactggg ttcagccaag cttggtcgca gaaatcgaat tcaaagcctg
     2101 gacccatcaa aacattttgc ggcaagcgtc ctttatcgct ttgcgagctg ataaatctcc
     2161 aaaagaaatc accaaggaaa ctccgctcgt cttaaagaaa aaggcgcagt ctgcgcgcaa
     2221 ggggtctttt agaattacgc atcctgagcg ggtgctttat ccggcttcgc aagtgacgaa
     2281 actggatgtc gcccgctatt ataaaagtgc agcaccttgg attttggaat atgtctcaaa
     2341 gcggccgatg tctttgttgc gttgtccaac cgatgtgacc tcaggttgct tttttcagaa
     2401 acatgtaact cattccaatc ttacagccgt tgatgaaggc gtggttgaag accaaagctt
     2461 tctctttatc aactcagagg aaggactatt acagctcgtt caatccggag tcttggaatt
     2521 tcatacctgg cagtgttccg tgacagatcc agaaaagccc aatcaggtgg tttttgatct
     2581 tgacccagat gaagatgttc cttggaggga agtggtcaag accgcctttc gttttaaaca
     2641 gcttttatca aaaatatcct tgaagtcatt tgttaaattg accggaggaa aagggctgca
     2701 cgtccatatt cctatcgcgc ccctctactc ctgggatgaa gtgaaagctt tcgccaaggc
     2761 tattgcttac cagttgaaga gtgaacaccc cgatttttat actttgaata tggcgaagaa
     2821 aaaaagaagc ggaaagattt ttcttgatta tttaagaaac gggttggggg ctacggcgat
     2881 tgccccctat tctttaaggg ctcgcgagga gccctttgtg gccctgccac tgagttggcc
     2941 ggaacttaaa aacatgaagc ggctgaagaa attcgatatg aatatagctc tgatgcatct
     3001 tgcctctgca agagcaaatc cttggccaag atataaccaa cttgagcaaa gagttcgttt
     3061 gttggaagtc taatttaaga gtctttagag agaatctcgg gggcagcacc gacgatttgg
     3121 aggaataaag ttttaagttc attttctgaa agggtttctt tttcaagaag gagttgcgcc
     3181 ccggcttcca gcacatgacg gttttgttgc agaatgattt ttgcttttag cagagcttcc
     3241 tcaataacat tttttatttc tcgatcaatc aggttagcag tttcttcgct gtaatggcga
     3301 ggttcataat ctggaaaggg gccttgcaaa aagggactca tatgccggtc gaagaccacg
     3361 gtaccaaggg cattactcat gccatattgg ctgaccaatg cttctgcaat gttggtgacc
     3421 tgcactaaat cattggcggc tcccgtggag agttcgttaa agaccagcat ctccgcagcc
     3481 cgtcctccta acagaacagc cattttcgaa agaagttcgt cgcgagtgat aatatagcgg
     3541 tcttcagtgg ggcgttgaat ggtgtaacct agggcgccaa ttccgtgcgg aatgatcgag
     3601 actttctgcg gcttatcaca acccggaatc gcaccagcca ccaaagcatg gcccatttcg
     3661 tgaaaagcca cggttcttct ttccttggga ttaagaatgc ggtttttctt ttccaggccg
     3721 gccacggtgc gctcaatggc attgaaaaaa tcgctttggt ccacaatcgc ggctttgcgg
     3781 cgggtggcga tgagtgccgc ttcgttgata agatttgcca ggtcggctcc cgtaaaaccc
     3841 gaggtgaggg ccgcaagata atccacttgc aaatgaggat cgtatttaat tttctgtaga
     3901 tgaactctta aaatggattc gcggcccttt ttgtcgggac gatccacgag gacttggcga
     3961 tcaaagcgtc cagctcttaa tagcgcggga tctaaaattt caggacggtt ggtagctgcg
     4021 agcaggatga tgcccgactt ggtatcaaag ccatcaagct ccgctaaaag ttgattcaga
     4081 gtctgttctt tttcgtcatg gcctccggct cctcccatga atgtggagcg cattttaccg
     4141 agcgcatcga gttcatcgat aaatatgata cacggggcct tttcccgagc ctgcacgaaa
     4201 agatcccgca ctcgggcggc accgacgcca acaaagagtt ctacgaactc agatccgcta
     4261 atagaaaaaa aaggaacttt ggcttcacca gccacggcct tggccaaaag ggttttgccc
     4321 gttccaggag ggccgataag aagaattccc ttgggcatac gtgcgcccaa tcggctgtat
     4381 tgggtagggt cttttaaaaa ctccaccact tcttgcagct catcgatggc ttcatcgact
     4441 ccggcgacat ccgcaaaagt cactttcaca tccgtttcaa cgtagagctt cgcgcggctt
     4501 tttccaatgg acataagccc ggcaccgaag ccacctttgc cagcgatcac gcggcttaaa
     4561 aaaaaccaca ggccgacaaa gatcaaagcg ggaatagtcc aggaaagaag atcacggaaa
     4621 aacctgcttt caacaacgcg ggtgtaagtc accccacttc cggcaaagtc tttagcgaga
     4681 tctgtcggaa gacgcgcggt gaaaaattct ttttttttgt cggggcgggg ttctttaaat
     4741 tctccgcgga tgaattgatc gctaatggtt aggttgtcga cttttttgtc attcaaaagt
     4801 ttttgaaatt cactgtaggg aatttccgcc agttgccttg aactcaacca tatattttgc
     4861 agcgagaaga cgagggccat ggaaataaag aaaaagatcc aagagtagtt tctctttgct
     4921 tccatttagc ctccgcttat gacgcgtatt atgtaaatta aaaatataag gggtatcaaa
     4981 gcactaaaaa agcatttcgg cgagtgacga aattgcgaaa aatcccagaa ccctttagag
     5041 gttttaagtc tttggaagtc agcaacaaca aagagactat taagtgtgca gcaaattgaa
     5101 tttcgatttg ggacgtctac caaaagacct cgcctcttca aatcctcgga gaggcgaggc
     5161 ctcaattctt caaataagat atgattcatt ccgtatcatc gctccgcctc cagcacatca
     5221 tttaaatatt ttttgaacca gcacgtcaca taactagttc ccaatcaggt ttagattgaa
     5281 tatgatacgc ccatggagtg gaaagagctt tggtctgagc tgcatcgttg ggtggagttg
     5341 aacaagcccc tggctggcga aggaattaaa gaaatagaac taaaaggagt atctatggga
     5401 aacgacatta ttttttatca actatttgag gcggaatctt caacttacac ttatgttatt
     5461 gctgataaaa aaaccaaaga ggcagctatc attgatcctg tcctggagac tgtggaccgt
     5521 gatcttaagc ttatcaacga attagactta agacttatgt atatattaga tactcatatt
     5581 catgcggatc acattactgg cgccggggaa attcgtaatc acaccaaagc gaaaacggcg
     5641 gttagccata aagccgaagt gtcgtgcgtg gatatccctt tagaagacgg ccaagaactt
     5701 ttactgggcg acaagaaaat caaagtcata gctacgccag gccataccaa tacttgtata
     5761 accttcgcct ttgaaggcat gatttttaca ggggatgctc ttttgattcg cggttgtggc
     5821 agaactgatt tccaacaggg ctcggcggat aagctttatg aaagcgttca tgaaaaactt
     5881 ttcaaactcc cagaggatac tttggtttat cctggtcatg attatagagg ccaaacctct
     5941 tcgactattg ggctggagaa gaagtttaac cctcgcttag gcgaagctaa atctaaagaa
     6001 gaatttaaaa acatcatgtc ggaactgaag ctggcgaacc ctaaaaagat tcacgaagcg
     6061 gttcctgcga atttggcttg cggaaaacca aaggaatcac gaactattta ccctcaagtg
     6121 gtggacggga ttccagaaat caactgcgaa gatgtatttg ctcacttacc tcaagtggaa
     6181 tcgaagaaaa ttcgtttaat agatgtaaga cgtcccgacg agttcaataa tgaatatgga
     6241 catattcatg gagctgagct cgttactctt ggcgctgagt tgatgggctt tttggaaaaa
     6301 gggaatcgca gtgaggagat catctttatc tgcagaagcg gtggacgttc aggcaccgct
     6361 gcggctgaaa gtattaaact gggttataaa tacaccatca atatggtcgg cggcatgatt
     6421 cgctggaatg aaaaaaaatt tccagttgaa agaaactaaa agttaaaggc aaggggcggt
     6481 tatgcatcaa gattggatcc aggcaattat aggcggtgcg atcattggaa tttcggtttc
     6541 attgatgttg ctgcttaacg gaagagtgac ggggattagc ggaattatca atggagttct
     6601 ttctcacgtt aaaggtgata ctgcttggag aattctcttc attgcggggc tctttttagg
     6661 aggcttggtc ttgagatttt tgaagccaga tgctttttcc ggagctttgc ccgctgaaaa
     6721 ttggaccgtg gtgggagctg gtttgttggt gggatttggc acgatcctag gcagcggttg
     6781 caccagcggt catggagtct gtgggatcag ccggatgtcg ctgcgctcgc tgactgccac
     6841 ggtggtattt attttagccg gaattgtttc ggtggcaatc tttcgcaaac tgggggtttt
     6901 tccatgaaaa atgcattcgt cgccttaggt gttggattta ttttttcctt ggggcttggc
     6961 atctcgggaa tgactcagcc tcagaaggtg gtgggatttt tagatgtttt cggttcttgg
     7021 gatccctctt taatgtttgt gatgatcggt gcgatcgccg tgcatttcat aacctacaaa
     7081 cttattcgga aaagaaagtc tcctcttttt tcgaagcagt ggcatgtgcc gactaaaaaa
     7141 gagatcactc cagcgttgat tgtgggttct tttattttcg ggattggatg gggattgggg
     7201 ggattttgcc cagggccggc agtgacctca ctggcaagtt ttgaaatgag gccctttatt
     7261 tttgtgctga gcatgcttgt cgggatgatc attttcagac agcttgataa aaaaataaaa
     7321 attgagaaat aagaaatgag tgtttgtgga aatatatggc tattttgcct cgatattgat
     7381 gggactgagt ttgggaatga taggtggagg agggtcgatt ctcacggtgc cgattttggt
     7441 ctatttattt tccattaatc ccttgatcgc cacttcaaat tctttattca tcgtgggctc
     7501 cacggcttta gttggggggg tattggcctt gagaagaaag gaagtcagcg tcaggagcgg
     7561 gatgttattc gcgctcccaa gttttgtcgg ggtgtttctg gctaaatcta ttcttcttcc
     7621 gaaaattcca gagcatattt tttcatttca aggaatgatt ctgactaagg cgctggtcat
     7681 catgggtatt ttttcaatac tgatgttgct ggcaagtttg gcaatgattt taaaaaaaaa
     7741 catgccccag tccgcagaca cggaggaagc ttctggtgaa aaaaacaaat ggaactccat
     7801 tcttgtgcaa ggatttttag taggatgtat gacaggttta gtgggggccg gaggaggctt
     7861 cttgattgtg ccagctttgg tgaatttagt cggtcttagt atgagaatgg ccatcggaac
     7921 ttctttgatg atcattgcag ccaattcgct ctttggtttc agcgtggccc ttgccaaggg
     7981 actttctgtc gactggattt tcttgggttc tatcctttgc ttggctttaa tcggattggt
     8041 gttggggtct ttcttctcta aaagggtttc cgaaaagaaa ctcaaaacgg gttttggcta
     8101 ttttgttctg gtgatgggaa ctttaatttt attagatcag ataaccaaac tttcataagt
     8161 ctttgttagg cctacgggag cgaactattt tcttattttg agacttgtgg tcacgtcggc
     8221 gaattccgcg taaatgcctg tcctttgata taagaactga atttaggagc ataaattact
     8281 tcgaagtgtt tcaccttgag acattagcct caaattttag tttaggaaat tcaaaattta
     8341 gccgattttt aacacatgaa attattttat tgtttgtttt taacgacagc acattgggta
     8401 ttagctgcct ttgggacaaa gtgttttaaa ctaataggcg tgtctctgcg tctttttagg
     8461 tttgtgtttg tgggagtctt tttgataagc aatccgcaag ctcaagcatc aaccgccagc
     8521 tcgggaatta cttataccgg gagaatctta gatgcctcgg acgctcccgt aattgatagc
     8581 tcggttattt ttacggtcac gatatatgac cccgccggga aatgctggct ttataccgag
     8641 caaagaaaat tggatttatc ccaaaccgcc gggacctttt cctttgaaat tggctctaac
     8701 gattcagcaa ctctttatgg agctcctcct tcatttaaca accaatccag tggcggtcca
     8761 aaaaatttag cggatctctt tagcaataaa aaatcctatc tagggcttgg cacagccaat
     8821 ggctgcactg gcacttatga tcccagca
//
LOCUS       DATBTE010000136         2373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2341020, whole genome shotgun sequence.
ACCESSION   DATBTE010000136 DATBTE010000000
VERSION     DATBTE010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2341020"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VIG33_05040"
     CDS             <1..578
                     /locus_tag="VIG33_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8270231.1"
                     /translation="KNEVHLILLDVSLPDGSGFDFCTTLQSQDTTQKIPVIFLTGKIG
                     VGDKVLGLALGAEDYVTKPFDVLELRARVESKLKKFLYYAKEETYFQRGSLKFELSTY
                     SIYLNEEKGNQKLNMTPIEFKLLLKLARSPGYVLSREQLIDSVWGDSVYIEDRSVDRH
                     ISSIRKKLGTCSSYIRTVPGLGYQFLAEQQH"
     gene            597..950
                     /locus_tag="VIG33_05045"
     CDS             597..950
                     /locus_tag="VIG33_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY8270232.1"
                     /translation="MELQFDAVQSIKSIESNYGPQVAREVVQIFINDYPEKLTKLKAA
                     VDQGDIATIRFIAHDIKSGSLSMGIKPMSNICEILERDSSKMNKEELKELSERLAADY
                     FQISKSYGDYLNIKR"
     gene            complement(996..1403)
                     /locus_tag="VIG33_05050"
     CDS             complement(996..1403)
                     /locus_tag="VIG33_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270233.1"
                     /translation="MRTELVFKDVQRSAYAEDFILKKTELLAGKLLQPDKDLHIAVRI
                     EKDRQETANRHPIYQCEVTLKSGISAKTYKTLRQDRNLFRAINSSFEALKLILGKTHD
                     RLRHDRRRRRVPEFVSELPEAETESIPARPLIT"
     gene            complement(1407..2213)
                     /locus_tag="VIG33_05055"
     CDS             complement(1407..2213)
                     /locus_tag="VIG33_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8270234.1"
                     /translation="MNNTDANQHGNNQLVSGYRDYGYRTSEKAPDSHVQAFIPNVLVV
                     EDSVEYQAMIRSSLERDLHLFFASTLKQAKEFLESREVHLILLDVMLPDGSGFDYCND
                     LQTNPATARIPVIFLTGKTGVTDKILGLSLGADDYITKPFVPLELKARIESKLKKSLL
                     HDRNELQLQRGHLRFDTLTYSVYAIKEDKHELINMTPIEYRILLHLAQKPGRIFTRNQ
                     LIDSIWGGTVHINERSIDKHIYTVRKKLGQHGDYIKTIPGLGYQFNIDME"
BASE COUNT          656 a          478 c          541 g          698 t
ORIGIN      
        1 ctaaaaatga agttcatctc attttattag atgtaagtct tccggatgga agtggttttg
       61 atttttgcac aactctgcag agtcaagata cgacccaaaa aataccggtg atttttctca
      121 cgggaaaaat tggcgtcggt gataaagttt tgggattggc tcttggcgct gaagactatg
      181 tcacaaaacc atttgatgtc ttggagctga gggctcgggt agagtctaaa cttaaaaaat
      241 tcctttatta tgcgaaggag gagacttatt tccaacgcgg tagcttgaaa tttgaactat
      301 ccacttattc catttactta aatgaagaga agggaaatca aaaactgaat atgactccca
      361 tcgaatttaa acttttgctt aagttggcgc gctcccctgg atatgtgttg agccgggaac
      421 agctgatcga cagtgtttgg ggggactctg tttatataga ggatcgctcc gtggataggc
      481 atatctcttc cattcgtaag aagttaggga cttgttcatc ctatattcgc acggtacccg
      541 gtttgggcta tcagttttta gccgaacagc agcactaaat gatatgaggg ggacttatgg
      601 aactacaatt tgatgcggtt caaagtatta aatcgattga aagcaattac gggcctcagg
      661 tcgcccgtga ggtcgtgcaa atttttatca atgattatcc tgaaaagctc accaaattaa
      721 aggctgcggt tgatcagggt gatattgcca ccattcgttt tatcgctcac gatattaaat
      781 caggaagttt gagcatgggt ataaagccca tgtccaatat ctgcgaaatt cttgagcgtg
      841 atagttcgaa aatgaataaa gaggaactga aagaactttc agaacgattg gctgcggatt
      901 attttcagat ctcaaaaagt tatggggatt accttaatat taaacgctga aaagagaaag
      961 agagagtcgt caggcggctc tctctcgcaa ggcggtcaag ttattagagg acgggctgga
     1021 atggactcag tctcggcttc aggcaactca cttacaaatt ctggaacacg gcgacggcgc
     1081 cgatcatggc ggagtcgatc atgagtttta ccgagaataa gtttaagggc ctcgaagctc
     1141 gagtttatgg ctcgaaagag attgcgatct tggcgtaaag tcttataggt ttttgcgctg
     1201 atgcctgatt ttagggttac ttcacactga taaatgggat gacgattggc cgtttcctgg
     1261 cgatcttttt cgatccgaac ggcgatgtgt aaatccttgt ccggttgaag gagcttaccg
     1321 gccaaaagct ccgttttttt taaaataaaa tcctcagcat aagctgaacg ctgaacgtct
     1381 ttaaaaacta actctgtacg cataatttac tccatatcga tattgaattg gtatcctagt
     1441 ccgggaattg ttttaatata gtctccatgc tggcccaact tctttcgaac tgtgtatatg
     1501 tgtttgtcga tagatctctc attaatgtga acagtgcctc cccaaatgga gtcaatgagt
     1561 tgatttcgtg taaagatgcg gcccggtttt tgggctaagt gcaaaagaat gcgatattca
     1621 ataggcgtca tgttgatgag ttcgtgcttg tcctccttga tggcatagac cgaataagtc
     1681 aaggtgtcga aacgcaaatg ccctcgttgc aattgcagtt cattgcgatc atgaagcaaa
     1741 gattttttaa gttttgattc aatgcgtgct ttaagctcca gaggcacaaa gggttttgta
     1801 atatagtcat cagctcctag gctgagacct aaaattttgt cggtaacgcc ggttttcccg
     1861 gtcaagaaga tgaccggaat gcgagccgtc gcggggttgg tttgcaggtc attgcaatag
     1921 tcaaagccac tcccatctgg gagcatcaca tctaaaagaa tcaaatgaac ttcgcgactt
     1981 tccaaaaatt ctttagcctg cttgagtgtc gaagcaaaaa ataaatgaag gtctctttca
     2041 agggaactcc gtatcatcgc ctggtactcc actgaatctt caaccaccaa aacatttggt
     2101 ataaaagctt gaacgtgact gtctggtgct ttttcgcttg tccgatatcc ataatccctg
     2161 tatccactta caagttgatt gttaccatgc tgattagcat ccgtgttatt catctaaaaa
     2221 tcctccatgg tcaggtggtc aataacctaa aacatctcaa aatcaacaat tgtttcgtgc
     2281 ttttcgcccc tattccaata tttcatttga gcgctcaggt cgccgatggc gctgtccaaa
     2341 gcggccgcaa tcacttcgtt ttcacctgaa gca
//
LOCUS       DATBTE010000137        33403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1384627, whole genome shotgun sequence.
ACCESSION   DATBTE010000137 DATBTE010000000
VERSION     DATBTE010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 33403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33403
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384627"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VIG33_05060"
     CDS             complement(<1..730)
                     /locus_tag="VIG33_05060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY8270235.1"
                     /translation="MKSPNPLIIFVAVLASLLEIIDTSIVNVALSSMMGNLGATLEDI
                     SMVITGYAIANAIVLPISAWFSERIGRRKYFLGCIAAFTLTSVACGLAPNLLTLVIFR
                     ILQGLFGGALLPTSQTLIYEQFPKEKAGIAGAIFGMSVMVGPTLGPVMGGYLTDNFGW
                     RSIFNINLPLGIIALVVGMGVIYDRVSQDGEVQKEKPALDWVGFALLVLGIGCLQFVL
                     ERGESSDWFSSSSIVINSLISAVCL"
     gene            complement(730..1578)
                     /locus_tag="VIG33_05065"
     CDS             complement(730..1578)
                     /locus_tag="VIG33_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEY8270236.1"
                     /translation="MNNKKKILLSTGLLAVLLGVYFVIEHVMYVTTDNAQVEAPVVML
                     AAKVPGYIIDVNVVEGQRVKQGDILVEIDRRDYENTLKQVQSELAGLESRKKDAERNY
                     QRISQLYKQNAVSSQQYDTATASYAELKSKYEAITAQVDQAKLNIENTKIKAPVDGSI
                     AKKSVEKGQLASAGVPLIGFVGDQERWVMANFKETEISGIKIGSPVDIDIDAIGSKSF
                     KGRVVALSSATGSTFTLLPPDNATGNFTKVVQRIPVKIKLENLTNGDLEMLRAGLSAY
                     VKVRKN"
     gene            complement(1598..2941)
                     /locus_tag="VIG33_05070"
     CDS             complement(1598..2941)
                     /locus_tag="VIG33_05070"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY8270237.1"
                     /translation="MRVFFLIVIHFLSLQCFADNLTGSSTNNSIESLTLQQAIRESGE
                     HSPRLQKAKAMQEESSWKKIEALNSFLPTLTAQANYLFNKKYLLTDVRLNPSQPEVSI
                     AQVIPTTLFTVTGTLPLFEGRAGLNRYSSASSFSSASQKEYAWTEFQISREVILQFYK
                     TLAAKELLQVAEQNLKTLQDHLKDVNLFKKAGISTNYDVLRVEVQVSEAESEVLNTTD
                     NLSAARNKLTEIMGLEFDQRELVGTWPTFNAKKTENLIKAKLTARQDLQALELRSEGA
                     RSLSHAASSYWIPRLALFGQYQYYNNRDDHYDSWDKYREAYQVGLSMTWNLFDGMTSI
                     ARDHESFQQSVQSDKSLVQARLKAQQDLEFWKRKYLYFGTVFRARQSDVLKAEESVRL
                     ARQGKKVGTRTDTDILDAEVELFRARAGLVNAQMGALESLVNLELTTGDTLIGLN"
     gene            3044..3550
                     /locus_tag="VIG33_05075"
     CDS             3044..3550
                     /locus_tag="VIG33_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY8270238.1"
                     /translation="MKNSKKKNSTKKTTKKESAEITLHPALEKHLGYCLYKAALKFRA
                     MIDHMMVQEKLIAPQFGVLLILREIEGLNQINLGLQMGIDKATIVKLIDGLERAAYVT
                     RVSSETDRREKFLQITPRGRKLLEDLKPRMKDLETEFLKPLDPEEKSILLNAIPKLMK
                     PKREQEKI"
     gene            3531..4406
                     /locus_tag="VIG33_05080"
     CDS             3531..4406
                     /locus_tag="VIG33_05080"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HEY8270239.1"
                     /translation="MSKKKFNKVNIEISNICNLQCNFCPPVQREKRLMSLEIFNSVVK
                     QVAPLTEQVTLHLMGDPLVHPKLNEFIAVCERHKVPIFLVTNGVLMNAVKEELLLHPI
                     FRQVNFSLHSFHDNYGDKNPSVYLDKIFKYSERALIERPELYINYRLWNLHEPRGVGA
                     QNRSILERVCEYFEFKFEQTIDVRKNKSVRIKNRLHFHFDTEFTWPALDLPLLGERGT
                     CYGLSSHFGVLADGTVVPCCLDKEGKIPLGNVQEQSIEAILNNERAQKILQGFKANKL
                     LETLCQRCQYITRFN"
     gene            complement(4419..5300)
                     /locus_tag="VIG33_05085"
     CDS             complement(4419..5300)
                     /locus_tag="VIG33_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270240.1"
                     /translation="MQSLNVQKQELDKLIDQQLSNEFDGIILNDFCAKAESSAKYLLT
                     SLKYFFGQPAFVSRSLEILVHQKISFSKTVSGEGCAHPVKILIEGNSSQSKLQFSLTD
                     LCSIFLPEHKFEITKDWQKTLRENLQVLNSDLKGLCERKIAVSIGGGEIKAPGLDFWG
                     QIEMTPTHRFYAQAYPILKHIVSKGVPEISIAARWGSDSPYQRFLKSDHWSLFSFRIS
                     KRQQVDLAQYTELKKIKLRCALLDLGFLENGYGKVAKIKELISIEIPSDSSTDKISTI
                     QLDFFAKVLKQMSELVK"
     gene            complement(5362..6870)
                     /locus_tag="VIG33_05090"
     CDS             complement(5362..6870)
                     /locus_tag="VIG33_05090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8270241.1"
                     /translation="MNNSRRKFLRHMLIGTTGFVARNSFAGVFLSPKFEKCDCVVIGA
                     GMAGLIAARDLSFPKYARKGYKTILLEASGRIGGRIHTVEDPRFGGPLEMGAEYIHRD
                     PGTVALWNEVDKYKPKIEKISRMFKGLMYYEGWEDHLKKYYALVKEWNLYDILTFSHR
                     IDSYRGPDISAKQWIERQRFDRIGRNLVDLYFTGHVPGSLAEMSLKGFISDRISEQEM
                     GWNEYQFEDGYSDFLNQLVLGVDQHRGKQIDIRFHSFVKYIKYGKSGTEVHTTDGRIF
                     LAQAVILTASVGMLKSGDICFNPPLPQDKIDALNCMGMGDEAKIVLKFNKRFWPKDAV
                     FINRIDNQREMSRTYFVPFVDDPAKDKVLCILFAGDEADKIKNMTDMEVIQALCRDFD
                     KMFPSSAPTFNLLEKDRVTQVITYLRWQWSNDSFSKGADSFLKVGFERSIPIEKVRKA
                     LAHPASTPGLFWAGEATVSSEYTQPCSTHGAHFSGARAAIEVGEYLRTKLIV"
     gene            complement(7079..7285)
                     /locus_tag="VIG33_05095"
     CDS             complement(7079..7285)
                     /locus_tag="VIG33_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270242.1"
                     /translation="MNKLSILLVVGSVLTFSGVFALLTKPLPAPSSMTRQEVPPRPFH
                     RGVASAGEGFSAQPAIESVRSQKQ"
     gene            complement(7576..9825)
                     /locus_tag="VIG33_05100"
     CDS             complement(7576..9825)
                     /locus_tag="VIG33_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HEY8270243.1"
                     /translation="MKFRFPIVIIDEDFRSENASGLGIRALAAAIENEGMEILGVTSY
                     GDLTAFAQQQSRASAFLLSIDDEEFGGGSPEETEEALKVLHAFIEEIRFKNAEIPIYL
                     YGETRTSRHIPNTILRELRGFIHMHEDTPEFVARHIIREARSYLDSIAPPFFRALLNY
                     VQNGSYSWHCPGHSGGVAFLKSPVGQMFHQFFGENMLRADVCNAVDELGQLLDHTGPV
                     AASERNAARIFNADHLFFVTNGTSTSNKIVWHSTVGPDDIVVVDRNCHKSNLHAIMMT
                     GAVPVFLTPTRNHYGIIGPIPKEEFSPESIQKKIDNNPFIKNKKRKPRILTLTQSTYD
                     GVIYNVETLKQLLDGKIDTLHFDEAWLPHAAFHDFYKDMHAIGKDRPRAKESVIFSTQ
                     STHKLLAGLSQASQILVSESETKKLNRHVFNEAYLMHTSTSPQYSIIASCDVAAAMME
                     EPGGRALVEESIVEAMDFRRAMRKVDEEWGQDWWFQVWGPEDIADEGIGSRENWILQP
                     NDKWHGFGNLATDFNMLDPIKATVITPGLDVGGDFAEKGIPAAMVTKYLAEHGVIVEK
                     CGLYSFFIMFTIGITKGRWNTLLTALQQFKDDYDRNEPIWKILPQFAAANPHYERVGL
                     KTLCQQIHDTYKANDVARMTTDMYLSDMQPAMKPSEAFARMAHDEIERIEIDDLEGRV
                     TAVLLTPYPPGIPLLIPGERFNKTTVDYLKFARTFNLQFPGFETDIHGLVSQIENGVR
                     KYYVDCVKI"
     gene            9934..11025
                     /gene="argC"
                     /locus_tag="VIG33_05105"
     CDS             9934..11025
                     /gene="argC"
                     /locus_tag="VIG33_05105"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY8270244.1"
                     /translation="MHKYPATNPLSTSPHGFLGTSGSISCSIVGARGYSGLETARLLL
                     QHPYAQLTHCFATSEFKLSNFISNSGAVQVQCLPDSEIMNHLTDVVFLATPANVSLSL
                     APKVLSAGKKVIDLSGAFRLKKNDYQKWYGFAHTEQEALTEACYGLAPWVGPASEERL
                     IANPGCYATAIALALIPLLKNHLIAENSIVVDAKSGTSGGGKKAVENLLFTEVDGECL
                     PYKVGCHQHYPEIIEALEAFSGKKIEAHLTTSLLPIRRGIIAGVYAQLHEGIGLQEVA
                     AAFAQAYGDYPLASFGPLKTEPTLLSLKKVVGTAKTHISFEVVGQRLYVFSCIDNLLK
                     GAASQAIENFNRISDLPISTGLSHLEALT"
     gene            11022..12218
                     /gene="argJ"
                     /locus_tag="VIG33_05110"
     CDS             11022..12218
                     /gene="argJ"
                     /locus_tag="VIG33_05110"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEY8270245.1"
                     /translation="MTPGPGVHRTPLPKGFLASGVNCGVRRYRPDIGILISEVPSVAA
                     GVFTLNECKAAPVHYSQHLLPADNIKAILTNSGQANAATGAEGVEKNLMMISATAKSL
                     NCDMNQVLVASTGVIGVQLDTDKILPSIPELVSRANDTAESFATAILTTDLVPKTVTA
                     VVELSTGSVRITGISKGSGMIHPNMATMLGYILTDAKITKEHAAELIKKTADISFNMI
                     SVDGDSSTNDCCFLMANGATGVAIKTAEDKEKFEAIVQDIGVFLAKSIAADGEGATKL
                     IEVEIKGSDNLALAKKAARGVTLSPLIKTAVHGEDPNWGRILARLGAEQVPVQQLEKM
                     TLRLQGVLIFEKGQPVAFARDEVKALLKQVQVKIEIDLKSGPHSATAWGCDLSRKYVD
                     INTEYS"
     gene            12215..13039
                     /gene="argB"
                     /locus_tag="VIG33_05115"
     CDS             12215..13039
                     /gene="argB"
                     /locus_tag="VIG33_05115"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY8270246.1"
                     /translation="MKKKILIKLGGSSLENPATVRELVALTSGYRKKNYDIILVHGGG
                     PAINQELTRRGIEWKFINGQRQTTPQMMDVIEEVLAKQINSMLVSVLLAAEIPAVGIS
                     GIQDKTLLCSQANSELMQVGKVESVNTAALEKILQLELGPTPVIAPLGAGLHGEKYNI
                     NADWAAAKIAIALQAEKLIFLTDQNGILDQDKQLVHRVTPQLINKMIEAEAISGGMYT
                     KVMTMMTALSAGIPQVRVLNANVASQLLNNDEIGTLLSNDLPPARKEVTEWNQKLN"
     gene            13018..13491
                     /locus_tag="VIG33_05120"
     CDS             13018..13491
                     /locus_tag="VIG33_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270247.1"
                     /translation="MESKAELKNDSHSRVLVLRQLIREGTASTQEDLSKALKLKRFNV
                     TQSTISRDLRRIGAIKTTNMEGQTIYKLPETHQTLPPGGNHSLAGHLIDIQSNESMII
                     LHTSPGSASLVARHLDNMRSALGILGTVAGDDTIFIAPASIKKISSLIRRIKEEI"
     gene            complement(13500..14951)
                     /locus_tag="VIG33_05125"
     CDS             complement(13500..14951)
                     /locus_tag="VIG33_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homospermidine synthase"
                     /protein_id="HEY8270248.1"
                     /translation="MTKSTSFVPPSANYGEITGPIVMIGFGSIGRGCLPLIERHFKFD
                     KSRLVVIDPSDRDRKLLDERGIRFIHSGLTAANYKEILLPLLKNGSGTGFCVNLSVDV
                     SSLDVMNLCRDIGAHYIDTVVEPWVGFYFDDSKGASGRSNYALREGLLKEKATNPGGI
                     TAVSCCGANPGMVSWFVKQALVNLANDLKMDYEMPKTREQWAKLMQKVGVKGIHIAER
                     DTQRAKNPKPMNNFVNTWSVDGFICEGMQPAELGWGTHEKSMPETGRRHEFGCQAAIY
                     LQQPGANTRVRTWCPTPGPQYGRLVTHNEAISIADYFTVGQGANAEFRPTCHYAYHPC
                     NDAVLSLDEMFGRAGARQPEQHILDENEIVDGLDELGVLLYGHEKNAYWYGSQLSIEE
                     TRLLAPYQNATGLQVTSAVIAGMAWALENPKAGVVEADEMDFKRCLEFQLPYLGPVKG
                     YYTDWHPLQDRPGLFPEDIDTQDPWQFRNILVK"
     gene            15100..15327
                     /locus_tag="VIG33_05130"
     CDS             15100..15327
                     /locus_tag="VIG33_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270249.1"
                     /translation="MPKRRTKTSSKYRGVSFQKASGKWRAGIEVEGIHRNLGDFTKES
                     DAALAYNSAARKYFGKIAFQNSVGRKKVPRK"
     gene            15406..16725
                     /locus_tag="VIG33_05135"
     CDS             15406..16725
                     /locus_tag="VIG33_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8270250.1"
                     /translation="MLTTRISKQIDKAILCLIGLFIFFTAQSSFSANGSNEIKIWKDS
                     RYNLPRKSGVVLFKLKKNKQLNDLQSSFLIPLAEDIGLTDTRLLGSSSIYELNFQIPL
                     ATMSSAAHHAHHVEEAFANKLMASGLVEFAEPDYIFTPDTVTNDPNIADQWYLQKISA
                     PAAWDVTTGSNNTQVLVCDTGIMSSHPDLNENILNQGHNFVDDSGNTEPSGNPHGTLV
                     AGLIGAKGNNGIGMAGLNWNIQIIPGKISNDPKGAAQSSTMVKCIQWAIDHNIRVVNL
                     SYSGTESDAIRDASKTLFENNGLLIYTIGNAGVEVTRPYNPYALTVGASDDYDFKPPW
                     SNTGEALDLVAPGVWIYSTDGNGAYKNVQGTSFAAPLVTGTVALLISLNPERPMKELR
                     QIIIDSAKDLWTPGKDKAFGFGRLDISKAMEIYRSSAAVANTTVRDN"
     gene            16981..19968
                     /locus_tag="VIG33_05140"
     CDS             16981..19968
                     /locus_tag="VIG33_05140"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEY8270251.1"
                     /translation="MITRFFKKCAIALTFAVFILCFQNCARETSLSSSSREAASPPAE
                     IILNTDSKDEVNTSDYNLTFSTKIKNNDPMTSITCQLNNEPSQDCSSGSIVFPSLADG
                     DHQATISTVTSTGAITQMKRVFRKDTTGPVLTVTNNISAVTAKTSASFKFFATDALSR
                     VRGYKCALDGKAFSRCSSPLNRSGLVSGKSYSVSIRASDRAGNLSKIYSFSWTVKGGV
                     PTAVLSSNLPSPMTSSTQITLTFTGTNAASFKCSLDGAVSTVCTSPQTLTVSANKLHQ
                     FSVTPISATSVLGAASSYSWTVDTLPPTSPTLLANIPTSTTSKSATFNFSSTDNLSGV
                     THYTCSLNNAAPITCTTPYNLTVNNDGAYTLAVRAVDSAGNTSAPGSFSWTVSATMPP
                     PADGKTLFASNCASCHNGPDTFDSTKTTKTNRTSAQISASINSLAPMNTIAGLKTLTT
                     VQIDAIAAYLKTTPPPPALACSTPFAPERTPLRLLSNLEYDSIAADIFLSKRKPSLDT
                     KFDVPALGSSGFSNTSMTSETSSPPISDLTVEKYWTAAGALANEVIANKSQSGSAYSA
                     LASCAVGVSSVTDTCYNSILRNVTLKVWRRPVSETSTNNEFSRLMPILKSGGSFDVGF
                     RDFLKALLISPNFLVVSFTPGTTVSTTSQVFTLNNYQLASRLSFFLWQSAPDDTLLGL
                     AANGTLSNTTTLQSQVLRMLKDPKGKRFASVMTNEWIGANQILGLGLTTVDTATLNSM
                     VAETQFMFEDIVNTDSSFLNSISANYSFLNKVLADYYKVPFNGAVSTQFYKTSLDSTP
                     RRGVLNQAAFLIATSGAPNQTHPVSRGKIIARKIACYDIAPPPANLDTSLPANIPANS
                     TPAEILAIHTQRAQCAGCHNSIDPHGLPLETFDSRGIWRTNYLDIGGRSIDQSGKLPS
                     GETFSSTQEFMSTLINSSNVRSCLVRNVMAAGLARKVASLNDQCNATQISELSMNTNS
                     KLSDLISNIVISRQFRMQTTETP"
     gene            19965..21332
                     /locus_tag="VIG33_05145"
     CDS             19965..21332
                     /locus_tag="VIG33_05145"
                     /inference="COORDINATES: protein motif:HMM:NF019222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1552 domain-containing protein"
                     /protein_id="HEY8270252.1"
                     /translation="MSNFKISRRKFMSSTGMIFTIPLLESLFPLSAEAANDPRRYVIF
                     YFPNGTYNRSDKPIWATRDGALSSSNTSLALSPFSANYGDMISINYLKNDANFGLAIN
                     DDHARNAAAYLTCASSLSSSMISFENVLADKFGKAALVLSGGVTSADLPPDSYISYRN
                     GKGNMGISNPGDLYRNLLNQVIPNAGPTPTPTPTVNNGKSILDATIADLNSFMKTLGR
                     SDKAKMEEFLTSVRALELKVASTAPTPVPAPMTGSGTGCVKPTLDSNLDSANSRDAAL
                     YLPKFYAMNDMIKIAFACDITRSISVMVDTETTTRTYAKPPSSLIYNGADISGGYTSH
                     IAISHASGLSTSGYNLAVTRDRVLMQIPMDLVNKLKSANDPSGSRILDNTIIQAGFGV
                     QDGNHSASNNQRPLILAGGRNMLNLGTSRNLSSYQLKDLYYTISNKIGAGLTNFQGSS
                     TLISL"
     gene            21759..22451
                     /locus_tag="VIG33_05150"
     CDS             21759..22451
                     /locus_tag="VIG33_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270253.1"
                     /translation="MQASFELRNSMKLTNSILKTLYSFCFLFLVTVAANAQCPQQLQS
                     DTLSTESFLRDLRKHIDTENENTIPYYVLGLDEGLTAFYNKVKEDTHHQTLYYDTLYE
                     FSLSDHPLKSKLLKEVKLQKKYHSVEEWMPVFEKTMQNWVVAAPNRKTIYFNLDRFDF
                     KKYQQFSSQIQDTIGEKKASSKWTNFTNAEMKFILSDKKIFNKIRWFVNNRELTSSEI
                     KRTFKDVAPQLF"
     gene            complement(22508..22972)
                     /locus_tag="VIG33_05155"
     CDS             complement(22508..22972)
                     /locus_tag="VIG33_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270254.1"
                     /translation="MKYLILLANLIFSIQVWAISECTGRNADGSWVTVHINTTGATGF
                     PDQGEVVIENKENRFGYHFSHDEITQFFEYDEPANNSAMVGLAAYVNKESPVAVKYNG
                     PNFVDMDLKSVIETDKAKLIKGNFLRIWKGPGHAANDQFQLTNIACSTWLNI"
     gene            complement(23003..23851)
                     /locus_tag="VIG33_05160"
     CDS             complement(23003..23851)
                     /locus_tag="VIG33_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270255.1"
                     /translation="MRILSLYLGVFVILASSGGVAGWDHGNAGDAVASEFILTGRDVL
                     QRLELLFNNNKPAFDTVNLRVAITATEVVSEDHTLLDGYERDAVNYYPTKRLIKVNRS
                     RWMDLRRSTETKSRLRLVLHEYLWISGVDDTNFVHSEYLIELLNIGNYSPSIWWNPVN
                     PANYVNASLSFGSEGCAFQPGKLNVKLSDETLVMEPVGHCPEDVYRKVQIVKSTGITP
                     ASSHARGFFHKYEMTVFDKAQTVKGAMTFEPEWGACLLPENGACQVSGKMTLGGIEFV
                     FWFLRN"
     gene            complement(23848..24606)
                     /locus_tag="VIG33_05165"
     CDS             complement(23848..24606)
                     /locus_tag="VIG33_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HEY8270256.1"
                     /translation="MKESTLIEQMKRTFAERCRKNSSYSLRAFARSLGMDSSTLSAIL
                     KGKRPLTLKTARKIVEGLNIRNPVEAQALIVSTFASDEAAAVPSYTELAMESAEAISS
                     WQHFAILALLEIKDFKGQERAISERLNIPFGIVSECLNRLEKLGLIVKKKNIWQLTGK
                     NMATPSQIPSAALREGHRQYILKVLHSLEVDPVEVRDVSGITMAVSKSRLAEAKVMIQ
                     DFRRRLSTYLEEGSRDAVYRLNIQLFPLSQEKKP"
     gene            complement(24619..30495)
                     /locus_tag="VIG33_05170"
     CDS             complement(24619..30495)
                     /locus_tag="VIG33_05170"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEY8270257.1"
                     /translation="MSETAKATGPVQDFNGAVPEPIGKVDESFSQLRIKFPNSMNKSK
                     NSQKGPFTVSCTPIVEGFPSWADNDTLWTYNFKAKDEYSSARLVGGTQCNIVQTEDLT
                     SVDNKVWVAGTINYSVIVQGPNVEEVYAAHGFQDTLREHEPVILILFDGPVDRSKFFA
                     DQSGFLSYLSANAPSEKMPLVAVPEDQSEKIFSHFASNRYFNVEYKDQNWVLATIQQN
                     LIPGAQVNLTIQNQSSAANGSIHSEKKFTKEFSVRSQFQAQVQCANPSAKNATCLPRS
                     PISVALNGRAKWTDIKESYIEYIPYKSADKKLVRSYPELNQEVSLWESFLNTLSDYFP
                     FLAKYSDTVVDSIIFKVNIEPETQAKVVLPKGLKDIEGRILANAIAEFHIRIGTMSEV
                     IHVPQTISFFEKNVPNLYLPVGVVNLNQKLSIRKTGTNAKIWEPIQDVPSMINVIRGY
                     ENRGEYRNTPTYTSPLDAMGVKNTVIGQSLTGKKNRPNFLQFPFGEAGKAPSSGFYAI
                     EISSPTFESGRSNSEDEQYYNPKYVLAQVTDLAVHLKKGQTNTLAWVTRLSEAQPVAN
                     ADVEIYNCLGQQVKTLKTDGSGLVRFPNQEWAKDCKAPQYSYSPYFAKDAFYIVARQA
                     DDLALIHSSWTSPNTYALSAPGIEWFYSDISENQPHFHSVIGVNLVKPGQQVPIELIA
                     KLPLDKGFTEVAVNKLPTVARISSADDEDTYYELPLSWKNGVADIVWNVPNDSSVKLG
                     RYYIKLTGTRDGESINVASGDIEVAEFKVPLMSGLISFPTQALVQPSSIPVSAVIRYA
                     NGVGAKNLAADMSYYFEPTSIQNKMLSDFNFGSGAVKLSADESAESTEVLPRGSRPAT
                     IQGLSTAADGSLTKDIALENAADGRSVAEVLKTLGRPQKLVVRVRYQDQMGEYQTLSQ
                     AKDIFNSHEYVGTSLLSGNRSEARLRAAVMDVNQKNITDLKNLEIKVIRVETKVMGEE
                     LYGGLIKNTLERELKPVRWHETCVVQKQVVNCPVGTLKAGNYAFQVTSLTTKQAANTL
                     FKVDSDGRVYGANDYYNFGDDPGNKQLPLALNKKTYKDGERAVVSFPAPFKNCRALVT
                     IERSEVMEATLVSNACEKGFVEIPVHADLAPNAFVSIYAITGRAQTAALKIGETDLGR
                     PTYRLGYANMRVNWSHFKSKVTVKTNKEKYEPGETVDVQVNVQPEDGKLAGGTVTLVA
                     IEEKILELKANNTYKVLDALMQLRGHNVETISPLERVETVTADNADLPDEDSRKGGDE
                     GGDGSAKSDFKRKLFNALVAFQPSVPVVNGVAKFSFQTNDSLTRFKIFAIATDSAQKF
                     GTGEAGYLTEKDTQVYSNIPSVAHSGDSYPIKVTVQNNSGKDSKFKTEVEVVMRDSNG
                     KVIGTKTLTQQSDVKSSASETVDLGEFTVDEQAHRIEYVIRIYDENGKVVDAMEPDAQ
                     VILPSVPLAIHTSYLAQMESGAFSQELTKELNALPGKGEIRVSVSNSLVNGAVKQIQQ
                     RMSRDTFIDFFIESQFYKALLNSTESKPEELKRVLVSLLGSTDTNGFVKYYPQAQRGS
                     VWLTASILNALQQEPWALKLMPAALIGKLKGAVSQVLSKSVNPDYVGKTPMDWMTAQT
                     VMGRSAFAFADPKLQALAKSVSTSLAAELQQNPLAFGPPVEKWSNSDLVNFWLLQVFA
                     TPTTAATSPIYAQLLGPSRLVYTGNMAQLSGSPSYSYFYSDETMETANLLLGHALLRR
                     DKNVARSLAIGLVNVNTKAWYNTSTMMFVAQSLKTFGRAYEAEVVSGSTVMAISEQQV
                     SKTVEWSQSTTAEIAAPWISTKATVQVTHSGQGQPWVGIQGLSAVPLIAARGQGLSIE
                     KELHNVTRESGFQAGDIVEVTLKINSTGNVNNVALQDPIPAGSNILSDAYGDYSAGQK
                     SYSGYKFYFEYLDSGITTVKYQFQLNNPGTFKLPPTHAEALYMPSVFGEAPNAALTVK
                     "
     gene            30768..32918
                     /gene="pbpC"
                     /locus_tag="VIG33_05175"
     CDS             30768..32918
                     /gene="pbpC"
                     /locus_tag="VIG33_05175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEY8270258.1"
                     /translation="MHKNIKTTFLAALCGVLTLSLISWIATPSADKIIRLSLGSDRVL
                     LAANGDLLQNLRTDFKKRRLAWHPLQRFPESLQRAVVSAEDQRFFSHWGVDPLSLGRA
                     LLANIQGKRLQGASTITMQLSDLIQENVLLHNQVIKKGSVFHKFAQITRAFFIELKWS
                     KKEILEAYLNLIHLRGEFQGVPALTYAYLKKDPLALEAAESLVIASMISSPNQSLISL
                     KAKACLLNRRQDHATPPSCEKVNAAAEFFFNKSPAMPVSPNYAPHLARRLFNEFAEDA
                     ILTSTINSDLQKKVSAILEKNVYRLKDSHVHDTTAIVIDNRSGKVLAYVGTVSSSENP
                     HVDGILSYRQAGSSLKPFIYAKALETKTLTAASILLDDPTAISWNGEVYRPANYDKHF
                     YGPVAVREALGSSLNVPAVKTVTIIGLHETYQVLQSMQLSHLKEPDFYGVSMALGAVE
                     VRLDELANAYRMLANGGAWSPLRFTQKDIAKDFKPKSVFSPEAAYIIGSILSDPDARA
                     IGFGWETPLETPFWTAVKTGTSKDYRDNWCLGFSEKYTVGVWAGNFNAEAMKKVSGVS
                     GVGPSWYEIMTTLHSQERSSPPPMPSPVIAKDIRHQWASHVHKEYFIKGTEPTQEVIE
                     PALDKRIQFVFPADGSVLVKDPHLDQQYVALFVRFKGSVPKKSQLLWDGKPLGEAVSP
                     FKIPDPASGDHELAVASKDGKILGKIRFTIHGAE"
     gene            33062..>33403
                     /locus_tag="VIG33_05180"
     CDS             33062..>33403
                     /locus_tag="VIG33_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270259.1"
                     /translation="MEILIAFGLLAILGLGISSMIQLGFMGQKGIEKDYAINNISEQI
                     RGILGDPKACKNTFGGQLVGASAQPITQILDGSTPPVSVYSSGSSTVYESRNIKISAM
                     NLKTDSTIGNVP"
BASE COUNT         9183 a         8211 c         7049 g         8960 t
ORIGIN      
        1 gaaggcaaac agcagaaatc aagctattaa taacaatcga cgaggaagaa aaccaatctg
       61 aagattcccc ccgctctagc acgaattgca agcagccaat tcctaatact aaaagtgcaa
      121 aacctaccca gtccaaagca ggtttctctt tttgcacttc gccatcctgg gaaacgcgat
      181 cgtaaatcac gcccatgcct acaaccaaag cgatgattcc cagaggtaaa ttgatattaa
      241 agatggatct ccagccaaag ttatcggtga ggtagcctcc catcaccgga cctaaggtag
      301 gtcccaccat cacgctcatc ccgaaaatcg ctccggcaat acccgctttc tctttgggaa
      361 actgttcata gatcaaagtt tgtgaagtgg gtaaaagagc accaccgaaa agcccttgaa
      421 ggattctaaa aattaccaaa gtcaaaagat tcggcgcaag accacaggcg accgacgtca
      481 aagtaaaagc cgcgatacaa cccaaaaaat atttacgacg tccaatccgt tcactaaacc
      541 aagcggagat gggcaaaaca atggcattgg caatggcata gccggtaatg accatgctga
      601 tatcttcgag ggtcgcaccc agatttccca tcatcgaaga caaagccaca ttgacgatag
      661 aggtatcaat gatctctaga agtgaagcca ggaccgcaac aaatataatc aacggattcg
      721 gacttttcat tagttctttc taactttcac gtaagcagat aagcccgctc gtaacatctc
      781 caaatcacca ttggttaaat tctccaattt aatttttaca ggaatgcgtt gaaccacttt
      841 ggtgaaattt ccagtcgcgt tgtccggtgg caacagggta aaggtggatc ccgtggccga
      901 acttaaagcc accaccctgc ctttaaaaga tttgctgcca atagcatcta tatcgatgtc
      961 cacagggctg ccaattttaa ttcctgaaat ttcagtctct ttaaaatttg ccatcaccca
     1021 gcgttcttga tcgccgacaa agccaatgag ggggacgcca gcggaagcga gctgcccttt
     1081 ctccacggat ttttttgcaa tggaaccgtc aacaggagct ttaattttcg tattttcaat
     1141 attcagttta gcttgatcta cttgggcagt gatggcttcg tattttgatt ttaactcggc
     1201 ataagacgct gtggccgtat catactgctg tgaactcacg gcattttgct tatagagttg
     1261 agaaatcctc tgataatttc tttcagcgtc ctttttacgg gactctaaac ccgcaagttc
     1321 gctttgcacc tgttttaagg tattttcata gtctcggcga tcgatttcaa ccaggatgtc
     1381 tccttgtttc actctttggc cttccaccac attcacatcg ataatataac ccggcacttt
     1441 tgccgccagc atcaccactg gagcttcgac ttgagcattg tctgttgtca cgtacatcac
     1501 atgctctatt acaaagtaca ctcctaagag gaccgctagt aaaccagtgc ttagtaaaat
     1561 ctttttcttg ttattcatag aatcctcagc tttattttta atttaaacca atgagcgtat
     1621 cgccagtggt gagttctaga ttcactaagg attccaaggc tcccatttgc gcattcacga
     1681 gccccgctcg cgctcgaaag agttccactt ccgcatctaa aatatccgta tctgttcgag
     1741 ttcccacttt ttttccttgg cgagccaagc gcacggactc ttcggcttta agcacatcac
     1801 tctggcgagc tctgaacacc gtgccaaaat aaagatattt tcttttccaa aactcaagat
     1861 cttgctgggc tttcaaacgc gcttgaacca agctcttatc cgactgcacc gattgttgaa
     1921 aactttcatg atcacgggca atcgaagtca tgccgtcaaa aaggttccag gtcatactga
     1981 gacccacttg ataagcttcg cggtacttat cccagctgtc ataatgatca tcgcgattat
     2041 tatagtactg atactggcca aacaaggcga gacgcggaat ccaataactg ctcgccgcat
     2101 gggaaagact gcgagcgcct tcactgcgca attccagcgc ttgcaaatct tgacgagcgg
     2161 ttaatttcgc tttgattaag ttctctgttt ttttcgcatt gaaagtcggc caagttccga
     2221 caagctctct ttgatcgaat tctaaaccca taatctccgt gagtttgttt cgtgccgctg
     2281 acaagttgtc ggtggtattg agaacttcag attcggcctc actgacttga acttcgaccc
     2341 gtaaaacgtc atagttcgtg gaaatccccg cttttttaaa taaatttaca tcttttaaat
     2401 gatcttgaag ggttttcaag ttttgctccg ccacctggag caattccttg gccgccaaag
     2461 tcttatagaa ctgcaaaatc acttcccggc tgatttgaaa ctccgtccaa gcatattcct
     2521 tttgtgaagc cgaagaaaaa gaactggctg aggagtagcg atttaatccc gcgcgccctt
     2581 cgaaaagcgg caaagtccca gtcactgtaa aaagggttgt cggtatcact tgagcgatcg
     2641 agacctctgg ctgagaggga ttcaaacgga catcggtcaa tagatacttt ttattaaaaa
     2701 gataatttgc ttgcgccgtc agcgtgggga gaaaagaatt gagagcttcg atttttttcc
     2761 aagaactttc ctcttgcata gccttggctt tttgcaaacg cggagagtgc tctccagact
     2821 cacggatagc ctgctgcaga gttaaactct caattgaatt attggttgaa ctccctgtca
     2881 aattatccgc aaagcattga aggcttaaaa aatgaatgac tattaaaaag aaaaccctca
     2941 tttgaactcc taaagtatgt tcggtagaga tgtgtaacac aaatcgtttg cattgcaaac
     3001 gatttgtgtt acaacccata aaatgaaaag tccgaagaag ccgttgaaaa attccaaaaa
     3061 aaagaattct acaaaaaaga caaccaagaa ggagtcagca gaaattacac ttcacccggc
     3121 tcttgaaaaa catttgggct attgtctcta taaagcggcc cttaagtttc gtgccatgat
     3181 cgaccatatg atggttcagg aaaaactcat cgccccgcaa tttggagtcc tacttatttt
     3241 aagagagatt gagggcctca atcagatcaa tcttggtctg caaatgggta ttgataaagc
     3301 caccatcgtc aaactgattg atggactaga acgtgcggcc tacgtcaccc gggtgagttc
     3361 tgaaaccgat cgccgtgaga aatttttaca gatcactccg cgaggacgta aacttctcga
     3421 ggatctcaaa ccccgcatga aagatcttga aactgagttt cttaagcccc tggatcctga
     3481 agaaaaaagc atcttgttaa atgcaatacc taaacttatg aagccgaagc gtgagcaaga
     3541 aaaaatttaa taaagttaac attgaaatca gcaatatctg caatctccaa tgcaactttt
     3601 gtccgccagt acaacgggaa aaaaggctga tgagcttgga gatctttaat tccgtcgtga
     3661 agcaggtggc ccccctcaca gagcaagtga ctttgcattt gatgggagac cccttggtgc
     3721 atcccaaatt aaatgaattt atcgccgtct gtgaacgcca taaagtgcct attttcttgg
     3781 tcaccaatgg agttcttatg aacgcggtta aagaagaact gcttcttcat ccaatttttc
     3841 gccaagtcaa tttttcgctc catagtttcc atgacaatta cggagataaa aatcctagtg
     3901 tttatctaga taagattttt aaatactcgg aacgcgcact tatagaacgg cctgaactct
     3961 atattaacta tcgtctctgg aatttgcatg aaccccgagg cgtgggcgca cagaatcgtt
     4021 ctatcctgga gcgagtgtgt gagtactttg aatttaaatt tgagcaaact attgatgttc
     4081 gaaagaataa aagcgtgcgc attaaaaacc gcctgcattt tcacttcgat accgagttca
     4141 cctggcccgc cctggattta cccctcttgg gggaacgagg aacctgttat ggcctctcca
     4201 gtcacttcgg cgtgctcgcg gatggaaccg tcgttccctg ctgcttagat aaggaaggca
     4261 agattccttt aggaaacgtc caagaacaaa gcattgaagc tattctgaac aatgagcggg
     4321 cgcaaaagat cttgcagggt tttaaagcca ataagctgct ggagactctc tgccaacgct
     4381 gccagtacat cacccgcttt aattagccct aaaaattcct attttaccag ctctgacatt
     4441 tgttttagga ccttagcgaa aaagtctaat tgaatcgtcg agattttatc cgtactacta
     4501 tcgctaggga tctcaatgga aatcagttct ttaatctttg ccactttgcc gtagccattt
     4561 tctagaaatc ctaaatccag caaagcgcaa cgaagcttta tttttttcaa ctctgtgtac
     4621 tgagcaagat cgacttgctg acgtttgctg atgcgaaaag aaaataaaga ccaatggtcg
     4681 gattttaaaa atctctgata aggcgaatcg gaaccccatc gggctgcgat tgaaatctct
     4741 gggacccctt tagaaacaat atgcttcaaa ataggataag cttgcgcgta aaacctgtgc
     4801 gtgggggtca tctctatttg accccaaaaa tctaagcccg gagccttaat ctctccccca
     4861 ccaatggaaa ctgcaatttt acgctcacaa aggcctttta gatctgagtt taaaacctgc
     4921 aaattctcgc gaagagtctt ttgccaatcc ttagttattt caaacttatg ctctggaaga
     4981 aaaatagaac aaagatccgt caaagaaaac tgcagcttgc tttgagaaga atttccttca
     5041 attagaattt ttacggggtg agcacagcct tctccactca ccgtttttga aaaagagatc
     5101 ttttgatgaa caagaatttc aagggatcgt gaaacaaatg ccggttgtcc aaaaaaatac
     5161 ttcagactcg tgagcagata ctttgccgaa gattccgctt tcgcgcaaaa atcattcaga
     5221 ataatgccat caaattcatt agaaagctgt tgatctatta acttgtctaa ctcttgcttt
     5281 tgcacgttaa gactttgcat ttcaaaactg ttacattcaa tttgctagtt gtccacagcc
     5341 atcaggctca aatattagat tttatacaat taatttggtc cgtaaatact ctccaacttc
     5401 aatcgccgcc cttgctcccg aaaaatgagc tccatgggtt gagcaaggtt gggtgtattc
     5461 actggacacc gtcgcctctc ctgcccaaaa taaacctggt gtcgaggccg gatgagccaa
     5521 ggccttcctc actttttcta taggaatgga tctttcaaag cccactttta agaaggaatc
     5581 cgcgcctttc gaaaaggagt cattactcca ttgccatctc aggtaagtga taacctgagt
     5641 tacacgatct ttctcgagaa gattaaaagt gggggcagag ctggggaaca ttttatcaaa
     5701 atccctgcac agtgcctgaa tcacttccat atccgtcata ttttttattt tatctgcctc
     5761 atcgccagca aataaaatac acagcacttt gtcttttgcc ggatcatcaa caaacggaac
     5821 gaagtaggtt cgagacatct ctcgctgatt gtcaatacga tttataaaaa cggcatcctt
     5881 tggccaaaac ctcttattga atttcaaaac tatcttagct tcatcgccca tccccatgca
     5941 attgagagcg tctattttat cttgcggcaa aggcggatta aagcagatat caccgctctt
     6001 aagcattccc accgaagcag taagtatcac tgcttgagct aagaaaatac gtccatccgt
     6061 ggtatggacc tcagttccac ttttgccata ttttatgtat ttcacgaagg aatgaaaacg
     6121 aatatcaatt tgctttcccc tgtgctgatc cactccaaga accaactgat ttaaaaaatc
     6181 actatatcca tcttcgaatt gatattcatt ccatcccatt tcttgttccg aaattcgatc
     6241 agaaataaat cctttaaggc tcatttccgc aagagaacca ggaacatgac ctgtgaagta
     6301 gagatccact aagtttctcc caattctgtc aaatctttgt cgctcgatcc attgcttcgc
     6361 cgaaatatcc gggcccctgt aagaatcaat gcgatgggaa aaagtaagaa tatcgtaaag
     6421 attccactcc ttgactagag cataatactt tttaagatga tcttcccaac cctcgtagta
     6481 cataagacct ttgaacattc tggagatttt ttctattttt ggtttatact tatccacttc
     6541 attccatagg gccacagtcc ccggatcccg gtggatgtat tcggcaccca tctctaaggg
     6601 gccgccaaag cgaggatcct ccactgtgtg aatgcgcccc ccaattctcc cagaggcttc
     6661 taacagaatg gttttataac cttttctagc gtatttcgga aagctcagat ctcgggcggc
     6721 gatgagacct gccattcctg ccccaatcac aacgcaatcg catttttcaa attttgggga
     6781 caaaaaaact ccagcgaaac tatttcgcgc cacaaacccg gtcgtgccaa tcaacatatg
     6841 tctgagaaac ttgcgtcttg aattgttcat atgattcaat cccttcattt ataatgaaag
     6901 aaatctatac tttgggcatt tcttgtaaat acaaactcta gaatgagaga gccgtacttg
     6961 aaggactgat agttttcgtg aaaaggaaag cccaagagaa aattagatct aattttgccg
     7021 gacgaaaatg tcactgggtt tatgttgaag ttttatacat atgactttgc tgtagtgatt
     7081 actgcttttg ggatctaacc gattcgatcg cgggctgcgc agaaaagcct tctccggcgg
     7141 aggcgactcc acggtggaaa ggtcttggag gaacttcctg tcttgtcata gacgagggcg
     7201 ccggcaaggg cttcgtcaga agagcgaaga ctcctgaaaa ggtcagtaca cttccaacga
     7261 ctaaaagaat agaaagctta ttcatctttt ttagtttatc tccttgtttg agaaaagcaa
     7321 gatggagcta gacctttgat agagctaagt acagagacca gggtctcata aagagacact
     7381 tgtcttgcaa aaactattca ttcgtactca aagacttatt ccttgaaaac tggccccctt
     7441 ctctgttgat ggccgaggga ttgctcttta gctctagcta tgcggccaga gtgtccctca
     7501 ctgctgtgac caaactcttt ctaattcgca aaatccatta agggatttca ttatcaacaa
     7561 tatggggggc cagtttcaaa tcttcacaca gtcgacgtag tatttgcgca cgccgttttc
     7621 gatctgactt accaaaccat ggatgtcggt ttcaaagcct ggaaattgca gattaaaagt
     7681 tcgagcgaat tttaaataat ccacggtggt cttgttaaaa cgctcccccg gaatcaacaa
     7741 aggaattccc ggaggatagg gagtcaacag caccgcagtc actcgacctt ccagatcatc
     7801 aatttcaatg cgttcgattt catcatgagc catgcgcgca aaagcctcgg aaggcttcat
     7861 cgctggttgc atatctgaca gatacatatc tgtcgtcatc cgagccacat cattggcttt
     7921 ataagtatcg tgaatttgct gacaaagcgt tttcaaaccc actcgttcgt aatggggatt
     7981 tgccgccgca aactgcggca ggattttcca gatcggttca tttcggtcgt aatcatcttt
     8041 gaattgttgc agcgctgtta acaaagtatt ccagcggccc ttagtgatac cgatggtaaa
     8101 cattataaaa aaggaataga ggccgcactt ctcgacgatc accccatgtt cggccaaata
     8161 cttggtgacc atagccgcag gaattccttt ttcggcaaag tccccgccca cgtctaagcc
     8221 cggcgtgatc accgtcgcct tgatcggatc caacatattg aagtctgtcg ccaagtttcc
     8281 aaagccatgc cacttgtcat tgggctgcaa gatccagttt tcacgggaac cgatgccctc
     8341 gtcagcgatg tcctcaggcc cccacacctg gaaccaccaa tcctggcccc actcttcgtc
     8401 cactttgcgc atggcccgac ggaaatccat ggcttccaca atgctttctt caacgagggc
     8461 cctgccgccc ggctcttcca tcattgcggc cgcaacatcg caggaagcga taatggaata
     8521 ttgcgggctg gtcgaagtgt gcatcaaata ggcttcattg aagacatgac gattgagctt
     8581 ttttgtttcc gattcactca ctaaaatttg cgaagcctgg ctgaggccgg cgagtaactt
     8641 atgtgtcgac tgagtcgaaa aaataaccga ctccttggct cttggccggt ccttgccgat
     8701 cgcgtgcata tctttataga aatcatggaa tgcggcatgg ggcaaccaag cctcgtcgaa
     8761 atgaagcgtg tcgatttttc catccaacaa ttgcttcaac gtctcaacat tatagataac
     8821 tccgtcgtaa gtgctttgag tcagtgtcag aattcgcggc ttccgctttt tatttttgat
     8881 aaatggatta ttgtcgattt ttttctggat actttccggc gaaaactcct ctttcggaat
     8941 cgggcctata attccatagt gatttcgggt gggagttaaa aacaccggca ccgcccccgt
     9001 catcataatg gcatgaaggt tggacttgtg gcagttacgg tccaccacca cgatgtcgtc
     9061 cggcccaacc gtcgagtgcc acacaatctt attagaagtc gaggtcccgt tggttacgaa
     9121 aaataaatga tcggcgttaa aaatccgggc cgcattcctt tcggaggcgg ccacgggccc
     9181 ggtatgatcg agcaattgtc ccaattcatc cacggcattg cacacatcgg cgcgaagcat
     9241 gttttcacca aagaactgat gaaacatttg acccaccggg gacttcagga aagccactcc
     9301 ccccgaatgt ccaggacaat gccaagaata agaaccattt tgcacataat taagcaaggc
     9361 tctgaaaaac ggcggcgcga tggaatccag gtaagagcgg gcttcacgga tgatgtgtct
     9421 cgctacaaac tccggagtgt cttcatgcat atgaataaaa ccgcgcagtt cccttagaat
     9481 agtattcggg atatggcgag aggtgcgagt ctcaccataa aggtaaatcg gaatctcggc
     9541 atttttaaaa cggatctcct cgatgaaagc gtgaaggact ttcaaggctt cttcggtctc
     9601 ttcagggcta ccgccgccaa actcttcgtc atcaatggag agcaagaaag cggaagctcg
     9661 gctctgctgt tgagcaaagg ctgtgaggtc cccgtagctg gtcactccca aaatctccat
     9721 accttcattt tcgatggcgg cggcgagggc gcgaatgccc aacccactgg cattttctga
     9781 gcggaaatct tcatcaataa tcacaattgg aaaacgaaat ttcaccggcg actcctgagg
     9841 tttttgcaga gaatttatgt atgaagtaca ttctctgatt aggcaaataa aaccattgac
     9901 tcatgaatta ttatcaataa atatgcgcaa acaatgcata aatatccagc gacaaatcca
     9961 ctctcaactt cacctcacgg gtttctcgga acctctggtt ccatttcctg ttccatagtg
    10021 ggcgctcgag gctactcagg acttgagacg gcccgacttt tactgcaaca tccctacgcc
    10081 cagctcactc attgttttgc cacgtctgag tttaagcttt ccaattttat ttccaattcc
    10141 ggcgccgtgc aggtgcagtg tttgcccgat tcggaaatta tgaaccatct gacggatgtg
    10201 gtttttctcg ccactccggc gaacgtgtcc ctgagtttag ccccaaaagt tctgagcgcc
    10261 ggaaaaaagg tcattgatct cagcggagcc tttcgcctta aaaagaatga ctaccaaaaa
    10321 tggtatggct ttgcgcacac ggaacaagag gcccttactg aagcttgcta tggtttagca
    10381 ccctgggtgg gaccagcttc ggaggaaagg cttattgcga atcccggctg ttatgccacg
    10441 gcgatcgctc tggctttgat tccacttttg aaaaatcatt tgattgcgga aaactccatt
    10501 gtcgtggatg cgaaatcagg cacttccggc ggcggcaaaa aggcggttga gaatttgctt
    10561 ttcactgaag tggacggcga atgccttcct tacaaagtgg gctgtcatca gcattatcca
    10621 gaaattatcg aagcccttga ggctttttcc ggcaaaaaaa ttgaagccca tctgaccact
    10681 tcgctcctgc caatccgccg tgggatcatc gccggggttt acgcccaact gcatgaaggc
    10741 atcggtctgc aagaggtcgc cgcagccttc gcccaagctt acggggatta tccgctggcc
    10801 agctttggcc ctctgaaaac agagcccact ttgctttcgc tgaaaaaagt ggtggggacg
    10861 gcaaaaacac acattagctt cgaagtggtc gggcaaagat tgtatgtttt ctcgtgtatc
    10921 gacaacttgc tgaaaggtgc cgccagccag gccattgaaa atttcaaccg catttcagat
    10981 ttgccaattt ctaccggtct cagccatttg gaggcattga catgacacca ggacctgggg
    11041 ttcatcgcac ccctttaccg aagggttttc ttgctagtgg agttaactgc ggagttcgcc
    11101 gttacagacc cgatattgga atcctgattt ctgaagtgcc ctccgtggcg gccggggttt
    11161 ttactctgaa tgaatgcaag gccgctccgg ttcattacag tcagcatctg ttgccagctg
    11221 acaatattaa agccatcctc accaattctg gccaggccaa tgccgccacc ggcgctgagg
    11281 gtgtggaaaa aaatcttatg atgatctcag ccacagctaa aagcttgaac tgtgatatga
    11341 accaagtcct ggtcgccagc accggtgtga tcggagttca gttagatacc gacaaaattc
    11401 tgccttcgat tcctgaactt gtgtcccgcg ctaacgacac ggcggaaagc tttgccaccg
    11461 ctatcctcac caccgattta gtgcctaaaa cagtgaccgc tgtggtcgaa ctttccacgg
    11521 gttcggtccg catcactggt atcagtaaag gttctggtat gattcatcca aatatggcca
    11581 ccatgcttgg ctatatcctg acggatgcca aaatcactaa agaacatgcg gcggagctta
    11641 ttaaaaagac tgcggatatc agttttaata tgatcagtgt ggatggggac tcctccacca
    11701 atgactgttg ctttttgatg gccaacggag ccaccggcgt tgcaatcaag acagccgaag
    11761 ataaagaaaa atttgaagcc atcgttcaag atattggagt cttcttggct aagtccatcg
    11821 ccgctgacgg cgaaggcgcc actaaactta ttgaggttga aatcaagggc tccgacaatc
    11881 ttgccttggc caagaaagca gcccgcggcg tgaccctttc tcctttaatt aaaactgcgg
    11941 ttcacggcga agatcccaat tggggacgta ttctggcaag attgggagct gaacaggttc
    12001 ccgtccagca gctagagaaa atgactcttc gcctgcaagg cgtgcttatt tttgaaaaag
    12061 gccagcctgt ggcctttgct agagacgaag tcaaagctct tcttaagcaa gtccaagtta
    12121 aaatcgaaat tgatttaaaa tccggccccc actcggccac cgcttggggc tgtgatcttt
    12181 ctcgtaaata cgtcgatatc aatacggagt attcatgaag aaaaaaatcc tcatcaaact
    12241 cggcggttct tccctggaga accccgcaac cgtgcgcgaa ctggtcgccc tcaccagcgg
    12301 ctaccgtaaa aaaaattatg atatcatcct ggtccatggc ggaggacctg ccataaatca
    12361 agaactcacc cgccgtggaa tcgagtggaa attcatcaat ggccaacgtc aaaccactcc
    12421 gcaaatgatg gacgttatcg aggaagtttt ggcaaaacaa attaactcga tgctggtctc
    12481 tgttttatta gcggctgaaa tccccgccgt aggaatatcg ggaattcaag acaagacttt
    12541 gctctgcagc caagccaata gcgaactcat gcaagtggga aaagtggagt ccgtaaatac
    12601 ggcggcgctt gaaaaaatcc tccagctcga gctcggtccc actcccgtga ttgcccccct
    12661 tggtgctggc cttcacggtg aaaaatacaa tatcaatgcg gactgggcag cagctaaaat
    12721 cgccattgct ttgcaggcgg aaaagctgat tttcttaact gaccaaaatg gaatcctaga
    12781 ccaagacaag caactggttc atcgagtgac ccctcagttg atcaataaaa tgattgaggc
    12841 tgaggctatc tctgggggca tgtacaccaa agtcatgacc atgatgacgg ctttaagcgc
    12901 cggcatccca caggttcgag tcctcaacgc caacgtcgca agccagctcc tgaataacga
    12961 cgaaattggc acgctcttgt ccaatgatct cccccctgcc agaaaggaag tgacggaatg
    13021 gaatcaaaag ctgaactaaa aaatgattct cactccagag ttctggtttt acgccaactg
    13081 attcgcgaag gaaccgccag tactcaagaa gatttgagca aagctctcaa attaaaaaga
    13141 ttcaacgtta ctcaaagtac catctctaga gatcttcgtc gcattggggc catcaaaacc
    13201 accaacatgg aaggtcagac gatatacaag ttgccagaaa ctcatcagac attgccacca
    13261 gggggaaatc attccttagc cgggcacttg atcgatattc agtccaacga aagtatgatt
    13321 attttgcaca cgtcccccgg ctccgccagc ctcgtggcac gtcacctcga taatatgagg
    13381 tccgctcttg gaattctggg cacagttgcg ggtgacgata ccattttcat agcccccgct
    13441 tctataaaaa agatttcctc tctcattcgt agaatcaaag aggaaatcta atctattctt
    13501 tatttcacta agatattacg gaactgccaa ggatcctgag tgtcaatgtc ctctgggaaa
    13561 agtcctgggc gatcttgaag cggatgccaa tcagtatagt aaccttttac tggacccaag
    13621 taaggcaact ggaattctaa gcaacgctta aaatccatct catcagcttc caccactccc
    13681 gcttttggat tttccagcgc ccatgccatt cccgcaataa ccgctgaagt gacctgtaaa
    13741 cccgtggcgt tttgataagg agccaatagg cgagtttctt caatcgaaag ctgtgaaccg
    13801 taccaataag catttttctc gtggccataa agaaggactc cgagttcatc caagccatca
    13861 acaatctcat tttcatcgag aatatgctgt tcaggctggc gagctcctgc gcgaccgaac
    13921 atttcatcca aagacaaaac ggcatcattg cagggatgat aagcatagtg acaagtcggt
    13981 cggaattcag cgttagcacc ttgaccaacc gtgaagtaat ccgcaatgga aatcgcctcg
    14041 ttatgggtga ctaaacgacc atattgaggt cccggagttg gacaccaagt tctcacccga
    14101 gtattggctc ctggttgttg taagtaaatc gccgcttggc agccgaattc atggcggcgg
    14161 ccggtctcgg gcattgattt ttcatgggtt ccccagccca gttctgctgg ctgcattcct
    14221 tcacaaataa atccatctac agaccaagta ttcacgaaat tgttcattgg cttaggattc
    14281 ttggcccgtt gggtatcgcg ttcagcgata tgaatgccct ttactcccac tttttgcatg
    14341 agctttgccc attgctcgcg ggttttcggc atctcataat ccattttcaa gtcattggcc
    14401 aaattcacca aagcttgttt tacaaaccaa gaaaccattc ctgggttggc gccacagcaa
    14461 gacaccgcag tgatgccgcc tggattggtg gctttttctt tcaaaagacc ttcacggagg
    14521 gcataattcg aacgcccgga agcgcctttg gaatcatcaa aataaaaacc tacccagggt
    14581 tcaaccacag tgtcgatgta atgagcgccg atatcacggc aaagattcat gacgtccaaa
    14641 gaagacacat ctaccgacaa gttgacacag aagcctgtgc cagaaccatt tttcaacaaa
    14701 ggaagcaaaa tttccttata attggccgcc gtcaaaccgc tgtgaataaa gcggattccg
    14761 cgttcatcca aaagcttacg atcacgatca ctcggatcaa tcaccaccaa acggctttta
    14821 tcaaatttaa aatggcgctc gatcaaagga aggcatccac gtccaatgga gccaaaacca
    14881 atcatcacaa tcggaccagt aatttcgcca taattcgcag aaggaggaac aaaagaagta
    14941 cttttagtca tgatataaaa tcacccgtgt tttgcttaag gttattgttc aagaagtgag
    15001 ttcctttacc gaaaatgcgg ctgaagtaca attccttttt tttctagagt aaaatttaaa
    15061 aactccaatc aaataaagga gcaggaacgc caaagactct tgccaaagcg tcggactaag
    15121 acttcctcta aataccgtgg agtttctttt caaaaagcca gtggcaaatg gcgagctggg
    15181 atcgaagttg aaggaattca taggaatctg ggggatttca ccaaagaaag cgatgcggct
    15241 ctggcctaca attcggcggc gcgaaagtat ttcggtaaaa ttgcttttca aaattccgtg
    15301 gggcgaaaaa aagttccacg taaataatct ttcatcccta gacaacttat tacttttgtt
    15361 ttggaaaacc ctagtgtacg agtaggtatg taagggggac agaaaatgtt aacaacgaga
    15421 atcagtaaac agatcgacaa ggccatttta tgcttaatag gactatttat attttttacg
    15481 gctcaatcca gcttttcagc caatggatct aatgaaatca aaatctggaa agattctcgt
    15541 tacaatttac ccagaaaaag cggtgtcgtt ctttttaaac ttaaaaaaaa taagcagctc
    15601 aacgacctcc aatcctcttt tctaattcct ttagcggaag atattggctt gaccgatact
    15661 cgtctcttag gctcctcatc catttatgaa ttgaattttc aaatacccct cgccactatg
    15721 agctccgcag ctcatcatgc acatcatgta gaagaggctt ttgccaataa attaatggcc
    15781 agtggactgg tggaattcgc ggagccagat tatattttca ctcccgatac cgtcactaac
    15841 gaccccaata tcgctgatca atggtatcta caaaaaatct ccgcgcctgc agcctgggac
    15901 gtaaccacag gaagtaataa cacccaagtc ctggtctgtg atacgggaat catgagcagt
    15961 catcctgacc tcaatgagaa cattttaaac caaggacata attttgttga tgatagcggt
    16021 aatacggagc cctcaggaaa tccccatggc accttggtgg caggccttat tggcgcaaaa
    16081 ggcaacaatg gcatcggaat ggccggtctg aattggaaca ttcaaattat tccgggtaaa
    16141 atttcaaacg atccaaaggg agccgctcaa tcctcaacga tggtaaaatg tattcaatgg
    16201 gccatcgatc ataatatcag agtcgttaac ttaagctaca gcggcaccga aagtgatgcc
    16261 atacgggatg cttcaaaaac gctctttgaa aataatggcc tcttaattta caccattggc
    16321 aatgcgggtg ttgaagtcac tcgtccgtac aatccttatg ctttaacggt gggggcttcg
    16381 gacgattatg attttaaacc tccatggtcc aatacaggag aagccttgga tctggttgcc
    16441 cctggggttt ggatctactc caccgatgga aatggcgctt ataagaatgt acaagggacc
    16501 tcctttgccg cgcctctcgt gacgggtact gtcgctcttt taatctcttt aaaccctgag
    16561 cgccctatga aagagctccg acaaatcatt atcgactcag ctaaagatct gtggactcct
    16621 ggcaaagata aagcctttgg ttttggcaga ctggatatca gcaaggccat ggaaatttat
    16681 agaagctccg ctgcagttgc gaacacaaca gtaagggata actaatagtt tcttctcaaa
    16741 ttgagaaaaa ttctcttcag gtcctcttaa tttaatttaa gaagaaagct aaaactttct
    16801 ctttaaaaat atcatcctct catgcaacct tgaatttaca tacatgtgag acagtgcctg
    16861 ccggaactgt aaccagatcg ttcatctctc tccgacaact tataagaaaa aagttagtta
    16921 aaagacttag tctaactttg gccacgaggt cctgatgtag gtatgtacag gggggaacaa
    16981 atgataactc ggtttttcaa aaaatgtgca atagctttaa catttgcagt cttcatactc
    17041 tgtttccaga actgcgccag agaaacttca ttatccagca gctcgaggga agcagcttca
    17101 ccacctgctg aaattattct taataccgac agcaaggacg aggtgaacac ctccgactat
    17161 aatctcacct tttccactaa aattaaaaac aatgatccga tgacttccat cacatgccag
    17221 ctcaataacg agcccagtca ggattgcagc tccggttcta ttgtctttcc ctctcttgcg
    17281 gatggagatc atcaagcaac tatttcgacg gtgactagca ctggagcaat cacccaaatg
    17341 aaaagggtct ttcgcaaaga taccacaggt cctgtgttaa ccgtaacgaa taatatttca
    17401 gcggtaactg ctaagacctc cgcaagtttc aagttttttg caacagatgc cttgagcaga
    17461 gtcagaggct ataagtgtgc tctagatggg aaagccttct cccgttgctc ttctcctctg
    17521 aatcgaagcg gcttagtttc aggaaaaagt tacagcgttt ctatcagagc ttcggataga
    17581 gccgggaact taagtaagat ctattctttc tcttggaccg tgaaaggcgg tgttccgacg
    17641 gctgtgctct cgagcaatct tccatccccg atgacttctt ccacccaaat tactttgacc
    17701 ttcacaggaa ccaatgcagc ttcttttaag tgctctttag atggggctgt ctctacggtt
    17761 tgcacatcgc ctcaaacttt aacggtgtca gctaataaac ttcatcagtt ttccgtgact
    17821 cccatttcag caacttctgt tttaggcgct gcgagttcct actcttggac tgtggatact
    17881 ttacctccca catctccgac tttgttagcc aacatcccca cctccaccac ttctaaatcg
    17941 gcgactttta atttttcttc gactgacaat ctttccggag tcactcacta cacctgttct
    18001 ttaaataatg ctgcgcctat tacttgcact acgccttata atttaaccgt gaacaatgat
    18061 ggagcctaca ctctcgcggt tcgggcagtg gactcagcgg ggaatacttc cgctccgggt
    18121 tctttctcct ggacggtttc tgcgaccatg ccacctcctg ctgacggcaa aacattgttt
    18181 gctagcaact gcgccagttg tcataacggt ccggatactt ttgattctac aaaaacaact
    18241 aaaaccaatc gcacttcggc gcagatctcg gcatctatta actcccttgc tcctatgaat
    18301 accatagcag gacttaaaac ccttaccacg gtgcaaattg atgccattgc cgcttatcta
    18361 aaaaccactc ctccacctcc tgctctggct tgcagcactc cttttgctcc tgaaagaact
    18421 cccctaagac ttctttccaa tttggaatat gactctattg ccgctgatat ttttctttcc
    18481 aaaagaaagc ccagtttaga tactaagttc gatgttcctg ccctaggatc ctccggcttt
    18541 tccaatacca gcatgacgag tgaaacctcc tctcctccca tctccgattt gacagtggag
    18601 aaatactgga ccgcggccgg tgcccttgct aatgaagtta ttgctaataa atctcagagc
    18661 ggaagcgcgt actctgcact ggcttcctgt gctgttggtg tctcctcagt tactgacact
    18721 tgctacaact cgattctccg caatgtcact ttgaaggttt ggcgccgacc cgtctctgaa
    18781 acctctacca ataatgagtt cagccgactg atgccgattc tcaaaagcgg cggaagcttt
    18841 gatgtgggtt ttcgtgactt cctcaaggct ctgctcataa gccccaactt cctcgtagtt
    18901 tcttttactc ctggcacgac cgtgagcacg accagccagg ttttcactct taataattat
    18961 caattggctt ccagactttc cttcttcctc tggcaatccg ctcccgatga caccctctta
    19021 ggccttgcgg ccaatggcac cctctctaac accaccactt tgcaaagcca ggtccttcgc
    19081 atgttgaaag atcctaaagg aaaacgcttt gcctcggtca tgaccaatga atggatcgga
    19141 gccaatcaaa tcctaggctt gggcttaacc acagttgata cggctacttt aaattccatg
    19201 gtcgcggaaa ctcagttcat gtttgaagac attgtgaata ctgatagcag ctttctaaac
    19261 tccatttccg ctaattattc tttcctgaac aaggtcctgg cggattatta caaagttccc
    19321 ttcaacggag ccgtgagcac gcaattctac aaaaccagct tggactcaac tcctcgtcga
    19381 ggagtcctca atcaagccgc cttcttgatt gcgacttcgg gcgctcctaa tcagactcat
    19441 cctgtttccc gcggcaaaat catcgctaga aaaattgcct gctatgatat cgctcctcca
    19501 ccggcaaatc tcgacacttc tttgcctgcc aatattcccg caaactctac accggcggaa
    19561 atcctggcca tccataccca acgtgcccag tgcgcaggct gccacaactc cattgatccc
    19621 cacgggctgc cattagagac ctttgactca cgaggcattt ggcgcacaaa ctatctagat
    19681 attggtggca gatccattga tcaaagcggc aaacttcctt caggcgagac cttctcctcc
    19741 actcaggaat tcatgagcac actgatcaat agctccaatg tgagaagctg tctggtcaga
    19801 aacgttatgg ccgcaggttt agcccgcaag gtggcttctc tgaatgatca gtgcaatgcc
    19861 acccagatca gcgaactcag catgaataca aattctaaac tttctgattt gatcagcaat
    19921 atcgtcataa gccgtcaatt tagaatgcag acaacggaga caccatgagc aattttaaaa
    19981 tatctcgtcg aaaatttatg agcagtacgg ggatgatttt tactatcccc cttctggaga
    20041 gtcttttccc tttgtcggct gaggccgcca atgacccccg acgctatgtg atcttctatt
    20101 ttcctaacgg cacctacaac cgctccgaca aacctatctg ggccactcga gatggagccc
    20161 tcagcagtag caataccagc ttagctttat cgcccttttc agccaattat ggagatatga
    20221 tttcaatcaa ttatttaaaa aatgatgcca atttcggctt ggccattaat gacgatcacg
    20281 cacgaaatgc ggcagcctat ttaacctgcg cctccagcct cagcagctct atgatttcct
    20341 tcgaaaatgt tctggctgat aaattcggaa aggctgctct ggttttaagt ggaggggtca
    20401 ccagtgcgga tctccctccg gacagttata tttcttaccg taatggaaaa ggaaatatgg
    20461 gaatctccaa ccctggcgat ttatatcgaa atctcctcaa ccaagtaatc cctaatgcgg
    20521 gccccactcc aacaccgact cctacggtaa ataatggcaa aagcattttg gatgccacca
    20581 tcgctgactt aaactccttc atgaagacct taggaagatc cgataaagca aaaatggagg
    20641 aattcttaac ctcggtccgc gctctggaac tcaaagtcgc tagcactgcg cccactccag
    20701 ttcctgcccc catgaccgga agcggaacag ggtgtgtgaa gcccactctt gattctaact
    20761 tggattcagc gaattcacga gacgcggccc tctacctgcc taaattttat gccatgaatg
    20821 atatgattaa aattgccttt gcctgcgata tcacacgatc cattagtgtc atggtggata
    20881 ctgaaaccac cactcgcacc tatgccaaac ctccttcatc tcttatctac aacggagctg
    20941 atatcagcgg aggttacact tcgcacattg caatatccca tgccagtggc ctttcaactt
    21001 cgggatataa cttggctgta acccgagacc gcgtcctcat gcagattcca atggatcttg
    21061 tgaataaatt aaaatcagcc aatgatccca gcgggagccg aattctagac aacacaatta
    21121 ttcaagcagg ttttggagtc caagatggga atcatagtgc ctccaacaac caacgccctc
    21181 ttattttagc cggcggccga aacatgttga atctgggaac ttcacgcaat ctgagttctt
    21241 atcaattaaa agatctctat tacaccattt ccaacaaaat aggagcgggc ctgactaact
    21301 ttcaaggaag cagcaccctt atttcccttt gaaatgaccg aaaaaatagt gttcaaggcc
    21361 ccttatgggc cttgaacact gcctctgcgt tttttagaag ggggcagttt gtgccaactg
    21421 atgatagaaa atatgccaga cctgggtcta ttggttttta ttaaaacaag tctttattct
    21481 atcccaatgt tttcattgga cttctggttg tactcaaaca gaagtaaagt cgcgctacga
    21541 gtttcaaatg aataataagc gccttcgcga cttatgatag ccgtatcttc gtctaggaga
    21601 aaatttaaaa cccttctata aaacggagag ccttgaacac tatttattca aaacaaaact
    21661 gtatacgatg tcttggaagc ctatacttct tttggaaaat atcacgtcac tgtctaaata
    21721 tggaacaatg gcacacttag tgaaggtgga atataggtat gcaagcaagt tttgaattaa
    21781 ggaattccat gaaactgaca aattccattt taaaaacatt gtacagcttt tgctttttgt
    21841 ttttagtaac tgtcgccgct aatgcccaat gcccacaaca gttacaaagc gacaccctct
    21901 caacagagtc atttctccgg gacttaagaa agcatataga cactgagaac gaaaatacaa
    21961 ttccttatta tgttttaggc ttagatgagg gtttaacagc attttacaac aaggttaaag
    22021 aagatacaca tcatcaaact ctgtactatg ataccctcta tgaattttct ctttcagacc
    22081 atccgctgaa gagcaaactc ttaaaagaag ttaaacttca aaaaaaatat catagcgttg
    22141 aagagtggat gccagtgttt gaaaagacaa tgcaaaattg ggtggtagct gctccaaata
    22201 gaaaaacaat ttatttcaat ttagatcggt tcgattttaa gaaataccag caattttcct
    22261 ctcagattca agatactata ggcgaaaaaa aggcctctag caaatggaca aatttcacca
    22321 atgcagagat gaaatttatt ttatcggata aaaaaatatt caacaaaata cgttggttcg
    22381 tgaataatcg tgaactcact agctctgaaa tcaaaagaac ttttaaagat gttgctccgc
    22441 aactgttcta aaagtacaaa ataagaaaaa tgtattaaat tttctgatcc atggaagatc
    22501 taaggactta aatattcaac caagtagagc aggcgatatt cgtcagttga aattggtcat
    22561 tggccgcgtg tccaggtccc ttccaaattc ttaggaaatt gccttttatc agtttcgcct
    22621 tatcagtttc aatcaccgat ttaagatcca tatcgacaaa gttaggtccg ttatatttca
    22681 ccgctacggg agattcttta ttgacgtaag cagccaaccc aaccattgca ctgttattgg
    22741 caggctcgtc atattcaaaa aattgagtga tttcgtcgtg ggaaaaatga tagccgaatc
    22801 gattttcttt gttttcaatc acaacttccc cctgatctgg gaaacctgtc gctccagtgg
    22861 tattaatatg tacagtcacc caacttccgt cagcattgcg gcccgtgcat tcggaaatcg
    22921 cccaaacttg aattgagaaa atgaggtttg ctaacaaaat gagatatttc acaagagtac
    22981 tccttaacct taattcctaa acttaatttc ttaaaaacca aaaaacaaac tcgatgccgc
    23041 ccaaagtcat cttgccggaa acttggcagg cgccattttc aggaagcaaa caagcgcccc
    23101 attcaggctc aaaagtcatt gcacccttaa ctgtttgagc tttgtcaaaa acagtcattt
    23161 catatttatg aaaaaatcca cgggcgtgac tcgaggccgg cgttattccc gtcgacttca
    23221 cgatctggac cttacgatag acgtcctcag ggcaatggcc gacgggctcc atcaccaaag
    23281 tttcgtcaga caacttcacg ttcagctttc ccggttgaaa ggcgcagccc tcggaaccaa
    23341 aacttaaact cgcattgacg tagttcgcag ggttgaccgg attccaccaa atgctcgggc
    23401 tgtagttgcc gatattcaaa agctcaatca aatattcaga atgcacaaaa ttcgtatcat
    23461 cgacgccgct gatccaaaga tactcatgca gaaccaggcg caatcttgat ttggtttcgg
    23521 tcgagcgacg taaatccatc caacgagatc ggttgacttt gataagacgt tttgtcggat
    23581 agtaattcac cgcatcccgt tcataaccgt ccaacagggt gtgatcctca gagaccactt
    23641 ctgtcgccgt gattgccact cttaagttca cggtatcaaa agcgggttta ttgttattga
    23701 aaagcagttc caatctttgc aagacatccc gtcctgtcaa aataaattca gaagccaccg
    23761 catcaccagc attgccatga tcccagcccg caaccccccc agagcttgcc aatatcacga
    23821 agactccaag ataaagagaa aggatcctca tggctttttc tcctgcgata aagggaaaag
    23881 ctgaatattc aaacgataga ccgcatcccg agatccttct tccaaatagg tagacaaacg
    23941 acggcgaaaa tcttgaatca taaccttcgc ttctgccaag cgcgactttg aaaccgccat
    24001 ggtgattcca ctcacatcgc gcacttcgac gggatccacc tccaaggaat gaagcacttt
    24061 caaaatatat tgtcgatgcc cctcacgtaa agccgccgaa ggaatctgag acggtgtcgc
    24121 catgtttttt cccgtcaatt gccaaatatt ttttttcttt acaataaggc caagtttttc
    24181 taagcgattt aaacactctg agacaatccc gaagggaata ttcaagcgtt ctgaaatcgc
    24241 ccgttcttgg cccttaaaat ccttgatctc taacaaggcc agaatcgcaa aatgctgcca
    24301 ggaagagatg gcttcggcac tctccatcgc cagttcagta tagctgggaa cggctgccgc
    24361 ctcatccgag gcaaaggtgc tcacgatcaa cgcctgagcc tccacagggt tcctgatatt
    24421 caatccctcc acaattttac gggcagtttt aagcgtgaga ggacgcttgc cctttaaaat
    24481 agccgagagc gtggaagaat ccattcctaa agagcgagcg aaagcgcgga gagagtagga
    24541 agagtttttc ctgcatctct ctgcaaaagt gcgtttcatt tgctcaatca aggtagactc
    24601 cttcatcgcc aatcacccct actttaccgt taaggccgca tttggagctt ccccgaatac
    24661 gctcggcata tacaaggcct cagcatgggt cggaggaagc ttgaacgttc ctggattgtt
    24721 taactggaac tgatatttca cggtcgtgat accagagtct aaatattcaa aatagaattt
    24781 ataacccgag taacttttct gaccagcgct ataatcacca taggcatcac ttaagatgtt
    24841 gcttcccgca ggaatgggat cttgcaacgc cacgttgttg acattgccgg tggagttgat
    24901 cttcaaagtg acttcaacga tatctcccgc ctggaaacca gactcacggg ttacattgtg
    24961 aagttccttt tcgatggaga gtccctggcc tcgcgccgcg atcaagggca cagcgctcaa
    25021 tccttgaatt cccacccaag gttgcccttg acctgaatgc gtcacctgaa cagtcgcttt
    25081 agtactgatc catggtgccg cgatttccgc tgtggtcgat tgactccact ccactgtctt
    25141 actgacttgt tgctctgaga tagccatcac ggtggaaccg ctcacgacct cggcctcata
    25201 agctcgaccg aaggttttca aactttgggc gacgaacatc attgtcgaag tattgtacca
    25261 tgctttggta tttacattca ccaagccgat cgcaagactt cgagccacat ttttatctct
    25321 acgaagaagg gcatggccta aaagtaagtt cgcggtttcc atcgtttcat cagaataaaa
    25381 ataactataa cttggactgc cgctgagttg agccatattg cctgtgtaga caagacgcga
    25441 aggacctaag agctgcgcgt aaatcggaga agtcgctgcg gttgtgggtg tcgcaaagac
    25501 ttgcaggagc cagaaattca ctaaatcact attggaccat ttctccactg gcgggccaaa
    25561 ggccaaggga ttttgctgta attccgccgc caagctggtg ctcacagatt tcgccaatgc
    25621 ttgaagcttg ggatccgcga aagcaaaagc actgcgtccc atgacggttt gtgctgtcat
    25681 ccagtccatc ggtgttttgc cgacatagtc gggatttact gattttgaca ggacctgaga
    25741 aacagctcct tttaattttc ctataagagc cgcaggcatt aacttcaaag cccaaggctc
    25801 ctgttggagg gcattcaaga tcgaggccgt caaccaaacg ctgccacgtt gagcttgagg
    25861 gtagtactta acaaagccgt tggtatccgt agaaccgagc agagaaacga gcacacgttt
    25921 taattcctca ggcttgcttt ctgtactgtt caaaagtgcc ttgtagaatt gcgactcgat
    25981 aaagaaatcg ataaacgtat cgcgactcat acgttgctga atctgcttca ccgctccgtt
    26041 gaccagacta ttggaaactg aaactcggat ttcacccttg cccggaagag cgttcagctc
    26101 tttggttagc tcctgagaga aggcgccact ttccatctgc gcaaggtatg aggtatggat
    26161 cgccaaggga accgatggaa gaatgacttg ggcatcgggc tccatcgcat ccacaacttt
    26221 tccgttctcg tcgtaaatac gaatcacata ctcaatgcga tgggcttgtt cgtccactgt
    26281 gaactcaccc agatcaaccg tttctgacgc tgaagacttg acgtcggatt gctgggtcag
    26341 cgtcttggtg ccaatcactt taccattgct atctctcatc accacttcga cttcggtctt
    26401 aaatttagaa tctttaccac tattattttg caccgtcact ttaatcgggt agctatctcc
    26461 actatgggct actgaaggga tgttcgaata aacttgagtg tccttttcgg tgagataacc
    26521 ggcctcgccc gtgccaaact tttgcgctga atcggtcgcg atggcaaaaa tcttgaatcg
    26581 agtcagactg tcattggttt ggaaagaaaa cttcgcaacg ccattcacca ccggaacact
    26641 cggttggaac gccaccagcg cattaaagag tttgcgtttg aagtcggatt tggcacttcc
    26701 atcgccgcct tcatcgccgc ccttacgact gtcttcgtca gggaggtcgg cattatccgc
    26761 tgtcacggtc tcgacccgtt ccagaggaga aatggtctct acgttgtgcc cgcgcagttg
    26821 cataagcgca tcgagaactt tataagtatt attggctttg agttctaaaa tcttttcttc
    26881 gatggccacc aaagtcaccg tgccacccgc gagtttgcca tcttcaggct gaacgttcac
    26941 ttggacgtcg acggtttccc ctggctcata tttttcttta ttggttttta ctgtcacctt
    27001 ggatttgaaa tggctccagt tgactctcat attggcgtaa cccagacgat aggtcgggcg
    27061 tcccagatcc gtctccccta ttttcaaagc cgccgtctgc gcacgaccgg taatggcgta
    27121 aatggagaca aacgcattcg gagccaaatc tgcatggaca ggaatttcga caaagccttt
    27181 ctcacaggcg ttggagacca aagtggcttc catcacttcg ctgcgctcga tcgttaccaa
    27241 agcacggcaa tttttaaaag gagccgggaa actgacaacg gcacgctctc cgtctttata
    27301 ggttttctta ttcaaagcca agggcagttg tttattccct ggatcatcgc cgaagttgta
    27361 ataatcgttt gctccataaa cccggccatc actatcgact ttgaaaagcg tattggcggc
    27421 ttgcttcgta gtgagcgagg tcacttggaa agcatagttt cctgccttca aagttccgac
    27481 ggggcaattc accacttgtt tttgtactac acaggtttca tgccaacgaa cgggcttgag
    27541 ctcccgttcc agagtgtttt taatcaagcc tccatagagt tcttcaccca tcacttttgt
    27601 ttcaacacgg atcaccttaa tttccagatt ttttagatct gtgatgttct tttgattcac
    27661 atccatgacg gcggcacgaa ggcgggcttc gcttcgattc cctgaaagga gactggttcc
    27721 cacgtactca tgagagttaa agatatcttt ggcctgagag agtgtttgat attctcccat
    27781 ttgatcctga tagcggactc gaaccactaa tttttgcgga cgacctaaag tttttaagac
    27841 ttcagcgacg ctgcgtccgt cagcggcatt ttcaagcgca atatcttttg taagagatcc
    27901 atccgcagcg gtgctcaaac cttgaatcgt cgctggacgg ctgccgcgtg gcaagacctc
    27961 ggtgctttcg gcactttcat cggcactcaa cttcacagcg ccactaccga aattaaaatc
    28021 actgagcatt ttattttgga tggaagtagg ttcaaaataa taagacatat ccgccgcgag
    28081 attttttgct cccacaccat tggcatagcg gatcaccgcg ctgacgggaa tggacgaggg
    28141 ctgaactaaa gcctgagtcg gaaatgaaat caagcccgac atcaaaggca ccttaaactc
    28201 agccacttcg atatctccgg aagctacgtt gatggattca ccatcacgag ttccagtcaa
    28261 tttgatataa taacgtccca attttacgga ggagtcatta ggcacattcc atacaatatc
    28321 tgcaacgccg tttttccagc tcaaaggaag ttcatagtaa gtatcttcat catcagcact
    28381 tgagatccgg gccacggtag gcagtttatt gaccgcaacc tcggtaaagc ccttatcaag
    28441 gggaagcttg gcaatcaact cgatcggaac ttgctggcca ggtttgacta gattgacccc
    28501 gatcacactg tggaaatgag gttggttttc agaaatatca ctataaaacc actcaatacc
    28561 gggagccgaa agcgcatagg tattcgggct cgtccaagaa gaatggatta aggccaagtc
    28621 atcggcctgt cgggccacaa tataaaaggc atctttggca aaataaggag aataagaata
    28681 ctgaggagct ttacaatctt tagcccactc ttgatttggg aaccttacta agcctgaacc
    28741 atccgtcttc aaagttttta cttgctgtcc cagacagttg taaatttcca cgtccgcatt
    28801 cgcgaccggt tgagcctctg acaaacgcgt cacccaagcc agagtattgg tttgaccttt
    28861 tttaaggtga acagccaaat ctgtcacttg cgctaaaaca tatttaggat tgtaatactg
    28921 ttcatcttcg gagttcgaac gaccggattc gaaagtaggg ctagaaattt caatagcgta
    28981 aaaaccactc gacggcgctt tgcctgcttc cccaaaaggg aattgcaaaa agttcggtcg
    29041 gtttttcttt ccggttaagc tttgccctat cacggtgttt ttaacaccca tagcatccag
    29101 gggactggta taagtcggag tatttctgta ttctccgcgg ttttcatagc cccggattac
    29161 attgatcatt gaaggaacgt cttgaatggg ttcccaaatt tttgcattcg tgccggtttt
    29221 gcgaatagat aacttttgat ttaaattcac aactccgact ggcagataca aattcggcac
    29281 atttttttca aagaaggaaa tggtttgcgg aacgtggatc acttcactca tggtgccgat
    29341 acgaatatgg aattccgcaa tcgcattggc aagaatacga ccttcaatat ctttaaggcc
    29401 ttttggcaaa acaacctttg cttgggtctc tggctcaata tttactttga aaataatgga
    29461 atccacaacg gtatcactgt atttcgccag gaatggaaag taatcagaaa gagtgtttaa
    29521 aaaactttcc caaaggctga cttcttggtt cagttctgga taacttctta ctaatttttt
    29581 atcggctgat ttataaggaa tatactcgat gtaagattct ttaatatccg tccactttgc
    29641 acgaccattt aaggctacgg agataggaga gcgcggcaaa caagtggcgt tttttgcact
    29701 gggattggca cactgaactt gggcttgaaa ctgactgcgt acagaaaatt ctttagtgaa
    29761 cttcttctct gaatgaatgc tgccattggc ggcactgctt tgattctgga tcgtaaggtt
    29821 gacttgcgcc ccgggaatca agttttgctg aattgttgcc aacacccaat tctgatcttt
    29881 gtattccaca ttgaaataac gattgctagc aaaatgagag aagatttttt cggattggtc
    29941 ctcaggcacc gccaccaaag gcattttctc actcggggca ttcgcactta aataacttaa
    30001 aaaaccactc tgatcggcaa agaacttgct tcgatcgaca gggccgtcaa aaaggatcag
    30061 aatgacgggt tcatgctctc gtaaagtgtc ttgaaaacca tgagcggcat aaacttcctc
    30121 tacattgggt ccctggacga tcacggaata attaatggtt cccgccaccc agactttatt
    30181 atctactgaa gtcaaatctt cggtttgcac aatattacat tgggtgccac cgaccaaacg
    30241 tgcggaagag tattcgtctt tagctttaaa attataggtc caaagggtgt cattgtccgc
    30301 ccagctagga aaaccctcaa cgatcggcgt acaagaaacc gtgaaaggtc ccttttgact
    30361 gttcttgctt ttattcatgg agttggggaa tttaattctc aattgtgaga aggattcgtc
    30421 cactttccct atcggttcgg gcacagcccc gttgaagtct tgcaccggcc ccgtcgcttt
    30481 tgcggtttcc gacaatccga ttccacaaaa aaggaaaatc gcactgagtc tgattgcaaa
    30541 tctgattgcg aacctcattc cgaaccttaa atttaaaatt ccgcgcctca taatttcctc
    30601 atccttccag ctaaataaat tttgaatcct cggcgttgta acagggactt caagtcctag
    30661 caagaaccca aagttgtttt gcccgcaaag tgcggcaaaa atttcacacg cggcccataa
    30721 tatgcggaat cactagctaa ttttttaatt tacgttatct atttcccatg cataaaaaca
    30781 taaaaaccac ttttttggct gcactttgcg gggttctcac cctcagtctt atctcttgga
    30841 ttgccactcc cagcgccgat aaaatcatac gcctgtcact gggttctgac cgcgttctgc
    30901 tggcggccaa tggggacctc ttacaaaatc tcagaactga ttttaaaaag cgccgtctcg
    30961 cctggcatcc gctacagcgc tttcccgaaa gtctgcaacg ggcggtggtc tccgccgagg
    31021 atcaaagatt cttttctcac tggggggttg atccactgag cctggggcga gctctcttag
    31081 cgaatattca aggcaaacgc ctccaaggag ccagcacgat taccatgcaa ttgagcgact
    31141 tgattcagga aaatgtattg cttcacaatc aagtcattaa aaaaggttcg gtttttcata
    31201 agttcgcgca aatcacgcgc gcttttttca tagaactcaa atggagtaaa aaggaaattt
    31261 tagaagccta tctaaacctc atccatttgc gcggagaatt tcaaggggtt ccagcattga
    31321 cctatgccta tttgaaaaaa gatcctcttg ccttagaagc cgccgaatca ttggtcatcg
    31381 cttcgatgat ttcttctccg aatcaaagtt tgatctccct gaaggccaaa gcttgtctgc
    31441 tcaacagaag gcaggaccat gcaacgcccc cctcttgtga aaaagtgaat gccgctgccg
    31501 aatttttttt caacaaaagt ccggctatgc ccgtcagtcc taattacgct ccccatcttg
    31561 cacggcgctt gtttaatgag ttcgctgagg acgcgattct cacttccacc atcaatagtg
    31621 accttcagaa aaaagtctct gccattttag agaaaaatgt ttatcgtctt aaagactctc
    31681 atgtccatga taccacggcc attgtgatcg acaatcggag tggtaaagtt ttagcctacg
    31741 tcggcacagt gtcctcttct gaaaatccac atgtggatgg cattctctct tatcggcagg
    31801 cgggatcgag cctcaaacca tttatttatg ccaaagcctt ggaaactaaa acactgacgg
    31861 cggcttctat tttactagat gatcccactg caatttcttg gaacggagag gtttatcgtc
    31921 ctgcaaacta tgataaacac ttttacgggc ccgtcgccgt tcgtgaggcc ttaggctcct
    31981 cactgaacgt gcctgcggtg aagacagtca ccatcatcgg tttgcatgag acctaccagg
    32041 tgctccagtc gatgcagctt tctcatttga aagaacctga tttttatggc gtgtcgatgg
    32101 ctctcggagc cgttgaggtt cgtcttgatg aactcgcgaa tgcttaccgc atgctcgcca
    32161 acggaggagc ctggtctccg ctgcggttca cgcaaaaaga catagccaaa gatttcaaac
    32221 ctaaatctgt attttcgcca gaggccgctt atattatcgg ttctattcta tccgaccccg
    32281 atgcgagggc gatcgggttt ggttgggaga ctccactgga aacacctttc tggaccgccg
    32341 tcaaaacggg aaccagcaag gattatcgtg acaattggtg cctcggattt agtgaaaaat
    32401 atacggtggg agtttgggcg ggtaacttta acgccgaagc catgaaaaaa gtcagcggcg
    32461 tttccggcgt gggtccttca tggtatgaaa tcatgaccac tcttcatagc caagagcgca
    32521 gttctccacc ccccatgcca tccccggtga tcgccaaaga cattcgccac caatgggcga
    32581 gccatgttca taaagagtat tttattaagg gaacagaacc cactcaggaa gtgattgaac
    32641 ccgctctgga taaacgaatt cagtttgttt ttcccgccga tggttccgtc ctcgtcaaag
    32701 acccgcatct ggaccaacag tacgtggccc tttttgtgcg ctttaaagga tcagtaccca
    32761 agaagagtca gttgctctgg gatggaaaac ccttgggtga agccgtgagt ccgtttaaga
    32821 tcccagatcc cgcgagcggc gatcacgagc ttgcggtggc ttcaaaagac ggaaaaattt
    32881 taggtaaaat ccgcttcacc attcacggtg ctgagtaaaa tctactttct tttttgctga
    32941 tgacctttac taagacattt acaatctaca aattcctgtt cacaataaat ctaagacgct
    33001 gtatttttaa taacttgatt ggagcaatat gcagagttct aataaggctg ggatagcact
    33061 tgtggaaatc cttatagcct ttggtctgct ggcaattctg ggccttggca tttcctccat
    33121 gatccaactg ggattcatgg gacaaaaggg catagaaaaa gattatgcca tcaataatat
    33181 cagcgaacaa attcgaggta ttttaggtga tccgaaagcc tgcaaaaata cgtttggagg
    33241 acaactcgtc ggagcatctg ctcaacctat tacccagatc cttgatggct ccactccgcc
    33301 cgtttccgtt tatagcagtg gaagttccac tgtatatgag tctagaaata ttaaaatctc
    33361 agccatgaac ctaaaaacgg actctactat aggcaacgtt ccc
//
LOCUS       DATBTE010000138         1895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_17986, whole genome shotgun sequence.
ACCESSION   DATBTE010000138 DATBTE010000000
VERSION     DATBTE010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17986"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..1820)
                     /locus_tag="VIG33_05185"
     CDS             complement(255..1820)
                     /locus_tag="VIG33_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8270260.1"
                     /translation="MTLQLQNLSEKKKALAECDTSRFISLGNSFKEFVSEIKIKRFRH
                     IADLTLTFEHPVTIISGTNTIGKTSILLLLACSFERFLKIDSTSPSPDLREHSWKDVL
                     QFTSHENVDNDYSYELKWRSVNQKRTGEAKRLSSSRSWSGLGKKSTDRSRINAKIRDR
                     EVRLIDLERILPGRSFSSALYRKANAASRVRLNKEVEKAFSYVFDIEQIELFEVGVHI
                     NKSCFLISRPEESYSSFNAASGEEAVIYLLKDIIESPKNSLILIDEIEAGFHPSVQRK
                     LADIVQYISWRDKKQFVITTHSPTLISAFPSKSRRFIEKSFLGYRVIPDISHQAVRSK
                     MDSVGDPLIKLYCEDDLASFLIKKILIDITRDYPHFHKLVNIIKSGPIDQVKNDYNRH
                     KKNFSQFRNKIGYCAIFDGDYKEDPSYSKYFENSSENTLFIYPYDAPEKFLIRAYLME
                     NDNQELRAALEHEDHHTLFNKMIGCGLATSELDAQSCCYSAFSNTPEYQKHKSDMSSF
                     LISVVKKFSELQD"
BASE COUNT          566 a          377 c          347 g          605 t
ORIGIN      
        1 ggtagccctg cccgccacgg gcgcggctgt gacgctgatt ttgagctgct tgcctttggc
       61 cttgccgatg gcatcctgtt tcgcgctagg ctggccgaga acattaagca ctagcgtttc
      121 gccatcccag gcgcagaaag gattaatcgc caagcctttg tttttcataa attcatacta
      181 ttgcaagcct gaacaaaata ttgcgtaacg tttgggttag ttacggaatg ttacattaaa
      241 cgtcgtgaag cacatcaatc ctgtaactct gaaaactttt taacaactga tattagaaag
      301 ctagacatat ctgacttatg cttttggtac tcaggagtgt tgctaaaagc agaataacag
      361 caagattgag catctaactc ggatgtagct aacccacacc cgatcatttt attaaataat
      421 gtatggtgat cctcgtgctc aagcgctgct cgcagttcct ggttatcgtt ttccataaga
      481 taagctcgaa taagaaactt ttctggagca tcgtatggat atatgaaaag agtattttca
      541 gaactatttt caaaatattt tgaataactt ggatcctctt tgtaatcacc atcaaaaatc
      601 gcacaatacc caatcttgtt cctgaattgt gaaaaatttt tcttgtgacg gttgtaatca
      661 tttttcactt ggtctatagg accagattta ataatattaa ccagtttatg aaagtggggg
      721 taatctcttg ttatgtctat aagaatcttt ttaattagaa aagacgcaag atcatcttca
      781 caataaagct taattaatgg gtctcctact gaatccatct ttgatcgcac cgcttgatga
      841 gaaatatctg gaatcacacg ataacccaaa aacgattttt ctataaatct gcgtgactta
      901 cttggaaatg cggaaattag tgttggtgag tgagttgtaa tgacaaattg ttttttgtca
      961 cgccatgaaa tgtattgaac tatatcagcc aattttcgtt gaacggatgg gtgaaaacca
     1021 gcctcaatct cgtcgatcaa gatcaatgag ttttttgggc tctcaataat atcttttaaa
     1081 agatatataa cagcttcttc accagaagct gcattgaatg aagaatatga ctcctctggc
     1141 cgagatatta aaaagcagga tttattaata tgcacaccaa cttcaaaaag ttcgatctgt
     1201 tcaatatcaa atacgtaaga aaacgccttc tcgacttcct tgtttaatct cacccggctt
     1261 gcagcatttg cctttctata aagagcactt gaaaaactgc ggccaggtaa aatcctttct
     1321 agatcgatta gacgaacctc tctgtctcta atttttgcat ttattctaga tctatctgta
     1381 ctcttcttac ccagtccgct ccaagatcta ctcgatgata aacgcttggc ttctcctgtc
     1441 cgtttctggt tcactgaacg ccatttaagt tcgtacgaat aatcgttatc aacgttttcg
     1501 tgtgaagtga attgaagcac atctttccaa gaatgttctc gcaaatctgg tgagggtgaa
     1561 gttgaatcaa ttttcaaaaa acgttcaaaa ctacaggcta aaagaagcaa tatacttgtt
     1621 tttcctattg tgttagttcc agaaattatt gtcacaggat gctcaaacgt aagagttagg
     1681 tcagctatgt gacgaaaacg cttgatttta atttcactca cgaattcttt aaagctatta
     1741 ccgaggctaa taaaccttga agtatcgcac tcggctaacg cctttttctt ttcacttaaa
     1801 ttttggagct gcaaagtcat atctttattt cttggcaaaa caattattat tcaggatcgt
     1861 cgaatgacaa cgctataggg atgcattaac aaaga
//
LOCUS       DATBTE010000139         4974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1384824, whole genome shotgun sequence.
ACCESSION   DATBTE010000139 DATBTE010000000
VERSION     DATBTE010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384824"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VIG33_05190"
     CDS             complement(<1..405)
                     /locus_tag="VIG33_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEY8270261.1"
                     /translation="MLRYLSSSLFLSIIALIGIGLVQVYSSSFIFAIESYGDGLFFFK
                     KQLVFALIAFVALIGAIHLPFKYVEKFGWLIWLASAALVVATFIPGVGARVGGAIRWI
                     RLPWGVRFEPAELLKVSFSLLFASLLCRQNNFL"
     gene            complement(509..922)
                     /locus_tag="VIG33_05195"
     CDS             complement(509..922)
                     /locus_tag="VIG33_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270262.1"
                     /translation="MIKSLSVVLSLVLSLSGMAGVLFLTGCASSAYKARQEQREKVAS
                     SAGLYCEWINGDKHSDIDVELNLQMGKRCDSVKPFTLTPYKNTSDENGIVYCCALTGA
                     EPKAPSSSGIKKASGPAAQKAATATPAADEVVEDK"
     gene            complement(953..2341)
                     /gene="murD"
                     /locus_tag="VIG33_05200"
     CDS             complement(953..2341)
                     /gene="murD"
                     /locus_tag="VIG33_05200"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY8270263.1"
                     /translation="MFREVSELKDKRILVVGLGKTGVSLAHFLCKYEAQVTVTDHKSK
                     PELSAQLEQCEGLPIRFDLGGHSPKTFLQQDLVILSPGVPSSLKIFDYARSQGIKITG
                     EFEFSAGFIKEPIVGITGTNGKTTVARLVEAFLNKSGIKAWVGGASEKPLVEYLRSDE
                     KAQVVVAEVSSFMLEHSESFNPMNVVFTNLAENHLDRYRSMEEYVNAKRRIFKNTNQA
                     TTSILNADDNAVVELARDPQVQRGRIFYFSRKPALEPQIMNIGGAVNIGDEIRVRTGP
                     DIEYYTIKNMKMKGKHNIENLMAAILAAREHGAKHDAIQTVINGFLNLPHRIEYVRKV
                     GGVQFFNDSKATNVHAALRALDTFDENVILIAGGKDTNLNYEPLRNIVKRKVKTLILV
                     GEAKERINRELGDYSETFLIGTFEEAVLIAYQKSRIGDIVLLSPSCSSFDMFDSFEER
                     GDYFKEIIKKFN"
     gene            complement(2348..3424)
                     /gene="mraY"
                     /locus_tag="VIG33_05205"
     CDS             complement(2348..3424)
                     /gene="mraY"
                     /locus_tag="VIG33_05205"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY8270264.1"
                     /translation="MLYQWLYSFADQFSPLNVFRYITVRTFISFFTSFFMAWMWGPYF
                     INRLKEKHFGQSIRDDGPQTHKKKAGTPTMGGGLILLSALIPCLLWVDLLSPLVLAVV
                     AVTLGFGLIGYMDDYLKVSKKNSKGLAGKIRLLGEFLIAGITVLILIHYFELSTVVTV
                     PFAKNLNFDLGYFYVLFAALVITGTANAVNLTDGLDGLAIVPVIISASTLGLFSYVVG
                     HYSIATYLQIPHVIGAGELAPVAAAMVAAGLGFLWFNTYPAQVFMGDVGSLSIGGFLG
                     IMAVITRNELLMLLLGGVFVVEALSVITQVISFKLTGKRVFKMAPIHHHYELKGLTET
                     KIIVRFWIISILLAVLSLATLKLR"
     gene            3554..4972
                     /locus_tag="VIG33_05210"
     CDS             3554..4972
                     /locus_tag="VIG33_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270265.1"
                     /translation="MKRLSLHQCLILSFIIYIIVVTFNTGFIATDEYWDGITRYLPAQ
                     KATVNTLIRLDDVKSPLQILPMHAAAQLAYKLGLETPFSQYHFVIIFWGLIGFALSAS
                     AAILLFRGKSESLAKVAVLMLGFHFVAPGLLTRPMFESLAAPWMALACAFAVLYDEKR
                     HWQNLLAGVFCASMSFTLRQQTGFCALVFILLPLLHKQWKHFFLASFSGLTLFILSGV
                     PDIFIRGGFHYSLRALAEYNFKHGAEYANQPWHFFFPLIFIGTLMPWLIAKYPAGFLG
                     DYIKRYRSLYIILALFLILHSMFANKFERFLISMIPVLIFIMVPFVNYFVENWAQRKY
                     RLISMLAVNLVFWFPASFFPAQKNITDLARYMDEHPEYRALQSIDLSIEWIPDAFMKQ
                     VNYTVFQLSTSQLQTQAIPVNCDTLVALNEKAFASNREFLLFHYKFLARFDVNMIESI
                     AYKFNAENNVRRSPLMIFSCKK"
BASE COUNT         1398 a         1220 c         1018 g         1338 t
ORIGIN      
        1 caagaagttg ttttgacggc aaagaagact ggcaaaaaga agactaaaag aaaccttcaa
       61 cagttcagcc ggttcaaaac ggactcccca gggaagccgg atccaacgga tggctccacc
      121 gacacgcgca cccactccgg gaatgaatgt ggccaccact aaggccgccg aagccaacca
      181 tatcagccaa ccaaattttt ccacatattt aaaaggcaga tgaatcgctc cgatcaaagc
      241 cacaaaagcg atcaaggcga agaccaattg ttttttaaaa aagaaaagtc catcaccata
      301 agattcgatg gcaaagataa agctcgaact ataaacttga actaaaccaa ttccaatcaa
      361 ggcaataata gacaaaaaaa gactgctgga aagatagcga agcatttgaa actcctctgt
      421 tttattctaa acagaggagg gtctcttcgt cttgtagtca gattaaaact ggactatgaa
      481 aaaactatga gagaagtctg ggatcttttt atttatcttc gacaacttca tcagcagcgg
      541 gagtcgcggt tgcagctttt tgtgccgcag gaccactggc ttttttaatg ccgctagaag
      601 atggagcttt aggctcagca ccagtcagcg cacagcaata aactattcca ttttcatctg
      661 aagtattttt ataaggagtc aatgtgaagg gcttgacgga atcacaacgc tttcccattt
      721 gcagattgag ttcgacgtca atatcggaat gtttgtcgcc gttgatccac tcacaataaa
      781 gtcctgcaga agaggcgact ttttctctct gttcttgacg ggccttataa gcgctggaag
      841 cacagccagt taaaaataga acgcccgcca ttccacttaa agacaacact aaagacaata
      901 ctacagataa cgatttgatc acagctccct ccatggcgct tgtaatgggt gattaattga
      961 actttttaat gatctcttta aaataatcac cgcgttcttc aaagctatca aacatatcaa
     1021 agctcgagca actcggtgag agcaagacga tatcgccaat tctagacttt tgataagcaa
     1081 tcaggaccgc ttcctcaaag gtaccaatca agaaggtttc gctataatca cccagctcac
     1141 gattgatgcg ctccttggct tccccaacca ggattaaagt cttaactttg cgtttcacga
     1201 tattgcgcaa aggttcatag ttcagattgg tatctttgcc tccggcgatc aagatcacgt
     1261 tctcatcaaa agtatccaag gcgcgaagcg ccgcatgaac gttcgtagcc ttagagtcgt
     1321 tgaaaaattg aactccgccc actttacgca cgtactcgat acgatgcgga aggtttaaga
     1381 atccattaat gacagtctga atggcgtcat gtttagctcc gtgctcgcga gcggccaaga
     1441 tggctgccat caaattctcg atgttgtgct tgcccttcat cttcatattt ttgatggtgt
     1501 agtattcaat atccggcccc gtgcgcacgc gaatctcatc cccgatattt acagcgccac
     1561 cgatattcat gatttgaggc tctagagcgg gcttacgaga gaagtagaaa atacgtccgc
     1621 gctgaacctg aggatcccgg gcaagttcca ccaccgcatt gtcatccgca tttagaatac
     1681 tcgtggtggc ctggttcgta tttttgaaaa tacggcgttt ggcatttaca tattcttcca
     1741 tcgaacgata acgatccaaa tgattttccg caagattcgt gaagaccaca ttcatgggat
     1801 tgaagctctc actatgctcc aacataaaac tagagacttc cgccaccacg acttgagctt
     1861 tctcatcaga acgcaggtac tccaccagag gtttttcgct ggcgccaccc acccaggctt
     1921 tgataccgga cttattcaaa aaagcttcca ctaaacgagc cactgtggtt ttaccgtttg
     1981 ttcccgtaat gccgacgatg ggctccttaa tgaagcccgc actgaactcg aactcgcctg
     2041 taatcttgat tccttggctc cgggcatagt cgaaaatctt gagactggaa ggaactccgg
     2101 ggctcaagat caccaagtcc tgttgcagga aagtcttcgg actgtgcccg cccagatcga
     2161 aacgaatggg aagtccctcg cactgttcca gttgtgctga taactcaggc tttgatttat
     2221 gatccgtcac cgtgacttgc gcttcatatt tgcacagaaa gtgcgcgagg ctaacgcctg
     2281 tttttcctaa accgacaact aaaattcttt tatcctttaa ctcactcact tctctgaaca
     2341 tgatcactca cctcaatttt aaagtcgcaa gacttagtac cgctaataaa atagatataa
     2401 tccaaaaacg aacaataatc ttggtctcgg tgagaccttt tagctcatag tgatgatgga
     2461 tcggtgccat cttgaaaacc cttttgccgg tgagcttgaa ggaaatcacc tgcgtgatca
     2521 ctgaaagcgc ctccaccaca aagactccgc ccaacaagag catcaacagc tcattgcgag
     2581 tgataacggc cataatcccc agaaagcccc ctatactgag acttcccacg tcacccataa
     2641 aaacttgggc gggataagta ttaaaccata agaacccgag tccggccgcc accatggctg
     2701 cagccacagg agctaactcc cccgctccaa tcacatgggg aatctgcaaa taagtcgcga
     2761 tcgaatagtg gcccacaaca taggagaaca agcctaaggt gctcgcggaa atgatcacag
     2821 gaacaatggc caagccatca agaccatccg tcaaattgac cgcatttgcg gttccggtga
     2881 taaccagagc ggcaaacaaa acataaaaat agcccagatc aaaattcaaa tttttcgcaa
     2941 aaggcaccgt gaccacagtg gaaagttcaa agtagtgaat caaaattaaa accgtgatcc
     3001 cagcaatgag gaactcgcct aaaagacgaa ttttaccggc caggcctttt gaattcttct
     3061 tgctgacttt caaatagtcg tccatatagc cgatcaaccc aaatccgaga gtcaccgcca
     3121 ccaccgccaa aaccagcgga ctcagcaaat ccacccataa aagacagggg atgagagcgc
     3181 ttaagagaat taacccccca cccatcgtgg gagttccggc ttttttcttg tgggtttgcg
     3241 gaccatcatc gcgaatgctt tgaccgaaat gcttttcttt caaacgattg ataaaataag
     3301 gaccccacat ccaagccata aagaaagagg taaaaaagga gataaaagtg cgaactgtaa
     3361 tataacgaaa aacattcaaa ggagaaaatt gatccgcaaa cgagtagagc cactgataaa
     3421 gcatctttta aaaatccttt tttatagatt tttctaattt tgtgcctaag ctttgtttgt
     3481 gtaacacaaa ggccaaacaa aaagcaagaa aatgtttgca agatattgaa atcacttcta
     3541 ttttacttag attatgaaaa gactttcttt gcaccaatgc ctgattctct cctttattat
     3601 ttatattatt gtcgtgactt ttaatacggg cttcattgcc accgacgaat attgggatgg
     3661 catcacccgc tatcttccag ctcaaaaagc caccgtgaac actttgatta ggcttgatga
     3721 tgtaaaatca ccgctgcaga tactgccgat gcatgcagct gcacaattgg cttacaagct
     3781 gggccttgaa acccctttta gccaatatca ctttgtcatt attttttggg gtttgattgg
     3841 ttttgctctg agcgcctccg ctgccatttt gctctttcgt ggaaaaagcg aaagcctggc
     3901 taaagtggcg gttcttatgc tggggtttca ttttgtggct cctggtttgc tgactcggcc
     3961 gatgtttgaa tccctcgccg ccccttggat ggcgctggcc tgtgcctttg cggttctgta
     4021 cgatgaaaaa agacattggc agaacctctt ggcgggagtg ttctgcgcct ccatgtcctt
     4081 cacgctccgc caacaaacgg gattttgcgc attggtcttt atcctgcttc ccctcttgca
     4141 taagcaatgg aagcattttt ttctggccag tttcagcggg ctgaccctct tcatcttgag
     4201 tggggttcct gatattttta ttcgtggtgg cttccattac tccttgcggg ccctggctga
     4261 atacaatttt aaacacggtg ctgaatacgc aaatcagccc tggcatttct tttttcctct
     4321 catttttatt ggcacgctga tgccttggtt aattgcaaaa tatcctgcgg gtttcctcgg
     4381 ggattatata aagcggtatc gctcgcttta tataattcta gccctgttct tgatcttaca
     4441 ctcaatgttt gccaataaat ttgaacgctt tttgatctcg atgattccag tcctgatctt
     4501 tatcatggtg ccctttgtaa attatttcgt ggaaaactgg gcacagcgca aatatcgatt
     4561 gatttcgatg ctggcggtca atctggtttt ttggttcccc gcttcctttt ttccggcaca
     4621 gaaaaatatc acagacctgg ctcgctacat ggacgaacat ccggaatatc gcgctctgca
     4681 aagcattgac ctcagcatcg aatggattcc tgacgccttt atgaaacaag tgaactacac
     4741 tgttttccag ctctcgacat cacaattgca gactcaagcc attcctgtga actgtgacac
     4801 tcttgttgcg ttgaatgaaa aagccttcgc ctccaacaga gaatttttgc tctttcatta
     4861 taagtttctg gcccgctttg atgtgaatat gattgaatcc atcgcttaca aattcaatgc
     4921 cgaaaacaat gtccgccgct ctcccctgat gatttttagt tgcaaaaaat aatt
//
LOCUS       DATBTE010000140         4037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2272515, whole genome shotgun sequence.
ACCESSION   DATBTE010000140 DATBTE010000000
VERSION     DATBTE010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2272515"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..528
                     /locus_tag="VIG33_05215"
     CDS             247..528
                     /locus_tag="VIG33_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270266.1"
                     /translation="MCFLFVSIVMVSSISRATTIICKNEKSVRTLRNDKTSQGCSAVY
                     TKQGVDQVVGSSMNDSACDGIIERIRKTLEGSVWKCRNVKEAVVSNLSE"
     gene            604..1437
                     /locus_tag="VIG33_05220"
     CDS             604..1437
                     /locus_tag="VIG33_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEY8270267.1"
                     /translation="MSQELKVAICQMTSVDDLKKNLQQMEALVGEVQENSGVRLFCFP
                     ENCLYMRIKEGEAIPPFTVQDSVFEDLAVIARYRKAYFHLGSVPLQMGDFLYNSSIII
                     SDEGKIETSYQKIHLFDIHLAGHEPIRESDVFRHGVGAGVFTIDGWKIAQTICYDLRF
                     AELYSQYAREHVDAILVPAAFLVKTGEAHWEVLLRARAIESQCYVIASAQAGRHESSH
                     GGGTRETYGHSSIVDPWGEMVVHLSGTKKQTSVYSLSKPKIEQVRRQIPMAQHRRLPV
                     K"
     gene            1531..1800
                     /locus_tag="VIG33_05225"
     CDS             1531..1800
                     /locus_tag="VIG33_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270268.1"
                     /translation="MTQKFKIGVRSVSIFIGLIGVLSSAAYGVEVIAGSLFDESKEAE
                     IAQKARRHAYPGGRDEGDLAVQSQLMAPTRKVAPQVENAESSADD"
     gene            1841..3571
                     /locus_tag="VIG33_05230"
     CDS             1841..3571
                     /locus_tag="VIG33_05230"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEY8270269.1"
                     /translation="MAINDQLPEGVTRETMDVDVLIVGGGAAGLSCALHLQKQIEKYN
                     EEIASGKKQGPPIPEQMIVVLEKASEIGAHSFSGAVLNTKALEELIPDFKEQGCPLEA
                     QVTKDAVYYLGEESAFKLPITPPPFHNIGHHLISLSKFNRWLAEKCEAKGINIFPGFA
                     AVEALYDGDKIVGVRTGDKGRDKNGAPKANFEPGLILNSKCVIFAEGTRGSLFKKVSN
                     KLNLRAGKNEEVFEEGVKEVIQMPAGTVEPGQVIHTMGFPLNKSIGGTFIYTLPGDKI
                     IVGLVAYLDTHDPLLDPHRELQKLKTHPFMQKMLKGGKVIAYGGKTLPAGGWYSMPKL
                     HGDGWMVCGDSASMVDVQKLKGIHLAMKSGMAAAETALDGIIKNDFSSTVTQAFEQKI
                     YESYVKKELYRVRNFHQTLSKGLFESLPLIAFQEMTGGRGLKDNMSAHKDSKATHKVI
                     EVWGSEGMEAEENKLPKPDGQLFYDKLSSVYLTGTMHDENSPNHLFIGNGDICRTVCE
                     PEYKSPCNHFCPASVYEMLPSQKHQGQLELQVNYTNCIHCKTCDIKCPFENIEWTAPE
                     GGGGPQYRET"
     gene            3612..>4037
                     /locus_tag="VIG33_05235"
     CDS             3612..>4037
                     /locus_tag="VIG33_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEY8270270.1"
                     /translation="MPEFFASTPKGLVEALEKELQELNLKVLERTAGGVFFEANWEGA
                     YKANLHSRVASRILKPVLEFTAYQPEELYKQIQNHDFTKYIKPNQTICIDATIKESKM
                     HDQRYVAMKVKDAVVDQFRGKFGVRPDVDNNNPDLRIHVR"
BASE COUNT         1172 a          765 c          961 g         1139 t
ORIGIN      
        1 taaatgccat gaaagtacaa gttcaaggcg taaaagtaca aactcatttt gataaaaaag
       61 atggtttgtt gaaaaaagag ggcttcattg attattctaa tgttaagctt ctggatgctc
      121 ctgctaaaga aaagaaaact acaaaaaaag caacaaagtc taaaactgct tagttgtaga
      181 gtgttaaggc aagaaaagtt tccttttttc gcattctcac ataaaggctc tgaagttaga
      241 gcctttatgt gtttcttatt tgtttctatt gtcatggtta gttccatttc tagggcaacc
      301 actattattt gtaaaaatga gaaatctgtt cgaacgttaa gaaatgataa aacatctcaa
      361 ggttgcagtg ctgtttatac caaacagggt gtcgatcagg tcgtgggttc ttccatgaac
      421 gacagtgctt gtgatggaat cattgaaaga attcgtaaaa ctcttgaagg cagcgtttgg
      481 aaatgtcgta acgttaaaga agcagttgtt tctaatcttt cagagtaatt ctaagaaaat
      541 gcatttcatt cctatagaga attccctaaa ctaatgcaga accagcagta ttaggagaat
      601 cagatgtcgc aagaactaaa agtggctata tgtcagatga cttcagttga tgatcttaaa
      661 aagaatcttc agcaaatgga ggctctggtc ggtgaggtcc aggaaaactc tggagttcgt
      721 cttttctgct ttccagaaaa ctgtctctat atgcgaataa aagagggcga agcgattcct
      781 ccttttactg ttcaggattc ggtgtttgag gatcttgcgg ttatagctcg ttacagaaag
      841 gcgtactttc atctaggatc agtgccactt cagatgggag attttcttta caattcttcg
      901 ataattattt ctgatgaagg aaaaatagag actagctatc agaaaattca tttgtttgat
      961 atccaccttg cagggcacga gcctattcgt gaatctgatg tcttccgtca tggtgttggg
     1021 gcgggggttt ttacgattga tggttggaaa attgcacaaa caatttgcta tgatttgcgc
     1081 tttgctgaac tctattctca atatgcccgc gaacatgtag atgcaatttt agttcctgcc
     1141 gctttcttag taaaaacagg cgaagctcac tgggaggtct tgttgcgtgc acgtgcgatt
     1201 gagagtcagt gttatgtgat cgcaagtgca caggcggggc gtcatgaaag ttctcatggg
     1261 ggtggaactc gtgagactta tggccattct tcgatagttg atccgtgggg agaaatggtt
     1321 gtccatttga gtggtacaaa aaagcagacc tctgtctatt ccctttccaa gcctaagatt
     1381 gagcaagttc gcagacaaat tcctatggcc cagcatcgtc ggcttccagt aaaatagaaa
     1441 tcacttcgca ggtgacttta ttctcaatat gctttcaaat cacgacaagg caggaagcct
     1501 gaaactggac tagctgtaag gaggatcttt atgactcaga aatttaaaat tggagttcga
     1561 tccgtgagca tcttcatcgg gcttattggt gttttatctt cggcggctta tggcgtcgaa
     1621 gttattgccg gctctttatt tgatgaatcc aaagaggctg aaattgcaca aaaagcgcgc
     1681 cgtcatgctt atcctggcgg acgagatgag ggcgaccttg cagttcaatc ccagttgatg
     1741 gctccgacac gtaaggtggc tcctcaagtt gaaaacgcag agagctctgc cgacgactaa
     1801 ttatcaaatt attttagaac taattttttg gagatgaaat atggcgatca atgatcaact
     1861 ccctgaaggt gtgacccgtg aaacaatgga cgtcgatgtt ttgattgtgg gcggaggagc
     1921 tgcgggcctt tcttgtgctt tgcatttgca aaaacaaatt gagaaataca atgaggagat
     1981 agcctccggt aaaaaacagg gtccgcccat tccagaacaa atgattgtgg ttttggaaaa
     2041 agcttcagaa attggggctc acagtttctc tggcgcggtt cttaatacca aagcacttga
     2101 agagcttatt ccggacttta aagagcaagg ctgccccttg gaggctcaag taaccaagga
     2161 tgctgtttac tatttgggcg aggagtctgc cttcaaactt ccgatcactc ctcctccatt
     2221 ccacaatatc ggccatcacc ttatttcttt gtcgaaattc aatcgctggc tggccgagaa
     2281 gtgtgaagct aaaggtatca atatttttcc aggttttgct gctgttgagg ctttgtatga
     2341 tggggacaaa atcgtaggag ttcgaactgg ggataagggg cgcgataaga atggcgcccc
     2401 aaaagcgaat tttgaaccag gccttatttt aaattctaaa tgtgtgatct ttgctgaagg
     2461 gacgaggggt tctttattca aaaaagtttc aaataaactc aaccttcgcg ctgggaaaaa
     2521 tgaagaagtt tttgaagaag gagtaaaaga agtcattcaa atgccagctg gcacagtaga
     2581 gcccggtcaa gtaatccaca ccatgggctt tccgcttaat aaatccattg gtggaacttt
     2641 tatttatact cttccaggag ataaaattat cgtgggtctt gtggcttatt tggataccca
     2701 tgatcctttg ctcgatccac accgagagtt gcaaaaattg aagactcatc cattcatgca
     2761 aaagatgctt aagggtggaa aggtcattgc ttatggtggt aaaactctgc cagctggggg
     2821 ctggtattca atgccaaagc ttcatgggga tggctggatg gtttgtggag actctgccag
     2881 tatggtggac gtgcaaaagc tcaagggtat tcacttggcg atgaagtcag ggatggccgc
     2941 agctgaaaca gctttggatg ggattattaa gaacgatttc tcaagtacag tgacacaggc
     3001 ttttgaacag aagatttatg aaagctatgt gaagaaagag ctttatagag ttcgtaactt
     3061 tcatcagacc ctgagcaaag gactttttga gtcgttgccg cttatagctt ttcaagaaat
     3121 gactggcgga cgcgggttga aagacaatat gtcggctcat aaagactcta aggccactca
     3181 taaggtcatt gaagtatggg gttcggaagg gatggaagcg gaagaaaata aactgccgaa
     3241 acccgacggg caacttttct atgataaact ttcaagtgtt tatctaactg gaactatgca
     3301 tgatgagaat tctccaaacc atttattcat tgggaatggt gatatttgca gaaccgtctg
     3361 cgagcctgag tataaatcac catgtaatca tttctgtcca gccagcgtat acgaaatgtt
     3421 accttctcaa aagcaccagg ggcagcttga gctccaagta aactatacta attgtattca
     3481 ttgtaagact tgtgatatca agtgtccgtt cgaaaatatc gaatggacgg cgcccgaggg
     3541 aggcggcggc ccacaataca gagaaacgta attccgtata aagggagcgt ttcttagaca
     3601 ggagcgttcc tatgccagag ttttttgcct ccacacctaa aggcctcgtt gaggccctcg
     3661 aaaaagagct tcaagaatta aatcttaaag tcctcgaaag aaccgccggt ggggtgttct
     3721 ttgaagctaa ctgggaaggg gcttataaag ccaatcttca ttcccgtgta gccagccgta
     3781 ttttaaagcc agttttggaa tttacagctt atcagccgga agaattgtac aagcagattc
     3841 agaatcacga tttcacaaaa tatatcaaac ccaatcaaac tatttgtatc gatgcgacga
     3901 tcaaagaatc taaaatgcat gatcagcgct atgtggcgat gaaggttaag gatgccgtgg
     3961 ttgatcaatt ccgcgggaaa ttcggagttc gcccagacgt ggacaataat aatccagact
     4021 tgcgtattca tgtgcga
//
LOCUS       DATBTE010000141         3245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1657440, whole genome shotgun sequence.
ACCESSION   DATBTE010000141 DATBTE010000000
VERSION     DATBTE010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657440"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..686)
                     /locus_tag="VIG33_05240"
     CDS             complement(12..686)
                     /locus_tag="VIG33_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270271.1"
                     /translation="MTEYVIENLDNLNNLELKKRSDIHLARKIWHVVGVSGIFLAYVL
                     LPEMISSLILAAAVVIFLPIDFLRHKYPALNDWMVHAFKPIMRTHEVHRIAGTTYLLS
                     GVSVIVAFFPRPVVALTLLFLAFADPIASLVGIKYGKDKVFGHKSVQGFMAAFVVCAL
                     ATSIYLTYHNIIFDRVIIVSLLAGLIGALAELVPIGKIDDNFTLPVLSGAGLYILFYL
                     FGLFAA"
     gene            complement(804..1352)
                     /locus_tag="VIG33_05245"
     CDS             complement(804..1352)
                     /locus_tag="VIG33_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270272.1"
                     /translation="MMGKDAKIEWKFLVMTTCLVATMAVPTLASLITGDGANSPQAAM
                     ILSPNEQKLRRPASFPRLGAPKKAVVILDTKRELGNLLSNNLVNYDFSCAKVKATSIQ
                     VVGAFLQLKGKDCNKNSQGPKLTIVNKSNGFTASIFLLNTKEYQTDLIQLQEGENQIS
                     IQYQTPSGQIEERVLNVKATAI"
     gene            1599..2153
                     /locus_tag="VIG33_05250"
     CDS             1599..2153
                     /locus_tag="VIG33_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8270273.1"
                     /translation="MADVWDIFSNYIFFIQKAYNLEIHSFILMSNHFHMLLKTPEANL
                     DRAMNYFLREVSKEIGRKSERTNQIFGGPYHRSLLKSEIYYYNAYKYVYQNPVRAGLC
                     QQVEDYPYSTIRNLLGKQKSIIPISGDNIIGENPFTALKWLNQRLDDSTLDCIRRGLR
                     KYEFKVQQDKNSGKLPNLDVPTLD"
     gene            complement(2183..2587)
                     /locus_tag="VIG33_05255"
     CDS             complement(2183..2587)
                     /locus_tag="VIG33_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY8270274.1"
                     /translation="MAMKMDLDNEAIADINVVPLVDIILVVLIIFMVTAPMFIKPTIS
                     VNLPKAASGDQTAPSKLNIALTADGRINLNGTFVDEATVKQKVTDEVTKNPEIQAIIS
                     ADKDVPHGKVISLLDVVKTAGVKKFAISIDKK"
     gene            complement(2591..>3245)
                     /locus_tag="VIG33_05260"
     CDS             complement(2591..>3245)
                     /locus_tag="VIG33_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY8270275.1"
                     /translation="VKERTSMPVLSEKIFAVAHLADQVVLWILVLLSIISIGMILERG
                     FALRKVSSQSSQVRNRIRMALQSNSVEDVETIAKDIESLEGRAASYALKHMRDSGSKG
                     LEEIFNTFSLMEKPELERFLGFLATVGSNAPYIGLLGTVLGIMKAFNDLAMASEAGQQ
                     TVMAGISVALVATAAGLFVAIPAVISYNYYSKKVRGIMQGLEAIKELCLAYAKKKGV"
BASE COUNT          956 a          725 c          657 g          907 t
ORIGIN      
        1 tggccgccta ctcaggcggc gaaaaggcca aacaagtaaa atagaatgta aagacctgcg
       61 ccgcttaata caggcaaggt gaaattatca tctatttttc caatgggaac aagctctgcc
      121 agagcgccaa taagacccgc gagaagactc acgataatta cgcgatcaaa aatgatgtta
      181 tgataagtca aatagatgct ggtcgctaag gcgcaaacca cgaacgctgc catgaaacct
      241 tggacggatt tatggccgaa aactttgtct ttgccatatt taatccccac caaagaggca
      301 ataggatctg caaaggctag aaataataag gttaaagcta ctacggggcg cggaaagaag
      361 gccacgatca cgctcacccc acttaaaaga taagtggttc ctgcaatcct gtgaacttca
      421 tgagtgcgca taatgggttt aaatgcatgg accatccagt cattcaaagc aggatattta
      481 tgtcgtaaaa aatcaatagg aagaaaaata actacagctg ctgctaagat taacgaagaa
      541 atcatctcag gcaaaagaac ataggcgaga aatataccgg aaactcccac tacgtgccag
      601 atcttacgtg ccaaatgaat gtcagagcgt tttttaagtt ccaaattatt taaattgtcc
      661 aaattttcta ttacgtattc tgtcatcacg gtcgccacca taaaggaagc cacgccatat
      721 gatcaaaccc aaaatcactc aaacttgtat attgcgcgtt aagattctat ttgggaagaa
      781 aaaaaatgtg aaatttctca agattaaatc gcagtagcct tgacatttaa gacacgctct
      841 tcaatttgtc cagatggagt ttgatattga atggaaatct gattctcccc ttcttggagt
      901 tgaatgagat ccgtttgata ttcttttgtg tttaataaaa aaatcgatgc tgtaaaccca
      961 tttgatttgt ttacgattgt cagctttggc ccctgagaat tcttgttaca atccttcccc
     1021 ttgagctgaa ggaaagctcc gaccacttga atagaggtcg ctttcacttt cgcgcaactg
     1081 aaatcatagt taacaaggtt gttgctcaga agatttccca attctctttt agtatccaaa
     1141 attaccactg cttttttagg ggctcctaac cgcggaaagc tggcaggccg acgaagtttt
     1201 tgctcattag gactcaggat catcgccgcc tgcggagaat tcgctccatc gccagttatc
     1261 aaagaagcta gagtaggaac agccatagtt gccaccaagc aagtcgtcat taccaagaat
     1321 ttccactcta tttttgcatc tttgcccatc ataccttgta ttcggcaaaa atttgacggg
     1381 actttaggcg tgtcattttg agacggccgg acaggccgga cagtacgccg tacctttttc
     1441 ggatgcagtt ggtcacgagc gcttgtttat tcttatttga ggtaggctta ggccttgcaa
     1501 tttctcgccc catgcctaga aaaaaattta ttcctagttc agactatccc tactttatta
     1561 ctgcgcgctc gagaaatcga gagtggtatg aattgcctat ggcggatgtg tgggacatct
     1621 tcagcaacta tatcttcttt attcaaaaag cttataacct cgaaattcat agctttatac
     1681 tcatgagcaa tcattttcat atgctcctaa agactccaga ggccaatcta gaccgagcca
     1741 tgaactactt tttaagagaa gtgagtaaag agataggtcg aaaatcagaa agaacaaatc
     1801 aaatattcgg cggcccctac cataggagtt tattgaagtc agaaatatac tattacaatg
     1861 cctataagta tgtttatcaa aatccagtaa gggctggcct ctgccagcaa gttgaagact
     1921 atccctacag tacaattcga aatctcttag gcaaacagaa gtcaattatc cccatcagcg
     1981 gtgataatat tataggagaa aatcctttta ctgctttgaa gtggcttaat caacgactcg
     2041 acgactctac tcttgattgt attcggaggg gccttaggaa gtacgagttc aaagttcagc
     2101 aggataaaaa cagcggcaaa ttacctaatc tggatgtacc tacgctcgac tagccgcatc
     2161 cgaaaaaggt acggcgtacc ctctattttt tgtcgatgga aatggcgaat ttttttacgc
     2221 cggcggtttt taccacgtcg agcagactta ttacttttcc gtgggggacg tctttgtcgg
     2281 cggaaatgat ggcctgaatc tccggatttt ttgtgacttc gtccgtgact ttttgtttta
     2341 cggttgcttc gtctacgaag gtgccattga ggttgatgcg accatccgct gtcaaagcga
     2401 tgtttaattt actgggagct gtttgatctc cgctcgcggc ttttggtaag ttcacgctga
     2461 ttgttggttt aataaacata ggagccgtca ccatgaagat aatcaacacc acaagaatga
     2521 tatccaccaa cggaaccaca tttatatccg ctatggcttc attgtcgaga tccatcttca
     2581 ttgccataat ttaaacacct tttttcttag cgtaggctaa gcaaagttct ttaatggcct
     2641 caagtccttg cataatgccg cgaacttttt tgctataata attgtaggaa atgaccgctg
     2701 gaatcgctac gaaaagacct gctgcggttg caactaaagc caccgaaata ccagccatca
     2761 ctgtttgttg acctgcctct gaagccatcg ccaaatcgtt aaaggctttc ataattccca
     2821 aaactgttcc caagagacct atataaggag catttgatcc cacagtagcc agaaacccta
     2881 aaaatctttc aagctctggc ttctccataa gagaaaaggt attgaagatt tcctccaaac
     2941 ctttactgcc gctgtccctc atatgtttta aagcgtagct cgcggcgcgt ccctcaaggc
     3001 tttctatatc tttcgcgata gtttcaacat cttccacact attactttgc aaagccatgc
     3061 ggatgcgatt acgaacttga gaggactggc ttgaaacttt gcgaagggcg aagcctctct
     3121 ccaaaatcat gccaatgctt atgatgctta acaaaaccaa gatccacaaa acaacttggt
     3181 cagccaaatg agcgacggca aaaattttct cgctaagtac aggcatggat gtcctttctt
     3241 tgact
//
LOCUS       DATBTE010000142        11401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_18036, whole genome shotgun sequence.
ACCESSION   DATBTE010000142 DATBTE010000000
VERSION     DATBTE010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 11401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11401
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18036"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..501
                     /locus_tag="VIG33_05265"
     CDS             94..501
                     /locus_tag="VIG33_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270276.1"
                     /translation="MKIQFSFMLILACSISNAASALECAVTDSQGITKSYLLNRNIPA
                     DFDGAKPTTEFSEKGINFGTQAYCRGSETQSKPSEEGSLRMSGTLMNSNAACELHLDS
                     TGTGKYILKIRPMSMSGSVKVISFEVKCEGLGG"
     gene            complement(670..1167)
                     /locus_tag="VIG33_05270"
     CDS             complement(670..1167)
                     /locus_tag="VIG33_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270277.1"
                     /translation="MKVGRNTISLLKTSVYIFIKAILGLSILTSVFGAFYWYDNRLFL
                     WEASLIWPQEPFSESKFKIGSTQDRSRMVVDLIRNEKLLGVASNKIQEQLGGETGDYY
                     YNDSNFTYRLTERGSADWILTLIPGDNGKIRQIFIRKSCCSISQKILYWSLENSESLI
                     RKILQ"
     gene            complement(1142..2521)
                     /locus_tag="VIG33_05275"
     CDS             complement(1142..2521)
                     /locus_tag="VIG33_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270278.1"
                     /translation="MMTQIKFLKMVSAAPIILALIVVMSCAHKELVGELINNPPTYKV
                     EFDFSDPEKLRDHLRKIDDFKTQENQQKLIQILIAYNNLKKAQSKNQITLAPHSKTRI
                     VVASFCAASEKAIPERKEIFKWAKGTPKISLIKEVLDLYAKNAESNIQRVQELIWNLE
                     NETYYDEYPDFLKELVKQASPSAPLTLPSRLKSQIVDELTPQEIKDTINLVKGKYYSF
                     NEFKKVIETKRSNFILPESSFVSKLPETDISATTSSRGYESQIITFYNSTNNIGIFKT
                     TSYYLKPIRDDVQPIILASVFPYENEIQKILEESALKLLGYLGSQYPTLNPSEKALVK
                     QNPIEAAITFYDAFLAERNGDRFFPSSGQNGASDSFRHFAWAGLLTRDLGEETARKFL
                     NAHELNSSQPSEEKAMDEFNNERGILAANQFLKRGNFENAELYEKATEEVKSGKLRIL
                     NYESRQKHH"
     gene            complement(2588..3481)
                     /locus_tag="VIG33_05280"
     CDS             complement(2588..3481)
                     /locus_tag="VIG33_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270279.1"
                     /translation="MKTYSTQVPGKWILAGEHSVLRGVPALVFPLRSRVMDLSYVCAS
                     SATSLNLYLHGEHGEELKLLVLEVLDKACEIQKIPRSSLVGDLTLKSSIPVGAGMGAS
                     GALCVAITKWLSFLGHVQESEMFEFARSLENIFHGESSGVDIAISLSGQALHFERSGT
                     RSTLNVQWMPHWYISYSGQRGVTLECVNKVKSFMALNAALGKQLDQQMKKAVEDCEKA
                     LCQKDEKEGLENLAAGITLASQVFQQWGLTEGAPQAHMDLLKQQGAIAVKPTGSGGGG
                     YILSLWATPLPEGLLGKLIPC"
     gene            complement(3485..4459)
                     /locus_tag="VIG33_05285"
     CDS             complement(3485..4459)
                     /locus_tag="VIG33_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEY8270280.1"
                     /translation="MPSVVASAPSNIALIKYMGKVKTQGNLPTNASLSYTLDSLRTTV
                     RLTSLGDGEGADKWAALKGPEFEPLRLSEKGQERFLAHLQFLKAQWGIQQTFLVESAN
                     NFPSDCGLASSASSFAALTLAAGKMFQQIKPHSWGEDVRVLSSLSRQGSGSSCRSLYT
                     PWALWAAEYAEPVALPYKKLLHQVIIVESAKKEISSSEAHKLVALSSLFPGRIERAEK
                     RLKELQHSLQAQNWRQSFEIVWAEFWDMHALFATCAPPFMYMTSGSQRVLEEILQFWK
                     NQGDGPLVTMDAGANVHFLWREDQKELAQAQENYWQSQFRVISSPTKD"
     gene            complement(4463..5338)
                     /locus_tag="VIG33_05290"
     CDS             complement(4463..5338)
                     /locus_tag="VIG33_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270281.1"
                     /translation="MSSLILSVPGKSFLAGEYLALKGGASLIFNSKPRFEFSAKPGSG
                     GVEGIHPDSPAGKLIHDHRDFFAKYNLSFLDPYQGRGGWGASTAQFLMTFVLNEWRAS
                     CELETFKSLSISRLLESYWKYAWNGQGLRPSGADLVAQYKGSLCLFERRTGLVGVYGW
                     TFGDIDFALIPTGQKIATHEHLKTLAEFDEAPLELAMKSIQQALESGDSAEFVGGIRA
                     NAQALADLGFTAPHTQGLCREMLQRPGVLAAKGCGAMGADVMLVVYQKTASDTFKDFV
                     TSKDLTHGLEIQVKE"
     gene            complement(5352..6647)
                     /locus_tag="VIG33_05295"
     CDS             complement(5352..6647)
                     /locus_tag="VIG33_05295"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEY8270282.1"
                     /translation="MARTLSDIFKGFSKLTRRERLEALEETGALEPRDVDFLEKGGLK
                     DTSLGEKFIENVIGYFQVPLGVATNFRINGKDYIIPMAVEETSIVAAASKTAKWIREI
                     GEITTEVLGGDIIGQIQCARVPDYLAFEKSLLTQKKYLIEIANREVAFGLVRRGGGVT
                     DITVRRVPRGDGQDMAVVHVMMNPCDAMGANIINQVCEFLKEPIEQFTGVKVTMCILS
                     NLVDTKITRAVVVLHDIDAELAQKIEEASLFAQQDPYRAATNNKGVLNGIDPILIATG
                     NDWRAVEAGIHAYAARDGQYRSVTKWKREGDKLRGVFEAPLIVGTVGGMTTLHPTAAL
                     CMKMLGTESANELSCVIAAVGLVQNLGALKALTTVGIIEGHMKLHIKNLTLGAGAEER
                     EIPIVQKKLEEILAIRKRISLSNAIEVLKELRASEQRTR"
     gene            complement(6632..7702)
                     /gene="fni"
                     /locus_tag="VIG33_05300"
     CDS             complement(6632..7702)
                     /gene="fni"
                     /locus_tag="VIG33_05300"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEY8270283.1"
                     /translation="MSESSKPFEKKSFEKRKQDHIRIALDPRSQAEGLSGLSQVQLIH
                     EALPNMNFKEVDASTSFFSRGMSKPLSAPFFISSMTAGHEKAFDINSRLAQLSAEKQI
                     LMGVGSQRRELMDSEAQLEWKKIRQQAPKALLLGNVGIAQVIQTPVDCVQALVDSLEA
                     EALFIHLNPLQECLQPEGTPEFRGGYAAIESLVKKLSVPVIVKEVGCGFSKGTLQRLY
                     NSGVFAVDVSGLGGTHWGRVEGYRSQPEELLFQIAQTFENWGLSTVQSLLDAKEIDEK
                     KPVWASGGVRDGLQAVKLLAMGADMVGLAQPWLKAALESSEAVNVLYEKLLLEVKIAM
                     FCTGVQKLEDFRTKRVWKWHEH"
     gene            complement(7728..8075)
                     /gene="rplT"
                     /locus_tag="VIG33_05305"
     CDS             complement(7728..8075)
                     /gene="rplT"
                     /locus_tag="VIG33_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEY8270284.1"
                     /translation="MARVKSGKTNRARHKKVLKRTKGYYSAGSRCYIHAVEKNDHALQ
                     YAYRHRKMKKRNFRALWNQRINAAARLNGTTYSRLIGGLTKAGILVDRKVMADLAIHD
                     AAAFTALCKHALA"
     gene            complement(8110..8301)
                     /gene="rpmI"
                     /locus_tag="VIG33_05310"
     CDS             complement(8110..8301)
                     /gene="rpmI"
                     /locus_tag="VIG33_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEY8270285.1"
                     /translation="MKMRTHSGAKKRLKLLSSGKIKKKSTRLRHLNSHMSSKVKRQLG
                     KTSYVETANLLQVKRLLVF"
     gene            complement(8506..8796)
                     /locus_tag="VIG33_05315"
     CDS             complement(8506..8796)
                     /locus_tag="VIG33_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270286.1"
                     /translation="MILASHLLDISYERVLSYISIVFGISLLYFGFFCPQWFSKKAPK
                     PSFKDDSYFWQVFPYGLFFLVGGSAFLFQPQLSSLLVALGCAAWVKILYKFV"
     gene            complement(8922..9605)
                     /gene="infC"
                     /locus_tag="VIG33_05320"
     CDS             complement(8922..9605)
                     /gene="infC"
                     /locus_tag="VIG33_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEY8270287.1"
                     /translation="MSKFEGRNDFRGGAGFRGKKDSKDSLRVNREIRAPQIRVIDDEG
                     NMLGVMTVPEALRLAEERNLDLLEIAPTATPPTCKIMDYGKWKYENKKKAAAARKKQV
                     IVSIKEVQMRPRTDQHDFETKMNHARRFLLDGDKVKVNLRFMGREMAHQEIGMNIMKR
                     CMEFVKDLATIETNPKMEGKQMFILIAPDPLKIRDYLKAHPNKSKQDTKELAELKDEE
                     EEEEDEVAE"
     gene            complement(9624..>11401)
                     /gene="thrS"
                     /locus_tag="VIG33_05325"
     CDS             complement(9624..>11401)
                     /gene="thrS"
                     /locus_tag="VIG33_05325"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEY8270288.1"
                     /translation="KVALITTKSPEAAEVIRHSCAHIMAQAVQDIWPEVKVTIGPVIE
                     NGFYYDFDSPFHFTEEHFAQIEKKMSEIVAKDLPIARQNWPIAKAVETFKGLKERFKV
                     ELIEGLAEKGETEVGIYFNGDSWFDLCRGPHIQSTGQIKAFKLLSVAGAYWRGDEKNP
                     MLQRIYATAFGDKKELEQYLHNIEEAKKRDHRKLGKELGLFHFHEWAPGSPFFTGKGA
                     TVYTQLQTYMRELYFEFGYQEVITPQIFDSNLFITSGHMANYKDNIFFTRVDERDFAT
                     KPMNCPSHCLLFKAEPHSYKQLPLRMADFGRLHRFEKSGAMHGLTRVRTFCQDDAHIF
                     CMMDQMQTEIASFMQLLNRVYTQLGMSNYKIYLSTRPENRMGGEEVWDKAEGALTTAL
                     NSLNLPFTINPGDGAFYGPKLDIMFVDALNRPWQLGTLQVDFNLPEAFQLEYIGVDNR
                     EHRPVMLHRAILGSLERFIGVYLEHTAGHLPPWLAPVQVMILNITDRVNLFCEELQQM
                     FKDSQVRVEFDNRNEQLKFKIREAQLQKVPYMVIVGDSEATSRTVSLRLRDGSEHKGI
                     AVDDLMKLVTEDINERKLQSSLMKI"
BASE COUNT         3153 a         2762 c         2339 g         3147 t
ORIGIN      
        1 atttttgtct ttaaatcgaa ataaacaaat ttttgtgatg gcacacctct cgcataatcg
       61 cataatgaac tctccaaaga aaagaggtac ggagtgaaga tccaatttag ctttatgtta
      121 attttagctt gttctatttc taatgctgca agcgcccttg agtgtgctgt aaccgattcc
      181 caagggatta caaagtccta tcttttgaat cgtaatattc ctgctgattt tgacggcgca
      241 aaacctacta cggaattctc tgagaaaggt ataaattttg gaacgcaagc atattgcagg
      301 ggaagcgaga cccaaagcaa accaagcgag gagggttctc taagaatgtc aggcacttta
      361 atgaacagca atgccgcttg tgagcttcat ctcgatagca ctggaactgg aaaatacatt
      421 ttaaaaattc gaccgatgtc tatgagtggc tctgtaaaag tcatttcctt tgaagtgaaa
      481 tgcgagggct taggcggata aaatcatttt tcctaggagc ggcccaaggc cgcagcattc
      541 ctgattttta tatagagatt ttacattacg acacttcggt gctgcgtgat ggccccggtc
      601 tgacggtgaa agcgtggcaa tcaaaagaaa attatatgat tatcaaggac tctttttatt
      661 gttgtgcaat tattgcagta tttttctaat aagagactca gagttttcaa gactccaata
      721 caatattttt tgtgaaatag agcagcaact ttttcggata aaaatttgtc ttattttccc
      781 attatccccc ggtatcaaag ttaatatcca atctgcactt cctctttcgg tcagcctata
      841 agtaaaattt gaatcattgt aataataatc ccctgtttct ccgccaagtt gttcctggat
      901 tttatttgaa gcaaccccaa gaagcttttc atttcggata agatccacaa ccatacgtga
      961 acgatcttgc gttgaaccaa ttttaaattt tgattcagaa aagggttctt gtggccatat
     1021 tagactagct tcccataaaa agagtctatt atcgtaccag taaaaagcac caaaaacaga
     1081 ggtcaatatc gatagaccta atatcgcttt aataaagata tagacagatg tttttaagag
     1141 actaatggtg tttctgccga ctttcataat tcaaaatcct tagttttcca ctttttactt
     1201 cctcagtggc cttttcgtat aactcagcgt tttcgaaatt tcctctcttc aagaactgat
     1261 tcgccgctag aataccacgt tcattattaa actcgtccat tgctttttct tctgagggct
     1321 ggcttgaatt gagctcatgc gcattcagaa attttcgcgc agtttcttct cctaaatctc
     1381 tagtcagaag tcctgcccaa gcaaagtgcc tgaaggagtc agatgctcca ttttgtcctg
     1441 aactaggaaa aaatctatcg ccattccttt ctgccaaaaa agcatcgtaa aacgtgattg
     1501 cagcttcgat tgggttttgc ttaaccaacg ccttttcgct ggggtttaat gtggggtatt
     1561 gcgaaccaag gtagcccagc aattttaaag cagattcttc taatatcttc tgtatttcat
     1621 tttcatacgg aaaaacagaa gctaaaatta tgggctgaac atcgtctcga ataggtttca
     1681 aataatagct tgttgtcttg aagattccga tgttattggt ggaattgtaa aaagtaatta
     1741 tttgcgattc gtaaccacgg ctagatgtcg tggcagaaat atcagtttca ggcaatttcg
     1801 acacgaaact gctttcaggt agtatgaaat tcgacctctt ggtttcaatt acctttttaa
     1861 actcattaaa tgaataatat tttcctttaa caagattaat tgtgtcctta atttcttgcg
     1921 gcgttagttc gtcgacaatc tgagatttta ggcgggaagg caaagttaaa ggagccgatg
     1981 gcgaagcttg tttaaccaat tcttttaaaa aatcaggata ttcatcataa taagtttcgt
     2041 tttcaaggtt ccatatgagc tcttgaactc tttgaatatt gctttcagcg ttcttagcat
     2101 ataggtcgag gacttctttt ataagtgaaa ttttcggagt tcccttggcc catttaaata
     2161 tttcctttcg ttctggtatg gctttttcag aggctgcaca aaaggaagca accacaatac
     2221 gagttttaga atgaggagca agggtgattt gatttttact ctgtgctttt tttaagttgt
     2281 tatacgcaat tagaatttga ataagttttt gttgattctc ttgtgttttg aaatcgtcta
     2341 tttttcttag atgatctcga agtttttctg gatctgaaaa gtcgaattca actttataag
     2401 taggagggtt atttattaat tcaccaacaa gttctttatg tgcgcacgac atgactacga
     2461 taagggccaa aatgattggc gcggcactaa ccattttcaa gaatttaatt tgagtcatca
     2521 tttgattcta catttttgcc atctccgaaa tcaaactaaa acgtgccaat cacccttcga
     2581 aacaaaccta acaaggaatc aatttcccga gcagcccctc aggcagagga gtggcccaaa
     2641 gactgaggat atacccaccg ccgccagagc ctgtgggttt tactgcaata gcaccctgct
     2701 gttttaaaag atccatgtgg gcctgagggg ctccctcggt gaggccccat tgctgaaaga
     2761 cctggctggc gagagtgata ccagcggcaa gattttctag gccttctttt tcatcttttt
     2821 ggcagagcgc cttctcgcaa tcctcaaccg cttttttcat ttgctgatca agctgttttc
     2881 ccagggccgc attcaatgcc atgaacgact tcactttatt cacacactcc agcgtgactc
     2941 cccgctgacc agaataagaa atataccaat gcggcatcca ctgcacattg agagtgctgc
     3001 gagttccact gcgttcaaag tgcaaggctt ggccacttaa tgaaatggcg atatccacgc
     3061 cgctgctttc gccgtgaaaa atattttcca agctgcgagc gaattcaaac atttccgatt
     3121 cttgaacatg gcccaagaaa ctcagccatt tagtaatggc cacgcagagt gctccggaag
     3181 cccccatccc agcacccacc ggaatggaac tcttcaaagt caaatccccc accaaagagc
     3241 tgcgaggaat tttttgaatc tcacaagcct tatcgagaac ctccaacacc aagagtttca
     3301 actcttcccc gtgctcgcca tgcaaataaa gattgagcga ggtggcagaa gaagcacaaa
     3361 catagctcaa atccatcacc cgggaacgca atggaaaaac caaagcggga acgccccgaa
     3421 gaacggagtg ctcgccggct agaatccatt ttccaggcac ctgcgttgag tatgttttca
     3481 tcttttagtc ctttgtcgga gaagaaatca ctctaaactg cgactgccaa taattttctt
     3541 gagcttgagc cagttctttt tgatcttcac gccataagaa atgcacattc gccccagcat
     3601 ccatggtcac cagcggacca tccccttgat ttttccagaa ttgcaatatt tcctcaagca
     3661 ctctttgcga gcccgaagtc atatacataa aaggcggagc acaggttgca aataaagcgt
     3721 gcatatccca aaactctgcc cagacgattt caaaactctg ccgccaattc tgcgcctgca
     3781 aagaatgctg caactctttg agtctttttt ccgcgcgctc aatgcgtccc ggaaacaaag
     3841 agcttagcgc caccaactta tgagcttcgc tcgatgaaat ttcttttttt gcagattcaa
     3901 caatgatcac ttgatgcaaa agttttttat aaggaagagc caccggctcg gcatattcag
     3961 ccgcccacag ggcccaaggc gtgtacaaag aacgacacga ggaacccgaa ccctggcggc
     4021 ttaaactgga aagcacgcgc acatcctccc cccaactgtg aggtttgatt tgctgaaaca
     4081 tttttccggc ggccaaggtt aaagcggcaa agcttgaggc cgaactcgcc aggccacaat
     4141 ccgaaggaaa gttattcgcg gactccacca agaaagtctg ctggatcccc cactgagcct
     4201 ttaaaaactg cagatgcgct aaaaatcgct cctggccttt ttctgacaaa cgcagaggtt
     4261 cgaattcagg acctttaaga gcggcccact tgtcagctcc ttcgccatcg cccagtgaag
     4321 tcaggcgcac cgtcgtccgc agagaatcca aagtatagga aagggacgca ttggtgggaa
     4381 gatttccctg ggttttgact ttccccatgt atttgatcaa agcaatatta gaaggtgcag
     4441 aggcgacaac agatggcatg gactattctt tcacttgaat ctctaaacca tgggtcagat
     4501 ctttagaagt cacaaagtct ttaaaggtat cactagcggt tttttgatac accaccagca
     4561 ttacatccgc acccatggct ccacaaccct tcgccgcaag aacccctggc ctttgcagca
     4621 tttcccgaca cagcccttgg gtatggggag cagtgaagcc cagatccgcc aaagcctgag
     4681 cgttggctcg gatccccccc acaaactcag cagagtctcc gctctccaaa gcttgctgga
     4741 tggatttcat cgccagctcc agcggtgctt catcaaattc agccagagtt tttaaatgtt
     4801 catgagtggc tattttctgc ccagtgggaa tcaaagcaaa atcaatatcc ccgaaagtcc
     4861 acccataaac accaaccagg ccggtgcgtc tttcaaaaag acacagcgaa cccttgtact
     4921 gcgccaccaa atcagctccg ctgggacgga gtccctgccc gttccaggca tatttccaat
     4981 aggactcaag aaggcgcgaa atgctcagtg atttgaaggt ttcaagttca caggaggccc
     5041 gccactcatt caaaacaaag gtcattagaa attgtgcggt cgaggctccc cagcccccac
     5101 gaccttgata aggatctaaa aaagaaagat tatatttcgc aaagaagtcc ctatgatcat
     5161 ggatcagttt gccagcagga gaatcagggt gaattccctc aacccctccc gatccaggct
     5221 tggcactgaa ctcgaagcgc ggcttcgaat taaaaatcag actcgctccg cccttgaggg
     5281 ctaaatattc gcccgcaaga aaactttttc cgggcactga aagaatcaaa gatgacactg
     5341 aaactccttg tttagcgcgt ccgttgctca gaagccctga gttctttaag aacctcaatg
     5401 gcattactca aagaaatcct tttgcgaatc gctagaattt cttccaattt tttttgcaca
     5461 atggggattt cgcgctcttc agcaccggcc cctagtgtta gatttttaat atgcaacttc
     5521 atatgacctt cgatgattcc caccgtggtc aaggctttca aagcgcccag attctgcacc
     5581 agccccaccg ccgcgataac acaagaaagt tcattggcgg attccgttcc caacattttc
     5641 atacagagag cggccgtagg atgcagagtg gtcatccccc ccactgttcc gacaatcagg
     5701 ggagcctcaa agacgcccct caacttatcc ccttcccgtt tccatttggt cacagagcga
     5761 tactggccgt ctcgtgctgc ataggcatga atccctgctt cgaccgcgcg ccaatcattt
     5821 cccgtggcaa tcagaatagg atcaatgccg ttcaaaacgc ctttgttgtt ggtggctgca
     5881 cggtaaggat cttgttgcgc aaataaagaa gcttcttcga ttttttgcgc caactcagca
     5941 tcgatgtcat ggaggactac aacggcgcgg gtgattttag tgtccaccaa gttcgacaaa
     6001 atacacatag tgactttgac acccgtaaat tgctcaatcg gttccttcag gaattcacaa
     6061 acttgattga tgatattagc acccatggca tcgcaggggt tcatcatcac atgaacgacg
     6121 gccatgtcct ggccatcgcc acgaggcaca cggcgaacag tgatgtccgt gacaccacca
     6181 ccacggcgga ctaagccaaa ggccacttcc ctattggcaa tttcaattaa atatttcttt
     6241 tgcgtcaata acgatttttc aaaagccagg taatcaggaa cccgggcgca ttggatttgc
     6301 ccaatgatat caccgcccag aacctccgtg gtgatctcac caatttcgcg aatccatttg
     6361 gccgtcttgc tcgcggcggc cacgatggaa gtctcttcaa cggccatagg aatgatatag
     6421 tctttgccat tgatgcgaaa attagtggcc actcccaagg ggacttgaaa gtaaccaatg
     6481 acattttcaa taaatttttc tccgagggag gtgtctttca gtcctccttt ttcaaggaag
     6541 tcgacatcac gaggttccaa agctcccgtt tcttcaagag cttctaaacg ctcccgacgg
     6601 gtgagctttg aaaagccttt aaaaatatca cttaatgttc gtgccatttc caaaccctct
     6661 tcgtcctgaa gtcttccagc ttttgcacac cggtgcaaaa catggctatt ttcacttcaa
     6721 gtaataattt ttcatagagc acattgacag cttcagagct ttctaaggct gccttcaacc
     6781 aaggctgagc cagacccacc atgtctgccc ccattgccag gagcttaaca gcttgaagcc
     6841 catcgcgcac gcctccagag gcccacactg gctttttttc atcaatttct ttagcatcca
     6901 gcaaactttg aacagtcgaa agaccccagt tttcaaaagt ttgcgcaatc tgaaataaaa
     6961 gctcctctgg ctgagagcga tatccctcaa cccggcccca gtgggtgccg ccgagaccac
     7021 tgacatccac agcaaatact ccggaattgt acaagcgttg caaagtccct ttagaaaatc
     7081 cgcatccgac ttctttgaca atcaccggta ccgatagttt tttaaccagg gactcgatgg
     7141 ctgcgtagcc tccgcgaaac tccggtgtcc cctcgggctg caaacactcc tgcaagggat
     7201 tcaaatgaat aaataaagct tccgcctcaa gagagtccac taaagcctga acacaatcca
     7261 caggcgtctg aatcacttgc gcaatcccta cattccccag aagtaaagcc tttggagctt
     7321 gctgacgaat tttcttccac tccagctgcg cttcagaatc catcaattca cgacgctggg
     7381 agcctactcc catgagaatt tgtttttcag cgctcagttg cgccagtctt gaattgatat
     7441 cgaaggcttt ttcatgccca gccgtcatag agctaataaa gaaaggagcc gaaagtggtt
     7501 tgctcatgcc acgggaaaaa aaagaggtcg acgcatcgac ctctttaaag ttcatattag
     7561 gcaaagcttc atgaatgagc tgaacttgac tgagacctga aagaccttcc gcctgggagc
     7621 gaggatctaa ggcgattcga atatgatcct gcttcctttt ttcgaatgat tttttttcga
     7681 atggctttga agactcagac attttcgctc accttaagct ttaaagctta agcaagcgca
     7741 tgcttgcaaa gagctgtgaa tgccgcagca tcatgaattg caagatcagc catcacttta
     7801 cggtcgacaa ggattcccgc ttttgtcaaa ccaccgatca aacgagaata agtagttcca
     7861 ttcaaacgag ccgccgcatt gatacgttga ttccataaag cacggaagtt gcgttttttc
     7921 atcttacgat gacgataagc atattgtaat gcgtgatcgt ttttctctac cgcatggata
     7981 tagcagcgag aacctgctga gtaataacct tttgttcttt ttaatacttt tttgtgacga
     8041 gcgcggtttg ttttaccact ttttacacga gccattttcc actccgatta tctattaatg
     8101 aggttagacc tagaacacca acaaacgctt cacttgaagc aagtttgcag tttctacgta
     8161 agaagtttta cccaattgtc ttttaacttt tgagctcatg tgggagttca aatggcgaag
     8221 acgtgtgctt ttctttttga ttttgccgct ggagagcagc ttaaggcgct ttttagctcc
     8281 tgaatgcgta cgcattttca tagatatctt tcctccgata tggcgtctta ggtgaatacc
     8341 caagctcttg ttaattgcct ttatcagcga taatgagtgg tctgttttaa gacgtggcag
     8401 gtcgaaaatc aagctctttt aagggtttaa ctcgctttta agtcaaatat atacgatctg
     8461 aggaggactc tatcttacca caggctctct tcactaactt tgaacctaaa cgaatttata
     8521 aagtatcttc acccaagccg cacagcccag ggcaaccaac agactgctga gctggggctg
     8581 aaaaagaaaa gcacttcccc ctacgagaaa gaataaaccg taggggaaaa cctgccaaaa
     8641 ataagaatcg tctttgaaag aaggctttgg agctttttta gagaaccact gcggacaaaa
     8701 gaaaccgaaa taaaggaggg atattccaaa gactatggaa atgtaagata acacacgttc
     8761 atagcttata tctaacaagt gactggctaa tatcatttat gaacctcgct tttaaaactt
     8821 aaaagcgaag tcagaaaaag ccaagaaact ctcgtctaga aaatacgatt tttagacgag
     8881 agcgcaaaaa caaataaata aaaaatagat ccagagcaga actattctgc cacttcatcc
     8941 tcttcttctt cctcctcatc cttaagctcc gcaagctctt tagtatcttg cttagatttg
     9001 tttggatgag cctttaaata gtcgcgaatt ttgagaggat ctggagcaat cagaataaac
     9061 atctgtttac cttccatttt cggattggtt tcaatagtcg ccaaatcctt aacgaactcc
     9121 atacatcttt tcatgatgtt catgccgatt tcttgatggg ccatctcgcg gcccatgaaa
     9181 cgcaaattca ctttcacctt atcgccatcc aaaaggaaac ggcgagcgtg attcatttta
     9241 gtttcaaagt cgtgttgatc tgtgcgcgga cgcatttgaa cttctttgat gctgacgatg
     9301 acttgttttt tacgagccgc cgccgctttc tttttgttct cgtacttcca cttgccataa
     9361 tccatgattt tacaagtagg aggtgtcgct gtaggcgcaa tttcaagaag atcgaggttg
     9421 cgttcttcag ccaatcttaa cgcctcaggc accgtcataa cacccaacat atttccttca
     9481 tcatcaatga cgcggatttg gggagcgcga atttcgcggt ttactcttaa cgagtcttta
     9541 gagtcttttt taccacggaa accagctccg cctctaaaat cgtttctgcc ttcgaacttg
     9601 ctaatgggaa acctccaggg tttttagatc ttcatcaaag aagactgcag tttgcgttcg
     9661 ttaatatctt cagtaaccaa tttcattaaa tcatctaccg caatgccttt gtgctcagag
     9721 ccgtctctca gacgcaaaga caccgttcgc gatgtcgcct cgctatcgcc aacgatgacc
     9781 atgtagggca ctttttgcaa ttgagcttca cggatcttaa acttcaattg ctcattgcga
     9841 ttgtcaaact caacacgcac ctgactgtct ttaaacatct gctgcagctc ttcgcagaag
     9901 agattgaccc gatctgtgat atttaaaatc atcacctgca caggagccaa ccacggcggc
     9961 aaatgccctg cagtatgctc aaggtaaacg ccaataaatc tttcgagaga acccaagatc
    10021 gcgcgatgaa gcatgactgg gcgatgttct cgattatcga ctccgatgta ttctaactgg
    10081 aaggcctccg gcagattgaa atccacctgc aaggtcccca attgccaagg gcgattgagc
    10141 gcatccacga acataatatc caatttagga ccatagaaag ccccatcacc tggattaatg
    10201 gtaaagggca gattcaaact gttcaaagca gtggtgagag ctccctcagc tttatcccaa
    10261 acctcttccc cccccatgcg gttttcagga cgagttgaga gatagatttt gtaattactc
    10321 attcccaatt gcgtgtagac acgattgaga agttgcatga agctggcgat ctccgtctgc
    10381 atctgatcca tcatacagaa aatgtgggca tcatcctgac agaatgttct cacccgagtc
    10441 agaccgtgca tagcgcccga tttctcgaag cgatgcagac gtccgaagtc cgccattctc
    10501 agcggcagtt gtttgtaaga atgaggctcc gccttgaaca gcaagcagtg agaagggcaa
    10561 ttcatcggct tggttgcaaa gtccctctca tccacgcgag tgaaaaagat attgtctttg
    10621 tagttcgcca tatgacccga agttataaat aaattcgagt caaagatttg gggagtgatc
    10681 acttcttgat agccaaattc aaagtacaac tcacgcatat aggtttgcag ctgagtatag
    10741 actgtcgctc ccttacccgt gaaaaacgga gagcctggag cccactcatg aaaatgaaaa
    10801 agacctaact ctttgccaag tttacggtga tcgcgttttt ttgcttcttc gatattatga
    10861 agatattgtt cgagttcttt tttatctcca aaggccgtcg cgtaaatacg ctgaagcatc
    10921 ggattctttt catcgccacg ccaataggca ccggcaacag aaagaagctt gaaggcttta
    10981 atttgacctg tggactgaat atgcggccca cggcaaagat caaaccagct atcgccattg
    11041 aagtaaatcc ccacttcggt ctcacctttt tcagctaagc cctcgataag ctccactttg
    11101 aaacgttctt ttagaccttt gaaagtctca acggctttag cgatgggcca attttggcga
    11161 gcaatgggaa ggtccttagc gacaatctca ctcattttct tttcaatttg ggcgaaatgc
    11221 tcttcggtaa aatgaaaagg ggaatcgaaa tcataataga aaccattctc gatgacagga
    11281 ccgatggtta ctttcacctc aggccaaata tcttgaactg cttgagccat aatatgagca
    11341 caagagtggc gaatcacttc agcggcttct ggagatttgg tggtgatcaa agccactttc
    11401 g
//
LOCUS       DATBTE010000143         2606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1793569, whole genome shotgun sequence.
ACCESSION   DATBTE010000143 DATBTE010000000
VERSION     DATBTE010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793569"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..2532
                     /locus_tag="VIG33_05330"
     CDS             292..2532
                     /locus_tag="VIG33_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270289.1"
                     /translation="MYAVKILKLGCRQSMIFLVLFSFQAPPVLALPGIISEDAGLLPI
                     EKSTVNQSLIDELFNPAPVSNNWGANSFYIPQNPGNNQNHGNTRITTTSPGMKKSNVK
                     DEFKCNLFENVPYEDILSALNSLNQAVSSPACGGPSQTNVQGIVNNNKTIADAIKDLR
                     GFVENPGSIQPENAADISNKVDLAIRAATTIANSFAQSDLLSKECRQAMSAGQVATSI
                     NDLINGLTPYALMAATMTTGGAAAVPYIVGGSIITGAIGSMNKIIDENGVQVEQPEVR
                     RAIVENTCQFIRLDQKYKFLIKSRDEQISKITTDLSSSKRLFSAKLGVQGSKNAVEKF
                     LTEIDTKIPVARSQLEMDKQFMRSTSDDLKICQLGIQLTVMAQDKASYVSTMLTSLDK
                     ALAIYGTNNVAQAQALKLSGTLAAKDLRSVAVSQFSSKANYKKCAESTKSFIETIGQS
                     ASLAKELVTLAQENLEKGLQGDKEYNLYKYHLLTLNQKQFQAQRVTDSLDKLQAYATT
                     ITQAEIDSEMDRLRRGLFNNSYMGMAPSPVLKWFKFVNGLHREDLSRFKDGLSSLRLR
                     AYQMSPSGKAHKSIVGFYAKDSKDGKDAANLVQFNLKDLPMGTPDHDDICREMRDVWN
                     RWTSAIDYLAASDAFCNMLEPYVYDNRPEDRSLVAMCRGYSKSSPIAIHGQAILSTVA
                     NMKNRLVVDKTRDWALYLQKKIEALNCVDNFSVETPKDGNMPKSGSPKKISVLFEQPE
                     FFRCQS"
BASE COUNT          746 a          532 c          613 g          715 t
ORIGIN      
        1 gagtgggata cacctacacc aatgaagaat cctttaaaaa taagatctta caacggtgtg
       61 gattcataaa ctcaagaaac ttaaatacaa atggatggta ttggcatgta cctacacccc
      121 actaattgat agaaaggcaa cggagaggcc ggtcagaaat aggcattttt taggtcgttg
      181 tctaagtttg agacagtgat tgcgccaggg cctccactat atgtcaatag atcttggtat
      241 tatccgtgca tgcttcattt tggcatagtt gcgttcccaa ggagttcttt tatgtacgca
      301 gtgaaaatat taaaacttgg ctgtcgacag agcatgatat ttttagtgct tttcagtttt
      361 caggctccgc cggtattggc tttaccaggg attataagtg aggacgctgg tcttctgcct
      421 attgaaaaat ccacggtcaa tcagagcctt attgatgaat tgtttaaccc agctcctgtg
      481 agcaataatt ggggggcgaa ttccttctat attccgcaaa acccagggaa caatcagaat
      541 catgggaaca ccagaattac gaccacttct ccagggatga agaaatccaa tgtgaaggat
      601 gagttcaagt gcaatctttt tgaaaatgtt ccttatgaag acatcttaag cgctctcaat
      661 tctttgaatc aagccgtgag ctctccagca tgtggcggcc cctctcaaac gaatgtgcag
      721 ggaattgtga ataacaataa aaccatcgcg gatgcgataa aagatcttcg aggttttgtt
      781 gaaaatccag gaagcatcca gccagaaaat gcggcggaca tttcaaataa agtggattta
      841 gccatccgag cagccactac gattgccaac tcttttgcgc aatcggatct attgagtaaa
      901 gagtgtcgcc aagcgatgag tgctgggcaa gtggcgacct cgatcaatga tttgatcaat
      961 ggtttaactc cttatgcttt gatggcggcg accatgacca cgggaggcgc tgcggccgtc
     1021 ccttatattg tggggggcag tatcattaca ggtgccatcg gaagtatgaa taagatcatc
     1081 gacgaaaacg gcgtgcaagt agagcagccc gaagttcgtc gtgcgatcgt agaaaatacc
     1141 tgtcaattta tccgcctcga tcagaaatat aaatttttaa ttaagtcgcg ggacgagcaa
     1201 atcagtaaaa tcaccacgga tctttcctcg tcaaagaggc ttttcagcgc aaagcttggt
     1261 gtacagggaa gcaagaatgc tgtggagaaa tttcttaccg aaatcgatac gaagattcca
     1321 gtggctcgtt cgcagttgga aatggataag cagttcatga ggagcacttc tgatgatctt
     1381 aagatttgtc agttaggtat tcagttgacc gtcatggctc aagataaagc ctcctatgtc
     1441 tccacaatgt tgacttcttt agataaagct ttggctattt acgggactaa taacgtggct
     1501 caagcgcagg ctttgaaact cagtggaacg ttagcagcca aggatcttcg ctcagtggcg
     1561 gtaagtcagt tttcttctaa ggctaactat aaaaagtgtg ccgagagcac aaagtctttc
     1621 atcgaaacaa taggtcagtc cgcctccctt gcgaaagaat tggtgaccct tgctcaagag
     1681 aatcttgaaa aaggcctgca gggcgataaa gaatacaacc tctataaata tcatttgcta
     1741 accttgaatc aaaaacagtt tcaggctcaa cgggtcacag actctctcga taagctacag
     1801 gcttatgcga ccaccatcac tcaggctgag attgactccg agatggatcg actccgtcgt
     1861 ggtcttttta ataatagcta tatggggatg gcgccttcac cggtgctgaa gtggtttaaa
     1921 tttgtcaatg gtttgcaccg cgaagacctc tctcgcttca aagatggtct cagttccctg
     1981 cgccttcgcg cttatcaaat gagtccttcg ggaaaagctc ataaatctat tgttggcttt
     2041 tatgccaaag attcaaaaga tggaaaagac gcggcaaatc ttgtgcagtt taatcttaaa
     2101 gatttaccga tggggactcc tgatcacgat gatatctgcc gcgaaatgcg ggatgtttgg
     2161 aacagatgga catctgctat tgattatttg gcagcttcag atgcgttttg caatatgctt
     2221 gaaccttacg tgtatgataa tcgtcctgag gatagaagtt tagtagctat gtgccgtggt
     2281 tactccaaga gttcgccaat cgccattcat ggacaggcca ttttatcaac cgtggcgaat
     2341 atgaaaaaca gactggtggt tgataagacc cgtgactggg ctctctattt acagaagaaa
     2401 atagaagctt tgaactgtgt ggataatttc agcgtggaaa ctccgaagga tggaaatatg
     2461 cctaaaagcg ggtcgccaaa aaagatttcc gttttatttg aacagccaga attcttcaga
     2521 tgtcagtcct gatagcaaga gggccttgtg tggcggaagg tgtttgaaga attttttaaa
     2581 cttccgcatt tcatttcgaa ttgttt
//
LOCUS       DATBTE010000144         4431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1862680, whole genome shotgun sequence.
ACCESSION   DATBTE010000144 DATBTE010000000
VERSION     DATBTE010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862680"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1167)
                     /locus_tag="VIG33_05335"
     CDS             complement(19..1167)
                     /locus_tag="VIG33_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270290.1"
                     /translation="MNDVQEISEQNTQYDKGVGGNLFLDEEKSNSKGDGGNVFMNFEG
                     SDNRLEEPNSVVIDLWQKLKEPKTQDSIVDLSQVPNVEEIPGKLGDLSVGPSLVEETC
                     DDSEADDRTLNIQNSKMAEQESDAFIASDLNDSDSDDERTVAVEGFANRQPNAQKSVS
                     AKISFGSLREPRPAASGGGSVYASSDANLAQAENLKLAQRRILDLEKEVERYRQENEE
                     LDSAGDIIRNRYEELTAKFFILEKEKQEVSDSLQNELLILKGTLQHKETEISMSRLKI
                     EELETRLKSDFKKIRVRERELENRLELVKAEKTALLRAKDENILELKRKIDHVQSELD
                     NYRHKVLELNKALGLNHEQFKRTVRALRLALSNLEVKEENLAPLKKAD"
     gene            1435..2880
                     /locus_tag="VIG33_05340"
     CDS             1435..2880
                     /locus_tag="VIG33_05340"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEY8270291.1"
                     /translation="MSLVSVLMTVGIGAIILAGITSMITDVFKAQRTIQSKDAHRELI
                     SGIRQLLTDPAICSTSFSGGDPAGTGFTRTQIVDTATPPNIKYQIGTNYLNNLVTLTG
                     FEIKNFTADNVAVNPKLGKAELNLRMNKIGSTIGSVEMTTTIFLRTTLDAADKLNSCY
                     ALGIADSLWQISPTNMADIYYSSGNVGIGTSSPGTALHIHNATDKNLLFGLSSNIAGG
                     MEIVSSNDAIDTPLPLELRGTPTIFSIGNLGEKMRLDASGNVGIGTANPSSTLHVVDN
                     MNYYGITSQSSAAQGYAIHGVGPNGLSADAFLASAAGITTTNSATGSIAQLAYQGWGV
                     YCQAGLCGGATGQWQVQSDRRLKHQIFTIDNALEKILKMRGVYYHWRNPETDLKEGQK
                     VGFIAQEVEKVFPQAVNTVKMPSGDGANFPEGTKVLAYSDLVGPIVQAIKELHAKWLS
                     DHEQIVQLKAENIILKKQNDEILRRLELLEQ"
     gene            complement(2946..3854)
                     /locus_tag="VIG33_05345"
     CDS             complement(2946..3854)
                     /locus_tag="VIG33_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEY8270292.1"
                     /translation="MSDILVVGSLAYDSIETPSGRVDRALGGSANYFSLAASLFAKVR
                     VVGVVGEDYEESDYKLLQARGVDLEGLEKVSGKTFHWEGKYEGDMNEAQTLRTELNVF
                     EHFNPKLPESYKNSAFVFLANIVPALQLEVLNQVKNPQFVGMDTMNLWIDIANADLLK
                     VLKKVDVVLINENEAEMLTSADNAIAAAAKIVELGPKAVVIKRGEYGFIMYSKEEGYF
                     VLPAFPIAKVTDPTGAGDTFAGGFFGYLASLPNKPTVKEMKKACIFGSILASHTIQDF
                     SVKALAKTTLADLEKRMVEYQKVITV"
     gene            complement(3817..>4431)
                     /locus_tag="VIG33_05350"
     CDS             complement(3817..>4431)
                     /locus_tag="VIG33_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY8270293.1"
                     /translation="DNPEHVFKSIEVLQNPWGMMLSRRKITVSTSGLVPQIKLVSDAR
                     VRLAVSLNGSNDEIRSRVMPINKKYPIKELLDACREYCRVTKDKITFEYVLLKGVTDQ
                     LEHAKELVKLLKDVPSKINIIPFNEHPGSGFERPSDEQIQKFHTELIRLGAHVLLRRT
                     MGRDIYAACGQLTTVKDRPETMDISNSKIGGQNERHFSSGQSSL"
BASE COUNT         1202 a         1025 c          891 g         1313 t
ORIGIN      
        1 aagtgatttg ttccatgttt aatcagcttt ctttaaagga gcgaggttct cctctttaac
       61 ctctaagttg ctcaaagcca agcgcaatgc acgtaccgtg cgcttaaact gctcatgatt
      121 aagaccaagc gctttattaa gttctaaaac cttatggcga taattatcta attcactttg
      181 aacatgatca atttttcttt taagttccag gatattctca tccttagccc taagcagcgc
      241 agttttctcc gccttaacca gctctaaacg gttttccaat tctctttcac gcacacggat
      301 cttcttaaaa tccgatttaa ggcgagtttc aagttcttca atctttagtc gtgacattga
      361 gatttcagtc tctttgtgct gcaaggtgcc tttgaggata aggagttcat tctgcaacga
      421 atctgacact tcctgctttt ctttttctaa tataaaaaat ttagccgtca actcttcgta
      481 gcgattgcga atgatatcac ccgctgagtc gagttcttca ttttcctgcc ggtatctttc
      541 aacctctttt tctaaatcca atattcttcg ttgcgcaagt ttaagattct ccgcctgagc
      601 gagattggcg tcactcgaag cgtatacact cccgccaccg ctcgctgctg gacgaggttc
      661 ccgcagcgaa ccgaaactaa ttttagctga aacacttttt tgtgcgttcg gttgacgatt
      721 tgcgaagccc tcaacggcca ccgttctttc gtcatctgaa tcagaatcat taagatctga
      781 agcgataaag gcatcggatt cttgttccgc catttttgaa ttttgaatgt ttaatgttct
      841 atcatccgcc tcagaatcat cacaagtttc ttcaaccaaa gagggcccta cagacaagtc
      901 cccgagcttt cctggtattt cttctacatt tggaacctga gaaagatcaa caatcgaatc
      961 ctgggtctta ggttctttca gtttttgcca aagatctata accactgaat tgggttcttc
     1021 gaggcgattg tcacttccct cgaagttcat gaaaacattc ccaccatcac ccttggaatt
     1081 acttttttcc tcatctaaaa ataaattgcc accaacgcct ttatcatact gcgtgttttg
     1141 ctcactgatc tcctgaacgt cgttcattag cgactcgatc agatcatctt taggtgtaag
     1201 ttttttcttt ttcttagcca tgacttgagt taactatcgg aaaatagaag atgtttcttt
     1261 aagaaaagat cgcggtttga gagaaaatac aaccttagtc cagtccagct ttgaaatgtt
     1321 gtctccctga ctgaatattt gagatatgac tggaaatttt caatttgttt acgttaagtc
     1381 gttgcccttt tcgccgatct taataatatg cagaatttat ataatcaacg gggtatgagc
     1441 ctcgtaagtg tgcttatgac cgtaggaatt ggtgcgatta ttttagctgg aatcacctcc
     1501 atgatcacgg atgtttttaa agcccagcgc acgattcaat ccaaagacgc ccaccgagaa
     1561 cttatctccg gtatccgaca gcttttgaca gatcctgcca tttgctctac aagtttcagc
     1621 ggtggagatc cggccggaac tgggtttacc agaactcaaa ttgtggacac ggccactcct
     1681 ccaaatataa agtatcaaat cgggacaaat tatctgaaca atcttgtcac ccttaccgga
     1741 ttcgaaatca aaaatttcac tgcagataat gtggctgtga accctaaact cggaaaagcc
     1801 gagcttaatt taagaatgaa taaaattggt tctaccattg gtagcgtgga aatgacgaca
     1861 actatttttc tacgaacaac tttggatgct gctgataaat taaatagttg ttacgccctt
     1921 ggtatcgcgg actcactttg gcaaatctcc cctactaata tggctgatat ttattattca
     1981 agcgggaatg tagggattgg cacatcgagt ccgggtacgg ccctgcatat tcacaatgca
     2041 actgacaaga acttattatt tggattgtct tctaacatcg ctggtggaat ggaaatcgtt
     2101 tcgagtaatg atgccatcga cacaccatta ccgctagaac ttcgtggtac tcccactatt
     2161 ttttcgatag gcaatttagg ggagaaaatg agactcgatg cctctggcaa tgtgggcatc
     2221 ggaacggcaa acccttcttc tacccttcat gttgtggaca acatgaacta ttatggaatc
     2281 accagtcaat cttcggctgc acagggatat gcaatccacg gtgttgggcc taacgggttg
     2341 agtgcggatg cattcctagc ctctgcagcc ggaataacca cgactaacag tgcgactggg
     2401 agtattgctc aattggcata ccaagggtgg ggtgtttatt gtcaagcggg actgtgtggg
     2461 ggagccacag gtcaatggca ggttcaatcc gaccggagac ttaagcatca aattttcaca
     2521 attgataatg cactcgaaaa aattctcaaa atgcgaggag tttactatca ttggcgcaat
     2581 ccagagaccg atctcaagga aggccagaaa gtgggcttta tagctcaaga ggtcgagaaa
     2641 gtcttccccc aagccgttaa cacagtcaaa atgcctagcg gcgatggcgc caattttcct
     2701 gaaggaacaa aagtattggc ttatagcgat ctcgttggtc ccattgttca agcaatcaaa
     2761 gaacttcatg caaaatggct cagtgatcat gaacagattg tgcaacttaa ggctgaaaac
     2821 atcattctta aaaaacagaa tgacgaaatc cttagacgtc tggaattatt agaacaataa
     2881 aattataatt taccgcaata agaaatagaa gcatgagtga acgcttgatt cactcatgag
     2941 tcctcctaaa cagttattac tttttgatat tcgaccattc gtttttcaag atctgccagc
     3001 gtagttttcg ccagcgcctt cacgctaaag tcctgaatag tgtggctcgc taagatactg
     3061 ccgaaaatac acgccttctt catctctttg actgttggct tatttggaag gcttgctaga
     3121 taaccaaaga atccacctgc aaaagtatcc ccggctccgg ttgggtctgt tactttggca
     3181 atgggaaatg cggggagtac aaaatagcct tcttcttttg aatacatgat gaagccgtac
     3241 tcgcctcgtt tgataaccac agcttttgga cccagctcaa cgatttttgc agctgccgca
     3301 atcgcattgt cagcggaagt cagcatttcc gcttcatttt cgttgatcaa tacaacatcc
     3361 acttttttca gaaccttcaa aagatccgca ttcgcgatat caatccaaag attcattgtg
     3421 tccataccta cgaattgtgg gtttttaact tgattcaaaa cttccaattg caatgcagga
     3481 acaatattgg ctagaaaaac aaaagcggaa tttttgtagc tctctggcaa tttaggattg
     3541 aagtgctcaa acacattgag ttctgttcgc aatgtttggg cttcgttcat gtcgccttcg
     3601 tattttcctt cccaatgaaa ggttttgccg gatacttttt ccaaaccttc aagatctaca
     3661 ccgcgagctt gcaaaagttt gtaatccgac tcttcatagt cctcgcccac aacacccact
     3721 acgcgcacct ttgcgaataa gcttgccgct aaagaaaaat aatttgccga tcccccgaga
     3781 gcacggtcca cgcgccccga tggagtttcg atgctatcat aggctagact gcccactact
     3841 aaaatgtcgc tcattttgac caccaatttt tgagtttgag atgtccatag tttcaggtct
     3901 gtctttgacc gtcgtcaact gtccacaagc tgcgtaaata tcacgaccca tcgttcttct
     3961 taacagaaca tgggctccga gtctgatcaa ctccgtgtgg aatttttgaa tctgctcatc
     4021 tgaaggtcgc tcgaaaccgg agcctggatg ttcattgaaa ggaatgatgt tgatcttgct
     4081 cggaacatct ttcaaaagct tcaccaattc tttggcatgc tccagttgat ccgtcactcc
     4141 ttttaacaaa acatactcga aggtgatttt gtctttggtg acgcggcaat actcacggca
     4201 ggcatcaaga agctctttga tcggatattt cttattaatc ggcatcacgc gagagcgaat
     4261 ttcatcgtta gatccgttca aactcacggc caaacgtacg cgcgcatcag acaccagttt
     4321 aatttgcggc actaagcctg aagtggagac cgtgatctta cggcgggaca acatcatgcc
     4381 ccagggattt tgcagaacct cgatggattt aaaaacatgt tcaggattgt c
//
LOCUS       DATBTE010000145        18771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1453617, whole genome shotgun sequence.
ACCESSION   DATBTE010000145 DATBTE010000000
VERSION     DATBTE010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 18771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18771
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453617"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VIG33_05355"
     CDS             complement(<1..664)
                     /locus_tag="VIG33_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270294.1"
                     /translation="MKLLLPILIVLLGSHDFARANPAPRVGREVASKYFEKQNSDAGE
                     QGEEADSSSKPSSYSSSGPSDHYLALHFGKMMNSTAYEWGTESKQTNVGDFTTGLTYR
                     VGEWKNSMDLNIRIDFIQFNVTGNGEPKPLKMSILPLITFPDAAAKFPLYFGFGMGPG
                     VFFRQSDGEASLALDYQLIAGARFFDVFENAGFFIESGLKNHLQLWNSGQFNGTFLSA
                     GAV"
     gene            809..1375
                     /gene="nrdR"
                     /locus_tag="VIG33_05360"
     CDS             809..1375
                     /gene="nrdR"
                     /locus_tag="VIG33_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY8270295.1"
                     /translation="MKCPYCNHSEDRVLDTRVQKDGSIRRRRECLECKVRFSTLETLM
                     LSFPFVIKKDGRREPFSKEKILKGLQASCQKRPVSLAQLETIVERIASWLISRGDNEI
                     PARLLGLRVMAELKQLDDVAYIRFASVYRTFKDVQEFVETLEDMELAEVINTNNPQLP
                     LTTVNYSGKPEENEKTIPGTSTTSPLSN"
     gene            1326..1724
                     /gene="nusB"
                     /locus_tag="VIG33_05365"
     CDS             1326..1724
                     /gene="nusB"
                     /locus_tag="VIG33_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY8270296.1"
                     /translation="MRRQSRELALQVLFQIEFAPQISNADLMEVLGASLEQDTVKFAD
                     GLIRGVQVQKTAIDAKIQAVSTHWKLERMATIDRNILRVAVYELCFTPNPLKENIIIN
                     EAVEIAKKFGTTESASFVNGLLDQIAKAQR"
     gene            1721..2167
                     /locus_tag="VIG33_05370"
     CDS             1721..2167
                     /locus_tag="VIG33_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270297.1"
                     /translation="MNVLSLASAFNPGSDLWIIPDPNNCRWTQKIDWYLNFQIVKSQR
                     HLSPETRNFTKLIQKETGLEIYEHTLMKNAPLMMTSEAFFPNKWVIVVPLSANFSAWV
                     REVSNIWENLKHPSLRVFLPTGQNARSFNQEWQNHHSFEDFTLVLD"
     gene            2195..2728
                     /locus_tag="VIG33_05375"
     CDS             2195..2728
                     /locus_tag="VIG33_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase"
                     /protein_id="HEY8270298.1"
                     /translation="MNIITHFQKRIESILDNELDAPSEEVLLAKKHFEALTDPRLLRG
                     LAESLPQDSTDKAIILFSRLALYFDAGVFVEADKNLWEPQAHFHRGHMSVLKTPQKKS
                     ISLPKVDLMKVLRTDTKPILQKLHLEKLDTENKTVCLLLRPGVDFTFLLFSSLPDLWL
                     KEHMRLVTEAIHHGIHN"
     gene            2728..3561
                     /locus_tag="VIG33_05380"
     CDS             2728..3561
                     /locus_tag="VIG33_05380"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEY8270299.1"
                     /translation="MTTWTIYILSDGTGETAATMIRAALVQYANKDINIVRCKNVRTE
                     DQVEAIIEECFERRGFIAYTVAGPNMRKKIHELANSKGLPSFDLLGPLLNTLDNFFGD
                     ANTSTVNVLRTVDDTYFKRIEAIEYTVKHDDGKTLSHLDKADIVLVGISRTSKTPLSI
                     FLSHKGWKVANVPMVLNTPLPEELFKVDQRRVVGLIIDIESLHRIRKNRLEKFGQDTG
                     GEYASISHIVKEIEYAHQIFKQNKRWPVFNVTERALEETAGEIVRIISARLGLPDSVL
                     F"
     gene            complement(3518..4024)
                     /locus_tag="VIG33_05385"
     CDS             complement(3518..4024)
                     /locus_tag="VIG33_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270300.1"
                     /translation="MKFIITMLFVLSPVLSLAGQFAAKCIPQVDSCSYYLCEEKNHPC
                     GPKGYPLGFGFKICQIFLNSEQNYSTEARNWLRKVRVCLMQEFPEADDSVTRTCGEIK
                     TTSFHSHVGCYISTGFCELSIAEQMQIFWPMRGSLIYTEVLRDAYSIAQVCAFRTTLN
                     PEALSALR"
     gene            complement(4198..5280)
                     /locus_tag="VIG33_05390"
     CDS             complement(4198..5280)
                     /locus_tag="VIG33_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270301.1"
                     /translation="MFIKQMILMASLLLTMVSAKAERFWIADPGIPRFCMQNFNAYGP
                     LYAFHMEERTQGLMAELYAKPICTIVHLQEVWNSPQIDMIESSLRRYYNISAPNRLEK
                     IGVMSLMMADIKGTETHDFKVNSEGNVLDLVRGAFNVKKAYHVVKASLTGIDEDFYFV
                     NTHLHPTNSAVRLTQILDLLDWRLAHTDLKLLLSGDFNGDENSLERAVIMLLLGVRDS
                     LVETLGGTYPKGVCTYCESNPLGWLAGDHVLDYIFFSNIGGDATQLKPFDGSINLGGT
                     PRKPLSDHYGLRVHFAVESIKTKADQSNIEQRRALALRFFAHIDRILGAEKKKEFRVY
                     LQKVRGLRAQLVEHQGAFNTYFELFN"
     gene            complement(5368..6273)
                     /locus_tag="VIG33_05395"
     CDS             complement(5368..6273)
                     /locus_tag="VIG33_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8270302.1"
                     /translation="MMSLYQLTTFVTVISEGSMTAAADKLYLTQPAVSQQIRNLEEDL
                     GVELLVRGVRQIKASPQGEVLYEQARKILQLAQQTEIAIKSIGAELKGLIRIGTLNSI
                     GLHIMSPLIGRLMRHNPDLQIKVEYKTGEDLVKGFKKGNFDVLILPQLQEEFGLELDN
                     IEEKFLLKEEMWLVVSGKETDMPSQIAAKELSHFPLVNFVDEFPGFNAKLQKLMSGVG
                     INPKPIFESSNVGTLKRVIESGLGWGFLPAHSIRKQVRSGRLHHVHVKEFHYEIDMTY
                     YFRKTSEARQLAEILYQTIATQEKA"
     gene            6348..7076
                     /locus_tag="VIG33_05400"
     CDS             6348..7076
                     /locus_tag="VIG33_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEY8270303.1"
                     /translation="MKYQFFKYPLITLLFILLTSAAHAKVFRNAYISFELPPSWNCSL
                     EQTEWVCRSESSKEAKEAIIILTAKEVGPTDSTQLYESHLNSPITLNLKTGGTSESKV
                     VYKAKTVQINDQNWIDGLHMGSEVTNYFTRYLATIKGRIAVLVTFSAHKQFYTKYSQD
                     FFKAVMSLRVIETKNLANNPEIRPGAGTLGPGSFQNSMPADMSNPDDMQTNSGKGSKM
                     MLLGIALILAAAGVYLFLKKKRKK"
     gene            complement(7100..8530)
                     /locus_tag="VIG33_05405"
     CDS             complement(7100..8530)
                     /locus_tag="VIG33_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY8270304.1"
                     /translation="MRKPQMKILLNLIMISLVGCVAPEKKTEIVKVNKTPVVVMPMES
                     QFKLRPMREMQLENGLKIIFIADNSLPRVSMTMLVKVGARQDIKGKEGINNLTVQFLE
                     QGIQIKNAMVLADEMGQLGTEIGIETGNDFTIITMDALVNSADKLLSLYSDIIMNPAF
                     MDVEVARAKSQVIAQQQKKIDNPSNYANDAFDEFLYQGHPYANDITGTPGSVKRITKQ
                     DIIRHFLNNYRPNNAELAVVGRFNKDFEVKVSEAFKKWSGKPIKEFATPELKPIQSLE
                     LKLVSKPGLQQAQIRMGEFGIRRNDPDFLKLRLANVALGGEFGSRLNQHIRDDLGLTY
                     SIYSSFDSRADRGPFLISTFTKNETVGKVVSESLKVLSEFVDKGITEAELKASKEQML
                     GQFPRAIETADRLAYNILVLNFYGVPLSYLQDFTKNVEALSLSEVNKAIQKHLDGKNL
                     RVLVYADEKKVADQLKDYKPILEKLK"
     gene            complement(8534..9868)
                     /locus_tag="VIG33_05410"
     CDS             complement(8534..9868)
                     /locus_tag="VIG33_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY8270305.1"
                     /translation="MKQLLFFFSILFIISHAHGQEKDNLKINFQVTKYKLQNGLTVLL
                     HEDHAVPLITYHTWYKVGSRDEAPGITGSAHMLEHMMFKGSKSYPGKSFDRLLHENGI
                     VNNAFTTYDYTGFYQSLPSSKLEMMMEIEVDRMNNLNLNPEDLQSEREVVKEERRWRV
                     DNNPMGLLMETVMNTVFKTANYRWPVIGTMKDISDYKIETLRNFHSTYYVPNNAILVL
                     AGNFKTAEAKRLIEKYYGPIPYRPLPRREYPREVVQVTQRNAKVKKDVQGISFTVAYG
                     SVPNAHAEMYPLDLAANILGNGSSSRLHKRLVYTKQSATSAYAYNSTMQDQGVFAVGI
                     NLKPGMPMEENLELVYQEIWKLRNKLVSDEELAKAKTQVMKELVDGLTTIDGKARALA
                     VNEIMTGSYENLFKDLNKYNAVTALQIKESASKYLNQNQRSIVVLESQGKKE"
     gene            complement(9955..10467)
                     /locus_tag="VIG33_05415"
     CDS             complement(9955..10467)
                     /locus_tag="VIG33_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEY8270306.1"
                     /translation="MASDKQERPPAKVKNLGLILTVAFAVLNMGIMGGGAYLVYASTL
                     GWQSPKITEEMLETTMASKSEDERFAPYIFTMDKFTVNLGGEPKRIIRLEVSLQMLGK
                     EGYEEIMSPDSRAKARDKIVRVLNDKTFSEIESIQGKLFLKDTIAMEVNTILQEGVVK
                     DVFFSDFVIQ"
     gene            10651..11679
                     /locus_tag="VIG33_05420"
     CDS             10651..11679
                     /locus_tag="VIG33_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY8270307.1"
                     /translation="MTRKLYTWIVSYVAVTASWGLGGLIPSLALAENEAASTSQNKTT
                     SELLPSDLLQISNTDAFSKYVFLVDKTERHLQVFERKGETIEKILDVPSDMGKNVGNK
                     IKRDDARTPEGIYFFEKKLNQPEIPFGLYGELAFTTDYPNIFDRRESKTGSGIWLHAV
                     PDTVPLTRGSRGCVVVRNEVIRKLQEYIKLKETPILIFDKVNYVPKEEHDKRRSALND
                     FIEGWRKAWQSQNIDQYMTYYDQNFKAPGFNYASWKAHKNRLKNHYEFIKVELSQPFL
                     LLHKDQLIVKTFQRYESNNHADFGVKTLYAVKTDSGYKIIREEWVPVSENLTTSTALS
                     TQPTAPNN"
     gene            complement(11686..11883)
                     /locus_tag="VIG33_05425"
     CDS             complement(11686..11883)
                     /locus_tag="VIG33_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270308.1"
                     /translation="MSLDKAMKNLKFDKRLTEWYINNGQLTKEELEAHLKTLPDMGHN
                     VELADSQDEADESDEEEFSQH"
     gene            complement(11922..12680)
                     /locus_tag="VIG33_05430"
     CDS             complement(11922..12680)
                     /locus_tag="VIG33_05430"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY8270309.1"
                     /translation="MNRGSQMSMAVPLTPVVKWLVIINVAIWFIFQILVEGFFKVPFT
                     SFLNLVPSRVIFQFHVWELFTYMFLHSMQVTHILFNMLMLWFFGAELEMRWGGKYFLF
                     YYLFTGVGAAILYCLGMASYAAVTGSQIGLMVPVIGASGAIFGLMLAQGILFGERIVY
                     FFMLFPMKTRYFVMLMGGVQMASMLTSGVSGGEVAYLAHLGGLASGYVALLLMSRWKN
                     YVNFKKTKKKGRNLRLVVDNENPKKSNNGPRYWN"
     gene            complement(12677..13294)
                     /locus_tag="VIG33_05435"
     CDS             complement(12677..13294)
                     /locus_tag="VIG33_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEY8270310.1"
                     /translation="MAKKTALKKKNKISGSKVSSIKMPKVFLKISKDLWPLERDVLFR
                     PDRFKYVRKLIKPEACVFCSAAKNEPSFETLCVYKTKYSMIVLNKFPYNSGHLLVLPL
                     KHQGNLLALSKEAHYDLQDTLRLAMKALEDIYQPGGVNVGLNHGAVAGAGIPDHIHYH
                     VIPRWSGDLNFFPLIAETKVVVESLEQTYDGLLGYFGRKSVGESL"
     gene            complement(13303..14274)
                     /gene="sppA"
                     /locus_tag="VIG33_05440"
     CDS             complement(13303..14274)
                     /gene="sppA"
                     /locus_tag="VIG33_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEY8270311.1"
                     /translation="MKNPFVRFIIVILVIVGLVSVVKWVSSFAGEKDEESALVSKNSI
                     LQLDLEGVIMNGKKFMKKLKKYKSEDKIKAIVININSPGGAVGPSQEIYAAIKAVKEE
                     TKKPIICSSSGLLASGAYYSAVACDKIVVAPGALVGSIGVIMEFANLEKLYEWAKVSR
                     FSINSGKFKDSGAEYRAMRADEKALFQELIDDVYGQFKQAVIDGRKLNPEVVSQYADG
                     RVMTGKQAVQLGFADSLGTLEDAIKAAAEAAHLGSDYKLFEVPKKRPGLWDLIEQQED
                     DDLNSIDEIARVFSKNMGDKSMESSIRKVFRAQYLNQPMLIMPGFWE"
     gene            complement(14385..16187)
                     /locus_tag="VIG33_05445"
     CDS             complement(14385..16187)
                     /locus_tag="VIG33_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY8270312.1"
                     /translation="MGKNLNKAALEKQKVLAFLDAEDFSVPSNPGIYGSKAEKGDFDS
                     LFEASVKDQDFNLGDVVTGKVVEVQSDYVLVDINYKSEGLIPINEFRVVDGVRQVKAG
                     DTVEVLIDRIENENGMVVLSKDKADMLRAWSDISKAAENEEVIEGTVVAKVKGGLSVD
                     IGVKAFLPGSQIDLRPVRNMDVYIGKKYKFKVIKFNKKRGNIVLSRRALLEEERDSLR
                     AETLDTMKEGSIVTGLVKNITDYGAFIDLGGMDGLLHITDMSWGRVKHPSELLKVGDE
                     IQVKVLKYDQEKERVSLGMKQLHPDPWESVKASYPPGTKLKGKVVSLAEYGAFVELGE
                     GIEGLIHVSEMSWTKRVKHPSQVVAVDQEVEVVVLEVDTENRRISLGMKQLQANPWVE
                     LKESYAPGTIIEGEVKSVTDFGIFIGIEEGIDGLVHISDFSWTKRVNHPNELYAKGQK
                     VRAVVLGVDIDNERFSLGIKQLESDPWSNIENKYAIGTQHDVKVTKIADFGAFVELES
                     DIEGLIHISELTTDKINTTEDFVKPGTVLKAEVISIDKDARKIGLSSKLIKLRESKAD
                     VEDYVKKATATSKSTLGDLFADQLKNMQKTEGKS"
     gene            16346..17665
                     /locus_tag="VIG33_05450"
     CDS             16346..17665
                     /locus_tag="VIG33_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270313.1"
                     /translation="MKTPGKFQGDSQGSGKHPLMQYFTQKLIFFMACLLIPLASLARD
                     FHPEILSALRYLEDSQVRSQGVYEIGEWPTQVTAVLLPSLIGAGKWGKPYQEPTIFTT
                     ATIVNLLNSIYKDSPELSTIPPMIERAIDGFGGFSEGPFFNFYPLKNYHGTWVRGPRS
                     MPLAPYLQGLAHIPPDADTTSVTYLALQAPVPEKVISAFSRFRDQNRKSHHYNRLYSN
                     INTGAFMTWLMDEKDPAMPRGTGHPELGPRIPFGTNDVDCIINANILKLLTAQKRTEV
                     PGYLEACTFLKESIRYQHYNTCGIYYPNDYILPFRVGELKALGARCLSGKNSTVLQFI
                     LDTQNLDGSWNSTPPNRPDPINSTALALNSLLLLGNLENRDHRVRVHNAVNFLLENGH
                     RDRQGNLYWNGQVFFSAIAEVRYSVIWRSNSYTTALVARALQLAENF"
     gene            17684..18019
                     /locus_tag="VIG33_05455"
     CDS             17684..18019
                     /locus_tag="VIG33_05455"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY8270314.1"
                     /translation="MMSILIHWFVSALVLMGTAYLLPGFEVRSFGSALLAAVIIGFAN
                     ILVKPILVLLTLPITILTLGLFLWVINALILKMCAALTPGFVVSSWGSAFLGALIIAV
                     LSALTSWLF"
     gene            complement(18068..18499)
                     /locus_tag="VIG33_05460"
     CDS             complement(18068..18499)
                     /locus_tag="VIG33_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270315.1"
                     /translation="MKSLMSTIFTVLFATSAFAGEGKTLKSLDAEQLLNALTRADAYV
                     ACTTLPCSTVASEISCSTMKASGPDCTLNVQSDEKGTMVHNQVTGERAQELAIALKSA
                     GIMNCGPKGCTGSAGTIQCTLPGLETEEDSSTQCAIDGAAF"
BASE COUNT         5370 a         4484 c         3650 g         5267 t
ORIGIN      
        1 ataccgctcc tgccgaaagg aaggtgccgt taaattgacc cgaattccac aactgcaaat
       61 gattttttaa accactttcg ataaaaaagc cagcattctc aaagacgtca aagaaacgag
      121 cgcctgcaat taattgatag tccagagcca gagaggcctc tccatcgctt tgtcgaaaaa
      181 aaacgccagg ccccatgcca aatccaaagt acaaaggaaa ttttgctgcg gcatcgggga
      241 atgtaatcaa aggcaaaata gacattttca aaggcttagg ctcgccgttt ccagtcacat
      301 tgaattgtat aaaatcaatg cgaatgttta agtccatgga gttcttccac tcccccactc
      361 gataggtcaa acccgtggtg aaatcgccga cattggtttg cttactctcg gtcccccatt
      421 cgtaggccgt cgagttcatc atcttcccaa aatgcaaagc cagataatga tcactaggac
      481 ccgaactcga gtaagaactg ggcttcgaac ttgaatccgc ctcttcccct tgttcacccg
      541 catcagaatt ctgtttttca aaatacttgg aggccacctc tcgaccgaca cgtggagctg
      601 gattcgctcg agcaaaatca tgagaaccca ataaaacaat caaaattggc agaagtagtt
      661 tcataaatct atgatacgca aagaggcact gcaatgaaag caaatgactc ttgctgtcat
      721 tatatggttt ttattaaggc cttgattgac ggccctttca gggctttcgt actttacaga
      781 tcaataaaaa agaacgagac tttagactat gaaatgtcct tattgcaacc acagcgaaga
      841 ccgagtctta gatactcggg tccaaaaaga cggttctatc cggcgacgtc gtgaatgcct
      901 cgagtgcaaa gttcgtttct caaccttaga aactttaatg ttgagcttcc cctttgttat
      961 caaaaaagac ggacgccgtg agcccttcag taaagaaaaa attcttaaag gtcttcaggc
     1021 gtcttgtcaa aaacgtcctg tcagtctggc tcaacttgaa actattgtcg aacgcattgc
     1081 ctcttggctt attagtcggg gagacaatga aatccccgct cgacttttag gtctacgggt
     1141 gatggcggaa ctcaaacaac tggatgatgt cgcttacatt cgatttgcaa gtgtctatcg
     1201 tactttcaaa gatgtccaag aatttgttga aacacttgag gacatggaac tggcagaagt
     1261 tattaatacg aataatccgc aattaccttt aaccactgtt aactattctg gaaagcctga
     1321 agaaaatgag aagacaatcc cgggaactag cactacaagt cctctttcaa attgagttcg
     1381 ctccacaaat ttccaatgct gatctaatgg aggtcctagg ggcttccctg gaacaggaca
     1441 ccgttaaatt tgctgatggg cttattcgtg gagtgcaggt gcaaaaaacc gcaatcgacg
     1501 ctaaaattca agcagtcagt actcattgga aactggaacg tatggccact attgaccgta
     1561 atatcctgcg tgtcgcggtt tatgaacttt gttttacgcc caatcctctc aaagaaaata
     1621 ttatcattaa cgaagctgtt gagattgcca aaaaattcgg tacaacagaa tccgcaagct
     1681 ttgtaaatgg tcttcttgat cagatcgcaa aggctcaaag atgaatgttt tgtccttggc
     1741 gagtgctttt aatcccggct ctgatctctg gatcattccc gatcccaata actgccgctg
     1801 gactcaaaag atcgattggt atttgaactt tcaaatcgtc aaaagccaaa gacacctcag
     1861 ccctgagacc cgcaacttta caaagctcat tcaaaaagag actggactgg aaatctatga
     1921 acacaccctt atgaaaaatg cacctctgat gatgacatct gaggccttct ttccaaataa
     1981 atgggtgatt gtagtgcccc tgtcggcaaa cttttccgct tgggtaaggg aagtttccaa
     2041 tatctgggaa aatctaaagc acccttctct gagggttttc ttgccgactg gacaaaatgc
     2101 caggtccttc aaccaagaat ggcaaaatca tcatagcttc gaagatttca cactggtact
     2161 tgattaatcg cctcaaaatt gaggaaataa tcatatgaat attatcactc atttccaaaa
     2221 acgtattgaa tccattctcg ataatgaact ggatgcccct agcgaagagg ttctcttagc
     2281 aaagaagcat ttcgaagctt tgacggatcc tcgcttacta cgaggcctcg ctgaaagcct
     2341 tccccaagat agcacggata aagccatcat tcttttttct cgtctcgcgc tttattttga
     2401 tgccggtgta tttgtagaag ctgataaaaa tctttgggaa cctcaagctc actttcatcg
     2461 tggccacatg agtgttctta aaactccgca aaaaaaatcg atctcgcttc cgaaagtgga
     2521 tttgatgaaa gtgctgcgca cggacacgaa acctattttg caaaaactgc atttggaaaa
     2581 attggacacc gaaaataaaa ccgtttgctt gttgttgaga ccgggagttg attttacttt
     2641 tttacttttt tcttctttgc ccgatctttg gttaaaagaa cacatgcgcc tcgtcaccga
     2701 ggccattcac catggaatcc ataactaatg acgacttgga cgatttatat tctttctgac
     2761 ggaaccggtg aaaccgccgc aaccatgatc cgtgctgcat tagtccagta cgccaataag
     2821 gatatcaaca ttgtccgctg taaaaacgtg cgcactgaag atcaagtcga agccattatc
     2881 gaggaatgct tcgaacgccg aggctttatc gcctatacgg tcgcgggtcc aaatatgcgt
     2941 aagaaaatcc atgagcttgc taactccaaa ggccttcctt catttgattt gctaggacct
     3001 ttattaaata ctcttgataa cttctttggt gatgccaaca ccagtacggt gaatgtcctc
     3061 cgcaccgtcg atgatactta ttttaaacgc attgaagcca ttgaatacac agtcaagcac
     3121 gacgatggga aaacattaag ccacttggac aaagccgata ttgtgttagt gggcatcagc
     3181 cgcaccagca aaacacccct ttccattttt ctcagccata aaggctggaa agtcgccaat
     3241 gttcccatgg tgttgaacac gccacttcct gaggagctat tcaaagtcga tcaacgccgt
     3301 gtggttggat tgattattga tattgaaagt ctgcatcgaa ttcgcaaaaa ccgtcttgaa
     3361 aaattcggcc aagatactgg aggcgaatac gcttcgatct ctcatatcgt taaagaaatc
     3421 gaatacgcac accagatttt taaacagaat aaacgctggc cggtcttcaa tgtcacggag
     3481 cgagctttag aagagacggc tggagagatt gtcagaatta tctcagcgcg cttagggctt
     3541 ccggattcag tgttgttcta aaagcgcata cttgcgcaat agaataagca tcacgcaaaa
     3601 cctctgtata aatgagactg ccgcgcatag gccaaaagat ttgcatttgt tcggcgatgc
     3661 taagctcaca aaatcctgtt gagatatagc aaccgacatg actgtgaaaa gaggtggttt
     3721 taatctcacc acaggttcgg gtcacggaat catcagcctc tgggaattct tgcatcaaac
     3781 agacacggac tttacgcagc cagttccgag cttcggttga ataattctgt tctgaattca
     3841 aaaaaatctg gcagatttta aatccaaatc ccaaaggata tcctttggga ccgcatggat
     3901 ggttcttctc ttcacacaga taataactgc aggaatccac ttggggaata cacttcgcgg
     3961 caaattgccc agccaaagac aagacaggac ttaaaacaaa aagcattgtg attatgaact
     4021 tcatgagatc cacctgttta aaaagaacag ttggtggatt agcgatattc atgccatgac
     4081 aggagcctct attattaaac tgagaataaa aagcatgtaa gatgttcatc ctaaagcgct
     4141 cattgactaa aattgaatca gcctctccgt tttttactcc gtaaggaaaa ttatccgcta
     4201 gttaaataat tcgaagtagg tattaaaggc accctgatgc tccaccagct gagcacgaag
     4261 gccgcgtacc ttctgaagat agaccctaaa ttctttcttc ttctcggcac cgaggattcg
     4321 gtcaatatgg gcaaagaacc gcaaagctaa ggcacgacgc tgttcgatat tactctggtc
     4381 cgcctttgtt tttatagatt ccactgcaaa atgaacgcgc aggccatagt gatccgataa
     4441 gggtttgcga ggcgttcctc ctagattgat acttccatca aaaggtttca attgagtcgc
     4501 atcgccaccg atattagaga aaaaaatata atctaagaca tgatctccag ccagccaccc
     4561 taaaggattt gactcacagt aggtgcaaac accttttgga taggtgccac ccaaggtctc
     4621 caccagagaa tcgcgcacgc ccagtagtaa cattataacc gccctttcca gagaattctc
     4681 atcaccgtta aagtcgccgg acaaaagcag cttcaaatca gtatgcgcca atctccaatc
     4741 caataaatcc aagatttgcg tcaagcgcac cgcggagttc gtggggtgaa gatgggtgtt
     4801 cacaaaataa aagtcttcgt caatcccagt cagcgacgcc tttacaacat gataagcttt
     4861 cttcacattg aaagctccgc gaacaagatc taacacattc ccctcactgt tcactttgaa
     4921 gtcatgagtt tcggtgccct tgatatcggc catcatcaac gacataacgc cgatcttttc
     4981 taggcgattt ggcgcgctga tattgtaata tcttctgaga ctactttcga tcatatcgat
     5041 ctgtggtgaa ttccaaactt cttgaaggtg aacgatggtg caaataggtt ttgcgtacag
     5101 ctctgccatc agcccttgcg tgcgttcttc catatggaac gcgtataagg gaccgtaggc
     5161 attaaaattc tgcatacaga atcgcgggat cccgggatca gcaatccaga atctctcagc
     5221 cttggcggac accatggtga gcaaaaggct tgccattaaa atcatctgct taataaacat
     5281 aacacctacc atttttgtag gtgtacttcg tctatcccat taaatctgtc aaagtaaatt
     5341 ttatagagat gactaatatc tatcgtttta ggccttctct tgagtcgcga tggtctggta
     5401 aagaatctct gcgagctgac gagcctcaga agtcttacga aaataataag tcatatcgat
     5461 ttcgtaatgg aactccttca catgcacatg atgcaaacgt cccgagcgaa cttgcttacg
     5521 aatggagtgg gcaggtaaaa aaccccagcc caggccggat tcaatgaccc gtttcaaagt
     5581 ccccacattt gaagactcaa aaatcggttt cggattgatt ccaaccccac tcatgagctt
     5641 ctgtaatttc gcattaaatc caggaaattc atcaacaaaa ttcactagtg ggaaatggct
     5701 taactccttg gccgcaattt gcgaaggcat atctgtttct ttccccgaaa ccaccaacca
     5761 catctcttct ttcaaaagaa atttttcttc aatattatcc agctccaggc caaactcttc
     5821 ttgcagttgt ggtaaaatta aaacgtcaaa atttcctttt ttaaaaccct ttaccaaatc
     5881 ttcgccagtc ttgtactcca ctttaatttg caaatcagga ttatggcgca tgaggcgtcc
     5941 aatcagcgga ctcataatat gcagaccaat agaatttaaa gtgccgatac gaatgagacc
     6001 tttgagttcc gctccaatag atttaatagc gatctcagtc tgttgcgcta actgaagaat
     6061 tttacgggct tgctcataga gaacttcccc ctgtggactg gctttgattt gtcgaacacc
     6121 ccgaaccaaa agttccacac ctagatcttc ctctaggttc cgaatctgct ggctgaccgc
     6181 tggctgagtc aaataaagct tatctgccgc agccgtcata ctgccttcac taattacagt
     6241 tacaaaagtt gtcagctgat aaaggctcat catgggattt tctcctttga tcagggaagt
     6301 ttattaacta tacttatgtt gttacaacca tcaatgaggt cctaaacatg aagtaccaat
     6361 ttttcaaata tcctctgatt actttattat ttatcttact cacaagtgcg gcccacgcca
     6421 aagtatttcg taatgcctat atctcctttg agcttcctcc cagttggaat tgcagcctcg
     6481 agcaaaccga gtgggtctgt cgcagtgaaa gctctaaaga agcaaaagaa gcgatcatca
     6541 tcttaaccgc taaagaagtg ggacctacgg attccacaca gctttatgaa tcccatttga
     6601 atagtcctat tacactgaac ttgaaaacgg gtgggacttc cgaatctaaa gtggtttata
     6661 aagctaaaac ggtccagatc aatgatcaaa actggattga tggtcttcat atgggaagcg
     6721 aagttaccaa ctactttact cggtatctgg caacaatcaa aggtcgcatc gccgttcttg
     6781 tcacgtttag cgcccacaag cagttctaca ccaaatatag ccaggacttc tttaaggcag
     6841 tgatgagcct acgcgtgatt gaaactaaaa acctggctaa taatccagag attcgcccag
     6901 gagctggcac cttaggtcct ggcagtttcc aaaattcaat gccagcagat atgagcaacc
     6961 ctgatgatat gcaaaccaat agcggcaaag gatctaaaat gatgctgttg gggattgctt
     7021 tgatcttagc cgcagccgga gtttacctct tccttaagaa gaaacgtaaa aaatagtccc
     7081 ccgcctctca tgttctggcc tacttcagtt tttcaaggat cggtttataa tccttcaact
     7141 gatccgccac tttcttttca tctgcataga ccagaacgcg caggtttttc ccgtccaaat
     7201 gtttttgaat tgccttattc acttcactca acgacagggc ttcaacattt tttgtgaaat
     7261 cctgcaaata acttagaggt actccataga aattcaaaac cagtatgtta taagctaaac
     7321 ggtccgccgt ttcgatcgct cggggaaatt gtcctaacat ttgctcttta gaagccttca
     7381 gttcggcttc cgtgatgcct ttgtccacaa attcagacaa aactttcaga ctttcactta
     7441 caacctttcc aaccgtctca ttctttgtaa aagtactaat caaaaaaggc cctcggtccg
     7501 cacgtgaatc aaaagaggag taaattgaat acgtcagtcc caagtcatca cgaatgtgtt
     7561 gattcaagcg gcttccgaat tctccgccta aagccacatt ggcgagacgc aactttaaaa
     7621 agtcaggatc attgcgacga atcccaaatt cacccatgcg aatttgagcc tgttgtagcc
     7681 ccggcttgct tacaagcttc agctctagac tctgaatcgg ctttagttcc ggagtggcaa
     7741 actccttaat gggttttcca gaccactttt taaaagcctc agaaactttt acttcaaagt
     7801 ccttattgaa gcgtcccacg accgccagct ccgcattatt aggccggtag ttattcaaaa
     7861 aatgacgaat gatatcttgc ttagttattc ttttcacaga ccctggcgtg ccagtgatgt
     7921 cattcgcata agggtgcccc tgataaagaa actcatcaaa ggcatcattg gcataatttg
     7981 agggattatc aattttcttt tgctgttggg cgatgacttg agatttcgcg cgggccactt
     8041 ccacatccat gaaagctgga ttcattatga tatctgagta taaacttaaa agcttatccg
     8101 cggagttcac taaggcatcc atggtaatga tggtgaaatc gtttccggtt tctatcccta
     8161 tttctgtgcc taactgcccc atttcatcgg ctaaaaccat ggcattctta atctgaatcc
     8221 cctgttcaag aaactgaaca gttaaattat tgattccctc ttttcctttg atgtcctgac
     8281 gtgcccctac tttcacaagc atcgtcatac tcacccgtgg caaggaatta tcggcaataa
     8341 agataatctt aagaccattc tctaattgca tctcgcgcat aggacgaagt ttaaactgac
     8401 tttccattgg cataacgaca acgggcgttt tatttacttt cacaatttct gttttctttt
     8461 ccggggcaac gcaacctact aaagaaatca taataagatt tagcaagatc ttcatttgag
     8521 gtttcctcat cacttattcc ttcttccctt gagattctaa aacgacaatg gatctttgat
     8581 tttgatttaa atatttactc gcgctttctt taatctgcaa cgcggtcacc gcattgtact
     8641 tattgagatc tttaaagaga ttttcataag agccggtcat aatctcattc accgccaagg
     8701 cccgggcctt gccatcgata gtcgttaaac catccaccag ctctttcata acttgagtct
     8761 tcgcttttgc caactcttcg tcgctgacta atttattacg cagtttccag atttcttgat
     8821 aaaccagttc caagttctct tccattggca ttcctggctt caaattaatc ccgaccgcga
     8881 aaactccttg atcttgcatt gtggaattat aagcataggc ggaagtggct gattgtttag
     8941 tatagaccaa tcgtttatgg agacgactgg aagagccatt ccccaaaatg tttgcggcaa
     9001 ggtccaaagg atacatttcc gcgtgagcat tcggcacact gccataagcc actgtaaagg
     9061 aaattccttg aacgtctttt tttactttag cgttccgctg tgtaacttga acgacttcac
     9121 gaggatattc acgacgaggc aaaggtcggt aagggatggg gccgtaatat ttttctatga
     9181 gccttttagc ctctgcagtt ttaaaattcc cagctaaaac gaggatggca ttattgggaa
     9241 cgtaataagt actgtgaaaa tttctaagag tttcgatctt gtagtcagaa atatccttca
     9301 tagttccaat cacgggccaa cgatagttcg ctgtcttaaa aaccgtgttc atgacagttt
     9361 ccatcaagag gcccatgggg ttgttatcaa cgcgccatct ccgttcctct ttcaccacct
     9421 cgcgttcact ctgaagatct tcaggattca aattgaggtt gttcatgcga tccacttcaa
     9481 tttccatcat catctccaac tttgagctgg gcaggctctg gtagaaaccg gtgtaatcat
     9541 aagtggtgaa agcattatta acgatgccat tttcatgcaa aaggcgatcg aacgatttac
     9601 cgggataact cttcgatcct ttgaacatca tatgttccaa catatgagcc gatccggtaa
     9661 taccgggagc ttcatcacga gaacccactt tgtaccaagt atggtaagta atgagtggca
     9721 cggcatgatc ttcgtgaagt aaaacagtca ggccattttg caacttgtat ttggtcacct
     9781 gaaaattgat tttcaaattg tctttctctt ggccatgagc atgagaaatt ataaagagaa
     9841 tggaaaagaa aaaaagaagc tgcttcatag atagactcct aataatcatc ttattgtagg
     9901 aggtctctta ttttaaaggc aatatattta caaaattggc cttattgggt cgagttactg
     9961 aatgacgaaa tcagaaaaga aaacatcttt caccacgccc tcttggagga tggtatttac
    10021 ttccatcgcg atcgtatctt tcaagaaaag tttcccttga atgctctcga tctcggagaa
    10081 agttttatca ttcaacacac ggacgatttt atcgcgggcc ttggcccgac tgtcggggct
    10141 cataatctct tcataaccct ctttgcccag catctgcaaa ctcacttcaa gacggatgat
    10201 tcgcttaggc tcccctccca agttcaccgt gaatttatcc atggtaaaaa tataaggtgc
    10261 aaagcgctcg tcttcagact tggaggccat agtggtctcc aacatttctt cagtgatttt
    10321 cggactctgc caacctaagg ttgaggcata tacgagataa gcgcctccgc ccataattcc
    10381 catattcagt accgcaaaag ccaccgttaa aataaggccc aggtttttta ccttcgccgg
    10441 tggccgttct tgtttatcac ttgccataca atccccattt catataactt tatcggcttt
    10501 tgtgacctaa gtcttaataa ctttagacct gcttttgtct aaaaacttaa tatattttgt
    10561 cgaaccctta aacagtcccg cttcttcact tctcggtatt gacaagactg tattgacaag
    10621 acagcgcaga attctttcta atagagatat atgaccagaa aactctacac ttggattgtt
    10681 tcttatgttg ctgtgaccgc atcttggggt ctcgggggcc ttatcccttc attggcttta
    10741 gctgagaatg aagcggcctc gacatcccaa aataaaacaa cttctgaact cctcccttca
    10801 gatctcttac aaatttcaaa taccgatgca tttagcaaat atgttttttt agtcgataaa
    10861 acagaacgtc atttgcaagt ttttgaacga aaaggagaga ctatcgaaaa aattctcgat
    10921 gttccctctg atatgggaaa aaacgtcggc aataaaatca aacgggatga tgcgcgcaca
    10981 cctgaaggca tttatttttt tgaaaaaaag ctaaatcaac ctgaaattcc attcggtctc
    11041 tatggcgaac tcgccttcac tactgattat ccaaatattt ttgatcggcg tgaaagtaaa
    11101 accggatctg gaatttggct tcatgcggtt cccgatacgg ttcccttgac tcgtggatct
    11161 cggggctgcg tagtggttcg taatgaagtc atcagaaagc ttcaagaata cattaagctt
    11221 aaagaaactc cgattttgat cttcgataaa gtgaactatg ttcccaaaga agaacatgat
    11281 aagcgccgtt ccgcactgaa tgattttatc gaaggatgga gaaaagcctg gcagtcccaa
    11341 aatattgacc aatacatgac ttattatgat caaaatttca aggctccggg atttaactac
    11401 gctagctgga aggcccacaa gaacagactc aaaaatcact atgagttcat taaagtggaa
    11461 ctctctcagc cctttctgct tctacacaaa gaccagttga ttgtaaaaac tttccaaaga
    11521 tatgaatcca ataatcacgc ggactttgga gtaaagacgc tctatgcggt gaaaactgac
    11581 tcggggtata aaatcatccg tgaggaatgg gtgcctgtca gcgagaactt gaccacctcc
    11641 acggctctga gcacccaacc caccgcgccc aacaattagt taattctagt gctggctaaa
    11701 ctcttcttca tctgattcat ccgcttcatc ttgagaatca gcaagttcaa cattatgacc
    11761 catgtctgga agagttttaa gatgagcctc aagctcttct ttcgtaagtt gtccgttgtt
    11821 gatataccat tcggtcaatc gcttatcgaa cttcaggttt ttcattgctt tgtctaaaga
    11881 catgagtgag ctccttttaa gaatcactta tatataagaa attagttcca atatctaggt
    11941 ccattgtttg atttttttgg attttcatta tccactacca aacgcagatt acgccccttt
    12001 ttcttggtct ttttaaagtt cacatagttc ttccatcggc tcatcagcaa taaagccaca
    12061 taacctgagg ccaatccccc aagatgagcc aaataagcca cttctccacc gctaacgccc
    12121 gaagtaagca ttgaagccat ctggacgcct cccattaaca tcacaaaata acgggttttc
    12181 attggaaaaa gcataaaaaa gtaaacaatg cgctcaccga agagaattcc ttgagccaac
    12241 atcaagccaa aaatagctcc agaagccccg ataacaggta ccatcaagcc aatttgcgat
    12301 cccgtaactg ccgcatagct cgccattccc aagcaataga gtatggctgc ccccacccca
    12361 gtaaagaggt aataaaataa aaagtacttt ccaccccagc gcatttcaag ctccgcccca
    12421 aagaaccaga gcatcagcat attgaacaaa atatgagtta cttgcatgga atgcaggaac
    12481 atataagtga ataattccca aacatggaat tgaaaaatca cccgggaagg caccagattt
    12541 aaaaaactgg taaaaggaac tttaaagaat ccttcgacta aaatttggaa aataaaccaa
    12601 attgccacat tgataataac tagccacttc actacgggag ttaatggaac tgccatactc
    12661 atttgtgatc cacgattcac agggattctc ctacagactt ccttccaaaa taccctaaaa
    12721 gtccgtcata cgtctgctcc agactttcaa caacgacttt ggtctcggca ataaggggaa
    12781 aaaaattcaa gtctccggac caacgcggaa tcacatgata atgaatatgg tcagggattc
    12841 ccgcacccgc cacagcgcca tgatttaagc ccacattgac cccgccaggt tgataaatgt
    12901 cttccaaagc tttcatcgcc aatcgcaaag tatcttgcaa atcataatga gcttccttac
    12961 tgagtgccaa aagatttccc tgatgtttta aaggaagcac cagtaaatga ccactgttgt
    13021 aaggaaattt atttaaaaca atcatggaat actttgtttt ataaacacac agagtctcaa
    13081 atgaaggttc atttttagcg gcactacaga aaacacaggc ttcgggctta atcaacttac
    13141 gaacatattt aaaacgatcg ggacgaaaca gaacatcacg ttcaagaggc caaagatctt
    13201 tagagatttt caaaaatact tttggcatct taattgatga gaccttggag cccgatattt
    13261 tattcttttt tttaagagcc gtcttctttg ccaaaaaaac tcctattccc aaaatcccgg
    13321 cataataagc attggctgat ttaagtactg agctctaaaa acttttcgaa tggagctttc
    13381 catagactta tcacccatat ttttagaaaa aacgcgagca atttcatcga tggaattcag
    13441 atcatcatcc tcttgctgtt ctatcagatc ccaaagacct ggacgtttct taggaacttc
    13501 aaaaagctta taatcagagc ccagatgagc cgcttcggct gccgctttaa tggcatcctc
    13561 caaagttccc aatgaatcag caaaacccaa ctgcaccgct tgcttacctg tcatcacccg
    13621 cccatcggca tattggctca ctacttcagg gttcaactta cgaccgtcaa taacagcctg
    13681 tttgaactga ccatagacgt cgtcaatcag ctcttgaaac aaggcttttt catctgcacg
    13741 catggccctg tactcggcac ctgaatcttt aaatttccct gagttgattg aaaaacgaga
    13801 aacttttgcc cactcataaa gtttttcaag atttgcaaac tccatgatca ctccgatgga
    13861 gcccactaaa gctccgggag cgacaacaat tttatcgcaa gccactgcgg agtaataagc
    13921 accgctagca agaagacctg aggaggaaca aataattggt tttttagttt cttccttaac
    13981 agctttgatg gcggcataaa tctcttgaga aggccccacc gcgccacccg gtgaattgat
    14041 gttaatcaca atcgctttta ttttatcttc agacttatac ttcttaagct tcttcatgaa
    14101 ctttttgccg ttcataataa caccctcaag atccaactgc agaatgctat ttttagaaac
    14161 caaggctgat tcctcgtctt tttctccggc aaagctgctg acccacttca ccacgctgac
    14221 aaggccgacg atcacaagaa taacgataat aaaacgcaca aaaggatttt tcattggttc
    14281 tccattaaga ttcccaatct taacagccaa aaaaaaaggg agtctatgag actccctttt
    14341 ttgcgctttg cgccttagta aaatagccta gtcctgatta gggtttagct tttaccttct
    14401 gttttctgca tgtttttaag ctgatccgca aacaaatcac cgagcgtaga cttagaagtc
    14461 gcagttgctt tcttcacata gtcttcaacg tcagctttag attctctcaa cttgatcaat
    14521 ttggaagaaa gaccgatctt acgagcgtct ttatcaatag agatcacttc cgctttcaac
    14581 acagtccctg gtttaacaaa gtcttctgtt gtgttgatct tgtcagtcgt aagttcagag
    14641 atatgaatca acccttcaat atcagattcc aactcaacaa aagcaccaaa atcagcgatt
    14701 ttagttactt tcacgtcatg ctgggttcca atagcgtatt tgttctcgat gtttgaccaa
    14761 ggatcagact caagctgctt gatacccaaa gagaatctct cattgtcgat atcaacgccc
    14821 aaaacaaccg cgcgtacttt ttgcccttta gcataaagtt catttggatg atttacacgt
    14881 ttagtccaag agaagtcaga gatatgaaca agtccatcaa tgccttcttc gataccgatg
    14941 aagataccaa agtcagtcac agacttaact tcaccttcaa tgatagttcc aggagcataa
    15001 gactctttca actcaaccca tggatttgct tgaagctgtt tcatgcccaa gctaatacga
    15061 cggttttcag tgtccacttc aagaacgaca acttccactt cctgatcaac tgcaacaact
    15121 tgagatggat gttttacacg tttagtccaa gacatttcag aaacatggat aagaccttca
    15181 atgccttcac caagctctac gaatgcaccg tactcagcca aagacacaac ttttccttta
    15241 agcttagttc ctggaggata agaggctttc acagattccc aaggatcagg atgcaattgc
    15301 ttcatgccca aagacacgcg ctctttttct tgatcgtact taagaacttt aacttgaatt
    15361 tcatctccca ctttcaatag ctcagaaggg tgttttacac ggccccaaga catatcagtg
    15421 atgtgaagaa gtccatccat accgcccaag tcaataaacg caccgtaatc agtgatgttt
    15481 ttaaccagac cagtaacgat agaaccctct ttcatagtgt ccagagtttc tgctctgaga
    15541 ctgtcacgtt cctcttcaag aagagcacga cgagaaagaa cgatgttgcc acgtttttta
    15601 ttgaatttaa taactttgaa tttgtacttc ttgccgatgt acacgtccat attacgaaca
    15661 ggacgaagat caatttgtga accaggcaag aatgctttaa cgccgatatc aacgctcaag
    15721 ccgcctttaa ccttagcgac aactgttcct tcgataactt cttcgttctc tgcagcttta
    15781 gagatgtcgg accaagcgcg aagcatatcg gctttgtctt tagagagaac aaccatcccg
    15841 ttttcgtttt cgatacggtc aatcaagact tccaccgtgt cgccagcttt aacttgacgc
    15901 acaccatcta ccacgcggaa ttcattgata ggaatgaggc cttcagactt gtagttaatg
    15961 tcgacgagga catagtctga ctgaacttca acaactttac cggttactac atcaccaagg
    16021 ttgaagtctt ggtcttttac agacgcttcg aaaagtgaat cgaaatcgcc tttttcagct
    16081 ttgcttccat aaataccggg atttgatgga actgagaaat cttctgcgtc caagaaagcg
    16141 agaactttct gcttttcaag agcggctttg tttaagtttt tacccatagt aataatatat
    16201 acctcccacg tgataggtcc tctagatggc tagccctggg ttattaaagg tcttggccgg
    16261 gtcctacccg tttaaaaaat tatgaagcat ggttgtatcg gccccttttc actgagtcaa
    16321 tgtggaaagt taaccccgcg ttgttatgaa gactccagga aaattccaag gggattcaca
    16381 aggctcaggt aaacaccctc ttatgcaata ctttactcaa aaactgattt tctttatggc
    16441 gtgcctctta atccccttgg ccagcttggc ccgggacttt catccagaaa tcctctctgc
    16501 ccttaggtac ttagaggact cccaggttag atcccagggt gtttatgaga tcggagaatg
    16561 gcccacccaa gtcaccgctg ttctcctacc cagtcttatc ggagccggaa agtgggggaa
    16621 gccctatcag gaacccacta tttttaccac ggccacgatt gtgaacctgc taaatagtat
    16681 ttacaaagac tctcctgaac ttagcactat cccaccgatg atagagcgag ccattgatgg
    16741 ttttggaggc ttctctgagg ggcctttttt caacttttac cctctcaaaa attatcacgg
    16801 cacctgggtg cgcggaccgc gaagcatgcc actggctcct tacctgcaag ggttggctca
    16861 tattccgccc gatgcggata ccacctcggt cacctacctg gcgttgcaag ctcccgttcc
    16921 agaaaaagtg atctcggcct tctcgagatt tcgcgaccag aatcgcaaaa gtcaccatta
    16981 caatcgtctc tatagcaaca tcaacacggg ggcattcatg acttggctca tggatgaaaa
    17041 ggatcccgcg atgccaagag gaacgggcca tcctgaactc ggcccacgga ttccctttgg
    17101 caccaacgac gtggattgca tcatcaatgc caacatctta aaacttctta ccgcccaaaa
    17161 gagaacagaa gtgcctggct atcttgaagc ttgtactttc ttaaaggagt ccatccgcta
    17221 tcagcattac aatacctgtg ggatttacta tcctaacgat tacattttac cctttagagt
    17281 cggcgaactc aaagccctcg gcgcccgctg cctttccggg aaaaatagca cagttctcca
    17341 atttatcctc gacacgcaaa acctcgacgg ttcttggaat agcactccgc ccaatcgccc
    17401 ggatcctatc aactccacag cattggcgct gaattctctt ttgcttctgg gaaatctcga
    17461 aaaccgcgac caccgcgtgc gcgttcacaa tgctgtaaat tttctcttag aaaatggaca
    17521 ccgtgaccgc cagggaaatc tttattggaa tggacaagtt ttcttttcag cgatagctga
    17581 agtccgttat tcagtgatat ggagatcgaa ctcttacacc accgctttag ttgcacgggc
    17641 gttgcaattg gcggagaact tttaaaaaac agggaggtat tttatgatgt cgattcttat
    17701 acattggttt gtttccgctt tggttctaat gggaacggct tacttgctcc ctggttttga
    17761 agttcgcagt tttggcagcg ccctgctggc cgccgtgatc atcggctttg ccaatatcct
    17821 cgtgaaaccg attttagtct tactcaccct gcccatcact attttaactc tgggactatt
    17881 tctctgggtg atcaacgcct tgatcttgaa aatgtgcgca gccctcactc ctggattcgt
    17941 ggtgtcgtcc tggggcagtg cttttttagg ggctttgatt atcgcggttc tgagtgcgct
    18001 gacttcttgg ttgttttgaa gtcagggatt tttgccctaa ctttttttca taaaaaaata
    18061 aatttaatta aaatgcggct ccatcaattg cacactgagt tgacgaatcc tcttcagttt
    18121 cgagtcccgg taaagtgcac tggatggtac cggcggaacc agtgcatcct ttcggcccgc
    18181 agttcataat tccggcgctc ttcagagcga tagccagctc ctgagctcgc tcgccggtca
    18241 cttgattatg aaccatggta cctttctcat cgctttggac attgagagtg cagtcagggc
    18301 ctgaagcctt cattgtcgag cacgaaatct cggaagccac cgtactacaa ggcaatgtgg
    18361 tgcaagcgac ataggcatcc gcccgagtca gagcattgag caattgttcg gcatcaaggc
    18421 ttttgagtgt cttaccctcg cccgcaaatg cactcgtcgc aaacaaaact gtaaagattg
    18481 tagacatcag tgatttcacg atttctcctt tttggcaatc agcgtttata acttaaaatg
    18541 acagcaaagc gtgccaatcc cccaatgaag ctgaattgcg gatgagctgg caaagtatta
    18601 ggtagagccc ataattgttt ctttaaaatt acgcctctcc gttttttagc ttatctttac
    18661 ccacaattaa aagcttcaat tgacctccat gttttttccg attgcatcag caccatccat
    18721 cctctccaaa cagagatcat tcctggtgga ccgtcattat ttcgtcctat a
//
LOCUS       DATBTE010000146        17846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1385576, whole genome shotgun sequence.
ACCESSION   DATBTE010000146 DATBTE010000000
VERSION     DATBTE010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 17846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17846
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385576"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..717)
                     /locus_tag="VIG33_05465"
     CDS             complement(187..717)
                     /locus_tag="VIG33_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270316.1"
                     /translation="MMTQKQAADFLSQSLHNWVEQYFKSFPGLIGVLQFGSTIKAPLK
                     HETDLDLLLVFETLPNSRWDQFQLTANLENKLNADLKKIDGFHIEVSFILKQESQLDH
                     LSSFYLDFIDTSKIWFDPKGVLNQLLKDINNWISENGSYKVKKGNLWYWVYSSKPGSH
                     TTVSFKFSKKSIDSKG"
     gene            complement(714..1127)
                     /locus_tag="VIG33_05470"
     CDS             complement(714..1127)
                     /locus_tag="VIG33_05470"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEY8270317.1"
                     /translation="MTTNDLAQDYLKRCQMRFKALKVLLDEGAYPDVVRESQEIVELL
                     LKGYLRLNLIDPPKWHDVGSILTDNKNLLSESIRANLDEITEFSKYLRRERENAFYGE
                     DDLIPLEHYDFKMAQTCYNKVEWMIYLFKPEFAKI"
     gene            complement(1233..1898)
                     /locus_tag="VIG33_05475"
     CDS             complement(1233..1898)
                     /locus_tag="VIG33_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HEY8270318.1"
                     /translation="MKSLNIIQESSDWLVIDKPAGISVHNDKVNVLSVLQQQKKNPNL
                     TFHAVHRLDSETSGVFMIAKDPEVAAKLMEEMQKDSTHKLYQAILRGPMKEETALWDW
                     KLSDKAEGRKNPQGLSKDRKECETLVKVLQKNEYLTLVECEILTAARQHQIRKHAAVA
                     EHPVVGDKRYNDQKYNERIIKMYGTERLFLHATELRIRLNGRDMVFTSPLPGEFRNLM
                     RGK"
     gene            complement(1898..3271)
                     /gene="mgtE"
                     /locus_tag="VIG33_05480"
     CDS             complement(1898..3271)
                     /gene="mgtE"
                     /locus_tag="VIG33_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEY8270319.1"
                     /translation="MNSENKNPSGSADSKEFSQAFSQTEEQFYDLFENWSALPTGERR
                     KKFKELPRTEAEELFLSLKTHDQVELLEEASPLEKRSWVRLLAPDDVADLIQEMGVEY
                     REEVLGLLDPQTKREVIALLAYAEDNAGGLMSSRFIRLRPDMSVDEAISYIRIQAKTH
                     VETIYYAYVLAADQTLLGVVSFRELFASSPDKKIKEIMTTDMVKVLVDTDQEQIAKVF
                     SSQDLMAIPVVDGNNHMVGIVTFDDVAHVLQEEATEDIHKLGAVEALDAPYLKISLLE
                     MIKKRAGWLMVLFLGEMFTATAMGYFQSEIERAVVLALFIPLIISSGGNSGSQASTLI
                     IRAMALGEVRLRDWWRVLGRELLTGLALGTCLSLVGLTRILLWPAREQLYGPHYVLVA
                     FAVAVSIVGVVLWGTLSGSMLPFLLRRMGFDPASASAPAVATLVDVTGLIIYFTVASM
                     FLRGVLL"
     gene            complement(3406..5325)
                     /locus_tag="VIG33_05485"
     CDS             complement(3406..5325)
                     /locus_tag="VIG33_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY8270320.1"
                     /translation="MSKPKYFSDGEQQKQESGYSRAVLQTLGEAYRPFKNKIIFTFFA
                     GLGGRTLLLANANIIGVWVDSFCKPQQGIQCKPLPSFFAEFSSEKYLQLLGVMTLIGF
                     VLTLCFRVIFSRLSAEAISQIYDEVTLRTSRLPMSFFDATPAGRIITRFSSDYGNVFR
                     LFGGPLAEFCSIIFDLLVMIVLITIASPYYLAFVVFVALLNYGVYYLNRKKLRQSRRD
                     LSASRSPSIAHFAETTQGASTIRSFRRQESFMDRFSRLDRYFLQQKLKTTGRVIGFSI
                     QMNSLSALLLLTTGVSAYYMVHQGLASVGSVGVAFSFIALSANTVQMFFEWLTQFEEA
                     MIGVERLDQYLRKDLEPGSLLPSSAHFNTGHPKYDETLEKYLHNRRLTQERNASVQVQ
                     SLWFKYREDLPWVLKDVNFEVKAGERLGIVGRTGSGKSSLIQALFYLYPLQGGRIAIN
                     GHSPKINIPKIKNSDKGLAKGSEAGMDLNLYRRSIAFISQDPVLFQGSLRSNLDIENQ
                     LSEERLLAVLDQVGLKEWVTQQALGLNMRIEERGKNLSLGERQLLCMARCLLQEAPIV
                     IMDEATSSVDPQSEEILVKATEEFFADRTQIIIAHRLSTLEKCDRVLWLQNGEIHSLG
                     PTSEVLPRFEAAHLS"
     gene            complement(5312..7096)
                     /locus_tag="VIG33_05490"
     CDS             complement(5312..7096)
                     /locus_tag="VIG33_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEY8270321.1"
                     /translation="MKPLIFDVLFNRFHARVLIVLSSLVATIVALFGPYFQKLFIDNL
                     TGHLSAQASLLPVGFQGSPLIFILLAFFCILLSQAFFQLTNYLGMRESIHLQSVFSHR
                     LYQKTLDLRVDTMSSRPVGEIIQLYATDIPGSTVFLEQTLPAGASTLFPLILAPFAIS
                     LMYDLPMLPTLLLMMVITGLNTWMAFRQSRFFYRFKTLAGERIALVNEWIQNIRTIRI
                     LGWLGLFEKNIFQKREVETANRVIMVTNGQIMNSISTSITFALNVVTLVSLIYYSKRQ
                     VTSGELLALLWIVGVFLTRPFRQMPWFFTFAFDSWTSLQRLESFLRITNENDGHESAE
                     DHSKEPFKAKENLALEVKNLNLQISGKQILKNIHLKIREGELVAIVGEVGAGKSMLLL
                     SLLGETGAAADVYTLQGKSVADMTLNDLRAHFAYVPQEGFIMSASLRENVAFLYDIEI
                     ERDSDIEESLRLAQFEVFKERVENGLATEIGERGVNLSGGQKQRVSLARVHFHKAPTL
                     LLDDCLSAVDVDTEEKLFTQLIDGAWAERTRLLVTHRLSILHRMDRILFMQEGSIVDS
                     GTYAELMDRSPLFRDYTQSVVEAKHVEA"
     gene            7095..8798
                     /locus_tag="VIG33_05495"
     CDS             7095..8798
                     /locus_tag="VIG33_05495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8270322.1"
                     /translation="MVCDLTSYPLRVKGLCDSTVKIFFPLLPSQIWIQNKQMARSLDF
                     KKIMGILQEESEEFSSDRRAFLKKMVYSPLSVPMASLALAGCSSFDSWVIGDNKHLDQ
                     EVMVLGGGLAGLSAAYHLKKNKIPYKVYEASSRVGGRIQTLFHANEDEQFAELGAEFF
                     EASHELVIQLCKELNLSTQDISYDPKLNRSLYCLNGKVVNEKDFRKNLKPLALKLAQL
                     RRESFASFTSEINPRALAINPSMVALDQMSLFDFVNPLHGTMDEATLQSFENLCISEW
                     GADLKNINLLHFLVKLDVEERSSAASPQKIFRVQGGMARIVQTLGERVQGIVPNLTLK
                     LEHQLIAIREKSGGYECTFKTTKGSDTVWARQMICTLPFSLLKEVDGIQSLDLGRRKD
                     FIANSNYGTHAKVICSFKDSFWKKKQNATLASQGVFRGQLAGQSYWDSSRGQAGTHGL
                     LTSQRGGSVGQSTGASAAQQIREDLQCFYKEPLAEENFHLSNWSQKPFAKGSRLNLLP
                     GTYLKYLEILLEEKELEGFYIGGEHWSFAHSGTMNGAIETGVAAAEKALKKAFFNGVF
                     K"
     gene            8888..10069
                     /locus_tag="VIG33_05500"
     CDS             8888..10069
                     /locus_tag="VIG33_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270323.1"
                     /translation="MILRLPLLVFVLALGGFFIPEASNAYPDFISYGYNTCITCHYNG
                     QGNGPLNDYGRALFTTEIAARDVFNKGLSEEEIAAKSGFLGSTELPWWIRPGLKYRGL
                     YFINNPGSEAAVKKYVIMQTDLNVAVQFDPKAKYLFIASGGYSPTPASQQANKDPKDK
                     NFISREHYFRWQANKKFFSYFGLMDKVYGVRTVDHTAFSRVVTGIAQNDQAHGVIGQY
                     YGGSWELTGNIFLGNLLQEEANVRQQGASIMAEYDMAEKNRVGTSFLFSKNDYLDKTR
                     FELHSKLGLDKGNSLLTEIGFVKDNPKLSPSNKYGGYLMMEGVYLITRGYNIISQIEY
                     YNATLSPESPDMTRWTFGLLMFPMPRMEFRTTFVNGRSITDTTVTNDLWMLQAQLHIS
                     L"
     gene            10078..10677
                     /locus_tag="VIG33_05505"
     CDS             10078..10677
                     /locus_tag="VIG33_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270324.1"
                     /translation="MKKMMSAIFVTLMVSPALAQTSQKNYLSTEAVERYLLTLDYNSW
                     FEKLTITNSSGEEQKSQSLYYGFGVGLEKNWYQPNWGWGLGTGVLAGSAVGGDKSGTL
                     TYFQARVPWWGVRLTPRLFYRWNPRTDFGLDLSSFYKQGNWPAGTNNLTAKSGADLIS
                     GAFIDMRIRFNQKIEMMQSFGVIYKDESIYWRIGLAYRL"
     gene            complement(10687..11070)
                     /locus_tag="VIG33_05510"
     CDS             complement(10687..11070)
                     /locus_tag="VIG33_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270325.1"
                     /translation="MKRALRAGLTIFGLLAPLACGKIYNSSTYDATTYGASDGSVKFL
                     AAKEIIKTNCATCHTRASHQAWAGMNEQDFISQGLVVAGNFSGSLLYTKIQGNRTSTP
                     GNMPEGGTLLSGAELTVIEDWILGI"
     gene            complement(11105..11596)
                     /locus_tag="VIG33_05515"
     CDS             complement(11105..11596)
                     /locus_tag="VIG33_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY8270326.1"
                     /translation="MKMAALFLPMPVLLFSTIAWSASVTVDVNLSPAGSFKGKTSDIK
                     GFVTKKGDEVSARNIVVNLKNFKTGIELRDKHTLKHLETDKYPEAILISATGKKGKGA
                     GKIRIRGIEKEITGTYKINNNELLADFKLNLADFKITNVKYMGAGVDDEVVLHIAVPI
                     KGP"
     gene            11765..12448
                     /gene="folE"
                     /locus_tag="VIG33_05520"
     CDS             11765..12448
                     /gene="folE"
                     /locus_tag="VIG33_05520"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEY8270327.1"
                     /translation="MKKITKKSKTSLYHHFSAQDILSHVLPTPMVKNGLSEEEKIEKI
                     TEKFSEIMEVLGLDLSDDSLRDTPRRVAKMYVKEIFRGLNPEHFPKMTVIENKMKYDQ
                     MICVSGVSTLSFCEHHFLTIDGVATVAYIPNKKVIGLSKINRIVQFFSRRPQVQERLT
                     KQIADCLQYVLETEHVAVYIDAKHYCVITRGIEDTASSTVTTEVRGHFRTKVQTREEF
                     LLQCKRIHR"
     gene            complement(12462..15098)
                     /gene="pepN"
                     /locus_tag="VIG33_05525"
     CDS             complement(12462..15098)
                     /gene="pepN"
                     /locus_tag="VIG33_05525"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEY8270328.1"
                     /translation="MTKQGNKIYLKDYTKPLFEVGTLALNFNLNEDFCRVLATSQIKS
                     LKAGTPLFLEGVDLNLLSVSINNEKLSSSQYKLTSEGLEIANVPTAFELKVETEIYPQ
                     KNTSLEGLYRSNETFCTQCEAQGFRKITYFMDRPDVMTRYTVTIEADKKKYPVLLSNG
                     DRVKVEDLGNGRHKAFWNDPHKKPCYLFALVAGDLGVIRDHFITKSGRKVNLEVYAAH
                     GKQDRCWHAMESLKKSMKWDEITFGLEYDLNDYMIVAIDDFNAGAMENKGLNIFNSRL
                     VLADSHSATDADFHAIESVVAHEYFHNWTGNRVTLCNWFQLSLKEGLTVFRDQEFSAD
                     MTSRSVQRILDVDSLRERQFTEDAGPNSHPVRPESCMAVDNFFTATIYEKGAELIRMM
                     QTIVGQKGFRKGMDEYFRRHDGQAVATEDFAAAIAEPNHQDFTQFKRWYSQAGTPQVI
                     VSENYDAAAREYHLTLAQSYPSSVGHPETQAEKKPFHIPLLMGLLNSQGKEMELHCNK
                     ITRNSDGKTLIELREGVETFVFKLVPERPVLSLLREFSAPIHLQWERPLEDLYFLMEK
                     DNDEFNRREAAQKIILRLFADLIAQHQAKQTLTVNNKFITAIGTILHDENLSEAFKAQ
                     MLQLPSDILLAQEQPILDAPAFFAARKKLRHEIALAHKKSFADIYGKFHNVEPLSRDP
                     KVFGHRFLKNIALSFLQELDDATLTEMVVQQYQTAQNMTDQLVALELLASGGSNYRPK
                     ALEDFHQKWHGDSVVMNKWFIAQAFSSRKDTFETVKSLTQHPSFNINNPNNVYALLRS
                     FTQNFVIFHNPEQPAYEFLTDMILKIDERNPQVAARLCSAFNFVNKFPPQMKKQALQQ
                     IHRMVAHDKLSKNSRELLQNSL"
     gene            15188..15556
                     /locus_tag="VIG33_05530"
     CDS             15188..15556
                     /locus_tag="VIG33_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8270329.1"
                     /translation="MGIVFTSITVNTPNLENMIRFYEILGCEFSKVKVNIGGELFRSC
                     FEGFELSLMSIKSAPREGMPKVMMGFQVSDFDSKVKLLALVPGVLLILDPTDMPDGRK
                     AIVQDPDGHSIELLSKEMLL"
     gene            complement(15598..15984)
                     /locus_tag="VIG33_05535"
     CDS             complement(15598..15984)
                     /locus_tag="VIG33_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270330.1"
                     /translation="MKNKTFISISFFCNFAYATSEKFIYEKMERDQKRNYFAIQETRI
                     SDTEECRKKTCLAYEISVGKIKVPAEIEWIPGGVNPTSTLCKKMEGTPKIGYMENRNE
                     VSLCYFNDHIFIFGWDLMKISRKSNR"
     gene            complement(15974..16444)
                     /locus_tag="VIG33_05540"
     CDS             complement(15974..16444)
                     /locus_tag="VIG33_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270331.1"
                     /translation="MIQSLKEKVPEQLLMGKDLRSLFNNSSSESDFLKNTLVSSACEK
                     NRNQVSASIQSKTAPKSNSEYEQRQMADFIADGLKSNSSVGIVMDAGLTGMYRYPNNS
                     IHGLVITGMRYRAWSHTCEFQLRNSYGEGGDFNEWIPVDILQKAILSARYLSEK"
     gene            complement(16441..16950)
                     /locus_tag="VIG33_05545"
     CDS             complement(16441..16950)
                     /locus_tag="VIG33_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEY8270332.1"
                     /translation="MVTLIRFLVALTFPLTAMADIAEKQCPNIDYSNRFGPVRDQDGH
                     GYCYAFTAAALVEEHLCKTNQKFCGKSVAPLDISRCQWTLLKEREGGYVNNALDCAIN
                     QGGVCFEEFAPYNALTNFGCTLWDFFTGDGAKCKNKRIADLFTKWKKIALSTEGRKIK
                     RPFLKLSKN"
     gene            complement(17011..17286)
                     /locus_tag="VIG33_05550"
     CDS             complement(17011..17286)
                     /locus_tag="VIG33_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270333.1"
                     /translation="MKSMLIAIFMVQLIGNLKAFANTSNLIGQAVVCSRVLGVLGSSS
                     GIATEYKDGYLILKNTEYNGLLGTYYAIAKEADANSCKIISEQYGGF"
     gene            complement(17564..>17846)
                     /locus_tag="VIG33_05555"
     CDS             complement(17564..>17846)
                     /locus_tag="VIG33_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY8270334.1"
                     /translation="YYKFKSFLSPELVAADYHRAGLRMTSLGKPVGSKKDFEMMSLSV
                     SIVNACPVCVASHEKAVRSHDVSADKIHDLARLAAVSKGLTSLKKAMTL"
BASE COUNT         4915 a         3895 c         4089 g         4947 t
ORIGIN      
        1 ctatccgaat gttttcattg gacttctggt tgtactcaaa cagaagtaaa gtcgcgctac
       61 gagtttcaaa atgaataata agcgccttcg cgacttatga tagccgtatc ttcgtctagt
      121 agaaaattta agacccttct aaaaaacgga gaaccacaaa agaggtaaaa gaggcaatac
      181 gcgaccttat ccttttgaat cgatagattt cttcgaaaat ttaaacgaca cggtggtatg
      241 agaccctggt tttgaagagt aaacccaata ccacaaattc ccttttttta ctttgtagga
      301 accgttttcg ctaatccaat tatttatatc cttgaggagc tgattgagaa ctcctttggg
      361 atcgaaccaa atttttgagg tgtcaataaa gtctaaataa aaagaagaga gatgatctag
      421 ctgagattcc tgtttgagaa taaagctgac ttcaatatgg aatccgtcga ttttttttag
      481 atccgcattt aatttatttt caaggttagc ggtgagttga aactgatccc atcgagagtt
      541 tggtaaagtc tcaaagacca ataacaaatc cagatctgtt tcgtgtttta gtggagcttt
      601 tatggtagag ccaaattgta aaacgccgat caagcctgga aaacttttaa aatattgctc
      661 gacccaattg tggagtgatt gacttaaaaa gtcggcagct tgcttttgcg tcatcatatt
      721 tttgcaaatt ctggtttaaa cagataaatc atccattcta ctttattgta acaagtctgt
      781 gccattttga agtcatagtg ttccaagggg attaagtcat cttctccata aaaggcattt
      841 tctcgttctc ttcgtaaata ttttgaaaac tctgtaattt catcgagatt tgcgcggatc
      901 gactcagata ggagattttt attgtccgtt aaaatgcttc ctacatcatg ccatttcggt
      961 ggatcaatga gatttaagcg aagatacccc ttcagtagca actcgacaat ttcttgactt
     1021 tcacgaacga catcagggta ggctccctcg tccaatagaa ctttgagagc tttaaaccgc
     1081 atttggcacc gttttagata atcttgagct aagtcattcg ttgtcataac tttaaacctt
     1141 ggaattagtt taaactaatt aaatgcaatg tcattaactg tagcagcact gatatgaata
     1201 tacaactgcg gttatataat gaccgctccg gcctattttc ctcgcatcag atttctaaac
     1261 tctcccggaa gtggagaagt gaagaccata tcgcggccat tgagtctgat tcttaactca
     1321 gtcgcatgca aaaagaggcg ttctgttccg tacatcttga tgatgcgttc attatacttt
     1381 tggtcattgt agcgtttgtc gccaaccaca ggatgctctg ccacagccgc atgtttgcgg
     1441 atttggtgtt ggcgagcagc ggtaaggatc tcgcattcta cgagggttaa gtattcattc
     1501 ttttgcagca ccttcaccag ggtttcacat tctttccgat ccttgctgag accttgagga
     1561 ttttttcgtc cttccgcttt gtcagaaagt ttccaatccc aaagagcggt ctcttccttc
     1621 atcgggccgc gcagaatggc ttgataaagt ttatgggtgg aatctttttg catttcctcc
     1681 atgagctttg ccgccacttc aggatctttg gcgatcatga aaacgccgct ggtttctgaa
     1741 tccaagcgat gaaccgcatg aaatgtcaaa tttggatttt tcttttgttg ttgtaaaaca
     1801 ctcaacacgt tcaccttatc attgtgaacg gaaattcctg ccggcttgtc gataaccaac
     1861 cagtccgatg actcttgaat gatattgaga cttttcatta taacaacact ccgcgcagga
     1921 acatacttgc gaccgtaaaa taaataatta aacctgtaac atcgaccaga gtcgccaccg
     1981 caggagctga agctgaagcg gggtcaaaac ccattcttcg cagaagaaaa ggcagcatcg
     2041 accctgagag ggttccccac aacaccacac cgacgatact cacggccact gcgaaggcca
     2101 ctaaaacata gtgtggtcca tagagttgtt ctctggctgg ccagagtaag atccgggtaa
     2161 gtcccacaag acttaaacaa gttcccagcg cgaggccagt gagaagttca cggcctagaa
     2221 cccgccacca gtcacggagt cggacttctc ccagagccat tgcccggatg atcaaggttg
     2281 aagcttgaga accggagttt cctccagagc taataattaa aggaataaac agagccaaga
     2341 ccactgcgcg ttcgatttcg gactgaaagt atcccatggc tgtcgcagtg aacatttcac
     2401 ccagaaaaag aaccatgagc cagccagcac gtttttttat catctccaac agggagattt
     2461 tcaaataggg ggcgtccagg gcttcgaccg ctcccaactt gtgaatatcc tcagtggctt
     2521 cttcctgcag aacatgggcc acgtcatcga aggtcacgat accgaccata tggttattgc
     2581 catccacaac cggaatggcc attaagtctt ggcttgaaaa gactttagcg atctgctctt
     2641 gatcggtgtc gaccagaact ttaaccatgt cagttgtcat gatctcttta atctttttgt
     2701 ctggagaaga ggcgaataat tctctaaatg aaaccacccc taaaagggtt tgatcggccg
     2761 ccagcacata cgcatagtaa atcgtttcca catgggtttt tgcttggatg cggatatagc
     2821 tgatcgcttc atccacgctc atatcaggac gcaggcgaat aaaacgcgaa ctcatcaggc
     2881 cgccggcatt atcttcagca taagctaaaa gagcgatgac ttcgcgtttg gtttgaggat
     2941 ctaacagacc taatacctct tctcggtatt cgactcccat ctcctggatc aagtcggcaa
     3001 cgtcatctgg tgccaggagg cgaacccaag agcgcttttc aagaggactg gcttcctcca
     3061 ataattccac ttggtcatgg gttttgagac ttaaaaataa ttcctcggcc tcagtgcgcg
     3121 gaagttcttt aaatttttta cgccgttcgc cggtagggag ggcggaccag ttttcgaaga
     3181 gatcgtaaaa ttgctcttct gtttgagaga aggcttggga aaactcttta gagtcagcag
     3241 agccagaggg gtttttattt tcagagttca tgcaacctcc tcgaagtcta aactgggaga
     3301 ctacaagctt aacctcaaag gctaagccag taattttaag ctttgtactg cgatctccta
     3361 tcgacttcaa tcatttaatt ctaaagggat ttttttagag atcccttagc tcagatgagc
     3421 ggcttcaaag cggggaagga cttcactagt gggacctaga ctgtggattt ccccgttttg
     3481 cagccataat acacggtcac atttctccag agttgagagg cgatgggcaa tgatgatctg
     3541 agtgcggtcc gcgaagaact cctcggtggc tttgaccaat atttcctcgg actgaggatc
     3601 cacagaactg gtggcctcat ccataatgac gatcggggct tcctgtaaaa ggcagcgagc
     3661 catacagagc aactgtcttt cccccagaga taaatttttc ccccgttctt caattctcat
     3721 attgagaccc aaggcttgtt gggtgaccca ctctttgaga cccacttgat ccagaacggc
     3781 cagcagcctt tcttcggaga gctgattttc aatatccaaa ttactgcgaa ggctgccttg
     3841 gaataagacc ggatcctggg agatgaaggc aatggagcga cgatataaat ttaaatccat
     3901 tccggcttcg gagccctttg ctagaccctt gtctgagttt ttgattttag ggatgtttat
     3961 tttagggcta tggccgttga tggcaatgcg gcctccttgc agggggtaaa gataaaataa
     4021 tgcctgaatc aaacttgatt taccgcttcc agttcgaccg acaattccca ggcgctcacc
     4081 ggctttgact tcgaaattaa catccttaag aacccagggg agatcttcac ggtatttaaa
     4141 ccacaggctt tggacttgaa ccgaggcatt gcgttcttga gtcagacggc gattgtgcaa
     4201 atatttttct aaagtttcat catatttggg atgaccggta ttaaaatggg cggaagaggg
     4261 caacaagctc ccaggttcaa ggtcttttcg caaatattga tccaggcgtt cgacgccaat
     4321 catggcctct tcaaattggg tgagccactc aaagaacatc tgcactgtgt tggctgaaag
     4381 ggcgataaag ctaaaagcca cccccacgga acctacggaa gccagaccct gatgaaccat
     4441 ataataagcg gaaaccccgg tggtcaataa cagcaaggcc gatagactgt tcatctgaat
     4501 agaaaaacca atgactctgc cagtggtttt taatttttgc tgaagaaaat accgatccaa
     4561 acgagagaaa cgatccatga atgattcttg gcgacggaag gagcgaatgg tgctggctcc
     4621 ttgggtggtc tctgcgaaat gggcaatgga gggagagcgg ctggccgaga gatcacgacg
     4681 cgactgccgc aattttttcc gattcagata ataaactccg taatttaaca gagcgacaaa
     4741 cacgacaaaa gctagataat aaggactcgc gatggtgatc aatacaatca tcaccagcag
     4801 atcaaagata atggaacaga attccgccag gggaccgccg aaaagtctga acacatttcc
     4861 ataatcactg gaaaagcggg tgatgatcct tcctgcaggg gtggcatcaa aaaaactcat
     4921 aggcaaacga gaagttctta aagtcacttc atcataaatt tgactgatgg cttctgcgga
     4981 gagtcttgaa aaaatcaccc gaaagcagag tgttagaaca aagccaataa gagtcatgac
     5041 ccctaaaagt tgcaagtact tttccgacga gaattcagca aaaaaactcg gaagcggttt
     5101 gcattgaatt ccctgttgag gtttgcagaa actgtctacc cacacaccaa tgatattggc
     5161 gttggccaaa agtaaagtac gacctcccag gcccgcgaaa aaagtaaaaa tgattttatt
     5221 tttaaaggga cggtaagctt caccgagagt ttgtaaaaca gcacgactgt agccgctctc
     5281 ctgcttttgt tgttcgccat cagagaaata tttaggcttc gacatgctta gcctccacca
     5341 cgctctgcgt gtagtctcgg aagagcgggg atcgatccat taattcagca taggtccctg
     5401 aatccacaat agatccttct tgcataaaaa gaatacgatc catccggtgt aaaatactca
     5461 gacgatgggt gacgagcaaa cgggttctct ctgcccaggc gccatcaatc agttgtgtaa
     5521 atagtttctc ttcagtatcg acatcgacgg cacttaagca gtcatcaaga agtaaggtgg
     5581 gggctttatg gaaatgcact cgcgccaaac tgaccctttg tttttgccct ccggaaagat
     5641 tcactccacg ttcgccaatt tcagttgcca gaccattttc cactcgctct ttaaagactt
     5701 caaattgagc taagcgcaag gactcttcaa tatcgctgtc gcgttctatt tcaatgtcat
     5761 agaggaacgc gacattttcg cgcagactgg cactcatgat aaaaccttct tgggggacgt
     5821 aagcgaagtg cgcgcgcaga tcattcaacg tcatgtcggc cacactttta ccttgaagag
     5881 tatagacatc ggcagcggcc cctgtctctc cgagcagaga caacagcagc atggatttac
     5941 cagctcccac ttctccgacg atagcgacga gttctccttc gcggattttc agatggatat
     6001 ttttgaggat ttgctttcca ctgatttgta gattcagatt cttgacctca agggccaggt
     6061 tttcttttgc tttaaaaggt tcttttgagt ggtcttcagc agattcatga ccgtcgttct
     6121 cattcgtgat tcgcagaaaa ctttccaagc gttgtaaaga agtccaagag tcaaaagcaa
     6181 aagtgaaaaa ccacggcatt tgccggaagg ggcgggtgag gaagactccc acgatccaca
     6241 gcagcgccaa gagttcgcca ctggttactt ggcgcttcga ataataaatt aaagaaacca
     6301 aggtcaccac attgagcgca aaggtgatcg aggtggaaat ggagttcatg atttgtccat
     6361 tggtgaccat gatcacgcgg ttggcggttt cgacttcgcg tttttgaaaa atattttttt
     6421 caaaaagacc cagccagcct agaattcgaa tagtgcgaat attttgaatc cattcattca
     6481 ccagggcaat gcgttcaccg gccaaggttt tgaagcgata aaagaaacgg gattggcgaa
     6541 aggccatcca agtatttaag cccgtgataa ccatcattaa aagcaaagtg ggcagcatcg
     6601 gcaagtcata catcagggaa atcgcaaacg gagctaaaat taaaggaaat aaggtcgaag
     6661 ctcccgccgg aagagtttgt tctaagaaaa cggtggagcc tgggatatca gtggcataaa
     6721 gctgaatgat ttctcccacc gggcggctgc tcattgtatc cacccttaaa tccaaagtct
     6781 tctggtaaag acgatgggaa aataccgatt gcaaatgaat ggactcgcgc attcctaggt
     6841 agttggtgag ttgaaagaaa gcttgggaaa gtaaaatgca gaaaaaagcg aggagaataa
     6901 atattaaagg gctgccctga aaccccactg gcaagaggct ggcttgggcg gaaagatggc
     6961 ctgtcaggtt atcgataaaa agcttctgaa agtaagggcc aaacagggcc acaatcgtgg
     7021 ccactaaaga actcaacacg atgagaacgc gagcgtggaa tcgattaaaa aggacatcaa
     7081 aaatgagggg tttcatggtc tgcgatctta cctcttatcc gctcagagtc aaaggcctgt
     7141 gtgattcaac ggtcaaaata ttttttcctt tgcttccctc tcagatttgg attcagaata
     7201 agcaaatggc ccgcagctta gattttaaaa aaataatggg aattttgcag gaggagtcgg
     7261 aagagttttc ttcggaccgc cgtgctttct tgaaaaaaat ggtttatagc cccctttcag
     7321 ttcctatggc ctccttggct ttagcgggct gtagcagctt cgatagctgg gtgatagggg
     7381 ataataagca tctggatcaa gaagtgatgg tgttgggagg cggattggca ggcttgtctg
     7441 cggcctacca tctgaaaaaa aataagattc cttataaggt ttacgaagcc tccagccgag
     7501 tgggggggcg cattcaaacc ttatttcatg ccaatgaaga tgagcaattc gctgaattgg
     7561 gagcggagtt ctttgaagcc tcccacgagt tggtcattca gctttgtaaa gaattgaatt
     7621 taagcactca agatatttcc tatgatccca agctcaaccg cagcctttac tgtttgaatg
     7681 gtaaagttgt aaacgaaaaa gattttcgaa aaaatctgaa gcctctggct ctcaagcttg
     7741 ctcaactccg tcgtgagtcc tttgcatcct ttacttcaga aatcaacccc cgagctcttg
     7801 cgataaatcc ttcgatggtg gcattggatc aaatgtctct gttcgatttt gtgaaccccc
     7861 ttcacggaac catggatgag gctactttgc aaagctttga aaatctttgc atctctgagt
     7921 ggggagcgga cctgaaaaat atcaaccttt tgcattttct ggtgaagcta gacgtggaag
     7981 aacgaagttc cgcggcgtcg ccgcagaaaa tcttccgagt gcaagggggg atggctcgga
     8041 tagtgcaaac gctgggagaa cgcgttcaag gaattgtacc aaatttgact cttaagttag
     8101 aacatcaact tatagctatt cgcgaaaaat ccggaggcta cgaatgcact tttaaaacca
     8161 cgaagggatc agatacggtg tgggctcgcc aaatgatttg cactttgcct ttttcccttc
     8221 ttaaagaggt ggatggtatc caaagtttgg atttagggcg taggaaagat tttattgcca
     8281 atagtaacta cggtactcac gcaaaggtga tttgtagttt taaagattcc ttttggaaaa
     8341 agaaacaaaa cgcaaccctc gcttcccagg gtgttttccg gggacagcta gcgggtcaga
     8401 gttactggga cagttcgcgg ggtcaagccg gcacccatgg acttttgacc tcgcagcggg
     8461 gcggaagtgt gggtcaatcc acgggggcga gcgccgctca acagatccgt gaagatttgc
     8521 aatgttttta caaagagccg cttgcagaag aaaactttca tctcagcaat tggagccaaa
     8581 aaccctttgc taaggggtcc cgcttgaacc tgttacccgg tacctattta aaatatcttg
     8641 aaatactgct ggaagagaaa gaattggaag gtttttatat tggcggagaa cactggagct
     8701 tcgcccattc aggcactatg aacggtgcca tagaaaccgg agtggcagcc gctgagaagg
     8761 ctctgaagaa ggctttcttt aacggagtat ttaaataaac tttcatagtt caaattattt
     8821 ttgatttact ccgaagccac ggctccatta caatacgatg atagttcccc taagaagagg
     8881 ttgcggcgtg attttacgac ttcccctgtt ggttttcgtg ttggctctgg gtgggttttt
     8941 catccctgaa gcgagcaatg cttatcctga ctttatttcc tatggctata acacgtgcat
     9001 tacctgtcac tataatggtc aaggaaatgg gcctttaaat gattatggcc gggctctttt
     9061 tacaacagag attgcggcca gggatgtctt taacaaaggc ctctcggaag aagagattgc
     9121 ggcgaagtca ggttttctgg gctccacgga gcttccgtgg tggatccgtc ctggacttaa
     9181 gtaccggggc ctttatttta tcaacaatcc cggcagtgaa gccgcagtca aaaaatacgt
     9241 gatcatgcag acggatttaa atgtggccgt gcagtttgat ccaaaagcta aatatttatt
     9301 tatagcttcc ggagggtact cgcccacgcc cgcatcccaa caagcgaaca aggatcccaa
     9361 ggataaaaat tttatttccc gtgagcatta ttttcgctgg caagcgaata agaaattctt
     9421 ttcctatttt ggtctgatgg ataaagtcta tggtgttcgt acagtggatc atacggcttt
     9481 cagtcgtgtg gtgacgggga ttgcgcaaaa tgaccaagcc catggagtga ttggtcagta
     9541 ttatggcggc agttgggaac tcactggcaa tattttcttg ggaaatctcc ttcaggaaga
     9601 ggccaatgtt cgccaacagg gggcctctat catggctgag tatgacatgg cggaaaagaa
     9661 tcgagtggga acctcttttc ttttttctaa gaatgactat ttagataaaa cacgttttga
     9721 attgcactct aaactgggac tcgacaaggg aaacagtctt ctgacagaaa ttggttttgt
     9781 gaaggacaat cccaaattaa gccctagtaa taaatatggc ggctatttga tgatggaggg
     9841 ggtgtatctc atcacccgtg gctataatat tatttcacag attgaatatt acaatgcgac
     9901 tttgagtccg gagtcacccg atatgacaag atggaccttt gggctcttga tgtttcccat
     9961 gccccgcatg gagtttagaa caactttcgt aaatggccgt tctattacag atacgacagt
    10021 gacgaatgat ctatggatgt tgcaggcgca acttcatata tcactttagg aggggctgtg
    10081 aaaaaaatga tgagtgctat ttttgtgact ctaatggttt ccccggcatt ggctcaaact
    10141 tcacagaaaa attatttatc tacggaggcg gttgagagat atcttttaac gttggattac
    10201 aattcttggt ttgaaaaact cacgatcacc aactcctccg gcgaggagca gaaatcccaa
    10261 tctttgtact atggctttgg cgtgggttta gaaaaaaatt ggtatcagcc aaattggggc
    10321 tggggattag ggacaggagt tcttgctggt agcgccgtgg gcggagataa atcaggaaca
    10381 ctgacttatt ttcaagcgag agttccttgg tggggtgtca gactgacgcc ccgattattc
    10441 tatcgatgga atccgcgaac tgattttggc ttggatcttt cgtctttcta taaacaagga
    10501 aattggccag cgggaacaaa taatctcaca gcaaaatcag gcgctgattt aatcagtggg
    10561 gcttttattg atatgcggat acgctttaat caaaagatag aaatgatgca gagttttggc
    10621 gtgatttata aggatgaatc catttattgg cggataggat tggcttatcg cttgtagagg
    10681 agggacttat attcctaaaa tccaatcttc gatgacggta agctccgccc ccgagaggag
    10741 agttccgccc tcgggcatat ttccaggcgt gctagttcta ttgccttgga tttttgtata
    10801 gaggagcgaa ccactgaaat ttccggcgac cacaagccct tgactaataa aatcttgttc
    10861 attcatccca gcccacgctt gatgtgaagc gcgggtatgg caagtggcac aattcgtttt
    10921 gatgatctct tttgccgcta aaaatttcac actgccatcg gatgccccat aggtggtggc
    10981 atcataagta gaggaattat agattttccc gcaggccaat ggggccagaa gaccgaagat
    11041 tgtgaggccc gccctcaagg ctcttttcat gatccctcct caataaaaaa ctaagtatcc
    11101 gcagctaagg tcctttgatc ggaactgcaa tgtgaagaac cacttcgtcg tccacaccgg
    11161 ctcccatata tttcacatta gtgattttga aatcggcgag attcaatttg aaatcagcaa
    11221 gcagctcatt attgttgatt ttgtaagttc ccgtaatttc tttttcaata cctcggatgc
    11281 gaattttccc cgcgcctttg cccttctttc ctgtcgcgga aataagaatc gcttcaggat
    11341 atttatcggt ttccaaatgt ttgagtgtgt gtttatcccg aagctcaatc cctgttttga
    11401 aattttttaa attgaccacg atattccggg ctgaaacttc atcacctttt ttagtgacga
    11461 aacctttaat gtcagaggtc tttcctttaa aagaaccggc agggcttaaa ttaacatcga
    11521 cggtgacgga ggcggaccag gctatggtcg aaaacaaaag aacaggcata ggcagaaaga
    11581 gggcggccat tttcatgaat tctccttatg atcctatttt atctttgcgt gaggactgag
    11641 tgtagtcttc tgaggtccaa gtcctgcttt gaaatgctaa aaccctgtaa aaaatgggag
    11701 gaaatgcagc ggagaccagg tcctaggttt acagggccga gatcttttga gataaaggcg
    11761 gactatgaaa aagatcacta aaaaatcaaa gacttccctt taccatcact ttagcgcaca
    11821 ggatatctta tcccacgtgc tgcccactcc aatggttaaa aatggtttga gcgaagaaga
    11881 gaaaattgaa aaaatcactg aaaaattttc agaaatcatg gaggttttag gtctggattt
    11941 gagtgacgac agccttcgcg atactccacg tcgggtggcg aagatgtacg tgaaggagat
    12001 atttcgtgga cttaatccgg agcactttcc aaaaatgacc gtgatcgaaa acaaaatgaa
    12061 atacgatcag atgatctgcg tatcgggtgt gtccactttg tccttctgtg aacatcattt
    12121 tctgacaatt gatggcgtgg cgacggtggc ttatattccc aacaaaaaag tgattggcct
    12181 ttccaaaatc aatcgtattg tgcagttctt tagtcgtcgc cctcaggtgc aggagcgcct
    12241 gactaagcag attgcggatt gtctgcaata cgtgttggag acggagcatg tggcggttta
    12301 tatcgatgca aaacattatt gcgtcatcac ccggggaatt gaagacaccg cgagctccac
    12361 cgtgaccaca gaagtccgtg gtcacttccg cacgaaggtg caaacccgtg aagagtttct
    12421 ccttcagtgc aaacgaattc accgctaaaa tacaaaagtc cttataaaga attttgcaac
    12481 aattcgcggg aatttttcga aagtttatcg tgggcgacca tgcgatggat ttgctgcaaa
    12541 gcctgctttt tcatttgtgg tggaaacttg tttacaaaat taaaagccga gcacaaacgc
    12601 gccgccactt ggggattcct ctcatcaatt tttaagatca tgtccgtcaa aaattcataa
    12661 gcgggttgtt cggggttgtg gaaaataaca aagttttgtg taaaagaacg caaaagcgca
    12721 tagacattgt tgggattgtt aatattaaag ctaggatgtt gagtcaggct tttcaccgtc
    12781 tcaaaggtgt ctttgcgaga ggaaaaagct tgagcgatga accacttgtt catcaccact
    12841 gagtctccat gccatttttg atggaagtct tccagagctt ttggacgata gttgctgccg
    12901 ccgctggcca gaagctccag cgccaccaat tgatcagtca tgttttgagc cgtctgatac
    12961 tgctgcacca ccatttcggt cagggtggca tcgtccagtt cctgcaggaa gctcagcgcg
    13021 atatttttaa gaaaacgatg gccgaagact tttgggtcac ggctcaaagg ttccacattg
    13081 tggaatttac cgtaaatatc agcaaatgat ttcttatgag ccaaagctat ttcatggcgc
    13141 agctttttgc gtgcggcaaa aaaggcaggg gcgtcaagga tcggctgttc ttgagccagc
    13201 aaaatatccg acggcaattg caacatttga gctttaaagg cttcagacaa attttcatca
    13261 tgaaggatag tgccaatagc cgttatgaat ttattattta ccgtgagcgt ttgctttgct
    13321 tggtgctggg ctatcaagtc cgcgaataaa cgcaaaatta ttttttgagc cgcttcacgg
    13381 cgattgaatt catcgttgtc tttctccatt aagaaataca gatcctctag cggtcgctcc
    13441 cactgcaagt gtatcggggc tgaaaactcg cgcagcagag acagtacagg gcgctcggga
    13501 accaatttaa agacaaaagt ttccacgcct tcacgcagtt caattaaagt ttttccatcg
    13561 ctattgcgag tgatcttatt acaatgcagc tccatttctt tgccctgaga gttcaaaagc
    13621 cccatcagaa gaggaatatg gaatggtttc ttttccgctt gggtctctgg atgccccaca
    13681 gaagaaggat aggattgagc cagggtcaaa tgatattccc gcgcagccgc atcgtaattc
    13741 tcagaaacaa tcacttgcgg ggttcctgct tggctatacc agcgcttgaa ctgagtgaag
    13801 tcttggtgat tcggctcagc gatcgctgcg gcaaaatcct cggtggcgac ggcttgacca
    13861 tcatgacggc ggaagtattc atccatacct ttgcggaaac ccttttgtcc cacaatggtt
    13921 tgcatcatgc ggatcagttc cgctcctttt tcataaattg tggcggtgaa gaaattatcc
    13981 acagccatgc aagattcggg acgcacgggg tgggaattgg gccccgcatc ctcggtgaat
    14041 tggcgttctc gcaaggaatc cacatccaaa attctctgca cgcttcgaga agtcatatca
    14101 gcggaaaatt cttgatcacg aaaaacggtg agtccttcct taagacttaa ctggaaccaa
    14161 ttgcaaagag tcacccggtt gcccgtccaa ttgtggaagt actcgtgggc caccacggac
    14221 tcaatggcat gaaagtcggc atcggtggcg gagtgggagt cggcaagcac caaacgcgag
    14281 ttaaaaatat taagaccttt attttccatg gcccccgcat tgaaatcatc aatggcaacg
    14341 atcatgtaat cattgagatc atattcaagg ccgaaagtaa tctcatccca cttcatggat
    14401 ttttttaagg attccatggc atgccagcaa cgatcttgct ttccgtgagc ggcatagact
    14461 tcgagattta ctttgcgacc tgacttggta atgaagtggt cgcgaatgac gccgagatcc
    14521 cctgcaacca aggcaaaaag ataacagggt tttttgtggg gatcattcca gaaggctttg
    14581 tggcggccgt tgccaagatc ttcaacttta acgcgatctc catttgaaag cagcaccggg
    14641 tatttttttt tgtcagcttc aatggtgact gtatatcgtg tcattacatc ggggcgatcc
    14701 atgaaataag tgattttgcg gaagccttgg gcttcacact gcgtgcaaaa ggtctcattg
    14761 gatcggtaaa ggccttccaa ggaagtgttc ttttggggat agatttcggt ttccactttt
    14821 aactcgaagg cagtgggaac atttgcgatc tccagcccct cagaagttaa tttatactgg
    14881 gaagaagaga gtttttcatt attaatagaa acagagagca aatttaaatc cacgccttca
    14941 agaaaaagcg gagtgcctgc tttaagggat tttatctggc tcgtggcaag aactcgacaa
    15001 aagtcctcgt tgaggttgaa gttgagcgct aaggtgccca cttcaaataa gggtttggta
    15061 taatctttca agtaaatttt attgccttgt tttgtcatgt taaaacctcg gtccagatat
    15121 gttgtttagt gaggcattcc ctttagcggt ccttgactat ggttgataat aaagctggca
    15181 tattagaatg ggaattgttt ttacttctat cactgttaat acaccaaatc tagagaatat
    15241 gattcgcttc tatgagattc tagggtgtga atttagcaaa gtgaaagtca acattggagg
    15301 tgagcttttt cgcagctgtt tcgaaggctt tgaactctct ttgatgtcaa ttaaatcggc
    15361 tccgcgagaa ggtatgccca aggttatgat gggctttcaa gtgagtgatt ttgattcaaa
    15421 agtgaagctt ttggctttag ttccaggtgt ccttttaatt ctcgatccca ctgacatgcc
    15481 tgatggcaga aaagccatcg tgcaagatcc ggatggccat tccattgaac tcctgtccaa
    15541 agaaatgctc ctctaagggt ggacccgtgc ctgtggcagg acagagatat ggcaaattta
    15601 ccgatttgat ttccttgaaa ttttcattaa atcccagcca aaaataaaga tgtgatcatt
    15661 gaaatagcat aaagagactt cgtttctatt ttccatatag ccgattttag gagtgccctc
    15721 cattttttta cataaagttg aggtcggatt gactcccccg gggatccatt caatttcagc
    15781 cggtactttt attttcccga cgctgatctc ataagcaagg catgtttttt tgcgacactc
    15841 ctccgtgtcc gagattctgg tttcttgaat ggcaaaataa tttctttttt gatccctttc
    15901 cattttctca taaataaatt tttctgaagt ggcataggca aaattgcaga aaaaggatat
    15961 cgaaatgaag gtcttatttt tcactgagat acctcgcgct tagaatcgct ttttgaagaa
    16021 tatcaaccgg gatccactca ttaaaatctc cgccctcacc gtaactattt ctcagctgga
    16081 attcgcatgt atgtgaccaa gctcggtagc gcatacccgt aataacaagc ccatgaatgg
    16141 agttatttgg atacctgtac attcccgtaa gaccagcgtc catcactatc ccgacacttg
    16201 agttggactt taatccgtcc gcgataaagt ccgccatttg acgctgttcg tactcactat
    16261 tagattttgg cgcggttttc gattgtatag aggcgctgac ttgatttcga tttttttcac
    16321 aggcgctgga aacgagcgtg ttttttaaga aatcactttc tgacgatgag ttgttgaaga
    16381 gcgaccttag atcttttccc atcagcaact gctctggaac tttttctttt agggattgga
    16441 tcaatttttg ctcaatttca aaaatggcct tttgattttt ctcccctccg tcgagagagc
    16501 aatttttttc cacttggtga ataaatcagc aattcttttg tttttgcact ttgcgccgtc
    16561 gcccgtaaaa aaatcccata aagtgcaacc gaaattagta agagcattat agggggcaaa
    16621 ctcttcaaaa cagactcccc cttgatttat ggcgcagtct aaagcgttgt taacgtagcc
    16681 cccttcacgt tcttttaata aagtccattg gcaacggctt atgtccaagg gagcaacgct
    16741 ttttccacag aatttttgat tcgttttaca gagatgctcc tcgactaagg cggcagctgt
    16801 aaaggcatag cagtaaccat gtccatcttg gtcgcgcact ggaccgaacc gatttgaata
    16861 atcgatattt ggacactgct tctccgcaat atcagccatt gctgttaaag gaaaggtgag
    16921 agccaccaag aaacgaatta aggttaccat tctttggatt ttaggcagtt ttcacgcccc
    16981 tcacgagtcc agaaacgcga aatgataggt ctaaaaccca ccatactgct cagatattat
    17041 tttacatgaa ttagcgtcag cttcttttgc aattgcatag taagtaccaa gtagcccatt
    17101 atattcagta ttttttaata tcaaataccc atctttatac tcagtcgcga tcccactgga
    17161 cgaacctaaa acacccaaaa ctctactaca gactacggcc tgtccaatta aattagatgt
    17221 atttgcaaat gccttgaggt ttccaatcaa ttggaccata aaaattgcaa ttaacattga
    17281 tttcataact tttaataccg ctccaaatcc tatggaattg atttcccaat ggggatacgg
    17341 ctcctgattg actcataatg cgatatctat gccaatgctt atgattggta aatcgtcagg
    17401 agttcccggt cagagccaag taaagatgaa taatgctggg catttagagg tttaaagttt
    17461 gcgacctctc ctcggagtca atacggattc tcttttgatg aacttctgta cacaggtaac
    17521 gatttatagc cacacacgca gccgcctctg ccgcctctgc gttttagaga gtcattgcct
    17581 tcttcaaact agtcagccct ttagagaccg ccgcaagtct ggcaagatca tggattttat
    17641 ccgcagaaac atcgtgactg cgaacggctt tttcgtggct ggctacgcag accgggcagg
    17701 cattcacaat agagaccgac agggacatca tttcgaagtc ttttttgctg cccacaggct
    17761 ttcccaggga cgtcatgcgc agacctgcac ggtgataatc tgccgcaacc agttctggtg
    17821 aaaggaaaga tttgaatttg taatag
//
LOCUS       DATBTE010000147        15444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1658215, whole genome shotgun sequence.
ACCESSION   DATBTE010000147 DATBTE010000000
VERSION     DATBTE010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 15444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15444
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1658215"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..867)
                     /locus_tag="VIG33_05560"
     CDS             complement(73..867)
                     /locus_tag="VIG33_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY8270335.1"
                     /translation="MTTSITEITSSSNELFKAWKNLDSAKGIRKAGEFFLMGEKLIQE
                     FTQNKALQEKFPVCAELFTEGLQPLSLPNLRTANFKSFKLAKGLFEEVDFIGTHFNLL
                     LLELPKIPPFDPSTPPQGLELICPLGDPGNLGAVTRSALAFNASKVILSEEAAFPFAP
                     KVVKASAGAVLQMPFYRGGNLASFAESAVYALDMVGSDIDVFKWPKDLRLLVGEEGSG
                     LKNLKGIMRLKIDTGSVESLNATVAASIALYSYRHSQTRQKARNYK"
     gene            complement(864..1730)
                     /locus_tag="VIG33_05565"
     CDS             complement(864..1730)
                     /locus_tag="VIG33_05565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HEY8270336.1"
                     /translation="MAHKNKTNYSPYKARQEARHKGSTSISMEGPKALLGKHKKPQTI
                     YSLAEANDRLADIFRNHDFSCTHEERMKLAHFYRLLMENQEKENFTRLLKLRDIAIKH
                     YIDCMIVSELTDLKFPLLDVGTGPGFPGIPLKIRYPKEKIILAEGVQRRVEFLKHVRQ
                     EMKLEELDIVGRNINKHFVYPVNGVITRAVEDIRNTLGNVLSCLQLGGRVYFMKGPGV
                     DPEIPMALKEYGQYYKLIEDIPYSLPETPHNRRLVIFEKIQHAPLPENDDDEDLLLEE
                     LSNEERRRWSTR"
     gene            1839..2864
                     /gene="pheS"
                     /locus_tag="VIG33_05570"
     CDS             1839..2864
                     /gene="pheS"
                     /locus_tag="VIG33_05570"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEY8270337.1"
                     /translation="MLQTKLDTIKEQALAQFKAASNVKALYDLKVQFLGKNGSLTEIM
                     KEMAGLSKEEKPLFGKKVNEVKGALEQVYVEVEENLKKAEVESKMASEELDLTLPSYT
                     RPVGTQHPVYKVIEDIYGIMSRLGYSVRTGPLIEKDYYNFEALNIPADHPARDMQDTF
                     FIDPTHVLRTHTSPIQIHSLEREKPPLRIIGTGPVFRFDSDISHLPNFHQIEGMCVDR
                     KVSMADLKGTISFFVREFFGPGLKTRFRPSFFPFTEPSAEVDCSCPICKGKGCSLCKH
                     TGWIEIGGCGLINPKVFEKAKISYPEWQGFAFGFGIERMGIIKYGIDDIRLFPENDVR
                     FIRQFLP"
     gene            2861..5308
                     /gene="pheT"
                     /locus_tag="VIG33_05575"
     CDS             2861..5308
                     /gene="pheT"
                     /locus_tag="VIG33_05575"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY8270338.1"
                     /translation="MKISLKWLQDFVDINDYMKKPEELGDLLTRAGLEVEEITNRARD
                     YEHVVIGLILEKDKHPNADKLSLCKVTTGDGVVHQIVCGATNHQANDRVVVALPGAVL
                     PGNFTIKKAKVRDVESGGMLCSYKELGIAKESDGIVILPTDAPVGKGFAEYSGYDDVV
                     LELKVTPNRADCLSHYGLAREIACVVGRELKPLSSKLVLSNDSTKKQISLEVSNPEYC
                     PRYAGRFIKGVQVKPSPEWLVKRLESVGLNAINNVVDVTNYVMMELGQPLHAFDAKNI
                     EGGKIVVSNAQVGEKFKTLDGTEVSLKGTELMIRDASRPVAMAGVVGGQNSGVSDSTT
                     DIFIEAAYFLPMVVRKASRSHGVETDSAYRFSRGVDPDGTVRAMDRAADLIVQIAGGT
                     AFGDHHDFYPHPVKKSLVEISVQTVSDRLGYPADENKFVDYMRRLGCQVNNGLADNKK
                     YGILPPTFRFDLEQDMDLVEEYARLNGYENIPESLPVFAESPAFQDKVFTLNMKTSQI
                     MRGQGFSQAFNYAFIGGQTQKKFLGGIEGLKAAGFLASEAEIQVLNPLNEETDTMRSS
                     LSIGLFKNLSNNFNQGNHFGRLYEMGQCFYKENEEYKETAHLGLAAWGYSTHLWNQTP
                     THPLVFEVKAAVEALLKNLNIGAYSWVTPADRGEIPHFLHRGQFAHLVVEGKKVGYLG
                     SLHPVILDDHKIRVPGAMAELDLDQLYKGQPRPSRFETLSKFPAVERDFALVMAKSVR
                     VGDVVREIRKQGGGILNSVDIFDVYEGEKMEAGKKSVAIRLTFQDKNATLQDVQVVEA
                     SNKILETLKKQFSLTMR"
     gene            5402..5755
                     /locus_tag="VIG33_05580"
     CDS             5402..5755
                     /locus_tag="VIG33_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEY8270339.1"
                     /translation="MAGQNLGKSTVTKADIVEKVYQKIGFSKKEASELVESVFDTLKD
                     VLQNGEKVKISGFGNFVVRGKNERIGRNPQTGDQITISARHVLTFRPSQVLKAMLNGE
                     EYAHLKDDDDDDDEN"
     gene            5712..6278
                     /locus_tag="VIG33_05585"
     CDS             5712..6278
                     /locus_tag="VIG33_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY8270340.1"
                     /translation="MPTSRMTMMTTTKTSLEIELTGTEQLELHLGDLHVEELALAPDI
                     LNNPLVIAAPLSLPAALCDDKLLAEINSIPDKMGFKIGDVADILGIKQYVLRYWETEF
                     DLLKPKKASNNQRMYTRKDVENAFLIRKLLHRDRFSIEGARNALKELKAHVKKEKDYS
                     QVVHRLDSVHDRLEDLVIDIRKVRQLFK"
     gene            6397..6795
                     /locus_tag="VIG33_05590"
     CDS             6397..6795
                     /locus_tag="VIG33_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270341.1"
                     /translation="MKTTLAILTFLFSANVFAKTITLTCVMGDIGDMSIKIEDQGEGK
                     EKLSVILTAMDGVTVRTYVNNNFLNQAASQGLKEGKIQAILSKTNLVASFGGAFLDAG
                     VLELNRNRETQKNEVIFAAEGLVYVATCAQ"
     gene            6921..7706
                     /locus_tag="VIG33_05595"
     CDS             6921..7706
                     /locus_tag="VIG33_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY8270342.1"
                     /translation="MKRKPWPIILLALLHVFAPLGNLLMNSYRSGRSIELTWNFWHYT
                     LPKSLFFTYVFLPPLAGVLIYICRRWSYWCYVGCLSLIFLGNVYGFWTSVNWINFVTL
                     FMVLLVDVLVVAYFVVPSVRQVYFDPRMRWWETAPRYDFEVPAAMNGQASVIKNISEG
                     GALVEAPSSYSEGQGVELTWSYKGESFSLSGKVVYRKPVNSLYGFGVRFDSTSQVQKT
                     MKPLIALLHKEKRMIRNLPGPEDSFTAWVKKLIVNREGLFPKR"
     gene            complement(7731..8711)
                     /locus_tag="VIG33_05600"
     CDS             complement(7731..8711)
                     /locus_tag="VIG33_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270343.1"
                     /translation="MKKFFAMTALAIILGGNISFANTKVISDSLVNNLSCNSLSIGTI
                     LIRIRQEAFSSVNHLPFKNWGFHSGSFKLAACWGMGSTQRKLLYLLRLNEREAPAPNA
                     KSVLDMVRGATLKTLFDKHGSSYLMDENLKAYHVVPISDKNLREEYEHNTGTSFLEVL
                     FKGVDYQLDSFPIHRNLRTEIERSEELHFFRVKNIGMGAGSGPLSPHKNNVTVQHVMN
                     NLRLHRLTLVNLRLTRTIQHVVMAKSFTEDGKGNVWIQAYDSNQPEADQPIYYSKATG
                     HFYAPRIMGSFVGDTTGTDYTHPLGTFIVDEEERAQIEESLLKHYQQLCK"
     gene            complement(8850..10139)
                     /locus_tag="VIG33_05605"
     CDS             complement(8850..10139)
                     /locus_tag="VIG33_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEY8270344.1"
                     /translation="MSETFLAIDFGTSNSLVGAFHKNQRIEALKLDPKATDPTLMRTL
                     LYFPHADLCYYGAEAIEQYIEQDMEGRLFRSFKSHLPNQAYLGTILSNRILTLENLVG
                     IFLLELKKRAEALLGCPIENAVIGRPARYSMDPVADGFALHRMQKATQFAGFKNIQFV
                     PEPLAAAFDYRRQLTTEKIVLIGDFGGGTSDFTLIRLSPEKFRREDVLSIDGCPLAGD
                     ALDSIFMSHRLNEYFGAKSRYRFPLSSNVLTMPAGVTQRLNHPAHIVHLKEKETYEFI
                     REIGKCAITEKDKAAIERLFVLIEDQQIFPFFETIEKTKRELSDHPLSSFKFAYPGIE
                     IYESFSQSQFVDWAESTKEAIFAGLDRCLSSAGLTSQQVDLVCLTGGTAKVGFIQKEL
                     EKRFGRERLQAQSHFHSVLSGLIESARFWSEGLKVTE"
     gene            complement(10192..10962)
                     /locus_tag="VIG33_05610"
     CDS             complement(10192..10962)
                     /locus_tag="VIG33_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270345.1"
                     /translation="MAKKSFLNFQVDHMTLLLQPDLYKVSYLAFHTVFGVGPEDLLYD
                     KRKEWVPGEGEKSMTYAICLGRGANPDPELNNTIIAVVQPTEPKTQSSHVREMLDGHQ
                     QSAHWQHIALRTPDLLAFHQHAVERGVQFITPILKDEEEDLIQVFSGEWYLPGLKPSG
                     MFFEFLQRNPSEEHVKTAQSMNRESWFRDKTFLGLYGEKEREYASGAVTPFLKFDLFN
                     LLLKEFKEKQIWQITPADLQRAEDMMVEHTKNTTNVQG"
     gene            complement(11033..12598)
                     /locus_tag="VIG33_05615"
     CDS             complement(11033..12598)
                     /locus_tag="VIG33_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270346.1"
                     /translation="MKRTAFFPLLISALMSVVSSGAYANYCAAIRGNGELMPAHWGAM
                     SSIVEEQGLPAAMAGGSSASITMFLLESISLNPLPQNNSEKALLIKSFQGYLEALSQT
                     PEGKAIQSLLSDKSAFQSIIATSPKLDEALANPANKALLVKHLGSLQALMASEDLKEI
                     LNPEFILYVKRTLAMAKLQEDSLKPIVNYRVNQIKQAFKNFGKFDAKTDKTLFLRPGL
                     INFASLGRILGKMADFYAGYNLQSATGKQVQQLVRNFLDLCTPESKNMTWAELNQQRP
                     ACRQLLGGAILMYREAKNSSQSRVNDLVGSHIPSFPTTSVLYGKAVEQFTQLYVDYQT
                     NPDPNFGDFTVNPEELRFGYWGKPADLNAIENKIRTAAEYKDDAKSQKFLSLGQAPWL
                     NVLSTSPAEPGLSHIVALSRTELSAGGWSDLHPVMVLKAYGCEKIIYLTRKGEESPFA
                     QGVFQRLIKADEKTVEQFYGLENLNSSMMRSQANATQIKCTDWNHFDVRADMNGLVAE
                     AMRAPLLDPPYCK"
     gene            12865..13311
                     /locus_tag="VIG33_05620"
     CDS             12865..13311
                     /locus_tag="VIG33_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEY8270347.1"
                     /translation="MIYVDADGCPVKDEIYKVAERFQIEVILVANKTMNIPFNKLFRM
                     EVVSSGFDAADDWIVEQATHRDLVVTGDILLAERCVKKACRVLGHKGIEFNEDNIGMS
                     VANRELMQNLRHMGEVRGGPSPMDKNARSCFLSNLDQILHALKKAR"
     gene            complement(13313..14524)
                     /locus_tag="VIG33_05625"
     CDS             complement(13313..14524)
                     /locus_tag="VIG33_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY8270348.1"
                     /translation="MTISTFKDTRQRRKGLVLTGGGARAAYQVGVLRGISDIIRVKTN
                     PFQIISGFSAGAINGTWIASRHEEFEQATRNLWEGWASLTTEQIFKTAPLPLMNIGLR
                     WIKDRSKGGMGKKQQITYLLDTAPLNHFVRSKINFDALNSYLRAEKIHGVSVTAANYH
                     TGHSTTFFSGSAQIQDWQSLNRISRRSDLAAHHVMASAAIPVFFPPVRINDAFYGDGM
                     IRLNAPLSAAIRMGAESLLVIGIRGPSSTTPANPQTKETISLSEIASAILNGLFFDSL
                     DADLARIARINRTLSVMTPSELQREPDHLRNIPVLSLRPSEEVGNMPACALSSIPFTL
                     RFLLEGIGLTEEKSKDLLSYLSFEPRYMRSLLELGYEDTLQRKDQILEFFSEPEHPEQ
                     KLPEQERPLNT"
     gene            complement(14688..>15444)
                     /locus_tag="VIG33_05630"
     CDS             complement(14688..>15444)
                     /locus_tag="VIG33_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270349.1"
                     /translation="MVGDGDGSRICVEGLNSPQVKLPEGFHITWIGNSLQTAEEYIAS
                     RDGDRYIHKIVPFYQRGMIKGIPGHVQSWELRPDSKMHLTTTDKTTGKSFETVGDMVI
                     DATGYQDTVSQILSSAGAQAKTADITGPLPELNLTTTLLGRQALAENGEALPIFMIGA
                     AASALATREELAGSPNKNPIAIFNTVARTSMLMSQLTNTKPASSSRGLRPGRPPVQPA
                     AKLIENAQKHRRQQINRPLSLNVERKLLLRMVS"
BASE COUNT         3994 a         3141 c         3959 g         4350 t
ORIGIN      
        1 taaagacaaa aatgctgtct aacttctttt cagcgccgcc gaagcttcaa aaaacggagg
       61 gcctctcacg gattatttat aatttcttgc cttctgccga gtttgggaat ggcgataact
      121 gtaaagagca atgctcgcag ccacagtggc attcagagat tccacagatc ccgtatctat
      181 tttcagtcgc atgatgcctt tgagattttt aaggccagag ccttcttcgc caaccaacag
      241 tcttaaatcc ttgggccact tgaagacgtc aatatcgctt ccgaccatat cgagagcata
      301 gactgcgctt tccgcgaagc tggccagatt tccgccgcga taaaaaggca tttgcaagac
      361 cgcgcctgcg gaagctttca ctacttttgg tgcaaaagga aaggcggcct cttcgctgag
      421 gatgactttg ctggcattga aggccagggc cgagcgagtg acagccccta agttgccggg
      481 gtcgcccaga ggacaaatca gctctaagcc ttggggcggg gttgatggat cgaagggtgg
      541 aatctttggt agttccaata aaagtaaatt aaagtgagtt ccgataaagt cgacttcttc
      601 aaaaagccct ttagctagct taaagctttt aaagtttgcc gttcgtaggt tcggcaaact
      661 taagggctgc agaccttcgg tgaataactc cgcgcacacc ggaaactttt cttgcagagc
      721 tttgttctgc gtgaattcct gaatgagttt ttcgcccatg aggaaaaact ctcccgcttt
      781 gcgaatgcct ttggcgctat ccagattttt ccaagccttg aaaagctcat tggaagaaga
      841 agtgatttca gtgatggacg tagtcatcgg gtgctccagc ggcggcgttc ttcgttggat
      901 aattcttcaa gtaaaagatc ttcatcgtca tcgttttcag gcagcggggc gtgctggatc
      961 ttttcgaaaa tcaccaagcg gcggttgtgg ggagtttcgg gcaagctgta gggaatgtct
     1021 tcgatcagtt tgtagtattg gccatactct ttcaaagcca tgggaatttc aggatccact
     1081 ccggggcctt tcataaagta aacgcggcca cccagttgta agcaggagag tacattgccc
     1141 aaggtgttgc ggatgtcttc cacggcacga gtgataacgc cattcacggg ataaacgaaa
     1201 tgcttattga tattgcggcc gacaatatcc agctcttcga gtttcatttc ttggcggacg
     1261 tgtttgagaa actccacgcg acgttgcacg ccttcggcga gaatgatttt ttcttttgga
     1321 taacggattt tcaagggaat gccggggaag ccaggtcctg ttcctacgtc taacaaggga
     1381 aattttaaat ccgtgagttc agagacgatc atgcaatcga tgtaatgttt gatcgcaatg
     1441 tcgcgcagtt ttagtaagcg cgtgaagttt tccttttcct gattttccat taacaggcga
     1501 tagaagtgag cgagcttcat gcgctcttca tgggtgcagg agaagtcgtg attgcggaaa
     1561 atatcagcaa ggcgatcgtt cgcttccgcc agtgagtaaa tcgtctgagg cttcttgtgc
     1621 tttccgagca aggccttggg gccttccata gagatggaag tgctcccttt atgacgagct
     1681 tcttgacgag ccttgtaggg actgtaattg gttttatttt tgtgcgccat gcctatgtag
     1741 ttcgcctttt taaagtctct cgtcaactaa aattgcgcat cacattatca tgctaaatcg
     1801 ttgaaaatac tatagggttc agacataaaa tgaagtctat gttacagact aaactggata
     1861 caattaaaga acaggcgctg gctcaattta aagccgcgtc caatgtaaaa gccctttacg
     1921 atctcaaggt gcagtttctc ggtaagaacg gaagcctcac tgaaatcatg aaggaaatgg
     1981 cgggtctttc aaaagaagaa aaacctcttt ttggtaaaaa ggtaaacgag gtcaagggag
     2041 cccttgaaca agtttatgtt gaggtcgaag agaatctcaa gaaggccgaa gtggaaagta
     2101 aaatggccag tgaagaactg gatttgactt tgcctagcta cacgcgcccg gtgggaactc
     2161 aacacccggt ttacaaggtg atcgaagata tttatgggat tatgtcacga cttggttaca
     2221 gtgtgcggac cggtccgctg atcgaaaaag attattataa cttcgaggct ctgaatattc
     2281 ctgcggatca tccggcgcgg gatatgcagg atactttttt tattgatccg acccatgttt
     2341 taaggaccca caccagtcca attcaaattc attccctcga gagggagaag cccccgctgc
     2401 gcatcatcgg gaccgggccg gtcttccgtt ttgacagtga tatttcgcat ttgccgaatt
     2461 tccatcaaat tgaaggcatg tgtgtggatc gcaaagtttc catggcggac ttgaagggca
     2521 cgatcagctt ttttgtgcgt gagttttttg gtccaggtct gaagactcgc ttccgtccaa
     2581 gtttttttcc cttcactgag ccttctgcgg aagtggattg ctcttgcccg atttgtaagg
     2641 gcaaaggctg cagtctttgc aaacacacgg gctggattga gattggcggt tgcggtctca
     2701 tcaatcctaa agtgtttgaa aaggctaaaa tttcttatcc tgaatggcag ggctttgctt
     2761 ttggcttcgg gatcgagcgt atgggaatta ttaaatacgg catcgatgat attcgtcttt
     2821 ttccagaaaa tgatgttcgc ttcataaggc agtttttacc atgaagatca gtttaaagtg
     2881 gcttcaagat tttgttgata tcaatgacta tatgaaaaaa cctgaggagt tgggcgattt
     2941 gctcactcgt gcgggccttg aggtggagga aatcaccaat cgggccaggg actatgagca
     3001 tgtggtgatc ggtttgattc ttgaaaaaga caagcatccc aatgcggata aattgtcttt
     3061 atgtaaagtg accaccggcg acggcgtggt tcatcaaatc gtctgcggcg caaccaacca
     3121 ccaggccaac gatcgcgtgg tggtggcttt gcccggagcg gtgttgccgg ggaatttcac
     3181 cattaagaaa gccaaagttc gcgacgtgga gtcgggcggt atgctctgtt cctataaaga
     3241 actgggcatt gctaaagaga gcgatggcat tgtgatcttg ccaacggatg ccccagtggg
     3301 aaaaggtttt gcggagtaca gtggttacga tgacgtggtg ctggagttga aagtaactcc
     3361 gaatcgtgcg gactgtttga gtcactatgg tttggctcgc gaaattgcct gtgtggtggg
     3421 tcgagagttg aaacctcttt caagtaaatt ggtgctttcc aatgactcaa caaaaaaaca
     3481 aatcagtctt gaggtgagca atcctgaata ttgtcctcgt tatgcaggac gttttataaa
     3541 aggagttcag gtcaagccaa gtccggaatg gctggtgaag cgcctggaat ccgtagggct
     3601 taatgccatt aataatgtgg tggatgtcac gaactatgtg atgatggagc ttggccaacc
     3661 tcttcatgct tttgatgcaa aaaatatcga aggcggcaag attgtggtga gcaacgctca
     3721 agtgggcgag aagtttaaaa ccctggatgg cactgaagtg agcttgaagg ggacggaact
     3781 gatgattcgt gatgcgagtc gtcccgtggc gatggccggg gttgtgggcg gacagaactc
     3841 gggggtcagt gattcgacca cggatatttt tattgaggcg gcttacttct tacccatggt
     3901 ggtgagaaag gcttctcgca gtcatggagt tgaaaccgac tcggcttata gatttagccg
     3961 aggcgtggat cctgatggga ccgtcagagc catggatcgg gcggcggatt taattgtgca
     4021 aattgccggt ggcaccgctt ttggtgacca tcatgatttc tatcctcatc ctgttaagaa
     4081 aagtttggtc gagatcagtg tgcaaacggt gagtgatcgt ttgggctatc cagctgatga
     4141 aaataagttt gtggattata tgcgtcggct gggctgccaa gtgaataacg gtcttgctga
     4201 caataaaaaa tacggtattt tgcctcccac cttccgtttt gatcttgagc aagatatgga
     4261 tctggtcgaa gaatatgccc gcctcaatgg ctatgagaat attccggaat cgcttccggt
     4321 gtttgcggaa agtccggctt ttcaagataa agtatttacc ttgaatatga agacgtcgca
     4381 aattatgcgg ggccagggtt tttcccaggc ctttaattat gcttttatcg gggggcagac
     4441 gcagaagaaa ttcttaggcg ggattgaggg ccttaaggcc gcgggcttct tggcttctga
     4501 ggcggagatc caggttttga atccgctgaa tgaagagacg gacaccatga gaagttctct
     4561 ttccatcggg ctttttaaaa atctttcgaa taattttaac caaggaaatc atttcggccg
     4621 tctttatgag atgggccaat gtttctataa agaaaatgaa gaatacaaag agaccgcaca
     4681 tttggggctt gcggcttggg gatacagcac tcatttgtgg aatcaaactc cgactcatcc
     4741 gttggtcttt gaagtgaagg cggcggtaga agctttgttg aaaaacctta atatcggtgc
     4801 ttattcctgg gtgacacctg cggatagagg ggaaattcca cattttcttc atcgcggaca
     4861 atttgctcat ttggtggtgg aaggtaaaaa ggtcggctat ctaggaagtc ttcatccggt
     4921 cattctggat gaccataaaa tccgtgtgcc gggggccatg gcggagctgg atttagatca
     4981 gctttacaaa ggtcagcctc gtccttctcg ctttgaaact ctctcgaaat ttccggcagt
     5041 ggagcgtgat ttcgctctgg tgatggcgaa atcagtgcgg gttggggatg tggtcagaga
     5101 gatccgcaaa cagggcggag ggattcttaa tagcgtggat atttttgacg tctatgaagg
     5161 tgagaaaatg gaggccggga aaaaatccgt ggccatccgt ctgactttcc aagataaaaa
     5221 tgccacgctg caagatgttc aagttgtgga ggctagtaat aagattttag agacattgaa
     5281 gaagcaattt tcgttaacaa tgagataaat tcttgattgt tttcaaagac ttgttagcct
     5341 tgaaaagtag ttttgcgttt gagatcatat tttgggattt agatctaagg gagtttttgc
     5401 tatggcaggc caaaatttag gtaaatcgac tgtgacgaag gctgatattg ttgaaaaggt
     5461 ctatcaaaag attggttttt caaagaagga agcttccgaa ttggtagaat ccgttttcga
     5521 tactctcaaa gatgttttgc aaaacggtga gaaagtaaaa atctctggtt tcggtaattt
     5581 tgtcgtgcgt ggcaagaatg aacgcatcgg ccgtaaccca caaaccggag atcaaattac
     5641 aatcagtgct cggcacgtac ttactttccg ccccagccaa gtgctgaagg caatgctcaa
     5701 tggtgaagag tatgcccacc tcaaggatga cgatgatgac gacgacgaaa actagtcttg
     5761 aaattgagtt aacggggaca gagcagttag aactgcattt gggtgatctt catgtggaag
     5821 agttggcatt ggctccggat attcttaaca accccttggt cattgccgcg cctctttcgc
     5881 ttcctgcagc actttgtgat gacaagttgt tggcggagat caattccatt ccagataaaa
     5941 tgggattcaa aatcggggat gtcgctgaca tattaggaat taagcaatat gttttgcgat
     6001 actgggagac agagttcgat cttttgaagc ccaaaaaagc ttcaaataat caacgcatgt
     6061 acactcgcaa agatgtggaa aacgcttttt taatcagaaa gcttcttcac cgtgatcgct
     6121 tttcgattga aggggcgcgc aatgctttga aagaattaaa agctcacgta aaaaaagaga
     6181 aggattactc tcaagtggtt catcgcttgg acagtgtgca tgatcgtctg gaagacctgg
     6241 ttatagacat tcgtaaggtt cgccaattat ttaaataagt aagctgggaa tttaaaatgt
     6301 aaattttccc tgtctaaaaa actgacatct attgtttcta aaaaattcgg cgtattcttc
     6361 tcgagtattc aaacttgagg ctatgccgga gaaattatga aaacaacttt agcgattcta
     6421 acttttttat tttctgcaaa tgtatttgcg aagaccatca ctttaacttg tgtgatgggc
     6481 gatattggtg acatgagcat taaaattgaa gatcagggag agggtaaaga aaaacttagt
     6541 gtgattctga cagccatgga tggggtgacg gtacgcactt atgtgaataa caacttctta
     6601 aaccaagcag cttctcaagg gcttaaagaa ggcaagatcc aagcgatcct ttccaagacc
     6661 aatcttgtgg cgtctttcgg aggagccttt ctggatgcag gcgttcttga gttgaacaga
     6721 aatagggaaa ctcagaaaaa cgaagtgatt tttgcggcag aaggtctggt ttacgttgct
     6781 acttgtgccc aatagaaagc aatgtctctc ataaaactag acctgacttc gccagcagtt
     6841 ttcaaagaag tgcagttggg ttgaagagcc gtctgtaaca gtcgctttac aagatgcgct
     6901 aaagctaata ttcttatttt atgaaaagaa agccttggcc catcatcctt cttgccctcc
     6961 ttcatgtgtt cgctcctttg ggaaatcttt tgatgaattc ttatcgttca ggtcgctcga
     7021 ttgagctgac ttggaatttc tggcattata ctttgccaaa gtccttattc tttacctatg
     7081 tttttttacc tccgcttgct ggagttctta tctatatttg ccgtcgctgg agttactggt
     7141 gctatgtagg gtgtttaagc cttatttttt tgggaaatgt ttatggattt tggacgagcg
     7201 tgaattggat taattttgta actttgttta tggtgctgtt ggttgatgtt ctggttgtgg
     7261 cttattttgt agtgccttca gttcgtcagg tttatttcga tccccggatg cgttggtggg
     7321 aaacggctcc tcgttatgat tttgaggtcc cagcagccat gaatggacaa gccagtgtta
     7381 ttaaaaacat ttccgaaggt ggcgctttgg tagaggcacc gagttcttat tctgaaggtc
     7441 aaggtgttga actgacctgg agttataaag gggagagctt ctctctgtcg gggaaggtgg
     7501 tttatcgaaa gcctgtgaac tccctttatg gattcggagt tcgttttgat tccacctctc
     7561 aggtgcaaaa gaccatgaaa cctttgattg cgctcttgca taaagaaaag agaatgattc
     7621 gtaacttgcc cggacccgag gatagcttca ccgcttgggt gaaaaaatta atcgtgaacc
     7681 gcgaaggact gtttcctaag agatagaaac ttggattttt ttggtctctt ctatttgcaa
     7741 agttgttgat agtgttttaa gagggactct tcgatttggg cgcgttcttc ttcgtctacg
     7801 atgaatgttc ctaggggatg ggtatagtcc gttccagtgg tgtctccgac gaaggagccc
     7861 atgattcttg gcgcgtaaaa gtggcctgtc gcttttgaat aataaatagg ttggtcggct
     7921 tctggttgat tggaatcata ggcttgaatc caaacattgc ccttgccatc ttcagtgaaa
     7981 gacttggcca tcactacgtg ttgaatggtg cgggtcagac gcagattgac gagagttaat
     8041 ctgtgcaggc gaagattgtt catcacgtgc tggacagtga cattattttt atgcgggctt
     8101 agcggtccag agccggctcc cataccgatg tttttaacgc ggaagaagtg gagctcctca
     8161 ctcctttcaa tctctgttct taagttccgg tgaatgggga aagaatccag ttggtaatcg
     8221 acacctttga agagaacttc caaaaaactt gtgccggtat tatgctcgta ttcctcacgt
     8281 aggtttttat ccgaaatcgg aacgacgtga taggctttga ggttttcatc catgaggtaa
     8341 ctggagccgt gtttgtcaaa aagagttttg agagtggctc cacggaccat atccaaaacc
     8401 gattttgcat ttggtgcggg ggcttctctt tcattgagtc ttaataaata gagaagtttg
     8461 cgttgagtgc tgcccatgcc ccaacaggcg gcgagtttga aactgccact atggaagccc
     8521 caatttttaa atggcaggtg attgacggaa gaaaaggcct cttgacggat ccttataaga
     8581 atagtgccga tgctgagtga gttgcaactc aagttgttta ctaaagagtc cgagatcacc
     8641 ttggtgttgg cgaaagagat atttcccccc agaataattg ctagtgctgt catcgcaaaa
     8701 aactttttca tatttccctt tcttttatcg ggtacgtatg tatttttttt gaagagagat
     8761 ctcctttatt cggtcttccg gaaaatttct aaaatgcaat tgggcctatg ctgaagcgga
     8821 atatgttaaa cgaaatttta ttccgtcatc tattccgtta ctttgagtcc ttcggaccag
     8881 aacctggcgg attcaatgag acctgacagt accgaatgga aatgggattg ggcttgcaag
     8941 cgttcgcgtc cgaatctttt ttcaagttct ttttggatga agcctacttt ggctgtgcca
     9001 ccggtcaaac aaacgagatc cacttgttga gaagtcaagc ccgcgctact gagacagcgg
     9061 tccagtcctg caaagatagc ttccttagta ctttccgccc aatcaacaaa ttgtgattga
     9121 gagaagcttt cgtagatttc aattccggga taggcaaatt tgaagctgct aaggggatga
     9181 tcggagagtt ctcttttggt tttttcaatg gtttcaaaaa aaggaaaaat ttgttggtct
     9241 tcaataagga cgaagagtct ttcaatggcg gccttgtctt tttcggtgat cgcgcatttt
     9301 cctatttcgc ggataaattc ataggtttct ttttctttaa ggtggacaat gtgcgcggga
     9361 tgattcaatc gttgagtcac gcctgcgggc atggtcagaa cattgctgga taaagggaag
     9421 cgatagcggg atttggctcc gaaatattcg ttgagacgat gactcataaa aatgctgtcc
     9481 aaggcgtctc cggcgagggg gcagccatca atggaaagga cgtcttcacg acggaatttt
     9541 tccgggctta agcgaattaa agtaaaatcg gaggttccgc ctccaaagtc tccgatgaga
     9601 acgatttttt cagtggtcaa ttggcggcga taatcaaaag cggcggctaa aggctctgga
     9661 acaaattgaa tatttttaaa acctgcaaac tgtgtggcct tttgcatgcg gtgaagggca
     9721 aagccatcgg ccacaggatc catggagtag cgtgcaggac ggccaatgac cgcgttttca
     9781 atcgggcatc cgagaagagc ttcggcgcgt tttttgagtt ccagaaggaa aatccccacg
     9841 agattctcga gggtcagaat ccgattgctg aggatcgttc ctaaataagc ttgattggga
     9901 aggtgagact tgaaggagcg aaaaagacgt ccttccatat cctgttcaat atactgttca
     9961 atggcttcgg ccccatagta acaaagatcc gcatgcggaa agtaaagcag ggtgcgcatc
    10021 aaggtggggt cggtggcttt tgggtctagc ttgagagctt caatccgctg atttttatga
    10081 aaggctccga cgagggagtt gctcgtacca aaatcgatgg ctagaaatgt ctctgacata
    10141 cgtattcagg ataaggacac ctggaatcag agacaatttt ttaatcagaa tctagccctg
    10201 aacattagtt gtgttttttg tatgctcgac catcatgtct tctgcacgct gaaggtctgc
    10261 gggcgtgatt tgccaaattt gtttttcttt aaattctttc aaaagaagat tgaaaagatc
    10321 aaactttaaa aaaggcgtga ccgcgccgct ggcgtattct ctttcttttt caccataaag
    10381 gcccagaaaa gttttatcac ggaaccaaga ttcacgattc atactttgag cggttttcac
    10441 atgttcttca ctgggattgc gctgcaagaa ttcaaagaac attccgctcg gctttaggcc
    10501 ggggagatac cattcaccac tgaaaacctg gatcaggtct tcctcttcat ctttcagaat
    10561 gggagtgatg aattgcactc cgcgttccac ggcatgttga tggaaagcca aaaggtctgg
    10621 agtgcgaaga gctatatgtt gccaatgggc agactgctga tgcccatcca acatctcgcg
    10681 aacgtgagaa ctttgagttt taggttccgt aggttgtacc accgcaataa tggtattgtt
    10741 taactcagga tcggggttgg cgccgcggcc aaggcaaatg gcataggtca tggatttttc
    10801 gccttcaccg ggaacccact ctttgcgttt gtcataaaga agatcttcag gacccactcc
    10861 aaaaacagta tggaaagcca aataggaaac tttatataaa tccggttgaa gaagaagggt
    10921 catgtgatca acttgaaaat tcagaaaaga tttttttgcc atgttcatcg ctcctgcgta
    10981 agtgaaatac gcaggagatc atcgtctgtg cttttgtgaa tttcaaggga tattatttgc
    11041 agtaaggcgg atcgagcagt ggagcacgca tcgcttcggc aaccagtcca ttcatatcgg
    11101 ctctcacgtc gaaatgattc caatccgtgc acttgatttg agtggcgttg gcttgagagc
    11161 gcatcataga gctattgagg ttttctaagc cgtaaaattg ctccaccgtt ttttcatctg
    11221 ctttaattaa acgctgaaag acgccttgtg caaacggaga ttcttctcct tttcgggtca
    11281 agtaaatgat cttctcgcaa ccataggctt tcagcaccat gaccggatgt agatcactcc
    11341 agccaccggc ggaaagctca gttctggaga gggccacgat atgggaaagt cccggctctg
    11401 caggagaggt cgaaaggacg ttcaaccaag gggcttgtcc taaagaaagg aatttttgag
    11461 acttggcatc gtccttatac tcagcagcgg ttctgatttt attttcaatg gcgttgagat
    11521 cggcaggttt tccccagtag ccgaagcgaa gctcctcggg gttcaccgta aaatccccga
    11581 agtttggatc tggattggtt tggtaatcga cataaagctg agtgaattgt tccacggctt
    11641 ttccgtaaag aacagaagtc gtcggaaaag aaggaatgtg gctgccaaca aggtcattga
    11701 cgcggctttg gctcgagttt ttcgcttcac gatacataag aattgcaccg cccaggagtt
    11761 ggcggcaggc tggacgttgt tggttcaatt cagcccaagt catattcttg gattcagggg
    11821 tgcaaagatc taaaaaatta cggaccagtt gttgaacttg ctttccagtg gcggattgca
    11881 ggttgtagcc ggcgtagaaa tcagccattt tacctaggat gcgaccgaga cttgcgaaat
    11941 taataagccc tggtctaaga aagagagtct tatcagtctt agcatcgaat ttaccgaaat
    12001 ttttgaaagc ctgtttgatt tgattcacgc gataattcac gatgggtttg agtgagtcct
    12061 cttgtaactt ggccatggcg agagttcttt tcacatagag aataaattct ggattgagaa
    12121 tctccttaag atcttcggag gccataaggg cttgtaaaga gcccaggtgt tttaccagaa
    12181 gagctttatt cgcaggattg gccaaagcct catcgagttt cggtgaagtc gcaatgatac
    12241 tttgaaaagc gcttttgtct gataagaggc tttgaatggc tttaccttca ggagtttgcg
    12301 acagcgcttc taaatagcct tgaaatgatt taataagcag agctttttcg ctattattct
    12361 gagggagggg gttcaatgaa atgctttcta ataaaaacat agtgatactt gcagagcttc
    12421 cgccagccat cgccgcgggc aagccttgct cttcaacgat gcttgacata gctccccaat
    12481 gggcgggcat aagttcgcca tttccacgga tggcggcgca gtagttggcg taggctccgg
    12541 aactgaccac actcatcagg gcgcttataa gcaagggaaa gaaagcagtg cgtttcatag
    12601 gggactcctt tgaagggctc attatgcgac atctatgcca atcttaactg cgaataaatt
    12661 aactatgagg agggatgagg tgctcaaata ctttacgaag tgtcgtgaaa ttcgtcgaac
    12721 tttttgacga cgagaatata taactccagg acatcagcgg cagattttat cttaagttta
    12781 ttctcaattt gcagggtttg tggagttgca aaagcctata aggttctgtt ggcgtattag
    12841 tttgagatgt ggcattctca gagaatgatt tatgtagatg ctgatggttg ccctgtaaaa
    12901 gatgagattt ataaagttgc tgagcgtttc caaatcgaag tgattctggt tgccaataag
    12961 actatgaata ttcccttcaa taagcttttt agaatggagg tcgtctcctc cggctttgat
    13021 gcggcggatg attggatcgt tgagcaggcg acacatcgtg atctcgtggt gacgggtgat
    13081 attcttttgg cggaacgttg tgtgaaaaaa gcctgccgtg tcctcggtca taaaggcata
    13141 gaatttaatg aagataatat cggtatgtct gttgccaatc gtgaattgat gcaaaactta
    13201 agacatatgg gagaagttcg tgggggtccc tcgccgatgg ataaaaatgc gcgctcatgt
    13261 tttctgagca atttagatca aattttgcat gcacttaaaa aagcccgata agtcaggtat
    13321 tcagcggcct ctcctgctca ggaagttttt gctcgggatg ttctggctca gagaaaaact
    13381 cgagtatctg atcttttctt tgcaaagtgt cttcatagcc taattctaaa agagaacgca
    13441 tatatctggg ttcaaaagac aaataactta acagatcctt actcttttct tcagtgaggc
    13501 caatgccttc cagcagaaag cgcaaagtaa aaggaatact ggaaagagcg caggccggca
    13561 tattgcccac ctcttcagag ggtctcaaac tgaggacagg aatatttcgc aaatgatccg
    13621 gctctcgttg cagttcacta ggcgtcatca ccgagagcgt gcgattgatc cgcgcaatgc
    13681 gagcaagatc ggcatccagc gagtcaaaaa ataagccgtt caaaatggcg ctggcaattt
    13741 cactgaggga aatagtttct ttggtttgcg gattcgctgg agtggtggag ctggggccgc
    13801 gaatgccaat cactaaaaga ctctccgcgc ccatacgaat ggcggcgctg aggggagcgt
    13861 tgagacgaat catgccatcg ccatagaagg catcattgat tcgcaccgga ggaaagaaaa
    13921 cagggatagc ggctgaggcc atgacgtggt gagcagccag atcggaacga cgactgatac
    13981 gatttaaact ctgccaatct tgaatttgcg cactgcctga aaaaaaggtg gtggagtggc
    14041 cggtatggta gtttgccgct gttactgaaa cgccgtgaat tttctcggct cgaaggtaag
    14101 aattcaaagc atcgaagtta attttagatc taacgaagtg attgagtggg gccgtatcta
    14161 agagataagt gatctgctgt ttttttccca ttcctccttt gctgcggtct ttgatccagc
    14221 gaagaccaat attcatgagt ggaagaggtg cggtcttaaa gatctgttcc gtggtgagcg
    14281 aagcccagcc ttcccacaga ttccgggttg cttgctcaaa ttcctcatgg cgactggcga
    14341 tccaagtgcc attgatagcg cctgcggaaa agcccgaaat aatttgaaaa ggatttgttt
    14401 tcactcgaat gatgtcggag attccccgca gaactccgac ttgataagcg gcgcgtgcgc
    14461 ctccgcccgt gaggacgagg ccttttcgtc gttgtctggt gtctttaaat gtcgaaattg
    14521 tcatgcacac ctcagggcga taataacatg ctttgcgaat gcaactgatt tcattgagac
    14581 tgtaaatttg cagtgagaat ctaaaaaaca gagttatttg gcaagcctgg gtctagctag
    14641 agctaaagag caatccctcg gccatcaaca gagaaggggg ccagttttta actcaccatt
    14701 ctcagcaaga gcttcctttc gacgttaagc gagagagggc gattgatctg ttgacggcgg
    14761 tgcttctgcg cattttcaat taatttagca gccggttgca cagggggacg acccgggcgg
    14821 agtcctcgag aacttgacgc gggcttagtg ttggttaatt gggacattag catcgaagtc
    14881 cgcgccactg tattgaatat tgcaatagga tttttattag gagaaccggc cagttcttcg
    14941 cgagtcgcca gagcgctggc agccgcgcca atcatgaaaa tcggtaaggc ctctccgttt
    15001 tccgcgaggg cttggcgccc tagcagtgtg gttgttagat tcaattcagg aagggggcct
    15061 gtgatgtcgg cagtttttgc ctgcgctcct gcactgctta ggatctggga gactgtatct
    15121 tgatatccgg tggcatcaat aaccatatca cctacggttt caaaactttt acctgttgtt
    15181 ttgtcggtgg ttgtcaggtg catcttggag tcaggacgga gttcccagct ctgaacatgg
    15241 ccaggtattc ctttgatcat tccgcgttgg tagaagggaa cgatcttatg aatgtaacgg
    15301 tcaccatccc ggcttgcgat atattcttcg gctgtttgta ggctgtttcc gatccaagtt
    15361 atatggaacc cttccggcaa ttttacttga ggtgaattca agccttcgac gcaaatacgt
    15421 gagccatctc catcgccgac cata
//
LOCUS       DATBTE010000148         2128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_361258, whole genome shotgun sequence.
ACCESSION   DATBTE010000148 DATBTE010000000
VERSION     DATBTE010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361258"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VIG33_05635"
     CDS             complement(<1..939)
                     /locus_tag="VIG33_05635"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEY8270350.1"
                     /translation="MQEHYYKFWREMIKPKGFEVDRETLREDYAKFIIRPLERGFGVT
                     LGNSLRRILLSSMMGSAITAVKFDGVLHEFTTITDVLEDVTDIILNLKQVRFRQYTSD
                     SLTLKISKKGPGKVTAADIITSDKIEVLNPEEHIATLGANANFNAEIIVDFGRGYVPT
                     ENREKEFPVGFIGVDALYSPISKVNYSVSNARVGQRTDYDALALEVWTDGSLKPEEAV
                     ALSSKIMKEQLQIFLTFDETMEPSEEARDSSSPTLNENLFRSVDDLELSVRSANCLKN
                     ANIRYIGELVVRSEAEMLKTKNFGRKSLNEIKEILSE"
     gene            complement(958..1584)
                     /gene="rpsD"
                     /locus_tag="VIG33_05640"
     CDS             complement(958..1584)
                     /gene="rpsD"
                     /locus_tag="VIG33_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEY8270351.1"
                     /translation="MSCINQGVCKLCRRENVKLFLKGDRCYTDKCAFERRPYPPGQHG
                     QSRLKFSEFALQLREKQKTKRYYGVSESQFVKYVKEANRSKELTGTALLKNLEMRLDN
                     VVYTLGYANSRREARQLVKHNHFLLNGKRSNIPSILVEKGDVVQVAEASKEVVKIQSA
                     IQAVARRSVPSWLEADHANFKGVVKDSPVRDDIALQVEENMIVEYYSR"
     gene            complement(1596..1982)
                     /gene="rpsK"
                     /locus_tag="VIG33_05645"
     CDS             complement(1596..1982)
                     /gene="rpsK"
                     /locus_tag="VIG33_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEY8270352.1"
                     /translation="MEKKPVAKKKVKRNVPQGNCYIQAGFGNVIVTITDPSGDTVSWS
                     SAGHLGFKGSRKGTPFAAQVAAEDAAKKAMEAGMKSLDVYLKGPGAGREPAIRALAAT
                     GMRILSLKDVTPVPHNGCRPPKRRRI"
     gene            complement(2009..>2128)
                     /gene="rpsM"
                     /locus_tag="VIG33_05650"
     CDS             complement(2009..>2128)
                     /gene="rpsM"
                     /locus_tag="VIG33_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEY8270353.1"
                     /translation="CYRGIRHRKGLPVRGQNTRCNARTRKGPKKTVANKKKAV"
BASE COUNT          619 a          493 c          376 g          640 t
ORIGIN      
        1 ttcagacaaa atctctttaa tttcattaag agattttcta ccaaagttct tagtcttaag
       61 catttcagct tcagaacgta caaccaactc gccgatatag cgaatattag cattcttcaa
      121 gcagttagca ctacgaacag aaagttcgag atcgtcaact gatctgaaga gattttcatt
      181 caatgttgga gaagaagagt ctctagcttc ttcagaaggc tccatagttt catcaaaagt
      241 aaggaaaatt tggagctgtt ctttcataat tttagatgaa agagcaactg cctcttctgg
      301 tttcagagaa ccatcagtcc atacttcaag agctaacgca tcataatcag tcctttgacc
      361 aactcgagca ttagacacac tatagttaac tttgcttata gggctataaa gagcatctac
      421 gcctataaaa cctacaggaa attctttttc tctattctca gttggaacat acccacgccc
      481 gaaatctacg ataatctcag cattaaagtt cgcatttgca cccaaagtag caatatgctc
      541 ctctggattc aaaacttcta ttttatcaga ggtaatgata tcagcagcag taactttgcc
      601 tgggcctttt ttagaaatct tgagagttaa actgtcgctt gtgtactgac ggaaacgaac
      661 ctgcttaaga ttcaaaataa tgtcagtcac atcctcaagg acatctgtga tcgtagtaaa
      721 ctcatgaaga acaccatcaa acttaactgc agtaatggca gatcccatca ttgagctaag
      781 aagaattctc cttaaagaat taccaagagt gaccccaaaa cctctctcaa gggggcgaat
      841 aataaatttt gcataatctt ctcgaagagt ctcacgatcg acttcaaacc ctttgggttt
      901 aatcatctct ctccagaatt tataatagtg ctcttgcata gctcccccgc gatgtaatta
      961 tcttgagtag tattctacga tcatattttc ttcaacctga agtgctatat cgtctctcac
     1021 tggagagtct tttacaacac ctttgaagtt tgcatgatca gcttcaagcc atgaaggtac
     1081 agatcttctt gctactgctt gaattgcaga ttggatctta actacttcct tgctcgcctc
     1141 agcaacttgg accacatcac ctttttcaac aagaatactt ggaatattcg atctctttcc
     1201 attcaaaagg aagtgattgt gcttcacaag ctgtcttgct tcccggcggg aattcgcata
     1261 gccaagtgta taaactacgt tatctaatct catttcaagg ttcttaagga gcgcagtacc
     1321 agtcaactct tttgaacgat tggcttcttt aacgtactta acaaactgac tttcggaaac
     1381 gccataatat cttttagttt tttgcttttc gcgaagctgt aaggcgaatt cggaaaattt
     1441 caaccgagac tgtccgtgtt gtcctggagg ataagggcgt ctttcaaaag cacacttatc
     1501 tgtataacaa cgatcgcctt tgaggaacaa cttcacattt tcgcgacgac aaagtttaca
     1561 cacaccttga ttaatgcagc tcacgacaaa tctccttaga ttcttctacg cttaggtgga
     1621 cggcaaccgt tatgaggaac gggagtaacg tctttcaggg aaaggattct cataccagtg
     1681 gcagccaaag ccctgatagc tggctcacga ccagctccag gacctttaag gtaaacgtcc
     1741 aaagacttca taccagcttc catagccttt tttgcagcat cttcagcagc cacttgagcc
     1801 gcaaatggag tgcctttacg acttcctttg aaaccaagat gacctgcaga tgaccaagaa
     1861 actgtatcac cgcttgggtc agtaatagtc acgatcacat taccgaatcc agcttggata
     1921 taacaatttc cttgcggaac atttcttttt acttttttct tagcaacagg ctttttttct
     1981 atattattca ttgattagcc actcctaact atacggcttt tttcttattc gccacagttt
     2041 tcttaggtcc tttacgagta cgcgcattgc aacgagtatt ctgaccacga accggcaagc
     2101 ctttacgatg acgaatccct cgataaca
//
LOCUS       DATBTE010000149         2530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_361453, whole genome shotgun sequence.
ACCESSION   DATBTE010000149 DATBTE010000000
VERSION     DATBTE010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361453"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VIG33_05655"
     CDS             complement(<1..777)
                     /locus_tag="VIG33_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY8270354.1"
                     /translation="MEVRNRKTLIIGTGPAGLGAADYALSQQMDYEILESAPTPFGHC
                     KSFSLPSHPDIWFDEGPHISFTKNKQVQDIFAKSAPDFLNLKPLIENYWRETYIPHPV
                     QANLRSIPEPYLSEIVADIPRRPNIDNPTNYGDWLRTAFGDKFKSLFHEVYTQKYWTV
                     PSQQMTVDWIGERVYRPQAEAMLRTATTEDFKPSSHYITDFRYPRAGGFQSFLKKTMK
                     AHQEKTHFNSKVVQISPSKKQVITDDGRVWNYDVLLSSLPS"
     gene            878..1825
                     /locus_tag="VIG33_05660"
     CDS             878..1825
                     /locus_tag="VIG33_05660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8270355.1"
                     /translation="MVGVVIPCYKCKKHIVEVLAKIGSEVDKIYIIDDCCPERTGQFV
                     EQTVKDDRVQVIYNEVNLGVGGAVVAGYLRAQREGCTVVVKLDGDGQMDPSMIQRFIN
                     PILRGYADYTKGNRFNQIDYLADMPPIRIFGNAVLSFFTKLSSGYWTLFDPTNGYTAI
                     HTAILDQVGLEKLHKRYFFESDILFRLNICRAVVVDIPMPAKYGEEVSGLNELRIIPT
                     FIWGHVKNFCKRISYNYFLRNFSLASLQFIMGILLLFFGIVYGGFQWWHSYILEVTTP
                     SGKVMMAALPVILGIQFLLSFFGYDISSMPQFPLHKKIQ"
     gene            complement(1861..>2530)
                     /locus_tag="VIG33_05665"
     CDS             complement(1861..>2530)
                     /locus_tag="VIG33_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270356.1"
                     /translation="AKVKTYESEPSFVYAGGAQKWQCTEVMMESINSSHMNGTILSPD
                     KDYFSKILKRGKIKLKSTNSEGVVQELKGAHFSFVLRESNLINQVACPTKLIEAMTYG
                     CLPVLENTDLGDFKDLGYQYISLANFKDNKLPSSIEFEKAILKNKLIIDSLFEHYKIT
                     TDHIFSSFKETTTATNSQLEEIHKFLLLERARVLQKLVLSKRILAPREIYRFLRFLVK
                     KYLG"
BASE COUNT          665 a          466 c          577 g          822 t
ORIGIN      
        1 acttggcaat gagcttaaca atacatcata gttccaaact ctgccatcat cggtaataac
       61 ttgttttttg gagggactaa tctgaaccac cttcgagttg aagtgagttt tttcttggtg
      121 agctttcata gtctttttta aaaaggactg aaaaccccca gcgcgagggt agcgaaaatc
      181 agtaatataa tgacttgaag gtttgaaatc ttcggttgtc gccgttctga gcatcgcttc
      241 ggcctgcggt cggtaaactc tttcgccaat ccagtccacg gtcatttgtt gagagggaac
      301 cgtccaatat ttttgtgtat aaacctcatg gaataaagat ttaaatttat cgccaaaggc
      361 cgttctcagc caatcgccgt agttggtggg gttgtcaatg ttgggtcgtc ttgggatgtc
      421 agctacaatt tcagagaggt aaggttctgg aattgatctc aagtttgcct gaacgggatg
      481 aggaatgtaa gtctctctcc aataattttc gatgaggggt tttagattca agaagtcggg
      541 ggcgcttttt gcaaagatat cttgaacttg cttgttctta gtgaagctta tgtgaggacc
      601 ctcatcgaac caaatatcag ggtggcttgg gagggaaaaa cttttgcagt gtccaaaggg
      661 tgtcggcgcg gattccagga tctcgtaatc catttgttga gataatgcgt agtctgctgc
      721 tccgagtcct gcgggccctg ttccaatgat aagggtcttg cgattgcgta cttccattta
      781 ctctcttctc cccagcgtcg cagtgatatt aatagtgctt attttggcag atcaagataa
      841 aattgtcttt tgtggattat gtttaagatg aggttttatg gtcggcgtgg ttataccttg
      901 ctacaaatgt aagaaacata ttgttgaagt cctcgctaaa attggttctg aggtcgataa
      961 aatatatatc attgatgatt gctgccctga gcgcactggg cagtttgtcg agcagaccgt
     1021 gaaggacgac cgtgttcagg ttatttataa cgaagttaat ttaggagtcg ggggggcggt
     1081 ggtagcgggg tatcttcggg cccaacgcga ggggtgcacc gttgttgtta agttggacgg
     1141 ggacgggcaa atggacccat ctatgattca gcgatttata aatcctatcc tgcgaggtta
     1201 cgcagactat actaaaggaa atcgatttaa ccagattgat tatttggctg atatgcctcc
     1261 cattagaata ttcggcaatg ccgtcttatc tttttttacc aagctgagtt ccggctattg
     1321 gactttgttt gatcctacca atggctatac tgcaattcat acagccattc ttgatcaagt
     1381 gggtttggaa aaactccata aacgttattt tttcgaatcg gatattttgt tcaggttaaa
     1441 tatttgccgg gccgtggttg tggacattcc tatgccagca aaatacggcg aggaagtgtc
     1501 gggccttaat gaactgcgga ttatacctac atttatttgg ggccatgtga aaaacttttg
     1561 caaacgtatt agttataatt atttccttag aaatttcagc ttagcgtctt tacagtttat
     1621 tatgggaatc ttgctgctct tttttggtat tgtctatggg ggattccaat ggtggcacag
     1681 ttatattcta gaagtgacta ctcctagcgg aaaagtaatg atggcggccc ttccggttat
     1741 actgggaatt cagtttcttt tgagcttttt tggatatgat atttcctcaa tgccacaatt
     1801 tcctctgcat aaaaaaattc agtaagaaac agctcttctt cgcaaggaaa ttggatgatt
     1861 ttaacctaaa tatttcttaa ccagaaatct gagaaatcgg taaatttctc gaggtgcaag
     1921 aatacgttta ctgagaacga gtttttgcag aactctggct ctttccagca aaaggaactt
     1981 atgtatttct tcaagctgtg aatttgttgc cgttgtcgtt tctttaaatg aagaaaaaat
     2041 atgatctgtt gttattttat aatgttcaaa aaggctatct ataatcaatt tgttcttcaa
     2101 aatagccttt tcgaattcaa tagaggaagg aagtttgttg tctttgaaat ttgccaatga
     2161 aatatattga tagcccaaat ctttgaagtc ccccagatca gtgttttcta aaacaggcaa
     2221 acagccatag gtcatggctt caatgagttt cgtagggcaa gcgacttggt taataagatt
     2281 agattctctc aagacaaaag aaaaatgggc ccctttaagt tcttgcacca ctccttctga
     2341 attggtggat tttaatttta tcttgccgcg tttaagtatt tttgaaaaat agtctttgtc
     2401 aggcgataag atagtgccgt tcatgtgaga actattgatg gactccatca ttacttccgt
     2461 gcactgccac ttttgagcgc caccggcgta cacaaaggac ggctccgatt cgtaggtttt
     2521 aacttttgcc
//
LOCUS       DATBTE010000150         4658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_292424, whole genome shotgun sequence.
ACCESSION   DATBTE010000150 DATBTE010000000
VERSION     DATBTE010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292424"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VIG33_05670"
     CDS             <1..764
                     /locus_tag="VIG33_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270357.1"
                     /translation="LGYFDDSNGEEKIWNGINYGLSPSLDIEIFHALRKELTAPCQGA
                     TLRLCGFTQSGEPKSGKVILQKFINLHGKKVLVNLTLTQASASPYFLQNKGDLASRQA
                     FLTQQSEENFFQGLKTADVLFYNGHSRNGGGPDFAPPVLDSTNHVNYKGYYKVKRAGI
                     QKTIAAMKQNLHQQPLVGLFSCYSRSHFYKTFMRLNPNQALILSADTINYFDSMKASV
                     GYLEGILRGTCSQELADTAKQEAQIKTGFQGYNIK"
     gene            complement(1317..1601)
                     /locus_tag="VIG33_05675"
     CDS             complement(1317..1601)
                     /locus_tag="VIG33_05675"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8270358.1"
                     /translation="MEMFWEFDGWELQDKHLKQMQSSPFVRPFPALKIEPGLFVLRGP
                     RQVGKSTWLKTILAAKSNRKTCFYLSCENVRDQIDLSEILKSVRDRQIIL"
     gene            1763..1936
                     /locus_tag="VIG33_05680"
     CDS             1763..1936
                     /locus_tag="VIG33_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270359.1"
                     /translation="MFSLIARVFGGLVDAFSATWTEFHRGIGTTMVHVGTAGFLIHTS
                     EDMEVEVIDLFSL"
     gene            complement(2016..2294)
                     /locus_tag="VIG33_05685"
     CDS             complement(2016..2294)
                     /locus_tag="VIG33_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY8270360.1"
                     /translation="MGKKIYVGNLSFNVDSNQLQDVFAEFGTVDSTNVITDRETGRSK
                     GFAFVEMAAEADAQNAIQQLNGMDLSGRAMNVSEAKPQEPRTGGRSRY"
     gene            complement(2433..3782)
                     /locus_tag="VIG33_05690"
     CDS             complement(2433..3782)
                     /locus_tag="VIG33_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY8270361.1"
                     /translation="MSRFKFLTESRKTLKLAVPIIIGNVGHTIITLTDNIMVGRLGAN
                     SLGAAGFSGSLFYVFLLFGVGLLAPTAALFAQSHSREDHHEGGELLRHAVFAATLVSL
                     ILMLFLWFTRPFMAYMGQTAEVVELSKSFSSFLIASLLPSLLYQAYKQFTDGIGKTTI
                     GMYVMIAAILINIIGNYIFIHGLWGMPALGLNGSGLATLLSRSFMALVMMIYIHRHPE
                     FQKYFAHRWRRLIHQGQLKKIFYLGLPNGFTYVFEVGAFSFSSIMMGWLGAAQLAAHQ
                     IALNLASISFLVTVGIGAAGTIRVSSEIGLKHPDKARYAGRVAMTLGAVYMLFSAFLV
                     FNARQFLASIYTYDLQVIAFASTYLVVAAAFQFFDGIQAVAIGILRGFQDTTWPSVLA
                     LVAYWIIGLPIGYSLCFHLGLAGRGIWWGLFIGLLLIAIFLALRFERLSQRYISKKD"
     gene            complement(3769..4350)
                     /locus_tag="VIG33_05695"
     CDS             complement(3769..4350)
                     /locus_tag="VIG33_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEY8270362.1"
                     /translation="MANTNNNPSNKVTFESTPNPATYKFLFHRQVTEEALDCPTAQEA
                     ERSPLAAKLFGFPWTSSVFIGQDFLTVTKQDWVDWNMLAEPLTGLIQEHLDKGEPVVH
                     KAQTAQEVSAEGFTNDTPLVKQIKNLLDVEIRPVVALDGGDIIFNRYENNILYIHMRG
                     ACAGCPSSTATLKEGIEVRMKEAFPEIHEVVAI"
BASE COUNT         1341 a         1008 c         1042 g         1267 t
ORIGIN      
        1 ccttggggta ttttgatgat tcaaatggtg aagagaaaat ttggaatggc ataaattatg
       61 gactctctcc gagcttggat atcgaaatat tccacgccct tcgtaaggaa ctgactgcgc
      121 cttgccaagg ggctacgctg cgcctttgtg gctttacgca aagtggggag cccaagtctg
      181 gcaaggtgat tctgcaaaag tttatcaatc ttcacggtaa aaaagttttg gtcaatttaa
      241 cgctcaccca ggcttctgca agtccctatt ttttgcaaaa taagggagat ctggccagcc
      301 gccaagcatt cttgacccaa caatctgaag aaaatttttt tcagggcctg aaaaccgcag
      361 acgtgctttt ttataatggt cactctcgta acggaggagg tcctgatttt gccccccctg
      421 ttttggattc cacaaatcat gtgaattaca agggctatta taaagtgaaa cgtgcgggca
      481 ttcagaaaac tattgccgct atgaaacaga atttacatca acaacctttg gtgggacttt
      541 tttcttgtta ctcaagaagc catttctaca agacctttat gcgcttaaat ccaaaccaag
      601 ctttgatact aagcgcagac actatcaact attttgattc catgaaagcc tcggtgggat
      661 atttagaagg tattttacgg ggaacttgca gccaagaatt agctgacact gccaagcaag
      721 aagcacagat aaaaaccggt tttcagggct ataatatcaa gtaggtaaga ggatcattat
      781 ttggtggaag ttcttttaaa aaatttgtct gtgaccaaat ggatttgaac ggcgtcctta
      841 gttgtttata ggtttctgaa gcgttcttta caacaggttg ccatttgact tctatgccga
      901 atgcggaatg tttgtaaaaa tcaacttccc catttttatt tcgaatatac cccaatcttt
      961 taaatttcct atttaaatac tcgaaggcaa ctgattctgc caatttatcg aaggcttgtt
     1021 cgctaggctt ttcgtcgagc caatctaaaa ccatccaata aattaatgga tctctaaagt
     1081 agaatttctt ttctttttta aaatgatagg atcttgatgt ctcatttgtt gaaattcaaa
     1141 aaagtccatt ggtaaaagag tgatttcgcc ttcgggatga acacggcctg gcatttgatc
     1201 agcgccgtgt cttaggtctg acgtgttgga tccggtgaga attaaagttg cactaattcc
     1261 gcgatctact tcgtgcttaa cggctcgatc ccatcctttt acaaaagata cttcgtctaa
     1321 agaataattt gacgatctcg aactgacttt aagatttcac tgagatcgat ttgatcgcgc
     1381 acattttcac aactcaaata gaaacatgtt tttctgttgc ttttcgcggc aagtattgtt
     1441 ttcaaccacg tgcttttgcc cacttgtcgc gggcctctta atacaaaaag accgggttca
     1501 atttttaatg ccggaaaggg tcttacaaac ggcgatgatt gcatctgttt taggtgttta
     1561 tcttgaagct cccatccgtc aaattcccaa aacatttcca tagtttatct cataaatata
     1621 tgattaggca gtctacctta tatttttggg cagtaaatat taaaaatttg ggcagtgatt
     1681 ttcagggtca gggagcttct tatttaacta atttctttcc acagtcatgg cagaagaggt
     1741 ttcgttcttt acggcgagca gcgtgtttag tctcatcgca agggtttttg gcggccttgt
     1801 tgacgctttc tccgcaactt ggacagaatt tcaccggggc attggtacga ctatggttca
     1861 tgttgggaca gcgggctttt tgattcatac gagtgaagac atggaggttg aggtcataga
     1921 tcttttctcg ctttagattg aatttttaat aacgaatgaa agcaaagaag ccgacccgag
     1981 agtcagcttc ttgtgcattc taaaatttta aaaacttagt aacgtgagcg gccaccagtg
     2041 cgcggctctt gaggctttgc ttcagaaaca ttcatggcgc gacctgaaag gtccatgccg
     2101 ttcaattgtt gaatcgcgtt ttgggcatca gcttcggcgg ccatttcaac aaaagcaaaa
     2161 cctttgctgc ggcctgtttc gcggtctgtg atgacatttg tggagtcgac agtgccgaac
     2221 tcagcgaaaa catcctgaag ttgatttgag tcaacattga aagaaagatt acctacgtag
     2281 atttttttac ccatatttgc ctcctaaaaa agcttggggg ccattcgcag aagacaaaag
     2341 gacatctaat gaactcgaga acggacataa aaagacttaa cttcaaacat tagaccacca
     2401 atcggtcggc atagataggt atatgtgctt ctttagtctt ttttactgat atatctctga
     2461 ctcagtctct caaatcttaa agccaagaaa atggcaatta acaaaagacc tatgaacagg
     2521 ccccaccaaa tacctcgacc tgcaagaccc aaatgaaagc acagagagta accaatgggg
     2581 aggccgataa tccaataggc gaccaaagcc aagactgacg gccaagtagt atcttggaac
     2641 ccacgcaaaa ttccgatcgc cacggcctgt attccatcaa agaattgaaa agcggcagcc
     2701 acaactaaat aagtcgaagc aaaggcaatg acttgcaagt cataggtgta aatgctcgct
     2761 aaaaattgac gagcattaaa cacaaggaag gcgctgaaca acatatagac agctcccaaa
     2821 gtcatagcga cgcgacccgc atagcgcgcc ttatcagggt gtttaagtcc gatctcggaa
     2881 ctcacgcgga tggtgcctgc agcgccaatt cctaccgtga ctaagaaact gatcgaggcc
     2941 aaattgagtg caatctgatg ggcggccagc tgtgccgccc ctaaccatcc catcatgatg
     3001 gaggaaaaag agaacgcgcc cacttcaaaa acataggtga agccgttcgg aaggccaaga
     3061 tagaatattt tttttagctg tccttgatgg atgagtctgc gccaacgatg ggcgaaatat
     3121 ttttggaatt caggatgtcg atggatatag atcatcatca ccaaggccat gaaactgcga
     3181 ctgagcagtg ttgccaggcc agagccgttt aaacccaagg caggcattcc ccaaagccca
     3241 tggataaaaa tatagtttcc tatgatgttg attaaaatgg cggcgatcat cacgtacatg
     3301 ccgatggtgg ttttgccaat gccatcagta aattgtttgt aggcttgata cagaaggctt
     3361 ggcagaagtg aagcgattaa aaaagaagaa aaacttttag agagctccac gacttccgct
     3421 gtttgtccca tataggccat aaaaggccta gtgaaccaaa gaaagagcat cagtatcagt
     3481 gaaacaaggg tcgctgcgaa aaccgcatgg cgcagaagct ctccaccttc gtggtgatcc
     3541 tcacggctgt gggactgagc aaaaagcgca gcggtcggag ctaacaggcc cacaccaaat
     3601 aaaagaaaaa cgtaaaagag gctgcccgag aaccccgccg cacccaagga attggctcca
     3661 aggcggccca ccataatatt gtcggtgagc gtaataatgg tatgccccac gttgccaata
     3721 ataataggaa ccgcaagttt cagggttttg cggctctctg tgagaaactt aaatcgcgac
     3781 aacttcgtga atttcaggaa aggcttcttt catgcgaact tcaatgcctt cttttaaagt
     3841 ggctgttgag ctggggcagc ctgcacaagc tccacgcata tgaatgtaaa gaatattatt
     3901 ttcgtatcta ttgaagataa tatctccgcc atccagggcg accacggggc gaatttcaac
     3961 atcgagcaga tttttgattt gttttaccag cggagtatcg ttggtaaatc cttcagcaga
     4021 aacctcttgg gctgtttggg ccttgtgcac cacaggctcg cctttatcca agtgctcttg
     4081 gataagcccg gttaaaggct ccgccaacat attccagtcg acccaatctt gttttgttac
     4141 ggtgagaaaa tcttggccta taaaaacaga gctagtccaa gggaatccaa aaagctttgc
     4201 tgctaaaggg gagcgctccg cctcctgagc cgtcgggcaa tctaaagcct cctcggtcac
     4261 ctgacggtga aagagaaatt tataagtggc tggattcggc gtactttcaa aggtgacttt
     4321 attactgggg ttattatttg tgtttgccat agagataggc tcctttttga ggcagtgcca
     4381 acatacatta tagcagactt tctgccaagc gtgttaagtc catttttaat tgtcaaattc
     4441 ttgattaaaa aaggtaaaat gactaccttt tcggttcatt cacaaacagg agctaatgat
     4501 gacaccaaga gtaagagaga ttttaagctg gtacggcgct gacaacccgg gagtattgac
     4561 caacctggcg cgcatgatga atcacggtaa gcttgcaggc acaggaaaac ttgtgattct
     4621 gcccgtagat caggggtttg aacatggccc ggcccgca
//
LOCUS       DATBTE010000151         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1590563, whole genome shotgun sequence.
ACCESSION   DATBTE010000151 DATBTE010000000
VERSION     DATBTE010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590563"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="VIG33_05700"
     CDS             complement(<1..1243)
                     /locus_tag="VIG33_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8270363.1"
                     /translation="MIVSFSVANFRSFLGEETFSLVASSRVSGNHEGHAFFIPDSKEK
                     VLKTAVLYGANGAGKSNLFKALSYLKTIALKPRKKNSGTGRETFRFSAESSESSSFDL
                     QFIAGNKLYRFGFTLDDQRIIEEWLVRVKGGREIPVYERITDENGKVTIVAKGKGRKL
                     QALATVGGPQNQSFLATIQATLDVSDFGKELTEVLVWFNSSLTLIAPDASFARLGHLL
                     DSDSNFRTFASEFLKSSSTGVDHLQVQRSEITEDELRSLLPKRIVSKVLDDVRDEEDD
                     TVLLQLGEGNELLIERADENHFYRIRIQAAHEHETGNKVSLDLTEESDGTRRLLNLIP
                     ALHQLRTNSGVFFIDEIDRSMHPILVREFLDFFLKSCDGGQRQIIVTTHESNLLDLEL
                     LRRDEIWFAEKDVNAVTHLYPL"
BASE COUNT          450 a          373 c          284 g          453 t
ORIGIN      
        1 tcaatggata cagatgcgta acagcattta cgtctttctc agcaaaccat atctcgtcac
       61 gacgcaacag ttccaaatcc aataagtttg attcgtgggt cgtcacgata atttgacgct
      121 gaccgccgtc acaagacttt agaaaaaagt ccaagaattc cctaaccaat atagggtgca
      181 tgctacgatc aatctcatca atgaaaaaca caccactatt ggttcgcaac tgatgcaaag
      241 caggaatcaa atttaagagt cgtcgtgtgc catcagattc ttcggttaga tctagcgaaa
      301 ctttattccc tgtttcgtgt tcatgggcgg cctgaatcct gatgcgataa aagtggtttt
      361 catcagcgcg ttcaatcagt aattcattgc cctcacctag ctgaagtaat actgtgtcgt
      421 cttcttcatc acggacatca tccagtactt tagacacaat acgttttggt aataaactgc
      481 gtagctcatc ttcggtaatt tcgcttctct gcacttgcaa atgatccacc cctgtggatg
      541 aggatttgag aaactcactt gcaaacgtcc taaaattcga atcactatcc aacaaatgcc
      601 caagacgagc aaaagaggca tcaggagcaa tcaatgtcaa actgctattg aaccaaacta
      661 aaacttctgt aagttcttta ccaaaatctg acacatccag agtggcttga atagtcgcca
      721 gaaatgattg attttgaggt ccgccaacgg tggcgagagc ttgcaatttt cttccttttc
      781 ctttggctac gatggttact tttccatttt catcggtaat gcgctcataa acaggtattt
      841 cgcgtccacc ttttacccgc accagccact cttcgataat ccgttggtca tccagtgtaa
      901 atccgaatcg gtagagttta ttgcctgcga tgaattgcaa atcaaaactg gaagattcac
      961 tactttctgc gctaaagcga aacgtttctc gtcctgttcc gctgtttttt ttgcgaggct
     1021 ttaatgcaat cgttttaaga tagctaagtg ctttaaagag attggattta cctgcaccgt
     1081 tagccccata aagaaccgct gttttgagta ctttttcttt agaatcagga ataaaaaaag
     1141 catgcccttc atggtttcca gacactcgac tactggcaac aagagaaaaa gtctcttctc
     1201 ctagaaatga gcgaaaattt gctactgaaa aagatacaat catctgctat accgtgaatt
     1261 aatcgctaat atgctttatg ctataaaata tttgatgttt tatagcataa agacgtgatt
     1321 ttttcacacg tttaataatt tagatgtttt tgttgcggat gtagaatgag ccaaggatag
     1381 ctggctcatc tttatgttgc agccccgtgt agcgtataga atggtaaatt cccatcttca
     1441 atacccccca acaaccaaaa cactactccg ccccccgccc tgcttttcaa ccaccacctt
     1501 agccccaatc cgctccacca aaatcggcac atgcgagata acgccgactt tcctgcccaa
//
LOCUS       DATBTE010000152        20634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1386074, whole genome shotgun sequence.
ACCESSION   DATBTE010000152 DATBTE010000000
VERSION     DATBTE010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 20634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20634
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1386074"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VIG33_05705"
     CDS             complement(<1..197)
                     /locus_tag="VIG33_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270364.1"
                     /translation="MLIFETRDPQERYHLQVHRFIEGFHEAPDSFLSEEELQYFHHIT
                     NDKRKSEYLQSRFVLKSWLSKR"
     gene            complement(199..1494)
                     /gene="hmgA"
                     /locus_tag="VIG33_05710"
     CDS             complement(199..1494)
                     /gene="hmgA"
                     /locus_tag="VIG33_05710"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEY8270365.1"
                     /translation="MNKERDTKYFSGFGNHFSSEARPQALPLNQNSPQNCPLGLYPEQ
                     LNGSAFTAPRSTNLASWFYRIRPSVMHRPFVPLKELTERTLFKPKHINPNQMRWNPLP
                     HPTTPQDFLEGLRTVCGVGSAIEQRGLHIHLYAFNQAMGSRYMMNADGDYLFVLQLGS
                     IEIKTEYGIIEAEPGEIVVVPRGIKFQVNPQAAVCRGYVGENFGAPFRLPDLGPIGAN
                     GLAHGRHFMVPHAAFEDRDGKFELIGKFSGHLWKAEMNHSPLDVVAWHGNYVPYKYDL
                     RKFNTINTVSFDHPDPSIFTVLTSPSDTAGVANVDFVIFPPRWMVAEHTFRPPYFHRN
                     CMSEYMGLIHGVYDAKTSGGFVPGGGSLHNFYSAHGPDSDTYEKATNAELKPHKLENT
                     LAFMFECRTPYLLTDFAMQKDFLQEDYQECWQGFQKHFK"
     gene            1637..2065
                     /locus_tag="VIG33_05715"
     CDS             1637..2065
                     /locus_tag="VIG33_05715"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY8270366.1"
                     /translation="MKSLLTKYSNQLKKNGCYSWTIFFIRLVVGLAFMMHGGGKIQNS
                     FNWMGSDSSVPGIFQGLAALSEFGGGLAWILGLLTPLASLGIFFTMAVATLSHATRGD
                     SFVAHGAPTYELALVYLTIALFMMCNGPGKISADYKIFSS"
     gene            2128..2517
                     /locus_tag="VIG33_05720"
     CDS             2128..2517
                     /locus_tag="VIG33_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEY8270367.1"
                     /translation="MKLFLAFLFCFLGFSKAYAEESTSSVYECLFQNNFVTTQSLRMK
                     DYFDIEKGQRAGKVDLLENLETIESTPTLVYQIPFLDQQMYVQIWHSPTVRVDSQMSL
                     SGSNQTFSATYHKGSSQEELLCTTLGK"
     gene            2633..4159
                     /locus_tag="VIG33_05725"
     CDS             2633..4159
                     /locus_tag="VIG33_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEY8270368.1"
                     /translation="MKTFLLNPLFIVVLFITSSSWAGSLVNVSTLNKTLQKQKATWVA
                     KENWLTQLSKSEAKRMMGLRKTPPLDVQFVVPLSMTQDTLPPSLDWRNKDGRNWVSPI
                     LNQGNCGSCVAFAAIGVMETQLNISSLLPNLNIRLSPQHLFSCGDGACNYGWMPESAA
                     SQLVRTGVTDEACMPYTSGASGKDVACQASCSDLSQRIYKISDYNSPTESTMDVDAIK
                     RALQKGPLMTSLNVYADFMSYAGGVYKHVSGDYLGGHAISIVGYDDVSQSFIIRNSWG
                     DSWGEEGFGHISYEDTSGVGEATWGFDIPTIGGAISLTTPRDYTYMTGTVSLSAESTF
                     NNTDGISYAIYGSNSQVIWSGVCQGPNCSVSLDSSKFQDGRYEIQALATNAHGDNLGA
                     STKQFFYIVNQTPELKLSFTGLGRVDLSKPLKGRIEFSISAQSSGVPMSSLEFHYKDP
                     SGKETVRVSNVVLDQMTTGWRTNLIPNGRYEIWMIGRLQSNSFGLSIETSHSIVTIQN
                     "
     gene            complement(4231..4737)
                     /locus_tag="VIG33_05730"
     CDS             complement(4231..4737)
                     /locus_tag="VIG33_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8270369.1"
                     /translation="MNPSMSSSHDNVLQNVSLFQDIKHLPEALLSLRRIMKPCKYSKG
                     HVLITQGEAGSEFFVLLKGQVSIEKITPEGDRYKVAVLKGEQNAAFGEGGLIEGEARS
                     ATVTCDTEVECLVLSCKEFDQFSLAQPQFALPVLKRISLILMSRLNQTSNDLMLLHKA
                     LMSEIRSS"
     gene            complement(4718..5929)
                     /locus_tag="VIG33_05735"
     CDS             complement(4718..5929)
                     /locus_tag="VIG33_05735"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEY8270370.1"
                     /translation="MKKVKKILLRSKAFLFAQPFLVASTFFLAVWLGNAFIVYLFEQG
                     VQGSNITSYTDSVWWEMVTMLTVGYGDRFPVTPWGRFFGSVSMFSGVAATAIITAKIS
                     SSFLEMALRDRRGLVDSDLLKNHYIICGWKNEMNGLLTHILDSNPGLSPAKIILVNNV
                     PDAEIEALHEFPKLKNIKIIRGDFFSAEVLKRAAPEKAAKILILADATPNANGNVPTI
                     TEADARTIMTAMSLNNVAKGVPIAAEILDADMAQYLKLAHVHEIIYSREYSRLLLAMA
                     STGTGITNIFHDLLNPQGDFSIGTRDIPEEALNQNYSQLRDLLKSKYPEMTLLGVLEN
                     SGNGHRAKELALRKAQLTPNIGQLVKNLQGVKDLRFNRPIFAPKDDYIIREGAMAIVI
                     QGKGQYESIDV"
     gene            6026..6433
                     /locus_tag="VIG33_05740"
     CDS             6026..6433
                     /locus_tag="VIG33_05740"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY8270371.1"
                     /translation="MVKVTATYEGEKHCEIVHGPSQSKIATDAPKDNNGRGDLFSPTD
                     LVAAATGSCMLTVMAIMAEKDGIDIKSAYTTVEKEMLANPRRIGKLTVNIFMPKKVEE
                     SYRKKLENTAHTCPVRQSLNPEMEIPVQFHYTL"
     gene            complement(6471..6914)
                     /locus_tag="VIG33_05745"
     CDS             complement(6471..6914)
                     /locus_tag="VIG33_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEY8270372.1"
                     /translation="MASTTICSNLARTLADEYTLTLKTQNFHWNVEGPMFYSLHLMFE
                     QQYTAMQLIVDRVAERIRSYGEKAPGSYEEFKKISKIQDASPGKLSAHQMIDALVEDH
                     ETLLKDIHLFRKLADQAGDATTATLYDELALFHDKAAWMIRSQRS"
     gene            6960..7553
                     /locus_tag="VIG33_05750"
     CDS             6960..7553
                     /locus_tag="VIG33_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEY8270373.1"
                     /translation="MKNIIYTAIFTIALCAACSHKDINQPSSDNATSEHQAHQATAKP
                     VEDQGPFSAQATIKPAKAQRAKGFIHFTQSGSKVKVEAMLEGLKPGPHGFHIHEKGDC
                     STPDFTSAGSHFNPTHKAHGGLEATEKHSGDMGNIIADSKGKAKINLELSGITLGGSE
                     GIIGKAIIIHENADDFKTQPTGNSGGRIACGVIEALK"
     gene            complement(7596..8582)
                     /locus_tag="VIG33_05755"
     CDS             complement(7596..8582)
                     /locus_tag="VIG33_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8270374.1"
                     /translation="MKLGSLKSAKSLDGELVVVSRDNKKAVKVPQIASSLREAVEKWS
                     LVEKDLLKISDDLNAGRAAQAFEVNEADLHSALPRTWLFADGSAFIHHIKLVRMARKA
                     ELPATLSTVPLMYQGECGGFLAPTEDIPQVDFSHGTDFEGEVGVITDFVPMGISSEEA
                     LKRIRLFILINDVSLRGLIPEELAQGFGFFQSKPASALSPFAVTADELGEAWKDGRIH
                     LPLEVNFNGTFFGKANAKDMFFHFGQLISHAAKTRNLAAGSIIGSGTVSNEDMSMGSS
                     CLAEKRTLEQIHEGKITTPFMKEGDTIEMKMRNTKGENIFGTIFQKVRSVKK"
     gene            complement(8592..9140)
                     /locus_tag="VIG33_05760"
     CDS             complement(8592..9140)
                     /locus_tag="VIG33_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY8270375.1"
                     /translation="MFQKLATITIAFVTLISFHASAQFMGVELGVRQQSASPVATGIS
                     TNTEMAYQFGLVGAFPMVDNFLFRSGFLYTQRPVTAKIGTATTKYTFNYFDIPLTVLW
                     KLNDFGGVYGGVNLSLVASADCDNCGTTVDKKGAMPLVVGGSFKFAPNVGVDVYFEAM
                     DKINDDFKEGRAVGANLLITFD"
     gene            complement(9200..9958)
                     /locus_tag="VIG33_05765"
     CDS             complement(9200..9958)
                     /locus_tag="VIG33_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270376.1"
                     /translation="MNALNRSSQLFARLMVFNGSILFSMSLQRLVLFLVVIRHWVFGV
                     DGLTIGTSFINGVRFDLCVLGFLNIPVLFFTWAVSTDFIANTQNKVLQFLQQWMLWIY
                     LTMTTLIIHVLGLLDLMFFAANGHRWTYYDWQESGLSFFGKMWSAWGVFFTFGVIFLF
                     LILWTSRSLFTLYKLKLQGELLARLPLMKGRLSTSKSALIAVGFLLPLFVVALAARGT
                     WTPHHINKEHAEVSQIQALNQMTLSPIWAFDKKF"
     gene            10004..10663
                     /gene="maiA"
                     /locus_tag="VIG33_05770"
     CDS             10004..10663
                     /gene="maiA"
                     /locus_tag="VIG33_05770"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEY8270377.1"
                     /translation="MVEFILHNYFRSSTSYRTRIVLNYKNLPFEYKAVHLLKGEQLSP
                     EYKKLNPIGGVPTLVHNGKVIPDSYAIIEYLEEIVPTPALLPKDIYLRARVRQVCEII
                     NSSMHPLGNLRVLKYLEQKNGYNQNQKDEWAQHWGSLGLEALETNLQEFAGTYCFGDS
                     VTMADVFLVPQIVTCQRFKIDTEKYKLLTRIFNHCQKLEAFTKAHPFRQPDTPEENRI
                     K"
     gene            complement(10703..11581)
                     /locus_tag="VIG33_05775"
     CDS             complement(10703..11581)
                     /locus_tag="VIG33_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HEY8270378.1"
                     /translation="MFKIYADLTKFGIVIFVLLSGVAGYATAFMIENPFDGYHFLKTI
                     LGLYFLSSGSLAMNQVQERKLDQKMPRTANRPVASGKIKPAAAGILAFGMLMVGGNFL
                     FEVSPTAGWLGVFTILFYNGLYTLYLKPNWVYAAVPGAIPGALPITIGYAANSPDIFN
                     SESVYLFLILFLWQMPHFWTLAIKFKDDYAAGGVPVLPAAMGIEKTLYQIGLYTILYV
                     GVAIASPWFVHASWIFIFAVLPFAFKVMQEFIRFYKSKGTERWFAFFMWTNVSMLVFV
                     FVPVIDKWNFLFIGSN"
     gene            complement(11588..11914)
                     /locus_tag="VIG33_05780"
     CDS             complement(11588..11914)
                     /locus_tag="VIG33_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEY8270379.1"
                     /translation="MAGNHGDSQGHHITPLNVYLKVAGALFALTFLTVIAHHFSAQLG
                     AMAAPVAFFIATIKAVLVMLWFMHLKYDTIMNRVIFGAGFFFLALLLAFSGLDIWTRV
                     AEFSTL"
     gene            complement(11926..12591)
                     /locus_tag="VIG33_05785"
     CDS             complement(11926..12591)
                     /locus_tag="VIG33_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HEY8270380.1"
                     /translation="MSTDSHSVKGHEHHFAHHFRSAEHEYETTKEGIWLFMVTEILMF
                     GGLFIGYSIFHNIYPEMFAEGASYLDWKMGFLNTLVLIFSSLTMALGIFFLQKNEQKK
                     AVMSLAITILCGAIFMVVKYFEYTHKFHLGIFPGRLLDVAKVGVHHANLGMYFGFYYC
                     MTGLHGIHVLLGMGLISWVLFRTLRGDFNSNYYTAVEGVGIFWHIIDLIWIFLFPLLY
                     LVG"
     gene            complement(12578..14179)
                     /gene="ctaD"
                     /locus_tag="VIG33_05790"
     CDS             complement(12578..14179)
                     /gene="ctaD"
                     /locus_tag="VIG33_05790"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY8270381.1"
                     /translation="MATAAAHGHGSDKNYLNESSGLWSWLTTLDHKRIGIMYFISVLI
                     FFLLGGIMALLLRIELMYPGQTIMSADTYNQVMTYHGSIMVFMVIIPGIPAILGNFFL
                     PLHIGAKDVAFPRLNLLSWYCLMAGALLAFTTLITHKVDTGWTFYTPYSIKTGTSVTT
                     MVVAAFIMGVSSILTGLNFIATVHKLRAPGMTMYRIPLFVWAIYSTAILQVLATPVLG
                     ITLLLLSVERAFGVGIFDPALGGDPVLFQHFFWFYSHPAVYIMILPAMGVISELITTF
                     SRKVIFGYTAIAYSSLGIAGVSFFVWGHHMFVSGQSTIAGIVFSFLTMLVGVPTAIKM
                     FNWIATMYKGSIQMQSPMLFAMGFMFLFGIGGVTGIMLAVIPINVHFHDTYFVVAHFH
                     YVMVGGTLMAIMGGFYYWFPKMFGKMYNEMTARLSFVFIFIGFNVTFFPQFILGAMGM
                     PRRYFDYIPAYYSLNAISTVGSWLIAIGFLIALYTIINGLTKGEKAPMNPWGSKTLEW
                     TTSSPPPHSNFDVEPVITAGPYEYR"
     gene            complement(14190..15146)
                     /gene="coxB"
                     /locus_tag="VIG33_05795"
     CDS             complement(14190..15146)
                     /gene="coxB"
                     /locus_tag="VIG33_05795"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY8270382.1"
                     /translation="MMWFNLAQAQSFMPTEGTKIATQVDNLYGFLLVASLISFLILMG
                     GMTFFVFKYKRRTNKDKTGYITHNTFLEFLWSFIPLVIFLGVFAWGWLIYHEMRSMPK
                     DALEVHVFGKQWAWEFEYKSGVKAGNLLVVPVNKDVKLIMTSKDVIHSFYIPSFRIKQ
                     DVIPGRYTALWFNADKLGEYHVFCAEFCGTQHSGMLATLKVVPQAEYEAWLEDESQVG
                     SLPLAKRGEKLFAVKACASCHSVADAATKVGPTLWKKFGTKESIEGMVDVDVDENYLR
                     ESILNPNAKIVKGFPKGVMPAFQGQLNETELNALIEYVKTLK"
     gene            complement(15153..16031)
                     /locus_tag="VIG33_05800"
     CDS             complement(15153..16031)
                     /locus_tag="VIG33_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY8270383.1"
                     /translation="MMKFGCWSLQSKVQGVAFATLLLLLSVSPLHAYEPKPIDLTSNE
                     TAKGLEGIGIDEKIGTHLDLNLTFKNENGETVTLAKYFDKSHTPVIISPVYYSCPGLC
                     NFHLNGLTDALKQMDWTVGDKFKVVAISFDAKEQPDVALKKKASYMRLYGRPASEAGW
                     SFLTGDEANIKAFTSAVGFKFKWSEEAKEWAHASAAIVVTPEGKISRYLPGIMFDPKD
                     VKLALNEASSGKIGTFVDSLVLYCFQYNPHQSKYTLYAFSIMKLGGALMVLVLGLWLL
                     PVLIRSRGAGKKVARS"
     gene            complement(16037..16573)
                     /locus_tag="VIG33_05805"
     CDS             complement(16037..16573)
                     /locus_tag="VIG33_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8270384.1"
                     /translation="MSDSREEYNRGGMNAFIFSMVFVVLFFIYMVAIHPGVNLDEKVV
                     DPNAAVAGVTEPVFDIATVKEPWVKNDQVVAYGKKIFATNCAMCHGNEGKGDGPAGAA
                     LNPKPRNVVEGKWTQGNGAIAHFKVITNGIPGTSMAAFGHFKAADRWALVQFIESITQ
                     NKSTDTPEQIAEFAKTAK"
     gene            16789..17229
                     /gene="bamE"
                     /locus_tag="VIG33_05810"
     CDS             16789..17229
                     /gene="bamE"
                     /locus_tag="VIG33_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEY8270385.1"
                     /translation="MIRSISVFTILMSGLTLSACQTHMHDEYRKLHPGMEKNDVLEVM
                     GSPQRTQRLHGKDRWTYVFYDNQFRHEKEVQFFEGNAVYVGDPWEPEISAAQTDTKNE
                     TSNKALEEESLRKFEENPKAYSNYESEVRGADKVRIVPRFKPVQ"
     gene            complement(17248..17718)
                     /locus_tag="VIG33_05815"
     CDS             complement(17248..17718)
                     /locus_tag="VIG33_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270386.1"
                     /translation="MTDSQKKQMNEASELQAHVQALRAKVKSKEETINKLIKEKNHTK
                     DAEKVKEIIAEMVVEHKEMSKMVEEYEQSRSLLRYRYPEKGYAGTRSYERMEVKPLDQ
                     MENLSSIEAKLKRNLKTIHAQFGEPTEVKSSKKKKAQKKEEEAPSLTEPIFLQK"
     gene            complement(17810..18436)
                     /locus_tag="VIG33_05820"
     CDS             complement(17810..18436)
                     /locus_tag="VIG33_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270387.1"
                     /translation="MAICPICQSEIEITTQHYGTLFTCPKCNAVFFVDWEGHPETSSD
                     RAINETSDEISMDIPPEELGYPEETPQEIPHAMAQPEEDSSNYDFSQPLETLSSSQQD
                     LTEPKERDATDFSDIVEFGNADLGQAAFNYTLTISGIDSAAIRAQIEEALSDSKFNWN
                     VIQLMAQINGGVLIIRSMNAVKASILMQRVKYLPVKVSWRQNVLSGAI"
     gene            complement(18464..>20634)
                     /gene="secA"
                     /locus_tag="VIG33_05825"
     CDS             complement(18464..>20634)
                     /gene="secA"
                     /locus_tag="VIG33_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY8270388.1"
                     /translation="RQRKEMYRCDITYCTNNELGFDYLRDNMKFELEDYVQRELHYAI
                     VDECDSILIDEARTPLIISGPAEASTDKYIIINKIIPGLKRDVHFTMEEKSKTASLTE
                     EGNAKVEELLGLENLYDPRNIDILHHLNQALKAHHLYRLDVEYMIKDGEIVIVDEFTG
                     RLMPGRRWSDGLHQAIEAKEGVEVKNENQTLATITFQNYFRMYEKIAGMTGTADTEAT
                     EFKKIYNLDVSVIPTNKPILRKDQEDVVYKSEAAKFKAIAADVKERTAKGQPILVGTV
                     SIEKSEALSHALKLAGIRHDVLNAKAHEREAEIVAQAGRKGAVTIATNMAGRGTDIVL
                     GGNAEVMAKKLIEDETSPEYQEAYLKFKKQFEEERNKVVAAGGLYIIGTERHESRRID
                     NQLRGRSGRQGDPGESRFYLSLEDTLMRIFNGERIQKIMGMLNIPEDEPITAKMVTNA
                     IEGAQRKVEGHQFDIRKHLIDYDDVMNKQRTVIYRMRRQVLEGNDVERTILDFLGEVT
                     SGILDTHIPENGKKEEWNLDALNTALSQQFGIRLDLESLVNNSEVITEGVSKAVKETF
                     ERQKASMGPFFPQVQKMVLLQSIDNHWKEHLQVIDKIKEGINLRGYASKDPLIEYKKE
                     AFQTFENLNNIIQRDVIEKLMKVQLVAQQPGAEEALEGLRPEEADLSELDYQAPNDSD
                     IGIMEEATVSAPERQRMTFSNAPPEEQRMNRADRRKSKKR"
BASE COUNT         6088 a         4804 c         4145 g         5597 t
ORIGIN      
        1 cgcttagata gccaggattt taaaacaaag cgtgattgta aatattccga ttttcttttg
       61 tcgttcgtaa tgtgatgaaa atattgaagc tcctcttccg aaagaaaact gtcgggagct
      121 tcatgaaacc cttctataaa gcggtgaacc tgcagatgat atctctcttg aggatcccgc
      181 gtttcaaaga taagcatatt atttaaaatg cttctgaaag ccctgccagc actcctgata
      241 atcttcctgt aaaaaatctt tctgcatagc aaagtctgtc aacaaatagg gagttcgaca
      301 ttcaaacata aaggccagag tgttttcaag tttatgaggt tttaactccg cattcgtggc
      361 cttttcataa gtatcactat caggaccgtg ggctgaatag aaattatgaa ggctgcctcc
      421 gcccggaaca aaaccaccac tggttttcgc atcataaaca ccgtgaatga gtcccatata
      481 ttcactcatg cagtttcgat gaaagtaggg cggacggaag gtgtgctcag cgaccatcca
      541 tcgcggagga aaaatcacaa agtccacatt ggcgacaccg gctgtatcac ttggggaagt
      601 gagaaccgtg aaaatcgaag gatctggatg atcaaagctg acggtattta tagtgttaaa
      661 cttacgcaga tcatatttgt acggaacata gttcccatgc caagcaacaa catcgagagg
      721 tgaatgattc atctctgctt tccaaaggtg accggaaaac ttgccaataa gttcaaattt
      781 tccgtcacga tcttcaaatg ccgcgtgtgg aaccataaag tgacgaccgt gagctaaacc
      841 gtttgccccg atcggtccca aatccggcaa gcgaaaaggc gctccaaaat tttccccaac
      901 atagccacga caaacagccg cttggggatt cacctgaaac ttgattcccc gaggaaccac
      961 tacaatttcg ccaggttccg cctcaatgat tccatactca gttttaattt cgatggatcc
     1021 taattgcagg acaaaaagat aatcgccgtc cgcattcatc atatagcgag aacccatcgc
     1081 ttgattaaaa gcatacaggt ggatatgcaa acctctttgc tcaatagcag aacccacgcc
     1141 acaaacagtg cgaagtcctt ccagaaaatc ctgcggcgtt gttggatgtg gtaagggatt
     1201 ccaacgcatt tggttggggt tgatatgctt aggtttaaaa agggtgcgtt cagtcagctc
     1261 tttcagaggc acaaaaggcc tgtgcatcac agagggacga atccgataaa accaggaagc
     1321 caaattcgtc gatcgtggtg ccgtaaaggc ggagccattc aattgttccg gataaagtcc
     1381 tagcggacaa ttttgtggag aattttgatt caaaggcaat gcctgagggc gtgcttccga
     1441 agaaaagtga tttccaaatc ctgaaaaata ttttgtatct ctctctttat tcatgctgaa
     1501 aagataacca gacttcagat ctgaaagtca aagaagaatt taagaacaca tagtggttac
     1561 tgacacatca aattttgatg gttttcaggc aattttcaag acacagttaa agtaataact
     1621 tgtaaggagc ctacttatga aatctcttct caccaaatat tccaaccaac ttaaaaagaa
     1681 tggctgctat tcttggacta ttttcttcat tcgcttagtg gtggggcttg ccttcatgat
     1741 gcatggcgga gggaaaattc aaaactcttt caattggatg ggttcagact cttccgttcc
     1801 tggaatcttt caaggattag cagcactctc tgaatttggg ggcggcctgg cttggatttt
     1861 aggacttctc actcccttag catctttggg catttttttt acgatggctg tggcgacttt
     1921 gtcccatgca acccgagggg attcttttgt cgcgcatggg gctccgacct atgaacttgc
     1981 cttggtctat ctgaccatag cgctttttat gatgtgcaat ggtccaggaa aaatttcggc
     2041 agattacaag atattttcca gttaaaccgg gctctggcct aggagcttaa ccctttagtc
     2101 ttagttttaa tttttcgata aggaggtatg aaacttttct tggcattttt attctgtttc
     2161 ctcggattct ctaaagccta tgcggaggaa tcaaccagtt ccgtctatga atgcctcttt
     2221 caaaataact ttgtcactac ccaaagccta cgtatgaaag attattttga tattgaaaaa
     2281 ggtcaacgtg ccggcaaagt agatcttctt gagaatcttg aaactatcga aagtacgccc
     2341 actctggttt atcaaattcc atttttagat caacagatgt atgtgcaaat ttggcactcc
     2401 cccactgtac gtgtggattc tcaaatgtct ctttcgggtt caaatcaaac tttctctgcg
     2461 acctatcata agggttcttc gcaagaagag ttgctctgca ccaccctcgg taaataatct
     2521 cacttttaat tttcttgcca caagactttg gcccagttct aatttggaat caccatccgc
     2581 gcttggccca tttattaagc ggcaggatga ttggttatcg agggggatta caatgaaaac
     2641 atttttatta aatccactat tcatagtggt gctatttata acgtcatcca gttgggcagg
     2701 gtcacttgtt aacgtttcga ctttaaacaa aaccctgcaa aaacaaaaag caacctgggt
     2761 tgctaaggaa aattggctca ctcaactcag taaaagtgaa gcaaagagaa tgatgggttt
     2821 aagaaaaact ccaccattgg atgtgcaatt tgtggtgccc ctcagcatga ctcaagacac
     2881 attgcctcct tctttagact ggagaaacaa agatggtcga aactgggtct ctcctattct
     2941 gaaccaggga aactgtggaa gctgcgtggc ctttgcggct atcggcgtca tggaaactca
     3001 gttaaatatt tcctcactgc ttcccaatct aaacatccgt ctttctccac aacatctttt
     3061 ctcttgtgga gatggcgctt gtaattacgg ctggatgccg gaatcagccg caagccagtt
     3121 agtgagaacg ggcgttaccg atgaagcctg tatgccttat acttcaggag cttccggcaa
     3181 agacgtggct tgccaagcaa gttgctcaga tctttctcag agaatttaca aaatttctga
     3241 ttacaattcg ccaacagaaa gtacgatgga cgtggacgct atcaagcgcg ctttgcaaaa
     3301 gggtccattg atgacttctt tgaatgtcta cgcagacttc atgtcttatg ctggcggagt
     3361 ctataagcat gtgagtgggg attatctggg gggccatgct atttccatcg ttggctacga
     3421 tgatgtgagc caatctttta tcatccgcaa tagttggggc gacagctggg gagaagaagg
     3481 ctttggtcat atttcttacg aagatacctc aggagtcgga gaagccactt ggggctttga
     3541 tattccaaca ataggtggag ctatttctct cacaacaccg cgcgattaca cttatatgac
     3601 aggcacggtt tctttatccg cagaatccac tttcaataat acagacggta ttagctacgc
     3661 gatttatgga agcaacagcc aagtcatctg gtctggtgtt tgccaaggtc caaactgttc
     3721 tgttagcctt gatagctcga aatttcagga cggccgctat gaaatccaag ctctggcgac
     3781 aaatgctcat ggcgataatt taggtgcttc tacgaagcag ttcttttata tcgtaaacca
     3841 aactccagag cttaagctgt ctttcaccgg tttaggtcgc gtagacttaa gtaagcctct
     3901 caaaggacgt attgagtttt cgatctcggc ccaatcttca ggggttccaa tgagttcttt
     3961 ggaattccat tacaaagacc caagtggcaa agaaaccgtt cgtgtttcga atgtcgtatt
     4021 ggaccaaatg accacaggat ggagaaccaa tctcattcct aatggacgct atgaaatctg
     4081 gatgatcgga cgccttcaaa gcaatagctt tggtctttca atcgagactt cacattccat
     4141 agtcactatt cagaattaat ttattcaata gatttgctta ataggaaagc ccacttcggt
     4201 gggctttttt tgccaaatgc agatcaaaat ttagcttgag cgaatttcac tcatcaaagc
     4261 tttgtgcagc agcatcaaat cattgctagt ttgattcaga cgactcatca gaatcaaaga
     4321 aatgcgtttt aaaacaggca gagcaaattg gggttgagct agcgaaaatt gatcaaactc
     4381 tttacagctt aaaaccaagc actccacctc tgtgtcacaa gtcaccgtgg ccgagcgggc
     4441 ttctccttcg atcaagcctc cttctccgaa ggcggcgttt tgttcccctt tgagcaccgc
     4501 cactttatag cggtcccctt ctggcgtgat tttctcaatg ctaacttgtc cttttaggag
     4561 tacgaaaaat tcagatccgg cttccccttg ggtaatcaaa acgtgtccct tggaatactt
     4621 gcaaggtttc ataattctcc gaaggcttaa gagcgcttcg ggcaaatgtt taatatcctg
     4681 aaacaatgat acgttctgaa gaacattgtc atgggagcta gacatcgatg gattcatact
     4741 gtcctttacc ttgaatcaca atggccattg caccttcgcg aataatataa tcatctttcg
     4801 gagcgaaaat gggacgattg aaacgcaaat ctttgacacc ttgaaggttc tttaccaatt
     4861 gcccgatatt tggtgtgagc tgtgcttttc ttaaggccaa ctcttttgct cgatgcccat
     4921 tgccagaatt ttctaaaact cccaaaagcg tcatctctgg atatttactt ttcaagaggt
     4981 ctcgcaactg actgtagttt tgatttaacg cctcttcagg aatatctcga gtccctatgg
     5041 aaaaatcgcc ctgaggattc aaaagatcgt gaaaaatatt tgtaattccc gtacccgtcg
     5101 aagccatggc caacaatagg cgggaatact ctcggctgta tataatttca tgaacatgtg
     5161 ccagctttaa atactgagcc atatcggcat ccaaaatttc cgcagcaatg ggcacccctt
     5221 tagcgacatt gttcaaagac attgctgtca taatcgtgcg ggcatcggcc tctgtgatcg
     5281 tcggaacatt tccattcgca ttcggagtcg cgtccgcaag aataagtatc ttggccgctt
     5341 tctcgggagc cgccctcttc aggacttccg cagaaaagaa atcaccacgg ataattttta
     5401 tgttctttaa tttcggaaat tcatgaagag cttctatttc cgcatcaggg acattattaa
     5461 ctagaattat tttagcagga gaaagtcccg gatttgaatc cagaatatgg gttaagaggc
     5521 cattcatttc attcttccaa ccacagataa tataatgatt tttgagtaaa tcgctatcca
     5581 cgagtcctct cctatccctt aaagccattt ctaaaaagct agacgaaatt tttgcagtga
     5641 tgatggcagt ggcggccact ccagaaaaca tactgacgga accgaaaaaa cggccccacg
     5701 gagtcaccgg aaaacgatct ccataaccca cagtcagcat ggtgaccatt tcccaccaca
     5761 cggaatctgt ataacttgtg atattagaac cctgcacacc ctgctcgaaa agataaacga
     5821 tgaaggcgtt gcctaaccac acggcgagaa aaaaagtaga agccaccaga aacggctgcg
     5881 caaataaaaa tgctttcgat cgaagcaata ttttttttac ttttttcaca aacttcccca
     5941 aagatctcgt gatcttatga tgacggactg ttgtgattct tgtaaagacc agctagactc
     6001 agcgaaagtc gtgaaaggac tcagtatggt taaagttacc gctacttatg aaggtgaaaa
     6061 acattgtgag atagttcacg gtccctccca gtcaaaaata gccaccgatg caccgaagga
     6121 taataatggt cgaggtgatc ttttttcacc caccgatctt gttgccgccg ccacaggaag
     6181 ttgcatgcta acggttatgg caatcatggc tgaaaaagat ggtatagaca ttaaaagtgc
     6241 ctacaccact gttgaaaaag aaatgcttgc gaaccctcga cgtattggca aactcactgt
     6301 gaacatattt atgccgaaaa aggtcgaaga atcttatcgt aaaaaattag aaaatacagc
     6361 acatacctgt cctgtcagac agagcctcaa tcctgagatg gaaatccctg tgcaatttca
     6421 ttacactctc taatttcaat aataatcatc ctaaacttta ggatctttat ttaagatctc
     6481 tggctacgaa tcatccatgc ggctttatca tgaaagagtg cgagctcgtc atataaagtt
     6541 gccgtagtgg catctcccgc ttgatcggcc aatttcctaa agagatggat atcctttaga
     6601 agcgtttcat gatcttccac caaggcgtca atcatctgat gcgcgctcaa tttaccagga
     6661 gatgcatcct ggatttttga gattttctta aactcctcgt aacttccagg agctttttcc
     6721 ccataagagc gaattctttc ggccacacga tcaactatca gttgcatcgc ggtgtattgt
     6781 tgttcaaaca tcaaatgcaa cgaatagaac atcggcccct ccacattcca gtggaagttt
     6841 tgtgtcttca acgtcagcgt gtactcatcc gccaacgtcc ttgctaaatt actacaaatt
     6901 gtcgtgcttg ccataaatcc tcccttgttc ttaatgagcg gctgtattag cgtttactta
     6961 tgaaaaacat tatctataca gccatcttta ctatcgccct ttgtgcggct tgctcccaca
     7021 aagacatcaa ccaaccctct tcagacaatg ctacaagtga gcatcaggct caccaggcaa
     7081 ctgcaaagcc tgtcgaagat caaggtccgt tttccgctca ggcaacgatc aagcctgcca
     7141 aagcacaaag ggcaaaaggt tttattcatt tcacgcaatc tggaagcaaa gttaaagttg
     7201 aagcaatgct cgaaggctta aagccaggac ctcatggatt tcacatccac gaaaaaggtg
     7261 actgtagtac gcctgatttc acaagcgctg gtagccattt caatccaact cataaagccc
     7321 atggcggcct cgaagctact gaaaaacaca gcggagacat gggaaatatt attgccgact
     7381 caaaaggcaa agcaaaaatt aatcttgaac tcagtggtat tactctcggc ggttctgaag
     7441 gaatcatagg taaagccatt attattcatg agaatgcgga tgactttaaa actcagccaa
     7501 caggaaattc gggtgggcga attgcctgcg gcgtgattga agctttaaaa taataatctg
     7561 gtcgtttttc aattacctag agttcgctct aaagatcact ttttgacaga gcgaactttt
     7621 tgaaaaattg ttccaaaaat attttcgccc ttcgtattcc tcatcttcat ttcgatcgta
     7681 tcgccctctt tcataaaggg cgtcgtgatt tttccctcat gaatttgttc aagagttctc
     7741 ttctctgcca agcagcttga gcccatggac atatcctcat tagaaacggt tccgcttcca
     7801 atgatagacc ctgccgccaa attacgagtt tttgccgcat gcgaaatcaa ctgaccgaaa
     7861 tgaaaaaaca tatcctttgc attggctttg ccgaagaagg ttccgttaaa attcacttcc
     7921 agtggtaaat gaatgcggcc gtctttccaa gcttcgccga gctcatccgc tgtcactgca
     7981 aatggagaca acgcagaagc gggcttgctt tgaaagaagc caaatccctg agccagttct
     8041 tctggaatga gtccacgcaa ggaaacgtca ttaatcaaaa taaacaagcg gatcctcttt
     8101 agagcctctt cagaacttat gcccatcggt acaaagtctg tgatgacgcc tacttctccc
     8161 tcgaagtcag tcccatggga aaaatccacc tgtggaatat cctcggtggg ggccaagaat
     8221 ccaccgcact ctccttgata cattaaagga accgtactta aggttgcagg cagctcggct
     8281 ttgcgagcca tgcggacaag tttaatatga tgaataaagg ctgagccatc cgcaaaaagc
     8341 caagtgcgcg gaagagccga atgcaggtcc gcttcgttga cctcaaaggc ttgagcggct
     8401 cttcctgcat ttaagtcgtc agaaattttc aaaagatctt tttcaaccaa agaccatttt
     8461 tccacggcct cacgaagtga agacgcgatc tgaggaactt taacagcttt tttattatcg
     8521 cggctgacaa cgaccagttc cccgtcaagg gacttggcag atttaagaga acccagtttc
     8581 atattttatc cttagtcgaa agtaataagc aggttcgccc caacggcgcg cccctctttg
     8641 aagtcatcat ttattttatc catcgcttca aaatagacat cgacaccgac atttggagcg
     8701 aatttaaaac ttcctccaac taccaaaggc atcgctcctt ttttatcaac agtggtaccg
     8761 caattatcac agtctgcaga ggcgactaaa gataaattca caccgccgta aactccacca
     8821 aaatcattaa gcttccaaag aaccgtcaat ggaatatcaa aataattaaa agtgtacttc
     8881 gtcgttgccg tgccaatttt cgcagttaca ggtctttgcg tatagaggaa gcctgaacgg
     8941 aacaagaaat tatcgaccat cggaaaggcg ccaaccaaac caaattgata agccatctct
     9001 gtgtttgttg aaatacctgt cgccaccggt gaggccgatt gttgtcggac acccagttcc
     9061 acgcccataa actgtgctga agcatggaaa gaaataagcg tcacaaatgc tatggttatt
     9121 gttgcaagct tctgaaacat aaatacctct tctgccctct cacgggactt gggcatattc
     9181 ttccatagaa tagttcgatt taaaactttt tatcaaaggc ccaaatagga cttaatgtca
     9241 tttgattcaa agcttgaatt tgactcactt ccgcatgctc cttatttata tgatgcggag
     9301 tccaagtccc tcgcgcagct aatgcgacaa caaacagagg caataaaaaa cctactgcta
     9361 ttaaagcact ttttgatgta cttagtctcc ccttcataag aggaagtcgt gccaggagct
     9421 ccccttgaag cttcaatttg tacaaagtaa aaaggctgcg ggaagtccaa agtattaaaa
     9481 atagaaaaat aactccgaag gtaaaaaaaa cgccccaggc actccacatc ttcccaaaga
     9541 atgacaatcc actttcttgc caatcataat aagtccagcg gtgcccgttt gccgcaaaaa
     9601 acatgagatc gagaagaccc aatacatgaa taattagagt cgtcatcgtc agataaatcc
     9661 acagcatcca ttgttgcaaa aactgaagga ccttgttctg tgtattcgcg ataaaatctg
     9721 tactgacagc ccaggtaaaa aatagcactg ggatattaag aaatcctaaa acacacagat
     9781 caaagcgaac tccattaata aaacttgtgc caatggttaa gccatcgaca ccgaaaaccc
     9841 aatggcgaat caccacaaga aacaatacca aacgctgcag gctcatagag aagaggatcg
     9901 agccattgaa aaccattaac ctggcaaaga gctggcttga tctattcaat gcattcatac
     9961 atccaccttt gactctccca aaacttagta gtgatattag attatggtag aatttatttt
    10021 acacaactat tttcgtagct cgacttctta ccgcacccgt atagtactga actataaaaa
    10081 tcttcctttt gaatataaag ccgtgcattt acttaaaggt gagcagctta gccccgaata
    10141 caaaaaactc aatcccatcg gcggggttcc aactctggtt cataacggca aagtcatacc
    10201 tgattcttat gctattattg aatatctaga agaaatagtc cctactccag cgctgttgcc
    10261 aaaggatatt tatctgagag cccgagttcg ccaagtttgt gaaattataa attcctcaat
    10321 gcatcccctg ggcaaccttc gtgtcttaaa atatttagaa caaaaaaatg gttataatca
    10381 aaatcaaaaa gatgagtggg ctcagcattg gggatcctta ggcctcgaag ccttggaaac
    10441 taacttgcag gaattcgcgg gcacttattg tttcggtgat tccgtcacca tggcggatgt
    10501 atttctcgtt ccacaaatcg taacctgcca acgcttcaag attgatacag aaaaatacaa
    10561 actgctcacc aggattttca accattgcca aaaactcgaa gcctttacca aggctcatcc
    10621 cttccggcaa ccggacactc ccgaagaaaa cagaatcaaa taacattagt cacgccgcat
    10681 ccgaaaaagg tacggcgtac cctcagttgg agccgatgaa gaggaagttc catttgtcga
    10741 tgacgggaac gaaaacgaaa acgagcatgc tcacgtttgt ccacatgaag aaggcgaacc
    10801 atcgttctgt tcccttggat ttgtaaaagc ggatgaattc ttgcattact ttgaaggcaa
    10861 agggcagaac cgcaaaaatg aagatccaac tagcatggac aaaccacgga gatgcaatag
    10921 ccacgcctac atataaaatg gtgtaaaggc cgatttgata aagagttttt tcaattccca
    10981 ttgctgctgg caatacgggg actccacctg ctgcataatc atccttgaac ttgatggcca
    11041 aggtccaaaa atgtggcatc tgccaaagaa acaaaatcag gaataaatag acagattctg
    11101 aattgaaaat gtcgggggaa ttggcagcat acccgatcgt aatcggaagt gcgcccggaa
    11161 ttgcaccagg aactgcagca taaacccaat tcggctttaa atagagagta taaagtccat
    11221 tataaaataa aatcgtgaaa acccccaacc atcccgcagt tggagaaacc tcgaaaagaa
    11281 agttcccgcc caccatcaac atcccaaaag ctaagattcc cgcagcagcc ggtttgattt
    11341 taccactagc cacagggcga tttgcggttc gaggcatttt ctggtccagc tttcgctctt
    11401 ggacttggtt catcgccaag cttccagaac ttaaaaaata aagaccgaga attgttttta
    11461 ggaagtgata cccgtcaaaa ggattttcaa tcatgaaagc cgtggcatag ccagccactc
    11521 cagaaaggag aacaaagatg actataccga acttagtgag atccgcgtaa attttaaaca
    11581 cgacaaatta caaagtactg aattctgcaa cacgtgtcca aatatctaac ccgctaaatg
    11641 ctaataaaag tgctaagaaa aagaatcctg caccaaagat cacgcgattc attatggtgt
    11701 catatttcaa atgcatgaac cacaacataa ccaagacagc tttgattgta gcaataaaga
    11761 aagctaccgg agccgccatt gcacccagct gtgcgctgaa gtgatgagcg atgactgtca
    11821 aaaaggtgag ggcaaataaa gcccccgcca cttttaagta tacattgagt ggagtgatgt
    11881 gatgtccctg actatcgccg tgattgcctg ccattgtatc ctcctctagc cgaccaaata
    11941 aagtaatgga aacaagaaaa tccaaattaa atcgataata tgccagaaaa tgccgacacc
    12001 ttcgaccgct gtgtaataat tcgaattgaa gtcaccgcga agagttcgga aaagaaccca
    12061 agaaataagc cccataccca aaagaacgtg gataccatgc aagcctgtca tgcagtagta
    12121 gaaaccaaaa tacattccta aatttgcgtg atgaactccc acttttgcga catctaaaag
    12181 tctgcccgga aaaataccca agtgaaactt atgtgtatac tcgaagtact ttaccaccat
    12241 gaaaattgcg ccacataaaa tagtgatcgc caaagacatg actgctttct tttgttcatt
    12301 tttctgcaag aaaaagatcc ccaaagccat cgttaatgaa gagaagatca aaacgagggt
    12361 gtttaagaaa cccattttcc aatcaagata actcgcgcct tcagcaaaca tctctggata
    12421 gatattatga aagatggagt atccgatgaa aaggccaccg aacatgagga tttcggttac
    12481 cataaacagc caaatgcctt ctttagttgt ttcatactca tgctcagcac ttctaaagtg
    12541 atgagcaaag tggtgttcgt gtccctttac agaatgacta tctgtactca tatggccccg
    12601 cagtgataac tggttctaca tcaaagttag aatgtggagg aggagaagat gttgtccact
    12661 cgagagtttt agatccccat ggattcatcg gtgctttctc acccttcgtt aaaccattaa
    12721 taattgtata caatgcaatc aagaaaccga tagcgatcaa ccaagatccc acggtcgaaa
    12781 ttgcattcaa agagtaatag gctggaatat aatcaaaata acgacgtggc atacccatcg
    12841 cacccaagat aaattgaggg aagaaggtca cgttaaagcc gatgaagata aagacaaacg
    12901 acaaacgcgc ggtcatttcg ttatacattt ttccgaacat ttttggaaac cagtagtaga
    12961 atccacccat gatcgccatc aatgttccac ccaccatcac ataatggaaa tgggcgacca
    13021 caaagtaagt atcatggaaa tgcacattta taggaataac ggccaacata atgccggtga
    13081 ccccaccgat gccgaacaag aacatgaagc ccatcgcgaa caacatcgga gattgcattt
    13141 ggatggaacc tttgtacatg gtcgcaatcc agttgaacat tttaatcgcc gtaggaacgc
    13201 ccaccagcat ggttaagaac gagaacacaa ttcccgcaat tgtggattgt ccagagacaa
    13261 acatatgatg tccccaaaca aagaaggaaa ctcctgcgat acccaatgaa gaataggcaa
    13321 tcgctgtgta accgaagatc actttacgag agaaggttgt gataagctca ctgatcaccc
    13381 ccatcgcagg caagatcatg atataaaccg caggatgaga atagaaccag aagaaatgct
    13441 ggaacaacac cggatctcca cccaaagccg gatcaaagat ccccactcca aaagctcttt
    13501 caacagaaag aagcaaaaga gtgatcccca ataccggagt cgccaacacc tgcaagatcg
    13561 ctgtcgaata aatcgcccat acaaacaatg gaatgcgata catagtcatg ccaggagcac
    13621 gcaacttatg aacagtcgca atgaagttca aacctgtcag gatggaagaa acccccatga
    13681 tgaaggcggc cacaaccatg gtcgtaactg aagttcccgt tttaatggaa taaggagtat
    13741 agaaagtcca acctgtatcg actttatgag taattaaagt cgtaaaggca agaagagctc
    13801 cggccattaa acagtaccaa gaaagtaagt tgagccttgg aaaagccaca tctttcgctc
    13861 caatatgcag aggcaaaaag aagtttccta agatggctgg aatccccggg ataatcacca
    13921 tgaaaaccat aatgcttcca tgataggtca tcacttgatt gtaagtgtct gctgacatga
    13981 ttgtctgacc tgggtacatg agctctatgc gaagcaaaag agccatgatt ccacccaaaa
    14041 ggaagaaaat caggacagag ataaagtaca tgatgccaat acgtttatga tccagagtcg
    14101 ttaaccaaga ccatagaccg ctagattcgt ttaaataatt cttatcacta ccgtgaccgt
    14161 gggctgcagc tgttgccatg aggccctcct tatttcaatg tcttaacgta ctcgataagt
    14221 gcgttaagtt ccgtttcgtt caattgacct tggaaagcag gcataacccc ttttgggaag
    14281 ccttttacaa tcttagcatt tggattcaag atggactcac gaagataatt ttcatcgacg
    14341 tcaacatcaa ccatgccttc gatactttct ttagtaccga attttttcca aagtgttggg
    14401 cccacttttg tcgctgcatc agcaacagag tggcaagagg cgcaggcttt cactgcaaat
    14461 aacttttccc cccgttttgc caaaggcaaa cttcccacct gggattcatc ctccaaccag
    14521 gcctcatatt ctgcttgggg aacaaccttg agcgtcgcta acataccgga atgctgggta
    14581 ccgcagaact ctgcgcagaa tacgtggtac tcccccaatt tatcagcatt gaaccacaaa
    14641 gcagtataac ggccaggaat cacgtcttgt ttaatacgga aactcggaat gtaaaagcta
    14701 tgaataacat ctttcgaggt catgatgagc ttcacatctt tattcactgg aactacgagt
    14761 agattgccag ctttaacacc cgatttatat tcaaattccc aagcccattg tttaccaaac
    14821 acatgtactt ctaaagcatc tttaggcatg cttctcattt catgataaat gagccatccc
    14881 caagcaaata ctcccaagaa gatcacgagt ggaataaaag accacaagaa ctccaagaaa
    14941 gtgttatgag taatatagcc ggtcttatct ttatttgttc ttcgtttata tttgaaaaca
    15001 aagaaagtca ttccgcccat aaggataagg aatgaaataa gactcgccac cagaaggaat
    15061 ccgtagagat tatcgacttg agtcgcaatc ttcgtacctt cggtcggcat aaaggattgt
    15121 gcctgagcta aattaaacca catcattgac cctcaactcc tcgccacttt tttccccgct
    15181 ccacgtgagc gaattaaaac tggcagtaac cataagccca acaccagaac cattaaggct
    15241 ccgcccagct tcataatact aaaagcatac agagtatatt tgctctggtg gggattgtat
    15301 tgaaaacaat aaagaaccaa tgaatcaaca aaggttccga ttttgccgct tgaagcctca
    15361 ttcaatgcaa gcttcacgtc tttagggtca aacataatcc ctggcaaata acgagaaatt
    15421 tttccctctg gagtcaccac aattgccgcg gatgcatggg cccactcttt ggcctcttca
    15481 ctccatttga acttaaaacc aaccgccgaa gtgaaggcct taatattagc ctcgtctcca
    15541 gtgaggaaac tccaaccggc ttctgaggcg gggcgaccat aaagcctcat ataagaggct
    15601 tttttcttca aagctacatc tggctgctct ttagcatcaa aactaatggc caccactttg
    15661 aatttatcgc cgacagtcca atccatttgc tttaaagcat ctgtcagacc atttaaatga
    15721 aaattgcaaa gacctgggca agaataataa accggactaa taataacagg cgtatggctt
    15781 ttatcaaaat acttagccaa ggtcacggtt tcgccatttt catttttaaa agtaagattt
    15841 aaatctaaat gagtcccgat cttctcatca attcctatac cctcaagtcc tttagcggtt
    15901 tcgttggagg tcaggtctat aggttttggc tcataagcgt ggagcggaga taccgacaaa
    15961 agcagcaaaa gagtcgcaaa tgcgactcct tgaactttcg attgcaagct ccaacaacca
    16021 aacttcatca tgcttcctac ttcgcagttt tagcgaattc tgcaatttgc tcaggggtat
    16081 ccgtagactt attctgagtg attgattcga tgaattgaac aagagcccaa cgatccgccg
    16141 ctttaaagtg gccaaatgcc gccattgaag tgcctggaat gccgttggta ataactttaa
    16201 aatgagcaat tgccccgtta ccttgggtcc attttccttc aaccacattg cgtggttttg
    16261 gattcaaagc tgcacctgcg ggtccatccc ctttaccttc gttaccgtgg cacattgcac
    16321 agttcgtagc gaatattttt ttaccatagg ctacgacctg atcattttta acccacggct
    16381 cctttacggt cgcaatatca aaaacaggct cggtgacacc agctacagct gcgttgggat
    16441 ccacgacttt ttcatctaga tttacacctg gatgaattgc aaccatataa ataaagaaca
    16501 ggacaacaaa caccatggaa aaaataaagg cattcattcc accgcgatta tattcttctc
    16561 tagaatcaga catagcaccg cactccccaa agggacgttt tatgatcaat tggttttcac
    16621 gttaatgtgt attgattagt agcgtaaaac gaaagtgctc gcaagaattt taccaaacct
    16681 tcctatgcct atcgcattgt ataaaaacga aaatatgcct caaaaagaga ctgccatttc
    16741 cttttttcca aaaagaagcg aaaataaagg gaaggggtga ggtgccgtat gattcgctct
    16801 atttcagtat tcaccatact tatgagtggg cttacacttt cagcgtgtca gacccatatg
    16861 catgatgagt accgcaaact tcatccaggc atggaaaaaa atgatgtcct tgaggtgatg
    16921 ggcagccctc aacgaaccca gcgacttcac ggcaaggacc gatggaccta tgtgttctac
    16981 gataatcaat tccgccatga aaaagaagtg cagttctttg aagggaacgc tgtttatgtc
    17041 ggtgaccctt gggaacccga aataagtgcg gcgcaaacag acacaaaaaa cgaaacttca
    17101 aataaagcct tggaagaaga aagcttacgg aaattcgaag aaaaccctaa agcctattct
    17161 aattatgaaa gcgaagtccg tggcgccgat aaagttcgca ttgtaccccg ctttaaaccg
    17221 gttcaataaa caaaatttta tggttcatta tttttgcaaa aaaatcggct ctgttagtga
    17281 tggcgcttcc tcttcctttt tttgagcttt tttcttttta gaagacttaa cttcagttgg
    17341 ttcaccgaat tgcgcatgaa tggttttcaa attgcgcttt aacttggctt caatactcga
    17401 tagattttcc atctgatcca gaggctttac ctccatgcgt tcatagctgc gggtgccggc
    17461 atagcctttt tcagggtaac gatagcgaag aagactgcgg ctctgctcgt attcttctac
    17521 cattttactc atttctttat gctccaccac catctcggcg ataatttctt tcaccttttc
    17581 agcatctttg gtgtggttct tttctttaat cagcttatta attgtttctt cttttgattt
    17641 tactttggcc cgaagtgctt gaacgtgagc ctgaagttct gaggcttcat tcatttgctt
    17701 tttctgggaa tccgtcatat ttccctcctc ttttttctcc tctttccctt cattcgcaaa
    17761 tgtgctagtg ccctggatta aacttagaac taagagaaat ataagaattt taaatggcgc
    17821 ctgagagaac attttgcctc caactgacct tcaccgggag atatttcact ctttgcatca
    17881 gtatactggc tttgacggca ttcatagatc gaattattaa aaccccgcca tttatctggg
    17941 ccatgagctg gatgacattc caattaaatt tagaatcgga aagagcttct tcgatctgcg
    18001 ctcgaatggc agctgaatca atacctgaaa tggtgagggt ataattaaaa gccgcttgtc
    18061 ctaaatctgc gttaccgaac tctactatat cagagaaatc cgtagcgtct ctttccttag
    18121 gttctgtaag gtcttgctga gaactcgaaa gcgtttctaa aggttgggaa aaatcataat
    18181 ttgaagagtc ctcttcaggc tgcgccattg cgtgtgggat ttcttgtgga gtttcttcgg
    18241 gataaccaag ttcctcgggg gggatatcca ttgaaatttc gtcggaggtt tcgttgatcg
    18301 cacgatcgct ggaggtctcg ggatgcccct cccaatctac aaagaaaacc gcgttgcact
    18361 tgggacacgt aaacaaagtc ccatagtgtt gcgtggtgat ttctatctct gactgacaaa
    18421 tggggcaaat agccaaggca atggttccct ttgcttcatt agattatctc ttcttactct
    18481 tacggcgatc cgcacgattc attctctgct cctccggagg agcattgctg aaagtcatcc
    18541 tctgtctttc aggagccgac accgttgcct cttccattat tccaatgtct gaatcattcg
    18601 gcgcttggta atccaactcg ctcaagtctg cctcttcagg tcttaaaccc tcaagggcct
    18661 cttcggcacc aggctgttgg gccaccaatt gaaccttcat caacttttca ataacgtctc
    18721 tttggatgat attattaaga ttttcaaaag tttggaaagc ttccttctta tactcgatca
    18781 aaggatcttt ggaagcatat ccgcgtaaat taataccctc tttaatttta tcaataactt
    18841 gtaaatgctc tttccaatgg ttgtcgatgg attgcaagag aaccattttt tgaacttgcg
    18901 ggaaaaaagg ccccatggag gctttttgac gctcgaaagt ctctttcaca gctttactca
    18961 caccttcggt gatgacttca gagttattga ccaaagattc caaatccaaa cgaatgccaa
    19021 actgttgaga taaagcggtg ttcagtgcgt ctaagttcca ttcttccttt ttgccattct
    19081 caggaatgtg agtatctaag atacccgagg tcacttcacc caagaaatcc agaattgttc
    19141 tttcaacatc attgccctct agaacctggc gacgcatgcg atagataact gttctttgct
    19201 tattcatgac atcatcataa tcaatcagat gcttacgaat atcgaactga tggccctcaa
    19261 ctttccgttg agctccttca atggcattag tgaccatttt agcagtgatt ggctcgtcct
    19321 caggaatatt taacattccc atgatttttt gaatacgctc cccattaaag atgcgcatca
    19381 aagtatcttc taaggagaga tagaatcgag attcaccggg gtccccttga cggcccgaac
    19441 gaccacgaag ctgattatca atacgacgag actcatgacg ttcggttccg atgatgtata
    19501 aaccaccggc ggccacgact ttatttcgct cttcctcaaa ttgttttttg aattttagat
    19561 atgcctcttg gtattcaggc gaggtttcat cttcaatcaa ctttttagcc atgacttcag
    19621 cattgccacc caagacgata tccgttccgc ggcctgccat attggtggca atagtcacag
    19681 ctcctttacg tcctgcctgg gccacaatct cagcttcgcg ctcatgcgcc ttcgcgttca
    19741 aaacatcatg acgaattccc gcaagcttca gggcgtggct cagagcttca gatttctcga
    19801 tgctcacagt tcctaccaga atcggctgcc ctttcgctgt acgctctttc acatccgcag
    19861 cgattgcctt aaattttgcg gcctcagact tataaaccac gtcttcctga tctttacgca
    19921 agatcggctt attcgttgga ataaccgaaa catcgagatt atagattttt ttaaactcgg
    19981 tggcctcagt gtcggcggtt cccgtcatgc cggcaatttt ttcatacata cggaaatagt
    20041 tttggaaagt gatggtcgcg agagtttgat tctcgttctt cacttcaacg ccttctttgg
    20101 cttcgatcgc ttgatgaaga ccgtcactcc agcgacgacc cggcatcaaa cgtccggtga
    20161 attcatccac gataacgatc tccccatcct taatcatgta ctcaacatcc aaacgataaa
    20221 gatgatgagc tttcaaagct tggttcagat gatgaagaat atcaatattg cggggatcat
    20281 aaaggttctc taaacccaac aattcttcaa ctttcgcgtt tccttcctcc gtcaaagaag
    20341 ctgttttaga tttttcttcc atggtgaaat gcacatcgcg cttcaaccca ggaatgattt
    20401 tattaataat aatgtattta tccgtggaag cttccgcagg accggagatg ataagcggtg
    20461 tccgggcttc atcgatcaaa atggaatcgc actcatccac aatcgcataa tgaagctcac
    20521 gttgcacata atcttcgagt tcaaatttca tattatctcg aagataatca aaacccagct
    20581 cgttgtttgt gcaataagtg atatcacaac ggtacatttc tttacgttgt cgat
//
LOCUS       DATBTE010000153         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1249078, whole genome shotgun sequence.
ACCESSION   DATBTE010000153 DATBTE010000000
VERSION     DATBTE010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249078"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VIG33_05830"
     CDS             complement(<1..562)
                     /locus_tag="VIG33_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270389.1"
                     /translation="MTKTVFGLILLFWMALHTSQSAIDLADFLLIIAAFKTAIKTHEI
                     LSLLKSFRPYWLWPLWLTVIILGFVMGAPVEAKVALKLVWEFRWFLSFMCIIYLFKKM
                     NWDEVQIRSFSLVLLAFSILDVVLFFVDYDKDPRAGGLFGHSMPFAHTMGPSALLLLF
                     IGIKKSLEKSQSLLWRGVYFLTPLLAS"
     gene            676..1413
                     /locus_tag="VIG33_05835"
     CDS             676..1413
                     /locus_tag="VIG33_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8270390.1"
                     /translation="MQNPEYKEQVNNKTISVVICAYNEERTIGAVLEKLHRLPFITEI
                     ICVNNGSTDSTGSIIKKQSDIDTRIKLVEVLKNQGLGHGLCQGISQTKCDIVVRQDAD
                     LEYDPNELINLIDPIANGHGQVCFGSRMLVRKAHRAQYFYNYLANKFFTFIADLLANL
                     NLTDVETAAKAFDGPLIRSIPFYTKGFQIEIEMLFRLRAHGAIFYEVPISYYGRSIAE
                     GKKIRLKDAFTTTWALLTYGVFWKLLK"
     gene            1410..2300
                     /locus_tag="VIG33_05840"
     CDS             1410..2300
                     /locus_tag="VIG33_05840"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8270391.1"
                     /translation="MKKKILMTGATGYLGSNLLKNLVPDYDVVILKRQKSNTIRINSI
                     LDKVTSVNMEETNFPELFKDQNFSGVIHCATNYGRDENDLDDTIEANLILPLHLLKNA
                     FENKVEFFINTDTILDKRINYYSLSKSQFKDWFLFFSNKIKSINIELEHFYGKGDDPS
                     KFVSKIIKDLVLNEKKDLELTLGEQKRSFIYIDDVVNAFRTILENIAQLPAEYSSFQV
                     GSKKQISIRDFVILTKELCHNTQTHLKFGALPYRKNEAMSVSLDISKLESLGWSEKSS
                     LHEGLRETIAFEKSALRSQE"
BASE COUNT          785 a          562 c          489 g          679 t
ORIGIN      
        1 cactggccaa taagggggtc aaaaaataaa cccctcgcca tagcagactc tgactttttt
       61 ctaatgattt tttaattcct ataaaaagaa gtaataacgc cgaaggtccc atggtgtggg
      121 caaaaggcat cgagtggcca aaaagccctc cagctcgggg atctttatcg tagtcgacga
      181 aaaacaaaac cacatccaaa atagaaaaag ccaataacac caaagaaaaa gatctgattt
      241 gcacttcatc ccaattcatt tttttgaaaa gataaatgat gcacatgaag ctgaggaacc
      301 atctgaattc ccaaaccaat ttcagggcca ccttggcttc aacgggtgcc cccataacaa
      361 agccgaggat aatgaccgtt agccacaggg gccaaagcca ataaggtcga aaggatttca
      421 aaagactgag aatttcatga gttttgatgg ctgtcttgaa tgccgcaatg atgagaagaa
      481 aatcggccag atcaatggcg gattgagaag tatgaagggc catccaaaat aacaatatca
      541 agccaaatac agtctttgtc atttcaacag tcttatggag ggctcgctct caagacaagg
      601 gcctttactt gtttatgcta cgcgctaacg aaagaattga caaggatcga gatcattaca
      661 taaatgaagg aacctatgca aaatcctgaa tataaagagc aagtgaacaa taaaaccatc
      721 tctgtcgtta tctgcgctta caatgaagag agaaccatcg gcgccgtttt agaaaaactg
      781 caccgccttc ccttcatcac tgaaattatc tgcgtaaaca acggctccac cgactccacc
      841 ggctccatca tcaagaaaca gtctgatatc gacacgcgca tcaagctggt tgaagtcctt
      901 aaaaatcaag gactgggtca tggtctctgc caagggattt cccaaacaaa atgtgatatc
      961 gtcgttcgtc aagatgctga tttagaatac gaccccaatg agctgattaa tctcatcgac
     1021 cctattgcca acggccacgg ccaagtttgc tttggctctc gcatgttggt cagaaaagct
     1081 cacagagccc agtattttta taactaccta gctaataagt tcttcacctt cattgcggac
     1141 cttctggcaa acctgaacct tacagacgtg gaaacggctg caaaagcttt cgacgggccg
     1201 ctgattcgca gcattccttt ttatacaaaa ggctttcaaa tagagattga aatgctcttt
     1261 agactgcgcg cccacggtgc tatcttttat gaagttccca tttcttacta tggtcgctcc
     1321 attgcggaag gcaagaagat ccggctcaaa gatgctttca ccacaacctg ggctcttctg
     1381 acttatgggg tcttctggaa acttctaaaa tgaaaaaaaa aattctgatg actggcgcta
     1441 ccgggtatct cggcagcaac ctcctcaaga atttggttcc tgattacgat gtagtcatcc
     1501 tcaaaagaca aaaatccaat acgatccgaa tcaattctat tctcgacaaa gtcacctccg
     1561 ttaacatgga ggagacaaat tttccggaac tttttaagga tcagaatttc tcaggagtca
     1621 ttcattgcgc gactaattat ggtcgggatg aaaacgatct cgatgacacc attgaagcta
     1681 atctgattct ccctcttcat cttttgaaaa atgctttcga aaataaggtc gagttcttta
     1741 taaataccga caccatttta gataaacgaa tcaattatta ttctttatcc aagtcccagt
     1801 tcaaagattg gtttttgttt ttttcaaaca aaatcaaatc cattaatatt gagttggaac
     1861 acttttatgg aaaaggggat gatccttcca aatttgtctc taaaatcatt aaagacctgg
     1921 tgcttaacga aaaaaaagat ctggagctga ctctaggtga gcaaaagaga agttttattt
     1981 atatcgatga tgtggtgaat gcctttcgca cgattttaga aaatatcgct cagcttccag
     2041 cggagtatag ctcctttcag gtcggctcca aaaaacagat ttccattcgg gacttcgtca
     2101 ttctaaccaa agaattgtgt cataatactc agactcatct gaagtttggt gctcttcctt
     2161 atcgaaagaa tgaagccatg tccgtcagcc ttgacatttc taaattggag agccttggat
     2221 ggtctgaaaa aagctctctc cacgaaggac ttcgagagac cattgcattt gaaaaaagcg
     2281 ctttgcgatc tcaggaataa ttaaacgagc cagcagttga tttcttgcga ctcaagcact
     2341 aggcttgccc catgacattt aagactatat tttttaaagc cttagaacag actttgaagt
     2401 cgcatcctaa aacaaatccc gtggcgattg atcttcctgc gggcaatggc gaaacttctc
     2461 aatttctttt aaacaatgga tacgccgttc atgcctttga tcttttcccc gaatt
//
LOCUS       DATBTE010000154         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_906909, whole genome shotgun sequence.
ACCESSION   DATBTE010000154 DATBTE010000000
VERSION     DATBTE010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906909"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..847)
                     /locus_tag="VIG33_05845"
     CDS             complement(47..847)
                     /locus_tag="VIG33_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270392.1"
                     /translation="MKKWMLLTNILTLLVLTACQGSKETVVEKHFVANEGNNLPVPPD
                     KVEGGIDGGGGGNGLERKPLESFRIDLTQVSSFQRLKTKVIDKLVPRFPKLAADLIHV
                     VEERSWYLIPTELRNLPASKIGVSFKTDQIALQKLKEIWINDLIFAKMEAVDQETLLL
                     HELLMGVRLLEFANFLDQCLVNISILKITEATQEKYKEQRQLCFKKYRNAADIGDSIG
                     LGKDIKLEDYDYDTLRDFTNLLLTKTETFDVQELEDQMAIRGFRKYTP"
     gene            complement(852..1247)
                     /locus_tag="VIG33_05850"
     CDS             complement(852..1247)
                     /locus_tag="VIG33_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270393.1"
                     /translation="MKTMGSYFLKILGMVTLTMPLALEASAEMTVTNIQIQHCTSQGD
                     LCIKVKSPKAKVSMVKPIYFMKDIEIEMGNPKGPDSQLTDKSSKTFVQGYLDLDNNQL
                     VLQSIDKKATLTEEVYNLTTLQKQVFVMR"
     gene            complement(1257..2498)
                     /locus_tag="VIG33_05855"
     CDS             complement(1257..2498)
                     /locus_tag="VIG33_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270394.1"
                     /translation="MKTILKTTILATSLIIALTACSEKDKDNTTNVHADASMKADELA
                     DAGEQLVSPYTFMLSDKVFDMALEKDPSNKKAQFYKAFVKRMMVLKGIGKRIAPLMKK
                     GTAKQQADYEKAKTDLPDSPLKSFLLDGPEDIKSYSQLVSVIADYNSALNDFRKFLKL
                     NAGTLDLTLNLNPHIFEKEINDQAAHSCSWTQSPEGAIDVQCETAGIAQKKVNTADLI
                     ALQQMTGGEMLYWSLYTAYDLSTVEEIAKNERLKTMPPQEVLAYYKSLPSLGKLNKNQ
                     TLSLIPELGSDFSASVKWAQQYQGRLCPKGVKVPNQRRGFLFKDGVCVETTNEVNKAL
                     AMLDQMVSGIYTQPILDEQGQQVDQIRVNLVGFLKNPPQDLKAFLPESIDQNGKTPQW
                     SDNTLGGLFPDGDIQKLRKEK"
BASE COUNT          724 a          622 c          635 g          928 t
ORIGIN      
        1 cagcttctga aacgacccgc tgcttgaata tagattggtt tgagttttag ggagtatatt
       61 ttctaaaacc gcggatcgcc atttggtctt caagctcttg cacgtcaaaa gtttcggtct
      121 tggttaacag aagattcgta aaatcacgga gagtgtcgta atcataatct tcaagtttaa
      181 tatcttttcc caaccctatt gaatcgccta tgtcagcggc gtttcggtat tttttgaagc
      241 acaactggcg ctgttcctta tacttttcct gtgtagcctc ggtgattttt aaaattgaaa
      301 tattcaccag acactgatct aaaaaatttg caaactccag caaacgaacc cccatcagaa
      361 gctcatgcaa gagcagggtt tcttggtcta cggcctccat ctttgcaaaa atcaaatcat
      421 tgatccaaat ttcttttaat ttttgcaggg cgatttgatc cgttttaaaa ctcactccga
      481 tttttgaagc gggaagattt ctcagctcag tggggattaa ataccatgag cgttcctcca
      541 caacatggat aagatcggcc gccagtttcg gaaagcgcgg cacgagctta tcaatcactt
      601 tggtcttgag tctttgaaac gaggaaacct gggtcagatc aatgcggaaa ctttccagag
      661 gcttgcgttc caagccgttt ccgccgcctc cgccgtcaat tccgccttcg actttgtctg
      721 gggggaccgg caagttatta ccctcgttgg caacgaaatg cttctccacg acggtttctt
      781 tcgagccttg gcaggccgtc aaaaccagga gagttaaaat gttggtcaat agcatccact
      841 ttttcatttt attatctcat gacgaaaact tgtttttgca gcgtggtgag attgtaaact
      901 tcttccgtga gagtcgcttt tttatctatg ctttgcaaaa caagttggtt gttatccaaa
      961 tccaaatagc cctgaacgaa ggttttcgat gacttatccg taagttgaga gtcaggtccc
     1021 tttggattac ccatttcaat ttcgatatct ttcatgaaat aaataggctt taccatactc
     1081 actttcgcct ttggagactt cacttttatg caaaggtctc cttggcttgt gcagtgctgg
     1141 atctggatat ttgtaaccgt catttcggcc gaggcttcca gagccagagg catggtgaga
     1201 gtcaccatgc cgagtatttt taagaagtaa gaacccattg ttttcacttt aagatcctat
     1261 ttttcttttc tcagtttttg aatatcccca tctgggaata aaccaccgag tgtattgtct
     1321 gaccactggg gtgttttgcc attctgatca atagattcag gtaagaaggc ttttaaatct
     1381 tgcgggggat ttttcaggaa gcccacaagg ttgacacgga tttgatccac ttgttgacct
     1441 tgctcatcca aaatcggttg cgtgtaaata ccactgacca tctgatcaag catagctaag
     1501 gctttgttga cttcgttggt ggtttctacg caaactccat ctttgaacaa gaaacctctg
     1561 cgttggttag gaaccttgac tcctttaggg cacaagcggc cttggtattg ttgcgcccat
     1621 tttacagagg cactgaagtc gctgccgagc tcgggaatca aactcaaagt ttggttcttg
     1681 ttcagttttc ctaggctcgg aagactttta tagtaagcca aaacttcttg aggcggcatg
     1741 gtcttcaagc gttcattctt agcgatttct tccactgtgg aaaggtcata ggccgtatac
     1801 agggaccagt agagcatctc accaccggtc atttgttgca gagcaatcag gtccgctgtg
     1861 ttcactttct tttgcgcaat tccggctgtt tcacattgaa cgtcgatggc cccttcagga
     1921 ctttgagtcc agctgcaaga gtgcgccgcc tggtcgttga tttctttttc aaagatatgc
     1981 gggtttaagt tcaaagtgag gtctagagtc ccagcattta attttaggaa ttttctaaaa
     2041 tcattgaggg ccgagttgta gtcggcaata accgagacta attggctgta agacttaatg
     2101 tcttcggggc cgtctaaaag gaaggatttc agaggggagt ccggcaaatc tgtttttgct
     2161 ttctcatagt ccgcttgttg tttcgcggta cctttcttca tcaagggagc aatacgcttg
     2221 ccgattcctt taaggaccat catgcgttta acgaaggctt tgtagaattg agcttttttg
     2281 ttggaaggat ctttttctaa agccatatca aagactttat cagaaagcat aaaagtataa
     2341 gggcttacta actgttcgcc cgcatccgcc aactcatccg ccttcattga ggcatcggca
     2401 tggacattgg tcgtattgtc cttgtctttt tcagaacaag ctgtcagcgc tatgattaaa
     2461 cttgtggcta agattgtggt ttttaaaatc gtcttcataa tcatctcctt ggtttgttaa
     2521 gcagctttta acgcaccgct ttgttgggtg agttatagtc cgtttaacaa aacaaccata
     2581 gattttgtta gtaaactgtt tcagtaaata ttaaccattt ggttaatatt ttaggctcaa
     2641 aaccaactgg agttgttttc gagcctagga agcttcctac aaggagcatg cccgcttgaa
     2701 ataggtttag atcgtttgca gtggcttagg attcgacata gcgacgagga agggcccaat
     2761 acgtcgaagc ctctccgttt tttagaatgg ggtagtttgt tccaactgat gatagaaaat
     2821 atgccagacc taggtctatt ggtttttatt aaaacaagtc tttattctat ccgaatgttt
     2881 tcattggact tctggttgta ctcaaacag
//
LOCUS       DATBTE010000155         9612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1523441, whole genome shotgun sequence.
ACCESSION   DATBTE010000155 DATBTE010000000
VERSION     DATBTE010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9612
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523441"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VIG33_05860"
     CDS             <1..330
                     /locus_tag="VIG33_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270395.1"
                     /translation="MGIDPITQRPVYSEGLLQLSYQDSKAYPFCDEFNWTRDKHLHPT
                     DPRKTILDPYKNLHCGVKILAQQIRNRDRITLSSGVYWSVLKLGGKYNKIKQIAALTR
                     KMPGCMN"
     gene            335..838
                     /gene="queF"
                     /locus_tag="VIG33_05865"
     CDS             335..838
                     /gene="queF"
                     /locus_tag="VIG33_05865"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEY8270396.1"
                     /translation="MKKSRQKELKNFALGSEKTAYPTTYSPEILEAFDNKNPGRVAWT
                     TFVCTEFTSLCPKTHQPDFAKIFINYIAAEKMVESKSLKLYLFSYRNHGDFHEDCVQK
                     ICDDLVKLMKPKYLEVIGEFTPRGGIAIYPFASYANKEKTFQQLWSHRLAEYAPGKYS
                     MELSKLY"
     gene            1007..2338
                     /locus_tag="VIG33_05870"
     CDS             1007..2338
                     /locus_tag="VIG33_05870"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEY8270397.1"
                     /translation="MDLSKLVLNALGLQHEDVKIESFEVDQQSLTACVKIRQIRENCR
                     CPKCHEPLYGVKQWRRRELWGVPLGALQKVKIIFFQLQAACGRCLKLPLAEARFIHPR
                     FKQMTAAFAEYVGRWMEETTIAAVERMTECPGMSLWRLDQWRMKTMKKMFEIPTDMPM
                     KFASADEVHMRTIKPKDARFDKSKWDKKFITNLVSCDLAKVVANASGRSSRSLTSCLR
                     QLPDHVRENIKFIAVDMHEGYIKAATKLCPNAKVTVDRFHVAEALNRAFDELRKQEFD
                     KAKQANDAFQSEMLKPSKKYVLVARKKDLSKKDQSQFERLIQVNQNISNALILIDYFH
                     SILDKHEVKDFREGLKLWEELVTASKITQFEKFLETVKKHQTRIEAYISSKLTTAISE
                     GINNKIKVLKRVGYTYTNEESFKNKILQRCGFINSRNLNTNGWYWHVPTPH"
     gene            2499..3329
                     /locus_tag="VIG33_05875"
     CDS             2499..3329
                     /locus_tag="VIG33_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270398.1"
                     /translation="MFNKWALILAGLFFTQQIVAKTANEFSSNTESAAPCIECSRPTN
                     STFESIRIFAENLKESTRGALDIEYIFDKMHYSNKCDSFAADGEMNKWGNTITNELRR
                     NKGNISSSEEEDTRRYKPLLSQGPKDIKKYCTNFQAMTTPEREALYVLILTAMAHYES
                     SCNFREKAPGPNGTAAGLLQLHKGREQKYSSGCRRGDSARPERTLICGLSMLNDQIER
                     GENLFSRDSYWEVLRPQGRSKKAGKIMAAIKKFPACGAMDTPTIANSKHKSKTRIAQK
                     "
     gene            complement(3349..5010)
                     /locus_tag="VIG33_05880"
     CDS             complement(3349..5010)
                     /locus_tag="VIG33_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY8270399.1"
                     /translation="MPKAAKPFIFPNMIHLSNITKQYGSKILYRNASFQINDGEKIGL
                     VGPNGAGKTTIFRVITGEEGCDGLVSKSDKTVIGYFSQNIEDMKGRSALEEVKSAAGK
                     IPELATKLKMLEQRLEDSAVTPISDEAMEKLLESYGEVQAEFERLGGYDLDTRAAEIL
                     TGLGIGPEDYHRPTESFSGGWKMRIALAKILVLNPEVLLMDEPTNHLDLESIIWLEEW
                     LTNFKGALLMTSHDRDFLNRLVSKVVEVANKTITTYGGNYDFYEKEKAIRLEQLIAAA
                     KRQDDMLAKEEEFIARFAARASHAAQVQSRVKKLEKIDRIEIPDEEKAIKFEWAEPPR
                     GGDEVVKFEGLTKTWRRDDGKEKLVFANANALVKRMDRVAVVGVNGAGKSTLLKIITG
                     QTEASGGQLALGASISLGYFSQNSLDVLDPHATIMEEVHSRIPNSTIGFVRNLLGAFK
                     FSGDDADKKISILSGGEKSRVVLATILAKPVNFLVLDEPTNHLDIVSREVLLDAIKTF
                     PGTVMIVSHDRHFLREVTTRVFELDKNHLVTYDGTWDYYLSKRPS"
     gene            5101..6942
                     /locus_tag="VIG33_05885"
     CDS             5101..6942
                     /locus_tag="VIG33_05885"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8270400.1"
                     /translation="MHSNSDSHSDAAVTPISSSFKSYGLSDALIKAMDEMGFTSPTPI
                     QQKAIPMLLGEAQDMIGLASTGTGKTAAFGIPLIEKIDASSKSVQGLVLSPTRELAMQ
                     VSDQLQKLGKYKGVRVVTIYGGSSYRTQLEGVKRGAHIVVATPGRLVDFLEQKAVALN
                     NVQTLVLDEADEMISMGFKDDLEFILKNTHNEDAAASGRAACKTWLFSATMSRDIRRV
                     ADTYLEKPQTVEVAKTQGMSELVKQIYFTVKDEHKNEVISRLLQMHDDFHGIIFCQTK
                     MEVIELEDKLQKRGFSVASLHGDKQQREREITLKKFRTGETKVLVATDVAARGLDVKD
                     LTHVINHSLPWDVESYIHRIGRTARNGKEGSAISLINPRQVSSLRRLQNVTKKNLEKG
                     KVPTADEVALLRLQKFMAKLAVVATEGRKYKIALEMFTELVQKDESGLLNLASPELLA
                     RVITALHPEMLAEKDYDLDFMKGGLEQRPARRDEGSREGRTLSGGYRNDSRGGGYRSG
                     GNSRGGGYSDRPRSSGYAASAPAERPRREEIRREEARETFAPRRDRGPRFDGFDFGNG
                     GSASRGERSERPPRRTTERAAERPGDHPGIKRHRSGGIKRRPASDNA"
     gene            complement(7034..7498)
                     /locus_tag="VIG33_05890"
     CDS             complement(7034..7498)
                     /locus_tag="VIG33_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8270401.1"
                     /translation="MITPVKKVMTKKLLTIPVGTSLFEAHEVMKENRIRHLPVVDATD
                     DVLGILSQRDLNAIPDSKNIPVEWMMSSPVEYVDQNISLRKAILLMLQKKISCLLIAD
                     EKENAVGIVTTDDLLWHLANLLSDETENKPLLSFIDKQTIGEVANELSMMGI"
     gene            complement(7645..8442)
                     /locus_tag="VIG33_05895"
     CDS             complement(7645..8442)
                     /locus_tag="VIG33_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270402.1"
                     /translation="MISKDNAPNYAALKKDGKLTGIMIVGSNLSSGHSYVVDEYLTYY
                     QNQGFEFKPTRPIEAISFFQKSVETGEADYLIKEAHSDGDERNLFRANKYGKLFEGSL
                     IKKDGTKEVIYLIAPDDEKKDSKHISNQEFGSWIRSRGKDQPLIYLNASCNSERKVIS
                     EIAAAHSSNFIPIPSATSVLTFTDSDNSGEKQFLQSLRNEKSYEDIRASIQKTKAYQK
                     GEDHFLFPDEKDYDDKIRKNLIMNLDFEVKIKDAAGHELQIDESIDH"
     gene            complement(8457..>9612)
                     /locus_tag="VIG33_05900"
     CDS             complement(8457..>9612)
                     /locus_tag="VIG33_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270403.1"
                     /translation="LPMIRLILLILPNGLRLIFNPKTGKSRSFQKGDSINFDSKLEKW
                     IKLESFPQTIDTVCLPTSIEVIDDDCNCLIQNADVASPLGSNLEEVQDLALTAMCQSE
                     TFNPTDWSKLTPPITKGQISKKQAQLYLKRFQKFGGYSDTHLPILTAILKSDLVETHP
                     DLVNQTLKALALSRSETVQAFYHLLKIDQRMPSKYSLDESNKCKSGFEKNLETSKIEY
                     LKSVSGNIERKTTTEDWLEFKPFKEELQKLPKEQRDAIVDGIAESLSRNAMASPELNG
                     VFQSKLYYFSKKWALNLVGDTAKSATDLGMAISNGKATPIVLGSGDLPNASSSKYEDS
                     INSTDLYGFQFKVMDPISIPKDSKVGEKKSKKISGSMTARNTPQAPRAPS"
BASE COUNT         2700 a         2222 c         2092 g         2598 t
ORIGIN      
        1 atgggaattg atcctataac ccaaagaccc gtctacagtg aaggtctact gcaactttcc
       61 tatcaggaca gcaaagccta tcccttctgc gatgaattca attggacacg ggataagcat
      121 ttgcacccga cggatccccg caagaccatt ttggatccct ataaaaacct tcattgtggg
      181 gtaaaaatac ttgcccaaca gattagaaat agggatcgta tcacgctctc tagcggtgtt
      241 tattggtctg tccttaaact cggtggcaaa tacaataaga ttaaacaaat tgccgcattg
      301 accagaaaga tgcccggatg tatgaactga cccaatgaaa aaaagccgac aaaaagaact
      361 taaaaatttc gctctagggt ctgaaaaaac cgcttaccct accacgtatt ctccggaaat
      421 tctggaggct tttgacaata aaaatccagg ccgagtggcc tggaccactt ttgtctgtac
      481 cgagttcact tctctttgtc ctaagactca ccaaccggat tttgcaaaga tcttcatcaa
      541 ctacatcgcc gccgaaaaga tggtggagtc caaatcttta aaactctatc tttttagcta
      601 tcgcaaccat ggggatttcc acgaggattg tgtgcaaaaa atctgcgatg atctggtgaa
      661 gttgatgaaa ccaaaatatc ttgaagtgat cggtgaattc accccccggg gcggaattgc
      721 catttatcct ttcgcgagtt atgccaacaa agagaaaact ttccagcaac tgtggtctca
      781 ccgtctagcg gaatacgccc ctgggaagta cagcatggaa ctttcaaaac tctactgatt
      841 tcaaggctta aacataagct gtctcggcaa gggctgtctg gctctctaac tttgagtagg
      901 gcgccgcaaa aagtgtcaca tttaaaaatg aaagaaattg tccatccaag gttcctctgg
      961 tttgattttt ttacgacaaa aaaacaacta tgaggagaca gcaaggatgg acctgtcaaa
     1021 attagtatta aatgccttag gattgcaaca cgaagatgta aaaattgagt ctttcgaggt
     1081 cgatcaacaa agtttaaccg cctgcgtgaa gattcgccag atccgggaga actgccgctg
     1141 tcccaaatgc catgagcccc tttacggagt caagcaatgg cgcagacgag agttgtgggg
     1201 cgtccccctc ggggccttgc aaaaggttaa aatcatcttc tttcagctgc aggcggcttg
     1261 tggccgatgc ctcaaacttc cacttgccga ggctcgattt atccatccaa gattcaagca
     1321 aatgacagcg gcctttgccg agtacgtggg gcgatggatg gaagagacga ccatcgcagc
     1381 cgtggaacga atgactgaat gccccggcat gagtctttgg cgacttgatc agtggcgaat
     1441 gaaaacgatg aagaaaatgt tcgaaattcc aacagacatg ccgatgaagt ttgccagtgc
     1501 cgacgaggtt catatgcgca caatcaagcc caaagacgca agatttgata agtcaaagtg
     1561 ggataaaaaa tttatcacta acctcgtcag ttgcgatctt gcgaaagtcg ttgcaaatgc
     1621 cagtggtcgc agcagcaggt cactcaccag ttgtttaaga cagcttcctg accatgttcg
     1681 tgaaaacatt aagttcatag ctgttgatat gcacgaagga tatatcaagg ctgcaacaaa
     1741 actttgtccg aatgctaaag taaccgtgga ccgttttcac gtggctgagg ctttgaatcg
     1801 ggcttttgat gagcttcgca aacaggagtt cgacaaggca aaacaagcca atgatgcatt
     1861 ccaatctgaa atgctgaagc catcaaaaaa gtacgtcctt gttgcgcgta agaaagatct
     1921 ttcaaagaag gatcagtcac aatttgagcg cctcattcaa gtaaatcaaa acatcagcaa
     1981 tgccttaatc ctcattgatt acttccacag catactggac aaacacgaag ttaaagactt
     2041 tcgtgagggt ctgaagctct gggaggaact cgtcacggcc tctaagatta cacagtttga
     2101 aaagttcctt gagactgtaa agaagcacca aacacggatc gaggcctaca tatcgtcaaa
     2161 acttacgaca gcaatttctg aggggatcaa taacaaaatc aaggtgctca aaagagtggg
     2221 atacacctac accaatgaag aatcctttaa aaataagatc ttacaacggt gtggattcat
     2281 aaactcaaga aacttaaata caaatggatg gtattggcat gtacctacac cccactaatt
     2341 gatagaaagg cgggctgtct caaattgaga cagccctttt ttgtcgtccc ctgcatagtt
     2401 tacaaacatc tcaataccct tcatttcgaa atgagacaag ttagagcacc ggcatcctct
     2461 ttgctttgta aggtcctata ggcagaaagg acatagctat gtttaataag tgggctctca
     2521 tactggcagg actatttttt acacagcaaa tagtggccaa aacagcaaat gaattcagct
     2581 ccaatactga gagcgcggcg ccctgtattg aatgcagcag accaaccaat agtacatttg
     2641 agtctataag aatctttgcc gagaacctca aagagagcac gcgtggagcc ctcgatatcg
     2701 aatatatttt tgataaaatg cattacagca ataaatgtga cagcttcgcc gccgatggcg
     2761 agatgaataa atggggcaat accatcacta atgaactcag acgtaacaaa ggtaacattt
     2821 cctcttcaga agaagaagat actagaaggt ataagcccct cctttcgcag ggtcctaaag
     2881 atattaagaa gtattgtaca aactttcagg caatgacgac acctgaaagg gaagctctgt
     2941 atgttcttat tctcactgca atggctcact atgaaagctc ctgcaatttc cgcgagaagg
     3001 cccccggccc taatggaaca gcggcgggac ttttgcagct ccacaaaggt cgtgagcaaa
     3061 aatactcttc cggatgtcgg cggggcgact ctgcaagacc agaaagaacc ttgatttgcg
     3121 gcctatcaat gcttaacgat cagatagaac gcggcgaaaa tctctttagc cgtgactctt
     3181 actgggaagt tctacgcccc caaggcagat ctaaaaaagc ggggaaaatc atggctgcca
     3241 taaaaaagtt tccagcctgt ggagccatgg acactcccac tatcgccaac tcaaagcata
     3301 agagtaaaac tcgtatagcc caaaagtagc ttcctcaatg aggccccgtt aagagggacg
     3361 ctttgaaaga taatagtccc aagttccatc gtaggtcaca agatggttct tatcgagttc
     3421 aaatacccgg gtcgtcactt cacgcaagaa atgccgatca tggcttacga tcatcaccgt
     3481 gcctggaaag gttttaatcg catcgagcaa aacttcccgc gagacgatgt ccaagtgatt
     3541 cgtcggctca tccagaacta gaaagttcac cggctttgcc aaaatagtag caagaaccac
     3601 gcgacttttc tcaccgcccg ataaaataga aattttctta tccgcatcgt ctccactgaa
     3661 tttaaatgcg ccaagcagat ttctgacaaa accaatggtc gaattcggaa tgcgcgaatg
     3721 cacctcttcc ataatagtgg catgggggtc cagcacgtcc aaagagttct ggctaaaata
     3781 gcccaaagag atagaagctc ccagagcaag ttgccccccc gaggcttccg tttgaccagt
     3841 aataattttt agcaaagtgg atttaccagc tccattgact ccgaccacgg ccacgcggtc
     3901 cattcgcttc accagagcgt tggcatttgc gaagacgagc ttctctttgc catcatcccg
     3961 tctccaagtt ttagtcagac cttcaaactt caccacttca tcacctccac gaggaggctc
     4021 cgcccattca aacttgatag ctttttcttc atcgggaatt tcgatgcgat caattttttc
     4081 aagtttcttc acccgggact gaacttgggc cgcgtgagag gcacgcgctg cgaagcgagc
     4141 aataaattct tcctcttttg ccagcatatc atcctggcgc ttcgccgcag ctatcagctg
     4201 ttccagacga atggcttttt ctttttcata gaaatcgtaa ttcccaccat aggtagtgat
     4261 tgttttattg gcaacttcaa caacctttga taccagacga tttaagaaat ctcgatcatg
     4321 gctggtcatc aaaagagcgc ccttgaaatt ggttaaccac tcttcaagcc atataataga
     4381 ttccagatcc aagtgattcg taggctcatc catcaagagg acttccgggt tcaaaaccag
     4441 gattttagcc aacgcaatcc gcatcttcca gccaccgcta aagctttctg tcgggcgatg
     4501 ataatcctca gggccaatcc ccagaccggt taaaatctca gccgcccgag tgtccaaatc
     4561 gtagccccca agccgttcaa attcggcctg aacctcgcca tagctctcca ggagcttctc
     4621 catggcctca tcgctgatag gagtcaccgc agagtcttcg aggcgctgtt caagcatttt
     4681 caatttagtg gccagctcag gaatcttacc agcagcggat ttcacctctt ccagcgccga
     4741 acgacccttc atgtcttcga tattttgcga aaaatagcct atcacagttt tatcggactt
     4801 ggacaccagg ccgtcgcagc cctcttcgcc cgtaatcact ctgaaaatcg tggtttttcc
     4861 cgcaccattc ggaccgacga ggccaatttt ttcaccatcg ttgatctgaa aagacgcatt
     4921 cctgtacaga atcttgctac cgtattgttt agttatattt gagagatgaa tcatgttggg
     4981 gaagataaag ggtttcgcgg ctttaggcaa gacagcgctg aataaaatac agccttcatt
     5041 attttaaggc gaaaggggta aaaatagttt aaattggcgt tttccatgcg aaaggttccc
     5101 atgcattcta attctgactc ccattccgat gctgctgtca ctccgatatc ttcttccttt
     5161 aaatcctatg gactgagcga cgcgcttata aaagccatgg atgagatggg ctttacttcg
     5221 cctactccca ttcaacaaaa agcgatcccc atgcttttag gcgaagccca agacatgatc
     5281 ggacttgcct ctaccggcac aggaaaaact gcggcctttg gtattcccct cattgaaaaa
     5341 atcgatgcca gttctaaaag cgtccaagga cttgtcctaa gtcccactcg agaactggct
     5401 atgcaggtct ctgatcaatt gcaaaagctt ggtaaatata aaggcgttcg cgtggtcacc
     5461 atctacggtg gttctagtta tcgcactcaa ttagaaggcg ttaaaagagg cgcgcatatc
     5521 gtggtggcga cccctggccg tctggtggat ttcttagaac aaaaagcagt ggcgctgaat
     5581 aatgtccaaa cgctggtttt ggatgaagcc gatgaaatga tcagcatggg tttcaaagat
     5641 gatttggaat tcattttgaa aaacactcat aatgaagacg cagcggcttc gggccgtgca
     5701 gcttgcaaaa cctggttatt ttctgccacc atgagtcgcg atatccgtcg cgtagccgat
     5761 acctatctgg aaaaaccaca aacagtggag gtcgcaaaga cccaaggtat gtctgagctg
     5821 gttaaacaaa tttactttac tgttaaagat gaacataaaa acgaagtgat cagccgtctt
     5881 ttacaaatgc atgatgattt ccatggcatt atcttttgcc aaacaaaaat ggaagtgatc
     5941 gagcttgaag acaagctgca aaagcgtggt ttctctgtcg cttctctgca cggagataaa
     6001 caacagcgtg agcgtgaaat cactcttaaa aaattccgca ccggcgaaac caaagttttg
     6061 gtggctacgg acgtcgccgc tcgcggttta gatgtgaagg acctcaccca tgtgatcaat
     6121 cactctttgc cttgggatgt ggaatcttat attcaccgta tcggccgtac tgcacgtaat
     6181 ggtaaagaag gatccgctat cagtttgatc aatccacgcc aggtcagctc tcttcgtcgt
     6241 ttacaaaacg tcactaagaa gaatttagaa aaaggcaaag ttccaacagc ggatgaagtg
     6301 gctttgcttc gcctgcaaaa attcatggct aaattggctg tggtggcgac cgaagggcgt
     6361 aagtataaaa ttgctttgga gatgttcacg gaactcgtgc aaaaagatga gtctggttta
     6421 ttgaacctgg cttctccgga attgttggcc cgcgtgatca ccgctcttca tcctgagatg
     6481 ttggcggaaa aagattatga tttggatttt atgaagggtg gacttgaaca gcgtccagcc
     6541 cgtcgtgacg aaggcagccg cgaaggtcgt acattgagtg gcggctaccg caacgattcc
     6601 cgcggcggcg gatatcgtag tggtggcaac agccggggtg gtggttattc cgatcgtcct
     6661 cgtagcagtg gctacgcggc ctcagctcct gcagagcgac ctcgtcgtga agaaattcgc
     6721 agagaagagg ctcgcgaaac cttcgctcct cgccgcgatc gcggcccacg ctttgatggt
     6781 ttcgatttcg gtaatggcgg cagcgccagc cgcggtgaac gcagcgagcg tcctccgcgc
     6841 cgtacaacag aacgtgcagc agagcgcccg ggagatcacc caggaatcaa acgccaccgc
     6901 agcggcggca tcaaacgccg tcccgcaagc gataacgctt aatcgtctta ataaagcgag
     6961 ctccacgagt ttttctaaac tcgggagttc gctatttttt tgtcaaaaat tcacttatca
     7021 aggtatgaaa tcctcaaatt cccatcatcg ataattcatt ggctacttcg ccaatggttt
     7081 gcttatctat gaaagataaa agcggtttat tttcggtctc atcgctgaga agattcgcca
     7141 aatgccaaag cagatcatcc gtcgtgacaa tgcccacggc gttttctttc tcatcagcaa
     7201 tgagcagaca agatattttc ttttgcagca tcaacaaaat agcttttcga agagagatat
     7261 tctggtccac atactccacg ggagaactca tcatccactc tacaggaata tttttggaat
     7321 ccggaatagc attgaggtct ctttgggaaa gaatacctaa tacgtcatcc gttgcgtcga
     7381 cgaccggaag gtgacggatt cgattttctt tcatcacctc atgagcctca aaaagagacg
     7441 ttccaaccgg gatcgttaat agctttttgg tcataacttt tttgacaggt gtgatcatct
     7501 tatccccctt catcatgtag atattactgg gggttgcaaa aacgaggcct gagctaattc
     7561 aagctgagga atttttcgag gtattttgtc tcaaaaaata aataacgcaa atagcaaatg
     7621 tcccgactta aggatcagcg aaccttaatg gtcgatgctt tcatcaattt gaagctcgtg
     7681 tcccgcggca tcttttattt taacttcaaa atcaagattc atgatcaggt tttttctgat
     7741 tttatcatca taatcctttt catcagggaa aaggaaatga tcttccccct tctgataggc
     7801 tttagttttt tgaatacttg ccctgatgtc ctcatagctt ttttcatttc tgaggctttg
     7861 caaaaattgt ttttcaccac tgttatccga atcagtaaag gtcaatactg aggtcgcgga
     7921 aggaattgga ataaaatttg aggaatgagc cgccgctatt tcagaaatga cctttctctc
     7981 agaattgcag ctggcattta aataaataag aggctgatcc tttcctctgc ttctgatcca
     8041 actgccaaac tcttgattgg atatatgctt tgagtccttt ttttcgtcat ctggggcaat
     8101 caaatagatc acttctttgg tgccatcttt ttttataaga cttccttcaa aaagttttcc
     8161 atatttattg gctctgaata aattcctctc atctccgtcg gagtgggctt cttttataag
     8221 atagtctgct tcgccagttt ccacggattt ttggaaaaat gaaatggcct caattggcct
     8281 tgtgggttta aattcaaatc cttgattttg atagtaggtc agatattcgt caacaacgta
     8341 tgaatgccct gaacttaagt tactgcccac aatcatgata cctgtgagtt ttccatcttt
     8401 tttcaaagcc gcataatttg gagcattgtc ttttgatatc atgttgtcga gaggctctaa
     8461 gacggtgctc gtggtgcttg cggtgtattt ctggccgtca tgctgccaga tatcttcttt
     8521 gatttcttct ctcccacttt agagtctttt ggtattgaga ttggatccat caccttgaat
     8581 tgaaaaccat ataaatctgt ggagtttatc gagtcttcat attttgacga agaagcattt
     8641 ggaagatcgc ctgaccccag gactataggc gtagcctttc catttgaaat ggccataccc
     8701 aggtccgtcg ctgactttgc agtatctccg actaaattta aggcccactt tttagagaaa
     8761 taatacagct tactctggaa aacgccattt agttccggtg atgccattgc attgcgtgaa
     8821 aggctttcgg caatcccatc cacgatggca tctctttgtt cctttggaag tttttggagc
     8881 tcttccttaa acggtttgaa ttctagccaa tcttcagtcg tcgtctttct ttcaatattc
     8941 cctgaaactg atttcaaata ttcaatttta cttgtttcaa gatttttctc aaatcctgat
     9001 ttgcatttat tggattcatc aaggctatat tttgaaggca ttcgctgatc aatttttaaa
     9061 agatgataaa aagcctgaac agtttcagaa cggcttaagg caagagcctt gagtgtttgg
     9121 tttacaagat ctggatgtgt ttccactaaa tctgatttca aaattgcagt gagaatgggg
     9181 aggtgagtat cagaataacc cccaaatttc tggaatctct ttaaatagag ttgtgcttgc
     9241 ttcttagaaa tttgaccttt tgtaattgga ggcgtcagtt tactccaatc cgtgggatta
     9301 aaagtttcag actggcacat ggctgttaag gctaagtctt gcacctcctc cagattagag
     9361 ccgaggggtg aggcaacatc tgcattttga ataaggcaat tgcaatcatc atcaatgact
     9421 tcaattgaag ttggtaagca gaccgtgtct attgtctgcg ggaaggattc tagtttaatc
     9481 catttctcaa gtttactatc aaagttgatg gagtcaccct tttgaaatga ccggctttta
     9541 ccagtctttg gattaaagat caggcgaagc ccattgggaa ggatcaataa aattaaccgg
     9601 atcattggca ac
//
LOCUS       DATBTE010000156         1804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_88627, whole genome shotgun sequence.
ACCESSION   DATBTE010000156 DATBTE010000000
VERSION     DATBTE010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88627"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VIG33_05905"
     CDS             complement(<1..767)
                     /locus_tag="VIG33_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270404.1"
                     /translation="MGILCRRTDLLVFSHLRWDFVYQRPQHLMSRFAKFRKVFFIEEP
                     AFGLCETPTLHIRSSVEGVQVVTPHFPHPLEPQIQELALSHFIDDLIEDENLKEYSLW
                     YYTPMALPFSRHLNPYSIVYDCMDELSLFNGCPPALIELEKELFAKADVVFTGGYSLY
                     EAKQHHHHNIHPMPSSIDHAHFSSAKLVTVDPPDQALIPHPRLGFFGVIDERMDLDLL
                     AQMADLRPCWHFIMIGPVVKIDEALLPQRENIHYLGR"
     gene            963..>1804
                     /locus_tag="VIG33_05910"
     CDS             963..>1804
                     /locus_tag="VIG33_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HEY8270405.1"
                     /translation="MFTNTLEDSEKNYPRKLLRRNNWSNLNGTWQFRLDHEKKYNRPN
                     QIPDWPLTIQVPFAPESPASGIGDNDFHSRVWYQRTFEFYKTDMRVILHFGAVDYEAR
                     VWINNHYLGHHEGGHTPFSFDVTDLLNSGGDQIITVRADDDPLDLTKPRGKQDWQRHP
                     HSIWYPRTTGIWQTVWMEEVAQTYVERLRCTPMLERWEISIEAFIEGTYKEGLQLEVK
                     VKSGSKLLARDHYEVVNKEVHRKIAMSDPGIDDYRNELLWSPEKPTLIDIEIKLIDEG
                     KVLD"
BASE COUNT          581 a          369 c          394 g          460 t
ORIGIN      
        1 tttcttccaa gataatgaat attttcccgt tgcggcagca gcgcctcatc aatcttaacg
       61 accggaccaa tcataatgaa atgccaacaa ggtcgcagat ccgccatctg agctaaaaga
      121 tccaaatcca tgcgttcatc aatgactcca aaaaaaccga ggcgaggatg cggaataagg
      181 gcttgatcag ggggatccac tgtcactaac tttgcactgg aaaaatgggc atggtcgata
      241 ctgctgggca ttggatgaat gttgtgatga tggtgttgtt tagcctcata caatgaatat
      301 cctccggtaa acacaacatc cgcttttgca aagagctctt tttccagttc aattaaggcg
      361 ggcggacacc cattaaataa agacaattca tccatgcaat cataaacaat ggagtaagga
      421 tttagatgtc tggaaaacgg caatgccatg ggcgtgtaat accataaaga atattctttc
      481 aggttttcat cctcaattaa gtcatctata aaatgagaaa gcgccagctc ttgtatttgg
      541 ggctccagag ggtggggaaa gtgcggggtg acaacttgga ctccttccac agaggaacga
      601 atgtgcaaag tcggagtttc gcataagcca aaagcgggtt cctcaataaa aaaaactttt
      661 ctgaatttag cgaaacggga catgagatgc tgtgggcgct ggtaaacaaa atcccaacga
      721 agatgagaaa aaaccaataa atctgtgcgt cgacaaagaa ttcccattca gcataactcc
      781 tttattaaaa ataattttaa aatccatttt aatctaaaaa aaattttgcc aaaataacaa
      841 gcggcgctat ttatatatag ttatacatag ccacttaact actaactctt tgagataaaa
      901 actcaaaaaa gctgccttct tagaactaaa aatatttctc aaattaaaat ggagagttcc
      961 ggatgtttac aaatactttg gaagattcag agaaaaatta ccccagaaaa ttacttagac
     1021 gtaataattg gtctaatctc aacggaactt ggcaatttcg cttggatcat gaaaaaaaat
     1081 acaatcgacc caaccagatc cccgactggc ccttgaccat tcaagttcca tttgcaccgg
     1141 aatctccggc gagtgggatt ggagataatg attttcattc gcgagtttgg taccagcgta
     1201 cttttgaatt ttacaaaacg gatatgagag tcatccttca ttttggcgct gtcgattatg
     1261 aggcgcgggt ttggattaac aatcattatc tgggacatca cgaaggaggt catacgccct
     1321 tcagttttga tgtcacggat ttactcaatt ctggcggaga ccaaatcatt actgtccgtg
     1381 cggatgatga tcccctggat ctgaccaaac cccggggaaa gcaggactgg caacgccatc
     1441 cgcatagcat ttggtatcca cgcactacag gaatttggca aactgtttgg atggaggaag
     1501 tcgcgcagac ctacgtggag cgattgcgct gcacacccat gcttgaacgc tgggaaatta
     1561 gcattgaggc ctttattgaa ggcacttata aagaaggcct gcagttggaa gtaaaagtaa
     1621 aaagcggcag taaattgctc gcacgagatc attacgaagt tgtaaataaa gaagtgcatc
     1681 gaaaaattgc catgtcggat ccggggattg atgactatcg aaatgagctc ttatggagtc
     1741 cggaaaaacc caccctcata gatattgaaa tcaagctcat tgacgaaggg aaagtgcttg
     1801 atca
//
LOCUS       DATBTE010000157         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1455357, whole genome shotgun sequence.
ACCESSION   DATBTE010000157 DATBTE010000000
VERSION     DATBTE010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455357"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /gene="rpoN"
                     /locus_tag="VIG33_05915"
     CDS             <1..1000
                     /gene="rpoN"
                     /locus_tag="VIG33_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEY8270406.1"
                     /translation="DLLINYIDDDGYLKTPLEQISAEEKLPIEDLEDTLSLIHEFDPP
                     GVGARDMKECLLIQAKHLEEDTHDLVNLINNHLKELEKKNYDAIAKIMGRELEEVVEM
                     CKIIYSMDPKPGRAFVSNEVQYVTPDVYVYKVGDDFVVSLNEDGLPRLKISNFYKNML
                     KGANKAEAKTHDYIQEKLRSAVWLIKSIHQRQRTIYKVAESIVKHQRSFFEKGPSYLK
                     PMILRDIANDIGMHESTVSRVTTAKYVHTPQGIYELKYFFNSAISSSDGDSMASESVK
                     LKIKDLVGKEDPKNPLSDQKIVELLKVEGIQIARRTVAKYRDVLKILPSSQRKKFF"
     gene            1032..>2487
                     /locus_tag="VIG33_05920"
     CDS             1032..>2487
                     /locus_tag="VIG33_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8270407.1"
                     /translation="MSNEFFQEGPQLKNQYTEDRLLQAYLHWRIPPQFIVNFTPHLKH
                     LGQRAVTDMLGWAEEAETNPPKHIPYDAWGKRIDFIETCRGWKNLEGVAAEEGLIATG
                     YERKYGEYSRTYQMALLYLYHPSSALYSCPLAMTDGATRAIELYGDEYLKSKPYKHLT
                     SRDPQQFWSSGQWMTERTGGSDVSGTSTIAKKVGDHYELYGTKSFTSATTSQMAMTLA
                     RIEGADPKTRGLSLFYLELRNEKNELDKIMVHRLKDKLGTKALPTAELSLQGTPARLV
                     GGEGDGVKKISSLFNITRIYNSICSISHMRRGLALAKDYSLKRAAFGKQLIDHPLHVE
                     TLMDMYVDYAASFHLVMRAVEILGKEECGKAMPEESAVLRLLTPVIKLFSGKKALSVT
                     SEVVEVFGGSGYVEDTGIPRLLRDAQVFSIWEGTTNVLSLDVLRAIEKENAFKPFMQE
                     IQGCLLAMMKEPMAGQQLKGEIQAIEKALKDLESY"
BASE COUNT          712 a          521 c          615 g          639 t
ORIGIN      
        1 cgatctttta atcaattata tcgatgatga cggttatctt aaaactcccc tggagcaaat
       61 ttccgccgaa gaaaaacttc ccattgaaga tttggaagac actttaagtc ttatccacga
      121 gtttgatcca cccggagtgg gtgcccgtga tatgaaggaa tgtttattga ttcaagcgaa
      181 gcatcttgaa gaagatactc atgatctggt gaatttgatc aataatcatc tcaaggaact
      241 tgaaaagaag aattacgatg ccatagccaa gatcatgggc cgcgaattgg aagaagttgt
      301 cgaaatgtgt aaaatcatct attcgatgga tcctaagccg ggtcgggctt ttgtcagcaa
      361 cgaagtccag tacgtgactc cggatgtgta tgtttacaaa gtcggcgacg actttgtggt
      421 ttccttgaat gaagatggac tgcctcggtt gaagatctcc aacttctata aaaacatgct
      481 caaaggtgca aacaaggctg aagcgaaaac ccatgattat atccaggaaa aattgcgttc
      541 cgcagtgtgg ttgattaagt cgattcatca gaggcaaaga accatctata aggtggccga
      601 aagtatcgtt aaacaccagc gcagtttttt tgaaaagggc ccatcttatt tgaaaccgat
      661 gattctcagg gatattgcga acgatatcgg catgcatgag tccacagtga gtcgggtgac
      721 cacggccaag tatgtccata ccccacaggg gatttatgaa ttgaaatatt ttttcaattc
      781 tgcaattagc tcttccgatg gagactcaat ggcgagcgag tcggtgaagt tgaagatcaa
      841 ggatttagtg ggcaaagagg atccaaaaaa tcccctttca gaccaaaaga ttgtggaact
      901 gctcaaagtt gaaggaatac aaattgcccg tcgaacggtt gcgaagtatc gtgatgtcct
      961 gaagattctt ccttcgagcc aacgaaaaaa gtttttctag tctagtcggt gacttcgatt
     1021 agaggtgagt catgtcgaat gaattttttc aagagggtcc tcaattaaaa aatcaatata
     1081 ccgaagatcg tctactgcaa gcctatttgc attggagaat tccgccacaa ttcatagtga
     1141 attttacccc tcacttgaag catttagggc aaagagcggt cacggacatg ctgggatggg
     1201 ctgaggaggc cgaaaccaat ccgccgaagc acattcctta cgatgcctgg ggaaaacgaa
     1261 ttgattttat tgaaacctgc agagggtgga aaaatcttga gggagtggcg gccgaagaag
     1321 ggcttattgc cacgggctac gaacgtaaat acggcgagta ttcccgaacc taccaaatgg
     1381 cattgttgta tctttatcat ccttcttcag ctctttattc ttgtcccttg gcaatgaccg
     1441 atggggccac tcgagcgata gagctctacg gcgatgagta tttaaaatca aaaccctata
     1501 agcatttgac cagtcgagat ccgcagcagt tctggtcttc aggacaatgg atgaccgaga
     1561 gaaccggcgg ctctgatgtc agtggaactt cgacgatcgc aaagaaagtt ggcgaccatt
     1621 atgagcttta cgggacgaaa agttttacct ccgcgacaac ttcgcagatg gccatgactt
     1681 tggcgcggat cgaaggcgcc gatcccaaaa cccgtgggtt gagtcttttt tatctcgagc
     1741 ttcgcaatga gaaaaatgaa cttgataaaa tcatggtgca tcgcttgaaa gataaattag
     1801 gcaccaaagc gctgcctact gcggagctca gccttcaagg gactccagcc agactggtgg
     1861 ggggcgaggg ggatggagta aaaaaaatct ccagcctttt caatatcacc cgcatttata
     1921 attccatttg ctccatctct catatgcgcc gagggttggc gttggcaaag gattattctc
     1981 tcaagcgcgc ggcctttggc aaacagctga ttgatcatcc actgcatgtt gaaactttga
     2041 tggatatgta tgtggattat gccgccagct ttcatttggt gatgagagcg gtagaaattc
     2101 ttggcaaaga ggagtgtggc aaagccatgc ccgaagaaag tgccgtttta agactcttga
     2161 cccccgtgat taaacttttc tcgggtaaaa aagccctgtc ggtaacgagt gaggtggtgg
     2221 aagtttttgg tggctccggc tatgttgaag acacggggat tcctcgtctg ctccgcgatg
     2281 ctcaagtctt ttccatttgg gaaggcacaa ccaatgtttt gagccttgac gtcctgcgtg
     2341 ccattgaaaa agaaaatgcc tttaagccat ttatgcaaga aatccaagga tgcttgctcg
     2401 cgatgatgaa agagcccatg gcaggccagc aactcaaagg agaaatccag gccatcgaaa
     2461 aagcgcttaa ggatttagaa agctatt
//
LOCUS       DATBTE010000158        14342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_634880, whole genome shotgun sequence.
ACCESSION   DATBTE010000158 DATBTE010000000
VERSION     DATBTE010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 14342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14342
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634880"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VIG33_05925"
     CDS             <1..189
                     /locus_tag="VIG33_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270408.1"
                     /translation="IGIWRDSQNKTGPDTGVSYSITTMVSVKWRARRLRVGYPPPEAV
                     QSKQIYLSRETFVEAPTT"
     gene            415..1449
                     /locus_tag="VIG33_05930"
     CDS             415..1449
                     /locus_tag="VIG33_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270409.1"
                     /translation="MISKDAVDDFFQLAAGTYKSAPWKYCSDSDVFGVVIPETKELHF
                     VSVMGGGGQCFGLATYRGIEGLDFFLSVIGGSGDIDPFTARRKQNGLLMEFTSKKYLD
                     DFNLEMAEQVHFKPASSKSWVMVKDMEPGWFPWAPKEKDILALATIMSLLPTFTKKQK
                     DNPEWTMGNGDDLFPVFIWKEKANDWKIEWWDQDKILKASNETVTETSVKPVDELKLQ
                     KAKSHKKSKQAIWEAYSFYMPEPVLDGDRPYFPEICVIMDHKANQCLEMEMIPPTQDR
                     AMVLRDLAIKTINKTSEVPQTIIIEEMDLLLGMITLKEVLEIEIKFDSTECGYNFRES
                     LYKKMDSFPR"
     gene            1640..2011
                     /locus_tag="VIG33_05935"
     CDS             1640..2011
                     /locus_tag="VIG33_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270410.1"
                     /translation="MKKIIYVLVLILISSLPLITSAKSSVVPFKSAHCEKVTNQLFHT
                     NSHRTVNFVLLQNNSVGVAYSIKMSGYDFAQKAFMDQMSNGVYRLRATHYDFTFDVEA
                     DAASAVSADSHDFFDLSCQYR"
     gene            complement(2241..2522)
                     /locus_tag="VIG33_05940"
     CDS             complement(2241..2522)
                     /locus_tag="VIG33_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8270411.1"
                     /translation="MGTRKVSKLSAFLKDKRLLAGLSQGEVAKKLGYTSPQFVSNWER
                     GLSDPPIRTLRKISTLYDVPLEEMHRVTLDATIQKVTEEIRKKFDGWKK"
     gene            complement(2582..3025)
                     /locus_tag="VIG33_05945"
     CDS             complement(2582..3025)
                     /locus_tag="VIG33_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270412.1"
                     /translation="MDKKPTGRLEVQNVTPQISLEELTTDLSAFLHFLSENYDGDKGT
                     DLPSFWMLIPKSIDVEYRYGTIDFFIEKEIFSRKILIVAEPLPDYLSDQERREKKAEL
                     LSLFQNAFTGDQTQQLLLLLSRLRPEARKTIVENFKRFKDEHAGK"
     gene            3126..3545
                     /locus_tag="VIG33_05950"
     CDS             3126..3545
                     /locus_tag="VIG33_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270413.1"
                     /translation="MDIIAKRNSSLGNMASNDEENYLQKIFYVVFRKYLPNWSDVVAF
                     SDRTGVNINTLRDIYYKDGLAGISTMDRVLKELLNLTPDKVAALISKIDQIEPISESS
                     SIWNSINAPEERKLYYALVAKALFEIDSKLEKASKKK"
     gene            3712..4116
                     /locus_tag="VIG33_05955"
     CDS             3712..4116
                     /locus_tag="VIG33_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8270414.1"
                     /translation="MSETTGLLHHVNIKVSDLKKSTEFWGWFLKMLGWELYDSWPKGV
                     SYKLGPTYLDFVQAEEQYANIPHQRDLVGLNHFAFHGRSRSHIDEITNTLRERGVNIL
                     YQDQHPFAGGPSYYAVYFEDPNKFKVEVVAPD"
     gene            4339..5109
                     /locus_tag="VIG33_05960"
     CDS             4339..5109
                     /locus_tag="VIG33_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270415.1"
                     /translation="MLDGSKLQLPQQFEWDFNVLSGDETTSLMKKVWPRYLIEESDLQ
                     EAPPSTELTDAELFRRFPMWGIRSKTDQKLVAFASAVLIPMDLTKKTLPDEGWQSAIQ
                     AYFSKFEANCLCLLSANVDPDFRKLRFSYFLLERAKELARSFGFNDVIAPVRPSQKDQ
                     FPSMPMIEYLEKQSDAGEIFDPWLRAHKNLGGEILNVCSQSVVIKATLSKWREWTGEA
                     LHQSGELLLPKGLVPLKVDVDANIGIYCEPNVWVHYRL"
     gene            5424..6017
                     /locus_tag="VIG33_05965"
     CDS             5424..6017
                     /locus_tag="VIG33_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY8270416.1"
                     /translation="MIDQKSELQEALKFIENIDALKNTYRKCLIMSGQREESTAEHSF
                     SLAMAVLCLVRFSNQKIDVTKTVKMALFHDLAEALLGDTFHYDKNTSPQAISEGEALK
                     QVLSPIARTELSTEIYDLWNEFENGNGPEAVFLRGLDRFLPILHNYKTKGHSWVKYGI
                     TKEKALQKNFHIEQSSDVIWSFTKNILDEAQSKGWIL"
     gene            6471..6959
                     /locus_tag="VIG33_05970"
     CDS             6471..6959
                     /locus_tag="VIG33_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270417.1"
                     /translation="MLHVLLALFLAPLFSHANVFDFKNLREIKTQGTLNAKCADFSGN
                     WSGTCIMDSKKRDKSVNISQFECTAWIISDSPYFIYLNGEKVTTTTNLSAVGDPWINK
                     ETVSTRLNDDQVEITFIDYDPRSSKPDFTSNGQFTLKNSRLFIKWKNSMGQSEDCELE
                     RR"
     gene            7449..7745
                     /locus_tag="VIG33_05975"
     CDS             7449..7745
                     /locus_tag="VIG33_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270418.1"
                     /translation="MKLLTIFVSLVISTSALASTIYYEGSEKTVTKTSLAPMERTLYV
                     FTDSGKCKFNTESLAMLHLDVLALTKAISQDNVAIRAQKNTDGSCDLILVEFVK"
     gene            complement(7916..8365)
                     /locus_tag="VIG33_05980"
     CDS             complement(7916..8365)
                     /locus_tag="VIG33_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270419.1"
                     /translation="MNTEIQNRLEKLAYKRTQPFCYSCYKETHSGTCESCRSDDLMRL
                     MVNVGCDYGVDWAIKYILESELTPVNLSRAFEDSVRECYPKEVTVGWMTLDSISVMKA
                     MDPIFWQCAQSEWEEQEAREGNIISFDSGLNYYYQTDIENFLDQEEH"
     gene            complement(8631..9047)
                     /locus_tag="VIG33_05985"
     CDS             complement(8631..9047)
                     /locus_tag="VIG33_05985"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEY8270420.1"
                     /translation="MAKSESNIQLWFKFSRVDLNMARTLFEGGQEEMWRGVTFHCQQA
                     AEKAIKGYLVYKKIPLSYIHDIGKLATQVLVHHPELDPLMKRASFLTKYAILYRYPDA
                     DVEAVTLDQVKEAIACAKTVIDQMAALIPFESQWEI"
     gene            complement(9052..9372)
                     /locus_tag="VIG33_05990"
     CDS             complement(9052..9372)
                     /locus_tag="VIG33_05990"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY8270421.1"
                     /translation="MHIELEKDKNLADIVSKLKAHFKPEKIFLFGSRAKGTATAESDY
                     DLFLIVKNSSKTKMERMDEANLVLWGRTSPVDVFVYTQKEFDESKSLFQSIPYIVNTE
                     GVEL"
     gene            complement(9472..10164)
                     /locus_tag="VIG33_05995"
     CDS             complement(9472..10164)
                     /locus_tag="VIG33_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY8270422.1"
                     /translation="MIKKKVCLYARVSTQDQASGLESQVRALNDYCRMNNITHYEIFA
                     DEGISGTKSSRPALDRMMKVVRDNEAECVVVYSFSRFARSTTHLLQGLEEMKKTNTGF
                     VSLTEKIDTNSPIGKAVFVIISAIAALERDLIAERVKNGLANARAKGKLIGRKKLRDS
                     DLIRKLLKAGMTYRAISTVANCSHGSVHQEKLAMKKDEALALKMKLEQEELEKAQMTP
                     TVQFPEQSTEAA"
     gene            complement(10281..10598)
                     /locus_tag="VIG33_06000"
     CDS             complement(10281..10598)
                     /locus_tag="VIG33_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270423.1"
                     /translation="MTAALNIQKVQRAPSIEMKAVLEILNADSFLMESVMPFIDFQNE
                     NIYWDQIFKNSFGSGHRGALLFAYAIWTDEVRSNPFDAAFSMGPKLQIAVLKAMALRW
                     GLQ"
     gene            complement(10595..10810)
                     /locus_tag="VIG33_06005"
     CDS             complement(10595..10810)
                     /locus_tag="VIG33_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270424.1"
                     /translation="MNFDKIIPLIASIVIAAATVGKLDVLTLWIYKAQAKLVYESRAS
                     AWGSPRFFPEHPNTRKRNNKLTKGGDL"
     gene            complement(10797..11144)
                     /locus_tag="VIG33_06010"
     CDS             complement(10797..11144)
                     /locus_tag="VIG33_06010"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY8270425.1"
                     /translation="MRSSLVRAGITLREVRCQGTHCRKITSGKQSAKPYYGFCYFEGR
                     IVKDPREFPTLAIIHQRWGRGLSAHSIMKELDQKKIRSREGKQWSWAAVNNILKRFET
                     KKIILKGKSYEFR"
     gene            complement(11412..11660)
                     /locus_tag="VIG33_06015"
     CDS             complement(11412..11660)
                     /locus_tag="VIG33_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270426.1"
                     /translation="MNQPIDRQNFRFAVTSEVSAELSIKFGLVSDECKIRPKEGALCE
                     AGFAAKNPPKDTGMDNYKICVKTTSEKNHPPTDSSSTR"
     gene            11985..12431
                     /locus_tag="VIG33_06020"
     CDS             11985..12431
                     /locus_tag="VIG33_06020"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8270427.1"
                     /translation="MENKIDVRIRPAEKDDLVRLADLSLQLGYENSVNNLERRLEALN
                     SSGDHVLLVAESSSGSVIGLAYFKKHKSLFVEESLEVGGIVVDKDCRGLGVGKLLMSK
                     AEDVAKNWNVRTIRLTSNIKRADAHSFYRSLGYEHPKTSHFFIKAL"
     gene            complement(12860..13402)
                     /locus_tag="VIG33_06025"
     CDS             complement(12860..13402)
                     /locus_tag="VIG33_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270428.1"
                     /translation="MGSRLTIIYFLLASSISLAKPFPSSGDNINCSLSVYDITIFAPC
                     SYGPSCQIDKAQASVVFETLEGALDKHLNGSVSVKEIRGFREANGGPISKETQILIKL
                     IQDAELRLEIDYNNPLFSANLWSESDSQNFKLSLKKRSDMSRGNRNNGPISELVKLKS
                     NDSTEGGVYGQLSCSPSRVK"
     gene            complement(13802..14071)
                     /locus_tag="VIG33_06030"
     CDS             complement(13802..14071)
                     /locus_tag="VIG33_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270429.1"
                     /translation="MRSSAQLMTKAALPILQFERKELQDCKVDRWGNNICNPLLPKYS
                     NPIWILADDYIPQVQWVNIETNIELPDKADFEAYRKCVESEISTL"
BASE COUNT         4089 a         3047 c         3058 g         4148 t
ORIGIN      
        1 atcggaatat ggcgggattc tcagaataaa acaggaccag atactggtgt cagttattca
       61 atcacgacaa tggtctcagt taagtggcga gcacgaagac tacgcgttgg atatccaccg
      121 ccggaggcgg ttcagtccaa acaaatctat cttagccgtg agacgttcgt ggaagcgccg
      181 acaacctaat cgagcacaat acccctatgt tacagaagcc acaccattaa atcagcgtgg
      241 ttttcttgaa gaggtggggt ttttaagcca agctgccaat ggggaactgg ccgccactcg
      301 aggcatgtag gcaagattcc agaattagcc cggagatacg attttattgg acttgggcaa
      361 ctccgtcttg ctaagaccct cagaatggaa aatatacaag ctccgaagcc acctatgatt
      421 tcaaaagacg cagtcgatga cttttttcag ctcgccgctg ggacctacaa atcagcaccc
      481 tggaagtatt gttctgactc tgatgtcttc ggagttgtga tcccggaaac aaaagaactt
      541 cactttgttt ctgtcatggg cggtgggggc cagtgctttg gactcgccac ttatcgtgga
      601 atcgaagggc ttgatttctt tctctccgtt ataggtggct ccggtgatat cgatcccttt
      661 actgcccggc gaaaacaaaa tggactcctc atggagttca cttcaaaaaa atatttagac
      721 gactttaatc ttgagatggc agagcaagtg cattttaaac ccgccagttc aaagtcatgg
      781 gttatggtaa aagatatgga acctggttgg tttccttggg cacctaaaga aaaagacatt
      841 ctggctctgg ctacgatcat gtcccttttg ccgactttta caaaaaagca aaaggataat
      901 ccagaatgga ccatgggtaa tggtgacgat cttttcccag tctttatctg gaaagagaag
      961 gccaacgact ggaaaatcga atggtgggat caggataaaa tcttaaaagc cagcaatgag
     1021 actgtaacag agacttcagt caaaccagtg gatgagctga aactacaaaa ggccaaaagc
     1081 cacaagaaat ccaaacaagc tatttgggag gcttactcct tttatatgcc ggagccggtg
     1141 ctggatgggg atcgtcccta tttcccagag atttgtgtga tcatggatca taaagcaaac
     1201 cagtgtctgg aaatggaaat gattccgcca actcaagacc gagctatggt tcttcgtgac
     1261 cttgcaatca aaactatcaa taaaacaagc gaggtgcctc aaaccattat cattgaagaa
     1321 atggatctcc ttttgggaat gatcacttta aaagaagttt tggaaatcga gattaagttt
     1381 gactcaaccg agtgcggtta taacttccga gaaagcctct ataagaagat ggactcattc
     1441 ccacgctgaa gccccccccg gcctgtggtc aatcttatga cgagaagcct tctatagagg
     1501 acccaaccaa cgaaaattaa gtcgatatga ttatcttttt acatgcctgg aattaaattg
     1561 cacaagtgca aatgttaaat taaaagcacc ctattattta acaaacgatg gtgtgtccac
     1621 caaacaatag ggagtctttg tgaaaaaaat aatttatgtc cttgtgttga ttttaatctc
     1681 aagcctaccg ctcattacat cagccaaatc ctccgtagtc ccattcaaga gcgcacattg
     1741 tgagaaggtt accaatcagc tctttcatac caactctcat cgaactgtta actttgtatt
     1801 attgcaaaat aactcagtgg gcgttgcata ctctataaaa atgtctggtt atgattttgc
     1861 acaaaaagcc tttatggatc agatgagcaa tggcgtatat cgccttagag ctacacacta
     1921 tgactttacg tttgatgttg aggcagacgc cgcatcagca gtttctgctg attcccatga
     1981 tttttttgat ctgtcttgtc agtatcgtta agcaatatga tcttgctgtt cgaacattga
     2041 taatttaatg aataagctgg aggcaaaaat ctccggcttt tttttatatg ccttatgacc
     2101 aaggccccca acaacgccga aggtccttgt tactctattg atgcggtcgc caaacccttc
     2161 gaatcacaat tctaaagcag gttttactac tcatgaccag gtagctcaaa ccgctaccaa
     2221 aattcccgct ggaacttcac ttattttttc cacccatcga acttttttct tatttcttcg
     2281 gttacttttt ggatagttgc atccagtgtc actcggtgca tttcctcaag ggggacgtcg
     2341 tatagagtcg agatctttcg aagggtccga attggaggat cagaaaggcc acgctcccaa
     2401 ttcgaaacga attgcggaga cgtatagcca agtttttttg cgacctctcc ttgggaaagc
     2461 cctgcgagca aacgtttgtc ttttaaaaag gctgaaagtt tacttacttt tctggtgccc
     2521 accacatact cctaaaataa ccttggcggt caaggtgcgc caatagaaac acttctaagt
     2581 tctacttgcc cgcatgctca tctttaaatc gcttgaaatt ttctacaata gtttttctgg
     2641 cctctggtcg aagacgagaa agcagcagaa gaagctgctg agtctggtcg cccgtaaagg
     2701 cgttttgaaa tagcgataac aactcggctt ttttctcccg acgctcttga tcggaaagat
     2761 agtcaggcaa tggttcagca acgataagaa tcttgcggct aaaaatttcc ttttcgataa
     2821 agaaatctat agtaccgtac cggtattcaa catcaatact cttaggaatc agcatccaga
     2881 agcttggcaa gtctgttcct ttgtctccat catagttctc acttaaaaaa tgaaggaacg
     2941 cgcttaggtc agtggtcagc tcctcgagag aaatctgagg agtcacattc tgaacctcaa
     3001 gccttccagt tggtttttta tccaagccag cctccttttt acttccgatt aatctattat
     3061 gtatatcaca ttaaaacaag tattatttat gtgtattaca caaaacgatg gctctttgtg
     3121 cttaaatgga tataatagcc aaaagaaact caagtttggg gaacatggcc agcaacgacg
     3181 aagaaaatta ccttcagaag atcttttatg tggttttccg caagtacctg cctaattggt
     3241 cagatgtggt cgcattttct gatcgaacag gtgtaaatat aaataccctg cgggacattt
     3301 attataaaga tggcttggcc gggatcagca cgatggaccg agtactcaag gaactgttaa
     3361 atttgactcc tgacaaggtt gccgccttaa tctctaaaat cgaccagatt gaacctattt
     3421 cagaatcatc aagcatttgg aactcaatta atgctcctga agagaggaag ctttactacg
     3481 cgcttgttgc caaggcgtta ttcgaaattg attcgaagct cgaaaaagcc tcgaagaaaa
     3541 aatagaacca aggcgatagc aagacgagac tcgttttata tcgctcgtct tttgaaccaa
     3601 gattagctga attgaatgca taaaaaagaa agggttttta agctattaag ggctctttcg
     3661 gtagactttc aaatctcctt actataggta tttctcaaaa gggagactgc catgagtgaa
     3721 accaccggac tcctgcatca tgtaaatata aaagtttctg accttaagaa atctacagag
     3781 ttttggggat ggttcttaaa aatgctgggg tgggagcttt atgattcgtg gcctaaaggc
     3841 gtgagttaca agttgggacc gacctacctg gattttgtcc aagcagagga acagtatgca
     3901 aatatcccac atcaacgaga tttggtagga ctgaatcatt ttgcctttca tggacgctcc
     3961 agaagtcata tagacgaaat tacaaatact ttgagggaac gtggcgttaa cattctttac
     4021 caggaccaac acccgtttgc cggagggcct agctactacg cggtttattt tgaagacccc
     4081 aataaattta aagttgaggt tgtggccccg gattagaaat ccggtcagaa attcctccag
     4141 agaggttttt aaggcgcgat ttgctactct cctgctcaag gctattacca gccggtcaag
     4201 agcgcctgtc tttacgtcag ggccatttta ttgaatctga tgactgcgta aaactgaagg
     4261 cccagcaact cgatatgatt cctcaagtga ccatccaaag gaggtgacag acagcctatc
     4321 ataaaatacg ctggtgaaat gctagacgga tctaaattgc aattgccaca acagttcgag
     4381 tgggacttca atgttttatc gggagatgag acgacttcgt tgatgaaaaa ggtttggcca
     4441 cgatatttga ttgaggaaag cgaccttcaa gaagccccac catcaaccga gttgactgat
     4501 gctgaactct tccgacgatt tccgatgtgg ggcattcgaa gcaagactga tcagaagctt
     4561 gttgcgtttg ctagcgcggt tctcattcca atggatttaa ctaagaaaac tttgccagat
     4621 gagggatggc aatcggccat ccaagcatat ttttcgaagt tcgaggcgaa ctgcttatgc
     4681 ttactgtccg ccaatgtcga tccagacttt cgtaagctca gattttccta ttttttatta
     4741 gagcgggcaa aggaactcgc tagaagtttc ggatttaatg acgtgatcgc accagtaaga
     4801 ccctctcaaa aggatcaatt tccaagcatg ccaatgattg aataccttga aaaacagtca
     4861 gatgcaggtg agatttttga cccttggctg cgggctcaca agaatctcgg gggagaaata
     4921 ctcaacgttt gcagccaatc tgttgtcatc aaggcaacgc tatcaaagtg gcgcgagtgg
     4981 acaggggagg cactacatca atctggcgaa ctccttcttc ctaagggact tgttccattg
     5041 aaggtcgatg tcgatgcgaa tataggaatc tattgtgaac caaatgtttg ggtccactac
     5101 aggttgtgag gaaaaccgtt cacctaaatc acttgataag gagaaaagtc acgctctgta
     5161 aatcacaggc gaagaagtta atctgaggag ctattttttg taaagaaagg agtgagtttc
     5221 aattcactca tttctggcca aagagtggag atcgttaaag cacactcaga gcatcgccac
     5281 cgatcttttc gatccaactg cggagaagac caagtcagcg atcgcgcatt tgttttcttt
     5341 acaatcttcg tatcgaaggg tatttctacg ccatctagcg aatggaataa taatatgaga
     5401 ttgaattttt aaaggagggt ccaatgattg atcaaaaatc ggaacttcaa gaagccttaa
     5461 aattcattga aaatattgat gccctcaaaa atacttatcg taagtgtttg atcatgagtg
     5521 ggcaaagaga agagagcacc gctgaacatt cattttcttt agcaatggcg gtcctttgcc
     5581 tggtacggtt tagtaatcag aaaatcgatg tgactaaaac ggtaaagatg gcattgtttc
     5641 acgatcttgc ggaagcctta ttgggcgata cattccatta cgacaaaaat acgagtcctc
     5701 aagcaatttc tgaaggagag gctcttaagc aagtcctgag tccaattgct cgtaccgagt
     5761 tatctacaga aatttatgat ttgtggaatg aatttgagaa tggcaatgga cctgaagctg
     5821 tatttcttcg tggtttagat cgctttctcc cgattctgca taattataaa actaaaggtc
     5881 attcctgggt taaatacggt atcaccaaag aaaaggctct tcaaaagaat ttccatattg
     5941 aacaaagttc tgatgtaata tggagcttta caaaaaatat actggatgaa gctcaatcaa
     6001 aaggatggat tctctaaatg atgttttttt cagatccatt ctttgaccgc cattaagccc
     6061 ttcaatcttg gttgaggaac tactaagaat tcactgatta gccagcggcc tgaaaaaccc
     6121 tggctatttt tatttgtaat tctgaccagg agccatgggc ttagcaggtc cggctcgtgc
     6181 tactcctctg gtcatgaaac cccaactccg gcattttaag gcacaaactg ctactgtccc
     6241 gagtatccat aataggctct ggtcatttga aggcgtcttt tggggcaatg tcattttagg
     6301 gctataaagc cagttcggag ctgatggccc ggctaatcgg aaagatttgg tttcaattca
     6361 ctgaaaataa aggacgggtt accccaacaa tgttgcagcg atggttaact gtgtttgcaa
     6421 ttgactctta atgcctacgc tgcaatcctc caataaaaag ggagattcac atgcttcacg
     6481 ttttattggc attgtttctt gcgccacttt tttctcatgc aaacgttttt gattttaaaa
     6541 atctaagaga gatcaaaact caaggcactc tgaatgctaa atgtgctgat ttttcgggga
     6601 attggtcggg cacttgcata atggatagca agaagcgaga taaaagcgta aatatctctc
     6661 agtttgagtg cacagcttgg attatctccg attcgcctta ttttatatat ctgaatggtg
     6721 aaaaggttac cactaccact aatctgtcag ctgttggtga tccatggatt aataaagaga
     6781 ctgtgagtac tcgtttgaac gatgaccaag tggagatcac ttttatagat tatgatccca
     6841 gatcatcaaa accggatttt acttctaatg gacaatttac cttaaaaaat tctcgcctct
     6901 ttataaagtg gaaaaactcc atgggacaat ctgaggattg cgaactagaa agaagatgag
     6961 attcagttga ttcaacaacc atcgcctaaa ggcgatggaa tgcttaacgg cggactggcc
     7021 gccgtctaaa attacgcggc taaagcggct ttggaaaata aaaattctag caacaatagt
     7081 gagcggacaa tgtcacttgc taatagaaaa aatcccgcgc cctaagatgc agatgagcta
     7141 agccagactg agggctcgct ttttgtaatg aaatgagcga gtttcaattt atgcattttg
     7201 gatcagcgga tgacgatcat taaagccact atcctggcca gcgcctgggg aagtcagaag
     7261 aacagcttca aagacgccct cccgcgaaac aaatcttaac tttattcccc atcagatccc
     7321 attacccgcc cgctcagtac cgtcagggtc ccataatgaa aagcgccaag attcattgag
     7381 atttactcaa aaccattgac catttgaggt tcgctgaagt aatcccaatg caactccgga
     7441 ggaacattat gaaattatta actattttcg taagcttggt tatttcaact tcagcacttg
     7501 cttcaacaat ttattatgag ggttcagaga agactgttac aaaaacttct ttagctccaa
     7561 tggagcgaac actttacgtt tttactgatt ctggaaagtg taaattcaat accgaatctc
     7621 ttgctatgtt gcatttagat gtactcgctt taacaaaagc tatttctcag gataatgtcg
     7681 ctattagagc tcagaaaaat actgacggtt cctgcgattt aatacttgtt gaatttgtaa
     7741 agtaaaaccc taatatctta ggcactaagc tttcagcaat accacgagat aagtcggaaa
     7801 cgaaaatttc cggcttgttt cacgtacctt tttagtcatg agacaattgc cgctagaagg
     7861 gtgctgctac cggagtgact aagaactccg gcgccggaag taattgacca aggtgtcagt
     7921 gttcttcttg gtccaggaag ttttcgatgt cagtttgata atagtaattc aaaccgctat
     7981 caaaggaaat aatgtttccc tcacgggctt cttgctcctc ccactcagac tgggcacatt
     8041 gccaaaaaat tgggtccatg gccttcatga ctgaaatcga atccagagtc atccagccca
     8101 cagtcacctc tttaggatag cactcacgaa ctgagtcctc aaaggcccgg ctcaaattga
     8161 ccggagtaag ctcggattcc agaatatatt tgatcgccca gtcgacccca tagtcacagc
     8221 cgacattgac cattaagcgc atgagatcgt ctgacctgca agattcgcaa gttccgctat
     8281 gagtttcctt ataacaggaa taacagaatg gttgagtgcg tttgtaggca agcttttcaa
     8341 gtctattttg aatttcagta ttcatgattt ctttcctttg cttcttgttt tattttccct
     8401 ttcttagggt tccttcacga agcttgagga acccgaaaag ggaaaaagag agcgcaggca
     8461 aagacctcgg accgaccgcg tatgcggcag ggcgaatgac atttctataa ggaatggcgt
     8521 gaagcctgcg gagggacgaa aacaggtctt tgccggagag aacgtcacga aaagaagcaa
     8581 gggagtcttg ttatttgaaa tttaatgttt gaaaagccaa aaatgccggc ctagatttcc
     8641 cactgacttt caaagggaat aagcgctgcc atttgatcaa tcacggtctt tgcgcaggca
     8701 atagcttctt tgacttgatc caaagtgacg gcctcgacat ccgcgtcagg gtatctgtag
     8761 agaatggcgt attttgtaag gaaagacgct cgcttcataa gcgggtccaa ttctggatga
     8821 tgcaccaaaa cctgagtggc tagttttcca atgtcatgaa tatatgaaag cgggattttc
     8881 ttatagacca aatagccctt gatagctttt tcagccgcct gttgacagtg aaaagtaact
     8941 ccccgccaca tttcttcctg cccaccctca aaaagagttc gcgccatgtt cagatctaca
     9001 cgggaaaatt taaaccaaag ctgaatattg ctttctgatt ttgccaaacg attaaagctc
     9061 cacaccctca gtatttacta tgtagggaat ggactggaac aaagatttac tttcatcaaa
     9121 ttctttttgc gtataaacaa agacatctac aggtgaagtg cggccccaca aaaccaaatt
     9181 ggcttcgtcc atgcgctcca tcttcgtttt gctcgagttt ttaacgatga gaaaaagatc
     9241 gtaatcactt tctgccgtcg cagtcccttt tgcgcgagag ccaaaaagaa atatcttctc
     9301 gggcttaaag tgagctttaa gcttcgatac gatgtccgcg aggtttttgt ctttttctaa
     9361 ttctatatgc atacaaactt tccgttaaag ttagtatgat ttagaaaaag gtcttatttc
     9421 ttagttaaaa atttcctgga atagaaaatt cttaattaaa aaatcgaggc cttaagccgc
     9481 ctctgtggat tgctcgggga attgcaccgt tggagtcatt tgagcttttt caagctcttc
     9541 ttgttccaac ttcattttaa gagccaaagc ttcatctttt ttcatcgcaa gcttttcttg
     9601 gtgaacactt ccatgagaac aatttgccac tgtagaaatc gctcgatagg tcatgcccgc
     9661 ttttaaaagt ttacgaatca aatccgaatc tctgagcttc tttctcccga tcaatttgcc
     9721 tttggctctc gcgtttgcca gtccgttctt tactcgctca gcgatcaggt cacgctcaag
     9781 ggccgcaatc gccgagataa tcacaaacac cgcttttcca atcggagagt tcgtgtcgat
     9841 cttctcggtt aaggaaacaa agcccgtatt ggtcttcttc atttcctcaa ggccctgaag
     9901 taaatgcgtc gtgcttctcg caaatctcga gaaagagtag actacaacac attccgcttc
     9961 gttgtctctg acaactttca tcatccgatc cagtgccggg cgggatgatt tcgtcccgct
    10021 gattccctca tcggcaaaga tctcgtaatg agtgatattg ttcatgcgac aatagtcatt
    10081 caaagctctg acctgggatt ccaggccgga ggcctgatcc tgagtgctca cgcgagcata
    10141 aaggcatacc tttttcttaa tcattagctt cccctttctt atagtttttt ttgactagat
    10201 tttcaatttt attggtattt ctctgcatca tagagtcacc tccttggttg gttgttggct
    10261 atattggttt atagacgaat tcattgaagc ccccagcgta acgccatggc tttgagcacg
    10321 gcgatctgaa gctttgggcc catgctaaag gcggcgtcga acgggtttga ccggacttca
    10381 tcggtccaaa tggcataggc aaatagcaaa gccccgcgat gacctgagcc aaaagagttc
    10441 ttaaaaatct gatcccagta aatattttca ttttgaaaat caataaatgg cattactgat
    10501 tccataagaa atgaatcggc gtttaaaatc tcaagcaccg ccttcatttc tatggatggg
    10561 gctcgttgta ctttctgaat gttgagtgct gctgtcataa atcacctcct tttgttagtt
    10621 tgttatttct tttacgtgtg tttggatgtt cgggaaaaaa tcgagggctt ccccaggccg
    10681 aggcgcggga ttcatacaca agcttggcct gggccttgta gatccataag gtcagaacgt
    10741 cgagcttgcc gacggtcgct gccgcgatca caatggatgc aatgagcgga atgattttat
    10801 cgaaattcat aagatttccc ctttaagata attttttttg tttcgaatcg tttaagaatg
    10861 ttattcaccg cagcccagct ccattgcttg ccttcgcgag aacggatttt cttttgatcg
    10921 agttctttca taattgaatg tgccgaaagc ccccgccccc aacgctgatg gatgatggct
    10981 aatgtcggaa actctctggg gtctttgaca attcgcccct cgaaatagca gaagccgtaa
    11041 taaggctttg ctgactgctt gcctgaagtg atttttcggc aatgagtgcc ttggcaccga
    11101 acctctctca aagtgattcc cgcgcgaacg agagatgagc gcacgccatt tttagaacag
    11161 ccatactcat tggctatttc cgcaagcgag agaccttttt ggtacttttc aataaattct
    11221 tggcttaaat cggattttac ttcggttaaa aatgttgaga aatcaagagg atagatccgt
    11281 tcggatattg gaatgcatgg ttcaccacta aattctctat atatatcaaa tacttacggc
    11341 catgagtaaa aataaaattt gatagagaat atcccttgaa catcaagccc tagggaaata
    11401 tggacagcac tttatcttgt actgctagaa tcggttggcg gatgattttt ttcagaagtt
    11461 gtttttacgc agatcttata attatccatg cctgtatctt tgggtggatt ttttgcggca
    11521 aatccggcct cgcaaagagc accctcttta ggacgaattt tacactcgtc cgatacgagg
    11581 ccgaatttta tcgaaagctc tgcagaaact tccgaggtca cagcgaaacg gaagttctgg
    11641 cgatctatgg gttgattcat ggcttggtta ccggaccgtt cctgacggat caggcgatcg
    11701 aattagcgac gacctggcta acgcgcttaa ccgagaatcc aaagcagtag ctcgatttcg
    11761 ctgaacttgc cacagggaca aggttcctct ttgcttaacc taaagagaat ctctgcaagc
    11821 tgtcgtcaga tcgatttacg caggcatact gggggcattg ggctctccga tcacttaagc
    11881 ccgctatatt gaatttaagg gctccgggat tgaagcaatc taagggattc atccaataga
    11941 tttctttgat gactggctta ctgttttcga tttactaatt gcatatggaa aataaaatcg
    12001 acgtgcgaat tagaccggct gaaaaagatg atttagttcg tttggctgat ctctcattac
    12061 agcttggata cgaaaactcg gtgaataatc tagagagacg attagaagca ttaaatagtt
    12121 ctggagatca tgttctatta gttgcggaat cgtcttcagg cagtgttata ggcctagcat
    12181 attttaaaaa gcacaaatcc ttgttcgtcg aggagagcct cgaggttggt ggtatcgttg
    12241 ttgataagga ctgtaggggt cttggcgttg gaaaattact catgtcgaag gcggaagatg
    12301 tagctaaaaa ttggaatgtt cgaacaatta gattaacttc aaacattaaa agggctgatg
    12361 ctcatagctt ctatcgcagt ctcggttacg aacacccaaa gacatctcac ttttttatta
    12421 aagcgcttta ggaaatttaa cctatcaata atttttagga ccagaattaa aacattctgg
    12481 tcttttgtta tcggtgattt tttcgtctgc attaaaatct atatctaatt gagcccgctg
    12541 atttcaatcg aatccttctt tggtaatttc caacagtatc aattctcttt ttaaactcga
    12601 gataagggtg ctattaggta ccaagctttc agtaatacta cgaaataagt cggaagcgaa
    12661 aatttccggg cttatttcac gcacctttct ggttatacca ataaaaaagc gtaccgatct
    12721 tcttcctgtc atcaaagcaa aaggtaaaga tattctgtgc taataattta gcataaaaaa
    12781 tgcactattt aatagggcat tagaagaaac caaagctttt gtcttttcca aggctgcagc
    12841 tgttccagat ttcaatttgt tacttaactc gactggggga acagctcagc tgaccgtaaa
    12901 ctcccccttc cgttgaatca ttcgatttca acttgaccag ctctgaaatg gggccgttat
    12961 ttcggttgcc ccttgacata tcactccttt tcttaaggga tagtttgaaa ttttgagaat
    13021 cactttccga ccatagattc gctgaaaata atgggttgtt atagtcgatt tcaagtctta
    13081 gttcggcgtc ctggattagt ttaatcagta tttgggtctc ctttgaaata ggccccccat
    13141 tggcctctct aaatccacga atctctttta cagaaacgga accatttaga tgtttatcaa
    13201 gagcgccctc tagggtttca aatacaaccg aagcctgggc tttatcgatc tgacaactag
    13261 gaccataaga gcaaggagca aaaattgtaa tatcatagac actaagtgag cagttgatat
    13321 tgtctccaga ggaggggaat ggtttcgcca gagaaataga actagcgagt aaaaaataaa
    13381 ttatcgtaag gcgtgatccc ataaaaatcc tttcaattag tttcaattca tcagatgaat
    13441 tcctaatata aaaaaaaatt aaaggaaaac ttagttttgc aatttgttaa aatgaagttc
    13501 tcgccgccga attttgaagt ggcttcgact tgaatacatc gctctgtgtt gccgaagaaa
    13561 tcaatggcat ggacctcgtt cgtaacaaac ctggcttttc agcacagatt cccccggaaa
    13621 tttgcaatcg cgaatttcct ttccataagg tgattcgaga aagcttcaca tgggcggcgc
    13681 ccttgcaagt ttttccgtct tcgctttcac gacgcgggaa ttcgatgcca gtaaagaatc
    13741 catcctgatg gacaccggag tttgatggac attgttcaaa ggttgttaga gtttgccctc
    13801 tctaaagagt gctgatctcg ctttcaacgc attttcgata cgcttcaaaa tccgccttgt
    13861 ctggcaattc aatattagtt tctatattca cccattgcac ctgggggatg taatcatcag
    13921 ccaatatcca aatcggattt gagtacttag ggaggagggg gttgcagata ttattgcccc
    13981 atcgatccac tttacaatcc tgtaattctt tgcgctcaaa ttgaagtatc ggcaacgcag
    14041 cttttgtcat taattgagca gaagacctca ctgaatcccc ttcttgttgc tcatagcatt
    14101 ccttaaaatg atacttgagg gtggctgaat ctatcgaagt aagtttagct gttattgcca
    14161 aatcataatc ggaatgagct tcatatcgat aacgtctcct gtattaataa gtgcgttgga
    14221 taagcaacat taggcggcgt attttagact gtctttttga aaaaatgagc gaaccttttc
    14281 tggatttttt tgtagagatt tcatcgaccg agtcacctgc tttttaaagc tcttcttatc
    14341 gg
//
LOCUS       DATBTE010000159        13921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_88748, whole genome shotgun sequence.
ACCESSION   DATBTE010000159 DATBTE010000000
VERSION     DATBTE010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 13921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13921
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88748"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1147
                     /locus_tag="VIG33_06035"
     CDS             <1..1147
                     /locus_tag="VIG33_06035"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY8270430.1"
                     /translation="APASVTEKLIISKVGDDVSLLKPGTEVRTIGGRPWQESLQEAVR
                     FTSGSTDRMRRNQAAKWLLPFILGTKETFKDLPIEGILNGQVVKENISRVLTLSDGNA
                     EPPPAATGKGSINARIINGNIGYLRIDAFMGTKMDILLDQAMSRLLHTEGLLIDVRLN
                     GGGTPVGDRVLSYLIEQEIMRYSAKIRNSDMLMAMQPLYATGFDFINGNWSEVWQYKV
                     QPNAHGKYKKPVVVLTSAYCFSACDTFVSALKENNLAKVAGEATGGGTGSPVVFNLPY
                     SKFKFRYSVQKGYKAVSGDLLEGVGTGVDLDISPTAQERIAEKDEQLKTAVAYLKSLM
                     TDQPTLQFHLNSVAEENSEFERAADRQPLRSPLLEMESEILKSNFEN"
     gene            complement(1165..1860)
                     /locus_tag="VIG33_06040"
     CDS             complement(1165..1860)
                     /locus_tag="VIG33_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270431.1"
                     /translation="MKYMLPILFLIFLTQICFGQESSELEVDVERSIVDSDAAKIDAA
                     EAKKMAEDEKKRKEQLKIESRRTLKEAKGAEAQAEKEATLARKESAKVAAEAQGYEKQ
                     IKIAEAQQAKAKEKIDRARVRLQKANQHRDQILDKKRDTLRELEKLESQIKDQEHMVS
                     EANAEADLAQKEAIEAKRRLREAAIELKVIQREAKKKIKKAKLRTFKSQASTRKSETT
                     QRKIDQQISNTKR"
     gene            2095..2757
                     /locus_tag="VIG33_06045"
     CDS             2095..2757
                     /locus_tag="VIG33_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY8270432.1"
                     /translation="MSKDATETVGDHFQNDLLKFARDQSLAVILKASSYIKPGIRENE
                     AKALIQEIQTQMGAPKSWHPPQIRFGENTLLPFGQQGHENLSLKENDIYFFDIGPIFY
                     GHEGDVGRPFVVGNDSEMKKCCRDAASIWQEVRDHWVSTNATGKELYQVARFSAENRG
                     WLLSLHEANGHRISDFPHAARMRGSVEKFEQKPAPNRWILEIQIRHPKKSYGAFYEDL
                     LI"
     gene            complement(3034..3660)
                     /locus_tag="VIG33_06050"
     CDS             complement(3034..3660)
                     /locus_tag="VIG33_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270433.1"
                     /translation="MKLSKLSSRLVMAAMGILANHSALAAGGGNTGGNCYNMQIIAHK
                     SKTADMTGTNGRALFIALNTRTKILLQEGPFNIIDRNGTDGQASFSLPAPDPTNSGKT
                     AYSVYMRLVGKPHSGINMATCGTDALGETYCSGDQVNMSRIAGSSKFLNVSQQLLYIY
                     ADINADGIVERVPLFDGRLSEYFWQVDSMGRLHAQVRFCPVSTTVAAP"
     gene            4030..4872
                     /locus_tag="VIG33_06055"
     CDS             4030..4872
                     /locus_tag="VIG33_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEY8270434.1"
                     /translation="MNSNYIPRKLLFGNPDIRDLKISPDGRHIAYLAEHHGVLNIWAQ
                     DVGNESTARPLTHDTKRGIFSYDWTFQPGTLIFSQDIDGDENFGLYTLNVLNGETTEI
                     TPPGHFTTHVSELSHLRPDEVVILTNNRDPMYFDYKILDLRDKTQKDLFTNTENFALI
                     HFDKYFRPVIAIKSNADGSYTGSLWNPHTKSFFEKFVTAFEDSMSSTTISTSNDGRKV
                     YLLSSEGRDKATFQEWDLATDTLTVLAEDSKSDIQAIQIHPGSGKPLMATSYYVKARG
                     PLLG"
     gene            4841..5926
                     /locus_tag="VIG33_06060"
     CDS             4841..5926
                     /locus_tag="VIG33_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8270435.1"
                     /translation="MLKREVHFLDEEFQKHYKNIQNKLQTEFKITSLSLSLEHWVIMT
                     DEAHKPAAFYFFNTKTQTLSAPLHTRKSLLPHTAKLQPMECLEILSRDGFPLVSYLTR
                     AKEQAGKALILLVHGGPWSRDRYGFNVYHQWLADRGYNVLSVNFRSSVGFGKNFLNAG
                     DQQWGHKMHEDLLDAVNWAVNKGHADSSQVVIMGASYGGYAALAGLTFTPDTFAAAVD
                     IVGPSNLETLLSTIPPYWESFKANLYRRVGDPTTEAGQKLLKKCSPLTHVDKIKKPLL
                     ILQGANDPRVKKAEADQIFNSMLSKKIPVQYILFPDEGHGFAKSQNNMGANAIIEGFL
                     QKFLGGRLEDIEDDLKESSAQFITALQ"
     gene            complement(5952..6470)
                     /locus_tag="VIG33_06065"
     CDS             complement(5952..6470)
                     /locus_tag="VIG33_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEY8270436.1"
                     /translation="MKIWFLMVLALSIGFSSVSLAKSKKKNQIQNFRGFVQSCHDGDT
                     CRVLVNNKSLKIRFSGIDTPEIKQPEGPMAKGFTESLILHKIVDLKCEGMSFDRLACI
                     VFYESKNINEEIVRNGFAWDSPKHSHGEYNLLMMQAQNKEIGIWKTTRVSPYCFRHKN
                     SKKCVESKLYME"
     gene            6791..7117
                     /locus_tag="VIG33_06070"
     CDS             6791..7117
                     /locus_tag="VIG33_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270437.1"
                     /translation="MKTISWITTMLFAASAYAQAPMSVIDSPDINSSSQNEEAYQEYY
                     DQDYSDQNYFDQEQNNSEDKSSESNKEPEFTPTQRSGTPGYIGRRPGYPGGRLVRGPG
                     GHIGGP"
     gene            7554..8543
                     /locus_tag="VIG33_06075"
     CDS             7554..8543
                     /locus_tag="VIG33_06075"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY8270438.1"
                     /translation="MRTGQIFEVIGKTPLIKIKSLSEATGCEIYGKAEFLNPGGSVKD
                     RAALGIISEAEKLGQLKSGYTIFEGTAGNTGIGLATLAPQKGYRCVIVMPNNQSQEKY
                     DALKALGVELITVPPCPFTNENHFYHTAKRLATERPDSFWANQFENTANFKAHYQTTG
                     PEIWEQMNGNIDAFISAVGTGGTLSGVSRFLKDKDKSVHIRLVDPFGSGLYSYFKTGQ
                     LSSTGSSITEGIGIMRLTANFKEAVIDEAVQVNDQEMLSTLYHLAEHDGLLAGTSAAL
                     NVYASYKYALENKNSGKRIVTILGDSALRYQSKIFNPQFLSEKGLFKKTLLSS"
     gene            8772..10241
                     /locus_tag="VIG33_06080"
     CDS             8772..10241
                     /locus_tag="VIG33_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270439.1"
                     /translation="MRISKLKSAFLLILLSLFIQSTLLPAAAATASVCRSVFAYQSTQ
                     AEWQRLSQEERYSRRKAEMHTPSRQGVIERRPIIKTSGLRKDAVPDIDPADIKVDTAD
                     PSLVMPITYPNGQTKYRINFSGLRFIEADSPADLYKPGIRKVRHSRGFYKDGTEMTGA
                     YHNEAWPWDVVIYKNDAGKWIALGGVMEQAVPGKLPNVRENNRTRSRWWGKVSHVQIK
                     PGVYEEHIVWQGPIHDFNISKHQGWKYHGYGGTLVTQFNPKTGMHEPIKNKRGNYILM
                     YERVIEERNGMPWMTSTFMRDMDPSLTHTVGPEIEVTNIISPVTGQIFEAFRRGDEKE
                     TTGYLREGDNVLKEKEHNQYIKAGSGNDYVRKYGIYLDYRDPGRDAQGLFKNVIDEKG
                     QLIDFASTLKLREIFNGTWVGRPQLSYDPQGKLWLAFHFVPKDSIPPGGPQEGWPSAE
                     DFPKYARIMAMFPVKFEYDKSRQPLLVKDIDPKYAYKYE"
     gene            complement(10274..11485)
                     /locus_tag="VIG33_06085"
     CDS             complement(10274..11485)
                     /locus_tag="VIG33_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270440.1"
                     /translation="MDGQMKYVPSIFTRMFSLFIFFYSLQSYAYFVAYLGNLESFSYD
                     RPTHILLSGLGTDLGTLPQLSALGKAARYHSLFPDRQVILISVFENAKNPAALTSQGF
                     EFLARNTFTLNTGTALRRLLKFSRIQSLEIFGHNSPSLGTQTDGPGERFDANYPGLSK
                     LRSRFTADSYASIHGCNSGWLMAPQLSRLWGIPVAGAFTGTRFERLHSDGHFYVYEKG
                     KAPNDNWATKNLVGGSCLAGGCVRMRPMFSPYNGDWGDFSTSPLVNFYKFFCPQNSVE
                     ECEKRMALSMLAFVSDKQVSLSHSLEEFKDQVKSFLCPVYANRKITLQCFEALEQSLR
                     GGSKAVHFTVNQKQLSCDLKTCNAQMTCTDDGCVVEQVNAGHPEPAVTMVEEFNHYLN
                     GFLKLQSPEIK"
     gene            complement(11656..13047)
                     /locus_tag="VIG33_06090"
     CDS             complement(11656..13047)
                     /locus_tag="VIG33_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270441.1"
                     /translation="MKIKYKLSEQEFYNINQSLYQEIANRASIYSFLFALLFSLYGLN
                     TSQIILKFEPGVSIWSNTWPRFLFNGVPLVLLGYYLKNSRHSAHFKALLWAIVQPLIL
                     VIASFIYVWPLMYNGNLEIYKYFHAANVFVIIFGLTFVAPSRTVTLVHVASYVLLFLS
                     PLLYLVRKDSELLLIVLSDFTGTVIGGCVAGHLTFQLRKKIALMDAHIKSSLKPLLGN
                     MITSAVYNNNLELLDNKSTFGVILSMDLRGYTQFMHNNSKEITGPFMKEYHFLVSTTV
                     GKFNGFIHKTTGDGHLISFGLMDQTENLSDILSIKNEVQDAENNKGKILTEMAIKVFE
                     DLVEQYENLKIKYDVCDELNIGGALAAGHIETQIQGDETFRREVDIGGDTILRSARLE
                     AYTKPLMEKIGIQCSMLILSPELIDNLKTNIDFDYWQVVDKDIAVRDFPAIKKVYYRI
                     WKYKLGRPVRIAS"
     gene            complement(13031..>13921)
                     /locus_tag="VIG33_06095"
     CDS             complement(13031..>13921)
                     /locus_tag="VIG33_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270442.1"
                     /translation="LLPFCLLLIIWCGAFLLAVTPIILLQPKTMVETYILYACVPILF
                     LLLFISIAAGLSRFAQRGIIAGTFPRDPFHPVYLLRRIYGICWTQVFYFKPLYAIALS
                     IPLLKKYLFRAFGYRGNTDFVIYPDTWIRDLPLLNIGKGAYLSNRATVGTNICLPAGT
                     ILVDAIEMEEQSLVGHLTVLAPGVKIGRKVEIGVCSTIGIRTKLAIGSKVNPCCAINH
                     GVVIGEEARVGSMSYIGVKTTIGPRLVIPDGANIPSGFSLNTQEEVAKFFQEETRKID
                     SYVSKLKERLNENLKNENKI"
BASE COUNT         4050 a         3144 c         2923 g         3804 t
ORIGIN      
        1 agccccagct tcagtcactg aaaaattaat catttctaaa gtcggtgatg atgtatccct
       61 tttgaaaccc ggaactgagg tccgcacaat tgggggcagg ccctggcagg aatctcttca
      121 agaagctgtt cgtttcactt ctggtagcac ggatagaatg cgccgtaatc aagccgcaaa
      181 atggttattg ccgtttatcc taggaacgaa agaaacattt aaagatctgc caattgaagg
      241 aattctcaac ggacaagtgg ttaaagaaaa tatttctcgg gttttgactt tatctgatgg
      301 aaatgcagaa ccccctcctg cggccaccgg aaagggaagc atcaacgcca gaattattaa
      361 tggcaatatt ggttatttaa gaattgatgc tttcatggga accaaaatgg acatattatt
      421 ggatcaagcc atgagtcgat tgctccacac tgagggtcta ctcatagatg tgcgcctcaa
      481 tggtggaggg actcctgttg gcgatagagt gctgtcttat ttgatcgaac aagaaatcat
      541 gcgatattcc gctaaaatca gaaattcaga tatgctgatg gcgatgcagc ccctctatgc
      601 aacagggttt gactttataa acgggaattg gtccgaagtt tggcaatata aagtgcagcc
      661 aaatgcccac ggcaaatata aaaaaccagt cgttgtcttg acgtcggctt attgtttcag
      721 tgcctgcgat acgtttgttt cggccctcaa agagaataac ttagccaagg ttgctggtga
      781 agctacgggg ggaggaaccg gaagcccagt ggttttcaat cttccttatt cgaaatttaa
      841 atttcgatac tccgtacaaa aaggttacaa agcggtgtcc ggtgatttac tagaaggcgt
      901 tggaaccggt gtcgatctcg acatctctcc tacggcacaa gagcgaatcg ctgaaaaaga
      961 tgaacagtta aaaacagcgg ttgcttattt gaaaagtctc atgaccgacc agccgacact
     1021 tcaattccat ttaaattcag tggcagaaga aaactcggag tttgaaaggg ctgctgaccg
     1081 tcagccttta cgttcaccac tcttggagat ggagtcggaa atcctgaagt cgaactttga
     1141 aaattagatg aattgcccct cttctcatct ttttgtgttt gagatctgtt gatcgatttt
     1201 tcgctgagtc gtctcagatt ttcgtgtact ggcctgcgat ttgaaagttc gcagtttcgc
     1261 ctttttaatt ttcttttttg cttcccgttg gatgactttt aattctatgg cagcctcccg
     1321 cagtctccgc ttcgcctcaa tggcctcttt ctgggccaaa tcagcctctg cgttggcttc
     1381 gctgaccata tgctcttgat ctttgatttg actttctaat ttttccagct cgcgcaaagt
     1441 gtctcttttt ttatcaagga tttgatcacg gtgctggttt gccttctgta atcggactct
     1501 cgctctgtca attttctcct ttgctttagc ttgctgggct tcggcgattt ttatttgctt
     1561 ctcatagcct tgcgcttccg cagcgacttt agcggactct tttcgcgcca gggtggcttc
     1621 tttttccgct tgcgcctccg cccctttggc ttctttgagg gttcgccggg attcgatttt
     1681 caattgctct ttccgtttct tttcatcctc cgccattttc ttcgcttcag cggcgtctat
     1741 tttggctgca tcggaatcta ctatggatcg ttcgacgtcc acttctaatt ccgagctctc
     1801 ctggccaaaa cagatttgag tcaggaagat aagaaataga ataggaagca tatatttcat
     1861 caaatccacc tcaatgagtt ttacagatgt tagggcccat agtttaaaga cgtatagcca
     1921 gctcctttcg tttcggttat ttcaaataaa taaatgagat cttttaataa actcttgtat
     1981 tattttgaga cgctttttgg atttcctcaa gtagtctgct ttaagacggc tacgctcctg
     2041 cacttgaaat gacgaccgta ggcttgtata aacgttccaa aagggatgac tattatgtcg
     2101 aaagatgcaa ctgaaacagt gggcgatcat tttcaaaatg atttacttaa attcgctcga
     2161 gaccagagtt tggctgtgat tttgaaagcc tcttcttaca tcaagccagg aatccgggaa
     2221 aacgaagcta aagccctcat tcaagagatt caaacgcaga tgggggcccc gaaatcgtgg
     2281 catcctccgc aaattcgatt tggtgaaaat acactgctgc cctttgggca acaaggccat
     2341 gaaaatctga gcttgaaaga aaatgacatc tacttttttg atatcggtcc cattttttat
     2401 gggcacgaag gtgacgttgg ccgccccttt gttgtgggca atgactctga aatgaaaaag
     2461 tgctgtcgag atgccgcatc gatctggcag gaagtgagag atcactgggt atcaacgaat
     2521 gccacgggaa aagaactcta tcaagtggcc cggtttagcg cagaaaatcg cggctggctg
     2581 ctttcattac atgaagcgaa cggacaccgc atctctgact ttccccatgc cgcaagaatg
     2641 cggggatccg tagagaaatt cgaacaaaag ccagcaccta acagatggat tcttgagatc
     2701 caaattcgcc atccgaaaaa atcttatggc gccttttacg aagacctgct gatctaaagg
     2761 aagaatgaat gaaggaagct gctagcttcc ttcatcgccc ttaacgtctt aggcttgttg
     2821 ggtttcacaa gaccgattgc gtttcaagct acggtctcta ccttcatccc tacatcaagg
     2881 gcatcttttt ctttacaaga tgattatgca ctttcgggtt ccagacctca agcctatttt
     2941 ccgcttgaat cgatcctctt tttgcgacaa tttcaatcct taaaaccgct gtcattacaa
     3001 atgcctaatt gatttaaacg ggattaaaaa tgattaagga gcagcaactg ttgtggaaac
     3061 tgggcagaaa cgcacttgtg catgcaaacg gcccatacta tctacctgcc agaagtactc
     3121 tgataatctg ccatcaaaaa gtggaacccg ctcgacaata ccatcggcgt taatgtcggc
     3181 ataaatataa agaagttgct ggctgacatt taaaaatttt gaagatcccg cgatacgaga
     3241 catatttact tgatcaccac tgcaataggt ttctcccaag gcgtccgttc cacaggtggc
     3301 catattaata ccgctatgag gtttgcctac aaggcgcata taaactgaat aggcagtctt
     3361 tcctgagttc gttgggtctg gagccggaag cgagaaagac gcctgtccat cagtcccatt
     3421 gcgatcaata atattaaagg gcccttcttg tagcaaaatt ttagtgcgtg tattcagggc
     3481 aataaacaaa gcgcgtccat tggttcccgt catatccgca gtctttgatt tatgagcgat
     3541 gatttgcata ttgtagcaat tgccgcccgt gttaccccct cccgccgcga gagcggaatg
     3601 gtttgcaagg atacccatgg ctgccatgac caatcgacta gatagtttag ataatttcat
     3661 atgatctcct agtttgttta ttttctcgac gactcgacgt tcaagtcctt ctagcttttc
     3721 cgtgccgtca cttttcatta aggcaaggat caagccaaat taaaaaaact gaatatcaaa
     3781 tatttcaaag acttatattt tacgaattta actcaaagtg ttcagcacta aggatcttaa
     3841 gctcctcaaa agcgcgaagt gatgaaaagt attaagtttc ttctagaggg cagaaaactt
     3901 ggacagtgtc catgaagtga cgatcttaaa aaatttaaga cacaagaatt tatttgcttt
     3961 gaagcctgtc ttcgcctaac tctttaattt aattaacggc gtttctatag acatcacagg
     4021 ataggtctca tgaattcaaa ttacattcct cgcaagcttc tctttggcaa tccagatatc
     4081 agagacctta aaatcagccc cgacgggagg catatagctt accttgctga acatcatggc
     4141 gttctcaaca tttgggcgca ggatgttggc aatgagtcca ctgcgcgacc tctcacccac
     4201 gacactaaaa gaggtatttt ttcttatgat tggaccttcc aaccggggac cctgattttt
     4261 agccaggaca tagacggtga tgagaacttc ggcctctaca cgctgaatgt tctcaatggt
     4321 gaaacgaccg aaatcactcc ccctggtcac tttaccactc atgtcagtga gctgagccat
     4381 ttaagacctg atgaagtggt tatcctcaca aacaatcgcg accctatgta ttttgattac
     4441 aaaatactgg acctgaggga taaaacccaa aaagaccttt tcactaatac cgagaacttt
     4501 gccctcatcc atttcgataa atatttccga cctgtgatag caatcaaaag caatgccgat
     4561 ggctcctata caggttctct gtggaaccct cataccaaaa gtttttttga aaaattcgta
     4621 acggcttttg aagacagcat gtcttctaca accatttcaa cctctaacga cgggcggaag
     4681 gtgtaccttt taagcagcga aggaagagat aaggcgacat ttcaagaatg ggatctagcg
     4741 acggacacac ttactgtgct tgctgaagac tcaaagtctg atatccaagc catccaaata
     4801 catccgggct ctggcaagcc cttgatggcg acttcatact atgttaaagc gagaggtcca
     4861 cttcttggat gaggaattcc aaaaacatta taagaatatt caaaataaac ttcaaactga
     4921 attcaaaatt acttctctga gtctttcgct tgaacattgg gtgatcatga cggacgaggc
     4981 ccataaaccg gctgcttttt atttttttaa tactaaaact caaactctca gcgcccccct
     5041 gcatactcgc aaatctttgc ttccacatac agcgaaactt caacccatgg aatgccttga
     5101 gatcctttca cgggatggat ttcccttggt ctcttattta actcgcgcca aagaacaagc
     5161 tggcaaagct ttgattctct tggtccatgg aggcccttgg tcgagggatc gctatggttt
     5221 taatgtctac caccagtggc tggctgatcg tggctataat gttttgagcg tgaacttccg
     5281 ctcttccgtg ggatttggta aaaattttct gaatgccggc gatcaacaat ggggtcacaa
     5341 aatgcatgag gatcttctgg atgccgtcaa ctgggcggtg aataaaggac atgcggatag
     5401 cagccaagtc gttatcatgg gcgcctccta cggagggtat gctgctttgg cgggactgac
     5461 cttcacgcct gatacctttg ctgcggccgt agatattgta ggtccctcaa atctagagac
     5521 tctcctctcg accattcctc cttattggga aagtttcaaa gcgaatctct atcgacgcgt
     5581 cggagacccg acgacggaag cgggtcaaaa acttttgaaa aagtgctcgc ccttaaccca
     5641 tgtggataaa ataaaaaaac ctctgctcat tcttcaagga gccaatgacc cacgggtaaa
     5701 aaaagcggag gccgatcaaa ttttcaattc aatgttgtcc aaaaagattc cagtacagta
     5761 catcctcttc cccgatgaag gccatggctt tgcaaaatct cagaacaaca tgggagccaa
     5821 tgccatcatt gaaggttttt tgcagaagtt cttagggggg cgcctggagg acattgaaga
     5881 tgacctcaaa gagtcctcag cccagtttat aactgctctg caataggctc ttgattttca
     5941 ttgcccgagt tttattccat ataaagcttg gactcgacac atttcttcga gttcttatgc
     6001 cgaaaacaat aaggggaaac ccttgttgtt ttccaaattc ctatttcctt attttgggcc
     6061 tgcatcataa gcaggttata ctccccatga ctgtgtttcg gcgagtccca agcaaaacca
     6121 ttccttacaa tctcttcgtt gatatttttg gattcgtaaa aaacgataca ggccaatcta
     6181 tcaaatgaca ttccctcaca cttaagatcc acaattttat gcaaaataag actttccgta
     6241 aagccttttg ccatcggtcc ttcaggttgt ttaatttctg gcgtatcgat tccggaaaat
     6301 cttatcttga gcgatttatt gttcactaaa actcgacagg tatccccatc atggcaactt
     6361 tgcacgaaac cccgaaaatt ttggatttga tttttctttt tagactttgc aagagaaacg
     6421 gaagaaaagc ctattgaaag agcaagaacc atgagaaacc aaattttcat cgatgcggcc
     6481 ttgttgaagt taaaaaataa ttttttaact tttagactta aaagtaaagg gaatgatgag
     6541 cccgagttgg gccgattcaa taatttctaa atcttatgtt tcgcaactct ttaccataag
     6601 accttcgccg tcttgctgat cggcaaaaca gccgatcaga tctttatttc tacttaaaca
     6661 attttcctat ttttaattct tctttgattt gaaactgcaa ctgaattggc tgcacgcagg
     6721 gacatgatat gaatttggag ctgagtattt aaagccaaca acaaaacaaa acaaaataaa
     6781 aggagaaaaa atgaaaacaa tttcgtggat tacaacaatg ttatttgcgg ccagcgctta
     6841 tgcacaagcg ccgatgtcag tcatagatag ccctgacatc aactcatctt cccaaaacga
     6901 agaagcttat caagagtatt atgatcaaga ttactcagat caaaactatt ttgatcagga
     6961 gcaaaacaat tccgaagata aaagttctga gagtaataaa gaaccagaat tcaccccaac
     7021 ccaaagatcc ggtactcccg gttatatcgg gcgcagacct ggatatcccg gtggcagact
     7081 tgttcgtggg cctggtggac atatcggcgg cccctgaata tcgtcgtggc tggtattcac
     7141 gttatcgttg ggaccgaggc taccatcgtc cacgctggtg gagcgctcga catcgcttcc
     7201 cttcttttgc atggattctc aatattggag tcggttactg gtagtgtacc gccttcaact
     7261 cctcattata tccttactcc gcattgggac gcagtataga tgaggctgct tacaatgcta
     7321 tgtatgattg cggtggcaca cgagccgcat ctcgctgcta tatcccccct ggctattgca
     7381 accactacta tccttaagaa cccttgcact attgaagtga tatttaaaat gataagaagg
     7441 ggccaccgag cccctttttt tcttctccca ccagacccat taaaaattaa ctatttgttc
     7501 gatgacaaat ggcaagttag tatgaaatcc aactcagctg gatgggatga cttatgcgaa
     7561 cgggacaaat ttttgaagtt attggaaaaa caccacttat aaaaataaaa tcactttctg
     7621 aagctactgg ctgtgaaatc tatgggaaag ccgagttcct aaatcccgga ggaagcgtca
     7681 aggatcgggc cgctttagga attatttccg aagccgaaaa gctcgggcaa ctgaagagtg
     7741 gctatacgat cttcgaagga actgcgggca atactggaat tggccttgcg accctcgccc
     7801 cccaaaaagg ctatcgttgt gtcattgtga tgcctaacaa tcaatcccag gaaaagtatg
     7861 atgccctgaa agccctggga gtggaattaa tcacggttcc tccctgcccg tttaccaatg
     7921 aaaatcactt ttaccacact gccaaaagat tggccactga aaggcctgat agtttttggg
     7981 ctaatcaatt tgaaaatacc gcaaacttta aagcccatta tcaaaccacc ggccctgaga
     8041 tctgggagca aatgaacgga aatattgatg cctttatctc agctgtaggc accggcggaa
     8101 ccctttccgg tgtttcgcgt tttttaaagg acaaagacaa gtcagtgcat attcgcttag
     8161 tggacccttt tggctccgga ctttattctt atttcaaaac aggacaactc tcttcaacag
     8221 gaagctcgat cactgagggt attggtatta tgcggctcac ggccaacttc aaagaagccg
     8281 ttatcgacga agccgttcag gtgaatgatc aagaaatgct ttccactctt tatcacttgg
     8341 ccgaacacga tggtttgctc gcgggaactt ccgcggcatt aaatgtgtat gcttcttata
     8401 agtatgcttt agaaaataaa aattctggaa aacgaatcgt gacgatcttg ggggactcag
     8461 ctcttagata tcaatcgaag atatttaacc ctcaatttct ctcagaaaag ggactcttta
     8521 agaagacctt gctttcatct tgataaatcc ttttcctaag aattgttcat gaaaatttta
     8581 tgcgacaact ctttcgacag gaacacgaat tggcgaccct gtctagactc tctccaacaa
     8641 cccgcactta aagtcgcagt gataaaatct ccatagcagt gaagacgaac agacctgggc
     8701 acatttcttg aatgacttga aaaaagaaat ctcacgaaag aggcatcgtc aaagttgacc
     8761 aggaagggcc catgcgaatc tcaaaattaa agtcagcatt tttattaata ctgctctcct
     8821 tatttatcca atccaccctc ctcccggcag cggcggcaac cgcttcggtc tgtagaagtg
     8881 ttttcgctta tcaatcgacg caggctgaat ggcaaagact ttcccaagaa gaaaggtata
     8941 gtcgtcgcaa agcggaaatg cacactccct ctcggcaagg agtcattgaa cggagaccta
     9001 ttattaaaac ttcaggcctt cgtaaagatg cggttcccga tatcgaccct gccgatatca
     9061 aagtggatac agccgatccc tctttggtaa tgccaatcac ttacccgaac ggccagacca
     9121 aatatcgaat caacttttca ggattaagat ttattgaagc cgacagcccc gcggacctgt
     9181 acaaacccgg catcagaaaa gtccgacatt cacgaggctt ctataaagac ggaacagaaa
     9241 tgaccggcgc ctaccacaat gaagcctggc cttgggatgt ggtcatttat aaaaatgatg
     9301 ccggtaaatg gatagccctc ggcggcgtca tggaacaagc agtgcctgga aaactcccca
     9361 acgtcagaga aaacaatcgg acacgctctc gatggtgggg aaaagtttcc catgttcaaa
     9421 tcaaacccgg tgtttacgag gagcatattg tttggcaagg tcccatccac gactttaata
     9481 tttccaagca tcagggatgg aaatatcatg gatacggcgg aaccttagtg acccagttca
     9541 atccgaaaac aggaatgcat gagcctatta agaataaaag aggaaattac attctgatgt
     9601 atgagcgcgt aatagaagaa cgcaatggaa tgccttggat gacttcgact ttcatgcggg
     9661 atatggatcc ctctttaacc catacagtgg gacctgaaat agaagtgact aacatcattt
     9721 ctccagtcac cggacaaatc ttcgaagcct tccgtcgagg cgatgaaaaa gaaaccactg
     9781 gttacttgcg cgaaggcgat aatgtactta aagagaaaga acacaatcaa tatatcaaag
     9841 ccggaagcgg caacgattat gtacgaaaat atggaattta tctggattat cgtgaccccg
     9901 gaagggatgc tcaagggctt ttcaaaaatg ttatcgatga gaaaggtcag ctgattgatt
     9961 tcgcatccac actcaaatta cgggaaatat ttaacggcac gtgggttgga cgcccacagc
    10021 tgagttacga tcctcaagga aaactctggt tagctttcca cttcgttccg aaagacagca
    10081 ttcctcccgg cggaccccaa gagggatggc ccagcgctga agactttcct aaatatgctc
    10141 gtatcatggc aatgtttcca gtaaagtttg aatatgacaa atcaagacaa ccgcttttag
    10201 tgaaagacat cgatcctaaa tacgcctata aatatgaata gaggtggcga gatcatccgt
    10261 acgataacaa gacttatttg atttcaggag attgcagctt aaggaagcca ttcaaatagt
    10321 gattaaattc ttcgaccatg gtgaccgctg gctctggatg gccggcattc acttgttcca
    10381 caacgcagcc gtcatccgtg caagtcattt gagcgttgca agttttaagg tcgcaggaca
    10441 gttgcttttg attcacagta aagtgcactg ctttactgcc tccccgcaaa ctctgctcaa
    10501 gggcctcaaa acactgaagc gtgattttcc ggttggcata gactggacag agaaaagatt
    10561 taacctggtc tttaaattcc tctagagaat gacttaagct cacttgctta tccgacacaa
    10621 aagccaacat tgatagtgcc atgcgttttt cgcactcttc aacggagttt tgaggacaaa
    10681 agaatttata gaaattaaca agtggtgaag tgctgaaatc accccagtcg ccgttataag
    10741 gcgaaaacat cggacgcatt cgaacacacc cccccgctaa gcaagatcca cctacgagat
    10801 ttttagtcgc ccaattatca ttaggagcct tgcccttctc ataaacgtaa aaatgaccat
    10861 cggaatgcaa gcgctcgaaa cgagtccctg taaaggcccc agcgacaggt ataccccata
    10921 acctagagag ttgaggggcc atcaaccaac cactattgca gccgtgaata gaggcgtaag
    10981 agtccgctgt aaacctgctt cttagcttag agagacctgg ataatttgca tcgaatcgct
    11041 caccgggacc gtctgtctgc gtccctaaac tcggagagtt gtgcccgaaa atttccaaac
    11101 tctgaatgcg cgaaaatttt aacaatctcc tgagcgccgt tccggtattg agggtaaaag
    11161 tatttctcgc taaaaattcg aagccttgag aagtcagggc cgctggattt ttggcatttt
    11221 caaaaacgga aatgagaatc acctgacgat cgggaaaaag actatggtaa cgagcggctt
    11281 tgcctagcgc agacaattgc ggcaaagtcc ccaaatccgt tcccagtccg gaaagaagaa
    11341 tatgcgtcgg ccgatcatag gaaaaactct caaggtttcc tagatatgca acaaaataag
    11401 cgtaagattg cagactataa aaaaatatga acagactaaa catgcgagta aaaattgaag
    11461 gaacatattt catctggcca tccataacct tcctcatcac tcgacaacgt ccactacgta
    11521 atccagttta aagtttatcg tcagtcggca aaacaatcaa gaaatgcgct tctacttggt
    11581 ctagttcctc actaacgatt tcatcactgc ccaaagggct gtccgtgatt atccgtgctg
    11641 gaggctaact ctattttaag aggcaattcg tacaggacga cctaatttat atttccatat
    11701 tcgataataa acctttttga tggcaggaaa atctcggact gcgatatctt tgtccacaac
    11761 ctgccagtaa tcgaagtcaa tattggtctt caggttatca ataagttcgg gtgaaagaat
    11821 gagcattgag cattgaattc ctattttttc cattaatggt ttggtgtaag cttcaagacg
    11881 agcgcttcgc agaatcgtat cgccaccgat atccacttct cgacgaaagg tttcatcccc
    11941 ctgtatctga gtttcaatgt gacccgcagc cagggctccg ccgatattta actcatcaca
    12001 cacatcatac tttattttca gattttcata ctgctccaca aggtcttcaa aaactttaat
    12061 agccatttca gtgagaattt ttcccttatt attttcggca tcctgaactt catttttgat
    12121 agataaaata tccgaaagat tttctgtttg atccatcagc ccgaacgaaa tcaaatgacc
    12181 atcacccgtt gttttatgga taaatccatt gaacttaccg acggtggtgc tgactaaaaa
    12241 gtggtactcc ttcataaaag gtccggtgat ttcttttgaa ttattatgca tgaattgagt
    12301 atatcctcgc aaatccatcg acagaatgac accaaaagtg ctcttattat ccaagagttc
    12361 taaattgtta ttgtaaaccg ctgaggtaat catatttcca agcaagggtt taagactgga
    12421 tttgatatgg gcatccatta aagcaatctt ttttcttaac tggaaagtga gatgtccagc
    12481 aacgcaccct ccgatgaccg tgccggtgaa atcgcttaga acgatcaata agagttcact
    12541 atcctttctg actagataaa gtaaagggct taagaacaac aaaacatagc ttgccacatg
    12601 gacgagagtg actgtgcgag agggagccac gaaggtaaga ccgaaaataa tcacgaaaac
    12661 attggcggca tgaaaatatt tatagatttc cagattgccg ttatacatga gcggccatac
    12721 ataaatgaaa gaagcgatga caagaataag gggctgcaca atggcccaaa gcaaggcttt
    12781 gaaatgagca ctatgtcttg aatttttaag ataatatcca agaagcacca agggaactcc
    12841 attaaagaga aaacggggcc aggtgttgga ccaaatcgat acaccaggtt cgaactttaa
    12901 aataatttgt gaagtattga ggccatacaa actaaagaga agagcaaata aaaaggagta
    12961 aatagaggcc cgattagcga tctcttgata gagactttgg ttaatattat aaaactcttg
    13021 ttcgcttaat ttatatttta ttttcatttt ttaaattttc gttaagacgt tcctttaact
    13081 tagacacgta ggaatcaatt ttacgagttt cctcttgaaa aaattttgcg acttcttcct
    13141 gagtattaag agaaaagccg gagggaatat tagctccatc ggggatcact aaccttggac
    13201 cgatggtggt tttgactcct atgtaggaca tacttccaac tctggcttct tcgccaatca
    13261 ccactccatg attgatggca caacagggat taaccttact tcctatagca agtttggttc
    13321 gaataccaat ggtcgaacac acaccaattt ccactttcct tccaattttc actcctggcg
    13381 ccagaacggt taaatgacct acgaggcttt gctcttccat ttcaatggca tcaaccagaa
    13441 tcgtccctgc gggaagacaa atatttgttc ccacagtagc tctattggac aagtaggccc
    13501 ccttgccaat attcagcagg ggcaaatctc gaatccacgt gtctggataa atcacaaagt
    13561 ctgtattgcc ccggtatcca aaagctctaa agagatattt ttttaatagt ggaatgctca
    13621 aggcaatcgc atagaggggt ttaaaataaa atacctgagt ccagcaaatt ccgtagattc
    13681 tacgcaataa atagacaggg tgaaaagggt cgcgggggaa tgtccccgca atgattcccc
    13741 gctgtgcgaa acgactcagt cccgcagcga ttgagataaa cagcaataag aatagaattg
    13801 gaacacaagc ataaagtatg taagtctcaa ccatcgtttt gggctgtaaa agtattattg
    13861 gggtcactgc gagaagaaag gccccgcacc aaattattag caataaacaa aaaggcaaaa
    13921 g
//
LOCUS       DATBTE010000160         2157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_156661, whole genome shotgun sequence.
ACCESSION   DATBTE010000160 DATBTE010000000
VERSION     DATBTE010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156661"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /gene="mnmE"
                     /locus_tag="VIG33_06100"
     CDS             complement(<1..1148)
                     /gene="mnmE"
                     /locus_tag="VIG33_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEY8270443.1"
                     /translation="MNGGDRDSQTICAVSTPHGVGGISVIRISGPNSLEVTRKIAKFL
                     PQKIESHKIYFGTLQDFQSRENVDEVLVAYFQKGHSFTGEEVVEISCHGNPQICQNIL
                     NLLITGGARSADKGEFTYRAFMNGKVDLVQAESILALIESQSEKAQKLALRQLKGQLS
                     QKLERIEDQLTWLMAHIEAGIDFSTEGLDLVDTEIIKEKLVEIETSLKSLVNSYKAGR
                     ILKDGVRIALVGSPNVGKSSLLNLLLEEDRAIVTAIAGTTRDVISGDTKYDGLKLTFL
                     DTAGIRNHTSDIVEQIGIQKSREAYADADVVLFVFDLSVGVQPQDEELLRHLSKTNLF
                     VIGNKLDQINNPTETPGFEKITHYADFAKRNLFFVSALDQNARPLMLK"
     gene            complement(1155..1718)
                     /locus_tag="VIG33_06105"
     CDS             complement(1155..1718)
                     /locus_tag="VIG33_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEY8270444.1"
                     /translation="MAGFFSKLFGGKNKSSGGEVETLVNKTLAGIIEKGQFDLSFEVS
                     YEKEANTEAVLAIEISGSDEELLKDKKGQMLDALQLFLKRAVQHNFPEDKTNITVDCG
                     GYREESAAALIERAENLKTIAIEQGKSVYYRALPPKDRKVVHQYLAQDARVKSRSLGE
                     GLYKKIKIYPAKGGTNQGNENHESAGE"
     gene            complement(1727..>2157)
                     /gene="yidC"
                     /locus_tag="VIG33_06110"
     CDS             complement(1727..>2157)
                     /gene="yidC"
                     /locus_tag="VIG33_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY8270445.1"
                     /translation="KDDPMALNRETMAVMKEHKANPIGGCLPMLLQIPVFFALYRVIG
                     SSIELYHSPFFGWITDLSAPDKFFVLPILMGGTMFLQQKMTPTTMDPAQAKIMAFLPL
                     VFTLFMLQLPSGLTLYMTVSAIFGIVQQYIFLKDSKKVTA"
BASE COUNT          642 a          494 c          377 g          644 t
ORIGIN      
        1 tctttcaaca taagtggtct cgcattttga tcgagagcgc taacaaagaa aaggtttctc
       61 ttggcaaaat ccgcatagtg cgtaattttt tcaaaacctg gagtttcagt gggattattg
      121 atctgatcaa gtttattacc aataacaaaa agattggttt ttgacaggtg ccttaaaagc
      181 tcttcatctt gtggctgaac tccaactgag agatcaaata caaatagtac gacatcggca
      241 tccgcatagg cttcgcgact cttttgaatc ccaatctgct cgacaatgtc tgaagtatga
      301 ttgcgaatcc ctgcggtatc taaaaaagtc agtttaagtc catcatattt agtatccccc
      361 gaaattacat cacgggtagt tccagcaatc gcagtcacaa ttgcacgatc ctcttctaaa
      421 aggagattca gaaggctaga ttttccaaca tttggactcc caacaagagc tatccgcaca
      481 ccatctttta agatcctgcc ggctttatag gaattcacca gagatttgag agaagtctca
      541 atttccacaa gtttttcttt aataatttcg gtatctacta aatcaagccc ttcggtcgaa
      601 aaatcaatcc ccgcctcgat atgagccatc agccaagtca gctgatcttc aattctttct
      661 agcttttgag aaagctgtcc tttaagttgg cgcaaggcca atttctgagc tttttcgctt
      721 tggctctcga tcaatgctaa aatgctttca gcctgcacta aatctacttt gccattcata
      781 aatgcgcgat aagtaaattc acctttatcc gcactccgag caccacctgt gatcaaaaga
      841 tttaaaatat tttggcaaat ctgtggattt ccatgacagg aaatttctac aacttcttct
      901 ccagtgaagg agtggccttt ttggaaataa gccaccagaa cttcatcaac attttcacga
      961 gactgaaaat cttgaagtgt tccaaaataa attttatgtg attctatttt ttgcggtaaa
     1021 aatttcgcga ttttacgggt cacctcaagg ctattaggcc ctgagattcg gatcacagag
     1081 attcctccca cgccatgagg agtggatacc gcacagatcg tttgagaatc cctatcgcca
     1141 ccattcatta aatactactc tccggcagac tcgtggtttt cattaccttg gttagtccca
     1201 ccttttgcag gataaatttt aattttctta tagagtcctt cacccaaaga acggctctta
     1261 acacgggcat cttgtgctaa atattgatga acgactttgc gatcttttgg aggaagcgct
     1321 ctgtaataaa cagactttcc ctgctcaatt gcaattgttt ttagattttc tgcacgctct
     1381 attaaagccg ccgcagattc ctcacgatat cccccgcaat caacagtgat attcgtcttg
     1441 tcttcaggaa aattatgttg aactgcgcgc tttaaaaaca actgcaaagc gtccaacatt
     1501 tgaccttttt tatcttttaa aagctcttca tcagaaccag aaatttcaat cgctaagaca
     1561 gcttctgtat tcgcctcttt ctcataggaa acctcaaaag aaagatcaaa ctgccctttt
     1621 tcgatgatac cagctaatgt cttgttaacg agagtttcca cttcgccacc agaactttta
     1681 tttttgccgc caaacagttt actaaaaaat ccggccatat ttcctcctag gcagtcactt
     1741 ttttcgagtc ttttaaaaat atatattgtt gaacaatacc aaaaattgca ctgaccgtca
     1801 tatagagagt caatccactt ggcagttgaa gcataaacaa agtaaacacc aacggcaaaa
     1861 aagccatgat tttcgcttgt gcgggatcca tcgttgtcgg agtcatcttc tgttgtaaga
     1921 acatcgttcc acccatcaaa ataggcaata cgaaaaactt atcaggagca ctcaaatccg
     1981 taatccaacc gaaaaacgga gagtgataaa gctcaatact agagccaatc acgcgataaa
     2041 gagcaaagaa tacaggaatt tgaagaagca taggtagaca tccacctatc ggattggctt
     2101 tatgctcttt cattaccgcc attgtttcac gattaagagc catgggatca tctttat
//
LOCUS       DATBTE010000161         1901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2206678, whole genome shotgun sequence.
ACCESSION   DATBTE010000161 DATBTE010000000
VERSION     DATBTE010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2206678"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="VIG33_06115"
     CDS             complement(<1..1306)
                     /locus_tag="VIG33_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270446.1"
                     /translation="MTLFKKTSLFLMVFFIGYPILYLIQFWHVKNQSAQMDSERIATI
                     SQMLKHSSQQSTEDLFLNMFDVYLKNDAISYYVIRYQDGRLLYPSSEHALLKESQYKE
                     TNTFQSRNEITLGKQKYSVLYGKDDFPVLDQLLQLLKEQKEILFIFLLGIYLLIYKVA
                     AYFLKFDVGNIIQWFKNRSGTSDVEILSNDAQKLKTFIHDVEQRRTLAEALVQEYRDN
                     AYSGLTWAHEKMKSGLSSANITVGRCDINNFTVLKQKYSPELINYIVQSFFNRGGEYL
                     NRYGAYKESAAGDEITFFVPESASENSHLNALHVIRGLFEIFEDLKTELATIDQPLTA
                     KGSLDYGATELEKMGGNAETDGEPFIITERILKTVEDGEKNNSILVILSNTKDHYGNH
                     ISIHHESSEKIKNYENPKNISFIKTIKSYVSLNSVTSSNIYQI"
     gene            complement(1308..>1901)
                     /locus_tag="VIG33_06120"
     CDS             complement(1308..>1901)
                     /locus_tag="VIG33_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270447.1"
                     /translation="PTTTIRSVASVPQHEVNIVTPNLGTIPLPESHYQAVKRHVNTSV
                     ANISKKIAPVIAYVESPAFAAELPQSPNTNSDESAVRKTAAENPAKISQSNEAASPSR
                     EPATISTTISSGTTRNSSSGSTKVTIAKSQPRLTELGLDNAYALKVRKRLVVDENYRS
                     QLRQNGVNIDFGDGFKFESKEKNIQYIEKNGVLVRRK"
BASE COUNT          522 a          356 c          391 g          632 t
ORIGIN      
        1 ggatctggta tatattcgag cttgttacag aatttaatga tacataagat ttgatcgttt
       61 tgataaaaga gatattctta ggattctcat aattttttat tttttcggac gattcatgat
      121 ggatagaaat atggttcccg taatgatctt tcgtgttact gagaataacc agaatactgt
      181 tattcttttc gccgtcctcg acagttttta aaatcctttc agtgattata aagggctcac
      241 catcggtctc ggcatttccc cccatttttt ccaattctgt tgcgccatag tccagggatc
      301 ctttagcagt cagaggttga tctatagtag caagttcagt cttgaggtct tcgaagattt
      361 caaaaagtcc acggataaca tgaagagcgt tcagatggga attttccgag gcactttccg
      421 gtacgaaaaa agtgatttca tcacctgcag ctgactcctt gtaggcgcca tatctattta
      481 agtactcacc gcctctattg aaaaaagact gcactatata attgatcaat tcaggggaat
      541 atttttgttt aagaactgta aagttattga tatcacatcg gccaacagtg atattggcac
      601 tgctcaatcc ggatttcatt ttttcatgag cccaagttaa accgctatag gcattgtctc
      661 tatattcttg aactagtgct tccgcgaggg tacgccgttg ttcaacgtcg tgaatgaaag
      721 ttttgagctt ttgagcgtca ttagataaga tctcgacatc cgaagtcccc gatctatttt
      781 tgaaccattg aataatattg ccaacatcaa actttaaaaa gtaagcagct actttgtaga
      841 tgagcaagta aatgcctagc aaaaatataa aaagtatttc tttttgctct ttgagcaatt
      901 gcagtaattg gtcaaggacg gggaagtcgt ctttgccata aagaacagaa tatttttgct
      961 tgccaagtgt gatttcattc cgtgattgga aagtatttgt ttctttatac tgactttctt
     1021 taagcaaggc atgctcactc gagggataaa gcagtcggcc gtcttgataa cggataacgt
     1081 aataggaaat tgcatcattt tttaagtaaa catcgaacat atttaagaat aagtcttcag
     1141 tactttgttg gctgctatgt ttcagcattt gtgagattgt cgcgatccgc tcagaatcca
     1201 tttgtgcact ttggttttta acgtgccaga attgaatcaa atataaaatc ggatatccaa
     1261 taaaaaatac cattaaaaag agagaggttt ttttaaagag ggtcatttta ttttctcctc
     1321 actaaaacac catttttttc gatatactgg atattttttt ctttactttc aaacttgaag
     1381 ccatctccaa aatctatatt gaccccattt tgcctcagtt gcgatcgata gttttcatca
     1441 actacgaggc gctttcgaac tttcaaggca taggcattat ccaatcctaa ctctgtcaag
     1501 cgtggctgcg atttcgcgat agttacctta gtgctgccgg atgaactgtt tctggtagta
     1561 ccgctagaaa tagtggtaga aatcgttgcg ggttctcgag agggtgaggc ggcctcatta
     1621 ctttgtgata ttttcgctgg attctcagca gcagtttttc taacggcgga ctcgtctgaa
     1681 ttcgtgttgg gagattgtgg aagttccgct gcaaaggcag gactttctac gtaagctatt
     1741 actggcgcaa tttttttcga aatatttgca acagaagtgt tcacatgccg ctttactgct
     1801 tgatagtggc tctcaggcaa aggaattgtt ccgaggttcg gagtgacaat gttaacttca
     1861 tgttgcggaa cgctagccac agatctgatg gttgttgtag g
//
LOCUS       DATBTE010000162         1956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_20454, whole genome shotgun sequence.
ACCESSION   DATBTE010000162 DATBTE010000000
VERSION     DATBTE010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20454"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..826)
                     /locus_tag="VIG33_06125"
     CDS             complement(311..826)
                     /locus_tag="VIG33_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270448.1"
                     /translation="MKRSDTSQWNFGGWVFALTLQEFLFWGVLTNGYPSFLGHQFVGG
                     GIKGLAFPDTAADFIEKNQVTGNIFNETDWGSYLAWRWHGERKVFYHGHIDDPAFLKN
                     EYMKVYRSPAEFEDLVQKNDIKIFLMRTMRFTPGYRLTIKNLVDGMARGEWKLIYSDP
                     NASILIKTSIK"
BASE COUNT          658 a          388 c          383 g          527 t
ORIGIN      
        1 gcacttaatt ctgaatcaaa atgggattcc atttattcat gataattccg ccaccaatgg
       61 cccctcaacc tccggtttta tccctggacc atgggaccgg taaaccggtg aaaatggccc
      121 ctttttaaac cggcgcgtgc atgtggagag ggatgcaacg gttatctgaa attcagatag
      181 gggtcgagat cggaatgcaa tttgtgggta attgaaagga ggttcgacga tgtaccaaac
      241 gagagactca tctggatgtt cttacgtcac tgaactataa gacaaaactt aaacatcgtt
      301 atctaggctt ttacttaatt gaagttttta tgagaattga ggcattcgga tcggaataaa
      361 ttaatttcca ttctccacga gccattccat ccactagatt tttgatagtc aatcgatacc
      421 ctggtgtgaa acgcattgtt cgcattaaaa aaattttaat atcgtttttt tgtactagat
      481 cttcaaattc tgcgggtgaa cggtaaactt tcatgtattc gtttttaaga aaggctggat
      541 cgtcaatgtg tccatggtaa aaaacttttc tttccccgtg ccaacgccat gctaaataag
      601 acccccagtc tgtttcatta aaaatatttc cggtcacttg gttcttctca ataaaatcag
      661 cggctgtgtc aggaaaagct aaacccttta tgcctccccc gacaaattga tgacccaaga
      721 aacttgggta tccgttagtg agaactcccc aaaaaagaaa ctcttgaaga gtcagggcaa
      781 atacccagcc gccgaagttc cattggctgg tatccgacct cttcattaaa acgagttcat
      841 tgcttaaaat tagaagacta aaaaaagccg atacaagcat aaacctaaga gcagaggccc
      901 ccagagccac tatcagaaaa aaagcaaagt aggagaaaaa cgattttttc ttccagttca
      961 taatcaaaac aatcgcagtc acaatccaaa tgataagtaa cgccctcacc tcatttactg
     1021 atatggcatt ttgagcaaat gtgggcatcc actcagtgaa gtgagttcga aatatttgcc
     1081 agtctgggtt gaatattttt tggatcggat aaaagaaccc ctcccagcct agtggattaa
     1141 taaagcaagc tattgtagat aaaagaagac atttagttga caaggaaaat ttctcgctag
     1201 acccctttag tttttgtgca attaccgaga aaaataaaat taaaagtcca atatgaaatc
     1261 ctgggtgtac attactccaa atcaaaaata agagtggtat aagccaaaga aacacccatc
     1321 tcttatgaaa tggaagcaaa caaaccatca gtattaggac cgtaaaaaaa tcagaaaaca
     1381 aagaagcttt ttccgtgaag cgaatatttc cggcataagc agctattgga agtagaaaaa
     1441 agggaagaat cttttttccc cgtattgcag gaaatacgga gattcccgca ataatacaaa
     1501 ttaagcagga tttgaaaaaa atcagtgaat tgaatccacc aattatataa agaccataat
     1561 atataacgta agcgccccat tcgtgcatga tatgccacgg atattgagac cttgagtaga
     1621 gaaagggatc agttagtggt aggggcccat caaaaaatct acgtcccaat tctaaataca
     1681 taaaaagatc atcggagttt atcggtaaaa atgcggaaat agcaatagtc agcaaagcca
     1741 aaataaatgt cgaggctata gcaagatgaa taattttttg agagacccat acttttgaca
     1801 gcattgtctt aaattgtatt gcgagccttg tcatcggcac aagtctggat ggatctcagg
     1861 ctagacttta aacgcgctct agcctgtcga aatttttatg aactctgagg gataaatccc
     1921 ctttcgggat gattctggaa agttaacgat ccttgc
//
LOCUS       DATBTE010000163        14083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1524017, whole genome shotgun sequence.
ACCESSION   DATBTE010000163 DATBTE010000000
VERSION     DATBTE010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 14083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14083
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524017"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2877
                     /gene="gcvP"
                     /locus_tag="VIG33_06130"
     CDS             <1..2877
                     /gene="gcvP"
                     /locus_tag="VIG33_06130"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEY8270449.1"
                     /translation="SLKLSSHSTNQEFLGRHIGPREEDIQTMLKELGFSSLEEMVAQV
                     IPQKIRTTKKYPVVGDGVSEYELLGNLKKTLSKNKVYRSLIGMGFYDTITPSVIQRNI
                     LENPVWYTAYTPYQAEIAQGRLEALLNFQTMVIDLTGMEISNSSLLDEGTAAAEAMAM
                     AHASHKQSDVTHFLVSPKMHPHVIEVLKTRSEPLGLTMLIQNPKIYDFKTPVFAAFVQ
                     YPDTEGMIEDYKEMTQKFHDHQALVVAAVDLLSLTLLTPPGEWGADIVVGNSQRLGVP
                     LGFGGPHAAFLATKDAYKRIMPGRIVGVSVDSHGKPALRLALQTREQHIRREKATSNI
                     CTAQVLLANMASMYAVYHGPEGLKKIAQRIHRLTAVTAEGLKKLGFAIPTTPFFDTLT
                     VTTLKAAEIVKAAEALQMNLRVIDQKTLGLSLDETCNLQTVNDIFQAFNLGLKAPFTA
                     TEIDKDLNYSWPTAFIRTSKYLTHPVFNTHRSEAEITRYIHMLQNKDITLTHSMIPLG
                     SCTMKLNATTELLPVSWPEVNKMHPFAPTSQIQGYVEMIHDLENKLCDITGFAGISLQ
                     PNSGAQGEYAGLLVIRKYFIDKGEAHRNICLIPSSAHGTNPASASMAGMKVVVVNCDK
                     QGNVEVNDLKTKAREHKDNLAALMITYPSTHGVFEESIKEICQIIHDNGGQVYMDGAN
                     MNALVGLCRPAEFGADVSHMNLHKTFAIPHGGGGPGIGPIGVGEHLKKYLPTHSLQPE
                     AGPKTGISATTSAPWGSASILPISWSYITMMGAEGLKKATLVAILNANYIAKKLAPHY
                     PIVYKGKEGMVAHECILDLRDIKKVSGIDVSDVAKRLMDYGFHAPTMSWPVVGTLMVE
                     PTESEPKAELDRFIEAMVAIRKEITAVESGKMDLKDNPLVNSPHTVDSLLKAEWNHAY
                     SREHAAYPVSWLRQNKLWPAVGRIDNVYGDRNLICACPSIETYK"
     gene            2911..4509
                     /locus_tag="VIG33_06135"
     CDS             2911..4509
                     /locus_tag="VIG33_06135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY8270450.1"
                     /translation="MVMVMALASAFAIFIPQSLFASVPSHFLWSDSKGLMTVEATCQL
                     RHEEQNPFFISQKPNSQLNDYSKLKGLGFFGGWVSGKLLDRSLITAKEAPNAADDSQI
                     RVLSVAKNATRPDVLHISEAGDEGYLAKESLQDIGNFVIEIKDAPAFLNLKSPFRIQK
                     SFWQAATEDEKFLAFTCGDIAAPVRYFLFEVYDLAHLDPVAQVGVNLAETKIWDSINV
                     YTPEEANQVVKEEAEHTPPAPQPPTEVPLPSPQPPSEGQEDTPILEGELEYVICTEDK
                     TTGIFAQDLKTKIFSANQFETVIPSQSWDEKRKTTHVEVQFPAREEMPRSGWIPRDLV
                     QLRAECIPLKKTAPSDEDAPVDDSLTTLDPRVSRKDCCKFPTTQRSSQPYTSGILQFR
                     AKRNKGKRRHAGCDLYRSKGEAAVAVASGTVLRGLYYFYQGVFAIEVKHPKFIARYGE
                     VLGKYGPGVAKGKNVVAGQELGYIGKVNSGCCAPMLHFEMYKGSAHGPLTRHRVPPYD
                     RRSDVMNPTDFLKQWEKVQFGQSY"
     gene            4510..5193
                     /locus_tag="VIG33_06140"
     CDS             4510..5193
                     /locus_tag="VIG33_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043130 - ubiquitin binding [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CUE domain-containing protein"
                     /protein_id="HEY8270451.1"
                     /translation="MNKRFLLIIFGSLLLMSGIFFTAFSSRKPLTPETQVSVKSPVFH
                     EESPSTPPSKDLSPAKANAASFSLKEMSKILFQHTVTGGKLEDLVKDLKKTRQEPFLV
                     RDKNDETGEMIIVRTKSPLPGTRYFHAQYFTDETMQAFAQHMSFELHASPQAMNQAIQ
                     TVKEAFPNLDAPSEESRDFIQWDLEDGYVIWIKRLGAEDIQENPFNFYSAEDIGSIRI
                     AVELAPEGD"
     gene            5225..6193
                     /locus_tag="VIG33_06145"
     CDS             5225..6193
                     /locus_tag="VIG33_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY8270452.1"
                     /translation="MNSRKKAVFIRLDKIGDLICTLPMDQAPELKDWEITWVIAKGLS
                     FIPEHAEPKRRYIELDKNNPPEARQKFAELLKEFKPQVAISIQAPWWIGFLLWKNKVA
                     KRIGVLSKWDSFLFLNASLRQKRSQAVKHEAEYNFDLVRQIFPSQNLSAKAPVLKLKA
                     KPQPRCLEQYFLEPKKYTVVHPGMAGSALNWPILHYIRLIENLTARFKVVLTGTPADE
                     PWLKEIKEHFKNNSQVICLQNRLDTSQLLYILEQAAAVFAPSTGVIHMAASLGTPVFG
                     FYSPILVQSETRWGARGPGPIHLFTPPKENPSMSSITPEQVLGALQ"
     gene            6193..7203
                     /locus_tag="VIG33_06150"
     CDS             6193..7203
                     /locus_tag="VIG33_06150"
                     /inference="COORDINATES: protein motif:HMM:NF020804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit Rsm22 family protein"
                     /protein_id="HEY8270453.1"
                     /translation="MQRIFTFPPQLAHKIEKILRVQFQIGLQDSKKIAEAVKKLSDYF
                     IQNPLGSTPWNESWAQIAYLCYFLPLNHARAQAVVKEAEFQKFFEGLTEVIDFGSGPG
                     TASLALTTYFNKFTMIEKAKEICSRLEFLPAQQTSWAAVAPTFQEPQKTLAVFSYSLT
                     ELRSLPTWTKQIEALMIIEPATQEDGRKLLELRQELVGAGYSIWAPCTHQGPCPLLTQ
                     SKNDWCHDRIHFDMPKWLEEVESYLPMKNRTLTMSYLLARRRNAPPRNANLGRSVGDY
                     LKEKGKDRQMFCRSSEREFLTWMHKTGLHQETSRGVLVEIPEDTPKVSNELRLKSSVR
                     TF"
     gene            complement(7229..7999)
                     /locus_tag="VIG33_06155"
     CDS             complement(7229..7999)
                     /locus_tag="VIG33_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HEY8270454.1"
                     /translation="MNQVNPICANDFRTFLEQELARRNSANPNYSLRAFARDLSVDSS
                     FLSKLLNGKRSMTVRTIMSLAPHLALSQDQAKAFSQRATSRRRRYSLSELEVPVFQEI
                     ENQRFIQMMEWYHAALLELVSVSGFEANGAWIGERLQITAEQSQQALEDLISAGVLSQ
                     GEDGVWKSEIAHHTVSAKKFPRVQLLKKQIYEQALAALPHYEGTHGAITVAISEKRYE
                     EAVERIARFRRELSQFLSEPDEKDRVYQILISLFPISK"
     gene            8202..9299
                     /gene="gcvT"
                     /locus_tag="VIG33_06160"
     CDS             8202..9299
                     /gene="gcvT"
                     /locus_tag="VIG33_06160"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY8270455.1"
                     /translation="MDTPLLQKTPLAEEHVKLGARMVDFAGWWMPVQYKGLREEHDNV
                     RTNVGLFDVSHMGEVRFKGPKALETLQWLTTNDVAKLNENEAHYSLLPNAQGGLVDDI
                     IVYCLTKNEDYLVCVNASNADKDFAWMTKNNKGADITNESSYWAQIAIQGPKALALCD
                     KIFGFKASDMKSFTLRRFDYKGHKAILATTGYTGEKGGEVFIETKGAVTLWQDLLAQG
                     QEFAVQPIGLGARDTLRTEYKMSLYGHEIDDTTNPYEASLGWAVKPAAKDFMGKAPML
                     KIKEEGLKKQLIGFKMLEKGIPRQGYSLFSFDNKEIGKVTSGTHSPTLDEPIGIAYIS
                     SEYAAQGTEFSVDIRGRKVKAKVCKTPFVTK"
     gene            9309..9695
                     /gene="gcvH"
                     /locus_tag="VIG33_06165"
     CDS             9309..9695
                     /gene="gcvH"
                     /locus_tag="VIG33_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY8270456.1"
                     /translation="MVFHIPEELYYTKEHEWAQIDENIVTVGITEFAQEQLGEIVYVE
                     LPEEGQKVTQGQTFGVVESVKAASDLYSPVSGTVIEINAGLNDDPSVLNDDPMNDGWL
                     IRIEMDTEKELANLMRAPDYKKLVQA"
     gene            9704..9786
                     /locus_tag="VIG33_06170"
     tRNA            9704..9786
                     /locus_tag="VIG33_06170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9738..9740,aa:Leu,seq:tag)
     gene            9918..10496
                     /locus_tag="VIG33_06175"
     CDS             9918..10496
                     /locus_tag="VIG33_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270457.1"
                     /translation="MNSAEAIFNFLENGQISPEENFYYCQTRDRASSRLPKWMSYFQN
                     LGLSEHRSAILTAIIGELTNNSFDHNLGKWKEPAGCAVAFEKQDDLLNIYIADRGQGI
                     ISSLGSSLTATLPPDIILKKAFEERISGRAPERRGNGLKFVLKHLQESNNSLYCVSQG
                     YIYSIGPKPKSISEEHIQRIFSTLVVIQWSIK"
     gene            10493..10765
                     /locus_tag="VIG33_06180"
     CDS             10493..10765
                     /locus_tag="VIG33_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270458.1"
                     /translation="MKLNIKTFGDFLTSRPAGREAALTSLAYNSGLKGAKNVSLDFSG
                     VIVMTPSWLSEFVQTLKEKGIEKIEFLPSENPTVISSIEFIEPEIE"
     gene            complement(10821..11312)
                     /locus_tag="VIG33_06185"
     CDS             complement(10821..11312)
                     /locus_tag="VIG33_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270459.1"
                     /translation="MKTLKVNNLILFLSLWILGCATSSYKIVTNSENKKEIITTSDRI
                     ALECSDVGSDEGAFFGFSIHVLDNENTVIDVIQTNKLDKESCEKRIAKISNILGRGQL
                     IHIYGLGSLEEPREQEKAVYLFKGLGTYHGNGRVLQFMYILNEKGGCYDAYSADKKPC
                     PQD"
     gene            complement(11493..11936)
                     /locus_tag="VIG33_06190"
     CDS             complement(11493..11936)
                     /locus_tag="VIG33_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270460.1"
                     /translation="MNMKKIIVMVSVVVLSSTAAFAKLSKANFVRKCASLVTDNGRVG
                     PDFSCAAKTDADAKKLKSIGVKPQFSITIDESAEVGTSYSGSNKLFADVAVTVKSGLF
                     GIGSKSLRLACQETPAGGVEVYSPQILVDIQDNIDSKVTAEVTCR"
     gene            12058..13440
                     /locus_tag="VIG33_06195"
     CDS             12058..13440
                     /locus_tag="VIG33_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEY8270461.1"
                     /translation="MKDKSQELQRTLGLLPSASLVVGTIIGTGVFLKAAIMSQLVGSS
                     FWVLVAWAVAGVLSLAGALTYAEIGILFPRAGGEYVYLREAYGNLTAFLYGWQRFLIS
                     NTGTIAAYAVGAATFAAPLFDLSWCGGVKGFSILLIAIFSGLNCLNVTFGGRLQSVMT
                     IIKVMMIFFIVIGILFLSPTSSFSYISAGGGAFPGFSAFGSAILAALWAYDGWNNLPM
                     VAGEIKNPSRNIPLSLGLGVFFIVAIYVLIHFCYFMAMPFSEVISANSNIHPDALPVA
                     AKAAQSFLGSTGVWFLGIAMIFSAVGAMNGSILTSARIPYAMAIDGLFHRSLATLHKK
                     SKSPYVSVLTQGMIAAVLALSGTFDQLTDYVVFASWIFYALVASSVFVFRRKHPEMAR
                     PYKTLGYPYLPVLFLISALLLLINTVWTMPKESSIGLALILLGVPFYYFMERAPVSSS
                     PVVSNELPKP"
     gene            13565..>14083
                     /locus_tag="VIG33_06200"
     CDS             13565..>14083
                     /locus_tag="VIG33_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270462.1"
                     /translation="MMKLTNEQIVAEVMKSPNPKVKVAITDIDGVLRGKYLHKDKFLS
                     AVNGGFGFCNVVFGWDISDLAYEGMDFTGWHTGYPDAIASLDLHTFRKVPWDHDVPFF
                     LGDFDLDVCPRNLLKKVKKDSIDSGYKPFFGQEFEWFNFRESCESFQAKNYHDPQPLT
                     PGMFGYSLIRLSK"
BASE COUNT         3856 a         3353 c         3074 g         3800 t
ORIGIN      
        1 tcattgaaac tttcgtctca ttctaccaat caagaattcc tgggtcgcca tattggacct
       61 cgtgaagaag acattcaaac aatgctaaag gagttgggtt tctcttctct tgaagaaatg
      121 gtcgcccaag ttattccgca aaaaattcgc accactaaaa aatatcctgt ggtaggggat
      181 ggagtttctg aatacgaact gctggggaat ctgaaaaaaa ctctctctaa aaataaagtt
      241 tatcgctctc tgataggtat gggtttctac gacaccatca cgccttccgt gattcaaaga
      301 aatattctag agaatcctgt atggtatacg gcctatactc cttaccaagc ggagattgcg
      361 caaggtcgtt tggaagctct gctgaacttc caaaccatgg tgatagatct tacgggtatg
      421 gagatctcta atagctcttt gctggacgaa ggcacggccg ctgccgaagc tatggcgatg
      481 gctcacgctt ctcacaagca aagtgatgtc acccacttct tggtctctcc gaaaatgcat
      541 ccccatgtga ttgaagtttt gaaaacacgg tctgagcctc tggggcttac tatgctgatt
      601 caaaatccaa aaatttatga tttcaaaacc ccggtctttg cggccttcgt tcaatacccg
      661 gacactgagg gcatgattga agactacaaa gagatgacgc aaaaattcca tgaccatcag
      721 gccctggtgg tggcggctgt ggatctgctg tccttgactc ttttgacacc tccaggagaa
      781 tggggcgctg acatcgtggt cgggaattct caacgtctgg gtgttccgct cggattcggc
      841 ggacctcacg cggctttctt ggccaccaaa gatgcctata agcgtatcat gccaggccgt
      901 attgtgggag tctctgtgga cagccatggc aaaccagctc tgcgcttggc tttgcaaact
      961 cgtgaacaac atatccgccg cgaaaaagcg acttccaata tctgtacagc tcaagttttg
     1021 ctggccaata tggcgagcat gtatgcggtt taccatggac ctgaaggtct taaaaaaatc
     1081 gctcaacgaa tccatcgcct gaccgcggtg acggctgaag gcttgaagaa attaggcttt
     1141 gctattccga ccaccccatt cttcgatacg ctcactgtga ctactttaaa agccgcggaa
     1201 atagtcaaag cagcggaagc cctgcaaatg aacttgcgag tgattgatca aaaaactctc
     1261 ggtttgtctt tagatgaaac ttgcaactta caaacagtaa atgacatttt ccaagctttc
     1321 aatttaggtc taaaagcccc atttacggct actgagatcg ataaggattt gaattactct
     1381 tggccgacag catttatcag aaccagcaaa tatctcactc accctgtgtt taatacccat
     1441 cgttctgagg ctgagatcac tcgctacatt catatgttac aaaataaaga catcactttg
     1501 acccactcga tgattccgct tggatcatgc accatgaaat tgaatgcaac cacagagttg
     1561 cttcccgtct cctggcctga ggtcaacaaa atgcatcctt ttgctccgac ttcacaaatt
     1621 caaggctatg ttgaaatgat tcatgacctt gaaaataaac tttgcgatat cacgggtttt
     1681 gcaggcatta gtttgcaacc gaactcaggc gcccaaggcg agtacgcagg gcttctggtg
     1741 attcgtaagt atttcatcga taagggcgaa gctcacagaa atatctgttt gatccctagc
     1801 tcggctcatg gaaccaatcc cgcttctgcg agcatggcgg gcatgaaggt cgtcgtcgta
     1861 aactgcgaca agcaaggaaa cgtcgaagtt aatgacctca aaaccaaagc ccgagaacat
     1921 aaagacaatc tggcggcttt gatgatcact tacccctcca ctcatggagt gtttgaggaa
     1981 agtatcaagg agatctgcca aatcattcat gataatggcg gtcaagttta tatggacggc
     2041 gcgaatatga atgccctcgt aggcctttgc cgtcctgcgg agtttggggc ggacgtttcg
     2101 catatgaatt tacataaaac attcgccatc cctcatggag gtggcggtcc cggcattggc
     2161 cctatcggtg tcggcgaaca tttgaagaaa tatttaccta ctcatagctt gcaacctgaa
     2221 gcgggtccta agacaggaat cagcgccacg acttcggctc cgtggggaag cgctagcatt
     2281 cttccgatct cttggagtta catcacaatg atgggagccg aaggtttaaa gaaagccacg
     2341 ctggtcgcaa ttctgaatgc gaactacatc gcgaagaaac tcgctcctca ttaccctatt
     2401 gtgtacaagg gcaaagaagg catggtcgct catgaatgta ttctggatct tcgcgatatc
     2461 aaaaaagtca gcggcattga cgtcagcgac gtcgcaaaac gtttgatgga ctatggcttc
     2521 catgccccta ccatgtcatg gccggtggtg ggaacgctga tggtggaacc gacagagagt
     2581 gagccaaaag ctgaactcga tcgtttcatc gaggctatgg tggcgattcg caaggagatc
     2641 accgccgtgg agagcggcaa aatggacctt aaagacaatc cgttggtcaa ttcaccccac
     2701 actgttgaca gcttgctaaa ggctgagtgg aaccatgcct attctcgaga acacgcggct
     2761 tatccagttt catggttgcg ccagaataaa ctctggcctg ctgttggacg cattgacaat
     2821 gtgtacggcg atagaaactt gatctgcgct tgcccaagta ttgagactta taagtaaatg
     2881 agcagatcca ctgcaaggat aaaaatagta atggtaatgg taatggcatt ggcttcggcc
     2941 tttgccattt ttattcctca gagtcttttt gcaagcgttc cctcgcattt tctttggagc
     3001 gactccaaag gcctgatgac ggtcgaggcc acttgtcaac tgagacacga agaacagaat
     3061 ccgtttttca tttcgcaaaa acctaactct caactcaatg actactcaaa attgaagggc
     3121 ttaggcttct tcggaggatg ggtttcggga aagctcttgg atcgctcgct gattacagcc
     3181 aaagaagccc ccaacgcggc ggatgactct caaatccgcg tactgagcgt cgctaagaat
     3241 gccactcgtc cggacgttct ccatatttct gaagcgggcg atgaaggtta tcttgccaaa
     3301 gaatctcttc aagatatcgg caacttcgtt attgaaatta aagacgctcc ggcattttta
     3361 aatttaaaat cacccttcag aattcaaaaa agcttttggc aagccgctac tgaggacgaa
     3421 aaattccttg ccttcacttg cggagatatc gcagcacctg tgcggtattt ccttttcgaa
     3481 gtctatgatc tggcccattt ggaccccgtc gcccaagtgg gagtgaatct ggctgagacc
     3541 aaaatctggg attcaatcaa tgtctacact ccagaggaag ccaatcaggt ggtcaaagag
     3601 gaagcggaac acactccccc cgcccctcag ccgccgacag aagttcccct tccctcccca
     3661 cagcctccct ctgagggcca agaagacacc cccattttgg aaggggagct agaatatgtc
     3721 atctgtaccg aagataaaac cacaggcatt ttcgctcagg atttaaaaac taaaattttc
     3781 tccgccaatc aattcgaaac agttattcct agtcaatcct gggatgaaaa acgaaagacc
     3841 acacacgtgg aagtccaatt tccggctcgc gaagaaatgc cccgctccgg ttggattcct
     3901 cgagatctcg tgcaactgag agctgagtgc attccgctta aaaaaaccgc cccgagtgat
     3961 gaagatgctc ccgtggatga ttcactcacc actctcgatc cccgtgtttc gagaaaagat
     4021 tgctgtaaat ttccaacgac tcaaagatct tcccagcctt ataccagtgg aattcttcaa
     4081 ttcagggcga aacgcaataa aggcaaacgt cgacacgctg gctgtgacct ttatcgctct
     4141 aagggggagg ccgcagtggc cgtagcctca ggcaccgtcc tccgcggatt gtattacttc
     4201 tatcaagggg tgtttgccat cgaggtgaaa catccaaaat ttattgctcg ttatggcgaa
     4261 gtccttggca aatatggccc gggagtggct aaaggcaaaa atgtcgtggc cggtcaagag
     4321 ttgggctata ttggcaaagt gaactcaggt tgttgcgctc ccatgcttca ttttgaaatg
     4381 tataaaggaa gcgctcacgg ccctttaacc cgccaccgtg tgcctcctta cgaccgacgc
     4441 tctgacgtca tgaatccaac agatttttta aaacaatggg aaaaagtcca attcggacag
     4501 tcttactaaa tgaataagag gttcctttta atcatctttg gctcactctt gctgatgagt
     4561 ggaattttct tcactgcctt ctcttcgcga aagcctttga ccccggaaac tcaagtctca
     4621 gtgaagtctc ccgtctttca cgaggagtct ccctcaaccc ctcctagtaa agacctgtct
     4681 cctgccaaag ccaatgccgc ctcattttcc ctgaaggaaa tgagcaagat tctttttcaa
     4741 cacacggtga ccggaggtaa actcgaagat ttggtcaagg atcttaaaaa gacccggcaa
     4801 gagccctttc tagttcgcga taaaaatgac gagaccggcg aaatgatcat tgtgcgcacg
     4861 aagagtccgc tgccgggcac ccgttatttc catgcccagt attttaccga tgaaaccatg
     4921 caagcctttg cgcagcacat gtcttttgaa cttcatgcca gtccgcaggc gatgaaccag
     4981 gcgattcaaa ccgtcaaaga agctttcccg aatctggatg ctcctagcga agaaagccgt
     5041 gacttcatcc aatgggatct cgaggatggt tatgttattt ggatcaaacg tttgggagct
     5101 gaagatatcc aggaaaatcc ttttaatttt tattccgctg aagacatcgg cagcattcgc
     5161 atcgccgtgg aactcgctcc tgaaggcgat tgatacgcga ctcaagttca ggtattcttt
     5221 ccctatgaat tcaagaaaaa aagccgtttt tatacgtctc gacaaaattg gcgacttgat
     5281 ctgcacgctg cccatggatc aagctcctga gttaaaggat tgggaaatca cttgggtgat
     5341 tgccaaaggc ctttcattta tacctgagca cgcagaacca aaacgccgtt atattgagct
     5401 agataaaaac aatcccccag aagctcggca aaaattcgca gaactcctca aagaattcaa
     5461 accccaggtg gcgattagca tccaagctcc ttggtggatc ggttttcttt tatggaaaaa
     5521 taaagttgca aaacgcattg gtgttttatc gaagtgggat agttttttgt ttttgaatgc
     5581 cagccttcgc caaaaacgca gccaagcggt aaaacatgag gcggagtata actttgattt
     5641 agtgagacaa atcttcccga gtcaaaatct ttccgcaaag gctccggttt taaaactcaa
     5701 agccaaacct caaccccgtt gtttagagca gtactttctc gagccgaaga aatacacggt
     5761 ggtacatccg ggcatggcgg gctcagccct gaattggcct attcttcatt acattcgtct
     5821 cattgaaaac ctcacggccc gattcaaggt ggttttaacc ggaacacctg cggatgagcc
     5881 ctggctaaaa gaaatcaaag agcattttaa aaataactct caagtgattt gtttacaaaa
     5941 cagactcgac acctctcagt tactttacat tttggagcag gcggcagcgg tctttgctcc
     6001 gagcaccggg gtgatccata tggcggcaag cctaggcact ccagtctttg gtttttactc
     6061 tcccattttg gtgcaatctg aaacacgctg gggagcacga ggccctggtc ccattcacct
     6121 cttcacccct cccaaggaaa acccttcaat gagtagcatt acccccgaac aagttcttgg
     6181 agcacttcag taatgcaaag aatttttact tttcccccac aactagcgca caaaattgaa
     6241 aagattcttc gcgttcagtt tcagatcggt ctgcaagact ccaaaaaaat cgccgaggca
     6301 gttaaaaaat tgtcggacta ttttattcag aatcccctcg gcagcactcc ttggaatgag
     6361 tcctgggcac aaatcgctta cctctgttat tttcttccac tcaatcacgc ccgggctcaa
     6421 gcggtcgtga aagaggctga atttcagaaa ttttttgaag gactgacaga agttattgat
     6481 tttggctcag gtcccggcac cgcaagtctt gcgctgacga cctattttaa caagttcacg
     6541 atgattgaaa aggccaaaga aatttgttca cgcttggagt ttttgccagc tcaacaaacc
     6601 tcttgggctg cggtggcccc cacttttcaa gagcctcaga aaaccctggc agttttttct
     6661 tattcactga ccgagcttag aagtcttccg acatggacca agcaaataga agctttgatg
     6721 attatcgaac ccgcgaccca agaagatggc cgtaagctcc tagagcttcg ccaggagctg
     6781 gttggggctg gatacagtat ttgggcccct tgcacacatc aaggtccatg tcccctgctg
     6841 actcaatcaa aaaatgattg gtgccacgac cgcattcact ttgacatgcc caagtggctg
     6901 gaagaggttg aatcctattt gccaatgaaa aatagaacac tgactatgag ctacttactg
     6961 gctcgccgaa ggaacgctcc ccccagaaac gccaacctag gccgtagcgt tggagattat
     7021 ctcaaagaaa aaggcaagga ccgccaaatg ttttgccgaa gttcagaacg tgaattcctg
     7081 acctggatgc ataaaacggg attgcaccaa gaaacctctc gcggagtact ggtggaaatc
     7141 cccgaagata ccccaaaagt ttccaatgaa ttgaggctaa aaagttcggt ccggactttt
     7201 taggtttagt gaattcatta tttagcagtc atttagagat aggaaacaaa gaaatcaaga
     7261 tttgataaac cctgtccttt tcatctggct cagataaaaa ctgactgagc tcacggcgaa
     7321 atcttgcaat acgttccacg gcctcttcat aacgcttttc agagatagcc acagtgatgg
     7381 ctccatgggt gccctcatag tgaggtaaag cggcaagtgc ttgctcataa atttgctttt
     7441 ttagaagttg tacgcgcgga aattttttag ccgacacggt atgatgagct atttcactct
     7501 tccaaacgcc gtcttctcct tgggaaagaa ctcctgcact gatcaaatcc tcgagagctt
     7561 gctgggattg ttccgcggtg atttgcagtc gctccccaat ccaagcgcca ttggcttcaa
     7621 agccgctcac actgactaac tctaagagag ccgcatgata ccattccatc atttgaataa
     7681 atctttgatt ctcaatctct tgaaagaccg gaacctcaag ttcacttaat gaataacgac
     7741 ggcgacgact ggtagccctt tggctgaagg ctttggcttg atcttgcgac aaagccagat
     7801 gcggagctaa actcataatg gttcgcacgg tcatcgacct tttgccatta agaagtttcg
     7861 ataaaaaaga cgagtccaca ctcaaatctc ttgcgaaagc acgcagtgag taattgggat
     7921 ttgcggaatt tctgcgagcg agctcttgtt ctaggaaggt cctaaaatca ttagcacaaa
     7981 taggattcac ctggttcata taccacacta atcaacttga aatttcccgc agagacataa
     8041 tgcgtaaaat aacgtgagca cgaatgcaag agaaaacaag gtctttactc aaacgcttgc
     8101 gtccacagta cctctgagaa acgtccacat ataatcatgg gagggttgac aagatctaag
     8161 agcccggcga cattgccaaa tatcgaaatt tggaggctct tatggataca ccccttcttc
     8221 aaaaaacccc tctggcagaa gaacacgtaa agctcggcgc ccgcatggtg gatttcgctg
     8281 gctggtggat gccagttcag tacaaaggct tgcgcgaaga gcatgataac gttcgcacca
     8341 atgtcggact ttttgacgtc tctcacatgg gagaagtgcg ttttaaaggc cctaaggccc
     8401 tcgaaaccct gcaatggctt accactaatg atgtggcaaa attgaacgaa aatgaggcgc
     8461 actacagcct tcttcccaac gcacagggag gcttggttga tgacatcatc gtttattgcc
     8521 tgactaaaaa cgaagactac ctagtttgcg tcaatgcctc aaacgccgac aaagactttg
     8581 cctggatgac aaagaacaac aaaggcgctg acatcaccaa tgagtcttct tactgggccc
     8641 aaatcgccat tcaaggacca aaagccctcg ctctttgcga caaaatattc ggctttaaag
     8701 ccagcgatat gaaatccttc acgctgcgac gctttgatta caagggccat aaagcgatct
     8761 tagccaccac tggttatact ggcgaaaaag gcggtgaagt ctttatcgaa accaagggag
     8821 ccgttacact ttggcaggat ctactcgctc aaggacaaga atttgcagta caacccattg
     8881 gcttgggtgc ccgtgatact ctccgcacgg aatacaaaat gtcgctttac ggtcacgaaa
     8941 tcgatgacac aacgaatcca tacgaggcga gcctcggttg ggctgtgaaa cccgcagcga
     9001 aggactttat gggcaaagcc cccatgctta aaatcaaaga agaaggttta aaaaagcagc
     9061 tgattggttt caaaatgctc gagaaaggga tccctcgcca aggctattcc ctattttctt
     9121 ttgacaacaa agaaatcggc aaggtaacta gtgggactca ctcgccaacc ctggacgaac
     9181 ccatcgggat cgcatatatt tcttcggaat atgcggctca aggaactgaa ttttcagttg
     9241 atatccgcgg acgtaaagtg aaagcgaaag tttgcaaaac acctttcgtg acaaaatagg
     9301 agaagctcat ggtctttcac atccctgaag aactttacta caccaaagaa cacgagtggg
     9361 ctcaaattga cgagaacatc gtcactgttg ggatcaccga gtttgcccaa gaacaattgg
     9421 gagagattgt ttacgttgaa cttccagaag aaggtcaaaa ggtaactcaa ggtcaaactt
     9481 tcggcgttgt cgaatctgta aaagcggcga gtgatcttta ctcgccagtt tctggaacag
     9541 taattgaaat taatgctgga ctgaatgacg atccttcagt attaaatgac gacccaatga
     9601 acgacgggtg gttaatccgt atcgaaatgg ataccgaaaa ggaacttgca aacctaatgc
     9661 gtgctcctga ttacaaaaag ctcgttcaag cataagaccg caagccccgc tggtgaaatt
     9721 ggcagacacg ctagacttag gatctagtgc gcaagcgtgg gggttcaagt ccctcgcggg
     9781 gcaccattat tcgggtacgc cgtacctttt tcggatgcag tgcaaaaaaa taagactgtc
     9841 atctttctta caacttcgca gctcaagatc tcagcgtttg atttgcttcc tctccgtttg
     9901 caggctcaaa tataatcatg aactcagcag aagctatttt taatttttta gaaaacggac
     9961 aaatttcccc tgaggaaaac ttctactatt gccaaacacg ggaccgcgct tcgtctcgcc
    10021 tgcccaaatg gatgagctat tttcaaaacc ttggtctttc cgagcatcgt tcagcaatcc
    10081 tcactgcgat cattggcgaa ttaaccaata attcttttga tcataatctc ggcaaatgga
    10141 aagaacctgc aggctgtgca gttgcttttg aaaagcaaga cgacctatta aatatatata
    10201 tcgcagatag aggacaggga attatttctt ctctgggaag ttccctcacg gcaactcttc
    10261 cccccgatat catattaaag aaagccttcg aagaacgaat ttccggcaga gctcctgaac
    10321 gacgtggaaa cggattaaag tttgttttga agcatcttca ggaatcaaat aactctctct
    10381 actgcgtgtc ccaaggatat atttactcca ttggcccaaa accaaaatcc atatcagaag
    10441 aacatataca aagaatattc agcactcttg tcgttattca atggagtata aagtgaaatt
    10501 aaacattaaa acattcggag attttttaac ttcaagacca gcggggaggg aagccgctct
    10561 cacttccctt gcttacaact ctggacttaa aggtgcaaaa aacgtaagcc ttgatttttc
    10621 tggagtcatc gttatgacgc catcgtggct cagtgaattt gtacaaactc taaaagaaaa
    10681 aggaatagaa aaaatcgaat tccttcctag cgaaaatccc acggttattt catcgattga
    10741 atttattgaa cctgaaattg agtaactcaa gagcctaaaa aatttgccgt gctgatctca
    10801 ggtaaatgga aagtttatac ctagtcttga gggcaaggct tcttatcggc tgagtaagca
    10861 tcgtagcaac cacctttttc attcaaaata tacatgaact gcaagactcg gccattccca
    10921 tgatacgtcc ctaacccttt gaagaggtag acagccttct cttgttctcg tggctcctca
    10981 aggcttccca aaccataaat atggatgagc tgtcctcgac ctaaaatatt actgattttt
    11041 gcaattcgct tctcacaact ttccttatcc aatttattag tctggataac gtctatgacc
    11101 gtattctcat tatctaagac atgaattgaa aaaccaaaaa aagctccttc atcagacccc
    11161 acatcagaac actctaaagc gatccggtcc gaggttgtga ttatctcctt tttgttttca
    11221 ctgttggtta caatcttata agaggaagtt gcacacccca aaatccatag ggacaaaaat
    11281 aggatcagat tatttacttt taaggttttc ataagactct ttatattttt tcagtgcttt
    11341 cttttttcac tctgttttac tttttagaat tcccttatat tccaaaatag tttcttcgtc
    11401 agtaggacta cggtcaagtt tactggcagc tctttgcctt ttccacgcaa gccaccgaat
    11461 agccccactt atcgtggcta ttcttcatga aactatcggc aagtcacttc agcagtgact
    11521 ttagagtcaa tattatcttg aatatctaca agaatctgag ggctgtagac ttcaactccc
    11581 ccagcaggag tttcttgaca agcaagacgg agggatttcg aaccaatgcc aaatagacca
    11641 gatttgactg tcactgcaac atctgcgaaa agcttgttac ttccgctgta gctagtacca
    11701 acctcagcag actcgtctat cgtaattgaa aattgtggct ttactccaat ggattttaat
    11761 ttctttgcat cagcatccgt ctttgccgca caagaaaaat ctggtccgac acggccatta
    11821 tctgtcacta aagatgcaca ttttcgaaca aaattcgcct tgcttaattt tgcgaaagcc
    11881 gcagtggaac tgaggacaac cactgacacc ataacgatta tttttttcat gttcataatt
    11941 ttctcctatt aaggtaaata aagataaatt tgaaagacag tatcataggt caaataaatg
    12001 acttaacttt aaatatttag acaggtaaag ttttcaaatc ccataaaagg aatttgtatg
    12061 aaagataaat cacaggaatt gcaacgcacc cttggcctcc taccttccgc ctccctcgtc
    12121 gtgggcacaa ttattggaac cggagtcttt ttgaaagcgg cgataatgtc ccagcttgtt
    12181 ggttcttcct tttgggttct ggttgcctgg gccgtggccg gagttctttc tcttgccgga
    12241 gctctgactt atgctgaaat tggaattctt tttccacgcg cgggtggtga atatgtttat
    12301 ctccgtgaag cctacggcaa tttgacggct ttcttatatg gctggcagcg cttcttgatt
    12361 agtaatactg ggacgattgc cgcctatgcg gtcggagccg ccacttttgc cgctcccctt
    12421 tttgatctga gctggtgcgg aggagttaaa ggattttcca ttcttttaat cgcgattttt
    12481 tctggattaa attgtttgaa tgtcactttc ggcggacgtc ttcaaagcgt catgacaatt
    12541 ataaaagtga tgatgatttt ctttattgtc attggaattt tgttcctttc tccgacctct
    12601 tcattttctt atatttccgc agggggaggc gccttccccg gtttcagcgc ttttggctcc
    12661 gcgattctgg cggcactctg ggcctatgat ggctggaaca atctccccat ggtggccggg
    12721 gaaattaaaa atccttcgcg caatattcct ctttctttgg gtttaggtgt tttctttatc
    12781 gtggcgattt atgtcctgat tcatttttgc tattttatgg cgatgccttt ttcagaagtg
    12841 atctctgcca attcaaatat ccatcctgac gcccttcccg tcgccgcaaa agccgcccag
    12901 agctttttag gctccacagg tgtgtggttt ctaggaattg cgatgatttt ctcagccgtt
    12961 ggagccatga atggctctat tttaaccagt gcacggatac cctatgcgat ggccattgat
    13021 gggctttttc atagatcatt ggccactctc cataaaaagt cgaagtcccc ttatgtctcc
    13081 gtgctgactc agggcatgat cgccgcggtt ttggcgcttt ctggaacctt tgaccaatta
    13141 acggactatg tcgtttttgc ttcttggatt ttttatgccc tcgtggcctc ttcggttttc
    13201 gtttttcgtc gcaagcatcc tgagatggct cgtccttaca agacgttggg ttatccttat
    13261 cttccagtgc tgtttttgat ttcagccctc cttctcctga ttaatacagt ttggacaatg
    13321 cctaaagaat catcaattgg tttagcattg attcttctgg gagtgccttt ctattacttt
    13381 atggaaagag ctccggtatc gagcagtcca gttgtcagca atgagttacc gaaaccctaa
    13441 tctccatgac ctagcccgat atagattcta agtttccaga ggctccaccg actttttatt
    13501 gccagtgggc ggtggccttt gtaaaactgt ggctgaaaat atttatagga taacgggagc
    13561 tgggatgatg aaattgacca acgaacaaat tgtcgctgaa gtaatgaagt ctccaaatcc
    13621 taaagtcaaa gtggccatca ccgacatcga cggtgttttg cgcggaaaat atttacacaa
    13681 agataaattc ctttcagcgg tcaacggcgg ctttggtttt tgcaatgtgg tttttggttg
    13741 ggatataagc gatctcgctt atgaaggaat ggattttacg ggatggcata cgggataccc
    13801 tgacgccatc gccagcttag atcttcacac ttttcgtaaa gttccctggg atcatgatgt
    13861 gccctttttc ctaggagatt ttgatttaga tgtctgtcct cgcaatctct taaagaaagt
    13921 aaaaaaagac tctatcgatt ctggctataa acctttcttt ggccaagagt ttgaatggtt
    13981 taactttcgt gaaagctgtg aatcctttca agccaagaat tatcacgacc cccaaccgct
    14041 gactccggga atgtttggtt attcgctcat tcgtctttct aaa
//
LOCUS       DATBTE010000164         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2412339, whole genome shotgun sequence.
ACCESSION   DATBTE010000164 DATBTE010000000
VERSION     DATBTE010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2412339"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="VIG33_06205"
     CDS             <1..1042
                     /locus_tag="VIG33_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8270463.1"
                     /translation="DYLHIRKKIHSRVEIMGPVSELIATLLILGVLLYISTEIGSGRA
                     SMGSFITYVSSLIGLNMPLKKFQESYVRIQEVIVSAQRVYSIVDNPNIVSESGKNLPF
                     PKAWTLIEYKDVSFAYGKELILKNINLTIRRGERLALVGESGSGKSTIVNLLERFFDA
                     SSGEILIDGINILDINLQDLRKNIALVNQDVFLFSDSIEKNIQAGDFERQLSDVPAMA
                     KHANAHDFIMRMPEGYQSLVGDRGNLLSGGEKQRISIARALFKNAPILILDEATSALD
                     TSSEIEVQKGLDYLMEGRTALIIAHRLSTIQTADKILVLKSGQIVEIGTHQQLLDSKG
                     EYHRFYSLQHRI"
     gene            1186..>2198
                     /locus_tag="VIG33_06210"
     CDS             1186..>2198
                     /locus_tag="VIG33_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY8270464.1"
                     /translation="MMINWEEFEHIHVIKKLKQILGAWWNIDVIFTDERGQLKGFDQE
                     KTQFNNPVIAQLMQKEAAKANISEMVVKTIDDLRTSQNRFSLRKWDMVGFDVGIFPIL
                     IENDFVGTVIATGFFRDQNNMPRLEEIRERMAAFGMSADTIDKCLGKLKYLEDQDRNH
                     FCEVCELVAQEVVTLHLEITSRENRIRELNKELGNRFKYDNMIGKSKPMQSLYSLLDK
                     IKGADSTVLIQGENGTGKELIAKSIHYNSHRKDKSFVIQNCSAFNDNLLESELFGHIR
                     GSFTGALKDKKGLFEMADKGTFFLDEIGDTSPTMQVKLLRVLQEGTFTPVGATEQRKV
                     DVR"
BASE COUNT          660 a          455 c          455 g          628 t
ORIGIN      
        1 agactatctc cacattcgca aaaaaatcca ctcacgtgtg gaaataatgg gaccggtctc
       61 tgaattgatc gctactctct taattttggg agttttgcta tatatcagca ctgaaatcgg
      121 cagcggccgc gccagcatgg ggtcttttat tacttatgta tcttccttga ttggcttgaa
      181 tatgcccctt aaaaagtttc aggaaagcta tgtgcgtatt caagaggtta tcgtttctgc
      241 gcaaagggtt tactcgattg tcgacaaccc aaatatcgtt tccgaaagtg gcaaaaattt
      301 gccatttccg aaggcttgga cactcattga gtataaggat gtctcctttg cttacggcaa
      361 agaattgatt cttaaaaata taaatctcac tattcgccgt ggtgaacgct tggctttggt
      421 tggtgaaagt ggcagtggta aatcgaccat cgtcaattta ttagaacgct tcttcgacgc
      481 gagctctgga gaaattctta ttgatggtat caatattctc gatatcaact tacaagatct
      541 tcgtaaaaat attgccttgg taaaccaaga cgtgttccta tttagcgact ctatcgaaaa
      601 gaatatccaa gcaggcgact tcgaacgtca gctcagtgat gttcccgcta tggcgaaaca
      661 cgccaatgcc catgatttta ttatgcgtat gcccgagggt tatcagagct tggtgggtga
      721 tcgtgggaac ctcttatccg gtggcgaaaa acagagaatc agcattgcca gagccttatt
      781 taaaaatgca cccattctta ttctcgatga ggccaccagc gctttagaca cttccagtga
      841 aatcgaagtt caaaaaggtc tagactatct tatggaaggc cgaacggcat tgatcattgc
      901 tcacagactt tccactatcc aaaccgccga taaaatcttg gttctcaaat cgggacaaat
      961 tgtggaaata ggaactcatc aacaacttct cgattctaaa ggtgaatatc accgtttcta
     1021 ttctcttcaa catcgtatct agcgctctga actttctctc tgaactttag tcttgttgga
     1081 gcattttcaa tgcttgcgtc taatttttgt ccgctgccct taagtttgtc caatcttttg
     1141 acgatatgaa agttgaaaga ttagacagtt taagaaggag cgttcatgat gatcaattgg
     1201 gaagaatttg aacatataca tgtgattaaa aaactcaaac aaattttggg agcttggtgg
     1261 aatatcgacg tcatctttac tgatgaacgt ggccaactca aaggcttcga tcaagagaaa
     1321 acccaattca ataatcccgt cattgcacaa ttgatgcaaa aggaagccgc taaggcgaat
     1381 atttctgaga tggttgtcaa gacaatcgac gaccttagaa cttcacagaa tcgtttctca
     1441 ttaagaaaat gggacatggt gggattcgat gtgggtatct tccctatcct tatcgaaaac
     1501 gatttcgttg gaactgtgat agccactggt ttcttccgtg atcaaaataa tatgccacgc
     1561 ctagaagaaa ttcgtgaacg catggcagcc tttggcatga gcgcggatac tattgataaa
     1621 tgtctaggca aattaaaata tctggaagac caagatagaa atcatttttg cgaagtgtgc
     1681 gaattggttg cgcaagaggt agtgaccctc catcttgaga tcacctcccg tgaaaacaga
     1741 atcagagaac tcaataaaga actgggcaat cgttttaaat acgacaatat gatcggtaaa
     1801 tctaaaccga tgcagtctct ttattccctc ctcgacaaaa ttaaaggtgc cgattcaacg
     1861 gtgttaattc aaggggaaaa tggtactggt aaagagctca ttgcaaaatc aatccattat
     1921 aattctcatc gaaaagataa atcttttgtg atccaaaact gttctgcgtt caatgacaac
     1981 ctgttggaat cagaactctt cggtcatata agaggctcat ttacaggtgc actcaaagac
     2041 aaaaaagggc tttttgaaat ggctgataaa ggcacctttt tccttgatga aattggcgac
     2101 acctctccaa ctatgcaggt aaaacttcta cgcgttctac aagagggcac tttcactccc
     2161 gtaggtgcca ctgaacaaag aaaagtcgac gtccgcat
//
LOCUS       DATBTE010000165         2891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1727834, whole genome shotgun sequence.
ACCESSION   DATBTE010000165 DATBTE010000000
VERSION     DATBTE010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727834"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VIG33_06215"
     CDS             complement(<1..850)
                     /locus_tag="VIG33_06215"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY8270465.1"
                     /translation="MLKYGFGTWRFFLAFLVAISHLYANMIHGPAAYAVWGFYVLSGY
                     LMTYVLTTKYSFTVKGLQEFAFNRFLRIFPGYWIVTILGVITILVLKDQVSLTTLNPQ
                     FQLPNNFNEWLFVVTLLPVFPNARLPVPVASALGIEVGAYILMPFLAASRSASWLVLI
                     ISFIIHAQFDFIGDTFAVRYATFLPCLFPFAAGSLICHYRLFLLKFAAPKLSTFVWLA
                     QCLIWLSRPYYPWTYGLYISVALSAWVTLSLSNIKTGKLDSHLGELSYPVYLLHTTAA
                     AWLLPSM"
     gene            1087..2061
                     /gene="gmd"
                     /locus_tag="VIG33_06220"
     CDS             1087..2061
                     /gene="gmd"
                     /locus_tag="VIG33_06220"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEY8270466.1"
                     /translation="MKRALITGITGQDGSYLAELLLSKGYKVYGLTRRSSTNITGRID
                     HILSQVELLPGDLLDQSSLQAAVEEAKPDEVYNLAAQSFVQTSWNQPVLTSEFTATGV
                     TRVLEAIRHVNPKVRFYQASSSEMFGKVQAVPQVETTEFYPRSPYGVAKLYGHWITIN
                     YRESYNMHATSGILFNHESPRRGIEFVTRKISYTVAMIRHGLQKELRLGNLEAKRDWG
                     FAGDYVEAMWLMLQQDRPADYVVSTNEAHTVKEFVELAFERADLDWKRYVVIDEKFMR
                     PAEVDLLIGDSTKAKREMGWEPKTSFKQLVHMMVDSDIKLVGSNLNLK"
     gene            2070..>2891
                     /locus_tag="VIG33_06225"
     CDS             2070..>2891
                     /locus_tag="VIG33_06225"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8270467.1"
                     /translation="MVVWVTGADGFIGSALVRNLAHSGIRTLMPSLDIRDTAAIQKVV
                     SENSFDAVIHLAGMSSVKECEINKSLAFSVNSEAVYHLGEAIAKIKPQAQLIFPSTGH
                     VYQSYPMGTQEEIYTEKSSVLPRNFYALTKLQAEENLKFLAKNRGLKATVLRLFNHTH
                     KSQTQDFFLPSVYGQLLSAKKRGEKKALLTVGDIDVRRDIGAIQDLALAFQTVLFENP
                     YKSDEVKTFNISSGHAVGLRDLVNQLADKMEMEVDFKVDPQLIRENEPKSVVGINS"
BASE COUNT          885 a          522 c          715 g          769 t
ORIGIN      
        1 ccatgctggg gagtaaccat gcggcagcgg tcgtgtggag gagataaaca ggataagaaa
       61 gttcgcccaa atgactgtct aactttccag tttttatatt gctaagggaa agagtgaccc
      121 aagccgacaa ggccacggag atataaagcc cataggtcca tggatagtaa gggcgactca
      181 gccatatgag gcattgtgcc agccaaacaa acgtgctgag ttttggcgcc gcaaacttta
      241 aaagaaagag tctatagtgg caaattaaag agccggcagc aaaagggaaa agacagggaa
      301 gaaaggtcgc atatctgact gcaaaagtgt caccgatgaa gtcaaattgg gcatggatga
      361 taaacgagat aatcaaaacc agccaggaag ccgaacgact cgctgctaga aaaggcatta
      421 gaatataggc cccgacttca attcctaatg cactcgcaac gggaacggga agtctggcat
      481 ttggaaaaac agggagaagt gttacgacaa ataaccactc attgaaatta ttaggaagct
      541 gaaattgagg gtttagagtt gttaaactca cttgatcttt aagaactaga atcgttatca
      601 cgccgagaat tgtaacaatc caataccctg gaaaaatgcg gaggaatcga ttgaaggcga
      661 attcctggag tccttttacc gtaaagctgt acttcgtagt tagaacataa gtcatgaggt
      721 agccactcaa tacataaaaa ccccaaaccg cataagccgc tggtccatgg atcatatttg
      781 cgtaaagatg cgaaatagcg actagaaatg ccaagaaaaa acgccaagtt ccaaagccat
      841 atttaagcaa aacaactcct attcaatgaa gatcaaagga aaagtcgagg atagctaagt
      901 taatttttta tcttttcgtg tggttttctg tcaaagaaga aataatggtt ggagtagcaa
      961 tgatgacgca gtaatgttgg ctcttgggag atctaaaata ttaaaaagta aattttaaag
     1021 agaatcattg aaaatctact ttgaagcgtt ttaaatattt ttattattta taaattaagg
     1081 atttttatga aaagagcgtt gattacaggt attacggggc aagatggctc ctatttagcg
     1141 gaacttcttt tgtcaaaggg ctacaaagtt tacggactca cgcggcgttc gagcaccaat
     1201 attactgggc gaattgacca tatcctctct caagtggagc ttttaccagg ggatttgtta
     1261 gatcagagtt ccttgcaggc ggcggtcgag gaagcaaagc ctgatgaagt ttacaatctt
     1321 gcggctcaaa gctttgttca gacatcctgg aaccaaccgg ttcttacctc ggaatttacg
     1381 gccacaggcg tgacaagagt tcttgaggcc attcggcacg tcaatccgaa agtgcgtttc
     1441 tatcaagcct cttccagtga aatgtttggc aaggttcaag ctgttcccca agtggagacc
     1501 acggagtttt atccgagatc tccttatgga gttgctaagc tttacgggca ctggattaca
     1561 attaattata gagaatctta caatatgcac gcaaccagcg gtattctgtt taatcatgag
     1621 tctcctcgaa gaggaatcga gtttgtaacc cgtaaaatta gttatacggt tgcgatgatt
     1681 cggcatggtc tgcaaaagga actgcgattg gggaacttgg aggcaaagcg tgattggggt
     1741 ttcgctggag attatgtaga agcgatgtgg ttaatgcttc agcaggacag gccggcagac
     1801 tatgtagttt caacgaacga agctcatacc gtcaaagaat ttgtggaatt ggcttttgag
     1861 agagcggatc ttgattggaa gcggtatgtg gtgattgatg agaaatttat gcggcctgca
     1921 gaggtggatt tacttattgg cgactctaca aaagccaagc gagagatggg atgggagcct
     1981 aaaacctcat ttaaacaact tgttcacatg atggtggatt cagatattaa attggttggt
     2041 agcaatttga atttgaaata gagggtgcca tggtcgtttg ggttacgggc gctgatggat
     2101 ttattggatc ggctttggtt cgaaatctgg ctcattcggg gattcgtacc ttgatgccgt
     2161 cattagatat tagggatacg gcggcgattc aaaaagtcgt ttccgagaat tcctttgatg
     2221 cggtcattca ccttgcgggc atgagttcgg ttaaggagtg cgaaataaat aagtcgctcg
     2281 cgttttctgt caactcggaa gctgtgtacc acttgggcga ggctatagct aaaattaaac
     2341 cacaggctca acttattttt ccgagcacag ggcatgttta tcaatcctat ccgatgggaa
     2401 cacaagaaga gatctatacc gagaagtcgt ccgtcttgcc caggaacttc tatgctttga
     2461 ccaaactgca ggctgaagaa aatcttaagt ttttagcaaa aaataggggt ttaaaggcaa
     2521 ctgttttgag gctgtttaat cacacccaca aatctcaaac tcaagatttt tttcttccct
     2581 ctgtatacgg tcagttgtta agtgcgaaaa aacggggtga aaaaaaagca ctgctgacag
     2641 tgggtgatat tgacgtccga agagatatag gtgcaattca agatttagcc ttggcatttc
     2701 agacggtttt atttgagaat ccatataaaa gcgatgaggt gaagactttc aacatttcat
     2761 caggtcatgc tgtgggtctc agagatttgg tgaatcaatt ggctgacaag atggaaatgg
     2821 aagtggattt taaggtggat cctcaattga ttcgggagaa tgaaccaaaa tcagtggttg
     2881 gaataaattc t
//
LOCUS       DATBTE010000166         5955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_294082, whole genome shotgun sequence.
ACCESSION   DATBTE010000166 DATBTE010000000
VERSION     DATBTE010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294082"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1622)
                     /locus_tag="VIG33_06230"
     CDS             complement(111..1622)
                     /locus_tag="VIG33_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEY8270468.1"
                     /translation="MSWFNTITEWLTLHHYGFSLNAYSLAFGGLILFTLAVVAKFSRN
                     YLKGEVGEVRFWLLFLFFAMGILTSCFAENLKTFYVGWEMVGLASFFLIGFYQTNSRS
                     IENSLIALSNYKICDLFFVMAILLEEANFYTGSGLCLIIATLAKSAQLPFSSWLYRAL
                     EGPTPSSTLFYGGLSLHLGAFVLLQFSPLWDHSGWLRLLVGSIGLASAIFGFLVGSVR
                     SDLKTSFAFASISQVGLIYCEMAMGFYNLAMWHIIGHNLLRTWNYLRGMSFFDEFFRE
                     EYHQQNNWLLQKVFTSLPESFFFHSMNSFYLDRFSVFLRKWGLLVFAIGGTIGLAMSP
                     EGDHYAFLGLGLALLVTVALFFKESLKPVNFLIGLVASQILFLASIHGFLEDKLEHLY
                     LFVCLFSLGLLLFSLWPAVTLWIKQQDAGNEKQNFLGLATKLHWRHMVFLVAAILITG
                     SPGTFEFFVQEGIIDEIWSRSHLFLVVAMVCLTLNTVHTFKIGQLAFLGEPNS"
     gene            complement(1609..3858)
                     /locus_tag="VIG33_06235"
     CDS             complement(1609..3858)
                     /locus_tag="VIG33_06235"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative inorganic carbon transporter subunit
                     DabA"
                     /protein_id="HEY8270469.1"
                     /translation="MEHQQELKEILRELRPQLPIQNPIPFFVHNNPLQYWEKFKFEQG
                     IAASCLLYERAGIQVDRTFVREFEDIVIPVLSAYLDQGMRRWSGQSHFLEQGLWSWFI
                     SFVKYSSRQKSKSFYKIKKGLDSLALETLSFDGAEEFICNSLKIRFGPRNYKKKYFYI
                     KSLLFHFKGWSGMVHLLESNPQLFPLQKKKVSLIDWLAILIMVETKLSDSAEDSVVVD
                     LELRHRRQDELNVQIQGIKRREFAFYGSIIRQFKSQLDHLKQLKKTEPLQSPRSFAQV
                     FFCIDDREEALRRILEKVNPQIETFGTVGFFGVDVSIKSMGQLVFQPSCPPVIQPRKK
                     VVETALRETEPLAKTTRNLATAFNHTKLNLLEPLIALFAPIFYLVSLLLRSSHIGFYQ
                     RLKNKVLKNPNKSHGLNYEFVPGFFYSAEEKAKIVMDILKSAGLKNNFAPVVVMMAHG
                     ASTTNNPFQKSYGCGACSGQSGFPNAKIFCSFANDDQIRELLKEKGYLIPKDTLFLAA
                     HHDTCSDEIMISDKKGMAEVQASIVEQLRLDFKEALKQNTQVRFGRFNIKPTESPLHR
                     SLDWSQPRPEYGHSKVALVVFGPRSLTCGLDLDGRAFLVSYDPATDVDGAELEFVIMN
                     ALPVCANINLDYFTSGAFPEAFGSGSKLPMNIVSGIGLMTGSKSDLKIGLARQMVDQH
                     EALRLLAFVCCKKEHLLNTISKSSRLTNLINNEWIHLMRIDPVDFSLEPITQEISKEF
                     SQEFADELV"
     gene            complement(3870..5414)
                     /locus_tag="VIG33_06240"
     CDS             complement(3870..5414)
                     /locus_tag="VIG33_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY8270470.1"
                     /translation="MSMKSMKIMRNLKTDLPASLVVFLIALPLSLGISLASGAPMKAG
                     LISASIGGIVVGLLTGAPLQVSGPAAGLSVMVFGFIQKFGFDVTCVITIIAGLTQLLF
                     GFLGAAQLAFVISPAVIHAMLAGIGILIALGQVHVLLGFGPKSSALLNMAGYPDSLTH
                     MNTSAAMLGLFSLAVLFCWNKWVQKKIPVIPGSLVAVVTGTLLGIFSHADVPHVQIGT
                     QLFDGMSSLKMGDVPVWELIVSGLGLALVASAESLLCAVATDKLHSGPRVNLGKELVA
                     QGVGNTLAGFIGGLPITGVIVRSSANVSAGAKSQISSVLHGVWILVFAVFFTPVLNQI
                     PLAVLAALLIYTGVKLVKIAEVKKLMAYQETLVYFATLLGVVFINLLWGIGIGFGLAA
                     VLMVARMAKFQFQNELDKDKKVARVDISGNLTFLAVPKLLKQLNALPKKYEVQMSLQV
                     DHLDHSVVEAMHDWKQNYEKDGGKVIKASLVDLWKDLKTPEVNRRKPKPKYFKPQEHS
                     ISEISL"
     gene            5624..>5955
                     /locus_tag="VIG33_06245"
     CDS             5624..>5955
                     /locus_tag="VIG33_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8270471.1"
                     /translation="MKLLIIEDQHSVAQYLKKGLSESGYIVDLAHEGEEGFLIATSRS
                     YDLIIVDGLLPKIDGWSLVKKLRSAGMAIPILFLSARDEVDDRVRGLESGADCYLVKP
                     FAFSELLA"
BASE COUNT         1909 a         1398 c         1179 g         1469 t
ORIGIN      
        1 cgagtttcaa aatgaataat aagcgccttc gcgacttatg atagccgtat cttcgtctag
       61 tagaaaattt aagacccttc tataaaacgg agagcccccg gaacgaaagt ctaagaattc
      121 ggctccccca gaaaagccag ctgaccgatt ttgaaggtat ggaccgtatt cagtgtcagg
      181 caaaccatgg ctacgaccaa aaataaatgg gaccttgacc agatttcatc gatgatcccc
      241 tcttgaacaa agaactcgaa ggttcccgga gagcccgtaa tcaaaattgc cgccactaag
      301 aacaccatgt gccgccaatg tagctttgtg gcgagtccta agaagttttg tttttcatta
      361 ccggcatctt gctgttttat ccataaagtc accgcaggcc acaaagaaaa gagcagtagg
      421 cctaaactaa aaagacagac aaataaatac aagtgctcca acttatcttc aagaaaaccg
      481 tggatagagg ctaaaaataa aatctgagaa gccactaagc ctattaaaaa atttacgggc
      541 ttcaggctct ccttgaaaaa caaggcaacg gtcacaagca aggccaggcc taagcctaaa
      601 aaagcataat gatcgccttc gggggacatt gccagaccaa tagtccctcc gattgcaaat
      661 acgaggagtc cccattttcg caaaaaaact gaaaagcgat ccagataaaa actattcatg
      721 ctgtgaaaga agaaagactc aggcagagaa gtaaaaactt tctgcaacaa ccaattattt
      781 tgttggtgat attcctctct gaaaaattca tcaaagaaac tcattcctcg aagataattc
      841 caagtcctca aaagattatg gccaataata tgccacatcg ctaagttata aaatcccatg
      901 gccatttcac aatagataag gcctacctga gaaatcgagg caaaggcaaa agaagttttc
      961 aaatcactgc gcacggatcc gaccaagaac ccaaaaatag cgctcgccag acctatactc
     1021 cccaccaaga gcctcaacca tcccgagtga tcccagagag gagaaaactg taagagaacg
     1081 aaagcgccca aatgaagtga aagtcctccg taaaaaagcg tactggaagg cgtaggtccc
     1141 tcaagggcac ggtaaagcca cgacgaaaaa ggcaattgag cactcttggc caaggtggca
     1201 atgattaaac acaagccact gcctgtgtaa aaattcgctt cttccagaag aatagccatg
     1261 acaaaaaata aatcacaaat cttgtaattg gataaagcaa tcaaagaatt ttcaatgctt
     1321 cgagaattcg tctgataaaa accgatgagg aaaaaggagg ctagaccgac catttcccag
     1381 ccaacataaa aagtcttcaa attctccgca aaacaagagg tgagaatacc catcgcaaaa
     1441 aataaaaaaa gcagccaaaa gcggacttca ccaacttcac ctttgaggta atttctggaa
     1501 aacttggcta ctacggccaa agtgaaaaga atcaatccgc caaacgccag cgagtaagca
     1561 ttgagagaaa atccatagtg atgtaatgta agccactctg taatagtatt aaaccaactc
     1621 atctgcaaac tcctgggaaa actctttgga aatttcctga gttattggct ccaatgaaaa
     1681 atcaacgggg tctattctca tcaaatgaat ccattcattg ttaattaaat tggttaagcg
     1741 cgaagatttc gagatcgtat taagcaaatg ctctttctta cagcacacaa aggccagtaa
     1801 gcggagagcc tcatgctgat ctaccatttg ccgagcgagc ccgattttaa ggtcgctttt
     1861 tgaacctgtc attaaaccaa tcccagaaac aatattcata ggaagcttgc tgccagaacc
     1921 gaaagcttct ggaaaagctc cagaggtaaa ataatccaga tttatattcg cacacaccgg
     1981 aagtgcgttc ataattacaa attccaactc agcgccgtcc acatcagtgg ccgggtcata
     2041 ggagactaag aaagcacgtc catccagatc caatccacag gtcaatgagc gtggtccaaa
     2101 cacgaccagc gccaccttag aatgcccgta ttcgggacgc ggctgggacc aatctaaaga
     2161 tcggtgcaga ggagattccg ttggtttaat attaaaccgg ccgaatcgga cttgcgtgtt
     2221 ctgcttcaag gcttctttaa aatctaaacg caactgctcc acgattgaag cctgcacctc
     2281 agccatgcct ttcttatccg agatcataat ctcatcggag caagtgtcat gatgggccgc
     2341 taagaacaaa gtatccttcg gaatcagata acctttttct ttaagaagct ctcggatctg
     2401 gtcgtcattg gcaaaagagc aaaaaatctt cgcatttgga aaaccggatt gcccgctgca
     2461 agcgccacaa ccataggatt tttgaaaagg attatttgtt gtggaagccc catgagccat
     2521 catcaccaca accggcgcaa agttattctt taagcccgca gacttaagga tatccatgac
     2581 gattttagct ttctcttcag cggaataaaa aaatccaggt acgaattcat aatttagacc
     2641 atgagacttg ttcggattct taagaacctt atttttaagt cgctgataaa accctatgtg
     2701 agaagacctg agcagcaaag acactaaata aaatatcggt gcgaacaggg caattaaggg
     2761 ttctaataaa tttaacttcg tatgattaaa agcggtcgcg agatttcttg tcgtcttagc
     2821 cagtggttct gtttctcgaa gagcggtctc aaccaccttc ttacgcggtt gaatgaccgg
     2881 aggacaggac ggctgaaaaa ccagttggcc catggacttt atggacacat caacgccgaa
     2941 aaaccccacc gtgccaaagg tttcgatttg aggatttact ttttctaaaa ttctgcgtaa
     3001 ggcctcttcg cgatcatcaa tgcagaaaaa aacttgggca aaacttcgag ggctttgcag
     3061 tggctccgtt ttttttaatt gctttaaatg gtcgagctga gatttaaact gacggataat
     3121 tgagccataa aaagcaaatt ctcttctctt aatcccctga atttgaacat tcaattcatc
     3181 ttgacgacga tggcggagtt cgagatccac tacgacggaa tcttccgctg aatcactcaa
     3241 cttggtctct accatgatca gaatggccaa ccaatcaatc aacgagactt tcttcttttg
     3301 cagcggaaac aactgtgggt tcgattcaag aagatgaacc atcccactcc agcccttgaa
     3361 atgaaaaagc aatgatttga tataaaagta tttctttttg taattcctgg gaccgaatcg
     3421 aattttcaaa gaattacaaa tgaactcctc agcgccatca aaactcaaag tctccaacgc
     3481 caaggaatcc aagccttttt taatcttgta aaacgattta cttttttgtc gcgagctgta
     3541 tttgacaaag ctgataaacc aactccacag accttgttct aaaaaatgac tttgtcctga
     3601 ccaacgacgc atcccctgat caagatatgc cgaaagcaca ggaataacga tgtcctcaaa
     3661 ctctctgaca aaagtgcgat ccacctggat ccctgctcgc tcataaagta aacaggaagc
     3721 cgcaatcccc tgctcaaatt taaacttttc ccagtactga aggggattgt tgtgaacgaa
     3781 gaaagggatg ggattttgaa tggggagttg gggtctcaac tcccgtaaaa tttctttaag
     3841 ttcctgctga tgttccataa attttaatac tacaatgaaa tttcggaaat ggaatgttcc
     3901 tggggtttaa aatactttgg tttcggcttt cgcctgttca cctcaggagt cttcagatct
     3961 ttccaaagat cgaccaatga ggctttgatg actttaccgc catctttttc atagttttgt
     4021 ttccaatcgt gcatggcttc gaccactgaa tgatccaaat gatccacctg caaagacatc
     4081 tgtacttcat attttttagg cagcgcattg agctgcttca aaagcttggg caccgccaag
     4141 aaagtaagat ttccgctaat gtccacgcgc gctacttttt tgtccttatc taattcattc
     4201 tgaaattgaa acttcgccat tcgcgcaacc ataaggactg ccgccaaacc aaatccaatc
     4261 ccaatacccc aaagaagatt gatgaagaca actcccaaga gcgtcgcgaa ataaacaagg
     4321 gtctcttgat aagccatgag ttttttgact tcagcaatct taacgagttt aactcctgta
     4381 tagattaata gagcggccaa gacagccaaa ggaatctgat tcaatacagg ggtgaaaaaa
     4441 acagcaaaca ccagaatcca aactccatgc agaactgatg aaatctggct ctttgctccc
     4501 gcggatacat tcgcagaact tcttacaatc acaccagtga tggggagacc cccgataaat
     4561 cccgccaatg tattaccgac cccttgagca actaattctt ttcctaaatt cacccgggga
     4621 ccactatgaa gtttgtcagt ggccacggca cacaaaagag actctgcact cgccactaaa
     4681 gcaaggccca ggccgctcac tatcaattcc cacacaggga catcgcccat ttttaaagaa
     4741 ctcattccat caaaaagctg agtgccaatt tgtacatgcg gcacatccgc atgggaaaaa
     4801 atacctaaca gagttccagt aactaccgcg acaagggagc caggtatcac tggaatcttc
     4861 ttttgcaccc atttgttcca gcaaaataga accgccagac taaaaaggcc caacattgct
     4921 gctgaggtat tcatgtgagt cagagaatca ggataaccag ccatattcag caaagctgag
     4981 ctctttggcc caaaacctaa aagcacatga acttggccaa gagcaatcaa aataccaatc
     5041 cccgcaagca tcgcatgaat caccgctgga gaaattacaa aagccaactg cgcagcgccg
     5101 agaaaaccga acagcaattg agttaagccg gcgataatgg tgatgacaca cgtgacatca
     5161 aagccaaact tttgaataaa accgaagacc atgacagaaa ggcctgcagc cgggccactt
     5221 acctgcagtg gagctcccgt taggagacct acaacaatcc ctccgataga ggccgaaata
     5281 agtccggctt tcatcggagc tccggatgca agagaaatgc ctaaggagag gggtaatgca
     5341 atcagaaaca ccactaaaga ggctggtaaa tctgtcttta aattcctcat tattttcatg
     5401 cttttcatac tcatatggat aacccccaaa attttgcctt gtcgttattt cactagcctt
     5461 aaatattgat ttcgcaaatt atttcagctg tcagtactgg cgtagttgca attttattgc
     5521 caaagctccc tattgacttt ttattgaata aagaagatat ccgacgaaaa ttaaaatcaa
     5581 attactggaa tattacattt ttgtcattta ctgcggagtc tgaatgaagc tacttatcat
     5641 cgaagatcaa cattccgtag cgcaatatct gaaaaaaggg ctctccgaga gtggctacat
     5701 cgttgatcta gcgcatgaag gtgaggaggg ttttttaata gccacctcta gatcctatga
     5761 cctcatcatt gttgatggat tgctccccaa aatagatgga tggagtttag ttaagaaatt
     5821 acgaagtgca ggtatggcga ttcccatcct cttcctttcc gcacgggatg aggtcgatga
     5881 ccgagtccga ggtctagaat ccggagctga ttgttacctc gttaaacctt ttgccttctc
     5941 cgagctctta gctca
//
LOCUS       DATBTE010000167         8584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_157508, whole genome shotgun sequence.
ACCESSION   DATBTE010000167 DATBTE010000000
VERSION     DATBTE010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 8584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8584
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157508"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VIG33_06250"
     CDS             complement(<1..512)
                     /locus_tag="VIG33_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HEY8270472.1"
                     /translation="MKSKSDLKSDVMSDLIAKYDVPVPRYTSYPTVPYWTEAPTPEQW
                     IQHLRQTLKVSENRWSMYMHLPFCESLCTFCGCNNSITKDHKKEMPYIQMVLKEWNLY
                     KAQVPELLERPLNHLHLGGGTPTFFSPESLKLLISTLWKDIKKDSEHFEASIEVDPRR
                     TTVEHLKVLK"
     gene            complement(509..1531)
                     /locus_tag="VIG33_06255"
     CDS             complement(509..1531)
                     /locus_tag="VIG33_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEY8270473.1"
                     /translation="MSNINFQNALQRKIQNVPPIWFMRQAGRYHKHYQNLRRDHSFDD
                     LCKKPELAAQVALGPVEDFDFDVAILFSDILYPLQALGMGLEYTDHGPKLGFSLTPEN
                     IGNLKRGEDVVSFMNFQKEALQLTRTVLPANKSLIGFMGGPWTLFVYAVEGSHAGSLT
                     QSKKLISLFPEFLKKMLPLLKENIRLQLAGGAEVVMIFDTAAGELSPLDFKKWVQPVL
                     KELNALAAGKIGYYSKATQTAYFNQEFLDLPWAGMGFDHHWNLPECFKLAKQGFVQGN
                     FDQSLLFLDKASFSEELGIYLENMKSLSAAERVGWVSGLGHGVLPKTPEENVRSFVDT
                     VRKVFG"
     gene            complement(1545..2219)
                     /locus_tag="VIG33_06260"
     CDS             complement(1545..2219)
                     /locus_tag="VIG33_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8270474.1"
                     /translation="MRKILLVEDDTSLGETLKERLSKDYQVLWAQSVRAAKETLRLEG
                     NLDLVILDIGLPDGLGFEVAEYIKAQNMHPLFIFLTAQADAEARLKGFELGAEEFIPK
                     PFHLKELLLRVKHVLDLHAPQEEIVIEGVVINFTSLSVKRPNGQIEYPPMTDIKILQL
                     LIDRAPHVVSRDEIINEVWGLDKNPSHRTVDNTIVRLRQLIGENGEAYIRSVRGVGYQ
                     WFNNQI"
     gene            complement(2216..3058)
                     /locus_tag="VIG33_06265"
     CDS             complement(2216..3058)
                     /locus_tag="VIG33_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8270475.1"
                     /translation="MNKIKTLLATIWVLFVVSLVSWWWVYLTSSLEAGVEMNKRHRMF
                     MWEGSMLLVALIIGGAALVFLSYRDEKRHEQLKTFFATFSHDIKTSITRLRLQAEILE
                     EDNESQKNPVLQRLITDISRLDLQLENSLLLSTTGAQNLLLEKIALSKLIESLRIQWS
                     ELEFRLNQEAEIVADQRAIVSVFRNIIQNAVLHGQATVIEFSAKALSSQRVEISIQDN
                     GKGFSGDKECLGVRMLPSKNGKGSGVGLYLCHRLLKKMNGQLSFENSSQGLRAVIQIA
                     GRLL"
     gene            complement(3055..4338)
                     /locus_tag="VIG33_06270"
     CDS             complement(3055..4338)
                     /locus_tag="VIG33_06270"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY8270476.1"
                     /translation="MQSLELFERAKKVVPGGVHSPVRSFKGLDHAPVFFKNAQGAFLT
                     SVEDKTYIDFCQSFGPLILGHRDAEVAEEVHRMIDTAWTFGACEIYSLELAEWMTQNL
                     PWVEKLRFVSSGTEAVMSALRVARAATHRTKILKFEGCYHGHADQLLVKAGSGLAGSS
                     ASSSAGISAEVAATTLVTPLNDIEKLREVFTTQGKEIAAVIIEPLPANYGLLVQKPEF
                     LNEVKNLCQANGALLIFDEVISGFRTALGGMVELTGLKPDLVTYGKVIGGGFPVGCYG
                     GRKDLMDLVAPSGEVYQAGTLSANPVGMRAGLRTLQKMKALDGWKVLNQRTLRFCQKL
                     QKGFDEMKAPVTVSYHSSLFWIHGRVNEPIRRIEQIPPGQGENFKKLFLKCLEKGVYL
                     APNAYEVGFLSLAHTEAVLDEAADKILQAAREVFI"
     gene            complement(4326..5828)
                     /gene="hemC"
                     /locus_tag="VIG33_06275"
     CDS             complement(4326..5828)
                     /gene="hemC"
                     /locus_tag="VIG33_06275"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEY8270477.1"
                     /translation="MRLGACLRISARKSDLARLQAYQVGEVLQKKIPGIEIQYSFRES
                     LGDKNLTDPLWKMPEKGVFTEDFVQDLLEEKTDLVVHSWKDLPTAPRIGTQIVATLPR
                     ADQRDLFLFKKNHDERVRSSKALHVFSSSPRRAYNLENFLKVHLPYDLKEVQFHNIRG
                     NIQTRVRKMLEDPAVDGLIVAKAAIDRLLTVKASEFNETRSFLRETLQQLNWVVLPLS
                     VNPNAAAQGALAIEIKNGRSDLEKILSQVNCAETFASAQKERETLAGFGGGCHQKIGV
                     AVLKRSYGDVFFLKGLTDQGQILDEKSLLREFKSAKLESTQLASSKELKAVRSHSLSV
                     NIPDAIQGLWVAKAEAFPEPLQFSGLVWTAGLQTWKKLAQRGIWVHGSSEGLGEDENP
                     QIETLFGSVIRWAKLTHKDAPQEPGKEVIATYEVIVSAEAWQMGNREAFYWNSGSQFL
                     AALKKEPGLIHKRHACGPGNTYKVLRDQLGPQASIEIYLDEENWRDQCSR"
     gene            complement(5819..6697)
                     /locus_tag="VIG33_06280"
     CDS             complement(5819..6697)
                     /locus_tag="VIG33_06280"
                     /inference="COORDINATES: protein motif:HMM:NF017051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270478.1"
                     /translation="MQDILLLHRHSHKVFSGIVDQSLVWKTCLRQIAFLPEGSDLPWE
                     EGDEIYSGAEAFAFLLEIICGLKSPIVGETEIFGQFKNFTSKALESKNAMTLQLRGFF
                     QSLIQMAKSLRTEHLVGLGSQGYGSLVRKLSKNDPAVTLLGSGQLVEEILPWLSKEKA
                     LQVVCRHPEKAQRFKEKYSTLKIDSLDGTPELYACLVVAAPLTDEELLRWLNSHQASH
                     PVGKILDLRGELERPELFHEYQFIGLKDVFRNIEENKKDLSQRVITLKDLVRQRAQDY
                     FERCLHRPFGWEDLCV"
     gene            6836..7813
                     /gene="hemB"
                     /locus_tag="VIG33_06285"
     CDS             6836..7813
                     /gene="hemB"
                     /locus_tag="VIG33_06285"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY8270479.1"
                     /translation="MDITYRPRRNRRTPALRNLIAETSLSVDQFIYPLFLCEGDQQKV
                     AIKSMPGQFRLSADYLLKEISECQSLGIHSFALFPAINETLKNSQGKESFNPAGLLPT
                     TVKKIKDKFPDSCIITDVALDPYSSDGHDGIVKDGQILNDETIEILVKMSLTQAEAGA
                     DMVAPSDMMDGRVGAIRRALDSEGFTQTGILAYSAKYASSFYGPFRDALESAPKFGDK
                     KTYQMDPRNSREALLEAELDIQEGADMIMVKPALAYLDIIQLLKQNTTLPIAAYNVSG
                     EYAMIKAAAQNGWLDGEKAMMESLISIRRAGADIILTYFAKEAALVLNR"
     gene            complement(7810..>8584)
                     /locus_tag="VIG33_06290"
     CDS             complement(7810..>8584)
                     /locus_tag="VIG33_06290"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HEY8270480.1"
                     /translation="KELGDSFDIMAALSWGTLPKIFSLAEVEDKIDYLNSYCDAYLRE
                     EIIAEQIVRNVTPFRHFLEVAAQGNSKIINYSNIAKSIGVDTTTVQNYFQILEDTFAG
                     VLLQPFHESIRERQRKNPKFYFFDTGVTRSLQNRLTLPLVEQSFEFGDLFEQFIILEF
                     IRLNFYFKKSWKFSYLMTKNNVEIDLIVERPGAQRLLVEIKSTDAIQNLPDSHWVGFR
                     KIVKDFPNALGVCVSRDPVAFVKDGIRFVHWKQLFDELF"
BASE COUNT         2415 a         2186 c         1718 g         2265 t
ORIGIN      
        1 tctttcaaaa ctttgagatg ctccaccgtc gtgcgccgag ggtccacttc aatggaagct
       61 tcaaaatgct cggagtcttt tttgatatcc ttccagaggg ttgaaattaa caacttcaaa
      121 ctctcaggtg aaaagaacgt cggagtcccc ccgcccaagt gcaaatgatt cagagggcgc
      181 tccagaagct ccgggacctg ggctttataa agattccact ctttcagaac catctgaata
      241 taaggcattt ctttcttgtg atccttggta atgctgttat tgcagccgca gaaagtgcaa
      301 aggctttcac agaaaggcaa gtgcatatac atcgaccagc gattctccga caccttcagt
      361 gtttgacgaa gatgttgaat ccattgctcc ggagttggag cctccgtcca ataaggcacc
      421 gtgggataac tcgtataacg aggcaccggc acatcatatt tagcaattaa gtcagacatc
      481 acatcagatt tcaaatcaga ctttgatttc atccgaacac ctttcgtact gtgtccacaa
      541 aggaccttac attttcttcc ggagtcttag gcaaaactcc atgaccgagt ccactgaccc
      601 agccgactct ttccgccgct gacaaggatt tcatattctc tagataaatt ccgagttctt
      661 ccgaaaagga ggctttatct aaaaacaaca aactttgatc gaaattcccc tgcacaaaac
      721 cttgctttgc caacttaaaa cattctggca aattccaatg gtgatcaaaa cccattcccg
      781 cccagggcaa atccaaaaac tcttggttga aataagccgt ttgagtggct ttcgagtagt
      841 agccgatttt ccctgcagct aaagcattga gttcttttaa cacaggctgc acccattttt
      901 taaaatccaa cggagacaac tcccctgccg ccgtatcaaa aatcatcacc acttccgccc
      961 cacccgcaag ttgcaggcga atattttctt tcagcagtgg taacatcttt ttcagaaact
     1021 ccggaaaaag ggaaataagt tttttagact gagtcaagct cccggcatgg gatccttcca
     1081 ctgcataaac gaataaagtc cagggaccgc ccataaaacc aataagactc ttattggcag
     1141 gcaaaaccgt gcgcgtcagt tgcagagctt ctttttgaaa attcataaag gacacgacgt
     1201 cttcgccacg ctttaagttt ccgatatttt ccggggtcaa agaaaagccc agtttcggac
     1261 cgtgatcggt gtactccagt cccatgccca gggcttgaag gggatacaga atatcactaa
     1321 ataaaatcgc cacatcaaaa tcaaaatcct ctacaggccc caaggccact tgcgccgcaa
     1381 gttcgggctt tttacaaaga tcatcaaagc tgtgatccct gcgaagattc tggtaatgct
     1441 tatgatagcg tcccgcctga cgcataaacc agataggagg aacattctga attttacgtt
     1501 ggagtgcatt ttgaaaatta atattcgaca tagaacattc cttactaaat ctgattatta
     1561 aaccattgat aacccacacc ccgcaccgag cgaatgtaag cttcaccatt ttccccaatc
     1621 aattggcgaa gacgaacgat ggtattgtcc accgtgcgat ggcttggatt tttatcaagt
     1681 ccccaaactt cattaataat ttcatctcgg ctcaccacat gtggcgcccg atctatgaga
     1741 agttgcaaaa tcttaatatc ggtcataggg ggatactcaa tctggccatt gggccttttg
     1801 accgagagac tggtaaagtt gatcaccact ccttcaatca caatctcttc ctggggagca
     1861 tgcaagtcca agacgtgctt gacccgaagg agaagttctt tcaaatgaaa tggctttgga
     1921 ataaattctt ccgcgcccaa ttcaaatcct ttaaggcgag cttccgcatc cgcctgcgcg
     1981 gttaagaaaa taaacaaagg atgcatattc tgagccttga tatattcagc aacttcaaaa
     2041 cccagcccat cgggcaagcc aatatccagg atgactaaat ccaggttccc ttccaaacga
     2101 agagtttctt tggccgcgcg aacactttgc gcccaaagga cctgataatc ttttgaaaga
     2161 cgctctttca gcgtttctcc tagggaagtg tcatcttcga ctaatagaat ctttctcaaa
     2221 gaagtctccc tgcaatttga ataactgcac gcaatccttg agagctattt tcaaagctca
     2281 gctgaccatt cattttttta agcaagcggt ggcataaata caaaccaacg ccgctgcctt
     2341 ttccgttttt ggaaggcaac attcgcaccc ctaggcattc tttatcaccc gagaagcctt
     2401 tgccattatc ttgaatggaa atttccactc tctgagaact taaagccttg gccgaaaatt
     2461 ctatcaccgt ggcctgtccg tgcaaaaccg cattctgaat aatattgcga aagacgctga
     2521 caatggcccg ttgatccgcc acaatctccg cctcttgatt caatcgaaac tcgagttcac
     2581 tccattgaat gcgcaacgac tcgatcagct ttgacagtgc tatcttttcg aggagaagat
     2641 tctgagctcc tgtcgtggaa agcaaaagag agttctcgag ttgaagatcc aaacgcgaaa
     2701 tatcagtgat aagccgctgc aaaacagggt tcttctggga ctcattgtct tcctctagaa
     2761 tctcggcttg aagcctgagg cgggtaatag aggttttaat atcatgactg aaagtggcaa
     2821 aaaaagtctt gagctgttcg tggcgttttt catcacggta ggatagaaaa accaaggccg
     2881 ctccaccgat aatcagcgcc accaacagca tgcttccttc ccacatgaac atacgatgtc
     2941 gcttgttcat ctcgacgccg gcttccaagc tgctggttaa ataaacccac caccagctga
     3001 ctaaagagac aacaaataaa acccaaatcg tggctaaaag ggtttttatt ttattcaaat
     3061 gaaaacctct cgagcggctt gcagaatttt atccgcagct tcgtctaaaa ccgcttctgt
     3121 atgcgccagg gacaaaaacc ccacttcgta ggcattgggg gccagataaa cacctttttc
     3181 caaacatttt aaaaacaatt tcttaaagtt ttcgccctgg cctggcggga tctgttcaat
     3241 cctgcgaata ggttcattca cgcgtccgtg aatccaaaac aaagaactat ggtaagaaac
     3301 cgtgaccggc gccttcattt catcaaaacc cttttgcaac ttttgacaaa accgcaaagt
     3361 tctttgattc aaaactttcc agccgtccag agctttcatt ttttgcaaag ttcgcagacc
     3421 cgcacgcatt cccacaggat tcgcactcaa ggttcccgct tgatagacct caccgctcgg
     3481 tgccaccaga tccataagat ctttgcgccc cccgtagcac cccaccggaa aacctccgcc
     3541 aatgactttg ccataagtca caagatccgg cttaaggccc gtcagctcaa ccatgcctcc
     3601 cagtgccgtt ctgaatccag agatcacctc atcaaagatc aataaggcgc cgttggcttg
     3661 gcaaagattt ttcacttcat tgaggaactc tggcttttgc accaaaagtc cgtaatttgc
     3721 cggaagcggt tcaataataa ccgccgcaat ttcttttcct tgtgtggtaa agacttctcg
     3781 cagcttttca atatcgttca gaggcgtgac cagagtcgta gccgctacct ccgcagagat
     3841 cccggcgctg ctggaggcgg agcttcccgc caacccactg cccgccttca ccaataattg
     3901 atcggcatga ccatgataac agccttcaaa ttttaaaatt ttcgtacggt gagtggccgc
     3961 acgcgcgacc cgcaaagcac tcatcaccgc ctctgtgcct gaggaaacaa aacggagttt
     4021 ttccacccaa ggaagatttt gcgtcatcca ttcggccagt tccaaagaat agatttcgca
     4081 agccccgaaa gtccatgcgg tgtcgatcat gcgatgaact tcttccgcca cctccgcatc
     4141 tcggtgcccc aaaatcaagg gaccaaaact ttggcaaaaa tcaatataag ttttatcttc
     4201 cacacttgtc agaaaggcac cctgggcatt tttgaaaaaa acaggagcat gatccagacc
     4261 cttgaaagaa cgaaccggag aatgaactcc accgggtact acttttttcg cacgttcaaa
     4321 aagttctaac gactgcattg gtctctccaa ttctcttcat ccaaataaat ttcaatcgat
     4381 gcctgaggcc ctaattgatc ccttaatact ttatacgtgt ttccaggacc acaggcatgt
     4441 cttttatgaa taagacctgg ctcttttttt aaggccgcta aaaactgtga accactgttc
     4501 caataaaatg cttcgcgatt tcccatctgc caagcttcag ctgaaacaat cacttcatag
     4561 gttgcgatca cttctttacc gggctcctgt ggtgcatctt tgtgagttaa tttagcccag
     4621 cgaataacag agccaaaaag agtttcgatt tgaggatttt catcttcacc caatccttca
     4681 ctggagccat ggacccaaat acctcgctgc gctaattttt tccatgtttg taatcccgca
     4741 gtccacacca gtcctgaaaa ctgcaacggc tcggggaagg cttccgcctt ggccacccaa
     4801 aggccttgga tagcgtcagg aatatttact gatagtgaat gactccgcac ggctttcagt
     4861 tctttggagc tggccagttg agtggattca agcttcgcac tcttgaactc gcgcaggagc
     4921 gatttttcat caagaatttg cccttgatcg gtcagaccct ttaaaaaaaa cacgtctcca
     4981 taagagcgct tcaaaactgc cacaccgatt ttctggtggc aaccgccgcc gaaaccggcc
     5041 aaagtctccc gttctttttg cgcactggcg aaagtctcgg cgcagttcac ttgcgaaaga
     5101 attttttcca aatcacttcg accgttttta atttcgatgg ccaaggcccc ttgcgccgct
     5161 gcattgggat tcacagaaag gggcaatacc acccagttca gttgctgaag agtctctcgc
     5221 aggaagctcc gagtttcatt aaactccgaa gccttcactg ttaacaaacg atcgatagcg
     5281 gccttggcta cgatcaaacc atcaacggcc ggatcctcga gcatttttct gacgcgggtt
     5341 tggatattgc cacgaatatt atgaaactga acttctttta aatcataggg aagatgaact
     5401 ttaagaaaat tttctaaatt ataagctcgg cgcggagaag agctgaaaac gtggagtgct
     5461 ttactggaac gaacccgttc atcatgattt tttttaaata agaacagatc acgctggtcg
     5521 gccctgggca aggtcgccac aatttgggtg cctatacgag gcgccgttgg caaatccttc
     5581 caggaatgca ccaccagatc tgttttctct tccaataaat cttgcacgaa gtcctctgtg
     5641 aaaacccctt tctcgggcat cttccacaaa ggatccgtta aatttttatc acccagagat
     5701 tcgcgaaaac tgtattgaat ttctattcca gggatttttt tttgaagaac ttcccccact
     5761 tggtacgcct gcaagcgcgc caagtcgctt ttacgcgcag aaattcttaa acacgctcct
     5821 aaacgcacag atcctcccag ccaaagggac gatgcaagca gcgctcaaaa taatcttgag
     5881 cccgttgccg caccaaatcc ttgagagtga tcactctctg actgagatct ttcttatttt
     5941 cttcaatgtt tctaaaaaca tctttcaaac cgatgaattg atattcgtga aaaagttccg
     6001 gccgctccaa ttcaccacga agatcaagaa ttttcccaac cggatgactt gcttgatggg
     6061 agttcaacca gcgcagcagc tcttcatccg tcagcggagc ggccacgacc aggcaagcat
     6121 aaagttctgg cgtcccgtcc agggaatcaa tttttaaagt cgaatatttc tctttaaagc
     6181 gctgggcttt ttcgggatga cggcaaacca cttgcagagc tttttctttt gaaagccagg
     6241 gcagaatttc ttccaccaat tggcccgatc ccaagagcgt cacggcggga tcattcttgc
     6301 tcagtttacg caccagactg ccataacctt gtgagcccaa gcctactaaa tgttccgtgc
     6361 gaagactctt cgccatttga atcaaagatt ggaaaaatcc acgcaattgc aaagtcatcg
     6421 catttttcga ctccaaagct ttgctggtaa aatttttaaa ttggccaaag atttcggttt
     6481 cccccacgat gggagatttc agaccacata taatctctaa taaaaatgca aaagcttcgg
     6541 caccggaata gatctcgtcc ccctcttccc agggaagatc gctcccctct ggtaagaaag
     6601 caatttgccg caaacaggtc ttccaaacca aactttgatc aacgattcct gaaaatacct
     6661 tatgactatg gcgatgaaga agtaagatgt cttgcatgat aaatttgtaa cgtatttcca
     6721 cctgccaatt gtcatttaat tgtcatttag aggctatttt ttataaaaat ggctcaatgc
     6781 gaaacatgtc tcggaagaag cctttacttc tagctaggat ttgagaaaac gagttatgga
     6841 tattacatat cgccctcgcc gaaatcgtcg cacacctgct cttcgtaacc tgatcgcgga
     6901 aacttccttg agcgtggatc aattcattta tccacttttt ctctgtgaag gagaccagca
     6961 aaaagtcgct atcaagtcca tgcccggcca attccgattg agcgcggatt atctgctgaa
     7021 agaaatcagc gaatgtcagt ctttaggaat ccactccttc gctctgttcc cggccatcaa
     7081 tgaaactttg aaaaactccc agggaaagga gtctttcaat ccggcggggc tgcttcccac
     7141 cacggtgaaa aagatcaaag acaagttccc cgattcctgc attatcaccg atgttgcctt
     7201 ggatccttat tcaagcgatg gtcacgatgg aattgtgaaa gacgggcaaa ttctcaacga
     7261 tgagaccatc gaaattttag tgaaaatgtc tttaactcag gcggaggctg gagccgacat
     7321 ggtggctcct tctgacatga tggatggacg cgttggcgcc atccgcaggg ccttagatag
     7381 cgaaggattc acccaaacag gaatcctggc ctactcagca aaatatgctt ccagttttta
     7441 tggccccttt cgtgacgccc tagagtctgc tccgaagttt ggtgataaaa aaacctatca
     7501 aatggacccc agaaattccc gcgaagctct gctcgaagcg gaactcgaca ttcaagaagg
     7561 cgcggacatg atcatggtca aacctgcttt ggcttacctg gatatcattc agctcttgaa
     7621 acaaaacacc accctgccga tcgccgccta caatgtcagc ggagagtacg cgatgattaa
     7681 agcggcagcg caaaatggtt ggctggacgg cgaaaaagcc atgatggaaa gtcttatctc
     7741 tattcgccga gccggcgcgg acattatact gacctacttc gccaaggaag ccgcactggt
     7801 tttgaatcgc tagaataatt catcaaataa ctgtttccag tgcacaaagc gaatgccgtc
     7861 ctttacaaag gctactgggt cccgactcac acaaacgcct aaggcatttg ggaaatcttt
     7921 aacaatttta cgaaagccca cccaatgaga gtcaggcaga ttttggatgg cgtctgtcga
     7981 tttaatttct acaagtaaac gttgagctcc cgggcgctca acaattaaat ctatttctac
     8041 attgttcttg gtcatcaaat acgaaaattt ccaagatttc ttaaaataaa aattcagacg
     8101 aataaattca agaataataa actgttcaaa gagatctcca aattcaaaac tttgctccac
     8161 cagcggtaag gttaaacggt tttgtaaact tcgagtaacg cccgtatcga aaaaataaaa
     8221 ctttggattt tttctttgtc tttcgcgaat agactcatga aaaggctgca acaaaacacc
     8281 cgcaaatgta tcctctaaaa tttgaaaata attttgtacg gttgttgtgt cgacgccaat
     8341 tgattttgca atattgctat agttaataat tttagaattt ccttgggccg cgacttctaa
     8401 aaagtgccta aacggagtca cattcctcac gatttgctca gcaataattt cttctcgcag
     8461 ataagcatca caatatgaat taagataatc tattttatct tcaacttctg cgagagaaaa
     8521 tattttcggc aatgttcccc aacttaatgc agccataata tcgaatgagt cccctaactc
     8581 ctta
//
LOCUS       DATBTE010000168         4998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_89817, whole genome shotgun sequence.
ACCESSION   DATBTE010000168 DATBTE010000000
VERSION     DATBTE010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89817"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      3..96
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(233..1882)
                     /locus_tag="VIG33_06295"
     CDS             complement(233..1882)
                     /locus_tag="VIG33_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY8270481.1"
                     /translation="MRNFITLTLVLALLSACAHNSTKPETAVAGASINATTSAAAVPE
                     NAVPLKDLESFRLKDAEGPQVVDQELEVIPTEINPLVEQWIGYFQGRGRRHMERYLAR
                     STRYEKLMKKVLKDNGLPEDLFYIALIESGFNTQARSHAAAVGYWQFIRGTGKRYGLE
                     INTMVDERKDPVLATQAAAEYFKGLYSVFGSWYLAMASYNVGENRVKREVMNHYTRDF
                     WELARKKRFPKETIHYIPKFIAAKLIAKDLKKYGFDEIDFMTPIEFDHVNLDKPVNLR
                     VMAEKMNINYEDFKALNPKFKGEVAPVKADNKLELRIPPGMTEQATVAANESIVEKAE
                     FIADAGDTEVYRIRRGDSLHAVARKYRTTVAYLRDLNDLPKGKRLKVGMKLYVPDRTP
                     VAPKRNVVVAKKKSSNEKASVAAETSTPGNGRFYTVQSGDTLSSIAKKYAISITQLKR
                     ANKIRRGRLLKVGIRLQIPGGGNSDNPSNDGAKRLPANAENKVALKNKYHVVKKGDNL
                     ASIADRYKVSMMNIKNSNKRLNPSKLFVGAKILIPDANAME"
     gene            complement(1941..4220)
                     /locus_tag="VIG33_06300"
     CDS             complement(1941..4220)
                     /locus_tag="VIG33_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY8270482.1"
                     /translation="MYSTYAFFALLSLFTSVALGASAEPQKVDFTEELKKILATNLYD
                     LDPKVKESKLPSLSRMKYFEIKKRWKECGALGAKVVAANHDIEAWVASSWLKCEIKNT
                     EGSKDKKSLEKPLEWIRKRKNAGNLNNFSNIFSGPWKNPLWQDYVTGSMAILEANRTV
                     FKVNELLDYNDQLPREVRATLLIYAGEFAESKKDQKKALYFYEQSLFFKDSKVARDHL
                     EAMKSALKLKMNMVAPAIEGVLENEGAEATLEEKMSKSLKAGDLMSALKTAVVIQTEY
                     AGSKVAKRLKDKPFEIYQQIFDTSASDEKDVKCFAEIEKIDALSRAEWAQALHRRADY
                     DQALIFSESALKDLHYSSQATLLYWIAGRSAQLIGQYDRAQVHFSKLIEFHAGTEEAA
                     EALLRSGLIHLRLKNFATAKNQFEKLLAQKKDRYDLSGRYWLVRSLENIDKEKERAAV
                     EAKALTDRYPFSYYGLRLTAAANKGEYLWPNMTSPLPENAHELWLTGEQKNSWNRFKN
                     LTKAGWLLEAQSEIQQLPVTKNPWVQYQYAKYMSKSFQFPMAIRWMSDAMDMENSLRS
                     PETLAFVYPKAFSPWIESEAKKYNLNPVLIRSLIRQESAFGIRALSTSNAQGLMQLIP
                     STAQDVARRLGIKKLEFPEDVFRPEINIPLGTFYISMMLEQFSGNVPFALGAYNVGPT
                     KMKVFMEARDEVKNLATKASSNPLDEIWFDELPWTETSFYIKAILRNTLLYKLLDSSK
                     VDWNLVLWQDLHNKKLNLK"
     gene            complement(4234..>4998)
                     /locus_tag="VIG33_06305"
     CDS             complement(4234..>4998)
                     /locus_tag="VIG33_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270483.1"
                     /translation="LLSEAGTHSLMHFNPRGWVRPESVGYLEYWGKNLGPWIVAVIAA
                     LIVFFRQKKWNSLMPTALAFTLFFIFSHVILAPWDWDNIKLIIWCYIFAIMTISDFIW
                     NDREKGVKALVFLCFIPGLMIFLHSLPIYNHGVQWVSERELNKAQVLLRNQNVNEGIL
                     ITPAYEHPALLLGHKLYMGYTGHVWSHGYDFVERERIVNQVYEGAEINFSGKQQPKLV
                     YSGPLEKRRENPLFPPKGLSKIGEALDHELYLVEHK"
BASE COUNT         1364 a         1201 c          990 g         1443 t
ORIGIN      
        1 cttttgcctc aaggcaaacc gccggaaacg tcgggacgca aagctacagg ggcttcagct
       61 cgaaaaacta tgagctatgc cagccagtct gccaaaggta ttgagtcacc cgaggaatta
      121 ggtgagcaaa tggaaggtcg atttctacaa aattttatca cggattggtt tcattcccct
      181 agcctatgag ttattaggat tatttctcat aggcaccttt ttttataagc ccctattcca
      241 tcgcattcgc gtcaggtatc agaatcttag ctcccacaaa caactttgaa ggattgagcc
      301 tcttgtttga attcttaata ttcatcatgg agactttata tctgtctgca atactcgcta
      361 aattatcgcc ttttttcacc acatgatatt tgtttttcaa agccactttg ttttcagcat
      421 tggctggcaa acgtttcgcc ccatcgttgc tggggttatc actattgcca ccaccaggga
      481 tttgtaagcg aattcccacc ttcagcaaac gtccacgacg aatcttattc gcgcgcttta
      541 gctgcgtaat gctaatagcg tatttcttgg cgatagaaga cagcgtgtcg ccggactgca
      601 ctgtgtagaa acgaccattg ccgggcgtag aagtttctgc ggcaacggaa gccttttcgt
      661 tagaagactt tttcttagcc accacaacat tccgcttcgg agctacagga gtccgatcag
      721 gaacataaag tttcattcca acctttagac gctttccttt gggaagatcg tttaaatcac
      781 gaaggtaagc cacggtcgtt ctgtacttac gagcgacggc gtgaaggctg tccccacgac
      841 ggatgcgata aacttcagtg tctccggcat cagcgatgaa ttccgctttt tccacgatac
      901 tttcattagc tgccaccgtg gcttgctccg tcattcctgg ggggatgcga agttcaagtt
      961 tattatcggc ctttaccgga gccacttctc ctttgaattt cggattcaaa gccttgaagt
     1021 cttcatagtt gatattcatt ttctcggcca tcacgcggag attcaccggc ttatctaaat
     1081 tgacgtggtc aaattcgatc ggagtcatga aatctatttc atcaaacccg tattttttaa
     1141 gatcttttgc aatcaacttg gccgcgataa attttgggat gtaatgaatc gtctcttttg
     1201 gaaaacgctt ttttctagcc agctcccaaa aatcacgagt gtaatggttc atgacttctc
     1261 gttttacgcg gttttcaccc acattgtaag aagccatggc caagtaccaa gacccaaaaa
     1321 cactgtaaag gcctttaaag tattctgcgg cggcttgagt tgccaaaacc ggatctttgc
     1381 gctcatccac catggtgttg atctcaagac cataacgttt gccagtgccg cgaataaact
     1441 gccaataccc cacggcagcc gcatggctgc gtgcctgagt gttaaaacca gattcaatca
     1501 aagcaatgta aaacaaatct tcaggaagac cattgtcttt caaaactttt ttcattaatt
     1561 tttcataacg agtggaacgc gctaaataac gttccatatg tctgcgtcca cgcccttgga
     1621 aataaccaat ccattgctca accaagggat ttatttccgt agggatcact tccagttctt
     1681 gatctacaac ctgcggtcct tccgcatctt tcaaacgaaa agattccaga tccttcaggg
     1741 gaactgcatt ttctggtact gcagctgcac ttgtagttgc atttatactt gcacctgcta
     1801 ctgcggtttc tggcttagta gaattatgag cacaagcgct caaaagggcc aggaccaacg
     1861 tcaaggtaat aaaattccgc atgattcctc cgaatactct cttcaagcta caaaaaggca
     1921 ccgggattat caataaaaca tcactttaaa tttaattttt tattatggag gtcttgccaa
     1981 aggactagat tccagtccac ttttgaggaa tctaacaatt tgtagagcaa agtattccgc
     2041 aagatggcct taatataaaa actcgtctcc gtccagggca gctcatcgaa ccaaatctca
     2101 tccaggggat tggaagaagc tttagtggcc agattcttca cttcatcccg tgcttccatg
     2161 aaaaccttca tcttcgtagg ccccacgtta taagccccca gagcgaaagg cacgttgcca
     2221 gagaactgct caagcatcat tgaaatataa aaggtaccaa gaggaatatt aatttccgga
     2281 cggaaaacat cttcgggaaa ttccagtttt ttaattccta aacgacgtgc aacatcctgg
     2341 gccgtggagg ggattagttg cattaaaccc tgggcattgg aggtggataa agctcttatt
     2401 ccaaaggcac tctcttgccg aatgaggctg cgtatcaaaa ccggatttaa attatatttt
     2461 ttagcttcgg actcaatcca tggtgaaaaa gccttgggat acacgaaagc caaagtttct
     2521 ggactgcgca aagagttttc catatccatg gcatcactca tccagcgaat cgccatggga
     2581 aattgaaaac tcttcgacat gtatttcgca tattgatact gaacccaggg atttttagtc
     2641 acgggcaatt gctggatctc tgattgagct tccaacaacc agcctgcttt ggttaaattt
     2701 ttaaagcgat tccaagaatt tttttgttct cccgtcagcc ataattcatg ggcattttct
     2761 ggcaatggcg aggtcatgtt cggccaaaga tattcgcctt tattcgctgc tgccgttaaa
     2821 cgcaagccat aataagaaaa aggataacga tccgttaagg cttttgcctc aactgcggcg
     2881 cgctcttttt ctttgtcaat attttctagg cttcttacca gccaataacg tcctgacaag
     2941 tcataacgat ctttcttttg cgctaaaagt ttctcaaact ggttttttgc tgtcgcaaaa
     3001 tttttcaagc gaagatgaat cagaccggaa cgcaagagag cctcagccgc ttcctctgtc
     3061 cccgcatgaa actcaataag ttttgaaaaa tggacctgtg cgcgatcata ttgtcctatg
     3121 agttgtgcgg atcttcccgc gatccaataa agcaaagtgg cttgagagct gtaatgcaga
     3181 tcttttaaag cactttctga aaaaatcaaa gcctgatcat aatccgcacg ccggtgcaag
     3241 gcttgagccc actcggcccg actgagggca tcgatttttt caatttccgc aaagcactta
     3301 acatcttttt cgtcactcgc actggtatca aatatttgct gataaatttc aaatggttta
     3361 tctttaagac gttttgccac tttactgccc gcgtactcgg tttgaatcac cactgcggtt
     3421 tttaaagcgg acattaaatc accagctttt aaacttttac tcattttttc ttcaagagtc
     3481 gcttcagcgc cttcattctc cagcactccc tcaatggctg gagcgaccat attcattttt
     3541 aatttcaatg cgctcttcat ggcttctaga tgatcacgcg ctaccttgct atctttgaag
     3601 aacaaacttt gttcatagaa atacaaagcc tttttctgat cttttttact ttccgcaaac
     3661 tcccccgcat aaattaacaa agtcgcgcgg acttcacggg gaagttgatc gttgtaatcc
     3721 aaaagttcat tcactttaaa cacggttcta ttggcttcta atatcgccat gctgccggta
     3781 acatagtctt gccacaatgg gtttttccag gggcctgaaa agatattgga aaaattattc
     3841 aaatttccgg catttttgcg cttgcggatc cactcgagtg gtttttccag agacttttta
     3901 tcttttgatc cctctgtgtt tttgatttcg cacttcaacc agctgctggc cacccaggcc
     3961 tcaatatcgt gattggcagc aactactttt gcccccaaag ccccgcactc tttccatctc
     4021 ttcttgatct cgaagtactt catgcgcgaa aggctcggca gcttggactc tttcactttc
     4081 gggtcaagat catacaaatt cgtcgccaaa atctttttca actcttccgt gaaatcgacc
     4141 ttctgaggct ccgcggaggc gcccaaagcg actgaggtaa aaagagacaa aagtgcaaag
     4201 aatgcataag ttgaatacat gcctggtgcg ctcctattta tgttcaacaa gataaagttc
     4261 atgatctaaa gcctctccta tttttgaaag gcctttcggc gggaataggg ggttctctcg
     4321 ccgtttctct agagggccgc tataaacgag tttcggctgc tgttttccag agaagtttat
     4381 ttcagccccc tcataaactt gattcacgat ccgctcacgt tccacaaaat cgtagccatg
     4441 ggaccaaaca tgaccagtat atcccatata taacttatgc cccaacaata aggccggatg
     4501 ttcgtaggca ggagtgataa gaattccttc attcacattt tggtttctta aaaggacctg
     4561 tgctttattg agttctcttt ctgaaaccca ttggacacca tgattatata taggcaaaga
     4621 atgcagaaaa atcataagac caggaatgaa gcacaaaaac accagcgcct tcaccccctt
     4681 ttcgcgatca ttccaaataa agtcagaaat ggtcataata gcgaagatat agcaccaaat
     4741 gatcaactta atattatccc aatcccatgg agccaagata acatgggaaa aaataaaaaa
     4801 caaagtgaag gctaaagcgg tgggcattaa agagttccat tttttttgcc ggaagaagac
     4861 gatcagagcc gcaatcacag ccacaatcca gggtcctaaa ttttttcccc agtactccag
     4921 ataccctaca ctttcaggac gcacccagcc cctgggatta aaatgcatca aagaatgagt
     4981 tccagcttct gaaaggag
//
LOCUS       DATBTE010000169         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2275655, whole genome shotgun sequence.
ACCESSION   DATBTE010000169 DATBTE010000000
VERSION     DATBTE010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2275655"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(460..1302)
                     /locus_tag="VIG33_06310"
     CDS             complement(460..1302)
                     /locus_tag="VIG33_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270484.1"
                     /translation="MPLIKDSNHLEVYLEGRKRRTFVGTLSYNEEQDTFQFQYETKYV
                     KSLKAIPIGPDLSIFKRKHISEKGKLFPSFADRIPSRSNPAYEEYCRSQGIAIDERNP
                     IVLLGTIGRRGPSSFVFEPVVQNSFSQDEIVSFRKTLNLTLNELAIAFDINEQTLQRV
                     ESKKSKDQHTLKRMQIYFEFPEVALWELESNGSKLNHQAYGKLKVYFEKQLDKGSSKK
                     TNTKNKNSKSSLSSGVTVGALCRDLSLYNKNAEVIFGGDESLTFYRVKPRGEDLVQIE
                     FNEI"
     gene            complement(1289..2218)
                     /locus_tag="VIG33_06315"
     CDS             complement(1289..2218)
                     /locus_tag="VIG33_06315"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEY8270485.1"
                     /translation="MTTESTKICLKCLEPIVSEEHKYGLHSDCFIAWFKVSSEHEFVS
                     IKQHSVTSQEPGKKPKENQFNTSFFHGMFKKYSADLESSSFILKMRQSDAPEIPEVEY
                     VCNQIGHALGLPVAEHYLINFMGEQTFVTRNFIKKGGASSNLDHIYKYVKEDQAYSCE
                     VLIEIIKEQTKRPYHVETFIQTILYDSLIANHDRHGRNLGFIVMSSASVLSPIYDNVS
                     YLGLFAGEWLKADLNQPGKVAVLGVENPSMTEYVLEFRRLGYDEVVREFYSKIKPPKV
                     IDLIEKSFCSDLMKQALIKMFKKRYKELEDALN"
BASE COUNT          737 a          485 c          422 g          825 t
ORIGIN      
        1 aaggtcagtg aaaaaggggc tttatcagtt tatggtatgg gacgttttcc tgtaaccctt
       61 tacaaagaac aatggcttaa gttgttaggc atagctgaag agatcaaagc ctttatcgct
      121 gaaaacgatg ataagcttaa aaccaaagaa taggttcaac catcgctttg aatacttgca
      181 ctcaagtgga cagcagcgat gtaagatcaa tgttctcacc ctctaaatcc cctcaaaaaa
      241 cttgaagtca cgttctcaac tctccataca gtaattcctg taaaaaaatg aaatgattag
      301 actgtgggga gaggaagttt atgactaatt catgcgacag ttggaagagt gcagtgctaa
      361 aatgcgttcc ttcctttaca aacgtaaatt aggacgtttc acactcaatt aactcaattg
      421 aaaattgaat cgtcttaggc ttgcgctttt ttacccatgt caaatctcgt taaactcaat
      481 ctgcacaaga tcttctcctc ttggtttaac tctataaaaa gttaaagact catctccacc
      541 aaaaataact tcggcatttt tattatacaa agacaggtcc ctgcataaag cgccaaccgt
      601 gacgccactt gaaagacttg attttgaatt tttatttttt gtattggtct ttttagacga
      661 acccttatcg agctgctttt caaaataaac tttcagcttt ccatatgctt gatggtttag
      721 tttgctcccg ttgctttcaa gttcccatag agccacttcc ggaaactcaa agtatatttg
      781 catccgtttt aaagtatgtt gatctttact tttttttgat tctactcttt gaagagtttg
      841 ctcatttata tcaaatgcaa ttgcaagctc atttagcgtt aaatttaacg tttttctaaa
      901 actcacgatc tcatcttgag aaaaactgtt ctgaacaaca ggttcgaaaa caaaagatga
      961 cggccctctg cggcctatag tgcctagcaa aacaattggg ttccgttcat ctatcgctat
     1021 accctgcgat ctacaatact cttcgtaagc gggatttgac cgagagggaa ttctgtcagc
     1081 aaatgaggga aatagctttc ctttctctga tatgtgtttt cttttaaata tacttagatc
     1141 tggtcctata ggtatggcct ttaaagattt cacatactta gtttcatatt gaaactgaaa
     1201 ggtatcctgt tcctcattat agctaagagt tccaacaaat gtacgtctct tgcggccttc
     1261 taggtagacc tctagatgat tagagtcttt aattaagggc atcttctaac tccttatatc
     1321 tttttttaaa cattttaatc agagcttgtt tcattaaatc tgagcagaaa cttttttcga
     1381 taagatcaat aactttaggg ggttttattt tgctataaaa ttctcttacg acctcgtcgt
     1441 aacccaagcg tctaaactct aggacatatt ccgtcataga aggattctcg accccaagca
     1501 ccgctacttt gccgggttga tttaaatctg cttttaacca ttcccccgca aacaaaccca
     1561 aataggatac attgtcatat attggcgaga gaacacttgc ggaggacata acaataaagc
     1621 ctaggttcct accatgacga tcatgattgg caattaggga atcatataaa attgtctgta
     1681 tgaaggtctc gacgtggtat ggtcttttcg tttgctcttt aattatttca attaaaactt
     1741 cacagctata agcctgatct tcttttacgt acttatagat atgatcaaga tttgaagagg
     1801 ctccgccctt cttaataaaa tttcgcgtta cgaacgtctg ctctcccata aagtttatta
     1861 gatagtgttc agctacgggc aatcctagtg cgtgaccaat ttgattgcac acgtactcta
     1921 cctctggaat ctctggagcg tctgactgcc gcattttaag tatgaaagaa gacgattcca
     1981 agtcagccga atactttttg aacatcccgt gaaaaaagct cgtattaaat tgattttctt
     2041 tcggtttttt cccaggttct tggcttgtaa ctgaatgctg ttttatacta acaaactcat
     2101 gttcagaaga gactttgaac caagcaataa agcagtcact atgaagacca tatttgtgtt
     2161 cttcagaaac aattggctct aaacatttta agcatatttt tgttgattca gtagtcatag
     2221 acctcaattt atcttaacac gtacataggt taatatgatc ttactacgta tatatgtcaa
     2281 ctcaatctta ctacgtatat atgttaatag aattctgagg tgtctatatg attaaaagac
     2341 agaatattta aggtcttcaa ggtattgaga tggcttacga ttctcatttt attagaaatt
     2401 tttataaata atacactact ttgtgtgtaa attaattgct ctcaatttac gtatctatgc
     2461 caagggcca
//
LOCUS       DATBTE010000170         2053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1592744, whole genome shotgun sequence.
ACCESSION   DATBTE010000170 DATBTE010000000
VERSION     DATBTE010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592744"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(400..612)
                     /locus_tag="VIG33_06320"
     CDS             complement(400..612)
                     /locus_tag="VIG33_06320"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HEY8270486.1"
                     /translation="MKSYDRIEFDAKEIIAGMSRLKGARRKPTSIALEEDLLSELKAV
                     AGKKGVPYQVLMRLLITDGLKRLKAS"
     gene            complement(599..952)
                     /locus_tag="VIG33_06325"
     CDS             complement(599..952)
                     /locus_tag="VIG33_06325"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEY8270487.1"
                     /translation="MAQFKFILWLAYWYLQNDLFEFEWDRGNLTKSLDKHGVSPDEVE
                     SVFELKLAVPLGRQVSPPVDEERLNIVGPSSEGRMVSVVFTLRDGRVRPISGRDASRK
                     ERKLYEEVRKAIEKL"
     gene            complement(994..1185)
                     /locus_tag="VIG33_06330"
     CDS             complement(994..1185)
                     /locus_tag="VIG33_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270488.1"
                     /translation="MDSLIKWAIGMALSLAVSGQLKSAAFKMAEMAMDAQKHQLSYGK
                     FSSMLTQTPPSRRQHQSKL"
     gene            complement(1749..>2053)
                     /locus_tag="VIG33_06335"
     CDS             complement(1749..>2053)
                     /locus_tag="VIG33_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270489.1"
                     /translation="IIARLCCFCRFENEKNDPPVDSFEFKYIIPGQELPVLQGKLNRE
                     PEHRYFSITIMGDGIPLNLGSLGYEISLKVGEQIIKKFTKDDALLIQIGSKEETKV"
BASE COUNT          568 a          466 c          405 g          614 t
ORIGIN      
        1 cattttttcg caaaaactga gtctcaaatg aaccgtcagg tggctacctt caactcagct
       61 tgttattgcc tgagttgcac tggagaaaat ttcgggggga gtaaaaagca gtgaaattct
      121 atccattgaa aaaatcctga attgaccatt ccaatataga ttctaatttt gcaaaattta
      181 ggaaagcaca ccttccttta taaatgatct gtttttgtaa aaatgaagac ctcaaaaaaa
      241 atctcaaatc ctttagaatt gctatgggtt acagaaattc cgcaaagaaa agtgtatcga
      301 tgactaatgt caccttggca gggttcaata tattcgagat gagccttttg gacccttccg
      361 atggcaaccc gtcagattgg tgagcttttt agatttttat taagatgctt ttaaacgctt
      421 caacccatca gtgatgagta gtcgcatgag gacctgataa ggtactccct tttttccagc
      481 gactgctttt agctcactta aaagatcctc ttcaagagca atgcttgtcg gttttcggcg
      541 tgcgcccttg aggcggctca ttcctgcaat aatttctttt gcatcaaact ctatgcgatc
      601 atagcttttc aatcgcttta cggacttctt catatagctt cctctcttta cggctggcat
      661 ctctgccgct gataggcctc actcgtccat cacgcaacgt gaatacaacc gaaaccatcc
      721 gcccctctga tgagggcccc acaatgttaa gcctttcctc atcaactggc ggagaaacct
      781 ggcgtcccaa tgggacagca agcttcaatt caaagacgga ctcaacttca tcgggactta
      841 ccccatgttt atcgaggctt ttagtaagat ttccgcgatc ccattcgaat tcgaagagat
      901 cgttttgaag gtaccaatat gcaagccaaa gtataaactt aaactgagcc atcttcggac
      961 actatagtac actatactaa tatttgcaag aaattatagt ttagattggt gttggcgacg
     1021 gctgggtggc gtctgcgtca gcatactgct gaatttacca tagcttagct gatgtttttg
     1081 ggcatccatg gccatctcgg ccattttaaa agctgccgat tttaattgcc ctgatactgc
     1141 caagctcaaa gccattccaa tggcccactt gatcaacgaa tccacccgaa cctcctattt
     1201 taagcgcaaa gccttcaacc tatacagatg atcccgcacg gtagtcttgc ctgcgccaaa
     1261 tcttgtggcg atgtccgcca cagttaaacc ctcatcccag taaagttttg ccagctcgtc
     1321 ctggtcgatg cttggttttt tccactgaac ccgatacact ctctttacag agaactgggt
     1381 tggttcgaga atgtttgcat ggcccctacc aaaccccgct tagagatgcg gtcaaaatct
     1441 cgagattcaa tattcaaaga aatcaataac ttgaaagcaa gaatcacgat gctctgaccc
     1501 ctcgttatgt caccaaatgt ggcgaaataa cgtgaattgc atgcgtttgt agttcgcggc
     1561 tgtagtccgt gattttgcgg actacaggtt ttgtagttct gatgttatcg gtgatggctc
     1621 gcaactatca attgcgagct ttatattcaa acgggttttt ccgtacatga actgcactga
     1681 aatgagttct aaaggcgttg aaaatcttgt gagattgaaa tatcgataaa gataatcggg
     1741 agtaatgcct aaacctttgt ttcttcttta gatcctatct gaatcaaaag ggcatcgtcc
     1801 ttcgtaaatt ttttaataat ctgctcgccg accttgagag aaatctcata tcccaaactt
     1861 cctaagttca aaggaatacc gtctcccatg atagttatgg agaagtagcg atgttcaggt
     1921 tctctgttga gtttaccttg taaaaccgga agctcttgtc cgggaattat atatttgaat
     1981 tcaaaagaat ccacaggggg atcatttttt tcattttcaa atctacaaaa acagcaaagt
     2041 cttgctatta tag
//
LOCUS       DATBTE010000171        12979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1933878, whole genome shotgun sequence.
ACCESSION   DATBTE010000171 DATBTE010000000
VERSION     DATBTE010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 12979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12979
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933878"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1036)
                     /locus_tag="VIG33_06340"
     CDS             complement(35..1036)
                     /locus_tag="VIG33_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8270490.1"
                     /translation="MLANNENPSSDAPLIGIAMATYNPSLSYFLGQISSLKNQTFKNW
                     KCVVVDDNSNDKSYQIILDVIADDPRFQIHRNSSNQGSFKTFERALSLLPEEVQFVCY
                     CDQDDIWIPTKLEIQLRAFKDPSVFLVHTDQSLIDEKNRVFVNSCWELEGRNVLEAST
                     DLLLFRNLITGCTTMFRKEVLKTALPFYPLRPHAEMYHHDMWIAMHACVYGKVLGLKE
                     PLVLYRQHGGNLVGVSSLRRNVKLWELASRARRAFNERRGLREDFLRSLRCFENGAGR
                     DQERKLSYLDNPLALLFRGFGFVLGHPLFFRTWVMLGVGLFSVRLKKEETLNTKRAS"
     gene            complement(1069..2127)
                     /locus_tag="VIG33_06345"
     CDS             complement(1069..2127)
                     /locus_tag="VIG33_06345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8270491.1"
                     /translation="MRVSIIIPTFNRASFLVEAIESALTQDYADLEVVISDNASTDYT
                     SEVARYFFKDPRVKYFKNSENIGMVRNWHKAVFEYATGDWFVILSDDDILTNPYFISQ
                     AVQLIKTSKDVTVVYSNSYVYDEGLRTLTKLKVPFHNIEKGVLVFSKRGTVRPQDFAL
                     CNVLFDRKLSAECGAFLNSNNLSCDTELFLRLCLRGRVGVVSQYSSIYRVHSGNLLKS
                     ASKNTDLVVGSLDSLLKPLLEAEKFQLNTDLIRDFIANSRIKREILVSLLKTSAMSKD
                     KGRALYFELQSLLKEYDYRLLPSSFVFTIMLVTASALTPLFVLRRRALYVLNSLKRQI
                     FGRRVYFGLLKQKVYIIE"
     gene            complement(2117..3427)
                     /locus_tag="VIG33_06350"
     CDS             complement(2117..3427)
                     /locus_tag="VIG33_06350"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEY8270492.1"
                     /translation="MLKRLFSFKGKSWVYSLISLLNVACGLSVTALVWYKYSASKESD
                     ILLLATASISILAQLSLVGVEQVLYFYADERKKHQKDADHFFQLAYTWSLMSGAVFAL
                     VFMGLSKYFLILVASGFSEEARTLAGYLLFCLSPQLVMSPSLHVLRAKWALDEKYGRA
                     YLLSAVSSFILLVCLVVTTLLGVTNLEVFGNLSLSVFLTFLGGFIFYNRKFLIKPSRS
                     DWLKMKELILHSSTVKGANAIHNFLVQALISSVLSQMPTGSISIFQYAKRLADGVFAI
                     TAGPQVMIYHSRCAKAVSNWNKIEMRGNILHFLKTFLSLFFVMAFAVYILTPFALSIV
                     AKSFAVSTIDEIRFVYLGVVLWYLIMGIETLAVGIILATRSSLVLFGVNFTFIFLFFL
                     WSRIHKIENGLELIFTTAGFQIVSFSLFTLSALLIIKRRSLSAG"
     gene            complement(3434..4498)
                     /locus_tag="VIG33_06355"
     CDS             complement(3434..4498)
                     /locus_tag="VIG33_06355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8270493.1"
                     /translation="MKILVLSKYSRKGASSRYRMFQYFPYLQEQGVELTTEALFNDGY
                     LDRLYSKKSRLWSVFKAYIHRLQVLLFARDFDILWIEKELFPWIPWFFERWFLRRLQT
                     PYIVDYDDAIFHQYDTHRAAWVRKLLGTKCDQVMGSSEVVVAGNEYLATRGRRAGAKK
                     VITIPTVVSEKVFMPKEIENPIPIIGWIGSMSTAIYLEKLRPVLFELSQKYSFKFRVV
                     GATVEWSEVPVESLPWSEDQEVVLVQSFDMGVMPLLDSPWERGKCGFKLIQYMACGKA
                     VIADPVGVNSEIVEDGKTGYLVTQQKSWYEALERLLMDAPKKAQMGRYGRERFETQYS
                     LEHWQSFLYKLFQECSLKNR"
     gene            complement(4527..5075)
                     /locus_tag="VIG33_06360"
     CDS             complement(4527..5075)
                     /locus_tag="VIG33_06360"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEY8270494.1"
                     /translation="MNDLFKETAIEGCFTFKNSLFQDQRGSFTKMYSSEIFKSLGLNI
                     PVAEVFFSNSHKNVVRGMHFQTPPHDHAKIVSCISGRVLDVVLDLRKSSPSYGQAIGM
                     ELTALNETTLFIPKGCAHGFYTYEDNSIICYIVETHHHKDSDQGVLWNSFGFKWPGEQ
                     VILSNRDLQFPKFQDFVNPFKG"
     gene            complement(5068..5898)
                     /locus_tag="VIG33_06365"
     CDS             complement(5068..5898)
                     /locus_tag="VIG33_06365"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8270495.1"
                     /translation="MRALLTGSTGFVGQNLLHYLRQQGWQLEIIGRHDSMKDMDQKMA
                     DFKPNVVIHLATLFKAEHQSGDIPSLIQSNITFGTQVVEAMVRHKVLNLVNTGTLWQY
                     YEGQREVPACLYAATKTAFESILRFYSSAHQLSVLNLMLSDSFGPKDPRGKLLPKLLS
                     IAGTEEKLQLSAGQQVIEWTFISDIVEAFEVAAKRLILGQEPKKFVSYTAASGEAYSL
                     RESVSLCEKVLSKKIAIDFGAKPYRQREVMSPQKLDPQLPGWSAKVSFIQGIEACLHE
                     "
     gene            complement(5898..7262)
                     /gene="rfbH"
                     /locus_tag="VIG33_06370"
     CDS             complement(5898..7262)
                     /gene="rfbH"
                     /locus_tag="VIG33_06370"
                     /inference="COORDINATES: protein motif:HMM:NF011936.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein RfbH"
                     /protein_id="HEY8270496.1"
                     /translation="MLPNNVLPNSENEAREHIKNLVADYYHKFHAPKQNEEFIPGTSR
                     VNYGGRVYDEKEMVNLADSMLDFWLTAGRYCDGFEKRFSELLGVKYSLLVNSGSSANL
                     VAFSALTSPLLKDRQIKKGDEVITVAAGFPTTVNPLIQYGAIPVFVDICVEDGTYNID
                     VSYLEAARSEKTKAVMIAHTLGNPFNLEAVSAFCKKHGLWLIEDNCDALGSRYNGKYT
                     GTFGDLATSSFYPPHHLTMGEGGAVYTSNPLLKRTAESFRDWGRDCWCPSGKDNTCKK
                     RFGWNLGELPEGYDHKYVYSHFGYNLKVTEMQASVGLAQLDKLEEFSAKRRKNFKFLR
                     EALTGLSDCFVLPNATPNSDPSWFGFLLTVRPGAAFSRDEIVRHLEDKKIQTRMLFAG
                     NMIRQPMFDQLRGTGAGYRVVGDLKETDRVTTNTFWVGVYPGMKTGALDYTAQTITDF
                     VRSK"
     gene            complement(7249..8352)
                     /gene="rfbG"
                     /locus_tag="VIG33_06375"
     CDS             complement(7249..8352)
                     /gene="rfbG"
                     /locus_tag="VIG33_06375"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HEY8270497.1"
                     /translation="MENLVMSADFWKGKRVFLTGHTGFKGSWMALWLKKLGATVTGFA
                     LSPDTQPSLFSEIEKELGIKSVIGDICDFELLNKTMRESNPEIIFHMAAQPLVRYSYD
                     YPLETFQTNIMGTANLLEAARHLKDLKSIVVITTDKVYENAEWAWSYRENDVLGGHDP
                     YSSSKACAEIVTTAMRKSFFSKPGSPGLATVRAGNVIGGGDWALDRIVPDVMGAFQKS
                     QIVKLRNPKAIRPWQHVLEPLGGYLALGQKLFSEPPQKRASVYSSAWNFGPYADNAVT
                     VGELVTLLSAHWPGGKWEVDQGQHPHEAFYLKLDSSKAKHHLGWHSLLNIQRTAQMTV
                     DWYRHFFNKESSAFDLCNKQIEEYSKELNHVAK"
     gene            complement(8337..9104)
                     /gene="rfbF"
                     /locus_tag="VIG33_06380"
     CDS             complement(8337..9104)
                     /gene="rfbF"
                     /locus_tag="VIG33_06380"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HEY8270498.1"
                     /translation="MKAVLLAGGLGTRISEESVLKPKPMIEIGGKPILWHIMKMYAAH
                     GVNEFIICCGYKAYVIKEYFANYFIHNSDITFDVKNNKMEIHENSAEPWKVTVVDTGE
                     DTMTGGRLKRVARYIGNEDFCFTYGDGVSDVNIKATIEFHKSHGKLSTITAVQPPGRY
                     GALKMAGSKVESFMEKPKGDGGVINGGFFVLNSKVIEYIKEDATIWEKEPMEKLAADG
                     QMQAFVHDGFWQPMDTLRDKKHLEELWTAGKAPWKIW"
     gene            9197..10315
                     /locus_tag="VIG33_06385"
     CDS             9197..10315
                     /locus_tag="VIG33_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8270499.1"
                     /translation="MKIILAVNTLGSFISHRRGLYLKLKESHDVRVILPTSETKNSTQ
                     KEIPEAQLLNIPMSRKGVNPLTELGSIVAYYQQYRKNTPDLVHHFTIKPVIYGTLAAR
                     LAGVPKIVNSITGLGFVFTSNSLKAKILGVLVKNLYRFCFSSPKVRVIFQNTDDRDFF
                     VQQRIIQEDRCFLVEGSGVDVHKFTPSPNSDSVMGPKILLASRLLIEKGIFEFIEALK
                     LLKRKNLQFEVVIAGDIDPGNPGSISESQFNEWKRSGIANFLGFQKDMVSILKSIDIA
                     CLPSYREGLPMALLEAMAAGKPLVTTEAPGCRSTVREQKNGFLVPIKDPQALAKALEK
                     LISNPALREQMGEESRRISVELFSTEKITSEIIKIYSV"
     gene            complement(10330..11595)
                     /locus_tag="VIG33_06390"
     CDS             complement(10330..11595)
                     /locus_tag="VIG33_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY8270500.1"
                     /translation="MKRTDLHQKLQVQYPNKQGYGIEKGPPPSLENVWFVIPPPLSEE
                     APFGLSVSLLRKANEILGSLQKFEALTDFDRLVLGLLVKREALQSSRMEGTFSTIDQL
                     LTPRDQINRKEKSAQASVIAYAHVLEKSFSLASKEGPQIFTEDLLRKLHVEMMSRDPE
                     FRGKAGLFRDEVGKNNYATIGGLNRPENSTYNPVPPKYLRKCLKQNLDWMKDEDSLEM
                     SRAGLGPGLVVRMAKGHWHFEAIHPFADGNGRVGRILMVLYMVSEGLAPLYISGYIEA
                     KKKDYYLSLQAAQMKCDEAPLVHFLCKAIIESYEEAEKTKEALVSLPDSWVKKGNFRQ
                     GSASLRALKVILENPILSVKLLEKRLKISQPAAKRAIDQLCDARILRERTGMGRNRIF
                     AAEEVIELLARPFGESTVIALERAASLLG"
     gene            12090..12956
                     /locus_tag="VIG33_06395"
     CDS             12090..12956
                     /locus_tag="VIG33_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270501.1"
                     /translation="MNITDLGDMPFAVAKKQVVSVSERYSVVTRKGNRYTLTTTQSIP
                     ILAHEWGHVLFYESARVHFEFLEKIDMLDVKLDEIKSKLQIAYKEAMDLEKSANLYPD
                     KDAYRKLMEESQTEYFKLVEESELIKGQLNQINEVFIPYKLRSGPYDEFWGDVISMLY
                     TENPAAIYNTLDMRADDAYISAERRKEIVRRNPVAHINHLSKPESIHTKLREAMEFLW
                     DQYLKKPSVMKRKADIAPAVLKALFSELQWAMSFPLKEDLETEDPLDVGFMKTQNDRL
                     KKALQRELADFK"
BASE COUNT         3886 a         3108 c         2469 g         3516 t
ORIGIN      
        1 agctaacgac aatagcaagc gttggagaca ttgatcaaga agccctcttt gtattcaaag
       61 tttcctcttt ttttaagcga acgctaaaaa gacccacacc tagcatcacc caagttctaa
      121 aaaacagagg atgccccaga acaaagccaa aacccctaaa tagcagagcc agggggttat
      181 ccaaataaga aagtttcctt tcttgatctc tcccggcccc attctcaaaa catcttaagg
      241 agcgaagaaa atcttctcgt aatcctcgcc tttcattgaa agcccgacgg gctctggaag
      301 ccaactccca taattttaca ttacgacgca aactagagac acctactaaa ttaccgccat
      361 gctgacgata taaaaccaag ggttctttta aacctaaaac tttaccataa acacaggcgt
      421 gcatcgcaat ccacatatca tgatggtaca tttccgcatg aggtcttaaa gggtaaaatg
      481 gcaaagctgt cttaagaacc tctttacgaa acatggttgt acaacccgta atcaaatttc
      541 tgaaaagcaa taaatcagtg gaagcttcca gaacattacg cccttccagc tcccaacatg
      601 aattcacaaa gactctgttt ttctcatcaa taagggactg atccgtatgt actaaaaaga
      661 ctgaaggatc cttaaaagcc cgcaactgaa tctcgagttt tgttggaatc caaatgtcat
      721 cttgatcaca atagcagaca aactgaactt cttcgggcaa taaactcaac gcacgctcaa
      781 aagtcttaaa gcttccttgg ttagagctat tacggtggat ctgaaagcgg ggatcatcgg
      841 cgataacatc caatattatc tgataacttt tatcatttga attgtcgtcc accacgacac
      901 atttccaatt cttaaacgtc tgatttttta aagaagagat ttgtcctaaa aaataagaaa
      961 gactgggatt gtacgttgcc attgcaatcc ctatcagcgg agcgtcactc gatggatttt
     1021 cattatttgc caacaatcct accccgtcct ttctttcatg cacggtattt actcaattat
     1081 ataaactttt tgctttaaca acccaaagta aacccgacga ccaaagatct ggcgcttcaa
     1141 agagtttagt acatacagcg ctcttcgcct tagcacaaaa agaggtgtca aagccgacgc
     1201 tgtaactaac ataattgtaa atacaaaact ggaaggaagt aaacggtaat catattcctt
     1261 taataagctc tgcaattcaa aataaagggc acgtcctttg tctttactca ttgccgacgt
     1321 tttcagtaaa ctgaccaaaa tctctctctt aattcgagag ttcgctatga aatctcgaat
     1381 aaggtccgta ttcaactgaa acttttcagc ttcaagcaaa ggctttaaga gtgagtccag
     1441 acttccgacc acaaggtcgg tatttttact tgcagatttt aatagatttc cggagtgaac
     1501 ccggtaaata ctggagtatt ggctgacaac ccccaccctt ccccttaggc aaaggcgcag
     1561 aaaaagctcc gtatcacaag acaagttatt cgaattcaaa aaagccccac actctgcaga
     1621 aagctttcta tcaaaaagaa cattgcataa agcaaagtcc tgcggccgca cggttccccg
     1681 ttttgaaaaa accagaaccc ccttttctat gttatgaaag ggaactttca gcttcgtaag
     1741 agtccttaag ccttcatcat aaacataact atttgaataa acgaccgtta catcctttga
     1801 agtcttaatg agctgcaccg cttgcgagat aaaataggga ttggtcaaaa tatcatcgtc
     1861 agacaagatg acaaaccaat cgccggtcgc atattcaaaa accgccttat gccagttcct
     1921 gaccatgcct atattttcag agtttttgaa atactttacc cgtgggtctt tgaaaaaata
     1981 ccgcgcgact tcagaagtgt agtctgtgga agcattgtca gaaatgacca cttcgagatc
     2041 tgcatagtct tgcgtgagcg cactttcaat agcttccacc aagaaactcg ctctattaaa
     2101 agtaggtata attatactaa cccgcactca agctcctgcg tttaataatc aataaagcag
     2161 ataaggtgaa taagctgaaa ctaacaattt gaaatccggc agttgtgaaa atcaattcta
     2221 aaccattttc gattttatga atccgactcc acaagaaaaa tagaaaaata aaagtgaaat
     2281 tgacaccaaa aagaaccaaa ctgctccgcg tcgcaaggat aatccccacc gcaagagttt
     2341 ctattcccat aatcagatac caaagaacaa ctcccaaata aacgaagcgg atttcatcaa
     2401 tcgttgagac cgcaaaactt ttagcaacaa tcgacagagc aaaaggcgtc aaaatataga
     2461 cggcaaaagc catgacaaaa aataagctta aaaaggtctt taaaaaatgg agaatgttcc
     2521 cgcgcatttc tattttattc caattcgaca ctgcctttgc gcagcgcgaa tgatagatca
     2581 taacctgagg acctgcggta atggcaaaga ccccgtctgc gagtcttttt gcatactgaa
     2641 aaatggaaat agaccccgtc ggcatctgcg aaagcacgct gctaatcagc gcctgaacta
     2701 gaaaattatg aatcgcattg gcccccttta cggtcgaact atggaggatc aattccttca
     2761 tcttgagcca atctgaacga gagggtttta ttaagaattt tcgattgtaa aatataaatc
     2821 cgccaagaaa cgtcaaaaaa acagaaagag acaaattacc gaaaacttct agattcgtga
     2881 ctcccaaaag ggtcgtgacc accaggcaaa ccaataatat aaaagagctc acggcagaga
     2941 gcagataagc tcggccatat ttttcatcca aggcccattt tgcccttaaa acatgcaaag
     3001 aagggctcat caccagttga ggggaaagac aaaagagtaa gtatcccgca agagttctgg
     3061 cctcctccga aaatcctgag gcaaccagga ttaaaaaata ttttgaaagt cccatgaaga
     3121 ccagagcaaa aaccgctccg ctcatgagag accacgtata tgcaagttgg aaaaaatgat
     3181 ccgcgtcctt ttgatgtttt tttcgctcgt ccgcataaaa atacagaact tgttccacac
     3241 cgaccaaaga caattgcgcc agaatggaga tcgaagcggt tgccagcaat aaaatgtcag
     3301 actctttgct agcagaatat ttataccaaa ccaaggccgt tactgaaagt ccacaggcca
     3361 catttaacaa tgagatcagg ctgtagaccc aagactttcc tttaaatgaa aataaccttt
     3421 tcagcatcag accctatcta ttttttagag agcattcttg aaataattta tataaaaatg
     3481 actgccaatg ctcgagtgaa tactgagtct caaaccgctc acggccgtac cgccccattt
     3541 gcgctttttt aggcgcgtcc attaaaagcc tttcaagagc ttcgtaccag ctcttctgtt
     3601 gagtcactaa atatcccgtc ttcccgtctt caacaatctc ggaattaact ccgaccggat
     3661 cggctatgac ggcttttcca caggccatat attgaatcaa cttgaagcca cacttacccc
     3721 gttcccaagg agaatccaaa aggggcataa cccccatatc aaaagactgc accagaacca
     3781 cctcctgatc ctcactccaa ggaagggatt ccacgggcac ctcactccac tcaaccgtgg
     3841 ctccgacaac tcgaaattta aaagagtatt tctgactgag ttcaaaaaga acgggcctca
     3901 gtttttcaag ataaatagct gtggacatag atccaatcca tccgataatc ggaattggat
     3961 tttcaatctc tttcggcata aaaacttttt cagaaaccac cgtgggaatg gtgatcactt
     4021 ttttagcacc cgctctgcgc cctcgagttg caagatactc atttccggcc accacaacct
     4081 cagaagagcc catcacctga tcgcactttg tacctaacaa cttccgcacc caagcagcac
     4141 gatgagtgtc atactgatgg aaaatagcat catcataatc cacaatataa ggagtttgga
     4201 gccttcttaa aaaccatctt tcaaaaaacc acggaatcca ggggaaaagc tctttttcaa
     4261 tccataagat atcaaagtcc cgggcaaaca gaagaacctg aaggcgatga atataagctt
     4321 tgaatacact ccaaagacgg ctctttttag aatacaaacg atctaaatac ccatcattaa
     4381 aaagggcttc cgtagtcagt tccaccccct gctcttgaag atacgggaag tactgaaaca
     4441 ttctataacg gcttgaagcc cccttgcgag agtatttgct taaaactagg attttcactc
     4501 aagcctcttc atcaactctc ttgctttcag cctttaaaag gattcacaaa gtcttgaaat
     4561 ttcggaaatt gcagatctcg attcgaaaga ataacttgct caccgggcca tttgaaacca
     4621 aatgaattcc aaagcacccc ttggtcagaa tccttgtggt gatgggtttc aactatataa
     4681 caaattatac tattatcttc ataagtataa aaaccatggg cgcagccttt tgggataaat
     4741 aaagtggttt cgttcaaagc cgttaactcc atcccaatgg cttggccata gcttggagag
     4801 cttttgcgca aatctaatac cacatccaaa actcttccgg aaatacaact cacaatcttt
     4861 gcatgatcat gagggggcgt ttgaaaatgc attccccgca ctacattttt atgggaattt
     4921 gagaaaaaca cttcagccac cggaatatta agtcccaagg atttaaatat ttcacttgaa
     4981 tacattttcg taaaagaacc gcgttgatct tgaaaaagac tgtttttaaa ggtgaaacat
     5041 ccctcgatag cggtttcttt aaataaatca ttcatgaagg caagcctcga tcccttgaat
     5101 gaaggacacc tttgcggacc atcccggcag ttggggatct aatttctgtg gactcataac
     5161 ctcacgttga cgataaggtt tcgccccaaa atcaatcgca attttcttgc tgaggacctt
     5221 ttcacaaaga ctcacgcttt cacgcagact ataggcttcc cctgaggcgg cggtgtaact
     5281 tacgaatttt ttcggttctt ggcccaaaat cagtcttttc gcggcgactt caaaagcctc
     5341 tacgatatcg ctgataaaag tccactcgat cacttgttga cctgcagaga gttgaagttt
     5401 ctcctctgtc cccgcaatac ttaaaagttt cgggagcaat ttacccctcg gatctttggg
     5461 accaaaacta tccgaaagca tcaggttcag gacactcaac tgatgggcgg aactataaaa
     5521 cctcaaaata ctctcgaagg ctgtttttgt cgccgcatac aaacaagcgg gcacttctcg
     5581 ttgcccctca tagtattgcc ataaggttcc cgtattcacc aagttcaaga ccttatgacg
     5641 aaccatggcc tccaccactt gagtgccaaa agtaatatta ctttggatta aagacggaat
     5701 atcccctgac tggtgctccg ctttaaaaag cgttgccaag tggatcacca cattcggttt
     5761 aaagtccgcc attttttgat ccatgtcttt catgctgtcg tggcgaccaa tgatttctag
     5821 ctgccaccct tgttgacgca aatagtgcaa aagattttga ccaacaaagc cagtggatcc
     5881 cgtcagaagc gcacgcatta tttgcttctc acaaaatcag taattgtttg agcagtgtaa
     5941 tctaaggccc cggtcttcat tccgggatag acgcccaccc agaaggtatt cgtggtcacg
     6001 cgatccgttt ccttaaggtc tccgacaact ctgtaacctg cacccgttcc gcgcagttga
     6061 tcaaacatag gctgacgaat catattcccc gcaaataaca ttctcgtttg aatcttctta
     6121 tcttccaaat gacggacaat ttcatcccgc gaaaaggccg cccctgggcg caccgttaac
     6181 aagaatccaa accaactagg atctgagttc ggagtcgcat tgggcaatac aaagcaatca
     6241 gacaaacccg ttaaagcctc gcggagaaat ttaaagtttt ttcttcgctt cgcactaaac
     6301 tcctccaact tatccaattg agccaaacca acggaggcct gcatctcagt cactttcaaa
     6361 ttataaccga aatgactgta gacatattta tgatcatagc cttctggcaa ttcacctaaa
     6421 ttccagccaa aacgtttttt acaagtattg tctttcccac tggggcacca acaatcacga
     6481 ccccaatctc ggaaagactc cgcggttctt tttaggagcg gatttgaagt ataaaccgcg
     6541 ccgccctcac ccatggtcag gtgatgagga ggataaaaac tgctggtcgc caaatcacca
     6601 aaggttccgg tatacttgcc attataacga gaacccaggg catcacaatt atcttctata
     6661 agccaaagac cgtgtttctt acagaaagca ctcaccgcct ccagattaaa ggggttgccg
     6721 agagtgtggg cgatcatcac cgccttggtc ttttcacttc gcgccgcctc aagataggaa
     6781 acgtcaatat tgtaggtccc atcttctaca cagatatcga caaaaacagg aatggcacca
     6841 tactgaatca gcggatttac cgtggtggga aagccggcgg ccacagtgat aacttcatcg
     6901 ccttttttaa tctgtcgatc tttcaataac ggagacgtga gcgcgctaaa agccactaga
     6961 tttgccgaag atccggaatt caccagaaga gaatatttta ctcctaagag ttcggaaaaa
     7021 cgcttctcga aaccatcgca gtaacgaccc gctgttaacc aaaagtcgag catggaatcc
     7081 gccagattga ccatctcctt ttcatcgtaa acccgtcctc cgtaattgac tcgggaagta
     7141 ccgggaataa actcttcgtt ctgtttcggc gcatgaaatt tatgatagta gtcagcaact
     7201 agatttttaa tatgttcgcg ggcttcattt tcgctatttg gcaacacatt atttggcaac
     7261 atgattcaat tccttagagt attcctcaat ctgtttatta cataaatcaa aggcactgct
     7321 ctctttgtta aaaaagtggc gataccagtc aacggtcatt tgtgcggtcc tttgaatatt
     7381 taaaagcgaa tgccaaccaa gatggtgctt cgcttttgaa ctgtccaatt tcaaatagaa
     7441 agcttcatgg ggatgttgtc cttgatcgac ctcccacttt ccccccggcc aatgagctga
     7501 gagcaaagtt acaagctcac ccacggtcac cgcattatcg gcatagggac caaagttcca
     7561 agcggaagaa tagacactcg cgcgtttctg tgggggttct gaaaataatt tctgtcctaa
     7621 agctaaataa ccccctaaag gctcaaggac atgctgccag ggacgaatcg cttttggatt
     7681 cctcaacttt actatctgtg atttctgaaa ggcccccatc acatcgggaa cgatgcgatc
     7741 caaggcccaa tctccaccgc caataacatt tcccgctctc acggtcgcca gcccgggact
     7801 tccaggcttt gagaagaaag atttccgcat ggccgtggtt acaatttccg cgcaggcttt
     7861 actgctagag taaggatcgt ggcccccgag cacatcattt tctcgataag accaggccca
     7921 ctcagcattt tcataaactt tatctgtggt gataacgacg atggatttta aatctttcaa
     7981 atgcctcgca gcttctaaaa gattggctgt gcccatgata ttggtctgaa aggtttccag
     8041 cggataatca tatgaatagc gaaccaaggg ctgagccgcc atatggaaaa taatttccgg
     8101 attggattcc cgcattgttt tgtttagtaa ttcaaaatca cagatatcac caataacaga
     8161 tttgataccc aattcctttt caatctcaga aaaaaggctg ggctgcgtat cgggacttaa
     8221 ggcaaatcct gtgacggtcg ctccgagctt tttgagccac aaagccatcc aactgccttt
     8281 aaatccagtg tgccccgtga ggaaaactcg tttgcccttc caaaaatcag cactcattac
     8341 caaattttcc atggagcctt tccagcagtc cagagttctt cgagatgctt tttatctctt
     8401 aaagtatcca tcggttgcca aaaaccatcg tggacgaagg cctgcatttg tccatcggcc
     8461 gcgagttttt ccatgggctc tttttcccaa atggttgcgt cttctttgat gtattcaatc
     8521 actttactgt tcaagacgaa aaatccgccg ttgataacgc cgccgtcgcc tttgggtttt
     8581 tccataaagc tttcaacttt agatcctgcc attttcaaag caccgtaacg ccctgggggc
     8641 tgaaccgcag tgatcgtgga taattttccg tgagatttat gaaactcaat ggtggcttta
     8701 atatttacgt cactgacgcc atcgccatag gtaaaacaaa agtcttcatt gccaatgtat
     8761 cgggccaccc gcttcaagcg cccgcccgtc atggtgtctt caccggtgtc gacgacagtg
     8821 accttccaag gttccgcgct attttcatgg atttccattt tattattttt aacgtcaaaa
     8881 gtgatatctg aattgtgaat aaagtagttc gcaaaatatt ctttaatgac ataggcctta
     8941 taaccgcagc aaataataaa ctcgtttacg ccatgggcgg catacatttt catgatgtgc
     9001 caaaggatcg gtttgccgcc gatttcaatc atgggcttcg gcttcaaaac actttcttca
     9061 ctaatgcggg tccccaagcc accggcgaga agcactgcct tcatttatcc tcctcaaaat
     9121 tatgccaacc agatatttga tcggattatt atctttaatt gacgcaaaag ctatctcaaa
     9181 ttacacctaa agccctatga aaatcattct tgcagtaaac actttgggct cttttatttc
     9241 tcatcgacgc ggcctgtatt tgaagctcaa agaaagtcac gatgttcgag tgattttgcc
     9301 gacttctgag accaaaaact ccactcaaaa agagattcca gaggcgcaac ttttaaatat
     9361 tcctatgagt cgaaaagggg tcaacccctt gaccgaactt ggctcaattg tggcctatta
     9421 ccaacagtac cgcaaaaaca ctccggactt ggttcatcat tttactatca aaccagttat
     9481 ttatggcact cttgccgcaa gactcgcggg cgtaccaaaa attgtgaact ccattaccgg
     9541 ccttgggttc gtttttacct cgaactcctt aaaagctaaa atattaggcg ttctcgtgaa
     9601 aaatctttat cgcttttgct tttccagccc caaggtgcgg gttatttttc aaaataccga
     9661 tgatagggat tttttcgttc agcagcgaat cattcaagaa gatcgctgtt ttctcgtgga
     9721 aggctcgggc gtcgatgtcc ataaatttac gcccagcccc aatagtgaca gcgtcatggg
     9781 gccaaaaata cttctcgcct cccgattgct gattgaaaaa gggatcttcg agtttattga
     9841 agctttaaaa ttgcttaaac ggaaaaatct tcagttcgag gtggtcatcg ccggagatat
     9901 tgatcccgga aatcctggct ctatttctga atcccaattc aatgaatgga aacgctctgg
     9961 aattgcaaat tttttgggat ttcaaaaaga catggtttcg attttaaaat ccatcgatat
    10021 cgcctgcctg ccttcttacc gggaaggtct gcccatggct cttcttgagg ctatggccgc
    10081 aggcaaaccc ctggtgacca cggaggcccc tggctgtcgc tccaccgtca gagaacaaaa
    10141 aaatggtttt ctggtaccga ttaaagaccc gcaagccctc gccaaggccc tggaaaaatt
    10201 aatttccaac cccgcccttc gtgagcaaat gggagaggaa tctcgaagaa tttctgtcga
    10261 gttgttctcg actgaaaaaa tcacctctga aattattaaa atatattctg tttgaccgcc
    10321 ctatgaactt tatcccaata aggaagctgc acgttcaagg gcaattacag tgctctcccc
    10381 aaaagggcga gccaacagct caatgacttc ttccgcagcg aaaatacgat ttctacccat
    10441 tcctgtacgc tctctcaaaa ttcttgcatc gcaaagttgg tctatcgctc tttttgcagc
    10501 cggctgactg atcttaagcc gtttttctaa tagtttcacc gaaagtattg gattctcaag
    10561 tataactttt aatgcccgaa gcgaggcaga tccttgacgg aaattacctt tctttaccca
    10621 gctatcagga aggctgacca aggcctcttt tgttttttcc gcttcctcgt aagattcaat
    10681 aatggcttta cagaggaaat gaacaagagg agcttcatca cacttcattt gtgcagcctg
    10741 aagacttaaa tagtaatctt tcttttttgc ttcgatataa cctgaaatat aaagcggcgc
    10801 gagcccctca ctcaccatat ataacaccat tagaattctt cctacacgac cattgccatc
    10861 agcgaatgga tgtatggctt caaaatgcca atggcctttt gccatcctta ccactaaacc
    10921 tggccctaga cctgcccgac tcatttccag gctgtcttca tctttcatcc aatctaagtt
    10981 ttgcttgagg catttccgca aatattttgg aggaacggga ttataagttg aattttccgg
    11041 cctattcagg cctcctattg tcgcgtaatt atttttacca acctcatcac ggaagagccc
    11101 ggcttttccg cgaaactccg gatccctcga catcatctcg acatgcagtt tacgaagtaa
    11161 atcctcggta aatatttgcg gaccttcttt tgaggcaagg ctaaaagatt tttccagaac
    11221 atgcgcatag gcaatgactg aagcttgagc tgacttttcc ttcctattta tctgatctct
    11281 aggcgtcaat agttgatcaa tggtggaaaa ggttccttcc atacgagagg actgaagagc
    11341 ctcacgttta actaaaagtc ctaaaaccaa acgatcgaag tctgtaagag cctcgaattt
    11401 ctggagagac cccagaatct cattggcctt acgtaataaa gaaacactca ggccaaaagg
    11461 cgcttcttcg ctcagtggcg gcggtattac aaaccataca ttttccaaac ttggaggcgg
    11521 acctttttct attccgtaac cttgcttgtt agggtattga acttgaagct tttggtgaag
    11581 gtccgtgcgt ttcataaagt tatttataac acactatata taacgttgat atattaaagt
    11641 tatatataag acggcccata taacttttca ggttttcaaa aaagcttccc gacgcacaac
    11701 gatcgttgca tcatttttaa ttttaaaaaa ttaaacattg acagctgtct ataaaaatat
    11761 ccccttaaag acggctgaaa atcctcacct ctggaccgcg atgtgacaca actatcgagc
    11821 aaccctgtat ccttcaccgc taacttgaac acaatgtcgt atgttcttta gatgttcttt
    11881 agaatcttaa tgcctctcaa cttgaagcgg cccaaaattg tagtgaggtc ttttcaaacc
    11941 cctccatgga agttcgactg acactttatg aaaatactgg caagccttta aagttggtaa
    12001 atcggaaaac tcccgaaaaa tttgtttcaa tcattcaaga aattaatacg aaaattgcta
    12061 aaaatcttat tttgcctccc tttgttgatt tgaatatcac cgacctcggc gatatgcctt
    12121 ttgctgtggc caaaaagcaa gttgtaagcg taagtgaacg ctatagcgtg gtgactagaa
    12181 aagggaaccg ctatactttg accaccactc aaagcattcc aattcttgcc catgagtggg
    12241 gccatgtgct tttctacgaa agtgctcgtg ttcattttga atttttagaa aaaattgaca
    12301 tgcttgatgt taagttggac gaaataaaga gcaaattaca aattgcatac aaagaagcaa
    12361 tggacttgga aaaatcagca aacctttatc cggacaaaga tgcttatcgc aagctcatgg
    12421 aagaatcaca gactgaatat ttcaaattag ttgaggaatc ggagcttatt aagggtcagc
    12481 tgaaccaaat caacgaagtt tttattcctt ataaacttcg aagcggtccc tatgatgagt
    12541 tttggggcga tgttatttcc atgctatata ctgaaaatcc cgcagccata tataacacat
    12601 tagatatgcg ggccgatgat gcgtatattt cggccgaacg tcgtaaagaa atagtacgac
    12661 gaaatccagt tgcccatatt aatcatttga gcaaaccgga atctatccat actaaactgc
    12721 gcgaagccat ggaattttta tgggatcagt atttgaaaaa accttcagtc atgaaacgca
    12781 aggcggatat agcgccagct gtgttaaaag ctttattcag tgagcttcag tgggcgatga
    12841 gttttccact taaagaagat ttggaaacgg aggatccact cgacgtaggt tttatgaaaa
    12901 cccaaaatga tcgattgaaa aaagcactgc agcgcgaact tgctgacttt aaatgatccg
    12961 taaattattt caatagatt
//
LOCUS       DATBTE010000172        12663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2071681, whole genome shotgun sequence.
ACCESSION   DATBTE010000172 DATBTE010000000
VERSION     DATBTE010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 12663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12663
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2071681"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..794)
                     /locus_tag="VIG33_06400"
     CDS             complement(219..794)
                     /locus_tag="VIG33_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY8270502.1"
                     /translation="MNFLFLFLFLGLPNFALSISKDQVPSKSIYQLEQSWTTQENKSL
                     VLKDLSGLPAVVTMTFSSCPGACPLMISDMKFFDSQLNKKEREQIQYFTFSIDPVRDT
                     AEVLQKYHKKMHLDKRWKLLTSDKDQVREMAAILGFSYKEVGGGDFTHSTSLYLLSAK
                     GEILARKERNSDWKEFLDKFRGQLKAAPVEK"
     gene            complement(791..1561)
                     /locus_tag="VIG33_06405"
     CDS             complement(791..1561)
                     /locus_tag="VIG33_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEY8270503.1"
                     /translation="MKTIASLILVMFFNGSWTSALSADQILIPAGKFKPFFKELKDQD
                     IEIHPFYLDRYPVTNADFNEFILKNPSFKKNKIISLYVDQRYLQDWKSEVLSGDELKT
                     MGANPVTYVSWFVAKKYCQTLGKRLPTIAEWEYASDPENPDILKELLEWYAKTGDQPI
                     EKVGLRKPNKFGVHDMHGLIWEWIEDFSSVMISSDSRSKGDRTDGFFCGGGSVSSLDS
                     KSYASFMRYGFRSGLKGDYCMRNLGFRCASDLKKEKSQ"
     gene            complement(1566..2990)
                     /gene="nirK"
                     /locus_tag="VIG33_06410"
     CDS             complement(1566..2990)
                     /gene="nirK"
                     /locus_tag="VIG33_06410"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HEY8270504.1"
                     /translation="MKKYFITTCLLILSSVSLAKSAVNSKYADPKNFPEETAILTTAP
                     KVPPPITRRTPARVKVDLETKEVTGRLADGVEYNFWTFGGTVPGPMIRIRVGDYVEFN
                     LHNHPSSKMPHNIDLHAVTGQGGGAEGSMTIPGHSSKFSFRALNAGLYVYHCATAPVG
                     MHIANDMYGLIYVEPEQGLPKVDKEFYVVQSDFYTKGKYGEKGLQAFDMEKAIKEQPD
                     YVVFNGSVGALAGDNSVNAKVGEKVRIFFGTGGPNIASNFHVIGEIFDKVYVEGGTKV
                     VQENVQTTVVPPGGSVIVDFKIDIPSTYILVDHAIFRAFNKGALGMLKVVGDKDPMIY
                     TGKQSDTVYLPEGGATQTMPAGKVPEKIFAKNLEERIAAGKIVFTQNCAACHQADGKG
                     LSGAFPPLAKSDYLMADKKRAISTIVNGMQGKAVINGVEYNGVMPALGLNDEDIANVL
                     SYVRNSWGNKSDEVTPSEVKSVRK"
     gene            complement(3003..3371)
                     /locus_tag="VIG33_06415"
     CDS             complement(3003..3371)
                     /locus_tag="VIG33_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEY8270505.1"
                     /translation="MNRLMKRREFFKNMAKISGVAIAAPALISAFTGKMAFAEEGRRK
                     KSGDAGSQLVDPSEATAKAVGYVEESTKSPKAAGNKCANCILFVKPEKKNGKEVGSCA
                     IFPNKFVVANGYCNSWAKKA"
     gene            3543..4199
                     /locus_tag="VIG33_06420"
     CDS             3543..4199
                     /locus_tag="VIG33_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8270506.1"
                     /translation="MSKLPPAPTEFCPICSSHEEKVTDQILQIVQKKSYAKNCVIFEQ
                     EQEASGIYLIQKGSVKISRISPLGKEILIEILSAGKTFGASGLFGQGRHADTATTSEN
                     CELFVLPKKEFKEILSAHPELYHSVIQSLCEWMDRLNNIIENINTPSARERVASYLKR
                     TQQDQNNILIHLNGKKHEVAMMLGLRPETFSRALAELEAEGIIKMNHKQIQILLPVKL
                     "
     gene            4303..4851
                     /locus_tag="VIG33_06425"
     CDS             4303..4851
                     /locus_tag="VIG33_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270507.1"
                     /translation="MLKLKSDTIKIHEPFAEYLGNTPEIIDFEISLLDVAKLSGHLCP
                     SVAGAFLLTRKAVELLYPDTKVCIRGQMEVHLPGAPDEGPLGPMANVISYITGAWSET
                     GFGGLGGTRYLRKDLLHFASPLCKKREFVFIRLDNKKTASVFFNPKPILQKLTNSEKT
                     FQESWQANVEAILNSSDAIESF"
     gene            complement(4866..6683)
                     /locus_tag="VIG33_06430"
     CDS             complement(4866..6683)
                     /locus_tag="VIG33_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8270508.1"
                     /translation="MSFDEQLFGWIHRKYRDWKNPAIDAPGVVHLEGLKGVLQTLARA
                     YGGRAYEIHNAEKWGGIQGLHLYLPAKMSLSPDEKKNIQAYVYRTLISVGFDLENIYF
                     PANPEVNEQLALKTYFLNLRRVSLRLQAELPQFVQLEEDLIQTLSPDPWQALWENSLT
                     IENLNKTHYQNWPLLWGLVYRRQAEGEAVLDLPDSLRAQESAFPQGTELQGKIREKAE
                     VKKIPLKKQEDSPLIHIFEKVKTAEDYNGGFKNMDGSDEILEHEEAIDELEMKSVIRT
                     EEGAASLYRADIHLNINVPEIKDEAISSPQDGLQTYLYPEWDYQKKLLKPDWCTLRES
                     VIKGEFLREDFQEARLGLRKKYKKDIERLRQQLEKQFSMSRWRGGQKDGPEIDLSKMV
                     DRYSFLQAGQTPPENLYMRRERRDCDWASYILIDTSLSTDSWVNNKRVLNFEREAIYL
                     LGEAMGLYPEQIQIGAFYSHTRQDCRLEVVKAWDESWIEGLKKLWSLKPRAYTRIGPA
                     IRHASAQLEKVKARKKFLILLSDGKPTDFDRYEGHYGIEDVKHALRQATQRRIQVHAL
                     TVEEQAKSTLAQMFLYSNYQIVKQPEDLVDHLLKIYLQR"
     gene            complement(6686..7453)
                     /locus_tag="VIG33_06435"
     CDS             complement(6686..7453)
                     /locus_tag="VIG33_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HEY8270509.1"
                     /translation="MIFYKAIRNEVEVFKHAYSNQLPLLIKGPTGCGKSRFVEKMAEE
                     MGRPLISVACHDETSAIDLMGRYLVQGGDTVWQDGPVTRAVREGAILYLDEIAEARED
                     VVVVLHPLTDHRRHLYLERRNELLQATKEFMLVVSYNPGYQKAFKEMKPSTRQRFLGL
                     SFNYPHKELETEIVQAESTCEKSVAGKLVFFGQKVRNLQELGLTETVSTRLLIGAGKL
                     IQSGLSPRQACDVAIVQALTDDGEIAEGLNNLAALVF"
     gene            complement(7440..8777)
                     /locus_tag="VIG33_06440"
     CDS             complement(7440..8777)
                     /locus_tag="VIG33_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY8270510.1"
                     /translation="MKYQTQKIAYYFFSVCMLLLGLQLVYGFIMAFDHMGIEGLHRWI
                     PFHVARATHTNLLLMWLLTGFMGAAYYIVPEEADSELVHPKLAILQLVAFVVVGVTAV
                     VGFHLNWWEGRKFLEIPRPLDYFVVVDVLLFLYLIGMTIWKGKRKTTTSMVLFFGLLS
                     AALLYLPGMISTTNQTMDSYWRWWVVHLWVEGVWELIMGAILSYLLIKLTGIDREIIE
                     KWLYIIVGFTFLSGILGTGHHYYYIGTPKYWLMIGGIFSALEPLAFLGMAIYAITLAK
                     KGGRQHPNKTALSWTIGCSVMSCVGAGFLGFAHTLPSVNMYTHGTLVTAMHGHMAFWG
                     AYAMLILAIMTYAMPSLTGRKLHDSKASVFAFWTSNVGMIAMTVAFGIAGVTQVYLER
                     RMGMDFLTTQKELEVHFFGLILAAGLFTSGIIAFIYNFVKYGGPHGEVGRDIL"
     gene            complement(8787..9449)
                     /locus_tag="VIG33_06445"
     CDS             complement(8787..9449)
                     /locus_tag="VIG33_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8270511.1"
                     /translation="MLSKSAAKSFFLIGTAVCGGAFVLLTLDTIKQVPARTNEENITV
                     SVHRGKDLWDKSNCMGCHTLLGEGAYYAPELTKVFQRRGADFIKKMLQDPESMYPGQR
                     KMQKYNFTEEEREDLVSFLKWVGEIDSNGFPAKPPLAVAMTLPHPVIFDQMCIACHSL
                     GGRGGQVGPALDGVGSRRDKAFLMAWLKDPTQIKADSKMPKLPLQDLDIQALADFLSQ
                     NK"
     gene            complement(9665..11500)
                     /gene="nrdD"
                     /locus_tag="VIG33_06450"
     CDS             complement(9665..11500)
                     /gene="nrdD"
                     /locus_tag="VIG33_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEY8270512.1"
                     /translation="MLTRLTDEQIQQKINFIESYLHAKNAADGSKLDANANVAHKNIA
                     TLEAEINKDINIQVNRQLVANKISEIFDKELACEYVRQIQEHEIYVHDETSLKPYCAS
                     ISMYPLLIDGLKKLGGESEAPKHLESFCGTFVNLVFAISSQFAGAVATVEWLMYFDHF
                     ASKDYGADYLNTHTKIIDNHLQHVVYAVNQPAAARGYQSVFWNISVYDHYYFKSLFGD
                     FVFPDGGVPDWGRVSKLQNHFMTWFNSERKKALLTFPVVTAAMLVDSQKPRDHAFASM
                     CAQELSEGNSFFMYMSESADSLASCCRLRNEISDKTFSYSLGAGGVATGSINVITMNM
                     NRITQKKLDLQECVEKVQKYQVAYRRLMEEYFKAGLLTAYSAGFISLDKQFLTIGING
                     MVEAAESQGLLARNEASYKKFVREKLKIIFETNKRAKNKFGYMFNTEFVPAENLGIKN
                     AQWDRKEGLMVPRDCYNSYFYVVEDDSVTPIDKIQMHGQEMMQYLDGGSALHLNLEEK
                     LTKEAYYKLLCAAARAGCNYWCVNVKLTICNDCDHIDKRTLYQCSRCKSEDVDHATRV
                     IGYLKRISSFSSGRQKEAVRRFYHDPQGPKIIPTRQGASILESVI"
     gene            complement(11518..11970)
                     /gene="nrdG"
                     /locus_tag="VIG33_06455"
     CDS             complement(11518..11970)
                     /gene="nrdG"
                     /locus_tag="VIG33_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEY8270513.1"
                     /translation="MNIYKYDIVFQEVPDEISLAFYVCGCPLQCQGCHSPELWTQKNG
                     KDLSSEFYRHLLERYQGYVSCVLFMGGEWHLAQLNAFLKIARKLKYKTALYTGLEKIP
                     KVLEQNLDFAKLGPWNPALGGLASPMTNQIFLDLNKKQILNHLFQPPS"
     gene            12155..>12663
                     /locus_tag="VIG33_06460"
     CDS             12155..>12663
                     /locus_tag="VIG33_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8270514.1"
                     /translation="MFDKIKTIQSLELFQDVKQEDLVVIEKLAECLHIKNNETLFHEK
                     DPQKNVYVIVYGSFKILSKDQQDNVVIFNFLGRNEVLGISMARLANPRFPVSAVANEE
                     SCVLKISVADFNKLFLKNPLLESRIQLQLAQRFLDFQEDRCLRKSLASQRLAEFILRT
                     LDRQPQSCR"
BASE COUNT         3631 a         3058 c         2457 g         3517 t
ORIGIN      
        1 cgatgccgca gccatggacg taagccatgc cgcactggtc aggatcagga agatcacaaa
       61 ctttcttaac ctcattaaac cgactccact agatatggta tcggctcttt ttcaaaattt
      121 attgaaagga acttgttgaa atattatagg gcttaaaaaa gagattcccc ctatgagaca
      181 gattagggaa catagtgaga aacttccgtt gtatatcttt acttttcgac aggagctgct
      241 tttaattggc cgcggaactt atctaaaaac tctttccaat ccgaattccg ctcttttctc
      301 gctaggattt ccccctttgc ggacaataag taaagactcg tggaatgggt aaagtccccg
      361 ccccccactt ctttatagct gaaccctaaa atagccgcca tttctcgtac ttgatcttta
      421 tccgaggtca acaacttcca acgcttatca aggtgcattt ttttatgata tttttgcaga
      481 acctcggcag tatcccggac agggtcaatg gaaaaagtga aatattgaat ttgctccctt
      541 tcttttttat ttaattggga atcaaaaaac ttcatatctg aaatcattaa aggacaagcc
      601 ccaggacaac ttgaaaaagt catggtaacc acggctggca agccactgag atcttttaaa
      661 actaaagatt tattttcctg agtcgtccaa ctttgctcaa gttgatagat ggattttgaa
      721 gggacttgat ccttacttat ggaaagtgcg aaatttggca atcctaaaaa taggaacaaa
      781 aataagaaat tcattgagac ttctcctttt ttaaatcgct tgcacaccta aatcctagat
      841 tccgcatgca atagtctcct ttgagtccgc tgcgaaaacc gtacctcata aatgaagcgt
      901 aggatttgga gtctaaagaa ctcacggatc cccctccaca aaaaaagccg tccgttcgat
      961 cccctttcga tcttgaatca gaagagatca tgactgagct aaaatcttca atccattccc
     1021 agattaaacc atgcatatca tgcacgccaa atttattggg ttttcttaaa cccactttct
     1081 caataggctg atcacctgtt ttggcatacc attctaataa ttcttttaaa atatcggggt
     1141 tctcggggtc agaggcatat tcccattcgg caattgtggg taatcttttt cccaaagtct
     1201 gacaatattt cttggccaca aaccaagaca cataagtgac gggattagcc cccattgttt
     1261 ttagctcatc ccctgagagg acttcggact tccaatcctg gagatagcgc tgatctacgt
     1321 ataaagaaat tattttattt ttcttaaatg aaggattttt caaaataaat tcattaaaat
     1381 ccgcatttgt gactggatac ctatccaaat aaaaaggatg gatctcgata tcctgatctt
     1441 ttaattcttt aaaaaaaggt ttgaattttc cagctggaat taaaatctga tctgcactca
     1501 atgcgctagt ccaagaccca ttaaaaaaca taacaagaat gaggctagcg attgttttca
     1561 tagaattatt ttcgaaccga tttaacttct gaaggcgtta cctcgtcaga tttattaccc
     1621 caagagttac gaacgtaaga aagcacattg gcaatatctt cgtcattcaa tcctaaggcc
     1681 ggcatcactc cattgtactc gactccattg atcacagcct ttccttgcat gccgtttact
     1741 atggtgctta ttgcacgctt tttatcggcc attaagtaat cagattttgc cagcggagga
     1801 aaggcaccac ttaagccctt gccgtcagct tggtgacaag cggcgcagtt ctgagtaaat
     1861 actatttttc ctgcggcaat cctctcctcc aaatttttgg caaagatttt ctcaggaact
     1921 ttcccagcag gcatcgtttg cgtagctcca ccttctggca agtagaccgt gtctgactgt
     1981 ttcccagtat aaatcatagg atctttatcg cccacaacct ttaacattcc taaagccccc
     2041 ttattaaaag ctctaaaaat ggcgtgatcc accaaaatat atgttgaagg aatatcaatt
     2101 ttaaagtcaa cgatgacgga gccaccggga ggcaccactg tggtttgcac gttttcttgg
     2161 accacctttg ttcctccttc cacataaacc ttgtcgaaaa tttctccgat cacatgaaaa
     2221 tttgaggcta tgttaggtcc tccagtgcca aagaaaattc tgactttttc ccccactttc
     2281 gcattcactg aattgtcccc ggctaaagca cctacacttc cgttaaatac aacgtaatca
     2341 ggctgttctt taattgcttt ctccatatca aatgcttgaa ggcccttttc accgtatttt
     2401 cctttagtgt aaaaatccga ttgaaccaca taaaattctt tatctacttt aggtaagcct
     2461 tgctctggtt ctacataaat caaaccatac atgtcgtttg caatatgcat ccccacgggg
     2521 gccgttgcac aatgataaac atagagtccc gcattcaaag ctctaaaaga aaacttagag
     2581 ctatgtcccg gaatggtcat agagccctca gcgcccccgc cctgtcctgt gaccgcatgc
     2641 aaatcaatat tatggggcat tttactagag ggatgattat gaagattaaa ctcaacataa
     2701 tcgccgacgc ggattcgtat cataggaccc ggaacggtac caccaaaggt ccagaagttg
     2761 tattccactc catcggcaag ccgacccgta acttcttttg tttccagatc gactttcaca
     2821 cgagcgggag tccttcgggt tattggagga gggaccttgg gggccgtcgt aagaatagct
     2881 gtttcttctg gaaaattttt agggtccgca tatttggaat tcacagcgga ctttgccaat
     2941 gaaacgcttg agagaattaa caaacaggtt gtaataaaat attttttcat aaagcccctc
     3001 ctttaagcct ttttagccca tgaattacaa tagccattag ctactacaaa tttattaggg
     3061 aaaatcgcgc aggaaccgac ctctttcccg ttcttctttt cgggtttaac aaaaagtatg
     3121 cagttagcac atttatttcc cgcagccttt ggagatttgg tggattcttc gacgtaacca
     3181 acagctttag ctgtggcctc gctcggatcg accagttgcg aacccgcatc gccagacttc
     3241 ttgcggcgcc cttcttcggc gaaagccatc ttacctgtaa atgcagaaat taaagctggg
     3301 gcagcaatgg ccacgccaga aattttagcc atatttttaa aaaactctcg tctcttcata
     3361 agtctgttca taggtctctc cataggaact ccgatcgtta tcgagtggat tgcatgcctc
     3421 attctcgtca aattcgagaa aatgagacat gattcaaatc aaattttctg cgttttaaca
     3481 tattgcaaga tattatatga tcatatatag atataatatc cactaagaaa ggttgacttt
     3541 aagtgtcaaa acttccccca gcccctactg aattttgtcc catttgttca agccatgaag
     3601 aaaaagtgac cgaccagatt ctgcaaattg tgcaaaagaa atcttatgca aaaaattgtg
     3661 ttatctttga acaagaacaa gaggccagtg gaatttatct tatccaaaaa gggagcgtaa
     3721 aaatttctcg tatttcaccc ctagggaaag agatcctcat tgaaattctc agtgccggaa
     3781 aaacctttgg agccagtggt ctctttggtc agggacgcca tgccgacacc gcaaccacca
     3841 gtgaaaattg cgagcttttt gtactcccaa aaaaagagtt taaagaaatt ctctccgccc
     3901 atcctgaact ttaccattcc gtgattcaat ccctgtgcga atggatggat cgactcaata
     3961 atattattga aaatatcaac accccctccg ccagggaacg ggtcgcttct tatcttaaaa
     4021 gaactcaaca ggaccagaac aatatcctca tccatctcaa tggaaaaaaa cacgaagtcg
     4081 ctatgatgct cggactcaga cctgagacct tttcaagagc cctggcagag ttggaagcag
     4141 aaggcattat taaaatgaat cacaaacaga ttcagatcct gcttcctgta aaactttgaa
     4201 ataacgtccg tatttgctcc taagattggc aaaatattga tcttaatcaa cgaaaacaac
     4261 ctctcctaaa tctatcatta gctgtggtgg agggagattt ccatgcttaa gctcaaaagt
     4321 gacactatta aaattcatga accttttgcc gaatatctcg gaaatacgcc ggaaatcatc
     4381 gattttgaaa tcagcctttt ggatgtcgca aaactcagcg gtcatctctg tccgagtgtc
     4441 gctggggcct ttcttttaac tcgaaaagcc gttgaactgc tttatccaga caccaaagtc
     4501 tgcattcgtg gacagatgga agttcatctt ccgggagctc ctgatgaagg ccccctgggg
     4561 ccgatggcta atgtcattag ctatatcacc ggcgcttggt ccgaaacagg tttcggagga
     4621 ctcggtggaa ctcggtacct tcgaaaagat cttcttcatt tcgcttctcc tctttgtaaa
     4681 aagcgggaat ttgtcttcat tcgcttagac aacaaaaaaa ccgcttcggt ttttttcaat
     4741 ccaaaaccga ttctacaaaa acttacaaat agcgaaaaga cttttcaaga gtcttggcag
     4801 gcgaatgttg aagctattct caactcaagc gatgcgatag agagcttcta acttacagag
     4861 ctggattatc gctgcaaata aattttcaaa agatgatcaa ccagatcctc tggctgtttt
     4921 acgatctgat agttggagta cagaaacatt tgcgccagag tgcttttagc ctgctcttcc
     4981 actgtcaggg cgtgaacttg aatccgccgt tgggtcgcct ggcgaagcgc gtgcttgacg
     5041 tcctcaatgc cataatgacc ttcgtaacgg tcaaagtccg tgggttttcc atcactcaga
     5101 agaattaaaa actttttacg agcttttacc ttttccagtt gggctgaagc atggcggatg
     5161 gcggggccaa tacgagtgta ggctcgtggt tttaaactcc ataatttttt caagccctcg
     5221 atccagcttt cgtcccaagc tttgaccact tccaggcgac aatcctggcg ggtgtgacta
     5281 taaaacgcgc caatttgaat ttgttcagga taaaggccca tggcctcgcc aaggagataa
     5341 atggcttctc tttcgaaatt caaaacacgc ttattattaa cccaggagtc cgttgataaa
     5401 ctggtatcaa tcaaaatata ggaagcccaa tcgcaatctc tccgctctcg gcgcatatac
     5461 aagttctcgg gcggtgtctg tcccgcctgg agaaaactgt aacggtctac catctttgac
     5521 agatcaatct ccggtccgtc cttctgacct cctcgccagc ggctcattga aaactgtttt
     5581 tcaagttgct gcctcagacg ttcaatatct tttttatact ttttccgcag gccgagtctg
     5641 gcctcctgaa aatcttccct taggaattct cctttaatta cagactcgcg cagggtacac
     5701 cagtccggct ttaaaagctt cttctgataa tcccactcag ggtagagata tgtttgcagg
     5761 ccgtcctgcg ggctggagat agcttcatct ttaatctctg gaacattgat attcaaatga
     5821 atatccgccc gatacaaaga cgccgcacct tcttccgtgc ggatgacgga cttcatctct
     5881 agctcgtcaa tagcctcttc atgctctaag atttcatcac tgccatccat atttttaaag
     5941 ccgccattat agtcctcggc cgtttttact ttttcaaaaa tatgaatcaa aggactgtcc
     6001 tcttgttttt tcagagggat ctttttcact tccgcttttt cacggatctt cccttgaagt
     6061 tcggtgccct ggggaaatgc actttcctgg gccctcaagg agtccggcaa atccaacacg
     6121 gcctcaccct cggcctgcct tcgataaaca agaccccaaa gcagtggcca attttgatag
     6181 tgggttttat taagattctc tatagttaaa ctgttttccc aaagcgcttg ccaagggtct
     6241 ggactcaagg tttgaattaa atcttcttct aactgaacaa actggggcag ctctgcttga
     6301 agacgcaagc tcactcggcg gagatttaaa aaataagttt tcaaggccaa ctgttcattc
     6361 acctcaggat tggctggaaa atatatattt tccaggtcaa aaccaacgga tatgagggtt
     6421 ctgtaaacat aggcctgaat attttttttc tcgtctgggc tgaggctcat ttttgccggc
     6481 agataaaggt gcaagccttg aatccccccc catttttcag cattatggat ttcataagcc
     6541 ctgcccccat aggcgcgggc taaggtttgc aaaactcctt tgagtccctc aagatgaacc
     6601 accccaggcg catcaatcgc tggatttttc cagtccctgt atttccggtg aatccaccca
     6661 aaaagctgtt catcaaagct cataattaaa aaaccagtgc cgctaaatta ttgagaccct
     6721 ctgctatttc cccatcatcc gttaaggcct ggacgatggc aacatcgcaa gcttggcgtg
     6781 gtgaaagacc tgattggatc aactttcccg caccaattaa aagccgcgtg gatacggtct
     6841 cggtcaaacc cagttcctga agatttcgca ctttctgtcc gaaaaagact agctttccag
     6901 cgacactttt ctcacaagta ctttcagctt gaacaatctc agtctctaat tccttatggg
     6961 ggtaattaaa agacaatcct agaaatcttt gccgagtcga tggcttcatc tctttgaaag
     7021 ccttttgata gcctggattg taactcacca ccaacataaa ttcttttgta gcttgcaaaa
     7081 gctcattgcg tctttccaaa taaagatgac ggcgatgatc cgtcaaggga tgcagaacca
     7141 ccaccacatc ttcccgagcc tcggcgattt catcgagata aaggatagcc ccttcgcgca
     7201 cggctcgagt cacggggcca tcttgccaaa ccgtatcgcc cccctgaaca aggtagcggc
     7261 ccatcagatc aatcgctgaa gtctcatcat ggcaggcgac tgaaatcaga ggacgaccca
     7321 tctcttccgc cattttttca acgaagcggg acttgccaca acctgtgggt cccttgatca
     7381 gcaagggcaa ctggttgctg taagcatgct taaaaacttc aacttcatta cgaattgctt
     7441 tataaaatat cacgtcccac ctctccgtga gggccaccgt attttacgaa attatagata
     7501 aaggcgataa tgccactcgt gaataagcct gccgccaaaa taagtccaaa gaagtgcact
     7561 tcaagctctt tctgggtggt caaaaaatcc atacccattc gacgttctaa ataaacttga
     7621 gtgactcccg caatcccaaa agccacggtc attgcaatca tacccacatt ggaagtccaa
     7681 aaagcaaaga ctgaggcttt ggaatcatgc aacttacgcc cggttaagga gggcatcgcg
     7741 taggtcataa ttgccaatat caacatcgca taggctcccc aaaaagccat atgaccgtgc
     7801 atggctgtaa ccaaagttcc gtgagtgtac atgtttacag aaggaagggt gtgcgcaaaa
     7861 ccgagaaatc cagcgcctac acaggacatc actgaacagc caatggtcca agacaaagcc
     7921 gtcttgttgg gatgttgccg tccccctttt ttcgccagag tgattgcata gatggccatg
     7981 cccaagaaag ccaaaggctc taaggcacta aaaatcccgc caatcatcaa ccaatactta
     8041 ggagttccaa tatagtaata atgatgacca gtgccgagaa ttcccgagag aaaagtgaag
     8101 cccacgatga tatagagcca cttttcgatg atttcgcggt cgataccggt aagtttaatt
     8161 aataggtaac tcaaaatagc acccataatc aattcccaaa cgccttccac ccaaagatgc
     8221 accacccacc aacgccaata agaatccatg gtctgattcg tggtactgat cattccggga
     8281 agataaagca aagccgcact gagcagaccg aaaaacaata ccatgctcgt cgtggttttt
     8341 cttttcccct tccagatcgt cattccgatc aaatacagaa acagcaaaac atccacgacc
     8401 acaaagtaat ccaggggacg gggaatttct aaaaacttgc gaccttccca ccagttaaga
     8461 tgaaaaccca ccactgcggt cacgccgacc accacaaaag ccactagctg gagaatcgcc
     8521 aactttggat ggaccaattc actgtcggct tcttcgggaa caatgtaata agccgcgccc
     8581 ataaatcccg tcaacaacca catcagaaga agattggtat gcgttgccct ggccacatga
     8641 aaagggatcc aacggtgaag accctcgatt cccatatgat caaaagccat aataaagcca
     8701 tagaccagct gcaaacccag gagcaacatg caaactgaga aaaagtaata tgctattttt
     8761 tgcgtttgat atttcatatc gtcatcctac ttgttttgcg ataaaaaatc ggccaaagct
     8821 tggatgtcca aatcctgcaa tggcaatttc ggcattttag aatctgcttt aatttgagtc
     8881 gggtccttta accaggccat cagaaaggcc ttatcacggc gcgagcccac tccatcgagt
     8941 gcaggtccta cctgcccgcc tcgacctccc agactgtggc aagcaatgca catttgatca
     9001 aaaatcaccg ggtgcggaag agtcatcgcc actgccaagg gtggctttgc aggaaatccg
     9061 ttgctatcaa tttcaccaac ccacttcaag aaagaaacta agtcttcacg ctcttcctcg
     9121 gtaaagttgt acttctgcat ctttctttgt ccgggataca tagattctgg atcctgcaac
     9181 attttcttaa taaaatcggc tcctctccgc tgaaagacct tggtcagttc gggcgcgtag
     9241 taggcgcctt caccgagaag cgtatgacat cccatgcaat tacttttgtc ccaaagatcc
     9301 tttccgcggt gaacgctgac cgtaatattt tcctcgtttg tcctggctgg aacttgcttg
     9361 atggtgtcaa gggttaagag aacaaaggct cctccacaca ccgctgtacc aatcaaaaaa
     9421 aatgattttg ctgctgattt tgaaagcata aatttgatcc tttactatat tagatagatt
     9481 agtgaaacga aggggaaaga accttgatct tgatcaagtt taagtgaatt aatttttatt
     9541 ttaaaggccg ttgcctcact caaaaaactt ggattattca ccgaacaaaa actaactgag
     9601 ttatggccag gacggccata actcagcgtt ctcttggatg aaagtaacat cacgtcatct
     9661 gactttaaat aaccgactct aaaatagaag ccccctgacg agtcgggata attttaggtc
     9721 cctgcggatc atgataaaat ctgcgaaccg cttctttttg ccgacctgaa ctaaaagaag
     9781 aaattctttt caaataacca atgactcttg tcgcatgatc cacgtcctcg ctcttgcatc
     9841 gagaacactg ataaagagtt cttttatcga tatgatcaca atcgttgcaa atcgtgagtt
     9901 tgacattcac acaccagtaa ttacatccgg cccgagccgc cgcgcaaaga agcttataat
     9961 aagcctcctt ggtcagtttt tcctccagat taagatgcaa agcggagccg ccatccaagt
    10021 attgcatcat ttcctgaccg tgcatttgaa ttttatcaat tggagtcacg ctgtcgtcct
    10081 caaccacata aaagtaggag ttgtaacaat cccgcgggac catcagaccc tctttacgat
    10141 cccattgcgc attttttata cctaaatttt cagcgggcac aaattcagtg ttaaacatat
    10201 aaccaaattt attttttgct cgtttgttgg tctcaaagat gatttttaat ttttcccgca
    10261 caaatttttt gtaacttgct tcattccgag ccaaaagtcc ttgcgattcc gccgcctcca
    10321 ccattccatt gattccaatg gttaaaaact gtttatcaag gcttataaaa cccgctgaat
    10381 aggccgttaa caagcccgcc ttaaagtatt cctccatcaa acgccggtaa gccacttgat
    10441 acttctgcac tttttcaaca cactcttgaa gatccaactt cttttgtgta atacgattca
    10501 tattcatggt gatgacgtta atagatcccg tcgcgacacc ccctgctccc aatgaataac
    10561 taaaagtctt atcggaaatt tcgttacgaa gacggcaaca acttgccaag ctgtcggcag
    10621 actcgctcat atacataaaa aaactatttc cttcgctgag ctcctgagcg cacatcgaag
    10681 caaaagcatg gtctcttggc ttttgcgaat ccaccagcat ggcggcggtg accaccggaa
    10741 aagtgagcag agcctttttt ctttcactat tgaaccaagt cataaaatga ttttgcagct
    10801 tggaaacccg tccccaatca ggaacaccgc catcaggaaa aacaaaatcc ccgaacaagg
    10861 atttaaaata ataatgatca taaaccgaaa tgttccaaaa aacggattga tacccccggg
    10921 ccgccgccgg ttggtttacg gcgtaaacga cgtgctggag atgattatct ataatcttgg
    10981 tatgagtatt taaataatca gctccgtagt ccttactagc aaagtgatca aagtacatga
    11041 gccactcaac tgtcgcgaca gccccggcaa attgggagga aatagcaaat accaaattca
    11101 caaaggtccc acaaaagctc tccaaatgct taggcgcttc ggactccccg cccagttttt
    11161 ttaagccgtc gatcaatagc ggatacatgg aaatagaagc acaataaggt ttcaaagagg
    11221 tctcgtcatg cacataaatt tcatgttctt gaatttgtct cacatattca caggccagtt
    11281 ctttatcaaa aatttcagag atcttattgg ccaccaattg gcgattcact tggatattaa
    11341 tgtctttatt gatttccgcc tctagcgtgg cgatattttt atgagcgacg ttggcattgg
    11401 catccagctt cgagccatcc gccgcatttt tagcgtggag atagctttct ataaaattta
    11461 ttttttgctg aatttgctca tcagtgagac gggtcaacat agaaactcct ttttttatta
    11521 tgatgggggt tgaaaaagat gattaagaat ttgtttttta ttgagatcta aaaaaatctg
    11581 attggtcatt ggcgaagcaa gcccccccaa tgccggattc caagggccaa gctttgcaaa
    11641 atctaagttt tgctctaaga cttttggaat cttttccaga ccagtataga gagccgtctt
    11701 atattttaat ttccgagcga ttttaagaaa agcattgagc tgggcaagat gccattctcc
    11761 ccccatgaaa agtacacagc tgacatagcc ctgatagcgt tccagcaagt gcctatagaa
    11821 ctcagaactc aaatcctttc catttttttg ggtccagagt tccggcgagt gacatccctg
    11881 acactgaaga ggacacccgc atacgtaaaa cgccaagctg atttcgtcgg gaacttcctg
    11941 aaagactata tcgtacttgt agatattcat aaccacgtcc tctacccctg aggttaacaa
    12001 ccactttttt taaatcacta gggattgtgg tttttgttga cctgagacac tagatacacc
    12061 gcaaacaggc tcaaaaattg atctagatca attttgagtc ttatcaactt gtggggattt
    12121 tccaagcatg acagcctagg aacgtggagg cagcatgttc gataaaatta aaacaataca
    12181 atctctcgag ctctttcaag atgttaaaca ggaggatctt gtggtcatcg agaaactggc
    12241 tgaatgcctc catattaaaa acaatgaaac cttgttccac gagaaagatc ctcaaaaaaa
    12301 tgtctatgtt atcgtctatg gctcttttaa aatactcagc aaagaccagc aggacaatgt
    12361 ggtcattttt aactttcttg gaagaaatga ggtgctggga atatccatgg cccgcttagc
    12421 caatccccga tttccagttt cagccgtcgc caacgaagaa agctgtgttt tgaaaatcag
    12481 cgtggccgat tttaataaat tgtttttaaa aaaccctttg cttgagtcga ggattcagct
    12541 tcagctcgcg caaagatttt tggattttca agaagaccgc tgtttgagaa aatccctggc
    12601 ctcacaaaga ctcgccgagt tcattttgcg caccctcgat cgacaacctc aatcatgccg
    12661 tca
//
LOCUS       DATBTE010000173        13652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_432156, whole genome shotgun sequence.
ACCESSION   DATBTE010000173 DATBTE010000000
VERSION     DATBTE010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 13652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13652
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432156"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..848)
                     /gene="lipB"
                     /locus_tag="VIG33_06465"
     CDS             complement(144..848)
                     /gene="lipB"
                     /locus_tag="VIG33_06465"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY8270515.1"
                     /translation="MKSRNKFMEQEEFIEQSVPYKIQNWGLMEYSQALELQEKLVAEI
                     AEKDQPGTIVFCSHPPVVTLGRGTKEGDVFAWQGPIMEISRGGRATYHGPSQLVVYPI
                     INLNRASPARPARDVGAFLRNFETAIVRTLQEYGIPSEGRSLKKKSELETATEETGVW
                     VGNQKIASLGIGVRKWVSFHGAAVNLDQDPLAFQGLRPCGFTKETMVSLQQLIGDPVD
                     RKQFMQRLFSHLGEWL"
     gene            complement(832..1758)
                     /gene="lipA"
                     /locus_tag="VIG33_06470"
     CDS             complement(832..1758)
                     /gene="lipA"
                     /locus_tag="VIG33_06470"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY8270516.1"
                     /translation="MPLDTSTKLKTPSKGAPVIAKPNWLKVKAPTGENYTRIKEMLGD
                     LKLATVCQEAKCPNMGECWAGGTATFMLMGEVCTRGCKFCNIKTGNPRGVLDPLEPEK
                     VGYSIAQMGLEYVVITSVDRDDLPDQGAEHFARTVRTIKELDSNVIVEILTPDFRGMD
                     ALITQLAESKPDVFAHNIETVDRLQKYVRDPRATYKQSMHVLELVKKFDPTRFTKTSL
                     MLGLGETDEEILQTLKDLRHIHCDVVTFGQYLQPTQNHLKVEEYVTPEKFQFWQKTAE
                     DLGFLYVASGPLVRSSYRAGEFFMKAMIEKSK"
     gene            1912..4404
                     /locus_tag="VIG33_06475"
     CDS             1912..4404
                     /locus_tag="VIG33_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8270517.1"
                     /translation="MESSNPQGRENLERLTGIVERITYHNPENGWTVMKVTPFRDPTK
                     LVAVLIHQVKVFAGATMEFQGSYSHHPKHGEQFKAIRAIEKKPATAAALEKYLGSGLI
                     KGIGPAIARRVVGHFKDRTLEVFESKIEELLDVPGIADKKLLQIRLSWEEHKAIRDVM
                     IFLQGYGISTLFATKIYKAYGNKAIQIVSENPYRLAHDIYGIGFFSADRIALAMGFEK
                     NGTPRIEAGIKHVLSASREQGHCFLTEKQIIIQTPELLREPLEEDKITSVLTVLLSSD
                     QAKSRKLDRGEDLETCYYSKSLYFDELTTAKQISELSKIGIPFNAERVHAWVTKYCEK
                     HLIELSDEQFQAVCGVVGQSFSILTGGPGCGKTTTTKVLVQLLFAMKKRVLLAAPTGR
                     AAQRMSEVIGSEAKTIHRLLEWAPDKGGFKKDDKDPLVCDFLIVDETSMLDISLSASL
                     LKAVPRGAQVLFIGDPDQLPAVGAGDVLSDLLRTKSISRYRLTKVFRQAQASTIIRYA
                     HEINTGSVPKILSPLAQPKAFQEGNDCLFIDADEATKDQIQFIQRAKQAMDSAKANQH
                     GKIEVDKLYRPDLFDEAAVRAPVLTIPDKFKHVDLQKLAAAQSGIEELFQVIKNVHPW
                     SSLHFGMTALDTVVRLYTKSIPEWLGKDVEIQVLTPQVRGSVGTVNLNTCLQSVSNPE
                     ASGKRQLLIGEKILRVGDRVIQTRNNYDLGVFNGDIGRIAEVDPEEMNCEVVFSGGEE
                     RRVVFEKEDLSELSLAYAITIHKSQGSEFQAVIIPVLGQHFNMLFRNLIYTGLTRAKK
                     LAIFVGTRRAFAMAVGQIDNRKRQTALTDLIK"
     gene            4518..6182
                     /locus_tag="VIG33_06480"
     CDS             4518..6182
                     /locus_tag="VIG33_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270518.1"
                     /translation="MRFRFLLVKQSVSQVETALVVLLLQIFLSTTAFANSQWPAEVIQ
                     QLEKWQNITSISHDESGPEITNVFTKRWEFSDANGIKSLTEQLPLSQHVELNNPADFS
                     QGGTIHWVSSTINLSENISVQPFVLGDSTALNSLSALQIDQKRIVLTFNDNQGKVKFK
                     VSLLQTSPATPGTNRFSAENRHLLGIIQTPDFGQTIGNVMRQRMFGISIQDFPGPRYY
                     LNHPIENGRRRIIISPEFPDSYLPVLNKVIKKWNTLFGRNYYSFFKKEKLNMVDCLSS
                     DLLCIQWTGGARFTWTQYSGTTDLAADPGSGHVLGGVIYIHNNADLKKAREGTPEELS
                     KLMKPSNLFDVAISFSKFAIYSTIWREKSDDLVEGMLLHEMGHFLGWDHNYAGAWRGT
                     LNSPGDTVMGYPPFLLAGRLNNLGSLDMEKFRALYFLNSQSITSPYCGDLDIGPILNK
                     ETGLASKRIPECFMWTTGNTADWLTFQATIFGNLGTTFGYGVFQLMNPPSWFGSYAVS
                     LGYIAQNSNSAENRRKATEHLCRQRARDTALLGILHSTIQFAPKCQ"
     gene            6304..7035
                     /locus_tag="VIG33_06485"
     CDS             6304..7035
                     /locus_tag="VIG33_06485"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HEY8270519.1"
                     /translation="MNLKEYQLKRANLLLQAPKYRVLCSACAQPDFGCYCAQVQSFDA
                     KINFVVLIHPVEVKRRIATGRMSHLCLKDSYLIKGQNYTEDNLVNELIQDSEHHSVIL
                     YPGPASKNLSLLPTKEKANLFPKNKKLRIFVIDGTWATARKMIRQSENLNVLPRICFS
                     PEKPSNFRVRKQPHPNCYSTIEAIHHTIELIGESQGFSVKKREHDKLLQVFDYMVERQ
                     LGFIRESNLNLRVASYRREGQKKIS"
     gene            7074..8231
                     /locus_tag="VIG33_06490"
     CDS             7074..8231
                     /locus_tag="VIG33_06490"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HEY8270520.1"
                     /translation="MKESRNSFDWERTVILELNREHEYTCRIHRINLRPISILLFDSA
                     SLWGQFDNRTRTISISRKLVNEYSWHHVVGVLRHEMAHQYIAEKSPLEFISENPHSAL
                     FKEACKRLGVPDQFAKAGINLQSCNLDWKTEPKDEATEKILDKAKRLLALGTSTNEHE
                     AHLAMERVRELYAKHNLEHMENLSQENFVHLVITHGKKRAESWELRTISILTEHFFIK
                     ALTLQQFDANSGKRFHAVELIGTRENVTMAEYVYHFLLQQVEFLLRQASNREMGLVRA
                     ERTSFRLGVLDGFDKKLKLSTQNIPKDSPDENNKEQTVIGRALTKFRADPYLEDYLSD
                     IYPRLGHRRRSSLEIDGNAFAAGHAAGKSINLNKPITTNMGNQGRHLGGGK"
     gene            8584..9195
                     /locus_tag="VIG33_06495"
     CDS             8584..9195
                     /locus_tag="VIG33_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270521.1"
                     /translation="MLRIVQIFGLVFFISHFTIASVEDPQFLLPKVDAFLAKPSFASA
                     FKAGDKIKYVTSDCIGERPCGSGVSMTAVIDPISSDEICINQFGPDNSLKAKDKVNLQ
                     QWDNINRNYLRAKINKIESFGFKVSVTRFENITCPQEVYLGPESQCAKVSLQGVNGLN
                     NTSEYQYILWNSPIGIAQIISSTQTDHMLFTRTISYRVTEVYR"
     gene            complement(9276..9611)
                     /locus_tag="VIG33_06500"
     CDS             complement(9276..9611)
                     /locus_tag="VIG33_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270522.1"
                     /translation="MFKFLVAFITSLTLPTVIFARTVDFECLTGKPETVAYDSAIVFY
                     AKMNFSKQTPVLIYRHREFPGYSSDEGYQFFINLKNISTLKIQYIDRLGTGMSYSKAS
                     LNDQLVYCL"
     gene            9981..10226
                     /locus_tag="VIG33_06505"
     CDS             9981..10226
                     /locus_tag="VIG33_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270523.1"
                     /translation="MEVEIKPTGIAVEISCLRCGTKALICKSCWRNQRYCSTVCSREA
                     KLERHRRDQKLYRMSPEGRERNKVHQRAYRQQKKIQE"
     gene            10373..11248
                     /gene="istA"
                     /locus_tag="VIG33_06510"
                     /pseudo
     CDS             10373..11248
                     /gene="istA"
                     /locus_tag="VIG33_06510"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            11982..12671
                     /locus_tag="VIG33_06515"
     CDS             11982..12671
                     /locus_tag="VIG33_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8270524.1"
                     /translation="MKDQEREIISKYLSQWIQREKSERHTSMIQSKIRTAKFRRVETV
                     ENYNFRHSKMTEKIEKTYLALHNGINKDNLPSAVFTGHAGTGKTHLSRALGYAACQKG
                     LSVLFITAAEMVNHLQHAQKTYNLEAELNKYRKPQVLIIDELGYVALDTQASNLFFQV
                     ISTRHDAELGTIATTNLPFGKFNQIFANDAIAHAIVDRLVNEAEVFYMEGDSYRPHQR
                     QEKLKRKKQNA"
     gene            12698..12922
                     /locus_tag="VIG33_06520"
     CDS             12698..12922
                     /locus_tag="VIG33_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270525.1"
                     /translation="MAQLNLDGQGHLISDRKKSLYRMRASDPGGLRKCAKRSLSRLQR
                     ALENAKEEKQAHGEIVTYHPGFLFCQDTYL"
     gene            complement(13050..>13652)
                     /locus_tag="VIG33_06525"
     CDS             complement(13050..>13652)
                     /locus_tag="VIG33_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270526.1"
                     /translation="VYMTGVGTLKTDGNETVDCAGLSYTGAMTIFNVRKFQIENSVLM
                     NNQTEDALNVYKSNVEAKSIRIEGSASDAFDCDYCVFKGNNLLLRNNLGDGLDISGTL
                     AFVENSNMDHQSDKNISVGENSRAFVVHSVLNESHYGVAAKDSSFLSLNDVTIQHNEI
                     GIGTYVKKAYYLPPQVEVGGKNKIADNGENEKFEGYKPVK"
BASE COUNT         3962 a         3001 c         3035 g         3654 t
ORIGIN      
        1 ttttcttcaa gtgctttagt gtcctgaccg ggaaccaagg aaatctttat gggattccct
       61 gaagttccaa cttctgattg cgaggtgcaa tttacaaata taaataggct aagaaacaag
      121 ataataaaat ttttcataag catttagagc cactctccca agtgagaaaa caatctctgc
      181 ataaattgtt ttctatctac aggatcccct attaactgct gcagacttac catggtctct
      241 tttgtaaacc cacaaggcct taacccttga aaagccagcg ggtcttgatc gagattcacc
      301 gccgccccat gaaaactcac ccatttgcgg acgccaattc ctaaagaggc gatcttttgg
      361 tttccaaccc aaacaccggt ctcttcagtg gcggtttcta attcagattt tttcttaaga
      421 ctacgtcctt cagaaggaat gccgtactcc tgtaaagttc ttacgatggc ggtttcgaag
      481 tttctgagaa acgcgcccac atccctagca gggcgggctg gcgaagctcg atttaaattt
      541 ataatgggat aaacaaccaa ttgagaaggt ccatgatatg tagcccggcc ccctcgtgaa
      601 atttccatga tgggaccttg ccaagcaaag acatcaccct ctttggtgcc tctgcctaaa
      661 gtgacaaccg gaggatggga acaaaatacg atggtcccgg gctgatcttt ttctgcgatt
      721 tcagcgacta atttctcctg aagctcaaga gcttgggaat actccatcaa accccagttt
      781 tggattttat agggcacaga ctgttcaatg aactcttctt gttccataaa tttatttcga
      841 cttttcaatc atggccttca tgaaaaactc accggcacgg tatgagcttc gcactaaggg
      901 ccccgatgcc acataaagaa atcccaagtc ttcagctgtc ttctgccaga actggaattt
      961 ttccggagtt acatactcct ccaccttcaa atgattttgg gtcggctgta aatattgacc
     1021 gaaggtcaca acatcgcaat ggatgtggcg aagatctttc agagtttgaa gaatttcctc
     1081 atcggtctca ccaagaccca gcattaagga ggtttttgta aatcttgtgg gatcgaattt
     1141 cttcacaagt tcaagcacgt gcatggattg tttataggtc gctcgtggat cacgaacata
     1201 tttttgcaag cgatcgacgg tctcaatatt atgagcaaag acatcaggtt tagactcagc
     1261 cagctgagta attaacgcat ccataccgcg aaaatcagga gtgagaattt ccacaatcac
     1321 attcgagtcc agctctttaa ttgtacgcac ggtacgagca aagtgctccg cgccctgatc
     1381 aggcaaatca tctcgatcga ctgaagtaat gactacgtac tctagcccca tctgcgcaat
     1441 ggagtagccg actttttctg gctctaaggg atccaagacg cctctggggt ttcccgtttt
     1501 gatattacag aatttacagc ccctggtgca aacctctccc atgagcatga aggtcgctgt
     1561 gccaccggcc caacactcgc ccatattagg gcattttgct tcctgacaga ccgtcgccaa
     1621 cttcagatcc cccagcattt ctttgatccg agtgtaattt tcaccggtgg gagcttttac
     1681 tttcaaccaa tttggtttag cgataacggg agcgccctta gatggagttt tcagttttgt
     1741 tgaggtgtct aacggcatgg caagtgtcct tttttaaaaa tcaaataaaa ccgttttaga
     1801 gggcaaaaaa gccagaggca agcaatatta tcatggcctc cctattgcaa aaagaacctt
     1861 ttcccaataa aagttcccta atatcaccac caaaagctca aaggatctta agtggaaagt
     1921 tctaatcccc aaggacgaga aaatttagag cgcctcactg gcatcgtcga aaggatcact
     1981 taccataatc ctgaaaatgg ttggacggtg atgaaagtca ctccttttcg ggatcccacg
     2041 aagctcgttg ccgtcctgat ccaccaagtg aaagtattcg ccggtgccac catggagttc
     2101 caggggtctt actcccatca tcctaaacat ggcgagcagt ttaaagccat tcgcgcgatt
     2161 gaaaaaaagc cagcgaccgc cgccgctctg gaaaaatact taggttcagg cctgatcaag
     2221 gggattggtc ccgctatcgc acgaagagtc gtaggtcact tcaaggatcg cacccttgag
     2281 gtctttgaaa gtaaaatcga agaactcctc gatgtgccag gaatcgccga taaaaagctg
     2341 ttgcagattc gcttatcttg ggaggaacac aaagcgattc gtgatgtcat gatttttctt
     2401 cagggatatg gaatcagtac tttgtttgcc acgaaaatct ataaggctta tggaaataaa
     2461 gccattcaaa ttgtctcgga aaacccctat cgcttagctc atgatattta tggaattggc
     2521 tttttttccg cagatcgaat tgcgctagcc atgggatttg aaaaaaacgg aactccgcga
     2581 attgaagccg gtataaaaca tgtgctctcg gccagccgtg aacagggcca ctgttttctc
     2641 actgaaaaac aaattatcat tcagaccccc gagcttttac gagaacccct tgaggaagat
     2701 aaaatcactt ctgtcctcac cgtcctcttg tcttcggatc aggcaaaatc ccgaaaattg
     2761 gatcgtggcg aagacctcga gacttgttat tattcgaaga gtctttattt tgatgagctc
     2821 accacggcaa aacaaatctc ggaactttca aaaataggaa ttcctttcaa tgctgaaaga
     2881 gttcatgcct gggtcacaaa gtactgcgaa aaacacctga ttgaattaag cgatgagcaa
     2941 tttcaagcgg tctgtggcgt cgtgggtcaa tcgttctcca tattaacggg cggacctggt
     3001 tgcggcaaga cgacgacgac aaaagttctt gtgcaacttt tatttgccat gaaaaagagg
     3061 gtgctcctcg cagcccccac aggacgagcc gcgcaaagaa tgagtgaagt gataggttca
     3121 gaggccaaaa ccattcatcg gcttctcgaa tgggcccccg acaaaggcgg cttcaaaaaa
     3181 gatgataaag atccgctcgt ttgtgatttt ttaattgtgg atgaaacctc catgctggat
     3241 atcagtctct cagcttctct cttaaaggca gtgccacgtg gagcccaagt tcttttcatt
     3301 ggagacccgg accaactccc tgcggtggga gctggagacg tcctctcgga tctcctgagg
     3361 acaaaatcta tttcgagata ccgactgacc aaagtcttta gacaggccca ggcttcaacg
     3421 attatcaggt atgcccacga aatcaataca ggctctgttc caaaaattct ttctcctctg
     3481 gctcagccaa aggcttttca agaaggcaac gattgccttt ttatcgatgc ggatgaagca
     3541 acgaaggacc agattcaatt tatccaaaga gcaaaacagg caatggattc ggctaaagcg
     3601 aaccaacatg gaaagattga agtggataag ctttatcggc ccgatctctt tgatgaagcc
     3661 gccgtaagag cccctgttct tactattccc gataaattca aacacgtcga tctgcaaaag
     3721 ctcgcggcgg ctcaatcagg aattgaagag ctttttcaag ttatcaaaaa tgttcacccg
     3781 tggtcttctc ttcatttcgg aatgaccgcg cttgatactg tggttcgtct ttacaccaag
     3841 tcaattcctg agtggctggg taaagatgtg gaaatccaag ttttaactcc gcaagttcga
     3901 ggatccgtag gcactgtaaa tttaaacaca tgtcttcaga gcgttagtaa tcccgaagct
     3961 tctggtaagc ggcaactttt gattggtgaa aaaattctgc gagtcggcga tcgcgtcatc
     4021 caaactcgca acaactatga ccttggcgtt ttcaacggag atatcggccg catcgcggaa
     4081 gtagatcctg aagaaatgaa ctgtgaagtc gttttttccg gaggggaaga gcgtcgagtt
     4141 gtttttgaaa aggaggattt atcagaactc tctttagctt acgctattac aattcacaaa
     4201 agtcagggaa gcgaatttca ggcggtgatt atcccagtcc ttggacaaca ctttaatatg
     4261 ctgtttcgaa atctaattta cacgggcctc acaagagcaa agaagctggc gatttttgtg
     4321 ggaaccagac gtgcgtttgc gatggcggta ggtcagattg acaatcgcaa acgccaaaca
     4381 gcgctgacgg atttgattaa ataatcaaac tttgtcactc gggtgtcaaa accattccac
     4441 cgaagaatgg aataaagccc tcatagagct tcaaagtcgt aataaacttg ctggcatgga
     4501 ttgcatataa aaggggtatg agatttcgat ttctcctggt caaacaatca gtgtctcaag
     4561 ttgagacagc tctagtggtg ctactgctgc aaattttcct aagcacgacg gcctttgcca
     4621 attctcaatg gcccgccgaa gttatccagc aacttgaaaa atggcagaac attacttcaa
     4681 tctcgcatga tgaatctggc cctgagatta ccaatgtctt cacaaaacgc tgggaattca
     4741 gcgacgcgaa cggtattaag tcgttaacag agcagcttcc tttgagccaa catgtagagc
     4801 taaataatcc tgcagacttc tcccagggag gtacaatcca ttgggtttcc agcactataa
     4861 atttgtctga aaacatctct gttcaacctt tcgtccttgg cgacagtact gccttaaata
     4921 gtttatcagc gttgcagatt gatcaaaaaa gaattgttct gacgtttaat gataaccaag
     4981 gaaaggtgaa atttaaggtc tctttgctgc aaacctcacc ggcgacgccg ggcacgaatc
     5041 gattttctgc ggaaaatcgt catcttctag gaatcattca aacgcccgac tttggacaga
     5101 ccatcggcaa cgtgatgaga cagcgaatgt ttggaatttc catacaagat tttccaggac
     5161 ctcgatatta tttgaatcat cctattgaaa atggcaggcg gcgaattatc atcagtcctg
     5221 aatttcccga ttcctacctt cccgttctga ataaagttat caaaaagtgg aatactctct
     5281 ttggtcgtaa ttactattca tttttcaaaa aagagaaact taatatggtc gactgtttat
     5341 cttctgattt actttgtatc cagtggacgg ggggtgctcg gtttacatgg acccagtact
     5401 ctggaacgac cgatctggcc gccgatccag gaagtggcca tgttctagga ggagtcatct
     5461 atatccacaa taatgcagac ctaaaaaaag ctcgcgaggg aactccagag gaattatcta
     5521 agctcatgaa accctccaat ctatttgatg tagcgatttc attttccaag tttgctatct
     5581 attctaccat ttggcgggaa aagtccgatg atttggtaga ggggatgtta ttacatgaga
     5641 tgggacattt tctgggctgg gatcacaatt acgcaggggc ctggaggggt acgctgaatt
     5701 ctccgggaga cacagtaatg ggctaccctc cttttctttt agccggaaga cttaataatt
     5761 taggttcctt ggatatggaa aaattcagag cactttattt cttaaactct caatccataa
     5821 caagtcccta ttgcggagac ctggatatcg gacctattct gaataaagaa acaggcctgg
     5881 ccagtaaaag aattccagaa tgctttatgt ggacaacggg gaatacagcg gattggctga
     5941 ctttccaagc caccatattt ggcaatttag gaacaacttt tggttacggg gtttttcagt
     6001 taatgaatcc cccatcctgg ttcggcagtt acgcagtttc ccttggctac atagcacaaa
     6061 acagcaattc cgcggagaac cggagaaaag caacagagca tctgtgcaga cagcgagcga
     6121 gggacaccgc tctcttggga attcttcact ccaccatcca gtttgcgcct aaatgccaat
     6181 gagggattta atattcattt aaaaccattc catggtcttg attataattt tcccccactg
     6241 catcagttat tttctctcct tcatctgtta agctcgttct aaaatataac ttcagagaga
     6301 actatgaact taaaagaata ccaactcaaa agagccaacc tacttctgca agcgccaaaa
     6361 tatcgtgttc tatgttctgc ctgtgctcaa cctgatttcg gttgttactg cgctcaagta
     6421 caaagcttcg atgcaaaaat taatttcgtt gtcctcatcc atcccgttga agtaaagcgg
     6481 cgtattgcca cgggtagaat gtctcatctc tgcttaaaag attcctactt gatcaaagga
     6541 caaaactata cggaagacaa tttggtcaat gaactcattc aagattccga acaccactcc
     6601 gtcattttat atcctgggcc tgcttcaaaa aatttatctc ttttacctac caaggagaaa
     6661 gccaatttat ttccgaaaaa taaaaaactg cgtattttcg ttatcgatgg gacctgggcc
     6721 acagcgcgaa aaatgatccg acaaagtgaa aacttaaatg tcctcccacg aatttgcttt
     6781 tccccagaaa agccttctaa tttccgcgtt cgtaaacaac cacatcctaa ttgctattcg
     6841 accattgagg ctattcatca tacgattgag ttgattggtg agtctcaagg tttttcagtt
     6901 aaaaaacgcg agcatgataa attactacaa gtgtttgatt atatggtcga aagacaactc
     6961 ggttttatcc gagaatcgaa tcttaattta agggttgcaa gttaccgccg ggaaggacaa
     7021 aagaagataa gttaatttta aattcttatc atttctccac ggggggatat ctgatgaaag
     7081 agtccaggaa ctcatttgac tgggaacgaa cagtcatcct cgagctcaat cgcgagcacg
     7141 aatacacttg ccgcatccac cggataaatc tccgtccgat ctccatcctt cttttcgact
     7201 cagcctctct ctggggccaa tttgataacc gaaccagaac aatctccatc tccagaaaac
     7261 ttgtgaatga atactcttgg caccacgtgg tcggagtcct tcgccacgaa atggcccatc
     7321 aatatattgc tgagaagagt cctttggaat tcatttctga aaacccccac agcgctttat
     7381 ttaaagaagc ctgcaaacgc ctcggcgttc ccgatcaatt tgccaaggct ggcatcaacc
     7441 tacagagctg caatcttgac tggaagacag agccgaaaga tgaagccact gagaaaattc
     7501 ttgataaggc aaaaaggctc ttggcactcg gcacctccac caacgaacac gaggctcatc
     7561 tagcaatgga acgagtccgc gaactttacg caaaacacaa tcttgaacac atggaaaatc
     7621 tctctcagga aaatttcgtt catctggtaa ttactcacgg aaagaaaaga gcggaatcat
     7681 gggagctgcg aacaatcagt atcttgaccg aacatttctt tataaaggct ctgacccttc
     7741 aacagtttga cgcgaactca ggtaaaagat ttcatgcggt cgagcttatt ggaacgcgag
     7801 aaaacgtaac aatggccgaa tatgtgtacc acttcttatt gcagcaagtg gagttcttac
     7861 taaggcaagc gagtaaccgt gaaatggggc ttgtacgcgc ggagagaact tcattccggt
     7921 tgggagtact ggatggcttc gataaaaagt tgaagctatc aactcaaaat atcccaaaag
     7981 actcccctga tgaaaataac aaagagcaaa cagtcattgg ccgcgccctt acgaaattcc
     8041 gggcggatcc ttatcttgag gactatctct ctgatattta cccgcgactt ggacaccgtc
     8101 ggaggtcgtc gcttgaaata gatggcaatg ccttcgcggc aggtcacgct gcgggaaaat
     8161 ccatcaatct taataaaccc attactacca atatggggaa tcagggaaga catcttggtg
     8221 gtggcaaatg aatttggttt ttactgtgga agatcgttaa cccaaatttt aattggaatt
     8281 gttcttggcg ctggaaacat aaatgatatt ggaaaagggg gagaagatga tttgattgcg
     8341 ggatttttta tttttccgaa tatccccaag atcaatatct gtaaactcat ttgaagtcca
     8401 aattatcgct gagcaattta tcttaccgaa tgttcgaaat gtggaaaaac aaattagatt
     8461 tcttgactgc tgagttcata cgaagcagcc tctaatccgt aaatcaaaga atatccgact
     8521 ttggcggatc caaaaattcc gagacctgct aagaagctct agaatttata aggaggaaaa
     8581 tctatgctgc gaatagttca aatttttgga ttagtttttt ttatatcgca ttttactata
     8641 gcatcagttg aagatcctca gtttttatta ccaaaagtcg acgcttttct ggcgaagcca
     8701 tcttttgcga gtgcctttaa ggcaggggac aaaatcaagt acgtaacttc tgattgtatt
     8761 ggtgagcgtc cttgcggcag cggagtgagc atgacggccg ttatagaccc tatctcaagt
     8821 gatgagattt gtattaatca atttgggcct gacaattcac ttaaagcgaa agacaaggtc
     8881 aatttacagc aatgggataa tatcaacaga aattatttgc gcgcgaagat caataaaatt
     8941 gaaagcttcg gcttcaaggt tagcgtcact agatttgaga acataacatg tcctcaagaa
     9001 gtctatcttg gacctgagtc tcaatgtgca aaggtatctt tgcaaggtgt aaatggttta
     9061 aataatacca gtgaatatca atacattttg tggaattcgc cgataggcat cgcccaaatc
     9121 atttcctcga cccaaacaga tcatatgcta ttcactcgaa ctatttctta ccgagtgacc
     9181 gaagtttata gataaatatc caaaaattac attggaggcg ccgatttcac agggaccttt
     9241 tcactgtgca tatgggccga gaatacctta actcatcaaa gacagtaaac caattgatcg
     9301 ttaaggcttg cctttgaata cgacatgcct gttcctaagc gatcaatata ttgaattttt
     9361 aatgtcgaaa tattttttag atttatgaaa aattgatatc cctcatctga actataacct
     9421 ggaaattccc tatgtcgata gattaagacg ggcgtctgtt ttgagaaatt cattttggcg
     9481 taaaaaacga tagcgctatc ataagccact gtttcaggtt tgccggttaa acactcaaaa
     9541 tcaacagtcc tagcgaaaat gactgtgggc aaagttaagc ttgttataaa tgctactaaa
     9601 aacttaaaca tagtcgatcc cttctttata aagcttgcag caagatctaa cgattattaa
     9661 taatttgttc aaccagaatg agtaatctcg gcagatggca ggaacttttt ataaaaagat
     9721 ggctaaggcg gataaagtgg ccgtgaagtt caatttctca cgacgtacat ggtcttgtaa
     9781 aacgagctgc acaaccagat cttccatcac gtccattttt cgagctattt cgatagcccg
     9841 acggatagat ggaacttttc gatttccgaa tgcaggccgc agtctcggac gggacccttg
     9901 tccgcaattt cgcagtccca tggtctagga gtaaattgca aagtttgagg gacctttaga
     9961 aggtctgtca ttattgttaa atggaagttg agataaagcc cacagggatt gccgtggaga
    10021 ttagctgtct aagatgtggg acaaaggcat tgatttgcaa aagctgttgg cgcaaccaac
    10081 ggtattgctc aacggtgtgt tcccgcgaag caaagcttga gcgccatcgt cgcgatcaga
    10141 agttatatcg tatgagtcct gaagggcgag aacgaaataa ggttcatcaa agagcctatc
    10201 gtcagcaaaa aaaaatccaa gagtgatcta tatacaaaag ttaaagaaaa tctgggacaa
    10261 ccccaagtgc gtgagcgttg ctgctgcatt tgtggtggta ctgtggaagt agtcttgagt
    10321 ctacagggcc cacgcagtgg gtattattca ttgcggagga attatcatgg caatgttgtc
    10381 ggcagagatc cagtcacaga ttttggttct tcatttttct gagaagcggt ccatgaaatc
    10441 tattgccagg gaatttggta ttgatccaaa gacggtgcgt cgagtggtta gacgtcgtga
    10501 agtcaggctt agcgccaagg tgggcaaaag gccaagtatt ttagatccgt ttcatgaaga
    10561 gatttgcgtg cagttgaaaa agaacccacg tataaccggc accgcgatga tgaattactt
    10621 gcgaacgcgc ggctatacgg gagggctttc gttagtacag cactacatcc agcatgagcg
    10681 gggtcgcttt gcaaaaccta gagaggcttt tttacgatta gagttcggtc cgggggaagt
    10741 ggcacaagtc gactggggcg agttcggaga tgtttttggt gatggagtta aaatccactg
    10801 ttttgcaatg gtgctagcgt actcgcgaat gatttatgtt gaatttacgc gatcggaaaa
    10861 gttcgaagaa tttatacggt gtcacgagca tgcgttcagg ttttttaacg gagtgccgag
    10921 agagtgttgg tacgacaatt tagccacggc cgttagtgac agaatgggcg ggattatccg
    10981 gttcaacgcc cggtttatgg cttatatggg tcatcatagt attcgtccgc acgcatgcaa
    11041 tgtggcccgt gggaatgaaa agggccgagt tgaggattta attaaataca tccggatgaa
    11101 tttttggggt ggacgtaatt ttacggactt cgaagatctg acaaaacaat ttatagtctg
    11161 gcgagatcag atcgccaata atcgcgagca tcgaacaacc cgaagagtgg tgcgactttt
    11221 gtttgagacg gcagaaaagc cacagttaat ggctttaagt ccagtcccgt atgatacgga
    11281 cgagatattt tctaggcagg tggcatcgga cttccatctt caatacgaga cgaaccgtta
    11341 ctcagtaccg tggacattgg ttgggatgac gatcacggtt cgaactaata gctatgaaat
    11401 aaaggtttac tacaacgaga agtttatctg ttctcactcg agaagttatt taaaaaacaa
    11461 agtatttacg acggaagtac atcgcactgg tcttttagag cgaaagcctg gggctacgcg
    11521 cgagacttgg caacttggtt atgtaaaagg ccttgggccc aggatggcag aatatgtaga
    11581 gttggtgcgc agaggtcctc ggtctttgaa gtacgagctc tcgcgcctgg tggcgctcgt
    11641 tacggtgtac ggtgaagaga tggtgcttga tgcttgtgct gagtgtttag gatctgggat
    11701 cgtgggtgtg gataacctgg aattattttt aaagcgtagc catcacccgt caaagacgaa
    11761 tttaaatcca gagccaatca aatttaacag tgaaaaacta aaccgggtcc atccggcggt
    11821 cgatcttcga aaatacgacg cgctctactt cgagggggaa aaccaagtga gtgcgtcgaa
    11881 agaagagaca aatggaaacg ataaaccaag tgctgcaagc gggccttacg gacctgaagc
    11941 taaaatactg gggccaagcg atgagcgagg atcttgggaa gatgaaggat caggagcggg
    12001 agatcatctc aaaatacctg agccaatgga tccaacggga aaagtcggag cggcacacgt
    12061 cgatgatcca gagcaaaatc agaacagcga agttccggcg ggtggaaacg gtcgagaact
    12121 acaacttccg tcactcgaag atgacggaga agatcgagaa aacgtatctg gcgctgcaca
    12181 acgggatcaa taaggacaat ttaccgtcgg cggtctttac gggacacgct gggaccggaa
    12241 aaactcatct gtcgcgggca cttggttatg ccgcctgcca gaagggatta tcagttttat
    12301 ttatcacggc ggcggagatg gtgaatcacc ttcagcatgc ccagaaaacc tacaacttag
    12361 aagcggaact aaataaatac cgtaaacctc aggtgttaat catcgacgaa ctcggttacg
    12421 tggcattgga cacgcaggcc agcaacttgt tcttccaggt gatctcaacc cgacatgatg
    12481 cagagcttgg taccattgca actaccaact tgccgttcgg aaagttcaac cagatctttg
    12541 ctaacgatgc catagcccat gccatagtag accgcctcgt caatgaggct gaagtgtttt
    12601 acatggaggg tgatagctat aggccacacc aaagacaaga aaaattaaag cgaaaaaaac
    12661 aaaacgctta atcactcgat aaaaaatcgg ggtggcattg gctcaattaa atctcgatgg
    12721 ccaaggccac ctcatatctg atcgtaaaaa gtccctttac agaatgcgtg cttcagaccc
    12781 tggaggactg cgaaaatgcg cgaagaggtc cctctcgaga ctgcagcgtg cacttgaaaa
    12841 tgcgaaagaa gaaaaacaag cccacggtga aatcgtgacc taccatcccg gctttctatt
    12901 ttgccaagac acttacttgt agaagttagg atttgatctg acaggcgccc ttttattcag
    12961 caaatttatt tgtactgtca gttcatattc aagccgatgt ctttttgtct ccagcgacga
    13021 aactttgact ccgcgacagg atcaacagct tatttgacag gtttataacc ctcaaatttt
    13081 tcgttttcac cattgtctgc aattttattt ttgcctccta cttcaacttg tggtggcaaa
    13141 taatatgctt tcttaacgta agttcctatc ccaatttcat tatgttgaat agtgacatca
    13201 tttaaactga gaaatgagga atccttagcc gccactccat aatgggactc attaagtaca
    13261 gaatgcacaa cgaaagctcg tgagttctct ccgactgaaa tatttttatc tgattggtga
    13321 tccatattcg agttttcgac gaaagcaaga gtgccagaaa tatccaatcc gtctcccaga
    13381 ttgtttcgca gtaaaagatt atttccctta aaaacacaat aatcacaatc aaaagcatca
    13441 ctggcagatc cttcaattct gatgctcttg gcttcaacat tagacttgta gacattgaga
    13501 gcgtcctctg tctgattgtt catcaatact gaattttcaa tttgaaactt tcgaacatta
    13561 aaaatcgtca tggctccagt atagctgagc ccagcgcagt cgacggtttc atttccatcg
    13621 gtttttaatg ttcccacacc cgtcatgtaa ac
//
LOCUS       DATBTE010000174         1922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_227146, whole genome shotgun sequence.
ACCESSION   DATBTE010000174 DATBTE010000000
VERSION     DATBTE010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227146"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VIG33_06530"
     CDS             complement(<1..196)
                     /locus_tag="VIG33_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270527.1"
                     /translation="MQKKSVTESSIKKMTGNKRTVPVSVDLSLKEKNTRKYIDSPSPS
                     SIKASVSKQEAQWNWRQLRMI"
     gene            496..1185
                     /locus_tag="VIG33_06535"
     CDS             496..1185
                     /locus_tag="VIG33_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8270528.1"
                     /translation="MPKVLVIEDNEEAHFLLQRILATSHEFTIVSSLQSAIEHIEKEN
                     WDLIILDRDLPDGDGLSLCLKLKSLALNFQPFILMLTAYSELEDKINGLLAGADDYVV
                     KPFEPRELLARMDALLRRKPSGPDVSSTVSLGDLTINLEKHTMSVKKSLEETISFDLT
                     PIECKVLLTLVKNYGQEIGRDQLRQIVWDKVHLSIRNIDTHICHLRKKIAPSNFLIKN
                     RRGKGYYLIKK"
     gene            1214..1507
                     /locus_tag="VIG33_06540"
     CDS             1214..1507
                     /locus_tag="VIG33_06540"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY8270529.1"
                     /translation="MKSIKTEIDIDADIIEMVPDFCQARNADFENIAQYLKTKNFTAI
                     AEISHTIKGIAKPYGFPTLEKLCRELELAAKAKDENKCLHVFHQIKEYFIPFM"
     gene            1641..>1922
                     /locus_tag="VIG33_06545"
     CDS             1641..>1922
                     /locus_tag="VIG33_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8270530.1"
                     /translation="MIQFPAGPNHLIFTEHKKTTSQDDQLQLSGVKILLVDDSRESLV
                     IISHLLRRVGALVDFALDGEKALECNASTVYDIILMDISMPVMDGIEATR"
BASE COUNT          628 a          338 c          391 g          565 t
ORIGIN      
        1 gaatcattct taattggcgc caattccatt gagcttcttg tttgctcacg gaagctttta
       61 tggaagaagg cgatggagag tctatatatt tcctggtgtt tttttctttc aaacttaaat
      121 ctacggacac cgggacagta cgtttatttc cagtcatttt tttaatcgat gattcggtga
      181 cagatttttt ttgcataggg caaaatgctc cttattaaaa atagggatag gatcaaatct
      241 aatcgcatat acctgagaga ttttttattg gcaaaaaatc taagcaattg ttaagaatat
      301 taaacaaacc tcccttgcaa agtgttgtga tttcttaaaa aagaagcgaa acatatacat
      361 cgtgagcggt tttcgagatt attctaccaa gatttacatg gaaatccgtt ttgtaatatc
      421 tggcgatatc taagaagttt ggaaaatgag tgatgaccat ccattaaaga tagatgctac
      481 aggggggagt aaaaaatgcc aaaagtactc gtcattgaag acaatgaaga agcacatttt
      541 ttactgcaaa gaatattggc gaccagccat gagtttacaa tcgtttcaag tcttcaatcg
      601 gctattgagc acattgaaaa agaaaattgg gacctgatta ttctggatcg tgacctgccg
      661 gatggggatg ggcttagtct ctgtttgaaa ttgaaatctt tggctctgaa tttccaacct
      721 ttcatcttga tgctcaccgc ttatagcgaa ctagaagata agattaatgg tcttttggct
      781 ggagctgatg attatgtagt aaaacccttt gaaccccggg agcttttggc acgcatggat
      841 gctcttctcc ggagaaagcc atcgggcccc gatgtctcat cgacagtcag cttgggagat
      901 cttacaatca atttagaaaa gcatacgatg agcgttaaaa aaagtcttga agaaacaata
      961 agctttgatc tgacgccgat tgagtgcaaa gtactactta ctttggtcaa aaattatggt
     1021 caagaaattg ggcgagatca attgaggcaa attgtgtggg ataaagtcca tctgagtata
     1081 aggaatattg atactcatat ctgtcattta agaaaaaaaa ttgcacccag caattttcta
     1141 ataaaaaatc gtcgaggcaa aggttattac ttgataaaaa aatgataatt tgtcttatac
     1201 aaagaggaac ccaatgaaaa gtatcaaaac tgaaattgac attgacgcgg atattatcga
     1261 gatggttcct gatttttgcc aagccagaaa tgccgatttt gaaaacatcg ctcaatattt
     1321 aaaaacaaaa aattttaccg ctattgccga gatcagccac actataaaag gaattgccaa
     1381 gccctatggt tttccaactc ttgaaaaatt atgcagggag cttgaactgg ctgctaaagc
     1441 taaagatgaa aacaagtgcc tccacgtttt ccaccaaatc aaagaatact ttatcccatt
     1501 catgtaaata aataatttag taaaatgatt ttaatttgag tatgagaccc gaagtgttga
     1561 gtttttttaa aacttgatta ttcgatagtt aacttcaatt taaaagtagt aaggtaagga
     1621 tattaaaaaa gggaggagct atgattcaat ttccagcagg ccctaatcac ctcatcttta
     1681 ctgagcacaa aaaaacgacc tcacaagatg atcaattaca actgagtggc gtaaagatcc
     1741 ttctcgtcga cgattctcgg gaaagcctgg ttataatcag ccatcttttg aggcgcgtcg
     1801 gcgcacttgt cgatttcgca ctcgacggcg agaaggcctt agagtgcaat gcgagtacag
     1861 tctacgacat tattcttatg gatatttcaa tgccagtcat ggatggcatt gaggcgacaa
     1921 gg
//
LOCUS       DATBTE010000175         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_432235, whole genome shotgun sequence.
ACCESSION   DATBTE010000175 DATBTE010000000
VERSION     DATBTE010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432235"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..763)
                     /locus_tag="VIG33_06550"
     CDS             complement(305..763)
                     /locus_tag="VIG33_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEY8270531.1"
                     /translation="MTSNFAEEDQVRTLYKDLLHQWNEQKAQGMANLFMHNGSLVGFD
                     GSQVNGAKEIEAHLKPIFASFPTARFISIVREVRGLSAETALLRATVGMIPRGHFDIN
                     PAVNAIQSLVAVKQNGQWRVAHFQNTPAAFHGRPEEAEKLSKELRAVIFN"
BASE COUNT          426 a          334 c          308 g          453 t
ORIGIN      
        1 ttttcttcaa gtcgaactgg tttacagagg gagaatctaa agtctccgcg atctcgtgct
       61 accgaaacac taaattctaa tccgtagctt gcttcgcaaa ccacggatta tctgacgata
      121 taaatttcat caaatcaaaa atgcctgggt tcctgatttc ttattgccgc ttattcaact
      181 ctaaccatag acggaatccg atcttttctt ttagacctta tagtcgattt aaattgtcga
      241 aggcgatcac taattgttgg tgtgggaaaa cgggccgcca cctagtgctg atctaaaatt
      301 actttcaatt aaaaataacc gcgcgcaatt ccttgctgag tttctcggcc tcttcgggac
      361 ggccgtgaaa agcagcggga gtattttgaa aatgcgccac tcgccattgc ccattttgct
      421 tcactgccac cagagactga atcgcattca ctgcgggatt aatatcaaaa tgtccacggg
      481 gaatcatgcc gacagttgcg cgaagaaggg ccgtctctgc ggataagcca cgcacttcgc
      541 gaacaatgct aataaacctc gctgtcggaa aacttgcgaa aataggtttg agatgggctt
      601 caatttcttt tgctccattg acctggctgc catcaaaacc cacgagactg ccattgtgca
      661 taaaaagatt cgccataccc tgagcttttt gctcattcca ttggtgtaac agatctttat
      721 aaagagttcg tacttggtct tcctctgcaa aatttgaagt catcttttac tcgccttgtt
      781 cataaatagt tttatttgaa ggagccttag atgattttat aattccttat cccctctata
      841 aaaacaatta ataagacaag tggctaaaag ccagatcttt actcacgcgc ggagttctcc
      901 ttaggtggga tgtggaggtg ggctttgagg gtcgcgcgat ctgcgcagtg ttttagagac
      961 ggcccctttt ggagccaaga tggagaaccg ggtttcaaga gattttctct ctggaaaaga
     1021 aataccaaaa tagttcccct cttggccaat cgattgatat ttcagcaggt ataagaaaaa
     1081 ctaatgcttc gctctgtcaa aactttctct cttagacagc ggtttaggaa agcatcttaa
     1141 tttgaaacgc tgagaatgtt ctatttttcg tataaaaagt tttcaaaaga gactttagtc
     1201 tcgtcataaa atgccgatat agtaaatata cggtatgtaa ctggcgaggg atcaaatgct
     1261 tagatatttt acaactttgt ttacctgctc attccttatg gcctcctcat cggccgttgc
     1321 tcaaagtcaa agtcagattc tcgatacgaa gggcaacctt atacttacac agagttatga
     1381 cttaggctta caaatgacgg ctcaatgtaa gtcttttcga atttctaaaa accctccact
     1441 cacaccacga cttaagtgcc gtttttccta gggttaggat aatcttgggc tcccaacaga
     1501 ataaaattta atagtcgggg a
//
LOCUS       DATBTE010000176         5614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_978770, whole genome shotgun sequence.
ACCESSION   DATBTE010000176 DATBTE010000000
VERSION     DATBTE010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978770"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(393..812)
                     /locus_tag="VIG33_06555"
     CDS             complement(393..812)
                     /locus_tag="VIG33_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270532.1"
                     /translation="MKKVFYIMSVLSLSFSAIAANQGKMIQVQGKDGVQLELELVYDN
                     AQVNPEGKLVIEGPMVALKKKMAADRKVMLDYNDVTATALCQQLGYSKGTAKETSETT
                     PFLGNYELISANNADKKLTLYVQADSDSIRKGTIVCQ"
     gene            complement(1248..3479)
                     /locus_tag="VIG33_06560"
     CDS             complement(1248..3479)
                     /locus_tag="VIG33_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY8270533.1"
                     /translation="MNHLPPMIIDLVLILGAAAVGTLVFSKLRQPIVLGYLIAGFAVG
                     PHFPLWPTVADGEGVKLWAEIGVIFLLFGLGLEFSFKKLAKVGKSAFIAATFEIITMS
                     TVGYLFGQALGWTTMDSIFLGAILSMSSTTIILRAFAEAGLKSKAFASLVFGILIVED
                     LMAILLMVILSAISVPGSFSGSNLVYLSSRLVFFLLAWFLVGIYILPTLLQRIRRQLS
                     NEIMLIVSIALCFLMVVLATKANFSAALGAFIMGSILAETEEGERIEHLLISVKDLFA
                     AVFFVSVGMMIDPYILREHFGVVVLITIITIVGKLIGSGFGAVISGRSLKHSTQAGMS
                     LAQIGEFSFIIATLGVSLNVVSDFLYPIIIAVSAVTTFTTPYMIRNSDPIYSWIERRL
                     PQTFLDRLSQYERALSANGKESALGLLWRTYGLGILLNSVVVLGISLAVSTWVLPFIL
                     STFGDTSAVRFFSCVLTLVACSPFLSAVIFKTPKTIRSEEAESILRIRRLQVGVTMVR
                     AVIGLVLIEGIISQFARSTILPLITLAVTPILIFIFRGRIGRFYSIIEDRFLKNLNAK
                     EISEMESLAKLPQLAPWNATLAQIVLTSESRLAGHTLEEGKFRTETGATVGMIDRGSR
                     RIFAPGGSERLLPGDELFLIGTEEQIAAARELVHPKESQVSLPHDELYSLESLSIEDI
                     SPYARKSIREVGFGEQFGGLIVGIERGGNRILNPESTVVLEPGDLVWIFGHEMKIRAL
                     KRS"
     gene            complement(3596..4018)
                     /locus_tag="VIG33_06565"
     CDS             complement(3596..4018)
                     /locus_tag="VIG33_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270534.1"
                     /translation="MTAKITAFVFTILGSFPLWVSAYQDNGTNKTCTGTVLVVMGENS
                     APLDMSLALIRSAGSTQGQFTMTVFELFVSGAYTVNSKDFLEASFEIPDKTVTTTGSI
                     HQIDKDSVGFTATMMDKSPDGSTKSLSLLAPNLTCVNN"
     gene            4242..4760
                     /locus_tag="VIG33_06570"
     CDS             4242..4760
                     /locus_tag="VIG33_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270535.1"
                     /translation="MNRIFVLIALISFTAFSANAEEATEDIMQWTTSEVSVTPTRPGS
                     CQSSAHVGVNRIFEVEIYENDSLILVSFKTEHGPCGHRETMNNPHVKIIGDAFYSLFD
                     TYPAEGTIYVSNVNPALGLVNLTFDKKRIFKNSSVATFAYSLILDQGYEFRWNVKLTQ
                     LLDHTTLLIVGM"
     gene            4820..5131
                     /locus_tag="VIG33_06575"
     CDS             4820..5131
                     /locus_tag="VIG33_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270536.1"
                     /translation="MNRTYLRSIGLSLTIITLTSLSWGHTRLPLESEIAPDCVNLGYF
                     EGTAGYGKSLDGRRIALYRAQRAAAEAGANFTIVLQLDRGLSDQGGYCLLKAYHCLTL
                     K"
     gene            complement(5189..>5614)
                     /locus_tag="VIG33_06580"
     CDS             complement(5189..>5614)
                     /locus_tag="VIG33_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEY8270537.1"
                     /translation="DLLKRVDIVPLPMLLAQAALESGWGTSRATRELNNLFGMHASKG
                     QPCQMGFDSRTACIRKFSSITESVSAYLRLLNVGVYYSQFRERRAQMREAKEALDSIK
                     LLQALGKYNVTPTAYVQKVRDIMTKSSNLTQFAFKEEEN"
BASE COUNT         1671 a         1277 c         1174 g         1492 t
ORIGIN      
        1 tgaagtctct attaagtgtt cgccatagag aacatgagag cgaataaaag cgtttttcta
       61 gtgaggtccg ttcccgcgat taccaattgc aaatggggat cagcgatccc agaatttcat
      121 tacctgagtc ttttgttctg ccatccttgt ttaggatccg ggtctactaa aaatggttgg
      181 atccctagcc ataatctttt agttgtttac gcaagtcaaa tcagacgcaa ctagagtgag
      241 cgacttggta cttccatccg gagatttatc aatcattgtt gctgtaaatc caatactgtc
      301 tttatcgatc tgatgaatag agccaaagaa gaaaaagaaa aacgccactg gtctgattgc
      361 cagcggcgat gatttcaact caacttcagc aattattgac aaacgatcgt gcctttacga
      421 attgaatccg aatcggcttg aacataaaga gtcaactttt tatcagcatt attggcactg
      481 atcaattcat aatttcccaa gaaaggtgtt gtttctgaag tttcctttgc agtcccttta
      541 gagtatccca actgctgaca aagtgcagtt gcagtcacgt cgttatagtc gagcataact
      601 tttctatcag cagccatctt ctttttaaga gcaaccatag ggccttcaat aaccaactta
      661 ccttcagggt ttacctgggc attgtcatat accaattcaa gttccagctg caccccatct
      721 tttccttgga cttgaatcat ttttccctgg ttggcagcta ttgccgaaaa actaagactc
      781 aaaactgaca ttatataaaa aacctttttc ataaatatcc ttctcgcgtt caaaatattg
      841 tgggattcat ttccctttat caactgaacg gctcctttgg atgacggagg ctgcgagaac
      901 catacccgtg taaataggtc tatatttgat ctgagaaatt ggagatgaat agaatataga
      961 cgcactttga aatgaaaagc aaaggatcga gcgagattcc gaggatttag aaaaaatatg
     1021 acattttttt tctcagagcc aataatgggt attgcaaggc ggcctctccg ttttttagaa
     1081 tggggtagtt tgtgccaagt cctccggaat gctccaaaat caccccttgg tgtttatata
     1141 ctttgaaggg ctcagctctg attcgcgaac caatcggtac cacaacacta aggacttttg
     1201 aaattcaatg acattcgtct tatcaagcat ccggtgaaaa tgttccacta ggatcgttta
     1261 agggcacgaa ttttcatttc atgcccaaat atccagacca aatcgccagg ctccaagacc
     1321 acagtgcttt cgggattaag aattctgttt ccgcctctct ctattccaac aatcaggccg
     1381 ccaaattgtt ctccgaagcc aacttcacgg atcgattttc tggcgtaggg agaaatatcc
     1441 tcaatactca aggattcgag agagtaaagt tcatcatgag gtaaggaaac ctgtgattcc
     1501 ttggggtgaa ctagttctcg agcggccgca atttgttcct ctgtgccgat aagaaataac
     1561 tcatccccag gcagtaacct ctccgatcca cccggcgcaa aaattctacg gcttccgcgg
     1621 tctatcatac caaccgttgc acccgtctca gttctaaatt taccttcttc caaagtatga
     1681 ccggcaagtc gagattccga ggtcaaaaca atctgcgcta gcgttgcatt ccaaggggca
     1741 agctgaggca actttgcgag cgattccatc tctgaaattt ccttggcatt cagatttttg
     1801 aggaacctgt cctcaatgat tgaatagaaa cgtccaatcc gccctctaaa gatgaaaatg
     1861 agaatcgggg ttaccgctaa agtgatcaag ggcaaaattg tcgatcttgc gaactgacta
     1921 attatgccct ctatgagaac caagccaatc acagcacgga ccattgttac gccgacttga
     1981 agcctacgta tgcgcaaaat ggattccgct tcctcgctcc gaattgtttt aggagtctta
     2041 aaaatgacgg ctgaaagaaa aggcgaacag gcgacaagcg ttaagacgca agaaaaaaat
     2101 cttaccgccg atgtgtctcc aaaagttgag agtataaacg gcaataccca tgtagatact
     2161 gcaaggctaa tacccaatac caccactgaa tttagtaaga tccctaaacc ataagttctc
     2221 cacagtaagc ctaaggcact ttctttgccg ttggcagaca aagctcgttc atattggctt
     2281 aagcgatcaa gaaaagtttg aggcagacgt ctctcaatcc agctgtatat gggatctgag
     2341 ttgcggatca tgtatggtgt tgtaaatgtg gtcaccgccg aaacagcgat gatgatgggg
     2401 tataggaaat cactcacgac attcaggctc acaccgagtg tcgcgatgat aaatgagaat
     2461 tcaccaatct gagccagact cattcccgct tgggttgagt gtttaaggct tcgtccagat
     2521 atgacggcgc caaaaccaga ccctatcagc tttcccacaa tggtaataat tgtgatgaga
     2581 accacgactc cgaaatgttc tcgaaggata tacggatcaa tcatcattcc gacagagaca
     2641 aaaaataccg cagcgaaaag gtccttaacg gagattaaaa gatgctcaat tctctcgcct
     2701 tcttcggttt cggctaaaat agaacccatg atgaatgcac ccaaggctgc cgagaagtta
     2761 gccttagtcg cgagtacgac catcaaaaaa cataaggcaa tcgatacgat cagcatgatc
     2821 tcattactaa gttggcggcg tattcgttgg agcaaagtcg gaagaatata gattcctact
     2881 aaaaaccaag ccaagaggaa aaacacgagg cgagaggata aatagaccag attggaacct
     2941 gagaatgatc ctggaacgct gatagctgac aagatcacca tcaaaagaat cgccatcaaa
     3001 tcctcaacaa tcaaaatgcc aaaaacaaga ctcgcaaatg ctttgctctt tagtcctgct
     3061 tccgcaaagg ctcggaggat aattgtcgtc gacgacatcg aaagaatggc gcctaagaaa
     3121 atgctatcca tcgttgtcca tccgagagct tggccgaaca gataacccac agtgctcatt
     3181 gtgatgattt caaacgtagc cgcgataaaa gcactctttc ccacttttgc gagcttctta
     3241 aaactgaact caagccctag gccaaaaaga agaaatataa ctccaatttc agcccacagt
     3301 ttgacccctt caccgtcggc aactgtgggc cacaagggaa agtggggacc cactgcgaat
     3361 cctgcaatga gatagccaag gacgatcggc tgacgaagtt tgctgaaaac cagagtaccg
     3421 actgcagcgg ctccaagaat gaggaccaga tcgattatca ttggaggcaa atgattcata
     3481 aggtgctcca agttagcttc aactacacag cgacgctatg tggttaattg aaaaaaaacc
     3541 atcctcgagg tgcgggtacc gacctcataa tagttgaatc cctagccatg accctctagt
     3601 tgtttacaca agtcaaatta ggcgcaagga gagagaggga cttggtactt ccatccggag
     3661 atttatccat cattgttgct gtaaatccta cactgtcttt gtcgatctga tgaatagagc
     3721 cagtcgtagt tactgtttta tcaggtatct caaatgaagc ttcaaggaaa tccttactat
     3781 tcaccgtgta agccccagaa acgaaaagtt caaaaacggt cattgtaaat tggccttggg
     3841 tgcttcctgc ggagcgaatc aaagcaaggc tcatatccaa tggtgcagaa ttctcaccca
     3901 tgacaactag aacggtgcca gtgcaagttt tattggttcc gttgtcttga taagcgctga
     3961 cccagagagg aaagcttcct aaaattgtaa aaacaaatgc agtgattttt gctgtcataa
     4021 attgatctcc tggggccgaa atatttcggc aatttaaagg caaatttgca gtaaattgct
     4081 ttatcaatac cttaaagtca agaagtctaa aacctttgag agccctgttg aaaatcaggc
     4141 tttgagataa attttagaga agaactgatt ttgaaccaac cagttctatt gactaattat
     4201 acagccaaag ctagatcatg tcccagcaaa ggaagtatag catgaacaga atattcgttt
     4261 taatagcctt aatttcgttc acggcttttt cggcaaatgc ggaggaagcg actgaagata
     4321 tcatgcaatg gaccacctct gaagtcagcg tcacgcccac tcgtcccgga tcctgtcaat
     4381 cctctgccca tgttggcgtg aatcgtattt ttgaagtgga aatttatgag aatgactcgc
     4441 tgattttggt aagctttaaa acggaacatg gtccttgtgg acacagggaa accatgaaca
     4501 accctcacgt gaaaattatt ggtgatgctt tctatagttt gttcgacaca tatcctgcgg
     4561 aaggcaccat ttacgtaagc aatgtgaacc cagcactggg cctcgtgaat ctaacctttg
     4621 ataaaaaacg tattttcaaa aacagttccg tcgccacttt tgcctactcc ctcatcctgg
     4681 atcagggata cgaatttaga tggaatgtga aattgactca actccttgat catacaacac
     4741 tgttaattgt cggaatgtaa actaaagctt tgaaatttat aggttctcgg tttaagcccc
     4801 tcctaacaag gagtaaacta tgaacaggac ctatttaaga tccatcggcc ttagtctgac
     4861 tataatcact ttgacttctt taagttgggg ccatacgaga ttgccactgg aatcagaaat
     4921 tgcacctgat tgcgtaaacc tcggttattt tgaaggcact gccggttacg gaaaaagctt
     4981 ggatggaaga cgaatagccc tttacagggc acagcgcgcg gccgcagagg ctggagctaa
     5041 ttttaccatt gtccttcagc tagaccgtgg gctctctgac caaggaggct attgtctctt
     5101 aaaagcttat cattgcctaa ccttaaagta atttaatcgg caaatatttt tgtttccatg
     5161 caattccaag gggaatacat ggacaccttt aattttcttc ttccttgaaa gcaaactgcg
     5221 tgagattgct cgacttcgtc atgatatcac gaaccttttg aacataggcg gtcggagtta
     5281 cattatattt tcccagagct tggagcaact taatactatc caaagcttcc tttgcctctc
     5341 tcatctgagc tctcctctcg cggaattgcg aatagtaaac tccgacattt aagaggcgga
     5401 gatatgccga aacactttcc gtaatggaag agaactttcg gatacaagcg gtgcggctat
     5461 caaaacccat ttgacagggc tgccccttgc tcgcatgcat gccaaaaaga ttatttaatt
     5521 cacgggttgc acggctggtt ccccaaccgc tttctaaagc cgcctgggcc aataacatcg
     5581 gcaaaggaac aatatccact cgttttaaaa ggtc
//
LOCUS       DATBTE010000177         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_705758, whole genome shotgun sequence.
ACCESSION   DATBTE010000177 DATBTE010000000
VERSION     DATBTE010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705758"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VIG33_06585"
     CDS             complement(<1..321)
                     /locus_tag="VIG33_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HEY8270538.1"
                     /translation="MSQIKMIKNLFPSPIYYAPLVAKGFKQLNHELLDESLKFRKIDD
                     AGIKWSEYNYRGGYTSYGSIAHLHQVSTTFEELRKEIDKHVRKYIKYLEMDVNPKQIQ
                     MCSCW"
     gene            505..843
                     /locus_tag="VIG33_06590"
     CDS             505..843
                     /locus_tag="VIG33_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270539.1"
                     /translation="MKTVFLSLFVVMTLPLSSFAKVEDFNAMINESEHAQTQLRKDLK
                     GHIGSTHQAQMNAPKEIIEVASEYGESVNSPTKKSMLTFEKEMVHYRPSETRRMKRLA
                     NEIKASDKAF"
     gene            complement(926..1462)
                     /locus_tag="VIG33_06595"
     CDS             complement(926..1462)
                     /locus_tag="VIG33_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY8270540.1"
                     /translation="MASSTVRYSLNKNKYLLPPEVERLKKIISDYQDKDARNCLLIDL
                     GLRTGGRAQELLNLTMADLNTYDESVFIRGLKNSNDREIPLHSEFFHRLHKFASQQDG
                     RHLFGISYNRLYQIWELYRPVPKKFHSLRHTFAIELYQKTKDLRLVQVALGHRNITNT
                     MIYADYVYSQQELKKLIL"
BASE COUNT          491 a          316 c          354 g          473 t
ORIGIN      
        1 ccagcaactg cacatttgga tctgcttcgg atttacgtcc atctccaggt acttaatgta
       61 tttgcggaca tgtttatcaa tctcttttcg cagctcttca aaggtggttg aaacttgatg
      121 aaggtgggca atagagccat aggaggtata gcctccacgg taattatatt cagaccattt
      181 gattccggca tcgtcaattt ttctaaattt gagactttca tccaagagtt catggttcag
      241 ctgtttaaag cctttagcga ccaagggcgc ataataaata ggggagggga ataggttttt
      301 tatcatctta atttgagaca atactcaagg aaaacagcct atgcaagcgc gtgttcgtat
      361 ataaaacagc tgttatccaa aattggcagt ttatcaactt gagatattgg cacggttctg
      421 aactcgatta cagaaggctc cgggctggca ctccccttgc tctagtagtc agtaagccga
      481 ctgactaaaa aattggagga tcttatgaaa actgtttttc tatctctctt tgtagtgatg
      541 actctccctt tgagctcttt tgcaaaagta gaagatttca atgcaatgat caatgagagc
      601 gaacatgcac aaactcagtt acgcaaagat ttaaaaggac acatcggaag cactcatcaa
      661 gcccagatga atgcgccaaa agaaatcatt gaagtcgcca gcgaatacgg agaaagcgtg
      721 aactctccaa cgaaaaaatc gatgctgact tttgagaagg aaatggttca ttaccgccct
      781 tctgaaacta gacgaatgaa gcgcttggct aacgaaatca aggcttctga taaggctttc
      841 taaggtttgt ccaaaaaaat tgaatttacc cctaaaaacg gtcgacgatg tccccccatc
      901 tcggccgttt tccttttcct cggctttata aaattaactt tttaagttcc tgctgagagt
      961 aaacatagtc cgcgtaaatc atggtattgg tgatgtttct atggccgaga gctacctgga
     1021 ccagtcgcag atctttggtc ttttgataaa gttctatggc gaatgtatgc cggagggaat
     1081 ggaatttttt cggtacgggc cgataaagct cccagatttg ataaaggcga ttgtaactga
     1141 tgccaaagag atgtcgaccg tcctgctgac tggcgaattt atggagacga tgaaagaact
     1201 cggaatgaag ggggatttct cggtcattag agttctttaa accccgtata aaaacacttt
     1261 catcataggt gtttagatcc gccatggtta ggtttaataa ctcttgagct cgacctcccg
     1321 ttctaaggcc gaggtcgatg agtaagcagt ttcgagcgtc cttatcttga taatcagaga
     1381 tgattttttt gagtctttca acttcaggtg gcaaaaggta tttgttctta ttgagtgaat
     1441 aacgaaccgt tgagctagcc aaaatgaacc tcaaatatcg ataaaaaaac caggaaaaaa
     1501 gtgcgtattt catgattaaa aaaggcagga cctttgtaaa gaactgaggt gatcctgtga
     1561 ctattaataa atgcgttgcg ttatatctca aaacacattg agtcaattta aattatgcaa
     1621 aatggttaat acat
//
LOCUS       DATBTE010000178         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2414087, whole genome shotgun sequence.
ACCESSION   DATBTE010000178 DATBTE010000000
VERSION     DATBTE010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2414087"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..421)
                     /locus_tag="VIG33_06600"
     CDS             complement(137..421)
                     /locus_tag="VIG33_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY8270541.1"
                     /translation="MSMTIDISSAMRIQYIERLNNTLVDCIKAMEILDFCFLEKIGHQ
                     LKGNVLSFGFGELTSLAQALESAAILKDTLQLEIILKSLNLTVLTINHQL"
     gene            complement(440..1840)
                     /locus_tag="VIG33_06605"
     CDS             complement(440..1840)
                     /locus_tag="VIG33_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8270542.1"
                     /translation="MQFKAKIVLIDDDQDTLNLLSRFFASHGYETHVYKDALIAYEEL
                     SNARMNCDVIVTDLSLPGISGIEFTKQMKEAGNEIPIILITANRKVEVAIEAISAGAY
                     DFVLKPLHFPQLLVSIERALYLKRLLKDNQTLKAVVKLQEEPLGVEGVIGKSAGIKHA
                     IDLARRVSSSSANVLLSGESGTGKEVIAKAIHNLGSRRKSPFMAINCAAIPENLLESE
                     LFGHAKGAFTGASDKKIGIFEEADSGTLFLDEIGDLNLALQAKLLRVLQERKIRRVGE
                     NVYRPVKARIISATHKNLREAIELKTFREDLFYRLNVIPIWIPPLRERKEDIIPLAEF
                     FLKKYSALNNLRNRQFSKSCLEALSNHQWQGNVRELENSIERALVLSDSDIIHASDVI
                     SIDESDTRGHGIHAHFDYSKVEGSVILSMEELTNKYVRYVLELNHGLKEKTAKDLNID
                     RKTLYRRLRAIERPLN"
BASE COUNT          603 a          460 c          392 g          643 t
ORIGIN      
        1 ccccaagatc actctcctta tttccagttc agtgagatca gcgagaaatg taaaaccgcc
       61 ggctgtacag cttatttgac aaaaccgatt aattttaacc aacttattac attgatcacc
      121 aaaatattgc atgattctaa agttgatgat taatggttaa cactgtcaaa ttaagcgatt
      181 ttagaatgat ttcaagctgg agagtgtctt taagaattgc cgctgattcc agggcctgtg
      241 caagagaagt taattcacca aaaccaaaag aaaggacatt gccctttaat tgatgtccaa
      301 ttttttccag aaaacaaaag tccaggattt ccatggcctt tatacaatca actaaagtgt
      361 tattgaggcg ttctatatat tgaattctca ttgcactaga aatatctata gtcattgaca
      421 tggttcgttt ctccagaaat taatttaagg ggcgttcaat tgctctcaat cttcgataca
      481 aagtcttacg atcgatatta aggtctttgg cggttttttc cttgaggcca tgattgagct
      541 ccaaaacata tcgcacgtat ttatttgtta actcctccat cgacaaaata actgatcctt
      601 ccactttaga atagtcaaaa tgagcatgaa tgccatgacc gcgcgtatct gattcgtcaa
      661 tacttataac gtcactggca tggatgatat ctgagtcaga aaggacgaga gccctttcaa
      721 tggagttctc aagttcacgc acattgcctt gccactgatg attgctgagg gcttcgagac
      781 acgatttgct aaactgcctg tttcgcagat tattcaaagc ggaatatttt tttaaaaaaa
      841 attccgccag gggaataata tcttcttttc tttcacgcag tggtggaatc caaatgggta
      901 ttacatttaa cctgtaaaac aaatcttccc taaaggtttt aagttcaatg gcttcgcgaa
      961 ggtttttatg cgtagcggat ataatcctag cctttaccgg ccgatagacg ttttcgccga
     1021 ctcgtctgat ttttcgttcc tgaaggactc gtaaaagttt tgcctgcaaa gctaaattca
     1081 gatccccaat ttcgtccaaa aacaaagtgc cgctatccgc ttcttcaaag attcctattt
     1141 ttttatctga ggctcctgta aaagcgcctt tggcatggcc aaagagttca gattccaaaa
     1201 gattttccgg aatagccgcg cagttaatgg ccataaaagg cgattttctt cgtgaaccta
     1261 aattatgaat ggcctttgca atcacttctt ttccagtccc gctttcaccc gacaacaaca
     1321 cgttggcgct cgatgaggac actcgccgcg ctaaatctat cgcatgtttt attcccgcac
     1381 tctttccgat gactccctcg actccaagag gctcctcttg caatttgacg actgccttca
     1441 aagtttgatt atctttaaga agtcgcttta aatataaagc tcgttcaatc gatactaata
     1501 actgtggaaa atgcagaggc tttaaaacaa agtcgtaggc acccgcactt atagcttcta
     1561 tggccacttc aacctttcga tttgcagtga ttagaataat cggaatttca ttaccggcct
     1621 ctttcatttg tttcgtaaat tcaatacctg aaatacccgg taaacttaga tcagtgacaa
     1681 taacgtcaca gttcattcgc gcgttggaaa gctcttcgta agcgatgaga gcatccttat
     1741 agacgtgggt ttcatatcca tggctggcaa agaatctgct taagagattt aaggtgtctt
     1801 gatcatcatc gatgaggact attttcgctt taaattgcat ggaaagttat accatgccgc
     1861 tctagtagag ccctatggat taatttattt tatataaaat caattttaga tcagttttcg
     1921 cgtccctgga tagtcggagg ggccattcgg gtcctattaa ccgactgcgg aaggctcttt
     1981 ccgctctaat gttagattac atcaaataaa acgagaggcc aaggtggcta tgtttcgccc
     2041 agccaccatc cctcaaaact ctcccaataa tcaaaataac caatgccaga gagctttt
//
LOCUS       DATBTE010000179         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1389088, whole genome shotgun sequence.
ACCESSION   DATBTE010000179 DATBTE010000000
VERSION     DATBTE010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389088"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..>1639
                     /gene="pglX"
                     /locus_tag="VIG33_06610"
     CDS             61..>1639
                     /gene="pglX"
                     /locus_tag="VIG33_06610"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF033452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HEY8270543.1"
                     /translation="MFLSSLQSLRERILRQNTLLSMAHLGANAFDSISGEIVSTTAFV
                     LENANQPTYQGNYLRLIDGSSESEKENAIHEALKNPACGWNYRASAADFNKIPGSPIA
                     YWLSNGALKAFEEGTPFENAAPTRKGMVTARNAVYVRDWHEVSINRSGLDKFASREEA
                     KDSGKKWFSYLKGGGFRRWYGNKFNLVNWENDGYLLQNTRHPAEDRVWATNFNLDFIF
                     NENVNWGAVASTGFSARISKRGELFDAGGSACFPTKVNTKSILAFLNSKVTGILLEAL
                     NPTLNFQAGNISDLPLTSALNNKSIIENSHAAIEQTKSDWNSYETSWDFTTLPLLNYE
                     YFQPTLKTTYQKLRSRWLEMTLEMQRLEEENNRIFIEAYGLQNELTPEVRLGEITLTC
                     NPHYRYGGDKSEENLEALLLADTMRELISYAVGCMFGRYALDKPGLILANQGETIEDY
                     LRQVPEPSFPVDDDNVIPMLDGDWFTDDIAERFRKFLRVTFGEEHYEENLNFVQKALG
                     KNGKARDIRDYFLKDFYN"
BASE COUNT          465 a          382 c          399 g          393 t
ORIGIN      
        1 cgcaatctcg accttgctca gaaatatgga gtggtaacaa tggttaccat gcagagttgg
       61 atgtttttat cttcgctcca gtcgcttcga gaaagaattc tcagacaaaa cacactgctc
      121 tcaatggcac atcttggagc gaacgccttt gacagcatta gtggagagat tgtatcaacc
      181 acagcctttg ttctggagaa cgcaaatcaa ccaacatatc aaggcaacta cttgcgctta
      241 atcgatggta gttctgaaag cgaaaaagaa aacgcaattc atgaagctct taagaaccct
      301 gcctgtggat ggaactatcg tgcctcagca gctgatttta ataagattcc cgggagtcca
      361 attgcatatt ggttgagcaa tggtgcatta aaggcatttg aggaggggac gccatttgaa
      421 aatgccgcac ctactcggaa aggcatggtt accgcccgaa acgctgtcta tgttcgtgac
      481 tggcacgagg tgtcaatcaa ccgctccggc ctggacaaat ttgcatcaag agaagaagcg
      541 aaggattctg gcaagaagtg gttttcgtat cttaaaggcg gcggttttcg tcgttggtat
      601 ggaaataagt ttaatctcgt aaactgggaa aatgatggtt atctgttgca gaatactcga
      661 catcctgctg aagaccgagt ttgggcaacc aacttcaacc tcgatttcat ctttaacgaa
      721 aatgtcaact ggggcgcagt tgcttcgact ggtttttctg ctcggataag taagagagga
      781 gagctatttg atgcaggagg atctgcatgt ttcccgacaa aagtaaatac taaaagtatt
      841 ctggcatttc taaattcaaa agtgacagga atcctccttg aggccttaaa tccaacactt
      901 aactttcagg cgggaaatat ctcggacttg cccctaacgt cggcactgaa caacaaaagc
      961 atcatagaga acagtcacgc tgcaatcgaa caaacaaaaa gcgactggaa ctcctacgaa
     1021 acctcttggg acttcaccac tctgccacta ctgaattatg aatattttca gccaacacta
     1081 aaaaccacct accagaagct ccgcagtcgc tggctagaga tgacgctgga aatgcagcgt
     1141 ttagaagaag agaacaaccg tatctttatc gaagcatacg gtctgcagaa tgagctgaca
     1201 cctgaggtgc ggctgggcga aatcacgctg acctgcaacc cgcactatcg ttacggcgga
     1261 gacaagagcg aggagaatct ggaggcgctg ttactggccg acactatgcg cgagctgatc
     1321 tcctatgccg tgggctgtat gttcggccgt tacgcactgg acaagccggg cttgatcctg
     1381 gctaaccagg gcgagaccat cgaggactac ttgagacagg ttccggagcc tagctttcct
     1441 gtggacgatg ataacgttat ccctatgctt gatggagatt ggttcaccga cgacatcgcc
     1501 gagcgcttcc gcaagttcct gcgcgtcacc tttggcgagg aacattacga ggaaaatctc
     1561 aattttgttc aaaaagcgtt aggcaagaat ggcaaggctc gcgacatccg cgattacttc
     1621 ctcaaggatt tctacaacg
//
LOCUS       DATBTE010000180         7299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1797922, whole genome shotgun sequence.
ACCESSION   DATBTE010000180 DATBTE010000000
VERSION     DATBTE010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7299
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797922"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2555)
                     /gene="rpoC"
                     /locus_tag="VIG33_06615"
     CDS             complement(<1..2555)
                     /gene="rpoC"
                     /locus_tag="VIG33_06615"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY8270544.1"
                     /translation="MNFFDKPKDPLSFDAVRVSLASPEMIRDWSFGEVKKPETINYRT
                     FKPERDGLFCAKIFGPIKDYECLCGKYKRMKYRGVICEKCGVEVTQTKVRRERLGHIE
                     LATPVAHIWFLRSLPSRIGNLLNLSLKDVEKVLYCEAYVVIDPMETTLEESSVLSEEA
                     YQGALNEFGPVFKAGMGGEAVRELLRKIDPEYLSRKLRMEIKETKSEAQIKKLSKRLK
                     VVEAFKNSINKTEWMMLEALPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLK
                     RLQELNAPDIIIRNEKRMLQEAVDALLDNGRRGKTFTGPNKRPLRSLSDMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPTLKLHQCGLPKAMALELFKPFVYNKLEEKGFATTIKQ
                     AKKIVDQQAVEVWDILSDVVKEHPVLLNRAPTLHRLGIQAFEPVLHEGKAIQLHPLVC
                     TAFNADFDGDQMAVHVPLSVEAQVEARVLMMSTNNILSPASGKPIINPSQDIILGMYW
                     LTRSRPGAKGTGKIFSSVTEAQYAYETGLVDLQSTCKVRINGKIEETTVGRAILSDSV
                     PREVPFNEVNLVMNKKQIAALIDKTFRLAGAKATCILADKIMELGFKYSTAAGMSICI
                     DDMVIPASKATLIADAEKQVQEIQQQYDEGLITDGERYNKVVDIWAQTADRVAKEGMS
                     AIEKQKFVIDGKETVGPSFNPIFIMADSGARGSANQIRQLGGMRGLMAKPSGEIIETP
                     ITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDVVVSETDCGT
                     TDGLEVTPLYEAGEIIQPIGERLLGRTALKDVLDPYTNNVVIPANHEFTENDV"
     gene            complement(2609..6817)
                     /gene="rpoB"
                     /locus_tag="VIG33_06620"
     CDS             complement(2609..6817)
                     /gene="rpoB"
                     /locus_tag="VIG33_06620"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEY8270545.1"
                     /translation="MDKTPITASNLRIRKSFAQNKQVIDIPNLIELQKSSYEAFLQKD
                     TDPDRRGEDGLNGVFKSVFPISDFNNTASLEFVSYTLEPAKYDVDECRQRGMTFAAPI
                     KVTLRLIVFDVDEETEARSIRDVKEQEVYLGEIPLMTANGSFIINGTERVVVSQLHRS
                     PGVFFDHDGGKNATSGKLIYSARVIPYRGSWLDAEFDQKDLIHVRIDRRRKFPVTILL
                     KALGFNTEKLLEFFYDLDEVYVREGKLFRKLDIERMSGQRAIADIVDPKTQEVLVKAG
                     RRITRAIVKKIKDINLTEIEVDPEDLDGKVLAKPLIDESTGEIIADANAELSVSLIKK
                     AIALGIDHFFMIFFDGLTVGPYLRNTLLVDKVNNKDEALIEIYKRLRPGEPPTLEAAT
                     TFFHRLFFDPETYDLSEVGRIKINHKFGISMEECPPEFRTLTHKDILSTVKTLIDLKN
                     GRGIIDDIDHLGNRRVRSVGELLENQYRIGLVRMERAIRERMSLQDVETMMPHDLVNA
                     KPVNAVVKEFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRDRAGFEVRDVHPT
                     HYGRICPIETPEGPNIGLISSLATYARINSYGFIETPYRKVDAGSISSDITYMSALEE
                     AGHYIAQAPRDEKGHRNIQTPSTTTRRDGEYEIVDKDKVTLMDVSPSQLVSIAASLIP
                     FLEHDDANRALMGSNMQRQAVPLLRSRAPLVGTGVERLVARDSGTSVVALNDGIVEEV
                     DASRIVIRRFAKGGELGANVDIYNLTKYQRTNTNTCFNQKPIVKVGDRISKSDIVADG
                     PATELGELALGQNILVAFTPWMGYNFEDSILISERLLKEDVYTSIHIEEFECIARDTK
                     LGKEEITRDIANVGEEALKDLDTSGIIRIGAEVRPGYILVGKVTPKGETQLSPEEKLL
                     RAIFGEKAGDVRDTSLRVPSGVYGTVIDAQVYSREGADRDERLSSIIEGKKRKLEKDL
                     QVEQNVIRNNAISKLRDILVGKVTTGVLLNEDGSQKLLNKGQNLTEADLETIPFELLS
                     YIPLDQDLEFQVNKIIDGARNQLDAVKLVFNEKIERLRKGDELPPGVIKMVKVYVAIK
                     RKLQVGDKFAGRHGNKGVVSKVLPLEDMPYLADGTPVDMVLNPLGVPSRMNIGQILEV
                     HLGWAARNLGTQLSAYIQNFQSEEARKALKDTFSDTEISAKIDKADDASLKQMLVKMK
                     DGVHVGTPVFDGAREADVKGLLVKANLPERAQAILFDGRSGEPFTNPVTVGIMYMLKL
                     HHLVEEKIHARSIGPYSLVSQQPLGGKAQFGGQRLGEMEVWAIEAYGAAYSLQEFLTV
                     KSDDVAGRTRMYESIVKGENVLEPGLPESFNVLVKELQSLALNIELMESNILRDQVEG
                     TEDGEVEELVAAVVPKAEEPSNQH"
     gene            complement(7044..>7299)
                     /gene="rplL"
                     /locus_tag="VIG33_06625"
     CDS             complement(7044..>7299)
                     /gene="rplL"
                     /locus_tag="VIG33_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEY8270546.1"
                     /translation="VAVAAGAAAAVVEEKTAFDVILLDAGANKINVIKEVRALTGLGL
                     AEAKALVEAGGKAVKEGATKEDAEKIKKSLEAAGAKVQVK"
BASE COUNT         2180 a         1742 c         1378 g         1999 t
ORIGIN      
        1 ttaacatcat tctcagtgaa ctcatggttc gcaggaatta cgacgttatt agtgtaagga
       61 tccaaaacat ctttcaatgc agttctaccg agaagacgtt caccgatggg ttgaatgatt
      121 tccccagctt cgtaaagtgg agtcacttcc aaaccatcag ttgtaccgca atctgtttca
      181 ctgacaacaa cgtcttgagc cacgtcgacc aaacgacgag tcaagtaacc agagtttgca
      241 gttttcaaag ctgtatcggc caaaccttta cgagctccat gcgtagaaat gaaatactgg
      301 agtacagtca aaccttcacg gaaatttgct gtgatcggag tctcgatgat ctcaccagaa
      361 ggttttgcca ttaagccacg cataccaccc aactgacgaa tctgattcgc ggatccccgg
      421 gcaccggagt cagccatgat gaagatcggg ttaaaagaag ggccgacagt ttctttgcca
      481 tcgataacaa atttttgttt ctcgatcgca ctcatgcctt ctttagcaac acggtctgct
      541 gtttgagccc agatatccac taccttattg tagcgctcac catcggtgat caaaccttca
      601 tcgtactgtt gttggatttc ctgaacttgc ttttcagcgt ccgctatcaa agtagccttg
      661 gaagcaggaa taaccatgtc atcgatgcaa atagacatac ccgcagccgt tgaatattta
      721 aatccaagct ccatgatttt atcagccaaa atacaagtcg cctttgcgcc ggccagacgg
      781 aaagtcttat cgatcaaagc tgcgatttgc ttcttattca tcacaagatt cacttcattg
      841 aaaggaacct cacgaggaac agaatctgaa agaatagcac gacccacggt cgtctcttcg
      901 attttgccgt taatacgcac tttacaagta gattggagat caaccaaacc agtttcataa
      961 gcatattgcg cttcagttac actcgagaag attttgcctg ttccttttgc tccagggcga
     1021 gagcgagtca accaatacat tcccaaaata atatcctgag atggattgat gattggctta
     1081 ccagaagctg gagaaagaat attgttcgtc gacatcatca aaacacgagc ttccacttgt
     1141 gcttctactg acaatggaac gtgaacagcc atttggtcac catcgaaatc ggcattgaac
     1201 gccgtacaaa cgagtggatg caattggatt gccttacctt catggagaac tggctcgaaa
     1261 gcttgaatac caagtctgtg aagagtaggc gcacgattca aaagcacagg atgttcttta
     1321 actacatccg aaagaatgtc ccaaacttca actgcttgct ggtccacgat tttcttagcc
     1381 tgcttgattg tggtcgcaaa acctttttcc tcaagtttat tgtaaacgaa aggtttgaac
     1441 agttccaacg ccatggcttt aggcagacca cactgatgaa gcttcaaggt aggacctact
     1501 acaatcactg aacgaccaga gtagtctacg cgcttaccaa gaagattttg acggaaacgg
     1561 ccttgtttac ctttgagcat atcagaaagg gagcgcaaag gacgtttatt tggcccggta
     1621 aatgtcttcc cacgacgtcc attatccaac aatgcatcca cagcttcttg aagcatgcgt
     1681 ttttcattac gaataatgat gtctggagca ttcaactcct gaagacgctt caaacggttg
     1741 ttgcgattaa tcacacggcg ataaagatca tttagatctg aagtcgcaaa gcgcccacca
     1801 tctaacggaa ccaagggacg aagatcagga ggaagcactg gaagggcctc taacatcatc
     1861 cactcagttt tattgataga atttttgaaa gcttctacaa ctttcaagcg tttagacaac
     1921 tttttaattt gagcttctga cttcgtctct ttaatctcca tacggagctt acgagagagg
     1981 tactcaggat cgatttttct caaaagctca cgaacagctt cgccacccat tcctgcttta
     2041 aaaacaggac cgaactcatt aagagcccct tggtaagctt cctcagaaag aaccgaactc
     2101 tcctcgagag tggtctccat tggatcgatc actacatagg cttcgcaata aagaaccttt
     2161 tcaacgtctt tcaatgaaag gttgagtaag ttcccgatac gagaaggaag tgagcgcaag
     2221 aaccaaatat gagccacagg agttgccaat tcgatatggc caagtctctc acgacgcacc
     2281 ttggtttgcg tgacttcaac gccacacttt tcgcagatca ctccgcggta tttcatacgt
     2341 ttgtacttac cgcacaagca ttcgtaatcc ttgataggac cgaagatttt tgcgcagaac
     2401 aaaccatcac gttctggttt gaatgtacga tagttgattg tctctggttt tttcacttca
     2461 ccaaatgacc agtcacggat catttcaggc gacgccagag aaactctgac ggcgtcaaaa
     2521 ctaagtggat cttttggttt gtcaaaaaag ttcaacaagt ctctcaaggt tcacccctac
     2581 ttattcttgt tgttaattaa attaattctt agtgttgatt gcttggctct tccgctttcg
     2641 gaacaaccgc cgcaaccaac tcctctactt caccgtcttc agtcccttca acttgatctc
     2701 tcaagatatt ggactccatc aactcaatat ttagcgcaag agattgaagc tctttcacca
     2761 acacattgaa tgactcaggt aatcctggct caaggacatt ttcacctttc acgatgcttt
     2821 catacatgcg ggttcttcct gccacgtcat cagacttaac agtaaggaac tcttgcaacg
     2881 aataagcggc accgtatgcc tcaatcgccc aaacctccat ctctccaaga cgttgaccac
     2941 caaactgagc tttaccaccc aaaggttgtt gtgaaacaag agagtacggt ccaatagaac
     3001 gcgcatggat cttttcttcg accaaatgat gcaatttcaa catatacatg atgcctacag
     3061 tgacaggatt ggtgaacggc tcacccgaac gaccgtcaaa taggattgct tgagcacgct
     3121 caggaagatt tgcttttaca agcaagcctt taacatctgc ttcacgagca ccatcaaata
     3181 ctggcgtccc tacatgtacg ccatctttca ttttcacgag catttgcttc aatgaggcat
     3241 catccgcttt gtcaattttc gcagaaattt cggtatcgct aaacgtatct ttcaatgcct
     3301 ttcgcgcttc ttcagactga aagttttgaa tatacgctga aagttgcgtt cctaaattac
     3361 gagctgccca acccaagtga acttctaaaa tttgcccaat gttcatacga gaaggaacac
     3421 ccaatggatt tagaaccatg tctacaggcg taccatctgc caaatatggc atatcttcca
     3481 aaggcaacac tttagaaaca acccctttat taccatggcg accggcgaat ttatcaccta
     3541 cttgaagttt acgtttaata gcgacgtaaa ctttaaccat tttaataacc ccaggaggca
     3601 gttcatcacc cttgcgaaga cgctcaatct tctcattgaa gacaagtttt acagcatcta
     3661 gctgattacg agcgccatcg ataattttat ttacctggaa ctcaagatct tgatcgagag
     3721 gaatataact caaaagttca aaaggaattg tttcgaggtc agcctcagta aggttttggc
     3781 ctttattcaa taacttttga cttccatctt cattcaaaag aacgcctgta gttactttac
     3841 ctacaaggat atctcgaagc ttggaaatag cgttatttct aataacgttc tgctcaactt
     3901 gtaaatcttt ttcaagttta cgttttttac cctcgataat tgaagacaaa cgctcatcac
     3961 gatctgcacc ttcacgagaa taaacttgag catcaatcac tgtaccataa acaccagatg
     4021 gaacgcgaag agatgtatca cgaacatcgc ccgccttttc gccaaagatt gctctcaata
     4081 gcttctcttc aggagaaagc tgggtttcac ctttaggcgt cactttaccc acaagtatat
     4141 aaccagggcg aacttcagcg ccgatacgga taataccaga agtatccaaa tccttaagcg
     4201 cttcttcacc tacattcgct atatcccgag ttatctcttc tttacctaat ttggtatcac
     4261 gagctataca ttcgaattct tcaatatgaa tagaagtgta aacatcctct tttaaaagac
     4321 gctcagaaat cagaatagaa tcctcgaagt tataacccat ccaaggagtg aacgcgacca
     4381 gaatattttg cccaagagcc aactcaccga gttccgttgc cggaccatca gctacgatat
     4441 cgctcttaga aatacgatca ccaaccttaa cgataggctt ttgattgaaa catgtattcg
     4501 tgtttgttct ttgatactta gttaaattgt aaatatcaac attcgcacct agctctccgc
     4561 cttttgcaaa acgacgaatt acgatacgag aagcatcaac ttcttcgaca ataccatcat
     4621 taagcgccac tacggaagtt ccggagtcac gagcaaccag cctttcaacg cctgttccaa
     4681 caagtggcgc tcgagagcgc agcagaggca cagcctgacg ctgcatattc gatcccatca
     4741 atgcccgatt ggcatcatca tgctcgagga atggaatcaa ggatgcagca atagaaacca
     4801 actgagaagg agaaacgtcc atcaaagtta ctttgtcttt atcgacaatc tcatattcac
     4861 catcacgacg cgtagtcgta gaaggagttt gaatatttct atgccctttt tcatcgcgtg
     4921 gagcctgagc aatataatga ccggcctctt ccaatgctga catataggta atgtcagaag
     4981 agatactacc agcgtcaact ttgcgatatg gagtttcaat aaaaccatag ctgttaatac
     5041 gagcataagt tgccagagaa gaaatcaaac caatgtttgg tccctctgga gtttcaattg
     5101 gacaaatacg accataatgc gttggatgga cgtcacgaac ctcaaatcct gcacgatcac
     5161 gagttaagcc gccgggcccg agcgctgaca aacgacgctt atgagtaatc tcggaaaggg
     5221 ggttcgtttg gtccataaac tgtgacaact gagaagagcc aaaaaactcc ttaacaactg
     5281 catttacagg cttagcattt acaagatcat gaggcatcat tgtctcgacg tcctgaagag
     5341 acatacgctc acgaatagca cgctccatac gaacgagacc aatacgatat tgattttcga
     5401 gcaattcacc cacagaacga actcggcgat tccccaagtg atcaatatca tcaataatac
     5461 cgcgaccatt tttaaggtcg ataagagtct taactgtaga aagaatatcc ttatgcgtca
     5521 gggttctaaa ctctggcgga cattcttcca tagaaatgcc gaatttatga ttaatcttaa
     5581 tacgacctac ttctgagagg tcataagttt caggatcgaa aaataaccga tggaagaagg
     5641 tagttgccgc ctctagagtt ggaggctccc caggacgaag acgcttataa atttcaatca
     5701 aagcttcgtc tttgttattt actttatcta caagcaaagt atttctaaga taaggaccta
     5761 cagtcaatcc atcaaagaag atcataaaga agtgatcaat acccaatgca attgcttttt
     5821 taataagact aacactcaat tcagcattag cgtcagcaat gatctcaccc gtggactcat
     5881 caattaaagg cttagcaagg accttcccat ccaaatcctc tgggtccacc tcaatctcag
     5941 tcaaattaat atctttaatt tttttaacga tagcccgggt aattcgacga cccgctttta
     6001 caagaacttc ctgggtctta gggtcgacaa tgtcagcaat ggctctttgt ccagacattc
     6061 tttcgatgtc caattttcgg aaaagttttc cttctctaac gtaaacttca tcgagatcat
     6121 aaaaaaactc taaaagcttc tcagtattaa aaccaagcgc tttcaagagg attgtaactg
     6181 ggaattttct tcttctatca atacgaacgt ggatcaagtc tttttggtca aattcagcat
     6241 ctaaccatga accacgataa ggaataacac gagcagaata gataagcttt ccggaagttg
     6301 cgtttttacc accatcatgg tcaaagaaaa cgccgggaga acgatgcaac tgagaaacaa
     6361 caacgcgctc agtaccatta ataataaaag agccattcgc agtcataaga ggaatttcac
     6421 cgagataaac ttcttgttct tttacatctc ggatactgcg tgcttccgtt tcctcgtcaa
     6481 catcaaatac aataagacga agggtgactt taataggcgc tgcaaaagtc atcccacgct
     6541 gacgacattc atcaacgtcg tatttagccg gctcaagagt atagctaaca aactcaagac
     6601 ttgcagtgtt gttaaaatca ctgataggaa aaacggattt aaaaacaccg ttaagaccat
     6661 cttctccacg acggtctgga tctgtatctt tttgcaaaaa agcttcgtaa gaactctttt
     6721 gcaattcgat caaattcgga atgtctatga cttgtttgtt ttgtgcgaaa gattttctga
     6781 tacgcagatt tgaggccgtg attggagttt tatccatcga atctccctta aagagtcaaa
     6841 ccatctaggc aactgaaatt attttcttat ttcagtacca aaaacacatt tgaactgaac
     6901 aaaatcagtt cgttcggcgt aaagcttagc cgagaaaata taatacccct tagcgcaggg
     6961 gtcaacgtcg catttcaata aaaatgcaaa atggcctctt ttttgcattc aaaataaaaa
     7021 gaggccataa actcttaaaa tgactactta acctgaacct ttgcgccagc tgcttcaaga
     7081 gactttttga ttttctcagc gtcttcttta gtagcccctt ctttaacggc tttgccacca
     7141 gcttcaacaa gtgctttcgc ttcggcaaga ccgagacctg ttaaggcacg aacttcttta
     7201 attacgttaa ttttatttgc acctgcgtca agaagaataa cgtcaaaagc agttttttct
     7261 tctactacag cagcagccgc acccgcagct acagctaca
//
LOCUS       DATBTE010000181         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_433190, whole genome shotgun sequence.
ACCESSION   DATBTE010000181 DATBTE010000000
VERSION     DATBTE010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433190"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..>1621
                     /locus_tag="VIG33_06630"
     CDS             46..>1621
                     /locus_tag="VIG33_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270547.1"
                     /translation="MVSETNGISLKDTLNLLISSELNAESQEGLKLVVEFRELIDQFV
                     TNKVTINRLLIHPLSECLFELFKNFPIKYNEEHIHLTGSITAEFIYPRLKKLLEGSHK
                     ALYEQKIKEVYGPNSLPIRSVADVDKLIRLQEAEGFLTYLKILYLPKLVLINKAVHAE
                     AAYDMANELYTKFNVGRIRLKFTLDRATSNSSEQIPGIDEVTPEDVVLGLYEGYKKFQ
                     DEHPDFDFVLSPCFRKEPNFFDATKYKSRKEHFMDQINEMIRMIDKYPFLQKHMTDVD
                     TVGDERELYRKEHFYEMQMGFRKLQYRGFQIRSHHGETWHTLKKGIQAVDNAMNIWHI
                     DTLEHGISLGINPNVYFHRLYQEVSHQNKQGIAVTTKDPHYRELSELDWGNYRPVLDK
                     LLKGEPLGEQENTDFLKAKFHTAREVEHYQHDVLNRMIQKEISLISLPSSNNKLTGKF
                     EDYKDHPFSWWEKKNVQLGVGTDNYVTLNTNFIQELLILLYADPSDLKITKLLMVATK
                     ENRRPYISHLLWKMRKK"
BASE COUNT          508 a          349 c          317 g          447 t
ORIGIN      
        1 ttaaatcccc aaacaaaaaa agaactcgtc aaatggctca ccctgattgt cagtgagacc
       61 aacggtattt cccttaagga cactttgaat cttttgatct cctccgagtt aaatgcagag
      121 agccaggaag gcttgaagct ggtggttgag ttccgtgaat taatcgacca attcgtgaca
      181 aataaagtga ccatcaacag gcttttaatc catcctcttt ctgaatgtct attcgaactc
      241 ttcaaaaatt ttcctattaa atacaacgaa gagcatattc acttaaccgg atctatcacc
      301 gccgagttta tttatcctcg tctaaaaaag ttgctcgaag ggtcgcataa agccctctat
      361 gagcaaaaaa ttaaagaagt ttacggtccc aattcgctgc caatccgttc ggtggctgat
      421 gtcgataaat taattcgttt gcaagaagca gaaggatttc tgacctattt aaaaattcta
      481 tatctaccaa aattggtttt gatcaataaa gcggttcacg ctgaagccgc ttatgatatg
      541 gcgaatgagc tttacactaa attcaacgtg ggacgcattc ggcttaagtt tactttggat
      601 cgtgcaactt cgaactcttc agaacaaatt ccagggattg atgaagtcac ccctgaagat
      661 gtggttttgg ggctttacga aggttataaa aaatttcagg atgaacatcc ggactttgat
      721 tttgttcttt ctccctgctt tcgaaaagaa ccgaactttt tcgatgcgac gaaatacaaa
      781 tcccgcaaag agcatttcat ggatcagatc aacgagatga tccgtatgat cgacaagtat
      841 ccatttttgc aaaaacacat gaccgacgtg gacactgtcg gagatgaacg tgaactctat
      901 cgcaaagagc atttctatga aatgcaaatg ggcttccgta aactgcaata tcgcggtttc
      961 caaatcagat cgcatcacgg cgaaacttgg catactttaa aaaaaggcat tcaggccgtg
     1021 gataatgcca tgaatatctg gcacattgac accctcgagc acggaatcag ccttggcatc
     1081 aatcccaatg tttacttcca tcgcctttat caagaggttt cccatcaaaa caagcaaggc
     1141 atcgctgtaa ctaccaaaga tcctcattat cgcgagcttt cagaattaga ctggggtaat
     1201 tacagacccg tcctggataa gcttttaaag ggagagcctc tcggcgaaca agagaacacc
     1261 gatttcttga aagctaaatt tcataccgcg cgtgaagtag aacattatca acatgacgtt
     1321 ttgaaccgca tgattcaaaa agagatctcc ttgatttctc ttccgtcttc caacaataaa
     1381 ctcacgggaa aatttgaaga ctataaagat catccctttt cttggtggga aaagaaaaat
     1441 gttcaattgg gcgttggaac ggataattac gtcaccttga atacgaactt catccaagaa
     1501 cttttgattc tactttatgc ggatcccagt gatttgaaaa ttaccaagct cttaatggtg
     1561 gcaacgaaag agaatcgtcg tccgtacatc agtcatctgc tttggaaaat gcgtaagaaa
     1621 a
//
LOCUS       DATBTE010000182        20726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1047892, whole genome shotgun sequence.
ACCESSION   DATBTE010000182 DATBTE010000000
VERSION     DATBTE010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 20726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20726
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047892"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VIG33_06635"
     CDS             <1..518
                     /locus_tag="VIG33_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270548.1"
                     /translation="SYGSPNISKSIPLSVTLDSSNKIIACVALAKMTDNIWSRIPGTN
                     WDIQYSGGKIGVGTATPQASLDVAAGSIRPGSLASAGPCGANNEGAIAYDQTTHTPVF
                     CNGTSWSGMFHSFGGMWINYSKWDKGCAGDYSGEAYSVNPFTGTTSCPDGFTDTVLNP
                     MSCSRIHFCWK"
     gene            complement(1001..1360)
                     /locus_tag="VIG33_06640"
     CDS             complement(1001..1360)
                     /locus_tag="VIG33_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8270549.1"
                     /translation="MSLGQLMIFVSSLQKAKSFYVDLIGLEVAHDMSEENGMLIMKND
                     GSYLTIHEGFKQKNTDQDACRITPIFKVKDVQATRKILETNGVHLEGGITETSVHHYQ
                     MLKDFDGNWIEVAQFKN"
     gene            complement(1476..1799)
                     /locus_tag="VIG33_06645"
     CDS             complement(1476..1799)
                     /locus_tag="VIG33_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270550.1"
                     /translation="MNKAEIVIAMYRPHDGKLNELEVLVRKPYSTLKEYGLATTREPF
                     IGRSMDGSILEVFEWVSSEAAEKAHDHPAVAKIWEAMAMVCDFGKLNELPEAKNRFPH
                     FHSAF"
     gene            complement(1796..2107)
                     /locus_tag="VIG33_06650"
     CDS             complement(1796..2107)
                     /locus_tag="VIG33_06650"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8270551.1"
                     /translation="MGKSRAERELEEFDITFSALSHEARRRILVVLLSRGGKMTAGQI
                     VERFSCSWPTMTRHLQVLEDAGLVRIEKIGRERHYILNRQQIVDVVGNWLSWFEKEKT
                     L"
     gene            complement(2290..2442)
                     /locus_tag="VIG33_06655"
     CDS             complement(2290..2442)
                     /locus_tag="VIG33_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270552.1"
                     /translation="MVQKKSAEIYKKNGAIDDWTFGPENLKAKYGCMSFSQEIPVNAG
                     EELFSH"
     gene            complement(2595..3083)
                     /gene="msrB"
                     /locus_tag="VIG33_06660"
     CDS             complement(2595..3083)
                     /gene="msrB"
                     /locus_tag="VIG33_06660"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY8270553.1"
                     /translation="MKEKSQSKNEVACELVCGLPENDAELRKLLTPEQYEIIARNGTE
                     RPFQNAFWNHHEDGLYVDVVSGEPLFASTDKFDSGTGWPSFTRPVKPEVVRDIEDLSH
                     GRIRTEVRSTKSNSHLGHVFDDGPDPTGLRYCINSAALRFIPVSKLKEAGYEDYLNLF
                     EG"
     gene            complement(3115..3615)
                     /gene="purE"
                     /locus_tag="VIG33_06665"
     CDS             complement(3115..3615)
                     /gene="purE"
                     /locus_tag="VIG33_06665"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEY8270554.1"
                     /translation="MSKAKSPAKNAEVMIVMGSLSDYPTMKRAEEIFKEFGVRFHTKI
                     VSAHRTPELMFAEGKAASENYKVVIAGAGGAAHLPGMMASLTTLPVIGVPVPHGALGG
                     QDALYSIVQMPEGIPVATVAIGNSGNAALLAVQILAIQDSELAKRLLKYKENLRNKVA
                     EQNKKI"
     gene            complement(3615..4787)
                     /locus_tag="VIG33_06670"
     CDS             complement(3615..4787)
                     /locus_tag="VIG33_06670"
                     /inference="COORDINATES: protein motif:HMM:NF014298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEY8270555.1"
                     /translation="MSQMKIGILGGGQLGRMLWEASCSLGLPPSPVYHPEVQCPAGRA
                     HANIIQGEITEIPKLSAFFRSVDSYAIENEFLDIKSIEQAWGSSRTQPAPSLEGLRIA
                     QDKLEQKKFFQKNGFPTSEYLEILPADLEAAEKLEQLHKKWQGFVIKKARMGYDGKGN
                     FAAAPSEPLNFSNIKNFCQEAFASQSRVYAEKFVPFVKEVALVSCQSFAGDFGSYPLI
                     ETIQKKGVCFLAMKALHEEKNEARAREMARLIGTKLNLLGTFAVEFFITQSGELLVNE
                     MAPRVHNSGHFTQKAAKSSQFEMHLKSYWQKQFNPQDFETAKAFAMVNFLGPEGVRGP
                     VGRPKNPQIYWYDKEMTSPGRKLGHMIAQSGDEKALPELLEALKAAESQWQESLRP"
     gene            complement(4784..5836)
                     /gene="purM"
                     /locus_tag="VIG33_06675"
     CDS             complement(4784..5836)
                     /gene="purM"
                     /locus_tag="VIG33_06675"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEY8270556.1"
                     /translation="MKSEKGAYEKAGVNRDLADQFVGSISMMVKSTLNANVKSSVGGY
                     ASLYSLNAKQLLAASTDGVGTKLKLAFEWDSHHTVGIDLVAMSVNDLLCVGAKPLFFL
                     DYFATGKLKPKTAKEVLAGIVQGCLQGGCALIGGETAEMPGFYEAGEYDLAGFAVGTV
                     DKKKALPLKNIKPGDVLIGISSSGFHSNGYSLVRKLFAAWKPTKALSKKTLAEELLRP
                     TVIYTKALEKLLANGSVKGLAHITGSGFLNVPRMSEKVSYEIHLPPLNERPKAYQWLE
                     SLKDSSLTFEEKSQTFNMGIGMVIACDPEKAATILAHLKKNNFKSWKLGQVIRKNKQC
                     EVAVTDGSQKALLVYS"
     gene            complement(5853..7412)
                     /gene="purH"
                     /locus_tag="VIG33_06680"
     CDS             complement(5853..7412)
                     /gene="purH"
                     /locus_tag="VIG33_06680"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY8270557.1"
                     /translation="MSVTTAPLKIRRALLSVSDKTDLVDLAQAMHKTGTELYASGGTL
                     KTLQDAKIPCRPAEQLSETPEAFQGRMKTLSFKIFSGILARREDPKDKLDWEKLNIKL
                     LDAVVVNFYPFQKNMDKNLSRYELTELIDIGGPALVRAAAKNAPDVVVLTDPNQYSAV
                     IEQLNSQGAVERKTAIEVAAQAWDLVTDYDLSIQSVFGAGTKVLRYGENPHQKASLKV
                     HANSPLDWNHPLTSTELSYNNIVDLSSAYFVARDLKAAFPDKTSVVIVKHNNPCGVAT
                     VSKSAPKAQLRALEKAWACDPVSAFGGVLIFTDEVDAEILQYFQSRFVELVSAPKLKD
                     TDLKDLLTQRKNLKAVTVKHWDLDSPSMQMTVAGGTLTQSLDQQIEEPLKGVTEVPWQ
                     TQWNALAHFGIFCNKTLKSNSVCLVCEDSEGDYRMVGAGQGQPNRVEAMEKLAIPRAQ
                     SVLNKTDLGECILISDAFFPFRDSVDVAARAGIRTIVQPGGSIKDATVIAACNEHKIA
                     MAFTGYRHFRH"
     gene            complement(7403..8164)
                     /locus_tag="VIG33_06685"
     CDS             complement(7403..8164)
                     /locus_tag="VIG33_06685"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEY8270558.1"
                     /translation="MIKALVLAGDGINCEQEMSHAARAAGFKVDIVHINDLIQKKFSQ
                     DHLKKEYSALFMPGGFSFGDHLGSGRVLALKLTKGLNWNLEDYSANGGIVLGVCNGFQ
                     ALIAMGAFGKKVSITHNIQNKFINRWVELDVELKVAGKKDASANVFLKDLEKMYLPIR
                     HGEGRLLFDSSVDVANCPDFKVSLYYSEDVNGSTQQIAGLTNPSGRVFGLMPHPEAAV
                     RKTQNPQWTLCPEKSHEEEIGMMFFKNAYKEASCL"
     gene            complement(8161..11139)
                     /locus_tag="VIG33_06690"
     CDS             complement(8161..11139)
                     /locus_tag="VIG33_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEY8270559.1"
                     /translation="MFNRTEVILKNELQDPKGMALLARIQKWNPVLSQKIKSLRVVDL
                     FWTKFEGESAEFTEAAKDIWWDEVLQQKHWPESHWVVEREYHPGMTDNLGRTGLEALE
                     ICCRRELLESQVVSGQAYLFVGPELVKEDMEKITSNLISNSLIHRHRVYSRSEFEKYP
                     RFESSEIGPKFILPTGTTPQGQIVPILGMNDDQLIELSKKNLLSLNLQEMRAIQNYFT
                     KEKRNPTDVEVEILAQTWSEHCKHKIFSAEVEYSEEASKKSNIPSKISSLFKTTIAGT
                     TKELPKPWLLSVFKDNAGIVAFSEEEAVCIKVETHNSPSALDPFGGAITGIVGVNRDI
                     LGCGFGAKPIFNTDVFCVGPLELTDGLPSGIIPPAQILEGIRSGVEAGGNQSGIPTVN
                     GALYFDPSFVGKPLVFCGSGGIMPRKIADKLCAEKKIENGDLICMVGGRIGRDGIHGA
                     TFSSLEMTESIPSSVVQLGDPITQRRMTDFLIEARNLGLYRTLTDNGAGGLSSSVGEM
                     ADIAGGARMDVSLAKTKMHGLKPFELVISESQERMTVAVPPKNKAAFLELAHRRGVEV
                     SVLGEFTHSGRFEIFYAESKVADLSLEFLHEGVPPLQLKAHWVEKAAPAGATEPREEK
                     PFQTLKAILQRPNIRSKENLIRQYDHEVQGRSVVKSLQTSKTQRSTPNDAAVLALSTE
                     LKSNNSIGVVVGCGLNPRLSAVDPYIMAQAAVDEAVRNLLCVGAEFSLGDETVLSLID
                     NFCWPDPVEDVEKMAALVRTCYGLREACLALKAPLISGKDSMKNDYKGSQNGKPVKIS
                     VLPTLLVTAMGRMPDYTLARSADFKKAGDKVYKLGPAQLGLAQSEWQGLHAEAKSEVK
                     AVTAPAPNWPKALNLYQWLGSKESSEYLRSAHDLSEGGLLTALAESCFTNNLGLEFSS
                     ALELHLAKTSFGEGFHQFIVSVPAEKAATAEKIWKQKALDFVEVGSLTATAEWKWTFQ
                     DQSHSIAVEELYQAWSRESL"
     gene            complement(11111..11752)
                     /gene="purN"
                     /locus_tag="VIG33_06695"
     CDS             complement(11111..11752)
                     /gene="purN"
                     /locus_tag="VIG33_06695"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEY8270560.1"
                     /translation="MGLNASPTIAILASGRGSNFEAIADAIEAKLLDARIAVVISDRP
                     ESLVLEKAKARGLETLVEKNQKKMKSTLQELNLDYLVLAGFMRILSAEFISAFSDPRG
                     FARIVNIHPSLLPEFPGLESYKKAFEQKLLGKLSRTGITVHFVDAGVDTGLICAQREF
                     SISDCHSVEDVERRGLAVEHELYPQTLQWVLQNQFEIIKKGEKLHVQPHRSHS"
     gene            complement(11743..13086)
                     /gene="purD"
                     /locus_tag="VIG33_06700"
     CDS             complement(11743..13086)
                     /gene="purD"
                     /locus_tag="VIG33_06700"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY8270561.1"
                     /translation="MKILLLGSGGREHAMAWKLLASARVQSLVMAPENSAAVQQLQNQ
                     YANKSVTAWTDSVKGPEALAKLAKKAKEQKVNFVVVGPDNVLADGAVDIFEKEGLEIF
                     GPRQAAARLESSKIFAKKVMQAAKVPTAEGYDVRSVAEAKQLLLDLNWQKKQWVMKAD
                     GLALGKGVEICETQEQALQALERLQVYSKSFVIEERLKGKEISWLAFCDGETCSLLAP
                     VRDYKTLTEDPLSSNTGGMGAVCPVPLEDSTLAAKVRESVFLPTLQEMKKRGTPYKGI
                     LYAGLMIDGEDFWVLEFNARFGDPEAQALLPLMEGDLLEWCEACARSTLHQMPRMVPM
                     SSQVAVYVVAAAPGYPTEPALGQPIRGMDRWLESHHGFFAGVKKQPAAQLAGQPAAQL
                     AGQPAAEWVTSGGRVLGALGLGSSIKEARQKAFAHLKEISFEGMQYREGVGSEWV"
     gene            complement(13083..14081)
                     /locus_tag="VIG33_06705"
     CDS             complement(13083..14081)
                     /locus_tag="VIG33_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY8270562.1"
                     /translation="MLTNRFSKNRLMYEGSVKRIFSSDESANLVFEFTDDYSVFDWGK
                     MPDTIPFKGVGLAKLMSVLFKRLEAQGVKTHYRGFDGERNVLVQKIGVYRPERISENG
                     RNYYTYPKTMEKPFLIPLEVVFRFEVCAGSSFIKRHAEKNYKVGDQFPKPFIEMFTKL
                     EDKDRPLSQEEALKISGLTPAQFVAVSAKTAEVAQTLKTWFGDKGIRLMDGKLEWGLS
                     MEGEIILVDAIGPDEMRLEKQGMQLSKEVLRDFYRKTPWYEELEKAKEEGEAKGYADW
                     KALVKPPPALPPVLKNLVSEMYQALVNEIAAEKLFPCPSLDHVVTELATYLKGASE"
     gene            complement(14068..15531)
                     /gene="purF"
                     /locus_tag="VIG33_06710"
     CDS             complement(14068..15531)
                     /gene="purF"
                     /locus_tag="VIG33_06710"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY8270563.1"
                     /translation="MCGVIGLIGTSQASSRALRGLSLLQHRGQDAAGILSYDDSGFHY
                     VRNLGLVEKVFTEENMRPLTGTVAIGHVRYGTAGKSELRNVQPFLLNYPYGIGLVHNG
                     NLVNFRALTQELRTKYQRQPLADSDTENIINLFAEGLAKNVHKRTPSFEDFCSSVSFI
                     FEKAIGSYSIVSMVAGFGLVAFRDPHGLRPLVMGTRQEDDGKLSYALASESTALSFLG
                     YENFEDIRPGEIVAISMDGGMQRKQLYAQAHRPCMFEWVYFASPTSEIEGSPVYKARI
                     ELGKKMAISVRERMLKDNIGFDIVVPVPETARIAAIALSEELKVPYREVLIKNRYINR
                     TFILDSQDKREQAMHLKLAPVYSEIKDKRVLLVDDSIVRGTTSRKLIELVRMAGAKEV
                     YFASTCPPIKHPCFYGIDFPMEKELLANNKTLQSVEEELGADGVIYQEMEALKEAIFP
                     ENNITPCMACLNKQYPTGIEGVKELIQLRNEDRSAYK"
     gene            15924..16115
                     /locus_tag="VIG33_06715"
     CDS             15924..16115
                     /locus_tag="VIG33_06715"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEY8270564.1"
                     /translation="MRTTLDLNEDLVNQARKLTNIKEKTALIHEALRVLIAQAAQKEL
                     ALMGGTQKQVKPIPRRKAG"
     gene            16119..16475
                     /locus_tag="VIG33_06720"
     CDS             16119..16475
                     /locus_tag="VIG33_06720"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY8270565.1"
                     /translation="MILVDTNVWIEHFKVSSPQLIELLMEGRVVTHEFILGELSLTHF
                     NKKDREKIFERLRALERVKTSKHEEVYDFSFHSKLSEKGIGWIDSHLLHACVTQKLEL
                     LTFDKNLSQLAKKIIR"
     gene            complement(16495..19122)
                     /gene="uvrA"
                     /locus_tag="VIG33_06725"
     CDS             complement(16495..19122)
                     /gene="uvrA"
                     /locus_tag="VIG33_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY8270566.1"
                     /translation="MASNQEFDGIVVKGAKEHNLKDVSVVIPRNKITVFTGLSGSGKS
                     SLAFDTVYAEGQRRYVESLSAYARNFLEQLKKPEVDSVTGLSPAIAIDQKSVSTNPRS
                     TVGTVTEIYDYMRLLFAKVGVPECPTHHLPVSSQTPQQILEEILKKGSKNPQAAKFHI
                     LAPMAQGKKGEFMAEFQRWARKGFVKAQIDGKWIDLEKATKLAKTKVHDIDLVVDQVI
                     LKDSVNRLRLAESINTALSMANGRLVTQTLNGERVSYSLHSSCPQCGYSFPEIEPRIF
                     SFNNPRGACLTCHGLGTADLVEEEEFSEGVVGARRLEKVKYTYKGKKVASSNDEEEES
                     EEMVLSVCPDCQGARLKKESLNVRLQDKNIAELSDLSAVELRDWLAKVSWKSKDQMIA
                     EKISKQILNRLDYLIRVGTGYLSLSRPSRTLSGGEAQRIRLATQVGSSLIGVLYVMDE
                     PSIGLHPRDHHRLLHIIAELKDRGNTILLVEHDEDTIRFADHVVDLGPRAGRLGGEMM
                     AAGTPQEIEANPKSLTGRYLSGELRIPIPRQRRQGNGQKLRLIGASGNNLKNVTLEIP
                     LGTFTAVTGVSGSGKSTLIIDTLFKILAQRFYKAGASPSSFQKLEGIEHIDKVIDINQ
                     RPIGRTPRSTPATYVGLFPMIRDLFAGLPDSKLRGYPPGRFSFNVKGGRCETCLGHGQ
                     IRVEMHFMSDVFVKCETCLGARYNRETLNVKYKGKSISDILNMSVLEALELFKNHPQI
                     YRKLETLNRVGLDYMTLGQSSTTLSGGEAQRVKLSKELSRRGTGKTLYILDEPTTGLH
                     FEDIKKLVELIQDLSHQGNTVLVIEHNLEVVKCADHVIDLGPDGGKAGGLIIATGTPE
                     QIVKVKNSETGKFLKAVLK"
     gene            complement(19192..19683)
                     /locus_tag="VIG33_06730"
     CDS             complement(19192..19683)
                     /locus_tag="VIG33_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEY8270567.1"
                     /translation="MSKDTVPLLPRQHDEDIIVYGDEVGKSELKKIIRALNLKVTTQR
                     MAILKALHEGHRHITAQELFERVNAAHPEIGFATVYRFLKTLSEGHFVAEVRMGGLPA
                     RYELNLKNHHDHLTCVRCGKICEFENKTIESLQEKVAKQFGYQLTHHVLELYGVCPGC
                     QMK"
     gene            19891..20649
                     /locus_tag="VIG33_06735"
     CDS             19891..20649
                     /locus_tag="VIG33_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270568.1"
                     /translation="MIKLISLFLLVLSFAYTSLADPDVPLPGPGAPPSEPPAPQPTPP
                     PSPTPPSPTPPPPDSPSPKPTPKPRQSPRKPPRRTPKVPAPTPTPDPDQDLTPTPTPW
                     PSETPTPSPIPPQESKKTYTVGVGQVARFKDRVFNFFIPAHRSSIRALRITCTRQEIK
                     IKNVTVQFTNGQRSDQFFLVGPLNFGESRVTYMPGEAVFKITVVASSSSIFKGKGAFR
                     LDATAILLRRRNEGKEEPHQIESPLLDENKETPY"
BASE COUNT         5488 a         5365 c         4314 g         5559 t
ORIGIN      
        1 aaagctatgg ctctccaaac atctcaaaat cgatccccct ttccgtcact ctcgattctt
       61 ccaataaaat tatcgcctgt gtggcccttg caaaaatgac tgataacatc tggtcacgca
      121 ttcctggaac caactgggac attcagtaca gtggaggtaa aattggagtg ggaacagcaa
      181 ccccccaagc gagcctggat gtagccgcag gctccattcg acccggcagc ttagcttctg
      241 ctggaccttg tggagctaac aatgaggggg ccattgctta tgatcaaacc actcacactc
      301 cagtgttttg caatggcact agctggtcag gcatgtttca ttcctttgga ggcatgtgga
      361 tcaactattc taagtgggac aaaggctgtg ccggcgatta ctccggtgaa gcctactctg
      421 taaatccctt caccgggacg acaagttgcc ctgatggatt taccgacacc gtgttaaatc
      481 ccatgagctg ttcacgcatc catttctgtt ggaaataatc ttattccgcc atcagacatg
      541 gcagaataga cgaaagctcc ataagaggtc taataagagg tctaggatga tcgaaagaat
      601 tcaacatctt ccaacaaaaa gttttcttct ctttggccct cgcggggtgg ctgctcattt
      661 aattttaggt aaatcgaaaa caaatccctt cgaatagccg ggcaatcggc tcggaactgg
      721 ctttgtaacc cctcaaaggt tcttgcccta aaagacgcct tcaaatgacc accgctgacc
      781 agagagcagt ggttctaaaa cggtttaagt tggattagat tgccactcgg tgttttcttg
      841 aacgtttgca tgaaaaacat cccaaatcat gatttctgtt ttgccaattt cattcatggg
      901 ctttccagat cagagcatag actacgggac gtgtattctc cggctgctcg aaactgtttt
      961 catttcctgt cttagattca gaactcatat tcccatctag ttaattttta aactgagcaa
     1021 cctctatcca gttgccatca aagtccttta acatctgata gtgatggacc gatgtctcag
     1081 tgatgccacc ttctaaatgg acaccatttg tttctaatat ttttctggtg gcttgtacgt
     1141 ctttcacctt gaaaatagga gtgattcggc aggcatcttg gtcggtattt ttctgtttaa
     1201 aaccttcgtg aattgttagg tatgagccat catttttcat gattagcatc ccattttctt
     1261 cagacatgtc atgagccacc tcaagtccaa tcaaatcaac atagaaagac tttgccttct
     1321 gaagtgagct tacgaagatc atcaattgtc caagactcat tttatcctcc ggtttttagg
     1381 ccaagtatca cgctgcaatt gggctgtcta gtagtgaatc gtttggattt ataatgtcca
     1441 ccatgtttga gctggatttt gagcggataa gtcctctaaa acgcgctatg aaagtgcgga
     1501 aatctattct tggcctctgg aagctcattc agttttccaa aatcacatac catcgccatt
     1561 gcctcccata ttttagcaac agctgggtga tcatgagctt tttctgcagc ctcacttgaa
     1621 acccactcaa aaacttcaag aatgctacca tccatcgatc tgccaatgaa gggctctctg
     1681 gtagttgcta agccatactc ttttaatgtc gaatagggtt tgcgtactaa gacttcaagt
     1741 tcgttaagtt taccatcatg aggtctatac attgcgatga caatttctgc cttattcata
     1801 aagttttctc cttttcaaac caactaagcc aatttcctac gacatccacg atttgttggc
     1861 gatttaaaat atagtgtctt tctcgtccaa ttttttctat ccgaacaagt ccggcgtctt
     1921 caagaacttg aagatgccgg gtcattgtcg gccagctaca gctaaagcgt tctacaattt
     1981 gaccagcagt cattttgccg cccctggata aaagaactac aagaatcctt ctccgcgctt
     2041 catgcgacag cgctgaaaac gtaatgtcaa attcttccaa ctctctttca gctctacttt
     2101 ttcccacgca aatatattta gcatatatgc taaatattag tcaattggaa atttgtgtca
     2161 ttcgcgcaat gagttaaaat gaaagattct gaagcgtctt aaactaagcg attgaactcg
     2221 ccgcggacaa ctcgtgataa atcaattaaa ctgcaaactt gatcataaag tttttcgatt
     2281 taaatagact cagtgagaaa aaagttcttc gcctgcattt actggaattt cttgtgaaaa
     2341 agacatacat ccgtactttg ctttgagatt ttcaggccca aacgtccaat catcaattgc
     2401 gccgtttttc ttgtaaattt cagcagattt tttctgaacc atgagaaagt cttcaatatt
     2461 cttttttgga acacggtaaa aataaagtga agtgtgcata tttcgctagt atcacttcac
     2521 cacttaactg tctataaaag gaatgtttcc aaggcgacaa ctcatcagca tcatgacgag
     2581 tacatgatca agctctaacc ctcgaacaaa ttaagataat cctcataccc cgcctctttc
     2641 agcttactca ccggaataaa tctgagagca gcggagttga tacaataacg gagccctgtc
     2701 gggtccgggc catcgtcgaa gacgtgtcct aagtgagagt tggatttggt agagcgcacc
     2761 tctgtgcgaa tcctcccgtg acttaaatct tcgatgtcac gcaccacttc cggttttaca
     2821 ggacgagtga agctcggcca gccagtgccc gaatcaaatt tatccgtcga ggcaaaaaga
     2881 ggctccccgg aaaccacatc cacataaagg ccgtcttcat gatggttcca aaaggcattt
     2941 tgaaaagggc gctccgtgcc gttgcgagcg atgatctcgt actgctctgg agtgagcaat
     3001 ttacgaagtt ccgcatcgtt ctctggaaga ccacagacta attcgcaagc gacctcattt
     3061 ttggattggc ttttttcttt cacgatccac ctcaggctta ttgagtgctt cagattagat
     3121 ctttttattt tgctcagcta ctttgtttct cagattttct ttgtatttta gcaatctttt
     3181 tgccaactca gaatcctgga tcgctaaaat ttgaactgct aaaagagcgg cgttcccaga
     3241 attgccaatg gccaccgtgg ccaccggaat cccctcgggc atttgcacga tggagtataa
     3301 agcatcctga ccacccaagg ccccgtgcgg aaccggaacc ccaatcacgg gcaaggtagt
     3361 gagtgaggcc atcattcctg ggagatgggc ggctcctccg gctccggcaa tcaccacttt
     3421 atagttttca ctggcggctt tgccttcagc aaacatcaac tctggagtgc gatgagcgga
     3481 aacaattttg gtgtgaaaac ggacgccgaa ttctttaaaa atttcttcag cccgcttcat
     3541 ggtaggataa tcggagagac ttcccatcac gatcatcact tctgcattct tagctgggct
     3601 tttagcttta ctcattaggg tctcaaactt tcttgccact gactctcagc agctttcagg
     3661 gcctcgagga gttcaggcaa ggctttttca tctccgcttt gagcaatcat atgcccgagt
     3721 tttcttccgg gtgaagtcat ttctttatcg taccaataaa tctgtgggtt tttgggccga
     3781 cccacaggtc ctcgaacgcc ttcgggacct aagaagttta ccatggcgaa ggccttggcg
     3841 gtttcaaagt cttgaggatt aaactgcttt tgccaataag attttaaatg catttcaaat
     3901 tggctcgatt tcgcggcttt ttgcgtaaaa tgacccgaat tatgaactcg cggtgccatt
     3961 tcattaacca ggagttcccc gctttgcgtg ataaaaaatt ccacggcaaa ggttcccaaa
     4021 agattcaact tggtgccaat caagcgagcc atttcccgag cacgggcttc gtttttttcc
     4081 tcgtgcagcg ccttcatcgc taaaaaacaa acgccctttt tttgaatcgt ttcaatcaaa
     4141 ggataggaac caaaatcccc ggcaaaactt tgacaggaaa ccaaggccac ctcttttaca
     4201 aaaggcacaa acttttccgc atagactcga gactggctgg cgaaagcttc ttggcaaaag
     4261 ttcttaatat ttgaaaaatt caaaggctct gagggagcgg ccgcgaaatt ccctttccca
     4321 tcataaccca tgcgagcctt tttaatcaca aagccttgcc actttttgtg caactgctca
     4381 agcttttcag cggcttctag gtcggctggg agaatttcca aatactccga cgtcggaaaa
     4441 ccattttttt gaaaaaactt tttttgctcc aatttgtcct gagcaatacg cagaccttcc
     4501 aaagacggag ccggctgggt ccgcgaagat ccccaggctt gctcaatgga ttttatatct
     4561 aaaaattcat tctcgatcgc atagctatcc acgctcctga aaaaagcgct gagcttggga
     4621 atctccgtaa tctcaccttg tatgatgttg gcgtgagctc ttcccgccgg gcactgaact
     4681 tcaggatgat agacaggcga tggcggcagg ccaagactgc aactggcctc ccaaagcatg
     4741 cgacctaatt gtcctccgcc taagattcca atcttcattt gactcatgag taaaccagaa
     4801 gcgccttttg cgaaccatca gtcaccgcga cttcacactg tttattttta cgtatgactt
     4861 gtccgagttt ccaggattta aagttatttt tctttaagtg agccaaaatg gtcgccgcct
     4921 tttcaggatc acaggcaatc accatcccaa tgcccatatt aaaagtctga gatttttctt
     4981 cgaaggtcaa agaggaatcc ttcaaagact ccaaccactg ataggctttt ggtcgttcgt
     5041 tcaaaggggg caaatgaatt tcatagctca ctttttcaga catgcgaggc acattcaaaa
     5101 agcccgaacc cgtgatatgc gccaaacctt tgacgcttcc gttggcgaga agtttttcta
     5161 aagccttcgt gtaaatcact gttggtcgca gcagctcttc agcgagggtt ttcttgctca
     5221 aggcttttgt cggtttccac gccgcgaata acttacggac caagctgtaa ccattgctat
     5281 gaaatcccga agaagaaata ccaatgagaa catcccctgg cttgatattt tttaaaggca
     5341 acgccttctt tttatcaacc gtccccaccg caaaaccggc cagatcatat tcaccggctt
     5401 catagaagcc cggcatttcc gccgtttcac cgccgatcaa ggcacagcct ccttgaaggc
     5461 agccttgcac gatccccgct aaaacttctt tcgctgtttt tggctttaac ttgcctgtcg
     5521 caaagtagtc caaaaagaat aaaggtttgg cgcctacaca gagcaaatca ttcactgaca
     5581 tggccaccag gtcgatgccg acggtatgat gggagtccca ctcaaaagcg agttttagtt
     5641 tggtccctac tccatccgta ctggccgcga gcaattgctt cgcgttcaaa gaataaagcg
     5701 aggcgtaccc ccccactgag gatttcacat tggcattgag agtggacttt accatcatcg
     5761 aaatgcttcc cacaaattgg tcagccaaat cgcgattcac tcccgctttt tcgtatgcgc
     5821 ctttttctga tttcatcttc taatgcctat tcttaatgtc tgaagtggcg atagccagtg
     5881 aaggccatgg cgattttatg ttcgttgcaa gccgcaatga ctgtcgcatc cttgatactt
     5941 ccccctggct ggacgatcgt gcgaatcccc gcacgggccg ccacatcgac agaatctctg
     6001 aagggaaaaa aagcatcact gattaaaatg cactccccga ggtccgtttt atttaaaaca
     6061 ctctgcgcgc ggggaatagc caacttttcc atggcctcga cacggttcgg ttggccttgg
     6121 ccagctccca ccatgcgata gtctccttcg ctgtcttcac aaaccaagca aactgaattg
     6181 ctcttgagag ttttattgca gaaaattcca aaatgcgcga gggcattcca ctgggtttgc
     6241 catggtactt cggtcactcc tttaagcggc tcttcgatct gttggtccaa gctttgggtt
     6301 aaggtgccac cggccacggt catctgcatg gaaggagaat ccaagtccca atgcttcact
     6361 gtcaccgctt ttaaattctt tctttgagtc agcagatctt taagatccgt gtcttttaat
     6421 ttgggtgctg aaaccaattc aacaaagcga ctttggaaat actgtagaat ttccgcatcc
     6481 acttcgtccg tgaaaattaa aacaccgccg aaggccgata cggggtcaca agcccaggcc
     6541 ttttctagtg cacggagttg cgctttaggc gcggactttg agaccgtggc aacgccacaa
     6601 gggttgttat gctttacgat caccacgctg gttttgtccg gaaaagccgc tttcagatcg
     6661 cgggccacaa aataagccga agaaagatcc acaatgttgt tataagaaag ctcagtggaa
     6721 gtcagtggat gattccaatc caatggagaa ttcgcatgta ctttcaatga agccttctga
     6781 tgtgggtttt caccgtagcg taaaaccttg gtgcctgcgc caaagacgga ttgaatactc
     6841 aaatcatagt ccgtcaccaa atcccaagct tgagccgcga cttctatggc ggtttttctt
     6901 tccaccgcgc cttgagaatt caattgctca atcaccgcag agtattgatt gggatccgtc
     6961 agcaccacca catccggagc attcttcgcc gctgcacgaa ccaaagcagg cccaccgata
     7021 tctattaatt ccgtcagttc atagcgggaa agattcttat ccatattctt ttggaaggga
     7081 tagaaattca ccaccaccgc atccaagagt ttgatattca acttttccca atccaattta
     7141 tcttttggat cttcccggcg agctaaaatt ccggagaaaa ttttaaaaga cagagtcttc
     7201 atacgccctt ggaaagcctc gggagtttca gacagctgtt ccgccgggcg acaaggaatt
     7261 ttagcatctt gcaaagtttt gagagttccg ccggaagcat aaagctccgt tcccgtttta
     7321 tgcatcgctt gagctaaatc aaccaggtct gttttatcag aaacactgag caaagctcga
     7381 cgaattttta gcggagcagt cgttacagac atgaggcctc cttataggca tttttaaaga
     7441 acatcattcc gatttcctct tcatgggatt tttctggaca caaggtccac tgagggtttt
     7501 gagttttacg aaccgccgcc tccggatggg gcatcaaacc aaagactcgg cctgagggat
     7561 tggtcaaacc cgcaatctgc tgggtactgc cgttcacatc ttcgctataa tacaggctga
     7621 ctttaaaatc gggacaattt gcaacatcca ccgaagaatc gaaaagcaaa cgtccctcac
     7681 cgtgacgaat cggcaaatac atcttttcaa gatctttcag aaagacattg gcagaggcgt
     7741 cttttttacc agccacttta agctccacat ccagctccac ccagcgattg ataaacttat
     7801 tctgaatatt atgcgtgata gaaacttttt taccaaaagc ccccatggcg atcaaagctt
     7861 gaaaaccatt gcacactccg agaacgatcc cgccattggc ggaataatct tcgagattcc
     7921 aattgaggcc cttggtcagc ttcaaagcca gcacccggcc cgaacccaaa tgatctccga
     7981 aggaaaaccc ccccggcata aagagcgcgc tgtattcttt tttcagatgg tcctgggaaa
     8041 acttcttttg aatcaaatca ttgatatgga caatatcaac tttaaatccc gcagcgcgag
     8101 ccgcatggga catttcttgt tcgcagttga tgccgtctcc agctaaaact aaagctttaa
     8161 tcataaactc tcccggctcc aagcttggta tagttcttcc acggcgatac tgtgagactg
     8221 atcttgaaac gtccatttcc actctgctgt tgcagttaaa ctgccgacct ccacaaagtc
     8281 gagggctttt tgtttccaaa tcttttcagc ggtcgcggct ttttctgcag ggacactgac
     8341 gataaattga tggaagcctt cgccgaaaga agttttcgcg agatggagtt ccaatgcgga
     8401 actaaactcc aaacctaaat tattggtgaa gcaactttcc gccagagccg tcaaaaggcc
     8461 gccctcactg aggtcatggg ctgagcgcaa atattcgctg gattctttag atcctagcca
     8521 ctgatataaa ttcaaagcct tcggccagtt tggagctgga gctgtcaccg ccttcacttc
     8581 actcttcgct tccgcatgca agccctgcca ctcggattga gccaagccca actgagctgg
     8641 tcctaactta tagactttat ctccggcttt cttaaaatcc gcggagcgag ccaaagtata
     8701 atccggcatt ctacccatgg cggtcaccaa caaggtcgga agcacagaga tctttaccgg
     8761 tttgccattt tggctgccct tgtaatcatt cttcatgctg tctttgcccg aaatcagcgg
     8821 agcctttaac gccagacaag cttcacgcag tccgtagcaa gttctgacca gagccgccat
     8881 tttttcgaca tcctcgaccg gatcgggcca acagaaatta tcaatcaaag ataaaaccgt
     8941 ttcatcgccc aaagaaaatt cagcgcccac gcaaagtaaa tttctcacgg cttcatccac
     9001 cgcagcttga gccataatat agggatccac tgcggacaat cttggattca acccgcagcc
     9061 caccacaacg ccgatagaat tattcgactt gagttccgtc gagagcgcta aaaccgccgc
     9121 atcattcgga gtcgatcttt gggttttaga agtttgcagg gacttcacca ccgaacgccc
     9181 ttgcacctca tgatcatatt ggcgaatgag attctctttg ctgcgaatat tcggccgctg
     9241 caaaatcgcc ttcagagttt ggaaaggttt ttcttcccgg ggctccgtcg ctcccgctgg
     9301 cgcagctttc tctacccaat gcgctttgag ctgcaaggga ggaacccctt catgcaaaaa
     9361 ttcaaggctc aaatcagcga ctttgctttc ggcatagaaa atttcaaagc gtccgctatg
     9421 ggtgaattca cccaaaacac tgacctcaac accacggcga tgggccaact ccaagaacgc
     9481 ggctttgttt tttggtggaa cagcgacggt cattctctct tgagattcac tgatgaccaa
     9541 ctcaaaaggt ttcagtccgt gcattttggt ttttgccaag gacacatcca tgcgcgcgcc
     9601 accggcaata tccgccatct ctccgacgga agaagaaagt ccccccgctc cgttgtcagt
     9661 cagcgtgcga tagagtccca aattacgggc ctcgatcagg aaatccgtca tacgacgttg
     9721 cgtgatagga tcgccgagct gaaccacgct ggaaggaata gactccgtca tttccagaga
     9781 ggagaaagtc gccccatgaa ttccatcgcg gccgatgcgt ccgcccacca tgcaaatcaa
     9841 atcgccattt tcaatttttt tctcggcaca cagtttgtcc gcgatcttcc gtggcataat
     9901 cccgccggag ccgcaaaaaa ccaagggctt gccaacaaaa gagggatcga aataaagagc
     9961 cccgttcacg gtgggaattc cgctctgatt gccgccggcc tcaactccgc tgcgaatgcc
    10021 ttcgaggatc tgcgctggag gaataattcc gcttggcagt ccatccgtta actccaaagg
    10081 accgacacag aaaacatccg tattgaaaat gggttttgcg ccaaagccgc aacctaaaat
    10141 atcacgattc acgcccacga ttccggtgat ggctccgcca aaaggatcca aagcggaagg
    10201 ggaattgtgc gtttcaactt taatacacac agcctcttct tcactgaaag ccacaatccc
    10261 cgcattgtct ttaaacactg atagcagcca aggctttggc aattccttcg tggtgccggc
    10321 gatggtggtt ttaaaaaggc tggaaatttt cgagggaata ttggactttt tcgaagcttc
    10381 ttctgagtat tccacctcag ccgaaaagat tttatgtttg caatgctcag accaggtctg
    10441 cgctaaaatc tccacttcca catcggtggg atttcttttc tctttggtaa aataattttg
    10501 aatcgcacgc atctcttgca gattcaaaga cagcagattt ttcttactca gctctatcaa
    10561 ctgatcatcg ttcatgccca gaataggaac gatttgcccc tgaggagtcg ttccggtagg
    10621 gagaataaat ttaggtccga tttctgaaga ttcaaaacga ggatactttt cgaattcact
    10681 tcgagagtaa acgcgatgac ggtgaatcag ggaattcgaa atcaagttag aggttatctt
    10741 ttccatatct tctttgacca attcaggacc cacaaacaaa taagcctgac ctgaaaccac
    10801 ttggctttca agcagctctc tacggcagca aatttctaaa gcctcaagtc cggtgcggcc
    10861 caaattatcg gtcatgccag gatgatattc gcgctccacc acccaatgac tttcgggcca
    10921 gtgcttttgt tgaagtactt catcccacca gatatccttg gcggcctcag tgaactcagc
    10981 actctcccct tcgaacttgg tccaaaaaag atccacaaca cgaagagatt tgattttttg
    11041 agagagcaca ggattccact tttggattct cgccaaaagg gccattccct tgggatcttg
    11101 cagttcattc ttaagaatga cttcggtgcg gttgaacatg aagtttctct ccttttttta
    11161 taatttcaaa ttgattttgg agaacccatt gcaaggtctg cggatacagc tcgtgttcaa
    11221 cggcaagtcc ccggcgttcg acgtcttcta cactgtgaca gtctgaaatc gagaattcac
    11281 gttgcgcgca aataagtccg gtatccactc cagcatccac aaaatgaaca gtgatccctg
    11341 ttcgcgataa cttccctaat aatttttgct caaaagcttt tttataactt tccagcccgg
    11401 gaaactccgg aagtaatgag ggatgaatat tcacgattct ggcaaagccc cggggatcgc
    11461 taaaggcact gataaattcc gctgaaagaa ttctcataaa ccccgcaagg accaggtaat
    11521 ctaaatttaa ctcttgaaga gtcgatttca tttttttttg atttttttcc accaaggttt
    11581 ccagccctcg cgctttggct ttttctaaaa ccaaagactc tggtcgatct gaaatgacca
    11641 cggcaatccg cgcatccaaa agttttgctt caatagcgtc agcaatggct tcaaaattag
    11701 agccgcgacc tgaggctaag atagcaattg ttggcgatgc gtttagaccc actcagaccc
    11761 cacgccctca cggtattgca ttccttcaaa ggaaatttct ttaagatggg caaaggcttt
    11821 ttggcgggcc tctttgatgg agcttcctaa accaagggct ccgagaactc gtccaccgga
    11881 ggtcacccac tcagccgcag gctgaccagc taactgagcc gcaggctgac cagctaactg
    11941 agccgcaggc tgctttttca caccggcaaa aaaaccatga tgcgattcaa gccaacgatc
    12001 cattccccga atcggttgcc ccaatgcagg ttctgtggga taccccggag ccgccgccac
    12061 cacataaacc gctacttgcg aagacatcgg caccatgcgg ggcatctgat gaagtgtgga
    12121 gcgagcacag gcttcacacc actccaaaag atctccctcc atcagaggca acaaggcctg
    12181 ggcttccgga tcgccaaagc gagcattgaa ttccaagacc cagaaatctt ctccatcaat
    12241 catcaggccc gcatacaaaa ttcctttgta aggggtcccc ctttttttca tttcttggag
    12301 ggtcggtaaa aatacggatt cacgaacttt agccgccaaa gtggaatctt cgaggggcac
    12361 agggcagacc gcacccatac caccggtatt cgagctcaag ggatcttctg tcaaagtttt
    12421 ataatcccgg actggagcca gaagcgaaca ggtctcgccg tcgcaaaaag ccaaccagga
    12481 aatctctttt cccttcaaac gctcttcaat tacaaaagat ttggaataaa cttggaggcg
    12541 ttccaaagct tgcagagctt gctcttgagt ttcacaaatc tccacgcctt ttccgagagc
    12601 caggccatcg gccttcatca cccattgctt tttttgccag ttcaaatcaa gaagaagttg
    12661 tttggcctcg gccacggagc gcacatcata gccctctgcc gtgggaactt ttgccgcttg
    12721 catgactttt ttagcaaaga ttttactgct ttccagacga gcggccgctt gacggggacc
    12781 gaaaatttcc agtccttctt tttcaaaaat atccaccgcg ccgtccgcca aaacattgtc
    12841 aggaccgacg acgacaaaat taactttttg ttctttcgct tttttcgcaa gcttggcaag
    12901 ggcttcaggg cccttcacag aatcggtcca agccgtaacg gatttattcg cgtactgatt
    12961 ttgcaattgc tgaacggccg ccgaattttc cggagccatc actaaacttt gaacccgcgc
    13021 cgatgccaaa agcttccaag ccatggcatg ctctcggccg ccggacccta gcaacaggat
    13081 tttcattcag aggctccttt aagataagtg gccaactccg tcaccacatg atccaaactc
    13141 ggacacggga ataatttctc agcggcaatt tcattcacca acgcttgata catttcactg
    13201 accagattct ttaaaaccgg cggcaaggct ggaggaggtt tcaccaaggc tttccagtca
    13261 gcataacctt tggcttctcc ctcttccttg gctttttcaa gctcctcata ccaaggagtc
    13321 tttctgtaga aatcgcgcaa gacttcttta gaaagttgca ttccctgttt ttccaaacgc
    13381 atctcgtcgg gaccaatggc atcgaccagg atgatttcgc cctccataga caaaccccac
    13441 tcgagttttc catccatcaa gcgaatgccc ttgtcgccaa accaagtttt aagggtttgt
    13501 gcgacctccg cagttttggc gctcactgcc acaaactggg ctggagtgag accgctgatt
    13561 ttgagagctt cttcctgaga caaaggacga tccttgtctt caagcttggt aaacatctcg
    13621 atgaagggtt ttgggaattg atcccctact ttataatttt tctcggcatg tcttttaata
    13681 aaagaactgc ccgcacaaac ttcaaaacgg aacaccactt ctaacggaat aagaaagggt
    13741 ttttccatcg tcttaggata ggtgtaataa ttccggccat tttcactgat tcgttccgga
    13801 cggtaaacgc cgatcttttg caccagaaca tttcgttcgc catcaaagcc gcggtaatga
    13861 gttttcacgc cttgagcttc aagtctttta aacaacacag acatcagttt cgctaaaccc
    13921 acccctttaa agggaatggt gtcgggcatt tttccccagt caaaaaccga gtagtcatcg
    13981 gtgaactcaa aaactagatt tgcagactca tccgaagaaa agattctttt caccgaacct
    14041 tcatacatca agcgattctt gctaaatcta tttgtaagca ctccggtcct catttcttaa
    14101 ttgaattaat tctttcaccc cctcaatgcc tgtcggatat tgtttgttca aacaggccat
    14161 gcaaggggtt atattgtttt cagggaaaat ggcttctttc aaggcttcca tttcctggta
    14221 gataacgcca tccgcaccca attcttcttc gacgctttgc agggttttat tgttcgcgag
    14281 aagttctttc tccatgggga aatcgattcc ataaaaacag ggatgcttga tcggagggca
    14341 ggtcgacgcg aaataaactt ctttagctcc cgccatacgc accagctcaa tcaattttcg
    14401 cgaagtcgtt ccgcgcacga tggaatcatc cacaagcaaa acccgtttgt ctttaatctc
    14461 ggaatagacc ggcgccaatt tcaaatgcat ggcctgctcg cgtttgtcct gactgtctaa
    14521 aataaaagtt cgattaatat aacggttctt aatcaaaact tcacgataag gaacttttaa
    14581 ttcttcagac aaggcaatcg ccgcgatacg cgcggtttcc ggaactggaa caacaatatc
    14641 aaatccaata ttgtctttta acatgcgctc acgcactgaa atcgccattt tttttcccaa
    14701 ctcaatccgc gccttataaa ccggacttcc ttcgatttcc gacgtgggag aggcaaaata
    14761 aacccactcg aacatacacg gtcgatgggc ctgggcatac agctgttttc tttgcatccc
    14821 cccatccatg gaaatcgcta cgatttctcc tggccggata tcttcaaagt tttcgtaacc
    14881 caagaaagac agcgcggtgc tttcagaagc caaagcataa gaaagcttcc cgtcgtcttc
    14941 ctgtcgtgtc cccatgacta agggacgaag accatgggga tcacggaaag ccaccaaccc
    15001 aaaacctgcc accatcgaaa caatgctgta acttcctatg gccttctcga aaataaaaga
    15061 aaccgaggaa caaaaatctt caaagctcgg cgtgcgttta tggacatttt ttgccaggcc
    15121 ttcagcaaag agattgataa tattttcggt atctgaatcg gccagaggct ggcgttgata
    15181 ttttgtgcga agttcctgcg tcagcgctcg gaaattgacc agattgccat tatgaacgag
    15241 gccaatccca taagggtaat tgagtaaaaa aggctgcacg ttccgcagct cacttttccc
    15301 ggcggttcca tagcgaacgt gaccgatagc cacagttcca gtgagagggc gcatattttc
    15361 ctcagtgaaa actttttcca ccaagccgag atttctcaca tagtgaaacc cggagtcatc
    15421 ataagataag atccccgcgg catcttgacc tcgatgttga agtaaactga gacccctcag
    15481 cgctcttgag gaagcctggc tagtgccaat gagcccaatg actccgcaca tcgtccctcc
    15541 gaacaggtgc taaacaccca ttttatttac attttttgaa tgcatgcacg ctagcagaag
    15601 ggcttcttaa tataaagaaa aaaattcaag atttcgttaa gaccaggggc gccatcaaca
    15661 ggactgttta gcctctttct gaagggcctt taaaagggcc ttgactcttt tcgagaggat
    15721 agcccctcat agatcttcga tgaaaacaaa ctcttaaaat attaaacctt catgaagctg
    15781 ctgaagaacc cgctaaagca aattgatatc cttcttcaag aagaaagtta actagacttc
    15841 ttcagttaca cccccaacat tccacatatc ccttgcttta tgcatataaa atcattaacc
    15901 tatatgcata ggaaggagcc ctcatgcgaa ccactctcga tttgaacgaa gatttagtga
    15961 accaggctcg taaactcacc aatataaaag aaaaaacggc cctgatccat gaggctctcc
    16021 gggtccttat tgcccaggcc gctcaaaaag aacttgctct tatgggaggc acgcaaaaac
    16081 aggtaaaacc tattccccgt cgtaaagccg gataggttat gatacttgtt gataccaatg
    16141 tttggattga acacttcaaa gtttcttcgc cgcaacttat agaactcctg atggagggaa
    16201 gggtcgtcac tcatgagttc attctcggag agctgtcact cacccatttt aacaaaaaag
    16261 atcgtgaaaa aattttcgaa agactgcgag ccttggaaag agtgaaaacc tcaaagcatg
    16321 aagaggttta tgatttttca tttcactcta aattgtcgga aaagggaatt ggctggattg
    16381 atagccattt actgcacgcc tgcgtcaccc aaaagctcga acttttgacc ttcgataaaa
    16441 acctatctca gctagcgaaa aaaataatca gataacggcg aattctttct tgccttactt
    16501 caaaacagct ttcaaaaatt ttccggtctc actatttttg actttgacga tttgttctgg
    16561 tgttcccgtc gcaatgataa gccccccggc tttaccgcca tcaggtccca gatcaatcac
    16621 atgatccgca cacttcacca cttcgagatt gtgctcgatc accagcacgg tatttccttg
    16681 atgactcaga tcttgaatca gttccaccaa ttttttgatg tcttcaaaat gcaaacctgt
    16741 ggtgggttca tctaaaatat acaatgtctt tccggttccg cgacgagaga gttctttgga
    16801 taatttcacc cgctgggctt cgccacccga caaagtcgtt gaactctgac cgagcgtcat
    16861 ataatccaac cccacgcgat tcaaagtttc gagtttgcga taaatctgcg gatggttttt
    16921 aaatagttcc aaggcctcca agacgctcat attaagaata tccgaaatgg actttccttt
    16981 gtactttacg ttcagagttt cacgattgta acgagcgccc agacaagttt cgcatttcac
    17041 gaagacgtca ctcatgaaat gcatttccac gcgaatttgc ccatgtccca agcaggtctc
    17101 gcaacgcccg cccttcacgt taaaactaaa acgtcccgga ggataacccc gcagttttga
    17161 gtccggcagg cccgcaaaca aatcacgaat catgggaaac aaccccacat aggtggctgg
    17221 agttgaacga ggcgtgcgac caatgggtct ttgattgata tcaatgacct tgtcaatatg
    17281 ctcaatcccc tccaactttt gaaaactcga aggcgaggca ccggctttgt aaaagcgttg
    17341 ggccaggatt ttaaaaagcg tatcaatgat caaggtgctc ttgccactgc ctgaaactcc
    17401 ggttaccgca gtaaaagtgc ccaggggaat ttccagcgtc acatttttca agttgttgcc
    17461 actggctccg atcaaacgca atttctgacc attgccttga cgacgttgcc tcgggatcgg
    17521 gattcgcagc tctcccgaaa gatagcgacc cgtcagactt tttggattgg cctcaatttc
    17581 ttgcggagtt cccgccgcca tcatctctcc gcctaagcgc cctgctcgag gacctaggtc
    17641 gacaacatga tcggcgaaac gaattgtgtc ctcgtcatgc tccacgagca aaatggtatt
    17701 tccgcggtcc ttcagctcgg caatgatatg caacaggcga tgatgatcgc ggggatgcaa
    17761 acctattgaa ggttcatcca tcacgtacaa aactccaatc aatgaagatc ccacctgcgt
    17821 tgccaaacga atccgctggg cttctccgcc ggaaagagtt ctcgaaggac gacttaaaga
    17881 caaatatccc gttcccacac gaattaaata atccaggcga ttgagaatct gcttggaaat
    17941 cttttcggcg atcatctgat ctttggattt ccaagaaact ttggcgagcc aatcccgcaa
    18001 ctccacagca cttaaatccg aaagctcggc gatattttta tcctggaggc gaacgttcaa
    18061 agattctttt ttaagccggg ccccctgaca atcggggcac acggaaagaa ccatctcttc
    18121 actttcttct tcttcatcat ttgatgaagc caccttttta cctttataag tgtattttac
    18181 tttctccaga cgacgagccc caaccacgcc ctctgaaaac tcttcttctt ccaccagatc
    18241 cgcagtgcca agtccatggc aggtcaggca agctccacgg ggattgttga aactgaaaat
    18301 gcgcggttcg atttccggaa agctgtaacc gcattgaggg caggacgaat gcaaactgta
    18361 agacactcgt tccccattca aagtctgggt caccaaacgg ccattggcca tgcttaaggc
    18421 cgtattgata ctttccgcca aacgcagccg attcacagag tcttttaaaa tcacctgatc
    18481 cacgaccagg tcgatatcat ggactttggt tttcgcaagt ttggtggctt tttccaggtc
    18541 gatccatttt ccatcaatct gtgctttgac gaaacctttg cgcgcccaac gctgaaactc
    18601 agccatgaat tcgccttttt taccttgagc cattggagcc aaaatatgaa attttgcagc
    18661 ttgtggattc ttagaacctt tctttaaaat ttcctcgaga atttgctgag gcgtctggct
    18721 gctcactgga agatgatgcg tgggacactc cgggaccccg acctttgcaa acaacaatcg
    18781 catataatca tagatttccg tcaccgtgcc caccgtcgag cgtggatttg tactgacgga
    18841 tttttgatca atcgcgatgg caggagaaag tcctgtgact gaatccactt ctggtttttt
    18901 taattgttct aagaagtttc gtgcataggc cgaaagactt tccacatagc gccgttgacc
    18961 ttccgcatac acggtatcaa aggccaaact ggatttgcca ctgccgctga ggccggtgaa
    19021 aaccgtgatt ttattgcggg ggatgaccac gctcacatct ttgagattgt gttctttcgc
    19081 acccttgaca acaataccgt cgaattcttg attagaagcc atctcgccac tttacaaaaa
    19141 cccgcaacaa ggcgcaagaa gtgctttggc gcgggtcatg tgatctaaca gctatttcat
    19201 ttggcagcct ggacaaactc cgtaaagctc cagaacgtgg tgggtcaatt ggtaaccgaa
    19261 ttgttttgct actttttctt gcaaagactc gatggtttta ttctcgaatt cacagatttt
    19321 gccacaccga acacaggtta aatgatcatg gtgatttttt aagttcagtt cataacgcgc
    19381 cggcaatccg cccatgcgca cttcagcgac gaaatgcccc tcactcaagg ttttcaaaaa
    19441 acgatataca gtcgcaaagc caatttcagg atgagcggca tttactctct caaagagttc
    19501 ttgtgcggta atatgacgat gcccctcatg caaggctttc aaaatcgcca ttcgctgagt
    19561 cgtcaccttg agattcagtg cacgaataat tttttttaat tcagacttac ccacttcatc
    19621 accgtaaacg ataatgtcct catcatgttg gcgcggcagt aagggaactg tatctttgct
    19681 cattggcacc tctctgcttt aaaggtataa gcaaagctcc ggccaaagtc taatcaatta
    19741 agaatagttc tcacttgtag ccttaatttt tgaagtaatg aaagccaaat gacagctgac
    19801 aaatatttga ttttaaacag aagtccatag attttattat aaaaatgaga ccgccgccct
    19861 aacctgtaac ggttcttcgc ggaggcttgt atgataaaat taatttcact ttttctgctt
    19921 gtactgtctt ttgcttatac cagtttagct gatccagatg tgcctctccc aggcccagga
    19981 gctccaccga gcgagccccc tgcgccacaa ccgacgcctc ctccgtcccc cactcctccg
    20041 tcccccactc ctcctccacc ggattctccg tcaccgaaac caacgccgaa accacgccag
    20101 tctccgcgaa agccccccag acgaactcca aaggtgcctg cgcccacacc cactccagat
    20161 ccggatcaag atttaacccc gacgccgact ccgtggccga gcgaaacccc caccccatca
    20221 ccaattccac cgcaggaatc taaaaaaact tataccgtag gagttggcca ggtagcccgc
    20281 tttaaagacc gtgttttcaa tttttttatc cctgcgcatc ggagtagtat tcgcgccctc
    20341 cgtatcacct gtacccgcca agaaattaaa atcaagaacg tcactgtcca atttaccaat
    20401 ggtcagagat ctgatcagtt ctttttggtg ggcccgctta attttggaga gagccgagtc
    20461 acctatatgc cgggcgaggc tgtctttaaa attaccgtag tggcttcaag ctcttcgatc
    20521 tttaaaggca agggtgcctt tagattagat gctacggcga tattacttcg ccgcagaaac
    20581 gaagggaaag aggaacctca ccagatagaa tctcccctgc ttgatgaaaa caaggaaacc
    20641 ccttattaaa ggcccccctt gtttaaaata aaaattaagg gcaacgttgc aaattgttaa
    20701 tcagatcata gatggaatta cgagca
//
LOCUS       DATBTE010000183         8578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_910419, whole genome shotgun sequence.
ACCESSION   DATBTE010000183 DATBTE010000000
VERSION     DATBTE010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8578
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910419"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VIG33_06740"
     CDS             <1..823
                     /locus_tag="VIG33_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY8270569.1"
                     /translation="IFMQRKGLGAVFRIIPEKIKTAQELNLPNSVMEVIDCEKGLILV
                     TGPTGSGKSTTLASLIHHINATREAHIITVEDPIEFVHPNIKSLINQREVGSHTKSFA
                     NALKAALREDPDILLVGELRDLETIALALTAAETGHIVFGTLHTNSAAKTIDRIIDVF
                     PSSQQQQIRTMLAESLRGVIAQTLFTRADGQGRIAAYEIMRNTRAIANLIRDNKIHQI
                     PSAMQTGSSSGMILFEKYIEDLVRKGKVSTHDAKTFLGKDAEATSVNSGNGTKAG"
     gene            883..1548
                     /gene="deoC"
                     /locus_tag="VIG33_06745"
     CDS             883..1548
                     /gene="deoC"
                     /locus_tag="VIG33_06745"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEY8270570.1"
                     /translation="MKNLASYIDHTLLKPETTELHIRQLCKEALENHFFSVCINTSFI
                     PLCKELLKNSQIKICAVIGFPLGAMSTDAKAFETQWAIQKGADEIDMVIQVGFLKNHQ
                     YDYIYNDIAKVVEAAQNRPVKVIIETSLLSHEEKIKACQLSKDAKAHFVKTCTGFAGG
                     GATVEDVLLMKSIVGDAMEVKASGGIRNLASAEALLKAGATRLGTSSGVQLVKNQTAS
                     GAY"
     gene            1549..2850
                     /locus_tag="VIG33_06750"
     CDS             1549..2850
                     /locus_tag="VIG33_06750"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEY8270571.1"
                     /translation="MSFLASEIIKIKRNGGILSSEHIKEFILGYSRGRIPDYQMSALL
                     MAIYFKGMTTEETLSLTKAMLHSGEVVDLSQLSGFKVDKHSTGGVGDKTSLILGPIVA
                     TAGVTVPMISGRGLGHTGGTLDKLESIPGFNTQLDLLTFKKMVAQHKICFIGQTKEIC
                     PADKKIYALRDVTATIESLPLICASIMSKKMAEGIDGLVLDVKYGTGAFMKRPEEAEE
                     LAVNLMSIARGYGKKVVALLTNMNQPLGRYAGNSLEVEECVDIMKGKEHPRYQDTREL
                     SLWLSAHMLHIAGRTKTVEDAYKLAAEILTSGKAFQKFEELCVIQGGNLSALPKPKNK
                     TVITSEKAGFVSGFNTESIGVAGILLKAGRAVATDLIAPTAGIEFHVKVADSVQVGDP
                     IYTIHGDDLELMQSALPLLKSSITISLQKPSSPSLILKTLV"
     gene            2858..3673
                     /locus_tag="VIG33_06755"
     CDS             2858..3673
                     /locus_tag="VIG33_06755"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEY8270572.1"
                     /translation="MVFQKLTETVKYIRSVTPCKPRVGVTLGSGLGAFVKELEVEHTI
                     PYHEIPFFMPTTVEGHQGNLIFGKIAGQSIVVLQGRNHYYEGHSMETVVFPTRTLALL
                     GIESLILTNSAGGFGETMQAGDFMIIEDHINLMGANPLMGPNIKELGPRFPDMTEAYD
                     KKLIEKMEMVFQKQKTRYHKGIYCGVSGPTYETPSEVRYLKLIGGKAVGMSTVPEVIA
                     ANHLGLRVAALSCITNLASGISRQKLSHQEVTDTAKQVEKQFTDFLKEFVQLI"
     gene            3855..5096
                     /locus_tag="VIG33_06760"
     CDS             3855..5096
                     /locus_tag="VIG33_06760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY8270573.1"
                     /translation="MDVGIRSLSRVLVLLFLISACSPKTTTSVFDEKNYGSSACSGQA
                     LNTRYVVQWENGQFTVESATDVEKFKKDFLEPNLDKIRKVEYDRVLQLKKRTDVVSAA
                     SVDTDAEWGQNIVQVSTLWNQGIYGQNVLVGIVDSTVDINNVQLSGNIAYNTGEIPGN
                     GLDDDENGYVDDYAGYNFISSPNNSKVVSDHGTHVSGIIAADQTKGSIKGIAPHAKII
                     PAPFIDGANGGSIGDAILALQYVSTRGAKVINASWGGAPCMLSLQNAFAELNKKGILI
                     VVAAGNEGADIDYIPDYPAAFNMPNQLTVAASTSYDFMASWSNVGFTLVHLAAPGNDI
                     LSTITGNRTSFMSGTSMAAPFVTGAAALLWSHRPQATVSDIKTALLRSVDVTPNHEFK
                     VQTMGRMNVKKALEELNKLLP"
     gene            5187..6476
                     /gene="asnS"
                     /locus_tag="VIG33_06765"
     CDS             5187..6476
                     /gene="asnS"
                     /locus_tag="VIG33_06765"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEY8270574.1"
                     /translation="MRYYISDLKDHVGQKVELKGWTYNTRSSGKVKFLELRDGTGLVP
                     CIFFKVDCGDAAYDLFDKMTQESSVRVVGVVREHPKRKGEFEVGVTEAELLGTSENYP
                     ITPKEHGTDFLMDNRHLWIRSKRQHAILRVRHEIIAAIRDFFDSRGFTLTDAPIFTPA
                     ACEGTSNLFETKYFDEKAYLSQSGQLYMEATARAFGKVYCFGPTFRAEKSKTRRHLIE
                     FWMVEPEVAFNDMYDNMELAEQFVEYIVQRVLKNRADELKVLERDTSKLAPIIAPFPK
                     LHYKEAAEIILKENPNFVVGDDFGAPDETIVSSKFDKPVFVHHYPQAVKAFYMKEDPK
                     EPGYAMGSDLLATEGYGEMIGGGQREESVEVLLKKIAEHGLNEKDFQWYLDIRRYGSV
                     PSSGFGLGLERTVAWICGLPHIRETIPFPRLYGRSYP"
     gene            6494..8056
                     /locus_tag="VIG33_06770"
     CDS             6494..8056
                     /locus_tag="VIG33_06770"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEY8270575.1"
                     /translation="MEETNTLQQRLFDLEKRNEQAMLGGGASRVAKHKQGGRLAARER
                     LDILLDPGSFVEMDRFVSHRCINFGMDKTVVPGDGVVTGYGRVNGKLVYVSSQDFTVI
                     GGSMSRTQANKICKVMDLAAKNGAPFIALNDSGGARIQEGIESLGGYADIFTRNVMCS
                     GLVPQITAIMGPCAGGAVYSPSITDFVFMVKNTSYMFVTGPEVIKTVTHEEVTKEDLG
                     GATTHSSKSGVAHFATNDDKHCLLMIRELLSFVPSNNLDDAPTLPTKDTPDRLVEALN
                     TMIPDNPKKPYDMLNVITECVDEGYFLEVHKHYAQNIIVGFARFNGRSVGIVANQPNI
                     LAGCLNIEASRKAARFIRFCDSFNIPVVSFVDVPGFLPGKDQEWNGIITHGAKLLYAY
                     AEATVPKITVITRKAYGGAYIVMGSKLLKNDVNLAFPSAEIAVMGADGAVSIIFREEI
                     NKAKDPTAERTRLTAEYEAKFSNPYVSAELGYTDEVIEPAMTRKRIIDSLEMLKNKRD
                     VMPAKKHGNIPL"
     gene            8079..>8578
                     /locus_tag="VIG33_06775"
     CDS             8079..>8578
                     /locus_tag="VIG33_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY8270576.1"
                     /translation="MQKPFKKILIANRGEIAIRIIRACRELGITSVAVFSDADRDSLH
                     VFLADEAYHIGPSSSKESYLNYRKILEVAKTAHADAVHPGYGFLSENTIFARACNEAG
                     INFIGPTPENIDAMGDKISSKLLMQKSGVPTVPGSDGGVETVEEAMSIAERVGLPIII
                     KATAGGG"
BASE COUNT         2429 a         1843 c         1941 g         2365 t
ORIGIN      
        1 tattttcatg caacgaaaag gcctaggcgc tgtctttcgt attattcctg aaaaaatcaa
       61 aactgcacag gagctgaacc ttcctaattc agtaatggag gtcattgact gcgaaaaagg
      121 cctaatcctt gtcactggac cgacaggctc tggtaaatcg accaccctgg cctcattaat
      181 ccaccatatc aacgcgactc gagaagctca tattatcacg gttgaagatc ctattgagtt
      241 cgtgcatcca aatattaaat ccttgatcaa tcaacgcgag gtcggaagcc atacaaagtc
      301 ttttgccaat gcattgaaag ccgcacttcg tgaagatccg gatatcttgc tcgtcggcga
      361 gctgcgggat ttagaaacga tcgctttggc gctcaccgcc gctgaaaccg gacacattgt
      421 gttcggaaca cttcacacca atagtgccgc aaaaaccatt gaccggatca ttgacgtatt
      481 cccttccagc cagcagcaac agatcagaac catgctcgcg gaaagcttac ggggagttat
      541 tgcccaaacc ctgttcaccc gagcggacgg acaaggtcgt attgccgctt atgaaatcat
      601 gagaaatacc agagccattg cgaatctcat tcgtgataat aaaatccatc aaattccatc
      661 agctatgcag acaggatctt caagcggcat gatacttttc gaaaaatata ttgaagattt
      721 agttagaaag ggaaaagtct ctacccatga cgctaaaacc ttcctcggaa aagacgcaga
      781 agccacctcc gtaaattccg gtaatggtac taaagcaggt tagttttttg actttttaaa
      841 gaagcctcga taccttccaa tggacaacat tgggaggtcc ttttgaaaaa tttagcctca
      901 tatatagacc atacactctt aaaacccgaa accactgagc tacacattcg tcaactttgc
      961 aaagaggcct tggaaaatca tttttttagt gtttgcatta acacaagttt tatccctctc
     1021 tgtaaagaac ttttaaaaaa ctcgcagata aaaatctgtg ccgttatagg ctttcccttg
     1081 ggagccatga gtaccgatgc aaaagccttt gaaactcagt gggctataca aaagggcgct
     1141 gatgaaatcg atatggtcat tcaagttggt tttctaaaaa atcatcaata tgattatatt
     1201 tataatgata ttgcaaaagt cgttgaggcc gctcaaaatc gacccgtaaa ggtgattatt
     1261 gagacctctc tgctttctca tgaagaaaaa atcaaagctt gccaacttag caaagacgcg
     1321 aaagctcatt tcgttaaaac ctgcaccggc tttgcgggcg gcggagccac tgtagaagac
     1381 gtgcttttaa tgaaatctat tgtgggcgac gctatggaag ttaaagccag cgggggcatc
     1441 cgtaacctgg ccagcgccga agccctgctc aaagcaggag ctacgcgtct tggtacaagt
     1501 tcaggcgttc aactcgttaa aaaccaaact gctagcggag cttactaaat gtcttttctt
     1561 gcctctgaaa ttattaaaat aaaacgcaat ggcggcattt tatcttcgga acacattaaa
     1621 gagtttattc ttgggtattc ccgaggtcgc attcctgatt atcagatgtc cgctctctta
     1681 atggcaattt attttaaagg gatgaccaca gaggaaactt tgtctttaac gaaagccatg
     1741 ctccattctg gtgaagttgt ggatttatcg caactctccg gttttaaagt ggacaaacac
     1801 tctaccggtg gcgtcggtga taaaacgagt ttaattctag gtcccattgt ggccacagcc
     1861 ggagtgactg taccaatgat ttctggaaga ggacttggcc acactggagg caccctcgat
     1921 aagcttgaaa gcattcctgg atttaatact cagcttgatt tgctcacctt caaaaaaatg
     1981 gtggcacagc acaagatctg ttttatcggt cagaccaaag aaatctgtcc tgccgataaa
     2041 aaaatctatg ctctacgcga tgtcactgca acaatcgaaa gtcttccgct catttgcgca
     2101 agcattatgt ctaaaaaaat ggccgaagga attgatgggt tggttcttga cgtaaaatat
     2161 ggtaccggcg cttttatgaa acggcctgaa gaagctgagg agcttgctgt aaacttaatg
     2221 agcattgctc gaggctacgg aaaaaaggtc gtcgctcttt tgacaaatat gaatcaacct
     2281 ctgggacgct atgctggaaa ctccctcgag gtagaggaat gcgtcgacat tatgaaaggc
     2341 aaagaacacc cccgttacca agacactcga gaactcagtc tgtggttgtc agcacatatg
     2401 ctgcatatag caggcagaac taaaaccgtc gaagatgctt acaaattagc tgcagaaata
     2461 ttaacctccg gaaaagcttt tcaaaagttt gaagaacttt gcgtgattca aggcggaaat
     2521 ctgagtgctc taccgaaacc aaaaaataaa actgttataa cttctgagaa agctggtttt
     2581 gtcagtggtt ttaatacgga atccattggt gtggcgggga ttttattaaa agcaggccga
     2641 gctgttgcta cagacttgat tgctccaaca gctggaattg aatttcatgt taaggtcgcc
     2701 gattcggtac aagtaggaga tccaatctat acgattcatg gagatgattt ggagttgatg
     2761 caatcggcgc tgccattgct gaaatcctct atcactatct ccttgcaaaa gccatcaagt
     2821 ccgagtttga tacttaaaac tcttgtttag agggattatg gtattccaaa agttaactga
     2881 aacagtaaag tacattcgct ccgtcactcc ttgcaaaccc cgagtagggg tcactttggg
     2941 ttcaggccta ggtgctttcg tgaaagagct tgaggtggaa cacacgatcc cctatcacga
     3001 gattcctttc tttatgccaa ccacagttga gggacaccaa ggcaatctta ttttcgggaa
     3061 aattgcgggt caatccattg tagttttaca aggccgtaac cattattatg aaggccacag
     3121 catggaaaca gtggtttttc caactcgaac tttggctctc ctggggatcg agtctttaat
     3181 tttaacaaat tctgcgggcg gttttggcga aacaatgcag gccggagatt ttatgattat
     3241 tgaagatcat attaatctta tgggagctaa tcccttgatg ggaccaaata ttaaagagtt
     3301 aggtcctcgt tttccagata tgacggaggc ctatgacaaa aaactaattg aaaaaatgga
     3361 aatggttttt caaaaacaaa aaacccgtta tcacaaggga atctattgtg gggtcagcgg
     3421 ccctacttat gaaacacctt ctgaagttcg ttatttaaaa ctgattggcg gaaaagccgt
     3481 agggatgagc actgttccgg aagtaattgc ggcaaaccac ttaggccttc gtgtcgccgc
     3541 cttaagctgt attacgaatc ttgcctcggg catctcacgt caaaaacttt cacaccagga
     3601 agtgactgac accgctaaac aggtggaaaa acaattcacg gactttttaa aagaatttgt
     3661 tcaattgatc taaattaagc ttcatttgga aagcgacaac ctttaaattc gaagaacccg
     3721 actacaacta gaccttaagt ctagttgtct tggttcaaga ctatataaat ttcaaaagcc
     3781 tccgatgagg taaccgaggt aatccgcgta acttgtctca ctgatgggac caagacggtt
     3841 cctcgggagt caacatggac gttgggattc gttctctaag cagagttctt gttttactat
     3901 ttttaatatc ggcttgcagt ccgaaaacaa caacatccgt atttgatgaa aaaaattacg
     3961 gcagttctgc ctgctcggga caagccctca acacacgcta tgtcgtgcaa tgggaaaatg
     4021 gccaattcac agtggagtct gcaactgatg tcgagaaatt caaaaaagat tttcttgagc
     4081 cgaatctcga taagatccgc aaagtggaat atgaccgtgt cttgcaactt aagaaaagaa
     4141 ctgatgtagt ttcggctgcc agcgtggaca ctgatgctga gtggggtcaa aatatagttc
     4201 aggtttctac tctatggaac caaggcattt atggccagaa tgtcctggta ggtatcgttg
     4261 attccactgt tgatattaat aacgtgcaac tgtccggtaa catagcttac aacacaggcg
     4321 agattcctgg aaatggatta gatgacgatg aaaacggcta cgtagatgac tacgctggat
     4381 ataattttat ttcaagtcca aacaatagta aagtcgtcag tgatcatggc actcatgttt
     4441 cgggaattat tgcagctgat caaaccaaag gctcaatcaa aggtatagca cctcacgcaa
     4501 aaattattcc cgccccgttt attgatggag caaacggggg ctcgatcgga gatgcgattt
     4561 tagctcttca gtacgtaagc actcgcggcg ctaaggtcat caatgccagc tggggagggg
     4621 ctccttgcat gctgtcccta caaaacgctt ttgctgaact caataaaaaa ggaattctta
     4681 tagtcgttgc ggctggtaac gaaggagcag atattgatta tattcctgat tacccggctg
     4741 ccttcaatat gccaaatcaa ctgactgtgg cggcatcaac aagttatgat tttatggctt
     4801 catggtccaa tgttgggttt accttggtcc acttagcggc acctggcaat gatatcctta
     4861 gtactattac cggcaatcgt acaagtttta tgagtggaac ttctatggcg gccccctttg
     4921 tcaccggtgc tgcggcttta ttatggagcc atcgcccgca agccactgtc agtgatatta
     4981 aaactgcctt gctgcgctcc gtcgacgtaa ctcccaatca tgagtttaaa gttcagacca
     5041 tgggacgcat gaatgtgaaa aaagccctcg aggagcttaa taagcttctt ccatagcgca
     5101 ttgctgcgcg tccgaatttt caagtgagct tcctctcctc ctcatggtaa aaatccttct
     5161 ttagtttctt ttggaaaggg tttctcatga gatattatat ctctgattta aaagatcatg
     5221 taggtcaaaa ggttgaactc aaggggtgga cttataacac tcgttcttct gggaaggtga
     5281 agtttttaga acttcgcgac ggtacaggcc ttgttccctg cattttcttt aaagtggatt
     5341 gcggcgatgc tgcttatgat ctttttgata agatgacaca agaatcttcg gtgcgcgtcg
     5401 ttggagttgt gcgcgaacat cccaaacgca aaggcgagtt tgaagtcggc gtcaccgagg
     5461 cagaactgct aggcacctca gaaaattatc ccatcacacc aaaagagcat gggactgatt
     5521 ttttgatgga taaccgccat ctatggattc gctctaaacg tcagcacgcc atcttgcgtg
     5581 ttcgccatga aatcatcgcg gccattcgtg atttttttga tagtcgcgga tttacactaa
     5641 cagatgctcc tatctttact cctgccgcct gtgaaggaac ctctaaccta tttgagacaa
     5701 aatacttcga tgagaaagct tacttatccc aatcaggtca gctctacatg gaagccacag
     5761 ctcgagcctt tggtaaagtt tattgcttcg gacccacttt tcgcgcagag aaatcaaaaa
     5821 ctcgtcgtca tttaattgag ttctggatgg ttgagcctga agtggcattc aatgatatgt
     5881 atgacaatat ggaacttgcc gaacaattcg ttgaatatat cgttcagcgg gtactgaaga
     5941 atcgtgccga cgaattaaaa gttcttgaac gcgatacaag taaacttgct ccgattatcg
     6001 caccttttcc aaaattacat tacaaagaag ctgctgaaat cattttgaaa gaaaatccca
     6061 atttcgttgt aggtgatgac ttcggcgctc cagatgaaac aatagtttct tcaaaattcg
     6121 acaaacccgt tttcgtccat cactatccgc aagcagtgaa agctttctat atgaaagaag
     6181 atccaaaaga gccgggttat gcgatgggtt ctgacctcct ggctacggag ggttatggcg
     6241 aaatgatcgg gggaggccag cgtgaagaaa gcgttgaggt tctcttgaaa aaaatcgccg
     6301 aacatggcct caatgaaaaa gatttccaat ggtacctcga tattcgccgc tatggctcgg
     6361 ttcctagcag tggatttggt ctaggtcttg aaagaacagt tgcttggatt tgcggtcttc
     6421 ctcacattcg tgagaccatt ccgttccctc gcttgtatgg tcgctcttat ccttaactct
     6481 tattggagaa tacatggaag aaacaaatac tcttcagcag cggttgtttg acctggagaa
     6541 aagaaatgaa caagcaatgt tgggaggggg agcctctcga gttgccaagc acaagcaagg
     6601 tggacgcctt gctgcacgag aacgtttgga cattttactt gatccaggca gctttgtgga
     6661 gatggatcgt ttcgtcagcc atcgctgtat taatttcgga atggataaaa ccgtcgtccc
     6721 tggcgacggc gttgttaccg gctatggccg agtgaatggc aaattagttt atgtttccag
     6781 ccaagatttt actgttattg ggggctcgat gtcccgcacc caagccaata agatctgtaa
     6841 agttatggat ttagccgcta agaatggcgc tccgttcatc gctttaaatg actctggtgg
     6901 ggctcgtatt caagagggaa tcgagtcatt ggggggttac gctgatatct ttactcgcaa
     6961 cgtgatgtgt tcagggctcg ttcctcagat tacagccatc atggggcctt gtgctggcgg
     7021 tgcggtttat tcaccttcca tcactgattt tgtcttcatg gtgaaaaata cgagttatat
     7081 gtttgtaact ggtcctgaag ttattaagac ggtcacacat gaagaagtga ctaaagaaga
     7141 cctcggtgga gctaccactc actcttctaa atccggcgtg gctcactttg cgacaaacga
     7201 tgataagcat tgcttgttga tgattcgtga gcttttgagc tttgtacctt ccaacaactt
     7261 ggacgacgcc ccgactttgc caactaaaga cacaccggat cgcctcgtcg aagcattaaa
     7321 cacgatgatt cctgataatc caaaaaaacc ttacgacatg ctcaatgtaa ttactgaatg
     7381 cgtggatgaa ggttactttt tggaagtcca caaacactat gctcaaaaca ttatcgttgg
     7441 atttgctcgt tttaatggac gcagcgtggg aattgttgcg aatcaaccta atattcttgc
     7501 cggttgcttg aatattgaag cttcccgtaa ggccgctcgt tttatccggt tttgtgattc
     7561 attcaatatt cctgtagtta gctttgtcga tgttcctgga ttcttgcctg ggaaagatca
     7621 agaatggaat ggaatcatta ctcatggagc aaaacttctt tatgcttacg ctgaggcgac
     7681 tgtgcctaaa attacagtga ttacacgcaa agcctatggc ggagcttata tcgtcatggg
     7741 ttctaaatta ttaaaaaatg atgtaaacct ggcctttcct tccgcagaaa tcgctgtcat
     7801 gggtgccgat ggcgccgtga gcatcatttt ccgtgaagaa attaataaag caaaagatcc
     7861 aactgcagag cgcactcgct tgaccgctga atacgaggca aaatttagta atccttatgt
     7921 ctctgcggag ctgggatata cagacgaggt cattgaacct gcgatgactc gtaagcgtat
     7981 tattgactct cttgagatgc ttaagaataa acgtgatgtt atgccggcaa aaaaacacgg
     8041 caatattccc ctttagcgga tggcagagga aaatttgtat gcaaaaacct tttaagaaga
     8101 ttctcattgc aaatagggga gaaattgcga ttcgaattat tcgcgcttgt cgcgaattag
     8161 ggatcacttc agtggccgtt tttagtgatg cggatcgtga cagtttacat gttttcttgg
     8221 cagatgaggc ctatcatatt ggaccctctt cttctaaaga aagttatttg aattatcgga
     8281 aaatccttga ggtcgcgaaa accgcacatg cggatgctgt tcatccgggt tatggatttc
     8341 tttctgagaa cactatcttc gcaagagctt gtaatgaggc gggaatcaat ttcattggtc
     8401 ccactcctga aaatatcgat gccatgggag ataaaatctc ttccaagctt ttaatgcaga
     8461 aatccggcgt gccgacagtt ccgggcagcg acggcggcgt tgaaacggtg gaagaagcaa
     8521 tgtctatcgc ggaaagagtc ggacttccta ttatcatcaa ggcaaccgct ggtggtgg
//
LOCUS       DATBTE010000184         2999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1867799, whole genome shotgun sequence.
ACCESSION   DATBTE010000184 DATBTE010000000
VERSION     DATBTE010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1867799"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..716)
                     /locus_tag="VIG33_06780"
     CDS             complement(75..716)
                     /locus_tag="VIG33_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270577.1"
                     /translation="MKKWFLVVSWSVVTMACAQFQRSASSGYASRTPKVVNTYSAIET
                     PQSDATTTRIAYELGFNPANMNAEEFQQVQQRKQVRELEKSLDSRQAREQYSKVLPWL
                     KDDTEKIEMLSLPSLEGRQNWINRQNIWSRSRVPVARMKELIDSQDIAVGMPMEYVRR
                     AWGDPLSVDTSGNPIYKNERWKYMRNVPGSDGFHQQKRFVYFEGGRVVGWDTE"
     gene            823..1773
                     /locus_tag="VIG33_06785"
     CDS             823..1773
                     /locus_tag="VIG33_06785"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEY8270578.1"
                     /translation="MAQLTDPNQSKAQPIDRIGEELNPEQLENLILSEAPDFAEDLKQ
                     MKSLHVDSSVDLDVVDDGQIFSPDNPWRNASGIRKIMVTILPFLPKFWDLKQRFLMNW
                     HLHWARLSILFSQAGPRLLQATKTGALATVESSKVQISNFKKLTRSLKIVVVCLILVG
                     ALTLAFIYLSITKGVLPEEKEYLTASLEEWSAQSYQTDADTEMESFYDSTQTIQNIMS
                     LPKMIVNLQPSSLSGPNPMAAVEFFLEGLSPEAIVEVKDRETEIRDLFQRTIEEMTYT
                     EMESVEGKQLLTEKLRQILNVHLTKGKIRRVFFKEAVVKP"
     gene            complement(1770..2741)
                     /locus_tag="VIG33_06790"
     CDS             complement(1770..2741)
                     /locus_tag="VIG33_06790"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY8270579.1"
                     /translation="MSAANRVIGGLQALNDSTGATQIKRDMSAAGYILSPVNPFDDKR
                     LFLASKSRIASPYSGRISAMWEKMREDIVKIFPSDPGLQRSEKTLRLIELLYKEDSYH
                     SLSIEGYHVTMELIEKIQRGEWSPETEVKDTAQKDALAAKGYHEAFKSVVQSVTKVLQ
                     GASPGAVFYDDIQNWYRELFKPIVQAGILNPGTLAGYRKSQVYISGARHVPPPPDAVL
                     DSMETLEKLLKEESSAAVRAILGHFIFVFIHPYMDGNGRIGRFIMNLMLISGGYNWTV
                     ITTSERFQYMAALESASVNCNIVPFAHFIQSETEYWKQRSSQLVLRS"
BASE COUNT          850 a          666 c          648 g          835 t
ORIGIN      
        1 agctagagct aaagagcaat ccctcggcca tcaacagaga agggggccag tttttaaatt
       61 tcaagttgat atttttattc agtatcccag ccgacgaccc ggcccccttc aaaatagaca
      121 aaacgttttt gctgatgaaa gccatcggac ccaggaacat tgcgcatgta tttccaacgt
      181 tcatttttat agatagggtt tcctgaagta tccacggaaa gggggtctcc ccaagcgcgg
      241 cgaacatact ccatgggcat gccaacggcg atgtcttggg aatcaattaa ttctttcatc
      301 cgagccactg gaacccggga gcgactccaa atattttgac gattgatcca attttggcgc
      361 ccttccagcg agggaaggct cagcatttct attttttcag tatcgtcttt taaccaggga
      421 agaactttag aatattgctc tctggcttga cgtgagtcga gcgatttttc caactcgcgg
      481 acttgctttc gctgttgcac ttgttgaaat tcctccgcgt tcatattggc aggattaaag
      541 ccaagctcat aagcaatgcg agtggtagtg gcatcggatt gtggagtctc gatagcgcta
      601 taagtgttca cgactttcgg ggttcgcgat gcataaccgc tggaggcgga tctttggaac
      661 tgggcacagg ccatggtgac gactgaccaa cttacgacta aaaaccactt cttcatgaaa
      721 actctcctct tctttatctt aactggaggt cgactagaat agaatattct tgcgaggagc
      781 ttgcttcatt ccttacaata gtcagggaat cataaggaaa aaatggcaca actaaccgat
      841 ccaaaccagt ccaaagccca gcccattgat aggattgggg aagagttgaa tcctgaacaa
      901 ctcgagaatc ttattttatc tgaagctccg gatttcgccg aagatttaaa acagatgaag
      961 tctttgcatg tggatagtag cgtcgatttg gatgtcgttg atgatggcca aattttttcg
     1021 ccggataatc catggcggaa tgctagcggc atccgtaaaa taatggtgac gatacttcct
     1081 ttcttaccta aattttggga tttaaaacaa cgcttcttga tgaactggca tcttcactgg
     1141 gcccgtttga gtatcctttt tagtcaggca ggcccaaggc tactgcaagc gacaaagacc
     1201 ggtgcactgg cgactgtgga gtcttcgaag gtgcaaatca gcaactttaa aaaactcaca
     1261 cggagtctaa aaatcgttgt cgtttgtttg attttagttg gagccttaac cctggctttt
     1321 atctatctca gtatcaccaa aggcgttctg ccagaagaaa aggaatattt aaccgcaagt
     1381 cttgaagagt ggtccgcgca aagttaccaa accgatgccg atactgaaat ggaatctttc
     1441 tatgattcca cgcaaacaat tcaaaatatc atgtctttgc cgaagatgat agtgaacctc
     1501 cagccttcgt cgctctcggg tccaaatccc atggccgccg tggagttttt cttagagggc
     1561 ttgagccccg aagccattgt cgaagtaaaa gaccgagaaa ccgagatccg tgacctcttt
     1621 caaagaacca tcgaagagat gacctacacg gaaatggaaa gcgttgaggg caagcaactc
     1681 ctcaccgaga agctgcgcca gattctgaac gtccatctga ccaagggaaa aattcgccga
     1741 gtcttcttca aagaagcagt agtgaagcct taagagcgca aaactaattg tgagctccgt
     1801 tgtttccaat actctgtttc agactgaata aaatgggcaa atgggactat attgcaattc
     1861 actgatgccg attcgagggc cgccatatac tggaatctct ctgaggttgt aataacggtc
     1921 caattatagc ctccagaaat taacattaga ttcataatga atctgccaat gcggccatta
     1981 ccatccatat aaggatgaat aaacacgaag ataaagtgcc ctaatattgc cctcacagcc
     2041 gcagaagact cttccttaag aagcttttcc agagtttcca tagaatccaa tacggcgtca
     2101 ggaggtggag gcacgtgccg tgccccagaa atatatacct gactttttct ataacctgct
     2161 agtgtcccag gatttaatat gcccgcttgc acgatcggtt taaaaagttc tcgataccag
     2221 ttttgtatat cgtcataaaa gactgctcct ggcgatgctc cctgcaaaac tttagtaaca
     2281 ctctgaacta ctgacttaaa cgcctcatga tatcccttag ctgctaaagc atctttctgt
     2341 gcggtatcct taacttccgt ttcgggactc cactcacctc gttgaatttt ttcaattaac
     2401 tccatggtga catgatatcc ttcaattgaa agagagtgat aagaatcttc tttgtacaag
     2461 agttcgatga gtcttagagt tttttcggaa cgctgcaagc cggggtcaga cggaaatatt
     2521 ttaactatat cttctcgcat tttttcccac attgcagaaa ttctcccaga atatggagag
     2581 gctattctac ttttagatgc taaaaatagt ctcttgtcgt caaaaggatt aactggagat
     2641 aaaatatagc ccgcggcgga catatctctt ttgatctggg ttgctcctgt actatcattt
     2701 agagcttgca ggccacctat aactctattg gctgcagaca caagcttacc ttctagcaaa
     2761 actcgagata aatcagatgg gctcgcaact agcctgaggc aaatctccat atttaacgag
     2821 ttcgtagaaa aatatgttgg tgatgcctta caaattgcct cgtgaagcgg aattatatta
     2881 actccatttt tttgaacaat attagatgga aatttttttg cgtcttgata agttaagagc
     2941 gatgtatcat gcaataaatt gatggagaaa tctcaaatct agttgaaagt tgagggagc
//
LOCUS       DATBTE010000185         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1389909, whole genome shotgun sequence.
ACCESSION   DATBTE010000185 DATBTE010000000
VERSION     DATBTE010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389909"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VIG33_06795"
     CDS             <1..231
                     /locus_tag="VIG33_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270580.1"
                     /translation="KNKTYLVFVQVESPYLQVVNNRQASGLNHIAFQGVSLSHLNELE
                     KDLNKRDIKILKKDERHICFEDPNKFAVEIFI"
     gene            complement(239..1795)
                     /locus_tag="VIG33_06800"
     CDS             complement(239..1795)
                     /locus_tag="VIG33_06800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8270581.1"
                     /translation="MTALSPQDRIRQFEKRQITFSGLALLILLGISLIATSLHMQSLA
                     EQNTKFISRMIKTNDFREVGLTLHEARYDNFISIRYISKLPEHSFVLPPKLDIEKQSS
                     FWKNFFKEKVTISTDNALSSEEPDKITFEYNRFRLVPYFLVIWFILNLVSIPQTKFMK
                     KRLLEQFEKDLVVERDIAKAEIARQVRHNLRTPLAALMRIPARLPDTVKADRELLVST
                     ITQIKTLISTLDDRESAELSKNSSHDLYESLVQASREITASMPQNIRFNFDIEDSIIS
                     AMVPHIPHELRALMGNLLGNAVEALVSNGQINVTATDLGGEVQITIEDNGKGIPENIL
                     SMIFNKGFTFGKQNGTGFGLFHAKNWIESWGGHISVESVHGIKTTFTILLPIQDRAKW
                     YVPRLKFNPDSQLVILDDQETAILLWKRKIQEANLSHQTKFLAAAHEVSEIESFVQQS
                     PERFSFLFDYDLSGPVTGLDLLKSLPASSAKYLVTGNFDQAEVRAQCENEGIFLIPKS
                     QISDLTILVR"
     gene            complement(1792..>2134)
                     /locus_tag="VIG33_06805"
     CDS             complement(1792..>2134)
                     /locus_tag="VIG33_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270582.1"
                     /translation="IMEDESQPIFGFAFDKLKTTAIARSFYEAISNFAYLKDSKADFN
                     SSVNSKKDIWHLRADFTNKLSELLVPHSDSIPIQNLPEFEIQQLDISSIPDLEGCPVD
                     PVRVFAKRGTE"
BASE COUNT          662 a          414 c          435 g          623 t
ORIGIN      
        1 aaaaataaaa catatctagt ttttgttcag gtcgaatctc catacctaca ggttgttaat
       61 aaccgccagg catcaggatt gaatcatatt gcatttcaag gggtatctct atctcacctt
      121 aacgaattgg aaaaagattt aaataaaaga gacattaaaa ttttgaaaaa agatgagcgg
      181 catatttgct tcgaggaccc aaataaattt gcggtcgaaa tttttatttg aatgccattt
      241 agcgaacaag gatggtgaga tccgaaattt ggcttttagg aatcaaaaaa ataccttcgt
      301 tttcacactg agcacggact tcggcctgat caaagttgcc ggtgacaaga tatttagcac
      361 tggacgcagg caaggatttt aaaagatcta atcccgttac aggcccactc aaatcgtagt
      421 caaaaagaaa actaaatctc tccggacttt gttggacaaa tgattcgatt tctgaaacct
      481 cgtgagcggc agccaaaaac ttagtttgat gactaagatt cgcttcttgg atctttcttt
      541 tccacaaaag tattgccgtt tcctgatcat ccaaaattac aagctgtgaa tcggggttaa
      601 attttaaacg tggcacatac cacttagctc tatcttggat tggaagtaaa attgtaaaag
      661 ttgttttaat accgtgtact gactcgacgg agatgtgtcc cccccatgac tcaatccaat
      721 tttttgcatg aaaaagacca aagcctgtgc cattttgttt tccaaaggtg aatcccttat
      781 taaagatcat gctaagaata ttctcaggaa tacctttccc gttatcttca attgtgatct
      841 ggacttcgcc accaagatct gtcgcggtga catttatctg tccgttagat accaaggctt
      901 cgactgcgtt tcccagcaga ttccccatta aggcacgaag ttcatgcgga atatggggaa
      961 ccatcgcaga aataatggag tcttcaatgt caaaattaaa cctgatattc tggggcatcg
     1021 aagccgtgat ttcgcggctt gcttgaacaa gggattcata aagatcatgg gatgaattct
     1081 ttgaaagctc agcgctttct ctatcatcaa gagtcgagat gagcgtctta atttgagtga
     1141 tagtagaaac aagaagctca cgatcggcct tgacagtgtc aggcagtcta gcaggaatcc
     1201 tcatcaaggc ggcaagaggt gttctcaaat tatgacggac ttggcgtgct atttccgctt
     1261 tagcgatatc tctttcgacg acaaggtcct tttcaaactg ctcaaggagg cgctttttca
     1321 taaactttgt ttgcggaatc gatacaagat ttaatataaa ccagatgacg aggaaatagg
     1381 gcacaagtcg aaaacgattg tattcaaaag tgattttatc cggctcctca ctggaaagag
     1441 cgttgtcagt agaaatagtt actttttctt taaaaaaatt cttccaaaat gatgactgtt
     1501 tttctatatc aagcttaggc ggtagcacaa aagaatgctc cggcaattta gaaatgtagc
     1561 gaatggaaat aaaattgtca taacgagctt catgaagagt caatccaact tcacgaaagt
     1621 cattggtttt aatcattcga gaaataaatt tagtattctg ttccgctaat gactgcatat
     1681 gcaacgaagt cgcaatcaaa gaaattccta gaagaatcaa gagtgcaagg cctgagaatg
     1741 tgatttgcct tttttcaaat tgacggattc tgtcttgtgg agaaagtgca gtcactccgt
     1801 acctcgctta gcaaagactc gaacgggatc gaccggacag ccttctaaat ccggtatcga
     1861 agaaatgtct aactgctgta tttcgaattc tggtaagttt tgaataggga tactgtcaga
     1921 gtggggcacc aggagttcac ttaatttatt cgtaaaatca gctcgtaaat gccaaatatc
     1981 tttctttgag ttaacacttg aattaaaatc agcctttgaa tccttgaggt aagcaaagtt
     2041 gctgatggct tcataaaaag atcttgcaat ggcggttgtc ttcagcttat caaaagcaaa
     2101 gccaaaaatg ggctgactct catcttccat aatt
//
LOCUS       DATBTE010000186         4278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2277796, whole genome shotgun sequence.
ACCESSION   DATBTE010000186 DATBTE010000000
VERSION     DATBTE010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2277796"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VIG33_06810"
     CDS             complement(<1..270)
                     /locus_tag="VIG33_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8270583.1"
                     /translation="MKALQGKKMIVTGGSRGIGASIVKLLAEEGAQVCFTYSSREDAA
                     AEVAKSLPGEGHFYIKLDVSSEESVSQAMEQIAAKWTEVDGLVNNA"
     gene            complement(396..1367)
                     /gene="fabD"
                     /locus_tag="VIG33_06815"
     CDS             complement(396..1367)
                     /gene="fabD"
                     /locus_tag="VIG33_06815"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY8270584.1"
                     /translation="MMTLLFPGQGSQQPGMGRFLFDNFNVAKETFEEASEALSQDMKK
                     LCFEGSEAELALTENTQPALLLVSTATQRVLQCEYPLKIATAAGHSIGEYAALVAANV
                     IEFSPAMKAVRTRGQAMQSAVPVGQGGMIATLGLDTDQANFLCEYVVKTSGFAPLSPA
                     NFNSPGQIVLSGSMQAIEWLKTNFKPEIFPEPPKRAKLIPLNVSAPFHCEMMLPAEKK
                     MREVLRTMHFNIPAFKIVQNFTANFERDPEKLRENLIRQISAPVRWTESMELLKAQNF
                     THCIECGVGRVVTGLLKKIDSEAFTVLNTNSLEDLKLIEEYLKASSH"
     gene            complement(1370..2377)
                     /locus_tag="VIG33_06820"
     CDS             complement(1370..2377)
                     /locus_tag="VIG33_06820"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY8270585.1"
                     /translation="MAGTYRSRVAGTGSYLPPGILTNADLEKMVETNDQWIVERTGIK
                     RRHMASDESTTYQSTSDLSLIAAQAALGDAGLTAQDLDCIIVCTVSGDQVMPSTACVL
                     QHKLGARNVMAFDLTAACSGFVYGLSIADQFIRSGMYKHVLVLGAEVLHRLVNYQDRE
                     TCILFGDGAGAWVLSRTSVEDKNVIMSSHLHADGSLGDLFVLPAGGSKIPFSAQVLED
                     KSHYVKMKGREIFKNAVRAMSLCCQEALEANNITADEVDWLVPHQANNRIIEAIADYY
                     EFPKEKVVVSVHETGNTSAASIPLAFQMAKEEGRIKRGQMILLTAFGAGLTSGSLLLR
                     Y"
     gene            complement(2400..2588)
                     /gene="rpmF"
                     /locus_tag="VIG33_06825"
     CDS             complement(2400..2588)
                     /gene="rpmF"
                     /locus_tag="VIG33_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEY8270586.1"
                     /translation="MPTPKKKTSRSRRDMRRSHDGLSTPAIAIEKKTGELVRPHRAHK
                     ASDGAYYYKGKQISAAKA"
     gene            complement(2664..3218)
                     /locus_tag="VIG33_06830"
     CDS             complement(2664..3218)
                     /locus_tag="VIG33_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEY8270587.1"
                     /translation="MKINLSEIPEEGRTYIYTSKTGELNGVLKDLIGATPYHSEFFIK
                     PLNSKDFELSGAIETELPEECSRCGIDFCLKVKPSLNAILIPRQMDDRTGKYAKVNHI
                     SEALDDGPATAEHDGMVFDIGEFIHEAVAIAAPFNPAGPVDEKGDCSVCGITVKGRSF
                     GYDEQLPVEKPESPFAVLKNIKLN"
     gene            3430..>4278
                     /locus_tag="VIG33_06835"
     CDS             3430..>4278
                     /locus_tag="VIG33_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270588.1"
                     /translation="MIVNQKGKIGLFILAILFAACSGKPPRTQELPSTADASAELQTT
                     EKMLTEAKTNNLDILAPKSYERAEESLNDARAALTKGKSKEQVLENIAESRAWLSEAQ
                     SRGEVTRTASKGLAEARSGAIQANAPTLFSKEFGKLEKETRDLTEDAEKGDVKSIGKR
                     ADSLSQSYLKLETEAIARNTLGEAQLNLQAARKDGAAKTSPKTLAATEAKLNSALSLI
                     AQNPRNTSALNKAAAEATEQSKFLLQVNQKTKAGNTEDLVLQAEQQRRQISGLASEQA
                     QTESQLS"
BASE COUNT         1207 a         1006 c          945 g         1120 t
ORIGIN      
        1 ggcattgttc acgaggccat ctacttcggt ccactttgcg gcgatctgct ccattgcctg
       61 gctgacactc tcttcagaag agacatcgag tttgatataa aaatgccctt ctccgggaag
      121 ggatttagca acttctgccg ccgcatcttc acgggaagaa taagtaaagc aaacttgcgc
      181 accttcctca gcaaggagct tgacgatgga cgcaccaata ccgcggctgc ccccggtaac
      241 gatcattttt tttccttgca gtgctttcac attactttcc taaagcctaa agtggatcca
      301 taatttttaa tctaatttat aataaacaaa ctagaatttt ctttgttcta tttgcacaat
      361 actttgactg aaagacttgc ctacatcaag gacttttaat gactcgaagc tttcagatat
      421 tcctcaatta atttaagatc ttctaaggaa ttggtgttta gaacggtgaa ggcttcgctg
      481 tcgatttttt ttaataaacc agtcacaact ctgcccactc cgcattcaat acaatgtgtg
      541 aagttttgag ctttcaaaag ctccatgctt tcagtccagc ggacgggcgc ggaaatctga
      601 cgaatcaggt tttcacgaag tttttctgga tccctttcaa aattggcagt gaaattttgg
      661 acgattttaa atgcaggaat gttaaagtgc atggtcctca gaacttcccg catctttttc
      721 tcagctggaa gcatcatttc gcaatggaag ggagccgaca cattgagggg gatcaatttc
      781 gctctttttg gaggctcagg aaaaatctca ggcttaaaat tcgtttttaa ccactcgatc
      841 gcttgcatag aaccgctcag gacaatctgg cctggactat tgaaatttgc aggcgacaaa
      901 ggagcaaagc cggaagtttt aaccacgtac tcacagagaa aatttgcctg atccgtatca
      961 agacctaaag tcgcaatcat tcctccttga cccacaggaa ccgctgattg catggcttga
     1021 ccccgggtgc ggacggcttt catggcaggg gaaaactcaa tgacattggc tgcgacaagt
     1081 gctgcgtatt cacctatcga gtgaccagcg gccgttgcga ttttaagagg atattcacat
     1141 tgcaacactc tttgagtcgc cgtggagact aataaaagcg cgggttgggt attttcagtt
     1201 aaggccaact cagcctctga accctcaaag cagagctttt tcatgtcttg tgaaagggcc
     1261 tcactggctt cttcaaaagt ttctttagcg acattgaagt tatcaaataa aaaacgaccc
     1321 attcctggtt gttgactgcc ctgacccggg aataaaagcg tcatcataac taatacctca
     1381 aaagcaggct accgcttgtc agtcccgctc cgaatgcagt gagaagaatc atctggccac
     1441 gctttatacg accctcttcc ttcgccattt ggaaagccaa aggaatggac gccgccgaag
     1501 tatttccagt ttcatgaaca cttacgacca ccttttcttt cgggaattcg taataatcag
     1561 caatggcttc aattatacga ttatttgcct gatgcggaac taaccagtct acttcatctg
     1621 cggttatatt gttggcttcc aaagcctctt ggcagcagag actcatcgcg cgaaccgcat
     1681 ttttaaagat ttcacgcccc ttcattttta cataatggct tttatcttcg agaacttgtg
     1741 cggagaaagg aatcttggat cctcccgctg gcaatacgaa gagatctcca agggagccat
     1801 ccgcatgcaa atgagagctc atgatgacgt ttttatcttc aacagaagtc cgagaaagaa
     1861 cccaagcccc cgcgccatca ccaaaaagga tgcaagtttc gcggtcctga taattgacca
     1921 gacgatgaag aacttcggct cctaagacga gcacatgttt gtacatgccg ctacgaatga
     1981 actgatcggc tatggacaat ccatagacaa aacctgagca ggcagccgta agatcaaagg
     2041 ccattacatt tcgagctcca agcttgtgct gaaggacgca ggccgtgcta ggcataacct
     2101 ggtcgccaga aactgtacaa acgataatac agtcaagatc ttgcgcagta agccccgcat
     2161 cgccgagagc tgcctgggca gcgataagac tcaagtccga agtggattga tacgttgttg
     2221 actcatcgga ggccatatgg cgacgtttaa ttccggtgcg ctcaacgatc cattgatcgt
     2281 tggtctcaac cattttttcc agatcggcat tggttaagat gcctgggggc aaataggaac
     2341 ctgttcctgc tacacgagat ctatatgttc cagccataga ttaatcctct tcctaagaat
     2401 taagctttag cggcgctaat ttgtttacct ttgtagtagt aagctccatc agaagccttg
     2461 tgagcacggt gagggcgtac aagctctccc gtttttttct caatggcaat ggccggagtg
     2521 ctcaaaccat catgggatct gcgcatatcg cgacgtgaac gggatgtttt ctttttagga
     2581 gttggcatag ttttttacct cttaagctat ctctatcagt tacaaagctc gttaatttaa
     2641 ccaaagatca ataaaaatca acattaattc aacttaatgt tcttaagaac cgcaaaaggg
     2701 gactctggct tctcaacggg aagctgttca tcgtagccaa aactacgtcc cttcacggta
     2761 attccgcaga cggaacagtc gcctttttca tccacaggcc ctgccggatt aaaaggcgcg
     2821 gcaatggcca cggcttcatg gataaattca ccaatatcga aaaccattcc gtcgtgctca
     2881 gctgttgctg gtccatcgtc aagtgcttca gaaatgtgat ttactttagc gtattttcca
     2941 gtgcgatcat ccatctggcg cgggataaga atggcattca gggaaggctt caccttaaga
     3001 cagaaatcga tgccacaacg ggagcactct tccggaagtt cagtttcgat agctccagac
     3061 aattcaaaat ctttggagtt taagggcttg ataaaaaatt cactatgata aggggtggcg
     3121 ccaataaggt cctttaaaac tccattcagt tccccggttt tactggtata aatgtaggtt
     3181 cttccctctt ctgggatttc agacagattg attttcatag accctccgca agactcaaaa
     3241 aagaggctca acttagccgg acagcgtcaa caatgcaagt ggtgaattga cgaattactg
     3301 caaaaaataa gccgaatgta atttaccatg aacaaacttc tacctttgta atattttaag
     3361 ctccgtttct aaataaagag tgatttctcc tatggaggac tttttaaatt ttttagaaaa
     3421 ggagttttta tgattgtcaa ccaaaaaggt aaaattggtt tattcattct agccatcttg
     3481 ttcgcggcct gttctgggaa accaccaagg acgcaagagc taccctcaac tgcggacgct
     3541 tcggctgaac tgcagaccac tgaaaaaatg ctgactgaag ctaaaaccaa taatcttgat
     3601 atattggcgc cgaaaagcta cgagcgtgct gaggaaagtt tgaatgatgc tcgcgcagcc
     3661 ttgaccaaag gaaagagtaa agagcaggtc ttggaaaaca ttgctgaatc ccgcgcctgg
     3721 ttgtccgaag ctcaatctcg tggagaagtc actcgtacgg cgtccaaagg cttggcagag
     3781 gcgagaagcg gcgcgattca ggccaatgct ccgactctct tttcgaagga gtttggcaag
     3841 ctagaaaaag aaacgcgtga tttaaccgaa gacgctgaaa aaggagatgt taaaagtata
     3901 ggaaaaaggg cggactccct gtctcaatct tatcttaagc ttgaaactga ggctatcgct
     3961 agaaacactc tcggcgaagc tcaactcaat ttgcaagcgg ccagaaagga tggggcggct
     4021 aaaacttctc ctaaaacttt agcggccact gaagctaagc tgaactcagc cctttctttg
     4081 attgcgcaaa atccacgcaa caccagtgcg cttaataaag ccgccgctga ggcaacagaa
     4141 caatccaagt tcttactcca agtgaatcag aagaccaagg cgggaaatac ggaagacctg
     4201 gtcttacagg cggaacaaca aagacgccag atctccggtt tggcctctga gcaggcacaa
     4261 acagaatctc agttgagc
//
LOCUS       DATBTE010000187         2740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_433976, whole genome shotgun sequence.
ACCESSION   DATBTE010000187 DATBTE010000000
VERSION     DATBTE010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433976"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="VIG33_06840"
     CDS             complement(<1..1179)
                     /locus_tag="VIG33_06840"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8270589.1"
                     /translation="MSKEISYSDGEIENEILQTLKESSNVSAAATIAIEKQTSWAFEY
                     HLSCQRANLVRHFNFQGKRVLEIGAGMGAVSRYVAEKCEFLQIVEGTSRRLSCLQERL
                     RDLRNFNVFCGNFKDFKVEEKFDVILIIGVLEYADLYFEDDGEDPFVAFLKKAKSHLK
                     PEGEVLLAIENRLGLKYWAGATEDHTGAFFDGILGYRNAKTVRTFSKKEIRTVLESAG
                     FLEVNFQYPFPDYKLPRSILTEELANRCPDVFASIAGASLARDYGRDRVVLFSESVGF
                     NGLIDTGLLTEFSNSFLIISRQNKILKKDKVLGYSYSQNELKKTMTHFEEINDSLMVN
                     KRLMDSDADEVDYQTIRWIRPQATEVKRGALLIDRFKRSLTFREWEIFFEEWKKYVSW
                     I"
     gene            complement(1163..2431)
                     /locus_tag="VIG33_06845"
     CDS             complement(1163..2431)
                     /locus_tag="VIG33_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8270590.1"
                     /translation="MNDIAIQVKNLTKTYSLYEKPIDRLKEALHPWRKSYNREFKALN
                     DVSFEIKKGQTVGIIGKNGSGKSTLLKIIAGVLTPTSGEAQVKGSVSALLELGAGFNP
                     ELTGFENIYLNGNLMGFSKNEMDARLSEILDFADIGDFVHQPVKMYSSGMFIRLAFAV
                     AIAVEPEILIVDEALSVGDIFFQSKCYAYFEKLKEQGKTILFVTHSLDAVIRNCSRAF
                     CIEEGKITASGAPSEVVDFYKKQRTGHVHKENVAIELGQKWSNSYILNNSCDSYGSHE
                     VFIEDFAILDCHGIPQSVFFQGDMVELALKVKVNKPIRKLIGAFAIKTPDGLEVLGSN
                     TAIMKRDLHEIVEAEQGEIRFKFPLFLQSSEYTLSLGLTHIDECGELHVYHRMYNVVS
                     LVVNSVFGGTGIYTPSINLAVNIGVNEQRN"
     gene            complement(2434..>2740)
                     /locus_tag="VIG33_06850"
     CDS             complement(2434..>2740)
                     /locus_tag="VIG33_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8270591.1"
                     /translation="TSAVVVFYRDLGQVIGMLIQIGFWITPIFWSPETLPEEFKFLFF
                     FNPVSYVVMGYRNSLISKIWFWQEPWQTFYFWLLVFSLSIFGLFIFKRLRPHFADVL"
BASE COUNT          863 a          679 c          455 g          743 t
ORIGIN      
        1 aatccacgac acatattttt tccactcctc aaagaaaatc tcccactctc taaaagtaag
       61 ggatctctta aacctgtcaa taagaagagc cccccttttc acttcggtcg cctgaggacg
      121 gatccacctt attgtctgat agtccacttc atcagcatcg gaatccataa gcctcttatt
      181 caccatcaag gaatcgttaa tctcctcgaa atgagtcatt gttttcttaa gttcattttg
      241 tgaataacta tatccgagga ctttatcttt ttttaaaatt ttattttgcc tagaaattat
      301 cagaaaagaa ttcgaaaact cagtcaataa accagtgtca ataagcccat taaatcccac
      361 ggattcagaa aataatacga ctctatcccg tccgtaatct cgagctaatg aagccccggc
      421 gatactggcg aaaacatccg ggcaacgatt tgccaactct tcagttaaaa tgctgcgtgg
      481 aagcttatag tcaggaaagg gatattgaaa attgacctct aaaaatcccg cactttccag
      541 aacagtgcgg atttcttttt tactaaaggt tcggacggtt ttcgcatttc tataacccaa
      601 aatgccatca aagaaagcac cggtgtgatc ttccgtcgca cctgcccaat actttaaacc
      661 caatcgattt tctattgcta aaagaacttc cccttcgggc tttaaatggc tcttggcttt
      721 tttcaaaaaa gcgacaaagg gatcttcacc atcatcttca aaatagagat cagcatactc
      781 aagcacacca ataatcaaaa taacatcaaa cttttcttca actttgaaat ccttgaaatt
      841 cccacaaaaa acattaaaat tacgaagatc ccttaaacgc tcttgaagac aagagaggcg
      901 ccgggaggtt ccctctacaa tctgtagaaa ctcgcacttt tccgccacat atcttgaaac
      961 cgcccccatt cccgcaccaa tttcaagaac acgcttgcct tgaaaattga agtgcctgac
     1021 caggttggcc ctttgacaag aaagatgata ttcaaaagcc caggaggttt gcttttcaat
     1081 ggcaatggtc gctgccgccg aaacattcga cgattccttt aacgtctgca atatctcatt
     1141 ttcaatttcc ccatcagaat aactaatttc tttgctcatt tacaccaatg tttaccgcaa
     1201 gattgatact gggcgtatag atccctgtac cgccgaagac actattaacc accaaactca
     1261 caacattata cattcggtga taaacatgaa gctctccaca ttcatcaatg tgtgtaagac
     1321 ctagcgacaa tgtatattcc gaactctgca aaaataaggg gaatttaaat cttatctcac
     1381 cttgctcggc ttcaactatc tcatgcaggt cccgtttcat gatcgctgta ttagatccca
     1441 aaacctctaa cccatcagga gtcttgatcg caaatgcgcc aatcaatttt ctgatgggct
     1501 tattcacttt tactttcaag gccaactcaa ccatatctcc ctgaaagaag acactctggg
     1561 gaataccatg gcaatccaaa atagcgaaat cttcaataaa aacttcatga gacccatagg
     1621 aatcacagga attatttaga atataagaat ttgaccattt ttgccctaac tcaatcgcga
     1681 cattctcttt atgcacatga cctgttcgct gctttttgta aaagtccaca acttcagagg
     1741 gagcccctga ggcggtaatt tttccttctt caatgcaaaa ggcgcgggaa caattacgta
     1801 taaccgcgtc caaactatga gtgacaaaca aaatcgtctt gccctgctct tttagctttt
     1861 caaaatacgc atagcacttg gattgaaaaa aaatatctcc gacactcaaa gcctcatcta
     1921 cgatcaaaat ctcaggctcc acggcgatcg cgacggcaaa agccagccga ataaacatcc
     1981 cactcgaata catcttcacg ggttgatgaa caaaatcccc gatatccgcg aaatcaagaa
     2041 tttcagaaag acgcgcgtcc atctcgtttt tggagaaacc cataaggttt ccgtttaagt
     2101 atatattttc aaatcccgtg agttcgggat taaagccggc acctagctca agcaaggcgg
     2161 agacgctccc cttcacttga gcctcacccg aagtcggcgt taaaactccg gcaatgatct
     2221 tcaataaggt cgatttgcca ctgccattct ttccgataat gccgacggtc tgaccttttt
     2281 ttatctcaaa ggacacatcg ttgagagcct taaattccct attgtagctc ttgcgccaag
     2341 gatgcaaagc ttctttcaaa cggtctatgg gtttctcgta aaggctgtat gttttggtaa
     2401 gatttttgac ttgaattgca atatcattca tagctacaac acatccgcaa aatgaggtct
     2461 cagtctctta aaaataaaaa gcccaaaaat actcagacta aaaacaagga gccaaaagta
     2521 aaaggtttgc caaggctcct gccaaaacca aattttagaa attaaactat ttctataccc
     2581 cataaccaca tagctaacag gattgaaaaa aaagagaaat ttaaattcct ctggaagagt
     2641 ttccggtgac cagaaaatcg gcgtaatcca aaatcctatc tgaattaaca tgccaataac
     2701 ctgccccagg tcccgataga aaaccaccac ggcgctcgtt
//
LOCUS       DATBTE010000188         8741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1595168, whole genome shotgun sequence.
ACCESSION   DATBTE010000188 DATBTE010000000
VERSION     DATBTE010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 8741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8741
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595168"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VIG33_06855"
     CDS             complement(<1..1075)
                     /locus_tag="VIG33_06855"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8270592.1"
                     /translation="MAKKLEKYIAHQIVTEWARIAEQTLAEVQRREVSIEFSKTGAIA
                     LIGVRRCGKTSLALLKAKEINATNYLYINFEDPRFIEYRDYSVFDILIEAYVENQKTY
                     PGVIVFDELQNISGWERWVRKFVDSKKTKLIITGSSAKLLSGELATAVAGRVLDYRIW
                     PLSFKEFVKFKEKSGSQESLSLLPEYIEFGGFPEVILTSQVELKKKILKQYFTDILFK
                     DVMSRKGIRDRHTLDKIIYHYLTNISCHHSYSSLKKAYQLGGDTPHNYTSYLEDAFLI
                     FEVERFHKNLKVQSRDTKKVYAIDTGLRNLNSKSLTEDFGKLLENIIFLELLRTGNQI
                     YYFKETLETDFVVVEGNKAKRAIQ"
     gene            complement(1303..1539)
                     /locus_tag="VIG33_06860"
     CDS             complement(1303..1539)
                     /locus_tag="VIG33_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270593.1"
                     /translation="MSFKFNITELGAREPTWLETDEEQDLHYSSAVTLSRDDAFKIRE
                     ILLSAIKNSVDHIVKSPEEDVFALIVDFIGLSQK"
     gene            complement(1669..2919)
                     /gene="epmA"
                     /locus_tag="VIG33_06865"
     CDS             complement(1669..2919)
                     /gene="epmA"
                     /locus_tag="VIG33_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HEY8270594.1"
                     /translation="MTRHEFLEQKWTRYPVLPEKISFVGRIHEIQFRGTCWEVTVTRE
                     GKSYNQLFPKLFFREIPNLNVAKILLSGDLVGISEEGLVTLLAPSLQQLPARALRLET
                     LRLWQDYVYWLREFFRQQGFVEIATPSLVKCPGTEPSLDVFSTEFINGSRKEKLYLPT
                     SPELHLKKALSLGAEKIFEIKNVFRNGEVTDHHQPEFTMIEWYRAYVDLQSIKADVTS
                     LVNYLVQKIPAFKNSLGPSAGFKSFTVQQLFLQHCGFDFKPNTSAEQLKALAEKLKVD
                     VRSATSIDDYFFLLFMEKIETQFAPECVTFVEKYPPYQAALARISTDGWGDRFEVYWQ
                     GLELANAFHELNDPMAQKQRFAEDIAKKRSLKKEEVPLDSEFMQCLEAGMPPSAGIAL
                     GVERLFMALTGIKNISEIRLFPMK"
     gene            complement(2920..3309)
                     /locus_tag="VIG33_06870"
     CDS             complement(2920..3309)
                     /locus_tag="VIG33_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270595.1"
                     /translation="MKSLIAMTFIILGINLWSTLSVASPQACKYISEGAVQQSWKKIR
                     IQILNGVILTGADSFSEAINLGKSLLDGQICFPHLQDCRLASEGIVGGVIMKQRIQMD
                     GEMVFGAEDMATALNELSQLRSLGFCK"
     gene            3437..4894
                     /locus_tag="VIG33_06875"
     CDS             3437..4894
                     /locus_tag="VIG33_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY8270596.1"
                     /translation="MSQALITVDFSKSLSDFKTQVDWVGLRYYSEKTTRHLIRNENPE
                     SHSSQQDQGVMLEVMIDGHLGYAATSDFTPEGLKTAFQKAKLLSTQAARFKAAEFSQE
                     QRPASRGNFQSPYLHRLDTLATEEIISVLMKASQSLKVSDKIVARTAHAMLIETEIQY
                     LSSSGAEWIQNFNMVNTSYAATAQDNKDAQTRSFGNSVQIGAEAFRETSLMKPTISEL
                     CEMTGTEAMELLEAENCPTETMDLLLAPDQMQLQIHESIGHPLELDRILGDERNYAGW
                     SFIKPENFGRLQYGSPLLNVIFDPQEKGQLASYAFDDTGNPATPEYLIKNGILLSGLG
                     GLESQQRLRIPGVANQRSSSWNRAPIDRMANINIEPGTDTLEQMIASVKRGILMKTNR
                     SWSIDDYRNKFQFGCEYGQLIEDGKLTRTVKNPNYRGKTLQFWSSLKAVGNAKTYESH
                     GTPYCSKGEPNQVIRVAHASPYCLFANIEVFGGLS"
     gene            4891..6225
                     /locus_tag="VIG33_06880"
     CDS             4891..6225
                     /locus_tag="VIG33_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY8270597.1"
                     /translation="MSQLNQREIFNFVAQALMNELHQDETLTMDFRGEETDFIRWTQS
                     KVRQNTSVYQKAISLTLHKAGRKLQKSFSSQNSKEWDLLEARKLLQEARQEAPALPVD
                     SYLVEFTNHGKSEAHFQGSLLKGEDLITAIAHPCASFDMAGLYAGGSIEVGNCNSQGQ
                     NHWFVMENFFMDYSLYNGERAIKSCYAGFKWNQKEFESKLGLASEQLRAMNQPRKKLE
                     PGKYKVYLAPAAVGELASMFSWGALSLRAYKTGSNALKKLRDGERTLSSKLSIAENFH
                     LGLTPRFNDLGELAAERISLIKEGQFEQFLVSSRSAKEYSVPGNHANSDEVPRAFEIA
                     PGILKKENILKELGTGLYLSNLHYLNWSDLQNARITGMTRYACLWVENGEIIAPIEDM
                     RFDVSLFDILGEGLLEVTDFLEVDPSVETYFQRALGGKNLPGMLMENFSFTL"
     gene            6337..6696
                     /locus_tag="VIG33_06885"
     CDS             6337..6696
                     /locus_tag="VIG33_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270598.1"
                     /translation="MEGKIQQLVNELSKNERVQSVIHDVHVLQDKLNAEMNKTISKFK
                     KSAGNIEKNILAYKKQLLTQKTKLQKELQAKAKSLKAKPFAKQAAAVVKTTTKKASTP
                     AKRKTTVARKATTKKKA"
     gene            7054..7431
                     /locus_tag="VIG33_06890"
     CDS             7054..7431
                     /locus_tag="VIG33_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270599.1"
                     /translation="MKILFITLLYSSSVALAQASTKAVVHKQNEQMTQQFRNNQMIQM
                     QMELSKVETELNRKNQEKTELKSQLADMKSPQKMAKTLKLNSKVKQFDKAEAEIQNHL
                     QQLEIDIKDLKQKQASLTENLKN"
     gene            7474..>8741
                     /locus_tag="VIG33_06895"
     CDS             7474..>8741
                     /locus_tag="VIG33_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270600.1"
                     /translation="MNKFILKTVLMTSLLGMGIIGCTSKSSDTPSAGIASITVTGAIA
                     SATSMSVDANVGKMALTLGNLEIYVIYTSGTTVGITQASVGTDGAWSFSVPSGAQINA
                     IVRDKTSLELVGPIVFVDDSNTDLDGNAKTSTVTSLKSGASMGTITLSSEGKFEVPVA
                     QIAAAQDTVVAAPTSKIDFTGAWTIGKYDGTLPDSTYKTAVAVGDAGCPTEQNCQGTP
                     VGQQVYMLKLSGKKFTYPSTGLCATYKASHTGSCNPDTDGTTDATTTIDAASIWGGAA
                     AIAGCGYKLGFTRDDVAAGGGMNLAAADLPTVNTTQLTFGGVTWSSFPTGWNQVVGGV
                     TYPWANASATTNYPMMNCTQVDKTVSSTTYKINVCKGKLQSDHTTVKYQANYGGGCLN
                     ADGKPVVIKDWSALGTPASCNGPTNFAVTG"
BASE COUNT         2638 a         1967 c         1815 g         2321 t
ORIGIN      
        1 cctgtatcgc ccgttttgcc ttattcccct cgacaactac gaaatcagtc tcaagggttt
       61 ctttgaagta ataaatctga ttgcctgttc ttaacaactc caaaaagata atattctcta
      121 aaagcttacc aaagtcttca gtaaggcttt ttgaattcaa gtttctgagt ccggtatcaa
      181 tagcatatac tttttttgta tccctggatt gaaccttaag atttttgtga aagcgctcca
      241 cttcaaaaat cagaaaagca tcctcaagat aacttgtata gttatgaggc gtatcccctc
      301 caagttgata agcttttttc agagaagaat aggagtgatg acaggaaata ttggtcaagt
      361 aatgatagat gattttatca agagtatgtc tatctcgaat acccttacga ctcataacgt
      421 ctttaaataa aatatcggta aagtactgct taagtatctt tttcttcagc tcgacttgcg
      481 aagtgagaat aacttccgga aatccaccaa attcaatata ttccggaagt aaactcaagg
      541 attcttgaga tccacttttt tccttaaatt ttacgaattc cttaaagctc aaaggccaaa
      601 tacgataatc caaaactcgg ccggccacgg ctgttgccaa ctcaccactc aataattttg
      661 ctgaagaacc agtgataata agtttagttt ttttactgtc gacgaactta cgaacccaac
      721 gttcccatcc cgaaatattc tgaagttcat cgaagacaat cactccgggg tacgtctttt
      781 gattttcgac gtatgcttcg atcaaaatat caaatacact ataatctcgg tactcgataa
      841 agcgagggtc ttcaaaatta atatacaaat aattagtggc gtttatttcc ttagctttta
      901 aaagcgctaa tgatgtttta ccacaacgac gaactccaat aagcgcgata gcccctgttt
      961 ttgaaaactc tattgagact tcccgccttt gaacctctgc cagagtttgc tccgcaatcc
     1021 gagcccattc ggtaacaatt tgatgagcaa tgtatttctc aagttttttg gccatgctta
     1081 attttagacc gtatgcggtc taaatatcaa gcaactttag actgcatacg gtttaaagtt
     1141 gaatttacta ataatatcat aaatatagtg cgtattttaa ccctactttc atttcctgct
     1201 acgccaaaat tattgcatag gcgactgctc aggcaaaatt tcgcgtccct taagaatctc
     1261 agtttggcta tccagaggat agcccattgc cgaaatgaag cattatttct gacttagccc
     1321 tataaaatca actatcaggg caaagacatc ttcctcagga gatttaacga tatgatccac
     1381 cgaattcttg atggccgaaa gcaaaatctc acgaattttg aaggcatcat cacgtgaaag
     1441 agttactgcc gaggaatagt gcaaatcttg ctcttcatcg gtttccagcc atgtaggttc
     1501 tctggcccct aattccgtta tattgaattt aaaagacacg tcattgaaag ggctacagtc
     1561 acatcgcttg ccttagaact ccacgaatct tgaagaaaag cagactgatt ttaaaggtcg
     1621 cattcttgag caaaggaacc agacagctcg atctatgcat aatcattttt acttcatcgg
     1681 aaacagccgg atctcagaaa tattcttaat ccccgtcaag gccatgaaga gtctctcgac
     1741 cccgagagcg attcccgctg aaggcggcat tcccgcttcc aaacattgca tgaattcaga
     1801 atccagcggg acctcttctt ttttgagaga tcttttctta gcaatgtcct ccgcaaatct
     1861 ttgtttttga gccatgggat cattgagttc gtgaaaagcg ttggcgagtt ctaaaccctg
     1921 ccaataaact tcgaaacgat cgccccaccc gtcagtactt attcttgcaa gagccgcctg
     1981 gtaaggagga tatttttcca cgaaagtcac gcactctggc gcgaactgag tttcgatttt
     2041 ctccatgaag agcaggaaaa agtaatcgtc aatgctagtg gccgatctca catccacttt
     2101 caatttttcg gccagagctt ttaactgctc cgcactggta ttgggtttaa aatcaaaccc
     2161 acaatgctga agaaaaagtt gttgaactgt aaagctctta aaccctgcgg aaggccccag
     2221 ggaattcttg aaggcaggaa ttttctgaac cagataattt accagactcg tcacatcggc
     2281 cttgatgctt tgcaggtcca cgtaggctcg ataccactcg atcatggtaa attcgggctg
     2341 atggtggtcc gtgacttctc cgttacgaaa aacattttta atttcgaaaa tcttttccgc
     2401 ccccagagac aaagcctttt tcagatgaag ttcaggactg gtgggtaaat agagcttttc
     2461 tttgcgcgaa ccattgatga actccgtact aaacacatcc agacttggct cagtgcctgg
     2521 acattttact agtgaaggtg ttgcaatctc aacgaagcct tgctggcgaa aaaattctct
     2581 caaccaataa acataatctt gccacagtcg gagggtctct aagcgcaatg ctcgagccgg
     2641 aagctgttgc agcgaaggcg ccaacagcgt cactaggccc tcttcggaga tccctacgag
     2701 gtcgccgcta agaaggattt ttgcgacatt caaattcgga atttcacgaa aaaataattt
     2761 tgggaagagt tgattgtagc tttttccctc gcgcgtgacc gtcacctccc agcaggttcc
     2821 ccgaaattga atttcatgaa tacgtcccac aaaagatatc ttttcgggaa gaacagggta
     2881 gcgagtccac ttttgctcta aaaattcatg gcgagtcatt tatttgcaaa aacccaaact
     2941 gcgcagttga gacaactcat taagagccgt agccatatcc tcggcgccga aaaccatttc
     3001 gccgtccatt tggattcgct gcttcatgat gactccccct acaatgcctt cgctcgccaa
     3061 acggcaatct tgaaggtgag gaaaacaaat ttgcccatcc agcaaacttt tccctaggtt
     3121 aatggcttca gaaaaactat cagcccccgt caagataaca ccattgagaa tttgaattcg
     3181 aatttttttc caagattgct gaacagcacc ttcagaaata tatttacaag cttgtggaga
     3241 agccactgat aaggtagacc acaggttgat gcccagaatt ataaaagtca ttgcaatgag
     3301 tgatttcata gatctctaag tttgccactt ccggctgcca acggctagag accttaagtc
     3361 tagagagggc tcaatgaacc aggggatggt ctagtgatta agggcactcc ctactcactt
     3421 aattatagga agcaaaatgt ctcaagctct tattaccgtc gatttttcca agtccctttc
     3481 tgacttcaaa acgcaagtcg attgggtcgg tctaagatat tatagcgaaa aaacaactcg
     3541 ccacttgatt cgtaacgaga accccgaatc ccactcctcg caacaggacc agggtgtcat
     3601 gttggaagtc atgatcgatg gtcaccttgg ctatgccgcc accagcgact ttacgcctga
     3661 agggttgaag acggctttcc aaaaagcgaa actgctttca acccaggccg cacgctttaa
     3721 agccgcagaa ttctcacagg agcagcgtcc ggcctcccgc ggaaattttc aaagtcctta
     3781 tctgcaccgc ctggatactt tggctacgga agaaattatc agtgtgctga tgaaggcttc
     3841 tcagtcgctg aaggtttcgg ataaaattgt ggctcgaacc gcccatgcga tgcttattga
     3901 aactgaaatc cagtatctct cctccagtgg agcggagtgg atacaaaatt tcaatatggt
     3961 caacacctct tacgccgcga ccgcccaaga caacaaagat gctcagacgc gctcttttgg
     4021 aaattctgtc caaataggag ccgaggcttt ccgtgagacg agtctcatga aaccaacgat
     4081 ctcagaactt tgcgaaatga ccggaactga agcgatggag ctgcttgaag cagaaaattg
     4141 tccgacggag acaatggatc tcttactggc tcccgatcaa atgcaactgc aaattcacga
     4201 atccatcgga catcctttgg aattggatcg tattcttggc gatgaaagaa attatgccgg
     4261 ttggagtttt atcaaacccg aaaatttcgg aagactgcaa tacggctccc ctcttttgaa
     4321 tgtgattttt gatccccaag aaaaaggaca actggcctcc tatgcctttg atgataccgg
     4381 caatccggcc acccctgagt acttgatcaa aaacgggatt cttttgagtg gactcggcgg
     4441 cctcgaatcc cagcaacgcc ttcgtatacc cggggttgct aaccagcgtt cttccagttg
     4501 gaatcgcgcg cccatcgatc gcatggcaaa tatcaacatt gaacctggca cagacaccct
     4561 cgaacaaatg attgcttcgg taaaacgcgg aatccttatg aaaaccaatc gttcttggtc
     4621 gattgatgat tatcgcaaca agtttcaatt tggttgtgaa tatggccaat tgatcgaaga
     4681 cggaaagctc acccgcactg ttaaaaatcc caattatcga ggtaaaacac tgcagttctg
     4741 gagcagtctg aaagccgtcg gcaatgccaa aacttatgaa agccatggca caccttactg
     4801 tagcaaaggg gaaccgaacc aagtcattcg cgtggctcat gcttcaccct attgtctttt
     4861 tgccaatatc gaagttttcg gaggtctctc atgagtcagc taaatcaacg cgaaatcttt
     4921 aattttgttg ctcaagcttt aatgaatgaa ctccatcaag atgagacttt gacaatggat
     4981 ttccgtggcg aagagactga ttttattcgt tggacccaga gcaaagtccg tcagaatact
     5041 tccgtctacc agaaagcgat cagcttgacc ctgcacaaag caggccgcaa actgcaaaaa
     5101 tcttttagca gccaaaactc aaaagagtgg gacctccttg aagcgcgcaa gcttttacaa
     5161 gaagcccgcc aagaagctcc cgcacttcct gtggattctt atttagtgga gttcaccaat
     5221 catggaaaaa gcgaggccca tttccaggga agcttgctca aaggcgaaga cctcatcacc
     5281 gccattgctc acccctgtgc ctcctttgat atggcgggac tttatgcagg gggcagtatt
     5341 gaagtgggaa attgcaactc ccaaggacaa aatcattggt ttgtaatgga aaactttttt
     5401 atggactatt cactctataa tggcgaacgt gccataaagt cttgttatgc gggatttaaa
     5461 tggaatcaaa aagagttcga atccaagtta ggacttgcca gcgagcaact gagggccatg
     5521 aatcaacctc gtaaaaagct agaacccggc aaatacaaag tttatttagc accggcagcg
     5581 gtgggtgaat tagccagcat gttttcctgg ggagcactga gccttcgcgc ctataaaact
     5641 ggaagtaatg ctctaaaaaa actccgggat ggcgagcgca cgctgtcttc aaaactttcc
     5701 atcgcggaaa acttccactt gggcctgact ccgcgcttta atgacctagg ggagcttgct
     5761 gcagagagaa tcagccttat caaggaagga cagttcgaac aattcttggt cagttcacga
     5821 agcgccaaag aatactcagt ccccggtaat cacgcaaata gcgatgaggt ccctcgagcc
     5881 ttcgaaatcg ctccaggcat ccttaaaaaa gagaacattc tcaaagagct tggaacaggg
     5941 ctctatcttt cgaatttgca ttacctgaac tggagtgatt tgcaaaatgc ccgcatcacc
     6001 gggatgaccc gctacgcctg tctttgggtt gaaaatggag aaataatcgc tcctatcgaa
     6061 gatatgcgat ttgacgtcag tcttttcgat attctggggg aaggattact cgaagtgacc
     6121 gactttctgg aagttgatcc gagtgtggag acctacttcc aacgagccct aggcggaaaa
     6181 aatctgccgg ggatgttgat ggaaaacttt agctttacgc tttaaagcgc caaaaatgac
     6241 tctttcgtaa aattattgca gggggcttcc aatttcctga agacgggggt ataaacaatc
     6301 cgtcgatcta aaattcttct caaggagcgt aaaaagatgg aaggtaaaat ccaacaactc
     6361 gttaacgaac tttctaaaaa cgaacgtgta caatctgtaa ttcatgatgt tcatgtatta
     6421 caagataaac tgaatgcgga aatgaacaag actatttcta aattcaaaaa gtccgcaggc
     6481 aatatcgaaa aaaacattct agcttataaa aagcaattat tgacccaaaa gacaaaattg
     6541 caaaaagagc ttcaagcaaa agcaaagtct ttaaaagcaa aaccttttgc caagcaagct
     6601 gcagcggttg taaaaacaac aactaagaaa gcttcaacac cagctaaaag aaaaaccact
     6661 gttgctcgta aagcgactac caagaaaaaa gcataattat taattgagta ataaatctag
     6721 aaagcccgtg tctgaaaaga tgcgggcttt ttttataatt atttaaaatt tctgaagcta
     6781 ttattctaat tttagaatcc tactagacta gaccattgcc acccttattt agcagcccta
     6841 ccagtcaatt catttgaact gtgatcaagt aaaaaaatta actgtatcaa aatcatttat
     6901 taaagtctcg attcgcaaca cggaaccatc gttggtcctg attcttcttc ttacaaacaa
     6961 ttattttgaa ggacttcaaa aactccttca gaattttggg ggggaatccg aaataccctc
     7021 tagccagtaa ttagaccaac atcgaggaac gttatgaaga tactttttat aacattacta
     7081 tatagttcat cagttgcctt agcccaagct tcaactaagg ctgttgttca taaacaaaat
     7141 gagcaaatga cccaacagtt tcgcaataat caaatgattc agatgcaaat ggaattgtct
     7201 aaagtagaaa ctgaattgaa ccgaaaaaat caggagaaaa ctgaactaaa aagtcagctg
     7261 gctgacatga agtcaccgca aaagatggct aagactttaa aattgaattc aaaagtaaag
     7321 caattcgata aggccgaggc ggaaatacaa aaccaccttc agcaattgga aattgacatt
     7381 aaagatttaa aacaaaaaca ggcttctctt actgagaatc ttaaaaattg aacttaaata
     7441 ttgaactatg aaccaaacaa aggagtgttt tagatgaata aatttatact gaagacagtc
     7501 ttaatgacct ctcttctagg gatgggcatt atcggatgta caagtaaaag cagcgatacc
     7561 ccctctgctg gaatcgcaag tatcacagtc acgggtgcaa tcgcttccgc gaccagcatg
     7621 tctgttgatg cgaatgtggg caagatggct ttaacgcttg gaaatctgga aatctatgtg
     7681 atttacacta gtggaaccac tgtaggtata acacaggcct ccgttggaac tgacggcgct
     7741 tggagtttta gtgttccatc tggagcgcaa attaacgcga tcgtcagaga taaaacaagt
     7801 cttgaacttg tcggacctat cgtatttgtg gacgacagca atacagatct tgatggaaat
     7861 gctaagacct ctactgtaac atctttaaaa tccggtgcct caatgggcac aatcactttg
     7921 agctctgaag gaaagtttga agttcctgtt gctcaaattg cggcggcaca ggatacagtt
     7981 gttgcggctc ctacttctaa gatagacttc actggtgcgt ggactatcgg taaatacgac
     8041 ggcacattac ctgacagcac ttataaaaca gctgtagctg tcggtgatgc tggctgtcct
     8101 accgaacaaa actgccaagg aactccagtt gggcaacaag tttacatgct taaactgagc
     8161 ggtaagaaat tcacttatcc ttctactgga ctttgcgcga cttataaggc tagccatact
     8221 ggatcttgta atccagatac tgacggaacg actgatgcaa caacgactat tgatgcagcc
     8281 tcaatttggg gtggagctgc cgcgatcgct ggttgtggtt ataagttggg tttcacaagg
     8341 gatgacgttg cggctggcgg tggaatgaat ctggctgctg ctgacttgcc gacagtaaat
     8401 accactcaat tgacttttgg tggtgtaact tggagcagct tccctactgg ttggaatcaa
     8461 gttgtaggtg gagtaactta tccatgggcg aatgcaagtg ccacaactaa ttatccaatg
     8521 atgaattgca ctcaagttga caagactgtg agttcaacaa cttataaaat caacgtttgc
     8581 aaaggaaaac ttcagagcga ccacacaaca gtaaaatacc aggcgaacta cggtggtggc
     8641 tgcttaaatg ctgatggcaa acctgtcgtg atcaaagatt ggagcgcttt gggcactcct
     8701 gcaagctgta acggcccaac aaacttcgct gtcactggta t
//
LOCUS       DATBTE010000189        18292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1321657, whole genome shotgun sequence.
ACCESSION   DATBTE010000189 DATBTE010000000
VERSION     DATBTE010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 18292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18292
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321657"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VIG33_06900"
     CDS             <1..335
                     /locus_tag="VIG33_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8270601.1"
                     /translation="ECCRTKKSIIEGFCHIPNLSLEFGVADAVVLNDGHQFLTLIYPL
                     ENKHRIKMEYYREKNLTRSELNIVSLLLEGHSNSQIMERLFISKATLKTHINNIYKKI
                     PAEMRPRN"
     gene            342..2084
                     /locus_tag="VIG33_06905"
     CDS             342..2084
                     /locus_tag="VIG33_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEY8270602.1"
                     /translation="MENLKCSPTDVSLKSCFLGPKAENGDWVLEEVTAVFKRWFEWRR
                     ELFPEDGKAISSTDQQLKEFQSQRAHAQRNIENLLSRFENEIPKFAPRYIGHMFSEFS
                     LPAFFGHIITLIHNPNNISTESSKVGIQIEDEAIQNLADMLGMPTSFGHFTSGGTVAN
                     FECLTRAIERVNKWLFLGLEARRAQEKPALTVFEAAHMGWDEFSSLESQHGTLDLDWS
                     NPLVRARRIEKIFKLEEYAGPVLLVPYHKHYSWTKGASLFGLGEESLWPIELNEQGTL
                     SLESLRQSIEKAKKLHRPIAAVVSVLGTTEMGFVDPIDQVQQLLDQYQKEEGLYIWHH
                     VDAAFGGFFAVMKSMEALSKDLRRSLTGLSRVNSITIDPHKMGYVPYSSGAFLARQEE
                     DYYVKSFVAPYVQFQIQKDKGPFTLEGSRSAAGAVATWLTAQSIGFTEQGYGSILERT
                     IRGKNALEKSLQREPLIRLAPATGLNILGFCIAADGDSFEKVNQKTLALFQAISEAPS
                     PKFFVSKTKLYRKSYEDYLLRYAKTWSYQGEVGDLEVIRICIMNPFFDSKELNLDLKQ
                     EFSQYLSQEIKKLS"
     gene            2169..3431
                     /locus_tag="VIG33_06910"
     CDS             2169..3431
                     /locus_tag="VIG33_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY8270603.1"
                     /translation="MFNQELELLMPAGSLEKLKYAFNYGADAVYAGVPLFSLRARENE
                     FNLEDLKEGIHFARERNKKVYFTANIFARNLKLKPFTHQIKEFAELQPDALIMSDPGL
                     ISIVKEQHPELQIHLSVQANCMNWQSVKFWQKQGVSRVILSRELRLDEIKEIKDRVPD
                     IELEAFVHGSICIAYSGRCLLSHYMSHRDANQGVCDNSCRYGYNIHKAETEKEEHYYL
                     EDLRNKGEFYPIDEDENGTYIMNSKDLCLIEHLKEMAEAGVCSFKVEGRTKSVYYVAM
                     VSKIYRQALNDLKAGRPFNAELYKELDKVASRGYHKGFLMGNPTHEAQNYSHSTPRQY
                     SQKFAALLTTQPDSSVVAEVRNKMQLGDAVEVISPKTVVETSILGLQTLEGEPLKEAH
                     GGAGIYKIDLGVPVENHSLLSIKSGRPL"
     gene            3428..3730
                     /locus_tag="VIG33_06915"
     CDS             3428..3730
                     /locus_tag="VIG33_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEY8270604.1"
                     /translation="MRPPAKLADLRSRIDLVDRELVKLLLQRFELVGQILEEKRNSHL
                     PIRDAQREKEILEKLSRAVASAPCMEELVSIFQGILKVSCEYMQKKDRVDKAGSGD"
     gene            3757..4929
                     /locus_tag="VIG33_06920"
     CDS             3757..4929
                     /locus_tag="VIG33_06920"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEY8270605.1"
                     /translation="MQATEKPLKNSQENPMDAYRYFFPAGWLLGVWGVLLWLFFPLNL
                     VAYPGPFHPLVMMGGFFLCFVCGFLMTAAPKFTASFPPARSELWMSATLLAGLFISAL
                     CESSLVFHLAALLLLGFLVLFLARRFFHRTQSPPQAFVFVGVGMGCALLGVTLLILGD
                     LDWAADSWLHLGRLLFLQGYILALVLGVGSRLVPALLGRGPLPNANEGMKRSPAKLSL
                     FLILAFLFVGSYIVEVFLHEFAGTFLRSVVITFMFFQLWKIYLLPARKGYQPFWLWVS
                     AWFLLIGQWSATLWPDYRQHSLHLVFISGLGLMTLMIATRVSLSHGEHGLELEKNSKA
                     IVAGALLLILAALTRVSAGFSAVIYQSHLIYASCTWILGLLVWGWVYLPKMIFPKK"
     gene            5121..5369
                     /locus_tag="VIG33_06925"
     CDS             5121..5369
                     /locus_tag="VIG33_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8270606.1"
                     /translation="MGRRILPKSIPNTSNTLTTEILGQFVRAKRTQAQLRIDDAAQLC
                     GVSVETLSKIETAKSGVRVDSLFKVLNGLGIKLQVVPW"
     gene            5363..6661
                     /locus_tag="VIG33_06930"
     CDS             5363..6661
                     /locus_tag="VIG33_06930"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEY8270607.1"
                     /translation="MVTPAPPLKVYFEDLLVGELFYDINQNNFHFTYDSGWVEQKQFP
                     FSPHVPLTAPPAPGAVSRFLENLLPEGRGLKDLAQNLKVPLTNTYALIAAIGKETTGA
                     FSFIAGKERPDTRFRPIPKEELTQRIKERKARSLMIWDGRPRLSLAGVQEKLAVVIKN
                     KEYGLGEGNISSTHILKFGNEKVPHLVLNEYYCMQLANAVGIPVAQTQLVEFSERVLQ
                     VERFDRAWISEARVARHHIIDGCQALDISPDHKYERLFGDGPETADILGPASLINLYK
                     FCQQCLVPAKAQLQLLQWVIFNLLIGNSDHHVKNISYYIDQEGISMAPAYDLLNVTVY
                     PKFDQSLAFQIGDTFKLEEMNAYQFAEMANELGLAANFLAAQIKKISEKTLKSLDKIA
                     ISGLNAEENQFIGILKENIKTRTHQFLSESSKIKLYKLGH"
     gene            6681..6818
                     /locus_tag="VIG33_06935"
     CDS             6681..6818
                     /locus_tag="VIG33_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270608.1"
                     /translation="MSISKKDFEVLREKFNLAIKEVVDIAKESAAEEIVCLNIDFFRQ
                     S"
     gene            6950..7942
                     /locus_tag="VIG33_06940"
     CDS             6950..7942
                     /locus_tag="VIG33_06940"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY8270609.1"
                     /translation="MSREHKETHQSLENPYPDYVAELPADHPVRAYIEENTYLREILR
                     EIDETDAVTEFQKFFNLFNQLSEVEKHYARKENQLFPFLERHGWTGPSQGMWNFHDAI
                     RDLLRAVRKQIENKNVAGLNEEMRVIFAEMQRMMLIEETRLFPNALQLLTEEEWQQMR
                     AGDEEIGWMLKTKPISYSQETKVKPTSIGDRFRLDEGFLTPEQVNLMLKFMPVDLTYV
                     DENDRVLFYNRGEDRVFPRSAGIIGREVRFCHPPKSVGTVLRILEEFKKGTKNEAAFW
                     IQFKGRIIHIRYFAIRDREMKYKGVIEMSQDITEIQKLEGEQRLLDWENDNDHV"
     gene            7929..8732
                     /locus_tag="VIG33_06945"
     CDS             7929..8732
                     /locus_tag="VIG33_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270610.1"
                     /translation="MITFNAEEFNQRWLKARTSALRKVCEPSKDTIEFSEADLIDQYF
                     SQVAAATPEERIKKKDQLLRHVFDHSRLLAMSLSKFLGVTFEIQDFQDLLEKSEIPCV
                     QGQWDSRRTAKVLNRHGCDFCPKSGANACDYWREALDGLVMGLGDKERLARHASVRHG
                     DAACVDVFYFDAEEERRETSLAWGPIPESMTAKLLEICDDFESKMKTSILLKGLREGV
                     LYFEFKSSTDTLCSGGQLLASTFQRKIQKHYPALRIQEVTPRAVLGVEQ"
     gene            8729..9040
                     /locus_tag="VIG33_06950"
     CDS             8729..9040
                     /locus_tag="VIG33_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270611.1"
                     /translation="MNTMTRRDFFLGTFSKAETKTECIVGRISDFPIGKSKSLTALQV
                     EIESLPEGLRARSLENERCFYSIKMNQIGELIVNRRELWPENQAFSILTNEQTSLEDR
                     S"
     gene            9037..12516
                     /locus_tag="VIG33_06955"
     CDS             9037..12516
                     /locus_tag="VIG33_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY8270612.1"
                     /translation="MSEESKKGFSRRSFVKGMGITGGILGASSWVHATPLRYFKPTGI
                     ENPLAFYPHRDWESVYRNILAHDSSFVFLCAPNDTHNCLLKAYVKNGVVQRIGPSYGY
                     GKATDLYGNQASHRWDPRICQKGLALVRRFYGDRRVKAPMIRRGFKEWVDAGYPRDAQ
                     TGKPDAKYFQRGKDKWLRLGWEDAYKYAAKSMHNIAETYSGKKGEEYLKKQDYDPAMI
                     HALEGAGVQTLKFRGGMAFLGATRIFGLYRFANMLALMDSKIRGVGADKAIGGRGWDS
                     YSWHTDLPPGHPMVTGQQTNDFDLFAVERAKLLIVWGMNWITTKMPDSHWMTEARLKG
                     TKTVAVTVEYSATASKADDVLVIRPGTDPAFALGLCQHIMTKNLYDKDFVENKTDFPF
                     LVRLDTLQPLRPDEIIPGFKEPELNSLHFMKVGEKPPAGKEQKDQFVPESMREDFKSF
                     VIFDRKTKKYIAVSRDDYGDFAKKLKASPELKHQGNIKLVDGTSVKVRTIYSLTQEYL
                     NQNMTPKQVSQITGCPTSGIISLAEQIAKNKEKTIFACGMGPNQFFNADLKDRAIFMV
                     AALTRNIGFPGGNIGSYAGNYRTALIGGEPVYAAEDPFNLQMEKGGKPNVKKYTHYES
                     LHYFNYGDRPLRAGDKMFTGAGHMPVPTKAMWLNNSNSVIGNIKWHYDVVNNTLPKLE
                     FIAYSDWWWTGSCEYADLVFACDSWAEFKQPDMCGSCSNPFIQIFPSTPMERVFDTRA
                     DIEIIAGVSKALSKVTGDTRMMDMWKFVQDGNTHEYLQRIIDASATLKGYKIGDLEEK
                     AKQGIPALMNNRTYPRISSYEQARSEKPWHTKSGRLEFYRPEVEFIESGENMVIYREP
                     VDSTPHEPNVIVSKKHEALRAKQPADYGFDKKDVSSETRQVRNVVMTTEEMMKSQHPL
                     RLKNYAHIYHTPKYRHGAHSTPVDTDITSIWFGPFGDVYRHDKRMPSVVEGYVDINPL
                     DAKELGVEDGDYVYIDGDPEDRPFRGFKKGTEEYKVARLMLRARYYPGTPRGVTRTWH
                     NMYGATFGSIKGQETRADGLAKSPETNYQSMYRSGSHQSATRAWLRPTQMTETLVHKT
                     MFGQGIEKGFAADIHCPTGAPREAYVKITKAENGGMNGQGTWRPAALGYRPTYENKAM
                     KEYLKGSFMKRKG"
     gene            12520..13650
                     /locus_tag="VIG33_06960"
     CDS             12520..13650
                     /locus_tag="VIG33_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEY8270613.1"
                     /translation="MAKVKNWQIQREMEYPYQEHRPKRQWSIVFDLNKCIACQTCSVA
                     CKTTWTSGKGQEYMFWNNVETKPYGSYPLAWDVRVLEKLGQQAWKAKEAYYGKTIFEA
                     AGDNEVAAHFAPKDEDWMYPNNGEDDCLGNVSHGAHLTLPHDKWFFYLPRTCAHCTYP
                     ACLAACPRKAIYKREEDGIVLIDQSRCKGYGECVRACPYKKSMYNTHTRVSEKCVGCY
                     PAVEKGYQPMCVQNCIGKIRVMGFVNPPWKIREDNPIDYLIHKKEIALPYYPQLGLEP
                     NIYYIPPIHADRDYLLQMFGPKVDQAIANYKKMKTDPVAQGLMVLIGSSDRILHSFKV
                     KDGVAFGYDEKGDEIVSVPVTEPMFKRAAFDEKLKATRHNTP"
     gene            13666..14517
                     /locus_tag="VIG33_06965"
     CDS             13666..14517
                     /locus_tag="VIG33_06965"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HEY8270614.1"
                     /translation="MKKNLLLLTIIFSCSGANAQVYAVKKVAADLSKTPQVNGGYWQG
                     VAEIKIDLLAQMMVKPKPAKVQTETAKVQMVHDGKYIAFRIRWNDKEKSEAGKLGEFS
                     DAVALEFPVLDNQNPPPIFMGFKDNPVHLFHWRAQYQYDEMHGKKSVKDIYPNLNADT
                     YPNEFPDRGALKAVSEESKEIFSHGKAAGNPQSAQKKAVDELLAEGFGTSSVLKETDS
                     VAHSEWKEGEWTVVISRALKRPQGSVLEIGKGSSFGLAVWQGGQDEVASRKSITMQWT
                     AFKVEEK"
     gene            14518..15210
                     /locus_tag="VIG33_06970"
     CDS             14518..15210
                     /locus_tag="VIG33_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEY8270615.1"
                     /translation="MDVLEQTNPASFVLGSVLMSYPVDSISANIPILLNDIGLEISED
                     LRALILNFTSPENIQDLQSEYISIFDNGRDINPIYETEYDRRRAMAKGNELSDIAGFY
                     RAFGFELDSTAEGMEILDHVGIELEFYSLMLMKQLYLAETEDQQGMEIVEDGRKKFLT
                     AHLGRFVGAISRRPGVEKSEFYSQIFKWGAQLVEDECKRLHLTVVPADWIDSEVIKEE
                     DMNCSTGGSCLK"
     regulatory      15207..15351
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            15340..16323
                     /gene="moaA"
                     /locus_tag="VIG33_06975"
     CDS             15340..16323
                     /gene="moaA"
                     /locus_tag="VIG33_06975"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015467210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY8270616.1"
                     /translation="MRALLRDSYDRQFQYLRLSLTEVCNFRCSYCLPNGYKSSCSDFP
                     LGESEVFHLLQAFSELGISKVRLTGGEPTIRSDLVSLVQKISQIEGIDKIALTTNGYR
                     LQSLAAPLFEAGLTNLNVSIDTLQEDKFQKITGKSNLKNVLAGMEKAISVGIPAVKIN
                     TVLLKDLNDIELPEFMEFVRKVPVSWRFIELMQTGNNKDFFRQRHLSTKVIDSYLRDR
                     GWSEVSKQATDGPATEFSHPDYLGRIGIIAPYSKDFCKSCNRLRVSSRGALQLCLFGE
                     GQVNLRHLLQASEQKEELKSKIEKSLFLKSVGHELHSGLTGQTQNLSMIGG"
     gene            16334..17350
                     /gene="moaCB"
                     /locus_tag="VIG33_06980"
     CDS             16334..17350
                     /gene="moaCB"
                     /locus_tag="VIG33_06980"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF002947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HEY8270617.1"
                     /translation="MTFNSEFKMANVAEKISTSRRAIARGYIKMSPATAKIVAEKKLP
                     KGDALMMAEVAGILGVKKTIEILPLCHPLPIESVRVKCEPRGDGVVVSCEVITTYKTG
                     VEMEALMGVNAALLCIYDLTKPIDPVLSIHDIHLVMKEGGKSGLWQNPEFPQDPQIPL
                     SSNKSCALRKSLKGLKTAVLTISDRCSRKQAEDGSGPVLKQMLKEREAEILWSDLVPD
                     EISEIQKAITRFKESGADLIVSTGGTGFSPRDVTPEAVEPMLTRRVEGFGELFRAEGA
                     KRTPMSYLSRSVSGFIGETLIITLPGSPRACREGIDILAYLIPHAIHIAKGGPHGGGH
                     EISK"
     gene            17334..>18292
                     /locus_tag="VIG33_06985"
     CDS             17334..>18292
                     /locus_tag="VIG33_06985"
                     /inference="COORDINATES: protein motif:HMM:NF028293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate transporter family protein"
                     /protein_id="HEY8270618.1"
                     /translation="MKSVNNLSGDFLGSFADGAVLFPLLIMLSSLNGMNLSYLLLSSG
                     LTYLVAGFFFRVPMSVQPLKSIAFAGLAVGASGYEIRAAAVGLGLIFIASLRFDLESW
                     AKQVPIKLIHGVQVGLGFVLVQQGYKHVMSYGLEFILLAGVSVALIIYLTYKFQYPIL
                     GVVSSMGFFSSLFWLEKIDLPSGNVSLNLESFRPFLILSLVLPQIILTSANSVLATAN
                     VSRQLFGKEAERVNVRRLISLMGFGNVLSGLLGGLPFCHGSGGVTAHHKGGARTNRSN
                     YFIGTSLLFAALASFIFGNARVTFSPLLLSVLLLAVGFMHVVLA"
BASE COUNT         4993 a         3782 c         4525 g         4992 t
ORIGIN      
        1 atgaatgttg ccgaaccaaa aaatccatca tcgaggggtt ctgtcatatt cccaatctaa
       61 gtcttgagtt tggagtggcg gatgcggtcg tattgaatga tggacatcaa ttcctcacct
      121 tgatttaccc tctggaaaat aaacatcgca taaaaatgga atattaccgt gaaaaaaatc
      181 tgacgagaag tgagctgaat attgtttcac tgcttcttga aggacatagt aattcgcaga
      241 ttatggagcg cctttttatt tccaaggcca ccttgaaaac acatatcaat aatatttata
      301 aaaaaattcc agctgaaatg agaccacgaa attaaggact tatggaaaat ttaaaatgct
      361 caccaacgga tgtttctctt aaaagttgtt ttctgggtcc taaagccgaa aatggggact
      421 gggtccttga agaggtcacc gctgttttta aaagatggtt tgagtggcgg agggagcttt
      481 ttcctgagga tggaaaagcc atctcctcaa cggatcagca gttaaaagaa tttcaaagtc
      541 aaagggccca tgcgcaaaga aatatcgaga atctattaag ccgcttcgaa aatgaaattc
      601 ccaagttcgc gccacgctat atcggtcata tgttctctga attttcctta cccgcttttt
      661 ttggccatat catcactctc attcataatc ccaataatat ttctactgaa tcctcaaagg
      721 tgggaattca aatcgaggac gaagccattc agaatctggc ggatatgctc ggaatgccca
      781 caagctttgg ccattttact tccggtggaa cggttgccaa ttttgaatgc ctgaccaggg
      841 cgatcgaaag agtgaacaag tggctttttc tgggcttgga ggcgcggcgc gcacaggaaa
      901 aaccagcgct gaccgtattt gaagcggctc atatgggatg ggatgagttt tcttcactgg
      961 aatctcagca cggaaccctg gacctggatt ggagcaatcc cttggttcgg gctcgccgta
     1021 ttgaaaaaat atttaaactc gaagagtatg cggggcccgt tttacttgtc ccttatcata
     1081 agcattactc atggacgaaa ggggcgtctt tgtttggcct tggggaagaa agcctttggc
     1141 cgattgaatt gaatgaacag gggactctca gtcttgaaag tctccgtcag tcgattgaga
     1201 aagccaaaaa actccatcga cccattgccg ccgtggtttc ggttttggga acgacggaaa
     1261 tgggtttcgt cgatcctatt gatcaggtcc agcagctcct ggatcaatat caaaaagaag
     1321 aaggtcttta tatttggcat catgtggatg cggctttcgg tggctttttt gcggtgatga
     1381 agtctatgga agccctgagt aaggatttaa gacgctcttt aactggcttg tcgagagtga
     1441 attctatcac cattgatccg cacaaaatgg gctatgttcc ttattccagc ggggcttttt
     1501 tagctcgtca ggaggaggat tattatgtga agtcctttgt ggcaccttat gttcagtttc
     1561 aaattcaaaa agacaaaggc cctttcaccc tggagggctc acgctcggcc gcaggagccg
     1621 tggcaacttg gctgacggcg caatccattg ggtttaccga acaaggttac ggaagcatcc
     1681 ttgagcgcac catccgtgga aaaaatgccc tggaaaaatc tttacagcgc gaacctctga
     1741 ttcgcttggc tcccgccact ggcttgaata ttttaggctt ctgtatagcc gctgacggcg
     1801 attcttttga gaaagtaaat caaaaaaccc tggccttatt tcaggcaata tctgaggcac
     1861 cgtctccgaa gtttttcgtt tcaaaaacaa agctctatag aaaaagctat gaggattatc
     1921 ttctaagata tgcgaaaacc tggtcctatc aaggggaagt gggggatttg gaagtcattc
     1981 gaatttgcat catgaatcct ttctttgact ctaaagagct taacctcgac ctcaagcagg
     2041 agttttcaca gtatctttcc caagaaatta agaagctgtc ataaaaatcc acccgggggt
     2101 ggtgatgttc aaacgagaga tccttcttta tacatagctt ctaaatttct tcaaaaagcg
     2161 gagatgctat gttcaatcaa gagctagagt tgctgatgcc tgccggttcc ctggaaaaat
     2221 taaaatacgc ttttaattat ggggctgatg cggtttacgc gggcgttcct ttgttctcac
     2281 tccgtgcccg ggaaaatgaa ttcaatctag aggatttaaa agagggtatt cactttgcgc
     2341 gcgagcggaa taagaaggtc tattttacgg cgaatatttt tgcgcgcaat ttaaagctca
     2401 agcccttcac ccatcaaatc aaggagtttg cggaattgca gccggatgct ttgattatga
     2461 gtgatcctgg cctcatcagc attgtgaaag agcagcatcc tgagttgcaa attcatcttt
     2521 cggtgcaagc gaactgtatg aattggcaat ccgttaagtt ttggcaaaag cagggcgtga
     2581 gtcgagtcat cctgagtcgc gagcttcgct tggatgaaat caaggagatc aaggaccgcg
     2641 ttccagatat cgaacttgag gccttcgttc atggatcgat ttgtattgcc tattcgggcc
     2701 gctgcctgct ctcccattac atgagtcatc gggatgcgaa ccagggggtc tgtgataatt
     2761 cttgtcgtta tggatataat atccataaag cagagactga aaaagaagag cattactatc
     2821 ttgaagacct cagaaacaag ggcgaatttt accccatcga tgaagatgaa aatgggacct
     2881 acatcatgaa ctccaaagat ctttgcctga ttgagcatct caaagagatg gctgaggcgg
     2941 gtgtatgctc cttcaaggtt gaggggcgta cgaaatcggt ttattatgtg gcgatggttt
     3001 caaaaattta ccgccaagct ctgaatgacc tgaaagccgg acgtcctttc aatgcggaac
     3061 tctataaaga actcgataag gtcgcgagcc gcgggtatca caaaggtttc ctgatgggaa
     3121 atccgaccca tgaggctcag aattactcgc attcgactcc acgtcagtat tcacaaaaat
     3181 ttgcggcttt gttgaccacg caacccgact ccagtgtggt ggcggaggtg cgcaataaaa
     3241 tgcagctagg ggatgctgta gaggtgatat caccaaagac tgtggtggag acttctattc
     3301 tcggactgca aaccctggaa ggagagcccc ttaaagaggc gcatggggga gccggaattt
     3361 ataagataga cttaggagtg ccagtggaaa accattctct tctgagcatc aagtctgggc
     3421 ggccgctttg aggccaccgg caaagcttgc ggatctgcgt tcgcgaatcg atctcgtgga
     3481 ccgtgaactg gtcaagttgc ttttgcagcg atttgaattg gtaggccaaa tccttgaaga
     3541 aaaaagaaat tctcatttac ctattaggga tgctcagcga gaaaaagaga ttcttgaaaa
     3601 gctcagtagg gctgtggcct cggcgccctg tatggaggag ttggtttcta tttttcaagg
     3661 aatactgaaa gtgagttgcg agtatatgca aaaaaaagat agagtcgata aagcaggtag
     3721 cggagactaa aatcggagac caaaaaagga cagtgaatgc aagcaacaga gaagcctcta
     3781 aagaactcac aagagaaccc gatggatgct tatagatact ttttcccggc gggatggttg
     3841 cttggagtat ggggcgttct tctttggctt tttttcccct tgaatttagt ggcttacccc
     3901 gggccttttc atcctctggt tatgatggga ggtttttttc tgtgctttgt ttgcggattt
     3961 ttgatgaccg cagcgcctaa attcaccgcg agctttcctc ctgcgagaag tgaactttgg
     4021 atgtccgcga cacttttggc cggtcttttt atcagcgcct tgtgtgagag tagtttggtt
     4081 tttcatttgg cggctttgtt gttgttgggt tttttggttt tatttttagc gcgacgtttt
     4141 tttcatcgaa ctcagtcgcc tccgcaggca tttgtgtttg ttggcgtcgg catgggctgt
     4201 gctcttctgg gagtcactct cttaattttg ggagaccttg actgggcggc cgattcttgg
     4261 ttgcatttag gtcgtctcct ttttctccaa ggatatattt tagctctggt cttgggtgtc
     4321 ggaagccgct tggtgccagc gcttttggga cgcggaccac tgcccaatgc caatgaaggc
     4381 atgaagagat cgcctgcgaa attgagtctg ttcttgatac tcgctttttt atttgtaggg
     4441 tcttatatcg tagaagtgtt tttgcacgaa tttgcaggga cttttttaag atctgtcgta
     4501 ataactttta tgttttttca gctctggaaa atttatctcc ttcctgcccg caaagggtat
     4561 caaccctttt ggttgtgggt ttccgcttgg tttttgctga tagggcagtg gagcgctact
     4621 ttgtggccgg actatcgcca gcacagcctt cacttagttt ttatttcagg ccttgggttg
     4681 atgaccttga tgatcgccac acgggtgagt ctttctcatg gggagcatgg cttggaactc
     4741 gaaaaaaatt ccaaggcgat cgtggcgggg gctttactct tgatattggc ggcgctcaca
     4801 agagtttcag cgggcttctc cgccgtgatc tatcaatccc acttgatata tgcttcttgc
     4861 acctggattt tgggtttgtt ggtctggggc tgggtctact tgccgaaaat gatttttcct
     4921 aagaagtgaa actcgtttga gcttagatcg taagggctgg tcgtggttta tccgtcctct
     4981 aaaggcttaa tttctatttt tatagaaatt aagcctttaa gcccctcatt taactcatta
     5041 tttctataaa aatggaaact tattgaaaaa tgaccacatg aagcctataa tttctatata
     5101 tgtagaaaca aaggaaagct atgggccgcc gtattttacc taaatcaatt ccaaatacct
     5161 ctaatactct cactaccgaa attctagggc aatttgttcg cgccaaaagg acccaagcac
     5221 aactcagaat tgatgatgct gcccaacttt gtggggtctc tgtagaaact ctctccaaaa
     5281 ttgaaacggc aaaatcagga gtgagagtag acagtctatt taaagttctt aacggattag
     5341 gcatcaaact tcaggtggtt ccatggtaac cccagcccct ccactgaagg tgtattttga
     5401 agatttattg gtcggagaac ttttctacga catcaatcaa aacaattttc attttaccta
     5461 tgacagcggt tgggtggaac aaaaacaatt tcctttttcc cctcacgtcc ccctcacagc
     5521 tcctcccgct cccggagcgg tctcccgctt tttagaaaat ttgcttcccg aggggcgagg
     5581 attaaaagat ctcgcacaga acctcaaagt tccactcacc aatacttatg cccttattgc
     5641 agccattgga aaagaaacaa caggcgcttt ctcttttata gctggaaaag aacggccgga
     5701 cacaagattt cgtcctattc cgaaagaaga acttacccaa cgtataaaag aacgaaaagc
     5761 tcgatccttg atgatttggg atgggcgtcc tcgactttcc cttgcaggag ttcaagagaa
     5821 gttagcagtt gttattaaga ataaagaata tggacttgga gaaggaaata tttcatccac
     5881 tcatatcttg aagtttggaa atgaaaaagt ccctcatctg gtattgaatg aatattactg
     5941 catgcagctc gcaaatgcag tcggaattcc cgtggcacag acccaacttg tcgagttctc
     6001 tgaacgtgtg ctgcaagtgg agcgcttcga ccgcgcttgg atcagtgaag cccgcgttgc
     6061 tcgccaccat atcattgatg gttgccaggc cttggacatt tcgcctgatc ataagtatga
     6121 gcgtctcttc ggggacggac ctgaaaccgc ggatatttta ggacccgcca gtcttataaa
     6181 tttatataag ttctgtcagc aatgtttggt cccggccaaa gcgcagttgc aacttcttca
     6241 gtgggttatt tttaaccttt taatcgggaa cagtgaccac cacgtaaaaa acatctctta
     6301 ttatatcgat caagagggaa taagtatggc tccagcctat gatcttttga atgtgactgt
     6361 ttaccctaag tttgatcaat cgttggcttt tcaaatcgga gataccttca aattggaaga
     6421 gatgaatgca tatcaatttg cagaaatggc gaatgagttg ggtctcgcag cgaattttct
     6481 ggcagcacaa ataaaaaaga tttctgaaaa aacattgaag agccttgata aaattgcgat
     6541 ctcaggtttg aacgcagaag aaaaccaatt cataggcatc ttgaaagaaa acattaaaac
     6601 tcgaactcat caattcttat ctgaatcctc aaagataaag ttgtataaat tgggtcatta
     6661 atcttttctt tacttcaccc atgtcgattt caaaaaaaga ttttgaagtt ctgcgcgaaa
     6721 aattcaatct cgccatcaaa gaggttgtgg acattgctaa agaatcagca gcagaagaga
     6781 tcgtgtgctt aaatatcgat ttcttccgac agagttaagg catgctctta agctcagtgg
     6841 ttttatctga aatgaagatc tgacatgatc caggacatag ttctttagaa ttcaatttga
     6901 aattttctcc gaattgccat tatttgatgg ttgtgaggag aactcgaaaa tgagtcgcga
     6961 gcataaagaa acccaccagt ctttggaaaa tccttaccca gactatgttg ctgagcttcc
     7021 ggcggatcac ccagtcagag cttatatcga agaaaatacc tatcttcgag aaattttaag
     7081 agaaatagac gaaaccgatg ccgtcactga atttcaaaag ttcttcaatc tctttaatca
     7141 attgagtgaa gttgaaaaac actatgcaag aaaagaaaat cagctttttc catttcttga
     7201 aaggcatgga tggaccggtc caagccaagg aatgtggaat tttcatgatg ccatcaggga
     7261 tttgttaaga gctgtccgaa agcaaataga aaataaaaat gtcgcaggtc tcaatgaaga
     7321 aatgagagtc attttcgcgg aaatgcagag aatgatgctg atcgaagaga cgagattgtt
     7381 tccgaatgca ttgcagcttt tgacggaaga agagtggcaa caaatgcgtg caggagacga
     7441 agagattggt tggatgctta aaaccaaacc catttcctat tcccaggaga caaaggtaaa
     7501 gcccacctcc ataggagatc gtttccggct tgatgaaggc tttctgaccc ctgaacaagt
     7561 gaatttaatg cttaagttta tgccggtgga tttaacctat gtggacgaaa acgaccgggt
     7621 cctattttat aatcgcggtg aagatcgtgt ttttccccgc agtgccggga tcattggcag
     7681 ggaagttcga ttttgtcacc ctcctaaaag tgtggggacc gttttgcgga ttttagagga
     7741 gtttaaaaag ggcactaaaa atgaggccgc gttttggatt caatttaaag gtcgcatcat
     7801 tcacataaga tattttgcga tccgggaccg ggagatgaaa tataagggcg tcattgaaat
     7861 gtcccaagat attactgaaa ttcaaaagtt agagggagag cagagacttt tggattggga
     7921 gaacgacaat gatcacgttt aatgcggagg agttcaatca gcgctggtta aaagcgagaa
     7981 cctctgctct ccgtaaggtt tgtgaaccaa gtaaagatac cattgagttt tctgaggctg
     8041 atcttatcga tcagtatttt tcacaagtgg cggcagcgac acccgaagag agaattaaaa
     8101 aaaaggacca gctgcttcgt cacgtgtttg accacagccg acttctggcg atgagccttt
     8161 ccaagttctt gggagtgact tttgaaattc aagattttca agatctttta gaaaagtctg
     8221 aaattccctg cgttcaaggt caatgggact ctcgcaggac ggcaaaagtt ttaaaccgcc
     8281 acggatgtga tttttgtcca aagtccggcg cgaatgcctg tgattattgg cgggaggctt
     8341 tagatggact ggtgatgggg ttgggtgaca aggaaagact cgctcgccat gccagcgttc
     8401 gtcatgggga tgccgcctgc gtggatgtct tttatttcga tgccgaagag gagcgcaggg
     8461 aaacctccct ggcctgggga ccaattcctg agtctatgac cgcaaagctt ctcgagattt
     8521 gtgatgattt cgaaagtaaa atgaaaacct ccattttact caaaggcttg agggaaggtg
     8581 ttttgtattt tgagtttaaa tcctccaccg acacactttg cagcggtggt cagcttttgg
     8641 cttctacttt tcagcgaaag attcagaagc actatcctgc tttgcggata caggaagtga
     8701 ctcctcgtgc ggtcttagga gttgaacaat gaataccatg actcgtcggg atttttttct
     8761 aggaactttt tccaaagctg agacaaagac agaatgtatc gtcggtagaa tatctgactt
     8821 tcctattggc aaaagtaaat cactgaccgc acttcaggtg gaaatcgaat ctttaccaga
     8881 aggcttgaga gcgcgttccc tggaaaatga acgctgtttt tattcaataa aaatgaacca
     8941 aattggagag ctcatcgtca atcgcaggga actatggccc gaaaaccaag ctttctcgat
     9001 cctgacgaac gagcagacat cattggagga ccgatcatga gcgaagagag caaaaaagga
     9061 tttagtcgcc gaagctttgt taaaggaatg ggtatcaccg gtgggatact tggagcatcc
     9121 agttgggttc atgccactcc gctgaggtat tttaagccga cagggataga aaatcccttg
     9181 gctttttatc cccatagaga ttgggagagt gtttatcgaa atattcttgc ccatgattca
     9241 agttttgtat ttttatgcgc tccgaacgac acccataact gtcttttgaa agcctatgtt
     9301 aaaaacggcg tcgtgcaaag aatcgggcca agttatggct atggcaaggc gacagacctt
     9361 tatggcaacc aagcctcaca ccgttgggat ccgcgcatat gccaaaaggg cttagcctta
     9421 gtacgccgat tttatgggga tcgtcgggtg aaagcgccga tgatacgtcg tggatttaaa
     9481 gagtgggtgg atgcgggtta tccacgggat gctcagactg gaaaaccaga cgcaaaatac
     9541 ttccaaagag gcaaagacaa gtggcttaga ctgggatggg aagatgctta taagtatgcg
     9601 gccaagtcca tgcataatat cgcagaaact tattctggaa aaaaaggtga agagtatctt
     9661 aaaaagcaag actacgatcc cgcgatgatc catgccctgg aaggggcggg tgtacaaacg
     9721 ctcaagttcc ggggcggcat ggctttcttg ggtgccacgc gcatcttcgg tctttatcgg
     9781 tttgccaata tgctggcgtt gatggattca aaaattcgtg gtgtcggagc tgataaagcc
     9841 atcggtggcc ggggttggga ttcttattct tggcatacgg acttgccccc gggtcatccg
     9901 atggtgacgg ggcaacagac caatgacttt gatctttttg ccgtcgagcg agcaaaactg
     9961 ttgattgtct ggggcatgaa ttggatcacc acaaaaatgc cggattctca ctggatgacc
    10021 gaagcgcggt taaaaggaac caaaaccgta gctgtgactg tcgagtacag cgcgacggca
    10081 tcaaaggccg atgacgtttt ggtgatacgc ccggggacgg atccggcctt tgcgttaggt
    10141 ctttgtcagc atattatgac caagaatctt tatgataaag attttgtaga aaacaaaaca
    10201 gactttccgt ttttagttcg cttggatacc ttacagccgt taagacctga tgaaattatt
    10261 cctggtttta aagagccgga gttgaactct ctccacttta tgaaggttgg agaaaagccc
    10321 ccggcaggca aggagcaaaa agatcaattt gttccagagt ccatgcgcga ggattttaag
    10381 tcttttgtga tttttgatcg taaaactaaa aagtatatag cagtctcgcg ggatgattat
    10441 ggcgatttcg caaaaaaatt aaaggcatcg ccagaattaa agcatcaggg caatataaaa
    10501 ctggtggatg gcacgtcggt gaaagtccgc accatttaca gtttgactca agaatatctc
    10561 aaccaaaaca tgactccaaa gcaagtttcg caaatcaccg gatgtcctac atcaggtatc
    10621 atcagcttgg cggagcaaat cgctaagaat aaagaaaaaa cgatttttgc ctgtggcatg
    10681 gggccgaatc agttcttcaa tgcggatctt aaagatcggg ccatttttat ggtggcggcc
    10741 ttaaccagaa atattggctt tccggggggc aacattggca gctatgccgg gaattatcgc
    10801 accgccctta tcggcggcga gcccgtttac gcagcagaag atccttttaa tctgcaaatg
    10861 gaaaaaggcg gtaaacccaa tgttaagaag tacactcact atgagtcttt gcattatttc
    10921 aactatgggg atcgaccctt gcgcgcaggg gataagatgt ttacaggcgc cgggcatatg
    10981 ccggttccta ccaaagccat gtggctgaac aactcaaact ccgtgatcgg caatatcaaa
    11041 tggcattatg atgtcgtgaa caatactttg ccgaaacttg aattcattgc ctactctgat
    11101 tggtggtgga ccggatcttg tgaatacgcg gatcttgtct ttgcctgtga ctcctgggca
    11161 gagtttaaac agcctgatat gtgtggttcc tgttctaatc cattcattca aatattccca
    11221 agcacaccta tggaaagagt ttttgatacc cgtgcggaca tcgagattat tgcgggtgta
    11281 tccaaagcac tcagtaaagt gaccggcgat actcgtatga tggacatgtg gaagtttgtc
    11341 caagatggca acacccatga atatttacag agaattattg atgcctccgc gactctcaag
    11401 ggatacaaaa taggagatct tgaagaaaaa gcaaaacaag gcatccctgc cctgatgaac
    11461 aatcggacct atccgcggat ttcttcctac gagcaagcaa ggtccgagaa accttggcac
    11521 accaagtccg gtcggttgga gttctatcgt cctgaggttg aattcatcga gagcggagag
    11581 aatatggtga tttaccgaga gcctgttgac tccactcccc atgaacccaa tgtgatcgta
    11641 agtaaaaaac atgaagcgct ccgggcaaaa cagcctgcgg attatggctt tgataaaaaa
    11701 gatgtcagct ctgaaacccg tcaagttcgc aatgtggtca tgaccactga ggaaatgatg
    11761 aagagccagc atcctttgcg tttaaaaaat tatgcccaca tttaccatac tcctaaatat
    11821 cgccatgggg cgcactccac acctgtggat acggatataa catcgatttg gtttggcccc
    11881 tttggcgatg tctatcgcca tgataagcgc atgccttccg tggtggaggg ctacgtcgat
    11941 atcaatcccc tcgatgccaa ggagttggga gtggaagatg gggattatgt atacattgat
    12001 ggcgatccag aggatcgccc cttccgcgga tttaaaaaag ggactgaaga gtataaggtg
    12061 gctcgcttga tgttgcgagc gcgatattat cccggcactc ctcgaggagt gactcgaacc
    12121 tggcacaata tgtacggtgc gacttttggc agcattaaag ggcaagagac tcgagcggat
    12181 ggcttggcga aaagtccaga aaccaattac cagtccatgt atcgcagtgg atctcatcaa
    12241 agcgcgactc gcgcctggtt gcgacccact cagatgaccg agactctggt tcataaaacc
    12301 atgtttggac aaggaattga aaaaggtttt gctgcggata ttcactgtcc tacaggagct
    12361 ccgcgggaag cctacgtgaa aatcacaaaa gccgagaacg gcggcatgaa tggccaagga
    12421 acatggcggc cggcggcttt gggttatcgt ccaacttatg aaaataaagc aatgaaggaa
    12481 taccttaagg gctcctttat gaagaggaag ggataagtta tggcaaaagt aaaaaattgg
    12541 caaatccaac gcgaaatgga atatccctac caagaacatc gccctaaacg tcaatggtct
    12601 atcgtttttg atttgaacaa atgcattgcc tgtcagacct gctcggtggc ctgtaagacc
    12661 acttggacca gcggtaaggg ccaagagtac atgttttgga acaacgtcga aacaaagccc
    12721 tatggatctt atcctttggc ctgggacgtg cgagtgcttg aaaaacttgg tcaacaagct
    12781 tggaaggcta aggaagctta ttacggaaaa actatttttg aagcggccgg tgacaatgaa
    12841 gtggcggcgc acttcgcgcc gaaagacgaa gactggatgt atcccaataa tggcgaggac
    12901 gattgtttgg gcaacgtttc tcatggggct catctgacgc tcccgcatga taaatggttt
    12961 ttttatctgc cacgaacttg tgcccactgc acttatcccg cgtgtttggc ggcgtgccct
    13021 cgcaaggcca tttacaagcg cgaagaagat ggaattgtgt tgattgacca gtctcgctgc
    13081 aaaggttacg gggagtgtgt tcgcgcctgc ccgtataaaa aatcaatgta caatacgcac
    13141 acgcgggtca gtgaaaaatg cgtggggtgt tatcccgccg ttgaaaaggg ttaccagccg
    13201 atgtgtgtcc agaactgtat cgggaaaatc cgcgtcatgg gttttgtgaa tccaccctgg
    13261 aaaattcgcg aagacaatcc gatagattat cttatccata aaaaggaaat cgccttgccc
    13321 tattatccac agttgggatt ggagccgaat atttattata tcccgcccat tcatgccgac
    13381 cgggattatt tactgcagat gtttggtccg aaggtggatc aggccattgc caattataaa
    13441 aaaatgaaaa ccgaccccgt ggctcagggg ctcatggtgc tgatcggcag ctccgatcga
    13501 atcctccaca gcttcaaagt caaagacgga gtggcctttg gttatgatga aaagggggat
    13561 gaaattgtga gcgtgcccgt gacggaaccg atgtttaagc gagcggcttt tgatgaaaaa
    13621 ctcaaagcga cccggcataa cacaccttaa aatgtgagga ctaggatgaa aaaaaatctt
    13681 ttgttactga ctataatttt ttcttgcagc ggagccaacg cgcaagttta tgcggttaaa
    13741 aaagtggctg cggatttatc aaaaactccg caggtcaatg ggggttattg gcaaggcgtc
    13801 gcagagatta aaatcgactt gttagcccag atgatggtta agcctaagcc tgcaaaagtg
    13861 caaactgaaa cagccaaagt gcagatggtt catgacggca aatatatcgc ttttagaatt
    13921 cgctggaatg acaaagaaaa atcggaagcg gggaagctcg gggagttttc agatgcggtc
    13981 gctcttgaat tcccggtttt agacaatcaa aatccacctc ccatatttat gggctttaaa
    14041 gacaatcctg ttcatctttt tcactggcgg gctcagtacc agtatgatga aatgcatggg
    14101 aaaaaaagcg tgaaagatat ttatccgaat ttaaatgcgg acacctatcc aaatgaattt
    14161 ccggatcgcg gagcgctcaa agctgtcagt gaggaaagca aagagatttt ttcccatggc
    14221 aaggccgcag gaaatccgca gtcggctcag aaaaaagccg tggatgaact gctggcagaa
    14281 ggctttggaa cctcgagcgt actcaaagag acagactctg tggcgcatag tgaatggaaa
    14341 gagggggagt ggactgtggt gatctctcgt gcgctgaagc gccctcaggg atctgttttg
    14401 gaaattggca agggatcttc atttggactt gctgtatggc aagggggaca agatgaagtg
    14461 gcgtctcgga aatccataac tatgcagtgg accgctttta aagtggagga gaaataaatg
    14521 gacgttctag aacaaacaaa tccggcgagt ttcgttttag gctcggtctt gatgtcctac
    14581 ccggtggatt ccatttccgc taatattccc attcttttaa atgatattgg tttggagata
    14641 agtgaagatc ttcgtgcttt gattctcaac ttcacttcgc ccgaaaacat tcaagacctc
    14701 caatctgaat atatttcaat cttcgacaat ggacgcgata tcaatccgat ttatgaaact
    14761 gagtatgacc gtcgtcgagc catggccaaa ggcaatgagt taagtgatat tgcaggattt
    14821 tatagagctt ttggatttga gttggactcc acagcggaag gaatggagat acttgatcac
    14881 gtggggatcg agcttgaatt ttattctttg atgttgatga aacagctcta ccttgcggag
    14941 actgaggacc aacaaggcat ggaaattgtc gaagacggac ggaaaaagtt tttaacggcg
    15001 catctaggcc gtttcgtagg ggcgatcagt cgcaggcctg gtgttgaaaa gagtgaattc
    15061 tatagtcaga tatttaagtg gggcgcgcag ttagtggaag acgaatgcaa gcgattgcat
    15121 ctgaccgtgg tgcctgcgga ttggatcgat agtgaagtga ttaaagaaga ggatatgaat
    15181 tgcagcaccg gtgggagctg cctgaaataa ttttttaact ccgagctgct tgagctaaag
    15241 cttctatttt ttaaaagcca ggctcctgca gccgtgaaca aaaatgttca ccagggtttt
    15301 gtgtggaaac gccaaagcct cccaatggaa aggagatcta tgagagcact gcttcgcgac
    15361 tcttatgatc gtcaatttca atatcttaga ctgtcgttga cagaggtttg taattttcgc
    15421 tgcagctatt gcctgcctaa cggatataaa tcttcttgta gcgattttcc tctgggtgaa
    15481 agcgaggtct ttcatcttct tcaagctttt tctgagcttg gaatcagcaa ggtgcgcctg
    15541 accggtgggg aacccaccat tagatcggac cttgtctctt tagtgcaaaa aatttctcag
    15601 attgaaggaa tcgataaaat cgccctcacc acaaatggct accgccttca atcattggcg
    15661 gcgcctttgt ttgaagccgg tttgaccaac ctgaatgtca gtattgacac ccttcaggaa
    15721 gataaatttc agaagatcac cggtaaatca aatttaaaaa atgtattagc gggcatggaa
    15781 aaagccattt cagtggggat tccggccgtt aaaataaata ctgttttact gaaagattta
    15841 aatgatattg aattgcctga gtttatggaa ttcgtgcgga aagttccggt gagttggcgt
    15901 tttattgagc tgatgcaaac aggcaataac aaggattttt tcaggcaacg tcatttatca
    15961 acgaaagtca tcgattcata tttaagagat cgcggctgga gcgaagtttc aaaacaggcg
    16021 accgatggcc cggccacgga attttcccac ccggattatt taggacgtat tggaattatc
    16081 gcaccgtatt cgaaggattt ttgcaaatca tgcaatcgcc tgagggtcag cagtcggggc
    16141 gctctgcagc tttgcctgtt tggagaaggg caagtcaact tacgtcatct gttacaagct
    16201 tctgagcaaa aggaagagtt aaaaagtaaa atcgaaaaat ctttattttt aaagtcagtc
    16261 ggacatgaac tccactctgg actcacggga cagactcaga atttatcaat gattggcgga
    16321 taggaaagta cttatgacat tcaattcaga attcaaaatg gcaaatgttg cagagaaaat
    16381 atccacttct cgtcgtgcca ttgccagggg ttatataaaa atgtcgcctg cgacagcgaa
    16441 gatcgtagca gaaaaaaaac tccccaaggg agatgccctc atgatggcgg aggtggctgg
    16501 aattttagga gtgaaaaaga ccattgagat tctgccgctt tgtcatcccc tgcccattga
    16561 atctgttcgg gtaaagtgtg aaccccgtgg cgacggggtt gtcgtctcct gcgaagtcat
    16621 cacgacctat aaaacagggg tggagatgga agccctgatg ggggtgaatg cggctcttct
    16681 ctgtatttat gatctgacca aaccaataga tcctgttttg agcatccacg atattcattt
    16741 ggtaatgaaa gaagggggca agtcggggct atggcaaaac cctgaatttc ctcaagaccc
    16801 acaaattcct ctatcttcga ataagagttg cgccctcagg aaatctttga aaggtctaaa
    16861 gacggccgtt ttaaccatca gtgatcgctg ttcaagaaag caagcggagg atggttccgg
    16921 ccctgtttta aaacaaatgc ttaaggaacg tgaggcggaa attttatgga gcgatctggt
    16981 accggatgaa atttctgaaa ttcaaaaagc catcactcgc tttaaagaat cgggggctga
    17041 tttgattgtc tcgacggggg gcacgggctt tagtccacgt gatgtgaccc ccgaagctgt
    17101 cgagccgatg ttgacgagaa gagtggaagg ctttggtgaa cttttccggg ccgagggcgc
    17161 gaaaagaacg cctatgagct atttgagccg aagcgtgtct gggtttattg gggaaacgct
    17221 aatcatcact ttgcctggga gtccaagggc ctgtcgggag gggattgata ttttagctta
    17281 tcttattccg catgccattc acattgccaa gggcggtccc catggaggag gacatgaaat
    17341 cagtaaataa tctttcaggt gattttttag gatcttttgc ggatggagct gtgctgttcc
    17401 ccttgctgat aatgctctca tctctcaatg gaatgaatct ctcctatttg cttttaagca
    17461 gtggcttaac ctatttggtc gctggatttt tttttcgtgt tccgatgtcc gtgcagcccc
    17521 ttaaatccat tgcctttgcc ggtcttgccg tcggggccag tggctatgaa attcgtgcgg
    17581 cggcagtggg tctcggtttg atttttattg cgagcctgcg ctttgatctg gagtcttggg
    17641 cgaaacaagt tccaataaaa cttatccatg gtgtgcaggt aggacttgga tttgttctcg
    17701 tacagcaggg ctacaagcat gtgatgtcct atggcttgga atttattctt ctcgctggag
    17761 tgagcgtcgc cttaataatt tatttaacct acaagtttca gtatcctatt ttgggagttg
    17821 tttcttcaat gggttttttt tcgtctttat tctggttgga aaaaatcgat ttaccctcag
    17881 gaaatgtttc attaaatctg gaaagtttcc gaccattttt gattttatcc ttggtcttgc
    17941 ctcaaataat cctgacatcg gcgaattctg ttttggcgac tgcgaatgta tcccgtcaat
    18001 tatttggaaa agaggctgaa agagtgaacg tccgtcgttt gattagtctg atgggctttg
    18061 gcaatgtttt aagcgggctg ctgggtggct tgccgttttg tcatggttca ggaggcgtga
    18121 cagcccatca taaaggggga gccagaacaa atagatcgaa ctattttatc ggtacgagtc
    18181 ttctctttgc ggcactggcg tcttttattt ttggcaatgc gcgggtgacg ttttccccat
    18241 tgttactaag tgtccttttg ctggctgtgg gtttcatgca tgttgtcttg gc
//
LOCUS       DATBTE010000190        20850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_93040, whole genome shotgun sequence.
ACCESSION   DATBTE010000190 DATBTE010000000
VERSION     DATBTE010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 20850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20850
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93040"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="VIG33_06990"
     CDS             <1..1337
                     /locus_tag="VIG33_06990"
                     /inference="COORDINATES: protein motif:HMM:NF033424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protease-like activity factor CPAF"
                     /protein_id="HEY8270619.1"
                     /translation="ATSFPYKVGDEVLAMDEIPVNQLRSSLTKDMKSTAPLTDQALGD
                     MFLTSRRGQRNQLVPRNTVTLTLLRAGESIPRTHQLNWEYTAEQIPGAETLPSQNQFN
                     KQLSFHGHFKLAQAQMLSPVADMFAQQDNFIDSPYGLGNKKPFLPDFGPRVWETDATN
                     TFDAYIYQNETSQLIGVIRIASYSPNSEDPKGREKAVADFSEILKKMQKLTSGLVIDQ
                     TNNPGGSIFYLYSLVSMLTDQAFYTPKHRMSIDTMEVKPCVEFEKAFEKISNDEEARK
                     LLGESDDGFPVNYEYAVAAKNYCQFMVTEFKQGKKITEPFYIFGVDKVNPSPVAQYLK
                     PIVILVNELDFSGGDFFPATLQDNKRVTVVGTRTSGAGGYVKAINIPNLLGLERFTLT
                     GSIAERIDKNPIENLGVTPDVPLEMTIEDYRDGFKHYISEVKKVLTEQIRAF"
     gene            complement(1324..1614)
                     /locus_tag="VIG33_06995"
     CDS             complement(1324..1614)
                     /locus_tag="VIG33_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270620.1"
                     /translation="MNPQTLHCFNCNKEILSIVGRRDECIQCRADVHVCRNCEFYDPK
                     VYNECREPQADVVKEKERANFCDSFSPRKGGSGGLDKAAALRAAAEALFKKP"
     gene            complement(1622..2593)
                     /gene="glpX"
                     /locus_tag="VIG33_07000"
     CDS             complement(1622..2593)
                     /gene="glpX"
                     /locus_tag="VIG33_07000"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEY8270621.1"
                     /translation="MDRNLALEFVRITEAAALACAEWTGHGDEKAADKAAVDAMRKAF
                     DSVNIDGTVVIGEGERDEAPMLYIGEKVGNKAFNAPAIDIALDPLEGTTICATGGVGS
                     VSVIAVAERGNFLHAPDTYMDKIACGPGAKGCIDIDKSATENVKRVAEALKKTVGDLT
                     VVILDRPRHEALIAEVRKTGARIHLIGDGDVSAAIATGWADSGIDLLMGIGGAPEGVI
                     SAAAMQCLGGDFQGRLKFRNEHEKERATAMGIKDLTKKYTVDELAKGSVMFVATGVTD
                     GPFLKGVKTLPGRQAKTHSVVMRSVTGTIRTIEAHHKLSQKPNYKTI"
     gene            complement(2572..2997)
                     /locus_tag="VIG33_07005"
     CDS             complement(2572..2997)
                     /locus_tag="VIG33_07005"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY8270622.1"
                     /translation="MVSLSRMYVYQHRVQYYETDEMGIVHHSNYLRFCEEARVAWAHS
                     RGLLDYQKKGSASLFAVYETQVRHLKPAFFGDELEVQLEVKAEGVRIYIQYRLNARGQ
                     TLAVAETTHVSLDQNLKLMRLPSDMMQILENEKWTETWL"
     gene            3138..3737
                     /gene="pgsA"
                     /locus_tag="VIG33_07010"
     CDS             3138..3737
                     /gene="pgsA"
                     /locus_tag="VIG33_07010"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY8270623.1"
                     /translation="MNTGTPINTLSGAPPSWKMQLPMWITVSRIAMVPFILICMHFNI
                     FWWNVAAASLFMLASITDYYDGYFARKFQAVSNMGKFMDPVADKILVSSILVMMTYNQ
                     KIDPWMVVAILGRDNFISGVRAVAATDGLVIAAKSAGKWKTALQMGAIPGLLIGEIPG
                     YLPYLDKIAYIILWISVLLSISSGIEYYLAYKRQRKAKG"
     gene            complement(3837..4025)
                     /locus_tag="VIG33_07015"
     CDS             complement(3837..4025)
                     /locus_tag="VIG33_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270624.1"
                     /translation="MRGIFKKSLIKWSAIASLGISAYFLGYFWGVKNLGSEAKSPMSA
                     SEVSDRAADLSKLKERVR"
     gene            4092..4167
                     /locus_tag="VIG33_07020"
     tRNA            4092..4167
                     /locus_tag="VIG33_07020"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4125..4127,aa:Gly,seq:gcc)
     gene            4222..4581
                     /locus_tag="VIG33_07025"
     CDS             4222..4581
                     /locus_tag="VIG33_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HEY8270625.1"
                     /translation="MAVSVQELTKALESLRIALESPYSDIVRDASIQRFEFCIELCWK
                     LLKKKMGTSSVAPKAVLREAAAQGLIANIEIWFDFIEARNLTSHTYNESVAKTVFETV
                     QRFFPEATQLLTKLNSI"
     gene            4578..4877
                     /locus_tag="VIG33_07030"
     CDS             4578..4877
                     /locus_tag="VIG33_07030"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY8270626.1"
                     /translation="MKFGLKKEHWELVQLKLLAPLKQQKSKVWIFGSRARGDFKEFSD
                     LDVLYQAEVPLPPGLLSRIKEDLEESRLPIKVDLVAKEELASSYLENVSKDLIEI"
     gene            5120..5830
                     /locus_tag="VIG33_07035"
     CDS             5120..5830
                     /locus_tag="VIG33_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8270627.1"
                     /translation="MKFYYLVAAYNEERVLEKTVRELAVIPQRFPGSEILLLDNGSQD
                     NTWALCQRLAQENSWVSAYHNDEKGMGTAFKRGLRELQNKNIPRDSWIVFCASDLPFG
                     FSDLESFLELGPAAWQENILFVGSKRHSQSQVQRNWKRRFGSVIFELARFLILQIKTK
                     DTQGSLFLRADQIALLQNLNSNDYFFTVELVYFAEKSGKVTEMPIKLRPEIRNSNISI
                     LKDGSKSLLQLLKFRQRL"
     gene            complement(5827..6534)
                     /locus_tag="VIG33_07040"
     CDS             complement(5827..6534)
                     /locus_tag="VIG33_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270628.1"
                     /translation="MSNLIHSTAIIDPRAHIGTDNYIGPFCYLGPNVIIGDGNRFEAY
                     VSVGTAAEHREYFHQEAGPVKIGHKNIFREFVTINGATKGLTEVGDNVVMLRGSHLGH
                     DAVLGHKANLSCNVLIGGHTIIGEGANLGLGAAIHQFRVIGAYAMVGMNSTVTRHISP
                     FVVAFGSPCEGQKVNRIGLQRSGIPDIDLSIFENWFFQTKDHLESPDSIQHIYQRYID
                     KFAKDCGEQSRVLKKSA"
     gene            complement(6606..7619)
                     /locus_tag="VIG33_07045"
     CDS             complement(6606..7619)
                     /locus_tag="VIG33_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY8270629.1"
                     /translation="MSKSPPGNNEKKMVKVSKELFTDGLPLASEVFTRLPSGQFILIG
                     KKADTGNLSNLHLMKQGGEAELFVHEEDYSNVVRYNINLIEKTLKNESVSDTLKMNLL
                     KGVAESTISELVKKDVYVGSYDHCKQIVGFVQQTVAQVKDFDKFIEIFSKLPGDLVSH
                     SLATSVISLLICEHMNITMKSTLEKVVMGAFVHDIGLKEIPLEILRRPRLYWTEAEVH
                     LYEAHPLRGVEMLRDIKEIPSDVLAIVLEHHENALGLGYPRHIRDIKINPLARIVAIA
                     DCFVDFIYGTGVDVRNRTPEEAIGYIETTLGQPFNKPAFLALKKITHLTHLQKKLRVG
                     SRF"
     gene            complement(7706..8725)
                     /locus_tag="VIG33_07050"
     CDS             complement(7706..8725)
                     /locus_tag="VIG33_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY8270630.1"
                     /translation="MNLKYILGLGALLAGLVACSPETPKAYLSPVVPNEKVIEDNTQV
                     FDPKVDILFVVDNSGSMEAHQTNLARNVAQFTSTFTKSSALDYNIGIVTTEMPDMWGS
                     PSRVCCGQLVGSPRIVNKNTLGADQILARNFLVGTNGSSSEASFDPVVAALSSPLLNG
                     WNVGFLRPDASLVVILMTDAEDQSTRTDALGLYNFLLNLKHKDASKILAYGVLAPTNN
                     PQRCTRDEPFGSPLRIEKFLSMVQNNKNNIMSICDPDYGSRLATMAKDIVDKVGNIIY
                     LSRPPDFSSIRVSYGSVDLPEDYHKGWSFDPKMNAILLGDQINWSSQPSGSRIKVFYN
                     AAKFD"
     gene            complement(8745..10385)
                     /locus_tag="VIG33_07055"
     CDS             complement(8745..10385)
                     /locus_tag="VIG33_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270631.1"
                     /translation="MKLKHLAIVGLSWSLVPVAWAGDLESILDEGSKATKTLQAPAEA
                     GKTTLLAQAMKKIIGTPKSEQSIFLRYLEASDWEKATLQFPNAFEGTAFQKSANGRAL
                     FGYIQFQAGLPVTGVQTLFMAANPREINTMLREEWQKVAPADHFVWSLAQVQWQSAWE
                     VIFGEIIPLRLKIMEAFVSEKNSEKNSEKNSEKNIDQLQKLSSMAPANSKEKAQIDWQ
                     LVIAYSLSDQTDRAAKLLANLMKSPYVTVSKDLMQITAARLLFQGGYFDTSLKYYGKV
                     SKTSDYWTEAQEEMGWAYIRKGETGNAMAVSQSLVNSGLNFQVSPEAFFVRALSLLKV
                     CDYSGVTETLQNFPKRFKERTKTLEALSADPANQDVDKGIALLKTKKIEVQELGKTAQ
                     NLPRRFAHDDRLYQFAQVQKHLEDEAQAAEKLYAKSLSLTGLQGSFEKLKQNTLQRAQ
                     MAKTSAYSRVKELAKEEVLETKEILHKLHIVEAEVIQQVSLADKIAKNAQGNDEKKGS
                     TGYKGTANAVKFPAEDEVWFDELSNYRIDVKKACTVKK"
     gene            complement(10382..11116)
                     /locus_tag="VIG33_07060"
     CDS             complement(10382..11116)
                     /locus_tag="VIG33_07060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HEY8270632.1"
                     /translation="MKRITLLLSLLFAVSAMAANQEGAKEESTEAEFDALGGNKILLD
                     KAKALEPEKSISIVQNRTVSLENRVEIAPEFSGTFGGDTYTRTKSIGLNAFYHFNPRW
                     AIGAKYNYSFNSMTPEGESLVNQAYEQFKKDPANPTLGYPQMDYPKSETMALFNYSPI
                     YGKLNLFDKSIAHFDVYLLGGYGQVALSSGGASTYTAGGGLGFWVSRHFSTRFEMRYQ
                     SYTAKYFETQKKMDLAVASVQMGWLL"
     gene            complement(11116..12363)
                     /locus_tag="VIG33_07065"
     CDS             complement(11116..12363)
                     /locus_tag="VIG33_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270633.1"
                     /translation="MKNIKYFLNTTLNIISSVILSSTLVVGILAGCASQWNQDPLADK
                     DGVLKTGQPKPTQPEERKAIDSNNVVVDSPELYAFREGTLGELVVTARILENGYAAAM
                     SVENLADFPGANFAVVNYDSATRIQTAKFTWTPAFGVVSGNSGLDEKKDLKLLILGSK
                     PNSKVIVGHRSVKVSIGKMLNVPEIFSISQTSISLREGEKATLNLKIRDKDAGIDVKS
                     YPNLLILPTVGYADPSQFVTINQIVATGSGEFTVFLGIDLTSAELTKSRGIFSFNLKA
                     VSRFNQVSSAQSVLLNIVTSFSDLQSSWYDVLETPAGSKTTFQFMIFDPKTELSVSNP
                     NFSGLPSNATVNCSAVGTNQLCTFSWTPDLSVPAGDYSFQAQVTGRSQDPQDTFVKNN
                     SFNLKLRVTSAPPVSPLALQGGK"
     gene            complement(12365..13498)
                     /locus_tag="VIG33_07070"
     CDS             complement(12365..13498)
                     /locus_tag="VIG33_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY8270634.1"
                     /translation="MDKQLVLEDHEGTVLRAFNWKGDTGEVIRRGDTRRLEVVRTTDH
                     LSKNKIPFERIGQVSKQGLEKAPFLVGKLGHLKLVEEVGETLKDKVRENESERRWYSI
                     LAAVLILATLFMTYIHSLPTMTDKMEQNLKQEVVKIIKNIPPKPKVEMKTVAEKKQEV
                     TPPKTVTKTTTTLKRMGALAVLGSLSKGKQKGGVDLGAVNTSAGPGLGGTQGSGGVQT
                     SLYGKGVVAAPVGVGGNIQGAGGYGTKGKGGGHAGYGKLSLIGSAGTSSIALTQEASV
                     ASGLDRDQIAAVINRNLGQVRFCYEQGLQGDPNLNGRVAIAFVIGGNGAVKMASVDNS
                     TMNAKAIEDCILMRLKTWQFPLPQGGVDVKVTYPFVLRRAGQG"
     gene            complement(13500..13775)
                     /locus_tag="VIG33_07075"
     CDS             complement(13500..13775)
                     /locus_tag="VIG33_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270635.1"
                     /translation="MKSIVLILSLLATVALAVPSKETKNRSSESAVSTQKNSEATNYN
                     FEDLLVQGKYNFSDEAVTTVEEDKVLDSLIGVRSDFKDRLKSSTGSY"
     gene            complement(13772..16789)
                     /locus_tag="VIG33_07080"
     CDS             complement(13772..16789)
                     /locus_tag="VIG33_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270636.1"
                     /translation="MLKKVLVAQSLLQSFRQAWMKVCLRNCALISILIPLSFSQAQMD
                     EGNRNLLIDKLEKVYQHLAPKDSSKVAVTLRLADLYAERARLESMKEIDQGCTVCKVG
                     DLDRKKALRLYTEVLARAPEAIQGKVMVQVGHLYQLTGDEAKAITFYNKILQEGAPSE
                     LKAEAQISLAEIYFKSREFAKAQGFYQDFLKNATAGSRGLAAYRSAWCAFNLGDINSS
                     LKQIEQILRTPSLLARNGSSQTQVDPQFQEEVSRDYATFLAKKTVENKDIENLFQLSP
                     ETIRVQNVKSLALDLERIGKKADSLKVWTFVFGYMNKPEDRLAAKISMAQLYFDTANK
                     KEGAESFESAMTLWKDLKACQSNQCEELRRRSRQFVVSWNQLEKKSPTAELLAAYQVY
                     LKNFPEDAEMNLYAATVAREIKNWNAAWEHYNKALAIQLGDAKNDPKLAEKLETILVA
                     MLDVAENSKNEKFVTEIYEVYLAQSIKKTKAFEVLYQKAHRLYEQGQYEAASQELRKL
                     ALDKTGDAKIRKQAADLSLDALVLMKDESRISSWAAEYAQVLTEGSKEFSQIVQKAVL
                     TKSANVADKDSATAYAQLGEFNPAQATAEDKIKFYKNKLILAEKLNKFLEAQAAAESL
                     LQQSGLSAEDAEYAWARKAYIAEMRLDFASVLTATHKLQKSLKPDEKMLKLAMFADLS
                     GQKSSVYYDQYLQTSKDEENKKLVVTELVRKSKAPEKEIDLHHALLSKYPQLMAQLYT
                     EVFAKTSSETILKKVIKDPAMKATDPGKLLTRISFLRDFSAFKKSLVAHKLDTKSDRK
                     LAATMKARVALLEKLENFTKQAIETGDWTSQLVSLDALAKESERFYSELLSAPIPPSL
                     SDEEQQQYMQLLSAQATPFQAKSVEAKSKVEQFWKSSNWHSSLKASWQQADLRSIIVT
                     EVNALREIAPSDYQTELAELVIVKKDSTAIAARPSLKELQMAMAQVQQNPFDKQALEA
                     LLELEKKSENSAMSQYLQTRIENLAKSSEKGVL"
     gene            complement(16817..17317)
                     /locus_tag="VIG33_07085"
     CDS             complement(16817..17317)
                     /locus_tag="VIG33_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY8270637.1"
                     /translation="MLANQGVLHETALQSMIESQSLINPKHGKWKRNIVADLLLTALI
                     DAFSILVIFLLMSFSSTGDMLYISKGQELPKASLADILERNPVVKLEEGKIFLESKEV
                     TQNSLVGALLDMRKQFIESHPGEEAPNILMVQGDRRLKYEQLNQIILAASHAGYSDIR
                     FAVMMK"
     gene            complement(17330..17785)
                     /locus_tag="VIG33_07090"
     CDS             complement(17330..17785)
                     /locus_tag="VIG33_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY8270638.1"
                     /translation="MRKHGKHLDFEINLIPCIDLLSVCICFLLLTAVWLHVGSMNVKQ
                     AVGGQSQAETPKKPLLWVYMGQDGQLSFNVQQSSLVPAALVNLKLPGIEGHANFSELN
                     RILTQLTTKDPDLKTALIQPQAQSSYEEIIALMDEFKKLGLTDLGIAPL"
     gene            complement(17797..18510)
                     /locus_tag="VIG33_07095"
     CDS             complement(17797..18510)
                     /locus_tag="VIG33_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY8270639.1"
                     /translation="MDLLTKIALAFEHGGFWMWPILAIQITSIAIMIERTYALFIKRK
                     LDQHTVAAGFEDNVRRGELDIVIQKARAQEQTSPVARAVVAGASAAKFLGGKEEIQGK
                     MDEVLIAENALLDQRTGFLSMLGNVATLTGLLGTITGMIKSFAAVAYANPSEKAALLA
                     SGISEAMNATAFGLISAIPALVAYAILQNRANRLADDLNHSALKVFNWLSFSYEPVGF
                     KSFKGASMHSEKITAELNS"
     gene            18685..19500
                     /locus_tag="VIG33_07100"
     CDS             18685..19500
                     /locus_tag="VIG33_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8270640.1"
                     /translation="MYEFILLFCSLIAGFLGSILGLGGGLIIVPTLTLLFHVNIRYAI
                     AASLISIVGTSSGAAASYLKDHFANLRIAVLLELGTVTGAVSGFLIAKKIQASFLFLL
                     FGVFLFFSAVMMLRSRDEHTSTKNHPWSEKLNLAGSFPDGKGGVLPYNVDNVPFGLTA
                     MYAAGVISALLGIGSGIFKVLTMDRAMKLPMKASSATSNFMIGVTATASAGAFLLRGD
                     IRPEIAAPVSVGIILGSWMGAHSMIKIPTQILRKIFVIVLSIASFQMILKGLS"
     gene            19497..19904
                     /locus_tag="VIG33_07105"
     CDS             19497..19904
                     /locus_tag="VIG33_07105"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HEY8270641.1"
                     /translation="MNTQDLPEDLFIKDPLKDPLLNLELRISKLLHWGVSCAEALMLL
                     GWITFFDFSENPLLKFQDYKEESLQKSLQSALQDHHWGLLLAYAGLVLLISLPILRVL
                     MTGIFFAKQRNQILALASFFVFVILLFSFTLGI"
     gene            complement(19932..20375)
                     /locus_tag="VIG33_07110"
     CDS             complement(19932..20375)
                     /locus_tag="VIG33_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270642.1"
                     /translation="MRLIRGLFGAGFGVLLISFKAGALPQASDFKEVGEEIQEVQEVS
                     KDEERRPQLGENEKILSGTVRIIRKLEMTEVFFKDLNDSYYIPSGRGYSAIYKALEES
                     SKKGTPVSFKANTKNRRVLSLESAPLKSPASVTQEKSTLSPNGSK"
     gene            20477..>20850
                     /locus_tag="VIG33_07115"
     CDS             20477..>20850
                     /locus_tag="VIG33_07115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8270643.1"
                     /translation="MRILHLLSQMQPTGAETYAVTLANWLTDQGHDVFIISDRIHVKT
                     RQPYIPLAVHDKRLPTRIKSTFFLRKFLREHNIQIIHCHSRAAARLAYWATQGTKVAV
                     VSTIHGRQPISLSKKLLNIYGE"
BASE COUNT         5661 a         5008 c         4218 g         5963 t
ORIGIN      
        1 ctgccacaag tttcccttac aaggtaggcg atgaagtctt ggcaatggat gaaattcctg
       61 taaatcagct aaggtcaagc ttaaccaagg acatgaaatc gacagcaccc ttaactgatc
      121 aggcgcttgg tgatatgttc ttgaccagca gaaggggcca aagaaaccag ctcgttcctc
      181 gcaatacagt cactttaacc cttctgagag ctggcgaaag cattcctcga acgcatcaat
      241 taaattggga atatacagct gaacaaattc caggggcaga aacgctgccc tctcaaaatc
      301 agttcaataa gcagctcagt tttcatggac attttaaatt agcgcaagcg cagatgttaa
      361 gtcccgttgc tgatatgttc gcgcagcagg ataattttat agattcaccc tacggcctgg
      421 gaaataaaaa accattttta ccagactttg gccctcgcgt ttgggaaacg gacgccacca
      481 atacttttga tgcatacatc taccaaaatg aaacgagtca actgattgga gtgatcagga
      541 tagccagcta ttcccccaat agcgaggacc ctaaaggcag agaaaaagca gtggctgatt
      601 tttctgagat ccttaagaaa atgcaaaaat taacttcagg cttagtgatt gatcaaacga
      661 acaacccggg tgggtcgata ttttatctct actctttagt atccatgtta actgaccaag
      721 ctttttatac tccaaaacat agaatgagta ttgataccat ggaggtgaag ccctgtgttg
      781 agtttgaaaa ggcttttgag aaaatttcta acgatgaaga ggcaagaaaa ttgctaggag
      841 agtccgatga cggattcccg gtaaattacg aatacgctgt ggccgcaaaa aattactgcc
      901 aatttatggt aactgaattt aaacagggaa agaaaatcac ggagcctttt tatatttttg
      961 gagtcgacaa agtgaatcca agccccgtgg cgcagtatct taaacccata gttattttgg
     1021 tcaacgaact cgatttttct ggaggagatt tctttccagc aactctgcaa gataacaaac
     1081 gagtgaccgt tgtaggaacc agaacctcgg gtgctggcgg ttatgttaaa gccataaata
     1141 ttccgaacct attagggctt gaacgtttta ctcttacagg ctctattgcc gagcgaattg
     1201 ataaaaatcc tatcgagaac ctaggagtaa ctcccgacgt gccacttgag atgacgattg
     1261 aagattacag agatggcttc aaacactaca tttcagaagt taaaaaagtt ttaactgaac
     1321 aaattagggc tttttaaaaa gagcttcggc ggccgctctc agcgccgccg ctttatccag
     1381 accaccgctg ccgcctttgc gaggcgaaaa ggaatcgcaa aaattagcgc gctctttttc
     1441 tttcaccaca tccgcttgag gctcacggca ttcattataa actttagggt catagaattc
     1501 acagtttcta caaacatgga catcagcacg gcactgaata cactcatcgc ggcgcccgac
     1561 gatgctcaaa atttctttat tgcaattaaa acaatgtaag gtctgtggat tcataattat
     1621 tttatattgt tttgtaattc ggtttttgtg aaagcttatg atgagcttca attgtgcgaa
     1681 tggttcctgt cacggaacgc atcaccacgg agtgagtctt ggcctgacga cctggaagag
     1741 tctttacgcc ctttaaaaaa ggaccatccg tcacacccgt ggccacgaac atcacagatc
     1801 ccttggcaag ttcatccaca gtgtattttt ttgtaaggtc tttaatgccc attgcagtgg
     1861 cacgttcttt ttcatgctca ttgcggaatt tcaaacgtcc ttgaaaatcc ccgccaagac
     1921 actgcatcgc tgccgcggaa ataacgcctt ccggagctcc gccgataccc atcagtaaat
     1981 cgatgcctga atccgcccag cctgtggcaa tcgcggcaga gacatcccca tccccgatca
     2041 agtgaatgcg agcccctgtt ttacgcactt ccgcaatcaa agcttcgtga cgtggacggt
     2101 ccaaaatgac caccgtcaaa tcgcctacgg tttttttcaa agcctcagcc acgcgcttta
     2161 cattttccgt cgctgattta tcaatatcga tacaaccttt agcccctgga ccacaggcga
     2221 ttttatccat ataggtatca ggagcgtgca agaagttgcc tctttcagcc accgcaatca
     2281 cggacactga gcccacgccg cctgtagcac aaatcgtcgt gccttccaag ggatccaaag
     2341 caatatcgat tgccggagca ttaaaggctt tgttaccgac tttttcgccg atataaagca
     2401 tcggagcctc atcgcgctcg ccctcaccaa tgacgactgt tccgtcgatg ttcacgctat
     2461 caaatgcttt tctcatggcg tcaacagcgg ctttatccgc agctttctca tcgccatgac
     2521 cggtccattc agcacaagcc aaagcggctg cttccgtaat acgcacaaac tctaaagcca
     2581 ggtttctgtc catttttcgt tctccaggat ttgcatcatg tcgcttggta agcgcattaa
     2641 ttttaggttt tgatctaagg aaacatgggt cgtttcggcc actgccaaag tttgcccacg
     2701 ggcgttcaga cgatattgta tgtagattcg cacgccctca gcctttactt caagttgaac
     2761 ttcgagttcg tctccgaaaa aggcgggctt taaatggcgc acttgggttt cataaactgc
     2821 aaaaaggctg gcagagccct tcttttggta atccagcaac ccacgggaat gagcccaagc
     2881 cacacgggct tcctcacaaa aacgcaggta attgctatga tgaacaatcc ccatctcgtc
     2941 ggtttcataa tactgaacgc ggtgttgata aacatacata cgacttagcg ataccatcac
     3001 aaaaaccttc ttagaaagca aaacgtttat tcaaatttta actaatattt taaactcttt
     3061 tctttttatt taccgttgtt gacagtgata aagacgtttt gagaagatca ttcgagatca
     3121 actaaaggac tgatattgtg aacacaggca cacctatcaa cacgctgagt ggggcaccgc
     3181 cttcgtggaa aatgcagctt ccgatgtgga ttacggtcag tcgcattgca atggttccct
     3241 ttatcctgat ctgcatgcac ttcaacattt tttggtggaa tgtcgccgcc gcctctttgt
     3301 tcatgctcgc gtccatcacc gattactatg atggatattt tgcccgaaaa ttccaggctg
     3361 tttccaatat ggggaaattt atggaccccg tagcagataa gatcctggtc agcagcattc
     3421 tagtgatgat gacctataac caaaaaatcg acccctggat ggtcgtagcc attttggggc
     3481 gggacaattt tatcagcgga gttcgcgccg ttgccgccac agatggactc gtcattgccg
     3541 ccaaatctgc cggaaaatgg aaaacagcct tacaaatggg ggctatccct ggcttgttga
     3601 ttggagaaat tccagggtat ttgccttatc tcgacaaaat tgcttacatc atcttgtgga
     3661 tcagcgttct gttaagtata agtagtggca tcgaatatta tttggcttat aagaggcagc
     3721 gcaaagcaaa gggttgagtt atgttactgc atatgtcgcc aagagattgg ttttttattt
     3781 tcctggctgt tttactggct agttatgtaa aattggcttc tgccaaagcg cagctttcaa
     3841 cgaacccgct ctttaagctt actaagatca gccgctcgat ctgaaacttc agaggcgctc
     3901 atgggtgatt ttgcctcact ccccaggttt ttcactcccc agaaatagcc caaaaaataa
     3961 gcgctaattc ctaaagaggc gatcgcagac catttgataa gacttttttt aaaaattcct
     4021 cgcatgaaaa atatttaccc attgacagaa acagcacgca actgtaattt ctgtcacacg
     4081 ttcgttatga cgcgggaata gctcagttga tagagcgatg ccttgccaag gcataggtcg
     4141 cgggtttgag tcccgtttcc cgctccaaat ctctaaaaaa accagtcctg cgagactggt
     4201 ttttttattt ctggaggccc catggcggtg agtgttcaag aactgacaaa agctttagag
     4261 tcgcttcgca ttgccttaga atctccctat tcagatattg ttcgggacgc ctcaatccaa
     4321 cgttttgagt tttgcatcga actctgctgg aagcttttaa aaaagaaaat gggaacatcc
     4381 tcagtcgctc cgaaggccgt tcttcgtgaa gccgccgcgc aaggacttat tgcgaatata
     4441 gaaatctggt ttgatttcat tgaagcccgc aacctcacgt cccataccta caatgaatcc
     4501 gtggccaaaa ctgtttttga aacagtccaa cgcttctttc cggaggccac acaacttctt
     4561 acaaaattga actcaatatg aagtttggtc ttaaaaaaga gcactgggaa cttgtccaat
     4621 taaaattact tgccccactc aaacaacaaa aatcaaaggt ttggattttt ggctcgagag
     4681 cccgaggcga tttcaaggaa ttctcagacc ttgatgtcct ttaccaagct gaagtccccc
     4741 tgcctcctgg actcctcagt cgcatcaaag aggatcttga agaaagccgt ttgccaatca
     4801 aggtggacct tgtggctaag gaggaactgg cctcttccta cttggaaaat gtgagtaaag
     4861 atctcattga aatttaaaca gcgccgcaag catacaacat cttaaaaaga aattgatcgc
     4921 agaatccgcg aaaagtagaa agcattgatt tttaagtcaa tgcttttcga gatagggatt
     4981 gatatttcgt accggagaaa cgcgatcctc tttttggata ccctagcgtt gcgggaaatt
     5041 gtgttcgaaa tccaagaagt tcattgaccc acgtctgggt catcttgctc cttccgaaag
     5101 gaaggagtaa atttactcaa tgaagtttta ctatctcgta gccgcctata atgaagaacg
     5161 cgtccttgag aaaacagtgc gagaacttgc ggtcattccc caaagatttc ccgggtctga
     5221 aattcttctt ttagacaatg ggagccagga caatacttgg gccctttgcc aacggctggc
     5281 tcaagaaaac tcctgggttt ccgcctatca taacgatgaa aaaggaatgg gcaccgcgtt
     5341 taaaagagga cttcgggaac tgcaaaataa aaatattccg cgcgatagct ggatcgtatt
     5401 ttgtgcctca gacttgccct ttggctttag tgatctggag tcttttttag aattgggacc
     5461 ggcggcttgg caagaaaata tcctcttcgt cggcagcaaa cgtcattctc aatcccaggt
     5521 tcaaaggaat tggaaaagac gcttcggctc tgtcatcttt gagttggccc gctttcttat
     5581 tttgcaaatt aaaacgaaag acacccaagg ctctcttttt ctaagagctg accaaatcgc
     5641 tttgttgcag aatctaaatt ctaatgatta tttttttacg gttgaactgg tttattttgc
     5701 cgagaaatcc ggaaaggtca ctgaaatgcc catcaagtta cgaccggaaa tacgcaattc
     5761 caatatttca attttaaaag atggctctaa aagcctgcta caacttctta aattccgtca
     5821 gcgtctttaa gctgattttt tcaagaccct tgattgttca ccacaatcct tggcgaattt
     5881 atcaatgtag cgctgataga tgtgctggat ggagtctgga ctctcaagat gatctttcgt
     5941 ttgaaaaaac caattttcaa aaatggataa atcgatgtcg ggaatccccg atcgttgcaa
     6001 ccctatacga tttacttttt gaccttcaca aggactcccg aaggccacca caaagggtga
     6061 aatatgacga gtcacagtgg aattcatgcc caccatagca taggccccaa tcactcgaaa
     6121 ctgatgaata gcagcgccca accctagatt cgctccttca ccaataatag tgtgcccccc
     6181 aatgagaaca ttgcaggaaa gattcgcctt gtgaccaagg acggcatcat gaccaagatg
     6241 agaaccccga agcatcacga cattgtcccc cacttcagtg agacccttcg tcgctccgtt
     6301 gatcgtcaca aattctctaa aaatattttt atgaccaatt ttgacggggc cggcttcttg
     6361 gtggaagtac tcacgatgtt cagcggccgt gcccacagaa acataggctt cgaatcgatt
     6421 gccatctccg attatcacgt tgggccccag gtaacagaac ggaccaatat aattatccgt
     6481 gcctatgtgg gctctaggat cgatgatcgc tgtagaatga atgaggttac tcacacgaat
     6541 ctcctgccat tattttcgac acaagctcgt attttctaaa cagactttcg aagggttcac
     6601 cacacctaaa acctagaccc gacccttaat ttcttttgga gatgagtaag atgggtgatt
     6661 tttttaaggg ctaagaaagc aggtttatta aatggctgcc ccagagtggt ttcaatataa
     6721 ccaatagcct cttccggggt tctgttgcgg acatcgaccc ctgttccata gatgaaatcc
     6781 acgaaacaat ccgcgatcgc cacgatgcgg gcgagcggat taattttaat atcgcgaata
     6841 tgacgagggt aacctagacc caaagcgttt tcatggtgct ccagcacgat agcgagaaca
     6901 tccgaaggaa tctctttgat atcacgaagc atctccactc ctctgagagg atgagcttca
     6961 tagagatgaa cttcagcctc agtccaatac aaacgaggcc tccgcaatat ctccagagga
     7021 atttctttca gaccaatatc atgtacaaag gcacccatga ccactttttc taaggtgctc
     7081 ttcatagtga tattcatatg ttcacagatt aaaagtgata taacagaagt cgccagagaa
     7141 tgacttacga gatccccagg taatttgctg aagatctcta taaacttatc aaaatctttc
     7201 acttgagcca ctgtctgctg gacgaatccg acgatttgtt tgcaatgatc ataggatccg
     7261 acataaacat cttttttaac cagttcagaa atcgtcgact ctgcaacccc ttttagaaga
     7321 ttcatcttca aggtatcact cacactttca ttcttaagcg tcttctctat cagattgata
     7381 ttgtatcgaa ccacattaga ataatcttct tcgtggacaa agagctctgc ttccccgccc
     7441 tgcttcatga gatgaagatt ggaaagattc ccagtatctg cctttttacc aatgagaatg
     7501 aactgtccag acggaagacg ggtaaaaact tcggaagcta agggcaaacc gtcagtaaac
     7561 aactctttac tgactttaac catcttcttt tcattattac ccggagggga tttactcatg
     7621 aagtacttat cggtttattt cgatttcact tcataacatt ttcatataag ccaactatgc
     7681 gttagaatga gacgaaaagt ggtctctaat cgaacttagc cgcattgtaa aacaccttaa
     7741 tccgacttcc tgaaggctga gaactccaat taatttgatc ccccagaagg atcgcattca
     7801 tcttagggtc aaaagaccaa cctttatggt aatcctctgg aagatctacg ctgccataac
     7861 ttacgcgaat gctgctgaaa tctggaggac gagataaata gataatgttt cctactttat
     7921 ctacgatatc ttttgccatg gtcgcaagac gggacccata gtctggatca caaatactca
     7981 taatattatt tttattattt tggaccatgg agagaaattt ctctatgcgc aggggcgaac
     8041 caaagggctc atcacgagtg catcgctgcg gattgtttgt tggagccaat actccatagg
     8101 ctaaaatctt actggcgtct ttatgcttca aattcaataa gaaattataa agtcccaaag
     8161 catcagttct ggtgctctga tcctcagcgt ccgtcataag aataacaact aaggaagcat
     8221 ctggacgaag aaaacctacg ttccaaccat tgagcaaggg agaggaaagc gctgcaacca
     8281 ctggatcaaa gctagcttca cttgaagacc cgttggttcc caccaaaaag tttcttgcaa
     8341 gaatttgatc ggctccgagg gtatttttat tgacgatgcg agggctcccc accagttgac
     8401 cgcagcacac tctagagggg gatccccaca tatctggcat ttccgtggtg acaattccaa
     8461 tgttgtaatc cagagccgaa gacttggtaa aagtcgaggt gaattgggcc acattcctcg
     8521 ccaagtttgt ttgatgggcc tccatacttc cagagttgtc gacaacgaac aggatatcca
     8581 ctttcggatc aaaaacctga gtattatctt caatcacttt ttcattgggg actaccggcg
     8641 aaagataagc cttaggagtc tccggagagc aggctaccag cccagctagc aatgcaccta
     8701 aacctaaaat atatttaaga ttcacaatct acccctctcg atagctattt ctttaccgtg
     8761 caagcttttt taacatctat acgataattg ctaagctcat caaaccagac ttcatcttca
     8821 gccgggaatt ttaccgcgtt cgcagtgccc ttgtaaccag tacttccttt tttctcgtca
     8881 tttccttgag cattcttggc tattttatct gccaaggaaa cctgttgaat gacttcggcc
     8941 tcaacgatgt ggagcttatg aagaatctct ttcgtttcaa ggacctcttc ctttgccaac
     9001 tccttcaccc gagaataagc ggacgttttt gccatttgcg cccgttgcaa agtattttgt
     9061 ttcaattttt caaagcttcc ctgaagccct gtcagcgata gagactttgc ataaagtttt
     9121 tcagcagctt gagcctcatc ctcaagatgc ttttgcacct gggcaaactg atacaatcta
     9181 tcatcgtggg cgaatcgacg aggaagattt tgagcggtct taccaagctc ttgaacttcg
     9241 atttttttcg ttttcaaaag cgcaatgcct ttgtcaacat cctgattcgc cgggtcggct
     9301 gacaaagcct caagagtttt tgttctttct ttaaagcgct tggggaaatt ctgaagagtt
     9361 tctgtcaccc ctgaatagtc acacactttc aacaaagaaa gagctctcac gaagaaagcc
     9421 tctggactga cttgaaagtt aagccctgaa ttcaccaaag attgactcac agccatggca
     9481 ttaccggtct cgcccttgcg aatgtaagcc caacccatct cttcttgagc ctctgtccaa
     9541 taatcagagg ttttggacac tttcccgtag tatttcaaag aggtgtcgaa atagcccccc
     9601 tgaaaaagca atcgcgctgc ggtgatttgc atcaaatcct tactgactgt cacataggga
     9661 gacttcatca gatttgctag gagtttcgcc gcacggtcgg tttgatcact caaagagtaa
     9721 gcaatcacca actgccagtc gatttgtgcc ttttccttgc tatttgctgg agccatcgat
     9781 gacagctttt gcaattgatc tatatttttc tccgaatttt tctccgaatt tttctccgaa
     9841 tttttctccg agacgaaagc ttccatgatt ttcaaacgga gaggaataat ctctccgaaa
     9901 atcacttccc aagcgctctg ccactgaacc tgagccaagc tccatacaaa gtgatccgcg
     9961 ggagcaacct tctgccattc ctcacgaagc atcgtattaa tttctctcgg atttgcagcc
    10021 atgaaaagag tctgaactcc cgtcacagga agacctgctt gaaactgaat atatccaaac
    10081 aaagccctgc catttgctga cttttggaaa gctgtgcctt caaaagcatt ggggaattgc
    10141 aaagtcgctt tttcccaatc agaagcttca agatatctta aaaaaatgct ctgctcggat
    10201 tttggagttc caataatttt cttcattgct tgcgctaaaa gagtcgtttt tcccgcttcc
    10261 gccggagctt gaagagtttt ggtcgctttt gaaccttcat ccaagatgct ctcaagatct
    10321 ccagcccatg cgacgggaac caaagaccat gaaagaccca caattgccaa gtgtttcaat
    10381 ttcatagaag ccatcccatt tgcactgaag ccacggccag atccattttc ttttgagtct
    10441 caaagtattt cgccgtatag ctttggtagc gcatttcgaa acgggttgaa aaatgtcggc
    10501 tcacccagaa gcctagaccg ccaccggccg tataggtgga agctcctcca gaagagaggg
    10561 ccacttgtcc atagcctccc aaaagataga catcaaaatg agcaatgctt ttatcaaaca
    10621 agttcaattt tccataaatt ggagaatagt taaataaagc catcgtctca gactttggat
    10681 agtccatttg tggatagcca agggtcggat ttgctggatc ttttttaaat tgctcgtatg
    10741 cctgattcac caaggactcc ccttccggag tcatagagtt gaaagaatag ttgtacttgg
    10801 caccaatggc ccaacgagga ttgaaatggt aaaatgcatt gagaccaata cttttcgttc
    10861 tcgtataagt atccccaccg aaagttccgg agaattcagg agcaatttcc actcggttct
    10921 ctaaagaaac tgttctgttt tgaacaatac tgatgctttt ctcaggctca agagctttgg
    10981 ctttatctaa caagatctta ttgccaccaa gagcgtcaaa ctcagcttca gtgctttctt
    11041 cttttgcccc ctcttgattt gcagccatgg cagaaacagc aaataaaaga ctgagtagta
    11101 gagttattct tttcattatt tacctccttg aagggccaaa gggctcaccg gaggagcgga
    11161 agtcaccctc aacttcaaat taaagctatt gtttttaaca aaagtatcct ggggatcttg
    11221 gcttctgccc gtcacctgag cctggaatga atagtctccc gctggtactg acaaatctgg
    11281 agtccatgag aaagtacaaa gttgattggt tcccactgca ctacagttaa cagttgcatt
    11341 gcttggaaga ccagaaaaat ttgggttcga tactgacagt tcagtttttg ggtcgaagat
    11401 catgaattga aatgttgttt tagatcctgc tggagtttct aaaacatcat accaactgct
    11461 ttgcaggtca gagaaagaag tcacgatatt taacaataca ctctgcgcgg agctcacttg
    11521 attaaaacga gaaacggctt tcaaattgaa agagaaaatg cctcgagatt tagtcaattc
    11581 ggcacttgta agatcgattc ccaagaagac cgtgaactca ccacttcccg tcgccacaat
    11641 ttgattgata gttacaaatt gagaagggtc cgcatagccg acagtaggta aaatcagaag
    11701 gtttggatac gatttaacat cgatgccggc atctttatca cgtattttta gatttaaggt
    11761 cgccttctct ccctcacgca ggctgattga agtctgggaa attgagaaga tctcaggaac
    11821 attcaacatc tttccgatgc tgaccttgac tgaacgatgc ccaacgataa cttttgaatt
    11881 aggcttactc cctaatatta aaagtttgag gtcctttttt tcatcaagtc cgctgtttcc
    11941 actaacgaca ccgaaagcag gggtccacgt aaacttggct gtttgtatac gagtcgctga
    12001 atcataattg accacagcaa agttagctcc gggaaaatca gccaaattct ctacggacat
    12061 agctgcggca taaccattct ctaaaatgcg tgccgtgacc acaagctctc ccaatgttcc
    12121 ttcgcggaaa gcgtagagtt caggactatc aaccaccaca ttgttactat caatggcttt
    12181 tcgttcctct ggctgagtcg gctttggttg gccggttttc aaaactccgt ctttgtctgc
    12241 caaaggatcc tgattccatt gagaagcaca gcctgcgaga attccaacaa ccaaagttga
    12301 acttagtatt acgcttgata ttatgtttaa agtcgtattt aaaaaatatt taatgttttt
    12361 catattaccc ctgaccagcc cttcttagca caaatggata cgttactttc acatccactc
    12421 ctccttgcgg aagagggaat tgccaagtct tcaatctcat caaaatacaa tcttcaatcg
    12481 ctttggcatt catcgttgaa ttatcaacgc tggccatttt taccgcccca ttgccgccaa
    12541 tgacgaaagc aatggccaca cgaccgttta agtttggatc cccctgtaaa ccctgttcat
    12601 agcaaaagcg cacttgaccc agatttctat taatcaccgc cgcaatttga tcacgatcaa
    12661 gccctgaagc cacgcttgct tcttgagtca aagcgatcga actcgttccc gccgaaccga
    12721 taaggctgag ctttccatag cccgcatggc cgccaccttt acctttggtc ccgtaaccac
    12781 cggcaccctg gatgttgcca cccacgccca caggagctgc cacaacacct ttgccataga
    12841 gagaggtttg aaccccgcca cttccctggg tgccgccaag acctggaccc gcgctggtat
    12901 tcaccgcgcc gagatccact cccccttttt gtttaccttt tgaaagactt ccaagcacag
    12961 ccaaagcccc catacgtttt agagtagtgg ttgtctttgt cacggttttc ggtggagtga
    13021 cttcttgttt tttctcagct acagttttca tttccacctt tggttttggt ggaatatttt
    13081 taataatttt cacaacctct tgtttgaggt tctgttccat cttatctgtc atggtcggta
    13141 gagagtgaat ataagtcata aagagtgtcg ccaggattag gacggcggct agaatggaat
    13201 accagcgacg ctcgctttca ttttcgcgga ccttgtcttt caaggtttcg cccacctctt
    13261 caaccaattt caaatgaccg agtttgccga ctaagaaagg agctttctcc aaaccttgct
    13321 tgctgacttg tcctattctt tcaaaaggaa ttttattttt agaaagatgg tcagtcgttc
    13381 ggacgacttc aagacgacgt gtatcgccac ggcgaatcac ttcccctgtg tcacccttcc
    13441 aattgaaggc tcttaaaacg gtcccttcat gatcttctaa aaccagttgt ttatccatat
    13501 tagtaacttc ctgttgaact ttttaaccga tctttaaaat ctgaacgcac cccaatcaaa
    13561 gagtccaaga ctttgtcttc ttcaacagtg gttacagcct cgtccgaaaa attatactta
    13621 ccttgaacta aaaggtcttc gaagttgtag ttcgtagcct ctgagttctt ttgcgtggac
    13681 actgccgatt ctgaagatct atttttcgtc tcctttgacg gaaccgctaa cgctacggtc
    13741 gccaacaagg aaagaattaa aacgattgac ttcatagaac ccccttctca gaagacttcg
    13801 ccaaattttc aatacgtgtt tgtaaatact gagacatcgc cgaattctca gatttctttt
    13861 caagctccaa gagagcttcg agcgcctgtt tatcaaatgg attttgttga acctgagcca
    13921 ttgccatctg caactctttt aagctaggtc gggccgctat tgccgtagaa tcttttttaa
    13981 caatcactaa ctctgccagc tctgtttggt aatctgaagg cgcgatctca cgaagagcat
    14041 tcacttccgt aactatgata ctgcgaagat ctgcttgctg ccatgaggcc ttcaaagaag
    14101 aatgccagtt tgaacttttc cagaactgtt cgacttttga tttcgcttcc acagacttag
    14161 cctgaaaggg tgtcgcttga gctgacaaaa gttgcatata ctgttgctgt tcttcgtctg
    14221 aaagacttgg aggtatcggc gctgacaaaa gttcagaata aaaacgctca ctctctttag
    14281 ccagggcatc aagactgacc aactgagagg tccaatcccc ggtttcgatc gcttgcttcg
    14341 tgaagttctc taacttttca agaagtgcca ctcgcgcctt catagtcgct gccagcttac
    14401 gatcgctttt cgtatccagt ttatgcgcaa ccagagattt tttaaatgcg ctgaaatctc
    14461 tcaagaaaga aatgcgcgtt aagagtttcc cggggtctgt tgctttcata gctggatctt
    14521 tgataacctt tttcaggatg gtctcgctgg aagtcttggc aaaaacttcg gtgtaaagtt
    14581 gtgccatcaa ctgaggatac ttactaagaa gagcatgatg caagtcgatc tctttttcag
    14641 gagcttttga cttcctcacc aactcagtaa caacgagttt tttattttct tcatctttag
    14701 aggtttgcaa atactgatca taatacacag agcttttctg acctgaaaga tcagcgaaca
    14761 ttgccagttt taacattttc tcgtctggct tcagactttt ttgcaattta tgagtcgctg
    14821 tcaaaacaga agcaaagtcg agcctcatct cagcaatata agcttttctc gcccatgcat
    14881 attctgcatc ttccgcactc aaaccagact gctgcaacaa actctccgcc gcagcttgcg
    14941 cctctaaaaa cttgttcaat ttctcggcca agatcagttt gtttttataa aatttaattt
    15001 tatcttcagc ggttgcctga gcaggattga attcccccaa ttgtgcatag gctgtggcac
    15061 tgtctttatc cgccacattt gcagactttg taagaacggc tttttgaaca atttgtgaaa
    15121 actccttaga cccctcagtt aagacctgtg catactctgc cgcccagcta ctgatccgag
    15181 actcatcctt catcaacaca agagcatcca atgacagatc agccgcttgt ttgcgaatct
    15241 ttgcatcgcc tgttttatcc agagctagct ttctcagttc ttgagaagct gcctcgtact
    15301 gaccctgctc atacagacga tgagcttttt gatagagaac ttcaaaagcc ttcgtcttct
    15361 tgatactttg cgcgaggtag acctcataaa tctcagtgac aaacttttcg tttttggagt
    15421 tctccgcaac gtccaacata gccaccaaga tcgtttccaa cttttctgca agcttagggt
    15481 cattttttgc atcacccaac tgaatcgcta gagccttatt ataatgttcc caagctgcat
    15541 tccagttttt aatctctctg gctacggtcg ccgcataaag attcatttca gcatcttcgg
    15601 gaaaattttt aaggtaaact tgatacgcgg ccagaagttc cgccgtgggt gatttttttt
    15661 caagctgatt ccaagacacc acgaactgac gagaacgtcg gcgaagctct tcacactgat
    15721 tgctttgaca agctttgagg tctttccaca aagtcattgc ggattcaaaa ctctcggcgc
    15781 cttctttttt attggcagta tcaaaataaa gttgagccat agaaattttc gcggccaaac
    15841 gatcttccgg tttgttcatg tagccaaata cgaaagtcca aacctttaaa gagtccgctt
    15901 ttttgccaat tctctctaaa tcaagcgcca agctctttac gttttggact cgaatagtct
    15961 ccgggctaag ctgaaacaag ttttcgatgt ccttgttctc aacagtcttt ttcgctagga
    16021 aagtcgcata atctcgactc acctcttctt ggaattgagg atcgacttga gtctgactag
    16081 agccgttacg cgctaataag gagggggttc gcagaatttg ctcaatttgc ttaagcgaac
    16141 tattgatatc gcccaagtta aaagcacacc aggcactgcg ataagcggca agcccccggg
    16201 agcctgcagt cgcatttttc aaaaagtcct gatagaatcc ttgcgctttt gcaaactcgc
    16261 gacttttaaa ataaatctct gccaaagaga tttgagcttc ggctttgagt tcggaggggg
    16321 caccctcttg aagaatttta ttatagaaag tgatggcttt ggcttcgtcc cccgtcaact
    16381 gatacaaatg accgacctgc accatgacct tgccttgaat cgcttctgga gctcgagcca
    16441 agacttcagt ataaaggcga agagcttttt tacggtctag atctcccacc ttacaaactg
    16501 tacaaccctg atcaatttct ttcattgact ctaggcgcgc ccgttccgcg tacagatcag
    16561 ccaaacgaag ggtcacagcc actttcgaag aatctttggg ggccaaatgc tgatagactt
    16621 tctctaactt atcaatcagt aagttgcgat tgccttcgtc catttgcgcc tgagagaagc
    16681 ttaaaggaat taagatgcta atgagagcgc agtttcgcaa acaaactttc atccaagctt
    16741 gcctaaaact ttgcaacaga ctttgcgcta ctaaaacctt cttcaacata acaacctcac
    16801 ttaaggcttg tggtctttac ttcatcatga cagcaaagcg aatatcgcta taacccgcat
    16861 gagaggccgc aagaatgatt tgatttaact gctcatactt taagcgacga tcgccttgaa
    16921 ccattaatat atttggagcc tcttcacccg gatgactttc aataaattgc ttgcgcatat
    16981 ccaaaagtgc cccgaccaat gaattctgag taacttcttt agattccaga aaaatctttc
    17041 cttcttctaa tttcaccacg ggatttctct cgaggatatc cgctagagaa gccttcggaa
    17101 gctcttgacc tttgctaata taaagcatgt cacctgtact cgaaaaactc ataagaagaa
    17161 aaatgaccaa gatagagaac gcatcaatca aagccgttaa caaaagatcc gcaacaatgt
    17221 tgcgtttcca tttgccatgc ttgggattga tcaaggactg agactcaatc attgattgga
    17281 gagctgtttc gtgaagaaca ccttgattgg ctaacatgaa atcccccccc tacaatggag
    17341 ctatacccag atccgtcaaa ccgagttttt taaactcatc catcaaagca atgatttctt
    17401 cataggaaga ctgcgcctga ggttgaatta aagccgtttt caggtctgga tctttagtcg
    17461 ttaattgagt caaaatgcga ttcaattcag aaaagttcgc atgtccctca attcctggaa
    17521 gttttaaatt caccagagct gctggcacca aggaagattg ctgaacattg aaactcagct
    17581 gtccgtcctg tcccatatac acccataaaa gaggtttctt tggagtttcc gcctgagatt
    17641 gaccaccaac cgcttgtttg acgttcatgg atcctacatg gagccacact gctgtcagga
    17701 gcaaaaaaca aatgcacaca gacagcaagt cgatacatgg gatcaaattg atttcaaaat
    17761 ctaagtgttt accgtgcttg cgcataaaac accttcttag ctgttaagtt cagctgtgat
    17821 tttttctgag tgcatagatg cacctttgaa agatttaaag cccactggtt catatgaaaa
    17881 agacagccaa ttaaaaactt tcaatgcact atgattaaga tcatcggcca agcgattggc
    17941 gcgattttgt aaaattgcat aagctaccag agccggaatc gccgagatca atccaaaagc
    18001 tgttgcattc atcgcctcag agataccgga agcaagaagc gccgcctttt ctgaagggtt
    18061 cgcataagcg accgctgcga atgacttaat cataccagtg atggttccca gaagacccgt
    18121 taatgtcgcc acgtttccaa gcatagacaa gaatccagtg cgttgatcaa gaagcgcatt
    18181 ctcagcaata agcacctcat ccattttgcc ttggatctct tctttacccc ctaagaattt
    18241 ggcagcacta gcacccgcaa cgaccgcacg agctaccggt gaagtttgct cttgtgctct
    18301 tgctttttga atgacgatat caagttcgcc gcgacgtaca ttatcttcga atcccgcagc
    18361 cactgtatgc tgatccaact tacgcttaat aaaaagcgcg taggttcttt caatcataat
    18421 tgcgatcgaa gtgatttgaa ttgccagaat cggccacatc cagaatccgc cgtgttcaaa
    18481 agccaatgcg atttttgtaa gtagatccat gctgttcccc tcgttaagta tatggtctag
    18541 gacttaagga aaaggaatag agcaaatcgc gaagattgtg tcattatttt tttatccaat
    18601 ttaaaacaaa acggaaaatc gtcacaacat gttaggactt cttaaccatt taccctgctt
    18661 atttctagag ctaaaaataa acgcatgtat gaatttatac ttctgttttg ctccctaatt
    18721 gctggttttt taggctcaat acttggactt gggggaggcc taattattgt tccaacactg
    18781 actttgttgt ttcatgtcaa cattcgttat gcaatcgctg cgagtcttat ttccattgtt
    18841 gggacctcct caggcgctgc tgcaagttat cttaaagatc acttcgcaaa cctgcgcatc
    18901 gcggttttat tggaactggg caccgttaca ggcgccgtga gtggtttttt aattgccaaa
    18961 aaaatccaag cttctttttt atttttactt tttggcgttt ttcttttctt ttctgcggtc
    19021 atgatgctgc gtagccgtga tgagcacact tctacaaaaa atcatccatg gtcagaaaag
    19081 ctcaatctcg ccggttcttt tcctgatggc aaagggggag tcctccccta caatgtggac
    19141 aacgttccct ttggcctcac tgcgatgtat gcagctggag tcatttctgc actgctcgga
    19201 attggcagcg gcatttttaa agttctaaca atggatcgcg ccatgaagct gccgatgaaa
    19261 gcttcctcgg ccacttcaaa ctttatgatc ggcgtcactg ccacggccag cgccggggca
    19321 tttcttttgc gcggagacat ccgtcccgaa attgcggctc ccgtttcggt cggcattatc
    19381 cttggctctt ggatgggggc ccactctatg ataaaaattc ccactcaaat cctccgcaaa
    19441 attttcgtga tcgtgctttc tattgcctcc tttcaaatga ttctcaaggg gctttcatga
    19501 acactcaaga cttgcccgaa gatctattta ttaaagatcc gcttaaggat ccgcttctca
    19561 atctggaatt gagaatttct aaacttcttc attggggggt cagttgcgct gaagccttga
    19621 tgcttttggg ctggatcact ttttttgatt ttagcgaaaa tccgctttta aaatttcaag
    19681 attataaaga ggaaagcctg caaaagagct tgcaatccgc tctgcaagat caccactggg
    19741 gtctgttgct cgcctatgcc gggctcgttc ttttgatcag cttgcctatt ttacgggttc
    19801 ttatgaccgg aatttttttc gcaaagcaga gaaaccaaat tcttgccctg gcctccttct
    19861 ttgtttttgt aattttgctt tttagcttta cacttggtat ttaactttaa ccgagctttg
    19921 ccatcataga ttcactttga accattggga gaaagcgttg atttctcctg ggtgacactg
    19981 gccggcgatt ttaaaggggc cgactctaga gatagaacgc ggcgattttt tgtattggct
    20041 ttgaagctca ccggagttcc ttttttactg ctttcttcta gagccttata gatggcactg
    20101 tagcctcgac cactaggaat atagtaacta tcgttcagat ccttaaagaa gacctcagtc
    20161 atctcgagtt tacgaatgat ccgcaccgtg cctgagagta ttttctcatt ctcgccaagc
    20221 tgagggcgtc tttcttcatc cttggaaacc tcttgtactt cttgaatctc ttctcctact
    20281 tctttgaagt ctgaggcttg cggcaaggct cccgctttaa aacttatcaa taaaactcca
    20341 aagcccgccc caaacaatcc ccttattaac ctcataaaac ctcctgctga tccatagcag
    20401 aataaatcat tccctttctt tttaccaagt tctaggtact tttggtttag attctatgtt
    20461 aaagtacgga tcaatcatgc gtatcctcca cttgctttca caaatgcagc ccactggggc
    20521 ggaaacctat gccgtcaccc tagccaattg gctaacggat cagggccatg atgtgtttat
    20581 tatttcagat cgcatccatg taaagacacg tcagccttat attcccctgg ccgttcacga
    20641 taagcgcttg cccacacgga tcaaaagtac tttttttctt agaaaattct taagagaaca
    20701 taacatccaa attatccatt gccattcgcg cgccgctgct cgtctcgcct attgggcgac
    20761 gcaagggaca aaggtcgctg tggtgtcaac gatccatggc cgtcaaccta tttcactctc
    20821 taaaaaactt ctcaatatct acggcgagaa
//
LOCUS       DATBTE010000191         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2416188, whole genome shotgun sequence.
ACCESSION   DATBTE010000191 DATBTE010000000
VERSION     DATBTE010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2416188"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..1194)
                     /locus_tag="VIG33_07120"
     CDS             complement(295..1194)
                     /locus_tag="VIG33_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270644.1"
                     /translation="MKWKVFLVLSLFFSYGEIAVAETYGRTSAIYDYLPIARPSGLFA
                     CSNKIRAGLKNNRILNIAVVLGYSDLTDEHVDLVTDAFTLALLDRKLTSPCTYEHQGF
                     CEFKLIEGKEHQVNHYARNLVINESEDIAVNIFTINSSYSISNTDNKTKYKTLQGNQT
                     QTARNFYSWAQQNADMVFYEGHSRDGGGPDFSPPRPSYSGAVNYPWYRTNKPGLKFLL
                     SALNTAPEKPITLGLFSCASHVHFLKPLRQALPNAKLILSTKVVESGKTKLALLRTLE
                     SILNFECDSDLKARLRETSFVLN"
     gene            1434..2684
                     /locus_tag="VIG33_07125"
     CDS             1434..2684
                     /locus_tag="VIG33_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HEY8270645.1"
                     /translation="MELRAEDIQPLHQILISLMLGMLVGLQRQWADPSLGGIRTFPFI
                     ALLGTTCAVISEKIGTWMMGLGFFAILCLIILGNLIKRFPGELRTHSRLGTELAMLLV
                     FMAGAMVRIGPTWLAAALAGIVAVVLHAKIELHGMASRFNEKELKAIMQFTLISLVIF
                     PLVPNESLGPMKIFNPRDVWLMVVLIVAISLGGYIIYKFFGRKAGILLGGILGGIISS
                     TATTVTQAKMNRQDSDKSWTCALVIIVAWATVYIRLAFEVGLVAPSFGKIWFPLLTLF
                     VAAIVPVPFLWKKASQQRGMPLQSNPTELKTAFVLAALYSVILFAVAFAKKYAGTEGL
                     YITAFLSGITDMDAITLSTSRLVENGTLIPEEGWRTIVIAVISNTTFKGLIARFWGGK
                     VFFRILLVPWTIAMAAGIALLIFF"
BASE COUNT          750 a          514 c          623 g          830 t
ORIGIN      
        1 ggaaattttt ggctggttaa agtccatcgg tttaatgaga agaccaatgg taagaggctt
       61 gcgaaagttg gttggatgtt ttccttcggc cttgccgttt acaatttggt acgaatcaac
      121 aatctatgtg cacaataggc tcgaaaaatc actaaagcag ctcagagcag agtgaatggg
      181 aacatgggca cgatgagaaa aatctagttt ttttaaaaaa tctttcgaaa ttatgagaaa
      241 taaaaatcct ggaggtggga aaaacaggcc cgaaggccgt ttttcaccga cctgctaatt
      301 taatacgaaa gaagtctccc gcaatcgtgc ctttaagtca gagtcacatt cgaaattaag
      361 aatggattcg agtgttcgta aaagagcgag cttcgtttta ccagattcaa ccaccttagt
      421 gcttaagatc aattttgcat ttggtaaagc ttgtctcaga ggttttaaga aatgaacgtg
      481 ggaggcacag ctaaataatc ctaacgtgat aggtttttct ggtgcagtat taagtgcgga
      541 aagcaaaaat tttagaccag gtttgttagt acgataccat ggataattca ctgctcctga
      601 ataggagggc cgtggtggtg aaaaatcggg acctccgccg tcgcgcgaat gaccttcata
      661 gaagaccatg tcggcattct gttgggccca gctatagaag ttccgagcgg tttgggtttg
      721 gttcccttga agagttttat atttagtttt attatcagtg ttgctaatgc tataactact
      781 gttaatagta aagatattaa cagctatgtc ttcgctttca ttgattacta agttgcgagc
      841 ataatgatta acttgatgtt cttttccctc gatcagtttg aattcgcaaa agccttgatg
      901 ttcataggtg caggggctgg ttagctttct atcaagaaga gctaatgtga aagcatccgt
      961 tactaaatca acatgttcat cagtaagatc gctataacct aatactacgg caatgttaag
     1021 aattctgttg ttttttaatc cagccctgat tttgttagaa caggcaaaaa gccctgaggg
     1081 tcttgctatt ggaagataat cataaatggc agaggtgcga ccgtaggttt ctgcaacggc
     1141 tatttcccca taagaaaaaa ataaggataa gactaaaaat actttccatt tcatgttccc
     1201 tccaaggcat cgaaattatt actgacttac catgtagaga gaggtgaact caaaggagat
     1261 tagctttaac tcgcattaac tgtagctttt tcacaggttc cccgttctat tttagagggt
     1321 aagcttagtg ttgaaagtca gagtggaagc agtcttgagt aaatccgtcc tcaaaatctc
     1381 aggaagcatg aaaagtactt gctagaaaca gaaaaaaaca tctaataaac gatatggaac
     1441 taagagccga agatattcag ccactgcacc aaatccttat ttctctcatg ctaggaatgc
     1501 tggtggggct tcagcgccaa tgggccgatc cttccctggg cggcatcaga acatttcctt
     1561 tcattgctct cttggggaca acgtgtgcgg ttatctccga gaaaattgga acttggatga
     1621 tgggacttgg tttttttgcg atcctttgtc tgataatctt aggaaatcta attaagagat
     1681 ttcctgggga gttgaggact cattcaagac tgggcacaga gttagcgatg cttcttgtct
     1741 tcatggcggg cgctatggtg agaataggtc ctacttggct agcggcagcg ttagctggta
     1801 ttgttgctgt agttcttcac gcaaaaattg aactccatgg catggcttct cgattcaacg
     1861 aaaaagaact taaggccatt atgcagttta ccctcatttc actggtgatt tttcctttag
     1921 tgccaaatga aagtttggga cctatgaaga tctttaatcc acgtgatgtc tggttgatgg
     1981 tagttcttat cgtggcgatt agtcttggtg gatatatcat ttataaattt ttcggaagaa
     2041 aagccggaat tctattggga ggaatccttg gtggaatcat ttccagtact gcaaccactg
     2101 tgactcaagc caaaatgaat aggcaggatt ctgataagag ctggacttgc gctttggtaa
     2161 tcattgttgc ttgggcgacg gtttatatta ggttggcttt tgaagtggga cttgtagcgc
     2221 cttcgtttgg caagatttgg ttcccgcttt taactctatt tgtagcggca attgttccgg
     2281 tgcctttttt atggaaaaaa gcttcgcaac aaaggggcat gcctcttcaa tcaaatccca
     2341 cggagctcaa gaccgcattc gttctagctg ccctctattc tgttattctt ttcgctgttg
     2401 ctttcgcaaa aaaatatgcg ggaacagagg ggctgtatat aactgcattt ttatctggga
     2461 ttactgacat ggatgctatc actctttcca cttcgcgcct ggttgaaaac ggaaccctaa
     2521 ttcctgagga gggctggcgc actatcgtga ttgcagtcat ctcgaatacg acttttaagg
     2581 gactcatagc tcgattttgg ggtggaaaag ttttttttcg aatccttctc gtaccatgga
     2641 ctatcgcaat ggcagcggga atagcactgc taattttctt ttaaataaaa atacttccca
     2701 attcgattga attccgg
//
LOCUS       DATBTE010000192         3404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2211368, whole genome shotgun sequence.
ACCESSION   DATBTE010000192 DATBTE010000000
VERSION     DATBTE010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2211368"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VIG33_07130"
     CDS             <1..374
                     /locus_tag="VIG33_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270646.1"
                     /translation="SGLVCIALLCGGFFMSLIDLTHQYDEAGTVYLTASNASGLILVL
                     LSLGTFFWIFTSAWPGAQEMKAAKKKEESVTQAKTASLEHALSLLVMDFIKEREQKRE
                     RYAREHYNPTAEKESPSLHDN"
     gene            421..1197
                     /gene="lgt"
                     /locus_tag="VIG33_07135"
     CDS             421..1197
                     /gene="lgt"
                     /locus_tag="VIG33_07135"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEY8270647.1"
                     /translation="MISWNVSPTIVEMGPLQLRWYGLMFLIGFLIGYQGMRKICIWEK
                     KPLEKLDSLLTHIFLGTLIGARLGHCLFYEPGYYLSHPLDIFKIWEGGLASHGGALGV
                     LLAIWLFSRKHTEFRLMWIFDRVAIFVVLTGAFIRIGNLMNSEIIGRPTDVSWAFVFE
                     RIDQLPRHPTQIYESLCYFIITILGWITYRKYKAKLPSGRIFGLTIALIFTARFFIEF
                     FKENQEPFEAAMPVNMGQLLSIPFMLAGIYFYAQSRISPK"
     gene            1379..3001
                     /locus_tag="VIG33_07140"
     CDS             1379..3001
                     /locus_tag="VIG33_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270648.1"
                     /translation="MKYVYILENDEKFRDEMIEAIHKSDPLLVVHYFSSLDEFSKWIK
                     LVIQVGPQALPRGGTAAPGQLNTAEDTHQLSLIIAKNELLGVKHMALLKKTRELFIQK
                     GLCPAEDPTSLVLTTFENPDFDIKLAEDRIINNVIFKPFDKLILQQHLNFAIGGRHPP
                     SQYAVHNMKTAATIEMLKEVEIEAFSDVGFISISNRPITVGSVAKYYSSAFISLRHKS
                     MLALCLRSDPHPDRKDYYRCSFTYLGADSFQITNIRKQVRQKDAQTFQYEWISKFPIS
                     PVEIVLISSDMDTAQFFKENIEKNYHNAKVRLFAKLQDFMHAIDPEVAKKDKREFDSK
                     NRITAPINAIFGDQYLFEDNFKDRWEAILETVKMKTPPPRNNPSQKTELFILTKKNHS
                     DPEKKALSEIVKDIFLMPMDTLYIIKKLALFLPQLSPLEKIQLPTIQYAEIAKAATLI
                     EINEFSEAGLVMKYYRPITPGAFREFILWLPHELELPEFLATCNYHEESKNEKGVFYN
                     QFVFFGTSDHYLKHIRRWILQNHLLSKEAESA"
     gene            3022..>3404
                     /locus_tag="VIG33_07145"
     CDS             3022..>3404
                     /locus_tag="VIG33_07145"
                     /inference="COORDINATES: protein motif:HMM:NF022439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2796 domain-containing protein"
                     /protein_id="HEY8270649.1"
                     /translation="MRVYLIFAALLLSLSTPAAENHKHREHRAHLHGSAELSLGFDGI
                     QGKIEFKSPSDSIIGFEHMAKSSADKKIKDEALKKFETKISEIISFDRSLKCQITKDK
                     IEIVAESDTHSDTTATFTAKCEKSP"
BASE COUNT         1013 a          756 c          651 g          984 t
ORIGIN      
        1 tcagtggact tgtgtgcatc gcccttcttt gtggaggctt ctttatgagc ctgattgatc
       61 tcacccatca atacgacgaa gcaggcactg tttatcttac ggcaagtaac gccagtggat
      121 tgattttagt gcttctctcc ctgggtacct tcttttggat attcacttcg gcatggcctg
      181 gagcccaaga aatgaaggca gctaagaaaa aagaagaaag cgtcactcag gctaaaactg
      241 cttcactcga acacgccttg tccctattag tgatggactt tataaaagag agagaacaaa
      301 aacgcgaacg ttacgcacga gagcattaca atcccactgc agaaaaagag tctccttcat
      361 tgcacgataa ttgaaaataa agctttacgt ttctgccaag cacgagcgat aatcccataa
      421 atgatatctt ggaatgtaag tccgacaatt gtagaaatgg gtccattgca acttcgctgg
      481 tacggcctga tgtttttgat aggtttttta attggctatc aaggcatgcg aaaaatttgc
      541 atatgggaaa aaaagccgtt agaaaaactt gattcgttat taacgcatat ttttctcgga
      601 accctgattg gcgctcgcct tgggcattgc ctattttatg agcctgggta ttacctttct
      661 caccccttgg atatttttaa aatatgggaa gggggcttgg ccagtcatgg tggcgcccta
      721 ggtgtcttgt tggcgatttg gctcttttct cgcaagcata ctgagtttcg cttaatgtgg
      781 atctttgatc gtgtggccat ttttgtggtg ctcacgggcg cattcatccg aattggcaac
      841 ttgatgaatt cggaaatcat cggacgtccc acggatgttt cttgggcctt cgtatttgaa
      901 agaattgatc aattgccaag acatccaacc caaatttacg aaagtctttg ttactttata
      961 attaccatcc tcggctggat cacttatagg aaatacaaag ctaaattacc ttcgggacgt
     1021 attttcggat taaccattgc cctcattttt accgcccgat tcttcatcga attcttcaaa
     1081 gaaaatcaag aaccctttga agcggcgatg ccggtaaata tgggtcaact tctcagtatt
     1141 ccttttatgc ttgcgggtat ttacttttat gctcaatcta gaatcagccc taagtagggc
     1201 ccacaagaat ccgttgagtt tttccacagc atatgcgaat tcccattctg agacgaatat
     1261 tagaatttgc tccaggaatc taacattgaa acattcaatt taaactatcg tctagaaaca
     1321 agtattatgt ttcagtttga tattaccttc tcagcagatt ttaggctata ctgtatttat
     1381 gaagtacgtc tacattcttg aaaatgatga aaaatttcga gatgagatga tcgaggccat
     1441 tcacaaatcc gatcctctgc tcgtcgtaca ttatttctct tccctggatg aattttcaaa
     1501 atggatcaaa ttagtaatcc aagtgggccc ccaagccctt ccccgaggag gcacggcggc
     1561 tcccggacag ctcaacaccg ctgaggacac tcatcagctt tctctgatta ttgcaaaaaa
     1621 tgaactcttg ggagtcaagc atatggcctt gctcaaaaag acccgggagc tatttataca
     1681 aaaagggctt tgccctgcag aagatcccac ctccctagtg cttacaacct ttgaaaaccc
     1741 tgacttcgat attaaacttg cagaagatcg tattatcaat aacgtgatct ttaagccttt
     1801 tgataaattg attctgcagc agcatttaaa ctttgccatt ggcggacgtc atccaccctc
     1861 tcaatatgct gtccataaca tgaaaaccgc agcgacgatt gagatgctaa aagaagtgga
     1921 aatagaagcc ttttctgatg tggggtttat tagcattagc aatcgaccca tcacagtcgg
     1981 ctctgtggcc aaatactaca gttccgcttt tatctccctt cgtcataaaa gcatgttggc
     2041 gctttgtcta cgttctgatc ctcatccaga tcgcaaagat tattaccgtt gtagttttac
     2101 ttatctcgga gcagatagtt tccaaattac caatatccgg aaacaagtcc gccaaaaaga
     2161 cgctcaaact tttcagtatg aatggatttc gaaatttccg atctcgcctg tggaaatagt
     2221 tttgatctct tccgatatgg atacggccca gtttttcaag gaaaatatag aaaaaaacta
     2281 tcataatgcc aaagtccgtt tattcgctaa attacaagat tttatgcatg cgatagaccc
     2341 tgaggtggca aaaaaagata agagggaatt tgattctaaa aatcgaatta cagctccaat
     2401 aaatgccatc tttggcgatc agtatttgtt cgaggataac tttaaagacc gctgggaagc
     2461 tatcttagaa accgttaaaa tgaaaacccc gcccccccgt aacaatcctt ctcagaaaac
     2521 cgaactcttt attttaacta aaaaaaatca ctccgatcct gaaaaaaagg ctcttagtga
     2581 aatagtaaaa gatattttct taatgcccat ggacaccctt tatatcataa aaaaattagc
     2641 tcttttctta ccccaactca gccccttgga aaaaattcag cttcccacca tccaatatgc
     2701 tgagatcgcc aaggccgcaa ccctcattga aatcaacgaa ttctctgaag cgggactggt
     2761 tatgaaatac tatcgtccca tcactcctgg agctttccga gagtttattc tttggctgcc
     2821 ccatgaacta gaacttccag agtttctcgc aacctgcaat tatcacgaag aaagtaaaaa
     2881 tgaaaaaggg gttttctaca atcaatttgt attttttggg acgagtgacc actatctgaa
     2941 acacatccgt cgctggattt tacaaaacca cttgcttagc aaagaggctg aaagcgctta
     3001 actccagtca aaggatactt tatgcgagtt tatttaatat ttgccgccct tctgctttct
     3061 ctttcgaccc cggccgcaga aaaccataaa catcgtgaac accgcgcaca ccttcatggc
     3121 tcagcggagc tttctcttgg ctttgatggt attcaaggaa aaattgaatt caaaagtcct
     3181 agcgacagca ttattggctt tgaacatatg gccaaatcaa gcgcggataa aaaaattaaa
     3241 gacgaggctt taaaaaaatt tgaaacaaag atttctgaga tcatcagttt cgacagatct
     3301 ttgaaatgcc agataaccaa agacaaaatt gaaatagttg ctgaatctga tactcacagt
     3361 gacaccacag ccactttcac tgcaaagtgt gaaaaatctc cgat
//
LOCUS       DATBTE010000193         4347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1937104, whole genome shotgun sequence.
ACCESSION   DATBTE010000193 DATBTE010000000
VERSION     DATBTE010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937104"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VIG33_07150"
     CDS             complement(<1..403)
                     /locus_tag="VIG33_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY8270650.1"
                     /translation="MTGFVNFINDSGVVGWIILLTGIGSMVLVLERAKALYFSYGMNV
                     DEFMAKTQNLVLAKKLDEALLMCAQMEQKPMARAFKTILEKADRDDETIFQASDIAMS
                     ENIPLYTKRLHYLSMLANVATLLGLLGTIHGL"
     gene            complement(431..1006)
                     /locus_tag="VIG33_07155"
     CDS             complement(431..1006)
                     /locus_tag="VIG33_07155"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY8270651.1"
                     /translation="MNTNTATHHEWYILRGEMKYGPYEYSALIHMIQTGELFDYNYAW
                     APHMESWVTLSEIPEFSKSTLTQLIKTQNHLSGTFIKRGSPRIEIERDIYAHNNNNFF
                     DGKCLSISDNGALLLLNDPLLLPGQELVIHFRNLAENSSSGFNVKAEVIRKNFTKQRI
                     NFKSGLHYAVRFLQVPKQGQDQIQNMILKKV"
     gene            complement(1020..2096)
                     /locus_tag="VIG33_07160"
     CDS             complement(1020..2096)
                     /locus_tag="VIG33_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270652.1"
                     /translation="MKRRSATHKLTLILVSAVFLAIGCNKSADSFSLLADASSYKQNA
                     QYIPRKVDILWVIDNSGSMDNSQTRLANSFSAFISKFMTTDSDFHMAVTTSDAFLAPY
                     DAQYANFSRIRDGVGSVHSGVFVMDKNTPDMESVFLKNIKQGIKGSGDERAFSSFKAT
                     LSDPWNASFRRPDAFLAIIIVSDEDDFSHNDSTNGLKSYYFSENYNDAKMYSVQSYVD
                     DLTNYTANAGAGKNFSVNTISILDSVCLAQLKDSAQKIGQRYIALADATGGQKISLCS
                     DFSQSLQVLSESIVELSSVFQLTREPLPETISVVVDGTEVPQNATNGWTYDATTISIA
                     FHGTAIPTAGADVRINFDPKGVKN"
     gene            complement(2430..3290)
                     /locus_tag="VIG33_07165"
     CDS             complement(2430..3290)
                     /locus_tag="VIG33_07165"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEY8270653.1"
                     /translation="MTHQKVAKLINVATLLILILSYAELGMAITKGKSLDQATPAQRK
                     AFESVVFLSVTGGSQSTGSGTAIHIGNGVLLTAAHVIYGFNDEAMAIVALNSEKNSLG
                     GGLIEPEWERISAPKLGTLTDKYGEYSIPDLALLIPKDPDLRAKIAGLPVIKISTEAP
                     AIGETFTYLGNGPGASNGLEQFGQLKISEVGEIYSTSLSNKKNGVSALEPGDSGGSLF
                     RENAAGVLELVGIGFEAMNQGKESLSLINKLQDSRIKEWLSSAYEKINSTPLLWAQGL
                     NRSCKSVFIF"
     gene            3486..3875
                     /locus_tag="VIG33_07170"
     CDS             3486..3875
                     /locus_tag="VIG33_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270654.1"
                     /translation="MPTAAGDKMYRINTKARFSSKDFQRSGSGMGFYTISGEQDPGMN
                     GQSISLRFFPDGQGGEIVGLRIHLNIAGNGKRTKEISSVSAYESPGVLGSIKGEAYLS
                     TGDDGNLVQSIGLRAECESSSFFLLGS"
     gene            4144..>4347
                     /locus_tag="VIG33_07175"
     CDS             4144..>4347
                     /locus_tag="VIG33_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270655.1"
                     /translation="MAKKHLIVLVLSLKACFVFASENSTGADCINKINGLSDRLSSTT
                     YQINGISQYRPGDIHYQLVDLKAV"
BASE COUNT         1231 a          919 c          934 g         1263 t
ORIGIN      
        1 tcaatccgtg gatagtaccc aaaagaccta acaatgtagc cacgttcgcg agcattgaaa
       61 gatagtgcag acgttttgta tagagaggga tgttctcgga catggcaatg tcggaggcct
      121 ggaagatggt ttcatcgtct ctgtcggctt tttcaagaat tgttttgaaa gcccgagcca
      181 ttggtttctg ttccatttga gcgcacatca acaaggcttc atctaacttc ttagctaaaa
      241 ccagattttg cgtttttgcc atgaactcat cgacgttcat gccgtaagaa aagtaaagtg
      301 cttttgccct ttcaagcacc agaaccatag agccaatacc cgtaagaagg atgatccaac
      361 ctaccacacc tgaatcgttg ataaaattca caaagcccgt catataaatt cctccaattt
      421 tttttcaaaa tcatactttt ttcaaaatca tattttgtat ttggtcttgt ccctgttttg
      481 gcacttggag gaaacgaaca gcatagtgca gcccagactt gaagttaatc ctttgtttag
      541 taaaattctt tctaatcact tcggctttca cgttaaaacc tgaagaacta ttttcagcta
      601 aattacgaaa atgaatcacc aattcttgtc ctggcaaaag caaagggtca ttgagcagca
      661 aaagagcgcc attatcactg atagataaac actttccatc aaaaaagttg ttgttattat
      721 gggcgtaaat gtctctttct atctcaattc gaggcgaacc acgtttgatg aaagtgcctg
      781 acaaatggtt ctgagtctta ataagttgag tcagagtgct cttggaaaat tctggaatct
      841 cactcaaagt cacccaactc tccatatgcg gagcccatgc atagttgtag tcaaagagct
      901 caccagtttg aatcatatga atgagcgcac tgtattcgta gggaccgtat ttcatttccc
      961 cgcgtaaaat gtaccattcg tgatgagtcg ccgtatttgt attcatcctc aatcctctct
     1021 tagtttttta cgcctttagg gtcgaaattg attcttacat cagctcccgc tgtaggaatg
     1081 gctgtgccat ggaaagcgat ggaaattgta gtcgcatcgt aagtccatcc attggttgca
     1141 ttttgaggaa cttcagtgcc gtcaacgact acggagatgg tttcagggag aggttcccga
     1201 gtcagctgga agacactgga aagttccacg atggattcag agagaacttg caagctctgg
     1261 ctgaagtcac tgcagaggct gattttttgg ccgccggtag catcggccag ggcaatataa
     1321 cgctggccta ttttttgggc gctgtctttc agttgggcta agcaaacgct gtctaaaata
     1381 ctgattgtat ttaccgagaa gtttttaccc gcacctgcat tggcagtata gttagttaag
     1441 tcgtcgacgt agctttgtac ggagtacatt ttagcatcgt tgtagttttc tgagaagtag
     1501 tagctcttca aaccatttgt ggagtcatta tgggagaagt catcttcatc actgacaatg
     1561 atgatggcta agaaggcatc tggacgacgg aagctggcat tccacggatc actcagagtc
     1621 gctttaaaac tcgagaacgc gcgttcatcg ccggaacctt taattccttg tttaatattt
     1681 tttaaaaaca cactctccat gtccggagtg tttttatcca tgacaaagac accggagtgc
     1741 acgctgccga cgccatcgcg gatgcgggag aaattagcat attgggcgtc gtacggagct
     1801 aagaaagcgt ccgaagtcgt gacggccatg tgaaagtccg agtccgttgt cataaacttg
     1861 ctgatgaaag cggaaaagct attcgccaaa cgagtttgag aattgtccat tgatccggag
     1921 ttatcgatca cccacaggat atccactttg cgtggaatat actgggcatt ttgtttgtag
     1981 ctgctcgcat ctgctaacaa agaaaatgaa tccgcggatt tattacaacc gatcgccagg
     2041 aagaccgctg aaacaaggat gagcgtaagt ttgtgtgtag ctgagcggcg tttcattgag
     2101 aacctccagt tcaaaagacc tatcgactag atctgtggct agtttaatgg gtccacagac
     2161 taaaatcgaa ttgaccgcga agtgcttgaa acaactctta ttttgctctt gaaatctcaa
     2221 atttagattt gttaaatgtt cgaaaccaca agaggaatat aaaaattaac tatttttaaa
     2281 aaaaattctg gcgtcgaact gttagacgga atatttagga ttctcactcg acttcagaga
     2341 cgttcgtgag aatgtttaat gaactggaaa agtttctgac agtttaagaa ggttttcttt
     2401 tttctactct ataagcgaga ggccccttgt taaaaaataa aaacgctctt gcagcttcta
     2461 ttgaggcctt gcgcccaaag caaaggggtt gagtttattt tttcataggc actagacaac
     2521 cactctttta tcctggaatc ttgcagctta ttaattaaac tgagtgattc ttttccttgg
     2581 ttcatcgcct caaaccctat tcctactaat tcaaggaccc cggctgcgtt ttctcgaaaa
     2641 agggatcctc ccgagtcacc gggctccaat gccgatacgc catttttttt atttgaaagg
     2701 gaggtcgaat aaatttcccc aacttcactg attttcaatt gaccaaattg ttccaagcca
     2761 ttgctggctc ctgggccatt gccaaggtaa gtaaaagttt ccccaatagc tggcgcctcg
     2821 gttgaaattt taatcacagg aagccctgca attttagcgc gtagatctgg atctttgggg
     2881 ataagcaaag caaggtctgg tattgagtat tctccatatt tatcagtcaa agtccctagt
     2941 ttcggagcag agatccgttc ccattcgggt tcaatcagtc caccacccaa agagtttttt
     3001 tcagaattaa gggctacaat agccatcgct tcatcgttaa aaccataaat aacatgggct
     3061 gctgtcagta acactccatt tccgatgtga atagctgtcc cagacccagt tgattggcta
     3121 ccaccagtca ctgagagaaa aaccacactt tcaaaagctt ttcgttgagc tggcgttgct
     3181 tggtcgagcg acttgccttt agtaatcgcc attcccaatt ctgcgtaaga cagtattaaa
     3241 ataagcaaag ttgcgacatt aataagttta gctacttttt gatgagtcat ccatttctcc
     3301 tttagcagat aattctgctt catatttaac tttgcaaatt ggatgcccac agccaatcga
     3361 agaaaaataa aatgaacgca aaacaatgag gtttcagtcc attatttaga cgtagtgaag
     3421 ttgacagatg acataaatag tcagcggaat tggtcgcctc tccgtttttt agaatggggt
     3481 agtttgtgcc aactgctgcc ggtgacaaaa tgtatcgtat aaatacgaaa gcacggtttt
     3541 caagtaaaga ctttcagcgg tcaggttcgg gaatgggatt ctacactatc agtggcgagc
     3601 aagatccagg tatgaacggc cagtcaattt cactgagatt ttttcctgat ggacaaggcg
     3661 gcgaaatcgt tggcttgcga attcacctga atatcgcagg aaacggaaaa agaacgaaag
     3721 aaatctcatc cgtgagcgct tacgagagcc caggagtttt aggcagcatc aagggcgaag
     3781 cttacttgtc taccggagat gacgggaatc ttgtgcaatc gattggtttg agagccgagt
     3841 gtgaaagctc ttcctttttt ctattgggtt catgacagac ttaggctccg taggatttaa
     3901 actcattgct ggcgcattgg cataaactac ccctcataaa aaatggagat ataaactttt
     3961 attgatttat ggaaccagcc atttacgacc ctctgtaaag agtttagaca gtctcataac
     4021 ctcataatta ttttatccac tgctgagcgt ggaataatta ccgcatagtt acctaagcaa
     4081 aggagccgtg cccctgcaaa tcgggaaatt aatcccacag gccttgaagc ggaattaccc
     4141 attatggcga aaaaacatct tatagtttta gtattatcct taaaagcttg ctttgtattt
     4201 gccagtgaga attcaaccgg agctgactgt ataaataaaa tcaatggtct ttccgaccgt
     4261 ttgtcctcta caacttatca gatcaacgga atttctcagt atagacctgg ggacatccat
     4321 tatcagcttg tcgatttgaa ggcggtg
//
LOCUS       DATBTE010000194         1968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1117593, whole genome shotgun sequence.
ACCESSION   DATBTE010000194 DATBTE010000000
VERSION     DATBTE010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117593"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VIG33_07180"
     CDS             <1..1015
                     /locus_tag="VIG33_07180"
                     /inference="COORDINATES: protein motif:HMM:NF019259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1592 domain-containing protein"
                     /protein_id="HEY8270656.1"
                     /translation="PYQLAARLSFFLWQSVPDSSLFSAAADGSLTTSSTLKAQVLRML
                     KDPKGQRFATLMTNEWLGVNQILGLGLTTINTATLNAMVAETQFMFEDIIRSDVSFAN
                     SLAADYSFLNKTLADYYGVPFNGAVSTQFYKTSLASTPRRGVLNQAAFLIATSGSPNQ
                     THPVLRGKVVARKIGCYDIAPPPATLDTSLPPALPANSTPAEILALHTQRVQCAGCHN
                     IIDPHGLPLETYDSRGLWRTNYLEIAGRGIDVSGTLPSGERFSSTQEFMRTLATSSTI
                     KSCLVRKVMAVGLTRNVASTEDQCNAQQISELSMNANSKFSDLILNIIASRQFRMQTT
                     EAP"
     gene            1206..>1968
                     /locus_tag="VIG33_07185"
     CDS             1206..>1968
                     /locus_tag="VIG33_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270657.1"
                     /translation="MTQLTRKSISQIGLTLCVIFQSTLVLAEEYHQFSPSLIQRVVAP
                     DPHLSEPFDATKKQDLIQFPFIPGKLMEGLMKSLDQKKSIKRQMQHLYGLNDYTVSYI
                     TAQPDTWAKVGDHLSGVSPNCFLCHSSFINGQFVPGIGNTRVDLKNFLQDLVFKNAKK
                     DLKAVPPFLKKLKDVYKAQTGDKAKAFVDRSALLYSVFDFMLDPTQQSLAAGHTNPWS
                     FAEYLVNWRDDNMDYWQSNSLLSHKGGRGFKNAEVV"
BASE COUNT          513 a          533 c          429 g          493 t
ORIGIN      
        1 tccctatcaa ctcgccgcca ggctttcttt tttcttatgg caatccgtgc ctgacagctc
       61 gctcttcagc gccgccgccg atggctccct caccacctcc tccacgctca aagcccaagt
      121 cttacgcatg ttaaaagacc ccaagggcca gcgcttcgcc acgctgatga ccaatgagtg
      181 gctcggcgtc aatcaaatcc ttggcctcgg cctcaccacg attaatacgg ccaccttaaa
      241 cgccatggtt gctgaaactc agtttatgtt tgaggatatc atcagaagtg atgtgagctt
      301 tgccaattcc cttgcggcgg attattcttt cctgaataaa accctggctg attactatgg
      361 agtgcccttt aacggagccg tgagcacgca attttataaa accagcctgg cctccacccc
      421 gcgccgaggg gtgctcaatc aagccgcctt cctgatcgcc acctcaggct cgccgaatca
      481 aactcatccg gtgctcagag gcaaagtcgt ggctcgaaaa atagggtgct atgacatcgc
      541 tccgccgccg gccactttag acacttcttt accccccgct cttccggcca actccacgcc
      601 ggcggaaatc ctggctctgc atacgcaaag agtgcagtgt gcgggctgcc ataatatcat
      661 cgatccccat gggctcccgc tagaaaccta tgactcgcgc ggcctctggc gcaccaacta
      721 tctagagatc gccggacgcg gcattgatgt cagcggcacc ctgccttccg gcgaacgctt
      781 ttccagcact caggaattta tgaggaccct ggccacgagt tccactatca agagctgcct
      841 agtgagaaaa gtgatggccg tgggcctgac ccgcaacgtg gcctccactg aggatcagtg
      901 caatgcccag caaatcagtg aactcagcat gaatgccaat tccaagttct ctgatttaat
      961 cctcaatatc atcgcaagcc gtcagtttag aatgcagaca acggaggctc cgtgagcgat
     1021 tttaaaattg aaaataatat cctatcacag gaacgacgta aaatacgtag acacccattt
     1081 tttctttaga acaatttaaa tccacattct tcttggcaca atcatcgcat gtcgagttcc
     1141 cgagctattt acttcggcaa atttgagaaa tcaatctcac cgagtttatt acgggatctc
     1201 atgccatgac tcaacttact cgcaagagca tcagccaaat cggcctaact ttatgtgtaa
     1261 tctttcaaag cacccttgtt ctcgctgagg agtatcatca atttagtccc agtctcattc
     1321 aacgggtggt ggccccggat ccccatcttt cagaaccctt tgatgcgacg aaaaaacaag
     1381 atctgatcca atttccattt atcccgggaa agctcatgga aggcttgatg aaaagccttg
     1441 atcaaaaaaa atccattaaa agacagatgc agcatttgta tggtcttaat gattacacgg
     1501 tctcctatat cactgcccaa cccgacactt gggccaaggt gggagatcat ttgagtggag
     1561 tctcgccaaa ttgttttctt tgtcattcaa gttttattaa tggccaattc gtaccgggta
     1621 ttggcaacac ccgtgtcgac ttgaagaact ttctgcagga tttggtgttc aaaaatgcaa
     1681 aaaaggactt aaaggcggtg cctcccttcc tcaaaaaact aaaagatgtc tataaagctc
     1741 agactggaga taaagcaaag gcatttgtgg acagaagtgc gcttctctat tcggtgtttg
     1801 atttcatgtt ggatccgaca caacaaagct tagccgcagg ccacaccaat ccgtggagtt
     1861 ttgccgaata tcttgtgaat tggcgggatg acaacatgga ttactggcaa tcaaattctc
     1921 tcttgagcca taaaggcggc cgcggattta aaaatgcaga agtggtgt
//
LOCUS       DATBTE010000195         3230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_776775, whole genome shotgun sequence.
ACCESSION   DATBTE010000195 DATBTE010000000
VERSION     DATBTE010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776775"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VIG33_07190"
     CDS             complement(<1..1096)
                     /locus_tag="VIG33_07190"
                     /inference="COORDINATES: protein motif:HMM:NF022580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HEY8270658.1"
                     /translation="MSVTQSLCEELPYWEFESEPISHVILSDGSVSSGIEILPLDIEC
                     FDESRINQLTLGLRSFVNSLPEGITCQFMVKVETDFEQKIDSHLKLVTTNIDFLKTLD
                     DARAENIKSQIQEDSVFRPRLFFFIKTEGAQKPSRFSLSQTKKFSEGFEKGYEDRLQI
                     LSQAIEGASQSLTSLGFLGSSLNKEDLIELLYRYLNPKRAKETTPPKISPRSALLEGD
                     SPRSQIVFGDLILDQEDFILDSMRTRVLTLKTLPEMTFAGMMSGFLALPFKYELFFSF
                     KIPEQAKEMKSLEQKRRLAHSLASSTSNRVTDLESESRLSQTTELIREIIETGQRIFQ
                     AELMIVLREPVNSEGLRRLNLHTKQVLSHFK"
     gene            complement(1093..1416)
                     /locus_tag="VIG33_07195"
     CDS             complement(1093..1416)
                     /locus_tag="VIG33_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270659.1"
                     /translation="MSDETLLTSTVPRTLETKNKILGFELLDVLLLLLNLSIQNLIFG
                     GTALKTPMVFGTSLVIGGLLFFLKKGKPDQYLPHFFEHMISATHRPANSLDEKYRPFV
                     KKDSL"
     gene            complement(1416..1703)
                     /locus_tag="VIG33_07200"
     CDS             complement(1416..1703)
                     /locus_tag="VIG33_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HEY8270660.1"
                     /translation="MINKKVVFFILILGLLVQITPEMGLASVESSLMGIQTKLTRVIL
                     PTLSIIGIAWAAFSLMTGNEKAKTHMLYAIIGSVIGFGAQAIVDFISETVH"
     gene            complement(1726..2118)
                     /locus_tag="VIG33_07205"
     CDS             complement(1726..2118)
                     /locus_tag="VIG33_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270661.1"
                     /translation="MFNNVIPIRKNDRFGVFLGSVTSSGEVVESTRVGTAYLKPGSKT
                     FRLKLWVFPNNQYFVAPHDQDPTKYVVLSLDEFQTPQGEVRSFWNPIGEGRLVGSFIK
                     LSIYLFSEDVFLCLFPENYEAKEDVIAS"
     gene            complement(2136..2390)
                     /locus_tag="VIG33_07210"
     CDS             complement(2136..2390)
                     /locus_tag="VIG33_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270662.1"
                     /translation="MSRTYKDKNVIYLPERKSPVKHKREHQLRGRYSKYKKEGPPLDA
                     DNCYDCGGPTDFQGGFLVCSECGHAEGVLDVIDYMELIAA"
     gene            complement(2390..2596)
                     /locus_tag="VIG33_07215"
     CDS             complement(2390..2596)
                     /locus_tag="VIG33_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270663.1"
                     /translation="MNAKKLNEPNENNRNPSIALVQNDEPKNKSVWKKIKNVFQKSEF
                     SYRDWQRLEEKRVRPQDHKQWRNF"
     gene            complement(2622..2891)
                     /locus_tag="VIG33_07220"
     CDS             complement(2622..2891)
                     /locus_tag="VIG33_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270664.1"
                     /translation="MNITKEDKKEKCPKCGSKEILTLGLDQMCCDCDWDNTLLLVNLG
                     QMDQPRLAAYEQFVKPKSFESLKSKTSTEATEVISAIRTKSPLSA"
BASE COUNT          923 a          684 c          665 g          958 t
ORIGIN      
        1 ttttaaaatg agaaagaacc tgcttcgtat gaaggttcag ccgacgtaag ccctcggaat
       61 ttacaggttc acgaagcaca atcatcagct ccgcctgaaa aatgcgttgt ccagtttcaa
      121 tgatttcacg aataagctct gttgtttgac taaggcgcga ttcactttca agatccgtta
      181 ctcggtttga agttgaactt gccagtgagt gagcaaggcg gcgtttttgc tcaagcgatt
      241 tcatttcctt ggcctgttct ggaatcttga acgagaaaaa gagttcatat ttaaatggca
      301 gggccaaaaa tccactcatc attcctgcaa acgtcatttc gggcagcgtt ttgagcgtta
      361 aaactctggt cctcattgaa tccagaatga aatcctcttg atccaggatg aggtccccaa
      421 aaacaatctg agatcttggg gagtccccct ccaaaagagc acttcgggga gaaatctttg
      481 gaggggtggt ttctttagct ctttttggat ttaaatagcg atagaggagc tcaataagat
      541 cctccttatt aagagatgat cctaaaaatc ccagagaggt cagggactgg cttgctccct
      601 caatagcttg agataatatt tgaagccggt cctcatagcc tttttcaaat ccttctgaga
      661 acttttttgt ctgacttaaa gaaaaccgac ttggtttttg agccccctca gtcttaataa
      721 aaaagaagag tctcggacga aagacagaat cctcttgaat ctgggattta atattttcag
      781 ctcttgcgtc gtccaaggtt tttagaaaat caatattggt agtaacaagc tttaaatggg
      841 aatcaatttt ttgttcaaag tcagtctcca cctttaccat gaactgacaa gtaattccct
      901 ctggtaagga attcacaaag gatctgagtc ctaaggtgag ttggttaatt ctagactcgt
      961 caaagcattc aatatcaaga ggaagaatct cgatcccgga ggagacagaa ccatcggata
     1021 aaataacatg ggaaatgggt tctgattcaa actcccaata gggaagttct tcacacaaag
     1081 attgagttac gctcataggg aatccttttt aacgaaagga cgatattttt catcaagact
     1141 atttgccgga cgatgggtcg cagagatcat gtgttcaaaa aaatgcggca gatattgatc
     1201 aggttttcct ttttttagga aaaaaagaag tcccccgatg acaagacttg ttccaaagac
     1261 catgggagtt tttaaggctg tgccgccaaa aataagattc tggatggaga gatttaaaag
     1321 tagtaaaaga acatccaaaa gttcaaaacc taagatctta ttcttggttt caagagtgcg
     1381 gggaactgtg gatgttaaaa gtgtttcgtc gctcatcagt gcaccgtctc tgaaataaaa
     1441 tcgacgatgg cctgagctcc aaaaccaatg actgaaccta taatggcgta gagcatatga
     1501 gttttcgctt tttcattgcc cgtcataagg gaaaaagcgg cccaggcaat tccaataata
     1561 gacaaagttg gaagaatcac ccgcgtaagt ttcgtttgaa ttcccataag ggatgactca
     1621 acgctggcaa gccccatctc gggcgtgatt tggactagaa gtccaagaat taaaatgaaa
     1681 aagacaactt ttttattgat catagagtca cctcctttgt ttaagttaag atgcaatcac
     1741 gtcctccttg gcctcatagt tttctggaaa taggcagagg aaaacatcct cgctaaatag
     1801 ataaatcgaa agctttatga aacttccaac aagccttcct tccccaatgg gattccaaaa
     1861 ggaacgaact tctccctgcg gagtctgaaa ttcatccaac gaaagcacga cgtacttcgt
     1921 agggtcctga tcgtgaggag ctacgaagta ttggttattg ggaaaaaccc aaagctttag
     1981 tcgaaacgtt tttgagcccg gttttaaata ggcagtgccc actctggttg actcaacgac
     2041 ctcaccagat gaagtcactg agccaagaaa aaccccaaac cgatcatttt tacgaatcgg
     2101 aattacatta ttaaacatat cgccacctcc ttttttcatg cagcaatgag ctccatataa
     2161 tcaatcacat caagtacacc ctctgcgtgc ccacattctg agcaaaccaa gaaaccacct
     2221 tggaaatccg tgggtcctcc gcagtcgtag caattatctg cgtctagcgg cggaccctct
     2281 tttttatact ttgaatatct tcctctaagc tgatgctcgc gtttatgttt cacaggactt
     2341 ttcctttccg gtaggtaaat gacattttta tctttataag ttctcgacat tagaagttcc
     2401 tccattgttt gtggtcttga ggtctcactc ttttctcctc aaggcgctgc caatctcgat
     2461 aggaaaattc agatttttga aaaacatttt ttattttttt ccagacgctt ttattttttg
     2521 gctcatcgtt ttgaacaagc gctatcgatg gattcctatt attctcattt ggttcattca
     2581 gtttttttgc attcatagtg ttacctcctt agttaaatat tttaggcact cagaggagac
     2641 ttcgtgcgaa tagctgaaat cacctctgtg gcttctgttg atgtctttga tttaagcgac
     2701 tcaaaagatt tcggctttac gaactgctcg taagcagcca gtcgtggctg atccatttgc
     2761 cctagattga ccagcaaaag agtattatcc caatcacagt cacagcacat ttgatcaagc
     2821 ccgagggtca aaatttcttt tgatccgcac tttgggcact tttctttttt gtcctctttt
     2881 gtaatattca tttcgtactc cctccttatt ggtttaaaat tccgtatttc ttacggtcct
     2941 ataaagcagg aatgggtcca cctcacctgg cagcagtaaa aggaggtgca actattgaaa
     3001 ttgatttgga tctttgattt ttttgccaaa aaacgctgag ttctacagat ggcgggtttc
     3061 cgccatagtg aatgctttca atcgatgtga atttaagggg ttagcggtaa aaatccggct
     3121 ggcggatatc caccaccggc tggcggacgt tcgccggata aaattgcagg tcgacctaga
     3181 cgacatcata gcaatttgat atgaaaggtt tttttacagg agacaacgct
//
LOCUS       DATBTE010000196         4175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_435889, whole genome shotgun sequence.
ACCESSION   DATBTE010000196 DATBTE010000000
VERSION     DATBTE010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435889"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="VIG33_07225"
     CDS             complement(<1..1423)
                     /locus_tag="VIG33_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270665.1"
                     /translation="MFSKRRSSQLIGGFVLASFVWLGGCSEFLDEKHQEPQTLEMANT
                     KFACLQSLPVQMSEFLKGHVSDGDIDQGFNCAQEALLYFKNKTKGTYPDAYSLDDLRN
                     FFGKYFLKKNNVSPEFGTQLFKLKRVLLGGTDYSITKLEIQRLVDLLDSVKKQAQLVA
                     PHVTVLLGQRDQATWSDVDAGITQLQESLWTLFKEVDLVRSNYSFSDLKKFMKGLSDF
                     INTTEPLLLTEQLNRDVELIEAVKNILIGEEPHLDNLNDWHSAVATVANFYKEALRYR
                     YFLKGNDFSTPASVQALLLSMNDALTLLENCFSMQHSGVVSFSSIDHFLVILEARKLL
                     PLGLSSASLKEAYKKGVLRALDPLRRGDARGLEGLERIHVLSLKHELKLYQLHQNMID
                     NLPFDPAARIGFAELKKAVEAFDPMAFILKSLTTESLELEVLLRAWNEGISLLLKPYP
                     VYYNEEGRQVVKTDVTTYRQTWKS"
     gene            complement(1413..2768)
                     /locus_tag="VIG33_07230"
     CDS             complement(1413..2768)
                     /locus_tag="VIG33_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270666.1"
                     /translation="MRKRNILKLAALVIGVGLGVLGRSAKAQLRSDLQLFSDSFISPS
                     FEANQKTNYQFVGLSLKSAPNAEDVLKMDIEGAVAIGAPLLNYLNISEIYFQNKQSEN
                     ETLIIGRKLIHWNDLDRRWNFGLWQPLFQWNPLNSQQQGLSGIFWQADREKYSVTIFA
                     SPVFVPNQGPTFEISEGTFVRGNPWFRRPPDSVRIFQEVTQVDYNFERPDENQAIFQT
                     SYGARLSFGTPETAMAQLSYMYKPSSELAIGYSGLLDTSKLRGVVDLKPQIFYHSLSG
                     LDLSQRWGVFRYGVSGIYDRPQKDLEFDEKWTHPEFKDAFLVSPFVEMILSDYTFSLQ
                     ALNVYGGEVHEVGDMAIPDRVPLTVRYPFQQAFKASLETRHALKGNTRLISRTSYTVS
                     QKNDFEYVQWNMNYRFSSLWSGFTELELVRAGERTLQNQNEIAQYKNLDRFLVGIAYV
                     F"
     gene            complement(2900..3904)
                     /locus_tag="VIG33_07235"
     CDS             complement(2900..3904)
                     /locus_tag="VIG33_07235"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEY8270667.1"
                     /translation="MVLSILEHWHKCRIVIQPRNRPENWKINSTGIGLVTLMLKPIFL
                     KNSLLIVCSVLSWANSGFAEEIYDARTLKSTSSQRLSMDQMVSKIPKGSIVLIAEKHN
                     VTSMQQGELDLLKALRNQGHKVNLGMELLQFSDQSNVDAYRKGTMTEEAFKASSWGES
                     DFNFYRDQILFPKDEDGEKTFAVNSPKALPIAVKTKGIAHLSAQEKAMLPPHFRLGRA
                     SYRERFREKMSYHVAGKKAMKRYFETQSVWDDTMAWKVCEAKSKSEGTLVLIVGQFHV
                     EYGDGLIHRIRARCGKTQPIVSVYQYLFYNDETYDVGQFAPSPKYGPIADYLVIVKED
                     "
BASE COUNT         1213 a         1016 c          834 g         1112 t
ORIGIN      
        1 aagacttcca agtctggcgg taagtggtga catccgtctt cacgacttga cgaccctctt
       61 cattataata caccgggtat ggtttcaata agaggcttat accttcattc caagctctca
      121 agagaacctc tagctccaga ctttctgtcg tcaaagactt gagtataaat gccattggat
      181 caaaggcttc caccgccttt tttagctcag caaaaccaat ccttgccgca ggatcaaaag
      241 gcaaattatc aatcatattt tgatgaagct gataaagctt caactcgtgc ttcaaagaca
      301 atacgtggat tctctcaagc ccttccaagc ctctggcatc ccctcggcgc agaggatcca
      361 acgcgcgaag cacccctttc ttgtaagctt ccttcaagga ggctgaactc aaccctaaag
      421 gtaagagttt tcgggcttcg agaataacca aaaaatgatc aatgctagaa aaagaaacga
      481 caccactatg ttgcattgaa aaacagttct ccaacaaggt gagagcatca ttcatggaca
      541 atagcaaagc ctgcactgaa gctggcgtgg agaaatcatt tccttttaag aaataacgat
      601 aacggagagc ttctttataa aaattcgcaa ccgtcgccac cgcactatgc caatcattaa
      661 ggttgtctaa atgcggctct tcacctatta gaatattttt caccgcttcg atcagttcca
      721 catccctatt taattgctcg gtcaacagca acggttcggt ggtattgatg aaatcactga
      781 ggcccttcat gaacttttta agatcactaa aactataatt cgatcttacc agatccactt
      841 ctttaaaaag agtccacaaa gattcctgca attgagtgat gccagcatcg acatcactcc
      901 aggtggcctg gtctctttgc cccagcaaaa cagtcacgtg aggcgccacc agttgcgctt
      961 gctttttaac agaatcgaga agatcgacca atctttggat ctcaagcttc gtgatcgagt
     1021 agtcggtacc accgaggagc actcttttaa gcttgaataa ttgggttccg aattcgggtg
     1081 acacattatt ttttttcaaa aagtacttcc cgaaaaagtt tctaagatca tccagggaat
     1141 atgcatctgg ataggtgccc ttggttttat ttttaaaata taaaagagct tcttgggcac
     1201 aattgaaacc ttgatcgata tcgccatcac ttacatgacc ttttaaaaac tcactcattt
     1261 gcacgggcaa agattgaagg caggcaaatt ttgtattggc catctccaag gtctgtggct
     1321 cttggtgctt ctcatctaaa aattcggaac agccccccag ccatacaaaa ctggctagaa
     1381 caaaccctcc tatgagttgc gaagaacgcc ttttagaaaa cataagcgat tcccaccaaa
     1441 aagcggtcta aattcttata ttgagctatc tcattctgat tttgcaaagt tcgttctccc
     1501 gcccgaacca actccagctc ggtaaagcct gaccacagag aagaaaaacg ataattcata
     1561 ttccactgta cgtattcaaa gtcatttttt tggctcacag tatagctggt gcgtgagatc
     1621 aagcgagtgt tgccctttaa ggcgtggcga gtttcaaggc tggccttaaa ggcctgctga
     1681 aacggatacc ttacggtgag cggaacccgg tccggaatgg ccatgtcccc aacctcatgc
     1741 acttctccgc cataaacatt caatgcttgc agggaaaaag tataatccga aagtatcatc
     1801 tccacaaagg gacttaccag aaacgcgtct ttaaactccg ggtgagtcca tttttcatca
     1861 aactcgagat ctttttgcgg gcgatcgtaa attccactca ctccatagcg aaacacaccc
     1921 cagcgttgcg agaggtccaa accactcagc gaatgataaa aaatttgtgg ttttagatcc
     1981 acgacgccgc gaagtttact cgtatccaag agtccactat agcctatcgc cagctcactg
     2041 gagggcttgt acatataact cagctgcgcc atcgccgtct ccggagttcc aaagctcaga
     2101 cgggccccat agctggtttg aaagatggct tgattttcat cggggcgctc aaaattataa
     2161 tccacttgag tcacttcttg aaaaatccgc accgaatcgg gaggacgccg aaaccacgga
     2221 tttccacgca cgaaagtgcc ctctgaaatt tcaaaggtag gaccttgatt gggaacgaaa
     2281 acaggtgatg caaaaatagt aaccgaatac ttctcacgat ccgcttgcca aaaaatccct
     2341 gaaagacctt gttgctgaga gtttaaagga ttccactgaa aaagcggctg ccaaaggccg
     2401 aaattccaac gtcgatcaag atcattccaa tggatcaact tacggcctat gatcaacgtt
     2461 tcattttcag actgcttgtt ctgaaaatag atctcagaga tattaaggta attcagcaat
     2521 ggagctccaa tggccacagc gccctcaata tccattttta gcacatcttc cgcattgggt
     2581 gcgctcttaa ggcttaggcc cacgaattga taattggttt tctgattagc ctcaaaggaa
     2641 gggctgataa aggagtccga gaaaagctgc aaatctgaac gtagctgggc cttggctgag
     2701 cgccccaaga ctccaagccc taccccgatg acgagggccg ccagtttgag tatattccgt
     2761 ttccgcatga actctcctac ccggggattt attcatcaaa tactgtgcct gctaagaact
     2821 cttcaaaagg cgccgccatc ccggcacaga tgtcaaatta tggacagatg ccattttacg
     2881 ccgccgatcg tacagttttt taatcttctt taacaatcac cagataatcc gcgattggac
     2941 catatttagg agaaggagca aactgcccca catcataggt ttcatcatta tagaaaagat
     3001 actgataaac tgaaacgata ggttgagttt ttccacaacg ggcgcgaata cgatggatta
     3061 agccatcccc atactcaacg tgaaactgtc caacgatcaa aacaagagtt ccttccgatt
     3121 tactcttggc ctcgcaaact ttccaggcca tggtatcatc ccaaacactt tgtgtttcaa
     3181 agtaccgctt catggctttt ttacctgcga catggtaact cattttttcc ctgaaacgct
     3241 ctctgtaact tgcacgaccc aacctaaaat gaggagggag catcgccttt tcctgcgcag
     3301 acaggtgagc aatgcctttg gttttcactg cgattggcaa agccttgggg ctattgacgg
     3361 caaacgtttt ttccccatct tcatctttag gaaaaagaat ctgatcgcga taaaaattga
     3421 aatcagactc tccccaactc gaagctttaa aagcctcttc tgtcatagtc cctttacgat
     3481 aagcatccac attggactga tcagagaact gcaaaagctc cattcccagg ttgaccttat
     3541 gaccctgatt acgcaaggct ttcaacaggt ccaactcacc ctgctgcatg gaagtcacat
     3601 tgtgtttttc agcgatcaag acaatcgaac cttttggtat tttactcacc atctggtcca
     3661 tagaaagtct ttgggaagat gtggatttca acgtccgagc atcatagatt tcttcagcga
     3721 atccgctatt agcccaagat aacaccgagc acacgatcaa caaagaattt tttaaaaaaa
     3781 taggcttcaa catcaaagtt accaatccaa ttcctgtgga attaattttc caattttcag
     3841 ggcgattcct tggctgaata acaatgcgac atttgtgcca atgctctagg atggataaaa
     3901 ccattatgaa aacaaaagag gtgtataggc actttgcaag gtgccgaggc tgtcacggtg
     3961 gcgacagcgc ctctccgttt tttagaatgg ggtagtttgt gccaactgat gatagaaaat
     4021 atgccagacc taggctattg atttttatta aaacaagtct ttattctatc cgaatgtttt
     4081 cattggactt ctggttgtac tcaaacagaa gtaaagtcgc gctacgagtt tcaaaatgaa
     4141 taataagctc cttcgcgact tatgatagcc gtatc
//
LOCUS       DATBTE010000197        14287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1187377, whole genome shotgun sequence.
ACCESSION   DATBTE010000197 DATBTE010000000
VERSION     DATBTE010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 14287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14287
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187377"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..580)
                     /locus_tag="VIG33_07240"
     CDS             complement(152..580)
                     /locus_tag="VIG33_07240"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY8270668.1"
                     /translation="MIPKIHREKNPLHADDQSVGVQYDKSLVSREWGSKEGNETGYNR
                     TYGVYGSVARGWVDRPHQGKGPKNYQRSDESLQEEIVKVLTLDSEIDASAVEVEVKEG
                     EVFLTGTVPERKMRYWVEEAIYKTRGVRDVNNQIKVEKQP"
     gene            complement(604..1176)
                     /locus_tag="VIG33_07245"
     CDS             complement(604..1176)
                     /locus_tag="VIG33_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8270669.1"
                     /translation="MKVDNKDKKNKKKKPETRPMTGENENIGEEDIVKLILQDHKPLR
                     KLLKTLKNVENSIEDRQAAFEEFALLFISHIKPEEQTLYVYLKHDDKTRENGMEGDIE
                     HALADHLLKEAHRTADDALWSARVKIMSELIESHLSEEEEHVLPNFQKMTDVEDRREL
                     GRDFLKLKNRILEQGQRDVSEEPFESYINH"
     gene            1328..2128
                     /locus_tag="VIG33_07250"
     CDS             1328..2128
                     /locus_tag="VIG33_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HEY8270670.1"
                     /translation="MEKLSRREQEFIQTAAQFLEHPGFMIQLANAFGKPLDLLQKTLP
                     QNVQEKIASLVEKSLKKTLEISISTLPKAEDPSLPRQPLLQARHHTFAAAALGAIGGF
                     FGPGTIAIELPITTGIMFRSIANTAQSFGEDLQDSHVRLECLQIFAMGSPQSKADDAM
                     KSAYFSQRLAFYSFITKASEKGAATLLARFITRVASEYQIVVAEKILAETLPILGAAG
                     GAVINSSFTHYFNQTAYYHFGLRYLERKYGLESVQQVYFEAFKNFRSK"
     gene            2212..2994
                     /gene="proB"
                     /locus_tag="VIG33_07255"
     CDS             2212..2994
                     /gene="proB"
                     /locus_tag="VIG33_07255"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY8270671.1"
                     /translation="MAKNRRWVIKAGSKMVCDGGPLLMRAWMQQVALLRKKHGIEVIW
                     VTSGAIAWAVARTNFKKTKRTLPQKQALSAIGQPLVMDQYNLALQSANLLGSQVLLTA
                     GDMKDSERRKNLQNTLSELLRWKVIPILNENDAVATEEIRFGDNDSLASKVAIMMKAE
                     RLILMTDVEGLFDADPNKNSKAKLIPYRRRIGKADFALADRKTVSKVGTGGMYSKLLA
                     ADNANRTGITTHLVRGDSANNLLQIATGISIGTQFGGRHELE"
     gene            2981..4258
                     /locus_tag="VIG33_07260"
     CDS             2981..4258
                     /locus_tag="VIG33_07260"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY8270672.1"
                     /translation="MNSNNQLSYQINEEMLKSLRGAARTQRNLSAIEKNKALLKLAED
                     LTRSASDIFAANQKDLQCLAADTTRAFRDRLTLNPERIEGMMESLRQVAALPDPVGEL
                     IEDQTLKNGLQLKKIRAPLGVIFMIFESRPNVILEAFSLAFKSGNVILLRGGSESRFT
                     SEALYRVMRESLHSSGFVKTPFYGLEDYDRALVEALLKRKDFIDIVVPRGGEKLIDFV
                     QKTALMPIIKNDRGLCHTFVDEDADLEMAVKIVVNAKVQRPGVCNSLETVLVHEKIAG
                     DFLPKLYRATEDKKLEWHVDKISQEILLGKPQIIPAQPADWDTEYLDLIINCRVVKNL
                     EEALTHIEQHGSKHSEAIVTKSERKARHFQQEVDAAAIYWNASTRFTDGFEFGLGGEL
                     GISTQKLHVRGPVGLRELTSPRWIIEGTGQIRT"
     gene            4365..4673
                     /locus_tag="VIG33_07265"
     CDS             4365..4673
                     /locus_tag="VIG33_07265"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HEY8270673.1"
                     /translation="MNHEFRNFCEFYNFYLSQHSNRECRQLHFFGTSLFFIAVGYALV
                     TQSWLLIPLAGAIGYLLAWSGHFFFEKNRPATFHHPYWSARAGRLMYFQLLTGKLSLK
                     "
     gene            4902..6353
                     /locus_tag="VIG33_07270"
     CDS             4902..6353
                     /locus_tag="VIG33_07270"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEY8270674.1"
                     /translation="MASDVRNWRSIALLAVTMACLAPKAQAATLLFKTDFGADTKLYP
                     PVIAYSTAGWQDIAGTNLTTGRLGPINLWGATASGFDMIATSPVSLPTLGNYSTNEIQ
                     QVTGPNGTPVNALFQNLKVTGGVAQDAFMIRRASVDRGDKTDTGDVYYSYWFKFQSDL
                     HSQLGTQGANNNNWRVMSEWKTGGLNNTYGGDYRIITVVVQDPNGNLSWSTQGDSNAN
                     GIPMQVFWKIPNTTVPVPVGKWFKYEVFWHRSSGSDGRYWAAVNGQVIADRKGPNMGA
                     YNLPINRIMLANNYTGGRAPCQQWLTGLEIWNGFPCGVGVSCYGAPTPTPTPTPTPTP
                     TPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPAPTPVDNV
                     APLTAVTSPINGSKVSAKSNITIQATASDNVGVKTVTFYINGNLKCSVSTAPFQCNWR
                     VPRPRGRTYNLRTRATDAAGNVGVSRRVTVTSK"
     gene            complement(6448..7314)
                     /locus_tag="VIG33_07275"
     CDS             complement(6448..7314)
                     /locus_tag="VIG33_07275"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEY8270675.1"
                     /translation="MKKRILIPLFFFNLFFYSISGFAVVQGVELIENENLPSPFVSLN
                     FGLKGLAPDPCSATYLGNGKILTAAHCFLLNEYAKIDPEICIQDYTNLQKLCVHKADY
                     EVYFPPLEEQGYKAPTRKTHSVIRLPKPDLAVIVLKEGLRSKLPNLEVVHLISPSALS
                     LELNQKKFKLVGQGCADYIIPPGEIPKGVGVFRMADVLLNPLDSTLELNSIWTDRQGN
                     GGVCWGDSGGALMLVAEESPLKLTQIAVVSAMKTKYDPATGKPHLVTSIYTRLDRQEI
                     QNWLQNTILVLP"
     gene            complement(7473..8582)
                     /locus_tag="VIG33_07280"
     CDS             complement(7473..8582)
                     /locus_tag="VIG33_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270676.1"
                     /translation="MRSIKIFVGFVIWTLSSSAYAYPEMIRMGYVNCTACHVAPNGGG
                     LLTDYGRSISSEVLSTWAVPKEEQPGHGFLPEPPAWLKFGGDIRLIQTYVDNAQATAT
                     SSFPMQTDLDLGLNFEKIWYVQSFGLQGGPSQAPDQGKFVSHHFYALFNWNDEVYSRG
                     GKYLLPYGLNIPDHTSFIKRNLGFDEGQESYNIDIGSNSEKWNTFFALSFGRKDNNSV
                     DLEKGGSLQVGYNLGDTHKISGSFAYLKNGNSQRYLLGPQILWGFNSRWVFLGEYDYQ
                     EKSLEGATKVVEKGVVTYSRLQYEWTKGFHSYLLHQFSYLDFTQLRTRVNSSGVGVLF
                     YPRPHFEFSAEVDYSETAEINAYNTVAWALAHYYF"
     gene            complement(8570..9148)
                     /locus_tag="VIG33_07285"
     CDS             complement(8570..9148)
                     /locus_tag="VIG33_07285"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY8270677.1"
                     /translation="MKTALGAIFTMMGLGVNAGAAPLPVIKGHIEFLAIGKPSAIKIR
                     GKGDDLKSEAQWKEKQLSGKFTFNLESLDTGIELRTNHMKEKYLETGKFSGKFKNAEL
                     ELKPVALAQDPCKESVKVTQAPFEGTLNLHGVQHPVKGDFDATAEKGKGHAQIRFELN
                     ITDYKIEIPVYLGIKVTDQVQNTVDLDWVCAQ"
     gene            complement(9164..9865)
                     /locus_tag="VIG33_07290"
     CDS             complement(9164..9865)
                     /locus_tag="VIG33_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8270678.1"
                     /translation="MNLKSFFFISAFSLLACNSYKSKVESQPEDTVAPSSIDYAFVND
                     KVFQPKCFQCHSLAGGNKGGVNLESYDNVLVNLADIQIDIFTEDSMPPLSRGGPLGAY
                     EKNVLKMWLDAGAPHDEKPALEPEPTPTPPPVNPVVIGANWNDIYKNVFEPKCIVCHQ
                     AGEKAEDFPLTDKDWVVNPANGLVKAGSPPDSSLYVAITRTDRKVMPPLKTGMTLSAE
                     EIDAIKSWILNGAKD"
     gene            10073..11740
                     /locus_tag="VIG33_07295"
     CDS             10073..11740
                     /locus_tag="VIG33_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270679.1"
                     /translation="MKFLLMGLFLLVSLKLHAAIPDWSENPPAPAGANPYQLPSDLFP
                     QYRDAGKIHAQIYPVSITGMLPPYEPIRHLIEDSSANPLRNFLNSLVRGVSGLHSMQD
                     VFTSLGLHDYPQAHDQGVYAVPFPQGVRPDYKMGFGFIETSQGKGFSFSCAACHSAHL
                     FGKTVLGMTNRFPRANEFFIEASKATHFVDPDLFKVYANATEGETQMVKQLKENMKYV
                     GFKKPLTLGLDTSLAHVSLSLNHRNKDPYATYSGVFASNPRPDPILDSSPADSKPAVW
                     WNLKYKNRWLSDGSVVSGNPILTNLLWNEIGRGADLVKLEPWLQQNEKIVQEITTAVF
                     SSEAPLFTDFFPAEKIDIAKAKEGEVIFNQTCSRCHGHYDKAWNLPHESELSPIEKLK
                     TTAVRYPTQTQVKDVGTDPYRYRGMRSLEKLNDLAISLKNGILIRAQQGYVPPPLVGI
                     WARWPYFHNNSVPSLCAVLSAQKDRPDFYYSGEAQVAETDFDVECNGYPLGAATPNSW
                     KDLKHSYDTHRPGMGNQGHDEGIFLEKGRELLSPRQKRALIQFLQTL"
     gene            complement(11746..12885)
                     /locus_tag="VIG33_07300"
     CDS             complement(11746..12885)
                     /locus_tag="VIG33_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8270680.1"
                     /translation="MVTKSMAISLTIVSLFASLTMAADPQTSVMGIPTTGSMNVHDPV
                     GWFPFIGISGGYMSPDDDLRTEGSPGDLKIIGSYFSENKNNVIDIGLGFMGDSFTQKS
                     DTQNNFISGGVAELAWRYNWSNRWQFGVAADTFIGGGDRYGSTDPNLTSFGGLQLIKE
                     MPIKNSNMFRIGLKGLTSLSIPSKSINTVMLDLQWGFGNEQKGPEVGANARPSGSDIG
                     APTGVSPDLESGGALTAGATLRRVEVDPADNSLIVRNDAHMQFDSGSTTLSSMNADYI
                     ERLGRALAQRQDLFDRVEVVALVDQSGSSRANVNTSRARSQQVSRHLTQGGLSKNKIQ
                     TTWKRSSDPMYKSLLPEEMQQSNRVDLRFRGVKDPGALQELLANP"
     gene            13211..13519
                     /locus_tag="VIG33_07305"
     CDS             13211..13519
                     /locus_tag="VIG33_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270681.1"
                     /translation="MGFNQQYDVPGLFDFLTNTPEAGLRKMLVDNKAFTDVHLSLLMK
                     VVRACDVAKFCEHVEKTDFPKVKLGPAEIKLKEKFWNDSFTWLATKGLLNPAQKKHAA
                     "
     gene            13523..>14287
                     /locus_tag="VIG33_07310"
     CDS             13523..>14287
                     /locus_tag="VIG33_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY8270682.1"
                     /translation="MFAYLAFENFSQELEKELRNKKAISDRLFVSETLQKCLWAQHTW
                     LNCEIIEINSVGDAVKALKAKGKWWALYSFDHHRRAQLIQEQLPKYKVPPLKFLGELP
                     SPAMGAWTLLEKNKILLSAETTSPFPLGEIHFQENKEMPPSRAYLKLWEIFTVHKIVP
                     KKGSVCLDLGSCPGGWTWALQQAGAQVISVDKAPLDPKIAKLPNIEFRKKDAFALKPQ
                     ELGPIEWLFSDLICYPPRLLELVEDWRASGLCKNFVC"
BASE COUNT         3946 a         3484 c         2933 g         3924 t
ORIGIN      
        1 tctaataagg taaaatgaat atcgacttgt tcttttgcag aaaccacctt cacaggaata
       61 ttcactaaac cgaagctgat acttccactc caaagacctc gatgcgggcg aggcgtttta
      121 ctggccaatt tacttttcac gttttccacc cctatggctg cttctctacc tttatttggt
      181 tattcacatc tctaactcca cgagttttat aaatggcttc ctcgacccaa tatctcatct
      241 tgcgttcggg gactgttcca gttaaaaata cttccccttc tttaacttcg acttcgaccg
      301 cagaggcgtc aatctcggaa tccaaagtta aaactttaac aatttcctct tgaagacttt
      361 catccgaacg ctgataattc ttgggacctt ttccttgatg aggtcggtcc acccatcctc
      421 gggcgacaga gccataaact ccgtaagtcc gattatatcc agtttcattt ccttccttgg
      481 aaccccactc acgacttacc agagatttat catactgaac tccgacactt tgatcgtcag
      541 catgcaatgg atttttttct cgatgtattt tagggatcac ggaagcacct cttatttttt
      601 attttaatgg ttaatatagg attcaaaagg ctcctctgac acatctcttt gaccttgctc
      661 caatatgcga tttttcaact ttaaaaagtc cctgcccaat tcccgacgat cctcgacatc
      721 agtcattttt tgaaaattag gcagtacatg ttcttcttct tctgaaagat gactctctat
      781 caattcggac ataatcttca cccgcgcgga ccacaaggca tcatctgcgg ttcggtgagc
      841 ttcttttaac aaatggtccg ccagggcatg ctcgatatcc ccttccatcc cattttcacg
      901 ggttttatca tcgtgcttca ggtaaacgta gagagtttgt tcttctggtt ttatatgact
      961 gataaaaagc agagcaaatt cttcaaatgc cgcttgccga tcctcaatgc tattctcgac
     1021 atttttgaga gtttttaaaa gttttctcaa gggcttatga tcttgaagta taagtttaac
     1081 aatatcttct tcccctatat tttcattttc cccggtcatc ggcctcgttt caggcttctt
     1141 ttttttgttt tttttatcct tgttatccac cttcatatat aaactcctct aggttaggac
     1201 ttaatattta catatttacg gccaagcaaa atgactgcca atatgtatat tgacgtaaac
     1261 cggaagactg cctgtttatg ttctgcaaca aatggtcaaa ttgttatttt gatgctagcg
     1321 tattctcatg gaaaaattgt cccgacgcga acaagaattc atccaaacag cagctcaatt
     1381 tcttgaacac cctggtttta tgattcaact ggcaaatgct tttggaaaac ctttggacct
     1441 tttgcaaaag actcttcccc aaaatgtcca agaaaaaatc gcaagccttg tggaaaaatc
     1501 cctaaaaaag accttggaaa tttccatttc cactttgccg aaagccgagg atccatcttt
     1561 gccaagacaa cctttactac aggcaagaca tcacactttc gccgctgccg ctctgggagc
     1621 catcggcggc ttttttggac ctggcactat tgccattgaa cttccgatca ccacaggaat
     1681 tatgtttcgc tctattgcca acaccgcaca aagtttcggc gaagatctcc aagactccca
     1741 cgtgcgtcta gaatgcctac aaatatttgc catgggctct ccgcagagca aggcagatga
     1801 tgcgatgaag tccgcctatt tctcccaacg attggccttt tactccttta ttacaaaggc
     1861 ttccgaaaaa ggagccgcca ctttgctggc tcgattcatc acccgagtcg cctcggagta
     1921 ccaaattgtg gtcgcggaaa aaattcttgc cgaaaccctt ccaattttag gtgccgccgg
     1981 gggagccgtt atcaattcct cttttaccca ttacttcaat caaaccgcct actaccattt
     2041 tggtcttcgc tatctcgaaa gaaagtacgg tttagaaagc gtccaacagg tttactttga
     2101 ggcttttaaa aatttcagat caaaataaac agacttctta tgaactgccc gcttttttat
     2161 ttgcgacctc ctggctctga gtcttaaaat aaaaaacaag gtggtgtttt tatggcgaaa
     2221 aatcgacgct gggtgattaa agcgggtagc aaaatggttt gtgatggagg gcctctcctt
     2281 atgcgtgctt ggatgcaaca agtggcactg cttcgtaaaa agcatggcat tgaagttatt
     2341 tgggtgacct cgggggccat cgcctgggct gtcgcgcgca ctaactttaa aaaaaccaaa
     2401 cgcaccttgc cccagaaaca agccttgagt gcgattggtc aacccttagt gatggatcaa
     2461 tacaatttag ctctgcaatc agcaaatctt ttaggctcac aagtactgct gaccgcgggt
     2521 gacatgaaag attctgagcg ccgaaaaaat ctgcagaata ctttaagtga gctgctgcgc
     2581 tggaaggtga ttcccatttt aaacgagaac gatgcggtgg ctacagaaga aattcgcttt
     2641 ggcgacaatg attccctagc ttctaaagtc gccatcatga tgaaagcgga gcggctgatt
     2701 ctgatgaccg atgtggaggg gcttttcgac gctgatccga ataaaaactc caaagcaaag
     2761 ctgattccct atcgccgccg cattgggaaa gcagatttcg cgttggcgga tcgcaaaacc
     2821 gtttccaagg taggcactgg cggcatgtat tcaaaacttc tcgctgcgga taatgccaat
     2881 cgcacaggca ttaccactca cctcgtccgt ggagatagtg cgaataacct tttgcaaatc
     2941 gccaccggta tctccatcgg aactcaattt ggaggacgcc atgaactcga ataatcaact
     3001 cagttatcag atcaatgaag aaatgctaaa gtcccttcgc ggtgctgcgc gcacgcagag
     3061 aaacctttcc gccatcgaaa aaaataaagc gcttcttaaa ctggctgagg atcttaccag
     3121 atctgcttca gatatatttg ccgctaacca aaaagacctt caatgtcttg cagcagacac
     3181 aaccagggcc tttcgcgatc gtttgacttt gaatccagaa cgcattgaag gaatgatgga
     3241 aagtcttcga caggtcgccg ccctgccgga tcctgtcggc gagctgatcg aggatcaaac
     3301 tcttaaaaat ggcttacaac ttaaaaaaat acgcgcgccg ctaggtgtga tttttatgat
     3361 tttcgaatct cgtcccaatg tgattttgga agccttctct ttggccttta aatctggcaa
     3421 tgtgatctta ttgcgtgggg gttctgaatc gcgttttacc agcgaggcgc tttaccgcgt
     3481 gatgcgcgaa agtcttcact ccagtggttt cgtgaaaact cctttttatg gtcttgaaga
     3541 ttatgaccgc gcattagtgg aggccctctt aaaacgtaaa gacttcatcg atattgtggt
     3601 tccacgcggt ggcgaaaagc tcatcgattt tgtgcaaaaa accgctctga tgccgattat
     3661 aaaaaatgat cggggtcttt gccatacttt tgttgatgag gatgcggatc tggaaatggc
     3721 ggtaaaaatc gtcgtcaacg ccaaagtcca acggccagga gtgtgcaact ctttggaaac
     3781 agttctcgtg catgaaaaaa ttgcgggtga ttttttgcca aaactctatc gagccaccga
     3841 agataaaaaa ctcgaatggc atgtcgataa gatcagccaa gaaattcttc tgggaaaacc
     3901 ccagataatc ccagcccagc cagcggactg ggataccgag tatcttgacc tcatcataaa
     3961 ttgccgcgtg gttaaaaatc ttgaagaggc tctcacccat attgaacagc atggctctaa
     4021 acactctgag gccattgtga ccaagtcgga gagaaaagcg cgtcattttc agcaagaagt
     4081 ggacgcagcg gcgatctatt ggaatgcctc cacgcggttt actgatggct ttgaatttgg
     4141 cctcgggggt gaactcggca ttagcactca gaaacttcat gtacgaggcc ctgtgggcct
     4201 acgagagctc acaagtcccc gttggatcat tgaggggact gggcagatcc gcacgtaaca
     4261 aggatttatt tcaaggtcaa cgcaagagga accttgataa gttaagaaaa tcaggtaaaa
     4321 caaatttatt ttaacaatta cgttaataaa aagtggagaa atgaatgaac catgagtttc
     4381 gcaatttttg cgaattctat aatttttatt tatcccagca ttccaatcga gagtgccgtc
     4441 aactccactt ttttggaacc tctttatttt ttatagcagt cggctatgct ctggtaacac
     4501 aaagctggct cttgattcct ctagcggggg ccatcggtta tcttctcgct tggagtgggc
     4561 attttttctt tgaaaaaaac cgtccggcaa catttcatca tccgtattgg agcgccagag
     4621 caggacgact tatgtatttt caattattaa caggcaagct ttcgctcaaa taatttttct
     4681 ttcaatttca ttcaaataaa cagatatttt taaataaacg aaaatatttt ccagacttag
     4741 gaatcgatcc tagccttatt atcaaaaata ttttgtcact ccttgaagct ccccttaaag
     4801 taacaggctg tgagcctatt catgcactcc catctttgaa cgttcgagcc gctcatttgt
     4861 tttgtgattt cgtaccaata accattttca taggagaaaa tatggcctca gatgttcgaa
     4921 actggcgtag cattgcattg ttagccgtta caatggcatg cctggcacca aaagctcagg
     4981 cagccacttt gctttttaaa actgactttg gagcggatac gaagctctat cccccagtca
     5041 tcgcttatag caccgctggc tggcaagata ttgccggtac taacctcact acgggaagat
     5101 tagggcctat taatctttgg ggagcgacag cttcaggctt tgatatgatc gccacctccc
     5161 cggttagcct tcccactctg ggaaattact caaccaatga aattcagcaa gtgacgggac
     5221 cgaatggaac tccagtgaat gcgctctttc aaaatttaaa agtcactgga ggtgtcgcac
     5281 aagatgcctt tatgattaga agggcctccg ttgatcgtgg agacaagact gatacgggcg
     5341 atgtttacta ttcctattgg ttcaagtttc aatcagattt acactctcaa ttaggcaccc
     5401 agggagccaa caacaataat tggcgagtga tgtccgagtg gaaaaccggc ggtctcaata
     5461 atacttatgg cggcgattat cgtatcatca ctgtcgtggt ccaagatccc aacggcaatc
     5521 tttcatggag cacccaggga gacagtaatg caaacgggat tcccatgcag gttttctgga
     5581 agatccctaa taccaccgtc ccagtccccg taggaaaatg gttcaaatat gaagtgttct
     5641 ggcatcgatc tagcggcagt gacgggcgct attgggcagc tgtcaatggc caagtgatag
     5701 ccgatcgtaa agggcccaat atgggcgctt acaatcttcc aatcaatcgc atcatgcttg
     5761 ccaataacta cactggagga agagctccat gccagcaatg gttaactggt ctggaaattt
     5821 ggaatggttt cccatgcggc gtcggtgtca gttgctacgg agctccgact ccaactccaa
     5881 ctccgactcc gactccaact ccgactccaa ctccaactcc aactccgact ccaactccga
     5941 ctccgactcc aactccaact ccaactccga ctccaactcc aactccaact ccaactccga
     6001 ctccaactcc gactccaact ccgactccaa ctccgactcc aactccaact ccggccccaa
     6061 ccccagttga taacgttgct ccactgactg cggttacatc accaatcaat ggctcaaagg
     6121 tgtctgcaaa gtctaatatt actattcagg caactgcttc agacaatgtt ggagttaaga
     6181 ctgttacgtt ctacataaat ggcaatttaa aatgcagcgt ctcgacagct cctttccaat
     6241 gcaactggag agtgccgcgt cctcgaggaa gaacttataa cctaaggaca agggctacgg
     6301 atgccgctgg caatgttgga gtttccagga gagtgactgt aacttcaaag taacctatta
     6361 aaaatataaa atgtgtccaa aaaaaagaga gcctcgatca gaggctctct tttttttaca
     6421 agaaaaatat tccactagct gaatttctta aggcaaaact agaatagtat tttgaagcca
     6481 attttggatt tcttgacgat ctaaacgagt gtaaatactg gtgaccaaat gaggttttcc
     6541 agtggccgga tcatattttg ttttcatcgc cgaaaccacg gcaatttgag ttaactttaa
     6601 gggggattct tcagccacca acatgagcgc gccacccgaa tctccccagc acactccgcc
     6661 attgccctgc ctatctgtcc aaatggaatt caattctaaa gtggaatcca acggatttag
     6721 cagaacatca gccatacgaa aaacccccac tccttttgga atttctcccg gaggaatgat
     6781 ataatcagca cagccttgac ccacgagttt aaactttttt tggttcaact ccaggcttaa
     6841 ggctgaagga gaaattaaat gaactacttc caaatttggt aacttactgc gaaggccttc
     6901 ttttaaaaca atcacagcta gatctggctt tggcagacga atcacggaat gagtttttcg
     6961 cgtgggagcc ttgtaccctt gttcctcgag aggaggaaaa taaacctcat agtcagcttt
     7021 gtgaacacaa agcttttgca agttcgtata atcttgaata caaatttcag ggtctatctt
     7081 tgcatattca ttcagtaaaa aacagtgtgc tgctgtcagg attttaccat ttcctaaata
     7141 agtcgctgaa catggatctg gtgctaaacc cttaaggcca aaattcaatg aaacaaaggg
     7201 cgagggaaga ttttcatttt caattaactc cacaccttga acaacagcaa atccactgat
     7261 tgaataaaag aataaattaa aaaagaacag aggaattaaa atcctttttt tcattgtaga
     7321 tcccctttca ggcttaacag ttaacatgac actatgcgtc ttggtatagg tcagtttgtg
     7381 aatttacaca agaattatgt aaatcttcta agaacttcat gaaagctttg taaaaatggc
     7441 aagatcaaat gccaatctcg ataatcgaat cattaaaaat aataatgagc taaagcccaa
     7501 gccactgtat tgtaggcatt tatctccgcg gtttcactgt aatccacctc cgcgctaaat
     7561 tcaaaatgcg gacgaggata aaaaagcacc cccactcctg aactattcac ccgcgtacgc
     7621 agttgagtga aatccaaata cgaaaactga tggagcaaat aagagtgaaa gcccttggtc
     7681 cactcatact gcaaacgact gtaagtcacc actccctttt caacaacctt cgtggcgccc
     7741 tctagactct tttcttgata atcatattcg cccagaaaaa cccatcgtga attaaagccc
     7801 cataaaatct gaggtcccaa caaatagcgt tgagaattgc cattttttaa gtaagcaaag
     7861 ctccccgata ttttgtgcgt gtcgcccaag ttatagccca cctgtaaaga gccacctttt
     7921 tcaagatcca cgctgttgtt gtctttacgg ccaaaactca aagcaaaaaa cgtattccat
     7981 ttttcgctgt tgcttccaat atcaatgtta taactctctt ggccttcatc aaaaccaaga
     8041 ttgcgtttaa taaacgacgt gtgatccgga atatttaagc cataaggtaa aagatatttc
     8101 ccgccacgac tgtagacttc atcattccaa ttaaagagcg cgtaaaaatg atgagagacg
     8161 aattttcctt gatccggcgc ttgactagga cctccctgta ggccaaagct ctgaacgtac
     8221 cagatttttt caaagttcaa tcccagatcc agatctgttt gcatgggaaa gcttgaagtc
     8281 gccgtggctt gcgcattatc cacataagtt tgaatgagac gaatatcgcc accgaatttc
     8341 aaccacgctg gaggctcagg taaaaagcca tgtcccggtt gttcctcttt aggaacggcc
     8401 caggtgctga gaacctctga agaaatggag cgaccataat ccgtgagcaa cccaccaccg
     8461 ttgggggcga catgacaagc cgtgcaattg acataaccca tgcgaatcat ttcagggtaa
     8521 gcataggcag aacttgaaag agtccaaatc acaaatccca cgaaaatttt tattgagcgc
     8581 atacccaatc cagatcaaca gtattttgca cttgatcagt cactttaatc cctaagtaga
     8641 caggaatctc gatcttgtaa tccgtaatat tcaactcaaa acgaatttgc gcatgccctt
     8701 tgcctttttc agcagtggca tcaaaatcgc ccttcactgg atgttgtact ccgtgaagat
     8761 tcaacgtgcc ctcaaaaggg gcctgggtca ctttcacgct ctctttgcaa ggatcttgcg
     8821 ctagtgccac gggctttaac tcaagctccg catttttgaa tttaccgctg aacttaccag
     8881 tttccaaata tttttctttc atgtgattgg tgcgcagctc aatgcccgta tccaaagact
     8941 ctaggttaaa agtgaatttc ccagagagtt gtttttcttt ccattgagct tcacttttaa
     9001 gatcatctcc tttgccccga atcttaatag cgctgggctt accaatcgct agaaattcaa
     9061 tatgtccctt gatgactggc agtggcgccg cccctgcatt cacccccaac cccatcatcg
     9121 taaaaatcgc tcccagtgct gttttcatga tcctacttcc aatttaatct tttgctccgt
     9181 tcaagatcca actttttata gcatcaatct cttcagcgga caaagtcatc cccgttttta
     9241 gcggcggcat cacttttctg tcagtgcggg taatcgccac atagagagaa ctgtcgggcg
     9301 gactgccagc cttcactaat ccattggctg gattcaccac ccagtctttg tccgtcaatg
     9361 gaaaatcttc agccttttca ccggcctgat ggcatacgat gcatttcggc tcaaaaacat
     9421 ttttgtaaat atcattccaa tttgcaccaa tcaccactgg atttacagga gggggcgtcg
     9481 gagtaggctc cggttccagt gccggctttt catcatgcgg agcgccagcg tcgagccaca
     9541 ttttcaaaac atttttttca taagcgccaa gaggcccccc tctgcttaga ggtggcatac
     9601 tgtcttcagt gaaaatgtcg atttggatat ccgcaagatt cactaagacg ttatcatagc
     9661 tctcaagatt cacgccgcct ttattgccac cagccaagct atgacactgg aaacattttg
     9721 gctgaaaaac cttgtcattt acaaatgcat aatcgatcga tgaaggggca actgtatcct
     9781 cgggctgact ttcaactttc gacttataag agttgcaggc aagtagggaa aatgcggaga
     9841 tgaagaaaaa ggattttaga ttcatgaaac cctccaaaag tcgctcaaga atatatgcta
     9901 tttaatattt ttccacctct attcgtcctg aaaaatataa atatttcagt ggtgtctaaa
     9961 tattcccctt atatcgtcct aggcggaggt ccattatgca cctcctcttg gtggagccat
    10021 tttgcataaa caaaagatcg gcttattcta aagacctcac ggaggagagc gaatgaaatt
    10081 tttattgatg ggactttttc ttttggtaag cttgaaactg catgcggcaa ttcctgactg
    10141 gagtgaaaat ccccccgccc ctgctggcgc aaatccctat caacttccaa gcgatctctt
    10201 tccgcaatat cgcgacgccg gaaaaatcca tgcgcagatt tatcccgtca gcatcaccgg
    10261 gatgctgcct ccttacgaac ccatccgtca cctcatcgaa gattcttcgg caaacccact
    10321 gcgtaatttt ttgaattcgt tagtgcgcgg agtttcaggg cttcatagca tgcaagatgt
    10381 tttcacgagt ctaggccttc atgattatcc gcaagcccat gatcagggag tttatgcggt
    10441 tccttttcct caaggagtgc gtcctgatta caaaatgggc tttggtttta tcgaaacttc
    10501 tcaaggaaag ggatttagct ttagctgcgc cgcttgccac tccgctcatc tttttggaaa
    10561 aacagttctg ggaatgacca atcgttttcc gcgcgccaac gagttcttta ttgaagcgag
    10621 caaagccacc catttcgtgg atccggattt gtttaaagtc tatgccaatg ccactgaagg
    10681 tgaaactcaa atggtgaagc agctcaaaga aaatatgaaa tatgtgggtt ttaaaaaacc
    10741 tctcacgctg gggctcgata cttcgttggc ccacgtcagt ctttctttaa atcatcgtaa
    10801 caaggatcct tatgccacct atagcggcgt ctttgcttcg aaccctcgtc ccgatcctat
    10861 tttagattct tcgcccgcgg actcgaaacc cgccgtatgg tggaatttaa aatacaagaa
    10921 tcgttggctc tcagatggct cggtcgtcag cggcaatccc atcctcacca atcttttatg
    10981 gaatgaaatc ggtcgtggtg cggacctcgt gaagctggaa ccttggttgc aacaaaatga
    11041 aaaaatcgtg caagaaatta ccaccgccgt attctcttcc gaagcgcctc tcttcactga
    11101 tttttttccc gctgaaaaaa tagatatcgc aaaggctaaa gagggcgagg tgatctttaa
    11161 ccagacctgc tctcgctgtc atggacacta tgacaaggcc tggaaccttc ctcatgaaag
    11221 tgagctaagt ccaattgaga aattaaagac cacggcggtt cgctatccca ctcaaactca
    11281 ggtcaaagat gtgggcaccg atccatatcg ctatcgcggc atgcggtctt tagaaaaact
    11341 caatgatctg gctatttcac ttaagaatgg catcctgatt cgcgcccaac aaggttatgt
    11401 cccgccgccc ctcgtaggaa tttgggctcg ctggccttat tttcataaca actccgtgcc
    11461 gagcctctgt gcggttctga gtgcacaaaa agaccgccct gatttttatt attctggcga
    11521 agctcaagtc gctgaaaccg attttgatgt ggagtgcaat ggttaccctc taggagcggc
    11581 cacaccaaat agctggaaag atttaaagca ttcctatgat acacacaggc ctggcatggg
    11641 caatcaagga catgatgaag ggatctttct ggaaaagggt cgagagctcc tttcaccacg
    11701 gcaaaaaaga gctctcattc aattcttgca gaccctttaa tgaacttacg gattggccaa
    11761 caattcttgc aaagcacctg ggtctttgac acctcggaat cttagatcca cccggttgct
    11821 ttgctgcatt tcctcgggca acaaagattt atacatggga tcggaagatc tcttccaagt
    11881 ggtttggatt ttatttttac tcaagccccc ttgagtaaga tgtctagaaa cttgctgtga
    11941 tctggctcga gacgtattta cattggctct actagaacca ctttgatcca ccagtgccac
    12001 cacttccacc ctatcaaaaa gatcctgtct ttgagcaaga gctctgccca gtctttcaat
    12061 gtagtccgca ttcatactgc tcaaggtcgt actccctgaa tcaaattgca tatgagcatc
    12121 atttctaact attaacgaat tgtcagcggg atccacctct acacggcgta aggttgctcc
    12181 ggcggtcagc gcccctccgc tctctaaatc cggtgacacc ccggttggtg cgccgatatc
    12241 tgagccactc ggtcttgcat tcgcccccac ttctgggccc ttctgttcat tgccaaagcc
    12301 ccattggaga tcaagcataa ccgtgttgat agatttactc gggatactta aactggtgag
    12361 acccttaaga cctatgcgaa acatattgga gttttttatc ggcatttctt ttatcaattg
    12421 caaaccacca aagctagtca ggttgggatc agtggagcca taccggtctc cgcctccgat
    12481 aaaagtatcc gcagcgactc cgaactgcca gcggttgctc cagttatatc tccaggccaa
    12541 ctcagccacg cctccggaaa taaagttatt ttgggtgtca cttttttgcg tgaaactgtc
    12601 ccccatgaat cctaagccga tatcgataac attgttttta ttttcactaa aatatgaacc
    12661 gataattttt aaatcccctg ggctcccttc tgttctcagg tcgtcatcag gggacatata
    12721 gccacctgat ataccaatga atggaaacca tcctactgga tcatgaacgt tcatggaacc
    12781 tgttgtggga atgcccatta ctgaagtttg cggatctgca gccatagtaa gcgaagcgaa
    12841 cagactcacg atagtgagtg aaattgccat tgattttgtg accataaagt cctcccttga
    12901 atggtcccat ttataaccaa gtcctgtggg agagccgtga aagagttgtg acaatattaa
    12961 gatttaagtt tagagagtaa gtaaagaggc cccgctagca tccgagacaa agcgaaaagt
    13021 ggcaaagcac ataactgcca aatatatact ctagacctag actacagatc gatctgttcc
    13081 gaaaaatgaa aggtccagac ttctccaagc cccgtcaata gacaagaagt ctccattaaa
    13141 acttaatcaa ttaccgaagc atccgataag cttaaagaag aaagtaaaat ctaacaaagg
    13201 ggtatgattt atgggtttta accaacaata tgatgttcca ggccttttcg atttcctcac
    13261 taacactcca gaggccggac tcagaaaaat gctggttgat aataaagctt ttacggatgt
    13321 gcacttgagc ctcctgatga aagtggttcg cgcttgtgat gtagccaagt tctgcgagca
    13381 cgttgaaaaa acggattttc caaaagtaaa gttgggtcct gccgaaataa aactcaaaga
    13441 aaagttctgg aatgacagct ttacctggct ggcaacgaaa ggtcttctaa atcctgctca
    13501 aaagaaacac gccgcctaat ccgtgtttgc ctatctagcc tttgaaaatt tcagtcaaga
    13561 gcttgaaaaa gagctcagaa acaaaaaggc gatctctgat cgtctttttg tttctgagac
    13621 tttgcaaaaa tgcctctggg cccaacacac ctggctcaac tgtgaaatca ttgaaatcaa
    13681 ttctgtcgga gacgccgtca aagccctaaa agctaaaggc aaatggtggg ctctttattc
    13741 cttcgatcac catcgccgcg cccaactcat tcaggaacag ttgcccaagt acaaagtgcc
    13801 accgctgaaa tttctcggtg aactcccctc tccagcgatg ggggcttgga cactgcttga
    13861 aaagaataaa attctcttgt ctgcggaaac aacgagtcct ttccctttag gggaaataca
    13921 ttttcaagaa aacaaagaga tgccgccttc gcgggcttat ttaaagctct gggagatttt
    13981 taccgttcat aagatcgttc caaaaaaagg ttctgtctgt ttggacctgg gaagctgccc
    14041 tgggggttgg acttgggctc tgcaacaggc aggcgcacag gtcatcagtg tggataaagc
    14101 cccgttggac cccaaaattg cgaagctgcc aaatatcgaa ttccgcaaaa aagatgcttt
    14161 cgccctcaaa ccgcaggagt tgggccctat tgagtggctc ttttcggacc tcatctgcta
    14221 cccgccccgc ttgcttgaac tcgtcgaaga ctggcgagcc agcggccttt gcaaaaactt
    14281 tgtctgc
//
LOCUS       DATBTE010000198         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_25579, whole genome shotgun sequence.
ACCESSION   DATBTE010000198 DATBTE010000000
VERSION     DATBTE010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25579"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1725)
                     /locus_tag="VIG33_07315"
     CDS             complement(<1..>1725)
                     /locus_tag="VIG33_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270683.1"
                     /translation="SLVLPEAGVFHIRIYFESALVQKISRKRVSGENDTPYANYLEDH
                     AKGLEIDILPQQEFMLWSIYEEQKNIWSQPIPKPYLWQIHPGAKGRVIARIRSRNAGW
                     SLASIGLSGRNTQHSSEQGLPSLDVKIISGPLFYGLQEFKCYTLGSKTSGRDINLEAF
                     LSDIGLKTPREDFVAVSMNGKKMGLMEVHEGLGENFFEFAQIQEGAVLGYDADTAFSE
                     YDSYRLEQKSYFKKSRYSTKGDDIATEDFFKKLCLDSFAKGTAFAYAFFGVHGLNQTD
                     LRFHLNQRLNCHELFIRDINSGVAAIGTEINNANFFTSLMYFSSFTPEWRPQMVSHAS
                     FTVLKNESLISKQPMWYWSQHPSFLFFMENKEHREIFFQWMNFFSSESIRSRLEERLV
                     LLKNSLNILAQTNDTVFANHISPSPYKTFPVEKLWRQTIPVSSQMMNLAENIKSVSQQ
                     KILLTQDDLQNFYGRNQSLGIYKELESLKEIPGASGPIATFLYRKESPQETHMIFLVR
                     GIIGPNYKIELADLKKKKFFADNIQNFLGSAFTLDQWHLYTNELRNGEGYSLAYFVIP
                     KKKELQYIK"
BASE COUNT          524 a          367 c          322 g          512 t
ORIGIN      
        1 cttgatgtat tgaagttctt ttttcttcgg tatgacaaaa tatgcgagag aataaccctc
       61 accgtttctt agctcgttag tataaagatg ccactgatct agcgtgaagg cagatcctaa
      121 aaaattctgg atgttgtcag caaagaattt tttctttttt aaatccgcaa gttcaatctt
      181 atagtttggg cctattatcc ccctgacaag aaaaatcata tgggtttctt ggggcgattc
      241 tttgcggtaa agaaaggttg cgatagggcc cgaggcaccc gggatttctt taagggactc
      301 gagttccttg tatataccca acgactggtt acgtccgtaa aaattctgaa gatcgtcctg
      361 ggttaataaa attttttgtt gtgaaacaga ctttatgttt tccgccaaat tcatcatttg
      421 tgaagataca ggaatagttt gacgccacag cttttctacc ggaaatgttt tataaggact
      481 tgggctgata tgattggcaa aaaccgtatc gttagtctgc gcaagaatat tcaatgaatt
      541 tttaagtaga accagacgct cttccaaacg agatctgata ctctctgaag aaaaaaaatt
      601 catccattga aaaaaaatct cacgatgttc tttattttcc atgaaaaaaa gaaaacttgg
      661 atgctgggac caataccaca tcggctgctt acttatcaaa ctctcatttt ttaacacagt
      721 aaaggatgca tgacttacca tctgtggtcg ccattctggg gtaaatgacg aaaagtacat
      781 cagcgaagta aaaaaattgg cattattgat ttctgtgccg atagcggcaa cacccgaatt
      841 aatatctcta ataaatagct catggcagtt taagcgctga ttgagatgaa atctcaaatc
      901 tgtttggttg agcccatgaa ctccaaagaa agcataagca aaagcagtgc ccttcgcaaa
      961 cgaatcgaga catagctttt tgaaaaaatc ttctgttgca atatcatcgc ccttcgttga
     1021 ataacgtgat tttttaaaat aagatttttg ctccaatcga tagctatcat attccgaaaa
     1081 agcagtatcc gcatcataac ctaagacggc cccctcttgg atttgcgcga attcgaaaaa
     1141 gttttcaccc agcccttcgt gaacctccat caagcccatt ttttttccat tcatcgaaac
     1201 cgcaacgaaa tcttccctag gggttttcaa tcctatgtcg gacaaaaatg cttcaagatt
     1261 aatatcgcga ccactggtct tggacccaag cgtataacac ttaaattcct gtagaccata
     1321 aaataaaggt ccgcttatga ttttaacatc caatgaaggc aagccttgct cagaactatg
     1381 ctgcgtatta cgtcctgaaa gcccaatact agctaaactc caccctgcat tacgtgatct
     1441 aatgcgagcg ataacgcgcc ctttagctcc aggatgaatc tgccataaat agggttttgg
     1501 gatcggctga ctccaaatat ttttttgctc ttcatatatt gaccacagca tgaattcttg
     1561 ttgtggcaat atatctattt cgaggccttt cgcatggtct tctaagtagt ttgcataggg
     1621 agtatcattt tctccagata cccgcttacg gctaattttc tgcaccaaag cagactcaaa
     1681 ataaatacga atgtgaaaaa ccccggcttc gggcaacacc aaaga
//
LOCUS       DATBTE010000199        15916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_640586, whole genome shotgun sequence.
ACCESSION   DATBTE010000199 DATBTE010000000
VERSION     DATBTE010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 15916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15916
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640586"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VIG33_07320"
     CDS             complement(<1..983)
                     /locus_tag="VIG33_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8270684.1"
                     /translation="MLEKGHSLIRVEMLNWGNFTGYQEAVFRDQMNLGPLFAPPPAST
                     ILGVNGSGKSTLIDGIMIGLLPFDNLLKLGVTNDYEKGAAGGRSMTDYVLGKYASTTG
                     KENKNLSNVYNRESGCSVLQLCLQNNHSPASYISAGLIWWYSNYQIQDRLLYITQKDM
                     RVKDYCGAAMNLPKNGKKFKETLMEKYPSSQIFDTAQSYFSTLSTLLGGITKEDLKIF
                     NRAFYVKSIENIDHFVRENMLIEEPNENLEILLENVANGNEITQQIAKCRDKIQESEK
                     ILNEFSHLQKVLDKLEQLEKERSFLRLFQQWEPLKKNEEETTTGQFHKEKL"
     gene            complement(986..1600)
                     /locus_tag="VIG33_07325"
     CDS             complement(986..1600)
                     /locus_tag="VIG33_07325"
                     /inference="COORDINATES: protein motif:HMM:NF025205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HEY8270685.1"
                     /translation="MISNHNIIIAGCLRRLLKNRFLLQKKSREWFRNIVDCQVQLSSH
                     IEALGAHLIINESLGVAYLAPGDAELEEKIGFQLGTTLTLKKISTMILLSLTKRRLDY
                     QNAPENEICAISRQSIKDEILPFLSHIEKYKEDRAVERELKDSIRELKELQVLFELPE
                     SEDVFEISPVCNILLPFEETQRTRAFIEAYLSQSSSIEDAPEEN"
     gene            complement(1597..3096)
                     /locus_tag="VIG33_07330"
     CDS             complement(1597..3096)
                     /locus_tag="VIG33_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270686.1"
                     /translation="MAKRSEIIHRESLESLFATHPYLLVFKKLDWDEYIGLLAHIYDL
                     LEEENTRVPYDVLKAILIQYYSVKVQNVEVKVAEYFQIMIVELQVLKDSHDSIGKRYI
                     EATRMGKDFLKMMESLLSERRRFSGTGAETLLGALNGLLISGNEMTEEDALQHHKEKI
                     AAYQADIKRIKENGLKAAELLPFHHSNEALFSQAEEAAIHILSATEDVKAAVERERKV
                     LAENYLQQKQTPGANVRTIADFHLNLNNTEEFKSYNQAKELFSQLEGSPARHKHSNIP
                     RILSLLKSRNIVAEDVLKRSYLADFARHFDAADSSIKEKTRVQIRILQQQVAYALTTD
                     SRLIQENINAIFRLLYAHKENALSIIEGLNLELKIPCETDFGSVDTNEFHIQKEAEAV
                     SLVSEDLTAEELRFMMQALLKAEEDTIQRVLEDLKKHLQQYGPVALADYPHLHSLAAL
                     YALIEADTLDPAIEKIFQKEIDISLSLQNINIVLRKVPNYLLKHKAQKL"
     gene            complement(3311..3826)
                     /locus_tag="VIG33_07335"
     CDS             complement(3311..3826)
                     /locus_tag="VIG33_07335"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEY8270687.1"
                     /translation="MINLRKTLTEAHSILEQQGVSHALIGGFALAVYGQHRATVDIDF
                     LADGGKKELIKTSLLAKGFVLKHESPEVLQFSGMGFVDIVLANRPLSQEMLKQALKNT
                     ELGVYVVRPEDIVGLKIQAYKNDQSRELQDKADIQRLLKMPNLDFTLIKKYADLFQEW
                     PEIEKLRGQKS"
     gene            3911..4942
                     /locus_tag="VIG33_07340"
     CDS             3911..4942
                     /locus_tag="VIG33_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270688.1"
                     /translation="MRKFCYPHDIIENYCTPQVRIIEIIAPLYKENLSITEIAERTGF
                     KRTMVWETLKQHEKELKSQRVVPYERWRKGHKKTGVRPPYGFCFLQGEVVRDPKEYPT
                     LLLMHNLWTRGSGNMSILAAPFGIDSPFLVHLPLVCSDGEWRVALPQELAQNDLRNSA
                     AWFDKCDKTHAKKAGVERGTLFESLNYNNKLLVQNPSARRWIVFNAAGTNVSAATVEN
                     TELFWADQRTYWYCPSSKAEGDFLAAILNAPSLNLIIKPFQSVGLAGERDIHKKILEL
                     LPKYDGSNSTMAEISKLGAEAKTSVDAILATLKGKGIVTKRKTTRDHIQKTLNLIDKA
                     VKDLIQIKG"
     gene            complement(4932..5174)
                     /locus_tag="VIG33_07345"
     CDS             complement(4932..5174)
                     /locus_tag="VIG33_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270689.1"
                     /translation="MVTAKVIWFDRKKGLGEALDEMGRTLVLSALEITPSAKPIRLNP
                     KDEIVCSVRKSKTGFVAKQISKAEKINRKRPLDATL"
     gene            5299..5790
                     /locus_tag="VIG33_07350"
     CDS             5299..5790
                     /locus_tag="VIG33_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270690.1"
                     /translation="MNEVGSKGLLKVENNKANYNIISFGKRFENLAISVKSQIIGSKL
                     STFEANVKSGSKIFLHCGSQIWGLATVIGNYQYNQSKVWSNDLYPHRYNIKVDLLTLQ
                     PLHLSNGIYNVQLREQFGSGWAYKFIFSPRPLPGDVGEKIEKDLRTAPTCDLETFLSA
                     IKG"
     gene            complement(5991..6066)
                     /locus_tag="VIG33_07355"
     tRNA            complement(5991..6066)
                     /locus_tag="VIG33_07355"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6031..6033),aa:Gln,seq:ttg)
     gene            complement(6196..6519)
                     /gene="spoVG"
                     /locus_tag="VIG33_07360"
     CDS             complement(6196..6519)
                     /gene="spoVG"
                     /locus_tag="VIG33_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HEY8270691.1"
                     /translation="MKITEVKVFPVNEERLKAYVSITIDGSFVVRDLKIIQGPSGLFI
                     AMPSKKRKDGQFRDIAHPLNQETRAMIEDSIFEAYEKELKSMGATLVNLKRQKAPTTS
                     YDDEP"
     gene            complement(6764..8215)
                     /locus_tag="VIG33_07365"
     CDS             complement(6764..8215)
                     /locus_tag="VIG33_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY8270692.1"
                     /translation="MKKLFVALISLSLVTGLNLTYAQIPQKTPPAMKLSEPLPGNLFV
                     ELGRVINPAVVNISTLAIPKMNNLQRDPMLEMLEQFYGVPLQSRRQQMAPQGKPRPYG
                     LGTGFIIREDGLIVTNNHVIQGADGIQVQLTEKSDKLYDAKVIGYDERSDIALIKITP
                     DGKLPVAALGSSKDLEVGEWVAAFGNPYGHGHSMTKGIISSKGREIGEINKIPLLQTD
                     ASINPGNSGGPLVNTKGYVIGVNSAIDARAQGIGFAIPIDEVKAIIPQLEKSGRIEKG
                     YIGARLGDLDPSIDPTYLGLQEPEGAVITALEKKGPADKAGLRPYDIVTEFNGKKIKS
                     SMDLKDAVEDSKIGSSVTLKAIRERKPVSIKMTVLSKPNFAALNKNPGSSKSYSGQKV
                     PFNLGFTVSDLNDELRAEFQIPSEVMNPMIIRIERDSLAAMADLRVGDLILDINKKEV
                     TKALDVSKSLKKGTNTLRLARGNRLMIVTIDTK"
     gene            complement(8227..8475)
                     /locus_tag="VIG33_07370"
     CDS             complement(8227..8475)
                     /locus_tag="VIG33_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEY8270693.1"
                     /translation="MPEQMEASFSVLAMSIASSAVMAMGLAPDSPGGALKKDKNMARF
                     NIDLLVMLQEKTKNNLTAEEKKFLENLISDLQIKFVQS"
     gene            complement(8485..9318)
                     /locus_tag="VIG33_07375"
     CDS             complement(8485..9318)
                     /locus_tag="VIG33_07375"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY8270694.1"
                     /translation="MVANSTKVWVFILLSSLAFLILGYEIGERLGLFMGFLSSVALNF
                     FVFFYGESSLLTTLNSKQMQGQDPWGLDQILQKYSDHLGINPPDLYLIPLETETAFCV
                     NHLWRRGSVALSSGLVQKFSKEDLEAVMAYQLCNLNTMSSFMVGVTSALANALVGLGN
                     VLDHIWPPNYFLLAHQRHTPFLKMSSFLGWNLVKLANSHKKYYETDLQAAELIHDRFR
                     LAEVLWRLEGLAQTQPLRPPPCSHHLFIVNPEGYRQKLLLKSHPSIEIRLQKLMGYYP
                     I"
     gene            9446..10438
                     /locus_tag="VIG33_07380"
     CDS             9446..10438
                     /locus_tag="VIG33_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY8270695.1"
                     /translation="MSEIDVLALNAAIKQESQFVDKMMQEISKVVVGQKEMVEGIMMG
                     LLTGGHILLEGVPGLAKTLTIATVSKTISLDFQRVQFTPDLLPTDLIGTMIFNPKTGE
                     FNPRKGPVFTNIVLADEINRAPAKVQSALLEAMAEKQVTIGDQSYKLENPFLVLATQN
                     PLEQEGTYPLPEAQMDRFMFKIDVVYPTKGDELEILNRMGMNGKPSVNPVISKEDLLR
                     VSERADQIYVDNKIKNYIVEIVMASRHPAEYGLTRIVNLISVGGSPRATIGLFRAAKA
                     HAFIRGRGYVTTEDVKAVAYKVLRHRMILTYEAEAENIKTDDMIKEILTQVEVA"
     gene            10440..11306
                     /locus_tag="VIG33_07385"
     CDS             10440..11306
                     /locus_tag="VIG33_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY8270696.1"
                     /translation="MSLPPEVLKKVKMLEINTRKLVNNLFAGEYHTHFKGHGMTFSDF
                     REYVPGDDVRAISWPLTARTGKTYIKTFEEERELTIILAVDISGSTDFGTGEYFKGEV
                     MTHLAALIAFSAVKNNDQVGLLLFTDQVEHFVPPKKGRGHIHRILRDLLYYKPKSHET
                     KISTALTYLQGFLKKRATIFMFSDFFDENYDQALRMLGRKHEVVACVVNDKAEGQLPD
                     MGIVELQDPETGEVLTVDTSSAVFKRDYLREFKKRKDLRDKQLRLAQVERIDVNSNED
                     YVDPLVAFFKKR"
     gene            11315..12259
                     /locus_tag="VIG33_07390"
     CDS             11315..12259
                     /locus_tag="VIG33_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270697.1"
                     /translation="MAAVQCNLDIPKVPGLEDHQLTVGREFYLTCKGEWPKTLKKEAL
                     YFTLEKAGSEVKSETLKYHIKLLDFEFRSLENVDIKITSYLAGQHQFPNLILTDGERK
                     LELGPVQFSVQSVLEQGQQQKQEKVEPFGPMGPATIAVPVLYWVILFGTLVLMGTLGA
                     LKIWRHNQRREMLLRLKEHDSALSPLQEFHQSMRRLQRANPVFFGKEATSEELRQGVT
                     ELSRMFKVFISRRLKVPAFEWNERLIVNDLRRYHFFVHQEYSQKIRSLFLELHRAESA
                     NAKLTSHDVSQLAESLRKTLEGIEKLMDREQMAQKGDR"
     gene            12256..13269
                     /locus_tag="VIG33_07395"
     CDS             12256..13269
                     /locus_tag="VIG33_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8270698.1"
                     /translation="MTSWHSPWALVLLIPLLVILIWNYFQRKRKIPSLQFSSLKLIKT
                     IAPSVRTRLMIVPSLMKALAIVLAILALARPQEANTKVKKNVEGIDIVICLDVSDSML
                     IEDMKPTNRIEAAKNTIYQFIEKRTSDRIGLVVFAGESFTLVPLTLDYQLILERVKEI
                     TTAANANIKDGTAIGVALANAAGRLKDSTAKSRIMIFMTDGENNSGTIDPETALEIAK
                     GYELKIYTIGIGKDGPTKIPIYQRDIFGNKVKTYQPFDSTVNDELLSRMAKDTGGQYF
                     RASKEDSLEGVFKEIDSLEKTKVDVNKYTRYTELFPPYLQAALVLYLIGVLLAKTFLR
                     RVP"
     gene            13269..14330
                     /locus_tag="VIG33_07400"
     CDS             13269..14330
                     /locus_tag="VIG33_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY8270699.1"
                     /translation="MRFENPLAFYFLWTIPLLWILAVFFQKHVAKKMAKTIGPRLYPF
                     LTSSVSSYKRNTKRVLQSLVILFFVIALARPQLGQSMQEIKSEGVEIMFVVDVSESML
                     AEDVRPSRLEQAKTDLSRLLDRMPGNRVGVIAFAGSAGVLSPLSNDPNATKMYLESLS
                     PSSVSSQGTSFEQALKLAADAFKRGGVEEEDTVKVTRVILIASDGEDHEPGAIEAAKK
                     LAQQGVKIFTLAYGTEKGGPIPMKDGLGFWKSNKKDRSGQTIITSVKGDELRALAQAG
                     EGSFYFATYGSDYIKNVGEDISKLEKVEFESKMAIQYEERFQLFLVIGILLALIELFL
                     GERRASFRLWKGRYEVPPA"
     gene            14327..15163
                     /locus_tag="VIG33_07405"
     CDS             14327..15163
                     /locus_tag="VIG33_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8270700.1"
                     /translation="MSRSWGVLLISGMLALSAIFLSWSGLIEWPTLPHLKSLVLNRQG
                     NQQLKEQNYSSAFDAYVKTLEFEPFAPEIHLNLGLTYEGQQQPEKAMPSYENALKYAQ
                     SNEMKFASLFNQAQLLGKDKKVDEALEFYQKALSLDPTSKEVKTNIELLIQMQQQQKG
                     GQQDNKNKKDQKDDKDQKDNKDQNNSQNNKDQKDSKDQKDNKDQGKDEKDDKSKDQKD
                     QKEKSQYSQSEKYKPRPFKGQELSEGDVKKILGEIKQQEQKIRSEYNRKEVKEQPRDK
                     DW"
     gene            15147..>15916
                     /locus_tag="VIG33_07410"
     CDS             15147..>15916
                     /locus_tag="VIG33_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HEY8270701.1"
                     /translation="MTRIGNRFSLVLLLVSLAVPAWAKVSVQAVVDRNEMSVGDSFTL
                     TVSVNSEESVDIKEPRAPDMEGFELLNSWQSSSTSQRLVNGSGGMHFEVQRRQDFNYL
                     LAPRKKGTYSVGAFEVVVDGKVYHSQPMTITVGETGSAGPRKGRGGQLPGRRLIPPGF
                     GNMPGADVFEEMDRAEEELFNQLLQRRQQGGLPNGGIPGAPGGRFQAEPQYRSLPTNP
                     NEAFFIQVEVDKTDVYEGEQITASWYLYTRGQMESLDRL"
BASE COUNT         4455 a         3231 c         3752 g         4478 t
ORIGIN      
        1 tcgagttttt ccttgtggaa ttgtcctgtc gtggtttctt cctcgttctt ttttagaggc
       61 tcccattgct ggaacaagcg gaggaaagag cgttctttct caagttgctc caatttatct
      121 aggacttttt gaagatgaga aaattcatta agaattttct cagactcttg gatcttatct
      181 cgacacttgg ctatttgctg ggtgatctcg tttccgttag ccacattttc cagaagaatc
      241 tctaaattct cattgggctc ttcaatcagc atattttccc ttacaaagtg atcaatattt
      301 tcaatggact tcacataaaa agcccgattg aaaattttaa gatcttcttt agtaattccc
      361 cctagaagag tggatagtgt agagaagtaa gattgagcgg tatcgaaaat ttgagaagac
      421 gggtacttct ccattaaagt ttctttaaat ttttttccat ttttcggaag attcatggcc
      481 gcgccacaat aatccttaac tcgcatatct ttttgcgtga tatacaaaag acgatcttga
      541 atttgataat tgctgtacca ccaaataagt ccggcagaga tataggaggc tggcgaatga
      601 ttattttgca aacaaagttg caagacggag cagccggact ctcgattata aacattagaa
      661 agatttttat tctccttccc tgttgttgag gcatacttac ctaaaacata atccgtcatg
      721 cttcgcccgc cggccgcacc tttttcataa tcgttcgtga cgcccagttt tagcagatta
      781 tcaaagggca aaagcccaat cataatccca tcgataagag tggattttcc ggacccattg
      841 accccaagga tagtcgaagc cggaggagga gcaaagaggg gccctagatt catttgatcc
      901 cgaaaaacag cctcctgata gcctgtgaag tttccccaat tcaacatttc gacccggata
      961 agagagtgac ctttttcaag cataattagt tttcctccgg agcatcttcg atggaactgg
     1021 attgggaaag ataggcctca ataaaagcgc gagtgcgttg agtttcctca aagggcaata
     1081 aaatattgca aaccggtgaa atttcaaaaa catcctcaga ctccggcaac tcaaaaagta
     1141 cctgcagttc tttgagttcc cttatgctgt ccttcagttc tctttccacc gcgcgatctt
     1201 ctttgtattt ttcaatatga cttaagaaag gaagtatttc atctttgata ctctgacggc
     1261 ttatagcgca tatttcattt tcaggggcat tctgataatc caaacgacgt ttagttaatg
     1321 aaagaagaat catagtcgat atttttttaa gagtgagagt cgtccccagc tgaaagccaa
     1381 ttttttcttc aagctcggcg tcccctgggg ccagataagc cacccccagg ctttcgttga
     1441 tgataaggtg agccccaagc gcttctatgt gagagctgag ttgaacctga cagtccacga
     1501 tatttcgaaa ccattcgcgg gacttttttt gaagtagaaa tctattttta aggaggcggc
     1561 gcaggcaacc cgcgatgatt atattatggt ttgaaatcat aacttctgag ccttatgttt
     1621 aagaaggtag ttgggaactt ttcttaggac gatattgata ttctgtaaag aaagagaaat
     1681 atcaatttcc ttctggaaga tcttttcaat tgccggatct agtgtgtccg cttcgatgag
     1741 ggcataaagt gccgccaagc tgtgaaggtg cgggtaatcg gcaagtgcca cggggccgta
     1801 ttgctgtaaa tgttttttta aatcctcaag aactctttga atggtgtcct cctccgcctt
     1861 aagcaaggcc tgcatcatga agcgaagttc ctctgccgtg agatcctcag aaaccaaaga
     1921 cactgcctct gcctcttttt gaatatggaa ttcattggta tccacagacc caaaatcagt
     1981 ttcgcaagga atttttaatt ctaaattcaa accttcaata atggaaagag cattttcttt
     2041 atgagcatat agaagccgga agatcgcatt gatattctct tggattaagc gcgaatccgt
     2101 agtcaaggca taagctactt gctgttgaag gatcctgatc tgcactcgag ttttctcttt
     2161 gatacttgag tcggcggcat caaagtgacg tgcgaagtcg gcaaggtagg atcttttgag
     2221 cacgtcttca gcaacgatgt ttcgcgattt taataagctc agaattcgtg gtatattgga
     2281 gtgcttgtga cgagcggggc tgccctccaa ttgagagaaa agttccttgg cttgattgta
     2341 gcttttaaat tcttcggtgt tattaagatt caggtggaag tccgcaatcg tgcgcacatt
     2401 ggctccaggt gtttgctttt gctgcagata gttttcggca agaactttcc gctcccgctc
     2461 tacggccgcc tttacgtcct ctgtcgccga gagaatgtgg atcgcagctt cttccgcttg
     2521 actgaaaaga gcttcattgg agtgatgaaa ggggagtaac tccgctgctt tgaggccatt
     2581 ttctttgata cgcttaatgt cagcttgata agctgcgatt ttttctttgt gatgctgaag
     2641 cgcatcttct tcggtcattt cattgcctga gattaaaagt ccattgaggg ctcctaaaag
     2701 agtctcagcc cctgtgccgg aaaaccgtct tctttcggaa agaagagatt ccatcatttt
     2761 tagaaaatcc ttccccatgc gagtggcttc gatatatctt ttaccaatac tgtcgtggga
     2821 gtctttaaga acttgtaact ccacaatcat gatttgaaaa tactctgcaa ctttgacttc
     2881 aacattttga accttgacag aatagtattg aattaaaatt gctttgagaa cgtcgtaagg
     2941 cactctggta ttttcttcct caagaaggtc ataaatatgc gctaggaggc ctatgtattc
     3001 atcccaatct aattttttga aaaccaaaag atagggatga gttgcgaaga ggctctcaag
     3061 agattctcgg tggataattt cagaacgctt agccatgggt atttatataa tggtaaaatt
     3121 catttagata gttgaactat gtccaaagta gttttttctc cgtctttttt cctctaaaga
     3181 tgagctaaaa tacttattca tagtttttat agcagaatca gtgagtactc ttttttctta
     3241 gggagttcac tagggggtat aaatatttgc caatactgct caagaaattg gaaatattct
     3301 tccatcgttt tcatgatttt tgcccccgca gtttttcaat ctctggccac tcttgaaaaa
     3361 gatccgcata ttttttaatt aatgtaaaat ccaagtttgg cattttcaaa agtctttgga
     3421 tatcagcttt atcttgaagc tcacgacttt gatcattttt gtaagcttga atttttaggc
     3481 ctacaatatc ctctggtctt acgacgtaaa ctccaagttc agtatttttg agagcttgct
     3541 tgagcatttc ttggctgaga ggacggttgg caagaacaat atcgacaaac cccatgccag
     3601 aaaattgaag aacttcgggg gactcgtgct tgagtacaaa accttttgct aaaagagagg
     3661 tcttaataag ttctttttta ccgccatcgg cgagaaagtc gatatcgacg gtggctctgt
     3721 gttggccata gacagcaagt gcaaacccac caattaatgc gtgggaaaca ccttgttgct
     3781 ctaagatgga gtgagcctcg gtcaaagttt tacgtagatt aatcacttaa caagggtatc
     3841 aatagcttag tgaagagaaa ataaaaaacc gagattaaag agaaatttaa atggatgccc
     3901 gaccagtgac ttgcgcaagt tttgctatcc tcatgatatt attgaaaatt attgtacacc
     3961 gcaagttcga attattgaaa ttatagcacc tttatacaaa gaaaacttat ccattaccga
     4021 aattgctgaa cgaacaggct ttaaaagaac aatggtttgg gagactctta agcagcacga
     4081 aaaggagtta aagtctcagc gcgttgttcc ttatgagcgc tggcggaagg gacataagaa
     4141 aacgggggta cgaccgccgt atggtttttg ttttcttcaa ggggaggttg tccgtgatcc
     4201 caaagagtac ccaacgcttc ttttaatgca caatttatgg actcgcggta gtggaaatat
     4261 gtctattcta gcagctccct ttggtattga tagcccattt ctagtgcatc tcccactagt
     4321 ctgttcagat ggcgagtgga gagtcgcgct acctcaagag cttgcacaaa atgatttgag
     4381 aaattctgcg gcgtggtttg ataaatgtga caaaactcat gcaaagaagg ctggtgttga
     4441 aagagggact ttatttgaat cattgaacta caacaataaa ttgcttgtac aaaacccctc
     4501 tgcgcgacgt tggatagttt ttaatgctgc ggggacaaat gtctcggctg caacggttga
     4561 aaatacagaa ctgttttggg ctgatcaaag aacctattgg tattgtccat cttcaaaggc
     4621 tgaaggagat tttttggcag caattctaaa tgcgccgtca ttaaatttga tcatcaagcc
     4681 atttcaaagt gttgggttgg ctggagagag agatattcat aagaagattc ttgagctttt
     4741 gccaaagtac gatggctcaa attccaccat ggctgagata agtaaactcg gagcagaggc
     4801 aaagacttca gttgatgcga ttctagcgac tctaaagggc aaaggtattg tgacaaaaag
     4861 aaaaacgact agagatcaca tccaaaaaac actaaattta attgataaag cagtgaaaga
     4921 cttaattcag attaaagggt agcgtcgagc ggacgcttcc tattgatttt ctcagctttg
     4981 gaaatctgtt tagcaacgaa tccagttttt gattttcgta cagagcaaac gatttcgtct
     5041 tttggattca gacggatggg tttggcagaa ggtgtaattt ccaaagcaga aagaacgagc
     5101 gtgcggccca tttcatccaa tgcttcacca agtccttttt tgcgatcaaa ccaaattacc
     5161 ttcgctgtta ccatacttaa ttattatcct tatttggcac ggcaatgcaa caaaagagcc
     5221 gtactgacaa ggagtggtgt gttgcacaat tgtccgactt acggactgtc caacattctg
     5281 atatagctgg cgaatctttt gaatgaagtt gggagcaagg gtctattgaa ggtggaaaat
     5341 aataaggcaa attataatat tatttcgttt ggtaaaagat ttgaaaattt agcgatttca
     5401 gtgaagagcc aaattatcgg ttcaaaactg agcacgtttg aggcgaatgt taagtcggga
     5461 tcaaaaatat tcctccactg tggaagccaa atctggggac ttgctacggt gattggaaat
     5521 tatcaatata accaatcaaa agtctggagc aatgaccttt atccgcatcg gtataacatc
     5581 aaggtggatt tactgacgtt gcaaccgctg catctttcca acggcattta taatgtccaa
     5641 ctcagagaac aatttggttc gggctgggca tataaattta ttttctcgcc acgccctctt
     5701 cctggtgacg tgggtgaaaa aattgagaaa gacctcagga cagcaccgac ttgtgatctc
     5761 gaaacctttc ttagcgcaat taagggttag tttgaactgt tgtagtcatt cgcagtagtc
     5821 atcgaaaagt tactacaacc aatgactacg gttttattgc gagagagaat ataccgcagt
     5881 aggaaccagt aggttcactt caagacttga gaaacctcct caagagtaaa tcgctgttat
     5941 tattgttgtt ctctccaaaa tcaaaaaccc gcttgtggcg ggtttgaaat tggatggggc
     6001 ggctggactc gaaccagcga atggcagaat caaaatctgc taacttacca acttgtcgac
     6061 gccccaacat gaattgaatt atgagcttcg taaattaaaa aaaagcggtc ccgagagcaa
     6121 gtaaattctc ggaagcgctt aaatttaccg caggttaatg caacgcaata ctagaaggta
     6181 ggccgtacct ctctcctagg gttcgtcgtc gtaggaggtg gtgggggctt tttggcgctt
     6241 taagtttact agggtggcgc ccatggactt tagttctttt tcgtaggcct caaagattga
     6301 gtcctcaatc attgcccggg tttcttgatt gaggggatgg gcaatgtcgc gaaattgacc
     6361 gtctttgcgt ttcttgcttg gcatcgcaat aaagagaccg ctaggcccct gaataatctt
     6421 aagatctctt accacaaagc tgccatctat ggtgattgaa acataagctt tcagtctttc
     6481 ctcgtttaca ggaaagacct taacttcggt gattttcatg cgtccgtcct ttgctgttac
     6541 gtgcttaatt aggggactat ggttgttctt ctctctctgt tcttccatat aaaccaacgg
     6601 caattttgag ttaaagataa agaccttcgt ccaaattagc tgcgtccaat cactgtttta
     6661 tgagaaaaat gtctcatttt ggtatgtggc caaaaaacat aaaaaaagcc cacgtatcaa
     6721 cgtgagcttt ctaaataaac tatttttgat gataaattaa atcttatttt gtgtcaatag
     6781 tcacaatcat cagacgattt ccacgagcaa gtcttagagt gttcgttcct ttttttaaac
     6841 tctttgaaac atcgagagcc tttgtaactt cttttttgtt tatatctaag atcaaatctc
     6901 cgacacggag gtcagccatg gctgcaagac tgtcgcgttc aattcggata atcatgggat
     6961 tcataacctc ggaagggatt tgaaactccg cccgtaattc gtcattcaga tcgctcacgg
     7021 tgaagcccaa gttaaagggg actttttgtc cggagtaact ttttgaagag ccggggtttt
     7081 tattcagggc cgcgaagttt ggttttgaga ggacggtcat tttaatactg acaggcttgc
     7141 gttcgcgaat cgctttgagg gtgacggagc ttccgatttt agaatcttcc acggcatctt
     7201 tgaggtccat agaacttttg attttcttgc cgttgaattc agtcacaata tcataagggc
     7261 gaagtccggc tttgtcggca ggtcctttct tctcaagggc ggtgatgaca gcgccttcgg
     7321 gttcttgcag gcctaagtaa gtagggtcaa tgctgggatc cagatcgcca agccttgcgc
     7381 caatataacc tttttcaatg cgtccgcttt tttccaactg cggaataatg gctttgactt
     7441 catcaatggg gattgcaaat cctattcctt gtgctctcgc atcgatcgcc gagttcacgc
     7501 caataacata acctttggtg ttcacgagag ggccaccgga gtttccagga ttgatactag
     7561 catctgtttg aagtagaggg atcttgttga tctcaccgat ctcacggccc ttggaagaga
     7621 taatcccttt agtcatggag tgtccgtggc cgtatggatt accgaaggcc gccacccatt
     7681 ctccgacctc caagtctttg cttgaaccca gggctgccac tggcaacttg ccatccggcg
     7741 tgattttgat caaagcaatg tctgagcgct catcatagcc aatgactttt gcatcgtaga
     7801 gcttgtctga tttttctgtg agttgaactt gaatgccatc cgctccttga atcacgtgat
     7861 tgttagttac gatcagaccg tcttcacgga taatgaatcc tgtgcccaaa ccgtagggac
     7921 ggggcttgcc ttgaggagcc atttgctgtc ggcgagattg caaaggcacg ccataaaact
     7981 gctcaagcat ttcgagcatg gggtcgcgtt gcaggttatt cattttcggt atggcaagag
     8041 tggagatatt cacaaccgca ggattgatga ctcggccaag ctccacaaac agatttcccg
     8101 gaaggggctc tgaaagcttc atggctggag gcgttttttg tggaatttgg gcgtacgtta
     8161 gatttaatcc tgtgaccagg ctgagactta taagagcgac aaatagtttt ttcatagatc
     8221 ttcctcctac gattgaacga atttaatttg caaatcgctg attaaatttt ctaagaattt
     8281 tttttcttct gcagttaggt tatttttagt tttttcttgc aacatcacga gcaagtcgat
     8341 gttgaagcgg gccatgtttt tatctttttt caaggcgcct cccggagagt ctggagcaag
     8401 acccatggcc atcactgcgg aagaagcgat ggacatggcg aggactgaaa aggacgcttc
     8461 catttgttcg ggcataaaca cctcttagat agggtagtaa cccatcagtt tctgcaatcg
     8521 aatttcaatc gatggatggg atttaagtaa taatttttga cggtaacctt cggggttcac
     8581 aataaacaaa tgatgggagc acggaggagg acgcagaggc tgcgtttgcg ccaggccttc
     8641 gagacgccac agcacttcag ccaagcggaa acggtcatgg ataagctccg cagcttgtag
     8701 atccgtttca taatatttct tatgactatt ggcgagctta actaagttcc agcctaaaaa
     8761 agaactcatc ttcaggaaag gagtgtgtcg ttgatgagct aacaaaaaat agttgggcgg
     8821 ccagatgtga tcaagtacgt tgccgagacc cactaaagca tttgccaggg cgctggtcac
     8881 tcccaccata aatgaactca tggtgttaag gttgcaaagt tgatacgcca tcacggcttc
     8941 aagatcttct ttgctgaatt tttgcaccaa tcctgaagac agcgcgacag atcctcttct
     9001 ccataaatga tttacacaaa atgcggtttc tgtttccagg ggaatcaagt aaagatctgg
     9061 cgggtttatc cccaggtgat cggaatattt ctgtaagatt tggtcaagac cccaaggatc
     9121 ttgtccctgc atttgtttag agtttaaagt ggtgagaaga gaagactccc catagaagaa
     9181 aacgaaaaaa ttaagggcga cgctagacag aaaacccatg aagagtccca aacgctcccc
     9241 gatttcataa ccgagaataa ggaatgccag agaacttagt aaaatgaaga cccagacttt
     9301 agtagagtta gcaaccatac tcttattttt cccatgcgac gatgtgcttg gcaacagaaa
     9361 gtagtgcata gcagcagtaa gactaagctt gcgaagattc cagcaaaatt gtgaaataaa
     9421 ccagagtttc atcggaggtg ttttcgtgag cgaaattgat gtcctggccc ttaatgctgc
     9481 tattaaacaa gaaagccaat ttgtagacaa aatgatgcaa gaaatcagta aagtcgttgt
     9541 cggccagaaa gaaatggttg aaggcattat gatgggtcta cttacaggcg gacatatctt
     9601 gctcgaaggg gttcccggtc ttgccaaaac gctcaccatt gccaccgttt ctaaaaccat
     9661 ctccttggat tttcagcgcg ttcagttcac ccccgatctt ctaccgactg atttaatcgg
     9721 aactatgatt tttaatccga aaacaggaga gtttaatccc cgcaagggac ctgtcttcac
     9781 caatatcgtc ttagcggatg aaatcaatcg ggccccggca aaagtgcaaa gtgccttgct
     9841 ggaagctatg gccgagaagc aagtgaccat cggtgatcaa tcctataaat tagaaaatcc
     9901 tttcttggtt ctggcgacgc aaaatccttt agagcaagaa ggcacttacc cattgccgga
     9961 agcccagatg gatcgtttca tgtttaaaat tgacgtggtt tatcccacca agggtgatga
    10021 actggaaatt ttaaatcgta tgggcatgaa cggaaaacca agcgtgaatc cggtgatatc
    10081 aaaagaagat ttgctgcgtg tgtctgaacg tgcggatcag atttatgtcg ataataaaat
    10141 caaaaactac attgttgaaa tcgttatggc ctctcggcat ccagcggagt atggtttaac
    10201 tcgcattgtg aatttgatca gcgttggggg ctctccccgg gcgaccatcg gactttttag
    10261 agcggccaaa gcccacgcct ttattcgtgg tcgtggatat gtgactacag aggacgtcaa
    10321 agcggtggct tataaagttc ttcgtcaccg tatgatcctc acttatgaag cggaagcgga
    10381 aaacattaag acagatgata tgatcaagga aatcctgacc caggttgagg tggcctagtt
    10441 tgagtcttcc tcctgaggtc cttaaaaaag ttaaaatgct ggagatcaac accaggaaac
    10501 tggtgaacaa tctctttgcg ggagaatacc atacccactt caagggacat ggaatgactt
    10561 tttcggattt ccgcgagtac gttccaggag atgatgttcg agccatctct tggccattga
    10621 cggctcgcac cggtaaaacc tatatcaaaa ctttcgaaga agagcgggaa ctcaccatca
    10681 ttttagcggt ggacattagt ggttcgacgg attttggtac cggcgaatac tttaagggtg
    10741 aggtgatgac tcatctggcg gctcttatcg ctttttccgc agttaaaaat aatgatcagg
    10801 tgggcttgct gctattcacg gatcaagtgg agcatttcgt gccacctaaa aaaggtcgag
    10861 gacacattca ccgcattttg cgggatcttt tgtattataa acctaaaagc cacgagacca
    10921 agatttccac cgctttaacc tacctccagg gatttttaaa aaaacgagcg accattttta
    10981 tgttctcgga tttttttgat gagaattatg atcaggcttt gcggatgttg ggacgaaaac
    11041 atgaagttgt ggcctgcgtg gtgaacgata aggccgaagg ccaacttccg gatatgggaa
    11101 ttgtggaatt acaagatcct gaaaccggcg aggttctcac tgtggatact tcttcagcgg
    11161 tctttaaacg ggattattta cgggaattta aaaagcgcaa ggacctgcgc gataaacaac
    11221 tgcgattagc gcaagtggaa agaattgacg tgaattccaa tgaggactat gtcgatcctc
    11281 tcgtggcttt tttcaagaag agatagcgga atatatggca gctgttcagt gtaatttgga
    11341 cattccaaag gttccaggtc ttgaggatca tcaactcacg gtgggtcgtg aattttattt
    11401 aacctgcaag ggggagtggc ccaaaacact gaaaaaggaa gccttatact tcaccttgga
    11461 gaaggctgga agcgaagtta aaagtgaaac tctaaaatac cacatcaagc ttctggactt
    11521 tgaattccgt tctctagaaa acgtggatat caaaatcaca agctatttag ctggccagca
    11581 ccagtttccg aacttgatat taaccgatgg tgagcggaaa ttggaattgg gtccggtgca
    11641 attttcggtg cagagtgtac tggaacaagg acagcaacag aagcaagaaa aagtagagcc
    11701 ctttgggcct atgggacctg ccactattgc agtgcctgtt ttgtactggg tgattttatt
    11761 cggcaccctg gtactcatgg gcactttagg ggcgctcaag atttggcgtc ataatcagcg
    11821 tcgcgaaatg ttgttacgtt tgaaggagca cgattccgca ttaagccctt tgcaggagtt
    11881 tcatcaaagc atgagacgct tacaaagagc caaccccgtg ttttttggta aagaagccac
    11941 ctccgaagaa cttcgccagg gagttacaga actttctcgc atgtttaaag tttttatcag
    12001 tcgtcggttg aaagtgccgg cttttgaatg gaatgaacgg ctgatcgtga acgatcttcg
    12061 tcgttaccat ttctttgtcc atcaggaata ctctcaaaaa atccgcagtc ttttcttgga
    12121 actccatcgg gcggaaagtg cgaacgccaa attgacttct catgatgtga gtcagttggc
    12181 ggaaagcctg cgtaaaactc ttgagggtat tgaaaagctg atggatcgcg agcaaatggc
    12241 tcagaagggg gaccgatgac ttcgtggcat agtccctggg ccttggtatt gctgattcct
    12301 ttgctggtca ttttgatatg gaattatttt cagcgcaaac gaaagatccc ttctctgcaa
    12361 tttagttcac ttaaattaat taagacaatt gcgccgagtg tgcggacgcg tttgatgatt
    12421 gttccctcgt tgatgaaagc cctggccatc gttttggcga ttttggcttt ggctcgccct
    12481 caggaagcca ataccaaagt gaaaaaaaat gtggaaggaa ttgatattgt tatttgcctc
    12541 gacgtttctg atagcatgtt gatcgaggat atgaagccga caaaccgtat cgaggccgct
    12601 aaaaatacca tttatcagtt cattgaaaaa agaacttccg atcgtatcgg gttggtggtc
    12661 tttgccggag agtcttttac actcgtgccg ctgactttgg attaccagtt gattctcgag
    12721 cgggtgaaag aaatcaccac tgccgcgaat gcgaatatca aagacggaac ggcgatcggg
    12781 gtggctttgg ccaacgccgc tggccgtttg aaagattcca ctgcaaaaag ccgcattatg
    12841 attttcatga ccgatgggga aaacaacagt gggactattg atccagagac cgctttggag
    12901 atcgccaagg gttatgaact taaaatttat accattggta ttggtaaaga cggtccgact
    12961 aaaatcccca tttatcagcg cgatatcttt ggcaataaag taaaaaccta tcaacctttt
    13021 gatagtacgg tgaatgatga acttttaagt cgtatggcca aggataccgg cggccagtac
    13081 ttccgtgctt caaaagaaga ttctttagag ggtgtcttta aggagattga ttctttggag
    13141 aaaaccaaag tggatgtgaa taaatacact cgttataccg aattgtttcc gccctatttg
    13201 caggcggctt tggtccttta tcttatcggg gttcttctgg cgaaaacctt cttaaggagg
    13261 gtgccataat gcgattcgaa aatcctttgg ccttttactt cctttggacg attcctttgc
    13321 tgtggatttt ggcggtattt tttcaaaagc acgtggctaa gaaaatggcc aaaaccattg
    13381 ggccaagact ctatcctttc ctgacaagtt cggtgtccag ctataagaga aatacgaaac
    13441 gcgttttaca aagcctggtg attttatttt tcgttatcgc cttggctcga cctcaattgg
    13501 gtcaaagtat gcaagagatt aaaagcgaag gggttgagat catgtttgtg gtcgatgtct
    13561 ctgaaagcat gttggcggaa gatgtgcgcc ccagtcggct agagcaggca aagacagatc
    13621 tcagccgact gctggaccgc atgccaggaa atcgtgtggg agtgatcgct tttgctggat
    13681 cagctggggt attgtctccg ttgtctaatg acccgaatgc gaccaagatg tacttggagt
    13741 ccttgagtcc aagttcagtc agtagtcagg gaaccagttt tgagcaagcc ttaaaattgg
    13801 cggcggatgc tttcaagcgg ggcggagtgg aggaggaaga caccgtgaag gtcacccggg
    13861 ttattctgat tgcttctgat ggagaagatc atgagcctgg tgctatcgaa gctgcgaaaa
    13921 aattggctca gcaaggggta aagattttca ccttggctta tgggacggaa aaaggcggtc
    13981 cgattccaat gaaggatggg ttgggatttt ggaaatccaa taagaaagac cgctcaggtc
    14041 aaaccatcat tactagtgtg aaaggggatg agctgcgagc tttagcccaa gccggtgaag
    14101 gttcttttta ctttgccact tacggaagtg attatattaa aaatgtggga gaagatattt
    14161 ccaaactaga gaaggtcgag tttgaatcca agatggctat tcaatatgaa gagcgcttcc
    14221 aattattttt agtgattggg attctattag cgttgattga actgttttta ggggagcgtc
    14281 gggcgtcttt ccgtctgtgg aaaggacgct atgaggtacc accggcatga gtagaagttg
    14341 gggcgtgctc ttaatcagtg ggatgttggc tttatctgca atcttcttaa gttggagtgg
    14401 cctgattgaa tggcctactt tgccgcattt aaagagtttg gtgctgaacc gtcaggggaa
    14461 tcagcaactg aaagagcaga attactcttc cgcatttgat gcctatgtga aaacgcttga
    14521 atttgagccc tttgctccgg agatccattt gaacttgggt cttacctatg aaggacagca
    14581 gcagcctgaa aaagcgatgc catcgtacga gaatgcttta aagtacgcgc aaagtaatga
    14641 aatgaaattc gcgagccttt ttaatcaggc acagctttta ggtaaggata aaaaagtgga
    14701 tgaagctctg gagttttatc aaaaagcttt gagcttggat cctacttcga aagaagttaa
    14761 aaccaatatc gaacttctga tccaaatgca gcagcagcaa aaaggtgggc agcaggataa
    14821 taagaacaaa aaagatcaaa aagacgataa ggatcaaaaa gataataaag accaaaataa
    14881 cagtcagaac aataaagatc agaaggactc taaggatcaa aaagacaaca aagatcaagg
    14941 caaagacgaa aaagacgaca agagcaaaga tcaaaaggat caaaaagaaa agtcccaata
    15001 cagtcagagc gaaaaataca aacctcggcc ctttaaaggc caagaactct ccgaaggaga
    15061 tgtgaaaaag atcttgggcg agatcaagca gcaagagcaa aaaattcgtt ctgagtacaa
    15121 tcgaaaagaa gtgaaggagc agcctcgtga caaggattgg taacaggttc agtctggtgc
    15181 ttttgttggt gagtttggca gttcctgctt gggccaaggt gagtgttcaa gcggtggtgg
    15241 atcgcaatga aatgagtgtg ggtgattcct tcactttgac agtgtcagtg aactcagaag
    15301 aatccgtgga tattaaagag cctcgggctc cggatatgga aggattcgaa cttttgaata
    15361 gctggcaatc cagcagcacg tcccaacgtt tggtgaatgg ctccggcgga atgcattttg
    15421 aagtgcaacg ccgacaggat tttaattatc tcttggctcc acgaaaaaaa ggcacctaca
    15481 gcgtgggagc tttcgaagtg gttgtcgacg gaaaggttta tcatagccag ccgatgacca
    15541 tcactgtcgg cgaaaccggc agcgcgggcc ctcgcaaggg aaggggaggc caactcccgg
    15601 gaagacggct cattccgccg ggatttggca atatgccagg ggctgatgtg ttcgaagaaa
    15661 tggaccgcgc ggaagaagag ctgtttaatc aactcttgca acgtcgccaa caaggcggac
    15721 tgcccaacgg tggaatccca ggggctcctg gaggaagatt tcaagcggaa cctcaatatc
    15781 gttctttgcc gacaaatccc aatgaagctt tttttatcca agtcgaagtg gataaaacgg
    15841 atgtttacga aggtgagcaa atcaccgcga gctggtattt gtacactcga ggacaaatgg
    15901 aaagtttgga tcgcct
//
LOCUS       DATBTE010000200         6617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_709499, whole genome shotgun sequence.
ACCESSION   DATBTE010000200 DATBTE010000000
VERSION     DATBTE010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6617
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709499"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VIG33_07415"
     CDS             <1..489
                     /locus_tag="VIG33_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8270702.1"
                     /translation="DWFPIGSLKADNYDELTLWGQIADRVHHFVLPLICYMIGGFTQL
                     SYLMRNSMLEVVKSDYVRTARAKGVSERVVLYKHALRNALIALVPGIASFFGVFLTGS
                     LIIERMFNLDGMGLLSYQSLIVRDYNVIMGITFISSLILVIGRVFSDIVYVLIDPRID
                     FK"
     gene            486..1523
                     /locus_tag="VIG33_07420"
     CDS             486..1523
                     /locus_tag="VIG33_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8270703.1"
                     /translation="MMDPVEKYLISNELTLKRYRRFKRNRSAVISVFIFLFMIFTSFT
                     AEFWFNSKPHILKYQGELYFPIVKDYHPTLFDRTDIQVMDYRSLKMGSEDWAIWPLAQ
                     WNPYESNSFVESYPAPPTKYNLMGTDDRGRDVFARLLYGFRYTFVYAVGVWFLTYLIG
                     CAVGAGMGYVGGKVDLIGMRAIEIFESIPSFLILITIISIFKPNILLLIVFSVFFNWT
                     GISHYMRGQFLSLRKREYVEAAKAIGSTHNRVIFKHIMPNALTPIITLSPFEIAGNIY
                     TLSIMDYLGLGLPPPTPSWGELLSQGQSYFTTAEWLVWYPTGALVLTMSLLININLAV
                     RDAFDAKSAVG"
     gene            complement(1820..2167)
                     /locus_tag="VIG33_07425"
     CDS             complement(1820..2167)
                     /locus_tag="VIG33_07425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEY8270704.1"
                     /translation="MTMTTYKPFDVIELPFPFSDLSSSKKRKALVLSSEEFNVKNKAT
                     TVLMITSRANSEWAGDIQLDDWNFAGLKKPCYARLKIFTADNDLILNKVGSLSQNDIK
                     KAIQSFRKYLPVF"
     gene            complement(2148..2381)
                     /locus_tag="VIG33_07430"
     CDS             complement(2148..2381)
                     /locus_tag="VIG33_07430"
                     /inference="COORDINATES: protein motif:HMM:NF027267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY8270705.1"
                     /translation="MGALKTSTISKKGQTTIPLEVRESLNLKEGDTVVFIIGNDNEIT
                     LKKAPSLEEDLAYLKLIEKTLAPEWMSDDDDDL"
     gene            2585..3850
                     /locus_tag="VIG33_07435"
     CDS             2585..3850
                     /locus_tag="VIG33_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY8270706.1"
                     /translation="MGPQDSLDFKNIFESVPGAVLVLLPDDPKFTIVAANHAFEKLTK
                     TNREKIIGRGVFEVFPDDPSEPSPTGMKRTRASYRRVMQNRAPDTLAVMKYNIQNPEE
                     DGHGFEEHYWSVANSPVFNSDGSLGYIIAQPEDVTEFMYLKLRGLEQTQLMDELQSET
                     ARMQSEIFIASKLYADSQRATQEREYILSIVANDLNSSVSAIKLSTQYLTKKDIQTEE
                     AHSVARNILQSTQIIERLISDMLDFGKIQSGTLLLKKDSESVGEMVKAAMESVMPMAL
                     EKRIQLHTDLPNTDMRISCDKNRIIQVLWNLLVNAIRLTAEKGEVKIKACKLGDNVQF
                     SVTDTGPGVAAEEISKIFDRFWQAKELGNFATGLGLAIVKGIIEAHGGEIWLESQLSQ
                     GSRFYFTLKAIDHTWRKTQPIPQEPESIL"
     gene            complement(3885..5792)
                     /gene="speA"
                     /locus_tag="VIG33_07440"
     CDS             complement(3885..5792)
                     /gene="speA"
                     /locus_tag="VIG33_07440"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEY8270707.1"
                     /translation="MSNNWSSEKSASLYGINNWGNGYFRVNSNGNVSVTPMGATGPSV
                     DVYELTQDLLDRGIRVPIMIRFPDIIKARVQLLNSCFQKAFVDHSYKGQYCGVYPIKV
                     NQQRHLVQELVKHGRDFGLGLECGSKPELLVVLALMNTQNALIICNGFKDAEYIETAI
                     LSQKLGRNTIIVVDRKEELKMIVDAAKKFNSRPKIGFRAKLNTQGAGKWVDSSGARSK
                     FGLTSMEIVEGVELLKKENMLDCLELLHFHIGSQVPSIQSIKSSLKEGARFYTELHAM
                     GAGLKYIDVGGGLGVDYDGTGSSDSSINYSEQEYANDIVSVLQSLCDEKNIPHPNIVT
                     ESGRFLVAHHSVLIFNVLGVNDLHRIDPPPPANKKDPTIMQDMQYIFEKVNRDNINEC
                     FNDLSQVKQETLQLFTYGVLTLAQRAWCESMYFTIATKMTILAKATPDTEDIQAALSK
                     ELCDTYFANFSVFQSVPDSWAVDQLFPIMPIHRLGEEPTRSATLADLTCDSDGVIDKF
                     IDTESGKPKETVHIHQYTEGQPYYLGIFLTGAYQEILGDLHNLFGDTDAVHISLSEVG
                     YTIDHYVPGDTVTEVLSYVQYGRSEMVDNVRQATEESIQRGTITKQEAKLLIKHYEEG
                     LSGYTYLEEAE"
     gene            complement(5811..>6617)
                     /locus_tag="VIG33_07445"
     CDS             complement(5811..>6617)
                     /locus_tag="VIG33_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY8270708.1"
                     /translation="LYARFWMKVLFDAGLVTHDEPFKKLAHQGMILGEDHQKMSKSRN
                     NGIAADEVSQKYGADALRTFVCFMGPLESEKPWSATGIEGVKRFLDRVTRLILNEENQ
                     CIATNPGIEPISTELEKLLHKTIKKVTEDIEKMSFNTAISAMMILVNELYKANVKPKS
                     ILKPLIQLLMPFAPYMAEELWEKMGGEGFVSLAPWPKYDSNLIADDTVTIGVQVNGKM
                     RGTIETAMDAPEEVALKTAYAVDTVMAALNGKTPDKIIYKAGKILNLIIK"
BASE COUNT         1782 a         1323 c         1491 g         2021 t
ORIGIN      
        1 gattggttcc cgattggaag tttaaaagcc gataattatg acgaattaac actctgggga
       61 cagattgcag atcgcgttca tcactttgtt ttgcctttga tttgctacat gatcggtggc
      121 ttcactcagc tctcttattt gatgcgaaac tcaatgctcg aagttgtgaa atctgattat
      181 gttcgcacag ctcgagctaa aggtgtttcc gaaagagtgg ttctttataa gcatgcgctt
      241 aggaatgctt tgattgcgtt ggttccaggg attgctagct tctttggagt tttcctcact
      301 ggatctctga ttatagaaag aatgttcaat ttggacggca tgggacttct cagttatcag
      361 tctttgatcg ttcgtgacta caatgtgatt atgggtatta cttttatctc ttcgctcatt
      421 ctcgttattg ggcgggtatt cagtgatatc gtctatgttc ttatcgaccc aaggattgac
      481 ttcaaatgat ggatccagtt gagaagtacc ttattagcaa tgagctgact ctgaaacgtt
      541 atcggcgttt taaacgcaat agatctgcgg ttatttcagt ttttattttc ttattcatga
      601 tttttacaag ttttacggcg gagttctggt ttaacagtaa gccgcatatt ctaaaatatc
      661 agggtgaatt gtattttccc attgtgaagg attaccatcc gactcttttt gaccgcactg
      721 atattcaagt tatggattac cgctctctaa aaatgggatc tgaggactgg gcgatttggc
      781 cgctagctca atggaatcct tatgagagta attcttttgt tgagtcttat ccagcaccac
      841 caacaaagta taatcttatg ggtacagacg atcgcggtcg tgatgtcttt gctcgcttac
      901 tctatggttt ccgctacact tttgtttatg cggtgggagt atggtttctg acttatctta
      961 tcggttgcgc ggtgggcgcg gggatgggtt atgtcggtgg aaaagtcgac ttgatcggaa
     1021 tgcgtgcgat cgagattttt gagagtattc cttcgttctt gattttaatt acaattattt
     1081 ccatttttaa gccgaatatt cttttgttga ttgttttcag tgtgttcttc aattggaccg
     1141 gaatttcgca ttatatgcgc ggccaatttt tgagtttaag aaagcgcgaa tatgtggagg
     1201 cggcgaaagc catcggttca actcataatc gggtgatctt caaacatatt atgccgaatg
     1261 cattgactcc cattattact ctgagtcctt ttgagattgc gggaaatatc tatactcttt
     1321 ccattatgga ttatctgggg ctgggccttc cgccgccaac gccaagctgg ggtgaattgc
     1381 tgagccaagg acagagctat tttacaacgg ctgaatggct ggtctggtat ccgacgggag
     1441 ctttggtttt gaccatgagc ttgctgatca atatcaactt agcggttcgc gacgccttcg
     1501 atgcgaagtc cgcggtcggg tagagagttc ttagaataag ggtaggttgt gtcccacgat
     1561 aatgggattt ttaaacaatc gcagtttgcc tggcatggat gaaattaacc cgtgtaagtg
     1621 caagtagcat tttatgcgat tgagttgcat ataatgctac ttggaatata cgaatcgacg
     1681 gggatttatc tttctaattc ttctgtcaca gctctcttta tcaagagtgt tgctttagct
     1741 tatagtcaag acggtgtttt tttcgtctag cacatgaatc ataaagccat agagaccagg
     1801 gatgcaccgc tgtcttttct taaaaaactg gtaaatattt gcgaaaagat tggattgctt
     1861 tttttatgtc attctgactt aaagatccta ctttgtttaa aattaaatca ttatccgctg
     1921 tgaatatttt tagacgagca taacaaggtt ttttaaggcc agcaaagttc cagtcgtcca
     1981 gttggatatc acctgcccat tctgaattag ctcttgaggt gatcatcaag actgtggttg
     2041 ctttattttt tacattaaat tcttcggaac tcagtacaag ggctttacgt tttttagagc
     2101 ttgataaatc agaaaaagga aatggtaatt ctataacgtc aaaaggctta taagtcgtca
     2161 tcgtcatcgc tcatccattc tggagctaag gttttttcga ttaattttaa ataagctaaa
     2221 tcttcttcca aagacggagc tttttttaag gtaatctcgt tatcattacc gattataaaa
     2281 acaacagtat cgccttcttt gaggtttaaa ctttccctga cttctagggg aatagttgtt
     2341 tgtccttttt ttgaaatagt ggatgtttta agcgctccca taagatctcc tcatacttta
     2401 taatatcgca agaaggtatg aatgtaaaga tgtatggaaa tacgacctta ggatggagcc
     2461 tattggatta ggaatataaa aaacgaacca atccaggcat cgtgatttct atatatcggg
     2521 ctggacggcg agacatatcg gcgtttcagg tgtagtttcg tagcaataac aggttggctt
     2581 agctatggga ccgcaagatt cacttgattt caaaaatatc ttcgagtctg tgccaggagc
     2641 agttctggtt cttcttccgg acgatcctaa atttacaata gttgcggcaa atcatgcctt
     2701 tgagaaactg acaaaaacga accgagagaa aattattggt cgtggcgtat tcgaagtttt
     2761 tccggatgac ccgagtgaac caagtccgac agggatgaag aggactcgtg catcctaccg
     2821 acgggttatg caaaataggg ctcctgatac cttagcggtt atgaaataca atatacaaaa
     2881 tccagaggaa gatggtcacg gttttgaaga acattattgg agtgtagcaa attcccccgt
     2941 ctttaattcc gatggatcgc ttggttacat tattgctcag cccgaagacg tcacagaatt
     3001 catgtacttg aaattgcgtg gattagagca aactcagctg atggacgaat tgcagtcgga
     3061 aacggcccga atgcagtcag agatatttat tgcttcaaaa ctttacgcgg actctcaaag
     3121 agccactcaa gagcgggaat atatcttatc tattgttgct aatgatctca acagttcagt
     3181 ttctgctatc aaattgtcga cacagtatct aaccaaaaag gatatacaaa ctgaggaagc
     3241 tcactctgtt gcgcgaaaca ttttacaatc cacacaaatc attgaaagac tcatttccga
     3301 catgcttgat ttcgggaaaa ttcagtctgg aacactgctg ctgaaaaaag attcggaaag
     3361 tgtgggggaa atggttaagg cagcaatgga atcagtgatg ccaatggctc tggaaaaaag
     3421 aattcaactt cacacggatt taccgaatac ggatatgcgg atttcctgcg ataaaaatcg
     3481 aattattcag gttctatgga atctgctggt aaatgcgata aggcttactg cggaaaaagg
     3541 agaagtgaag atcaaagcat gcaaactcgg agataatgtc cagttttcgg ttacagatac
     3601 gggtccggga gttgctgctg aggaaatttc aaagatattt gatcgatttt ggcaggcaaa
     3661 agagttggga aatttcgcga caggcttggg attggcaatt gtgaaaggga ttatcgaagc
     3721 tcacggcggc gaaatatggc tcgagagtca gttaagtcaa ggaagtcggt tttattttac
     3781 ccttaaggcg attgatcata cgtggagaaa aactcagcct atacctcaag agccagaatc
     3841 cattctttag agaaatggca tttggctcac gacgaagact gattttactc tgcttcttca
     3901 agataagtat aacctgataa accttcttcg tagtgcttga tcagtagctt tgcttcttgt
     3961 ttggtgatcg tgcctctttg aatactttct tctgtggcct ggcgaacatt gtccaccatc
     4021 tcggaacgtc catactgaac gtagcttaat acttccgtta cagtgtcacc tgggacgtag
     4081 tggtcgatag tatagcccac ctcactgaga gaaatatgaa ccgcatcggt gtcgccgaag
     4141 aggttgtgca aatcccctaa aatttcttga taagcaccag taaggaaaat acccaggtag
     4201 tagggttggc cttcggtgta ttgatgaata tgcacggttt cttttggctt gccagattct
     4261 gtgtcgataa atttatcaat cacaccgtca gaatcacagg ttaaatccgc aagggttgcg
     4321 ctgcgagtgg gttcttcacc cagtctgtgg ataggcatga tcgggaaaag ctggtccacg
     4381 gcccaagagt caggaactga ttggaagact gaaaagtttg cgaaataagt atcgcaaagt
     4441 tctttggaaa gcgctgcttg aatatcttcc gtgtcaggag tggctttagc caagatcgtc
     4501 attttggtag caatcgtaaa atacatggat tcacaccagg cccgttgggc taaggttaaa
     4561 acgccataag tgaacagttg gagagtctct tgttttactt ggctcaagtc attgaagcat
     4621 tcgttaatat tgtctcggtt caccttttca aaaatatact gcatatcctg catgatggtg
     4681 gggtcttttt tgttcgcagg gggtggaggg tctatgcggt gaagatcatt gactcccagt
     4741 acattgaaaa tcaaaaccga atgatgggcc acgaggaatc ggccagactc agtgacgatg
     4801 ttcggatggg gaatattttt ttcatcgcaa agagattgga gcacagagac gatatcattg
     4861 gcgtactctt gctcactgta gtttatagag ctgtcagagg aacccgtgcc atcgtaatcc
     4921 actccaagac caccgcccac atcaatgtat ttaaggcctg cgcccatggc atgaagttca
     4981 gtgtaaaaac gcgctccttc ttttaaggaa gacttaatac tttgaataga aggaacttgg
     5041 gacccaatgt ggaaatgaag aagctcgagg caatcaagca tgttttcttt tttaagaagc
     5101 tcaacacctt caacaatttc catggatgtc aggccgaatt ttgaacgtgc ccctgaagag
     5161 tccacccact tgcccgcacc ttgagtgttt aattttgcac ggaagccgat tttaggacga
     5221 gagttgaatt tcttcgccgc gtccacgatc attttgagtt cttctttacg gtccacaacg
     5281 atgatggtat tgcggcccag tttttgagaa aggatggcgg tttcaatgta ctccgcatct
     5341 ttaaagccgt tgcagatgat caaggcattt tgcgtattca ttaaagctag gaccactaga
     5401 agttctggct tagagccgca ctcaagtccc aaaccaaaat ctcttccgtg ttttacaagt
     5461 tcttgtacca agtggcgttg ttgattcacc ttaatgggat aaacaccaca gtattggcct
     5521 ttgtaactat gatcaacaaa agctttttga aaacagctgt tcaggagctg gacacgagcc
     5581 ttaataatat cagggaaacg aatcatgatg ggaacgcgga tcccacgatc caaaagatct
     5641 tgagttaatt cgtagacatc aacagaaggg cccgtcgcac ccattggagt cactgaaaca
     5701 ttgccatttg agtttactcg aaaatagcca ttgccccagt tgttaatacc atagagtgag
     5761 gcactttttt cagaactcca attatttgac atcgaaacga ttccttttaa ttacttaatt
     5821 atgaggttca aaatcttacc ggctttataa ataatcttat caggggtttt gccgttcaaa
     5881 gccgccatga ccgtatccac cgcataggcc gttttcagtg ccacttcttc gggagcgtcc
     5941 attgccgtct cgatcgtgcc acgcattttt ccattcacct gcaccccaat cgttactgta
     6001 tcatcggcga tgagattgct atcatattta ggccatggag ccaaagaaac aaatccttct
     6061 ccccccattt tttcccatag ttcctctgcc atgtaaggcg caaaaggcat caagagttgg
     6121 atgagaggct ttaggatact ctttggtttg acgttcgctt tatagagttc attgactaag
     6181 atcatcatcg cgctaatggc tgtattaaag ctcattttct ctatgtcttc agtcactttt
     6241 ttgatggtct tgtgcaatag cttttcaagt tcagtggaaa tgggttcgat tcctggatta
     6301 gtagctatgc attggttttc ttcgttgagg atcaggcgag tgacccgatc taagaagcgt
     6361 ttgactcctt caatacccgt agctgaccag ggtttttcgc tttctaaagg acccataaaa
     6421 caaacgaagg tgcgaagtgc atccgcacca tatttttgac tgacttcatc cgccgcaatt
     6481 ccattatttc gagatttcga catcttctga tggtcttcgc ccaaaatcat accttggtga
     6541 gccagctttt tgaaaggctc atcatgggtt accaggccgg catcaaaaag aactttcatc
     6601 caaaaacgtg catacag
//
LOCUS       DATBTE010000201         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1392735, whole genome shotgun sequence.
ACCESSION   DATBTE010000201 DATBTE010000000
VERSION     DATBTE010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392735"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..916
                     /locus_tag="VIG33_07450"
     CDS             182..916
                     /locus_tag="VIG33_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8270709.1"
                     /translation="MNETATLITGSNGGVGQFITKYLLEKGHRNLVCHYRSKNDQVIT
                     LLKAFDLDPAKHSVQADLSDENSISRMAQQIQINFKFVEKVVNVAGSSTNALSWKMTK
                     QDFMHVLEDNLLTSFLCSKEFIPNMREQKRGRIVNFSSIVGFTGVAGAAHYCAAKAGL
                     VGLTKSISLELASKGITVNAIALGYFNAGLLESVPAEMKQEILKKIPMGRFGTGQDIG
                     AAVEYLLSDSAQFFTGQVLHLNGGQY"
     gene            916..1989
                     /locus_tag="VIG33_07455"
     CDS             916..1989
                     /locus_tag="VIG33_07455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8270710.1"
                     /translation="MEIVYRKFHKETDYAEQRKLFRLSFPETEGTPTITDAHFNWKFE
                     SYPANKAKTPSYQYVALDEEIVGYYAALPYQYKIGDNIMTCGMVCDVMTHPQRRGRGI
                     FTSIGHHATNDLKEKGVSFTTGYPIRPEVIPGHLKVGWKVTLKLPMYLRPLRLKSFLP
                     KKVQFLSGIVSPFISLIQFFALLPKKYFTYDILNKSDFLKLAEYDGFFNDWLKNQSNA
                     LIKSKSFLNWRTSAPGTEYQFVILRHKEKLAGLALARGVNLKGIESLAVLDIMILPEF
                     YRGARVLHQGLRKLALQLQKDVVVCMASSYWTRNYKLRTSFYLPTPAIFSLIVKKLDL
                     SIPDRNLFSPDNWHLFWVDSDDL"
     gene            1986..>2405
                     /locus_tag="VIG33_07460"
     CDS             1986..>2405
                     /locus_tag="VIG33_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEY8270711.1"
                     /translation="MKLAAYILPHQDDESFLIPKIRSDIADEAEPIFYFLTKSAISEE
                     MASTRQSESKEFLSLLGVKEKNILFLGQTHSILDGTVVDHLSLLKTKLSILIQKHIDS
                     TNATSIEIICPAFEGGHHDHDAAFILSRALAKQFNGPT"
BASE COUNT          744 a          492 c          489 g          680 t
ORIGIN      
        1 cggatccagg caaagaaaat agtcttccaa tactaattca ctgtggcatc atatggtaaa
       61 taacttgtcg gaatttcatt ctttcttata ttttacgagc accattcaga gctcaagata
      121 aactgaagta ccgattattt actcagatat aaaaaacaca tgatttaaaa taggagaaat
      181 aatgaacgag acggcaactt taatcacggg ctcgaacggt ggagttggac aatttatcac
      241 taagtatctt ttggaaaaag ggcacagaaa cctcgtctgt cattaccgct ctaagaatga
      301 ccaagttatc actctgctca aagcatttga tcttgatcct gcaaagcaca gcgttcaggc
      361 agatcttagt gatgagaata gtatttctcg catggctcag cagatacaaa taaattttaa
      421 atttgtcgag aaagtcgtca atgtagcggg ctcctcgaca aatgccctct cttggaaaat
      481 gacaaagcag gacttcatgc atgttttgga ggacaacctt ttgacctctt ttctttgcag
      541 caaagaattt attccaaata tgagagaaca aaaacgagga cgtattgtta atttctctag
      601 tattgtcggc tttacgggtg tcgccggggc cgctcactat tgtgccgcca aagcggggct
      661 ggtgggtctt actaaatcca tatcattgga gctagcctcc aagggaataa cagtgaatgc
      721 cattgcactg ggatatttta acgctgggct actggaaagt gttccggctg aaatgaagca
      781 agaaatttta aagaaaattc ctatgggccg attcggaaca ggacaagata taggcgcggc
      841 ggtagaatat ctcctcagtg actctgctca gttctttaca gggcaagttc ttcatttgaa
      901 tggcggacag tattgatgga aattgtttac cgcaaatttc acaaagagac ggactacgcc
      961 gaacaacgta aactatttcg tcttagcttc ccggagaccg aaggaacccc cacgatcaca
     1021 gatgcccatt ttaattggaa atttgaatct tatccggcaa ataaagcaaa gactccctcc
     1081 taccaatatg tagcgctaga tgaagaaatc gtcggctatt acgcagcctt gccttaccaa
     1141 tataagattg gtgataatat tatgacttgc ggaatggtct gtgacgtgat gactcacccc
     1201 caacgacgcg gccgcgggat cttcaccagt ataggacatc atgcaacgaa cgatttaaag
     1261 gaaaaagggg tgagctttac cacgggttac cctatacgac ccgaagtaat acctggacat
     1321 ttaaaggtgg gatggaaagt gacattgaaa ttaccaatgt atttaagacc tttaagatta
     1381 aagtcttttt tgcctaagaa agtacaattc ttatctggaa tagtctcacc atttatttct
     1441 cttatacagt ttttcgcctt actgcctaaa aaatatttta cctacgatat tttaaataaa
     1501 agtgattttc taaaactagc ggaatacgat ggttttttta acgattggct aaaaaatcag
     1561 tctaatgcgc ttataaaatc caaaagtttt ttaaattgga gaaccagtgc cccgggcaca
     1621 gaataccagt ttgtcatact gagacataaa gaaaagcttg ctggattagc ccttgccaga
     1681 ggcgtaaact tgaaagggat agaatcactc gctgtcctgg acatcatgat cctgccagaa
     1741 ttctacaggg gcgctcgtgt cttacatcaa ggtttgcgaa aactcgcgct ccagttgcaa
     1801 aaagacgtgg tggtttgcat ggcaagttct tattggacaa ggaattacaa attaagaact
     1861 tccttttatt tacccacacc ggctatattc tcattgattg ttaagaagct cgatttgagc
     1921 attcctgaca gaaacctttt ttctccagac aactggcatt tgttttgggt ggattcagac
     1981 gatctatgaa attagctgcc tatatactgc cacatcaaga cgatgaaagc tttttaatac
     2041 ccaagataag aagtgatatt gccgatgaag cggagcccat tttttatttt ttaactaaaa
     2101 gcgccatctc cgaagaaatg gccagcacca ggcaaagtga aagcaaggag tttttatccc
     2161 ttcttggggt taaagaaaaa aatattctgt ttttaggtca gacgcactct attctggatg
     2221 gcactgtagt ggatcatctt tccctattaa aaacaaaact cagcattctt attcagaaac
     2281 atattgactc aacaaatgcc acttctattg aaattatttg ccccgcattt gaaggcggcc
     2341 atcatgatca cgacgccgcc ttcattttat caagggcttt agcaaaacaa tttaatggcc
     2401 ctacg
//
LOCUS       DATBTE010000202         1839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2006977, whole genome shotgun sequence.
ACCESSION   DATBTE010000202 DATBTE010000000
VERSION     DATBTE010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2006977"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VIG33_07465"
     CDS             <1..390
                     /locus_tag="VIG33_07465"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8270712.1"
                     /translation="PSDVRYLPLASESVDIILSHKLYNNLHLADTLKSISEIARVLTV
                     GGEARLSDISDLAFKYIDKFLQQNYSKELVWKYKGLLVIMEKRQTIKNEVPMIPFDSR
                     FRYQGIPNGTRTFELPWAEGETPDHEP"
     gene            complement(420..>1839)
                     /locus_tag="VIG33_07470"
     CDS             complement(420..>1839)
                     /locus_tag="VIG33_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8270713.1"
                     /translation="ENPQNVIRELLSFPEVEFAERNTMIELFQSPNDDQYLTQWGLHN
                     SGQPNEWGIQGLSGVDINAETAWNLSTGSKNVIVAIIDSGMNYNHPDLKENAWVNIAE
                     LNGKPNVDDDSNGFVDDIYGYDFFNNKADPLDDNGHGTHIAGIIGANGNNKYGVSGVN
                     WNVKIMPIKFTNNKIQGSLEGAIQGIQYAVKMGAHIINASWGSENASEILSSAIREAG
                     EKNVLFIAAAGNNSRDNDSKITKPVYPASFNLPSIISVASIDQKGGLSNFSCFGAERV
                     HLAAPGENIWSTWIDGSYKKQSGTSMAAPFVAGVAALLLSHEPNLTAIELKERLIKTA
                     APLKNLKGKVLSQGRVDAGFALLNQTPPLDPNDPDFWNFQDIKISTNHPYRESEKTFW
                     EINIPGAKALSVYFDKFALANEFDFVDIHDRSGRLISRLYGKSVHGWSQITVGDYAKI
                     TITSDQAVEDYGFDITKVAFKN"
BASE COUNT          547 a          367 c          352 g          573 t
ORIGIN      
        1 ccatcggatg tgcgatattt gcccttagcc tctgagtccg tggatattat tttatctcat
       61 aaactttata ataatttgca tctggcagac accttaaaat caatatcaga aattgccaga
      121 gtattaaccg tgggtggtga agctcggtta agcgatattt cagatttggc atttaagtat
      181 atagataagt ttcttcaaca aaattattca aaagaattgg tatggaaata taaaggttta
      241 cttgtaataa tggaaaaaag acaaactatt aaaaatgaag tgccgatgat accctttgat
      301 tcaaggttca gatatcaagg aatccctaac ggaactcgaa cctttgaatt gccctgggca
      361 gaaggcgaaa ctcctgatca tgaaccttaa ctaattattt aaaaactgaa ctgatcagtt
      421 cagtttttaa aagcgacttt tgtaatatca aatccataat cttcaacggc ttgatccgaa
      481 gtgattgtga ttttagcata gtctcctacg gtgatttgac tccaaccatg aacactcttg
      541 ccatagagtc gagatatcag acgtccagat ctgtcgtgga tatctacgaa atcaaactca
      601 ttcgcaagag caaatttatc aaagtaaacg gagagggctt tcgctccagg aatattgatc
      661 tcccaaaaag ttttttcaga ttctctatag ggatgatttg tcgatatttt gatatcttgg
      721 aaattccaaa aatcaggatc gttcggatct aagggaggtg tttgatttag caaggcaaat
      781 cctgcatcga cacgtccttg agacaggact ttgcctttta gattttttaa aggagccgct
      841 gttttaataa gccgttcttt taattcaatc gcagtgagat ttggttcgtg actcaggaga
      901 agggcagcca ctcctgcaac aaaaggtgct gccatggaag tgccactttg ttttttataa
      961 cttccgtcaa tccaagtact ccaaatattt tctccagggg cggctaaatg gactctttcc
     1021 gcaccgaagc aactaaaatt agaaaggcct cctttctggt caatagaggc aacacttata
     1081 attgaaggaa gattaaagct agcggggtaa acgggttttg taatctttga atcattgtcg
     1141 cgagaattat ttccagcggc tgcaataaat agaacatttt tttcaccggc ctcacgaatg
     1201 gcagaagaaa gaatttctga ggcgttctca cttccccagg aggcatttat aatatgagca
     1261 cccattttca ccgcatattg aatgccttgg atcgccccct caagactgcc ttggatttta
     1321 ttattggtaa atttgatcgg catgattttg acattccaat tcacaccgct tacgccatac
     1381 ttattatttc cattggctcc gataatccct gctatgtggg ttccatgccc gttatcatct
     1441 aatggatcgg ctttattatt aaaaaaatcg tatccataaa tatcatcaac aaacccattg
     1501 gaatcatcat ccacatttgg ttttccgtta agttcagcaa tgtttaccca agcgttttct
     1561 ttcaagtcag gatggttgta attcattcca gaatcgataa tagcaacaat aacattttta
     1621 gatcctgtag aaaggttcca tgcggtttcc gcatttatat ccactcccga aagtccctga
     1681 atgccccatt cattaggttg tccagaattg tggagtcccc attgagtaag atattgatca
     1741 tcattggggg attgaaaaag ctcaatcata gtattgcgtt cagcaaattc cacttctgga
     1801 aaactgagta attctcggat cacattttgt gggttttca
//
LOCUS       DATBTE010000203         6802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_162410, whole genome shotgun sequence.
ACCESSION   DATBTE010000203 DATBTE010000000
VERSION     DATBTE010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162410"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VIG33_07475"
     CDS             complement(<1..559)
                     /locus_tag="VIG33_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8270714.1"
                     /translation="MKNQKSEKMTLVAHRILFLCERSGVDSVTMSRVARMAEVSRPWL
                     YKYIGQTKEDLVSFAFDHYGNEFALLNSRPNPRNFEEWMNSLESGFDHLLQMGRKNPW
                     ALSLYFRFKGHSGSVGEKVRKIENQFIKKSAEEMTKALDFPKPQALEKARLINSVRLA
                     LAHDVLINPKSKGLEIISKDFFCQLC"
     gene            705..1574
                     /locus_tag="VIG33_07480"
     CDS             705..1574
                     /locus_tag="VIG33_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270715.1"
                     /translation="MKINLMTTGFAFLLTTTGMKAQAQSAQPQIDFTAVNGVIQEVIT
                     SSVKLDNVIKGVTLVIDPSSNLPQGKIDAKFAAAAGMTPWDKTQDSQISLQVGSRFKD
                     VNTLSKEGALKLKVQAKTPVLEMIRYFAAKEIEHASPQYPHFYDATIKKLSEVKDIAE
                     LYEIAVELKQLLIENADNQEEKEFIQSLRFTAKTVDGVVESFKVEETKPLVLNFAGNI
                     EIRMAAITINPKSMGAGFILATTMDTTMFNQYVEKSTAFFAELQGRNVQYLDYIKDTV
                     SDYMDLVRDFLNQ"
     gene            complement(1647..2108)
                     /locus_tag="VIG33_07485"
     CDS             complement(1647..2108)
                     /locus_tag="VIG33_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270716.1"
                     /translation="MLKQAISSGVESTIFANQDMAGTNQSNSKQRVMDCTTKYQTIES
                     RLSNNNRFRFSVVKLIEDNHGVSVLPLYLDGVKVLENLGGGVTVGTTIEKLQRNRGAS
                     GGGSLIMGYIDAETKGTFTIAPSTKGKIEVSISIENFTNGANPFQEVLVCK"
     gene            complement(2243..3229)
                     /locus_tag="VIG33_07490"
     CDS             complement(2243..3229)
                     /locus_tag="VIG33_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270717.1"
                     /translation="MRHLIYSTLIFLMSLAQMGCGGNGAAATFDLDQTGQNFGQKILY
                     NNKVDIVFIVDNSTSMAQHQNRLNAQVPTLVSILKSLKMDYHIVSISTSMGLGGNGGT
                     FLGSPMILDSQTANLESLLQQKIALGDTGSNNERGLFSLETALSPAYLSSEGAGFLRE
                     DALLAVIELSDEDDKSGKTASYYANLLDKLKPATTNGRKSWIFNFIGVLENSSVCRTF
                     NDYSEVGTTLMQLADLSGGNKESLCSNNLAAAVTNIKARIVQILTDFYLKSRPVKETL
                     KIAVNGKLVSESATNGWTYIEDGTKYIIQFHGEAIPPADADIRVDYTPTNNT"
     gene            complement(3256..4050)
                     /locus_tag="VIG33_07495"
     CDS             complement(3256..4050)
                     /locus_tag="VIG33_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HEY8270718.1"
                     /translation="MQTLFYVSLNGGHVGPYSVETIVQKIEAGEHTWTDYVYCEAKAD
                     WVVAMEHPSFADKFQSSSVDEHTVINAAVPHHDEKYKDKAWYLLKEGNNYGPFSKLEL
                     VQMLQEKTLFEYDYVWHSKLNAWKRIAELEEYSPDSIRAMKESGLAEIAEVFFRRRHA
                     RASYGCSLIVHNNKTVFKGRSMEISSGGAGILIDNPNLQPGQNLFLHFQPGDGVPPFN
                     AVCTVVSKQMVKTTKNALDSVKYGVRFTSISQSVRESIRQFTQKVA"
     gene            4304..5125
                     /locus_tag="VIG33_07500"
     CDS             4304..5125
                     /locus_tag="VIG33_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8270719.1"
                     /translation="MTESKSFPYLHGFSEEEQQRLRKQAQFGEYMVYQNINLSNVTDL
                     LEIGCGVGAQSEIILRRFPDLKLTGIDRSSKQLSMAQKMLSKAPYTSGRFKFKEMDAT
                     AMEFSANSFDGAFLCWILEHVPDPIRVLSEVRRVLRPASTVYITEVMNSSFFLDPYSP
                     NVWKYWMAFNEYQLAQSGDPFVGAKLGNLLMQLGYHDIHTEIKTWFLDNRYPQARKDC
                     VEYWEDLLLSASQQLVEANYVSDEVVEEMKKEMSKVANDPNAVFFYSFVQASAKT"
     gene            5278..5844
                     /locus_tag="VIG33_07505"
     CDS             5278..5844
                     /locus_tag="VIG33_07505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY8270720.1"
                     /translation="MKILMFAASFRKDSLNKKLIRNAHQMMPAGHELTLKEFNEYTMP
                     LYHGDLETTSGIPDAAQKLIQDIAGADAVVISSPEYNAGIPGTLKNAIDWTSRVKPVP
                     WQGKQILLMGASPGMIGTNRGLWQVRQPLEYLGGFVFPEMLGVALADKAFDEKDLLKE
                     PRMHESLQKLLNKFVDYASYASLKKLSQ"
     gene            complement(6122..>6802)
                     /locus_tag="VIG33_07510"
     CDS             complement(6122..>6802)
                     /locus_tag="VIG33_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270721.1"
                     /translation="NKDAIKTGTYKGQFKDLQPANLVFKFTIDDNKVLKLDITEYGRM
                     EYDLKSRETKYLDERRRQSFIIKDFGSVSWVTKGSADKNIVVRFLPQFSEDDPKKLTY
                     TPLAFSDAVMTDNGGRVWIEGSGAAGEVSGFITPYGTLYLSFIEFQGSKELGTAKDEA
                     ITLQLNDKMTVRLRSSKPVIGPGYVAKVYGIYFPERKGPATVTSMTTETLEKQLKGMP
                     SSESRYRN"
BASE COUNT         1905 a         1540 c         1440 g         1917 t
ORIGIN      
        1 aacatagctg acaaaaaaaa tctttggaaa ttatctctaa tcctttagat ttcggattta
       61 tcaaaacatc atgagctaaa gccagcctta cagaattaat cagtcttgct ttttctaagg
      121 cctgcggttt tggaaagtcc agggccttgg tcatctcttc cgcagatttt ttaatgaatt
      181 ggttttctat ttttcttact ttttcgccaa ctgagccact gtgtccttta aagcgaaaat
      241 acaaagacag agcccatgga tttttccgac ccatttgcag cagatgatca aaacctgatt
      301 ctaaggaatt catccactct tcgaaatttc gtggattggg tcgagagttc agcaaggcaa
      361 attcatttcc atagtgatca aaggcgaatg aaaccagatc ttctttggtt tggcctatat
      421 acttataaag ccagggccga gagacctcgg ccattcttgc aacgcggctc atggtcacag
      481 aatcgacacc gcttcgttca cagaggaaga gaattcgatg ggcaaccaag gtcatctttt
      541 cgcttttttg atttttcatc gaaagcctcc tgacgattga ctgcgattga aatgatgcct
      601 tatgtaaata aagttacagt tatccccaaa agtcgtcacc catttttttt gcttcccccg
      661 ctgtgcagga ctatatccct cttaaacaaa aaaaggcggt atctatgaaa ataaatttaa
      721 tgacgacagg atttgcattc cttctaacaa caacgggcat gaaagcccag gctcaatcgg
      781 ctcagcctca aatcgacttc acagcagtga atggcgttat tcaagaagtg atcacaagct
      841 ccgtaaaact ggataatgtg atcaagggag tcactctggt catcgatcca tcttcaaatc
      901 ttccgcaggg aaagattgac gcgaaattcg ccgcagctgc tggcatgact ccttgggata
      961 agactcaaga tagtcaaatc agccttcaag tcggaagtcg atttaaggat gtgaataccc
     1021 tttctaaaga gggcgcatta aaactgaagg tgcaagctaa gacccccgtt cttgaaatga
     1081 ttcgctattt tgcggctaaa gaaatagagc atgcgtctcc acagtatcct catttctacg
     1141 atgcgacgat caaaaaatta tctgaggtta aggacatcgc tgaactctat gaaatcgcgg
     1201 tcgagctcaa gcagttgtta attgaaaatg cggataacca agaagaaaaa gaattcatac
     1261 aaagcctcag attcacggcg aaaacagtcg atggtgtggt tgagtccttc aaagttgaag
     1321 aaactaagcc cctagtcctt aattttgctg gtaacattga aatcagaatg gccgccatta
     1381 caataaatcc gaaatctatg ggtgcaggtt ttatccttgc cacaaccatg gatacaacca
     1441 tgtttaatca atatgtcgaa aaaagcactg cgttctttgc tgaacttcag ggtcgtaatg
     1501 tacaatattt ggattacatc aaagacacgg tcagcgatta tatggacctt gttcgggatt
     1561 tcttaaatca ataacacgta caacagtcta taaaaagaaa tgggccagcg gtctcagaag
     1621 agaccctggc ttttcgagcg taatctttat ttgcaaacca aaacttcctg aaaaggattt
     1681 gcgccattgg tgaagttctc gatagaaata gaaacctcga ttttaccttt tgtgctgggt
     1741 gcgatagtga atgttccctt cgtttcagca tcaatataac ccataatgag tgaccctcct
     1801 cctgaggctc cgcgattcct ctgaagtttc tcgattgtag ttccaacggt aacgccaccg
     1861 cctagatttt ccagaacttt cacgccatcg agatacaagg gaagaacact gacaccgtga
     1921 ttgtcttcaa tcaattttac aacggaaaat cggaagcgat tattgttaga aagacgagat
     1981 tcgattgtct ggtatttagt tgtgcaatcc atgacccttt gcttagaatt tgactgattc
     2041 gttcctgcca tatcttgatt tgcaaagatt gtactttcca cgcccgaaga gatggcttgc
     2101 ttcaacatgg acaagcacga ccgtttaaac tcaatcgcgt attcgctact gagttgcaca
     2161 gtacgctact cagcttaagg tatataagga ctcactaagc aatagagtta tccattttat
     2221 tcaaactcat tgggacgttg atttaagtat tattcgttgg agtgtaatcc acgcggatat
     2281 ccgcatccgc cggaggaatc gcttcgccgt ggaattgaat aatatatttg gtgccgtctt
     2341 caatgtaagt ccaaccgttt gttgcgctct ctgaaaccag cttgccattc accgcgatct
     2401 taagagtttc tttgactggg cggctcttca aatagaaatc cgtgaggatc tgcacgatgc
     2461 gtgctttgat gttcgtcacc gctgctgcca aattgtttga acagagcgat tctttgttac
     2521 ccccagaaag atccgctaat tgcatcaatg tggtgccaac ttccgagtaa tcgttgaaag
     2581 tccgacaaac actggagttc tcgagcactc caataaaatt aaagatccag gattttcgac
     2641 cattcgtggt ggcgggtttt agtttatcta ataaattcgc gtagtagctg gcggtcttgc
     2701 cgctcttgtc atcttcgtcg ctaagttcaa tcaccgctag cagcgcgtct tcgcgtaaaa
     2761 agcctgcgcc ttcagaggag aggtatgccg gtgacagtgc tgtctccaat gagaaaagac
     2821 cacgttcatt attgctgcca gtatcaccca aggcgatttt ttgttgcaga agactttcca
     2881 aatttgcggt ttgcgaatcc aaaatcatgg gcgatcccaa aaaagttccg ccgttgcccc
     2941 ccaaccccat gctggtggag atggatacga tatgataatc catcttcagg ctttttaaaa
     3001 tcgacacgag cgtaggaact tgggcattca agcgattttg atgttgtgcc atagacgtgg
     3061 aattatccac gataaacacg atatcgactt tgttgttata aaggatcttc tggccaaaat
     3121 tctgaccagt ttgatccaag tcaaaagttg cggcggcacc attccctccg caacccattt
     3181 gcgccagaga catcagaaaa atcaaagtag aatatattaa atgtctcacg aaacccctcc
     3241 ctgagagaga ccgccttaag cgactttttg cgtaaattga cggatggact cccgcaccga
     3301 ttggctgatg ctagtaaacc tgacaccgta cttgactgaa tccagagcat tctttgtggt
     3361 cttaaccatt tgcttgctca ccacagtaca aactgcattg aagggaggga caccatcgcc
     3421 tggttgaaaa tgcaaaaata aattttgccc tggctgcaga ttcggattgt cgatcaaaat
     3481 tcccgctcca cctgagctga tctccatgct gcgaccttta aaaactgttt tgttgttatg
     3541 aacaattaaa gaacagccat aagaagcgcg cgcatgacga cgccggaaaa aaacctccgc
     3601 gatttccgct aatccggatt ctttcatggc acgaatggaa tctggtgaat actcttcaag
     3661 ctcggcgatg cgtttccagg catttaattt tgaatgccat acataatcat attcaaacag
     3721 agttttttct tgaagcattt gtacgagctc caactttgag aaagggccat agttattgcc
     3781 ctctttcagt aggtaccaag ccttgtcttt gtatttttca tcgtgatgag gaaccgcggc
     3841 attgatgaca gtatgctcgt caactgacga agattgaaac ttatccgcaa agcttgggtg
     3901 ctccatggct acaacccagt cagctttcgc ttcacaataa acgtagtcag tccaagtgtg
     3961 ttcaccggct tcaatttttt gtacaattgt ctctaccgaa tagggcccca catgaccacc
     4021 atttaaagag acgtaaaaaa gcgtttgcat actgcacctc atgttcattg acttcccgaa
     4081 ctctatcggc aggggccgga ccagacttaa gaggacaaaa tcaaaagact tattcgtcgg
     4141 tttcatttcc ccgaaaccgt ctaagttttt tacaggactc attcgaaaaa ttccaaatat
     4201 cgaaaaagtt ttagacagaa ataaggatct ctaatgttgt gttgcgacac caagactcag
     4261 gacttttgac gtttcccctg acttatgagc tagactctag agtatgacag aatctaaaag
     4321 ctttccttat ttacatggat tttctgaaga agaacaacag cgtctgcgta agcaagctca
     4381 gtttggcgag tacatggttt accaaaacat caatctttcc aatgtcacgg acttattgga
     4441 gatcggttgt ggcgtcggcg ctcaaagtga gattattttg cgccgctttc cagatttgaa
     4501 actcaccggt attgatcgca gctctaagca attaagcatg gcccagaaaa tgctttcgaa
     4561 agctccctat acctcgggac gctttaagtt taaagaaatg gatgccaccg ctatggagtt
     4621 ttccgccaac tcttttgatg gggctttcct ttgttggatt ctagaacacg tccccgatcc
     4681 cattcgcgtg ctctcagaag tgcgccgtgt ccttcgaccc gcctcgactg tttacattac
     4741 tgaggtcatg aattcttcat tcttcttaga tccctactct ccgaatgtgt ggaaatactg
     4801 gatggccttt aatgagtacc aactcgcgca atccggtgat cctttcgtcg gcgccaagct
     4861 cggcaattta ctgatgcaac ttggatatca tgatattcat acggaaatta aaacctggtt
     4921 tctggacaat cgttatcccc aagcccgcaa agactgcgtc gagtattggg aggatttact
     4981 cctcagtgcc agccaacaat tggtggaggc aaactatgtc tctgatgagg tcgttgagga
     5041 aatgaagaag gaaatgtcaa aggtcgcaaa tgaccccaac gcggttttct tttattcttt
     5101 tgttcaagcc agcgccaaga cttgaaatta tgacctcatt ttcatccatt tatggacatt
     5161 tttggcgttg tttttgacca taaaatgata acaatgagat ctgtttaaca gaaatgtcta
     5221 gaattagaaa tagcccatac acagttttct tttattctaa gctatccagg agcgcttatg
     5281 aaaatactca tgttcgcggc ctcttttcgc aaggactccc ttaataaaaa actgattcgc
     5341 aatgcgcatc aaatgatgcc tgcggggcat gaacttactc tcaaagaatt caatgaatat
     5401 acgatgcccc tttaccatgg ggatcttgaa acaacttctg gcattcccga tgcggctcaa
     5461 aaactcattc aagatatcgc gggcgccgac gccgtggtga tttccagccc cgagtacaat
     5521 gcgggtattc ccggaacttt gaaaaatgcc attgattgga cttcccgtgt gaagcccgta
     5581 ccctggcaag gaaaacaaat cctgcttatg ggcgcttcgc cgggaatgat tgggaccaat
     5641 cgtggcctat ggcaagtgcg ccaacctctg gaatatctcg ggggttttgt tttcccggaa
     5701 atgttaggag tggcgttggc agataaggct ttcgatgaaa aagatttgct gaaagaaccc
     5761 cgtatgcacg agtcactgca gaagcttttg aataaatttg tggattacgc gagctacgca
     5821 agcctaaaaa agctctctca atgaaacgga tatatccagg aagtcgcagg atcggaaact
     5881 ggcactgaag gctcccgggg tttattgcaa tccgtaaagc tcatgaatag gggaataaag
     5941 aacaaaaaga ctctcataaa aactcctcag gggcacccag attctacctt gagagtctaa
     6001 gctaaagttt ccggtaatga tgacgattta gcgaacttcc tcatatatct ccagcgggag
     6061 accatccgga tcttgaaaaa aggtaaaacg ttttccggta tattcatcgg tgtgcatagg
     6121 ttcaatttcg atatcgtgac tctgaagacg gcatgccttt caattgcttt tcgagagttt
     6181 cagtagtcat cgaagtaaca gtcgctggtc ccttgcgttc agggaaatat attccataaa
     6241 ctttcgcaac ataaccggga ccaataactg gtttggaact ccttagcctc accgtcatct
     6301 tatcattaag ctgtaaggtt atcgcctcat ctttcgcagt tcccaattcc ttggaccctt
     6361 gaaattcaat aaagcttaaa taaagggttc cgtagggtgt gataaaaccg gatacttccc
     6421 cagcggctcc ggagccttcg atccagactc ggcccccgtt gtcagtcata acggcatcag
     6481 agaaggcaag tggggtatag gtcagctttt ttggatcatc ttcagaaaac tggggtaaaa
     6541 accgaacgac tatattttta tcggcagagc cctttgttac ccaagaaacg ctcccaaaat
     6601 ccttaataat aaaggattga cggcggcgct catctaagta tttggtttca cgggatttta
     6661 aatcatattc cattcttcca tactcggtga tatcgagttt gagcacttta ttatcatcta
     6721 tggtgaattt gaaaacgagg ttagctggct gtagatcttt aaattgtccc ttataagtac
     6781 ctgttttgat agcgtctttg tt
//
LOCUS       DATBTE010000204         9436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_299996, whole genome shotgun sequence.
ACCESSION   DATBTE010000204 DATBTE010000000
VERSION     DATBTE010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9436
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299996"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VIG33_07515"
     CDS             complement(<1..326)
                     /locus_tag="VIG33_07515"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY8270722.1"
                     /translation="MSSLSKLNRNRTPPFDGIKKASELVHKLASEMALHQAYLFGSAA
                     NNEHTLDSDLDILVVVESTSDIAKLYKIVQRPFFSSVAVDWIVKSRAEFSKMKDVGGV
                     CFVAFH"
     gene            complement(388..894)
                     /gene="sixA"
                     /locus_tag="VIG33_07520"
     CDS             complement(388..894)
                     /gene="sixA"
                     /locus_tag="VIG33_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEY8270723.1"
                     /translation="MELILMRHGLAEDREEFAKKNLEDSARPLTMKGRKRTQKVAIQL
                     RDWMEDMDLIVTSPFVRARQTADLVSQIFIDTPVVEAAELVPTSLPQSFKRWLRSHGE
                     DHKRIMAVGHEPHLSALASFLMANQLEPLLEIKKSGVLCLNIESFKEIAPGSAKLLWL
                     VQPRQMVD"
     gene            complement(904..3078)
                     /gene="ppk1"
                     /locus_tag="VIG33_07525"
     CDS             complement(904..3078)
                     /gene="ppk1"
                     /locus_tag="VIG33_07525"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY8270724.1"
                     /translation="MNSTNPTNSKKVTGKKRSARKPKIIEHPLSSDKLFTNREIGWLR
                     FNRRVLSEAEDTRNPLLERLRFLSISSSNMDEFFMKRVGGLKRQVVYGISPKSADGQT
                     PAQQLAAIRQVVLPTIQEQDRCFEKVLKPALAENGIFLLKWAELTLKEKELVKTYYHK
                     NVFPVLTPLSVDPGHPFPFISNLSTSLGVTLKHPERDEKLFARVKIPKVLPQWIRANP
                     EAATFRFVNLVEVIRENLGELFPSMQVISAMAFRLTRNADAEHDQEDTEDLLEMIEEE
                     LRQRRFAEVVRLEHGPNPDPWMIQFLMEELELSEEYIYELRGELDYTDLNMIHDLNLP
                     KLKYEPWTPVVAPALAVEDANSFFAAIKLQDHLLHHPYESFSATVEKFIRLASSDPKV
                     LAIKMTLYRTGDNSPFIKSLIRAAEAGKQVVCLVELKARFDEERNIYWAQELENAGVH
                     VVYGVVGLKTHAKTSLVVRQEAEGLHCYCHIGTGNYNVTTSRFYTDLGLLTAREEITN
                     EIVEFFHYLTGRSLKSSYENLLIAPVNMFPRFKAMIEREGEHAKAGRPAQIIAKFNNM
                     EENDIAMSLYQASQKGADIDLIVRGFCCLRPKVPGLSERLRVTSVIGRYLEHSRIFYF
                     RNGAQDPLDGNFYMGSADWMYRNLHARVEAVVPIIDRALKEKIWEILQLTLKDQRQTW
                     QMTSDGEYTQRKSNDSGVQQVLMQLTKQRVKNFEENNSVGDS"
     gene            3148..4140
                     /gene="thiL"
                     /locus_tag="VIG33_07530"
     CDS             3148..4140
                     /gene="thiL"
                     /locus_tag="VIG33_07530"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEY8270725.1"
                     /translation="MQNTPREHNLIQKLKKLVRRDDPRTLVALGDDAFVFRSFTESTV
                     ITQDMLIENVHFKMNYFGPSDLGHKALAVNLSDLAAMGAKPHFAQVSLALPKDVTEDW
                     VLSFYSGMLHLADQYNMEIVGGDLNQSPGPVVIDVSLVGEVSQSFRRSGAQAGDWLAV
                     TGPLGLSHTGRMALEKELYIYAEATQKHLRPQPRMDVVTLLRQHQLTSKTIHALMDCS
                     DGLINDLLHLTRNCELGVELKEEDLPLASDTEALARSLNKKPLDWALWGGEDYELLMA
                     FPPNKKDEMKYIFESADLDLFLIGKFNDSGKITLATHHGKVEEIMEFKGWTHFS"
     gene            4196..4732
                     /locus_tag="VIG33_07535"
     CDS             4196..4732
                     /locus_tag="VIG33_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY8270726.1"
                     /translation="MSQNLARYHARSPRYILNTDDDSLIRFAGPRQTPWEEGTDIKNV
                     SLTGLAFTAPDDLCPVLGEIVKIQFMAPGGKQMASYAFVTRLEPIDKGRILVGLHFYK
                     MEMAHRIVLAQGLARKLKDISDREKILSLQEDPAFSFSAFFPHGFLMISYFVIWMVIM
                     HLLMQENWLQIFNSWLNF"
     gene            4887..6344
                     /locus_tag="VIG33_07540"
     CDS             4887..6344
                     /locus_tag="VIG33_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY8270727.1"
                     /translation="MVIKWFISLFLSTQALALPADLSCKPNLTLSQKDVAELILKQGR
                     RTQEINLQYRQFRLSVAQSLAAYQWNLLATTGYLFDKSVGLQNSAFDKTENLTTNVKL
                     SKNFTTGTIASLEYKRISFKGDPNALTSVTSRSIATQDLLGLNIAQNIWANFFGIADR
                     GEIDAADFNYKSSAILRANELENLVLDSIRTFWTAYVSEQNFQQTMVSRDRSKKLVDA
                     VKKKSGFGYANPGELAQAQADYEGRIQEVKNTSAAYLANLDTLNTLLSLPQGCEVKFS
                     ISNVVPTIPQLPEKSVQDLRVIRSEKLKVQGAQRAYDAAKSKDAPTLQLIGKIYSYGL
                     QSDGNGAYSDMVSGAHPQYYAGVQFEYLFGAELLSENRLNKKYVKDLEETRLSRIIQE
                     SEDVQVQAQRKVQTTYAAVLSTSEQKKYREKALQELSRSFNQGRTDISIYIDAMNKFS
                     NAEVQYSQAVGNYQTALNEWAAARDELIPDAAQEE"
     gene            6453..6983
                     /locus_tag="VIG33_07545"
     CDS             6453..6983
                     /locus_tag="VIG33_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270728.1"
                     /translation="MEPQQPEPQRAPKPKIKIPLPQLPAPNKTSIANQVCWYMGAFMV
                     ALSIVGAVIPGLFEAHLNPIHNFILLASGFSSLWFGLKTMDYRAKKFCNWMGGFYMML
                     GVAGFGFGHRALSLTRPTASGIPEESAFLWQLVPGKFELGTVDHFLHMVIGGIFLLGA
                     YFTLKKIRPFRKNIWH"
     gene            complement(7092..8912)
                     /locus_tag="VIG33_07550"
     CDS             complement(7092..8912)
                     /locus_tag="VIG33_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEY8270729.1"
                     /translation="MALLINTHQLEKSFAGKVLFSGVSLGIEDGDKIGLLGPNGAGKT
                     TLMKILAGVSTADGGKVTPKKGLRLGYLPQTPVFAAKETILQALLSRAHDPDEALGLA
                     YEWFARLDLSQFGEDFLVSELSGGWQKRVALARELVLEPELLLLDEPTNHLDVVSILW
                     LEEFLQRAPFAFLMVTHDRLFLQRVVNKVFDLDPRNPNNLLAVNGDYIQYVEAKEQLL
                     ASQQKLETVLKNTLRRETEWLRRGAKARLTKQKARIDRAGDLKEDVENLTDKNRKRVA
                     GIAFGDAERNPQKLIELKNVSKSYGDRHLIQDFSFLITPKTRLALLGENGSGKSTLIK
                     MFLGLEQPDQGSIFLSDKVKVAYFEQNRAGLDLEKSVLKNICPEGDYVSLQGQYVFAR
                     SYLERFLFSRQQMDLPVSRLSGGEQSRLRLAQLMLQDASVLILDEPTNDLDVPTLEVL
                     GEAIQNFNGAVVIVTHDRYFMDQVATDILGFPRQDNPKKKLENFVGYLQWEEAWFAQD
                     QELAAAAVDPTPQIVEEKPAAKVSRMSFKHKNELEGMEPKILAMEDELAKTQAEAASP
                     EVVSKASRVQELHAKMAELQSEIESSYARWAELEKMAKGE"
     gene            complement(8900..>9436)
                     /gene="aat"
                     /locus_tag="VIG33_07555"
     CDS             complement(8900..>9436)
                     /gene="aat"
                     /locus_tag="VIG33_07555"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY8270730.1"
                     /translation="PLLWFSPEKRGILEFSEFHLSRSLEKFLRHYAGDFEVTVDMAFA
                     QVVRECSKQPRPGQDGTWILPSMVKSYINFHKAGYAHSIEVWHERQLVGGIYGVFIKG
                     VFSGESMFYKSSNASKLALFHLVQLLKSWGLEWMDIQMVTPVTKSFGGKYLEREDFLQ
                     KLKRTQDENENSVFAWPC"
BASE COUNT         2671 a         2297 c         1942 g         2526 t
ORIGIN      
        1 tgatgaaatg caacaaaaca aactccaccc acatccttca tctttgaaaa ctcggccctt
       61 gatttgacaa tccaatcaac cgccaccgaa gaaaaaaacg gacgctgaac aattttatac
      121 aatttcgcaa tatcagaagt ggactcgaca acgacaagaa tatctaaatc tgaatccagg
      181 gtatgctcat tattagcagc cgatccaaat aggtaagcct gatgcaaggc catttcagac
      241 gcaagcttgt ggacaagctc agaggccttt ttaatcccat caaaaggtgg agttctattt
      301 cgatttaact tcgataaact tgacatctgt ttatgctacc aaaaagctct tagattttca
      361 gcattttttg tgtctaagat gactctccta gtccaccatc tgacgaggct gcaccaacca
      421 caggagcttc gcggatcccg gcgcgatttc tttgaagctc tcgatattca agcacaaaac
      481 tccgcttttt ttgatttcca aaagaggttc caactggttc gccatcagaa aacttgcgag
      541 cgcactgagg tggggctcgt gccccacggc cataatcctc ttatggtctt ctccatgaga
      601 gcgaagccat cttttgaagg actgcggcag gcttgtgggc actaattccg cggcctctac
      661 caccggagta tcgataaaaa tttgagaaac caaatcggcg gtttggcggg cccgcacgaa
      721 aggactggta acgatcagat ccatatcctc catccaatcc cgtaactgga ttgctacttt
      781 ttgcgtgcgt ttgcgacctt tcatggtcaa tggacgtgca gagtcttcaa ggtttttttt
      841 agcaaactct tcacgatctt ccgcaagtcc atgcctcatt aaaatcaatt ccataaacac
      901 atctcaagaa tctccgacag aattattttc ttcaaaattt tttactctct gcttggtcag
      961 ttgcataaga acttgttgca ctcccgaatc attagacttt ctttgagtat actctccatc
     1021 tgaagtcatt tgccaagtct gacgttgatc ttttaaagtc aactgcaaga tctcccaaat
     1081 tttttctttt aaagcacgat ctatgatcgg tacgaccgcc tctacccgag catgcagatt
     1141 ccgatacatc caatccgccg agcccatata gaaattccca tccagaggat cctgagctcc
     1201 atttctaaaa taaaaaatac gcgaatgctc gaggtatctg ccaatgaccg aggtcacgcg
     1261 caaacgttca ctcagcccag ggactttggg acgaagacaa cagaagccgc ggacgatcaa
     1321 atcgatgtcg gcgccttttt gcgaagcctg atacaaagac atggcaatgt cattttcttc
     1381 catattatta aacttcgcga tgatctgagc cgggcgcccg gctttcgcat gctctccttc
     1441 gcgctcgatc atagccttaa accgagggaa catattcacc ggggctatca aaagattttc
     1501 ataggaactt ttcaaagaac gaccggtgag ataatgaaag aattcgacaa tctcattggt
     1561 aatttcctct cgcgccgtga gcaatcccag atctgtgtaa aagcgagaag tcgttacatt
     1621 gtaatttccc gtgccgatat gacagtaaca atgaagtccc tcggcctctt gacggaccac
     1681 aagcgatgtc tttgcatgag ttttaagacc tacaactccg taaacaacat gaactccggc
     1741 gttttccaac tcctgagccc aataaatatt tctttcctca tcaaaacgcg ccttcaactc
     1801 caccaaacaa acgacttgct taccagcttc cgcggctcga atcagggatt taataaaagg
     1861 agaattgtcc cctgttcggt acaaagtcat cttaatcgct agaaccttcg ggtctgatga
     1921 ggccaagcga atgaatttct caaccgtcgc ggaaaagctt tcgtaaggat gatgcaataa
     1981 atgatcctga agtttaatag ctgcaaagaa ggagttcgca tcctcgacgg ccagcgctgg
     2041 cgccacgacg ggcgtccaag gttcatattt aagcttcggt aaattcaagt catgaatcat
     2101 attgaggtcc gtataatcaa gctcgccgcg aagttcataa atatattctt ctgaaagctc
     2161 gagttcttcc atcaaaaatt gaatcatcca gggatcagga ttgggaccat gttccaagcg
     2221 aaccacttct gcgaaacgac gctggcgaag ttcttcttcg atcatttcca acaaatcctc
     2281 tgtgtcttcc tgatcatgtt cagcatcagc atttcgtgtg agtcgaaaag ccatagcact
     2341 gattacttgc attgaaggaa acagctctcc taaattttct cgaatcactt ccacaagatt
     2401 gacaaagcga aatgtcgcag cctctggatt tgcccgaatc cattgcggca gaactttcgg
     2461 aatcttaacc cgagcaaaga gtttttcgtc tcgctcagga tgctttaagg taacacccaa
     2521 agacgtcgag agattggaga taaagggaaa ggggtgtcct gggtccaccg agagcggagt
     2581 gagtacagga aacacatttt tatgataata ggttttaacc agttcctttt cttttaaagt
     2641 caactcggcc cactttaata aaaaaattcc attttcagcc aatgctggct tcagaacttt
     2701 ttcaaaacag cgatcttgct cttgaatggt cggcaacacc acttggcgaa tagcagccaa
     2761 ttgctgagct ggggtttgtc catccgcact ctttggtgaa ataccataaa ccacctgacg
     2821 cttaagcccc cccactcgtt tcatgaaaaa ctcatccata ttcgacgaag aaatactgag
     2881 aaaacgtaga cgctctaaca aaggattcct tgtatcttcg gcttcactta atacacggcg
     2941 attaaatcgc agccagccaa tctctcgatt ggtaaaaagt ttgtctgaag agaggggatg
     3001 ttcaataatc ttaggtttac gtgcacttcg ctttttgcct gttacttttt tgctgtttgt
     3061 aggatttgta ctgttcatcc ttgacacctc attagattat ttcacaataa aaccgattat
     3121 ttagcaacta aggctcaggt caacaagatg caaaacacac cgcgcgaaca caatcttatt
     3181 caaaaactaa aaaaactggt ccgtcgcgac gacccaagga ctttggtcgc cttaggcgat
     3241 gatgctttcg tattcagaag ctttactgaa agcactgtca ttacccaaga tatgctgatc
     3301 gaaaatgttc attttaaaat gaactatttt ggtccgtcag atcttggaca caaagcccta
     3361 gctgtaaatc tcagcgacct cgctgctatg ggagccaaac cccattttgc tcaagtctct
     3421 cttgcgcttc ccaaagatgt gactgaggat tgggtcttaa gcttttactc cggtatgctc
     3481 catctagcgg atcaatacaa tatggaaatt gtcggaggcg atctcaatca gagcccagga
     3541 cccgttgtca tcgacgtaag tcttgtcggc gaagtttcac aatcctttcg tcgctctgga
     3601 gctcaagctg gagattggtt ggcggtcact ggacctttgg gtctttccca tacaggaaga
     3661 atggctttag aaaaagaatt gtatatttac gccgaggcca cgcaaaaaca cctacggcca
     3721 caaccgcgta tggatgtggt caccctcctg cgacaacatc agctcacttc gaaaactatt
     3781 cacgccctta tggattgtag cgatgggttg atcaatgacc ttcttcattt gaccagaaac
     3841 tgtgaactcg gggtggaact caaagaagaa gatcttcctt tggccagtga cactgaagct
     3901 ttggctagaa gcctcaacaa aaaaccattg gattgggctc tttggggagg cgaggattat
     3961 gagctgctta tggcctttcc cccaaacaaa aaagatgaaa tgaaatatat tttcgaatct
     4021 gcggatttag atttgttctt aattggcaaa tttaatgaca gtggcaaaat cactcttgcc
     4081 actcaccacg gaaaagtcga ggagatcatg gaatttaaag gctggactca tttttcttaa
     4141 agtttcgctg aagtttcgct taggtcgaac tcttgctcta ccgataaaac acgatatgag
     4201 ccagaacctt gcccgctacc acgcgcgatc gccgagatac atcctcaata ctgacgatga
     4261 ttctctcatt cgcttcgcag gtcctcgcca aacgccttgg gaagaaggca cagatattaa
     4321 aaacgtctcc ctcacaggtt tagcatttac cgctcccgat gatctctgcc cggtacttgg
     4381 tgaaattgtt aaaattcaat ttatggctcc tgggggaaaa cagatggcta gctatgcctt
     4441 cgtcacacgc ttagagccca tcgacaaagg aagaatttta gtaggccttc atttttacaa
     4501 aatggagatg gcacaccgca tagttctagc tcagggattg gctcgcaagc tcaaagatat
     4561 ttcagatcgc gagaaaatac tttccctcca agaggatcct gctttttctt tttcagcatt
     4621 ttttccgcac ggttttttga tgatcagtta tttcgttatt tggatggtca ttatgcacct
     4681 tttaatgcag gaaaactggc ttcaaatttt taattcttgg ctcaacttct agtttcggtt
     4741 ccaaaatcca cagagatgat gcaaggttga caataagtag acccaggctt atctgttgaa
     4801 ggttttgctt gaatctctcc gaataccaaa gatattaaga cttctgggat tcaaaaacat
     4861 aatttagaaa tcggaaccga gataatatgg taatcaaatg gtttattagt ctctttcttt
     4921 cgactcaggc cttggctttg cccgccgact tgagttgcaa acccaatctc actctttccc
     4981 aaaaagatgt ggctgaactg attttaaaac aaggccgccg cactcaagag atcaacttac
     5041 agtatcggca atttcgccta tctgtcgctc aatccttagc cgcatatcag tggaatctcc
     5101 tagccaccac gggatatctg ttcgataaat ccgtaggatt gcaaaacagc gcctttgata
     5161 agaccgaaaa cctcacgacc aatgtgaagc tctctaagaa tttcacaact ggtacaattg
     5221 caagtctcga atacaaaagg atttccttta aaggggatcc taatgcgctc accagtgtca
     5281 ctagtcgcag cattgccact caggatttac taggcctcaa tattgcacaa aatatctggg
     5341 cgaatttctt cggtatcgct gatcgtggcg aaatcgacgc tgctgatttt aactacaaat
     5401 cgtccgcgat cttgagagcc aatgaattgg agaatcttgt tctcgattcc attcgaactt
     5461 tctggacagc atacgtttcg gaacaaaatt tccaacagac catggtttct cgggaccgtt
     5521 ctaaaaaact cgtggatgcc gtaaaaaaga aaagcggctt tggctatgca aatccgggag
     5581 aactggctca ggcccaggca gattatgaag gccgcattca agaggtaaaa aacacctcgg
     5641 cagcttactt agcgaatctg gacactctaa acaccctatt gagtttgccc caaggctgtg
     5701 aagtgaaatt ctcaatttcc aatgtggttc ccacaattcc tcaactgcca gaaaagtctg
     5761 tccaagactt acgcgtgatt cgatcagaaa aattgaaagt tcaaggggcc cagagggctt
     5821 acgatgccgc caagtccaaa gatgctccga ccctgcaact gattggcaag atctatagct
     5881 acggcttaca gagtgacggc aatggcgcct atagcgacat ggtttcaggt gctcaccctc
     5941 agtactatgc gggagtgcaa tttgaatatc tgtttggcgc ggaattactc agtgaaaatc
     6001 gtctcaataa gaaatatgta aaagatcttg aagaaacaag gctttctaga atcatccaag
     6061 aatctgaaga tgtgcaagtt caagcacaga gaaaagtcca gaccacctat gctgcggttc
     6121 ttagcacaag tgagcaaaaa aaatatcgtg aaaaagccct tcaagaactc agtcgtagtt
     6181 tcaaccaggg acgtactgat atcagtatct acatcgatgc tatgaataaa ttttcaaatg
     6241 cggaagttca atacagccag gcagttggta actatcaaac agctttgaat gaatgggcgg
     6301 cggcacgaga tgaactcatc cccgacgcag ctcaggagga atgaaatctg tcttatgaca
     6361 gctgctgaaa aatatggaag agaaggacat aagctcgcaa ggtaaataat atattttcac
     6421 tttaaactag cgccattgaa aaggagctag ttatggaacc tcaacagccc gaaccacagc
     6481 gggctcccaa accaaaaata aaaatcccac tcccccaact tccggccccc aataaaacat
     6541 ccattgccaa tcaggtttgc tggtatatgg gggcttttat ggtggccctc agcatcgtag
     6601 gcgctgttat tccgggactt tttgaagcgc atttaaatcc aatccacaat tttattctat
     6661 tagcttcagg gttttcctct ctctggtttg gcctgaagac aatggattac agggctaaaa
     6721 agttttgcaa ctggatggga gggttttaca tgatgcttgg ggtagcggga tttggctttg
     6781 gccatcgagc cctttctctg acccgtccaa cagcgtcggg aattcctgaa gaaagtgcat
     6841 tcctatggca attggtccca ggaaagttcg aacttggaac cgttgatcat tttctacata
     6901 tggtgattgg agggatattt ctcttaggtg cttattttac acttaaaaaa atcagacctt
     6961 tccgtaaaaa tatttggcac tagtaaacac cggacattaa attagtagta accaactaaa
     7021 gataaagaat tgaaagctct gaattcccga gtggatttta gatcagcgga atccccactt
     7081 gattgctttt ttcactcgcc cttagccatt ttctcgagtt ccgcccaacg ggcataagaa
     7141 ctctcgatct cactttgcag ctcagccatt ttcgcgtgga gttcctgaac tcgcgaagct
     7201 ttactcacca cttccggcga agccgcttcc gcttgagtct ttgccaattc atcttccata
     7261 gcgaggattt ttggctccat accctcaagc tcattcttgt gtttgaagga catgcgtgaa
     7321 actttcgccg cgggcttttc ctccacaatt tgtggagtcg gatccactgc tgctgcggct
     7381 aactcttgat cctgtgcaaa ccaagcttct tcccattgca gataacccac aaagttttcc
     7441 agcttcttct tgggattatc ttggcgcggg aatcccagaa tatccgtcgc cacctgatcc
     7501 ataaagtaac gatcatgggt gacgattacc acggccccgt taaaattctg aatggcctcc
     7561 ccaagcacct cgagagtggg cacatccaaa tcattcgtcg gttcatcaag aatcaaaacc
     7621 gaggcatctt gcaacatcag ttgagccaga cgcagacggc tttgttcacc gccagaaagt
     7681 ctggacacag gaagatccat ctgctgacga gaaaataaaa atcgttccag ataacttctg
     7741 gcaaaaacat attgtccttg aagcgagaca taatcgccct ccggacagat gttttttaga
     7801 acactttttt ccagatccaa accggcacgg ttttgttcga aataagccac cttcacttta
     7861 tcagataaaa atatagaccc ctgatcaggt tgttctaaac ctaaaaacat tttaattaaa
     7921 gtactcttac cgctcccatt ttctccgaga agtgccaggc gggttttagg tgtgatcaaa
     7981 aaactaaaat cttggatcag atgacggtcg ccataacttt tagaaacgtt tttcaactca
     8041 attaactttt gcggatttct ttcagcatca ccaaaagcga ttcccgccac ccgcttacga
     8101 ttcttatcag tgagattctc aacatcttct ttaaggtctc cggcacgatc aatacgggct
     8161 ttttgctttg tgagacgtgc cttagctccg cgacgaagcc attcggtctc acgacgaaga
     8221 gtgttcttaa gaacggtttc caatttttgt tgcgaggcca agagctgttc tttggcttcc
     8281 acgtattgaa tatagtcgcc attcacggcc agcaagttat tcgggttgcg gggatccagg
     8341 tcaaaaacct tattcaccac ccgctgtaaa aataagcggt catgagtcac cattaaaaaa
     8401 gcaaagggcg ctctttgcag gaattcctca agccacagaa tactcacgac atcgagatga
     8461 ttcgtcggtt catccagtaa caacagctca ggctccaaaa ccaattcgcg agccaaggcc
     8521 actctctttt gccaaccccc gctcagttca gaaaccaaaa agtcctcacc aaactgagaa
     8581 agatccagac gggcaaacca ttcgtaagcc aatcccaaag cttcatcagg gtcatgggct
     8641 ctgcttaaga gagcctgcag aatcgtctcc ttcgccgcaa acacgggagt ctgtggaaga
     8701 tatcctaaac gaagtccttt tttgggagtg accttgccac catccgccgt gctaactccc
     8761 gccaaaattt tcattaatgt ggtcttacca gcgccgttcg gacctaaaag gccgatctta
     8821 tcgccatcct caatgccgag gctgacgccg gaaaaaagaa cttttccggc aaaagatttt
     8881 tcaagttggt gggtattgat taacaaggcc atgcaaacac cgaattctcg ttctcatctt
     8941 gagtgcgttt aagtttttgt agaaagtctt cgcgctcgag atattttcca ccgaaagatt
     9001 tagtgacggg tgtcaccatc tgaatgtcca tccattcgag tccccaggac tttaaaagct
     9061 gaaccaaatg aaaaagggcg agttttgaag cgttagaact cttatagaac atactctccc
     9121 cgctgaacac tccttttatg aaaacaccgt aaattccgcc tactaattgg cgctcatgcc
     9181 aaacctcgat actatgggcg taacccgcct tgtgaaaatt gatataggac ttcaccatag
     9241 agggtaaaat ccaggttccg tcctgtcccg ggcgaggttg tttagaacat tctctcacca
     9301 cctgggcaaa cgccatatcc acagtgactt caaagtcacc cgcatagtgg cgaaggaact
     9361 tttcaagact ccggctcaaa tgaaactcag aaaattccag aatccccctt ttttcaggag
     9421 aaaaccacaa cagcgg
//
LOCUS       DATBTE010000205        10842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_846479, whole genome shotgun sequence.
ACCESSION   DATBTE010000205 DATBTE010000000
VERSION     DATBTE010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 10842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10842
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846479"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VIG33_07560"
     CDS             complement(<1..966)
                     /locus_tag="VIG33_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY8270731.1"
                     /translation="MIVSQHILQKALDAALSTGADFADIFIEDTYSSQLGLLAAKPET
                     AIVGQIYGAGVRLFFGHEVVYVTTNDLSEAGLVKAALNAAQSHSHGIGKKSMPLLQVP
                     FDSVHTYGEKPWELDRNKKFKWLNSLDQHARGASSHVTQVEAMLHEKFQKVQIANSNG
                     LMAYDERAYSRVNVEVFVEKDGVKESSSEREGHMGTSEIFDTLDLKKLAEENVNRAML
                     LTTAKYAPAGEMPVVIDNSFGGVIFHEACGHGLETTSVAKGASVFCGKIGQKVANECV
                     TAIDDGTMENGWGSLNIDDEGNKTKRTVLIEKGILKNYIVDEMGSR"
     gene            complement(1285..3678)
                     /locus_tag="VIG33_07565"
     CDS             complement(1285..3678)
                     /locus_tag="VIG33_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HEY8270732.1"
                     /translation="MDQALQSYLSRIIPTVPAKSAAAVIELAAEGGTVPFIARYRKEK
                     TGNLDEVQIRAVLDGHQTYNEVVKRKAFLLKEIGDQNNLTAEIKKRIEVSWDLGELEE
                     IYKPFKKKKKTKATMAREAGLEPLANWIWELGHGLLKDEMTMEMKAKNFINPAHKIVT
                     YEEALKGAQDILVEKIANDADLRLMVNKNYMEKGRVIAKAAKGYKPHSKFEMYKEFEE
                     TVKSLFETKSSHRYMAMRRGWQEEELSVDVKGDEEELLRAYVAFATTNPDNAIGDYLA
                     QCARLALNVYVVPSVVNEVHRVLKEKSDEDAIRVFAENVRKLLLASPYGPKCILGVDP
                     GLRTGCKIALIDKGGNFISHTVLYTLGDNAEEKAKVLFKEVLKQIQIEAIAVGNGTAG
                     RETEVFLRKILKALDSHIPVVMVNESGASVYSASEVARHEFPELDVTVRGAISIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVNQPSLKKSLEAVVESCVNNVGVDLNTASTALLSF
                     VSGIGSALANNIVEYRKTKGLFTDRSELLKVPKFSTKVYEQAAGFLRIQGGKVVLDNT
                     GIHPERYTAVRDMAQELDCAILDVVGEGAKKLLTLKKKWATLIGEFTFDDIVKELEKP
                     GRDPRDPFKVFQFRDDIFEVKDLKEGMICSGIVTNVTNFGAFVDIGVHQDGLVHISAL
                     SNKFVEDPRKVVNPGDAVSVKVLKVDMEKNQISLTMRLDEAPEHKAPREMKSAMKSAQ
                     PSGARPAQQGGRQQQHVKPAQGKPLHGGGRDHRGGGKPAGNPFNNPFAALLNVTDKK"
     gene            complement(3721..3939)
                     /gene="infA"
                     /locus_tag="VIG33_07570"
     CDS             complement(3721..3939)
                     /gene="infA"
                     /locus_tag="VIG33_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEY8270733.1"
                     /translation="MAKDDLAQLDGKVTDALAGGIYKVQLDNTVEISAKLCGKMRRFN
                     IRVVVGDRVTVGVSPYDPTHGLIMYRHK"
     gene            complement(3989..4558)
                     /locus_tag="VIG33_07575"
     CDS             complement(3989..4558)
                     /locus_tag="VIG33_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270734.1"
                     /translation="MAKKTAKKEATKPAAKGKSIAKLAVKAAVKSTAKAAKPTKSVSV
                     KPAAKAVRAKPEKPAKKVAKQVKAEKPVKAPVEKPVPVVKAVPEKELKAEKPPKAEKP
                     PKVPKETKKAKAEKAAFVDGQARWGDLYQKYKSEKPQVYNMAAQFEANKPIQHKILGW
                     GFVLSNENDRLEVLFEQGIKMLISNYKTA"
     gene            4709..5770
                     /gene="lpxD"
                     /locus_tag="VIG33_07580"
     CDS             4709..5770
                     /gene="lpxD"
                     /locus_tag="VIG33_07580"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEY8270735.1"
                     /translation="MVTAAKIHELQSGSSAEVLHYVQGPLSAQVSKVLPPESANSECL
                     AFVSKQEQLNLALKNKTSIIIAHNSLAIPEKSQACFFTTPQIQLAMALVGKLFDKKSQ
                     RFQQEEHIHPKSCIHPSAQLGKNVIVGPGAVIGAEVHIGDNSIIGANTVIETHAQIGR
                     NTMIHPLVFIGAYCEIGAFCEIQPHVTIGADGFGFVPLKEAHPVKIPQNGRVIIEDHV
                     EIGANCTIDRAALTETRIRAGAKFDNLIHIAHNCEIGPNSLIAAGFMMAGSSKIGKNF
                     MTGGRVVVADHVDITDNVMIAGLSAVTNDISEAGQYGGYPLQSLKDSLKTLANTVHLT
                     KMRKQIGRIVKHLNLSEEK"
     gene            5795..6160
                     /locus_tag="VIG33_07585"
     CDS             5795..6160
                     /locus_tag="VIG33_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270736.1"
                     /translation="MSYEFYKILHLIGLMLLFFGLSAALTLKMAGAAFTGSVKKMAFL
                     THGVGLFLMLLGGFGLLAKLGFMGNIPAWAWVKLGIWALLGVAISLAKRKGQLGWPLM
                     VLFIGLGTTAAWLAIAKPF"
     gene            complement(6157..7395)
                     /locus_tag="VIG33_07590"
     CDS             complement(6157..7395)
                     /locus_tag="VIG33_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270737.1"
                     /translation="MDFKKTLVIFGAIYCAVMAGCGVNSLSDKTSDNIIGNPTQSVTT
                     LSTPDEGESQNLVIFDKTVRRIHQFDLTSMKHVRSFAVRNPEEEHFVLYGQNGNYIVD
                     LSFKGLGIFDRSNQVNHQPIKFQGVPKSAAFLPSKGLLIVYDDLMSVGMLKLDINGEV
                     LGSWVGGASIVGTNSIAAGDLNEEGKLILALSDGSVIVVDPEQSMTKKSWMIESVFNT
                     ELSNIKWVAPLPKTPNQILIRSSSKLSLLELNSKTIISSYDIFDEVVKLSKFNDPHIV
                     MNNGNEVKVAYVEKSQIKVKTFYLHKMKSFEMNYLMSSNLDLVKDTWSFVDTKEVVNY
                     FFNDLDQSKKDRRFVRYRFSDALAIHNMPVANDTQVEIADSFIFALFPRELGYAVRYE
                     VETGNASPLERFNLKYIPVD"
     gene            complement(7385..8581)
                     /locus_tag="VIG33_07595"
     CDS             complement(7385..8581)
                     /locus_tag="VIG33_07595"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF039181.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative metallopeptidase"
                     /protein_id="HEY8270738.1"
                     /translation="MRNFIFLFTVLFFQQSTASTFVGNGGNAGDIELQMTLGQIQKSL
                     DYLDRDKDAEAQNLCVCSKSFEGRPLCDVLNHLNSEQVSFCSKFIIQKAPEIARILTQ
                     KRELSFSWTHQEIEVQEEGKLRGVDAVTNAKNRSITLNQKRFLEMDENEKIFLVGHEL
                     FHLTAFEGKTLEDEGEIGPFKGVDGGRHFINALAATVVLQANEYGVFRAYEEAERRSK
                     SYKKNWITLGMGSVSTANNPNTAFDIEKASGGQVGYRYQWTQEIGLLAHFSALKGTKK
                     ILSTIEAAENRNIFGLGASYRWFPFSNPLTASGQSHFIFGGTIDLLDGTYKFHDLNPG
                     TTSKTSSVGYALTCNYFIPFNSGVWAYGGMGYSNLHYSFNLDNQMDLNYENNGTTFAL
                     GVSYGF"
     gene            complement(8578..9420)
                     /locus_tag="VIG33_07600"
     CDS             complement(8578..9420)
                     /locus_tag="VIG33_07600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HEY8270739.1"
                     /translation="MDIEFAEKPLLRPNIVNYEDSVDFLKAMIHFRKKTEKTFSVHAA
                     TAPLRKISPTLISLILVRKRKITLDRVEELAKLLQLNVQEKIYFKNWVEREASGENKV
                     LTAPKTDQQARHQRKEVGVPILNDWINVYVKDCFQLPKVQSRPELIYRYLSSYAHPKR
                     IEKSINFLLKEGHLRRTLEGKIVVEANLAVADPQVPNKKIRQFHKGALGVAKNALDLF
                     PPDERLANTLIIALNEKSYGELLEMIHEFSERLQSFATHNQEAGERLYQLILNLSPAG
                     GKVE"
     gene            complement(9556..10116)
                     /locus_tag="VIG33_07605"
     CDS             complement(9556..10116)
                     /locus_tag="VIG33_07605"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY8270740.1"
                     /translation="MRKTKRLILRPLELHDYENWRQAYSILHTAQNEWDEGPWKESEL
                     TLAKFKKLLKVRKDLSKKDQFYDFAIFRKDDGVFVGLVGLMNVSRMIFQNAYLGYRIF
                     NNYWGEGYASEACRAGIEIAFKDLKLHRIEAGIEPHNRRSIRVAKAIGLRKEGLSSKR
                     LLFQGKWKDMLIYAGTCEDFKMKYRK"
     gene            complement(10162..>10842)
                     /locus_tag="VIG33_07610"
     CDS             complement(10162..>10842)
                     /locus_tag="VIG33_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY8270741.1"
                     /translation="LVAIDLSGDSLFMRGYRKEVGEAPLKENLAAGLLMLTEWDRQSS
                     LLDFMMGSGTFLIEGAMMALKIAPGLQRKGFGFQRWLNYDSKAWDKLVDEAISQEVEE
                     LPFKFYGYDIDRRMIVAAKENAKSAGVDHVMEFKREPVATVTPPAEKGLAIVNPPYGA
                     RIGDEDNLRDVYRDLGFTLKHRFKGWDCWILSGNKELIADLKLKSTRKYFVFNGNIEC
                     RWLKYSMF"
BASE COUNT         3078 a         2609 c         1968 g         3187 t
ORIGIN      
        1 acgagagccc atttcatcga cgatgtagtt cttcaaaatt cccttttcaa ttaaaaccgt
       61 gcgctttgtt ttatttcctt catcatcgat atttaaagaa ccccaaccgt tttccatagt
      121 cccgtcatca atagcagtca cgcattcatt agcgactttc tggcctattt tgccacaaaa
      181 aacggaagcc ccttttgcca ctgaggtggt ctcaagaccg tgaccgcaag cctcgtggaa
      241 aatgacccca ccaaaagaat tgtcaataac cactggcatc tcgccagcgg gcgcatattt
      301 tgccgtggtc aaaagcatcg cgcggtttac attttcttca gctaactttt taagatccag
      361 agtatcaaaa atctccgaag ttcccatatg gccttcccgt tcactcgagc tttctttgac
      421 tccgtctttt tccacaaaga cttcaacatt cactcgcgag taagcccgtt catcataagc
      481 cataagacca ttcgagtttg caatctgcac cttttgaaat ttctcgtgaa gcatcgcttc
      541 gacttgggtc acatgggagc ttgctcctcg agcatgttga tctaaagagt tcagccattt
      601 aaactttttg ttacgatcca gctcccaggg tttttcgcca taggtatgaa ccgagtcaaa
      661 aggaacctgt aaaagtggca tcgatttttt tccgatgcca tgactatggc tttgagccgc
      721 attcaaagcc gcctttacta gaccagcttc actcaaatca tttgttgtca cgtagactac
      781 ttcgtgacca aaaaataaac gcactcctgc accataaatt tgaccgacaa tggccgtttc
      841 tggcttcgct gctaaaagac ctaactgaga agaataagta tcctcgataa agatatctgc
      901 aaaatcagct cctgtagaaa gagccgcatc cagagccttc tgtaaaatgt gttgggacac
      961 aatcatttaa acctccaaaa attaattgta tctaaggaaa gacttgaggc caagctttct
     1021 tcagtttcgg acgaatcatc ccctatttat ccggtattta tcttgataca gtcattatcg
     1081 aaagagaact ctctatttct tctgagccta aaaaacaaaa aaccttcgga gacccagaag
     1141 gttttttgtt tttatatgtt aagaagaaaa ctatttacgg agcttattta cgaaacttcg
     1201 ctttagtcat tctcttcttt ttactaagtc tttttgcttt aagaagagat ctttttttag
     1261 tccttttatt agccaagagg ccccctattt cttatcggtt acatttaata atgctgcaaa
     1321 tggattattg aacggattcc ccgctggctt accgccaccg cgatgatccc gccccccccc
     1381 atgaagaggt ttgccctgtg caggtttaac atgttgctgt tgccttccac cttgctgcgc
     1441 aggtcttgca ccgctcggct gagctgattt cattgctgat ttcatctccc gaggagcctt
     1501 gtgctccgga gcctcatcca atctcatggt taatgaaatc tgatttttct ccatatccac
     1561 tttcaaaact ttcacgctca cagcatcgcc tggattcacc actttacgcg gatcctccac
     1621 gaacttatta gataaagcac tgatatgaac caggccatct tgatgaacgc cgatatcaac
     1681 aaaagcacca aagtttgtca cattggtcac gatcccggaa caaatcatgc cctctttaag
     1741 atctttcacc tcgaagatat catcacgaaa ttgaaatacc ttgaagggat cacgaggatc
     1801 tcgtcccggt ttttcaagtt ctttaacaat gtcatcaaaa gtgaattccc ctatcaaagt
     1861 ggcccacttc tttttcaaag tcagaagctt tttagctcct tccccaacca catctagaat
     1921 cgcgcaatcc aattcctgag ccatatcccg aaccgcagta tagcgctctg gatgaattcc
     1981 cgtattatca agaacaacct taccaccctg aattctcaag aatcctgccg cttgctcata
     2041 aactttggtg gaaaatttag gcactttcaa aagttccgaa cggtcagtaa atagaccctt
     2101 ggtttttcta tattccacga tattgttcgc tagagccgat ccaatacctg atacgaaaga
     2161 aagcaaagcc gttgaagccg tatttaaatc cacacctacg ttattcacgc aggactcaac
     2221 cacggcctcg agagacttct tcaatgaggg ttggtttaca tcatgctgat attgccccac
     2281 accaatagat tttggatcga ttttcacaag ttccgccaat ggatcttgca aacgacgggc
     2341 aatcgaaatc gccccacgca cagtcacatc caattctggg aactcatgcc gagccacttc
     2401 ggaagccgaa taaacactag caccagattc attcaccatt accactggaa tatgagaatc
     2461 gagagctttc aaaattttcc tcaagaaaac ttcggtctca cgccccgctg ttccattgcc
     2521 gacggcaatg gcctcaattt gaatctgttt taacacttct ttgaaaagaa ccttggcctt
     2581 ttcttcggcg ttgtccccca aagtataaag gactgtatgg gaaataaagt tgccgccttt
     2641 atcaattaag gcaatcttac aacctgttct taaacctgga tctacgccta aaatacattt
     2701 tggtccataa ggagaagcca acaaaagttt acgcacattc tctgcgaaaa ctcgaattgc
     2761 atcttcatca cttttttctt tcaaaacacg atgaacttca tttactacag aaggcactac
     2821 ataaacatta agagccaagc gcgcgcattg agctaaataa tcgccaattg cattgtctgg
     2881 attggtcgtc gcaaaggcca cataagctct gaggagctct tcctcatcac cctttacgtc
     2941 aacagagagt tcttcctcct gccagccgcg tcgcatcgcc atataacgat gtgaactctt
     3001 agtttcaaaa agacttttta cagtctcttc gaattctttg tacatttcga acttggagtg
     3061 aggtttatag cctttagccg ctttcgcaat cactcggcct ttttccatat agttcttatt
     3121 taccatcaaa cgaagatctg catcattggc gattttctcg accaagatgt cttgagcacc
     3181 tttaagagct tcttcatagg tcacaatttt gtgagccggg ttgatgaagt ttttagcctt
     3241 catctccatg gtcatttcat ctttgagaag accgtggcca agttcccaaa tccaatttgc
     3301 caaaggctca agtcccgctt cacgagccat tgtagctttg gtttttttct ttttcttgaa
     3361 gggcttataa atctcttcaa gctctcccag atcccatgaa acctcgatac gtttcttgat
     3421 ttcggcagtg agattatttt ggtcgccgat ttctttcagc aagaaggctt tgcgcttcac
     3481 gacttcattg taagtctgat gaccgtcaag tactgcacgg atttgaacct catcgagatt
     3541 cccggttttt tccttacggt aacgagcaat aaaagggacc gtccctccct cagcggccaa
     3601 ttcaataact gcagctgcgg actttgctgg gacagtggga ataatacgag agagataact
     3661 ctgaagagcc tgatccataa tacctctatt ttgatgaact aataggagag atcaaatgaa
     3721 ttatttgtga cgatacatta taaggccatg agtcggatca tagggggaaa ctccaacggt
     3781 cacccgatca cccaccacaa ctcgaatgtt gaatcggcgc attttaccgc acaactttgc
     3841 agaaatctct actgtgttgt ccagttgaac cttatagatg cccccagcga gagcatctgt
     3901 aaccttacca tctaattgag ctaaatcgtc ttttgccatg cctctcaata tgtaggtgag
     3961 ttttagaagc taagcaataa atttctcact aagcggtctt atagttacta attagcatct
     4021 taattccctg ctcaaataag acttctaatc ggtcattttc attcgacaac acaaaccccc
     4081 aacctaaaat cttatgttgt attggtttat tggcctcaaa ctgagcagcc atgttataaa
     4141 cttgaggttt ttcagattta tatttctgat aaagatctcc ccaacgggct tgaccatcca
     4201 caaaggcggc tttctcagct tttgctttct tggtttcttt gggcaccttc gggggttttt
     4261 cagcttttgg tggcttttcg gcttttagct ccttctcggg aactgcctta acaacaggaa
     4321 caggcttttc gacgggagct tttaccggct tctcagcctt tacttgcttt gccacttttt
     4381 ttgctggctt ctccggcttt gccctaactg cctttgccgc aggcttaact gagactgact
     4441 ttgtgggttt ggcagcctta gctgtggact tcactgctgc tttaacagca agcttcgcaa
     4501 tagatttccc ttttgcagca ggctttgtgg cttctttttt ggcagttttc ttcgccatcg
     4561 acaatgtcct ccccttattt caagaagatc tacgaatggt caaaacactt taacctatta
     4621 atataagaga tttatactat tacccgtagt cttgacagca aaaaaataat aggattctct
     4681 tatgcaaagc atcaccaaag aggtgtttat ggttacagca gcaaaaattc acgaactcca
     4741 aagcggatct tctgcagaag ttctgcacta cgtccaagga cctttaagcg cacaagtttc
     4801 gaaggtgctt ccacctgaaa gcgctaactc cgaatgtctt gctttcgtca gtaagcaaga
     4861 gcaactgaac ctcgccctca aaaataagac ttcaattatt attgcccaca atagccttgc
     4921 tattcctgaa aaatctcaag cgtgtttctt tacaactccg cagatacaac ttgccatggc
     4981 tctcgttgga aaactgttcg ataaaaaaag ccaacgcttc caacaagagg agcacatcca
     5041 tccaaaaagc tgtatccacc cttcagctca gttaggaaag aatgttattg tgggacctgg
     5101 cgcagttatc ggcgccgaag ttcacatcgg cgataacagt attatcggag ccaatacagt
     5161 tatcgagacc catgcacaaa ttggtcgaaa caccatgatt catccgctag ttttcatagg
     5221 agcatattgt gaaatcggtg ccttctgcga aattcaaccg catgtaacca tcggtgcaga
     5281 tggctttggt tttgttcctc tcaaagaagc tcatcccgta aaaattccac aaaatggaag
     5341 agtcatcatt gaggaccatg ttgaaattgg tgcgaactgc acgatcgatc gcgctgccct
     5401 caccgaaacg cgcattcgcg ctggagcaaa atttgacaat cttattcata ttgctcataa
     5461 ttgcgaaatt ggacccaact ctttgattgc agcaggtttt atgatggcgg gttccagtaa
     5521 aatcggtaaa aactttatga ctggcggaag ggtcgttgtc gctgatcacg tggacattac
     5581 cgataatgtc atgattgcgg gactttcggc cgttaccaat gatattagcg aggctggtca
     5641 atacggaggc tatcctttac aatccttaaa agattctcta aaaactttag ccaacacggt
     5701 tcatctgacc aaaatgcgca agcagatagg acgcatcgtg aaacatctga atctttccga
     5761 ggaaaaataa ttccaaggaa aaaaggagtc ccccatgtca tatgagttct ataaaattct
     5821 tcacctcata ggtcttatgc ttttattttt tggcttgagc gccgctttga ccttaaaaat
     5881 ggctggagcc gcgttcacag gctcagtaaa gaagatggct tttctcactc acggtgtggg
     5941 gcttttttta atgcttttgg gaggctttgg tcttcttgcg aaattgggct ttatgggcaa
     6001 cattccagca tgggcctggg taaaactcgg gatttgggct ctcctaggtg ttgccatctc
     6061 tttagcgaaa agaaaaggcc agctaggctg gcctttgatg gttcttttta tcggtttagg
     6121 cacaactgcc gcttggctag cgattgctaa acctttctag tccacaggaa tatatttaag
     6181 attgaaccgc tccagtggag acgcgtttcc cgtctccacc tcataacgaa cagcatatcc
     6241 cagttctcgt ggaaaaagag cgaaaataaa actgtcagcg atttccacct gagtatcatt
     6301 tgccactggc atattgtgaa tagctaaagc atcgctaaac cgataacgaa caaatcttcg
     6361 gtccttcttc gattgatcta aatcattaaa gaaataattc accacttcct tagtatccac
     6421 gaaagaccaa gtgtccttta ccaagtccaa attcgaactc attaagtagt tcatctcaaa
     6481 gcttttcatc ttatgaaggt aaaaagtctt tactttgatt tgagactttt caacataagc
     6541 gactttgact tcattcccat tgttcataac aatgtgcgga tcattaaatt tagaaagttt
     6601 cacaacttca tcgaagatat cataactcga aataattgtt tttgagttca gttccaacaa
     6661 agaaagctta ctactggaac gaatcagaat ttggttcggg gttttcggaa gcggtgccac
     6721 ccattttata ttgctcagct cagtattaaa tacagactca atcatccaac tctttttagt
     6781 catactctgc tcagggtcca caacgatcac cgacccatca ctcaaagcta aaatcagttt
     6841 cccctcttcg ttcaaatcac cggcagcaat agaattagta ccaacgatgg aggcccctcc
     6901 gacccaagag cctaaaactt ctccattaat gtcaagtttc aacattccta ctgacatcag
     6961 atcatcataa acaatcaata aacctttgct cggtaagaag gcggctgact tcggcactcc
     7021 ttgaaattta attggttgat gattaacctg gttgcttcta tcaaaaatgc caagcccctt
     7081 gaaagacaaa tccacaatat agttgccatt ttgaccatat aaaacaaaat gttcctcttc
     7141 aggattacga actgcaaaag atctcacgtg tttcatactc gttaaatcaa attgatggat
     7201 gcgacgaaca gtcttatcaa aaatgaccag gttctgagat tccccttcgt caggtgtgct
     7261 taaggttgtc acactctggg taggattccc gataatatta tcggaagttt tatcacttaa
     7321 agagttcact ccacaaccag ccattacagc gcaatagata gccccaaaga taactaatgt
     7381 ttttttaaaa tccataactc actcctaaag caaaggtcgt tccattattt tcgtaattaa
     7441 ggtccatctg attatccaga ttgaaactat aatgcagatt tgaataaccc attccaccgt
     7501 aagcccaaac gccagaatta aacggaataa aataattgca agtcagtgca taacccacag
     7561 atgaagtttt tgaagttgtg cctgggttaa gatcatgaaa tttataggtc ccatcaagaa
     7621 gatcaatagt tcccccaaaa ataaagtgcg attgccccga cgctgttaag gggttggaaa
     7681 aaggaaacca acgataagag gcgcccaaac caaaaatatt tctattttcc gcagcctcaa
     7741 tggtcgaaag aattttttta gtgcctttca atgcggaaaa gtgtgccaac agcccaatct
     7801 cttgagtcca ttgataacga tatcccacct gcccaccgct ggctttctca atatcaaaag
     7861 cagtgttagg attattggcc gtcgacacgg accccattcc tagggtgatc caatttttct
     7921 tatagctctt agatcgtctc tcggcctctt cataggccct aaacacaccg tattcattgg
     7981 cctgcaaaac caccgttgcc gccagggcat tgataaaatg acgtccacca tccactcctt
     8041 taaagggacc gatttctccc tcatcctcta aggtctttcc ttcaaaagca gttaaatgaa
     8101 aaagttcgtg cccaactaaa aaaatctttt cgttttcatc catctcaaga aaacgttttt
     8161 gattgagagt tatggaccta tttttcgcat tggtcacagc atctacaccc cgaagttttc
     8221 cttcttcttg aacttcaatt tcttgatgag tccacgaaaa acttaattcc ctcttctgag
     8281 ttaatatccg agcgatttcc ggcgcctttt gtattatgaa ctttgagcaa aaactcacct
     8341 gctccgaatt cagatgattc aagacgtcac agagaggacg gccctcaaaa ctcttggaac
     8401 acacgcaaag attttgcgcc tctgcatctt tatcgcgatc aaggtaatca agacttttct
     8461 ggatctgccc aagagtcatt tgtaattcga tgtctccagc gtttccaccg tttccaacga
     8521 atgttgaagc ggtgctctgc tgaaaaaata atactgtgaa taagaagatg aaattcctca
     8581 ctcgaccttc cctcctgccg ggcttaaatt caaaataagc tgataaagac gttctccggc
     8641 ctcttgatta tgagtggcaa aactctgcaa tctttctgaa aactcatgaa tcatctctag
     8701 aagctcaccg taactttttt cgttcaatgc aataattaat gtattggcaa gacgctcatc
     8761 gggcggaaac aaatccagag cgttctttgc aacacccaaa gctcctttat gaaactgtct
     8821 tattttttta tttggcactt gaggatctgc cactgcaaga tttgcctcga ccactatctt
     8881 accctctaag gtgcggcgca aatgcccttc cttcaaaaga aaattaatag atttttcgat
     8941 acgtttcgga tgagcataag aagataagta acgatatatc agctcaggac ggctctgcac
     9001 ttttggcaat tgaaagcagt ctttgacgta aacattgatc cagtcattca ggatagggac
     9061 tcctacctct ttacgttgat gtcgggcttg ctgatctgtc ttaggcgcag tgaggacttt
     9121 attttcgcca gacgcctcac gttcaaccca gtttttaaaa taaatttttt cctgaacatt
     9181 gagctgaaga agtttcgcaa gttcttcaac ccgatcaaga gtaatttttc ttttccgtac
     9241 cagaattaac gaaatcagcg ttggtgaaat ttttcgtaag ggagctgtgg ctgcatgaac
     9301 tgaaaaggtc ttctccgtct tctttcgaaa gtggatcatc gccttcaaaa aatccaccga
     9361 atcttcgtaa ttaacgatgt taggccgtaa taagggtttt tcagcaaact caatatccat
     9421 aactattgat gaagatattt taaatatctt tcaatttcaa tatgttatgg aataacaaat
     9481 tttactttgt aaaaataatt aaaaatgcaa taaaaaaccc ctatttggag ttcgccttgc
     9541 aattgaaaat ttaagtcatt ttctatattt cattttaaaa tcttcacagg tgcctgcgta
     9601 aataagcata tctttccact ttccctgaaa taataaacgc ttcgagctca acccttcttt
     9661 gcgaagccca atcgcctttg ccacccgaat agaacgccta ttatgaggtt caatccccgc
     9721 ttcgatgcga tgaagcttca aatctttgaa agcaatctca atccccgcgc gacaagcctc
     9781 agaagcatat ccttcacccc agtaattatt aaaaatacga taacccaaat aggcattttg
     9841 aaaaatcata cgcgaaacat tcattaatcc taccaaacca acaaagactc catcatcttt
     9901 acggaaaatt gcaaaatcat agaattgatc tttcttgctt aagtccttgc ggacttttag
     9961 taatttttta aactttgcaa gagtcaactc actctctttc caaggccctt catcccattc
    10021 attttgagca gtatgaagta tagagtaagc ttgtcgccaa ttttcgtaat catggagttc
    10081 taaaggtcta agaataagtc tcttggtttt acgcatgtct atattacctc atggatttcc
    10141 atatgcgatg gacagaaatt tttagaacat agaatacttc aaccagcggc attcgatatt
    10201 accattgaaa acaaagtatt ttcgagtaga ttttaacttc aaatcagcaa tcaattcttt
    10261 attgcctgaa agaatccagc aatcccaacc tttaaaacga tgtttcaagg tgaaaccaag
    10321 gtcacgatag acgtcacgca gattgtcctc atctccgata cgagctccat aaggcggatt
    10381 gacaatcgcc agaccctttt ctgccggagg agtgactgtt gccacaggct cacgtttaaa
    10441 ttccatgaca tgatccacac ctgcactctt agcattttct ttagcggcaa cgatcatacg
    10501 gcggtcgata tcatacccat agaatttaaa aggcaactct tcgacttctt ggcttatggc
    10561 ttcatcaacc aatttatccc aagccttcga gtcataattc aaccaacgtt ggaaaccgaa
    10621 acctttacgt tgaagtcccg gcgcaatttt caaagccatc attgcccctt ctattaagaa
    10681 ggttccggaa cccatcataa aatccaggag agaactttga cgatcccatt ctgtcagcat
    10741 caaaaggcct gccgccaggt tctcttttaa gggagcttct cccacttcct tacggtagcc
    10801 acgcataaat aaactatccc cggaaagatc aatagcaacc ag
//
LOCUS       DATBTE010000206         1733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_914758, whole genome shotgun sequence.
ACCESSION   DATBTE010000206 DATBTE010000000
VERSION     DATBTE010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914758"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="VIG33_07615"
     CDS             complement(<1..924)
                     /locus_tag="VIG33_07615"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8270742.1"
                     /translation="MSLAPALLVALEKMSITKPTAIQRQAIPVGLNGSDLIAIAQTGS
                     GKTLAFALSTLTRLLNKPESRALILVPSREMAQQIYKVFLELCSELPISVCLAVGGTT
                     GSKQANQLKKNPRLIVATPGRMNDHLMGNKLLLQNVSIVVIDEADRMLDMGFAPQLQN
                     IQSTLRGPRQTLMFSASFGQNVESIAQLFMNEKAFMIRSILAEEPVTSLKQKVLFLDR
                     TMKQDRLLDELNATKGGVIVFTGSQESCENVGEYLKEYGFSTDLIHGGFSQGQRNRVV
                     REFREGTIRIVVATDLLARGIDVPHVAHVINF"
     gene            1183..>1733
                     /locus_tag="VIG33_07620"
     CDS             1183..>1733
                     /locus_tag="VIG33_07620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HEY8270743.1"
                     /translation="MRHFEKRIPLLLELGFRKEALPQLKSYIDLLWTANEELNLISRK
                     MTYEELIDNHLIDCLLPLKKFPTGIKSAADFGSGGGLPGVVYAIQFPNIAFHLFEKSK
                     MKQEFLTRCKAMAPNLQIHGDIPLELKNIEIVTARAFKPIEVILDVSRRYYEKGGKYF
                     LLKARREKIDEEVALARKKFKQL"
BASE COUNT          516 a          405 c          376 g          436 t
ORIGIN      
        1 aaagttaatg acatgggcca cgtgaggcac atcaataccg cgcgctagca agtcagtggc
       61 cacgacgata cgaatagtcc cttcgcgaaa ttctctcacc acacgattcc gttgcccctg
      121 agaaaaacct ccatgaataa gatccgtaga aaatccatac tcttttaaat attcgcccac
      181 attctcgcaa ctttcttggc tcccagtaaa gacaatcact ccccctttag tggcattgag
      241 ctcatccaag aggcgatcct gcttcatcgt tctatctaag aacaagactt tctgctttaa
      301 actggtgacc ggctcttccg caaggatcga gcgaatcatg aaagctttct cattcataaa
      361 aagctgtgcg atggactcca cattttgacc aaaactcgca gaaaacatca gagtttggcg
      421 gggaccacgc aaagtgcttt ggatattctg aagctgggga gcaaacccca tatccaacat
      481 acgatcagct tcatcgataa ctacgatact tacattctgg agaagcaatt tattacccat
      541 caaatgatcg ttcatgcgtc ctggggtcgc cacaatcaaa cgcgggttct ttttaagctg
      601 attggcttgc ttagaacccg tggttccacc gacggcaaga caaaccgaaa taggcaattc
      661 agagcaaagc tccaaaaata ctttataaat ttgctgggcc atctcacgac tgggcactaa
      721 aatcaaagct cgggactcag gcttattgag aagacgagtc aatgttgata aagcaaaagc
      781 caaagtcttt ccactgccgg tctgggcgat ggcaatcaaa tctgaaccgt tgagaccgac
      841 gggaatggct tgtctctgaa tagccgtggg tttagtaatg gacatttttt caagagctac
      901 cagcaaagct ggggcaaggg acatttcggt aaaactctta agatgaagag gagaggtttg
      961 gaatttcata gaaggagctt taccacagac tccaaggatg acaaaggatc aattctgagg
     1021 cagactcccc caaaacggag agccccatgt cagccaatgt tcccccgcag ccttctaagt
     1081 ccgcccgtat cgtcctagtt ttgccaagat ccgagaaaag gctggataac gtcctcttaa
     1141 aagccattag agagcaacta tgtagatatt ttagggtttt agatgagaca ctttgaaaaa
     1201 agaattcccc tcctcttaga acttggcttt cgtaaagaag ctttgccaca attaaaatcc
     1261 tatattgatt tgctttggac agcgaatgag gagctcaatc taataagccg taagatgact
     1321 tacgaagaac tgatcgacaa tcatctgatt gattgccttt tgcctcttaa aaagtttcca
     1381 acgggaataa aaagtgccgc tgactttggc tcaggcggag gccttcctgg agtggtctat
     1441 gccatacaat ttccgaatat tgcctttcac ctgtttgaga aaagtaaaat gaaacaggaa
     1501 tttctaactc gttgtaaagc gatggcaccc aacttgcaaa ttcatgggga tattcccctg
     1561 gagttgaaaa atattgagat cgtcaccgct cgcgccttta aacctattga ggttattctc
     1621 gacgtgagcc gccgctacta cgaaaagggt ggaaaatatt ttcttctaaa agcccgtcgc
     1681 gagaagatcg acgaggaggt ggctcttgca cggaagaaat ttaagcaact tca
//
LOCUS       DATBTE010000207         8704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1802850, whole genome shotgun sequence.
ACCESSION   DATBTE010000207 DATBTE010000000
VERSION     DATBTE010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8704
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802850"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VIG33_07625"
     CDS             complement(<1..193)
                     /locus_tag="VIG33_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270744.1"
                     /translation="MKVDLDSPYIAYKVDPMFATNARDGYVLAGGGAARVSYSAQISF
                     ECAKELRALAELFIHLAGAE"
     gene            complement(220..750)
                     /locus_tag="VIG33_07630"
     CDS             complement(220..750)
                     /locus_tag="VIG33_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270745.1"
                     /translation="MELEWQEERLDYLYKKAEKQAVTVELLKQRIIEQKKHERDHLQV
                     IGELGELTSDQKKAIKRNPIRGDDMDSWRRRIEKIRTTYDRNVRIEVTFSPNEKKYHA
                     RIFKGRHLSETVTLPAWNKIGIKRLIDRAQEIKVPWKQIEYGQPWIADIKLNPYYIEK
                     NWVPQNVIPFTQRLTK"
     gene            complement(941..1468)
                     /locus_tag="VIG33_07635"
     CDS             complement(941..1468)
                     /locus_tag="VIG33_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270746.1"
                     /translation="MENQQSEFEMRSSPVELVKSEPSPYNLAEATIGQRQIKQVKPER
                     TGWRDGHLSERHRKWGLALPAVDLDFLLIEYDKGRPSALIEYKSEFATAQFPSHPSYL
                     ALSQLGERAGVPVFAVRYGQTFTWWKVTALNSVAKRMHPTRLEMSEREFVTWLYEIRG
                     LKPPAEIFEWLETAI"
     gene            complement(1449..1742)
                     /locus_tag="VIG33_07640"
     CDS             complement(1449..1742)
                     /locus_tag="VIG33_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270747.1"
                     /translation="MARLFGRDEKVKSTLRIISGGKDIPPPARDPVEFFEHELLQLTP
                     DEAVKVWNIDFISEKFRELKFVSKNLGVDCRNKSIGKIIGEIKEKQRHGKSAI"
     gene            2058..3488
                     /locus_tag="VIG33_07645"
     CDS             2058..3488
                     /locus_tag="VIG33_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270748.1"
                     /translation="MPYAAPLVDDENDPNKKSFGNPVNISGQSTTISTGIPGQDASSV
                     PGVKQKTSGNYTNIQSYLDANKEQGDTMGAKIAGDVTSKADDATSKIGAYEAKAPSVQ
                     AYDPNEAISRATSLSDSEKAQYKTNKATGGYSGPQTADGIEGYQDANKAGTEAVAAIK
                     NTGTERGQRELLKQTYNRPQYTAGENNLDQTLVQNSAGSKSRLEQIGPKYSKLSDYLS
                     GANAKVGNAVNAANSQAFANKQAFIPAEQSARTALVSPIQARANQANQNNPAYINRVL
                     DDASDNVISEETLKALGLNEGQDLFDMKLRDYVNQDWSPVGIDNAATNDERNKYAALN
                     ALFDDPTMNQLTVDGKAIEPVKLNKEKFDKDLAAKKAEYNDAYTNQRGTIFNEKYMPY
                     GKGDVNQWIPGALTDRRDLSTATASELENYWFPLLDQAHQQAAAAGQAGAPGGNSVYS
                     WAKNAMQQSLNDWKNAYQVNRKINKG"
     gene            complement(3562..3912)
                     /locus_tag="VIG33_07650"
     CDS             complement(3562..3912)
                     /locus_tag="VIG33_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270749.1"
                     /translation="MFAKGMKKVEGSGRKPGTPNKKSLLRVEEVLAEKNVNPTEEILA
                     IIPSLDPNEQVRAWGMLLSYIQPKPASGGVGKSDSSDDTNILTIEDVLEMTAHIPNEF
                     LIEMAKKKDHEEKA"
     gene            complement(3955..4380)
                     /locus_tag="VIG33_07655"
     CDS             complement(3955..4380)
                     /locus_tag="VIG33_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270750.1"
                     /translation="MLWQPCNEKNMIDKESGLYKLELILIGLPQRINEAPGASWHTRY
                     KESQRWHKRVLGRMLIGRLSPPLLPLEKAHLTLIRHSRRAPDYDGLVHSFKPVIDALK
                     KCLIIKDDNMRIIGRPDYRWEKAAPKDGKIEVIVREVKA"
     gene            complement(4361..4894)
                     /locus_tag="VIG33_07660"
     CDS             complement(4361..4894)
                     /locus_tag="VIG33_07660"
                     /inference="COORDINATES: protein motif:HMM:NF036900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270751.1"
                     /translation="MSPAQLISSLIKKGVQVSYKGDALVYWPSQLVTGRDREEIKERK
                     AHLIHYLRGVYQLKRGDPVTNASGNKNVWEIYPESNRLGLIDNEGAKEFCSYDEIASH
                     SQFQFLKSRISLIETYVYAQPVAKRAEAFACFKHEWNFLFKELNRQQFLIETYCGPIG
                     GSKTNQGKEIQNVMAAM"
     gene            complement(4891..5778)
                     /locus_tag="VIG33_07665"
     CDS             complement(4891..5778)
                     /locus_tag="VIG33_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8270752.1"
                     /translation="MKKGKIVLFSSEEDPSDTLRPKCERHGADLSQIYVYPEPFELNS
                     DGLVTVEEVLSSLSPVALVVFDTLQNYFGGKDMNKVNEVAEVMTAIQSMAKRFSCAVI
                     CVRHLKKGADKANYRGMGSIGITGAARSEVLLGSNPKNTAELAMIHTKSNCAPYGQSL
                     GFKIEKGVLIPTGPTALTADDLMALNLSGSEERSALEEAVEFLIESLRYGKVPAKKIQ
                     REANEVGISERTLKRAKKEIKVECNKVGDQWFWSIPSGPKNEEGQLSHVAPLAPFPVL
                     APLNGSDDENQGIFNMGTV"
     gene            complement(5765..6517)
                     /locus_tag="VIG33_07670"
     CDS             complement(5765..6517)
                     /locus_tag="VIG33_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8270753.1"
                     /translation="MAIETIAIYQDFNGNDIQKVIRKDNPGGKKSFSQWSLENESWIP
                     KAPKGEVAPLFYREWKDKPNVILGEGEKVAQRLKELGLNATATPGGAGAWKKHYAKAF
                     AGKVVSILPDNDEAGRKYASHAFRDIKEVAKSTRIVDLPGLKGKEDVVDWLDAGGTKE
                     QLLAIIAEAFDVESHDVLASRERLKTKLICLNDVQSTQVEFLVEPYFPIGKVSLMSGD
                     PGVGKSTFVAMLASAISAGRSISLAGSDNEKG"
     gene            complement(7062..7292)
                     /locus_tag="VIG33_07675"
     CDS             complement(7062..7292)
                     /locus_tag="VIG33_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270754.1"
                     /translation="MSLPHSESHEYQNALVSARTDSRKLFRTLEAITQALPNRDVSDK
                     ADPFKILHRGLLKAIDELYLEIEEMKGETWPS"
     gene            complement(7477..7692)
                     /locus_tag="VIG33_07680"
     CDS             complement(7477..7692)
                     /locus_tag="VIG33_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270755.1"
                     /translation="MNTNLNDLQEHISTKEVAKRTGTSSRFWDGYRSKGGGPNFVKIS
                     ARCVRYRWGDVLDWLASRKSDSRGGAL"
     gene            7801..8577
                     /locus_tag="VIG33_07685"
     CDS             7801..8577
                     /locus_tag="VIG33_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270756.1"
                     /translation="MAKITKKNKSRSLKKRPELKARKTLGMPEANLLWVAISELIDHA
                     VSIESGQCEQKMIKGQREFILEVLNAKSKSDRHFRKYILNAQRVVMADRLTEVYRAFP
                     DNTENQLERKNQIETIAKQCELMFGKKLTKGEKLRLHAPLKDSNDPEDINFNFRGAKV
                     NAYDRLGDIFDKGSKSTLNTFKKTNLNGYESGPALDESDVMNHQKSRLKSIFIHLFKV
                     STERAETIASVLNAEEDFKRTDPFKEIRKIIQRLVFDSVQ"
BASE COUNT         2384 a         2115 c         1781 g         2424 t
ORIGIN      
        1 actccgcacc tgcaaggtga ataaaaagct cagctaatgc tctaagttct tttgcgcatt
       61 caaaacttat ttgtgctgag taggaaaccc tagcggcccc accgccggct aacacataac
      121 cgtcgcgtgc gttcgtcgca aacatgggat ccaccttgta tgcaatatag ggactgtcta
      181 aatctacttt catttcaatc tcctgttggt ctgaaaagtt cattttgtca atctttgagt
      241 aaacggaatc acattctgag gaacccagtt tttctcgatg taataaggat tcaacttaat
      301 gtcggcaatc catggttggc catattcaat ttgtttccac ggcactttaa tttcctgagc
      361 tctatctata agtcttttaa ttccaatctt attccacgct ggtaaagtga ccgtttctga
      421 aaggtgtctg cctttaaata tcctcgcgtg atattttttc tcattgggag aaaatgttac
      481 ttctatcctg acattccgat cgtatgttgt tcgaatcttc tcaattcgac ggcgccaaga
      541 atccatgtca tctcccctaa tgggatttct ctttattgct tttttctgat cagaggttaa
      601 ctcgccgagt tcacctatca cctgaagatg atctctctcg tgtttttttt gttcaatgat
      661 tctctgcttc agcaattcga ctgtcaccgc ctgcttttca gcttttttgt ataaatagtc
      721 caagcgctct tcctgccact caagctccat atttaataac tcagagtcgg aatgtaacgc
      781 cgagcaatct acacaataca aatgtccata cactcgccgg cctgatccaa tcaaccttgg
      841 gtcctggcga catctccgac agtaatgatc tgggttatat gtgttttcac tcacagttta
      901 actcctcaat ttgaaacgca ttgattaatt tagacttgaa ttaaattgcc gtttcaagcc
      961 attcgaatat ttccgcaggt ggtttaagtc cacggatttc atagagccaa gtaacaaact
     1021 ctcgttcact catttcaagg cgagtgggat gcatcctctt tgccactgaa tttagcgcag
     1081 ttactttcca ccacgtaaac gtttgcccat acctgacagc gaaaacaggc actcccgcgc
     1141 gttcacccaa ctgagacagc gcaagataac taggatgact tggaaactgc gcagtcgcga
     1201 attcactttt atattcaatc aaagcagaag gtctgccctt gtcgtattca attagaagaa
     1261 agtccaaatc aacagcagga agtgctaaac cccacttacg atgccgttct gaaagatgac
     1321 catcacgcca cccggtccgc tctggcttaa cttgcttgat ctgtcgttga ccaatcgttg
     1381 cctcagcaag gttgtaggga gagggttcgc ttttaacaag ctcgacaggt gaggagcgca
     1441 tttcgaattc agattgctga ttttccatgc ctttgcttct ccttaatttc accaataatt
     1501 ttgccaattg atttatttct acaatcaact ccaagatttt tacttacgaa ctttaattct
     1561 cgaaatttct cactgataaa atcaatattc caaaccttaa ctgcttcatc gggtgttagc
     1621 tgcaggagtt catgttcaaa gaactctaca ggatctctag caggtggcgg aatgtccttc
     1681 cctccgctta ttattctaag ggtactcttt actttttcat ctcgaccaaa taaacgcgcc
     1741 acaagtattc ctttcgtata tataccacaa atgatgcgtc atatacatac attagatacg
     1801 tacatttaat ccgtataata ttgataggaa tgtaaatttt ttatacactt tcagtatggc
     1861 tataaatgca aaaacaatat tgaagcgatt agaagaaaat caaaacgaca ggggtaaggt
     1921 taccctttac ctttcaaaag gcctttttaa ggagtttaaa tctaaatgcg gtgatcattc
     1981 ggcctcagtc gttcttgaag agctcatgaa agagtttatt aactcgacaa aattaaatcc
     2041 aagaaggagc aagtaaaatg ccttacgcag caccattagt agatgacgaa aatgatccaa
     2101 ataaaaagag cttcggaaac cccgttaaca tttccggcca gtcgaccact atttcaacag
     2161 gaatacccgg acaagacgca agctccgtgc caggggtaaa acaaaagact tctggcaatt
     2221 ataccaatat ccaatcgtat cttgatgcca ataaagagca aggcgacacg atgggggcta
     2281 aaatcgcagg tgacgttacc agtaaagctg acgacgccac ctctaagatc ggggcctatg
     2341 aagcgaaagc gccaagtgtt caagcttatg atccaaatga agccataagt cgcgctacaa
     2401 gtttgagtga ctccgagaag gctcagtaca aaaccaacaa ggccacaggc ggttattctg
     2461 ggccccaaac tgcggatggg attgagggct atcaggatgc caataaagca ggcactgagg
     2521 cggtagcagc aatcaaaaat actggcactg aaagaggtca acgcgagctt ttaaaacaaa
     2581 cctacaatcg acctcaatat acagccgggg aaaataacct tgatcagacg ttggtacaaa
     2641 actcggcagg ctcaaaatct cgtcttgaac agattggccc aaaatacagc aagctttcag
     2701 attacttaag cggagcaaat gcgaaggtcg gaaatgcggt caacgcagcg aactctcagg
     2761 cgttcgcgaa taaacaggct tttattccag cagagcaatc tgcaaggact gctttagtca
     2821 gtccaattca ggcaagagct aatcaagcaa atcaaaacaa tccggcatat attaacagag
     2881 tactggacga tgcaagcgat aatgttattt cagaagaaac gctgaaggct ctgggcctta
     2941 atgaagggca agatcttttt gatatgaaac tgcgagatta tgttaaccag gattggtcac
     3001 cggtcggcat cgataacgct gcgactaacg atgagcgaaa taagtacgcg gcacttaatg
     3061 ccctttttga tgatccgact atgaatcaac ttacagtgga tggaaaagca atagaaccgg
     3121 taaagcttaa taaagagaaa tttgataagg atctggcagc aaaaaaagct gaatataacg
     3181 acgcttacac caatcaaaga ggaacgatat tcaatgagaa atacatgccc tatggtaaag
     3241 gcgatgttaa ccaatggatt ccgggcgcgt taaccgaccg aagagatttg agtacagcca
     3301 cggcatctga gttagaaaac tattggtttc ccctcttaga ccaagctcac caacaggctg
     3361 cggccgcagg gcaggcgggt gcccccggtg gcaactcagt ttatagttgg gcaaaaaacg
     3421 ccatgcaaca gtcactcaat gactggaaga acgcctatca agtgaaccga aaaattaata
     3481 aaggataact atgcgatcgg gattttgtgg cggctcctct caaataaagg atcgctgcaa
     3541 agtcccgaca gatgtcgcaa ctcacgcctt ctcttcatgg tctttcttct tagccatctc
     3601 aataagaaat tcattaggga tatgagcagt catctccaga acgtcctcaa tcgtcaaaat
     3661 attcgtatca tctgaagaat cggatttacc tactccccca cttgccggct taggctgaat
     3721 atacgaaaga agcattcccc acgccctcac ttgctcattt ggatcaagac ttggaataat
     3781 tgctaagatt tcttctgtgg ggttgacgtt cttttcagca agtacctcct cgactcttaa
     3841 cagacttttc ttattcggcg ttcctggttt tcgcccagac ccttcaactt ttttcattcc
     3901 ttttgcaaac atgcgccccc tcgaattcta cttttattct aaaagagttc taattcatgc
     3961 ctttacttca cggacaatga cttcaatttt cccatccttc ggcgcggcct tttcccagcg
     4021 gtaatcaggc cttccaatga tccgcatatt gtcgtcttta atgatgaggc acttcttgag
     4081 ggcatcgatc acgggcttaa aggaatgaac caggccatca tagtcaggcg ctcgccttga
     4141 gtgtcggatc agcgtcagat gagccttttc caagggcaaa agtggcgggc taagccttcc
     4201 tatcaacatt cggcctaaaa ctctcttatg ccacctctgg gattccttat atctcgtatg
     4261 ccaacttgcc ccaggggcct cattgattcg ctgagggagc ccgataagga tgagctcaag
     4321 tttataaagg ccactctcct tgtcgatcat attcttctcg ttacatggct gccataacat
     4381 tttgaatctc cttcccctga ttggtcttac ttccaccgat gggtccgcaa taggtctcga
     4441 tcaagaactg ctgacggttc aattctttaa atagaaagtt ccactcatgt ttaaaacagg
     4501 cgaaggcctc ggccctttta gccacaggtt gggcatagac ataagtttca attaaactga
     4561 tccggctctt taaaaactga aactgactgt gactcgcgat ctcatcgtag gagcaaaact
     4621 ccttcgctcc ctcgttatcg atcagtccaa gtcgattgct ctctggataa atctcccaga
     4681 cgttcttatt gccggacgca ttcgtcactg ggtcaccacg cttaagctga tagacccccc
     4741 tcaggtaatg aatcagatgc gctttgcgtt ccttgatttc ctcgcggtcc ctacctgtca
     4801 caagctggct tggccagtac accaatgcgt cgcccttgta agaaacctga accccttttt
     4861 taatgagcga actgattagt tgagcagggc tcatacggtc cccatgttaa atatgccttg
     4921 attttcatca tctgagccgt tcaagggggc caaaacaggg aagggggcca agggtgccac
     4981 gtgggaaagt tggccctctt cattttttgg cccacttggt atgctccaaa accattggtc
     5041 accgaccttg ttgcattcaa ccttaatctc ctttttagct cgcttcaggg tccgctcaga
     5101 gatcccgacc tcatttgcct cacgctgaat cttctttgcc ggcacctttc cataccggag
     5161 actttcgatt aaaaactcaa cggcttcttc aagcgccgat ctttcttcgg atccactcaa
     5221 attaagcgcc ataagatcat cagccgtgag ggcggtcggg ccggtgggaa ttaacacgcc
     5281 tttttcgatt ttgaagccca ggctttgccc gtagggggca cagttagact ttgtgtgaat
     5341 catcgcaagc tctgctgtat tctttggatt tgaacccaat aacacttcgc tccgagccgc
     5401 tccagtgatc ccgattgatc ccatgcctcg atagtttgct ttatcggcgc ccttctttag
     5461 atgccggaca cagatcacag cacacgagaa ccgcttggcc atgctttgaa tggcagtcat
     5521 gacttcagcc acctcattga ccttattcat gtccttacct ccaaagtaat tctgaagagt
     5581 atcgaacacg acaagagcca ctggactaag cgaactcaac acttcctcga cagtcacaag
     5641 cccatccgag ttcagctcaa aaggctctgg ataaacgtaa atctgcgaga gatcagcacc
     5701 gtgtctttca cactttggcc gtaaggtatc tgaagggtct tcttcacttg agaaaagtac
     5761 aatcttaccc tttttcattg tcactcccag caagagagat cgaacggcct gcgctaatag
     5821 cagaagctag cattgcgacg aatgtggact ttcctacccc tggatccccc gacatgagtg
     5881 agaccttccc gattgggaaa tacggctcaa ctaagaattc aacttgagtt gattgaacgt
     5941 catttaggca aatcagcttg gttttcaacc gttcccgtga agccagcaca tcatgagact
     6001 caacatcgaa agcctccgcg attatagcca ggagttgctc cttagtgccc cctgcatcaa
     6061 gccaatcaac tacgtcctct tttcctttaa gaccggggag atccactatc cgcgttgatt
     6121 ttgccacctc tttgatgtcc cgaaaggcat gacttgcata cttccttccg gcctcatcgt
     6181 tgtcgggcag aattgataca actttccccg cgaaagcctt tgcatagtgc tttttccatg
     6241 ctcccgctcc accaggcgtg gcagtcgcat tgagtccgag ttctttcaat ctttgagcga
     6301 ccttctcgcc ttcgccaaga atgacattgg gcttatcctt ccactctcga taaaagagcg
     6361 gagccacttc accctttggt gcttttggaa tccaggactc attttcaaga ctccattgcg
     6421 aaaaggattt cttccctccc gggttgtctt tgcgaatcac cttctgaata tcgttaccgt
     6481 taaagtcttg ataaatcgcg atagtctcaa tggccatatc gcttttactc gagtggttga
     6541 aacagggctt aggctcaagc agacctctat ctttcagcgc ggtatagact gcctcttgtg
     6601 agcaattggc gtgacactta aaaaggattt tcccctctga ttcattaact gaaagtgatc
     6661 ccgtttgatc atcgtgaaca gggcaatggc aaaggtatcc ttgcccaact ctttttcctc
     6721 cgagaatttc aaatattctt tcgatgctca tctctcacct cgagtagatt tgctcccgaa
     6781 ttctggcttt gaggcagcaa taaacttctt aagaaagcca aggcacgcct gggaaattaa
     6841 tttctcggca tcccttagat gtttcaattc cttcgaacac tcaacgtgtt ttgtcgtatc
     6901 catgcatgcg gcgagaatgt ttatcttgat attaacccga tcgaggatat tcaagaacgt
     6961 gcgatttatc tgggaaatct caacaatttt tgctaatttc attgcagagt cagatgttag
     7021 ttttcgagag gaacagtttg ccgctggcct ctcttttgtt tttaacttgg ccatgtttcc
     7081 cccttcattt cttcgatttc cagataaagc tcatcaatcg cctttagaag gcctcgatgt
     7141 aagatcttaa atggatcagc cttgtcggat acgtctctat ttggcagcgc ttgggtaatc
     7201 gcttcgagag tgcgaaaaag ttttcgagag tcagtcctag cggataccag cgcattctgg
     7261 tactcgtgac tttcagagtg cgggagactc atgcgacccc cttattagaa agaccggctt
     7321 gccaagactc tgcgctttcc ttagagatta aggttatgcg accacattta aagggtttga
     7381 gcctgccaga cttaatctcc tgatagattt tcgttcgacc gatgccgtaa atcttagcaa
     7441 attctccaat cctgtagttt cgcttttgac tatcgatcac agagcaccgc cgcgtgaatc
     7501 agacttcctt gaggctaacc agtccaaaac atccccccat cgataccgta cgcagcgagc
     7561 cgagatcttg acgaaattgg ggccgcctcc tttgcttcga tagccatccc aaaacctaga
     7621 gcttgtcccg gttcgcttcg ccacctcttt cgttgatata tgctcctgca ggtcatttaa
     7681 attggtgttc atattcactc tccttcatga acgttccgct tgtttctagt tggttttaca
     7741 tcagctagct ggtaagtact gaaaccaact tttggactga ttttttggaa gcaaacataa
     7801 atggctaaga tcactaagaa aaataaatca aggtcactaa agaagcgacc cgaattgaaa
     7861 gctcgaaaaa cccttggaat gcctgaagct aatttgcttt gggtcgctat atcggaattg
     7921 atcgatcacg ctgttagtat cgaatccggc caatgcgagc aaaagatgat caaaggccaa
     7981 agagagttta ttttagaagt tcttaatgcc aaatcgaagt cagataggca ttttaggaaa
     8041 tatattttga atgcgcaaag ggtggtaatg gccgatcggt taacagaggt ttatcgagct
     8101 tttcctgata atactgaaaa ccagttagaa cgaaaaaacc aaatagaaac tatcgcgaag
     8161 caatgcgaat taatgtttgg caagaaactt actaaaggcg aaaagctgag acttcatgct
     8221 cccctcaaag attctaatga tccggaagat ataaacttca acttccgcgg agcaaaagtg
     8281 aatgcctacg atcgacttgg ggacattttt gacaaaggca gcaaatctac tttgaatacg
     8341 tttaagaaaa ctaatttgaa tggatatgag agcggtcccg ctctagatga gtctgatgtc
     8401 atgaatcacc aaaaaagtcg tttaaaaagc atttttatac acttatttaa ggtttcaact
     8461 gaacgtgctg aaaccatcgc ttcggtcctt aatgcagagg aggattttaa gcgtacagat
     8521 ccattcaagg aaattaggaa gataatccaa cgcctggtct ttgattcagt tcaatgagtt
     8581 tcccgttttg attaatggaa atagagcaat actccgccca agaactcatt aagagttgtc
     8641 tcttatagaa aagatccccc ctgcgataag ccgcctcaac cttatttttc acaacgtgcg
     8701 ctaa
//
LOCUS       DATBTE010000208        10717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1939900, whole genome shotgun sequence.
ACCESSION   DATBTE010000208 DATBTE010000000
VERSION     DATBTE010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 10717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10717
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1939900"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VIG33_07690"
     CDS             complement(<1..309)
                     /locus_tag="VIG33_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270757.1"
                     /translation="MKILKKVLIIAISLIFFAYVAYQAFFKSPWSFHLSAPKIFFIIA
                     LTFFYSIGIGFVGMSWVSALQVKVPDLSMPYGLRVYFLAGIGKYLPGNLGHHVGRVWM
                     L"
     gene            481..1338
                     /locus_tag="VIG33_07695"
     CDS             481..1338
                     /locus_tag="VIG33_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8270758.1"
                     /translation="MSITVIIPCYNESIEVLKDTVTKTANSLSKTHPGAFEILVINDG
                     SKKVNYDSIDFAKLNARMIKHDYNQGYGASLMTGVMAAAHSWIGIADSDGTYPIERFH
                     EFMSYAEKYDMVIGKRPWKDIPLIRRPAKMILHKFACFMAGAEVEDLNTGMRIFRKEI
                     VLRYKGLFPKRFSFTSTLTMIHATSGQRVKAIDIDYAKRVGSSSIRPIADTIAFFSLV
                     LRLSLYFNPLKIFIPLSFFFLLSAIARGVRDYNLNSAFGGLTLVLFFMGFQLFFFGLI
                     AEVINRRST"
     gene            1423..2754
                     /locus_tag="VIG33_07700"
     CDS             1423..2754
                     /locus_tag="VIG33_07700"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEY8270759.1"
                     /translation="MKVSVVGTGYVGLVTGTGLAEVGNTVLCVDINKEKIENLKNGII
                     PIHEPGLKELVVENQKCGRLSFGSNLSEAIINTDVIFIAVGTPPLPNGKANLEYVYSV
                     AREIGKNINSYKAIVIKSTVPVGTGDEVHEIIHKTILERSVDVSFDVISNPEFLKEGA
                     AIEDFMKPDRIIIGTDSKRAREMMEKVYKSFFRQSERIIFMDRPSSELTKYAANAMLA
                     TRISFMNELSQLCEKSGANIASVRNGIGTDHRIGLHFIYPGIGYGGSCFPKDVQALMN
                     TAKDKGMTLGVLEAVHQANENQKTWFYEKIQTAFSSDLAGKTFALWGLSFKPGTDDVR
                     ESPAIDLAKRLLKAGAKVQAYDPVATNNAKKEFSEQDHIQFMSDSYHALEGAEALILA
                     TEWREFRSPDFMKIKELLKRPLFFDGRNQYNASDLKNLGFTYYSVGKTHGL"
     gene            complement(2827..7275)
                     /locus_tag="VIG33_07705"
     CDS             complement(2827..7275)
                     /locus_tag="VIG33_07705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8270760.1"
                     /translation="MKLLVQRSGYIFDPINKIWINPAYEGIAYSDSEEVETRIAHIIQ
                     QALDLSVFSTELRSHCTDWPSLYHLNGTRANILRPFKSFLKGDVLEIGAGCGAITRYL
                     GECGANVLALEGSPRRAAIARSRTRDLENVTVLAEKFDQFKCDQQFDVITLIGVLEYA
                     NLFTSGENPTLAMLERARSLLKPEGQLIIAIENQLGLKYFAGAPEDHLGQPMVGIEGR
                     YRRDQPQTFGRKVLADMLGQADFAKSEFFAPFPDYKLPVSILTEEGFSNKNFDAAVFA
                     WQSARRDPQLPSYCNFSLELAWPEVFKNDFGLDVMNSFLIVATPKIKQSVDTGVLAYH
                     YSTDRIPAYCKETLFIRRDKDIRVEYRGLGASQENADKEDNSLIKFICPTSDDYVLGK
                     LLSLEFISIVTADGWSFEQIAKFIWRYFLIVETFAKSAGIQVSMASPYAELSGEFFDV
                     VPQNIIIQKDGRAALIDKEWQLGRSLEAAHLLFRALLLLINSITRFGQHVTRERMTRS
                     QFMDGVFKAAGLSLNEADCARYIALEAEIQQQVTGRGAEIFLTWDKDQFLPIFNLSQA
                     AVEGEKQIASLNQMVVERGEWAQSLNRELTERDQLTQKLHGELTERDKLTQKLHGELT
                     ERDQLTQKLQGELTERDELIQKLQGDFTACDGQVAKLKQELAEWERTGIKQAQGLVDR
                     DAQIVKLNQALVECEASIKYFSEETVRRGEWALRLEAELKEERARVLAIAQSNSWRVT
                     LPIREVRRWVSSPKGQAKRYITAALRWVKLKYHLSTTTVLPVPVMTQTVSEQFDHSMD
                     FAKTIEIPVSKCPQISVIIPIYGQIGYTLQCLASIAANLPQASFEVIVVDDCSPDNSV
                     EVLTQVKGIRLIRNEQNQGFIRSCNLAASKAQGEYLHFLNNDTEVTPGWLDKLLSTFQ
                     EFPGTGLAGSKLVYPDGRLQEAGGILWQDGSAWNFGRFQDPLMPTYNYAREVDYCSGA
                     SIMVPKSLFDELGGFDEHYLPAYCEDCDLALKIRDNGYRVMYQPLSAIIHYEGVTSGT
                     DLSQGAKAYQVENTQKLYNRWKSRLQTYQPNGVDVDSAKDRQFSRRVLVVDHCTPTPN
                     QDAGSVLIFNLLLLLREMGFQVTFVPEDNFLFMPEYTPELQRLGIEVLYAPYVTSLEQ
                     HLKEFGKRYDLVFLVRPMVVEKQVSYVRKHCPQAKVLFHTVDLHFLRMSREAALQADK
                     AKLKMAAEMKQRELSAIRASDAAIVVSTAELELLCSELPNANLHVFPLIMEVQGTTRV
                     CSERRDIVFVGGYQHAPNVDAVKYFVGAVMPLLRKRLPGVRFYVAGSNPPVEIRALAC
                     EDVIVLGFVEELNPLLDKMRVSVAPLRYGAGIKGKIGSALAVGLPVVATSLAAEGMAL
                     TNGENVLIADGAEPFADAIVKIYQDVALWNRLSHKGLEFAESAWGGEAAWNILAGILS
                     TLGFHSEKKSRPLLLYNTSAASKPSPIQNSQLNTSVPCDNCQNNIH"
     gene            complement(7406..8551)
                     /gene="rffA"
                     /locus_tag="VIG33_07710"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(7406..8551)
                     /gene="rffA"
                     /locus_tag="VIG33_07710"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HEY8270761.1"
                     /translation="MMKEKRIPFNWPHMTGKELYYIAESHFQGRLAGDGPFTKLCHGW
                     LETRTGCSKALLTHSCTAALEMAALLLDIQPGDEIIMPSYTFVSTANAFVLRGGIPVF
                     VDIREDTLNLDERLIEAAITPRTRGIVPVHYAGVGCEMDTILSIAQRHGLKVIEDAAQ
                     GVMASYKGRALGSMGDLGAYSFHETKNVISGEGGALLVNDSQLALRAEIIREKGTDRS
                     RFFRGEVDKYTWQEVGSSFLPGEIIAAFLWAQFEDADRITQERLSSWRYYHEILQPLE
                     AKGILRRPIVPEDCQHNAHMYYILLAPEIDRQKVLNEFKRHDIWSVFHYVPLHSSPAG
                     QRYGRVHGTLNVTVDHSERLVRLPIWVGLTQQQQERVLAILQGAVCF"
     gene            complement(8566..10329)
                     /locus_tag="VIG33_07715"
     CDS             complement(8566..10329)
                     /locus_tag="VIG33_07715"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEY8270762.1"
                     /translation="MKRAFPSYSEFEPYKFALLRILFGAVLVLRALYIQKLLIPEEWF
                     SPIGIYSLMGLTAAILLTCGLFTQWSLLFLIFFMWHTGDGLLGTSTLGNDVAAMLGLL
                     LFLTNAGAYLSMDSLLLNQFRWARKVLLYLEDVTSPRKIALAKFITLLAYACVCVYSL
                     SMHLNEPAWTSGNAGPLLFTNNFMSSWHQFAEALFVQSEFSVHLARISLWTMILWFIS
                     VIPFTLLGGIFRLYTITWGVLFFLLALIVLNLGSLAQIELVFWAAIFWSKRGLDVQKK
                     LVLFFDDKCNLCDKLVQVLSFLDLFGRMKFSPLSQSASSLKNFNIHLEEALIDLYGGV
                     EASGEVQSGYDLYVLLSRNLVLLWPLFPVFYLGKLLKIGPLLYRFVARRRREIFGVCS
                     LSRPKIFRPSLEVSSKRGFLHSAIFLHVFLLAGGYLVAIPAPYIGIEGSPNVVQKAAH
                     YYGITPINVFNSGDLRMAENWFTLNSIDNKELVPLFTEFGSRLDMHISDRVYFGNTLR
                     FRRGVIGDNQCAFEKMRPSIEYLSKVYLHLKSAPSGEYTFKYDQYYQALPADEELFRG
                     KYRVQPKEVKCNTLFKVSYNS"
     gene            complement(10536..>10717)
                     /locus_tag="VIG33_07720"
     CDS             complement(10536..>10717)
                     /locus_tag="VIG33_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270763.1"
                     /translation="SRFFQEKVSREKFEATAELKISPWLNSFFFQLLKVELSLIRKGF
                     NFPIGGSRLVVAKKI"
BASE COUNT         3165 a         2507 c         2226 g         2819 t
ORIGIN      
        1 taacatccac actcttccca catgatgacc caagttccca ggcaaatact ttcctatacc
       61 cgcaagaaaa taaactctga gtccataagg catcgaaaga tctggcacct taacttgaag
      121 agcagacacc cacgacatgc ccacaaaacc aatgccgatg gagtaaaaaa aagtaagggc
      181 tataataaaa aatattttag gcgctgaaag atggaacgac caaggagatt taaaaaaagc
      241 ttgatatgct acgtaggcaa aaaagataag actaatagca atgattaaaa ctttttttag
      301 aatcttcatc ttcaattgtt ttacctcact tattttcttt tttcactttt ttcactttaa
      361 gacaactttt atgcaaagcg ctatttgaca tttcagccgt aaagagaaaa acttcgttga
      421 atcaaaatta tttttaggtg acccttccga gagaacaaac acttcaacca tggatcctac
      481 atgagtatta ccgtaatcat tccttgttac aatgagtcta ttgaagtttt aaaggacact
      541 gtgacaaaaa ctgcgaactc ccttagtaaa actcacccgg gtgcgttcga aattcttgtg
      601 atcaatgacg gctccaaaaa agtaaactac gatagtattg attttgcgaa actgaatgct
      661 cgtatgatta aacatgatta caaccaaggc tatggggcca gcttaatgac gggagttatg
      721 gccgccgctc actcttggat tggcatagct gactcagacg gaacctaccc cattgagcgc
      781 tttcatgagt tcatgagcta tgctgaaaaa tatgacatgg ttattggaaa aagaccttgg
      841 aaagacattc ccctgattcg ccgaccggca aaaatgattt tgcataagtt tgcctgcttt
      901 atggccgggg ccgaggtcga agatctcaat acgggcatgc gaatttttcg aaaagaaatt
      961 gtcttaaggt ataagggact ttttcctaaa agattttcat ttacctccac tttaaccatg
     1021 attcatgcga ccagcggtca acgcgtaaag gccatagaca ttgattatgc aaaaagagtt
     1081 ggcagttcca gcattcgtcc cattgcggac actatagctt tcttctcttt ggtcttaaga
     1141 ctcagccttt attttaatcc cctcaaaatc ttcatcccat taagcttctt cttcttactt
     1201 tccgctatcg ctcgaggcgt gagagattat aatttaaata gcgccttcgg cggactcacc
     1261 ctcgttctgt tttttatggg gtttcaactg ttcttttttg gcttaatcgc agaggtcatc
     1321 aaccgccgga gcacgtaaag ataagcattc ttgattttta ccactcgggt gaatattgta
     1381 atgctttgct aatttaataa cctttttttc gggagtacag gcatgaaagt gagcgttgtc
     1441 ggaactggat atgtgggttt agtaaccgga accggcctgg ctgaggtggg caatacagtt
     1501 ttatgcgtag atattaacaa agaaaaaatt gaaaatctca aaaatggcat aatacctatc
     1561 catgaaccag gccttaaaga gcttgtagta gaaaatcaaa agtgtggccg actttcattt
     1621 ggttcaaact taagtgaagc cataataaat acagacgtta ttttcatcgc cgtaggaacg
     1681 ccgcccctcc ccaatggcaa ggcaaatcta gagtatgttt acagtgtggc aagagaaatt
     1741 gggaaaaata ttaactctta taaagccatt gtgattaaat ccacggtgcc tgtgggcacc
     1801 ggcgacgaag ttcacgaaat tatccataag acaattttgg aacgctccgt tgacgtttca
     1861 tttgatgtta tttccaatcc tgagtttctc aaagaaggcg ccgccatcga agacttcatg
     1921 aaacccgatc gtatcatcat tggaacagac tcaaaaagag ctcgggaaat gatggaaaag
     1981 gtttataaat cttttttccg ccaaagtgaa cgaatcatct tcatggaccg tccctcctct
     2041 gaactgacta aatatgcagc caatgcaatg cttgccaccc gtatttcctt tatgaacgag
     2101 ctttctcagc tttgtgaaaa atctggtgca aatatagctt cggtacgaaa tgggattggc
     2161 acggatcatc gcatcggtct tcattttatt tatcccggga ttggctatgg tggctcctgt
     2221 tttcctaagg atgttcaagc cctgatgaat accgcaaaag ataaaggtat gaccttgggt
     2281 gtgttggaag ccgttcacca agctaacgaa aatcaaaaaa cctggtttta tgagaaaatt
     2341 caaacagctt tttcttcgga tctagccgga aaaactttcg ctttatgggg actgtctttt
     2401 aaaccaggaa cagatgatgt ccgggaatca ccagcaattg acttggcaaa acgtctttta
     2461 aaagcaggcg ccaaagttca agcctatgat cccgttgcga ctaataatgc caaaaaagag
     2521 ttttctgaac aggatcatat tcaatttatg tcagattctt accatgctct tgaaggggcc
     2581 gaggcattaa ttttagccac cgaatggaga gagttccgct ctcctgactt tatgaagata
     2641 aaagaattgt taaaaagacc tctctttttt gatggaagaa atcaatataa cgccagtgat
     2701 ttaaaaaacc ttggctttac gtattactcc gtaggaaaaa cacacgggct ttaatgtctc
     2761 aacagaggtt ttagatcttc cggccctgac cggaagatct ttctacaaaa gctacatgcc
     2821 aactcattaa tgaatgttat tttggcaatt atcacaggga acagaagtat tcagctgact
     2881 gttttgaatt ggtgagggct tacttgcagc tgaagtatta tacagcaaca ggggtctaga
     2941 ttttttttca gaatgaaagc ctaatgtgct caatattcct gctaaaatat tccaagccgc
     3001 ttcccccccc cacgcacttt ctgcaaattc caagccttta tgactgagac gattccagag
     3061 ggccacgtct tgataaattt tcacgatggc atcggcaaag ggctccgcac catctgcaat
     3121 caaaacattt tctccattag tgagggccat cccttctgct gccaatgacg ttgccacaac
     3181 tggcaatcct acagccaacg ccgatccaat cttgcctttt atgccagcac cgtagcgaag
     3241 gggcgcaacc gagacacgca ttttgtcgag taaaggattt agctcctcaa caaagcctag
     3301 gacaatgaca tcttcacaag ccaaagctcg aatctcgaca gggggattgc ttcctgcaac
     3361 gtaaaaacgc acccctggta agcgtttgcg caggagtggc attactgctc ccacaaaata
     3421 cttcacagca tccacattag gagcatgctg atacccccca acaaagacaa tatccctacg
     3481 ctctgaacag actctggttg ttccctgcac ttccataatc aagggaaata catggaggtt
     3541 tgcattgggt aattcagaac agagaagttc aagttcggcg gtgctgacaa caatagcggc
     3601 gtcggaggca cgaatggctg acaactcccg ctgtttcatt tcagcagcca tttttagttt
     3661 ggctttatct gcttggagtg ccgcctctcg ggacatgcgc agaaaatgca aatctactgt
     3721 atgaaaaaga actttggctt gcggacaatg tttgcgaaca tagctcactt gtttttcaac
     3781 caccattggc cgaaccaaaa agacgagatc atatcgtttt ccaaactctt ttaagtgctg
     3841 ttcaaggcta gtgacgtagg gggcataaag cacctcgata ccaagacgct gaagctctgg
     3901 agtgtattca ggcataaaga gaaaattatc ttcaggaaca aatgtcacct gaaagcccat
     3961 ttcccgtaat aggagtaaca aattaaagat gaggactgaa cccgcatcct ggttgggggt
     4021 cggcgtacaa tgatctacaa caaggacacg gcgactgaat tggcggtctt tagcgctatc
     4081 gacatctacg ccattgggct gatatgtttg aagccgactt ttccaacgat tgtaaagctt
     4141 ctgggtattt tccacttggt aagctttagc gccttgtgaa aggtccgttc cagatgtcac
     4201 gccttcataa tggataatag cagagagtgg ctgatacatc acacgatagc cgttgtcacg
     4261 aatcttgaga gcaagatcac aatcttcgca atatgcagga aggtaatgtt cgtcaaagcc
     4321 gccgagctca tcaaacaatg atttcggcac catgatagag gctccagagc aataatcgac
     4381 ttctctggca tagttgtagg taggcattag cggatcttgg aatcgcccaa aattccatgc
     4441 actgccatct tgccagagga taccgccagc ttcttgcaat ctcccgtcag gataaacgag
     4501 tttcgaacct gccaatcccg ttcctggaaa ttcttgaaaa gtactcagca atttatccag
     4561 ccatccgggg gtcacttcgg tgtcattatt taagaagtgc aagtactcgc cttgagcctt
     4621 actagctgcc aagttgcacg agcgaataaa accttgattc tgttcgttgc gtatcagacg
     4681 tataccttta acttgagtca acacctctac tgaattatca ggtgaacaat catccacaac
     4741 gataacctcg aatgaagcct gaggcaggtt tgccgcaatt gaagccagac attggagcgt
     4801 gtaaccaatt tgtccgtaaa taggaatgat aactgagatc tgcggacatt tcgaaacagg
     4861 aatctcaatg gttttagcaa aatccatgga gtgatcaaat tgctcagaca ctgtttgtgt
     4921 cattactggc acaggcaata cggtggttgt tgataaatga tatttgagtt ttacccaacg
     4981 tagcgctgcc gttatatagc gctttgcttg tcccttgggt gatgaaaccc agcgccgcac
     5041 ttcacggatc ggcaaagtga ctcgccaaga attactttgt gcaatggcca acactcgggc
     5101 acgttcttct ttgagctctg cctctaaccg caaggcccac tcgcctcttc gtaccgtttc
     5161 ttcgctaaaa tattttatcg atgcttcaca ttccaccaac gcttgattga gtttaacaat
     5221 ctgtgcatcg cgatcgacca acccttgcgc ctgcttaatg cctgtacgtt cccactcggc
     5281 taactcttgt tttagtttag cgacctgtcc atcgcacgct gtgaaatcgc cttggagctt
     5341 ttgtattaac tcgtcacgtt ctgttaactc gccttgcaac ttctgggtta actggtcacg
     5401 ctctgtgagt tctccgtgca acttctgagt taacttatca cgctctgtga gttctccgtg
     5461 taacttctga gttaactggt cacgttctgt caattctcga ttgagagatt gagcccactc
     5521 tccccgttca actaccattt gattaagact ggctatctgc ttttcacctt cgactgcagc
     5581 ttggcttaaa ttgaatatcg gcagaaactg atccttatcc caagttaaaa aaatctctgc
     5641 cccacgaccc gttacctgtt gctgtatctc agcttcaagg gcgatatagc gagcgcaatc
     5701 tgcttcattt aaactcagac cggccgcctt aaatactcca tccataaact gagaacgcgt
     5761 catcctttcc cgagttacgt gctggccgaa gcgggttatt gaatttatca gcaacaacaa
     5821 agctcggaat aacagatggg ctgcttcaag ggatctgccg agttgccatt ctttatcaat
     5881 aagagcggcc ctcccatctt tctgaatgat gatgttttga ggtactacat caaaaaactc
     5941 acctgatagt tctgcatatg gcgaagccat gctcacttga atgcccgctg acttggcgaa
     6001 cgtttccact attaagaaat agcgccaaat aaatttagcg atctgctcaa atgaccagcc
     6061 atctgctgtt acgatactga taaattcaag ggaaagtaat ttgccgagaa cataatcgtc
     6121 cgaggtaggg caaataaact taatcaatga attgtcttct ttatctgcgt tttcctgaga
     6181 cgcaccaagg cccctgtatt ccacacgaat atccttatca cgacggatga acagggtttc
     6241 tttgcaatag gccggaatac gatccgtgct gtagtggtag gccaatactc ctgtatcaac
     6301 actctgtttg attttgggcg tggcgacaat caggaaggaa ttcattacat cgagaccaaa
     6361 atcattttta aaaacttcgg gccatgccaa ttctaatgaa aagttgcaat aggagggcaa
     6421 ctgcggatca cgccttgcac tttgccaagc aaaaaccgca gcatcgaagt ttttattcga
     6481 gaatccttct tcggtgagaa tggaaacagg aagtttgtaa tcaggaaatg gggcaaagaa
     6541 ttcagatttt gcaaagtctg cttgacccaa catatccgcc aatactttgc gaccaaaggt
     6601 ttgtggttgg tctctgcgat agcgcccttc aatcccaacc atgggctgcc caagatgatc
     6661 ctctggagca ccggcaaaat acttcaagcc caactggttt tcgatcgcaa taatgagttg
     6721 gccttcgggc ttaagcagcg aacgtgctcg ctccaacatc gcaagtgttg gattttcacc
     6781 tgatgtaaac agattggcat attcaagcac cccaatcagg gtaataacat caaactgttg
     6841 atcgcattta aactgatcga acttctcagc taaaacggtg acattttcaa gatcgcgagt
     6901 tcgagagcga gctatggcgg ctctgcgagg gctgccttcc agcgctaaaa cattagctcc
     6961 gcattcaccc aggtagcgcg taatggcgcc gcagccagca ccaatctcca ggacatctcc
     7021 tttgagaaaa gacttgaagg ggcgcaaaat attagcgcgc gtgccgttta aatgataaag
     7081 cgaaggccag tcggtacaat gcgaacgaag ttctgtcgag aaaacagaaa gatccaaggc
     7141 ctgttgaatg atatgggcaa ttcgcgtttc aacttcttca ctatcactgt aggcaatacc
     7201 ttcgtaggct ggatttatcc agatcttatt gataggatca aaaatataac ctgaccgttg
     7261 aacaagcagc ttcatgcatc aacctctgta gaatacggcc ctgtcgacca ttactttttg
     7321 ttatttgatg agttttttca atatcgggca aggttttttt gccattggag cgtatttcta
     7381 attcaggccc ttgcccaaat caacgctaaa aacaaaccgc tccctgaaga atcgctaaga
     7441 ccctctcttg ctgctgctgt gtcaggccca cccagatggg caatcggacc aagcgctcgg
     7501 agtgatcgac tgtgacattc agagtcccat gcactcttcc ataacgttgg cctgctgggg
     7561 acgaatggag aggcacataa tgaaaaacag accagatgtc atgtcgttta aattcgttta
     7621 ataccttctg ccgatcaatc tcaggggcca gcaagatata atacatatgc gcattgtgtt
     7681 ggcagtcttc aggcacgatg ggacgccgaa ggattccctt ggcttccagc ggctggagga
     7741 tttcgtgata ataccgccaa ctggataaac gctcttgagt gattcgatcc gcatcctcaa
     7801 attgcgccca aaggaaagcc gcgatgattt cccctggcaa gaaagaagac cccacctctt
     7861 gccaggtgta cttatcaact tcacctcgga aaaaacgact gcgatccgtg cctttttcac
     7921 gaatgatctc cgcgcgcaac gccaactgag aatcattgac cagcaaggct ccgccttctc
     7981 ctgaaattac attctttgtt tcatgaaagc tataggcgcc caggtcgccc atactcccta
     8041 aagcacgccc cttataacta gccatgaccc cctgggcggc gtcttctatg accttcaacc
     8101 catggcgctg agctattgac aggatcgtat ccatttcaca gcctacaccc gcatagtgaa
     8161 ctggaacaat ccctcgagtg cgtggtgtaa tggcagcttc gatcaaacgc tcatccaagt
     8221 tcagcgtatc ttcccgaata tcaacaaaga ccggtattcc acccctcaat acaaacgcat
     8281 tcgcagtgga cacaaaggta taggaaggca taatgatctc atcacctggc tgtatatcca
     8341 acaacaaggc cgccatttcc agcgcagcgg tacaggagtg cgtgagcaag gctttgctgc
     8401 aaccagtgcg cgtctctagc catccatgac aaagtttcgt aaatggcccg tcgcctgcga
     8461 gtcgtccttg aaaatgagac tccgcaatat aatatagctc tttgcccgtc atatggggcc
     8521 agttaaaagg gatgcgtttt tctttcatca tttcggccta gcccattatg agttataact
     8581 cactttaaat agagtgttac acttaacttc ttttggctgc actctatatt ttcccctgaa
     8641 aagttcttca tcagcaggca aagcctgata atattgatca tatttgaaag tatattcgcc
     8701 ggagggggcg ctcttgaggt gcagataaac tttgcttaaa tattcaatgg aaggtctcat
     8761 tttttcaaag gcacactgat tatcgccaat gacaccacgg cggaaacgaa gggtgtttcc
     8821 aaaataaact ctgtctgaga tgtgcatatc cagtcgcgaa ccaaactctg taaatagcgg
     8881 caccaattcc ttattatcaa tactatttag cgtgaaccag ttttcagcca ttcttaagtc
     8941 tccactatta aagacattga tgggcgtgat gccgtaataa tgggctgctt tctgaaccac
     9001 attaggagat ccttctatcc ctatataagg agcaggaatc gcaaccaagt aacctccagc
     9061 aagcaaaaat acatgcaaga aaatggccga atgtaaaaag ccccttttac tggaaacttc
     9121 aagcgaaggt ctgaaaattt tcggtctgga taaactacaa actccaaaga tttcccgcct
     9181 tctccgcgcg acgaagcgat acaacagggg cccaattttt aataattttc ccagataaaa
     9241 caccggaaag aggggccata acaaaaccaa atttcttgaa agaagcacat aaagatcgta
     9301 cccagattga acttcaccgg aagcctccac tccaccataa agatcgatca gcgcctcttc
     9361 taaatgaata ttgaaattct ttagcgaact cgcactttga gaaaggggac tgaacttcat
     9421 acgtccaaag aggtcgagaa aagaaaggac ttgcaccaat ttgtcacaca aattgcattt
     9481 atcatcaaaa aacaaaacta gcttcttttg gacatccagg cctcgctttg accaaaaaat
     9541 cgctgcccaa aataccaatt caatctgagc taaagaacct agattaagca ctatcagggc
     9601 gagaagaaag aacaaaaccc cccaggtaat agtgtaaagc ctaaaaattc cccccaggag
     9661 tgtgaaaggt atcacggaga taaaccacag tatcattgtc cacagggaaa tcctcgccaa
     9721 atgaacactg aattcgctct gaacaaagag agcctcggcg aattgatgcc aagacgacat
     9781 aaagttatta gtgaataata agggccctgc attccctgag gtccaggccg gttcgtttaa
     9841 atgcattgag agagaataga cacagacaca ggcgtaagcc agaagcgtga tgaatttcgc
     9901 caacgcgatt tttctgggcg aggtgacatc ttccaaataa agaagaactt ttcttgccca
     9961 acgaaattga ttcagaagca aagaatccat tgaaagataa gccccagcat tcgtcagaaa
    10021 aagcaaaagc cccagcatag cagctacgtc attgcccagg gtgctggttc ccaacaggcc
    10081 atctccagta tgccacataa agaagatcaa gaataacaag gaccactgag taaagaggcc
    10141 acaggtgagt aaaatggcgg cggtcagtcc cataagacta taaattccga tgggtgaaaa
    10201 ccactcctct ggaatcagga gtttttgaat gtatagggcc ctcaagacga ggaccgcgcc
    10261 aaagagaata cgcaagagcg caaatttata gggttcaaac tctgaataac tggggaaagc
    10321 tcttttcaaa agaaaatcgc caaatttaaa aaggactgaa tggatccttt tattccatct
    10381 aaccagcaaa taaacaactg acagactaac aacgaaaaag ggaagcgaat tattcataca
    10441 ttccttaatc atgaaccatg tgcaaaataa ggagacaaaa aaatttaact catttttaga
    10501 gtcaaattca agaaaaactc ctcttatagg ataaactata ttttcttggc tacgaccaag
    10561 cgggagcccc ctatgggaaa attaaaccct tttctaatca atgagagctc aaccttaagc
    10621 aattgaaaaa agaatgaatt gagccaaggc gagattttta gttctgcagt ggcctcaaat
    10681 ttctcacgag acaccttctc ttgaaaaaat ctagaaa
//
LOCUS       DATBTE010000209        12223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_369771, whole genome shotgun sequence.
ACCESSION   DATBTE010000209 DATBTE010000000
VERSION     DATBTE010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 12223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12223
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369771"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2136
                     /locus_tag="VIG33_07725"
     CDS             <1..2136
                     /locus_tag="VIG33_07725"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEY8270764.1"
                     /translation="VSSPDGGLENAFPSRPQRVATPVDQLPQLLKQHFGFDSFRASQE
                     VVCRTVTEGKDVLLVMPTGAGKSICYQIPGIARGGTTLVVSPLVALIEDQVTKLQALK
                     FAAERIHSGRGREESRQVCQRYLAGELDFLFIAPERLSVPGFPEFLRKRPPTLIAIDE
                     AHCISQWGHDFRPDYRILGERLKELRPVPVIALTATATPTVQEDICRQLGLAAEARII
                     QGFRRNNISIEVAELSPGDRPDAIVSILKKAGRLPAIVYAPTRKKTDELTEVLSENLR
                     VGAYHAGMLAQAREKVQAQFLANELDVMVATVAFGMGIDKPNIRTVIHAAMPGSIEGY
                     YQEIGRAGRDGLPSQAYLLHSFADQKTHEFFFELNYPDEAKLQAIFDALKPQKISKED
                     IRIKVMDLDPRLDQETFDRALEQLWVHRGVIIDPDENMSRGQALWLKSYQLQRELKQK
                     QLKQMLSFATSGKCRMVYLVSHFGDQNDSGKSCEMCDICNPQQTQSLMQKRNLSVTEK
                     KSVAEILRLLQHESGLAAGRLFQLLSDAKISISRGEFEKLVTLLERKGWLESQEASFE
                     KGGQSISYRKLSLSRGNGKSTAYNLQAIEELEIVAAPPSEKKRKKRESSKRSKTRSPR
                     SKSSPRSNNNSGTGSPSKLFEPLREWRLKQAKRKNIPAFRILTDKALIAICELRPRSI
                     MELLEISSLNRKSITQYGDDILRVVHLHT"
     gene            2282..3481
                     /locus_tag="VIG33_07730"
     CDS             2282..3481
                     /locus_tag="VIG33_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY8270765.1"
                     /translation="MSTLKINKYLISEVKTSLEEVFFDGKYADKVIEACFKRNGNWTT
                     QERKFFAETIYGVLRWERLLVFIAGDEGLYRIMGTQWLRMGHDLPPWEEFEGLSYDFV
                     QRREKLAASSFATKQSIPDWMDELGRKELGSDWEKIILALNKPADFFLRVNALKTKPE
                     ELIAALAESNIVAEKVSPELPYALRVQDRKEIFQTESFRKGFFEVQDAGSQMIVPLLD
                     VQPGQRVIDACAGAGGKSLHMASLMKNKGRILSMDIKDWKLHELKLRARRGGVDIIET
                     RLIDSTKVIKRNKEQADRVLLDVPCSGLGVLRRNPDAKWRLAIEDIEELQKEQYQILT
                     TYSQMTKKGGQMVYSTCSILPSENEKQVARFLKEKGSEWTLEKELHLLPNIQGFDGFY
                     AALLKRH"
     gene            complement(3543..3833)
                     /locus_tag="VIG33_07735"
     CDS             complement(3543..3833)
                     /locus_tag="VIG33_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY8270766.1"
                     /translation="MTKADLINLIAEKSGITRVKAETVVNTIFDSMVEALMRDDRIEI
                     RGFGSFVNRKYDAYKGRNPRTGEVINVEEKKLPFFKVGKELKEDINKGPTKK"
     gene            complement(3911..5161)
                     /locus_tag="VIG33_07740"
     CDS             complement(3911..5161)
                     /locus_tag="VIG33_07740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY8270767.1"
                     /translation="MSKKKALPESLNICLVSNRFQILSRATDSGFLWPIARGLAKQGH
                     KVTVISTKSRIGKAEIIRDDVKTYYLQENGSAFKNLNFEEAALQKFSELHKQEPFHIL
                     HSLDNSGRRIARNKKNFNIAVAYDVEATQMSQLFSILGMSQETLSGLLTTAVAVTYKF
                     LTTFLGTDRKLLKTADGIFVNNPQQRIVLERYYLYPDFHIYTVPYGMELGDLSPKEES
                     YEYRKKWNLSSNAHVVVTISDMTEIRELQNLLRSFEKVAIKKPNAYLIVVGNGPYFKQ
                     IEFEMLNLALGNRVILPGALSSRDLIEFILMADVYVDMSSRSTGFEASLIEAMAQKKV
                     IIGSEVSPIANVVEDGIDGFLLRPADTESLASLLIEIFSGSLPTEEIGNKARQKVNDL
                     FDTQKMIRSVLDAYRKILLNTGMY"
     gene            complement(5166..6299)
                     /locus_tag="VIG33_07745"
     CDS             complement(5166..6299)
                     /locus_tag="VIG33_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8270768.1"
                     /translation="MAKIILVTEKVNSTSWPLALSLKAQQHEVIFLTSYGEEASHIDG
                     IRFMAYFKKWSFLEALRILPMLFTYEPQVLHILLDQSPMKGAHVVLASYAKSHPLIVL
                     TTSLLQFEKRLRLGSPVRLLLQESDIVTCPSVDSLAKLRGLSVRSKKQGRGILPPALD
                     LYESTLLQEPHYEEPEGELAAEETWAQHLQEETFFVIPCFEKDFSPDSIFFTRITRLA
                     EAHFVVLLGSNQDWPLRSRKLFEQWMADQGLKNRWMFSGALPRSLEKELLKQTECLIL
                     AGIKLSPQAISQFYLKAIQCDTPLALDSKQACVHAHLWQKNVNCWILDYESLDKEFSS
                     FLTKKSFKPPESLQAKLSEDHHLTDTPLNELNRLYTRALAQLK"
     gene            complement(6287..6739)
                     /gene="mutT"
                     /locus_tag="VIG33_07750"
     CDS             complement(6287..6739)
                     /gene="mutT"
                     /locus_tag="VIG33_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HEY8270769.1"
                     /translation="MTQADEKTRKLKNRKGHWIPVVAGFLKKDGKILVGQRPENNSLP
                     GQWEFPGGKIEPGETPEQALVRELQEELGIDVEVGDLKLACTHSYGEVGILILFYEIL
                     FWKGQPKAQHHMMLEWIHPEELKHRNIPEANRKILDRIYKALGIEWRK"
     gene            complement(6745..7722)
                     /locus_tag="VIG33_07755"
     CDS             complement(6745..7722)
                     /locus_tag="VIG33_07755"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEY8270770.1"
                     /translation="MKKPWLLLSPQRAHDLGPFGLKLYSLFHSGPTPVWKSFTWNNLE
                     FKNPLGLAGGMDKNAESLKEWWALGAGFVEIGTVTPKPQSANPGKIMDRDLERKALWN
                     KMGFPSHGAEEVYYNLLAHDYKPTPVFINIGKNRETANEAAVDDYAYLIHRLKDPADA
                     FVINISSPNTKGLRDLQNKQALTKLLTPLIKLSHADTSKPILLKLSPDLSDENLEEAL
                     NVAVDLGIDGFVLTNTTLSRPGGIAFPAEGGLSGAPLKPLSCQILQKSLQILGKHRSG
                     KLIISAGGAMTPEDVFERLQMGADLVEIYSALVFEGPGFFRNVAGRYSR"
     gene            complement(7737..9014)
                     /locus_tag="VIG33_07760"
     CDS             complement(7737..9014)
                     /locus_tag="VIG33_07760"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEY8270771.1"
                     /translation="MEFHKSEAHLDGPLFKNALQTVEEAAKLINCDPNVLERLKKPRR
                     CVTVSIPVRMDDYSVKVFTGYRVQYNATLGPYKGGIRFHQNVDLPEVVGLAALMTFKN
                     SVLGLPLGGAKGGVCVDPNQISRTEKQNLTRRYASEISSFIGPTKDIPAPDVGTDPQT
                     MAWFMDTYSQEQGGYAQPGVVTGKPVEIGGSLGRNHATGLGVVYVAEKSFEKMGMKMA
                     GSTIAIQGFGNVGSFAALFSHQRGAKVVALSDVSGGIFNGDGLNIPDVMEYIKINKVL
                     KGYPKSTAINNEELLMLKVDALFPCALEGQIDVHNVDNVKAKIIVEGANGPISANATK
                     ILHKRGVFITPDVIANGGGVIVSYFEWVQDAMSLFWDEEEVNNRLKTIIHRAFDVGFK
                     FHQEKNVDMRSAAMAVSVQRLEKAMLLRGLYPR"
     gene            complement(9157..10005)
                     /locus_tag="VIG33_07765"
     CDS             complement(9157..10005)
                     /locus_tag="VIG33_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEY8270772.1"
                     /translation="MRKTMSPFQNLIYKPGYVSAEEKAEVLEYLSTLYPIWEMRYSKN
                     NPPPPDQSQRRLLRPVYWLGNWQFACLNYYHPPKGILHRCVSAEPYPPVLAELVRKIE
                     AMVKSEFSPKDVPRGWHLNTCLINFYGDQIDKEGKSLDCARVGEHKDFEPGPVASVSF
                     GDKALFQFVESHGTQSQSKVVLQQWIEDRSLQLFGGEKFKKQLFHRVQRVEKKCGEKF
                     NLNVTEFETRRINFTFRFVPDEHIQPFFKLPSTAQEDVLEYMQALAKNSLFFQKHLLL
                     QQRQKA"
     gene            10096..11109
                     /gene="rfaE1"
                     /locus_tag="VIG33_07770"
     CDS             10096..11109
                     /gene="rfaE1"
                     /locus_tag="VIG33_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEY8270773.1"
                     /translation="MIIAAEQRQDLIQTLNSFKNKKVLIIGDVGLDEYVMGDVRRISP
                     EAPVPIVEVSEEDKRLGLAANVAQNVASLGGIPLMVGVVGHDAGALTLQEICHKAKVS
                     WDHMIYSDRRPTTRKMRIMTGAHHIVRVDYEMRKYLSAETEDKVLQRIKELIQEADVV
                     ILQDYNKGVISQSLVKRTVEICQQHHKKLLVDPTRSNPGEFYRGVDMIKPNYEEAVAI
                     SGLKFDELRENPNKILEVGRALQKKTGAKEVVLTRGKEGMTIFSGDKIDEVPTFARKV
                     FDVTGAGDTVIAALSLGVAAQLPLVKSCTIANFAAGVVVGKIGCVPCETSEIQEYIRT
                     AQS"
     gene            11201..11521
                     /locus_tag="VIG33_07775"
     CDS             11201..11521
                     /locus_tag="VIG33_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270774.1"
                     /translation="MDLERIQYRGFKPRGEVKKEATKIAQKFLEEFPIEGTAKVVIAD
                     QGEHFHISVVGISENKLFSSEAVHRKKNINGWPRTWQLGALVELLGDFVQQVRGSFKS
                     QDKP"
     gene            11710..>12223
                     /locus_tag="VIG33_07780"
     CDS             11710..>12223
                     /locus_tag="VIG33_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270775.1"
                     /translation="MFNAYENDASLNDLQGNPQYVETTTPPGKVNFYSMTLEDLKAFL
                     KGLGKEQFRAQQIFKWVYEQRITDVEKMSNLSKAFRAEIPDVLSFDLPPVLKHLKSVD
                     GTQKFLFDMKNGNSVEAVLIPSEDRLTLCISSEVGCNMACKFCFTGKQKLKRRLTAEE
                     IVGQFMQVHDR"
BASE COUNT         3358 a         2691 c         2898 g         3276 t
ORIGIN      
        1 gtttcctcac ccgacggggg acttgaaaac gcattcccat cccgtcctca gcgcgtggca
       61 acccccgtag atcaactgcc gcaacttctc aaacaacatt tcggatttga ttcgttcaga
      121 gccagtcagg aagtggtttg ccggactgtc accgaaggaa aagacgttct cctggtgatg
      181 ccgaccggag ccggtaaatc tatttgttat caaattcctg gaattgcccg tggcgggacc
      241 accttggtcg taagcccttt ggttgcgtta attgaagatc aagtaacaaa gcttcaagct
      301 ttaaaatttg cggcagaacg aattcactca ggtcgcggac gagaggaatc cagacaagtc
      361 tgtcaaagat acttggctgg cgaattggat ttcttattta ttgcgccaga gcgtttatca
      421 gtaccgggat ttccggaatt tctgcgcaag cgacctccga ctcttattgc gattgatgaa
      481 gctcattgca tctctcaatg gggacatgat ttccggcctg actatcggat tttaggcgag
      541 cgtctcaaag aattgagacc ggtgccggtg attgctctga ccgcgacggc gactcccacg
      601 gtccaagaag atatttgcag acaattgggt ttagctgcgg aagcaagaat tatccaagga
      661 tttcgtcgca acaatatttc cattgaggtg gcggaactct cgccggggga caggccagat
      721 gccatcgtct ctattttaaa aaaagcgggc cgattgccag ctattgttta cgcgccaacg
      781 agaaaaaaaa ccgacgaatt aacggaagtc ctttcagaga acttaagggt gggtgcttac
      841 catgcgggga tgctcgcgca agcgcgtgag aaagttcaag ctcaattttt agccaatgaa
      901 ttggatgtga tggtggccac cgttgccttc gggatgggga tcgataaacc gaacataaga
      961 acggtcatcc atgcggcgat gcctggaagt atcgaaggct attaccaaga gattgggcgt
     1021 gccggtcgtg acggattgcc aagtcaggct tacttacttc attcttttgc cgatcaaaag
     1081 acccatgaat tctttttcga attgaattat ccggatgagg caaaactcca agcgatattc
     1141 gatgcgttga aaccacagaa aatttcgaaa gaggatattc gtataaaggt tatggattta
     1201 gatcctcgcc tggatcaaga aaccttcgat cgagccctgg agcagctttg ggtccatcgg
     1261 ggtgtaatta tagatccgga tgaaaacatg agccgaggac aggctctatg gcttaaatct
     1321 tatcagctac agcgcgagtt aaaacaaaaa cagcttaagc aaatgctctc ttttgcgacc
     1381 tctggaaaat gtcggatggt ttacctggtt tcacactttg gggatcaaaa tgactctgga
     1441 aaatcttgcg agatgtgcga tatctgcaac cctcagcaaa ctcaatcatt aatgcagaaa
     1501 cgcaaccttt ctgtgaccga gaaaaagtcc gtggctgaaa tcttgcggct tcttcaacat
     1561 gagagcgggc ttgctgcggg aagacttttc caactattat cggatgccaa aatttcaatt
     1621 tcccgtggag agtttgaaaa acttgtgacc ttattggagc gcaaaggatg gctagagtcc
     1681 caggaagcaa gtttcgaaaa gggtgggcaa agcatcagct atcgaaagct ttctctttca
     1741 cgagggaatg gaaaaagcac tgcttataat ctgcaagcca ttgaggagtt ggaaattgtt
     1801 gcggctccgc cttctgaaaa aaagcggaaa aagcgcgaat cctcaaaacg ctcaaaaacc
     1861 agatccccgc gttcaaaatc tagccctcgt tctaacaata attcagggac gggcagccca
     1921 agcaaactat ttgaacctct gcgtgaatgg cggctcaaac aggccaagcg caagaatata
     1981 ccggccttcc gcatactcac tgataaagcg ctcatagcca tttgtgaact gcgtccgcga
     2041 tctataatgg aattgttaga aatttcgagc ctgaaccgta aatccatcac tcaatacgga
     2101 gatgatatcc ttcgagttgt tcatcttcat acttgagtaa ttagagccct atttaccttt
     2161 cctaatgcag taaaatactc ataaggctgc taaagggtgg cttttttctt tgagtcgata
     2221 taaagatccg gctgcataaa acaagtcaaa aaatgaaccc ctatttagcc ctcatggcca
     2281 ggtgtctaca ttgaaaatta ataaatacct tataagcgaa gtgaagacct ctttagaaga
     2341 agttttcttt gatggaaaat atgcggataa agtgattgaa gcttgcttca aacgtaacgg
     2401 aaactggacg actcaagagc gtaagttctt tgcggagacc atttacgggg ttcttcgttg
     2461 ggaacgcctt ttggtcttca ttgcgggaga cgaaggactt taccgcatta tgggcactca
     2521 atggttgcgc atggggcatg atcttccgcc ttgggaagag ttcgaaggcc tcagttacga
     2581 ttttgtgcaa cgccgagaaa aactggcggc gagttcattt gcgaccaagc agtccattcc
     2641 ggattggatg gatgaattgg gccgtaagga gctcggcagt gattgggaaa aaattatcct
     2701 ggccttgaat aagccagcgg atttttttct gcgggtaaat gctctcaaga caaagccaga
     2761 agaactcatc gccgctttgg ccgagagcaa tatcgtcgcc gagaaagttt ctcctgagtt
     2821 gccctatgcc ttgcgggtcc aagatcgaaa agagattttc caaacagagt ctttccggaa
     2881 aggattcttt gaagttcaag acgccggttc tcagatgatt gttcctcttt tggatgtcca
     2941 gcctggtcag cgcgtgatcg atgcctgtgc cggggctgga ggcaagagtc tgcatatggc
     3001 ctctctgatg aaaaacaagg gacgcattct ttccatggat atcaaagatt ggaaattgca
     3061 tgaattgaaa cttcgagcgc gtcggggggg agtcgatatc atcgagactc gtctgattga
     3121 ttctacaaag gtgattaagc gtaataaaga gcaggccgac cgggttttgc tggatgtccc
     3181 ttgctcaggc cttggtgttt tacgtcgcaa tcccgatgca aaatggcgtc tggcaattga
     3241 agacattgaa gagttacaaa aagaacaata tcaaattctc actacctatt ctcagatgac
     3301 taagaaggga ggccagatgg tgtattcaac ttgcagtatc ttgccttctg aaaatgaaaa
     3361 acaagttgcc aggttcttga aagaaaaagg ttctgagtgg acgttggaaa aagagcttca
     3421 tcttcttcca aatattcaag gcttcgacgg tttctacgcg gcactcttga aacgtcacta
     3481 gagaaatcca aaaacaacat tcagaatgaa aaaagggctt cttgcgaacc cctttttttg
     3541 acttattttt tagtgggacc tttgttgata tcttctttga gttctttgcc cactttgaag
     3601 aagggcaatt ttttttcctc cacattaatg acttcacctg tacgtggatt gcgccctttg
     3661 taggcgtcat acttgcggtt cacgaaggaa ccaaagccgc gaatttcaat acggtcatct
     3721 cgcataagtg cttctaccat tgaatcaaag atagtattta cgacggtttc agcttttaca
     3781 cgggtaatgc ccgatttttc agcaattaga ttgattaaat ccgcttttgt catggaatca
     3841 attatacaga ctcgaagtgg tctgactagt gaaaaaccct catggctcaa cgcaggaatg
     3901 gcggatttag ttaatacata ccagtattta agaggatttt tcggtaagcg tccaggacag
     3961 accggatcat cttctgagta tcaaagaggt cattgacctt ttgacgggct ttattcccaa
     4021 tttcttccgt tggtaaactg cccgagaaaa tctcaatcag caaacttgca agagattcag
     4081 tgtcagctgg ccgaagcaag aagccatcaa taccatcctc gaccacgttg gcaatcggac
     4141 tcacttcaga cccaatgata acttttttct gagccatagc ttcaataaga ctggcttcaa
     4201 atcccgtgga ccgagaactc atatcgacat aaacatcggc catcaaaata aattcaataa
     4261 gatcgcgaga gcttaaagct ccaggcagga tcacccggtt gccaagtgcc agattcagca
     4321 tttcaaattc aatttgttta aaataagggc catttccgac cacgatgagg taagcattgg
     4381 gctttttaat cgccaccttc tcaaaagaac gtaacagatt ttgcagttct cggatctcgg
     4441 tcatatcgga aatcgtcacc accacatgag cgttagaaga gagattccac ttttttcggt
     4501 actcataaga ctcttctttg ggagaaaggt ctccgagttc cattccataa ggaaccgtat
     4561 agatatggaa gtctgggtat aaataatagc gctctaaaac aatcctttgt tgaggattat
     4621 tgacgaaaat cccgtcggcg gtttttagca gcttgcgatc cgtgcccaga aaagtcgtaa
     4681 gaaacttata agtcaccgcc accgctgtgg tcagaaggcc gctcagagtc tcctgactca
     4741 tccctagaat tgaaaaaagc tgcgacattt gggtggcttc cacatcgtag gccacggcga
     4801 tattgaaatt ctttttgtta cgagcaatgc gccgacccga attatccagg ctatggagaa
     4861 tatgaaaagg ctcttgctta tgaagttcac tgaatttttg gagggcggct tcctcaaaat
     4921 taaggttttt aaaagcactg ccattttctt gtaggtagta agtcttaacg tcgtcacgaa
     4981 tgatttcagc tttgccaatg cgactttttg tggaaatcac ggtcacttta tggccttgtt
     5041 ttgcgaggcc gcgggcaata ggccacaaaa aaccgctatc agttgcacgg ctgagaattt
     5101 gaaagcgatt gcttacgaga caaatattca aagattctgg caaagccttt ttcttggaca
     5161 tgggattact ttaactgcgc cagggcccga gtgtaaagac gattgagttc attcagcgga
     5221 gtatccgtga ggtgatggtc ttcactcagc ttggcttgca aactttccgg aggtttgaag
     5281 cttttttttg tcaagaaaga ggagaattct ttatccagag attcataatc aagaatccag
     5341 caattgacgt tcttctgcca aagatgagca tgaacgcagg cttgctttga atcgagagct
     5401 aaaggggtgt cacattggat ggcttttaaa taaaactgag aaatggcctg cggacttaat
     5461 ttaatccctg ccaaaatcaa acattctgtt tgtttcaaaa gttctttctc taaagagcgg
     5521 ggcaaggcgc cggaaaacat ccagcggttt tttaaccctt gatccgccat ccattgttcg
     5581 aaaagtttgc gactgcgcag aggccagtct tgattcgatc ccaggagcac cacaaaatgg
     5641 gcttcagcga gacgggtaat gcgagtgaaa aagattgaat ccggagagaa gtctttctca
     5701 aagcagggaa tgacgaagaa agtttcttcc tgaagatgct gggcccaggt ctcttcagcg
     5761 gcgagttccc cttcgggttc ttcgtaatga ggttcttgca gtaaagtgct ctcataaagg
     5821 tccagggcgg gtggaagtat gcctcgccct tgttttttag agcgcacact taagccccga
     5881 agtttcgcca aggagtccac cgaagggcag gtgacgatgt cactttcttg aagaagcaaa
     5941 cgaacggggc tacccagtcg caaacgtttt tcaaactgta gcaatgaagt ggtgagcacg
     6001 atgagcggat gcgattttgc gtagcttgct aaaacaacgt gagccccctt catgggagat
     6061 tgatcaagaa gaatatgcaa gacttgaggc tcgtaggtga agagcatggg taaaatgcga
     6121 agggcttcga gaaaagacca ctttttgaag taagccatga agcgaatgcc gtcgatatgg
     6181 ctggcttctt cgccatagct cgtaagaaag atcacctcat gctgttgggc tttcagtgat
     6241 aaagccaagg gccaagatgt ggagttcact ttttcagtta ctaaaattat tttcgccatt
     6301 ctattccaag tgctttataa attcgatcta agatctttcg gttcgcctca gggatatttc
     6361 tatgctttag ctcttctggg tgtatccact caagcatcat atggtgctgt gctttgggtt
     6421 gtcctttcca aaaaagaatt tcataaaata gaattaaaat gcctacctcg ccataagaat
     6481 gcgtacaggc aagctttaga tctcccactt ccacgtcaat gccaagctct tcttgcagtt
     6541 cacgcaccaa ggcttgttcc ggagtctccc ccggttcgat ttttccccca gggaactccc
     6601 attggccagg taaagaattg ttttccggcc tttgcccaac aagaatttta ccatcttttt
     6661 taagaaagcc agcaaccaca ggaatccaat ggcctttgcg attttttaat tttcgcgtct
     6721 tttcgtctgc ctgtgtcatc aatcctacct agagtatctg cctgcaacat tacggaaaaa
     6781 accaggaccc tcaaaaacca gggcagagta aatttcaaca agatctgctc ccatttgcaa
     6841 cctttcaaag acatcttcgg gagtcatagc tccaccggca ctgattatta acttccccga
     6901 acggtgcttc ccaagaattt gcagactttt ctgcagtatt tgacaagaaa ggggttttaa
     6961 aggagccccc gaaaggcccc cttccgctgg gaaagcaatt cccccaggac gggaaagagt
     7021 cgtattggtc aggacaaacc catcaatgcc taagtccacg gcgacgttga gggcttcttc
     7081 aagattctcg tcgctaagat cggggctgag cttcagcaaa atgggtttgc tggtatccgc
     7141 atgagagagt tttatgagag gagttaggag tttggtgaga gcttgtttgt tttgcaggtc
     7201 acgcagtcct ttggtgtttg gactgcttat attaatcacg aaggcatcgg ccggatcttt
     7261 taaccgatgg atcaaatacg cataatcatc cacggccgcc tcattggcag tttcacgatt
     7321 ttttccgata ttaatgaaga ccggagtggg tttgtaatca tgggcaagaa gattgtaata
     7381 cacttcctcc gctccatgac tcggaaagcc cattttattc caaagggctt ttcgttcaag
     7441 atcacggtcc atgattttac cgggatttgc actttgcggc tttggcgtga cagtgccgat
     7501 ttcgacgaag ccggctccca gggcccacca ttcttttaag ctttcagcat ttttatccat
     7561 gcccccggca agtccgagtg gatttttaaa ttccaaatta ttccaggtaa aacttttcca
     7621 tacgggagtt ggtccggagt ggaaaagact gtacaacttt aagccaaaag ggcccagatc
     7681 gtgggccctt tgtggtgata ataatagcca tggttttttc atagcggaca cctttattat
     7741 cttgggtaca atcctctgag caacattgct ttttcaaggc gttgtacaga aactgccata
     7801 gcggcggaac gcatatcgac attcttttct tggtggaact tgaagcccac atcaaaagcc
     7861 cgatgaataa ttgtttttaa gcggttgttt acttcttctt cgtcccagaa caaggacatg
     7921 gcatcttgaa cccattcaaa ataagaaacg atcacaccac ccccattggc aataacatct
     7981 ggagtaatga aaacgccgcg tttgtgcagg attttggtgg catttgcaga gatagggccg
     8041 ttggcgcctt caacaataat tttagctttc acattgtcca cgttgtgaac gtcgatttga
     8101 ccttcaaggg cacaagggaa aagcgcatcc actttcaaca tcaacaattc ttcattgttg
     8161 atcgccgttg atttcggata acctttcaaa actttattga ttttgatgta ttccataacg
     8221 tccggaatat tcaagccatc gccattaaag atcccgccac tgacatcgct gagggcgacg
     8281 actttagcac cgcgctgatg agagaaaagg gccgcaaaag aacccacgtt tccaaagcct
     8341 tgaatcgcaa tcgtggaacc ggccattttc atgcccattt tttcaaagga cttctcagca
     8401 acgtaaacca caccgagacc tgtcgcatga ttgcgaccta aggatcctcc gatctccacc
     8461 ggcttcccag tcaccacgcc aggctgtgca tagccacctt gttcttgcga ataagtgtcc
     8521 atgaaccatg ccatggtttg tggatctgtc cccacatcag gagcagggat atcttttgtg
     8581 gggccaatga agctagaaat ttcagaggca taacgccgag tcagattttg tttttccgta
     8641 cgggaaattt gattggggtc tacgcaaacg ccaccctttg ctccgccgag gggtaaacca
     8701 agaactgagt tcttaaatgt cattagggct gcaagtccta cgacttcagg aagatccaca
     8761 ttttgatgga aacgaattcc acctttgtag ggtcctaggg tcgcattgta ctgcacgcga
     8821 taacctgtaa aaacttttac agagtaatca tccatacgca ccggaatact tacggtcaca
     8881 caacgacggg gtttttttaa acgttctaaa acattgggat cacagttgat cagcttggcg
     8941 gcttcttcaa cagtttgaag agcattcttg aacaaaggac catctaaatg agcttctgat
     9001 ttatgaaatt ccattcggtc taactcctta aaatcactag ggtgatccta cctgaaggaa
     9061 aaacaccgag tctactgaca aaacagtatg actcagtggt tctaggagcg aaaaattaaa
     9121 gcccagtcaa aagtcgaggt gaatgaaccc agagcgttag gccttttgtc tttgctgaag
     9181 gagaagatgt ttttggaaga agagtgagtt ttttgcgagc gcttgcatgt actcgaggac
     9241 atcttcttgt gcggttgaag ggagtttaaa gaaaggttga atgtgttcgt cgggaacgaa
     9301 cctaaaagtg aagttgatcc gacgagtttc aaactctgtc acattcaaat taaatttctc
     9361 gccgcatttt ttctccacgc gctgaactcg atggaaaagt tgttttttga atttttctcc
     9421 gccgaaaagt tgcaaagagc gatcttcaat ccattgttgc aaaaccactt tgctttgcga
     9481 ttgagtgcca tgactttcca cgaattgaaa cagggctttg tcaccaaagg aaaccgaggc
     9541 caccggtcca ggttcaaaat ctttgtgctc gccaacgcgg gcgcagtcga ggcttttacc
     9601 ctctttgtcg atttgatcgc catagaagtt gatcaagcag gtattcaaat gccagccccg
     9661 agggacgtct ttagggctga attcactttt taccatagcc tcaattttcc gtaccaattc
     9721 ggccagcacg ggaggatagg gttcggcgct cacgcagcga tgcagaattc ccttgggcgg
     9781 atgataataa ttcaaacaag caaattgcca atttccgagc caataaactg gacgcaataa
     9841 gcggcgctgc gattggtctg gcggaggcgg attgtttttg gagtagcgca tctcccaaat
     9901 aggataaagc gtgctcaaat actccaaaac ttcggccttc tcctcagccg acacgtaacc
     9961 aggtttataa atcagatttt ggaagggact catagttttt cgcatggccc cttttaacac
    10021 attgccgcac ttaaaagtat ggcttacttt taagtaatcg gtttaaaact gaacttttta
    10081 caagggagtt ggcctgtgat tattgcggcg gaacagcgtc aggatcttat acaaactcta
    10141 aatagtttta aaaacaagaa ggtccttatt attggtgacg tgggactgga tgagtatgtg
    10201 atgggggacg tgcgccggat tagtcccgaa gcgccagtgc ctatcgtgga agtgagcgag
    10261 gaagataaac gcttggggct ggctgcgaat gtggcacaaa atgtcgcgag cctggggggg
    10321 attcctttga tggtgggcgt ggtagggcac gatgcggggg cgctcacttt gcaagagatc
    10381 tgccataaag cgaaagtttc ctgggatcat atgatttact cagatcgccg tcctacaacc
    10441 cgaaaaatgc ggatcatgac cggtgctcat catatcgttc gcgtggacta cgaaatgaga
    10501 aaatacctgt ctgcagaaac agaagataag gttcttcagc ggattaaaga acttatccag
    10561 gaagctgatg tcgtcatact tcaggattat aataagggcg tgatctctca aagtttggtc
    10621 aagcgaactg tggagatctg tcagcagcat cataaaaaac tcctggtaga ccccacgcgc
    10681 tccaacccgg gcgagtttta tcggggtgtg gacatgatca agccaaatta tgaagaggcc
    10741 gtggcaattt ccggtcttaa atttgacgag cttcgggaga atcccaataa aattctcgag
    10801 gtaggacgtg cgcttcagaa gaagacggga gctaaggaag tggtccttac ccgcggcaag
    10861 gaaggcatga caattttttc aggtgataaa attgatgaag tcccaacctt tgcaagaaaa
    10921 gtattcgacg tgaccggagc gggtgatacg gtcattgcgg ccttatcttt gggggtggca
    10981 gcgcagttgc cattggtaaa atcctgtacc atcgcgaact ttgctgcggg agtggtggta
    11041 ggtaagatcg gctgtgttcc ctgcgagact tcggaaattc aggaatatat ccggaccgct
    11101 caatcttaga ctcccgacat tggtcttaag acccggtaaa aaaagtgcaa aaaaattaac
    11161 catttttatc tgtaggagta tcctaaaacg gggggctttt atggatttgg aacgcattca
    11221 ataccgggga tttaaaccaa ggggagaggt taagaaagag gcgacaaaaa tcgcccagaa
    11281 gtttcttgag gaattcccga tcgaggggac cgcaaaggtt gtgattgcgg atcaaggcga
    11341 acattttcat atctctgtag tgggcattag tgaaaataaa cttttcagtt cagaggcagt
    11401 ccatcgaaaa aaaaatatca atggctggcc tcgcacctgg cagttggggg ctttggtgga
    11461 attgttagga gacttcgttc agcaggtgcg tggatccttt aaatcgcagg ataagcctta
    11521 gtcctgcaaa ataatgaaaa attttagtgc cactttccct gctaaggcat ttcttaatta
    11581 tttttactca tttctgactc aatctctggc tattctccag ggcctagtgt aaggtctggc
    11641 ttctttgaaa aaccgacttc aaaaaaacct tttgaaaatg ctgtaacaag caagaaggaa
    11701 aactccccta tgtttaatgc gtatgaaaat gatgcttctt taaacgatct ccagggcaat
    11761 cctcagtatg tcgaaaccac caccccccca ggtaaagtca atttctactc catgaccttg
    11821 gaagatctca aagccttttt gaaaggctta gggaaagagc aattccgggc tcagcaaatt
    11881 tttaagtggg tctacgagca acgcataaca gatgttgaaa aaatgagcaa tctttcgaag
    11941 gcctttcgtg cagaaatccc tgatgtcttg agtttcgatc tgccgccggt tttgaagcat
    12001 cttaaatccg tggatggaac tcagaaattt ctttttgata tgaagaacgg caacagcgtg
    12061 gaagccgttt tgattccttc cgaggatcgg cttaccttgt gcatttcctc agaagtgggc
    12121 tgtaacatgg cttgcaagtt ttgttttacc ggaaaacaaa agctcaagcg tcgtttaacg
    12181 gcggaagaaa ttgttggtca attcatgcag gtgcatgatc gat
//
LOCUS       DATBTE010000210         1842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1734866, whole genome shotgun sequence.
ACCESSION   DATBTE010000210 DATBTE010000000
VERSION     DATBTE010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734866"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VIG33_07785"
     CDS             <1..674
                     /locus_tag="VIG33_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8270776.1"
                     /translation="ILEQIWHVTIESFATTALAGFFVGAIMTVQFTLQMKEFGALGFL
                     GGLATSATFREVGPLLIAFMLSGKIGAFTSAELGTMRVTEQIDAIRCLGANPIQEIIV
                     PRFLGIVISSFFLLFLGLFMSILGGMFLGFFFAGVNPEEYIRHIPTIITLPSIFSGLL
                     KCFVFGLVLATVCTQKGYSTTGGAKGVGRSVVSTAVITMIAIVVTDWFTSFIGEIILH
                     MIRGF"
     gene            675..1367
                     /locus_tag="VIG33_07790"
     CDS             675..1367
                     /locus_tag="VIG33_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8270777.1"
                     /translation="MTAQAKRFRWRETFEQLYFIGNGSLAIIVFCVSFAAMVTILESS
                     FHMKIVIQNDSMVPGFAAMLILRELGPVVTALLLTSRVGAGIAAEVGTMKVTEQIDAL
                     KMLGIFPVNYLVLPRLVACIVGAAFLTTVANMTCIFSAMVVSSSYLDYTPAMFITGMK
                     RFVQFKDLLFAIIKATCFGAVIPLISCYFGFHCESGAEGVGRATTNSVVAGSMAVIVI
                     DFILSYAFSYLY"
     gene            1409..>1842
                     /locus_tag="VIG33_07795"
     CDS             1409..>1842
                     /locus_tag="VIG33_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEY8270778.1"
                     /translation="MKIETKVGLLALGVMILIVTFAYMMGLISPFSSTYELNVMYNYA
                     GGIEEGSPVRVMGIKVGKVKKINFEPGYKSPKGEEVKLRVEITVDKKAWASLGKDSKF
                     FINLAGVIGEKFLEISPGSTELEGLKSGDYVRGEDPPRVDQL"
BASE COUNT          459 a          337 c          452 g          594 t
ORIGIN      
        1 tgattctaga gcagatttgg catgtgacta ttgaaagttt tgcgacgaca gccttggccg
       61 gatttttcgt cggcgctatc atgacggtgc aatttacgct gcaaatgaaa gagtttggag
      121 ccttgggatt tttaggcggc ttagcaacaa gcgcaacctt tcgcgaagta ggtcctttac
      181 taattgcctt tatgctcagc ggaaaaattg gcgctttcac ttctgcggaa ttaggaacga
      241 tgcgggtcac ggaacaaata gatgccattc gctgtttagg cgccaatccc attcaagaaa
      301 ttatcgttcc tcggttttta ggcatcgtta tatcgagttt ttttctatta tttttagggc
      361 ttttcatgtc cattttgggc ggtatgttct taggtttttt ctttgccgga gttaaccctg
      421 aggaatacat tcgccatatt cctaccatta tcacccttcc ttcgattttc agtgggcttt
      481 taaagtgttt tgttttcggg ttagttctgg ccacagtctg tactcaaaaa ggttattcta
      541 cgactggtgg agcaaaagga gtgggccgct ctgtggtgtc tactgcggtg atcacaatga
      601 tcgcaattgt ggtaacggat tggttcacga gttttattgg tgaaattatt cttcatatga
      661 tcagagggtt ttagatgaca gcacaagcaa agcgttttcg ctggagagaa accttcgaac
      721 agttatactt tattggcaat ggcagtcttg ccattatcgt tttttgcgtg agctttgcgg
      781 caatggtgac gattcttgaa tcctcatttc atatgaaaat agttattcaa aatgactcaa
      841 tggtcccagg ctttgcggcc atgctcattt tacgagaact tggtccagtg gttacggctt
      901 tacttttgac ctcacgagtt ggggccggaa ttgctgctga agtgggaacc atgaaagtca
      961 cggagcaaat tgacgctctt aaaatgttgg gtatttttcc agtaaattat ttggttttac
     1021 cccgattggt ggcgtgcatt gtaggagctg cctttctaac gacggtcgca aatatgacct
     1081 gcattttcag tgcaatggtg gtgagcagtt cctatttgga ttacacacca gcgatgttca
     1141 ttaccggaat gaaacgcttt gtgcagttta aggatctgct ctttgcgatt attaaggcga
     1201 cttgtttcgg agcagtcatt cctttaataa gttgttattt cggatttcac tgtgagtctg
     1261 gtgcagaagg tgtcggcaga gcaactacca acagtgttgt agccggatca atggcggtga
     1321 ttgtgattga tttcatttta tcttatgctt tcagttatct atactaagaa agatttaagc
     1381 cggatgctta aatttcaatg gagtttttat gaagattgaa accaaggtag gtttactggc
     1441 tctaggggtg atgattttaa ttgtcacctt tgcgtatatg atgggtctta tttctccttt
     1501 ctcgagcaca tatgagctca acgtaatgta taattacgcg ggaggtattg aggaaggctc
     1561 ccccgttcgt gttatgggta taaaagtcgg taaagtgaaa aaaattaact ttgaaccggg
     1621 atataagagc cccaaaggcg aagaggtcaa actacgtgta gaaattacag ttgataaaaa
     1681 agcttgggcc tctctcggca aagattctaa gttttttatt aatttggcag gggttattgg
     1741 ggaaaaattt ctcgaaattt caccaggcag cacggaatta gaaggactca agtccggaga
     1801 ttatgtgcgt ggggaggatc cgccacgcgt agaccagcta at
//
LOCUS       DATBTE010000211         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1325216, whole genome shotgun sequence.
ACCESSION   DATBTE010000211 DATBTE010000000
VERSION     DATBTE010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325216"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..1573)
                     /locus_tag="VIG33_07800"
     CDS             complement(293..1573)
                     /locus_tag="VIG33_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8270779.1"
                     /translation="MGNNKMVFLEKFKKLLPFANGQVYLFSAVLWLFSCQIGFYFLQI
                     NLANKTLFSLQNQLRQELDFSNFQYLIRSITDFQNSGSIKCAVVKMKSPVTMDILDLR
                     YMSGLNECAEFEWFLGGAHIRNELHSLNGDVYSFDFVSNNGIIFYFTTWSFRIFGILL
                     IFLAYKNMLLHVQAESNRMEVEMAYAKELSQIASMVAHDIRSPLSVLNVLVSNVSGLN
                     SEQKEIISAVVQRINSTADELLKKGRINYLKIPSTSQERKNINEQPKGVDTESCKIIP
                     TIEGLIQEKKLEYQNNSLVQIQFRPTQVSKDLSVSLSSSLISRIVSNLINNSTEAIID
                     DGSIFIEVEKREQFILVMITDFGKGMSEAILDTLKSRPVSYGKNGSTSGNGIGIFSAN
                     EALKKIGGNLQIQSKLDVGTKVTLSLPLDNGSRI"
BASE COUNT          545 a          335 c          298 g          561 t
ORIGIN      
        1 gtatttaatt ctgaattgag gccgacttcc atctcttcat gttaattccg tcgatactgg
       61 tccctcaacc tccggttata tcactggacc atgggaccgg taaaccggag aaaatagccc
      121 ctttttaaac cggcgcgtgc actaaggaaa gagaaagttt cttgaaatgt ttataaattg
      181 ttgaaaatcg cattcgaaga tcaggtcata tccaagacag attaagaaat cacaagcttt
      241 atgccactgc tttctccatt tcaagggcta agtgaacggc attaatgctc acctaaattc
      301 tgctgccatt atctagaggc aaggaaagag ttactttagt cccaacatct aatttagact
      361 gaatctgaag atttccgcca atttttttta gtgcttcatt ggcgctgaat ataccaattc
      421 cattacctga agttgaaccg ttttttccat agcttactgg acgcgacttc aaggtatcta
      481 aaatggcctc tgacatgcct ttaccaaaat cagttatcat aacaagtatg aattgctctc
      541 gtttttctac ctcgatgaaa attgagccat catcaataat cgcttctgta gaattattga
      601 ttaaattgga aacaattctt gaaattaaac tcgatgacaa tgatacgctt aaatctttag
      661 aaacttgagt aggtcggaat tgaatttgca ctaaggagtt attctggtac tcaagtttct
      721 tttcttgaat taatccttca atagttggta tgattttaca gctttctgta tcgacgcctt
      781 taggctgctc attgatgttt tttctctctt gagatgttga tggtattttg agataattaa
      841 ttcttccttt ttttagtaat tcgtctgcag tagagttaat tctctgcaca acggcgctaa
      901 taatttcttt ttgctccgag tttaaaccgg atacattact tacaagaacg tttaaaactg
      961 acaggggcga acgaatatca tgggcgacca tactagctat ttgcgaaagt tcttttgcgt
     1021 aagccatttc gacttccata cgattagatt cagcttgaac atgtaaaagc atgtttttgt
     1081 aggcaagaaa tataagtaga ataccaaata ttctaaaact ccaagtagtg aagtaaaata
     1141 taatgccgtt gttgcttaca aaatcaaatg aataaacgtc accattcaaa ctatgaagtt
     1201 cattgcgaat atgcgctccg ccgagaaacc attcaaactc agcgcattca ttgagaccac
     1261 tcatatatct taaatctaaa atatccatgg taaccggtga cttcatcttt acaacagcgc
     1321 atttaattga tccagaattc tgaaaatcag ttatgcttct tataagatat tgaaagtttg
     1381 aaaagtccaa ttcttgccgc aactgatttt gaagtgaaaa taaggttttg tttgctaagt
     1441 taatttgcaa aaagtaaaat ccgatttgac aagaaaagag ccataaaaca gctgaaaaca
     1501 aatagacttg tccattagca aatggtagta actttttgaa tttttctaaa aaaaccatct
     1561 tgttgttacc cattagtata caaatcgcgt ttcatcgcca atatgccacc ctacgaagtc
     1621 tcgcataaga catcgccata aatacacatc atcgatgttt gtagagaatt cttttaaaag
     1681 cgcgatctta gccatgggcc aaataggatt tgatgcccgc tctatttgtg caattgctt
//
LOCUS       DATBTE010000212         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_369881, whole genome shotgun sequence.
ACCESSION   DATBTE010000212 DATBTE010000000
VERSION     DATBTE010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369881"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..243
                     /locus_tag="VIG33_07805"
     CDS             112..243
                     /locus_tag="VIG33_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270780.1"
                     /translation="MKLLIVGKDKVFNKDGQINVKKAKIFLKAVGMVWQISDKMGQK"
     gene            520..888
                     /locus_tag="VIG33_07810"
     CDS             520..888
                     /locus_tag="VIG33_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270781.1"
                     /translation="MLARQELKLFRSWAPPSGGARDHFNLKTKDETLHFISMGGVHGL
                     GPDKTVPWKNNPNPQNPVMVDSYNFFAGSDYGYLAFMFQPLTNKWLLKSFKKNDKPDP
                     RNTSIGDAFKKALQLLKEDK"
     gene            885..1460
                     /locus_tag="VIG33_07815"
     CDS             885..1460
                     /locus_tag="VIG33_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270782.1"
                     /translation="MSNDLMCPACGSHELKALSEQKSHQLTLGGEFHYMEQMYKCASC
                     GEEGDFANRNDDVFKIAEHEALKKLVPSLIDDLSKEKISMAQFERAFELPQRTLTRWK
                     GGDFSASSVALLRTIKTFPFIINVAEKKFNPGFAQRSLFLEAFKLFGNVVQSQQGSIQ
                     VTLSASSDGASVRTDAYIPRAGSVTPTLRAV"
BASE COUNT          504 a          268 c          349 g          439 t
ORIGIN      
        1 ttgcaaaact agggggctat ataggacgaa ataatgacgg tccaccagga atgatctctg
       61 tttggagagg atggatggtg ctgatgcaat cggaaaaaac atggaggtca attgaagctt
      121 ttaattgtgg gtaaagataa ggtattcaat aaggacggcc agatcaatgt taagaaggcg
      181 aaaatcttct taaaagccgt cggcatggta tggcaaatct ctgataaaat gggtcaaaaa
      241 tagaagcggc catcagataa ctaatatcga ccgcgatgta tttctttgaa acaggcgaat
      301 ctgtttaggt ccggttttga cccttttact tcaaatcaaa tataggtggc ttttgcgcgc
      361 tttgcctcaa aatgacatag aataagatct tgacgcaacg aatagttaca tataatatta
      421 aaagatgtta cggatcgtta catctaccct tatgtttatg gaagaacaac cgaagaggat
      481 ggagagtgag ttcaagttac aaatgggatg atttcatgaa tgcttgcaag gcaggaacta
      541 aagctgttca ggtcatgggc cccgccgagc gggggggcaa gggatcattt caacttaaaa
      601 acgaaagatg aaacactaca ttttatcagt atgggtgggg ttcatggttt aggtccagat
      661 aaaacagttc cctggaagaa caatcctaat ccacaaaatc ctgtcatggt tgattcatat
      721 aatttttttg ctggttctga ttacgggtat ttggctttca tgtttcagcc actaaccaat
      781 aaatggctat tgaaatcttt taaaaaaaat gacaagcccg acccgcgaaa taccagtatt
      841 ggagatgctt ttaaaaaagc actccaactt ttaaaggagg acaaatgagc aatgatctaa
      901 tgtgtccagc atgtggctct catgagctta aggctctttc tgaacaaaaa tcacaccaac
      961 ttacgttggg tggagaattt cactacatgg aacaaatgta caaatgtgct tcttgtggtg
     1021 aagaaggtga ttttgcgaat cgaaatgatg atgtttttaa gattgctgag catgaagcct
     1081 tgaaaaaatt ggttccgtct ttaattgatg atttatcgaa agaaaaaata tcaatggctc
     1141 aatttgaaag agcgtttgag ctgcctcaaa gaactcttac acgatggaaa ggcggagatt
     1201 tctctgcctc aagcgttgcg cttctcagaa caattaaaac atttcctttc attattaatg
     1261 ttgcggaaaa gaaatttaat cctggattcg cccaaaggtc cctttttcta gaagccttta
     1321 aattgtttgg taatgttgtt caatcccaac aaggaagtat ccaagtaaca cttagcgcga
     1381 gttctgacgg agcttctgtt aggacagacg catatattcc gcgagcaggg tcggtaacac
     1441 caactctgcg agcagtatga aatgggtaga aaatgaaact tgaaacggtt attttctgtg
     1501 aagatattcg acatgagatc aataataaaa aatccttgat tgggatgtat catgatcgac
//
LOCUS       DATBTE010000213         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_233189, whole genome shotgun sequence.
ACCESSION   DATBTE010000213 DATBTE010000000
VERSION     DATBTE010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233189"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..674)
                     /locus_tag="VIG33_07820"
     CDS             complement(213..674)
                     /locus_tag="VIG33_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEY8270783.1"
                     /translation="MNFLAIDFETANYQRHSACSVGLVKVEAGVGTEKIVYIIGPPPR
                     MNLSLPTFMGFKWSDVALTDDFGKLWPRIQSLFEGIDFLAAHNASFDKGVLHACCQLY
                     KIEIPEIPFQCSMQLARKRLGIYPTKLSDVCRHLKNELNHHEALSDAHACT"
     gene            866..1324
                     /locus_tag="VIG33_07825"
     CDS             866..1324
                     /locus_tag="VIG33_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270784.1"
                     /translation="MEKLFFCLSILTAISANATSEVQTQVLAALIMNSGTIAMVDPTT
                     NKPLGRDEQIPALFAKALLQNYTEMGDHGGTLSSITITCDESDKVLKIGTAYKCSISI
                     GQGDFTKTESTMTGPETEGQILFDIDVVKPFDPKAKIIIKNKTVSVEYGD"
     gene            1520..>2146
                     /locus_tag="VIG33_07830"
     CDS             1520..>2146
                     /locus_tag="VIG33_07830"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEY8270785.1"
                     /translation="MKIVSISRFFFAFTILTSLVSFGHNAVCEGYKRSASLAKLLATS
                     KKMVAETQNHGARTIPNDPRRPLDRKANPEYNAIGIVKTDIGRGSGWLANECLVFTNK
                     HVIGAHKKIIGKKVMYHVGQTNTPEKNFEYAVEGEVVASGNPKGEGADSPAQDWALIK
                     LKKSVGKKVGFIDFAQYSVEDAETCKTLEIAGYPGEKSVHDLWWQSNCS"
BASE COUNT          649 a          441 c          481 g          575 t
ORIGIN      
        1 tggcatcggc tcggctaaaa cgtcgaaggc cgaggccgcc cactatgcga tggtgaatgg
       61 tccactctta aaccggctat ggtggaccac atgggaccgg taaaccggag aaaatggccc
      121 ctttttaaac cggcgcttcc attccttaag taaacatcat taattctaga tatgactgaa
      181 ttccgcggac ttacccattg cgcaagaata atttacgtgc aggcatgggc atcagacagg
      241 gcttcatggt gattgagttc gtttttcagg tgacgacaca catcagaaag cttggttgga
      301 taaatgccga gccgctttcg cgcaagctgc atcgaacact gaaagggaat ttccggtatc
      361 tctattttat agagttgaca gcaagcgtgc aaaactcctt tatcgaaaga ggcattatgc
      421 gcggccagaa aatcgatgcc ttcaaaaagc gattgaattc ttggccagag tttaccgaag
      481 tcgtcggtta aggcaacatc gctccacttg aagcccatga aggtaggtaa agacaaattc
      541 attcgagggg gggggccaat gatgtaaact attttctcgg taccaactcc tgcctccact
      601 ttcacgaggc ctacagaaca agcgctatgt ctttgataat ttgcggtttc gaaatctatg
      661 gccaaaaaat tcatcgattc tcccgaatat tgctgccata gtaaatagct ttttacaagc
      721 aaaggtcaaa aggtgagtgg ttaatattca atcgtgaatt gtttatactg tttgattttt
      781 tcagcggcac ttttaaacgg cccatgatag cactaatcgt tatataaaca aaatacttat
      841 aactgagtca aagaaggaaa accacgtgga aaaattattt ttttgtctaa gcatcctgac
      901 cgcaatttcc gccaatgcca ccagcgaagt ccaaactcaa gttttagccg ccttaataat
      961 gaactccggg acaattgcaa tggtcgatcc cacaaccaac aagccgctgg gtcgtgacga
     1021 gcagatacca gcactttttg ccaaggcact cctgcagaat tatacagaaa tgggcgatca
     1081 cggaggaacc ctcagctcta tcacgattac ttgtgatgaa tcagataaag ttctcaaaat
     1141 aggaacggct tacaaatgtt ccatctctat cggccaagga gatttcacaa aaaccgaatc
     1201 cacaatgact ggccccgaga cagaagggca gattttgttc gatattgatg tcgtgaagcc
     1261 gttcgatccg aaagcaaaaa ttattatcaa aaataaaaca gtcagcgtgg agtatgggga
     1321 ttaacattcg tcacaattca agcctcaaaa cgagattttt tcaggatttc taatataaga
     1381 agttctcctg gaagttctgt gccgtttttc gtctcacttt tgaggattgg acagatgcgc
     1441 aacccacaaa gacgcattgt cgagagtttg ccttggcaag atttttgaac tactagttag
     1501 ttgttaagtg aggtttttaa tgaaaattgt gagcatctca agatttttct ttgcatttac
     1561 aatacttact tcgcttgtct catttggaca caacgctgtc tgtgaggggt ataaaagaag
     1621 cgcttcactt gcgaaactcc tcgcaacctc aaaaaaaatg gtagcggaga ctcagaatca
     1681 tggcgctcga acaatcccga atgatccccg taggccttta gatcgtaaag cgaatcccga
     1741 gtacaacgct attggcattg tgaagacaga tattggtcgt ggatctgggt ggctagcaaa
     1801 tgagtgtttg gtatttacta ataagcacgt cattggtgcc cataaaaaaa ttattggtaa
     1861 aaaggtaatg taccatgtgg gccagacaaa tacgccagag aagaattttg aatatgcagt
     1921 agagggtgaa gttgttgctt ctggtaaccc taagggcgag ggtgctgaca gccctgctca
     1981 agactgggct ttaattaaat taaaaaaatc tgttggcaaa aaagtaggat ttattgattt
     2041 cgctcaatat agcgttgagg atgctgaaac ttgcaaaact ttagaaattg caggataccc
     2101 aggtgaaaaa agtgttcatg atctttggtg gcaaagtaat tgttca
//
LOCUS       DATBTE010000214         1773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1395130, whole genome shotgun sequence.
ACCESSION   DATBTE010000214 DATBTE010000000
VERSION     DATBTE010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395130"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="VIG33_07835"
     CDS             <1..1056
                     /locus_tag="VIG33_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEY8270786.1"
                     /translation="LCALSLAEAKKLPLEKLSLPPGFSISVFAKAPGARSLAVTPSGV
                     VFVGSRGLGDEHNVYRIKNNKVEIIASGFKSPNGVAFKDGKLYVSDISQVTEFTDPEN
                     KTGKDLNPRILPQKFPSDVHHGWKFIRFGPDGKLYVPVGANCNICDPGTEYARIFRID
                     VNGTSKEELAQGIRNTVGFDFHPQTKELWFTDNGRDWLGDDSPPDEVNHLTKIGQHFG
                     FPYCHGKNTQDPEFTQKKCADLTPPVVELRAHVAALGMRFYSGSSFPADYKDQIILAE
                     HGSWNRAKPQGYRLTLVRLKNGEAAKAEAFIEGWLQGADAWGRPVDVEMYKDGSLLVS
                     DDKAGVVYRVSYSPPKK"
     gene            1082..>1773
                     /locus_tag="VIG33_07840"
     CDS             1082..>1773
                     /locus_tag="VIG33_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HEY8270787.1"
                     /translation="MNNSVNKIIVSKFGGTSMGNAACMLRSAEVSLKQKSSVVVVSAT
                     SGTTNDLIELGKTSQAKPWPEAEIILNKIRQRHIKIAEDLKLSSEFGLQLSSLFEEME
                     SLGRGIHLLKDCSVKALDALMSLGERMSSVLFTAATQQVLQKYNSSKRAELFDVRKVL
                     RTDENFSKARPLTSEIKTLCKTHLGHIHSLETIVVTQGFIGRTEDGMTTTLGRGGSDY
                     SAAILAEGIGAD"
BASE COUNT          497 a          371 c          436 g          469 t
ORIGIN      
        1 ttatgtgccc tcagtcttgc agaagcaaaa aagttgccct tggaaaagct cagcttgcca
       61 ccaggtttta gtatttccgt ttttgcgaaa gctcccggtg ctcggtcctt ggccgtgacc
      121 ccctcagggg tggtttttgt aggcagtcgc ggactgggtg acgagcataa tgtctatcgg
      181 attaaaaata ataaagtcga aatcatcgca agcggtttta aatcccccaa tggggtcgct
      241 tttaaagatg gaaaactcta cgtgtcagat attagtcaag taaccgaatt tacggatcct
      301 gaaaataaaa caggcaaaga tctgaacccc agaatccttc cgcaaaagtt tccatcggat
      361 gtgcatcatg gctggaaatt cattcgtttt ggtccggatg gaaaactata tgtgcccgtc
      421 ggagctaatt gcaatatctg cgatccaggt acggagtatg cccgaatttt tcgcattgat
      481 gtcaacggca cttcaaaaga agagctggct caaggaattc gcaataccgt gggctttgat
      541 tttcacccgc agacgaagga actctggttt acggacaacg gccgggattg gcttggagac
      601 gatagtcctc cggatgaggt caatcatttg actaagatcg gccaacattt tggattccct
      661 tattgccatg gcaaaaacac tcaagatcct gaattcaccc aaaagaaatg cgcggatctg
      721 actccgcccg tagtagaact tcgagctcat gtggcagcgc taggaatgcg cttttatagc
      781 ggaagcagtt ttcctgcaga ttataaagat cagatcattt tggcggaaca tggttcttgg
      841 aatagagcaa aacctcaagg ttatcgtttg accctggtac gactgaaaaa tggagaggcc
      901 gcaaaagcag aagcttttat tgaaggctgg cttcaaggcg ctgacgcttg gggacgtccg
      961 gttgacgttg aaatgtacaa agatggttct ctcctggtat cagatgataa ggcgggggtc
     1021 gtctatcgag tgagttactc tccgccgaaa aaataagacg gagacttgag gggataaatc
     1081 tgtgaataat tctgtgaata aaattattgt ttcaaagttt ggtggaactt ccatggggaa
     1141 tgctgcctgc atgcttcgca gtgcagaggt gtctcttaaa cagaaatcaa gtgtggttgt
     1201 ggtctccgcg acctctggta ccaccaatga tttaattgaa ttgggcaaga cttctcaagc
     1261 caaaccttgg ccggaagctg aaataatttt aaacaagatt cgtcaaagac atattaaaat
     1321 agccgaagat ttgaaactct cttcagagtt tggtcttcaa ctgtcttctc tgtttgagga
     1381 gatggaatcc ttgggccgtg gaattcatct tttaaaagac tgctctgtga aggccttaga
     1441 tgcccttatg agtttaggcg aacgcatgtc ttccgtttta tttacggcag ccacccaaca
     1501 agttttgcaa aaatacaatt ctagtaaacg agctgaactc tttgatgtgc ggaaggtcct
     1561 tcgaaccgat gaaaacttca gcaaggcaag acctctgaca tcagagataa aaaccctttg
     1621 taaaacacac ttgggacata ttcacagcct tgaaacgatt gtggtgactc aaggatttat
     1681 cggacgtacg gaagacggaa tgaccaccac tttgggcaga ggaggcagtg attattccgc
     1741 agccatctta gcggaaggta ttggcgcgga tat
//
LOCUS       DATBTE010000215        16383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2420683, whole genome shotgun sequence.
ACCESSION   DATBTE010000215 DATBTE010000000
VERSION     DATBTE010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 16383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16383
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2420683"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VIG33_07845"
     CDS             complement(<1..446)
                     /locus_tag="VIG33_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEY8270788.1"
                     /translation="MALRQTMNLSQSLVITPQLQQAIKLLQMSRLELESAVRSELEEN
                     PILEEAEVLKESDLQRTKDAANEIDGGASEIVATNTQDPQKQDEFEWESYFEQNQKPP
                     QSGMAGSEEIMNYENAISASQTLHDHLSWQVKMNGFSEYEEKIADL"
     gene            complement(458..1180)
                     /gene="lptB"
                     /locus_tag="VIG33_07850"
     CDS             complement(458..1180)
                     /gene="lptB"
                     /locus_tag="VIG33_07850"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEY8270789.1"
                     /translation="MSYLQIKEISKAYKKRQVVDGASFSVESGQVVGLLGPNGAGKTT
                     SFYMVVGLVKPDSGLIELDAQNISAQPMYRRARIGLSYLAQEPSIFRKLTVSDNIIVA
                     LEAHGYRGSQRAEKLEALLSDFRITHIRDSYGYALSGGERRRVEIARALAGTPKFLLL
                     DEPFAGIDPIAVADIQNIIRELKAKGIGVLITDHNVRETLGICDYAYILKDGKIQVSG
                     SADEIANSELARKFYLGENFRL"
     gene            complement(1181..2140)
                     /gene="lptC"
                     /locus_tag="VIG33_07855"
     CDS             complement(1181..2140)
                     /gene="lptC"
                     /locus_tag="VIG33_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEY8270790.1"
                     /translation="MKNLKNILFVILLVFLFIEVLIIFPNRLEHKEEEAPVASTPLHS
                     TQPEQKMKGVHLVETHQGHRDWELFSAAAEGYQGKSAWELKNVKVLFYNKEKVSFTVT
                     GDLGSIDGKSKDLRIKGNVVTQSENGYTFKTSAIFYNSQKRQILSPEKVTMQGPKDEM
                     GDGFVLEGANMLVLVDDSQMTINQNVRGSKKFKDGKDFFITSDKADFSGQSHQAKFSG
                     NVEMKYANLHLKGPAAVFQYKGNTALINYIQMQGGVRVNDIDKVATADTVNLDLFKNI
                     FTFRGNPKVIQNNDELSGDEIIFLDGGKKVKVEKVRARMEKKD"
     gene            complement(2210..2800)
                     /locus_tag="VIG33_07860"
     CDS             complement(2210..2800)
                     /locus_tag="VIG33_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY8270791.1"
                     /translation="MAIFRPGERHRYHNILKRNKSKRGVTALLSLTAMVDMFTVLVIF
                     LLQNYNATGEVLFIPKEVILPKASMVKELKPAFVVTVSNKEVLLDKMRVATFEEVKGN
                     QEWMIKTLYEPLKAALDKKKAEMENGLNNKVKAVVINARGQEEQDPNSWRKITIQSDK
                     DVDFLTVKKVMFTVTEAGAGEINFAVMKRPNEPTSN"
     gene            complement(2802..3278)
                     /locus_tag="VIG33_07865"
     CDS             complement(2802..3278)
                     /locus_tag="VIG33_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY8270792.1"
                     /translation="MAHVEEGGKKRGRGRNVELNLVPVIDLMSVLITFLLITAVWSQV
                     NMMQIGSSLYGKQLDDKKPTPPPKSDVVLKIDIKETGYVLTVGRQVISLPVKSGVFDD
                     AGLMAQLQRVKQLYPEKTDAVVTMADDLPYEQLIRGMDDCLQSGFPAISVATGAPI"
     gene            complement(3304..4014)
                     /locus_tag="VIG33_07870"
     CDS             complement(3304..4014)
                     /locus_tag="VIG33_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY8270793.1"
                     /translation="MNPTATPIDAAAAAAMQAVSATENMNFVQRAFTEGGFVMYVIAL
                     IAIIAIFLIVERVMKFKAIAVDKKDFTDQIFRMILSGDLRQAISFCDARPAPITNTVK
                     AGLVQAMNKRPDEEIQVAMDAAVLREMPKVEGWSSFLAVFGNIAVLAGLLGTIMGMIG
                     SFRAVSAADPATKAQKLSEGISHALNCTAFGLSVAIISIIAYGLFQHQIQKTENEVIE
                     TSMSLLNLVVANRDKIKD"
     gene            complement(4088..6199)
                     /locus_tag="VIG33_07875"
     CDS             complement(4088..6199)
                     /locus_tag="VIG33_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY8270794.1"
                     /translation="MKSPVILRVFKGNQLVEVKQFDLDQIVIGHNADVGLDLNAEEVS
                     AIHCLIEMRDSGYYICDLGSTTGTFKNGQAVLDEPLASGDEIGIGPFKINFFVGIPKP
                     KALPPAQSEAPADSVVKAPPPQEVESVSIPAIIPATIPVTPSIPVISPVFEAPKVEPK
                     PHIATAPVVGPAVTKVETSSVKKSAFSRKGKNKKTYAPPSEVKDLRDQLRLGRGNVVE
                     VIVSWKERVLNTYHFRTPGIIRLGSEEKFEIHMPEGVCPTGWPLLEVGPETQVAVTSE
                     MKVELITNEGKKTVDDLLRMGKAQRTGNSVTMKLGQDEIYFIALPSSNLQIYVRFVQQ
                     PPVLAFPPWFLTSSELTGMIMSLILVALLALYVYTTAPSMVEEEKKEEIVAQVIFDKP
                     KPTPPPVKVVEEKPVEVPTPAPPKPTPTPKKVQLADKQVETTNKAKKPDPAMTAKNSK
                     AGAASEVAPKPNSQNKPKIFTSTQPQGGAVKQGKTESANAQSKNKDVSKVGMFAALGG
                     GGMRKNLDKAYSGAGEVLGTAANATGTAGSNSDRAGDDIGGKFKDTGAGGKGTATVGI
                     AGVGTKGRGTGYGTGDGLGDKNSVAIEPGGAEEDFVGTIDREAVRRVIRAGLREIRGC
                     YERELNKLNKTQRLEGKVIIEWTIADHGRALNAKVKSSTLGNRAVENCVRDRLASWKF
                     PDPPAGTVAEVNYPFYFRAEN"
     gene            complement(6196..6513)
                     /locus_tag="VIG33_07880"
     CDS             complement(6196..6513)
                     /locus_tag="VIG33_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270795.1"
                     /translation="MKRLLLSVITCSLVFFVSFTAFAQEEAAPAPAPTSGAPKSRAKG
                     TRTQKLNFEDELVEGGTQKPELFYLFQKKNFNYKRLIKLRQNFIPEMRKTSEDIQRIR
                     GGT"
     gene            complement(6534..7445)
                     /locus_tag="VIG33_07885"
     CDS             complement(6534..7445)
                     /locus_tag="VIG33_07885"
                     /inference="COORDINATES: protein motif:HMM:NF016611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8270796.1"
                     /translation="MMKKIVLASILSGFLTACGSSTKHGSGGEDSEYSPIANSSRGNN
                     SFDMGGDGASAASGKASQSFAQSAKAAPPITAAQAAVPLGQGVLVDAIKSQNDEHIFK
                     VASQLLTQNANDVKAMNAMAMVYFKKGKIDASKYLLNKAISVNPNAGEAYSNMGLIYL
                     SNNERREAIKAFRKALDINGNDSVAAANAGSIYAAEKDYNKAAMVLSVAYNGGVRDAK
                     TLNNYAVSLTAVGKFDEASSIYQAAMKDQNNNRELLLNYAILLIDHMNKYKEGMDVLN
                     RLKFVGPPSESRNRIIALENKVKAGVK"
     gene            complement(7442..10666)
                     /locus_tag="VIG33_07890"
     CDS             complement(7442..10666)
                     /locus_tag="VIG33_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8270797.1"
                     /translation="MINKKNRLLNSSLILSFLTGVVTYGVDAVALNKKQSTTTTATGG
                     KKTVGQLLSQISQESRGGNIQLHKGNTALPEFGYNFKPSQNQYNMESVKPPRSSEILL
                     NRGSGTKAEYERVLDQQVQELYKLTQRFKNSPNRGELWLRLAELYVEKAGIVDSRRQD
                     AYDQALADFKAGKTKVKPVLNTAEAKDFNKRAIQLYEWFERDFPKDEKMDQALFFLGY
                     NYFEMGDTHKGAEYYIRLNKQFPQSPFVGESHFSLGEYYFENEKWADAYKEYTYVIKD
                     KKHRLNTFALYKGSWCLFRLGKSAQALSYLEYIIKTAKYESEATSQSRRVVNKNRLEQ
                     EALRDIVIFYAEARPASDAIAYFRGLLPEGDIVPYLEKLAYYYSDKGGKDDARDVFKM
                     LIGERPESPRAFDWQYQIVQNYFYAKNSPKFREELYIWIKNFAANTPWYEANKANKEL
                     METSNKLRETTLRNYVMQQHQTAQNSHAALPMKTAYEGYGFYLEEFGNTPIAADMHFY
                     FAELLYDMKRYDEAAVQYKWVVDNAPQNKFMDKAAANLILALEKGVPSDEELQKRVAN
                     SLEPLPLDPKVERFIKASQWYITKFPKSEKAPEIKFRIGRLYYMHNQFDEATKVFRDI
                     LTIYPKTKYAEYSANLLLDIFNLRKDYAGLEKVGAELLTNQSIANSKVGGEIKSVMEK
                     ASFKKGQDLEVSKKYSESAQAFETFAKTNPRSELAVTAIFNAGVNYERAGMITAAIMM
                     YTQVLNSKDPKMESNKPKVRRLLAKLYQDTGNFEEAARLYKIAAAENPKDPLAPNFIY
                     NTALLNEALGKNDAAIHSYEEFIHMAKKKSETSDALYSVATLHRKANHLTAALQKYKD
                     YLASGANNPEKVVESHYWISDLLKKKHQVSESETWAKKTIAVQRRLAGDKKTGAQYAA
                     KLKFAEAQKSFNEYKAARISANPARQKETLDRKIAALGKFNKEIADVVKYDSAEEIVS
                     SLSLLGDANLHMYEAIMAVPLPSGLNAEETKQYKAGVEQMSSPFVTKAKESFKLAVDR
                     GWELEVYNEGYNTAMNQYTKLSPGSYYNNGEQTSDVRIINWMAQ"
     gene            complement(10670..12229)
                     /locus_tag="VIG33_07895"
     CDS             complement(10670..12229)
                     /locus_tag="VIG33_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270798.1"
                     /translation="MRKFITTFLTISFLFPAAYAAQKRLKSQGVAASAVKNSPLRQQV
                     NQALALAKNSQYEQASNALFQLARRAELQAERPQLKYVLGLMLMEMKLNQVAAFQFVD
                     VIRMGNNKYVKNAIEKLSIVADNLGDDTLLNYAVSKLDLNDFPSTNRDMLYFRIGEIK
                     MKAREFPKASELFSKVSQGSRYYNQALFNRGLAELEGNQIDTALQTFQTLMTVRDGAP
                     VTDTNRVAAQVAIARALYQKQDWDASIEAYAQVPRDHFLWHDALFESSWAMLRAARFR
                     SVLSNFQSLHSSYYDDFYIPESLLLRSIVYLYICKYDEMEKVLSLFENTYGPIRAKIG
                     DFIRINTDAMEYYAEIEKGQLIKKSDKAVNLRLPYNVVKNISEEGNVKRSLYYIKRLN
                     DEKARIDANPGFKNTPVGQYTLKVVNNRQRNAKLSVGDMVKAHMLNMRVELRDLYEQA
                     GFIRYEMINGKKEGLKMKLAGKDLAAEQIDEKIDRTFYVQNGYEYYPFQGEYWLDEVG
                     NYHYLGKQSCE"
     gene            complement(12226..13083)
                     /locus_tag="VIG33_07900"
     CDS             complement(12226..13083)
                     /locus_tag="VIG33_07900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HEY8270799.1"
                     /translation="MQTHFKTYCNTQCRILLMASVGVLLMMPLAKAQQDSSSAIGDDL
                     NVIEMELDKTAPRPPPTAAPSMQNQKLEANEDKLEAQSNKLTDFSGLTKLQPFSEVSV
                     IQKRFLPKTGRMQFTGAFSTITNNPWFLTAGVSLKAAYYFTEAWGLEGTYTSMTSSQR
                     TSAQELHDNNGVSADSFGFPKNYIGVDAKYTPFYGKMSWLNRKIIPFEHYFAVGGGNT
                     SVSTGGSGFTLHFGTGQNFALSKRFSMNWDLSMMNYSAKSTDGTMQSFSDLLLTVGMS
                     FFYPEATYR"
     gene            complement(13095..13892)
                     /locus_tag="VIG33_07905"
     CDS             complement(13095..13892)
                     /locus_tag="VIG33_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HEY8270800.1"
                     /translation="MIKNALKGLSFFLSFFLNFFLVAFFLQSVAFATEVVELPTEELA
                     TESVYPVFDKPVSVKNRNIITAGRFELGGYYGNALTEPIFNVSKYGLAGYYHVNENHA
                     IGAFFESNAAGLSKYGQQLTDVPGSTAGSHIHLDFTRAPAPQSTFMVDYNFKAFYGKM
                     SLSKSLVFNLTTFGSAAAGFVKYQNKSYLGIALGLGQKYHLSKQFSLRWDLRMFANNA
                     PIPFYSSTGNPYTLDPTDGVKKAPPFDQFSERLTYTTNLNVGVSYMF"
     gene            complement(13893..14417)
                     /locus_tag="VIG33_07910"
     CDS             complement(13893..14417)
                     /locus_tag="VIG33_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEY8270801.1"
                     /translation="MGPESIKLNLSLRNVKMLVLDVDGVLTDCRLWMDSDGEWKRFYS
                     IRDGVGIKRLIESGYKLAVITGSKAEDIRQRVKNLGIQFFYEGAADKVPSFLELQKVS
                     GFHVGEMAYIGDDIFDIPLLQEVAFGVTVPEAVEEVLEVARYVTKRPGGNGAVREVCD
                     LIFKHGAFSSSGRS"
     gene            complement(14493..15476)
                     /locus_tag="VIG33_07915"
     CDS             complement(14493..15476)
                     /locus_tag="VIG33_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEY8270802.1"
                     /translation="MSTKIIIQGRQVLDIEANAILALKERLSDGFVQAVQIIKASKGK
                     LILTGIGKSGQIARKLASTFSSTGTPSVFLHPAESAHGDLGIVESEDVILALSYGGET
                     PELAPILAFALRKDIQVIAITGNINSTLAKAAKAVLNVSVSAEACPLNLAPTASSTAS
                     LAMGDAMAMAVLNEKGFSPEEFAEFHPGGSLGYRLLTRVKDVMHTGDALPLVGLDTPM
                     RELFSIMTHKNVRGTAGVLDANGDLLGVITDGDIRRRLDKSPDPLTGTAKDLMTTTPR
                     GVDANELAEKALFLMEQFRIQILFVFDKLSDHPKKPVGMLNFQDLLRTKIR"
     gene            complement(15473..>16383)
                     /locus_tag="VIG33_07920"
     CDS             complement(15473..>16383)
                     /locus_tag="VIG33_07920"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY8270803.1"
                     /translation="NVIAAQDSSFIYEVPLALHKEGLDELIVEKLQLQSHRMDLSGWK
                     KIVKVLQNPKQSVTIGVVGKYVDLKESYKSLNEAIVHAGIANNSKVNIVYVDSEKLTD
                     KNVAQYLGDVEGILVPGGFGARGVEGKISAIKFARERQIPFFGICFGMQLASIEFARN
                     VCGIQDATSHEFFVDSKTRNYVIDTMIEQRGSVNKGGTMRLGSFSCALTKGSHVYNVY
                     KSPLIHERHRHRYEYNNKYRALFEKNGMNATGVCKERNLVEIVELVDHPWFIGVQFHP
                     EFKSKPLAPHPLFVHFVKAALKRGSE"
BASE COUNT         4348 a         4005 c         3332 g         4698 t
ORIGIN      
        1 aaaagatcgg cgatcttttc ttcatactcg ctaaaaccat tcatttttac ttgccaggaa
       61 agatgatcat gcaaagtctg agacgcggaa atcgcgttct cataattcat gatttcttca
      121 ctgccggcca tgccggactg aggaggcttt tgattctgct cgaaatagct ttcccactca
      181 aattcatctt gtttttgggg gtcttgagta ttggtagcga cgatttcgga agctcccccg
      241 tcgatttcgt tagcggcatc tttggttctt tgcagatcag attctttaag gacttccgct
      301 tcctcaagaa tagggttctc ttcgagttca gaacgaactg cagactcaag ttccagacgt
      361 gacatctgca acaacttgat cgcctgttgc agctgcggcg taatcaccaa ggactggctt
      421 aagttcatcg tttgacgaag agccacggaa atatctccta aagtctaaag ttttcaccca
      481 gataaaactt acgggcaagt tctgagttag caatctcatc cgcactcccg cttacctgaa
      541 tcttaccgtc tttcaatata taagcatagt cgcaaatacc cagagtctca cgaacgttat
      601 gatccgtgat caaaactcct atacctttgg ccttgagttc gcgaataatg ttttgaatat
      661 ccgcaaccgc aatcgggtca attccggcaa agggttcgtc aagaagcaaa aactttgggg
      721 ttccagccaa agcccgcgca atttcaacgc ggcgacgctc cccgccagat aaagcatagc
      781 cgtaactgtc tcgaatatgg gtgatgcgga agtcagagag caaagcttcg agtttttcgg
      841 cgcgttgaga acctcgataa ccatgagcct caagcgccac aatgatatta tcactcaccg
      901 tgagttttct aaaaatgctt ggttcctgcg ccagataact gaggccgata cgagcccgcc
      961 gatacatggg ctgcgcagaa atattttgcg catccaattc gatcaggccg gaatctggct
     1021 ttactagacc aaccaccata taaaacgaag tggttttgcc tgcaccgtta ggacctaaaa
     1081 gccccacgac ttgcccggat tcgactgaaa aagaggcccc gtccacgact tggcgctttt
     1141 tataggcttt ggagatttct ttaatctgca gataactcac ttaatccttc ttttccattc
     1201 ttgctcggac tttttctacc ttaacctttt ttccgccatc aagaaagatg atttcatcgc
     1261 cagaaagttc atcgttattt tgaataactt ttggatttcc gcgaaatgta aaaatattct
     1321 taaaaaggtc caggtttaca gtatccgccg tagcgacctt atcgatatca ttcacgcgaa
     1381 cacccccctg catctgaata tagtttatga gagccgtatt tcccttatat tgaaaaacag
     1441 ccgcaggacc ttttaaatgc agattcgcgt acttcatttc cacgttgccc gagaattttg
     1501 cttggtggga ttgaccagaa aaatcggctt tatcactggt aataaagaag tcctttccat
     1561 ctttgaattt tttggagccc cggacattct gattgatcgt catttgactg tcgtcgacca
     1621 gaaccaacat attggcaccc tcaagaacaa aaccatcgcc catctcatcc ttagggcctt
     1681 gcatggtaac cttttcaggg cttagaatct gccgtttctg cgagttgtaa aaaatcgcgg
     1741 acgttttaaa agtgtatcca ttttcagatt gggttaccac attcccttta atcctaagat
     1801 cttttgattt cccatcgatg gaacctagat caccggtcac cgtaaagctc actttttctt
     1861 tattatagaa caaaaccttc acgttcttaa gctcccaggc agatttacct tgataacctt
     1921 cggcggcagc ggaaaaaagc tcccagtcgc gatgcccttg atgcgtttct acaaggtgaa
     1981 cgcccttcat tttttgctca ggttgtgtgg aatggagagg agtagaggca actggagcct
     2041 cttcttcttt atgctccagt ctgttgggaa aaataataag aacttcaata aaaagaaata
     2101 ctaggaggat cacaaataaa atatttttga ggttcttcat caatcacgta ccgcttacta
     2161 tcaatgagcc agcacgacct caaaaatcag tcacttttaa gtcatcaaac taattcgaag
     2221 tcggctcgtt ggggcgcttc ataaccgcaa aattaatttc tccagcgcca gcttcagtca
     2281 ctgtaaacat gaccttttta accgtcaaga aatccacgtc cttatcactt tggatcgtga
     2341 ttttcctcca agagttagga tcctgctctt cttgaccgcg agcattaata accacagctt
     2401 ttactttatt attaagccca ttttccattt ctgccttttt cttatccaaa gcagccttca
     2461 aaggttcata cagagttttg atcatccact cttgattgcc tttaacttcc tcaaaggtgg
     2521 caacccgcat cttatcaaga agaacttctt tattggaaac ggtgaccaca aaagcaggct
     2581 tcaattcctt gaccattgac gctttcggta aaataacttc tttaggaata aaaagtactt
     2641 ctccagtggc gttatagttt tgcagaagga aaatcaccag aaccgtgaac atatccacca
     2701 tcgcagtcaa tgacagcagc gctgtcacac cccgcttgct tttatttcgc ttaagaatat
     2761 tgtgatatcg gtgtctctca ccaggtctaa atatggccat attaaatcgg cgctcctgtc
     2821 gccactgaga tcgcagggaa tccagattgt aaacagtcat ccataccgcg gatcagttgt
     2881 tcgtatggca agtcgtcagc catcgtcact actgcatccg tcttttctgg ataaagctgc
     2941 ttcactcgtt gcaactgagc catcaagccc gcatcatcaa acacgccgct ttttacaggt
     3001 aaactaatca cctgtctgcc cacggtcagt acgtaaccgg tctctttgat atcgattttc
     3061 aaaaccacat ccgatttggg tggcggtgtc ggttttttat cgtcgagctg ctttccataa
     3121 agagagcttc caatttgcat catattgacc tgcgaccaaa ccgccgtgat caataggaac
     3181 gtgatcaaca cgctcatcaa gtcgatgacc ggaacgagat ttaactctac gttcctaccg
     3241 cgacctcttt ttttaccacc ctcttctaca tgtgccatgg ctttgaatcc ttaattaaaa
     3301 aaattaatct ttaattttat ccctgtttgc aaccactagg ttcaaaaggc tcatacttgt
     3361 ttcaatcact tcattttcag ttttctgaat ttgatgctgg aacaaaccat aagcaatgat
     3421 ggaaatgatc gcgacactca gaccaaacgc ggtacaattc aaggcatgcg aaataccttc
     3481 agaaagtttt tgcgctttcg tggccggatc cgccgctgat accgcgcgga aagagccgat
     3541 catacccatg atggttccaa gcaatcccgc caacaccgcg atatttccga aaaccgccaa
     3601 gaacgaagac caaccttcca ctttaggcat ttctcgaaga acagccgcat ccatggccac
     3661 ttgaatttct tcatcggggc gcttgttcat cgcctgcacg aggcctgctt tgactgtgtt
     3721 agtaatagga gctggacggg cgtcgcaaaa tgaaatcgcc tggcgtaaat cgccgctcaa
     3781 aatcatacgg aaaatttgat ccgtaaaatc ttttttatcg actgcgatag ctttgaattt
     3841 cataacgcgt tcgacgatca aaaaaatcgc gatgatcgca atcaaggcga tcacatacat
     3901 tacaaagccg ccctcagtga aggctctttg aacaaagttc atattttccg ttgcagagac
     3961 tgcttgcatt gctgcggcag ctgcggcatc tattggtgta gctgtagggt tcacgacttc
     4021 ctccttaagg atttaacttt tcttaaattt tttaacctta tactttcaac ctaatttaca
     4081 actcagctca gttctcagct ctaaagtaaa atgggtaatt aacctctgcc actgtcccgg
     4141 caggagggtc ggggaatttc caactcgcca gccgatctct tacgcagttt tcaaccgcac
     4201 gattgcccag ggtcgaactc ttcactttgg cattcagagc tcgaccatga tctgcaatgg
     4261 tccattcaat aatcacttta ccttcaagac gctgagtttt attgagttta tttagttctc
     4321 gttcgtaaca cccacggatt tcacgaagac ccgcacgaat tacgcgacga acagcttccc
     4381 gatcgatggt gccgacaaaa tcttcttcgg cgcctcctgg ttcgatggcc acggagtttt
     4441 tatctcccag accgtctcct gtaccgtaac ccgttccacg acctttcgtt ccgacaccgg
     4501 caatccccac ggtggcggtt cctttaccgc cggcgcccgt gtctttgaac ttgccgccga
     4561 tatcatcacc agcgcgatct gaattggaac ccgctgttcc cgttgcattc gcggctgttc
     4621 ccaaaacttc acccgctcct gaataagctt tatcgagatt ttttctcatc ccgccgcccc
     4681 cgagagccgc aaacattccg accttactga catctttgtt tttgctctga gcattcgcgg
     4741 attcagtttt accttgtttc accgcgcccc cttgaggctg cgtcgaagtg aaaatcttag
     4801 gcttattttg tgaattcggt ttcggagcca cttcagaagc cgccccggct ttggaattct
     4861 tagctgtcat cgccggatct ggctttttcg ctttattcgt agtctccact tgcttgtctg
     4921 ccaattgaac ttttttgggt gttggagttg gcttcggtgg agccggggtc gggacttcca
     4981 caggcttttc ttcaaccact ttcactggcg gaggagttgg tttcggctta tcgaagataa
     5041 cctgggccac aatctcttcc ttcttctcct cttcaaccat ggaaggcgct gttgtataaa
     5101 catacagcgc gagcaaggcg accaggatca aggacatgat cattccagtc agctcggagc
     5161 tagtcaggaa ccacggtggg aatgccaaga caggaggttg ctggacgaaa cgaacataaa
     5221 tctgcaaatt actagaaggt aaagcaatga aataaatctc gtcttgaccc aacttcatgg
     5281 tcactgagtt tccagtgcgc tgagcttttc ccatgcgcaa gagatcgtcc accgtctttt
     5341 ttccttcatt agtaataagc tcgactttca tctcagaggt caccgctacc tgcgtctctg
     5401 gacccacttc taatagtggc catcccgtgg ggcagacacc ctcaggcata tggatttcaa
     5461 acttttcctc agagccaaga cgaataatac ccggggttcg gaaatgatag gtatttaaaa
     5521 cacgttcttt ccaactgacg atgacctcta cgacatttcc cctgccaagg cgaagttggt
     5581 cacggagatc ttttacctca ctcggtggag cataggtctt tttatttttg ccttttcggc
     5641 tgaaagcgga ctttttcacg gaagaggtct cgacctttgt taccgcgggc cccaccactg
     5701 gagccgtagc aatgtgaggc ttcggttcaa ctttaggagc ttcaaataca ggagaaatca
     5761 ccggaatact tggagtgacg ggtatggtcg ccggaataat tgccggtatt gataccgact
     5821 ccacttcctg cggaggcgga gccttcacaa cggagtccgc gggggcttcg ctctgagcag
     5881 gaggcaatgc tttcggtttt ggaattccca caaagaaatt aattttaaaa ggaccgatac
     5941 cgatttcatc acccgaggcc agaggttcat ccaaaacagc ttggccgttt ttaaatgtcc
     6001 ccgttgtcga gcccaggtca cagatgtagt aaccagaatc tctcatctca atcagacagt
     6061 gaattgcgga aacttcctct gcattcaaat ccagcccgac atcggcatta tgaccgataa
     6121 caatttgatc aagatcgaat tgtttcacct caaccagctg attcccttta aatactctaa
     6181 gtataacagg agatttcaag tgccaccccg aatgcgctga atgtcttcag atgtttttct
     6241 catttcagga atgaagttct gcctcaactt tatcaaacgc ttgtagttga aatttttctt
     6301 ctgaaagaga taaaacaatt ctggtttttg tgttccacct tcaaccagct cgtcctcaaa
     6361 gttcaacttc tgagtgcgag ttcctttggc gcgagatttt ggcgctcctg aggtaggcgc
     6421 gggtgcaggt gccgcttctt cttgagcaaa agcagtgaag ctcacaaaga aaaccaagct
     6481 gcatgtgatc accgacagta aaagacgttt catgatcttc attccccctg tttctatttt
     6541 acaccagctt ttactttgtt ttccaaagca attatgcggt tacgggactc tgaaggagga
     6601 cctacaaact ttagtctgtt caggacgtcc atgccctctt tgtacttatt catgtgatca
     6661 attaagagaa tagcatagtt taaaagcaat tctcgattgt tattttgatc tttcatcgcg
     6721 gcctgataaa ttgaactggc ctcgtcgaat tttccgacag ctgtcaagga gaccgcatag
     6781 ttgtttaagg tttttgcatc ccttacaccg ccattgtaag ccacagaaag aaccatcgcc
     6841 gccttgttat aatctttctc ggcagcataa atggatcctg catttgccgc cgccaccgaa
     6901 tcattgccat tgatatcaag ggctttacga aaagctttga tggcctcgcg acgttcatta
     6961 ttagagaggt aaataagtcc catattgcta taggcctcgc ccgcgttcgg atttaccgag
     7021 atggctttat tgagaagata tttagaggca tcgattttac cttttttaaa gtagaccatc
     7081 gccatggcat tcatggcttt cacatcattc gcattttgag taagcagctg actcgccact
     7141 ttaaaaatgt gctcatcatt ttgacttttg atggcatcaa ccaaaacgcc ctggccgagt
     7201 ggaaccgctg cctgtgccgc agttattggc ggcgccgctt tcgccgactg agcgaaggat
     7261 tgagaggcct tccccgaagc agcggaagct ccatcaccgc ccatatcaaa actgttattt
     7321 ccccgcgaag aattcgcgat agggctatat tcactgtcct cacctccact cccgtgcttc
     7381 gtcgaggaac cacaggccgt gagaaaccca ctaagtattg aggctaaaac aatctttttc
     7441 atcattgcgc catccaattg ataattcgaa catctgaggt ctgctctcca ttgttgtaat
     7501 aagagcccgg actcagcttg gtatattgat tcatcgctgt attatagcct tcgttataaa
     7561 cttcaagttc ccaaccacga tccacggcta acttgaaact ctcttttgcc ttggtcacaa
     7621 atggcgagga catttgctca accccggctt tatattgctt ggtctcttcg gcattcaaac
     7681 cacttggtaa aggaaccgcc ataatggctt cgtacatatg gagattagcg tcgcccaaaa
     7741 gcgataagct gcttacgatc tcttccgcac tatcatactt aaccacgtct gcgatttctt
     7801 tattgaactt gcccagtgca gcgatttttc tatccaaagt ttccttttgt cgagctgggt
     7861 ttgcggagat acgagctgct ttgtactcat taaagctttt ttgggcctct gcaaacttta
     7921 atttggcggc atactgtgcg ccggtttttt tatcccccgc cagtcttctt tgaacggcga
     7981 tcgttttctt agcccaagtc tcagattcag aaacctgatg ctttttcttc agcaaatctg
     8041 aaatccaata atgggactct accactttct ctggattgtt agccccggaa gctaaataat
     8101 ctttgtattt ctggagggcc gcagtgagat gattcgcctt acggtgaaga gtcgccactg
     8161 agtagagcgc atcactggtc tcgctctttt tcttggccat gtgaataaac tcttcataag
     8221 aatgaatcgc ggcatcgttt ttacctaaag cttcattgag caaagccgta ttataaataa
     8281 agttcggggc taagggatct ttcggatttt ccgctgcggc tatcttatac aatctggcag
     8341 cttcctcgaa atttcccgta tcttgataca acttcgccaa aagccgacgg actttcggct
     8401 tatttgattc catcttagga tctttcgagt ttaaaacctg agtgtacatc ataatcgcgg
     8461 ccgtaatcat cccggctctt tcatagttca caccggcatt gaaaatcgcc gtgaccgcaa
     8521 gttccgagcg aggattggtt tttgcgaagg tctcaaaggc ttgggcggat tcgctatatt
     8581 ttttgctgac ttcaagatct tgacctttct tgaaacttgc tttttccatc acgcttttga
     8641 tttcgccgcc cactttggag ttggcgatgg attgattcgt cagcaactcc gcaccaactt
     8701 tttccaatcc cgcgtagtct ttacgcaaat tgaagatatc cagcaataag ttagctgagt
     8761 attcggcgta ttttgttttt ggataaatcg tcagaatatc gcggaaaacc tttgtcgctt
     8821 catcgaactg attatgcata tagtacaaac gaccaatgcg gaatttaatt tccggagctt
     8881 tttcggattt tggaaacttg gtaatatacc attgactggc tttaatgaag cgttcaactt
     8941 ttggatcaag agggagcggc tccaaagaat tagccacccg cttttgcaac tcctcatcgg
     9001 aaggaacgcc tttctccaaa gccaaaatta aattggctgc cgccttatcc ataaacttgt
     9061 tctgtggagc gttgtcgacc acccatttgt attgaaccgc agcttcatcg tagcgtttca
     9121 tatcatagag caattctgcg aaatagaaat gcatatctgc cgcaatcggc gtattgccaa
     9181 attcttcaag ataaaagcca tagccttcat aagccgtctt catcggtaaa gcggcgtgtg
     9241 aattctgagc tgtttgatgt tgttgcatca cgtagttacg taacgtggtt tcacgcaact
     9301 tgttggaggt ctccatcagt tctttattcg ctttattcgc ttcgtaccag ggagtatttg
     9361 ccgcgaagtt tttaatccat atgtagagct cttcacggaa tttcggagag tttttagcat
     9421 agaaataatt ttgcacgatc tgatattgcc aatcgaaggc tcgtggactc tctggtcttt
     9481 cgcctatcaa catcttaaac acatcacggg catcatcttt accaccctta tcagagtagt
     9541 aataagccaa cttctcaaga tagggaacga tatccccctc cggcaacaga ccccggaaat
     9601 aggcaatcgc gtcggaggct ggtcgagctt cggcgtagaa gatcacgatg tcacgaagag
     9661 cttcttgttc aaggcgattt ttattaacca cacgacgact ctgagaagtg gcttcagatt
     9721 cgtacttggc ggttttaatg atgtactcaa gataagacaa agcctgggcg ctttttccga
     9781 gacgaaataa acaccaggac cctttataaa gagcgaaagt atttaaacga tgttttttgt
     9841 ctttaataac ataagtatat tctttatatg catccgccca tttttcattc tcgaagtagt
     9901 attctcctaa agaaaaatga gactcgccca caaagggact ttgcggaaat tgtttattca
     9961 agcgaatata gtactcagcg cctttatggg tatcacccat ttcgaaataa ttataaccta
    10021 agaagaaaag tgcctggtcc atcttttcat ctttagggaa atcacgttca aaccattcat
    10081 acaattgaat ggctctctta ttaaaatcct ttgcctctgc tgtattcaaa accggtttaa
    10141 cttttgtttt accagctttg aaatccgcta aggcctgatc ataagcatct tgacggcggg
    10201 aatcgacgat tcccgctttt tccacataca gttccgcaag acgcagccat aattcaccgc
    10261 gattcgggct gtttttaaat ctttgtgtga gcttataaag ttcctggacc tgctgatcca
    10321 aaacacgttc atattccgcc tttgttccgc tgccacgatt gagcaaaatt tctgaggaac
    10381 ggggaggttt caccgactcc atattgtatt ggttctgact tggcttaaag ttatagccaa
    10441 actccggaag agctgtatta cccttatgca attggatgtt gccgccacga ctttcctggc
    10501 taatttgact cagtaattga ccaactgttt tctttccacc tgttgccgtc gtagtggtgc
    10561 tctgcttctt attgagggcc actgcatcca caccataagt gacaacgcca gtgaggaagc
    10621 tcaaaatcaa ggatgaattt aaaaggcggt tttttttatt tatcataggt tactcgcaac
    10681 tctgtttgcc taaatagtga tagtttccga cttcatccaa ccaatattcc ccttggaagg
    10741 gataatactc atacccattc tgtacgtaaa aagtacgatc gatcttctcg tcaatttgct
    10801 cagccgcgag atcttttccg gccaatttca ttttcaagcc ctctttttta ccgttgatca
    10861 tttcgtaacg gataaagccc gcttgctcat aaagatcgcg aagttcaaca cgcatattca
    10921 acatatgggc tttgaccata tcgcccaccg aaagtttcgc attccgttgg cgattgttca
    10981 ctactttcaa agtgtattgc ccaacaggag tatttttgaa tccaggattt gcatcaatcc
    11041 gggctttttc atcgttaaga cgtttgatat aataaagcga ccgtttcaca tttccttctt
    11101 cagaaatatt ttttacgacg ttataaggaa gtcgcaagtt aaccgcttta tcagactttt
    11161 tgatgagctg tcctttttca atctcagcat aatactccat ggcatccgtg ttgattcgta
    11221 taaagtcgcc aatcttggca cgaataggac cataggtatt ttcaaatagg cttaaaacct
    11281 tttccatctc atcatattta cagatataga gataaacgat ggagcgcaga agcaaggact
    11341 ccggaatata aaagtcatca tagtaactgg aatgcaatga ctgaaagtta cttaacacgg
    11401 aacgaaaacg agccgcacgc aacatggccc aagaggattc aaacaaagca tcatgccata
    11461 agaaatggtc gcggggaact tgcgcataag cctcaatgga agcatcccaa tcttgttttt
    11521 gataaagagc ccgtgcaata gccacctgag cagccacacg attcgtatcc gtcactggag
    11581 ccccatcccg tactgtcatc aatgtctgga aagtttgaag tgcagtatca atctgattac
    11641 cttcaagctc cgccaagcct cggttaaaga gagcttggtt atagtagcgg cttccctgac
    11701 ttaccttcga aaacaactca gaagcctttg gaaactcgcg ggctttcatt tttatctccc
    11761 cgatacggaa gtaaagcata tcccgattcg ttgaggggaa gtcgttcaaa tcgagtttag
    11821 aaaccgcgta gttcagcaaa gtatcatcac ccaagttgtc ggccacgatg gaaagcttct
    11881 caatcgcatt cttcacgtat ttgttattac ccatacggat aacatccacg aattgaaaag
    11941 cggcaacttg atttaatttc atttccatca gcattaaacc cagaacatat ttcaattggg
    12001 gacgctccgc ttggagctcc gctcggcgag ccaattgaaa caaggcatta gaggcttgtt
    12061 catactggct attttttgcc aaagccaagg cttgattcac ctgttgcctc aacggagaat
    12121 tctttactgc cgaagcagcg accccttgag atttcaatct cttctgagcc gcatacgccg
    12181 cggggaaaag aaaacttatg gttaagaaag ttgtgataaa ttttctcatc tgtaagtagc
    12241 ctccggatag aagaaactca taccgacagt cagaagcaaa tcactaaagc tttgcatggt
    12301 tccatctgtt gatttcgctg aatagttcat catgcttaga tcccagttca ttgaaaaacg
    12361 cttggacaga gcaaagtttt gaccggtgcc gaaatgaaga gtgaaaccac tgccaccggt
    12421 actcacgctc gtgtttccgc caccaaccgc gaaataatgt tcaaagggaa taatttttcg
    12481 attgagccaa ctcattttac cgtaaaaagg cgtgtacttg gcatcaaccc cgatgtaatt
    12541 ttttggaaac ccgaaactgt cggcactgac tccattgttg tcgtggagtt cttgcgctga
    12601 agttctttgt gaactcgtca ttgaggtata agtcccctca agcccccagg cttccgtgaa
    12661 atagtaggcc gctttcagtg aaaccccggc ggttaagaac caaggattat tggtgatcgt
    12721 ggaaaaggcg cccgtaaatt gcatgcgtcc tgttttcggc aaaaagcgct tttgaataac
    12781 ggagacttca ctgaatggct gaagttttgt taagcctgaa aaatccgtca gtttattact
    12841 ttgggcttcc agtttatctt cattggcctc aagcttttga ttttgcatgc ttggagctgc
    12901 cgtcggcggc ggacgcggag ctgtcttatc aagctccatc tcaatgacat tcaagtcatc
    12961 gccaatggct gaactcgagt cttgttgcgc ttttgcaaga ggcatcatca gcagcacacc
    13021 cacagaggcc atcaaaagta ttctacattg agtgttacag taagttttaa agtgtgtttg
    13081 catcgattcc atccttaaaa catgtaagac acaccaacat ttaaattggt ggtgtaagtg
    13141 agtcgttctg aaaactgatc aaagggcgga gctttcttaa ctccatcagt gggatccaag
    13201 gtatagggat tgcccgtgct gctataaaac ggaatgggtg cattgttagc gaacatgcgc
    13261 aaatcccaac gcaaactaaa ttgcttggac aaatgatact tttgtcccaa acccagcgcg
    13321 attcccagat aagatttatt ttggtacttc acaaaacctg cggcggccga accaaatgtc
    13381 gttaagttaa agaccaggct tttacttaaa ctcatttttc cgtaaaacgc tttaaagtta
    13441 taatccacca taaaagtaga ttgaggagcg ggagcccgag tgaagtctag atgaatatgc
    13501 gaacctgccg ttgagcccgg tacatccgtc aattgctgac catatttact aagccccgcg
    13561 gcattggact caaagaaggc tccaatagca tgattttcat tcacgtgata ataaccagcc
    13621 aagccgtatt tgctcacgtt aaagataggt tccgttagag cgtttccata gtagccgccg
    13681 agttcaaaac gacctgcggt tataatattt ctgtttttca cgctcacagg tttatcaaaa
    13741 actggataaa ctgactctgt agcaagttct tccgttggaa gttccaccac ttctgttgcg
    13801 aatgcgacac tctgtaaaaa gaacgccact aagaaaaagt ttaagaaaaa acttaggaaa
    13861 aaactcaatc ctttgagcgc attcttaatc atttatgacc gtcctgatga tgaaaaggct
    13921 ccgtgtttaa aaatcaaatc acaaacctcg cgaacggcac cgttgccgcc aggtctttta
    13981 gttacatatc gtgcgacttc aaggacttcc tcgaccgctt caggaactgt gacgccaaaa
    14041 gccacttctt gaagcagagg gatgtcaaaa atgtcgtctc cgatgtacgc catctccccc
    14101 acatgaaacc ctgatacttt ttgtaattct aaaaatgaag gaactttatc ggcagcgccc
    14161 tcatagaaaa attggatgcc tagatttttt acgcgttggc ggatgtcttc cgctttactt
    14221 ccagtgatca ctgcaagttt ataaccagat tcaataagac gttttatacc cacaccatcg
    14281 cgtatggagt agaaacgctt ccattcccca tcggaatcca tccaaagcct acaatctgtc
    14341 aacacaccgt ccacatccag gacaagcatt ttgacattgc gaaggcttag gttaagtttt
    14401 attgattctg gtcccattca aacattgtga aaaaaattca ccggctcggc aaggttatca
    14461 atgacttaac tcgactttcg tcaaattaat gtttagcgga tttttgttcg caaaaggtcc
    14521 tgaaagttca acattcccac aggttttttt ggatgatcgc tgagcttatc aaatacaaaa
    14581 agtatctgaa ttcggaattg ttccattaaa aacaaagctt tttccgcaag ctcattggca
    14641 tcaactccgc gcggggtggt cgtcatgagg tctttggcgg ttccggtgag tggatcagga
    14701 cttttatcga gtcgacgacg aatatcccca tcggtgatga ctccgaggag gtctccgttt
    14761 gcatcaagaa cccccgccgt gccacgaaca tttttatgag tcatgatgga aaataattct
    14821 cgcatcgggg tatccagccc gaccaaaggc aaggcatcac ctgtatgcat cacatcttta
    14881 acccgggtta aaagtctgta tcccaagctt ccgccgggat gaaattcggc aaattcttca
    14941 ggagaaaaac ctttttcatt caacacagcc atcgccatgg catcacccat cgccaaagaa
    15001 gccgtgctac ttgctgtggg agctaaattc aagggacaag cttcagcgga aactgaaaca
    15061 ttcaaaaccg ctttggccgc cttcgccaaa gtcgaattaa tatttccggt gatcgcaatc
    15121 acttgaatat ctttccggag agcaaaagcc agaataggag ccaactcggg agtttctccg
    15181 ccgtaactta aagccaaaat cacatcttcc gactcaacaa tccctaagtc tccatgcgca
    15241 ctctcggcag ggtgtaagaa aacagaagga gttcccgtcg aagaaaaagt gctcgcaagt
    15301 ttacgcgcaa tttgtccgga ctttccgata ccagtaagaa tcaactttcc tttgctggct
    15361 ttaataattt gcacggcttg aacaaaacca tcgctcaaac gctctttcaa agccaaaatc
    15421 gcattggcct cgatgtctaa aacttgccta ccttgaataa tgattttggt actcactcag
    15481 aacctctttt taaagccgct ttcacaaaat gcacaaagag cggatgcggg gccaaaggct
    15541 tcgacttaaa ctcaggatga aattgcacgc cgataaacca tggatgatcc actagctcaa
    15601 cgatctcaac taaatttctt tctttgcaaa cgccagtggc attcatgcca tttttttcaa
    15661 ataaagcacg atatttatta ttgtattcat agcggtgacg atgacgttca tgaatcaaag
    15721 ggcttttata gacattataa acgtgacttc ccttggtcag tgcgcaagaa aaggagccca
    15781 atctcatcgt gcccccttta tttactgaac ctctttgttc aatcatcgtg tcgataacat
    15841 agttacgagt tttgctgtca acgaagaact catggcttgt agcatcttgg atgccgcaca
    15901 catttcgcgc aaactcgatc gaagccaatt gcataccaaa acaaatcccg aaaaatggaa
    15961 tttggcgctc acgagcaaat ttgatggctg aaatttttcc ttccacacca cgggccccaa
    16021 agcctccggg aaccaaaatc ccttccacat ctccaagata ttgagccaca tttttatccg
    16081 tgagtttctc tgaatccaca tagacaatat tcactttgga attattagca atacctgcat
    16141 ggacgatggc ctcgttgaga gatttatagg attccttcaa atccacatat tttcccacca
    16201 ctccgatggt cacactctgt tttggattct gtaaaacttt aacgatcttt ttccagccac
    16261 tgaggtccat acggtgagat tggagctgca gtttttcaac aatgagttca tccaggcctt
    16321 ccttatgaag agccaaagga acttcataaa taaagctaga gtcctgggcc gcgataacat
    16381 ttt
//
LOCUS       DATBTE010000216        18393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1326300, whole genome shotgun sequence.
ACCESSION   DATBTE010000216 DATBTE010000000
VERSION     DATBTE010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 18393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18393
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326300"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VIG33_07925"
     CDS             <1..768
                     /locus_tag="VIG33_07925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HEY8270804.1"
                     /translation="PREILTAELQPGTYYFRVKNRSKNFDNTDRLRITVDGENITIPH
                     HSADESLLNPGDNSGVITVGAFDSDRSSISRSLNKPDIMAPSSMTLANGAEFRGSSNA
                     TAIVAAGVALVLSQNGGATKSQVLGRVSKNFNWDQGALSLNYLRFMPTAGNCFNSREW
                     NEAPDYIKQTLALGGALVETTLQYRVMVPFDPILLAIHQQRQYLDDMVIALPTGGFQV
                     VRRLSLIPQGAIELFQRPLEAGLCHTPLKRAGKIFLL"
     gene            838..1665
                     /locus_tag="VIG33_07930"
     CDS             838..1665
                     /locus_tag="VIG33_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270805.1"
                     /translation="MAKLLFSFLFSFLLSLSSGAVGPQCDFVFTPPEALTFGIEIEKQ
                     DPQEFMLTSYYNLMRRGQLEESTQIIKNISKNGIEALRDKDISTLKSTRKYVTILRSA
                     WHLSHKNHESPKSLERFAKNLGHLNDYIAAGKEKEAQREAHLVKKDIKNLDVEKLETL
                     MKPATQKSARSYLKTLRKEAREILKEKTVSLKNYHEVRKILKEFMTYYYLRDELNNEV
                     PGKEYQYLFHLNDELGLLNDQFTYQIINGLAHRKKTEIEFPADLKSKVHYFLENSSI"
     gene            complement(1706..4846)
                     /locus_tag="VIG33_07935"
     CDS             complement(1706..4846)
                     /locus_tag="VIG33_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8270806.1"
                     /translation="MKLSDLSIRNPVFAWMLMFGLIGFGLISFSRMGLSQLPDVDFPT
                     VSVSLTMAGAAPEVMETQVVDLIEGALMTVEGVQKITSTSKSGAATVTAEFSLNRNID
                     AALQDVQAKVAATQKRLPSELDAPTISKTNSDDQPIMFLALTYDKNDPEFMMKYTKDY
                     LQDRLTTVEGVGDLMLAGFTAPVMRVHVRPKDLTRYNISVNDVMEAIKNEHSELPGGF
                     LENSTKYFNVRTMGEAKSVEEFKSIIIASRGGQAVQDSSNMVRLSQVADISMGLDEIR
                     RIARLNGKSALGLGIRKQRGTNAVAVARGVKARMAELEKQLPEGMHLVVNFDSTQFIE
                     NSVHELNKHLVLAVLLTSLVCWAFLGSWSATFNVLLSIPTSLLGSFIGLYFLGYTLNT
                     FTLLGLTLAIGIVVDDAIMVLENIFRYNEQGHGRIESAILGSREIAFAAIAASVAVIA
                     IFLPVVFMKGIIGKFFMQFGITISLAVLLSLLESLTITPMRCASFVHHGARATKIGRS
                     FEMAMEKLRSGYEKSLHMILRHPWMVLGASVIFVAASFTSIRFINKEMAPAQDMSMFN
                     GRLMLPVGTSLAHTNEQVQKAEQWLLSRKEVERVYVAVGGFGGGGSSDANSAHILVSL
                     KPKGRRGIDEKAGHELSQVEFMQVARTAIAKIPDVRPVFMDMSMRGLSSGRGFPVEFT
                     VLGSDWDQLANMAKSIMEEMKKTGTVVDVDSDYLLGMPEIQIKPDRVAAAQRGVRVAN
                     IGNSVNALIGGVRVGEYPEGGHRYDIKVKLDDDNVTEEKIRTLMISNARGNLVPMSQV
                     TKTETNASLQAISRANRQRAITITANLVPGSSQQVAMGKVDEIAKKILKPGYMIAQGG
                     NAKTFQESFQSLMFALILGLFVAYMVLASQFNSFLDPLTILTALPFSFSGAFFALLLM
                     NQSINMYSMIGLLLLMGIVKKNSILLIEFTNTLRDRGVQTARQALIEACPVRLRPIIM
                     TSAATVAAAIPSALAVGEGSETMKPMAVCLIGGVIVSTVLTLFVVPCFYLVIDKFRTR
                     DEVRTKTKQAFATVGD"
     gene            complement(5053..5775)
                     /locus_tag="VIG33_07940"
     CDS             complement(5053..5775)
                     /locus_tag="VIG33_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8270807.1"
                     /translation="MQTQTVLNAGSVGVIGNTETSTVIPAASELPYEIIQLKEGDILF
                     KEGDMPRGLFYVHSGSVKVVVNRNMTRGRTASPEYVTKLVSPGEFFGYKSLVRGSPSS
                     SMAKAIRPTTVWLYPKEIVMGGLQHASPLLKMLLQQAVSDIESYEITNQLHYLASVQE
                     RIAYQLVVLADKFGVQTPNGISLNLKLTRNEFAQLASTINESLSRHLTEFKNEGLIDL
                     NGKEIIIKDMKTLMTKSGNFKI"
     gene            6052..7056
                     /locus_tag="VIG33_07945"
     CDS             6052..7056
                     /locus_tag="VIG33_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8270808.1"
                     /translation="MLKNFIFSMTILICGSASFAISSTAPLKVGLVLDKGGKDDKSFN
                     SAAYLGASKAEKELKIELKYVEATDTNALETLHRAFARKDFDLIIGVGFAQAEAVKKV
                     AAQFPKIKFALVDGEITAPNVRSLLFEDHEGSFLVGSAAALKSKTGKIGFVGGMDIPL
                     IRRFRLGYEAGAKQVNPKAQIITNFIGVTGEAWNNPAKAKELALAQISQGADVIFVAA
                     GASGSGVFDAVEEKKKLAIGVDSNQNWLKPGFILTSMLKRVDSAVYNTIQDTQSGKYT
                     AGAVHYGLKDKGVDYAMDNFNEKLLTPDLTAKLEGYKTQIIAGKIKVPDFYKQGKSSP
                     "
     gene            7053..8492
                     /locus_tag="VIG33_07950"
     CDS             7053..8492
                     /locus_tag="VIG33_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8270809.1"
                     /translation="MTRTLSPAVEFMGIHKSFGEFHAAKDLNFQVAAGEIHAIVGENG
                     AGKSTSMNILFGLYKPEAGTLKIHGKEVCFNSPMEAMNLGVGMVHQHFMLAQPLSALD
                     NILLFQKNPKALQRLNRKEHQARLQKLAAEYQFNIEWETPVQNLSVGTQQRIEILKIL
                     SQDSRIIILDEPTAVLTPQEIEDLFKNLKKLKAEGRTILIITHKLKEVMAIADAVTIL
                     RNGKVIANKKISETSVEDLAENMVGRKIKNLALSERRPFANQDLVMKIQDMNIDLQVR
                     KGEIVGVAGVEGNGQDALIQASLNPRSLKMCALRVAAFPEDRTRFGLLKERPLHENFI
                     LGYHNSPEYSRYGFLRFKNIFAKTKAAMEKYDVRPRDLQKPIGQLSGGNQQKLVVARE
                     LQNNPDFILAAKPTRGVDIGAIEFIHEQLLAARNRGAGILLVSTELEELMTLSDQIVV
                     LFKGQIVAKFLRSNNYNEIEIGAAMGGRT"
     gene            8489..9532
                     /locus_tag="VIG33_07955"
     CDS             8489..9532
                     /locus_tag="VIG33_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8270810.1"
                     /translation="MKKTFAGFFLGLTLALSLTLLAGENPFHIGGILFKSAFGSVYDL
                     GLTLFYTTPLIFCGLSVALAFHAGLFNIGGEGQLTMATLTAAAIGVFLPNIPFPLAPL
                     IALGGALLMGAFWGFIAGWLKAVRGSHEVIVTIMLNFIAAGLSSWFTLKVIPNPASQN
                     PETAPVGAGYLLKDYDLIAKSFPDTPVSMALVVAILVAFAVWLLLWKTSFGYELRAVG
                     YNPEAANRAGISEKKIKIWAMCLAGALAGFVALGEVMGGSGQFKIGFSADYGFIGIAV
                     ALLAQNNPLGVIFAALLMAALHKGAADLDLETTTITRDFSKIIQSLIILGVASHGLWV
                     WRAKKRLKGNRSE"
     gene            9477..10418
                     /locus_tag="VIG33_07960"
     CDS             9477..10418
                     /locus_tag="VIG33_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8270811.1"
                     /translation="MAFGFGARKSVLKEIAVNESLLVVATAFFSLLLAGLRMATPMVF
                     ASMGGMMSERSGVVNIALEGFMLTGAFTASVAAYFMHSAWMGWAFAIIAGCFIGALYA
                     FFVIELKADQIVTGTALNILTVGAMPFLTKILFSSTGSTPSLPLADRFSFEPVVLAFT
                     LVGVLTYWFYQTHSGLWLQFAGEHPAALSAAGVNVRKVRWFGVIASGAFAAMGGASLS
                     TFLASSYSPLMTGGRGYMALAALIFGRWKPLPTLAACLFFGITDALQIRLQGVHLNGI
                     EIPVQFIQILPYIVTIIALAGFFGKSEAPRSLGKVLE"
     gene            10415..11506
                     /locus_tag="VIG33_07965"
     CDS             10415..11506
                     /locus_tag="VIG33_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270812.1"
                     /translation="MKMLTHLLTVFLVLVNILSQANAWGGRGHAAICEAAVFLVKEPG
                     LKEYLKNKPQMMGHLCNMPDFFWRSFGADATKLGNPTHFIDVEVTGLKVPEVPLDFKQ
                     IVDTYTGKPNHFKAGATLHNVPSEFGSVWWRADQFYRRFLSFTKELATLEAPKTSKEA
                     TDDQLPYNKTVYEMSVSLGLMGHFVGDASQPLHNTADYDGYAAGHGGLHAYYEDQVVS
                     EFDGDLSSRVLKEARNMKNPLFLKPKTAIEKMKALSESSMDELKLIFKADPVKKPSTV
                     VSERGMEIKKAAERESAEIGYKKFNKIIVTELARSSLLLAKLWDDAYVEAGKPMLASS
                     KIYKYPLTVDFIAPDYFSKEEAPKTEDKK"
     gene            complement(11527..12051)
                     /locus_tag="VIG33_07970"
     CDS             complement(11527..12051)
                     /locus_tag="VIG33_07970"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HEY8270813.1"
                     /translation="MKLLNEKTLKKIIERVSEDLVGEWIVIGGTVLPLVGIDYRVTMD
                     VDIIPFGGTDNQSQLALMNIAADLNLPVETINQAGGFFLQKVPGWQQKVVLIRKGTKS
                     SVYRPNLELYFILKLARLSISDMQDCLEYYKWCNKNEEIHPAQIANLVQKQLKKENSK
                     EKKDRLEKLLKAIK"
     gene            complement(12048..12584)
                     /locus_tag="VIG33_07975"
     CDS             complement(12048..12584)
                     /locus_tag="VIG33_07975"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8270814.1"
                     /translation="MLKTNEIIRIKRNDLGLSQSQLAHEAGVSLPTIQNIEAGKANLS
                     QSSLEQILEVLGLKLQVVAVGLDWNKVVQLGVPLTGVNEMTSDFPVDIKNFVSRLDEF
                     LKTLSLKKEEMLKSSRNWEAFTAFLWALDTHYGKILRRLRYVKKAKFILSQNLEIFEL
                     GRLIKLRRIALANVGSFL"
     gene            complement(12737..16813)
                     /locus_tag="VIG33_07980"
     CDS             complement(12737..16813)
                     /locus_tag="VIG33_07980"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8270815.1"
                     /translation="MLEKKNIVTFKSFKSPSASLSSDLGISRALLAEAQDYISDDALK
                     DYKVSLDTISGLKAEFIFWNYGDGVDPRVTIQEKTPRGPLDLHCSQCTSPTLPNSLCL
                     HQAAGLQLCYESLAGSEDYDYYDDELSQVEGFLKEFNDAFENQEKRLQDQRKDFRIPK
                     DVQLKSISLKWFLPEKEASLIFKELREAPQADSFNFEIYKPAKIKDRHLPEIFREIDF
                     AKLSRIFYRGSEELREVQRIAGLFQYHFSNGVQVNSQQILLHPLGSLVPGHLRPRGMD
                     STNVDLDLGPWKNVTQTHVQRFYKGHELIRTERTMQALLRRIADEVNGGKIPFYIQDG
                     EQPTRVTPVKKIIGDLGENFVWSVTCQGSPLQATLTLKGEETSSSWIRFKEGAVDLSN
                     QYLLIYPLEDETDNLRYLLGNYGMCERETEQSTDQSELIQLKISGQESIFHGLRMLRE
                     RKAVVQFSGGTHEISQARLISEIHLNRSGYFQLRHKVIEKDFPEISFQGLSREVCQIL
                     IFLRSGISSFVGYEAKDLAARTQPKRDWDMKLLKHLGIFQYLVLELCSVAVEGCLTDG
                     TAVNNPTKIWPCLSGKIGSLLKITQDTGADLDFPLEKLCSVKVLEHFRSFISWFFETL
                     EVPTTTYAHQGEFVFAGLAKKDIQVLYILLKEQALLTKGACFQKARSSFTTKISEQIE
                     ENLPSLLLDGVSTSAPVQGVFRVPVAEKSVPSFQETMNVLAPLNEKGFQFYLDGSPLQ
                     GLADGDFSIEFTLNNTGVTDWFELNPKFFLKGKELAAGAEVQLGTGGVIEYQGRLYLV
                     PRKQIPSLSHLEGFWQRLQQGRKKNGSLSSFGAKVLPMPRSQMLELLALRASGYPVQG
                     GKEWEDLCTFYDSLGTPRKKSIDLSSSIRAELKPYQKEGVQWLHDLYCLKLGALLGDD
                     MGLGKTLQTLAFLETLRVQNKMGTTLIVVPPSLIYNWKSEIEKFVPDLPVRIFTTKER
                     ESLTALILAKTPLVVITTYGLLVENDVFFSAQNWNILIFDEAQNLKNITAKRTSAARS
                     LKANFRICITGTPMENHYGEFYSLMDLVAPGGLGEIDEFRQRYVIPSVVLAEDIHHLK
                     LKVKPLLLRRTKKEILFQLPEKQETKVSIAFERKQKKLYRDIAISYNEKIQAAIQTSG
                     ESKCQLQMLTALLRLRQACSDPSALPDVQYTQTPPKLETLLESLKEIVEANESALVFT
                     QFLPTLMRTQQELEKAGIAVYTIHGGVSATQRQKILKEFDQHPRGAVLLMTLKTGGVG
                     LNLTKASYVFHLEPWWNPAVENQATDRAHRMGQQKSVQVFRYIMHESVEEKIDLLKGR
                     KDQQFKSLFSDVENTSELEGTPHRGLTKEDFALLLGGY"
     gene            complement(16900..17574)
                     /locus_tag="VIG33_07985"
     CDS             complement(16900..17574)
                     /locus_tag="VIG33_07985"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEY8270816.1"
                     /translation="MGLFGKKMGLEMAVPMLLDMGGVNAYLIERYDRKKVRNQITRLH
                     QEDFCQALAYSHKVKYEKDGGPGFQECYQCAEELSSNLPEDLERLVKWLIFNVIVGNC
                     DAHAKNISFLMVEPNEWRVSPHYDIVSTKIYPNLSTKLAMTIGGSSDSGTITGSHWTR
                     LAKDIKVGTKWLHLTVQEMAEKSVGIFKETCHEFSEAYGKSSVLSELEKVILSQQRRL
                     LSQLKK"
     gene            complement(17588..17836)
                     /locus_tag="VIG33_07990"
     CDS             complement(17588..17836)
                     /locus_tag="VIG33_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HEY8270817.1"
                     /translation="MMNSKLNLYCNETLIGSLWEDSQHRIEFAYASIWLKSIKKYSIS
                     HSMPLREEAYSREAQNYFANLLPEGDIRIAVAAKLGIS"
     gene            complement(17823..18068)
                     /locus_tag="VIG33_07995"
     CDS             complement(17823..18068)
                     /locus_tag="VIG33_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8270818.1"
                     /translation="MQVNVKKVSDISKLVRELRHQKGLSQEKASALAGVGRRFFSELE
                     RGEKESLDLGKVLQVLRKFGVKLTLEARTGKDPHDEL"
BASE COUNT         4926 a         4405 c         3998 g         5064 t
ORIGIN      
        1 ccgcgtgaaa tcctgaccgc agaattgcaa cctggaacct attatttccg tgtgaaaaat
       61 cgcagcaaga actttgataa cacggatcgc ctccgcatca ctgtggatgg tgaaaacatc
      121 acgatccccc atcactccgc agatgaatcc ctcctcaacc ccggcgacaa ttctggagtg
      181 atcacggtcg gcgcctttga ttcggatcgc agttccattt ctcgaagcct gaataaacct
      241 gatatcatgg ctccttcttc gatgactctc gcgaacggcg ctgaatttcg tggaagttcc
      301 aatgccaccg cgatcgtggc tgcaggtgta gctctcgtgc tatcgcaaaa tgggggagct
      361 actaaaagcc aggtattagg aagggtttct aaaaacttca actgggacca gggcgctttg
      421 agtttaaact atcttcgttt catgccaacg gcgggaaatt gttttaattc ccgggagtgg
      481 aatgaagccc ctgattatat caaacagact ctggcgttgg gtggtgctct cgtcgaaacc
      541 actttgcagt atcgggtgat ggtgcctttt gatccgatcc tcctggcgat ccatcaacaa
      601 cgccagtacc ttgatgatat ggtgattgct ttgcccacgg gcggttttca agtggtgcga
      661 cgcttaagcc tcatccctca gggagctatt gaacttttcc aacgtcccct cgaagccggt
      721 ctctgccata cgcccttgaa acgggcggga aaaatctttt tgctttagcc ttaccaattg
      781 aatccgtctc aaaatgagac aataagtaca tgcacgattt ttgcatctta tatcggcatg
      841 gcaaagctgt tgttttcttt cctcttttct ttccttctga gtctgagttc tggggccgtc
      901 ggtcctcagt gcgatttcgt cttcactcca cccgaagctc tcacctttgg tatcgagatc
      961 gaaaagcagg atccgcaaga gtttatgtta acttcttatt ataatcttat gcgcagaggg
     1021 cagcttgaag agagtactca gattataaaa aatatctcaa aaaatgggat cgaagcgctt
     1081 cgcgataaag acatttcaac tcttaaaagc actcgcaaat atgtgacgat tctacgctca
     1141 gcttggcacc tcagccataa aaaccatgaa agccccaaat ccttggaacg atttgctaaa
     1201 aaccttggcc atttaaatga ttacatcgcc gcaggtaagg aaaaagaagc ccagagggag
     1261 gctcatctgg ttaaaaaaga tatcaagaat ttagatgttg aaaagctgga aacccttatg
     1321 aaacccgcga cgcagaagtc ggctcgttcc tatctgaaga ctcttcgaaa agaggcacga
     1381 gaaattttaa aagagaaaac agttagtctg aagaattacc atgaggtgag aaaaattctt
     1441 aaggagttta tgacttacta ttatctcaga gacgaattaa acaacgaggt tcctggcaaa
     1501 gaataccaat accttttcca cttaaatgat gaattgggtc tgctcaatga ccagttcacc
     1561 taccaaatta tcaatggctt ggcccataga aagaaaacag agatagagtt tccagcggat
     1621 ctaaaatcca aagtgcatta ctttctggaa aactcctcaa tttaacccct tcatcgcgac
     1681 gcatccgaaa aaggtacggc gtaccctaat ctcctactgt ggcgaaggct tgttttgttt
     1741 tggtgcgaac ttcgtcgcgg gttctgaatt tatctatgac cagataaaaa caaggaacta
     1801 caaacaaggt aaggactgtc gagacgatga ccccgccgat taaacagacc gccatgggtt
     1861 tcatggtctc ggaaccttcg cccacagcaa gggctgaagg aattgcggcg gcgaccgtgg
     1921 cggcggaagt cataatgatg ggtcttaaac gcacgggaca agcttctatt aaagcctgtc
     1981 gtgccgtctg caccccgcgg tcacggagcg tgttggtaaa ctcaatcaat aaaatggagt
     2041 tcttcttcac gatccccatc aaaagcaata agccaatcat ggaatacata ttgatggatt
     2101 gattcatcag cagaagtgca aagaaagcgc cgctgaaact aaaaggcaag gcggttagaa
     2161 tcgtcagggg atcaaggaag gagttgaact ggctagctaa caccatgtag gccacaaaaa
     2221 gccctaagat caaagcgaac attaaacttt ggaaagactc ctgaaatgtt ttcgcatttc
     2281 cgccttgggc gatcatataa cccggcttca gtattttctt cgcgatttca tcaactttgc
     2341 ccatagccac ttgctgagaa gagcccggaa ccaaattcgc tgtgatggta atggcacgtt
     2401 gtcgattggc tcttgaaata gcctgcagac tggcattagt ttcagtttta gtcacctgac
     2461 tcatcggcac taaatttccg cgagcattgc tgatcatgag cgttcgaatc ttttcttcag
     2521 tgacattgtc atcatcgagc ttcactttaa tatcgtaacg atgaccgccc tcaggatact
     2581 caccgactct cacaccaccg atcaaagcat tcaccgagtt gccgatgttc gccacgcgca
     2641 ccccgcgttg agccgccgcc acacgatcgg gcttaatctg aatttccggc attcctaaaa
     2701 gataatccga atccacatcc accacagttc ccgttttttt catttcctcc atgatggatt
     2761 tcgccatatt cgccaactga tcccaatcag atcccaacac cgtgaactcc actggaaaac
     2821 ctcttccgct ggaaagccca cgcatcgaca tatccataaa caccggcctg acatcaggaa
     2881 tctttgcaat cgcggtccgg gccacttgca taaactcgac ttgggaaagc tcatgacccg
     2941 ctttttcatc gatcccacgc cggcccttcg gtttcagact gacaagaata tgggcgctat
     3001 tggcgtctga cgatcctccg ccgccaaatc ctcccacggc gacataaact ctttcgactt
     3061 cttttcgaga cagcaaccac tgctcggctt tttgcacttg ttcattggtg tgagcaaggg
     3121 acgtacccac cggcaacatc aagcggccat tgaacatgct catatcctgt gccggggcca
     3181 tttctttatt gataaaacgg atcgaagtga aggaggccgc cacaaagata acagaagccc
     3241 ctaagaccat ccacgggtgt cgcaagatca tatggagaga tttctcatag ccacttcgca
     3301 gtttttccat tgccatttca aaagagcgac caatcttggt cgctcgcgcg ccatgatgaa
     3361 caaagctcgc acaacgcatc ggcgtgatgg tcaaagactc caacaatgac agcagcaccg
     3421 cgagggaaat cgtgatcccg aactgcataa aaaacttacc aatgatccct ttcataaaga
     3481 ccaccggtaa gaaaatcgca atcacagcca cagaggcggc aatggccgca aacgcaatct
     3541 ctcgtgaacc caaaattgct gactcaatcc ggccatgtcc ttgttcatta tatctaaaaa
     3601 tattttccag aaccatgatg gcatcatcga ccacgatccc gatcgctagt gtaagaccca
     3661 gaagagtgaa agtattcagc gtataaccca agaaatagag cccgatgaag gaccctaaaa
     3721 gagacgtcgg aatcgacagg agcacattga aagtggcgga ccagctgcct aaaaaggccc
     3781 aacaaaccaa agatgtcaaa agcaccgcca aaactaaatg cttgttcaat tcatgaacag
     3841 agttctcaat aaactgagtg ctatcgaagt tcacaaccaa atgcatgccc tctggcaatt
     3901 gcttttctaa ttccgccatt cgcgccttaa ctcctcgagc caccgccacg gcattggtgc
     3961 cgcgttgttt gcgaattccc aagcctagag cggacttccc attgagtctg gcaatgcggc
     4021 ggatttcgtc cagtcccatg ctgatatccg ccacctgaga aagccgaacc atgttgctag
     4081 aatcctgaac agcctgtcct ccgcgactgg cgataatgat ggatttgaat tcctcgactg
     4141 acttcgcttc ccccatggtg cgaacattga aatatttggt actgttttct aaaaatcccc
     4201 cgggcaattc cgaatgttca ttcttgatag cttccatgac atcgttcaca gaaatattat
     4261 accgagtgag atctttgggg cgtacatgaa ctcgcatcac cggagccgta aagcccgcca
     4321 acattaaatc acccacaccc tccacagtcg tcaaacgatc ctgcaagtaa tctttggtgt
     4381 atttcatcat aaactcggga tcattcttat cgtaagtcaa agccaagaac atgatcggct
     4441 gatcatccga atttgttttc gaaatcgtag gggcatctaa ctcagagggc agacgttttt
     4501 gagtggcggc gactttcgcc tggacatctt gaagagcggc gtcgatattg cgattcaaag
     4561 aaaactccgc agtcacagtg gctgcgcctg atttactcgt agaagtgatc ttttgcactc
     4621 cctcgacggt catcaaggcg ccttcgatca ggtccacgac ttgtgtttcc atcacctcgg
     4681 gggccgctcc tgccatcgtt aaagagacac tgacggttgg aaaatccaca tcgggcaatt
     4741 gactcaggcc catcctgcta aaggaaatta aaccaaaacc aattaaaccg aacatgagca
     4801 tccacgcgaa aacaggatta cgaatggata aatccgagag cttcatagtc accccaccag
     4861 ttctgctatt ccacgcctaa aaatgctatt aggcaattgt ttcaaagcag atgatcggcc
     4921 ttgcataata cttccggccg aaaaaaagcg gatttacatc cgcttttaaa gagagggact
     4981 taataagctt gcgataaaaa ctagactttt ggaaagtgat gcttttatgg ctaaattcta
     5041 ccctgtattt acctaaatct taaaattgcc tgatttagtc ataagggtct tcatgtcttt
     5101 gataataatt tctttgccgt tcaaatcaat caaaccttca tttttaaatt cagtcagatg
     5161 acgggacaga gattcattga tggtgcttgc cagttgcgcg aactcattac gggtgagttt
     5221 cagattcaaa gaaatgccgt tgggggtttg cacgccgaat ttatccgcca acaccactaa
     5281 ctgataagca atccgctctt gcacggaggc aagataatgc aattgattcg tgatctcata
     5341 actctcgata tcactgacgg cttgttgtag aagcattttc aataaaggcg aagcatgttg
     5401 aagaccgccc atgacgatct ctttaggata aagccagact gttgtcggac ggatggcttt
     5461 cgccatcgaa gaagagggcg agcctctgac cagggattta taaccaaaaa attccccggg
     5521 tgacaccagt ttagtgacat attcaggaga agctgtacga cccctggtca tattacgatt
     5581 caccaccacc ttcactgaac cgctgtggac ataaaaaagc cctcttggca tgtccccttc
     5641 cttaaagagg atgtcgcctt ccttcaactg aatgatttca tagggtaact ccgaagctgc
     5701 cggaatcact gtgcttgtct ctgtatttcc aatcactccg acggaacccg catttagaac
     5761 tgtctgtgtt tgcattgcaa acctccaatg cttgcattgt ttatacgagt gtttactgac
     5821 gtaaactgta aggcttattg gtatccccca ggttcgcaaa tctccaccta cgtggagcga
     5881 attttttgtt aactttcagg gataaatttg ccttgacccc ccaaggcatt gtggcctaaa
     5941 tttcaccgag tttggcaaac ttttataata aaatcaggaa gttaaccaag tttgattcat
     6001 aaacaattaa aaactcacaa ataactcact agtcatttaa ggaggtttat tatgttaaaa
     6061 aacttcattt tttcgatgac gattctaata tgtggcagcg cttccttcgc aatttcttcc
     6121 actgctcccc tcaaagtcgg gcttgtcctc gataaaggag gcaaagacga taagtctttt
     6181 aattccgctg cttatctagg agcttccaag gcagaaaaag aacttaagat tgaacttaaa
     6241 tatgttgaag ccactgatac gaatgctctg gaaactcttc accgtgcttt cgcccggaaa
     6301 gacttcgacc tcattattgg agttggtttt gcgcaagcag aagcagttaa aaaagtagcc
     6361 gcacaatttc cgaaaataaa atttgcgttg gtcgatgggg aaatcaccgc gccgaatgtc
     6421 cgctccttgc tttttgagga tcacgaaggc tcttttctcg tcggcagtgc tgccgcctta
     6481 aaatccaaaa ccggcaaaat tggttttgta ggaggaatgg atattcccct gatccgtcgc
     6541 tttcgattgg gttacgaggc gggagctaaa caggtgaatc ctaaagctca aatcattacc
     6601 aattttattg gcgtcacagg tgaagcctgg aacaaccccg ccaaagcaaa agagttggcg
     6661 ttggcccaga tctctcaagg agcggatgtg atctttgtcg ccgctggagc ctccggatca
     6721 ggagtgtttg atgcggttga agaaaaaaag aaactcgcta tcggcgttga ttcgaatcaa
     6781 aattggctga agccaggctt tatcttgacc agtatgttaa agcgcgtgga ttccgcagtt
     6841 tataatacca tccaagatac tcagagcgga aagtacactg ccggtgctgt ccactatgga
     6901 cttaaagaca aaggcgtcga ctatgcaatg gataacttta atgaaaaact tttaactcct
     6961 gatctgaccg caaaattaga gggatataaa acgcaaatca tcgcgggaaa aataaaagtt
     7021 cctgactttt acaaacaggg aaaaagcagt ccatgactag gaccctttcc cctgccgtgg
     7081 aattcatggg gattcataaa agcttcggcg aattccacgc cgcaaaggat ctcaattttc
     7141 aagtggccgc aggagaaatc catgccattg tcggtgaaaa tggagccggc aagtcgacct
     7201 ctatgaatat ccttttcgga ctctacaaac ctgaggcagg cacccttaaa atccatggta
     7261 aagaggtttg ttttaattca ccaatggagg ctatgaactt gggcgtcggc atggttcatc
     7321 agcacttcat gctcgctcaa cccttgagcg cccttgataa tattttgctc tttcaaaaga
     7381 atccaaaggc cttgcaaagg ctgaatcgta aagaacatca agcgcgtttg caaaagctcg
     7441 ccgctgaata tcagttcaat attgaatggg aaaccccggt gcaaaatctc tcggtgggca
     7501 cccagcaacg cattgaaatt ttgaaaattc tttctcagga ttctcggatt atcattctcg
     7561 atgaacccac ggctgttttg actcctcaag aaattgaaga tttatttaaa aacttgaaaa
     7621 aattgaaagc cgaaggccgc acgattttga tcatcactca taagttgaaa gaagtgatgg
     7681 ccatcgcgga tgcggtgacc attcttcgca atggcaaggt gattgccaat aaaaaaatat
     7741 ctgaaacctc cgtggaagat cttgcggaaa acatggtagg cagaaaaatt aaaaatctgg
     7801 ctctgagcga acgccgccct tttgccaacc aagacttggt catgaagatc caggatatga
     7861 atatcgactt acaggtgcgc aagggtgaaa ttgtcggtgt ggccggggtc gagggcaacg
     7921 gacaagatgc cttgatccag gcttcactca atcctcgctc gttgaaaatg tgcgccctca
     7981 gggttgccgc cttcccggaa gatcgcactc gttttggtct tctcaaggaa cgtccccttc
     8041 atgagaactt catcttggga tatcacaatt cccctgagta ctcacgctac gggtttttga
     8101 ggtttaaaaa catcttcgcc aaaaccaagg ccgcaatgga aaaatatgac gtgcggcctc
     8161 gcgatttaca aaaaccgatc ggccagcttt cgggcggaaa ccaacagaaa ctcgtcgtag
     8221 cccgtgagtt gcaaaacaat cctgatttta ttcttgccgc taaacccacc cggggcgttg
     8281 acattggggc catcgagttt attcacgagc agcttttggc cgcgcgcaat cggggcgctg
     8341 gcatcctctt ggtctctacg gagctcgagg aattgatgac cttgtccgac cagattgtgg
     8401 ttttgttcaa aggccaaatc gtggcgaaat ttttacgttc caataactat aatgaaattg
     8461 aaattggcgc ggccatggga ggccgtactt gaaaaaaacc ttcgcaggat ttttcttagg
     8521 actcactctt gctctgtctt tgactttgct cgcaggggaa aacccatttc atatcggtgg
     8581 catccttttt aaaagtgcgt ttggatccgt ttatgatttg ggtctgactt tgttctatac
     8641 cacgccgttg attttttgcg gcctttcggt cgctctggcc ttccatgcgg gattgtttaa
     8701 tatcggcggc gaaggtcaat tgacgatggc tacgctgact gccgccgcca tcggcgtgtt
     8761 tctgcccaat attccgtttc ctttggctcc gctgatagct cttggaggag cactcttaat
     8821 gggagctttt tggggcttta tcgctggctg gctcaaggct gttcgcggca gtcatgaagt
     8881 gatcgtgaca attatgttga acttcatcgc cgccggtctt tccagttggt ttactttaaa
     8941 agtgattccc aatcccgctt cacaaaatcc tgaaaccgct ccggtgggtg ctgggtacct
     9001 gttgaaagac tatgatctta ttgccaaatc atttcccgat acgccggtaa gtatggctct
     9061 ggtggtggcg attttggtgg ctttcgctgt atggctgctg ctctggaaaa ccagctttgg
     9121 ttatgagctt cgcgctgtcg gatataatcc cgaggccgca aatcgtgcgg ggatttctga
     9181 aaagaaaatt aaaatctggg ccatgtgttt agccggtgcc ttggcgggct ttgtcgctct
     9241 cggcgaagtg atgggaggca gtggtcaatt taaaattggt ttctctgcgg actatggctt
     9301 tattggtatc gccgtcgcct tgctcgcaca aaacaatcct ctcggggtga tcttcgccgc
     9361 tctcttaatg gcagcgctgc acaaaggagc cgcggattta gatcttgaga ccacgacgat
     9421 cactcgggat ttttccaaga ttattcaaag tttgattatc ttaggagtgg cctctcatgg
     9481 cctttgggtt tggcgcgcga aaaagcgtct taaaggaaat cgcagtgaat gagtctcttc
     9541 tagtggtagc caccgcattc ttctcccttt tattggcggg acttcgcatg gccacgccga
     9601 tggttttcgc ctccatgggc ggaatgatga gcgaacgctc gggcgtggtc aatatcgcac
     9661 tggaaggttt catgctcacg ggggctttta ccgcctcggt ggccgcatac tttatgcatt
     9721 ccgcgtggat gggttgggcc ttcgcaatca tcgcaggctg cttcatcggc gcactgtatg
     9781 cattttttgt gattgaatta aaggctgatc aaattgtcac gggaacggct cttaatattt
     9841 tgactgtggg agcgatgcct tttcttacta agattctttt cagctctacg ggttccactc
     9901 cgagtctgcc tttagccgat cgttttagtt ttgagcccgt ggtgttagcc tttactctgg
     9961 tcggcgtttt gacctattgg ttttatcaaa ctcattccgg gctgtggctg caatttgctg
    10021 gtgaacatcc tgcggccctt tcggcagcgg gtgtgaacgt tcgtaaggtg cgttggtttg
    10081 gcgtgatcgc cagcggtgct ttcgccgcga tgggaggcgc cagcctttcg actttcttgg
    10141 cttcttctta ttctccgctg atgacggggg gtcgcggcta tatggcccta gccgctttga
    10201 tctttggtcg ttggaaacca ttgccgacac tagcggcgtg tttattcttc ggaataaccg
    10261 atgctctgca aattcggctg caaggcgttc acctcaatgg cattgaaatt cctgttcagt
    10321 ttattcaaat tttaccttat atcgtaacca tcattgcgct tgctggtttc tttggaaaaa
    10381 gcgaagcccc tagatcctta ggaaaggtgt tagaatgaaa atgttaactc atctattgac
    10441 tgtcttcctt gttctcgtaa atattttgtc ccaagccaat gcctggggag gccgtggtca
    10501 cgctgccatt tgtgaagccg ctgtcttttt ggtcaaagaa ccgggcttaa aagaatattt
    10561 aaagaacaag ccccaaatga tgggtcatct ctgcaatatg cccgatttct tttggcgctc
    10621 ctttggcgct gacgcgacga aattgggcaa cccgactcat tttatcgatg tggaagtcac
    10681 gggtcttaag gttcctgaag ttcctctgga ttttaaacag atcgtggata cctacaccgg
    10741 caagcccaat catttcaaag ccggagccac tcttcacaat gttcccagcg aattcggttc
    10801 tgtatggtgg agagccgatc aattttatcg ccgcttcctg agtttcacta aagagctggc
    10861 gaccctggaa gcccctaaga cttccaaaga ggccacggac gatcaacttc cctacaacaa
    10921 aacagtctat gaaatgtccg tgagtctggg tttgatgggt cactttgtcg gagacgcttc
    10981 tcagccactt cacaataccg cggactatga tggttatgcc gcaggccacg gtggcttgca
    11041 tgcttactac gaagatcaag tggtcagcga attcgatggg gatctttctt ctcgtgtttt
    11101 aaaagaagct cgtaacatga aaaatcccct gttcttgaaa ccaaaaaccg cgatagagaa
    11161 aatgaaggca ttgagtgaat cgtccatgga cgagctcaag ctcatcttca aagccgatcc
    11221 tgttaagaag ccctcaaccg tggtcagcga acgaggcatg gagatcaaaa aagcggcgga
    11281 acgcgaaagt gctgaaatcg gctataaaaa gtttaacaaa atcattgtca ccgaactggc
    11341 ccgttccagt ttactgctgg ccaagttgtg ggacgacgct tatgtggagg cgggtaaacc
    11401 gatgttggct tccagcaaaa tctataaata tcctctgacg gtggacttta tcgctccgga
    11461 ctatttttct aaggaagagg ctccgaaaac ggaagataaa aagtaaccag gttcaattta
    11521 aggttcttat ttgatcgctt tgagcaattt ctcaaggcga tctttttttt cttttgagtt
    11581 ttctttcttc aactgttttt gcacgaggtt cgcaatttgc gcgggatgaa tctcttcatt
    11641 tttattgcac catttatagt actcaagaca atcctgcata tcggagatgc tgagtctcgc
    11701 cagttttagt ataaaataaa gttccagatt tggtcgatag acagagctct ttgttccttt
    11761 ccttatcaat actacttttt gctgccatcc aggaactttc tggagaaaaa atccaccggc
    11821 ttggttaatc gtttccacgg gaagattgag gtcagctgca atattcatta aggccaattg
    11881 gctctgattg tccgtccctc caaaaggtat tatatctacg tccatggtga ctcgatagtc
    11941 gattcctact aaaggtaaga cggtcccccc aataacaatc cattcgccga caaggtcttc
    12001 tgaaacccgc tctatgatct tttttaaagt cttttcgttc agtagtttca taaaaagctt
    12061 ccaacgttcg caagggcaat acgccttaac tttataagcc gtccaagttc aaaaatttct
    12121 aaattttggc taagaataaa cttggctttt ttaacatagc gaagacgtcg caagattttc
    12181 ccatagtgag tatccaaggc ccataaaaag gccgtaaatg cctcccagtt tctagagctt
    12241 tttaacattt cttctttttt caacgaaaga gttttcaaaa actcatctaa gcgacttaca
    12301 aagtttttaa tatcaacagg aaaatctgaa gtcatctcgt tgactcccgt caacgggaca
    12361 cctaattgta cgactttatt ccagtctaaa ccaacagcaa caacttgaag ttttaaaccg
    12421 agaacttcta agatctgttc taagctggac tgagaaagat tggcctttcc cgcttcaata
    12481 ttttgaattg ttggaagact gacccctgct tcgtgagcca actgactctg tgacaagcct
    12541 aagtcgttcc ttttgattct gataatttcg ttagttttta gcataatata ttatgcatat
    12601 tataatatat tcttctggtg aattacaata aaaatgcatt ttagtgaaaa ttaggacttt
    12661 attactaatt tatgcctata taacaggatt atattcctat cgatgggcac acccccttgc
    12721 ccaatatttg tcttggtcaa tatcctccga ggagtaaagc gaaatcctcc ttcgtgagcc
    12781 cgcgatgggg agttccctca agctcagagg tgttttccac atctgaaaat aaagatttaa
    12841 actgctgatc tttgcgccct ttcaacaggt caattttttc ctcgacggac tcgtgcatta
    12901 tatatctgaa aacctggact gatttttgct gtcccatacg atgtgcgcgg tcagtcgctt
    12961 gattttccac ggccggattc caccacggct caaggtgaaa gacatagctg gccttggtaa
    13021 ggtttaatcc gacacctccc gttttgaggg tcattaagag aacggcccct cgggggtgtt
    13081 gatcaaactc cttcaaaatt ttctgtcttt gagtggcact gactccgcca tgaattgtgt
    13141 agaccgcaat ccccgccttt tccagctctt gctgcgtgcg catcaaggtg ggcaaaaatt
    13201 gcgtaaaaac caacgcactt tcattggcct cgactatttc cttgagagac tccaataaag
    13261 tttccagctt aggcggcgtt tgagtgtatt ggacatcggg taaagccgaa ggatctgagc
    13321 aagcctgtcg caatcgcaat aaagctgtta acatctgaag ttggcatttt gattctccag
    13381 aggtttggat agcggcctga attttttcat tataggaaat cgcaatgtcc cggtagagtt
    13441 tcttttgctt gcgttcaaag gcaatgctca ccttggtttc ttgcttctca ggcaactgga
    13501 aaagaatctc ttttttcgtt cttctcaata gaagaggttt cactttcaac ttaagatggt
    13561 ggatgtcctc agctagaaca acgctcggaa taacataacg ctgtctgaac tcgtcgatct
    13621 cgcccaaacc tccaggcgca actaaatcca taagcgaata aaattcgccg taatgatttt
    13681 ccatcggagt acccgtgata caaattctaa agttagcctt gagggatctc gccgccgagg
    13741 tgcgttttgc tgtgatattt tttaaattct gagcttcatc aaaaatcagg atattccaat
    13801 tttgcgcact gaaaaaaaca tcattttcaa caagcagacc gtaggtcgta ataaccacga
    13861 gaggggtttt ggccaaaatc agagccgtca aactttcgcg ctctttagta gtgaaaatcc
    13921 tgactggtaa atctggaacg aacttttcta tttcactttt ccagttataa atcagcgatg
    13981 gcgggacaac gattaaagtc gttcccattt tattttgaac ccgtaaggtt tctaaaaatg
    14041 ccagggtttg taaggtcttg ccaaggccca tatcatcccc caacagcgcc cctaacttca
    14101 aacaatacaa atcatgaagc cactggaccc cttctttttg atagggcttt aactccgcac
    14161 gaatcgagga ggagagatct atggattttt ttctgggggt ccctagtgaa tcataaaaag
    14221 tgcaaaggtc ttcccactct tttcctcctt ggactggata gccagaggca cgtaaagcca
    14281 aaagttccag catttggctg cgaggcatcg gtaaaacttt ggctccgaaa gaagagaggg
    14341 acccgttctt tttccggcct tgttgaagcc tttgccaaaa accttctaag tgactcaggg
    14401 aagggatctg cttccttggc actagatata accgaccttg atactcgatc actccacccg
    14461 tccccagttg aacctctgcg cccgcagcca actctttacc cttcaagaaa aatttaggat
    14521 tgagttcgaa ccaatcggtg actcctgtat tgttcaaggt gaactcaatg gaaaaatcgc
    14581 catctgctaa accttgcaag ggacttccgt ccagataaaa ctgaaaacct ttttcgttta
    14641 agggcgccaa tacattcatg gtctcttgaa aagagggcac ggatttttcc gcgacaggaa
    14701 ctctgaaaac cccttggact ggtgcagaag tagaaacccc atccaataaa agagagggca
    14761 gattctcttc gatttgttca gaaatttttg tcgtaaagga agatctggcc ttttgaaaac
    14821 acgcgccctt ggtgagtagg gcttgttctt taagcagtat ataaaggacc tgaatgtctt
    14881 tctttgccag tcccgcaaaa acaaactctc cttggtgcgc ataggttgtc gttggtactt
    14941 ctaaagtttc aaaaaaccag cttataaaac ttcgaaagtg ctcaagcact tttaccgaac
    15001 acagtttttc cagagggaag tctaaatcag cgccagtatc ctgtgtgatc ttcaataagg
    15061 atcctatttt accagacaaa cagggccata ttttggtggg attgttcaca gctgtgccgt
    15121 cggttaaaca accttctaca gccacagaac aaagttccaa aacgaggtac tgaaaaatac
    15181 ctaaatgctt caaaagcttc atgtcccaat ctcttttggg ctgggttcgt gctgcgagat
    15241 ccttagcttc gtaaccaaca aaagaggaga ttccagatct taagaagatc agtatttgac
    15301 agacctcacg ggaaagcccc tgaaagctta tttctgggaa atccttttct atgaccttgt
    15361 gtcgcaattg aaaatatccg gaacgattga gatgaatttc cgaaataagt cgtgcttgac
    15421 tgatttcgtg agtgccaccc gaaaattgaa ctacggcctt acgctctctc aacatcctaa
    15481 gcccatgaaa aatactttcc tgcccagaaa tctttaactg gatcaattca ctctgatccg
    15541 tactctgctc ggtctcgcgc tcgcacatcc catagttccc aagaagatat ctcaaattat
    15601 cggtctcgtc ctcgagggga taaatcaaaa gatattgatt ggagagatcc accgccccct
    15661 ccttaaacct aatccaggat gaggaagttt cctcgccttt caaagtgagt gtggcttgca
    15721 gcggactgcc ttgacaggtc accgaccaca caaaattctc gcctaaatcc ccgatgattt
    15781 ttttaacagg cgtcacacgc gttggctgtt ccccatcttg tatataaaat ggaatcttac
    15841 cgccgttgac ctcatccgca atccgccgca aaagagcctg catcgtgcgt tcggttctta
    15901 tcaactcgtg ccctttgtaa aatctttgaa catgagtctg agtgacattt ttccaagggc
    15961 ccaaatccaa gtccacattc gtggaatcca taccccttgg gcgcaagtgg cctggcacta
    16021 aggatcccaa cggatgtaaa agtatttgtt ggctattgac ctgaactcca ttgctaaagt
    16081 ggtactgaaa aagtccggcg attcgctgga cttcccggag ttcttcagaa ccgcggtaaa
    16141 atatgcggga aagcttcgcg aaatctatct ctctaaatat ctcgggaagg tggcgatctt
    16201 tgatcttagc gggtttgtag atctcaaaat taaaactatc cgcttgaggg gcttcccgaa
    16261 gctctttgaa aatcaacgaa gcttcttttt caggaaggaa ccacttcagg ctgatggatt
    16321 ttaactggac atctttcgga atccgaaaat cttttctttg atcttgaagt cgtttctctt
    16381 ggttttcaaa ggcatcatta aactctttta agaagccctc gacctgcgag agttcgtcat
    16441 cataataatc ataatcctca ctccccgcca gactctcata gcagagctgc aaccccgcag
    16501 cctgatgtaa acaaagagaa ttaggtaagg ttggcgacgt acactgacta cagtgaaggt
    16561 ccagaggccc gcgaggagtc ttttcttgga tcgtaacccg aggatccact ccatctccgt
    16621 agttccaaaa aataaactct gctttcaacc cagaaatagt gtcgagactg actttgtaat
    16681 cctttaaagc atcatcagaa atgtaatcct gagcctccgc cagcaaagct cttgaaattc
    16741 cgagatcact tgagagggaa gcactcgggc ttttgaagga tttaaaagtg acgatgttct
    16801 ttttctctag cacgaaggcc cccttgcttt tcgaccctct gtttatgggg agttagctaa
    16861 attttgtcta cggcaatgtg aaaattgcta cgcgtcatac tatttcttta gttgcgagag
    16921 cagccgacgt tgttgggaaa ggatgacctt ttcgagttct gataaaacac tggatttccc
    16981 gtaggcttct gaaaactcat gacatgtctc cttaaaaatc cctactgatt tctcggccat
    17041 ttcttgtact gtcagatgaa gccactttgt acctacttta atatctttgg ctaaacgggt
    17101 ccaatgggat cccgtaattg ttcctgaatc ggaggaacca ccaatagtca ttgccaactt
    17161 ggttgaaagg ttcggataaa tctttgtcga cactatatcg tagtgaggac tcactctcca
    17221 ctcattcggc tcgaccatca aaaatgaaat attttttgca tgggcatcgc agttcccaac
    17281 aatcacatta aaaataagcc attttaccaa acgctcaaga tcttcaggca agttggaact
    17341 gagttcttcg gcacattgat aacattcttg aaatcccggt ccgccgtctt tttcatattt
    17401 cactttatgg ctgtaggcca aggcctgaca aaaatcttct tgatgaagac gagttatttg
    17461 atttctcact tttttccggt catagcgttc tatcaagtag gcattcacgc ctcccatatc
    17521 cagtaacatt ggcacggcca tttcaagacc catttttttt ccaaacaaac ccattaaata
    17581 ttcactctca ggaaattcca agttttgcgg ccacagcaat acggatatct ccttcgggca
    17641 aaaggttcgc aaaataattc tgcgcctctc tcgagtaagc ttcctcgcgc aaaggcatag
    17701 agtgtgagat cgaatatttt ttaatggatt ttagccaaat cgaagcatat gcaaactcta
    17761 tccgatgctg cgaatcttcc cacaggcttc cgatgagtgt ttcattacaa tagagattta
    17821 acttagagtt catcatgagg atcttttcct gtgcgggctt caagcgtgag tttaacccca
    17881 aacttcctta agacctgaag gactttgccg aggtccaagg attccttctc ccctctttcc
    17941 agctccgaaa aaaacctacg tccgacaccg gcaagcgcgg aggccttctc ttgactcaaa
    18001 cctttttgat ggcgtaattc tcgaactaat ttgctgatat cacttacttt ttttacattt
    18061 acctgcacaa ttcaactccg ttcccgaccg ggagcaaatc ttagttgagt ttaatatagc
    18121 attattttga tcccgaacgg gaacaaaatt gtgacatatc caataaatat gactttgatc
    18181 ccgatcaggc acaacgctgg tattggcatg tacctacacc ccactaattg atagaaaggg
    18241 ctctccgttt tatagaaggg tcttaaattt tctactagac gaagatacgg ctatcataag
    18301 tcgcgaagga gcttattatt cattttgaaa ctcgtagcgc gactttactt ctgtttgagt
    18361 acaaccagaa gtccaatgaa aacattcgga tag
//
LOCUS       DATBTE010000217         2773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1668132, whole genome shotgun sequence.
ACCESSION   DATBTE010000217 DATBTE010000000
VERSION     DATBTE010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668132"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1218)
                     /locus_tag="VIG33_08000"
     CDS             complement(19..1218)
                     /locus_tag="VIG33_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY8270819.1"
                     /translation="MRIREKKKIALVLSGGGIKAAAFHIGVCLALKELGFTTAGGQKE
                     IVRQKYTEDDPLTIRMYVGSSAGAFIASILAAGYPIESLINAFQVGSGHEPTFTEEDI
                     QYLRPLSYRDIFSVNGMNILNLIPDAFLKKSLVAGGIETLLKNGFKLNGLFTTNGLEK
                     YLRKEVFIENDFARLGVELFVIGTQLNHTRKAIFGNFPESSKTGTTKYINYSTISEAV
                     ACSASLPPVFAPYGVTRPDGKKIYYFDGEIRDTLSTHVAADNGADLVISSYSVQPYHY
                     TPEMGSLHKYGIPVIINQALYQVIQQKIAKHIEYQSNMRQIYHEVDSFFKEHNLPAEL
                     RDQLLTKVSEEGKYKPGIDYIYIHPRPQNYEMFFVDHFSLNPDILARIVRIGFKSAYN
                     VLRHHDL"
     gene            1457..1951
                     /locus_tag="VIG33_08005"
     CDS             1457..1951
                     /locus_tag="VIG33_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270820.1"
                     /translation="MTSMPNGAEVPNTTESWLVLMDYSTKYKVSVSTLRRRIKTEDIK
                     FRLEDGKYYIIDEPMSAHPRMHRPSLESDFALVGTHQGNPQAPNCLPIKSDIADRIAM
                     AKKDEPILIAANKLLTELKQAYTQIIQEKEEQTLMLKGEVADLKTLVRILEGENERLK
                     GLIE"
     gene            1960..2268
                     /locus_tag="VIG33_08010"
     CDS             1960..2268
                     /locus_tag="VIG33_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270821.1"
                     /translation="MKTILISITLTLFLGVTAHAETCKRGDIKDCAKVLKAEEGSETF
                     VQTYKNVCSENKTFKCLKRTVRGDVKEEMKYMKEEYSQAYLFSTKDGTDDKIFVLDKK
                     "
     gene            complement(2326..>2773)
                     /locus_tag="VIG33_08015"
     CDS             complement(2326..>2773)
                     /locus_tag="VIG33_08015"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEY8270822.1"
                     /translation="ELNIPADFFEKYDIHVHLPAGAIPKDGPSAGITLTTALVSLMTD
                     TPVRHDIAMTGEVTLQGRVLPVGGIREKCLAALNQGITNVIIPFANAKDLTDIPKQFK
                     EKMNFILAENLDEVFSVAFDKTANGNKKTPGNKKESKKPKAPAAAA"
BASE COUNT          860 a          549 c          604 g          760 t
ORIGIN      
        1 gaactcccgg cttttttatt aaagatcgtg atgtcttaaa acattatacg cggatttaaa
       61 tccaatcctg acaatacgag caagaatgtc aggatttaaa ctaaaatgat ccacaaagaa
      121 catttcgtag ttctgagggc ggggatggat atagatataa tcgattccag gcttatactt
      181 tccttcttcg cttactttcg tgaggagttg atcgcgaagt tcagccggta agttgtgttc
      241 tttaaagaag gaatccactt cgtggtagat ctgacgcata tttgattgat actcgatatg
      301 cttagcgatt ttttgctgaa ttacctggta aagcgcctgg ttgataatca ctggaattcc
      361 atacttgtgg agcgaaccca tttctggagt atagtggtaa ggttgaaccg aataagaaga
      421 gattaccaag tcggcaccat tgtcggccgc cacatgggtg gaaagcgtgt cgcgaatctc
      481 gccatcaaag taatagattt ttttaccatc gggtcgagtg acgccataag gagcaaaaac
      541 cggagggaga gaagcagaac aagccactgc ctcactaata gttgagtagt taatatattt
      601 tgtagtaccg gtttttgaac tttcaggaaa gttcccaaaa atcgccttac gagtatgatt
      661 taattgagtt ccaataacaa aaagttccac acccaatctc gcgaaatcat tttcgataaa
      721 aacttcttta cgaaggtatt tctctaagcc gtttgtagtg aagaggccat tgagtttaaa
      781 accattcttt aagagagttt caatacctcc agcgaccaaa gattttttta gaaaagcatc
      841 aggaatgaga ttgagaatat tcattccatt aacactaaaa atatcgcgat agctcagcgg
      901 tcgaagatat tgaatatctt cttcagtaaa agtcggttca tggcctgaac ccacctgaaa
      961 ggcattaatc aaagattcaa tgggataacc cgcagcgagt attgaggcga taaaagcccc
     1021 ggcactggat cccacataca tacgaatagt gagagggtca tcttccgtgt atttttgacg
     1081 cactatttct ttctggccgc cagcagtagt aaagcccagt tcctttagag ctaggcaaac
     1141 accaatatgg aaggcagcgg ctttaatgcc gcctccactc aaaaccagag ctattttctt
     1201 tttctcgcga attcgcatat caaaattatt tcacgtatta caagattatc aatggaagaa
     1261 tgatccaaaa atcttcatag gagtctgaac acaggcttaa ataggactat gggccagcgc
     1321 ggagaacctg aaaacccagc cttgagtcga gaacttcgcc gagggcccgc ccaatggttt
     1381 gataaatttg acagtccacc tcggtatgat agaactgatt gcgaaagctg gagacggctg
     1441 agtaggcgga gagacgatga ctagtatgcc taatggagct gaggttccaa acacaacaga
     1501 gtcttggtta gtcctaatgg attattcgac taagtacaaa gtaagtgtta gcacactacg
     1561 cagacggatc aaaaccgaag atattaaatt caggctcgaa gatggtaaat actatattat
     1621 cgacgaaccg atgagtgccc atccgcgcat gcatcgcccc tccctagaaa gcgactttgc
     1681 gttggtgggc actcatcaag gaaatccaca ggctccgaat tgtttgccta ttaaatcaga
     1741 catagcagat aggatcgcaa tggcaaaaaa ggacgagcct atccttatcg cggctaacaa
     1801 acttttgact gaactaaaac aagcctacac gcaaattatt caagaaaaag aagagcagac
     1861 actcatgcta aaaggggaag tcgcagactt gaaaacctta gttcgaattc ttgaaggcga
     1921 aaacgaaaga cttaagggcc ttattgaata ggagtctctg tgaaaacaat tttaatatct
     1981 ataactttaa ctttgttcct tggggttaca gctcatgcgg aaacttgtaa aagaggcgat
     2041 ataaaagact gtgctaaagt tttaaaggca gaagaaggtt ctgaaacctt cgttcaaact
     2101 tataaaaacg tttgtagtga gaataagact tttaaatgtc ttaagcgcac ggttcgcggt
     2161 gatgtcaaag aagagatgaa gtatatgaaa gaggagtatt cgcaagccta tctatttagt
     2221 acaaaggatg gtactgacga taaaattttt gttctggata agaaataaaa agagccgcgg
     2281 ttcagacgtg gctcttttta ttttttggag gagcagttcc ttgacttatg ctgccgcagc
     2341 cggagctttc ggttttttac tttctttttt attacctggc gtctttttat ttccattcgc
     2401 tgttttatca aaagcaaccg agaagacttc atcaagattt tcagctaaaa tgaaattcat
     2461 tttctcttta aattgcttag gaatatctgt gaggtctttg gcatttgcaa aagggataat
     2521 cacattagta atgccctgat tcaaagctgc caaacatttc tcgcgaatcc cgccgactgg
     2581 aaggacacga ccttgcagag tcacttcacc ggtcattgca atatcgtgac gaactggggt
     2641 gtcagtcatt aaactcacaa gagcggtcgt cagtgtaatt cctgcagatg gaccatcttt
     2701 agggatagca cctgcaggca agtgaacgtg aatgtcatac ttttcaaaga aatcagctgg
     2761 aatgttgagc tct
//
LOCUS       DATBTE010000218         9515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_849184, whole genome shotgun sequence.
ACCESSION   DATBTE010000218 DATBTE010000000
VERSION     DATBTE010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9515
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849184"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VIG33_08020"
     CDS             <1..409
                     /locus_tag="VIG33_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270823.1"
                     /translation="QNSEKTRNESRKKYFRWIEMWLGPLTKEQTQLLNQHLNQHPFPL
                     QAQIKNKIYILEKFHESRRSPEDLKNFVRNYYNNKYEVQNQEYRQALASYQRELEKFI
                     FQLIQSLNEKQKKLLAENLSEKSTSLEKLSVNN"
     gene            complement(439..1047)
                     /locus_tag="VIG33_08025"
     CDS             complement(439..1047)
                     /locus_tag="VIG33_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4360 domain-containing protein"
                     /protein_id="HEY8270824.1"
                     /translation="MKLLSSVLSVIVVAASLTAEAQSGLTLGQPAYGGTGCPGGSASV
                     TLSPDQTALSILFDSYVAEAGATTGKTLDRKSCNISVPVQVPNGYSVAIFQVDYRGFN
                     LIPAAGAYTRLDTEYFWAGVRGPRFARVYSGPTASNYTQSDGLVAQTLVWTPCGASVN
                     LRVNSSVLAQTNRNQDQTMITVDSADLTSGLIYHLQFRRCTI"
     gene            complement(1263..1862)
                     /gene="pdxH"
                     /locus_tag="VIG33_08030"
     CDS             complement(1263..1862)
                     /gene="pdxH"
                     /locus_tag="VIG33_08030"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEY8270825.1"
                     /translation="MTFDFSLDPFLHFTRLLDEAKSRGEPEYNAMSLATVGMDLKPSV
                     RIVYFKGLVRGGLSFFCNYHSTKGKDIEQNNQVCLNFYYPKEWQQIRINGKAWKLTRE
                     ENVAYFATRHRLSQLGAWASNQSQEIPGTEYFQRRLAEYEKSYENQEVPCPENWGGYH
                     VIPDEFEFWSGKTGRLHERCIYQRQASGPTPWRTFMKAP"
     gene            complement(1862..2599)
                     /locus_tag="VIG33_08035"
     CDS             complement(1862..2599)
                     /locus_tag="VIG33_08035"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY8270826.1"
                     /translation="MKKLIIAGASAGLLIAVGCTKKLDTDQKKASYAIGQQIGGNMKQ
                     QNIEIDTDVMAMAMKDAIKGDSKMTKEEIQQAMMKLQESAMKKQQEAGEENKKKGAEF
                     LEKNKTAEGVKVTASGLQYKVEKEGDGKMPTKTDTVKAHYKGTLIDGTQFDSSYDRGQ
                     PAEFPVQGVIPGWSEALQLMKVGSKYKLFIPPELAYGASGRPGIPANSVLVFDVELMD
                     IVKPGADAAAAAKPEAKAKAEAKPKKK"
     gene            complement(2687..3799)
                     /gene="mnmH"
                     /locus_tag="VIG33_08040"
     CDS             complement(2687..3799)
                     /gene="mnmH"
                     /locus_tag="VIG33_08040"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041583583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEY8270827.1"
                     /translation="MSNIQESELRELFLKERTLIDVRAPVEFAQGSLPGAVNIPIMND
                     DERARVGLAYKSLGREKAVRLGHELVCGELKEARIQAWMAEIKKDSQTVIYCFRGGLR
                     SQITQSWLKDRGVNKPLIVGGYKKARHFLMEEINNFSQNSKFVMLTGPTGSAKTQILN
                     RASTFYPSVDLEALAHHRGSAFGAWEVPQPSQIDFENRLAVSLIKLQDKQVQSRVLLE
                     DESRMIGRCTLPENFFFKMRASPVLYVEEKFDQRVENIFQDYVIGTVIAKGQEGEALG
                     VFSRYRHAVQTISRKLGGLRAQEVLNQLASSEKAYLKERDLEPNKAWIATLLSYYYDP
                     LYAKSLAARSPQVLLRGTSAEILEHLRKFSRGMLTL"
     gene            complement(3792..4853)
                     /gene="selD"
                     /locus_tag="VIG33_08045"
     CDS             complement(3792..4853)
                     /gene="selD"
                     /locus_tag="VIG33_08045"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEY8270828.1"
                     /translation="MSSENIALTQTVQKGGCAAKVAASELRKILSQVTFPPAHPALLV
                     DGGLFDDAAIYKVTEEIALVQTLDFFTPIVDTPKLFGQIAAANSLSDVYAMGGRPKTA
                     MGILAFPIASLPASVIVQVMQGASDVVAKAQANFVGGHSIDDDTLKFGLSVTGFVHPE
                     KIWSNAKAQAGDVLILTKALGTGTMTAALKRQEASEADILEATQSMSQLNNIIDFLSE
                     DSVAAIHAATDITGFGLSGHSMQLAKASHVRLHMDFARVPRFARSLEFLEKGFLTKAH
                     RSNALYTEDAVDQRSLSALQKFLIHDPQTSGGLLLSVKKDKAAQILQQLQGTFAQSSL
                     IGRVLSVEDSSSDFMVIFE"
     gene            4936..5313
                     /locus_tag="VIG33_08050"
     CDS             4936..5313
                     /locus_tag="VIG33_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270829.1"
                     /translation="MKTLIIIFVLSLILPGVSSAVSPDQKSKLNTHLYEKSEGENSVN
                     GKVKAIREVQEETEVFLETKGSGGPFVLPQGLQGRAKMLKSLQKSLKPGGPPVTISID
                     EQQRIKSVEESESSTKAQSEWHL"
     gene            5348..6034
                     /locus_tag="VIG33_08055"
     CDS             5348..6034
                     /locus_tag="VIG33_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8270830.1"
                     /translation="MRILVVEDQVKMANFLKKGLNEVGYAVDIAESGTSAESFMAQSE
                     YDLVVLDVMLPDQNGIDTARHLRRDGYDGPILMLTALSTTKDKIMGLDAGADDYLTKP
                     YSFDELLARVRALLRRKSSATQGSSNSLLQYADLELDLLHRKVRRAGQELSLTTKEFA
                     LLEYLLRNVERPLGRVSIAEHVWDIHFDSESNVIDVYINMLRKKIDAPFNKKLIHTVV
                     GVGYVLKENP"
     gene            6006..7472
                     /locus_tag="VIG33_08060"
     CDS             6006..7472
                     /locus_tag="VIG33_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY8270831.1"
                     /translation="MVTFSKKILKSFRRPSIRMRLTFIFVAIFGTTTILFNTFLFLFM
                     MQTLQQDFDDALFNYAVDISQSIEIGVKGDLNFPPLKLDSGKVLPFPLGTALIQVVHT
                     SGTVLARVGDFGDFNPSFKKDLEHLSKGEEAHYRTISNISKIPSAEADSYRLISFPLD
                     NSTRPQVILQIAVPMTLLETQISNRLTLLQIGIPLVLLFATLGGFFFSSRALAPVNRM
                     IEAAQGINASELDQRVPVPEANDEIKKLSLTLNEMLERIHKAFQSQERFVADASHQLL
                     TPLAILKGELEVLQKQDFNKEEFEGFLKSSLQEIDSLAKIVKDMLLLARVDSGQGGLN
                     LQEIYLDELLIEAIGRIEKIANNKNIRLKFDILEDAEDRKPVRGDYDLLLNLLINIIE
                     NGIKYSPENEMISISLKWKQECNEIVIADHGPGISEGQMPLIFERFSRGANVERDTKG
                     FGLGLAIAQKIAILHQAKLYAQNNSGIGARFHFEIKNI"
     gene            7575..7793
                     /locus_tag="VIG33_08065"
     CDS             7575..7793
                     /locus_tag="VIG33_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270832.1"
                     /translation="MKIVSIVIALAFAGVVAHAEEHAAPAAAAPAATPAATPYPEVKK
                     EVKAKKGHKKGHKKEEATTTTEAAPTTK"
     gene            complement(7878..9185)
                     /locus_tag="VIG33_08070"
     CDS             complement(7878..9185)
                     /locus_tag="VIG33_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HEY8270833.1"
                     /translation="MTPLNPSIMEILGTGIFAVAILHTFVVQKILNWSRRCHKGSILG
                     DFLHLMAEIEIVFGLWAAIFLVLMGLIEGSKAAVEYQAGLDFTEALFVFCIMVIASTR
                     PILGVARASIRFVSVLLRRLFRTSEKITDIFVILTLGPLSGSFVTEPAAMTVTALLLV
                     SLFHSPPERLAYFLMGVLFVNISVGGAMTPYAAPPILMVAGRWGWDFAFVFEHFGLKS
                     ILITVINALILVLYFRKDIQQYCYSLFEAEQKQEKRGMQIPWGVTVLHLVFLVMLVLT
                     AHYEKVFMGVFLFFLGLTTVTRRYQEQLRLRESLLVAFFLGGIIVFGSFQRWWLEPLL
                     RGMSDAVLFKGATLLTAVTDNAALTYLGSQVPHLSEASRYALVAGAIAGGGLTVIANA
                     PNPAGFSILNHKFPGGIVNPLKLFVAAIIPTIVAVICLWLLPH"
     gene            complement(9202..>9515)
                     /locus_tag="VIG33_08075"
     CDS             complement(9202..>9515)
                     /locus_tag="VIG33_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY8270834.1"
                     /translation="YANFMSNDFHLLSVNGHDNMKKGDRTPEGFMPPNLNYACDYLTI
                     WLKIKLIWNLAMTPSEAQAISELVKQNNCSESLLTMSSDDLNSQRESILENSGLCQAS
                     R"
BASE COUNT         2763 a         2256 c         1937 g         2559 t
ORIGIN      
        1 tcaaaatagc gagaagaccc gtaacgaaag ccgtaagaaa tattttcgtt ggattgagat
       61 gtggcttggt cctttgacga aagaacaaac gcaacttttg aaccaacatt tgaaccaaca
      121 tcctttccca ctacaagcgc aaatcaaaaa taaaatttat attcttgaga agtttcatga
      181 gtcccgtagg tcccccgaag atctgaaaaa tttcgtacgc aattactaca acaacaaata
      241 cgaagttcaa aatcaagaat accgtcaagc cttagcgtcc tatcaaaggg aactggaaaa
      301 gtttattttt caacttattc aatctctcaa tgaaaaacag aaaaagcttc tcgcggaaaa
      361 tttatcagaa aagagcactt cacttgaaaa gctctctgtt aacaattaac agagagccct
      421 caagcttaaa ttcaagaact aaatcgtgca tcttctgaat tgcaaatgat aaatcaatcc
      481 tgaagttaaa tccgccgaat cgaccgtgat catagtttga tcctggttac gatttgtctg
      541 agccaaaacc gaactgttca cacgcaggtt cacactggct ccacatggag tccatacaag
      601 tgtctgagcc actaaaccat cggattgagt gtaattgcta gccgttggtc ccgagtaaac
      661 tcttgcgaag cgtggaccgc gaactcccgc ccagaaatac tcagtatcaa gacgagtgta
      721 agcccctgcc gccggaatta aattaaagcc acgataatcc acttggaaaa tggcgacact
      781 gtatccatta ggcacttgca ctggaaccga gatattacaa gatttacgat ctaaagtttt
      841 tcctgtcgta gctcctgctt ctgcaacata ggagtcaaaa agaatactga gtgcagtttg
      901 gtccgggctc aaggtcacac tggcactgcc tcctggacaa ccggtgccac cataggctgg
      961 ttgcccaagt gtaaggccac tttgcgcctc tgcagtcaaa cttgctgcaa caacgattac
     1021 cgaaagaacc gatgataaaa gcttcatata aaaatctccc catggttggt tgaagtggtt
     1081 tcattttttt gaaacagcac ctataaaaag cactcaaaca aagtgaagca aggaatgggc
     1141 catgtttttt ggagacattt cggacctagg aaaaatttga tactaacgtg aaaggacggg
     1201 aggaacttca atgaagttat ctagagaata cgaaattatt cttattcaaa ttcttaatta
     1261 tcttaaggag ctttcataaa agtgcgccac ggagtgggac cagaagcctg acgttgatat
     1321 atacaacgtt catgaagtct tccggtttta ccggaccaga attcaaattc atctggaatc
     1381 acatgatagc cgccccaatt ttcaggacaa gggacttctt ggttttcgta agacttttca
     1441 tattcagcca agcgtctttg aaaatattct gtccctggga tttcttgact ctgattggaa
     1501 gcccaagcgc cgagttggct gagacgatgg cgagttgcaa agtaagccac attttcttcg
     1561 cgggtcagct tccaagcttt accgttgatg cggatctgtt gccactcttt aggataatag
     1621 aaattcaagc aaacttgatt gttttgctca atatctttac ccttggtgga atggtaatta
     1681 cagaaaaaag aaagacctcc gcgaacaagt cccttgaaat agacaattcg aacggagggc
     1741 ttaagatcca tccccacagt cgccagggac attgcattgt actccggctc ccctcgactt
     1801 ttggcttcat cgaggagtct tgtgaaatgc aaaaaaggat ccagactaaa atcaaaggtc
     1861 attatttttt cttaggctta gcttcggctt tagctttagc ttcaggctta gcagcagcag
     1921 cagcatcagc ccctggctta acgatatcca tgagttccac atcgaaaacc aaaactgagt
     1981 ttgcagggat tccaggacga ccagaagctc cataagcaag ttcaggtgga atgaagagtt
     2041 tgtatttaga acccaccttc atcaattgaa gagcttcaga ccaacctggg atcacgcctt
     2101 gaaccgggaa ttcagctggt tgaccacgat cataagaaga atcgaactga gttccgtcaa
     2161 tcaaagttcc tttgtaatga gctttaactg tgtcagtttt tgtaggcatt tttccatcgc
     2221 cttctttttc aactttatat tgcaaaccag aagctgttac ttttacgcct tcagcggttt
     2281 tgtttttctc aagaaattcc gcgccttttt tcttattctc ttccccagct tcttgttgct
     2341 ttttcattgc tgactcttga agtttcatca tagcttgttg aatttcttct ttagtcattt
     2401 tgctatcgcc tttgatagca tctttcattg ccattgccat aacatcagta tcaatttcga
     2461 tgttttgttg tttcatgtta ccgccgattt gttgcccaat cgcgtaactt gcttttttct
     2521 gatcagtatc caacttttta gtacatccta cggcaataag aagtcccgca gaggcacctg
     2581 caataatcaa ctttttcatt tattctcctg ttttctctta attatttaac cttaatatgg
     2641 ttttttaaat ttgaacattt caatctttaa acaaaattct ccattttcaa agagttaaca
     2701 tcccgcgcga aaatttccga aggtgctcca aaatctccgc agatgttccc cgaagaagaa
     2761 cttgaggact tcgcgccgct aaacttttgg cgtaaagggg atcataataa taacttaaaa
     2821 gagtcgcaat ccaggcttta ttaggttcaa ggtcccgttc ctttaaatag gctttctctg
     2881 aggatgctaa ttgattcagc acctcttggg ctcttagtcc ccctaacttt cgcgaaattg
     2941 tctggacggc atggcgatag cgactaaaca cacccaaggc ttcaccctct tggcctttag
     3001 cgatgacagt tccgattaca taatcttgga aaatattttc tactctctga tcaaacttct
     3061 cttccacata aagcactggc gaggcccgca ttttgaaaaa gaaattttca ggcaaggtgc
     3121 aacgtcctat catgcggctt tcatcttcta acaataccct cgactgaacc tgtttgtcct
     3181 gaagctttat taagctgaca gccaagcgat tttcaaaatc aatttgtgaa ggctgaggaa
     3241 cctcccaagc accgaaggcc gatccacgat gatgagctaa agcctccaga tccacacttg
     3301 gataaaaagt cgaggctctg ttaaggatct gagtttttgc actcccggtg ggccccgtca
     3361 acatcacaaa ctttgagttc tgggagaaat tattgatttc ttccattaaa aaatggcggg
     3421 ctttcttata tcctcccaca atcaaaggtt tgttaacccc tcgatccttc aaccaactct
     3481 gggtgatctg cgaacgcaat ccaccacgaa agcaataaat cactgtctgc gaatcttttt
     3541 tgatctcagc catccaagct tgtatacggg cttctttgag ttctccacaa accaactcat
     3601 gacctaatcg aacggccttt tctcgaccca gacttttata agccagtccc acccgggctc
     3661 gttcgtcatc gttcataatg ggaatattga ccgctcccgg cagtgagcct tgggcaaact
     3721 ccacaggggc cctgacgtca atcaaggtcc gctctttaag aaatagctca cggagttcac
     3781 tctcttgaat attactcaaa aatcaccata aaatcggagg aggaatcctc cacagataaa
     3841 actctgccta taagagaact ctgcgcaaag gtcccctgaa gttgctgaag gatttgcgct
     3901 gctttgtcct ttttaacaga aagaagcaag cctccactgg tctgcggatc atgaattaaa
     3961 aatttttgca aagctgacaa gcttcgctga tcgacagcat cttcggtata aagagcattg
     4021 ctccgatgag ctttggttaa aaagcctttc tctaaaaact cgaggcttcg ggcaaacctt
     4081 ggaaccctgg caaagtccat atgcaaacga acatgactgg ctttcgccaa ttgcatgcta
     4141 tgccctgaca aaccaaagcc cgtgatatcc gtagccgcat gaatggccgc gacagaatct
     4201 tcggatagaa aatcgataat attattgagc tgactcatac tctgggtcgc ttcaaggatg
     4261 tccgcttcac tggcctcttg acgcttcaaa gccgctgtca tcgtgccggt ccccaaagct
     4321 ttggtcagaa tcaaaacatc accggcttga gctttcgcat tggaccaaat tttttctggg
     4381 tgcacaaagc ccgtcactga taaaccaaat tttaaagtat cgtcatcaat ggaatgtcct
     4441 ccgacgaaat tagcctgagc tttcgcaacg acatcactcg ccccttgcat cacctgaacg
     4501 atcacggatg cgggaagaga tgcaatggga aaagccaaga tacccatggc ggttttcggc
     4561 cgtcctccca tggcataaac atcgctcaac gaattggcgg cggcaatctg tccaaataac
     4621 ttgggcgtat ccacaatcgg agtgaaaaaa tccaaggttt gcaccagggc tatttcctca
     4681 gtcactttgt agatggctgc atcatcaaat aagcctccgt ccaccagcaa tgccggatgg
     4741 gctgggggga aggtcacctg cgaaagaatt ttgcggagtt cggaggccgc cactttcgct
     4801 gcgcagccgc ctttttggac ggtttgagta agggcgatat tttctgagct cataatgact
     4861 catcttagcg actcacttat tgcactgcaa tttaaatctg gcagaatgga attgatatct
     4921 aaaataggag cgcctttgaa aacactcatt attatttttg tcttatctct gattttacca
     4981 ggagtttctt ctgcggtatc acctgatcag aaaagtaaac tcaatactca tctctatgaa
     5041 aaatcagagg gcgaaaactc cgtcaatgga aaggtgaagg ccatccgcga agtgcaggaa
     5101 gaaaccgaag ttttcctgga aactaaaggg agcggcggcc cttttgttct tccccagggt
     5161 cttcagggtc gcgccaagat gcttaaatcc ctgcaaaaga gtctgaaacc cggcggtcct
     5221 ccggtgacta tcagcatcga cgaacaacaa cgcataaaat ccgttgaaga atctgaaagt
     5281 tccactaaag ctcagtctga atggcatctt taaacaatga ataagtaaaa ataggtggaa
     5341 tttaggcatg cgtattttag ttgttgaaga tcaagtaaaa atggcaaatt ttcttaaaaa
     5401 gggccttaat gaagtaggtt atgcggtaga catcgccgag agcggcacct ctgctgaatc
     5461 cttcatggct caaagtgaat acgatcttgt ggttttagac gttatgcttc cagatcaaaa
     5521 cggcatcgat acagctcgcc atcttcgtcg cgatggctat gacggcccga ttctgatgct
     5581 gaccgccctt tccaccacta aagataaaat catggggttg gatgcgggcg ctgatgatta
     5641 tctcaccaaa ccttattcat tcgatgaact tttagcacgg gtgcgagcac ttttgcgccg
     5701 caaatcttcg gccactcaag gcagtagcaa ttcccttctg caatatgcgg atttggaact
     5761 ggatctctta caccgcaaag tgcgcagagc aggtcaagaa ctcagcctga ccacgaagga
     5821 attcgccctg ctagagtatc ttttacgaaa tgtggagcgc cccttgggac gggtctcaat
     5881 tgctgagcat gtttgggata tacactttga ttccgagagc aacgtgattg atgtttatat
     5941 caatatgtta cgcaaaaaaa tagatgcccc tttcaataaa aaactcatcc atacggtggt
     6001 cggcgttggt tacgttctca aagaaaatcc ttaagtcttt tcgcagaccc agcattcgca
     6061 tgcgcctgac tttcattttt gtcgcgatct tcgggaccac cacaattctt tttaatactt
     6121 ttctatttct cttcatgatg cagaccttgc agcaggattt cgacgatgca ctttttaatt
     6181 atgcggtcga tatttcccaa tccattgaaa tcggcgttaa aggggatttg aattttcctc
     6241 cgctgaagct cgattctgga aaagttttgc ctttccctct gggcacagct ttgattcaag
     6301 tggttcatac ctcaggcacc gttttggccc gggtggggga ttttggggat tttaatcctt
     6361 cttttaaaaa agaccttgag catctttcta aaggagagga agcccactat cgcaccatct
     6421 ccaacatcag taaaattccc tctgctgagg ccgactctta tcgtttgatc agcttccccc
     6481 tggacaactc cacccgtcct caggtgattc tacagattgc cgttcccatg actctcctgg
     6541 aaactcagat tagcaatcga ttgactcttt tgcagatcgg tattccctta gtgctcctct
     6601 ttgccacctt gggcggattt ttcttctctt cccgagctct tgcaccagtg aatcgtatga
     6661 ttgaagcggc ccaaggaatt aatgccagtg aactcgacca acgagttccc gtccctgagg
     6721 ccaatgatga aattaaaaaa ctctccctga ctctgaatga aatgctggaa cgaatccata
     6781 aagccttcca gagtcaggaa cgtttcgtcg cggatgcctc gcatcagtta ctgaccccct
     6841 tagcgatcct gaaaggcgaa ttagaagttc tacaaaagca ggatttcaat aaggaagagt
     6901 ttgaaggctt tctaaaaagc tcccttcaag agattgatag tctggcaaaa atcgtcaaag
     6961 acatgctgtt gttagctcgt gtcgactctg gacaaggcgg ccttaactta caggaaattt
     7021 atcttgatga attgcttatt gaagccattg ggcggatcga aaaaatcgcc aataacaaaa
     7081 atattcgtct caagtttgat atccttgagg atgcagaaga ccgcaaaccc gtgcgtggag
     7141 attacgacct tttgctcaac ctcctgataa acatcattga aaatggcatt aaatattccc
     7201 cggaaaatga aatgatctcc atcagtttga aatggaagca agagtgcaac gagatcgtga
     7261 ttgccgacca tggccctggg atttcagagg ggcagatgcc cctcattttt gaaagattta
     7321 gccggggcgc taatgtagaa cgagacacca aaggattcgg cctaggacta gcgattgctc
     7381 aaaagatcgc cattctccat caggccaaac tatatgcgca gaataactca ggaattggcg
     7441 cacgatttca tttcgagatt aaaaacattt aatctccctt taatgtgagt tttatatacc
     7501 tttgataatc tgattacgtt ttcaggacat gattctgaat tacttaacaa ctaaatagac
     7561 cacatggagg ctctatgaaa atcgtatcta tcgttatcgc tcttgctttt gctggtgttg
     7621 ttgctcacgc tgaagaacac gctgcgcctg ctgctgctgc tcctgctgca actcctgctg
     7681 caactccata tccagaagtt aaaaaagaag ttaaagctaa aaaaggtcac aaaaaaggtc
     7741 acaaaaaaga agaagctact actactactg aagcagctcc tactactaag taatttcgct
     7801 agagctataa ataatagctt taaaaagaga actcttcgga gttctctttt ttttgctttt
     7861 ttaataagta gattttttta atgcgggaga agccataaac aaataacagc tacgatagta
     7921 ggaataatag ctgctacaaa aagcttcaag ggattcacga ttccccccgg aaacttgtgg
     7981 ttcaaaatac taaagcccgc aggattgggg gcattggcaa tcactgttaa tcccccacct
     8041 gcaatggctc cagccaccaa ggcatagcgg gaagcctcgg aaagatgggg cacttgggaa
     8101 cccaaatatg tcaacgcagc attgtctgtc actgccgtca acaaggtcgc ccctttaaaa
     8161 aggaccgcat ctgacatacc tctcaataag ggttctaacc accatctttg aaaagatcca
     8221 aagacaatga ttccgcccaa gaaaaaggcc acaagcagac tctcgcgcaa acgcaactgc
     8281 tcttgataac gcctggtcac ggtcgtcaac cctaagaaaa atagaaaaac tcccatgaaa
     8341 actttttcgt aatgcgcggt caaaaccagc atcaccaaaa agactaaatg aagaacggtc
     8401 actccccagg gaatttgcat tccacgtttt tcctgcttct gctccgcctc gaaaagacta
     8461 tagcaatatt gctgaatatc cttccggaaa tacaaaacta agataagagc gttgatgacc
     8521 gtaataagaa tgctttttaa accgaaatgt tcaaagacaa aagcaaaatc ccacccccac
     8581 cgtcctgcca ccattaaaat aggcggagcc gcataaggag tcattgcgcc tcccacggag
     8641 atattcacaa aaagaacgcc cattaaaaaa taagccaaac gttccggtgg actatgaaac
     8701 aaggaaacca acaagagcgc ggtcaccgtc atagcggcag gttccgtgac gaagcttccg
     8761 ctcaaaggac ccaaagtaag aatcacaaaa atatcggtta ttttttctga ggttctaaaa
     8821 agtcttcgca gcaaaacact cacgaagcga atgctggctc tagcaacccc caaaataggg
     8881 cgagttgaag ctatcaccat aatacaaaaa acaaaaaggg cttctgtgaa atcaagcccg
     8941 gcttgatact caacagcggc tttgctgccc tcgatcaacc ccattaaaac taaaaaaatc
     9001 gcagcccaaa gcccaaagac aatttcgatc tccgccatca aatgcaaaaa atcacccaag
     9061 atagagccct tatgacaccg acgggaccaa ttcaatattt tttgcaccac aaaggtgtgg
     9121 aggatggcga ccgcgaaaat tccggttccg aggatttcca ttatcgaagg atttaaagga
     9181 gtcatgtgag ccctttattt tctaccgcga agcctggcac aagccgctat tttcgaggat
     9241 actttctctt tggctgttca aatcatcaga actcatagtc aaaagtgact cgctgcaatt
     9301 gttctgcttc actaattcgg agatcgcttg agcctctgaa ggagtcattg ccaaattcca
     9361 aatcaatttt attttcaacc aaatggttag atagtcgcag gcgtagttaa ggttcggtgg
     9421 catgaaacct tcaggcgtcc tgtccccttt tttcatattg tcatggccat tcactgaaag
     9481 cagatgaaaa tcattagaca tgaagtttgc gtaga
//
LOCUS       DATBTE010000219         6825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1668811, whole genome shotgun sequence.
ACCESSION   DATBTE010000219 DATBTE010000000
VERSION     DATBTE010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668811"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            422..1126
                     /locus_tag="VIG33_08080"
     CDS             422..1126
                     /locus_tag="VIG33_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270835.1"
                     /translation="MKFRLGMTILSFSFMAIAFQNCSKTHFTSDESEMYFKAETVAPD
                     VPLPSNGGGDDGSQPVETMPPQTMATPAPNPTPADIPQLPPVTDPVAGPVMDPGTTPG
                     MEPSKDHGKCDNSSSHDQAGHKDDKGHKGDAVAGLVECELGSPSLKIKLNQDFDAEHS
                     NSQSSRVCMTENACLKVLNTYAADRKCSMDLGTPYSSGSPAQCTKIFPGSKGTCHNAK
                     VLSDDAIKAILEKMGK"
     gene            complement(1192..3777)
                     /gene="polA"
                     /locus_tag="VIG33_08085"
     CDS             complement(1192..3777)
                     /gene="polA"
                     /locus_tag="VIG33_08085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEY8270836.1"
                     /translation="MKKIYLVDVSSMFFRAYYAIRPLSSPSGVPVNAIYGFLSMITKL
                     FKDEKPDYMVFTYDRKEPSFRKDLYPEYKAHRTVMPEDLAMQIPYIKRLADYLGIPAL
                     EAPQYEADDIIGTLVKLGLKHHHEVVIVSGDKDFGQLIQKHVVLYDTMKDVKYDEAGV
                     LEKWGVRPDQFIDYLALVGDSSDNIPGVAGIGPKGAQKLLEQFKTLEDIYEHLDQVTS
                     KGVREKLERSRENALLSKKLVRIVTDVPVSDNFENYHLRPLQTDNLRALLQELNFKSF
                     EKSLLGEATSHGTLSVITSVPSLPEEIKVSSLSSEMMGKVEAYLHIKEVTVSVEELLP
                     KLHNNESLWGFSDERGLFIGRDQELWAIDGSPEELGAGTDKKNIRWSGFDLKKIFHQI
                     EAHKPLVEWDSSLAAYVVRAGDSTDFDDIYKKFIGTNLPEFASPAQIYKAHLELQHVL
                     RDRLELFDGDKILRQLEFPLVPVLLGMENRGIRIDTALLKIQSAELAEELATLEKEIH
                     HLAGGSFNVASPKQLGVILFEKLGLAMGKKTKTGYSTGEDVLEKLDHPIAAKVLQWRE
                     LAKLKSTYVDALPEMVNTKDGRIHTCFNQALTTTGRLSSTQPNLQNIPIRTKRGQRVR
                     QAFIADKGKKLLSMDYSQIELRILAHISGDPGLQRAFLDDLDIHAATAAEIFNVDLKH
                     VTHDQRRSAKAVNFGIAYGQGAFGLAENLGISNTEAKNIIGRYFDKFGNVREYIESTI
                     KTAHEKGYVETLSGRRRYIEELNSKNQMLKKFGERAAINAPIQGTASDLVKKAMIDIS
                     QSVDLSLNLAMVLQVHDELIFEGSEGELLSHVPELLRIMESTMKLSVPLKVNYAIGRN
                     WDEAH"
     gene            complement(3806..4114)
                     /locus_tag="VIG33_08090"
     CDS             complement(3806..4114)
                     /locus_tag="VIG33_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8270837.1"
                     /translation="MALNSMDVVSSLNKSTIRSLRLRLGWSQADLARRLKCTSAQVKS
                     WEHGTETPTSHYLNEMFLLVKQADTCSHEIHACPLAENMCDRHALGQIEFSQIKEEIE
                     "
     gene            complement(4219..4524)
                     /locus_tag="VIG33_08095"
     CDS             complement(4219..4524)
                     /locus_tag="VIG33_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEY8270838.1"
                     /translation="MSVRKMAISFRGFLNYPGKVTTLCVVLFTITLVMNGLLWRLWGL
                     HRDFERLTVEILGTQNDIEKLNAQLKQAKDPSFIERQARDKLDLVSEKDLVFVFPEQ"
     gene            complement(4572..5384)
                     /locus_tag="VIG33_08100"
     CDS             complement(4572..5384)
                     /locus_tag="VIG33_08100"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HEY8270839.1"
                     /translation="MKQLIWGLIAIFSISIVHAKTILISDIDDTIKNSHVLNKVDAAI
                     NPGYIENLVLGMNVVYGAVKKNEPKSEIFYVTNALKSIMEASHEEFLKLNKFPAGSIR
                     LRESLMQDNFKITELRKILAKEQPDTAILIGDNGEKDILVYEQVKEEHPQIRFLTYVH
                     LAYSHTSREETGGVITSDQIGFVTSLDLMLQLRKEGVVSNAETLSFVDFFTKAFAEDS
                     GMVDTGPTVIPFWSDCSDFIWTAPDEDLVTSANYLAAKKRIFKRCDISRGED"
     gene            complement(5529..>6825)
                     /locus_tag="VIG33_08105"
     CDS             complement(5529..>6825)
                     /locus_tag="VIG33_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY8270840.1"
                     /translation="TPLVQTDVGGGKTLTGNRAFRPPDYSGQDGVLGYGPEAFAIPKG
                     MERSVGFWMDIYSKYTTDQGLIHDTENLDIIYTQIDFKDIVNDESLNRFQKERRKQKL
                     VEDKKKEFVEMLHKLDKVESPEGLNEAEKKVWNLFLNNTEPHKFREAAQKSRIRFQLG
                     QKDRMQSAIFFSGRYIEEMEKIFHEAGLPIELTRLVFVESSFNVLARSKVGASGLWQI
                     MRYTARPYHMMNDAVDLRNHPMSATKLASRLMKDNFNLLRAWPLAMTGYNHGPTGVRK
                     LTEKYKTRELPELIQNVKSSRSFGFASRNFYACFLAVLEVEKNALKYFPKIVWSQPLD
                     AEEIKLPVSVKYKDLLSWFEGNSEKAEVFNPHLSRQTRRGSLAIPAKTLISIPKDRYN
                     QALISLARGEFRYSANMGPEIKSPEEDKIPEIKSAEPEK"
BASE COUNT         1895 a         1603 c         1363 g         1964 t
ORIGIN      
        1 ggccgctacg atggcggcca ggaaggcctt ctcatggaag gaggcactct ttagttatcc
       61 ctcactgctg tgaccaaact ctttctaatt cgcaaaatcc tttaagggat ttcattatca
      121 acaatatgga gggccagttt ctgaaattta tcttattatg ggatgtctaa tagttcttcg
      181 aataggcgcc aatcggccaa tgatgaggcg ttttcttaaa aggaaaaaag ctataaagct
      241 ccttatttta ttacagccac ttttatttct caatctaaga tgaattgaag aaaagtgtga
      301 gagaagctct gttcaattaa tatacaaata acaaagatta gactgttatt cagcataagg
      361 tcttgtttaa ttctttatag gatcggcatc ttctttgctg agtctatatt acgggggtaa
      421 tatgaaattt agattaggca tgacaatcct gtcttttagt tttatggcca tagcgtttca
      481 aaactgttca aagacacatt ttactagtga tgaaagtgaa atgtatttca aagctgaaac
      541 ggtggctcca gatgttccac tgccttcgaa tggtggcggt gatgatggtt cccagcctgt
      601 tgaaacaatg cctccccaga caatggcaac gcctgcaccg aatccaactc ctgctgatat
      661 tcctcagctt ccccctgtaa cagatcctgt agcaggtccc gtcatggatc cgggaacaac
      721 accgggaatg gaaccaagca aagatcatgg aaagtgcgac aattccagca gtcatgatca
      781 agctggacat aaagatgata aaggccataa aggcgatgct gtggctggac tggtggagtg
      841 tgaactaggc agtccaagtc tgaaaattaa gctgaatcaa gattttgatg ccgaacattc
      901 aaattcccaa tcctcaagag tttgtatgac ggaaaatgcc tgcttaaagg tcttgaatac
      961 ttatgcggcc gaccgtaaat gcagcatgga cttaggaacc ccctattcct ctggcagccc
     1021 cgctcaatgc actaaaatat tccctggcag caaaggcact tgccataatg caaaagtttt
     1081 atcagatgat gctatcaagg cgattttaga aaaaatggga aaataattta agaccctccg
     1141 tggaaagact taataaaaaa aacccacatt cattgatgtg ggtttttttt attaatgagc
     1201 ctcatcccaa tttcttccaa tggcataatt gaccttcaaa ggcacactca acttcatcgt
     1261 cgactccatg atccgcaaga gttcaggcac atgagaaagt aattcccctt cgcttccctc
     1321 aaagatcaat tcatcatgaa cctgcagaac cattgccagg tttaagctca agtccacgct
     1381 ctggctaata tcgatcatcg cctttttcac caaatcactg gcagttccct gaatgggcgc
     1441 attgatggca gcccgctctc caaacttttt aagcatctga ttcttggaat taagttcttc
     1501 aatatagcgg cgacgtccag aaagcgtttc cacatagccc ttttcgtgag ctgtttttat
     1561 ggtactttca atatactcgc ggacgttgcc aaatttatcg aaataacggc caatgatatt
     1621 tttagcctcc gtgttggaaa ttcccaaatt ctccgccaga ccgaaggcgc cttggccata
     1681 ggcgataccg aaattcacgg ccttcgcact cctccgctgg tcatgggtga catgctttag
     1741 atccacattg aaaatctccg ccgccgtggc cgcatgaata tccaaatcat ctagaaaagc
     1801 tctctgcaaa cctggatctc cggagatgtg cgcgagaatt cgcaattcaa tttgcgaata
     1861 atccatggac agcaattttt tccctttatc agcgataaag gcttgacgaa ctctctgccc
     1921 ccgcttggtg cgtatcggaa tattttgtaa attcggctgc gtgctcgaaa gacgtcccgt
     1981 ggtggtcaaa gcttgattaa aacaggtatg aatccggcca tctttcgtat tgaccatttc
     2041 cggcaatgca tcaacatagg tcgatttaag tttagccaac tcccgccact gaagaacttt
     2101 cgcggcgatc ggatgatcca acttttccaa aacatcttcg cccgtggagt aacccgtctt
     2161 ggtttttttc cccatggcaa gtccgagttt ttcgaaaagt atcacgccta attgtttagg
     2221 actggccaca ttaaaggatc ctccggccaa atggtggatt tctttctcga gtgtcgccaa
     2281 ttcttcggcg agttcagcgg actgaatttt caaaagtgcg gtgtcgatgc gaatgcctct
     2341 gttttccatt cccaaaagaa ctggaactaa aggaaactcc agttgccgta aaattttatc
     2401 gccatcgaat aactccaaac gatcccgcag cacatgctgt aactcgagat gggctttata
     2461 aatttgcgcg gggctggcga attcgggcag attcgtacca ataaattttt tataaatatc
     2521 atcgaagtcg gtgctgtctc cggcacggac cacataagcg gccaaagaag aatcccattc
     2581 cactaaaggt ttatgagcct cgatttgatg aaatattttt ttaagatcaa agcctgacca
     2641 acgaatattt tttttatccg taccggctcc gagttcctca ggagaaccat caatagccca
     2701 aagttcctga tctcgaccga taaaaagtcc ccgctcatcg ctaaagcccc aaaggctctc
     2761 attgttatga agcttaggta gaagttcctc aaccgaaacg gtcacttctt tgatgtgtaa
     2821 ataagcttcc acttttccca tcatttctga agataaggag gaaactttaa tttcttccgg
     2881 aagactcggc actgatgtga taaccgagag agttccatgc gacgtcgctt cgcctaataa
     2941 ggatttctcg aaggatttaa agtttagctc ctgaagcaga gcgcgcaggt tgtcagtttg
     3001 caaaggacgc aaatgataat tttcaaagtt atcgctcact ggaacatctg tgacaattct
     3061 taccagcttt ttcgacaaca aggcgttttc ccgagagcgt tcaagctttt cacgcacccc
     3121 tttgctggtc acttgatcaa gatgttcgta aatgtcttca agggttttaa actgttctaa
     3181 aagtttttgc gcccctttcg ggccaatccc ggccactccg gggatattgt ccgaagaatc
     3241 tccaaccaaa gccaaatagt cgataaactg atcgggacgc acgccccact tttccaaaac
     3301 gccggcttca tcatatttga catctttcat ggtatcataa agcacaacgt gcttttgaat
     3361 gagctggccg aaatccttgt cgccgctgac gatcaccact tcatgatgat gtttgagccc
     3421 aagttttacc agagttccaa taatatcatc ggcttcatat tgcggagctt cgagtgccgg
     3481 aattcctaag tagtccgcaa gccttttaat ataaggaatt tgcatggcca aatcctcagg
     3541 catgacggtg cgatgagctt tgtattctgg ataaagatct ttgcgaaaag aaggttcttt
     3601 gcgatcataa gtaaaaacca tgtaatcagg cttttcatct ttaaagagct tcgtgatcat
     3661 tgacagaaat ccgtatatgg catttacggg aactcccgaa ggagaactta atggacgaat
     3721 ggcataataa gcacggaaaa acattgagct gacatcaaca agataaatct ttttcataga
     3781 ttcacctgta cttgagtctc aacttctatt caatctcttc tttgatttgc gagaattcaa
     3841 tttgccctag ggcatgccta tcgcacatat tttcagcaag gggacaagca tgtatctcgt
     3901 gactgcaagt atccgcttgt tttacgagta aaaacatttc atttaagtaa tgcgaggtgg
     3961 gagtttccgt cccatgctcc caagatttaa cttgtgctga agtgcacttc aaacgacgag
     4021 cgagatctgc ttggctccac cccaaacgaa gtcgaagaga cctgattgtg cttttattta
     4081 atgaactcac aacgtccata gaatttaaag ccatctttat aactcccatg attttattaa
     4141 taaaatcata aactttagag tttgcatcct attttttgca gcccctttgt agcccctttt
     4201 gtggggcctt gcaataccct actgctcagg aaaaacgaag actaaatcct tttcactgac
     4261 tagatccaat ttgtcacgtg cctgtcgttc aataaagctg ggatctttcg cctgtttaag
     4321 ctgtgcattc aacttctcaa tgtcattttg tgttcctaaa atctccacag tcagtctttc
     4381 aaaatctcta tgaagtcccc aaagtcgcca caaaaggcca ttcattacta acgttatcgt
     4441 gaaaagaacc acacaaagtg tggttacttt tccagggtaa ttgaggaaac cgcgaaatga
     4501 gatggccatt tttcgaactg acatacttta aaggtacggt cttctatttc gagaatcaac
     4561 gcgttcaagg tctagtcctc acctcgactg atatcgcaac gtttaaaaat tctttttttc
     4621 gcggccaagt aattcgctga agttactaag tcttcatctg gggccgtcca aataaaatcg
     4681 ctgcaatcag accaaaacgg aatcaccgtt ggaccggtgt ccaccatacc gctgtcttca
     4741 gcaaaagctt tggtaaagaa atcaacaaag gaaagagtct cagcgttgga aaccacccct
     4801 tcttttcgca gttgaagcat caagtccaga gaggtcacaa aaccaatttg atctgaggtt
     4861 ataactcctc ctgtttcttc ccgactcgta tgggagtacg ctaagtgtac gtaggtcaaa
     4921 aagcgaattt gtggatgttc ttctttcact tgctcataaa cgagaatatc tttctcgcca
     4981 ttatcgccaa tgagaatagc tgtatctggc tgttctttcg ctaaaatttt gcggagttcc
     5041 gttattttaa aattatcttg cataagactt tcgcgaagtc ttatgcttcc cgctggaaat
     5101 ttatttagct tcaaaaactc ttcatggctt gcttccatga tggattttag agcattggtt
     5161 acatagaaaa tttcgctctt aggctcattc tttttcacag cgccataaac cacgttcata
     5221 cccaaaacca agttctcaat ataaccagga ttgatagcag catctacttt atttaagaca
     5281 tgcgagtttt ttatggtgtc atcaatatca gaaattaaaa tcgttttagc atgaactatt
     5341 gaaatactaa aaatcgctat gagtccccat atcaactgct tcatacaaca aaccctttta
     5401 gtctttgtaa gtttttctcg catattcaaa agattaacag cctaggaggc agtaatagcc
     5461 ttttgagtgg ttcccccgta ctggaagcaa caactacgcc aataaaatgg ggtcataaag
     5521 ggacgaattt acttctccgg ctctgccgac ttgatctcag gaattttgtc ttcttctggg
     5581 gacttgatct cggggcccat gtttgccgag tacctaaact ctcccctcgc caaacttatc
     5641 agtgcttgat tgtagcgatc tttaggaatg gaaatcaaag tcttagccgg gatcgccaga
     5701 gaccctctgc gcgtctgacg ggaaagatgg ggattgaaga cctctgcttt ctctgaattt
     5761 ccctcaaacc agctcaaaag atccttgtac tttacggaga ccggcagctt aatttcttcc
     5821 gcgtccaaag gctgtgacca gacgattttg gggaaatact taagcgcgtt cttttcaact
     5881 tcaagcaccg ctaagaaaca cgcataaaaa tttctagagg caaagccgaa gctccgactg
     5941 gattttacat tttgaattaa ttccggcaat tcgcgcgttt tgtatttttc agtcagcttt
     6001 cttactccag tcggtccatg attataaccg gtcattgcca atggccaagc ccgcagcaag
     6061 ttgaaattat ctttcatcaa tcgcgatgcc aatttagtgg cggacatagg gtgattgcgc
     6121 aaatccacgg cgtcattcat catatgataa gggcgagcgg tgtagcgcat gatttgccac
     6181 aagccactag cccccacttt cgagcgagcc aagacattga aagaactttc cacaaatacc
     6241 aatcgggtca actcaattgg caggccggcc tcgtgaaata ttttttccat ttcttcgata
     6301 taccggcccg agaaaaaaat cgcggattgc atacggtctt tttgacccaa ctgaaaacga
     6361 atgcgcgact tctgagccgc ctctctgaac ttgtgaggct cagtgttgtt cagaaacagg
     6421 ttccacactt tcttttccgc ctcgtttagg ccctcggggg attccacctt atccaactta
     6481 tgcagcatct ccacaaattc ttttttctta tcctcaacta gcttctgctt acgtctttct
     6541 ttttggaaac gattcaagga ttcatcattt acgatatctt taaaatcgat ttgcgtataa
     6601 atgatatcaa gattttccgt atcatgaatc aacccctgat ccgtggtgta tttggaataa
     6661 atatccatcc aaaaacctac agagcgctcc atgcctttgg gaatagcgaa agcctcagga
     6721 ccgtaaccca agacgccgtc ctgcccggaa taatccggag gtctaaaggc tcgattccca
     6781 gtcaacgttt ttcctccacc cacatccgtt tgtaccaggg gagtg
//
LOCUS       DATBTE010000220         3474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2284201, whole genome shotgun sequence.
ACCESSION   DATBTE010000220 DATBTE010000000
VERSION     DATBTE010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2284201"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..801)
                     /locus_tag="VIG33_08110"
     CDS             complement(166..801)
                     /locus_tag="VIG33_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270841.1"
                     /translation="MDINDLVKIPDENRDPQWETDFFVQLSKSNLKLLSPDPQTGPDG
                     WPYLLTETSSEAEEPAQKILQWLATKGMGLVVNPQKEYPDFVFSYGMVWHFRETGLFY
                     RAKPEVSTGSFELSEGAQLHAGEPTPQYLPHYVRSILKQFFLDQSVFAPKILVMSSDR
                     VNYDLAFSLESLGNPPTKEHQGIAEAISWFLPPHYSIALVSEKGFPAFVNL"
     gene            complement(935..1888)
                     /locus_tag="VIG33_08115"
     CDS             complement(935..1888)
                     /locus_tag="VIG33_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8270842.1"
                     /translation="MAGKNKLNELYIERIYDAPVKMVWDAWVDPKQVAQWWGPRGFTL
                     TNHSKDVRTGGHWNYTMHGPDGVDYPNKTKYLEVEKYSRMVYDHGGNDDQPPMFRVTV
                     NFIDLKGKTRMEMTMALATAEAAAETKKFIKKVGGNSTWDRLAEYLEMESTKQDIFVI
                     NQTFDAPLNLMFEMWTDPKHLGHWVGAVGSKINYLKADIKPGGSAFYCMTGVGDTKIY
                     GNANYLEIVKPHRFVYTQSFCDENEKIARHPMVPTWPKVMKTTVSFEAEGPDKTRVNL
                     KWEVVGDATPTEHETFNKAKAGMSQGWGGSFDKLEEYLAKQ"
     gene            complement(1872..2237)
                     /locus_tag="VIG33_08120"
     CDS             complement(1872..2237)
                     /locus_tag="VIG33_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8270843.1"
                     /translation="MKQAEDKLTLTFMALSDPTRRAILSKLSSGEATVQELSEPFDIS
                     APAITKHLKVLEKAGLISRGRTAQWRPCRIETEPLKEAADWIGYYRKFWEESFDRLDV
                     YLKTITTKKRKGQKNGRKK"
     gene            2748..3128
                     /locus_tag="VIG33_08125"
     CDS             2748..3128
                     /locus_tag="VIG33_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270844.1"
                     /translation="MDDILGIALTQLDEELTRRSLNMEIVICGAYALHLLGYSRSEHT
                     LDVDSITTLSSVEIQQIIEAIGQKLGLGPHWLNDLVLLKPTSEEIGEAIIFIKESNSP
                     PPNASKKIIADFEETLYDLKKTAK"
     gene            3103..>3474
                     /locus_tag="VIG33_08130"
     CDS             3103..>3474
                     /locus_tag="VIG33_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270845.1"
                     /translation="MISKKLQNKTTNDEFKQMAHYFGIAIQVELSDITQKNPNLIYMD
                     IELFFLSATLHLKTSRIAEGFLCWLLKFGHLLSPSKIRRLILSGQGFDPAVLGGFIEF
                     LIENNIMPNQWKIIKPFCKKKR"
BASE COUNT          993 a          737 c          712 g         1032 t
ORIGIN      
        1 catcttgaag atatgaatct agagatatcg ggctgtattg caagaaccca gctcttaggc
       61 aaaaaaggtt tccatattgc aattgattac agcgaagacg cccctgaata ttggcgcgaa
      121 tgcttaatgg atttgctccc cgttcccgga gagcttgaat agaccttaaa gatttacaaa
      181 ggctgggaaa cccttttcac tgaccaatgc tatggaatag tgaggaggta gaaaccagct
      241 gatggcttca gcgattcctt gatgttcttt cgtcggggga ttccccaaag attctaaaga
      301 aaacgccaaa tcataattta ctctatcact gctcatgacc aaaatctttg gtgcaaacac
      361 gctttgatct aagaaaaatt gctttaaaat actacgcaca taatgaggta aatactgagg
      421 cgtcggttca ccagcgtgaa gctgagcgcc ttcactcaac tcaaacgagc cggttgaaac
      481 ctcgggctta gctcgataga aaagtcccgt ctctctgaag tgccaaacca ttccgtaaga
      541 gaaaacaaag tcagggtatt ctttttgggg gtttacgacc aaccccatgc ctttagttgc
      601 aagccattgc agaatctttt gcgcaggctc ttcagcttct gaagaagtct ctgtcagcaa
      661 ataaggccat ccgtcagggc ccgtttgagg atctggtgaa agcaacttca aattgctttt
      721 agacagctgt acaaaaaaat ccgtttccca ttgaggatcc cggttttcat caggaatttt
      781 aactaaatca tttatatcca taaggagtga gtactagctt aaaggtttta agaaaacaag
      841 taactctatg taataaataa aatccaccgt aaaacgagtg caggctcaga gcccaagaca
      901 ggtccgacat cacgtatctg ccttggaaat ttaactattg tttggctagg tactcttcga
      961 gtttatcaaa gcttccaccc cagccttgtg acattccagc tttggcttta ttgaaagttt
     1021 catgttcagt aggtgtagca tcgccaacca cttcccattt gagattcact cgggttttat
     1081 caggaccctc agcttcgaat gaaacagtcg tcttcatgac tttaggccaa gtgggaacca
     1141 tcggatgacg agcaattttt tcattttcgt cgcagaagga ttgggtatag acaaagcgat
     1201 ggggtttaac gatttccaga tagttggcat tgccatatat ctttgtatca ccgacgcccg
     1261 tcatgcagta aaaggcgctt ccacccggtt taatgtcagc ctttaaataa tttattttag
     1321 aacctactgc tcccacccaa tgaccaaggt gtttaggatc agtccacatt tcaaacatca
     1381 agtttagtgg agcatcaaag gtttgattga taacaaatat atcttgtttt gtggattcca
     1441 tttctaaata ttcggcaaga cgatcccaag ttgagttccc gccaactttt ttgataaact
     1501 tcttggtttc ggcagcagct tcagccgtag ctaaggccat agtcatttcc attctagttt
     1561 tgcctttaag atcaataaaa ttgacagtca cacgaaacat cggtggttgg tcatcgtttc
     1621 caccatggtc ataaaccata cgggaatact tttcgacttc gagatatttg gtcttgttag
     1681 gatagtcgac accatcgggt ccatgcattg tgtagttcca atggccgcct gttcgcacat
     1741 ctttgctatg attggtaagt gtaaacccgc gcggtcccca ccattgagcc acttgtttag
     1801 gatccaccca agcatcccag accatcttca ctggagcatc ataaatgcgt tcaatataga
     1861 gttcatttag tttatttttt ccggccattt ttctgtccct tcctcttctt cgtagttatt
     1921 gtttttaaat aaacatcaag tcggtcgaaa ctttcttccc aaaattttct atagtagcca
     1981 atccaatcgg cggcttcttt aaggggttca gtctcaatac ggcaaggacg ccattgtgcc
     2041 gttctccctc tagaaataag ccccgccttt tcgagtactt tcaaatgttt ggtgatagcc
     2101 ggcgctgaaa tatcaaaagg ctcggagagc tcttgcacgg tggcttcgcc gctggacaac
     2161 ttagataata ttgctctacg ggtagggtct gagagagcca taaatgtcag agttaattta
     2221 tcttctgctt gcttcataat tacctaatcg gttaattaac taattagtta attataaagg
     2281 aaaaaggacc tttgtcaata gggtcaaaat tggaagaaga ggtctcaatt ccagttaaat
     2341 gggtttaacg gcctggttgc atgaatcgag attcatagca gtgggtttac acccggcgag
     2401 accctttggg tgaatcgcgt aatacgctcg caagttgcat attacgcgat cgggtttaag
     2461 ggtgcttgat taataacact gacaggcatt ccagaaattt tgcagtgtgg tggatggaag
     2521 ggaggttgaa tcctcgaatg ttgccagggg agggaagtgg catcgttgct attgtgataa
     2581 cggagcagcc tttcaaggac gatccgctct aggaactttc cgccaaaatt tctctatgcc
     2641 tttgatatta tacaataaaa tcagatctta ctttttaata ttaagtacct atttttattg
     2701 agtattttat agtattatta tagtacgaac ccttggagag ctataaaatg gatgatattc
     2761 taggtattgc cctcactcaa cttgacgaag agctaacaag gcgatctctg aatatggaga
     2821 tagttatctg tggtgcctat gctcttcatt tgctcggata ctcacgatct gaacacactc
     2881 tggatgttga tagtatcacc acgctttcct cggtagagat tcaacagatt atcgaagcaa
     2941 taggtcaaaa attaggtctt ggacctcatt ggctcaatga tcttgttcta ctgaagccca
     3001 catctgaaga aattggcgaa gctattattt tcataaagga gagcaactct ccgccgccga
     3061 acgcttcaaa gaaaattatc gctgattttg aggagactct ttatgatctc aaaaaaactg
     3121 caaaataaaa ccaccaacga cgaatttaaa cagatggccc attattttgg aattgccatt
     3181 caagttgaac tttcagatat aacgcagaaa aatccaaacc tgatctacat ggatattgag
     3241 ctctttttct tatccgcaac acttcatctt aaaacctcga gaatagcgga aggatttctt
     3301 tgctggcttt taaaatttgg tcacctacta agcccttcaa aaataagacg acttatttta
     3361 tcaggacagg gtttcgatcc tgctgtcctg ggcggattca tagagttttt gattgagaat
     3421 aatattatgc ccaatcaatg gaagataatt aaaccatttt gcaaaaaaaa acgt
//
LOCUS       DATBTE010000221         1784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1669104, whole genome shotgun sequence.
ACCESSION   DATBTE010000221 DATBTE010000000
VERSION     DATBTE010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669104"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VIG33_08135"
     CDS             complement(<1..378)
                     /locus_tag="VIG33_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270846.1"
                     /translation="MSRRSFLSLFSVIALAMGLVASLVSCTKARQPQKIPSYQPFSIV
                     QNIPLTSTCDEKCVSLKKEFRYVVYVGQQIYCYWDEKKKETGSDFDQIAADIESRISS
                     TTSAQDYYLLLMEWAASFHDGHVN"
     gene            complement(489..>1784)
                     /locus_tag="VIG33_08140"
     CDS             complement(489..>1784)
                     /locus_tag="VIG33_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270847.1"
                     /translation="FSLLLMVFGINDSAKAGSVYQTLCSKAGLNKNQVRAYENNQGEY
                     VLNIQMFETYKHFRACTHKDPRVKINPSFEPQLEFLENELKKHQDVQSQEWTDLNHRS
                     FEIFRETQTLYQEYIENLISGKTSAAQKNVLEKIKKRITTVRINKSHDCNSFNSNYNF
                     VSHSIDTCNELLSYPLTTLMAIYAHEITHSIDPCSLQFPLSHIAKENPFSSNQLIGEN
                     AYNDAYTALPLEWALEDLFLEKDQLRTVIAPLRFELNPFQKALECLQSEKSIHAQKRK
                     KPLEKSNVKDEEHMCSSSLQDGQMAETFADWMAYEVLAYSFKKQKSPELSEQIFEGTL
                     NVVANECEQLESEIEVEMRKAITASAKCRSDNENFFNFQKRLRDQTLLDTHPHGELRI
                     NKLYGAQPTLRRLLQKHLRTEKDSLHDKVIYCTSVKTDR"
BASE COUNT          498 a          343 c          390 g          553 t
ORIGIN      
        1 attgacgtgg ccatcatgaa aagaagctgc ccactccatt aatagcaagt aataatcttg
       61 cgcggaagtt gtagaggaga ttcgagattc gatatctgcg gcaatctgat caaaatcaga
      121 gccggtctct tttttctttt catcccaata acagtaaatc tgctggccga catagacgac
      181 gtagcgaaat tcttttttta aagaaacaca cttctcatca caggtcgaag ttaaaggaat
      241 attctgaacg atggaaaacg gttgatagga ggggattttc tgtggctgcc ttgcttttgt
      301 gcatgatact aaactcgcaa caagccccat agccaatgcg atcaccgaga ataggctcaa
      361 aaaagatctt cgactcattt cacacttccc tcgaatcgac ttcatctaac ataggccgtg
      421 atcgagatca atggatgagt agaaagccca gcacaaattt gggggtaatt gatccttagc
      481 atatgtaatt aacgatcagt cttgacggac gtgcagtaaa taaccttatc gtggagagag
      541 tctttttcag ttcgaagatg cttttgcagt aaacgacgta aagtgggctg ggcaccgtaa
      601 agcttattaa tgcgaagctc gccgtgagga tgagtgtcca agagagtttg atcacgcaac
      661 cttttttgaa aattgaagaa attttcgtta tcagagcggc actttgcgct tgcggttatg
      721 gctttgcgca tttctacttc aatttcgctt tcgagctgtt cacactcatt tgcgaccaca
      781 tttaaagttc cttcaaaaat ttgttcgcta agttctggac tcttttgttt tttaaaagaa
      841 taggcaagaa cttcatacgc catccaatcg gcgaaagttt ccgccatttg gccatcctgt
      901 aaagatgaag aacacatgtg ttcttcatct ttgacattgc ttttctcaag tggttttttg
      961 cgtttttgtg cgtgaatgga cttctcggac tgaagacact ctaaagcctt ttgaaagggg
     1021 ttcaattcaa atcggagtgg cgctatcact gttcgaagtt ggtccttttc caaaaacaga
     1081 tcctctaggg cccattcaag cggaagagcg gtgtaagcat cgttgtacgc gttttcgcca
     1141 atcaattgat tgctagagaa gggattttcc tttgctatat gagaaagggg aaattgcaat
     1201 gaacaagggt caatgctgtg agtgatctca tgggcataga tcgccatcaa agtggtgaga
     1261 gggtaagata agagttcatt gcaagtgtcg attgaatgag atacaaaatt ataatttgaa
     1321 ttaaaggaat tgcaatcatg ggacttgttt atacgaaccg tagtgatcct tttttttatt
     1381 ttctctaaaa catttttctg tgcggcactg gtttttccgc tgataagatt ttcaatatat
     1441 tcttgatata gtgtctgcgt ttcacggaag atttcgaaac ttctgtggtt gagatctgtc
     1501 cattcttgag attgaacgtc ttggtgtttc ttcaattcat tttcaagaaa ctccaactga
     1561 ggctcaaagg aagggtttat ttttaccctg ggatccttat gtgtgcacgc acgaaaatgc
     1621 ttgtaagttt caaacatttg aatattcaac acatattcgc cctgattatt ttcgtaggcc
     1681 cgaacttgat ttttgttaag accggctttt gaacacaggg tttggtaaac agaaccggcc
     1741 tttgctgagt catttatgcc aaagaccatg agcaagagac taaa
//
LOCUS       DATBTE010000222         2672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1465133, whole genome shotgun sequence.
ACCESSION   DATBTE010000222 DATBTE010000000
VERSION     DATBTE010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465133"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VIG33_08145"
     CDS             complement(<1..266)
                     /locus_tag="VIG33_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEY8270848.1"
                     /translation="MAFLPIPLVKMSGAGNTFVLVDATKTSTWLDIEANLNIKRRSFV
                     KMICDRTLGVSADGVLFLQEGEVGCDYTWDFYNADGSSAEMCGNA"
     gene            complement(423..1298)
                     /locus_tag="VIG33_08150"
     CDS             complement(423..1298)
                     /locus_tag="VIG33_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270849.1"
                     /translation="MILFFLKRAVLTGTALFFFSRAQAVSIESRSKKADTSKDIIEKA
                     YNLSLQKDRSQAINILVNAIKQENVRSENISELKKTLQKVSYLFYSDRAQQAYELALS
                     LRRTDPPQALQKMNEALRIETDNLTLFNEMQRMLLIKGDCSAVLDNVLKERLQDPFDE
                     SLVLLQAQAQVCLGKWEDLKKTHDSFDLKASNQTKFWLSLEIDLALHEKNLTRAKELV
                     THLQKLDPKYPELHYWQWRVATGSEKSALGHKYINGCKNISAALYRQYMTDANLCRKV
                     GEVETATKNFNGTNE"
     gene            complement(1298..1870)
                     /locus_tag="VIG33_08155"
     CDS             complement(1298..1870)
                     /locus_tag="VIG33_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270850.1"
                     /translation="MFKAIIATLGLAFTVQANAAPTVFEAINHAQQVSIMDQAKAQGL
                     DWKVGDQNDYTLDMGFLKGSMTMRLREIAAEGYWLDQDMDLGFAGKQQAQMLLDPNTG
                     EVKKMIVGGKEQEIPKSNIEVVEVTEDHITVPAGTFDCIHARIKDKDTNKETNAWVNP
                     QLIPLSGMLKTIQPSQFGDVTIELKSFQKN"
     gene            complement(1945..>2672)
                     /locus_tag="VIG33_08160"
     CDS             complement(1945..>2672)
                     /locus_tag="VIG33_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEY8270851.1"
                     /translation="NQGIAKLEQYYYTPQPYIVIVVEEFADLMAVDKVNVEQTIIRLA
                     QMARACGIHLMLAMQSPRKEVVTGLIKTNIPGRISFKVAGKMDSRIILDEMGAERLLA
                     NGDMLFLAPGVSKPMRHHGPFLKDSEINGVAKFWSDQAEPEFDPLAMKALDGFAGGGS
                     SSGDFAGASGEGLDEPEFDEKYDEILSWAASQKEVSASLIQRKFRLGYPRAARIIEIF
                     EKEGVVGPAHGSKPRQVLVNSLR"
BASE COUNT          723 a          613 c          503 g          833 t
ORIGIN      
        1 gcatttccgc acatttccgc actcgagcca tctgcattat aaaagtccca cgtgtaatca
       61 caacctactt caccctcctg caaaaataaa acaccatccg cagaaactcc caatgtccga
      121 tcgcagatca ttttaacaaa ggagcggcgt tttatattga ggtttgcttc aatatccagc
      181 caggtggatg tcttggtggc atccactaag acgaaggtat ttcctgcacc agacatttta
      241 accaggggaa tcggcaaaaa agccattcac atcacctttt ctgattattc tttttttcat
      301 cttttttgtt gagatagatt ggctctccaa tcccaataga cgggggctca agtggaggca
      361 aaggccttcc cttgacccca caggcgatca taccgaaaac cataaaaatc gagaaaatcc
      421 ctttactcat tagtcccatt gaagttcttg gttgctgtct caacttcgcc caccttacga
      481 cacagatttg catctgtcat atactgtctg taaagagccg ctgaaatatt cttgcaccca
      541 ttaatatatt tatggccaag tgcagacttt tcactgccag ttgcaacgcg ccactgccag
      601 taatgcaatt ctggatattt aggatctaac ttttgaagat gagttactaa ctctttagct
      661 ctcgtcaggt ttttttcatg gagagctaga tctatttcaa gactcagcca aaatttggtc
      721 tgatttgaag cttttaggtc gaagctatca tgagtctttt ttaaatcttc ccattttccg
      781 agacaaacct gtgcctgagc ttgaagtaaa actaagcttt catcgaatgg atcttgtaat
      841 cgctctttga ggacattgtc taaaaccgcc gaacagtctc ctttaattag gagcatcctc
      901 tgcatctcgt taaatagagt tagattatcg gtttctattc tcaaagcctc attcattttc
      961 tgcaatgctt gagggggatc cgttcgtctt aacgaaagcg ccaattcata ggcttgttga
     1021 gctcgatcgg aataaaacaa atatgaaact ttttggagcg tttttttaag ctcggaaata
     1081 ttttcgctac gaacattttc ttgcttgata gcatttacca gaatgttaat tgcttgggag
     1141 cgatcttttt ggaggctgag attataagct ttttcaataa tatctttaga agtatcggct
     1201 tttttcgatc tgctctcgat cgaaacagct tgggccctgg aaaaaaagaa aagagctgtc
     1261 cccgtaagga cagctctttt taaaaaaaat aaaatcatta gtttttctgg aaggatttta
     1321 actcgatagt tacatcaccg aattggcttg gttggatagt tttcaacatt ccagaaagtg
     1381 gaataagttg tgggttaacc caagcatttg tttctttgtt tgtatctttg tctttaatgc
     1441 gtgcatggat gcaatcaaaa gttccggcag gaacagtgat atgatcttct gtcacctcaa
     1501 ccacttcgat atttgatttt ggaatttctt gctctttacc gcctacgatc atttttttaa
     1561 cttcgccggt atttggatcc aaaagcattt gagcttgttg ctttccagca aagcccagat
     1621 ccatatcttg atctaaccaa tatccctcag ccgcgatttc acgaagtctc atagtcatag
     1681 atcctttaag gaatcccata tccaatgtgt agtcgttctg gtctccaact ttccaatcta
     1741 aaccttgagc tttagcttgg tccataattg aaacttgttg ggcgtggttg atggcttcaa
     1801 acactgttgg tgctgcatta gcttgaactg taaatgccaa gcctagagtt gcaatgatag
     1861 ctttaaacat gatgtcctcc tcgaacggtt gttatggact acgatcaaat catgttcatt
     1921 aaaggagtca agtttaggag tttactatct taaactattc acaagaactt gacgcggctt
     1981 gctgccgtgg gccggcccta caacgccctc tttctcgaaa atttcaatga ttcttgccgc
     2041 tcgtggatag ccaagtcgaa attttctttg aataagcgac gcacttactt ccttttgact
     2101 agcagcccaa gaaagaatct catcgtactt ttcatcaaat tctggttcat ctaatccctc
     2161 tccactcgct cccgcgaaat cacctgagct agagcctccg ccggcaaagc catcgagtgc
     2221 tttcatagct agagggtcga actcaggctc tgcctgatct gaccagaact ttgcaactcc
     2281 attaatttct gaatctttga gaaaaggacc gtgatgacgc attggttttg aaaccccagg
     2341 agctaagaat aacatatcgc cattggccaa gagacgctct gctcccatct catccaatat
     2401 aattcgcgaa tccatttttc cagccacttt aaaactaata cgcccgggaa tattggtttt
     2461 aattaaacca gtcacaactt ctttacgcgg cgactgcatg gcgagcatta agtgaatacc
     2521 acaagcccgt gccatttgcg ctaaacgaat tatagtttgc tccacattca ctttgtcaac
     2581 cgccataaga tcggcaaact cttccactac aattacaata taaggttgag gcgtgtaata
     2641 gtactgctca agttttgcga tgccttgatt tt
//
LOCUS       DATBTE010000223        13464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1259432, whole genome shotgun sequence.
ACCESSION   DATBTE010000223 DATBTE010000000
VERSION     DATBTE010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 13464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13464
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259432"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VIG33_08165"
     CDS             <1..292
                     /locus_tag="VIG33_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270852.1"
                     /translation="KKLNLKEWERAEPKAAAKVKRRNASLRNKKIKTGNIKVPDSAFK
                     DENITAHISIKLPLTLFKELRRLSLNEKYDGKYKVLIINVLTKYIKKASKRK"
     gene            536..1033
                     /locus_tag="VIG33_08170"
     CDS             536..1033
                     /locus_tag="VIG33_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270853.1"
                     /translation="MQTPPTSPEYKVSSVEGLPFLQGGMKAICFLEHKENKFVNAKTM
                     FSKSKDDVQKAIRYGIQQWLQGVPAPKRHHGWNQSQHGGAYTNCYVFKHVAKGVRFYG
                     CLHHPVANGRALICCVIHYAIKREDEVDTYILDDINQFLAKDEFKAALEGFWNELQSK
                     QGDVQ"
     gene            1030..1596
                     /locus_tag="VIG33_08175"
     CDS             1030..1596
                     /locus_tag="VIG33_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8270854.1"
                     /translation="MKKSKGKETYEKNWTESDIGNFLYYVGADFVDQLQASFDTLGIT
                     QRSFAQKLNVSEGRISQVFNDPGNLTLDTMIKWGRAAGLKTGVILYNDGDVKNVRGPL
                     SGSIFIECWKRLGSPIEMPNTQSNFQGSFRSQYDNPRQNTPDDKAVCEMQTALVRTCS
                     GGQATLVKRFLSKQYCNEPQSEEFYTVQ"
     gene            1617..2120
                     /locus_tag="VIG33_08180"
     CDS             1617..2120
                     /locus_tag="VIG33_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270855.1"
                     /translation="MMMIVSVPPKELSDAGVIAKSVNLGSIRLRSAHVDDANYELFLD
                     PNDTSEITLNIDYKSEMKELIEGKSLTSFVKVEAKLLKKEAFIRYMVQYQVDYTLPSV
                     PFPENLSREMFNAFARHNGLLNSWPYIRSQISNLSNDAGFPVMLPLLKITAQKDEAIN
                     SEVVKTN"
     gene            complement(2230..2322)
                     /locus_tag="VIG33_08185"
     tRNA            complement(2230..2322)
                     /locus_tag="VIG33_08185"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2286..2288),aa:Ser,seq:gct)
     gene            2490..2566
                     /locus_tag="VIG33_08190"
     tRNA            2490..2566
                     /locus_tag="VIG33_08190"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2524..2526,aa:Arg,seq:acg)
     gene            2765..3757
                     /locus_tag="VIG33_08195"
     CDS             2765..3757
                     /locus_tag="VIG33_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEY8270856.1"
                     /translation="MKNVFFGVIAFCFVFALEAQALADQMYGVLMVVKGSVKIQNTSK
                     QSSDAKVGGKVLEGETIITGADARAKVVMSDRNVINVTPNTQVTITSYKNDSATGEKN
                     VELNLLKGKVRNNVEQTYDGEKSKFLIKTPTAVAGVRGTQFLAGFDPGTRMTSIVTFK
                     GSVSLASLGANGKIIGVPVTVKKGEMTTAAPNAPPEAPKAVPKEDLKKMDGDSTASLQ
                     NKESKEVKESKDSKEPKEAKDSKDSKEASNGKEQKRDVASDAPAAKEPSAAQPTMIDR
                     SDIDPNQATTVNIPTTTSQPTPPPKSTPPPPISTPSPLVRPGKSNVNVRPVIQN"
     gene            3773..5797
                     /locus_tag="VIG33_08200"
     CDS             3773..5797
                     /locus_tag="VIG33_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8270857.1"
                     /translation="MKRTAFILMALCLGAISYAKETKISQSQAILVELRDPAGEQEER
                     LIRVKEVESNKVTQLTLQKEASSPQVWNGSFIIQFFVGDNSAKTLDFYDPTNSPYYAY
                     ISDGGGKSVQKIILFKTPEELSLFEVTMAEEKQKAAEKQEKQIAKQIQVVAPKGAKAP
                     ISAEKLNQLVRQQGRLQETTQMNIEEAQAKKRMALLEQQQKMSEEAKQKKKKEAAESA
                     KKADAFYASQKYSQAEKLYATASELDPEAEAYFYRYGVSLYKVGNYNKSLAVLSMADV
                     SGDNSVEKDYYVALNHMKLKDYDKARKEFVEIREENSPELSPMASFYAGNIEYQQQKF
                     PEARKSMEYVIDNSKEPSLDKSAEDMLEQIDRMESFYESKKEKYRFKIYSGLIYDTNI
                     LNVAENNVSTDVKAWRLSYGASVLGMIHRTMTSDFGAELAVADYYSMNSTLNNDGTVQ
                     AADALEMDLNLPYHQEIKLNKNQMSLEVIPSYKNIYMATAGGSRALAIKTTALSTTLS
                     APIKSDLFLATKLDLSSDQSLVATSVGDDDLSGPKYGLTFTPSKLLDLKGEKILSGEL
                     SYLKTQSNGINNRSAKTGLAISYGFPGVSKGTSSLRLDYTSQAYAEATTPRTDTNMIL
                     TGSYTKDFNKRWNMLLSLQYTSALSGVESYNYNKFLVTSLFTYTTSILQK"
     gene            complement(5839..9510)
                     /locus_tag="VIG33_08205"
     CDS             complement(5839..9510)
                     /locus_tag="VIG33_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270858.1"
                     /translation="MRISNRFNKLLLAAMMVSVGFISCTRAAKETRSTISFSLPQNAQ
                     SFDTLIHVSVNITGSGIPAPIVFNWDLDSKGSATAATSPSSFALDIPQGPDRLIQVLA
                     VYGDSSSSNSGGGAMKFYYGDIIKTLAAASEDVPVVVTSVGAGATIISGRIQGRYLSS
                     ASNSTNVSNATDGPTSRIDIVYVPPGGKTPMIVERAMMISGWFQFFGLIGAKLGYRME
                     DGKFLWDGPVDLSETSFPASSQVLRAAVPIHLKKENYSGTTTTRQEDPSVYIYGFFGD
                     ATSVSSKAVCKTAGTTLTRLLKVGTSTPLTVTDNNSAEPSNLLDTTLGFINLRGGVSL
                     GSTGPCVTQTSITTNLFETALGFKANMLENGDDNSADFKLPFQLPTSSSNSGNSALAS
                     TIIDADNISVSGTLLPGVASVLDNFVVYKAINLGTNFHYNSSFASCEALPGMGFSLVG
                     NTAVSGSTYQATLPLTDAEATGGAVFALCPTKAGVSMGLGVWLYASYMRYTGGCSGCG
                     GPSIATKLSVVTPQAYGGSTIANSTCTPAYVEGRDASNNLAQVPAGTTFTLISSNGEA
                     TLYTNSYCSTPVSASTSMNSMQYPFYIRRTVAGSASSVITVSGNSSLIGNSTTVNFID
                     PLTLTSATKKIKIIAPPSINAYMCIPITFESWYDDGTTAYMADFGYTSYTLPTIAGTS
                     YYYSYDWACSYSTTTNLSLGSTNGVSNQAFVKYTGAGTSLNLQPTSASPVYDGTYVGG
                     NNITAVPAGTAVAVRLSMSNSFGAESCQSVNLQVVDSMNNPSPTPQAITLNFTFNGSA
                     TPPANAGFYADPSCSTGINPAIAVSDTQASNLYFRWPTSGVLAIDATIATGPAGISFQ
                     PYTTTVGPTVVGSIVAYPSNGVTFAWPNTFAGLAMPIQKGQPFNITLEARSPLGNLVM
                     GFNDTNAGPNGVNLQDSYSSLNCGAISWSVGMGNVTCSVPNSPDYFASIMDGLKTGGG
                     ATIYPSFYGTPNVRVTRAAEEANTSTYLISKPFSYYANTCQPLMVTRGNYLGIHFISS
                     RVTSGMSYNANLTYSNGVAGFYSDSSCGTSITQTSLGAGQSTAIVYVKMDGTTPANYL
                     AMQADSNGVTDLGNTGLSFGVNAAPSTFTQYNISMSAYQFYHSCSPVMVVRADANGAA
                     VAAGGSENATFASNFAGSWYSDASCTTGLSGDSTTFNSGDAARLVYFRPDITTLSSGN
                     ITVSNGSQTGAISGINVQP"
     gene            complement(9527..12166)
                     /locus_tag="VIG33_08210"
     CDS             complement(9527..12166)
                     /locus_tag="VIG33_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEY8270859.1"
                     /translation="MKTERISLLIGFIITLIIFVFSLKFYEYKNTPAVLRADNPLLEA
                     FEVLDLRFNDFKYKLRQPVQSEAPVALIALDDASLREIGRWPWSRDLVSEMTQKLIDN
                     GVRSVGFDVIFSEPEKGFLEADTNFGKMIEKNQDKVILGTFSENLYAYQPYQDLCVAE
                     AFLKNGGDQLAKINPKFAIDEAGNLFDDLNWAPLFGLLFDNVQAQVEAEILKTFGKKS
                     ATEITEFQKNNLSSQKSQALFDYCKTWLTDKDVFLEADTIKKIEPLYLKLIADTKELS
                     PLSFSAFIKRIKESYKHHPIPQYGEWTPNVPVLQNPATFTASFIAKPDSDGYVRRYPM
                     YYRSGNKLGSSFIPSLTLQSYLLSGPLRAEVKMGVNSKGEKKIDEFNIYNVDTTPETK
                     IATLPVDKSGEILINFYGRQMSLPYVSAKELFNDKPTIRVQRGVKTAEGKQIQILEKE
                     FDKKEFFKNRSVLVGATAVGVYDLRNTPIDTNYPGPELHLTMLANLLDNNFLISWGKE
                     ASAMPWVLLVLGLALTCAWAYVDSLVSFAVFAGVMLAGLALDIWLFLSRKMMLHTFLP
                     VVLIMICFFSIQVYRYFTEEKKKRELKSTFSKYVSPAVVDELLKDAENLKLGGRKEYM
                     TVFFSDVRGFTTISEKLAPEELSRVLNLYLTPMTELIFKNNGTLDKYIGDAIMAFFGA
                     PVKHPNHAKEACRCALQNLAKLKELQKEFESQGLPYIDIGIGINTGAMSVGNMGSNIV
                     QNYTVMGDSVNLAARLEGINKEYGTRIIISQFTYEEVKDAFTAREVDRVRVKGKYEPV
                     RIFELVCEGEATDKVAEKLKLFNQGYGLYHEKKFTEALLLFKQVLEVLEDPVSKLYVD
                     RCQNYIDIAPPQDWDGVCVMKTK"
     gene            12463..12538
                     /locus_tag="VIG33_08215"
     tRNA            12463..12538
                     /locus_tag="VIG33_08215"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12496..12498,aa:Asn,seq:gtt)
     gene            13007..13405
                     /locus_tag="VIG33_08220"
     CDS             13007..13405
                     /locus_tag="VIG33_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270860.1"
                     /translation="MSNIVDLEKLFHRSKDRIRDLGEVFTPDSYVEEMLDLLAKDKRG
                     LWSDEEISFFEPCSGHGNIVLPIYKRRLDGIYKKVVSQGGGAGFERGQFHRFTGPMGT
                     IVTALKWDQRLKCQARSSALMVNQTDAIHG"
BASE COUNT         4004 a         2750 c         3060 g         3650 t
ORIGIN      
        1 aaaaaaactg aatctcaaag agtgggaacg ggcggaacct aaggctgccg caaaggttaa
       61 acgaagaaat gcctctttgc gaaacaaaaa aatcaaaacc ggaaatatta aagtccccga
      121 ttcggcattc aaagatgaaa acataactgc acatatttca atcaaacttc cattaaccct
      181 attcaaagag ctaaggcgct taagtttaaa tgagaagtat gatgggaaat acaaggtcct
      241 cattatcaat gttctaacaa aatatattaa gaaggcatcg aagcgaaaat agaattaggt
      301 cgatacgctt attgaattca atggcccggc cggtcggggt ggtaacgtca attggaagcc
      361 cccccacaga ttgagacagc tataaaacgt ataaaatcaa tagtactaag tagctgaaaa
      421 tagaggacta atttatttta ttgacaaaaa ctttagttat agctaaagta gccgataagt
      481 gggaggtagg cttcgcaaaa tcccctttat tctaagaggt aagaggtaag aggaaatgca
      541 aacgccgcca accagccctg agtataaggt aagttccgta gaaggcttgc catttctgca
      601 aggggggatg aaggcgattt gttttttaga acataaagaa aacaagtttg taaatgcaaa
      661 gacgatgttt tctaaatcga aagatgatgt tcagaaggcc ataagatatg gcattcagca
      721 gtggcttcaa ggcgttccag cgcccaaacg tcatcacggt tggaatcaat ctcaacatgg
      781 aggggcctac accaattgtt atgtgttcaa acacgtggca aagggagtga gattttacgg
      841 atgtttacat catcctgtcg cgaatggacg cgcgctgatt tgttgtgtaa ttcattacgc
      901 gataaaaaga gaagatgaag ttgatacata tattttggac gatattaacc aatttctagc
      961 taaagatgag tttaaggccg ctctggaggg cttttggaat gaacttcaga gtaaacaggg
     1021 agacgtgcaa tgaaaaaatc taaaggaaaa gaaacttatg aaaagaactg gactgaatct
     1081 gatattggaa actttctcta ttacgtaggt gctgactttg tagaccagtt gcaagcttct
     1141 tttgacactt tgggaattac tcagcgtagt tttgctcaaa agctcaacgt gtccgaaggt
     1201 agaatttcgc aggttttcaa tgatcctgga aatcttactt tagatactat gataaagtgg
     1261 ggtcgagctg cgggattgaa gaccggagtt attttatata acgacggtga cgtgaaaaac
     1321 gtgcgtggac ctttgagtgg gagtattttt attgagtgtt ggaaaagatt ggggtctccg
     1381 attgaaatgc ccaatacgca aagcaacttt cagggcagct tcaggagtca atacgataac
     1441 cctaggcaaa atactcccga tgataaagca gtctgcgaaa tgcagactgc tttggtaaga
     1501 acctgttctg gtgggcaagc taccttagtg aagagatttt tatcaaagca gtactgcaat
     1561 gaacctcaga gtgaagagtt ttacaccgtt cagtagataa gaaaagagtg ctttttatga
     1621 tgatgatagt ttctgtacca cctaaggaat tgagcgatgc tggtgttatt gccaaatcgg
     1681 ttaaccttgg atcgatcaga cttcgctctg cccatgtaga tgatgctaac tacgagttgt
     1741 ttttggatcc taatgatact agtgagatca cactgaatat cgattacaaa tctgagatga
     1801 aagagcttat tgaaggaaaa tctctaactt cttttgttaa agttgaagcg aaactattga
     1861 aaaaagaggc gttcattcga tatatggtgc aataccaagt ggattacacg ttacctagcg
     1921 tgccttttcc agaaaatctt tcccgcgaaa tgtttaatgc attcgctagg cataatggtc
     1981 ttttaaatag ttggccttat atccgatctc aaatcagcaa tttatcaaat gatgccggat
     2041 ttcctgtcat gctaccgttg ttgaaaataa ctgctcaaaa agatgaagcc atcaatagtg
     2101 aagtggtaaa aaccaactaa gccgtggttg tcatttttta acacgtaatc ttatttgtag
     2161 gtacagatgt aggttgaatc ccaacctgaa acataaattg ctattattac agggttttta
     2221 aaaaaggatt ggcggaaagg gcgggatttg aacccgcggt agaccttttg agtctacgag
     2281 cgtttagcaa acgcttggtt tcagccactc acccaccttt ccgcacgaaa attggatcag
     2341 ctttttaagg cagactccaa gaaactgcca tagccccccc ttataatcaa gcttagaccc
     2401 aattcttttt gcaaaatgcg taaagaacct tgattttttt ggttcagggt tctaaaacaa
     2461 gcgtctcttt cttgagtatt ttcttacgtg cagtcgtagc tcagctggat agagtacctg
     2521 actacgaatc aggtggtcgc aggttcgaat cctgccgtct gcaccaatct tttcattaaa
     2581 aatcgtcgaa atgaaccaat ttaatccatt ttaagttctc gaaatcaata tattttttta
     2641 ttttttctta actcattttg tgatgctcat ttcgactcag acttgagtct aaaaatgatc
     2701 tatttctaag gtgtttattc gttctggccg tagactcgtt tatcctatta ggagcgaggg
     2761 atttatgaag aacgtatttt ttggcgtaat tgcattttgc tttgtatttg cacttgaagc
     2821 acaggctttg gccgatcaaa tgtatggtgt tttaatggtc gtcaaaggct ctgtaaaaat
     2881 ccagaatacc agtaagcagt cctctgatgc gaaagtgggg ggcaaggttc ttgagggcga
     2941 aaccattatt actggtgcag atgcgcgcgc taaagtcgtg atgtctgacc gcaatgtcat
     3001 caatgtgacc ccgaacactc aggtcactat tacttcttat aaaaacgatt ccgctaccgg
     3061 cgaaaaaaat gtcgaattga atctattgaa gggaaaagtt cgcaataatg tggagcagac
     3121 ctatgatggc gagaaaagta aatttctcat taaaactcct acagctgttg cgggcgtgag
     3181 gggaactcaa tttctcgcgg gctttgatcc gggcactcgt atgacctcga ttgtaacctt
     3241 taaaggttcg gtttccttgg cttcattagg agccaacgga aaaatcatcg gtgtgccggt
     3301 tacagtaaaa aaaggcgaaa tgaccaccgc cgcccccaac gctcctccag aagcgccgaa
     3361 agctgttcct aaagaagatc tgaagaaaat ggatggggat tctaccgcat ctttacaaaa
     3421 taaagagtct aaagaagtga aagagtcaaa agattctaag gagcctaaag aagcgaagga
     3481 ctctaaggac tcaaaagagg cttctaatgg caaagagcaa aagcgagatg ttgcttctga
     3541 tgcacctgca gctaaagaac catcagctgc tcaaccaaca atgatagata gaagcgatat
     3601 agatccaaat caagcaacga ctgtcaatat ccctacgacc acttcccaac ctacaccgcc
     3661 ccctaagtcg acccctcctc ctcctatttc cacgccttct cccttggttc gaccaggaaa
     3721 aagtaatgtt aatgttcgac cggtaataca gaattagaaa gctgctaaga ttatgaaaag
     3781 aactgcgttt attttaatgg ctttgtgtct gggtgctatt agttatgcta aggaaaccaa
     3841 gatttcgcag tctcaagcga tccttgtaga acttcgtgat ccggcaggcg agcaagaaga
     3901 gcgacttatt cgcgtaaaag aagtcgagtc aaataaggtg acccagctca ctctacaaaa
     3961 agaggcctca tctccgcagg tatggaatgg gtcgttcatt attcaatttt ttgttggaga
     4021 taattccgca aagacattgg atttttatga tcctacaaat agcccctatt acgcttatat
     4081 ttctgacggc ggtggaaaat ctgttcagaa aattattcta tttaaaacgc cggaagaact
     4141 gtcacttttc gaagtgacta tggctgagga aaaacaaaaa gccgcggaaa aacaagaaaa
     4201 gcaaattgct aagcagatcc aagttgtagc gcctaaagga gctaaagccc ccataagcgc
     4261 tgaaaaatta aatcagttgg tgcgccagca gggacgcttg caagagacca ctcaaatgaa
     4321 cattgaagaa gctcaggcaa aaaaacgtat ggctttgctg gagcagcagc agaaaatgtc
     4381 tgaggaagct aagcaaaaaa agaaaaaaga agcggcagaa agcgcaaaga aagctgatgc
     4441 cttttatgct tcacaaaagt attctcaagc tgaaaaactc tatgccacag cctctgaact
     4501 tgatccagaa gccgaggctt atttttaccg ctatggtgta agtctttata aagtgggaaa
     4561 ctataataaa tcgctggcgg tgttatcaat ggctgatgtt tccggcgata attcggtcga
     4621 aaaagactac tatgtggctc tcaatcacat gaaactaaaa gactacgata aggcacggaa
     4681 ggaatttgtg gaaatccgcg aggaaaactc tccggagctg agtccgatgg cttcctttta
     4741 tgcgggcaat attgagtacc agcagcagaa atttcctgaa gctcgtaaga gtatggaata
     4801 tgtgatcgat aattctaagg agcctagtct tgataaatcc gcagaggata tgcttgagca
     4861 aattgatcgt atggagagtt tttacgaaag caaaaaagaa aaatatcgat ttaaaattta
     4921 tagcggatta atttacgata ctaatattct gaatgtggct gaaaataacg tatctaccga
     4981 cgtgaaagca tggcgattgt cttatggtgc aagtgttttg ggtatgatac atagaacgat
     5041 gacctcggat ttcggagctg agcttgccgt cgcggattat tatagcatga attccacctt
     5101 gaacaacgat gggaccgtgc aggctgcaga tgctttggaa atggatctca atttacccta
     5161 tcatcaagaa atcaagttga ataaaaatca aatgagcttg gaagtgatac cgagctataa
     5221 aaatatttat atggctactg ccggtggctc gcgcgctctg gccattaaga caaccgcgtt
     5281 gtcgacaaca ctctcagcac ctattaaaag tgatttgttt ttggcgacga agttggatct
     5341 ttcatctgat caatccctcg tggccactag cgttggggat gatgatcttt caggtcctaa
     5401 atatggactg acgtttacgc caagcaagct tttagatctt aaaggcgaaa aaatattatc
     5461 gggcgaactt agttatctta aaacccagtc aaatggaatt aataatcgaa gtgctaaaac
     5521 aggcctcgca atatcttacg ggttcccggg ggtctcaaag ggaaccagca gtcttcgcct
     5581 ggattatacc agccaggcct acgccgaggc aaccactcca cgcacagata cgaacatgat
     5641 actcactgga tcttatacca aagactttaa taaaagatgg aatatgcttt taagccttca
     5701 atacacgagt gctttatctg gtgtagagtc ttacaactac aacaaatttt tggtcaccag
     5761 tcttttcacg tatacaacga gtattctgca gaagtaaaaa agacttctag ttgatagaag
     5821 ccttcgcttt aaacttaatt atggttgaac attaataccg gagattgccc ccgtttggct
     5881 tccgttggag acggtaatat tgccacttga aagggtagtg atatctggcc tgaaatacac
     5941 aagcctcgcg gcatctccgc tattaaaggt cgtggaatcc ccagagaggc ctgtcgtgca
     6001 agaggcatcc gaataccaac ttcctgcgaa atttgaggcg aaagttgcat tctctgagcc
     6061 tcctgctgcc acggcggcac catttgcatc agctcggacc accattacag gggaacaaga
     6121 gtgataaaac tgatatgctg acattgaaat attatattga gtgaatgtac taggtgccgc
     6181 atttactccg aaactcaggc ccgtgtttcc gagatcagta acgccattgg aatcagcttg
     6241 catagcaaga taatttgctg gagttgtacc atccattttt acatagacaa tagctgtcga
     6301 ctgaccggct cccaatgaag tctgagtgat ggaagtaccg cagcttgagt ctgagtaaaa
     6361 gcctgcaaca ccattagagt aagtcaaatt agcgttgtaa gacatgcctg aggtcacccg
     6421 agaggaaata aaatggatac ccaaataatt tcctcgagtc accatcaatg gctgacaagt
     6481 gttcgcgtaa tagctaaatg gtttggagat gagataggta gaggtgttcg cttcctctgc
     6541 cgctcgagtg actcggacat tgggagttcc gtaaaaactt ggatatattg ttgctccgcc
     6601 cccggttttt aacccatcca ttatcgacgc aaaatagtcg ggcgagtttg gcacggaaca
     6661 agtgacgtta cccataccta cgctccatga aatggcgccg caattgagag agctatacga
     6721 atcttggaga ttaactccat tcggacccgc atttgtgtca ttaaagccca taactagatt
     6781 tcccagtgga gatcgcgctt caagagtaat attaaatggc tgacctttct ggatcggcat
     6841 tgctaggcct gcaaatgtat ttggccacgc gaaagtcaca ccattggagg gataagccac
     6901 aattgatccc acaactgtag gtcccacagt cgtggtataa ggctgaaaac ttattccagc
     6961 aggaccggtc gctattgtag catcgatagc gagaacccct gatgttggcc atcgaaaata
     7021 gagatttgag gcctgggtgt ccgacactgc aattgccgga ttgatccctg tagaacaaga
     7081 ggggtctgca taaaatcctg cgtttgcagg aggtgttgcc gagccattga aggtgaaatt
     7141 gagagtgata gcttgcggtg tcggacttgg gttgttcata gagtctacta cttggagatt
     7201 aacgctctgg caactttcgg ctccgaaaga gttggacatt gaaagtcgaa ctgctacagc
     7261 ggttcctgct ggaaccgcgg tgatgttgtt tccgccaaca taggtgccgt cgtaaactgg
     7321 ggaagcgctg gtgggctgga ggttgaggct agttcccgca cccgtgtatt ttacaaatgc
     7381 ttgatttgaa acaccattag tcgaacccag tgataaattg gttgtggttg aatatgagca
     7441 ggcccaatca taactgtaat agtaagacgt cccagcaatc gtaggtaaag tataagaagt
     7501 atacccaaaa tctgccatat aggcggttgt gccgtcatca taccaacttt caaaagttat
     7561 cggaatgcac atataggcat tgattgaagg cggcgcgatg attttgattt tcttagttgc
     7621 tgaggtgagt gtcagggggt caataaaatt gactgttgtg ctattgccga ttaaagaact
     7681 attcccggaa actgtgataa cgcttgaagc ggagccagcg actgttcttc ttatgtagaa
     7741 aggatattgc attgagttca tggaagttga agcagagaca ggagtgctac agtaagaatt
     7801 cgtatagagt gtagcctctc cattagagga tatgagcgtg aaggttgttc ctgccgggac
     7861 ttgtgccaga ttgtttgagg catctctacc ttcaacataa gcaggcgtgc aggttgaatt
     7921 ggcgattgtt gatccaccat aagcttgcgg ggtgacgaca cttaatttag ttgcaatgct
     7981 ggggcctccg cagccactgc agcccccagt atatcgcata taactggcat agagccagac
     8041 gccaagaccc atggaaacac cagcctttgt tggacaaagc gcaaaaacag cgccgccagt
     8101 ggcttcagcg tccgtcaagg gaagtgttgc ctggtatgtt gatccgctca cagcggtatt
     8161 gcctaccagg gaaaatccca ttccaggtag agcttcacaa gaagcaaagg aagaattgta
     8221 gtggaagtta gtgccgagat tgatcgcttt atagacgaca aagttatcta agacactggc
     8281 aactcctggc aagagagtcc ctgaaacgga aatattatca gcgtctataa ttgttgaggc
     8341 aagggcactg tttccactgt tactgcttga agtaggcagc tgaaatggca acttaaaatc
     8401 ggcgctatta tcatcaccgt tttcgagcat attggccttg aatcctaaag ccgtctcaaa
     8461 aagatttgtc gtgatactgg tttgagttac acagggacca gtggacccaa ggcttactcc
     8521 gccgcggagg ttgataaaac ctaatgttgt atccagtaaa ttacttggct ccgcgctgtt
     8581 gttatcagtc acagtgagtg gcgtagaggt gccaaccttc agcaaacggg tcagagtggt
     8641 tcctgcagtt ttacagactg ctttggagct gactgaggtg gcatcaccaa aaaaaccata
     8701 gatgtaaacg cttggatctt cttgccgagt ggtggttgtt ccagagtaat tttccttttt
     8761 caagtgaata ggtaccgcag ctctcaggac ttgactggaa gccggaaaag aagtttccga
     8821 taagtccacg gggccgtccc ataaaaattt accatcctcc attcgataac ccaatttagc
     8881 tccgataagg ccaaaaaatt gaaaccatcc gctgatcatc atggctctct ccacaatcat
     8941 cggagttttt ccgcctgggg ggacatagac aatatcgata cggctggttg gcccatccgt
     9001 tgcattagag acattagtgg aattagaggc actagagagg tatcgaccct gaattctccc
     9061 tgatatgata gtagcacccg ctcccactga agtcacaacg acaggtacat cttcggaagc
     9121 ggccgccaga gttttaatga tatccccata gtaaaatttc atagctccgc caccactatt
     9181 ggaagaggag gaatcaccat aaacagcaag aacttgaatt aagcgatcag ggccctgcgg
     9241 aatatcaaga gcaaaagaag aaggtgaggt cgccgccgtc gctgagccct tgctgtcgag
     9301 gtcccaattg aaaacgatgg gagctgggat tccgcttcca gtgatattaa cggaaacatg
     9361 aattagagta tcaaaagact gagcgttctg aggcaaagaa aaggaaatcg ttgatctagt
     9421 ttctttcgct gctcgcgtac aagagatgaa gcctacagat accatcattg ctgccagcaa
     9481 taacttattg aatcgatttg aaattctcat gacatctcct tcgcttttat tttgttttca
     9541 tgacacagac tccatcccag tcctgagggg gggctatatc gatatagttc tggcaccgat
     9601 ctacatagag ttttgagaca ggatcttcta acacttccag aacctgttta aagaggagca
     9661 aagcctcggt aaattttttc tcatgataaa gtccatatcc ctgattaaac aattttagtt
     9721 tttcagcgac cttatctgta gcctcgccct cacaaacaag ttcgaaaatc cgcacgggtt
     9781 catattttcc ttttacgcga acgcgatcga cttcgcgggc ggtgaaggca tctttgactt
     9841 cttcataggt gaactgactg ataataatac gtgtgccgta ttctttatta ataccctcta
     9901 ggcgggccgc taaattcact gagtccccca tgaccgtata gttttgaact atgttagaac
     9961 ccatattgcc gacgctcatc gcacccgtat taataccaat tccgatatct atgtaaggga
    10021 gtccctggga ctcaaattct ttttgtaatt ctttgagttt cgccagattt tgcaaagcac
    10081 agcggcaggc ctcctttgca tgattcggat gttttaccgg agctccaaag aatgccataa
    10141 tggcatcgcc aatatattta tctaaagtcc cgttattttt aaaaatcagt tcggtcatcg
    10201 gcgtaagata aagatttaag actcgagata gttcttcagg cgccagcttt tctgaaatcg
    10261 tcgtgaaacc ccgtacgtcc gaaaagaaca ctgtcatgta ttcttttcga cctccgagtt
    10321 ttaaattttc agcgtctttg aggagttcat caacaactgc gggagaaaca tattttgaaa
    10381 aggtagattt aagttcacgc ttcttctttt cttcggtgaa gtatctatag acctgaattg
    10441 agaagaagca gatcataatt agcacaactg gcagaaaagt atggagcatc atttttcgac
    10501 ttaagaaaag ccaaatatcg agagcgaggc cagccagcat gactccagca aaaacagcaa
    10561 aagacaccaa ggaatccaca taagcccaag cgcaggtcag ggcaagtcct agtaccaaga
    10621 gaacccaagg catggcactt gcttccttgc cccacgaaat caggaaattg ttatccaata
    10681 aattggccaa cattgtgaga tgaagttctg gtcccggata atttgtatcg ataggcgtgt
    10741 tacgaaggtc gtaaacgcct acagcggtcg ctcccacaag tacactgcgg tttttaaaga
    10801 actccttttt gtcgaattct ttttctaaaa tttgtatctg cttcccttca gctgttttga
    10861 ctcccctttg tacacgaatt gtgggcttgt cattaaagag ctcttttgcg gaaacataag
    10921 gtaaagacat ttgtcgtcca taaaagttaa ttaggatttc cccagactta tcaacaggga
    10981 gtgtggcgat ttttgtctct ggcgtagtat cgacattgta aatgttaaac tcgtcaattt
    11041 tcttttcacc cttggagttg actcccattt ttacttcggc tcgaagagga cctgaaagca
    11101 aatagctttg cagagtcaga gagggaatga aactggatcc taatttattt ccggagcgat
    11161 agtacatggg atagcgacgt acatatccat cagagtctgg ttttgcaata aagctcgcgg
    11221 tgaaagtagc tggattctgc aagacgggaa cattcggcgt ccattcacca tattgaggaa
    11281 tgggatggtg tttataactt tctttgattc ttttaatgaa agcagagaaa gagagaggac
    11341 tgagttcttt agtgtcagcg atcaatttca aatacagggg ttctattttt ttaatggtat
    11401 cagcttctag gaagacatct ttgtcagtca gccaagtttt gcagtaatca aaaagtgcct
    11461 gggatttttg cgaagacaaa ttgttttttt gaaattcggt aatctcagtg gctgattttt
    11521 tgccaaatgt tttaagaatt tcggcttcga cctgcgcttg aacgttatca aataatagtc
    11581 caaaaagagg agcccaattt aaatcatcaa ataggttacc ggcttcgtcg atcgcaaatt
    11641 tgggattgat tttcgcaagt tgatcgcctc catttttaag aaaagcctca gcgacgcaaa
    11701 ggtcttggta gggttggtag gcgtaaaggt tttcgctgaa agttccaaga atgactttgt
    11761 cctgattttt ttcgatcatt ttaccgaaat tcgtatcggc ttcgagaaag cctttttcgg
    11821 gttccgaaaa aatcacgtcg aatcctacgg atcgcacgcc gttatcaata agcttttggg
    11881 tcatttcact gacgaggtca cggctccagg gccagcgtcc aatttcgcga agcgaagcat
    11941 cgtctaaggc aattaaagca acaggggcct ctgactgtac cggttggcgg agcttgtatt
    12001 taaaatcgtt gaaacgaaga tcgagaactt cgaaagcctc caatagagga ttatcggcac
    12061 gcaggactgc cggcgtattt ttgtattcat aaaattttaa tgaaaataca aaaatgatga
    12121 gggtaataat aaaaccgata agtaggctga ttctttctgt tttcataggt tatatgtcgg
    12181 caacttcaat acaaacccaa gaccagataa aaaggaccac catggacttt tgccaaggat
    12241 ttagttaggc tctatttatg gacaaagtat ttttgcttaa aattatgaca atggtcctag
    12301 gtcttttcct ggttctttat gcgatgcgcg agctgcgcca accttcactc atccaacggg
    12361 caaataacaa cagccccctt tttttacttt tcggcaatgg gaacaaatag aaaaaatttg
    12421 actttatagg tcgtcggccc taatttggcc cttccgacgc gttcagaagt agctcagtcg
    12481 gtagagcaag cggctgttaa ccgctgggtc gggggttcga gtccctcctt ctgagccaat
    12541 tttatctcta tcaacccaaa taattgggta agcaaaaaaa caaacgtaat aacaaacagt
    12601 tacccgagag agggtttcta agttcacttt tccaatatca cagtaaatca ctcgaaataa
    12661 ccacatttgc agtcattttg tagtttttga aaactacaga tttttgaaaa actacaaaag
    12721 gcagaattag cggtaatagt tggctcgacc gtattcaaag agactccagt gggattcgat
    12781 ttagtcatca aaaatggcat ctttttttga acatgagtta tcaatacttg tgaacgactc
    12841 acgttagaca actttggtcg agtggacaga caaaagggcg gcattttaca caggatagaa
    12901 ggatagattt tttgaataga cagggtttct tgaggactta gctaaagcaa aaagcaaagt
    12961 gtcccgaaat gataaatgga aaagtgtcat ttctagggag ccatggatga gcaacattgt
    13021 tgatcttgaa aaattatttc atcgaagcaa agatcgcatc agagatttgg gtgaagtgtt
    13081 cacgccagat tcctatgtcg aagaaatgtt agaccttctc gcaaaagata aacgaggtct
    13141 ttggtcagac gaagagatat ctttttttga accctgctca ggccacggaa atatcgtcct
    13201 accaatttac aaaagacgac ttgatggcat ttataaaaaa gtcgtttctc aaggtggagg
    13261 cgccggtttt gaaaggggcc aattccaccg gtttaccggt cccatgggga ccatagtcac
    13321 cgctttgaaa tgggaccagc gcctaaaatg ccaagcaaga tcctcggcct tgatggtgaa
    13381 tcaaaccgat gctatccacg gctaagcgga tggaggtgcc atttttctct tggcaccatc
    13441 gctagagaga ttgtttattc cgcc
//
LOCUS       DATBTE010000224         4723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1396520, whole genome shotgun sequence.
ACCESSION   DATBTE010000224 DATBTE010000000
VERSION     DATBTE010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396520"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..561)
                     /locus_tag="VIG33_08225"
     CDS             complement(154..561)
                     /locus_tag="VIG33_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8270861.1"
                     /translation="MKSMPAIEKYMTTSPHSIGADQPLIKAEKMMREFNIRHLPVLDG
                     GKLIGILSDRDVKFIESFKDVDPQTVTVSEACTEEPYIVSPKAPLNEVCSEMAQHKYG
                     SVLVMDNNKLVGIFTWIDALEALNTLLETRLKH"
     gene            complement(736..2154)
                     /locus_tag="VIG33_08230"
     CDS             complement(736..2154)
                     /locus_tag="VIG33_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEY8270862.1"
                     /translation="MEKTPTGDLLKDLENVRLALDQSAIVAITDPQGTIIQVNDKFCE
                     ISKYSRNELIGQNHRLINSGYHSPKFFTHLWKTISNGQKWEGDIRNRSKYGQYYWVHT
                     VIIPFKDAQGKIKEYVSLRYDITQKKQTEENLQNLFDSAFEGLLIYDLSGRVVWRNLK
                     LAEVLGFSFPANEDVYFRNLFGKDTPLFQVGEFKIHRDSNQGSQVFELSCKNYSYQGH
                     RAYLVATRDVSEKEKYEAQILQQDRMASIGLLASGLAHEIGTPLGVMRGRAELLSLQK
                     SIEPAVRQNADIIIRQIDRISELIKSLLTLARGEGSATLNLVEINSIFDDVLKLLQYE
                     SQKYNIKVENTIDDPIIALAVPTSLFQVLLNLFVNSFHAIQERQSKEPGLVGKVILSA
                     KKENDFWWLRIQDNGCGISQENIKKIFSPFFTTKDVGIGTGLGLAISFKMLTSWNGGI
                     EVESSEGEGTLFSIKLRNKKIS"
     gene            2251..3693
                     /locus_tag="VIG33_08235"
     CDS             2251..3693
                     /locus_tag="VIG33_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8270863.1"
                     /translation="MDKKSRILVVDDDPGMGALLQDFLTPQGYTVETYTSAVEAYNHL
                     QKADRWHLEQIHLIITDLRMPGMDGLSFIEKCKVLEPSIPIILITAFGSIDSAIEATK
                     KGAFSYIVKPFKLSEMDLTVERALQFRSLNKQNKILRGDHRSAHSYAGIIGKSAAMHS
                     VFDLIQRVSGAMANVLIQGESGTGKELVAKAIHSQSSRAGKPFVAINCTAIPENLLES
                     ELFGHVKGSFTGAIANKKGLFEEAQGGTLFLDEIGDMDLALQAKLLRVLQDRKIRAVG
                     DTHDKPIDVRIIAATHKDLKAGIRDGRFREDLYYRLSVIPVVLPPLRHRLEDIPILAH
                     VFLDKYSSLNGTRVETFSKEAMQTLMSLSWDGNVRELENVIERAVVLCRGAQIEVGDL
                     PRGENSNSEMVFAQASEELPTLEDFEKRYIKYVLDRTGGRKEKAAHILGVNRRTLYRK
                     EREYGWVVGEDPLAEDCYHVAAGAINHREN"
     gene            3828..4220
                     /locus_tag="VIG33_08240"
     CDS             3828..4220
                     /locus_tag="VIG33_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270864.1"
                     /translation="MKNFKLLLLSSLLVIGVSTQASPAAVEKEVQIGIGDVYVPGGFD
                     SNSSAFVVANGYFPNSCYRWNRSEVKHIDTFTHEVKSIASVSQGMCLMVMVPFTKEVS
                     LGKFESGKHTLRFLNGDGTYLEKSMSIE"
     gene            4274..>4723
                     /locus_tag="VIG33_08245"
     CDS             4274..>4723
                     /locus_tag="VIG33_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270865.1"
                     /translation="MKNVLILFVILGCFSAFFSGCESRTGFQEIEWSKKSGKPLPAEL
                     KKINLKTDIQNQTRFEKIEFQSQQVQGIPLENAFVKKIYSQTGELISLSGVVDNRAAA
                     NKADIENMKQQQSEVISRLRSRYPELAKVNDKIITFEQPELIFEKKGK"
BASE COUNT         1350 a          931 c         1077 g         1365 t
ORIGIN      
        1 ggccgctacg atggcggcca ggaaggcctt ctcatggaag gaggcactct ttagttatcc
       61 ctcactgctg tgagcaaact ctttctaatt cgcaaaatcc tttaagggat ttcattatca
      121 acaatatgga gggccagttt ctaatgtttg agtctagtgt ttgagtcgcg tttctaacag
      181 agtattaagt gcttcaagcg catcaatcca agtaaagatt ccaaccagtt tattgttatc
      241 cattaccaag acagaaccat atttatgctg ggccatttca ctgcagactt cattgagcgg
      301 agctttgggc gaaacgatat agggttcttc agtgcaagct tctgaaacag tcacggtttg
      361 ggggtccaca tctttaaagc tctcgataaa ttttacatcg cgatcagaaa gtataccaat
      421 caactttccg ccgtctaata cgggaaggtg gcgtatattg aactcacgca tcatcttttc
      481 cgccttgatg aggggttgat cagcgccaat cgaatgtgga gaggtggtca tatatttttc
      541 aattgctggc atgcttttca tagggactcc cattttaagt ttccctttgc tagggcaaaa
      601 aaagtgccaa cggaaaagta tttttattgg acctaagagg atagttgaga caatttatct
      661 caaatatatt ttatatttga gatatcaagt ctcataaata ctaagagggg tccttttctt
      721 gtttgcagga cttcgtcagg atattttttt attgcgaagc tttatgctaa aaagggtgcc
      781 ttcaccttca gagctttcga cttcaatgcc gccattccag cttgtaagca tcttaaatga
      841 aatggctaag cccaatcccg tgccgattcc cacatctttt gtggtgaaaa aagggctgaa
      901 gatttttttt atattttctt gagaaatgcc gcagccattg tcttgaatgc gaagccacca
      961 aaaatcattc tcttttttag cactaagaat aactttgccg acgagaccgg gctctttgga
     1021 ctggcgctct tgaatggcat gaaaagaatt tacaaataaa ttcaaaagca cttgaaaaag
     1081 tgaggtgggg actgccaggg cgatgatcgg atcgtcaatg gtattttcga ctttaatatt
     1141 gtatttttgg ctttcatatt ggagaagctt taaaacatca tcaaaaatag aatttatttc
     1201 cactaaattt agagttgctg aaccttctcc tcgggctaaa gtgagaagag acttgattaa
     1261 ctctgagatt cgatcaattt gccggataat gatgtccgca ttctggcgca cggcgggttc
     1321 aatacttttt tgtaaactta aaagctcagc ccgtccccgc atgactccta ggggcgttcc
     1381 gatttcatgg gcaagtcctg aagccagaag accaatggag gccatccgat cctgttgcag
     1441 aatttgcgct tcgtattttt ctttctcgga gacatcgcgg gtggccacta agtaagcgcg
     1501 gtggccttga taagaataat ttttacaact cagttcgaag acctggcttc cctggtttga
     1561 atcacggtga attttgaatt ctcccacctg aaaaaggggg gtgtctttgc cgaagaggtt
     1621 gcgaaaataa acatcttcgt tggcaggaaa ggagaagcct agaacctcag cgagtttcag
     1681 atttctccag accactctgc cgctcaggtc ataaatcaaa agaccttcaa aggcggaatc
     1741 aaaaagattt tgcaaatttt cttcagtttg tttcttttga gtgatgtcat agcgaagtga
     1801 gacatactct ttgattttgc cttgggcgtc tttaaaagga ataatcacgg tatggaccca
     1861 ataatattgt ccatacttgc tccgattgcg gatatcgcct tcccattttt gcccattcga
     1921 aatggttttc cacagatgag tgaaaaattt cggagaatga tagccggaat taataagacg
     1981 atggttttgg ccaataagtt catttcggct gtatttggaa atttcacaaa acttatcatt
     2041 gacctgaata atagtccctt gaggatctgt aatcgcaacg atggcggatt ggtctaaagc
     2101 aagacgtaca ttttccagat cttttaagag gtccccggta ggtgtttttt ccatgaatag
     2161 atcttaaaca atagacacct tgaattcaat ctgttactcg aagatctcga gaagaagttc
     2221 gttagtggag gctatttatg gataatattt atggataaga agtcacgaat cttggtagtg
     2281 gatgatgatc caggtatggg agctttgctc caagactttt taactcctca aggatacacg
     2341 gtcgagacct acacgtccgc agtcgaagct tataatcact tgcaaaaagc cgaccgctgg
     2401 cacttggaac aaattcattt gatcatcacc gatctgcgca tgccaggaat ggatggtctg
     2461 agttttattg aaaaatgcaa ggtcttggaa cccagcattc ccattattct gatcactgct
     2521 tttggctcta ttgactccgc cattgaagcc accaaaaaag gcgcctttag ttatatcgtg
     2581 aaacctttta agttgagtga aatggatctt acagtcgaaa gagctttgca gtttcgcagt
     2641 ctgaataaac agaataaaat actgagagga gatcatcgtt cagctcattc ctacgccggg
     2701 attataggta aaagtgcggc aatgcactcg gtgtttgacc tcatccaacg ggtttccgga
     2761 gccatggcga atgttttgat ccaaggagag agcggcacgg gaaaagaact cgtggccaag
     2821 gccattcatt cacaaagctc tagggccggt aaaccctttg tggctattaa ttgcacggcc
     2881 attcctgaga atcttttaga gtccgaactt ttcggccatg tgaaaggctc ttttacggga
     2941 gccattgcca ataaaaaagg tctctttgag gaagcgcaag gcgggacttt atttttagat
     3001 gagattggag atatggattt ggccttacaa gccaagttgt tacgagtgtt gcaagacagg
     3061 aaaattcgcg cggtgggcga cacccacgat aaaccaatcg atgtgcgaat tatcgcagcc
     3121 actcataaag atctaaaggc cgggattcgt gacggccgat ttcgcgaaga tctctattat
     3181 agactcagcg tgattcctgt ggttctgcct cctttgcgtc accgtctgga ggatattcct
     3241 attttggccc atgtctttct cgacaaatac agttcgttga atggaactcg agtggagact
     3301 ttcagtaaag aggcgatgca aacattgatg agcttatctt gggatggaaa tgtgcgcgaa
     3361 ctcgaaaatg tgattgagcg ggcagtggtt ctttgtcgtg gcgcacaaat tgaagtgggt
     3421 gatctgcccc gcggtgaaaa ctccaattct gaaatggttt ttgctcaagc ctccgaagaa
     3481 ctccctaccc ttgaagattt tgaaaagcgt tacataaaat atgttctgga taggacaggg
     3541 ggtcgaaaag agaaggccgc ccatatcctc ggtgtgaatc gtcgcacttt atataggaaa
     3601 gagcgagagt atggttgggt ggtaggggag gaccctcttg cggaggattg ttaccacgtt
     3661 gcggcagggg caattaatca cagggaaaac tgattcattg ggaagatttg tctcaagaaa
     3721 atcttttaat tgaattaagt ggatttaaga cttggcctta tacttgcttt agtattagat
     3781 aaactcgtca acaaacaaca aacgcgagca cagcaaggga gatgcaagtg aaaaatttta
     3841 aattacttct tttaagtagt cttttagtta tcggtgtttc aacacaggcc agtccagcgg
     3901 ctgttgaaaa agaagtccaa attggaattg gcgatgtcta tgttccaggt ggattcgaca
     3961 gtaattctag cgcctttgtg gttgctaacg gttattttcc aaatagctgc taccgttgga
     4021 atcgttcaga agtaaagcac atcgatactt ttactcatga agtaaaatcg attgcttcag
     4081 taagccaagg tatgtgtctc atggtgatgg ttcctttcac taaagaagtc agcctcggta
     4141 aattcgaaag cggaaaacac actttgcgct tcttgaatgg cgatggaaca tatctagaga
     4201 aaagtatgtc catcgaataa tctaaggatt ctcaagcccg attgtaaact cgggcttttt
     4261 cttggagcca ggtatgaaaa acgttctaat tttgtttgtg atcttaggtt gtttcagtgc
     4321 ttttttcagt gggtgtgaat cccgaactgg ttttcaagaa attgaatggt cgaagaaatc
     4381 aggcaaaccg cttcctgccg agttaaaaaa aataaattta aaaacagata ttcaaaacca
     4441 gacaagattc gaaaaaattg aatttcagtc tcagcaagtc cagggtattc cgctggaaaa
     4501 cgctttcgtt aaaaagattt acagccagac aggcgaactc atttctcttt caggtgtagt
     4561 ggataacaga gccgccgcaa ataaagcaga tattgaaaac atgaaacagc agcaatcaga
     4621 agttatttct cgactgcgca gccgttatcc tgaactggcg aaagtgaatg ataagatcat
     4681 cacttttgag caaccagaat tgatttttga aaaaaaaggc aaa
//
LOCUS       DATBTE010000225         2938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1327451, whole genome shotgun sequence.
ACCESSION   DATBTE010000225 DATBTE010000000
VERSION     DATBTE010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1327451"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VIG33_08250"
     CDS             <1..1099
                     /locus_tag="VIG33_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY8270866.1"
                     /translation="VRGNKFPDNMEMEWDGKPLKTQIHVGIDKELQKEAEKLLKTYKP
                     DYAAIFMMDAVTGRVLAMTSFQKDDPNAENWNLKSNFPAASVFKVVTATAAMDKAGVS
                     PQHKINFNGGNYTLYRKNVMSDKITKWTRVITLKEAFARSINTAFGRLSLETLHPVDI
                     NDYATRFMFNQEIPSDFPVEMGVAYVPPERGFELTEVASGYNKTNRMSPVQGAMIAAA
                     VANEGKMVIPYLVQNITDETGQNIYQAESLDNGHIMTAESANKVRELMGETLTAGTSR
                     KSFRKLVHNKKFKTIEMGGKTGHLTAENPRGRVDWFVGYAMDETNKIAIAAVSINKEK
                     WTVKSSELGQNMFKKYFGPIIDERMISSSGQ"
     gene            complement(1655..2422)
                     /locus_tag="VIG33_08255"
     CDS             complement(1655..2422)
                     /locus_tag="VIG33_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY8270867.1"
                     /translation="MKIISWNLNGIRACHKKGLLDFVKREKPEVFCVQETKAYIDQVE
                     DEAKHLGYKYFYWSSAVRKGYSGVATFTDVEPLHNAIGWGQNEYTSEGRIVITDHGMF
                     DLYNIYFPNGGSGDERHSFKQKFLKDLNLHLKEKLESGREIIVVGDYNVAHKEIDIYD
                     PKRLSKESGFLPEERAWFDDFLALGFVDTFRKFHPQEAHRYSWWSYRELARISNRGWR
                     IDYICVSKGLEKNLVSADILDTVEGSDHCPVVAMLKF"
     gene            2567..>2938
                     /locus_tag="VIG33_08260"
     CDS             2567..>2938
                     /locus_tag="VIG33_08260"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY8270868.1"
                     /translation="MIYQPAPQFTTQAVFDSGEIKNISLLDYKGKWVVLFFYPLDFTF
                     VCPTELTQFREYLPEFTKNNCAVLGASVDSVHSHKRWLRDDLGNLGFPLIADLTKRIS
                     RDYGVLIEEKGIATRGTFIIDP"
BASE COUNT          895 a          659 c          609 g          775 t
ORIGIN      
        1 tgttcgaggc aataaatttc ctgacaatat ggaaatggag tgggatggaa agcccttaaa
       61 aactcaaatc catgtgggaa tagataaaga gttacaaaaa gaagcggaaa aactcttgaa
      121 aacatataaa ccagattacg ccgctatctt tatgatggat gccgtcactg gccgagtgct
      181 cgcgatgacg agctttcaaa aagatgatcc aaatgctgag aattggaatt taaaatccaa
      241 ttttccggcc gcttccgttt ttaaggttgt caccgccaca gcggcgatgg acaaggctgg
      301 agtttcccct caacacaaaa ttaactttaa tggcggcaac tacacccttt accgtaaaaa
      361 cgtaatgtcc gacaaaataa ccaagtggac tcgcgtgatc actttaaaag aggcttttgc
      421 cagatccatc aatacagcct ttggacgatt aagtttagag actcttcacc cggttgatat
      481 caatgactat gcaactcgct ttatgttcaa ccaggaaatc ccttccgatt ttccggtgga
      541 aatgggcgtg gcttacgtcc caccagaaag aggatttgaa cttactgaag tggcttcggg
      601 ttataacaaa accaatcgca tgagcccggt tcaaggcgcc atgattgcgg cggctgtggc
      661 gaatgagggc aagatggtga ttccttatct tgtacaaaac atcacggatg agaccggaca
      721 gaatatttat caagcggaat ctttggataa tgggcatatc atgaccgcag agtctgcaaa
      781 taaagttcgt gaattgatgg gagagaccct gactgcggga acttctcgga agtcttttag
      841 aaaactagtt cataataaaa aattcaagac gatcgaaatg ggtggaaaaa caggccatct
      901 caccgcggaa aatccgcgag gtcgtgtgga ttggttcgtg ggttacgcca tggatgaaac
      961 caataagata gccattgctg cggtttcaat taataaagaa aaatggactg tgaagtcctc
     1021 agagctaggt caaaatatgt ttaagaaata tttcggtcct atcattgatg agcgtatgat
     1081 ctcttcttct ggccagtagg cttcctaatc ttctaatgag ggatctcttt ttctcgtggc
     1141 ttcctcttca attttagtat gagtgatttc aagtggggtg acatccacta ctttcgcctc
     1201 ccgctcagtc gaggcgtctt gttcaaacgg aaagggaccc tgaggtctgc ttccaaaccc
     1261 tcctgctccc ctaccaaagc gaacgaatga aaactgcgac tttgacagat acttggccaa
     1321 atgtttgaaa aggaaggtct taaatatgaa aatcgaagca tgacgaaaga ttggcaaaat
     1381 caaaaaaagc gccaacactc gactaagaaa cagtggaata ataagcaaaa tgctgcctat
     1441 cacgaaggct ccgcgatgaa gcattttgtc tgcaggcatc tcgccttgaa ctaagccttt
     1501 gctcatcctc gaaaataaca gaattcccaa aaaactcgga aacaaatagc agaaaaacac
     1561 atcccaaaaa ttatagaaat gcacgaaggt tgaaaaaatt aagacttcga taagtacaag
     1621 gggaagggga ataaaaaaca tatcagcctc tctactaaaa ttttagcatc gctacaaccg
     1681 ggcagtgatc tgagccctca acggtatcta agatatccgc tgacactaaa ttcttctcga
     1741 gccctttgga gacacaaata taatcgattc tccaaccacg gttagaaatc cgtgcaagtt
     1801 cgcgatagga ccaccacgaa tagcggtgag cctcctgcgg atgaaacttt cggaacgtat
     1861 ccacaaaacc caaagccaag aagtcatcga accaggctcg ctcttctggt aagaagccac
     1921 tctctttcga aagccttttg gggtcataaa tatcaatttc cttgtgggcc acattataat
     1981 caccgaccac gataatctct cgccctgact ccagcttttc tttcagatgc aaattcaagt
     2041 ctttcaaaaa tttctgtttg aaactatgcc gttcatcacc cgatcctccg ttcggaaagt
     2101 atatattata gagatcgaac atgccatgat cagtaattac gattcttcct tcagaggtgt
     2161 actcattctg tccccagcct atggcattat gcaatggttc cacatcagtg aaagtggcta
     2221 ctccagaata gcccttgcga accgctgagg accaataaaa gtatttatag cccaaatgtt
     2281 tcgcttcatc ttctacctgg tcaatataag ccttggtttc ttgaacacag aaaacctctg
     2341 gtttttctcg tttaacgaaa tcaagaagtc cttttttgtg acaggcgcgt atgccgttca
     2401 aattccagga aatgattttc atattgacct cacgtctcca ttgctgcaaa aaagttgttg
     2461 caacaatagg gtttaggaca cgaagaacaa gccttaacat ttagcattaa acgttaagaa
     2521 tactcatata gccatgcccc ccaagccagg agatgcgaca gtgccaatga tttatcaacc
     2581 cgccccccaa ttcactacgc aagccgtttt tgatagcggt gagattaaaa acatttctct
     2641 tttagactat aaagggaaat gggtggtttt attcttctat cctttagatt ttaccttcgt
     2701 ttgtcctacg gaattaactc agttccgtga atatcttcct gaattcacca aaaacaattg
     2761 cgcggtttta ggtgctagcg tggattcagt tcattctcac aaaagatggc tgcgcgacga
     2821 cctgggcaat ctggggtttc ctctgattgc agatctcaca aaacggatat cccgcgatta
     2881 tggcgtttta atcgaggaaa aaggcattgc aacacgcggc acttttatta tcgatcct
//
LOCUS       DATBTE010000226        15258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2216584, whole genome shotgun sequence.
ACCESSION   DATBTE010000226 DATBTE010000000
VERSION     DATBTE010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 15258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15258
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2216584"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VIG33_08265"
     CDS             complement(<1..186)
                     /locus_tag="VIG33_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270869.1"
                     /translation="MNNIAEKIDAFLEIEYPGATSTIYRDLSLNFKKVLEESSLEPQE
                     RFTNLLSIAVALENKAMV"
     gene            complement(326..859)
                     /locus_tag="VIG33_08270"
     CDS             complement(326..859)
                     /locus_tag="VIG33_08270"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY8270870.1"
                     /translation="MLGIGDKFPEFKLQACVSREKGKEFAEISNANFKKSWAVLFFWP
                     LDFTFVCPTEIAEFNKAYKDFANREAKLFGLSADSHFTHLAWRENHADLKDLQFPMIA
                     DYNKTLTNTLGIASKENGAPLRATYIVDPEGIIRWVSVNDLSVGRNVKEVVRTLDALQ
                     TDELCPCNWEKGQKTLG"
     gene            complement(880..1314)
                     /locus_tag="VIG33_08275"
     CDS             complement(880..1314)
                     /locus_tag="VIG33_08275"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEY8270871.1"
                     /translation="MKYKPSYRASLSTEEIQAKLQAAGVQPTLQRIAICKYVLCEADH
                     PTAEQVKEWAEENLEKISQATVYNTLNTLVEAKLLRIFKFPHSEKVIYDNNIHNHYHF
                     LDESSGNLYDIETKDVSINMEIPSKFTVNSMEVVFKGQIKAT"
     gene            complement(1427..2476)
                     /locus_tag="VIG33_08280"
     CDS             complement(1427..2476)
                     /locus_tag="VIG33_08280"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEY8270872.1"
                     /translation="MMNKIALVIFTLGFATSCAYLASVTPPSKPPTPPLPAVTAEIVE
                     SKEIKKSESLQPRGIDLGATVKVVAFSSSANQNLPQPIWKSIALNIPDLFLFMGDNVQ
                     FSKTEPSSLMEQYHKLDRIPEYHAFREKVPFMATWNDQDYGPKEFLRYWTYVANSISL
                     GQHSVYHAKIIGPKKKQVQIIMLDTRSFRTKADDAKATLLGETQWGWLEEQLKRPAQV
                     RLIVSSIPLIAFEKSANKAANQWSNFPQERQRFFDLLKKTNARNVVVLSGNRHQSSIA
                     KISLKNWGILFDITASPINEPLAIAEEDSSFEGKPSAEESFGLAQIDWHDKKMSLQIR
                     DINNKIMNSVSFKIK"
     gene            2734..3648
                     /locus_tag="VIG33_08285"
     CDS             2734..3648
                     /locus_tag="VIG33_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270873.1"
                     /translation="MAAKNFLFILSLIFSAPFAKADSPCEKSPGALVIAHGSHHHGPH
                     KHALGDRDPWNDTITEAVGNAKERLEMPIELAFGMWDKASFEAGVNKLALQGVCELRI
                     IPLFISSKSEVIDIQKYMFGINEKNEFPIHIEKVVLPQQITEIKFGKALDDHEYVSQI
                     LFERLSEISLNPVSEGIIFIAHGPYGDAYETSWLDLLKLHADRIQQRFTQVHAESFYD
                     LSYFTLRDDSPSAIRDNRTQQIRNKVQELNNKSITPIISPVLIAEGGIESGLRERLKG
                     LNYKIQTKFLLPNPKMVDWIVERAQNPY"
     gene            complement(3706..4872)
                     /locus_tag="VIG33_08290"
     CDS             complement(3706..4872)
                     /locus_tag="VIG33_08290"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate synthase/citrate
                     synthase"
                     /protein_id="HEY8270874.1"
                     /translation="MAEYINPDYVAEPEKMNVKKGLEGAVIDTSSVSKVNAETNSLVY
                     RGYPVQDLAENCSFEEVAYLMYNSELPNATQLAAFTQKERGYRDISTTLLNVIKALPA
                     KCHPMDSIRTAVSFQGCEDARIWDASPATNMDKAIQLLAKIPTMVAADYRFKKGLDFI
                     PPKASLSIAENFFHMCFGKIPQKEIVKAFDASLILYAEHSFNASTFTARVVTSTQSDL
                     YSATVAGIGALKGPLHGGANEQVMHMMIEITDPAKAEQWMIDALAQKRKVMGFGHRVY
                     KSGDSRVPTMKKYAQVVADVMGEQKWMKMYNSLEKVMVEKKKIYPNLDFPAGPAYYMM
                     GFEIDFFTPIFVMARTTGWSAHIMEQTANNRIIRPLSEYVGHAQRKVTPLNERA"
     gene            complement(5092..5487)
                     /locus_tag="VIG33_08295"
     CDS             complement(5092..5487)
                     /locus_tag="VIG33_08295"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY8270875.1"
                     /translation="MMKGKKIIFSALPLLIAGCASQPASLNKDDVRPVILSHLKEVRI
                     CYEATLERTPKSEGKLVVSWQINKEGKATNYTAIKESSTIFDQRLIQCLGDTLSTWQF
                     PIPPDDAVAEITYPFFFRPHSEEQSGPAK"
     gene            complement(5508..6410)
                     /gene="prpB"
                     /locus_tag="VIG33_08300"
     CDS             complement(5508..6410)
                     /gene="prpB"
                     /locus_tag="VIG33_08300"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEY8270876.1"
                     /translation="MLFPEITPAQKRKNFRDQLKTGKLLQFPGSWSPLVSMAIEKEGF
                     DGVYISGSVLSNDLGYPDIGLTSLTEVASRGRQIARTTQLPTLIDIDTGFGEPMSATR
                     TVQEMIEMGLAGCHIEDQINPKRCGHLDGKGLVGKEEMVRKVAAAARGKKLDENFLLI
                     ARTDARAVDGLDKAIDRAKAYIDAGADCIFTEALENEKEFETFRKAVSVPLLANMTEF
                     GKGRLYTYQQLSDLGYNIVIYPVTTFRLAMGATVAGLAEIKAKGTQEGLLDKMQHRKD
                     LYTLTRYEEYNSFDTSIFNFTVKK"
     gene            complement(6411..7919)
                     /locus_tag="VIG33_08305"
     CDS             complement(6411..7919)
                     /locus_tag="VIG33_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY8270877.1"
                     /translation="MKKHSVRVYPSKEPLNRKDQLAWKMAEVAADAAPIKADVLDMVI
                     NRIIDNASVAMASANRRPVASARAMAIAHPRANGATVFGMPNDQKFDCEWAAWANGTA
                     VRELDYHDTFLAADYSHPGDNIPPILAVAQQMNKGGKDLLRGIVTGYELHVDLVKAIC
                     LHEFKKDHIAHLCPAQAAGIGTLLGLPTETIFQAIQQAVHVSFTTRQSRKGEISSWKA
                     YAPAHAGKLAVEAVDRVMRGEGAPSPIYEGEDSVIAYMLSGKNGKYEIPLPEGGEEKR
                     AILETYTKEHSAEYQSQALIDLAARMKTKITNFDDITKIVIHTSHHTHYVIGTGANDP
                     QKMDPTASRETLDHSIMYIFAVALQDGTWHHVKSYEPSRAQRPDTVSLWHKISTVEDA
                     KWTEAYHNSDPDKKRFGGRVEIFFKNGSTLVDELGVANAHPAGAKPFKRADYIRKFEM
                     LTEEIITKEERHRFITLVENLADLTPAQVQQLNVQIPLDRLVNNKRDTRGIF"
     gene            8139..8468
                     /locus_tag="VIG33_08310"
     CDS             8139..8468
                     /locus_tag="VIG33_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270878.1"
                     /translation="MKPIVVGLLLALAGVQVQAHGRLEAYEICTSMTFTSGKNKCISA
                     LRNYEYFDPWAIDVCIGMSFDAGKLECIANIGDKIFDAYDVIACEKENSFDSGKNRCL
                     KAVGQPF"
     gene            complement(8566..9255)
                     /locus_tag="VIG33_08315"
     CDS             complement(8566..9255)
                     /locus_tag="VIG33_08315"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEY8270879.1"
                     /translation="MRARLILILTMCLFLMRTNSFAATGTPLDDILTPDELATELGIN
                     QPTDLSPQIEPLTADQVSDLYGINIYREFPVVIVISKGSQTATVYHYGTQVNRFLIST
                     GREQMETAKSGRRYFTTTPTGWFSPKRYVATYWSDTWEARMDYAIFFNGGVALHATTP
                     DHYKDLGRKASGGCVRMHRTNAIWFWNLSQGYRYASVPYFTRGGQLLRNRDGSIRRHM
                     GNGTLVIVTSY"
     gene            complement(9368..10564)
                     /locus_tag="VIG33_08320"
     CDS             complement(9368..10564)
                     /locus_tag="VIG33_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8270880.1"
                     /translation="MFSRQEKILLGILATIQFSSIVDFMIIMPLGPQLMRIFGISPHQ
                     FGLLVSSYTFSAGLSGFAAAFFMDKFDRKKNLLFFFIGFSLGTIACSISPNYETLLVA
                     RGLTGVFGGVLSSLVMAIVSDAISYERRGSAMGVVMTSFSMASVLGVPLSLYLANVWG
                     WHAPFLILGILSLLLCAVIWQAVPSMKAHLSHQIQEPIHRTITRILHNKNQINALIFM
                     AAVIFGHFAIIPFMSPSLISNAGLTEAELPLMYMCGGFFTIFTSPFIGRLTDRFGKHR
                     VFFWGALATMIPYFILTHLGPTPLFLVLLYTTIMFMVSGGRMIPASTMVSGTALPQTR
                     GSFMSIVSCVQQLASAAASYIAGIVVTRDSTGHLLNYEMVGYMSIATTLIAIYLSSRI
                     RTVEMT"
     gene            complement(10637..11101)
                     /locus_tag="VIG33_08325"
     CDS             complement(10637..11101)
                     /locus_tag="VIG33_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEY8270881.1"
                     /translation="MQRKAQAIWRGSLQLGKGEISTLSGALKGVPYTFNTRFSETPGT
                     NPEELIAAAHASCFAMACSAGFSDKGFEPENLEVTANVTLQKLGSAWAVTSSLLQLKA
                     QVAGVPLDQFTSIVEEAKNNCPISKLLNLNIALEAHLEEAPPPRPWAPPPPQ"
     gene            complement(11270..13330)
                     /locus_tag="VIG33_08330"
     CDS             complement(11270..13330)
                     /locus_tag="VIG33_08330"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY8270882.1"
                     /translation="MTSNYLLLIPAIAGAVGLVLALVLYFRVKSQPTGNETMNRIAQY
                     IREGSMAFLSREYKVLAGYAVVVSLALGFSLGWLSAGCFVLGAFLSLLAGFFGMKAAT
                     YANVRTTQAAASGSKSKALLVALDGGAVMGLSVAGLGLLGLGILFQIYNGTPEVGTVL
                     HSFAVGASSIALFARIGGGIYTKAADVGADIAGKVIEGIPEDDPRNPGVVADNVGDNV
                     GDVAGMGADIYESMVAAIVAAMAIALTAPAETLQALTSNAAILRTSAIFLPLGLSALG
                     MIISILVIFITRLFSNSAPATVLRNALIVPPILLTIASYVLMPMYDLNQTVTVALAAG
                     AIGGALIGLITEYYTAARPIRLVAEASLTGAGTNVIRGLAIGMESVAIPMLIVVIAAF
                     VADRVLGLYGIALAAVGMLAGTAVVMTVDAYGPIADNAGGISEMSGLGKNVRDITDEL
                     DAVGNTTAAIGKGFAIGSAILTVVALFTAFNMEVNHTRALAGLAKINLDLTSSGVLIG
                     LLIGAILPFLVGATTMTAVGKAAQKIVTEIGRQFKEIPGLLQGKADPEPAKIVDIATK
                     AALIEMIIPGMIAVLAPVAIGFLLGPTGLAGMLAGALAVGATMSLFMANAGGAWDNAK
                     KYIEKGKLPGHKKGSDAHKNAVVGDTVGDPFKDTSGPGVAILIKVMSVVSLLIAGLVA
                     TIGG"
     gene            complement(13531..14469)
                     /locus_tag="VIG33_08335"
     CDS             complement(13531..14469)
                     /locus_tag="VIG33_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270883.1"
                     /translation="MTEKILTKLTAEYDNADVPRRFQIQSEVTQDLDALAESVFNLFL
                     NKTPNLSAWTKNIQGCLNRFPSLNNKLVYSLNRKISNWLFTKMQNSHSAKMRALAKIF
                     TSGSRNLKFNTFSWLQQNEFFQEKGGYQFFQNWIFLDLERVSPEELLFVFVHELAHEL
                     DTTLKSNLDIFNNYKVTEIIYSTQNLTPANLDRIDHWLQASLQIHFLAEWRAWLFTYE
                     IYRDLINLDHIPPVDWLEKKIPDVAKLPAELLTSKVFNFLDKKFYDPTNEYPFDIPIL
                     KERLLSLRETLRNSQTLPQQDPCLANLTQNTCPSEK"
     gene            complement(14593..>15258)
                     /locus_tag="VIG33_08340"
     CDS             complement(14593..>15258)
                     /locus_tag="VIG33_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270884.1"
                     /translation="PSVAIESNFEVSLAKIRRLNKYCISKGLADVLRPLIDSEYRNEL
                     IEATKGHTYSDDSDWYEVRVNKSYFLASSCSIGRKNYDQFRSYGEDYYEPFIYIGLYK
                     TCKKENGFYGESNFVFNRQNKDHVDLMNSPELNDFPQLKFTKTQLKPIHFINGMPDKS
                     VEVANGITLITPKDMKDLEVLYQVYRSNDDPKNAALIESQLIFNSGSVVQCLMNGLTD
                     GTK"
BASE COUNT         4311 a         3355 c         3461 g         4131 t
ORIGIN      
        1 caccattgct ttattctcca gagccaccgc gatggagagt aaattagtaa agcgctcctg
       61 aggctcaagc gaggactcct ctaaaacttt tttgaagttc agagaaagat cgcggtaaat
      121 ggtgctggtg gctcccggat attctatttc caggaaagcg tcgatttttt ctgcgatgtt
      181 gttcataaat tcctcctgtt aaaaaaaggc atagaaaact ggagccttta aaaaaggctc
      241 taagcactgc tgataaagca aagcttagag cctcattatt gttttgaatc gggaatgtca
      301 gtgatctttt atagaccgtt gggaattaac ctaaagtttt ttggcctttt tcccagttgc
      361 aaggacaaag ctcatctgtt tgcaaagcat ctaaggtacg tacaacttct ttcacattgc
      421 gacccacaga caagtcattt acagaaaccc aacgaatgat tccttctggg tccacgatgt
      481 aggtcgcacg aagaggagcg ccattttctt tagaagcgat gcccaaggtg ttggttaaag
      541 tcttattgta gtccgcgatc atcgggaatt gaagatcctt aaggtctgcg tgattttctc
      601 tccaagccaa atgagtgaaa tgagagtccg cgcttaaacc aaataatttc gcttcacggt
      661 ttgcgaaatc tttgtaagct ttgttgaact ccgcaatttc agtcgggcaa acaaaagtga
      721 aatccaaagg ccagaagaac aaaacggccc aagatttttt gaagttagca ttgcttattt
      781 cagcaaactc tttgcctttt tcacgggaaa cacaggcttg taatttaaac tctggaaact
      841 tatcgccaat acctaacatg atatactcct ttgtaagaat taagtggcct ttatctggcc
      901 tttgaaaacg acttccatag aattcacagt gaactttgac ggaatttcca tattaataga
      961 aacatcttta gtttctatat cgtaaagatt cccggaactc tcatcaagaa aatggtaatg
     1021 attatggatg ttattatcat agatcacttt ctccgaatgt gggaatttga aaatccttaa
     1081 taatttagcc tcaaccaaag tgttcaaagt attgtacact gtggcttgac tgattttttc
     1141 aagattctct tcggcccatt ccttgacttg ttctgctgta ggatgatcgg cttcacacaa
     1201 aacatattta cagatagcaa ttcgttgcag agtgggttgc actccggccg cctgaagctt
     1261 ggcttgaatt tcctctgtac ttaaagaggc tctgtaagat ggtttgtatt tcatctctca
     1321 atccttcgaa ttctttgttt tgaactcttg gttagaatag ttctaaattt agaatgcgtc
     1381 aatagtaatt tagaactatt ctaaaaatat ttataagcat atgaaatcac ttaattttaa
     1441 atgatactga gttcattatt ttattgttta tatcccgaat ctgcaggctc attttcttat
     1501 catgccagtc aatttgggcc aggccgaagc tttcttcagc agagggtttt ccttcaaaag
     1561 agctgtcctc ttccgctatc gcgagcggct cattgatagg acttgccgtg atatcaaaca
     1621 agattcccca attttttaaa ctgatttttg cgatggagga ctgatggcga ttgccgctca
     1681 gtacaaccac gttgcgtgca ttggttttct ttagaagatc aaaaaaacgc tgacgttctt
     1741 gcgggaaatt agaccattgg tttgcagctt tattcgcact cttttcaaaa gcgatcagcg
     1801 gaatgctact cacaatgagc cgaacttgcg ccggacgctt gagttgttct tcgagccagc
     1861 cccactgagt ctcgcccaaa agggtcgctt ttgcatcgtc tgctttggtg cgaaaagagc
     1921 gagtatccaa catgatgatc tgcacttgtt tctttttcgg tccgataatt tttgcatgat
     1981 agacgctgtg ttgccctaag gagatagagt tcgccacata agtccaataa cgcaaaaatt
     2041 cttttggccc ataatcctga tcattccagg tagccatgaa cgggactttt tcgcggaagg
     2101 cgtgatactc aggaatgcga tccagtttat gatactgttc catcaaagaa cttggctcag
     2161 tctttgaaaa ttgaacgttg tcccccataa atagaaataa atcagggata ttcagagcga
     2221 tgcttttcca aataggttgc ggcaagttct gattggcgga ggaactaaaa gcgaccactt
     2281 tcactgtggc tccaaggtca atgccccggg gttgaagtga ctcactcttt ttaatttctt
     2341 tgctctctac gatttcagcc gtcactgccg gaagtggcgg tgttggtggc ttggaggggg
     2401 gcgttaccga tgctaggtag gcacaagaag ttgcaaatcc taaggtgaaa atcaccagtg
     2461 ctattttatt catcatccgt cctttggttg attgagttta tattcaatcg aaaggtggtg
     2521 gatgagtcaa aatctctctg ttactcgcca tatcacttcg cttaatcact tcacttacat
     2581 ttaggtttta tttcgtttgc gtttatagaa gcctctcctc ctttaattga tttagttttg
     2641 ctaaagaaag aaacttttca gaccaaaggg tctccctccc aaggttggat caaatggaat
     2701 gagataaaaa acaaatttaa gaagaggttt taaatggcag caaaaaattt tttattcata
     2761 ttaagtttaa ttttttcagc gccctttgca aaggctgaca gtccatgtga aaaatcaccc
     2821 ggtgcactgg ttatagcgca tggaagccat caccatggtc cccacaaaca cgctttaggc
     2881 gatcgagacc cttggaatga taccatcacc gaagccgtag ggaatgcaaa agaaaggctc
     2941 gagatgccca tcgagttggc cttcggaatg tgggacaagg cttccttcga agcgggagtg
     3001 aataagctgg ccttacaagg agtttgtgag cttcggatca ttccgctttt tatcagcagt
     3061 aaaagtgaag tgattgatat tcaaaagtat atgtttggaa tcaatgaaaa aaatgaattt
     3121 cctattcata ttgaaaaagt tgttctgcct cagcaaatca cagaaattaa atttggaaag
     3181 gctttggatg atcatgaata cgtcagtcag attctctttg aaagactctc cgaaatttca
     3241 ctcaatcccg tttctgaagg aattattttt atcgctcacg ggccctatgg ggacgcctat
     3301 gaaaccagtt ggctcgacct tctgaagctt cacgccgatc gcattcaaca aaggttcact
     3361 caggtccacg cagaatcttt ttatgatctt tcttacttta ctctcagaga cgattcgccc
     3421 tccgctatcc gcgacaatcg cacccaacag atcagaaata aagttcagga gctaaataat
     3481 aaatccatca ctcctatcat tagcccggta cttattgccg aaggtggaat tgaaagtggc
     3541 ctgagggagc ggctcaaggg tctaaattat aaaatccaaa ccaaatttct tctgccaaac
     3601 cctaaaatgg tggattggat tgtcgagcgc gcgcaaaacc cgtactaaaa tgaaagccct
     3661 ccggtcaagc aaaggaggac tttgtgaatt attttttaga aaaatttaag ctctttcatt
     3721 gagaggagtc acttttcgtt gtgcatggcc aacatattca ctgagtggac gaatgatacg
     3781 attgtttgcc gtctgctcca taatgtgcgc tgaccatccg gtcgtgcgcg ccatgacgaa
     3841 gatcggagtg aagaagtcga tttcaaaacc catcatatag tacgcaggtc ctgccgggaa
     3901 atcgagattc ggatagattt tctttttctc caccatcact ttttcaagtg agttgtacat
     3961 tttcatccat ttttgttcgc ccataacatc agctacaacc tgtgcatatt ttttcatcgt
     4021 aggcactcgg gagtcgccag atttatagac tcgatggcca aagcccatca ctttacgctt
     4081 ttgcgcgaga gcatcaatca tccactgctc tgctttcgca ggatcggtga tttcgatcat
     4141 catatgcatg acttgctcat tagctccgcc atgcaaaggc ccttttagag cgccaatacc
     4201 cgccaccgtc gctgagtaca gatccgattg agttgaggtt acaactctgg ccgtgaaagt
     4261 cgacgcattg aagctgtgtt cagcgtacag aatcaaagaa gcatcaaaag ccttcacgat
     4321 ttctttttgc ggaattttac cgaaacacat atgaaagaaa ttttcagcaa tgctcaggct
     4381 agctttcgga ggaataaaat ccaacccttt tttaaagcga taatccgccg caaccatggt
     4441 cggaatttta gcaagcaatt gaatcgcttt atccatattg gtagcagggc ttgcatccca
     4501 gatccgagca tcttcgcaac cttgaaagga tacggcagta cggatagaat ccattggatg
     4561 gcatttagca ggcaatgctt tgatcacgtt caacagagtc gtggaaatat cgcgatagcc
     4621 ccgttctttc tgagtaaaag ccgccagttg ggtcgcattc ggaagttcgc tattatacat
     4681 caaataagcc acttcttcga aagagcagtt ctcagccaag tcttgaacag ggtaaccacg
     4741 ataaaccagg gagttggttt ccgcattgac cttggacact gaagaagtat cgatcacagc
     4801 tccttcgagt ccttttttaa cattcatctt ctctggttca gccacataat ccggattgat
     4861 atactcagcc atggttctcc tcggtacgcc gtaccttttt cggatgcagc gtgctatttt
     4921 aagttgtgaa ttgtaccaag ctattgcgag gctaagattg agtcaaggtt cttgcggagt
     4981 gccattggcg agaggcacgt tcattttctc cgaaagtttt gattgcatga aaatcattgt
     5041 atcacaaaat tgcttccggt atggaagtta aaatgcagag aattggcgct cttacttagc
     5101 gggacctgat tgttcttctg aatgagggcg aaagaaaaaa ggatacgtga tttcagcaac
     5161 agcatcatcg ggaggaatag ggaactgcca cgtggaaagg gtgtcaccaa ggcattggat
     5221 gagtcgctga tcgaagattg ttgaggattc tttaatagct gtatagttgg ttgctttgcc
     5281 ttctttattg atctgccatg agacaacgag tttaccctca gacttagggg ttctttccag
     5341 agtcgcttca tagcagattc ggacttcttt taaatgacta aggattacag gtcgaacatc
     5401 gtctttattt aggctcgcag gctggctagc gcaaccagca atgagaagtg gcaatgcgga
     5461 aaaaataatt tttttgcctt tcatcattat tgagtccatt ccctgaatta tttcttaact
     5521 gtgaagttaa aaatgctcgt atcaaagcta ttgtactctt catagcgagt cagagtgtac
     5581 aagtccttac gatgctgcat tttatccaga agaccctctt gagttccctt ggctttgatt
     5641 tccgcaaggc cagccacggt ggcgcccatc gcaagacgga aagttgtcac tggatagatg
     5701 acaatattat agcctaagtc cgaaagctgc tgataggtgt acaagcggcc tttcccgaat
     5761 tctgtcatat tcgctagaag aggaacggag accgctttac ggaaagtttc aaattctttt
     5821 tcattctcga gggcttctgt gaaaatacaa tcagcgcctg catcgatata ggctttcgcg
     5881 cggtcgatgg ctttgtccaa gccatcgacc gcgcgggcat ctgtgcgggc gatcaaaaga
     5941 aaattctcat cgagtttttt accgcgagcc gcagccgcca cttttcgcac catctcttct
     6001 tttcccacga ggcccttgcc atcgagatgg ccgcaacgtt tggggttgat ttggtcttca
     6061 atgtggcaac cggcgaggcc catctcaatc atttcttgga cggtgcgagt cgcactcatg
     6121 ggttcgccga aaccggtatc tatatcgatg agggtgggca actgagtggt gcgagcgatt
     6181 tgtcgtccac ggctggccac ctccgtcaaa gaagttaatc cgatatcggg atagcccagg
     6241 tcattcgaaa gtacagatcc agaaatataa actccgtcaa agccttcttt ttcaatagcc
     6301 atactcacca agggtgacca ggatccgggg aactgcaaga gcttcccggt tttgagttga
     6361 tcacgaaaat ttttgcgttt ttgagcggga gtgatttctg gaaataacat ttagaatatt
     6421 cctcgagtgt cgcgtttgtt gtttacaagt ctgtccaagg gaatctgcac attgagttgc
     6481 tgaacttggg ctggagtcaa gtctgccaaa ttctcaacca aagtaatgaa acgatggcgt
     6541 tcttctttgg tgatgatttc ttcagtgagc atttcaaact tgcggatata atctgcacgt
     6601 ttgaaaggtt tcgcgccggc agggtgggca ttcgccactc ccaattcgtc cactaaagtc
     6661 gaaccatttt tgaagaagat ctccactcgg ccgccgaagc gttttttgtc tggatcagaa
     6721 ttatgataag cctcagtcca cttcgcgtct tctacggtgg aaattttatg ccacaaagac
     6781 acagtatcag gacgttgagc gcgggaaggt tcataggact tcacatggtg ccaagtccca
     6841 tcctgaagcg ccactgcgaa aatatacatg atggaatgat ccaaagtttc gcgggaagct
     6901 gttggatcca ttttttgtgg atcattggcg ccggtgccga tcacatagtg agtgtgatga
     6961 gatgtatgga tgacgatttt agtgatgtca tcgaaattgg taattttagt tttcatgcgc
     7021 gccgccaagt cgatcaaggc ttgagactga tactccgcag agtgctcttt ggtgtaggtc
     7081 tcaagaatag cgcgtttctc ctcgcctcct tcaggcaatg gaatttcata ttttccattt
     7141 ttgccagaaa gcatgtaagc gatcacagaa tcttcccctt cataaatcgg agacggagcg
     7201 ccttctccgc gcatcacgcg gtcgacggct tccacagcca atttcccagc atgagcaggg
     7261 gcataagcct tccaagaaga gatttcacct ttgcgagatt ggcgagtggt gaaagacaca
     7321 tgcacagctt gctgaatcgc ttgaaaaata gtttcagtcg gaagacccaa cagagttccg
     7381 atacctgctg cttgagcagg gcagaggtga gcaatatggt cttttttaaa ctcatgcaaa
     7441 caaatagctt tcaccaagtc gacgtgaagc tcataaccag tgacaatgcc gcgaagcaag
     7501 tctttgcctc ccttattcat ttgctgagcc accgccaata ttggggggat gttatcgcca
     7561 ggatgagagt aatccgctgc caagaaagta tcgtggtaat ccaattcgcg aacggcagtg
     7621 ccattggccc aggcggccca ttcgcagtca aatttttgat cgttaggcat tccaaagaca
     7681 gtcgctccgt tggcgcgagg atgggcaata gccatcgcgc gggcagaggc cacaggacga
     7741 cggttggccg atgccatggc cactgaagca ttgtcaataa tacgattgat caccatatcc
     7801 agaacatcag ctttgatggg agccgcatct gcggcaactt cagccatttt ccaggcaagc
     7861 tggtctttgc ggtttaaagg ctctttggag ggataaacgc gaacagagtg ttttttcata
     7921 atggattcct tttggtgttt aacaaatagc aaaaactaac gtcagacagc aaaagcgtca
     7981 agaaaggcct ctcaggaagc cctgaatcat cagaaaaagc cccgccaata agagaaaaat
     8041 atcctccgga tacaggcttt ttgaagataa gttagccaat ccggcatcac ccatacataa
     8101 ttataaatac aatgctcaaa acaaaatgag gagaaaacat gaaacctatc gttgtgggcc
     8161 ttctactggc tttagcagga gtgcaagtgc aagctcatgg gcgcttagaa gcctatgaga
     8221 tttgcaccag tatgacgttt acatccggca aaaataaatg tatttcggcg ctgagaaatt
     8281 acgagtattt tgatccctgg gctatcgatg tttgcatagg aatgagtttc gatgcgggca
     8341 agttggaatg cattgcgaac ataggagata aaatcttcga cgcctatgat gtcatcgctt
     8401 gcgaaaaaga aaatagtttt gattcaggta aaaaccgatg tctgaaagct gtaggtcagc
     8461 cgttttagat tcttatctat cgtccgagtc gaaaagctgt cgttttcagt catttcgacc
     8521 cgaatgaact attaaaaacc tacagcaaat tttgggatta tcctcttaat aagaggtgac
     8581 aatcaccagt gtgccattgc ccatatggcg tcttatgctg ccgtctctat ttctgaggag
     8641 ctgacctccg cgggtgaaat acggaacact ggcgtatcta taaccctgtg aaagattcca
     8701 gaaccatatg gcattggttc tatgcatgcg tacacagcct cccgaagcct tacgccccaa
     8761 atctttgtag tggtcgggag tggtcgcatg cagcgcgact cctccgttga aaaaaatcgc
     8821 ataatccatc cgcgcttccc aggtgtcaga ccaataggtg gcgacatagc gtttgggaga
     8881 aaaccatccc gtgggagtcg tggtgaaata ccgtcttccg gatttggccg tctccatttg
     8941 ttcccgtcct gtggaaatga gaaatctgtt tacttgggtg ccatagtgat aaacagtagc
     9001 tgtttgcgag cctttactga tcacgatcac cacgggaaat tcgcgataga tgttgattcc
     9061 atagagatca gaaacttgat cagccgtgag gggttctatt tgagggctga gatccgtagg
     9121 ttggttaata ccgagttcag tggctaactc atcgggtgtc agtatatcat ccaagggtgt
     9181 tccagtggcg gcgaatgaat tcgtgcgcat taaaaacagg cacatggtaa gaataagaat
     9241 taaacgtgct ctcatataaa aacctcctac gatttgttga ttgcagcagt gcaaccttta
     9301 gatgctaaag cagccttttc gtagaattgc gctctccatt ttatttaaag cggaagaaat
     9361 agaaatttta tgtcatttcc acggtgcgaa tacgacttga tagataaatt gcaatcaagg
     9421 tggttgcaat cgacatatat cccaccatct cataatttaa caaatgaccg gtcgagtccc
     9481 gagtgaccac aatgccagcg atataacttg cggccgccga cgccagttgc tggacacaac
     9541 tgacgatact catgaagctg ccgcgagttt gcggcaatgc tgtgccagac accatcgtcg
     9601 acgcgggaat catacgcccc ccggaaacca tgaacataat tgtagtataa agcaaaacta
     9661 agaagagcgg agtgggtccc agatgagtca aaataaaata cggaatcatt gtggctagag
     9721 ccccccagaa aaagacccgg tgcttgccga agcgatccgt cagtctgccg ataaacggtg
     9781 aggtgaaaat agtgaaaaag cctccgcaca tgtacatcaa gggcaactcg gcctccgtca
     9841 accccgcatt ggaaatcagg gagggtgaca taaaagggat gatggcaaaa tggccaaaga
     9901 taaccgccgc cataaaaatt agtgcattga tttgattttt attgtgcaaa atacgcgtga
     9961 tggtgcggtg aatgggttct tggatttgat gacttaaatg agccttcata gaaggcacgg
    10021 cctgccaaat aaccgcacag agaagtaagg ataaaattcc cagaatcaag aagggtgcat
    10081 gccatcccca gacattggct aaataaaggc tgaggggcac tccgaggacg gaggccatgg
    10141 aaaaagaagt catcaccaca cccatagcac tgccccggcg ttcataggaa atcgcatcgc
    10201 tcacgatggc catgaccagg gaactcagga ctccgccgaa aacaccggtc aaaccccgag
    10261 caacaagcag agtttcataa ttcggagaaa ttgaacacgc gatggtgccc agggaaaaac
    10321 caataaagaa aaataaaaga ttttttttac gatcgaactt atccataaaa aaagcggcgg
    10381 caaagccact gagtcccgct gaaaaggtgt aggaggacac caataaacca aattgatgag
    10441 gcgagatgcc aaaaattctc atgagctgag gtcccagtgg cattatgatc ataaaatcaa
    10501 cgatggagct aaactgaata gtagcgagaa tcccgagcag aatcttttct tggcgagaaa
    10561 acatgactaa atccttggct gatatcgttg aggccttagt ttatttgata gcgaaggatt
    10621 tgcctaggga tgctttttat tgtggaggag ggggggccca aggacgaggc ggcggagctt
    10681 cttccagatg agcttcaagg gcaatgttaa ggtttaataa cttagagata gggcaattat
    10741 tctttgcctc ttccacaatg gacgtgaact ggtctagggg gactccggca acctgggctt
    10801 tgagttgtaa aagggaagac gtcactgccc aagcgcttcc taatttttgc aaagtcacat
    10861 tcgccgtgac ttccaaattt tccggttcaa aacctttgtc tgaaaacccc gcggagcaag
    10921 ccatggcaaa acaactggcg tgggcggcgg caatcaactc ttcaggattg gtcccaggag
    10981 tttcactaaa gcgggtgtta aaagtgtagg gcacgccttt gagggctcca cttaaagttg
    11041 agatttcccc ctttcctaac tgtaaagatc cccgccaaat agcttgtgct ttccgttgca
    11101 taaacacccc cgatttgaat tcaatttaaa aaagttttct tggctcttga aagacaaatt
    11161 ctccgaaagt gcggaagaac agaaaggcga ggtgttggaa aacaaaaaaa cccccacaaa
    11221 acatgtgggg gttttttata actaaattct aagaaagaat tcgcagtctt tatccgccga
    11281 tggttgctac taatccagca atcaaaaggg aaacgacgct cattactttg atgaggattg
    11341 caacacctgg gccagaagtg tctttgaatg ggtcaccgac agtgtcgccg acaacggcgt
    11401 tcttatgggc atcagagcct tttttgtgac ctggaagttt tcctttttcg atatattttt
    11461 tagcgttgtc ccatgctcca ccggcgtttg ccatgaacaa agacattgtc gcacccacag
    11521 ccaaggcacc cgctagcatc cccgctaaac ctgtagggcc caacaagaaa ccaatagcca
    11581 caggagccaa aactgcgatc atgcctggaa tgatcatttc gatcagtgcg gctttggtgg
    11641 cgatgtccac gattttagca ggttcaggat ctgcttttcc ttgaagaaga cctgggattt
    11701 ctttgaattg acggccgatt tcagtcacga ttttttgtgc tgctttacca accgcagtca
    11761 ttgtggtcgc gcctaccaag aacggcaaga tcgcgccaat caacaaaccg atgaggacgc
    11821 cggaagaggt caaatccaaa ttgatttttg ccagacccgc aagagcacgg gtgtgattca
    11881 cttccatatt gaaagcggtg aaaagagcga ccacagtcaa gatagctgaa ccaattgcaa
    11941 aacctttacc gatcgccgca gtggtgtttc caaccgcatc gagttcatca gtgatgtcgc
    12001 gaacgttttt accaagacca gacatttcag agatcccgcc ggcattgtca gcgatagggc
    12061 cgtaagcatc cacggtcata accaccgcag ttccagccaa catacctact gcggcaagag
    12121 cgattccata aagacccaaa acgcgatccg ccacgaaagc ggcaatcacc acgatcaaca
    12181 tagggatcgc tacagattcc ataccgatag caagaccacg gatcacgttg gtgcctgcgc
    12241 cggtcaaaga agcttcagcc acaagtcgga taggacgagc tgctgtgtaa tactcagtga
    12301 tcaagccgat cagagcaccg ccaatcgcac ctgccgccaa agccacggtc actgtttggt
    12361 tcaaatcata cattggcatt aaaacgtacg aggcgatcgt gagcaagata ggtggaacga
    12421 tcaaggcatt acgcaaaact gtcgccggag ccgagttgct gaacagacgg gtgatgaaga
    12481 tcaccaaaat agagatgatc atacctaaag ccgaaagacc caaaggtaag aaaatcgcgg
    12541 acgttctcaa gatcgctgca ttagaagtca gagcttgaag agtttccgcg ggagcggtga
    12601 gggcaatggc catggcggca acaatggctg caaccataga ctcgtaaata tccgcaccca
    12661 taccggcaac gtcaccgaca ttgtctccta cgttgtcagc gaccacacct ggattgcgtg
    12721 ggtcatcttc aggaatgcct tcgatcactt taccagcgat atctgcaccc acgtcagcgg
    12781 ctttcgtata aattccgcct ccgatacgcg cgaacaacgc aatggaagaa gcgcccacgg
    12841 cgaaagaatg cagaacagtc ccaacttcag gagttccgtt atagatttga aaaagaatgc
    12901 cgagacccaa aaggccaaga cctgcgacgg aaagacccat caccgcgcca ccgtcaaggg
    12961 cgacaagaag agctttcgat ttagagccac tagctgcagc ttgagtggtg cgaacgttag
    13021 cataagtcgc tgctttcata ccgaagaagc cggcaagcag acttaaaaaa gctccgagga
    13081 cgaagcaacc tgcagaaagc caccccaatg aaaaacccag tgctaagctg acaaccacgg
    13141 cgtagccagc cagaactttg tactcgcggg agaggaaagc catggaacct tcacgaatgt
    13201 actgcgcgat gcggttcatt gtttcattac cagtaggttg agacttcaca cggaagtaca
    13261 aaaccagagc tagaacaaga cctacagctc cagcgattgc tgggattaac agcaaataat
    13321 tacttgtcat tttaaaaaac tccatcgttg aacttgcaca gttatttaag atttagtgaa
    13381 cagttagagg tacgaaaaac gtcgtattta gtcacgcgtt gcgtatcgcg ttattgcaaa
    13441 agctgcggtt agaagccgca gtgaccatga actgggcggt tggtcttgcc gtcgaaatta
    13501 gctttaaagg tggcttcgtg atgactttag ttacttctct gacggacaag tgttctgcgt
    13561 taaatttgcg aggcaagggt cctgttgcgg tagtgtctgg ctgtttctca aggtttctcg
    13621 caaagacaat aatctttctt taagaatggg aatatcaaaa gggtattcgt tggttggatc
    13681 gtaaaacttt ttgtctaaga aattaaagac cttactcgta agaagttcgg caggcagttt
    13741 tgcaacatct ggaattttct tttccaacca atcgacgggc gggatgtggt ctaaattaat
    13801 taaatctctg taaatctcat aggtaaagag ccaggctcgc cattctgcta aaaagtgaat
    13861 ctgcaacgaa gcctgaagcc aatggtctat tcgatctaaa tttgcaggag ttaaattttg
    13921 agttgaataa ataatttcgg tcaccttgta gttattaaaa atatccaaat tggacttcaa
    13981 tgtggtatcg agttcatgag ctaattcatg aacaaagaca aagagcagtt cctctggtga
    14041 aacccgctct aaatctaaga agatccagtt ttggaaaaat tgatatcctc ccttttcctg
    14101 gaaaaactca ttttgctgaa gccaagaaaa ggtattaaat ttcaaatttc gggatccaga
    14161 ggtaaaaatt ttggctaaag ctcgcatctt tgccgagtga gaattttgca ttttcgtaaa
    14221 tagccagttg gaaattttcc tattcaaact ataaacgagt ttattgttta agctaggaaa
    14281 gcgattcagg cagccttgaa tattctttgt ccacgcagac agattcggag ttttatttaa
    14341 aaacagatta aagactgatt cagcaagtgc atcgaggtct tgggtgacct cggactgaat
    14401 ttgaaagcgt cgtgggacat cggcattatc atattcagct gttaattttg ttaatatttt
    14461 ttcagtcatg ggatccgcag agcaagaatc tacgccacca aagctgaaac ttgagaaggt
    14521 taaaattaag agcgaaaaag taatttggta taaagtacta aatctcgtca taaattgaac
    14581 tttaggggag ttttatttgg tgccatcggt aagaccattc attaagcatt gcacgacgga
    14641 tcctgaatta aaaatcagtt gggattctat caatgctgca tttttcggat catcattaga
    14701 tcgataaact tgatagagaa cttctaggtc tttcatgtct tttggagtga ttagcgtaat
    14761 gccgtttgca acttcaactg atttatctgg catcccatta ataaaatgaa ttggttttaa
    14821 ttgtgttttt gtaaatttca gttggggaaa atcattaagt tcaggtgagt tcattaaatc
    14881 tacatgatct ttattttgtc tattgaaaac aaaattgctt tctccgtaga agccattctc
    14941 ttttttgcag gttttatata atcctatata aataaatggc tcatagtaat cttcaccata
    15001 tgatctaaat tgatcgtagt tcttcctgcc aatagagcag ctagaggcta gaaaataaga
    15061 tttgttcact ctaacttcat accaatcgga atcatctgag taagtatgac cttttgtcgc
    15121 ctcaatcaat tcgtttctat actcactgtc gatcagtggc cttagaacat ctgctaaacc
    15181 tttgctaata caatatttat taagacggcg gattttagct aagctcactt caaaattact
    15241 ctcaatcgca actgaggg
//
LOCUS       DATBTE010000227         1543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_507624, whole genome shotgun sequence.
ACCESSION   DATBTE010000227 DATBTE010000000
VERSION     DATBTE010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507624"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VIG33_08345"
     CDS             <1..652
                     /locus_tag="VIG33_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8270885.1"
                     /translation="NDVVTVDEISKTWEGESLLKNVSFTLKKGDKVVILGRNDLAKTL
                     LMEILAGELSADSGSCTWGITTSINYFPTDNSKYFKGSEETLVDWLRPYSKEKDETFL
                     RGFLGKMLFSGSDAMKKPNVLSGGEKVRCMFARMMLSGANILILDGPTNHLDLESISA
                     VNEGLSRFKGTVIFTSHDHELIQTVANRIIEIDQAVVYDNHISYEEYLSLKSQGKA"
     gene            728..967
                     /locus_tag="VIG33_08350"
     CDS             728..967
                     /locus_tag="VIG33_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270886.1"
                     /translation="MEDLPVESFIFSFSFGSLIAGFIFGVIGFWLLGHGRKKANNRNV
                     VIGLLLMMYPYFVRGAWLNWGIGFALCGYAYYTWD"
     gene            complement(975..1466)
                     /locus_tag="VIG33_08355"
     CDS             complement(975..1466)
                     /locus_tag="VIG33_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270887.1"
                     /translation="MKAPFCSICQKPKATLECGICKNAICKKCAQFIDEDTFSFLPHI
                     PVEFSTGTFCGPCFDFKVSPEIETYNQTMEAAKEIAVFFNDQGKETRLVKRLADPVQV
                     VECKDRDETVLRLAFFAAQGNYNGIVDVDVKSEKVKNGTYQTLRWRGTAIPANIDPKK
                     LNK"
BASE COUNT          435 a          279 c          330 g          499 t
ORIGIN      
        1 taatgatgta gtcactgttg atgaaatctc caagacttgg gaaggtgaaa gtcttttaaa
       61 aaatgtgagt tttactttaa agaagggaga taaagtagtc attctcggtc gtaacgattt
      121 agcaaagact ttactgatgg aaatccttgc tggtgaatta tcagctgact ctggttcttg
      181 tacttggggc attacgactt ccattaatta ctttcctact gataactcga aatatttcaa
      241 aggttctgag gaaactttag tcgattggct tcgcccttat tcaaaagaaa aagatgagac
      301 ttttttacgg ggcttcttgg gaaaaatgct tttcagcggc agtgacgcta tgaagaaacc
      361 caatgttctt tccggaggag aaaaagttcg ctgcatgttt gcgcgaatga tgctgtctgg
      421 ggcaaatatt ttaattcttg atggtccaac gaatcatttg gatcttgaaa gcatttcggc
      481 agtgaatgaa gggttaagtc gctttaaggg gacagtgatt tttacctctc atgatcatga
      541 attgatccaa accgttgcca atcgtattat tgaaattgat caagccgtgg tttacgacaa
      601 tcatatctct tatgaggaat atttatcttt aaaaagtcag ggtaaagcat aacattgctg
      661 gggtgggata ggtccaataa agtaaaaaag ctaagacgtc tttcttattt aagagtagaa
      721 ttctcatatg gaagacctcc cagtagaatc gtttatattt tcatttagtt ttggctcctt
      781 gattgctggt ttcatttttg gcgtcatcgg attttggctt ctaggtcatg ggcgtaagaa
      841 agccaataat cggaatgtcg ttattggtct tcttttaatg atgtatccct attttgtaag
      901 gggcgcctgg cttaactggg gaattggctt tgctctctgt ggatatgctt actacacctg
      961 ggattgaaga cttcttactt atttaatttt ttgggatcaa tattcgcagg gattgcagtg
     1021 cctctccacc gaagggtctg ataggtccca ttttttacct tttcagattt cacgtcgaca
     1081 tctacgatac cattgtagtt tccttgagca gcaaaaaagg caagtcgcaa gacagtctca
     1141 tcgcggtctt tacattccac aacctgaaca ggatcagcta gacgctttac gaggcgtgtt
     1201 tcctttccct gatcattaaa aaacacggcg atttcctttg ccgcttccat ggtctgatta
     1261 tatgtctcta tttcggggct cactttaaaa tcaaagcagg ggccacagaa agttccggta
     1321 gaaaattcta caggaatgtg aggtaaaaat gaaaaagtat cctcatcgat aaactgagca
     1381 catttcttac agattgcatt tttacaaatg ccacactcaa gagtggcctt cggtttctgg
     1441 cagattgaac aaaatggagc tttcatacat gaaaactacc acaaggcttt agtgttaggg
     1501 aggtttttat ataaaatccc atcgattcta aaaatttaga ttt
//
LOCUS       DATBTE010000228         4534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2353200, whole genome shotgun sequence.
ACCESSION   DATBTE010000228 DATBTE010000000
VERSION     DATBTE010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2353200"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..753
                     /locus_tag="VIG33_08360"
     CDS             223..753
                     /locus_tag="VIG33_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270888.1"
                     /translation="MEMEAENKDVNKSSHTVFFEGSEGVRSKTSTRIFYDAQVSVIKK
                     QLGNLEKIRADLGLSQRKICQLLMVDPSAWTRWNREGEEAPPHIWRSLQWYMTLQEKI
                     PGLTPQYFIGKDPQILHEKALAKIDEEVQHLKSSFQEQQGRLLKDLRFYRVFNYVLIG
                     ITLLITALFVFLRRIS"
     gene            755..1927
                     /locus_tag="VIG33_08365"
     CDS             755..1927
                     /locus_tag="VIG33_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8270889.1"
                     /translation="MIWPYIIISYFSLFVFGLTDNLRGPLFPEILNTFEISDSFGSWM
                     FAISSISGFAASYVARHLLRRFDRKSVLQGGCLSMIFGLLGLAWSPQFNIFLGFSFLL
                     GLSLGIIGLIPNVLVSLGAPPHLKQRLLSGLHAMYGLASFCAPLVVAQVGAITGSWRP
                     VFLVAIAGPLSLLLYSLHSTHHNLHEKPERKPEQPSLRKSHPLIPQLFIACMLSFDVA
                     GEIMISSRLALFMRREWNFDLQQSSLYVTYFFIAMLVGRALFAIVHFRSSIRTLLSTS
                     IVLTLAAGFAGLYIHPLFLALTGFTIAPFYPLSIAFFSSEFPEDLDSAVSYMMAMDSL
                     MLVFMHLVVGRMTDLWGIHQAILIGMGFFACSFIFLNSYNHVFRKKNSPTTGIRPS"
     gene            2083..3198
                     /locus_tag="VIG33_08370"
     CDS             2083..3198
                     /locus_tag="VIG33_08370"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEY8270890.1"
                     /translation="MGIFELISKHGDHEQVVFCNDPSVGLKAFIAIHNTALGPALGGT
                     RMWQYKSEEEALVDVLRLSKGMTYKAAAAGLNLGGGKAVIMGDPKKDKSEGLFRAFGQ
                     FVNSLNGRYITAEDVGTNVTDMEHIFMETPWVTGIPKGFGGSGDPSPYTAHGVLMGIK
                     AAAKEKFGTDSLMGIKVAVQGLGNVGSNLVKYLIEEGAKVIVADIDQTRVKNMHDQFG
                     CEVSNPEQILTVECDIFAPCALGAVVNDTTITKFKAKAIAGGANNVLAEARHGDQLRE
                     LGILYTPDYVINAGGLMNVFVELEGYSPDRAFDKTRKVYENCMKVFEIAKRDNIGTHI
                     AADRLAEERMKTIGQLKQRHPGKSSRAFSTLREVFNR"
     gene            3354..4166
                     /locus_tag="VIG33_08375"
     CDS             3354..4166
                     /locus_tag="VIG33_08375"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8270891.1"
                     /translation="MSTKLSKDDVKNPDQVLKTLTQGFQWTTNHSRAVVLGVLAFVVI
                     GGGMAIFSGLAERKETQAQEAYFKFEKNYLDKKRDFEEADRNATRPPAKDPKEKPVAK
                     SKATGDLEKDYGPEVAGFKQVMETHPSSKAAQMAALNLSEIYLGYKKLDEASQSLEKV
                     AAQAGSNNLISSIVLTQYGNVLAEKNDCKTAIDQWQKVLSRSEVSFLHDTLRLKSAFC
                     YEKMNDFAKAEELYKKVSQNSQDPKNPQAGEVGLGKDSEKYLRLLQLKKNAG"
     gene            4271..>4534
                     /locus_tag="VIG33_08380"
     CDS             4271..>4534
                     /locus_tag="VIG33_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270892.1"
                     /translation="MLNRTSEKREFQIREVWARQGPEKDNLAFRKINRMTPLILGNLV
                     IEANAIDGIVAYDRETGSPLWRLNVLNGVEAGAAVIKDRLFFGA"
BASE COUNT         1213 a          921 c         1008 g         1392 t
ORIGIN      
        1 actaaagaaa ataactaaaa gctttagttt aatttgaaaa agtctatatc agcctatgca
       61 tttaagaaat tcccatacat atcaagtatt tagtttgacc ctgctggaag attacttcag
      121 tatgtattgt attacgcaac gactatttat gtgaaacaca ttaataatat tgttatttac
      181 aacattctac atttgcaatg ctatgcatta aagtgaggtt gaatggaaat ggaagctgaa
      241 aataaagacg ttaataaaag ctctcacacg gtgttttttg agggttctga gggggttcga
      301 tctaagactt caacccgaat cttttatgac gctcaggttt cagtcattaa aaagcagctc
      361 ggaaatctcg agaaaattcg tgcagattta ggcctttcac agcgaaaaat ctgccaactt
      421 ttaatggtgg atccatcggc ttggacgcgt tggaatcgcg aaggggaaga agctcctcca
      481 cacatttggc ggtctttgca gtggtatatg actctgcaag aaaaaatccc gggcttaacc
      541 ccacaatatt ttattggaaa agaccctcag attttgcatg aaaaagctct tgcgaaaatt
      601 gatgaagaag ttcagcacct caaaagctct tttcaggagc agcagggaag attactcaaa
      661 gatttgcgat tttatcgtgt ctttaattat gtactgattg gaataacact tttaataacg
      721 gcgctttttg tatttttaag gcggatcagt tgaaatgatt tggccttata ttattatttc
      781 ctacttcagc ctttttgttt tcggtcttac cgataatctt cggggccccc tttttccgga
      841 aattttaaat acttttgaaa tctctgattc ttttgggtct tggatgtttg cgatcagctc
      901 tatttctgga tttgccgcaa gctatgtggc cagacattta ttgagacgct tcgatcgcaa
      961 gtccgtacta caaggcgggt gtctttctat gatttttggc cttctagggc ttgcctggtc
     1021 cccgcaattt aatattttct tgggctttag ctttctttta gggctaagtc tggggatcat
     1081 cggtttaatt cccaatgtct tggtgtctct gggagcgcct cctcatttga aacaaagact
     1141 gctttcagga cttcatgcaa tgtatggtct ggcgagtttt tgcgcccctt tggtggtggc
     1201 ccaggtcggg gctataacgg ggagttggcg tccggtattt ctggtcgcca ttgcgggacc
     1261 tttgagtttg cttctttatt ccctgcactc cacccatcac aatcttcatg aaaaaccgga
     1321 acgaaagccg gagcaaccct cccttagaaa aagtcatccc ttgattcccc agcttttcat
     1381 tgcctgcatg ttgagctttg atgtggccgg tgaaatcatg atctcctctc gcctggcttt
     1441 gtttatgcgt cgggaatgga atttcgatct tcaacagtcg agcctctatg tcacttactt
     1501 ttttattgcg atgttggtcg gtcgggcgct ttttgcgatc gtacattttc gcagctctat
     1561 tcggaccttg ctatcaactt cgattgtgct cacgctcgca gcaggctttg cgggattgta
     1621 tattcatcct ttgtttttag ctctgacggg ttttaccatt gcaccgtttt atccactctc
     1681 gatcgccttt ttttccagtg aatttccgga ggatttggat agtgcggttt cttatatgat
     1741 ggcaatggat tcgctgatgt tggtgtttat gcatttagtg gtgggaagga tgacagatct
     1801 gtggggaatt catcaggcca tactgattgg catggggttc tttgcgtgtt catttatttt
     1861 tttaaatagt tacaaccacg tttttcgaaa aaagaattct cccacaacag ggattcgccc
     1921 ctcctaaaac tttgaccatt tatcttcttt tattatttta ttaatcaaaa aaacctccga
     1981 cacagcaaaa ctgttgcgga gcataactgc tggtattttt acctaaagtt acccatcatc
     2041 tccagattcg taatacagga tcagatcaca aaggagaatc ttttgggtat atttgaattg
     2101 atttctaagc atggcgacca cgagcaagtt gtattctgta atgatccaag cgtgggttta
     2161 aaagccttta tcgccatcca taatactgca ctgggtcctg cccttggcgg aaccagaatg
     2221 tggcaataca aaagcgaaga agaagctttg gtggatgtcc ttcgcctctc caaaggaatg
     2281 acttacaaag ccgctgctgc tggcctcaat ttgggcggag gtaaagcggt tattatggga
     2341 gatcctaaaa aagataaatc tgaaggtctt tttcgcgcct tcggtcaatt cgtaaactct
     2401 ttgaatggtc gttacatcac tgctgaagac gttggcacca atgtcactga tatggaacac
     2461 attttcatgg aaactccttg ggtcaccggg attcccaaag gtttcggtgg gtctggcgat
     2521 ccgtctcctt atacggccca tggcgttttg atgggaatca aggcggcggc taaagaaaaa
     2581 tttggtactg actctttaat gggcattaaa gtcgcagttc aaggtcttgg caatgtcggt
     2641 tcgaacttag taaaatatct tatcgaagag ggcgctaaag tcattgtcgc tgatatcgat
     2701 caaactcgcg taaagaatat gcacgatcaa ttcggttgtg aggtctctaa tcccgaacaa
     2761 attcttacag ttgaatgtga tatttttgct ccttgcgcct tgggagccgt ggttaatgat
     2821 actacgatca ccaaattcaa agctaaagcc attgccggtg gcgccaacaa tgttttggcg
     2881 gaagcacgtc atggcgatca acttcgtgag ttggggattc tctacactcc agattatgtg
     2941 attaatgccg gtggcctgat gaacgtattt gtggagcttg aggggtactc tccggaccgc
     3001 gcttttgata aaactcgcaa agtttatgaa aattgcatga aggtgtttga gattgctaaa
     3061 cgagacaata ttggaactca tattgctgca gaccgtttag ccgaagagcg catgaaaacc
     3121 attggtcagc ttaaacaaag acatccgggc aaatcaagtc gtgcattctc gactctgcgc
     3181 gaagtcttta atcgctaaga tttagtcctt tttaagaaat tttgtgagaa cggccctttg
     3241 tctttatgcg gcagttctcc ttttcaaggg gcttttaaaa gcacccttga caacaaatcc
     3301 tcgtcatcat tcaataatct ccttaatcta actattttaa agaggatatc ggtttgagca
     3361 caaagctttc aaaagatgac gtaaaaaacc cagatcaagt tctcaaaacc ctcactcagg
     3421 gctttcaatg gaccacgaac cattctcgcg cagtcgtcct aggtgtcctt gcctttgtcg
     3481 ttattggcgg gggcatggcg atttttagtg gcctggcaga aagaaaagaa actcaagcgc
     3541 aagaagctta tttcaaattt gaaaaaaact accttgataa aaagcgtgat tttgaagaag
     3601 ccgacagaaa tgcaactcgt ccgccagcaa aagatccaaa ggaaaagcca gtggccaagt
     3661 caaaagcgac gggggatctt gaaaaagatt acggccctga agtggctggt tttaagcaag
     3721 ttatggaaac ccatccttct tcaaaggcag cgcaaatggc agctttaaat ttgagtgaaa
     3781 tttacctggg ttacaagaag ctggatgagg cttctcaatc tcttgaaaaa gttgccgctc
     3841 aagctggcag taataattta atttcctcta ttgtactgac tcaatatggg aatgttttag
     3901 ctgaaaaaaa cgattgcaag acagctattg atcaatggca aaaagtttta tctcgttccg
     3961 aagtgagttt cctccatgac acccttcgtt tgaagtctgc cttctgttat gagaaaatga
     4021 atgatttcgc aaaggctgaa gaactttata aaaaggtcag ccaaaactcc caagatccta
     4081 aaaatcctca agccggtgaa gtaggtcttg gtaaagattc ggaaaaatat cttcgtttgt
     4141 tacaacttaa aaagaacgct gggtaaacca gcttcttgtt gaataaggat ttactttgag
     4201 aagcttctct ttttttgttt ttgggtcatt cattatagga cttctatcag ggtgctcaag
     4261 ttttgagact ttgctaaacc gtacttctga aaaaagagag tttcaaattc gtgaagtctg
     4321 ggcccgtcaa ggtcccgaaa aagacaatct ggccttccgc aaaatcaatc gtatgacgcc
     4381 tttaattcta ggcaatttag tcattgaagc caatgctatc gacggaattg tggcttacga
     4441 ccgtgaaacg gggagtccgc tttggcgttt gaatgtttta aatggggttg aggcgggtgc
     4501 ggccgttatt aaggatcgct tgttcttcgg cgct
//
LOCUS       DATBTE010000229         2774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_99029, whole genome shotgun sequence.
ACCESSION   DATBTE010000229 DATBTE010000000
VERSION     DATBTE010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99029"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VIG33_08385"
     CDS             <1..400
                     /locus_tag="VIG33_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8270893.1"
                     /translation="RQLKPNEAEVFVENNAVKVRLKGVQFGSNQTVLNKKSTALLEKV
                     DQALGTVGASSIVVEGHTDSVGSAEANKEISEKRAQAVQNYLVQKGQIASDQVKAVGL
                     GEEGAISDNKTSRGRAENRRIDLVIEPRVE"
     gene            596..802
                     /locus_tag="VIG33_08390"
     CDS             596..802
                     /locus_tag="VIG33_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270894.1"
                     /translation="MKRQRNERAENKSENRFSREESRKHFDRRESERHGDEDYQDFSN
                     RDPFFDDEGIRNPKAYRHSENRRR"
     gene            complement(823..2310)
                     /locus_tag="VIG33_08395"
     CDS             complement(823..2310)
                     /locus_tag="VIG33_08395"
                     /inference="COORDINATES: protein motif:HMM:NF015387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HEY8270895.1"
                     /translation="MKNTSRLLLSIIASLLCFFSFQNCGPAKLNGEASQNGSLDSASQ
                     STLPDLVVSDFSLNPKNPQEGQSVIFSVTVINQGQASTPSGLSTDVAFMVDGIEVAKA
                     NLVVPPLNPGEAVAFTSNQGSVGTNGWISTAGSHSLKVLIKGNNLEETSTDNNLFEAA
                     FTVQASATPCQFAGTSINSGQDVVAYQSSSVSFGSTCNSEARSCLNGTLSGTYQFASC
                     SVGTAANCMFNGATIANGQNVTAYQASNVPFGSICSSQTRTCTNGTLSGTYHFNSCSV
                     LSPLNCQFNGATIANGQNVTAYQASNVPFGSICGSQTRTCTNGTLSGTYQFNSCSVLA
                     PTSTPYTGTPIPVPGSFEAEAYDQGGEGRAYHDNVKENSGGVFRFEDVDITDSTDTLG
                     GGYVVNNLENGEWLVYTINATSSGSYTVGLRTSQSYLPNANFHLEIDNVPVTGSVLVS
                     NTGSWDTFQWTYAPTITMTAGTHQLKIVVDTQYFNLNSIYITKVP"
     gene            complement(2444..>2774)
                     /locus_tag="VIG33_08400"
     CDS             complement(2444..>2774)
                     /locus_tag="VIG33_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8270896.1"
                     /translation="LSMETENSLLDQTTDVIQEALLQFIKDHFKYGRSAKTAYQWSGI
                     MGFTPDEQMLIGSLPENSAIHLMAGCSGHGMGLSFHAAKVLVRSLFGHPIPAHLALQR
                     FSFEHLP"
BASE COUNT          793 a          571 c          691 g          719 t
ORIGIN      
        1 tcgacaatta aaacctaatg aagctgaggt ttttgtagaa aataatgctg ttaaggttcg
       61 acttaaagga gttcagttcg gttctaatca aaccgttctc aataaaaaga gcacagctct
      121 tttggaaaaa gtggatcagg ccttaggcac tgtgggcgca tcatcaattg tagtcgaagg
      181 ccatacggat tcggtgggat ccgcggaggc aaacaaagag atttctgaaa agcgggctca
      241 ggcggtgcag aattatttgg ttcaaaaagg tcaaatcgct tcagatcaag tcaaagcggt
      301 ggggttgggt gaagagggtg cgatttctga caataagact tctagaggac gcgcggaaaa
      361 tcgtcgcatc gacttggtaa ttgagccgag ggttgaataa acgcacacct ttaaaattta
      421 cactgttgaa ggtcactgga aactgtgacc ttttttattt ttgatgagaa tatccttatt
      481 gtttcaataa atcataactg agatatcttt gcattctttt ttctcttcgt cgaacttaaa
      541 agccttgaaa tcaattcaag caaaatgacc atccagactt ccgtcaggag aaattatgaa
      601 aagacaaaga aatgaaagag cggaaaataa atctgaaaat cgtttttcac gcgaagaaag
      661 tcgtaaacac tttgatcgcc gggagagtga gcgtcacggt gatgaggatt atcaagactt
      721 ctcaaatcgc gacccattct ttgacgacga aggaatccgt aaccccaaag cctacagaca
      781 ctctgagaac cgtcggcgct gaaaggaaat aatttttgca aactagggga ccttcgttat
      841 ataaatcgaa ttcaggttga aatactgcgt atccacaaca atcttcaatt gatgagttcc
      901 cgctgtcatt gttatggttg gggcataggt ccactggaat gtgtcccaac tccccgtatt
      961 tgacaccagg acggaaccgg tgactggaac attatcaatc tctaaatgga agtttgcatt
     1021 cggaagataa ctctggctag tccgcagacc gacagtatag cttccggagg aagttgcatt
     1081 gatcgtatat accagccact ctccattctc gagattattg acgacataac ctcctcccaa
     1141 cgtatctgtg gaatcagtga tgtcgacatc ttcaaaacga aatactcccc cgctattttc
     1201 cttcacatta tcatgataag cacggccttc tccaccctgg tcataggcct ctgcttcgaa
     1261 agagccggga acgggaatcg gtgtccctgt ataaggcgtt gaggtaggag ccaggaccga
     1321 gcaggaatta aattgataag tgccgctcag agtgccgttt gtacatgtgc gtgtttggga
     1381 accacagatg gaaccgaagg ggacattgga ggcttggtaa gctgtaacat tttgtccatt
     1441 ggcaatagtt gcgccgttga actgacagtt caacggggat aggaccgagc aggaattaaa
     1501 gtgataagtg ccgctcaggg ttccgtttgt gcaggtgcgt gtttgggaac tacaaatcga
     1561 accgaagggg acattggagg cttggtaagc tgtaacgttt tgtccattgg caatagttgc
     1621 tccattaaac atgcaattgg ctgcggtccc cactgagcag gatgcaaatt gataagtgcc
     1681 actcagagtt ccatttaagc aagatcgggc ttcagaatta caggttgagc cgaaggatac
     1741 gctggagctt tggtaagcta caacatcttg accactattg atggaggttc ccgcaaattg
     1801 gcaaggggtc gctgaggctt gaaccgtaaa ggcagcttca aagagattgt tgtcagtgga
     1861 agtctcttcg agattattcc cttttatcag gacttttaat gaatgggagc ccgcagttga
     1921 aatccagcca ttggttccaa cagatccttg gttgctggtg aaagcgacag cctcacctgg
     1981 atttaaaggg gggactacaa ggttggcctt tgcgacttca atgccgtcaa ccatgaaagc
     2041 gacatctgtg gagaggccac tgggcgtgga cgcttgtccc tgattgatga cggtcacact
     2101 aaagataacg gactggcctt cctgtggatt cttcgggttc agggaaaagt cagacacaac
     2161 gagatccggc aaggttgact gagaggctga gtccaagctg ccattctggg aggcctctcc
     2221 attaagtttg gccggaccac agttttgaaa tgaaaagaag cagagtaagc tagcaattat
     2281 agaaagcaga agacgcgaag tgtttttcat atattcccca attcattcaa tcttcgcata
     2341 aatagggagg attaagaatt tcattttgtt tatatctcaa atatagaaaa taggtgctct
     2401 ataatgtctg gcagggaaat gtccccattc gggacagggg cttctacggc aggtgttcaa
     2461 aggaaaacct ttgaagagcc aaatgggcgg ggatagggtg accaaataaa cttcgaacca
     2521 agaccttggc cgcatgaaaa ctcaatccca tcccgtgccc tgagcatcct gccatcaaat
     2581 gaattgcgga gttttcaggt aaagagccaa taagcatttg ttcatccggg gtgaagccca
     2641 tgatgccaga ccattgatag gcggttttgg ctgatcttcc atacttaaag tgatctttga
     2701 taaattgaag taaggcctcc tgaatgacgt cagttgtttg atctaagagg ctgttttccg
     2761 tttccattga gaga
//
LOCUS       DATBTE010000230         7275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_30473, whole genome shotgun sequence.
ACCESSION   DATBTE010000230 DATBTE010000000
VERSION     DATBTE010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30473"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VIG33_08405"
     CDS             <1..438
                     /locus_tag="VIG33_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8270897.1"
                     /translation="AEDFDAVINTNLRGTFLMTKALSKIMMKARKGSIVNIVSVVGQT
                     GNPGQANYAASKAGTEAFSKSVALELSSRNIRVNCVAPGFIATEMTHVISPEAKTKFL
                     EKIPLNAIGEGQDVANAVKFLLSEESKYITGHTINVNGGLYMS"
     gene            474..710
                     /gene="acpP"
                     /locus_tag="VIG33_08410"
     CDS             474..710
                     /gene="acpP"
                     /locus_tag="VIG33_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY8270898.1"
                     /translation="MEIHPKVKDIIVEQLGVDPEKVKAEASFIDDLGADSLDIVELVM
                     AMEEEFDLEIPDEDAEKLKTVQDVASYLQKKGKA"
     gene            717..1982
                     /gene="fabF"
                     /locus_tag="VIG33_08415"
     CDS             717..1982
                     /gene="fabF"
                     /locus_tag="VIG33_08415"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY8270899.1"
                     /translation="MNTRFERPAKAQKRVVVTGVGAVTPLGLSIQESWENAKAGKSGI
                     AKITRFDTTGHDVSFAGEVKGFNPDLYVEKKEQKKMDTFIHYSLASTKMALEMAKLQI
                     TEELGPRAGCIIGVGIGGLPAIEEQCAKIMSRGPSRITPFFIPMVITNLAAGQVSIAH
                     GLKGPNYSVTSACASGVHSIGEAVNYIRNGWMDVMVAGGAESTICPLAIGGFAAMRAL
                     STRNDAPEKASRPWDKERDGFVLAEGAAILILESLEHAERRGANILCEITGYGVSGDA
                     YHMTSPAPEGAGGYAAMAMAIGDAGLKPSDIQYVNAHGTSTPMGDTLESAAIKKLMGA
                     DSRSIWVSSTKSMTGHALGAAGAIESAFCVMALKDQVAPPTINLENASEGCDLDYLPN
                     EARQGKLNHVMNNSFGFGGTNACLVISKL"
     gene            1979..2440
                     /locus_tag="VIG33_08420"
     CDS             1979..2440
                     /locus_tag="VIG33_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEY8270900.1"
                     /translation="MIKKIFIASDHAGFDLKQEFIKNNPQLPWEDLGPYSAQSVDYPD
                     YAKKLCEHLIEKTGSASSTEDAFKVEACGVLICGSGQGMAIRANKFPQIRAALCWNED
                     VSCLSRQHNNANVLCLGARAVTGDEAQKILNAFLNSTFEGGRHSQRVAKLC"
     gene            2502..3737
                     /gene="glyA"
                     /locus_tag="VIG33_08425"
     CDS             2502..3737
                     /gene="glyA"
                     /locus_tag="VIG33_08425"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY8270901.1"
                     /translation="MSFLINTDPEIAKLIEQEQDRQEYGLEMIASENYTSQAVMEAQG
                     SCLTNKYAEGYPNKRYYGGCHYVDLIESQAIDRAKKLFGVEAANVQPHSGSQANMAVY
                     LACMKAGDTVLGMDLSHGGHLTHGSPVNFSGFLFNATSYKLDTNTERINYDTILKTAR
                     EVKPKLIIAGYSAYPRVLDFAKFREIANEVGAQLMVDMAHFAGLVAGGAHPSPVGYAD
                     YITTTTHKTLRGPRGGMILCSEDKIKALNSKIFPGIQGGPLEHVIAGKAVAFGEALKP
                     EFKQYIENVVVNAKTLAEELLSQGFKLVTGGTDNHLILMDLGDREITGKLAETSLDEA
                     GITVNKNTVPNEKRSPFVTSGIRIGTPALTTRGMKTQDMKQIGKWIAQVLNNPEDTAL
                     KGSIKKDITDMCKKFPIYK"
     gene            3915..4472
                     /locus_tag="VIG33_08430"
     CDS             3915..4472
                     /locus_tag="VIG33_08430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY8270902.1"
                     /translation="MLMVFGSCSSFQTPISRAITQEPLRLPELQPVASAVIKKPEFID
                     NTDSFDWPVNEARMTRGFLPQKRRPHLGIDLAAKRGTPILAAQSGTIIYTGREFRGYG
                     KMILIESRKGWATLYAHMDKILVQEGQKISQGQLLGAMGRTGRATGVHLHFEIRKDRG
                     PIDPLPLLPNGHKVATYLKEHSSQN"
     gene            4559..5707
                     /locus_tag="VIG33_08435"
     CDS             4559..5707
                     /locus_tag="VIG33_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8270903.1"
                     /translation="MSLRTLKKGEVLYTEGEKIHNIYLIQSGAVSLCLTRNKKTTDLF
                     QIGSAQILGEQVLLGQNTHTTSAIATTETKVVEITAEAFKTQFEAAQQMLKIIIKSLA
                     DRLKIATADVKSSRMEKDATPCPEDQVAKVFGVVFHTANHKGEKKDNRVIIEWGLFRQ
                     YSQRVFGESLKRLEQCVNLLVKLKLAMYEMGKNPEDPEGPDHIQKVHFFDLEAIENFF
                     EFYQYYYFKANKPEMLKIEDTSASILSGLIAFAETQPMDRFGVVTLEFPKLIEYFKNE
                     FSVNFTPDTLTRLEKKGLFTKRRTNSNNEVQLQFDFKEFSGFQKNWRLLREVDKWNEK
                     GFVDPHEAEKKPAKKASAGGVKCPACSAESQENQKFCGECGHKLLAAA"
     gene            complement(5781..6707)
                     /locus_tag="VIG33_08440"
     CDS             complement(5781..6707)
                     /locus_tag="VIG33_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270904.1"
                     /translation="MKRLFVMTVLTTLVTAAQAEEAVQSTVAASTTTTASAPATVASA
                     TSGTTSGSLLEAETAAKSKWSFNALAQAEIASSEMHYSNSTDKTVDFTNAVGLGYKFN
                     KENSIGFKQYFDVKHDGQKKTNKADLSYPVLTYGHTFQGVAKSDPVSALFWYYVPVTA
                     TDYKVQNNGLLRMDAEVNWTLSPKWSLSYYLNPRQSLIPTETTLDDNPVFAKTTLIHF
                     GTLYYNVNDTISYYLNVGFRHDWKTASLDLVKEQYLTNLGASFSFFGGKLNLNPEIDY
                     AVVLPRSGQSLEVASTYAEANLSYLLTTAVVF"
     gene            complement(6896..>7275)
                     /locus_tag="VIG33_08445"
     CDS             complement(6896..>7275)
                     /locus_tag="VIG33_08445"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEY8270905.1"
                     /translation="IGKSLGPKVFSREKSLFLNPKYLKQTQEFYRDHGGKTVFLARFM
                     PIFRTFAPFVAGVGRMHFGKFFAFSVVGSVVWMSVFLGAGYYFGNLPSVKSNFHIVIF
                     TVIGVSFLPMLMTALKHRFSSHL"
BASE COUNT         2130 a         1583 c         1588 g         1974 t
ORIGIN      
        1 gcggaagact ttgatgcggt tataaatacg aatctgcgcg gcacctttct tatgaccaaa
       61 gccctgagta aaatcatgat gaaggctcgc aaaggatcca tcgtaaatat tgtcagcgta
      121 gtgggacaaa ctggaaaccc tgggcaagcg aattacgcgg cctctaaagc aggaacagag
      181 gcttttagca aatctgtcgc cttagaactc tcctcgcgca acattcgtgt aaactgcgtt
      241 gctcccggat ttattgcgac cgaaatgacc catgttattt ctccagaggc aaaaacaaag
      301 tttcttgaaa aaattcctct caatgcaatt ggggagggtc aagatgtcgc caatgctgtt
      361 aaatttttac taagtgaaga atcaaaatat attacaggcc ataccattaa tgttaatggt
      421 ggcttgtata tgagttaatt tttaatgaaa ttgaatttta ccaggaggat ataatggaaa
      481 ttcacccaaa agttaaagac atcatcgttg aacaacttgg cgtagaccct gaaaaagtga
      541 aagcggaagc ttcatttatc gatgatttgg gtgcagacag tcttgatatc gttgagcttg
      601 taatggcaat ggaagaggaa ttcgacctgg aaattcccga tgaagatgct gaaaagctga
      661 aaacagttca ggacgtagct tcttatttgc aaaaaaaagg taaagcataa gcttttatga
      721 acactcgatt tgaacgccca gcaaaggctc aaaaacgtgt ggtggtcact ggtgtaggtg
      781 ctgtaacccc tcttggactt tctattcaag aaagctggga gaatgcgaaa gcggggaagt
      841 ccggcattgc taaaatcacc cgtttcgata ccaccggtca cgatgtgagc tttgcaggtg
      901 aagttaaagg ctttaatcca gatctttatg ttgagaaaaa agaacaaaag aaaatggaca
      961 cctttattca ctactcttta gcctctacaa agatggctct tgagatggcc aagcttcaaa
     1021 ttaccgaaga gctgggaccc cgtgctggtt gtattatcgg cgtcggcatt ggtggacttc
     1081 ccgctatcga agagcagtgt gcaaaaataa tgagtcgcgg cccaagccgc attactcctt
     1141 tctttattcc aatggtgatt acgaacttag ccgcagggca agtgagcatc gcccatggac
     1201 ttaaaggtcc taattattct gtgacctcag cttgcgcttc aggagtccac tccatcggtg
     1261 aggctgtgaa ctatatccgc aatggttgga tggacgttat ggttgcggga ggtgcggaat
     1321 ctacgatttg ccctttggca attggtggat ttgcagccat gcgagctctt tcaactcgta
     1381 atgacgcccc tgaaaaagct agtcgccctt gggataaaga gcgtgatggt tttgttctcg
     1441 ctgaaggagc tgctatcctt attcttgaat ctctcgaaca tgctgaaaga cgaggagcaa
     1501 atattctctg tgaaattaca ggttatggcg tttctggcga tgcctaccat atgacctcac
     1561 cagccccaga aggcgctggg ggatatgcgg caatggcaat ggcgattggc gatgctggac
     1621 ttaaaccttc agacattcaa tatgtgaatg cccatggcac aagcactcct atgggagata
     1681 ctcttgagtc cgctgctatc aagaaattga tgggggctga ttctagaagt atctgggttt
     1741 cttctaccaa gagcatgact gggcacgcac taggagccgc cggagctatt gaaagtgctt
     1801 tctgtgtcat ggcattgaag gaccaagtcg cgccacccac aatcaatctg gaaaacgcca
     1861 gtgaaggctg tgatttagac tatcttccta atgaagcccg tcagggtaaa ttaaatcacg
     1921 ttatgaacaa cagttttggt tttggcggca ccaatgcctg tttggtgatt tctaagttat
     1981 gataaaaaaa atcttcatcg cttcagatca cgcagggttt gacttgaaac aagagtttat
     2041 caagaacaat ccccaacttc cctgggaaga tttaggccct tactccgctc aatctgtgga
     2101 ctaccccgac tacgctaaaa aactttgtga acaccttatt gaaaaaactg gttcagcttc
     2161 aagcactgaa gatgctttta aagtggaagc ttgtggagtt ctcatctgtg gatctggcca
     2221 ggggatggca attcgtgcca ataaattccc tcagattcgc gccgctcttt gctggaacga
     2281 agacgtcagc tgcctttctc gtcagcataa taatgccaat gttttgtgtt taggagcccg
     2341 tgctgtcaca ggggatgaag ctcaaaagat cctcaatgct tttctaaatt ccaccttcga
     2401 aggtggacgc cactcgcaaa gagttgcaaa gctctgttag cactctgtag aataggactc
     2461 tatctttagt cttaatttct cacttatctc ggagcgccag tatgtccttt ttaattaata
     2521 ccgatcctga aatcgcaaaa cttatagagc aagagcaaga tcgccaagag tatggccttg
     2581 aaatgattgc ttcagaaaat tatacgtctc aggccgtgat ggaagcccag ggctcttgct
     2641 taacaaataa atatgcggaa ggttatccta acaaacgtta ctatggtggt tgccactatg
     2701 tcgacttgat tgaatcacag gctattgatc gagcgaaaaa acttttcggc gtagaagcgg
     2761 ccaacgtcca gccccactct ggatctcaag ccaatatggc cgtgtaccta gcttgcatga
     2821 aagctgggga tacagttctg gggatggacc tttctcacgg tggacatctt acgcacggtt
     2881 ccccggtgaa ttttagtgga tttcttttca atgccacttc ttacaaatta gatacaaata
     2941 cagagagaat caactatgac acgattctta agactgcgcg cgaagtaaag cccaaactta
     3001 tcattgctgg ctacagcgct tatcctcgag ttctggattt tgcaaaattt agagaaatcg
     3061 ctaatgaggt cggtgcccaa ttgatggtgg atatggccca cttcgcaggt ctggtcgctg
     3121 gtggagctca tccaagtcct gtgggctatg cggattacat cacgaccacc actcacaaaa
     3181 ctttgcgtgg tcctcgaggc ggaatgattc tatgttctga agataaaatc aaagccctca
     3241 attcaaaaat ttttccagga attcaagggg gcccgcttga acacgttatt gccggcaaag
     3301 ccgtggcctt cggtgaggcc ttaaaacctg aattcaaaca gtatattgaa aatgtcgttg
     3361 tgaatgccaa gactttagct gaagaacttc tttctcaagg attcaagtta gtgaccggcg
     3421 gcactgacaa ccatttgatt ttgatggatc taggcgatcg agaaattacc gggaaacttg
     3481 cggaaacatc tctcgatgaa gcgggaatca cggtgaataa aaacaccgtt ccgaatgaaa
     3541 aacgctcacc ttttgtaacc agcggcattc gcattggcac tccggcactg acaacccgtg
     3601 gaatgaagac tcaggacatg aagcaaatcg gaaaatggat tgcgcaggtt ttgaacaacc
     3661 ctgaagacac cgcactcaaa ggaagcatta aaaaagatat taccgatatg tgcaaaaagt
     3721 ttcctatcta taagtgactc cattaaaatc ctggacgatg atccagacta atgacgaaag
     3781 caccctttaa attcaagtat ttatgactct taacgacaca aatacttgaa ttgtttttgg
     3841 cgtctgacag agttttgcgc tatacttggc tcatgttgac acgtgacgtc tttcggcttt
     3901 ttttttcgtt ctttatgctc atggttttcg gatcttgctc aagttttcaa actcctattt
     3961 cccgtgctat tacacaggaa cccctccgcc ttcctgaact tcagcctgtg gcttcagcag
     4021 tgattaaaaa gccggaattt attgataata cggattcatt tgattggcct gtgaatgagg
     4081 ctcgaatgac gcgaggcttt cttccgcaaa aacgtcgccc ccatttggga attgatcttg
     4141 cggcaaaacg tggcacccct attttggcag cccaaagcgg aacaattatt tatacaggtc
     4201 gcgagttccg tggatacgga aaaatgattc ttattgaatc tcgtaaaggc tgggcaactt
     4261 tgtatgctca tatggacaaa attctggttc aagaaggcca gaagatctct caaggacagc
     4321 ttctgggagc catgggcaga acaggacgtg ctacgggcgt tcaccttcac tttgaaattc
     4381 gtaaagaccg aggacctatt gatcccctcc ctcttttacc taatggtcat aaagtcgcga
     4441 cctacctgaa agagcattca agccaaaact agactaagag aaagcgttaa gttcttttaa
     4501 attttatttt ttttccgatg agtattactg aaaatattaa aatttttagg agcttatcat
     4561 gtctctaaga acgcttaaaa aaggtgaagt cctctatact gaaggagaga aaattcacaa
     4621 tatctatctg attcaatccg gtgccgtctc tctttgcctg actcgcaata aaaaaactac
     4681 ggatctattt cagataggtt cagcacaaat tcttggcgaa caagttttgc ttggacaaaa
     4741 cactcacacc acctctgcaa ttgcaacgac cgaaaccaaa gtagttgaga tcaccgctga
     4801 ggccttcaaa acacaatttg aagccgccca acaaatgttg aagatcatta taaaaagcct
     4861 ggcagaccga cttaagattg ccactgcaga tgtaaagtcc agtcgcatgg aaaaggacgc
     4921 cacgccctgc cctgaagatc aggtggcaaa agtttttggc gttgtctttc atactgcaaa
     4981 ccacaaaggg gaaaaaaagg acaaccgggt tattattgag tggggcctct tccgtcagta
     5041 ttcacagcgg gttttcggcg agtctttaaa gcgccttgag cagtgtgtga acctcttggt
     5101 taagttgaaa cttgccatgt acgaaatggg aaaaaatcca gaagatccag agggtcctga
     5161 tcacattcaa aaagttcatt ttttcgatct tgaagctatt gaaaatttct ttgagtttta
     5221 tcaatactat tacttcaaag cgaacaaacc agaaatgctt aaaatcgaag acacctcggc
     5281 aagtattctc agtggcctta tagcctttgc tgaaactcag ccaatggatc gcttcggagt
     5341 agtcactttg gagtttccga agcttattga gtactttaaa aacgaattca gtgtcaattt
     5401 tacaccagat acccttaccc gtcttgagaa gaaagggctc tttacaaaac gaagaacgaa
     5461 ttccaataat gaggttcagt tgcaatttga tttcaaagaa ttcagtggat tccaaaaaaa
     5521 ttggcgcttg cttcgcgaag tcgacaagtg gaatgaaaaa ggttttgtag acccgcacga
     5581 agctgagaaa aaacctgcta aaaaagcttc agccggtgga gtcaaatgcc cagcttgttc
     5641 agccgaaagc caagaaaacc aaaaattctg cggagagtgc ggtcacaaac ttctcgctgc
     5701 ggcctaaacg atgccttctt aaaaaaagga gcccttttaa aggctcctta gcggttttaa
     5761 atgtcttttt aataaactaa ttaaaacaca actgcagtag ttaaaagata gctcaggttt
     5821 gcttccgcat aagtagaagc aacctcgaga ctctgtccac ttctaggaag aactactgca
     5881 taatcaatct caggatttaa gtttaatttg ccgccaaaga aagagaagct agcaccaaga
     5941 ttggttaaat attgctcttt tacaagatcc aaacttgcag ttttccaatc atgtctaaaa
     6001 cccacgttca aatagtaaga aatcgtatca tttacattgt aataaagagt tccgaagtga
     6061 atcagagtgg tctttgcaaa cactggattg tcatccaatg ttgtttccgt aggaatcagc
     6121 gattgacgtg gattcagata ataagaaagg ctccattttg gactcagggt ccaattcact
     6181 tcagcatcca ttcttaaaag accattattt tgaactttgt agtctgttgc agtgaccggt
     6241 acataatacc agaacaaagc agagactgga tcagacttgg cgaccccttg aaaggtgtgc
     6301 ccataagtga gaacagggta agacaaatcc gctttattgg tctttttctg cccatcatgc
     6361 ttcacgtcga aatactgctt aaatccaatg ctattttctt tgttaaattt atatcctaaa
     6421 ccgacagcat ttgtaaaatc tacggtctta tcagtgctgt tactgtagtg catttcactc
     6481 gaagctattt cagcttgagc caaggcattg aaggaccatt tgcttttggc agctgtttca
     6541 gcttcaagca aagaaccaga cgtggtgcca ctggttgctg atgccactgt tgcaggggca
     6601 ctcgcagtcg tcgttgttga ggctgctaca gtgctctgaa cggcttcctc cgcctgtgct
     6661 gcagtcacca atgtcgtaag aacggtcatt acaaaaagtc ttttcattat ctaccccttt
     6721 aaagaactct ggactttctt tgtcgatttg agttttccct ctcacttcga ctccccttgt
     6781 cacagtagtt attatttaaa ccctggtttc aaattgaacc cacccggact ttagtagcaa
     6841 tgacagtgcc aaatcctgaa aatcattgca acaacaaatg ttcgttatat tgatcttaca
     6901 gatgagagct aaagcggtgt ttgagggctg tcataagcat tggcaggaaa gagaccccaa
     6961 tgacagtaaa tattacgata tgaaagttac ttttgacact aggcaagttc ccgaaatagt
     7021 aaccggcccc taaaaacaca ctcatccata caacagaccc cacaacactg aaggcaaaaa
     7081 acttaccaaa gtgcatgcgt ccgactccgg ccacaaaggg agcgaaagtt ctaaaaatgg
     7141 gcataaaacg cgccagaaac actgtttttc cgccatggtc acgataaaat tcttgagtct
     7201 gcttgaggta cttgggattt aaaaacaggg atttttctcg actaaatacc ttgggtccga
     7261 gactcttccc aattt
//
LOCUS       DATBTE010000231         8053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1737575, whole genome shotgun sequence.
ACCESSION   DATBTE010000231 DATBTE010000000
VERSION     DATBTE010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 8053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8053
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737575"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VIG33_08450"
     CDS             <1..949
                     /locus_tag="VIG33_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HEY8270906.1"
                     /translation="KKAFQAVQATELMAPSTKSVLTVGEESLSKSIQRLGEVDFFAVV
                     TRKPCICDFKPVVVEVALARFKTRQLEADSPVQLLRFANRVPLQFDKSGCAITWAIES
                     VNWKAYGLQQPKDSLPIGPYIFAVSVVSPFIKFKNASKETIDASEELVAEIRLALIQA
                     GQKLSRHIKKESKEADLERKLAHIEQFGPILVESLARIVGANEARKKKAEEGLKKLLG
                     RDSQEAIADLEEAEGKLLAQKKRERQKGVGHEDEEDVDVITSEDLVDEALMEGQPQGT
                     QKQSAQGTQKQSSQGTMKASLQGTMKQATKKIATKKGGK"
     gene            949..2112
                     /locus_tag="VIG33_08455"
     CDS             949..2112
                     /locus_tag="VIG33_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI"
                     /protein_id="HEY8270907.1"
                     /translation="MAKLIAIRELKIDIPKEAKILAEKMLKDLENSKRPVLEAVKTSL
                     DNSFYNSKVGYLTPGDKVVRTELNVSSVQKLARVVFVLEILLRNLSIGSVNTKRELYY
                     ISKGLIKGNSRLKPLDFEDQPESDAIVDFIGDMLEVYREELNCFANDRGGQTYSQQLI
                     VTETMTDGEKATVDLSTLGTSPFQPKNKPQSLKLKAKKKIDFCLVVESEGTANTLMTM
                     GFTKRNNCILMGAQGVPSNGVRGWCKLIQDELNVPMYFFGDLDAYTMQNIFRTLKAGS
                     AASLIRNADFSAPNVRFLGVLPEDVKKYDLPHYKVKESDPAEARALKKAKDVLENDPF
                     FLDKKNKELADILRFLIKEKIRCEQQSFFSVDPNDPIKTEKLILEKIKRGSYV"
     gene            complement(2113..3042)
                     /locus_tag="VIG33_08460"
     CDS             complement(2113..3042)
                     /locus_tag="VIG33_08460"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEY8270908.1"
                     /translation="MRNSLLLTLSLTSILTFIACSKTPRGTEGNQTSIFADSHSSKGI
                     IGGSNVSTSDDITKITVQIFTFQVERDNLGRLGIMGVDGCTGSILAEDIILTAAHCTT
                     GNPNHIILYFSSLPPKNMQDFLRSIPTNPMVRRVVGGKVGNNWPRLTAGQAADWGDIA
                     LLKFKGGLPFGYRQAQLLPPNIELKAQQPVTLAGFGITNGEQETQATHLLKVEVNILD
                     PNFSKSEMILDSGNGKGPCHGDSGGPAYVSINGQRYISGTTSRADLKTDPKGLCIGDT
                     VYTKVQPYAGWIKSAIKTLQSPTFKAAMIPQPQ"
     gene            3535..4107
                     /locus_tag="VIG33_08465"
     CDS             3535..4107
                     /locus_tag="VIG33_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270909.1"
                     /translation="MRKIFKSLVVAVLLSACAKGGGGGDSAPPPVAVVPPAANGCVAG
                     QVNTQYGCLPQSSCPANYGLYNNQCVPMTAVNPNGGGCAGGQLYTQYGCLAQSTCPPN
                     SALYNNMCVPAYANNNGYYGNGYGNSGYYNNYYYQNRNAYYGNGGGGYYYPNPYASGG
                     YYGNAGVGAGVGAGFYIGGHVGVPGAYYGY"
     gene            complement(4169..4576)
                     /locus_tag="VIG33_08470"
     CDS             complement(4169..4576)
                     /locus_tag="VIG33_08470"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HEY8270910.1"
                     /translation="MNRMSNIQEEAASVVGSSNTQLTQAISKIIDECGGMDGLVKRFQ
                     DKGLGDVIQSWISSSPNKPLTADQITNVMGPGLIQKIAKQVGMSPENLSQQLSTYLPL
                     LVDKMTPSGAVPHEGWWARSLDKAKEFFGLTKH"
     gene            complement(4650..6710)
                     /locus_tag="VIG33_08475"
     CDS             complement(4650..6710)
                     /locus_tag="VIG33_08475"
                     /inference="COORDINATES: protein motif:HMM:NF021639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEY8270911.1"
                     /translation="MFQRSRDLITKWAEAPHSRRNLDTLLNSPPPSDALLRKRLEWLT
                     DLIQWIRAKGRIKTDLNFQSGQPQALRAKYLLSVLEYNPTWKKNVAMILRSIVRDTSA
                     LDLFVNMGMHNQQGFSAEMIDRLHYKFLPRAPDYKNLASFFSQTFDKEEDYLWIEQMD
                     KKVFQELLNLFSYEDNGELQWNSFIVDAKDAILLISTQVHGAGLSTPIRTRLLEKNFK
                     DLPFFELPQLAKKMVESQDLHQRLVFALELERHIDLCQLAISEVYDHLSEYGVSINIV
                     FLLDRMETQLRRIRNLMELILSHEKNPEAISQFISTLMVENVRFHKLRALFADNLSLI
                     SKKIVERSAETGEHYITRNRSEYVQSLRKAIGGGFITGFTTLIKFLIYHIHTSIFLSG
                     FLASLNYSISFVAIQLFGFTLATKQPAMTAPSLAAKMHKVNDPKELETLVDEIIHLIR
                     SQFIAILGNIYAVIPTMIVLSFAWLLAFHSPLITPDQAHHALQSFSILGGMAPYAAFT
                     GVLLWFSSVIAGWMDNWFAYHRLSSAISQNRRMIFVFGESGAKRIALYFKKNISGFSG
                     NISLAFFLGLIPAIGTFLGLPMDIRHVTLSSGALTSSLVTLGWSSFSSWEFWLAAIGI
                     MVCGFLNVIVAFSMSMFVAIRARRIQLPQRKQIYKALMKRIKKQPFGLFYPQTTTNDG
                     KNRH"
     gene            complement(6691..7305)
                     /locus_tag="VIG33_08480"
     CDS             complement(6691..7305)
                     /locus_tag="VIG33_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY8270912.1"
                     /translation="MTVSIFAFSLAILVAFTLDQNLSLFFKRPELEGIWLKARAVTNV
                     GLSEHYFLIAILLYFFARWIRPNSLRLREWSRNLFFALLTSGIFVHILKFCFGRQRPH
                     KSPDFNPFVFHPFTFHWDFHSFVSGHTQVLFTVATMLSLALPKGKWLFFILAAFFAFT
                     RVIIHDHFLSDVIAGAVVGYTGTLTAICWTELWQSRRANVPKIS"
     gene            7494..>8053
                     /locus_tag="VIG33_08485"
     CDS             7494..>8053
                     /locus_tag="VIG33_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270913.1"
                     /translation="MAPRMVTQLAFINLVFLISFSVQASSPDYYTVEETEAQKFNHSI
                     HSDSTEGFFSLNPMNAMSEVVRDSAAKVLDFSLSLMNWVHHNEELEDPDVPYNRKNHY
                     GTWVRDRRENNCYNTRARVLIRDSQIPVTFNDKGCTVLSGQWYDPYSNQEYTQASDIQ
                     IDHVVPLKNSYVSGGWKWDSSTRCLYA"
BASE COUNT         2361 a         1669 c         1887 g         2136 t
ORIGIN      
        1 caaaaaggcc tttcaagcgg tgcaagcgac ggagttaatg gcgcccagta cgaagtcggt
       61 tttgacagtg ggagaggaat ctctttcaaa atccattcaa cgccttgggg aagtggactt
      121 ctttgcagtt gtcactcgta agccgtgtat ttgtgatttt aaacctgtgg ttgtggaagt
      181 cgccttggcg cgctttaaaa cccgtcaact ggaagctgac tctcctgtgc agttgcttcg
      241 tttcgccaat cgcgtccctt tgcaatttga taaatcaggc tgtgcgatca cttgggccat
      301 cgaatccgta aactggaaag cttatggtct gcaacagcca aaagacagct tacctattgg
      361 gccttatatc tttgcggtct cggttgtttc tccgtttatt aagtttaaaa atgcttcaaa
      421 agaaaccatt gatgcttctg aggaactggt ggctgagatt cgtctggcct tgattcaagc
      481 aggacaaaaa ctttcccgcc acattaaaaa agaatctaaa gaagctgatc tcgaaaggaa
      541 gttggctcac attgagcagt tcggcccgat tttggtggag agtttagctc ggatcgtcgg
      601 ggcgaatgag gcccgcaaga aaaaagcgga agaggggctt aagaaacttc tcggccgtga
      661 ctctcaagag gcgattgccg atttggaaga ggcggaaggt aaacttttag cgcaaaagaa
      721 aagggaacgt cagaaaggcg tcggccacga agatgaagag gatgtcgatg tcattacttc
      781 ggaagatctt gtggacgagg ctctaatgga aggacagccc caaggaacac aaaagcaaag
      841 tgcccagggg acgcaaaaac aaagctccca gggaactatg aaagcaagtc tccagggaac
      901 tatgaaacaa gccactaaaa aaattgctac aaagaagggt ggtaaataat ggcaaagctc
      961 atcgccattc gtgaacttaa aattgatatt cctaaagaag ccaaaatttt ggcggagaaa
     1021 atgcttaaag atctcgagaa ctcgaagcgt cccgtccttg aggccgtgaa gacttccctg
     1081 gataactcgt tttataattc taaagtcggt tatttgactc cgggagataa ggttgttcgc
     1141 actgaattga acgtttcttc agtgcagaaa ttggcccgtg tagtttttgt gcttgaaata
     1201 cttcttagaa atctgtccat tggttccgtg aacaccaaga gggaattgta ctatatcagc
     1261 aaaggtttga ttaagggaaa ttctcgtttg aaacctttgg attttgaaga tcaacctgaa
     1321 tcggatgcga tcgtcgattt tatcggtgat atgcttgaag tttaccgtga agaattaaac
     1381 tgttttgcca acgatcgcgg cggccagact tattctcagc agctcatcgt gacagaaacg
     1441 atgactgacg gtgaaaaggc gaccgtggat ctttccactt taggaacgtc tccgttccag
     1501 ccaaaaaata aaccacaaag tttgaagtta aaagcgaaga agaaaattga tttctgcctg
     1561 gttgtggaat ctgaaggtac cgccaatact ttgatgacaa tgggatttac caagcgtaat
     1621 aactgtattt tgatgggtgc tcaaggggtt ccttcaaacg gagtgcgggg ctggtgtaag
     1681 ctgatccaag acgagctgaa tgtgcccatg tatttcttcg gagatctgga tgcgtacaca
     1741 atgcaaaata tcttccgtac gctgaaagcc ggctctgcgg cctctttgat tcggaatgcg
     1801 gatttctcgg ctcccaatgt aagattcttg ggtgttttac ctgaggacgt gaagaagtat
     1861 gatcttcctc attacaaggt gaaagagtca gatccagcgg aggcccgggc tctaaaaaaa
     1921 gccaaggatg ttttagagaa tgatcccttc ttcctcgata aaaaaaacaa agagcttgcg
     1981 gatattctgc gcttcttaat taaagaaaag atccgttgcg aacagcaaag ctttttctca
     2041 gtggatccta atgatccgat taaaactgag aaactcatcc ttgagaaaat caaacgcggt
     2101 tcttacgttt aattattgtg gctgtggaat catggccgct ttgaaagtcg gcgattgtag
     2161 cgtctttatg gcgctcttga tccaaccggc ataaggttga accttagtgt aaaccgtatc
     2221 ccctatacac aggcctttag gatctgtttt taaatcagcg cggctggttg taccagaaat
     2281 gtagcgctgg ccatttatgg agacataagc ggggccgccg gagtccccgt ggcaggggcc
     2341 ttttccgttt ccggaatcca aaatcatctc actcttacta aagttcggat caagaatgtt
     2401 cacctctact tttaaaagat gtgtcgcttg tgtttcttgt tcgccattgg taatgccaaa
     2461 gcccgcaagg gtgactggct gctgagcctt gagttcaatg tttggcggta gaagttgtgc
     2521 ctgccgatat ccaaaaggga ggccgccttt gaatttcaag agagcaatgt ctccccagtc
     2581 tgcggcttgg cccgcagtga gtctgggcca gttattgcca actttacctc cgacgacccg
     2641 gcgaaccatg gggttggtgg gaatcgaacg caggaagtcc tgcatgttct ttggcggaag
     2701 agaagagaaa taaagaataa tatgattcgg gttgccggtg gtgcaatgag cagcggtaag
     2761 aatgatatcc tccgcaagga tagaacctgt gcatccgtcc actcccataa tgccgagacg
     2821 tcctagatta tcccgctcta cctgaaacgt aaaaatttgt accgtgattt tagtgatgtc
     2881 atcgctggtg gaaacattac tgccgccaat aattcccttg ctagaatggg aatctgcaaa
     2941 gatggaggtt tgattccctt cagttcccct aggtgtcttt gagcaagcaa tgaatgtcaa
     3001 aatacttgtt agcgatagag ttagtaataa tgaatttctc atgagctcct ccttctcctt
     3061 taactgtttc atcaaaattc taaattcttc tcaagacgat agtttggatc agatccataa
     3121 gatattttta tgggaaaaat gacaccagaa ttcctcattc ggaaagctgt gaatcaatca
     3181 aaaaaggaca ggtgggatca ttagaaaaat ttatggtagt gaagtggtgt cataaaaata
     3241 aaaaaaagaa tttggtgaca ttttgacggt gtaaaattga gaggccgccc tgaaagggcc
     3301 tctcataaac ttaaagaaga atgagcttat gattttttca cagacttgca aaattataaa
     3361 tcaccagttc atatgtcgct tccggagctt gcaaagtaac ttttcacggc actccgtcta
     3421 agtttttgtc atggaaaatc tcccgagttc tttttataca ctctgatcac ggcacagagc
     3481 atgcaataga tggcgcagtg gtttagtgca ttaatgagcg aacgtggagg aattatgcgg
     3541 aaaatattta agtctttagt cgtagcggtc ttgctgagcg cttgtgctaa aggtggcggt
     3601 ggcggcgaca gcgctcctcc accagttgct gtggttcctc cggccgcgaa tggttgcgtc
     3661 gctggccaag tgaataccca atatggatgt ttaccgcaga gtagctgccc agcgaattat
     3721 ggtctttata ataatcaatg tgtgcctatg acagctgtca atccgaatgg agggggttgt
     3781 gccggtggtc aactttacac ccagtatggc tgcttagctc agagcacttg tcctcccaat
     3841 tccgctttgt acaacaatat gtgtgttccg gcctatgcca ataataacgg gtattatgga
     3901 aacgggtatg gaaatagtgg gtactataac aactattatt atcaaaacag gaatgcttat
     3961 tacggcaatg gtggtggcgg ttactattat cccaacccat atgcttcggg tggttactac
     4021 ggtaatgcgg gagtgggcgc gggcgttggc gcaggtttct acatcggtgg ccatgtaggt
     4081 gttcctggtg cgtattacgg ctactaacaa atattaataa tcatcacaat tcagcgaaga
     4141 gtctcaggac tcttcgctga atgcttcatc agtgtttggt taaaccaaaa aactcttttg
     4201 ccttatccag gctgcgagcc caccaccctt catgaggaac cgccccagag ggagtcatct
     4261 tatccactaa caacggcaga taggttgata attgttggct cagattttca ggagacatgc
     4321 ccacttgctt tgctattttt tgtataagcc cgggacccat cacattagtg atttgatctg
     4381 cggttaaggg tttattggga gagctgctaa tccatgattg gataacatcc ccaagaccct
     4441 tgtcttgaaa cctttttaca aggccatcca tccccccaca ctcatcgata atttttgaaa
     4501 tcgcttgagt gagctgagta tttgaagagc caacgacact ggccgcttct tcttggatgt
     4561 tactcatcct gttcataaac ccctccttga attgcctaaa tatctaacaa agaaagttta
     4621 aaagcagaaa atcgcaggat gtagaaaaac taatgacggt tttttccatc gtttgtcgtg
     4681 gtttgcggat agaataaacc aaaaggctgt tttttgatac gtttcatgag ggctttataa
     4741 atttgttttc tttggggcaa ctgaattctt cgcgcgcgga tcgccacaaa catcgacatg
     4801 ctaaatgcca cgatgacgtt taagaatccg cagaccataa tgcctatcgc cgcaagccaa
     4861 aactcccagg aagagaagga actccatcca agagtgacca gcgaagaggt taaggccccc
     4921 gaagacagag tgacgtgacg aatatccatc ggcaggccta aaaaagtgcc aatggcggga
     4981 atcagtccta agaagaaggc caaagaaata tttcctgaaa aacccgagat atttttttta
     5041 aagtagagag cgatcctctt ggctcccgat tctccaaaga cgaagatcat gcggcgattc
     5101 tgagaaatgg cggagctgag acgatgataa gcaaaccaat tgtccatcca ccccgcaatc
     5161 acgcttgaaa accacagaag aactccggta aaggcggcgt agggagccat acctcctaag
     5221 atggaaaaag attgcaaagc atggtgcgct tgatcaggag ttataagagg actgtgaaag
     5281 gccaaaagcc aagcaaaaga aaggacgatc atggtgggaa tgacagcata aatatttccc
     5341 aggatcgcta taaattgcga acgtattaaa tggatgatct catcgaccaa cgtttccagt
     5401 tcttttggat cgttcacctt gtgcattttt gccgcaagcg aaggggccgt catcgcgggt
     5461 tgcttggttg caagagtgaa accgaagagt tgaatggcca cgaaactaat ggagtaattg
     5521 agagaagcca agaaaccact gagaaaaatc gaagtgtgaa tgtgataaat aagaaactta
     5581 ataagagtgg taaatcccgt aataaagcca ccgccaatgg cttttcggag actttgaaca
     5641 tactcggagc gatttcgtgt aatataatgt tccccagtct cggcactgcg ttcaacgatt
     5701 tttttagata ttaaagataa gttgtccgca aagagcgctc ttaatttatg gaagcggaca
     5761 ttctccacca tcagagtgga tataaattgt gaaatggctt caggattttt ttcatggcta
     5821 agaatcaatt ccattaaatt acgaatacga cgaagttgag tttccatccg atcgagcaag
     5881 aaaacgatat tgatactcac accatactca ctcagatgat cataaacttc agaaatcgcc
     5941 aattgacaga ggtcaatatg cctttcaagt tccagagcaa agaccaaacg ctgatggagg
     6001 tcttggctct ccaccatttt ttttgccaat tgcggtaatt caaaaaaagg cagatcttta
     6061 aagttttttt ctagcaggcg ggtgcgaatc ggcgtcgaaa gacctgcgcc atgaacctga
     6121 gtgctgatca gcaaaatggc gtctttggca tccacaataa agctattcca ctgtaattct
     6181 ccattatctt cataggagaa aagattcaaa agctcctgaa agactttttt atccatttgc
     6241 tcgatccaca aataatcttc ttctttatca aaagtctggc tgaaaaaaga agccagattt
     6301 ttatagtcgg gtgcccgggg taaaaactta taatgcaagc gatctatcat ttcagcgcta
     6361 aaaccctgtt gattgtgcat tcccatattt acgaataaat ccagggcgct ggtgtcgcga
     6421 acaatggagc gcaaaatcat cgcgacattc tttttccagg tgggattgta ttccaagaca
     6481 gagagtaaat atttggcgcg caaagcctgc ggttgaccgg attgaaaatt caaatctgtt
     6541 ttaattctcc ccttggcacg gatccattga atcaaatctg ttaaccattc gagtcttttg
     6601 cgcaataaag catcagaggg cggcggcgaa ttcaacaaag tgtcaagatt gcgccttgag
     6661 tgaggggctt ctgcccattt tgtgatcaaa tcacgagatc tttggaacat ttgctctccg
     6721 gctctgccat agttcagtcc aacaaattgc tgtcaaagtt ccagtatagc caaccacggc
     6781 tcctgctatg acatcactta aaaaatgatc atggatgatc acccgtgtaa atgcaaagaa
     6841 ggcagcaagt ataaagaata accattttcc tttggggaga gccaaggaaa gcatggttgc
     6901 caccgtaaag agcacttgcg tatggcctga tacaaatgag tgaaaatccc agtgaaatgt
     6961 gaagggatga aaaacaaagg gattaaaatc tggagattta tggggtcgct gtcgtccgaa
     7021 gcaaaatttt aagatatgaa cgaaaatccc cgaggtgagc aaagcaaaga aaagatttcg
     7081 tgaccactcg cgcagccgca gagagttggg gcgtatccaa cgtgcaaaaa aatagagcag
     7141 aatagcaata agaaaataat gttcgctcag accgacattc gtgacagcac gggcttttaa
     7201 ccaaatccct tcaagttctg gtcgtttaaa aaaaagagag agattttgat ctagagtgaa
     7261 agcaacgaga attgctaaag aaaaagcaaa tatgcttacg gtcatgatct ttcgtttgga
     7321 atatgtcatg acaccatgtt agcaaataaa aattggagtg ggtagttgca tcatacgcat
     7381 tgtctattca gaagaaaatc ataacaaata aaagcttgcc tagacaagag tccgtcgcat
     7441 taaatgaact tagatcaagt agcggcagtc aactctaaaa ggaattggaa tctatggcac
     7501 cacgaatggt tacacagctt gcttttatca atttagtttt tttaatttca ttttcagtcc
     7561 aggcttcttc gcctgattac tacacggttg aagaaacaga agcgcaaaaa ttcaatcata
     7621 gcattcatag cgattcaact gaggggttct tttccttaaa tccgatgaat gccatgtcgg
     7681 aggttgtgag agactccgct gcaaaagttt tggattttag cttaagtctc atgaactggg
     7741 ttcatcataa cgaagagttg gaagacccgg atgttcccta taatcgtaaa aaccactatg
     7801 ggacctgggt gcgcgaccgt agagaaaata attgttacaa tacgcgagcc cgcgttttga
     7861 ttcgcgactc tcagattccg gtgaccttta atgataaagg ttgcactgtc ctgagcggtc
     7921 aatggtatga tccctattca aatcaagagt acacacaagc cagcgatatt caaattgatc
     7981 acgtggttcc gttgaaaaat tcttatgtca gcggaggctg gaagtgggac tccagcacgc
     8041 gttgtctcta cgc
//
LOCUS       DATBTE010000232        15073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_850283, whole genome shotgun sequence.
ACCESSION   DATBTE010000232 DATBTE010000000
VERSION     DATBTE010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 15073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15073
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850283"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..767)
                     /locus_tag="VIG33_08490"
     CDS             complement(63..767)
                     /locus_tag="VIG33_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091792.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEY8270914.1"
                     /translation="MKIFLLAVILISSLALSAESFAEEKLIVVSSFTEKAVEPNMVFF
                     NVEVWSKAVSAKQAQSLNATEFQKIRKTLDAFKIKKEDIQTENYSLNPEYIYDQKTQQ
                     NKMMGFHALQILRVTLRKTEEMGSFLDAVITSDPQNASGVNVNSIQWDTDKRQQIELA
                     ALSDAVKNGRLKADELAKAANVKIKGVSMLSHGVAASYSPMPVAAVRGMMKENTEVMA
                     GQIKIRVDVTAHYEIQ"
     gene            complement(788..1291)
                     /locus_tag="VIG33_08495"
     CDS             complement(788..1291)
                     /locus_tag="VIG33_08495"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEY8270915.1"
                     /translation="MSKVIALIYADKKIPAEIYAEAQRLQSQGRIDLIDVTEITVKDN
                     GKIKFEQGMTLPLIGCSSGLFLPAFVGLIFFHPHYMANDNVQKMLQDISLDQNFIRVL
                     SREVLPRNSVLFLYLRNQNTSQIAAAMTRHGGRVAEISLMAGQEEKLHRLFRGKPLPD
                     PQHTMNI"
     gene            complement(1425..3692)
                     /locus_tag="VIG33_08500"
     CDS             complement(1425..3692)
                     /locus_tag="VIG33_08500"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEY8270916.1"
                     /translation="MSAKIETTPEMVRNVYAKSKERLAIVRKRLNRPLTLAEKIVFGH
                     LDDPQNQELVRGSSFLQLRPDRVAMQDATAQMALLQFMLAEKEEAAVPSTVHCDHLIQ
                     AYHGKTEDMAASNKVNEEVFNFLATSTSRYNIGFWKPGAGIIHQVILENYAFPGGLMI
                     GTDSHTPNAGGLGMCAIGVGGSDASDVMSGLAWEVKNPKLIGVHLKGQMHGWTSAKDV
                     ILKLCGMLTVKGGTDKIVEYFGEGTKSISCTGKGTITNMGAELGATCSVFPYDERMKA
                     YLASTARAELAAVADQNRDLLTADPEVALDPKKYYDEVYEIDLSALEPHLVGPHSPDI
                     ARPISAIAKEAAEKGWPTKLSSALVGSCTNSSYEDIGRATFVAQQALECGAKMKQPFL
                     VSPGSTQIQNTIQRDGQMAVLNEVGATVLANACGPCIGQWKRDDIKTGEKNTIVTSFN
                     RNFRGRNDSNPETLAFIGSPELVMALGLAGRLDFNPMVDELETPKGKVKLKAPEAPEL
                     PAKGFVPDIEGYQKPEGSTAKLAVNPASDRLQLLQPFPRWDGKDFVDQLVVAKAKGKC
                     TTDHISPGGPWLKYRGHLDNISNNMLLGADNAFTGEIGKGKNQLTGQTQEFAKIAREY
                     QKAHKGWVIIGDENYGEGSSREHAAMSPRFLGCTAVVTKSFARIHETNLKKQGVLALT
                     FANPEDYNKIKEDDHVSLLDLANMAPGKNIKMEIKHQDGSKEVIDLKHSYIPDQINWF
                     KAGSALNLIRAANKR"
     gene            complement(3808..4200)
                     /locus_tag="VIG33_08505"
     CDS             complement(3808..4200)
                     /locus_tag="VIG33_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270917.1"
                     /translation="MKKWFCLSLILISGVAQAKLSDQQSMKCINADKLTHLEFQDLQA
                     AYDMRLVSAKDFFRVMDAPNCAQLKKDLKKLHGSALAAFPQTKLSFHSHKSGSDSDFA
                     ESHTPQLGAPPPPPLSEADFDDDFSDFR"
     gene            4501..5829
                     /locus_tag="VIG33_08510"
     CDS             4501..5829
                     /locus_tag="VIG33_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270918.1"
                     /translation="MPRYIGVLIIYLFLFQLSFNALAKSSWDLTTDAKIESVYWPKSY
                     GEDTNNTLHRLELIPTLSGKFSNSFSGYFRPSFLWDPQNKSKEEQVFFDIGQAYFKYK
                     GETSSLQLGSNILNWGVTDGYNPLDVVNGRQYYDPLRSVKLGAWSLLFSHASTSSEQE
                     LIYIPRSRSSILPGTQSRWLPRQIYIPRSIENNVVLLLPENLNYTYESRETLNQALDN
                     NVGLRLQWHFNSNLGAIDLGFTGFEGVATFPLIQPKVTGTVIQISPQLILQTDPDVFL
                     RIKDYRQRVGGFSWVSSQFGFLLKYVTNYSQSLGDDPLLPGWTHENIFGLERNFNIGS
                     EGLLVGILQYSFINTEKENASNLSVTEIFRRAWMAGGRFTWGDNWTFTALGLYDSLRY
                     SHFQQYSLARRIADVWTLQLSAELISGREDTPLGVYNENDNYRLSLGRSF"
     gene            complement(5832..7349)
                     /locus_tag="VIG33_08515"
     CDS             complement(5832..7349)
                     /locus_tag="VIG33_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270919.1"
                     /translation="MLNIQIRLFIVVFICFFSAVDVFANSVVETEFAMLLPRPFQQSL
                     IEQEWQTLQKDEFKLDWGLPDQVYDTPDVKVFLTGLQLNLQTKLRKPNVNPEGESVIL
                     DSQDLAADLNIQAVAIDQYIEREIGGIIGRFHIQARCEGVQLHMKAGQGAFVMKLSPV
                     FERGLVRAHVDDVSIAWTADAWNITDMKCTGAQGFDEVVKAEILKRTKDASIMEQQKT
                     ALIGSIQNYVNERSVDLSKPRNLTSTRSDIQISMRIDNFVGTEKNALAHGVFRVEFTK
                     LSNQVKSPKYVFLKLSKQSFEDSSSTDPLLRVPEEFVVTMTKQAFAASSWLRRMNSSQ
                     LPGFSSLMESRFKQFFVWRELTNFSQATEFLFDIYSPKNVAVSGKNLKYQVKAPLQSR
                     MYAPKNGRYVPFMNFVVPFSSQVQLSVEQRKIFAKFTGVALSLQERWEPSYVERYAPS
                     RTFDRTTIGQRIQSSAEGKATSYALPPIPLTETLNLQFQKVLQPARSSDVVFYLK"
     gene            7498..8280
                     /locus_tag="VIG33_08520"
     CDS             7498..8280
                     /locus_tag="VIG33_08520"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEY8270920.1"
                     /translation="MLVFLMISFLFFGTLPARAALAAPLAMSLNTITQEISNRLKAKD
                     EEFDGTMKIIEADQSFKERGMKILRLSPSPKEHYIMVRMKIPKDLKGISLLATYKGGK
                     EEKWLYLPSNKQTRRLASTSKESGSILGSELNPEDFNISTESSAKNILKKEVKIQNQS
                     YYVIESDVSPVSKNYSKIISYVTTHEFLPNKSECYDKQGKLLKVIEFSNYKKLGNGKW
                     RAEKIKIRNVQNKRATEVALSNIKVDQELKASEFTPKALSDD"
     gene            complement(8282..8785)
                     /locus_tag="VIG33_08525"
     CDS             complement(8282..8785)
                     /locus_tag="VIG33_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270921.1"
                     /translation="MSRIPLWTSSLIISCVLLAGSGCSLFDRQLDPENAILKQSIHQK
                     VFFAPYDSVWKAAHTSIRYPIAVENQDTGFLETEYIKGVDGFLPPEVSKPPSAGIRYK
                     LVFNFAKGKTEGRESTRVTLEKKIERLRDFFSEADGLASDGLEEKTLFYRIEREIVIA
                     EALKKVQ"
     gene            complement(8757..10226)
                     /locus_tag="VIG33_08530"
     CDS             complement(8757..10226)
                     /locus_tag="VIG33_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270922.1"
                     /translation="MHLGLQLKGVVLTAAFISIHLVGCTPDKSGRFESGQPVGVIDTT
                     QTPGLNIVGEDLQPLAGAKILIGQDLNDPFLNNYMVADSSGHVDLPSGWQDAQTVTIE
                     APNYIRASYFAQTPADRTYRLRKKRSTLINQYELKGLTTGHDITDRDGLVDFSLVIPA
                     MTRNDFLSFNVSTVISPQNDKISIMGQEVGIPSNVTLPKQKESYVIPVNFDKPNYRIY
                     FGDGGTQKVFSARGRFPLKKVVDKLRNNDPFYELMNDFVISGGGVKDLNLTTSSTDAD
                     LSVKDLDFKNKVDVTAPTIDSDETLMVVGVAHMAEYMLPTDVKLIKSGEKLGITTLDT
                     KGSVFSILKKTKDMDSNDPAAERLSAVLMPALLGVSPKFLPIMQDPTLTSNGDMLVPK
                     IHPVAGVNPVATYAVISQVENLPQGAKKVKVNNSYWEMYAPHWMEVVSLPRWPEGNHM
                     AGKKHWEINLVGSLTVSQVDLGPAMINNATHVTHSSLDF"
     gene            10510..10586
                     /locus_tag="VIG33_08535"
     tRNA            10510..10586
                     /locus_tag="VIG33_08535"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10544..10546,aa:Met,seq:cat)
     gene            complement(10565..12319)
                     /locus_tag="VIG33_08540"
     CDS             complement(10565..12319)
                     /locus_tag="VIG33_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY8270923.1"
                     /translation="MKNKSNQKSQSGYKIGLYIRVSSEEQASNPEGSIKSQECRLREF
                     VIHKNSFENFGEIVELYCDAGISAKDTNRPEFQRMMEDIRGDRINMILITELSRLTRS
                     IKDFAVLVEMLEEMDCKIHSLKDNFDTSTAVGSFSMYLVANLAQFERKQIGERISANF
                     LSRSKRGLYNGGSVPLGYRVSDKRDGRLVVVQSEAETVREIFKTFLTEGTLASATKAL
                     NARKIQLMRKREGGGKLRTGQVMLESLYRMLTNKIYIGVREYKENGKVCEAKAQWEAI
                     VNEEVFRRVGEILKNNKSRKKTSPLRHPYLLSGILHCQCGHRLSGKSAHGNGGKIAYY
                     DHAWAAKIEGVSAKKGTRCDPQRVLANKLEPVVWSAVKDFLLMPEFFSEILLEAQAQT
                     ELNTPKKEIERKQNKIYEINMQLDALSERLGLLPKSVNPKHVFDQMEKLSLQKDTHEE
                     EVKSLRSKTVQENAVDIGDFESFRSVIVDLLANEADPKVKTAIIQKVVQKIIIKNEEV
                     EIYFFVGERHYKRELEISGSRPSFNPIVPKSPEKRRTSPLPVFHGNLETSQFLDLRPN
                     FLNDAGSNSLKNGSERRT"
     gene            complement(12316..12516)
                     /locus_tag="VIG33_08545"
     CDS             complement(12316..12516)
                     /locus_tag="VIG33_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270924.1"
                     /translation="MKKKWSPKFPVIKKSPVKDERFEQLLAEVGEILYKRLCQQVQKK
                     STSTEPALKELGTNQFKKAMSS"
     gene            complement(12633..13454)
                     /locus_tag="VIG33_08550"
     CDS             complement(12633..13454)
                     /locus_tag="VIG33_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270925.1"
                     /translation="MLKTIWRWKVLNYPLAKELCFKKISANNAYRKVRRLIHEGYIEE
                     ALSTKIDLYALRLTQKGFNKIKYDIGYVTQERYLPQSVSHDYWAMAFQLGEYAFNPNK
                     DVEFVTEQELQVTDSRLLPDWIPTVTNHIPDGCTKIKNAENSSYIAIEVELNAKPDLR
                     YVHAAYYFEGVNRKIDTVLWLCDGNWLMERIFKLFLKLNLKRMEIHHFVLLEDFKKYG
                     WDCQLRSGHEKNKIIRDVYAPRPHQLPGQTRPNPLSGKMHELFFSSKKSPWKPKV"
     gene            13786..14292
                     /locus_tag="VIG33_08555"
     CDS             13786..14292
                     /locus_tag="VIG33_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY8270926.1"
                     /translation="MAKRRNRGKTPARVKFSYPRKKSVFQQTTLKIGEVAKKSNIPIV
                     TLRFYEEQGLIKPARNQHKQTSHRRYKVSVTSELEFIKHCRAAGFTLPEIRSMLKIFR
                     GFKPPAKLLMNSIYRTIDRIRNQMRNLEEIERIMVMRLQNPQDDIEALIEQDPEIWRL
                     RGLKLNPD"
     gene            complement(14400..>15073)
                     /locus_tag="VIG33_08560"
     CDS             complement(14400..>15073)
                     /locus_tag="VIG33_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY8270927.1"
                     /translation="IISANYSLDSNATADTILKRNLHAPLLFALSDQNGKSVVNTSKT
                     IIGTLEFFNPSPLLLGADISMMNSALGRISDIVRVHLVDTSASPLQTSKLPSKVGDTV
                     YLVGYPSETSNRTKVGAPDSGGNSLRISVGKIISFADWKQRTGNNFGAQTESLLQENL
                     VFFDADCEHGNSGGPLLNANGEVIGIMMALYQDVQKGAPYRVCGAVNTLDESKLTALW
                     KLQAQ"
BASE COUNT         4118 a         3541 c         3123 g         4291 t
ORIGIN      
        1 ctggaataac actgcgaatg gcggaggaga gcaaccactc cccgtcccct aataaatttt
       61 aattactgta tttcataatg ggcggtcaca tcgacacgaa tttttatttg gccggccata
      121 acctcggtgt tttctttcat catgcctctg actgctgcga ctggcatcgg actataactc
      181 gccgccacac catggcttaa catcgagaca cctttaattt ttacatttgc tgctttagca
      241 agttcatcgg ctttaaggcg tccatttttg accgcatcac tgagcgctgc taactctatt
      301 tgctggcgct tgtccgtatc ccattgaatt gaattcacat tgacgccgga ggcattctga
      361 ggatccgagg tgatcaccgc atccaaaaac gaacccatct cctctgtttt ccgcaaagtc
      421 actctaagaa tttgcaatgc atgaaagccc atcattttat tttgctgagt tttttgatca
      481 taaatatatt ctggattcaa agagtaattt tcagtttgga tatcttcttt tttaatttta
      541 aacgcatcca aggtctttct tattttttga aactcagtgg cattaaggct ctgcgcttgt
      601 ttggcgctaa cggctttgct ccatacttcg acattgaaaa aaaccatatt aggctccacc
      661 gccttttccg taaaacttga aactacgatg agcttctcct cggcaaaact ttctgccgat
      721 agagccaatg aagaaataag aatgactgcc aataaaaata ttttcatgaa atcctccgat
      781 tcaaacacta aatattcata gtgtgttgag gatcaggcaa gggttttcct cggaaaagcc
      841 ggtgtagttt ctcttcttgc cccgccatta aagaaatctc agccactctt cctccatgcc
      901 gagtcatggc cgcagcgatc tgactggtat tttgatttct taaatacaaa aaaagcacgg
      961 aatttcgcgg aagaacttcc cttgaaagaa ccctaataaa gttttgatcc agagaaatgt
     1021 cctgcagcat cttttgaacg ttgtcattgg ccatataatg aggatggaaa aaaataagcc
     1081 ccacaaaagc cggtaagaaa agcccagagg agcagccaat taaagggaga gtcattccct
     1141 gttcgaactt aatttttcca ttatccttca cagtaatctc cgtcacatca atcagatcga
     1201 ttcttccttg actttgcaaa cgctgagctt cggcgtaaat ctccgcagga atttttttat
     1261 cagcataaat caaagcgata actttagaca tagaaccctc ttgcaagaat cttagaaaaa
     1321 aaagttctca tccataaggc gaaatcacga agatgcagta aaataaaaaa acccctctct
     1381 tttgaaaggg gttttttatt tatttcaact ttaagtttct aaaactatct tttgttcgcg
     1441 gcgcgaatca aattcaatgc cgatcccgct ttgaaccaat tgatttggtc aggaatatag
     1501 ctatgcttca aatcgatgac ttctttcgaa ccatcttggt gtttgatttc catttttata
     1561 tttttacctg gagccatatt cgccaaatca agtaaagaca catgatcgtc ttctttaatt
     1621 ttattgtaat cctcaggatt cgcaaaagtc agagccaaaa ccccttgttt tttcaggttg
     1681 gtctcatgaa tccgcgcgaa ggacttggtc accacagcag tgcagcccaa gaatcgaggc
     1741 gacatcgctg catgctcacg agaggaacct tcaccatagt tctcatcccc tataatcacc
     1801 caacccttat gggctttctg atactcgcga gcaatcttcg caaactcttg agtttggccg
     1861 gtgagttgat ttttaccttt accaatttcg ccagtgaaag cgttatccgc tcctaaaagc
     1921 atattgttag agatattgtc caagtgaccc cgatacttca accaggggcc acccgggctg
     1981 atatgatccg tggtgcattt gccttttgct tttgccacaa ccaattgatc cacaaaatct
     2041 ttaccatccc atcgcgggaa gggttgcaac aattgcaaac ggtccgacgc tggattcacc
     2101 gccagcttgg ccgtactgcc ttctggtttt tgataacctt caatgtctgg aacaaaaccc
     2161 tttgctggaa gctctggagc ctccggggcc tttaatttca ctttgccctt aggggtttct
     2221 aattcatcca ccatcggatt gaaatccaaa cgacctgcca agcccaaagc catgactaac
     2281 tctggagagc caatgaaggc caaagtttca ggatttgaat cattgcggcc acggaagtta
     2341 cgattgaaag aagtcacgat cgtattcttt tctccggttt taatatcatc ccgcttccat
     2401 tgaccaatgc aaggaccaca ggcattcgcc agaaccgttg cccctacttc attcaataca
     2461 gccatttggc catcacgttg aatcgtgttc tgaatttggg tagagcccgg acttaccagg
     2521 aatggctgtt tcattttcgc gccacactcc aaggcctgct gagcgacgaa agtcgcgcga
     2581 ccgatatctt cataagaaga attggtgcaa gagcccacca gcgcggaaga caatttggtc
     2641 ggccaacctt tttcagcagc ttctttggca atcgcggaga taggacgagc gatatccgga
     2701 gagtgaggcc ccactaagtg aggctctaaa gccgagagat caatttcata aacctcatca
     2761 taatattttt taggatccaa ggcgacttca ggatctgcgg tcagaaggtc cctgttttga
     2821 tcagccacag cagccaactc agcacgagcc gtcgatgcca aataagcttt catacgttca
     2881 tcataaggga atactgagca agtcgctccc agctcggccc ccatattggt gatggttcct
     2941 ttaccggtac aagaaatgga ttttgtacct tcaccgaagt actcaacgat tttatctgtt
     3001 ccgcctttga ccgtgagcat tccgcaaagt ttcaagatga cgtctttagc ggaagtccat
     3061 ccatgcattt ggcctttaag gtgaactcca ataagtttgg gattttttac ttcccaagcc
     3121 aaacctgaca tcacatcaga agcatcagat ccgcccacac caatcgcgca cattcccaaa
     3181 ccgcccgcat tcggagtgtg agaatctgtt ccaatcatca atcccccggg aaaggcgtaa
     3241 ttttcaagaa tgacttgatg aatgattccg gcacctggtt tccagaagcc aatattgtaa
     3301 cgagacgttg aggtggccaa gaagttgaaa acctcttcat tcaccttatt agaggccgcc
     3361 atatcttcgg ttttcccatg gtaagcttga atcaaatgat cacagtgaac cgttgaaggg
     3421 actgcagctt cctctttttc cgccagcatg aactgcaaaa gagccatctg cgccgtggca
     3481 tcttgcattg ctacgcgatc tgggcgaagt tgcaagaatg aagacccccg aaccagttct
     3541 tgattttgcg gatcatccaa gtgaccgaaa acaatttttt cagccaaagt cagaggacga
     3601 tttaaacgct tacgaacaat cgctaaacgc tctttagatt ttgcataaac atttctgacc
     3661 atttctggag tggtctcgat ttttgccgac atagaagcct ctttcgagtt gatttattga
     3721 catggatttt gcgtctcaca tggtaatgaa aatcagctga aagctcaatc cttagaatgg
     3781 cttacgcaag acgtcgatag cccaatccta acggaaatcg ctaaaatcat catcaaaatc
     3841 ggcctcagac agggggggag gaggaggcgc ccccaattgt ggggtgtgac tttcagcaaa
     3901 atcgctgtca gagccactct tgtgagaatg aaaacttaat ttagtctgag gaaaagcagc
     3961 cagcgcgctg ccatgaagct ttttaaggtc ttttttcaat tgagcgcaat tgggcgcatc
     4021 catgactcga aaaaaatcct tcgcgctcac taagcgcata tcataagcgg cttgaagatc
     4081 ctgaaactcc aaatgggtaa gcttatccgc attaatgcat ttcatggact gctggtcgct
     4141 caacttcgct tgagcaactc ccgagatcag aatcaatgac aagcaaaacc acttcttcat
     4201 aacatctcca aatacgtcca acaaattaac gaagcccgct cacgaaagca aatatcttat
     4261 tttgcatccc cattttttaa aagctctaga ttttttagcg acattcgtgc cacagagagc
     4321 agtgggtata ttgcaaaagg agcttaaaag gtgtgtaaga ttctcattat aggacgctcg
     4381 cagtctctgt ctaaaagttc gacacaattg acttctttta tcgcttttta tcgctaaagc
     4441 aggataaagt cttctcatct catagatagc cagaagctta cccctatgtt tctttaaacc
     4501 atgcctcgct acatcggcgt tctcattatt tacttatttt tatttcagct ttcttttaat
     4561 gctttggcta aaagttcctg ggacctcacg acggacgcca aaattgaaag tgtctactgg
     4621 cccaaatcct atggcgaaga tacgaataac accctccatc gtctggaatt gattccgacc
     4681 ctcagtggaa agttctcgaa ttcctttagc ggctacttta gaccctcctt tttatgggat
     4741 ccgcaaaata agtctaaaga agagcaagtt ttttttgata tcggtcaagc ttactttaaa
     4801 tacaaaggtg agacttccag cctccaactc ggcagcaata ttctcaactg gggagtcact
     4861 gatgggtaca accccttaga cgtggtcaac ggccgtcaat attatgaccc tcttcgttcg
     4921 gtgaagctcg gggcctggtc attgcttttc tcccacgctt cgacaagcag tgagcaggaa
     4981 ctgatttaca tccctcgaag tagaagttcg attttaccag ggactcagag tcgttggctg
     5041 ccacggcaaa tttatattcc tcgcagcatt gagaacaatg tcgtgctgct tttgcccgag
     5101 aacttgaatt acacttatga aagtcgcgag actctgaatc aagctctcga caataatgta
     5161 ggtctgagat tgcaatggca tttcaatagc aacctggggg ccattgatct gggtttcact
     5221 ggttttgaag gcgtcgccac ctttcctctc atccaaccga aagtcaccgg tacagtcatt
     5281 caaatttcac cccaactcat tttgcaaacg gatcccgatg tctttttgag aatcaaggat
     5341 tatcgccagc gagtgggagg tttttcttgg gtgagttccc agttcgggtt tctcttaaag
     5401 tacgtcacaa attattcgca aagcctcggc gacgatcccc tcttgcctgg gtggacccat
     5461 gaaaacattt ttggcctcga aagaaacttc aatattggca gcgaaggact attggtagga
     5521 attcttcaat actcctttat aaacacggaa aaagaaaatg cctccaatct ctcggtgacc
     5581 gaaatctttc gccgggcgtg gatggccgga ggtcgcttca cctggggaga caactggacg
     5641 ttcacggccc tgggcctcta tgattccctt cggtatagtc attttcagca atattccctc
     5701 gcccgacgaa ttgcggatgt ctggactctg caactcagcg ctgaacttat ctcaggccgt
     5761 gaagacactc cgttgggagt ctataacgaa aatgacaact atcgcctctc gctaggtcgc
     5821 agtttttagc cctacttcag ataaaacacc acgtccgagc ttcgcgctgg ttgcaggacc
     5881 ttttgaaact gcagattcag agtctctgtc agcggaatcg gcggcagagc ataagacgtc
     5941 gcctttcctt ctgccgaact ttgtatcctc tgccctatcg tggtccgatc aaaagtccga
     6001 gagggagcat atcgctcgac ataagaaggc tcccagcgtt cttgcaaact cagagcaact
     6061 ccggtgaatt ttgcaaagat ctttctctgc tccaccgaca gctgcacttg gcttgaaaag
     6121 ggaactacaa aattcataaa tggaacatag cgaccatttt taggagcata catccgggat
     6181 tgcaaaggag ctttgacttg gtattttaaa ttctttcccg aaaccgcaac atttttcggt
     6241 gagtaaatat caaataaaaa ctccgtggct tgagaaaaat tcgtcagctc gcgccacaca
     6301 aaaaattgct taaagcgaga ctccatcagt gaagaaaaac ccggcaattg actggagttc
     6361 attctccgca gccaagagct ggccgcgaac gcttgcttcg tcatggtgac cacaaattct
     6421 tccggcacgc gcagcaaggg gtccgtggag gaactgtctt cgaaactttg tttggaaagc
     6481 tttaagaaaa cgtattttgg gctctttact tgattgctta actttgtaaa ctcgacacga
     6541 aaaactccat gggccaaagc atttttttct gttcccacga agttatcgat gcgcatggaa
     6601 atctggatat cagaacgtgt gctggttaga tttcgaggtt ttgacaaatc aaccgagcgt
     6661 tcgttcacat agttctggat agatcctatc aaggccgttt tctgttgctc catgatagaa
     6721 gcatctttgg ttcgctttaa aatttccgct ttgacgactt catcaaaacc ttgagctccg
     6781 gtgcacttca tatccgtaat attccacgcg tcagccgtcc aggcgatgct cacgtcatca
     6841 acatgggctc gcaccaggcc tcgctcaaac acggggctga gtttcatgac aaaggctccc
     6901 tgaccggcct tcatgtgaag ctgcactcct tcacaacggg cttggatatg aaaacggcca
     6961 atgataccgc caatttccct ttcgatatat tggtctatcg ccaccgcctg aatatttaaa
     7021 tcagcagcga ggtcttggga atcaagaatg acgctttcgc cttcagggtt cacattgggt
     7081 tttcgcaatt tcgtttgcaa attcagctgt aagccagtca aaaaaacttt cacatcgggt
     7141 gtatcataaa cttgatcagg gagcccccag tccagtttaa attcatcttt ttgcagggtt
     7201 tgccactctt gttcaataag gctttgttga aaaggtcgtg gcaacagcat cgcaaattct
     7261 gtttccacaa cggaatttgc gaagacatca accgcagaaa aaaaacaaat aaaaacaact
     7321 ataaaaagtc taatttgaat attcagcatc ttgttccccg ggaaaggctg taatagcatg
     7381 attaataatc catcctgatt cacctctgtt gttaacggct tgtttctcca tttctgtagt
     7441 acaattttaa ttcagcaaaa aaataaaatc ttaaacaaac aactgaaggg gacattcatg
     7501 ctggtctttt taatgataag ctttttattt ttcggcactc tccccgcccg cgccgctctc
     7561 gcggccccgc ttgcaatgag cctcaatacg atcacacaag aaatctccaa ccgtctcaaa
     7621 gccaaagatg aagagttcga tggcacgatg aaaattatcg aggcagatca aagttttaaa
     7681 gagcgcggta tgaaaatcct gcgcctcagt ccttcaccaa aggaacacta cataatggtg
     7741 cggatgaaaa tccctaaaga ccttaaaggc atttctttac tggccaccta caagggaggc
     7801 aaagaagaaa aatggctcta tcttccttca aacaaacaaa ctcgccgtct agcctcgact
     7861 tcgaaagaat ccggttccat cctgggctct gaactgaacc cggaggattt caatatcagt
     7921 accgaaagca gcgctaaaaa tatcctcaaa aaggaagtga aaatacaaaa tcagagctat
     7981 tatgtcattg aaagcgacgt gagcccggtt tccaaaaatt actctaagat tatcagctac
     8041 gtgacgaccc acgagttttt gccgaataaa tcggaatgct atgacaaaca gggcaagctc
     8101 cttaaagtga ttgaattcag taattacaaa aagttgggga atggcaaatg gcgtgccgaa
     8161 aaaatcaaga tccgcaatgt gcaaaataag cgtgcgacgg aagtggcgct ctcaaatatt
     8221 aaagtggatc aagaattaaa agcttccgag tttacaccga aagctttgag cgatgattga
     8281 gctactgcac ttttttaaga gcctcggcaa tgacaatttc gcgttcgatg cgataaaaaa
     8341 gtgttttttc ctccaaccca tcggaagcca atccatcggc ttccgagaaa aaatcccgca
     8401 gtctttcgat tttcttttcc agagtcacgc gagtggactc tcgtccttcc gttttcccct
     8461 tcgcaaaatt aaatactaat ttgtagcgga ttcccgcgga aggcggcttg gaaacttccg
     8521 gtggcaagaa gccgtccact cctttgatat attctgtttc taaaaaacct gtatcttggt
     8581 tttccaccgc aatgggatac ctgatcgaag tgtgagctgc cttccacaca ctatcataag
     8641 gtgcgaaaaa aactttttga tgaatggatt gcttaagaat ggcattttca gggtctagtt
     8701 gccggtcgaa aagactgcaa ccagaccctg caagcaaaac acaactaatg atgagactag
     8761 aagtccaaag aggaatgcgt gacatgagta gcattattga tcatggcggg gccgagatca
     8821 acttgagaca cagtgagact tccaaccaaa tttatctccc aatgtttttt gccagccatg
     8881 tgattgcctt ctggccatcg gggcaaactg accacttcca tccaatgggg cgcatacatt
     8941 tcccaatagc tgttattcac cttcactttc ttagctcctt gaggaaggtt ctcaacctga
     9001 gaaatcacgg cataagtcgc cactgggttc actcctgcga caggatgtat tttaggcact
     9061 aacatatcgc cattggacgt cagagtggga tcttgcataa ttggtaagaa tttcggagac
     9121 acccccagga gagccggcat caacaccgcg ctcaaacgct cggctgcagg atcattggaa
     9181 tccatgtcct tggttttctt cagaatagaa aacacagagc ccttggtatc caaggtggta
     9241 atgcctaatt tttctccaga ttttataagt ttcacatcgg ttggcagcat atattcagcc
     9301 atatgagcca cacccaccac catcagggtc tcgtcactgt caattgtggg agccgtgacg
     9361 tccactttgt ttttaaaatc aagatctttc acggaaaggt cggcatccgt actggaagtg
     9421 gtgaggttga ggtcttttac ccccccgcct gaaatcacaa aatcattcat gagttcatag
     9481 aaggggtcgt tattgcgaag cttgtcgacc acttttttaa gcggaaatcg tccccgagca
     9541 gaaaaaactt tttgcgtccc gccatcacca aagtaaatgc gataattggg tttatcgaaa
     9601 ttcactggga taacgtaaga ttctttttgt ttcggcagag tcacattgct gggaataccg
     9661 acctcttgtc ccataatgct gatcttatca ttttgcgggc taatcacggt actcacattg
     9721 aagctcaaga aatcattccg agtcatcgcc gggatgacca ggctgaaatc cacaagccca
     9781 tcccgatcgg tgatatcgtg gccggtggtt aagcccttca attcatactg atttatgaga
     9841 gtgctgcgtt tttttcgcag acgataagtc cgatcagcgg gagtctgagc aaaataactg
     9901 gctctgatat agttcggagc ctcaatcgtc acggtttgcg catcttgcca tccgctgggc
     9961 agatctacgt gtcctgaact atccgcgacc atataattgt tcaaaaaagg atcattgagg
    10021 tcttggccga tgaggatttt tgctccagcc aacggctgaa gatcttcgcc gacgatattc
    10081 aaaccgggag tctgagttgt gtctatgact cccaccggtt ggccactttc gaatctgcct
    10141 gatttatccg gcgtacagcc gacaaggtga atggatatga atgctgctgt gagaacaaca
    10201 cctttgagtt gaagtcctag atgcatagcg agggtccccc ttcctttagt ggatcctatg
    10261 gttgctttat ttttgagcaa cttccgggaa atatgtttcc aagaagtcac agcttcctct
    10321 gactttttcc ctcaatagaa aaagctttgc tttcaattgc atttgagaga gggtactttc
    10381 gtcaatacta aagtctattc tgtagaattt tttatgtttg acttaagcgt cacgtgtcgc
    10441 tagaaaagtt tctcctcgaa gaaatgaagc cgcagcggaa acgcagcgtg cctaattttt
    10501 taaggatgtg gtggggtagc tcagctggtt agagcaacgg aatcataatc cgtaggtcgg
    10561 cggttcaagt ccgcctctca ctaccatttt tcaagctgtt cgaaccagcg tcattcaaaa
    10621 agttcggcct caaatctaaa aattgagaag tttccaggtt cccgtgaaaa acagggagtg
    10681 gagacgtgcg tcttttttct ggggatttag gtacaatcgg attaaatgaa ggacgggagc
    10741 ccgaaatctc gagctcccgc ttgtaatgtc tttcgccaac gaagaagtag atctccactt
    10801 cctcgttttt gatgataatt ttttggacca ccttttggat gatggcggtt tttacttttg
    10861 gatcagcttc gtttgcaagt aggtctacta tgacggatct aaaggattcg aaatcaccga
    10921 tatcaacggc attttcttgc actgtcttcg atcttaaaga tttcacttcc tcttcgtgag
    10981 tgtccttttg aagggaaagt ttttccattt gatcaaagac atgcttagga tttactgact
    11041 ttggcaaaag ccccaggcgt tcactaaggg catcaagctg catattgatt tcgtagatct
    11101 tgttctgctt tcgctcgatc tcctttttgg gggtgtttaa ctctgtctgt gcttgagctt
    11161 ctaaaaggat ttcactgaaa aactccggca tcagcagaaa atctttcacc gcactccaga
    11221 caacgggctc aagtttattg gctaaaaccc gttgcggatc acacctggtc cctttcttgg
    11281 cactcacgcc ttcaatcttt gcggcccacg cgtggtcata gtaggcaatc tttccgccgt
    11341 taccatgggc ggacttccca gaaagcctgt ggccacactg acagtgcaaa ataccagaga
    11401 gcaaataagg atgccttaag ggagaagttt tctttctgga tttattattc tttaagattt
    11461 ccccaactcg cctaaaaacc tcttcattta caatcgcttc ccattgagcc ttagcctcgc
    11521 aaaccttacc gttttctttg tactctcgaa ctccaatata aatcttattc gtgagcattc
    11581 ggtaaaggct ttccagcatc acttggcccg tgcggagctt tccgccgcct tctcgtttac
    11641 gcataagctg aattttccgg gcattcagag ccttcgtggc ggaagccaac gtgccttccg
    11701 tcaaaaaggt tttaaagatc tcccggacag tttcagcctc actttgcaca accacaagcc
    11761 tgccatctcg cttgtcgctc acgcgataac ccaacggcac actcccgccg ttataaagac
    11821 cgcgctttga tcggcttaag aagttggctg aaatcctttc cccaatttgc tttcgctcaa
    11881 actgagccaa atttgcaacg agatacatgg aaaaagaacc caccgccgtt gaggtgtcga
    11941 aattatcttt tagagagtgg atcttgcaat ccatctcctc taacatttca accaaaaccg
    12001 caaagtcctt aatggaacgc gtgaggcggg aaagctctgt gatcaaaatc atattgattc
    12061 tgtcaccacg aatatcctcc atcatgcgct gaaattccgg tctattggtg tctttggctg
    12121 agatcccggc gtcacaataa agctcgacga tctcgccaaa gttctcaaaa gagtttttgt
    12181 gtattacaaa ctcccttaat cgacactcct gggatttgat cgagccttcg ggattactgg
    12241 cctgttcttc ggatgagacc cggatgtaaa gaccgatctt gtaaccgctt tgggatttct
    12301 ggttgctttt gtttttcatg aggacatcgc ctttttaaat tggttagttc ccaattcttt
    12361 taaagcgggt tcagtggatg tacttttctt ttggacttgc tggcagagtc gtttgtataa
    12421 aatttctccg acctcggcaa gcaattgctc aaagcgttca tctttaacag gactcttctt
    12481 aataacagga aacttgggac tccatttttt cttcatggct ccctccgata caaaataatg
    12541 atttaattat tgtgtgctat gcacgagtta taaaccgcca tgatacccaa ttgggtatag
    12601 tgttaaaaac tggcgacaaa ttcatttcga ccttaaacct ttggtttcca tggacttttc
    12661 tttgaagaga agaagagctc atgcattttt cctgataagg gatttgggcg ggtctgacca
    12721 ggcaattggt ggggccttgg tgcatacacg tcacggatga ttttgttttt ttcatgtccg
    12781 gatcttaatt ggcaatccca tccatatttt ttaaaatcct ccaaaagtac gaagtgatga
    12841 atttccatcc gcttgaggtt taattttaaa aataatttaa atattctttc catcaaccag
    12901 ttgccgtcac aaagccaaag aactgtgtct atttttcgat taactccttc aaaataatag
    12961 gccgcatgaa catagcgcag gtcgggctta gcatttaatt ccacttcgat agcaatatat
    13021 gaactatttt ctgcattctt tattttggtg caaccgtccg gaatgtgatt cgtaactgtt
    13081 ggaatccagt ccggcaaaag tctgctatca gtgacctgaa gttcttgctc agtcacaaat
    13141 tcaacatctt tatttggatt aaaagcatat tctcccaatt gaaatgccat ggcccaataa
    13201 tcatgagaca cactttgcgg taaatatctt tcttgagtca cataaccaat gtcatattta
    13261 attttattaa atcctttctg cgtaagccgt agggcataaa gatctatttt tgtactgagg
    13321 gcctcttcta tatagccctc gtggataagt cttctgactt ttctgtaagc attgtttgct
    13381 gatatttttt taaaacaaag ttcttttgct aacggatagt ttaagacttt ccagcgccag
    13441 attgtcttta gcaagaaaaa ctccggctgc cttaagcgcc gcagtttatt gtattttctt
    13501 ttcttttttc caattctttt ttgagacgat gattttatcg ccaaatcaat gtctggtata
    13561 aaggacctcc aggtgaaaag ttgtatcgcc ccgccagcag gcagggtgtt gagcacaaaa
    13621 gttttttctt tattggaatt taaattcgag aaatccctgt aaaggactgg tttttcctat
    13681 gagaacttaa caatttcaga ctcttatagt aggttgagta aattctttgc acatcttcct
    13741 aatctatatc gtgacaaaag ctatagttaa atatagattt taaaaatggc aaaaagaaga
    13801 aatcgcggaa aaacacctgc gcgagtcaaa tttagctatc cacgcaaaaa atcagttttt
    13861 cagcagacca cactgaagat tggtgaggtg gccaagaaat cgaatatacc aattgtgacc
    13921 ctcaggtttt atgaagaaca aggactcatc aagcctgccc gtaaccaaca taagcagaca
    13981 tcccatcgcc ggtataaagt ttctgtgact tcagagcttg agtttataaa gcattgccgg
    14041 gcggctggct tcacgcttcc tgagatccgt tctatgctga aaatatttcg ggggtttaaa
    14101 ccaccagcaa agcttttaat gaattcaatc tatcgcacaa ttgatcgaat acggaatcag
    14161 atgagaaacc ttgaggagat agagcgcatt atggtgatgc gcttgcaaaa tcctcaggac
    14221 gacatagagg ccttgattga acaagacccc gagatttggc gtttgcgtgg attaaagctc
    14281 aatccggatt gagattgcac gggaaagcga acctttgcgc tcagggcgaa aacgaaaggg
    14341 tcagttgaat gtcccttttt gatggggatt agaagaaaac caagtgtttt tgaagctcct
    14401 tattgagctt gcaatttcca caacgctgtg agtttggatt cgtctaaagt gttaaccgct
    14461 ccacaaacgc ggtagggggc tcccttttgt acatcctgat acaaagccat catgataccg
    14521 ataacttcac cattggcatt taagagcggg cctccgctat taccgtgctc acaatctgca
    14581 tcaaaaaaaa ccaagttttc ttgtaaaaga ctttctgttt gagctccaaa attatttcca
    14641 gttcgctgtt tccaatcagc aaaagaaata attttcccaa cagaaattct caaagaattt
    14701 ccgccagaat cgggagcgcc gactttcgtt ctatttgatg tttctgacgg atatccgacc
    14761 aaataaacgg tatctcccac tttagatggc aattttgaag tctgcagtgg cgatgctgaa
    14821 gtatcaacta gatggactcg aacgatgtcg ctaattcttc ccaatgcgct attcatcatc
    14881 gagatgtctg cacctagcaa aagaggtgat ggattgaaaa actcaagcgt tccaataatt
    14941 gtctttgaag tatttacgac cgattttcca ttttgatcgc tgagagcaaa taataaaggc
    15001 gcatgcaaat tccgttttaa aatagtgtca gccgttgcgt tgctatccag cgaataattt
    15061 gcagaaatta ttt
//
LOCUS       DATBTE010000233         4919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1737631, whole genome shotgun sequence.
ACCESSION   DATBTE010000233 DATBTE010000000
VERSION     DATBTE010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737631"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="VIG33_08565"
     CDS             <1..1090
                     /locus_tag="VIG33_08565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HEY8270928.1"
                     /translation="AYKMAYESVLLYSKHACSQFRVKTFEGRCEIGLLLRSYMGIYTL
                     TPNPVLDLGGIVDKLIPNEKSYVHNETRCPGGNGINAARILHRLGSPVTLTGFLGGGV
                     GQELRLLLNQESLNHKFINIQGSTRIGITVSEKDSHLQTRLSFPGPMIQSPERMALFE
                     FFSKIKKPAIVVIGGSLPDGISVRDLQEIINLCQSKNIFTIVDCPAPILKKILARETL
                     MIKPNLHEFQEAVGRKLRSAESVVREARKFSKKIPLICVSSVEQGALLVTPRFAWYGE
                     IPKVKIRSVVGAGDSMVGAIVAQLWKHKILLKDNFEEFELHQSAELLRWGLAAACATL
                     THPGTTLGSREQIESFYSKINIEPVKSN"
     gene            complement(1087..1434)
                     /locus_tag="VIG33_08570"
     CDS             complement(1087..1434)
                     /locus_tag="VIG33_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270929.1"
                     /translation="MEKINSLDILEAGTGFMFSLSEALHQLEKKGYSENLSAKTDHFE
                     CRSGRFKMYPKDIRVDGIARFENTSDPDDQSILYAISAPSQGLKGVYVESFGIHQDEL
                     SKEMIERLKNHPH"
     gene            1592..2587
                     /gene="prs"
                     /locus_tag="VIG33_08575"
     CDS             1592..2587
                     /gene="prs"
                     /locus_tag="VIG33_08575"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEY8270930.1"
                     /translation="MQRNLSNTPESLPKIFGMGSSQLLAQELCDLSGSEIQQIQAEKF
                     SDGEVKLTPMAAVPRENIWIIHSMAFESAKSTGDKIWELLMLISLLRDEGAGPITAVL
                     PYFSYSRSDQKKEAQDPLTLRYLAQLYEAAGIQRIVTVDIHNLAAFQNAFRCPSINLE
                     ASSLFCDYLSTKHFPDIPLIVMSPDLGGLKRAEKFCRDLQTALSRSQPRSISMAIVEK
                     YREKEGLRGQALVGAVKDAHVFIYDDIISTGKTVLRAADLARQHGAKFVTVMATHGLF
                     SEDVEKVLRAPCIDEFVVTNSNLSLLDPKFKEFPKLKILSCVPILAQGLGIMGIS"
     gene            complement(2557..3246)
                     /locus_tag="VIG33_08580"
     CDS             complement(2557..3246)
                     /locus_tag="VIG33_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY8270931.1"
                     /translation="MKSEKSELNKQEKEVMINYKDLRLNGILALPPAAQGIVIFAHGS
                     GSSRWSTRNNFVARQLQKVGLGTLLMDLLLPEEEDERDNVFDIELLAERLISAKNWLK
                     QQAPTRHLPVGYFGASTGAGAALFAAALQPQNIFSVVSRGGRPDLAGAKLSHVQAPTL
                     LIVGGEDTIVIDLNKQAYSHLNCKKKLEIIPGATHLFEEPGTLGQVANLASNWFTKYL
                     EAQEIPIIPNP"
     gene            3404..4174
                     /locus_tag="VIG33_08585"
     CDS             3404..4174
                     /locus_tag="VIG33_08585"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY8270932.1"
                     /translation="MSIKKKIILFLSLAILFLGIAFFAIFFKPREYLKPKRGEVVETI
                     YGLGTVTADQTYHMRSGITLSIRKLFVKEGDFVKVSQPLAQLDQSIMRSPIEGTVTVV
                     AYKEGEIVPPQGSIIMVTNLKHLYLEVSLEQQSVLPVKENQKVFISFESLRNERVEGW
                     VKSIYPRENQFIVRIELKNWPSGVLPGMTADVAILVGKKSDALLIPLRAISAGQVERL
                     RNGKKEKVSVKLGVIDGEWGEVVSDNISVDDELLIRKK"
     gene            4178..>4919
                     /locus_tag="VIG33_08590"
     CDS             4178..>4919
                     /locus_tag="VIG33_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8270933.1"
                     /translation="MFFLAIRHLLSRRRQTGLTLLGIILGAAAYIAISGMMLGFQAFI
                     VDQLVNNDSHIRINAREEILTEKSLNTAFFDPDVLIRWLKPPSGRKDNAYILSPRSWL
                     DRLEKNPIVLAASPQLVVQAIATYAKVTAGVRIIGSEPDKQRQVANIENYMLKGKFSD
                     IGSTGNRIAVGKDFLTKIGAVQGESIFLTVGKGSPQPFKIVSVFQLGVKPLDEGTIFG
                     ALSDVQKLNQTPSRISDIAIRLTDVQKAR"
BASE COUNT         1377 a          956 c         1129 g         1457 t
ORIGIN      
        1 ggcttataag atggcatatg agtctgtatt gttatacagc aagcatgctt gtagtcaatt
       61 tagagtgaaa acattcgaag gtcgatgcga aattggcctc ctattaagga gctacatggg
      121 aatttatact ttaactccaa atcctgttct agatcttggt ggaatagttg ataagttaat
      181 accgaatgaa aagtcctatg ttcacaatga aacaaggtgt ccaggtggga atggaattaa
      241 cgctgcgaga atccttcacc gtttgggttc gccagtcacg ctcaccggtt ttctcggagg
      301 cggtgtaggt caagaattaa gactccttct gaatcaagaa agccttaacc ataaatttat
      361 aaatattcaa ggcagcactc ggattgggat tacagtatcg gaaaaggatt cgcatctaca
      421 aacgcgcttg agttttccag gtccgatgat ccaatcccca gaacgaatgg cactctttga
      481 atttttttcg aaaattaaaa aaccagctat agtggtgatc ggcgggagtc tcccggacgg
      541 aatttcagtg cgggatcttc aagagatcat caacctttgc caaagtaaaa atattttcac
      601 catcgttgat tgcccggcgc ctattctaaa aaaaatattg gctcgagaaa cactgatgat
      661 caaaccaaac cttcatgagt ttcaagaagc tgtcggtcga aaattacgtt ctgcggagag
      721 tgtcgttcgc gaagctagaa agttttccaa aaaaattcct ctcatttgcg tctcttcggt
      781 agagcaagga gccttgttgg tgactccgcg ctttgcgtgg tatggtgaaa ttcccaaagt
      841 gaaaattcgc tccgtggtcg gcgcaggtga ttcaatggtg ggcgccattg ttgcccagct
      901 ttggaaacac aaaattttgc ttaaagataa ttttgaggaa ttcgagttgc atcaatctgc
      961 agaacttctt cgttggggcc tggctgccgc ttgtgccacc ttgactcatc cgggaacaac
     1021 tttgggaagt cgggaacaaa ttgaatcatt ttattcaaaa attaacatag agcctgtaaa
     1081 atcaaattaa tgcgggtggt ttttcagtcg ttcaatcatt tctttcgata actcatcttg
     1141 gtgaattccg aaggattcga cataaacacc tttgaggcct tgggaaggtg cggagatggc
     1201 atagaggatg gattggtcat caggatcaga ggtgttttca aagcgagcaa ttccatcgac
     1261 acggatgtct tttggataca tcttaaaccg tcctgaacga cattcaaagt gatctgtttt
     1321 tgcgcttaag ttttccgagt aacctttttt ttcaagctgg tgaagcgcct cactcaggct
     1381 aaacataaat cccgttcctg cttcaagaat atctagggaa ttaatttttt ccataaaaat
     1441 acctcttatt ttattctatc attatttttt tactgaagag atgcttctta tcagtcgcat
     1501 atgtaatgaa atatttgaag taagtttatg tcaaaatacc tagttcattt cgctttaaga
     1561 agatctagaa tcagccggcg gagatttaaa aatgcaaaga aatctaagca atacgccgga
     1621 gtctctccct aaaattttcg gaatgggaag cagtcagctt ttagcgcagg aactctgtga
     1681 cctatctgga tctgagattc agcaaatcca agcagaaaag ttttctgacg gagaagtaaa
     1741 attgactccc atggctgccg tacctcgaga aaatatttgg attattcact caatggcctt
     1801 tgaatcggcg aaaagcacag gcgataaaat ttgggagctt cttatgctta taagtctttt
     1861 gagagatgaa ggggcaggtc ccattacagc ggtcttgcct tatttctctt attcccgttc
     1921 cgatcaaaaa aaagaagcgc aggatcccct cacacttcga tatctagcac agctttacga
     1981 agctgcaggt attcaaagga ttgtgactgt tgatatacat aacctcgccg cttttcaaaa
     2041 cgcctttcgg tgtcccagca ttaatttgga agcctcttct ttgttttgtg attatctaag
     2101 taccaaacat tttcctgata ttcccctgat agtgatgtcc ccggacttag gaggactgaa
     2161 gagggcagag aagttttgca gagatctgca aacggcattg tcgagatcac agcctcgatc
     2221 aatatcaatg gcgatcgtcg agaagtatcg cgagaaagaa gggctcaggg gacaagcttt
     2281 ggtcggagct gtcaaagatg ctcatgtttt tatatatgac gatattatca gtactggaaa
     2341 aacagtgcta agggcggcgg acttggcccg tcagcatgga gcaaagtttg tgacagtcat
     2401 ggctacccat ggattatttt ctgaagatgt cgaaaaagtt ttacgcgctc cttgtattga
     2461 tgaattcgtg gttacaaatt ctaatctttc cttgcttgat cctaaattca aggaatttcc
     2521 aaaattgaaa attctctcct gtgttccgat tttagctcaa ggattgggaa ttatgggtat
     2581 ttcctgagct tctaagtatt tagtaaacca gttcgaagcc aaatttgcca cttgtcccaa
     2641 tgtgccaggt tcttcaaaga gatgggtggc tcccggaata atttctaatt tctttttgca
     2701 attcaaatga gaataagctt gtttgttcaa atcaatgacg atggtatctt cgcctccaac
     2761 aattaacaat gtaggggctt gaacatgaga gagttttgcg ccagcaagat cggggcgtcc
     2821 tcctcgagag acaacagaaa aaatattttg tggctgtagg gctgctgcga aaagagcggc
     2881 gccggctcca gtgctagcac caaaatagcc gactgggaga tgcctggttg gagcttgttg
     2941 cttaagccaa ttttttgcag agataagtct ttcagcgaga agttctatat cgaaaacatt
     3001 atcccgttca tcttcttctt ccggaagaag gagatccatg agcaatgttc cgaggcctac
     3061 tttttgtaat tgccgggcta cgaaattgtt acgagtactc cagcggctgc ttccgcttcc
     3121 atgggcaaaa atgacaatgc cctgtgcggc agggggtaag gccaggatcc cattgaggcg
     3181 aagatccttg taattaatca tcacttcttt ttcttgtttg ttcagttcag atttttctga
     3241 tttcattttt atttccttgg taatttgttt gccccatcct ccaagaggcc agtctaatga
     3301 tgagcgccga aattctcagc ttgtttttag gcagggatgt ttgtaaataa caaggtgatg
     3361 ataacgtatt gtatttcact acttggttta ctagtattag tttatgagta taaagaaaaa
     3421 aattatttta tttctaagcc tggcgatttt atttcttgga atagcgtttt ttgcgatttt
     3481 ttttaaaccc agggaatatc taaaacctaa gcgtggtgaa gtcgtagaga ccatctatgg
     3541 attggggacg gtaactgcag atcagaccta tcacatgcga agtggaataa ccctttcaat
     3601 acgtaagctt tttgttaaag aaggggattt tgtgaaagtg agccagccgc tggcgcaact
     3661 agatcaatcc atcatgcgct ctccaatcga aggcacggtc actgtggttg catataaaga
     3721 gggtgaaatc gttccaccac agggttctat tattatggtc actaacctta aacatctgta
     3781 tttggaagtg agcctcgaac agcagtctgt tttgcctgtc aaagaaaacc agaaagtctt
     3841 catatctttt gaaagcttgc gtaacgaaag agtggaggga tgggttaaat ccatttatcc
     3901 tcgtgaaaat caatttatcg ttcgtattga gttaaagaat tggccttccg gagtcttacc
     3961 aggaatgaca gcggacgtcg ccattttagt gggaaaaaaa tcagatgctt tattaattcc
     4021 tctcagagca atttctgccg gtcaagtaga gcgtctacgt aacggaaaaa aagaaaaagt
     4081 ttcagtgaaa ttaggcgtga ttgatgggga gtggggagaa gttgtttctg ataatatttc
     4141 agtcgacgat gagttgctga ttcgaaagaa gtaagtcatg ttttttttgg ccattcgtca
     4201 tttattgagt cgcaggcggc agacaggcct cacgttgttg ggaattatcc taggggcggc
     4261 ggcttatatt gcaatttccg gtatgatgtt gggatttcag gcattcatag tggatcagtt
     4321 ggtcaacaat gattcccata tccgaatcaa tgcacgtgag gagatactga cagagaagag
     4381 cttgaatact gcgttttttg atcccgacgt cttgattcgc tggcttaaac ctccttctgg
     4441 acgcaaagat aatgcttata ttttatctcc gagatcctgg ttggatcgat tggaaaagaa
     4501 cccgattgtc ttagcagcgt ctccgcaact tgtagtccag gctatcgcca cctatgcaaa
     4561 ggtgacggcg ggcgttcgta ttattggttc tgagccagac aaacagcgac aagtggccaa
     4621 tatagaaaat tatatgttaa agggaaaatt ttcggatatc ggttccaccg gaaatagaat
     4681 tgctgtggga aaagatttct taactaaaat tggcgcggtt cagggggaaa gcattttttt
     4741 gacggtcgga aagggcagtc ctcaaccttt caaaattgtc agcgttttcc agttaggagt
     4801 gaaacctctt gatgaaggaa ctatttttgg agctctcagt gatgtgcaaa aactcaatca
     4861 aactccgagt cgaatatcgg acattgccat tcgcttgaca gatgttcaga aggcccgag
//
LOCUS       DATBTE010000234         1864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_987296, whole genome shotgun sequence.
ACCESSION   DATBTE010000234 DATBTE010000000
VERSION     DATBTE010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987296"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..>1864)
                     /locus_tag="VIG33_08595"
     CDS             complement(137..>1864)
                     /locus_tag="VIG33_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8270934.1"
                     /translation="FGLTIVFTMLISLFDAFTVAPMLSAYMASPAEHNKGNGFIARIL
                     KAFDTFQTRLEDLYERFLVKALKYPKSVLAIATFVFVASMMTLPFIPKTFLPTADNGE
                     FSITVELPVGTSLQGTANFTKGIEDILKKDPTIDMVLTTVGSANGEANKASLFVRLVD
                     RKNRQLTTTQTKTVVREKVLPFKEKAIVEVGDIDAVNSGQKPFNLNITGDDLESLSKY
                     ANTLKERLRKIPGLIDVDTNFRSGKPEFHVVFEREKTEHLGVSTAMAGAELRYRTEGA
                     EAAVFRENGIEYKVRVRLEEKYRDLREQFNSTFVPNSNNNMISLNRIAKAEDAFGYSQ
                     INRQNKNRFINLAGNLGPNGSLGGITTEVQRILKEDLPPPPGVDCRFQGQAEDFQDLI
                     SSMLLAIFLGVLFIYLVLSSLYESFVTPFTILLALPLAMCGAMVALLVFGKSIDIFSL
                     IGIVLLLGVVAKNSILLVDYTKQMEQQGFKRNEALLKASRTRLRPILMTSLALIAGMI
                     PIAIGLNEASAMRTSMGIAIIGGLISSTVLTLIVVPAAYGFIDDFRVAAGRFFSRFGG
                     RHHSHEVRE"
BASE COUNT          542 a          471 c          395 g          456 t
ORIGIN      
        1 cagattgaaa gactttaact tcgcccgatc cctacccaag aaaaagaatt tttacagaga
       61 agttcggttg tcattttaga aacaaacaca gtcctgtaag agtctcgata ctagcagtaa
      121 aacactcaac aaataatcac tccctcactt catgagaatg atgacgcccg ccgaagcgac
      181 tgaaaaatct tcctgccgcc acccggaaat catcaataaa tccataggcc gctgggacca
      241 cgatcaaagt cagcacggtc gaactgatca aacccccaat gatggcgata cccatggaag
      301 tccgcatcgc actggcctca ttcagaccga tggcaatcgg aatcatcccg gcgattaaag
      361 ccaaggacgt cataagaatg ggacgaagac gtgtacggct ggcttttagt aaggcttcat
      421 tgcgtttgaa cccctgttgt tccatttgct tggtgtaatc cacgagtaag atagagttct
      481 tcgccaccac acctaacagc agcacaatcc caatgagcga aaagatgtca atggacttac
      541 cgaaaaccaa caaggccacc atggctccac acatcgccag tggcaaagcg agtaaaatcg
      601 taaaaggcgt cacgaagctc tcatacaaag aggacaaaac caagtaaatg aacaagactc
      661 ctaagaagat ggccagcaac atactggaga tcaaatcctg aaaatcctcc gcttgtccct
      721 gaaaacgaca atccactcct gggggcggtg gcaggtcttc tttcaaaatt ctttgcacct
      781 cagtggtgat tccacctaag gagccgttgg gccctaaatt tcccgcaaga ttgatgaaac
      841 gatttttatt ctgacggttg atctgcgagt aaccgaaagc atcttccgct ttggcaatac
      901 gattgaggga aatcatatta ttattggaat ttggcacgaa ggttgaattg aattgctcgc
      961 ggagatcacg atacttttct tccaaacgca cccgcacttt atattcaatg ccattttcac
     1021 ggaagaccgc cgcttccgca ccctcagtgc gataacgaag ctccgctccg gccattgccg
     1081 tggagacacc gagatgctct gtcttctcac gctcaaagac cacgtggaac tccggttttc
     1141 cagaacgaaa atttgtatcc acatcaatca gtccgggaat ctttctcagg cgttctttca
     1201 aggtgttggc atatttactc aaagactcca agtcatcacc tgtgatattt aaattaaagg
     1261 gtttctgacc actgttcacc gcatcgatat ctcccacctc cacgatggct ttttctttaa
     1321 aaggaagaac cttttcacga accacggttt tcgtttgcgt ggtggtgagt tgacggtttt
     1381 tcctatcgac caagcgaaca aacaggctgg ccttattcgc ttctccattg gcggagccca
     1441 ctgtcgtcag caccatatca atggtgggat cctttttaag gatatcttca atgcctttgg
     1501 taaaattggc ggttccttgc aaggaggtcc ctaccggcaa ctccaccgtg atagagaact
     1561 ctccattatc cgctgtgggc aagaaagtct ttggaataaa tggtaaagtc atcatacttg
     1621 caacaaagac aaaagtcgca atcgctaaga ctgacttcgg atattttagt gctttcacca
     1681 gaaagcgttc atagaggtct tccagacgag tttggaaggt atcaaaagct ttgagaattc
     1741 tcgcgatgaa tccattgcct ttgttatgtt ccgcaggcga ggccatatag gccgacaaca
     1801 taggcgccac cgtgaacgca tcaaagagtg aaatcaacat cgtaaagaca atcgtcaaac
     1861 caaa
//
LOCUS       DATBTE010000235        31678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_645134, whole genome shotgun sequence.
ACCESSION   DATBTE010000235 DATBTE010000000
VERSION     DATBTE010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 31678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31678
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645134"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1579
                     /locus_tag="VIG33_08600"
     CDS             167..1579
                     /locus_tag="VIG33_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8270935.1"
                     /translation="MKYRLLQLLLFSLWACAMAACASNGHVAEEYNENLVNRGLHQTS
                     KQTVILFLIDGLPLSTFQEQWAAGNLPQIKKYFIANADKLHVARTTFPALTFPGVGSL
                     LTERTVDQNELYGNKILKEGESLDFESPVNNQKLNDLIRGRNIFARLEKKGLKTVSFD
                     YNFIADSSAHMAPQDAKAGLAILDKKYDYVDSKLIGSLEDLLNQTDPKVWPDFIFIHL
                     IGIDLTSHDQGPKSQTVRKYLTYLDHELRGVFKTLTKIETSKQRKILALMTADHGFDQ
                     KISKSLNLEQTLKYINPQIQVLNEGRYLSLYFPPSWLSERKSSFMQDLASLPMIDIVT
                     YREQNNVFINSDSLELKLAYGPRQCQGNALAFSVTQERAPVQMVGSGPLLCPESLDER
                     LNQLYYPYFLSNLSYYFQSPGHPDAVIIPKPGISFKNESKGQHGGPTPQEVFVPLLMR
                     NCTLRDPQHIPALWELLQFM"
     gene            complement(1594..2268)
                     /locus_tag="VIG33_08605"
     CDS             complement(1594..2268)
                     /locus_tag="VIG33_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY8270936.1"
                     /translation="MSTDYGVSMLKYSFRKYQPLSLRLWHWLNAAVILGLLGTVLLRK
                     TLLSWRTNSTLIQEKLKEAGTIITPELAKDLAVAIRNPLWDWHIYLGFALGALLLGRI
                     AIALFIEKKCPGVHAFKSAMGLKDLPPHEKAEALHYTLVKIGYAFFYLSTALMVVTGF
                     LLNFKTAIGLSKDLVGVTKEIHELMMWFFIFFGVGHVLGVVIAENSKAPGLISDMIHG
                     GDPKIK"
     gene            complement(2281..3033)
                     /gene="gpmA"
                     /locus_tag="VIG33_08610"
     CDS             complement(2281..3033)
                     /gene="gpmA"
                     /locus_tag="VIG33_08610"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEY8270937.1"
                     /translation="MYKMVLIRHGESQWNQENRFTGWQDVDLSEKGRAEAQKGGKALK
                     DKGFSFDVAYTSVLKRAIHTLDFVLNELDQVWLPVHKDWRLNERHYGALQGLNKAETA
                     ARHGEEQVKIWRRSYDTPPPPMEVNDPRHPTKDLRYKNVNPQELPSAESLKDTVARFL
                     PLWKNTIAAEIKADKKVLIVAHGNSLRALMQHLENMSADEIMGVNMPTGIPLMYELDA
                     NLKVIKKEFIGDPEEVRLAMEAVANQGKAAKN"
     gene            3173..5971
                     /locus_tag="VIG33_08615"
     CDS             3173..5971
                     /locus_tag="VIG33_08615"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY8270938.1"
                     /translation="MHKNLMTKTQDFEKTVPSIVKQDEASKGPPVNFASRANLEIIED
                     LYKQFKTNPESVSQDWQHFFQGVDFGQEGSLGLSEKELDVYHLISAYRNYGHFEAQLD
                     PLTNTPAPSDQLSLSRFNLSDKDLEQKFQIGKIIGKPNASLREIIAHLRSCYCGRISV
                     QVAEALPEVRNWFIKEFEASPFKLNNEEKKQTFVSLNRTESLEKFIHTRYVGTKRFSI
                     EGADGMMPMLETLVQRGTQVKVQEVMIGMAHRGRVNVLANFMGKATEYIFADFNGPTE
                     LAVPIDDFDGDVKYHLGYVAQKDTANGKCQVTLAFNPSHLEAVNPVVLGMARAAQRRR
                     NDTQERKTVIPVLIHGDAAFSCQGVNLETFQMSHVKGYTVGGTIHIVIDNQVGFTTNP
                     ENGRSSHYASDTAKVLATPVIHVNGDDAESCVRAMDIALRFRQERGRDVVVNMVCYRR
                     FGHNEGDEPAFTQPLMYEIIKKHPTLREIYGKQLIRENVIDQKFYEDSFQERMDSLQK
                     IYEDTKKTPPKIQPFKFEGFWKGLRKGTGDELLKPVNTKINIKTLKEIGTKLGEYPAS
                     FTPHPKLIKLLESRKNMAAGKEPIDWGMGELLAYGSLLSEGTSVRLTGQDCVRGTFTH
                     RHAGLYDIKTNECYMALDHVNEDKVEFCVYDSILSEYAVLGYEYGNATCDPTFLTLWE
                     AQFGDFANGAQIIIDQFICSAESKWQQMCGLVLLLPHGYEGQGPEHSSAKLERFLQLC
                     AQNNMQVMNLTTPAQIFHALRRQIKRDFRKPMIVMSPKKLLRYPRAVSSLEDLANGGF
                     QEVIPDTVDKSKVDTVVFVSGKLYYDLLEEREKNKKENVALVRLEQIYPFAAEQVAAV
                     LKSYPKLKKLIWSQEEPKNMGAYQNVYFKFTELITQQGLKLEIKYVGRPERSSPATGS
                     IYRHQVEQNEIIQGCFKG"
     gene            5977..7224
                     /gene="odhB"
                     /locus_tag="VIG33_08620"
     CDS             5977..7224
                     /gene="odhB"
                     /locus_tag="VIG33_08620"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEY8270939.1"
                     /translation="MKIDLKIPAVGESISEAVIGSWTKKEGDFVKRNEILMNIETDKA
                     SVEVVAENDGVLHITTPAGQTVAIGATVGYLDTDAKPTSVANTNVSANATAAAAAAAA
                     FVKPTTPPPASSQPASQHLSPAVNRIVTEKGLDPSTLSGTGKEGRLTKADVLAPSPVQ
                     ERSVKTPQPPTVIVPVSRGPSKQGDKKTVPMTTIRKRIAEKLVQAQHTAALLTTFNEI
                     DMTKVNELRAKYKDKFKEKYGVNLGFNGFFVKAAVEALKEFPAVNAFITGNDIEYHNY
                     YNIGIAVSTEKGLMVPNVKDADTLSLAGVEMAIRDLAGKGREGKISIDDLSGGTFSIT
                     NGGVFGSLMSTPILNAPQSAILGLHKVQDRPMAINGKVEIRPMMYVALTYDHRIIDGK
                     EAVSFLVRIKEGVEDPERLLLEI"
     gene            7503..9323
                     /locus_tag="VIG33_08625"
     CDS             7503..9323
                     /locus_tag="VIG33_08625"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY8270940.1"
                     /translation="MRKLLRRRRDKYTEDGLAKSLVLWGGQPSALTLYFAKNAKPTKL
                     EGEALLEDGSKLALPLQIHSETDRGDHWRLQVQWNKTLPLGYHQLTIHTDSRTFKSLL
                     ICAPEKLETAAELKHSWGPFVPLYALRTGSEWGIGSLKEMKLALQKCGEFGAQWLGTL
                     PLLAVNLDPVDCDPSPYSALTRLFWNEIFLDVEALVEKYQHAEARQLIQTAEFQKELS
                     ELRATTHVDYHRVYKLKKRVLKTLAESFFAQNQQNQKAYTDFVADNVEINAYAKFRSP
                     EASEQRFHCFVQFEMNESLREISETLPQGLYLDFPVGVNDAGFDFQEFKPVFFKEVSV
                     GAPPEEVFQQGQEWGFPAFNPYTHQDEGYQYLRKSLRNHLKYCKILRMDHVMGLYRVF
                     TIPKGWTAHDGIYMRFPKNDLFAISMLEAYKAKADIVGENLGTVPAAVDELLHKRNIN
                     GMWVLQLEAWRKPAEAFSLIPQNSMISFNNHDLPMWASFVKGTDLDLVCRLNILRDDS
                     RVKLQAERKNQIEAWSKFTAPENLTLKLHETMAKASGKYFVVTVEDLWGEEAPQNIPG
                     TWKEYPNWRRKYSLPISQWSENKLLQSVFEVLKQHRTRKK"
     gene            complement(9334..10593)
                     /locus_tag="VIG33_08630"
     CDS             complement(9334..10593)
                     /locus_tag="VIG33_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEY8270941.1"
                     /translation="MKKMFVLLFSILGMNSSSFALEADFNAYLRGGTGLNLEGGQQEC
                     FYNIGIPGNFMRLGNECGFYSELAFSFNNKKAEEGNANYFKTQLRLEFNSKGTRQWEP
                     KGDRDINQVEAYVRAGGFSEVPGDFWIGKRFYRDVDLHIFDWYYFADMSGVGAGVENL
                     PVSAGKFSFAHLIQANETLTTSVGRPVLQALDFRWKDLQGLNLWGVYAWAPASSDVAN
                     NYVASSGYSLAVRLQGMVLQGNNNFTLLYGKGTVKDFNIYANSALPATDESPNKAWTF
                     RVVEDWNANLSEKWALMVAAAAEIGDNGTQTKNHREWQEVGVRPTYFVTDRFQWVFEA
                     GYSQIKNDAEVDNNGEPIGVRGLGRISVAPQLSIGKGIWMRPVMRAFLAHSFWTTSNR
                     SFIDDAAPTFKDKNAGTTFGYQFEAWF"
     gene            10758..13412
                     /locus_tag="VIG33_08635"
     CDS             10758..13412
                     /locus_tag="VIG33_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,6-glucosidase domain-containing protein"
                     /protein_id="HEY8270942.1"
                     /translation="MNRVCCLLLIILFQNFPAMAVGVRARWLNSSRILYKLAPGTYIS
                     NSTQFFLMDGDQSVRTGSTIYLPLLSLKDDQALLDTSMLSRTEIKNLISRALKLFIVT
                     NTKQILDSSAIQYAGLLDELYTYEGQDLGGECISDKCNLKLWAPTAQRVRVFLFANAI
                     TPIEQAQILNLSLDGGVWSLSLPIEFKNFYYLYEVQVYQPFTDRVETNLVTDPYSKSL
                     SLNGTRSQLADLNDRGLLPPDWNNLQKPPLNNLKEAVIYELHLRDFSVNDVTIPPELR
                     GTYLAFTEKNSMGMRHLKSLADVGMTHLHLLPFNDFGSVNEDKSSWENYAGKSSNLKE
                     PQSVLSGIRSSDPFNWGYDPVHYMTPEGSYALNGEGTARILEVRQMVKALNESGLRVV
                     QDVVLNHTYENALGAFSVFDKIVPLYYYRLDDHGQAFRTSCCFDTASEHHMMEKLMID
                     TVLHWAKTYKIDGFRFDLMSFHSIATMERLRQALRALTMPKDGVDGSKIILYGEGWSF
                     GSLFNLAPTESMNLENSYGTDYGFFNDRLRDAVRGGTSNSAEKSDQGFATGLFFDFNH
                     EPANRNTPTDEANQRDKLLHLGDVIKAGLAGNLRDFSFKEHLGSRIHAGNLLFRGAPV
                     GVASQALETINFVSAHDGYTLWDAIQAKAPFHTPGRNADLATADERQRIQQLALAIPL
                     LGQGIPFMEAGTDLLRSKNGDQDSYDSGDFFNRIDWTGTTNHWGEGLPPAWKNIDDWA
                     LWHPRLLDPGLRVIPYLIAKTHNYLKALLQVRKSSSLFQLNTLDEISKHLSFIDNETQ
                     PEPGLIAMLLQNQNESLLIFFNASRYGRDFSNRILKNNWAVHPLLNEKVDAVLAEVAI
                     DPNQGTIHLPGRSTVILKLNNISLKGHR"
     gene            13409..15679
                     /locus_tag="VIG33_08640"
     CDS             13409..15679
                     /locus_tag="VIG33_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY8270943.1"
                     /translation="MNFKLNFKMNFGRNFKYITILLSALFIMTFTAPLAHAEKMRIQI
                     WHQMIYGHRQVLAEAIENFEKENPDITVQATYRETEELRSSYQAAAMGGSGPELVYGP
                     SDQIGPFATMGIIQPLDGIYNLDVHPEAFAKFDPLAIPLYQGKHYMLGGLVGNHLMLI
                     YNKKFVSTPPKTTAELIDIGKKLTVDFNGDGKIDQFGLVFNYTEPFFIAPFIPAFGEA
                     LMKDGAQPNLDTEGVLKTFQFILDLRDKYKIIPKECDYETANALFKQNKAAMLINGDW
                     SWGDYKAVNMDFGIARIPMISETGRWPSPLVGTAGYSLNANVKDPERLAATLRVLNYL
                     TSEKTQLLFTERVGVLPSLISLRENPQVKNNPLLQVSSDIMTVGTAMPVVPEIRGVWD
                     SLRIQYQKILAGSVTPEGAAKAAQSMAEAQISNMNEVIQPDASLWVIKIVILLAVLWL
                     LWISRGAGIDFVKGFWGPQRFVYFMMLPAFIGIFAVIVYPFIYNICISLSNFSLRTFQ
                     DWSLIGVHHYVEALSDPKFYTLFFKTLIWTVSNVFFHVTIGVFLAVVIHQVLPAKAFW
                     RTLFIIPWAVPQYITALTWRGMFNQEYGPINLFLQQFLHLSPVQWLSQPFTAFTACIV
                     TNVWLGFPFMMIVALGGLQSIPQSLYEAAYLDKANAWQRFRHITWPLLMPVMVPAALL
                     GCIWTFNNLNIVWLVSNAGEPGDQTHILVSYVYKAAFNLYRYGYAAAVSMLIFLILIL
                     WSLGSLRAQFKKEQKS"
     gene            15676..16506
                     /locus_tag="VIG33_08645"
     CDS             15676..16506
                     /locus_tag="VIG33_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY8270944.1"
                     /translation="MRSLPIQKILAWFSILLFAVFSIYPMLYVLGISLRGDNAFQSQS
                     LEIITSTSTMKNFLDLFTQTDFLIWLRNSLLVSVGTTLLGVAFASTSAYALSRFQFRG
                     RNMMLFSLLATQMFPPTMLILPFYIILSKLHLIDSFWGLFLIYSSTALPFCVWQMKAY
                     YDTIPKELEEAAILDGCSRWLTFCKIILPLSAPALVITALFSFMASWSEYVVAAVVLQ
                     DPQMYTLPLGLRSFQASLATQWGLYAAGALIVSLPVLILFISISRYLISGLTIGSVKG
                     "
     gene            16509..17564
                     /locus_tag="VIG33_08650"
     CDS             16509..17564
                     /locus_tag="VIG33_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8270945.1"
                     /translation="MTHIEFSEITKSFKDNHILNGVNLEIAKGEFLVLVGPSGCGKST
                     LLRTLAGLEKMDAGDIKINGKSIKNMEPQDRDVAMVFQNYALYPHMNVAENMGFAMKL
                     QKRSASEISAQVKKIAQLLQIDHLLERKPKELSGGQCQRVALGRALARQTSIILFDEP
                     LSNLDAHLRSQMRVEIKRLHQTLQNTMIYVTHDQMEATTMGDRIAVLKDGKIEQIGTP
                     SEIYHRPKNLFIASFIGSPEMNFMEGPLVKKLPWPEAKKPEQILGVRPEVFKISNGVP
                     QANELELGSFQVELSENLGGQQMLHGRLEGQNLRIFADSTEFFRKDQILPVKIDLTKS
                     HLFDKQTGVNQRLQEGV"
     gene            17565..18995
                     /locus_tag="VIG33_08655"
     CDS             17565..18995
                     /locus_tag="VIG33_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEY8270946.1"
                     /translation="MPWNGIIQRLRFQKSLLNLAIGALGILLVSLQVHAGPRTAFVHL
                     FEWPWKDVALECENYLGPAGFAAVQVSPPHEHIRTQNSPWWERYQVVSYKLESRSGNE
                     AEFIDMVQRCKKAGVDIYADAILNHMTGMAGGNGLSGTPYSHYDYSPLYSYADFNHCS
                     LNGNDDIQNYSNRYEVQNCELLDLADLATGSEKVRNTLANYLNHLLDLGVTGFRLDAA
                     KHIPAVDLAAIKAKLKRPAYIYQEVIYNPGCPIQYNEYFSVGDVMAYDFPHILARGFQ
                     HKDTNSLLHIADGFPASDSSVIFVTNHDLERGGGVLSFNSSQQPLYRLAQIFMMAWPY
                     GYPQIFSGYQFNDMNAGPPVGSDFRTLSVLDSKSLCKAPWTCEHRLPEVAAMVNFRNQ
                     TDNTFRVDHWWSNGQDLVAFSRGQNGFVAINFSTNPITRTFSTTMSPGTYCNILEGHC
                     NEHFTVENGFVSVIFPPLSAVVLLKN"
     gene            18999..19967
                     /locus_tag="VIG33_08660"
     CDS             18999..19967
                     /locus_tag="VIG33_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEY8270947.1"
                     /translation="MKKMTYTIGLDLGGTKLAAALLNNKGEMLDFIKVPVDMKREASA
                     LRTQKRVISLMTDITMDFKNRFPKETTGPHFKGVGLASAGPLNAAEGKLTYPVNYPGW
                     KIVPIRELLQKDIQRRGFKTKIFFQHDATAAALAEGWVGGAKKMSSYAVVSVGTGVGS
                     GVIFQHLPVQSRGRGSEYGHTIIDYKKLQENPTGLHHCTVEGVASGTGLLRRAKEMGF
                     TGTSVEELVNTKDEKYQILYKDMAWALACLCYNLSIGYNLEKIFLSGGLIKIRSLYFA
                     DLKAHYKSMIQQMNPAFECKIEIAKTKNTAGVLGAGYLPYLSSLKK"
     gene            20068..21225
                     /locus_tag="VIG33_08665"
     CDS             20068..21225
                     /locus_tag="VIG33_08665"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8270948.1"
                     /translation="MKKKVYARILDPFIELKNGSFFLFGPRGVGKSYLLRTVIDQKKT
                     FYINLLDSSLYFRLQRNPSELMDMIISKPVNQVVIDEIQRIPELLNLVHLMIEEQELK
                     FILTGSSARKLKRGQANMLAGRALSRELFPLTWSEIDDFNLKKYLQFGGLPSSYLSAN
                     PDDFLNAYTNTYLKEEIQAEGLVKKLPAFSSFLSMAALSSGEQLNFANISNEVGVSAV
                     TIREYYSILEDTLLGFFVQPWVKSKKRKPISTAKFYFFDTGVRNTLAEIGTLDEKSNT
                     WGQAFEHFIAMELRAFLSYKLKNKIKLCYWRSTSQFEVDFILGDAVAIEVKSAKKVQA
                     NFLKGLKALQEEGLVNKFYLVSNDPLEKVTDGVHCLPWQVFLEKLWSGKII"
     gene            22117..22581
                     /locus_tag="VIG33_08670"
     CDS             22117..22581
                     /locus_tag="VIG33_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270949.1"
                     /translation="MKKESSKKTQNHKFNWEETWFLEKLEYKASMILELHIVGAKKEP
                     PEDLIIDNVNLGQAYPLVWDTSKKVVVRFFQPLAFQRLDESFGAENGGTYTGERFKIY
                     SDSEYLRYYSKVAFGIVDKPIQHYSFTCSDDIIDILSTEGPEIENIQNAPPT"
     gene            complement(22621..23364)
                     /locus_tag="VIG33_08675"
     CDS             complement(22621..23364)
                     /locus_tag="VIG33_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY8270950.1"
                     /translation="MNGIRKSLFLSLILSFTPVSYSQSETSTHKEASHAAADSGVNAK
                     TSFSWLKNGNSRYVKGFLRKDGQSNRDRQRLVGGQKPHAIVLSCSDSRVPPEVLFDQK
                     LGEIFVVRTAGEALDNAAIASVEYALEHIGSHLLVVMAHESCGAVKAAFSTLKGEDAG
                     SPALNALVKDIHPRISKFSGKEPSQGYISEARANASGVARDLVARSKIVSEKLKSNDL
                     VIETAVYHLGSGLAEFYTEKADRGLASDK"
     gene            23639..24220
                     /locus_tag="VIG33_08680"
     CDS             23639..24220
                     /locus_tag="VIG33_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270951.1"
                     /translation="MKNGFIALVLIMSASWACAENLHPFHSDGCSGSPDSILGTSYIH
                     CCIAHDFEYWVGGTKTQKNQADENLKQCIQRTIPSLKMVGSIYKLGVIIGGTPTFFGA
                     INSPFTWRWGYGWEEDHGYSPSTKQELELALIDLQRMEADFDAHERALNETQGFAWRF
                     GNLKISPEQFKQIKKDLQKKINSIISILNDTDL"
     gene            24243..24944
                     /locus_tag="VIG33_08685"
     CDS             24243..24944
                     /locus_tag="VIG33_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270952.1"
                     /translation="MLISIYGLTHSQYQNIAIPIAKSFSAKFSSDFETRVIYQATQED
                     LYSALNNPQSVGVFWLSHSNSGIQASGIGMGGSIVDSKGVDVSRVFTKIHPNIKWIGV
                     IGCKALPIFKGFADKGFYKDKNIFLHDQAFNWNALVGMDHSTSLTSFSGLVISSSTKL
                     ALTNIDSDRNFKNATTYLQRLKRENQLTDVQTPADSNVLRFKVKRVPDHFNKNEMVAV
                     QVLVNSLILLTDPES"
     gene            complement(25025..26218)
                     /locus_tag="VIG33_08690"
     CDS             complement(25025..26218)
                     /locus_tag="VIG33_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEY8270953.1"
                     /translation="MKMGVEVLLEDKKLIQVLKKKRVALICHPASVDEKLRHSFDLLH
                     KTIGLSAAFGPQHGVKGEKQDNMIESEDFIHPDYKIPIYSLYGKVRTPSPKMMETFDV
                     AIFDLQDLGCRIYTFITTLLYVMQECARRKKQVIILDRPNPAGREVEGFSLRPGWESF
                     VGAAECPMRHGLTCGEMALFYKEHFKLDLDLKVIKMKGYHCEKKPGFGWPMDRAWVNP
                     SPNAQTLNMARAYCGTVMIEGTTLSEGRGTTRALEQVGASDIDFTEVLGRMQKKAPQW
                     LKGIILRECFFEPTFHKHEKKLCHGFQIHADIAAYQPQLFKPYRLIALMLKCIREIHP
                     NYALYRDFAYEYTVGKLAFDVINGGPELREWIENPKATPADLEKALKKDEKAWLKTRK
                     KFLLY"
     gene            complement(26215..26793)
                     /locus_tag="VIG33_08695"
     CDS             complement(26215..26793)
                     /locus_tag="VIG33_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270954.1"
                     /translation="MPNLISFSPPFSDPNAMEPLCSGETLLLEADLQFKDSSLYLEFG
                     FLEQSAELFELPKTSASWPEAQVPRQDGLWQATCFEAFLSPLGSSKYFEFNFSLNPAW
                     NAYKFESYRLPQPAAPTEEFVLKSMGWDAVKKRLLVELENKTFYSQFRVGLTAILLEK
                     NGTKHYCALAHKGTQPDFHLMKSFILQRGTEE"
     gene            26924..28336
                     /gene="lpdA"
                     /locus_tag="VIG33_08700"
     CDS             26924..28336
                     /gene="lpdA"
                     /locus_tag="VIG33_08700"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015466955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY8270955.1"
                     /translation="MSESTQFDLVIIGSGPGGYVGAIRASQLGLKTAVVEKESTFGGT
                     CVRVGCIPSKALLESTEHFEAAQHDFASHGIKVSKVELDLTTMMSRKDKVVKSNTDGI
                     SFLFKKNKITSFQGTGKILKAGSVEVTAADGSKQVILTKNIVIATGSVPVELPFLKYD
                     EKKIVSNTGALTLLEVPKTMIVVGGGVIGLELGSVWGRLGSQVTVIEYADRLGGPMDQ
                     DCMNVLKKNMEKAGVKFLLSTKVTGSKVTGKGVEITYEALADGKAQSMTADVVLVSTG
                     RKAFTAGVGCEEVGIQKDPQGRIIIDKHYQTNVPGIYAIGDVVAGPMLAHKAEEEGVA
                     LAELLSGHAGHVNYETVPGVIYTHPEISSVGMTEEQAKAQGLEINVGKFPFMANGRAR
                     AKGFTEGFVKIIADKTTDRILGCHMVGPGVSELIHEVIVCMEFGGSSEDLARSFHAHP
                     TLSEVVREAALAVEKRQRQM"
     gene            complement(28278..29834)
                     /locus_tag="VIG33_08705"
     CDS             complement(28278..29834)
                     /locus_tag="VIG33_08705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8270956.1"
                     /translation="MMWKSKIDGTLEYVSKSWTSYTGLSKEQSIGLGWQKAIHISDLS
                     GFLRSWNESMKSGESFQIECRVRRHDNQMRWHWLLVVPELNTLDMRVGWIGTCADIHD
                     RKLAERKLVEAERMAVSANIVKTHFLANMSHEIRTPLSAILGFAELMLSPHLSFEEQT
                     HYVHTIHRSGHQLLKIIDQILDISKVESGHMEIENIDVNLGDLLRDLRDLLMVKAKSK
                     GVGLEFRFKSLVPEHIFSDPTRVRQILMNVIGNAIKFTEKGKVVVEAEWIEATLLRPS
                     VLAFRVQDTGVGIEESHTQNLFQPFVQVDSSNTRRFGGTGLGLALSRKIAQALGGDVL
                     LEKSERGQGSVFVIEIETDTLLDSKFTDKVEDFIVKDLESPAADEAHALSGKKVLLVE
                     DAPINQLLISRFLTGAGAQVEVAENGVLGVQKALAGDYAVVLMDIQMPEMDGYEATAK
                     LREQDYKRPIIALTAHALKEDQERCLQAGCSDYLTKPIDRKLLISRILHFTSDVGVFP
                     PLRPPPELLR"
     gene            30053..31378
                     /locus_tag="VIG33_08710"
     CDS             30053..31378
                     /locus_tag="VIG33_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270957.1"
                     /translation="MSLVKFSFLFLMTFAFWTPQADAEVWTTVNTWDAQWEKNYTEWV
                     EKTWSADFFSRASLPNGQNNPYYGVRADCADTVYSMRLIYSFENKLPFAFQDPTSTGA
                     LISNSMNRWNRYSDELKRFRSFLAYVMGIVSTTSLPNDTFPVPVSRDWIHSGGLIRTT
                     KVNHHSWTIREIQAIGVPHLIFNSTVGRQSGFSLQERVSWPNPKWVFEGDFSAASNAG
                     FRYWRPLEFINRPVWETPGYSEEQFSIPLGKWQKTLQNKLALHQETSIQMLRRLSLTA
                     CNALKDRITAVNDGLTFLRSLPSNQCMSFENYDTYSTPNRDQRLFDDMAALRRSYKDI
                     LHGKNSLPEDMKIQLNKVFPLIQRSAQKETSVMQVSNMDTASWCPVEFSPGEKIDFAE
                     AKRRLFLGQMSNNPLEEVEYRWGTVQGPSARAQACESWDIWKPDLKLAD"
     gene            31385..>31678
                     /locus_tag="VIG33_08715"
     CDS             31385..>31678
                     /locus_tag="VIG33_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270958.1"
                     /translation="MERKRRTRWSLIIVSLFFISLAVDYFFLHLLFRQRLKEQQQQKE
                     QQEEMTPLREQSQSLILKPNVSSAGTEDNRKLRSPLQEEFQDKLKKCLGPKLGK"
BASE COUNT         8708 a         7498 c         6997 g         8475 t
ORIGIN      
        1 cctcaagtct gctcaaaccc ctatccccac aaaaagaaga aagaggcaag agtccgtcga
       61 tcttatgaac catagcgtaa ttcttatatt ctctttcttg ctaaactcaa ttgtttgtct
      121 gtagcttcgc tgtggcctgg ccgctgaatt gcttattgag ctctctgtga aataccgctt
      181 actgcaactc ttacttttca gtctctgggc ctgcgcgatg gctgcttgtg cctcgaacgg
      241 tcatgtggct gaggaataca atgagaattt agtgaatcgc ggcctccacc agacttcgaa
      301 gcaaacggtc attttatttt taattgatgg cctaccactt tcgacttttc aagaacaatg
      361 ggccgcgggg aatttgccac aaatcaagaa atactttatt gcaaacgccg ataaactgca
      421 tgtggcacgt accacatttc cggctctgac ttttcccggg gtgggatctc tgcttacaga
      481 gaggaccgta gatcagaatg agctttacgg caataaaata ttgaaagagg gggaatcttt
      541 ggattttgaa tctcccgtga ataatcagaa actaaatgac ctgatccggg gtcgtaatat
      601 attcgcccgg cttgagaaaa aaggtcttaa gaccgtaagt tttgattata attttatcgc
      661 cgattccagc gctcacatgg ctccccaaga tgcaaaggcg ggccttgcga tccttgacaa
      721 aaaatatgat tacgtggatt caaaacttat tggctcccta gaggaccttt tgaatcaaac
      781 agatccaaag gtctggcctg attttatttt tattcatttg atcgggattg atttaaccag
      841 tcatgatcag ggacctaaat cccagacggt taggaaatac ttaacgtatc tggatcatga
      901 attgagaggc gttttcaaaa ctctgactaa aatcgaaact tcaaagcaaa gaaaaattct
      961 tgcgcttatg actgcggatc atggcttcga ccagaaaatt tcaaagtccc tgaatctgga
     1021 gcagacttta aaatatatca atccccaaat ccaagtgctc aatgaaggac gttatctcag
     1081 tctttatttt ccaccgagtt ggctgagcga gcgaaagtct tccttcatgc aggatttggc
     1141 ctctctgcca atgattgata ttgtgactta tcgggaacaa aacaatgttt ttataaactc
     1201 tgactcctta gaattgaagc ttgcttatgg cccccgccaa tgccaaggca atgccttggc
     1261 attttcagtc acccaggaaa gagctcctgt gcagatggta ggcagtgggc ctttgctttg
     1321 tccggagagc ttggatgaaa gactcaatca actttactat ccctattttt taagtaatct
     1381 ctcttactat tttcaaagcc caggtcaccc cgacgccgtg attattccaa aaccggggat
     1441 ttcctttaaa aatgaaagca aaggtcaaca cggaggtccc actccccaag aagttttcgt
     1501 cccacttctt atgaggaatt gtactttgag ggaccctcaa cacattcccg ctctctggga
     1561 gcttttgcag ttcatgtaat tgactgctgc tcgctattta atcttgggat cgcccccatg
     1621 gatcatatcc gagataagcc ctggggcttt gctattttcc gcaataacga ctcctaaaac
     1681 atgccccaca ccaaagaaaa taaagaacca catcatcagt tcatggatct ctttggtcac
     1741 acccacaaga tccttcgaca agcctatcgc cgttttgaaa ttgagtagga accccgtaac
     1801 caccatcaga gcggtggaca aataaaagaa cgcatagcca atttttacca atgtataatg
     1861 aagggcttct gccttttcgt gaggcggaag atcttttaaa cccatagcac ttttgaaggc
     1921 atgaaccccg gggcatttct tttcaataaa gagggctatt gcaatacgcc caagcaaaag
     1981 agcccccaag gcaaaaccca aatagatatg ccagtcccac aagggattgc gtattgcgac
     2041 ggcaaggtcc ttagccaact caggagtaat gatcgtgccc gcttctttca acttctcttg
     2101 aattaaggtt gagtttgtac gccagcttaa aagtgttttt ctaagcagaa ctgttcctaa
     2161 cagacccaag atcaccgctg cattcaacca gtgccaaaga cgaagactta aaggttggta
     2221 ctttcgaaaa ctatatttca acatcgaaac tccgtagtca gtggacatat aacttctccc
     2281 ctaatttttt gcggccttcc cttgattcgc aacagcttcc atcgccagac gcacttcttc
     2341 cggatcccca atgaattctt tttttatgac cttgagattg gcatccagct catacatcag
     2401 agggatgcct gtgggcatat tgacgcccat gatttcatcc gctgacatgt tttccagatg
     2461 ttgcatcaga gctcgcaaag aatttccatg ggccacgatc aaaacttttt tatcggcttt
     2521 aatctcagcc gcaatggtgt tcttccacaa agggagaaag cgcgccacgg tgtctttcaa
     2581 agactccgca ctcggaagct cctgagggtt cacattctta tagcgaaggt ccttggtggg
     2641 atggcgcggg tcattcacct ccatcggtgg aggtggagtg tcatagctcc ggcgccagat
     2701 cttcacctgc tcctccccat gacgggctgc ggtttctgct ttgttcaatc cctgaagggc
     2761 gccgtagtgg cgttcattca agcgccaatc tttatgcaca ggaagccaca cttgatccaa
     2821 ttcattgagc acaaaatcga gagtatggat ggcccgcttc aggacgctgg tgtaggccac
     2881 atcaaaacta aaacctttgt ctttgagagc tttgccgcct ttttgagctt cggctcgacc
     2941 tttctctgaa agatctacgt cctgccagcc cgtaaagcga ttttcttgat tccattgact
     3001 ctcaccgtgg cgaataagca ccattttgta catcttgatt gggtctccag gctttgcttt
     3061 agtcctcttt ggactgatat taggttttcc tttttgaaaa aagggattat catgagtggg
     3121 tcgggtctcc gtcaaaagct ttgcattttg ccgcgcataa aaatgtacaa aaatgcacaa
     3181 aaatctaatg acgaagactc aagactttga aaaaacagta ccctcaattg tcaaacaaga
     3241 cgaagcatca aaaggacctc ctgtgaattt tgcaagtcgc gctaatttag aaattattga
     3301 agatctctat aaacagttca aaaccaatcc cgaaagcgtc agccaagatt ggcagcactt
     3361 cttccaaggg gtagacttcg gtcaagaagg cagtctgggc ctttctgaaa aagaactcga
     3421 tgtttatcat ctgatttcgg cttaccgtaa ctacggccac tttgaagctc aattggatcc
     3481 cctcaccaac acccccgctc cttcagacca gttgtctctg agcagattca atctttcaga
     3541 caaagatttg gaacaaaaat ttcaaattgg aaagatcatc ggcaaaccga atgcttctct
     3601 gcgggagata atcgcccatc tgcgttcttg ttactgtggt cgcatcagcg tccaagtggc
     3661 tgaggccctg ccggaggttc gtaactggtt catcaaagaa ttcgaagctt ccccgtttaa
     3721 gttgaataac gaagagaaaa aacaaacctt cgtctcttta aatcgaactg agtctttaga
     3781 aaaattcatt cacactcgtt acgtgggcac caaacgtttc tctatcgagg gcgccgacgg
     3841 catgatgcct atgttggaaa ccctggtcca gcgcggcacg caagtgaaag tgcaagaagt
     3901 gatgatcggc atggcccatc ggggtcgcgt gaatgtgctc gccaacttca tgggtaaagc
     3961 caccgagtat attttcgcgg atttcaatgg ccctacagag cttgcggttc ctattgatga
     4021 tttcgatggc gatgtgaaat atcatttggg ctatgtcgcg caaaaagaca ccgccaacgg
     4081 caaatgccaa gtgactttgg ccttcaaccc ttctcatctc gaagcggtca atcctgtggt
     4141 tttggggatg gcgcgggcgg ctcaacgtcg cagaaatgac actcaagagc gtaaaaccgt
     4201 gattccagtt ttgattcacg gagatgcggc tttttcttgc caaggggtta acttagaaac
     4261 tttccaaatg tcccacgtga aaggttacac cgtgggaggc accatccaca ttgtgatcga
     4321 caatcaagtg ggctttacca ccaatcctga aaacggccgt tcttcccact atgcatccga
     4381 taccgccaaa gtcttggcga cgccggtgat ccatgtgaat ggcgatgatg ctgaaagctg
     4441 tgtgcgcgcc atggacattg ctctgcgctt ccgtcaagag cggggccgcg atgtggtggt
     4501 gaatatggtt tgttaccgcc gctttggaca caatgaagga gacgaacccg ccttcaccca
     4561 acctttaatg tacgaaatta ttaaaaagca cccgactctg cgcgagattt atggcaaaca
     4621 attgatccgg gaaaatgtga ttgatcagaa attctatgag gactctttcc aagagcgcat
     4681 ggattctctg caaaaaattt acgaagacac gaaaaagaca ccgccaaaaa tccagccttt
     4741 caaatttgag ggcttctgga aaggtcttcg caaagggacc ggcgatgagc tcttgaaacc
     4801 agtgaacacg aaaatcaaca tcaagactct gaaagagatc gggacgaagc tgggagaata
     4861 ccctgcgagc ttcactcccc atccaaaatt aattaaactg ctggagtccc gtaaaaatat
     4921 ggctgctgga aaagagccga tcgattgggg catgggcgag cttctggctt acggctctct
     4981 cttgagtgag ggaacctccg tgcgcttgac cggtcaagat tgtgtgcgcg gtaccttcac
     5041 ccatcgtcac gcgggccttt atgacataaa aaccaacgaa tgctatatgg ccttggatca
     5101 cgtcaacgaa gataaagttg aattctgtgt ctatgattcc attttgtcag agtatgcggt
     5161 tttaggttat gagtatggca atgcaacctg cgacccgact ttcctgactt tgtgggaagc
     5221 acaattcggg gacttcgcca atggagccca gatcatcatc gatcagttta tttgtagtgc
     5281 ggaatctaaa tggcagcaaa tgtgcggcct ggttctgctt ctgccccatg gctatgaagg
     5341 tcagggaccc gagcattcct ctgcaaaact agagcgattc ttgcaactgt gcgctcagaa
     5401 caatatgcag gtgatgaatc tgacgacccc agcacaaatc ttccatgcgc ttcgtcgcca
     5461 aatcaaacgc gatttccgca aacctatgat cgtgatgagt ccgaagaaat tacttcgtta
     5521 tccccgcgcg gtgtctagct tagaagactt ggcgaatggc ggattccagg aagtcattcc
     5581 tgacactgtc gataagtcga aagtggatac cgtggttttc gtcagtggca aactgtacta
     5641 cgatctgctg gaagaaagag aaaagaacaa aaaagagaat gtggctttag tccgtttgga
     5701 acagatctat ccgttcgcgg cggaacaagt ggcggcggtc ttgaaatctt atccgaaatt
     5761 gaagaagttg atctggtctc aagaggagcc taaaaacatg ggcgcttacc agaatgtcta
     5821 tttcaaattc acggaattga taacacagca aggtctaaag cttgaaatca aatatgtagg
     5881 ccgtccagaa cgttcctcac cagcgactgg ctcgatctac agacaccaag ttgaacaaaa
     5941 tgaaattatt caaggatgct ttaaggggta actactatga aaatcgattt aaaaattccc
     6001 gcagttggag agtctatcag cgaagcggtg attggcagct ggactaaaaa agaaggcgac
     6061 ttcgtcaagc gcaacgaaat cttgatgaat atcgaaacgg ataaggccag cgtggaagta
     6121 gttgccgaga acgacggcgt tcttcacatc accactccgg caggacaaac cgtagcgatt
     6181 ggtgccactg tgggctacct cgatacggac gccaaaccaa cgtcagttgc taacaccaac
     6241 gtcagtgcca atgcaacggc agcggcggcg gctgcagcgg cttttgtaaa accaacaaca
     6301 cctccgccag catcctcaca acctgcgtct caacatcttt ctcctgcggt gaacagaatc
     6361 gtcacggaaa aaggtttgga tccttcgact ctctcaggaa ctggcaaaga gggacgactt
     6421 acaaaagcag acgtgctcgc cccttcacct gtccaggaac ggtcggtaaa aactcctcag
     6481 ccccccactg tgattgtacc ggtgtcccga ggtccatcaa aacagggcga taaaaaaacc
     6541 gttcctatga ccaccatccg taaacgcatt gctgaaaaat tagtgcaagc tcagcacaca
     6601 gcggctcttt taaccacttt caacgaaatc gacatgacaa aggtcaacga gcttcgtgcg
     6661 aagtacaaag ataagttcaa agaaaaatac ggcgtgaatc tgggctttaa tggcttcttc
     6721 gtgaaagccg ctgttgaggc tctcaaagaa tttccagcgg tgaatgcctt tatcaccgga
     6781 aacgatattg agtaccacaa ttactacaat atcggcatcg cggtctccac agaaaagggt
     6841 ctgatggttc ctaatgttaa ggatgcggac accctgtcat tggcaggggt tgagatggcc
     6901 atccgtgatc tggcgggcaa aggccgtgaa ggaaaaatct ccattgacga tttgagcggt
     6961 ggaactttct ccatcaccaa tggcggagtt ttcggttctt tgatgtcgac tccgatcttg
     7021 aacgctcctc aaagcgcgat cttaggtctg cataaagtcc aagaccgccc catggcgatc
     7081 aacggcaaag tagaaattcg cccgatgatg tacgtggcgc ttacctacga ccacagaatc
     7141 atcgacggca aagaagccgt cagcttccta gtaagaatta aagaaggcgt cgaagaccca
     7201 gaaagacttc tcctggagat ctagaaggta cggcgtacct tctagtagtg cgccgcaaaa
     7261 agcctggttg ggagatcagg cttttttgtt aattcttgca ccgaaagccc ttgccgacta
     7321 gtcttcgcgt catgactttt aataaaatac ttcttcgtaa tacggctaga gcatgggggc
     7381 tacaggcttc ttatctggtt aatggacaaa ataatattca gagtgctatc tgggctccgg
     7441 aaaaatctct attaaaagtt ctaagtgatc tcactcaaac aaagatttac tcggatgaag
     7501 atttgagaaa actcttacga cgccgccgtg acaaatacac tgaagatgga ctggcaaaat
     7561 ccctggtact ttggggcggc caaccttctg cactgacgct ctattttgcg aagaatgcta
     7621 agcccacgaa acttgaaggc gaagcactgc tggaggatgg cagtaagctt gcgctgcctc
     7681 tgcaaatcca ctcggaaacg gaccggggcg atcattggcg actgcaagtg caatggaaca
     7741 aaactttgcc cttgggttac catcaattga cgatccacac agactcgcga acctttaaaa
     7801 gtcttctcat ctgtgctccg gaaaaactgg agactgccgc ggagttaaag cattcctggg
     7861 gcccttttgt acctctctat gctcttcgca ctgggagtga gtggggcatc ggctctctca
     7921 aagaaatgaa acttgcactg cagaagtgcg gagaatttgg cgctcaatgg cttggcactt
     7981 tgcctttatt ggcggtgaat ctggatcccg tggattgtga tcccagtcct tactccgctc
     8041 tgacccgtct attctggaac gagatctttt tagatgtcga agccttggtc gaaaaatatc
     8101 agcacgcgga agctcgccaa cttattcaga ccgctgaatt tcaaaaagaa ctcagcgaac
     8161 tgcgcgccac gacccatgtg gactaccatc gtgtttacaa attgaaaaag cgcgttttaa
     8221 aaacgcttgc tgaaagtttc tttgcgcaaa atcagcaaaa tcaaaaggcc tatacggatt
     8281 tcgttgcaga caatgtcgag atcaatgcct atgcgaaatt tcgttctccg gaggcaagtg
     8341 aacagcgctt ccattgcttc gtgcaatttg aaatgaatga atccctacga gaaatttcag
     8401 agacgctgcc ccagggtctt tatcttgatt tcccggtggg cgtgaacgat gcgggttttg
     8461 atttccaaga attcaaaccg gttttcttta aagaagtttc cgtgggagca ccgccggagg
     8521 aagtttttca gcaaggacaa gaatggggct tccccgcttt caatccctac acgcatcaag
     8581 atgagggcta tcaatatttg cgcaagagtc ttcgtaatca tttaaagtac tgtaaaattc
     8641 tacgcatgga tcatgtgatg ggactttatc gtgtctttac tattcctaag ggctggaccg
     8701 cccatgacgg aatttatatg cgttttccca agaatgatct tttcgccatt tccatgttag
     8761 aggcctacaa agccaaagcc gatatcgtcg gagaaaattt aggcactgtt cccgccgcag
     8821 tagatgagct tttgcataag agaaatatca acggcatgtg ggttctgcaa ctcgaagcct
     8881 ggagaaaacc cgcagaggct ttttcactga ttccacaaaa ttccatgatc agcttcaaca
     8941 atcatgatct tccgatgtgg gctagtttcg tgaaaggaac agatctggat ctggtgtgcc
     9001 gtctgaatat tctccgcgac gattcgcgag tgaaactcca agccgaacga aaaaatcaga
     9061 tagaagcttg gtccaaattc accgccccgg aaaacctaac cctgaagctg catgaaacca
     9121 tggccaaagc ttccggaaag tactttgtcg tcaccgtgga agatctgtgg ggcgaagaag
     9181 ctccacaaaa tattcctggg acttggaaag agtatccgaa ctggcgtcga aaatactccc
     9241 tgcccatttc acagtggagt gaaaacaaat tgcttcagtc cgtctttgaa gttcttaaac
     9301 agcaccgcac tagaaaaaaa taaactcaaa aatctagaac caagcctcaa actgataacc
     9361 gaaagtggtg ccggcatttt tatctttaaa tgtgggcgcg gcatcgtcaa tgaaactgcg
     9421 gttcgaagtt gtccagaaag aatgcgccag gaaggctctc atcacagggc gcatccatat
     9481 tccttttcca atggataatt ggggcgccac gctgatccga ccgagtcccc tgactccgat
     9541 aggttcgccg ttgttatcca cttcagcatc gtttttaatt tgcgagtaac ctgcttcaaa
     9601 aacccattga aatctatccg tcacaaagta ggtgggtcgc actcccactt cttgccactc
     9661 gcgatgattt ttagtctgcg tgccattgtc accaatttcg gcggcggccg ccaccatgag
     9721 tgcccacttc tcgctcagat ttgcattcca gtcctcaacc actcggaaag tccaagcttt
     9781 gttcggactc tcatccgtcg caggcagggc actgttcgca taaatattaa aatccttcac
     9841 ggtgcctttt ccataaagca gagtgaaatt attgttaccc tgtaagacca ttccttgcag
     9901 acgtaccgca agagagtaac ccgaactcgc cacataattg tttgcaacat ctgaacttgc
     9961 cggcgcccaa gcataaactc cccacaaatt caacccctga agatccttcc aacgaaaatc
    10021 caaggcttga aggacgggcc ttcccacgga agtggtgaga gtttcattgg cttgaatgag
    10081 atgggcgaaa gaaaatttac ccgcacttac agggagattc tccacccctg cgccgacgcc
    10141 actcatgtct gcaaaataat accaatcgaa aatatgaaga tcgacatcac gatagaaacg
    10201 ctttcctatc cagaagtctc cgggaacttc agaaaatcca ccagcacgga cgtaggcttc
    10261 gacttgattt atgtcacggt caccttttgg ctcccattga cgcgtgccct tcgaattaaa
    10321 ttctaggcgc agctgcgtct taaaataatt cgcattgcct tcttccgctt ttttattgtt
    10381 aaaagaaaaa gcaagttctg aataaaatcc gcattcattc cctaaacgca tgaaattacc
    10441 agggattcca atgttataaa aacattcttg ctggccgccc tcgagattga gcccagtgcc
    10501 tccacgaaga tacgcgttga aatccgcctc tagagcaaag ctcgacgaat tcattcctaa
    10561 aatggaaaac aaaagtacaa acattttttt cattttttct ccgcgctatt ctcgctcttc
    10621 attcttatat tcagctctgc taatccgttg caatattccc caaaaaaata agtctcttga
    10681 aaaatgtaag ggatactcat aaagtcttcg cctggccaca ctttatgcaa acggtcttac
    10741 attttaggga ggggattttg aaccgagtct gttgcctgct tttaattatt ctatttcaaa
    10801 attttcccgc gatggccgta ggcgtacgag cgcggtggct gaactcctca agaattctat
    10861 acaaactggc tcctggaact tacatcagca atagtacgca attttttttg atggatggag
    10921 accaatccgt cagaacaggc tccaccattt acctgccttt gctttctctg aaagatgatc
    10981 aagctctgct agatacttcc atgctttcca gaacggagat taaaaatctc atctctcggg
    11041 cgctgaaact ctttatcgtt accaacacta aacagatctt agattcatcc gcgattcagt
    11101 acgcagggct cctggatgaa ctgtatacct atgaaggtca ggatcttggc ggagagtgca
    11161 tcagtgacaa atgcaatctc aaactctggg ctcccaccgc tcaacgcgtt cgtgttttcc
    11221 ttttcgccaa tgccataact cccattgagc aagcgcaaat tcttaacctg agtttagatg
    11281 gcggggtctg gagcctttca ctgccgattg aattcaaaaa cttttattat ctttatgaag
    11341 tgcaggtcta ccaacccttt acggatcgcg tagaaacaaa tctggtcact gatccttata
    11401 gcaaaagttt aagtctcaac ggcactcgct cccaattggc agacctcaac gatcgcggcc
    11461 tactgccgcc ggattggaat aatctgcaaa agccgcccct gaataacctt aaagaagctg
    11521 tgatctatga acttcatctc cgtgacttca gcgtcaacga tgtcacaatt cctccagaac
    11581 tgcgcgggac ctatctggct tttactgaaa aaaactccat gggaatgcgc cacttaaaat
    11641 ccctggcaga tgtggggatg acccatctgc atctgctgcc cttcaatgat tttggcagtg
    11701 tgaacgaaga taaatcaagc tgggaaaact atgcgggcaa aagctccaac ttgaaagagc
    11761 cgcaaagtgt cctgagcggc attcgttcca gtgatccttt caactggggc tatgatcccg
    11821 ttcactatat gacccccgag ggcagttacg cccttaacgg agagggcacc gcacgaatcc
    11881 tcgaagtccg ccaaatggtc aaggccctga atgaatccgg cttgcgcgtg gtgcaagatg
    11941 tggtactcaa tcatacttac gaaaatgctc tgggagcttt ttcagtgttt gataaaatcg
    12001 tgcctcttta ttattaccgt ctcgatgatc acggccaggc ttttcggacc tcgtgctgct
    12061 ttgataccgc gagcgagcat cacatgatgg aaaagctgat gatcgatacg gttcttcatt
    12121 gggctaagac ctacaaaatc gatggctttc gttttgatct gatgtctttt cactccatcg
    12181 ccacgatgga gcgtcttcgg caagctttgc gtgctttaac gatgccaaaa gatggagtgg
    12241 atggttctaa aattattctc tatggagagg gttggagttt tggctccctc tttaatcttg
    12301 cacccactga atccatgaat ctcgaaaact cctatgggac tgattatggt tttttcaacg
    12361 atcggctgcg cgacgcggtt cgcgggggaa ccagcaattc cgccgaaaaa tctgatcaag
    12421 gctttgccac cggtttattc tttgacttca atcatgaacc tgccaatcgc aacacaccca
    12481 ctgatgaggc caaccaaagg gacaagcttt tacatttagg cgatgtgatc aaagcggggc
    12541 tagccggaaa tttgcgcgat ttctctttca aagaacacct gggttcgagg atccatgccg
    12601 ggaatttact cttccgcggg gctccggtcg gagtggcttc gcaagcgctg gaaactatta
    12661 attttgtctc agcccacgat ggttatactc tttgggatgc gattcaagct aaagctcctt
    12721 tccatactcc cgggcgcaat gcggatctgg ccactgcgga tgagcgtcag cgcattcaac
    12781 agctcgcctt ggcaattcca cttttgggcc aaggcattcc ctttatggaa gccggcaccg
    12841 atctgttacg ttccaaaaac ggagaccaag acagctatga ttcaggggac tttttcaatc
    12901 gtattgattg gaccggcact accaatcatt ggggagaagg tcttccaccc gcttggaaaa
    12961 atattgatga ttgggcttta tggcatcctc gcctcttaga ccccggtttg agagtcattc
    13021 cttatctgat cgcgaagact cataattatc tgaaggcttt gcttcaggtg agaaaaagtt
    13081 caagcttatt tcaactcaac accttggatg aaatttcaaa acatctttct tttatcgaca
    13141 atgagactca gccagagcca ggcttgattg cgatgctttt gcagaatcaa aatgaatctc
    13201 ttttgatttt tttcaacgcc tctcgctatg gccgcgactt ctcaaatagg attttaaaaa
    13261 ataactgggc agttcatcca ctcttgaatg aaaaagtcga tgctgtttta gctgaggtgg
    13321 ctatagatcc aaatcagggc accattcacc ttcctggtcg cagcacggtt attttgaaat
    13381 taaataatat ttcactcaaa ggccaccgat gaactttaag ttgaacttta agatgaactt
    13441 tgggcggaac tttaaatata taacaattct tttgtcggcc ctttttatca tgaccttcac
    13501 ggcccctctc gctcatgctg aaaagatgcg aattcagatc tggcatcaga tgatttatgg
    13561 acatcgccaa gtcctggctg aagccattga aaattttgaa aaagaaaatc cagacatcac
    13621 cgttcaagcc acttatcgtg agactgaaga acttcgctca agttatcaag ccgcagccat
    13681 gggtggcagc ggtcccgagc tggtatatgg tcctagcgac caaatcggtc cttttgccac
    13741 tatggggatc atccaaccgc tcgatggaat atataatctc gacgtccatc cagaggcctt
    13801 cgccaaattt gatcccctcg ccatcccgct ttatcaaggc aaacattata tgctgggcgg
    13861 attggtggga aaccacctta tgctcatcta taataagaaa tttgtctcta ctccacccaa
    13921 aaccacggcg gagctgattg acatcggcaa aaaattaacc gtggatttta atggggatgg
    13981 aaagatcgac caattcggct tggtctttaa ctataccgag ccttttttta ttgctccctt
    14041 tattcccgct tttggcgaag cgctgatgaa agacggcgcg caaccaaact tggacacgga
    14101 aggagtgctt aagacttttc agtttatttt agatcttcgt gacaaatata aaatcattcc
    14161 taaagagtgc gattatgaaa ccgccaatgc tctgtttaag caaaacaaag cggctatgtt
    14221 aatcaacgga gactggtcct ggggggacta taaagcggtc aacatggatt tcggcattgc
    14281 ccgcattccc atgatttccg agaccggtcg ctggccaagt cctttggtgg gtacggcggg
    14341 ttattccttg aacgcgaatg tcaaagatcc agagcgcctg gctgccacct tgagagtctt
    14401 gaattatttg acctctgaaa aaactcaact tctcttcaca gaacgcgtgg gagttttgcc
    14461 ttctcttatc tcgctgcgcg aaaatccaca ggtgaaaaac aatcctttgc tgcaagtgtc
    14521 ttctgatatc atgaccgtgg gaacagcgat gcccgtggtc cctgaaatcc ggggggtgtg
    14581 ggatagcttg cgcattcagt accagaaaat tcttgcgggc tctgtgactc cagaaggggc
    14641 tgcaaaggcc gcgcaaagta tggcggaagc gcagattagc aatatgaatg aagtcattca
    14701 acctgacgcc tccctttggg tgattaagat cgtcattctc ttggcggttc tgtggttgct
    14761 gtggatttct cgcggcgccg ggattgattt tgtaaaaggc ttctggggcc ctcagcgttt
    14821 cgtttacttt atgatgctgc ccgctttcat cgggatcttc gcagtgatcg tttatccttt
    14881 tatttataat atctgtattt cactttcaaa ttttagtctg cgcacgttcc aagattggtc
    14941 cctcatcgga gtccatcact atgttgaagc cctcagtgac ccgaaatttt atacgctctt
    15001 tttcaaaacc ttgatctgga ccgtctccaa tgtgttcttc cacgtgacca tcggcgtttt
    15061 cttagctgtg gtgattcacc aagttctccc agcaaaagct ttctggcgga ctttgtttat
    15121 tattccctgg gcggtgcctc agtacatcac ggccttgacc tggcggggga tgttcaatca
    15181 agaatacggg ccaatcaatc tcttccttca acagttttta catttgtctc cagtgcagtg
    15241 gctaagccaa cccttcacgg ctttcaccgc ttgtatcgta accaatgtgt ggctgggctt
    15301 tccatttatg atgatcgtcg ctttgggtgg attgcaatcg atcccccaat ccctttatga
    15361 agccgcttat ttggataaag ccaatgcttg gcaaagattc cgtcacatca cttggcccct
    15421 gttgatgccc gtgatggtgc ccgccgcttt gctcggctgc atctggactt ttaacaattt
    15481 aaatatcgtc tggttggtca gcaatgccgg ggaaccggga gatcaaactc atatcctcgt
    15541 gagctatgtt tataaagcgg ccttcaacct ttatcgctat ggctatgccg ctgcggtttc
    15601 catgttgatc ttcttgattt tgatcctttg gagtctcgga tccctgcgcg cgcaattcaa
    15661 aaaggagcaa aaatcatgag aagcctgccg attcaaaaaa ttcttgcgtg gttcagcatc
    15721 ctgctttttg cggtcttttc aatttatccc atgctgtatg tcttaggcat ctctctgcgg
    15781 ggggacaatg cctttcagtc ccaatcactc gagatcatca cttcgacatc cacgatgaaa
    15841 aacttccttg atctctttac tcagacggat tttttaatct ggctgcgaaa ctctttactg
    15901 gtctccgttg gcaccactct tttaggagtg gctttcgctt cgaccagtgc ctatgctctg
    15961 tcgagatttc aatttcgcgg gcgcaacatg atgctgttct ccttgctcgc cacccagatg
    16021 tttccaccca cgatgttgat tctgccgttt tatattattc tttccaagct tcacttgatt
    16081 gatagttttt ggggactttt tctgatctat tcttcaacag cgctcccttt ctgcgtctgg
    16141 caaatgaaag cctactatga cacgatccca aaagaactcg aagaggccgc cattttggat
    16201 ggctgctccc gctggctgac cttttgtaaa atcattttgc ctctatcagc tccggctctt
    16261 gtcatcaccg cgctttttag ttttatggcg agctggagtg aatacgtggt tgcggcggtg
    16321 gtcctgcaag atccacagat gtatacgctg ccgttggggc tgcgttcgtt ccaagccagc
    16381 ctcgccactc agtggggact ctatgctgcg ggtgccctca tcgtcagtct tccagttctg
    16441 attttattta ttagtatttc acgttatttg atttccggtc tgactattgg aagtgtgaaa
    16501 ggataactat gactcacatt gaattttctg aaatcaccaa aagctttaaa gacaatcaca
    16561 tcctcaacgg cgtcaatctg gaaattgcca aaggtgaatt cttagtcctg gtgggtcctt
    16621 ccggttgcgg taaatccact ctgttgcgca ctttggcggg ccttgaaaaa atggatgccg
    16681 gagatattaa aatcaatggc aaatccatta aaaatatgga accccaggac cgcgatgtgg
    16741 ccatggtttt tcaaaattat gcgctttatc cgcatatgaa tgtggccgaa aatatgggct
    16801 tcgccatgaa gctacaaaag cgttctgcca gcgaaatttc cgcccaagta aaaaaaatcg
    16861 cgcagctttt gcaaattgat catctcttag agagaaagcc taaagaactt tctgggggtc
    16921 aatgccaacg ggtggctctg ggcagagctt tagcccgcca gacttctata attctttttg
    16981 atgagcctct gtcaaatttg gatgcacacc tgcgcagtca aatgcgggtt gaaatcaaac
    17041 gtcttcatca gactttgcaa aataccatga tctatgtcac ccatgatcaa atggaggcca
    17101 ccaccatggg agaccgcatt gcggtcttga aggacggaaa aatcgagcaa attggcactc
    17161 cctctgaaat ttatcatcga cctaaaaatc tttttatcgc gagctttatc ggctctccgg
    17221 aaatgaattt catggaaggc cctctagtga aaaaacttcc ctggcctgaa gctaaaaaac
    17281 cagaacaaat tttaggcgtt cgtcctgagg tttttaaaat cagcaatgga gttccccagg
    17341 cgaatgaact cgaattagga agtttccagg tggagctttc ggaaaacctc ggaggccagc
    17401 aaatgctgca cggacgcctt gaaggacaga acctaagaat ttttgcagac tccacggagt
    17461 tttttagaaa ggatcaaatc ctccccgtaa agatagatct gacaaaatcg catttatttg
    17521 ataaacaaac aggagtaaat caacgacttc aagaaggagt ttaaatgcct tggaatggaa
    17581 ttatacaacg gctcagattt caaaaatcat tgctaaattt agcgataggc gccctgggga
    17641 ttttactggt ttctctccag gtgcacgccg gcccccgcac cgcctttgtt catctctttg
    17701 aatggccatg gaaagatgtc gccttagagt gtgaaaacta tttgggaccg gccggctttg
    17761 cggcagttca agtctcccct ccccatgaac atatccgcac gcaaaacagc ccttggtggg
    17821 aacgttatca agtggtgagt tacaagttag aatctcgcag tggcaatgaa gcggaattta
    17881 tagatatggt tcagcgctgc aaaaaagccg gggtagatat ctacgctgac gctatcttaa
    17941 atcatatgac cggaatggct ggaggcaacg gccttagcgg caccccctat tctcattatg
    18001 attattcccc cctttattct tacgcggatt ttaatcattg cagcctcaac ggcaacgatg
    18061 atatccagaa ttacagcaat cgttacgagg ttcaaaattg cgaacttctc gatcttgccg
    18121 atctggcgac aggctctgaa aaggtgcgca acaccttagc caattatctc aatcatcttt
    18181 tagatttggg agtgacgggc tttagactgg atgctgctaa gcatattccg gccgtggact
    18241 tagcggccat taaagccaag ctcaaaagac cggcttatat ttaccaagaa gtgatctata
    18301 atcccggctg ccctattcaa tacaatgagt atttttccgt aggcgatgtt atggcctatg
    18361 atttccccca tatcctggca cggggctttc aacataaaga tacgaacagc cttctccata
    18421 ttgcggatgg atttcccgcg agcgatagct ccgtaatttt tgtcactaac catgatctag
    18481 aacgtggcgg tggagtgctc agctttaact ccagccaaca acccctctat cgccttgctc
    18541 aaattttcat gatggcgtgg ccctatggct atcctcagat tttctccgga tatcaattta
    18601 atgatatgaa cgcaggcccc cccgtcggct ctgactttag aaccttgagc gtgttagatt
    18661 ccaaatctct ttgtaaggcc ccttggacct gtgaacatcg ccttcctgag gtggccgcga
    18721 tggtcaactt ccgcaatcaa acagacaaca ccttccgcgt ggaccactgg tggagcaacg
    18781 gccaagactt agtagctttc agtcgtggtc agaatggttt tgtcgctatt aatttttcca
    18841 ccaacccaat cacccgcact ttttcaacga ccatgagtcc cgggacttat tgcaatattc
    18901 tggaaggaca ttgcaatgag catttcacgg tggaaaatgg ctttgtgagt gtcatcttcc
    18961 caccgctctc tgcagtggtg cttttaaaga attaatttat gaagaaaatg acttacacca
    19021 tcggcttaga tcttggcggt acaaaacttg ccgccgccct tttaaataac aagggggaga
    19081 tgctcgattt tatcaaggtc cccgttgata tgaagcggga ggcttccgca ttgaggactc
    19141 aaaaaagagt gatcagcctg atgactgaca tcaccatgga ttttaaaaat cgtttcccca
    19201 aagagaccac gggcccgcac ttcaaaggcg tgggccttgc cagcgccggt cctttgaatg
    19261 cggcggaggg caagctcact tatcccgtca actatcccgg ctggaagatt gttcccattc
    19321 gtgagctcct gcagaaggat attcaacgcc gtggttttaa aaccaaaatc ttctttcaac
    19381 atgatgccac ggcggcagct ctcgccgagg gctgggtggg tggcgctaag aaaatgtctt
    19441 cctacgctgt ggtcagtgtc ggcacaggcg tcggctcggg cgtgatcttc cagcaccttc
    19501 ccgtgcaaag tcgcggccgt ggctctgaat atggtcatac gattatcgat tataagaagc
    19561 ttcaggaaaa ccccaccggc ctacatcact gcactgttga aggggtggct tcaggcacag
    19621 gactcctccg tcgcgcgaaa gagatgggtt tcactggtac gtccgtagag gagctagtaa
    19681 ataccaagga tgaaaaatat caaattcttt acaaggatat ggcttgggcc ttggcttgcc
    19741 tttgttacaa tctctccatt ggctataatt tagaaaagat cttcctgagc ggcggactga
    19801 ttaaaatccg cagcctctat tttgcggatt taaaagccca ctataaaagc atgatccagc
    19861 aaatgaatcc ggcctttgag tgtaaaattg aaatcgctaa aacaaaaaac accgcaggtg
    19921 ttttgggagc ggggtatctg ccttatctct cttcattgaa gaagtgagcc ccccggacga
    19981 tgggctagaa atctaaagtc aaactttaga ttattatgat aaactaaagt ttgactttag
    20041 atttaaatat accaataata tatagatatg aaaaaaaagg tctatgcgag aatactcgac
    20101 ccctttattg aattaaaaaa tggatccttt ttcttgtttg gccctcgagg ggtagggaaa
    20161 tcctatcttt taagaaccgt gattgatcaa aagaaaactt tttatatcaa tctcttagat
    20221 tcctcacttt attttcgttt acaaagaaac ccctctgaac tgatggatat gattatatcg
    20281 aagcccgtca atcaagttgt cattgatgaa attcaaagaa ttcctgaatt gttgaatctg
    20341 gtccatctta tgattgagga gcaagaactc aagtttatcc tcactggaag cagcgcccgg
    20401 aaactgaaac gaggccaagc aaatatgtta gcaggccggg ctctctccag ggaattgttc
    20461 ccactgactt ggagtgaaat cgatgatttt aatctaaaaa aatatcttca gtttggcggc
    20521 ttaccgagtt cctatctttc agcaaatccc gacgattttc tcaacgctta caccaacacc
    20581 tacctcaaag aggaaattca agcagagggc ctggtaaaga aacttcctgc tttttccagc
    20641 tttctttcta tggcggccct ctcttccgga gaacaactta actttgctaa catatccaat
    20701 gaggtcggcg tcagcgcggt caccatccgg gagtattatt caattttgga ggacacctta
    20761 ttgggatttt ttgtccagcc ttgggtgaag tcgaaaaaaa gaaaacccat ttcaacggct
    20821 aagttctatt tttttgatac cggtgttcgc aatactttgg ccgaaattgg caccctcgat
    20881 gaaaaaagca atacctgggg tcaagccttc gagcatttta tagcaatgga gcttcgggca
    20941 tttctttctt acaagctgaa aaacaagata aaactttgtt attggagatc caccagtcag
    21001 ttcgaggtgg attttatttt gggagatgcc gtggctattg aagtcaaatc ggcaaaaaaa
    21061 gttcaggcga attttttaaa aggattaaaa gctcttcaag aagaagggct ggtaaataaa
    21121 ttctatctgg tctctaatga tcctctagag aaagtgactg acggagtcca ctgcctgccc
    21181 tggcaggtgt ttttagaaaa attatggagc ggaaaaataa tttaaagtcg aaatttcaaa
    21241 aaatttttcg aatcgaggag aaccagttcg aattttctat gtgagatcca aagaaaaagt
    21301 ggatctttta atagaacttc ctaataaacg ttacgttgcc gctgaagtta aattttaaac
    21361 acgtggtcat aatccaagaa agtttccaga tatttttttc tcttgaatcg agacctcact
    21421 aagggactga aaaccaaata ataaataagg tagccacgta agtgaggccg gcttaaaata
    21481 gttgaattta ctttcaatca catacagact aatactagcg attattttgt ctctcaagtg
    21541 taatcttttt atttacggct tgttcagctt taaatttatc tagcacagta aaagaacctt
    21601 tgcaggttaa tgatccaatt cagccacctg aaatgcaccg cttttcgatt tcgagtcagc
    21661 tatcaggccc accctttaaa ccaaatttta attctagaat cgaatttgac aataaaggga
    21721 atctactact ggttccggga gaaaggatca agaggttcag aaaaagattg cagaaggact
    21781 ccaagtcctc aatgaaaaac acgatagact tgaaaagcct ttacgagaaa aaggaattgt
    21841 tgataaaagg acaaagaaaa cttctacaaa tgaaaatagt ccaactcggg aatacaccaa
    21901 gtcgggggga acagaggcat tagacaaaga ttttgaaaaa ctggatgggc catcacaaaa
    21961 gttggatgat attgaaatta aacatggcaa agacggaatt aacgctgtca agcgaccgag
    22021 aaaagatgga caagccccca ctctagaaat gcagccagaa aaaacaggaa atcgcattga
    22081 cgactcgact cggattaaaa tcaggtatag gtgatcatga aaaaggagag tagcaaaaaa
    22141 acacaaaatc ataaatttaa ttgggaagaa acttggtttc tagaaaaatt agaatacaaa
    22201 gccagtatga tccttgaact tcatattgtt ggtgcaaaaa aggagccacc tgaagatctt
    22261 attatcgaca atgtcaattt agggcaggcc taccccctcg tttgggatac ttcgaaaaaa
    22321 gttgttgttc gattttttca acctttggca tttcaacgtc tcgacgaatc gtttggcgct
    22381 gaaaacggtg gaacctatac tggtgagcga ttcaaaattt atagtgattc agaatacctt
    22441 cgctattatt caaaggtcgc ttttggcatt gttgataaac ccatacaaca ctacagtttc
    22501 acttgctcag acgacatcat tgatatttta tcaactgaag gaccagaaat agaaaatatt
    22561 cagaatgcac ccccgacata aaaatgtgag gacagaactc aaatttatcc caggtccaca
    22621 ttatttatca ctcgccagtc cgcgatccgc tttctcagta tagaattccg ccaagccaga
    22681 accgagatgg tagacagcgg tttcaatcac caggtcgttg ctttttaatt tttcagaaac
    22741 tattttagag cgtgccacta aatcgcgggc aacccctgag gcgttggctc ttgcttctga
    22801 aatataacct tgggaaggct ccttgccgct aaatttactg atgcgcgggt gaatatcctt
    22861 caccaaggca ttcagagcag gcgaaccggc atcttctcct ttgagagttg aaaaggccgc
    22921 tttcacggcg ccacaagatt catgagccat caccactagc aagtgagaac cgatatgttc
    22981 taaggcatac tcaacactcg cgatggcggc attatccagt gcctcaccgg cggttcgcac
    23041 cacaaaaatc tctcctagtt tttgatcaaa taggacttcg gggggaactc gagaatctga
    23101 gcaagacaaa acaatcgcgt ggggtttctg accgcccaca agacgttggc gatctcgatt
    23161 ggactgccca tcttttctta agaaaccttt tacgtaacgg ctatttccat ttttgagcca
    23221 gctaaaagag gtcttcgcat tcacaccaga atcggcggct gcatgggagg cttctttatg
    23281 cgtcgacgtt tcagattgag aataagaaac gggagtgaac gaaaggataa gagacagaaa
    23341 taacgatttc ctgatgccat tcataaatac acctcttctt tggtctaggt tcctcctctc
    23401 ttcgtcatta tgaagccaga ctttaggcca aggggaattt ttcagcgaca cccggattgc
    23461 cggaaaataa tggtattgta actgtctcaa gaaaagaccc agcttttcgc tcggccgaag
    23521 taaagattct atttccgtct ataattttaa catggctcaa gaattcagtg gcgttgagtt
    23581 cctaggcttg ttaaaagccc accttgttta gatattcaaa taattcaatg gagcatttat
    23641 gaagaacgga tttatagccc tggtcctaat aatgagcgcc tcttgggctt gcgctgaaaa
    23701 cctccaccct tttcacagcg acggctgcag cggttctcca gattccattt taggaacaag
    23761 ttatatccat tgttgtattg cccatgactt tgaatattgg gttggtggaa ctaaaactca
    23821 aaaaaatcaa gctgacgaaa atctcaaaca atgtatccag agaacaatac cttccttgaa
    23881 gatggttggc tcaatttata aacttggagt tatcatcggt ggtactccca ctttctttgg
    23941 agccatcaat agtcctttta cctggcgttg gggttatggt tgggaagagg atcatggata
    24001 ttctccatcg actaaacagg aattagaact cgcactgatt gaccttcaaa ggatggaagc
    24061 agactttgac gcccacgaaa gagccctcaa tgaaactcag ggtttcgctt ggagatttgg
    24121 gaaccttaag atttcccctg aacaatttaa gcaaataaag aaagaccttc agaaaaaaat
    24181 taacagcatc ataagtatat tgaatgacac tgatttatga tttttcaatt taaaaggaaa
    24241 cattgcttat ttcaatttat ggccttaccc actctcagta tcaaaatatc gccattccta
    24301 tagccaagag tttttctgcg aagttttcca gtgattttga aacacgagtt atttatcaag
    24361 ccacccagga agatctgtac tcggcactga ataaccccca aagcgtgggg gtcttctggc
    24421 tgagtcattc aaatagcgga attcaagctt cagggatagg aatgggcgga agtatcgttg
    24481 attctaaagg tgtggatgtt tctagagtct ttacaaaaat ccatccaaac ataaaatgga
    24541 tcggagtcat tggctgcaaa gctttaccga tctttaaggg gttcgcagac aaaggttttt
    24601 ataaagacaa aaatattttt ttacatgatc aggcctttaa ctggaacgcc cttgtaggta
    24661 tggaccacag cacctccctt actagctttt ccggcctggt catctcctcg agcacgaaac
    24721 tagctctaac aaatatcgac tccgatagaa atttcaaaaa tgccactact tatctccagc
    24781 gtttaaagcg tgaaaaccaa ctcactgatg ttcaaactcc agcagactca aacgttctca
    24841 gatttaaagt gaaacgggtt ccagatcatt ttaataagaa tgaaatggtc gcagtacagg
    24901 tcttggtgaa cagtttaatt ctacttactg accctgaatc ataagccctt ggggattaca
    24961 caaaatgtgt atcgagtgct gcattagata aacgcaacgc atccgaaaaa ggtacggcgt
    25021 accctcaata aagtaaaaac ttcttccggg ttttcagcca ggccttctcg tccttcttca
    25081 aagctttttc taaatccgct ggggtggcct tggggttctc gatccactcc cgcaattctg
    25141 gtccgccgtt gatcacatcg aaggctaatt tccctacagt gtattcataa gcgaaatcac
    25201 gatagagagc gtaattggga tgaatctcgc gaatgcactt cagcatcaag gcgatcagac
    25261 gatagggttt aaataactga ggctgataag cagcgatgtc cgcgtggatt tggaaaccat
    25321 ggcagagttt cttctcgtgc ttatgaaacg tcggttcaaa gaagcattcc cgcagaataa
    25381 ttcccttcaa ccattgcggt gcttttttct gcattcgtcc taacacttcc gtgaaatcaa
    25441 tatcactcgc tccgacctgc tccaaagccc gggtcgttcc gcgaccttca ctcaaggtgg
    25501 tgccttcgat catcacggtg ccgcaataag cccgagccat attcaaggtc tgggcattcg
    25561 gagagggatt cacccaagcg cgatccatgg gccagccaaa tcccggcttc ttttcgcagt
    25621 gatagccttt cattttaatg actttcaaat cgagatccaa tttgaaatgc tctttgtaaa
    25681 ataaagccat ctctccacaa gtcagaccgt ggcgcatggg gcactcagcc gctccaacaa
    25741 aggactccca accggggcgc aaagaaaaac cttcgacttc gcgacccgca ggattaggac
    25801 gatctaaaat aatcacttgt tttttacgtc gagcacattc ctgcattacg taaagcaaag
    25861 tggtgatgaa agtatagata cggcagccca gatcttgcag atcaaaaatc gccacgtcaa
    25921 aagtctccat catctttggc gagggagttc tgactttgcc atagagggaa tatatcggaa
    25981 ttttatagtc cggatgaatg aagtcctcac tctcgatcat attgtcttgt ttttcaccct
    26041 tgactccgtg ttggggacca aaggctgctg acaagccgat ggtcttatga agaagatcaa
    26101 aactgtgacg aagcttttca tccacactag caggatggca gatcagagcg acccttttct
    26161 ttttcaaaac ctggattaat tttttgtcct ctaaaagaac ttccacaccc attttcattc
    26221 ttcagtccct cgctgtaaaa taaaactctt catcaaatga aaatcaggct gagtgccctt
    26281 gtgagcaaga gcgcagtagt gtttcgtgcc atttttctcc agaagaatcg ccgtcaaccc
    26341 cacacgaaat tgactataga aagttttatt ttcaagctcc accaaaaggc gttttttcac
    26401 ggcatcccag cccatacttt tcaaaacaaa ctcctcagtg ggagccgccg gctgaggcag
    26461 gcgatagctt tcaaatttat aagcattcca agcagggttt aaagagaaat taaattcaaa
    26521 atattttgaa ctgcccaggg gacttaaaaa agcctcgaag cacgtggcct gccaaaggcc
    26581 gtcttgccgt ggaacctgag cttctggcca agaagcggaa gttttaggca attcgaaaag
    26641 ctcagcgctt tgctctaaaa aaccaaactc aagataaaga gaagaatctt taaattgaag
    26701 atccgcctcc agcagaaggg tttccccact gcaaagaggt tccatagcat tgggatcact
    26761 gaaaggaggc gaaaatgaga ttagatttgg cataagcaaa agtctagcaa gggaggtttg
    26821 ccttgggaac caggaagtcc gtaatgcaaa gcttcccgcg tgccaaagag gctcaattag
    26881 cgtataccat aaaggtattc tctttacttc aaaggaaatg ttcatgtctg aatctactca
    26941 atttgatctg gtaattatcg gctctggacc tggtggctat gtgggagcga tccgcgcttc
    27001 gcaattagga cttaaaaccg ccgtcgttga aaaagaaagc acctttggcg gcacttgcgt
    27061 ccgcgtgggc tgcatccctt ctaaagccct gcttgaaagt acggagcact tcgaagccgc
    27121 tcaacatgat ttcgccagcc atggcatcaa ggtttcaaaa gtggagctgg atttaacgac
    27181 tatgatgtct cgtaaagaca aagtcgtaaa atccaacacg gatggcatct ctttcttatt
    27241 taaaaaaaat aaaatcactt ctttccaagg aactggaaaa atcctgaaag ccggctcagt
    27301 ggaagtcact gcggctgatg gctctaagca agtgatcttg actaaaaata tcgtgattgc
    27361 tacgggatca gttccagtcg aactcccctt tttaaaatat gatgaaaaaa agattgtctc
    27421 caacacgggc gctttgacct tgttagaggt tccgaaaacc atgatcgtcg tcggcggcgg
    27481 cgtgatcggc cttgagctgg gctccgtctg ggggcgcctg ggttcccaag tgacagtgat
    27541 tgaatatgcg gatcgtttgg gcggtcctat ggatcaggat tgcatgaatg tccttaaaaa
    27601 gaatatggag aaagccggag ttaagttcct cctttccaca aaagtgacag gttcgaaagt
    27661 cactggtaaa ggggtggaaa tcacttacga agcactcgct gatggtaaag ctcaaagcat
    27721 gactgcggat gtggttttag tctctaccgg ccgtaaagcc ttcacggctg gagtgggatg
    27781 tgaagaggtg gggattcaaa aagacccaca aggtcgtatc attatcgata agcactacca
    27841 aacaaatgtt ccgggtatct atgccattgg tgatgtggtg gctgggccca tgctggctca
    27901 caaagccgaa gaagaaggcg tggccttagc agagcttctt tcgggccatg ccggccatgt
    27961 caattacgag acagttccag gagtgattta cactcatccg gaaatttctt ctgttggaat
    28021 gactgaagag caggccaaag ctcagggcct tgaaatcaat gtcggcaagt ttccgtttat
    28081 ggccaacggc cgcgcccggg cgaaaggttt tacagaggga tttgtaaaga ttatcgctga
    28141 taaaaccacc gatcgcattc tcggttgcca catggtaggg cccggcgttt cggagctgat
    28201 ccatgaagtg atcgtgtgca tggaattcgg gggcagcagc gaagatctgg cacgatcttt
    28261 ccatgctcac ccgaccctta gcgaagtagt tcgggaggcg gccttagcgg tggaaaaacg
    28321 ccaacgtcag atgtaaaatg taagattctc gaaatcaaca acttacggtc aatcggtttg
    28381 gttaaataat ccgaacatcc cgcctgcaga cagcgctctt gatcttcttt gagcgcatgg
    28441 gcggttaaag caatgatcgg gcgtttatag tcctgctcgc gaagtttagc tgtcgcttcg
    28501 tacccatcca tttccggcat ttggatgtcc atcaagacga cagcatagtc tccggcaaga
    28561 gctttttgca ctcccaaaac cccattctcc gccacttcca cctgggcccc cgcacccgtc
    28621 aaaaagcgac tgatcagcag ttgattaatg ggggcatctt ccactaaaag aactttcttc
    28681 cctgaaaggg catgggcttc atcagccgct ggagattcta gatctttcac gataaaatcc
    28741 tccactttgt cagtaaattt tgaatctagt aaagtatctg tttcaatctc aatcacaaaa
    28801 acacttccct gacctctctc acttttttct aagaggacat ccccgcccaa cgcctgagcg
    28861 atctttcgag aaagtgccaa tcccaggcct gtgccaccaa aacgccgcgt gtttgaacta
    28921 tccacttgca caaagggttg aaagaggttt tgagtatgag actcttcaat tccgacaccc
    28981 gtgtcttgta cgcggaaagc cagcactgaa ggacgtaaaa gtgtcgcctc aatccattcg
    29041 gcctcaacca cgactttgcc tttttcagta aacttaatgg cgttgccaat cacattcatc
    29101 aaaatctgcc gcacccgggt cgggtccgaa aaaatgtgct caggaaccag ggatttaaaa
    29161 cgaaattcca aaccgacacc tttcgatttc gctttcacca tcagtaaatc tctcaagtca
    29221 cggagtaagt ctccaagatt cacatcaata ttttcaattt ccatatgccc tgattccact
    29281 ttggaaatat ccaaaatctg atcaatgatt ttcaaaagct gatgaccact gcgatggata
    29341 gtgtggacat aatgagtttg ttcttcaaaa cttaagtgag gacttaacat gagttctgca
    29401 aatcccaaaa tagcactcag gggagtgcga atttcatggc tcatgtttgc cagaaaatga
    29461 gtttttacaa tgtttgcgga cacggccatc ctctcggcct cgaccagttt tctttcggcc
    29521 agcttccgat cgtgaatatc ggcacaagtg ccaatccagc ccacccgcat atcaagagta
    29581 ttcaactccg ggaccaccag cagccaatgc caacgcatct gattatcgtg tcgccgtact
    29641 cgacactcga tttgaaaaga ctcaccgctt ttcatcgatt cattccaaga tcttagaaaa
    29701 ccgctcaagt cgcttatatg aattgctttt tgccagccca gaccaatact ttgttcctta
    29761 gaaagtcccg tgtagcttgt ccaggatttg ctaacatact ctagcgtccc gtcgattttc
    29821 gatttccaca tcatacagaa gccccccggt ctctccagaa aacagaaatt aacttttgtg
    29881 aatatacagt gtatttttat gaaatagaaa atgacgattt cgtcacatgc acattattta
    29941 accatttttc ttggcctact ttttgctaga aatagagaaa ataatctagg agatgattca
    30001 tccgtagtca gttcgacccc aaccgctgat ccaaacttct aaggagcgtt ctgtgagctt
    30061 agtaaaattt tcattcttat ttttgatgac cttcgccttt tggactcctc aggccgatgc
    30121 cgaagtgtgg accacagtga atacctggga cgcacagtgg gaaaaaaatt atacagaatg
    30181 ggttgaaaag acttggtcgg ctgacttctt ctctcgcgct tcgctgccga atggtcaaaa
    30241 caatccctat tatggagtcc gtgcggattg cgccgataca gtctattcga tgcgcttgat
    30301 ctactctttt gaaaataaac tgccctttgc ctttcaggat ccgacctcaa ccggcgctct
    30361 tattagtaac tcgatgaacc gctggaatag atattcggac gaattaaagc gttttagaag
    30421 ttttttagct tatgtcatgg gtatcgtaag caccacctca cttcctaatg acactttccc
    30481 ggtgccggtg agccgcgatt ggattcactc tggaggattg attcgcacca ctaaagtgaa
    30541 tcaccactct tggaccattc gagaaatcca agctattgga gtgcctcatt tgatctttaa
    30601 ctcgaccgtt ggtcgtcaat ctggattcag tcttcaagaa cgcgtttctt ggccgaatcc
    30661 aaaatgggtg tttgaaggcg acttctcagc cgcaagcaat gcagggtttc gttattggag
    30721 acctctcgaa tttatcaatc gccccgtctg ggaaactccc ggatacagcg aagagcaatt
    30781 ttccattccc cttggtaaat ggcagaaaac tcttcaaaac aaattggctt tgcatcaaga
    30841 aaccagtatt caaatgctcc gccgtttgtc tctgaccgct tgcaatgcgc tcaaggaccg
    30901 catcaccgct gtgaatgacg gtttgacatt tctgcgctcc ctgccttcta atcaatgtat
    30961 gagttttgaa aattacgata cgtattccac accaaatcgg gatcaacgac tttttgatga
    31021 catggcggca cttcgacggt cttacaaaga catccttcac ggcaaaaatt ctttaccgga
    31081 ggatatgaaa attcaactga ataaggtatt cccattgatt cagagatccg cgcaaaaaga
    31141 aaccagtgtc atgcaggtgt caaatatgga tacagcttcg tggtgcccgg tggaattttc
    31201 cccaggcgaa aaaattgatt ttgctgaagc aaaaagaagg ctctttttgg gccaaatgtc
    31261 taacaatcct ctcgaggaag tggaataccg ctggggcact gtgcagggac cttcagcgag
    31321 ggcgcaagct tgtgaaagct gggatatttg gaaacctgat ttaaaactag cggattaaat
    31381 tattatggag cgtaagcgtc ggactcgctg gagccttatt atagtcagtt tattttttat
    31441 tagccttgct gtggattatt ttttcttgca ccttcttttt cgacaaagac tgaaagaaca
    31501 acagcagcaa aaagaacagc aagaagaaat gactcctctt agggagcaaa gccaaagcct
    31561 gatcctgaag ccgaacgtaa gctctgcagg gactgaagat aataggaagc tgcgctcgcc
    31621 acttcaagag gagtttcaag acaaactcaa aaagtgtttg ggcccaaagc ttggaaag
//
LOCUS       DATBTE010000236         3585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1328205, whole genome shotgun sequence.
ACCESSION   DATBTE010000236 DATBTE010000000
VERSION     DATBTE010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328205"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VIG33_08720"
     CDS             complement(<1..598)
                     /locus_tag="VIG33_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8270959.1"
                     /translation="MNNYYVTAFYKFLILENPEQAQKDLETKAQELNIKGLVILGREG
                     FNSTCAAVSLESLEIWKVFICEYFKVTDVFFKDSASPKAPFRRFKIKIREEIVTTGIP
                     EIAPPEGKNHHLSPLEWNKVLKEENDFVMIDTRNWYEFKIGTFKGALNPNIEKFTDFP
                     EYIEAQGIQKDKKMLIFCTGGIRCEKGILELQKQGYENV"
     gene            complement(695..1174)
                     /locus_tag="VIG33_08725"
     CDS             complement(695..1174)
                     /locus_tag="VIG33_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270960.1"
                     /translation="MSIPKSFFLLLAPKNEKPGPIPGFAKDIRGEALENEADEADILG
                     DQDLTEKMDQSDDENLGAKSKSLDILEEEPEDFLAGEVDVKEQMDRAANAMEASIQGL
                     QNRAYEDSPQTELGAAFRNTPGGIEEVEMKTDQDLALHHHSPHARKRPTRKKSTTPS"
     gene            1316..2923
                     /locus_tag="VIG33_08730"
     CDS             1316..2923
                     /locus_tag="VIG33_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEY8270961.1"
                     /translation="MLAPPLIQKEIQRRRTFAIISHPDAGKTTLTEKLLYHGGVIHET
                     GEVKGKRGTKAVTSDWMELERQKGISITSSVMTFEYNQLRVNLLDTPGHKDFSEDTYR
                     VLMAVDSACMLIDVAKGVEERTKKLYEVCRLRQVPIFTFVNKLDREGKDPLTLIDEVE
                     KTLNMQCYPVTWPLGIGQRFRGIYNRISQELWIYDQRREEVEDYKVIPFVPGKDNEIL
                     FDYLDRESAEQVMDELELIEGALPPFDVQAFLAGELSPVTFGSAKQNFGVDTFLNFFS
                     QYAPGPQSRKTKADITIDPCDANFTGFVFKIQANMDKRHRDRIAFIRICSGKFDRGMK
                     VQHSRLERELRLSYASQFIAADKATIEDAYAGDIVGVGDTGNFAIGDSVSATSKVQFE
                     DIPKFAPELFGKLSVRDALKRQKMQEALKHLSEEGAIQLFYDPVYGPQDPIVGAVGEL
                     QFEVLIHRLNDEYGLEVKLTRLPFSVARWPRTEDGKPVEQLNGGFTIYRDMIDQPLIL
                     LNQEWDLNWAQRENPKIHFVSSISRAR"
     gene            3041..>3585
                     /locus_tag="VIG33_08735"
     CDS             3041..>3585
                     /locus_tag="VIG33_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEY8270962.1"
                     /translation="MFENLSDKIMASLKKVRGQNKISEANIEEAIKEIRLSLLEADVN
                     FKVVKSFIDKVKTKALGQEVLSNINPGQQFVKIVHDELVNVLGGGAVDINVRENPSVI
                     FLVGLQGAGKTTTAAKLSLYVRQKLGKKPGLVPADIYRPAAVDQLQILASQNNIPCFS
                     TQAGQKPEEILEQAKKWARDN"
BASE COUNT          970 a          726 c          821 g         1068 t
ORIGIN      
        1 aaacgttttc atatccctgc ttttgcagtt caaggatgcc tttttcgcaa cgaatgccgc
       61 cggtgcagaa gattaacatt tttttatctt tttggattcc ctgcgcttca atatactcag
      121 gaaagtcggt aaatttttcg atatttggat tgagtgcgcc cttaaaagtg ccgatcttga
      181 attcatacca gttgcgcgta tcaatcatta caaaatcatt ttcttctttt aaaaccttat
      241 tccactccag cggacttaag tgatgattct ttccttcagg gggcgcgatt tctggaatcc
      301 ctgtggtcac aatttcttct cggattttga ttttaaaacg acgaaaagga gctttcggag
      361 acgctgagtc cttaaaaaac acatccgtta ctttgaagta ctcacatata aaaactttcc
      421 aaatctccag gctttctaag gaaaccgccg cacaagttga attaaaacct tcacgaccaa
      481 gaatgaccaa gcctttaata ttgagttctt gggctttggt ttcgagatct ttttgagctt
      541 gttcaggatt ttcaagaata aggaatttat aaaaagccgt aacataatag ttattcattt
      601 aagcgctcca attatccggt ctgggctaca aacccagggg agttgaaatt gattttgcgt
      661 tttataaggg ataataaaaa gcttcgtcaa gggcttatga aggagttgta gactttttgc
      721 gtgtaggcct tttacgggca tggggactgt gatgatgaag tgctagatcc tggtcagtct
      781 tcatctccac ctcttcgatt ccgcccggtg tattgcggaa agctgcgcct aattccgtct
      841 ggggactgtc ttcataggct cggttttgta atccctgaat agaggcttcc atcgcgtttg
      901 ccgctcgatc catttgttcc ttcacgtcaa cttcgcctgc aaggaagtcc tcaggttctt
      961 cttctaaaat gtcgagagac ttcgacttgg cgccgagatt ttcgtcatca ctttgatcca
     1021 ttttttcggt gaggtcttgg tcccctaaga tatctgcctc atctgcttcg ttttctaagg
     1081 cttccccgcg gatgtccttg gcaaatccgg gaatgggtcc gggtttctca ttctttggtg
     1141 ctaaaagtag gaaaaaagat ttagggatgc tcataaatcc tcctctggct caagagaatg
     1201 ttccattaag caccagtttc gcagagagcg gaacatgtat ttagtcataa aactcttgtc
     1261 ccaatgattt ccatcattta taacagtttt ttgatctcaa agttgaagga taaacatgct
     1321 agcgccccca cttattcaaa aggaaattca aagacgtcgc actttcgcga tcatttccca
     1381 tcccgatgcg ggaaagacca ctttgaccga aaaactactt tatcatggag gggtgatcca
     1441 tgaaacggga gaagttaaag gtaagcgggg cacgaaagca gtgacttccg actggatgga
     1501 gcttgagcgt caaaaaggaa tttccattac ttcttcagtg atgacgtttg aatacaatca
     1561 actgcgggtg aatcttctgg atactcctgg ccataaagat ttctctgaag atacttatcg
     1621 cgttttaatg gccgtggatt ccgcttgtat gcttatcgac gtagctaagg gtgtcgaaga
     1681 gcgtactaaa aagctttatg aagtttgccg tcttcgccaa gttcctattt tcacttttgt
     1741 aaacaaactg gatcgtgaag gtaaggaccc tctcactttg attgatgaag tcgaaaaaac
     1801 tttgaacatg caatgctatc cggtgacttg gcccttaggg atcggtcagc gttttcgtgg
     1861 gatttacaat cgtatttcgc aagagctgtg gatttatgat cagagacggg aggaagtgga
     1921 agactacaaa gtgattcctt tcgttccagg taaagacaac gagatcttat ttgattacct
     1981 tgaccgtgaa tccgcagagc aagtgatgga cgaattagaa ttaattgaag gggctttgcc
     2041 tccttttgat gttcaagcat tcttagccgg tgagctgtcg ccagtgactt tcggttcggc
     2101 aaagcaaaat ttcggagtgg atactttttt aaacttcttc agtcagtatg ctcccggccc
     2161 tcagtcccga aagactaaag cagatattac tatagatccc tgcgatgcga acttcacggg
     2221 gtttgtgttt aagatccaag caaatatgga taaacgtcat cgtgatcgca ttgcttttat
     2281 tcgtatttgt tctggcaaat ttgatcgggg catgaaagtt caacattcac gccttgagag
     2341 ggaacttcgt ttgtcctatg caagtcaatt tatcgcagcg gataaggcca ctattgaaga
     2401 cgcctatgcg ggggacattg tgggtgtagg ggacacggga aatttcgcaa ttggagattc
     2461 ggtgtcagct acaagtaaag ttcaattcga agatattcct aagtttgcgc cggagctttt
     2521 cggtaaactt tcggttcggg atgcgctgaa acgacagaaa atgcaggaag ctctcaaaca
     2581 tttatcagaa gaaggtgcta ttcagctttt ctatgatccg gtttatggtc ctcaagatcc
     2641 tatcgtgggt gccgtagggg aacttcaatt tgaagttctg atccatcgct tgaacgatga
     2701 atacggtcta gaggtgaagc tcacgagact ccctttctct gtggctcgtt ggccgcgcac
     2761 tgaggacggc aagcctgtgg aacagctcaa tggaggtttt accatttatc gtgatatgat
     2821 tgatcaacca cttattttac tgaatcagga atgggatctg aactgggctc aacgtgaaaa
     2881 tccaaaaatt cattttgtat cctctatcag tcgtgcgcgg taggtatgaa taatattttt
     2941 agcgttggta ataaccctgc gcattacatg tcttggtttc ggaaacttct cttggatgat
     3001 agtaaaaaaa agcttaacgc ttagatttta gaggaaaaag atgttcgaaa atttgtctga
     3061 taaaattatg gcgagcctta aaaaggttcg tggtcaaaat aagatctcgg aagccaatat
     3121 cgaagaggcg attaaagaga ttcgcttaag tctgcttgaa gcggatgtaa actttaaggt
     3181 ggtaaaatct ttcattgata aagtgaagac aaaggctctc ggccaagagg ttctttccaa
     3241 tattaatcct ggtcaacaat ttgtgaaaat cgttcatgat gaattagtga atgtcttggg
     3301 gggcggagct gttgatatca acgtgcgcga aaatccaagc gtgatttttc tcgtcggcct
     3361 gcaaggcgct ggtaagacga ccacggctgc aaaactcagt ctttatgttc gtcaaaagct
     3421 tggaaaaaag ccaggcttgg ttcccgctga catctatcgt ccagcagccg tggatcaatt
     3481 acagattttg gcgagtcaaa ataatattcc ttgtttctca actcaagccg gtcaaaaacc
     3541 agaagaaatt ttggaacaag cgaaaaaatg ggcgcgcgac aatat
//
LOCUS       DATBTE010000237         8815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_645192, whole genome shotgun sequence.
ACCESSION   DATBTE010000237 DATBTE010000000
VERSION     DATBTE010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645192"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VIG33_08740"
     CDS             complement(<1..501)
                     /locus_tag="VIG33_08740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8270963.1"
                     /translation="MDVIIVGGGFYGCCLAIEMASKGKSVLLLEREASLLSRASYSNQ
                     ARIHGGYHYPRSFLTGVRSRENFKLFVEAFEGAVVRNFTKLYAIAQNSKVTAKQFFEF
                     CNRIKAPIKMASGQHIQLFSKESIESVFEVEEYAFDSQRLAQIVQKQMAELGVEVRRN
                     STVTEMV"
     gene            complement(531..1736)
                     /locus_tag="VIG33_08745"
     CDS             complement(531..1736)
                     /locus_tag="VIG33_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8270964.1"
                     /translation="MSSKVAVRVQNLSKYYKLYKKPLDRVKEALHPLRKQYHRGFEAL
                     KNISFEINKGQTLGVIGKNGSGKSTLLKILTKVLTPSSGSVEVNGKVAALLELGAGFN
                     PELTGIENIYFSGTLMGYTEKQIKERIPHILEFADIGEFSSQPVKLYSSGMFARLAFA
                     VAINVSPDILIVDEALSVGDAAFSLKCLNKMNEIARAGSTIIFVSHDIQSVKTFCDQV
                     LWMHEGRIQDFGESNKTCSAYLEYMYSNTLRTHEDEEGGLGCRTKLSRWGTGEVMVTD
                     FHLHSGKVKDYIFNYNDEVEVKIEALSHVANREGRIGFSIKNEKALDIINWMSETLLF
                     DKERTYKFDIFFKNILAPGKYFLILVVDDLPNGKYYDYVENAVLFEVVADKTIHSLVL
                     PDMRVVFQS"
     gene            complement(1739..2530)
                     /locus_tag="VIG33_08750"
     CDS             complement(1739..2530)
                     /locus_tag="VIG33_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8270965.1"
                     /translation="MREFLKTIILQRRIIWSLAKSDFRSRYLGSVLGILWAFLLPLLN
                     LGIMWFAFQQGFKSSPIKGLPFILWLVTGMFPWTFFAEAVQNASNCIVEKSFLVKKVV
                     FDVELLPLIKIFASAILFGFLSLVMLLLFLVYGYGPDKYWLQLPYYAFCLCLLILSVS
                     WLTSAVVVFYRDLGQVITMLLQLGFWVTPIFWSPENLPEDFKFIIFLNPVNYVVSGYR
                     DSLISKVWFWELPLQTLFFWLFFLVFSFFGLFVFKRLRPHFADVL"
     gene            2671..3834
                     /gene="wecB"
                     /locus_tag="VIG33_08755"
     CDS             2671..3834
                     /gene="wecB"
                     /locus_tag="VIG33_08755"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEY8270966.1"
                     /translation="MKTILVVFGTRPETIKLAPLILRAKKDSHFNVLVCSTGQHREMI
                     KPLFNFFGIKPDFDLDLMKPGQSLTDISVGIMRELSAIFETHTIDGLVVQGDTASCFI
                     ASLVAFYHRIEVFHIEAGLRSGDLFSPYPEEFNRKGTGLIAKLHFAPTEAAKANLVHE
                     HYPASDIFVTGNTGIDALFEAQRKIQESPELKLQFDKTYSFLNPQKKLILVTVHRRES
                     FGAPIEEIMKGLVALAQREDVEILLPLHMNPQVRKSAEQIFNGHSCWIDQTTPPKKDQ
                     TRIWLCEPIDYVPFIYLMDRAHLILTDSGGIQEEAPSLGKPVLVAREKTERPEAIAAG
                     TSKLIPLEARAFLSTVEELLDTPALYDQMAKAQNPFGDGRACERILNILAERF"
     gene            3834..5666
                     /locus_tag="VIG33_08760"
     CDS             3834..5666
                     /locus_tag="VIG33_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270967.1"
                     /translation="MKLSHNFLLFLQYAVFAMILFGIKLALIHFYGNATPYWDQWDAE
                     AANLYKPFLEGTLTWKSLFTPHNEHHILITKLLALAELKLNGLWNPLLQMVVNAALHI
                     VAILFIVDQLTRVIGRKFLTPLLIFSLVLFSIPYAWENTLAGIQSVFYFVLLFSTACL
                     WLTTIYSPLSLRWWCGISFAVLAFFSLASGIFALAAAATIGLFLYFTGLQKTPQQLVA
                     IVILSALFALGAALTPTLAYHAPLKAASPLQFLDALKTILAWPASSGFLAAFIRNLPA
                     LLFVRSMMKTRPSAKDRRWFLLALIVWMLGTAVSIAYGRAGGSTASRYLDLFAIFILI
                     NFACLIFLSYERIKKRHFWTMMGTALWTLAISIALFLPAMEKIPSELMDKRDTGSFQE
                     INTRNHLLTGDAKDLRDKSFLHVPYPNSERLISILASPSVREILPHNIRTPFTPTSSD
                     IKPADSLVTNGYDPKTPGRTHVLLGSYNNQGNDNTGKASITFAGNPTATVMAIPIAGF
                     PLEKGMELALEQNGKQSPLAITKDPQESWSIAYAHIQEGPFTLHLMDSSPSAWVAIDA
                     PSVAGRMDELVFILTENFYVFISSGIALAALIVLAQRFRFFGKS"
     gene            complement(5636..6382)
                     /locus_tag="VIG33_08765"
     CDS             complement(5636..6382)
                     /locus_tag="VIG33_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8270968.1"
                     /translation="MTDNTVAKKIDITLFFPLYKDEGTVESITAKSVKMLSQITENWE
                     ILIIDDGSPDRAGVIADALSAANPKIRVIHHETNLGYGAAIRRGLAEARGEWIYFTDG
                     DDEYDVFEFLRIHKIRDFYDLIITFRYKKLYSSKRQFISNVYNIVLRFLFKIRYRDIS
                     TGLRAMRKSLVQELILSTNSPFIGAELTIKSDLKGYRIGEMGIQTFPRQFGQGASTSL
                     KNIVATIKDMFKIYNEIFSDTYDLPKNRNR"
     gene            complement(6396..7151)
                     /locus_tag="VIG33_08770"
     CDS             complement(6396..7151)
                     /locus_tag="VIG33_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8270969.1"
                     /translation="MKPTSTALIVGGSSGLGRALAELLAKKGFGLALVSRDKEDLQAL
                     QSSLQTVHGVRVFIYPTDLLDPALSAERVVEKVSEDLGSIDYAFLVAGGSFTGDEKVP
                     SLSAFSHSLRLNFEAIGLFMNAILLLREKAVCRSILVISSIAAVAPRSQNSSYAAAKK
                     ALESYAISLRHYCFQAQIPTNIQVVRLGYMESGFTRGKTLLFPIAKPQAVAQFIFKLK
                     DRDFGLRVFPSFWNLILFILTKLPWAVYKRLRF"
     gene            complement(7148..8461)
                     /locus_tag="VIG33_08775"
     CDS             complement(7148..8461)
                     /locus_tag="VIG33_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY8270970.1"
                     /translation="MTENQIIHSFDATFSVEGELFITEKYRDLYFAMSERPFISRGAG
                     VSYPLASAGVGVRSIVSLQFNRILEFNNETGVVKAESGLSVGELLKFLSQRGWWLPAI
                     PGHPSITLGGCVAANVHGKSQYHSGLFADSVLEMELFHPRHGKILCLPKSLPFQLTAG
                     GFGLTGHILSVSIQCKPLTGKSLVRTSLPCANIFECIELMQNEKDQYQQVYSWNDLNQ
                     KGEKFGAGYVYYEKFGAEAVPDIQEFNVLYSPPRSSLMDQVFAWTLKNRISQAYSMKE
                     SFAPKSQSLGILAGAFPINGKEIYHRLFGKNGLLESQIIIPHAELKPVIEKLSLAIAE
                     FGGAVSLGSLKIFRGQQNYINFLTDGICLALNVPNSARALDLFRKIDEICEDHHCLSN
                     IAKDSRLTQDVVAATYEGYQQFKKDIFDFDPSKTVSSALRVRLGL"
     gene            8505..>8815
                     /locus_tag="VIG33_08780"
     CDS             8505..>8815
                     /locus_tag="VIG33_08780"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8270971.1"
                     /translation="MNEKRAAYTQTRYSLDAKRTLVWKVFAPFLQKKFQIQGHVLDVG
                     CGFGDFINHIEAPRKSALDANADMQNFLQPGIDFKDGLTSDLKTFFKANDFDWIFSSN
                     LL"
BASE COUNT         2569 a         2117 c         1738 g         2391 t
ORIGIN      
        1 caccatctcg gtcacagtag agttccgtcg aacctcgact cccaattccg ccatttgctt
       61 ttgcacaatc tgcgctagcc tttgagagtc aaaggcatat tcttcaactt caaaaacact
      121 ttcaatactt tcctttgaaa acagctggat gtgctgacca ctggccattt taatcggagc
      181 ctttatgcga ttgcagaact cgaaaaattg cttagcagtg actttagaat tctgggctat
      241 ggcatagagc ttggtaaaat tacggaccac agctccttcg aacgcctcaa cgaataattt
      301 gaaattttcc ctggatctga cgccggtcaa aaaacttcga ggatagtggt accctccatg
      361 aatccgcgct tgattagaat aactggcacg tgacaagagg ctggcctcgc gttctaaaag
      421 gaggaccgac tttcctttgg aagccatttc aatagccaag caacagccat aaaaaccacc
      481 tccgacaata atcacatcca tatgtgccct acttacaact tatctaaaaa tcagctttgg
      541 aaaaccactc tcatatcagg caaaactaaa gaatgaatcg ttttatcagc tacaacctca
      601 aataaaaccg cattttcaac ataatcgtaa tattttccat tgggaagatc atcaacgacc
      661 agaatcagaa aatattttcc gggtgctaaa atattcttaa agaaaatatc aaatttatag
      721 gtccgttctt tatcaaataa aagggtctcc gacatccagt tgatgatatc caaagctttc
      781 tcatttttta tagagaatcc aatgcgaccc tccctattcg caacatgact taaggcttcg
      841 atcttcactt caacttcatc attataatta aagatataat ctttaacttt acctgaatgg
      901 aggtgaaaat cagtcaccat gacttcgcca gtgccccaac ggctcagttt ggtgcggcaa
      961 cctaaaccac cctcttcatc ctcgtgagta cgcaaggtat tcgagtacat atactctaaa
     1021 taggcactgc aagttttatt ggattcgcca aaatcctgaa ttcgaccttc gtgcatccaa
     1081 aggacttgat cacaaaatgt cttaacactt tgaatatcat gacttacaaa aatgatcgta
     1141 gagccagctc tcgcaatttc attcatctta tttaaacatt ttaaagaaaa agcggcatcc
     1201 cccacactga gagcttcatc cactatcaaa atatccgggg acacattaat agccacggca
     1261 aacgccagcc tagcaaacat tccactggag tataatttca ctggctggct ggaaaactct
     1321 ccaatatcgg caaattccaa gatgtggggt atccgctctt ttatctgctt ttcggtataa
     1381 cccatcaaag tgccactaaa ataaatattt tctatgcctg tcaattcagg attaaaaccg
     1441 gctcctaact caagaagtgc cgccactttc ccattgactt cgaccgagcc agagctcgga
     1501 gttaaaactt ttgttaagat ctttaagagc gtcgatttgc cgctgccatt ttttccaatg
     1561 acccctaacg tctgtccttt gttaatttca aaacttatat tttttaaggc ctcaaagccc
     1621 cgatgatact gcttacgcag agggtgtaga gcctctttca cccgatccaa gggcttctta
     1681 taaagcttgt aatatttcga taaattttga actcgaaccg caaccttgga cgacataatt
     1741 ataacacatc cgcgaagtga gggcggagtc ttttaaaaac aaagaggcca aaaaatgaaa
     1801 atactaaaaa gaacagccag aaaaataagg tctgcaaggg aagttcccaa aaccaaactt
     1861 tcgatatcaa gctgtcacga taaccagaca ccacataatt cacaggattt aaaaatatga
     1921 taaacttaaa atcttcggga aggttttctg gtgaccaaaa aataggggtc acccagaaac
     1981 ctaattgcaa taacatcgta atcacttgcc ccaggtcacg atagaaaaca acgaccgcgc
     2041 tggtgagcca ggaaacggat aaaatcagta agcacagaca gaatgcatag tacggaagct
     2101 gcagccaata tttatccggc ccataaccat agaccaaaaa taaaaggagc atcacaagtg
     2161 ataaaaaacc aaaaagaatg gccgaggcga aaatttttat caaaggcaga agctcgacat
     2221 caaaaaccac ttttttaacc aaaaaggatt tctcgacaat gcaattgctg gcattttgaa
     2281 ccgcttcagc aaaaaaggtc cacggaaaca ttcccgtcac aagccataat ataaatggaa
     2341 gtccttttat aggcgatgac ttaaaccctt gttggaaggc aaaccacatt atgcctaaat
     2401 ttaacaaggg caataaaaag gcccataaaa tacccaaaac agatcccaaa tagcgactcc
     2461 gaaaatcact tttggctaaa ctccagataa ttcttctctg taaaataatg gtcttaagaa
     2521 actctctcat gtgtcatcaa cttaccgatt ccattaatat ctttctactt tatttcagct
     2581 taatgctttg cagcgctcta gcgatcgtag agataaccac ataaaaacat tgagattgca
     2641 aaagtaagag ggatttccta aattgtctga gtgaaaacaa ttcttgtagt atttggcaca
     2701 aggcctgaaa ccattaaact tgcacctttg attctccggg ccaaaaaaga ttctcatttc
     2761 aatgtcttgg tttgctctac gggacaacat cgcgagatga taaaacctct ttttaacttc
     2821 ttcggtatca aaccggattt tgacttagac ttaatgaagc ctggccagtc cttaactgat
     2881 atttccgtgg ggattatgcg ggagctctct gcgatatttg aaacccacac aattgatggc
     2941 cttgtcgttc aaggagacac cgcaagctgc ttcatcgcca gcctagtggc tttttaccat
     3001 cgcatcgaag tctttcatat tgaagccggc cttcgctcgg gggatctctt ctctccctac
     3061 cccgaggaat tcaatcgtaa aggcaccggc cttatcgcca aacttcattt tgccccgact
     3121 gaagccgcca aagccaatct cgttcacgaa cattatccag cttcggacat attcgtcaca
     3181 ggcaatacag ggatcgatgc actctttgag gcccagcgaa aaattcagga aagccctgag
     3241 ctaaaacttc agtttgataa gacctattct tttttaaatc cgcaaaagaa acttattcta
     3301 gtgacagttc atcgccgcga gtccttcgga gcccccatag aggagatcat gaaaggactg
     3361 gtggcgctgg cacaacgcga agacgtagaa atccttcttc ccttacatat gaatcctcag
     3421 gtgcgtaaat cagccgagca aattttcaac gggcatagct gttggatcga ccagaccaca
     3481 cctcctaaaa aagaccaaac tcggatatgg ctttgtgaac ctatcgatta tgtgcccttt
     3541 atctatttga tggaccgggc tcatctgatc cttaccgact cggggggcat ccaagaagaa
     3601 gccccttctc ttggtaagcc ggtcctcgtg gcccgtgaaa aaacagaacg cccggaagcg
     3661 attgctgccg gcacctccaa gctgattccc ctcgaagcaa gggctttttt aagcacggtc
     3721 gaagagcttc tggacacccc tgcgctttat gaccaaatgg caaaagcgca aaatcccttc
     3781 ggagacggac gggcttgtga aagaattttg aatattctgg ctgaaagatt ttaatgaaac
     3841 tgagccataa tttccttctc tttttacagt acgccgtatt tgctatgatc ctttttggga
     3901 tcaagctagc cttgatccat ttctatggga acgcaacccc ctattgggat cagtgggatg
     3961 ctgaagccgc caatctttat aaaccatttc ttgaggggac cctcacctgg aagagtcttt
     4021 tcactcccca caatgaacac catattctca taacaaagct gcttgcttta gctgaactga
     4081 aactcaacgg tctgtggaat ccattgctgc aaatggtggt caacgccgca ctccatattg
     4141 tggccatcct gtttattgtt gatcaattaa cccgggtcat tggccgaaaa tttttaacac
     4201 ccttgttaat tttttcattg gttctattta gtattcccta cgcctgggaa aacaccctgg
     4261 caggaataca gtctgtgttt tattttgtgt tattgtttag cacagcctgc ttatggctta
     4321 ccacgatcta ttctccctta tccttacgct ggtggtgtgg aatcagcttt gcggtgctcg
     4381 catttttttc cttggcttca gggattttcg ctctggccgc ggctgcaacc attggtctgt
     4441 ttttgtactt tactggattg caaaaaactc ctcagcaact ggtggccatt gtcattctca
     4501 gtgccctgtt tgccctcgga gccgccttga ctcccacctt agcctatcat gcgcctctca
     4561 aagccgcctc gccgctccaa tttttagatg ccttgaaaac catccttgct tggcctgcct
     4621 cttcaggttt tttagcggcc tttatccgca acttacccgc tttgcttttt gtgcgatcca
     4681 tgatgaaaac tcggccgtcg gcaaaggacc gaaggtggtt cttgttggcg ctgatcgttt
     4741 ggatgttggg gactgcggtg agcattgctt atggccgagc cggtggcagc accgcctccc
     4801 gctacctgga tttgttcgct atttttattc tgataaattt tgcctgctta atttttttga
     4861 gttacgaacg aataaaaaaa agacattttt ggactatgat gggcacggcc ctttggaccc
     4921 tcgcgatttc catcgccctt ttcttacctg ccatggagaa aattccgtca gagctgatgg
     4981 ataaacgtga cacgggttct tttcaagaaa taaacacacg aaaccattta ttaacaggtg
     5041 atgccaagga tctgcgcgat aagtcttttc ttcacgtgcc ctatccaaat tcagaacgtt
     5101 taatttcaat tttagcctcg ccttcagtcc gcgaaatatt gccccataac atcagaacac
     5161 ctttcactcc cacctcctct gacatcaaac ctgcggattc cctggtgaca aatggctatg
     5221 accccaaaac cccgggccgc acccatgtcc ttttaggcag ttacaataac caaggcaatg
     5281 acaatacagg aaaggcttcc atcaccttcg ctggcaaccc gacggcaacg gtgatggcca
     5341 ttcccatagc cggattccct ctagaaaaag gaatggagct ggctcttgag caaaatggaa
     5401 aacaaagccc tctggctatc actaaagatc cccaagaaag ctggagcata gcttatgcac
     5461 atattcaaga gggacccttt acccttcatc tgatggattc aagccccagt gcctgggtag
     5521 cgatcgacgc tccttcagtg gcagggagaa tggatgaact ggttttcatc ctcactgaaa
     5581 acttttatgt attcatctct agtggcattg cacttgcggc actcatagtg cttgctcaac
     5641 gatttcgatt cttcggcaaa tcataagtgt cagagaagat ctcattgtaa attttaaaca
     5701 tgtccttaat cgtagccaca atattcttca acgaagttga agcaccttga ccaaactgtc
     5761 ttgggaaagt ttgaattccc atttccccaa tacgatatcc ctttaaatca cttttgatgg
     5821 tcaattcagc tccgataaag ggagaattcg tacttaagat aagctcttgg acaagacttt
     5881 tacgcatggc tcttaagcca gtactaatat ctcggtaacg gattttaaat aagaaacgga
     5941 gaactatatt ataaacattt gaaataaatt gccgctttga agaataaagt tttttatatc
     6001 tgaaagtaat gatcagatca taaaaatctc tgattttgtg aatgcgtaaa aattcaaaga
     6061 catcgtactc atcgtcgcca tctgtaaaat agatccactc accgcgagcc tccgcaagac
     6121 cgcgtcgaat cgcggcgccg tagcctaaat tggtctcgtg atgaatgact cgaatctttg
     6181 gattcgcagc cgacaacgca tcggcgatca cccccgcgcg atccggcgag ccatcatcaa
     6241 taataaggat ctcccagttt tcagtgattt gggataacat tttgaccgat ttggcagtga
     6301 tgctctcgac agttccttcg tccttataga gtggaaaaaa aagagtgatg tctatttttt
     6361 ttgctacggt attatcagtc atttttgccc atcctttaaa agcgaagtct tttgtaaact
     6421 gcccaaggca actttgttaa gataaaaaga atgagattcc aaaaacttgg gaagacccga
     6481 agtccgaagt cacgatcttt caatttaaaa atgaattgag ccacagcctg cggcttcgcg
     6541 atgggaaaca agagggtttt tccgcgcgta aatcctgact ccatataacc cagcctcaca
     6601 acttgaatgt tggtgggaat ctgggcctga aaacaatagt gccgcaaaga aatagcgtag
     6661 ctttccaaag ctttttttgc tgcggcatag gaactattct gacttcgagg agccacggct
     6721 gcgatacttg aaataacgag aatagaacga caaaccgcct tttccctaag taaaagaatc
     6781 gcattcatga aaagacctat agcttcgaag ttcagccgca gcgaatgact gaaggcactt
     6841 aaagaaggga ccttttcgtc tccggtgaaa ctgccgcctg ccaccaaaaa ggcgtaatca
     6901 atactgccca gatcttcact caccttttca acgacccttt ctgcactcag tgctggatcc
     6961 agcaaatcgg tgggataaat aaatactcga acgccatgga cagtttgcaa ggaagattgc
     7021 agggcttgca ggtcctcttt gtcccgcgaa accaaggcaa gaccaaagcc tttttttgcc
     7081 agtaactccg ccaaagctct tcctaaacct gaagatccgc caactatcaa agcggtggaa
     7141 gttggtttca taagcccagc cttactctga gagcagaact gacagtttta gagggatcaa
     7201 aatcgaagat atctttttta aactgttgat aaccctcata ggtggccgcc accacgtctt
     7261 gcgtaagacg actgtcttta gcgatattag ataggcaatg gtggtcctca cagatctcat
     7321 caatttttcg aaaaagatcc agggctctgg ctgagttcgg aacattgagt gctaaacaaa
     7381 ttccatcggt taaaaagttg atataatttt gctgaccacg gaatattttt aaggagccca
     7441 aggagacagc gcctccaaat tccgcgatgg ccagggataa tttttcaatc actggcttta
     7501 attccgcatg cggaataatg atttgtgact ctaataaacc attttttcca aacaggcgat
     7561 gatatatttc tttaccgttt atgggaaagg caccagccag aatacctaaa ctctgtgatt
     7621 tcggtgcaaa ggattccttc atagaatacg cctgactgat tctatttttt aaggtccaag
     7681 cgaacacttg atccattaaa ctgcttcttg gtggagaata taaaacatta aactcctgga
     7741 tgtccggaac tgcctccgcc ccaaatttct cgtaataaac gtaacctgcg ccgaactttt
     7801 cacctttttg gttcaaatca ttccaggaat aaacctgttg gtattgatct ttttcattct
     7861 gcattaactc aatacactca aaaatattgg cacagggaag cgaagttctc acaagagatt
     7921 ttccagtcaa gggtttgcat tgaatggaaa ccgagaggat gtgaccggtc aatccgaagc
     7981 ctccggccgt cagctggaat ggcaaagatt ttggaaggca gagaattttt ccatgccttg
     8041 gatgaaacaa ctccatctct agaacagaat cggcgaaaag tccgctgtgg tattggcttt
     8101 tgccatgaac attggcagca acgcagccac cgagggtgat cgagggatga cctggaatcg
     8161 ctggcagcca ccagcccctc tgagaaagaa atttcaacag ttctccgacg gagagcccgc
     8221 tttccgcttt caccacacca gtttcattat tgaattcaag aattcggttg aactgcaagc
     8281 tcacaatcga gcgcacccca acaccagcac ttgccagcgg atagctcacc cccgctccgc
     8341 gagagataaa gggtcgctcc gacattgcga aatagagatc gcgatatttt tctgtgataa
     8401 acagctctcc ttcaacagaa aaagtagcgt caaaagagtg aattatttga ttttcagtca
     8461 ttagctggaa taatagccca aaatctcaac aaggtccaac gcagatgaat gaaaaaagag
     8521 ccgcttacac acagactcgc tacagtttgg atgcgaaaag aaccttagta tggaaagtct
     8581 ttgcgccttt cttacaaaag aagtttcaaa tccagggtca tgtattggat gttggttgcg
     8641 gcttcggtga tttcatcaat catatagagg ctcctcgaaa atccgcctta gatgctaacg
     8701 cagacatgca aaatttttta cagcctggga ttgatttcaa agatggtctc acttcagatc
     8761 tcaaaacttt ttttaaagcc aatgactttg actggatatt ctccagcaat ctgct
//
LOCUS       DATBTE010000238         7136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2217360, whole genome shotgun sequence.
ACCESSION   DATBTE010000238 DATBTE010000000
VERSION     DATBTE010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2217360"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..375)
                     /locus_tag="VIG33_08785"
     CDS             complement(121..375)
                     /locus_tag="VIG33_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270972.1"
                     /translation="MIAAQTLVGLACLLSKGGTVDQAQLQQQLTEPQKIHVVDLMSKD
                     PCLPENFEALIKQTEEKMNSNKGEIPEILAMPTVDCIKGE"
     gene            complement(399..1802)
                     /locus_tag="VIG33_08790"
     CDS             complement(399..1802)
                     /locus_tag="VIG33_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY8270973.1"
                     /translation="MKAKILLIEDDESFARSFSLILREQPVEVVWAADAKAGIQAYKA
                     APYSFATVVIDYVLPDLKGSEVCQHLRRLHPEQEFLFTSGHRDLDILTDILETGTSGF
                     LRKGRSPEETRERVLRSLEQYSKKTRIIGADNYSPSQAELELNMAGMTGCSKGLYQVV
                     KDVQNYKQSPYSTLIVGETGVGKELVAQALVPKGKNLITINCAAFRDKENLLESELFG
                     HVKGAFTGADKNTVGLVAQAHNNVLFLDELHHLSISAQAKLLRFLQEMKYRQVGDGSG
                     KETSVNFKLITAVQPDIKERVADGRFMQDLIRRVNDLVIQVPALNERPEDIEPLVRKF
                     QNEFNTDKPVEKRKQFRISTINEMMRHNWIGNIRTLKGAIRQMLTDCQSDIIEPKDFK
                     NYLLNKDVLSSNTAIMPNVESIEDAKNELESKIMKEALALGRTRSQAALRLGMALSTF
                     TRRLKELGINPELYLKT"
     gene            1985..3517
                     /locus_tag="VIG33_08795"
     CDS             1985..3517
                     /locus_tag="VIG33_08795"
                     /inference="COORDINATES: protein motif:HMM:NF033611.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAVED domain-containing protein"
                     /protein_id="HEY8270974.1"
                     /translation="MARGVVPLQQGFLYQAYYFWLEAARLFESHSKVSLVGYEINGAK
                     AFDDVHVQYNEPRRCGRGAKIEKDFFQLKFHVDHSGSVGYLDLIDPKFIGAKKVSFLQ
                     RLHELCKSNVDFAKNSCFHLYTPWVVNPNDPLSKIVNTDDGAIRIEKLQSAGGPKSEL
                     GKVKKAWMNHLQLISEDELYSTLIPLRIQQGPSLARLKESLNERLLHVGLTPIDFSKS
                     SDPYPGLIFRLCKEGRSQFTKEQLLEELQRDGLISAFHKRAAHTAKQMAIRSFFRSYE
                     HLEDVSELILSLELYFDGRFIKDPNMWNRELKAEISDFAKLNLVRNNSPLDLHLDVHS
                     SLAFATGSLISPKEAIDIAPVQKLRGQDLVWRVSNQPAPASKLTATPLSCGGGLEIAV
                     VLNITRSTTEEVKQFVAKSLQNVGVIYILEPETGVSQSAIKDADHAWAIAEQVSEHLR
                     ELRSKLNLNLPIHFFNSAPNSLLFFVGRLSAPAGHIFLYEYDFEKKRHGTYDVSVILP
                     WK"
     gene            3529..4668
                     /locus_tag="VIG33_08800"
     CDS             3529..4668
                     /locus_tag="VIG33_08800"
                     /inference="COORDINATES: protein motif:HMM:NF037736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270975.1"
                     /translation="MELTTYFEDFLAEIRPTGDDEEDYQNAHHKLRENLDEDADISKL
                     LVNTFLQGSYRRKTAVRVGASDKSDIDVVVVTKIPEEATPKRVVELFTPFLNKYYRGQ
                     WKTNNRSFEITLDNVKLDLVVTAAPTEADEEILKSEWASVASLPPEDKQKDLRLIKNW
                     IAESSTAQFKRDSLWQLMEKAGASEWKTEPLLIPDREMGEWDETHPLAQIEWTWEKNA
                     KCNTHYINVVKALKWWKKIHPSLRKYPKGYPLEHIIGYCLEDGTESVPKGVVNALERV
                     VSEFKTYYDSNTKPELKDHGVPEHDVFHKVSLEDFKNFYVYASEAAKTARAALDSNDK
                     GECIQLWQKLFGNEKFPGSDDTSGGRAGGYTPRTEASDPGKGRFA"
     gene            4671..6506
                     /locus_tag="VIG33_08805"
     CDS             4671..6506
                     /locus_tag="VIG33_08805"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEY8270976.1"
                     /translation="MTEKLPSSELRSGRRALDGIQECRLLSPLTWKVDHYKWHFKCRL
                     KSIGSALIPKESDWDVFIEDTYPWGDIKFFPSATNSIEVTFPHQSFNFPASKPSSWRL
                     GNPCLNTDVASLNRVDIDSEPTLAGARLLWQVRRLLKWLELAAAGSLLASGDPFEIPV
                     FPSSPKAGLSMVFDEGRESFDFWKDKYAQSGRCELFPYRKITPLSAKSNIYILWRHET
                     EDGKVKEVTWGNFLERRYNEKEVIVAPWILLSRVPTTVAWQTPITWQELSGICARDGI
                     NLFQIIADHLDELREQKQRVLLLGMPIPGRLGEENLQVHWQAIHIPSLSPSGSVPKGF
                     RPNRYGFHYRDIQDLAAIEKARCNWIISENWNKKEVTTRGVLDTQTHSFKTAVIGCGA
                     IGSYLAESLARNGLEHIMLIDTDSLVVGNLTRHTLGIDDVGFNKNNEVKNRINQSVPH
                     AKAEVMLGGFPPLEERSVKKLREVDIVLDCTASDKVLIDLARFDWGREKYFFSFSLGL
                     NGKRMFAFFAKATAFPSDKFRFDLKQWLIFEKSTLGSEFPREGTGCWHSVFPARNDDI
                     LIFCASAVKFIEECLNGNSTYIFTTFEQVNEAGKFSGIRRRDEPV"
     gene            6493..6984
                     /locus_tag="VIG33_08810"
     CDS             6493..6984
                     /locus_tag="VIG33_08810"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HEY8270977.1"
                     /translation="MNQCKERTFLSADKSYGVVLKSVALEVILVECRKSKKTETGGIL
                     IGKYSDDHNCALVYEISGPPRDSIRSATSFERGNQGLKDLLSGRFKDPGYYYLGEWHF
                     HPFSSPEPSGRDLSQIKDIATSALYKCPEPILLIIGGDPDEEWTVTANVFVAGNIIRL
                     QEG"
BASE COUNT         1952 a         1458 c         1714 g         2012 t
ORIGIN      
        1 tctgacctta agttctttta taggttgtaa cacgctcttc tcctttgtgg accaaaacgc
       61 aaaactcaag gcgcctgcaa tcaaagtggg cagaatccag tagagctttt tgtccatgta
      121 ctactcgcct ttgatgcaat caactgtcgg cattgccaga atttctggaa tttcaccttt
      181 attagaattc attttttctt cggtttgctt gatgagagct tcaaagtttt caggcagaca
      241 ggggtcttta gacattaaat ccacgacatg tatcttttgg ggctctgtga gttgttgttg
      301 gagttgggcc tggtctacag tgccaccttt tgaaagtaga caagcaagtc ccactaatgt
      361 ttgtgctgca atcataaaat tttcctccgt ttaagcgttt aggtttttaa gtacagttca
      421 gggttgattc caagttcttt caaacgtcga gtaaatgtag acaaagccat gcccagccgt
      481 agggcagctt gagaacgggt cctgccaagt gctagagctt ctttcatgat ttttgattcg
      541 agttcatttt ttgcatcttc aatagattct acattgggca tgatcgcggt gtttgaactc
      601 aaaacgtctt tattaagaag atagttttta aagtctttgg gctcaataat gtcagactga
      661 caatcggtga gcatttgtct gatggctccc ttaagtgtgc gaatattgcc aatccagttg
      721 tgccgcatca tctcgttgat ggtagagata cggaattgtt ttcttttttc cacaggcttg
      781 tcagtgttga attcattttg gaactttcga accaggggtt caatgtcctc tggccgttca
      841 ttcaaagccg ggacctgaat gaccaaatca ttcacacgac gaattaagtc ttgcatgaat
      901 ctaccatcgg caacccgttc tttaatatca ggttgtacgg cagtgatgag tttgaaattg
      961 acggaggttt ccttgccgct accatctccg acttgacggt atttcatttc ctgtaaaaag
     1021 cgaagtaact tcgcttgagc ggatatcgaa aggtgatgga gttcatcaag aaacaagaca
     1081 ttgttgtgag cttgtgcgac caaaccgaca gtgtttttgt cagctcccgt aaaggcgccc
     1141 ttaacgtggc cgaaaagttc cgactccaag agattctctt tgtctcgaaa ggcggcgcag
     1201 ttaatggtga tcaggttttt tccttttggc acaagggcct gcgcgaccag ctctttacca
     1261 acgccagttt cgccgacaat cagtgtggag tacggagact gcttgtagtt ttgaacgtct
     1321 ttcacgactt ggtagaggcc tttcgaacaa cctgtcattc cagccatgtt caattcaagc
     1381 tcggcctgac ttggcgaata gttatcggct ccgatgattc gggtcttttt ggagtattgt
     1441 tctagtgagc gtagaaccct ttctcgagtc tcttctgggg agcggccctt acgaagaaag
     1501 ccgctggtcc cagtttcaag gatatcggtt aaaatatcga ggtctcgatg tccgctcgta
     1561 aaaaggaact cttgttctgg gtgcagcctg cggagatgct ggcagacctc gctcccttta
     1621 agatcgggta ggacatagtc aatgacaacg gtggcaaagc tatagggagc ggctttatag
     1681 gcctggatac cagcttttgc gtcggcagcc catacaactt cgacaggctg ttctcgcaat
     1741 attagcgaga aggaccgggc aaaggattca tcatcttcga tcagtagaat cttagctttc
     1801 ataagttctc cgcgcattga ctttaagcgt tatatcaaat ccagtttaat atttccatgg
     1861 agaagggaaa aaaagtgttt aaagtgacct tgtttaaatt ggctggaaaa aaatacagag
     1921 gttaaggtcg gaacgagttg ggttttagta gtatggggca ttacattttg acctaggagt
     1981 atcagtggca cgtggggttg ttcctctgca gcaaggattt ctttatcaag catattattt
     2041 ctggcttgag gcagctcgct tgtttgaaag tcattctaag gtgagcctag ttgggtatga
     2101 gatcaatggc gcaaaagctt ttgatgatgt gcacgttcaa tataatgaac cgcgcagatg
     2161 tggccgaggg gcaaaaatcg aaaaggactt ttttcagctc aaatttcatg tcgatcacag
     2221 tggaagtgtc ggctatttgg acttgatcga tccaaaattt attggagcaa agaaagtctc
     2281 ttttctgcaa cggctgcatg agctgtgtaa gtctaacgtc gattttgcga aaaatagttg
     2341 ctttcacctc tacactcctt gggtggtgaa tccaaatgat ccgctttcga agatcgtcaa
     2401 tactgacgat ggtgcgattc gtatcgaaaa actccaatct gcaggtggcc caaagtctga
     2461 acttggtaag gtaaaaaagg cttggatgaa tcatctccag ctaataagtg aagacgagtt
     2521 gtattctaca ctaattcccc tacggattca gcaaggacct agtcttgcga gattgaaaga
     2581 gtctttgaac gaaaggctct tgcatgttgg gctaacgccg attgatttta gtaaatcgag
     2641 tgatccctat ccaggtttaa tttttagact ttgcaaggaa ggccgtagtc agttcaccaa
     2701 agaacagcta ttggaagaac ttcaaaggga tggattgatc tctgcgtttc ataaacgagc
     2761 agcacatacc gctaagcaaa tggctattcg aagtttcttt cgaagttatg aacacctcga
     2821 agatgtttcg gaattaattc tttctcttga actctacttt gacgggcgct tcataaaaga
     2881 tccgaacatg tggaatcgag aactgaaagc ggagatctcg gatttcgcga agttaaatct
     2941 tgtaagaaat aattcacctt tagatcttca tcttgacgtt cacagttccc ttgccttcgc
     3001 aacgggctct ctcatctctc caaaggaagc gattgacatt gctcctgtcc aaaaactgcg
     3061 gggacaggat cttgtgtgga gagtatccaa ccaacctgct ccggcgtcta agcttacggc
     3121 aactccttta agttgtggtg gtggtttgga aattgctgtc gttttgaaca ttactcggtc
     3181 gacgaccgaa gaagtaaaac agtttgtggc taagtccctt cagaacgttg gagtgattta
     3241 catccttgaa ccagaaacgg gagtgagcca gagcgcgatc aaagatgctg atcacgcctg
     3301 ggcgattgct gagcaggtca gtgaacattt aagagagctg cgttcaaaat taaatttgaa
     3361 tcttccgatt cattttttta actctgcacc gaactcattg ctcttctttg ttggcagact
     3421 ttcggcgcct gcgggacaca tttttttgta tgagtatgat tttgaaaaga aacgccacgg
     3481 aacctatgac gtttccgtaa ttttaccctg gaagtgagaa ctaaaaatat ggaactgacc
     3541 acttatttcg aagatttcct agccgaaatc aggccaactg gtgacgatga ggaggattac
     3601 caaaatgccc atcataagct gcgtgaaaac ttagatgaag atgctgatat ctcaaaactc
     3661 ctagtgaata cttttcttca aggcagttat cgtcgaaaaa ctgccgtcag agtgggtgct
     3721 agtgacaaat cggacatcga tgttgtggtg gtaactaaaa ttccagaaga agcaactccg
     3781 aaaagggttg tcgaactctt cacgcccttt ctgaataagt attatcgagg gcaatggaag
     3841 accaacaacc gctccttcga aatcacattg gataatgtga agttggacct cgttgtcacc
     3901 gccgccccca ctgaagcaga tgaagagatc ctaaaatccg aatgggcctc tgttgctagt
     3961 cttccaccag aagataaaca aaaagacttg aggcttatca aaaattggat tgcggaaagc
     4021 tcgaccgcac agtttaagcg cgacagtttg tggcagctca tggaaaaagc aggggcgtct
     4081 gaatggaaaa cggaaccgtt gcttattcca gatcgtgaga tgggtgagtg ggatgagact
     4141 caccccctag cccaaatcga atggacttgg gagaaaaatg cgaagtgcaa cacacattac
     4201 atcaacgtag tgaaagctct taaatggtgg aagaagatcc atccctcact tcgtaagtat
     4261 ccaaagggct atcctctgga acatattatc ggctattgct tagaagacgg gactgagtcg
     4321 gttccaaagg gcgtcgtaaa cgctcttgaa cgcgtcgtat ccgaattcaa aacctattat
     4381 gattctaaca ccaagcccga gctgaaagat catggggtgc ccgaacatga tgtctttcac
     4441 aaagtgtcac ttgaagattt caaaaatttc tatgtctatg cgtcggaagc tgcgaagact
     4501 gctcgagcgg ctttagattc gaatgacaaa ggggaatgta ttcagttgtg gcagaagctt
     4561 tttggaaatg agaaatttcc aggctctgat gatacaagcg gaggccgggc tggaggctac
     4621 actccgcgta ctgaagcgag cgacccaggt aagggacgct ttgcctaacg atgactgaga
     4681 aattgccttc aagtgaatta agaagcggac ggcgtgccct tgatggaatt caagagtgtc
     4741 gtttactatc gccgctcacg tggaaggttg accattacaa atggcatttc aagtgtcgac
     4801 tcaaaagcat agggtcggcg ctcattccaa aagagtcaga ttgggatgta tttatcgaag
     4861 atacttaccc ttggggagac attaaatttt tcccgagtgc gacgaattca attgaagtta
     4921 catttcctca tcagtcattc aacttcccag caagtaagcc ttcgtcatgg aggctcggga
     4981 atccttgcct caacactgat gtggcctcat tgaatcgagt agacattgac tctgaaccaa
     5041 ctttggccgg agcacggctt ttatggcaag ttcgacgcct attaaagtgg ctagagcttg
     5101 ccgccgctgg ttctctactc gcctcgggag atccgttcga gattccggtt ttcccgtcta
     5161 gtccgaaggc gggtttgagt atggtgtttg atgaaggccg tgaaagcttt gatttttgga
     5221 aagataaata tgcgcaatca ggccgttgcg aactctttcc gtatcgcaaa ataactccgc
     5281 tatctgcaaa atcaaatatt tatattctct ggagacacga aacagaagat ggcaaagtaa
     5341 aggaagttac ctgggggaac tttcttgaac gtcgttacaa tgaaaaagaa gttattgttg
     5401 ccccttggat cttactttct cgtgtgccaa ccacagtagc ctggcaaacg ccaatcacct
     5461 ggcaggaatt aagtggtatt tgcgcgcgtg atgggattaa tctttttcaa atcatagctg
     5521 atcatcttga tgaacttcga gaacaaaaac aaagggttct gttgctggga atgccgatac
     5581 cagggaggtt gggtgaagaa aatttacaag ttcattggca agcgatccat ataccctctc
     5641 tgagtcccag cggaagtgta cctaaaggat ttcgcccaaa cagatacggt tttcattata
     5701 gagatattca agacttggcc gcgattgaga aggctcgttg caattggata atatcggaaa
     5761 actggaataa gaaagaagtc accacgcgcg gtgtgcttga tacccaaacg cattcgttta
     5821 agactgccgt cattggatgt ggtgcgattg gatcatatct tgcggaaagt cttgctcgaa
     5881 atggtcttga gcacattatg ttgattgata cagattcttt agttgtaggc aatttgacca
     5941 gacacactct tggtatagat gacgtcggtt ttaataaaaa taatgaggtt aaaaatcgta
     6001 ttaatcaatc ggtgcctcat gctaaggccg aagtcatgct gggcggattt ccgccactcg
     6061 aagaacgcag cgtcaagaag cttcgtgaag tagatattgt cttggactgc actgcttccg
     6121 ataaagtact cattgatttg gcgcgatttg attggggaag agaaaagtac ttcttttcat
     6181 tttctctagg cctaaatgga aaacggatgt ttgcgttttt cgcaaaagcg acagcatttc
     6241 cgtctgataa atttaggttt gacttgaagc aatggttgat attcgagaag agcaccttag
     6301 gaagtgaatt tccgagggag ggaacgggtt gctggcattc agttttcccc gcccgcaacg
     6361 atgatatatt gattttttgt gcttcagccg tgaagttcat cgaggagtgt ttgaatggaa
     6421 attcaacata catttttaca acttttgaac aggttaatga agctgggaag ttctcgggga
     6481 ttcggaggag agatgaacca gtgtaaagag cgaacatttt tgtcagcaga taaaagttat
     6541 ggagttgtcc ttaaaagtgt tgctctcgaa gttattttgg ttgaatgtag aaaatcaaag
     6601 aaaactgaaa cgggtggcat tctcatcggt aaatactctg atgatcataa ttgcgcttta
     6661 gtctatgaaa ttagcggacc accgcgagat tcaattcggt cagcaacgtc atttgagcgc
     6721 ggcaatcaag gccttaagga tttactgagt gggcgtttta aagatcccgg ttactactat
     6781 cttggcgaat ggcattttca tcccttctca agccccgagc caagcggaag agatttatct
     6841 caaatcaaag atattgccac ctctgcccta tataagtgcc ctgaaccaat cttattaatt
     6901 attggcggag atccggatga agaatggact gtaaccgcga atgtgtttgt ggcaggcaat
     6961 ataattagat tacaggaggg ctgactgcct tctttataac ccccgaaatt ttctccagtg
     7021 caactcaggc aataacaagc tgagttgaag gtagccacct gacggttcat ttgagactca
     7081 gtttttgcga aaaaatggag ttaaacaaat gagttaccgt cgggtgacca gtgagg
//
LOCUS       DATBTE010000239        13055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_508625, whole genome shotgun sequence.
ACCESSION   DATBTE010000239 DATBTE010000000
VERSION     DATBTE010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 13055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13055
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508625"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VIG33_08815"
     CDS             complement(<1..412)
                     /locus_tag="VIG33_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270978.1"
                     /translation="MLKVGQLIRFVAGLNPQNTPAKLSFYNFLRGFPHPDEALTPELI
                     ELFFTYCMDYPHWAANKNQLSNEVQYLLENFNNFYQQHIDLNKIRFPQTMQLLEIERM
                     PDLEEILDIYLKRQYSESDKFRLINDQNKRVIAII"
     gene            492..998
                     /locus_tag="VIG33_08820"
     CDS             492..998
                     /locus_tag="VIG33_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270979.1"
                     /translation="MQKELNPELFGEKKSTSPARREALVPPAPSTDPVYLNTDRQIFE
                     LKSQMMSVNDQLSKLNQALNETNKNGSARMERFQQGLNRLETSHNGLATEAGQRIQQI
                     NNKLAERKALDLKIQEMVDRHNNVIKSFEVRLNHMQKLLTEKEAQTAAAYTALNEAKM
                     EIARLKRL"
     gene            complement(995..2824)
                     /locus_tag="VIG33_08825"
     CDS             complement(995..2824)
                     /locus_tag="VIG33_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEY8270980.1"
                     /translation="MKKSIFYFLLSAVFLLDFWPRTFVQAQGPEENPDPLLTEVSLSL
                     YEWNPGQSGDLQIKLKLPKGYHAYEDMFRLTLLEPDGFKISGFKLKPVHEFYDKFTKK
                     SRRGVQESGTMTAHLEAPLKFQKASSHLVFELTYQACSDSFCLFPVTKKIETPCKLIG
                     APQEALLQSPSASSTPTVASKSLFSADYFAEMLNKGGFVIFVLAFFAGILTSFTPCIF
                     PMIPITLAILGDHSDKRSRRENFLISCIYVLGIATTYSTLGLVAASSGTLFGASLGNP
                     YVLSLVCFVFLAMSLSMYGLYDLQMPVWVRQKFGGKVATGNRHLTIYLTGLFAGIVAS
                     PCVGPVLVTILAYVATHQSKILGFFLLFTYALGLGLIFIVLGLSNQFTRLLPRSGIWM
                     EGVKFVLGSLMLSAFYYYLSLLVPLRAFEVALGIGLIAVASVFGAFKLAKNPSQGLRK
                     GLMIALLLIGCGYLSFGIFNLQALMKSQSNQKFISETGVNKTQQLNWQPYSEELLQKA
                     VQEKKPVIIDFWAEWCAACHELEQFTFTDAKIHALSEQFVLLKFDATKDSDQLRYLKK
                     KYKIQGLPTVLFYNPHGVWLEALTLINYENASKFLSRMDKAKN"
     gene            2957..4948
                     /locus_tag="VIG33_08830"
     CDS             2957..4948
                     /locus_tag="VIG33_08830"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY8270981.1"
                     /translation="MADENDFLWLEELYSEKSLQWVRSQNEKTVKAFKQHPKFHQFFE
                     EASLLHSSDEALPFRSVNNGYVYHLEVSAKNPLGRWVRVSLKDFTQENIPWELLLDID
                     ELSKTEQRQWYYAGAFFSPRGEKCLLSLSDRGHDRSIKREFDLSAKKFVTEGFVIPES
                     KSNAAWFDEDTLLVGGYEESGINHLGYPRVVKVWRRGQELSKAEMLFEGKFEHYGVWA
                     GDLEEKKCLIHEGVSWDEGKTYLYDHSRKLKQLHLPLEARAIGLFDQDVIFNLHKDFK
                     GYKAGSVLQVSLEKAFNNSHQDQFKVIAQDTKESFIDGIEILKDSFLTFTLKNVSHEL
                     WHYKKESDTWKAERLQIPEQSTVTAVLEDAVTKQVFVVSENFLTPKAVSQLKADNSLL
                     FLKKAKDIFTGNFTVQQNWAKSTDGTSIPYFVVHKKDLKLDSTHPTLLNAYGGFTISK
                     LPMYMPAPGKLWLEKGGVFVLANIRGGAEFGMNWHRSAILENKQKSYDDFIAVAEDLI
                     SRKYTTSERLGIRGGSNGGLLMGAVTTQRPDLFKAVLCEVPLLDMIRYVELPPGASWM
                     DEYGDPRNEKMREVILKYSPYQNVHKEKKYPKIFFHTSITDDRVHPSHARRMAAKMLE
                     QGHELFFYEESEGGHGSGDLKTKAFAEAMSFSYLWMMLG"
     gene            5023..5889
                     /gene="motA"
                     /locus_tag="VIG33_08835"
     CDS             5023..5889
                     /gene="motA"
                     /locus_tag="VIG33_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEY8270982.1"
                     /translation="MGFVGILIVFATVFGGYIAGGGKMHVIIEAAILEMVIIGGAAFG
                     AYVVANPMKIIKMGIKLAIKAMTAKGPQKKEYVELCQMMFQLFQVLRKEGPQGIEKHI
                     ENPEQSEIFKAYPSFMHNHHAVHFLCDTMKITLSAELSQYDVDDLLSADIKGIHAEEH
                     LAQHAVMAVAGGFPGLGIVAAVLGIVKTMGHLTEGTEVIGEHVAHALVGTMLGVFCAY
                     GLIEPTGTKMAADIEAEGRYLEVIKAAMIACQRGAPPIVCVEFGRRSIMPEERPSFDE
                     IDKATKEIKKAA"
     gene            5896..6906
                     /locus_tag="VIG33_08840"
     CDS             5896..6906
                     /locus_tag="VIG33_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEY8270983.1"
                     /translation="MAEKKTTIVIKKVTIVAGGGHGGSWKVALADFMTALMAFFLVMW
                     LLGQSDETKKAVSDYFSTPSVIEYNFQNFGVEITLEKLFLDLINEPLKAFQQFIEPVA
                     KTPNLLDMGSTKVVTAFLADQMNDYAKNVQVSAEGFDFDIPDTVLFERGTSNPLSTFV
                     DVMDKIKGVTTGLQEAEIKVTSGLFVQSVPGSNVALANKVSAQRLDLVKNKILAGLEF
                     NTVTLQSYSSVKEKRGEVDPQKLIGFIRLSIRQKDVTSTGKKPRKLETVFGDPNVSMS
                     VYDNFVKQAAERKSHEEPAPKKFGKVKKIENPVNEEMKDHNELNDRNDEVTPLPEAPT
                     EE"
     gene            7040..7312
                     /locus_tag="VIG33_08845"
     CDS             7040..7312
                     /locus_tag="VIG33_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270984.1"
                     /translation="MRGYKENQQEDMFSYVSMEDAIPKKHPLRKIMAMVDSLLDEMWS
                     DFDGLYSGVGRPSIPPEQILRNYLKTIIPLQFYKVVCTTEGRRRAE"
     gene            complement(7251..8105)
                     /locus_tag="VIG33_08850"
                     /pseudo
     CDS             complement(7251..8105)
                     /locus_tag="VIG33_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            8388..8711
                     /locus_tag="VIG33_08855"
     CDS             8388..8711
                     /locus_tag="VIG33_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270985.1"
                     /translation="MKKVVLSALIMLASSAAFADSSSGTVSALHVHEHFSMFDIKGET
                     AQKIYNGLQTQEKEDGNAAGQKMGSLSKVSADGRMWCQKDQGIYKCQVTFDTTTGSIN
                     QTWLK"
     gene            complement(8881..9201)
                     /locus_tag="VIG33_08860"
     CDS             complement(8881..9201)
                     /locus_tag="VIG33_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270986.1"
                     /translation="MNELKCWYRRHRGKNVVLFEDETYLLLFPPLRSAWARRGKPNRF
                     WLSGHNAKKVIFSSINLKTGHHIEMEQPDPLPAGRSALTDSFAIPFSSAAVYFLKAKF
                     QISF"
     gene            9373..9591
                     /locus_tag="VIG33_08865"
     CDS             9373..9591
                     /locus_tag="VIG33_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270987.1"
                     /translation="MIGLNEGSTLGADKGYDTADHGQDLSQLNIKSHVAQNIHARKKT
                     SSIDGRTTSHSGYEVSQRKRMCIEEIFG"
     gene            10230..11318
                     /locus_tag="VIG33_08870"
     CDS             10230..11318
                     /locus_tag="VIG33_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEY8270988.1"
                     /translation="MDSNHSDNLNTFEEKLKTLEDQALIEQLQKLFERKKRIGDAILL
                     NLQEIADRRLYAKMGYSSIFEFLVKHFHLSESAAYQRTNALKLIREVPKAQEALFKGE
                     TNLSTMAATQSFIKQLENEQEAPLTKAEKETIFDAVKGKSKKEAQATFAEMNPVATLP
                     ETKAKVLTAKHTLLQVTLEQETLDLINEIKNLLSHEIPDGHLNKVLKRMAEISVGQLK
                     KKKGRSISNENSNSKKERTKNTVEKEKANPSPILSEETECSLSRSQSPSDSNCPNENH
                     RSSKQRASKSRHIPIEIRSQVFQRAHGGCEYVSPDEQRCQNHYQLEVDHITSWSQGGE
                     TSLENCRVHCKSHNQYRVKETHGFWYEH"
     gene            complement(11442..12161)
                     /locus_tag="VIG33_08875"
     CDS             complement(11442..12161)
                     /locus_tag="VIG33_08875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEY8270989.1"
                     /translation="MKYRCFYKLTLKLSLLFTSLLLCMPCLAQQEAKSEVPTFKMNGE
                     ISLLSNYVKHGLTQTNKDPSLQGAFGFNFGPQFKLGLWGSNVNYDSTEHFLLKINAEL
                     RVQVSTITDFKLGVFDNHYFKTSTRDGNTTYLAITSQEYRVRYESETNWEGTDESSTY
                     FSFGKTTELSPSWKWDNEIGYTMVSAANLTNYFDLRTSFIYKGGNNLLYQITGTTTSA
                     PTQFSNGQGDVAILIGASTSF"
     gene            12225..13055
                     /locus_tag="VIG33_08880"
     CDS             12225..13055
                     /locus_tag="VIG33_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEY8270990.1"
                     /translation="METHRPWTEYTFVAFDTETSGAYPIGSDIIEFGAVKWRGGQEIA
                     SFQTLLKPRELLTEFNMGIHGISNEMVANAPLISEKIREIYEFMEDSVTMAHHAPFDM
                     GFVTVELETHRLPLLMSPALCTSLLSRKWIHGTENHKLQTLVKFLNIEGGAAHRALDD
                     ARACLQVGLECFKRMPADVTLTEAMKSQGKKLQWPDYSLLRWNNPLVKVLIEAIYQKK
                     NAEILYANSSASSSEPRQVTPMGIVRNPDGDYLQAFCLRDKTSKRYYLAKIKDAALVY
                     "
BASE COUNT         3825 a         2682 c         3090 g         3458 t
ORIGIN      
        1 gaatgatggc aatgacacgt ttgttttgat cgttgatcaa acggaattta tcgctctctg
       61 agtactgacg tttcaggtag atgtctaaaa tctcttccag atccggcatg cgttcaattt
      121 ccaaaagctg catggtctgg ggaaagcgga ttttattcag atcaatatgt tgctgataga
      181 agttgttgaa gttttcaagc aaatactgga cctcgttgct caactggttc ttattggccg
      241 cccagtgagg atagtccatg cagtaggtga agaacaattc gatcaattcg ggagtgagag
      301 cctcatcagg atggggaaaa ccccgcagaa agttgtaaaa agacagcttc gcaggagtat
      361 tctgagggtt aagaccagcc acaaatctaa tgagttgccc aacttttagc atgttacctt
      421 tttatgaaga attgatttta gtgtccagaa gaaatagcat ggaactcgta gcaaattctc
      481 taggaggagt catgcaaaag gaattaaatc ccgaactttt cggtgaaaag aaaagcacat
      541 cacccgctcg tagagaggcc ttggtcccac ctgctcctag tacggaccct gtttatctca
      601 atacagatcg ccaaatcttc gaacttaaaa gtcaaatgat gagtgtgaat gaccagttaa
      661 gcaagctcaa tcaggcgctg aacgagacga acaaaaacgg cagtgcccgt atggagcgct
      721 ttcagcaagg cctcaatcgt cttgagacca gtcacaatgg tctagctaca gaggcgggac
      781 agagaattca gcaaataaac aataaactgg ccgaacgaaa agcccttgat ttgaaaattc
      841 aagaaatggt ggatcgccat aataacgtga tcaagagttt tgaagttcgc ttgaatcaca
      901 tgcaaaagct tttgaccgaa aaggaagcgc aaaccgcggc ggcttatacg gcacttaacg
      961 aagccaaaat ggaaatcgcg cgtttaaaac gtctttagtt tttagcttta tccatcctgc
     1021 ttaagaattt agaagcattc tcataattta tgagggtgag agcttcaagc caaactccgt
     1081 gaggattata gaagagaacc gtcggtaacc cttgaatctt gtatttcttt ttgagataac
     1141 gcagttggtc agaatcttta gtggcatcga attttaataa aacaaattgc tccgagagag
     1201 cgtgaatttt tgcatctgta aaggtgaatt gctcgagctc gtggcaagcg gcacaccatt
     1261 ctgcccaaaa atcaataata acaggctttt tctcttgaac ggctttttgc aggagttctt
     1321 cactgtatgg ttgccaattt aactgctggg ttttattcac tccggtttca ctgataaatt
     1381 tttggttgga ttgtgacttc atcagcgctt gcagattgaa aataccaaag gataaataac
     1441 cacatccaat caataaaaga gcgatcatca agccttttcg caggccttgg ctaggatttt
     1501 tagccaactt aaaggcgcca aaaacactgg ccaccgcgat caaaccaata cccaaagcga
     1561 cttcgaaggc gcgaagagga accaggagac ttaggtaata gtaaaatgcg ctcagcataa
     1621 gagagcccag cacgaatttg actccctcca tccaaattcc tgaacggggc aagaggcgag
     1681 tgaattgatt gctgagacct agaactatga aaatcaaacc tagacctaag gcataagtaa
     1741 agagcaagaa aaagccgagg attttgcttt gatgagtagc cacataggcc aggatcgtta
     1801 ccaggactgg gccgacgcag ggactggcta cgatgccagc aaagaggccg gttaaataaa
     1861 ttgtaagatg gcggttgcca gtagcgactt tgccgccgaa tttttggcga acccacaccg
     1921 gcatctgcag atcataaagc ccatacatcg ataaggacat ggctaaaaac acaaaacaaa
     1981 caagcgaaag cacgtaggga ttgccgaggc ttgcgccaaa caatgtgcca ctggacgcgg
     2041 cgacgaggcc caaagtggag taagtggtcg caattcctaa cacataaata caactaataa
     2101 gaaaattctc acgccggctg cgtttatcag aatggtcccc taagatagcc agggtgatcg
     2161 gaatcatcgg aaaaatacag ggtgtgaaac tggtaagaat tcccgcaaaa aaggcgagca
     2221 cgaaaataac aaagccccct ttatttaaca tttcggcaaa gtaatctgcc gagaagaggc
     2281 ttttgctggc gaccgttggc gtcgaggaag cggaagggct ctgcaagagg gcttcttgag
     2341 gggcgccgat cagtttgcag ggagtttcaa ttttttttgt cactgggaaa aggcagaaac
     2401 tatcggagca agcttggtaa gtgagctcaa acaccaaatg ggaggaagcc ttttgaaatt
     2461 tcaacggtgc ttctaaatga gccgtcatag tgccggattc ttgtactccg cggcgacttt
     2521 ttttagtgaa tttatcataa aactcgtgga ctggctttag cttaaacccg ctgattttaa
     2581 aaccatcagg ctcaagcaag gtgaggcgga acatgtcctc ataggcatga taacctttgg
     2641 gcagcttcaa ttttatttgc agatcgccac tttgtcctgg attccattcg tagaggctca
     2701 gtgagacttc agtcagaaga ggatcggggt tttcctcagg tccttgcgct tggacgaagg
     2761 tccttggcca aaaatcaaga aggaacaccg cgctcaatag aaaataaaaa atggattttt
     2821 tcatggtgtg tctactttat cttaatgctt ctaatcaagt ctatgaaatc acacaaaagt
     2881 tcttggcaaa atcaaaatca gctctaggat actgtttttg gggcattaac cttatgtcga
     2941 ctaaagtggg tgatccatgg ctgatgaaaa tgactttttg tggctagaag agctttattc
     3001 tgaaaaatca ttgcagtggg ttcgcagtca aaatgaaaaa acagtaaagg cttttaaaca
     3061 acatccaaag tttcatcaat tttttgaaga agcgagctta ctgcattcca gtgacgaagc
     3121 tttgccattt cgctctgtca ataatggcta tgtttatcat ttggaggtca gcgcgaaaaa
     3181 tccgctggga cgctgggttc gagtctctct taaagacttc actcaagaaa atattccttg
     3241 ggaattgctt ctggatatag atgaattgtc gaagacagaa cagcgccagt ggtattatgc
     3301 gggagctttc ttttctccga ggggggaaaa atgcctcctg tcgctttccg atcgcggaca
     3361 tgatcgcagt atcaagcgtg agtttgatct gtcggcgaaa aagtttgtga ctgagggttt
     3421 tgtaattcct gaatcgaagt ccaatgcggc atggtttgat gaggatactt tacttgtcgg
     3481 tggatatgag gaaagcggca tcaatcattt aggatacccg cgtgtggtga aggtatggag
     3541 acgcgggcag gaattgtcca aagcggaaat gttattcgaa ggaaaatttg aacattacgg
     3601 agtttgggcc ggagaccttg aagaaaaaaa atgccttatt catgaaggag tcagttggga
     3661 tgaagggaaa acctatcttt acgatcattc tcggaaattg aaacagcttc acttgccgct
     3721 ggaagcgcgg gcgataggcc tttttgatca agacgtcatt tttaacctac acaaagactt
     3781 taaaggatac aaagcgggga gtgtgctgca agtttctttg gagaaagcat ttaacaactc
     3841 ccaccaagat caattcaagg tgattgcaca agataccaaa gagtccttta tagatgggat
     3901 cgagatttta aaagattcat ttttgacatt cactcttaag aatgtcagcc acgagctttg
     3961 gcattataaa aaagaaagcg atacttggaa ggccgagaga cttcaaattc ccgaacagtc
     4021 tacggtgacc gctgttttag aagacgcggt gacgaaacag gttttcgttg tgagtgaaaa
     4081 ctttttgact cctaaagcgg tctctcaatt aaaagcggac aactccctgc tgtttttaaa
     4141 gaaagccaaa gacattttca caggcaactt cacagttcag caaaactggg caaagtctac
     4201 ggatggcact tcgattcctt attttgtggt gcataagaaa gatttgaaat tggattcaac
     4261 tcatcccact ttattaaacg cttacggagg atttaccatt tcaaaattgc cgatgtatat
     4321 gccagctcct ggaaaattat ggcttgaaaa gggcggcgtt tttgttttgg ccaatatccg
     4381 tggtggggct gagttcggaa tgaactggca tcgcagcgct attttagaga acaaacaaaa
     4441 aagttatgat gatttcatcg ccgtggccga agatttaatt tctagaaaat atacgaccag
     4501 cgaaagattg ggaattcgtg gcggtagcaa tggcggtctt ttaatgggtg ctgtgaccac
     4561 gcaaagacct gatttgttta aggctgttct atgtgaagtg cctttattgg atatgatccg
     4621 ctatgtggag ctgcctccgg gggcctcgtg gatggatgag tacggcgatc cccgaaatga
     4681 aaaaatgcga gaggttattt taaagtattc tccttatcaa aatgtgcaca aagagaaaaa
     4741 atatcctaaa atatttttcc atacttccat taccgacgat cgggttcatc caagtcatgc
     4801 ccggcgtatg gctgcgaaga tgctggagca agggcatgaa ctctttttct atgaagaatc
     4861 agagggcggc catgggagcg gggatttaaa aacaaaagct ttcgcggaag ccatgagttt
     4921 tagttaccta tggatgatgc taggctagac tatagacgag tttatcctta ggtctctaaa
     4981 tagcataagc cactctgccg ataaagcctc aagaggtaaa tcatgggatt tgtcggcata
     5041 ctgatagttt tcgcaacagt cttcggtggg tatatcgccg gaggcggtaa aatgcacgtc
     5101 attatcgaag cagcaatcct cgagatggtc atcattggtg gtgccgcgtt tggggcctac
     5161 gttgttgcca accccatgaa gattatcaag atggggatta agcttgcaat taaagcgatg
     5221 accgcaaagg gtcctcagaa gaaagagtac gttgagcttt gccaaatgat gttccagctt
     5281 ttccaagtgt taagaaaaga aggtccgcaa gggattgaga agcacattga gaatcctgag
     5341 cagagtgaga tttttaaggc ttatccaagt tttatgcaca atcatcatgc cgtgcacttt
     5401 ctctgtgaca cgatgaaaat cactttatca gcggagcttt ctcaatatga tgtcgatgat
     5461 ctcttgagcg cggatatcaa aggtattcat gccgaagaac atttagcgca acatgcggtg
     5521 atggcggtgg ccggaggatt tccgggacta gggattgtgg ccgcggtttt gggtatcgtg
     5581 aaaacgatgg gccatttgac cgaaggaact gaagtcatcg gggagcacgt ggcccatgcc
     5641 ctcgttggta cgatgttagg ggtgttctgt gcttacggtc ttatcgagcc aaccggcacg
     5701 aaaatggcgg cggacattga agcggaaggc cgttatttgg aagttatcaa agcggcgatg
     5761 atcgcctgtc aaagaggtgc accaccgatt gtctgtgtgg agtttggtcg tcgttctatt
     5821 atgccggaag agcgaccttc ctttgatgaa attgataagg cgacaaaaga aattaagaaa
     5881 gcggcgtaat catctatggc tgaaaagaag acgacgatcg tcattaagaa agtcacaatt
     5941 gttgcaggtg ggggtcatgg aggctcctgg aaggtggcac ttgcggattt catgacggcc
     6001 ttgatggcct tcttcttggt tatgtggtta ttaggtcaaa gtgatgaaac taaaaaagcg
     6061 gtttcagatt atttttcaac tccttcggtg attgaatata atttccaaaa ctttggcgtt
     6121 gaaatcactt tggaaaaact ctttctggat ttgatcaacg aacctttaaa ggcttttcaa
     6181 cagttcattg agcccgtggc caagactcca aatctcttgg atatgggttc gacgaaagta
     6241 gtgaccgcgt tcttggcgga tcaaatgaat gattacgcta aaaatgtcca agtcagtgct
     6301 gagggatttg attttgatat tcctgacacg gttctctttg aacgcgggac ctcaaatccg
     6361 ctgagtactt ttgtggatgt gatggataag atcaagggag tcacgacggg tctgcaagag
     6421 gctgaaatca aagtcacttc tggattgttc gtgcagtcgg ttccaggttc gaatgtggcg
     6481 ttagcaaata aagtgtccgc ccagcgtttg gatttggtga aaaacaaaat cctagccgga
     6541 cttgaattta acacggtgac cttgcagagc tacagcagtg tgaaggaaaa gcgcggggaa
     6601 gtggatccgc agaaactgat cggctttatt cgcctgagta ttcgtcagaa agatgtaacg
     6661 agcacaggca agaaaccgcg caagcttgaa acggtcttcg gtgatccgaa tgtaagcatg
     6721 tcagtctatg ataactttgt aaagcaggca gcagagcgca aatctcatga agaacccgca
     6781 ccaaagaagt tcggcaaggt gaaaaagatt gagaatccgg tgaacgagga aatgaaggac
     6841 cacaacgaac tcaacgatcg caacgatgaa gtgactccat tgcccgaagc gccgactgaa
     6901 gaatagattc gatccatcaa agcacgagca agcgcctcga cccgaggtgc ttgccatgat
     6961 taagtttaaa ttgaaggtta gtagggtgtt gaaaaaggcc tgattgacct ccaatttctg
     7021 aaatggttta aatgtcgtca tgcgtggata taaagaaaat cagcaagaag acatgttcag
     7081 ttacgtttca atggaggatg caatccccaa gaaacatccc cttcgtaaaa tcatggcgat
     7141 ggtggattcc ctgctggacg agatgtggtc agattttgac ggcctctatt cgggagttgg
     7201 tcgccccagc attccgccag agcaaatact aagaaactat ctcaaaacca tcataccgct
     7261 ccaattttat aaagtcgtct gcaccacaga agggcgcagg cgagctgaat aagaccaaga
     7321 taataaaggg cgtttttgtt gtagcgaata agaatgcctc gacactttga caaccacgct
     7381 aaggtcctct ccacgaccca ctttcgtggt gtcttcttgg ctgatacttt tccttggctt
     7441 tctgattttg aatttttttt aagccaagct tcatattctt gagggtccgc agggacccac
     7501 tcgcctcggt tgatctgagc tgtgtggctt tgatagccgt gttcttgaac cacttgctgc
     7561 gtattttgag tatcatagcc tttatccagg cacagatgct gagggttttt ttgttgacga
     7621 aggactctcc atcacgaccg cttccaaagt ctcctttaaa agtttcgagt catgacgatt
     7681 tgcttctgcc acatcaccgc caacggacct ccctcttgat cgacgagaaa actcttttta
     7741 gttccatttt tccctctgtc agtggggttt ttaccaactt tatctccacc catgcgggcc
     7801 ttttcaagac atccatcagc ggcttgccat tcccaataaa cttcacccaa gtcatcacac
     7861 tcttttgcaa tttcgctcca gatcttgatg aaaactccag atcgacacca cttcgtaaac
     7921 catctatgga cggtgcgatc agagccgaat tgctctggta aatggttcca ctgacagccg
     7981 gtacgaaatt gataaataat gcggtttata atttgcctcc agtttgctcg aggccgaccc
     8041 gtaggggctt caggaaagag attcattaaa ataggttcga ttcgctccca cagagaatcc
     8101 ggtatttccc agagggtttc caatggttct gatttgcttt ttactttttc tggattttga
     8161 agtttgcccc ttgtcttttt catcggaagt ctcctttcct ccttttggaa taagtggcat
     8221 tatatcactt attttgccga ctttcgcgtt gcataccttt tgctaataca gaccattgtc
     8281 acgccttgta tcgatctatt agctttagag ttcatctcta ggcggaaaaa cgtcagggag
     8341 tgacaaatat agtaactaaa tcgccctcac gggctaagga gcattttatg aaaaaagtag
     8401 ttttgtcggc actcattatg ctggcttctt ccgcggcatt tgctgatagc agttctggaa
     8461 ctgtttcggc tctccacgtc cacgaacact tcagcatgtt cgatattaaa ggtgagaccg
     8521 cacagaagat ttataacggt ctgcaaactc aagaaaaaga agatgggaat gccgcgggcc
     8581 aaaaaatggg cagcctttca aaagtcagcg ccgacggcag aatgtggtgc caaaaagatc
     8641 aaggcatcta caaatgccaa gtcacttttg atacaactac tggttcgatt aaccagactt
     8701 ggttgaagta acactgctta gcattttaaa aggccgtcag attttggcgg cctttttcat
     8761 acaaagccga tcaggctttt ttgatatatc ctatggacac cgactccaaa attgaaagat
     8821 ctcacctcac tcgtcaacgg atgactttga tttggtgacg caagaatagt ttctccaaac
     8881 tcaaaaagaa atttggaatt tggcttttag aaaatataca gcagcactgg aaaaaggaat
     8941 cgcaaaagaa tcggttaggg cacttctgcc tgcaggcaac ggatctggct gctccatttc
     9001 aatatgatgt ccggtcttga gattgatgga gctaaagatg acttttttgg cattatgtcc
     9061 agacagccaa aatcgattgg gctttccgcg acgagcccag gcagatctga gtggcgggaa
     9121 cagtaacaga tacgtctcat cctcgaataa aacgacattt ttccctctgt ggcgacgata
     9181 ccaacacttg agttcgttca ttttttttcg cttttgagga tcagtcagaa gttggtacct
     9241 gggtcttttc caaacatact tttgctcatg gagccgacgc cgaagcgatg tgctggagat
     9301 tttaattcca gccttcgcct gcaaatgaaa agataaaaga ccaaccgtcc aaacaactgc
     9361 ttgataaccc aattgatcgg gctgaatgaa ggatcaacgc tcggagcgga caaaggctac
     9421 gacactgctg atcatggaca ggatctgagt cagctaaata tcaaatccca tgttgcccag
     9481 aatatccatg ctcgtaaaaa gacaagttcc atcgacggac gaacgacaag ccactctggc
     9541 tatgaggtaa gtcagcggaa acggatgtgt atcgaagaga tctttggcta ggccaaaaca
     9601 attggcctgt tacggagacc gatgctgagg ggaacagaaa agctgggctg gatgttcagt
     9661 tttgtgatga gtgtgtacaa tcttgtgcga attaagaatt tattggcggc atagtgagga
     9721 taatgtcgga aaaacttccg ttggcgacga agttgatctc aactgaaact aattgaagat
     9781 aagtctggtt cggtgagaac gaaaaaaatc caaaaaagta aattttaaat aaaaaataaa
     9841 acggaaattt gatcaaacat aggtattttt caacaccctg ttagggattt gtggtttgtg
     9901 aaatcctgct agtccattga tgatgtccac tgatcctcga agcgctgcga cgtattttca
     9961 tatttaatta tttttccctt gtggggtgag ttgacgttct ttagtgagag acacactgga
    10021 tttacatatt cacttttatg agcctctgaa gcgccatata tcgctcacct tgcaataaaa
    10081 ttagccctaa gtctccggcc tcgtttgcga tgaaaactcc tcttgagccc gatttaaaca
    10141 ccaaaaattt gatcaaaaaa cttgtgttat aaagagggcg tcgatttatc cgacgctggg
    10201 gaacttaaca aaaaaaatta ggggttatta tggattcaaa tcattcagac aatctgaata
    10261 ctttcgaaga gaagcttaaa accctcgaag atcaagcttt gatcgagcag ctccagaaac
    10321 tctttgagag aaaaaaaaga attggcgatg cgatcctgct aaaccttcag gagatagctg
    10381 atcgtcgtct ttatgcgaag atgggttact ctagcatttt tgaatttctc gtaaaacact
    10441 ttcatctgag cgaatcggcg gcctatcaac gaacgaacgc cttaaaacta atccgcgaag
    10501 ttcctaaagc acaagaagcc cttttcaaag gcgaaacgaa tttatcaacg atggctgcaa
    10561 cccaaagttt tataaagcag cttgagaatg aacaagaggc tccactaacc aaggctgaga
    10621 aggaaactat ttttgatgcc gtaaaaggaa agtcgaaaaa agaagcccaa gctacttttg
    10681 ccgagatgaa tcctgtggct accttgccgg aaaccaaggc gaaggttctc accgctaaac
    10741 acaccttgtt gcaagtgacc ctcgaacaag agactctgga tcttattaac gaaatcaaaa
    10801 accttttaag tcatgaaatc cctgatggac atttaaataa agtccttaag cgaatggccg
    10861 aaattagtgt tggccaactt aaaaagaaaa aaggccgaag tatttcgaat gaaaattcga
    10921 attcaaaaaa ggaaagaact aaaaacacag tcgaaaaaga aaaagcgaat cctagcccta
    10981 ttttatcaga agaaacggag tgttctctct caagaagtca atctccaagt gatagcaact
    11041 gcccaaacga aaaccaccga tcgtcgaagc agcgcgcaag taaaagtcgc cacattccaa
    11101 tagaaattcg aagtcaggtt tttcagcgcg cccacggagg ctgtgaatat gtaagtcctg
    11161 atgagcaacg atgccaaaac cattaccaac ttgaggttga ccatatcacg agctggagcc
    11221 agggcggaga gacaagtctt gaaaactgcc gagttcactg taaaagccat aatcagtatc
    11281 gagtgaagga aacacacgga ttttggtatg aacattagag ttaaacagtc ctataaacag
    11341 gctcaattgc aggacttgga agtggttaat atctattttg ctggcaggcc attcattcca
    11401 ccaaacggcc ttggcactgg ctcttcattg gatagcggga cctaaaaact cgttgaagcc
    11461 ccaatcaaaa tcgccacatc cccttggccg ttggaaaatt gggtgggtgc cgaggtggtg
    11521 gtcccggtga tttgataaag aagattgttg ccacccttgt aaataaaact ggtgcgtaag
    11581 tcgaaatagt ttgtcaggtt tgcggcgctg accatggtat agccaatttc attgtcccat
    11641 ttccatgagg ggctgagctc tgtagtttta ccgaaagaga agtaggtgga ggattcatca
    11701 gtcccctccc aattcgtttc gctctcgtag cgaacgcggt actcctgcga agtgatggcc
    11761 aggtaagtgg tgttcccatc acgtgtactg gttttgaaat agtggttgtc aaagactccg
    11821 agcttgaaat ccgtgatcgt actgacttgg actctgagct ctgcattgat ttttaaaaga
    11881 aaatgctcgg tggaatcata gttaacatta gagccccaga ggcccaattt gaattgagga
    11941 ccgaagttga acccaaacgc tccttgtaaa gagggatctt tatttgtttg agtaagaccg
    12001 tgttttacat agttgctcaa aagagagatt tcgccattca ttttaaaggt gggaacttcg
    12061 cttttagcct cttgttgagc aaggcacggc atacacaaaa gaaggctggt aaacagcagt
    12121 gataatttta aagtaagttt gtagaaacat ctgtatttca taattttttt gtctttctat
    12181 taatttgact caaatcaaat tcaatgacaa acacaaacgc atatatggaa actcatagac
    12241 cttggaccga atatacattt gtcgcatttg atactgaaac cagtggggcc tatcccatcg
    12301 gatccgacat tattgaattt ggtgccgtca agtggcgggg aggccaagag atcgcaagct
    12361 tccagaccct actgaaacct cgagaattat taaccgagtt caatatgggc atccatggaa
    12421 ttagcaatga aatggtggcg aatgccccct tgatatctga gaagatccgt gaaatttatg
    12481 aatttatgga agattcagtg accatggctc atcatgcccc ttttgatatg gggtttgtca
    12541 ctgtggaact tgagactcat cgcttgccgc tgttgatgag tccagcttta tgcaccagtt
    12601 tactttcgag aaaatggatc catgggactg aaaaccacaa actgcaaacc ttggttaaat
    12661 ttttaaacat tgagggagga gcggctcatc gtgctctgga tgatgcccgc gcctgtttac
    12721 aagtgggtct tgagtgcttc aagcgtatgc ctgcagacgt gactttgacg gaggccatga
    12781 agagccaggg taaaaaattg caatggccag attattcctt actgcgttgg aataatcctc
    12841 tggtgaaggt cctgattgaa gctatttatc agaaaaaaaa tgctgagata ctttatgcga
    12901 actcctctgc atcgagttcg gagcctcggc aggtgacgcc gatggggatt gtacgcaacc
    12961 ctgacggaga ttatctccag gcgttttgtt tgcgtgataa aactagtaaa aggtattatt
    13021 tagcgaaaat taaggacgcg gcccttgttt attag
//
LOCUS       DATBTE010000240         4405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_304909, whole genome shotgun sequence.
ACCESSION   DATBTE010000240 DATBTE010000000
VERSION     DATBTE010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304909"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1037)
                     /locus_tag="VIG33_08885"
     CDS             complement(36..1037)
                     /locus_tag="VIG33_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8270991.1"
                     /translation="MKLFISAAITFILFMGFQVRAQTTIKVDGSSTVFPITEAMAEEF
                     QAAQKNKIRVTVGISGTGGGFKKFCRGETDVQDASRPIQSSELEACRKAGIRFVELPV
                     AYDATVVVVNPKNEWLKEAKIEDLKKMWEPEAQGKVTKWKQVNPAWPDENLKLFGAGS
                     DSGTFDYFTEAIVGKAKSSRGDYTASEDDNTLVTGVANDKYALGYVPLAYYVENKAKL
                     KAVAIIGGDKSPAKNKAVLPSKETVENGTYFPLSRPIFIYVNEASLKKTEVQDFVKFY
                     LQNAEKLVPQVKYVALPKKAYEIGLEHLSKKKLGTVFAGHSDVGIRIEELMKKESKE"
     gene            1172..2338
                     /locus_tag="VIG33_08890"
     CDS             1172..2338
                     /locus_tag="VIG33_08890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8270992.1"
                     /translation="MIYTPGCLLNPQKEFEQLNFCLKTAHIGIWEASLSKTSLEFYSL
                     KWDEQIRDMHGIPQAQEVAPLEWYCQNIHPDDKLFLRENVSLFLEKKHPSQTMTSVYR
                     TRWPNGEIHSIEIYASFEDLGPNEDNLFLRGIAKDITQDIQKQKLLEDQKNYILTASR
                     MALLADISAGIAHEINNPLTVIQARSFQLTQMVDHGNLDPVKIKSAAESISKTGDKIA
                     KIIQSLRSFAREKETEPLDTLNVAQLIQETLDFCKVRFYKHGIDIRLGDIPENLEFEC
                     HLLQIEEALLNIFNNAHDAISKLQEKWILIEAFDKETNIEIHVTDSGNGIADDIADKI
                     MLPFFTTKKIDKGIGLGLSITAEIINKHHGTFFLDRTADHTKFLIRIPKVQPAE"
     gene            2454..3155
                     /locus_tag="VIG33_08895"
     CDS             2454..3155
                     /locus_tag="VIG33_08895"
                     /inference="COORDINATES: protein motif:HMM:NF025631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4360 domain-containing protein"
                     /protein_id="HEY8270993.1"
                     /translation="MKDAWKEFLFAMNAAFIFLLILMVTEMTLGQTPEVPERARILSA
                     QTVGTGCEATQTSVSLSPDLLELSVLFSNYSLELGAGTASPMAPVQQKNCQINVELEV
                     PAGWALAIQSVDYRGYVHLPLGATARHRFSYHTDGMQPAMLREAPMRGPLDQNYFFRL
                     EQPLQERPFTPCGPPNIHLRLGSRMALHYPPGPQRRTGEVRETALLSLDSADLSLKQD
                     FQIEWKRCGRKNAVK"
     gene            complement(3194..3736)
                     /locus_tag="VIG33_08900"
     CDS             complement(3194..3736)
                     /locus_tag="VIG33_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270994.1"
                     /translation="MHFRKVFSSLALLSLLGCASTPSMKSEKTGEVNPEVADSSLASE
                     TLAINPEPTKKSDSGYLICKASKRRFHYDWSAGSLRNGQEVIDQNNIRYEFRSAGKPA
                     GNKGENLKVGECGWAKVALVSRKKAPKSQVKSQVIFKSLSDEATQTFYKMKTGKVFKF
                     PVRQTKNGLEAMPGGIRLIR"
     gene            complement(3754..>4405)
                     /locus_tag="VIG33_08905"
     CDS             complement(3754..>4405)
                     /locus_tag="VIG33_08905"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEY8270995.1"
                     /translation="MIIGLVLFFTLGTWSIAQVSGGLEQSWFMQYILQKWSLMNHETA
                     ASISYFIFFLFAILLIIPLSYLLSVLLVSQLLLPSILKIIEKKDFPELEKKKGGSLSY
                     GFWNTLKASGVYLISLVVTLPLWLFPGFAVVWPVLLSSYLNKTVFVYDVLEDFASEEE
                     RKYLEKEHGRSLYVLGIILGFINYIPLTFIVVPTFSGLAYSYFCLNALKDLRKNKL"
BASE COUNT         1279 a         1042 c          849 g         1235 t
ORIGIN      
        1 aaattccgca agttttcgag aacttctttt agtcattact ctttagattc ttttttcatc
       61 aattcttcaa tgcgaatgcc cacatcggaa tgtccggcaa agacggtgcc caattttttc
      121 ttacttaaat gctcaaggcc aatttcatag gcttttttcg gaagagctac atatttaact
      181 tgaggaacaa gcttttcagc attttgcaga taaaacttca cgaagtcttg aacttctgtt
      241 tttttcaaag aagcttcatt gacataaatg aagatcgggc gagaaagggg aaagtaagtt
      301 ccattctcca cggtttcctt tgagggaaga acagctttgt ttttagctgg agatttgtca
      361 ccaccaataa tcgccacggc ttttaatttt gctttatttt ccacatagta tgccaaagga
      421 acataaccta gagcatactt atcattggca acgcctgtga ctaaagtgtt gtcatcttca
      481 cttgccgtgt aatcaccacg gctggattta gcctttccca cgatagcttc tgtaaagtag
      541 tcaaaggttc cagagtcaga accagctcca aaaagcttta agttctcatc tggccaagcg
      601 ggattgactt gcttccattt agtcactttc ccttgagcct caggttccca catctttttg
      661 agatcttcga ttttggcttc tttcaaccac tcatttttag ggtttaccac gaccactgtg
      721 gcatcataag caaccggaag ctccacaaac cgaattcccg ctttcctgca ggcttcaagt
      781 tcagaacttt gaatggggcg agaagcatct tgcacatccg tttccccgcg acagaatttt
      841 ttaaagccac cgccagttcc tgaaatccca actgtcacgc gaattttatt tttctgagcc
      901 gcttggaact cttcagccat cgcttcagtg atgggaaaaa cggtgctaga accatccact
      961 ttaatagtgg tttgagccct cacttgaaaa cccataaaca aaatgaatgt aatagctgca
     1021 gaaatgaata acttcatagg tgctccttca atttgtccag acactaacat cggcttcctt
     1081 cttggtcttg tcagggtttt attaggattt cagcttagcg cgttaaggtt gccgactttg
     1141 acttatgttc caatagtcat aagcttactc aatgatttat accccaggct gtcttctcaa
     1201 tccgcaaaaa gaatttgaac aacttaattt ctgcctcaag acagcccata taggtatctg
     1261 ggaagcctct ctcagtaaaa cctccttaga gttttacagt ttaaagtggg atgagcaaat
     1321 tcgtgatatg catggaatcc ctcaagccca agaagtcgct cccttggagt ggtactgtca
     1381 aaatatacat cccgatgaca agctttttct tcgtgagaat gtcagtcttt ttcttgaaaa
     1441 aaagcatcct tcacaaacca tgacctctgt ttatcgaacg cgttggccga acggagaaat
     1501 tcattccatt gaaatatacg ctagttttga agaccttggc cccaatgaag acaacttatt
     1561 cctccgcggc atagccaaag acatcaccca agatattcag aaacaaaaac tcttggaaga
     1621 tcaaaaaaac tatattttaa ccgcctcacg tatggcgctc ttggccgaca tctcagcggg
     1681 cattgctcac gaaattaaca atcccctaac ggtgattcaa gccagatctt ttcaactcac
     1741 gcaaatggtc gatcacggaa atttagatcc tgtaaaaata aaatcggcgg cggaaagtat
     1801 cagtaaaacc ggtgataaaa ttgccaaaat tatacagtcc ctgaggagct ttgcccgtga
     1861 aaaagaaacc gaacctcttg atactctcaa cgtcgctcaa ctcattcagg aaactctcga
     1921 tttttgtaaa gtgcggttct ataaacatgg catcgatatt cgccttggcg atattcccga
     1981 aaatttagag tttgaatgtc accttctgca aatcgaagaa gctctgctaa atatcttcaa
     2041 caatgcccat gatgcaattt ctaaattgca agaaaaatgg atcctcattg aggctttcga
     2101 taaagaaacc aatatcgaaa ttcacgtcac tgactcagga aatggcatcg ccgatgatat
     2161 tgctgacaag attatgttgc cttttttcac caccaagaaa atcgacaaag gcatcggctt
     2221 aggactttcc atcaccgctg aaattatcaa taaacatcat gggacttttt tcttggatcg
     2281 cacagcggac catacgaagt ttttaatacg catccccaag gtccagcccg cggaataaaa
     2341 aatctgattc aaaaaatgag caagggtcat tttggctcta aagaagtagg cccttggttt
     2401 gcaagtgaag atctcgagtc tgacgtaaag tcatttaatg aggggatcca acaatgaaag
     2461 acgcctggaa ggaatttcta tttgcaatga atgccgcttt catattttta ttgattctga
     2521 tggttactga aatgacctta gggcaaaccc cggaggtgcc agaacgcgct cgcattcttt
     2581 cagcgcaaac agtcggcact ggctgcgaag ccacccaaac ctctgtcagc ctctctccgg
     2641 atctgctgga gttatcggtt cttttttcga actacagcct ggagctagga gctggaaccg
     2701 cgtctccgat ggctccggtt cagcaaaaaa attgtcaaat caatgtcgag cttgaggttc
     2761 ctgcgggctg ggccttagct attcagtctg tagactatcg aggctacgtt catctccctc
     2821 tgggggcgac cgcgcgacat cgattttctt atcataccga tgggatgcaa ccggcaatgc
     2881 tgcgggaggc ccccatgcgc ggccccttag accaaaatta tttttttcgc cttgaacaac
     2941 ctcttcagga aagacccttt actccctgtg gtccgcccaa tattcattta cgtttaggct
     3001 ctcgcatggc tttgcattat cccccaggtc cccagcgccg taccggagag gttcgtgaaa
     3061 ccgccttact aagtcttgat agtgcggatt tgagtctcaa acaagatttt caaattgaat
     3121 ggaaacgctg tggacgaaag aatgctgtga agtaataata gtttcatgaa taaaacggac
     3181 ttatctttaa gccttagcga atcaggcgaa tgccacccgg cattgcctcc agcccattct
     3241 ttgtttgccg tactggaaat ttaaaaactt ttccggtttt cattttataa aatgtttgtg
     3301 tcgcctcatc ggagaggctt ttaaaaatca cctgtgactt tacttgtgac tttggagctt
     3361 ttttcctgct gaccagagcc actttagccc aaccacattc ccctactttt agattctcac
     3421 ctttatttcc cgcaggtttt ccagcggacc ggaattcgta acgaatgttg ttttgatcaa
     3481 tcacttcctg accatttctc agggagcccg cgctccaatc ataatgaaaa cgccttttgc
     3541 tggccttaca tattaggtaa ccgctgtcag actttttcgt aggctctgga ttgatagcta
     3601 aagtttcaga cgccaatgag gagtccgcca cctcgggatt tacttcgcct gtcttttcac
     3661 tcttcatgga tggagtgctt gcacatccta ataaagacaa caacgccaaa ctagaaaaaa
     3721 cctttcgaaa atgcatattc actcctcaat caatcagagt ttatttttcc gcaaatcctt
     3781 cagggcattc aagcaaaaat aactataagc tagaccggag aaggtgggaa ccacgatgaa
     3841 ggtcaaggga atataattaa tgaagcctaa gataattcct aagacataca gcgatcgtcc
     3901 atgttctttt tcaaggtatt tacgctcttc ctcactcgca aaatcttcaa gtacatcata
     3961 aacaaatact gtcttattaa gataagaact caataaaacc ggccagacga ccgcgaatcc
     4021 ggggaaaagc caaagcggca aagtcacaac cagtgaaatc aaataaactc cactcgcctt
     4081 caatgtattc caaaaaccat aagacagact tccacccttt ttcttttcaa gttccggaaa
     4141 atctttcttt tcaatgattt ttaatatgga gggtaacaac aactgggaca ccaaaagcac
     4201 ggagagcaga taagacagag gaataattaa taagatagcg aacaaaaaaa agataaaata
     4261 cgaaatcgaa gccgccgttt cgtgattcat cagcgaccac ttctgcaaaa tatattgcat
     4321 gaaccaggat tgctctaggc ccccgctgac ttgagcaata ctccaagttc caagagtaaa
     4381 aaaaagaacc agtccgataa tcatg
//
LOCUS       DATBTE010000241         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2012449, whole genome shotgun sequence.
ACCESSION   DATBTE010000241 DATBTE010000000
VERSION     DATBTE010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2012449"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            456..1640
                     /locus_tag="VIG33_08910"
     CDS             456..1640
                     /locus_tag="VIG33_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8270996.1"
                     /translation="MSLAHERARQVMSLLLKKIKFSPIVSIQGPRQCGKSYLAAKLLP
                     KKLSSTDFKTLDTRETRQLADQNPRIFLESTNKKHLIIDEVQKVPHLFDEMKAVVDEN
                     RKPGQFIVLGSTEFSIEAKIKESLTGRLSRIKVYPLNLSETKKMELNPIRNFPFVGNK
                     ARVHRKEVLKYLVNGGLPGIFVVKSELEKHNLMRDWIDLTVTRDIFQFKDKNLDSELA
                     EQILQCISTLDEATAIFISNATGKSTSVIQKHIKALKNLFVIDEVKPYLKSAGKPVYY
                     ICDIGILNYYKATLDKKVTTWLLLELKSQLSYKGEQSDKIFYFKSARSKPIQFIHQIG
                     KKLTAVKVVSADSFDQRDLLIFESIKKKHPHLEHCFHVLYGGSQQLEIDNILISPWEY
                     VV"
     gene            complement(1647..2150)
                     /locus_tag="VIG33_08915"
     CDS             complement(1647..2150)
                     /locus_tag="VIG33_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY8270997.1"
                     /translation="MQTTTRNTNATQTRSRQRKIRNAEKVPLPRTSTHLNELLEWSAH
                     PNPKSIKFVHRHYARDVFFKDPFRELYTTEDLQKYYQKTLERLNDVHFSFENVLEHGQ
                     QAFVTWVMTAHFMKREFSVHGTSHFKFNSSGLCEYHRDYFDLSEEIYEHLPVVGYVFK
                     GLRRVLN"
     gene            2261..>2596
                     /locus_tag="VIG33_08920"
     CDS             2261..>2596
                     /locus_tag="VIG33_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEY8270998.1"
                     /translation="MIKDKKILSWALYDWANSAYATTVMAGFFPLFFQKYWSAGADST
                     ITTTRLGTAISISSLIIAMVSPVLGAVADLRSHKKLYTFLFMLVGVLSCAWMFFIPRG
                     DWWSAILAYG"
BASE COUNT          784 a          457 c          550 g          805 t
ORIGIN      
        1 gagtcatctg ccgttgaagt tggtgcttca aaatttgaat tttttgcgca acaaatgttg
       61 gtcgtcatga tgagagcgca gagtgtgaat gaaaaaatat ttgttttcat gggtccccct
      121 gtggagaact catgcagcaa atagagtgct gatatgacga agagccagta aaaggaacaa
      181 cggattggaa gaggtttgta agtgattaaa attgtttcat aaaaaaaatc agcaaaagtt
      241 catttgtttc ttcttttggt gacaatattt tgactctgtc agagtcctga ctgtttggtt
      301 tttggggttg ttttcgcccg gtgagtggaa ttctcgattt ctaaataact ttgacagtct
      361 gagtatgcct gtttatcata aaaatagacc ccgtcaatta ttaaaaatat ttcttcattt
      421 ttgacagggt ctgatttttt taaaatggag tacatatgtc tttagcacat gaacgggcgc
      481 gccaagtaat gagtcttctt ttaaaaaaaa ttaaattctc gccgatcgtt tccatccagg
      541 gtccgcggca atgtggaaaa agttatttgg ccgccaaact actaccaaaa aaattgagct
      601 ctactgattt taaaacactg gataccagag agactcgcca attggcggat caaaacccca
      661 gaatattttt agaatccaca aacaagaagc atttaattat tgatgaggtt cagaaggttc
      721 cacatttatt tgatgaaatg aaggctgttg ttgatgaaaa tcgaaagcct ggtcagttta
      781 ttgttcttgg ctcaacggaa ttttcaatag aagcaaaaat taaagagtct ctcaccggaa
      841 gattatcaag aattaaagtt tatccactta atctttcaga gacaaaaaaa atggaactta
      901 acccaataag gaattttccc tttgttggaa ataaggcaag agttcataga aaagaagttc
      961 taaaatattt agtaaatgga ggacttcctg gcatatttgt cgttaagtct gaacttgaaa
     1021 agcataatct aatgagagat tggattgacc tcacagtgac tcgggacatt tttcagttta
     1081 aagataaaaa tttagattcc gaattggccg agcaaatcct ccagtgcata tcaactcttg
     1141 atgaagcaac cgcaattttc atttcaaatg cgacaggaaa atcaacatct gtcatacaaa
     1201 aacatataaa ggcactgaaa aatttatttg taattgatga ggtaaagccg tatctgaaat
     1261 cggcaggaaa acccgtctat tacatatgcg acattggaat tttaaattac tataaagcca
     1321 ccttagataa aaaagtaaca acttggttgc tgttagagct gaagtcacag ttaagctaca
     1381 aaggagagca atcagataaa attttctatt ttaaatccgc acgcagtaag cccattcaat
     1441 tcattcatca gatcggaaag aaattgactg ctgttaaggt tgtctccgct gactcgtttg
     1501 atcagaggga tttattgatt tttgagtcga ttaaaaagaa gcatcctcat ctagaacact
     1561 gttttcatgt tctttatggt ggaagccagc agttagaaat agacaacata ctcataagtc
     1621 catgggaata tgttgtctag actgatttaa ttaagaactc gtcggagtcc tttaaataca
     1681 tagcctacca cgggcaaatg ctcataaatt tcctcggaca aatcaaaata atcgcgatga
     1741 tattcgcaaa gaccactgct attaaatttg aaatgcgaag tgccgtgcac tgaaaattcg
     1801 cgtttcataa aatgggctgt catcacccac gtcacaaagg cctgttggcc gtgctctaaa
     1861 acgttttcga aagaaaaatg cacgtcattg agtctttcta atgttttttg ataatatttt
     1921 tggagatctt ctgtggtgta aagctctcgg aagggatctt taaaaaaaac atcccgggca
     1981 taatgtcgat gcacgaattt tatgcttttt gggttggggt gcgctgacca ctctaagagc
     2041 tcattgaggt gggtcgaagt tctaggtaag gggacttttt cagcatttct aattttccgt
     2101 tgtcgagaac gtgtctgagt tgcgttggta tttcgcgttg ttgtttgcat atatctcctc
     2161 catggcagca tttttcagga aaaaaggggg atcggtcgaa tcatgttttc gtcacaaagt
     2221 gaggttgatt cattaaatat tccatggttt gatctttgac atgatcaaag ataaaaaaat
     2281 tctcagttgg gcgctttatg attgggctaa tagtgcctat gcaacaacgg tcatggcggg
     2341 attctttccg cttttttttc aaaaatactg gagtgccggg gcggactcca cgattaccac
     2401 cacgcgctta ggaaccgcca tctctatttc cagcttgatc atcgccatgg tgagtccggt
     2461 tttaggagcg gtggcggatt tgcggtccca taaaaaactt tatacctttc tttttatgct
     2521 tgtgggggtt ttaagctgcg cctggatgtt ttttatcccc cgaggtgatt ggtggtcggc
     2581 gattttggct tatggt
//
LOCUS       DATBTE010000242         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_167584, whole genome shotgun sequence.
ACCESSION   DATBTE010000242 DATBTE010000000
VERSION     DATBTE010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167584"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VIG33_08925"
     CDS             <1..719
                     /locus_tag="VIG33_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8270999.1"
                     /translation="LTACLLIYVGTIEGYAYWNQKRIGHVGISDIAQHQIFANYMARG
                     ILKEEGAKEDASAELKAFTRAYLEMDPKLRDSQYSLFEKWAELNPGKDLYTELVPVNK
                     ELLSKRPFGYVAAALRNLSSLIDGRASFNYYPIVDRDIPSKLYFWLQFALDNIFCVLW
                     FTALLYFFATIRRRGLFCVENLIFLTVATQFMTIAVLGYGELRRQSVGVAGVQMVFVL
                     VMWMHLLKRRWQKKAGLTCA"
     gene            662..997
                     /locus_tag="VIG33_08930"
     CDS             662..997
                     /locus_tag="VIG33_08930"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY8271000.1"
                     /translation="MDAFAKTALAEEGWIDMRLSLDEKQAILDTILKFDPEAEIYLFG
                     SRTDSSKKGGDIDLLILSKKLSEDYKRLIRNDICDIIGEQKIDVLIAENESSPFVRKA
                     KRNGIKLND"
     gene            975..1430
                     /locus_tag="VIG33_08935"
     CDS             975..1430
                     /locus_tag="VIG33_08935"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEY8271001.1"
                     /translation="MESSSMTEDQEILKESLEELRQAKQWLHHSYSLCKALQINMNME
                     IESFDKLEALTSRFARLVDLLVNKTFRTIDTLELEYGGSLIDVVNRAAKRGIVDSVEQ
                     VREFKDLRNEIAHEYAKRDLTRIFKNVVQLTPSVLDLCEKTEQYCLKWK"
     gene            1594..>1838
                     /locus_tag="VIG33_08940"
     CDS             1594..>1838
                     /locus_tag="VIG33_08940"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8271002.1"
                     /translation="MDRYLKKALLKDLFKKIIIITGPRQVGKTTLSKSLLRELTKDFD
                     YYNYDNPQDRKKIHRGDLDFNYRPLVLDELHKMRQWK"
BASE COUNT          565 a          349 c          390 g          534 t
ORIGIN      
        1 ccctcaccgc ttgtttgctg atttatgtgg gaactattga aggctatgcc tattggaatc
       61 agaagcgcat tggtcatgtc ggtatttcag acattgctca acatcagatc ttcgccaatt
      121 acatggcgcg cggtatttta aaagaagaag gagcgaaaga ggacgcttca gcggaactca
      181 aggcctttac ccgcgcttat ctggaaatgg atcccaaact cagggactct caatactctt
      241 tatttgaaaa atgggcggag ctcaatcctg gaaaagatct ctataccgaa ctcgttccgg
      301 tcaataagga gctcctgtca aagaggcctt tcggatatgt cgcggcggct ttacgcaatc
      361 tttcttcgtt gattgatggc agagcgagct ttaattacta tccgatcgtg gatcgagata
      421 tcccctcgaa gctttatttt tggctgcaat ttgcattgga caatattttc tgcgtgcttt
      481 ggttcacggc tttgctttac tttttcgcta caattcgaag gcggggcctt ttctgcgtgg
      541 agaatctgat tttcctcact gtggcgactc agtttatgac aatcgcggtg ttgggatatg
      601 gggaactccg acgtcaatcc gtaggtgtcg ctggggtgca gatggtgttt gtgttagtaa
      661 tgtggatgca tttgctaaaa cggcgttggc agaagaaggc tggattgaca tgcgcttaag
      721 tttggatgaa aaacaagcaa ttctcgatac aattttaaag tttgaccccg aggctgaaat
      781 ttatcttttt ggatccagga cggactcctc caaaaaaggc ggagatattg acttgcttat
      841 actttcaaaa aaattgtctg aggattacaa acgacttatc agaaatgata tttgtgatat
      901 aattggggag caaaagattg acgtcttaat cgcagaaaat gaatcatcgc cgtttgtaag
      961 aaaagctaaa agaaatggaa tcaagctcaa tgactgaaga tcaggaaatt cttaaggaat
     1021 cattagagga acttagacaa gcaaagcagt ggcttcatca ttcatacagc ctctgtaagg
     1081 cccttcaaat caatatgaat atggaaatcg aaagctttga caagctagag gccttaacaa
     1141 gtcgctttgc tcgtctggtg gatcttcttg taaataagac ttttagaacc attgacacct
     1201 tagagctcga atatggaggt tccctcatag atgttgtcaa tcgcgccgct aaaagaggta
     1261 ttgtggactc cgtcgaacag gttcgagaat tcaaagatct tcgcaatgaa attgctcatg
     1321 aatatgcaaa gcgcgattta acccgaattt ttaaaaatgt agttcaactc acaccgagtg
     1381 ttttagatct ctgtgaaaaa acagaacaat attgcttaaa gtggaaataa aaaattagat
     1441 ttctttctca gtgataccat tttcatcgca taactcaagc tcataaacta gatatcaatt
     1501 caaatacaat tgatttcatg taaatttaag actttttcgg tattaaaacc gaattagact
     1561 tgactttttc ggtattaaaa ccgaaaaagt attatggata gatatctcaa aaaagcgctc
     1621 cttaaggacc ttttcaagaa aattattatt atcaccggac ctcgacaggt aggaaaaacc
     1681 accctctcaa agtcattact cagagagttg accaaagact ttgattatta caattacgac
     1741 aatccccagg accgaaaaaa aattcataga ggagatttag attttaatta tcgacctctt
     1801 gttttggacg aacttcataa aatgcggcaa tggaaaag
//
LOCUS       DATBTE010000243         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_919237, whole genome shotgun sequence.
ACCESSION   DATBTE010000243 DATBTE010000000
VERSION     DATBTE010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919237"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VIG33_08945"
     CDS             complement(<1..249)
                     /locus_tag="VIG33_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY8271003.1"
                     /translation="MENNNNSSSSSTVLPSSQNNQQVLWSTQVQKSSENKTIVYQSAI
                     MGSLMKMIDRVAPSNANILVLGESGTGKELVARYIHEKS"
     gene            398..1324
                     /locus_tag="VIG33_08950"
     CDS             398..1324
                     /locus_tag="VIG33_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY8271004.1"
                     /translation="MAIRPLNLSDSLTKYLKYMDFVKSSSPLTLKAYKKDLEQAFDLK
                     NSSSSLKKLKNLNEAELLVLTREALNGWACLSLSSRNRKSATLKSFFNWAYQEQLTEK
                     NLAIQITCPKVPKKIPHFLSVDEALAVLHSYDSKDVSLQNLRDKALFLVLYGAGLRVS
                     EACNLEWADIQFSSRSLRLVGKGGKERLVILPKLAIEILQRLQRISNDKFVFAAAEAL
                     DPRVAYEWVRSRGAKAQLMKPLHPHALRHSFATHLLSSGANLRTLQELLGHESLQATE
                     KYTHLGIDQLAQTLETYHPLSHLPSRKKAEGY"
     gene            complement(1329..1937)
                     /gene="recO"
                     /locus_tag="VIG33_08955"
     CDS             complement(1329..1937)
                     /gene="recO"
                     /locus_tag="VIG33_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY8271005.1"
                     /translation="MEVKERFIILRKIKYSEADLILHALSPRGEKLSFLARGALKSKK
                     RFGGGVLDPLHFVQFTYKQASNEGKMNTLSEATLLNDFKKVRQDYDHLELALHVLDCV
                     SRVSQEGDHSSEALFNLLGNTLKSLESCQDVSLLKIHFYLKFLMQQGVLNPEPWMGPL
                     LKTNIMESNSLAGDLALKKEISLHSASMEFITQQYLKTAEHH"
     gene            complement(1971..>2445)
                     /locus_tag="VIG33_08960"
     CDS             complement(1971..>2445)
                     /locus_tag="VIG33_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271006.1"
                     /translation="ICGERYKTPPLIAPQMSWNFSQSRELINKIYVSPQDQKLVESFE
                     QSFSEIAKPWFSQPTPRLFDRVLLKFPEVSGEALLQRLYDQLLVDPSKYYETMDTTNF
                     CRWHHFKTFSAWWEPKPAVEDLESLLVIDSGWLVTKDFAKILKTTYEEIKAEQSW"
BASE COUNT          700 a          543 c          500 g          702 t
ORIGIN      
        1 ggacttttcg tgaatgtaac gagcaaccaa ttccttaccg gtgccggact ctcccaagac
       61 cagaatattc gcgttagacg gagctactcg atcaatcatt ttcataagtg agcccataat
      121 tgcagactga tagacgatcg tcttattctc gctcgatttt tggacctggg tgctccaaag
      181 aacttgctgg ttgttttggg aagaaggtag aactgtagat gaggaagaag aattattatt
      241 attttccatt acgtgccgcc tttaaaagta tgtttttgat acaatgcgtt aacacgatgc
      301 aatataatta acgcctgtaa ttaatgcaag tgattttttg catggcatta tattttttcc
      361 caggctcact tataatctaa aggagaagga ttccaagatg gctattcgac ccttaaatct
      421 ctccgattca ctaactaagt atctgaaata tatggatttt gtaaaatctt catcccctct
      481 taccttaaaa gcttacaaaa aggatttaga acaggctttt gacctcaaga actcatccag
      541 ctctttaaaa aaattgaaaa acttgaacga agctgagcta ctggtcctga cccgggaggc
      601 tcttaatggc tgggcttgcc tcagcctttc ttcacgaaat cgaaagagtg ccaccctcaa
      661 gagcttcttc aattgggcct atcaagagca acttacagaa aaaaacctag cgatccagat
      721 tacctgtccc aaggtcccga aaaaaatccc tcatttcttg agtgtggatg aggccctggc
      781 ggttcttcat tcttatgact ctaaagatgt gtctctgcag aatttacgag ataaggctct
      841 ttttttagtg ttgtatggag cgggcttgcg agtcagtgaa gcctgcaatt tagagtgggc
      901 tgatattcaa ttctcttccc gcagtctgcg gcttgtgggc aagggaggta aagaacgtct
      961 ggttatttta ccgaagcttg cgatagaaat tttgcaaaga ctgcagagaa tttccaatga
     1021 taaattcgtc tttgcagctg ctgaagcttt ggatccccgt gtcgcctacg agtgggtacg
     1081 cagccgtggt gccaaggctc aactgatgaa gcccctccat ccccatgctc tcagacacag
     1141 ctttgccact cacctactca gtagcggagc caatctcaga actctgcaag agcttttagg
     1201 tcatgaaagt cttcaagcca cagaaaaata cactcatttg ggaatcgacc agttggctca
     1261 aactcttgag acctatcacc ccttgagtca tcttccttcc agaaaaaaag ctgagggata
     1321 ttgaaaactt aatgatgttc cgcagtcttc agatattgct gagttatgaa ttccatcgag
     1381 gcggagtgaa gacttatttc tttctttaaa gccagatctc ccgcaagaga attgctttcc
     1441 ataatattcg tctttaacaa aggccccatc caaggctctg ggttcaacac cccttgttgc
     1501 atcagaaatt tcaggtagaa atgtattttt aataaagaga catcttggca ggattcaagc
     1561 gactttaagg tgtttcctaa aagattaaaa agtgcttcgg aactatggtc cccttcttgg
     1621 cttactcgtg aaacacaatc taaaacatga agagctagtt caagatgatc ataatcttga
     1681 cggacttttt taaaatcatt cagaagagtg gcttcactca aggtattcat ctttccttca
     1741 ttcgaagctt gcttgtaggt aaactgcacg aaatgcaaag gatccaaaac ccccccacca
     1801 aaacgctttt tacttttaag agctccacgc gcaagaaaag aaagcttttc acccctggga
     1861 gacaaagcgt gcaaaatgag atctgcttca gaatatttta ttttgcgaag aataataaaa
     1921 cgctctttga cttccattta agatccacca ataaacaaca ctttgagaag ttaccagctt
     1981 tgttcagctt tgatctcttc ataggtcgtt tttaggatct tggcaaaatc ttttgtaacc
     2041 aaccacccag aatcaatcac cagtaaggat tccaaatcct cgactgcggg ttttggctcc
     2101 caccaggcac taaaagtctt aaagtgatgc catcggcaga aattggtcgt atccatggtt
     2161 tcgtaatact tcgacgggtc cacgagcagt tgatcataaa gtcgttgcag gagcgcttca
     2221 ccgctgacct ccgggaattt caaaaggacc cgatcaaaaa gtcgaggcgt cggctgacta
     2281 aaccaaggct ttgcaatttc tgaaaaagat tgttcgaaag attcgacgag cttttgatcc
     2341 tgtgggctga cataaatttt attgatgagt tctctgcttt gcgagaagtt ccaactcatt
     2401 tgcggcgcga ttaatggcgg agtcttatag cgctctccac aaatt
//
LOCUS       DATBTE010000244         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2218410, whole genome shotgun sequence.
ACCESSION   DATBTE010000244 DATBTE010000000
VERSION     DATBTE010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2218410"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VIG33_08965"
     CDS             <1..629
                     /locus_tag="VIG33_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271007.1"
                     /translation="TLTVRVFDKKFTIRDGHLEPLRKDLALYYTGNLELSPEHKQKIE
                     VAPYITAEFRVKNDKVHGTLLRGYVYQKLLEFKGESLKEQTRLLYPIKRIEQFFVDRR
                     TDPYYQDLISQLERTLALIQQGDREALNWSSMILNQAETALENVFTGDKLLTLLVRDL
                     RHTSESTRKLNPSAKMDPAINEADEECLKTAPTKTPTITPKPNYDLTN"
     gene            560..1519
                     /locus_tag="VIG33_08970"
     CDS             560..1519
                     /locus_tag="VIG33_08970"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY8271008.1"
                     /translation="MSENSSHKDSNNNSETKLRLNKLIADSGLASRRHADRMIEEGQV
                     TVNGKRVYELGIKVDPAHDHILVNGKPLKKPLENIYILFHKPKGVLTTMEDPLGRPTI
                     AEYLDGVPGRVFPVGRLDWDSEGMLLLTNDGEFANKVTHPKAEVTKTYMVKVDGQPTK
                     EEIQKLVRGVSIIGGKVAAKHIEKIKRQEGSDKYDWYKMVITEGKNRQIRQMFEKIGY
                     DVMKLQRVAIGRLRIGAVERGELVFLNDAAVERVFLPDAPDEIKLKKTYKGRAIAQRI
                     AAGKTADGKSSARKAKTGSKPGVKAGVKSRSKTGYKPKKSFNK"
     gene            1522..2394
                     /locus_tag="VIG33_08975"
     CDS             1522..2394
                     /locus_tag="VIG33_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8271009.1"
                     /translation="MNRKQLVGIFEITLGSIGFAFLGIFGKWAFSSGLSVGALLTYRF
                     IIASMALWMGLFLFNRDKIYISRTQLLISAGLGVFGYAVFATLYFSAVQGMSVGLAAL
                     LLYTYPFWVSLLGHFFLRERMNQTQWLLLLGAFLGVTCLLWGQFHLNNMKAFIAGLGS
                     AITYAIYILFSAHYQKNVKPLSSSLYVTTFAAIALASYHHPPLQFWKILDFQQWQIVL
                     GIATVSTIFPMTLILGGLQKLKSSQAALITMLEPVIAAIAAGILLHESMSGLQIVGAT
                     LVMTCLGLSVLRAH"
     gene            complement(2391..>2832)
                     /locus_tag="VIG33_08980"
     CDS             complement(2391..>2832)
                     /locus_tag="VIG33_08980"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HEY8271010.1"
                     /translation="YIQETYLCFEVESYSPKIRERLTKSRKTYAIDTGLFNALSENPQ
                     RTLGRQLENLVFLELKRRKKEISFFKNKNFEVDFICTKNRKIQDLIQVSWDISDMKTR
                     TREFNGLFQGKNELGYGQPLLLTYDYEDVIDGVVVMPVWKWLLG"
BASE COUNT          783 a          661 c          618 g          770 t
ORIGIN      
        1 aaaccctcac agttcgggtt tttgataaaa aattcacgat tcgagatggg cacctcgaac
       61 ctcttcgcaa agacttggct ctttattaca ccggaaattt agaactcagc cccgagcata
      121 aacaaaagat tgaagtggct ccttatatca cggctgagtt tcgcgtgaag aatgacaaag
      181 ttcatggcac gctcttgcgg ggctacgttt accagaaact ccttgaattc aaaggagaat
      241 ctcttaaaga acagacacgc ctgctttacc ccatcaagcg cattgaacaa tttttcgtag
      301 atcgccgcac agacccttac tatcaggatt tgattagcca gctcgaaaga accctggcgc
      361 tcattcaaca aggcgaccga gaagcgttaa actggagctc gatgattctg aatcaggccg
      421 agaccgctct ggaaaacgtt ttcacgggtg acaaacttct tactcttctg gtaagagatc
      481 tgcgtcatac tagtgaatct actcgcaaat taaatccgtc tgcaaagatg gaccctgcga
      541 tcaatgaggc ggatgaagaa tgtctgaaaa cagctcccac aaagactcca acaataactc
      601 cgaaaccaaa ttacgactta acaaattaat cgcggacagc ggacttgcca gtcgccgtca
      661 cgcggatcgg atgattgaag aaggtcaagt gaccgtcaac ggcaaacgag tctacgagtt
      721 agggattaaa gtcgatcccg ctcatgatca cattttagtg aatggcaaac cgctcaaaaa
      781 acctcttgaa aacatctata ttctcttcca taaacctaaa ggcgttttaa ccacgatgga
      841 agatcccctc ggtcgcccca ccatcgcgga atatctcgat ggcgttccgg ggcgcgtatt
      901 ccctgtgggt cgtctggatt gggactccga aggaatgttg cttctgacga atgatggcga
      961 attcgctaac aaagtcactc atcctaaagc ggaagtcaca aaaacctata tggtcaaagt
     1021 tgatggccag cccaccaagg aagagattca aaaactagtc cgcggcgttt ccatcatcgg
     1081 cggtaaagtg gcggctaaac acatcgaaaa aatcaaacgc caagaagggt ccgacaaata
     1141 cgattggtat aaaatggtga tcacagaggg taaaaaccgt cagatccgtc agatgtttga
     1201 aaaaatcggc tacgatgtca tgaaacttca gcgggtggcg atcggtcgtt tgcgtatcgg
     1261 tgccgtcgag cgcggagagt tggttttcct aaacgatgcg gctgttgagc gagtcttctt
     1321 gccggacgct ccggatgaaa tcaaactcaa aaagacttac aaaggtcgcg cgattgcgca
     1381 aagaattgcg gcggggaaaa ctgctgacgg aaaatcatct gcccgcaaag cgaagactgg
     1441 atcaaagcct ggagtaaaag ctggagtaaa atcaagatcc aagacaggct ataaacctaa
     1501 aaagtctttt aataaataaa agtgaaccgc aagcaacttg ttggaatttt tgaaatcact
     1561 ctggggagca ttggctttgc ttttctgggg attttcggca aatgggcttt tagcagcggc
     1621 cttagtgtgg gcgcactcct tacctatcgc tttattatag cctcgatggc tttatggatg
     1681 ggtctctttc tttttaatcg cgacaagatt tatatttccc gaactcagct tctgatctct
     1741 gcaggtttag gggtttttgg ctatgcagtg tttgcgactc tttatttttc cgcagtccaa
     1801 ggcatgagcg tcggccttgc cgccttattg ctttatactt atcccttttg ggtcagtctg
     1861 ctcgggcatt tcttcttgcg tgaacgaatg aatcaaaccc agtggctttt gttgttaggt
     1921 gctttcttgg gtgtcacttg ccttttatgg ggacagtttc atcttaacaa tatgaaagcg
     1981 tttattgctg gtttgggctc agctatcact tatgccatct atattttatt ctcggcacat
     2041 tatcaaaaaa atgtgaagcc gctctcctcc agtctttatg tgactacttt cgcagccatt
     2101 gccttagcga gctatcacca tcctcctttg caattctgga aaatcttgga ctttcagcag
     2161 tggcaaatag tcctcggaat tgccacagtc tcaacgatct tccctatgac tttaattcta
     2221 ggtggactgc aaaaattaaa aagctcccaa gcggccctca tcaccatgct cgaaccggtg
     2281 attgccgcca ttgccgctgg aatcctgctc cacgaaagca tgagtggctt gcaaatcgtg
     2341 ggcgccactc tagtcatgac gtgtttgggt ttgagtgtcc tccgtgccca ttagccgagg
     2401 agccacttcc aaacaggcat gacaacgaca ccatctataa cgtcttcata atcataggtt
     2461 agcaatagcg gctgcccata acccaattca tttttacctt ggaataaacc attgaattca
     2521 cgtgtgcgtg ttttcatatc tgaaatatcc caactcactt ggatgagatc ctggattttt
     2581 ctattttttg tacagataaa atcaacttca aaatttttat ttttgaaaaa tgaaatttct
     2641 ttttttcgtc ttttcagttc aagaaaaacc aaattctcca actgccgacc cagggttctt
     2701 tgcggattct ctgacaacgc attaaaaagg cccgtatcaa tggcataagt ctttcgactc
     2761 ttggtcaaac gctctcgaat tttcggagag taagactcaa cttcaaagca taaataagtt
     2821 tcttgaatgt ag
//
LOCUS       DATBTE010000245         4936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1671423, whole genome shotgun sequence.
ACCESSION   DATBTE010000245 DATBTE010000000
VERSION     DATBTE010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671423"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="VIG33_08985"
     CDS             complement(<1..170)
                     /locus_tag="VIG33_08985"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEY8271011.1"
                     /translation="MNKYLIFASMGFELVGIMLASIYLGQLIDKTYQTKGFGLIVLMF
                     FGLVSWLTHVVLL"
     gene            complement(170..919)
                     /locus_tag="VIG33_08990"
     CDS             complement(170..919)
                     /locus_tag="VIG33_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8271012.1"
                     /translation="MPPEAKDYKEAQKEMAQGHHRIALSSIDRVMKRAPESEYAVKAA
                     RDGARIASLEIKDYKRALEYYQFLVLHSKDSGERVSAQKQLASIYFDQLQNYDKAIIE
                     LNKLIRDTESDVDIARNKLDIARANYYQNNLFQAQSELDDLLKLHIDDNERFSAWVLK
                     ANIHIALKEYPKAIEVLRKVTATYPQKSVQENVYQTLAVCYEENDNFTEAIKILESVK
                     DKYTKPEYIEIRIKRLKERQKNQPGARGLRK"
     gene            complement(971..3436)
                     /gene="gyrA"
                     /locus_tag="VIG33_08995"
     CDS             complement(971..3436)
                     /gene="gyrA"
                     /locus_tag="VIG33_08995"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEY8271013.1"
                     /translation="MNNTQEKGVTLVDINKEMREAYLQYSMSVIVGRALPDVRDGLKP
                     VHRRILFAQHELSNTHDKPYKKSARVVGDVIGKYHPHGDAAVYETMVRMAQNFSLRYP
                     LIDGQGNFGSIDGDNAAASRYTEVRMTRLAEELLNDLDKETVAFGPNYDDSLQIPLVL
                     PAKYPNLLVNGSSGIAVGMATNIPPHNLGEVIDGCIHVINNPDCTIDDLMAHVKGPDF
                     PSAGVIAGREGILQAYKKGRGVISLKAVAEIVPGKDKDREEIIISEIPYQVNKAKLIE
                     SIADLVRDKQIEGIADIRDESSREGMRIVIILKRQENASVILNRLYKFTQMQVSFGII
                     MLALDAKNQPVTFNLKNMLEAFIDHRRDVVTKRCIFEIKKAQERAHILEGLKKALDHI
                     DEVIALIKASKEANTAREALIEKFEFSERQAQAILEMRLQRLTGLEREKILAELEALI
                     KEINWLKMVLADVREIYKIIMAELEDIKKRYADSRRTEITGDLTDIEDEDLISDESMV
                     VTITNTGYIKRIPTEEYRVQKRGGKGLKGMETKEEDYVNNIFTASTKTMLLVFTDKGK
                     VYWCKVHKLPLGNRTSKGKAIANVVQLSSNEKVRSILPIDQFEENKYIVMLTERGIIK
                     KTSLDSFSNPRTAGLIALTTDLDDAIIDCKISDGSSDVFIATKEGMSIRFNENDVRGM
                     GRTARGVKAMTLAKDDIVIGMEVFEKGNKDTILMVTQSGYGKRSELEEYRIQSRGGVG
                     IITQKTTDKVGLVVGTRKVKATQEVILSTDKGQNIRMKVSDISVLGRNTQGVRLINLS
                     EKEEKVTSLAVVDDEDVAGEEIH"
     gene            complement(3464..>4936)
                     /locus_tag="VIG33_09000"
     CDS             complement(3464..>4936)
                     /locus_tag="VIG33_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEY8271014.1"
                     /translation="EDIREGLAAVISVKVREPQFEGQTKSKLGNTEVKGIVESLVNEK
                     LSDWMDRNPSVAKNIVMKCVEAARARDAARKARDLTRRKTALDGGSLPGKMADCQERD
                     PALCELYLVEGDSAGGSAKQGRDRRTQAVLPLKGKILNVEKARFDKMLSNEEIKMMIS
                     ALGTGIGKDNVNVDKIRYHKIIIMTDADVDGSHIRTLLLTFFYRQMPDVLERGYIYIA
                     QPPLYRAKKGPAEQYLKDESALTEYLLSSGLGNFKIKGKENATEVDLRKMILNIQKFY
                     NLLRVSSAKYDKDVLYFFLSRVMDLEKTLNSEPKLSAALKELELWIRANPKLGVTELN
                     TEVKTDPETNKIYAEIYSVRYADRKTTRISESILNASEVIELKNLWASIQGVTTLPLM
                     ITQGEQEYQFENYNEFHQHVMEMTKKGVYIQRYKGLGEMNPEQLWDTTLNPENRALLR
                     VTIDDAVAADETFSVLMGELVEPRRQFINENALFVRALDV"
BASE COUNT         1373 a         1117 c          881 g         1565 t
ORIGIN      
        1 aggaggacga catgggttag ccagctaacg agtccaaaaa acatcaatac gattaatcca
       61 aatcccttgg tttgataggt tttatcaata agctgtccta aataaataga agcaagcatt
      121 attccgacaa gttcaaagcc catggaagca aaaataagat atttattcat tatttcctaa
      181 gccctcgcgc acccggttga tttttttgcc gctcctttaa gcgtttaatt cgaatttcaa
      241 tatactccgg tttcgtatac ttatctttta cagattctaa aatctttata gcctcggtga
      301 aattatcatt ctcttcatag catacagcca gggtttgata aacattctct tgcacagact
      361 tttgcggata agtagctgtt accttcctga gaacttcaat tgctttggga tattctttca
      421 aagctatatg aatattagcc ttgagaaccc aagccgaaaa tctttcatta tcatctatgt
      481 gaagcttcaa gagatcatca agctcactct gagcctgaaa aaggttattt tggtaatagt
      541 ttgctcgggc gatatcaagt ttgttacgtg caatatccac atcagattca gtgtcacgaa
      601 tcagtttgtt taattcgata atggctttat cataattttg taattgatca aaatagattg
      661 aagctaactg tttttgagca ctcactcttt cgccagagtc tttagagtga agaacaagaa
      721 attgataata ttccaatgct cttttgtaat ctttaatttc aagagaagcg atacgagcac
      781 catcgcgtgc cgccttaaca gcatactcac tctctggagc tcttttcatg actcgatcaa
      841 tacttgaaag cgcaatccga tgatgaccct gagccatttc tttctgcgct tctttataat
      901 ccttagcctc aggaggcacg caggcgtata gaccgagagt tatccctacg atcaaaacct
      961 gcttgcgcat ttaatgaatt tcctcaccag ctacatcttc gtcgtctaca acagcaagac
     1021 ttgtgacttt ttcttctttt tcagaaagat taatcaagcg gacgccttgt gtgtttctcc
     1081 ccagaacaga aatatcagac actttcatgc gaatattttg acccttatcc gtcgataaaa
     1141 tcacttcttg agtagctttt actttacgag tgcccacaac taagcctact ttatctgtag
     1201 ttttttgcgt gataattcca acgcctccac gactttgaat gcgatactct tccagctccg
     1261 aacgcttgcc ataaccagac tgagtcacca tcaagatagt atctttattt cctttttcaa
     1321 agacttccat cccaataacg atatcgtctt tcgcaagagt catggctttc actccacgtg
     1381 cagtacgacc catacctcgc acatcatttt cgttaaagcg gattgacata ccttctttgg
     1441 tagcaatgaa tacatcactg gagccatctg aaattttaca atcaatgatg gcatcatcta
     1501 gatctgtagt gagagcaata agccctgccg tgcggggatt cgagaaagaa tccaaagacg
     1561 tttttttaat aattccacgc tctgtaagca taacaatgta tttgttttct tcaaactgat
     1621 caattggaag aatggatcgc accttttcgt tcgaagagag ttgaaccacg ttagcaatcg
     1681 cttttccttt cgaagtgcga tttcccaacg gaagcttatg tactttgcac cagtaaactt
     1741 ttcccttgtc cgtaaagact agaagcattg tcttggtact agcggtgaag atattattta
     1801 cataatcttc ttctttggtt tccattccct taaggccttt acctccgcgt ttttgcacac
     1861 gatactcttc agtaggaatg cgtttaatat accctgtatt ggtaatagtc actaccatgg
     1921 attcatcaga aattaagtct tcatcttcaa tatctgtcaa atcgcccgtg atttctgttc
     1981 ggcgagaatc tgcatatctt tttttgatat cttcaagctc cgccatgata attttataaa
     2041 tttcgcgaac gtccgcaaga accattttaa gccagttgat ttctttaatg agagcttcaa
     2101 gttcagccaa gattttttct ctctcaaggc cagttaaacg ctgcaagcgc atttcaagaa
     2161 tagcttgcgc ctgacgttca ctaaattcaa atttttcaat gagtgcttct cgagcagtat
     2221 tcgcttcttt tgaagcttta ataagggcga tgacttcatc gatatgatcc agagcttttt
     2281 taagcccctc taaaatatgc gcacgctctt gagctttctt aatttcgaaa atacatcgct
     2341 tagtgaccac gtcacgacga tgatcgatga aggcttcaag catattttta aggttaaagg
     2401 tcactggttg gtttttagca tctaatgcta gcatgatgat accaaagctc acttgcatct
     2461 gcgtgaactt gtagaggcga tttaaaatca cgcttgcgtt ttcttgacgc ttaagaatta
     2521 tcacaatacg catgccctca cgtgaagact catcacgaat atcagcgatc ccttcgatct
     2581 gtttatcgcg aacaagatct gcaatacttt ctatcaactt agccttattt acctgataag
     2641 gaatttcact tataatgatt tcttcgcggt ctttgtcttt tcctggaaca atttcagcta
     2701 cagcttttaa ggaaataaca ccacggcctt ttttataggc ttgaagaatt ccttcacggc
     2761 ccgcaataac accggcagat ggaaagtctg gccctttcac atgggccatt aaatcatcaa
     2821 ttgtacagtc tggattattt ataacatgaa tacatccgtc gataacttcg cctaaattgt
     2881 gaggcggaat atttgtcgcc ataccgacgg caatccctga cgaaccattt accaaaagat
     2941 ttggatattt cgctggaaga acgaggggaa tttgcagaga atcatcgtag ttcggtccaa
     3001 aagcgaccgt ttccttgtcg agatcattaa gtaactcttc cgcgagacga gtcatacgaa
     3061 cttctgtata acgagaagca gcggcattat ccccatcaat ggaaccaaaa ttcccttgac
     3121 catcaatcaa agggtaacga agagaaaaat tctgggccat acgaaccatt gtttcataga
     3181 ccgcagcatc tccgtgtgga tggtatttac ctataacgtc accaactaca cgggccgatt
     3241 ttttataagg cttatcgtgc gtattactaa gttcatgttg agcaaacaag atgcgtcgat
     3301 gaaccggctt taaaccatca cggacgtcgg gcaatgcacg accaacgatt actgacatcg
     3361 aatattgaag gtatgcttca cgcatttcct tatttatgtc gacgagagtt acaccttttt
     3421 cttgagtatt attcatgatt caccaattca aattattttc taattaaaca tccaaagcgc
     3481 gaacaaacag ggcattttca ttgataaatt gacgtcttgg ttcgaccaat tcgcccatca
     3541 atacgctgaa ggtttcatca gcggcaaccg catcatcaat ggttactctt aaaagcgcac
     3601 gattttcagg attcaaagtc gtgtcccaaa gttgctctgg gttcatttcg cctaatcctt
     3661 tatagcgctg aatataaaca cctttcttag tcatctccat cacatgctgg tggaattcgt
     3721 tataattttc gaattgatac tcttgttccc cctgagtgat catcaaaggt aacgttgtca
     3781 ctccctgaat acttgcccaa agatttttta actcgataac ttcagaggcg tttagaatgc
     3841 tttcactaat tctagttgtc ttgcgatcgg cataacggac ggaataaatt tcagcataaa
     3901 ttttgtttgt ttcagggtca gtcttcactt ctgtatttaa ttcagtgact ccgagctttg
     3961 gatttgcgcg aatccaaagc tcaagttctt taagagcagc gcttaacttc ggctcgctat
     4021 ttagggtttt ctccaaatcc atcactctac ttaagaaaaa gtatagaaca tctttgtcat
     4081 attttgcgga agacactctc aaaagattat agaacttttg aatatttaag atcatctttc
     4141 gcagatctac ttccgtcgca ttttcttttc ctttaatttt aaaattacca aggcccgaac
     4201 ttaaaagata ttccgtaaga gcggattcat ctttaagata ctgttctgct gggccttttt
     4261 tagctcgata gagtgggggt tgggctatat aaatatagcc gcgctcaagc acatcaggca
     4321 tttggcgata aaagaaggtt aaaagtaagg ttcggatatg tgatccatcg acatctgcat
     4381 ctgtcatgat aataattttg tgataacgga ttttgtccac attcacattg tctttaccaa
     4441 ttccagttcc caaagcagag atcatcattt taatttcttc attagaaagc attttatcaa
     4501 aacgtgcttt ctctacattt aaaattttcc ccttcaaagg caaaaccgct tgcgtgcgac
     4561 gatcgcggcc ttgttttgca gatccacccg ctgaatctcc ctccaccagg tacaattcac
     4621 aaagagcagg atccctttct tggcagtccg ccatttttcc aggcaaagat cccccatcaa
     4681 gagccgtctt acgacgagtc aaatcacgag ccttacgggc agcatcgcga gctcgtgcgg
     4741 cctccacgca tttcataact atattcttgg ctactgatgg gtttcgatcc atccaatcag
     4801 aaagtttttc atttaccaga gattctacaa taccttttac ttctgtattc ccaagtttgg
     4861 actttgtctg tccttcaaac tgaggttctc gaacttttac agatatgacc gccgccagtc
     4921 cttcacggat atcttc
//
LOCUS       DATBTE010000246         4254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_989118, whole genome shotgun sequence.
ACCESSION   DATBTE010000246 DATBTE010000000
VERSION     DATBTE010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989118"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VIG33_09005"
     CDS             complement(<1..331)
                     /locus_tag="VIG33_09005"
                     /inference="COORDINATES: protein motif:HMM:NF028503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiEi antitoxin N-terminal domain-containing
                     protein"
                     /protein_id="HEY8271015.1"
                     /translation="MGVGITNKLNILLNEWPVGEIYSTALLHKKGYSDQQIKNYLDAG
                     WIERLGIGAYKKANDQIEWPAGLLVLQEQLKLPVHIGGKAALALLGKSQYLELGEINL
                     ILYGPKND"
     gene            479..555
                     /locus_tag="VIG33_09010"
     tRNA            479..555
                     /locus_tag="VIG33_09010"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:513..515,aa:Pro,seq:ggg)
     gene            686..1813
                     /locus_tag="VIG33_09015"
     CDS             686..1813
                     /locus_tag="VIG33_09015"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8271016.1"
                     /translation="MERYLSSHLRLLLKKKLILLMGPRQVGKTTLSKSLVKEAAYYNY
                     DIKKDLQVFMRQEWDRSKELVIFDELHKMKKWKLWLKGLYDEGFLEKQKVLVTGSARL
                     DIAKKMGDSLAGRFFSLRLNPLDLKELKGRNSAEKNYRKLLLCGGFPEPFFEGSEKFY
                     GLWKRTHTDLILRQDMITLENIRDLDGVETLVELLTQRVGSTISIHSLAEDLDRDDKT
                     IKHWLQVLENLYIVFRVPPKAKNIARGIKKSSKYYFFDIARVENGEAAQLENLVALSL
                     KKELEFLEDTEGLGFSLSFAKTKDDKEIDFVIERPKKKPLLIEVKLSDANPAKNFDLF
                     RRFFPQAECIQLVRQLDREFSTPSGTKVMSALPYLENLNLL"
     gene            2036..2260
                     /locus_tag="VIG33_09020"
     CDS             2036..2260
                     /locus_tag="VIG33_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271017.1"
                     /translation="MQVGKLKAENNKILESHEVYGFSNKTKMIDYAMDLLRERMKKEK
                     RRTEREEMLNLYAQSSPESYFAEIDGEDFE"
     gene            2253..2606
                     /locus_tag="VIG33_09025"
     CDS             2253..2606
                     /locus_tag="VIG33_09025"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEY8271018.1"
                     /translation="MSNFSVGEVVLLDLDPSKGHKQKKTRPCVVINVHPRLDLLTVFP
                     ITDSKGKSGKVFIEIPDLNSAGLTKPSVIDTYQIRTLSTQRIIKKLGKISDDNIFDCR
                     KCIALIYEIDEEHLA"
     gene            complement(2666..4186)
                     /locus_tag="VIG33_09030"
     CDS             complement(2666..4186)
                     /locus_tag="VIG33_09030"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8271019.1"
                     /translation="MNNSRRKFLAKMLMGTTGLMARNSFADILRSSKLSSQIEKCDCI
                     VIGAGMAGLIAARDLSFPKYARRGYKTIVLEASGRIGGRILTMEDPRFGGPLEMGAEH
                     IHQEPGSVALWNELDRYSPKLLKIPRMLKGLMYYDGWEDNLKNHYSLWKEWNIWDIAT
                     FSQRIDAYQGPDISAKEWIERQKFDNIGKNLVDLYFTGGVPGPLDKISIKGFTADRIS
                     ELELGWNEYQFVEGYSKFLDQLTQGVDQHTGKELDIRLRSVVKYIKYGAQGTEVHTAD
                     GRIFLAQSVIITASVGMLKSGEITFDPSLPQDKMDALNCMGMGDEAKVTLKFKKRFWP
                     DDAVFLNRIDNYREMGRTCYIPFADDPEKNKVLAVLMAGDEADKIKNMRDIEVIRALC
                     RDFDRMFPKAAPTFNLLARDGAAQDITYLRWQWSQDPFSKGADSFLKVGSEKSVPVEK
                     ARKVLAHPASTPSLFWAGEATACSEYTQPCSTHGAHFSGARAAIEAGEFLRRKLVV"
BASE COUNT         1222 a          910 c          913 g         1209 t
ORIGIN      
        1 gatcattctt aggcccataa agtataaggt ttatttcccc taattcgaga tattgacttt
       61 ttccgagaag cgccaacgcc gcctttccac cgatatgaac tggcaattta agttgttcct
      121 ggagtactaa gaggccagca ggccattcta tttggtcgtt ggctttctta taagcgccaa
      181 tccctaatcg ctctatccaa ccggcatcga gatagttctt tatttgttga tcgctatagc
      241 ctttcttatg cagtaaagcc gtagagtaga tttcaccaac tggccattcg tttagaagta
      301 tgtttaattt attcgttata cctacaccca taatgtagtt ataccaaata aactaaacta
      361 aactaaacta aactaaacta aaccaaattc tcactttaag tcagaagaga acaaatggat
      421 aattctagac attcacggaa gctatagtta tttattaagt gttcatccat cacttaatct
      481 gcgcgtggct cagcttggta gagcacttgc ttggggtgca agaggtcgct ggttcaaatc
      541 cagtcgcgca gaccagtttt tttgccctgg cctctaaaat tcgaacaaat tggtcgtctt
      601 ctagttgcct gagtgggtgt ggcctttttg gagtgtactc cgaaaaggcc ttgacttttt
      661 tggagttttg tccgaaaaag acattatgga gcgatatctt tcttctcatc tcagactctt
      721 gttgaaaaag aagctcattc ttttaatggg accccgtcag gtgggcaaaa ccaccttgag
      781 caaaagcctg gtcaaagaag cggcctatta caattatgac atcaaaaaag acctgcaagt
      841 ttttatgcgg caagagtggg atcgctcaaa agagctggtg atttttgacg aacttcataa
      901 aatgaaaaaa tggaagctct ggttaaaggg cctttacgat gaaggttttt tagaaaaaca
      961 aaaggtcttg gtcacgggga gcgcgcgatt agatatagcc aaaaaaatgg gagattcgtt
     1021 ggccgggcgg tttttttctt tgcggctgaa tccgttggac ttaaaagagc ttaagggaag
     1081 aaattcggca gagaaaaact atcgcaaact cttgttgtgc ggcggatttc cggagccatt
     1141 ttttgaggga agtgagaagt tttatggtct ttggaaaaga acccatacgg acctcattct
     1201 ccgtcaggat atgatcacgc tcgaaaacat tcgggacctc gatggcgttg aaacactggt
     1261 tgaattattg acacagagag tgggctcgac aatatctatt cattcattgg cagaagattt
     1321 ggatcgcgat gataagacga tcaagcactg gctacaggtt ttagaaaatc tttatattgt
     1381 gtttcgagtt cctcccaagg caaaaaatat cgctcgtggc atcaagaaat cgagcaagta
     1441 ttattttttc gacatcgctc gcgtagaaaa tggcgaggcg gcccagcttg aaaacctcgt
     1501 ggccctcagt cttaagaaag aactcgaatt cttagaggac acagaaggtc tcggattttc
     1561 tttgagtttt gctaagacca aggatgataa agaaatcgat tttgtgatcg aacgtcctaa
     1621 aaagaagcct cttctcatcg aggtcaagct gtcagacgcg aatccagcga aaaattttga
     1681 cctattccgc cgctttttcc ctcaggctga atgcattcaa ttagtccgac agctggatcg
     1741 agagttttcg actccgagcg gaaccaaagt gatgtcggcg ctgccatatt tggaaaacct
     1801 gaatttgctt tgagtctaat gtcgaactcg gtcagctcta agttgcggta ttagagtaga
     1861 cccttctcta aagcctgatc ttaagcccga tattgaatag aagttgttta atttcaatga
     1921 cttacaaaaa aatattgtgt atgtacgtat ttatgtatat aataacgtat gtatttacat
     1981 ctaaatttaa gtacttgata gcatatgcca tatatacgtt aacatgaggt agattatgca
     2041 agtaggaaag ttaaaggcgg aaaacaataa gatactggag agtcacgaag tctatgggtt
     2101 tagcaataaa accaagatga tagactacgc catggatcta ttgcgtgaaa gaatgaaaaa
     2161 agaaaagcgc cgaacagagc gtgaggaaat gttaaatcta tatgcgcagt cttcaccaga
     2221 gagttatttt gcggaaatag atggagaaga ttttgagtaa tttttcagtt ggtgaagtag
     2281 tactgttaga tttagatccc tccaaaggac acaagcaaaa aaaaaccagg ccgtgcgttg
     2341 taataaatgt tcatccgaga cttgatctgc tcacggtctt tcccattaca gattctaagg
     2401 gaaaaagcgg taaagtattc atagaaatcc cagatcttaa ttccgcagga ttgaccaagc
     2461 cctcagtgat agatacctac caaatccgga ctctttctac tcaaagaatt ataaaaaaat
     2521 tagggaagat atctgatgac aatatttttg attgccgaaa gtgtattgca ttaatttacg
     2581 aaattgatga agagcatcta gcgtaatcta tgtggggata accaacctgt tctgctaagt
     2641 ttagaacact gtgttataaa tgtctctata caaccaactt cctccgcaaa aattcgcccg
     2701 cttcaatcgc cgctcttgcg ccggaaaagt gcgcgccgtg ggtggagcaa ggttgggtgt
     2761 attcactgca cgcggtggcc tcgcctgccc aaaacaagct gggcgtagag gccggatgag
     2821 ccaatacctt tcttgccttc tccacgggga cggatttttc agagcctact tttaaaaaag
     2881 aatctgctcc cttagaaaaa ggatcctggc tccattgcca tcggagataa gtgatgtctt
     2941 gagctgcgcc gtctcttgcg agaagattaa aagtaggggc cgccttggga aacattctat
     3001 caaaatcccg gcagagggcg cgaatcactt cgatgtccct catattttta attttatcgg
     3061 cttcatcacc ggccatcaat acggcaagca ctttattttt ctccggatca tcggcgaaag
     3121 gaatatagca agtcctgccc atttcgcggt agttatcaat gcgatttagg aaaacggcat
     3181 cgtcgggcca aaatctcttt ttgaatttta aagttacctt tgcttcatcg cccatgccca
     3241 tgcagttcaa agcatccatt ttatcttgtg gtaaagatgg atcaaaagtg atttcaccgc
     3301 ttttaagcat gccgacagag gcggttataa taaccgattg agctaaaaag atccggccat
     3361 ccgcagtgtg aacttcagtt ccctgcgctc catattttat gtatttcacc accgagcgta
     3421 agcgaatatc taattctttt ccggtgtgtt gatccactcc ttgagtcagt tgatctaaaa
     3481 atttgctgta gccctccacg aattgatatt cattccagcc cagctccagc tctgaaattc
     3541 tatccgcagt aaagccttta atactaattt tatccagagg ccctggtacg cctcccgtga
     3601 aataaagatc caccaggttt ttaccgatat tatcgaattt ctgccgctca atccattcct
     3661 tcgcggaaat atccggccct tgataggcat caatccgctg agaaaaagtg gcaatgtccc
     3721 aaatgttcca ctctttccac agggagtaat gattcttaag gttgtcttcc caaccatcat
     3781 aatacataag tccttttaac attcggggga tctttagtaa ctttggactg tagcgatcca
     3841 actcattcca gagagctacg ctgccaggct cctgatggat atgttcagcg cccatttcta
     3901 gaggaccgcc aaaacgtggg tcttccatag taagaatacg cccaccgatt ctgcctgacg
     3961 cttctaggac aatagtttta tagcctcttc tggcgtattt cggaaaactc aaatccctgg
     4021 cggcgatcag acccgccatg cccgcgccaa tgacgatgca atcgcatttc tcaatttgag
     4081 acgatagttt tgaagaccgc aaaatatcgg caaaactgtt tcgcgccatc agccctgtgg
     4141 ttcccataag catcttggca agaaacttgc gtcttgagtt attcatatgt ctcaacctcc
     4201 gaatttagaa tcttttaatg cgagagtcgt accaaggcgg ttgagcattg tatt
//
LOCUS       DATBTE010000247        15117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_374986, whole genome shotgun sequence.
ACCESSION   DATBTE010000247 DATBTE010000000
VERSION     DATBTE010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 15117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15117
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374986"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VIG33_09035"
     CDS             <1..515
                     /locus_tag="VIG33_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEY8271020.1"
                     /translation="PAVSGGLNILKFDYDGIHQELLQVESSPLAQHFLLVYTGRTHHS
                     GMNNFEVMKGAVAKDPTTLKALKDIKDIAERMEKVVRESCWSELPGLFREEFTARVRL
                     APAFTSPEIEKLNELSLQNGAHAVKICGAGGGGCVLIWTPEEKKEGVAKACQKAGFQV
                     LATRPVNPLL"
     gene            458..1336
                     /locus_tag="VIG33_09040"
     CDS             458..1336
                     /locus_tag="VIG33_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEY8271021.1"
                     /translation="MSKSRIPSSRHSSRESTPVGDVIRFVGLSLGGGKADKACMAILE
                     YYPKHHKVFLAKIFDRIKTEEAISADLKIHEILDQYHGMIHSVALDTPWSLPTCLRCK
                     LKCPGYENCQEPHIQWMWDYVKEKNAKKKPKKLFTPYTQRAVEMHLATGFDEPFQIPN
                     AMGANSAPLLARAHFILRRLDIQTLEVFPKLSIWRIGLSLHILKSNLRSHKHSAVGED
                     SRKGILKELGEHNIAFVYDQDRRLMIENNHAFEAFICAMTAFLEYKGQTEARPRGFPK
                     NEDWISFPKEKINWKF"
     gene            complement(1464..2393)
                     /locus_tag="VIG33_09045"
     CDS             complement(1464..2393)
                     /locus_tag="VIG33_09045"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEY8271022.1"
                     /translation="MKLFTQASAAIVLVTLAVGCTPANKNNMNSSSKTSSIIGGTEVP
                     EGAALQKSIVGIYDMKKGALCTGSLLENNIVLTAAHCIGAAAEDLLIVFSTDLPGVFQ
                     SQDKNLILQKLRRGVKTVVNPNWGKTSQVPNAATGDTALIKFAGPVPAGFKPATLLNS
                     AEVLTEGLTITVAGYGVSKDVLTEVNKDEIPDFQEKVLKGEIFCETSKAGEIGKCYKE
                     EASGEGYLRTTELQVAGDFNDTEIVFDQQHGQAACEGDSGGPAYVKQANGEYHLFGVT
                     SRGTLGCDGFIVYSNITSKKLSAWLTYAMEQLK"
     gene            2599..3675
                     /locus_tag="VIG33_09050"
     CDS             2599..3675
                     /locus_tag="VIG33_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HEY8271023.1"
                     /translation="MAEWESRSEINSDVVFFPYVPVGLEKNSEEVFLWDLDKTYLDTT
                     IDSLRGLLQTILERAWNKRNIPGTSTLIRALSIYRAKKSGQKYFPIYFITASPPQMEE
                     RISEKFSIDEIHPFGCFYKDNLKNLRPGRFWRLTKQIGYKLQALMQLRTRLHPQVRQI
                     CWGDDSESDAVIYNLYSDICARRIGPHEIRNILEKLHVTGEQVDQILVLQSQIPENDP
                     VEKIYINLATDTDPDYYLKFGRRTLATYNTYQVALDLVQDQRLSFEGLYAVAQDMIYN
                     YGFSPEELMQSFDELIRRGVLGQTCYEKVRPFLEEKGLIFRDFEPSMAPLKEKHVIDG
                     KVFEMEGVHEPWVHERVDYLHESR"
     gene            complement(3672..4502)
                     /locus_tag="VIG33_09055"
     CDS             complement(3672..4502)
                     /locus_tag="VIG33_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271024.1"
                     /translation="MKSPVNDKPRTVHFLYSCSALKGLPIGTFLLVILLSFATHALPE
                     NIENYEVLPGGLTDIQDGAYVEGSVRNRGIQPDYQIDLQSEPLKSLLVAAREIGLQNI
                     SYWDKIGLIVELMRTDFFKYHNYHNPYYRRLLKKYRLAKKDVPLSEYGVCGAGVCREH
                     ALVLHFALKAAGIDNKHAYAEIYRASNYHGYQIIEDHAFTVVKHEEVDWVVDAYYWGF
                     NGFRLQDLLSSSGITEHSPHAPIAAPGPGTRWIVKINNFPKIYNPKFKASLCAGVFLH
                     "
     gene            complement(4567..5370)
                     /locus_tag="VIG33_09060"
     CDS             complement(4567..5370)
                     /locus_tag="VIG33_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY8271025.1"
                     /translation="MAKNKWLIPSLVIAGLFFVGLSWFMSARLENQSSGLKVLARVIY
                     NSGEVYVLHKNITEKETLKKSANLYYLDTVETGPNGDASFELNSGYRLRVLDNALITL
                     DQEGEKAIFILKRGDVQIESFGRGSALMISKNGQRLSPSEYESILRKENPQNTFPEIA
                     PVVADSPPTPASADSLSSEYIQETLKRQIPAFDKCYKQLLQRTPGVVGQVVMTFTIER
                     SGKIASAGVTNSTISNAEFKRCLSEALRRIEFNSFRGDPITSTFPVSFE"
     gene            complement(5410..5754)
                     /gene="secG"
                     /locus_tag="VIG33_09065"
     CDS             complement(5410..5754)
                     /gene="secG"
                     /locus_tag="VIG33_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY8271026.1"
                     /translation="MGTFIAVLHVIVALVLISLVLIQDSKGGGALGMGSGGGSNSVLG
                     ATGASTLAAKMTRIAAIVFALTSIGLTVLSARESKSILDKLPATSAIPAATPATPAAA
                     PTTSAPTQAPAK"
     gene            5895..6536
                     /gene="tmk"
                     /locus_tag="VIG33_09070"
     CDS             5895..6536
                     /gene="tmk"
                     /locus_tag="VIG33_09070"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY8271027.1"
                     /translation="MAFLVFEGLDGSGKSSLMRALEAELSKLGISFHRTREPGGTLLG
                     DEIRNMILRKQEGTTPVPRAELLLYEASRAQHVDEIIKPKLAQGHWVLSDRFAASSVA
                     FQAGGREVQEKDVVVLNHFATDGLKADLTVLLDLSVEDSRKRQVQRTSQTGEAADRIE
                     SEKDSFHERVRLSFLKQAEDDRQGWLVLDARSSTEEMLKILLKTLRERKWLLP"
     gene            6521..7492
                     /locus_tag="VIG33_09075"
     CDS             6521..7492
                     /locus_tag="VIG33_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HEY8271028.1"
                     /translation="MASSMNRLLDQVFGHKEVISKLLATFTGERPGQTFLFVGPHGVG
                     KKKVALGFAQALLCDTLRTACGVCGSCRRVAAGTHESLKIIASEGPQIKIEESREVIQ
                     FLNLQGITQRRVVVIDQVQTLNLQAANSLLKTLEEPPPETYFFLIAPSVAGVLSTIRS
                     RSRVVSFHPLAAEEIARGAGSAPTWTIRASQGSFEKLARLQEGPEQELRREALSLLQQ
                     FLEDKEFLTADNWRDSFKERVKSQRILSYWISFLRDVLYFQEDQKNSIMNVDQVDFLK
                     SMSERSRNQILELIEKALTVEKEIFQNRDVQLLMEEFWILQNRGGEV"
     gene            7494..8267
                     /locus_tag="VIG33_09080"
     CDS             7494..8267
                     /locus_tag="VIG33_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY8271029.1"
                     /translation="MLEWMDLHAHLNMLEEGPEEALRLAREAGVVKVATIGTEPQDLP
                     LVLELAEKNYPNVFCTLGIHPHEGQIYTAEIGQFIENNVSKPWVIAVGEIGLDYYYEH
                     SSHEEQKQAFRAQLEIAARTQMPVQIHTRDADEDTIEIMKEFKGGVKGIIHCFTGTPW
                     LAEQALALGYNISISGVVTFKSAEALRAIIRTLPLDRIHVETDAPFLAPIPMRGRKNT
                     PAFVTHTAKVVADLKGVSLEELAEITRKNALKMFPKIIW"
     gene            8287..8988
                     /locus_tag="VIG33_09085"
     CDS             8287..8988
                     /locus_tag="VIG33_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY8271030.1"
                     /translation="MSLKLLFQDEHFIAVDKPSGFHVHPHEDKRNRISRDKVCLYLAR
                     DMMRRHVYPVHRLDAGTSGVLLFALSSASASVLCKLFAEKSLQKTYHAVVRGYTPEQG
                     NIELPLESDSTGDLVEAKTSYLRMATVEFPVVVAKRFPTARYSLVEAKPVTGRWHQLR
                     RHFNRISHPLIGDADHGDCRQNRFFRNELGVPGLCLKAQSLELTHPWTQEPLKIEASS
                     CEKWEKIYDLFRKNQ"
     gene            complement(8961..9620)
                     /locus_tag="VIG33_09090"
     CDS             complement(8961..9620)
                     /locus_tag="VIG33_09090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8271031.1"
                     /translation="MVNCLLCESSKTAYFTVKNKPARGYFHCEYCDLIFMNPSERLHP
                     QEEKARYDLHQNQDSSEYRQFLQPLVQEIQNHGRSMAIPPETLKILDYGCGPTAFLSA
                     LLAENSFKAVNYDLFYFPDTFSLQATYDVITCTEVWEHLHHPYDEITRLHQLLKPSGL
                     LAVMTASHPGVELFHHWHYRRDLTHVIFFSDKTMRWIGAHFHLELCKAQTPYWFFRKR
                     S"
     gene            9768..10775
                     /gene="gap"
                     /locus_tag="VIG33_09095"
     CDS             9768..10775
                     /gene="gap"
                     /locus_tag="VIG33_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY8271032.1"
                     /translation="MSKLRVGINGFGRIGRVLFRAGFEKLDIVGINSLDTIAGDAHLL
                     KYDSTHGIFDAEVSTDEHSLIVNGKRIPVSKTKSPAEIPWKEWGVDVVLECTGAFKEK
                     ADFIKHLAGGAKRVLVSGPAEKGADLTMVYGINHATYDPAKHTVVSNASCTTNCLAPL
                     AKVLNDSFGIEHGTMMTIHSYTNDQKILDAPHNDLRRARAAALSMIPTTTGAAKNVGL
                     VLPELKGKIDGISVRVPTANVSLVDFTFQAKTDVTKEVVNNALIEASKGALKGILAVE
                     TKELVSVDFNGNKHSSIVDLASTMIVGPRMVKVLSWYDNEVGFSNRMVDVALYMASKG
                     L"
     gene            10778..12001
                     /locus_tag="VIG33_09100"
     CDS             10778..12001
                     /locus_tag="VIG33_09100"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY8271033.1"
                     /translation="MASKGLKGIKTVRELEVAGKVVFLRLDLNVPLEDGKISDETRIK
                     ASIPTIQYLMEKGAKIVMCSHLGRPKHAEDRQFSLEPVAKRLTEILSLQGPAEVILWE
                     DVASDGVKALLQNMKKNQLVLLENVRFAEGETKDSEQLAQVWASYSDVYVNDAFGASH
                     RAHASIHALPILMKEKAIGFLIEKEVTVLDGLLENPKRPYLAVLGGAKVSDKIAVIEK
                     FLDIVDGFVIGGAMAYTFLKAQGHAVGKSLVETDKLNYARQMIERIEARNKSLLLPVD
                     HVATTSISDTASARVTADVNIGDNELGVDIGPKTMRAYTIALSEAATVFWNGPMGVFE
                     TPEFSKGTFAIAKALAESKGEKIVGGGDSAAAAEASGYADKMTHISTGGGASLEYLQG
                     DKLPGLEILRAKIRS"
     gene            12003..12758
                     /gene="tpiA"
                     /locus_tag="VIG33_09105"
     CDS             12003..12758
                     /gene="tpiA"
                     /locus_tag="VIG33_09105"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY8271034.1"
                     /translation="MKIFAANWKLFKSPKETREFFSLWGEAAQASLGGSKNEVVFFPS
                     AISLEAAGSAIEGLKTKYKLSYNLRFGVQNAYFQAQGAFTGENSAAVVKELGGKYVLI
                     GHSERRQLFAEKNELLADKVSFTQGLGLIPMLCIGETLQERDSEKTYDVLKAQLSEGL
                     SKADQAKSLVIAYEPVWAIGTGKVATPEQVQETHKDVFEILKVLGFTAPILYGGSVKP
                     DNAAGLIQLPYVDGFLVGGASLEVASFQKICSV"
     gene            complement(12762..14882)
                     /locus_tag="VIG33_09110"
     CDS             complement(12762..14882)
                     /locus_tag="VIG33_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271035.1"
                     /translation="MLRESLPQKFEAFLQGSFAKISPGEVYQWLKKTDVSKTVSQKTI
                     VDNLIKYVGSLGASQMKPEILAALVNQIDKDFKMSETTPDLFAYFKNELAKAVLLQPH
                     AIKTVFPSGANMTVDTKAAQGFALEVRGLSTVVALTRIQKAEDQIHMIEYIEGHRSDM
                     PEFARKVINEGNRGLPMTEIVKNVRERLLHESTIARTLIANTFLAGPSSMIYKTEGKE
                     KLYDHILKSVSSKNRSIANEIANALVRSLKESQSLAFAYVFGQKPEDNQDNKLSEARI
                     LKSLFEARGVAGVKLGQYLAFTGEMKEYRDVLAELQDSAMPLTYFEATQLLDKHYNGQ
                     WPLYYKVKNVLGTGSVNIAILIEDTRSGEELVVSVLRENVDVSIEEDFRSLKDFVTEL
                     TQTPEGKKKYGYLKGLLGIIKKSVSLELNKKNSFDRQTENRKLYDRTIRVHGESWQLS
                     TPAAVGVDKSSFFMGKASGITARKLFMENPKLYNEIMTQINGIELDILLGIDETGKPK
                     PISLMANPDFHDGQLLIDVKTKKVFILDFGQAVPLTNRDRELAIDLLRAIEMLRMHES
                     VPLAAKLGFKKISLSILNQLSREYSNSKQDLLRVENLEILGGKTERMDIFVQVVSLLS
                     QKQVELPISVIHWILGLNRQIVLANKIGKPIENQIRNLLLTRTFHLDLKTYNRARLLN
                     RQLRGFIEHPEKTIMSCKEIFSST"
     gene            complement(14882..>15117)
                     /locus_tag="VIG33_09115"
     CDS             complement(14882..>15117)
                     /locus_tag="VIG33_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271036.1"
                     /translation="LELIEKGLTDLHSEFKRAHENPVAIAMKMNRLKNKMRSSASASL
                     FLFDVLAKNLRRFKASSNGIHIIVPSPTSIQML"
BASE COUNT         4156 a         3063 c         3596 g         4302 t
ORIGIN      
        1 acccagcagt gagcggtggg ttaaatattt taaaatttga ttatgacgga attcatcagg
       61 aacttcttca agtggaaagc tcccctttag cccaacattt tttgttggtt tacacaggaa
      121 ggactcatca ttcgggtatg aataattttg aagtgatgaa gggagccgtg gctaaagatc
      181 caacaacact taaagctttg aaagatatca aagatattgc ggaacgtatg gaaaaagtag
      241 tccgcgaatc ctgctggagt gagcttcccg gactttttcg tgaggaattc acagcgcgtg
      301 tgcggttggc accggcattt acgagccctg aaattgaaaa gttaaatgag ctttctttgc
      361 agaacggagc tcatgctgtt aaaatttgtg gagctggggg cggaggctgt gtgctgatct
      421 ggactccaga agaaaaaaag gaaggagtgg ccaaagcatg tcaaaaagca ggattccaag
      481 ttctcgccac tcgtcccgtg aatccactcc tgtaggggat gtcatccgct ttgtcggttt
      541 atctctcggt ggaggcaaag cagataaagc gtgtatggct attttggaat attatccaaa
      601 gcaccataag gttttcctcg caaaaatttt cgatagaatt aaaacagaag aggctatttc
      661 tgcggatcta aagattcacg aaattctcga ccaataccac ggcatgatac atagtgtggc
      721 gctggatacc ccgtggagtt tgccgacatg tcttcgttgt aaattaaaat gccccggcta
      781 cgaaaattgt caagagcctc atattcagtg gatgtgggac tatgtaaaag aaaaaaatgc
      841 caagaaaaaa ccaaagaagc ttttcacacc ttatactcaa agggccgtag agatgcatct
      901 ggcgacagga ttcgatgagc ctttccaaat tccgaatgct atgggagcga attccgctcc
      961 tcttttagca agggctcatt ttattttgcg ccgtcttgat attcaaaccc tcgaggtttt
     1021 tcccaagttg tctatctgga ggatcggttt atccctacat attctaaaat caaatttacg
     1081 ttcccataaa cattcggcag tcggagaaga cagtcgtaaa ggaatcctca aggagctggg
     1141 cgagcacaac attgccttcg tttatgatca agatcggcgt ttaatgattg aaaacaatca
     1201 tgcctttgaa gctttcattt gcgctatgac tgcttttttg gagtacaaag gtcagaccga
     1261 agctcgtcct cgaggatttc caaaaaatga agattggatt tctttcccaa aagagaagat
     1321 taattggaaa ttttagactc aaattataaa ctcaatttta atctctaaga tacttcgatt
     1381 gggacaggct tgaagttcat ataaaaagcc tccatttggg ttgcaaatgg aggctagctt
     1441 taatcatcat tcaatatgga aaattactta agttgttcca tggcatatgt gagccaagca
     1501 cttaattttt tagaagtgat gttggagtag acaataaatc catcacaacc aagggttccg
     1561 cgacttgtaa cgccaaacaa gtggtattcg ccatttgctt gttttacata agcagggcca
     1621 ccagaatcac cttcgcaagc ggcttgccca tgctgttggt caaaaacaat ctcagtatcg
     1681 ttgaagtcac cagcaacttg aagttcagtt gttcttaagt aaccttctcc actcgcctct
     1741 tctttatagc attttccaat ttctccagcc ttagaggttt cacaaaaaat ctcaccttta
     1801 agtacttttt cttgaaagtc agggatttcg tctttattaa cttcggttag aacatctttg
     1861 ctcacaccgt aaccagccac ggtaattgtt aggccctcgg ttaatacctc tgcagaattc
     1921 aaaagtgtag ctggcttgaa ccccgctgga acaggacctg caaatttgat aagagctgtg
     1981 tcacctgttg cagcattagg gacttgagaa gtcttacccc aatttggatt caccacagtt
     2041 tttacgccac gacgaagttt ttgcagaata agatttttgt cttggctttg gaaaacgcca
     2101 ggcaaatccg tagagaacac gatcaagaga tcttcagccg cagcgccgat acaatgtgcc
     2161 gcagttaaaa cgatattatt ttccagtaaa gagccagtgc aaagagctcc ttttttcata
     2221 tcgtaaattc caacgatgct tttttgaaga gctgcacctt caggaacttc ggtaccccca
     2281 atgattgagg aagtttttga tgagctgttc atgttgtttt tattagctgg cgtacaacca
     2341 actgcaagtg tgacaaggac aatcgctgcg gaagcctgtg taaataattt catttaaaat
     2401 cccccccctt aaaaaatacc tcttcaaaaa caaactgcta aaaagttgcc aacttagacc
     2461 aaaagcgcgg aagaacggca atgtttattt ttgaatagag cccataagtt ttccttatag
     2521 gatctgtgcg caaagcttgg caaagcacaa ggctccagca ataatggttc ttatgtggcc
     2581 tgagatgagg agagcttaat ggcagagtgg gaaagtcgca gcgaaatcaa ttcagacgta
     2641 gttttcttcc cctatgttcc ggtaggactt gagaaaaata gcgaagaagt ttttctttgg
     2701 gatcttgata aaacatattt agacaccaca attgactcgc tgcgtggttt gttgcagacc
     2761 attttagaga gagcctggaa taaacgcaac attcccggga ccagcacgct gattcgggcg
     2821 ttgtccatct atcgcgctaa gaaaagtgga caaaagtatt ttcccattta ttttattaca
     2881 gcgtctcctc cacagatgga agagaggatt tcggagaaat tttccatcga tgaaattcat
     2941 cctttcggtt gtttttataa agataatttg aaaaatctcc gccctggccg tttttggcgc
     3001 ttaactaaac aaataggtta caaactccag gctctgatgc aactgaggac tcgcttgcat
     3061 cctcaggttc gtcaaatctg ttggggtgat gatagtgaat ctgacgcggt tatttacaat
     3121 ctttattccg atatctgtgc tcggagaatt ggacctcacg aaattcgtaa tattctggag
     3181 aagcttcatg tgactgggga gcaagtggat cagatcctcg tattgcagtc gcagattcct
     3241 gagaatgatc cggttgaaaa aatctatatc aacctcgcaa cagatacaga tcctgattat
     3301 tatttaaaat tcggacgtcg cactttggcc acttataata cttatcaggt ggctctagat
     3361 ttagtgcagg atcagcgtct gtcttttgag ggtttgtatg cggtcgctca agatatgatt
     3421 tataattatg gtttttctcc tgaggaactg atgcagagtt ttgatgaact cattcgtcgt
     3481 ggagttctgg gacagacttg ttatgaaaaa gttcgtcctt tccttgaaga gaagggtttg
     3541 attttccgtg attttgaacc ttcaatggcg cctttaaaag agaaacacgt cattgatggc
     3601 aaggtcttcg aaatggaagg agttcatgag ccctgggtcc atgagcgcgt ggattacttg
     3661 catgagagcc gttagtgcaa gaacacgcct gcgcaaagcg aagctttgaa ctttggattg
     3721 tagattttgg gaaagttatt gatttttaca atccaacgtg tgccaggccc aggggctgca
     3781 atgggtgcgt gcggtgagtg ctcggtaatt ccactactac ttaggagatc ttgtaaacga
     3841 aatccattaa agccccaata gtaggcatca accacccagt ccacttcctc atgttttacc
     3901 acagtaaatg catgatcctc gataatttga taaccatgat agttgctagc gcgataaatt
     3961 tcagcgtaag catgtttatt gtcgataccg gcggctttca aagcaaaatg taaaaccaga
     4021 gcgtgttctc tgcagacgcc agcgccgcat actccatatt cacttaaagg cacatccttt
     4081 tttgccagac gatatttctt gagaaggcgg cgatagtaag ggttgtggta gttatggtat
     4141 ttaaaaaaat cagtacgcat cagttcaacg atgaggccta ttttatccca gtacgaaata
     4201 ttttggaggc cgatttcgcg agccgctact aagagagatt ttaaaggttc gctttgcagg
     4261 tcaatctggt agtcgggttg tattccccta ttccttacag agccttccac ataggctcca
     4321 tcttggatat ccgtgaggcc tccagggaga acctcataat tttcgatatt ttctggaaga
     4381 gcgtgagtgg caaaggaaag aaggatcaca agtaaaaagg tccctatagg taggcctttt
     4441 aaggcactac agctgtataa aaaatgaaca gttctgggtt tatcatttac aggtgatttc
     4501 atataaatgg tttaaagcaa aataaatgcc attgaataat aaatacgacc aagtttttgt
     4561 ttgaatttac tcaaaactga ctgggaaagt gctggtgatc ggatcgcctc taaaggaatt
     4621 aaattcgatc cggcgcaggg cttcactgag gcagcgtttg aattcagcat ttgaaatcgt
     4681 tgagttggtg acaccggcgc tggcaatttt tccgcttctt tcgatagtga aagtcataac
     4741 cacttggccg accacaccgg gagttctctg taaaagttgt ttataacatt tgtcaaaggc
     4801 cgggatctga cgtttcaaag tttcttggat gtactcagag cttaagctat cagcggaagc
     4861 tggggtggga ggagaatcag cgacaacagg agctatttct ggaaaagtgt tttgtggatt
     4921 ttctttgcga aggatactct catattcact ggggctcagg cgttgaccat ttttggaaat
     4981 catcaatgca ctgccacgac cgaaactctc aatttgcaca tcgccacgtt taagaatgaa
     5041 aatcgctttt tctccttctt gatctaaagt gatgagggcg ttgtcaagaa cgcggagacg
     5101 ataacctgaa tttaattcaa agctcgcatc cccattgggg cccgtttcca cggtgtccaa
     5161 ataataaaga tttgcacttt ttttgagagt ttctttttca gttatatttt tgtgaagaac
     5221 atagacttca ccagagttat agataacgcg agccaagact ttgaggccgg aggattgatt
     5281 ctcaagccgg gccgacatga accaggaaag acccacgaaa aaaagaccag caataactaa
     5341 agaagggata agccacttgt tcttcgccat aagaaaagag tatagcgaag aactaaaaag
     5401 aacacaggat tattttgcag gagcttgcgt gggagctgaa gtcgttggag ccgcagccgg
     5461 agtggctggt gttgccgccg gaatggcgga agttgcaggg agtttatcca aaatgctctt
     5521 agattcacga gcagaaagaa ccgtcaaacc gatcgaagta agagcgaaga cgatagccgc
     5581 gatgcgagtc attttggcag ccaaagtgct ggccccagtt gctccaagaa ccgagttaga
     5641 tccgccgccg ctacccatac caagagcgcc gccgcctttt gagtcttgga tcaaaaccaa
     5701 gctaataaga accagtgcta caataacatg caaaacagcg atgaatgtac ccatactttt
     5761 acctatctcc taaaggtggc cactttacag aaagcttgta tagaaagtcc actgaaaatg
     5821 gggaggaagc cacatgacac taagcgctat tattaatttt gacactcaga tagggcgcct
     5881 gccataagac tcctatggca ttccttgtat ttgaaggtct tgacggttca ggaaaaagtt
     5941 ctttgatgcg agctttggaa gcagagcttt ccaagctggg catttcattt catcgcaccc
     6001 gcgaaccggg cgggactctt ttaggagatg aaattcgtaa tatgatttta cgcaaacagg
     6061 aaggaactac tccggttcca cgggcggaac ttctattgta cgaagccagt cgtgctcagc
     6121 atgtggatga gattattaaa ccgaagttgg ctcaaggaca ctgggtcttg agtgatcgct
     6181 ttgcggcaag ctccgtggcc tttcaagccg gtggccgtga agttcaagaa aaagatgtgg
     6241 tggtattaaa tcattttgcc acggatggac ttaaagcgga tctcacagtc ttacttgacc
     6301 ttagtgtcga ggattctcgc aaacgacaag tgcagcgcac ctctcaaacg ggggaagcgg
     6361 cggatcgcat tgagtctgaa aaagattctt ttcatgagcg cgtgcgcctg tcttttttaa
     6421 aacaagctga agatgacaga caaggctggt tggtcctgga tgcgcgctct tcgaccgagg
     6481 aaatgttaaa aattctttta aagaccctca gggagcgaaa gtggcttctt ccatgaaccg
     6541 tcttttagac caagtttttg gtcacaaaga agtgatttcc aagcttctcg cgacttttac
     6601 cggtgagaga ccaggacaga cctttttatt tgtgggacct catggggtgg gcaaaaaaaa
     6661 agtcgccctt ggttttgccc aagccttact ttgtgatact ttgcgcacgg cttgcggtgt
     6721 ttgtgggtct tgtcgccgag tcgccgcagg aacccatgaa tctctcaaaa taatcgcttc
     6781 tgaaggaccg caaataaaaa ttgaagaaag tcgtgaagtg attcaattcc tcaaccttca
     6841 agggattact cagcgtcgtg tcgtggtcat tgaccaagtt cagactctga atttgcaggc
     6901 ggccaattct ctcttgaaaa ctctggaaga gccaccgccc gaaacttatt tctttttaat
     6961 cgctcccagc gtggcagggg ttctttcaac cattcgttcg cggtctcgag tggtgagctt
     7021 tcatcctctc gcggcggaag agatcgcgcg aggagcgggc tcagcaccga cctggaccat
     7081 tcgggccagc caaggcagct ttgagaaatt agcccggtta caagaaggac ctgagcaaga
     7141 gcttcgcagg gaggcactct cattgttgca acaatttctc gaagacaaag agtttttaac
     7201 ggccgataac tggcgtgaca gttttaaaga aagagtgaag tcgcaaagaa tactttctta
     7261 ctggatttct tttttacgtg atgttttgta ttttcaagag gatcagaaga attcgatcat
     7321 gaacgtggat caagtggatt ttttaaagag catgtctgag cgaagtcgaa atcagatttt
     7381 agagcttatt gagaaggcgt tgacagttga gaaagagatt tttcagaacc gcgatgtgca
     7441 gctattgatg gaagaattct ggattttgca aaatcgcgga ggagaggtgt aagatgttgg
     7501 agtggatgga tttacatgct catctaaata tgttggaaga aggtccagaa gaggctctac
     7561 gcctcgctcg agaagcgggt gttgtaaagg tggccaccat tgggacggaa cctcaggatt
     7621 tgcctttagt cctcgagctt gctgaaaaaa attatcccaa tgtattttgt accttaggta
     7681 ttcatcccca tgaaggtcag atttataccg ctgaaattgg ccagttcata gaaaacaatg
     7741 tttccaaacc ttgggtgatt gcggtgggcg aaatcggcct tgattattat tatgaacatt
     7801 cctcccacga agagcagaaa caggcttttc gtgcccagct cgagattgcc gcgcgcaccc
     7861 agatgccggt gcaaattcac acccgcgatg cggatgaaga caccattgaa attatgaaag
     7921 aatttaaggg cggggtgaag ggcatcattc attgtttcac aggcactccg tggctggccg
     7981 agcaggcgct ggccttgggc tacaatattt ccattagcgg ggtggtgact tttaagagcg
     8041 ccgaggcttt gcgggcgatt attcgaacct tgccgctcga tcgtattcat gttgaaaccg
     8101 atgccccctt tttagctcct attcccatgc gcggacgcaa aaatactccc gcttttgtga
     8161 ctcatacggc gaaagtggtc gcggacctta agggtgtctc gcttgaagag ttggcggaaa
     8221 tcactcggaa aaatgccctg aaaatgtttc cgaaaattat ttggtaaaag tatttaatag
     8281 aggagaatga gcctgaaact tctttttcag gatgagcatt ttattgccgt cgataaacct
     8341 tcgggctttc atgttcaccc ccatgaagac aaaagaaatc gtatctctcg agataaagtc
     8401 tgcctttatc tggctcgtga tatgatgcga cggcatgttt atccggttca tcgcttggat
     8461 gcggggacga gcggggtttt actttttgct ctttcttccg cttcagcgag cgttctttgt
     8521 aaactctttg cggaaaaatc attgcaaaaa acctatcatg ccgtggttcg aggatacact
     8581 cctgaacagg ggaatattga attgccttta gagtcggatt ccaccgggga tctggttgaa
     8641 gcaaagactt cttatttaag aatggcgacg gtggagtttc cggtggtggt ggctaagaga
     8701 tttcccaccg cgcgatattc cctggttgaa gcaaagcccg tgactggtcg ctggcatcaa
     8761 ttgcgccgcc attttaatcg tatttcccat cctctgatcg gtgacgcgga tcatggagac
     8821 tgccggcaaa atcgtttttt cagaaatgag ctgggggttc cgggtctttg tttaaaagcg
     8881 cagagtctgg aactcacgca tccctggact caagagcctt tgaagattga agcgtcttct
     8941 tgtgaaaagt gggaaaaaat ttatgatctt ttccgaaaaa accagtaagg agtttgagct
     9001 ttacaaagct cgagatgaaa atgggcaccg atccatctca tggttttgtc ggaaaagaaa
     9061 atcacatgag tcagatcgcg gcgatagtgc cagtggtgaa aaagttcgac gccgggatga
     9121 cttgcagtca tgacggcgag taggccagag ggttttaaaa gctgatgaag acgcgtgatc
     9181 tcatcatagg gatgatggag atgctcccaa acctcagtgc aggtgatgac atcatacgta
     9241 gcttgcagtg agaatgtatc cggaaaataa aaaagatcat agttcactgc tttgaaagaa
     9301 ttctcagcca ggagcgcact caaaaaggcg gtgggtccgc agccataatc caaaatttta
     9361 agagtttccg gcgggatcgc catcgagcgg ccgtggtttt gaatctcttg caccagaggt
     9421 tgcaagaact ggcgatactc agaactgtct tggttttggt gaaggtcata gcgagccttc
     9481 tcttcttgag ggtgaagtct ttcggaggga ttcatgaaaa taagatcaca gtattcacaa
     9541 tgaaaataac cgcgagccgg tttgtttttc acagtaaaat acgctgtttt tgaggactca
     9601 caaagaaggc aattcaccat cttatagctc cagattttaa ggggtgtatc aatacaatct
     9661 tctcaacact gcggcgcact actaaaaagt aagccgtacc ttgatttctc ggggctggtg
     9721 ccgcagattg gtgggcatct aaatccaaat attctaggag tttatttatg tctaaattgc
     9781 gcgttggtat caatggtttc ggtcgcatcg gtcgggttct cttcagagcc ggttttgaaa
     9841 aactggatat cgttggaatc aactctttgg acactatcgc gggtgacgct catttgctta
     9901 agtacgactc tactcatggt atttttgatg ctgaggtttc taccgatgaa cacagcttga
     9961 ttgtgaatgg aaaaagaatt cctgtttcta aaacgaaaag tcctgccgag attccgtgga
    10021 aagaatgggg cgtggatgtg gttttggaat gcactggagc gtttaaagaa aaagcggatt
    10081 ttataaaaca tttagcaggt ggtgcaaaac gcgttttggt ttctggcccg gcagaaaaag
    10141 gggcggactt aacgatggtt tatggtatta accatgcgac ttatgatccc gcgaaacaca
    10201 cggtggtttc taatgcctct tgcaccacca actgcttggc tcctcttgca aaagttttga
    10261 atgacagttt cggtattgaa cacggaacca tgatgaccat tcactcttat acgaatgatc
    10321 agaaaatatt ggatgctccc cacaatgatc ttcgtcgtgc ccgtgccgct gccctgagta
    10381 tgattcccac tacaactggt gctgcgaaaa acgtgggatt ggtattacca gagttaaaag
    10441 gtaaaatcga tgggatctca gtccgcgtgc ctacggcaaa cgtaagtttg gtggatttca
    10501 ctttccaagc aaaaacagat gtcactaaag aagttgtcaa taatgctttg atcgaagctt
    10561 ctaaaggggc tttaaaaggc atcctggccg ttgagactaa agaattagtg agtgttgatt
    10621 tcaatggaaa taaacactct tcaatcgtgg atctcgcttc gacaatgatt gttggtcctc
    10681 gcatggtaaa agtcctttct tggtatgaca atgaagtggg tttcagcaat cgtatggtgg
    10741 atgtggccct ctacatggct tccaaaggac tttaattatg gcttccaaag gacttaaagg
    10801 tatcaagaca gttcgtgagc tggaagtggc cggaaaagtt gtattcttac gcttggatct
    10861 caatgttccg ttggaagacg gaaaaatctc tgatgagaca cggattaagg ccagcattcc
    10921 gacaattcaa tatctcatgg aaaagggcgc aaagattgtg atgtgctctc atttaggtcg
    10981 tccgaagcat gcggaagatc gccagttttc attagagcct gtggctaaac gcttgaccga
    11041 gatcctcagc ttgcaaggac ctgctgaagt gatcttgtgg gaagatgttg ccagtgacgg
    11101 cgttaaggcg cttttacaaa acatgaagaa aaaccaattg gtattgctgg agaacgtgcg
    11161 ctttgccgaa ggtgagacca aggacagcga acagcttgct caagtttggg caagctattc
    11221 agatgtctat gtgaatgatg cgtttggtgc ttctcatcga gctcatgcga gtattcatgc
    11281 tcttcccatc ttgatgaaag aaaaagccat tggttttctg atagaaaagg aagtgactgt
    11341 attggacgga cttctagaaa atccaaaacg cccctatttg gcggtgcttg gtggagcgaa
    11401 agtttcagat aagattgcag tgattgaaaa atttctggat atcgtggacg gcttcgttat
    11461 cggtggagcg atggcttaca ctttcttgaa agctcagggt catgctgttg gtaaatcttt
    11521 ggtagagact gataaattga actatgcaag gcaaatgatc gagcgtattg aagctcgcaa
    11581 taagagttta ttattgccgg tggatcatgt ggcgacgact tccatcagtg atactgcttc
    11641 ggctcgagtg actgcggatg taaatatcgg tgataacgaa ctcggcgtgg atatcggtcc
    11701 aaaaacgatg cgtgcttaca ccatcgcctt gagtgaagcg gcgacggtat tttggaatgg
    11761 tccgatggga gttttcgaaa ctccggagtt ttctaaagga acttttgcca ttgccaaagc
    11821 cttagctgaa agcaaaggtg agaaaattgt cggtggtggt gattctgcgg cggcggcaga
    11881 ggcttcaggc tatgctgaca agatgactca catttctacc ggtggcgggg caagccttga
    11941 atatcttcaa ggggacaaat tgcccggtct agaaatcctt cgtgcaaaaa tcaggagcta
    12001 ggatgaaaat ttttgctgct aactggaagc tttttaagtc gccaaaggaa actcgtgagt
    12061 tcttttctct atggggagaa gctgctcagg cttctttagg tggcagcaaa aatgaagttg
    12121 tcttttttcc ttcagccatt tcattggaag cagctggctc tgcgattgaa ggtttaaaaa
    12181 cgaaatataa actttcctac aatcttcgct ttggcgtgca gaatgcttac ttccaagctc
    12241 aaggtgcctt tacgggagaa aattcagcgg cggtggtgaa agaattgggt ggtaagtacg
    12301 ttttgattgg tcacagcgaa cgtcgccaat tgtttgcaga gaaaaatgag ttattagcag
    12361 acaaagtcag cttcacccag ggtctggggt taatccctat gttgtgtata ggagagactt
    12421 tacaagaacg cgacagcgaa aagacttatg atgttctgaa agcccagttg tcagaagggc
    12481 tttcaaaggc agatcaagca aaatctttag tcatcgccta tgagccagtt tgggctattg
    12541 gcactggtaa agtggctact cccgaacaag ttcaagagac ccataaagac gtttttgaaa
    12601 ttttaaaagt ccttggtttc acagctccga ttttgtacgg tggaagtgtt aagcctgaca
    12661 atgcggcggg cttgattcaa ttgccttatg tggatggctt cctagtgggt ggagcttcgt
    12721 tggaagtcgc gtcttttcaa aaaatttgca gcgtttagga attaagtact agaaaaaatt
    12781 tctttacaag acatgatagt tttctccgga tgttctataa aaccacgcaa ctgtctgtta
    12841 agaagtcgag ctctattgta ggttttcaga tcgagatgga aggtcctcgt tagcaatagg
    12901 ttgcgtattt gattttcaat aggttttcca attttattag ctagcacgat ttggcggtta
    12961 agtcctaaga tccaatggat gacggagata ggaagttcca cttgcttttg tgaaagcagg
    13021 gaaactactt gtacaaaaat atccattctt tcggtttttc ctccaaggat ctctagattt
    13081 tccactctta aaagatcttg tttggaattt gaatactcac gggagagttg attcagaata
    13141 gataaggata ttttcttgaa tcctaacttt gcagccaatg gaacgctctc atgcatgcgt
    13201 aacatttcta tagcccgtaa taaatcgata gcaagctccc gatctctgtt tgtgagggga
    13261 accgcttgtc caaaatctaa tataaaaact ttcttcgttt ttacatcaat taaaagctgt
    13321 ccatcatgaa aatcgggatt ggccatcaga gaaatgggtt taggctttcc tgtctcatct
    13381 attcccaata agatatctaa ttcgatgcca ttgatctgag tcattatctc attatacagc
    13441 tttggatttt ccataaataa ctttcttgct gtaatgccac tggcttttcc cataaagaaa
    13501 gatgatttat caacccctac ggctgctggc gtcgataact gccaactttc tccgtgaaca
    13561 cggatggttc gatcataaag ctttctgttt tctgtctggc ggtcgaaaga atttttcttg
    13621 ttgagttcta gactgacgga ttttttaata attcccagaa gcccttttaa gtagccgtat
    13681 tttttcttac cttcgggagt ttgggtcagt tctgttacga agtcttttag acttctaaag
    13741 tcttcctcaa tggatacatc gacgttttca cgcagcacgc tcacgacaag ttcttctccg
    13801 ctgcgggtat cttcaattaa aatagcgata ttgactgagc cagtccctaa aacatttttc
    13861 accttatagt agagaggcca ttgtccgtta tagtgtttat ccaaaagctg ggtggcctca
    13921 aaataagtga gaggcattgc tgaatcttgg agttcggcga ggacatccct gtattctttc
    13981 atttccccag tgaaagccaa atattgaccc aattttactc cggcgacgcc gcgggcttca
    14041 aagagacttt ttaagatccg cgcttcgctt aatttattgt cttgattgtc ctcgggcttt
    14101 tggccaaaga cataagcaaa tgcgagagat tgcgactcct ttaaagagcg aactaaagca
    14161 tttgcgattt cattggctat agatctattt ttagagctta cagattttaa aatatgatca
    14221 tataattttt ctttgccttc ggttttataa atcatggatg agggaccagc taaaaatgta
    14281 ttggcaatga gagtgcgagc gattgtgctt tcatgtaaaa gtctttccct tacattttta
    14341 acaatctctg tcataggaag cccacgattt ccttcattaa taacttttcg ggcgaattcg
    14401 ggcatgtcac ttcgatggcc ctcgatatat tcaatcatat gaatttgatc ttcggctttt
    14461 tgaatacggg ttaaagcaac gacggtacta agtccccgca cttcaagggc aaatccctgg
    14521 gcggctttgg tatccaccgt catattggcg ccggaaggaa atactgtttt aatagcatgg
    14581 ggttggagta aaaccgcttt ggccagttca tttttaaaat aagcaaagag atccggcgtg
    14641 gtttcactca ttttaaaatc cttgtctatt tggttcacaa gagcggccaa aatctcaggt
    14701 ttcatttggc ttgcgccgag ggagccaaca tatttaatca aattatcgac aatggttttt
    14761 tgtgaaactg tttttgaaac atcagtcttt ttgagccatt gataaacttc accagggctt
    14821 atttttgcga atgaaccttg cagaaacgct tcgaattttt gagggagtga ttctcggagc
    14881 atcagagcat ctgtattgag gttggcgatg gcactataat gtgaatacca ttgctcgagg
    14941 ctttgaatct gcggaggttc ttggcgagga catcaaatag aaataaactt gcggaagcac
    15001 tggatctcat tttgttttta agacgattca ttttcatggc gatcgcaact gggttttcat
    15061 gggcacgttt aaattcggag tgaaggtctg taagaccttt ttcaatcaat tccaagt
//
LOCUS       DATBTE010000248         2399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2287765, whole genome shotgun sequence.
ACCESSION   DATBTE010000248 DATBTE010000000
VERSION     DATBTE010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2287765"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VIG33_09120"
     CDS             complement(<1..998)
                     /locus_tag="VIG33_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY8271037.1"
                     /translation="MSKKFQLKNGLKVLFLESHKSPVVSVQMWVKTGSADEKKTEEGI
                     SHFIEHLVFKGTRKYKVGEIASLVEASGGELNAYTSFDQTVFYVTISKQFADVGLDVI
                     SEMMGFPTFDAEEINNEREVVIEEIKRGQDSLSRAGSQMMFSSVFKGHPYAVPVIGYE
                     KNVRKFSAKKIADFFHSRYVPRNMFLVISGDFESVQMKQKVQKYFGEFQDYKLRKSTR
                     PKVKLPKKNQIKVEKSTFEQTVAYLAWPAPNVKHKDVPALEVLSLILGQGDSSRLMHS
                     LRLETPLTNSVGSYVFTPQDAGVLAISMGLNIENLQESLDKIREHIVEIIRTPPAAG"
     gene            1017..2192
                     /gene="hemW"
                     /locus_tag="VIG33_09125"
     CDS             1017..2192
                     /gene="hemW"
                     /locus_tag="VIG33_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY8271038.1"
                     /translation="MSFGIYVHIPYCIQRCTYCDFATYEQFKILPPEKYFELLFEEIR
                     QKKGYYPARTIDTLYFGGGTPSLVPSELIVSLIQELAKYGFTTGPQTEITIEINPATV
                     DEKKLATYLSVGVNRFSVGAQTFDDRLLKMVHREHNARQTLDTLALLRRYNINFSFDI
                     LFALPSQSVEGLRRDIDIAIEQGAKHLSPYCLTVPEGHPLSKARPIEDDQVAMFEIIA
                     EKLQSRGFHQYEISNFSLPGYESRHNILYWTDEEYWGLGLSAHSYSKSTHWGLRYWNL
                     SNINEYEKQILASAGQRFYNPIDHLPKEQFEKLVKHQSLTDFCHTSLRMLEGLSPEKL
                     ALKFSKETAEEVLKILRRLQASQWLEINDKGHWSMTKEGILISNQIFTQLTFLEGEI"
     gene            2283..>2399
                     /locus_tag="VIG33_09130"
     CDS             2283..>2399
                     /locus_tag="VIG33_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY8271039.1"
                     /translation="MDQNGNSSEETSFDVSSEIKKLQEQVEKYKNDYLYLRAE"
BASE COUNT          693 a          550 c          456 g          700 t
ORIGIN      
        1 cctgccgcag gaggagtcct aataatctcg acaatatgct ctctaatctt atctaaagac
       61 tcctgaagat tttcgatatt cagacccata gaaatggcta aaacccccgc atcttggggg
      121 gtaaatacat aggagccaac tgaattcgtc agtggcgttt ccaaacgcaa agaatgcatt
      181 aaacgggaac tgtccccctg tcctaagatc aaactcagca cttccaacgc tgggacatct
      241 ttgtgcttca cgttgggagc aggccacgcc aaataagcaa cagtttgttc aaaagtggat
      301 ttttccactt taatttgatt ttttttaggc agttttactt taggacgtgt cgatttacgc
      361 aatttgtagt cctgaaactc gccaaaatac ttttgcactt tctgtttcat ctgaactgac
      421 tcgaagtctc cggaaatgac caggaacatg ttacgaggca catagcgaga atgaaagaaa
      481 tccgcaattt ttttcgctga aaacttacga acatttttct catatccaat aactggaacc
      541 gcataagggt gtcccttaaa gacgctggaa aacatcattt ggcttcctgc acgactaagg
      601 ctgtcttgac cccgttttat ctcttcaatg actacttcgc gctcattgtt aatttcttcg
      661 gcgtcaaaag ttggaaagcc catcatttca cttataacat ccagacctac atcagcaaat
      721 tgcttcgaaa tcgttacata aaaaaccgtt tgatcaaagg aagtataggc attcaattct
      781 ccaccggaag cctccaccaa tgaggcaatt tcccctactt tgtatttacg agttccttta
      841 aaaaccaagt gctcgataaa gtgactaatg ccctcctctg tctttttttc atcagcactg
      901 cccgttttga cccacatttg tactgaaacg acgggagatt tgtgagattc taggaaaagt
      961 actttgagac cgttttttag ttgaaatttt ttagacataa tctaagtagt gctcctatga
     1021 gttttggaat ttacgttcac attccctact gtatccagcg ttgcacatat tgcgattttg
     1081 cgacctatga gcagttcaaa attcttccac ctgaaaaata ctttgaactt ctttttgaag
     1141 aaattcgtca aaagaaaggc tactatcccg cgcgtaccat tgacactctc tattttggcg
     1201 gcggcacccc aagcctggtt ccttcagaac ttattgtatc tcttattcaa gaactcgcaa
     1261 agtacggctt tactactgga ccccagaccg aaatcaccat cgaaatcaat ccagcaaccg
     1321 tagatgagaa aaagcttgct acgtatctta gcgtcggcgt caatcgattc agcgtggggg
     1381 cacagacttt tgatgatcgt cttttaaaga tggttcatcg cgaacacaat gcccggcaga
     1441 ctttagacac cttggcactt ttacgccgct ataatattaa tttcagcttt gatattcttt
     1501 ttgctcttcc ttcacaaagc gtcgagggac tccggcgcga tatagatatc gctattgagc
     1561 aaggtgccaa gcacctgagt ccttattgcc ttacagttcc cgagggccac cccctttcaa
     1621 aagctcgacc cattgaagat gaccaagtcg caatgtttga aatcattgca gaaaagttgc
     1681 agtctcgcgg ttttcaccaa tatgaaatct cgaattttag ccttcctggt tatgaatctc
     1741 gacacaatat tttgtattgg acggatgaag agtactgggg cctgggcctc agtgctcatt
     1801 cgtattccaa atcgactcat tgggggctcc gctattggaa cctgtcaaat attaatgaat
     1861 acgaaaaaca aattttagcc tctgcaggtc aacgttttta taacccaatc gatcacttgc
     1921 caaaagagca gtttgaaaag ctcgtcaaac atcagtcgtt gactgacttt tgccatacct
     1981 cacttcgaat gctggaaggc ttaagtccag aaaaattggc cctcaagttt agcaaagaaa
     2041 ccgcagaaga agtccttaaa attttgcgcc gtctccaagc ttctcaatgg ctcgaaatta
     2101 atgacaaagg gcattggtcc atgaccaaag agggcatctt aatcagtaac caaatcttca
     2161 ctcaattgac ctttttagag ggcgagattt aagtctgtcg cattgacttt tcttaatacg
     2221 tacacaatct tagcttggta tatatgcatt ataaggagac tcttgtgtct gatcaaaacc
     2281 cagtggatca aaatggtaac tcttctgaag agacgtcatt tgacgtatca tccgaaatta
     2341 aaaaacttca agaacaggtg gaaaaatata aaaatgatta tctctatttg cgtgcggaa
//
LOCUS       DATBTE010000249         2414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1058307, whole genome shotgun sequence.
ACCESSION   DATBTE010000249 DATBTE010000000
VERSION     DATBTE010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058307"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VIG33_09135"
     CDS             complement(<1..887)
                     /locus_tag="VIG33_09135"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEY8271040.1"
                     /translation="MLYILLWERLIMSESSEKKTISFGTNHGMTLVGVIVSAGIGAII
                     MAGLISMISDVFKSQRSIKARDAGRELTSALRELLSDSAICTASFGGGEPKEEGFTRT
                     QIVDSSPQRNIKYETDTKYLNNLVTITEFKIKNYSATNGSVNPNVGTAELIIKMDKVG
                     AAVGGSQMTVNYILQTVLDDSGKLVQCSAVGNVQSGPVGSLLTMMTATCPSGYLEANG
                     AAVKRSEYVLLFEAIKTIYGTGDGSTTFNLPDLRGYFLRGWNHASGNDPDVDSRTNSG
                     GGEVGDNIGTKQTDQFASHT"
     gene            1178..>2414
                     /locus_tag="VIG33_09140"
     CDS             1178..>2414
                     /locus_tag="VIG33_09140"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8271041.1"
                     /translation="MKNKEKIENLYIPNYEIIESLGESPLAFVYKGVHKQQPDQILVI
                     KLFKAGILSQQRRRYFLQKIEHLKILTDSRVAPPLRFEEYGQQKLLIRDYFSGIPINE
                     WIQKKASLSLEKFFTIALKLADIIDSIQKSGIIHGGIKPHNVIIEPKTLDVKIVDFIT
                     PLDIRNISHFIYDKDFIEDTLAYTSPEQTGRINHRVDFTTDIYSLGIIFYEILTRRLP
                     FFSTDPLELIHSHIAEEAPTADSFDLSVPAVVGKIISKLMLKQPEKRYQSGSGLYADL
                     YHCQQQWMQHKKIADFALGTKDRTHRVTFVSKMVGRDEEAQLILSEYTSVTQKEFKSV
                     FISGFSGIGKTRLIQELQRPIVKNQGYFTSGKFDVYQKNIPYSSLIQAFRNLTRTFLT
                     ENDERVAKWKNKILQAVGTN"
BASE COUNT          718 a          530 c          454 g          712 t
ORIGIN      
        1 gtatgactgg cgaactgatc tgtttgtttt gttccaatat tatccccaac ttctccgcct
       61 ccactattcg tccttgaatc cacatcaggg tcgtttccgg atgcatgatt ccatcctcgc
      121 aaaaagtagc ctcgtaaatc aggcaaatta aatgttgttg aaccgtcacc tgttccgtaa
      181 atagttttta ttgcttcaaa aagtaaaaca tattcagacc tttttacggc cgcgccattt
      241 gcttcaagat aacctgatgg acaagtcgcc gtcatcatgg ttaacagtga tccaactggt
      301 cccgattgca cgttacctac ggcggagcat tgaaccagct ttccagaatc gtctaaaact
      361 gtctgaagaa tgtagttcac tgtcatttgg ctcccgccga cagcagcccc aactttatcc
      421 atttttataa taagctctgc ggttccaaca ttaggattaa cggatccatt ggtagcagaa
      481 taatttttaa ttttaaattc agttatcgtc actaaattat ttaaatactt cgtgtctgtt
      541 tcatatttta tatttcgctg gggagaggaa tctacaattt gggttctggt aaacccctct
      601 tcttttggct caccacctcc gaagcttgcc gtacaaattg ctgaatctga taaaagttcg
      661 cggagagcag aggtgagctc gcgaccggca tctcgggctt taattgagcg ttgcgattta
      721 aaaacatcag aaatcattga aatcagtccg gccataatga tcgcaccaat cccagccgaa
      781 acaataacac ccacgagagt cattccatgg ttagttccaa atgagatggt ttttttttca
      841 gatgattccg acataattaa tctctcccag agtaaaatat ataacaattc agtctatcca
      901 tcggtaagtt aatgaagttt ctatatataa acaattagaa agttattgga cgcaattgtc
      961 tcagcgtaaa cgccgtagtt tggttgcgcg ttttcgagat tgatgtctcc tggtgagaca
     1021 gtttgaattt ttaaccggat ctgctctgac agatgaataa tgtgacttaa gacttaagtc
     1081 attattttgt ggaaaagccc ctgtttcttc tccgcaccct acaaaatctt ccgaatatcg
     1141 gtaagtgaaa ccgtttaaat tccgataaca attcatcatg aaaaataaag agaaaattga
     1201 aaatttatac attcctaatt atgaaattat tgagagcctc ggagagagcc ccttggcttt
     1261 cgtgtataaa ggggttcaca aacagcaacc tgatcaaata ctggtcatta agctgtttaa
     1321 agccggtatt ttgtctcaac agagacggcg ttactttctg caaaaaattg aacatctcaa
     1381 aatattgacg gactctcggg tggcgccccc tcttcgtttt gaagaatacg gacagcaaaa
     1441 actcttgatt cgtgactatt tttcgggaat cccgatcaat gagtggattc agaaaaaagc
     1501 ttccttaagc ttggaaaaat ttttcactat cgccttaaag ttggctgaca ttatcgattc
     1561 tattcaaaaa tcaggcatca tccatggcgg tatcaaacct cataatgtca tcatcgaacc
     1621 caaaacactg gatgtgaaaa tcgtcgattt catcacaccg ttagacatcc gtaatattag
     1681 ccacttcatt tatgataagg attttatcga agacaccctt gcttacacgt cgcctgaaca
     1741 aacagggcgc atcaatcatc gcgtggattt tacaacagat atctattccc tcggaatcat
     1801 cttctacgaa attttaaccc gtagacttcc ttttttctcg acggaccctc tggagttgat
     1861 tcactcgcat attgccgagg aagctcccac agctgatagt tttgacctct ccgttccagc
     1921 cgtcgtagga aaaataatct caaagctcat gctcaagcaa cctgaaaaac gttatcagtc
     1981 cggaagcggg ctttatgcgg atttatacca ttgccagcaa caatggatgc agcataaaaa
     2041 aattgctgat ttcgccttgg ggaccaagga tcgcactcat cgagtcacct ttgtttctaa
     2101 aatggtggga cgtgatgagg aagctcagtt aattttaagt gaatacacca gtgtcaccca
     2161 aaaagaattt aaaagtgtct ttatctcggg attttctgga atcggtaaaa cgcggctgat
     2221 ccaagaactg caaagaccca tcgtgaaaaa tcaaggttat ttcacttccg gtaagttcga
     2281 cgtttatcaa aaaaatattc cttatagttc cctcattcaa gcttttagga atttaacccg
     2341 cacctttctc acagaaaatg atgaaagagt cgctaaatgg aaaaataaaa ttctgcaagc
     2401 cgtgggaacc aatg
//
LOCUS       DATBTE010000250         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_785715, whole genome shotgun sequence.
ACCESSION   DATBTE010000250 DATBTE010000000
VERSION     DATBTE010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785715"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VIG33_09145"
     CDS             complement(<1..194)
                     /locus_tag="VIG33_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271042.1"
                     /translation="MKRLLDIDSLLDKAFRHFVLTSERSYIFNIRNSGGIENFYSLLE
                     VELKTYPFLKIQEYRTIKEGL"
     gene            complement(201..>2611)
                     /locus_tag="VIG33_09150"
     CDS             complement(201..>2611)
                     /locus_tag="VIG33_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HEY8271043.1"
                     /translation="LNQEEHLNSQVEALAAELATSETALLLQLWEQSLENKKVALHAA
                     ETSRAKLGALFKKASLSISVKANVSDIFSTIKARMSELAEEIKKTSESNALLAEQVTL
                     LKQQATPLRQDIEHLSTHQTLIDPGLYNIKTAALKELKLPKNSLMFIGELLSVKSPEL
                     RIAAESALEPIAKNLLVHPDYLDVFTPWLNKNNLAKSITVKRLTALDLKETAVLQPSV
                     ENSILTTLEIRSSQEHPFSGYMALWLNNSFDYQVVEVKEFKKGKSLLVTKEGLVKRDS
                     FTMRKLSKNLRYTLGWNPEERIAQLAAELAQLLEEINTKEVTVKTTASATDDQRILLN
                     TFSEYKTVQDLEFINVDHLHEQIKDLRQQITDLKRKDQKHHDRKELLEKLKLSLRKIQ
                     ETRAGMASELERLKRVLKELKERHSELRQTYESSRHLELLRVTESLEQIELRLNENRN
                     RLTKNQDEVLEHIEGKEKDLETKKDSLIAAVNRLLTQYQDRFGDPQLVYQLRDISILV
                     SVTQDWNLHKERLEGTELAGLEMKWQLFFSDILIKSIKDTLNEIKSQRLKIESNIQSI
                     NDVLKLNHFEKLPTENRFLQIHSESSSDARVRTFRRSCLDIENLLASPALRVKLENAS
                     KEVLAPLEKFVNDLKEDPKVREFVTDVRNHFEFEVRSWSQKDHSPEVELAETFTGSRN
                     DAKSSAQTTQLAYTLLASSLAYRFKFNDPVAGQNTPRMIILDEFGGKFDNEKPRDIIK
                     MLSQMGFQSLLVSPMTKAELLADSVNTINLVHKASASKSKVQHYVIQSKEDYDKLIRS
                     MAME"
BASE COUNT          680 a          532 c          559 g          840 t
ORIGIN      
        1 agtccctctt ttatcgttct atattcttga atttttaaga agggatatgt tttcagctcc
       61 acttctaaaa gagaataaaa attttcaatc cccccggaat tgcggatatt aaaaatgtaa
      121 gaccgctctg acgtaagaac aaaatgacga aaagccttat ccaggaggga gtcgatatcc
      181 agcaatcttt tcatattagg ctattccatg gccatcgacc gaataagctt atcataatcc
      241 tctttggact gaatgacata gtgttgaacc ttggatttgc tggctgaagc tttatggaca
      301 agattgatgg tgtttacact atctgctaaa agttccgctt tggtcatcgg gcttactaag
      361 agcgactgaa aacccatttg actcagcatt tttataatgt cccggggctt ttcattatcg
      421 aactttcccc cgaactcatc cagaatgatc attcggggag tgttttgacc agctactgga
      481 tcattaaatt taaatcgata ggctaaagat gaggccaata aagtataggc aagttgtgtg
      541 gtttgcgctg aacttttagc atcattgcgg gatccggtga aggtctccgc aagctccact
      601 tccggcgaat gatctttttg actccaagag cgcacttcaa attcaaagtg attgcgcaca
      661 tcagtcacga actcccgaac cttaggatct tctttaagat cgttcacgaa cttttccaag
      721 ggagccaaaa cttctttaga ggcattttca agctttaccc gtaaagccgg tgacgctaat
      781 aaattttcta tatcaagaca ggatctgcga aaagttcgga cccgcgcgtc cgaggagctt
      841 tctgagtgaa tttgcaaaaa tctattctca gtggggagtt tttcaaagtg attcagctta
      901 agcacatcat tgatagattg aatgttggat tcaattttca atctttgtga tttgatttca
      961 tttaacgtat cttttatgga tttaatcaga atatcactaa agaaaagctg ccatttcatt
     1021 tccagtcccg ctaattccgt gccttcaaga cgctctttat gcaagttcca atcttgagtt
     1081 actgaaacta gtatagaaat atcgcgtaat tgatagacta gttggggatc tccaaagcgg
     1141 tcctgatatt gggttaacaa ccgattgacc gctgcgatca aggaatcctt ttttgtttcc
     1201 agatcttttt ctttgccttc aatgtgttct aaaacttcgt cctgattttt cgtgagtctg
     1261 ttgcgatttt cgtttaaacg cagctctatc tgctcaaggc tttccgttac ccgtaaaagt
     1321 tcaaggtgac gagaactttc ataagtttgc cgcaattcag aatgtcgttc tttaagttct
     1381 ttgagaaccc tttttagccg ctccagttca gaagccattc ctgcccgagt ttcttggatt
     1441 tttcgaagac ttagttttaa tttttccaat agctctttgc gatcgtggtg tttttgatct
     1501 ttccttttta aatctgtaat ttgttggcga agatctttaa tttgttcatg aaggtggtcg
     1561 acattgataa attcaagatc ttggactgtc ttatattctg aaaaagtatt gagcaggatt
     1621 ctctggtcgt cggttgcaga ggccgtcgtt ttaacagtga cttcctttgt attgatttcc
     1681 tccagaagct gcgccagctc cgccgctaat tgagcaattc gttcttcggg attccacccc
     1741 aatgtatatc ttaagttttt agaaagcttg cgcatggtaa aagagtccct tttaaccagg
     1801 ccttcctttg tgaccagaag ggatttccct tttttaaact ctttgacctc aaccacctga
     1861 taatcaaaag aattattaag ccacaaggcc atatatccgg aaaatggatg ttcttgggag
     1921 gagcggattt ctaaggttgt taaaattgaa ttttcgacgg aaggctgaag aactgccgtt
     1981 tctttaagat ctagcgcagt aaggcgtttc actgtgatac tttttgctaa attattctta
     2041 ttcaaccaag gggtgaagac atccagataa tcaggatgca ccaaaagatt tttcgcgatg
     2101 ggttccaagg cggattccgc ggcgattcgt aattcagggc tttttacgga aagaagctca
     2161 cctatgaaca tgagactgtt tttgggcagt ttcaactcct taagggcggc tgtttttata
     2221 ttataaagtc ctggatcaat aagggtttga tgggtggaga gatgctcaat gtcttgccgc
     2281 aaaggagtcg cctgttgttt taaaagggtc acttgctcgg cgaggagggc gttgctttca
     2341 gaagtcttct taatttcctc ggccagctct gacatccggg cttttatagt ggaaaaaatg
     2401 tctgaaacgt tggccttaac agagatagag agtgaagctt ttttaaacaa ggctcctaac
     2461 ttggctcgtg aggtttcagc ggcatgaagg gcgacttttt tattttccaa gctttgttcc
     2521 caaagctgaa gtaaaagggc ggtctctgaa gtcgccaact cggcagccag agcctcgacc
     2581 tgcgaattca ggtgctcttc ttgattcagc g
//
LOCUS       DATBTE010000251        17585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1262774, whole genome shotgun sequence.
ACCESSION   DATBTE010000251 DATBTE010000000
VERSION     DATBTE010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 17585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17585
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262774"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..702
                     /locus_tag="VIG33_09155"
     CDS             148..702
                     /locus_tag="VIG33_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271044.1"
                     /translation="MIDSGTNPNAIKEELMDELDEELDEDLDEDEKVIDQFGDETLAA
                     DALEGQLALNPSSQLGEEEEDISLDEIDNILELKDDKYPKFTLAKNKARFLRMVSWYR
                     AKEEWIEVAPLSGVTKLFKQQTKELEGIRSSKLDYEMELETGTLTPSQRSYRRDELKM
                     CRVQEKMAVHLISKLQVKIKSGRR"
     gene            complement(756..1481)
                     /locus_tag="VIG33_09160"
     CDS             complement(756..1481)
                     /locus_tag="VIG33_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEY8271045.1"
                     /translation="MIKICSKKIMICGIIFGLTACGSNFDTPRLNDDPTEEQPADAAA
                     VQVEQVLVELPPVLSSQERESILAKYDYLDPNRVVPSKDLSDTVLYFEKNKSKIKNQN
                     YIAVINFSQSSKEKRFYIVDMKTGSVQAIHVAHGKGSDVDHDGYAERFSNQSGTNASS
                     IGFYTTAETYYGNHGLSLRLDGRSSTNSHARSRAIVLHGADYVREENIIQGRSWGCTA
                     VSMVLRNRIIAMLKGGALINAVK"
     gene            1779..3377
                     /locus_tag="VIG33_09165"
     CDS             1779..3377
                     /locus_tag="VIG33_09165"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEY8271046.1"
                     /translation="MERRLILLLLLVFLMTSCAQNGFQEPLASVQEAPAVTTYSPPIP
                     TKKIVKTREMVDGASQVSDFKLNIDGPKATSELKGTFSFQPADGNRIEVPVDVSGSIE
                     EYNSFMKPTNSDRLKVMDLQSGAKLTCLTKDCTESFVDIYVVHKGVIYHHQVEAKGAQ
                     PTTQQPATPVKESPAKDTFSANSKVKEQKVGDQKVADPKPKDQKATEPTKVPSEEATE
                     EATDKADDGSQYEHDIDEEAEAGTYVGEPQRDIEVLFPDLDANTGKADAGKDQESKKD
                     EKKDDKNIPPSKDINIQAKPPKTLPAQGSSASRLINVMVKGLGQAVTALGTRNLVKGH
                     LENPTDIFNYEESNPQVGFHILYPKRKAYFGTDDMLAMLSNMGRYSRNKVNGYVLNVG
                     DISAKNGGPLGKHSSHQMGLDADISYYFDAKEKQKGLVDAVKSSRPLRSFMAEEQWEM
                     FKTFVGQGNVDRIFIHPALKKELCGVAAKKGDLNSSNGTAFETLRVMRPEVHHDNHFH
                     LRLKCSTAQPRCRQMAPPAKGTGC"
     gene            3583..4026
                     /locus_tag="VIG33_09170"
     CDS             3583..4026
                     /locus_tag="VIG33_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8271047.1"
                     /translation="MSRTMMIMVSDNVEAIENAKKQWEHQDVTFQVYSPTQWREGMES
                     SFFRQQLMAGVPALAFGSSSVSGEVGGANILAFPAPSSTYNTTDIKIRKMDELEAHAI
                     ENAIVQYKGNLTEAAKALGIGRATLYRKVKQYHIDPSQARKKRAA"
     gene            4203..5273
                     /locus_tag="VIG33_09175"
     CDS             4203..5273
                     /locus_tag="VIG33_09175"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY8271048.1"
                     /translation="MKIWNKSCLARSELVRKNTPVADIIKDLNEQFSLLQTSHLLIYD
                     ADESKKVWSGESKETGIESQYVDGELVILKVHRNSPAELAGLRMGDVIYRINEELGTP
                     EMAEKISGGYLIQRHKKITEYKIQVKTIQREEEPEIGRISDRTVMLKVPSFRAEFFDR
                     DKWKRQVTELKKYQKIIIDLRGNNGGNFVAGLRLLSPFMCGPQDIGYLWKPKSKFKKD
                     AVFDDNLDDESQVNILDESFLVRLLTFADYDCLTASVAVMVDSGSASTAEMVAQALKD
                     YIGAKIFGSASAGQLLVGVWYPVPELAEGVRISIPEAVYQTRRGHKIEGPGVQVDKVL
                     YYHLEELQNGEDSWLKHAVQLF"
     gene            5515..6147
                     /locus_tag="VIG33_09180"
     CDS             5515..6147
                     /locus_tag="VIG33_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271049.1"
                     /translation="MSKSTVENKVLEFPKSQSLRKRLLDRGQDQKTVLSVSIISVLVI
                     TVFLNEWIVKSQHSVNSLTESRRGIASFEDRDSVEGIKWEHELAQKLGQEKDTSKSFS
                     AVKPTLRDELLFGFLEGKYKTHMVNTKIESFDFSNAQAGDQPLIIKEKAEFLKIFKSA
                     FTVRYAKVEFESRQGTEEIYKLLSSVNKNLGQARFQLDSQGRVLSLKITQ"
     gene            6197..7432
                     /locus_tag="VIG33_09185"
     CDS             6197..7432
                     /locus_tag="VIG33_09185"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY8271050.1"
                     /translation="MVIRDLSFSKASPAVASHLSTFAELPLAPVTDRFIALILDFLIF
                     SPVVSFFIASILRNLKTVLLLNSESEEALIIWALFLAGIVVVSSFLQALFMYFWQATP
                     GQKFMQLQVSSYPQRVHQLSFAQCLVRAFGWWGGLLMLGVPFLEILGHPLRRAFHERV
                     SDTIVLSHKQEAADFPLAMETHYVSSVMWVFYGFMLLLGLALMGKTYQAAVYEGLNGR
                     AILSQASCSNISVDKYKDQKRLDLALALYFTDEVDETCVFTEAQKAVWTLGGEEKSLG
                     QLAMALISDDEMEASAYRAKVCEESEKSEACAMSNYLLSEDEDRGNGLRRAGLGLVSS
                     RVLLLKDSMELNNYISAVDLIKDLEAEPSLRSFLDKNMVKAAWALNEKANSQGKSRRP
                     ASAEEKDILTGFKKRYDIE"
     gene            7429..8430
                     /locus_tag="VIG33_09190"
     CDS             7429..8430
                     /locus_tag="VIG33_09190"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY8271051.1"
                     /translation="MILPQPEPLSKFHRYPLTWALLALNVVIYFVFFAGPPTGVPGVF
                     RAQKILETNSTIVTGKLYYQFLQSLSTSEQKSRASWVGKLSFQNDDHMEILGSYALRD
                     GDFLSQSQTLKFHGDEVAIEKWRREIEAFKKFYDEDTIYNFGLNRAKIFSITWLTYQF
                     SHASLTHLFSNMLFLVVIGSAVEALVGSFLLVLYLLGGVAGGIAFLYYNGQGVVPMVG
                     ASASISALLAFYMIAETRRRVRYVYFISPYPGHNGFIYLPTLLIVPLFLLVDFTSLIA
                     NPEGIGGGVAYSAHVGGTLLGLVAGAFTRWGLRIERPLSFEEPIPQVEQNHDGECSP"
     gene            complement(8384..11332)
                     /locus_tag="VIG33_09195"
     CDS             complement(8384..11332)
                     /locus_tag="VIG33_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEY8271052.1"
                     /translation="MSDTQAQILSKGEEILKNMEGHSKSSIFSKDFWYGSIMEWSMKN
                     EKFKTNMFRFVDVLPSLNSGNEVARHLKEYFSENGEELPSVFNVGLGLGSLAPGFMAG
                     AIRKNITGMAKMFITGETPDEALPVLKKSRKNKMTFTVDILGEATLSEKEAQAYQSKY
                     RELIDWLVKDSQKWEEIPQIDRDHEGALPKVNVSVKMTALYSQINDKSWDETKNILKE
                     RLRFIFRAGMEKNIFINLDMEQYSVKHMTVEVFTELINEPEFKNYKFFGIVIQAYLRD
                     SFEDVIVLTEFAQKRAVPFWIRLVKGAYWDYEVIEAEQHGWPIPVYTSKAESDANYER
                     CAKYLLENIKFLRPAFASHNVRTLAACIVHAEKLGIPKAALEFQMLYGMAEPIKKALV
                     DMGYRLREYAPVGELIPGMAYLVRRLLENTSNESWLRGKFAEGKTTAELLKDPAQGLT
                     PTSSEVPKKPGRFYNEPLLDFAIKTDREKIQKALQEMKISLPLQVPLIINNQESSSQK
                     TLDRVNPSHLEQIVAKVHLATSQDAENAIQSAHTAFKTWKNTPAENRAALVDKLADIM
                     VRNRFKLIATQVYEVGKPWAEADGDIGEAIDFCRYYAKHMRELNRPQKMGNTPGESSQ
                     YIYKARGVTAVIAPWNFPLAILCGMVTAAAVTGNTVVMKPAEQSSLVALGLMKMIQEA
                     GFPPGVINFLPGLGEEVGEYIVNHPLTTTIAFTGSKEVGLHILKQATVVRPGQTHVKR
                     CIIEMGGKNAVIIDNDADLDEAVSGVLYSAFGFSGQKCSAASRVIVLDEVYDRFVDRL
                     VASAESIHILPAENPMAYMGPVVDKEAHERILATIAEAEKTHKTLFKANVPTGGFYAP
                     ATIFGDVAGDAKVAQQEIFGPVIAVIRARDLEHALIMANSTEYALTGGLFSRSPANIN
                     KIREGFEVGNLYINRGITGAMVDRHPFGGFKMSGVGSKTGGPDYLKQYMEPTCITENT
                     LRRGFAPLEE"
     gene            complement(11511..11999)
                     /locus_tag="VIG33_09200"
     CDS             complement(11511..11999)
                     /locus_tag="VIG33_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEY8271053.1"
                     /translation="MHKPQKNHSVRVELTFTQEEYEILQQTKSLLSHSCPQGSLSEVI
                     VHLANKLVKTKLGKTSPNSTQCNTEAEEKAKTPQKVKCQKNSREFISVRVRRILLKKA
                     HHCCEYVHPQTGVRCTSKYQLEIEHIKPVAFGGGNELENLMILCRTHNLQAAENLGLR
                     SH"
     gene            complement(12080..12445)
                     /locus_tag="VIG33_09205"
     CDS             complement(12080..12445)
                     /locus_tag="VIG33_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271054.1"
                     /translation="MDFHKTSDQDLLARLEKLARTERKLTHMILCHIHEAESRRLYAE
                     LGYGSMLAYLIQHLSYSESAAYRRLQAARLLKQTPSVAVKIESGALNLSQLSQMQKCL
                     KEECKKQEKVFLKSARKKF"
     gene            complement(12785..13120)
                     /locus_tag="VIG33_09210"
     CDS             complement(12785..13120)
                     /locus_tag="VIG33_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271055.1"
                     /translation="MKFVLDTGSNANWLFHKSQNLELISCGQAQNEKTTATCGLGNIS
                     VHRSLHFSEIKIGGYTANEIKFLCANENDFGGPSVTVEDGIIGTGQIAEWFNGIQIID
                     FISNKFFLH"
     gene            13734..14219
                     /locus_tag="VIG33_09215"
     CDS             13734..14219
                     /locus_tag="VIG33_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271056.1"
                     /translation="MRFLVLSFSICSLLSAQVRAQEFASEEPQAQQRLDFDEFENVTK
                     LLYAGKLAPHMKCDLKVRTIKEERKFSDGKKLVEVMEVIYYPRGLFADLKVKVLIPAQ
                     LATFGLKYVSNQWSGAGEDIKIEAHDSYDHWLRFMHDGKGNIVQFSLGNSLATYPCLI
                     K"
     gene            complement(14245..14748)
                     /locus_tag="VIG33_09220"
     CDS             complement(14245..14748)
                     /locus_tag="VIG33_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY8271057.1"
                     /translation="MDTWDAYTKTTLSEPPEFTWAREGNRVALAEYFRKGGSVNTKNH
                     KGYSLLMLAAYNDHWSTCAWLLQKGADVNSIDISGNSVLMGTCFKGHLRIIKLLLSSG
                     ADPFMQNCYGMTALDLAKAFGRLEVVRILTEEPPIPADHLAKVVKLISRRITSSNRER
                     CRDDSLG"
     gene            complement(14905..15321)
                     /locus_tag="VIG33_09225"
     CDS             complement(14905..15321)
                     /locus_tag="VIG33_09225"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY8271058.1"
                     /translation="MESPLTLPPKPHPCLSCGACCASFRVAFYWREAEPDGIWQVPPG
                     LTVDLDSTYRCMKGTEVKHQNKCAALDGRIGKRAHCTIYENRPSPCRGFRASYEDGTP
                     HPRCDEARKAHGLSPLRREDYPPSIPVSAHNDKKFP"
     gene            complement(15306..15908)
                     /locus_tag="VIG33_09230"
     CDS             complement(15306..15908)
                     /locus_tag="VIG33_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY8271059.1"
                     /translation="MTIRNIWAVGRNYAEHAQELDNEIPNQTDSEPMVFLKAGSTAVI
                     NSKEFKIPAWAQNVHHEIELALQFNEHLKIDKAALALDLTERTWQQKLKAKGSPWTLA
                     KSFTNSCPLSDFFTVDSLEELKNLNFSLTVNGELRQKGNTSQMIFPVPQLVEYILLHF
                     PVCPGDLLLTGTPSGVAALQRGDQLVAELPGKVHKQWKAL"
     gene            complement(15905..16399)
                     /locus_tag="VIG33_09235"
     CDS             complement(15905..16399)
                     /locus_tag="VIG33_09235"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEY8271060.1"
                     /translation="MKVYLFRHGQKESMPFKDPDLTNFGHQQAAKLAELIRNGALLRG
                     TQFLTSPRLRAQSTLKPAAQLCGSLVQVAQDLDQRTHFESHEVFRARIQHLLNSLENR
                     FSQKDVVYLCSHHDWIEESLSVIPADTDLLDPRYWSWRPAQYMYFEIVDGLWILKHFN
                     RIEP"
     gene            16465..17373
                     /locus_tag="VIG33_09240"
     CDS             16465..17373
                     /locus_tag="VIG33_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEY8271061.1"
                     /translation="MKEKVHRFVSNDFTIRIAAVNSTAVVAEMQKLQQTYPLPTVAVG
                     RAMTGALLLASHLKEDQQVGLYFKGSGPLTAVYAEASFEGKVRGYSPVPAFEPENYDN
                     GLKLAPYMGQGMLTVTRHLPFQKQPHNGTVEMVSGEIGDDIAHYLIQSHQIRSLVSLG
                     VYLDTYGQVRAAGGVVLEVMPGADESLVKKIEKNAEAFKENISKMVLNGATALDLVTP
                     YMEGIPFTEIEHAHAIEYFCPCTKERVVRAIETLGIEDLNDMILKNEAAEVVCQMCGR
                     PYEVPVEDLVELLVELKEKLTKSGLH"
BASE COUNT         4770 a         3640 c         4197 g         4978 t
ORIGIN      
        1 ttcaacaata tcttaaagaa gaattaaata gtgtaaattg ttggttttgt tattttttta
       61 tccacttgtc atattgtgtc atgcggagtt ttaggttata acctttctct gttaacgaac
      121 gaaaaaaaaa tggggagact gggcaccgtg atcgattcag ggacaaatcc gaatgctatt
      181 aaagaagaac tgatggatga actcgatgaa gaactggacg aggatcttga tgaagatgaa
      241 aaagtcatag atcagtttgg ggatgagact ttagctgctg atgctcttga aggtcagtta
      301 gctttgaatc catcgagtca acttggtgaa gaagaagaag atataagttt ggatgagatt
      361 gataacatcc ttgaacttaa ggatgataag tatccaaagt tcacgcttgc caaaaataaa
      421 gctcgttttt taagaatggt gagctggtat cgcgctaaag aagaatggat tgaagtggct
      481 cctttaagtg gagtgaccaa acttttcaag cagcaaacca aagagttgga agggatccgt
      541 tcttcgaaac tggattacga aatggaatta gagacaggga cattgactcc ttctcaaaga
      601 tcttatcgtc gtgatgaatt aaaaatgtgt cgtgttcaag aaaaaatggc ggttcatttg
      661 atttccaagt tacaagttaa gatcaaaagc ggccgtagat aatcatcaaa agccagggcg
      721 gaggttcttc agggggatct tcgccgttcg gtaagttatt ttactgcgtt gatcaacgct
      781 ccacctttaa gcatagctat gattctattt ctcagtacca tgctcactgc ggtacacccc
      841 caactgcgtc cctgaataat attttcttct ctgacgtagt cagctccatg aaggacaatg
      901 gcgcgactgc gagcatgtga attggtggaa gatcttccat cgagacgcaa agataaacca
      961 tgatttccgt aataagtttc agcggtggtg taaaagccta tcgagctggc attcgttcca
     1021 gattgattgc tgaacctttc ggcgtaacca tcgtgatcca catcggaacc tttaccatga
     1081 gcgacatgaa tcgcttgcac agagccggtt ttcatatcaa cgatgtaaaa tcgtttttct
     1141 ttcgaactct gggaaaaatt aatgaccgcg atataatttt ggtttttaat tttggattta
     1201 tttttttcga agtagaggac cgtgtccgat aaatccttac tgggaacgac tctgtttgga
     1261 tccaagtaat catacttggc gagaatactt tctctttcct gagaactcaa aacaggaggt
     1321 agttcaacca gtacctgttc cacttgtacg gcagctgcgt cagcaggttg ttcttcggta
     1381 ggatcgtcgt ttaatcttgg agtatcaaag ttgcttccac aagctgtgag gccgaatatt
     1441 ataccgcaaa tcataatttt ctttgagcat atttttatca ttggatttcc cctcaaaagt
     1501 ttcggtatac gaaaaaaggc tacaagaggg tgtcggtgaa gagggaaaaa atattgacta
     1561 aacatccaag attaggacct aagcctaagt aaaaacaaaa cttaagtcgc gtgaataaac
     1621 gaatcacggc actgaatcac ggcacaggcg agtgacggga aaagtcactt ataaaagagt
     1681 tagacaacaa aaaaaaggcg acacaaggat tatttcgaga tgaaggtttt tctcattttg
     1741 gcacaactca tgctaacgaa ttaaggtggg agagtactat ggaacgtaga ttgatattgc
     1801 tactattatt agtatttctt atgacatcgt gtgcacagaa tggttttcaa gaaccacttg
     1861 catcagtgca agaggctccc gcagtcacga cctattctcc acccataccc actaagaaaa
     1921 tagttaagac ccgtgaaatg gttgatggcg cttcccaagt ttccgacttc aaacttaata
     1981 tcgacgggcc aaaggcaacc tcagaattga aaggaacctt cagttttcag cctgcggatg
     2041 gcaatcgtat agaagttccg gtggatgttt ccggctcgat tgaagaatac aattccttta
     2101 tgaagccgac taattccgat cgtttgaaag taatggattt acaatcgggc gcgaagctaa
     2161 cttgtcttac caaggactgt acagagtcct ttgtggatat ttacgtggtt cataagggag
     2221 tcatttacca tcaccaggtc gaagcgaaag gggcccagcc tacgactcag cagccagcca
     2281 cacctgttaa agaatctcct gccaaagata ctttttcggc gaattcaaaa gtaaaagagc
     2341 agaaggttgg agatcaaaaa gttgcggatc cgaaacctaa ggatcaaaaa gcgacagagc
     2401 caacgaaagt tccatcggaa gaagccacag aagaagccac agacaaagct gatgatggct
     2461 cccaatatga gcatgatatt gatgaggaag cagaggcagg aacctatgtc ggagagcctc
     2521 agcgtgatat tgaggtttta tttccagatt tggatgccaa tactggcaag gcagacgcag
     2581 gcaaagatca agagtcgaaa aaagatgaga aaaaagacga taaaaatata cctccttcaa
     2641 aggatatcaa tatacaggca aagccgccga aaactctgcc tgcgcaaggt tccagcgctt
     2701 caaggttgat caacgttatg gtgaaagggc tgggtcaagc tgtcacggcc ctgggaacgc
     2761 gaaacctggt gaaagggcac ctggaaaatc ctacggatat ttttaattat gaagagtcga
     2821 atcctcaagt cgggtttcat attctctatc cgaaaagaaa agcctatttt gggacggatg
     2881 atatgttggc gatgttaagc aacatgggac gttatagtcg aaataaagtg aacgggtatg
     2941 ttttaaatgt cggggatatt tcggcaaaga atggtgggcc attaggtaag cattcctctc
     3001 accaaatggg tcttgatgcc gatatctcct attattttga tgcaaaagaa aaacaaaaag
     3061 gcttggtgga tgcggtgaaa tcgtccagac cacttcgtag ctttatggct gaggagcagt
     3121 gggagatgtt caaaactttt gttggacaag gtaacgtgga tcgtatcttt atccaccccg
     3181 ctcttaaaaa agaactttgt ggagtggcgg cgaagaaagg ggatttgaac tcgagcaatg
     3241 ggacagcgtt tgaaactctt cgagtcatgc gtcctgaagt tcatcatgat aaccacttcc
     3301 atcttcgttt gaagtgttcg acagctcagc cacgctgccg ccagatggct ccaccagcga
     3361 aagggacggg ttgttagagt cgtttatacg gacttgagcc cagaaccact tttttaataa
     3421 atcaagcctc aatatgagat tgggggattc taaagtcatt ctcaatgctt aaagtgtctc
     3481 gaaatatttt ttctaggccc ccttaattct cacgatgaaa cgccgatacg ataagtgtga
     3541 ttcaatcatg aacgtttaac gtatcaatag gggagggacg aaatgtctcg tacaatgatg
     3601 attatggtga gtgataatgt tgaggccatc gaaaatgcca agaaacaatg ggaacaccaa
     3661 gatgtgactt tccaagttta ttcacctact caatggagag aaggaatgga atcttcgttc
     3721 ttccgtcaac aactgatggc tggagttccg gctttggctt tcggctcgag ctcagtcagt
     3781 ggcgaagtcg gtggggccaa tattttggcg ttccccgctc ccagctctac ttacaacacc
     3841 acggatatta aaattagaaa aatggatgaa ctcgaagcgc atgcgatcga gaatgcgatt
     3901 gttcagtaca aaggcaattt gactgaagcg gcaaaagcat tgggtattgg tcgtgccact
     3961 ctttatcgta aagtgaagca atatcatatt gatccatctc aagctcgtaa gaaacgtgca
     4021 gcttaattat tgagtcgatg aggtttagtt tcttcaaaaa gccaggaggg actcctggct
     4081 ttttattttt tatcctctgt atgggattag gttttgcggc ggccaggaga agccttccag
     4141 taaattccta tgcccggatc tgccatttgg tgatggagca tatttatctc aacgatgagc
     4201 caatgaaaat ttggaataaa tcctgtcttg ctcgttctga actggttcgg aaaaacactc
     4261 ccgtcgcgga tattatcaag gatctcaatg agcagttttc tcttctgcaa acttcacatt
     4321 tattgattta tgatgctgac gaatccaaaa aggtctggag tggtgaaagc aaagagactg
     4381 gcatcgaatc ccagtatgtt gatggcgagc tggtgatttt gaaagtgcat cgcaattctc
     4441 cggcggagtt ggcaggacta cgcatgggag acgtgatcta tcgtattaat gaggaattgg
     4501 gaacgccgga aatggcggaa aaaatttctg gaggctatct cattcagcgc cataagaaaa
     4561 tcacggaata taaaatccaa gtcaagacga tccaacgcga agaagagccc gagatcgggc
     4621 ggatctccga ccgtacggtg atgctgaagg tgccttcttt tcgcgctgaa ttttttgacc
     4681 gagataaatg gaaaagacaa gtgactgaac ttaaaaaata tcaaaaaatc attattgatc
     4741 tcaggggtaa taacggtggt aattttgtgg cggggctccg ccttttatct ccattcatgt
     4801 gtgggcccca agatattggt tatttgtgga agccaaaatc aaagtttaaa aaggatgcgg
     4861 tttttgatga taaccttgat gatgaaagtc aggtgaatat tcttgatgaa tcctttttag
     4921 tcagactttt aacctttgct gattacgatt gtttaaccgc ttcagtcgcc gttatggtgg
     4981 attcgggcag tgcctccacg gcagagatgg tggctcaagc cctcaaggat tatatcggag
     5041 cgaaaatttt cggttcagcc agcgctggtc agctcctcgt gggggtatgg tatccggtcc
     5101 cagaacttgc tgaaggagtg aggatctcga ttccagaggc ggtttatcaa actcggcgtg
     5161 ggcacaaaat cgagggccca ggagtccaag tggacaaggt cctctactat catttagaag
     5221 aactgcagaa cggggaagac agctggctta aacatgcagt gcagttgttt tagcaaagct
     5281 ggaatgatgg actctagttc acggacttaa ggcctgcaat gagaaccagt ctgttgatga
     5341 gctgtctcat aatagaacgt ctaacaactt acagtcggat gttgagtgcg tgtacgataa
     5401 gatgtatttt aaaagaacct agtgatttaa aaaatcggca tgagtttcgc aatatctcaa
     5461 tatagagcta accataaccg aaaattcatg tgtctcaaat gaggggaact agaaatgtcc
     5521 aaaagcactg ttgaaaataa agtactggaa tttccgaaat ctcaatcctt gcgtaagcgc
     5581 ctcttagacc gtggacaaga tcaaaaaacg gttttaagtg tctctattat ttctgtattg
     5641 gtgatcacgg tttttcttaa tgagtggatt gtgaagtctc aacattccgt gaattccctt
     5701 acagagagcc gccgcggtat cgccagcttt gaagatcgtg attcggttga aggtatcaaa
     5761 tgggaacatg aacttgcgca aaaactagga caagaaaaag atacatcaaa aagtttctcg
     5821 gcggtaaaac ccacgctcag agacgaattg ttatttggat ttcttgaagg aaaatataaa
     5881 acgcatatgg tgaacaccaa aattgaaagt tttgatttct ctaacgccca agcgggcgat
     5941 caacccttga tcattaaaga aaaggctgag ttcttaaaga tttttaaaag tgccttcact
     6001 gtgaggtatg ctaaagtcga gtttgaaagt cgacaaggca ctgaagaaat ttataagctc
     6061 ttaagctcag tcaataagaa tctggggcaa gctcgcttcc aactggattc acaaggtcga
     6121 gttctttctt taaaaatcac tcaataacta tcattcctag accgcagttt taagttcttt
     6181 gaaccgataa tcccttatgg ttataaggga tttatcattt tcaaaagcaa gtccggcggt
     6241 agcgtctcat ttatccactt ttgcggaact tcctctggca ccggtgaccg atcggtttat
     6301 agctcttatt ttggattttt tgattttttc acctgtggtg agtttcttca ttgccagtat
     6361 tttgagaaat ctaaaaacag ttctgctgtt aaattctgaa tccgaagaag cgctgattat
     6421 ttgggcttta tttcttgcag gaattgtcgt cgtctcctct tttttgcaag cgctattcat
     6481 gtatttctgg caggctactc ccggtcagaa atttatgcag cttcaggtct cctcttatcc
     6541 gcagagagtt catcaattga gttttgcgca atgtttggtt cgcgcttttg gatggtgggg
     6601 aggcctcttg atgttggggg tgcctttcct ggaaattctt ggtcatcccc tgcgtcgtgc
     6661 ttttcatgaa agagtttctg acacgatcgt actttctcac aagcaagaag ccgcggattt
     6721 ccccctggct atggaaacac attatgtgag ttccgttatg tgggtttttt atggtttcat
     6781 gcttctctta ggcctcgcac tgatgggaaa gacctatcag gcagctgtgt atgagggcct
     6841 caatggaagg gctatccttt cccaagcatc ttgttcaaat atttccgtgg ataagtataa
     6901 ggatcaaaag cgtttggatt tagcgctggc gctttatttt accgatgaag tggatgaaac
     6961 ttgcgttttt acggaggcgc aaaaggcggt ttggactctg gggggcgaag aaaaatctct
     7021 aggacagttg gcaatggcct tgatcagcga tgacgaaatg gaagcttccg cttatagagc
     7081 caaggtttgc gaagaaagtg aaaaatctga agcctgtgcg atgtcaaatt acctactctc
     7141 agaagatgaa gaccgcggca acggcctgcg ccgcgcgggg ttagggctag taagttcaag
     7201 ggttttgctt ttgaaagatt ccatggagct caataattat atttcggccg tggatttaat
     7261 taaagacctt gaagcggaac ctagtttgcg ttctttcttg gacaaaaata tggtgaaagc
     7321 tgcttgggcc ttgaatgaga aagcgaatag ccaggggaaa tctcgcagac ccgcgagtgc
     7381 cgaggaaaag gacattctga cgggctttaa gaaaagatat gacatcgaat gattttacct
     7441 cagccagaac ccctttcaaa gtttcatcgt tatcctctga cctgggcttt gttggcctta
     7501 aatgtggtta tctattttgt gttttttgcc ggtcctccaa cgggtgttcc aggcgtcttc
     7561 agggcacaaa agattttgga aacaaattcc acgatagtga ccggaaagct ctattatcaa
     7621 tttcttcaat ctttaagcac cagcgagcag aagagccgag cttcttgggt tggtaagctc
     7681 agttttcaga acgatgatca tatggaaatt ttggggtctt atgctcttcg tgatggggac
     7741 tttctcagtc aatcgcaaac gctgaagttt cacggcgatg aggtggccat agagaaatgg
     7801 cggcgggaga tcgaagcctt taaaaaattc tatgatgaag atacgattta taactttgga
     7861 ttgaatcgag ctaagatttt ttcgatcact tggctgacct atcaattttc acatgcaagt
     7921 ttaactcacc tttttagcaa tatgttattt ctcgtagtta tcggaagtgc ggtggaagct
     7981 ctggtcggca gctttttact tgtgctttat ttgctgggcg gggtggccgg ggggattgct
     8041 tttctgtatt acaacggcca gggggttgtc ccgatggtgg gggccagtgc ttcaatcagc
     8101 gctcttttgg ctttttatat gatcgctgaa acgcgtcgtc gcgtgcgcta tgtatacttt
     8161 atttcgcctt atcctgggca taatggtttt atttacttac ccactctttt gatagtgcct
     8221 ttgtttttat tggtggattt caccagtttg attgccaatc ccgaaggcat aggtggcgga
     8281 gtcgcttact ccgcccatgt cggtggcacc ttactcggac tcgttgccgg agccttcact
     8341 cggtgggggc tacggattga gcgccccctg agttttgaag agcctattcc tcaagtggag
     8401 caaaaccacg acggagagtg ttctccgtaa tgcaagtcgg ttccatatat tgtttcaagt
     8461 aatcgggccc gcccgttttt gaacccacac ctgacatctt gaagccgcca aaaggatgac
     8521 ggtcgaccat ggcgccggtg atgccacggt tgatatacag attgcccact tcgaatcctt
     8581 cacggatttt attgatgttt gcagggctgc gagagaaaag tcctccggtt aaagcatatt
     8641 cggtactatt ggccataata agagcgtgct ctaaatcccg cgcccgaatg acggcgatca
     8701 cgggaccaaa gatttcctgt tgagccactt ttgcatctcc ggccacatct ccgaaaatgg
     8761 tggctggtgc atagaagccc cctgtaggta cgttggcttt aaaaagagtt ttatgggttt
     8821 tctctgcttc tgcaatggtc gcaagaattc tttcatgagc ttctttgtcg accacgggtc
     8881 ccatataagc cattggattt tctgctggca aaatatgaat actttcagcg gaagccacga
     8941 gacgatctac aaagcggtcg taaacttcat ccaatacgat cacgcggctg gcggcagagc
     9001 atttctgtcc gctgaagccg aaggccgagt agaggactcc actgacggct tcatcgagat
     9061 ccgcatcatt atcaataatc acggcatttt tgccgcccat ttcaataatg cagcgtttga
     9121 catgggtttg tcccgggcgt acgacggtgg cttgctttaa gatatgaagc ccgacttctt
     9181 tggagccggt gaaggcaatc gtcgtcgtca aaggatggtt cacgatgtac tcacccactt
     9241 cttcacccag gcctggtaag aagttaatga ctccaggggg gaaaccagct tcttggatca
     9301 ttttcataag acccaaagcc accagtgaag actgttccgc cggtttcatc accacggtat
     9361 tcccagtcac tgcggctgcg gtgaccatgc cacataaaat cgccagggga aaattccacg
     9421 gagcgatcac ggcagtaacg ccacgggctt tgtaaatgta ttgagagctt tctcccggag
     9481 tattgcccat cttctggggt ctattcagtt cgcgcatatg tttggcgtaa tagcgacaga
     9541 agtcgatggc ttctccgata tccccatcgg cttccgccca gggcttgccc acttcgtaaa
     9601 cttgagtcgc aatgagtttg aatctattgc gaaccatgat gtcagccagt ttatccacca
     9661 aagcggctcg gttttccgct ggagtatttt tccaggtttt gaaggcggtg tgggccgatt
     9721 gaatcgcatt ttccgcatcc tggctggtcg ccagatggac cttcgcaacg atttgttcaa
     9781 ggtgagaagg attgacccga tccaaagttt tttgcgagga actctcctgg ttattgataa
     9841 tcaaaggaac ctgaagcggc agagagatct tcatctcctg cagggctttt tggatttttt
     9901 cccgatctgt tttaatcgca aaatccaaaa ggggctcatt gtaaaatcgt cccggctttt
     9961 tgggaacttc actcgaggtt ggcgttagac cttgtgcggg atcctttaaa agctccgctg
    10021 tggttttgcc ttcagcaaat tttccgcgca gccaagattc attggaggtg ttctctaaaa
    10081 gccgacgaac caagtaagcc atgcctggaa tcaattctcc caccggcgca tactctcgca
    10141 ggcgatagcc catatctaca agagcttttt taataggttc ggccatgcca taaagcatct
    10201 ggaattccaa ggcggccttg ggaataccca atttttccgc atgcacaata caggccgcca
    10261 gagtcctcac gttatgagag gcaaaggcgg ggcgaagaaa tttgatattt tcgagcaagt
    10321 acttggcaca acgttcatag ttggcatcgc tctcagcctt actggtgtaa acggggatag
    10381 gccagccatg ctgttcagcc tcgatgactt cgtaatccca ataagcccct tttactaaac
    10441 ggatccagaa aggaaccgcc cgtttttgcg cgaactctgt caagacgatg acgtcttcga
    10501 agctatcgcg gagataggct tggataacga tcccgaaaaa tttatagttt ttgaattcag
    10561 gctcattgat aagctcagtg aaaacttcca ccgtcatatg tttgacggag tattgctcca
    10621 tatccaaatt gataaagata tttttctcca tgcccgcgcg gaaaataaaa cggaggcgtt
    10681 ctttcaaaat gtttttagtt tcatcccaag atttatcgtt gatttgcgaa taaagcgcgg
    10741 tcatttttac ggacacgttc actttcggca aagcaccttc atgatcgcga tcgatttgcg
    10801 gaatctcttc ccatttctga gaatctttca ccaaccagtc aatcagttcc ctatatttgc
    10861 tttggtaagc ttgagcttcc ttttccgaca aagtggcttc acctaaaata tccacagtga
    10921 aagtcatttt gttcttccgt gattttttta aaaccggcag ggcctcatcg ggggtttcac
    10981 cggtgatgaa cattttagcc atgcccgtga tatttttgcg gatggctccc gccatgaagc
    11041 ccggagcgag agaacctaag cccaggccca cgttgaagac agagggaagc tcttccccat
    11101 tttcagagaa gtactccttt aaatggcgag ccacttcgtt gccggagttg agggagggca
    11161 agacgtcgac gaaacggaac atattggttt tgaatttttc gtttttcatg ctccattcca
    11221 tgatggagcc gtaccaaaag tctttagaga aaatggagga tttggaatgc ccttccatat
    11281 ttttgagaat ttcttcacct ttagatagaa tctgcgcctg tgtgtctgac atatggtttt
    11341 acctcaattt ggaggtccgt atgttctctc tgagagaaag aaaactcaag gcttttgcga
    11401 aacggcacag ggccttgagg aagtgaggcg acgcaacgca gctctgggag agcgagagtc
    11461 ttgcgaaatt ctttattatt actgctatca gatttcatta ttaaaatctc tcagtgcgat
    11521 cttagcccca gattctcagc ggcctgcaaa ttgtgagtgc ggcagagaat catcaaattc
    11581 tccagttcat tgccgccccc aaaagcgacg ggtttaatat gctcaatttc tagttggtat
    11641 tttgaggtgc atctcactcc tgtctgaggg tgaacatatt cacagcagtg atgggctttt
    11701 ttcaaaagaa ttcttctgac tctgacagaa ataaactctc gggaattttt ctgacattta
    11761 actttctgag gagtcttggc cttttcctcc gcttcggtgt tgcactgtgt ggaatttgga
    11821 gaagtttttc caagctttgt tttaaccagt ttatttgcca gatgcacaat cacttcactg
    11881 agagaaccct gaggacaaga gtgtgataaa aggctttttg tttgttgtaa aatttcatac
    11941 tcttcttgcg taaacgtcag ctccacacga acggaatgat tcttttgcgg tttatgcact
    12001 tcttgggttt tgacagggcg gtcaaactct tgagcgagga ctttttcagt ttcaaaagtg
    12061 gtcttatttt ctaggagtcc tagaacttct tccgtgcgct cttgagaaag actttttcct
    12121 gttttttaca ttcttctttc aggcattttt gcatttggct taactgggaa aggtttaagg
    12181 caccggattc aattttcact gccacagaag gagtttgttt taggagacga gccgcctgca
    12241 agcgacgata ggccgcggac tcgctatagg ataaatgttg gataagataa gcaagcatac
    12301 tgccataccc cagttccgca tagagtctgc gagattcagc ttcatggata tgacaaagaa
    12361 tcatatgcgt gagctttcgc tcggtgcgcg cgagtttttc gaggcgggcg agtaagtctt
    12421 gatctgaagt tttatgaaag tccatgctgt gctccttgtt ttatagagag gaacatacca
    12481 tggtttttag aaagtgattt ttgcaccttt ttctaaaaca aaaaagaatt gaagctctct
    12541 gctaatgctt tgtaggaagc gcgaaatggc tccgcaataa gagtattttc cataaacgcg
    12601 tcgggtcctt ctttaaagag gatcgccttg tagtgaagcc ttcggtgtga cacgtttacc
    12661 tcgcaagtga aaaatgcatc aactcaaaaa ataatgcttg gcaaaagcga agctgtgttt
    12721 gactttaatt aaagtcatta ttttttaaga ttgagcttag aaaactataa gtaggcggaa
    12781 aactctaatg tagaaaaaat ttatttgaaa tgaagtctat gatttgaatt ccgttaaacc
    12841 actctgcaat ttggccagtt ccaatgattc catcttcgac cgtaaccgac gggccgccaa
    12901 agtcattttc atttgcacat agaaacttta tttcattggc agtatatccg ccaattttaa
    12961 tctcagaaaa atgtaatgat ctgtgaactg aaatattgcc tagtccacaa gtcgcagtag
    13021 ttttttcatt ttgcgcctga ccacaactga tcaattctaa attttgactt ttatggaaga
    13081 gccaattagc attagatcct gtatccagga caaacttcat tattttttta cctatgccta
    13141 aatgaataac tgggcgaccc cttgaaaaag agattttcga gcaatcttgc tggttatcat
    13201 ttgggaatat tgaatttgta aaatgaataa gttgagcttg ataatttatt cataagcagt
    13261 gacttcgtag ttctgaagtt ccaatataga tttccggtgc ttttagattc ttgactatct
    13321 tagtacgtac ttttagcggt ccaaattcta ttccatttat tatcagcgaa tcgagaaaag
    13381 aaaatccctc ttgagaaagg ggaatgtttt gaagatttga ttcctcgggc aacttcaata
    13441 cggttattgg ggcacctgtg tcgagtacgc aacgctcaca atgagttccg tttataatac
    13501 ctttcattac aggatcacct tgaaagagaa acggaagact aaagtaatcg ggtaatttgg
    13561 gaaactgcat agatggacaa cttactctga ccaagtcagt tgtcaagaaa ggagcctcat
    13621 tcttcaggcc ttgtaacata attcttggta aattcttatt tttctcatta gtttaattga
    13681 ttggaccgat acaattaaat ccaatattaa aattccatcc aaaggagaat tttatgcgtt
    13741 tcttagtgct gagtttttca atttgttctc ttctatctgc tcaagtgaga gctcaagaat
    13801 ttgccagtga agagccacaa gcgcagcaaa ggttggattt cgatgagttt gagaatgtga
    13861 cgaagcttct ctatgccggg aaattggcgc cccatatgaa gtgtgacctt aaagtccgca
    13921 ccatcaaaga agagcgaaag ttttccgacg ggaaaaagct cgttgaggtg atggaagtta
    13981 tctattatcc acgagggctt tttgcggatc tgaaagtgaa agttttgatt ccggctcaat
    14041 tggcgacctt tggattgaag tatgtgtcga atcaatggag tggagccgga gaggatatta
    14101 aaattgaagc ccatgatagt tatgatcact ggcttcgttt tatgcacgac ggaaaaggca
    14161 atattgtcca gttcagttta gggaatagct tagcgaccta tccttgcttg ataaagtaat
    14221 cctactgagg ggaggacttc ctcattatcc aagggaatcg tctcggcatc tttccctatt
    14281 cgatgaagtg atccggcgtg aaatcaactt caccaccttg gctaagtggt ctgctggaat
    14341 gggcggttct tcggttaaga tccgcaccac ttcaagacgg ccaaaggcct tcgctaaatc
    14401 caaagcagtc atcccataac agttttgcat gaaaggatcc gcgccagagc tcaagagcag
    14461 cttgatgatg cgaagatgtc ccttgaaaca agttcccatc aagacgctat ttccagaaat
    14521 atcaatggag ttgacgtcgg cgcctttttg caaaagccag gcgcaagtgc tccaatgatc
    14581 gttgtaggct gctagcatca aaagagagta gcctttatgg ttttttgtat tcactgagcc
    14641 gcccttgcga aaatactccg ccaacgccac acgatttccc tctcgggccc aagtaaattc
    14701 tggcggctca gaaagtgttg ttttagtgta tgcatcccag gtgtccatgt agcttctcct
    14761 tacattttag caacgtcgct tttgagaatc gacgttagct taaagaagaa aaaggagagc
    14821 tttgagttca attttttaaa aatgattaag tacaattatt tttttactta aaaggtcaca
    14881 acatcttgga aaacgaaaaa cctatcatgg aaatttctta tcattgtgag cagaaacagg
    14941 aatggaggga gggtaatcct cacgtcgcaa cggagagagg ccatgggcct tgcgggcctc
    15001 atcacagcgc gggtgaggag tgccgtcttc gtaagaggct ctgaaaccgc gacaaggtga
    15061 ggggcggttt tcgtagatcg tgcaatgggc acgcttgcca atccggccgt cgagggcggc
    15121 acacttattt tggtgtttga cctccgtgcc cttcatgcag cggtaggtgg aatccaaatc
    15181 cacggtcagc cccggaggca cttgccatat tccatcgggc tcggcttccc gccaataaaa
    15241 ggcaacgcga aaagaggcgc aacaagcgcc gcagctaagg cagggatgtg gttttggcgg
    15301 tagtgttaaa gggctttcca ctgtttgtgg actttccctg gaagctcggc gaccagttga
    15361 tccccgcgtt gaagggccgc gacgcccgag ggagttcccg tcagcagaag gtcgccgggg
    15421 cagaccggga agtgcagcag aatatattcc accaactgcg gcacagggaa aatcatctgc
    15481 gaagtattgc ctttttgccg aagttcccca ttcacagtca gggaaaaatt gagatttttg
    15541 agttcttcca aagagtccac tgtgaaaaaa tccgagagag gacacgaatt ggtgaaagac
    15601 ttcgccagcg tccatggaga acctttggct tttaactttt gctgccaagt gcgttctgtc
    15661 aaatcaagag cgagagcggc tttatctatt ttcaaatgct cattgaactg cagggccaat
    15721 tctatttcat gatgaacatt ctgcgcccaa gcgggaattt taaactcttt gctattgatg
    15781 actgcggtgc tgccggcttt taaaaaaacc ataggctctg agtcagtttg attgggaatt
    15841 tcattgtcaa gttcttgggc gtgttccgca tagtttctgc caacggccca aatgttacga
    15901 atggtcatgg ttcaatcctg ttaaaatgtt taagaatcca aagtccatcg acaatttcaa
    15961 aatacatata ctgcgcggga cgccagctcc aatagcgggg gtccaagagg tcggtatccg
    16021 ctggaatgac cgagagggat tcttcgatcc aatcgtgatg ggagcaaaga tacacaacgt
    16081 ctttttgcga aaatctattt tctaaggaat tcaataaatg ttgaatccgt gcccgaaaaa
    16141 cctcgtggct ttcgaaatgg gttctttgat ccaagtcttg agccacctga acaaggctgc
    16201 cgcaaagctg agccgcgggc ttaagagtac tttgagcgcg aagtcgcggt gaggtgagga
    16261 attgggttcc tcgcaggagt gcaccattgc gtattaactc tgcaagtttt gctgcttgtt
    16321 gatgcccgaa atttgttaag tcagggtcct taaaaggcat tgactctttt tgtccgtggc
    16381 gaaaaaggta gactttcata ctctcaataa ttgcgtttat ttcgagtctt tgatatcttt
    16441 gacactcatt gtctggagat tgccatgaaa gaaaaagtcc atcgttttgt ttctaacgat
    16501 tttacgattc gtattgcagc ggtgaactcc acagccgtcg ttgcggaaat gcagaagttg
    16561 cagcaaactt accctctgcc gaccgtagca gtagggcggg ccatgacggg agcgctttta
    16621 ctggcaagtc acttgaaaga agatcaacaa gtaggtcttt actttaaagg cagtggtccg
    16681 ctgacggcgg tttatgctga agccagtttt gaaggcaaag ttcgcggtta ttctccggtc
    16741 cctgcatttg aacctgaaaa ttatgataat ggtttgaaat tagctcctta tatggggcag
    16801 gggatgctca ccgtcactcg tcatttgccc tttcagaaac agcctcataa cggaaccgtg
    16861 gaaatggtca gtggtgaaat cggcgatgat attgcccatt atttgattca atcccatcaa
    16921 attcgcagtc tggtgtcctt gggagtttac ctggatactt atggacaggt ccgggctgcc
    16981 ggcggggtgg tcctggaagt gatgccagga gcggatgagt ccctggtcaa aaaaattgaa
    17041 aaaaatgccg aagccttcaa agagaatatt tctaaaatgg ttcttaatgg ggccacggcg
    17101 ttagatctgg tgactcccta tatggagggc attcctttca ctgaaattga acatgctcat
    17161 gcgattgaat acttctgtcc ctgcactaaa gagcgtgtgg tgcgagcgat cgagaccctg
    17221 ggtattgagg atctgaatga tatgattcta aaaaacgaag ctgcggaagt ggtttgccaa
    17281 atgtgtggcc gtccttatga ggttcctgtc gaggatttgg ttgaattgtt agtggaactt
    17341 aaggagaagt tgactaagag cggactgcac tagacttatt tccgatcaag tttccgatca
    17401 aggtttcgat gaagtttcta ggcagcgaag ttctggactt cgtaagggtc cagaatagtc
    17461 tagcatgtct caagcggctt cagcgacacg acgataaaat agacatatga ttcttcttcc
    17521 tctaggactg caaagtcgca tagtaaagtg gcccatcgcc accgttttga ttattgtggc
    17581 aacct
//
LOCUS       DATBTE010000252         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1604441, whole genome shotgun sequence.
ACCESSION   DATBTE010000252 DATBTE010000000
VERSION     DATBTE010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604441"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VIG33_09245"
     CDS             <1..736
                     /locus_tag="VIG33_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEY8271062.1"
                     /translation="TGDHILASNRTPGKLQKLTDHYKIQSMDNNESLVENSDIVILAV
                     KPQDLLTAIEPIASSFHDDQIVISLAAGINMKTLEKYLPNTRLVRMMPNTPSIIGRGV
                     IGYLLNEIDAALEAVVDDMCSPLGYTLKVEDEDQFEALMISCSSGTGFVLEMMMYWQD
                     WIVEHGFDEETAKRMTLETFMGASMLAAQSKDIPFEELQARVTSKKGVTAAGLQSMRE
                     LEIERALRISFEKAAMRNKEISRETK"
     gene            792..1322
                     /locus_tag="VIG33_09250"
     CDS             792..1322
                     /locus_tag="VIG33_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEY8271063.1"
                     /translation="MKITPIDIVHKSFNKRMLGLDADEVSDFMAQIASQMEALIQERN
                     TLKEALREKELALLDYKDRDQVLKSTIATASQMTERLRQDSEREAKLIIADANQKSEM
                     IIRDSRDSLKKMYQEITDLKRARMQFEANLKALVQAHLTLLEQGEKYMPQMQLPNYNI
                     TPSPGKTTEVSPLSAG"
     gene            1324..1617
                     /locus_tag="VIG33_09255"
     CDS             1324..1617
                     /locus_tag="VIG33_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEY8271064.1"
                     /translation="MFTQTERGIKLALFIQPKASKNEIIGPHNGALKIKITAPPVDGK
                     ANVALIEFLSDVLDIPKRQIEILKGETGRNKSVEIFGLSQEKIEQRLGLSGLN"
     gene            complement(1614..1952)
                     /locus_tag="VIG33_09260"
     CDS             complement(1614..1952)
                     /locus_tag="VIG33_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271065.1"
                     /translation="MKHLDVFFKNVDKAMDFQVLASEGLRFYKGPDAQEDLLKENQIT
                     GEPGTLRPGTPIMVLGKEKGFLKVAIININYDLMDSKNSVGYIQDTALVQAFLPENLI
                     AIVDGRDMIY"
BASE COUNT          615 a          486 c          467 g          567 t
ORIGIN      
        1 caccggtgac catattttgg cctcgaatcg cactcctggc aaattacaaa agctcactga
       61 tcattataaa atacaaagca tggataacaa tgaatcgctt gttgaaaact ctgacatcgt
      121 gattctggct gtaaaacccc aggatttatt aaccgcgatt gagcccatcg ccagttcctt
      181 ccatgatgat caaattgtca ttagtttagc tgcgggtata aacatgaaaa ctctggaaaa
      241 atatcttcct aacacccgcc ttgtgcgcat gatgcctaac accccttcga tcatcggccg
      301 cggtgtgatt ggctatttac tcaatgagat cgatgcggcc cttgaagctg tggtcgatga
      361 tatgtgttct cccttgggat atactctcaa ggttgaagat gaagaccaat ttgaagcctt
      421 gatgatctct tgctcttcag gaaccggctt tgttttagaa atgatgatgt attggcaaga
      481 ctggatcgtc gaacatggct ttgatgagga gacggcaaaa cgcatgactc tagagacctt
      541 tatgggggcc tcaatgttgg cggcccaatc caaagatatt ccttttgaag agctgcaagc
      601 tcgagttact tctaaaaaag gtgtcaccgc agctggactt cagtccatga gggaactcga
      661 aattgagcgc gcattgcgga ttagttttga aaaagcggct atgcgaaata aagaaatctc
      721 tcgcgaaacc aagtaagacc attgtaccct atggttggaa ttttaatgtc agtttcacgg
      781 aggaactccc aatgaaaatt acccctatcg atatagttca caagtctttc aataaaagaa
      841 tgctaggtct tgatgccgat gaagtcagtg acttcatggc gcagattgct tcgcaaatgg
      901 aagctcttat tcaagaaaga aataccttga aagaggctct tcgcgaaaaa gaactcgcgc
      961 tgctggatta taaagatcgt gaccaagtat taaaaagcac gatcgcgacg gcttcccaga
     1021 tgacagaacg ccttcgtcaa gactctgaga gagaggcgaa attaatcatc gccgacgcga
     1081 atcaaaagtc agagatgatc atccgggact cccgcgactc cttgaaaaaa atgtatcaag
     1141 aaattaccga tttaaaacgg gctcgcatgc agtttgaggc taatttaaaa gcactggtcc
     1201 aggcccatct aactttgctt gagcaaggtg aaaagtacat gccgcaaatg caattgccga
     1261 attacaatat cacgccttca ccgggaaaaa ccactgaggt ttctcccctt tctgcaggct
     1321 gaaatgttca ctcagacgga aaggggcatt aagctcgccc tttttattca acctaaagca
     1381 tctaaaaacg aaatcatagg gcctcacaat ggggccctca aaattaagat caccgcccca
     1441 cctgtggatg gcaaagccaa tgtggccctc atcgaatttc tttctgacgt tctcgatatt
     1501 cccaagcgtc aaattgaaat tctgaagggc gaaacgggtc gcaataaatc ggtggaaatt
     1561 ttcggcctca gccaggaaaa aattgaacaa aggctcggac tttccgggct gaattaatat
     1621 atcatgtctc gcccatccac gatggctatg aggttttcgg gtagaaacgc ctgaacgagt
     1681 gccgtgtcct ggatatagcc cactgaattt ttgctatcca tgagatcata attaatattg
     1741 ataatggcca ctttcaaaaa gcccttctct ttgccgagaa ccatgatcgg agttccggga
     1801 cgtaaagttc ctggttcacc tgtaatttgg ttttccttca agagatcttc ttgggcatcg
     1861 ggacctttat aaaaacggag tccttccgag gcgaggactt gaaaatccat ggctttgtcc
     1921 acattcttga aaaagacatc gagatgtttc aaagcgttct tttttccctc aggatcggca
     1981 tttgattttt cgaccgctaa gtaaagagcc gattttttcg atttgaaaga attgatcgct
     2041 tcagtcagtt gggcctgttg ccaccttccc cagctttgta aatggcctgg aaatccgcct
     2101 tcagatccag ctccaaaatt tcactatctt ggaaa
//
LOCUS       DATBTE010000253         6167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2014343, whole genome shotgun sequence.
ACCESSION   DATBTE010000253 DATBTE010000000
VERSION     DATBTE010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2014343"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VIG33_09265"
     CDS             <1..759
                     /locus_tag="VIG33_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEY8271066.1"
                     /translation="RHKITALEVAPTKYWSDLKSVSELSILHKKNQIEKLGLEIIAAQ
                     SLLFGRAELSVFGETAEVQKTITYLKRVIDVCALLGCKNVVFGSPKNRLKGPRSFAEA
                     CHEVAPVFIELAQHAEHRGLCFCIEPNAAAYGCDFVTTLTEALFLIEKVKHSHFGLHL
                     DIGNIIMAGENLEDSLRLGLGHAKHLHISAPQLVPIYHLDQSVSDEAWQLLTQYPLCL
                     SVEMLIKGSPEQIPAELNKSLQFLLRKLRGTSSQ"
     gene            806..3064
                     /locus_tag="VIG33_09270"
     CDS             806..3064
                     /locus_tag="VIG33_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271067.1"
                     /translation="MYLLYSFVALTLVYLCGDLLIPHLNYLKWDNFEFVIPAIIDAHS
                     LWLEGVVPVWSKFIHLGDPLIGNGQLGVFYFPHTLTLWTIRLFQLPYSVFPFASLLLH
                     SMISFIGWYLILRQWKLGKTISAVAGLSGVFGGMIQFNSQIWTDMLPCWAWFGILILG
                     IERRSLSLVALGISMGGMAAHPQQFFYLVLYGGIWSVVSLLLSKQIGKQNTKEKISEF
                     KEVLWGAIIGLLISLPSILPIFAQKSYSTRAASISLEEFLSRGVQWKDLLGMINPFLD
                     GTASVMPIQSSILLFQSYWVLPGILLGLLGFYKLKKTELQQKFLLTILVGAIALILCM
                     GGNTPLYVFTYKIPLWSSFRWPFKMLYFCLPLLIAAGALGWEMATSMTTQQLRTVFTF
                     SLLLFALSFLSSNIFENHLNPTGIFFLLSSFVLTVALALAYFFNKNRLKNSLWWGAVI
                     SSSLGILCLAQTNHNKDYTETYASMGKVELGLTTTDRVLPLSTLSEPFRQQSLGLMHS
                     GPMNNYESAGGCVTALAPIWRLKALPSDVYGVPARPYAFALLKSNLISTMNVGYLIVD
                     KKDLEMTTQLSRLSEYSLNSQTKDSLIFKNQKVLGKSFFAKKSFPATDANYENYVMGQ
                     AGTPDSVVVTPLNEEKSFTAQTQIERFSHVGGSIEIQLRPLDQTPQLLVVSSTWYPSW
                     SVYVDQKKQPVLQVNGTLMGVEVPAGARDIHFKWEDPMLNLGFIGFAIGILLWVCLAL
                     LTKYKFKLRRKT"
     gene            complement(3042..3809)
                     /locus_tag="VIG33_09275"
     CDS             complement(3042..3809)
                     /locus_tag="VIG33_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271068.1"
                     /translation="MSSALVGYTGFVGGNILKGYKFDHLYNSVNFSEIQGMKFDLLVF
                     SAASAAKWMANADPEKDKQHIDSLIKTLKTVSADRFVLISTIDVYKNPRNVTEDSETL
                     FSENHPYGLNRGRLEEFVKTNFSKSHILRLPGLYGPGIKKNVIFDFLNNNDIEKIDSR
                     GSFQFYNLNYITRDIQRVIEQDLSLINIAVEPVLVSEVYELYFGKPFVNKISDKPAVY
                     DFRSNFAALWGAPKEGYLYSRRECLEDLRKFMFSYGV"
     gene            complement(3802..4596)
                     /locus_tag="VIG33_09280"
     CDS             complement(3802..4596)
                     /locus_tag="VIG33_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8271069.1"
                     /translation="MREVPRYDRFCYRGKSSSYCVLIPVLNEAGKIEKQLQAMQEIAE
                     RFDVIIADGGSSDGSLTDDFLSSVNVRAKLVKTGPGKLSAQMRMALDYAMEEAYLGFI
                     FMDGNNKDNPEAIARFASKLSEGYDHVQGSRFIEGGKHENTPLSRHIAIRALHAPLIS
                     LAARFKYTDTTNGFRAYSRKFISDSRVQPFREIFSKYELHYYLAIRAARLGFRVIEIP
                     VERIYPASGKVPTKITGLRGYGLVMKTLLQACLGVFNPKKKEENHV"
     gene            complement(4607..5596)
                     /locus_tag="VIG33_09285"
     CDS             complement(4607..5596)
                     /locus_tag="VIG33_09285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8271070.1"
                     /translation="MIKNKDFLVSVVSPVKEINEVTLRYLENLLQVLKTSYEQYEVVL
                     VQDGISVKGEPSLISFVRGHEGCRVIKLMKTYGLDVAITAGLESAIGDVVVVCLPPFD
                     PTDKIVEFINVARAGYIGLGQRPPGHYEGMWHSFAKKVFYVVVNRFLNFGLPSDTTYF
                     VSLPRQAVTHIQRAKDRFRFLKVVTAQTGLPIQLLNYVPAEKDGRVYRTFWESILLSI
                     DVVTSQSLTLLRFASLLSLFVSLGSLSYLIYAFNIFLFKSEVQKGWSSLSIFISACFF
                     LLGLVFAILTEYLAKLFSESSNRPIYTIGNETVSDRVVANSHRANVLDTSKVD"
     gene            complement(5593..>6167)
                     /locus_tag="VIG33_09290"
     CDS             complement(5593..>6167)
                     /locus_tag="VIG33_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271071.1"
                     /translation="TSFNTKEFEVMAKEVFLVTYSKINETLAHSHLPIIPLRHEFTEM
                     ALVSVPEELQGLAVTVMDGPFWSMMPFPCRGLASLSHVRHTPHYQWSDEETFNPTANG
                     ELIKKTAFNFMIKDAARFVPLMSQVKHEDSLWEVKTILPRSDENDSRPILFQRDCGLK
                     GLHIVLGGKIDNIFDIISLIKQNPMKQDRK"
BASE COUNT         1684 a         1482 c         1263 g         1738 t
ORIGIN      
        1 cgccataaaa taaccgctct ggaagtggct ccgacgaaat actggtcaga tttaaagtct
       61 gtttcagaac tctctattct ccacaaaaaa aaccaaattg aaaaactggg gctagaaatc
      121 atcgcggctc agtctctttt atttggtcgg gctgaactca gtgtcttcgg ggaaacggcg
      181 gaagttcaaa aaaccatcac ttacttaaaa cgagtgatcg atgtctgtgc tttattgggt
      241 tgtaaaaatg tagtcttcgg ctctcctaaa aatcgcctca agggcccgcg aagtttcgca
      301 gaggcctgtc acgaagtggc tcccgttttt attgagcttg cacaacacgc cgaacacaga
      361 ggactctgtt tttgcattga acccaacgcc gcagcctatg gctgtgattt tgtgacaacc
      421 ctgacggaag ctctgtttct tattgaaaag gtcaagcatt ctcactttgg tttacatctc
      481 gatatcggca atattattat ggccggcgaa aatctggaag actccctgcg gctaggactc
      541 ggtcacgcaa aacatttgca tatcagtgcg ccccaattgg tgcctatata tcatttagac
      601 caaagcgtat cggatgaagc ttggcagctt ctcactcagt accccctttg tctttcagtg
      661 gaaatgttga taaaaggttc tcccgagcag atccccgcag agctaaacaa gtctcttcag
      721 tttcttctac gaaaactgag aggaacctcc tctcagtgaa ctggtctcaa ggagctggct
      781 aaatactttt ttataagggc ttttaatgta tttattgtat tcatttgtcg ctttaacact
      841 tgtctatctc tgcggggatc tgcttattcc ccatttgaat tatttaaaat gggataattt
      901 tgaatttgtg atcccggcga tcattgacgc ccattcgctg tggctcgaag gggttgtgcc
      961 ggtgtggagc aaatttatcc acttagggga ccccctcatt ggcaatggcc aactgggcgt
     1021 attttacttt ccgcatactc tgaccttatg gacgatccgc ttgttccagc ttccttactc
     1081 ggtctttccc ttcgcttcac tgcttcttca ctcgatgatt tcctttatag gttggtatct
     1141 gatcctccga cagtggaagc tagggaaaac catttccgcc gtggcggggc tctctggggt
     1201 ctttggcgga atgatccagt tcaattcaca aatatggacg gatatgcttc cctgctgggc
     1261 ctggtttgga attcttattt tagggatcga gcgcagaagc ctgagccttg tggccctagg
     1321 catctccatg ggcggaatgg ccgcccatcc gcaacagttt ttttaccttg tattgtacgg
     1381 cggaatttgg agcgtggtat ccctgctctt atccaagcaa attggaaaac aaaacaccaa
     1441 agaaaaaata tcagagttta aagaagtcct ctggggcgca ataattggat tgcttatttc
     1501 cttgccttcc attcttccca tcttcgctca aaaaagttat tcgaccaggg cagccagtat
     1561 ttctctggaa gagtttcttt ctcgaggagt ccaatggaaa gatttgctgg ggatgataaa
     1621 tcctttcctc gatggaacgg cctcggtcat gcccattcaa agctccattc tgctttttca
     1681 aagttactgg gtactgcccg gaatcctcct aggacttctc ggtttttaca aattgaagaa
     1741 gacagagctt cagcaaaaat tcctcttaac catcctcgtc ggcgccatcg cacttatctt
     1801 atgtatgggt ggaaacaccc cgctttatgt cttcacttac aaaatccccc tctggtctag
     1861 ttttcgctgg ccttttaaaa tgctgtattt ctgcttaccc ctgctcatcg ctgccggcgc
     1921 cctaggctgg gaaatggcaa cctcaatgac gactcagcag ttgcggaccg tctttacgtt
     1981 ctctctcttg ttgttcgcgt tgagtttttt atcttcgaat attttcgaga accatttgaa
     2041 tcccacagga attttctttt tattaagttc cttcgttctc actgtagcct tggcacttgc
     2101 ttattttttt aataaaaacc gtttaaaaaa ttccttatgg tggggtgccg ttatatcttc
     2161 aagcctcgga attttatgcc tggcccaaac caaccataat aaggattata cagagaccta
     2221 tgccagtatg ggcaaagtgg agttgggctt aaccacgaca gatcgggttt taccgctgag
     2281 cacactctct gaacctttcc ggcagcaaag cttgggcttg atgcattcag gtcccatgaa
     2341 taattacgag tccgctggtg gctgtgtcac ggctttagcg ccaatatggc gactcaaggc
     2401 tcttccttcc gatgtctatg gcgttcctgc aagaccttat gcctttgctc ttttgaagtc
     2461 caatttgatc agtacgatga atgtgggata tcttatcgtc gataaaaaag atcttgaaat
     2521 gaccacccag ctatcccgtc tttctgagta ttccttaaat agccaaacaa aagattcgct
     2581 catttttaaa aatcaaaaag ttctcggcaa aagtttcttt gcaaaaaaat cctttcccgc
     2641 aactgatgcc aactatgaaa attatgtaat gggtcaagcc ggcacccccg actctgtggt
     2701 cgtcacccct ctcaatgaag aaaagagttt cacagcgcag actcaaatcg aaagattttc
     2761 ccatgtggga gggtctattg agattcagct aaggccattg gatcaaactc cccaactctt
     2821 ggttgtgagt tcgacgtggt acccatcgtg gtccgtctac gtggatcaaa aaaagcaacc
     2881 cgtcttgcaa gtgaacggaa ccttgatggg agtggaagtt ccagcagggg ccagggatat
     2941 tcacttcaaa tgggaagatc ccatgctcaa ccttggattt atcggctttg ccataggaat
     3001 tctgttatgg gtttgtcttg cactgcttac gaaatataaa tttaaactcc gtaggaaaac
     3061 ataaactttc gtaaatcttc caggcattcc ctacgactgt acaaatatcc ctctttaggg
     3121 gcgccccata aggccgcaaa attcgatcta aaatcataga ccgcgggctt gtctgaaatt
     3181 ttatttacaa atggctttcc aaaataaagt tcgtaaactt ctgaaacaag caccggttcc
     3241 accgctatat ttatgagtga caggtcctgc tcgatcaccc tttggatatc ccgagtgatg
     3301 taatttaaat tataaaattg aaacgaaccg cgtgaatcaa ttttttcaat gtcgttgtta
     3361 tttaagaaat caaaaattac atttttttta atccccggac catagagtcc gggaagacgc
     3421 agaatatggg acttgctaaa gtttgtcttc acaaactcct ctaaacgtcc cctattcaag
     3481 ccgtaaggat gattttccga aaacaatgtc tcactgtcct ctgtgacatt tcgcggattt
     3541 ttatagacat cgatagtgga aataagcaca aatcgatctg ccgagaccgt ctttaacgtc
     3601 tttattaaag aatcaatatg ctgtttatct ttctccggat cggcgttggc catccatttg
     3661 gcggcgctgg cagcggaaaa gaccaggaga tcgaatttca ttccctgaat ctctgaaaaa
     3721 ttaactgaat tatacaggtg atcaaattta tatcccttca agatattacc acccacaaag
     3781 cccgtatatc caaccaaggc actagacatg attctcttcc ttcttcttag gattaaatac
     3841 ccccaagcag gcttgaagca aagtcttcat aaccaaccca tatcctctca agccggtaat
     3901 tttagtggga acttttcccg acgccgggta gatgcgctcc accggaattt caatcacccg
     3961 aaaccccaat ctcgcggctc gaatggcgag ataatagtga agttcatatt tgctaaaaat
     4021 ttcccgaaaa ggttggaccc gtgaatcaga aatgaatttc cttgaataag ccctgaatcc
     4081 attggtcgta tccgtatact taaatcgggc cgccaaggag atcagagggg catgaagtgc
     4141 ccgaatagca atgtgacgac tcaagggagt gttttcatgc ttccccccct caatgaatct
     4201 tgatccctga acatggtcat acccctcgct caatttgctt gcaaaacgag cgatggcctc
     4261 gggattgtct ttattattcc catccataaa aataaatccg agataagcct cttccatcgc
     4321 ataatccaaa gccatgcgca tttgcgcgct caacttccca ggtccggtct tcaccaattt
     4381 ggctcgaaca ttcaccgaac ttaaaaaatc atccgtcaaa gaaccgtcag agcttccccc
     4441 gtcagcaata atcacatcga aacgttcagc gatttcttgc atagcttgaa gctgcttttc
     4501 aatttttcca gcttcattga ggacaggaat taaaacgcaa taagaagaag atttaccacg
     4561 atagcaaaaa cggtcatacc taggaacttc tctcatttga accttcctaa tccactttcg
     4621 aagtgtctaa aacattcgcc ctatgggaat tagccaccac ccgatcggag accgtctcat
     4681 tgccgatggt ataaatcggt cgattgcttg attcggaaaa taactttgcc agatattccg
     4741 ttaaaatcgc gaacacaagg cctagcaaga aaaagcaagc ggaaataaaa atagagagcg
     4801 agctccatcc cttttgcact tctgacttaa aaaggaagat gttgaaagca tagatcaagt
     4861 aagacagaga tcctaaagaa acaaacaagg ataacaaaga ggcgaagcgc aacagagtca
     4921 gactttgcga tgtcactaca tcaatgctaa gaaggatcga ctcccaaaag gtcctataga
     4981 cccgcccatc tttttctgcg ggaacataat tcaaaagttg gatcggcaaa cccgtttgcg
     5041 ccgtgaccac tttcagaaat ctaaagcgat cttttgccct ttggatatgc gtcaccgctt
     5101 gccgaggaag actgacaaaa taagtggtat cggaaggcag cccgaagttc aaaaagcgat
     5161 tcaccaccac atagaatact ttttttgcaa aggagtgcca cattccttca tagtgtcctg
     5221 gcgggcgttg ccctaggcca atgtatccgg ctctagcgac attgataaac tccacgatct
     5281 tatccgtggg atcaaaagga ggtaagcaca ccaccaccac atcaccgatg gcactctcta
     5341 aaccagcagt gatggcgaca tccaaaccat aggtcttcat taattttata acccgacatc
     5401 cttcgtgtcc tctcacgaaa gagatcagcg atggttcccc cttcactgaa attccgtctt
     5461 gaacgaggac cacttcgtat tgctcatagg aggtctttaa cacttgcagc aggttctcaa
     5521 gataacgaag cgtgacttca tttatttctt tgaccggaga caccactgac accaaaaaat
     5581 ctttattttt tatcatttcc gatcctgttt cattgggttc tgctttataa gtgaaattat
     5641 atcaaagatg ttgtcgattt tccccccaag cacaatatga agtcctttaa ggccgcaatc
     5701 tctttgaaac aaaataggtc ttgaatcatt ctcatcgctt ctgggtaaaa tcgttttcac
     5761 ttcccacaga gagtcctcat gttttacctg actcatcagg ggcacaaatc gggcggcgtc
     5821 ttttatcata aaattaaaag ccgtcttttt tatcaactcc ccattggcag ttggattaaa
     5881 ggtctcctca tccgaccatt gatagtgcgg tgtatgtctg acatgactta aagaggccaa
     5941 tccccgacag ggaaagggca tcatcgacca aaaggggcca tccatgacgg tcacagccag
     6001 accctgtaat tcctccggca cagagaccag ggccatctcg gtgaattcat ggcgaagtgg
     6061 aatgatgggc agatgagaat gagccaaggt ttcattgatt ttagaatagg taactaaaaa
     6121 aacttccttg gccatgactt caaactcttt tgtattgaag gaagttc
//
LOCUS       DATBTE010000254        13953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2288324, whole genome shotgun sequence.
ACCESSION   DATBTE010000254 DATBTE010000000
VERSION     DATBTE010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 13953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13953
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2288324"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VIG33_09295"
     CDS             <1..476
                     /locus_tag="VIG33_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271072.1"
                     /translation="EAFDENQMTFALAAETLRILQWNPINSVGKKVNLERSLRFGDRI
                     HGHLVTGHVDSLAEVVRADLEGESFFLNVKVASSLLPYIWKKGGLTINGVSLTVNEIK
                     NSVVEVCLIPETLKRTNLGNLKAGQVVNIEPDYLARALVAVVDAKNTVSKNMECQ"
     gene            473..1114
                     /gene="ribB"
                     /locus_tag="VIG33_09300"
     CDS             473..1114
                     /gene="ribB"
                     /locus_tag="VIG33_09300"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEY8271073.1"
                     /translation="MSLNSIEELVSEIRAGKMVILIDDEDRENEGDLVLAADHVTPEA
                     INFMAREARGLICLPLSSQQIERLQLPQMVPVELNHSSNKTAFTVSIEAASGVSTGIS
                     AADRAHTIKVAANPLARPTDVHMPGHIFPIRAQQGGVLNRAGHTEGSVDLATLAGLNP
                     AAVICEVINDDGTMARLGDLKLFAKKHNLKIGTIVDLIQYRKKIKEENETANT"
     gene            1095..1592
                     /gene="ribH"
                     /locus_tag="VIG33_09305"
     CDS             1095..1592
                     /gene="ribH"
                     /locus_tag="VIG33_09305"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY8271074.1"
                     /translation="MKPQIPKVGVVTARFNSEVTDKLEEGALSYLENCGIEVLAVKVP
                     GAVEIPLACKALLDIGCEGVVALGAVIRGETSHYDYVCNSVERGVTTLMLEYKKPIGF
                     GVLTTENDEQALERAGGKHGNKGTECAQVVLEMMGLLSELAKEGQKPIRKKTAKKSTK
                     KVKKK"
     gene            1602..3893
                     /locus_tag="VIG33_09310"
     CDS             1602..3893
                     /locus_tag="VIG33_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8271075.1"
                     /translation="MTQSKKPVEADSEIREEKFVDLSPKMEQRKRRREIFTVAVLSLL
                     VFFLTWFEIRILATSKTLPFVHSIFFFGLVNFNIILLLLLLFLIFRNIVKVFVERRGK
                     IFGSSLKAKLIAAFVAFSTVPTLLMFIISVFYINSSFDKWFSVKMVGVLKSSLEVTNA
                     YYAGTKMKNYHFAHQIAAKVQTERNPQIIQKNLNQLRKEYALDSVEYYPSIFQGRLMA
                     FAEDDNIPPVPPVSLEFLQKGVKSHKEWSTIHQFGEGNLVRVIVPVKEGEDRGAVVVS
                     SFVPLSLISRMNDVGTAYEEFREINPLEYPLKSIYLIILFLITFVILLAATWFGFHLA
                     KQLAVPLVQLGKATLRVAGGDYSQKLATSSGSEEIALLITSFNKMTETLEASEREVKE
                     ANLTLQHTLQDLAQHNRYIEVVLGNVSTGVISVGKNGHITTLNRHAGDLLKIDPAKYI
                     GRPVRELLTLEYFRTFSELLKTMQDHEIEHIQKEFHVNVQGQSIPLQMNLSILKDEKG
                     DEVGKILVFDDMSPIVNAQRAAAWTEVARRIAHEIKNPLTPIKLSAERLQRKFGAQLT
                     DPAFKECTSMIIHQVDDLKKMVNEFSQFARLPQSKPVAGTLNKVVEESLMVFRQAHPQ
                     VKFEFRDDVNMPDFKFDPDQIRRVFVNLIDNAVAAIGKESKPEIKVTTFYDADLKIAR
                     LTVADNGEGIPVSDRNRIFEPYYSTKEGGTGLGLPIVKRIIEDHNGFIRATANDPKGT
                     KMLMELPVNAVDAWTPSKAGSKV"
     gene            3890..5284
                     /locus_tag="VIG33_09315"
     CDS             3890..5284
                     /locus_tag="VIG33_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8271076.1"
                     /translation="MSSNIISSSSENKNYKILIIDDEASIREVLGATLKDEGYTVSSA
                     HDGVSGLQAIRQFHPDIVFLDIWMPGELDGIQVLNQARKEFPAVDFVMISGHGTIETA
                     VKATKLGAYDFIEKPVSMDKIIIIISNILNYQQEKEEKILLLNKLRKSIALIGEAPAI
                     MATKQIIAKMAPTHSWILVTGETGVGKALAAQNIHYMSTRAGKAFVDVNCGAIPEDLL
                     ESEIFGIEKGAMPGVDKVKKGKLELANGGTLFLNEVSALTPEVQHRLVKFYETKSFSR
                     VGGSDGIHNDVRIIATTTKDLEKMVKDGSFSEALYYKLNIIPFRMPTLRERPEDIAVL
                     TSYFSDHVAREGGYLKKAFSEQATEAMMKYEWPGNVRELKNFIERVYILTPGEFVDLH
                     DVRFAGLIGSEDAKMSLEAMSTFREARAQFEKEYLIKKIAENGGNISKTAEVIGLERS
                     YLHRKIKSYGIEVL"
     gene            5284..6786
                     /gene="proS"
                     /locus_tag="VIG33_09320"
     CDS             5284..6786
                     /gene="proS"
                     /locus_tag="VIG33_09320"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY8271077.1"
                     /translation="MANTAITPTRSENYPEWYQEVIKAADMAENSPVRGCMVIKPWGY
                     ALWENMQKILDGKFKETGHVNAYFPLLIPLSFLEQEAEHVEGFAKECAVVTHHRLKAG
                     PDGKLIPDGKLEEPLIIRPTSETIIGHMYAKWVKSYRDLPLLMNQWCNVMRWEMRTRM
                     FLRTTEFLWQEGHTVHATAQEAQEETLRMLDVYADFAENYMAMPVIKGMKTPDERFPG
                     AVDTYTIEALMQDRKALQAGTSHFLGQNFAKASGIKFLSAEGKEEVAWTTSWGVSTRL
                     IGGLIMSHSDDDGLVLPPKIAPIQVVILPIYRNADERSQVLEYVKSLEKEIRATKYAD
                     QSVSVKIDDRDLRGGEKAWQYIKQGVPVRLEVGPRDMAKGEVFVGRRDKGVKEKSGMA
                     RTSFVESLPQILEEIQQNIYNRAKSSRDANTKKIENIQDFENFFGGDEESQTGGFALV
                     HWNEAAIGHELLAKLKVTPRCIPLEGPEENGTCIFTGKPSKRRVIFAKAY"
     gene            6914..7324
                     /gene="arfB"
                     /locus_tag="VIG33_09325"
     CDS             6914..7324
                     /gene="arfB"
                     /locus_tag="VIG33_09325"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEY8271078.1"
                     /translation="MNNELLQQIISREVSFEFTRSRGAGGQHVNRTESAVILRWNLID
                     TQAFIGSVKERLVNKLSTQLTNEGELLIRAETHRDQDSNRKEAIRRFEEILDKALFVP
                     KKRIKTKPTKSSQRKRLDSKKKDSNNKRLRSEKW"
     gene            7341..8180
                     /locus_tag="VIG33_09330"
     CDS             7341..8180
                     /locus_tag="VIG33_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8271079.1"
                     /translation="MTKNISESTDFYAQNFIHLKLQKKSDQLWITLDNPEQGNAITPG
                     MIESLCKVLKYADFDPKIRVFIITGAGKNFCSGGDVKAMSERTGMFQGESNELRLRYM
                     HGIQQIPKTIEDLSTPLIAMVNGAAIGAGCDLAMMCDLRVGASTTKFGETFTKIGLVP
                     GDGGTFFLQRIVGYAKAMEMTLTADVYEGQKAKDFGLMNFLVEDAHLLMETEKLAEKI
                     ANNAPVAQQMAKKAMKISYLHDLQTSLDLLASFQGIAQRTQDHFEALEAVKGQRPPHF
                     QGQ"
     gene            8199..8918
                     /gene="pyrF"
                     /locus_tag="VIG33_09335"
     CDS             8199..8918
                     /gene="pyrF"
                     /locus_tag="VIG33_09335"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY8271080.1"
                     /translation="MKPDQRSHPMRNPIIAALDVDDITHALKLADELADIVGGFKVGP
                     RLCLRYGQELVKEIAARGPVFMDNKHFDIPSTMEAAVRASFEAGATLVTVHALSGYEA
                     LVKMAKLEKELTLIRPFKILAVTILTSWDQKSLPGNLKQQPISQHVVELADLVLSSGM
                     TGLVCSPHELDLLQNKGLYLVTPGIRFNMEGLGDQKRVMGPDEAIKAGATALVVGRPI
                     IEAKKPRETATDYVMALYEKK"
     gene            8905..9759
                     /gene="rlmB"
                     /locus_tag="VIG33_09340"
     CDS             8905..9759
                     /gene="rlmB"
                     /locus_tag="VIG33_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEY8271081.1"
                     /translation="MKKNNSRFGNKSQPAKTPSRRPSPTQLGAPRSRENEIPKEWRPV
                     IGTHAIKEALSVHASKGKLLWLRQSWESSVDLREIHEIAQAARVKIELKPDSVVDRFG
                     TSHQGAVLFMNGAPTLSMEELARLDKSITLILDGIEDPHNLGAILRTSWLVGVQGVLV
                     PENRAVSLTPTVHKVACGGVEHVPVVETTNFTKYVEDFKKMGYWVYGLSPRGKKSIFE
                     LNLPHKVVWAIGSEEKGMRVTTERLCDELVYIPQSSAAASYNASVATAMALTETLRQH
                     VNSGPIKK"
     gene            9860..9935
                     /locus_tag="VIG33_09345"
     tRNA            9860..9935
                     /locus_tag="VIG33_09345"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9893..9895,aa:Thr,seq:tgt)
     gene            9965..10050
                     /locus_tag="VIG33_09350"
     tRNA            9965..10050
                     /locus_tag="VIG33_09350"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9999..10001,aa:Tyr,seq:gta)
     gene            10141..10217
                     /locus_tag="VIG33_09355"
     tRNA            10141..10217
                     /locus_tag="VIG33_09355"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10175..10177,aa:Gly,seq:tcc)
     gene            10227..10301
                     /locus_tag="VIG33_09360"
     tRNA            10227..10301
                     /locus_tag="VIG33_09360"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10259..10261,aa:Thr,seq:ggt)
     gene            10398..11591
                     /gene="tuf"
                     /locus_tag="VIG33_09365"
     CDS             10398..11591
                     /gene="tuf"
                     /locus_tag="VIG33_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEY8271082.1"
                     /translation="MSKEKFTRTKPHVNIGTIGHVDHGKTTLTAAITTTLATTGQAQA
                     MSYDQIDKSPEERARGITISTTHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSSADGPMPQTREHILLARQVGVPALVVFMNKVDMVDDKELLELVELEVRELLSK
                     YEFPGDDIPVVKGSALKALEGDTSEIGRPSILRLMEACDNYIPTPARAVDKTFLMPIE
                     DVFSISGRGTVVTGRVERGIVKVGEEVEIVGIRPTQKTTVTGIEMFRKLLDEGQAGDN
                     CGVLLRGTKKEDVERGQVLAKPGTIKPHKKFKGEAYILTKEEGGRHTPFFNGYRPQFY
                     FRTTDVTGVCTLKAGTEMVMPGDRVEISVELIAPIAMEKELRFAIREGGRTVGAGVVT
                     EILAD"
     gene            11686..11761
                     /locus_tag="VIG33_09370"
     tRNA            11686..11761
                     /locus_tag="VIG33_09370"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11719..11721,aa:Trp,seq:cca)
     gene            11806..12183
                     /gene="secE"
                     /locus_tag="VIG33_09375"
     CDS             11806..12183
                     /gene="secE"
                     /locus_tag="VIG33_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEY8271083.1"
                     /translation="MEKTNSKIITLSFAIGAFLTGLVVSLLIKAFAGAFAIVARAADS
                     DMFRHGVPLMIGFALFAYLQFNSKIVTWADEVAVEVRKVVWPSRKDTVAMTIVVIVMV
                     LISSLIISTFDLVSANIINTLMR"
     gene            12193..12726
                     /gene="nusG"
                     /locus_tag="VIG33_09380"
     CDS             12193..12726
                     /gene="nusG"
                     /locus_tag="VIG33_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEY8271084.1"
                     /translation="MDKKWYIVNTQTGCESTAKTAIEERVHSQKMEEFFGEILIPSEN
                     VVELVKGQKQTKSRKFFPGYIFVQMFLNDETWHLVKHSSKVTGFVGGAKTRPPEVPEA
                     EVLRVTQQMAGVAEKPKQKVKFSVGENVTVVDGPFSNFNGTVEDINEDKAKVKVLVSI
                     FGRPTPVELDFVQVEKN"
     gene            12781..13206
                     /gene="rplK"
                     /locus_tag="VIG33_09385"
     CDS             12781..13206
                     /gene="rplK"
                     /locus_tag="VIG33_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEY8271085.1"
                     /translation="MAKKVTGMIKLQIPAGKANPAPPVGPALGQHGVNIMEFCKQFNA
                     RTQALGDSIIPIIITVYQDRSFTFITKTPPVSSLLKKAVKLESGSKMPQKDKVGKVKL
                     EQVKQIATTKLPDLNCLKVESAMSQVIGTAKSMGLELAD"
     gene            13219..13917
                     /gene="rplA"
                     /locus_tag="VIG33_09390"
     CDS             13219..13917
                     /gene="rplA"
                     /locus_tag="VIG33_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEY8271086.1"
                     /translation="MANKRFKAASKKVDSTKKYTVDEAFKMVVESATAKFDETIDVAL
                     RLGIDPKQSDQQVRGAIALPHGLGKNVKVVVFAKGPKETEAKNAGADFVGADDLVAKI
                     QGGWLDFDKCIATPDMMATVSKVAKILGPRGLMPNPKVGTVTMNVGDAVTAEKKGKLD
                     FRVDKAGIVHAGVGKKSMGDTKLRENFLALLGAIVKAKPAASKGIYLRTISVASTMGP
                     GLKIDPNQASGVVI"
BASE COUNT         3984 a         2879 c         3366 g         3724 t
ORIGIN      
        1 tggaagcttt cgatgaaaac caaatgacct ttgccttggc tgcggaaact cttcgaatcc
       61 tccagtggaa tccaataaat tcggtaggca aaaaagtgaa tctagaaaga tcccttcgtt
      121 ttggggatcg tattcatggt catcttgtta ctggtcatgt cgacagcctt gccgaggttg
      181 tgcgtgccga tctagaggga gaatcttttt ttcttaatgt caaagtggcc tcgtcacttc
      241 tgccttatat ttggaaaaag ggaggcctca ccattaacgg cgtgagcctc accgtgaatg
      301 aaattaaaaa ctcagtggtt gaagtttgtc tgattcccga gactttaaag cgtacaaatc
      361 taggaaattt aaaagcaggt caagttgtaa acattgaacc agattatctg gctcgtgcgc
      421 tggtcgcggt tgtagatgca aaaaatacag tttcaaaaaa tatggagtgc caatgagttt
      481 aaattctatt gaagagctgg tttcagaaat tcgcgccggt aaaatggtta ttctcattga
      541 tgatgaggat cgagagaatg agggtgattt agtgctagca gccgatcacg tgacccccga
      601 agccattaac tttatggcac gtgaggctcg aggtttaatt tgtttaccgc tcagctctca
      661 gcagatagaa cgtttgcaat tgccacaaat ggttccggtg gagttgaacc attcttcaaa
      721 taaaacagct tttactgtca gtattgaagc cgccagtggt gtgagtaccg gcatttccgc
      781 ggcggatcga gcccatacta tcaaggtggc tgcgaatcca cttgcaaggc ccacggatgt
      841 tcatatgccc ggccatattt ttccgatccg tgctcagcag ggtggagtcc tcaatcgtgc
      901 cggccatacc gagggtagtg tggatctcgc cactttagcg gggcttaatc ctgcggcagt
      961 gatctgtgaa gtgatcaatg acgatggaac tatggcccga ctcggagatc tgaagctttt
     1021 tgcaaaaaaa cataatttaa aaattggaac cattgtcgat cttatccagt atcgaaaaaa
     1081 aataaaggaa gaaaatgaaa ccgcaaatac ctaaagtcgg agtggtcact gcgagattta
     1141 atagcgaagt gactgataaa ttggaagaag gcgctctgag ttatctcgaa aactgtggta
     1201 ttgaagttct tgcggtaaaa gtcccaggag ccgtcgagat tccgctcgcc tgtaaagctc
     1261 tgttagacat cggctgcgag ggagttgttg ccctgggtgc ggtgattcgc ggagaaacct
     1321 cccattatga ttacgtttgc aatagtgttg aacgaggtgt gacgacctta atgcttgaat
     1381 acaagaaacc aattggtttt ggagttctca ccacggaaaa cgacgaacaa gctctagagc
     1441 gcgccggtgg taaacatggc aataaaggaa ctgaatgcgc tcaggttgtt ctcgagatga
     1501 tggggcttct cagtgagctg gcgaaagagg gccaaaagcc aattcgtaaa aagactgcaa
     1561 aaaaatctac caagaaagta aaaaagaaat aaacttagaa aatgactcag agtaaaaaac
     1621 ctgtagaagc cgatagtgag atccgcgaag aaaaattcgt cgatctttct ccgaaaatgg
     1681 agcagagaaa aaggcgtcgt gagattttca cggtggcggt cctatctttg ctcgttttct
     1741 ttttgacttg gtttgaaatc cggattctcg caaccagcaa aacattgcct tttgttcaca
     1801 gtatcttttt cttcggactc gtgaatttta atattatttt attgttgctt ctgctttttc
     1861 ttatcttccg taatatcgta aaggttttcg tcgagcgccg gggaaagatt tttggcagca
     1921 gcttaaaggc taagttgatc gctgcctttg tcgcttttag caccgtgccc accttgttaa
     1981 tgttcattat ctcggttttt tacattaatt ccagttttga caaatggttt agcgtaaaaa
     2041 tggtgggagt tttgaaaagt tctcttgaag tgaccaatgc ctactatgcg ggaactaaaa
     2101 tgaagaacta ccacttcgcg catcagatag ctgcaaaagt tcaaacggaa cgcaatcctc
     2161 agatcataca gaaaaacctc aaccaactcc gcaaagagta tgctctggac tcggtggaat
     2221 attatccatc gatattccaa ggacgcctca tggcttttgc agaggatgac aatatcccgc
     2281 cggttcctcc agtgtcctta gagttcctgc aaaaaggcgt caagtcccac aaagagtgga
     2341 gcaccatcca tcaattcggg gagggtaatc tagtccgggt gattgtaccg gtaaaagagg
     2401 gcgaagatcg cggtgctgtg gtcgtttcta gttttgttcc cttgtctttg atttctcgga
     2461 tgaacgatgt aggaaccgct tatgaagagt tccgagagat caatcccctg gaatatccac
     2521 ttaagtccat atacttgatt atcttatttt taatcacctt tgtgatttta ctcgcagcca
     2581 cctggttcgg gtttcatctg gctaaacaac ttgcggttcc cttggtgcaa ctcgggaaag
     2641 ccacactccg agtagccggt ggagattaca gccaaaaact agcaaccagc tctggaagtg
     2701 aagagatcgc cctattaatc acttcattta ataaaatgac ggagactttg gaagcctctg
     2761 aacgcgaagt gaaggaagca aatttaactt tgcagcacac tttgcaagat ttggcacaac
     2821 acaatcgata tatcgaagtc gtgctgggaa acgtaagtac aggcgtaatt tccgtgggta
     2881 agaacgggca tatcaccact ttgaatcgcc atgccggaga tcttttaaaa atcgatcccg
     2941 ccaagtatat cggtcgtccc gtacgcgaac ttttgacctt ggagtacttc cgcactttct
     3001 ctgagctttt aaaaaccatg caggatcatg agatcgagca tattcaaaag gaattccatg
     3061 ttaatgtgca aggacaatct ataccgctgc aaatgaacct ttcgatcttg aaggatgaaa
     3121 aaggggatga ggtcggtaag atcctggtct ttgatgatat gagtccgatc gtgaatgctc
     3181 agcgggcagc tgcctggacc gaagtggctc gccgtattgc ccacgagatt aaaaatccgc
     3241 tgactccgat caagctttct gcggaacgtt tgcaaagaaa atttggcgct caactgacag
     3301 atccagcctt taaagaatgt acgagtatga ttatccatca ggtggatgat cttaagaaaa
     3361 tggtgaatga gttcagtcaa ttcgctcgtt tgcctcaaag taaaccggtc gccggaactt
     3421 taaataaagt ggttgaagag tctctgatgg ttttcaggca agctcatcct caggtgaaat
     3481 ttgaatttag ggatgatgtc aacatgccgg attttaagtt tgatccggat caaatccgcc
     3541 gcgtttttgt aaatttaatc gataacgccg tggcggccat tggtaaagaa tctaagccag
     3601 agatcaaagt gacgactttc tatgatgcgg acttaaagat cgccagactc acagtggcag
     3661 ataacggtga aggaattcca gtcagtgacc gcaaccgtat ttttgagccc tactacagca
     3721 ccaaagaggg tggcaccggt ctaggtctgc caattgttaa acgaattatt gaagatcata
     3781 acggttttat tcgcgcaacg gcgaacgatc caaagggtac taaaatgctg atggaacttc
     3841 cagtgaacgc cgttgatgct tggaccccat cgaaagcagg gagcaaagtg tgagtagcaa
     3901 cattataagt agtagcagtg aaaacaagaa ctacaaaatt ttaattatcg acgatgaagc
     3961 ttcgatccgc gaagtgctgg gggccacttt aaaagatgaa ggctatacag tttctagcgc
     4021 ccatgatggg gtttcgggtc tccaagccat tcgccagttc catcctgata tcgtattttt
     4081 ggatatttgg atgcctggag agttggatgg gattcaagtg ctcaatcaag ctcgcaagga
     4141 gtttcctgcg gtagattttg tgatgatttc aggtcacgga acgattgaga ctgcggtgaa
     4201 ggcaaccaaa ctcggtgctt acgactttat cgaaaagccc gtttccatgg ataaaattat
     4261 catcattatt tccaatatct tgaactatca gcaggaaaaa gaagaaaaga ttcttctctt
     4321 gaataaactg cgtaagagta ttgcgttaat tggagaagct ccagcaatta tggcgacaaa
     4381 gcaaatcatc gccaagatgg ctcccactca ttcctggatt ttggtgactg gggaaaccgg
     4441 cgttggcaaa gctttggccg cccaaaacat ccattacatg agcactcggg ccggaaaggc
     4501 ttttgtggat gtgaactgcg gggctattcc ggaagattta cttgagagtg aaattttcgg
     4561 tattgaaaaa ggcgcgatgc caggtgttga taaggttaaa aaaggaaagc tcgagcttgc
     4621 taacggtgga actctttttt taaatgaagt ttcggcactg acaccagaag ttcagcatcg
     4681 gttagtgaag ttctatgaga ccaagtcttt ttcacgcgtc ggtggttcag atggaattca
     4741 caacgacgtt cgtatcattg cgacgaccac caaagatctt gaaaaaatgg taaaagacgg
     4801 ttcctttagt gaagcgcttt actataagct caatattatt ccattccgta tgccaacttt
     4861 gcgggaacgt ccggaagaca ttgccgtttt gacttcttac ttcagtgacc atgtggcgcg
     4921 cgaagggggc tatttgaaaa aggccttttc tgagcaagcc acggaagcca tgatgaaata
     4981 cgaatggcct ggcaatgtgc gtgagcttaa aaatttcatc gagcgagttt atattctgac
     5041 gcccggagaa ttcgtggatc ttcacgatgt gcgtttcgct ggacttatcg gctctgaaga
     5101 tgcgaaaatg agcctcgagg caatgtcaac tttccgagaa gcccgtgcgc aatttgaaaa
     5161 agaatacttg atcaaaaaaa ttgcggaaaa cggcggcaat ataagtaaga cggctgaagt
     5221 tattggtctt gaaagaagct acttgcatcg taagattaaa agctatggaa ttgaggtatt
     5281 gtaatggcaa atacggcaat cactcccact cgttcggaaa actatcctga atggtatcaa
     5341 gaggtcatca aagctgcgga catggcggag aattccccag ttcgtggatg catggttatt
     5401 aaaccctggg gctatgctct ctgggaaaat atgcagaaga ttctcgatgg aaagttcaaa
     5461 gagaccggcc atgtaaatgc ttactttcct ctccttattc cgttgagttt tcttgagcaa
     5521 gaagccgaac acgttgaagg ttttgccaaa gaatgcgcgg tggtaaccca tcatcgtctc
     5581 aaagcgggcc ctgacggtaa gctgattcct gacggcaaac tggaagagcc acttatcatt
     5641 cgtccaacga gtgaaacgat cattggccac atgtatgcga aatgggttaa gtcttatcgt
     5701 gatttgcctc ttttgatgaa tcagtggtgc aatgtcatgc gctgggaaat gcgcacgcgc
     5761 atgttcctcc ggaccacaga gtttttgtgg caagagggac atacggttca tgcaaccgct
     5821 caagaagcgc aagaggaaac tctgcgcatg ctcgatgtct atgcggattt cgctgaaaat
     5881 tatatggcga tgccagtgat caaaggcatg aaaactccgg atgaaagatt cccgggagcc
     5941 gtggatacct acaccataga agctttgatg caagatcgta aggctttgca agctggcact
     6001 tcgcattttc taggtcagaa ttttgcaaaa gcttcaggta ttaaatttct gagcgctgag
     6061 ggcaaagaag aagttgcttg gaccacctcc tggggagttt ccacgcgttt gattggtggc
     6121 ttaatcatga gccacagtga tgacgacggc ttggttcttc cgccaaagat tgcaccgata
     6181 caggtcgtaa ttttacccat ctatcgcaat gccgatgaac gctctcaagt tctggaatac
     6241 gtgaagagtc tagaaaaaga aattcgtgca accaaatacg cagaccaatc cgtcagcgta
     6301 aaaatcgatg accgcgatct gcgtggtggt gaaaaagcct ggcagtatat caaacaaggt
     6361 gttccagttc gcctcgaagt ggggccgcga gacatggcta agggcgaagt ctttgtaggt
     6421 cgtcgcgaca agggcgtaaa agaaaagtca ggaatggctc gcacttcctt cgtagagagc
     6481 ttgccacaaa tcctcgaaga gattcagcaa aacatctaca atcgtgcgaa atcgtcccgc
     6541 gacgccaaca ccaaaaaaat cgaaaatatt caagattttg aaaatttctt tggcggtgat
     6601 gaggagtccc aaaccggagg ttttgccctg gtccattgga acgaagcggc cattggccac
     6661 gaactcttgg cgaagctgaa agtaactcca cgctgcatcc ctctcgaggg accagaagaa
     6721 aacggaacct gcatcttcac cggcaaacct tccaagcgcc gcgtgatctt tgcgaaagcc
     6781 tattagtaat tcagcggagt atccaacaga gttggatact cccaccactc tcattttgat
     6841 atctgattaa agtctttgac ataataataa aaagagttga acgcccagtt ctctcacctg
     6901 taaaatagag ctcatgaata acgagctttt acagcaaata atctctcgag aagtgagttt
     6961 cgagtttacg cgaagccgtg gagccggagg tcagcatgtc aatcgtactg agtccgccgt
     7021 gattttgcgt tggaatctca tagatactca ggcttttatt ggcagcgtta aagaacgtct
     7081 ggtgaataag ctcagcactc aacttacgaa cgaaggtgag cttctgattc gggcggaaac
     7141 gcatcgcgat caagatagca atcgcaaaga agcgatccgc cgctttgaag aaattcttga
     7201 taaagccctc tttgtgccta agaagcgcat taaaactaaa cctactaaat cctctcagcg
     7261 caagcgttta gattccaaga aaaaagactc caataataaa cgcctgcgca gcgagaaatg
     7321 gtaagaaaac gaaatagagc atgactaaaa acatttcaga aagcacagat ttttacgcac
     7381 agaatttcat tcatctcaag cttcaaaaaa agagtgatca actgtggatc accttggata
     7441 atcctgaaca aggcaatgcg atcaccccgg gaatgataga atctctctgc aaagtcttaa
     7501 aatacgcaga cttcgatcct aagattcgag ttttcattat tacaggagct gggaaaaact
     7561 tttgttctgg gggagatgta aaggcaatgt ccgagaggac gggaatgttt cagggcgaaa
     7621 gcaatgagct tcgtctgcgt tacatgcatg gcatccagca gattccgaaa accatcgaag
     7681 acttatcgac tcctctcatc gccatggtta acggagccgc tatcggcgca ggctgtgatc
     7741 tcgccatgat gtgcgatctg cgtgttggag cctccacaac caagttcggc gagactttca
     7801 ccaaaatcgg tttagttccc ggtgacggcg gcacgttctt tttgcaacga attgtaggct
     7861 atgcaaaagc catggaaatg acattgactg ctgatgtgta tgaagggcaa aaggctaaag
     7921 actttggttt aatgaatttt cttgtcgaag atgctcatct tttaatggaa accgagaagc
     7981 tagcagaaaa aatcgcaaat aatgctcctg tggctcagca gatggcaaag aaagcgatga
     8041 aaatttctta tctgcatgat ctccaaacat ccctggattt gctggcaagc tttcaaggaa
     8101 ttgcgcaaag aacccaagat cacttcgaag ctctagaagc cgtgaagggt cagcgcccgc
     8161 cacattttca agggcagtaa aaaaaggaga agatttctat gaagcctgat cagcgttctc
     8221 accctatgag aaaccctatt attgcagcat tggatgtgga cgatataaca cacgccctca
     8281 agttggcgga tgaattagcg gatattgttg gcggattcaa agtggggccg cgcctttgcc
     8341 ttcgttacgg ccaggaactg gtgaaagaaa ttgccgcccg tggcccggtc tttatggata
     8401 ataaacactt cgatataccc tccaccatgg aggcggctgt tcgggccagt tttgaagcgg
     8461 gagcgacact ggttacagtg catgcccttt caggctatga ggctcttgtg aaaatggcta
     8521 agcttgaaaa agaactcact ctgattcgtc ccttcaaaat tcttgcagtg accattctca
     8581 cctcatggga tcagaagtcg ttaccaggaa atcttaaaca acaacctatt tctcagcatg
     8641 tggtggagct tgcggacctg gtcctttctt cgggaatgac gggcttggtt tgttcacccc
     8701 atgaactgga tcttttacaa aacaaaggcc tctatcttgt aactcctgga attcgattta
     8761 atatggaggg cctcggagat caaaagcgtg taatgggtcc tgatgaagcc ataaaggcgg
     8821 gagctactgc tcttgtggta gggcgaccga ttatcgaagc aaagaaacct cgggaaaccg
     8881 caacagatta tgtgatggca ctctatgaaa aaaaataact cacgatttgg caataaatcc
     8941 caacccgcaa agaccccttc aagacgtcct tcgccgactc aattaggcgc acctcgcagc
     9001 agggaaaatg aaattcctaa agaatggcgt ccagtgattg gcacccatgc gatcaaagaa
     9061 gccctcagcg ttcatgcctc taagggaaaa ctcctttggc tgcgacaaag ctgggaatct
     9121 tctgtcgatc ttcgggaaat tcatgagatc gctcaagcag ccagagttaa aattgagctt
     9181 aaacctgata gtgtggttga tcgtttcggg acttctcatc aaggagccgt gttatttatg
     9241 aacggcgcgc ccactttatc gatggaagag cttgctcgtc tggataaatc catcactctc
     9301 attttggatg gcatcgaaga tcctcataat ttgggagcaa ttcttcgtac ttcttggctc
     9361 gtgggagttc aaggcgtcct tgtacctgaa aatagagcag tcagcctcac gccgacagtc
     9421 cataaagtgg cttgcggagg agttgaacat gttcctgtcg tggagaccac aaattttact
     9481 aagtacgtag aagactttaa aaagatgggt tattgggtgt atggtttaag cccacgcggt
     9541 aagaaatcta ttttcgaatt aaacttgccc cataaagttg tttgggcgat tggttccgaa
     9601 gaaaaaggca tgcgagttac cacagaaaga ctttgtgatg agctagtata tataccgcag
     9661 agcagtgcag cagcgagtta caatgcttct gtggctacgg cgatggctct cacagagaca
     9721 cttcgtcagc atgtgaattc cggtccaata aaaaaataaa ttgaaatcga ataatactct
     9781 ttgactggca tgggtttttt tcagtacaaa gtttcggttg atagattcga catattaatt
     9841 attcgtattt atcgaaggtg ctggtttagc tcaattggta gagcagctga tttgtaatca
     9901 gcaggttgcg ggttcgagtc ccataaccag ctccaagttt tttggagtgc acgttcctaa
     9961 aaacggacag gtgcccgagt ggctaaaggg gacggactgt aaatccgttg gcttatgtct
    10021 acgaaggttc gaatccttcc ctgtccacca tctttagttg cgtttagtcc aaaagacttg
    10081 aaatgagtgt aaaggtgaaa accaaagcat ttagaccaag tttagtaagg atcgagtcga
    10141 gcgggagtag ctcaattggc tagagtatct gccttccaag cagagggttg cgggttcgag
    10201 acccgtctcc cgctccaaaa ctaagcgccc ttgtagctca gtggtagagc gcacccttgg
    10261 taagggtgag gtcgtcggtt cggctccgat caagggctcc atcaaaaatg atgactagat
    10321 tttgcgaatt gttttgaact ttatttgaat atgaatttga attttaaaaa ttaataagat
    10381 ctcttcggga ggaaataatg tctaaagaga agttcacacg tactaagccc catgtaaaca
    10441 tcggcactat cggtcacgtc gatcacggta aaactacttt gactgcggcg atcactacga
    10501 ctttggctac tactggtcaa gctcaagcta tgtcttatga tcaaatcgat aagtctccag
    10561 aagagagagc gcgtggtatc actatctcta ctactcacgt tgagtacgaa actgcaaatc
    10621 gccactatgc acacgttgac tgcccgggac acgccgatta tgtgaaaaac atgattactg
    10681 gtgctgctca aatggacggc ggtatcttgg ttgtttcttc tgctgacggt ccaatgccac
    10741 aaactcgtga acatatcctt ttggctcgcc aagtaggtgt tccagctctt gttgttttca
    10801 tgaacaaagt tgacatggtt gatgataaag aacttcttga gctagttgag cttgaggttc
    10861 gtgagcttct ttctaaatac gaattccctg gcgatgatat cccagtagtt aaaggctcag
    10921 ctttaaaagc tttggaaggc gatacttctg aaatcggacg cccttcaatc cttaggttga
    10981 tggaagcttg tgataactac attccaactc cagcccgtgc ggttgataag actttcttga
    11041 tgccaatcga ggacgtattc tctatctctg gtcgaggcac tgttgtgact ggtcgtgttg
    11101 agcgcggtat cgttaaagtt ggtgaagagg tggagatcgt tggtattcgt cctactcaaa
    11161 aaacaactgt aactggtatc gaaatgttcc gtaagcttct tgatgaaggt caagcgggcg
    11221 ataactgcgg tgttctactt cgtggtacta aaaaagaaga cgttgagcgt ggtcaagttc
    11281 ttgctaaacc aggaacaatt aagccgcaca aaaaattcaa aggtgaagct tatatcctca
    11341 ctaaagaaga aggtggacgt catactccat tcttcaacgg ctaccgtccg caattctatt
    11401 tcagaactac tgacgttact ggcgtttgta ccttaaaagc tggaactgaa atggttatgc
    11461 ctggtgatcg cgttgaaatt tctgtagagt taatcgctcc tatcgcaatg gaaaaagaac
    11521 tccgttttgc gatccgtgaa ggtggacgta cagttggtgc aggcgttgtt accgaaattc
    11581 ttgctgacta attgagtctt catttaactt gaagattgac agccagggga cgtctcaacg
    11641 agattgtccc cttcggcttt tgaagggttt aaaatttttt tatgcagggg tgtagctcca
    11701 gcggtagagc gaacgactcc aaatcgttag gtcgtgggtt cgaatccctc cgcccctgcc
    11761 agttctaaaa gaacttaact cttaaaatgg aaatagagag cgaaaatgga aaagacaaac
    11821 tctaaaatta tcaccctaag ctttgcgatt ggtgcttttt taactggtct tgttgttagt
    11881 ttgctaatca aagcctttgc tggtgcattt gcgattgtag ctcgagctgc agattcggat
    11941 atgttccgtc atggtgttcc tttgatgatt ggttttgcac tttttgctta tcttcagttt
    12001 aattctaaaa ttgtaacttg ggcagacgaa gtggcagttg aagtgcgaaa agtagtttgg
    12061 ccttctcgta aggacacagt tgcaatgaca atagttgtga ttgttatggt gctgatttcc
    12121 agcttgatta ttagtacctt tgatttagtt tccgctaata tcatcaacac tctcatgaga
    12181 taggaactgg ccatggataa aaaatggtac atcgtaaata ctcagactgg ttgtgaatcg
    12241 acagctaaaa cggctatcga ggagagagtt cattctcaaa aaatggaaga gttttttggg
    12301 gagattttaa ttccttctga gaacgttgtt gagctcgtaa agggtcaaaa acaaacaaaa
    12361 tcccgtaagt ttttccctgg ttacatcttt gttcagatgt ttttgaatga cgaaacgtgg
    12421 catcttgtaa aacattcatc taaggtgaca ggctttgtcg gaggtgctaa aacacgccct
    12481 ccagaagttc cagaggccga agttttgcgc gtgactcagc agatggctgg ggttgcggaa
    12541 aaaccgaagc aaaaggtgaa gttctctgtg ggagaaaatg tgaccgttgt tgacggtcca
    12601 ttctctaact ttaatggaac tgttgaagat attaatgagg acaaagcaaa agtgaaagtt
    12661 ctcgtgagta ttttcggtag accgacacct gttgaattag atttcgttca ggtagaaaaa
    12721 aattaatttt taactctcgg aagagagatt tattaacaaa ccctattgca ggagtgggtc
    12781 atggcaaaaa aagttacagg aatgatcaag ctgcagatac cggcagggaa agctaatcca
    12841 gctcctcccg ttggaccggc gctcggacag catggggtaa acatcatgga attttgtaag
    12901 cagttcaatg ctcgtacgca agcgttaggt gatagtatta ttcctatcat cattactgtt
    12961 tatcaagaca gatcgtttac tttcatcacc aaaacgcctc cagtgtcgtc tctgttgaaa
    13021 aaggcagtca aattagagtc tggctctaaa atgcctcaaa aggacaaagt tggaaaagta
    13081 aaattagagc aagtaaaaca aattgcgaca actaagttgc ctgatttgaa ttgcttaaaa
    13141 gttgagtcag caatgtctca agtcattggc actgcgaaaa gcatgggcct agagcttgcg
    13201 gactaggggt ggtgaagtat ggcaaataaa agatttaaag cagcttctaa aaaagttgat
    13261 tctacaaaaa agtacacagt tgatgaagcg ttcaaaatgg ttgtggagag tgccacagct
    13321 aaattcgacg aaacaattga tgtagctctt cgtttgggta ttgaccctaa gcaatcggac
    13381 caacaagtgc gtggcgcaat cgctcttcca catggtcttg gcaagaacgt taaagttgtg
    13441 gtttttgcaa aaggtccgaa agagactgag gctaaaaacg caggggctga tttcgttggc
    13501 gctgatgatc ttgttgctaa aatccaaggt ggttggttag atttcgacaa atgtattgca
    13561 actccagata tgatggctac tgtttcaaaa gttgctaaaa tcctggggcc ccgtggtttg
    13621 atgccaaatc caaaagttgg cactgttacc atgaacgtag gtgatgctgt cactgctgag
    13681 aaaaagggta aattagattt ccgtgttgat aaagccggta tcgtacatgc tggcgttggc
    13741 aagaaatcca tgggcgatac taaacttcgc gagaacttct tggctttgtt aggcgctatt
    13801 gtgaaagcta agcctgctgc ttcaaaaggt atctatcttc gtacaatttc tgtggcctct
    13861 actatgggtc cagggttgaa aatagatcca aatcaagcat ctggcgttgt aatctaataa
    13921 tttaaataaa ctattgcgat cagagacagt agg
//
LOCUS       DATBTE010000255         2887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2425794, whole genome shotgun sequence.
ACCESSION   DATBTE010000255 DATBTE010000000
VERSION     DATBTE010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2425794"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VIG33_09395"
     CDS             complement(<1..637)
                     /locus_tag="VIG33_09395"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY8271087.1"
                     /translation="MSLDQKVGQLFLIGFPQQSVDPQLENFIKKYKISSFILFKRNIS
                     SLSQVAKMNAQLQKISVAASVISPIIAVDQEGGQVARIPTRPPIPNAMSIGQTEDLGL
                     ARSLGQETAKIISTLGFNMNLAPVLDISNPDSQSFIGQRSFGSNADKVSSIGVALSRG
                     LLDEAVIPTAKHFPGVGSICEDPHKKVVEYNASAEAFSERDLKPFEEFSKLG"
     gene            782..1561
                     /locus_tag="VIG33_09400"
     CDS             782..1561
                     /locus_tag="VIG33_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271088.1"
                     /translation="MKKMNLRLKAYDLLTATLVCLINTLIVSHALAAEVEPIVADYRS
                     ANDLVELHYPVETNLSYKERHETAGFMFSVGYENVILDRYVSIVDFTTFYQDMFGEAE
                     FPVYGLNLSYKYNFSLGALTADFGLGYGSISDDGSGTVRSLELKKYSISGSYIMDMLF
                     SEPYFAPYVTAGIMRLGLEEKAGEEKVTGNIDMLYFYQAGFLFQLNWLDPTVSRKNII
                     DYGLENTYLDIFVSKYEPSLDVNDPDTSTDYSIGAGIRMEF"
     gene            complement(1558..>2887)
                     /locus_tag="VIG33_09405"
     CDS             complement(1558..>2887)
                     /locus_tag="VIG33_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEY8271089.1"
                     /translation="KAAAEGKVDILIGTHRLLSKDIVFKDLGLLIIDEEQKFGVTHKE
                     KIKKVRTSVDTLTLSATPIPRTLNMSLVGTRDLSLINTAPVDRLPTRTFICKFDKDTV
                     RKAISAEISRGGQVYFIHNRVQSIYGIADEVRAIVPEARIRVAHGQMNEHELEKTMLE
                     FFHHEIDVLVCTSIVESGMDIPRANTMFIDSAHMFGLSQLYQLRGRVGRSKARAYCYL
                     LMPKDKKLEKDAQERLKIIQENSSLGSGIKIAQYDLELRGSGNILGDDQSGHVNAVGY
                     ELYMDLLQEALAEAKGEETEDIELDPEINLRIQAMIPDKYISDIRIRLSYYKALADIT
                     SIDDIDRIEGELKDQFGDIPEATLNLMGVMLIRKLCKDLGIRDVSTGVKSISLIFTER
                     TLLKPETAIQLAMRENKKYSITPDNRLNIRMNNITWSAVYEELEYLLKQI"
BASE COUNT          819 a          569 c          616 g          883 t
ORIGIN      
        1 aaccaagctt tgagaattcc tcaaaaggtt taagatctct ctccgaaaaa gcctcagcgg
       61 aggcattata ttcaaccact tttttatgtg gatcttcgca gattgatcct acccctggga
      121 aatgctttgc tgtaggtata accgcttcat caagaagacc tcgagaaaga gctacgccga
      181 ttgaactcac tttgtctgcg tttgagccga acgaacgctg gccaataaaa ctttgactat
      241 ctggattgct gatatcaagc acaggggcaa ggttcatatt aaagcccaac gtactgataa
      301 ttttagcagt ctcttgacct agtgagcggg caagtcctaa gtcttcagtt tgtccgatcg
      361 acatggcatt gggaatgggc ggacgagtag gaattcgcgc cacctgcccc ccttcctggt
      421 cgaccgcaat aatgggtgag ataacagagg cagcgacgga aattttttgt agttgagcat
      481 tcattttcgc aacttgggat aatgaagaaa tatttctttt aaataaaata aaagaagaaa
      541 tcttgtattt ttttataaag ttttcaagtt gaggatctac actttgttgt gggaatccaa
      601 ttaaaaatag ctggcctact ttctggtcaa ggctcatttc ctctacggaa ggtgatgtct
      661 gagctaacac aaagttggga aataggccaa tagcaaaaaa accaataaca ccgcggaaac
      721 aaaatttcat agaataagaa taacagcttc gtccactttc gacagacgag aatatgtgct
      781 tatgaagaaa atgaacttac gtcttaaggc ttatgatctg ttaacggcga ccttggtctg
      841 tctgattaat actttaatcg taagccacgc ccttgctgcg gaagtagagc cgatagtagc
      901 cgattatcgt tcggcgaacg acttagttga acttcattat ccggttgaaa ctaacctttc
      961 atataaagag cgtcatgaga cagcagggtt tatgtttagc gtaggttatg aaaatgttat
     1021 attagatcgt tatgtttcga ttgtcgattt cactacattt tatcaagata tgtttggaga
     1081 agctgagttc cctgtatatg gacttaatct aagctataag tacaatttct ctttaggagc
     1141 gttgactgcg gattttggcc tcggttacgg ctcaatttct gatgatggtt caggaactgt
     1201 aagatctctg gaattaaaga agtatagtat atcggggagc tatattatgg atatgctctt
     1261 ttctgaacct tattttgctc cctatgtgac agctggaata atgagattgg gtttagaaga
     1321 gaaggcgggt gaggagaaag taacgggtaa tatagatatg ctttattttt atcaagcggg
     1381 ttttttattt caacttaact ggcttgaccc cacagtctct aggaaaaata ttatagatta
     1441 cggcttagag aacacctatt tagatatttt tgtttctaaa tatgagccta gtttggatgt
     1501 taatgatcca gataccagca cagactattc gattggcgcc gggattcgta tggaatttta
     1561 gatttgcttt aagagatatt ccagttcttc ataaaccgct gaccaagtga tgttattcat
     1621 tctaatattc aggcggttat cgggagtgat ggaatacttt ttattctcac gcattgccaa
     1681 ttgaatggcg gtctctggtt ttaaaagagt tctctccgta aagattaaag aaatggattt
     1741 aacgcccgta ctgacatctc gaataccgag atctttgcag agcttacgga tcaacataac
     1801 acccattaaa ttgagagtgg cttctggaat atcgccgaac tgatctttaa gctcaccttc
     1861 gatacgatcg atatcgtcaa tagaagtaat gtccgctaat gccttgtagt agcttaagcg
     1921 tatgcgaata tcggaaatat acttatctgg aatcatagct tggattctta aattaatttc
     1981 gggatcgagc tcgatatctt cagtctcttc gcctttcgcc tcagctagag cttcttgcag
     2041 caggtccatg tagagttcat aaccaacagc gttgacgtga ccggattgat catctcctaa
     2101 aatatttccc gacccacgga gctcgagatc atattgtgcg atttttattc cgctgcctaa
     2161 agatgagttt tcttgaataa tcttcaatct ttcttgcgca tctttttcaa gttttttatc
     2221 ctttggcatg agaagataac aatatgcgcg cgctttgctg cgccccacgc ggccacgcag
     2281 ctgataaagt tgactcaatc caaacatatg agctgagtct ataaacatgg tgttagctcg
     2341 cggaatatcc attcctgatt ccacaatgga agtacagacc agaacgtcaa tttcatgatg
     2401 gaaaaactca agcatggttt tttcaagttc atgctcattc atttgtccat gcgctacgcg
     2461 gatacgggcc tcgggtacga tggctcgcac ctcgtcggcg atgccataaa tactctgtac
     2521 gcgattgtgt ataaaataaa cttgtcctcc gcgagaaatt tcggcgctga tggctttacg
     2581 aacagtgtcc ttatcaaatt tacagataaa ggttctagtt ggaagacgat ccacaggagc
     2641 tgtgttaata agacttaaat ctcgagtgcc gaccaggctc atattaagtg ttcgcggaat
     2701 aggagtcgct gaaagcgtga gcgtatcgac gctggttcgc actttcttaa ttttctcttt
     2761 atgagtaacc ccaaattttt gttcttcatc aataataaga aggcctaaat ctttaaaaac
     2821 aatatcttta ctaagaaggc gatgagtgcc gattagaata tcaactttgc cttccgctgc
     2881 ggcttta
//
LOCUS       DATBTE010000256        23907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1469084, whole genome shotgun sequence.
ACCESSION   DATBTE010000256 DATBTE010000000
VERSION     DATBTE010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 23907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23907
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469084"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VIG33_09410"
     CDS             <1..407
                     /locus_tag="VIG33_09410"
                     /inference="COORDINATES: protein motif:HMM:NF015446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HEY8271090.1"
                     /translation="KNIGFEFIETVNRKLSPGNMKHHYMPPVPLILCLNIKKTEAELK
                     EIIQQRLAELPEVIEGIKLAKPPGKVHKLAHMQLSEDPVLAARSFYAQLRELAEAKPD
                     CIVLYKDPAHASERWESLYDRINKATSLIIQD"
     gene            469..1701
                     /locus_tag="VIG33_09415"
     CDS             469..1701
                     /locus_tag="VIG33_09415"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8271091.1"
                     /translation="MKSLFQDFRKLMSARFLFTLAVQMQAVILGWRMYDLMKDPLYLG
                     LIGLTEAAPAIGLALYAGYLTDRSRPLRIYKGVLTISFFSALVVLVSQILESHLSTPL
                     HSLLLYSASFLTGAARAFAQPAIFSFVPRIVARSELPRATAKMSTVMQVARIAGPAMG
                     GLLYGYIGMTNTSWLVALLLLMGSVVLFRIKANPEPLPQSGRHTSRKQELLSGVRFVF
                     SHPILLPAMSLDMISVLFGGVTALLPIFAKEILMIGPQGLGVLRAAPAVGASMMSFAL
                     TKIDLSRKAGPWLFTAVAGFGGCILIFGLSRNVYLSFLALALSGAFDSISMVIRSAAV
                     QMASPDDMRGKISAVNSIFIGSSNEIGEFESGMVARLMGTVPSVIFGGTMCLLTVAVT
                     AWWSPTLRKMDLNLIKTL"
     gene            1745..2896
                     /locus_tag="VIG33_09420"
     CDS             1745..2896
                     /locus_tag="VIG33_09420"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8271092.1"
                     /translation="MYKRSIDLYKVKSSFFLWGPRQAGKSSLLKMELPEAIYIDLLLS
                     DNFIKYNQAPFRLREELLLCKDQSSWVIIDEIQKIPALLDEVHWLLENRNFKFALCGS
                     SARKLKRGHANLLGGRALRFELFGLTAFELKKDFDLETALNRGYLANHFLHLDYKDRL
                     RSYCNDYLKEEIFAEGLVRNLPAFSSFLHTAAFSDSEQVEYATIASDVGVSAPTIKSY
                     FEILEDTLIGQFIHIYRKRPKRRLQMAPKFYFFDVGVVNSLAKRGYVEKKSELFGKAF
                     ENWVFHELRCFKSYCSPDLDIYFWKLSSGVEVDFILNDLECAIEVKATEKITSKHLQG
                     LRELKIEHPRVKRRIVVCTEKTSRITEDGIEILSYEEFIDQLWLRKIIS"
     gene            complement(2916..3908)
                     /locus_tag="VIG33_09425"
     CDS             complement(2916..3908)
                     /locus_tag="VIG33_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271093.1"
                     /translation="MDDHTYTSISREAEYFFFAENEDGYIPAEHEVIAMHLMSSMYFM
                     RNNQWEKARVEAKQASYFLETLFKSEQKHFDDPGLRIWLASIWTALGEWNEAQVDLRR
                     AYELSKNKDLLPLIDMKSPPLELTLVFEGSGPRVNWKFDSPLPTFTQKDEKPNFKITY
                     SSLPWFQRHEERNTLIRDQISKSHYMSQYYGIQLSKGGEKTMGFVSANTVRATGVLLG
                     TTIAAGGLYLVAAVLSGSGTSGGGELLTFPFIAAWYVGKSIWDQGDKIERDFNASSQK
                     IEDAGKEDLKTFRFVRFLPSWVSLTDSVVTSGTGKEITFKFQKSATAVHFLQKY"
     gene            4311..4700
                     /locus_tag="VIG33_09430"
     CDS             4311..4700
                     /locus_tag="VIG33_09430"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY8271094.1"
                     /translation="MANKLLDVGPLELEVLGILNSSGEQSVTDLQTSLKRSGHDLAYT
                     TVMTVLVRLHKKGLVTRKKQGRLFLYSAARKKDSSAHSIFEKVKNSLFRAERLTPILS
                     LLDSEEDLTREELETLKKAVEQRLKRS"
     gene            4703..5812
                     /locus_tag="VIG33_09435"
     CDS             4703..5812
                     /locus_tag="VIG33_09435"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEY8271095.1"
                     /translation="MAVIFFNLFINATFSLMISLALVLGCNRLLRANCGPWKLFLLSL
                     PFVKVIYDFLKGVPRSSILVTNIDPYRLPPGVQNFSVGIGLNNYIPQIKALFTIHDFD
                     GNKYGASVGDYVVYWLAHKLSPEFPLYVVYFISLVAVVLLARRGLSGIFFEIQRRKDR
                     LLSVSFQQRKLFLRTVDIYVSRAFSGTPFTGGVLKPYICIPNDAFENLSSQELEAVIA
                     HELGHVQQFDLLVTMIIGALGDIFWFVPGYRWLCQKLERMRELIADQRAVCGGTNPAV
                     LASALIKLKEFASGPSSMIFCSTFLYEKSLLKTRIEHLLGLLDEKPPRWAWKNIWSRS
                     LVTFWLIGAVLSSNLGGNHSTHNFTKRSVEKSAKG"
     gene            5865..6278
                     /locus_tag="VIG33_09440"
     CDS             5865..6278
                     /locus_tag="VIG33_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271096.1"
                     /translation="MNKFMQLTAIAALILVATLSEAQTLRATDIDSATWMKIFSGQLR
                     DLIVEFRQGDEIPTTLTAEGDFFETLRTQPTPLSIKKNFWMKVEQNNLLFSLDGTDFK
                     PLPQVASGTLSIGTGAGDAGGRANQLNINLKAYRK"
     gene            complement(6316..6813)
                     /locus_tag="VIG33_09445"
     CDS             complement(6316..6813)
                     /locus_tag="VIG33_09445"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY8271097.1"
                     /translation="MVASAINFILFSSAFSPNGEIPSKYTCEGQNISPPLSWQGAPAN
                     TKSFALIMDDPDAPDPQHPKTTWVHWVLFNIPANVTSLKEHIESLPEGTGEGFNDWHK
                     TGYGGPCPPIGRHRYFHKVYALDTRLTNLTHPNKAALEKAMQGHILAHAELVGTYMKG
                     GSSTK"
     gene            6974..7849
                     /locus_tag="VIG33_09450"
     CDS             6974..7849
                     /locus_tag="VIG33_09450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEY8271098.1"
                     /translation="MLNTLVLGSLDRVSTTLHSMYQMRLNRINKFKPKIEVYSEVGPF
                     VVKTVSNVQELKEALRLRYQVFHREMIGKSKGVGVDVDEFDFLCDHLAIVEKKTNRMV
                     GTYRLNCSEFSQDFYSGREFNLDRILAQPGIKLELGRACIHKDFRRGVVISLLWRGIA
                     EYMSLSQAQLLFGCASIKTENPRQAALLYKYFVSKGRIIPEYLAIPTKIYTMPKLDLW
                     IQFFQNPLTEAEIKEVEELIPPLCRAYIKIGAFIGGEPAWDAEFKCIDFLTILHREDL
                     NRTLWKRYKLDSEES"
     gene            7981..8616
                     /locus_tag="VIG33_09455"
     CDS             7981..8616
                     /locus_tag="VIG33_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8271099.1"
                     /translation="MLKVLNIEVVVKGTPVRETNFLYVGNHMGFVDIFVLASIMPALF
                     ITSQEMRETPVLGNICEMAGCIFVERRSRTQIMDELGSLKEALEQGFNVILYPEATST
                     NGEAVLPFKKTLMMAGPHAARPIQPGCINFIEINGQDFNLKSRDNVCWYGEMSFVTAL
                     WNSFTSSSMKAELHFLDPILEPPTADRTEVAKKAHALVSTHFRTPRTGEAT"
     gene            complement(8621..10456)
                     /locus_tag="VIG33_09460"
     CDS             complement(8621..10456)
                     /locus_tag="VIG33_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8271100.1"
                     /translation="MAITLIQLQNGAKGFGSRVLFDSASFAINEGEHVGVIGPNGAGK
                     TTLFKILAQQETLDEGQVTRSQQLRLGYLEQEAEWDLDEIVEDYLSKNCLKPIWDLKQ
                     LGLKLGLTEAHFSSPLTELSGGYRMRVKLLFLIGQEPNLLLLDEPTNFLDLETLLVFE
                     NFLQDFAGAFLMISHDREFLLRTTDHIVEVEAGDITKFPGNLDDYFEQKALLRDILQK
                     QMLNQESKKKSVMEFVTRFGAKATKAKQAQSKLRNLEKMEKIEIKDLPLRASIQIPEP
                     VKTGKEILSITRGECGYPGKSILKNVELRLERGKHLGIVGLNGAGKSTLLKALGGVSP
                     LLAGELKYGLNVSLAFFSQHSADQLDPDWTVLEALNHGAHSSVLQQEVLNIAGSLLFA
                     GEAVKKKVKVLSGGEKSRVALGQILLKKAPLLLLDEPTNHLDFDTVEALTEALARYEG
                     SIVTVSHDRAFIGRVANQILEVRNGGLLLYPGSYDEYVWSLQKGYLSDLNNSVNSAAN
                     IRRESSDSNNKTNNKFNYKEERKRLDASIKKMQKEITDLEKKLVEYAKKQIEMTEKLI
                     TMTGPQAQTLSKELHNLSSEIIGVEEKVLSLMEELDQAQKAVENL"
     gene            complement(10456..10812)
                     /gene="panD"
                     /locus_tag="VIG33_09465"
     CDS             complement(10456..10812)
                     /gene="panD"
                     /locus_tag="VIG33_09465"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEY8271101.1"
                     /translation="MMKCKIHRATVTDADLSYEGSISIDPRLIKATGLFINERVDIYN
                     CNSGARLSTYVIEGKKGEICLNGAAARHAQKGDLVIIVAYCDMPAEEAPKHKPTVVFV
                     DNKNRAKSIRAERKHK"
     gene            complement(10845..11615)
                     /locus_tag="VIG33_09470"
     CDS             complement(10845..11615)
                     /locus_tag="VIG33_09470"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY8271102.1"
                     /translation="MILALDVGNTQIYGGVFEKDKMLLSFRRNSKQGSSSDEVGIFLR
                     TVLRENNIDPTKVKQIAFCSVVPDVIYSLRGACKKYFNIHPFILQVGVKTGLKIKYRN
                     PVEVGADRIANSIAATHLYPNENLIIVDLGTATTFCAISKSKDYLGGSIVAGLRLSME
                     SLEAKTSKLPSVEIISTGEALGRSTIESLQSGLYYGHIGTIKEISERISKEAFDGDKP
                     FIIGTGGFANLFEKEKIFDAIIPDLVLKGLLFSLKMNA"
     gene            complement(11612..12889)
                     /gene="coaBC"
                     /locus_tag="VIG33_09475"
     CDS             complement(11612..12889)
                     /gene="coaBC"
                     /locus_tag="VIG33_09475"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY8271103.1"
                     /translation="MSKSKILFIMTGSIACYKACQVISRLVQTGAEVQVVASPSALQF
                     VGNASLEGLTGKPAVSDLYATGHLMDHIHLMRWADLIIVAPATANYINKTAQGVGDDL
                     TTTLFLAHDFKKPFLLAPAMNTSMYSHPVTQSSIKRLQEIGIEILETASGVLACGETG
                     FGKLLEPDLILKEILQRLPMTPPPQLPETIPPGDVTKTKTHPLRILVTAGGTVEPIDD
                     VRAITNLSSGLTGAKLAELMTDFGCDVHLLHAENAKAADSRVEQSTFSTFSSLQKNLK
                     FLLINREFDAVIHTAAVSDFSVANLEINGQIQIPGDFPKIHSTDTLTIHFKNNPKIVD
                     EIKSYSRNKNLKLIAFKLTSKASQEERNQAVAKLQAHSQADLVVQNDMYEIDKVKQRH
                     PFILYDRQNQVPLENIQTLATELLQNLMKEKVL"
     gene            complement(12876..13634)
                     /gene="panC"
                     /locus_tag="VIG33_09480"
     CDS             complement(12876..13634)
                     /gene="panC"
                     /locus_tag="VIG33_09480"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY8271104.1"
                     /translation="MTRVIRSPQEFKQWRRDQAGMTVGFVPTMGALHAGHESLLQRCR
                     EENEISVLSIFVNPTQFNDPKDFEKYPITWDQDLALAEKNGVDVIFYPRPEEMYPDGY
                     HYKVIENDFSNVLCGASRPGHFDGVLSVVMKLFNVVSPSKAYFGEKDFQQLTLIQGMV
                     RAFFLDLVIVPVPTMREADGLALSSRNTRLKPEERLKAPAIYKTLKAAKSPSEAAAIL
                     AEQGLKVEYVTDLKGRRFAAAFLGDVRLIDNVQI"
     gene            complement(13631..14419)
                     /gene="panB"
                     /locus_tag="VIG33_09485"
     CDS             complement(13631..14419)
                     /gene="panB"
                     /locus_tag="VIG33_09485"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY8271105.1"
                     /translation="MNILDFQEKKHKGQKISMITCYDYTFAKILNESDIDCILVGDSL
                     AMVMHGYQTTLNASVEMMALHTAAVSKGAPKKLIIGDLPFMSYRKSLSENMNGIEQIM
                     KAGAQAVKLEGAAGNLEVIRHCVESGVPVMGHLGLTPQSVNQLGGFKVQGRDEKAQAL
                     IKQYAHQLQDAGCFGVVLECVPSHLAQEISQELEIPTIGIGAGLACDGQVLVLQDMLG
                     MNEGFSPKFLRKYFNGFEEIKKAFNKFHSDVIHKEFPNEKESYS"
     gene            complement(14416..15129)
                     /locus_tag="VIG33_09490"
     CDS             complement(14416..15129)
                     /locus_tag="VIG33_09490"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEY8271106.1"
                     /translation="MTKMLIIGAGKLAQHLNHYLHILELSPLSWDRTQDPLLLKTKIS
                     QASHILLAISDSAIENFYRTQLAGFDKTVVHFSGALHIPDLIAAHPLMSFGPQLYDRD
                     TYFRIHFTLTGADSLLDALPGLPNSFAVLVPEKKALYHSLCVMGGNFPVILWQKMLGE
                     FESLGISKEAARVYLETILDNTLRNPQTALTGPLARKDKQTVMKNLEALKGDEFQKIY
                     LAFIDTVTPGLLEKKKRDL"
     gene            15275..15994
                     /locus_tag="VIG33_09495"
     CDS             15275..15994
                     /locus_tag="VIG33_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271107.1"
                     /translation="MKYLYWAVAAVVIMVGVGLSVYWGQETVSLPKIVFNYYRNPGQF
                     AETIHVQMKPELKSSPLLMLGVMPGRKIDLEIWKSFLDQTSSELKYQVVIVDPDLPFI
                     KELFPNAIKMDLKKDIARFIAGAKNAQAQGLRMAVLVPSIYASQLLQSNPAENIKKNS
                     DLQPISFSIIGFPRSPEQEANMEIPCAMGQNDRDGEGALGCAVQKKARLLYRKKSKPG
                     FYEGVLDQVGDKDYLVLFNAP"
     gene            complement(15991..17367)
                     /locus_tag="VIG33_09500"
     CDS             complement(15991..17367)
                     /locus_tag="VIG33_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IG family 5'-nucleotidase"
                     /protein_id="HEY8271108.1"
                     /translation="MTGKVYVNRTLNLKRIRYIGFDMDHTLVRYNSENFERLSHTTMI
                     DKLIKGKGYPEALRKLLFDYKFAVRGLVIDRQKGNLLKLNRYTAIRASYHGMKPLDFK
                     IHQKLYKSTYIDLSNPNYMAVDTSFSISLAVLFGQIVDLKDSDKNGTYPEYAQIADDV
                     LDALDEAHRDGSLKDEVKKNLVHYIIKDESVVRGLEKFVKHGKKLFVLTNSDFHYSKL
                     LLDYAVNPFLKDGKTWHDLFEVVVTLASKPKFFYEDQKFLVVNPQDGTMTNLNNKFGA
                     GIFQGGNAKKFTVDLGLNGDDILYIGDHIYGDILRLKKDCNWRTAMVIEELTEEIANN
                     KEAEPTNQEIETLMRKKEPLEDDLTDLMTRKIEKQADLNEATIEQLQKAIAEIDTQIS
                     QLIKKQQALYNERWGQLMRAGNEESYFAYQLDRYACVYMTKISDLLELSPRTYFRAPR
                     RPLSHEMY"
     gene            17488..18729
                     /locus_tag="VIG33_09505"
     CDS             17488..18729
                     /locus_tag="VIG33_09505"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIF1 family ATP-dependent DNA helicase"
                     /protein_id="HEY8271109.1"
                     /translation="MTHTLAPTLSQEQAHALELLRSGENVFLTGGAGSGKSYLIRHFM
                     NELDPKQTPILASTGAAAVLLGGRTFHSFFGLGIMEGGAEATLERCSKDHRLLNRLKK
                     VEGVIIDEISMIPGAALMIAEMLSQKARGSKLPWGGMRVICVGDFAQLPPVTRAGPRD
                     WAFLNEVWSRSGFQVCQLSHNQRVQDNSFLDVLADVRYGKVSPRVFDFLSEKVLPHNE
                     EDSGTRLFGRRDVSEKFNQKKLAEINETEVTIDSIYLGSEKHIEALMKMSPVPQKLTL
                     KVGCRVIFLQNDPQKRWINGTRGTVTKIEIDKITVKKDHGREVSVDKTTFSLQDAEGN
                     VTATVIQFPLTLAYATTIHKSQGATLDEVWCDLRSLWEPGQAYVALSRLRTAKGLKLV
                     GWNPRSIIVDPKVLGFYNGLE"
     gene            18780..20051
                     /locus_tag="VIG33_09510"
     CDS             18780..20051
                     /locus_tag="VIG33_09510"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8271110.1"
                     /translation="MNIKKNLWKIILLFLIGLGLGWGGQCLFESRKTEQTITRLEQPP
                     ATLLPSLEELYPYLIPPNSTLNSVLRELDVSSQTIHQIVEAAKPVSDLGRLKPGVRFQ
                     LTYVLPADPSSEVVGIRFLFSPAEMLEVKKTQDKWFAQKITEVVEKKVMTFSGVVTSS
                     LWSSAAEAKMDPNLISDLAEVFAWQLDFSREVQVKDHWRLSVEQKLVKGQPIGWGSIL
                     AAEYESETQKYAGVLYRIDENQSGYFTPEGVSLRRMFLKSPIRYGRISSRFTTKRFHP
                     ILQFNRPHLGVDYAAPIGTPVRAVGDGVAIAVGTHGGAGKMIRIRHNSEYATAYKHLS
                     GFAKGVRTGARILQGQIIGYVGNSGLSTGPHLHFEFFQGGRYVDPLGRKFPQAEPIPV
                     HLLTKFKSEVIGLMASLPEWNSLSQGSQSRF"
     gene            complement(20048..20491)
                     /locus_tag="VIG33_09515"
     CDS             complement(20048..20491)
                     /locus_tag="VIG33_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271111.1"
                     /translation="MKKIISTLLVSTILSSAPVMATETETPLPQNLGDMGITAQDLNT
                     QEGIADPSKDTEMDATQWGYRRRCGWGSYLQRYVIRIGRFSYIRYRCVRYGGRYPGYP
                     RPGYPRYPRYPRYPGYYGTIENQSTPQDQSNVQEPVEVQEESVAH"
     gene            20680..21813
                     /gene="ribD"
                     /locus_tag="VIG33_09520"
     CDS             20680..21813
                     /gene="ribD"
                     /locus_tag="VIG33_09520"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY8271112.1"
                     /translation="MKMTSEQAMRMAMLEASKGATFVSPNPMVGAVVLDSKENFLASG
                     YHEFYGGPHAEVNALKHLSPEKLRGATVFVTLEPCAHQGKTPSCAQMMAKLPLKKVIY
                     GLVDPNPLVAGKGAQILRGAGIATEIFSDTHPEFKPDLEETCEAFLWNFRQNKIFVSL
                     KIAQSLDGQMGLKSGESKWITNEKAREHVHYLRACHDATLVGRGTVKIDNPVLNIRHP
                     RIEKTNKVVIIDPSGSLLNEASTLQVTHHHNSQNIFWCVNDTKFKVSTVSDATLSRAT
                     ILKIKANSEGSLDLPDLLQNLWNQGLRSVMIEGGAMTASKFLSAGLVNRLYLFQAPIV
                     IGAEGGKSWTESLNILEMKQRQILKNPKTMAFDGDLLVTGRLS"
     gene            22064..22420
                     /locus_tag="VIG33_09525"
     CDS             22064..22420
                     /locus_tag="VIG33_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271113.1"
                     /translation="MKNEWALFWESIAGEGQTGDAFETGKLEVLNREQLNDIIKALSQ
                     DRKRLNQKLESLHKEIELNSAKLESIRLVGGRDEEILKRINELSDIGQVLSEQLNKLD
                     EKIKWTRNRQENFLTE"
     gene            22635..>23907
                     /locus_tag="VIG33_09530"
     CDS             22635..>23907
                     /locus_tag="VIG33_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microtubule-binding protein"
                     /protein_id="HEY8271114.1"
                     /translation="MSFNYHKNKEGHSQDSFWTSYSDLFLGLSTIFLLLYVISSLRTS
                     TDTIRYQVESQKLSMEVNELKGQLKMYENVKNEYMNNAPKDEVQEYQELMDKLTLLQE
                     DAKSEKDKLIEQALENDKKGKALNKYQQMVRNVLNANKMAKSKILHRDDLIKDQDETI
                     EDQDNQIGDLQEDIQQKKELIAEGERKIDEANNSLKTKTNELKQALRTNKITQKKYDE
                     RIAQARAENDKRVNQLALANEKYSEQLEATTGQLNQVSAQLHQTNTQLAKTQGVLAQK
                     ENETRGLYGQLKKQGDDAQAKIAGLQAGFAAEKARERELFDAELNKNKAMGAAERARR
                     EGEFKAAMAAKERDLGGKIAALGGQLKNTEGQLAKAKAEIEARKSVADEVKKGFAKAG
                     IKADIDMETGDVVLDFGQAYFDNDSDHLKSEM"
BASE COUNT         6555 a         5079 c         5490 g         6783 t
ORIGIN      
        1 caaaaaacat tggatttgaa tttatagaga cagtaaatag aaaactgtct ccgggaaata
       61 tgaagcatca ctacatgcca ccggtgcctt tgatattatg cttaaacata aaaaagaccg
      121 aagcagaact gaaagaaata atccagcaaa gacttgcaga gcttcctgaa gttattgaag
      181 gaattaaact cgccaaacct cctggaaaag ttcataaact ggcacatatg caattgtcag
      241 aagatccagt tcttgccgca cgaagctttt atgctcaact gagggaactg gcggaagcaa
      301 agcctgattg catcgttctc tacaaagatc cagcccatgc cagtgaacgt tgggaaagtc
      361 tttatgaccg tattaacaaa gcaactagct taattatcca agactaaggt ctaagcaatt
      421 cactaatatg gaaatcttta aggcaaagtt gataatccag ggttttaaat gaagagtttg
      481 tttcaagatt ttagaaagct gatgtccgca cgatttctat ttacgttagc agtgcaaatg
      541 caagcggtga ttcttgggtg gcgtatgtac gatctcatga aagaccctct gtacttggga
      601 ttgattggct tgaccgaagc cgctccggcc atcggtctgg ctctgtatgc gggctacctg
      661 acagatcgca gtcggcccct tcgaatttat aaaggcgttt taaccatcag ctttttttca
      721 gcactcgtgg tcctggtctc acaaattctt gaaagccatt taagcacacc cttacattct
      781 ctgctccttt acagtgcttc cttcttgacc ggggccgcca gggcctttgc tcaacccgca
      841 attttttctt ttgttccgag aattgttgcg cgcagtgaac ttccgagggc cacagcaaag
      901 atgagcactg ttatgcaagt ggcgcgcatc gcgggtccgg caatgggcgg tctcctctat
      961 ggctatattg gtatgaccaa cacctcatgg cttgtggccc ttttgttgct gatgggaagt
     1021 gtcgttcttt ttagaattaa agcaaatccc gagccgctcc cacaaagtgg gcgccatacc
     1081 tctcgcaagc aagaactttt atcaggagtg cgttttgttt tcagtcatcc gatcttgtta
     1141 ccagcaatgt ctttagacat gatttctgtt ttatttggtg gtgtaaccgc gctattaccg
     1201 attttcgcaa aagagatttt gatgattggt ccgcaggggc taggtgtttt aagggcggcc
     1261 cctgccgtgg gcgcctctat gatgagcttt gctcttacta agatagatct cagccgaaaa
     1321 gcgggccctt ggttatttac agcggtggct ggttttgggg ggtgtatttt aatcttcggt
     1381 ttgagtcgaa atgtttattt atctttcctt gccctagctc tgagtggagc tttcgacagt
     1441 atcagcatgg tgattcgttc tgcagctgtg caaatggcat ccccggatga tatgcgcgga
     1501 aaaatatcgg cggtgaattc catttttatt gggtcgtcca atgaaatcgg ggagttcgaa
     1561 tcgggaatgg tcgcccgcct tatgggaaca gtcccctccg tgatctttgg tgggacaatg
     1621 tgtctgctca ctgtggcagt caccgcctgg tggtcgccga ctttacgtaa aatggactta
     1681 aatcttataa aaacactatg aaaatcattt acttagcata aataaagtgt tactttcgaa
     1741 ttacatgtat aaaagaagta ttgatttata caaagtaaaa agctcttttt tcttgtgggg
     1801 tcccagacaa gctggaaaga gttcactcct taagatggag ctaccagagg ctatctatat
     1861 tgatcttttg ctttctgata atttcataaa atacaaccaa gcgccgttcc gattacggga
     1921 agagttactt ctttgtaagg atcaatcatc ttgggtaatt attgatgaga ttcaaaagat
     1981 cccagcctta cttgatgagg ttcactggct ccttgaaaat agaaacttta aatttgcact
     2041 ttgtggttcg agtgcaagaa aattaaaacg tgggcacgcc aatctgttag gaggccgggc
     2101 cttgcgattt gaacttttcg gtctcactgc atttgagtta aaaaaagatt ttgatcttga
     2161 aaccgctttg aacagaggtt acttggcgaa ccactttttg catctggatt ataaagatcg
     2221 attgcgttcg tactgtaatg attatcttaa agaggaaatt tttgctgagg ggttggtccg
     2281 taatttgcca gccttcagct cttttttgca tactgcggct ttttctgatt cggagcaggt
     2341 cgaatatgcc acgattgcaa gtgacgttgg agtttcagca ccgacaatta aatcttactt
     2401 cgaaattctt gaagatactc tgattggtca gtttatacat atctatcgta aacgaccgaa
     2461 aagaagactt caaatggcgc caaaatttta tttttttgac gttggagttg taaattcact
     2521 ggcgaagcgc ggctatgtgg aaaaaaaatc tgagctattt ggcaaagctt ttgagaattg
     2581 ggtgtttcac gagctgcgtt gttttaaatc atactgttcc ccagacttgg atatctattt
     2641 ctggaaatta tctagcggtg tggaggttga tttcattcta aacgatcttg agtgtgcgat
     2701 tgaagtgaaa gcgacggaaa aaattacaag caagcatttg cagggattga gagaactcaa
     2761 aatagagcat cctagggtca aacgtcgaat cgtggtctgc accgaaaaga cttctcgaat
     2821 tactgaggat ggaattgaaa ttctctctta tgaagagttc atagatcagc tatggcttag
     2881 aaaaataatt tcttaataaa tttccaatag cgaccttaat atttctgtaa gaaatgaaca
     2941 gcggtcgctg acttctgaaa tttgaaagtg atttcctttc cagttcctga ggtcacaaca
     3001 gaatcagtta aggaaaccca actcggtaaa aatcgcacaa agcggaaggt ttttaaatct
     3061 tctttaccag catcttcaat tttctgagaa gacgcattga aatccctttc aattttgtct
     3121 ccctggtccc atatggattt accgacgtac cacgcggcaa taaagggaaa ggttaataat
     3181 tctccaccac cgcttgtgcc gctaccgctc aatacagccg caacaagata aaggcctcct
     3241 gcagcgatcg tggttccgag gaggacccca gtggctcgga ctgtattggc agagacaaag
     3301 cccatggttt tttctccgcc tttgctgagc tggatcccat agtactggct catataatgg
     3361 gattttgaga tttgatcgcg gattaaggta ttgcgttctt catgacgttg aaaccaaggc
     3421 aacgatgaat aggtgatttt aaaattgggc ttctcatcct tttgtgtgaa agttggcaac
     3481 gggctatcga atttccaatt tacacgggga ccactgcctt caaaaacaag ggtgagttca
     3541 agcgggggag acttcatatc aataagcgga agcaggtctt tgttttttga aagttcatag
     3601 gcccggcgca aatcgacctg agcttcattc cattctccca acgctgtcca gatactcgcc
     3661 aaccaaatgc gcaaccctgg atcatcgaaa tgtttttgtt cggatttaaa aagcgtctcc
     3721 aaaaaatagg aagcttgttt agcttctacg cgggcttttt cccactgatt attgcgcata
     3781 aaatacatcg aactcatcag gtgcatggca atcacttcat gttcggcggg aatataccca
     3841 tcttcattct ctgcaaagaa gaaatattcg gcctcgcggc tgatactggt ataggtgtgg
     3901 tcatccaatg tgcggctttg tttgaggaga tctttttgat ttgtcttatc tgcccaaaga
     3961 cctaaccaag atttttcaat ggaagtgaca aagccgccag cttctttttt tggaaattcc
     4021 gccagagcct tttcggggtc tgaaaactta aaggcggcct ctgaatattt gtattcagaa
     4081 tcgcgaatga tctgtgaggt tgtgcaagcg cagagtcaga ggggcagtaa aaacagggtg
     4141 aaattcttca taatgagagg ataatagaga gggtttttta gaacaagcaa agcctttatc
     4201 actccgcaat agcttttgta agcacttgaa atgaatgtat tttccagata tataggctta
     4261 tctactacaa tcgtagttga caaatactac ggttgtagta gtttttactc atggcaaata
     4321 aacttttaga tgtgggtccc ttagagcttg aagtccttgg aattctgaat tcctctggag
     4381 aacagtcggt gaccgatctg caaaccagtc ttaagagatc aggtcatgac ttggcttaca
     4441 cgacggtgat gaccgtttta gttcgtcttc ataagaaggg cttagttact agaaaaaaac
     4501 aggggcgttt atttttatac tcggctgcca ggaaaaaaga ttcttcagcc cattctattt
     4561 ttgagaaggt aaaaaactct ctattcagag ctgaacgtct gactcccatt ttgagcttgc
     4621 tggatagtga ggaagacctt acccgtgaag aactcgagac cctcaaaaaa gccgttgaac
     4681 aaaggctcaa gaggtcttag gcatggcagt tatctttttc aaccttttta ttaacgccac
     4741 tttttcactg atgataagtc ttgctttggt gttgggttgc aatcggttac tgcgggccaa
     4801 ctgtggtcct tggaagttat tcctattgtc tttgcccttt gttaaagtta tttacgactt
     4861 cctaaaggga gttccccggt cctccattct ggtgacaaac attgatccct atcgacttcc
     4921 tccgggagtg cagaattttt cagtaggcat cggtctcaat aattatattc cacaaattaa
     4981 agctctgttt accattcatg attttgatgg gaacaagtat ggagccagtg tgggtgatta
     5041 cgttgtctat tggttggctc ataagctgag tcccgaattc cctctctatg tggtctattt
     5101 tatctccttg gttgcagtgg ttttgttagc acgccgtggc ctctcaggga tattttttga
     5161 aattcaacgc cgcaaagatc gtctgctgtc ggtgagtttt cagcagagaa agctttttct
     5221 gcgcacagtg gatatttatg tttcgcgcgc tttctcgggc acacctttta cgggcggagt
     5281 ccttaaacca tacatttgca ttccgaacga tgcttttgaa aacctatctt ctcaggagtt
     5341 ggaggcggtg attgcgcacg agttaggcca tgtccagcaa ttcgatcttt tagtgaccat
     5401 gattattgga gctttaggag atattttttg gtttgtgccg ggttatcgtt ggctctgcca
     5461 gaagctagag cgcatgcgtg aactcattgc ggatcagaga gccgtatgtg ggggaacaaa
     5521 tcctgcagtc ttggcttccg ctttgattaa gctgaaggaa tttgcaagcg gtccctcttc
     5581 catgattttt tgctccacat ttttatatga aaaatctctt ttgaaaaccc gcattgagca
     5641 tcttctgggt ctcctggacg aaaagcctcc gcgctgggcc tggaaaaata tctggagtcg
     5701 gtcgctagtg accttttggc tgattggcgc ggtgttaagt tcgaatctag gcggcaatca
     5761 ttcgactcat aatttcacaa agcgctcagt ggaaaagtct gcaaaaggct aagtattttt
     5821 ttgaatttaa ctactacata tgtagtaata acaaggagaa gaccatgaac aaatttatgc
     5881 aactaaccgc cattgcagct ttgatcttag tggccacact atctgaagca caaactttgc
     5941 gggccaccga tatagatagt gcgacgtgga tgaagatatt ttcaggacaa ctgcgggacc
     6001 ttatagtgga gttccgccaa ggggatgaga ttcccaccac acttaccgcc gagggcgatt
     6061 tctttgagac tttgcgaacc cagcccaccc ctttaagtat taaaaagaat ttttggatga
     6121 aggtagagca aaataatttg ctctttagtc tcgacggcac tgactttaag cccctccccc
     6181 aagtggccag cggaacattg agcatcggga ctggagcagg tgatgctggt ggtcgcgcga
     6241 accagctcaa cattaacctc aaggcttacc ggaaatagaa aggagccaaa ggaatgtaga
     6301 acgaaaaggc gggtcttatt tcgtagaaga tccccctttc atataagtcc ccaccagctc
     6361 cgcatgagcc agaatatgac cctgcatcgc tttttctaac gccgctttat ttggatgcgt
     6421 aagattcgtg agtcgggtgt cgagggcata gactttatga aagtaacgat gacgaccgat
     6481 cggagggcag ggcccgccgt aaccggtttt atgccagtca ttgaagcctt cacctgttcc
     6541 ctcaggcaag ctttcgatgt gctccttgag cgaggtcaca ttcgctggaa tattaaagag
     6601 cacccaatgc acccaagtcg tttttggatg ctggggatcc ggagcatcgg gatcatccat
     6661 gatcaaagca aaacttttgg tattagcggg agccccttgc caagacaaag gtggagaaat
     6721 gttttgtccc tcacaggtgt atttcgaagg aatttctccg ttaggagaaa aggctgatga
     6781 aaacagaata aaatttattg ccgatgcgac cataagtcct cctctctcaa tcataaatcg
     6841 cttagcttgc gacgtatagt ttctttacct agattcccca gactcgattg tgctgaataa
     6901 aatactaacg gactcctgac ccaaccttaa catgaagttc gctaccatta gaattaacca
     6961 aaaggaggat ttgatgttga acactttggt tcttggaagt ttagatcgtg tttccacaac
     7021 cttacactct atgtatcaaa tgcgcttgaa tcgcattaat aaattcaaac cgaaaattga
     7081 agtttactct gaggtcggtc cgttcgtggt caagactgtg agtaatgtgc aagagctcaa
     7141 agaagctctt cgacttcgct atcaagtttt tcatcgcgag atgatcggta aaagcaaagg
     7201 agtgggtgtc gatgtcgatg agttcgactt cctttgtgat cacttggcga ttgtcgaaaa
     7261 gaaaacaaat agaatggtag gaacttatag attgaattgc tccgagtttt cgcaagactt
     7321 ttactctgga cgtgaattca acctggatcg catccttgcc cagccgggga ttaagctgga
     7381 gcttggacgc gcgtgtattc ataaagactt ccgcaggggc gtggtgattt cgctgctgtg
     7441 gagaggaatc gctgagtata tgtctttaag ccaggctcaa ctgctcttcg gctgtgcgag
     7501 tatcaaaact gaaaatccgc gacaagcggc tttgctttat aaatattttg tcagtaaggg
     7561 tcggattatt cctgagtacc ttgcgattcc gactaagatt tacacgatgc cgaagttgga
     7621 tttatggatt caatttttcc aaaatccttt gacggaggcg gaaataaaag aggtggaaga
     7681 gttgattccg cccctctgtc gagcttatat taagatcggt gcctttattg gcggggaacc
     7741 cgcttgggac gccgaattta agtgcattga tttcttgacg attcttcacc gtgaagattt
     7801 aaataggacc ctgtggaaac gatacaagct ggattccgag gaatcataag aattttttta
     7861 ttattggttg gaatagttac cttttttacg agaggcttat acatcaaatg gcgtcatcgt
     7921 catgatacgg tcgccagccg taagggtttc agtgaaaatg cgcagtctca ctgcgcctat
     7981 atgctcaaag ttttaaatat cgaagtggtc gttaaaggca cccccgtgag ggaaactaat
     8041 ttcttgtatg tgggaaacca catgggattt gtcgacattt tcgttttggc ctccatcatg
     8101 ccagctcttt ttatcacctc ccaagaaatg cgtgagaccc ccgtgctggg gaatatctgc
     8161 gaaatggctg gctgtatttt tgtcgagcgt cgcagtcgca ctcaaatcat ggatgagctt
     8221 ggctctttaa aagaagccct cgagcagggc tttaatgtga ttctttatcc cgaagcaact
     8281 tctactaatg gtgaggcggt tttacctttt aagaaaactt tgatgatggc gggaccgcat
     8341 gccgctcgcc cgattcagcc aggatgtatc aatttcattg aaatcaacgg ccaagatttc
     8401 aacttaaaat cccgcgataa tgtttgttgg tatggggaga tgagttttgt caccgcactg
     8461 tggaactcat tcaccagttc ttcaatgaaa gcggaattgc actttcttga tcctattctc
     8521 gagcctccga cggcagatcg gacggaagtg gctaaaaaag ctcacgctct ggtgagcacc
     8581 cactttagaa ctcctcgaac tggggaggcg acttaggact tcaaagattt tccacagctt
     8641 tttgggcttg atctaattct tccataagac tcaacacttt ttcctcaacc ccaatgatct
     8701 cactggaaag attatgcagt tccttggaaa gcgtctgcgc ctgggggccg gtcatggtaa
     8761 tcagcttttc cgtcatctcg atttgctttt tcgcgtactc cacgagcttt ttctctaggt
     8821 ctgttatctc tttctgcatt ttcttaatgg aagcgtctaa gcgctttcgt tcttctttgt
     8881 aattgaattt gttattcgtt ttattattgg aatcagaaga ttcccttcga atgttggctg
     8941 cggaattcac agagttattg agatcagata aataaccttt ttgcagactc cagacatatt
     9001 catcataaga gccggggtag agaagcaatc ccccatttcg aacttcaaga atttgattcg
     9061 ccactcgccc gataaaggcg cgatcatggc tcacggtcac aatactgcct tcgtatcttg
     9121 ccagtgcctc agtcaaggct tcgacggtgt cgaagtccaa gtgattcgtg ggctcatcca
     9181 gcaacaagag cggagccttc tttaaaagaa tttgacctag ggccacacgg gatttctctc
     9241 ctccggaaag aacttttact ttctttttca cagcttcgcc ggcaaaaagc aaagaacccg
     9301 cgatattcaa aacttcctgt tgcagaaccg aagagtgtgc gccgtggttc agggcttcaa
     9361 gcacggtcca atcaggatcc aattgatccg cactgtgctg agaaaaaaac gccaggctta
     9421 cattcaaacc gtatttgagc tctccggcca gcaggggact cactcccccc agtgctttga
     9481 gcaaagtgga ttttccagcg ccattaagac cgacaatccc taaatgcttc ccacgctcta
     9541 ggcgtagctc cacattttta agaatgctct tgcctggata cccgcactct cctcgagtaa
     9601 tgctgaggat ttctttgccc gttttcacag gctccgggat ttggatcgaa gctctgagcg
     9661 gcaaatcttt aatttcgatc ttttccattt tttcgagatt gcgcagttta ctctgagctt
     9721 gtttagcttt tgtggccttc gcgccaaagc gggtgacgaa ttccatcacg gattttttct
     9781 tggactcttg gttgagcatt tgcttttgta aaatatcacg gagcaaagct ttctgctcaa
     9841 agtaatcatc tagattcccc gggaacttag tgatgtctcc ggcttcaact tcaacgatat
     9901 gatccgtggt gcgaagcaag aattcccggt catgggaaat catcaagaaa gctccggcga
     9961 aatcctgcag aaaattttca aaaaccagca aagtctccaa atccaagaaa ttcgtgggct
    10021 catccagcag caaaagattt ggctcttgcc caatcaggaa aagcagcttc acacgcatgc
    10081 gatagccacc actgagttcg gtcaacggag aactaaagtg agcttcagtt agaccaagct
    10141 taagccctag ttgcttcaga tcccagatgg gcttaagaca gttttttgag agatagtcct
    10201 ccacgatttc gtcgagatcc cactcagctt cttgctccaa ataccccagg cgcaattgtt
    10261 gtgagcgggt gacctggcct tcatcgaggg tttcttgctg ggccaggatc ttaaacaaag
    10321 tggttttgcc agccccgttg ggaccaataa cccctacgtg ctcaccttca ttgatggcaa
    10381 agcttgcgga gtcaaaaaga acgcgggagc caaaaccttt ggcgccgttt tgaagttgga
    10441 tcagtgtaat agccattatt tatgttttcg ctcggcgcgg atggattttg cgcggttctt
    10501 gttatccaca aagacaacag tgggtttgtg tttcggagct tcctcagcag gcatatcaca
    10561 ataggcgacg atgatgacca gatccccttt ttgagcatgg cgagccgccg ctccattcag
    10621 acagatttct ccctttttgc cttcgatcac ataagtggaa aggcgggcac cgctattgca
    10681 attataaata tccactcgtt cattaataaa taaacccgtt gctttaataa gccgagggtc
    10741 aatggaaata gagccttcat agctgagatc tgcatcagtg acagtggctc tatggatttt
    10801 gcatttcatc atggttacat tcatagtctt ttccgttccg ccagttatgc attcatcttt
    10861 aaggaaaaca ataaaccttt taatactaaa tccgggataa tcgcatcaaa tattttttct
    10921 ttttcaaaga ggtttgcaaa acctcctgta ccaataataa agggcttgtc accgtcaaag
    10981 gcttccttcg aaatgcgctc gctgatttcc ttgatagtac cgatatggcc gtaataaagg
    11041 cccgattgaa ggctttcaat ggtggatcgg ccgagggctt cccctgtgga aataatctca
    11101 acagacggca gcttagaggt tttagcctcc agggactcca tcgagagccg caaacccgcg
    11161 acgatggagc ctcctaaata atctttgctt ttggaaatag cgcaaaaagt ggtggcagtc
    11221 cctagatcca cgataattaa attttcattt ggataaagat gagtggcggc aatggaattg
    11281 gcaattcgat ccgccccgac ttcgacagga ttacggtact tgattttaag ccccgtttta
    11341 actcccactt gaagaataaa gggatggata ttgaaatatt ttttacaagc tccgcgcaaa
    11401 gaataaataa catccggcac aactgagcaa aaagctattt gcttcacttt tgtggggtca
    11461 atattatttt cccgaagcac cgttcttaaa aaaatcccga cctcgtccga ggaagagcct
    11521 tgtttagagt ttctgcggaa agagagcagc attttgtctt tttcaaaaac gccgccatag
    11581 atctgcgtat taccgacgtc aagggcgagt atcataggac tttttccttc ataagatttt
    11641 ggagaagttc agttgcaagt gtttggatgt tttcgagagg aacttgattt tgacggtcat
    11701 ataagatgaa aggatgtcgc tgtttaacct tatcaatctc atacatatcg ttttgtacaa
    11761 ctaagtcagc ctgggagtga gcttgcaatt tagcgacagc ctggttgcgc tcttcctggg
    11821 aagccttact cgtcagttta aaggcgatca gtttaagatt tttattgcga ctgtaagatt
    11881 tgatttcatc tacaatcttg ggattatttt tgaaatgaat cgtcaacgtg tcagtagagt
    11941 gaattttcgg aaaatcaccg ggaatctgta tctggccatt gatttcgaga ttggcgacag
    12001 agaagtcact gaccgccgca gtatggatga cggcgtcgaa ttcacgattg attagtagaa
    12061 atttcagatt tttttgcaga gaagaaaaag tgctaaatgt actttgctcc acccggctat
    12121 cggccgcttt tgcgttttcc gcatgcagta agtggacatc gcagccgaag tccgtcatga
    12181 gttccgcaag tttcgccccg gtcaagccag agctaagatt ggtgatggcg cggacatcat
    12241 caattggctc caccgttccc ccagcggtga ccaaaattcg caaaggatgg gtctttgttt
    12301 tagtgacgtc accgggtgga atcgtctctg gaagctgcgg tggtggtgtc attggaagtc
    12361 tttgtagaat ctcttttaaa atcagatctg gctctaaaag tttgccaaat ccggtctcac
    12421 cacaggctaa aaccccagaa gctgtttcta atatttcgat cccaatctct tgcaggcgct
    12481 ttatcgaaga ctgagtcacc ggatgagaat acatgctggt attcattgca ggggcgagca
    12541 agaaaggctt tttaaagtcg tgggctaaaa atagggtagt ggttaaatca tcacccacac
    12601 cttgagcggt tttattgata taattcgctg ttgcaggtgc cacaataatc agatccgccc
    12661 aacgcatcag atggatatga tccatgagat ggccagtggc atataaatca ctgacagcag
    12721 gctttccggt gagtccctca aggcttgcat tgccgacaaa ttgcaacgca ctcggcgagg
    12781 ccaccacttg aacttcggca cctgtctgaa ctaaacgcga gatcacctga caggctttgt
    12841 agcaggcaat ggatccggtc atgataaata gaattttaga tttggacatt atcaatcaac
    12901 ctcacgtctc ctaagaaggc cgcggcaaag cgacggcctt ttaaatcggt tacgtattct
    12961 acttttaatc cttgctctgc caaaattgca gcggcttcgc tagggctttt tgcggctttt
    13021 aaagttttgt aaatagctgg agccttcagc cgttcctccg gcttcagacg cgtgttgcgc
    13081 gaacttaaag caaggccatc ggcttcgcgc attgtcggca ctggcacaat aaccaaatcc
    13141 agaaagaagg cgcggaccat tccctgaatc aaagtcagtt gttgaaaatc tttttctccg
    13201 aaataagcct tagacggcga aaccacatta aagagtttca tcaccacgga gagaacaccg
    13261 tcaaaatgac cgggacggga ggccccgcaa agaacattgc taaaatcatt ttcaatgact
    13321 ttataatgat aaccatcggg atacatctct tcgggtcgag gatagaaaat tacatccacg
    13381 ccgttttttt cagccaaggc caaatcttga tcccacgtaa tgggatactt ctcaaaatcc
    13441 ttcggatcat tgaactgagt cggattcacg aagatgctga gcacggagat ttcattctct
    13501 tcgcggcaac gctgcaacag actttcatgg cctgcatgca gagcgcccat tgtgggaaca
    13561 aaacccacgg tcatgccggc ttgatcgcgg cgccattgtt tgaattcttg gggactgcga
    13621 atgactcgag tcatgagtaa ctctcttttt cattggggaa ttctttgtgg ataacgtcgg
    13681 agtgaaactt gttaaaagct ttttttattt cttcaaaacc attaaaatat ttgcgtaaga
    13741 atttcggaga gaaaccttca ttcatcccaa gcatatcttg cagcactaaa acctgcccgt
    13801 cgcaggcgag gccagcgccg atgccaatgg tggggatctc cagttcttgg ctgatttcct
    13861 gagccaagtg tgaaggcaca cattccagga ccacaccaaa gcatcccgca tcttgcaact
    13921 gatgggcgta ttgtttaatc aaagcttggg ctttttcatc acgaccttga actttgaatc
    13981 cgcccagttg attcactgat tgcggagtca aacctaaatg tcccatcact ggaacaccag
    14041 actccacgca gtgacgaatc acctcgaggt ttcccgccgc gccttcgagt tttacggcct
    14101 gagctccggc tttcatgatt tgttcaatgc cattcatgtt ttcggaaagt gatttccgat
    14161 aggacataaa aggaagatcc ccgatgatca attttttagg agcccctttg gaaaccgcgg
    14221 cggtatgcag tgccatcatt tccacagagg cattgagagt ggtctgatag ccatgcatca
    14281 ccatcgctaa gctgtcacct acgagaatac aatcaatatc actttcgttt aaaatcttgg
    14341 cgaaggtgta atcgtagcag gtgatcattg agatcttttg tcctttgtgc tttttttctt
    14401 gaaaatcaag gatgttcata aatctctctt tttcttttcc agaagtcctg gagttacagt
    14461 gtctataaaa gctaaataaa ttttttgaaa ctcatcgccc ttcagagcct cgaggttttt
    14521 cataacggtc tgtttatctt tacgcgcgag ggggcccgtc agtgcggtct gcggattcct
    14581 caaagtattg tcgaggatgg tttcgagata aactcgggcc gcttctttag aaattcctaa
    14641 agattcaaac tctcccaaca ttttctgcca aaggatcacg gggaaattgc ctcccattac
    14701 gcacagggaa tgatagaggg ccttcttctc gggaactaag actgcaaagc tattgggaag
    14761 gcccggcaaa gcgtctagaa ggctgtcagc gcctgtgagt gtaaaatgga tgcggaagta
    14821 agtgtcgcga tcataaagct gtggtccgaa actcattaaa gggtgagccg cgatcaggtc
    14881 gggtatatga agtgctcccg aaaaatggac cacggtttta tcaaaacccg caagttgagt
    14941 gcgatagaag ttttcaatag cggaatccga aatggccaag aggatatggg aggcctgaga
    15001 aattttagtt tttaaaagaa gaggatcttg tgtgcgatcc cagctgagcg gcgaaagctc
    15061 aagaatatga agataatggt ttaagtgctg agcaagtttg cctgcaccaa taattaacat
    15121 ctttgtcata acgtctggta cctgtctggt tcacaacctt ttgatcaagt cctatgtttt
    15181 tagagtggca caatttagac gtgttttcca ctaaaaacaa gcctttcttc agctgaagct
    15241 tgctaagccg caaacaggat gttacccccc tgttatgaaa tatttatact gggctgtagc
    15301 cgctgttgtg atcatggttg gtgtgggttt atcggtttac tgggggcaag aaacggtcag
    15361 tcttccaaag attgttttta attactatag aaatcctggc cagtttgccg aaaccattca
    15421 tgtgcaaatg aagcctgaat taaaaagttc ccccctcctt atgctgggcg tgatgcccgg
    15481 acgtaaaata gatctcgaaa tatggaaatc atttttagat caaacctcct ccgagcttaa
    15541 ataccaagtg gtgattgtag atcccgacct tccttttatt aaggaactct ttccaaatgc
    15601 cattaaaatg gacctaaaga aagatattgc gcggtttatt gcaggagcca agaatgccca
    15661 ggctcaaggt ctccgcatgg cagtcctcgt acccagtatt tatgcttcac agcttttaca
    15721 aagtaatccc gcagagaaca ttaaaaagaa ctcggatctt cagccaataa gtttttcaat
    15781 cataggattc ccacgttcgc ccgagcaaga ggccaacatg gaaatcccct gtgccatggg
    15841 acaaaatgat cgcgatggag agggagccct gggttgcgcg gtccagaaaa aagcccgtct
    15901 tctttatcgc aaaaaatcga aacctggttt ctatgaaggt gtcctagacc aggtcggcga
    15961 taaagattat ctcgtgcttt tcaatgctcc ctagtacatt tcatgggaca gcggacggcg
    16021 aggcgcccga aaataagttc gcggtgacaa ctccaataaa tccgaaattt tagtcatgta
    16081 gacgcaggca tagcgatcga gttgataagc aaaataactc tcttcattgc ctgcccgcat
    16141 gagttgaccc cagcgttcat tgtaaagcgc ctgctgtttt ttaatgagct ggctaatttg
    16201 cgtgtcaatt tcagcaatcg ctttttgcaa ctgttcaatg gtcgcttcat ttaaatcagc
    16261 ctgcttttca attttccgag tcatgagatc cgtcagatca tcctcgagcg gttctttttt
    16321 tcgcatcaga gtttcgatct cttgattggt gggctcggcc tctttgttat tcgcaatttc
    16381 ctcggtcagt tcttcgatga ccatggcggt tcgccaattg cagtcttttt tcaaacgaag
    16441 aatatcgcca tagatatgat ctccgatata aaggatgtcg tccccattta agcccaagtc
    16501 cacggtaaac ttcttcgcgt ttccaccttg gaagatgcca gcgccaaatt tattatttaa
    16561 attggtcatg gttccatcct gtggatttac cacgaggaat ttttggtctt catagaagaa
    16621 tttaggtttt gaggccaagg taacaacaac ttcaaaaaga tcatgccaag tttttccgtc
    16681 ctttagaaaa ggattaaccg cataatccaa caaaagcttt gagtaatgga aatcagagtt
    16741 tgttaagacg aaaagttttt taccatgttt aacaaatttc tccaggccac ggaccacgct
    16801 ctcgtcttta ataatataat gaacgagatt ttttttaact tcatctttca agctaccatc
    16861 gcgatgagcc tcgtctaatg cgtctaaaac gtcatcggca atttgagcat attcaggata
    16921 agtgccattt ttatcgctat ccttgaggtc gacgatttga ccaaagagaa ccgccaagga
    16981 tatagaaaaa gaggtatcca ccgccatata attaggattg ctcaaatcga tgtaagtgct
    17041 cttgtagagc ttctgatgaa ttttgaaatc caaaggcttc atcccatgat agctggctcg
    17101 gattgcagta tagcgattga gcttgagcaa gttccctttc tgtcgatcta tcaccaggcc
    17161 acgaaccgcg aatttgtagt cgaaaagaag tttccgaaga gcctcaggat agccctttcc
    17221 cttaatcaat ttgtcgatca tagtggtgtg agaaagtctc tcgaagtttt cactgttata
    17281 gcgaaccaaa gtgtggtcca tgtcgaatcc aatgtagcga attcttttga gattgagggt
    17341 tctatttacg taaacttttc cagtcataga aaaaaaatcc tttttttaaa gggaacgaag
    17401 agggatagat ttatattgcc gctagcccct gcgttatagt caaaagtatt atattatcgt
    17461 agtatacttg cgacagcaag gaaactgatg actcataccc tagcccctac actttcacag
    17521 gaacaggcac atgctttaga acttcttcgt tcaggcgaaa acgttttttt aacgggcgga
    17581 gcgggaagcg ggaaaagcta tttgattcgt cattttatga atgaattaga tcccaaacaa
    17641 actcctattc tggcctccac tggagcagcg gccgtccttt taggagggag aacttttcac
    17701 agtttttttg gtctaggcat tatggagggg ggcgctgagg ccactttaga gcgctgttcg
    17761 aaagatcatc gacttctcaa tcgtctgaaa aaagtcgagg gcgttatcat cgatgaaatt
    17821 tccatgatcc ctggagcggc cctgatgatc gcggaaatgc tttcgcaaaa agctcgcggc
    17881 tcaaaacttc cctggggcgg tatgagggta atttgtgtag gcgattttgc tcagttaccg
    17941 ccggtgactc gagcaggtcc tcgagattgg gcttttttaa atgaagtttg gtcgcggagt
    18001 ggttttcaag tttgccagct ctcgcataat cagcgggttc aggacaatag tttcctcgat
    18061 gttcttgcgg atgttcgcta tgggaaagtg agccctcgag tttttgattt tctcagtgaa
    18121 aaagttttgc ctcataacga agaagattcg ggcactcgac tttttgggcg ccgagatgta
    18181 tctgaaaaat tcaatcaaaa aaaattggcg gaaattaatg aaacggaggt caccattgat
    18241 tccatttatt tgggttctga gaaacatatt gaagctttga tgaagatgtc tcctgtgcct
    18301 cagaagctca ccttaaaagt tggttgcaga gttatttttc tgcaaaacga tccacaaaaa
    18361 cgttggatca atggcactcg tggtaccgtt accaaaatcg aaatcgacaa gatcactgtt
    18421 aaaaaagatc acggacgtga agtcagcgtg gataagacaa cttttagttt gcaagacgcc
    18481 gaaggcaatg tgacggcaac agtcatccaa tttccgttga ctctcgctta tgcaaccaca
    18541 attcataaaa gtcagggagc aaccctggat gaggtttggt gtgatcttcg ctccctctgg
    18601 gaaccgggac aagcttatgt ggcactcagt cgcttgagaa ccgccaaggg cttaaaatta
    18661 gtggggtgga atccacgttc aatcatcgtg gatccaaaag ttttgggatt ttataacggg
    18721 ttagaataaa attggaagaa agcttcacct tcctgtgatt ttttgcctat aataagtcag
    18781 tgaatataaa aaaaaatctt tggaaaatta ttttactttt tttgattggg ttgggattgg
    18841 gttggggagg acaatgtctt ttcgaatcac gcaaaacaga acaaacaata actcgtctag
    18901 aacagcctcc tgcaaccctt ttaccatcgt tagaagagct ttacccttac cttattcctc
    18961 cgaattcaac cttaaatagt gttttgcgag agcttgatgt cagttcccag accattcatc
    19021 aaattgttga agcggcaaaa cccgttagcg atcttggacg tttgaagccg ggggtgcgtt
    19081 ttcagctgac ctatgtactt ccagcggatc cctcttctga ggttgtaggg attcgatttc
    19141 ttttttcacc agctgagatg ctagaggtca aaaagacgca ggataaatgg ttcgcgcaaa
    19201 aaattactga agtggttgaa aagaaggtca tgactttttc tggggtggtg acaagctctt
    19261 tatggagttc ggcagccgaa gcgaaaatgg atcctaatct catctctgat cttgccgaag
    19321 tttttgcctg gcaattggat ttttcccgtg aggtccaagt caaggatcac tggcgcctct
    19381 ccgtcgaaca aaaattggtg aagggtcagc ctattggatg gggctctatt ctggcagcgg
    19441 aatatgaaag cgaaactcaa aagtacgctg gggtgcttta tcgcatagat gaaaatcagt
    19501 caggttattt tacccctgaa ggcgtcagtt tgcgtcggat gtttttaaaa agtcctattc
    19561 gctatggccg aatttcttct cgttttacca ccaagagatt tcatccgatt ttacaattca
    19621 atcgtcccca tctaggtgtg gattacgccg cccccatcgg cactccggta agagcggtgg
    19681 gcgatggtgt cgcgatagct gttggcaccc atgggggtgc tggtaaaatg attcgtattc
    19741 gccataattc tgaatacgca accgcttata aacatctcag tggttttgct aaaggagttc
    19801 ggactggggc acgtatttta caaggccaaa tcatcggcta tgtaggcaac tcaggtcttt
    19861 ccaccggacc tcatcttcat tttgaatttt tccaaggggg gcgctacgta gatcccttgg
    19921 gccgtaaatt ccctcaggca gagccgattc ctgtgcacct tctgacaaag tttaagtcag
    19981 aagtgatagg tttaatggct tcactgcccg agtggaatag cttatcccag ggttctcaat
    20041 caagattcta gtgcgcaact gactcctctt gaacttcaac aggttcctga acattcgact
    20101 gatcctgagg tgttgattga ttttcaattg tgccataata tcctggataa cgaggatatc
    20161 ttggatagcg agggtagcca gggcgaggat agccaggata acggccgcca tatctcacgc
    20221 agcgataacg tatgtaagaa aaacgtccaa tacgtataac atatcgttgt agataagatc
    20281 cccatccaca gcggcggcga tatccccatt gcgttgcatc catctctgta tctttggatg
    20341 ggtcagcaat accttcttgg gtatttaagt cttgggccgt gattcccata tcgccgagat
    20401 tctggggtaa cggagtctcg gtttcagtcg ccattactgg agctgaagat aaaattgttg
    20461 aaacgagcag cgtagaaata atttttttca tattgttcct ccttgaattg aacatgggcc
    20521 tagtatgggg acggttgatg aagtctttat gagaaactct tgaaaaaatt gattgtttat
    20581 atctgtattt taaagtcgtt gtgcccttgg acaattcaga atatgccatg tggccagttc
    20641 catcttgaat tcacttctaa ggaggggtag ttttcgctta tgaaaatgac ttcagaacaa
    20701 gcaatgagaa tggctatgct agaggcttcc aaaggagcca ccttcgtaag tcccaatcct
    20761 atggtgggcg ctgtcgtcct ggatagtaaa gaaaattttc tcgccagcgg ataccatgaa
    20821 ttttatggtg gaccgcatgc ggaagtgaat gctcttaaac atttatcccc tgaaaaactg
    20881 aggggtgcca ccgtattcgt gactttagaa ccttgtgctc accaagggaa gaccccttct
    20941 tgtgctcaga tgatggcaaa actccctttg aaaaaagtta tttatggttt ggtggatcca
    21001 aatccattag tggctggcaa aggggcgcag attttgcgag gcgccggaat cgcaaccgag
    21061 attttttccg acacccaccc tgaatttaaa cctgaccttg aagaaacgtg tgaagcattt
    21121 ttgtggaatt tccgccagaa taaaattttt gtttctttaa aaatagcgca aagtcttgac
    21181 ggtcagatgg gacttaaaag tggcgaaagc aaatggatta ccaatgagaa agcccgcgaa
    21241 catgttcatt acttgagagc ctgtcacgat gccacccttg taggccgagg caccgttaag
    21301 attgacaatc cggtcttgaa tattcgccat ccacgaatcg aaaaaacaaa taaagtcgtc
    21361 attatcgatc caagtgggag cctattaaat gaagcttcaa ccttacaggt gacccatcat
    21421 cataatagcc agaatatttt ttggtgtgtg aatgacacaa agttcaaagt gagtactgtg
    21481 tctgacgcca ctctttcaag ggcgacaatt ctaaaaataa aagcgaactc tgaaggttca
    21541 ttggatttgc cagacctctt acagaatctt tggaatcaag gtcttcgttc agtaatgatc
    21601 gaaggggggg caatgacggc ttctaagttc ctaagcgctg gtctggtgaa tcgtctttat
    21661 ctttttcaag cccccattgt tatcggtgca gaagggggta agtcgtggac ggagtccttg
    21721 aatattcttg aaatgaagca acggcaaatt ttaaagaatc caaaaactat ggcttttgat
    21781 ggagatcttc tagtcactgg gcgtttgtcc tgaaaatttt acaggacgca agtccgtacg
    21841 ctagaggtca attgtcgggc ggcagcgata tgtttatcct aataaagcgt atattcgaat
    21901 aggcgacatc aacgcaaagc atttatttct agactttctc cagcgtagca agtgaagagc
    21961 tgtctgtgct aaaatctatt ccataataat aaggttttac ggattgagga gttcttaagt
    22021 atttatgggc gaagagggta gtaatagatc aatagtcaaa aatttgaaaa atgaatgggc
    22081 tcttttctgg gagagtattg cgggagaagg ccagactggt gatgcttttg aaacgggaaa
    22141 gttagaagtt cttaatcgag aacagcttaa tgacattatc aaggctttaa gccaagaccg
    22201 taaaagatta aatcaaaagt tagagtctct gcataaagag atagaactta atagcgcaaa
    22261 gcttgaaagc atccgcttgg taggtggtcg agacgaagaa atcctcaaac ggattaatga
    22321 actaagcgac ataggccagg ttttgagcga gcaacttaat aaattggatg aaaagattaa
    22381 gtggaccaga aaccgccaag aaaactttct gactgaataa atcatagaca ttatagacta
    22441 aattagaatt tccttagcca agccatccga tagttcctag aacaaggtct aggatgactc
    22501 tctctggcac tcctaattga aaatgaattt ttgcagcgtc cccctccaga ctccagtttt
    22561 tacgggagat aaccgttaac ttggttagta gcggttattg agatcgttaa agtttgtgct
    22621 ggtggtggtg aaatatgtcg ttcaattatc acaaaaataa agagggacat tcacaagaca
    22681 gtttttggac gtcttattcg gatttatttt taggtctgag taccatcttc ttgttgctct
    22741 atgtgatttc aagcttacgc acgagcacgg atacaattcg ctatcaagtt gaatctcaaa
    22801 agctctctat ggaagtcaat gagctcaagg gacaactcaa aatgtacgaa aatgtgaaaa
    22861 acgagtacat gaataatgct ccaaaagatg aagttcaaga atatcaagag ttgatggata
    22921 aactaaccct tttacaagaa gacgcgaaaa gtgaaaaaga taagttgatc gagcaagctc
    22981 tcgagaacga caaaaaaggc aaagctctta ataaatatca acaaatggtt cggaacgtat
    23041 taaatgcaaa taaaatggcg aagtctaaaa ttcttcaccg tgatgatctt attaaagatc
    23101 aagacgagac tatcgaagat caggataacc agatcggcga tttacaagag gacattcaac
    23161 agaaaaaaga gttgattgct gaaggtgaaa gaaaaattga cgaagccaat aactctctaa
    23221 aaacaaaaac gaatgagcta aaacaagcat tacgtacgaa taaaattacg caaaaaaagt
    23281 atgatgaacg tattgctcaa gcccgtgctg aaaacgacaa gcgggtgaat cagttggctt
    23341 tagcgaatga gaaatattcg gaacaactgg aagccactac gggccaatta aatcaagtca
    23401 gtgctcaact tcatcaaacc aatactcagt tagcgaagac tcaaggcgtg cttgctcaaa
    23461 aagagaatga gactcgtggt ctttacgggc aattaaaaaa gcaaggggat gacgctcaag
    23521 cgaaaatcgc gggtcttcaa gctggtttcg ctgctgaaaa agctcgcgaa cgcgaattat
    23581 ttgatgccga gttaaataaa aataaggcga tgggcgctgc tgaaagagcc cgccgcgagg
    23641 gtgagttcaa agcggcaatg gccgctaaag agcgggatct gggcggtaag atagcggctc
    23701 ttggtggcca attaaagaat acagaaggtc agttggcaaa agccaaggct gaaatcgagg
    23761 cccgtaaatc tgtagcggat gaagtgaaaa aaggttttgc gaaagcaggg atcaaagcgg
    23821 acattgatat ggaaacagga gacgtggtcc ttgatttcgg tcaagcttat tttgataatg
    23881 actcggatca tttaaaaagt gaaatga
//
LOCUS       DATBTE010000257        13090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2220384, whole genome shotgun sequence.
ACCESSION   DATBTE010000257 DATBTE010000000
VERSION     DATBTE010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 13090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13090
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2220384"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1768
                     /locus_tag="VIG33_09535"
     CDS             <1..1768
                     /locus_tag="VIG33_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271115.1"
                     /translation="TGIPSLQEVSAKPSTASVFYNNLEDTTQTLPKTKHLLPTTEFHY
                     ETESHRLRIPRYMAYLLGNEEFLNKFPEYRILVPEFEKVITLANAHDIEKAPKHVYTK
                     LAHDKGIDYDKLPPKEAAKAKAFRTAFNADGEMKITKLMKTQGFIKESGEINQKGKLF
                     FTLEAFVDRWDAYKFRAQEFGKEMLRPGEYIRKVLKPSSFTTDHYNIDKVAAMDDFVT
                     AEPDKVNLEKAVGRVTPQDYKRMRTRFRGSDPSFGDIQHEQATKLIAEHQEKAQVKKT
                     LKTPSPVNAVEVEKGKAPRSAISKSAASKLAAHRMVNGIMDIGTAGAVAYAIRDATDP
                     KKELGESLNSAGVSAWNIGSAIGTAGAGVVSTLGDDMADRYTDQKVYEDYLNLPLEEQ
                     EKIFRDNNTLAFRNVIFSKRPRIAEMRCSAKTSGSGSSQSSPLEFDVYSPERQTIHMK
                     IFFDNNKPMYLIGKPKSPSSSEGSEIRLGLQSQQACFSLPQYSREQRAFITQNKCTTI
                     NAKGLPDLEPEMAQYITPDYWGEMVKTHDWALKHISQLQNCCNSKECLKVMNNERYLA
                     SLDKERGVTKLESPTNSVKGKR"
     gene            1910..5206
                     /locus_tag="VIG33_09540"
     CDS             1910..5206
                     /locus_tag="VIG33_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271116.1"
                     /translation="MRDSNLFNKLLLTYLILSAFLMSCTRATKESKSNISFSIPQSVS
                     SKKVGAQAFDTLIHVSINISGSGIQTPIVFNWDSEKKGLAGTTTTAPTSFSFDIPQGP
                     DRLIQVLAVYGDSTSPNSDGGAMQFYYGDVTKTLAAAVEDAPVTVASVGQGTAIISGQ
                     IQGRYLTSAGEGPTGQVDIVYIPPGGKTPMVVERSMIMNGWFQFFGLLGAQLGYRLQD
                     GTFLWGGPVDLSEASFPPSGQVMRAALPIHLKTDNYSGTVTTHQEDPSISIYGFFGDS
                     TLVSTKYICKSVSSLTYLLKVGTATALTVTDNNSADPSDLFDTTLSSVNLRGGVALGG
                     ASPCDTQENIASNLFGSVLGFKSSMLESGRDNSAGFRLPFQLNYANNSSGSIFSSTVV
                     DADKISISGTLLPGTPSILDNFSVYKAINLGLNFHYHSSFAPCESLTTMGYTLVGDTP
                     VSGGTYTATLPLTSTEASGGAMFVLCPTKAGAPIGPGVWVNASYLRGGGGGGGGGGGV
                     AAKIAFMNLSAITVNQCGHVNLSLLTGADSPTSSANPVTVNLAKTGTGSEFYAENDYN
                     CAGNPITSVVIPANSNWLGVNFKTSATPETDITLTPTDAASALTSVAKTIRARATGSV
                     TEMRFVANSQSANVGTCFPMSVKAVDSNGNLISYNGAVSVTSSGATLYTDSSCSVTSG
                     GTLSFAAGVASFYIKGTTAGWVNLQASATIDTLNFVANYGMAISSAGQPTHLVLGTPS
                     PSYLYINQCMPITVTANDDTNAEAPMPTTQVIKFTASGGGAGNNFYTDANCTNSMVNA
                     ETGITSGQTSINIYFKPQNSGSLGLSGFIDNMNYSIGGSQNYTIGSLWMHVISGGFNG
                     SWFDNTCGSLTFELMDNSIAGSGNVIPNLSGSPVPINITTNASTVSGGLYSVAGCTDS
                     VAASKSTAIGSGASTSVIYMNSLLSSGSYVSVTPSYSGSYSPTYQNGNTIASTSFAWC
                     PPVDGGGACASSLALSGPNVAGGVGLFTPGITVGSSMDKTITITNTSSSGVSLGTMTI
                     TGGQAAEYTFKGGTYPGTGGTCTGYISPSGTCTVVVTFTPAAINGRYASLNVNYNGTS
                     LPLSLQGTGL"
     gene            5376..6647
                     /locus_tag="VIG33_09545"
     CDS             5376..6647
                     /locus_tag="VIG33_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEY8271117.1"
                     /translation="MSSAMWFALAGALLIVMAVISAALKRLPLTTAIVYLTIGILLGD
                     SLLGVISLDLLRDVKLLRLLTEIAVIISLFTAGLKLRLPLDSMEWRVAFWLATFSMLI
                     TIFLMTLFAHLVFGFSVGAAILLSAILAPTDPVLASSVQVLGPEDQDRLRFSLTAEAG
                     LNDGIAFPFVMLGLGLLGLRDLGEWGLRWFSIDLLWGVSGGILIGTLLGAGVGKVVGY
                     LRAKHEEAESFDDFLALGLIGLSYGVAVLLHAYGFLAVFAAGFAIRQRERQESRSFRP
                     MLDKSEGLLRTDTVSGHMALGVLEFNEQMERIGELIVVTALGALLSRENLRLEYFWFI
                     PVLFFIVRPISVLIGTGLNKGGHKILISWFGIRGIGSLYYLAFVIEQGLPAELEKKIS
                     AITFSMVAASILVHGLSSTPLMIRYARGNRR"
     gene            complement(6679..6816)
                     /locus_tag="VIG33_09550"
     CDS             complement(6679..6816)
                     /locus_tag="VIG33_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271118.1"
                     /translation="MKSQNVSQIEGFILILLSAFLIMSFTGIDKAHAESIQPLPEHSE
                     I"
     gene            complement(7082..8008)
                     /locus_tag="VIG33_09555"
     CDS             complement(7082..8008)
                     /locus_tag="VIG33_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271119.1"
                     /translation="MKIASLSFALFLSLALTSRCFAALAAQLFFNNEENPVMTAGEAT
                     NLKIEVFNSDSPDPVHHFHPMHEKEMHLILISEDLESFAHLHPQHLSDHIGIFGIDLN
                     QPTQDPYNLDAISAVKRPGPYFLFAESMPMGFSMTTLPLDFRASGVATQKKPLKIDPV
                     SSDGFIYKNFNDYQVKLKVNTYPHCGNFAALLEMELKHLDPLSQTYLDVTDLQPWLSS
                     FGHTIMLSEQGTIAKEKKFIHLHAVWPLVDDPETERGPYLRVASDSHAPMTEGIFKIW
                     VQFKHHDQVQTIPFVVEIKAPLTPSHANIQCL"
     gene            complement(8005..9084)
                     /locus_tag="VIG33_09560"
     CDS             complement(8005..9084)
                     /locus_tag="VIG33_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HEY8271120.1"
                     /translation="MMRFVRSVFVLFCLTIVPIHQIYALSPQIQGMNFIAQVNEHAND
                     KYSALWGYTAPDGREYAILGVRTGTAIIDITDNNNVKEVAFIKSVRSLWREMKTYSHY
                     AYVVTDNADTGIEIIDLAQLPSSATLVSVYKDLPMSHTITIDEPRKLMYLMGGSGETV
                     VILSLEDPLKPKEIARFGSSYVHDAYIKNGRAYLSEIMSQTFSIWDIADTANPKFLKR
                     VRDKDAPAVSFHNSWTTEDEHYLVTTEETQDRTVKIWDISDLNAIKVIAEWLPPNRLA
                     HNVQIKGNYAYFSSYGGGIRILDLSNPLHPNEVAFWTRSEEVEKGFVGMWACYPFFKS
                     GKVIGSDIENGLIVVDFEGAREAVR"
     gene            9352..9834
                     /locus_tag="VIG33_09565"
     CDS             9352..9834
                     /locus_tag="VIG33_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271121.1"
                     /translation="MKMKFTCMAALLGFATTSWAGLPTEAFGRYHVVSYVENGRTIPM
                     KDSGALENASAEVKNRTEVYNFSFNTSDGHTFSVDTLGIYEAAFYGVTADAIEIVKNE
                     VKTKELTTDLDSLIRFGKCERITKTHFAYDSAFSWIKITSYMKLCNGKEYERELLMNK
                     "
     gene            complement(9849..10643)
                     /locus_tag="VIG33_09570"
     CDS             complement(9849..10643)
                     /locus_tag="VIG33_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271122.1"
                     /translation="MKQLPLNWKNKFTTLREIIPDPQAALALNAQLMKNWGADESAQR
                     ITAAALHGMPELNAAILKEFNLNLAKFFFYFNRAWEGASEIGTSARLEWWKTKGPSLP
                     SHIKYYNIGGVLDQPNQTFFQKGIGYGWRLNTVDDRFLTFNHRELFNVGKTDGLPFEF
                     AGSRINDSQVDLYKTFLWPSVARELTNGHCFDSKLVGVARTHHWGLTLPYAFVNVDAQ
                     KKPIANPFPRDVLLHALVDAITHDLATTENICATPKPSSNVANTEN"
     gene            11175..11315
                     /locus_tag="VIG33_09575"
     CDS             11175..11315
                     /locus_tag="VIG33_09575"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEY8271123.1"
                     /translation="MESTSPRSFRANLKEYMEMADENPIRIQKREGNACVLMSEEYYE
                     AY"
     gene            11493..11825
                     /locus_tag="VIG33_09580"
     CDS             11493..11825
                     /locus_tag="VIG33_09580"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY8271124.1"
                     /translation="MTDANDVLKKRILSQFQVSEEDLIKLCQAMGVKSLAIFGSALQG
                     NFKPGQSDIDFLVEFELVSIDCFFDFLEGLKRLFHYTDIDLVTRGSLKNQVIREEILS
                     SKKDLYAA"
     gene            11815..12144
                     /locus_tag="VIG33_09585"
     CDS             11815..12144
                     /locus_tag="VIG33_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEY8271125.1"
                     /translation="MPRKLEAYVEEILESIAQIESFVRGIEFAEYLQDDRTKAAVERK
                     LLNIGEALNQASQVSQEIETKVTEFRKIVGFRNVLVHGYFGVDDALVWDIIATKLKRL
                     KSDVESL"
     gene            complement(12288..12752)
                     /locus_tag="VIG33_09590"
     CDS             complement(12288..12752)
                     /locus_tag="VIG33_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271126.1"
                     /translation="MDFIDGLILSHGEEEPFLSLKLNKCLKLKQGEISMKKLILATVL
                     LASAFANAEDRKVYNVITKQSDIVSVLSNLIQLEKEGVAQRSGTMYDLTALSLSGYQV
                     VKITFRDSFGDGKYCSADLTFNKSDINDNQFMSFMNMKQDANRWTIFVPYCK"
     gene            complement(12773..12931)
                     /locus_tag="VIG33_09595"
     CDS             complement(12773..12931)
                     /locus_tag="VIG33_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271127.1"
                     /translation="MDKYCFLTRYEIECLERTLEEMIQLFPLVPIAQIESDMVEIQEN
                     CPEMGNLD"
BASE COUNT         3568 a         2700 c         3122 g         3700 t
ORIGIN      
        1 tacgggaatc ccttccttac aagaagtcag tgcaaagccc tcaactgctt ctgtttttta
       61 taataatctt gaagatacaa ctcaaactct gccaaaaaca aaacaccttt tgcctaccac
      121 ggaatttcat tatgagacgg aatcgcatcg tttgcgaata cctcgttata tggcttattt
      181 gctgggcaat gaggagtttt taaataaatt tcctgaatat agaatcctcg ttccggagtt
      241 tgaaaaagtg atcactttag ccaatgcgca tgatatcgaa aaagccccta agcatgtcta
      301 tacaaagtta gctcatgaca agggaattga ttacgataag ttgcctccaa aagaagcggc
      361 aaaagccaag gcttttcgaa ctgcattcaa tgcagatgga gaaatgaaaa taacaaaatt
      421 gatgaaaact cagggcttta tcaaggaatc tggtgagatc aatcaaaagg gaaagttatt
      481 ttttacgctt gaagcttttg tggaccgttg ggatgcgtac aagttccgtg cccaagaatt
      541 tggcaaggaa atgttgcgtc ctggggagta tatccggaaa gtattaaaac catcgagttt
      601 tacgacggat cattataaca tagataaggt tgccgcgatg gatgattttg taactgcgga
      661 gcctgataaa gtaaacttgg aaaaggctgt gggaagagtt actccccagg attataaaag
      721 aatgcggact cgtttcagag gcagtgatcc ttctttcgga gatattcagc acgagcaagc
      781 gacaaaattg atcgcggaac atcaggaaaa ggctcaggta aagaaaacac ttaagactcc
      841 cagtccagtg aatgccgtgg aagtggaaaa aggaaaagcc ccacggtctg cgatttcgaa
      901 gtccgccgct tcgaaattgg cagcgcatcg aatggtaaat ggaattatgg atatagggac
      961 agccggagct gttgcctacg caatccgtga tgccacagat cctaaaaaag aattgggtga
     1021 atccctcaat agtgcaggtg tttcggcgtg gaatataggt tctgcaattg ggacagcggg
     1081 agcgggtgtg gtaagtactt tgggcgacga catggctgat cgctatacgg accaaaaagt
     1141 ttatgaggat tacctcaatt tgccgctgga agaacaagag aagattttca gggataataa
     1201 tactcttgcc tttagaaatg ttattttttc caaaaggccg aggatcgctg aaatgcggtg
     1261 ctcagcgaag acaagtgggt cagggtcctc tcaatcatct cccttggagt tcgatgtcta
     1321 cagtccggag cgccagacga ttcatatgaa aatatttttc gacaataata agcccatgta
     1381 tcttattggc aaacctaaat cgccgtcttc ctccgaaggg agtgaaattc gtctcggctt
     1441 acagtcccag caagcctgtt tctctttgcc tcaatattcc cgagagcaac gggctttcat
     1501 cacacaaaac aaatgcacca ccatcaacgc aaaaggtctt ccagaccttg agcccgagat
     1561 ggctcaatat atcactccgg attactgggg agagatggtg aagacccatg attgggccct
     1621 taaacatatt tcccagttac agaactgctg taatagcaaa gaatgtttaa aagtcatgaa
     1681 caatgaaaga taccttgctt ctttagataa ggaaagagga gtgacaaaac tggaaagccc
     1741 tacaaattcc gttaaaggga aacgctaata agtttttctt ataaaatctc aaaataagaa
     1801 ttttattttt aaatcgaata tagtggatgc ccaacaaaaa atttggactt cttatcatgt
     1861 ctttcggtta ggattccgac aagtcattgt tctaatccaa ggagaggtta tgagggattc
     1921 aaatttgttt aacaagctgt tgctgacgta tctgatcttg tccgcttttt taatgtcgtg
     1981 cacccgagcg acgaaagaat ctaaatccaa tatttctttc tctattccac aaagcgtgag
     2041 ctcaaaaaaa gtgggggcac aagcttttga tacgctgatt cacgtttcga tcaatatcag
     2101 cggcagcgga attcaaactc ctattgtatt taattgggat agcgaaaaaa aaggactagc
     2161 gggcacaacc acgacggctc cgacgtcctt ttcttttgat attccgcagg gacctgaccg
     2221 tttaattcaa gttttggctg tttatggtga ctccacttcc cccaatagtg atggaggagc
     2281 catgcagttt tattacggag acgtgacaaa aactttggcg gcggccgttg aagacgcccc
     2341 tgtcactgtc gcttccgtgg ggcagggaac agccattatc tccggtcaaa tacagggtcg
     2401 ctatctgacc agtgccggag aaggtccgac gggtcaagtg gatatcgttt atattcctcc
     2461 agggggcaaa actccgatgg tggtggagcg ctcaatgatc atgaatgggt ggtttcaatt
     2521 ttttggtctg ctgggagctc aattgggcta tcgactgcaa gacgggactt tcttatgggg
     2581 tgggccagtg gatttatcgg aggcttcgtt tccaccttct ggtcaagtta tgcgggcggc
     2641 tcttcctatt catttgaaga cggacaatta ttcagggaca gtcaccactc atcaagaaga
     2701 cccaagcatt tctatctatg gattttttgg tgattccact ttggtgagta caaaatatat
     2761 ttgtaaatcg gtctcttctt tgacttattt gttaaaggtc ggcacggcga cggcacttac
     2821 agtgactgat aacaacagtg cggatccaag cgatttattt gatacaacac tgagctccgt
     2881 gaatcttcgt gggggcgtgg cacttggagg agcctctcct tgcgatactc aagagaatat
     2941 tgcgtccaat ctatttggat cagtcctggg attcaaatcc agtatgcttg aaagtggccg
     3001 cgacaacagt gcgggattta ggctgccgtt tcagctgaat tacgcaaata atagcagtgg
     3061 cagtattttt tcttcgacgg tggtggatgc ggataaaatt tctatttcag gaactctttt
     3121 gccgggaact cctagcatct tggataattt ttccgtctac aaagcgatca acctaggttt
     3181 gaactttcac tatcattctt cttttgcacc ctgcgaatct ttaactacga tgggttatac
     3241 tctcgtaggc gacacgccag tcagtggagg gacttatacg gcaactctgc ctttgacttc
     3301 aaccgaagcc agtggaggag ccatgtttgt cctttgtccg acaaaggccg gtgcacccat
     3361 tgggcctgga gtgtgggtca atgccagtta tctgcgtggt ggcggtggcg gtggcggtgg
     3421 cggtggcgga gttgccgcca agatcgcttt tatgaatttg tctgccatca cggtcaatca
     3481 atgtggtcat gtcaatctaa gtctgttgac tggcgccgac agtcccacct cttcagctaa
     3541 tcctgtcaca gtgaatctgg caaagacggg aactggaagt gaattttatg ccgagaatga
     3601 ttacaattgt gcggggaatc ctatcacttc agttgtcatt cctgcgaact cgaattggtt
     3661 gggagttaac ttcaagacaa gcgccactcc cgaaacagac attactttaa ctccgacgga
     3721 tgcggcttca gccttgactt cagttgcaaa aaccattcga gccagagcaa caggttctgt
     3781 gacagagatg agattcgttg cgaattccca atctgcgaat gtcgggacct gttttccgat
     3841 gtccgtgaag gcggtggatt ccaatggaaa tttaatttct tacaatggcg ctgtaagtgt
     3901 gacgagttca ggtgccactc tctacacgga ttcaagttgt agtgttacga gcggaggtac
     3961 cctgagcttt gccgcgggag tggcttcgtt ttatatcaaa ggaaccactg ccggatgggt
     4021 taaccttcaa gccagtgcga caatagatac actcaatttt gtggcgaatt atggaatggc
     4081 aatttcctcg gcgggtcaac ccacacattt agttctggga actccttcgc cgagttattt
     4141 atatatcaat caatgtatgc ccatcacggt aaccgctaac gatgatacca atgcggaggc
     4201 gccgatgcca accactcaag tcatcaagtt cactgcgagt ggtggaggag caggaaataa
     4261 tttttatacc gacgccaact gtacgaattc gatggttaat gcggagactg gcattaccag
     4321 cggtcaaacg agtattaata tttattttaa accgcaaaat tctggatccc tgggcttgag
     4381 cggctttatc gacaatatga attattctat cggtggaagc caaaactata ctattggcag
     4441 tttgtggatg catgtgattt ccggtggctt caatggaagc tggtttgaca atacctgcgg
     4501 ctccttgact tttgaactga tggataattc tatcgcgggc tcggggaatg tgattcctaa
     4561 tctcagcggt tctcctgtgc ccatcaatat caccacgaat gcttcaactg tgagtggggg
     4621 actttattct gtcgccggat gcaccgattc cgttgctgcg tcgaagagta cggcaatcgg
     4681 ttcaggcgcc agcacttctg ttatttatat gaacagtttg ttgtcttccg ggtcttatgt
     4741 gtcggtcact ccctcttact ctggctcata ttcacccacc taccagaatg gaaatacaat
     4801 agcatcgacg agcttcgctt ggtgtccgcc tgtggatggt ggtggggctt gtgcctcttc
     4861 tctggccctt tccggaccaa acgtagccgg tggagtgggt ttatttacgc ctggaatcac
     4921 tgttggctcc tccatggata aaaccattac cattaccaat acttcaagca gtggtgtgag
     4981 cttggggacg atgacaatca ctggaggtca ggctgctgaa tacactttca aagggggaac
     5041 ctatccaggc acgggtggaa cttgcacggg ttatatatcg ccatccggca cttgcactgt
     5101 ggtagtgact tttactcctg cagcaataaa cggacgctat gcctctttga atgtcaatta
     5161 caacggcact tctttgccat tatcgctgca agggacgggg ttataggcgc atcgaattta
     5221 ttcaaactgt tatttaactc agctgggact gtctggcgac aaagtctctt ttcttgataa
     5281 gctcatgcgc cagtaaagta actttacaga attgctgaca ctggtctaat ctttgagctg
     5341 gcctttttag ttcacttgaa aggttcgtga tacctatgag ttcagcaatg tggttcgcgc
     5401 tagcgggcgc tttactgata gtgatggccg tgatctcagc ggcgctcaag cgacttcctc
     5461 tgacaaccgc catcgtttat ctgacgatag gaattttatt gggggattct ctcttaggcg
     5521 ttatttccct ggatttactg cgtgacgtga agctgttgag gcttctcacc gaaattgcgg
     5581 tgatcatctc cctttttact gcgggtctta aattgcggct tccgctggac tccatggagt
     5641 ggcgggttgc tttttggctt gccacctttt ccatgctcat caccattttc cttatgacgt
     5701 tatttgccca tcttgttttc ggcttttcag tgggagcggc gattctcttg agtgccatcc
     5761 tagcacccac tgaccctgtt ctggcttcga gtgtgcaagt cctgggtcct gaagaccagg
     5821 atcgtttgcg ctttagctta acagcggaag cgggtctgaa tgatgggatt gcatttccct
     5881 ttgtgatgtt aggcctggga cttcttggat taagagatct tggcgagtgg ggcctgcgtt
     5941 ggttttcaat cgatttgctt tggggggtta gcggtggaat tctcattgga actttactag
     6001 gcgctggcgt gggcaaagtt gtcggctatc tccgagccaa acatgaagag gctgaaagtt
     6061 ttgatgattt cttagcactt ggcttaatcg gcttgtctta tggggtcgca gtgcttttgc
     6121 atgcctatgg ttttcttgcc gtatttgcgg cgggcttcgc gattcgccag cgcgagcggc
     6181 aagagagtcg aagttttcgg ccgatgctcg ataaatccga agggcttttg aggacggaca
     6241 ccgtctcggg acatatggct cttggggttc tggaatttaa tgaacaaatg gaacggatcg
     6301 gggaacttat tgtggtgaca gctctaggcg ccctcctctc tagagaaaat ctgagattgg
     6361 aatatttctg gtttatacca gtattgtttt ttattgtacg ccctatttca gtattaatag
     6421 ggacaggtct taataaaggc ggtcataaga ttttaatctc ttggtttgga attcgcggca
     6481 tcgggtcttt atactattta gcttttgtaa ttgaacaagg gcttccggcg gagcttgaaa
     6541 aaaaaataag cgcgatcact ttttcaatgg tggcggcctc catcctagtg catggtttgt
     6601 cctcgacccc tttgatgata cggtatgctc gaggcaatcg caggtagaag tggaaatctt
     6661 gaaaaacaat atcggacctt aaatttcgct atgttctggc agaggttgaa tggattcggc
     6721 atgggcttta tcgatacctg taaaactcat tatgagaaaa gccgaaagta gaattagaat
     6781 aaatccttca atttgagata cgttttgtga tttcatttaa ttctcctata tagacatatt
     6841 aagaatgcaa aactcatact cgcccattgg aatagggagt gattggacaa ccgggggttg
     6901 atgaacaatt agctaccctg tcgctttgga acgagacgct tttccataag gagtctgttg
     6961 cataaaatta tagaattaca ttcatctatt ttgggaattt gcagaggtga aaatactcta
     7021 aacaaccgcc ttaatgttag ttaatccaaa ttaagatagg cactttttga gacggtcgct
     7081 attagagaca ttggatgttc gcgtgagacg gagttaaagg agccttaatc tcaaccacaa
     7141 aaggaattgt ttgtacttga tcatgatgct tgaattgcac ccaaattttg aaaatccctt
     7201 ccgtcatagg ggcgtggcta tcgctggcca ctctgaggta aggacctcgt tctgtttcag
     7261 gatcatcaac aagaggccat accgcatgca ggtggatgaa ttttttttct ttggcgatgg
     7321 ttccttgctc agaaagcatg atcgtgtgcc caaaagagct gagccagggc tgtaaatctg
     7381 tcacgtccag ataagtttgg ctgagaggat ctaaatgttt gagttccatt tcgagcaaag
     7441 cggcaaagtt gccgcagtgg ggatatgtat tcactttaag cttcacctga taatcattaa
     7501 aatttttata aatgaatcca tccgatgaaa cgggatctat tttgagcggt tttttctgag
     7561 ttgcaactcc ggaggcccta aaatccagtg gcagggtggt cattgagaaa cccatgggca
     7621 tggactctgc aaatagaaag taggggccgg gtcttttaac agcgcttatg gcatccagat
     7681 tgtaaggatc ctgagtcggc tggttgagat caattccaaa gatcccaatg tgatcactga
     7741 ggtgttgggg atggagatgg gcaaaggact ctaaatcctc gctgattaaa atcaaatgca
     7801 tttctttttc atgcatggga tgaaaatgat gaacggggtc cggcgagtcc gaattgaaga
     7861 cttcaatttt cagattggtg gcttcgccgg cggtcatcac tgggttttct tcgttattga
     7921 aaaacagttg agctgccagt gctgcaaagc accggctcgt taaagcgagg ctcaaaaaaa
     7981 gcgcaaaact caaagacgcg atcttcatcg gaccgcttcc ctggcgcctt caaaatccac
     8041 gacaatgagt ccgttctcga tgtcggaacc gatgacttta ccagacttaa agaaaggata
     8101 acaagcccac atgccgacaa atcccttttc aacttcctcg cttcgagtcc aaaaagccac
     8161 ttcattcgga tgaagtggat tgctcaggtc caggatacga atgccgccac cataagagga
     8221 gaaataagcg taatttcctt tgatctgtac attgtgcgcc aatcgattcg gtggaagcca
     8281 ctctgcaatg accttgatcg cattcaaatc actaatatcc catattttta ccgtgcgatc
     8341 ttgagtttcc tcggtcgtga ccagatagtg ctcatcttct gtggtccaag aattatggaa
     8401 agaaactgca ggtgcatcct tgtcgcggac gcgtttcaag aatttaggat tggccgtatc
     8461 agcaatatcc caaattgaaa atgtttggga catgatttca ctcaaatagg ctcggccatt
     8521 ttttatgtac gcatcatgga cataacttga accaaagcgc gcaatttctt taggtttaag
     8581 cgggtcctcg aggcttaaaa taaccactgt ttctcccgat ccgcccatga gatacatgag
     8641 ctttcgcggt tcatcaatag tgatggtatg gctcataggc aggtctttat aaacactcac
     8701 caaggtggcc gagctaggaa gttgcgcaag atcaatgatt tcaatacctg tatcggcgtt
     8761 gtcagtgact acgtaagcat aatgagaata agttttcatc tcacgccaca agcttcttac
     8821 gctctttatg aaagccactt ctttcacatt attattgtcg gtgatgtcaa taatagccgt
     8881 tccggttcga actcccaaga tggcatattc tcttccatcc ggagcggtgt agccccagag
     8941 agctgaatat ttatcatttg catgttcgtt cacctgagca atgaaattca ttccttgaat
     9001 ttgtggcgat aatgcataga tctgatgaat gggcacaatt gtaagacaaa acaggacgaa
     9061 aacgcttctg acaaacctca tcatgaaccc cccttggctg atgatggaat cacggtaaca
     9121 agaagacaag gaatctgtaa aacattttgt tggagttcag tcgaaaagat tttttcgaaa
     9181 tagacgtagt cgacgctaaa gtaaggttaa caaactactt atgattgata gagatgctat
     9241 tatatttccg aaattgtcaa cgacgaagtt gcagattttt tgttcaactc gccactcgcg
     9301 tcttgaatga taggttttgt tgatatatcc agtttaaaaa cgggaggaaa aatgaaaatg
     9361 aaatttacct gtatggcagc tttattagga tttgcgacta cgagctgggc tggcttaccg
     9421 acggaggcct tcggacgcta tcatgtcgtt tcctacgtgg aaaatggcag aacgattccc
     9481 atgaaagatt ccggcgcatt agagaacgct tcagctgaag tcaaaaaccg caccgaggtt
     9541 tataattttt ctttcaacac cagcgatggc cacacgtttt cggtggatac cctgggaatc
     9601 tatgaggctg ccttttatgg ggttactgcc gacgctatcg aaattgtcaa aaatgaagtt
     9661 aaaacgaaag agctgactac agatttggat tctctgatcc gcttcggtaa atgcgaaagg
     9721 ataacgaaaa cacatttcgc ttacgactcg gcattttctt ggatcaagat tacctcgtac
     9781 atgaaacttt gcaatggcaa agagtatgaa cgagagcttt tgatgaataa atagcctacg
     9841 gagtgtcatt agttttcagt attggcaaca ttcgaactcg gcttaggggt ggcgcaaata
     9901 ttttcagtcg ttgctaaatc atgagtgatg gcgtcaacca gggcatgaag gagcacgtct
     9961 cgtgggaagg gattggcaat tggttttttc tgagcatcca cattgacgaa ggcataagga
    10021 agggtcaatc cccagtggtg agttctggca acacccacaa gtttgctgtc aaaacaatgc
    10081 ccgttggtca attcacgagc gacactcggc cacagaaacg ttttgtacag atcgacctgg
    10141 ctgtcattga tgcgtgaccc cgcaaattca aaagggagcc cgtcggtttt tccaacattg
    10201 aacaattcac ggtggttgaa agtaaggaag cggtcatcga cagtattcag gcgccatccg
    10261 tatccaattc ctttttgaaa aaaagtttga ttgggttgat caagcacgcc gccaatgttg
    10321 taatacttta tatgggaggg aagtgaaggc cctttggttt tccaccactc cagtcgcgcg
    10381 ctcgttccta tctcggaagc gccttcccaa gctctgttaa agtaaaagaa aaacttggcg
    10441 agattgagat taaattcttt taaaatagcg gcattcaact ctggcatgcc atgaagggct
    10501 gctgctgtaa ttctttgcgc ggattcgtcg gctccccagt ttttcataag ctgagcattt
    10561 aaagcgagag cggcttgagg atcaggaata atttcccgga gggttgtgaa tttatttttc
    10621 cagttcaatg ggagttgttt caagatttct aaaaaaggtt cgtttgaata attcgtgggt
    10681 taaaatttaa gcaaattatg ttttttttga aaagctgaag gtcttcgctc agcagtgagt
    10741 gatattgcga agtttaggct ctacatcaat aacgggggca gtcaggattt gggtttaaat
    10801 tcggatttgg gcagagagtt tatccgcgtc agcggataat aaagagaacg tcattgagtt
    10861 aagcaaacac cggcagcttc cccctgaggg gaaagtcgct gagtctgatt ttcataatat
    10921 gggcatcgaa aaattgcagg ttgaagaatt tggtagtgag aggcgttatt gaaccgggca
    10981 cccaagttgc aacctatcga gaaatcgcct gaaagtaaac tgaaaaatgt gagaaaaatc
    11041 atagacttgt ggaatctcaa gagagttcta tcaactgaaa atgggttggt aaaccgactc
    11101 aggaaataag cgatggcttt atatggtaca catttgggta cttgtgctat atttgcttta
    11161 agtgagaggt gtttatggaa tcgacttcgc ccaggagctt ccgagctaac ttgaaggaat
    11221 acatggagat ggctgatgag aatcctattc gcatccagaa gcgcgagggc aatgcctgtg
    11281 tgctaatgtc agaggagtat tatgaggcct attgaaagag atttcgtctc tccagaagag
    11341 tctcctgtcc gccaatcagg tgctcaaagg agatgtcact gaatacaaga ttggcgacac
    11401 agatcgtctg caaagattta aaaaatgaag attgtccgaa ctctacagtt caatctaatt
    11461 tattcatgtt atgttgacct tggggagctg aaatgaccga tgcaaatgat gttttgaaaa
    11521 aaagaatcct gagtcagttc caagtaagtg aagaggatct gataaaactt tgtcaggcta
    11581 tgggcgtcaa gtctttggct atattcggtt ccgcgcttca gggaaatttt aagcccggtc
    11641 aaagcgatat cgacttcctt gttgagtttg aattggtctc catcgattgt ttctttgatt
    11701 ttctcgaagg tctgaaaaga ctttttcact acactgacat tgatctggtg actcgaggct
    11761 ccctgaagaa tcaggtgatt cgcgaagaga ttctgtcatc caaaaaggat ctttatgccg
    11821 cgtaagttag aggcttatgt ggaggaaatt ttagagtcta tagctcagat cgagtccttc
    11881 gtcagaggaa tcgaattcgc ggaatattta caagacgacc gcacaaaagc ggccgttgaa
    11941 agaaagcttt tgaatattgg ggaggctctc aatcaggcga gtcaagtcag tcaagagata
    12001 gaaacaaagg tgactgaatt tagaaaaatc gtcggattca gaaatgtttt agttcatggt
    12061 tattttgggg tcgacgatgc ccttgtttgg gatatcattg cgaccaagtt gaagcgtctc
    12121 aaatctgatg ttgaatccct atgaatgaag gaacgaccga aacattagca gacattggaa
    12181 gaatccatgt tcccgaagta gcgaatcttg atgaagagcg aaggaagcga gaagagaaaa
    12241 aaggttcgta aaataaaggg gcttttcagc ccctcagaaa taaaatccta cttacagtaa
    12301 ggaacgaaga tagtccaacg gttagcatct tgcttcatat tcataaatga catgaactga
    12361 ttatcgttaa tgtcagattt attgaatgtt aaatctgcgg aacaatattt tccatcaccg
    12421 aaagaatctc taaaagtgat tttaacaact tgatagcccg ataagctcaa agccgttaaa
    12481 tcgtacattg taccacttcg ttgagcgaca ccttcctttt caagctgaat taagtttgaa
    12541 agaacagaaa cgatatcact ttgtttagtg ataacattat acacttttct gtcttcggcg
    12601 tttgcgaagg cagaagctaa caatacagta gccagaatca gtttcttcat agagatttct
    12661 ccttgtttaa gttttaaaca cttattaagt ttaaggctca aaaatggctc ttcctcacca
    12721 tgagaaagaa ttaaaccatc aatgaaatcc acatagactc tatatgcgaa aatcaatcca
    12781 gatttcccat ttcgggacag ttttcctgaa tttcaaccat gtcagattca atctgtgcga
    12841 tgggaacaag tggaaaaagc tgaatcatct cttcaagggt tcgttccaga cattcaattt
    12901 cgtatcgggt caaaaaacag tatttatcca tcttccctcc ataggtaaaa accaaaattc
    12961 ttttctcttt gaaacagaat ccgagccaga gtttcaaggt cagcctccca gccatcaatt
    13021 aaaagcctcc cctctttgat ataggaccga gcaatgaagt gggcttgtga aggttccttt
    13081 ggctgtggcg
//
LOCUS       DATBTE010000258         1890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_718048, whole genome shotgun sequence.
ACCESSION   DATBTE010000258 DATBTE010000000
VERSION     DATBTE010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718048"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VIG33_09600"
     CDS             complement(<1..1194)
                     /locus_tag="VIG33_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271128.1"
                     /translation="METFQRSFPQDPGKFCTLFTIPRLRVEFPYLDGTLDKYQVERVI
                     KEKIKSDLRLDLGKRGSEIGMISLDDIESKNMLIAQNDAVQQQNHKFYPKGVPTDEMN
                     GGTLRFSNGVFHEHYSLLREQLQSIKESGKFGFNVTHLLPMDALCVRARIDHECGGMM
                     RNIGVGRGKKSTVYEVEYLVNIRAIDGGQGTPLIEAGIISAELSSLSKALRYGYPFGV
                     SELPGAAKLEFSREWGTMIRQVVINNPFSEEERSQTVLLERVSNFWILRTPIFHELDF
                     NFQLPDVGEFVKGLNEDRDWGYLISDQKIIWFVAFILDGESVDRCVDFIEYVAEKVGK
                     VGDRLQHLYRGMDEKDCWGYRSRAGFPSLPDFSEDTKRRVSSYKVDLTKMNQQELQQC
                     GRLIYG"
     gene            1247..>1890
                     /gene="istA"
                     /locus_tag="VIG33_09605"
     CDS             1247..>1890
                     /gene="istA"
                     /locus_tag="VIG33_09605"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEY8271129.1"
                     /translation="MDVIAEIRRRHHIDKESISSLASTFNLSRPTIRKHLKTIEEPVY
                     QRQNQPHPKLGSFHQQLDAWLEQEADLPRKQRRTAQRLYECLQVEGYLGSYTVVQRHV
                     KRWKKTRAGSPSVKQAFVPLAFPAGETCQFDWSQETVLLGGVVQTIKVAHFRLAYSRQ
                     MFVVAYPRETQEMVLDAHSQAFAYFGGVPKRMVYDNLKTVVDAIFVGKERRFNRR"
BASE COUNT          482 a          491 c          413 g          504 t
ORIGIN      
        1 cccgtaaatc aatcgaccgc actgttgtaa ttcttgttgg ttcatttttg ttaagtccac
       61 tttgtaagat gacactcttc gctttgtgtc ttcgctaaaa tctggcaagg acggaaatcc
      121 tgcccttgat ctatatcccc agcaatcctt ctcatccatc cctctatata aatgttgtag
      181 acgatcaccg acttttccaa ctttttccgc cacgtactcg ataaaatcga cacaccgatc
      241 gaccgactcg ccatcaagta taaatgcaac aaaccagatt attttctggt cagaaatgag
      301 atatccccaa tccctatcct cattaaggcc ctttacaaat tctcccacat caggaagctg
      361 gaaattgaag tcaagttcat gaaatattgg ggttcgcaag atccagaaat ttgacacccg
      421 ttcaagcaga acggtctgag atctctcttc ttcagaaaac ggattgttga ttaccacctg
      481 tctaatcatg gttccccact cccggctgaa ctccaatttg gcagctccag gcaattctga
      541 gacaccaaat ggataaccat atcgaagcgc tttagatagc gatgaaagtt cagcagaaat
      601 aatcccagcc tctattaaag gagtcccctg acctccatct atcgctctga tgttgaccag
      661 atactcgact tcatacactg tcgatttctt acccctgcct accccaatat tgcgcatcat
      721 tcccccacat tcatggtcaa ttctggcacg cacgcacaat gcgtccatgg gcagcaaatg
      781 agtaacattg aagccaaact tgccggattc tttgatgctt tgaagctgct ctcgaaggag
      841 actgtaatgt tcgtggaaca caccattgct aaatctcaat gtcccgccat tcatttcatc
      901 agttggaaca cctttaggat agaatttgtg attctgttgc tgcactgcat cgttttgtgc
      961 aataagcatg ttctttgact cgatatcgtc gaggctgatc atcccaatct cactgccgcg
     1021 cttgccaaga tcgaggcgca gatcgctttt gatcttttcc ttgatgactc gctcgacctg
     1081 atatttgtcc agcgtaccat ccagatacgg gaattcaacg cgcagcctcg gaatggtaaa
     1141 aagcgtgcaa aactttccag ggtcctgggg aaaagagcgt tgaaaagttt ccacctcagg
     1201 ttatttaatg tccggttttt gaccggagaa tcctggagtg ttagcaatgg acgttattgc
     1261 cgaaatcaga cggcgtcatc atatagataa agaaagcatc agttctctgg cgagtacctt
     1321 taatctgtcc cgccccacca tccgtaaaca tctcaaaacg attgaagagc ctgtttatca
     1381 gcgtcaaaat caaccccacc cgaagttggg ttcgtttcat cagcaattag acgcctggct
     1441 tgaacaagaa gccgatctcc ctcgtaagca aaggcgtacg gctcaacggt tgtatgaatg
     1501 tctccaggtt gaggggtatc tcgggtctta tacggtcgta caacgtcacg tgaagcgttg
     1561 gaagaaaacg cgagccggta gtccctctgt caaacaagcc tttgtgccgc tagcctttcc
     1621 cgcgggcgag acctgccagt ttgattggag ccaagaaacg gtcttattgg gcggcgtggt
     1681 tcagactatt aaagtggccc acttccgctt ggcgtatagc cgtcagatgt tcgtggtggc
     1741 ctatccgcgg gagacccagg aaatggtgtt ggatgcgcat agccaagcct ttgcttactt
     1801 tggcggcgtc cctaagcgca tggtgtacga caacctaaag accgtcgtgg acgcgatctt
     1861 cgtgggcaaa gagcggcgct tcaatcggcg
//
LOCUS       DATBTE010000259        19073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1674037, whole genome shotgun sequence.
ACCESSION   DATBTE010000259 DATBTE010000000
VERSION     DATBTE010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 19073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19073
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674037"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VIG33_09610"
     CDS             complement(<1..472)
                     /locus_tag="VIG33_09610"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEY8271130.1"
                     /translation="MGLTKQEEETIYLIAEKITGTSQQGVFRKDILVRNVERRMVEVN
                     TRSLADYLQLVNSDPLQYQNLVSDLTIHTTSWFREKPHYDLLKQQAADLFKTAKRPFK
                     VWSSACSTGEEVYSAALVLEGLREHLQGFEYEIHGSDIDIVSVDKAQRALYEAAG"
     gene            complement(504..2189)
                     /locus_tag="VIG33_09615"
     CDS             complement(504..2189)
                     /locus_tag="VIG33_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8271131.1"
                     /translation="MTNKVFSLKVRLFLLTFALLAAQGVLIAVAITKSRSILGSLSKV
                     TEVSLPATKFLLNADMYHDGIRSSVFEISSLDPHSQKDKILKAEEELKEKTQEIASNI
                     KMLKAIHFPKSIKQDIEELDKNVSLYVEASFNLAESVANGKKENQEAFADQFHTEFSK
                     LEKMFQELEVRIENDIKSTDGQGKDIVKSVVIYSLFGLFLSSIVGLVIFIWTKKSFDQ
                     LLRKIADVSEEVSHLSIVVGDESGKVKDSTVEQSAAIQESVSALSEMSSMIAQTGQNI
                     KLSLETSQDALGKSTEGKQIMDRMSHSMASIQKANDQLQEISNVIEDIHRKTAIINDI
                     VFKTQLLSFNASIEAARAGQHGRGFAVVAEEVGNLAELSGQAAKDIEILLGDSQRKVR
                     ETLEMIQGRVSDGNKVSQSAMQAFNEISKNIDEINSQVRSINEATQQQEIGIQQTNLA
                     MKQMDIISQNNTVASTEALQIVQNLKSQSTDLREVMTALFELVGSRPVMKSSDHEKSS
                     RQESHSFKDSSAAKTDQHMESLIHQITMKKSVSAPVKESQSISADDPDFKRAG"
     gene            complement(2226..2675)
                     /locus_tag="VIG33_09620"
     CDS             complement(2226..2675)
                     /locus_tag="VIG33_09620"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY8271132.1"
                     /translation="MSLNENDYKYLVYKIGNELYSSPLLSVREVLEYQNPKYMPNMVH
                     HFSGVINVRGAIVGVLDLRLKFGLPNPVGPKTSILLCDTGRGLIAAVVDSVECVQEFA
                     ETDVDRKPVVMSKVKQEYLAGVAKSKEQLVTILDLHKFLTEEELKAA"
     gene            complement(2672..4390)
                     /locus_tag="VIG33_09625"
     CDS             complement(2672..4390)
                     /locus_tag="VIG33_09625"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEY8271133.1"
                     /translation="MDFDMEMITSFCDEALDSLTRWEQVCLELSQGENQDLYQELFRL
                     AHNIKGGSRAVGLIAMGDYVHQVEDGITLLRDGQLKLSEKIIQFLLESHKHLSEWIIE
                     AKTEPAYLPDHQTFLNEFKSFFQQLNAVNSGVSSETSTESRTLSSGVHPEAREMPQPE
                     TQTTAKTAVNEKRKAPSNETIRVSAQKLDQLIQVIGELSIHQTIVWHTRGGQENNNKI
                     FTNSLQLSQKLTKELYDRALSLRMQPLQSVFQRLERNIMDLSRSLEKAVKVEISGGEV
                     ELDKTVIEKIVDPLMHIVRNAVDHGIERPADRIMDDKPEFGTVAIHARQDTFGVELTI
                     KDNGKGLSSQNIRAKALEKGLISENKKLSEKEIFGLILLPGFSTAEKITDVSGRGVGM
                     DVVKRTLDDLNGSIEIFSEVGKGTQFTVTLPTSVSIIDALLITLSGHNYVLPVGAIEE
                     VINFEEGEMDLSKKMIVHNDHVMPLQDLESLLVAKASRRPTEKTQKKAILVCKHESNK
                     IGFLIDRVVGQQQVVIRPLNENINGSFGLLGGTILGNGEPGLIIDMSALASHFANEFR
                     TKERVA"
     gene            complement(4401..5813)
                     /locus_tag="VIG33_09630"
     CDS             complement(4401..5813)
                     /locus_tag="VIG33_09630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY8271134.1"
                     /translation="MKSNQILENLESPFGLAELFISRTDERGVIQFGNDVFVRVSEFS
                     KEELIGSPHNIIRHPHMPKAVFRLLWDTIKQGKIIAAYVKNRSATGKYYWVLATVFPV
                     KGGYFSIRLKPTSGVLQVVEDIYKEVLKVENEKGIDAALAHMVQLVKKAGFENYESFQ
                     AYALKTELQLRDQSLIPTSGSQKTHCCDTSQTRGFCEMHQLVLVAAHGFAETVKSLEV
                     FKDLKQKCGSESKVIARACERLENLAINMSISAHKLGKDGSTLAVVANTFQGTTKQII
                     QSYANFEKFSAAVLSKLEEVLFSISCSRVQTEMLAFFLKESIDGMSAGKISSEAEIRK
                     LLSELRVIILAVKDLFMVSSQQQNQFYEILLRLRKDTTELSKVIVRLDLIKTGGMLEG
                     SRSVDITSMFKHFLVEMGQLIGFINTPVNDILGALEASTGAFEKVILTIMHTEHVLGE
                     LELLQKNAVELLNATNMEAA"
     gene            complement(5803..5997)
                     /locus_tag="VIG33_09635"
     CDS             complement(5803..5997)
                     /locus_tag="VIG33_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271135.1"
                     /translation="MIAKDKLLLLRIINARGAKETSLLILNVSFHSISYIPGLITSLA
                     YTEEMLKFHEEFSTGSIDEK"
     gene            6131..6727
                     /locus_tag="VIG33_09640"
     CDS             6131..6727
                     /locus_tag="VIG33_09640"
                     /inference="COORDINATES: protein motif:HMM:NF022664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3011 domain-containing protein"
                     /protein_id="HEY8271136.1"
                     /translation="MTLFRLIPAACLLALSMVSAGQAQVGRIDPGGRVVITCSSSGYA
                     YNTCYVGQQVAYARLVSQLSRSACIEGSTWGYANDSIWVDKGCRANFEVITADYGRPP
                     GGGGYPPPGRGGYPPPGRGGYPPPHHPPIYEESINCASSGYRFNSCYPASGLRIRSAQ
                     LSRQDSRTQCVEGNTWGYDNNRIWVDKGCRGEFRVQTY"
     gene            complement(6767..7564)
                     /locus_tag="VIG33_09645"
     CDS             complement(6767..7564)
                     /locus_tag="VIG33_09645"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY8271137.1"
                     /translation="MKILLASLVLIFQSYQVFAYSIGTYNLHNMSDLEGLKRDLKNLN
                     HISIFAFQEVRFRDSDQDNLLLHGILPVGKYPYVVTQKVAKDDDGNIEGHAIVSKYPI
                     LKSMVIPLEHTDKKNREALVAWIQIEGREIMVINTDHEVHYKDISYLDRRKQILSLIT
                     ALDKINFKGTQILLGDFNTSDSIANWGSGVTGEKEVKLTYDFFKDRGWQPTYASSEKD
                     YTFQSWGFTQHLDHILFKNSGTDKIKPWLRFNERKGSDHYPIYSFFE"
     gene            7821..8672
                     /locus_tag="VIG33_09650"
     CDS             7821..8672
                     /locus_tag="VIG33_09650"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEY8271138.1"
                     /translation="MKKILALFLVFSLAPLKSWTEETIQDRLRNKKLVAAHQGGVFDG
                     TPNTLQLFEHARRNGADIIEMDLHLSKEGIPVVFHDDTLDSLTDCQGKVSDLTVAELK
                     KCEISFVFRGGNIPTFEEVLQWNNGRIILNAEFKVPEAVVPAIRLVEKYKAYSSVYFQ
                     TKGDPQLYTSARSQNPKVALLFAPPNVESLNWALSLNDDFLVVIELHENLRTKEIIDA
                     IHRAHKWASENSWHWRFDHEFFGVSCAEGFYEIGLDILVSNRPKQCGADRADFRISLK
                     AKDYNPR"
     gene            complement(8640..10250)
                     /locus_tag="VIG33_09655"
     CDS             complement(8640..10250)
                     /locus_tag="VIG33_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY8271139.1"
                     /translation="MPTRKKILAVVVFIAFILSIEIAFKSYARQVEVNVVPGDWPGFN
                     GTLDSQRFSPLAEIDQNNIGRLKVRCIFDLKEKLNFQNGPVVSQSVMYITTENDTYAI
                     DAATCKQIWKYHREFKTATNSLHVNRGAFFANEHVYRGSNDGYLIALNAKTGALLWET
                     KVANPDVGESLPAVPVVWNGIVYIGQAGGDNVGVRGRLMAFQESDGKKLWSFELAPIT
                     GPGSETWPLSSVQVPRTGAATWTSYTIDEANGFILIPVGNAAPDFVKSVRPGLNLYSD
                     SVVILDAFTGTLKSWYQLTKDDIHDWDVSTVPVLYRASNGHLMMAEAGKDGYLYFVDL
                     DIKSEILAKPTTSHLNEEVPMTVSGTRFCPGTQGGSEWNGAAYHPTLNTLFVPATDWC
                     TTVKLAESGLPAKAIPGEPFTGADPREPFGTMDPKSNWRGWLTAFDANDGNVRWQYQA
                     ATPLIAAVTPTAGGLVFTGDLDGNFLALNAETGKVLYQSKIGEPIGGGVISYETNGKQ
                     LIAVAAGLQSRTWKTQTHSSRIIVFGLE"
     gene            10497..11225
                     /locus_tag="VIG33_09660"
     CDS             10497..11225
                     /locus_tag="VIG33_09660"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEY8271140.1"
                     /translation="MLRLLYRLFYSVAFILGFSFHSLASSPLLPFVNGGDKVLADDPI
                     AKSTVMLETDKQYCSATIISDNMLLTAAHCIGENEPWVLIHFSGLESALTRSASRSLR
                     HEGYQDLQDTTRNDIALVFFEGGLPEGFLPMMILPADKDLAVGDELQIAGYGAGGPLG
                     SLAKVSLKVSDFLDTRRLIKFAQTTHRGICHGDSGGPAFKVLNNQLYLAGVASYANEI
                     DCSDYSVYTRATNYIDWILQKQKE"
     gene            11393..12376
                     /locus_tag="VIG33_09665"
     CDS             11393..12376
                     /locus_tag="VIG33_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8271141.1"
                     /translation="MIKKIFSLLLVISLNACFAQASSDLQIHFLSIPYGSEVLNLRIG
                     IQNPDQVAVGDILYIHGFGDRLDNHQPLFRAWTKAGFRVIAFDLPSHGENSGTYNDLN
                     NFSFEELAALAVKVEGETKPDTARPLILSGWSTGGLVVVRMIQEKWMADLSRPVSGVI
                     LFAPGISVRKFPWTFGNRLGMVTEKTLTHDPHPPHVAPVRPDTPFWSHLAFQFSPRLV
                     AESILSQHRSYPTDIPTLIFTGGDAEDMYAKEWVVRSWIQEQNRVRQSSQSSPIVNVA
                     CSGARHELDNETESFGGLEVQNSSAAFAQAIAAGKLEMFSAGKDTFGKICK"
     gene            12525..13082
                     /locus_tag="VIG33_09670"
     CDS             12525..13082
                     /locus_tag="VIG33_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY8271142.1"
                     /translation="MEIYEILKNDHQEVKDLFEDIDEFLRNGNFEEAESAFETLRVEL
                     TAHSKAEQEVFYQPLKVISEDLEDDEVSGEELLGEAEQEHHLVALLLNELSRLDVEEE
                     DWKAKLTVLQELVDHHIETEEGEIFDEARKCFSAEEAEMIAENMQDLKEKYKGMIESA
                     LAEDIALFNQPIRNPVDASQTSFSR"
     gene            complement(13093..14163)
                     /gene="galK"
                     /locus_tag="VIG33_09675"
     CDS             complement(13093..14163)
                     /gene="galK"
                     /locus_tag="VIG33_09675"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEY8271143.1"
                     /translation="MKNITAQAHGRVNLIGEHTDYNGGWVLPTTIPQKTLVSLWRRED
                     RRVSASSSNLEEGGGREIYYSLGEEAPTKTWIDYIQGVTRILDQEGMKISGFKLQVDS
                     TVPVGSGLSSSAALEVSFLKALREAFSLNLSDIEIAKIGQRAENEFVGARVGIMDQMA
                     CCLAKEGEALFLDTKTLAYQHIPLCLDKMDLLVINSGVMHDHAGGDYNQRRSECEEAC
                     GRLKIHELRELTLADLPKLEELPPVLQRRARHVITENQRVHSAVQALLKGEVVRLGEL
                     FYESHLSMAQDYEVSIPEIDTLVRLCREHGDVYGARLTGGGFGGSIVGLTRPGTSKTV
                     GSFVARQYHELTGLQAKVLVSS"
     gene            complement(14160..15245)
                     /locus_tag="VIG33_09680"
     CDS             complement(14160..15245)
                     /locus_tag="VIG33_09680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY8271144.1"
                     /translation="MTEAAIKINKLREQMVSEKTQTLRLKGVDWFSWATCGGRSTVIF
                     TSELGVAEIFITATKAWVLTNKIEQDRLAQEEVPLEFEIMAFPWEQASLLEKFIQQHS
                     PGACYSDRPSGSEKLIPDSFRDLKLILGPEEIQRYRQVGRLAAEAMTEALTKAEPDWS
                     ENQLAGEGARALWTRGLDPALVMVGSHRRCLLHRHPIAGSEKLGESAMMVFCARGFGL
                     YANLTRFIYFRPLTSDEERRFAMTLAVEAAAFHSSVPGKTLTELFKEIAGAYRNLNCA
                     VEVERHHQGGPTGYLSREYVVSPESPPQVKLSRGMALAWNPSLPGAKTEDTVLMSENG
                     IEILTVDPHWPTTSFAERSRPQVWVKS"
     gene            complement(15245..16327)
                     /gene="galT"
                     /locus_tag="VIG33_09685"
     CDS             complement(15245..16327)
                     /gene="galT"
                     /locus_tag="VIG33_09685"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEY8271145.1"
                     /translation="MEKRDFIKPDGRGLTLYSRRPISPSILPTNPPHVEVMGTPHLRW
                     HPLRGEWVAYAAHRQNRTFLPPKEYSPLAVTRSREFPTELPAGDYDIAVFENLFPSLT
                     DRAQESPELSIPTKPGKGHCEVVVFTQDPHTSLGALPLDHIELLLQVWADRTRVIGSR
                     EEIQYVMPFENRGVEVGVTLHHPHGQIYCYPLVPPIPQRMFEMQSLYFEENQRGLLED
                     LVQDEVIKQNRLIHEEERVVSFIPPCARYPYETWIAPKRRSAWLYDLNNQELKDFAKT
                     LKTVLLKFDNLWNRTFPYLMVLYQAPTDGNVHPESHVHIEIFPPLRTKDRLKYLAGTE
                     LGAGFFINDTLPEEKAAELRAVEVTL"
     gene            complement(16299..17306)
                     /gene="galE"
                     /locus_tag="VIG33_09690"
     CDS             complement(16299..17306)
                     /gene="galE"
                     /locus_tag="VIG33_09690"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY8271146.1"
                     /translation="MRILITGGAGYIGSHAVRETLAQGHQVEVLDDLSNGRREAVDPK
                     AELIIGSTGNEELLLNILKTHHIDVVMHFAANIEVAESVADPEKYYQNNFVHSLCLLG
                     AMRKAQVLRLIFSSTAAVYGTPKKIPIEETQDRHPINPYGRSKMMTEMAIEDYSRAYG
                     LGYTILRYFNVAGAHPDGTIGEDHHPETHLIPRILAVALDTQNPVKIYGTDYPTPDGT
                     CLRDYVHVVDLARAHVLAIESTQPGHGEVFNLGSEKGFSVREVLRACEEATSLKLPVK
                     EEDRRPGDPPALVASSLKAREVLNWRPQYPEIQSIVKHAWQWHSLHPEGYNGKKRFYK
                     A"
     gene            complement(17348..18496)
                     /locus_tag="VIG33_09695"
     CDS             complement(17348..18496)
                     /locus_tag="VIG33_09695"
                     /inference="COORDINATES: protein motif:HMM:NF015247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEY8271147.1"
                     /translation="MKQKIGIAGAGFAGAVLARELAESGGYTVTVYDERSHVAGNCHT
                     FRDPQSGIMIHQYGPHIFNTSREEVWNYLNRWGKFGDFRNQVKAVTKRGVFSLPMNLL
                     TLNQFFGKKMNPREAHDFMLSQGDVSVDEPKNFEEQALKFLGKDLYKNFFYGYTKKQW
                     GVEPTELPASILKRLPVRFNYDDNYYNQKYQGIPVEGYTKIIENILDHEDIKVHLGKR
                     LPREQKNEFAHLFWSGPMDAYFNFDLGRLGYRTLKFERFDEHGDYQGNAVINYCAEEI
                     PYTRIAEHKHFAPWEEHAQTVCFREYSAFCTENDIPYYPLRLNSDKSLLEKYISLIEK
                     EDKITFIGRMGTYRYLDMHVVIGESLDLAKICLEKNILQWPKFSHHPL"
     gene            complement(18507..>19073)
                     /locus_tag="VIG33_09700"
     CDS             complement(18507..>19073)
                     /locus_tag="VIG33_09700"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEY8271148.1"
                     /translation="DGTLEIPYVESHKPSPLNVYGRSKAESEVKVLSLYSEALVVRTS
                     SFFGPWDQFNFATNILKDLSLKKKVFVCRDIKVSPTYIPDLVHTCLDLLLDGEYGLLH
                     LANEGALSWAEFARVVADQANQRNFCLDTCLDISLDTSLIIEKNLAELNLRAPRPRNS
                     VLTSEKIKVMPTFENAVSRYFSELEIRI"
BASE COUNT         5103 a         4486 c         4029 g         5455 t
ORIGIN      
        1 agcccgctgc ttcataaagc gcacgttgag ctttatcaac acttacaatg tcgatatcgc
       61 tcccgtggat ctcatattca aatccttgaa ggtgctctct gagaccttct aaaaccaatg
      121 cggcggaata aacttcttcg cccgttgagc aagccgatga ccaaacctta aagggccttt
      181 tcgcagtctt gaagagatcc gcagcctgct gttttaataa atcataatga ggtttttctc
      241 gaaaccaaga ggtggtgtga atggtcagat ctgaaaccag attttgatat tgaagaggat
      301 cactattcac caactgcaaa taatcggcca aagaccgcgt gttgacctcc accattctgc
      361 gttccacatt tcgaaccaaa atgtctttgc gaaacacccc ttgttgggac gttccagtta
      421 ttttttcagc aatcaaatag attgtctctt cttcttgctt ggtcaatccc attcgctacc
      481 ttatctttga ctctctagtt taactatccg gctcttttaa agtctggatc atcggctgaa
      541 atgctttgtg attccttaac aggggccgaa accgactttt tcatagtaat ttgatgaata
      601 agactttcca tgtgctgatc cgttttcgcg gcgctggaat ctttaaaaga atgggactcc
      661 tggcgcgaag acttttcatg atctgaagat ttcatcaccg ggcgactgcc aaccaactca
      721 aacaatgcag tcataacctc gcgcaagtcg gtactttgac ttttcaaatt ctgtacgatt
      781 tgcaaagctt cagtggaagc cacggtgtta ttttgcgaga taatatccat ctgcttcata
      841 gcgagattcg tctgctgaat gccaatttcc tgctgttgtg tggcttcatt gatggatctt
      901 acttgggaat ttatttcatc aatgtttttt gaaatctcat tgaaagcctg catcgcactc
      961 tggctgactt tattgccatc actgacccgg ccttgaatca tttccaaggt ttctcttact
     1021 ttccgctggc tgtcccctag aagaatttct atatcttttg cggcttggcc gctcagttcg
     1081 gccagatttc ccacttcttc ggcaacaaca gcgaagcctc ttccgtgctg accggctctc
     1141 gccgcttcta tggaagcatt aaaagacaat agctgggttt taaacacgat atcattaata
     1201 atggcggtct ttctatggat atcttcaata acattggaga tctcttgcag ctgatcattc
     1261 gctttttgaa tcgacgccat ggagtgtgac atgcggtcca taatttgttt tccttcggtg
     1321 gacttgccca acgcatcttg ggaagtctct aaagaaagct taatattttg accggtttgc
     1381 gcgatcatac tgctcatttc gcttaaagct gaaactgatt cctgaatcgc cgcagattgt
     1441 tccacggtgg agtctttaac tttacctgac tcgtccccca ctacgatact gagatgactg
     1501 acttcttcag aaacatcagc gatctttcgc aagagttgat cgaatgattt tttagtccaa
     1561 atgaaaatga caagtcccac gatggaactc aagaacaggc caaataatga gtagataaca
     1621 accgacttca cgatgtcttt accctgacca tcagtgcttt ttatgtcatt ttcgattcta
     1681 acttcgagtt cctggaacat tttttcgagc ttcgagaatt cagtgtgaaa ttgatcagcg
     1741 aaggcttcct gattctcctt ttttccgttg gccacagact ccgccaaatt gaagcttgct
     1801 tccacgtaaa ggctcacatt cttatcaagc tcttctatat cttgcttgat gcttttcgga
     1861 aaatggatgg ccttcaacat ttttatattg gaagcaattt cctgagtctt ttccttcaac
     1921 tcttcttcag cttttaagat cttatctttc tggctgtgcg ggtccaggct ggaaatctca
     1981 aatacggatg agcgaattcc atcatgatac atgtccgcat ttaacaaaaa tttcgttgcc
     2041 gggagggaaa cctctgtcac cttgcttaag ctcccgagaa tggatcttga cttggtgatt
     2101 gccactgcta tgagaactcc ttgagctgct aaaagtgcaa aagttaatag aaacaatctc
     2161 actttcaggc tgaatacttt atttgtcata attttctcca ataattattt tattaaattt
     2221 cataatcaag cagccttaag ttcttcttca gtcaagaact tgtgaagatc gaggatggta
     2281 acaagctgct ctttgctttt tgccacgcct gccaaatatt cctgctttac cttggacata
     2341 accaccggct ttctatcgac atccgtctcc gcaaactcct ggacacattc cacggagtcc
     2401 acaacggctg ctatcaaacc cctgccggta tcacatagta aaatagatgt ttttgggccg
     2461 actgggttcg gtaaaccgaa ttttaagcga agatcgagaa cgccgacgat ggccccacgg
     2521 acatttataa cgccactaaa gtgatgcacc atatttggca tatacttcgg attctggtac
     2581 tctagaacct ctcgcacaga aagcaatgga ctcgagtaaa gctcattgcc aattttgtag
     2641 actagatatt tatagtcatt ttcatttaaa ctcatgctac tctctctttc gtgcgaaatt
     2701 cgttggcaaa atgcgatgct aaagcggaca tatcaataat taaaccaggc tccccattgc
     2761 caagaatggt tccgcctaaa aggccgaagg agccattgat gttttcattt aaaggacgga
     2821 taaccacctg ctgctgtccg accactcgat caatcaaaaa accaattttg tttgattcat
     2881 gtttgcagac caatatagct tttttctggg tcttttctgt aggtcttcga gaggcctttg
     2941 ccactaacag agactctaaa tcttgcagcg gcattacatg atcattatga acaatcattt
     3001 ttttagataa atccatctcg ccttcctcaa agttgatgac ttcttctatg gcccccactg
     3061 gaagaacata attgtgtcct gacaaagtga tcaataaggc gtcaataata cttacggagg
     3121 ttggcaaagt gactgtaaat tgcgtgcctt tcccgacttc actgaaaatt tcaatactgc
     3181 cgttgagatc gtcgagcgtt cgcttcacta catccatccc aaccccacgg ccggaaacgt
     3241 cagtgatttt ctccgctgtc gaaaatcctg gcaataagat caatccgaaa atctcttttt
     3301 cgcttagttt tttattctca gaaatcagtc ccttttcaag ggccttggcc cgaatgtttt
     3361 gtgatgacaa gcctttgcca ttatctttga tcgttagttc gactccgaag gtatcttgtc
     3421 gggcatggat tgcaacggta ccgaactcgg gtttgtcatc cataatccgg tcggctggac
     3481 gctcaatccc atggtcaacg gcattgcgaa caatatgcat taagggatcc acaatcttct
     3541 caataaccgt cttatccagc tctacttcgc ctcctgaaat ctccactttg acagccttct
     3601 ctaatgatct ggaaagatcc atgatatttc tttccagacg ctgaaacacc gattgcaagg
     3661 gctgcatccg caatgacaag gcccggtcat aaagttcttt ggttaacttc tggcttaatt
     3721 gcaaagaatt ggtgaatatt ttattgttat tttcctggcc cccgcgagtg tgccagacaa
     3781 tcgtttgatg aatggaaagt tctcctatca cctggatcag ttgatctaat ttttgcgcgg
     3841 aaacgcggat ggtttcattc gatggggctt ttctcttttc gttcaccgcg gtttttgccg
     3901 ttgtttgagt ctccggctga ggcatttctc gcgcttcagg atgaactcca gaagagagcg
     3961 tccgagattc cgttgaagtt tccgaactaa cccctgaatt tacagcgttg agctgttgaa
     4021 aaaaagattt aaattcattt aaaaaggttt gatgatcagg aagataagcg ggctctgtct
     4081 tagcctcaat gatccattct gataaatgct tgtgagactc tagtaaaaac tgaatgattt
     4141 tctcgctcaa cttcaattgt ccgtcgcgca ataaggtgat tccatcttcc acttgatgga
     4201 cgtaatcccc catcgcaatg agacccacgg cacgagagcc gcccttgata ttgtgcgcca
     4261 acctaaagag ttcctggtac aaatcttgat tttcgccttg tgataactct aaacaaactt
     4321 gttcccacct ggtcagtgaa tccagggctt catcacaaaa cgaagttatc atttccatat
     4381 caaagtccat aaactatttc ctatgccgct tccatatttg ttgcatttaa aagctcaacc
     4441 gcatttttct gaagaagctc caattccccc aacacgtgct ctgtatgcat gattgttaaa
     4501 atgacttttt caaaagcgcc tgtgctggct tccaaggccc ctaagatatc attcactggc
     4561 gtattgatga acccgatcaa ttgccccatt tccaccagga agtgcttaaa catcgaggtg
     4621 atatcaaccg accgggagcc ttccagcatt cctccggttt ttatcagatc caatcgcacg
     4681 atcaccttgc tcaattcggt cgtgtctttt cttagtctta ataagatctc atagaattga
     4741 ttttgttgtt gagaactgac cataaatagg tccttgaccg ccaggatgat cactctcaat
     4801 tcacttagta actttcgaat ctctgcttca ctggaaattt tccccgcact cattccatca
     4861 atggattctt taagaaagaa cgccaacatt tcggtttgaa cacgagagca ggaaatactg
     4921 aaaagaactt cttctaactt agacaggact gcggcggaaa acttttcgaa attggcatag
     4981 ctctgaatta tttgtttcgt ggttccttga aacgtgtttg ccacaacagc caaggtactt
     5041 ccatccttgc ctaacttatg cgcggaaata gacatattaa tggcgaggtt ctccaacctt
     5101 tcgcaggcac gagctataac cttggattca gatccgcact tttgttttaa atccttgaag
     5161 acctcaaggc tcttgaccgt ttccgcgaag ccatgagccg ccactagcac caactgatgc
     5221 atttcgcaaa atccgcgggt ttgagaagta tcacagcagt gggttttttg tgaacccgac
     5281 gttggtatta gactttgatc ccttaattga agctcagttt tcaaggcgta ggcctggaag
     5341 ctttcataat tttcaaatcc agcttttttt acaagctgaa ccatatgagc taaagccgca
     5401 tcgatccctt tttcgttttc aaccttaagg acctctttgt aaatatcttc cactacttgc
     5461 aaaacaccgg aagttggttt caatcgaata gaaaaatatc cgccttttac agggaacacc
     5521 gttgccaaga cccaataata ctttccagtt gctgatctgt tctttacgta ggcagcgata
     5581 attttacctt gcttaattgt atcccaaagc aaacgaaata ccgccttcgg catatgagga
     5641 tgtcggatga tgttatgagg gctcccgatt aattcctctt tagagaattc tgatactcga
     5701 acaaacacat cgttaccaaa ctgaataacc ccgcgctcgt ctgtacgaga tataaataac
     5761 tcggcaagac caaatggaga ctccaggttt tctaatattt gattactttt catcgatact
     5821 tcccgtcgag aattcctcat gaaacttcaa catctcttcg gtataggcca gggaagtaat
     5881 tagtccagga atataagaga tggagtgaaa agagacgttt aagattaaaa gggaggtctc
     5941 tttggctcct ctcgcattta taatccgcag caaaagaagt ttatcttttg caatcaaagg
     6001 aaattccaaa tactaaaggg gccaacaaac taacttaagt tgattacttc gcttcactgg
     6061 tatcaaaatg tccacacaaa cttaggtata gtaaatgcaa taaaagaaaa tattaaccaa
     6121 ggaggttatt atgacactat ttagattaat accggcagcg tgcttgctgg ctctttcaat
     6181 ggtttccgca ggacaggctc aagttggtag aatcgaccca ggtggcaggg ttgtcatcac
     6241 ctgttcgagc agtgggtatg cttacaacac ctgttacgta gggcagcagg ttgcctatgc
     6301 tagattggtc tctcaattat ctcgatccgc atgtatagaa ggaagcactt ggggctatgc
     6361 aaatgattct atttgggttg ataaaggatg tagagctaac tttgaagtga ttacggctga
     6421 ttatggccgt cctccgggcg gaggaggata tcctcctcca ggtcgtggtg gatacccgcc
     6481 tccaggtcgt ggcggatacc ctcctcccca ccatccgcct atctatgaag aatctattaa
     6541 ttgtgctagc tcaggctaca gattcaatag ctgctacccg gctagtgggt taagaatccg
     6601 atctgctcaa ctttctcgcc aagattcacg cacccaatgc gttgaaggca atacttgggg
     6661 ttatgacaat aatagaattt gggtcgataa aggatgccgt ggagaattca gagttcagac
     6721 ttattagtca gttcagggct gcgggatccc accgcagccc gcttcgtcac tcaaaaaaag
     6781 aatagatggg gtaatggtca gaaccttttc tctcgttaaa tcttagccaa ggcttgattt
     6841 tatcagtgcc actattttta aaaagaatgt gatcaagatg ctgagtgaag ccccaggact
     6901 ggaaagtgta gtccttttca gaagaagcat aagttggttg ccatcctcga tctttaaaaa
     6961 aatcataagt taacttcact tctttttctc cagtaactcc cgatccccag ttcgcgatgg
     7021 aatccgaagt attaaaatct cctaagagga tctgggttcc tttaaaattt attttatcta
     7081 aggccgttat aaggctcaga atctgtttcc tacgatctaa ataagaaatg tctttatagt
     7141 gaacctcgtg atctgtattg atcaccataa tctctcgacc ctcgatttga atccaagcaa
     7201 caagggcttc tctatttttt ttgtccgtat gctctaacgg aatgaccata ctcttgagaa
     7261 ttggatattt actaacaatc gcatggccct cgatatttcc atcatcatct ttagcgactt
     7321 tctgggtaac aacataagga tatttaccta cagggagaat accgtgtagt aatagattgt
     7381 cttgatcaga atctcgaaaa cgcacttctt gaaatgcaaa aatagagata tgattaagat
     7441 ttttcaaatc cctttttagg ccttccaggt cgctcatatt gtgtaaattg tatgtcccaa
     7501 tggaatatgc aaaaacttga taagattgaa aaatcaaaac taagcttgcc aatagtattt
     7561 tcatttcgac tcctttaaaa aattcctgct gcgagtaatt tacactgaat tgcggatttt
     7621 gtatttgaat tccctcatga gggctttatg ccttcctgga gagggttcaa tatagaaata
     7681 attcatcatt gttgggatta tgatcaagtt ttttacataa aaataagaat tacagacatc
     7741 gtaaattatc atttggtttc tctagaatct atggcaaata attctggata ctagcaatag
     7801 acccctaagg agggtccatc atgaaaaaaa tcttagcttt atttcttgta ttcagcttgg
     7861 cgccccttaa atcatggacg gaagaaacca ttcaggaccg cctgcgtaat aaaaaactgg
     7921 ttgctgccca tcagggtgga gtctttgatg gcacacccaa cactctccaa ctatttgagc
     7981 atgcgagacg gaatggagcc gatattattg agatggattt gcatttgtct aaagagggca
     8041 ttccggttgt ttttcacgat gacaccttag actccttgac tgattgccaa ggcaaagtaa
     8101 gtgaccttac agtggctgaa ttaaaaaaat gtgaaattag ctttgtcttt agaggtggaa
     8161 atattccgac ctttgaggag gttcttcagt ggaataacgg aagaattatt ttaaatgcag
     8221 agtttaaagt tcctgaggcg gtagttcccg ccattcgttt ggtggagaaa tataaagctt
     8281 attcttcagt ttactttcaa accaaggggg atccccaatt atatacctcc gcaagatctc
     8341 aaaatccgaa agtggcgtta ctctttgccc cacccaatgt tgagtcctta aactgggcgc
     8401 tttctctcaa tgacgatttc ctcgtggtga ttgaattgca tgagaactta agaaccaagg
     8461 agataatcga tgcgatacac cgggctcata agtgggcttc tgaaaattca tggcattggc
     8521 gttttgatca cgaatttttt ggcgtgtcct gcgccgaagg tttttacgag atcggtttgg
     8581 atattcttgt ttctaatcgt ccgaagcagt gcggggccga tcgcgctgac tttagaatct
     8641 cactcaaggc caaagactat aatccgagat gaatgggtct gagttttcca agtccgtgac
     8701 tgcaggcccg ctgcgacggc tataagctgt ttaccattcg tctcatagga aataactcct
     8761 ccgccgatag gctcgccaat ttttgattgg taaagaacct tccctgtttc ggcattcaag
     8821 gctagaaaat ttccatcaag atcgcctgta aacacaaggc cacccgctgt cggagtgact
     8881 gccgctatta aaggggtggc tgcttgatat tgccagcgaa catttccatc attggcgtcg
     8941 aaagccgtga gccaacctct ccaatttgat ttgggatcca ttgtcccgaa gggttccctc
     9001 ggatctgcgc cggtaaatgg ttcacctgga atcgccttgg cgggaagtcc gctttccgca
     9061 agcttcactg tggtacacca atccgtcgct ggcacaaaaa gagtgttcag ggtgggatgg
     9121 taggcagcgc cattccattc agaacctcct tgggtccccg ggcagaatcg tgtcccagaa
     9181 accgtcattg ggacctcctc attcaaatga ctggtcgtgg gtttcgcgag aatttcactc
     9241 tttatatcca aatccacaaa gtacagatag ccgtccttgc cagcttctgc catcatcaaa
     9301 tgtccgttag aggctcgata aagcacgggc actgtggaga catcccaatc gtgaatatca
     9361 tcctttgtta attgatacca actcttcagg gttcctgtaa aagcatcgag tatgacaaca
     9421 ctgtcagagt aaagattcaa tccaggacgc actgatttca caaaatctgg agcggcattg
     9481 ccgactggaa tcaagatgaa accattcgct tcatcaatag tataagaagt ccaagttgca
     9541 gctcctgttc gcggcacttg aactgaagac aatggccaag tttctgatcc cggtccggtt
     9601 attggagcaa gctcaaagga ccaaagcttc ttgccatcgg actcctgaaa ggccatcagg
     9661 cggcctctca ctccgacatt gtctcctcct gcttgaccga tgtagacgat gccgttccac
     9721 accaccggta cagccggaag actttctcca acatccgggt ttgccacctt ggtttcccac
     9781 aaaagggctc ccgtcttcgc attcaatgca attaaatacc catcgttaga tccccggtaa
     9841 acatgttcgt tcgcaaagaa ggcgccgcgg ttcacgtgaa gcgaatttgt tgccgtcttg
     9901 aattcgcggt gatacttcca aatctgcttg caagtggcgg catcaatggc gtaagtgtca
     9961 ttctctgtcg taatatacat cacactctga cttacgaccg ggccattttg aaaattgagt
    10021 ttttccttta gatcaaagat gcatcgaact tttaaccttc cgatattatt ttgatcgatt
    10081 tccgccaggg gcgagaaacg ctgtgaatcg agtgtcccat taaaacccgg ccaatccccc
    10141 ggaactacat tgacctccac ctgccttgca tagctcttaa atgctatttc tattgataaa
    10201 atgaatgcaa tgaaaaccac aacggcaagg atttttttcc ttgtaggcat gagaaccccc
    10261 tcacaattct tgaatcgatt atttctcatc gtatggaatt taagaaccga ccacaagaaa
    10321 ggaggagatg tttctttgca accaccggtc tgatgaatgg cagatagccg ctcacaactc
    10381 actgcaacga gagccaaaac ggaaatttca aggtgagctt cgccgctctg aaaaaaatca
    10441 gtgcctttgc tcggcaatta aactaggttt tctaaagctt attaattggg ggaaatatgt
    10501 tacgtttact ttatcgtctg ttttattcag tggcttttat tttgggcttt tcatttcact
    10561 cgctagcaag cagtcctttg cttccattcg tcaacggtgg agataaagtt cttgccgatg
    10621 atcctatcgc taaatccaca gtcatgcttg aaactgataa gcaatactgt tcggctacga
    10681 ttatcagtga taatatgtta ctcactgccg cccattgcat cggtgaaaat gagccttggg
    10741 tgttgattca tttctcaggc cttgaaagcg ctctcactcg ctcagccagc cggtctttgc
    10801 gccatgaagg gtatcaagat ctacaagata caactcgcaa tgatatagcc cttgtctttt
    10861 ttgaaggggg cctccccgag ggttttctgc ccatgatgat tttgcccgct gataaagatt
    10921 tagcggtcgg tgacgagtta caaatcgcgg gctatggcgc tggaggtccg ctcggatctc
    10981 tggcaaaggt gagtttaaaa gtcagtgatt ttctagatac acggagattg attaaattcg
    11041 cccagaccac ccatcgaggg atttgccacg gcgattctgg aggtcccgct tttaaggtcc
    11101 tcaataacca attgtattta gctggagttg cctcctacgc caatgagatt gattgcagtg
    11161 actattccgt ttacacccgg gcaacgaact acattgactg gattctccag aagcagaaag
    11221 aataaaatgg agctatctga gagctgactg gatcattgtg cctaattttc agacaggcgt
    11281 ataaaaaaaa tgccattaca tttttgaatt ttcaattcaa gacaaattta ttatagactg
    11341 cgccgatcca aagccctttt tattttcggg gctattaaag ataaggacag ttatgattaa
    11401 aaaaattttt tcacttttac ttgtgatctc acttaacgcc tgtttcgcgc aagcttcttc
    11461 tgatttgcaa attcattttt tatccatccc ctacggaagc gaggtgctga acctgcggat
    11521 tggaatccaa aatcctgacc aggttgcggt tggagatatt ttatatattc atggatttgg
    11581 ggaccgtctt gacaatcatc aacctttatt tcgtgcttgg acaaaagcag gctttcgagt
    11641 catagccttc gatctcccct ctcatggaga aaattctgga acctacaatg atttaaataa
    11701 ctttagcttc gaggagcttg ccgctttagc agtcaaagta gagggcgaaa ctaagcccga
    11761 taccgcacga cctcttattc tttccggatg gtctacggga ggcctggtgg tggttcgaat
    11821 gatccaagaa aaatggatgg cagacctgtc tcggcccgtc agtggtgtga tcttatttgc
    11881 tccaggtatt tccgtcagaa aattcccatg gactttcgga aatcgcttgg gaatggtgac
    11941 ggaaaagacc ctgacccacg atcctcaccc accgcatgta gctcctgtcc gtcctgacac
    12001 ccctttttgg agtcatctgg cttttcaatt ttccccaagg cttgttgctg aatccattct
    12061 ttcacagcac cgcagttatc cgacagacat tccgacttta atattcactg gcggcgatgc
    12121 tgaagatatg tacgctaaag aatgggtggt tcgctcttgg atccaagaac agaatcgggt
    12181 tcgacaatct tcgcaaagct cccccattgt caatgtcgcc tgctccggag caagacacga
    12241 gctggataat gaaaccgagt ctttcggtgg cctagaggtt caaaatagct cagcagcttt
    12301 cgcacaagcg attgcagcag gaaaacttga aatgttttcc gctggaaaag atacttttgg
    12361 gaagatttgc aaatgattta ggagcataac tcagcgttcc catggaaccc tattgccttt
    12421 actattttgt gagaaaaaag atcgatgtag tctaatcgtc ttatcttaaa gatatccaag
    12481 taatcatttt atttaaaaat ttaaattcga aaaaaggaga gcccatggaa atctatgaga
    12541 tcttaaaaaa tgatcatcag gaagtgaagg accttttcga ggatatagat gaattcctaa
    12601 gaaatgggaa ctttgaagag gccgagagtg cttttgaaac ccttcgtgtc gaattgacgg
    12661 ctcattcgaa agcggagcaa gaagtttttt atcagccttt aaaagttata agcgaagatc
    12721 tcgaagatga tgaggtctca ggtgaagagc ttttaggaga ggctgaacag gagcatcacc
    12781 tggtcgctct gcttttaaat gaactttcac gtttagatgt ggaagaggaa gattggaaag
    12841 cgaaactgac agtccttcag gagctggttg atcaccatat tgaaacggaa gaaggggaaa
    12901 tctttgacga agcaagaaag tgtttttccg cagaggaagc cgaaatgatc gcggaaaata
    12961 tgcaggactt aaaagagaaa tacaaaggaa tgatcgaaag tgccttggcc gaagatatcg
    13021 ctcttttcaa tcaacccata agaaatccgg tggatgcctc acaaacgtct ttcagccggt
    13081 agagatccta cttcagctgc tcaccagtac tttcgcttgc agtccggtga gttcatgata
    13141 ctgcctggcc acaaaagagc ccacagtctt actagtaccg ggtctggtga ggccgacaat
    13201 ggatccccca aaaccacccc ccgtaagcct cgcaccgtaa acatccccat gttcccggca
    13261 taatcgcact aaggtgtcaa tttccggaat cgaaacttca taatcttgtg ccatcgataa
    13321 atgagattca taaaacaact ctccgaggcg taccacctcc cccttgagaa gggcttgcac
    13381 ggcggagtgg actcgttgat tttcagtgat cacatgccgg gcgcgacgtt gcaaaacagg
    13441 tggaagctct tctagtttcg gcaggtcggc aagggtcagc tcacgcagtt catgaatttt
    13501 taaacggcca caggcctctt cgcattcgct acgccgttga ttgtaatctc ctccggcatg
    13561 atcatgcatc actcctgaat tgatcactaa taaatccatt ttgtccagac acagaggaat
    13621 gtgctggtag gccagagtct tggtatccaa aaataaggct tctccttctt ttgccaagca
    13681 acaagccatt tgatccataa tacccactcg ggccccgaca aattcattct ctgctctttg
    13741 accaattttt gcgatttcaa tatccgacaa attcagcgag aaagcttcac gcaaggcttt
    13801 taaaaagctg acctccaagg ccgcgctgga agacagtcca cttcccaccg ggaccgttga
    13861 atccacctga agcttaaaac cagatatttt cattccctct tggtcgagga tcctcgtgac
    13921 tccttgaata taatctatcc aggtttttgt cggagcttct tctcctaaag agtaataaat
    13981 ttctcttccg ccgccttcct cgagattgct ggaagaagcg gaaactctgc gatcttcccg
    14041 tctccacaaa gagaccaagg tcttttgtgg aatcgtcgtg ggcagcaccc aaccaccatt
    14101 gtagtccgta tgctcaccga tcaaattgac tcttccatga gcctgcgcag taatgttttt
    14161 catgatttca cccatacttg aggacgagat ctttctgcaa aggaagtcgt cggccagtga
    14221 gggtcgaccg ttaaaatttc gattccgttt tcactcatta aaactgtatc ttctgttttt
    14281 gcccccggca gactgggatt ccacgctaaa gccattcctc gagacaattt cacttgtggc
    14341 ggagattccg gggaaaccac atactcccga gaaagatagc ccgtgggtcc tccctggtga
    14401 tgacgctcga cttcaacagc gcagttaaga tttcgatacg cccccgctat ttctttaaaa
    14461 agttccgtca aagttttgcc aggaaccgag gagtgaaaag ccgcagcctc aaccgcaaga
    14521 gtcatagcga acctcctttc ttcatcagac gtcagcggtc gaaaataaat aaatcgcgtc
    14581 aagttcgcat acaagccaaa gcctcgagcg caaaatacca tcatcgccga ctcacctaat
    14641 ttttcggacc ctgcgatagg atgacggtga agcaaacaac gtcgatgact gccgaccatc
    14701 accaaggcag gatcaagtcc ccgagtccac aaggctctcg ctccttcacc agctaactga
    14761 ttttcactcc agtcaggttc ggccttagtg agggcctctg tcatggcctc tgccgctaat
    14821 cgtccgacct gtcgataacg ttgaatttct tcagggccca aaatcagctt taaatcgcga
    14881 aaggaatcag ggattaactt ttcgcttccg ctaggccgat ccgagtagca ggccccgggg
    14941 ctgtgttgtt gaataaattt ttccaacaac gaagcttgct cccatggaaa ggccataatt
    15001 tcaaattcta gaggcacttc ttcctgggcc aaacgatctt gttcaatttt attggtcaaa
    15061 acccaggctt tggtggcggt gatgaaaatt tccgccacac caagttcact ggtaaaaata
    15121 actgtgctgc gtcctccaca cgttgcccaa ctaaaccaat cgacaccttt aaggcggagg
    15181 gtctgagttt tttccgaaac catttgctcg cgcaatttgt tgatctttat cgcagcttca
    15241 gtcatcacag tgtcacctca acagctcgca gttcagcggc cttttcttca ggaagagtgt
    15301 cgttaatgaa aaatcccgcg ccaagctccg tcccggccag atatttcaag cgatctttag
    15361 tccttaaagg tggaaatatt tcgatatgga catgggattc gggatgcaca tttccatctg
    15421 tgggcgcctg gtaaagcacc atcaaatagg gaaatgttct attccacaaa ttatcgaatt
    15481 tcagcagcac cgtttttaag gtcttggcga agtctttcaa ttcttggtta ttcaaatcat
    15541 agagccaagc ggaccggcgc ttaggagcaa tccaagtctc atagggataa cgtgcgcagg
    15601 gaggaatgaa agaaaccacc ctttcttctt catgaatcaa gcggttttgt tttattactt
    15661 catcttgaac taggtcttcc aataagcccc gttgattctc ttcaaagtaa agactttgca
    15721 tttcgaacat gcgttgcgga atgggcggca ccagaggata acaataaatt tgtccatgag
    15781 gatgatgtaa agtaacgccg acttcaaccc cacggttttc aaaaggcata acatattgaa
    15841 tttcttcgcg gcttccgatc actcttgttc ggtcggccca cacctgcaac aacaactcaa
    15901 tatgatccaa gggtagggcc cctaaacttg tatgagggtc ttgcgtgaac accacaactt
    15961 cgcaatgccc ctttcctggc ttagtgggaa tagaaagctc tggactctct tgcgctcggt
    16021 ccgtcaaaga aggaaatagg ttttcaaaga cggcgatgtc ataatcccca gccggaagct
    16081 ctgtagggaa ttcccgcgat cgagtcaccg ccagaggaga atattcttta ggcggcagaa
    16141 aagtgcggtt ctgccggtga gccgcgtagg cgacccattc accgcgcagt ggatgccaac
    16201 gcaaatgagg agtgcccatg acttctacat gaggaggatt tgtgggaaga atagagggtg
    16261 aaatgggacg acgcgagtaa agagtcagtc cacgaccatc aggctttata aaatctcttt
    16321 tttccattat agccctctgg atgcaaagaa tgccattgcc aagcatgttt tacgatagac
    16381 tgtatttcgg gatactgagg tcgccaattt aaaacctcgc gggctttaag gctgcttgcg
    16441 actaaagccg gtggatcgcc gggccgtcga tcctcttcct tcaccgggag cttcaaacta
    16501 gtggcttcct cacaggctcg aagaacttca cgcacagaaa aacccttttc acttccaaga
    16561 ttaaatacct ctccgtgccc aggctgcgtg gattcaatgg ctaaaacgtg agcacgagcc
    16621 agatccacga catgaacgta atccctcaga caagtgccat cgggggttgg ataatctgtc
    16681 ccgtaaattt ttaccggatt ttgcgtatct aatgccacgg ctaatattcg cggaatcaga
    16741 tgagtttcag gatgatggtc ttcaccgatg gtgccatcgg gatgagctcc agccacgttg
    16801 aaatagcgca aaatagtgta ccccagccca taggccctcg aataatcttc gatcgccatc
    16861 tcggtcatca ttttgcttcg gccgtaagga ttaatagggt gccggtcttg agtttcttcg
    16921 atcggtattt tttttggagt cccataaacc gccgcagtcg aagaaaatat caagcggaga
    16981 acctgggctt tgcgcatggc tcctaaaagg cagagactat gcacaaagtt attctgataa
    17041 tatttctcgg gatcagccac gctctcggcg acttcaatgt ttgcggcaaa atgcattact
    17101 acgtcaatgt gatgggtttt taagatattc agcaaaagct cttcattgcc ggtgcttcca
    17161 ataatcaatt ccgcctttgg atccacggct tcacggcgcc cgttggaaag atcatcgaga
    17221 acctctactt gatgcccttg cgctaacgtt tcgcgaaccg cgtgacttcc tatgtatccg
    17281 gctccgccgg tgattaagat tcgcattgtt gttctcctct cgttcagaca gctgcagggt
    17341 tatattttca aagtggatgg tggctaaatt ttggccactg caaaatattt ttttcaagac
    17401 agatttttgc cagatctaaa gactctccta tcaccacatg catatcgaga tatcgatatg
    17461 ttcccatgcg gccaataaaa gtgatcttgt cctctttttc gatcaagctg atatattttt
    17521 ctaaaaggct tttatcgctg tttaggcgca gtggatagta agggatgtcg ttttcagtgc
    17581 aaaaagcact gtactctcta aaacagacgg tttgcgcatg ttcttcccaa ggtgcaaaat
    17641 gtttgtgttc agcaatgcgc gtatagggaa tctcctcggc gcaataatta atcactgcat
    17701 ttccttggta atccccgtgc tcatcaaatc gctcaaattt cagagttctg tatcccaggc
    17761 gtcccaaatc aaaattaaag taagcgtcca tgggtccact ccaaaataaa tgggcaaatt
    17821 catttttctg ttcacgcggt aggcgtttgc cgagatgaac ttttatgtct tcatgatcca
    17881 gaatattttc aataatttta gtgtagccct cgacgggaat gccctgatat ttttgattgt
    17941 agtaattgtc atcgtaattg aaacgcaccg gaagtcgctt caaaatggag gccggtaact
    18001 cggtgggctc tacgccccat tgttttttag tgtacccata aaaaaaattc ttatacagat
    18061 cttttcctaa aaactttaag gcttgctctt caaagttttt aggctcgtcg acagaaacgt
    18121 cgccttggga aagcataaag tcgtgggcct cccgcgggtt catttttttt ccaaagaact
    18181 ggttcagcgt caacaaattc ataggcaagg aaaaaacccc tcgcttggtc acggctttaa
    18241 cctgatttct aaagtctccg aatttccccc aacgattgag gtaattccag acttcctctc
    18301 ggctggtatt aaaaatatgc ggaccgtatt gatgaatcat tatcccactt tgcggatcac
    18361 gaaaggtatg acagtttccc gccacatggg atctttcgtc ataaactgtc actgtgtagc
    18421 cgccgctctc ggccagctct cgggccagga ccgcgccagc gaagccagct ccagcaatgc
    18481 caattttttg tttcataaat actccttcaa attctgattt ctaattcaga aaaatatcta
    18541 ctcacggcat tttcaaatgt tggcatgact tttatttttt cacttgtgag aacactgtta
    18601 cgaggacgtg gagcgcgaag attcagctcg gcgagatttt tttcaatgat caacgaagtg
    18661 tctaaggaaa tgtctaagca agtgtctaaa caaaagttcc gctggttcgc ttgatctgcg
    18721 acgactctag caaactccgc ccatgagagg gccccttcat tcgccagatg aagcaagccg
    18781 tactctccgt ccaagagtag atcgaggcaa gtatgaacca gatccgggat gtaagtcgga
    18841 gaaactttta tgtcgcgaca aacaaaaacc ttcttcttaa gtgacaggtc tttcaaaata
    18901 ttcgtggcaa aattaaattg atcccaagga ccgaagaaag aactcgttcg aactaccagg
    18961 gcttccgagt aaagacttaa tactttaact tcactctcgg ctttggaacg accatagaca
    19021 ttgagcggcg atggtttgtg actttcaaca taaggtattt ccaaagtacc atc
//
LOCUS       DATBTE010000260         4455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2357901, whole genome shotgun sequence.
ACCESSION   DATBTE010000260 DATBTE010000000
VERSION     DATBTE010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2357901"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VIG33_09705"
     CDS             <1..781
                     /locus_tag="VIG33_09705"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8271149.1"
                     /translation="APWVWIVYVVTVMLFYIPLIGLFIGAFIKKENEEWLWTLQWFVE
                     VFQDENLTQGLVNSFLVGGSASLISTFLGTTAAIGLQHTDGWNGRWLQGMSFLSLFLP
                     EIVFALSLLSWFFILQFELGYLTVIIAHVTLTISYVILTVGARLSLLDRSLEDAASDL
                     GANPFKTLWYITLPLLKPAIFSGLLLSFLISFDDFLITFFVNGIGKDTLPVKLYTSMK
                     MGLSPKLNALSTLMLFVTLTVLLLIFKNPAVRDLLQNKKKS"
     gene            complement(918..1520)
                     /locus_tag="VIG33_09710"
     CDS             complement(918..1520)
                     /locus_tag="VIG33_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271150.1"
                     /translation="MVAENVFESCRFEIKNPGKSDQKFEGIPLSTRDRAVEACYDGVD
                     LIYLSSSKYPLAAKNSLEKRRLIAHEVFLKMGLEGDDWKLSTELKGLPLNKVAVCHIC
                     GDEEAIAITKSFCSENQEIEVDLTFGTEGTLHFDRYSIEIWAIPNEQPRIYLFDSLRH
                     VGASGRFDSQTYDSVVQLLGDPRKPTPANKVSATAICKLK"
     gene            complement(1559..1873)
                     /locus_tag="VIG33_09715"
     CDS             complement(1559..1873)
                     /locus_tag="VIG33_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271151.1"
                     /translation="MKSLKLVAIGICLMASASFAQAFSNGNITCTLSNGTVVNFDIID
                     CTASISKWVAGGIFPHGGWGISFTMNGSNACKGRRNLHLDYSNGVLYNGSYSSAASCE
                     AW"
     gene            complement(2353..3456)
                     /locus_tag="VIG33_09720"
     CDS             complement(2353..3456)
                     /locus_tag="VIG33_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8271152.1"
                     /translation="MKIEKEVVHFEANFTGLSDLTDLQKLYLQVLQGGKSLEAVVHHF
                     LQQGWLVNFVELADLVEKLVTNKSLRNLNFYVYFDKMKPTSERSLWNKIVNLDFPESS
                     MGSMRNYKELPFFRTLEPQLTDFLLSKATMHHVPPKSLICRQGETTRDLFVILKGTAG
                     IYKPHQQSGKYLVATLSDNAVFGEGAFLLGHPRSADVISLSDCRILRIPCLPEIFDRY
                     LKKDKAQGLQYRFWVQHALLNSPLLKEVPSDCFDALSYSGKFVQCPEGQVLFHEGEKG
                     HSVYIVVQGSLVVNKNGQNINVLNQGGILGEIALLANQGLRSASVFAQRDSLLIEINQ
                     AEFYKLLAQNIFLGKYLQELAYSRLLKDEKRAG"
     gene            complement(3459..>4455)
                     /locus_tag="VIG33_09725"
     CDS             complement(3459..>4455)
                     /locus_tag="VIG33_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271153.1"
                     /translation="KVTEPMSVACVRSLFELAEELEKTLPNSAGSEFKLKAGLFSGQI
                     QFTKLESGFSLSGVPFAESAKMLAQSHKIKENSALIVSESCREFVSRICHLSSLSSDL
                     CFVDAFTPQGFIFENAKVEYCTFFRSDLNLMALMNYMEKLVDEQDGELCFRIFGDLKF
                     IKAQVICSDLEKSFTHFLEFAYRKSHNKPVDPKILSAAVSLTHNLIPAGCGSEKLTEI
                     LKLYLQFSEPRVQANAVAVLGEVPSNVHFLKKFIHSDHNRLSADALLVTGKQVVDKEL
                     ITRVQRLLRSSDKLHQASGRYVALKLVEHYKQNDKLYYNTNPHLKKLEELLGKAA"
BASE COUNT         1293 a         1000 c          873 g         1289 t
ORIGIN      
        1 agcgccctgg gtgtggattg tttatgtggt gaccgtgatg ctattttata ttcccctgat
       61 agggcttttc attggcgcct ttattaaaaa agagaatgaa gagtggctat ggactctgca
      121 atggttcgtt gaggtttttc aggacgaaaa tttaactcaa ggcctcgtga attcctttct
      181 agttggcggc agcgcttctt tgatctcaac cttcctcggt acaacggccg cgatcggttt
      241 acaacatacc gatgggtgga atgggcgatg gctccaagga atgtcttttt tatcgctctt
      301 cttaccagaa attgttttcg ccctttcttt gctgtcatgg ttttttattt tgcaattcga
      361 actgggttat ttaaccgtca ttatcgccca tgtgacgctg acgatttctt atgtgatact
      421 gaccgtggga gcccgcttaa gtttattgga tcgcagccta gaggacgccg cttcagatct
      481 cggtgcgaat ccctttaaaa ctctttggta tattaccttg cctcttttaa aaccggcgat
      541 ttttagcggt ttactgctga gtttcttaat ttcatttgat gattttctca tcaccttttt
      601 tgtaaatggc atcggtaaag acactttgcc agtcaaactt tacacctcta tgaaaatggg
      661 cttaagccct aaactcaacg ccctttcaac cctgatgttg tttgtgaccc tgacggtgct
      721 ccttttaatt ttcaaaaatc ccgcagttcg ggatctgttg cagaataaga aaaagagtta
      781 agaatcaaaa aagtgatcag acatggacaa gaagaaaaga gaaggcacag tttttaactc
      841 tgcgcctttc aaggagtgtt ttggacaaaa gcaagggatt taaacagtat tcttttagta
      901 gtcaaatata agagtgatta ttttaactta caaatcgccg ttgcactcac tttatttgcg
      961 ggtgtgggtt ttcttggatc ccccagaagc tgtactacac tgtcataggt ttggctatca
     1021 aaacgacccg aagccccaac atgtctgagg gaatcaaata agtaaattcg aggctgctcg
     1081 tttggaatcg cccagatctc aatggaatat cgatcaaaat gaagtgtccc ttcggttcca
     1141 aaagtcaaat caacctcaat ctcttgattc tcactgcaga aagactttgt gatagctatg
     1201 gcctcttcgt ccccacagat atggcatacg gccaccttat ttaagggcaa acccttcaat
     1261 tcagttgaaa gtttccaatc atcgccctca agccccatct taaggaaaac ctcatgtgcg
     1321 ataagtcgtc ttttttctaa cgagttcttc gctgctaatg gatacttaga actactaaga
     1381 tatattaaat ccactccatc ataacaagct tccacggcgc gatcgcgagt agacaaaggg
     1441 attccctcaa atttctgatc tgatttgcca ggatttttaa tttcaaatct gcaagactca
     1501 aacacatttt cagccaccat aatactgcac ttatggtttt ttttgataag caaggagctt
     1561 accaagcttc gcaacttgcc gcagaagaat aggaaccgtt ataaagaaca ccgttgctgt
     1621 aatcgaggtg cagatttcgt cggcccttac aggcattgct gccgttcatg gtaaaactaa
     1681 ttccccatcc gccatgtgga aaaattccgc ctgcaaccca tttcgaaata cttgcagtac
     1741 aatcaataat atcaaaattc acgacggtgc cgttgctcaa cgtgcaggtg atatttccgt
     1801 tactaaaggc ctgagcgaaa gaagcggaag ccatcagaca aattccaatt gctactaatt
     1861 ttaacgattt cattcttatt ccctttgtta agttattaag ttttattttt caaatttatt
     1921 aaaatctgta caacatcaat atactagaaa gatttcattc tcggagattt agatccgaga
     1981 ataaaactgc ctttagttca tgcaggtatt tcgagcagag gtaagcgtta cccctcctgc
     2041 tttataaact ctttgtacac aatcatttgc tgatacaatt tgcgatccta caatcaaagt
     2101 agaaatcaaa aaaaactgcc ctctttttaa gtattccata aatcttcttt attgataccg
     2161 ttgcaaagtc tgtgggatta atttcccata ttttcagacc gcaatctttt caagtcattt
     2221 tgacctgcta gaatggtgcc gtttcaaaag gcgatttatt ttcaattgaa ggcaaaagag
     2281 tgtttaaaca tcttgtttta agacgttgag tgtcactgtc aagaagttct tcactctatt
     2341 taaaactcga acttaaccag cccgtttttc atccttaagc agacggctgt aagcgagctc
     2401 ttgaaggtac ttccctaaga aaatattttg tgcgagaagc ttataaaact cggcctggtt
     2461 gatctcaatc agtaaactat ccctttgggc gaataccgag gctgaacgca atccttgatt
     2521 tgccagcaat gcgatttcac cgaggatgcc accttgattg aggacattga tattctgccc
     2581 atttttatta accaccaaag aaccttgaac aacgatataa acactgtgcc ccttctctcc
     2641 ctcatgaaag agaacttgcc cttccggaca ctgaacaaac ttgccagagt agcttaaggc
     2701 atcaaagcaa tccgaaggaa cttctttcaa cagaggcgaa ttcaaaagag catgttgaac
     2761 ccaaaaacga tattgcaggc cctgggcttt atcttttttg agataacggt caaagatttc
     2821 aggcaaacaa gggatccgca aaatgcggca gtcactcagt gaaatcacat ccgcagatct
     2881 tgggtgacct aataaaaaag ccccttcacc gaagaccgca ttatcagaaa gtgtggccac
     2941 caagtacttc ccactttgtt gatggggctt atagatgccc gcagtccctt taagaatcac
     3001 aaaaagatcc cgagtcgttt caccttgacg acaaattagc gatttgggtg gcacatgatg
     3061 catcgtcgcc tttgagagta aaaaatccgt gagttgcggc tcaagagttc taaaaaatgg
     3121 caattcttta taattgcgca tggaacccat ggaactctct ggaaagtcta aattgacaat
     3181 cttattccaa agcgatctct cggaggtggg cttcatttta tcaaaataaa cataaaaatt
     3241 taaattgcga agggatttat tagtgaccaa cttttcaacc aggtctgcca actccacaaa
     3301 attaaccaac catccttgct gtaggaaatg atgaaccacg gcttcaagcg acttaccgcc
     3361 ttgcaggact tgcaaataca gcttctgcag atctgtcaga tctgacagac ctgtaaagtt
     3421 cgcttcaaaa tgaacaactt ctttttcgat cttcatcgct aggcagcctt tcctagaagt
     3481 tcttcaagtt ttttcaagtg aggattcgta ttatagtaaa gtttgtcatt ttgtttgtaa
     3541 tgctccacca gctttaaagc cacatagcgt ccgctcgcct ggtgcagttt atcagaagag
     3601 cgcaggagac gttgcacgcg agtaataagc tccttgtcca ccacttgctt gcctgtgacc
     3661 aacaacgcat ccgctgataa acgattgtga tcagaatgaa tgaacttttt taagaaatga
     3721 acattcgagg gcacctcacc caaaacggct actgcgttgg cttgaacacg aggttcggaa
     3781 aactgcagat ataatttcaa aatttccgtt agtttctcag aaccacagcc cgcaggaatt
     3841 aaattatgag tcagggaaac cgccgccgaa agaattttcg gatccacggg tttattgtgg
     3901 gatttacgat atgcgaactc caagaagtgg gtgaaagact tctccaggtc tgaacaaata
     3961 acctgggcct ttatgaactt caggtcaccg aaaatacgga agcaaagttc tccatcctgc
     4021 tcatccacaa gcttttccat gtaattcatc agagccatca aattcaaatc gctgcgaaag
     4081 aaagtgcagt attcgacttt ggcattttca aatataaatc cctgcggagt aaaggcatcg
     4141 acaaaacaaa gatcggagga taaagaagaa agatggcaaa tgcgtgagac aaattctcgg
     4201 caagactctg aaacaatcaa cgctgagttc tcctttatct tatgactttg cgctaacatt
     4261 tttgcagact ccgcaaaagg aacaccgcta aggctgaagc cactttccag tttagtaaat
     4321 tgaatctggc cgctgaacag acccgctttc aacttaaatt ccgatcctgc ggagttcggc
     4381 agcgtctttt ccaattcttc ggcaagctca aaaagtgaac gcacgcaagc aacagacatc
     4441 ggctccgtca ctttc
//
LOCUS       DATBTE010000261         7840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_34655, whole genome shotgun sequence.
ACCESSION   DATBTE010000261 DATBTE010000000
VERSION     DATBTE010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34655"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="VIG33_09730"
     CDS             complement(<1..1453)
                     /locus_tag="VIG33_09730"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEY8271154.1"
                     /translation="MKSNINVNDLILISPMIALFLTSLIPITLKVLRGNREQNALSTL
                     IQGLIGLVIAAVLLGIFSGPAGATAFNHSLLFDGITQWMGIIALLAAAGSLVLMYENP
                     STKGNQFSEIVFLTLSSALGMLILVSAADLLMIFIGLELMSLSLYLMIAMSHEEKLAK
                     EAALKYFVLGGFASAIFLYGVSFIFGSAGSTNILDFLETAKSLVVTSRLFLFGITFVI
                     IGFCFKVSIAPFHAWTPDVYQGAPTPLSGFMATAVKTVSFAAFLRIIATRSLEGSQNL
                     FDVLQWLAVITMIVGNVGAIMQNNLKRIIAYSSVAHSGYILVGLITAGVSEVGSFGAS
                     GVIFYMISYVLMTLGCFAIISMMEKSENSIINVDDLAGLAHRRPLYALCFTIFLLSLA
                     GIPPTVGFFGKFYLFNAAVGEGLLWLTIWGVINSVISVYYYLRPIVVMYMKEGDTEVA
                     GHSLNATTVTVLVSAVCIILLGLSSGPIFTAIEK"
     gene            complement(1453..3072)
                     /locus_tag="VIG33_09735"
     CDS             complement(1453..3072)
                     /locus_tag="VIG33_09735"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY8271155.1"
                     /translation="MLLSAIVFMPLLFALIVTFWPRVSSVRHLALGFSIIEFFLSLSI
                     LQKFDPSSAALQLVEKHMWIERFGISYFFGIDGLSLWLVIMTTFLMPIVILASWTAID
                     KRVNGFHACLFILQTAMLGTFLAMDAIFYYVFWELSLVPMYFMVGIWGGARRIYATVK
                     FFIFTMAGSVLMLVAMIYMMYLTQEATGQMSSSLLDFYKLKIPFIGSQFLSLQTLLFF
                     AFALAFAIKVPVFPLHTWLPDAHVEAPTPGSVILAAVMLKMGTYSFLRWVIPLFPEAS
                     EYWAWLFMLIGVIGIIYGALVAMVQPDIKKLVAYSSVSHMGYVLIGLFTFNTFGLQGS
                     LYQMLNHGISTGALFILIGMIYERTHSREISKYGGLASVLPVFTIFFFIITLSSIAVP
                     MTNGFVGEFLILLGSFKAMPGFTYVAVLGVVLGAVYMLWMFKRVFFGIQGELVKDHHH
                     PLHDLNMREIAVMVPLVVMVFWMGLFPNQFLDWSKASVTHLVENHSGYSLAIYGVPET
                     VNPPAPVTTTAPNEETPAVNEQSTAVEEGAQ"
     gene            complement(3077..5017)
                     /gene="nuoL"
                     /locus_tag="VIG33_09740"
     CDS             complement(3077..5017)
                     /gene="nuoL"
                     /locus_tag="VIG33_09740"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY8271156.1"
                     /translation="MSHSILMSILILSPFVGFLINGFRYKTHSYKVAGSIATTAVVIS
                     FICSVLLVAELIGLPPENRRILVSFFEWINVGNLKIDAAFVVDQISAIMILIITGVGS
                     LIHLFSISYMHHDKGVAKYFAYLNLFIFNMLLLVLGSNLLVMFVGWEGVGLCSYLLIG
                     FWFTDREKAAAGMKAFVTNRVGDAAFLLGMFIFFLTFGTLNFSELNALAPSASDATWL
                     GAVSLGTLFLFIGATGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGVYMIVRLN
                     PMFAMAPNTMMIIAVIGAATAVTAATIGMTQWDIKKVLAYSTVSQLGYMFLACGVGAF
                     GAAMFHLMTHAFFKALMFLGSGSVIHAMHEEQDIRKMGGLKKYLPITHFTFTLGWLAI
                     IGIPPFAGFFSKDEILWQAFHSSYGHMALWAAGAMGATLTAFYMTRLMALTFWGQSRV
                     PKNTHPHESDAMMTIPLIILGILSVIGGWIGIPHVIGEVFGGIPNIWEHWLHPLIKEL
                     PVAAEGGNADASLEWMFMAISVGLAVVSAIVAFQMYVRKPGSAEKVAEGLGPVYRAVY
                     NKYFVDEAYFAVIINPLVKFSRTLWYYIDVNFIDKCTYWVGDIVRGGGSLVRSLQTGN
                     MQQYAMYIGIGLVFVLSILLMR"
     gene            complement(5024..5350)
                     /gene="nuoK"
                     /locus_tag="VIG33_09745"
     CDS             complement(5024..5350)
                     /gene="nuoK"
                     /locus_tag="VIG33_09745"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEY8271157.1"
                     /translation="MPSIDIVTHIGLNHYLVLSALLFVTGMAGVLLRRNVIVLLMSIE
                     LMLNAVNISFVAFSKYLDKLDGQVLVFFVMTIAAAEAAVGLAIAVSIFKRYKEVNIRF
                     FEHLKG"
     gene            complement(5337..5867)
                     /locus_tag="VIG33_09750"
     CDS             complement(5337..5867)
                     /locus_tag="VIG33_09750"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEY8271158.1"
                     /translation="MTADSFLFWMLAAVTLCSGLAVVVISNPIYSALCLAMSMVGVSA
                     LFATLGAYFIAGVQMIVYAGAVLVLFVMVLMLFDLKTELVTFSRGKISGLLKIATVGV
                     LAGFLAGAFSMTIEFAEKTGAATTVPTPDTSKALGTLLFTKYILGFETLGILLLVIAI
                     GVVGLARSKGGTHAKH"
     gene            complement(5876..7003)
                     /locus_tag="VIG33_09755"
     CDS             complement(5876..7003)
                     /locus_tag="VIG33_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEY8271159.1"
                     /translation="MGSDAFEITINCIKLILVFLLMVQAVPVLVWLERRGSGFIQNRF
                     GPNRVGPLGLMQLLADAVKFLTKEEFSPKAATKLLFYAAPVFALIPGTLGFAAIPMGS
                     PLHWGNYVFPIQGYDVGVGIVFILGVSSLGAYTILMAGWGSGNKYALMGALRASAQTI
                     SYELALGLSIVGIIMMYGSFNLSEMIHAQQGTMVFHAFGNTISTSWLPNWGIFYQPLA
                     AIIFFSAAFAESNRLPFDLAEGEAELVAGFHTEYGGFKMLLFFIGEYGHMMVASGLMV
                     TFFFGGYNIPWTTTEQVYQWALGLMDSSDGASALTSLVHFLSYMIKLGFFLWIFIWVR
                     WTLPRFRYDQLMDLGWKTMLPWALANTIVSAIVIFAVSYSW"
     gene            complement(7007..7375)
                     /locus_tag="VIG33_09760"
     CDS             complement(7007..7375)
                     /locus_tag="VIG33_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY8271160.1"
                     /translation="MPLGGVIFITVFVSIFGCLCLYLGRTFGGKKPQDNVKLSAYECG
                     IPSQEKKETRVSVKFYLTAILFILFDIEIIFMYPWATSFKDLTAAGLGAYALIAMMIF
                     IAIFIFGLFWEIKSKALEWD"
     gene            7491..>7840
                     /locus_tag="VIG33_09765"
     CDS             7491..>7840
                     /locus_tag="VIG33_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271161.1"
                     /translation="MKTELLHTRLETLLERAFETKKSKIQVLGSSSELPLAFFLSQTY
                     SNKINDLPHLVIVGSHESAVELKRLLRFFDPLKYCYIFNHFDVSPYSGLYPNSRTIGE
                     RLQFLYHAQNSKPG"
BASE COUNT         2634 a         1852 c         1540 g         1814 t
ORIGIN      
        1 ctttttctat cgccgtgaaa atcggtccag aggaaagacc caacaaaata atacaaaccg
       61 cagaaacaag aacagtcacc gtcgtcgcat tcagagaatg acccgctact tccgtgtctc
      121 cttctttcat gtacataact acgatagggc gaagatagta gtaaacacta atgactgagt
      181 tgatcacacc ccatattgta agccatagaa gaccttcgcc gacggcagca ttaaacagat
      241 aaaacttacc gaagaaaccc actgttggag gaatccctgc gagggataac aagaaaatcg
      301 taaagcataa agcataaaga ggtcttctat gtgcaaggcc cgctagatca tccacgttta
      361 tgatcgaatt ttcagatttt tccatcatgc taatgatcgc gaagcaaccc agggtcatga
      421 gcacatagct gatcatataa aaaatcacgc ctgatgctcc gaaagatccc acttcactca
      481 cacccgcagt gatgaggcca accagaatat atcctgaatg agcaacactg gaataagcaa
      541 ttatacgctt taaattattt tgcatgatgg cacccacgtt accaacgatc atcgtgatca
      601 cagcaagcca ttggaggacg tcaaaaagat tctgagaacc ttccaagcta cgtgttgcaa
      661 taattctaag gaatgcagca aaagaaacgg ttttcacggc agtggccatg aagcctgata
      721 gcggagtcgg agccccttga taaacatcag gtgtccaagc atgaaaagga gcaatggaaa
      781 ccttaaagca gaaaccaatg atcacaaaag taatgccgaa taagaacaaa cgacttgtta
      841 ctactaggct ctttgccgtt tccagaaagt ccaagatatt tgtgctgcca gcagatccga
      901 agatgaagga cacgccgtac aagaaaatcg cagaggcgaa gccacccaaa acaaagtatt
      961 taagtgccgc ttctttggcg agtttttctt catggctcat ggctatcatg aggtaaagag
     1021 ataaagacat cagctcaagg ccgataaaga tcattaaaag atccgcggcc gaaaccagga
     1081 ttaacatccc taaagctgaa cttagagtta gaaatacaat ttctgaaaat tgatttccct
     1141 tagtggatgg attttcatac atcaaaacca aagatcccgc cgctgccagc agggcgataa
     1201 ttcccatcca ttgtgtaatt ccatcaaata aaaggctgtg gttaaatgcc gttgctcctg
     1261 ctgggcctga aaaaatacct aaaaggaccg ctgcaatcac cagtccaata agaccttgaa
     1321 tcaaagttga taaagcgttt tgctcgcggt tgcctcttaa aaccttcaag gtgatgggga
     1381 tcaagcttgt caggaacaat gcaatcattg gagaaataag tatcaaatca tttacattaa
     1441 tattcgattt cattattgag ctccctcttc aacagccgtt gattgttcat tcactgctgg
     1501 agtttcttca ttcggtgctg tagtggttac tggagctggt ggattcaccg tttctgggac
     1561 gccataaatt gctaagctat acccagaatg attttccact agatgagtta cgctggcctt
     1621 agaccaatcg agaaattgat ttgggaaaag ccccatccag aaaaccataa caaccagtgg
     1681 aaccatcact gcaatttccc gcatgttcaa atcgtgtagc ggatggtggt gatctttaac
     1741 aagttcgcct tgaatgccaa agaaaacgcg cttaaacatc cataacatat aaacggcgcc
     1801 caatactaca ccgagaaccg ccacataagt gaaccctggc attgctttga agcttcctag
     1861 gaggatcaaa aactctccca cgaaaccatt tgtcataggt accgcgatcg aagaaagcgt
     1921 tataataaag aagaagattg tgaagaccgg caatacggat gccagaccgc catatttaga
     1981 aatttcacga gaatgggttc tctcataaat cattccaatc aaaatgaaaa gggccccggt
     2041 ggaaataccg tgattcagca tttgatataa actgccctgt aaaccgaatg tattgaaggt
     2101 gaaaagacca atcaagacgt accccatgtg agataccgac gagtatgcga ccagcttctt
     2161 aatgtccggt tgtaccattg ccaccagtgc cccataaata atgccaatca ccccaatcag
     2221 cataaagagc caagcccaat attcagaggc ttcagggaat agtgggatca cccaacgcaa
     2281 gaaagaatag gtgcccattt tgagcatgac ggcagccaga atcactgaac ctggagtagg
     2341 cgcctcaacg tgggcatccg gtaaccaagt atggagggga aatactggaa ctttgatagc
     2401 aaaggctaat gcaaaagcga aaaataataa ggtttgaagg ctcaaaaatt gacttccaat
     2461 aaatggaatt tttaatttat agaaatccaa aagactcgaa ctcatttggc ccgtcgcttc
     2521 ttgagtcaga tacatcatgt agatcatcgc tacgagcatc aatacagaac ccgccatcgt
     2581 gaatataaaa aatttcacgg tcgcatagat acgtcgcgct ccaccccaaa tacctaccat
     2641 aaagtacatt ggcactaatg aaagttccca gaatacatag tagaaaatag catccatcgc
     2701 gagaaaggtt ccaagcatgg ctgtttgcaa aataaacaaa catgcatgaa agccattgac
     2761 gcgtttatca atggctgtcc agcttgcaag gatcacaatt ggcatcaaga aggtcgtcat
     2821 aataacaagc cataaagaaa gaccatcgat tccaaagaag taagagatcc caaatctttc
     2881 aatccacata tgcttttcca caagttgaag agcggcagag ctcgggtcaa acttttgcag
     2941 aatactgagg cttaagaaaa actcgatgat ggaaaatccg agagccaaat ggcggacaga
     3001 gctcactcgt ggccaaaaag tcacgatcag agcaaacaag agcggcataa aaacaatcgc
     3061 gctgagtaac atgaaattac ctcattaaaa ggatggaaag aacaaacacc aagccaatgc
     3121 caatatacat cgcatattgc tgcatatttc cggtttgcaa tgagcgaacc aatgaaccgc
     3181 cgccccttac aatatctcct acccagtagg tgcatttatc aataaagttt acatcgatat
     3241 aataccaaag cgtgcggcta aactttacaa gtggattaat gattactgca aagtaggctt
     3301 catcaacgaa atatttattg taaacagctc tgtaaactgg tcccaagcct tcagcaactt
     3361 tttcagcgct gcctggttta cggacataca tttggaaagc aacgattgca gaaacgactg
     3421 ccaatcctac agagatagcc ataaacatcc actcaaggct tgcatctgca tttccaccct
     3481 ccgctgctac cggtaattct ttaatcaaag gatgcagcca gtgctcccag atatttggaa
     3541 tacccccgaa gacttcacca atgacatgag gaattccaat ccaaccacca atcacagaaa
     3601 gaatacccaa aatgattaaa ggaattgtca tcatcgcatc tgactcatga ggatgagtat
     3661 tttttggaac gcggctctga ccccagaaag tcagggccat caagcgagtc atataaaacg
     3721 cagtcagagt tgcccccatc gcaccagccg cccaaagggc catgtgcccg tatgaggagt
     3781 ggaacgcttg ccaaagaatt tcatcctttg aaaagaatcc tgcgaaaggc gggattccta
     3841 taatcgcaag ccaccccaat gtaaaagtaa aatgggtgat gggaagatat ttcttaagac
     3901 cacccatttt gcgaatatct tgttcttcat gcattgcatg gataaccgag ccagatccca
     3961 agaacataag ggctttgaaa aaggcgtgag tcataagatg gaacatcgcc gcgccaaagg
     4021 ccccaactcc gcaagcaagg aacatataac ccaactgcga gaccgttgaa tacgccagaa
     4081 ccttcttgat atcccactgg gtcataccga tggttgccgc tgtcaccgca gttgccgcac
     4141 cgataaccgc aatgatcatc attgtatttg gagccattgc aaacatggga tttaaacgca
     4201 caatcatata cacgccagca gtcaccatcg tcgccgcgtg gattaaggcc gaaactggag
     4261 ttggacccgc catcgcatcc ggcaaccaaa cgtacaaggg aatctgtgcc gatttacccg
     4321 ttgctccgat aaatagaaat aacgtcccca ggcttaccgc gcccaaccaa gtggcatccg
     4381 aagctgaagg tgctaaagcg ttgagttctg aaaaattcaa agttccaaaa gtaaggaaaa
     4441 agataaacat tcccaacaag aaagccgcgt ctccgactcg attggtaacg aaggctttca
     4501 taccagcggc cgctttttct ctatctgtga accaaaaacc aatcaagagg taagaacaaa
     4561 gccctacacc ctcccatcct acaaacatca caagtaaatt actcccaagc actagaagta
     4621 acatattgaa aatgaataaa ttaaggtaag caaaatattt tgctacccct ttatcgtgat
     4681 gcatgtaact aattgaaaat aaatgaatta aagagcctac gcctgtaata atgagaatca
     4741 tgatcgcgct aatctgatcc accacaaagg ccgcatcaat tttcaggttc cctacgttga
     4801 tccattcaaa aaacgaaacc aggatccgtc ggttctcagg cggcaggcct ataagttctg
     4861 caaccaatag aactgaacaa ataaatgaaa taaccactgc tgttgtcgca atagaacctg
     4921 caactttata gctgtgtgtt ttatatcgga agccattgat taaaaagcca acgaaagggc
     4981 tcagaatcaa aatcgacatc aagatgctat ggctcattgg cgattatccc ttcaggtgtt
     5041 cgaagaagcg aatattcact tctttataac gtttgaaaat tgaaactgcg atagccaaac
     5101 caacggcggc ttcggcggcg gcgattgtca tcacgaaaaa caccagaacc tgaccatcga
     5161 gcttatctag atacttgcta aatgcgacaa agctaatatt cactgcattc agcatcagct
     5221 cgatcgacat taatagaaca ataacatttc ttcttaaaag gacccccgcc attccggtta
     5281 caaaaagaag tgccgaaaga acaagataat gatttagacc aatatgagta acaatatcaa
     5341 tgcttggcat gtgttcctcc tttgctgcgt gcaaggccta caactccgat tgcgatgact
     5401 aaaagaagaa ttccgagagt ctcaaagccg agaatatatt tggtaaataa gagggtgccc
     5461 agcgccttac tagtatctgg agtggggact gttgtcgctg ctccggtttt ttccgcaaac
     5521 tctatggtca tgctaaaggc acccgccaag aagccagcca gaactcccac ggtggcaatc
     5581 tttaaaagac cactaatttt cccgcgagaa aaggtgacaa gctctgtttt tagatcaaaa
     5641 agcatcagca ccataacaaa taaaaccagt acagctccag cataaacaat catttgtacg
     5701 ccagcaataa aataggcgcc caaggtggca aataaggcgg aaactcccac catcgacatc
     5761 gccaaacaaa gggctgaata aatgggattt gaaatcacca caacagcaag accgctacat
     5821 aaagtcactg ctgctaacat ccaaaataaa aaactatctg ctgtcacaaa aactcctacc
     5881 aagagtaaga aactgcgaag attacaattg ccgatacgat ggtattagcc aaagcccacg
     5941 gaagcattgt tttccaccca agatccatta actgatcata tctaaagcgc ggcaaagtcc
     6001 aacgaaccca tataaagatc caaaggaaga aacccaactt aatcatataa gataaaaaat
     6061 gaactaaaga ggtaagagcg ctagctccat ctgaggagtc cataaggccc aaggcccatt
     6121 gataaacttg ttcggttgtt gtccaaggaa tattatagcc gccaaagaaa aaggtgacca
     6181 tcaaaccaga tgcgaccatc atatgaccgt actcaccaat aaagaagagc agcatcttaa
     6241 atccaccata ctcagtatga aaacccgcta ccagctccgc ttcaccttcc gctaagtcaa
     6301 agggcagccg attagattcc gcaaacgctg cggaaaagaa aatgatcgcc gcaagcggtt
     6361 gataaaaaat tccccaattt ggcagccagc ttgtagaaat cgtatttcca aatgcatgaa
     6421 ataccatcgt cccttgttga gcatggatca tttcacttaa gttgaaagag ccgtacatca
     6481 tgataatgcc aactatggaa agacccagtg ctaactcgta gctaatagtt tgagcgcttg
     6541 cccgcaaagc acccattaaa gcatacttat ttcccgatcc ccagcctgcc atcaaaatgg
     6601 tgtaagctcc caaagaagaa acaccgagaa tgaaaacaat gccgacaccc acgtcgtagc
     6661 cctggatagg aaaaacatag tttccccaat gaagtggtga acccatcgga atggctgcaa
     6721 aacctaaagt accaggaatt aatgcaaaca ccggagctgc ataaaataat aatttcgttg
     6781 cggccttcgg gctaaactct tctttggtca aaaacttcac cgcatctgcc aagagctgca
     6841 tgagtcccaa aggacctacg cggtttggac caaagcgatt ttgaataaac cctgatccac
     6901 ggcgctccag ccaaacaagt actggaactg cctgaaccat caataaaaaa acaagaataa
     6961 gcttgataca gttaattgtt atttcaaatg cgtctgaacc catgatctag tcccactcca
     7021 aagctttaga tttgatttcc cagaaaagtc caaaaataaa aattgcaatg aagatcatca
     7081 tggcaatcag cgcataagca ccaaggcctg cagctgttaa gtccttaaaa cttgtggccc
     7141 atggatacat aaagataatt tcgatatcaa aaagaatgaa aagaatcgca gttaagtaaa
     7201 acttaacgga aactctggtc tcttttttct cttggctagg aattccgcac tcatatgccg
     7261 aaagctttac gttgtcttga ggttttttgc caccgaaagt acgcccaaga tacaaacaca
     7321 aacatccaaa gatcgacacg aagactgtaa tgaatatgac tccgcctaaa ggcacagacc
     7381 ctccctggtt gacatataat tgagaattgt atcgttatac tcatgccttg cgtgaaatcc
     7441 aagaaatatg aatggaaaga tgacctaact agtggcaaca tcacacaaaa atgaaaacag
     7501 agttacttca caccagactt gagactcttt tagagagagc ctttgagacc aaaaaatcca
     7561 aaattcaagt tttgggaagc tcctccgagc tgcctcttgc ttttttcttg tctcaaactt
     7621 attcaaataa aatcaatgac ttaccgcatc tagtaatagt cggcagtcat gagtctgcag
     7681 tagaattaaa gcgtttgctg cgcttttttg accctcttaa gtattgctat atttttaacc
     7741 attttgatgt ttccccttat tcagggcttt atccaaactc acggactatt ggagagcgct
     7801 tacaatttct ttaccacgcg caaaactcta aacccggaga
//
LOCUS       DATBTE010000262         7924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_581157, whole genome shotgun sequence.
ACCESSION   DATBTE010000262 DATBTE010000000
VERSION     DATBTE010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 7924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7924
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581157"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1702
                     /locus_tag="VIG33_09770"
     CDS             <1..1702
                     /locus_tag="VIG33_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY8271162.1"
                     /translation="KFGQFYHQVLDLNPAAQWIAVVPEDFAPEQLADIHELYPLFRVI
                     SSYHETDIEVYFFSALEEVNRQKQDKNLEKLIREQKDKLIRLQKDLEERVEKRTKFLT
                     EARRKLYQTNVRIEGLKNALMAVHQAGSLVEIEKLLNESMASTVQTSWIRIFFAPQDN
                     LFADQVETKLNFTQQRIPLFRQHERIGSIFFMRAADHPFLRDEVDFLNRVAEAVSLAL
                     GRIQKLEESEELKKQWEATFNAVSDPVAIIDANYNIIQANSAIASQHSVGSVMGKKCF
                     EILYGRQSPCEHCQRGKNFRLTSQDGKMMKSLDVYSQSLSLELQKPAVYVNIYHDITQ
                     RLQMEKQLLETAKLAELGTIGSSIAHELNNPLGGILSFTQLIKMDLPKDHPLYSDIVE
                     MEAGALRCKDIIQNLLGFTRNPNVDEIADLDLRDVIKRAYKIMDLQIKSQAIEVILNL
                     PDKNCPARGHLNLLSQAIKNVLQMAFDSIHSRTKQNSKTFKGQLEVNLQLTTDKYLIT
                     VKNNGIQYEKESAGLGIPVASQIVEDHGGRLEIFSSTNPETGLITQAKISFPRPVLRP
                     "
     gene            1794..3197
                     /locus_tag="VIG33_09775"
     CDS             1794..3197
                     /locus_tag="VIG33_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8271163.1"
                     /translation="MRNHRVLILDDESSLRTALFRVLDRKGLNVITANRIEEAKLLCQ
                     GDTVIDLAIVDLNLPDGDGIEFMTFLKLLYPAIEVVILTGHATIEAAVRATQKGAFHF
                     VTKPFSLDELISLVDRALAHKHLQQENHQLRSELHRKYKFDQIIGNSEQIQSVLRMVE
                     RVADSDSTVLVHGETGTGKELIARAIHYNSPRAKGPFVPINCGAIPGELLESELFGHI
                     KGAFTGAIANRVGRFEMADGGTIFLDEIGDMDPTLQVKLLRALQEKSFEPVGSTKTVH
                     VNVRVITATNVNLEQAVEMGRFREDLFYRLNVIPINIPALRERKSDIPLLLRHFMDAF
                     GKNKGRSLTGFSKEALDCLVHYPWPGNIRELENLMERMTILKGEGQLEVVDLPLKYKA
                     GKTLSQEASVVDIPEVGLDFNTAVDSYENILILKALEKTGWNRNQAAILLKLNRTTLV
                     EKIKKKGLRPPEGTIEV"
     gene            complement(3194..3565)
                     /locus_tag="VIG33_09780"
     CDS             complement(3194..3565)
                     /locus_tag="VIG33_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271164.1"
                     /translation="MKNMMVLTMLLLGTTSAFAHIENARVCSIGRDGDVYTSDFRVVG
                     SSVIWNQDGSDLNYNVESVKNSSLSKASAACGENVVQATKYVLSSLIGITDLELAIGV
                     SGTQYMIFPDVGVGASSDNCD"
     gene            complement(3758..4822)
                     /locus_tag="VIG33_09785"
     CDS             complement(3758..4822)
                     /locus_tag="VIG33_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271165.1"
                     /translation="MKTQYFYPQRLLASFAALVALSCAKTNFIPADLGQEATSITSPG
                     KVVNSTEIVASGNKEVDFLLVLDDSNSMLPELKKLSSRMATFVKFLEASQIDWQMCVT
                     TTRSPNFGKYLEWKNYTPPAGVPAYVLKKGATGLNTIFPSTINSVSIGGGDSGDERAI
                     KSSYVSFQNGGPCYRPGAAVSVIAISDEDERSVGGDSKNLKSKEKPSSFLPLENEDLP
                     MNLKARAQDSFGVEVRFTFNSIIVKPGDKQCEATQDVDVSPCHPGVLYSMMSGLTEGG
                     VGSICDADYSSNLNTFKDKIVNSLNQLTLQCDPVKETLRVSVNQNLVSDFKLEKRLLK
                     FGKDLPEGTRIDLTYECHEE"
     gene            complement(4989..5927)
                     /gene="speB"
                     /locus_tag="VIG33_09790"
     CDS             complement(4989..5927)
                     /gene="speB"
                     /locus_tag="VIG33_09790"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY8271166.1"
                     /translation="MDYKPLSGREFPRFSAIKTFFRLPYVPVDADYEVGIFGIPYDGG
                     ASYRPGPRFAPTAVREISSLGRGFHMTRELNWIEKLKVADIGDCSTVPIDQVQTYALI
                     EKYVSEILGQKKKFIACGGDHSITLPVLRALRKYYGCPLQFIHFDAHLDTYPAAWGCE
                     YHHGAFARHAVEEGLVDPKKMWQIGIRGPLAAGSDLDFVNKQGIRVFTMDDLREGPLN
                     DFLTQLPTCDETPTYISFDVDCLDPAFAPGTGTPVPGGMTSYEAQRILRALKIPNLVG
                     GDVVEISPPFDHAGITSLIGVDVMFEFLCIMAGNKK"
     gene            6072..7526
                     /locus_tag="VIG33_09795"
     CDS             6072..7526
                     /locus_tag="VIG33_09795"
                     /inference="COORDINATES: protein motif:HMM:NF042614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6798 domain-containing protein"
                     /protein_id="HEY8271167.1"
                     /translation="MFYFVFFLFILIPVLRLGYHFGNLDHTILSLQGIAKARPTAFLN
                     DWFIKNAPQPHWHFDLLTQAGESLGLLHEFYLLYWFFSVGILVCALKLICQHLKIEKA
                     LPWIALILLLGPWRFLGSGPLWGSWALPHFMGGSLGLLACALWLQKKEKWLWFVVPLA
                     TLFHIQHGALVSLLLVSGSLFQWRKNLPVICLAILNLGGVFAAAKLRGLTDSGNAFLM
                     SCQKYIPEHCFAPAWPLDWLLPLVVIFIVFIFLGKKIWRDGGDSWTALALSASCITFF
                     GTLIDYFDIEPLATLFRRLFFHRVAAYSLCFAVVIIAQMIWEGDLRNLQNKTLKIFAA
                     ISIVILLISPSGILGEKADLRKLTANQPVYEILGHHLETLVPPGEIIAMNPALVWVRL
                     LSRRAVVVDVKAIPFQDQPFKEWEERIRDLGGFDFDGNKFKQMSFYQLKFMSQKYKAS
                     FVLLEIDDQKLENAKKEFRFVATVGGIFVLFSTQ"
     gene            7584..>7924
                     /locus_tag="VIG33_09800"
     CDS             7584..>7924
                     /locus_tag="VIG33_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271168.1"
                     /translation="MKLNVFFGSALISILTLFTLTACKTIDINEGRVPSEYLAQAKQL
                     AGTYKGSFNGVAGDLVISFNGDKPVVKYHNSHGDDILNNNCHSSFGDLLQVTVKNERK
                     NPSVSRALFDF"
BASE COUNT         2215 a         1890 c         1687 g         2132 t
ORIGIN      
        1 aaaattcggt caattctatc accaagttct ggatctgaat cccgcagctc agtggatcgc
       61 agtggtgccc gaggatttcg ccccggaaca actggcggat atccatgaac tctatccact
      121 ctttcgagtt atttcttcct atcatgaaac cgatatcgaa gtttattttt ttagcgcgct
      181 ggaagaggtg aaccgccaga aacaagacaa gaacctcgag aaactcattc gagagcaaaa
      241 agacaaactc attcgcttgc aaaaagacct tgaagaacgc gtcgagaagc gaaccaaatt
      301 cttaaccgaa gctcgccgca aactctatca aaccaatgtg cgcattgaag gactcaaaaa
      361 tgcactcatg gccgtccatc aagccggctc cttggtggag attgaaaaac tcttaaatga
      421 gtccatggct tcgacagttc agacttcgtg gattcgcatc ttctttgcgc ctcaggacaa
      481 tctctttgca gatcaggtcg aaactaagct gaactttacc caacaacgca tccctttatt
      541 tcgacagcac gagcgcatcg ggtctatttt ctttatgcga gctgcggatc atcctttttt
      601 gcgtgatgag gtggattttt tgaaccgtgt agcagaagcg gtttccttgg ctctgggacg
      661 gatccagaaa ctcgaagaat cagaggaact caaaaagcaa tgggaagcca ccttcaacgc
      721 cgtctctgat ccagtggcta tcatcgatgc caactacaat attattcagg cgaactctgc
      781 catcgccagc caacactccg tcggcagtgt catgggaaaa aaatgttttg agattctcta
      841 tggtcgccaa agcccttgcg aacattgcca gaggggtaag aatttccgcc ttacctctca
      901 agacggcaaa atgatgaagt ccttagatgt gtatagccaa agcctctcct tggaacttca
      961 gaaaccggca gtttatgtaa atatctatca cgacatcaca caaagactac aaatggaaaa
     1021 gcaacttctg gaaaccgcca agctggcgga gctggggacc atcggcagca gcatagccca
     1081 tgaactcaac aatcctttag ggggaatttt gtctttcact caattaatta aaatggacct
     1141 ccctaaagat catccgcttt attctgacat tgttgaaatg gaagcgggcg ctcttcgatg
     1201 caaggacatt attcaaaatc ttctaggctt cacgcgaaat ccgaacgtcg atgaaatcgc
     1261 tgatctcgac cttcgcgatg tcatcaaacg tgcgtacaaa attatggacc tgcaaatcaa
     1321 gtcacaagct attgaagtta ttttaaatct gccagacaaa aattgtcccg cccgaggaca
     1381 tttgaatctt ctctcgcaag cgatcaaaaa tgttctgcaa atggcttttg attccattca
     1441 ctcccgcact aaacaaaatt ccaaaacttt caaaggccag ctcgaggtca acttgcaatt
     1501 gacgacagac aagtatctca ttacggttaa aaataatggg attcaatatg aaaaagaaag
     1561 cgcgggtctt ggcattccgg tggcctcgca aatcgtcgag gatcacggcg gtcgtttaga
     1621 aattttttcc tcgaccaacc ccgagactgg tcttatcact caggcaaaaa tttccttccc
     1681 tcgtccagtt ttaaggcctt aaaatacaat gcccacgcgg attttttgac agtcctgtca
     1741 gaaatatatt actctaatta aagaaacgag aacccaccaa ggaaggaagg ttcatgcgca
     1801 atcatcgcgt gcttattctg gatgatgaat caagcttacg tacagctctc ttcagggtgt
     1861 tagacagaaa aggacttaat gtcatcaccg ccaaccgcat cgaagaggcc aagctcctgt
     1921 gtcagggtga tactgtcatt gatttagcaa ttgtagatct gaatcttccc gatggggacg
     1981 gcattgaatt catgactttt ttaaaattgc tctatccagc gattgaagtg gttattttaa
     2041 cgggtcacgc caccattgag gccgccgtgc gtgccactca aaaaggggcc tttcactttg
     2101 tcacaaaacc gttcagcctc gacgaactga tcagcttggt cgacagagcc ttggcgcata
     2161 aacatctgca acaggaaaac caccaactgc gcagtgaact tcatcgtaag tataaatttg
     2221 atcaaatcat cggcaacagt gaacagattc aaagtgtctt gcgtatggtc gagcgcgtgg
     2281 cggactcaga ctccacagtt ctggtccacg gtgaaactgg aacaggcaaa gaactcattg
     2341 cgcgcgccat tcactacaat tctccaaggg ccaaggggcc tttcgtaccc atcaattgtg
     2401 gcgccattcc cggtgaactt ttagagagtg aacttttcgg tcatatcaaa ggtgctttca
     2461 ccggcgctat tgccaatcgc gtgggccgtt ttgaaatggc tgacggtggc acaatcttcc
     2521 tggacgaaat cggcgatatg gatccgaccc tacaggtaaa actacttcgc gcacttcaag
     2581 aaaaaagttt tgagccggtg ggcagtacta aaaccgtcca tgtaaatgtc cgcgttatca
     2641 ccgcaacaaa tgtgaatttg gaacaagccg ttgaaatggg tcgcttccgc gaagacttat
     2701 tctatcgctt gaatgtcatt cctataaata tcccagcctt gcgtgaaaga aaaagcgaca
     2761 tccccttgct actccgccac ttcatggacg cttttggaaa aaataaaggg cgcagtctca
     2821 cagggttttc caaagaagct ttggattgcc tcgttcatta tccctggccc ggaaatatcc
     2881 gagagctgga aaacttaatg gaaagaatga ccatcttaaa gggagaaggc caactggaag
     2941 tggtggattt accgctgaaa tacaaagctg gcaaaacctt gagccaagaa gccagcgtcg
     3001 tggatatccc tgaagtcggc ttggatttca ataccgccgt ggactcttat gaaaatatcc
     3061 tgatcttaaa agccttggaa aaaacaggct ggaatcgaaa tcaagccgcc atcctactga
     3121 aattgaatcg caccacactg gtggaaaaaa ttaaaaagaa aggtctgcgt ccaccagaag
     3181 gaacgattga agtttaatcg cagttatcag aacttgcacc aacgccaaca tccgggaata
     3241 tcatatattg ggttcccgaa acacctatag cgagttcaag atcggttatt ccaatcagag
     3301 agctgagaac atattttgta gcctggacta cattttcgcc gcaagcggcg gaagctttgc
     3361 tcaggctcga attcttaaca gactctacat tgtaattcag gtcgctgccg tcctgattcc
     3421 aaattacact tgagccaacc acacgaaaat cactcgtata aacatcccca tcgcgaccaa
     3481 tactgcaaac cctcgcattt tcaatatgcg caaatgccga agtcgtgcca agcaatagca
     3541 ttgttaaaac catcatgttt ttcataagac ctccaagtag aataaatcga aatacttaat
     3601 cgaccgcatc tactacaaga gcaaacgacg ggccaggctt tacaaagata atcacccctt
     3661 aattgcgact taacaaaaaa agcaaatgat aaaagtgccg caaaccgtct ggtgtaaagg
     3721 acttcgacag attcagcgga ccgtccgccc ctggccctca ctcctcatgg cactcataag
     3781 ttaaatcaat gcgagttccc tcgggaaggt ctttaccaaa cttcaaaagt cttttttcaa
     3841 gcttaaagtc tgaaaccaaa ttctgattca ccgatactct gagggtttct ttgaccggat
     3901 cgcactgcaa agtgagttgg ttgagtgagt tcacgatctt atctttaaaa gtgttcagat
     3961 tcgagctgta atccgcatca cagatgctgc ccacgccgcc ttccgtgaga cccgacatca
     4021 ttgagtaaag aacaccagga tgacatggcg aaacgtccac gtcttgagtg gcttcacatt
     4081 gcttgtcgcc aggttttaca atgattgagt taaaggtgaa acgcacttcc acgccaaagg
     4141 aatcttgagc acgggcctta agattcatgg gaagatcttc attctcaaga ggtaaaaaag
     4201 aggatggctt ctctttcgac tttaagtttt tcgaatcgcc accgacagag cgctcgtctt
     4261 catcagaaat ggcaatcaca gaaaccgcag ccccgggacg atagcaaggt ccgccatttt
     4321 ggaaactcac atacgaggac ttaatagctc tctcatcacc ggagtctcct cctccgatgc
     4381 tcacagaatt aattgtagaa ggaaaaatcg tgttcaatcc tgtggctcct ttttttagaa
     4441 cgtaagcggg aactcccgca ggcggagtat aatttttcca ttccaaatac tttccaaaat
     4501 ttggagaccg ggtcgtggtc acacacattt gccaatcaat ttggctggcc tccaaaaatt
     4561 tcacaaaagt ggccatgcgg gaagaaagtt ttttgagctc cggcaacatc gagtttgaat
     4621 catcaagaac cagaaggaaa tccacctctt tatttcctga agccacaatc tctgtggaat
     4681 tcacaacttt accgggggaa gtgatgctcg tagcttcttg ccctaaatct gcgggaataa
     4741 aatttgtttt cgcgcaagag agagcaacaa gagctgcgaa gctggccaaa agtctttgtg
     4801 gataaaagta ttgtgttttc atacctgtct tttcgtcagg tccagaaaaa acttttgctc
     4861 aaaatgaaaa aaattgaaaa acaggagatg ccctcaactt aagcactgca acattactct
     4921 tatttgtgta aattataggc actattacga ccttggtcca gctcgctcta tacattttta
     4981 tttttcattt attttttatt ccccgccatt atacagagga actcaaacat cacgtccacg
     5041 ccaataagag aagtaatacc cgcatggtca aagggcgggg aaatttcgac cacatctccg
     5101 cccacaagat tcggtatttt caacgcacgc aaaattcttt gcgcttcata gctcgtcatt
     5161 ccccctggaa caggagtccc cgttcctgga gcaaaggctg ggtcgagaca gtcaacgtca
     5221 aaactaatat aggtgggagt ctcatcgcag gtgggcaact gtgttaaaaa atcattcaga
     5281 ggtccttcgc gaaggtcatc catggtaaag acccggatcc cctgcttatt tacaaaatca
     5341 agatcgcttc cggcagccag aggtcccctg attccaattt gccacatttt ttttggatcc
     5401 actaagccct cttccaccgc gtggcgggcg aaagcgccat gatggtattc acaaccccag
     5461 gctgctggat acgtatctag atgagcatca aagtggataa actgaagagg gcagccgtaa
     5521 tattttctaa gcgcacgtaa aactggcaaa gttatggaat gatctcctcc acaggcgatg
     5581 aactttttct tttgtcctaa aatttcgctc acatattttt caataagcgc ataggtctga
     5641 acttgatcaa tgggcaccgt ggagcaatct ccaatatcag cgactttgag cttctcaatc
     5701 caattcaatt cgcgagtcat atgaaaaccc cggcctaagc tggaaatttc cctgaccgct
     5761 gtcggggcaa aacgaggccc tggtcgataa gacgctcccc catcataagg gattccaaaa
     5821 atacccactt cataatccgc atcaacagga acatagggca atcggaagaa cgttttaatc
     5881 gctgaaaacc gagggaactc acgcccactg agaggtttat aatccatgac tctcctcttt
     5941 acaaattcta gttcttttta cttctcgtag tatctgacag aaagctcttt gaaacaacgg
     6001 ctttatcaaa agttgacctg ccagtgggaa aactgaataa ttgtcagaaa gagaatagag
     6061 ccggaggcct tttgttttac tttgtcttct ttctatttat tttgattcca gttctacgtt
     6121 taggctatca cttcggaaat cttgatcata cgatcttgag tcttcagggc attgccaagg
     6181 ccaggccgac tgccttcctg aacgactggt tcataaaaaa tgcgccccaa ccgcattggc
     6241 attttgatct cttaacgcaa gccggagaat ctcttgggct cttgcatgag ttttatttac
     6301 tttactggtt tttctcagtc ggtattttgg tctgcgcgct gaaactcatt tgccaacatc
     6361 ttaagattga aaaagcactt ccctggatag ccctcattct gttgctggga ccttggcgct
     6421 tcctggggag tggtcctctg tggggctctt gggcccttcc tcactttatg ggaggatcgc
     6481 tgggccttct tgcctgtgct ttatggttac aaaaaaagga aaaatggctt tggtttgtag
     6541 tccctttagc gactcttttt catatccagc atggagccct cgtctctttg cttttggtca
     6601 gtggaagcct ttttcaatgg cgcaagaatc ttccggttat ttgcttagcg attttgaatc
     6661 tgggtggcgt ctttgcggcc gcgaaactac gtgggcttac agacagtgga aacgcttttt
     6721 taatgagctg tcaaaagtat attcctgagc actgtttcgc ccccgcttgg cccttggatt
     6781 ggctgcttcc cttggtggtt attttcatcg tttttatatt cttgggaaaa aagatttgga
     6841 gagacggggg cgattcctgg acagccttgg ccttatcggc aagctgcatc actttttttg
     6901 gcaccctcat tgattatttt gatatagaac ctttagccac tctttttaga agactttttt
     6961 ttcatagagt cgctgcctac agcctttgtt ttgcggtggt gatcattgcc caaatgattt
     7021 gggaggggga ccttcgaaac ctgcagaata aaacactcaa aatttttgcg gctatctcta
     7081 tcgtgatttt gctgatctcc ccctcaggca ttttaggtga aaaggcggat ctgcgcaagc
     7141 tcaccgccaa tcaacccgtc tatgaaattc taggacatca tcttgaaact ctcgtgccac
     7201 ccggggaaat cattgctatg aaccctgctc tggtttgggt tcgattgctt tctcgccgcg
     7261 ctgttgttgt tgatgtaaaa gccatcccgt ttcaggatca gccatttaaa gaatgggaag
     7321 aacgcattcg ggacctcggc ggatttgatt ttgacggaaa taaatttaag caaatgagtt
     7381 tttatcaact taaatttatg tctcagaaat acaaagccag ttttgttctt ttggaaatcg
     7441 atgatcaaaa attagaaaac gctaaaaaag aattccgttt tgtagccacc gtcggcggca
     7501 tatttgttct ttttagcaca caataattaa gttgatatcg acctctcttt gagattatct
     7561 tcaggcataa ctggaggttt taaatgaaat tgaatgtctt ttttggctct gccctgattt
     7621 ctatcctgac acttttcacc ctcaccgctt gcaaaacaat tgatatcaac gagggccgtg
     7681 tccccagcga atatctggct caagcaaaac aactcgctgg cacctacaaa ggctccttca
     7741 atggagtcgc cggagatctc gtcatcagtt tcaatggcga caaacctgtg gtgaaatatc
     7801 ataatagcca tggcgatgat atcttaaata ataactgcca ctcttcgttt ggtgatcttc
     7861 tgcaagtaac ggtaaaaaat gaacgcaaaa atccctctgt gagcagggcg ctttttgatt
     7921 tcga
//
LOCUS       DATBTE010000263         3330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2426899, whole genome shotgun sequence.
ACCESSION   DATBTE010000263 DATBTE010000000
VERSION     DATBTE010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2426899"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1715
                     /gene="nosZ"
                     /locus_tag="VIG33_09805"
     CDS             <1..1715
                     /gene="nosZ"
                     /locus_tag="VIG33_09805"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR04246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HEY8271169.1"
                     /translation="PQTSYGFSEETKPLLETSHGFVPWDDSHHPWLSQSNGQDDGRWL
                     FINSNNTPRIARIDLSTFKTVEMLEIPNSAGNHGSPFGTMNSEYLVASTRFSVPIPQK
                     DIPIKNVEEMKGSISFIKVEPGSGHMSIAFQLIAPGINYDLAHCGKGPSHGWCFFTSY
                     NSEKAHTLLEVKASKNDKDFIAAVNWKMLEQCVAKGKTKDLKGDFNHSLYEDATHSAK
                     VEKLKSVKTLTPENCPGSIYYIPVAKSPHGSDVDDTGEYFVAGGKLAAQITAYSFKKM
                     EEAIKNNKFTGDFEGVKILDYASVMAGEVKKPCLGPLHTEFDGKGFAYTSCFVSSEVV
                     KWKVGTWEVVDRMPTYYSLGHLMIPGGNTMKPWGKYLFAMDKITKDRYLPTGPELNQA
                     AQLLDISGKKMEMLLDFPTIGEPHYAQGIAADLVIKGQKRIYKLEDNKHKYVTKNESE
                     VKVVRNGKDVHVYMISIRSHFAPDNIEGIRVGDNVYFHVTNLEQDWDIPHGFSVIGAN
                     TAELLIMPGETSTFKWTPKAVGVVPFYCTDFCSALHQEMQGYIRVSPATSKTPLKWGL
                     AKAL"
     gene            1791..2381
                     /locus_tag="VIG33_09810"
     CDS             1791..2381
                     /locus_tag="VIG33_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271170.1"
                     /translation="MKNLFFGRISLLSRSLVVVASLLLLLTYFFPMWRISLWAPQYPE
                     GLSLFIWAHKLSGDIQTVNILNHYIGMAHIEDASFPELQLFPKIFTGLWIMGLLVAAI
                     GRKWGLYLFAISNLSFAGWAMVDFYNWEYQFGHNLNPEAAIKMEDMVYQPPLIGEKLF
                     LNITASSWPDIAGFAFSATVAIAVVVSVMELLRKDA"
     gene            2374..2832
                     /locus_tag="VIG33_09815"
     CDS             2374..2832
                     /locus_tag="VIG33_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271171.1"
                     /translation="MPRSILVTLSLTLMAFGIVSCTSKKSEPIYYGKDSCSHCKMLIV
                     DKRFGAEIVTAKGKIFKFDSIECMHHFEVTENEKLGSHFAEYLANTEKQGELMLAEDA
                     YICEDPTVHSPMGKGYLTADSKEKIKIVFAKNDKACPVFRWNELTNKLGK"
     gene            2869..>3330
                     /locus_tag="VIG33_09820"
     CDS             2869..>3330
                     /locus_tag="VIG33_09820"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HEY8271172.1"
                     /translation="MFIFRFSKIFAFSFGLLLINGPVASQQLIEVCASCQYRTLKSAI
                     AAATAGATIKVKEGVYKENRIQIDKKLSLVAEGKVIVDANGEGHGFILYKTQNVSITG
                     FTIQNTGMSYTEEFSGVRAIESKDCTIASNTFLDTTYAVYLENSEACLVADN"
BASE COUNT          924 a          648 c          768 g          990 t
ORIGIN      
        1 atccccaaac tagttatggc ttctcggaag agaccaagcc tttgttggag acttcccacg
       61 gatttgtacc ctgggatgat tcccatcacc catggctttc tcagtcaaac ggccaagatg
      121 atggtcgctg gttattcatc aactctaata acacgcctcg gatcgctcgt attgatctta
      181 gcacttttaa gacggtcgag atgcttgaga ttccgaattc cgctggaaat catgggtccc
      241 ccttcggaac aatgaacagc gagtacctgg ttgcttcaac gcggttttct gtgcctatcc
      301 cacagaagga tataccaatt aaaaatgtgg aagagatgaa aggttcgatc agctttatca
      361 aagtcgagcc gggctctggt cacatgagta tcgcatttca attgattgct ccggggatca
      421 attatgatct tgcgcattgc ggaaaaggac cttcccatgg ttggtgcttc ttcacttcgt
      481 acaactctga aaaggctcat actttgttag aagtgaaagc ttccaagaac gataaagact
      541 ttatcgctgc ggttaattgg aaaatgcttg agcagtgtgt ggccaaaggt aaaacgaaag
      601 acttgaaagg tgattttaat cacagtcttt atgaagatgc gactcactcc gcaaaagttg
      661 aaaaactcaa gtctgtgaaa actttgactc ccgagaactg cccaggatct atttattata
      721 tcccagttgc aaaatctccg catggctctg atgttgacga tactggtgag tactttgtcg
      781 ctggcgggaa attggctgcg caaattaccg cttattcctt caaaaagatg gaagaggcta
      841 ttaagaacaa taaattcact ggtgactttg aaggtgtgaa aatccttgat tacgcttcgg
      901 ttatggctgg ggaagtgaaa aaaccatgtc ttgggccgtt gcatacggag ttcgatggaa
      961 aaggcttcgc ttacacttct tgtttcgtga gttctgaagt tgtgaagtgg aaagtgggaa
     1021 cctgggaagt ggtggaccgc atgcctactt actactcttt gggtcatttg atgattccag
     1081 gcggaaatac catgaagccc tggggtaagt atcttttcgc catggataaa atcactaaag
     1141 atcgatattt gccaactggt cccgaactga atcaagcggc tcagttgtta gatatttccg
     1201 gaaaaaaaat ggaaatgctt ttggatttcc cgaccattgg tgagccccat tacgcacaag
     1261 ggattgcggc tgacttagtg ataaaagggc aaaaacgtat ttataagtta gaggataaca
     1321 aacataaata tgtgactaaa aatgaaagtg aagtaaaggt tgttagaaat ggaaaagatg
     1381 ttcatgtata catgatttcg atccgttctc acttcgctcc ggataatatt gaaggaatca
     1441 gagtcggtga caatgtttac ttccatgtca cgaaccttga acaagattgg gatattccac
     1501 atggattttc agttattggc gcaaacacgg cggaattact gattatgcct ggtgagacat
     1561 ccacttttaa gtggacacca aaagcggtag gagttgtgcc tttctattgc actgacttct
     1621 gctccgctct tcatcaggaa atgcaaggtt atatccgtgt ttctcccgcg acgtcgaaga
     1681 cgccgttgaa gtggggtttg gctaaagctt tatagtttta tattgatgtt ttgaaaagtt
     1741 caattgtgag cacaaactca caattggact cttatcgcga gaggatttcc ttgaagaacc
     1801 tgttttttgg aagaatttct ttattatccc ggagccttgt ggttgtggcc tcactattac
     1861 tgcttctcac ttattttttt ccgatgtgga gaatttctct ttgggctccg cagtatcccg
     1921 aagggctcag tttatttatt tgggcacata agctttctgg tgatatccaa acggttaata
     1981 ttttaaatca ctatattgga atggctcata ttgaagatgc cagttttccg gagcttcagt
     2041 tgttcccgaa gattttcact ggactttgga ttatgggatt gctggtcgct gccattggac
     2101 gaaaatgggg tttgtattta tttgcaattt caaatttgag ctttgctgga tgggccatgg
     2161 tggactttta taattgggaa tatcagttcg gacacaacct gaatcctgag gctgcgatta
     2221 aaatggagga tatggtttat caacctccgc ttattgggga aaaattgttc ttgaacatca
     2281 ccgcctcttc ttggccggac atcgcgggtt ttgctttttc cgcaaccgta gctattgctg
     2341 tcgttgtgtc agtgatggaa ttattgagga aagatgccta gatccatact tgtgacttta
     2401 tctctcactt tgatggcctt tggaattgtc agttgcactt caaaaaaatc agagcccatc
     2461 tattatggta aggactcttg ctcccactgc aagatgttga ttgtcgacaa acgttttggg
     2521 gccgagattg ttacggctaa gggaaagatc ttcaagtttg attctatcga gtgtatgcat
     2581 cattttgaag tcactgaaaa cgaaaaatta ggatctcatt ttgccgaata tcttgccaat
     2641 acagaaaaac agggagaatt gatgctagcg gaagatgctt atatttgtga agatccgaca
     2701 gtgcatagtc ctatggggaa aggttatttg actgcggatt cgaaggaaaa aataaaaatc
     2761 gtctttgcta aaaatgataa agcctgccct gtttttcgct ggaatgaact caccaacaag
     2821 ttgggaaaat aatatcggaa tgctatgatt agatttatat ccaggtttat gtttatattc
     2881 agatttagta aaatcttcgc attttctttc ggacttcttt taataaatgg tcctgtggcc
     2941 agccaacagc tcattgaagt ttgcgcgagt tgccagtacc gcactctgaa gtccgccata
     3001 gcagccgcta cagcgggagc tacaatcaaa gttaaagagg gagtttataa agagaaccga
     3061 attcagatcg acaaaaagtt aagccttgtc gctgaaggaa aggttatcgt tgatgccaat
     3121 ggcgaggggc atggattcat cctgtacaaa acacaaaatg tttcaatcac gggctttacg
     3181 attcagaaca ccggcatgag ttataccgaa gaattttcag gcgtacgagc gatcgaatcc
     3241 aaggactgca ctattgcctc aaatacattt ctagacacca cctatgccgt ttatctggaa
     3301 aactccgaag cctgtcttgt tgcggataat
//
LOCUS       DATBTE010000264         3981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1674459, whole genome shotgun sequence.
ACCESSION   DATBTE010000264 DATBTE010000000
VERSION     DATBTE010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674459"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..703
                     /locus_tag="VIG33_09825"
     CDS             185..703
                     /locus_tag="VIG33_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8271173.1"
                     /translation="MRQIDFGFFKKQSSSFGGSSLLGKRKTAHPLSTKKPLHLVLKSE
                     SSSLFNPGNRILEKIIRDHAKKYSIAVYALSFNWSHIHLVIKLPTRESYRAFIRTVTA
                     ALLKCLAKQKGKSLKGVFDLRPFTRILNWGRDLRNVINYHELNDLEGWGLVSRKKKKH
                     PPPKPSNFKKTG"
     gene            complement(1814..2047)
                     /locus_tag="VIG33_09830"
     CDS             complement(1814..2047)
                     /locus_tag="VIG33_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271174.1"
                     /translation="MKYLLLCTLSFLNFARAIEPSGDLLKESLVLLRANGSCGTGFVL
                     SSGKIITNSHVIDDKCNQLHCPDVEISQANAID"
     gene            complement(2108..2692)
                     /locus_tag="VIG33_09835"
     CDS             complement(2108..2692)
                     /locus_tag="VIG33_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271175.1"
                     /translation="MNQNFLFGFFLPFLVVPAMALPCHDFAASNIEKTEKVYPLQLLN
                     LHKQGAEIPNMKFSGPGICYAISMSGQGSEEKDFIYDVLGNGKGGGQFTYLKKEGRKE
                     AMLFRFNSYPYEEEACQLNLKRDSQFASHAIATSYINQYNSSTHLYLYQSSRSAMEYP
                     EEREIRMVAGGKGLTVHIVSPYSLRYTICYFPKR"
     gene            complement(2773..2904)
                     /locus_tag="VIG33_09840"
     CDS             complement(2773..2904)
                     /locus_tag="VIG33_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271176.1"
                     /translation="MSLIIFFGGVSVVVLIAIGVVVHGVYHHAQEAQKLKLDKKDSL"
     gene            complement(2901..3743)
                     /locus_tag="VIG33_09845"
     CDS             complement(2901..3743)
                     /locus_tag="VIG33_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEY8271177.1"
                     /translation="MMDNSLTPQGPLQPEESKYVLHRRFDRMGRLIGDAAMLKLFKSH
                     VMIIGVGGVGSWAAESLARSGVGKITLVDFDEVCITNANRQLHAIQGMVGKKKAEVMG
                     ERLRKINPQMVVEVVPVFYNAENSEMILAKKPDYIVDAIDNLTAKAHLLNTCRQQNLK
                     VITSAGAAARLDPMQIKRADLADTYGDPMAQQLRKILRQKHDWPAKGPFGLQCVFSDE
                     EPIQPIELHYDNGEGFKCVCPQNQNNLHSCEHRNVIWGTASFVTGAFGLNIASWIVRE
                     VVNS"
     gene            complement(3733..>3981)
                     /locus_tag="VIG33_09850"
     CDS             complement(3733..>3981)
                     /locus_tag="VIG33_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY8271178.1"
                     /translation="GGPVVWEQNKKLHHVIRDLPLEFLLLESDSPDQPPPRFKQSLNQ
                     PASLWDVAEKIGELREMQPTEILEIATTNFKRLFHYDG"
BASE COUNT         1202 a          889 c          820 g         1070 t
ORIGIN      
        1 ggggctatat aggacgaaat aatgacggtc caccaggaat gatctctgtt tggagaggat
       61 ggatggtgct gatgcaatcg gaaaaaacat ggaggtcaat tgaagctttt aattgtgggt
      121 aaagataagc ctttaaaaac ccttgcaaaa acccatgcta taaccttctt ccggaggtct
      181 ccttttgcga caaattgatt ttggtttctt taaaaaacaa agctcaagct ttggcggaag
      241 ctctctcttg ggcaaaagaa aaacagctca tcctctttct acaaaaaagc ccctgcatct
      301 ggtgcttaaa agcgagagct cttccttgtt taacccaggt aaccgtattc tagaaaagat
      361 aatcagagat catgccaaaa aatattccat tgctgtttac gctctttcct tcaattggag
      421 tcatattcac ttagtaataa agcttcccac ccgagaaagt tatcgggcct ttatcagaac
      481 cgtcacggca gctctcctga agtgtcttgc aaagcaaaag ggaaagagcc ttaaaggagt
      541 ttttgatctt cgccccttca ctcgcatctt aaactgggga cgcgatttac gaaatgtcat
      601 aaattaccac gaactgaatg atctcgaagg ctggggactt gtctcgcgaa aaaagaaaaa
      661 acatccacca cccaagccct ccaactttaa gaaaaccggt tagaaagagg aaacgcattg
      721 gcttttttta aaaagctaag actactgatc atctacttat tccccaaaaa gccaccatca
      781 ttcacataac ttagtgagca aagaaaataa gaattttctt aaaacgaatg aggcattttc
      841 tagtgattca ttatgagccg catcttttaa cgctgcggca ttaaagtgac gaacaagccc
      901 tagtttttaa attccaagga tccaccaaag aagcacagta gaaagaaaac tcaagaggag
      961 ggagccaatg agtattttct tgaacagata gaatcgattg gccaagaaat aaaataagaa
     1021 aaacaaaatg aacaggaaca tcatagatgt aacgaaaacc tttgcgagga aagcataggt
     1081 ctcaatctcc catctaagct tcttaacgag cgaattctca ctctggtata tcttggaagt
     1141 ttggagaaat tgttcttccg cgaaataggc cataggggaa agcgttttta gcgcatcgag
     1201 tttatcgcga taagagctag aaaagaacgc tttgtgatcg accaggattt tataagagtg
     1261 gtgttcaaca ctttcttgaa agagttcata acactcttgt gatagggact ttgtatcaat
     1321 atctggtggg ggaacgttca taggaaaatt ggcgtgaatc caaatccaat agtcttttaa
     1381 ttgtggacac tgtccgctta aggctacttg cgcttcaata tcttgaatgt ggagtttccg
     1441 cccaaaagag gctatttcaa agtacctttc gcttgtcgtc gcaaccctga aataagaagc
     1501 atacagtttt ttcaagtcga tgagaccact ttttgcagcg atcgcctcaa ggtccgttcc
     1561 gaggtgatcg attcgagtcc ctaaacccag atcatcgtca ttagttttat aggcaaggcc
     1621 gattatgtcc gaattcttgt tgaaaatcgg gctgccactc tgtcccttat aaatggaaat
     1681 ccctagtcca aattcatggt ccgttacttg agtgatgtcg cctgtagtaa tttgaagaga
     1741 ttcacaggaa ggaaagccaa tggtgaaaaa actttctttg tctaggggcg ccgcaatctt
     1801 tccagaaagc cccctaatct atagcgttgg cttgggatat ctccacatct ggacaatgga
     1861 gctgattaca tttgtcatca atcacgtgtg aattagtgat gattttccca gaagaaagaa
     1921 caaaaccagt tccacagctg ccattggctc gcaagagcac aagagattct tttaaaagat
     1981 cgccagaggg ctctatggct cgggcaaaat ttaaaaaact aagagtgcaa agcagcaagt
     2041 atttcatccg cagtcagata aaacataaaa cccctatttt caatagggat tttcatcaat
     2101 acgtgcttta tctctttgga aaataacaaa tagtgtaacg aagagaataa ggactcacaa
     2161 tatgaacagt gaggcccttg cctcccgcaa ccatgcgaat ttctcgttct tcgggatact
     2221 ccatcgcact gcgactgctc tggtataggt atagatgtgt cgaagaattg tattgattta
     2281 tgtaggaagt agcaattgca tggctcgcaa attgagagtc tctttttaga tttaattgac
     2341 aagcttcctc ttcataggga taggaattaa atctaaataa catggcctct ttgcgtccct
     2401 ccttttttaa ataagtaaac tgacctccgc ctttaccatt tcccagaaca tcataaataa
     2461 aatctttttc ttcagaaccc tgtccactca tcgaaatggc ataacatata ccgggcccgg
     2521 aaaatttcat gttcggaatc tctgcaccct gcttatgcaa attcaaaagc tgaaggggat
     2581 aaactttctc tgttttttca atattactag cggcaaaatc atggcaagga agagccatgg
     2641 ctggaaccac taaaaatgga agaaagaaac cgaaaagaaa attctgattc atggcccccc
     2701 ccctaaacaa aggttgtatc ccgaaatcaa ttgaaattgt caagcggttt gagggcaacg
     2761 aactgagccg attcaaagag aatctttttt atctaatttc agcttctgcg cctcttgagc
     2821 gtgatggtaa acaccgtgaa cgaccacacc gatcgcaata agaacaacca cactgactcc
     2881 accaaaaaaa ataattaagc tcatgagttg acaacctcgc ggacgatcca agaagcaatg
     2941 ttaagaccaa aggcaccggt gacaaaactc gctgtgcccc aaataacatt gcggtgttca
     3001 cagctatgca ggttgttctg attttgtggg caaacgcatt taaagccttc gccattgtcg
     3061 taatgcagtt caatgggctg aatgggttct tcatctgaaa acacgcactg aagaccgaag
     3121 ggaccctttg ctggccaatc gtgtttttgc cgcaggattt tgcgcaattg ctgagccatc
     3181 ggatcaccgt aggtgtccgc taaatcagcc cttttaattt gcatggggtc caaccgagcc
     3241 gcggctcctg cgctagtaat cacttttaaa ttttgctgac ggcaagtgtt caacaagtga
     3301 gccttggctg tcaggttatc aatcgcgtcc acaatataat caggtttttt tgcaagaatc
     3361 atttctgagt tttccgcatt gtaaaacacc ggaaccactt caacgaccat ttgcggattg
     3421 attttgcgca gcctctcgcc cataacttcg gctttctttt ttcccaccat accttgaatc
     3481 gcatgcagtt ggcgattggc attggtaatg caaacttcat cgaagtccac caaagtgatt
     3541 ttgcccacac ccgagcgagc taacgactcc gccgcccacg aaccgacacc acccaccccg
     3601 ataatcatca catgggattt aaataatttt aacatggcag cgtcaccgat cagacgaccc
     3661 atacgatcga aacgacggtg taaaacatat ttgctctctt caggttggag agggccctga
     3721 ggagttaaac tattatccat cataatgaaa cagcctttta aaattcgtgg tggcgatttc
     3781 taaaatttcc gtaggttgca tttctcgaag ttcgccaatt ttctccgcaa catcccacaa
     3841 agaggccggt tggttcaagc tttgtttgaa cctgggaggc ggctgatcgg gagaatcgct
     3901 ctcaagcagt aaaaattcca agggcaggtc ccgaatgacg tggtgaagct ttttgttttg
     3961 ctcccaaacc acaggtcccc c
//
LOCUS       DATBTE010000265         9967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2084584, whole genome shotgun sequence.
ACCESSION   DATBTE010000265 DATBTE010000000
VERSION     DATBTE010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 9967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9967
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2084584"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="VIG33_09855"
     CDS             <1..1468
                     /locus_tag="VIG33_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall-associated protein wapA"
                     /protein_id="HEY8271179.1"
                     /translation="GWCSDFETSMEINAEGNIKVKECGGGLEVTFSPREVTKKDIDNT
                     INSIITKMKADKKVGQTETYYKNLQTQLFDDDNMRSEYAHQYQIDVAIKEGTKFYANG
                     KEVEHIVFNKTHYTRNLVDGSIQRFNLQGKLTHIYDKNGNFLKFEYDKDQLRDITDNN
                     SRRLTFKYFSNKKVKSISGPNSLMVEYKFANLDDLSWVKNAWLKVYTYEYDDLHNMVK
                     AGWPDKTFVALKYDKKNDWVTSFTDREKCVESYTYDVDQKDPKNHYWSIVKKICGKET
                     VADNKYEFWHKQRTDGQFYLQRVLSSTNGVTTDISYHEVFGKPLSIRRNSEKISYEYY
                     ADGLVKVKATPTVKMVFEYEPQVKKVNQVTSTFYNENGQKTISKNSQFKYDGKGNLNF
                     AQNSDGQKITMTYDNKGRIATITDQAKKVVKIEYEDRFGKPALVTRPGVGTIKVTYKP
                     SGEINKVESKEGPSVAMQVASTFNNLLDVVAPATSEVF"
     gene            1483..1797
                     /locus_tag="VIG33_09860"
     CDS             1483..1797
                     /locus_tag="VIG33_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271180.1"
                     /translation="MKNILLAITSFFATVLILSQVALAEDDENGDPKHTSSATSEEPN
                     SSQASDKTATEAGVPNPGHKCKEGTCYQRVKGGRLGDSTNPKPSSGSKTQDSSKGEEG
                     RK"
     gene            2042..2251
                     /locus_tag="VIG33_09865"
     CDS             2042..2251
                     /locus_tag="VIG33_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271181.1"
                     /translation="MRIHKTILKLILMASIAAAATTAAASSSCNARCGSSRSANTNPP
                     AKKQVIAKSPSKIPTAGSRSRHGKR"
     gene            complement(2311..2544)
                     /locus_tag="VIG33_09870"
     CDS             complement(2311..2544)
                     /locus_tag="VIG33_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271182.1"
                     /translation="MSELMFVLKSFLLTVILVVAMQVKVGNSSLEQHSQWWLQNSSVS
                     IYVQSVAAGGALAIRNLFFSVKSGVMGKVQAGK"
     gene            complement(2541..2924)
                     /locus_tag="VIG33_09875"
     CDS             complement(2541..2924)
                     /locus_tag="VIG33_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8271183.1"
                     /translation="MSFRYIVIDDTAFVSEIIKNLLKNSGANCVGQAWDGEEGYRLFE
                     KTLPDLVLLDMVMPKENGIELARRIREISRDVVIIACSTLDDKTIIENAIDLGCNDYI
                     VKPFTKEGFLEVLKRHFKFELEKIK"
     gene            complement(2996..4087)
                     /locus_tag="VIG33_09880"
     CDS             complement(2996..4087)
                     /locus_tag="VIG33_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271184.1"
                     /translation="MKFVYSVLIFVFALLNSQSLLAQLDSSYELLLGVRAPTTTSPAS
                     QKVIKKKKREPTGEESATSAQGPVQNPVQVPQGLLMPTQLDTKTLPSQVIEEPTFAQQ
                     AKSLFTANPEKILGFYEEHFEESDPRQNKVDISFASSFVTNDSSSNYSYRSYRSVYAG
                     VNLGANVWLTPAIGIGGNFLFSLGADTSGDAITSTPSPARFEFLDIAVKFRQFYGFAQ
                     TSKSVEFDVLYSDFKFNVSTDDVYRAKLKSSGLGLKMTLRLPSSSEVAWLIGGSFYPR
                     LQHVESKAGADISSGNNVENTRLGVQLGSEIKLSRSSQIFYEAALLAEKNLFSGNAKL
                     VDPVTGSAPKNVSITDTFYIFSLGYRWGN"
     gene            4164..5750
                     /locus_tag="VIG33_09885"
     CDS             4164..5750
                     /locus_tag="VIG33_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271185.1"
                     /translation="MKNNFRALLFCQLSFILLFSLQTKAQSSSSFVSATDQDLTIKTV
                     TSVPFTDNINGIYAKPLYKKFQELLSNERLWNYESLPENTSVPLSLDENQTLVKNLLA
                     STKSQGLFSFHITKGSRGLNGRLIFFVGQTGFPLLQEELTDYQRFEITDLEKQLEGMY
                     EKIKARLPYHGMILSRRGQEVTINLGIKQGLKNNDEISVIQFLKVNRHPKLKFLVSTE
                     KEILGKIKVYKTDESLSFAYVTYEREPGTVQANAKLLPLEFVQYNEPVTQNGQVIPGL
                     ENRPDRELSYGNNPKEWLPVQAPQYGRIAMLAGLGNYGISTNLSTGTIDSSTTMAPEI
                     AVKGELWLSSEWNVLYNLRQSVFNVSNSFAGSTPGRLNMSLSSYSVMGAYNFLMSDDF
                     FGPKIQLSGGYSIYDSHSDQSTPLAFTNMNYSGFVVGFLGQFPLTTDFPMDLGAQFNL
                     FVSPGVRESVSSGDSSKNTINQFSFFGIYHLKSRFKIRGELNFEYYSSDFSGLGAGTH
                     TSTNTTQKLTSFMGGIEYLF"
     gene            complement(5820..7478)
                     /gene="groL"
                     /locus_tag="VIG33_09890"
     CDS             complement(5820..7478)
                     /gene="groL"
                     /locus_tag="VIG33_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162718.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY8271186.1"
                     /translation="MSKELRFSEDARAHILKGVNTLANAVKVTLGPKGRNVVIEKSFG
                     SPLITKDGVTVAKEIELENKFENMGAQMVKEVASKTNEEAGDGTTTATVLAQAIYREG
                     AKIVSAGHNPMSIKRGIDKAVNLIIDDLKAMSKPVKGSNEVAQVGAISANNDKEIGTM
                     LADAMDKVGREGVITIEESKTAKTEVTVVEGMQFDRGYLSPYFVTNAERMETVLENAL
                     VLVYDKKISSMKDMIGILEGVAKQGRQLLIIAEDVDGEALATLVVNKLRGTLQVCAVK
                     APGFGDRRKAMLEDIAILTGAKMISEDMGARLEQATAADLGSAKRIVVDKDNTTIIDG
                     AGKKTEITSRVAQVKAQIEETTSDYDKEKLKERLAKLAGGVAVIHVGAPSEFEMKEKK
                     HRVEDALNATRAAVEEGIVAGGGTALLRASNKIDKSKFSEEEQFGAMIIKRACEEPVR
                     QIAANAGLDGAIVLDRILQNKSNSWGFNAYSDEYTDLIKDGVIDPVKVVRCALVNAAS
                     VASLMLTTETMIAEAPKKEAGMPAGGHGGGMGGMGGMGGMGDMM"
     gene            complement(7518..7805)
                     /gene="groES"
                     /locus_tag="VIG33_09895"
     CDS             complement(7518..7805)
                     /gene="groES"
                     /locus_tag="VIG33_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY8271187.1"
                     /translation="MGVRPLHDRILVRRMAEEEKTAGGLFIPDTAKEKPQKGEIVAAG
                     KGRVTEDGKILPLEVKAGDKVLFSKYAGTELKLDGQEYLMMKEEDILGIFH"
     gene            complement(7950..8510)
                     /locus_tag="VIG33_09900"
     CDS             complement(7950..8510)
                     /locus_tag="VIG33_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271188.1"
                     /translation="MKIKDQLLLVAFTLQIVASIEAGAVTTRGSVRSQALLGASAVVA
                     IPPESPKVKSFREWKSEKVQNALARVTAAKTKIQVVKSKDPNLDRNKANIEANSGANT
                     ETLETQLQQDQSTLEMAKDLSVTDYFAGYLTKLQDKKSAFNEVAGKLTAEEVAELMAA
                     YANSVFGTHSPDLPPSANDISSDRIR"
     gene            8840..9307
                     /locus_tag="VIG33_09905"
     CDS             8840..9307
                     /locus_tag="VIG33_09905"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEY8271189.1"
                     /translation="MNKDFMNKAIQLSLENMRNGTGGPFGAVIVKDGKIIGTGGNKVT
                     STNDPTAHAEIVAIREACGTINNFNLSNSEIYTSCEPCPMCLSAIYWARINKIYYANT
                     RHDAANINFDDDFLYQEIPKDIKDRQVPMIQCCHNDAIEVFKEWQRKQDKIEY"
     gene            complement(9324..>9967)
                     /locus_tag="VIG33_09910"
     CDS             complement(9324..>9967)
                     /locus_tag="VIG33_09910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8271190.1"
                     /translation="NGIIGFSDFLREASIPEEQREYVNIIKQCSHTLLKLINDLLDFS
                     KIDSGCLEIDPHLFKIQDIRNYFENVFLLECKKKKIDFVFEVAEEVPFELFGDSYRIR
                     QVLSNLLSNAIKFTEVGSVKVRVTKLPSEKDIYRWDVIDTGIGIRKDYLPKIFSPYTQ
                     ECSATARKYGGSGLGLSISKKLVELMSGQISVESTLRKGTVFSFTIPLMRLKP"
BASE COUNT         3012 a         2234 c         1967 g         2754 t
ORIGIN      
        1 cggttggtgc tcggatttcg agacttccat ggaaatcaac gctgagggaa atatcaaagt
       61 taaggaatgc gggggcggtc ttgaagtgac tttctctccg cgcgaagtca ctaaaaaaga
      121 tatcgataat acgatcaatt ccattattac gaaaatgaag gcggacaaga aggttggcca
      181 aacagaaacc tactacaaga acctgcaaac acagcttttt gacgatgaca acatgagatc
      241 agaatatgct catcaatatc aaatagatgt ggctatcaag gaaggaacca agttttacgc
      301 caacggcaaa gaagtagaac atatcgtgtt taataagacc cactataccc gtaatcttgt
      361 ggatggaagt attcagcgct tcaatcttca aggaaagttg actcatattt acgataaaaa
      421 cggaaacttc ttgaaattcg agtacgacaa agaccaactg cgtgacatca cggataacaa
      481 tagccgccga ctgactttca aatatttttc caacaaaaaa gtaaaatcaa tctcagggcc
      541 taacagtctg atggttgaat ataagtttgc aaatctagat gatttgtctt gggttaaaaa
      601 tgcgtggtta aaggtttaca cttacgagta cgatgacctt cacaatatgg tgaaggcagg
      661 atggccggat aaaaccttcg tggccctgaa atatgataag aaaaatgact gggttacaag
      721 ctttaccgat cgcgaaaaat gcgtggagtc ttacacctac gacgttgatc aaaaggatcc
      781 aaaaaaccac tattggtcca ttgtgaaaaa aatatgtggc aaagaaaccg tggcagataa
      841 taaatatgag ttttggcaca aacagcgcac cgacggtcag ttttaccttc aacgtgttct
      901 ttccagtacc aacggagtca ccaccgatat tagctaccat gaggtttttg gaaaacccct
      961 ctcaattcgt cgcaacagcg aaaaaatatc ctatgaatat tatgcagatg gactggtgaa
     1021 ggtaaaagcg accccaactg tcaaaatggt tttcgaatat gagccgcaag ttaagaaggt
     1081 caaccaagta acgagcactt tctataatga aaatggtcaa aaaacgatat caaaaaactc
     1141 acagtttaaa tatgacggaa aaggcaattt aaacttcgcc caaaatagtg atggccaaaa
     1201 gataacgatg acctacgaca acaagggccg catcgccacc atcacggacc aagctaaaaa
     1261 ggttgtcaaa atcgaatatg aagaccgctt cggaaaaccc gccctcgtga cgcgtccagg
     1321 agtgggtact attaaagtga cctacaaacc tagcggtgaa attaacaaag tcgaaagtaa
     1381 agaaggccct agtgtcgcca tgcaggtggc tagtactttt aacaatctct tagacgtggt
     1441 tgctcctgca acctcggaag tgttctagga aaggacgaaa atatgaagaa tatacttcta
     1501 gcgatcacca gcttttttgc aacagtgttg atcctttctc aagttgcttt agccgaagat
     1561 gatgagaatg gggaccccaa acacactagt tcagcgacaa gtgaggagcc gaattcaagc
     1621 caggcttcag acaaaacagc tactgaagca ggagtaccga atcccggaca taaatgtaag
     1681 gagggtacat gctaccaaag ggttaaaggt ggccgattgg gtgatagtac caatccaaaa
     1741 cctagcagtg gttcaaaaac tcaagacagc agcaaaggcg aagaaggccg taagtaatca
     1801 tactgcctca taatgaagcc tcatattagc ctcatattga gacggtaagc tcaatttcgg
     1861 tctgagtgtg tctaactaca gaacacctcg accgaaatcc cgattcttaa gttactaaaa
     1921 tccttcactt tttcctcctc tctccagtcg atatttttgg cacagccttc gctatagatt
     1981 cttatgaatg cttagtgcga gctagtgcct aacaaagagt tctcttacca aggaggagac
     2041 aatgcgtatc cataaaacga ttttgaagct tatactgatg gcgtctattg ccgcggcggc
     2101 aacaacagca gccgcaagca gcagttgcaa cgcaaggtgt ggatcttcgc gaagtgccaa
     2161 caccaatccc ccagcaaaaa aacaggtaat tgctaagagc ccttcgaaga ttccgaccgc
     2221 aggttcaaga agtcgtcacg gtaaacgtta gaagggtcca ttattatgaa ggcgaaagat
     2281 cactggtttc tctcccgaac tagtggtctt ttattttccc gcctgtactt ttcccatcac
     2341 gccgcttttt acgctgaaaa ataaatttct aatagctaaa gctccacccg cggcaacact
     2401 ttgaacgtaa attgaaacag aagaattttg caaccaccac tgagagtgtt gctccaagga
     2461 agaatttccc actttgactt gcatagcgac caccaagatc accgtcaaaa gaaagctttt
     2521 tagaacaaac atcaactcgc tcattttatt ttctccagct cgaatttaaa atgtctcttt
     2581 agcacttcta aaaaaccctc tttcgtaaag ggcttcacga tataatcatt acaacctaaa
     2641 tcaatggcgt tctcgataat ggttttatca tcgagagtgg agcatgcaat gatcacgaca
     2701 tcacgagaaa tctcacgaat tctgcgtgcc agctcgatgc cattttcctt cggcatgacc
     2761 atgtccagca gcacaagatc tggaagagtt ttttcaaaaa gacgatatcc ctcttcgcca
     2821 tcccaggctt ggccaacaca atttgccccg gagttcttaa gaaggttctt aataatctca
     2881 gagacgaaag cagtgtcatc aataacaata tagcgaaaac tcatacctta attatcgaaa
     2941 gttctcaggg aattgtctaa atgcttttgt gcaagactag actaaatagg agtttttaat
     3001 ttccccagcg gtagccgaga gaaaaaatat aaaaagtatc cgtaatgctg acatttttgg
     3061 gagctgaacc tgtcaccgga tccaccaact tcgcattgcc cgagaagaga tttttttccg
     3121 ctagcagagc ggcttcgtaa aaaatttgag aagaccttga taactttatt tcagatccta
     3181 actgaacgcc cagacgcgta ttctccacgt tatttcctga actgatatcg gcgcccgctt
     3241 tagactccac atgctgcaag cgcggataga agctgccacc aataagccat gctacctccg
     3301 aactggaggg caatcttaaa gtcattttaa gccctaggcc tgagcttttc aattttgccc
     3361 ggtagacgtc atctgtactc acattgaact tgaaatcgct ataaagaaca tcgaactcga
     3421 cagattttga agtctgcgca aaaccataga actgtctaaa ttttactgca atatccaaaa
     3481 actcaaatcg agctggagac ggtgttgaag ttattgcatc tccactggtg tcagcaccca
     3541 aagaaaataa aaagttcccc cctattccga tggcgggggt caaccagacg ttggccccca
     3601 gattaacccc tgcataaaca ctgcgataac ttctatagga ataatttgag gaagaatcat
     3661 tagttacaaa actagaagca aaagagatat cgaccttatt ttgtcgcgga tcagattcct
     3721 caaaatgctc ttcataaaat ccgagaattt tctccgggtt cgccgtaaaa agacttttgg
     3781 cctgttgagc aaaagtgggt tcttcgatga cttgcgaagg cagagttttg gtatccaatt
     3841 gggtaggcat cagcaaacct tggggaactt gaactggatt ctggactgga ccttgagcac
     3901 tggtcgccga ctcttcccct gtaggctcgc gttttttttt ctttattacc ttttgggatg
     3961 ctggagaagt cgtagtcgga gcccgcactc ccaatagaag ctcataagaa gaatctaatt
     4021 gagccagcaa agactgcgag ttaagaagtg caaatacaaa tataagtact gaatatacaa
     4081 acttcatacc agattttcgg attctctgac gatttacttg atgtgaaatt gacgagtttt
     4141 ttaggtacag gcagaattaa aatatgaaaa ataattttag agccctgctt ttttgccagc
     4201 tctcatttat cctccttttc tctttacaga ctaaggccca gtctagctct tcctttgtca
     4261 gtgccacgga tcaagacctc acgattaaaa cagtcacctc tgttccgttt actgataata
     4321 ttaatggcat ttatgccaag cctctctata aaaaatttca ggaacttctg tctaatgaga
     4381 gactgtggaa ctatgaatct ctgcccgaaa atacttcagt tcccttgagt cttgacgaaa
     4441 atcaaaccct tgtaaagaat ctgttggcct caacaaaatc tcaaggactc ttctcatttc
     4501 atatcaccaa aggatctcga gggctcaatg gccgcctcat atttttcgta gggcagaccg
     4561 gttttccttt actgcaagaa gaactgacgg actatcaaag gtttgaaatt acggatcttg
     4621 aaaagcagct cgaaggaatg tatgaaaaaa ttaaagcccg tcttccctat catggcatga
     4681 ttttgagtcg ccgtggccaa gaagtcacca tcaacttggg tattaaacaa ggtctgaaaa
     4741 ataacgatga aatctccgtc attcaatttc taaaagtgaa ccgccatcca aagctaaaat
     4801 ttttagtaag tacggagaaa gaaattcttg gcaaaattaa agtttataaa acagatgaat
     4861 cactgagctt cgcttatgtc acttatgaac gagagccagg aacggttcag gctaatgcaa
     4921 agctacttcc gctagaattt gttcaatata atgaacccgt cacccagaat ggccaagtga
     4981 tcccaggtct tgaaaatcgt ccagaccggg aactcagtta cggtaacaac ccaaaagagt
     5041 ggctccctgt ccaagctcct caatatggcc gcatcgccat gttagcaggc ctgggaaatt
     5101 atggtatttc cacaaatctt tcgacaggca caatagattc ctcaacgacc atggcccctg
     5161 aaatcgcagt gaaaggtgag ctttggctaa gcagtgaatg gaatgttctc tataatcttc
     5221 gccaatctgt ttttaatgtt tccaattctt ttgctggatc aacaccggga cgcctcaata
     5281 tgtccctgtc ttcttatagc gtgatgggtg cctacaattt ccttatgagt gatgatttct
     5341 ttgggccaaa aattcaactt agtggcggct attcaatata tgattcgcac tccgatcaga
     5401 gtactcctct agcattcacc aatatgaatt acagcggctt tgtcgtggga tttctcggcc
     5461 agttcccatt gacaactgat ttcccaatgg atctcggggc tcagtttaat ttatttgtca
     5521 gtccgggagt cagggagtcc gtgagctcgg gagattcttc gaaaaatact atcaatcaat
     5581 ttagcttttt cggaatttat catctaaaaa gtcgctttaa aattcgtgga gaactcaatt
     5641 ttgagtacta ctccagcgat ttttcaggcc taggcgccgg aacccacacc tctacaaata
     5701 cgacacaaaa gctaaccagt ttcatgggtg gaattgaata ccttttttaa aaataataaa
     5761 cccacggctt tgagaccgtg ggtttataag atatttcagc ttgtataagc cgaacgatat
     5821 tacatcatat cgcccatgcc acccatgcca cccataccgc ccatgccacc accgtgaccg
     5881 ccggcaggca ttccagcctc tttcttagga gcttctgcaa tcatagtctc agtagttaac
     5941 atcaaagatg ctactgaagc tgcattcaca agagcacaac gaacaacttt tactggatca
     6001 ataacaccgt ctttaatcaa atcagtgtat tcatcagagt aagcgttgaa accccaagag
     6061 ttagatttat tttgaaggat acgatccaaa acaatcgctc catcaagacc cgcatttgca
     6121 gcgatttgac gaacaggttc ttcacaagca cgcttgatga tcatcgcgcc aaattgctct
     6181 tcttcagaga acttagattt gtcgatcttg ttggaagctc ttaaaagagc agttccaccg
     6241 ccggctacga tgccttcttc aaccgctgcg cgagttgcat tcaacgcatc ctctacacga
     6301 tgttttttct ctttcatttc gaattcagaa ggagcaccaa cgtggataac agctacgccg
     6361 ccagccaatt tcgccaaacg ctctttcaat ttctctttat catagtcaga agttgtctct
     6421 tcgatttgag ctttaacttg ggcaacacgt gaagtgattt ccgttttttt accagcacca
     6481 tcaatgatgg ttgtgttgtc tttatcaaca acgatacgtt ttgcactacc aagatcagca
     6541 gcagttgcct gctcaagtct agcgcccata tcttcagaaa tcattttcgc accagtcaaa
     6601 atagcgatgt cttcaagcat agctttacga cgatcaccaa aaccaggagc tttcaccgcg
     6661 cacacttgaa gagtgccgcg caatttatta accactaaag ttgccaaagc ttcaccgtca
     6721 acatcttcag caatgatcaa caattgacga ccttgcttag caacgccttc aaggattccg
     6781 atcatgtcct tcattgaaga gatttttttg tcgtaaacaa gaactaaagc attctcaaga
     6841 actgtctcca ttctctcggc attagttacg aagtaaggag aaagataacc acggtcgaat
     6901 tgcatacctt ccactacagt cacttctgtt ttagcggttt tggattcttc gatagtgata
     6961 acgccttcac ggcccacttt atccattgca tctgccaaca tggttccgat ttctttatcg
     7021 ttgtttgcag agatcgcccc cacttgagca acttcgttag agccttttac tggcttagac
     7081 attgctttca agtcatcgat gataaggttc acggctttat cgataccgcg tttgatagac
     7141 attggattgt gacccgcaga aacaattttg gcaccttcgc gatagatcgc ttgagccaaa
     7201 acagttgcag ttgtggttcc gtcaccagct tcttcgttgg ttttagaagc gacttcttta
     7261 accatctgag cgcccatatt ttcgaattta ttttccaatt cgatttcttt tgcaacagtc
     7321 acgccgtctt tagtgataag tggggagcca aaagattttt cgataacaac attacgacct
     7381 ttaggcccaa gggtcacctt tacagcattt gcaagagtgt taacaccttt tagaatgtgt
     7441 gcacgggcat cttcagaaaa acgtaattct ttactcattt tataattctc cttaagtgaa
     7501 tttcttagaa ataaaagtta gtggaagata ccgaggatgt cttcctcttt catcatcaag
     7561 tattcttggc catcgagttt caactcagtg ccagcgtact tgctgaataa aactttatcg
     7621 ccagccttca cttcaagcgg aagaattttt ccgtcttcag tgacacggcc ttttccggca
     7681 gcgacgattt cacctttttg aggtttttct ttagcagtgt ctggaatgaa aagaccacca
     7741 gcggtttttt cttcttcagc catgcgacga accaaaatgc gatcatgaag agggcgaacg
     7801 ccaatctctt tagccatgta aatacctccg aattaatatt taatattagg attagttcga
     7861 agctaatatg aatcgcctga actataagtc aaggcggtga gcggaaaaaa tgtattagta
     7921 cgatgcgtaa atttaattat ttcaattatt tatcttatcc gatccgagct tatatcgttg
     7981 gcactcgggg gaagatctgg agaatgggtt ccgaaaacac tatttgcata tgcagccatc
     8041 aactctgcta cttcctccgc tgttaatttg cccgccactt cattaaaagc agacttttta
     8101 tcttggagct tcgtcaaata gcccgcaaag tagtcagtaa cggaaagatc tttggccatt
     8161 tcgagcgtgg actgatcttg ctggagttgg gtttcgagag tctctgtatt ggctcctgag
     8221 ttggcctcaa tattcgcttt attgcggtcc aggtttgggt ctttagattt aaccacttga
     8281 attttggttt ttgccgctgt tacccgcgcc agcgcattct gaaccttctc acttttccac
     8341 tcacggaagc ttttaacttt aggggattca ggcggtatag caactacagc ggaagctccc
     8401 aagagagcct ggctcctcac agatccacgc gtggtgacgg cgccagcctc aatagaggct
     8461 acaatttgca acgtaaacgc caccaacaac agttgatctt taattttcat gaaaagcctc
     8521 cgcaaaatac gtacaagtaa ttgccaagtc atcttcaagg tccgtgccct ccttttaacc
     8581 ctaccaaatc aaagcaaaac gatttgagac caaatgagaa caattctggg ccctcagagg
     8641 ctaaaaattg actctatttg agatttttca tgatgtacta ttatatataa gccaataaaa
     8701 tcctggattg agatgaggtt ttaccttaaa agttggaagt tttcatcaaa caaggagtct
     8761 atgagtttaa atcattcata ttctaaatga agacaatatt gtagatccat gcgagaaaac
     8821 tcattgttag gaggaagctg tgaacaaaga tttcatgaat aaagctatcc agctgtcttt
     8881 agaaaatatg cgcaatggaa ccggtgggcc atttggcgct gtcatcgtaa aagatggcaa
     8941 aattattggc acagggggga ataaagtgac ctctacaaat gaccccacag cacacgcaga
     9001 aattgtggcc attcgcgaag cctgtggtac tattaataat ttcaatcttt caaattctga
     9061 aatctatacc agttgcgaac cttgtcccat gtgtttgtca gccatttatt gggcccgaat
     9121 caataaaatc tattatgcaa acactcgcca tgacgctgcc aatatcaatt ttgacgacga
     9181 ttttctatac caagaaatac caaaagatat taaagatcgc caggtaccaa tgattcaatg
     9241 ttgtcataat gacgctatag aagtctttaa agagtggcaa agaaaacagg ataagattga
     9301 gtattaaacc ttcagcggaa gcattagggc ttcaatcgca tcagcggaat agtaaaggag
     9361 aagactgttc ctttccgaag ggtgctttcc acgctaattt ggccactcat caattcaaca
     9421 agttttttgg atatggagag accaaggcca ctccccccat attttcgcgc agtcgctgaa
     9481 cattcttgag tgtaggggct aaatattttt ggaagatagt cttttctaat tcctatgccg
     9541 gtatctataa catcccaacg gtaaatatct ttttcggaag ggagtttagt gacccgaacc
     9601 ttaacactac caacttcagt gaatttaatt gcattactta aaagattaga aagaacctga
     9661 cgaattcgat aggaatcccc gaacaattca aagggcacct cttccgcaac ttcaaaaaca
     9721 aagtctattt tctttttttt acactccaac agaaaaacat tttcaaaata attacgaata
     9781 tcctgaatct taaatagatg cgggtcaatt tccagacatc cactatcaat tttactaaag
     9841 tccaaaagat cattgataag tttcaataag gtgtgtgagc actgcttaat gatattcacg
     9901 tattctcttt gttcctctgg gattgaggct tctctcagga aatccgaaaa tcctataata
     9961 ccattta
//
LOCUS       DATBTE010000266         6779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_377164, whole genome shotgun sequence.
ACCESSION   DATBTE010000266 DATBTE010000000
VERSION     DATBTE010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6779
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377164"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            391..2163
                     /locus_tag="VIG33_09915"
     CDS             391..2163
                     /locus_tag="VIG33_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271191.1"
                     /translation="MKTTKHPKRRLQFVLLLFLQIIFLSELRADFSPVRSPEAIHFHH
                     LPVEAIQDPLAKSLFGLCPSVISQQTSECFNPILDSGHPVLQHARTWIAYLDKVLRTR
                     FPDEMVNVPTPYVVLSPSSDFNAAALGTERCYQIAVRPGFESKSVSSPSREEMIEGAS
                     WEIVAPGMIAFDKKLEAIRCESMTLPGKEQELRRELEYFNSGSKNGCQLQLENGELFY
                     NEKCLTNASLTNAFESSVFRVSVSGDAFKINLGAYANSVTEESFIAVLTHELGHYYRQ
                     HADALLPLIAYYYSGRDARQKGRPVPRSEFNTLASRFFKYETYPVSVPPFPGQTLHPI
                     LYSVFQLAEPGNCRKGDQKCQKLCDKSWKFLARDIFENEGASYPQSLDKPSSAFSNAV
                     LKAEKMVLNCAQEIAMSDEVLRSINLQMNTLDAYWYTENNLQKIKANTLAEWYFQISS
                     SLRKLDEDWTKLFSQSLEIQLGFYTEEQEADEFAAHLLTFAGLSPHLEAQIYLDQFDF
                     ASSRNRLELNSLACLAAKNHQWQSWVPIGSMTEHHHSPCFRAYNIEQLIHRENLIQAT
                     GMHPPRVSKEKWAALREIARTQGK"
     gene            complement(2164..2532)
                     /locus_tag="VIG33_09920"
     CDS             complement(2164..2532)
                     /locus_tag="VIG33_09920"
                     /inference="COORDINATES: protein motif:HMM:NF014346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030077 - plasma membrane
                     light-harvesting complex [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="photosynthetic reaction center cytochrome c
                     subunit family protein"
                     /protein_id="HEY8271192.1"
                     /translation="MRNNAIYYKSLIVFGAAIFIIMLILSPKAYTESASKMKTNEDKV
                     RQEMIVISRQLGITCSECHNIQNFASNEKKTFKVAKEHLRIVETLRSNGFDGKKGPEA
                     TCYMCHQGDLHPEYKESLEK"
     gene            2681..4015
                     /locus_tag="VIG33_09925"
     CDS             2681..4015
                     /locus_tag="VIG33_09925"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEY8271193.1"
                     /translation="MKIKHLEDLNTLVSLSKRRGFVFPSSEIYGGLASCWDYGPLGSL
                     LKMNVKRAWWNAMTRRSDIVGLDAAILMHPMVWKASGHVDGFSDPLVDCKECKTRFRA
                     DNTDAYINEKKCPNCSSKNLSEERSFNLMFKTNIGPLEDSSSVIYLRPETAQGHFVNF
                     LNCQQSSRYKIPFGIAAIGKSFRNEITPGNFIFRTREFEQMEMQYFVKPGTDEPFFLQ
                     WKEIRKNFYLKYGIKEENLKFKDHEKLAHYAKAAVDVEFKFPMGFSELEGIHNRSDFD
                     LTQHMKFSGKSLEYFDEANKEKYVPYVIETAVGCDRLILAFMCDAYREEITSDEKGED
                     TRVVMGFHPDIAPIKVAILPLSKKEELSSIAGKLRDQLAEDFDVTYDETASIGKRYRR
                     QDEIGTPLCVTVDFDTINDQAVTVRHRDTMVQERIPMSQLNAYVAGKLKNFS"
     gene            4068..>6779
                     /gene="ppdK"
                     /locus_tag="VIG33_09930"
     CDS             4068..>6779
                     /gene="ppdK"
                     /locus_tag="VIG33_09930"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEY8271194.1"
                     /translation="MHTQTTTSSTGTKTILPQKFVYFFAAGDSEGNAGMKNILGGKGA
                     NLAEMTSLGIPVPPGFTISTEICAHFYDAGGCLPEWVRPAVLEAMKKVEAKIGKKFGD
                     VSDPLLVSVRSGARASMPGMMDTILNLGLNDQTVEGLAKSSNNPRFAWDSYRRFIQMY
                     SDVVMGMNSSLLEVTLEDLKDEKNYKFDTDFKVEDLKHLVKKFKDLIHQMTGSTFPND
                     PYEQLWGAITAVFHSWNTPRAITYRDLHSIPAAWGTAVNIQSMVFGNMGADSATGVAF
                     TRDPSTGEKAFYGEFLIDAQGEDVVAGIRTPQPITKMAAAKTGVKSLEEAMPAAYSDL
                     VTIYKKLETHYRDMQDIEFTIEKNKLWMLQTRNGKRTAKAALKIACDMIDEKLITEEE
                     ALLRLDPASLDQLLHPTLDPKATRTLLAKGLPASPGGVHGEIVFTPEDAVEWKDLGKK
                     VILVRVETSPEDIAGMIAAQGIFTTRGGMTSHAAVVARGMGKCCVAGCGEVEVDYAQQ
                     TMKVKGYVLKKGDVITLDGSTGEVFLGEVKTIEPKLDADFERIMEIADKCRRLKVRTN
                     ADTPKDAQTARKFGAEGIGLCRTEHMFFGADRIDAVREMILAENKQERERALAKLLPM
                     QRSDFHELFKIMEDLPVTIRLLDPPLHEFLPHTEKEIQELAKRINCDVERLHSKIKSL
                     HEFNPMLGHRGCRLAITFPEIYQMQVRAIAEAASQVVKSGKKLIPEIMIPLIATDKEL
                     EILRELTIAEVEKVKKEQHVKFDYQVGTMIELPRAAVTADAIADYADFFSFGTNDLTQ
                     TALGLSRDDAGKFLGSYVSNSIFAKDPFVTIDKMGVGSLVKTATDLGRRSKPNLKVGV
                     CGEHGGDPDSIEFFNSVGLDYVSCSPFRVPIARLAAARAALLAKKLHS"
BASE COUNT         1819 a         1426 c         1642 g         1892 t
ORIGIN      
        1 agaggaaaag aaatgtctgg tttgcataag tcgcgaagga ttttggaata cgaagagaaa
       61 atgggggcgc gactttactt ctgattgcgt acaatcagaa gtccggtaaa agtatgggag
      121 aaagtatcgg gtaaaaaaat agttatagtg attatgaaag caatttaagg agcttttgcc
      181 ttgcaatgga tgtctggcct caagtctgct taaaccctca tccccacttt ttccaaaaga
      241 ggcaattcaa tgtagaactt tttcccatct gcctgccttg ctgcgcactg cttaggtatc
      301 gtctcaattt gattcggttc ttggcaactt aaggcggctt agaaggtaca taagttgcat
      361 ttcctctatg tgatcggaga aagcaccatc atgaaaacaa cgaaacatcc caagcgcagg
      421 cttcaatttg ttttgctatt atttcttcag atcatttttc tgagcgaact tagagccgat
      481 ttcagtcctg ttcgttcccc agaggccatt cactttcatc atcttcccgt agaggcgatt
      541 caagaccctt tggctaagtc tttgttcggt ttatgtccta gtgtgattag tcagcagacc
      601 tctgaatgtt ttaatcccat tttggactcc ggccatcctg ttcttcagca tgccagaact
      661 tggatcgcat atcttgataa agtgctgcgc actcggtttc ctgatgaaat ggtgaatgtt
      721 cctactcctt atgtggtttt gagtccttcg agcgacttta atgcggcggc tcttggcaca
      781 gaacgatgtt accaaattgc agttcgaccc ggcttcgaat caaagtcagt gtcttcgcca
      841 agccgagagg aaatgataga aggagcctcc tgggaaattg tagctcccgg aatgattgct
      901 tttgacaaga aacttgaggc gattcgctgc gaatctatga ctctgcctgg aaaggaacaa
      961 gaacttcgtc gagaactcga atatttcaac tcaggaagta agaatggctg ccaattgcag
     1021 ttagaaaatg gcgagctttt ttataacgag aaatgtttga ctaacgcctc actgacgaat
     1081 gcatttgaga gttctgtttt tcgcgtaagt gtaagtggcg atgcctttaa aattaatctc
     1141 ggtgcctacg caaattctgt gaccgaggaa tcgtttattg cagtgctgac ccatgagttg
     1201 gggcattatt atcgtcaaca tgcggatgct cttttgcctc ttattgccta ttattattcg
     1261 ggccgagacg cgcgacagaa gggaagacca gtcccaagga gcgagttcaa tacgctggcc
     1321 tcgcggtttt tcaaatatga aacttatcca gtcagcgtac ccccctttcc tggtcagact
     1381 ttacatccca ttctttattc agtttttcag ttagccgaac ctgggaactg tcgcaagggg
     1441 gatcagaaat gccagaaact ttgtgataaa agttggaagt ttttggctcg agatattttt
     1501 gaaaatgaag gagccagcta cccccaatct ttggataaac cctcttctgc cttcagcaat
     1561 gcagtcttaa aagctgaaaa aatggttttg aactgcgctc aggaaattgc aatgagcgac
     1621 gaggtgctaa ggtctatcaa tttgcagatg aatactttag atgcgtattg gtacacggaa
     1681 aataatttgc aaaaaataaa agccaatact cttgcggaat ggtatttcca gatcagttcg
     1741 tcattaagga aattggatga ggattggacg aagctgttct ctcagtcact ggaaattcaa
     1801 ttgggtttct atacggaaga gcaagaggct gatgaatttg ccgcccatct gcttaccttc
     1861 gcaggtttga gtccccatct tgaggcgcaa atttatttgg atcaattcga ttttgcctca
     1921 agcagaaata gattagaact caattcgctc gcgtgtttgg cagctaaaaa tcaccaatgg
     1981 caaagttggg tgcctattgg ctcaatgacc gagcatcatc acagtccttg tttcagagct
     2041 tacaacattg aacagttgat tcatcgtgaa aacctgattc aggcaactgg aatgcatccc
     2101 cccagagttt ccaaagaaaa atgggcagcc cttcgggaaa tcgcacgaac acaagggaaa
     2161 taattatttt tccaggctct ctttatattc cggatgcaaa tcgccttggt ggcacatata
     2221 acaagtagcc tcggggcctt tctttccatc aaagccattg ctgcgcaaag tttccacaat
     2281 acgtagatgc tccttggcga ctttaaaggt ctttttctca ttcgaagcga agttttgaat
     2341 attgtggcat tcgctgcagg tgattccgag ctggcgtgag atcacgatca tctcttgacg
     2401 aactttgtct tcgtttgttt tcattttact ggcggactcg gtataagctt ttggtgaaag
     2461 aatcagcatg atgataaata ttgcagcccc gaagacaatg agggatttgt aatatattgc
     2521 gttatttctc atattccatc ctagaattct gaatcaaatt tttgagtgaa actctctttc
     2581 tattttcggt aaagctgtct ccccttacaa gtctgatttc ttgactggtc cagggagcga
     2641 tgttacccag ataaaagact tttttaaaga ggggcatttc atgaagatca agcatctgga
     2701 ggacttaaac actctggttt ctctgtccaa acgtcgtggt tttgtttttc ccagcagtga
     2761 gatctacggc ggtcttgcct cttgttggga ctatggtccc ttgggttccc tcttaaaaat
     2821 gaatgtgaag cgggcgtggt ggaatgccat gactcgcaga agtgacatcg ttggcctgga
     2881 tgcggcgatt ttgatgcatc caatggtgtg gaaggcctcc ggtcacgtgg acggtttttc
     2941 ggatcctttg gtggattgca aagagtgtaa aactcgtttt cgcgccgata ataccgatgc
     3001 ttatatcaac gaaaagaaat gtccaaattg cagtagcaaa aatttgtctg aagagcgcag
     3061 ttttaatttg atgtttaaaa ccaatattgg tccgcttgag gattccagca gtgtgattta
     3121 tcttcgtcca gagaccgcac aaggtcattt tgtgaatttt ttaaactgcc aacaaagttc
     3181 tcgttacaaa attcccttcg gcatcgccgc tattggtaag tctttccgga atgaaatcac
     3241 gccagggaat tttattttcc gtacccgtga atttgaacaa atggaaatgc aatactttgt
     3301 gaaacctggc acggatgaac ccttcttcct gcaatggaag gaaattcgta agaacttcta
     3361 tttgaagtac ggtatcaaag aagaaaactt aaaatttaaa gaccacgaga aactggctca
     3421 ttacgccaag gctgcggtgg acgtggagtt caaattcccg atgggcttta gtgagcttga
     3481 ggggattcat aaccgttcag acttcgattt aactcagcac atgaagttct ctggaaaatc
     3541 tttggaatac tttgatgagg cgaataaaga aaaatatgtg ccttatgtga tcgagaccgc
     3601 cgtgggatgt gatcgcctga tcttggcatt tatgtgtgac gcttatcgtg aagaaatcac
     3661 ttctgatgag aagggtgagg acactcgcgt cgttatgggc ttccatccag atattgctcc
     3721 gatcaaagtg gcgattttgc ctctttctaa aaaggaagag ttgtcgagca ttgctggaaa
     3781 acttcgggat caattggcgg aagatttcga tgtgacttat gatgaaaccg cttctattgg
     3841 gaaacgttat cgtcgccagg acgagatcgg gactccgcta tgtgtgactg tggattttga
     3901 taccatcaac gatcaagcgg tgaccgttcg tcatcgtgac accatggtgc aggagcgcat
     3961 tcccatgagt caactcaatg cttatgtcgc ggggaaactg aaaaatttct cttgatagcg
     4021 cagtatctcc tagtgtctta ttgaaggcgc ataaatagga gacatacatg cacacacaaa
     4081 caacgacatc aagtactggg actaaaacta tactcccaca aaagttcgtt tatttttttg
     4141 cagctggtga ttccgaagga aacgccggaa tgaaaaatat tttaggcggt aaaggtgcaa
     4201 accttgctga gatgacttct ctcggcattc ccgtgcctcc aggattcacc atttccactg
     4261 agatctgtgc tcatttctat gatgccggcg gctgtcttcc agagtgggtc cgacctgcgg
     4321 ttctcgaagc gatgaaaaaa gtcgaagcca agatcggcaa aaaattcggt gatgtcagtg
     4381 atccgctttt agtgtctgtg cgctccgggg cccgtgcttc tatgccggga atgatggaca
     4441 ccatcttgaa cttgggtttg aatgatcaga cggtggaggg tttggcgaag tcctcaaaca
     4501 atccacgttt tgcttgggat tcttatcgtc gatttattca aatgtattct gatgtcgtca
     4561 tgggaatgaa ttcatccctt ctcgaagtga ctctcgaaga tttaaaagat gaaaagaatt
     4621 ataaattcga tacagatttt aaagtcgaag atttaaaaca tctggttaaa aaattcaaag
     4681 atctcattca ccaaatgacc ggcagcacct tccccaatga cccttatgag caactttggg
     4741 gcgctatcac tgcggttttc cattcatgga atactccacg agcgatcact taccgcgatc
     4801 ttcacagcat tcctgcggcg tggggaacag cggtcaatat tcaatccatg gtttttggca
     4861 atatgggtgc cgattcggcg acgggagtgg cctttacccg tgatccaagt actggcgaaa
     4921 aagcattcta tggcgagttt ttgatcgatg cgcaaggtga ggatgtggtt gcgggaattc
     4981 gcacacccca gccgatcact aaaatggcgg cggcaaaaac tggagtgaaa tctctggaag
     5041 aagccatgcc tgcggcttat agcgatttag tgacaattta taaaaaactc gaaacgcatt
     5101 atcgagacat gcaggatatt gaattcacca ttgagaaaaa taaactgtgg atgttgcaaa
     5161 cccgcaatgg caagcgcacc gcaaaggcgg cgcttaaaat cgcttgcgat atgatcgatg
     5221 agaagttaat caccgaagaa gaagctcttt taagattaga tccggcttct ttagatcaat
     5281 tattgcatcc cactctcgat ccgaaagcga ctcgcactct gctggcgaaa ggtttgccag
     5341 ccagtccagg gggggtgcat ggagaaattg ttttcactcc ggaagatgcg gttgagtgga
     5401 aagatctggg taaaaaagtc attctcgttc gtgtcgaaac ttctccggaa gatattgcgg
     5461 gaatgatagc ggctcaaggt attttcacca ctcgtggtgg aatgacttcc catgcggcgg
     5521 ttgtggcccg gggaatgggc aaatgttgtg tcgctggttg tggtgaagtc gaagtggact
     5581 atgctcaaca aactatgaag gtgaagggtt atgtcctcaa aaaaggggat gtgatcactt
     5641 tagatggcag tactggtgaa gtcttcttag gggaagtgaa aacgattgaa ccaaaactgg
     5701 atgcggactt tgaacggatt atggagatcg ctgataaatg ccgtcgtttg aaggtgcgca
     5761 ccaatgcgga tactcctaaa gacgcgcaaa cggctcgcaa atttggagct gaggggattg
     5821 gcttatgccg tactgagcat atgttctttg gcgcagaccg catcgacgct gttcgtgaaa
     5881 tgatcctggc tgagaacaaa caagagcgtg agcgtgccct ggcgaaatta ctgccgatgc
     5941 agcgatccga tttccatgaa cttttcaaaa tcatggaaga tttgccggtg acaatccgtt
     6001 tattggatcc tccccttcat gagttcctgc ctcatacgga aaaagaaatt caagagcttg
     6061 caaaacggat caactgcgat gtggaaagac ttcatagtaa aataaaatct ttgcatgaat
     6121 tcaaccctat gttgggccat cgcgggtgtc gtttggcgat caccttccca gaaatttatc
     6181 agatgcaggt ccgcgccatt gccgaagcgg cttcgcaagt tgtgaagtct ggaaagaaat
     6241 taattccaga gatcatgatt ccattgattg caacggataa agagttggaa atccttcgcg
     6301 agctgacgat tgcggaagtc gaaaaagtta aaaaagaaca acatgtgaag tttgattatc
     6361 aagtgggaac catgatcgag cttccgcgcg cagccgtgac cgccgatgcg attgcggact
     6421 acgcggactt ctttagcttc gggaccaatg acctgacgca aacagcgctg ggtctttccc
     6481 gcgatgatgc cggcaaattt ctcggcagct atgtcagcaa tagcattttc gcaaaagatc
     6541 ctttcgtcac aatcgataag atgggggtgg gttctctggt gaaaacagcc acggacctgg
     6601 gtcgtcgctc taaaccaaat ttaaaagtgg gggtttgcgg ggagcatggt ggagatccag
     6661 attctattga gttcttcaac tctgtaggtc ttgattatgt gtcttgttct ccgttccgcg
     6721 tgccgattgc tcggcttgcg gcggctcggg ccgctctctt agccaagaaa ttacactct
//
LOCUS       DATBTE010000267         3075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1332573, whole genome shotgun sequence.
ACCESSION   DATBTE010000267 DATBTE010000000
VERSION     DATBTE010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332573"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VIG33_09935"
     CDS             <1..309
                     /locus_tag="VIG33_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8271195.1"
                     /translation="VLAALQRSRYDVIFMDCQMPEMDGYEATRRIRKLEKPFSTIPII
                     ALTANAFKEDQTKCLESGMNDFVTKPVDSRTLETKLNRYLKHGSETKPSPKASLQGGA
                     "
     gene            complement(306..1136)
                     /gene="murI"
                     /locus_tag="VIG33_09940"
     CDS             complement(306..1136)
                     /gene="murI"
                     /locus_tag="VIG33_09940"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEY8271196.1"
                     /translation="MGLRKAVLMEFDPRPIGVFDSGIGGLTVLKVLAERFPHESFLYL
                     GDTARLPYGSKSPQTIRRYSEQNIRYLLSQGVKAVVIACNSASSQFTESEFEGVSVYN
                     VIHPGSCKALSLTKNKKIGVLGTRATIKSAAYTQELLCLDENVEVFSQACPLFVPLAE
                     EGWDHDPITNLVAYRYVNPLVQTGIDTLILGCTHYPILKESIRKVTGQGIQLVDSGEA
                     IADLMAQDRERSRFKPAPAEVLQNIQILTTDSSEHFTSLARKTLDPVPAHEFRVVDIN
                     "
     gene            complement(1102..2148)
                     /locus_tag="VIG33_09945"
     CDS             complement(1102..2148)
                     /locus_tag="VIG33_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY8271197.1"
                     /translation="MQPLMFNWQDIKNRSRGLTFDDVLIMPKRSDVRSRRDPSLASKL
                     TKNISLETPILSANMDTVTGCEMAIAMHNLGGLGILHRFMSIEEQAEMVRKVKAAGVK
                     VISASIGVGEEAKTRATILVESGVSVITVDIAHGHSIQMIEAMKWLKDTYPQVEIIAG
                     NLAMPDAARDLIEAGADAIKVGIGPGSMCTTRIITGAGVPQLTAIALCAEIADSYGVP
                     VIADGGIKTSGDIVKAFAAGASTVMLGSMLAGTLETPGEMKHGKKQYRGMASKSAQVS
                     WRGGVPEGMAPEGESTMVNIKGRAKDVIIEIAGGIRSGMSYINATSIAEIREKAFFME
                     MSSNGVAESRAHGV"
     gene            complement(2129..2821)
                     /locus_tag="VIG33_09950"
     CDS             complement(2129..2821)
                     /locus_tag="VIG33_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271198.1"
                     /translation="MNFFARNLQIFKRIGLPLFVLTLIYTSLDQLLTINTEEAMRSPH
                     GANAWIWAYGLGSLIIGIIFPILGVLVVIYGAQNSEANERGLWDYVQHHVNQMSIEIL
                     RCWGKTLFWSILFILPGLWKYFEYTMVPFVVAMSKEYEQGKADALEASIRLVRSCLLK
                     ISAIILIFHLFFPSILTVLFDEYRLVWHTPVEALALTFIDVYLFIFSTQLLMDVFESQ
                     NPQEISNAAAHV"
     gene            2954..3045
                     /locus_tag="VIG33_09955"
     tRNA            2954..3045
                     /locus_tag="VIG33_09955"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2988..2990,aa:Leu,seq:taa)
BASE COUNT          861 a          779 c          690 g          745 t
ORIGIN      
        1 gtattggcgg ccctccagcg cagtcgctac gacgtgattt tcatggattg ccaaatgccg
       61 gaaatggatg gatatgaagc aacccgccgc attcgtaaac tggagaaacc cttttcaacg
      121 atccctatta ttgctctgac tgcgaatgct ttcaaagaag atcaaacgaa atgcttggaa
      181 tccggtatga atgactttgt gacgaaaccg gtggattcca gaactctaga gactaaactc
      241 aatcgttatc tcaagcatgg ctccgagaca aagccctcac ctaaggcttc gcttcaaggg
      301 ggggcttaat taatatccac aactcggaat tcgtgggcgg gaacgggatc caaggtcttt
      361 cgggcaagac tggtgaaatg ttctgatgaa tccgtcgtca ggatttgaat gttttgtaat
      421 acttctgctg gagccggctt aaaacgagag cgttctctat cttgcgccat caaatctgca
      481 attgcttccc cggagtcgac cagttgaatt ccttgacctg tgaccttgcg aatggactct
      541 ttaagaatgg gataatgagt gcaacctaaa atcaacgtat caatcccggt ctgaacaaga
      601 ggattcacat agcgataggc cacaagatta gtgatgggat catggtccca gccttcttca
      661 gctaaaggca cgaataacgg acaggcctgg ctgaaaactt cgacattctc atcaaggcaa
      721 agcaactctt gagtgtaagc cgcactttta atagtggcgc gggttccgag cacaccgatt
      781 tttttattct tggtgagact taaagcttta caacttccag gatgaatgac gttatagacg
      841 gagacccctt caaattcaga ctcagtgaat tgcgaagagg ccgagttgca ggcgatcacc
      901 acggctttca ccccttggct taaaagataa cgaatgtttt gctccgagta gcgacgaatg
      961 gtttgcggcg attttgaacc atagggcaaa cgagccgtat cccctagata gagaaaactt
     1021 tcatgcggga atctctccgc gagcaccttc aaaaccgtga gccctccgat tccagaatca
     1081 aaaactccta tgggacgagg gtcaaactcc atgagcacgg ctttccgcaa ccccattgct
     1141 cgacatttcc atgaagaaag ctttttcccg aatttcagcg atgctggtgg cgttgatgta
     1201 actcatgcca ctgcgaatac caccggcgat ttcaatgatc acatccttgg cgcgcccttt
     1261 gatgttaacc atcgtggatt ccccttcagg agccatgcct tcggggactc ctccccgcca
     1321 agaaacctga gcggatttgg aagccattcc acgatattgt ttttttccgt gcttcatttc
     1381 cccgggagtt tccaaagtgc ccgcaagcat acttccaagc atgaccgtac tagcccccgc
     1441 agcgaaagct ttcacgatat caccagaagt tttaattcct ccatcggcga tcaccggaac
     1501 gccatagctg tcggcaatct ccgcacagag agcaatggcg gtcaactggg ggacgccggc
     1561 tccagtgatg atacgagtgg tgcacatgga accgggaccg attcccacct taatggcatc
     1621 cgctccggct tcgatcaaat ccctggccgc gtctggcatc gctaaatttc cagcgataat
     1681 ttccacctgc ggataagtgt cctttaacca cttcatggcc tcgatcattt gaatcgagtg
     1741 cccgtgggca atatccaccg tgatcacgct cactccggac tccacgagaa tcgttgcccg
     1801 agtcttcgct tcttctccga caccgatgga ggctgagatg actttgactc cagccgcttt
     1861 cactttgcgc accatctcag cttgttcttc aatgctcata aagcgatgaa gaataccaag
     1921 gccgcctaaa ttatgcattg ctatggccat ctcacagccg gtcaccgtgt ccatattcgc
     1981 actcaggatc ggagtctcaa gactgatatt tttggtcaat ttggaggcca acgaaggatc
     2041 ccgacgagaa cgcacatcag aacgtttcgg cataataaga acgtcatcaa aagtcaggcc
     2101 acggctacga tttttaatgt cttgccaatt aaacatgagc ggctgcattg gagatctcct
     2161 gcggattttg gctttcaaaa acatccatta acaattgagt cgaaaaaata aaaaggtaga
     2221 catcaataaa ggtcaaagcc aaagcctcca caggcgtgtg ccacacgagg cgatattcat
     2281 caaataaaac tgtcagtatc gacggaaaaa acaaatgaaa gatcagaata attgccgata
     2341 ttttaagcaa acatgaacgc accaagcgaa tcgaggcctc caaagcatcc gccttccctt
     2401 gctcatactc tttcgacatc gccactacaa aaggaaccat ggtgtattca aagtatttcc
     2461 acagtccggg taaaataaat agtatggacc aaaacaaggt cttgccccag cagcgaagga
     2521 tctcaatact catttgattt acatgatgct gaacgtaatc ccagaggcca cgttcattgg
     2581 cttctgaatt ttgggctcca taaatcacca ccagaacgcc caagattgga aaaattattc
     2641 caataatcaa ggaacccagg ccataggccc aaatccaggc atttgcccca tgagggcttc
     2701 gcatagcctc ttccgtattg atggtgagga gctgatcaag gctcgtgtaa atcagggtga
     2761 ggacaaagag tggcagtcct atacgcttaa atatctgaag gttacgggca aagaagttca
     2821 cacctcaata aaatctggac aggcccctag agtcaaaaga aattttctca gggtgtcatg
     2881 agcttaacgc aaaaacaaat agagatgaca agaagctaaa aaaatgattt tcttagcctt
     2941 catttctaaa catgcctcgg tggcgaaact ggtagacgca caggacttaa aatcctggga
     3001 ttcggttaaa cggtcgtgcc ggttcaagtc cggcccgagg caccattatg ttctacgatg
     3061 tcgatacaat cttac
//
LOCUS       DATBTE010000268         3249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_35314, whole genome shotgun sequence.
ACCESSION   DATBTE010000268 DATBTE010000000
VERSION     DATBTE010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35314"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..517)
                     /locus_tag="VIG33_09960"
     CDS             complement(38..517)
                     /locus_tag="VIG33_09960"
                     /inference="COORDINATES: protein motif:HMM:NF025032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEY8271199.1"
                     /translation="MKKIIFTTVLLAASYGFAAQPAPAPQPAPQTPSLEGTWQLTSRS
                     CSSNAQIKDDLKIGQDSITLTNNPDKTFQYQLVMGGCETKVTGSYTAEGMKVTYKSAT
                     SQSCKDANPIAMSDSYPVFFAYVSDKEAVAIMTGDKAQMSCPAGDALIMHFNKQTKQ"
     gene            775..1320
                     /locus_tag="VIG33_09965"
     CDS             775..1320
                     /locus_tag="VIG33_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271200.1"
                     /translation="MTKYLPLRDVAIFGIFIFACGTGLWACGTSKESEGTKAPAPHFR
                     RAEKAVEEKGTLTLKGEGLKLTPVMQGRKINSLTQAEGAEKYLKLQKKFALPIQFNGW
                     MTLDRVEHNYLKCAQAISKEPSFFLEDDHHGSVPIQPGEKIPLSLEKLYVVHVEFENV
                     SACNSVDIQFGVFYGTDEGAN"
     gene            1450..1620
                     /locus_tag="VIG33_09970"
     CDS             1450..1620
                     /locus_tag="VIG33_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271201.1"
                     /translation="MAQEETHKQAHKETYVDLKQQEVDKKVAKEMLPFFIYAAIPVLI
                     IVFIALKWGPSY"
     gene            1635..2849
                     /locus_tag="VIG33_09975"
     CDS             1635..2849
                     /locus_tag="VIG33_09975"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8271202.1"
                     /translation="MQASIWSPFRHSSYRIFWFCAFLSNLGTWVQDVAASWLMTHLSS
                     SPLVISLLSFSTSLPVVLFSIPAGFWADSGDRRRILLIAQVLMLISAFSLGFQAATGS
                     LSEITILLLSFFMGIGVALNGPAYQTVLSELVSAEEQQTAVLVYYMGLNITRVLGPAF
                     GGFVLGFAGASSAFFINAMSFLGLIIFYWHWPIDKKSSKASEKRGWFDGDFYQKINPQ
                     DWKILFSIHNLKLWLEIFVVSFSASALWALYPVRGRLELHLASLQYGSLLGCLGFGAF
                     LSAVFSKHVMNPQRNWQSLSFAYLVFAAGEILLSFADSYSMACFAMVLAGIGWIILAT
                     LMNMSTRQLTSSSHLKATMLGVFLSVFYLGMSLGSVTWGTLAKETTSSRSLLVSSGFL
                     VLSSLYKYRHKK"
     gene            3086..>3249
                     /locus_tag="VIG33_09980"
     CDS             3086..>3249
                     /locus_tag="VIG33_09980"
                     /inference="COORDINATES: protein motif:HMM:NF014839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEY8271203.1"
                     /translation="MSQITIILPDNSTKVFDHEPSALEVAQSIGPRLAKETLGVKLNG
                     SAEVSDLRIPL"
BASE COUNT          815 a          641 c          762 g         1031 t
ORIGIN      
        1 tctttggcgc aagatttcca ttgagatctg ctgacgatta ctgttttgtt tgcttgttga
       61 agtgcataat caaagcgtcg ccggccggac aagacatttg cgctttatct cctgtcatga
      121 ttgccaccgc ttctttatcg ctaacatacg cgaagaagac tgggtaagag tctgacatgg
      181 cgataggatt cgcatcttta cagctttggc ttgtagcgct tttataagtc actttcatgc
      241 cctctgcagt atagcttcca gtgaccttag tttcgcaacc gcccataact aattggtatt
      301 ggaatgtttt atctggattg ttggttaaag taattgagtc ttgaccaatt ttcaaatcat
      361 ctttgatttg agcgttgcta gagcaagaac gggaagtgag ctgccatgtt ccttcaagtg
      421 atggcgtttg gggagctggt tggggagctg gagcgggttg agctgcaaat ccataagatg
      481 ccgcaagtaa aacagttgta aaaataattt ttttcataaa aaacctcctt attatgtgtt
      541 ggaggcttat tttcgtctat gacgcaaaaa aacgtcaaag atgtttcaga gacgaagaga
      601 agaataaaaa gtcatcgttc attgctgatt aaaataatct gatttaggca aggagggtgg
      661 agtggtcctc tctttcttgg ttggagataa agtctttgtg acggagaaag gatgtaactt
      721 catttcagca tagctgcgat atctggcata gtggccgcgt taggaggagg tcttatgaca
      781 aagtatttac ccctcaggga cgttgcgatc ttcggcattt ttatttttgc ctgcggcact
      841 ggtctttggg cttgtgggac atccaaggag tcggaaggaa ccaaggctcc agcacctcat
      901 tttcgcaggg ctgagaaggc cgttgaagag aaaggcactc tgacattgaa aggcgaaggt
      961 ctgaaactga ctccagtcat gcagggacga aagataaaca gtctcacgca ggccgaagga
     1021 gcggaaaaat atttgaagtt gcagaaaaaa tttgcgttgc ccattcagtt taacgggtgg
     1081 atgaccttgg accgtgttga acataattat ttaaagtgtg ctcaggcgat cagtaaagag
     1141 ccaagctttt ttctggaaga tgaccatcat ggttccgttc ccattcaacc tggtgaaaaa
     1201 attcctttaa gtcttgaaaa gctctatgtt gtgcatgtgg aatttgaaaa tgtctctgcc
     1261 tgtaacagtg tcgatattca atttggagtc ttttatggaa ctgatgaggg ggccaactga
     1321 gcgaatcaaa atgaatgaac caatcaataa cttaagcgtg tgatttcttg atcttcgcca
     1381 agctttcgcc tagaatacat ttgaattgct taaaacgacg gcaaaaattt tgataaaaag
     1441 gggcgggtta tggcacagga agaaacacat aaacaagcac ataaagaaac atatgtagat
     1501 ttgaaacaac aagaagtaga caaaaaagtc gcgaaagaaa tgttgccatt ttttatttat
     1561 gcggcaattc cagttctaat tattgtcttt atcgctttga agtggggtcc tagttattag
     1621 gattgaaccc tcaggtgcag gcatcgattt ggtctccttt tcgtcattcc tcatatagaa
     1681 ttttttggtt ttgtgctttt ctttccaatt tgggaacttg ggttcaggat gtggcagcca
     1741 gttggttgat gacgcatctt tcgagctctc ctctggtgat ttctctgctt tctttttcca
     1801 ccagtctgcc tgtggtttta ttttccattc ctgcgggctt ttgggctgat agtggggatc
     1861 gtcgcagaat tcttttgatc gctcaggttt tgatgctgat ttccgcgttc agcttgggtt
     1921 ttcaagccgc gactggttct ctctctgaga tcacgatttt gcttttaagc ttttttatgg
     1981 gtattggcgt ggcgctgaac ggccccgctt accaaacagt gctttctgag ctggtttccg
     2041 cggaagaaca acaaacggcc gttcttgttt attacatggg attaaacatc acccgagttc
     2101 ttggtcccgc ttttggtgga ttcgttttag gattcgccgg agcgagcagt gcttttttca
     2161 taaatgcaat gtctttcttg ggtttaatta ttttttactg gcattggcca atcgataaaa
     2221 aatcctctaa agccagtgaa aaacggggat ggtttgacgg tgatttttat caaaaaatca
     2281 acccccagga ttggaaaatt ttattctcca ttcataatct aaaactctgg ttggaaattt
     2341 ttgtggtgag tttttcggct tcagcgcttt gggctctcta tcccgtgcgt gggcgtttgg
     2401 aattgcactt ggcttcttta caatatggtt ctctgttggg ctgcttggga tttggagctt
     2461 ttttaagtgc cgtgttctca aagcatgtga tgaatccgca gcggaattgg cagtctttaa
     2521 gctttgcata cttggttttt gccgccggcg aaattttatt aagttttgcg gacagctatt
     2581 ccatggcctg cttcgcgatg gtgcttgcgg gaataggctg gattattcta gcaacgctta
     2641 tgaacatgag tactcgtcaa ttaacttcga gctcccattt aaaagccact atgctggggg
     2701 tttttctctc tgttttttat ctaggaatgt ctctgggcag tgtcacatgg ggcaccctgg
     2761 ctaaggagac cacaagctca cgctctctct tggtctcttc gggatttttg gttctcagtt
     2821 ctctttataa atatcgccat aaaaagtaac cagacttagg attcgtagtg gtaattaacg
     2881 cgtagcaacg atagattgag accagattga gggtattaag aaaaagccgt caacgattaa
     2941 tcattgaccc ctaagccccc tcgttttaag tatatcacat gtataatttt cttaagaaat
     3001 tgttagccac ctctactcct tcggtcgcga ctccttcgat tgtaaacatt tcgattgcaa
     3061 gcacagcgac aaccttagtt tcataatgtc acaaattaca attattttgc ctgataattc
     3121 tacgaaggtg tttgatcacg aaccctcggc actcgaggtg gctcaatcta tcggtccacg
     3181 tcttgccaaa gaaactcttg gtgtgaaact caacggttcc gcggaagtct cggatctgcg
     3241 gattcctct
//
LOCUS       DATBTE010000269         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_992124, whole genome shotgun sequence.
ACCESSION   DATBTE010000269 DATBTE010000000
VERSION     DATBTE010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992124"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..521)
                     /locus_tag="VIG33_09985"
     CDS             complement(123..521)
                     /locus_tag="VIG33_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271204.1"
                     /translation="MNKQQNSTIQNGQLAEYLKTARAAARGHVVKSSDIERVVRERLT
                     RAGYLSEVMNGWYLLSTPAGAGTTTLWFSNYWEFIREYLKERFGEEGYCLTPESSLDI
                     YAGQNVISRQVTIITKKASNQTINLEMSHF"
     gene            1091..2077
                     /locus_tag="VIG33_09990"
     CDS             1091..2077
                     /locus_tag="VIG33_09990"
                     /inference="COORDINATES: protein motif:HMM:NF023776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3644 domain-containing protein"
                     /protein_id="HEY8271205.1"
                     /translation="MKSRSRSIFEKSVSAIMASIELYNKPDFFYREECFSILAVNAWE
                     LLLKARILQLSSNSLHSIAEYDNPQTDQGKKSKVRKKLRNRAGNVQTIGIFKAYDLLV
                     SDFSEPMHESIRKNLEALVEIRDNSVHLINKGLDLERKILEIGTATLNNYIKISRQWF
                     GSDLSKYNIFLMPIAFIREIPKAEGIVLNGEERNIANYFSSLNKELRKENESDFNTAL
                     SIEIKLKRSKEDSAVKFSISNDPDAVSVKLEEADIREKYPWSYQLLTEKLKQKHGPLF
                     KTNEKYHKIRKELEKSDKYCMVRLLDPGNAKSSKMRFYNSNILKEIEKKYFE"
     gene            2172..>2718
                     /locus_tag="VIG33_09995"
     CDS             2172..>2718
                     /locus_tag="VIG33_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271206.1"
                     /translation="MGAEYQVIIPDSGTASEIIERIDESKYLCIKRHCQIINEFFEDY
                     LGYWILEGNIDSFYRYTSYDHSKILLKRSLSETEHERVRIQLNRKFLNILSSFRSFID
                     HADTAVARQAAKGDDISKIFKLAQAKEFDSNLYYRFIWDLRNYAQHCTLPINNYGIDI
                     KSSKNKLKFKANLVPFLETAHL"
BASE COUNT          932 a          492 c          500 g          794 t
ORIGIN      
        1 atacgagcat atccaatcag tagtagaaag aatcgaaaaa ttacaaacag aaattattga
       61 caacaaaaac aaaaaggcag catgatctga atcacttcac gaaagtggct ccacactttc
      121 aactaaaaat gagacatctc caaattgatt gtctgattac ttgccttttt agttataata
      181 gttacttgtc gtgaaattac attctgacct gcataaatat ctaatgaaga ctcgggtgtt
      241 aaacaataac cctcctctcc aaaccgttct ttgaggtatt cacgaataaa ctcccaataa
      301 ttactaaacc ataatgtagt tgttccagcc ccagcaggtg tagaaagcaa ataccatccg
      361 ttcattactt ctgaaagata gccggccctt gtaaggcgtt ccctaacaac tctttcaata
      421 tcagaagact taacgacatg gccccgagct gctgctcttg ccgtctttaa atactcagca
      481 agttgaccat tctggatagt agaattttgc tgtttattca cttagacctc aacttttctt
      541 tgtataatca tacgagtaat gcaacatact agaaatttat gctatcaatt actagatatt
      601 catgcaatta attactagat atttatgaag caatttgtta gatgtttatg tgtaaatgtc
      661 aattaaagct ttatctaatt aaaaatcaaa taatattagc tatttatata ctagaaaaca
      721 gtgcagttaa ctggccaaac gcctaaatat tcttgtattt taaagttagt aggtggctga
      781 gaaagcattt tagagttttc tgagggtatt ttttgttccg attacgccga atcccctcag
      841 ggtctttttg accaagggca tttgtggcca ttgccaggat tgtgatcatt tacttgtcac
      901 gaaagtgatt ggctaccgcc ttggaagcat tgctttttat aacaatacta aagtcgtgaa
      961 ttacaattat atggcaacca agcttaatct agaatgacaa gaacttccta aatcgtataa
     1021 acatatattt atctatatca atattgggtc gttgccgaac gcaaaaatac cagaataaga
     1081 ggtcgataat ttgaaatcga gatcaagaag tatcttcgag aagtctgtta gcgccataat
     1141 ggcatcaatt gagctttata ataagcctga ctttttttac agagaggaat gtttttctat
     1201 tctcgcggta aatgcttggg aactactttt aaaagcgaga attttgcagc tatcatcgaa
     1261 ttcattgcac agtattgcag aatacgacaa tcctcagacg gaccaaggaa agaagtcaaa
     1321 agttagaaaa aagttgagga atagagcagg caatgttcag acgattggaa tttttaaagc
     1381 ctacgacctc cttgtttccg acttttctga gcccatgcat gagtccataa ggaaaaattt
     1441 agaggcttta gttgaaatta gagacaactc agtccatttg attaataagg gcttagatct
     1501 cgagagaaaa attctggaga ttggaacagc gactttaaat aactatataa aaataagccg
     1561 tcagtggttt ggaagcgacc tttcaaaata taatattttt ctcatgccca tcgcattcat
     1621 tcgagaaatt ccgaaggcgg aaggcattgt cttaaatggc gaggagcgaa atatagccaa
     1681 ctatttcagt agtctaaata aggagctacg aaaagagaat gagtccgatt tcaacacggc
     1741 tttgagtata gaaattaaat taaagcgttc aaaagaagac tcagctgtta aattttctat
     1801 ttccaatgat cctgatgcgg tatccgttaa gctggaagaa gcggacatac gagaaaaata
     1861 tccgtggagc tatcagctat tgacggaaaa acttaagcag aaacatgggc ctctttttaa
     1921 aacaaatgaa aagtatcata aaatcagaaa agaattagaa aaaagtgaca aatactgcat
     1981 ggtaagactg ttagatccag gcaatgcaaa aagttcgaaa atgcgattct acaattctaa
     2041 cattttaaag gaaattgaaa aaaaatattt tgaatagcga atttcctatt tcgccgacgg
     2101 acattatttt cttcgtcgat atttctgccc gatttcagat gataacaaca taaaaagtgg
     2161 agcgtgtgta cgtgggtgcc gaatatcaag tcattattcc cgattcgggg acagcgagcg
     2221 aaatcattga gagaattgat gaaagcaaat acctttgtat caaacgacac tgccagatta
     2281 tcaatgagtt ctttgaagac tatttaggct attggatttt ggagggtaat attgactctt
     2341 tctaccgata tacatcttac gatcattcta agatcctgct gaagcgaagc cttagcgaaa
     2401 ccgagcacga aagggtacga atccagttaa atagaaaatt tttgaacata ctctcttcct
     2461 ttcgctcatt catcgatcat gcagatacgg ctgtcgcaag acaagccgcc aaaggcgacg
     2521 acatctcaaa aatatttaaa ttggcgcagg ctaaagaatt tgattcaaat ttatattatc
     2581 gatttatttg ggacttgaga aactatgctc agcactgcac acttccaata aacaattatg
     2641 gaattgacat taaatcctcc aagaacaaat tgaaattcaa ggcaaatcta gtgccgtttc
     2701 tagaaacggc acatctgc
//
LOCUS       DATBTE010000270         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1675182, whole genome shotgun sequence.
ACCESSION   DATBTE010000270 DATBTE010000000
VERSION     DATBTE010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675182"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1111
                     /gene="tadA"
                     /locus_tag="VIG33_10000"
     CDS             170..1111
                     /gene="tadA"
                     /locus_tag="VIG33_10000"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY8271207.1"
                     /translation="MKEPLNQQLLDEKWMSHALKLAAKASSLGEVPVAALLIGPDGKT
                     LLAQSYNHRESWQSPLAHAEVIVLHTASKKIHNWRLENCTLYVTLEPCLMCAGALLQS
                     RVKRVVYGVKDPKSGAVDSLYKTFQDLRLNHQIEVTAGILEQNCVKLLQDFFKSRREE
                     HKIVKQQKIYRRRASVIVVHQNKVLGFHAMDPTSKKHYFFMPGGKIEKNESAVEAAIR
                     ETLEETGYKIRILPNHELRRRYDFEWDGRVNHCDTSFFVGILDEDWFEPVPVKDAPYN
                     KGPQWLPVKNLDDIFNYHPDILWGARWGVKKSLLRPD"
     gene            complement(1108..1791)
                     /locus_tag="VIG33_10005"
     CDS             complement(1108..1791)
                     /locus_tag="VIG33_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8271208.1"
                     /translation="MAQLNRKILIVDDYEESCKLLSEILSSTYECYYTSDSTKAFSLV
                     TEKKPDLVLLDYKMPGLMGIDVCKMIRENESTKNIPIIFVSGAATIDEKIRAFEQGAD
                     DFISKPFHVRELILRIKARLSEKDHDLSAELQAANLRMNLLSRQVFVDGEEVQLTPKQ
                     FDILKMLVSNKNNLVTREKCLDEIWGDTEVTSRNVDSQINYLKRKINKFQGKILAVPS
                     LGYRLEVHT"
     gene            complement(1902..2267)
                     /locus_tag="VIG33_10010"
     CDS             complement(1902..2267)
                     /locus_tag="VIG33_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HEY8271209.1"
                     /translation="MSQKNTKLVYSTDPKDQKCLKCKEYLSECTCLKQETVSSRNFIA
                     IFRLEKNARGGKVVTVIDALPKIEVYLKDLCKELKTKCGVGGTYVLSGKEGLIEIQGD
                     KRVQIKKIFDQKGIKYKGA"
     gene            complement(2264..>2554)
                     /locus_tag="VIG33_10015"
     CDS             complement(2264..>2554)
                     /locus_tag="VIG33_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEY8271210.1"
                     /translation="LFHMTNFGQPIIRIEDANFENRGELLMTHLHEGIDMQPDFMTET
                     LKNLYKIWSRPVNLATAMDGEGQLFRFDGKEFKNYKLNQGPRPNSSNTENGN"
BASE COUNT          743 a          555 c          481 g          775 t
ORIGIN      
        1 aatggtaaaa gtgttgaatt gccttcaatt gacactgcct tattaaatca ataaactgta
       61 tttccgcctg ctcaatcata aattattgat attatttaga tttttctttt tctaaatttt
      121 tcttggatgc ttcttgaact ccaaggacag agggcggctt gaggacatta tgaaagaacc
      181 cctaaatcaa caacttcttg atgaaaaatg gatgtcacat gctctgaagc ttgccgctaa
      241 agccagttca ctaggtgagg tcccagtggc agctctcctt attggccctg acggcaagac
      301 cctcctggct caatcctaca atcatcgcga aagttggcaa agccccttag ctcacgccga
      361 agtcatcgtt ctgcatacag cttctaaaaa aatacacaat tggcgattag aaaattgcac
      421 attatacgtg acgcttgaac cttgcctgat gtgtgcggga gcccttttac aatctcgagt
      481 aaaacgcgtg gtttatgggg taaaagatcc gaaaagtggg gcggtggatt ccctttataa
      541 gacttttcaa gaccttcgac ttaaccacca aattgaagtt acggcaggaa tactagaaca
      601 aaactgcgtt aagcttcttc aagatttttt taaatcccgc cgtgaagagc ataagatcgt
      661 aaaacagcaa aaaatctatc gccgccgagc cagtgtgatt gttgtccatc agaataaagt
      721 acttgggttt cacgccatgg acccaacaag taaaaagcat tactttttta tgcctggagg
      781 aaaaattgaa aagaacgagt ccgctgtaga ggcagccatt cgggagactc tagaagaaac
      841 tggctataaa atccgaattt taccgaacca cgagcttcgc cgtcgctacg attttgagtg
      901 ggatgggagg gttaaccact gcgacacaag cttttttgtc ggtatcctgg atgaagattg
      961 gtttgaacct gttcccgtga aagatgcacc ctacaataaa ggaccccagt ggcttccagt
     1021 aaaaaacttg gatgatattt ttaattatca tcctgacatt ctatggggag caagatgggg
     1081 agtaaaaaaa agtcttctaa ggcctgatta ggtgtgaacc tcaaggcgat aaccgagact
     1141 gggaaccgca agaatcttgc cttgaaactt attgatctta cgttttaggt aattaatttg
     1201 tgaatccaca ttacgtgagg ttacttctgt atctccccaa atctcgtcta aacatttctc
     1261 tctagttacg agattattct tgttagatac gagcatttta agaatatcaa attgctttgg
     1321 agtcaattgg acttcctcac cgtctacaaa cacttggcgt gacaaaagat tcattcgcaa
     1381 attcgcagct tgaagctccg cgctcaaatc gtgatctttc tccgaaagcc gcgccttaat
     1441 acgaagaata agttctctta catggaaagg tttggagata aaatcgtctg ccccttgctc
     1501 gaaagcacga attttttcat caatagtcgc cgctccagaa acgaagataa tgggaatatt
     1561 ttttgtcgat tcattctcgc gaatcatttt gcaaacatcg atccccataa gaccgggcat
     1621 tttatagtcc aataaaacaa gatctggttt tttttcagtc actagcgaaa acgccttggt
     1681 actatcggat gtataatagc attcataagt tgaactcaaa atttcagata aaagtttaca
     1741 actttcttcg taatcatcaa ctatcaatat ttttctattt aactgagcca ctcttcttct
     1801 ctccctttgt cgcacatact taggctcgtc gaaagaattt aatgacgcaa ggctagtact
     1861 gcatcattcc acaacaaacc tttttttaca ggtataagct tttacgcacc tttatatttt
     1921 ataccctttt gatcaaaaat cttctttatt tggacgcgtt tgtccccttg tatttcaata
     1981 agaccctctt tacccgacag aacataggtg ccacctacgc cacatttggt tttcaattcc
     2041 ttacagagat ctttaaggta aacttcaatc ttaggcaaag cgtcgatcac agtcacgacc
     2101 ttaccaccgc gagcattctt ttctaagcgg aatattgcga tgaagttcct ggagctaacc
     2161 gtttcttgtt taaggcaagt acactcagag aggtactctt tgcacttcaa acacttttga
     2221 tctttggggt ctgtcgaata aacgagttta gtatttttct ggctcaattt ccattctctg
     2281 tgtttgaaga gttaggacga ggtccttgat ttaatttata gttcttaaat tcttttccat
     2341 caaatctaaa aagttgcccc tctccatcca ttgcagtagc cagattcacg ggacggctcc
     2401 agattttata gagatttttc aaagtttcag tcataaaatc cggctgcata tctatgcctt
     2461 catgcagatg cgtcatcaat agctcaccac gattttcaaa atttgcatcc tcaatacgaa
     2521 taatcggctg gccaaagttt gtcatgtgaa acaa
//
LOCUS       DATBTE010000271         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2222036, whole genome shotgun sequence.
ACCESSION   DATBTE010000271 DATBTE010000000
VERSION     DATBTE010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2222036"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..939
                     /locus_tag="VIG33_10020"
     CDS             175..939
                     /locus_tag="VIG33_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271211.1"
                     /translation="MKYFLKLIDVARENGIVTNPEEWETRPLTESEWDAIEKCAPFVF
                     TDVEETVHVNGIESDLSDAPFPIFSIEGFGTEPAGEYMGPVVGLLPLYCMISIELKPK
                     IFDYIALSKAGDEYFVLQMDERKVGPIVTSFLQDLSKKSLGQERVRTSIKIGTGKMKR
                     LHRLRRIIHVAPRKYARAAPETHRHIDWTHRFEVRGHWRKIDTLGKDRQGKYCIHGYT
                     WISHHEKGPETLPLVRKVRLFDQSKVDADAVTPFKL"
     gene            948..1514
                     /locus_tag="VIG33_10025"
     CDS             948..1514
                     /locus_tag="VIG33_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271212.1"
                     /translation="MSTQKKKTRVTKNWPPIAYIILGIDILAIILLRENNPAQIPAIL
                     LSSVALGFSYYAYRFSKEKFRLDLLNRRWEIYEQVLKFCSVVVIHGGLPKYGEDESIN
                     KSVVSGLEAAHESFRGIGLHKAKSLFGPEVHEKFEQLNKMYAWFVARPRTDGWAETEH
                     QHLLDILKTIENLPTVFKPYIYFGDYKD"
     gene            complement(1933..>2342)
                     /locus_tag="VIG33_10030"
     CDS             complement(1933..>2342)
                     /locus_tag="VIG33_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271213.1"
                     /translation="RSGFHVGVLAYSGSDLVGWVSVGTLPEFYWTWRRVAQVGEGARD
                     IAGIVCFTISSKYQNQGLQKLMLEELKSYGRCRGWKTIEGYPFDQSAIEKHKEHVRWP
                     GLTKGFVDAGFEHVGPHWLSNPEAERSIYRVQL"
BASE COUNT          661 a          503 c          530 g          648 t
ORIGIN      
        1 cgaactttcg acaatttgta atgcttttga aaataaaatc gaaggaaacc tttacgacgg
       61 aaatcaattt ccattgaatc cggtcccttt tgctatggcg ttaggcattg gtccatggga
      121 ccggtaaacc ggtaaaaaaa gacccattta aaaccggtgc ttccaggtac accgatgaag
      181 tactttttaa agctcattga tgttgcacgg gaaaatggca tagtgacaaa tcctgaagaa
      241 tgggaaaccc gccctttaac cgaatccgag tgggatgcta tcgaaaaatg cgctccgttt
      301 gtttttaccg atgtcgaaga aacagtgcat gtaaacggta ttgagtcaga tcttagcgat
      361 gccccatttc ccattttttc catcgaaggt tttggaacag aacctgcggg tgaatatatg
      421 gggccagttg tcggtcttct tccgctatat tgcatgattt cgattgaact taaaccaaag
      481 atttttgatt atatcgctct tagtaaagcg ggtgatgagt attttgtttt gcagatggat
      541 gagcgcaagg ttggccccat agttaccagt tttctgcagg acctttcaaa gaaatctctt
      601 ggacaagaaa gagtccgaac ttccatcaaa attggaaccg ggaaaatgaa gcgacttcat
      661 cggcttcgca gaatcattca tgttgcacca agaaaatatg cccgtgccgc gcccgaaaca
      721 cataggcata ttgattggac gcatagattt gaagtgcgcg gccattggag aaaaatcgat
      781 accctgggaa aagatcgtca aggcaagtac tgtattcacg gttacacatg gatttctcat
      841 cacgaaaaag gcccagaaac tctacctctg gttaggaaag ttcgtctttt tgatcagagc
      901 aaagtcgatg ctgatgccgt aacaccgttc aaattgtgag gttcgcaatg agtacgcaaa
      961 agaagaagac aagagtcaca aaaaattggc ctcctatagc ctacataatt ctcggcatcg
     1021 acattttggc gattatttta ctacgcgaaa ataaccccgc tcagattcca gcaattttac
     1081 ttagttcggt tgcattggga ttcagttatt acgcctatag attttcaaaa gagaaattca
     1141 ggttggattt gctaaataga cgctgggaaa tctatgagca ggttcttaag ttttgcagtg
     1201 tcgtcgtgat tcatggaggg cttccgaagt acggcgaaga tgagagtatt aacaagagtg
     1261 tcgtttctgg attggaggca gcccacgaat cttttagagg cattggcctt cataaagcaa
     1321 aatcactctt tggaccggaa gttcacgaaa agttcgagca attaaataaa atgtatgcat
     1381 ggtttgttgc tcgcccccga actgacggtt gggctgaaac agaacatcaa cacttgctcg
     1441 acattcttaa aactattgag aatctaccca cagtatttaa gccatatata tattttggcg
     1501 attacaaaga ttagtctgta atccaactac cactaattca ctgattgaac gatttgttat
     1561 tggagatttc cctgatggaa tacagcacgt ccggcgaacc ccacctgctg gctttgggta
     1621 gttccaagag cttcacctga gtgtgtcgta cctgatttgt catgtgaggt agtcggccag
     1681 tcagtgcggc ggcagagtca tcgtgagtgc gagtttgatg acataattga gtcccatgtg
     1741 gatgtttcat cggttgtaac ggcgcttgca tctgttcatg gggttaccac agcggaaatg
     1801 ggtaatgcta tttggagtta tctgtcgcgc atcgtatctg aaaactagag gatattattc
     1861 gtcctgaact cgcatttgtc ggtaatttat tgagagggat ggccagtacc tcacttaggg
     1921 ggcgctggct tttcaaagtt gaactcgata aatggatcgt tctgcctcgg gattggataa
     1981 ccaatgaggc cctacatgtt caaatccggc gtcgacaaaa cccttcgtaa gccccggcca
     2041 tcgaacatgt tccttgtgtt tttcaattgc actttgatca aacgggtatc cctcaatggt
     2101 cttccagcca cggcaccgtc catatgactt caattcttca agcatcaatt tttgaagacc
     2161 ttggttttgg tattttgatg aaatagtaaa gcaaactata ccagcaatat cacgggcacc
     2221 ttctcccacc tgcgccactc gtctccaagt ccagtagaat tcaggcagag ttccaacgga
     2281 aacccatccg acaagatcgc ttccactgta agcaagaaca ccgacatgaa aaccagatct
     2341 ca
//
LOCUS       DATBTE010000272         2937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1606732, whole genome shotgun sequence.
ACCESSION   DATBTE010000272 DATBTE010000000
VERSION     DATBTE010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606732"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(355..1143)
                     /locus_tag="VIG33_10035"
     CDS             complement(355..1143)
                     /locus_tag="VIG33_10035"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEY8271214.1"
                     /translation="MLQEQGLSFRRSCGLAGIGRNTANYQPSEKDMPLVARMKELATE
                     HFRYGYRRIWALLRREGWVVNVKRIWRLWKMSKLQVPKRKPKRRRQGQGVPLAPCQAI
                     APNHVWTLDFVADRLSHGSRLRMLTVVDEFTRECLTIRVERSLKAKDVQETLAQVMKE
                     RGHPCYLRSDNGSEFIEKSLQKWLKEKGVAAIFIDPGSPWQNGKCESFNGKLRTECLN
                     ATYFRTLREAKVLIEMWRKEYNIFRPHRALKLHTPTEFAQSWNA"
     gene            complement(1173..1439)
                     /locus_tag="VIG33_10040"
     CDS             complement(1173..1439)
                     /locus_tag="VIG33_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8271215.1"
                     /translation="MKKSRFSEEQIVKALQEHEVGGKTIVAISREMGIAQATFHKWKQ
                     KYGGLNVNEARRLKELETENSRLKRMVADLMLENEAVKDVLKKL"
     gene            complement(1486..2169)
                     /gene="dgt"
                     /locus_tag="VIG33_10045"
     CDS             complement(1486..2169)
                     /gene="dgt"
                     /locus_tag="VIG33_10045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HEY8271216.1"
                     /translation="MDWEKLLCEKRLGRKDEDPTQLEEYPFRTEFEKDRDRIVFSSAF
                     RRLQAKTQVHPTPDTDYTRTRLTHSIEASSVGRSLGNYVGHKICKDDKPFSTKGHSHT
                     DFGDIVYSACLAHDIGNPPFGHSGEKAISDWFKNNKNVLDGLTQPQKDDFLKFEGNAQ
                     GFRILTRLSGPCKRDHSYEPGGLQLTYATLGAFLKYPLTTQVAESVKADDSSAKKFST
                     DVPPIKRAS"
BASE COUNT          726 a          745 c          630 g          836 t
ORIGIN      
        1 gtccttcaac ggcgacaacc ctcctgcctg tgctgattct tcccacagca aagcacaagc
       61 aggggtggta cacttttaca ccgccgtttt tgttaaaaac ggtacacttt tagtatgccg
      121 tttccagtgc tgactggggc cttaaattag atccactttg aattagagat tttgggatca
      181 tagcacgacc tgttctttgg ggcataaaac acctcgccgt aaaaaagtca gaagaaatgg
      241 ctgtaaaaac ctattggaaa gccctcaggc ttttcaatgg gttttctaca gcttacattt
      301 gccttcggca cgctttgcaa acgcgtgaaa aaagattgag tttattgttt ttgcttacgc
      361 attccagctt tgagcaaatt cggtcggtgt atgtaatttc agggctcggt gaggacggaa
      421 gatattgtat tctttgcgcc acatctcaat cagcactttg gcttctctga gcgttctgaa
      481 ataggtggca ttcaggcatt cggtccgcaa tttgccattg aacgattcgc atttgccgtt
      541 ctgccagggg ctaccgggat caatgaaaat ggcagcaacg cctttctctt tcagccactt
      601 ttgtagggat ttttcaataa actctgaacc attatcgctg cgcagataac aagggtgacc
      661 ccgttctttc atgacctgag ccaaagtttc ctgcacatct tttgccttta acgagcgctc
      721 tacccggatg gttaggcact ctctggtaaa ttcatccacc accgtcagca tgcgtaatct
      781 gcttccgtgg ctcaatcggt cagccacaaa gtccaacgtc cacacatgat tcggtgcaat
      841 cgcttggcaa ggcgctaacg gaacgccttg tccctggcgt cttctcttgg gttttcgctt
      901 ggggacctgt aatttcgaca ttttccacaa tctccagatt cttttgacat tcaccaccca
      961 accttctcgt ctcaacaagg cccagatacg tcggtagcca tagcggaagt gctccgtagc
     1021 cagttccttc attctggcca cgagaggcat atctttctcg ctgggctggt agttggcagt
     1081 gtttctacca atccctgcta aaccgcaaga acggcggaag gaaagccctt gttcctggag
     1141 cattcccacc accgttctgc gcgctctggg ccttagagct tttttagaac gtctttgacc
     1201 gcctcatttt ccagcatgag atcagccacc attcttttca aacggctgtt ttccgtttcc
     1261 agctctttca atcgcctggc ttcattgacg ttcaaaccgc cgtatttttg tttccacttg
     1321 tgaaacgtgg cttgggcaat gcccatctcc cggctgattg ccacaatggt cttaccaccc
     1381 acttcgtgct cttgcagcgc tttcacgatc tgctcttcgc taaaccggct cttcttcatc
     1441 gtttgaggtc tccttgctca tgttctaaca ggaaaacctc tactttcaac tggccctttt
     1501 tattgggggc acgtcagtgc tgaacttttt tgcactgctg tcatcagcct taacagattc
     1561 agctacttga gttgttagag ggtattttaa gaaggcgcct aaagtcgcat aggttagttg
     1621 caatccacca ggctcgtatg agtgatctct tttgcatgga ccactaagac gggtcaaaat
     1681 tctaaaacct tgggcattcc cttcaaattt aaggaaatcg tctttctgcg gttgcgttaa
     1741 accatccaaa acattcttat tgtttttaaa ccagtcactt attgctttct cacctgagtg
     1801 gccaaaaggc ggattcccta tgtcatgggc caaacaggct gagtaaacga tatctccaaa
     1861 atcagtatga gaatgtcctt tagtgctgaa aggcttgtca tccttacaaa ttttatgacc
     1921 gacataattc cccagagaac ggccaacgga tgaggcttcg atactgtgcg ttaatctagt
     1981 tcgagtatag tcagtatccg gggtaggatg aacttgagtt ttggcctgta gccttcgaaa
     2041 agccgatgag aagacgattc gatcacggtc tttctcaaac tctgttctaa aaggatattc
     2101 ttctaattgg gtaggatctt catcttttcg acccagacgt ttttcacata agagtttttc
     2161 ccaatccata ataaccatca gtattcccac actctttccc gacaatgctc agtaattctt
     2221 ggtatgctga cacagaaccg acacatgata ccccaaaacc accccaaaca ctacagtttg
     2281 taaagcaccc caaagcctgt cctagtcaca agagagacga gtgggcccag caggattcga
     2341 acctgcaacc aagggattat gagttgggcc aatacccttt cctgacgagg attacagtgt
     2401 ctcgaatgat gccgatcgtt ctctttcaga cgcttttgcc tttacccact cttcctctaa
     2461 aaagctcaaa gcttcctatc ttttccatac aggccgacac agtcctgaca catgacctga
     2521 gaaatcgacc accttacccc tctggggtta ttataacaag agagtgggcc cagggggggc
     2581 cttttcgcct tctgtcttgt gagtacctac ccaggcatta aacttgggga agcctccagc
     2641 ttggtaaaac ggcggaatca ttgaaaggca ggtttcaaat gagctttaaa ctacgaaaga
     2701 agatagacca ttcaaaaaca tttatatgcg atatatacat cacagattgg gcaattgatg
     2761 ttgatttcaa tcattatgcg agcatgttcg cttccgatgg aaatggcatc ctaaaagtga
     2821 tccgttttta agcaaaacgg cgatcaaaaa gtgatccacc ctctccgggg tcggttttgg
     2881 tatgaccaag gccgaagtcg agaaccggag aggcaggccg aggtgaaaag cgtgtac
//
LOCUS       DATBTE010000273         7322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1811305, whole genome shotgun sequence.
ACCESSION   DATBTE010000273 DATBTE010000000
VERSION     DATBTE010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811305"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VIG33_10050"
     CDS             <1..436
                     /locus_tag="VIG33_10050"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HEY8271217.1"
                     /translation="DVGLLGCLSRVEWDELAANHFSGLLAEQYVAQHLSLFHGPLRTP
                     ELHYWLNDKKSHRAEVDFLLQTGSKVLPVEVKSGSPGQMKSLLTYLALHASVKRAIHL
                     GPHSFSVGKLDYKLAQEKESVRVNRQLIQVPLYLVDRLKQIL"
     gene            complement(443..1753)
                     /locus_tag="VIG33_10055"
     CDS             complement(443..1753)
                     /locus_tag="VIG33_10055"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8271218.1"
                     /translation="MKFVNRKLELDLLSESHKKNGLVVLFGRRRIGKSNLLEYWLKKS
                     KIRGCRVQCIQGTSESQIKSIYDDLKDFLNIHIEPTTWPQLFQLIDDIAGPFTLAIDE
                     FPYLVETQKSVPSIFQKWIDTKKKKDLHLVLLGSSQHMMHDIFLNKAAPLFGRAHREI
                     KLEAMSFEAFCEALDIGPTQKSFELYALVGGVPKYWEYIDAHLTVEKNIDQLFFNYAA
                     FLENEPRKLLQDEGIEGLIPLSVLDCIGRGAHKPSDIAARIGIQQSQLSRTFTALLEG
                     HFLTKDICFNEGAKNPKKVLYQIQDYSFCFWYQVYTPYQARWPRMTAEEKRHVLQLYF
                     SKIFEDYMRKQHKGAQRYWESIKIKKPNGEIIVKGIEFDAVFYDEKHPKQLVIAEIKY
                     KNLSTQQTLTLLSELKKKWEQSKLSSQYENPSFRIIDLNKINHP"
     gene            2022..3500
                     /locus_tag="VIG33_10060"
     CDS             2022..3500
                     /locus_tag="VIG33_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271219.1"
                     /translation="MNKKSLSRCMVIGLIFLSSIHAFAEGEEVAAEAATGGGPPTGPT
                     GYNTADVTKPADNIAAPAAVGVLTLADLDVQTYVKAEAEALKKNTNDYAENQEACKSR
                     EDKANYFCMESRNPDVKKYLIISQVLMAGVSGMADACGKFGQVMDAGNKALTAYQALC
                     SSWRGMCLSSCGGAVQNIQDAQKNLDILVKKATAYANNKIATLRAANPGDPKIPGYES
                     VIKNYQTQKEKLEPSLQKELEKKGDYMSVADKKTTCDGYAKELASSALGMLSMAKSFG
                     QANNCNKNTTNAPNPVAAGATPVDCTIATNKQNNMTCICQDSPRTPGCNTGLDSALSA
                     KNADSMRSAGVSDYVPGKSSAKAGVDGGSGETTLASKLDGGGGGSLPGAPSGGSAGID
                     GGNGGGGGPPGSQGAAKSSGLNTNILAGEGGGGGGGGWGGYGSDGEGKSLRQYLPGGA
                     KDPTALAGQSVVNKEVTSQGGKSNWEKVRERYRDNKPKLLGY"
     gene            3504..4466
                     /locus_tag="VIG33_10065"
     CDS             3504..4466
                     /locus_tag="VIG33_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271220.1"
                     /translation="MKNIKSFLVAFVFMSTSNSYGVSAIEVAPKGRPTPASAPAKSSI
                     TDSADKTKSSNAMGEILNMAMGAVEMAAGAYFLSTQCEPPIITTGCIMGPIMFLMGAQ
                     SFAQGGAQGKTAGQAGNTFGLTDTGLGDSSYDPSAVKALEKDPDMKTAREFMANVASG
                     KAPFTYDAKTGTLTLQDGKTLKGSDLNSAASMAAAGIPKSAIDMLGSMEKDILAKAEK
                     KVGKLNLATSSSEESSSGGGGGGSGSRASASSGEGFGARTGGSVGGLGIDRDPAQLAG
                     MQKNYNGEPIGVAGDSIFKMMTRRYKTKEAQSSFIDESEFLMQK"
     gene            complement(4503..5510)
                     /locus_tag="VIG33_10070"
     CDS             complement(4503..5510)
                     /locus_tag="VIG33_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEY8271221.1"
                     /translation="MNVMLLAAGEGTRLRPYTLIRPKPAIPFLNIPLASYSLAFLEDT
                     RIDRLVVNTFHLPTKIVDLFIHMNHGARRLHFSHEIHQLLGSGGGLGAARDYFKATED
                     LILMNADEVILPLEKGIIAKAIEQHQKNKAFCTLLTIDHEGVGTQFGGVWVDHKNRVR
                     GFGKKPPGIECVKGEHFVGIQILSGKVFEYLPNGYPSNILYDVVTRAIQDDLLIETYK
                     IPCHWYETGNLQDFLKATENVLNILTQPSHSYVQERLLQTLEKFSKEAPIIRSENGLL
                     SLRTASSVIENNVTLSGFAVLGPQCKIKASSKLKNVVVADHVQIAEGTEAQDLIFLQN
                     N"
     gene            complement(5507..6517)
                     /locus_tag="VIG33_10075"
     CDS             complement(5507..6517)
                     /locus_tag="VIG33_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY8271222.1"
                     /translation="MEEFIQSFLKNSLKSDDLKVYSLAGDASNRRYYRVIHNHQSWVL
                     MRWDPFDPENYPFLSVLQHFAKAGVHVPKVVFMSPKDGLVLLEDLGDLTLERKFLESQ
                     SQEISLDFYKMAINEIVKIHHQATATPGNCTALDIQFDTEKFLWEMNYGKDNLISGIL
                     KFQWTDSSTKEIQSIFNDICTKLDREPKKIAHRDYHSRNLMIKLDEMKVIDFQDARLG
                     PIQYDLVSLFKDSYVNMNDEMTKTLMEYYLTSSKEFLPKDFSRAHFDEIYELQSIQRC
                     FKACGSFASFYNLRNDKRYLKYLSGTLRKVIKSLTEFPEYKPFADILIDSGALERKYE
                     LL"
     gene            complement(6560..>7322)
                     /locus_tag="VIG33_10080"
     CDS             complement(6560..>7322)
                     /locus_tag="VIG33_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271223.1"
                     /translation="LFIPIIVQEQFLGAAVIHQGWELTPEMKQSAAQLVRTILEPELY
                     IEYLNRRRTNLQSAKSSNFSTDNLSSFSENFPDNFNDDFTVSDTTEEFGNYEGNDPGL
                     VAEKKTLITSLLHLQGHDNSLIKKIALQVHDFSARWAFVSFEDICNEISTEMDICNLG
                     GMTLFFENIETLAPKHQELLAEYLSSPRSLSEPLILTSSTLSLESLSERLVSDALIKD
                     IQTSHLEVQRAPLNSQMLRDLLDLFFHRDEFQDLH"
BASE COUNT         2130 a         1457 c         1672 g         2063 t
ORIGIN      
        1 tgatgtgggt ttgctcgggt gcctcagccg tgttgaatgg gatgagcttg ctgcgaatca
       61 cttttctgga ttgcttgcgg agcagtatgt ggctcagcat ctaagtcttt ttcatggccc
      121 cttaaggact cctgagttgc attactggct taatgataaa aaaagtcatc gcgcggaagt
      181 ggacttccta ttgcagacag gctcaaaagt actaccagtt gaggtgaaat ccggatctcc
      241 ggggcaaatg aaatccttat taacttactt ggcgcttcat gcttcagtaa aacgagctat
      301 ccacttggga ccgcattctt tctccgtagg aaagcttgat tataagcttg cacaggagaa
      361 agagtctgtt cgggtcaaca gacagcttat ccaagttcca ctttatcttg tggaccgatt
      421 gaagcagatt ttataagaat tcttaaggat gattaatttt attaaggtcg ataattctaa
      481 aacttggatt ttcatattgt gacgaaagct ttgactgctc ccattttttt ttgagctctg
      541 aaagtagagt cagagtttgt tgagttgaga gatttttata ctttatttct gctattacca
      601 attgctttgg atgtttctcg tcataaaata ccgcatcaaa ctcaattccc ttaacgataa
      661 tttctccatt gggtttcttt attttaatac tttcccaata gcgttgagca cctttgtgct
      721 gcttccgcat ataatcttca aatattttag agaaatagag ttgcagtaca tgccttttct
      781 cttcggcagt cattcttggc cagcgcgctt gatagggggt ataaacttgg taccaaaagc
      841 aaaaagaata atcttggatt tgatagagaa ccttctttgg attttttgct ccttcattaa
      901 aacagatatc tttggttaaa aaatgccctt caagaagtgc tgtaaaagtt cttgaaagtt
      961 gcgattgttg aattccaatc ctagctgcga tatcgctcgg tttgtgcgcc cctctgccaa
     1021 tacaatccaa tactgaaagt ggaattaaac cttctattcc ttcatcttga agcagtttac
     1081 gaggttcatt ttcgagaaat gctgcgtagt taaaaaaaag ctgatcaata tttttctcaa
     1141 cagtcaggtg ggcatctata tactcccaat atttagggac gcctccaacc aaagcgtata
     1201 actcaaatga cttttgagtc ggccctatat cgagagcctc gcaaaaagcc tcaaaactca
     1261 tggcttcaag ttttatttct ctatgggctc ttccgaaaag gggagcggct ttgtttagaa
     1321 aaatgtcatg catcatatgc tgactagatc ccaatagaac tagatgcaga tccttttttt
     1381 tcttagtgtc gatccatttt tgaaaaatgg aaggcacaga cttttgagtt tccactaaat
     1441 aagggaactc atcaatggca agagtgaaag gtcccgcgat atcgtcaata agctgaaaga
     1501 gttgtggcca ggttgtcggt tcgatgtgaa tgttaaggaa gtctttcagg tcatcataaa
     1561 ttgatttaat ttgagattct gaagttcctt gtatgcattg aaccctgcag cctcggattt
     1621 tagatttttt aagccaatat tctaatagat tcgatttacc aattctacga cgtccgaaga
     1681 gcacaaccaa tccattcttt ttgtgacttt cagacaggag atccagctca agttttcggt
     1741 taacaaactt catcttcgca ccttatcctg tttttaacat tatgcctgac aaacattatg
     1801 cacgtaaaac ataatgtttg tcagacataa tcatcttaaa tggctgaatt tctttaaagt
     1861 ccgtaaagtc tcatattgag ccagattcat agccaaagtc actaaaccaa gggcgctacg
     1921 tcattaattg agacggattt gttctaggaa caatgtttca agcttctcag acttttgtcc
     1981 gatataattg agactacgga atgttttcct ggggatgaat catgaataag aaaagcctat
     2041 ctagatgtat ggtcatcggt cttatttttc taagttcaat tcatgctttt gcagaaggcg
     2101 aagaagtggc tgcggaagcg gcaactggcg gcggacctcc aactggtcca acgggctata
     2161 atactgcgga cgtgacgaag ccagcggata acatcgcagc gccggccgca gtaggcgtcc
     2221 taactctcgc agatttagac gttcagacgt atgttaaagc agaggctgag gcccttaaaa
     2281 aaaacacgaa tgactatgcg gaaaatcagg aggcctgcaa atcacgggaa gataaagcta
     2341 actacttttg catggaaagt cgcaatcctg acgtcaaaaa atacttaata atatctcagg
     2401 tattgatggc tggtgtttcg ggaatggcgg atgcctgtgg taaatttggt caagttatgg
     2461 atgcaggaaa taaagccctt actgcatatc aagccctatg tagttcatgg agaggcatgt
     2521 gtctttcctc ttgtggtgga gcagttcaaa atattcaaga cgctcagaaa aatttagata
     2581 tcttggttaa gaaagctact gcgtatgcga ataataaaat agcaactctt cgagctgcga
     2641 atccaggaga ccctaaaata ccaggctatg agagcgtaat taagaattat cagacgcaaa
     2701 aagaaaaatt ggagccaagc ttacaaaagg aattggagaa aaaaggcgat tacatgtcgg
     2761 tcgccgataa aaaaacaacc tgtgatggtt atgcaaaaga actggcgtct tcagcactcg
     2821 gcatgttgag catggcgaag tcatttggcc aagccaacaa ttgcaataag aatacaacaa
     2881 atgcaccgaa tcctgtcgct gcaggcgcaa cgccggtgga ttgcactatc gccacgaaca
     2941 agcaaaacaa tatgacttgc atttgtcagg attctcctcg cacccctggc tgtaacacag
     3001 gtcttgatag cgccctgtct gcgaaaaatg cggactctat gcgatctgca ggcgtcagtg
     3061 actatgtacc aggaaagtcc tctgctaaag ctggtgtgga tggtgggagc ggtgaaacca
     3121 ctcttgcgag caagttagac ggtggtggcg gaggttcttt acccggagca cctagcggcg
     3181 gctcagcggg cattgatggc ggcaatggtg ggggtggcgg tcctccgggt tctcaaggcg
     3241 cagcaaagtc ttcgggtctt aataccaaca ttcttgcggg agaaggcggt ggtggcggtg
     3301 gcggaggctg gggaggctat ggatccgatg gcgaaggcaa aagcctccgt caatatcttc
     3361 ccggtggagc taaagatccg actgcgttgg caggacagag cgtggtgaat aaagaagtta
     3421 cttcgcaggg tgggaaatcc aattgggaaa aagttcggga acgctatcgt gataataaac
     3481 caaagttgtt gggatattaa attatgaaaa atataaaatc ttttctagtg gcttttgtct
     3541 tcatgtcgac ctcaaacagc tatggagttt cggctatcga agttgcacct aaaggaaggc
     3601 ccactccggc ttctgctccg gcaaagtcgt ctatcacgga ctctgcggat aaaactaagt
     3661 cctctaatgc aatgggagag atcctgaaca tggccatggg agccgtggag atggcagcgg
     3721 gagcctactt tttgtccacc cagtgtgaac ccccaataat tacaacgggc tgtataatgg
     3781 gaccgataat gtttttaatg ggtgcccaga gctttgcgca ggggggagcg caaggtaaaa
     3841 ccgctggcca agcgggtaac actttcggcc ttacggatac aggtctaggc gacagtagtt
     3901 acgatcctag tgcggtgaaa gcccttgaaa aggatcccga tatgaaaaca gcaagagaat
     3961 ttatggcgaa tgtggctagt gggaaagctc cttttactta tgatgccaag acaggcacgt
     4021 tgactctgca agatggtaaa actctcaaag gcagtgattt gaatagcgcg gcttcaatgg
     4081 ccgccgcagg tatccctaaa agcgctattg atatgctggg aagtatggaa aaagatattt
     4141 tggcgaaagc tgaaaagaag gtgggtaaac tcaatctagc tacttcttcc agtgaagaaa
     4201 gtagcagcgg tggaggcggc ggtggcagcg gttcaagagc ttcagcttcg agcggagaag
     4261 gctttggtgc ccgcacaggc ggtagtgttg gaggacttgg aatcgatcgc gatcctgctc
     4321 aattagcggg aatgcaaaaa aattataacg gtgagcctat tggggttgcc ggagatagta
     4381 tttttaaaat gatgactcgt cgatataaaa caaaagaggc gcaaagtagc tttatagatg
     4441 agtctgagtt tcttatgcaa aaataatttt caagagatta ttcagttgag cttaaattct
     4501 agttagttgt tttgtaaaaa gataagatct tgtgcttctg tgccttcagc gatttggaca
     4561 tggtcagcaa caactacgtt tttgagcttc gatgaagcct tgatcttgca ttgaggccct
     4621 aagacggcga atccagagag tgtaacattg ttttcgatca cactacttgc ggttcgcaag
     4681 ctgagcaaac cattttcaga acgaataatt ggagcttcct tgctaaattt ttccaatgtc
     4741 tggagcaagc gctcttggac atagctgtgg gaaggttgtg taagaatatt taggacattc
     4801 tctgtagctt ttaaaaagtc ttggaggttt ccggtttcgt accagtggca aggtatttta
     4861 taggtctcta ttaaaagatc atcttgaata gctctagtaa cgacatcata gagaatattt
     4921 gaggggtatc cgttgggaag atattcaaaa actttaccag aaagaatttg aatgccaaca
     4981 aaatgttccc ccttaacaca ttcaattccg ggtggttttt ttccaaatcc gcgaactcta
     5041 tttttatgat ctacccagac tccgccgaac tgtgtgccca ccccttcatg atcaatggtg
     5101 agaagggtgc aaaatgcctt gtttttttgg tgctgctcaa ttgctttagc aataattccc
     5161 ttttctaaag gcaaaatgac ttcatcggca ttcatcaaaa tcagatcttc agtggcttta
     5221 aagtaatccc gcgcggcccc cagaccacca ccactgccaa gaagctgatg gatctcatgc
     5281 gaaaaatgca atcgccgagc accatggttc atatgaataa ataaatccac aatttttgta
     5341 ggcaaatgaa aggtatttac taccaagcga tcaattctcg tatcctctaa aaaggcaaga
     5401 gagtacgatg ctagaggaat attgagaaaa ggaatcgcgg gcttgggacg aatcaatgta
     5461 taaggtcgca gtcgagtgcc ttcacctgcg gcaagaagca tgacattcat aaaagctcat
     5521 actttctttc taatgcccct gaatcaatga gaatatcggc gaagggctta tactcaggaa
     5581 actcggttaa acttttaatg acttttctaa gggtgcccga aagatatttt aaataacgtt
     5641 tatcgtttct taaattataa aaacttgcga agcttccgca ggctttaaag caacgctgaa
     5701 tgctttgcaa ttcataaatt tcatcgaaat gtgcgcgtga gaaatctttg ggcaagaatt
     5761 cctttgaaga cgtcagataa tattccatca aagttttagt catttcgtcg ttcatattaa
     5821 cataagaatc tttaaacaga cttaccaagt cgtattgaat gggccccaaa cgtgcgtctt
     5881 ggaaatcgat gactttcatc tcatcaagct tgatcattaa attgcgagaa tgataatccc
     5941 ggtgcgcgat ttttttcggc tcgcggtcga gcttcgtaca gatatcgttg aaaatgcttt
     6001 gaatttcttt tgtggagcta tccgtccact gaaactttag aataccggaa ataagattat
     6061 ctttcccata attcatttcc cataaaaact tttctgtgtc gaattggata tcaagggcgg
     6121 tgcagttgcc gggagttgcc gtggcctggt ggtgaatttt gacaatctca ttgatcgcca
     6181 ttttataaaa atctaaagag atctcttggc tctgactttc tagaaatttt ctttcaagtg
     6241 tcaagtcgcc aagatcttca agcaaaacta agccgtcttt aggagacatg aaaacaacct
     6301 tcgggacatg tacaccagcc tttgcaaagt gttggagtac acttaaaaag ggatagtttt
     6361 ctggatcaaa gggatcccat cgcataagta cccacgactg gtgattatga ataacgcgat
     6421 aatagcggcg attcgaagcg tcacctgcga gagaatagac tttaagatca tcacttttaa
     6481 gagaattttt caaaaaggat tggatgaatt cttccataaa gttcatcctt acagacagga
     6541 ccagaaaagg caagactcct tagtggaggt cttgaaactc atctctgtga aaaaacagat
     6601 ccagcaaatc tcgtaacatc tgtgagttta aaggtgcacg ctgtacttcc aaatgagagg
     6661 tctggatatc tttgattaag gcgtcactaa ctagacgttc gctcaagctt tccaaagaca
     6721 aggttgagct tgttaagatt aaaggctcag acagcgagcg gggcgagctt aaatattcgg
     6781 ctaaaagctc ctgatgtttg ggggcaaggg tttcgatgtt ttcaaaaaat agagtcatgc
     6841 cgccaagatt gcaaatatcc atttcagtgg aaatttcgtt acaaatgtcc tcgaaggaaa
     6901 caaaagccca gcgtgccgag aaatcatgaa cctgcaaggc aattttctta ataagtgaat
     6961 tgtcatgtcc ttgcaggtgc aataaagaag taataagagt tttcttttca gccacaagac
     7021 cgggatcgtt gccttcatag ttcccaaact cttctgtggt gtcagaaaca gtaaaatcat
     7081 cgttgaaatt atcaggaaaa ttctcagaaa aggagctcaa attatctgtc gaaaaatttg
     7141 aagacttcgc gctttgcaga tttgtccttc gtcggttaag atattcaata tagagttcag
     7201 gttctaatat cgtgcgaacg agttgcgccg cactctgttt catttctggt gtaagctccc
     7261 acccctggtg aataacagcg gctcctaaga attgctcttg gactatgata ggaataaata
     7321 aa
//
LOCUS       DATBTE010000274        26038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1880936, whole genome shotgun sequence.
ACCESSION   DATBTE010000274 DATBTE010000000
VERSION     DATBTE010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 26038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26038
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880936"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2176
                     /locus_tag="VIG33_10085"
     CDS             <1..2176
                     /locus_tag="VIG33_10085"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEY8271224.1"
                     /translation="SLDGSGVGAMMGTQLNGLTSGSLTLQPAAVTTNYSLTFPGAQGG
                     AGQTLSNDGSGVLSWITPLTSSSGFVNGGNTFAGNSSIGNNDNFNLDIKTNNISRMTI
                     LNNGNVGIGTTSATASFQINSTGNPSTSTILPYFNAIANPSVSVDASYNYGLLTSQTA
                     NTRELFTADYLEINPASNLTQSIYGRVMQQTVDAANTYTLGTLVGLSEFNSNFGSGNI
                     TTQQGVAITSANRGTASITNQYGLNVGATNVSGTVTNQYGANLTAFNSNTTTVTTQYG
                     AYIRASNSSTGTVTNSTAVYGISDNNTGGTVGYPIGVNGLVQNANGTVSGWAIAVNGG
                     VNNGSTMQSAYGMSGAVSNSGTLNYGSGAAFAVSQGGATTMTQATGVDAAINGTIAGG
                     TITTAYGQHIGFTNVAGSTIGSAYGLYITGLGTGGTWTNTPFDVYAADTTAYNYFAGN
                     VGIGTTAPAYKFDVLISGLVANDYYEVARFGEDTTGKGVGLGYIVNSAGTAISGAFFN
                     AQAGDAYVGTNSNYTALTVKNTTGNVGIGITSPTQALDVVGNIKTSGCLYYASSSLGT
                     CASDERLKKDVKPFDLGLKALLGLKPVNFQYNGLGGFQDDGKTQLGVIAQEVEKAAPE
                     LIKKQTVQLHPEDNEKTEIKVVDYGAFTYVIINAFKEFYHETMKRFESQDHAIASKAD
                     IAELNELKAKDLAKDQKIQKLEKENTEIRARLEKIEGAIHSK"
     gene            2341..2946
                     /locus_tag="VIG33_10090"
     CDS             2341..2946
                     /locus_tag="VIG33_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271225.1"
                     /translation="MKSSTLNYLLISLFTLSLFVACADQHSNNNNGAPTNIATVPPPA
                     NCVNGTAYCNSNQYIGNPGFSAYPNNPYYYTNRYAWNQGGYYGNFCDCPNGSRPVYNG
                     QFGLGCVAISSFQSYTGNAVYWGLGANNNQWVNIPQISNSHGYPQNGCYQQVMQSCFI
                     DQANSCGVGLTCQTTGGASRLGICVGQGATNSSLGGMGGYR"
     gene            3022..5082
                     /locus_tag="VIG33_10095"
     CDS             3022..5082
                     /locus_tag="VIG33_10095"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY8271226.1"
                     /translation="MTIQNPLLEKFSLKDQAVPFDLIKEEHFLPALDEAIKKAQQNIE
                     AIKKNKLEPSFENTIVSLETCSELVERVVGIFGNLEAAHASEAHQALAKEIYPKYTTF
                     TSDVSLDEEIFRKVKIVYDKRNSLDLTKEQLKLLEKTYLAFSRNGALLDNEGKEKLRA
                     LDQELSVLGPQFSEHVLKASNSFEMILDKKSDLEGLPEGAIEAAAHAAESKGYKGQWL
                     FNLQMPSYLPFLTYAKNRSLREKMWRAYGSRAFSQTDNKTPDKFDNQETIKKIVTLRH
                     QRAQLLGFKTHADFVLAERMAKDPSTVKDFLIKLLEPSKKAAEKDFAEVAAFAKQSEG
                     LTELKPWDFGYFAEKLKEAKYAFNEEDLRPYFKLENVVQGVFEHARRLYGLTFKETKE
                     PAVYHPEVKVYEVFEEKTGKYISLFYTDFFPRESKKDGAWMTTFRDQGLLDGTVKRPH
                     VSIVCNFTSPTPTKPSLLSYNEVKTLFHEFGHALHGMLSECTYRSLSGTNVYWDFVEL
                     PSQIMENWVGEKEGLDVFAKHYETNAPIPADLVEKIKKASQYLAGWSSLRQIQFGLLD
                     MAWYSQDPSKIANVDEFETEVTKVTHIMPKISGTNSSCSFSHIFAGGYSAGYYSYKWA
                     EVLDADAFEFFMEKGLFNSEVAQKFKDNVLSRGGTEHPMDLYKKFRGREPDPQALLRR
                     DGLI"
     gene            5082..5966
                     /locus_tag="VIG33_10100"
     CDS             5082..5966
                     /locus_tag="VIG33_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEY8271227.1"
                     /translation="MAEQKLLLKDNKNIALIYRPETTEAAQLAKKITLWLKTKGYVVF
                     TAPQQPLIPGTQAMKTKAALDKIGLAIALGGDGTYLRAVHTLEGRQIPIVGINMGSLG
                     FLTGNRVEDTFKVLEETLENKMDLRSRSMIHVLIYRKNKIRAEYHALNDVVIERGSGS
                     HLINVTIYSEKFLVSEVKADGFIIASPTGSTAYNLAAGGPILHPEAKVFVVTPVAPHS
                     LTSRPLILPDDLQMSFQMDRRSKKAQLIVDGQNETEVTPEDEVIIKRSCYDHWVVRAP
                     KHNYFHLLREKLKFGDRS"
     gene            5985..7679
                     /gene="recN"
                     /locus_tag="VIG33_10105"
     CDS             5985..7679
                     /gene="recN"
                     /locus_tag="VIG33_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY8271228.1"
                     /translation="MLLELKVSNFAIIDNLHIEFEEGLNILSGETGAGKSVLLKSLAL
                     LMGEKSSADTIRTGCQMATIEGSFDILKRPDIRKNLKDLDILVEENTLIVRRTISAGD
                     KSKVYLNGTLSTLHNLRDVVAPLIEVAGHSAPLIEMTGQNENRHLLSKAYHLDLLDQY
                     AGTWDKRLQYQKGFEHFQEIKKEIQKLENETKQKNQRLDFLTFQRDEIAVLDLSPGED
                     HEIEAEIKKLKSASRLFRFVEQAEEALYSDDGSALNRLNTMLKLGADIANLDPQLASK
                     IEVLEQAKTLIDDCVYDLRQYVNKIDGDPQRLESLEQRLSDLRKVQKKYGPSVDDILK
                     ALMEMEIEIGHLQNSETRIETLKKESTALLKELGKLASDIHERREKGAALLSDSVNKE
                     LADLNMKGVTFHVGIQKLGELSATGMSEVEFMSQTSAKDPKRPLAKFASGGELSRILL
                     SLKRVVGSSNQPRTYLFDEVDTGVSGQTAEKVGRKLKSIAKGQQVICVTHLPQVAAFG
                     DVHFFIQKAPKKDAIIMEVNELKSKDRVKEIARLISGEKISKTSLDHAEQLLKEAH"
     gene            complement(7694..8992)
                     /locus_tag="VIG33_10110"
     CDS             complement(7694..8992)
                     /locus_tag="VIG33_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271229.1"
                     /translation="MKSSIRFFHGLVQMLILGGFALNVVGCAKGGDSGSATEGIESST
                     APVSAEVALLSAKTSNRNPHHCVQTDEKCFSETFQQPVTNTRAVDVLFVVQTSDSLVS
                     EKQALVTGIDNFIQSLPANSDFNIAVMLSHGSTSPYSGKLYQAESEPIVLKSSELTPA
                     QIKTFLDVKLTQVVADPDSGGGEEGLFSLFNGITTPALLAASQAQGFFRPNAALGVIF
                     VADRRDICAVVPAGVPDETDPVKIDARIRDCEGLTAAGLSSRLHTLKGSAPLAVSGII
                     YADEPAPAGNEIGYGYTDMIALNAGVAIDIANDNIAIGLASVAQLSGQQMVIQKEFTL
                     ENNNINPKRIIVTVNGQGAAFTLNGNKVTITSEVPAGAVVVISYCLHSVENGHHDDNH
                     HDHSKGRKWWSSHGKKGHCKSKKHGHHKHLGKEKYGHWHH"
     gene            complement(9439..16101)
                     /locus_tag="VIG33_10115"
     CDS             complement(9439..16101)
                     /locus_tag="VIG33_10115"
                     /inference="COORDINATES: protein motif:HMM:NF033510.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271230.1"
                     /translation="MSQFNTLKKLLTYCGALVLATACTDTKLFFSVDPVSCQVPTSSA
                     KFGVNPGPVILYPTADTVATGQVNVTGSCVSGIPVLIQEISGKTTTVDCIKNVFATEL
                     QLSDGDGTKDLLVSQKTAADGSEIADRMCFMKDTIPPAVNISSENSAQSTNATSVPVS
                     GTCESGLPVMLWGPGLSTPVSADCKNGTFSTVIPVRPGDGIKDIVGGQVDRAGNQGLA
                     SSTLSVDTVPPKVQITSAIPLVTNLASLEVSGTCEAGLPLEIGELHATTAPAVSCANG
                     SFTANISLPVADGGALLRVFQVDTAGNLGSDSKNTIKDTVAPVVTIALPLANAYLAPT
                     STFSGACETGLPVELSGTALRGPKSIACSSGAWTLTSAITSGEGSKSLIATQTDGAGN
                     KGQVQQSFTVDSLPPVLAITSPGANSYVGANFEVVGTCEMGLTVQISGSGILPATSAS
                     CPNGTFTASVAASPLDGVKQVTATQIDLGGNTSSAERNFLRDTLAPRLTIISPVAGTS
                     VPANFVLSGSCESGLTIHFSGAGLASQASTVCSNAGAYSVNLNLSAGDGSKIIAANEV
                     DGAGNESTAKTNYLRDSTPPKVAITSPAANSPGQGDLTVSGTCEAGLNVTLAGSGVAT
                     AVVVPCSAQGDFSASIFFTAGSGVKLITAAQTDSSGNVGSDSRNFMSDLTAPTITISS
                     PAANSYLAANFTVQGTCEGGLPVTVSGPGLSTATTVPCNAGTYSASVTVSAGDGAKIV
                     KAEQTDAAGNVASTSKSYLRDTLAPVLTITSPLNGTIAQNGVLLTGACETGLTVTFSG
                     AGLSSSSSLVCAANTYQAQLTFSANDGSKVINLSETDAAGNNTQLSGTYVRDNTAPVV
                     EITTPVAETPAQNGLTVGGTCQAGLNVTLAGTGMATPVVVLCSTTGSFSADISFSSGS
                     GVKLVTASQTDSVGNTGTSSRSFARDLTSPTITITAPAANSYLSTNFTLEGACEKGLQ
                     VTVTGAGISAPVTVPCATGTYSADLSVSTGEGSKTVTAQQTDLSGNGGQASQTFLRDT
                     LAPILSIISPANGTIARNGILLAGACETGLTVAFSGVGLSSPSSVVCTSNSYQAILNF
                     SANDGSKVINVSETDAAGNSTQVSGSYVRDTTPPTVAITSPAANTQGQEGLTIGGTCE
                     AGLDVTLNGAGVLTPVVVSCSASGAFSADILFSSGGGAKVVTASQTDSVGNIGTSSRS
                     FARDLTPPGITITLPAANSYLAVNFTVQGACETGLAVIVGGTGISAATTVPCTAAAYS
                     ADVTVSAGEGSKTVTAQQTDLAGNVATTSKSYLRDTLAPVLTITSPANGTVAQNGVLL
                     TGACETGLTVAFSGAGISAPSSVVCAADAYQAQITFSANDGSKVIIVSETDAAGNKTQ
                     VSGTYVRDTSPPAVAITSPAANTQAQNGLTVGGTCEAGLNVTLNGSGVLVPVFVSCSA
                     NGIFSAEILFSPGSGAKLVTASQSDSLGNTGSSSRSFVRDLTPPAITITSPAANSYLP
                     VNFTVQGACETGLAVTVSGAGISAATTVPCNSAAYSADVTVSAGDGTKNITAQQTDSS
                     GNKGQAAKDFLRDTLAPALSITSPGNGTVAKSGVLLVGACETGLSVTFSGAGVSSPSS
                     VVCASNAYKTQLSFSAGDGNKVINVSETDAAGNSSQISGTYVRDATPPLVAITTPAAD
                     TPAQNGLTIGGTCEAGLNVTLAGTGVSTPVVVACIASGLFSGDITFTSGGGAKVVTAS
                     QTDGVGNTGSSSRSFVRDLTPPTVTLTSPAANSYLPTNFTVQGACETGLAVTVSGAGL
                     STPATVPCTSGAYSAVVTVSIGEGTKNIIAQQTDLSGNGGQASQNFLRDSVAPVLIIT
                     SPAPSSSVPISTNLIGTCETGITVMITGDLNSSSGSCVSGAFNIPVTFSTGLGGKNVL
                     AKQTDMAGNVGQANATYSRDSNGTSETFVSDASFGKIDILFIDDNSASMDPKQASLGT
                     KFSGFVTELLKIDWQIGITTTDCSAGPYGICGSLLPLTGINSKILTPSVANYQTVFNN
                     SIQRPETAGCLSTGTCPSGNSEPLLSATTAMNKYNTDNNGFFRANADLAIVILSDADE
                     RQTGGANYANRPTQLVAAFNNIWPSDKKLKVYSIIVKPGDASCLSSMKAGSGGFSFFG
                     DLIDQTVNLTSGLSTSICAPDYSLTLKGIGESVRTLTNSVELAHTPVANSVVVSFTPN
                     QSIGFKVIGNKVVFDSPPSVGTEIKVDYQY"
     gene            complement(16326..17843)
                     /locus_tag="VIG33_10120"
     CDS             complement(16326..17843)
                     /locus_tag="VIG33_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY8271231.1"
                     /translation="MKIFILFSLFLGFSFSQSQALTLDKYKKKPKLVVVLVIDQFRAD
                     YLTKYQKEFVLPEAKGEVGGFNYLMRKGAYFPFAQYDVFQSMTCPGHAMISTGAHPYF
                     TGISLNDWYEVGTRKKIYCAEDAEFGVSPRHLKTSTFSDELKNISEASKVVSVALKDR
                     AAVMLGGHRSDIALWMDEKYFNWTTSKYYQKEIPTWVKDINENLRKAGILKKDDKAEN
                     KKRLITVYGAQITREIAEAALKSENLGKNGRTDILAVSFSSHDMAGHIYGPNSAELKT
                     MTLAEDKEISQLLSKLKKHLGSLDEVVIALTGDHGVAPSVEYATNAKLESGYIDYLAM
                     YKKIYERLNHKFGEPTKEWIAAAINFNFYLNPEALQERKVSSDLVEAEIKNVMQTFPG
                     VFQVATRSEIAKGLLPVGELGQQLVRQYIAGKNGDVILIPRPFYISKGESVVNHMTGY
                     SYDSTVPLILLGPEIKSGVYSSAAKVIDLAPTLSFIMGTLPPATATGRVLSEIFE"
     gene            complement(17861..18325)
                     /locus_tag="VIG33_10125"
     CDS             complement(17861..18325)
                     /locus_tag="VIG33_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271232.1"
                     /translation="MENFRQSHKHSGFKRFFLNCARKAMILALLLSGACAHHRDVRPG
                     SDGIHSVRITSEDKEAGSREAIEQANDFCKKYEKMAVFLNEESKYEGSMSESDYKTAK
                     TTAKVAQAVGGAGYVFGGKNERSAGGILGLGGGIASEALGKGYSVNMKFKCQ"
     gene            18879..19607
                     /locus_tag="VIG33_10130"
     CDS             18879..19607
                     /locus_tag="VIG33_10130"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEY8271233.1"
                     /translation="MIQRTEKEAPLKQEMLESVKGKTTKQAKASLTAQHPVASLPLNQ
                     EKPLSATHTQILLTLDQETLKLFKEAKNLLSHSIADGNLSEVLKAISNIAIEHLAKKK
                     GRTPPKIKTETPLTSSKGKTKKAIAEAEVGEKERGVEKDAVETKEKVAESPTSEKQSV
                     THNSRYLSQETKRIVFARAGGCCEFVGSEGHRCTSTFQLEIDHLEPWSQGGSNRAENL
                     SLKCKSHNFYRAKETHGFFYKAQK"
     gene            complement(19936..20151)
                     /locus_tag="VIG33_10135"
     CDS             complement(19936..20151)
                     /locus_tag="VIG33_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271234.1"
                     /translation="MIQPKCVGCHGGSGGYTFSTYAGVMKAVVAGNAASSKLYTKVKS
                     GHPSATTLTSQELQNIANWINAGALNN"
     gene            20156..20785
                     /locus_tag="VIG33_10140"
     CDS             20156..20785
                     /locus_tag="VIG33_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271235.1"
                     /translation="MKMLVKVALLLTGVGAGVGFGVGAGVGAGVEAGVGLGVGAGVGA
                     GVGFGVGAGVGAGVEVGVGLGVGAGVGAGVGFGVGAGVGAGVGAGVEAGVGLGVGAGV
                     GAGVGAGVGAGVGAGVGFGVGAGVGTGAGTTPLKQALSFFRNADNCSFIAKHSVEPSS
                     LEQISLACARKADLWVSISSTQTRMLSRLGLEEETLVRDSCLSSDLVFA"
     gene            21322..21933
                     /locus_tag="VIG33_10145"
     CDS             21322..21933
                     /locus_tag="VIG33_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271236.1"
                     /translation="MREDRIIVMYLIFTLLFGGGILLKKIIHRIPIANSQSVTKEKDP
                     YKYSHQNPNQTTSQPIPRVPPPQPTETRQIASIPKNRPQSHPSEPLRKFSQQTLAASR
                     YSNDYDEGHDLSQKLDSLDYQERKEMDFLKMKVGLSEFEIQTLEKKRHDYQAQLQQFS
                     TQEQQGSKEAGISKQSLIKAHIQWMANYLGPENYYEFLNLSTE"
     gene            complement(21919..23031)
                     /locus_tag="VIG33_10150"
     CDS             complement(21919..23031)
                     /locus_tag="VIG33_10150"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY8271237.1"
                     /translation="MLTMEKPYVRELTGLRGWAALWVTFFHLQYGFNFSPDFYFLLFP
                     SRFIQKGYLAVDIFFILSGYVMALTCASWEGRNPFDFFKSFIWRRLARIYPLHLFSLL
                     LMMGLALFSVPTVPWEQWGRSILAHLTLTHSWGEFYTPDWNYPSWSISAEWAAYLSFP
                     LLLLLNRKVSRSLSGIAAAVILPMAALIWLAYSLKEGSMDMTWDWGWYRCLIEFWIGL
                     SLWRLGQMLGPMLTVSIKKSLGTVLTVIAAISLGLFLESPLPDPVIIPLATVLIFSVV
                     LGSRLWTFILGNRVSNYLGEISYSLYMLHVPVGLTLLKLWRYKFGELQSAISGFVALT
                     VTLMVLFLVASLMHHYFEKPLRQWMTQSRSTFLFRR"
     gene            complement(23025..23993)
                     /gene="hemH"
                     /locus_tag="VIG33_10155"
     CDS             complement(23025..23993)
                     /gene="hemH"
                     /locus_tag="VIG33_10155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY8271238.1"
                     /translation="MKLLKGVLLSNIGTPASYQPKDVAVYLREFLMDEEILPIPFLFR
                     YLLVHVGIVPRRADASAAKYKAIWTSQGSPLMVNSLLQKENLQKALGNEYVVELGMRY
                     GQPSLQQALQKFKELDVEEVYFLPLYPQYCRATTESSFKKVDELIKQHQYFFRLQKVL
                     PFWDQKEFIHAVAEETQKNLQGKKLDYYLMTYHGLPESQNRKMDPENAYREQCIKNSA
                     LLAEALGLSKEQWGFSFQSRVGVETWIKPYTSDELKALPGRGVKSLAVLCPSFVSDCL
                     ETLEEIGLEGEETFKQAGGESYHLVPCLNGRVDYLTQLIEKMPSKC"
     gene            complement(23980..25182)
                     /locus_tag="VIG33_10160"
     CDS             complement(23980..25182)
                     /locus_tag="VIG33_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8271239.1"
                     /translation="MKKVVVVGGGFSGLVTAYYLSRQGFSVDLHEASPKLGGLLQTER
                     TSSGLVENAANGFIFNEDLAELLADLKLEYIRPLKGAKKRFIFRKRLKRWPLTIFESL
                     KLFFKVIPRMIFRRASLKPQAQETMWAWGLRNFTRGLTKYLLAPVLQGIYAGNVEQMS
                     AELIIGPYFKRKNPKTKRGYRGTISFQNGMGEFIAALERKLRQEGVRIYLNSVYEPED
                     LTIPHVICVSAAVAPRVVRKVSAELASSLSRIEVLPVLSATLFFKEPQAKIVGFGALF
                     PEDQNFRVLGVLSNAYIFENRGPAYSETWIFGGAHSPEMVAKSPDEILARIQMERKRI
                     LGVEDRYFDYRMNPSPQALPHYTLEHKKILEELKLPKNLYLNGNYMGGIGLSKILSRS
                     KELVHEIT"
     gene            complement(25179..>26038)
                     /locus_tag="VIG33_10165"
     CDS             complement(25179..>26038)
                     /locus_tag="VIG33_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY8271240.1"
                     /translation="DRVSMGVQDFNPEVQKLVNRFQPYEITANLTQQARDLGFHSVNY
                     DLIYGLAKQTPESFAETARLTVALKPDRIALYSFALVPWIKPAQRLFKDEDLPSGAEK
                     RKLYEIAREILMNAGYVEIGMDHFALPSEALSLAAQNKKLHRNFMGYTDQKSEVMLGL
                     GVSSISETPYSFHQNEKLLNLYQERLEKNEIPTHRGHVHTDEDREHREQILKLMTQLR
                     VSFKNAQQANAARVFLAEMAKDGLVEVNEQEVKITEKGRPFLRNACVFFDERLRLKQP
                     QTQVFSKSI"
BASE COUNT         7255 a         6218 c         5835 g         6730 t
ORIGIN      
        1 ttctctggat ggcagtggcg tgggtgccat gatggggact cagttaaatg gcctcacttc
       61 aggttcactg actctgcaac ctgcggcggt cactaccaat tactccctga ctttccctgg
      121 tgctcaaggg ggagcaggac aaactttatc taatgatggc tctggagttt taagctggat
      181 aacaccttta acttcaagct caggttttgt aaacggtggc aacacttttg ctggcaactc
      241 aagcatagga aataatgaca acttcaattt ggatatcaaa acaaataata tcagtcgaat
      301 gacaattctt aataatggca acgtcggcat cgggacgact tcggcaacgg ccagttttca
      361 aattaacagc acaggcaacc cgtcaacctc gacaatcctc ccgtacttta atgccatcgc
      421 gaatccttca gtaagcgtcg acgctagcta taattatggc ctgttaacca gtcaaactgc
      481 aaacacaaga gaattattta ctgctgatta cttagaaatc aacccagcct cgaatttaac
      541 tcagtctatt tatggtcgag ttatgcaaca aacagttgat gctgcaaaca cttacacact
      601 tggaacgctg gtaggtttat cagagtttaa tagtaacttt ggatcaggaa atatcacaac
      661 ccaacaaggc gtcgcgatta cctctgccaa tagaggtact gcttcaataa ccaatcagta
      721 tggattaaat gttggcgcga ctaatgttag cggaaccgta acgaatcagt atggtgcaaa
      781 ccttactgcc tttaatagca acacgacaac agttacaact cagtatggcg cctatataag
      841 ggcaagtaat tctagtacag gtactgtaac taattccact gcggtctacg gtatctctga
      901 taataatact ggtggaaccg tagggtaccc tattggtgtt aacggcttgg tgcaaaatgc
      961 taacggtact gttagtggat gggctatcgc tgtgaatggt ggagtgaaca atggatcaac
     1021 aatgcaatca gcctacggca tgtcgggggc tgtcagtaat agcggaacgc taaactacgg
     1081 ttctggggct gcttttgcgg tttctcaagg tggagctacg actatgactc aggctaccgg
     1141 cgttgacgct gccatcaatg gcactatcgc cggtggaact attacaacgg catatggtca
     1201 acatatcggt tttacgaatg tcgcgggctc tacaatagga agtgcctacg gtctttatat
     1261 cacgggactg gggactggcg ggacatggac taacacgcct tttgatgtct atgctgctga
     1321 caccacagct tacaactact tcgccggcaa cgtcggcatc ggcacaaccg ctcctgccta
     1381 taaattcgat gttctcatat ctggtcttgt cgccaatgat tattatgagg tggcccgctt
     1441 cggcgaagac actactggaa aaggagtggg gctgggatat atcgttaatt ccgccggaac
     1501 cgcaatcagt ggagcctttt tcaatgctca ggcgggcgat gcctatgttg gtacaaacag
     1561 caattacacc gctctcaccg ttaagaatac gactggaaat gtcgggattg gcataacttc
     1621 acccactcaa gccctcgatg tcgtcggcaa tatcaaaacg agtggctgtc tttactatgc
     1681 gtcttcaagc ttgggtacct gtgcttcgga tgaacgcctc aaaaaagacg ttaaaccttt
     1741 tgaccttggc ctcaaggccc tgcttggctt gaagcctgtt aacttccaat ataacggtct
     1801 tggtggcttc caggacgatg gaaaaactca gcttggagtc atagcccaag aggtggagaa
     1861 agcggctcct gaacttatca aaaagcaaac agttcaattg catccagagg ataatgagaa
     1921 aaccgagatc aaggttgttg actatggtgc ctttacttat gtgatcatca acgcatttaa
     1981 agaattttat catgaaacta tgaaacgttt tgagtctcag gatcacgcta ttgcttctaa
     2041 ggctgatata gctgagctca atgaattgaa agcgaaggat ttagccaaag atcagaaaat
     2101 ccaaaaactt gaaaaagaaa acactgagat cagggctcgg cttgaaaaga tcgagggggc
     2161 gatccattcc aaataaaaaa ctctggcccc ccccctcaaa ctgattcgtc agctttttga
     2221 agaattctct cattcctttc aattttacaa ggaccttaca aaatgtgtct ctggcacgct
     2281 gcttgtagta tacagctctc ggtcgggccc caggcctctc taagcacgta aggagtcctt
     2341 atgaaatcat cgactttaaa ctaccttctg atatctctgt ttacgctttc tctctttgtt
     2401 gcctgcgctg accaacactc taacaataat aatggcgccc ccaccaatat cgcgaccgtc
     2461 cctcctcctg caaactgtgt gaacggaacg gcttattgca actccaatca atatatcggc
     2521 aaccctggct tttccgctta tcccaacaat ccctattact ataccaaccg ctatgcctgg
     2581 aaccagggtg ggtactatgg aaacttttgt gactgcccca acggatctcg tcccgtctac
     2641 aatggtcaat ttggtttagg ctgtgtggcc atttccagct tccaatctta tacaggaaac
     2701 gctgtttact ggggccttgg tgcaaataat aatcagtggg tcaatattcc acaaatcagt
     2761 aactctcatg gctatcccca gaatgggtgc tatcagcagg tcatgcaaag ctgcttcatc
     2821 gaccaagcca attcttgtgg cgtcggcttg acttgccaga ccacaggagg ggcctcccgt
     2881 cttggaattt gtgttggcca aggtgcaaca aattcgtctc ttgggggcat gggcggatat
     2941 cgttaattga agcttcatta atacggcgca ctaggcgccg taaatgctga tgggcacagg
     3001 cttttatgct acacaaagcc tatgacaata caaaatccct tactcgaaaa attttcttta
     3061 aaagaccaag ctgttccctt cgatttaatc aaagaggaac atttccttcc agctctggat
     3121 gaggctatta aaaaagctca acagaatatt gaagccatca aaaaaaataa actagagccc
     3181 agctttgaga atactattgt aagccttgaa acttgcagcg aactcgttga acgcgtggtg
     3241 ggtatttttg gcaatcttga agccgcccat gccagcgaag cccatcaagc tttggcaaaa
     3301 gaaatatatc ctaaatacac aactttcacc tctgatgtca gtctcgacga agagatcttc
     3361 agaaaagtta aaatcgtata tgataaacgc aactctttgg atctcaccaa agagcaattg
     3421 aaacttcttg aaaaaactta tttagctttt tcccggaacg gagctttgct ggacaatgaa
     3481 ggcaaagaaa aacttcgcgc cctcgatcag gaattgtccg ttttaggccc tcaattctca
     3541 gaacatgttt tgaaagccag caacagtttt gaaatgattt tggataaaaa atctgatctt
     3601 gagggactcc ccgagggagc tattgaagcc gccgcccacg cagcggagtc taagggctac
     3661 aagggccaat ggctttttaa tttgcaaatg ccaagctact tgcctttttt aacttacgcg
     3721 aagaatcgtt ctttgcgtga aaaaatgtgg cgcgcttatg gctctcgtgc ctttagtcaa
     3781 acggacaaca aaacaccgga caagtttgat aaccaagaga cgataaaaaa aatcgtcact
     3841 ctccgccatc aacgggctca acttttaggt tttaaaactc atgcggactt tgttttagct
     3901 gaaagaatgg ctaaagaccc ttccaccgta aaagatttct taataaaact tttagaacct
     3961 tctaagaaag cggctgaaaa agactttgcg gaagtcgctg ctttcgcaaa acaatctgaa
     4021 ggccttactg aacttaagcc ttgggacttc ggctatttcg cggagaaatt aaaagaggcc
     4081 aagtatgcct ttaacgaaga agatcttcgc ccttacttta aactggaaaa tgttgttcaa
     4141 ggtgtctttg aacatgctcg ccgtctttac ggattgacat ttaaagaaac caaggaaccc
     4201 gccgtttatc acccggaagt gaaagtttat gaagtctttg aagaaaaaac cggtaaatac
     4261 atcagccttt tctatacgga ctttttccca cgggagtcta aaaaagatgg agcctggatg
     4321 acgactttcc gtgatcaagg gctcttggac gggactgtaa agcgccccca tgtcagcatc
     4381 gtttgcaact tcacctctcc gacgccaacg aaaccatctc tgctttccta taatgaagta
     4441 aaaactttgt ttcatgaatt cggtcatgcc ttgcatggca tgctctctga atgcacttac
     4501 cgttccttga gcgggaccaa tgtttattgg gacttcgtgg agctgccttc tcagatcatg
     4561 gaaaactggg tgggagaaaa agaagggttg gatgtctttg ccaaacacta tgaaaccaat
     4621 gcgcctattc cagcggactt ggtggagaaa ataaaaaagg cttctcaata tttggcgggt
     4681 tggtcttccc tgagacaaat tcagtttggg cttttagata tggcttggta ctcacaggat
     4741 ccgagcaaaa tcgccaatgt tgatgaattt gaaacagaag tgactaaagt gactcacatt
     4801 atgccaaaga tttccggaac gaacagttct tgttctttca gccatatctt cgccggcggt
     4861 tactctgcag gctactatag ctataagtgg gctgaggttt tggatgccga tgcctttgag
     4921 ttctttatgg aaaaaggtct gttcaatagt gaagtggcgc aaaaattcaa ggacaacgtc
     4981 ttaagccgcg gtggcacgga acatccgatg gatctttata aaaagtttcg tggccgtgaa
     5041 cctgatcccc aagccctttt acgaagggac gggctgattt aatggccgag cagaaacttt
     5101 tactcaagga taataaaaat atcgctctga tctatcggcc tgaaaccacg gaggcggccc
     5161 agcttgcaaa gaaaatcacc ctgtggttga aaaccaaggg ttatgttgtt tttacggctc
     5221 cgcaacagcc cttgattccc ggcactcagg ccatgaaaac caaagcggct ttggataaaa
     5281 ttggcctggc aattgctctt ggaggagatg gaacttattt acgggcggta cacactctgg
     5341 aaggtcgaca gattccgatc gtcggaatca atatgggatc tctcggattt ctgaccggaa
     5401 accgggttga agacactttt aaggttttag aggaaacctt ggaaaataaa atggatttac
     5461 gttcacgctc gatgattcac gtgcttattt atcgcaaaaa taaaattcgc gctgaatatc
     5521 atgctttgaa tgatgtggtc atcgaacgag gatccggcag ccatttgatc aatgtcacga
     5581 tttacagcga gaagttcctg gtgagtgaag tgaaagccga tggatttatt atcgcaagcc
     5641 ccaccggctc cacggcctat aatttagcgg cggggggccc catcctccat cccgaagcta
     5701 aagttttcgt ggtcactcca gtggcgcctc acagtttgac aagtcggccg ttgattttac
     5761 ctgatgattt gcagatgtct tttcaaatgg acaggcgatc caagaaggct cagttaatcg
     5821 tggacggtca aaatgaaacc gaggtcactc cagaggatga agttattatt aaacgcagtt
     5881 gttatgatca ctgggtggtg cgtgctccta aacataacta cttccacctt ttgcgggaga
     5941 agttgaaatt cggagaccgc tcctaaggtg tataaggggg atgtatgtta ctagagctga
     6001 aagtttcaaa ttttgccatc attgataacc ttcacattga atttgaagag ggtttgaata
     6061 ttttgagcgg tgaaaccgga gctggaaaat ccgtcctctt aaaaagtttg gctcttttaa
     6121 tgggcgagaa atcctctgcg gatacgatcc gcaccggctg tcaaatggcc accatcgaag
     6181 ggtcttttga tattttgaaa cgtcccgata ttcgtaaaaa cctcaaagac ttggatatcc
     6241 ttgtggaaga aaacaccttg atcgttcgcc gcaccatcag cgccggtgat aaatccaaag
     6301 tttacttgaa tggcacgctc agtacgcttc acaacctgcg ggatgtagta gctccgttga
     6361 tcgaagtggc cgggcactcg gctccgctca ttgaaatgac aggccaaaat gaaaaccgcc
     6421 acttgctatc aaaggcttac catttagatc ttctcgatca gtatgccgga acttgggaca
     6481 aacgacttca atatcaaaaa ggcttcgagc acttccaaga gataaaaaaa gaaattcaaa
     6541 agctggaaaa cgaaacgaaa caaaaaaacc aacgcttgga ctttttgact ttccagcgcg
     6601 atgaaattgc ggttctcgat ctttccccgg gggaagacca cgaaattgaa gcggagatca
     6661 aaaagctgaa gagcgcttcc cgtctgtttc gattcgtaga gcaagccgaa gaagcgctct
     6721 actcggatga tggctccgct ctgaatcgtc taaatacaat gctgaaattg ggagcggata
     6781 tcgccaatct tgatcctcag ctggccagta agattgaggt tctggaacag gcaaagactt
     6841 taattgatga ttgtgtctat gatcttcgcc aatatgtgaa taaaatagat ggcgatcctc
     6901 aacgtctgga aagtcttgag cagcgcctga gtgatctgcg caaagttcag aaaaaatacg
     6961 gtcctagtgt tgatgatatt ttaaaagctt tgatggaaat ggaaatcgag attggtcatt
     7021 tgcaaaactc tgaaactcgc attgaaactc ttaaaaaaga atccacggct ttactcaaag
     7081 agttaggaaa attggcctcc gatatccatg aacgccgcga aaagggggcg gccctgcttt
     7141 ctgacagcgt gaataaagaa ctggctgacc tcaacatgaa aggggttact tttcatgtgg
     7201 gcatccaaaa gcttggggag ctttcagcca cgggcatgag cgaagtcgaa ttcatgagcc
     7261 agacctccgc aaaagaccct aaacgccctc tggcgaagtt tgccagcggt ggagaactgt
     7321 cccgcatcct gttgtcttta aagcgggtcg tgggctcctc aaatcagccc cgtacttatc
     7381 tcttcgatga agtagacaca ggggtttcag gccaaaccgc ggagaaagta ggccgcaagt
     7441 taaagagtat tgctaaaggg caacaagtca tttgcgtaac ccatctaccg caggttgccg
     7501 ccttcggaga tgtccacttt tttatccaga aggctccgaa aaaagacgcc atcatcatgg
     7561 aagtgaatga gcttaaaagc aaagatcggg tcaaagaaat cgcgcgtttg atctcaggtg
     7621 aaaaaatttc taaaaccagc cttgatcatg cggaacaact gcttaaagaa gctcactgaa
     7681 gttcaagaac aacttagtga tgccagtgac catacttttc tttgccaagg tgtttgtgat
     7741 gaccatgttt tttgctcttg caatgacctt tcttgccgtg tgaactccac cactttcgac
     7801 cttttgagtg atcatgatga ttgtcatcat gatggccatt ttcaacagag tgcagacagt
     7861 aagaaatcac gaccacggcg ccggcgggaa cctcgctcgt aatagtcact ttgttgccat
     7921 tgagggtaaa agcagccccc tgcccgttca ctgtaacgat gattcttttt ggattaatat
     7981 tgttgttctc aagagtaaac tctttttgaa taaccatttg ctgaccgctg agttgcgcga
     8041 cggaggcaag gcctatcgca atattatcat ttgcgatatc aatagccact ccagcattta
     8101 aagcgatcat atcagtatag ccatagccga tttcatttcc cgcaggggct ggctcatccg
     8161 catagatgat tcctgacacc gccagcggcg cactgccctt cagggtgtga agtcgactgc
     8221 tcagacctgc cgccgttaag ccctcacaat cacgaattct agcatcgatt ttcacagggt
     8281 cggtttcatc aggcactcct gctggaacca cggcacagat gtctcttcta tcagccacaa
     8341 aaatcacacc cagtgcagcg ttgggcctga agaagccttg cgcttgcgat gctgccaaaa
     8401 gagccggagt tgtaatcccg ttaaaaagag agaaaagacc ctcctcgccg ccaccagagt
     8461 ctggatcagc tacgacctgg gttaacttca catccaggaa ggttttgatc tgtgcagggg
     8521 ttaactctga agatttcaat acaattggct ccgattcggc ttgatataat ttgcctgaat
     8581 aaggagaagt actgccgtga gaaagcatca ccgcaatatt gaaatccgaa tttgcgggaa
     8641 gagattggat aaagttatcg attccagtca cgagggcttg tttttcagaa acaagcgagt
     8701 cagaagtctg caccacaaag agaacatcca cagctctcgt atttgtgacg ggctgttgaa
     8761 aggtttcgct aaaacacttt tcatctgttt gcacacaatg atgaggattt ctattagaag
     8821 tttttgcgga aagcagcgca acctccgccg aaaccggcgc cgtagaggac tctattccct
     8881 cagttgcaga tcctgaatct ccgccttttg cacatccaac cacgttgagt gcaaagcctc
     8941 ccagtattaa catctgaacc aatccatgga aaaaccgaat cgaacttttc atttaatcct
     9001 cctgattaac cgtttgtcat tacgcttagg taaagcaacg gccatgccag aaccaaatac
     9061 gaattcgttg ccccctcaat tgagttttgt gtttaaagaa aagacagctg ataagccgaa
     9121 atcagctctg gagcaaaagg caagcaaaag ggggttctag cagcgacaga aaaggcgcaa
     9181 aacaagccct cttcatagag ggtaaatcgc gaagagaata agagggtgtg tctcagaaaa
     9241 agacataata agggagcacg gtttagcctc tgtgattttc tattcgacct cgttgtgggg
     9301 agaagtacgc tcaatcaaag attggatacc attatttgtt atttaatgct tattcttcag
     9361 tgttttttat ccagcatcaa tgttttacca attcatgtat tcggcctcgc cgtcaaaaaa
     9421 cgaagtcgac cctaatacct aatattggta atccacttta atctcagtcc caacagaggg
     9481 gggcgaatca aagaccacct tattgccaat caccttaaag ccaatgcttt ggtttggcgt
     9541 gaaggacaca accacagagt tggcaaccgg cgtatgagcg agttccactg agttcgtcaa
     9601 ggtgcgtaca ctttcaccga tacccttcaa cgtcaggcta taatctgggg cacagatgct
     9661 ggtgcttaaa ccgctggtca gatttacagt ttgatcaatg aggtctccaa agaagctaaa
     9721 tccaccgctg cccgctttca tgctggacaa acaagaggca tcgcctggtt tcacaataat
     9781 gctatagacc tttaattttt tatcacttgg ccatatattg ttgaatgcgg cgactaactg
     9841 ggtaggtcga ttggcgtagt tcgcaccacc tgtttgtcgc tcatccgcat cactcaggat
     9901 tacgattgcc agatccgcat ttgcccggaa gaatccgtta ttgtctgtat tgtatttatt
     9961 cattgccgtc gtcgctgata aaagcggttc gctatttcca gaaggacaag ttcctgtcga
    10021 caagcaaccg gcggtttcgg gacgttgaat ggagttgttg aaaacagttt gatagttcgc
    10081 cactgaaggc gttaaaatct tactgttaat gcctgtcaac gggagcaggg atccgcaaat
    10141 tccataagga ccggcactgc aatctgtggt ggtaatcccg atttgccaat caatttttaa
    10201 cagctctgtc acgaaaccag agaatttcgt acccaaggat gcctgcttag gatccattga
    10261 agccgagttg tcatcaatga acagaatatc aatcttacca aaagaggcat cactcacaaa
    10321 ggtctcagag gtgccattgg aatctctgga ataagtggca tttgcctgtc ccacattacc
    10381 tgccatatca gtctgcttag cgagaacatt cttacccccc aggcctgtac taaaggtcac
    10441 gggtatatta aaggctcccg acacgcacga tcctgaggag ctgttaagat ccccagtgat
    10501 catgacggtg atccctgttt cacaggtgcc tatcagattc gtcgatattg gaacactgga
    10561 actcggcgcc ggagaagtaa tgatcaatac tggagcgacg ctatcgcgta agaaattttg
    10621 agaagcctgc cccccattgc cggaaagatc cgtttgttga gcgatgatat ttttagttcc
    10681 ttcgcctatg gaaaccgtta cgactgcgga ataagcgcct gaggtgcacg gaaccgtggc
    10741 tggtgtactg agaccggctc cactgacagt gacggcaagt cctgtttcgc aagccccttg
    10801 cacggtgaag tttgttggca aatagctgtt cgccgcaggt gatgtaagag tcactgtggg
    10861 aggcgtgaga tcccgaacaa agctgcggga actagaacca gtattgccaa ctccatcggt
    10921 ttgcgaagcc gtgaccactt ttgctccgcc accggaggtg aaggtgatat cgccactgaa
    10981 caagccgctg gctatacaag caacgacgac gggagtagag actcccgttc cggctaaggt
    11041 cacattaaga ccggcctcac aagttccgcc aatagttaaa ccgttctgcg cgggcgtgtc
    11101 cgctgctgga gtcgtgatcg ccacaagcgg tggagtcgca tcacgcacat aggttcccga
    11161 aatctgagaa ctattgcccg cggcatctgt ttcactcaca ttaatcactt tatttccgtc
    11221 accagcactg aagctcagtt gagtcttgta agcatttgaa gcgcacacga ctgaacttgg
    11281 agatgaaact cccgctcctg agaaggtcac gctcaatcca gtctcacagg cacccactaa
    11341 aagaactcca cttttggcaa cagttccatt ccctggagaa gtaatgctta aggctggagc
    11401 caaagtatcc cgcaggaaat cttttgcggc ttgaccctta ttgcctgaag aatccgtttg
    11461 ctgtgcggtt atatttttag tcccgtcacc ggctgaaact gtgacgtccg cagagtaggc
    11521 tgctgagtta cacggaacag tcgtggcagc actgataccg gctccgctga cagtgacggc
    11581 aagtcctgtt tcgcaagcgc cttgcacggt aaagtttaca ggcaaataac tatttgctgc
    11641 gggagaagtg atggtaatgg cgggaggagt taaatcacgg acaaagctgc gggaactaga
    11701 accagtattg cctaagctat ctgattgcga agctgtgaca agttttgctc cactgccagg
    11761 actgaagagg atctccgcac taaatattcc gttcgctgag caactcacaa aaaccggaac
    11821 taatactcca gatccgttca aggttacatt gaggcctgct tcacaagttc cgccaacagt
    11881 taaaccgttc tgcgcctggg tgttcgctgc cggagaagtg atcgccactg ctggcggaga
    11941 ggtatcgcgc acataagtcc cagagacctg agtcttgttg ccagcggcat cggtttcact
    12001 cacgatgata actttactgc catcattcgc actgaaagtg atttgagctt gataagcatc
    12061 agccgcgcac accactgagc tgggcgctga aattcccgcc cctgagaagg ccacagtcag
    12121 tcctgtttcg caagctccgg ttaacaaaac tccattttgt gcaactgttc catttgctgg
    12181 agaagtgata gttaacacgg gagcaagagt atcacgaaga taacttttcg aagtggtcgc
    12241 gacattcccg gccaaatctg tttgctgggc cgtcacagtt ttcgaacctt cacctgctga
    12301 aaccgtgaca tccgcagaat aagcggcggc agtacaaggt actgtcgtgg cggcactgat
    12361 tcctgttcca ccgactatca ctgcaaggcc ggtttcgcaa gcgccttgca cggtgaagtt
    12421 tacagccaaa taactattcg ctgctggtaa agtgattgta atgcctggag gtgttagatc
    12481 gcgggcaaag ctgcgagaac tcgtcccgat atttccaacg ctatctgttt gcgaagccgt
    12541 tacaactttt gcgccgccgc cagaagagaa gaggatgtct gcactgaagg ctccgcttgc
    12601 ggagcaactg accacgaccg gagtcaacac cccggctccg ttcaaggtga catcaaggcc
    12661 tgcttcacaa gttccaccaa tggttaaccc ctcttgtccc tgagtatttg cggcaggaga
    12721 agtgatcgcc accgtaggag gagtggtatc gcgcacatag gatccagata cttgagtact
    12781 gttacccgcc gcatcggttt cactcacgtt gatcacttta cttccatcat tcgcactaaa
    12841 attcaagatg gcttgatagg aatttgaagt gcaaaccact gaactcggtg aggagagtcc
    12901 gactccagag aaggccaccg tcaatcctgt ttcgcaagct ccggccaaaa gaattccatt
    12961 tcgcgctatc gttccattcg ctggagaaat aatacttaat atcggagcaa gggtgtcacg
    13021 taagaaagtt tgtgaggctt ggcccccatt gcctgaaagg tctgtttgtt gagccgtgac
    13081 ggttttcgaa ccctcacctg ttgaaacact taggtccgcg gaataagttc cagtggcaca
    13141 cggaactgtc accggcgcgc taatcccggc gccggtgaca gtgacctgaa gacctttctc
    13201 gcacgccccc tccaaggtga agttcgtgga taaataacta ttcgctgcag gcgccgtgat
    13261 ggtaatggtt ggcgacgtta gatcacgggc aaagctgcga gaactcgtgc ccgtattccc
    13321 aacactatct gtttgcgaag ccgtgaccaa ttttacgcca ctaccagaac tgaagctgat
    13381 atccgcactg aaagaacccg ttgtgctaca aagaaccacg actggagtgg ccattccggt
    13441 accagccaaa gtcacattga ggcccgcttg gcaagttccg ccaacggtta agccgttttg
    13501 tgccggcgtc tccgcaactg gtgtcgtgat ttccacgacg ggtgccgtgt tatcgcgcac
    13561 ataggtgcca gacaattgcg tgttattacc agcggcatcg gtttcgctca ggttaataac
    13621 tttgcttccg tcattggcac taaaggtcaa ttgagcctga taagtatttg ctgcacagac
    13681 cagggaactt gacgacgaaa gtccagctcc cgagaaggtc acggtgagtc ctgtctcaca
    13741 ggctcccgtt agtaaaactc cattttgcgc aatggtccca tttaatggag aagttatagt
    13801 caggactggg gccagagtat cgcgaagata acttttcgaa gtgctggcga cattccctgc
    13861 agcatctgtt tgctcagcct ttacaatctt ggctccatca cccgctgaaa ctgttacgct
    13921 ggcagaataa gttcctgcgt tacaaggaac tgtcgtggca gtactgaggc ctggcccgct
    13981 caccgtaacc ggaagcccac cttcacaagt gccttgtact gtaaagttgg cagctaaata
    14041 gctattcgcc gcgggcgaag aaatggtaat cgtaggtgcc gtcaaatcac tcatgaagtt
    14101 tcttgaatct gagcccacgt ttcctgacga atccgtttgt gcagctgtta tcagtttaac
    14161 accactgcct gcggtaaaaa aaatggaagc actaaagtcg ccttgagctg agcagggaac
    14221 caccaccgcg gtggcaacac cacttcctgc taaagtgaca ttcagacccg cctcgcaagt
    14281 tcccgacact gtcaaatcac cttgccctgg gctattggcg gctggagatg taatggccac
    14341 cttggggggt gtcgagtccc tcagataatt ggtcttagct gtactctcat tgccggctcc
    14401 atcaacttca ttggccgcga taattttgct gccatctccc gcactcaggt tgagatttac
    14461 actgtaggct ccagcattcg agcaaacagt tgaggcctga ctagcaaggc ccgcacccga
    14521 aaaatgaatg gttagtcctg actcacaaga gccactcaaa acgaagtttg caggaacaga
    14581 ggtccctgcc accggagaaa tgattgtcaa gcgaggagcc agtgtatccc gtaagaagtt
    14641 tctttcagct gaactggtat tcccacccag atcaatttgt gtcgctgtca cttgtttaac
    14701 gccatccaaa ggagaggccg cgacagaggc ggtgaaagtt ccattcgggc aagaggcgga
    14761 agtcgcaggt aaaattccag atcctgaaat ctgtacggtc aaacccattt cgcaagttcc
    14821 taccacttca aagttagcac ctacatagct gttcgctccc gggcttgtga tggctagaac
    14881 tggcggcagg ctatcgactg tgaaggattg ctgtacttgc cctttattgc cagcaccatc
    14941 ggtctgagtt gcgatcaaac tcttggagcc ctctccagaa gtaatagcgc tggttaaggt
    15001 ccacgcgccg gagctgcaag cgattgactt gggcccacgg agcgcggttc ctgataactc
    15061 caccggcaga cctgtttcgc aggctccaga gaaagtgctc gtcggagcca aataggcatt
    15121 cgccagtggc aaagcaatcg ttaccaccgg agccactgta tctttgatcg tattttttga
    15181 atcacttccc agattacccg cagtgtcgac ttggaaaacc ctgagaagtg ctccaccatc
    15241 cgcaactggc aacgagatgt tcgccgtgaa gcttccgttg gcgcaactga cagcaggagc
    15301 ggtggtcgca tgcaactctc caatctccaa aggcagtcca gcttcgcagg tgcccgatac
    15361 ttccaagctt gcaagattgg tgaccagcgg aattgcagaa gtgatttgaa ctttcggcgg
    15421 aaccgtatcc acactgaggg tgctggaagc taagccttga ttgcccgcac gatccacttg
    15481 tcccccgacg atatctttta tgccgtcacc tgggcggact ggaatcactg tggagaaggt
    15541 tccgtttttg caatccgctg acacgggagt ggacagaccc gggccccaca gcatgactgg
    15601 caaaccagat tcacaggtac ccgatacagg gaccgaagtg gcattcgtgc tctgcgctga
    15661 gttctcactt gaaatattga ctgcgggcgg aattgtgtct ttcataaaac acatgcgatc
    15721 ggcgatttcg ctgccatcag cggctgtttt ctgagagacc aaaaggtctt ttgttccgtc
    15781 tccatcagag agttgaagtt cggttgcgaa tacattttta atgcaatcca cagtcgtggt
    15841 cttacccgag atttcttgga ttaaaactgg aattccactt acacaactgc ctgtcacatt
    15901 cacttgaccc gtggccaccg tgtccgccgt aggatacaaa atcacgggac ctggattcac
    15961 accaaacttt gcagaagagg taggaacttg acaggaaacc ggatccactg aaaaaaataa
    16021 tttagtatca gtgcaagccg ttgctaggac taatgctccg caataagtta gcagcttctt
    16081 taatgtatta aattgcgaca tgacccactc tccgatttcc ctatctgagc ggatctattg
    16141 caagttcgag tccaataaaa caatccagtg tcaacagagc aggtatagga ttctattgat
    16201 gaatctcatt atgaaatctt cgattttttg cgtacacgtt tgtactcaca aaaaaggtag
    16261 tcagtttcta gaaaataaaa aataaacact attttacaaa atccttgatt ttttaagcgc
    16321 atttattatt caaaaatttc actcaggact cgacctgtgg ctgtcgctgg cggaagagtt
    16381 cccattatga aagagagtgt cggcgcaaga tctatcactt ttgccgctga cgaatagacc
    16441 ccgcttttaa tctcaggccc tagtaaaatt aatggaacag tgctgtcgta ggaataacct
    16501 gtcatatgat taactacgga ttcccctttg ctaatgtaaa aaggtcttgg aattaaaata
    16561 acatctccat ttttaccggc aatatactgt cgaaccaatt gctgacctag ctcccctact
    16621 ggcaaaagcc ctttggcgat ctctgaacgt gtcgccactt ggaatacgcc agggaacgtt
    16681 tgcatgacat tcttgatttc agcctccacc aaatcggaac ttacttttct ttcttggagt
    16741 gcctctggat ttaaataaaa attaaaatta attgccgcgg caatccattc tttcgtcggc
    16801 tcaccaaact tgtgattaag tctttcatag atcttcttat acatagcaag ataatcaata
    16861 taaccggact ccaactttgc gtttgtcgca tattccacac tgggagcgac gccatggtct
    16921 cctgtcaggg cgatgaccac ttcatccaaa gaacccaaat gtttctttag tttactcagg
    16981 agttgggaga tctctttgtc ttccgctaaa gtcatcgttt ttagctccgc tgaatttggc
    17041 ccataaatat gcccagccat atcatgagaa gaaaaactga cggcgagaat gtctgttctg
    17101 ccattcttac ccaaattctc gctctttaaa gcggcctcag ctatttctcg agtgatctgc
    17161 gcgccataca ccgtgattaa acgctttttg ttttctgcct tatcatcctt ttttaaaatt
    17221 ccagccttgc gcaaattttc attgatatcc ttcacccaag ttggaatctc tttttgatag
    17281 tacttgctgg tagtccagtt gaaatatttc tcatccatcc acagagcgat atcactgcga
    17341 tggcctccta gcatcaccgc tgctcggtcc tttaatgcca cggatacgac ttttgacgct
    17401 tcggaaatat ttttaagctc atcgctaaaa gtactggttt taagatgacg tggagaaact
    17461 ccgaattcgg cgtcttccgc gcagtatatt ttctttcggg ttcccacctc ataccaatca
    17521 ttcaaagaga ttccagtgaa gtaaggatga gcaccagttg aaatcatcgc atgtcccggg
    17581 catgtcattg actgaaagac gtcgtattgg gcaaaaggaa agtaagctcc ttttctcata
    17641 agatagttaa aaccaccgac ttcgcctttg gcctcgggca aaacaaattc tttctgatat
    17701 ttcgtgagat agtccgcacg aaattgatca atcactaaaa ccacgacaag ctttggcttc
    17761 tttttgtatt tatcgagagt cagggcttgc gactgagaga aagaaaaacc gaggaagaga
    17821 gaaaataaaa taaatatttt catcttggaa aaatcctccg ttactggcac ttaaatttca
    17881 tattcacaga gtaacccttg ccgagagctt cagaagcaat gcctccgcca aggcccagaa
    17941 ttcccccggc ggatcgttca ttctttccgc caaagacata gccggcgcca cccacggctt
    18001 gagccacctt cgctgtcgtt ttagctgtct tataatcgct ttcactcata gaaccttcat
    18061 atttactttc ttcatttaag aaaaccgcca ttttctcata ttttttacag aaatcatttg
    18121 cttgctctat agcttcgcga ctgcccgcct ctttatcctc tgaagtaatt ctaacgctat
    18181 ggataccatc ggaacctggt cgaacgtctc gatgatgcgc acaagctccc gagagcaata
    18241 gagctaagat catagcttta cgagcacaat ttaaaaaaaa tcgtttaaaa cctgaatgtt
    18301 tatgactttg ccgaaagttt tccataatac ctcttacttt ataagaataa tagtatttgg
    18361 attattttaa taaaactcaa aaatgtccac ttaaaatgat tcatttaagt aattcgtggt
    18421 ttgaagcgtg aatggatctt gggaggttca tttcttaggg atgattttta ttattactta
    18481 taataaacta atacccagag aatcatcaag ggctactcac tttgaaagct atttgggatc
    18541 gcgagcggta ttgagagaaa tttcccgaca tccaataatc agcgcatcat tttttattta
    18601 aacggaaata accttgacag cggttttata aaaaatcttt tttcattatt tccatagtgc
    18661 aagtctcgtg aaagtggctc tagcaatgac tgagaggccg ctgaaaggct cttctttttg
    18721 aatacaagag cgggccccct tagcttaaaa tcaaaccctc gtcttggccc ttttaagaga
    18781 gaaaaatgag atttaaaaac tcgtgataga gtgcccttgg atctcaaggc aaataaataa
    18841 aacccccgcg cttaagcaat gccgctctgg tgcagggctt gatccaacgc acggaaaaag
    18901 aggccccttt aaaacaagaa atgcttgaaa gtgttaaggg aaaaaccacc aaacaagcca
    18961 aagcttcttt aacggctcag catccagtgg cgagcttacc gttaaatcaa gaaaaaccct
    19021 tgagcgccac ccacacgcaa attttactca ctctagatca agaaaccctg aagctcttta
    19081 aagaagccaa gaatttactc agccacagta ttgccgatgg aaacttgagt gaagtcttaa
    19141 aagccatctc aaacattgca atcgaacatt tagctaaaaa gaaaggccgc actccaccta
    19201 aaatcaaaac cgaaactcct ttaacttctt ctaaaggaaa aacaaaaaaa gcaatagctg
    19261 aagcagaagt aggagaaaaa gaacgaggag ttgaaaaaga tgccgtcgaa acaaaagaga
    19321 aagtagcgga gtccccgaca tcggagaaac agagtgtcac ccataacagc cgatacctct
    19381 ctcaggaaac caagcgcatc gtctttgctc gggcgggagg gtgttgtgaa tttgtaggtt
    19441 ctgagggcca tcgttgtacc agcacttttc agctcgaaat tgatcacctt gagccttgga
    19501 gccaaggcgg tagcaacagg gctgagaact tatcgctgaa atgcaaatct cataatttct
    19561 accgcgccaa agagacccac ggatttttct acaaggctca gaagtaagta aaattccaag
    19621 gaactcaagc aaccccagta aaccccagaa acccccgaga agtttctttt tccctcgaag
    19681 ttctggccac gatggccaga actcagcatt cccagggatg gaggcacttc gcgcaacccc
    19741 tcgacagctg ggcgaagacg agaacagctc ctattcctat tcttcctact cccaatcgca
    19801 ttttccctct tgtattccat aactcccgcc gatgcttaac ccgcaaattt caaacgagtc
    19861 cttaaaataa aaaagcggct tgtgttccac agccgctttt ttttgcttta ttttttcaat
    19921 tcatctttaa tattcttagt tattcagggc gcccgcattg atccagttcg caatgttttg
    19981 aagctcctgt gaggtcaaag tagtcgctga agggtgacca cttttaactt tggtataaag
    20041 cttactggaa gcggcatttc ctgccacaac ggccttcatc acacctgcgt atgtagagaa
    20101 ggtatatcca ccggaaccac catgacaacc cacgcactta ggttgaatca ttgtattgaa
    20161 aatgttagta aaggtcgcgc tgctgttaac tggcgttgga gccggtgttg gcttcggtgt
    20221 cggtgctggc gttggagctg gcgtcgaagc cggtgttggc cttggtgttg gagctggcgt
    20281 cggagccggt gttggcttcg gtgtcggtgc tggcgttgga gctggcgtcg aagtcggtgt
    20341 tggccttggt gttggagctg gcgttggagc cggagttggc ttcggtgtcg gtgctggcgt
    20401 tggagctggc gttggagctg gagtcgaagc cggtgttggc cttggtgttg gtgctggtgt
    20461 tggtgctggc gttggagctg gcgttggagc tggcgttgga gccggagttg gcttcggtgt
    20521 cggtgctggc gttggcaccg gagccggtac tacaccacta aaacaagctc tcagcttctt
    20581 tcgcaatgca gacaactgct cttttatagc taagcactca gtcgagcctt cttcccttga
    20641 gcaaatatct cttgcctgcg ctcgcaaagc agacctttgg gtttcgatat cctctacgca
    20701 aactcgaatg ctgtcacgac ttggccttga agaagaaacc ctagtacggg attcgtgtct
    20761 atccagcgat ttggtttttg cgtaagtgct agttcctgtg gtcagaaaaa aaacgagagg
    20821 caatgtgagc ttaaaattta ctttcatttt ggtttccttt gtttagactt ctttttatca
    20881 tccttaactc tgcgtgcgat tcatcgatct ctatcttcaa ggagatttac atgcggaaac
    20941 gatcaagact cccgttagac tttgccagat tagcacaggc aacgattgat ccgaaaaaaa
    21001 taaattcaaa atctcactct aaagcagacg aagaatttaa ttatttattt aaaataacaa
    21061 atcttcatct atgtcttgtc catggtgtta gtaaagtatt gaatgtaaaa aatgtcgaca
    21121 atttcaaaat ttgcttttaa acgcagactt ttcgttctct tagtctcgct gaaaatattt
    21181 acaaagcaaa gagaaccaaa atatcgtcgc accatcctga gacaggacca gtcatttcaa
    21241 gggtttagga ttggcctttt tattgaattg tgtagttatt aggttctagc ttagaagtgc
    21301 taaacacgct ggggattgtt gatgagggaa gatagaatta ttgtaatgta tttaattttt
    21361 acacttcttt ttggaggcgg catcctccta aaaaaaataa ttcatcgaat tccaatagca
    21421 aattcacaat cggtcacgaa agaaaaagat ccctataaat attctcatca aaatcccaat
    21481 caaacaacat cacagccaat ccctcgagtg cctccccctc agccaacgga gacacgccaa
    21541 atcgcttcga tccccaaaaa tagaccacaa agtcatccct ccgagccgct gcgaaaattt
    21601 tcacaacaaa ccctggctgc ctcacggtac tcaaacgact atgatgaggg ccacgatctt
    21661 tcccaaaaac tcgatagtct tgattatcag gaacgaaagg aaatggattt tttaaaaatg
    21721 aaagtaggcc tgagtgaatt tgaaattcaa actctagaaa agaaaagaca tgactaccag
    21781 gctcagttgc aacaatttag cactcaagag cagcaaggct ccaaagaagc cggcatcagt
    21841 aagcaatccc ttattaaagc ccacatccaa tggatggcca attatttagg gcccgagaat
    21901 tattatgaat tcctcaactt atcgacggaa taaaaaagtc gaccttgatt gggtcatcca
    21961 ttgacgcaaa ggtttttcaa aataatgatg catgagcgag gccacaagaa acaaaaccat
    22021 caaagtcacc gttaacgcca caaagccgct tatcgctgac tgaagctctc caaatttata
    22081 acgccagagc ttaagcagag tgagtcccac aggaacatgc aacatataaa gagagtaaga
    22141 gatttctccc agataattgc tcactcgatt tcctaaaatg aatgtccata aacgcgaacc
    22201 caagactacg gaaaaaataa gcactgtagc taaaggaata atgacggggt ccggaagagg
    22261 gctctctaag aagagcccta aagagattgc agctatgact gtcaagacag tgcctaatga
    22321 ttttttaatg gaaactgtta acattggccc aagcatttgc cccaagcgcc aaaggctgag
    22381 tccgatccaa aattcaatga gacagcgata ccatccccag tcccaggtca tatccattga
    22441 gccttcttta agtgaatagg ccagccaaat aagtgcagcc ataggcaaaa tgactgctgc
    22501 agctattcca gacaacgagc gagagacttt gcgattcagt aaaagcaata gcggaaagga
    22561 aagataagcg gcccactcgg cgctgatact ccaagaagga taattccagt ctggtgtata
    22621 aaactccccc caggaatggg tcaaggttag atgagccagg atcgatcgcc cccactgctc
    22681 ccagggaacc gtcggcactg aaaaaagagc aagacccatc attaaaagaa gcgagaacag
    22741 atgaagagga taaatgcgcg cgaggcgtct ccaaataaag gatttaaaaa aatcaaacgg
    22801 atttcgcccc tcccaagagg cacaagtgag ggccatcaca tagccactca aaataaaaaa
    22861 aatatccaca gctaggtagc ccttttggat aaatcgacta ggaaacaata aaaaataaaa
    22921 atcaggggaa aaattaaaac catattgtaa atgaaaaaag gtcacccaca gggccgccca
    22981 cccccgcaaa cccgtgagtt cacgcacgta tggcttttcc atggtcaaca tttagacggc
    23041 atcttttcaa taagctgcgt aagataatcg acacggccat ttaggcaagg aaccaggtgg
    23101 tagctctccc cacccgcctg cttaaaagtc tcttcaccct ccaggccaat ttcttccaaa
    23161 gtttctagac aatccgaaac aaagctgggg caaagaactg ccaggctctt tactccgcgg
    23221 cctggcaggg cttttaattc atctgacgta taaggtttga tccaagtctc aactcccacg
    23281 cgggattgaa aagaaaaacc ccattgctct ttactcagcc ccaaggcctc cgctaaaagt
    23341 gcggaatttt taatacattg ctcacgataa gcattctcag gatccatctt gcgattctga
    23401 ctttcaggca aaccatgata ggtcattaaa tagtaatcca acttttttcc ctgaagattt
    23461 ttctgggttt cttcagcaac agcgtggata aactcttttt gatcccaaaa aggaagaacc
    23521 ttttgcagac gaaaaaaata ctgatgttgt tttataagtt catcgacttt tttgaaactc
    23581 gactcagttg tggcgcgaca gtactgcggg tataaaggaa ggaaataaac ttcttcaaca
    23641 tccagctctt taaatttttg cagagcctgc tgcagagagg gctgtccgta gcgcattccc
    23701 aattccacca cgtactcgtt ccccagggcc ttctgtaaat tttctttttg cagcaaagag
    23761 ttcaccatca aaggagatcc ctgtgaagtc caaattgctt tatacttggc tgcggacgcg
    23821 tcggctcttc ttggtacaat accgacatga accagcagat atcgaaataa aaaaggaatc
    23881 ggcaaaattt cctcatccat cagaaattcc cgcaagtaaa cagcgacatc tttgggctga
    23941 tatgaagcgg gagtcccgat attcgacaat aaaacgcctt taagtaattt catggaccag
    24001 ttccttgctt cgactcaaaa ttttgctaag accaatcccc cccatataat tgccattcag
    24061 atagagattt tttggaagct ttaattcttc gagaattttt ttgtgctcta gtgtgtaatg
    24121 aggcaacgcc tgaggtgaag gattcattcg gtaatcaaaa tatctgtcct cgacccccag
    24181 aatccttttt ctttccatct gaatgcgagc caatatttcg tcaggggact tagcgaccat
    24241 ctccggagaa tgtgctccgc caaaaatcca ggtttcgctg taagccggtc cgcgattttc
    24301 aaaaatatag gcgtttgaca gaacccccaa aacacgaaaa ttttggtctt caggaaagag
    24361 cgctccgaaa ccgacgattt tagcttgagg ttccttaaag aaaagtgtgg cactcaaaac
    24421 cggtaaaact tcaatccgcg acaaacttga agccagctcc gcagagactt tccttacgac
    24481 ccggggagct acagccgcag aaacgcaaat cacatgggga atcgtcaagt cttcaggctc
    24541 atagactgaa ttcaaataaa tccgcacccc ctcttggcgc agcttccgct ctaaggctgc
    24601 gatgaactca cccattccat tttgaaaact aatcgttcct cgatagcctc ttttcgtctt
    24661 tggatttttt cttttaaagt acgggccgat gatcaactca gcgctcattt gctcgacatt
    24721 gccagcgtaa atcccctgca aaaccggagc taaaagatat tttgtcagtc cacgggtaaa
    24781 attgcgcaaa ccccaagccc acatggtttc ttgcgcttga ggtttcaagg aggctctcct
    24841 aaaaatcatt ctaggaataa ctttaaagaa aagtttcaaa ctctcaaaaa tcgtcagtgg
    24901 ccagcgtttg aggcgcttac gaaaaataaa tctttttttg gcccccttca agggtcgtat
    24961 gtattcaagt ttaagatcag caaggagttc cgctaagtcc tcattgaata tgaagccatt
    25021 cgccgcattt tcgaccaaac cagaagaggt cctctcggtt tgcagaagcc cccccagttt
    25081 tggactggcc tcgtgcagat ccacagaaaa accctgacga cttaaatagt aagccgtcac
    25141 cagacccgaa aaacctccac ctaccacaac gacttttttc atatagactt cgagaatact
    25201 tgagtttgag gctgcttgag gcgcagacgt tcatcaaaaa atacacaggc gttgcgcaag
    25261 aaaggacgtc ccttttcggt gatcttgacc tcttgctcat tcacttcaac aagcccatct
    25321 ttggccattt ccgcaagaaa aaccctggca gcgttagctt gctgagcatt cttgaaagaa
    25381 acgcgaagct gggtcataag tttcaaaatt tgctcacgat gctcccgatc ctcatcggtg
    25441 tgaacatgac cacgatgagt ggggatttcg ttcttttcca gacgctcttg ataaagattc
    25501 aaaagttttt cattttgatg gaagctgtaa ggggtttccg aaatcgaact cacacccaaa
    25561 cctaacatca cttcgctctt ttgatcagtg tagcccatga aatttcgatg aagtttttta
    25621 ttttgcgctg ccaggcttaa ggcctcggaa ggcaaagcaa agtgatccat gccgatttcc
    25681 acataacctg cattcataag aatctcacgg gcaatttcgt aaagcttgcg cttttcagcg
    25741 ccgctgggaa gatcctcatc tttaaaaagc cgttgtgccg gtttaatcca agggaccaga
    25801 gcaaagctat ataaagcgat acggtctggt ttgagtgcca ccgtcaacct tgctgtctcg
    25861 gcaaaagatt ctggcgtctg tttcgcaagt ccataaatca aatcataatt cacggaatga
    25921 aaccccagat cgcgagcttg ttgggtcaaa ttcgcggtga tttcataagg ctgaaagcga
    25981 ttgaccagct tttgtacttc gggattgaag tcttgaaccc ccatactgac gcgatcaa
//
LOCUS       DATBTE010000275         2711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_172164, whole genome shotgun sequence.
ACCESSION   DATBTE010000275 DATBTE010000000
VERSION     DATBTE010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172164"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..306)
                     /locus_tag="VIG33_10170"
     CDS             complement(13..306)
                     /locus_tag="VIG33_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271241.1"
                     /translation="MFVAFSKINPQMLITQENNIVSQEFAQNSGSHQQDRENVNSVTI
                     EDEEDDVEGDDFIVISTLPSLKDTPRSRQYDFRNYNTLDQPKAVNLPPPKLLS"
     gene            816..1697
                     /locus_tag="VIG33_10175"
     CDS             816..1697
                     /locus_tag="VIG33_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8271242.1"
                     /translation="MPRPQIQNEPKPPFPKERQKAPGLESKMSVRPRYKAPRYKPSGK
                     LVGKRALITGGDSGIGRAVALIYVREGASVAITFLPEERTDAERTLLEIEEIGGKCIL
                     IPGDLTNEKFCKKAVERTVKELGGIDILVSNAAHQMRKKDISEITTEEFDLTFKTNIY
                     AYFHLAKAAIPHMPPGSAIIATSSETATRAPGNLLDYASTKGAINTFTKSLAQMLIDR
                     GIRVNAVAPGPVWTPLNPSDAGASKNKISEFGANQPIKRPAQPEEIAPAYVFLASDAD
                     SSYVSGIILPEMGAEPE"
     gene            complement(1723..2460)
                     /locus_tag="VIG33_10180"
     CDS             complement(1723..2460)
                     /locus_tag="VIG33_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271243.1"
                     /translation="MKFISVILASFFFCFFVCSCSLLGFGSKRAPEVVPSCFSDSENQ
                     ASYCKTQRVNEDRRIVTTRCIGSQNQEAKSNLRGKCVEKICSEGSNTDCQVKGEFAVL
                     EQYAELTTAKLFAADEEGQPKKEAAVKDKDKEKEKAKTKVAANAPILPAEAVSASKKT
                     EFKEEEAEKSSMKIVLKPIKKSRAPASIKESEKSTKKFCIAKTETTAPEALRGKCATR
                     TCFNGKCTYHGRKAVFEWGSHAEAAPK"
BASE COUNT          766 a          586 c          549 g          810 t
ORIGIN      
        1 gcccatccta ttctagctaa ggagttttgg aggagggaga ttcactgctt taggttgatc
       61 taaggtatta taattcctga aatcatattg tcttgaacgt ggagtatctt tcaaagaagg
      121 taaggtcgat attacgataa aatcatcgcc ttcaacgtcg tcctcttcat cctcgatagt
      181 gacactgtta acattttctc gatcctgctg atgacttccg gagttttgtg caaattcttg
      241 gcttacaata ttgttttcct gagtaatgag catctgtgga ttgattttgc tgaaggccac
      301 gaacaaatga cctccgcata tcaataagat atacgccaaa aactggaaga ccagacagat
      361 gtaaaaaacc ttctttttag tcattctgga gaaatttatc accatcgaaa aaaattatca
      421 atgcactttc tccgattaaa tagaaactct tgttgaaatc cagcctcaaa aaaacctgat
      481 agttcacaca gggagggaat atccgttgag agcatgttga aatcacttag aataatctat
      541 cgttatatca atggaggcgc tgaaaggaaa aaagttcaaa aggagatgat ctttgaaaga
      601 tttggataaa agtatacatt ttgaatcttt gagcgaggag gtctcccata ttgaaagtga
      661 aactcgcatg atcttaggct gtgtttttga tttttttctg atcggattta tcatcacgca
      721 gagtacgcct ctaagcgcaa cgatcacatt tttattattt ttgctgtttg taggggtctg
      781 gtacatttac ccctggtcgg caataaggag attccatgcc tcgcccccaa attcaaaatg
      841 aacccaaacc tccctttcca aaagaacggc aaaaagcgcc gggcttggaa tccaagatga
      901 gtgttcgccc tcgctacaaa gctccccgct acaagccctc tggaaaactg gtgggtaaaa
      961 gggcgctcat taccggaggc gattcaggaa tcgggcgtgc ggtggccctc atctatgtac
     1021 gtgaaggcgc ctcggtggcg attacttttc ttcctgagga acggacagat gccgaaagaa
     1081 ctcttttgga aattgaagag attgggggga aatgcatttt aattcccggc gacttaacca
     1141 atgaaaaatt ctgtaaaaag gccgttgaaa gaaccgtaaa agaattgggt ggaattgata
     1201 tcttagtcag caatgccgct catcaaatgc gtaaaaaaga tatttctgaa atcactacgg
     1261 aagagttcga tcttacattc aaaaccaata tctatgctta ttttcactta gcgaaagccg
     1321 ccattcccca tatgccaccg ggctccgcaa ttatagccac gagttcagaa accgccactc
     1381 gagctccagg aaatctttta gattacgcct ctacgaaagg tgctattaat acctttacta
     1441 agtctcttgc gcagatgttg attgaccggg gtattcgagt caatgcagta gcccctggac
     1501 cagtatggac tccacttaac ccttcagacg cgggagccag caagaataaa ataagtgagt
     1561 ttggagccaa tcagcccatc aaaagaccag ctcaacccga agagattgca cccgcctatg
     1621 tgtttttggc ttcggatgcg gattctagtt acgtgagcgg aatcattctt ccagaaatgg
     1681 gagcagaacc ggaataagct gtgactccta gacctcagga cattacttag gagcagcttc
     1741 cgcatgagag ccccattcaa agacggcctt tctcccatga taagtgcact ttccgttgaa
     1801 gcaagttcgg gtagcacatt tgccacgaag cgcttccggt gcggtagttt cagttttcgc
     1861 tatacagaat tttttagtgc ttttctctga ctctttaata ctggcgggag ctcgcgattt
     1921 ttttatcggt ttcaatacta ttttcattga acttttttca gcttcctctt ccttaaattc
     1981 tgttttttta gaagctgaaa ctgcttcggc tgggagtata ggagcattgg cagccacttt
     2041 agtctttgct ttctcttttt ccttatcctt atcttttacg gccgcttctt tcttcggctg
     2101 cccctcctca tcggccgcaa agagctttgc tgttgttaat tcggcatatt gctcaaggac
     2161 tgcaaattca cctttaacct gacaatcagt attggaacct tcagaacaaa tcttttccac
     2221 acatttacca cgaagatttg atttagcctc ttgattctgt gaaccaatac acctggtcgt
     2281 aacaatccga cggtcctcat tcaccctttg agttttgcaa taacttgcct gattctcgct
     2341 atcactgaaa caactaggta caacttctgg tgctcgtttt gaaccgaaac ccaataatga
     2401 acaactgcac acaaagaagc aaaaaaagaa acttgctaag ataacactga tgaatttcaa
     2461 atgaagactc ctgttgtaga cattcattat tgaatgagaa agcgtttatc ttgtaaagct
     2521 gaattttatt tatttattat aagtttctct catttggaac cgtaaagtaa aatgggttcg
     2581 ttaaaaatat ttgtgggcta actctgggac cggaagtttc ccaagcttat gataaagcat
     2641 gatttttagg atttctgcgg tcttaaatcc aaaagatttt ctgatcacca cctttgcttt
     2701 attattttgt c
//
LOCUS       DATBTE010000276         2686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1128671, whole genome shotgun sequence.
ACCESSION   DATBTE010000276 DATBTE010000000
VERSION     DATBTE010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128671"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VIG33_10185"
     CDS             complement(<1..418)
                     /locus_tag="VIG33_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271244.1"
                     /translation="MSQFRLVILDNAKEQLDNPIAKKILSDVVFVKQKNFLRTEPNYV
                     VTDKHDMIGTHYLIYDTSDFLNPKLVFAIRTTFLKRALAHRIDTPLMSLISRLEEPLR
                     EAFNNFHKRHKEIVDCNAWFVDPQYSKKNSGLNLSDL"
     gene            559..1428
                     /locus_tag="VIG33_10190"
     CDS             559..1428
                     /locus_tag="VIG33_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEY8271245.1"
                     /translation="MPTHELDSSQENYLERTKRNHFWTCGIEGQLKLKKIKVGIAGLG
                     GMGSNIAEILVRLGVGHLRIGDPDIIEISNLNRQVIANLSTIGMSKLDASIKELINID
                     PTLTIDAYPEGISAANAESFVEGLDIVINEIDVLHMDVQIHLLNAAKKKNIPVYTTLV
                     VGVGIHLYKYDPESTFTAHDFLGGILKNSSLDTLLETLGQPLPSYLQGQNLEDFKEEV
                     TTKGGIPIFGASTYLGQSMLVIRALADQGYLRTTKNLPPTPALPEFLILDPLTLELRT
                     AIIGKDGKAKFKT"
     gene            1403..2158
                     /locus_tag="VIG33_10195"
     CDS             1403..2158
                     /locus_tag="VIG33_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8271246.1"
                     /translation="MEKQNSKHNILFFHMGPGFHAKIEALQFSSKHPRVAFLDQPLNA
                     TFSELTDWACEVIRKYAQNHTGKVKLLGHSFGGQVIAAAMPSIGDLVSEIRFLNSAYD
                     SFDCFVNLENTLEPQNLKPLQYWKAKSLEEKMSLIFHISQLPQFSSTYWRNVEARNTY
                     EKISTSYPPVDIDSFVKAFSDYLQRPQSKRKDIWKGPTKIYYSCSDNLIKSFKVISPW
                     KESFPNAHFIEVFNIGHHGLFESPQLADDFFKD"
     gene            complement(2160..>2686)
                     /locus_tag="VIG33_10200"
     CDS             complement(2160..>2686)
                     /locus_tag="VIG33_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8271247.1"
                     /translation="LIGVIAEAETPQATREMDRFLESMKKGGAKSGMTVFVNSHFGES
                     FRVSKDPQVVALREKIMEHLLHLSPEQLENGRQIFHRQMISKNELQKITVPVLILAGD
                     EDQPSNISAYKRLSQVIPHVNYKTLQHAGYYVAIEQPQAVIQLLHEHIEKAESAFSSR
                     ISAKGKEKEKRSRH"
BASE COUNT          817 a          567 c          514 g          788 t
ORIGIN      
        1 caagatctga aagattcaat ccggaatttt ttttcgaata ttgaggatct acaaaccagg
       61 cgttgcaatc gacaatttct ttatgcctct tatggaaatt gttaaatgcc tcccgaagag
      121 gctcctctag gcgagaaatc aggctcatca aaggcgtatc tatacggtgg gctagggccc
      181 gcttcaaaaa agtagttctg attgcaaaca ccaacttagg atttaaaaaa tcactggtat
      241 catatattag ataatgcgta ccgatcatat cgtgcttatc agttacgaca taattcggct
      301 ctgttcttaa aaagtttttt tgtttcacaa aaaccacgtc ggacagaatt ttttttgcga
      361 taggattgtc taattgttct ttagcgttgt ctaaaattac caaacgaaat tgactcattt
      421 aaaacccctc aagtgtaatg aattttcggc aggcatgaaa aaaaattaag tctagttgat
      481 aaatacattt tatttaaaat gaaaaataaa gatatactct aatgcagtag ccataagcac
      541 aaagatagga agacgcatat gcccacacat gaactagact ctagccagga aaattatctt
      601 gaaagaacaa agcgaaatca tttttggacc tgcggaattg agggacagct caagcttaag
      661 aaaatcaaag taggtatcgc tggccttggc ggaatgggca gcaatattgc tgaaatactc
      721 gttagactcg gagtcggaca cttgcgtatc ggtgatccag atattataga aatcagcaat
      781 ctcaatcgtc aagtcatcgc taacctttcc acgatcggaa tgagtaaact cgatgcctcc
      841 ataaaagaat taatcaatat cgaccctact ttaaccatcg atgcttatcc ggaagggatc
      901 agcgctgcca atgctgaatc ttttgttgaa ggtctggata tagtaattaa tgaaattgat
      961 gtccttcaca tggacgtcca aattcactta ttgaacgccg ctaaaaagaa gaatatccct
     1021 gtctatacca ctcttgtagt gggcgtagga atacaccttt ataaatatga tcccgaaagt
     1081 acattcacgg cacatgactt tctgggagga atccttaaaa actcaagttt agatacattg
     1141 cttgagactt tgggtcagcc actgccctct tatctccaag ggcaaaatct tgaagatttc
     1201 aaagaagaag tcactaccaa aggcggtatc cctatttttg gtgcaagcac ctatctagga
     1261 caatccatgt tggtgatccg agcattagct gaccaaggat atttacgtac aacaaaaaat
     1321 ttacctccaa ctcccgcgct tccagaattt ttaatattgg atcccttaac ccttgaatta
     1381 agaacagcta ttataggtaa agatggaaaa gcaaaattca aaacataata tacttttctt
     1441 ccatatgggc cctggcttcc atgcgaagat agaggctctt caattttcct cgaaacaccc
     1501 gagagtggcg tttttagatc aacctctcaa tgccactttt tcggaactta ctgattgggc
     1561 ctgtgaggtc attcgaaaat atgctcaaaa tcacactgga aaagttaagc tcttaggcca
     1621 tagttttggt ggacaagtga tcgcggcggc aatgccgtca atcggtgact tggtctctga
     1681 aatacgcttc ttaaattcag cctatgattc ttttgactgc tttgtaaacc tagaaaatac
     1741 actagagcct caaaacttaa agccccttca gtattggaaa gcaaaaagtc tcgaagagaa
     1801 gatgagcctg atctttcata tatcccaact accacagttc tccagcacct actggaggaa
     1861 tgttgaagct cgcaacacct atgagaaaat cagcacctct tatccacctg tggatattga
     1921 ctcctttgtg aaagcattta gcgactatct tcagcgacct cagtctaaac gaaaagatat
     1981 ttggaaaggg ccgacgaaga tctattattc atgttcagat aatcttatta aaagctttaa
     2041 agtcatttct ccctggaaag agagctttcc aaacgctcat tttattgaag tttttaatat
     2101 tggccatcat ggactttttg aatcacccca attagcagat gattttttta aagactaaac
     2161 taatgccgtg accttttctc tttttcttta ccttttgcac tgattcgtga gctaaaagca
     2221 ctctccgctt tttcaatatg ctcgtgcaga agttgaatca ccgcttgggg ttgttcaatg
     2281 gctacgtagt agccagcatg ttgcaaagtc ttgtaattca catggggaat cacctgactt
     2341 agacgtttat aggcagaaat attgctaggc tggtcttcat caccagccag gataagcacg
     2401 gggacagtga ttttttgaag ttcattcttt gagatcattt gacgatgaaa tatttgtctg
     2461 ccattttcaa gttgctcagg ggataaatgt aaaagatgtt ccattatttt ttcccggagc
     2521 gccaccactt gcggatcctt gcttacgcga aaactttctc caaaatgact atttacaaaa
     2581 acagtcatac ctgatttcgc tccgcctttt ttcatggact ccaagaatcg gtccatttct
     2641 ctggtggcct gaggcgtctc cgcctccgca atgacgccga taaggg
//
LOCUS       DATBTE010000277        11591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_378615, whole genome shotgun sequence.
ACCESSION   DATBTE010000277 DATBTE010000000
VERSION     DATBTE010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11591
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378615"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..966)
                     /locus_tag="VIG33_10205"
     CDS             complement(25..966)
                     /locus_tag="VIG33_10205"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEY8271248.1"
                     /translation="MSYRRVTSEDRLRIKDGLDAGLSNSSIADKLGFHKSTIGREILR
                     NKGGRGYRPKQAHLLAVAREESKHGPYKMNPVIMAKIIERLQARWSPEQIYNRLRMEG
                     EDIVSTETIYKFILEDRKNGGELWKYLRRSGRSRKKRFPSEDRRGKIQNTRSISKRPK
                     KINKRKKLGHWERDLMIGKDHQSAVLALVDRKSRFNKFRKLNGKHAKKVTKETIIALK
                     NLPKKSMTNDCGKEFADHQSYEKKTKVKVYFCDPYSSYQRGTNENRIGILRQYLPKKS
                     DLRKVTNKQLETIEFEINNRPMKILDWKTPYEVMMEK"
     gene            1228..2469
                     /locus_tag="VIG33_10210"
     CDS             1228..2469
                     /locus_tag="VIG33_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271249.1"
                     /translation="MQQQLAHFSEQLKIYFNTPKMLFTSWTEAFAELNRHVSSKSCLI
                     FLDEISWMSSGADTFSSELKASWDMLFKKKNKLVFVIAGSVSSWIEENILQNSNFLGR
                     VTLNLRLDELPLSDFGKFWRSRGSTISTFNLVKTGTVFGGVPLYLELQNPQESVEQNL
                     NRLCFKKSGPLVEEFDKIFSDIFSRRGPIYKRIVGALILRPMTHSEIAKALGTEKSGA
                     LSKYLADLVQSGFLSADTTYDFKGAPTKLVQYRVCDNYTRFALKYIEPNKKKILEGSF
                     HFGSLEGLPGWSSIFGLQFENLILKNKNLILNRLQVDQSKVLACSPFVQRKTNKNKGG
                     CQIDLLIVLKNYHIYLCEIKLQAKISNQVIEDIKQKMAVLKRPKIYTLQPVLIYGGEL
                     SHKVKSSEDWLRLISIEDLFE"
     gene            complement(2687..7633)
                     /locus_tag="VIG33_10215"
     CDS             complement(2687..7633)
                     /locus_tag="VIG33_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271250.1"
                     /translation="MSPNSFLVVILSLALFGTGCTKSREAELPANMQASIFAITEFGD
                     IQTENSQFSMATDDTLSTLSLGNSSKATAEKGVVALTEVNVPGRLKYMFSGLTITGLA
                     AHNYPVTFSVDRQFVTAYKVVTNPMELTILEKQLAQVQEEVLLQKQLQKNKDKAKSKE
                     LLASLQKARTKKLNLLSQQQPSATLLVPIFKFKVLKFGVIQKEKNKLKEDTSTLELLE
                     TDWTQATHIQIGTTIENRLAIGLDPAQAGDLDRTFVMDRINNKIMTAETLRNEYQIPL
                     NLDNKARVLTLLDTSVLHVFEITQLDKVKDTLSDAQLRQLQSGVGSNVRACPADVVKA
                     LPADAQKECLLILRYDVDVTYVRPEQGAVDYDGNLDSKVDFKPVLASQNIGLVQVPKD
                     VIPKTVEANNTLDPRSTIRIIDIKDKEFFFKRTLEDASYSAATSGLAPGMAGAITIVK
                     FDLQEDRVVIRRADKIINYKTTSNDSDYEELMSLPVKYVKLETKDASGAAYAIPHLVP
                     ATRKDAQYINLDWTANSIPQENSPYAAVYEGCTLGIADTQVADVDMRLDKGVLNFSQK
                     YSLALKPECSADFSPANGYNGTPSYQYSARVIERVSFKVNDGSTDKPFVTPVPFKVQN
                     TLGYGVWTIGQINPDIMGQFGREGTETDLPMVQDFRNGKVLTYTLTGLPTDDAEMRKM
                     YIGIAEELAAAWNLAYKQAFKGSALERSGNYIEIQIAGENGVTAHLGDLDKNIFHFEN
                     KYASHGILGVSQVGFNPRSAIVVADSLILYTGNVKSDVANSYVLMNARAKWEKMKQGI
                     LEKARKELATEETAGQKVAKGEAPTTADPTEGKAEATKAVTHQLISKLQSSKAGYKLS
                     PKIDLKVLQRASQLALTSNDFKTALKVRQTLGNSRFAYSPPIDESGWMDRVFQTMLEK
                     PDMSISGIHGAIAKEVLASKGNKLSQTDRASLARTAKQNEIKAKMLTSLRSSPGCMKT
                     EAEVANAKFANLSFNEAFRIEALNTMVHEMGHSQGLTHNFIGSYDKANYANEDGTPSK
                     RNYSSVMDYLTPGHFSWDGLGTYDIHAVRASHLGLLEIRPEFKAKLGPKAAEVLVNDK
                     YISIQNIKAALAKNGWANFTKADAKGVLKEYKFCTDVDVGWDPMCQRHDFGSSVTEVV
                     QSIKEDWENNYISNYYSWDRNSFGMSEAYIGSAWSQHFLMQMRKYMDETFYKMIVGEG
                     TKEERKDYINASLQVYLFYNQLITTPDTNAPFLSAERFVAVPYSREELNDKGEPTGKE
                     IQDVALVEKRSLNTINRDKDRIDTRGIEFDKIAAMRLMTMKGFPSSKYESKDIEFSFL
                     DFEKYFLGMTTDTSQFINTLTSIMLDHLEPTFTPADGIVRSLAMTATITETMRQRAGI
                     YGILNLETPSIKDKDNFANLFKVGSSVGNGPSDRTVLTKLDVSPTSSARVGFWALDNA
                     TVSQYILNMAAEKNFFIQKMPEIQPAMEKLIGLQLQQELSKGVSADKKTADANITADV
                     EKARTELIGKLHALNTKGDIVSADTVKNSPKMSIESQVDGLMSFNSQVIVLSVALVSD
                     AGKIKQSLVDDVAVGAAEFADISPLAAIDQKALLASLTAAGKTLGNAKGSEAFAELDQ
                     AAGQLVTTSALESSYGIIMKNIEFLNLLTSMTNPEYSR"
     gene            complement(7650..8510)
                     /locus_tag="VIG33_10220"
     CDS             complement(7650..8510)
                     /locus_tag="VIG33_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271251.1"
                     /translation="MQTRTLSFTFFALFCGILISSTESSAQSAQNLNTSGANPTAGSK
                     PVEVKPFAISAGLSKSISLVNFQDGERQETHDLELMASYKWSLGTSLAIFTFSDDIRN
                     PGNSDVGDIALAMAFNGWNLTRFKLVPSMTVVIPESKESRINNNLETALSGKLSAAIQ
                     ERLLIPGFSFKGDLSFGRNIHRYETALDGTVLNKYASKQGLSAGYSIGIFSFAVDFSH
                     INFWTYQDTLQEFFEHTEETSLALGEHFGFTLGHTNGGSVFKENGYASNYQLIDENNS
                     LVYAKVSLQY"
     gene            8713..9477
                     /gene="rnhA"
                     /locus_tag="VIG33_10225"
     CDS             8713..9477
                     /gene="rnhA"
                     /locus_tag="VIG33_10225"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEY8271252.1"
                     /translation="MKSEFSDIVVFTDGACSGNPGPGGWGAVVVLPEGQVQELGGGNA
                     VTTNNRMEMLGVISALNFLSKTPGNIRIYTDSTYVIRGITQWVWGWRRNNWRTAEGKE
                     VSNKDLWEDLFFLVQRRKEFKDLGGISWNYVRGHRGTPGNERCDEISVAFSKGQRYSL
                     YRGSLLKYDIAILDLPELEALPEMKPKNNGVKEKAFSYLSNIGGIVYRHKDWSSCERR
                     VKGKSGAKFKKTQSASDELEVLKSWGLSPGVEIKQD"
     gene            9587..10066
                     /locus_tag="VIG33_10230"
     CDS             9587..10066
                     /locus_tag="VIG33_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271253.1"
                     /translation="MKQIMLIAVLCLSGILAHAQDGGRWGWGRPGPGRGGPGYPGRPG
                     PGYPGPGRGGGGWGGNVQCSATDSGWEEHWGGHGSCGECLQIHGNCTETCSRSYNVCQ
                     AEGIDQSGRRFTTEGRGEIRYQAEDDAMRACYYYRYQNCRIVSCNQNNETVSRRSCR"
     gene            complement(10123..11466)
                     /locus_tag="VIG33_10235"
     CDS             complement(10123..11466)
                     /locus_tag="VIG33_10235"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY8271254.1"
                     /translation="MLKILLVEDDNAFRSALKKILETKYQVIEAQDGKIARDLLSMSD
                     VNLILSDIQMPYFSGFDLLEWSQKYKPTPFILMTGFSHILDREKAKELGATDFLVKPF
                     KELELFEKIFRILGEKKEVQIGISQAPLDLDKEFCKLPIEDFIGDKETEYEIYIRISA
                     TKYIKIVHKGGILTEDKIKTFREKKVTHVFIRQEEFSKLVGFTVLVSRAVSSSGKVDK
                     EKKMRFMKYTGEMIMQQAYVAGADEGLFKNAKDFLLTSMEVLTKDEETFTLLNLLSNH
                     TDYLYSHSLGVSMFSVMIAKQLGWQSPQTLFKLSFAGLFHDIGKKEIPVELLQKPRST
                     LTQKERSLFESHSTRGREILQSLKTAPTEVIQVAYEHHEDVLGQGFPQGISKPKIHPF
                     SLIVSVADVFCDYTIRGPQNPEPQSAVAALQMMKTFKLGSLDTKAFEALSRVVVL"
BASE COUNT         3041 a         2540 c         2553 g         3457 t
ORIGIN      
        1 cctgactgca attctctagt gcaactattt ttccatcatg acctcataag gagttttcca
       61 gtcaaggatt ttcataggtc tattgttgat ttcaaactca atcgtttcca actgcttatt
      121 ggtgactttt ctgagatcgg atttctttgg caagtattga cgtaaaatgc cgatcctgtt
      181 ctcattcgtt ccgcgctgat agcttgagta ggggtcacag aaatagactt tgaccttggt
      241 tttcttttca tagctttggt ggtctgcaaa ttcttttccg cagtcatttg tcatggattt
      301 cttgggaagg tttttaagcg cgatgatagt ttccttcgtc actttttttg catgcttccc
      361 gttgagcttt ctgaatttat tgaaccggga tttacggtct acaagagcca agactgctga
      421 ctgatggtcc tttccaatca tcaaatctct ttcccaatgt cctagctttt tacgtttgtt
      481 gatcttcttg ggacgtttag aaatggatct cgtattttga atctttcctc ggcgatcttc
      541 agaggggaaa cgcttctttc tagaccggcc ggaacgcctt aggtatttcc aaagctcacc
      601 accattttta cgatcctcga ggataaactt gtagatagtt tcggtgctga ctatatcttc
      661 cccttccatt cgtaaccgat tataaatttg ttctggcgac cacctggcct gcagtctttc
      721 aatgatttta gccataatca ctggattcat cttatagggc ccatgcttgg actcttcgcg
      781 ggccactgcc agcaagtgcg cctgctttgg tcgatagcct ctgccgcctt tatttcgtaa
      841 aatttcccta ccaattgtcg acttgtgaaa acccaacttg tcagcaatag atgaatttga
      901 taagccagca tcgagaccgt cttttatccg aagacgatcc tcactggtca cccgacggta
      961 actcatttgt ttaactccat tttttcgcaa aaactgagtc tcaaatgaac cgtcaggtgg
     1021 ctaccttcaa ctcagcttgt tattgcctga gttgcactgg agaaaatttc gggggaatta
     1081 ttaagcctga aaaaccacaa aagatcctct tttgtggtat gctcagggcg tcgtcggatc
     1141 gggaaaagtt ctttaattga acaggtggga cataagtttt tccattttta tgaatttcag
     1201 gggctacacc ccagcgaagc tccgaaaatg caacagcaat tagcgcattt ttctgagcaa
     1261 cttaaaatct attttaacac tccaaaaatg ctgttcacct cgtggacaga ggccttcgct
     1321 gagttaaacc gacacgtctc ttcaaaaagt tgtctgatct ttttagatga aatttcctgg
     1381 atgagttcgg gtgctgatac tttttcatca gaacttaaag cctcttggga tatgctcttt
     1441 aaaaagaaga acaaactggt tttcgtcatt gccggatcgg tttcttcctg gatagaggaa
     1501 aatattcttc aaaactcgaa ctttttagga cgagtgactc ttaatttacg tttagatgaa
     1561 cttccacttt cagatttcgg aaagttttgg agatccagag gctcgactat ttccaccttc
     1621 aatttagtaa aaacaggtac ggttttcggt ggagtgcctc tttatctgga acttcaaaat
     1681 ccccaggaaa gtgttgaaca gaatttgaat agactgtgtt tcaagaaaag tggccctctt
     1741 gttgaagagt ttgataaaat attttctgac atcttttcga ggcgcggtcc catctataaa
     1801 cgcattgtgg gagcgcttat tctgcgtccg atgactcatt ctgagatagc caaagccctc
     1861 ggaacagaaa aaagcggcgc cctctccaaa tatcttgcgg accttgttca aagtggtttt
     1921 ttaagtgcgg acaccactta tgactttaag ggcgcaccca caaagttggt ccagtacagg
     1981 gtttgcgata actatacacg ttttgcctta aaatatattg agccaaataa gaaaaaaatt
     2041 ttagaaggca gctttcattt cggttcactt gaggggttgc cgggctggtc ttcaattttt
     2101 ggattgcagt ttgaaaactt gattctaaaa aataaaaatc tcattctcaa taggttgcag
     2161 gtagatcaaa gcaaagtgct ggcatgcagt cctttcgtgc aaagaaaaac caataagaat
     2221 aagggtggat gccaaattga tcttttaata gttcttaaaa actatcatat atatttgtgc
     2281 gaaattaagc ttcaggcaaa aatcagcaac caggttatag aagacatcaa acaaaagatg
     2341 gcagttctga aaaggcccaa aatctatact ttgcaacctg ttcttatcta cgggggcgaa
     2401 ctttcccaca aggttaaaag ttctgaggac tggctccgtt taatttccat tgaagatctc
     2461 tttgagtgag attcaactaa gttcttacca atataagatt aattccttaa gcaaaaaagg
     2521 tttgattaaa ccgcttaaac aaggggttta tctagtaagt aatgatctag gcctgattcc
     2581 agtacttcct tagaggacga aagtggcctc ggaattctcc ggaggcctct tttcttttac
     2641 gattttccac tgtctctact agcttctact tagcttaata accaacttat cggctgtact
     2701 ccgggttcgt catcgaagtc aggagattca agaactcgat gttcttcatg ataattccat
     2761 aagaagattc caacgccgag gtggttacga gttgacctgc ggcttgatca agttccgcga
     2821 aagcctcact gccttttgca ttacccaggg ttttacccgc ggcggttaaa gaggccaata
     2881 aagctttctg atcaatcgcc gcaagaggcg aaatgtccgc aaattccgcc gcaccgacgg
     2941 caacgtcatc caccagcgat tgcttgattt tacccgcatc tgaaaccagg gcgactgaca
     3001 acacaatcac ttgcgaattg aaagacatca atccatccac ttggctttcg atcgacatct
     3061 tagggctgtt cttcactgta tccgctgaga caatatcacc tttggtattg agcgcatgaa
     3121 gtttgccgat gagttcggtt ctggctttct caacatctgc ggtgatatta gcatccgccg
     3181 ttttcttatc cgcgctgact cctttagaaa gctcttgttg cagttgcagg ccaatgagct
     3241 tttccattgc aggctgaatt tcaggcattt tttggataaa gaaatttttc tcagcagcca
     3301 tgttcaagat atactgagag actgttgcat tatccaaagc ccagaagccg actcgtgccg
     3361 agctggtagg ggaaacatcc agcttagtaa gaacagttct gtcgctggga ccgttgccta
     3421 cactgcttcc caccttaaat agattcgcga aattatcttt atctttgata gagggagttt
     3481 cgaggtttag aattccatag attcccgcac gctgtctcat agtttcggtg atagtcgccg
     3541 tcattgcaag cgaacgaact atgccatcgg ccggggtgaa agtcggctca agatgatcta
     3601 gcatgatgga ggtcagagta ttgataaact gactcgtgtc tgttgtcatg cctaaaaagt
     3661 atttttcaaa gtccaaaaat gaaaattcta tatccttgga ttcatatttc gaactaggga
     3721 accctttcat agtcataagt ctcattgccg caatcttatc gaattcaatc ccccgagtat
     3781 caatccggtc tttgtcacgg ttaatagtgt ttaacgaacg tttttcaacg agagccacat
     3841 cttgaatctc ttttcccgtc ggctcaccct tgtcgtttaa ctcttctctc gagtatggga
     3901 ccgccacaaa tcgttcggca cttaaaaacg gagcattggt atccggagtg gtgattagct
     3961 gattatagaa caagtaaact tgtagggagg cgttgatata atcttttctc tcttctttgg
     4021 ttccctctcc aacgatcatt ttatagaatg tttcgtccat atactttcgc atttgcatta
     4081 aaaagtgttg tgaccaagct gaacctatat aagcttcgga catgccaaaa ctattacggt
     4141 cccaagagta gtagttggag atgtaattat tttcccagtc ctctttaata ctctggacaa
     4201 cttctgtgac agaacttccg aagtcgtgac gttggcacat gggatcccaa cccacgtcca
     4261 catcagtaca aaatttgtac tctttcaaga cacccttggc atcggctttg gtgaaattag
     4321 cccaaccatt tttagccaaa gcggctttaa tattttgaat tgaaatgtat ttgtcattga
     4381 ccaggacttc agcggccttt ggacccagtt tggctttaaa ctcgggcctt atttccaaaa
     4441 gacccaggtg ggaagccctt actgcatgga tatcataagt gcctaagcca tcccaactga
     4501 agtgtcccgg ggtcagatag tccatcactg aagagtaatt acgtttagac ggagtgccat
     4561 cttcatttgc ataattggcc ttatcgtaag aaccgataaa gttatgagtc aacccttgag
     4621 agtgacccat ctcatgaacc atggtgttca gagcctcaat gcggaacgcc tcattgaaac
     4681 tcaagttcgc aaacttggcg ttggcaacct ctgcttccgt tttcatacag cccggagaac
     4741 ttcttaaact tgtcagcatt ttggctttga tctcgttctg cttcgcggtt ctggcaaggc
     4801 tcgctctatc ggtctgagag agtttattgc ctttagaggc cagaacttct ttggcaattg
     4861 caccgtggat tccagaaatg ctcatgtccg gtttttccaa cattgtttgg aaaacccgat
     4921 ccatccagcc agattcatct atcggtggag aataagcaaa acgactgttg cctaaggttt
     4981 gtctgacttt gagagctgtt ttaaaatcat tcgaggtcag ggccaactgg ctggctctct
     5041 gcaaaacctt caagtctatt ttaggactta acttatagcc agctttggaa ctctgaagtt
     5101 tactgatcaa ttgatgagtc accgcttttg tcgcttccgc ttttccctcg gtgggatcag
     5161 cagtggtggg tgcttcgcct ttggcgactt tctggccggc cgtctcttca gtcgctaatt
     5221 ctttgcgcgc tttctcaagg atgccttgtt tcattttttc ccatttagct ctggcattca
     5281 tgaggacata gctgttggcg acatcggatt ttacattgcc agtgtaaagg atcaaagaat
     5341 cagccacgac aatggcagag cggggattaa agcccacttg cgaaacaccc aaaataccat
     5401 ggcttgcgta tttattttcg aagtggaaga tatttttgtc cagatctcct aaatgagcgg
     5461 tgacgccgtt ttcgcccgcg atttggattt caatataatt tccagagcgc tccaaggcag
     5521 agcctttgaa cgcttgcttg taagctaaat tccaggccgc cgcgagttct tcggcgattc
     5581 caatatacat cttacgcatt tccgcatcat cagtgggtaa acccgtcagt gtataagtaa
     5641 ggactttgcc gttgcggaag tcttgcacca tcggtaagtc cgtctcggtg ccttcgcgcc
     5701 cgaattgtcc cataatatcc gggttgatct gaccgatcgt ccatacgcca tagcccaaag
     5761 tattttgcac tttgaacggc acgggggtga cgaaaggctt atcagtgctg ccgtcattta
     5821 ctttaaaaga aacacgctcg ataacccggg cgctgtactg ataagaagga gttccgttat
     5881 aaccattggc tggggaaaaa tccgcagagc attcgggttt taaagccaaa gagtactttt
     5941 gcgaaaagtt taaaactcct ttgtccaaac gcatatccac atcagcgacc tgagtgtcag
     6001 cgatgccaag agtgcaacct tcatagactg cagcataggg ggagttctct tggggaattg
     6061 aattggcagt ccaatccagg ttaatatatt gggcgtcttt acgggttgcc ggaacaagat
     6121 gaggaatagc ataagccgca cccgacgcat ctttagtttc aagctttacg tattttacgg
     6181 gcagggacat aagctcttca tagtcagaat catttgaagt cgttttatag ttaatgattt
     6241 tatcagcgcg acgaatgacc acgcgatctt cttgcaggtc aaatttaaca atagtgatcg
     6301 cgcccgccat tcccggagcc aagccggagg tcgctgcaga ataagaggcg tcttccaaag
     6361 ttcttttgaa gaaaaactct ttgtctttga tatctataat tcgaatagtg gatcttgggt
     6421 ccagggtatt gttggcttca actgttttag gtattacgtc ctttggaacc tgaactagac
     6481 cgatattttg cgaagccaga acaggtttga aatccacttt cgaatccaaa ttgccatcat
     6541 aatccacggc accctgttct ggacgaacat aagtcacatc gacgtcgtat cgcaggatca
     6601 aaaggcactc tttttgcgca tcggcaggca aagctttaac gacgtctgcc gggcaggcgc
     6661 gcacgttgga tcccacacca ctttggagct gtcttaactg ggcatcagaa agggtgtctt
     6721 tcactttatc aagctgggtg atttcgaaaa catgaagcac ggaagtgtct aagagagtca
     6781 gcacccgcgc cttgttatca agattcaatg gaatttgata ttcattgcgc aaagtctctg
     6841 ccgtcatgat tttattgttt atacgatcca tgacaaaggt gcgatccaga tcgcctgctt
     6901 gagcgggatc gagaccgatg gctaaacggt tctcaatagt ggtgccgatt tgaatatgag
     6961 tggcttgggt ccaatccgtt tccaaaagtt caagagttga agtgtcttct ttgagcttat
     7021 ttttttcttt ttggatgacg ccaaatttga ggaccttaaa cttaaatatg ggcacaagta
     7081 gggtcgctga aggttgttgt tgactcaaaa ggtttaattt ttttgtgcgt gctttctgca
     7141 gggaagccag taactccttt gattttgcct tatctttatt tttttgcagt tgtttttgca
     7201 acagcacttc ttcttgcact tgtgccagtt gcttttcgag aattgtcagt tccatagggt
     7261 tggtcacgac tttataagcc gttacaaatt gtctatcgac cgagaaagtc accgggtaat
     7321 tatgagcagc aaggcctgtg atggtgagac cgctgaacat atacttcaaa cgaccgggca
     7381 cattgacctc agtcagagcc accactcctt tttccgccgt ggcttttgaa gagttgccta
     7441 gactcaacgt agaaagagta tcgtcggtgg ccatgctgaa ttgagagttc tcggtttgga
     7501 tatctccgaa ctccgtgatc gcgaagatgc tggcctgcat atttgcgggc aactcggcct
     7561 ctcttgattt tgtacatcct gtaccaaaca gcgctaggct tagtattacc accaaaaaag
     7621 aattagggct cataacgttc tccattcctt tagtactgca ggctcacttt ggcgtaaacc
     7681 aaagaattgt tttcgtcgat caactgataa ttagaagcgt acccattttc cttaaacaca
     7741 gagcccccgt tggtgtggcc cagagtgaaa ccaaagtgtt cccctagggc gagggaagtc
     7801 tcctcggtgt gttcaaagaa ttcttgcaag gtgtcctgat aggtccagaa gttgatatgt
     7861 gaaaaatcca cggcgaagga gaaaatgcca atagaataac ccgccgacag tccctgttta
     7921 gatgcatact tattaaggac ggttccgtcg agggccgttt catatctgtg aatattgcgc
     7981 ccaaagctga ggtctccttt aaaggaaaat cctgggatca ggagcctttc ttgaattgca
     8041 gcactgagtt ttcccgagag agcggtctct aaattattat tgatgcgaga ctctttggat
     8101 tcaggaataa cgaccgtcat cgaaggaacc agtttgaatc tggttaaatt ccagccattg
     8161 aaggccatgg cgagggcgat atcccccaca tccgagttgc cagggtttct tatatcgtca
     8221 gagaaggtga agatagctaa gcttgtgccc aagctccatt tataggaggc catgagttcg
     8281 aggtcatggg tctcctggcg ctcgccatct tgaaaattca cgaggctaat ggatttagaa
     8341 aggcccgcag aaatagcgaa aggctttact tccacaggct ttgaacctgc agttggattg
     8401 gccccagaag tgttcaaatt ttgtgcgctt tgtgcagaag attcagtgct tgaaataaga
     8461 atcccgcaaa aaagggcaaa aaatgtaaaa ctcaaggttc ttgtctgcat ataacgcttt
     8521 ccacggaatc gattttccgt ccaacattac agtgcatgcc acgggccatc tcattgaggg
     8581 acgaaatgca accaatggaa gctgatagaa tttacctggc taagctttag acatggggac
     8641 gctcctggtt gatttaaggg tttttgcatg cgattcttca gtttatcttc ctcgctataa
     8701 ggaaacctcc acgtgaaatc agaattctct gacattgttg tatttactga cggcgcttgc
     8761 tcgggtaatc ccggacctgg ggggtgggga gccgtcgtgg ttttacctga gggacaagtt
     8821 caggagttgg gcgggggaaa tgccgtcacc actaacaatc gcatggagat gctaggggtc
     8881 ataagtgctc tcaactttct ctctaagact ccgggaaata tccggattta tacggactcc
     8941 acttatgtca ttcgaggaat cactcaatgg gtgtggggtt ggcgccgcaa taactggcgc
     9001 acggctgaag gcaaggaagt ttcgaacaaa gacctctggg aagacttatt tttcttagtt
     9061 cagagacgca aagagtttaa agatctaggc gggatatctt ggaattatgt tcgggggcat
     9121 cgaggcacgc cgggaaatga acgttgcgat gagatcagcg tcgccttttc aaaaggacag
     9181 agatattctt tgtatcgcgg ttctttgtta aaatatgata ttgcaatact cgatttgccc
     9241 gaactagaag cccttcccga aatgaagcct aaaaataatg gagtcaaaga aaaagctttt
     9301 tcttatttga gcaatattgg tgggatcgtt tatcggcata aggattggtc ttcttgcgaa
     9361 cgtcgtgtga aaggcaagtc tggagccaaa tttaaaaaaa ctcaatcggc cagcgatgag
     9421 ttggaagttc tgaaatcgtg gggattgagc cctggggttg agattaagca agattaattt
     9481 tttgccattt atttttggag ccatttcggc tctgaaaata ttggtaccaa ttttgtatct
     9541 agcattcgta tgagtcgttc gtaggaacca atccatagga ggatttatga aacaaattat
     9601 gctcattgct gttctctgtc tctcaggaat tttagcccat gcccaagacg gtggacgttg
     9661 gggttgggga cgtcctggcc ctggtcgtgg cggtcctgga tatcccggac gacctggtcc
     9721 tggttatcct ggtcccggtc gtggtggcgg tggttggggc ggcaatgtcc aatgttcagc
     9781 cacggacagt ggttgggaag aacactgggg cggacacgga agttgcggtg aatgtcttca
     9841 aattcacggc aactgtacag aaacctgttc tcgtagctat aacgtttgcc aagcagaagg
     9901 catagatcaa agtggtcgtc gtttcaccac tgaaggtcgc ggtgaaattc gttatcaagc
     9961 ggaagatgat gccatgagag cttgttacta ctatcgctat cagaattgcc gaatcgtcag
    10021 ttgtaaccaa aacaatgaaa cggtttcccg cagaagttgc cgttaataaa attagcactc
    10081 tcgtgatatg gggggggcct ggccccccat tgatttttta tttcataaaa ccactaccct
    10141 actaagagct tcaaacgctt tagtatctaa agagcctaac ttaaatgttt tcatcatctg
    10201 aagggctgca actgccgatt gaggctctgg attctgcggc ccccgaatcg tgtaatcaca
    10261 aaaaacatca gctaccgata caatcaaact gaatggatga attttaggtt tgctaatccc
    10321 ctgagggaag ccttgcccca gcacgtcttc atgatgctca taggccacct gaatcacttc
    10381 cgtcggtgcg gtcttcagtg actggagaat ctcccggcct cgagtgctat gagattcaaa
    10441 taaagatcgc tctttctgtg tcaatgttga tctaggcttt tggaggagtt ccaccgggat
    10501 ctctttcttt cctatatcat ggaaaagtcc tgcgaaagaa agtttgaata gggtctgcgg
    10561 agattgccaa ccaagttgtt ttgcaatcat taccgagaac atactaaccc ccaaactgtg
    10621 actataaaga tagtcagtat ggttactcaa taagttcaga agggtaaaag tctcttcgtc
    10681 tttagttaag acttccatac ttgtgagtaa aaaatccttg gcgtttttaa acaagccttc
    10741 gtcggcgccc gcgacgtagg cctgctgcat aatcatttct ccagtgtatt tcataaaccg
    10801 catctttttt tctttatcta ctttaccact gctggaaacc gccctagata caagaaccgt
    10861 gaagccgaca agtttagaaa actcctcttg ccgaataaaa acatgagtga cttttttttc
    10921 tcgaaaggtt ttaattttgt cttctgtaag tatgccacct ttatgaacga ttttaatata
    10981 ttttgtagca gatatacgaa tatagatttc atactcagtt tccttatcgc cgatgaaatc
    11041 ctcaattggc aatttgcaaa actccttatc aaggtccagt ggtgcttgtg agatgccaat
    11101 ttgcacctct tttttttctc ccaaaattcg aaatatcttt tcaaatagtt ctaattcttt
    11161 aaaaggcttc actaagaagt ccgtagcacc taattctttt gccttttctc gatcaaggat
    11221 atgggaaaat cccgtcatta aaataaatgg agtcggtttg tatttttgag accactctaa
    11281 caaatcaaaa ccgctaaaat aaggcatttg aatatctgac agaataagat tcacgtcgct
    11341 catagacaat aggtcgcgtg ctatttttcc atcttgagcc tcaatcactt gatatttagt
    11401 ttctaatatt ttttttaaag cggacctaaa agcattatca tcttcaacaa gcaatatttt
    11461 tagcatatag gcttcttttc atgggttgat aaactggact tttcttaaaa attaattaaa
    11521 agaccattac tatgaaggct atgatttgca tctctgcaaa ttaaacacat tattttttca
    11581 catctctaac t
//
LOCUS       DATBTE010000278         1651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_720319, whole genome shotgun sequence.
ACCESSION   DATBTE010000278 DATBTE010000000
VERSION     DATBTE010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720319"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..>1651)
                     /locus_tag="VIG33_10240"
     CDS             complement(66..>1651)
                     /locus_tag="VIG33_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271255.1"
                     /translation="MPGSEASLLKRSGDSLVISLILLLPYFVQLLSSTYYIPGPDESF
                     QIQGSVQMLLHKGYASATGTIPTDLSQVQLKWITGWPIAYSFVLFLLQKLGFGALVAA
                     KFFQLGCVVLGAGGWFLVLDRLGQSKTFRCIFLFLLWAQFFSWGIYSTNAVSWALFPF
                     LIYFFLLSQADSAALWGAGAVAALMVLFRFQNVILISTGAAVLLIQAGKPFMRRIRSA
                     IIFSLIPSFCFFGIYFSNVAKSGQGTFLATIPVDITWHFRWVTDFTNAFLFNASFRFD
                     EFLRAFFEKVHFPFLFTPIKYALSFVGVTFLGIICWRNRARCDRDRRTIELIFVFQLL
                     FLVLMLFGLAVVLNVENIPNGRYYHSLFPVFVLLCLWPLRSSPYLHWLRSPRNKAALL
                     SALFLFSFGTVSYYSFYRFRLSQAFDQRAAAMEHELETAMASAPGLPTVVFADQLFPR
                     LGLRKDLTALYELGAAAKQGLRASGPVLLFLSSDERNPKSQFPVLVKEIESLSAKYGM
                     KLRKEDGHTFAWNVFPAGI"
BASE COUNT          526 a          417 c          402 g          306 t
ORIGIN      
        1 atgcctgatg cggccggtta ttggcttttt ctccagcagc tcgagcgctt tctcttccca
       61 ttgcatcata tcccggccgg aaacacattc caggcgaagg tgtgcccatc ttctttccgc
      121 aatttcatcc catactttgc cgaaagagat tcaatctctt ttacaagaac cggaaactgc
      181 gactttggat ttctttcgtc ggaggaaaga aacagtaata ccgggccgct ggcccgaagc
      241 ccctgctttg ccgcggctcc aagctcgtac aatgctgtga ggtctttccg caaacccaat
      301 cgaggaaaaa gctggtcggc aaaaaccacg gtgggcaaac cgggagcgga ggccattgcg
      361 gtttccagtt cgtgctccat ggctgcagca cgttggtcaa atgcctggga taagcgaaat
      421 cgatagaatg aataatagct caccgtgccg aagctgaaga gaaagagagc ggaaagcagg
      481 gccgccttat tgcgcgggga ccgaagccaa tgaagataag gagaagaacg caaaggccaa
      541 agacaaagca agacgaacac aggaaaaaga gagtggtagt aacggccatt gggaatattt
      601 tccacattca atactaccgc tagcccgaaa agcatcaaaa caaggaaaag cagctgaaaa
      661 acaaaaatca gctcaatggt tcgcctgtcg cgatcacatc gggcacgatt tctccagcat
      721 atgattccca gaaacgtcac accaacaaac gacaaagcat attttatcgg agtgaaaaga
      781 aagggaaagt gcaccttctc aaaaaatgcg cgcaagaatt cgtcgaagcg gaaacttgcg
      841 ttgaaaagaa acgcgttcgt gaagtcagta acccagcgaa agtgccaagt gatatccacg
      901 gggatcgtcg ctaaaaaagt tccttgaccg ctctttgcca cattcgaaaa ataaattcca
      961 aagaaacaga atgaagggat gagggaaaaa ataatggctg accggatccg ccgcataaag
     1021 ggcttgcctg cctgaatgag gagcacggca gcgccagtgg aaatcaggat tacattctga
     1081 aaacggaaaa gtaccatgag cgcagctaca gctcccgctc cccataaagc cgcggaatcc
     1141 gcctgtgaga gcagaaagaa atagatcaga aagggaaaaa gtgcccagct cacggcattg
     1201 gtcgagtaaa ttccccagga gaaaaactgc gcccataata gaaaaaggaa tatacagcga
     1261 aaagtcttcg actgacccag ccgatccagt accaaaaacc aaccacccgc tccgagaacc
     1321 acacagccta actggaaaaa tttcgctgca acgagcgccc cgaaacccag cttttgcaaa
     1381 agaaacagca cgaatgagta ggcaatcggc cacccagtaa tccatttcag ctgcacctgg
     1441 cttagatcgg ttggaatagt acccgtcgct gaagcatagc ctttatggag aagcatctgc
     1501 accgagccct gaatctgaaa ggattcgtcc ggccctggga tgtaataggt gctgctcaaa
     1561 agctgcacaa aataaggcag taaaagaatc aggctaatga cgaggctgtc accgcttcgc
     1621 ttcagcaaag acgcctccga cccaggcata a
//
LOCUS       DATBTE010000279         3565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1403572, whole genome shotgun sequence.
ACCESSION   DATBTE010000279 DATBTE010000000
VERSION     DATBTE010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403572"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..798)
                     /locus_tag="VIG33_10245"
     CDS             complement(43..798)
                     /locus_tag="VIG33_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8271256.1"
                     /translation="MEIVKPAGNQMDILFEGMPPNPKVEFMIRTKLGKVMDMSPDNSF
                     IQARVKDLGSRFGVEILVRHVTGQFLAYCEERTLGESVNVAAIQMYEQIKAWRETRYL
                     ETPQAETPFKVLIVDDDPISVKLIETCFLQRGCETHVVENGQDAIDEIFEHAYDLIVL
                     DWNMPSMNGLQTIKMLDETADLSATGIQNKKVPVLIYSASPRSQIVFPKSKTFRKIGY
                     LQKTSTYESIRKTTDAFLDSLRKIKERRLANSF"
     gene            complement(800..1324)
                     /locus_tag="VIG33_10250"
     CDS             complement(800..1324)
                     /locus_tag="VIG33_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271257.1"
                     /translation="MDFYNLQESDHRLIEKTMSLPSDSQQYKNYLFRHLFSKGFSTGF
                     SQKKFLDKKNILLMEEDPRLQCVIKNLLLEANNEAVCGIVEGIDDIICALETRPYDLV
                     IANYYLSEDEIDYEYWERIHAIYPQMEVIIFSHVNDKEYYNTLEKVANLELGPRHLPW
                     SSRLGHFFKNIFGG"
     gene            1696..>3565
                     /locus_tag="VIG33_10255"
     CDS             1696..>3565
                     /locus_tag="VIG33_10255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8271258.1"
                     /translation="MEKSIDNFTDPYHMVSENLHAFDRFLLEMQLYNHQLNGKKPLLQ
                     AFNALEESSGQYEKNKDLYQELSAIPGAIKTLQDLNTTLAELHPIIENISPIAQPRDQ
                     RRLALIYATLSPFEKHLEEINTTVYKNHVRFRSALYLKQAQSHRLLGWTMSAIAICSI
                     LLIIISHGNIKNVALLLETSKHKGTRLKEAIDQTQRANRAKSEFLATMSHEIRTPLNA
                     ILGFTSLLLDMKLSYDQKDYVQSIRASSSTLLSLINDILDVSKIEAGKLDLESIEFDL
                     RSVYVDIANIVADTVTQKKLELVSFIDSRIPRMIKGDPARLKQILLNLLNNAIKFTPS
                     GSIAVKITLLNLTEKHITLKFEVKDTGIGIEPRLLNVLFTPFTQADSSTTRRYGGSGL
                     GLSICKQLTESMGGEIGVSSIPDQGSTFWFQVKMEPVFTQPVENPLPQSFFHKDILIL
                     TNNAILKEYLELQLKEITLNPVDPLASTEGQQATAIITSTANTIAVIFDQSNSPFKQD
                     ESTKILRNISKNFEIPIIYLVEHTQNLGHLSPIQRINYLKKPVSQHNLYRCLLEVLHI
                     THIRWDDPIHNPNPQMEGTRFATLKPRVLLVEDNPINQKIALLMLEKIGCNVDVAAN"
BASE COUNT         1028 a          867 c          661 g         1009 t
ORIGIN      
        1 gttattgtta aaatccattc gtttcaaagt taagcaatcc cctcaaaatg aattcgccaa
       61 acgcctttct ttgattttcc tgagactatc cagaaaagcg tccgtcgttt tacgaataga
      121 ctcataagta cttgtttttt gaagataccc gatttttcga aaggttttac tcttcggaaa
      181 aacgatctgt gatcgagggc tggctgagta aatcaagact ggaacctttt tattctgaat
      241 ccccgtcgca ctgagatccg ctgtttcgtc caacatcttg atcgtctgca aaccgttcat
      301 agatggcata ttccagtcta gaacgataag atcgtaggca tgctcaaaaa tttcatcaat
      361 ggcatcctgg ccattttcca ccacatgggt ttcgcaccct cgctgcagaa aacaggtctc
      421 gattaatttt accgaaatag gatcatcatc aacaataagg actttaaaag gcgtttcagc
      481 ctgtggagtt tctaaatacc gggtttcccg ccaggctttt atctgctcat acatctgtat
      541 cgccgctaca ttcacacttt ctcctaaagt tctttcttcg caataagcta aaaactgccc
      601 cgtcacatga cgaaccagaa tttccactcc gaaacgggaa cccaagtcct tcactcgtgc
      661 ttggataaag gaattgtccg gagacatatc catgactttc cccaatttag tcctgatcat
      721 aaactctact tttggattcg gaggcatccc ctcaaagaga atatccattt gatttccggc
      781 gggctttaca atttccatat taacctccga atatattttt aaagaaatgt cctaatcgag
      841 aagaccaggg gagatgcctg ggacccaact caagattcgc caccttttcc agggtgttat
      901 aatattcttt atcattcaca tgtgaaaaga tgatcacttc catttgtggg tagatggcat
      961 gaatacgttc ccaatactca taatcaattt catcttctga gagataataa ttggcaatca
     1021 ctaaatcata aggtcgcgtc tccagagcgc agatgatatc gtcgattcct tccacaatcc
     1081 cacaaacagc ttcgttatta gcttcaagaa gtaaattctt tatcacgcac tgaagtcgag
     1141 gatcttcttc cattagcaag atattttttt tatccagaaa cttcttctga gagaaccccg
     1201 tagagaaccc cttggagaag aggtgacgaa atagataatt tttatactgt tgtgagtccg
     1261 aaggaaggct catggtcttc tcaataagac gatgatcgct ctcctgtaaa ttataaaagt
     1321 ccatgatccc cctcatgaat aacgtgataa aacctttgtt ataggtcaga ctattattct
     1381 ttaataccat cgaaacggtt tgaatttcac gtgaagaaat gccgacaact taccagtttt
     1441 ctgtgagcga attatcactc attttctaac aaacactcat aagtgtgagt ttttcttcac
     1501 aggatggact cgaaatttgt tttctttctt ggagcggcta ttgtaattat ccttatgctt
     1561 caattattta aaaaggaacc cagactgagt gccgcctttt tccccaagct tccctacatg
     1621 tttttgattg tttttttaat cattttctca gggggtattt ctgttctgta ttatcgaact
     1681 ttcccagaca gccatttgga aaaaagcatc gataatttta ccgatcctta ccatatggtc
     1741 tcagagaacc tccacgcttt cgatcgattc ttgctcgaga tgcaacttta taaccatcaa
     1801 ttgaatggta aaaagccgct tttgcaggcc ttcaacgctc tcgaagaaag ctccggtcaa
     1861 tatgaaaaaa ataaagatct ctatcaagaa ctcagcgcca tacccggagc cattaaaact
     1921 ctccaggatc tcaacacaac cttggcggag cttcatccta tcatagagaa catttcgccg
     1981 atcgctcaac caagggatca gcgcaggctg gctctgatct acgcgacttt atctcctttc
     2041 gaaaagcacc tggaagaaat aaatacaacg gtttacaaaa atcatgtccg tttccgttcc
     2101 gccctttacc taaaacaagc ccaatcccac cgcctgctag ggtggactat gtcggctatt
     2161 gccatctgca gtattctgct cattatcatc tcccacggaa atattaaaaa cgtggctttg
     2221 ctcttggaaa cttcgaaaca caaaggcacc cgcctgaaag aggccattga ccaaactcag
     2281 cgggccaacc gtgcaaagtc cgaattcctg gcgacgatga gccatgagat tcgcactccg
     2341 ttaaatgcca ttctcggctt tacctctctg ctattagaca tgaaattatc ctacgatcaa
     2401 aaagactatg ttcaatccat aagagcttcc agcagcactc tgctctccct gatcaatgat
     2461 attttggatg tttcaaaaat tgaggccggc aagcttgatt tggagtccat tgagtttgat
     2521 ctgcgttccg tctatgtgga tatcgccaat atcgtggcgg acaccgtgac tcaaaaaaaa
     2581 ctggagcttg tgagctttat cgattccaga attccgcgaa tgataaaagg cgatcccgct
     2641 cgccttaagc agattcttct gaatctttta aacaacgcga ttaaattcac gccgagcgga
     2701 agtatcgccg tgaaaatcac gcttttaaat ctcacggaaa aacacatcac cttgaaattc
     2761 gaagtgaaag acaccggaat cgggattgaa ccgcggctcc tgaatgtcct tttcacccct
     2821 ttcacgcaag cggactcatc aaccaccagg cgttatggag gatcgggctt agggctttcc
     2881 atttgcaagc aactgacgga atccatggga ggggaaatcg gcgtcagcag cattccggat
     2941 caaggctcca cgttttggtt ccaggttaag atggagcccg ttttcactca acctgtggag
     3001 aatcctcttc ctcaatcttt ctttcataaa gacattctca ttctaaccaa taatgcgata
     3061 ttaaaagaat atcttgaatt gcaactcaag gagatcaccc tcaaccctgt cgatcccctc
     3121 gcgagcacag aaggacagca agccactgcc attatcactt ccactgcgaa tactatcgcc
     3181 gtcatcttcg atcaaagcaa ttcacccttt aagcaagacg aatcgacaaa gattcttaga
     3241 aacatttcaa aaaattttga aatacccatt atttatttgg tggagcacac gcaaaatttg
     3301 ggtcaccttt ctccgattca acgaatcaac tacttgaaaa aaccggtgtc tcaacacaac
     3361 ctttataggt gtctcttaga ggtcctgcat atcactcaca ttcgttggga tgaccccatc
     3421 cacaacccca atccacagat ggaagggacc agatttgcca ccctaaagcc cagggtgctg
     3481 ctggtggaag acaatcctat taaccaaaaa atcgctcttt taatgctaga aaaaattggt
     3541 tgcaatgtag atgtggcggc taatg
//
LOCUS       DATBTE010000280         6025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1403607, whole genome shotgun sequence.
ACCESSION   DATBTE010000280 DATBTE010000000
VERSION     DATBTE010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403607"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VIG33_10260"
     CDS             <1..577
                     /locus_tag="VIG33_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY8271259.1"
                     /translation="LPELNDEFLATLGGPTNLEELKKTIREDLENTEKKRIEDAFKNR
                     LLKKLVKENPVEVPASLLKNQKNSLIDDFKKRMDEQGMSPADFKDYVAKWDSDFEKTA
                     SEMIQSSFLVDAIAKKHDLYCKDADIDKKYQEYSAQTGIDEARIREFYARPEQTSRLS
                     YMITEEKVIDFLNQKIKVKEVSADQLKDEQI"
     gene            564..1172
                     /locus_tag="VIG33_10265"
     CDS             564..1172
                     /locus_tag="VIG33_10265"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEY8271260.1"
                     /translation="MSKSKSELEEILSQGPHTDLSKTFSKASGVALLLCNTGANTEID
                     PLSRLELLFIKRATNPKDHWSGHIAFPGGKQDEGDPSLLAACLREVQEEVGLHLSEDS
                     LIGFLDDLQARKHGNLLEFYIRPFVFSLKEKPFVKAVADEVEKTLWVPLNYLTDTKNH
                     ITYTLTRDQIKLELPGIQLPDGEVLWGLTYTMVQNLLRNLQA"
     gene            complement(1169..1738)
                     /locus_tag="VIG33_10270"
     CDS             complement(1169..1738)
                     /locus_tag="VIG33_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271261.1"
                     /translation="MKQAFSIFFLLGLILSTGFAADETSVGFSQGNRFYSSHLQGTVT
                     VYCESGETVTYTCNDTVLDPSSYDYFVGPQGVKAEEVSLTSTRADGSRRERTESYNSK
                     TPRSMSAFNLWISTPFQRPLLGLGSNKIDYRLKSAGKTVDQGAFSVSVTYGGPRTCPA
                     THYNSNDASDCQSPYTICQKYFEQYDHCR"
     gene            complement(1799..3187)
                     /locus_tag="VIG33_10275"
     CDS             complement(1799..3187)
                     /locus_tag="VIG33_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY8271262.1"
                     /translation="MKAFILKWKTFILALILLLSLFMLAEAGFQVRAFAQDRYADLQN
                     FTKVLNLIEQYYVEEVDTKKLLYGAIKGMLRELDPHTNFMPPDIFKDFETETTGEFGG
                     LGIEISVTNGILTIISPIEDTPAWEAGIKPGDKVIAVDGHSTKGLSLVEASQLMKGKK
                     GTKIVLRIVRESEEEPKDITITRGSVKIKSVKYTNLEDGYAYIKITSFIENTAKDLEK
                     VIDAHIKNNQKVDGLIIDMRRNPGGLLDQAVKVSDMFLKEGTIVSTIGRNKKDKEVSV
                     ASKKGRFTDFPIIILVNEYTASASEIVSGALQDNKRALIAGQRTFGKGSVQSVVKLGD
                     GSGLKLTVARYYTPSGISIQAEGIKPDIELEDVDPEAFAKSVLKSKPTREKDIAGHLK
                     GDREKDTEKIEKKLDLKPKEPSNAFAWWKEVGNKKDENLTPREKLFKNDYQVYQALSY
                     LKAWKTLKTLAR"
     gene            complement(3184..4332)
                     /locus_tag="VIG33_10280"
     CDS             complement(3184..4332)
                     /locus_tag="VIG33_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY8271263.1"
                     /translation="MIRICSGILCWFCSFNLAFASAEIPAREPTPDQNLEKIAKDLQI
                     AKMNLEIQELKQKKVLGALYGINKNLKKLVSETGSLEQQRNVIGLQIQQLNKKIGDND
                     QLITAQKLLLTTRLHAIYKLKGPTLARFLFAAKSSADLERNLKILGVVARRDLNLIKT
                     YRESVKDLHYRRDRLAQKMDSLKEVELNLQTYAKKLQKEISQKNSILAGIRSTKMFTQ
                     DQIKSLKNKSLSYDIADSGLMDLLLRPSFEAQKGILPKPTQGPVMKTFGLAKSPDQSY
                     VLSNKGLFIAATPGSPVRAVFSGVVSFAGSLPGLGQVLILDHGDHYYTIYGNNASLRV
                     QLGQEVTQTQIIASSGRSIFDQQNGLYFEVRHFSEPYNPMEWVKGFSQ"
     gene            complement(4329..5249)
                     /locus_tag="VIG33_10285"
     CDS             complement(4329..5249)
                     /locus_tag="VIG33_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEY8271264.1"
                     /translation="MVRSLFQQRIGSLKRNWTLQFSTLIVVTACYLIVCSSMLLSQNL
                     RKILTVWGEELQMTVYLAENAEKTETESLLKTIQADLNVGRIKYISKEKALEDFRGQM
                     ASYAPDILNEKDLLSLIPASFQVSLRESLKATDHLKVLDSLAENLKIQKSVAEVSYGQ
                     EWVQKYSQFLYYFEKACQALGVIIFCAALFVLSNVIRASVQSRRQEIEVLELIGATPG
                     MIRRPFMVEGALIGFASSLMAIGVSYFAYTNVVRLFENELQFLQLGQHLHFLSFSLIA
                     LFCSAGTLLGALGSYLCVRQINDGWAARSL"
     gene            complement(5249..5908)
                     /gene="ftsE"
                     /locus_tag="VIG33_10290"
     CDS             complement(5249..5908)
                     /gene="ftsE"
                     /locus_tag="VIG33_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEY8271265.1"
                     /translation="MIEFSHVYKTYPGPIHALKNIDLQIGKGGFVFLTGPSGAGKTSL
                     FKMISAFDTSTSGVVKVAGYDINNLSENKIAEYRRKIGVVFQDFRLLKDRTVFENVAL
                     PLQIRGDRPMSIQRRVQEVLEQVGLTHKYDQFPDFISGGEQQRTAIARAIVHQPGVLI
                     ADEPTGNLDPQLSEEIMDLLERVCSQGTTVFIATHDHDMVKRRNKRVIQLKDGLIVGD
                     Q"
BASE COUNT         1755 a         1336 c         1175 g         1759 t
ORIGIN      
        1 attgcctgag ctgaacgatg aattcctagc tacgttgggc ggtccaacaa atcttgaaga
       61 gttgaaaaaa acaattcgtg aagacctcga aaacacagag aaaaaacgta ttgaagacgc
      121 cttcaaaaat cgtctcctta aaaaattagt taaagaaaat cccgtggaag ttccagcttc
      181 tcttttgaaa aatcaaaaaa attctttgat tgatgatttc aagaaaagaa tggatgagca
      241 aggaatgtct ccagctgatt tcaaagatta tgtagccaag tgggacagcg attttgaaaa
      301 aacagcctcg gaaatgattc aatctagttt cttggttgat gccatcgcca aaaaacatga
      361 cctttactgc aaagatgccg atatcgataa aaagtatcag gagtattcgg ctcagacagg
      421 gattgatgaa gctcgcatcc gcgaattcta tgcccgtcct gaacaaacaa gccgtttgag
      481 ctacatgatt actgaagaga aagtgatcga cttcttgaac caaaaaataa aagttaaaga
      541 agtgagcgcc gatcaactca aagatgagca aatctaaaag cgagttagag gaaattctct
      601 ctcaagggcc gcacactgat ctttcgaaaa ctttttcgaa agcatcagga gtggctcttt
      661 tgctatgtaa cactggagcg aatactgaaa ttgacccgct ctctcggtta gaactgctct
      721 ttatcaaacg ggctacaaat cccaaagatc attggtccgg tcatattgcg tttccgggtg
      781 gaaaacaaga cgaaggagat ccttctttat tagcagcctg ccttcgggag gttcaggaag
      841 aagtgggtct gcacctgagt gaagactcgc tcattggatt tttagacgat ctacaggctc
      901 ggaaacatgg aaatttgctg gagttctaca ttcgcccttt tgtcttctct ttaaaagaaa
      961 agccttttgt aaaagccgtc gctgatgaag ttgaaaagac gctgtgggta cctttaaatt
     1021 atttaacaga taccaagaac catataactt acacactcac gcgagatcaa ataaagctcg
     1081 aacttcccgg aattcaactt cctgacggag aagttctctg gggcctcact tatacaatgg
     1141 tccagaatct attaagaaat ctgcaagctt aacgacagtg atcgtattgc tcgaaatact
     1201 tttggcaaat ggtgtacggt gattggcaat cgctcgcatc gttagagtta taatgagtcg
     1261 cgggacaagt tcgaggccca ccataagtca cacttactga gaaggctcct tgatcaacgg
     1321 tttttcccgc gctctttagt cgatagtcga ttttgttaga tccaagacct aacaaaggtc
     1381 tttggaaagg ggtggatatc cacaaattaa aagcgctcat actccgcggg gtctttgaat
     1441 tatagctttc cgtccgttca cggcgacttc catctgctct tgtgctagtt aaagaaactt
     1501 cctccgcttt gaccccttgg ggtcccacaa aataatcata actggaagga tccaaaactg
     1561 tatcattgca tgtataagtg acggtttctc cagactcaca gtaaacagtc actgtccctt
     1621 gcaaatgaga ggaataaaag cgattccctt gagaaaagcc aacactggtt tcgtcagcgg
     1681 caaagcccgt acttaaaatt aaacctaaca aaaaaaatat agaaaatgct tgcttcatag
     1741 ggacctcatt ttttgactag actaaaaggt ctaatctgaa atagcaatcc ctagtagctc
     1801 agcgcgccag agtcttcaat gtcttccagg cctttaaata ggataaagct tgataaacct
     1861 gatagtcatt cttgaagagt ttttcacgag gcgtgaggtt ttcgtctttt ttattaccaa
     1921 cttctttcca ccatgcaaat gcattgcttg gctctttagg ttttaaatcc agtttctttt
     1981 caatcttttc ggtatctttc tcgcgatccc cttttaagtg accagcaata tctttttctc
     2041 gagtgggttt agattttaat accgactttg cgaaggcttc aggatccaca tcttcgagct
     2101 caatatccgg tttaattccc tccgcctgaa tactaatccc acttggagtg taataacgag
     2161 cgacggttaa ctttaatccg cttccatctc ccaattttac taccgactgc acggaacctt
     2221 ttccaaaggt acgctgaccc gcgatcagtg cacgcttgtt atcttgaaga gctccggaaa
     2281 caatctcact tgcggaggcc gtgtattcat ttacaagaat gattattgga aaatcggtaa
     2341 aacgaccttt tttagaagcc actgaaactt ctttatcttt tttattgcgt cctattgtac
     2401 tgacgatagt tccttctttt aagaacatat cgctgacctt caccgcttga tccagcagcc
     2461 cccccggatt acggcgcata tcaataatta aaccatctac tttttgatta ttcttaatat
     2521 gtgcatcaat cactttctca agatccttgg ccgtgttctc gataaagctg gtgattttta
     2581 tataagcata accatcttca agattggtgt actttactga tttgatttta acactgccac
     2641 gagtgatcgt aatatcctta ggctcttctt cgctctcgcg aacgatgcgc aaaacaattt
     2701 tggtgccttt tttacccttc attaattgtg aggcttccac cagacttaaa ccttttgtac
     2761 tatgaccgtc cactgctatg actttatcgc cgggtttaat ccccgcctcc caggctggcg
     2821 tgtcttcgat cggagaaatg atcgtaagaa ttccattggt cactgaaatc tcgattccca
     2881 atccaccgaa ctcaccggtg gtttccgttt cgaaatcttt gaaaatatcg ggaggcataa
     2941 agttcgtgtg gggatccaac tcacgcaaca ttcctttaat ggcaccataa agaagttttt
     3001 ttgtgtccac ttcttccaca taatattgct cgatcaagtt caaaacttta gtgaaatttt
     3061 gtaagtcggc ataacgatct tgagcgaaag ctctcacttg aaatcccgcc tctgccagca
     3121 tgaacagcga taaaagcaaa atcagtgcga gtataaatgt tttccatttg agtataaatg
     3181 ccttcattgg ctaaatcctt tcacccattc catcggatta tagggctctg aaaaatgtcg
     3241 aacttcaaaa tataatccat tttgctggtc aaaaatgctt cgtccgctgc ttgctatgat
     3301 ctgcgtttgc gtcacttctt gtcctagctg aacacgtaag ctcgcattat taccatagat
     3361 agtataataa tgatctccgt gatcgagtat taaaacttgg ccaaggccag gcaaacttcc
     3421 tgcaaaagat acgactccgc taaaaaccgc acgaactgga ctgccaggag tcgctgcgat
     3481 aaaaagtcct ttattactta aaacgtaaga ttgatcagga gatttcgcca aaccaaaagt
     3541 tttcatgact ggaccttggg ttggtttagg caaaattcct ttttgggctt caaaggaagg
     3601 tcttaataaa agatccatga gacctgagtc ggcaatatca taactaagtg atttattttt
     3661 taaggacttg atttgatcct gggtaaacat tttcgtgcta cgaattccgg caagaatgga
     3721 gtttttctga gaaatttctt tttgcaattt ctttgcataa gtctgaaggt tcaattccac
     3781 ttcttttaaa gagtccattt tttgagccag acggtccctt cgataatgaa gatctttgac
     3841 ggattcccgg taagtcttaa tcagatttag atctcttcgc gcaacgacgc ctagaatttt
     3901 gagattcctc tctaaatctg cagaactttt cgctgcaaat aagaaacgag ctaaggtggg
     3961 gcctttgagc ttataaatag cgtgtaaccg tgtggtcagc aataattttt gagctgtgat
     4021 caattgatca ttgtcgccga tcttcttatt gagttgctga atctgaaggc caatgacatt
     4081 tctttgttgt tctagacttc cagtttcact aacgagtttt ttcaagtttt tattaatacc
     4141 gtaaagagcg ccgagaactt tcttttgttt aagttcttgg atttcgagat tcatcttggc
     4201 aatttgtaag tctttggcaa ttttctccag attctgatca ggtgttggct cccgcgcagg
     4261 tatttcagct gacgcaaaag ccagattaaa agagcaaaac cagcataata tccctgaaca
     4321 aatcctaatc ataaacttct tgccgcccac ccatcattga tctggcgaac acaaagataa
     4381 gatcctagag cccctaacaa agttcccgct gagcagaaaa gcgcaattaa actaaaactt
     4441 aagaaatgta agtgctgacc caattgcaga aactgcaatt cgttttcaaa aagccttaca
     4501 acgttggtgt aggcaaaata agacactcct atcgccatga gcgacgaagc aaagccaatg
     4561 agagctcctt caaccataaa aggacgccga atcatcccgg gggtggcgcc aataagctct
     4621 aatacttcaa tttcttgacg acgggattga accgacgcac gaattacatt tgaaagtaca
     4681 aatagggccg cacagaagat aataactccc aaggcctggc aggctttttc aaaatagtat
     4741 aaaaactggc tgtacttttg cacccactct tgaccgtaac ttacttccgc caccgacttt
     4801 tggattttta aattttccgc taaagagtcg agaaccttta aatgatccgt ggctttaaga
     4861 gattcccgca aagacacctg aaaacttgct ggaatcaaac tcaaaagatc tttctcgtta
     4921 agaatatccg gggcataact ggccatttga ccacgaaagt cttcgagagc tttttcttta
     4981 ctgatatact taatcctgcc tacattcaga tcagcttgaa ttgtttttaa taaagattct
     5041 gtctctgttt tttctgcatt ctctgcaagg taaactgtca tttgcagttc ttctccccat
     5101 accgtcagaa tcttgcgaag attttgcgac aataacatcg aactacaaac aatcagataa
     5161 catgccgtca ctacgatcaa agtagaaaac tgcaaggtcc aatttctttt aagacttccg
     5221 attctttgct gaaagagaga ccgaaccatt attgatctcc cactatcaag ccatctttca
     5281 attgaatcac acgcttattt cttcttttta ccatatcgtg atcatgagtg gcgataaaca
     5341 ccgtggtgcc ttgtgaacaa accctctcca agagatccat gatttcctca ctgagttgcg
     5401 gatcgagatt tcccgtgggc tcatcggcga tcaaaactcc tggttgatga acaatcgcac
     5461 gagcaatcgc ggtccgctgt tgttctcctc cggagataaa gtcaggaaac tgatcgtatt
     5521 tatgagtgag acccacctgc tccaagactt cttggactct tctttgtatg gacatgggac
     5581 ggtcaccgcg aatttgtaaa ggcaaggcta cgttctcaaa aacagtgcga tctttcagaa
     5641 gacggaaatc ttgaaaaacc acaccgattt tgcgtcggta ttcggcgatt ttgttctcgc
     5701 tcaagttgtt aatatcgtag cctgcaactt tcaccactcc cgaagtcgac gtatcaaagg
     5761 ccgaaatcat tttaaatagg gaagtttttc cggcaccgct cggcccagtg agaaagacaa
     5821 atcccccttt gccaatttgc aaatctatat ttttaagcgc gtgtatcgga cctggatagg
     5881 ttttataaac atgtgaaaat tcaatcatca ttttattcta gggaaggaat cacttgtttc
     5941 ctagaaacct ctggtctagt accgacaaga gaccagagag gaaagacaaa agtctaagaa
     6001 aatgaggaag cccatgaaga tcttt
//
LOCUS       DATBTE010000281         6673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2360422, whole genome shotgun sequence.
ACCESSION   DATBTE010000281 DATBTE010000000
VERSION     DATBTE010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2360422"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VIG33_10295"
     CDS             complement(<1..707)
                     /locus_tag="VIG33_10295"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8271266.1"
                     /translation="MKRCLACNAQFKSFLTGCPSCGREPSFVEGFCSYAPQFAQGGGG
                     FKAKYFSDLAQLEAANFWFQARNQFILWAIEKYSPNFQSLLEIGCGTGYVLSGIAKKF
                     PHATLQGSEIFTTGLGFAASRLPATSFIQMDARNIPFKEEFDILGAFDVLEHIQEDEE
                     VLAQMYAALKPQGILLITVPQHAWLWSVMDEYACHVRRYAASELHRKIEVTGFQIIRS
                     TSFVSTLLPAMVVSRFF"
     gene            complement(785..1708)
                     /locus_tag="VIG33_10300"
     CDS             complement(785..1708)
                     /locus_tag="VIG33_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271267.1"
                     /translation="MKKKWQKLGVIYCPSGAHRHPKLLTHAANPLPVLIEGDIYRLFF
                     SGRDAANRSSVGAVDFDIVKRKVVCEHFDPFFEHGPAGSFYADGVSIGNCYDVDGVKY
                     MLFMGWQNSPGSHWRGDVGRLVVNPDLTLKLAQEEPFLGSNSMDPLSLSYPWVLKNEE
                     DGYRMWYGSTVNWDAGNGEMLHVIKSAFSVDGHHWHREGLALPYELGRAQAFSRPAVL
                     GNSKRGYEMWFSYRSGQGEQYRIGYALSKEGKTWDLKLDEVGMDPSLEGWDSEMMEYP
                     FVFDHKGQRYMLYNGNGYGKSGFGLALLTYD"
     gene            complement(1705..2370)
                     /locus_tag="VIG33_10305"
     CDS             complement(1705..2370)
                     /locus_tag="VIG33_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEY8271268.1"
                     /translation="MKKPLVIFGYGDIAQLAYFYFSSDSEFEVVAFTVDAAYRKESSF
                     CGLPVVAFEEVAEQYPPSEHDLFVALSYAKLNAVRKEKYLAAKLKGYRLASFVSSHAT
                     VLNEGRIGENCFILEDNTIQPFVVIGNNVTLWSGNHIGHHSLIKDHCFIASHVVVSGG
                     VEIGEQCFIGVNATLRDHIKIGEKCVLGAGTLLLADAEAEGVYIGTATPRSEMLSAKL
                     KKI"
     gene            complement(2367..3005)
                     /locus_tag="VIG33_10310"
     CDS             complement(2367..3005)
                     /locus_tag="VIG33_10310"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8271269.1"
                     /translation="MQNKKNNLLAEIAGYYTSKLSQHGETPQGVDWNGEESQTQRFRQ
                     LCKIIDPSKKDFSINDLGCGYGALYDFLALHYEAFSYSGVDVSASMVRAAEQRHQNKT
                     NARFLLTSEPDQIADYGVASGIFNVRLSHSETEWVKYIETILDALDRTSRMGFSFNCL
                     TLYSDVEKRRDHLYYADPGILFDFCKRRYSRHVALLHDYGLFEFTILVRKQL"
     gene            complement(2986..3915)
                     /locus_tag="VIG33_10315"
     CDS             complement(2986..3915)
                     /locus_tag="VIG33_10315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8271270.1"
                     /translation="MKLSIVATLYRSEAYIPEFHQRASSAAKQLVGENYEIILVNDGS
                     PDGSLDIAVKIAEKDEHSVVVDLSRNFGHHKAMMTGLAHSKGQVVFLIDSDLEEEPEW
                     LLRFSDQLAKDGCDVVYGQQEQRKGNLFERWSGQWFYRVFNKLTGIALPENIVTARLM
                     TRRYVDALLRHEEREVFMAGLWYITGFTQHSQIIKKHSTSETTYTFRRKMSLLVNSVT
                     SFSNAPLVSIFYIGVSISLVAMFYIVYLILYWMFFAKPLGGWTSVMASIWLLGGMIIS
                     FIGVVGIYLSKIFAETKRRPYTIVREVYAKQKK"
     gene            complement(3912..4607)
                     /locus_tag="VIG33_10320"
     CDS             complement(3912..4607)
                     /locus_tag="VIG33_10320"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HEY8271271.1"
                     /translation="MKKVAILQSNYIPWKGYFDLIAAVDELIIYDDMQYTRRDWRNRN
                     QIKTPQGLQWLTVPVQVKGKYHQKIKDTEIDGSDWALTHWKALVQNYRRAAYFNEIAV
                     WLESLYFEGPFTNLSLLNRRFIEAICNYLNIETVISNSWDYTLLDGKMERLVDLCVQA
                     GATEYISGPAAKDYIVESLFSERAIELTWFDYSGYPQYPQLWGEFSHQVTILDLLFNC
                     GKDSHRSMRYVRE"
     gene            complement(4604..6064)
                     /locus_tag="VIG33_10325"
     CDS             complement(4604..6064)
                     /locus_tag="VIG33_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271272.1"
                     /translation="MGFNLWDEGFLWYGAQRTMLGEVPIRDFMAYDPGRYYWSAGFMS
                     LGGDNGIMSLRASVAIFQTIGLFVGLFLIAHTVKKQNIFFMILSALTLVVWMYPRHKL
                     FDISLSIMFIGVLAFLIQRPILRRYFFAGLCLGLVAIFGRNHGVYGFVGSLGSLAWLS
                     LKTDKGSEVIKGSALWLAGVVVGFSPILFMALLMPGFAAAFWESIRFLFEVRATNLPL
                     PVPWPWTIHFDLLPLDQALRGVFVGLFFIGVVVFGVLSILWVVRKKIQHKQVSPVLVA
                     TAFLALPYAHYAYSRADVGHLAQGIFPLLVGCLVLLVDQPGKIKWPLGLALCGASLWV
                     MHVFHPGWQCQIDKHCVDVEISGSHLQIDPESAEDVGLLRKLVHQYAPNGQSFIATPF
                     WPGAYALFERKSPLWEIYAFFLRSPDFEQAEIERIKAAKPGFALVFDFPLDGHDELRF
                     QRTHPRIYRYILDNFDRVPDSPNRAYEIYKARSDVQ"
     gene            complement(6147..>6673)
                     /locus_tag="VIG33_10330"
     CDS             complement(6147..>6673)
                     /locus_tag="VIG33_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271273.1"
                     /translation="GEAWDLVPQNIIVGQDGKYAIIKPEWFYKESFRKSWFILRCILQ
                     FDQNIHTILCEKFGSLKEFYFFSCGLLEVSGDLALDVSIELKTQDEKFGMGRGPMKTP
                     LEEYLAWNLASGGSAFTRDSRFILAAISTERESSLQLRKSLAELSEKYSQLVNLKLVR
                     LGLCLLRITKKVFS"
BASE COUNT         2032 a         1727 c         1311 g         1603 t
ORIGIN      
        1 tgaaaaaatc tagaaaccac cattgccggc aacagagtgg agacaaaaga tgtactcctt
       61 attatctgaa acccagtcac ttctattttg cggtgaagct ccgaagctgc atagcgccgg
      121 acatggcaag catattcgtc catcacactc cacagccaag cgtgttgggg cacggtgatc
      181 agcaggattc cctggggttt taaggccgca tacatctgcg ccagaacttc ctcatcctct
      241 tggatatgtt ctaggacatc gaaggcccca agaatatcga attcctcttt aaaaggaata
      301 ttcctggcat ccatttgaat aaaactcgtg gctggcagcc ttgaagccgc aaacccaaga
      361 cccgtggtaa aaatctcact tccctgaaga gtggcgtgcg gaaatttttt tgctattcct
      421 gacaagacgt agccagtgcc gcaaccgatt tctaagagag attggaaatt tggagaatac
      481 ttctcaatgg cccaaagaat gaattgattt ctagcctgaa accagaaatt agccgcttcc
      541 aattgcgcca aatctgaaaa atactttgcc ttaaagccac ctccgccctg ggcaaattgg
      601 ggcgcataag aacaaaagcc ctccacaaag gaaggctccc ttccacagct tggacagcct
      661 gtgaggaatg atttaaactg tgcattgcag gcgagacagc gtttcatagg cacccttcat
      721 cattgttctc gtctttattc tcgtcattgt tctcgtcttt agaggacgaa tggcaatcca
      781 tccattaatc gtaagttaaa agagctaaac caaagccact tttgccgtag ccatttccat
      841 tgtaaagcat ataacgctgt cccttatgat caaaaacgaa gggatactcc atcatctctg
      901 agtcccagcc ttccaaagaa ggatccatcc caacttcgtc taacttcaag tcccacgtct
      961 tcccttcctt actcaaggcg tatccgatac ggtactgttc tccttgacca ctgcgatagg
     1021 aaaaccacat ttcataacct ctttttgaat ttcctagaac agccggtcgg gagaatgcct
     1081 gagcccttcc aagctcataa gggagcgcca aaccttcgcg atgccaatga tgtccatcaa
     1141 cggagaatgc ggacttaatg acatgtaaca tttctccgtt gccggcgtcc caatttaccg
     1201 ttgagccata ccacatacgg taaccatcct cttcgttttt cagtacccag ggataagaaa
     1261 gacttaaagg gtccatcgaa ttagaaccga gaaagggttc ttcttgagct aattttaaag
     1321 tcaggtccgg attcacaacc agccgcccca catcaccccg ccaatggctc ccgggggaat
     1381 tctgccatcc cataaaaagc atatacttca caccatctac gtcgtaacaa ttaccaatac
     1441 tcaccccatc agcataaaaa ctgcctgccg gcccatgctc gaaaaacgga tcaaagtgtt
     1501 cacaaacaac cttgcgcttt acgatatcaa aatcaaccgc ccccaccgaa gaccgattag
     1561 cagcatccct gccactaaaa aaaagacgat aaatatctcc ctcaatcaga acaggaaggg
     1621 gatttgctgc atgagttaaa agtttcggat gtcggtgagc accggaagga cagtagataa
     1681 cccccagttt ttgccatttc tttttcatat cttttttaat tttgcactga gcatctcaga
     1741 gcgcggagtg gctgttccaa tatacacccc ctcagcttcc gcatccgcca acaatagggt
     1801 cccggcccca aggacacact tctcaccaat cttaatatgg tcccgcaagg ttgcgttgac
     1861 accgataaaa cactgttcac caatctcaac cccccccgag accacaacat gcgacgcgat
     1921 aaaacagtga tccttaatga gagagtgatg accgatatga ttcccactcc acagtgtcac
     1981 attattgcca atgacaacaa acggttgaat tgtgttgtct tctaaaatga aacagttctc
     2041 gccaatacga ccttcattta atacggtggc gtgcgaactc acaaagctcg ccaatctata
     2101 accctttaat tttgccgcca gatacttttc cttccttacg gcatttaatt tggcgtaact
     2161 gagagccaca aataaatcat gctcactggg ggggtattgc tcggcaactt cttcaaaggc
     2221 gaccactgga agtccgcaaa aagaggattc cttgcgatag gcggcatcga ccgtaaaggc
     2281 aaccacttcg aactccgagt cagaactaaa ataaaaatag gcaagttgag caatgtcccc
     2341 ataaccaaaa atcactaaag gttttttcat agctgtttcc tcaccaagat cgtaaattca
     2401 aataatccat aatcatggag taaagccacg tggcgtgaat agcgacgctt acagaaatca
     2461 aataaaatac ctgggtctgc gtaatagagg tggtcgcgcc tcttttccac atcagaatag
     2521 agggtcaggc aattaaaaga aaaacccatc ctactcgttc gatccagagc atccaatatg
     2581 gtttcaatat atttcaccca ctccgtctct gagtgactca agcgaacatt gaaaatacca
     2641 ctggccacac cgtagtccgc aatttgatcc ggttcgctgg taagaaggaa tcgcgcgttg
     2701 gttttatttt gatggcgctg ctcggccgct cggaccatgc tcgcggacac atcaaccccc
     2761 gaataagaaa aggcttcgta atgaagcgct agaaaatcat agagtgcgcc ataaccgcaa
     2821 cccagatcat taatggaaaa atcctttttt gaagggtcga taattttaca gagttgtcgg
     2881 aagcgctgag tttgactttc ttctccgttc caatccactc cttgcggagt ttcaccatgc
     2941 tgtgacagct tggacgtgta atatccggca atctcggcca ataaattatt ttttttgttt
     3001 tgcatacacc tcccttacga tggtgtaggg tcgccgcttt gtttctgcga aaattttcga
     3061 gagataaata cccaccacgc cgataaaaga aataatcata ccgcccagaa gccagatcga
     3121 cgccatcacc gaagtccagc cacctaacgg cttggcgaag aacatccagt acagaatcag
     3181 ataaacaata taaaacatcg ccaccagaga aatggaaact ccgatataaa agatactgac
     3241 cagcggagca ttactgaaag aagtcactga attgaccaac aaagacattt ttctgcggaa
     3301 agtgtaggtg gtttcactgg tgctgtgctt ttttattatt tgggagtgtt gagtaaagcc
     3361 tgtaatatac caaagacccg ccatgaagac ttcccgctct tcatggcgca ataaagcatc
     3421 cacataccga cgtgtcatca agcgtgcggt aacaatgttt tccggaagag caatgcccgt
     3481 gagcttgttg aaaacccggt aaaaccactg cccactccaa cgctcaaaga gatttccttt
     3541 gcgctgctct tgttgtccat acacgacatc gcacccgtcc tttgccagct gatcagaaaa
     3601 gcggagcaac cattccggtt cctcctccag atcgctgtca attagaaaaa caacctgtcc
     3661 tttggaatga gcaaggcctg tcatcatcgc tttgtgatga ccgaaattgc gggacaaatc
     3721 aaccacgaca ctatgctcat ccttctcagc gattttcaca gcgatgtcga ggctaccgtc
     3781 gggcgatcca tcattgacca gaattatttc ataattttca cccaccagtt gcttcgcagc
     3841 agaactggcc cgttgatgaa actcagggat ataggcctca gaacggtaaa gagttgcaac
     3901 aatggaaagc ttcattcgcg tacatacctc atggagcggt gcgagtcttt accgcagtta
     3961 aacagtaaat caagaatagt cacttgatgg gagaactcac cccaaagctg aggatactga
     4021 gggtatcccg aataatcaaa ccaagtcagt tcaatagcgc gctcagaaaa aagactttct
     4081 acaatgtaat ccttagccgc aggaccggaa atatattccg tggcgccggc ctggacacat
     4141 aaatccacta aacgctccat cttgccatca agcaaagtgt agtcccagga attactgatc
     4201 accgtttcaa tatttaaata attgcaaata gcctcgatga atcggcgatt caataacgat
     4261 aaatttgtaa aaggcccttc aaaatacagt gactcaagcc aaaccgcgat ttcattaaaa
     4321 taggcggctc gacggtagtt ctgtaccaag gccttccaat gcgtcaatgc ccaatccgaa
     4381 ccgtcgattt ctgtgtcttt tatcttttga tggtatttcc ccttcacctg caccggaacc
     4441 gtcaaccact gcagtccctg gggggtcttg atctgattgc gattgcgcca atcacgccgg
     4501 gtatactgca tatcatcgta aataatgagt tcatccacag cagcgatgag gtcgaagtaa
     4561 cctttccagg gaatgtaatt agattggaga atagccactt ttttcactgc acatcacttc
     4621 ttgccttgta aatctcgtaa gcacgattgg gtgaatcagg aacgcggtca aagttatcaa
     4681 ggatataccg atagatccta ggatgagttc gctgaaaacg caattcatca tgaccatcta
     4741 ggggaaaatc gaaaaccaat gcaaacccag gttttgccgc ttttatacgt tcaatttcag
     4801 cttgctcgaa gtccggggag cgtaaaaaaa acgcataaat ctcccacagg ggagacttcc
     4861 gctcaaacag agcataggcc cctggccaaa aaggggtggc gataaaactt tggccattgg
     4921 gagcgtattg atgaactaat ttacggagca gccccacgtc ctctgcactc tctggatcaa
     4981 tctgaagatg actgccagaa atttccacat ccacacagtg tttatcaatt tgacattgcc
     5041 accctggatg aaacacatgc atgacccaga gactcgcgcc acacagcgca agccctaagg
     5101 gccatttgat tttgccgggt tgatcaacca acagaactaa acaccccacc aaaagaggaa
     5161 aaatcccctg cgctaggtgc cccacatccg cccgtgagta tgcataatgg gcatagggaa
     5221 gcgccaaaaa ggccgttgcc accagcactg gtgaaacttg cttatgctgg atttttttcc
     5281 tgaccaccca aaggatcgat aaaacaccaa agacgacaac acctataaaa aacagaccca
     5341 cgaagacccc tcgcaaggct tgatcaagag gcagcaaatc aaaatgaatc gtccaaggcc
     5401 atggaactgg gagaggaaga ttggtggcac gaacctcaaa caaaaaacgg atactttccc
     5461 aaaatgcagc cgcaaaacct ggcatcaaca gtgccataaa cagaatgggg gaaaaaccaa
     5521 cgacaactcc agccagccaa agcgcagatc cttttataac ttccgaccct ttatctgttt
     5581 tgagactgag ccacgccaag ctcccgagac tgccaacaaa accatagacc ccatgattac
     5641 gcccaaaaat agctaccaaa cctaaacaga gtcctgcaaa aaagtaacgt cgaagtatcg
     5701 gtctctgaat cagaaaagct aaaacaccaa taaacataat agaaagtgaa atatcaaaca
     5761 gcttatgacg tggatacatc cacaccacca aggtcaacgc cgacaaaatc ataaaaaata
     5821 tattttgttt ttttaccgta tgagctatca aaaaaagccc gacaaataag cctattgttt
     5881 ggaaaatcgc cacggaagcc cgtagcgaca taatcccatt atcgcccccc aaactcatga
     5941 atccggcaga ccaatagtaa cgacctggat cataagccat aaaatcccga atcggcactt
     6001 cacctaacat ggtccgctga gctccgtacc aaaggaatcc ctcgtcccat aagttgaagc
     6061 ccatgttgcc ctgccacagg aaaatcatga atacaataaa ggttgctaag cctaaaattt
     6121 gaagaagcaa ggtcttgttt ttcactttac gaaaatactt tctttgtgat ccttaataag
     6181 cacaatccca accgaaccaa tttcaaattg actaactgac tatacttttc actcagttca
     6241 gccaatgatt tacgcagttg aagagagctc tctctttcag ttgaaatagc agccaaaata
     6301 aaacgactgt ctcgagtaaa agcgcttcca ccgcttgcca aattccaggc aagatactct
     6361 tcgagagggg ttttcatggg acctcttccc attccaaatt tttcatcttg ggtcttcagc
     6421 tctatagaaa catccaaagc caaatctcca ctcacttcca agagaccaca actaaaaaag
     6481 tagaattctt taagagaacc gaacttctca caaaggatgg tgtggatatt ctgatcaaac
     6541 tggagaatac atcgcaaaat gaaccagctc ttacgaaagc tctctttata aaaccattca
     6601 ggttttataa ttgcatattt tccgtcttgt cccacgataa tattctgtgg cactaaatcc
     6661 caggcctctc cct
//
LOCUS       DATBTE010000282         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_583452, whole genome shotgun sequence.
ACCESSION   DATBTE010000282 DATBTE010000000
VERSION     DATBTE010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583452"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VIG33_10335"
     CDS             complement(<1..212)
                     /locus_tag="VIG33_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271274.1"
                     /translation="MSLKKLFILTMCHLFCQYTFAGFVVGNGGDAILCQKNSDNSLEG
                     FYSLDYILTLADKKGDDGLTPVTSWN"
     gene            complement(209..1063)
                     /locus_tag="VIG33_10340"
     CDS             complement(209..1063)
                     /locus_tag="VIG33_10340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4423 domain-containing protein"
                     /protein_id="HEY8271275.1"
                     /translation="MLQQKDYSLSMPDAPSLLRAELLLRLKKNPSFSLRAFALNLKIS
                     PGYLSQVLNGQKQLTEEKAYSLVSHLPWSEEKKRLFINLVRWQRSKDLKFKEHLLAEI
                     QKESDSSAIFFDLQIDEFKLISQWHHAAIVELTSIKSFSLTAQNVSQRLGLSTAEAQE
                     AIQRLLRLGILEERQGHLKKTKVNYQIKDIPSEAIRSYHSQNLSKAQEALHSQPLEKR
                     EFYSTTMAINPAKIKKAKALIHKFSTEIMECLEEGPREHLYQFNVQLFQLDCSVRNKN
                     PNLKKEKA"
     gene            1235..1579
                     /locus_tag="VIG33_10345"
     CDS             1235..1579
                     /locus_tag="VIG33_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271276.1"
                     /translation="MKIFILVMTLFLNLNFWAQAIELPREMQRSMEQRILSDLKIREK
                     RLGCHVADIKTTFSKVRVNSRKEHMAEYQVDFRELCAKGISTQFSVKKKVIFGKRGAI
                     LSLSPVEQELKF"
     gene            complement(1615..2367)
                     /locus_tag="VIG33_10350"
     CDS             complement(1615..2367)
                     /locus_tag="VIG33_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY8271277.1"
                     /translation="MGAKTDVIVKMVLVFFISLLSFSIGTFVGKKYSDNQHKLAALEP
                     EKGEQGAREVASQGEQGDAAKEHGTAKVGEGMSDEEIAKLAAEFVADDSTATGSAASG
                     GQEEHGNAEESMASAASKSRSLEKAEPLSAAKNIVHGKAISATAVDEKKLEKENRIPS
                     SIPKDVNQYSAGKFTVQVGAYAKEDEAQKIAGDLKSRGFSAFYIPANVKGQTWYRVSV
                     GLFATPKEAQDYRTELMAKAKLSSALVQKIIQ"
     gene            2429..>2665
                     /locus_tag="VIG33_10355"
     CDS             2429..>2665
                     /locus_tag="VIG33_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271278.1"
                     /translation="MLTSGEKSVNKTASPGKVKAVGILQENSGNSIDLEHALQVGLKA
                     CRLGREVLLKYIGKLHHISEKFEAGLVSEADKESE"
BASE COUNT          732 a          527 c          612 g          794 t
ORIGIN      
        1 ttattccagc ttgtgaccgg agtcaggcca tcatcacctt ttttatcagc cagggtgagg
       61 atgtaatcaa gagaataaaa gccttctaaa gagttatcag aatttttttg gcaaagaata
      121 gcatctccgc cattccccac cacaaaaccc gcgaacgtgt attggcaaaa taaatggcac
      181 attgtaagga taaaaagttt ttttaagctc atgctttttc ctttttcaaa ttaggatttt
      241 tatttctgac gctgcagtcg agctggaaga gttggacatt aaactgatag agatgttcac
      301 gggggccttc ttcaaggcat tccatgattt cagtactgaa cttgtgaatg agggctttgg
      361 cttttttaat tttggcaggg ttaatcgcca tggttgtaga gtaaaattca cgcttttcca
      421 gcggctggct gtgaagtgct tcctgggcct tgctcaggtt ctgtgaatga taagaacgta
      481 tggcttcgga aggaatatct ttaatttgat agtttacttt ggtttttttc aggtggcctt
      541 ggcgctcctc taaaattccc aagcgcaaaa gccgttgaat ggcctcctga gcttcggcgg
      601 ttgaaagacc caagcgttgc gacacatttt gagcggtcag cgaaaaactt ttgatgctgg
      661 tcaactccac aatggcggca tgatgccatt gtgaaataag tttaaactcg tcaatttgta
      721 aatcgaaaaa aatcgcggag gagtcagact ctttctgaat ttctgctagc aagtgttctt
      781 taaactttaa atctttggaa cgctgccagc gtacaagatt gataaagagc cgtttttttt
      841 cttcactcca gggaagatgg ctgacaagtg aataggcttt ctcttcggtg agctgcttct
      901 ggccattgag aacctggcta aggtagccgg gtgaaatttt taagttcaag gcaaaagccc
      961 gcaaagaaaa agaaggattc ttttttaagc gaaggagcag ctcagctctt aataaagagg
     1021 gagcatctgg catagacaaa gaataatcct tttgttggag cattgttttc tccagaaaac
     1081 ggtgcgtttg ctgaggactt tgagtacata tctaagaaaa acaagcctat caagtgtttt
     1141 ctatagcaat cagtttatga ggatgcccag cagtttgagt tggaccaagg ctcctgaatt
     1201 caaccatatt tgggaaattc acgaggggat gcttatgaaa atattcattt tggttatgac
     1261 tttatttttg aatttaaatt tctgggcaca agcaatagag ctgcctcgtg aaatgcagag
     1321 aagtatggaa cagcgaattc tcagcgattt aaaaatacgt gagaaaagat taggatgcca
     1381 tgtcgcagat attaaaacga ccttttccaa ggtgagggtg aattctcgaa aagagcatat
     1441 ggcagagtat caagtggact ttagagaact ttgcgctaaa ggaatctcga cccagttttc
     1501 ggtaaaaaag aaagttatct ttggaaaaag gggggctatt ttgtctcttt ctccagtgga
     1561 acaagaattg aagttttaaa aaaaatgccc ctaaggaagg ggcttgataa agatttactg
     1621 aataattttt tgcacaagag cggagctcaa cttcgctttt gccatcaatt ctgttctata
     1681 atcctgtgct tcttttggag ttgcgaaaag gccaacacta acgcggtacc aggtttgacc
     1741 tttaacattc gctggaatat agaacgcgct gaaacctctt gattttaaat caccggcgat
     1801 tttttgagct tcatcttctt tcgcatacgc tcccacttga accgtgaatt ttcccgccga
     1861 gtattgattt acatctttag gaatacttga aggaatgcga ttttctttct cgagtttttt
     1921 ttcatcaacg gcagtggcgg aaatggcttt tccgtgaacg atgtttttag ccgcagacaa
     1981 aggttcagct ttttctagag accggctttt ggatgctgca gaggccatgg attcttccgc
     2041 gttgccatgt tcctcttgac ctcctgaagc ggcactgcca gttgcggtgc tgtcgtcagc
     2101 gacgaactct gcggcaagct tcgcaatttc ttcatcactc atgccttcgc cgacctttgc
     2161 cgtgccgtgt tctttagcag cgtcgccctg ttcaccttgg gaggccactt cgcgagcgcc
     2221 ctgttcgcct ttttctggct ctaaagcggc aagtttgtgt tgattgtcgc tatatttttt
     2281 gccgacaaaa gtaccaatag aaaacgacaa aagagagata aaaaacacca gaaccatttt
     2341 tacaatcaca tcagttttag cgcccatcta tgaatcccct cggggtgact taaagttgaa
     2401 ttggagtcat tccattgact cttcttttat gttaacgtca ggcgaaaagt cggtcaataa
     2461 aacggccagt ccaggaaagg taaaggcagt ggggatatta caggagaata gcggcaattc
     2521 aatagattta gagcatgcgc ttcaggtggg acttaaggct tgtcgtctcg gtagagaggt
     2581 tctactcaaa tatattggta aactacatca catttctgaa aaattcgaag ctggtttagt
     2641 cagcgaggct gacaaggaat ctgaa
//
LOCUS       DATBTE010000283        41430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1882232, whole genome shotgun sequence.
ACCESSION   DATBTE010000283 DATBTE010000000
VERSION     DATBTE010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 41430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41430
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1882232"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..773
                     /locus_tag="VIG33_10360"
     CDS             300..773
                     /locus_tag="VIG33_10360"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEY8271279.1"
                     /translation="MKLVKVILLIGVVCTLGNLQKLSASETFDSSLAEEAQLTVSFRQ
                     KLERVTWKLISISDQSSRKNLSHYNQTLLFFGKGVWTQKFSGRAACNNFSGEFDDHES
                     AISNVRVRSTRMLCEAYEVEVAYLEALRHALSYRVKGERLLIQSQNGILLTFARL"
     gene            complement(791..3118)
                     /locus_tag="VIG33_10365"
     CDS             complement(791..3118)
                     /locus_tag="VIG33_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY8271280.1"
                     /translation="MQELIALDWIEILEKIRGYITSESAKSKILEMAPLATPELAQQS
                     FQEIAEAAAVLGEGVRPYMQSLDLFPTWFPRLKKKAILKTLEIKDIRGFCLEVLALQE
                     ALSNLNNNWSQKIGSQLMTAEEPLSAIDQILTSSGEIRSDASEKLYNLFREKEKLARE
                     IQTSLDRLVKDHQMETNLQDKYVTTREGRWVLPVRGGMQHYVPGVIHGSSQTKQTVFI
                     EPEKVIPLNNRLRQIEVDIEDEVERLLLELSHYLSSKTEDFQGSFELLEEADLRLAQA
                     QFATMIKGSAVEFTAEDLELLEVRHPLFQLSGKSVVANTVLLNSQKSILLLSGPNAGG
                     KTVLLKSIGLAAQMARCGLPICASESCRIPFFKEILIGIGDAQSVDEELSTFAAHLKI
                     LTKAALMKGPDHLILIDEICGSTDPEEGSGLGRSFIETFSKNNVYAVITSHLGPLKAG
                     WTEKDHVLNGSLEYDPKSGRPTYQFLSGIPGDSLAIQTAKRVGVAKEIIERATELLSP
                     TTRARLQGLEQIEQLKSDIALLQEHLRSETKKATATKNKYETLLNQFNKEKEEYLAKT
                     LKKAEKKVDEAIGFAKAGDIFERHRNLQDIKYKLPEIVKAKPINTGAIESPEDFAKKF
                     PAGSKVFVPSLNQDGIVQSTPNTKGEVMILANSIRLQLPWAELRSPGKPQNPTAQLVR
                     KSSSFTVALNEDETVLDLRGKTVEEAIETLEVALDKAAQIRDDRLKIIHGHGTEALKK
                     AVRTYLSRSIYVKKWKAGAPESGGDGITWVEFNLD"
     gene            complement(3096..4703)
                     /gene="lnt"
                     /locus_tag="VIG33_10370"
     CDS             complement(3096..4703)
                     /gene="lnt"
                     /locus_tag="VIG33_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY8271281.1"
                     /translation="MSKIKMFFKHYSPRNYLWPLLSGFLIGTSYIPLPPWALLFGYAP
                     LWLFLCHRAKTWQEGFAAGWWTQFTLTLIGFHWIAYVAKEFGGFPLPVALLTLLLFAA
                     LAHLYIPLTTALVVFLKEKYSWSLSKTLAASALLLSIAERIWPSLFQWHLGYTLFWIK
                     APAYNWADVIGFEGLSSILLLLNAWLAWLWEHRHQRRAWQKHSLLFVSFLTFFNLSGY
                     FHGQAWNDFDRKLKISIIQANIGNEEKVYAEKGQGFQDEILHKFLQLTRQAVAEAPNT
                     ELIVWPETAFPDYLDRWNSHARIPLLLAAGLQPLRKTLLTGAYAKDAPGEKSPRLAYN
                     ALFLVDANGHQLGKPYHKTHLLAFGEYLPFSERFPILLKLLPFISNFGRGQGPEILIY
                     PHPEGEIKFGAQVCYEGLYPEFTRGLAEKQADILANVTNDSWFGWPFEPRQHMLMTLA
                     RAIEVRRPLIRSTNTGISTVVLANGDVLQHSPLHKEWQGSYDLQLKKNAPTTNFVLWG
                     HFDWILILLALIGLFVKGVLDARTHRS"
     gene            4732..5532
                     /locus_tag="VIG33_10375"
     CDS             4732..5532
                     /locus_tag="VIG33_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271282.1"
                     /translation="MEKLRLRCPLCAKLYEVSEADIKSNSPQFDCVSCATRFGFQYEG
                     PTLRHIETFVVDVSHNNKPDNKIDSKFESKVQSKTCPKCGAINENKTGKKMDECYSCH
                     VIFSRVEELPSDPTLKAQPSLVRKWKNLIMNFNNKELHEDFLKSCYQLDALRFAILKY
                     EELKSAQGGADEISERMIFKAHGLLKVTLSSKTEGEPHFQKEARSARQTWHKYVLWVP
                     LGVSILMILIGFFSLSLRNLVGAGVSVMLLTFGLILFWKGRISLSDFH"
     gene            complement(5558..6904)
                     /locus_tag="VIG33_10380"
     CDS             complement(5558..6904)
                     /locus_tag="VIG33_10380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY8271283.1"
                     /translation="MRSHKLSVFIGGVFVLFIGVFGTYQHLQSDSPTRSSTSLKKSGR
                     LEESKITIKTDPIENEPSVLFNDPAIHQAWGLKKSDAARAWSVSKGSHKVIVAIIDTG
                     ADIKHEDLDSNIWTNPGEAGLDSKGRNKASNGIDDDGNGFIDDVHGWNFVSNNGTLAD
                     NHGHGTHIACIVGAKAGNNKGISGISPEVSLMILKYYDPKIPNTDNLKNTVAAIKYAV
                     KMGANIINYSGGGTEYSKEEHDAIVEAEKKGILFVAAAGNERSNSDQFKYYPADYGLS
                     NIVSITAIDPSTEVLSSSNYGIETVDIAAPGQNILSCLPGNAYGYLTGTSQATAFVTG
                     AAVLVMARREAFKAVDIKKYILSTGDAQVSLVAKTRTSRQLNLYKALTILDQGLGLSG
                     VAAANMGKSSNEAFSVDPNVGKPKDTTSEMTSFGKALIKNMEKVGRVGEKQNSPTN"
     gene            complement(7028..9163)
                     /locus_tag="VIG33_10385"
     CDS             complement(7028..9163)
                     /locus_tag="VIG33_10385"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY8271284.1"
                     /translation="MNFIAAAVFANLLLGCTTTSVKTPSYDFIIQNRNHTLKELETLQ
                     VNSENQIWWRKYRQGLLSLEKNPELACSQFTILAKDLDFPLKEIALLRAHQACADTAG
                     LPSLNLELYRTSYKWSQDVLADVLFKTARKTEDKNDDLVALKERARHETNPKKKEQYL
                     VEALKIAEAVKSASEIETVQSHLYHSFPRLKPNLTLKDLPAAAMDFRQHREFEKALAL
                     YKKILKSPQASEDDKFQALKNIRMTYKVAQNKNAYIEATTQLVNSTKAQYKADKKNSQ
                     TLKHLHESYILLVRTLWTEDKLNLALQYLNEAQRQLKGVYPLDEIYFILGRIAEEKGD
                     FAKASDYYEASLKEPLSSSNIRERVLWLHPWVLYKMKKYEAAAAKLQEFSQKAKDNSD
                     KMRSSFWHARALKNLNKPEEAKILLQQIIKEDPIGYYGMVAVRELGQSFSAIKSTEKE
                     FPYSLFNLKEIGPLSALQTEWLMAVGENTFSEKIIDQLSQDLRQQGRNDEQSWLIVLT
                     SYARANLYLPLFAAFNTLPLEVKDKMVQKHPELLFPRDYKDLIIQAAQTEQIPPELGF
                     SIIRQESAFNSRARSPVDAFGLMQLLPSLAKELSHNTKVPYQEAEDLYDPEINVPLGT
                     KALKNLLMQYDQRYILAVSAYNASGSAIRGWLKTRYREDALEFIEEIPYDETRTYIKL
                     VMRNFVLYKRFNQTEESIVFPEEWLKLAK"
     gene            complement(9163..10062)
                     /gene="htpX"
                     /locus_tag="VIG33_10390"
     CDS             complement(9163..10062)
                     /gene="htpX"
                     /locus_tag="VIG33_10390"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEY8271285.1"
                     /translation="MKFFRRFGYFILTNLLVMVTIGIAWSLISHFLNLAGFNQYLPSL
                     MAFCLVWGMGGAFISLLMSKFAAKHFYRVQIIDPNTQVPDLRQLVEKVHELARRAQLP
                     KMPEVGIYESYDVNAFATGPSKKNSLVAVSTGLLQRMNDKEIEGVLGHEISHISNGDM
                     VTMTLIQGVVNAFAMFFSRLLANVLASNVDEKYRGIVHFICVLIGDIAFTLLGSIVVN
                     FYSRKREFRADAGSAKVSSPGNMIAALQRLQSLHEAPPSRDDAMATLKISGREKKGSI
                     ADLFMTHPALEDRIRALERGRIA"
     gene            complement(10126..12483)
                     /gene="clpA"
                     /locus_tag="VIG33_10395"
     CDS             complement(10126..12483)
                     /gene="clpA"
                     /locus_tag="VIG33_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEY8271286.1"
                     /translation="MMTRELERRLSVATELAKKAYHEFVTLEHILLALTESPLMVEII
                     QACGANVQKLKLDLKDYLKKTAPHITQEQLDSYGGFESWTPEFTLACHRLLQRAAIQV
                     KSSGRNQINEGSLLVALFYEQDSHAVFALSQQGLSQFDVISYLSHGVAKDVSGTAEKN
                     SASPAPRMDIDGQPADESKNSPLESFCTNLNEKAKAGKIDPLVGREDVIERCVQILSR
                     RTKNNPLLIGEPGVGKTALAEGLAAKIVHGEVPEKLKTAVIYSLDVGTLLAGTKFRGD
                     FEGRLKAVIKEIEKRPNAILFIDEIHNIVGAGATSGGSLDASNLLKPALANGDISCIG
                     STTHTEYRQYFEKDRALNRRFQRIDVNEPTAADCVAILKGLRKSYEEFHNVRYTDEAL
                     KAAVDLSIKHIHGKLLPDKAIDVMDEAGAYFRLRQNTSAENAQSKITINLQAIEEVIA
                     KITGLPIANISSNEKIQLRDLDKKLKALIYGQDEAIDRLVTSIKFARSGLSRPDKPIG
                     SYLFTGPTGVGKTEVCKQLAHILGIHFHRFDMSEYMEKHAVARLIGAPPGYVGYEEGG
                     LLTEAVNKNPYAVLLLDELEKAHPDVTNALLQVMDAGRLTDSHGRIADFKNVIFVMTS
                     NAGAMEVARGSIGIVEENRSMQSMEAIKKAFSPEFINRLDAVVSFKDLSEDIILKVVA
                     KFVNELKMTLAEKKVELLVTQEVLKWLMKKGYDKVYGARPLARTVDEHLKKPLVDELL
                     FGRLVDGGRVSVEVEGTVLKFQFSTTQNDGPNRTGSKKPKETITT"
     gene            complement(12502..12867)
                     /gene="clpS"
                     /locus_tag="VIG33_10400"
     CDS             complement(12502..12867)
                     /gene="clpS"
                     /locus_tag="VIG33_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEY8271287.1"
                     /translation="MSKINGNSSGPNSNDTPKNFPFISTEGETSIQTIPRIDTPKMYK
                     VILLNDDYTPMEFVVLILRRFFGKSEEEATKIMIDVHKKGAGAAGVFTLEVAEMKVMQ
                     VNQYSQMNEHPLKSTLEEE"
     gene            13091..13669
                     /locus_tag="VIG33_10405"
     CDS             13091..13669
                     /locus_tag="VIG33_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY8271288.1"
                     /translation="MKDVGVNRNQAGFSLVELMVVVAIIGILASLAIPSVGKYMAKAR
                     QSEAKTQLASLYTAEKAFYSEYTQYDSRFGAIGYSPEGKLRYNVGFAAAVASTTYTGT
                     LAGNQLLFNTLTYCPATTGAAALGVTGCGILPGATNAPPPAINAASTVANPAGASTFI
                     AEATAVVHSQGLADRWTVNESKNMLNPQNGVP"
     gene            13674..14345
                     /locus_tag="VIG33_10410"
     CDS             13674..14345
                     /locus_tag="VIG33_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271289.1"
                     /translation="MSKWLDFTKSSSFSLLGLFSFFLIMYSHIYASKGLLEKPRDLLS
                     PPAGVEHFTFGHRDVTADFLWIRAIQDFDYCDQQIAKNLCVSKGWLYRMLNTITDLSP
                     TFRMPYATGAVALSVLVSDIEGAARIFDKGIENYPKDWRILYRAAYHYLYEVKNKKKA
                     ADLFIRAGREGAPAWVFSLAGGLYNDSNERAAAEAVLQEMLKTEMEPIIIKRLQEKLE
                     KIRTL"
     gene            15158..16264
                     /gene="dnaJ"
                     /locus_tag="VIG33_10415"
     CDS             15158..16264
                     /gene="dnaJ"
                     /locus_tag="VIG33_10415"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY8271290.1"
                     /translation="MLSQRDCYEILGVARDADADTIKKAYRKLAMQFHPDKNPGNPAA
                     EEKFKEAAGAYEILSDAQKRAQYDRFGHAAFSGGGRGQQDVNDIFANFGDIFGDFFGG
                     GGQQRSRRSRHEPRRGADLRYLTEISLKDVLNGLEKDIEFDTDKSCDECHGTGAEKGS
                     APVTCPTCHGSGQVVRSQGFFSVASTCPTCRGEGSTIKNPCKACKGKGRVSEHRKILI
                     NIPPGVDTGTRLRVGNEGEGGFNGGPAGDLYIEIRVKDHPRFEREGEDLHSTVSVPYL
                     QLLLGAEIKVETLSEPAILDIPKGTANGKTLKLAGQGLPSLRGQRRGDLYCTLEVEFP
                     EKISKEEEKILRELAKLKGIEVKGESGGLFGRKK"
     gene            16355..18274
                     /gene="dnaK"
                     /locus_tag="VIG33_10420"
     CDS             16355..18274
                     /gene="dnaK"
                     /locus_tag="VIG33_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEY8271291.1"
                     /translation="MGKIIGIDLGTTNSCVAIMEGGEPKVLVNEEGARTTPSVVAFTK
                     DGERLVGQIAKRQAVTNPENTIYSAKRFIGRRFEEVTEEIKLVPYTVVAKGNDCAFKL
                     SNSKTVSPEEIGAAVLAKLKKVAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRIAG
                     LDVKRIINEPTAAALAYGLDRKKDEKIVIYDFGGGTFDVSILEVGDGVVEVRSTNGDT
                     HLGGDNFDTVILEWLIAEFKKDQGIDLKSDKMALQRLKEAAEKAKIELSSAQDTEINL
                     PFITADQSGPKHLQTRLSRAKFDQMTENLVKRSMEPCRKALEDAGLKSSEIDEIVLVG
                     GSTRIPAIQKAVKDFFGKEPNRTVNPDEVVAVGAAVQGGVLAGEVKDVLLLDVIPLSL
                     GIETLGGVMTTLIERNSAIPTKKTQVFSTAADNQPAVDIHVLQGERKMSTDNKTLGRF
                     ELTGIPPAPRGVPQVEVTFDIDANGILSVSAKDLSSGKHHQIKITAQSGLTEEEVKKA
                     VREAELQADADKQKAEAVQAHNNLDNLIYQTEKVIKDSGAQIPESEVKSANETVAEAK
                     KVLENKSATVTELKAAFDKLQAVTHKLSSELYKKQGGEAGGGASGDGASGAKESEGAA
                     KGNDDVIDADYKDVN"
     gene            18386..20278
                     /gene="acs"
                     /locus_tag="VIG33_10425"
     CDS             18386..20278
                     /gene="acs"
                     /locus_tag="VIG33_10425"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEY8271292.1"
                     /translation="MNFDQYKKMYEDSIRNPEEFWGKQADRLHWFKKWNKVKDTSFTS
                     PVSIKWFEGGELNVSYNCIDRHLADNRADKVALIWEADDPHIPAKKVTYKELHQNVCK
                     FANILKCNGVKRGDRVTIYMPMILETSYAMLACSRIGAIHSVIFGGFAPDSIADRILD
                     CESDFVITADEGFRGSKIIPLKKNVDQALKRTPKVRKVLVIKHSGNAVNFEKDRDLWY
                     HEEILQVDAQCEPERMNAEDPLFLLYTSGSTGKPKAVLHTTGGYLLYASLTHKYVFDL
                     RENDIFWCTADVGWVTGHSYLIYGPLANAATTLMFEGVPNYPNFSRFWQVVDKHQVTI
                     FYTAPTAIRALMREGDEHVKKCSRQSLRLLGSVGEPINPEAWHWYHNVVGDQRCAIVD
                     TWWQTETGGILISPLPGAMELKPGSATLPFFGIKPVVLTPEGQRLEGVCEGVLALEDS
                     WPGQMRTVYKDHQRFEHTYFASYKGYYFTGDGCRRDEDGYYWITGRVDDVLNVSGHRI
                     GTAEIESALVGHAGVSEAAVVGYPHDIKGTGVYAFVTLRTGLEGSEEIKKELGQWVRK
                     EIGAIAAPDLIQWASSGLPKTRSGKIIRRILRKIAENHPDQLGDISTLADPSVVEDLV
                     KNRLQK"
     gene            complement(20288..20848)
                     /locus_tag="VIG33_10430"
     CDS             complement(20288..20848)
                     /locus_tag="VIG33_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8271293.1"
                     /translation="MTEIYRNIAWKLINLRQREGLTQEQLSEKISLSKCQISRAERGQ
                     GQITLESLFILSTYFEVPLEDLFSRTSKMDIEMKLRTEYVERKLNTPCINIGDNLAIK
                     NYVKAFENTAKIRSIEIASKSIRKMKVEKNFRYEIYVLQGSFIARTNDETFEIKKDQL
                     LSVKGTGTLKLVNMHSERTTVLTIAY"
     gene            20972..22213
                     /locus_tag="VIG33_10435"
     CDS             20972..22213
                     /locus_tag="VIG33_10435"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HEY8271294.1"
                     /translation="MNLKIQQSLDQALVAQVYKKEVQRGLGVGTPCLEGGSRVFRGDM
                     RNAPGLSKPILNSQVNFPLCLAPMVGLSHVALRLTVREYIPQGAKTIWPTEMLNSRRL
                     PQENFATNPHTLRGPQETELVPQILGNEEKAISESVKKLIYEWGAEGIDINMGCPVQK
                     ALRHNYGVALMGDPEYAARIVEMTVKNSTVPVSVKLRAAPQPGKDSESLNTSGNYSGN
                     DFGYLHNFVQRLKEAGASWVCLHPRTAAQKRRGVADWSQITKLREAVDLPLIGNGDVQ
                     TAEDALHMLQETGCDMAMAGRALAARPWMMWQFGEMLGFAPPVGREGQRAPQGPLEEG
                     AEYGKALLALIEHCAQYFTEDMALRKIRFHVRTTEVWLDFGQSLVGVCDKANNLKDLR
                     ISVKNFFQAPIEMCARTELRQ"
     gene            complement(22232..22498)
                     /locus_tag="VIG33_10440"
     CDS             complement(22232..22498)
                     /locus_tag="VIG33_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEY8271295.1"
                     /translation="MKNQKAKVVIYTKDPCPYCVRAIRFFEEYGIQYEEVDLTDKPEE
                     IDRIKSETGWRTVPIIMVNGVLIGGYNDMKALHEEGKLETLLQA"
     gene            complement(22531..23472)
                     /locus_tag="VIG33_10445"
     CDS             complement(22531..23472)
                     /locus_tag="VIG33_10445"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HEY8271296.1"
                     /translation="MRLLLAPMEGVVDWVMRDTLSQIGGIDQFVTEFLRVTDKLHPEA
                     VIYRNCPELKTGSRTRQGTPVFVQLLGGQAEPLALNALRAVKLGALGIDLNFGCPAKT
                     VNRHDGGASLLKSCDRLYTIVKTVRDAVPSAIPVTAKIRLGFEDPSVCMENARAIEEA
                     GATWLTVHCRTKTDSYKPPAYWEWIPRIRETTKIPLIANGEIWNVSDFLRCREITGCN
                     DFMIGRGALSNPYIFKQIRGTNKSVTDPWPNTKHLLPGFFEASTFYINEYFAVSRTKQ
                     WLRALSLKNSEAKAIFDQLKVLKKPLDFRSQLEILCS"
     gene            complement(23473..23931)
                     /locus_tag="VIG33_10450"
     CDS             complement(23473..23931)
                     /locus_tag="VIG33_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEY8271297.1"
                     /translation="MNYWLMKSEPDVYSLDHLERDKTTWWEGVRNYQARNFMMKEMKI
                     GDLVLFYHSNAEPSGIAGIARVSSLAQPDKTQFNKKSEYYDEKATKEKPQWFCVQVEY
                     VKKFKQLISLAELRQVNSLAEMLVLQKGSRLSVQPVKEEHFNIVKKLGEH"
     gene            24044..24820
                     /locus_tag="VIG33_10455"
     CDS             24044..24820
                     /locus_tag="VIG33_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY8271298.1"
                     /translation="MNTKWAIITGATAGIGWATAEEFAAAGYSLILIGRRGNRLKELK
                     DHLLKNFVQSGKSQEFKTFPLDVTKKSQIDDFVKAEGLSLEKVQVLVNNAGLAKGTEK
                     VQEARISDWDQMIDTNVKGLLYLTRAVLPSMVKRKSGNIVNLGSVAGRLVYPGGAVYC
                     ATKFAVRAISEGLRMDLAGSNIRVTNIEPGMVQSEFSLVRFDNPEKADKVYEGMTPLS
                     PKDIAETILWCVQRPSHVNIQELVIYPTDQASVGQVVRRA"
     gene            24817..26211
                     /gene="fumC"
                     /locus_tag="VIG33_10460"
     CDS             24817..26211
                     /gene="fumC"
                     /locus_tag="VIG33_10460"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEY8271299.1"
                     /translation="MMDFRIEKDTMGEVKVPADKFWGAQTQRSRENFKIGGDTFPREM
                     IKALGVLKKCAALTNAKLGLLDAKKCEFIVKAADEVIAGNLDAHFPLVVWQTGSGTQT
                     NMNANEVIANRAMNMQGLQLPNKEIHPNDDVNKGQSSNDTFPTAMHIAVAHQVHHRLL
                     PMLEKLHGALVKKQEEFKDVVKIGRTHLMDATPLTLGQEFSGYATQLKYSIQRVKNTL
                     PHLHQLALGGTAVGTGLNTHIQFPGEAARTIAAETKIPFVSAENKFEALAAHDALVEV
                     SGALNSIAVSLMKVANDIRLLGSGPRCGIGELQLPENEPGSSIMPGKVNPTQSEALTM
                     VCAQVMGNNVAVSVGGANGHFELNVFKPLIVFNVLNSIRLLADGCESFTDHCVSGIEA
                     NRQQIKKHLDNSLMLVTALNPHIGYDNAAKIAKTAHKNGTTLKEEAVKLGLLSAEQFD
                     KIVRPEDMVHPLGK"
     gene            26227..27201
                     /locus_tag="VIG33_10465"
     CDS             26227..27201
                     /locus_tag="VIG33_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271300.1"
                     /translation="MANVNANASVKLNPLLQKASWMESGDIKPYVFVRPENQIANPGT
                     RHKLDWFSHGSVYKDPLDIVEVNFADRIYSIEERAFGPSNMAMPRWVFYDCAVIPGFV
                     AGFAARPSALKKEVRDILDPVKFPATNSPIFKSSLAEAQAMIVKSLDDMDWVPLSLFI
                     IIPTMHRGEWVAHNLCSVNSLLAKEHQFYGLGFLSKAFGLWYANVEQCSGMTQWGSPA
                     LKLHSHFGHLEIVGAYAPVHSHAKTVTYRTQVNTVCWEQFFSREQDLAFLENYGPSDS
                     KIDPKNEQSMLDLQKKIEKEEGPFYLNAMEIAAKNLDESLTIYKPKGY"
     gene            27203..27640
                     /locus_tag="VIG33_10470"
     CDS             27203..27640
                     /locus_tag="VIG33_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271301.1"
                     /translation="MPVNQLPPVAKSFFTFCKKCNSDRYHTVLAHTTSRTAKIKCEVC
                     GSQKSYSLPKASSSSTGKPKVTRPRAGSETARRSSHNAEYELLLETYEKSSPVTYSIK
                     GKFTKNQKIEHPKFGLGVIRDAQLERIEVVFADEVRNLIHNRQ"
     gene            27640..29661
                     /locus_tag="VIG33_10475"
     CDS             27640..29661
                     /locus_tag="VIG33_10475"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEY8271302.1"
                     /translation="MNRWTKGLNPEQIKAVEHDFGPLLILAGAGSGKTTVLVSRTGRL
                     IADRIANADEICVLTFTNKAAKELKHRVTSKVGSQAKKGLWAGTFHSFGLQVLRRFHK
                     QVGLSPYFGIVDQSDCNSIIKELMKDLKNSGKDKFDVDKILNMINERRTGMAPKTEAF
                     DEYHEMVEVLTPKFNKKLELLGVVDFEGLLIKPLELFRNHPETLEKIQNSFTQVMVDE
                     FQDTNRLQMELIHQIVKTHNNITAVGDDDQSIYGWRGADVQNILNFPKEFKSCEVIKL
                     ERNYRSSTEILAVANAAIAQNKNRHGKVLRSDMAAETGDLPEVFVLEREEDECDFVVS
                     EVQHFMRQGYGYKDFAVLYRSNTQGGLIESSLRRANIPYTITGGTSIFDRKEVKDVMC
                     YLKQALAPNEVSLRRIINVPPRGIGETTIEKLTEYSKEHKITFLEACQQWKEAGIPEK
                     TGESIDHLLAFCHTLPSQILDYKVGSSPGAHFVEIMQELGYRQFIYNGAGDPTVAEKK
                     WQVVEIVGRILDAYLGRRSYEIENIKSFIDAMMLRDDVNEEDAQQNKMQLMTLHASKG
                     LEFPVVILAGLEEDLLPHKTLGSDIDEERRLFYVGVTRAKKRLVMSRCQQRKRHGVVR
                     PVAPSRFLLEVAQKLYKEYPAGARPVSGQEREDLVSSFLKGLGNKTQSK"
     gene            29747..30529
                     /locus_tag="VIG33_10480"
     CDS             29747..30529
                     /locus_tag="VIG33_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY8271303.1"
                     /translation="MARAKKAFPYKVISVQMLKGGVAKTTSTLNIGLGAASYNARVLF
                     IDMDQQANLTFALGVGVEEAEEKPVWLDIVEKKATIQDCIIKLTDKIDLIPSNLNNSV
                     LDRVLLNSNRNWAQAVKSPLEQISKDYDIILIDTAPALSATNTAVVVASDLVILPVNP
                     DRFSYMGLEKHLEELREIKKDFDLDFSKKILFTKFDAREKVSHELLQKCMDSFEHLLM
                     KSYIRTSTEVKNTIGTGKTIYHTKSNAREDYDLITREVLAIS"
     gene            30542..30994
                     /locus_tag="VIG33_10485"
     CDS             30542..30994
                     /locus_tag="VIG33_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271304.1"
                     /translation="MPTSKEYISKKKKARRRPGREEVSEEISEEDLSEPVASTSTSQS
                     ASESASESFSDSEETPLRKKIHIEFPYSEAVRAKVPKAFDIAENVAEEWVNDGNFENV
                     PLGHPLAQITVAAGLRKAKEVEKKLEEKGVFMMAKMGLEYAKSKLHKK"
     gene            30966..31319
                     /locus_tag="VIG33_10490"
     CDS             30966..31319
                     /locus_tag="VIG33_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEY8271305.1"
                     /translation="MQSQNSIRNNRGPIEALLRERIETAFTPEYYELENESHRHSVPA
                     NSETHFRLLVVSKSFEGKSRLDRSRLVNELMKPLMAQGLHALSQRTLTPPEWLQIKDT
                     FVMVSPECRGGSRQS"
     gene            complement(31336..31581)
                     /locus_tag="VIG33_10495"
     CDS             complement(31336..31581)
                     /locus_tag="VIG33_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271306.1"
                     /translation="MDKPNKDETKPSWDHINTDLKGALDSWTDLSGKVANKVSPEEKQ
                     LRDIKTILVTLKDQLKEFSDDALTIEDNKNKEEEKKA"
     gene            31698..33371
                     /gene="ettA"
                     /locus_tag="VIG33_10500"
     CDS             31698..33371
                     /gene="ettA"
                     /locus_tag="VIG33_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEY8271307.1"
                     /translation="MSQDIIYTMKGVSKVYPPNRFVLKNIYLSYFYGAKIGVLGLNGS
                     GKSSLLKIMAGLDKDFLGEAFPAKQMKVGYLEQEPHLDPSLTVKENIFSGMGELPILM
                     KQFNEISEQFNDPDLDPDKMDKLIEKQGAIQEKLEALGGWEVDQKIEIAMDALRCPEG
                     DLPIGPLSGGEKRRVALCRLLMSDPDILLLDEPTNHLDAESVAWLEQYLAKFPGTVIA
                     VTHDRYFLDNVAGWILELDRGEGIPWKGNYTSWLEQKDNRMAQEQKDNARRNKTLERE
                     LQWIRQGAKARQSKAKSRISAYENLLKEPTPEKLQEMSIYIPPGPRLGDIVVEAKNIT
                     KAYNGRLLFDDVSFTIPRGAVVGVIGPNGVGKTTLFRMITGQEKADSGSFKVGETVKM
                     AYVDQSRETLNADKTVWEELSGGNELIQLGQKEMSSRAYVSWFNFSGSDQQKKVGVLS
                     GGERNRVNMAKILKQGANLLLLDEPTNDLDVNTMRALEEAILEFGGSAIVISHDRWFL
                     DRICTHIMAFEGDSKVEFFNGNFSEYEEDRKKRLGADAAPKRIRFRSIN"
     gene            complement(33368..33778)
                     /locus_tag="VIG33_10505"
     CDS             complement(33368..33778)
                     /locus_tag="VIG33_10505"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY8271308.1"
                     /translation="MGLQASFQQILESKIKIASDPRDLNKSPQQDFEPAHLAYLMGHI
                     RQTFFHPPPQLRVHKIYPSPPRPPHTLSEEQQVAVSFFLFYGASLSPAFPQKELKKAF
                     RALALKLHPDMNKGATSAFIELKNNYEMLNKLFC"
     gene            34165..34548
                     /locus_tag="VIG33_10510"
     CDS             34165..34548
                     /locus_tag="VIG33_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEY8271309.1"
                     /translation="MAKAKKATKKAAPKKTATKKATTKKAAPKAAKAAKAPKAKKAAS
                     KRKPNPAFMRPLTPSTTLAAVVGANPLPRTEVVKKLWAYIKKNNLQDSKNRRNINADD
                     KLKPIFGKNTVSMFEMTKLVSKHLK"
     gene            34635..35399
                     /gene="map"
                     /locus_tag="VIG33_10515"
     CDS             34635..35399
                     /gene="map"
                     /locus_tag="VIG33_10515"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY8271310.1"
                     /translation="MGIKPLSLEEIKKMKLACRIAADTLTYLDKYIKIGMTTNEVDEL
                     TNDYMLTRGAKSACLGYGGYPKYSCVSVNEVICHGVPDETPLKEGDIVNVDVTAFIDG
                     FYGDTSKTYMMGKVSDTARDLVEAARMARDIGIEAIRPKGYTGDIGFETHKFITRKGY
                     TAIREIGGHGIGRVFHSEPFVPAYGKKGKGELLVPFHCITVEPMINQGTNEFIEFDIH
                     GSSIKYYKTADNLLSAQFEHTVLVTDTGYEILTLQS"
     gene            35463..36884
                     /gene="ahcY"
                     /locus_tag="VIG33_10520"
     CDS             35463..36884
                     /gene="ahcY"
                     /locus_tag="VIG33_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY8271311.1"
                     /translation="MSLTTNTGKAAMKKNMKIAAKAVTKNTTPTKTPVVDYMVCKEAM
                     ENPKVFAELARWGREEISIAETEMPGLMALRKEYKKRQPLKGANISGCLHMTIQTAVL
                     IETLVELGAKIRWSSCNIFSTQDHAAVAIAAAGIPVFAWKGETEEEFNWCIEQTITGW
                     GKDGFNMILDDGGDLTNMMHEPRFAKEMKKIIGISEETTTGVHNLELMLKQGRLKVPA
                     ININDSVTKSKFDNLYGCRESLADGIKRATDTMVAGKIVVVAGYGDVGKGSAHSMRGL
                     GARVLITEIDPICALQAAMEGFEVTTMEEAAPFADIFVTATGCCDIITEKHFSKMKNN
                     AIVCNIGHFDIEIDMAWLNKNSKMREIKPQVDIHTMKNGRQIIVLAKGRLVNLGCGTG
                     HPSFVMSNSFTNQVLAQMELFLNHAQYQDIAVYRLPKHLDEKVAALHLEKLGVKLTKL
                     SGKQSKYLHIDTEGPFKPDHYRY"
     gene            complement(36976..37506)
                     /gene="orn"
                     /locus_tag="VIG33_10525"
     CDS             complement(36976..37506)
                     /gene="orn"
                     /locus_tag="VIG33_10525"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEY8271312.1"
                     /translation="MEKLFWIDMEMTGLDVKKEVIIEVAAIITDFDFQELDQLETVVR
                     QPQIYLDNMDNWNKEHHKKSGLTAKVPYGMALDLAEAKLCDLAKKHFPDPRNKPILAG
                     NSINQDRLFIDKYMPEFSERLHYRVVDVSSWKVIMNKKFGITYSKSNQHRALDDVRES
                     IQELKAYCRFIEIPKK"
     gene            complement(37506..37898)
                     /locus_tag="VIG33_10530"
     CDS             complement(37506..37898)
                     /locus_tag="VIG33_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8271313.1"
                     /translation="MKNHKRGRLLIIDDEADLREVLIALLEEHVLEIVQASNGKEGIE
                     ILTHKEFDAVLSDEKMPKKSGLEVLKWMREQGLKTPFIIHSGYRHQDRASEAIRLGAF
                     AFIDKPWDENKLIQTVCDAIDVGLAKKS"
     gene            38215..38291
                     /locus_tag="VIG33_10535"
     tRNA            38215..38291
                     /locus_tag="VIG33_10535"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38249..38251,aa:Asp,seq:gtc)
     gene            complement(38376..39656)
                     /locus_tag="VIG33_10540"
     CDS             complement(38376..39656)
                     /locus_tag="VIG33_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY8271314.1"
                     /translation="MKKKKEEALKNYPGIYKLLDWNEKLKRWQDSGKYRSIRRVRIEG
                     ELSKKESCFFDSLEDAKEFRIGKLAKHETGRHHKNVTLDSKDKLRFAALLQEWKDFHY
                     MTIDFTTRQTYDKKLPPLNYLSSYYVDEISPKVIDQMIRHWHENYPRRKTRFSFEKEL
                     DALKVVLNYYRKRVNALYPLPIFKEHYQAAKVVRRAKQPVRSLRPDDLGNFLRALKAQ
                     KNPLYFPLALTQFGLGLRIGEACGLTWDALDLERSIATIEQTIIWDQESWEPKIKSCP
                     KNGQVRFLVIPEILVEELRLLKKIRSPNTSLVFHSEGRPMIRKSIGCAYNRALKLCGV
                     NYVSGTHLIRKTSATQANRITGDFHAVSLNLGHSSLDETQKYVEEVDEGKWKVARALD
                     GVARAVLALPEKAKECIGGASSTPPVPHCPVKGN"
     gene            complement(39653..39880)
                     /locus_tag="VIG33_10545"
     CDS             complement(39653..39880)
                     /locus_tag="VIG33_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HEY8271315.1"
                     /translation="MRIDSSRVKNNSPSLFEKTNKLVWTIDDVAKELSCSIRHVRKLV
                     SKNRIPYLKVGRLVRFSPQRISEWLHKGGTR"
     gene            complement(39902..40426)
                     /locus_tag="VIG33_10550"
     CDS             complement(39902..40426)
                     /locus_tag="VIG33_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271316.1"
                     /translation="MESLTENIITPTNKPKATERICLGSDEATKAQGWLEQINSSSKG
                     FLALSKSDVVNFLIREFKSELTTKEMQKIKFYHYDPIKHLNWITPRLKEALINGDTEK
                     VAEIQEEIRSIELAVIQHADTKIADRHETMVTPPKTRKKRSKKDMSEAPSNSPESIPL
                     ADINDVESARKLIL"
     gene            complement(40729..>41430)
                     /locus_tag="VIG33_10555"
     CDS             complement(40729..>41430)
                     /locus_tag="VIG33_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271317.1"
                     /translation="YVEAKFNDTEIFWAWQLREKGFESIKLNLPELEEDGYLSENHKH
                     DRHVLAFHLGEWLGRRPERILFFSEQELRRCSKDQYPDWIPKTKDHRPDGYTRVLGLQ
                     KAVTYAIEVELSPKSTSRYENVIQFYRHQRQVDRVLWLVSDPLIMANILRAKDCVKDP
                     SENIHLFVDLKEYMEKGLNAVAINQRSETLFTLRENLRGLLGDFYGDILGTSRELNRV
                     TAHYHPTKVIGKSKT"
BASE COUNT        11058 a         8937 c        10025 g        11410 t
ORIGIN      
        1 aagtctttat tctatccgaa tgttttcatt ggacttctgg ttgtactcaa acagaagtaa
       61 agtcgcgcta cgagtttcaa aatgaataat aagcgccttc gcgacttatg atagccgtat
      121 cttcgtctag tagaaaactt aagacccttc taaaaaacgg agagccattc ctgaccttct
      181 tgagagcttc tttttaatag actgtaataa aaatgtatgt aatttaaagc cagtcagtga
      241 gcggttcggt cggcaattat tttacttctg gaagtaaagt tcttagagga aagaggcata
      301 tgaaattggt gaaagtaatt ttgttaatcg gcgtggtttg taccttagga aatttacaaa
      361 agctgtcggc ttcggaaact tttgacagtt ctctagcgga agaagctcaa ctcacagtca
      421 gcttcaggca aaagctcgaa agagtcactt ggaagttgat ttcaatttca gaccagagct
      481 ctcgaaaaaa cctatcgcat tataatcaaa cactcttgtt ttttggtaaa ggcgtttgga
      541 ctcaaaaatt cagcggtcgc gctgcctgta acaatttcag cggtgaattt gacgatcatg
      601 aaagcgccat aagtaatgtt cgagtgaggt ctacgcgaat gctttgtgaa gcttatgagg
      661 ttgaagtagc ctatttagag gccttaaggc atgcgctgtc ttacagagta aaaggggagc
      721 gtctcttgat tcaaagtcaa aatgggatct tgcttacttt cgctcgcctt taggcatcga
      781 ttttaatctt ttaatccaga ttaaattcca cccaagtgat tccatcacct ccggactcag
      841 gggctccggc tttccacttc tttacataga tactgcgaga aagataagtg cgtacagctt
      901 ttttcaaggc ttcagtgccg tgcccatgaa tgatttttaa gcggtcatca cggatttgag
      961 cggctttgtc tagagccact tccaaagttt cgatagcctc ttccacggtt ttcccgcgta
     1021 aatccaaaac ggtttcatct tcattcaagg ccactgtaaa cgaagagctt tttctcacca
     1081 gttgtgccgt gggattttgg ggctttcctg gagagcgaag ttccgcccaa ggaagttgca
     1141 gacgaatgga atttgccaaa atcatcactt caccttttgt attgggtgtg ctttggacaa
     1201 tgccatcttg atttaatgaa gggacaaaaa cctttgatcc tgctggaaac tttttagcaa
     1261 agtcttctgg ggactcaata gcgcccgtat taatgggttt ggcttttaca atctcaggaa
     1321 gtttgtattt aatgtcttgg aggttgcgat ggcgttcgaa aatatctccg gctttggcaa
     1381 agccgatggc ttcatcgact ttcttttcgg cttttttaag agttttcgcc agatactctt
     1441 ctttttcttt attgaattgg ttcaacaaag tctcgtactt gtttttagtc gcggtggctt
     1501 ttttggtttc gctgcgcaag tgctcttgta aaagcgcgat atcacttttc agttgttcaa
     1561 tttgttcaag cccttgcagg cgagcccggg ttgttggaga caagagttcc gtggcgcgtt
     1621 caataatttc tttagccaca cccactcgtt tcgccgtttg aatagccaga gagtcaccgg
     1681 gaatcccaga aagaaactga taagtcggac gtcctgattt tggatcatat tcgagacttc
     1741 catttaaaac atgatctttt tccgtccatc cggccttgag aggtcctaag tgggaagtaa
     1801 taacagcata gacattattt ttcgagaagg tctcgataaa gctgcgacca aggccgctgc
     1861 cttcctccgg atcagtggat ccgcagattt catcgatcaa aatcaaatga tcgggacctt
     1921 tcataagtgc ggctttagtc agaattttta ggtgggcagc gaacgtgctg agttcttcgt
     1981 ccacgctttg tgcatcccca atcccaatta agatttcttt aaaaaaagga atgcggcagc
     2041 tctcgctggc acagatcggc aggccacagc gagccatttg tgccgccaaa ccaatggatt
     2101 taagcaacac tgttttgcct ccggcattgg ggccggataa aagtagaatg gatttttgcg
     2161 agttaagaag aaccgtattg gcgaccacgg attttccgga aagttggaaa agaggatgtc
     2221 gcacttcgag caactcaaga tcttcggctg taaattccac cgcagaccct ttaatcatgg
     2281 tggcaaattg ggcttgagcg aggcggaggt ccgcttcttc tagaagttcg aagcttcctt
     2341 ggaaatcttc ggttttcgaa gagagataat gggaaagctc cagcaggagt ctttcgactt
     2401 cgtcttcgat atccacctca atttggcgaa ggcgattatt gagaggaatc actttttcag
     2461 gttcgataaa aactgtttgc ttagtctgtg aagacccatg gatgacgccg gggacatagt
     2521 gttgcattcc tccgcggact ggcaataccc aacggccttc gcgggtggtg acatatttgt
     2581 cttgtaagtt ggtttccatt tgatgatctt ttaccaaacg atccaaactt gtttgaatct
     2641 cgcgagcgag cttttctttt tctcgaaaaa gattataaag tttttcactg gcatcagaac
     2701 ggatttcgcc gctggaagtg agaatttgat caatcgctga aagtggctct tccgccgtca
     2761 tcaattggga gccgattttt tgtgaccagt tattgttgag attggaaagc gcctcttgca
     2821 gagctaaaac ctcgaggcaa aaaccccgga tatccttgat ctcgagagtc ttgagtatgg
     2881 cttttttctt aaggcgggga aaccaggtag gaaaaagatc caagctctgc atatagggtc
     2941 gcacgccttc accgaggacc gcggcggctt cggcaatttc ctgaaagctt tgttgcgcca
     3001 actcgggtgt cgcaagaggg gccatttcca agattttact cttggcggat tcgctggtga
     3061 tgtagccacg aattttttct agaatttcaa tccaatcaag agcgatgagt tcttgcatct
     3121 aatacacctt taacaaataa accaatcaat gccagtaaaa ttaaaatcca atcaaagtga
     3181 ccccacaata caaaattggt ggtgggggcg ttttttttca attgcaggtc ataactgcct
     3241 tgccattctt tatgcaaggg tgaatgttgt aaaacatcac cgttagctaa gaccactgta
     3301 ctaatacccg tgtttgttga gcgaatcagg ggcctgcgaa cctcaatggc tcgagccagc
     3361 gtcattaaca tatgctggcg cggttcaaaa ggccatccaa accaagagtc attagtgaca
     3421 ttggcaagaa tgtcagcctg tttttcagca aggcctcgcg tgaactcggg atagagtcct
     3481 tcatagcaga cctgtgcgcc gaatttgatc tcgccttcag ggtgggggta aatgagaatt
     3541 tctggtcctt gacctcggcc aaaattggaa atgaaaggca aaagttttaa taaaattgga
     3601 aaacgctcgc tgaagggaag atattcacca aaagccagaa ggtgggtttt atgatagggc
     3661 tttcccaatt gatggccatt ggcatccacc aagaaaaggg cattgtaggc aagacgtggc
     3721 gacttttctc caggtgcatc cttggcatag gctcccgtaa gcaaggtttt cctcaggggc
     3781 tgcaaccctg cggccaataa aagaggaatt ctcgcgtggc tattccaacg gtcaaggtaa
     3841 tcagggaaag cggtttctgg ccaaacaatc aactccgtgt taggagcttc ggccacggcc
     3901 tggcgagtga gttgcaagaa cttatgcaaa atctcatctt gaaagccttg gcccttttca
     3961 gcatagactt tttcctcatt gccaatgttc gcttgaatga ttgaaatttt taatttgcga
     4021 tcaaaatcat tccaagcctg tccatggaaa tagccgctca aattaaagaa cgtcaaaaag
     4081 ctaacaaata gaaggctgtg cttttgccaa gctctgcgtt gatggcgatg ctcccacaac
     4141 caggccaacc aagcattgag aagaaggagg atcgaagaaa gcccttcaaa gccgatgacg
     4201 tcagcccaat tgtaggcagg agctttgatc caaaataaag tatagccgag atgccattga
     4261 aaaagactcg gccatattct ttcagcgata cttaagagga gggcagaggc ggcaagagtt
     4321 ttgcttaagc tccaggaata tttttctttg aggaaaacga cgagggctgt ggtcagtgga
     4381 atataaagat gcgccagagc tgcgaagagt aaaagcgtca gtaaggccac aggtaaagga
     4441 aagccgccga actctttagc gacatacgca atccaatgaa agccaataag agttaaggtg
     4501 aattgagtcc accatccggc ggcaaaacct tcttgccagg ttttggctcg gtgacaaaga
     4561 aaaagccaga gaggagcata gccaaaaagc agtgcccacg gtggaagtgg aatatagcta
     4621 gtgccgatca gaaacccgga caggagtggc cataaataat ttcgcggaga atagtgcttg
     4681 aaaaacatct taattttact catcatagac tttgtaacac taaggatgga aatggaaaag
     4741 ctaagactgc gttgccctct atgcgctaaa ctttatgagg tcagcgaggc tgatataaaa
     4801 agcaattcgc cgcagtttga ttgcgtttct tgtgcgactc gttttggttt tcaatatgag
     4861 gggcccactt tgcggcatat tgaaaccttc gtggtggatg tttcacataa taataagccc
     4921 gacaataaga tcgatagtaa atttgagagc aaagtccaat caaaaacttg tcccaagtgt
     4981 ggggccatca acgaaaataa gacgggcaag aagatggatg agtgttactc ctgccacgtg
     5041 attttttctc gtgtggaaga actgccttca gatccaactt tgaaggcgca acccagtttg
     5101 gtgcgtaagt ggaaaaacct cattatgaac ttcaataata aagaacttca tgaggatttt
     5161 ttaaagagtt gctatcaatt ggatgcgctt cgctttgcaa ttttgaaata cgaggaactt
     5221 aaaagtgccc agggtggtgc agatgagatt tcggaacgca tgatttttaa agctcatggt
     5281 cttttaaagg tgacgctttc cagcaaaaca gaaggagaac cgcatttcca aaaggaagcc
     5341 cgaagcgccc gccaaacttg gcataagtat gttttatggg ttcctttggg cgtgtcgatt
     5401 ctgatgatcc tgatcggctt ctttagcctg agtttacgca atctggtagg ggctggagtt
     5461 tcggtcatgt tactgacttt cggacttatt cttttttgga aagggagaat ctctctttcc
     5521 gactttcatt agaaatttat tctgaagttt aggtcattca attcgtcgga gagttttgct
     5581 tttctccaac tcgtcctact ttttccatat tcttaataag ggcttttccg aagctggtca
     5641 tttcagaagt ggtgtctttg ggttttccca cattgggatc cacgctgaag gcttcgttgc
     5701 tggatttgcc catgttcgcg gcggccacgc cactgaggcc caagccctga tccaaaatgg
     5761 ttaaagcttt atagagattc aactgacgag aggttcgtgt cttcgctacg agcgaaacct
     5821 gggcgtctcc ggtggaaaga atatattttt taatatcgac cgctttaaaa gcttctcggc
     5881 gagccatgac cagaacggct gcccccgtga caaacgccgt tgcttgcgaa gttccggtca
     5941 agtagccata agcatttccc ggtaagcatg ataaaatatt ttgtcccggc gccgcgatgt
     6001 ccacggtttc aattccgtaa ttgctggaag aaaggacttc cgtgctggga tcaatagcgg
     6061 tgattgaaac gatattggag agcccataat ccgcgggata atatttgaac tgatcagaat
     6121 ttgatctttc atttccagcc gccgccacaa agagaatccc ttttttctca gcttcgacga
     6181 tggcgtcatg ttcttccttg ctgtattcag tgccgccgcc ggagtagtta ataatattgg
     6241 ctcccatttt gaccgcatat ttaatagcgg ccacggtatt ttttaagttg tcggtatttg
     6301 gaattttcgg gtcatagtat tttaaaatca tcaagctgac ctcgggagag atgccactga
     6361 ttcctttgtt gtttccagct ttggcgccaa caatgcaggc gatatgtgtg ccgtgaccgt
     6421 gattgtcagc gagtgtgcca ttgttggaaa caaagttcca gccatggaca tcatcaataa
     6481 atccattgcc atcatcatca ataccattgg aagctttgtt gcgtcctttg ctatcgagac
     6541 cggcttcgcc tggattagtc cagatattcg agtctaagtc ttcgtgttta atgtcggccc
     6601 ctgtgtcgat gatggcgacg atgaccttat ggctgccttt ggaaacagac caggcgcgag
     6661 ccgcatcgga cttttttaaa ccccaagcct gatggatcgc cggatcattg aagagcacgc
     6721 tgggttcgtt ctcaatgggg tccgttttaa tggtgatttt agactcctca agacgtccgg
     6781 actttttgag cgaggtgctg ctgcgagtgg gagaatcact ttgcagatgc tgataggttc
     6841 cgaagacacc gatgaaaaga acgaaaacac caccgatgaa aacgctgagt ttatgtgacc
     6901 gcatgagagg ccctcccgct ttaagtatcg gtgaaaggag agggaggctt aaacgcggct
     6961 tacactttta ggacttttaa agaggactcc atctcatttt gagacgaatt tgcgggatgg
     7021 cctgtcttta tttggctagc ttcaaccact cttctggaaa aacgatagat tcttccgtct
     7081 gattaaacct tttgtagagc acgaagttgc gcatcaccaa cttgatgtaa gttctcgtct
     7141 catcgtaggg aatttcttcg atgaactcca aggcatcttc acgatagcgg gtcttaagcc
     7201 acccacggat ggcgctgccg ctggcattat aggcggacac agctaaaata tatcgctgat
     7261 cgtactgcat aagtaaattt ttaagtgctt ttgttcccag gggcacattg atttcgggat
     7321 cgtacaaatc ctccgcttcc tggtaaggaa ctttggtgtt gtgactgagt tcttttgcca
     7381 ggctcggcag aagttgcatc aagccaaaag catccaccgg actgcgcgca cgggaattga
     7441 aggcggattc ctgccgaatg atcgaaaatc caagctccgg cggaatttgt tctgtttggg
     7501 ccgcttgaat aatcaaatct ttgtaatcgc gagggaataa aagctcagga tgtttctgca
     7561 ccattttatc ttttacttcc agtggcaaag tattaaaagc agcaaacaag ggaagataga
     7621 gattggctct cgcatagctg gttaaaacaa taagccagct ctgttcatca ttgcggcctt
     7681 gttggcgcag gtcctgggag agttggtcaa tgattttttc agagaaagta ttttctccca
     7741 cagccatcaa ccattctgtt tgcaatgcgg aaagaggtcc tatctcttta aggttaaaca
     7801 gcgaataagg aaactccttt tcagtgcttt ttatcgctga gaagctttgc cctagctcac
     7861 gaacagcgac cataccatag tatccaatgg ggtcctcttt gatgatttgt tgcagaagaa
     7921 tctttgcttc ttcgggttta tttaaatttt taagagcgcg tgcatgccag aaagagctgc
     7981 gcattttgtc tgagttgtct tttgcttttt gtgagaactc ctgaagcttc gccgcggcag
     8041 cttcatattt tttcatttta tagaggaccc aaggatgaag ccataaaacc cgctcgcgaa
     8101 tgttcgaact tgaaagaggc tccttgagac tggcctcata atagtctgag gctttagcaa
     8161 agtcgccttt ttcctcagcg atgcggccca ggataaaata aatttcgtcc aaagggtaaa
     8221 cgccttttaa ttgtctctgg gcttcgttta aatattgaag ggccaggttt aatttatctt
     8281 ccgtccaaag agtgcgtact aacaggatat agctttcatg gagatgctta agagtttggg
     8341 agttcttttt gtcagcttta tattgagctt tggtggaatt caccaactga gtagtggctt
     8401 caatataggc gttcttattt tgcgcgacct tgtacgtcat gcgaatattc ttgagtgctt
     8461 gaaacttatc atcttccgag gcttgggggc tttttaagat cttcttgtaa agtgccaagg
     8521 ctttttcgaa ctcccggtgt tggcggaaat ccatggcggc tgcgggcagg tctttgagcg
     8581 ttaagttggg tttcaagcga gggaaagagt gatagagatg actttgcacc gtttcaattt
     8641 cactggcgga cttcacggct tccgcaatct ttaaagcttc aaccagatat tgttcctttt
     8701 ttttaggatt ggtttcatgg cgagccctct ctttaagagc cacgaggtca tcgtttttat
     8761 cttcggtttt gcgcgctgtc ttaaaaagaa cgtcagctaa gacatcctga ctccatttgt
     8821 agctcgtgcg atagagctca aggttcagag agggcaagcc tgccgtgtca gcgcaggctt
     8881 ggtgggctcg caggagggca atctccttca gtggaaaatc aaggtctttg gccaagatgg
     8941 taaactgaga gcaagccagc tcaggatttt tttccagact caagagtcct tggcggtatt
     9001 tcctccacca gatttggttt tcactattta cttgcagggt ttcgagctct ttcagcgtgt
     9061 gattgcgatt ttgaatgatg aaatcatagc tgggagtctt tacggatgtg gtcgtgcacc
     9121 ccagtagtaa atttgcaaaa accgcagcgg caatgaaatt cattaggcga tacggcctct
     9181 ctctagcgct ctaatacggt cttccaacgc cggatgagtc ataaataaat cggcgatact
     9241 gccctttttc tcgcgtcctg aaattttcag agttgccatg gcgtcatctc gagagggggg
     9301 agcctcgtgc aggctctgca agcgttgcaa agcggcgatc atatttcctg gggaagaaac
     9361 cttggcgctg ccggcatccg cgcggaattc tcttttgcgc gaatagaaat tcaccacaat
     9421 ggaacccagc aaagtgaagg caatgtcacc gataaggaca cagataaagt gaacgatgcc
     9481 tcggtatttt tcatccacat tgctggccaa aacattggcc aacaagcgag agaagaacat
     9541 ggcaaaagca ttcaccaccc cttgaatcag agtcatcgtg accatatcgc cattggaaat
     9601 atgggaaatt tcgtgaccga gaactccttc aatttccttg tcattcattc tttgcagtaa
     9661 tccagtggag accgcgacta aagaattctt tttactcggc ccggtggcga aagcattgac
     9721 gtcataagat tcgtagatgc ccacttccgg catcttggga agctgggccc gtcgggcaag
     9781 ctcatgaacc ttttcaacca attgacgaag atccgggacc tgggtgttag gatcaattat
     9841 ctgcacacgg taaaaatgtt tggctgcaaa ttttgacatg agtaaagaaa taaaggctcc
     9901 acccataccc caaaccaaac agaaggccat caatgacggc aggtattgat tgaatccggc
     9961 taagttcaag aaatggctga tgagggacca ggctataccg atggtcacca tgactaagag
    10021 attggtaaga ataaaataac caaaacgtct gaaaaatttc atatagaaac tccctaggag
    10081 gtgatttcat ctcctaggct taatttggca tctctgcaaa gcttgtcaag tagtgatggt
    10141 ctctttcggc tttttagagc ctgttctatt cggtccgtca ttttgtgtgg tgctaaattg
    10201 gaacttcagg actgtgccct ccacttcgac gctcacccgc cctccgtcga caagtcggcc
    10261 aaagagcaac tcatctacta ggggcttctt taggtgttcg tccactgtac gcgctaaagg
    10321 gcgggctcca tagactttgt catagccttt tttcataagc cactttaaga cttcttgagt
    10381 cacaagcagt tctacctttt tctcggccag agtcattttt aattcgttca cgaactttgc
    10441 gaccacctta agaataatgt cttctgacag gtctttaaag cttaccactg catccaagcg
    10501 attgataaat tctggagaga aggctttttt aatcgcctcc atgctttgca tgctgcgatt
    10561 ttcctccaca attccaatcg agccacgcgc gacttccatc gctccagcat tgctagtcat
    10621 cacaaaaata acatttttaa aatcagcaat gcgaccatgg gaatcggtga ggcgtcctgc
    10681 atccatgact tgtaaaagag cattggtgac gtcgggatga gctttttcga gctcatccaa
    10741 gaggagaacg gcataaggat ttttattgac cgcttctgtg aggaggcccc cttcttcata
    10801 gcctacgtaa cctggagggg ccccgatcag gcgggccact gcatgtttct ccatatactc
    10861 actcatgtca aagcggtgga aatggatgcc cagaatatgc gccaattgtt tacagacttc
    10921 cgtttttccg acccctgtgg ggccagtgaa taaataactg ccaatgggtt tatcagggcg
    10981 ggaaagaccg ctgcgtgcaa atttaatact ggttaccaaa cgatcgatgg cttcgtcttg
    11041 gccatagata agcgctttga gctttttatc cagatcgcgc aactggattt tttcgttgga
    11101 agaaatattg gcaataggta ggcctgttat tttcgcaatc acttcctcaa tagcttgaag
    11161 gtttatagtg attttgcttt gagcattttc tgcactcgtg ttttggcgaa ggcggaagta
    11221 agctccagct tcgtccatca catcgatggc tttgtctggt aacaacttgc cgtgaatatg
    11281 cttaatggat agatccactg cggccttcag ggcttcgtcg gtgtacctga cgttatgaaa
    11341 ttcttcatag gatttgcgga ggccctttaa gatggccacg cagtctgcgg cagtaggttc
    11401 attgacatca atccgttgaa agcgccgatt cagggcccgg tctttttcaa aatactggcg
    11461 atattcagta tgggtcgttg agcctatgca actgatgtcg ccgttggcca gggcgggctt
    11521 tagcaaatta gatgcgtcca aagagccgcc actggtcgcg ccggcgccaa cgatattgtg
    11581 aatttcatca ataaataaaa tagcattggg acgtttctca atttccttga tgacagcttt
    11641 gaggcgtcct tcgaaatctc cgcgaaattt tgtccccgcc agcaaggtgc cgacgtccaa
    11701 agaataaatc accgcggttt tcagtttttc tggaacttcg ccgtggacga ttttggccgc
    11761 caacccttca gcaagggccg ttttgccaac gccgggttcg ccgatcaata gcggattgtt
    11821 ttttgttctg cgagaaagaa tctgcacgca gcgttcaatt acgtcctctc gtcccaccaa
    11881 ggggtcgatc tttccggcct tggctttctc gttcaaattt gtgcagaagc tttccagagg
    11941 agaattttta ctttcgtcgg cgggctggcc atcaatatcc atccgcggag ccggggaggc
    12001 cgagttcttc tccgccgtgc cgctgacatc tttggccacg ccatgggaaa ggtagctaat
    12061 gacatcaaac tgactcaggc cttgttgcga aagggcgaag acagcgtgag aatcttgttc
    12121 atagaaaaga gccaccagta ggctgccttc attgatttga ttgcgtccgg agcttttgac
    12181 ttgaatggcg gcccgctgaa gcaatcgatg gcaggccaag gtgaactccg gagtccagct
    12241 ttcaaagccg ccataagaat ccaactgctc ttgggtgata tgcggagctg tctttttcag
    12301 ataatcttta agatccaatt tgagtttttg tacattggct ccacaagctt gaataatctc
    12361 taccataagg ggagactcgg tgagggcaag caggatgtgt tcgagggtga cgaattcatg
    12421 gtaagccttt ttagcgagtt ccgtagccac tgagagtcgt cgctctaatt cacgtgtcat
    12481 cataaactat tcctccataa attattcttc ttccagagtg cttttcaggg ggtgctcatt
    12541 catttgcgag tactggttca cttgcatgac tttcatttca gccacttcca aggtaaacac
    12601 tccagcggcc ccggcccctt tcttgtggac atcaatcatg atcttggtgg cttcttcttc
    12661 gcttttgccg aaaaagcgcc gcagtatcaa taccacgaat tccatcggag tataatcatc
    12721 attgagcaat atcaccttat acatttttgg tgtgtctatc cgcggaatag tttgaatgga
    12781 agtttcccct tctgtggaaa taaaagggaa gttcttgggg gtgtcgttac tgttaggtcc
    12841 agagctgttt ccatttattt tactcatcgc gaatcacctt ctttgttttc tttttagggc
    12901 ctaaggtcta gtgagttgcc actcccactt aaactcaccc caacaaatct agggttatta
    12961 tagaatgaat gtcttgtttt gagaaggaat cttgattttt aaaatgcaat ttggcacgct
    13021 tgttaaagtc atccatattt atttttttag aacctaatta aagttaatga actaattttg
    13081 gaggtttctc atgaaggacg taggcgtaaa cagaaatcaa gcgggtttct cgcttgtaga
    13141 attgatggtc gtcgtcgcca ttatcggtat tttggcatct ctcgccatcc cgtcagtcgg
    13201 caagtacatg gcaaaagccc gtcagtctga ggcgaaaacg caattggcgt ctttgtacac
    13261 tgcggaaaag gccttctact ccgaatatac tcagtatgat tctcgttttg gcgccattgg
    13321 atattcgccg gaaggtaagc tacgttacaa tgtgggtttt gcggcagcgg tagcgtcaac
    13381 gacttacact ggaacgttag ctggtaacca acttttattt aacactctta catattgtcc
    13441 agcaacgact ggtgccgctg ctcttggtgt aactggatgc gggatacttc cgggagccac
    13501 taatgcgcca ccaccagcta ttaatgccgc cagcacagtg gcaaatccag caggtgcgag
    13561 tacatttatc gccgaagcta ctgcagttgt gcatagccaa ggtcttgctg atcgatggac
    13621 agtaaatgag tcaaaaaata tgctcaatcc tcagaatggc gtaccttaga tcgatgtcga
    13681 agtggttaga ttttacaaaa agctcaagct tcagtttgct cgggcttttt tctttttttc
    13741 taattatgta ctcacatatc tatgcttcta aaggactttt agagaaaccc cgggatttgt
    13801 tgtcaccacc agccggtgtt gaacatttta cctttggtca tcgtgatgtt acagcggact
    13861 tcctgtggat tcgtgctatt caagattttg attattgtga tcagcaaatc gcaaaaaatc
    13921 tttgtgttag taaaggttgg ctttatcgca tgctgaacac aattactgat ctctcaccta
    13981 cgtttcgaat gccttatgcc acaggggcgg tggctctgag tgtcttggtc tctgatattg
    14041 aaggagctgc ccgtatattt gataagggca ttgaaaacta tcccaaggat tggaggatcc
    14101 tttaccgggc agcctaccat tatctctatg aagtcaaaaa caagaagaag gccgctgatt
    14161 tatttattcg cgcaggtcga gagggggctc ccgcttgggt gttttctctc gctgggggac
    14221 tttacaatga ttctaacgag cgagctgcag cagaggctgt tctccaggag atgctcaaga
    14281 cagaaatgga acccatcatc atcaagcgcc tccaagaaaa gctcgaaaaa atccgaactc
    14341 tgtaggtacg ccgtaccttt ttcggatgcg ccatgtcgat ccgtctcaca ggagatatgc
    14401 aaagttaata atggtgaaag taatcttctt cgatgacgtc ttttttgaaa ggtctcccgc
    14461 atagattaaa accggtcgga tagtggaggt cttggggtca agtaaaatcg tacatagtta
    14521 tccaggagac gaaatctcac gatcttaggg ctatctttaa gagtttttag ggcccgtaac
    14581 aagactttgg acaattttcg tgtaagagtc tctgcggcgt tcaaatatat cgtaaatatt
    14641 ttatcgaatt ctgaaaatag aaaacccgtt tttgaaaaac aaagatcccg atagctgtct
    14701 tcggcggagc gcgaatagtc aatttcctct aggtatcggg agacgcctcc ggtgagacag
    14761 agaattctta atttttccaa ggtggaggta tgaactaata cgctgccaca aagaaccaga
    14821 cgcaatcggg aatttttttt aaagtggatg tcccatgcta tcttgagctt tcctggaaaa
    14881 tccggatcgt gatggcccac caagagatct cgtcaagaaa gaccaggaca tgttctttct
    14941 cgatgtgggt gggtagaagt tgaaaggcgt cactccagtc tttaaagacg acttcagggg
    15001 gctgccatag cgcgagtttt tgcttcgtca gcgtctcaac actccgcatc cgaaaaaagt
    15061 acggcgtacc tccgggggga ttcttgactc cttgctaaaa tgcctcatat tcgcagttcc
    15121 gatatttaat attgaatctg tgaatttagg gaataatatg ttgagtcaaa gggattgcta
    15181 tgagattttg ggcgtcgctc gtgacgctga tgcagacact attaaaaaag cctatcgtaa
    15241 attggccatg caatttcatc ccgataagaa ccccggtaat ccggcagctg aagagaagtt
    15301 taaagaagct gccggggctt atgagatatt gagcgatgcg cagaagcggg ctcagtatga
    15361 tcgctttggt catgctgcat tctcaggggg aggccgtggc cagcaggatg tgaatgatat
    15421 ttttgcaaac tttggggata tttttggcga ctttttcggc ggcggaggac agcaaagatc
    15481 ccgtcgttct cgtcatgagc cgcgtcgcgg agccgatctt cgttatctca ccgagatttc
    15541 tttaaaagat gttttaaacg gtttagaaaa agacattgaa ttcgatactg ataaatcctg
    15601 tgacgaatgt catggaactg gagctgaaaa aggctccgct ccggtgactt gtccgacctg
    15661 ccatggttca ggtcaagtgg tgcgctctca aggcttcttt agcgtcgcct cgacgtgtcc
    15721 gacctgtcgt ggagagggtt cgaccatcaa gaatccctgc aaagcctgta agggcaaagg
    15781 tcgcgtttcg gaacatcgta aaatcctcat taacatccct ccgggcgtcg atacggggac
    15841 gcgattgcgt gttggcaatg aaggagaagg tggcttcaat ggcggccctg ccggggatct
    15901 ttatattgaa attcgtgtta aagatcatcc ccgctttgag cgtgaaggag aggatcttca
    15961 tagcaccgtg tccgtgcctt atttgcaact tcttttaggg gcagagatca aagttgagac
    16021 actcagtgaa cctgccatat tggatatccc taagggcaca gcgaatggaa aaaccctgaa
    16081 gctcgcgggc caaggtctgc cttctttgcg gggtcaacgt cgcggggatc tttattgcac
    16141 cttagaagtt gaatttcctg aaaaaatctc aaaagaagag gaaaaaatct tacgggagtt
    16201 ggcaaaatta aagggaattg aagtcaaagg tgaaagcggt gggctttttg ggcgaaaaaa
    16261 atgagttaaa aaacacctag gcaccttgac ggtttataaa gccgacacat gtttaaattg
    16321 ttaattaagc tagatatttt gtaaggagta aacaatggga aagattattg gtattgactt
    16381 aggtacaacc aactcttgcg tggcaatcat ggaaggtggc gaaccgaagg ttttggtaaa
    16441 tgaagagggc gcgcgcacga ctccttcagt ggtggctttt acaaaggatg gggaacgtct
    16501 ggttgggcag atcgcgaaac gtcaggcggt gaccaatcca gagaacacta tttactctgc
    16561 aaaacgtttt atcggccgca gatttgaaga agtgaccgaa gagatcaagc tggtccctta
    16621 tacagtggtg gctaagggca atgattgtgc ctttaaatta tcaaatagca aaactgtttc
    16681 tccagaagag atcggtgcag ctgttttggc taagcttaaa aaagttgctg aagattattt
    16741 gggtgagccg gtgacagaag ccgtgatcac agttccagct tatttcaatg actctcaaag
    16801 acaagcgaca aaagacgccg gtcgtatcgc gggtcttgat gtaaaacgta tcattaacga
    16861 accgaccgca gcggctctgg cttacggttt ggatagaaag aaagatgaaa aaatcgttat
    16921 ctacgacttc ggtggaggaa ctttcgacgt ttctattctg gaagttggcg acggtgttgt
    16981 tgaagttcgt tcaacaaacg gggacactca cctgggtggt gataacttcg ataccgtgat
    17041 tttggaatgg ctcatcgctg agttcaaaaa agatcagggt attgatttga agagcgataa
    17101 aatggctctg caacgcttga aagaagctgc tgagaaggcg aaaattgaac tctcttctgc
    17161 gcaagataca gagatcaact tgccattcat cacggcggat caatcaggac cgaaacatct
    17221 gcaaacaaga ttgtctcggg ctaagtttga tcagatgact gagaatctgg taaaacgctc
    17281 catggagcct tgccgcaagg ctttggaaga cgcgggctta aaatcctctg agatcgatga
    17341 aatcgttcta gtaggtggtt cgacgcgtat tccagcgatt caaaaagcgg tcaaagattt
    17401 cttcggcaaa gagccgaacc gcacagtgaa tccggatgaa gttgtggccg tgggtgctgc
    17461 ggttcaaggg ggcgtgcttg ccggtgaagt gaaagacgtt cttcttttgg atgtgattcc
    17521 attgagtctg ggtattgaga ctttgggtgg tgtcatgacc actttgatcg aaagaaattc
    17581 tgcgattccc acgaagaaga cgcaggtgtt ctcgacggcg gcggataatc aacccgcggt
    17641 ggatatccac gtccttcaag gggaacgtaa aatgtccaca gacaataaga ccctgggacg
    17701 ttttgaatta accggaattc caccagctcc tcgtggtgtt cctcaggttg aagtgacctt
    17761 tgatattgat gcgaacggta tcttgagcgt atctgcgaaa gacctgtctt ccggtaaaca
    17821 tcatcagatc aaaatcacag cccaatctgg tttgactgag gaagaagtta aaaaagcggt
    17881 tcgtgaagct gaattgcaag cggatgccga taaacaaaaa gcagaagcgg ttcaagctca
    17941 caacaacttg gataacttga tctatcaaac agagaaagtg atcaaagatt ctggagcgca
    18001 aattccagag tctgaagtga aatccgcgaa tgagactgtg gctgaagcaa aaaaagttct
    18061 cgagaataaa tcggcgacag tgacagaact caaagcggct tttgataaat tgcaagctgt
    18121 gactcacaaa ctttcttctg aattgtacaa aaaacaaggc ggagaagctg gtggcggagc
    18181 tagcggagac ggtgcttccg gtgccaaaga atccgaagga gccgcaaaag gcaacgacga
    18241 cgttattgat gcggattaca aagacgtcaa ctaaagcctt aactgaatat ccttgatttc
    18301 taattgtaaa ggctgggaga atttgattct tccagccttt tttgttttca aaaatagtgg
    18361 aatgagagga tgaataggtg gcgctatgaa tttcgatcaa tataaaaaaa tgtatgaaga
    18421 ttcgatccgc aatcctgaag aattctgggg taaacaagcc gacagacttc attggttcaa
    18481 aaagtggaat aaggtcaaag acacatcctt cacaagcccg gtttcaatca aatggtttga
    18541 gggaggagag ctgaatgtct catacaactg tattgatcgc catttagctg ataatagggc
    18601 cgataaggtc gctcttattt gggaagcgga tgatccccat atccccgcaa aaaaagtaac
    18661 ctacaaagaa cttcatcaaa atgtgtgcaa gtttgccaat attcttaagt gcaacggcgt
    18721 aaagcgcgga gatcgtgtca ccatctatat gcccatgatt ctggaaacct cctatgccat
    18781 gcttgcgtgc agtcgtattg gagctattca ttcagtgatt tttggaggct tcgcgccgga
    18841 ttccattgct gacaggatat tggattgcga atcagatttt gtgattaccg ccgatgaggg
    18901 ctttcgcggc agtaaaatca ttcccctgaa aaagaatgtc gatcaagcct taaagcgaac
    18961 gccaaaggtg cgcaaagttt tagttattaa acactctggc aatgccgtga attttgagaa
    19021 agaccgtgac ctttggtatc acgaagaaat ccttcaggtc gatgcgcaat gtgaacctga
    19081 aagaatgaat gccgaagatc ctcttttttt attgtatacc tcaggttcca ccggaaaacc
    19141 gaaggctgta ctgcatacca cgggcggcta tctgctgtat gcgagcctga cccataagta
    19201 tgtttttgat ctcagagaaa atgatatttt ctggtgcacg gccgatgtgg gatgggtgac
    19261 tggacatagt tatttaattt atggcccctt agccaatgcc gccaccactt tgatgtttga
    19321 aggcgtgccg aattatccaa atttctctcg cttttggcag gtggtcgaca agcatcaggt
    19381 cactattttt tataccgcac ccacggcgat tcgcgccctc atgcgagagg gtgatgaaca
    19441 cgttaaaaaa tgttcacgac aatctttgcg tcttctcggc agcgtgggag agcctattaa
    19501 cccagaggct tggcattggt atcacaacgt cgtcggcgac cagcgttgcg ccatcgtgga
    19561 tacgtggtgg caaactgaaa cagggggcat cctcatttct cctttacccg gggccatgga
    19621 gttgaaacct ggctcagcca ctttaccttt tttcggaatt aagcctgttg tgctcactcc
    19681 cgaaggacaa cgtcttgagg gagtttgtga aggcgtttta gccctcgagg attcttggcc
    19741 aggtcaaatg cgcacggttt ataaagatca ccagcgtttt gaacacacgt atttcgcaag
    19801 ttacaaaggc tattatttta ccggagatgg ttgccgtcgc gatgaagacg gttattattg
    19861 gatcacgggc agagtcgatg atgttttgaa tgtctcgggc catcgcattg gcacagcgga
    19921 aatcgaaagt gctttggtgg gccatgcggg agtttcagaa gccgccgttg tgggctatcc
    19981 tcacgatatc aaagggactg gcgtttatgc tttcgtcact ctcagaaccg gccttgaagg
    20041 cagcgaagaa atcaaaaaag aattaggcca atgggtgcga aaagaaatcg gcgccattgc
    20101 cgcgcccgat ttaatccaat gggcttcttc aggtcttccg aaaacccgct ctggaaaaat
    20161 cattcgccga attttaagaa agatcgccga aaatcatccg gatcaattgg gcgatatctc
    20221 aaccttggca gatcccagcg tggttgaaga tctcgtaaaa aaccgactgc aaaagtaatg
    20281 aggaaattca ataagcaatc gtgagcacgg tggtacgttc ggaatgcata ttcaccaact
    20341 ttagagtgcc agtgccctta acggaaagca gctggtcctt cttgatctca aaggtttcat
    20401 cattggttct tgcaatgaag cttccctgta agacataaat ttcgtaacgg aaattcttct
    20461 caactttcat ttttcgaatg gattttgaag cgatttctat agatcgaatt ttggctgtat
    20521 tctcaaaggc ttttacataa tttttgatcg ctaagttatc gccaatatta atacaagggg
    20581 tgttgagttt tctctccacg tattcagtgc gcaatttcat ttctatgtcc attttcgaag
    20641 ttctgctgaa gaggtcctct aggggtactt caaagtaagt agaaagaata aaaaggcttt
    20701 ccaatgtgat ttgtccctgg cctcgttccg ctctgcttat ttggcattta gaaaggctga
    20761 tcttttcact caactgttct tgagttagcc cttctcgctg gcgcaggtta atgagcttcc
    20821 aagcaatatt tcgataaatc tcagtcatag attctttgcc aacgtacact tctttattag
    20881 gatcaaattt gcatttgtag gacccttaaa ctcgagtggc attatacgcc agtgagttgc
    20941 atataatgcc actcgagttt aaggggtccc tttgaactta aagatccagc aaagtttgga
    21001 ccaggctctc gttgctcagg tgtataaaaa agaagttcaa cgagggttgg gagtcggaac
    21061 tccttgtctg gagggcggat cgagagtatt cagaggggat atgcgaaatg ctcccgggct
    21121 gagcaagccg attctcaatt cacaggttaa ttttccgctt tgtttggcgc cgatggtggg
    21181 attgagccat gtggctctgc gcctgactgt gcgtgagtat atacctcaag gggcgaagac
    21241 catttggccc accgagatgt tgaactcgcg tcgtctgcca caggagaatt ttgccacgaa
    21301 cccgcatacc ctgcggggtc ctcaggaaac tgaattggtg ccgcagattt tgggaaatga
    21361 agagaaggcg atcagcgaga gcgtcaaaaa attgatttat gagtggggcg cggaggggat
    21421 tgatatcaat atgggctgtc ccgtgcaaaa agcgcttaga cataattatg gagtggcgct
    21481 catgggggat cctgaatatg ccgcgcgtat tgttgaaatg accgtcaaaa attccacggt
    21541 gccggtgtcg gtgaaattgc gggcagctcc tcagcctgga aaagattctg aaagtcttaa
    21601 tacctctgga aattactctg gaaatgattt tggttatttg cataactttg tgcagaggct
    21661 taaagaggcc ggggctagct gggtttgtct tcatccgcga accgccgcgc aaaaacgtcg
    21721 gggcgtggcc gattggtccc agatcacgaa gcttcgagaa gctgtggatc ttccgctgat
    21781 cggcaatggc gatgtgcaaa cggcggagga tgcattgcat atgctccagg aaaccggttg
    21841 tgatatggcg atggctggcc gcgccttggc ggctcgtcct tggatgatgt ggcaattcgg
    21901 cgagatgttg ggatttgctc cgccagtggg acgcgaagga caacgggctc cgcaaggccc
    21961 tcttgaagaa ggcgccgagt atgggaaagc tttattagcg ttgattgagc actgtgctca
    22021 gtattttacc gaagatatgg ctctgcgtaa gattcgtttt catgtaagaa ccacggaagt
    22081 gtggttggat ttcggccaat ctctggtggg agtctgcgat aaagccaaca acctgaaaga
    22141 tcttcgaatt tcagtgaaga atttctttca agcgcccatt gagatgtgcg ctcgcacgga
    22201 gttgcgccag taaaagccaa taaaaaagca actaggcctg taaaagcgtt tccaactttc
    22261 cttcctcatg aagagctttc atatcgttgt agccaccgat gagaacgccg ttcaccataa
    22321 tgatggggac ggttctccaa cctgtttcgc ttttgatgcg atcaatttct tccggtttgt
    22381 ccgtgaggtc cacttcttcg tattgaattc catattcttc aaaaaaacga atggcacgca
    22441 cgcaataagg gcaggggtct ttggtgtaaa tcacgacttt ggctttttga tttttcatat
    22501 ttttacctca agtggttttt tattataaga tcaagaacag agaatttcca attgcgaacg
    22561 aaaatccaag ggctttttga gcaccttaag ctggtcaaag atggctttag cttcggaatt
    22621 ttttaaggag agagcgcgaa gccattgttt ggtgcggctg accgcgaaat actcattgat
    22681 ataaaaagtg ctggcttcaa agaagcccgg cagcaggtgc ttggtatttg gccaaggatc
    22741 tgtgacgctc ttgttggtgc cgcggatttg tttgaaaata taaggattgc tgagggctcc
    22801 gcggcctatc ataaagtcat tgcagccagt gatctctcgg cagcgcagga aatcactgac
    22861 gttccagatt tcgccgttgg caatgagggg tatctttgtg gtctcgcgta tacggggaat
    22921 ccactcccaa taagccgggg gcttataact gtctgttttg gtgcggcagt gaacggtgag
    22981 ccaagtcgct ccggcctctt cgatggcgcg ggcattttcc atacaaaccg aaggatcttc
    23041 gaagcctaag cgaatttttg cggtgacagg aatcgcggat ggaacggcgt cgcgcactgt
    23101 tttaacgatg gtgtacagac gatcgcagga tttaagcaaa ctggctccgc catcatgacg
    23161 gttcacggtt ttggccggac atccgaaatt gagatcaatg cccaaggctc ccagtttcac
    23221 ggcacggaga gcgttcaggg ccaagggttc tgcctgaccg ccgagaagtt gcacgaaaac
    23281 cggagtgccc tggcgcgtgc gggatccggt tttgagttca ggacaattgc gatagatcac
    23341 ggcttcgggg tggagtttgt cggtgacgcg taaaaattca gtcacaaatt ggtcaatacc
    23401 gccaatttgt gaaagagtgt cacgcatcac ccaatcaacc actccctcca tcggagctag
    23461 gagcaagcgc atctaatgtt ctcctagttt cttaacgatg ttaaagtgct cttccttcac
    23521 gggctgcacc gacaaacgac tgcctttttg aagcaccagc atctctgcca gggagttcac
    23581 ttggcgaagc tcggccaaag aaatcagctg tttaaatttt ttaacatact ccacttgaac
    23641 acagaaccat tgaggttttt ctttggtggc tttttcatca tagtattctg attttttgtt
    23701 gaattgggtt ttatcgggtt gagccaggct cgaaaccctg gcgattcctg cgattcctga
    23761 gggctccgca ttcgagtgat agaagagaac cagatctcca attttcatct ccttcatcat
    23821 aaaattgcgg gcctgatagt tgcgcacgcc ttcccaccag gtggttttat ctctttcaag
    23881 gtgatcaagg gagtaaacat caggttcaga tttcatcagc cagtagttca tagggtccct
    23941 ctcagaaatt ctcttggata agatattgtg cgttatattg attcgtaagg gtgatttcaa
    24001 ggagaaaaaa ttgtaagttc tctcctctaa ggaaaaactt aagatgaata caaaatgggc
    24061 aatcattaca ggggccacag cgggtattgg ttgggcgacg gccgaagagt ttgcagccgc
    24121 gggttattcc ctgattctga tcggacgccg tggaaatcgt ctcaaagagc tgaaagacca
    24181 tctcctgaaa aacttcgttc aatctgggaa gtctcaagag tttaaaactt ttccgttaga
    24241 tgtaactaaa aaatcccaaa ttgatgattt tgtgaaagcc gaaggcctct ccttagagaa
    24301 agtccaggtc ctggtgaata atgcgggcct cgctaaaggc accgagaaag ttcaagaggc
    24361 gcggatttct gactgggacc agatgattga caccaatgtt aaaggccttc tttatctgac
    24421 ccgagccgtt ttaccttcga tggtgaaaag aaagtcggga aatatcgtaa atcttggctc
    24481 cgtggcgggg cgcttggttt atccgggcgg agccgtttat tgtgcgacta aatttgccgt
    24541 gcgcgcgatt tccgagggac tccgtatgga tctagccgga agcaatattc gggtgactaa
    24601 tatcgagccc ggtatggtgc aaagtgaatt ctctttggtc agattcgata acccagaaaa
    24661 agcggataaa gtttatgaag gaatgactcc cttaagtcct aaagacattg cggagaccat
    24721 cctgtggtgc gtgcaacggc cgtcccatgt gaatattcaa gaattagtga tttacccaac
    24781 cgatcaagcc agtgttggcc aagtagtgag gagagcatga tggatttcag aatagaaaaa
    24841 gacaccatgg gtgaagtgaa agttcccgct gataaatttt ggggagctca aacgcaacgt
    24901 tctcgggaaa attttaaaat cggtggcgac acctttcctc gtgaaatgat caaggcttta
    24961 ggtgttctta aaaagtgcgc ggccttgacc aatgctaaat tgggactctt ggatgcaaaa
    25021 aaatgcgaat tcatcgtgaa agccgcggat gaagtgatcg cgggaaatct cgatgcgcat
    25081 tttcctctgg tggtttggca gaccggatct ggtacgcaaa ccaatatgaa cgccaatgaa
    25141 gtcatcgcca accgtgccat gaatatgcaa gggctgcagc ttccgaataa agaaattcat
    25201 cccaacgatg atgtgaataa ggggcagtct tcgaatgata cttttcccac agcgatgcat
    25261 atagcggtgg cgcatcaagt gcaccatcgt ttgttgccga tgcttgaaaa gttgcatggc
    25321 gctctggtca aaaaacaaga agagttcaaa gatgtggtaa aaattggccg tactcatttg
    25381 atggatgcga cgcctttgac tttgggtcaa gaattctcgg gctacgcgac tcagttaaaa
    25441 tattccattc aaagagtgaa aaacactctg ccgcatcttc atcaattggc tttggggggg
    25501 accgctgtcg gaacgggttt gaatacgcat attcaatttc caggggaagc agcgaggacg
    25561 atcgccgcag aaaccaaaat cccttttgtc tccgccgaaa ataaattcga agccttggct
    25621 gcccatgatg ccttggttga agtcagcggc gctttgaata gcatcgcggt ctccttgatg
    25681 aaggttgcca atgacattcg cctcttagga tcaggtcctc gttgtggcat cggtgagctc
    25741 cagcttccag agaacgagcc gggatcatct atcatgccag gaaaagtgaa tccgactcaa
    25801 tccgaagccc tgaccatggt ctgtgctcaa gtgatgggaa acaatgtggc ggtgagtgtg
    25861 gggggggcca atggacactt tgagctcaat gttttcaagc ctttgatcgt ttttaatgtt
    25921 ctgaattcta ttcgtcttct tgcggatggc tgtgagtcct ttaccgatca ttgcgtgagc
    25981 ggtatcgaag ccaatcgcca acaaattaaa aaacatttgg acaactcttt gatgttggtg
    26041 acggctttga atccccatat tggttatgat aatgccgcta agatcgcgaa aacagctcat
    26101 aaaaatggca cgaccttgaa agaggaagca gtcaagttag gacttctttc tgctgagcaa
    26161 tttgataaga tagtaaggcc ggaggacatg gttcacccct tgggcaaata gataaaagga
    26221 gagacgttgg caaacgtaaa cgcaaacgcg agcgtaaaac tcaatccgct cttacaaaaa
    26281 gcgagttgga tggaatcggg cgatataaag ccttatgtct ttgttcgtcc agagaatcag
    26341 attgcgaatc caggaactcg ccataagttg gattggttta gccacgggtc tgtttataag
    26401 gatcctttag atatcgttga agtcaacttt gcggatcgca tctactccat tgaagagcgc
    26461 gctttcggcc cttcgaacat ggccatgccc cgctgggtct tctacgactg cgcggtgatc
    26521 ccaggttttg tcgccggttt tgccgcaaga ccttcagctc ttaagaaaga agtgcgggat
    26581 attttagacc cggtgaaatt tcccgccacg aactcaccga tttttaaaag cagtctggct
    26641 gaggctcaag ccatgattgt gaagtctttg gatgacatgg attgggtgcc gctttcgctt
    26701 tttattatta ttccgacaat gcatcgagga gagtgggtgg cccataatct ttgctcggtg
    26761 aactctttgc tagcgaaaga acatcagttc tatggtctgg gttttctttc gaaggctttt
    26821 ggtctgtggt atgcgaatgt ggagcaatgt tcaggcatga ctcaatgggg aagccccgct
    26881 ttgaagttgc acagtcactt cggtcatctc gaaattgtgg gcgcttatgc gccggtgcat
    26941 tcgcatgcga aaaccgtcac ttatcgcacc caagtgaata ctgtttgctg ggagcagttc
    27001 ttttcaaggg aacaggattt agctttcttg gaaaactatg gtcctagcga ttcgaaaatt
    27061 gaccctaaga acgagcagag catgttggat ctgcaaaaaa agattgagaa agaagagggt
    27121 cctttttatc tcaacgctat ggaaattgcc gctaaaaatt tagatgaatc tttaaccatc
    27181 tacaaaccaa agggatatta agatgccagt gaatcaattg cctcctgtcg cgaaatcatt
    27241 ttttactttt tgcaaaaaat gtaattccga tcgttaccat acagtcctgg ctcacacgac
    27301 ttcgcgaacg gctaaaataa aatgcgaagt ttgcggcagc cagaagtctt actcattacc
    27361 aaaagcgagc agctcttcga cgggaaaacc caaagtcacg cgtccccgtg ctggctctga
    27421 gacagctcgc agatcttctc acaacgcaga gtacgaactg cttttagaga cttacgaaaa
    27481 atccagccct gtcacttaca gtatcaaagg gaaatttacg aagaatcaaa aaattgagca
    27541 tcctaagttc ggcctgggtg ttatccgtga tgcacagttg gagagaattg aagtggtttt
    27601 cgccgacgaa gtccgtaatc tcatccataa ccgtcaataa tgaatcgctg gacgaagggc
    27661 ctcaatcctg agcagatcaa agctgtggaa cacgattttg gcccgctcct tatcctggcc
    27721 ggagccggat ctggaaagac cacggttctg gtttccagaa ccggtcgact tatcgcggac
    27781 cgcattgcca acgcggacga aatctgtgtt ctgacattta ccaataaagc cgccaaagaa
    27841 ctcaaacatc gagtcacgag caaggtcgga tcccaggcga aaaaaggtct gtgggccgga
    27901 acttttcact cctttggttt gcaggtgctt cgccgttttc ataagcaagt gggcctcagc
    27961 ccctactttg gtattgtgga tcagagtgat tgcaattcca tcatcaaaga actgatgaag
    28021 gacctcaaga actccggcaa agacaaattt gatgtcgata aaattctcaa tatgatcaat
    28081 gaacgccgca ccgggatggc tccaaagacg gaggcctttg atgaatacca cgagatggtg
    28141 gaggtcttga ctcccaagtt taataaaaaa ttggaactgc tgggggtcgt ggactttgag
    28201 ggcttattga ttaagccctt ggagcttttc aggaaccatc ccgaaactct ggagaaaatc
    28261 caaaactcct tcacgcaagt gatggtggat gagttccaag acaccaatcg cctgcaaatg
    28321 gaactgattc atcaaatcgt caagacccat aataatatca cggcggtggg tgatgatgac
    28381 cagtctattt atggctggcg tggggctgat gtgcaaaata ttctcaattt tcctaaggaa
    28441 tttaaaagtt gcgaagtgat taagcttgag cgtaattacc gctcgagcac ggagattttg
    28501 gcggtggcca atgcggcgat tgcccagaat aaaaatcgtc atggcaaagt cctgcgctct
    28561 gacatggccg ccgaaacggg cgatctacct gaggttttcg tgctggaacg cgaagaagat
    28621 gaatgtgact tcgtcgtcag tgaagttcag catttcatgc gtcaagggta tggttataaa
    28681 gattttgccg tgctttatcg ttccaacact cagggaggct tgatcgagag ttccctccgc
    28741 cgcgcgaata ttccttacac gatcacgggt ggaacttcga tcttcgaccg caaagaagtc
    28801 aaagacgtca tgtgctattt aaagcaggct ttggcgccga atgaggtctc tctccgtcgt
    28861 atcattaatg tgcctccgcg aggaattggc gagaccacga ttgaaaaact gacggagtat
    28921 tcgaaagaac ataaaatcac ttttctcgag gcctgccagc agtggaaaga ggcggggatt
    28981 ccagaaaaaa ccggggagtc catcgatcat ttattggcct tctgccacac actgccctcg
    29041 cagattttag attataaagt gggctccagt cccggtgctc actttgttga aatcatgcaa
    29101 gaactgggct atcgccagtt tatttataac ggagccgggg atcccacggt ggccgagaaa
    29161 aaatggcaag tcgtggaaat cgtcgggcgc atcctggatg cctatcttgg gcggcgcagt
    29221 tatgaaattg agaatattaa atcatttatt gatgccatga tgcttcgtga tgacgtcaat
    29281 gaagaagatg cacagcaaaa caaaatgcaa ctgatgaccc tgcacgcgag caagggcttg
    29341 gagtttccag tagtgatttt agcgggtctt gaagaggatc ttcttccaca caaaaccttg
    29401 ggttccgata tcgatgagga gcgccgcttg ttttatgtgg gtgtcactcg agcgaaaaaa
    29461 cgcctggtca tgtcccgctg ccagcaacga aaacgtcacg gggtggttcg tccggtggcc
    29521 ccctcgcgct tcctgttgga ggtcgcacaa aagctttata aagaatatcc tgccggagcg
    29581 agacctgtct caggacaaga acgcgaggat cttgtttcga gctttttaaa gggcctgggg
    29641 aataaaacac aaagcaagta aacgcagcct tgacttaagc ttgacttaga aagttcttca
    29701 ggatgggatt aatctcaaat taagagactg atctaggagg acctccgtgg ccagagccaa
    29761 aaaagccttt ccttataaag tgatctcagt gcaaatgctt aaaggcggag ttgctaagac
    29821 aacttcaaca ttgaacatcg gccttggtgc cgccagctat aatgcccggg tcctgtttat
    29881 cgatatggac caacaggcca atctgacctt cgctctcgga gtaggggtgg aagaggctga
    29941 ggaaaagcct gtgtggttgg atattgtgga gaaaaaagcc accatccaag attgtattat
    30001 aaaattgacc gacaaaattg atttgattcc ttcaaacctt aataactccg ttttagaccg
    30061 tgttcttttg aattccaatc gcaactgggc tcaggcggtc aaaagtcctt tggagcaaat
    30121 ttctaaggac tacgacatta tcctgattga tacggcccct gccctgagcg ccaccaatac
    30181 ggccgtggtg gtggcttcag atttggtcat tttaccggtg aatccagatc gtttctctta
    30241 tatgggattg gagaaacacc tggaagaact tcgggaaatc aagaaggact ttgatcttga
    30301 tttttcaaag aagattcttt ttactaagtt tgatgcgcgt gaaaaagtca gccatgaact
    30361 tttacaaaaa tgcatggatt cctttgagca tttgctgatg aagagttata taaggacttc
    30421 cactgaggtg aaaaacacca tcggtacggg aaagacgatt taccatacca aatcaaacgc
    30481 tcgcgaggat tatgatttga tcacccgcga agttttagcg atctcttaag gaagggcgaa
    30541 tatgccaacc agtaaagaat atatatcaaa aaagaaaaaa gcccgcagac gtccaggacg
    30601 tgaagaagtc agtgaagaaa ttagtgaaga agatttatca gagcctgtgg cctctacgtc
    30661 gacttctcaa tccgcttctg agtccgcctc tgagtccttt tcggattcag aagaaactcc
    30721 tctgcgaaaa aagattcaca tcgagttccc gtacagtgaa gctgttcgtg ccaaagttcc
    30781 gaaagctttc gatattgcgg agaatgtggc tgaggaatgg gtgaatgatg gaaatttcga
    30841 aaacgttccc ttgggccatc cgttggcgca aattacggtg gccgcaggac ttcgtaaagc
    30901 caaagaagtg gaaaagaagc tcgaagaaaa aggcgtcttt atgatggcca aaatgggtct
    30961 tgagtatgca aagtcaaaac tccataagaa ataatagggg tcccatcgaa gcgctcctcc
    31021 gtgaaagaat cgagacggct ttcactcccg agtactatga gctcgagaat gaaagccacc
    31081 ggcattcggt gcccgcaaat tctgaaactc attttcgttt gctggtggtt tccaagagtt
    31141 tcgaggggaa atcccgcctg gatcgcagcc gtctggtcaa cgagttgatg aagcctttga
    31201 tggctcaagg acttcatgct ctttcgcaaa gaaccttgac cccgccagag tggctgcaaa
    31261 ttaaagatac tttcgttatg gtgtcccctg aatgtcgggg cggatctcgt cagagctaaa
    31321 gaaaacagaa aaggcttaag cctttttttc ctcttctttg tttttattat cttctatggt
    31381 tagagcgtcg tcgctaaatt ctttgagttg atctttgaga gtgactaaga tggttttgat
    31441 atcgcggagt tgtttctctt caggcgagac tttattcgcc actttcccgc tgagatcagt
    31501 ccaggaatcg agtgcccctt ttaaatctgt atttatatga tcccaagaag gtttggtttc
    31561 atctttgttc ggtttatcca tctattcaca cctacttatt aatttgtaag ggaaatgaac
    31621 tccatttgac aagataattc agactgatag attggatctc ttgtcattct taaagtatta
    31681 cttaaagggg cccatgcatg tctcaagata tcatctatac gatgaagggt gtttcaaaag
    31741 tttatccacc caatcgtttt gtattaaaaa atatttacct ttcttatttc tacggagcta
    31801 agattggcgt tttaggtttg aatggttccg gaaaatcctc tctgctaaaa attatggcag
    31861 gcttagacaa ggacttcctt ggtgaggctt tcccagcgaa acaaatgaaa gtgggctatc
    31921 ttgaacaaga gccacacctg gacccaagcc tgacggtgaa agaaaatatt ttctcgggaa
    31981 tgggcgaatt gcccattctg atgaagcaat tcaatgagat cagtgaacag ttcaatgatc
    32041 ctgaccttga tcccgataaa atggataaac tcattgaaaa acaaggagcc attcaagaaa
    32101 aactggaagc cctgggtggt tgggaggtgg atcaaaagat cgaaatcgct atggatgccc
    32161 ttcgttgtcc agagggagat cttcccattg gcccactttc tggtggtgaa aaacgtcgcg
    32221 tggctctttg ccgtctgctg atgagtgatc cggacatttt gttattggat gagccgacga
    32281 atcacttgga tgcggaatcc gtggcttggt tggagcaata tcttgcgaaa ttccctggca
    32341 ctgtcattgc ggtcacccat gatcgttact ttttggacaa tgtggccgga tggattcttg
    32401 aactggaccg tggcgagggc atcccttgga agggcaatta cacctcttgg cttgagcaaa
    32461 aagacaatcg tatggctcag gagcaaaagg acaatgcgcg cagaaataag acgctggaaa
    32521 gagaactcca gtggattcgt caaggagcca aggctcgaca gtccaaggcc aagtctagga
    32581 tctcggccta tgaaaatctc ttgaaggagc caacaccaga gaagctccaa gaaatgtcta
    32641 tttatattcc gcctggacct cgcttggggg atatcgtggt ggaagcgaaa aatatcacga
    32701 aagcgtataa tggaagattg ctctttgatg atgtcagttt caccatccct cgaggggctg
    32761 ttgtgggcgt cattgggccc aacggtgtgg gtaagaccac tctgttccgt atgatcacgg
    32821 gtcaagagaa ggctgacagt ggcagcttta aagtcggaga aaccgtgaaa atggcctacg
    32881 tggatcaaag tcgcgagacc ttgaacgctg acaaaacggt ttgggaagaa ctttccgggg
    32941 gcaatgagct gattcagctc gggcaaaagg agatgtcttc cagagcctat gtttcctggt
    33001 ttaatttttc tggatcggat cagcagaaga aggtgggagt gctttcgggc ggagaaagaa
    33061 atcgcgtgaa catggcgaag atcttgaagc aaggcgccaa tttactttta ctggatgagc
    33121 cgaccaatga tttagatgtg aataccatgc gcgccctcga ggaagctatt ttggaattcg
    33181 gtggttcggc gattgttatt tcccatgatc gctggttttt agatcgtatt tgcactcata
    33241 ttatggcctt tgaaggcgac tccaaagtgg agtttttcaa tggaaacttc tctgagtacg
    33301 aagaggatcg gaaaaaacgt ctcggagctg atgccgcacc gaaacggatt cgttttagat
    33361 ctatcaatta gcaaaagagc ttattgagca tttcataatt atttttaagc tcaataaagg
    33421 cgctagtagc gcctttattc atatcagggt gaagtttcag ggcgagcgct ctaaaagctt
    33481 tcttcagctc tttttgtgga aaagcaggac taagggaggc cccataaaag agaaaaaagg
    33541 aaactgccac ctgttgctcc tcactcaagg tatgaggggg acgagggggc gacggataga
    33601 tcttatgaac ccgcaactga ggtggggggt ggaagaaggt ctgacggata tggcccatca
    33661 gataagccag atgagcgggt tcgaagtctt gctgagggga cttattcaga tcgcgcggat
    33721 cgcttgctat tttaatttta ctttcaagaa tttgctgaaa gcttgcctgt agacccatgg
    33781 agatcttttc ggttctcaag cggaaatctc gagtctagat ctcttttaga aagtcatttg
    33841 tgacgattcc cacaatagaa agtcatagtt ttgggcagat ttgcctctaa taaagacctt
    33901 tttccctata gaaatggaga ttttttccgg gaaaacctca aaaacttctt aaagaaagat
    33961 ccgtttctcg aggagaaaat gctgttttaa tatgaattct cgagggaaaa ttgagttttc
    34021 tcctctgaaa aattcaattt gagaggggaa aatagaaaaa tcttaagcag tctgcatttt
    34081 tttttggaca aacaccaaga gttggttaca ctgaagtcat tcgagttaaa aaataaagca
    34141 aaccattaat ggaggaatac aaaaatggca aaagcaaaga aagcgactaa aaaagccgct
    34201 cctaagaaaa ctgctactaa aaaagcaact actaaaaaag cggctcctaa ggctgcaaaa
    34261 gctgcgaagg ctcctaaagc taaaaaggct gcctctaaaa gaaagccaaa tccagcgttc
    34321 atgagacctc ttactcctag cactacttta gctgctgttg ttggagcgaa tccgcttcca
    34381 cgcactgaag ttgttaaaaa gctttgggct tatatcaaaa agaacaacct tcaagattct
    34441 aaaaacagaa gaaacatcaa tgctgatgac aaattgaaac caatcttcgg caaaaacact
    34501 gtttctatgt ttgaaatgac taaacttgtt tctaaacatt tgaaatagta tttagatcct
    34561 tttcggcact ctaaatgtga aaagcctagg ctcaaaccct aggctttttc tatttgtcat
    34621 gctataagtt ccccatggga attaagccgc tgtctcttga agaaataaaa aaaatgaagt
    34681 tggcctgccg tatagccgca gacaccctca cttatctcga taaatatatt aaaataggta
    34741 tgaccaccaa tgaagtggat gagcttacca acgactatat gctcacacgt ggggcaaaat
    34801 cggcatgttt aggttatggc ggttatccga aatactcttg cgtgtccgtg aatgaagtca
    34861 tttgccacgg agtccctgac gagacgcccc tcaaagaggg tgacatcgtg aatgtggatg
    34921 tgaccgcttt tattgatggc ttttacgggg acacttcaaa gacttatatg atgggaaagg
    34981 tttcagatac agcccgagac ctggtggaag cggcacgcat ggcccgtgat attggcatcg
    35041 aagccattcg tcctaaggga tatacggggg atattggctt tgagactcat aagttcatta
    35101 cccgtaaagg ttatacggct attagggaaa tcggtggaca cggtattggc cgtgtcttcc
    35161 actctgagcc cttcgttccg gcttatggta aaaaaggcaa aggggagctt ctggtccctt
    35221 tccattgcat caccgtagag cccatgatta accaagggac gaatgaattt atagaatttg
    35281 atatccacgg ctcatcaatt aagtattata aaaccgcaga caacttgctg tctgctcagt
    35341 ttgagcatac ggtattggtg accgacaccg gatacgaaat attaacatta cagagctaat
    35401 tattaattaa attacaaaac atatacaaat caattaacga gctaacaaac aaaggacggg
    35461 aaatgtctct cacgacgaat actgggaaag cagccatgaa gaaaaatatg aaaattgcag
    35521 cgaaagcggt aaccaaaaat acaactccaa caaaaactcc cgttgtggat tacatggttt
    35581 gtaaagaagc catggaaaat ccaaaggtct tcgcggagtt ggcgcgttgg ggacgtgaag
    35641 agatttctat tgctgaaacc gaaatgccag gtttgatggc tcttcgtaag gaatataaaa
    35701 agagacagcc tttaaaaggc gccaatattt ccggctgctt acacatgact attcaaacag
    35761 cggttttgat cgagactctc gtggagttgg gggcaaaaat ccgttggtct tcttgtaata
    35821 tcttttccac tcaagatcat gcggccgtag ccatcgcagc ggcgggaatt cctgttttcg
    35881 cttggaaagg tgaaacagaa gaagagttca actggtgtat cgagcaaacc atcacaggtt
    35941 ggggcaaaga cggcttcaat atgattcttg atgacggcgg cgatcttaca aatatgatgc
    36001 acgagccacg tttcgctaaa gaaatgaaga aaatcatcgg tatctctgaa gagaccacca
    36061 ccggtgttca caatcttgag ctcatgctta aacaaggccg cttgaaagtg ccagcgatca
    36121 atataaacga ctctgtgact aaatctaaat ttgataactt gtatggctgc cgtgagtccc
    36181 ttgccgacgg tatcaaacgt gcgaccgata caatggtggc tggtaagatc gtggtggttg
    36241 ctggctacgg agacgtaggt aaaggcagtg cgcactctat gcgcggcttg ggtgctcgtg
    36301 tattgatcac agagattgac cctatctgtg cattgcaagc agcgatggaa ggttttgaag
    36361 tgacaaccat ggaagaggca gctccttttg ctgacatttt cgttacggca acaggctgtt
    36421 gcgatatcat cactgagaag catttcagca agatgaaaaa caacgcgatt gtttgcaaca
    36481 tcggtcactt cgacattgag atcgatatgg cttggttgaa caaaaattca aaaatgcgtg
    36541 aaatcaaacc acaagtggat atccacacca tgaaaaacgg ccgccaaatc atcgttttgg
    36601 caaaaggtcg tttggtcaac ttgggttgcg gcactggcca tccaagcttt gtaatgtcta
    36661 acagcttcac gaaccaagta ttggcgcaaa tggagttgtt cttgaaccac gctcaatacc
    36721 aagacatcgc ggtttacaga cttccaaaac atttggacga aaaagtagcg gcattgcacc
    36781 tcgaaaagct cggcgtaaaa ctaaccaagc tttccggcaa gcaatccaag tacctccaca
    36841 tcgacaccga aggccccttc aagcccgacc actaccgtta ctagaaggta cggcctactt
    36901 tctagtagcg cgtcgcgaaa aagcccctgt cttaaaggac ggggcttttt tttgtctttt
    36961 ttaactgaca gaaatttact ttttagggat ttcgataaag cggcagtagg ccttgagttc
    37021 ttgaatgctt tcgcggacgt catcgagggc gcgatgttga ttggatttgc tataagtgat
    37081 gccgaatttt ttattcataa tcactttcca ggaactcaca tcaaccacgc ggtagtgcag
    37141 cctctcgctg aactcaggca tgtatttgtc gataaagagg cgatcttgat tgatcgagtt
    37201 gccagccaag atgggcttat ttcgcgggtc agggaaatgt ttcttggcga gatcgcagag
    37261 cttggcctcg gcaagatcca gggccatgcc atagggaacc ttggcggtaa gtccggactt
    37321 cttgtgatgc tctttattcc agttatccat attatcgagg tagatttgcg gttgtcgtac
    37381 gaccgtttcc aactgatcta actcttgaaa atcaaaatca gtaataatcg cagccacttc
    37441 gatgataacc tcttttttaa catcgaggcc tgtcatttcc atatcaatcc agaacaattt
    37501 ttccattaac tcttctttgc tagtccgaca tcaattgcgt cgcagaccgt ttgaataagc
    37561 ttattttcat cccatggttt gtcaataaaa gcaaaggcgc cgagacgtat agcttcggag
    37621 gccctatcct gatggcgata tcccgaatga ataatgaagg gagtcttcag gccttgctcg
    37681 cgcatccact taaggacctc gagccctgat tttttaggca ttttctcgtc agagagaacg
    37741 gcatcaaatt ctttatgagt gagaatttct atgccttctt tgccattaga ggcttgaacg
    37801 atctccagga catgttcctc aagaagagca atcagcacct ctctcagatc tgcctcatcg
    37861 tcaataataa ggagtcgtcc tcgtttatga tttttcatgg tctaaatcta aatgaatact
    37921 tggctgcaat gcaaggagag taaaatgcgg agataaacaa tttcatttta aggattctga
    37981 gtttttgtga cggaaggcag tggtttagaa gattcagcgg aatgggccga acgggtggac
    38041 agggggtgtg aggtggaggc ctttgtaaga agtttttgtt ctgtttccct cctaaatttg
    38101 cttaaacaga gagcaaaatc cctaaaaatc tgattttatc tattttttaa taaattctat
    38161 gtaaaatttt gttgaatagc gacgttgaga tcagtataaa tgtttcctct atgcggggtg
    38221 gtagttaagt tggttataac gtccgcctgt cacgtggaag gccgcgggtt cgagtcccgt
    38281 ccaccccgcc atttttatag aaccgataaa attaccgagg ctaagtactc ggttttttta
    38341 tgcttttttt ctactttaaa tttctcttct aaatgctaat ttccttttac gggacaatgc
    38401 gggacaggcg gggttgaact cgccccgcca atgcattcct tcgccttctc aggcaatgcc
    38461 agtacggctc tcgcgactcc atccaatgct cttgctactt tccatttccc ctcatccact
    38521 tcctcaacat acttctgagt ctcatctaaa cttgagtgtc ctagattcaa cgaaaccgca
    38581 tgaaaatctc cagtaatgcg atttgcttga gtcgcagaag tcttgcgaat gaggtgagtg
    38641 ccactgacgt aattcactcc gcaaagtttc agtgcgcggt tgtaagcgca gccaatactc
    38701 ttgcgaatca tcggccttcc ctccgaatga aaaaccagcg aagtattcgg ggagcgtatt
    38761 tttttaagaa gtcttaactc ttccaccaga atttccggaa tcaccagaaa ccttacctga
    38821 ccgttcttcg ggcaagattt gatttttggt tcccaacttt cttgatccca aatgatcgtc
    38881 tgctcgatgg tcgcaatact tctctcaagg tccaatgcat cccaagttaa accgcaggct
    38941 tcaccaatgc gaagtccaag tccaaactgt gtcagtgcta gcggaaaata aagaggattt
    39001 ttctgtgctt ttaaagctct tagaaaattc cctaaatcat ctggtcttag tgatctcaca
    39061 ggctgttttg cgcgtcttac aacttttgcg gcttgatagt gttctttgaa aatcggcaga
    39121 ggatagagtg cgttcactct ttttctgtaa taattaagaa ccaccttcaa ggcatccaat
    39181 tccttttcaa atgagaatcg agtttttcgg cgcggataat tctcgtgcca atgtcgaatc
    39241 atttgatcga tcaccttggg actgatttcg tcaacataat atgaagacaa ataattcaaa
    39301 ggtgggagct ttttgtcgta agtttgccgg gtcgtgaaat caattgtcat gtaatgaaag
    39361 tctttccatt cttgcaataa agctgcaaat cgcaatttat ccttagagtc gagagttaca
    39421 ttcttgtggt gccttcccgt ttcgtgcttg gcaagcttgc cgatacgaaa ctctttagcg
    39481 tcctcaaggc tatcaaagaa acaagattct ttttttgaaa gttctccctc gattcgcact
    39541 cggcgtatcg atcggtactt tccggaatct tgccatcttt taagcttttc attccaatcc
    39601 aacaatttat aaattccagg ataatttttt agcgcttctt cctttttctt tttcatctgg
    39661 tgccaccttt gtggagccat tcagaaattc gttgaggaga gaagcgaacc agtcttccta
    39721 cttttaaata aggaattcta tttttcgaaa ctaactttct cacatggcga atagaacagc
    39781 taagttcctt tgccacatca tcaatcgtcc ataccaactt attcgttttt tcaaagagcg
    39841 agggtgaatt gtttttcacc cttgatgaat ctattctcat gtaagtctcc tggaaaatca
    39901 atcaaagaat tagtttgcgc gcagattcga catcattaat atccgcgagg ggaatggatt
    39961 ctggcgagtt tgatggagct tcgctcatgt cttttttaga acgtttcttg cgggtttttg
    40021 gtggtgtcac catggtctcg tgtctgtcgg caatctttgt gtcagcatgt tgaattacgg
    40081 caagctcaat gcttcggatt tcctcctgaa tttcagcaac tttttcagtg tctccattta
    40141 tcagagcctc tttgagacga ggcgtaatcc aattaagatg cttgatagga tcgtagtgat
    40201 agaattttat cttttgcatt tctttggtcg tgagttccga tttgaactcg cgaataagaa
    40261 aatttacgac atccgatttt gacagtgcta aaaatccttt tgaggaactg ttgatctgtt
    40321 caagccatcc ttgggctttc gtggcttcat cagagcctag gcagattctc tcagtcgcct
    40381 tgggtttgtt tgtcggtgta ataatattct cagttagcga ctccatgttg tctccttctc
    40441 tattcggaac aggtagtgac cttcctccca ttcggccccg ccatttttaa ttactggagg
    40501 aatccatatc ttctgaacga cgggtttcaa taaactcggt ggagggtttt taatgtcatt
    40561 tgttggaact tttttattac tggaaacaca cgcatttagg gtgagacaaa atgatagaaa
    40621 taacgagaag actcgtttca tagacgggct cctttgattg tgggtaattg tgtgagaata
    40681 ggtcaggggg tatacagtca gagattcatc cctgaatcag tgtggttctt aagtctttga
    40741 ttttccgatt actttcgtcg gatggtaatg cgcggtaact ctattcagct cccgtgaagt
    40801 accgaggata tccccgtaaa aatccccgag aagtccccgt aaattctcgc gcaaggtgaa
    40861 gagggtttct gaccgttggt tgattgctac agcgttgaga cctttttcca tatattcttt
    40921 caagtccaca aagaggtgga tgttttccga agggtctttt acgcaatcct tagcccgaag
    40981 aatatttgcc atgattaaag gatctgacac cagccagaga acccggtcca cctggcgttg
    41041 atggcgataa aactgaataa cattttcata gcgactggtg ctcttcggtg aaagctcgac
    41101 ttcaatggca taggtcacag ccttttgcag tccaagcacg cgggtgtatc catccggtcg
    41161 atggtctttg gtttttggaa tccaatccgg gtattggtcc ttggagcacc gtcgcagttc
    41221 ctgctcagag aaaaacagaa tgcgctccgg tcgtctcccc agccactcgc ccaaatgaaa
    41281 agccaaaaca tgcctgtcgt gtttgtgatt ttcagataaa taaccgtctt cttcgagttc
    41341 aggtaagttt agcttaatag attcaaaacc cttttcgcga agctgccacg cccagaaaat
    41401 ctcagtgtca ttgaacttag cttcgacgta
//
LOCUS       DATBTE010000284         3932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1334611, whole genome shotgun sequence.
ACCESSION   DATBTE010000284 DATBTE010000000
VERSION     DATBTE010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334611"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1380)
                     /locus_tag="VIG33_10560"
     CDS             complement(85..1380)
                     /locus_tag="VIG33_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271318.1"
                     /translation="MKFNSKIIEQKSFYTWLTLVFLFGQWVLIALTSVNVPAGDEWEN
                     LNAGALPSGFSFEYLFAFHNEHRIVFTKLLTYAFFYLTHWNLSYHVIFNYTLFAAVVL
                     FFMFFQKKFISQSSKGLWILPFFLTSPLLIDNHNWAIQSCFHFCVLFGVLGIFFATRE
                     HLRNIHFWLAGLLGLGSMYSLAAGAFFTLTVLFVLLYRLLVGPRKSLSEWALQTAAVA
                     ALIAGIGAWFIGFHSPSEQPVFTWPYKIDFWYFYANLISLGFGFKTSSAIIAALALGI
                     VAFVLGKSFKAAFSFKQHFVSLGFFGSLAVLGAFGSIALARTGYGIGQAKTSRYAELG
                     VLLVLYIGWLLWKMAQETPKYQRLFKYFIWFVFIGFLGDYSYPKYFSVAEERQEGLRC
                     ISKYYSGENKTGDCPVLYPGPLAERLERAKAMKLSWVPF"
     gene            complement(1370..3265)
                     /gene="asnB"
                     /locus_tag="VIG33_10565"
     CDS             complement(1370..3265)
                     /gene="asnB"
                     /locus_tag="VIG33_10565"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEY8271319.1"
                     /translation="MCGIFGLIGSRDKEEIKTANRSIAHRGPDAEGYWFDDENSVALC
                     HRRLSILDLSELGAQPMQSESGRYVISYNGELYNFLELKTKLQKTNWRGTSDTEVLLA
                     CFEEWGLEKSLDSIEGMYAIALWDTQLKTLTLIRDRLGEKPLYYGVVQGVFTFASELK
                     PFISLFKKDLSINPLALEDFFKRSCISGDKSIFKGIHKLPAGHFVIIPLQSLRDKKAL
                     SESKKYWALQDLSLGTWTLSREEALQQLEGKLHKAIQQQMIADVPLGAFLSGGIDSSL
                     IVALMQSLSPKKIKSFSIGFSSHHHNEAPHAKAVAAHLGTEHEELYVSEADLLAQVPL
                     LASIYDEPFADSSQIPTILVSQMARKYVKVALSGDGGDELFGGYNRHAFAEQHWGKVR
                     LIPAPLRSVVAKTLTCLPPSAWESVFKLISSGASQPHEKIYKLAEVLKSSSMSDFYRS
                     VSTHWNDTSPLLNKNKQVNQHNQDWDVHSALEMSLADAKWYMPDDVLVKVDRAAMSQS
                     LETRAPFLSKEVVEMAFNLPIDFKIYQGQTKWILRQILYKHVPKELIERPKMGFGVPL
                     DSWLCKELKEWAWSLLSPQALNQQDLLDAKTIHQKWDEHQRGLRNWQTEIWDVLMFLS
                     WRENYEI"
     gene            complement(3271..>3932)
                     /locus_tag="VIG33_10570"
     CDS             complement(3271..>3932)
                     /locus_tag="VIG33_10570"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY8271320.1"
                     /translation="AMVVLSLTRGIWIGFAFALLFCAGFLGRKVFASSVLALVLTAGL
                     LFYGSRRIQNRVMGKTNAESQSNNERMLYWKANLQIFKDYPLWGIGYSQNNIHVEEYL
                     KKEGVEGLVGTHAHNQYLHFLAGTGLLGLACFLAFLALVFYSLLRRLWQWRKQKSSSD
                     QYYLLLGAFAAILCFAIGSLTESNFSIAKNRFAFLFIAALGYAMSTSKISANSANPSK
                     K"
BASE COUNT         1229 a          957 c          794 g          952 t
ORIGIN      
        1 ggcaggtctt ctcacttaaa aatgaccgcc gatgaaccgc aaatctttat ctcggatatc
       61 cagcaataag cttcggcttg agccttaaaa aggcacccac gaaagtttca ttgcttttgc
      121 cctctccaaa cgctccgcca gaggtcccgg ataaagcacc ggacaatctc ccgttttatt
      181 ttccccggaa taatattttg agatgcagcg aagaccttct tgtctttcct ctgcaacgct
      241 aaaatatttt gggtaagagt aatcgcctag aaatccgata aaaacaaacc agataaaata
      301 cttaaaaagc ctctgatact ttggtgtttc ttgcgccatt ttccataaga gccaaccaat
      361 atataacacc aacaatacac ccagttccgc atagcgagag gtttttgcct ggccgatgcc
      421 ataacccgtc cttgctaagg cgatagatcc aaaagccccg agtacagcta aagatccaaa
      481 aaaacccagg gatacaaaat gctgtttgaa tgaaaatgcg gctttgaagc ttttccccag
      541 aacaaaagcc acgatcccta atgctaaggc cgctataatg gcgctcgaag ttttaaaacc
      601 gaatccaagt gaaatgagat tcgcataaaa ataccaaaaa tctattttat aaggccaagt
      661 gaagaccggc tgctcgctag ggctatgaaa gccaataaac caggccccaa ttcctgcaat
      721 gagagccgcc accgcggctg tttgcaaagc ccactccgaa agggattttc gcggccccac
      781 cagtaatcta taaagcaaaa caaataagac cgttagggta aaaaaggccc ctgccgccaa
      841 tgaatacatc gagcctaagc ccaaaaggcc cgccaaccaa aaatgaatgt ttcgtaaatg
      901 ttcgcgggtc gcgaagaata tccctaggac tccgaacaaa acgcaaaaat gaaaacaaga
      961 ttgaatcgcc cagttatgat tatcaatcaa aagcggcgaa gtcagaaaaa aaggcaatat
     1021 ccagaggccc ttgcttgatt gagaaatgaa ttttttttga aaaaacatga aaaacaaaac
     1081 gacagcggcg aaaagcgtat agttaaatat aacatgataa gaaagattcc aatgggtcag
     1141 ataaaagaaa gcataggtga gcagtttagt aaaaacaatc ctatgttcat tatgaaaagc
     1201 aaaaagatat tcaaaactaa atccactggg aagcgccccc gcattgaggt tctcccattc
     1261 atctcccgcc ggcacattca ctgaagtcag agcgatgagg acccattgac caaaaagaaa
     1321 aaccaaggtc agccaggtgt aaaagctttt ctgttcaatt atttttgaat taaatttcat
     1381 aattctcccg ccaagataaa aacatcagaa catcccatat ttccgtctgc caattgcgca
     1441 gcccccgttg atgttcatcc cacttctgat gaatcgtctt cgcatctaaa agatcctgct
     1501 gattgagtgc ctggggactg agaagactcc acgcccactc tttaagctct ttacacagcc
     1561 atgaatccag cggcacacca aaacccattt tgggacgctc aatgagttct ttggggacgt
     1621 gcttgtaaag aatttggcga aggatccact ttgtctgccc ttgatatatt ttaaagtcga
     1681 tcggtaagtt aaaagccatc tccacaacct ctttactgag aaagggcgca cgggtttcca
     1741 gactctgtga catagcggct cgatcgactt taaccaagac atcatccggc atataccact
     1801 tggcatccgc aagactcatc tcaagagctg agtgcacgtc ccagtcctga ttatgctgat
     1861 ttacttgttt atttttattt aacaagggtg aagtgtcatt ccaatgagtg gaaaccgagc
     1921 gataaaaatc agacatgctc gaagacttta ggacctcagc cagtttataa attttctcat
     1981 gcggctgtga agctcccgaa gaaatgagct taaacacaga ctcccaggcc gaaggcggaa
     2041 gacaagtcaa agtcttcgcc accacgcttc ttaaaggggc aggaatcaaa cggacttttc
     2101 cccagtgctg ctcagcgaag gcatggcgat tgtaacctcc gaagagttcg tccccgccat
     2161 cccctgacaa agccactttg acatacttac gagccatttg gctgactagt atggtgggaa
     2221 tctgcgaaga gtccgcaaag ggttcatcat aaatacttgc gagcaatgga acttgggcca
     2281 agagatccgc ttccgacaca taaagctctt cgtgctcggt gcccagatga gcggcaacag
     2341 cctttgcatg cggagcttcg ttgtgatgat gactgctaaa gccgatggaa aaacttttta
     2401 tttttttcgg gcttaaggat tgcatcagtg ccacgatcaa agaactatca atgcccccag
     2461 ataaaaaagc gcccaaggga acatctgcga tcatctgttg ctggatcgct ttatggagtt
     2521 tgccttccaa ctgctgaagc gcttcttctc gagacagcgt ccatgttccc aaagaaagat
     2581 cctgcaaggc ccagtatttc ttggactcac tgagagcttt tttatctctc agagactgaa
     2641 gggggatgat cacaaagtga cccgccggaa gtttatggat tcctttaaaa atactcttat
     2701 cgccagaaat acaacttcgc ttaaaaaaat cttcaagagc caagggatta atagaaagat
     2761 cttttttaaa tagacttata aaaggcttta actccgaggc aaaagtaaaa accccttgaa
     2821 ccactccata ataaagaggt ttttcgccca ggcggtctct gatcaaggtt aaagttttta
     2881 actgcgtatc ccaaagagcg atggcataca tgccctcaat actatccagg gatttttcaa
     2941 ggccccactc ttcaaaacaa gccaataaaa cttcagtatc cgaagtgccg cgccaatttg
     3001 ttttctgtag ctttgtcttg agctcgagaa aattataaag ttcgccattg taagatatta
     3061 cgtagcgtcc actctccgac tgcataggtt gcgcacccaa ttccgaaaga tccaatatcg
     3121 acaatcggcg gtgacaaaga gcgaccgaat tttcatcatc aaaccagtag ccctccgcat
     3181 ccggcccgcg atgagcgatg ctacggttag cggtctttat ttcttcctta tcacgactcc
     3241 caatcaatcc aaaaattccg cacatagttc ctacttctta gagggattgg ctgagttagc
     3301 ggatatttta gatgtactca tggcatatcc caaagccgcg ataaataaga atgcaaaacg
     3361 gttttttgca atgctaaaat tagattcggt taaagaacca atggcaaaac agaggatggc
     3421 ggcaaaagcg cccagtaaca aatagtactg atcagaactg cttttttgtt ttcgccactg
     3481 ccaaagacgc cgcaagaggg agtaaaaaac caaagccaaa aaagccagaa aacaggccaa
     3541 tcctaaaaga cccgttcccg ccagaaaatg aaggtattga ttgtgtgcat gggttcccac
     3601 caacccctcc acgccctctt tctttaagta ctcttcgacg tggatattgt tctggctgta
     3661 accaatgccc cataaaggat agtctttaaa aatttgcagg ttggctttcc aatacagcat
     3721 tctttcgttg ttactttgag actccgcatt ggttttaccc atcactcgat tttggatacg
     3781 acgggagcca taaaaaagaa gccccgccgt taaaactagg gccagaaccg aactggcaaa
     3841 aacttttctt cccaaaaagc ccgcacaaaa caaaagagca aaggcaaatc caatccaaat
     3901 accgcgggtt aagctgagaa ccaccatggc ac
//
LOCUS       DATBTE010000285         9476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1608721, whole genome shotgun sequence.
ACCESSION   DATBTE010000285 DATBTE010000000
VERSION     DATBTE010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 9476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9476
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608721"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2215
                     /locus_tag="VIG33_10575"
     CDS             <1..2215
                     /locus_tag="VIG33_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271321.1"
                     /translation="INSNMDEKYFNGSGNYNGYVQPKIEIAPLEFKFLRVGKDTSDRE
                     IIYNIKACFKNGLDQKITRGFTFNVSGFQQAESETANAPLKTVALAADNSSCINWDET
                     LTFKYYECHKYLKGSVQIENKDLALKQKIEVAINPWEPAGTFARDLRYVDNQESLVNE
                     CKKESILPAMISLKTISYSTLSNDYEIDHLLNLTVKKKLRFKIDSEVNIFSDMSKGRV
                     ENAQRLRPGIYLLKLALVKNRDYYNQKTYITSVEKLVATANGDLRGDIEFKTADLKAL
                     KSHDTLLLEIDPVKEDKVTVDPQGNLVAKEKVTSLDEIIDRSTGLYSRTFSTVMGLGD
                     KSGFLELNPVESKDINQYFTNANLPPPAESGKSMIREYIKYGEKLASDNLQAQRAQAD
                     ITLFAKNNSLRQLSLKEPQSSEHLRAALAKSLPRLNNTEIKNLLSQFATTGKLDTSLS
                     KGLCSYWFHNFIVNELDGFFKYHEYIKCDLRANHPEQIFTVEKRLFVKELESYRFLNG
                     YTYPISVGNSITLTKSESHSTSISKSLGLSLNMIPFEFAKIFSIGISANYAISHSDSE
                     DEGNANSVSIGSNIPLSMEQNVYQLKFKRYQECASIKINPQHFLKKGLFSNSLKPKLK
                     ESEKAEIASRGLMICTGVDNTTPLVRNENYYQLNQAPGDTQIQDNGDLRNIHFFMALR
                     GEKEFQRLMYFMKGNIKTPQTADRSSDEQKNTFTNLNNLFNTGFTNVPGSYNDTH"
     gene            complement(2225..2962)
                     /locus_tag="VIG33_10580"
     CDS             complement(2225..2962)
                     /locus_tag="VIG33_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEY8271322.1"
                     /translation="MRIVSMVPSWTETLLRAGVSVVGRTRFCIHPTELVKNIPVVGGT
                     KEAQWDLIKDLQPDLVLLDKEENPLEMAQECPCPYLATDVHSLETLQFELQRLGAELK
                     NPKLITWSLQLQMILDRGPLSWNEENIPGLIEWVISPMRPGPMPVLYMIWKTPWMAIS
                     RETYIGSVLTQLGAQVVNFPEDKYPVVKIQDFQEALILFSSEPYPFHKKIEDLRREGL
                     AGAVVDGEAYSWFGIRSLEFLKKCYGH"
     gene            3073..4365
                     /locus_tag="VIG33_10585"
     CDS             3073..4365
                     /locus_tag="VIG33_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271323.1"
                     /translation="MNPTAFLKRKPHLSPYKKGDVLVLFGELFQRGYANGLVEEAESR
                     GMTIVYATVGRREAGQLRTLNAEELKNVPKPFINIPLEAGFDLEPDSQGHAPVDQLKE
                     VKLSEWENVKINFASLEESRQKAVARFRKNVEAFLQELESHIPTGANLVFAHLMAGGV
                     PRTKIVMPLMNRTCKGSGDRYLSSEQFWNSDIGKFCALNFKEVTAETLRHLVELSTPL
                     RQKLEAKGSHVAYTAYGYHGTEIMIDGQYQWQTYIPYIQGWAKLDLEKIATEAHKKGI
                     NICVYNCPEILTNSSSIFQGVEVSLYPLTKAIEKECGTSVAGKKILADCKALLKENVS
                     LSDIEEIANSYFVDPEIKAHCIFKAWPQHSSKQQLEIMLTASDKLIWLHKDSKKLITF
                     ELSEVVFKSCGYVMLHDSWKPQSPVAWIGHELVARCIQ"
     gene            4365..5207
                     /locus_tag="VIG33_10590"
     CDS             4365..5207
                     /locus_tag="VIG33_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8271324.1"
                     /translation="MDLSNGANYSYAGLNLKGFSLSGIRTAISVPDLSLTFDVAQGYP
                     FLLNLKQFFLSHGHLDHAAGVPYIISQKAMNHQPPPQFYMPQSLVEPLDHIMKIWQQI
                     EGHEYKYDFIPVKADLEIPVKGNYFVKVFPTKHRIDSFGYTLFEFHKKLSPKYQGLSQ
                     DELMKLKKEGHPLNESIRTPVLSFTGDTQIEFLHSRPWVKHSKILLLEATYLDDRKPI
                     EHARTWGHTHLHEILSRLEEIESEKIVLIHTSSRYTWEEAQKLVYQQVPAKHRHRVEL
                     FPGR"
     gene            complement(5214..5927)
                     /locus_tag="VIG33_10595"
     CDS             complement(5214..5927)
                     /locus_tag="VIG33_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271325.1"
                     /translation="MNNIAVRFYIRFIIFSFVFYTTFVTSAKAMAEDKVVIGYFSTEN
                     QETFEKNVKPAFERLNGACKKCEIMNLTPYDEKGVYSEKDLLEKLKNSPPEISFYFFS
                     WNKKATDRNKELISALGEKIEGGKLVIAPTGQAAEGEPGLPLSRTLMGQTKDVIIIGE
                     IIGNERMLPQSYFGPEMLTALRAPKDLAVQGMGPMYFVGRLANNWSKRKPEEWLQHFK
                     TKKIKSRKIWLDADDLFGR"
     gene            6064..7170
                     /locus_tag="VIG33_10600"
     CDS             6064..7170
                     /locus_tag="VIG33_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HEY8271326.1"
                     /translation="MELISGIRRAQKSITLEFYIFEIDQVTEILFSELQMAVLRGCQV
                     HLLVDGVGSFFWLEALSKRCQNEGIHFRIYHPLPGFLQWFAHALEALFAKTPKVLRRI
                     NRRNHRKTVVIDQQVAFLGSFNMTKVHYAMLVGANKAWRDSGMKIQGEAIESLIAAFD
                     LAWTFARKKALFPTHDKYIREKIQTVLRWQSLLRLNANDKIRRHLYKDLCRRILHAKS
                     RVYIATAYFLPKRAIARALTKAAQRGVDVRILIPGPSDIPLVKWAAHQLPYKLHLGGV
                     KIFEFQPRVFHAKYMIIDEWSSLGSFNLNHRSLLHDLEVEAVLTDAESLCNMNTRFEQ
                     DLKECQPLELARYQKVFFLKNWITKTLFKLRYWF"
     gene            7203..7808
                     /locus_tag="VIG33_10605"
     CDS             7203..7808
                     /locus_tag="VIG33_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8271327.1"
                     /translation="MKLIKVTVTGGPSGGKTTLIEALQKGMGKTVSVVPESATILYRG
                     GFPRIKSHNGRIHAQKAIYYTQKELEDLISMEQPTKLLVCDRGSMDALAYWPETPQSF
                     FKVINSTPEKEYSRYDWVLHLDTASAEHYDSTNPVRTESYSEAQSLNQRILDAWGMHP
                     QRVRIGEKKDFLAKMAAAYGVIQAILKGEDLQIIRKNFELI"
     gene            7834..>9476
                     /locus_tag="VIG33_10610"
     CDS             7834..>9476
                     /locus_tag="VIG33_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271328.1"
                     /translation="MFTINLMVNTIRFLIFVVISVIIFQTILSPRASAASFENGTPLL
                     FQADKNNIQILPQRFEYNLIDEDQLKIGDILINYVTFGFQILPSANFPGKYRAKFTWP
                     SSLLQEGSLLIKNNTGKAVWTANFSRRSVRLVEPKTNEQNKLQNKNLRTQVAEMVIDQ
                     LNPDLIEDMKYFPFMTFCIANTTQETRIYFCSKELYLTSKDNHLLVKARSQEKRTPLV
                     EVNGKSVGNQGIIFLNNESENIGFRAVSQSGAYLEVETRMKPVDFKDIILSEDKKEII
                     LTASGAEPVNEESIQRLSENEWRVKLDASRPILYLKGEGNIPMRQEFYIKGNIPSEQA
                     RPSLEKDSFDHIYKSEINLSGTFASGTSVSATTENEDVEKTDFNKFRWHLTEIPGGET
                     SRHYLRVQHKENSYLAAYDIYRDYSFESALWGTYWSHSGLFADISLNWWMENFLTSEA
                     LWARLHWGINFKESLRLTKKTDETSVTISHLELLWRANPGFHFQDRTWGLSLPYEMIQ
                     GTGMNISSLGLGIFSSSKPPKKYQRYLHWYDLKMNYLMGG"
BASE COUNT         2818 a         2231 c         1904 g         2523 t
ORIGIN      
        1 tataaattca aacatggatg aaaaatactt taacggctct ggaaactaca atggctatgt
       61 ccagccgaag atcgaaatcg cgcccttgga atttaaattt ctccgtgtcg gaaaagacac
      121 ctcggatcgg gaaattattt acaatatcaa agcttgcttt aaaaatggcc ttgatcaaaa
      181 aatcactcga ggttttactt ttaatgtcag cggcttccag caagccgaaa gtgaaaccgc
      241 aaacgcgccc ctcaagactg tagcgctcgc agccgataat tcctcctgta ttaattggga
      301 cgaaaccctg acttttaaat actacgaatg ccacaaatac cttaaaggtt ctgtccagat
      361 tgagaacaaa gatctcgcat tgaaacaaaa aatcgaagtc gccatcaatc cttgggaacc
      421 cgcaggcaca tttgcccgcg acctcagata tgtggacaat caagaaagtc tggttaatga
      481 gtgcaaaaag gaaagtattt tacctgcgat gatttcattg aaaacgatct cctactcgac
      541 tctctctaat gactatgaga tagatcacct gttaaatttg acagtcaaaa agaaactccg
      601 ctttaaaatc gattcagaag taaatatctt tagtgacatg agtaaggggc gcgttgaaaa
      661 cgctcagaga ctgcgccccg gaatctattt gctcaaatta gctttagtta aaaaccgcga
      721 ttactataat caaaaaacct atatcacttc agttgaaaaa ctcgtcgcca ctgccaatgg
      781 cgacctccga ggggatatag aattcaaaac agccgacctc aaagccctta aaagccatga
      841 caccctcttg ctcgaaatag atcctgtgaa agaagacaag gtgactgtcg atccccaggg
      901 caacttggtg gcaaaagaaa aagtgacttc actggatgag atcatcgatc gcagtacggg
      961 actttacagt cgcaccttta gcacagtcat gggtttgggc gataaatcag gtttcttaga
     1021 acttaatccc gttgagtcta aagacatcaa ccagtatttc accaacgcaa acttgccgcc
     1081 ccctgctgaa agcggtaaat ccatgatccg agaatatata aagtatggcg agaaactggc
     1141 ttccgataat ctccaggcac aaagagctca agcggatatc accctttttg ctaagaacaa
     1201 ttctttaaga caactctctc tcaaagagcc tcaaagctct gagcatctcc gcgcagccct
     1261 cgcgaagtcc ctgcctcgcc tgaataatac tgaaattaaa aatctattaa gccaattcgc
     1321 aaccaccggg aagttggaca cgtctttaag caaaggcctt tgctcatact ggtttcataa
     1381 ttttatcgtc aatgagctcg atggattttt caaatatcac gagtatatca aatgtgacct
     1441 tcgcgcgaat catcctgagc aaatcttcac cgttgaaaag cgccttttcg tgaaagaact
     1501 cgagagctac cgctttttaa atggttatac ctaccccatc tccgttggca atagtatcac
     1561 tttgacaaag tccgagtctc attccacttc catttccaag tccttgggct taagcttaaa
     1621 tatgatccct tttgaattcg caaaaatatt ttctattggc atttcagcga actatgcgat
     1681 ttcccactcc gattctgaag acgaaggcaa cgccaatagc gtttctattg gctcgaatat
     1741 tccactaagc atggagcaaa atgtctatca gctgaaattc aaacgctacc aagaatgtgc
     1801 ttcgattaaa atcaatcctc aacacttcct aaaaaagggc cttttttcta attcgttgaa
     1861 accaaagctc aaggagtcgg aaaaagcgga aatcgcttcc cgaggactca tgatttgcac
     1921 cggcgtggac aataccactc ctctggtgcg caatgaaaat tactatcagc taaatcaagc
     1981 cccaggtgac acgcaaatcc aagataacgg cgatctcagg aatatccatt tcttcatggc
     2041 cctccgcgga gaaaaagaat tccagcgttt aatgtatttc atgaaaggga atatcaaaac
     2101 accgcaaacg gcagatcgtt ccagcgatga acaaaaaaat acttttacga acttaaataa
     2161 cctctttaac acaggcttta cgaatgtacc gggaagttac aacgacactc attaattaat
     2221 atgattaatg cccgtaacat ttctttagga attccaagct ccgaatgcca aaccaagaat
     2281 acgcttctcc gtcaacgacg gctcccgcga ggccttcgcg gcggagatcc tctatttttt
     2341 tgtgaaaagg atagggctcc gatgaaaaca aaatcagggc ctcttgaaaa tcttgtattt
     2401 tgacaaccgg gtacttgtct tcagggaaat tgacaacctg agctcccaat tgagttaata
     2461 ccgaaccaat ataggtttca cgactaatgg ccatccaagg tgttttccaa atcatataga
     2521 gcacaggcat gggccccgga cgcataggtg aaatcaccca ttcgatcaaa cccggaatat
     2581 tttcctcatt ccaagataaa gggccacgat ccagaatcat ttgcagttgt aaagaccagg
     2641 tgatcaattt tggatttttt aactctgccc ctaaacgttg caattcaaat tgcaaggtct
     2701 ctaaagagtg aacatccgtg gcaagataag ggcaggggca ttcctgggcc atttccaagg
     2761 ggttttcttc tttatccaat aggactaaat caggctggag gtccttgata agatcccatt
     2821 gggcttcttt ggttccaccc acgaccggga tgtttttaac tagttccgta gggtgaatgc
     2881 aaaaacgcgt tcttcccacc actgaaaccc cagcccgtaa taatgtttcc gtccatgagg
     2941 gcaccatcga gacaattcgc atcttttgat accaccaatt attgcttgtc gagtcaagat
     3001 tggtatattg tcacttgacc cattccttat cactaagcga aaaatagttt ttacctatga
     3061 aggaattcaa ctatgaaccc tactgcattt ttaaagagaa agcctcacct cagcccttat
     3121 aaaaaaggcg atgtcctggt cctctttggt gaacttttcc aacgaggtta tgccaacggt
     3181 ctggtagaag aggctgaatc ccgtgggatg accatcgtct acgccactgt cggtcgccgt
     3241 gaagctggcc aactccgaac tttaaatgcc gaagaattaa aaaatgttcc aaaacccttt
     3301 atcaatattc ccttagaggc cggcttcgat cttgaacctg attctcaggg gcatgctccc
     3361 gtcgaccaac tcaaagaggt caaactttct gaatgggaaa atgtaaaaat caactttgcc
     3421 tctctcgaag aaagtcgtca aaaggcagtg gcgcgctttc gtaaaaatgt tgaagcattt
     3481 cttcaagagt tggagtccca cattcccacc ggagcaaatc ttgtttttgc acatttaatg
     3541 gcaggaggtg tgcctcgcac taaaattgtc atgccgctga tgaatcgtac ctgcaaaggc
     3601 agtggcgatc gttatttgtc ttcagagcaa ttttggaact cagatatagg caaattctgc
     3661 gccttaaact ttaaagaagt tacagccgag actcttcgcc atttggtgga gctttccaca
     3721 ccgcttcgtc aaaaactaga ggctaaaggc tcccatgtgg cctatacggc ttatgggtat
     3781 cacggcactg aaatcatgat tgatggtcaa taccagtggc aaacctatat tccttacatt
     3841 caaggctggg caaaattgga tcttgaaaaa atagccactg aggctcacaa aaaaggcatc
     3901 aacatctgcg tttataactg tcccgaaatc cttacaaact caagctccat ctttcaggga
     3961 gttgaagtat cactttatcc cctcaccaaa gccatcgaga aagagtgtgg aacctctgtt
     4021 gctggaaaaa aaatccttgc tgactgtaag gccctcttaa aagagaatgt cagcctgagt
     4081 gatattgaag agattgccaa cagctacttc gttgatcccg aaatcaaggc ccattgcatt
     4141 ttcaaagcgt ggcctcaaca tagcagcaag caacagcttg agatcatgct cacagcctcg
     4201 gacaagttaa tctggcttca taaagattct aaaaaattga ttacttttga actcagtgaa
     4261 gtggtgttta aatcttgtgg atatgttatg ttacacgact cctggaaacc acaaagtccc
     4321 gtagcttgga tcgggcatga acttgtggca cgctgtattc aataatggat ttaagtaacg
     4381 gagctaatta cagttatgcg ggtctcaacc tgaaaggctt ctctctttca ggtattcgca
     4441 ccgctattag tgtcccagat ctttccctta cctttgatgt cgctcagggc tatccctttc
     4501 ttctaaatct aaaacagttc ttcttaagcc atggtcatct ggatcatgcg gccggtgttc
     4561 cctacatcat ttcgcaaaaa gccatgaatc atcagccccc tccccagttt tacatgccac
     4621 agagtctcgt agagccttta gatcacatta tgaaaatctg gcagcaaatc gaaggtcatg
     4681 aatacaaata tgatttcatc cccgtgaaag cagatctgga aattcctgtg aagggaaatt
     4741 attttgtaaa agtcttcccc accaaacatc gcattgattc gtttggctac acccttttcg
     4801 agtttcacaa aaaactttcc ccaaaatacc aaggcctttc tcaagatgaa ttgatgaaat
     4861 taaaaaaaga gggccatccc ctcaatgaat ccatcagaac tcctgtgcta agctttactg
     4921 gggatacgca aattgagttt cttcacagtc gcccctgggt taaacactcc aaaatcctat
     4981 tactagaggc cacgtatttg gacgacagga aacccattga acatgcaagg acttgggggc
     5041 atacacattt gcatgaaatt ctatctcgcc ttgaggaaat agaaagtgaa aaaatcgttt
     5101 tgattcacac ttccagccgc tatacttggg aagaggctca gaagttagtt tatcaacagg
     5161 ttcctgcaaa acatcggcat cgggttgaat tatttcccgg aagataatta tttttatcga
     5221 ccgaataggt catcggcatc taaccagatt ttccggcttt tgattttctt tgtcttaaag
     5281 tgctggagcc attcttcagg cttacgttta ctccagttat tcgcaaggcg accaacaaaa
     5341 tacatcgggc ccatgccttg gacagccaaa tccttcggag cgcggagggc ggttaacatc
     5401 tccggtccaa aataactctg aggcaacatt cgttcgttac caataatctc gccaatgata
     5461 atcacatctt tagtttggcc catcagggtg cgactcaaag gcaatccagg ctccccttca
     5521 gccgcttgac ccgtgggtgc aatgaccaac ttaccaccct cgatcttttc acccagggcg
     5581 cttataagtt ctttgtttcg gtctgtagcc tttttattcc aagaaaagaa ataaaaagat
     5641 atttctggcg gcgaattttt cagcttttcc agaagatctt tctcagagta aactcccttt
     5701 tcatcatagg gagtcaaatt cataatttcg cacttcttgc aggctccatt aagccgttca
     5761 aaggccggtt ttacattttt ctcaaaagtc tcttggtttt ctgttgaaaa ataacctata
     5821 acaactttat cttcagccat tgcttttgca gaagtaacga aagtagtgta aaacacgaac
     5881 gaaaagataa taaaacgaat ataaaaacgc acagcgatat tgttcataaa aatattatga
     5941 actcgacttt ttagggatgt cgaccaaaat atcattcaac attgcatcat ggctaaaaca
     6001 tggttaaaca tgataaaaac ctgggatcga gttcagcttt ttcatagtgg ggatgagttt
     6061 tttatggaac ttatctctgg tatccgacgg gcgcaaaaaa gcatcacgtt ggaattctat
     6121 atttttgaaa tcgaccaggt tacggaaatt cttttctccg aacttcagat ggccgttttg
     6181 cgcggttgcc aagtccatct tttagtagat ggagtgggct catttttctg gctcgaagcc
     6241 ctcagcaagc gctgtcagaa tgaaggtatt cactttcgta tctatcaccc cctccctgga
     6301 ttcttgcaat ggttcgccca tgcgctggaa gccctctttg ccaagactcc aaaggtcctt
     6361 cgcagaatca atcgccggaa tcatcgcaag accgtggtca ttgatcagca agtggcattt
     6421 cttggcagct ttaatatgac gaaggtgcat tacgcaatgc ttgtcggagc aaacaaagct
     6481 tggcgtgact caggaatgaa aattcaagga gaggctatcg aaagcttgat tgcagccttt
     6541 gatctggctt ggacctttgc ccgtaaaaaa gctcttttcc ccacccatga caaatatatt
     6601 cgtgaaaaaa ttcaaacggt tttaagatgg cagagtctct tacggctcaa tgccaatgat
     6661 aaaattcgca gacatcttta caaggatctt tgtcgacgca ttcttcatgc gaaaagccga
     6721 gtgtatattg ccacagccta ctttttaccg aagcgagcca tcgctcgagc gctgaccaaa
     6781 gccgcgcaac ggggagttga tgtccgcatc ttgattccgg ggcccagtga cattcccctg
     6841 gtaaagtggg ccgctcatca acttccctat aaacttcatc tgggaggcgt aaaaattttt
     6901 gaatttcaac ctcgggtttt tcacgcgaaa tatatgatca ttgatgagtg gtccagtctg
     6961 gggtctttta acctcaacca tcgcagtctt ttacatgacc tcgaggtcga ggcggtgctg
     7021 acagatgctg aaagtctctg taatatgaat acgagattcg aacaggactt aaaagagtgc
     7081 cagcctctgg agctggctcg ttaccagaag gtgttctttt taaaaaattg gattaccaaa
     7141 actcttttca agttaagata ttggttctaa actccacaca gacttccaca tagagaggaa
     7201 atatgaaatt gatcaaagtt actgtgactg gaggtccttc tgggggcaaa accactctta
     7261 tcgaagctct gcaaaaagga atggggaaaa ctgtgtctgt ggtccctgag tccgcaacaa
     7321 tcctttatcg cggtggattt ccacgcataa aatctcacaa tggacgcatc catgctcaaa
     7381 aggcgattta ttatacccaa aaagagctgg aggatttaat ttccatggaa caaccgacaa
     7441 aacttttagt ctgcgaccgc ggatcaatgg atgctctggc ttactggccc gaaactccac
     7501 aaagcttctt taaggtcatc aactccaccc cagagaaaga gtactcacgc tacgactggg
     7561 tcctgcacct tgataccgct agcgccgaac actatgactc aaccaatccc gtaagaacag
     7621 aatcttattc cgaggctcaa tcgctgaatc agcgcatcct tgatgcttgg ggaatgcacc
     7681 cccaaagagt ccgaatcggc gagaaaaaag acttcctggc caaaatggcg gcggcttatg
     7741 gggtcattca agcaatcctc aaaggggagg atctacaaat cattcgaaaa aacttcgaat
     7801 taatttgata aatgatttac aagccagaca cccatgttca caataaatct tatggtgaac
     7861 acaatacgat ttcttatttt cgtagttatt tctgtaatca ttttccaaac catcttgtcc
     7921 cccagggcct ctgcggcttc tttcgaaaat ggaactcccc tgctcttcca agctgataaa
     7981 aataatattc agatattacc tcagcgcttt gaatacaatt tgattgacga agatcaactt
     8041 aaaatcggtg acatacttat caactatgtc acctttggtt ttcaaattct tccttcagcc
     8101 aatttcccag gaaaatatcg ggcgaaattc acgtggcctt caagcctttt acaagagggt
     8161 tcccttttga taaaaaataa caccggcaaa gctgtgtgga cagcgaactt tagccggaga
     8221 tctgttcggc tcgtagagcc caaaaccaat gagcaaaaca aactccaaaa taagaacctt
     8281 agaactcaag tggcggaaat ggtcatagac cagctgaatc ctgatctgat tgaagacatg
     8341 aaatacttcc ctttcatgac tttctgtatc gctaacacca cgcaagagac acggatttat
     8401 ttttgctcta aagagcttta tctgacaagc aaagacaatc acctcctcgt caaggctcgc
     8461 tcccaagaaa aacgaactcc tctcgtggag gtgaatggca agagcgtcgg caaccaaggt
     8521 attatttttt taaacaacga aagtgaaaat attggtttcc gcgcagtctc tcaatccgga
     8581 gcctacctgg aagttgagac tcgtatgaaa ccagtggact tcaaagatat aattttaagt
     8641 gaggataaaa aagaaatcat ccttaccgcg tctggtgcgg aacccgtcaa cgaggaaagc
     8701 atccaacgcc tttcagagaa cgagtggcga gttaaactgg atgccagtcg acctattctt
     8761 tacctcaaag gcgaaggcaa tattcccatg cgccaagaat tttatatcaa aggaaatatc
     8821 ccctcggagc aagcccgtcc ctcattagaa aaggattcct ttgatcatat ctacaaatcc
     8881 gagatcaacc tctccggcac ctttgcttcc ggcacttccg tcagcgcaac gactgaaaat
     8941 gaagatgtcg aaaaaaccga tttcaataaa ttccgctggc acctcacaga aattccgggt
     9001 ggagaaacct cacgtcacta tcttcgcgtc cagcacaagg aaaactcata cctagccgcc
     9061 tatgacatct atcgtgacta ctcttttgag tcggcgctct ggggcactta ttggagtcat
     9121 tcaggacttt ttgctgatat ttctttaaac tggtggatgg aaaacttcct gaccagtgaa
     9181 gccctctggg ctcggctcca ttggggaata aactttaaag agtctttgag actgacaaaa
     9241 aagacagatg aaacgagcgt tactataagc catctggaac tcctctggag agcgaatcct
     9301 ggcttccact tccaagatag aacctggggc ttgtcgcttc cctatgagat gattcaaggc
     9361 acgggaatga acatcagcag ccttggactt ggaattttct ctagcagtaa acctccgaag
     9421 aaatatcaaa gatatcttca ttggtacgac ctcaagatga attacttgat gggtgg
//
LOCUS       DATBTE010000286         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_379900, whole genome shotgun sequence.
ACCESSION   DATBTE010000286 DATBTE010000000
VERSION     DATBTE010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379900"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VIG33_10615"
     CDS             complement(<1..374)
                     /locus_tag="VIG33_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271329.1"
                     /translation="MSFDVSFSVRFCVKLFLLTGLCMGIFACQSRSKKAELPAEVDYL
                     QTKDASVVLSQGYNRSAVFSEDGEKIFYISKNRKGHKNTQIHEYDLTMQKDRRITFQD
                     GDILTLFPLENDQILYSSTTDE"
     gene            complement(371..1378)
                     /locus_tag="VIG33_10620"
     CDS             complement(371..1378)
                     /locus_tag="VIG33_10620"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY8271330.1"
                     /translation="MSNTFPSMKDHKTLLKWYHENKRDLPWRRSRDPYHIWISEVMLQ
                     QTTVAAVIPYYEKFLKLFPTVIDLAQAPEPQVLEAWAGLGYYSRARNLHRAAKIIAQS
                     GFPKTAAELLELPGFGPYTSRAVASIAYDEAVGVLDGNVIRVLSRKYGKKLEWWTSPG
                     RDQLQKISDELALRGSPAEINQALMELGATLCTPQKVQCLLCPWSSGCLARNKNLITE
                     LPLKKPRKESEIWVWRPEIHVRKSPAKGHEVALVQNHYAPFLKGQWIFPGMVLTSKEK
                     PKKFDVKHFITHHDIYIQIKKDLRAPNVLKETQWVQLKNLKQVNPSSLLTKVLKKVET
                     S"
     gene            complement(1422..>1621)
                     /locus_tag="VIG33_10625"
     CDS             complement(1422..>1621)
                     /locus_tag="VIG33_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271331.1"
                     /translation="MVSGRDISLDFKQTDEGVRVNLSLLLEINQRQVCNWTPGAPPNI
                     PPSQNCTLQQEPVYLRGQFIR"
BASE COUNT          436 a          343 c          357 g          485 t
ORIGIN      
        1 atttcatcgg tggtcgagga gtataagatc tggtcatttt ctaaagggaa cagcgtgagg
       61 atgtctccat cttgaaaagt tattctgcga tccttttgca tggtcagatc gtactcgtgg
      121 atttgagtgt ttttgtgtcc tttgcgattt ttgctgatat aaaaaatctt ttctccatct
      181 tcagagaaaa cagcggaacg gttataacct tgagaaagga cgacgctggc atccttggtt
      241 tgtaaatagt ccacttccgc tggcaattca gctttttttg atctggattg gcaggcgaaa
      301 attcccatac acagtccggt caacaaaaaa agttttacac aaaatcttac agagaaactc
      361 acatcgaaac tcaagacgtc tccacttttt ttaaaacttt cgttaataag ctcgaaggat
      421 ttacttgttt cagatttttg agttgcaccc actgagtttc ttttaaaaca tttggagcgc
      481 gtaaatcctt ttttatctga atgtagatat catgatgagt gataaagtgc ttaacatcaa
      541 attttttggg tttttctttt gaggtcagga ccattcccgg gaaaatccat tggcccttta
      601 aaaagggggc atagtgattt tggaccaaag ccacttcatg tccttttgct ggactttttc
      661 gtacatggat ttccggccgc caaacccaaa tttcactttc tttgcggggt ttttttaaag
      721 gcaattccgt aattaaattt ttattgcgag ctaaacagcc cgaactccaa ggacacaaaa
      781 ggcattggac cttctgcgga gtgcacaggg tcgctcccaa ttccatcaac gcttgattga
      841 tctcggcggg agatccgcgg agggccagtt catcggatat cttttgcaac tggtctcggc
      901 ctggagaggt ccaccattct aatttttttc cgtatttgcg agaaaggacg cgaatcacat
      961 tgccgtcaag aactcccacg gcttcgtcgt aagcaatact cgccacagct cgtgaagtat
     1021 aaggcccaaa tccaggaagt tctaaaagct ctgcggcggt ttttggaaaa cctgactgtg
     1081 caattatttt agccgcccta tgaagattgc gggcgcgcga gtaataaccc agtcctgccc
     1141 aggcttcgag gacctggggt tccggcgcct gagcaagatc gatcaccgta ggaaagagtt
     1201 ttagaaattt ctcataataa gggatgaccg cagcgactgt tgtttgctgc aacataacct
     1261 cggaaatcca aatgtgataa ggatctctgc ttcgacgcca cggcaaatcc cgtttatttt
     1321 catgatacca ttttaaaaga gttttgtgat ctttcatgga gggaaatgta ttcgacaaag
     1381 gtgctttttc caaatgctaa atggtgcttg cagagaaagt cttaacgaat gaattgtccg
     1441 cgaagataga caggttcctg ttgcaatgtg cagttttggg aaggaggaat atttggcgga
     1501 gctccaggag tccaattaca aacttgacgt tggtttatct caagtaaaag ggacaaattc
     1561 actcttacac cctcatctgt ttgcttaaag tctaaagaaa tatctcgtcc actcaccatt
     1621 a
//
LOCUS       DATBTE010000287        14580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1130409, whole genome shotgun sequence.
ACCESSION   DATBTE010000287 DATBTE010000000
VERSION     DATBTE010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 14580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14580
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130409"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..338
                     /gene="rpsU"
                     /locus_tag="VIG33_10630"
     CDS             135..338
                     /gene="rpsU"
                     /locus_tag="VIG33_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEY8271332.1"
                     /translation="MSMTKIKDGESFESAFRRFKKSCEKAGILSEVKKREHFEKPSVR
                     LKKKSIAARKRATKKSKKGWSND"
     gene            357..803
                     /locus_tag="VIG33_10635"
     CDS             357..803
                     /locus_tag="VIG33_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEY8271333.1"
                     /translation="MEIREQLMADVKDAMRAKDQLKLNTLRFLQAAIKNREIELRPNP
                     ISSDEVMGVLKRLVKQRKESIEQYQAGNRQDLVDQEAAELKVLEAYLPAQMGKEQVEK
                     VVTEVIAALGAKSVKDMGPVMKEVIARTAGTADNKTVSEVIKSKLT"
     gene            825..2681
                     /gene="dnaG"
                     /locus_tag="VIG33_10640"
     CDS             825..2681
                     /gene="dnaG"
                     /locus_tag="VIG33_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY8271334.1"
                     /translation="MKFSPEFIEKVRDSSNLVDIISQYTQLKPTGSGLMGRCPFPDHQ
                     EKTPSFSVSEIKQVYHCFGCHKKGNIFTFLQNYQGMNFPEAVEFLANRAGIAMPERSS
                     ETEERYDRQKERRKLLGRVNQLASQYFVEQLKRTPPEHPVKKYISKRGLSQEVLDVFH
                     IGYSTAEWDGLTKYLESKKVPMELVEEARLAKARTGGKSGHFDIFRDRLMFPILNPLG
                     EAVAFGGRIIEQGEPKYLNSPESPVFVKGKILYGLAQTARYIRTEDQAIIVEGYMDLV
                     SLYQADIRNAVATMGTALTPDHGKLLKRMTKNIVVLFDGDSAGQEAAERSLPTLLSAD
                     VHPKGLTLPDGMDPDDFVKANGAEALKDLMAKAPDLFVMILQRWMEDYRGEASQKVKI
                     CDQIKPVLDVIPDSRLKDLYLQEAARRMSVPTIWLKEALFKPTNKLVVEAENIRRERA
                     AYSKPRHEHPKIESDEPKTVPNAPRFLLKGAPKPEILLMGLVLKSRANFEIYLQENIC
                     ENISYQAIQEILKKAADVYRQDPNKFDKLTSLLVSFVDHPEAFFVNENANESFDDENE
                     RKLLRDVFKRVRDNSLRAQAKKLARDLKTEPSAEKMEQIMNIQRNRHSLNKN"
     gene            2751..4577
                     /gene="rpoD"
                     /locus_tag="VIG33_10645"
     CDS             2751..4577
                     /gene="rpoD"
                     /locus_tag="VIG33_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY8271335.1"
                     /translation="MKSNAPNASSKEPSKVLTVLEQEALIKEEIQKFIKLFKEKGNLT
                     IEEINELLPPEIVAPSVLDAFMHGLEAAGAVISDLIETEKEEGDGSFLADPDKADEEA
                     DEDEIAESEDVKGNDPVRLYLRKMGSVSLLTREGEVEIARRIEKGEREIVKAILLSPL
                     GTYEIIQLGKRLDEGRIKVKAIFRGLEDEETQYDEKEYIDKIHELIGTVTAYHESVKK
                     YFDVLRKEETNTPARKAAMDELLKVNDKLMASFESINFNRKTINRIVIKFKNLVGRML
                     ILRRRIKDGIEKTFSKDVNSLVERLKLVDGNEKELTKMTRETGLNYNKFRSYAIQAQE
                     ASVRLTRLHSETEMNFRWIQDTYTAIWKGEREADLAKSELVEANLRLVVSIAKKYTNR
                     GLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRAIADQARTIRIPVH
                     MIETINKLVRTSRYLVQELGREPIPEEIAEKMDMPVDKVRKVLKIAKEPISLETPVGE
                     EEDSHLGDFIEDKKVISPAEAIVNLNLAEQTRRVLSTLTPREEKVLRMRFGIGEESDH
                     TLEEVGQDFNVTRERIRQIEAKALRKLRHPSRSNKLKAFIDG"
     gene            4775..6313
                     /locus_tag="VIG33_10650"
     CDS             4775..6313
                     /locus_tag="VIG33_10650"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEY8271336.1"
                     /translation="MNLEKVPFEHLLQQSFLLPSFRQGQREIISAILEKKDALAVLPT
                     GGGKSLCYQFPAVYTQQLVVVISPLIALMKDQVASLQRQGIPAGCLYSGQADSEKRAI
                     FMEIAKGGAYLLYLSPERVQKEGFQKWIQGRSIALFAVDEAHCVSQWGHDFREEYGQL
                     KVLKSLRPEVPILALTASATPMVLNDVAKNLGLVKPERMVHGFYRPNLYYQVEHCSDE
                     DAKFLILNQAIRQTSEGRVIIYCGTRRVTEELSATLSRIFDKVGYYHAGLSTEERTAN
                     QEAYTRGDLRILVATNAFGMGIDQPDVRLVVHFQMPGNIDSLYQEMGRAGRDGKESTC
                     LMLYAKKDKGLQSFFIDNSEAPKAIKSARWRNLEALVNYSEGGECRHAEILTYYKDSK
                     RIESCGHCDSCDPRSPRRIMKPVKPVVTKLVKAKSRRSKAVGEDIELDTSQELRFEAL
                     RKWRKQKAQELDVPAFVVFSDQTLRQLAIKNPQSLAELENIYGIGESKLEKFGWDLLA
                     ELGQ"
     gene            complement(6346..7095)
                     /locus_tag="VIG33_10655"
     CDS             complement(6346..7095)
                     /locus_tag="VIG33_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8271337.1"
                     /translation="MQKNWVLIRGLVRGVGHWSDFPERIKSAFPNDNFEFLEIPGNGV
                     LYKEKSPVSMEKMVEALRQESQFLRQGKKIHILAISLGAMLATAWAARYPAEVAELIL
                     INSSSAKHSKFYERLRPENYLKIAGLFLGFKTRYTVEEREQEILKMTANSSERRDFAL
                     PLWTEESTAHPIEIENFFRQLFAANSYTFPTKAPVPTTILTCENDHFVNVSCSKNLAK
                     SWDCAIHIHPWAGHDLPLDDPDWVLKHLASI"
     gene            7300..7376
                     /locus_tag="VIG33_10660"
     tRNA            7300..7376
                     /locus_tag="VIG33_10660"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7334..7336,aa:Ile,seq:cat)
     gene            complement(9097..9495)
                     /locus_tag="VIG33_10665"
     CDS             complement(9097..9495)
                     /locus_tag="VIG33_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY8271338.1"
                     /translation="MKLQPKIAIYIRTAIHGKDSRQTLKAQEAACRKFIDSQLPELAS
                     VKGTISVYQDLGHSAMDMKRPGLKKLLVDLKNMQMRTIITTDMIRLFRRPQDAATLQR
                     TLSELNVTALAVDGSILGSNPPVTERMAVL"
     gene            complement(9479..9679)
                     /locus_tag="VIG33_10670"
     CDS             complement(9479..9679)
                     /locus_tag="VIG33_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271339.1"
                     /translation="MSSKPTYEPALELDEEDMKVELVAVTDESKNAEARTERVEMLIA
                     KVILLGQQRGRPRKDTSDEIAA"
     gene            complement(9676..9930)
                     /locus_tag="VIG33_10675"
     CDS             complement(9676..9930)
                     /locus_tag="VIG33_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271340.1"
                     /translation="MSPAKFKQIREHLGLTQPELAELFGLSGKGPISHFEIGFRTPSP
                     LIAALMSYLESLSERKAQEFLVEFKRHMEKVQKSAKSSSK"
     gene            complement(9927..10358)
                     /locus_tag="VIG33_10680"
     CDS             complement(9927..10358)
                     /locus_tag="VIG33_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271341.1"
                     /translation="MNTEEQENKNKDVIEKDGLMKKEFRLVISQEAEAAVTDITNAVN
                     DGFEAGRATRFDVANSMLLWFKAHAPEDVIFELRRKFADAVTMLEAIQKKAKSSGELP
                     PEIKAALEKYYFGVTTSGIKKAKNNLKQECITDIHKDSEVA"
     gene            complement(10534..11346)
                     /locus_tag="VIG33_10685"
     CDS             complement(10534..11346)
                     /locus_tag="VIG33_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271342.1"
                     /translation="MTKNYLQSRDRALEFTARFGFITRDLFFEYICPLKRALKYYHWA
                     ALQEEGLIYVSRTLSDVFYLTRKGRLFYSKPSAPARSIYFIPHDKIVGQILLSLKASG
                     FVENYWTERDLKTDRLIACSILGTGQLLKYPDLVIDMKGANRSLRVALEVERSQKAAF
                     RYDQISMAYISANRIGLLVFACSTKGTVSLIERAFQGELFQKAAKVPATFLLNDFSKS
                     GIDANATFMGRQLKLRRLILAALQRPESDWQNNMDKTWKVVHGLSMKSKGIA"
     gene            complement(11343..11639)
                     /locus_tag="VIG33_10690"
     CDS             complement(11343..11639)
                     /locus_tag="VIG33_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271343.1"
                     /translation="MKQGLILVISWLMGACAHTPRCGNPDGAKIIFVEGPTCKVRIRQ
                     VTVGSEMKVPESLKNTNFSSFQIEWIEPSVNEGRVSLGHFELVPSDSRTSGVDK"
     gene            complement(11636..13330)
                     /locus_tag="VIG33_10695"
     CDS             complement(11636..13330)
                     /locus_tag="VIG33_10695"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEY8271344.1"
                     /translation="MSDKNQKNNNMSDQGFLFLAACLFVGVGWSKFKMPMLMFYYKWR
                     FLVALLITLAIYALFLKLKKMFLKWTAAGSIEKDVLAYNPDEDSVYAGMTEKGKNVYI
                     KLAFRRMHVQIVGTTNAGKTESVIIPWTIDDIKKGRGCIIIDGKSDRSLLDKIYAYAK
                     KHGRLQDVKILSLCNVDISSTFNPLEGGSSLEIAERVFNALNFENEYFKSIQYDAFLH
                     SLLLLEAVKIKATPYRVLELLRSDSQMSHLIKSVTDPELCLWVAEFCKLSKSEREQRT
                     SGLVSQLQSFVVGETGLIFNSERSEINLERALENGEIIYCQLPALKIPTLGKATGKMI
                     LQCLQSAVASRHLGAAKSQKFFSVYLDDFTEYLTPSFVTLLNKSRSANVGIVFAHQAL
                     GDLSALGDGVKNSILTNSNLKIFMRTNEPESAEYFSSVIGTSLTSKVTERQKSGFIGS
                     TKTGDGSVRDAEEFKFHPNVFKQELGVGEAVVVLPHGKGSLPVRMKFRKSFDLDIPVI
                     PTLFKSNPVGLPPIPTVEEIKQGLDPALNLANPSKTSEESTAESILKASTLTKEAS"
     gene            complement(13320..14279)
                     /locus_tag="VIG33_10700"
     CDS             complement(13320..14279)
                     /locus_tag="VIG33_10700"
                     /inference="COORDINATES: protein motif:HMM:NF015688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HEY8271345.1"
                     /translation="MAIMNKLSVIKDRVCEFFLKLKSKFFKSSDISGKTEVDYRSFKI
                     LGMTLLFIFIIVIFLLPDDPPVEFTEKVAHVSSESGKSEGNASQKSGGGAAQNLWSSS
                     RISMPGGANSQANYNASMILGNQNGNAKSQLRAGVRMPLRILDKFIVSQESVPILAEL
                     IFDSVTDSGLKLPAGTRFYGEASFARGSDRATIQFRQISVPSGQIRGISGTALGKDGQ
                     PGVPGHVFSDGMKNTAGQVITSFVGGLAAGSIETDVFGRSKGGIENGLLSAVAATAKD
                     RANNYGEKLKTEREWIEVLAGAECDALLNESINLQNGGDNYER"
     gene            complement(14270..>14580)
                     /locus_tag="VIG33_10705"
     CDS             complement(14270..>14580)
                     /locus_tag="VIG33_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271346.1"
                     /translation="LILKVQRVGISQNDFVLIDAKITYSGFETQQVKPDQFWVTQNGV
                     SRVINGLFISDTKVLKNKPLLVGLSLSKSDLESKKPVSIELRGNNKIKVQIPEATVWQ
                     "
BASE COUNT         4054 a         3174 c         3281 g         4071 t
ORIGIN      
        1 cagggactta tggtttgccc tattccagag gctttgttat agactgactc tttgaattcc
       61 ccaggggaac tagctacagg attccttttt tgagaatcct acttccgaca cacgtgagag
      121 gcaggtgatt ttcagtgtca atgacgaaaa tcaaagacgg agaaagtttt gaatcagcat
      181 tcagaagatt caaaaagtct tgcgaaaaag caggcatcct ttctgaagtt aaaaaacgtg
      241 agcatttcga gaagccaagc gttcgtttga aaaagaaatc gatcgcagct cgtaagcgtg
      301 caacgaagaa atccaaaaaa ggctggagta atgactaagc ccttaggagg tttctaatgg
      361 aaataagaga acaactcatg gcggacgtaa aagacgccat gagagcgaaa gatcaattaa
      421 aactcaacac tttgcggttc cttcaagcag cgattaaaaa tcgtgaaatt gaacttcgcc
      481 ccaacccgat cagctcagac gaagttatgg gcgtgctcaa aaggttggtt aagcaaagaa
      541 aagaatcgat tgaacaatat caagcaggca accgtcagga tctcgtcgat caagaggctg
      601 ctgaattgaa agtgcttgag gcttatttgc ccgcacaaat gggtaaagaa caggtagaga
      661 aagttgtcac ggaagtgatt gcggctttgg gtgcgaaatc tgtgaaagac atgggtcctg
      721 taatgaagga agtcattgct cgcacagccg gtaccgccga caacaagaca gtgagcgaag
      781 ttattaagtc taaacttact taaacgttgt gatttcggag tcgcatgaaa ttttctccag
      841 agtttattga gaaagtcagg gattccagca atctcgtcga tattatctct cagtacacac
      901 agctaaaacc tacaggaagc ggccttatgg gccgctgtcc ttttccggat catcaggaaa
      961 agacaccgag cttttccgta tccgaaatca agcaagtcta tcactgcttt ggctgccata
     1021 aaaagggcaa tattttcact ttccttcaaa actatcaggg aatgaatttt cctgaggctg
     1081 tcgaatttct tgctaaccgt gcagggattg cgatgcctga gagaagttca gagactgagg
     1141 aacgttacga tcgccaaaaa gaacgcagaa aacttttagg acgggttaat cagctcgcga
     1201 gccaatattt cgttgagcaa ttgaagcgaa cgcctcctga gcatcccgtt aaaaaatata
     1261 tttccaaacg aggtctgtcc caagaagtct tggatgtttt ccatataggc tattcgacag
     1321 cggagtggga tggcttaacg aagtatttgg aatccaaaaa agtgcccatg gagcttgttg
     1381 aagaagctcg acttgccaag gcccgtaccg ggggcaaaag tggacatttt gatattttcc
     1441 gcgaccgcct gatgtttccg atcttgaacc ccctcgggga ggccgtggct ttcggtggac
     1501 gtatcattga acagggcgag ccgaaatacc tcaatagtcc tgagagccca gtctttgtta
     1561 aaggaaaaat tttatacggg cttgcgcaaa cagcccgata tatacgaacc gaagatcaag
     1621 ccatcattgt cgaaggatat atggacttgg tttctttgta tcaagcggat attcgaaatg
     1681 cagtggcaac catgggaaca gctttaactc cagaccatgg aaaactttta aaacgtatga
     1741 caaaaaatat cgttgtactt tttgatggag attccgcggg ccaagaggcc gctgagcgca
     1801 gcttaccgac cctcctttct gccgacgttc atccaaaagg cttaactttg ccagatggga
     1861 tggatcctga tgatttcgta aaagcgaatg gagcggaggc attgaaggat ttgatggcga
     1921 aggctccgga tctttttgtg atgattctcc agcgttggat ggaggactac agaggagaag
     1981 cttcccagaa agtaaaaatc tgtgatcaaa tcaagccagt actggacgtg attcctgatt
     2041 cgcgattaaa ggatctttat ttgcaagaag cggctcgtcg catgagtgtg ccaacaatct
     2101 ggctcaagga agctttgttt aaaccgacaa ataaacttgt cgtggaagcg gaaaatattc
     2161 gccgagagcg cgcagcttac tcaaaaccga ggcatgaaca tcccaagatt gagagtgatg
     2221 aaccaaaaac tgtgccaaat gctccccgat ttctcttgaa aggagcacca aaaccagaaa
     2281 ttttactgat gggtttggta ctcaaaagtc gtgccaactt tgagatatat ctgcaagaaa
     2341 acatatgtga aaatatctct tatcaggcga ttcaagagat cctaaagaaa gctgcggatg
     2401 tctatagaca agatcctaat aaatttgata aattaaccag tcttctcgta tcatttgtag
     2461 atcatcctga agcgtttttc gtaaatgaaa atgcgaacga gtcatttgat gatgagaacg
     2521 aaagaaagct tctgcgcgat gttttcaaaa gagttcgtga caacagtttg cgagctcagg
     2581 cgaaaaagct agctcgagat ctgaaaacag agccgagcgc tgagaagatg gagcagatta
     2641 tgaatattca gagaaacaga cactctttga ataaaaactg aatctgaatt ctgaattgta
     2701 aattaaagtc ataagaaaat tttttacgcg cacaaaaaaa tggggtctaa atgaaatcaa
     2761 acgcacccaa cgccagctct aaagaaccat caaaagttct cactgtccta gagcaagagg
     2821 ctttgattaa agaagaaatt cagaaattca tcaagctctt taaggaaaaa ggcaacctga
     2881 caatcgaaga gatcaatgaa cttcttccgc cagagattgt ggctccttca gtcctagatg
     2941 ccttcatgca tggtcttgaa gccgctggcg cagtcatctc ggatttgatt gaaactgaaa
     3001 aagaagaagg cgacggctct ttccttgccg atccggacaa agccgacgaa gaagctgatg
     3061 aagacgaaat cgctgaaagt gaagacgtca aagggaatga tcccgtgcgc ctatatcttc
     3121 gcaagatggg aagtgtgtca cttcttactc gtgaaggcga ggtagagatt gcccgccgta
     3181 tcgagaaggg ggagcgcgag atcgttaagg cgattctttt gtcacctctc ggtacctacg
     3241 aaatcatcca actgggtaaa cgtttggatg aaggtcgtat taaagtaaaa gccatcttcc
     3301 gcggcttgga agatgaggaa actcaatacg acgaaaaaga atacatcgac aaaatccatg
     3361 aactcattgg cacagtgacc gcttaccatg agtcggtgaa aaaatatttt gatgttcttc
     3421 gtaaagaaga aaccaacact cctgcgcgta aagctgcgat ggatgagttg ttgaaagtaa
     3481 atgataagtt gatggcaagc tttgaatcta tcaacttcaa tcgtaaaacg atcaaccgga
     3541 tcgtgattaa gtttaaaaac ttagtgggcc gtatgttgat tctccgccgt cgtatcaaag
     3601 atggtatcga gaaaactttc tctaaagacg tgaactccct ggttgaacgt ttgaagttag
     3661 ttgatggtaa cgaaaaagag cttacaaaaa tgactcgtga gactggtttg aattataaca
     3721 agttccgttc ttatgcgatc caagctcaag aggcatcagt tcgtttgact cgattgcata
     3781 gtgaaactga gatgaatttc cgttggattc aagacaccta caccgctatt tggaaaggtg
     3841 agcgcgaagc ggatttggcg aaatctgaat tggtagaagc caacctgcgt ctcgtggtgt
     3901 ccatcgctaa gaaatacacc aatcgcggac tccaattctt ggatttgatc caagaaggga
     3961 atattggttt gatgaaggcc gtggataaat ttgagtaccg tcgtggttac aaattctcga
     4021 cttatgcgac ttggtggatc cgtcaagcca tcacccgcgc gattgccgat caggcgcgta
     4081 cgattcgtat cccggttcat atgatcgaaa caatcaataa gctggtacgg acctctcgtt
     4141 atctcgttca agagcttggc cgtgagccta tccctgaaga gattgcggaa aaaatggata
     4201 tgccagtgga taaggttcgc aaggtactga aaattgcgaa agagcctatt tctctcgaga
     4261 ctccagtggg tgaggaagaa gattctcact tgggagattt catcgaagat aaaaaagtca
     4321 tcagtccagc ggaagccatt gtgaatttga acttggctga gcaaacccgt cgcgtccttt
     4381 ctactttgac tccaagagaa gaaaaagtcc tgcgtatgcg tttcggtatc ggtgaagagt
     4441 cggatcatac tttagaagaa gtaggtcagg attttaacgt gactcgtgag cgtatccgcc
     4501 agatcgaggc caaggcattg agaaagcttc gtcatccaag ccgcagcaat aaactgaagg
     4561 ctttcattga cgggtaggct gtagcctgga cttagtttgg tttattttaa aacccgcatt
     4621 ttaaaaggtg cgggtttttt attttcagag gtgttcaaat gaaaatccgg gtttcgtttt
     4681 tgctgtccgg ttttaagttt gaggtctcaa gattctcact gtaagcacgg cattgttatt
     4741 gctctgttgg tggctttcaa cgaaagtgat ctgtatgaat ttggaaaaag tgccctttga
     4801 acatcttttg cagcaatcat ttcttttgcc ttcgtttcga caagggcagc gagagattat
     4861 ttccgccatt ctcgaaaaga aagatgcctt ggcggtttta cctacgggcg gcggtaagtc
     4921 tctatgttac caatttccgg ccgtttatac tcagcagttg gtggtggtga tttctccttt
     4981 gattgctttg atgaaggatc aagtagcttc tctgcaaagg cagggcattc ccgcagggtg
     5041 cttgtattca ggacaggctg attctgaaaa acgtgctatt tttatggaga ttgctaaggg
     5101 tggggcttat cttttgtatc tttctcctga gcgggtgcaa aaagaaggtt ttcagaaatg
     5161 gatacaggga cgctccatcg cgctctttgc agtggatgag gctcactgtg tgtctcagtg
     5221 gggacacgac tttcgtgaag agtatggaca gttgaaagta cttaaaagcc tgcggcctga
     5281 ggttccgatt ttagccctga cggcatcggc aactccaatg gtattaaatg atgtggctaa
     5341 aaatctagga ctcgtaaagc ccgagcgtat ggtccatgga ttttatcgtc ccaatcttta
     5401 ttatcaagtt gaacattgta gcgatgaaga cgcgaaattc ttgatactaa atcaggccat
     5461 tcgccaaact tctgaggggc gcgtgattat ttattgcggg actcgtcggg tgaccgaaga
     5521 gctttctgcc acgctttcgc gaattttcga taaggtgggt tactaccacg ccggactttc
     5581 cacagaggaa agaaccgcga atcaagaggc ctatacacgg ggggatttac gcattttggt
     5641 tgccaccaat gcttttggga tggggattga tcaaccggat gttcgcctgg tggtgcactt
     5701 tcagatgccg ggaaatatag attctcttta tcaagagatg ggaagagcag gccgcgatgg
     5761 caaagagtct acttgcttga tgctctatgc aaagaaagac aaggggcttc aatccttttt
     5821 tatcgataac tcagaggcac caaaggccat aaaaagcgcg cgttggagaa atctcgaggc
     5881 cttagtcaac tactctgaag gtggagagtg tcgtcatgcg gagattttga cttattataa
     5941 agattcaaaa cggatcgaga gttgcggaca ttgcgatagt tgtgatccca ggtccccgcg
     6001 ccgaattatg aaacctgtga aaccggtagt gactaagctg gtgaaggcta aatcccgtcg
     6061 ctcaaaagct gtcggcgaag acattgagtt ggatacttcc caagaactgc gttttgaggc
     6121 gcttcgtaaa tggagaaaac aaaaggccca ggaattggat gttccggctt tcgtggtttt
     6181 tagtgatcag acactcagac agttggccat taaaaatccg caaagtcttg cggagctgga
     6241 aaatatttac gggattggcg agtccaagct tgaaaagttc ggttgggatt tacttgcaga
     6301 attaggtcag taaatttttt ttagaatgca tttcaagaat gttttctaga ttgaggcgag
     6361 gtgttttaag acccaatctg gatcgtccaa tggcaaatcg tgaccggccc aggggtgaat
     6421 atggatagcg cagtcccagc ttttggcaag atttttagag caagagacat tgacaaaatg
     6481 atcgttttcg caagttaaaa tggtggtcgg taccggggcc tttgtgggaa aggtgtaaga
     6541 attggccgca aatagctggc gaaaaaagtt ttcgatttca atagggtgag cggtcgactc
     6601 ttcagtccaa aggggaagtg caaaatccct gcgctcggaa gagtttgcgg tcattttaag
     6661 aatttcctgc tccctttcct ctacagtgta gcgagtttta aaacctaaaa agagtccagc
     6721 aatttttaaa tagttttcag gtctgagacg ctcgtaaaac tttgaatgtt tcgcagagct
     6781 agagttaatc aaaatcagtt ccgcaacttc tgcaggatag cgagcagccc aggctgtggc
     6841 gagcatggca cccaatgaaa tggcaaggat atggatcttt ttaccttgtc gaaggaattg
     6901 gctttcttgg cggagagcct ccaccatttt ttccatgcta acaggacttt tttctttata
     6961 gagaacgccg ttgccgggaa tctctaagaa ttcaaagttg tcgtttggaa aggcgctttt
     7021 aatccgctct ggaaaatcac tccagtgccc caccccccga accaatcctc gtataagcac
     7081 ccaatttttt tgcatgagtt atttttaaat agataagatc gtgagtcaaa aaaaaagccc
     7141 gctggagaat ttcaagaaat ggtcctgaaa gtaagctagg tgcataatta aactaaagaa
     7201 ttcccgatag gcaaacagat cagatcactt tgtgttgcat aacggaagca gttgctaaat
     7261 atctgatttt tggtaagttt aggctcttcc gaaaattgag gggggctagc ttagttggtt
     7321 aaagcagccg gctcataacc ggcggatcgg gggttcgagt ccctcgcccc ctaccaattt
     7381 cttcaacctg caatttttcg atgcccatat tatgaaaatc agattcagcg actctcccct
     7441 caggggggag ctgccggtgt ttgcttaaat caatgacgtt ctctttattg tccgctgacg
     7501 cagataaact ctccgctatg aagttttgat taagatcatg cttaagtctg aattgaattt
     7561 taagaccatc atggcttacc aggacagaat gaatgacctc tttaagaagg gtctttttca
     7621 ttatagcgct ggcctttgcc caacctcttt ggaaagcttg gatatgggtc ctaagatcta
     7681 aaatcgcatc atcaacgtca ctttgctgaa attctttggc ctttaatgtt tcaagctctg
     7741 actctagatt tcttttttta cggctaaggt cttccagttc tttggctacc gccctgatcg
     7801 cctcagagct agcatcaagt tcggcttgga ttttaaaggt gtttctgacg gcaaggctta
     7861 ttttttgaag ctctatcgtc agacgggcct tttctgtttt taaggcttca ggagagtcgg
     7921 tcacagattt tctaatgcgt tcttcgattt tgtcgaaata cccagccgtt aaaagaattt
     7981 cggccacata ttcaccgatc tttgcctcaa tctgatcagc cttaatccgt ttaattttac
     8041 acgtaacgtc atcgcctttt ttagaactat gaacatagta gcggtgaact ttgtttcgac
     8101 catgggctga ttgaccgaca agtttgcgac cacattccac acaaatgcaa agtcctgagg
     8161 ctgcaaaaac tcgcgccgtc gacgtatcca gtcttgaacg ctcctgcttt cggttttcat
     8221 caaggacgta ttgaacttga tcgaaggtct cttgatcaat aagagcaggc catgaggcct
     8281 tggcaatacc gtagcgttgc caagccttca actttccctg atctttattt ctattcactt
     8341 tgttaacttc gcggagtccc acgtaagctt tattttgaag aacaaagttc agagaatcaa
     8401 tcgtccacct tccagcttgc gcaagacggt ttcttttatc actttttgcc ttgggcttga
     8461 tacctatatt ctctagagct actattgtcc ggctcaaggt tctattttcc aaaaacaaat
     8521 taaagatacg gcgaacatca tcggcttctg cttcattgac gataaaggta gttttattcg
     8581 acggatcttt atcataaccc aaaatggcag ggccgccgtt tagaagtcct ctctgggctc
     8641 gggagtggca attaatcgcg acacgttcgg agatctgctc ccgttcgaat tgagctaaat
     8701 ttatcatatt atagagcatc atttttcctg aaggcgtcga ggaatcgaat tgttctttaa
     8761 tcgacaaaaa catggctccc tgactctcaa gagttttatg aaagtcgcaa aaatcaggga
     8821 tgttgcgaga aagacgggaa agctccgtca ccatcactgc gttgatgtgg ccgtgacgaa
     8881 gtgccttcat cattcgttga taggcaggac gattggtgtc ctttgctgag aagccatcgt
     8941 caacgaagac ctcaacaagt tgcccccaac ctggatcttg catattctta atgtcgataa
     9001 aagactgcat acggtgacgt tgattatcaa gcgatccttc aataacattt gcctgctcgt
     9061 cagtggaaac tcttgaataa attccgatat ggatagtcat aacaccgcca tcctctctgt
     9121 cactggagga ttacttccaa ggatgctgcc atcaacagcc aatgccgtga cattcagttc
     9181 tgaaagagtc cgctgaaggg ttgccgcatc ctgcgggcgg cgaaacaagc ggatcatatc
     9241 tgttgtgatg atcgttcgca tttgcatatt ttttaaatcc actaaaagct ttttaagacc
     9301 agggcgcttc atatccatcg ccgagtgtcc aagatcttga tagacggaga tggttccttt
     9361 tactgacgca agttcaggaa gctgactgtc gataaatttt cggcacgcgg cctcttgtgc
     9421 tttgagagtc tgacgagaat ccttaccatg aatggcagtt cgaatgtaaa tggcaatctt
     9481 aggctgcaat ttcatcggac gtatcctttc tcggtcggcc ccgttgttgg cctagtaaaa
     9541 tcacctttgc aatgagcatt tcaactctct cagttcttgc ttctgcattt tttgattcat
     9601 cagtaacggc aaccaattcc actttcatat cctcttcatc aagctctaaa gccggttcat
     9661 acgtgggctt actactcact tagatgaact cttagcggac ttttgcacct tttccatatg
     9721 ccgcttaaat tctactaaaa actcctgagc ctttcgctct gagagacttt ctagataact
     9781 cattaaggcc gcaatcaaag gacttggtgt cctaaatcct atttcaaagt gactgatggg
     9841 accttttcct gagagtccaa agagctctgc gagttccggt tgcgtaagtc ccaggtgttc
     9901 tctaatttgt ttaaactttg ccgggctcat gcaacctcgc tgtctttatg tatatctgtg
     9961 atacactctt gtttcaagtt atttttagct ttctttatac cactcgtcgt cactccgaag
    10021 tagtacttct caagggcagc cttgatctca ggcggcaact ctccggaaga cttggccttt
    10081 ttctgaatgg cctctagcat cgtaaccgcg tcagcaaact tccgacgcag ctcaaaaatg
    10141 acgtcctctg gagcatgcgc tttgaaccat aagagcatag aattggcgac gtcaaagcgg
    10201 gtcgcacgcc cggcttcaaa gccatcattt acggcattgg taatatctgt gactgccgct
    10261 tccgcctcct gagagataac aagtctaaac tcttttttca tcaaaccatc tttttctatg
    10321 acgtctttat ttttattttc ttgttcttct gtattcatat ccccacctcc tcatttatat
    10381 tgtttttaac tctaaacctt cttccaccca accccggccc ccggttcgtg accaccttca
    10441 accaactcgt tgggtggggg tggtcacgaa cagctactgc tgagggggcc ggggttgggt
    10501 ggccactatc tagtggattt tccctcttat ttttcatgcg atgcccttgg atttcatgga
    10561 taatccatgg acgactttcc atgttttatc catgttattt tgccagtctg attctggccg
    10621 ctggagagcg gctaaaatta atcttctcag ttttaattgt cgtcccatga acgttgcatt
    10681 tgcgtctatg ccgctttttg aaaaatcatt gaggagaaaa gtcgctggaa ccttggcggc
    10741 tttttgaaat agttcccctt gaaaagctcg ctcaattaaa ctcaccgtgc ctttggtaga
    10801 acaggcaaag actaaaagtc caattcgatt ggcactaata taagccattg aaatttgatc
    10861 atagcgaaag gcggcctttt gtgatctttc aacttctaag gcaacccgga gagatctatt
    10921 tgcccccttc atatcaataa caagatctgg atatttcaaa agctgtcccg tccctaatat
    10981 tgaacaagcg ataagtcggt ctgtttttag atccctttca gtccagtaat tttcaacaaa
    11041 gcccgatgct tttaaagaca ggagaatctg ccccacgatt ttatcatgtg gaataaagta
    11101 aatggatcgc gccggagctg agggtttcga ataaaaaagt cgccctttcc gggtcagata
    11161 gaaaacatca ctgagagtcc ttgaaacgta aatgagtcct tcttcttgaa gagctgccca
    11221 atgataatat ttaagagccc gtttcagtgg gcaaatatat tcaaaaaata gatccctcgt
    11281 gataaagcca aatcttgcag tgaactcaag agctcgatct ctagattgca aatagttctt
    11341 tgtcatttat ccactccgga ggttcgagaa tccgaaggca ccagttcaaa gtgaccaagc
    11401 gagactctcc cctcattgac agatggctca atccattcaa tctgaaagct agagaagtta
    11461 gtattcttaa gactctctgg cactttcatt tccgatccca ctgtgacttg gcgtattctg
    11521 actttacagg tgggtccttc aacgaaaatg atttttgctc catcaggatt cccacatcga
    11581 ggcgtatgtg cacaagcccc catcaaccac gagataacca agataagccc ctgcttcatg
    11641 aagcctcctt cgttaaagtt gaagctttta aaattgactc agccgtcgat tcttcagaag
    11701 tcttggacgg gttcgcaaga ttcaatgctg gatcaagacc ttgcttaatt tcctccactg
    11761 ttggaatagg aggaagtccc acagggtttg acttaaaaag agtgggaatc accggaatat
    11821 ccaaatcgaa ggattttcga aatttcattc ttaccggaag acttcctttg ccatgcggta
    11881 acaccacgac ggcttcaccg acgccaagct cttgcttaaa aacattgggg tgaaatttaa
    11941 actcctcagc atcacgaaca gatccatcac ctgtctttgt agagccgata aatccgcttt
    12001 tttgtctttc agtgaccttg gaagtgagag aagttccgat aacacttgaa aaatattcag
    12061 cagactctgg ctcattggtc cgcataaaaa tcttcaggtt tgagttcgtc aggattgaat
    12121 tcttaacccc atcacctaga gctgacagat ctcccagcgc ctggtgggca aaaacgattc
    12181 ccacgtttgc acttcgtgat ttattaagaa gggtgacaaa gcttggggtc agatattccg
    12241 taaagtcatc caaatagacc gagaaaaatt tctggctttt cgcggccccg agatgacggg
    12301 aagctacggc actttgcaga cattggagaa tcatcttccc cgttgcctta cctaaggttg
    12361 gaattttaag agccgggagc tggcaataga tgatctcacc gttttcaagt gctcgttcca
    12421 ggtttatttc agagcgctct gaattaaaaa tcaaacctgt ttcgccgaca acaaaacttt
    12481 ggagctgaga gacaaggcca ctggtgcgtt gctcacgttc agatttactc agtttacaga
    12541 attcagccac ccataagcaa agctcgggat cagtgacact ttttataaga tgagacatct
    12601 gggagtcact tcgcagaagt tctaaaaccc ggtacggtgt cgccttgatt tttacggcct
    12661 ctaacagaag aaggctgtgt aaaaacgcat cgtactggat actcttaaaa tactcatttt
    12721 caaaattgag ggcattaaaa actcgttccg caatctcaag agaagaaccg ccctcgagag
    12781 gattaaaggt tgagcttata tccacattac aaagcgaaag gatctttacg tcctggagac
    12841 gtccatgctt ttttgcgtag gcataaattt tatccagaag actgcgatct gattttccat
    12901 caatgataat gcatcctcgt ccttttttaa tgtcatcaat cgtccaagga ataatgacag
    12961 attctgtctt tccagcattg gtagtgccaa cgatttgcac atgcattcta cggaaggcga
    13021 gcttgatata gacattcttt cccttttcag tcattcccgc atacacggaa tcttcgtcgg
    13081 gattataagc caaaacgtct ttttcaatac ttcccgcagc ggtccatttt agaaacatct
    13141 tttttagctt caaaaaaagc gcataaattg caagagtaat caggagagcc accagaaagc
    13201 gccacttata ataaaacatt agcataggca ttttaaattt agaccaaccg acaccaacaa
    13261 aaagacaggc tgccaaaaat agaaagcctt gatcggacat attgttattt ttttgatttt
    13321 tatcgctcat aattgtcacc tccgttttgt aagttaattg attcattgag aagggcatcg
    13381 cactcagctc cggctaagac ttcgatccat tcacgttctg ttttaagttt ctcgccatag
    13441 ttattggcgc gatccttggc ggtggccgcg accgccgaca atagaccatt ttcaattccg
    13501 cccttggagc gaccaaagac gtcagtttca atacttccag cggcaagtcc acctacaaag
    13561 cttgtaataa cctggcctgc ggtatttttc attccgtctg aaaagacgtg ccctggaact
    13621 cccggctgtc cgtcttttcc aagcgccgtc ccggagattc ctcggatttg accactagga
    13681 actgagattt gccgaaactg aatggtggcc cgatcacttc cacgcgcaaa gcttgcttct
    13741 ccataaaatc tagttcccgc cggaagtttg agtcctgagt ccgtgactga gtcaaaaatt
    13801 aactctgcta aaataggcac tgattcctgg gagacaatga atttatcaag gattcttagc
    13861 ggcattctaa cccctgctcg aagttggctt ttggcgttgc cgttttgatt acctaaaatc
    13921 atcgatgcat tgtagtttgc ttgcgagttc gcaccaccag gcatagaaat acgggatgaa
    13981 ctccacaaat tttgcgcagc cccgcccccc gacttctgag aagcattacc ttcggatttc
    14041 ccagattcag aagagacatg ggccactttt tctgtgaact ccactggggg atcatccggc
    14101 aaaagaaaaa tgacgatgat aaatataaaa aggagggtca ttcctagaat tttgaacgat
    14161 ctgtaatcaa cctctgtctt cccactaatg tctgaggatt tgaaaaattt cgattttagt
    14221 tttaaaaaaa attcgcaaac ccgatcttta atcacactca atttattcat tattgccata
    14281 ctgtcgcctc gggaatttga actttgattt tgttatttcc tcttagctcg atagaaaccg
    14341 gttttttaga ttccagatca gacttggaaa gagaaagccc tacgaggagc ggcttatttt
    14401 ttaatacctt cgtgtctgaa ataaaaagcc cgttgatgac tcttgagacc ccattttgag
    14461 tgacccagaa ttgatctggc tttacttgtt gagtttcaaa acccgagtaa gtaatctttg
    14521 catcaataag tacaaagtca ttttgcgaga taccaactct ttggaccttt aggataaggg
//
LOCUS       DATBTE010000288         3006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_857661, whole genome shotgun sequence.
ACCESSION   DATBTE010000288 DATBTE010000000
VERSION     DATBTE010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857661"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..426)
                     /locus_tag="VIG33_10710"
     CDS             complement(205..426)
                     /locus_tag="VIG33_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271347.1"
                     /translation="MNKSQTENPRIQYFEKADNDRHREFINSQSNCVLCGTVLELTHA
                     VDHIVGHIREEAHCPSCEVRTRARFHPLN"
     gene            586..1461
                     /locus_tag="VIG33_10715"
     CDS             586..1461
                     /locus_tag="VIG33_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271348.1"
                     /translation="MNYKIVFLFLIVLQTGCGVELRDRHQPEEINSPTELRIENEYSL
                     PIPLILSQQTVLHYDRLVLGPKAQLITQGLNVRIEVKELIAEDSIIRSFKPDQIAAAG
                     FNGRSGGHLELIAEKATGHLTLIMQGEKGGTGSNGAPADETLRGAKGADGLSAFFDYQ
                     NGNFLCIRKATSGSQGGQGLQGFPGGPGKEGGDSGTALVKITETENFDLQIQKNPGRG
                     GDGGPGGEGGPGGFGGEPGRETMPAYNGPDIHTYTAPGSPGIQGPPGFIGKTGDDGAA
                     EKSCVQWDDEVLRCE"
     gene            1644..2183
                     /locus_tag="VIG33_10720"
     CDS             1644..2183
                     /locus_tag="VIG33_10720"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8271349.1"
                     /translation="MDITTLQKAAREFGLLSLSKKTGLARSYLYKVVEGTSSPTIEAL
                     DKIADVLGFAIELHRMPLEDSVKDVSSKVAQGGSWKIHFFNFVDAFRRTKNPELVISP
                     PIESLDEKERVLLASMTVELCLKLGVEIPEWAKAQRPLKYPWFVAGVESLKPMAIVES
                     PISFKRNNIFVLENFLARA"
     gene            2228..2743
                     /locus_tag="VIG33_10725"
     CDS             2228..2743
                     /locus_tag="VIG33_10725"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HEY8271350.1"
                     /translation="MLTKEQIKELFYKLNQKLKKQNIRGEIGIVGGAVMCLVFNARVS
                     TKDIDGIFAPTQKIREFIKEIAEEEGVSENWLNDGAKGYIQGDFDKIEVMALSHLAIW
                     APQPEYMLAMKCISARIDTSDADDVRTLIQVLKLRSAREIFDIVARFYPKQRVPARTQ
                     FFIEELFEEGV"
     gene            complement(2790..>3006)
                     /locus_tag="VIG33_10730"
     CDS             complement(2790..>3006)
                     /locus_tag="VIG33_10730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8271351.1"
                     /translation="FDRLYRIDASRNPHSGGLGLGLSIVASIMKLHGGTVSVESEIGK
                     GSLFRLSFPNSSRLSNSRLFGKTNGAN"
BASE COUNT          861 a          604 c          753 g          788 t
ORIGIN      
        1 gccttgaggc ttgctgaaaa attggacgtt gggacagtct cgatcaactc ttggttcccg
       61 gaaaatttcg agcctggcca tcgtcagacc acctttggac aagtgaacca agggccatgg
      121 gaaagattct attcggatct caaagttttg acaggaccct aaaaccaaca aaattttggc
      181 gaaatcctgt cagaagttaa ttattcaatt cagaggatga aagcgagcgc gggtgcggac
      241 ttcgcaagaa gggcaatgag cttcttcgcg gatgtgtcca acaatatgat ccactgcgtg
      301 cgtcagttcc agaacagtgc cgcaaagtac gcaattgctt tgggaattta taaactcacg
      361 atgcctgtcg ttgtctgctt tttcaaagta ttgaatacgt gggttttctg tttgagattt
      421 gttcaaaggt cactccgtcc aaatatgttt tgatgcaatc aggtcactgc aagaactatt
      481 cacagcccaa ggtcggactt cgattcgtct ctcggacgta gaactcaggc aaacgaagtg
      541 aggcgatggt ctttggtttg taagtattcc tttcaggaga agtttatgaa ttacaaaata
      601 gtttttttat ttttaatagt tttgcaaaca ggatgcgggg tggaactccg tgatcgtcat
      661 cagcctgagg aaatcaattc acctacagaa ctgcggatcg aaaacgaata cagtttaccg
      721 attcccttga ttctttctca gcaaacggtt cttcactatg accgccttgt cttaggtcca
      781 aaagctcaac ttataaccca aggcctgaat gtgcgcattg aggtcaagga actgatcgcg
      841 gaggactcca tcattcgcag cttcaagccc gatcaaatcg ctgctgccgg attcaatggc
      901 cgaagcggcg gacatctcga acttatagcg gaaaaagcca cggggcatct taccttgatc
      961 atgcaagggg aaaaaggcgg cacaggttca aatggagcac cagccgatga aactcttcgc
     1021 ggggcaaaag gggcggatgg tttatctgca ttctttgatt atcagaatgg caatttcctt
     1081 tgcattcgca aagccacaag cgggtctcaa ggtggtcaag gtttacaagg ctttcccggt
     1141 ggccctggta aagaaggggg ggacagtgga acagccctgg ttaaaattac agagacagaa
     1201 aacttcgacc tgcaaattca aaaaaatcct ggccgcggag gagacggagg accaggcgga
     1261 gagggtggtc ccggaggatt tggcggtgaa cccggcagag agacgatgcc agcatataat
     1321 ggtccggata ttcacaccta tactgctcct ggctctcccg ggattcaggg acctccaggc
     1381 ttcattggca aaacgggtga tgatggagcg gcagagaaat cctgcgttca atgggacgat
     1441 gaagttttgc gttgtgagta gagaaaagga tttttgtttt ttgagttaag ctgatagagc
     1501 cgtaattaat atggagaata ttaattacgg ctcgtatcgc atcaactcta ctgtggatga
     1561 tttagaaatc taaagctaat atattcgatc tgaccatttg acaaaatgtc ctatatatag
     1621 gacaataagt ttgaggtgag caaatggaca taacaaccct ccaaaaagcg gcccgcgaat
     1681 ttgggctttt aagcctttca aaaaaaacgg ggcttgcccg ctcatacctc tacaaagtag
     1741 ttgaagggac ctcaagtcca acaattgagg cccttgataa aattgcagac gtcctgggct
     1801 ttgcaataga acttcatcgt atgccactcg aggattcagt gaaggatgtg tcaagtaaag
     1861 tagctcaggg tgggagctgg aaaattcatt ttttcaactt tgttgatgcc tttagacgta
     1921 ccaaaaaccc tgaattagtc atctctccgc ctatcgaatc attagacgaa aaggagagag
     1981 ttttacttgc atcgatgacc gttgagcttt gtcttaaact tggagttgaa attccagaat
     2041 gggcaaaggc gcagcgacca ttgaagtatc cgtggttcgt tgccggagtt gaaagtctta
     2101 agccaatggc gatcgtggag tcgccaataa gttttaaaag aaataatatc ttcgtgctgg
     2161 agaattttct agcacgagct tagcagggtt gtaggtgtct tcctttacta ggaacgagag
     2221 gttttgcatg ctaacaaaag aacaaatcaa agagttgttt tataaattaa atcagaaact
     2281 taaaaagcaa aacattcgcg gtgaaattgg aatcgtaggc ggtgccgtaa tgtgcctcgt
     2341 cttcaacgct cgtgtatcaa caaaggatat tgatgggata tttgcgccga cacaaaaaat
     2401 ccgtgagttc attaaagaga tagcagagga agagggtgtt tctgaaaatt ggcttaatga
     2461 cggtgctaaa ggttatatcc aaggtgactt tgataaaata gaagtgatgg cactttctca
     2521 tctagcaatt tgggctccgc agccagagta tatgcttgca atgaaatgca ttagtgctcg
     2581 tatcgatacg agtgacgctg atgatgtcag gactttgatt caagtgttga agcttaggtc
     2641 ggcaagagaa attttcgata tcgtcgctag attctatccg aaacaacgag tgccagccag
     2701 aacacagttc tttattgagg agctttttga agagggggtt taagttcttt aagagagcgc
     2761 gggctagaag ttcttcacta aataacttat taatttgccc cattagtttt tccaaaaagt
     2821 cggctgttgc tgagacgtga ggaatttgga aaggaaaggc ggaaaaggct ccccttgcct
     2881 atttcactct caacggaaac tgtgcccccg tgaagtttca tgatactggc cacgatggaa
     2941 aggcccaagc ccagtcctcc agagtgggga tttcttgaag cgtcgatgcg gtaaaggcga
     3001 tcgaat
//
LOCUS       DATBTE010000289         5543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_380242, whole genome shotgun sequence.
ACCESSION   DATBTE010000289 DATBTE010000000
VERSION     DATBTE010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380242"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VIG33_10735"
     CDS             <1..589
                     /locus_tag="VIG33_10735"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HEY8271352.1"
                     /translation="NGDVKSRKIQLQTKSGKKFFAIARLQSPTDLKGTALLSIIDKKG
                     EDQWLYTPSNQQVRRIVSVKKAGGILGSELSLQDLNSGDIAGAELQMIRSDSKDIVVE
                     AIPKKGTSPYQKVQTHFSPTQFVPLRTEYFQAQKLVKTVEFTDYKKVGDHLWRAQKII
                     VKNLKNGRGTELVLSQVKVNQDLKEEDFSVSALKD"
     gene            745..1608
                     /locus_tag="VIG33_10740"
     CDS             745..1608
                     /locus_tag="VIG33_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271353.1"
                     /translation="MPGTFSKFCQVFSLLTVLLFQNKLNADQFILNSVNLSLSNSEVN
                     QLIVSMCSLVSNHESQVQPKLRFHIHNWEILQNSGSNLSVKGACSQPVDLGVPTNQVQ
                     KGEDFNFNGKLDSSKAKFELRVELSHGVFNAVFVRESIFNKLSQRLQLQWQLQSVDFA
                     PSGVALSIVKTFPIASWYLEQTRAIQQTNYVLDRSVADREGNYYTQLRSLVDLHRMAM
                     WTKESGNVIHVNMASNSQFEWGFVPTEGSTSDAVFLKFGNRMMGCSNFLPVNRGDLTS
                     EECHMIISNLK"
     gene            1693..2961
                     /locus_tag="VIG33_10745"
     CDS             1693..2961
                     /locus_tag="VIG33_10745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEY8271354.1"
                     /translation="MQTSFNNENNQWARSSSNHNRWAMSSAPLRSVFSRIICSGSLRL
                     IDANGNQYDFGDGKPPFVSMHIHNPRLHYKLVLNPELYFGEAYMDGEIAIEKGSLKDF
                     FTIFFQNMAVAPAHSWLKILASLSYLRRPLQQLNTAQRSHKNVAHHYDLSVGFYDLFL
                     DSERQYSCAYFETGTESLEEAQKKKMAHILAKLLVEKKHHVLDIGCGWGGLSLYIARE
                     TGARVTGITLSQEQLLTARHAAHAQGLNGQVKFELLDYRHIEGPFDRIVSVGMFEHVG
                     INNYRAFFGQMKSLLASDGVALLHSIGRAHGPSSTSPWIRKYIFPGGYTPALSEVLPH
                     VERSGLYLTDMEILRLHYASTLKMWRERFEKNRDQAPVPLTEKFCRMWEFYLTCSETA
                     FEKLGLNVFQMQLATRQELVPLTRDYLLNR"
     gene            complement(3034..3279)
                     /locus_tag="VIG33_10750"
     CDS             complement(3034..3279)
                     /locus_tag="VIG33_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271355.1"
                     /translation="MKMILSAIVTLSAVAAFANPTTTTAPAHTGTAATTATTATTTKE
                     AKATTKAMTKEDAEKACAKETNKDACVKKHMGTDKKM"
     gene            3532..4005
                     /locus_tag="VIG33_10755"
     CDS             3532..4005
                     /locus_tag="VIG33_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3465 domain-containing protein"
                     /protein_id="HEY8271356.1"
                     /translation="MIKKIAFILGAVLSVSYFHANADEGWIPYQRGYRGAQNNEGDAK
                     IVQAVRNQRRLDFVEGSGMVVTQLLQDDKNGLQHQKWIVRLSSGATMEAVYNLDMCKR
                     VPLRVGDTVAMGGQFIWTNQGALLHWLHYDPRRNRPDGYVEVNGKVYCGEGDRPN"
     gene            complement(4015..>5543)
                     /locus_tag="VIG33_10760"
     CDS             complement(4015..>5543)
                     /locus_tag="VIG33_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY8271357.1"
                     /translation="KRSSTLQKVFILFAVTILFRIFFISCVGLADDEAYHWSWTKNLS
                     LSYYDHPAIIAWLEAITTSLLGDTRWGIRLPSFLCYLATATIGWKLARDVFGVWAANF
                     TVFLMMWTPLWGFGGYVASPETPFMLCWVAGAFIFWQGIREDEKQWPLKKTWLSLGIL
                     MGLGLNSKFIIALLAPGFGLYLLMTAKHRKDLLSKWPWMGMLLATLICLPIFIWNLHY
                     DWPGFKYQFHERHTTTEFSIGRWLQFFSAQILFYTPVAYALMLFTFGYAFIRRQEARW
                     RWLFCMTVPSILLFYAQPFWAEYKPHWSGPAFFFLAMGAGALWSEGIQWNEKVILKPF
                     NSKITGGILGFIIVLNLVIYLPFVYPWMPKVHRTLNPQSEWQPKWDLSNEFTGWLELG
                     DYVNRRQREIHAESGKKPFIASNRYETTAQTYFGTKQKVYMLSKTKSQYTVTQTPQEM
                     EALRSSTALFVTTDKYQTNPMEWAYFDGCNPEELKTYRHEEVARIFTVWICSNFQGIK
                     "
BASE COUNT         1581 a         1123 c         1253 g         1586 t
ORIGIN      
        1 aaatggtgac gttaaaagcc gaaaaattca actccagacc aaatctggga aaaagttctt
       61 tgccattgcg cggttgcagt caccgactga cctaaaggga accgcattgc tttcgattat
      121 tgataaaaaa ggggaggacc agtggcttta tactccctcc aatcagcagg tgcgacgaat
      181 cgtgagtgtc aaaaaggccg gtggaatttt gggatctgaa ctcagtttgc aggatttaaa
      241 ctcaggagac attgccggcg cggaactcca aatgatcaga agtgattcca aagacatcgt
      301 cgttgaagcc attcccaaaa aaggaacaag tccttaccaa aaagttcaga ctcatttttc
      361 gcccactcaa tttgtaccct taaggacgga atatttccaa gctcagaagc tagttaagac
      421 agtggagttc acggactata aaaaagtcgg tgatcatttg tggcgagccc aaaagattat
      481 agttaaaaat cttaagaatg gccgaggcac cgagcttgtg ctttcgcaag taaaggtcaa
      541 tcaggatctc aaagaagaag atttctcggt aagcgctttg aaggattaag ctgcttgttt
      601 aagaccggca atagatttgt aagcctcctg gatgtctcat tttaaatcaa tgtattccgc
      661 ttcctctcaa gtgatctggc acaactactg caactaagtt tcatcaagac ttaaagatag
      721 tatttgaatg aaataggagc aactatgcca ggaacttttt ccaaattttg ccaagtgttc
      781 tcacttttga cggtgctctt gtttcaaaat aaactaaatg ctgatcagtt tatattaaat
      841 tcggtgaatc ttagcttgag caatagcgaa gttaaccaac ttattgtttc catgtgctct
      901 cttgtcagta atcatgaatc gcaggtgcaa cccaagttga gatttcatat tcacaattgg
      961 gaaatcctcc agaattcagg gtcaaatttg agtgtgaagg gggcttgttc ccaacctgtc
     1021 gatctcggcg tgcccaccaa tcaagtacaa aagggtgagg attttaattt taatggaaaa
     1081 ttagattcct caaaagccaa gtttgaatta agagttgaac tcagccatgg agtgtttaac
     1141 gcggtatttg tcagagaatc aatttttaat aaactttcgc aacgacttca acttcagtgg
     1201 cagttgcagt cggtggattt cgccccttca ggagtggcat tgagtattgt caaaactttt
     1261 cccattgctt cttggtattt ggagcaaact cgtgcaattc agcaaaccaa ttatgtgctg
     1321 gatcgatccg tagctgatag ggaaggcaat tattatacgc agcttcgcag ccttgttgat
     1381 ttacatcgta tggctatgtg gactaaagaa tctggaaatg tgattcacgt gaacatggcg
     1441 tcaaattctc aatttgaatg ggggttcgtg cctaccgagg gaagtacttc ggatgcggtc
     1501 tttctaaaat ttgggaacag aatgatggga tgttcaaatt tcttgccggt gaacagggga
     1561 gatcttactt cggaagaatg tcatatgatc atttctaatc tgaaatagtt ttctttaaga
     1621 tatatttaaa attccttaga aaaaagatca cgttcataaa ttcccagtac agctccatga
     1681 taaaaatgag ggatgcagac aagcttcaat aatgaaaata atcaatgggc acgttcttct
     1741 tccaaccaca atcgctgggc aatgagttca gcgcctctgc gttcggtgtt ctcgcggatt
     1801 atttgctcgg gatctctgcg cctcattgat gccaatggca atcaatatga tttcggagat
     1861 ggcaagcctc cttttgtgag catgcatatt cacaatccca ggcttcatta taagcttgtt
     1921 ttaaatcctg agctttattt cggtgaggct tacatggacg gagagatcgc tatagaaaaa
     1981 ggaagtttaa aggacttttt tacgattttt tttcagaata tggcagtggc tcctgcgcat
     2041 agttggttaa agatcttagc ttctctctct tatctgcgtc gtcctcttca gcaactcaat
     2101 acggcccaga gatcgcacaa gaatgtggct catcattatg acctttcagt aggattctat
     2161 gacttatttt tagattctga aaggcagtat tcttgtgcct attttgagac gggaaccgag
     2221 tccttggaag aggcgcaaaa gaaaaaaatg gctcatattc ttgccaagct gcttgtggag
     2281 aagaagcatc atgttctcga catcggttgt gggtggggtg gactttctct ttatatcgct
     2341 cgtgaaactg gagctcgtgt gactggtatt accctgtccc aagagcaact cctcacagct
     2401 agacatgcgg ctcatgctca gggtcttaac ggacaagtga agtttgaact tcttgattat
     2461 cgtcacatag agggtccttt tgaccgaatc gtttccgttg gcatgtttga acatgtgggt
     2521 attaacaatt accgagcttt ctttggtcag atgaaatcat tgttggcgtc cgatggcgtg
     2581 gcgcttttac attcgatagg acgtgctcat ggtccctctt caaccagccc ctggattcgt
     2641 aagtatattt ttccgggagg atatactcca gctctttcgg aagttctccc acacgttgag
     2701 cgttcaggtt tatatttaac tgatatggag atcttgcgtt tgcattatgc ttctaccctg
     2761 aaaatgtggc gggaacgatt tgaaaagaat cgcgatcaag ctcctgtgcc ccttacggaa
     2821 aaattttgtc gaatgtggga attttattta acttgttctg aaacggcctt tgaaaaacta
     2881 ggacttaatg tttttcagat gcaacttgct acaagacaag aattggttcc attaaccagg
     2941 gattatcttt tgaatagata aaaaaaagcc actgcagaag agtgactttt ttagaaaaca
     3001 atttactaag caatttttag atgcttggta aaattacatt ttcttgtcag tacccatgtg
     3061 tttttttacg caagcatctt tgttagtttc tttagcgcaa gctttttcag cgtcttcttt
     3121 agtcattgct ttagtagtag cttttgcttc tttagtagta gtagcagttg tagcagtagt
     3181 tgctgcagtt ccagtatgag ctggagcagt tgttgtagtt gggttagcga aagctgcaac
     3241 agcagacaaa gtaacgatcg cagaaaggat cattttcata ggattctcct cgtttttgta
     3301 atttaggaaa tgccaccacg gcacgtttcc ttaaaatttg ttaattggaa tgagattaag
     3361 atttttatta taaatttcaa gcaagatata ggaatattta taagtctcag gcacaccatg
     3421 ttaagggcga atgtttttat attgtcattc ttaaacctca gaattttata gttgaaaaag
     3481 cggtggctgt cgctgaaggt aatccctatc acaagaaagc tgggggactt gatgattaaa
     3541 aaaatcgcat tcatattagg agctgttctt tctgtcagtt atttccatgc taacgccgat
     3601 gaggggtgga ttccctatca aaggggttat agaggggccc agaacaatga aggcgatgca
     3661 aaaattgtac aagccgttag gaatcagcgt cgcctggatt ttgtagaagg ttcgggaatg
     3721 gttgtaactc aactcctcca agatgataag aacggtctac agcaccaaaa atggattgtg
     3781 cgtctctcca gcggagccac catggaagct gtttataatt tggatatgtg caagcgggtt
     3841 cccttgagag tgggagatac cgttgctatg gggggacaat tcatttggac gaatcaaggg
     3901 gctcttctgc actggctgca ctatgatccc cgtcggaacc gccccgatgg ttatgtagaa
     3961 gtgaatggga aagtgtactg cggtgaagga gatcgtccca attagtttcc atccctattt
     4021 aattccttgg aagttgctgc aaatccacac tgtgaagatg cgtgccactt cctcgtgacg
     4081 ataggttttg agttcttcag gattacaccc atcaaagtag gcccactcca taggattggt
     4141 ttgatattta tcggtagtca caaacaatgc cgtcgagctt ctcagggctt ccatttcttg
     4201 tggagtttga gttacggtgt attggctctt ggttttactc agcatataga ctttttgctt
     4261 tgttccaaaa taagtctggg ctgtggtttc atagcgattg cttgcaataa aaggtttttt
     4321 accgctttcc gcatgaatct cgcgttggcg gcgattcaca taatcgccaa gttccagcca
     4381 tcctgtgaat tcattgctaa ggtcccattt tggttgccac tcagattggg gattcagggt
     4441 acgatgaact ttcggcatcc agggataaac aaaaggtaaa tagatcacta agttgaggac
     4501 aatgataaat cccaaaattc ccccagtgat tttactatta aaaggcttca ggatcacttt
     4561 ttcattccac tgaattcctt ccgaccaaag agctcctgcc cccatggcta aaaagaaaaa
     4621 agcgggtcca ctccagtggg gtttgtactc tgcccaaaaa ggctgggcgt agaaaagtaa
     4681 aatgcttgga actgtcatac agaaaagcca acgccagcgt gcttcttggc gacgtataaa
     4741 ggcataacca aaggtaaaga gcattaaggc ataggcaacc ggcgtataga acaaaatctg
     4801 tgcgctgaaa aattgcagcc aacggccaat actaaactcc gtggtcgtgt ggcgttcatg
     4861 gaattgatac ttaaatcctg gccaatcgta atgcaggttc caaataaaaa tgggaagaca
     4921 aatcaaagtt gccagaagca tgcccatcca cggccatttc gacagcaaat ccttacgatg
     4981 tttcgcagtc attagcagat aaagaccaaa acccggcgct aaaagcgcga taataaattt
     5041 agaatttaaa cccaaaccca tgagaatgcc gagggacagc caggtctttt tcaaaggcca
     5101 ttgtttttca tcctcacgga ttccttgcca gaagataaag gctccggcca cccaacaaag
     5161 catgaagggg gtttccgggg acgccacgta gccgccgaat ccccacagcg gtgtccacat
     5221 catcaagaat acagtaaagt tcgccgccca aacgccaaag acatctcggg ccagtttcca
     5281 cccaatcgta gccgtagcga gatagcatag aaaagaaggc aagcggatgc cccaacgagt
     5341 gtctcccaag agggaggtgg ttatggcctc tagccatgca attatggccg gatgatcgta
     5401 gtaagacaaa gaaagatttt tggtccaact ccagtgatag gcttcatcgt cagcgagtcc
     5461 tacgcaggaa ataaagaata tgcgaaataa gatagtcaca gcaaaaagaa taaaaacttt
     5521 ttgcaacgta ctggatcgtt ttc
//
LOCUS       DATBTE010000290         5851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1609654, whole genome shotgun sequence.
ACCESSION   DATBTE010000290 DATBTE010000000
VERSION     DATBTE010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609654"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..465
                     /locus_tag="VIG33_10765"
     CDS             220..465
                     /locus_tag="VIG33_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEY8271358.1"
                     /translation="MKKDLHPKINTVVFKDISCDFSFLGTSTMHSSENVKWEDGKEYP
                     LIKVEISSESHPFYTGKQRVLDTEGRIDRFKKKYGKK"
     gene            596..1672
                     /gene="prfA"
                     /locus_tag="VIG33_10770"
     CDS             596..1672
                     /gene="prfA"
                     /locus_tag="VIG33_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEY8271359.1"
                     /translation="MFSKLAEVESRYEQVNLQLQRPDIASDQKKYRAFMKELADLEKI
                     VLVYREYKTKTSNLKASKELLTAEQDSEMRELIREELKELETNIPRLEQELKILLIPK
                     DPNDEKNIILEIRAGAGGDEASLFADELFRGYAHFAANQGWKVEMLSYSEGNVGGAKE
                     VIASISGEAVYNKLKYESGVHRVQRVPKTETAGRIHTSTVTVAVIPEVPINEIKINMN
                     EVRIETMRSQGSGGQSVNRTESAVRVVHLPTGIDVKCQEGKSQASNKERAFQILYAKL
                     QQIEDDKVLKEASDARLEQIGTGDRSERIRTYNFPQTRITDHRIGLTIHQLDQVMSGS
                     FELLIDPLIANFQAEALKQQTSAG"
     gene            1672..2577
                     /gene="prmC"
                     /locus_tag="VIG33_10775"
     CDS             1672..2577
                     /gene="prmC"
                     /locus_tag="VIG33_10775"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY8271360.1"
                     /translation="MKLKEVLDKTTQFFKDKNIESPRLDAELLLAHGLKLQRIQLYLK
                     FDQPLTDGELVTCRELVRRRALGEPVAYILGVKDFYGFSFQVNSAVLIPRPETEHLVE
                     AALEWVKEQGFSQDDHKMNSYNIVDLGSGSGCIGLTLLKKIPQAKLVAVDISEKALEI
                     AKVNAEALEVVDRVRFVNRDAGNVNEVLAALKDFTGTNKIHILVSNPPYIALEDKDVE
                     ENVKKFEPEIALFAQENGLKFLRDWSQKYRLYLNEKAIVLMETGMTQGPAMQKHFEDL
                     GVFNKVDVIKDLAGLDRVIRGSKYG"
     gene            2570..3823
                     /gene="murA"
                     /locus_tag="VIG33_10780"
     CDS             2570..3823
                     /gene="murA"
                     /locus_tag="VIG33_10780"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY8271361.1"
                     /translation="MDKMLVTGGATLCGTVQTSGAKNAALPILFSTLLAEGEHIFKNV
                     PQLKDIESTAALLESLGCETRRDGDTFRVKVSKLQSIEAHYDLVRKMRASILCLGPIL
                     TKYGEAVVSMPGGCAIGTRPIDLHLEGMAALGAEITLKEGYVHAAAKRLQGATILFET
                     CTVGGTENVMMAATLAKGRTILENAAKEPEIVDLAEYLIKMGAKITGHGTSIITIEGV
                     DKLIPSEHSIMPDRIEAGTLLIAGAITKGEVTVEKCVPQHLEALVLKMREAGFKIEQS
                     KTSLTILKAKSWEAVDVTTAPHPLFPTDLQAQFMALMAVANGTSIISETIFENRFMHV
                     QELIRLGSDITPKTRVAVVRGKPDGLTAAPVMATDLRASASLVLAGLVAKGITAVGRI
                     YHLDRGYENLESKLQSLGAKIQRAE"
     gene            complement(3988..4209)
                     /locus_tag="VIG33_10785"
     CDS             complement(3988..4209)
                     /locus_tag="VIG33_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271362.1"
                     /translation="MIRNALIALYILAVLAISAVSRAESKISVNIIEPKHPVRPVFNP
                     YAADPLPKLGHLGHNAMTILSIGTKGVRK"
     gene            4450..>5851
                     /locus_tag="VIG33_10790"
     CDS             4450..>5851
                     /locus_tag="VIG33_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271363.1"
                     /translation="MSRTQIYWIVKTTSGQTKGPYTTEAFLKLIGEGVFSGSEMVSRY
                     PDGQWTLLSHETQFYDKLLEVLENSVEEVKKSSDNVNAETVLGPVPEHLKNPLGHQRT
                     ILEKSDALQTNLNSAPTSKKAQPTPSTDGPVIDLKNISQAQQQALIKTAKWPLILVIL
                     ALLLVVYVLLPQVVDVEEKISLIAPKKGAAAMSDQQIKEKYGTAIVAIEADTIESYMV
                     AQNHLVSIVEGAPSNLEVRGLLCVVYKELWPFAKQDANDLRAISSVTQTTRALNAVGF
                     FGSICEIVNLETSGHYKEARGVVESALENADHFSLLPVLYQFKAELLEADKDYLNATP
                     YYEKAAQLWARWLRPQVKSGVMAAIQNQGPKAAQFFRNVLTKNPNHREAKIRAGILEY
                     KAFKQEDNAFKLLKSALSSTTRVQRDLEAEGYFYLAELYLSKSEKSSALSAAQSAYKL
                     NPNNSQYKQMVLRLGGS"
BASE COUNT         1775 a         1155 c         1327 g         1594 t
ORIGIN      
        1 gttcaaggta ccaaaacgaa tacggacttc ttgaaggcta tgtcgggcaa ctaaaggtag
       61 aggtcgctaa atattataag ctattgattt ctaagatctt tttagatctt agaacgcgct
      121 taaaaaatta gcgatttctg atagacatta cttaaccccc tatgttacaa aactgaacct
      181 tacggccaat aatattcaat atttagaagg aattaggtca tgaaaaaaga tcttcatcca
      241 aaaattaata cagttgtttt caaagatatc tcttgtgatt tcagcttctt gggaacttcc
      301 acaatgcatt cttcagaaaa tgttaagtgg gaagacggca aagagtatcc tctgatcaaa
      361 gttgagatct cttctgaatc tcatccattc tacactggta agcaacgtgt attggataca
      421 gaaggccgta tcgatcgttt caaaaagaaa tacggaaaaa aataaatacg catacctcca
      481 ctatctgctg gaggactatt gcattagcaa tgcggtccaa tttgaggccg catttgtttt
      541 ttctggatgt tttcttgatc tattctcaga ccttttcacg ctagaggtat gaaagatgtt
      601 ttcaaaactt gctgaagttg aatcccgtta cgagcaggta aatctccaac tgcaaagacc
      661 tgatattgct tctgaccaga agaaatatag agcttttatg aaagagttgg cagatcttga
      721 aaagatcgtt ttagtttatc gtgaatataa gaccaaaacc tcaaatctta aagccagcaa
      781 agaacttcta acggctgaac aagacagtga aatgcgggag ttgattcgtg aagaattgaa
      841 agaactcgaa accaacattc caaggcttga gcaagagctt aaaattctcc ttattcctaa
      901 agacccgaac gatgagaaga atattattct ggaaatccga gctggagctg ggggcgacga
      961 ggcttctttg tttgctgatg aacttttccg tggatatgcg cattttgcgg caaatcaggg
     1021 ttggaaagtg gaaatgcttt cttattccga aggcaatgtg ggtggagcta aagaagttat
     1081 tgccagtatt tctggcgaag cagtctataa taaattgaag tatgaatctg gagttcaccg
     1141 ggttcagcgt gttcctaaaa cagagactgc gggtcgcatt cacacttcaa ctgttaccgt
     1201 ggcagtaatt cccgaagtgc cgatcaatga aattaaaatc aatatgaacg aagtgcgcat
     1261 cgagactatg cgttctcaag gctctggtgg acagtcagtt aacagaactg aatccgcagt
     1321 tcgcgtagtc catttgccta cagggattga tgtgaagtgc caagagggga agtctcaagc
     1381 ctccaataaa gaacgcgctt ttcagatctt gtatgcgaaa cttcaacaga ttgaagatga
     1441 taaagttctt aaagaagctt ctgatgctcg cttggagcag atagggacgg gcgatcgttc
     1501 ggaacggatt cggacctaca atttcccgca aactcgtatt acggatcatc gaattggact
     1561 taccattcat cagctggatc aggtcatgag cggttctttt gagttgctga ttgatcccct
     1621 cattgctaac tttcaagcag aggcattaaa acaacaaacc tctgccggat aatgaagcta
     1681 aaagaagtcc tcgacaaaac tactcagttc tttaaagata aaaacattga gtctccgcgt
     1741 cttgatgctg aacttttgtt ggctcatgga ttgaagcttc aacgcatcca actctatctt
     1801 aagtttgatc aacctttgac ggatggtgaa cttgttactt gccgtgagtt ggttcgtcgc
     1861 cgagccctgg gggaacccgt tgcttacatt ttaggggtaa aggattttta cggatttagt
     1921 ttccaagtaa actctgctgt gctgatacca cgacccgaaa ctgaacatct cgtggaagca
     1981 gctttagaat gggttaagga gcaaggcttt agccaagatg accataaaat gaactcttac
     2041 aatattgttg atttgggttc aggctctggt tgtattggat taaccctttt aaaaaaaata
     2101 ccccaagcaa agcttgtagc tgtcgatatt tcagaaaaag ctcttgaaat tgccaaggta
     2161 aatgctgagg ctcttgaagt tgtggatcga gtccgctttg taaatagaga tgccgggaat
     2221 gttaacgagg ttctggcagc tctaaaggat tttactggaa caaataaaat ccatattctt
     2281 gtctccaacc ctccctatat tgctcttgaa gacaaagatg tagaagaaaa tgtaaaaaaa
     2341 tttgaaccag aaatcgcact ctttgctcaa gaaaatgggt taaagttttt gcgtgattgg
     2401 tctcagaaat atcgactgta cttgaacgaa aaagccattg ttttaatgga gacggggatg
     2461 actcagggac cggcaatgca aaagcatttt gaagacttgg gtgtttttaa caaagtggac
     2521 gttatcaaag atctcgcagg tcttgataga gtcattagag gaagtaaata tggataaaat
     2581 gctcgtaact ggcggtgcaa ctctttgtgg aacagttcaa accagcggtg ccaagaatgc
     2641 cgctttaccc attctctttt ccactctcct tgcggaagga gaacacatct ttaaaaacgt
     2701 tccacagttg aaagatattg agtctacggc tgctctttta gaaagcttag gttgcgaaac
     2761 acgcagagac ggcgacacat ttcgagtcaa agtaagcaag cttcaatcca ttgaagccca
     2821 ttacgatctg gttcgtaaaa tgagagcgag cattttatgc ttaggtccaa tccttactaa
     2881 atacggtgaa gccgtagttt caatgccagg aggctgtgct atcggcacac gtccgatcga
     2941 tcttcactta gaaggtatgg cggcgctggg cgcagaaatt actcttaaag aaggatacgt
     3001 tcatgcagcg gcaaagcgct tacagggcgc taccattctt tttgagactt gcacggtggg
     3061 tggaacagaa aatgtgatga tggcagccac tctggcaaaa ggaaggacaa tcttagagaa
     3121 cgctgctaag gaaccagaaa tcgtcgatct tgcagaatac ttgataaaga tgggcgcaaa
     3181 aatcacaggc catggaacca gtattattac gatcgaaggt gttgataaac ttattccgtc
     3241 tgagcacagc attatgccag atcgtattga agcaggaact ctgctgatcg caggcgccat
     3301 tacaaaaggt gaagttactg tagaaaaatg tgttcctcaa catctcgagg ccttggtgct
     3361 aaaaatgcgc gaagcgggct ttaaaatcga gcaaagcaaa acttccctca ctattcttaa
     3421 ggcgaagagt tgggaagctg tagatgtgac tacggcccct catccacttt ttcctacaga
     3481 tctgcaggct caatttatgg ccctgatggc agttgccaat ggcacgagta ttatcagtga
     3541 aacaatattt gaaaatcgtt ttatgcatgt tcaagaactc atccgtttag gatccgatat
     3601 cactccaaaa acaagagttg cagtagtacg aggaaaacct gatggcttaa cggcagcccc
     3661 cgttatggca acggatctca gagccagcgc ttcactggtg ttggcaggct tagtagccaa
     3721 aggtataact gcagtaggtc gcatttatca tttagatcgt ggctacgaaa acttagaatc
     3781 caagctccaa agcctgggtg cgaaaattca aagagcggaa taacaagttc tacttgcctc
     3841 tccagcgttg ctattgctta gcgaaaaata aattaaattt tgaataaaaa aagcctcgtt
     3901 gggagggggg tcctttcgag gcttttgaag ggattaaaaa aatgtctcag ccccgagggg
     3961 tggactgaga catctacgga gtgggagcta tttacgaact cccttagtgc caatactgag
     4021 tattgtcatc gcgttatgac cgagatgtcc taacttaggg agggggtccg cagcgtaagg
     4081 attaaataca ggtctaacgg gatgttttgg ttcaataata ttaactgaga tttttgattc
     4141 agcacgcgat acagcagaga ttgcgagaac tgctaaaata taaagtgcta tgagtgcatt
     4201 gcgaatcatt tgattctccc agtgaaagac ttctaaagca acactcgtgc caacctcttt
     4261 tgaatctaag catgagttaa atttaatttg aatctagcaa aagagatcaa aaaccctatt
     4321 tggtaattcc acattgggtt ttctgattcc aatttgggac taaagtctaa ggactttggt
     4381 agtgacctga atatgatctc gagcaagatg atactctgtg tgtaaaaagc gaaatataat
     4441 caactatcta tgtcacgcac tcagatctat tggatagtta aaactacatc gggccaaacc
     4501 aagggtcctt acacaactga ggctttcttg aagctgatcg gagaaggtgt attttctggt
     4561 tcagaaatgg tttctcgtta tccagatggt caatggacat tactttccca tgaaactcag
     4621 ttctatgaca aattgctcga agtgttagaa aactctgtcg aagaggtgaa gaaatcaagc
     4681 gacaacgtca atgcagagac tgtcttgggg ccggtaccag agcacttaaa gaatcctctg
     4741 ggacatcaaa gaacaatttt agagaagtca gacgcgcttc agacaaactt aaattccgct
     4801 cccacttcca agaaagctca gccgactcct tcaaccgacg gacccgttat tgatctgaaa
     4861 aatattagtc aggcgcaaca gcaagcactt ataaagactg ctaagtggcc tcttatttta
     4921 gtgatcctag cacttcttct tgtggtatat gttctgttgc cccaagtcgt cgatgtagag
     4981 gaaaaaataa gccttatagc tccgaaaaaa ggagctgcag cgatgagtga tcaacaaatc
     5041 aaagagaaat atggtactgc tattgtcgcc attgaagcgg ataccattga atcttatatg
     5101 gtggcgcaaa atcatttagt ttctatagtg gaaggtgctc ccagcaatct cgaagttcgt
     5161 ggtcttttgt gtgtggtata taaagagctt tggccgtttg cgaagcaaga tgccaatgac
     5221 cttcgagcaa tttcgagcgt tacccaaact acccgggctt taaatgcagt gggatttttt
     5281 ggaagtattt gtgagattgt gaatctagaa acttccggtc actacaaaga ggctcgggga
     5341 gtagtggagt ccgcactcga gaatgcagat cacttttcac ttttgcccgt gctttaccag
     5401 tttaaggcag agttattaga agctgataaa gattatttga atgcgactcc ctattatgaa
     5461 aaggcggcac aactttgggc acgatggctt cgccctcaag tcaaatccgg cgttatggcg
     5521 gcaattcaaa atcaaggccc aaaagccgct caatttttta ggaatgttct tactaaaaat
     5581 cctaatcatc gggaggcgaa aattcgggca ggaattcttg agtataaggc ctttaaacaa
     5641 gaggataatg cttttaaact tttaaagtct gccttgagct ccactacgcg agtgcaaaga
     5701 gatcttgagg ctgaaggcta tttttacttg gccgaacttt atctcagcaa gagcgaaaaa
     5761 agttcagcgc tgtccgcggc tcaaagtgct tataaattaa accccaacaa ttctcaatac
     5821 aagcaaatgg tattaaggct ggggggctcg g
//
LOCUS       DATBTE010000291        17101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1541978, whole genome shotgun sequence.
ACCESSION   DATBTE010000291 DATBTE010000000
VERSION     DATBTE010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 17101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17101
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541978"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VIG33_10795"
     CDS             <1..1077
                     /locus_tag="VIG33_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8271364.1"
                     /translation="RKIKVVISDLHLGKGRILENGGSNSLEEFYYSEKLVEFIHYYSS
                     GIYRDYEVELIINGDFLNCLQVDYKGHFLTVITEAVALEVIKSIIKGHEKVFKAMADF
                     AVKSGNSITYVVGNHDQSMLWPSCRAYLNQVVGSPIRFKNIVYFFDGVHVEHGHMHEA
                     ANRMDPKKFFLKKDLPEPILNLPFGSHFFIELVLKVKQRYPHVDKIRPFAKMVKWALF
                     NETRMLVGSFYSLIVYFGKALLVKDPRRNWPFRRILQVILESAIFPDLSEAARRVLVD
                     DRVHTVIFGHTHVYQYRQWSTDKEYFNTGTWTELTSLDIVSLGKITKLTYVLIEYPED
                     GGRPRGRLKEWRGYHRIEEDVAVS"
     gene            1236..2171
                     /locus_tag="VIG33_10800"
     CDS             1236..2171
                     /locus_tag="VIG33_10800"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY8271365.1"
                     /translation="MTNHDEDSKDVSEKTSVVSSDTFKGRIKAADNAPPVVVVLIGPP
                     GYVGKQYPISSNDIVIGRSVETQVYIDDKSLSRSHAKFAVVAGEVSIIDLGSTNRTIV
                     NGNALPPLAPCKLKNNDQIKTGNVIFKFLEKGNIEALTNQTMYEKANRDALTGAHSKG
                     SLLEKGPEAVKRSELLSEEMSVIIFDIDHFKKINDTYGHPAGDFVLKELANIVSSKLI
                     RSNDYFARYGGEEFVLILSGGTAKMASEIAERIRQTMENHAFVYEGKTIPVTISLGVV
                     TKSTNDTDWPEIFDRADKAMYASKQAGRNRVTVAQ"
     gene            2220..2492
                     /locus_tag="VIG33_10805"
     CDS             2220..2492
                     /locus_tag="VIG33_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271366.1"
                     /translation="MKSFLVVVLGVALFSVCSWAQEGNDQRTVSECASELKIKVWWDS
                     HKYKAKKYCEEYSQASINCAINLMQAKQLTYTNFEKALKDCARTRK"
     gene            2864..3481
                     /locus_tag="VIG33_10810"
     CDS             2864..3481
                     /locus_tag="VIG33_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8271367.1"
                     /translation="MLRDLLKTDLELVEEVKTGNRRSFSELVKRHQKGLLRMSLRFVK
                     DLDAAEDVVQESFIKAYEKLHSFEGRASFKSWLFQIAVNNARNRIRDYRKNMVDIENV
                     QLAVGAEVEGGMVHDAVAGVLREEVDKLPDRQRTALVLRVYEDLSFKEIAEIMGCPYD
                     TAKANYRHALMKLREIFEEQQDLKSWTEEVGGFLGETSQYVEAEG"
     gene            3478..3690
                     /locus_tag="VIG33_10815"
     CDS             3478..3690
                     /locus_tag="VIG33_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271368.1"
                     /translation="MKKKQLLGSVLDEVQLPHNKTYYDRMHDKIMARIEDTEMEAPIR
                     PARRSFEKSKQLLREQWRHWLAPHEP"
     gene            3862..4317
                     /locus_tag="VIG33_10820"
     CDS             3862..4317
                     /locus_tag="VIG33_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HEY8271369.1"
                     /translation="MKSFIVALATLGYVISANAASADSLKGTGVYGDAGCGLGSMVFG
                     NKEGIVQVVAATLNGTGMQTFGITSGTSNCGPGLFSSAEINSFMQSNAVALENDIARG
                     QGETLSTLNNMLGCSSQFGDTLQKNYKNIYSPGINPAQKIVAMAESCQG"
     gene            4329..6236
                     /locus_tag="VIG33_10825"
     CDS             4329..6236
                     /locus_tag="VIG33_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEY8271370.1"
                     /translation="MTKKGLTVMGLSLFLLIFFSEQKAMASEVLDRAQREHWFEDRQW
                     LRLVQYHPHFFGGYKSSGESDKFFLAPKGKSRPDLELTATLLALQENTRTLRSQDGRF
                     EDPVGCVFPARKLWLEKKAGIKFPNPQCERFNRFKEILQPQSLTYVFSSYYLNNPASA
                     FGHTFLRVNKAPSAINGERFELADYGVGYAAVKTSENPFVYSFLGVSGLMPGAFDINP
                     YYYKVREYNDFESRDLWEYDLTFTPEEVEMVIAYIWELLDVNFNYHYFTENCSYRILS
                     VLEVARPSLELTDHLKSQVMPADTVQTLFQQQGLVGRIHFRPSIRATFEQRYRGLSDE
                     GKSRLRGFARNESIAEMIQDLEPVEKRGSLDTAMDYLDYRYSKEILRKEEKYIFKKDV
                     LRARAELGGASALLKLSTAESEAPHEAQGSRRWGFGYREWNKRNFYLLEMKAALHDLL
                     DPKLGYPPTAEITMGYLGLSLDSTSQELALDRAILYEVVSLSPLNDFNRDVSWRLKFS
                     LERGFENNCLGLCRWTELSGGSGLTTTVWSDLDLSVWLRLTTQTSTDFQGDTWRIGAG
                     PGLSARWNRNAWAVMAEIYDRYDYKGLNPEFRQSSLGINWSLNKNLSLRFRGAEENSL
                     RLMEARLLYYY"
     gene            6264..7586
                     /locus_tag="VIG33_10830"
     CDS             6264..7586
                     /locus_tag="VIG33_10830"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEY8271371.1"
                     /translation="MTNVTVFWFRRDLRLDDNAGLFHALKEKEEKVLPFFIFDTDILA
                     GLDKADARVTFIYQRVAELKERLQHLKSDLLIRSGRPLEVFKNLHKEKHISAIYLNHD
                     YEPSAIKRDEEIRQWAQTQEIEFKSFKDQCLFEKDEIVTDTGKPYRVFTPYKNKVLSH
                     LPKHVFQFYDNESVSSAYVKVQEVERVLTLKDLGFAMSSLHFPSKEIPLSVIKNYAKT
                     RDFPANDNGTTHLGIHLRFGTISIRKLAFLARKESSVFLSELIWRDFFMQILWHYPQV
                     EKRSYKSAYDKIAWRTSKKDFERWAHGMTGYPLVDAGMRELQETGHMHNRVRMVAASF
                     LCKHLLIHWSEGEKYFAKKLLDFDLSANNGNWQWVAGSGCDAAPYFRIFNPDAQAKKF
                     DPQKEYIKKWIPEFNTSRYPAPMIQHEEARGRCLQAYASVNSYKEFIP"
     gene            complement(7576..8493)
                     /locus_tag="VIG33_10835"
     CDS             complement(7576..8493)
                     /locus_tag="VIG33_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY8271372.1"
                     /translation="MKTQFSSLKILSKLLSLMILTVSATKALAMDFVPYTIYGEDNRN
                     NIYTAPSTIQALGRSVPGQFSKDVLMNKGGGYSVESTTLGKRHCSSVRFGEEFLGPRC
                     TGFLIAPNVVATAGHCVKDANDCANYYWAFDFKLKSAGDTSYTRVPAENVYTCKRILA
                     QKFEFLDGIDFAIIQLDREVKGRDALPLDFTSDTPVGTALFVLGYPSSTPLKYTDSGV
                     ISKNLEKIYYSNLDIFGGNSGSPVFDVSTGKITGIISEGNGDWVWNDGNTCKIAKVCS
                     EGGTCGHSITSKINIVKDEFEKALAKSKE"
     gene            complement(8591..9634)
                     /locus_tag="VIG33_10840"
     CDS             complement(8591..9634)
                     /locus_tag="VIG33_10840"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEY8271373.1"
                     /translation="MDVLKATVTISSALLLSSCQVSYLVKSAYNQLSIMNSQETLDEA
                     LKNPKLTPEQKRKIQLAQEAREFAEQSLSLKATKNYTSFVLLDRPYVTYVVSAAPKWK
                     LEAYKWHYPIVGAMPYKGFFNELDAKKEEENLKQENLDTYMRGVSAYSTLGWFKDPLL
                     SSMLQYKDYDLVNTIIHETVHATLYIKHEADFNERMAVFMGNKGTEMFYLKKEGSESP
                     TLLTVRNENADDKIFSAWLSTKLKKLEKWYQNVPAHEQTDNKRQQQFVQLQEDFKKEM
                     LPQLKTESYKKFQNIKLNNARLLVYRTYMQDLGDFEKLYEKTGGDFKKFLEECKAFEK
                     EKNPVEALKDRVS"
     gene            complement(9622..10194)
                     /locus_tag="VIG33_10845"
     CDS             complement(9622..10194)
                     /locus_tag="VIG33_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY8271374.1"
                     /translation="MKKTFQNLTILISLILPLNSHAMFTEMGLTYGRKKTTFDANNNY
                     DSESTTGSVSFYFAEMLALELSYTEAVGIQKQQASSLDTPRTIYQKTQIIGTDLIVVF
                     ADKKALFQPYIKGGAAQISRRQEIKDGSFPTQILEPDVAIAPSYGLGLKIALTESFGF
                     KASYSIWQTPIGGGAKTNDDAVSAGVTWMF"
     gene            complement(10260..11108)
                     /locus_tag="VIG33_10850"
     CDS             complement(10260..11108)
                     /locus_tag="VIG33_10850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY8271375.1"
                     /translation="MNLLRALLLSLVVHFVLVLVLDKGAPYFEPKPLSVTTIDLLERP
                     REQSNKNKIANKERQVVRQALAPKQDLKDDDTDLARFLSAEKQRVRKEMQAAVSGMTK
                     NRTTGQKEKSETKEKKYDPEGADIAKNLKEYSQSAENAPATVGEALPQDVSIGNFTAL
                     NTDRYVYYSFYARIEELVRFRWESRVRKAVDSFDRNYVLGVVGRRNWVTSVDIWLTKD
                     GRYHSAHILKESGINHFDQAAVLAFKEAGMFPNPPQDMVEDDGFIHLKYSFNVSFNPG
                     AVVYSE"
     gene            11206..11715
                     /locus_tag="VIG33_10855"
     CDS             11206..11715
                     /locus_tag="VIG33_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY8271376.1"
                     /translation="MAKVINITSRVKSQNSLMNNNKSFEAPVLDMTEAREEILNKERR
                     EVKRTILTEFIGTYAVLPERGLMKVTLYDISENGIAFDLEPQEGMFRDGEEVAIRLYL
                     NHTTYFPFNVNVIKSRPMEEEGVIRHGAQFIKGTVNDVALHHFVKFIENVSAGLRKDE
                     GDILVSNIS"
     gene            11839..13161
                     /locus_tag="VIG33_10860"
     CDS             11839..13161
                     /locus_tag="VIG33_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY8271377.1"
                     /translation="MSKSKRRKIVVDTNVILFDAQAILRFGEADVHIPISVIEEIDKF
                     KRDQGENGRNARQFSRFVDVLRAKGSLAQGVQLDNSETMVYISTDLMLAGMPPEFDQI
                     KADNRILNTALALQKQYPRLKVELITKDINLRIKADVYGIFAKDYEPTDVSQEDMYEG
                     YLELEISPEQIDHFYKDKKHVHTGTQKLYANQYVIMKDSSNPNHSAIGRFSHAESAIV
                     PLINPPDSIWGIHARNVEQAFALDSLLNDEILFVSLVGKAGTGKTLLALAAGLFKTLD
                     EGRFQRLLVSRPIFPMGKDIGYLPGDIEQKLNPWMQPIFDNVEFLMGSDKKASGRAQE
                     LINQGMLNIEPLTYIRGRSIPKQYLIVDEAQNLTPHEIKTIVTRAGRGTKVILTGDVY
                     QIDNPYVDSANSGLTYAVERFKGHPIAGHVTLTKGERSELAELAANIL"
     gene            13251..13664
                     /locus_tag="VIG33_10865"
     CDS             13251..13664
                     /locus_tag="VIG33_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY8271378.1"
                     /translation="MSINKGRDREVGFKAVLEAKVTDQMVRQFAEMSGDFNPIHLDDE
                     YAKTTRFGRRIAHGMIVGALISRTLNEKLGSGGVYLAQNLKFVNPVFIDDTIIIEITV
                     TGLRKEKGIATLDTIAKKENGEIVVKGDAIIMMAQ"
     gene            13785..15149
                     /locus_tag="VIG33_10870"
     CDS             13785..15149
                     /locus_tag="VIG33_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271379.1"
                     /translation="MKQLKTFLLMGMTVVAVRAQAEDLAAGQANALPYIDQDFACITQ
                     QQAQKYTNDFSIDINSFGGLELCNSNVDAKKLFNDLQIIEQGQFAAVNSNILVKGYIP
                     ADQYYSWMKNQTRGMNRGNDIPYATAYNRMGYFTMQDGWAKISTLGRVGVVLHEARHT
                     GGYRHYPCKQGPYMGSSLDGCDQDFTQGGSHAIEMEYYARVTVAGQNFHPVYKAMARL
                     MAMGRASFVFNTSPIQKREAVLALEEQAGPVMFDGTRKVQREGTDSVGQLKRTSFGAV
                     IFNGLQAVALDVYEMTGFRSKTNDDYSYFKLLGMNQMGSLRDFEEYDVNQKRYVTAVT
                     ASNQLATYNFPKGSWNSPATLPLTVERTASTLPTGEKGYFLMNNVGDIYSVNADNQQV
                     TSTGKKWDAQVQSAALFGGKILLLKNDGAIYQKNADGTEALFDNGKYTQMVNVPLYDA
                     FEVK"
     gene            15149..15823
                     /locus_tag="VIG33_10875"
     CDS             15149..15823
                     /locus_tag="VIG33_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271380.1"
                     /translation="MKKLQILLIVGLLVAVGSAYRQLHEIESSSTSNPPSVTTLESAA
                     PASATATAKGAGGKPAFAVGEFVALVKNEAVAMSQLNDHPEEVEKRLKDLAEKMQEAD
                     VPELQATALNTSLNGDQRFLSVYILGESSLPKAQESLETIATAPIPTMSEARLTNQEE
                     IIRSQAVESLRQPESLKRVLAQVNNSFVVDRAQRSLLYREGKVSRAPEQQDQEALSKL
                     IKKTAH"
     gene            complement(15825..16325)
                     /locus_tag="VIG33_10880"
     CDS             complement(15825..16325)
                     /locus_tag="VIG33_10880"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HEY8271381.1"
                     /translation="MSFLGNFTIESLITSELPTSLLKAKVKEIAASKVFFFDCGVVSA
                     LLNQLNLPLGDNKGSLFETFIINEVKSYISYSNQRADIEYWGGAHQGGEVDLIITVDG
                     VKTGVEIKSSKKYSREFSKHLQELIDNKKIHHGIGVYCGPHEHKDQSVRFLNYLAFLK
                     KLMPEK"
     gene            complement(16339..16935)
                     /gene="thpR"
                     /locus_tag="VIG33_10885"
     CDS             complement(16339..16935)
                     /gene="thpR"
                     /locus_tag="VIG33_10885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEY8271382.1"
                     /translation="MRRLFLAIAAKDGLETVAPQVIKKLKIHAEQKDIEVRWVPMDNY
                     HITLVFLGNTYDEKISEIETITERIAKNFAPFNLKIADLGAFPNDFSSRVLWFGVQNS
                     KALRSLQDSLATELKHLRYKAEEQGFSPHLTIGRLRNPHKTKDMTSPFVRKKFAKIQI
                     NEIVLYESVGSAPFQVYKPIKTFKLSGVPASIEDSDFE"
BASE COUNT         4791 a         3420 c         3987 g         4903 t
ORIGIN      
        1 agaaaaatta aagttgtcat tagtgacttg caccttggca agggacgtat tcttgaaaac
       61 ggtggcagca attccctcga agaattttac tattccgaaa aacttgtgga attcattcat
      121 tattattcct ctggcattta tcgtgattat gaagttgagt tgatcatcaa cggggacttt
      181 ctcaattgtt tacaagtgga ttataaaggc cattttctga ccgtgattac agaggctgtg
      241 gcgctagagg ttattaagtc cataattaag ggacacgaaa aagtctttaa agccatggct
      301 gatttcgctg taaagtcagg gaattccata acttatgttg tgggtaatca cgatcaatcc
      361 atgttgtggc cttcctgtcg tgcttatctg aaccaagtgg taggttcgcc cattcgattt
      421 aaaaatatcg tatacttttt cgatggggtt catgttgaac atggtcacat gcatgaagcc
      481 gcaaatcgta tggatcctaa aaaattcttt ttgaagaaag atttgccaga gccgattttg
      541 aatcttcctt ttggttccca tttttttata gaactcgtgc tgaaggttaa gcagagatac
      601 ccgcatgtgg ataaaattcg tccctttgct aaaatggtga aatgggcttt gtttaatgaa
      661 actcgaatgc tggtaggttc cttttattct ttgattgttt atttcggcaa agctttgctg
      721 gtgaaagatc cccgtcgcaa ttggccgttc cggcgtattt tgcaagtgat tttggaaagt
      781 gcgatttttc cggatttgag cgaggccgca cgtcgagttc tagtggatga ccgggtgcat
      841 acggtgattt ttggccatac tcatgtttat caatatcgcc agtggtcgac ggacaaagag
      901 tattttaata cggggacttg gacggaattg acctctttag atattgtttc cctggggaaa
      961 atcacgaagc tgacctatgt gttgattgaa tatcccgaag atggaggacg tccgcgcgga
     1021 cgcttgaaag agtggcgtgg ctatcaccgg attgaagagg atgtcgcagt atcatagatt
     1081 cgaaaataga agttcggccg aaaccgacct tgattgtacc gaaggtcggc ctaagccgac
     1141 cttgatttag gtcgagctag gcaagaggcc agctaaagaa ccctaagtaa aagcatgatt
     1201 gtaatcaaag tctccgaaat ttacaataga aggctatgac gaatcatgat gaggactcaa
     1261 aagacgtatc agaaaaaacc agcgttgtaa gctcagacac tttcaaaggt cgtattaagg
     1321 ctgctgacaa tgcacctccc gtagtagtag tccttattgg acctccggga tacgtaggta
     1381 agcagtatcc gatttcttcc aatgacatcg tcattggtcg ttcagtggaa actcaagtgt
     1441 atattgacga taaaagtttg agtcgctcgc atgccaagtt tgccgtcgtt gcgggagaag
     1501 tttctattat tgatttgggc tccactaata gaacgattgt gaatggcaat gctttgccgc
     1561 cgctagctcc atgtaagttg aagaacaatg atcaaataaa aaccggcaat gtcattttca
     1621 aatttttaga aaaaggcaat atcgaggccc tgaccaatca gactatgtat gagaaagcca
     1681 atagagacgc gctgacgggg gctcatagta aaggctcgtt gttggagaag ggtcctgagg
     1741 cagtaaaacg ttcagaactt cttagtgaag agatgagtgt tattattttc gatatagatc
     1801 actttaaaaa gatcaatgat acttacgggc atcctgccgg cgactttgtt ttgaaagagc
     1861 tggccaatat agtgagttcg aaattgattc gttcgaatga ttactttgct cgttatggtg
     1921 gcgaagagtt cgttttgatt ctgtcaggtg gcacggcgaa aatggccagt gaaatcgcgg
     1981 agcgcattcg tcagaccatg gaaaaccatg ctttcgttta cgaaggcaag acgattccgg
     2041 tgactatttc cctaggtgtt gtgacaaaaa gcacgaacga cacggattgg cctgaaattt
     2101 ttgatcgtgc cgacaaagcc atgtacgctt caaagcaagc aggccgcaac cgcgtcaccg
     2161 tcgcccagta ggtacgccgt acctttttcg gatgcggcgt ttaatattcg aggtggggga
     2221 tgaaaagttt cttagtagtc gtattaggtg ttgctttatt ctccgtttgc tcttgggccc
     2281 aagagggcaa tgatcaacgt acggtcagtg aatgtgcttc ggaacttaag attaaagttt
     2341 ggtgggatag ccataagtat aaagctaaaa agtactgtga agagtattcc caagctagca
     2401 tcaactgtgc catcaatctc atgcaagcaa agcaattaac ttataccaat ttcgaaaaag
     2461 ctctcaagga ttgcgcacgc actcgaaaat aggttagtaa aatcaaaatg tatttcccat
     2521 gtgaccctgg gcctaagtct gagtgcaaaa agtctctact atttcgaatt ttttgcaccc
     2581 ttcagtatta gaccttgggc agtagttacc attctttttg atgtatattt tcatcttatc
     2641 ttttcattat tttgtcgaag tgcgcggatc tactcttgaa tttttatgca taagaaactc
     2701 ttatgagaga cccaaaatgt tcttaaaagg taaataatat catatattta gagtcattgc
     2761 gactccaaag atgtcggcat ctgtcttgca taaagtataa gcaaggggtc atcaagggga
     2821 ggcattccca ttaacctaaa ctcctggaaa gtgagtctat gctatgttgc gagatctttt
     2881 gaaaacagat cttgagctgg tggaggaagt gaaaactgga aacaggcgat ctttttctga
     2941 acttgtgaag cgacatcaga aaggacttct gagaatgagt ctgagattcg taaaggatct
     3001 cgatgcggcg gaggatgtag tgcaggaaag cttcatcaaa gcctacgaaa agcttcacag
     3061 cttcgaaggc agagcgagtt ttaaaagctg gctcttccag attgctgtga ataatgcacg
     3121 caaccgaatt cgcgactata gaaaaaatat ggtcgatatc gaaaacgtgc agctcgcggt
     3181 aggggctgaa gtggaaggtg gtatggttca cgatgccgtc gccggagtgt tacgagaaga
     3241 ggttgataag cttccggaca gacaaagaac agctttggta cttcgggttt atgaagacct
     3301 tagttttaaa gaaatcgcgg aaattatggg ttgcccttat gacactgcaa aagcgaacta
     3361 tcgtcatgct ttgatgaaac ttcgcgaaat ttttgaagaa caacaggacc tgaaatcttg
     3421 gactgaggaa gtcggaggtt ttctaggtga aaccagccaa tacgtggagg ccgaaggatg
     3481 aaaaagaaac aacttctagg aagcgtactt gatgaggtgc aacttcctca taacaaaacc
     3541 tattacgatc gaatgcatga caagatcatg gctcgtatcg aagatacaga aatggaggct
     3601 cccattcgcc ctgcacggcg cagtttcgaa aaatcgaaac agcttcttcg cgagcaatgg
     3661 agacattggc ttgcaccaca tgaaccttaa agctcaacac ggaaaaagct cgttttcatc
     3721 aacgggcttt tttttgtgac gaaattatcc caagggtacg ccgcgaagtc gcattgattt
     3781 tacgtttcac gttccacata ctttttactc ctgataaaaa cttcgacaat gaagtcgaag
     3841 taagattcaa aggagataaa aatgaaatca ttcatcgtgg ctctcgcaac tcttggttat
     3901 gtaatttccg caaatgcagc tagcgcagac agtttaaaag gtactggggt gtatggtgat
     3961 gcgggttgtg gcctaggttc catggttttc ggaaataaag agggtatcgt tcaggttgtc
     4021 gcggcgactt taaacggcac tggcatgcaa acttttggca tcacttctgg aacttcaaac
     4081 tgcggtcctg gactattttc ttcagccgaa atcaattcgt tcatgcaatc caatgctgtt
     4141 gcgcttgaga acgacattgc tcgaggtcaa ggcgagacct tgagcacgtt aaataatatg
     4201 ttaggttgca gttcgcagtt tggcgatact cttcaaaaaa attacaagaa tatttattcc
     4261 cctgggataa atcccgctca gaaaattgtt gctatggctg aatcttgtca gggttaagag
     4321 tgtttcaaat gacaaaaaaa ggcctcacgg tgatggggct ttctcttttt ttactgattt
     4381 ttttttcaga gcaaaaagcg atggcgtcag aggtcctcga tcgtgctcag cgtgagcatt
     4441 ggtttgaaga tcgtcagtgg ctacgcttag tccaatatca ccctcatttt tttggcggtt
     4501 ataaaagctc tggagaatct gataaattct ttttagcccc taaagggaag agccgtcctg
     4561 atcttgaatt gacggcgaca ctgcttgctc ttcaagagaa taccagaacc ttgcgctcgc
     4621 aagacggacg ttttgaagat cctgtgggct gcgtgtttcc agcgaggaag ctctggcttg
     4681 aaaaaaaagc aggaataaaa tttcccaatc cgcagtgtga acgttttaac cgctttaagg
     4741 aaatccttca gccgcagtcg ttgacttatg tgttttcctc ttattattta aataatccag
     4801 catcggcctt tggtcatact ttcttgcgag tgaataaagc cccctctgct ataaatggcg
     4861 aacgctttga acttgccgat tatggcgttg gctatgccgc tgtgaagact tcggagaatc
     4921 cttttgttta ttcttttcta ggagtttcag gcttgatgcc gggagctttc gatataaatc
     4981 cttattacta caaagtgcgt gagtacaacg atttcgaatc tcgggattta tgggaatatg
     5041 atctcacctt tacgccggaa gaagttgaga tggtgatcgc ctatatctgg gagcttctcg
     5101 acgtgaattt taattatcat tattttactg aaaattgctc ctatcgaatc ctctcggtcc
     5161 ttgaagtcgc tcgaccgagt cttgagctga cggatcatct aaaatcacag gtgatgccag
     5221 ccgatacggt tcagactctt tttcagcaac aaggtttagt tggaagaatc cattttcgcc
     5281 cttcaatccg tgccactttc gagcaacgct atcgtggctt gagtgatgaa gggaaaagtc
     5341 gactcagagg ttttgcccga aatgagtcta tcgctgaaat gatacaggat ctcgaacctg
     5401 tagaaaagcg agggagtctt gatacggcta tggactattt agattatcgg tattcaaaag
     5461 agattttaag aaaagaggaa aaatatattt tcaaaaagga cgttttgagg gcgcgagcgg
     5521 agttaggtgg agcttcagcg cttttaaagc tgtcaaccgc tgagagcgaa gctccccatg
     5581 aagctcaggg atcgcggcgt tggggttttg gttatcgaga atggaataaa agaaatttct
     5641 atttactaga gatgaaagca gcattgcatg atctattaga tccaaaatta ggttatcctc
     5701 ccaccgctga aatcaccatg ggatatttgg gcttgtcatt ggattcaact tctcaagaac
     5761 tggccttgga ccgagcgatt ttgtatgaag tcgtttcctt gtctccgctg aacgacttca
     5821 atcgcgatgt ttcgtggcgc ttgaagttct cattggaaag aggttttgaa aacaattgcc
     5881 tcggtctttg tcgctggacg gaacttagcg gaggttcggg tcttacgacg acagtgtggt
     5941 cggatttgga tctcagtgtc tggctgcgtc tgaccaccca aaccagtact gatttccagg
     6001 gtgatacctg gcgaataggg gcgggtcccg gtctctcagc aagatggaat cgaaatgcct
     6061 gggcggtgat ggcggagatc tatgatcgct acgattacaa aggattgaat ccggagtttc
     6121 ggcaaagctc tcttgggatc aattggagct tgaataaaaa tctcagtctc cgcttcagag
     6181 gagccgaaga gaattccctt cggcttatgg aagcgcgctt gctttattat tattaaaaga
     6241 ttaatttttt acggcggatg aatatgacta acgtgactgt attttggttt cgacgggacc
     6301 tgcgtcttga tgacaacgcc ggtctttttc atgctttaaa agaaaaagaa gaaaaggtcc
     6361 ttccgttttt tatatttgat acagatattc ttgcaggttt ggataaagcc gacgcaaggg
     6421 tgacctttat ttatcaaagg gtcgcggagc ttaaggagcg gcttcagcat ttgaaaagcg
     6481 atctccttat tcgttcaggc agacccctcg aagtttttaa aaatcttcat aaagaaaaac
     6541 atatttcagc tatttattta aaccatgatt atgaaccttc agccataaaa agggacgaag
     6601 aaataaggca atgggcccag acccaagaga ttgaatttaa atcttttaaa gatcagtgtc
     6661 tttttgaaaa agatgagatt gtcaccgata ctggcaagcc ctatagagtt ttcactccct
     6721 acaaaaataa agttctttct catctcccta aacatgtttt tcaattttac gataatgaat
     6781 ccgtgagcag cgcctatgtg aaagtgcaag aggtcgagag agtgttaact ctcaaagacc
     6841 tgggctttgc gatgtcctct ctccattttc caagtaaaga gatcccgctt tctgttatta
     6901 aaaattacgc caaaaccagg gattttccgg ccaatgataa tggaacaacc catcttggta
     6961 ttcatttgcg ttttggaacc atcagcattc gaaaattagc cttcctcgcg cgaaaggagt
     7021 cgagtgtttt tttaagtgag ctgatttggc gggatttctt tatgcagatc ctttggcatt
     7081 atccacaagt cgagaaacga agttataaaa gcgcttatga taaaattgcc tggagaacgt
     7141 ccaaaaaaga ttttgagcgc tgggctcacg gcatgactgg atatccctta gtggacgcgg
     7201 gcatgcgcga attgcaagaa acaggacata tgcacaaccg agtcagaatg gtggccgcga
     7261 gcttcctctg caagcatttg ttaatacatt ggagcgaggg agaaaaatat tttgccaaaa
     7321 agcttttgga ttttgatttg tccgctaata acggcaactg gcaatgggtg gcagggtcag
     7381 gttgtgatgc ggccccctat ttcagaattt ttaacccgga tgctcaggca aagaaattcg
     7441 accctcaaaa ggaatacata aaaaagtgga ttcctgaatt caacacctct cgctatcctg
     7501 ctcctatgat tcagcatgaa gaggctcgag gccggtgttt acaagcctac gcgtcagtta
     7561 attcttacaa ggaatttatt ccttagattt tgcgagggct ttttcaaatt catctttcac
     7621 gatatttatt ttagaagtga tagagtggcc gcaagtgcca ccttcactgc aaactttagc
     7681 aattttacag gtattcccgt cattccagac ccaatcgcca tttccttcag aaataatacc
     7741 tgtaatttta cccgtgctca catcaaacac aggagaacct gaatttccgc caaaaatgtc
     7801 tagattggaa taataaattt tctctaaatt ttttgagatg acacctgagt ccgtgtattt
     7861 cagaggagtt gatgagggat aacccaagac gaaaagagcc gttcccacgg gagtatcaga
     7921 agtaaaatct aaaggaagag catcccgccc tttaacttct ctatcaagtt gaataatagc
     7981 gaagtcaatt ccatctaaga attcgaattt ttgggcaagg atccttttac aagtatagac
     8041 attttcagct ggaactctgg tgtaagaggt atctccagcg gattttaatt tgaaatcaaa
     8101 ggcccagtag taattggcgc aatcattggc atccttaaca cagtggccag ccgttgctac
     8161 gacgttggga gctattaaga aacctgtgca tctagggcct aaaaattctt caccaaatcg
     8221 cacgcttgaa caatgtcttt ttcctaaagt tgtggattct acagaatagc ctccgccttt
     8281 attcatcaga acatctttgc tgaactggcc tggaaccgag cgacctaaag cttgtatggt
     8341 cgatggagct gtgtaaatat tgtttctatt gtcttcgccg tagatggtgt aaggaacgaa
     8401 gtccattgcc agagcctttg tggcgctcac ggtaagaatc attaaggata ataattttga
     8461 taaaatctta agactggaaa actgagtttt cataaacctc acctcattct tgttggcaac
     8521 ttgatcacac agggccaacg gtattgtcaa ggcatagtgg ttggcataat ggtcggtata
     8581 atgtcttagg tcaggaaacc cggtctttta gcgcctctac gggatttttt tctttttcga
     8641 atgctttgca ttcttcgagg aactttttaa aatccccccc tgttttctca tagagttttt
     8701 caaagtcccc gagatcttgc atataagtgc gatagactaa taaacgcgcg ttattaagtt
     8761 tgatgttctg aaatttctta taggattccg ttttcaactg cggcagcatt tcttttttaa
     8821 aatcctcttg cagttgaacg aattgttgct ggcgtttgtt atccgtttgc tcatgagcgg
     8881 gaacattttg ataccacttc tccagttttt ttagctttgt actgagccat gccgagaata
     8941 ttttatcgtc cgcattttca tttcgcacgg tgagcagggt cggggactcg gagccttcct
     9001 ttttgagata gaacatctcg gtgcctttat tacccataaa gacggccatg cgttcattga
     9061 aatcagcttc atgttttatg taaagagtgg catgaacggt ttcgtgaata atggtattca
     9121 ctaaatcata gtctttatat tgaagcatgg acgacaagag cggatcctta aaccatccga
     9181 gagtggagta ggccgaaact ccacgcatgt atgtgtccag gttttcttgt tttagatttt
     9241 cttcttcttt ttttgcatcg agttcattaa agaaaccttt ataaggcatc gcgccgacaa
     9301 tgggataatg ccatttgtag gcttccaact tccatttcgg agcggcactg accacataag
     9361 taacatacgg acgatcgagt aagacaaagc tcgtataatt tttcgtcgct ttaagactta
     9421 aggactgttc agcaaattcc cgagcttctt gcgcaagctg aattttcctt ttttgttcag
     9481 gggtgagttt tggatttttt aaagcctcat cgagagtctc ttgcgagttc atgatcgaga
     9541 gctgattata ggcggatttt acaagatagc tgacttgaca cgaggacaag agaagggcgc
     9601 tgctgatcgt gacagtggcc tttagaacat ccatgtaaca cccgcggaga ccgcatcatc
     9661 attggtcttc gcgccgcctc caatgggagt ctgccaaatg ctatagctag ctttaaagcc
     9721 aaaactttct gtaagagcga tcttgagtcc caaaccataa cttggagcta tggctacgtc
     9781 cggctctaaa atttgcgtgg ggaagcttcc atctttaatt tcctgacgtc tggaaatttg
     9841 cgctgctcca cctttaatat agggctgaaa tagggccttt ttgtcagcaa agacgacgat
     9901 aagatccgtc cctataattt gagttttttg ataaattgta cggggagtgt ccaaggagga
     9961 agcttgctgt ttttgaattc cgacagcttc ggtgtagctc agctccagcg cgagcatttc
    10021 agcaaaataa aaagaaaccg aacctgtggt tgattccgaa tcatagttat tgtttgcatc
    10081 aaaagtcgtt tttttacgac cataagtcag acccatttcc gtaaacatcg catgactgtt
    10141 taacggaagg atgagggaaa tcaggatggt tagattttga aaagtctttt tcatgctttg
    10201 aggatatcaa agcttagagt gaaaaatgag aggtttcacc cctggactgg agcaaagtct
    10261 tattccgaat agaccacggc ccccggattg aagctgacgt taaaactata tttcagatga
    10321 ataaacccat catcctcgac catgtcctga ggtggattgg gaaacatgcc agcctcttta
    10381 aatgccagga ccgccgcttg atcaaagtga ttgataccgg attctttaag aatgtgagcc
    10441 gagtgatagc ggccatcctt agtgagccaa atatctactg aagtcaccca gtttctgcgt
    10501 cccaccacgc ctaagacata gtttcgatca aaagagtcga ctgctttacg cacgcggctt
    10561 tcccagcgaa aacgaactaa ttcttcaata cgcgcgtaga aggaataata aacataacga
    10621 tccgtattga gtgcggtgaa gttcccgatt gaaacatctt gaggcaaggc ttcgccgacg
    10681 gtcgctggag cgttttccgc actttgagaa tactctttta aatttttcgc gatatcggcc
    10741 ccttcaggat cgtatttttt ctctttagtt tctgattttt ctttttggcc cgtagtgcga
    10801 tttttagtca ttccggaaac ggctgcctgc atttctttac gaacacgttg tttttcggcg
    10861 gaaagaaatc gagcgagatc agtatcatcg tccttcaggt cctgtttagg ggctaaagcc
    10921 tggcggacga cttggcgttc tttgttagca attttatttt tattggattg ctctctaggt
    10981 ctttccaaca aatcaatggt ggtgacggag aggggttttg gctcaaaata aggcgcccct
    11041 ttatccagga caaggactaa tacaaagtgg accacaaggc tgaggagaag ggctcgcaat
    11101 aaattcatct tgtctcacta tagaacgaac ctagacaaaa atccaactgg atgcctaaag
    11161 ttgaaacaaa taataccgaa aagagggagg tgtcatgggg gtataatggc aaaggtaatt
    11221 aatatcacaa gtcgcgtaaa gtctcagaat tcgttgatga ataataataa gtctttcgag
    11281 gcgccagtct tagatatgac ggaagcaaga gaagaaatcc tgaataaaga acgtcgcgaa
    11341 gtgaagcgta cgatattaac ggaatttata ggaacttatg cggttttgcc agagcggggt
    11401 cttatgaaag tgactctcta tgatatctcg gaaaatggaa tcgcttttga tcttgaacct
    11461 caagaaggca tgtttcgaga cggtgaagaa gtcgccatca gactctattt aaatcacacg
    11521 acttattttc ctttcaacgt gaacgttatc aaatctcgtc caatggaaga ggaaggggtg
    11581 attcgtcatg gagctcagtt tataaaaggc accgtgaacg atgttgcgct tcatcatttt
    11641 gtgaaattta tcgagaacgt cagtgccggt cttcgtaagg atgagggcga tattctggtt
    11701 tcgaatattt cgtagtttta ggactctgtt ttaatccagc ttcttcaaag cttttcgtgg
    11761 acattagtca ggccttattg tcgaatagac ttaaggcttg tttcattttc ggaacggacc
    11821 atccacggag ggtaaaagtt gagcaaatcc aaacgcagaa aaattgtcgt tgataccaat
    11881 gtcattctgt ttgatgccca agcgattctt cgctttggtg aagccgatgt gcatattcca
    11941 atatccgtca ttgaggaaat cgataagttt aagcgagatc agggcgaaaa tggacgtaat
    12001 gcccgtcaat tcagtcgttt tgtggacgta ctcagggcga aaggttctct tgctcaaggc
    12061 gtgcaactcg acaatagcga gaccatggtc tatatcagca ctgatttgat gttggcggga
    12121 atgccaccag aatttgatca aatcaaagca gacaatagaa tcttaaacac ggccttggct
    12181 ttacaaaaac aatatccacg actcaaagtg gagttgatca caaaagatat taatcttcgg
    12241 attaaggccg atgtttacgg gatctttgca aaagattatg aaccgacgga cgtctctcaa
    12301 gaagatatgt atgaaggtta tcttgaactg gagatttctc cagagcaaat cgatcatttt
    12361 tacaaagaca aaaagcatgt tcatacggga actcaaaagt tatatgccaa tcaatacgtg
    12421 atcatgaagg attcttctaa tccgaatcat tcggcgatcg gtagattttc tcatgcggag
    12481 tccgccatcg tgcctttgat caatccgcct gacagtattt ggggaattca cgcccgcaac
    12541 gtcgaacaag ctttcgcgtt agatagtttg cttaatgatg aaatcctttt tgtttcctta
    12601 gtaggcaaag cgggaacagg taaaactttg cttgccttgg cggcagggct tttcaaaact
    12661 ttggatgaag gccgcttcca aaggttactt gtgtctcgtc cgatctttcc gatgggaaaa
    12721 gacattggtt atcttcctgg agacattgag caaaagttga atccttggat gcagcccatc
    12781 ttcgacaatg tggaattttt gatgggttct gataaaaaag cctcaggccg tgctcaggag
    12841 ttgattaacc agggaatgct caatattgaa ccgctgactt atattcgtgg gcgaagtatt
    12901 cccaagcagt acctgatcgt ggatgaagca cagaacttaa ctccgcatga gattaagact
    12961 attgtgactc gcgcgggccg tggtacgaag gtgatcctta ctggtgatgt ttatcaaatc
    13021 gacaaccctt atgtggactc agccaatagt gggctcacct acgcggttga gagatttaaa
    13081 gggcacccca ttgcgggaca tgtgacattg accaagggtg agcgctcaga acttgctgaa
    13141 ttggcagcga atattcttta attgtgctct ttttgcaaca ggcaaaggct tgcctgttgc
    13201 aaagctttgt tcttaattag agccactcaa tagaaaggct catagatatg atgtccatca
    13261 ataaggggag agacagagaa gtggggttta aggccgtttt ggaggcaaaa gtgacagacc
    13321 aaatggtccg tcaatttgct gaaatgtcag gtgattttaa tcctattcat cttgatgatg
    13381 agtacgcaaa gaccacacgt tttggtcgtc gtattgctca tggtatgatc gtcggtgcgc
    13441 tgatttctcg tacactcaat gaaaaattgg gatcaggcgg agtttatctg gcgcagaatc
    13501 taaaatttgt aaatccagtt tttatcgatg acacgataat cattgaaata acggtcacgg
    13561 ggctgcgtaa ggaaaaaggc atagccactc tcgacactat tgcgaaaaaa gaaaatggtg
    13621 agattgtggt caaaggagat gctattatca tgatggccca ataatcctct aactcatggt
    13681 gacgaaaacg ggttgatttc tgccaatgac gcgaaactca agacgctttt tattgccttc
    13741 gtttttagcc agattaaagt cctagggagg ggggggattt ttttatgaaa caactaaaga
    13801 catttctttt gatgggtatg actgttgtgg cggtaagagc ccaagcagag gatctagccg
    13861 ctggtcaagc aaatgcactg ccttatatag atcaagactt tgcttgtatc actcaacaac
    13921 aggctcagaa atacaccaat gatttttcca ttgatattaa ctcttttggt ggactggagc
    13981 tttgcaatag caatgtagat gccaagaagc tattcaacga tttgcaaatt atcgagcaag
    14041 gccagttcgc agctgttaat tccaatatat tagttaaagg ctatattcct gccgatcagt
    14101 actattcttg gatgaaaaat caaactcgcg gtatgaaccg gggcaatgac atcccttacg
    14161 caacggctta caatcgtatg ggttacttta cgatgcagga tggatgggcg aagatttcaa
    14221 ctttgggtcg tgtcggcgtc gtccttcatg aagcacgcca cacagggggg taccgtcact
    14281 atccttgtaa gcagggcccc tacatgggct ctagcttgga cggttgcgac caagatttta
    14341 cgcaaggggg ttctcatgcg atcgagatgg agtattatgc ccgcgtaact gttgcaggcc
    14401 aaaattttca tccagtttat aaagcgatgg cacgtttgat ggctatgggt cgggcgagtt
    14461 ttgtattcaa tacttcaccg attcaaaaac gtgaagccgt gcttgccttg gaagagcagg
    14521 cagggccagt gatgtttgat ggcacacgca aggtacaaag agaaggcact gattctgtgg
    14581 gacagctgaa acgcacgtcc ttcggcgctg ttatattcaa cggtcttcag gcagtggctt
    14641 tggatgttta tgaaatgaca ggctttcgct caaaaacaaa tgatgactat tcatacttca
    14701 aacttttggg catgaaccaa atgggctctt tgagagattt tgaagagtat gatgtgaatc
    14761 aaaagcgtta tgtgacggct gtgactgcct ccaatcagtt ggcaacttac aattttccca
    14821 aaggatcttg gaatagccct gcgacccttc ctttgacagt ggaaagaacg gcaagcactt
    14881 tgccaacggg tgaaaaaggc tattttttga tgaataacgt cggcgatatt tactctgtga
    14941 acgctgataa tcaacaagtt acttccactg gcaaaaagtg ggatgctcaa gttcaaagtg
    15001 cggctttatt tgggggcaaa atcctccttt tgaaaaatga cggtgccatt tatcaaaaga
    15061 atgctgacgg caccgaagcg ctcttcgata acggtaaata cactcaaatg gtgaacgttc
    15121 ctctttacga cgctttcgag gttaagtaat gaagaagttg cagatcctgt taatcgtagg
    15181 cttgttggtg gcggtgggaa gtgcttatag acagcttcat gaaattgaaa gcagtagtac
    15241 ttcgaatcca ccttcagtga cgactttgga gtctgcagcc cccgcatcag cgaccgcgac
    15301 agccaaagga gccggtggaa agccggcctt cgctgtcgga gaattcgtcg ctttggtcaa
    15361 gaacgaggct gtggctatgt ctcaactcaa tgatcatccc gaagaggtag agaaacgcct
    15421 taaagatcta gctgagaaaa tgcaagaagc cgatgttcct gaactgcaag cgacggcatt
    15481 gaatacgtct ttgaatgggg atcaaagatt tttgtctgtg tatattttag gtgagagtag
    15541 tttaccaaag gctcaagaaa gtcttgaaac cattgccacc gcacccattc ccacaatgtc
    15601 tgaagctcgt ttaaccaatc aggaagaaat cattcggagt caagcggtgg agtctcttcg
    15661 ccagccggaa agtcttaaac gtgtattggc tcaagtgaac aatagtttcg ttgtcgatcg
    15721 tgctcagcga agtctgcttt atcgcgaagg caaagtttca agagctcctg aacagcagga
    15781 tcaagaggct ttaagcaaac tgattaagaa gacggctcat tagattattt ctccggcatg
    15841 agctttttca aaaatgcaag gtagtttaaa aaacggacac tctggtcctt gtgctcatga
    15901 ggtccacagt aaacacctat gccatgatga atttttttat tgtcgattaa ttcttgcaga
    15961 tgtttggaaa actctcttga atattttttc gaggacttaa tctcgactcc ggttttaact
    16021 ccatcgactg taataatcaa atcgacttcg cctccctggt gcgccccccc ccagtattca
    16081 atatccgctc gctgatttga ataagagata taggacttaa cttcatttat tataaaggtt
    16141 tcgaacaagg agcccttgtt gtctcccaga ggcaaattga gttgatttaa tagggctgaa
    16201 acaactccac aatcgaaaaa aaatactttt gaagcagcga tttctttgac tttggctttt
    16261 aagaggctcg ttggcagttc agaggtaata agcgactcaa tcgtaaaatt tcctaaaaag
    16321 gacatttata gggaaatttc actcgaaatc ggagtcttct attgaagccg gaaccccaga
    16381 aagcttgaaa gttttaatgg gcttgtaaac ttgaaaaggg gctgagccca cactttcgta
    16441 aagaacaatt tcattgattt gaattttcgc gaatttcttt ctcacaaaag gcgaggtcat
    16501 atcttttgtt ttatgaggat tgcgaaggcg accgatcgta aggtgaggag aaaatccttg
    16561 ctcctcggct ttataacgca aatgtttcaa ctctgtggct aggctgtcct ggagggaacg
    16621 aagagctttt gagttttgca ctccaaacca aagaacgcga gaagaaaaat cgttgggaaa
    16681 agcccctaga tcggcaatct taagattgaa gggcgcgaaa tttttcgcaa ttctttccgt
    16741 gatggtttct atttccgata ttttttcatc ataagtattt ccaagaaata cgagagttat
    16801 gtggtaatta tccataggca cccagcgaac ttcgatgtct ttttgctcag cgtggatttt
    16861 aagtttttta atgacctgcg gagccacggt ttctaacccg tcttttgcgg cgatcgccaa
    16921 aaaaagtctt cgcattatga ataccgaccg ttcttaaaag aagtgacaac attgttatga
    16981 gatcacctta tcacaaaaca ctgactgctg gaaggactct ccgttacgat ggccgtgaga
    17041 aagtattgtg aaaaaatcat gtcatgtttt ctcatgcaaa tcgcgataca cctgaggtat
    17101 g
//
LOCUS       DATBTE010000292         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_312395, whole genome shotgun sequence.
ACCESSION   DATBTE010000292 DATBTE010000000
VERSION     DATBTE010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312395"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VIG33_10890"
     CDS             complement(<1..226)
                     /locus_tag="VIG33_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271383.1"
                     /translation="MEYHIVVADLAMAYKKLCLFAIIRLRTISLGRTLPGSNECNRNV
                     FRSLSRRGFRIPTSVVFGRIVHVYIAKTAQL"
     gene            complement(295..1161)
                     /locus_tag="VIG33_10895"
     CDS             complement(295..1161)
                     /locus_tag="VIG33_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8271384.1"
                     /translation="MSENNINRVKVFLKGDIIFHEGQPSDHFYVLQKGEVEVSIRKNE
                     RRFVIGTLGPGEFFGEMGLLSNEPRSASCRAITPVEVARISNDDFKKLLKNSHPLVGK
                     IINVLLKRLKKSNSLSANKTASTNIFYAVANLLDLNFKAANSDSILAGLFIKQITATL
                     GILPHQAKAILQRFSDFGLIQKIQNKANSSSLQDEEEGRLLLQSDNLLMEAIIIQDQE
                     PDKVSSESFSSELELMEIKEALELYGIDQKKFYGKMCAGDFPDHLFFINKSVLFSLIQ
                     GRGKQFFEEPGA"
BASE COUNT          450 a          325 c          293 g          491 t
ORIGIN      
        1 ggagttgagc tgtcttagcg atgtaaacgt gcacgattcg tccgaaaacc acgctagtgg
       61 gaatacggaa tccgcgacga ctgagacttc gaaaaacgtt gcgattacat tcattggaac
      121 ccggtaaagt acggcctaaa cttattgttc tcaaacgaat tattgcgaat aagcagagct
      181 ttttataggc cattgccaaa tcggcgacga caatgtgata ctccatgtga agatttttaa
      241 cgcttaatta cacgcccaca gtgctctttc tttaattatt atccgattca agatttaggc
      301 cccaggctct tcaaaaaact gtttccctct accttgtatc aagctaaata aaacagattt
      361 attgataaaa aacaaatgat ccggaaagtc gcccgcgcac attttgccgt aaaacttttt
      421 ttgatcgata ccatacaact caagagcttc tttaatctcc attaattcca actccgatga
      481 aaatgactcg gatgaaactt tatcaggctc ttgatcttga attattattg cctccattaa
      541 taaattgtct gattgcaaga gtagccgccc ttcttcctca tcctgcaaag aagaggaatt
      601 cgctttattt tgtatttttt gaatcaaacc gaaatcgcta aatcgctgaa ggattgcttt
      661 tgcctgatga ggaagtatgc ccagagttgc tgtaatttgt ttaataaaca atccggccaa
      721 tattgaatca ctattagctg ctttaaaatt aagatcgagt aaattggcta cggcataaaa
      781 aatattagtc gaggcggttt tatttgctga aaggctgttc gactttttta accgcttcaa
      841 aagtacattt atgattttcc caaccaaagg gtgtgaattt tttaggagtt ttttaaaatc
      901 atcgttgctg atccgggcaa cttctacagg agttattgct ctacaagaag ctgagcgagg
      961 ttcgttggag agtaatccca tctcaccaaa aaactctcca ggccctaatg tccctataac
     1021 gaatcgtctt tcgtttttcc taatggaaac ctcaacttca cctttctgca aaacataaaa
     1081 gtgatcacta ggctggccct catgaaaaat gatgtcccct tttaaaaaca cttttacccg
     1141 attaatatta ttttcactca tttaagactc actaattatg tatgcatttt ccaaatgcct
     1201 taatcgttga ggctcccggc cttactcaat cttaccgtac ttttgttgtt tatccatttt
     1261 cattttcaaa agttcgcaca actcattctg acagcggtgt aaggtcggcg ctgttaaaat
     1321 tggcgctttg gtcatgcatc actttgtgga ttgcttttat tgtgtttttg cgaaaggcta
     1381 aagccgtata gcgtaccatt acgctgtttt ttgcgtttcc agcgatgcag tttaaaaagg
     1441 agcttaaacg aagcgggggt aggacactaa aagcaggtgt tttaagcgaa gcacctgctt
     1501 ttagtagaca acacatggag cgatacgagg atacgaaact ctagcttttc ttagtgagc
//
LOCUS       DATBTE010000293         6808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_995344, whole genome shotgun sequence.
ACCESSION   DATBTE010000293 DATBTE010000000
VERSION     DATBTE010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995344"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VIG33_10900"
     CDS             complement(<1..588)
                     /locus_tag="VIG33_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY8271385.1"
                     /translation="MEKGWNGLPYNTIAEHYSNRFGEKVYKIPVSVVDDCPNRRGLKG
                     MQTCVFCDVWGSAAQAESVTMELRAQIEKYHGHIAKKYKAKSFLIYFQAYTNTFMKLN
                     DLRHNFETALSYDFVKGFVVGTRPDCLSKGVLDLWQEFHQRSFVAVELGVQSFFDDQL
                     EFMRRGHSAQQSIDAIYKIADSTQADLGIHLIFGNP"
     gene            623..1387
                     /locus_tag="VIG33_10905"
     CDS             623..1387
                     /locus_tag="VIG33_10905"
                     /inference="COORDINATES: protein motif:HMM:NF017266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HEY8271386.1"
                     /translation="MNTLQRWSDIQFTVEITGDGSPSLRLPQGESMHHSGGAAEETEL
                     IYGKVIHQCFQAIGTPHFISVGLGLGYVELTVAREALFTQNLNFTLESFELVPELKEG
                     FKLWLQGQDLNIEIQEVYDQVLKYVLLNSEIPPQKLKELLCQKLENQAWVLHGPLNNH
                     SVFKAKAHCLLFDAFSAKTSPDLWTEEFLVEFFNKATARDAIVSTYASRTALKRALKT
                     AEFLVNVREGFKGKRNSTLAFKGMAFKGFFETSLHS"
     gene            complement(1353..2039)
                     /locus_tag="VIG33_10910"
     CDS             complement(1353..2039)
                     /locus_tag="VIG33_10910"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HEY8271387.1"
                     /translation="MEQKRWFLLLDGQVTGPYGDQDIQNRIDNSTDTLIWGRGQTEWL
                     RPEKWQTSLKALAEQKNLEQQNSRQWKMRVDQKELTPMTFDELIENLKEYTSLSSVRI
                     WTEGFDDWKEVFQVRQVADELGVSRRQHLRVPVMGSMKGTAGLGEVDAKIISISEGGI
                     GVNDVKSLQIGEKFSAKLSSPNLFMDITCNMEVVYLGADGYAGLRFLGLPNDAKSIII
                     SYVKKFQKNP"
     gene            2126..2707
                     /locus_tag="VIG33_10915"
     CDS             2126..2707
                     /locus_tag="VIG33_10915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY8271388.1"
                     /translation="MKYVISGTDRADSNSLKVSKYIQTLCKEMGEEIELIDLREIRNE
                     FLTGPHYGKDQPEGLQAEIDKVVKSDGLIVVCPEYNGSMPGILKYYIDHLKFPDCFEY
                     RPVAFVGLGGMFGGMRPVEHLQQVFGYRNAFIYPQRVFLMNVFKVMKEGELSDPLMRD
                     LLKNQAKGFQGFCKALKDAQLDANSFLEQKASK"
     gene            2707..4596
                     /locus_tag="VIG33_10920"
     CDS             2707..4596
                     /locus_tag="VIG33_10920"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEY8271389.1"
                     /translation="MQNPSHIKHSRNIWIMALGALGVVFGDIGTSPLYALRECFAEEH
                     GIPLEPQNILGILSLIFWTIIMAICFKYMAVVMRADNKGEGGILSLMALASSSEEKKS
                     NIRKYIIVTLGLFGAALLYGDGVITPAISVLSAVEGLYLLTPALEPYVIPVTIFILNA
                     FFLVQRFGTGRIGVVFGPILLVWFAVLGGLGLHGILDNLTVLKAMNPLYAIEFFNYHG
                     FHGFVVLGSVVLVITGGEALYADMGHFGREPIRWAWFLVALPALLLNYFGQGALLLSS
                     PEAISNPFYLLAPKWALAPMVILATLATVIASQALVSGIFSITRQAVQMGFCPRLSTV
                     HTSSREIGQIYVPFVNWSLFLGVIWLVLTFKSSSNLAAAYGIAVTGTMVITTLLAYEV
                     AVRRWKWGLGRALLIFGGFLTFDAAFFGANVMKIAHGGWVPVLMAALIYLLMVTWNKG
                     RQILYARLKERSVSIETFCQMILRTPPLRVTGTAIYMAGDPSGIPVPLLHNLKHNKVL
                     HERVAILTIQTKDEPFVPKKERVLIEEIIPNLYRIIVYYGFMETPKMKHILEACRQRE
                     IHFNVSETTFVLGRETILPSTEPGMSLWREKIFAIMSKNAQRPTAFFKIPPNQVIEVG
                     IQVEI"
     gene            complement(4624..5772)
                     /locus_tag="VIG33_10925"
     CDS             complement(4624..5772)
                     /locus_tag="VIG33_10925"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY8271390.1"
                     /translation="MGSNSKLNKALVLSGGGARGAYQVGVLSAIADIAQASGIHEAFD
                     IYSGVSAGAINATFLAAGADDFSATCKDLVDLWSRLTSDQVFYSDISSIGKIGFNWVK
                     SVSVGGLTGATPGQALLDTTPLFNLIKERLPFAKIQNNLDHGILKALAITALDYQSST
                     AITFVQGQKGLPDWERSRRKSEKAQIVAEHVLASSAIPLLFPPVGVDKRFFGDGCVRN
                     TAPCSPSIYLGAKKLLIIGVRRQTPTAYEHRVLQNVKAPTVARVLNVLLNSVLLDGAE
                     LDVERLAKINSLIDQIPQGAQGSLAYKKVDFVWVAPSADIGEIAAQKHMKLPPIIRYL
                     LKGLGSINEASEIVSYLLFDPSFCTQLIEIGYEDGMRQKEEIKKFLES"
     gene            6018..6587
                     /locus_tag="VIG33_10930"
     CDS             6018..6587
                     /locus_tag="VIG33_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271391.1"
                     /translation="MTLMKNTKIYIYIVVAAFAFSACAKRDSEFAARKNAAGASYVND
                     QQAAAADQAAAANGYVTDILKILSPTQASTGALSVTSQIKVNNNVYQIVTTHSQVNTV
                     SSTSAMFDGANFEVGGICGSDLCNPYYLVVNITRNGQPVKQTAMKKYFYYTDQNSNQD
                     LIMSLGPNEFMSIQDVKTTLDQAVVNSGN"
BASE COUNT         1760 a         1481 c         1564 g         2003 t
ORIGIN      
        1 gggattgcca aaaattaaat ggatgcccaa gtccgcttga gtgctatcgg caattttgta
       61 aatggcatcg atggactgct gggcgctgtg accccggcgc atgaattcca actggtcatc
      121 aaaaaaactc tgtacgccga gttccacggc gacaaaagac cgttgatgaa attcctgcca
      181 aagatctaaa acacccttcg ataaacaatc agggcgagtg ccgacaacaa agccttttac
      241 gaaatcataa gagagagcgg tttcaaagtt atggcgaagg tcattgagct tcatgaatgt
      301 attggtataa gcctgaaaat aaatgagaaa acttttagct ttatattttt tagcgatgtg
      361 tccatggtat ttctcgatct gggcacgaag ctccatggtg acggactccg cttgggctgc
      421 ggaaccccag acgtcgcaaa agacacaggt ttgcatgccc tttaaacccc ggcgattcgg
      481 gcaatcgtca acgacactca cgggaatttt atagactttt tccccaaagc gatttgagta
      541 gtgctcagca atagtgttat aaggaagtcc gttccagccc ttttccatgg gacctttata
      601 gggagctaaa gaggaaaaat caatgaatac actccagcgt tggtccgaca ttcaatttac
      661 agtagaaatt acgggggatg gcagccccag tttgcgcttg cctcaaggag agtccatgca
      721 ccactcgggg ggtgctgccg aggaaacgga gctgatttat ggaaaagtga tccatcagtg
      781 cttccaagcc attggaactc cccattttat ctcagtgggg ctaggcctcg gctatgtaga
      841 gttgacggtg gctcgtgaag ccctctttac ccagaacttg aacttcactt tagaaagttt
      901 tgagctcgtt cctgagttaa aagaaggttt taaactttgg ctccaggggc aagatctaaa
      961 tattgaaatt caagaggtct atgatcaagt cttaaaatac gttctgctga actctgagat
     1021 ccccccgcag aagctcaaag agcttctttg tcagaagctt gaaaatcaag cctgggtgct
     1081 ccacgggccc cttaacaatc actctgtgtt taaggcaaag gcccactgtc ttctgtttga
     1141 tgcctttagt gctaaaactt cgccagatct ttggactgag gagtttttag tggaattctt
     1201 taataaagcc actgccaggg atgcgatagt ttctacctat gccagtcgta ccgctttaaa
     1261 aagagcctta aaaaccgcag aatttctggt gaatgttcgg gaagggttta aagggaaacg
     1321 caattcaacc ttggctttta aaggcatggc ctttaagggt ttttttgaaa cttctttaca
     1381 tagctgatga tgatggattt agcgtcgttg ggaagcccta aaaagcgcaa gccggcatag
     1441 ccatcagcgc cgagatagac cacttccata ttgcaagtga tatccataaa gagattggga
     1501 ctgctgagtt ttgccgagaa tttctcgccg atttgtagac tcttgacatc attgacgcca
     1561 atgcctcctt cgctgataga gataatcttt gcatcgactt ctccaagtcc agcagtgccc
     1621 ttcatgcttc ccatcaccgg gacccgaagg tgttgtcggc ggctgacacc cagttcatcc
     1681 gcaacttgtc ggacctggaa aacttctttc caatcatcaa atccctcagt ccaaattcga
     1741 acagaggaga gactcgtata ttctttgagg ttctcaatca gttcatcaaa agtcatggga
     1801 gtgagttctt tttgatccac ccgcattttc cattgccgag agttctgttg ctctaggttc
     1861 ttttgttcag cgagggcttt caaggaggtt tgccattttt cgggcctaag ccattccgtt
     1921 tgcccgcgac cccaaataag agtatccgtg gagttgtcaa tacgattttg gatgtcttgg
     1981 tcaccgtaag gccccgtgac ctgaccatct aatagtaaaa accatctttt ttgttccaac
     2041 agctgctccc gcctcttaag atccttcttg attattagaa ctaacacagg gattattgtc
     2101 aaatcagctg aaggatataa caagtatgaa atatgttatt tcagggaccg atcgtgccga
     2161 ttcaaattct ttaaaagttt cgaagtacat ccaaactctt tgcaaagaga tgggggaaga
     2221 aattgaactt attgatttgc gtgagattcg caatgaattt ttaacgggtc cgcattatgg
     2281 aaaagatcag cctgaagggc ttcaggcgga aattgacaag gttgtaaaat ccgatggtct
     2341 catcgtcgtt tgcccggagt acaatggctc aatgccaggt attttgaaat actatattga
     2401 ccatctgaaa tttccggact gttttgagta tcgtcctgtg gcctttgtcg gcctaggagg
     2461 tatgtttggg ggaatgagac ccgttgagca tttgcaacaa gtctttggct atcgcaatgc
     2521 tttcatttat cctcaaagag tttttcttat gaatgtattt aaagtcatga aagaggggga
     2581 attgtcggac ccgctcatgc gggatctttt aaagaaccag gccaaaggct ttcaaggttt
     2641 ctgcaaagct ttaaaagacg ctcaactcga tgccaatagt tttcttgaac aaaaagcttc
     2701 caaataatgc aaaatccatc tcatattaaa cattctcgca atatttggat aatggccttg
     2761 ggcgcgctgg gcgtggtatt tggagacatt ggaacaagcc ctctttatgc ccttcgagaa
     2821 tgttttgccg aggaacatgg aattcctctt gagcctcaga atatcctcgg tattttatca
     2881 ttgatttttt ggaccatcat catggctatt tgctttaagt acatggcagt ggtgatgagg
     2941 gctgacaata aaggcgaggg ggggattctc tctctgatgg cgttggcttc aagcagtgaa
     3001 gaaaaaaaat ctaatattcg aaaatacatt atcgttactt tgggactttt cggcgctgca
     3061 cttctttacg gagatggtgt gatcactccg gccatttccg ttttgtccgc ggtggaaggc
     3121 ctttatcttt tgactccggc gttggaacct tatgttattc ccgttactat ttttatttta
     3181 aatgcctttt tcctcgtgca aagattcgga actgggagga ttggggtggt gtttggtccc
     3241 atactgttag tgtggtttgc agtgttaggt ggcttaggac ttcatggcat tttagataat
     3301 ttgacggtgc ttaaggcgat gaatcccttg tacgcaattg aattttttaa ttatcatggg
     3361 tttcatggtt ttgtagtttt gggttcggtg gtgttggtta tcaccggagg cgaagctctc
     3421 tatgcggata tggggcattt tggacgagag ccgatccgct gggcttggtt tttagtggct
     3481 ttgcctgcgc tcctcttaaa ctattttggc cagggagctt tattattatc tagtcctgaa
     3541 gcgatttcca acccctttta tctcttagct ccgaagtggg ccctggcgcc gatggtcatt
     3601 ctggccacct tggccacggt gatcgcctct caagctttgg tttcaggaat tttctccatt
     3661 actcgtcagg ccgtgcagat gggcttttgt cctcgcctct cgacggtcca cacttcgagt
     3721 cgtgaaattg gacagatcta tgtgcctttc gtaaactggt ctttgttttt aggagtgatt
     3781 tggctggtgc taacctttaa atcttccagc aacttagcgg cggcttatgg gatcgccgtc
     3841 accggaacca tggtgattac aaccttgttg gcttacgaag tggccgtgcg cagatggaag
     3901 tggggactgg gtagagcttt gcttattttt ggtggatttc tgacttttga tgcggccttc
     3961 tttggagcga atgtgatgaa gatagcccat ggtggatggg ttcctgtgtt aatggccgct
     4021 ttgatttatc ttttgatggt cacctggaat aaaggacgtc agattttata cgctcgcctg
     4081 aaggagcgct ccgtttccat tgaaaccttt tgtcagatga ttcttcgtac gccccctctc
     4141 cgggtcacgg ggaccgcgat ttatatggct ggagaccctt cgggaattcc tgtgcccttg
     4201 ctgcacaact taaaacacaa taaggttctt catgagcggg tggccattct gacgattcaa
     4261 accaaagatg aaccatttgt tcccaaaaaa gaacgggtgc ttattgaaga aattatccct
     4321 aatctttacc gtatcatcgt ttattacggt tttatggaaa ccccaaagat gaagcacatt
     4381 ctcgaggcct gccgtcagcg cgaaattcat tttaatgtgt cagagaccac ctttgtgcta
     4441 ggccgtgaga ccattttacc cagcacggag ccgggaatga gtctttggcg tgaaaaaatc
     4501 tttgcgatta tgtccaagaa tgcccaacgt ccaacagcct tctttaagat tccacccaat
     4561 caagtgattg aagtgggaat ccaagtggaa atctaaactg ggatgctaaa ttaagatttt
     4621 aatctaagat tctaggaatt ttttgatttc ctctttttgc ctcatgccat cttcgtagcc
     4681 gatttcaata agctgagtac aaaacgaagg gtcaaataaa agataactga cgatttcact
     4741 tgcttcattg atgctgccaa gtccttttaa taaataacga atgatcggtg gcagcttcat
     4801 gtgtttttgt gctgcaatct cgccaatgtc ggcagagggt gccacccata cgaaatctac
     4861 ttttttataa gctaagcttc cttgagctcc ctgagggatc tgatcaatca aagaattgat
     4921 tttagctaac ctttcaacat ccaattccgc tccatccagc aggactgagt tcaggagaac
     4981 attgagaacc cgggcgacag tgggcgcttt tacgttttgc agaacacgat gttcataggc
     5041 ggtgggagtc tgacgtcgga ctccaataat caaaagcttt ttggctccaa ggtaaatgct
     5101 aggactgcaa ggagccgtgt ttctcacaca accatcgcca aaaaatcttt tatccacacc
     5161 cacaggagga aataaaagag gaatcgcgga ggaggctaag acatgttccg cgactatttg
     5221 agctttttca ctcttacgcc gcgatcgctc ccagtctgga agaccttttt gaccttgaac
     5281 aaaggtaatt gccgtggagc tttgataatc cagggctgtg atggccagtg cttttaaaat
     5341 cccatgatcg aggttgtttt gaattttagc aaaaggcaaa cgttccttga tcagattgaa
     5401 caagggagtg gtatccaaga gcgcttgtcc aggcgtcgct ccggtaagcc cccctacaga
     5461 aacagattta acccaattaa accctatttt accaatggag gaaatatcag agtaaaagac
     5521 ttggtcactg gtcagtcgac tccaaagatc tactagatcc ttacaagtgg cgctaaaatc
     5581 atcggctccg gcggctaaga aagtggcgtt aatagccccg gcgctgaccc cagaataaat
     5641 gtcaaaagct tcgtgaatgc ccgaggcctg cgcaatatca gcaatagccg acaggacacc
     5701 cacttggtaa gctccgcgag ctccacctcc tgaaagaact aaagctttat ttaacttaga
     5761 gttagatccc atagtgttca gcttaactgg taatcttaaa atgagacaac ggcttttact
     5821 atgtccagac tctaaacgag agtgggccta aaacgtccct ttgtgccaga ctcgatggag
     5881 agattttatc tctatcaaag actctttgtt tttgctattt aaaatcagat acttagaaaa
     5941 gggattcggg gagtccctgt ggtttgtctt ttgctttcta aagagatggg aatgaagttt
     6001 taaactagga ggcttttatg acactcatga aaaacactaa gatctatata tatattgttg
     6061 ttgctgcctt tgccttttct gcctgcgcaa agagggattc tgagtttgca gcgcgaaaaa
     6121 atgcagcggg cgcttcctat gttaatgatc agcaggcggc ggcagcggat caagcggccg
     6181 cagccaatgg ttatgtgaca gatatcttaa aaattttaag tccaacccag gcctccaccg
     6241 gcgccttatc ggtcacttct caaattaaag tgaataacaa tgtttatcaa attgtaacaa
     6301 ctcactccca agtgaatacc gtttcgagca cctctgcgat gtttgacggt gcaaactttg
     6361 aagtcggggg tatttgtgga tctgatcttt gcaaccctta ctacctggtt gtgaatatca
     6421 ctcgcaatgg gcagcccgtc aaacaaacag ccatgaagaa atatttctat tacaccgatc
     6481 aaaactctaa tcaggatttg atcatgtcct tagggcccaa tgagtttatg agtattcaag
     6541 atgtaaaaac gactctggat caagcggtgg ttaattctgg aaactgaatc taagctcctg
     6601 ggcttttttc ttgataattt ctcagatact ttctagtctc ccaactatgc ttgcacaatt
     6661 tattgatatt ttcttacatc tggatgttca tttagcccaa cttgccgcca cttacggaat
     6721 tttgatctat gtgattttat ttcttattat tttcgctgaa acaggcttgg ttgtaacacc
     6781 ttttctgccc ggcgattcct tgctgttt
//
LOCUS       DATBTE010000294         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1336176, whole genome shotgun sequence.
ACCESSION   DATBTE010000294 DATBTE010000000
VERSION     DATBTE010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336176"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..>1581)
                     /locus_tag="VIG33_10935"
     CDS             complement(166..>1581)
                     /locus_tag="VIG33_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HEY8271392.1"
                     /translation="KSPVDEKNIHTFPIQELGPDFFDPVEAASFPAGILRFRNQEAAQ
                     FIGLHPLSEAQWKEHFWSFKPLPGNLPQPLALRYHGHQFTHYNPEIGDGRGFLFAQFP
                     KNKTWYDLGTKGSGRTPYSRDGDGRLTLKGAFREALATELLQSLGVNTSKTFSIFETG
                     ESLVRYDEPSPTRAAVLVRLSHGHIRIGSFQRLAYFKDTTNLQKLTDFCLKYYYPGIS
                     SDSNTADERAFLFFQTVANKCALLSAQWMLAGFVHGVLNTDNINITGESFDYGPYRFL
                     PRYDLRFTAAYFDQQGLYAYGRQPVMMLWNLQRLGESLKLRYHALPVDEILEEFSDEF
                     NVHVKRLFLMRLNLKSNGTEKDAHLLAAFFNFLETSPALFEQCFFDFFGGAASSRIQN
                     SPQKEHYSGNDFDNFITELKSFETAEPSKSLHSYFKNEKPCTLLIEEIEEIWKNISEN
                     DNWTAFEQKIETIRNFRGIYS"
BASE COUNT          416 a          328 c          416 g          421 t
ORIGIN      
        1 aattccctcg aatacgtcag aaaaagtagc agtggtttat catttgacga aatgaatgac
       61 cgtgcaagca cctgcccttg ttgcatacgt tatttttata aaaaaataaa tgtcgttaac
      121 caacttgtta tttgaaatga cttgccggtt ttagtcggca catctttaag agtaaattcc
      181 tcggaagtta cggatcgtct cgatcttttg ctcaaaggcg gtccaattgt cgttttcaga
      241 aatgtttttc cagatttctt cgatttcttc gattaaaaga gtgcagggtt tttcgttctt
      301 gaagtaacta tgcagggatt tacttggctc ggcggtttca aaagacttca gctcggttat
      361 gaagttgtca aaatcattgc cagaataatg ttctttttga ggcgaatttt gaatccgcga
      421 agaagcggct ccgccgaaga aatcaaagaa gcactgttca aacaaagcgg ggctggtctc
      481 aaggaaatta aaaaaagcgg ccaggaggtg tgcatccttc tctgtgccat tggattttaa
      541 attgaggcgc atcaggaaaa gacgctttac atggacattg aattcgtcgc tgaactcttc
      601 gaggatttca tccacgggca aagcgtggta cctgagcttg aggctttcgc ccaaacgctg
      661 cagattccag agcatcatca ctggctgacg tccgtaagcg tagagacctt gctgatcaaa
      721 ataggccgct gtaaaacgca gatcatagcg cggcagaaac cggtaagggc cataatcaaa
      781 gctctctccg gtgatattga tattgtcggt atttaaaacg ccgtgaacaa agcccgcaag
      841 catccattgg gccgagagca gggcacactt atttgccacc gtttgaaaaa ataaaaacgc
      901 acgttcatcg gctgtgttgc tgtcagagga aattcctgga taataatatt taagacaaaa
      961 gtcggtgagc ttttgcagat ttgtcgtgtc tttaaaataa gccaagcgtt gaaagctgcc
     1021 gatgcggatg tggccgtggc tgagtcttac tagaacagcg gctctggtgg gggaaggctc
     1081 atcgtagcgc acgaggcttt ctccggtttc aaagatactg aaagttttgc tggtgttcac
     1141 cccgaggctt tgtaaaagct cggtggcgag ggcttcgcgg aaggcccctt taagggtcag
     1201 gcgtccatcg ccatcgcggg aatagggggt tctgccgctg cctttggtgc ctagatcata
     1261 ccaagtttta tttttaggga actgtgcgaa gagaaagccg cggccatcac cgatttcagg
     1321 gttgtagtga gtgaattgat gaccatggta acgaagggcc aaaggttggg gcagattccc
     1381 gggaagaggt ttaaacgacc agaaatgctc tttccactgg gcttctgaga gtggatgcag
     1441 accaatgaat tgggcggctt cctggttgcg aaaccgcaaa atgcctgcgg gaaagcttgc
     1501 ggcctctacg gggtcaaaaa agtcaggccc gagttcttgg atggggaagg tatgaatatt
     1561 tttttcgtcc acagggcttt t
//
LOCUS       DATBTE010000295        16380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2431051, whole genome shotgun sequence.
ACCESSION   DATBTE010000295 DATBTE010000000
VERSION     DATBTE010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 16380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16380
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2431051"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VIG33_10940"
     CDS             complement(<1..552)
                     /locus_tag="VIG33_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271393.1"
                     /translation="MKDYFFGKVKSAQFVVAALIGIAAQGAVAATTTLTFDDIANGTN
                     LSTEYQGVGVTASGATIIVATSTPWPIANTGLNVAYASSGLMTFNLNSSITGNVQSVS
                     AYISGDTSTGLYAYDAGGSLVGQAVTVGAASNMLLSVTSSGNPIASVAIHDGGSSFTI
                     DTLSFVAAPPVLTCTDVAQQLYNA"
     gene            complement(790..1371)
                     /locus_tag="VIG33_10945"
     CDS             complement(790..1371)
                     /locus_tag="VIG33_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271394.1"
                     /translation="MRCKEFSLLLKMLGIVGMTFALTACSGNNGQSKSARDTGFNPDN
                     GDDSTHKPDPATGKIVEKFKALQFQKPTQEEAEFLNSMELDLTAGDRAFFSFSYAPDE
                     EGTFYFSLAQARLHLQGCKKAPDRSYKMRIFWQQIQGKKRVIVKEFSPNIESFDFRAG
                     NKYILSYALMDLKEFSDCQSATLKFASFLKNYK"
     gene            complement(1414..2937)
                     /locus_tag="VIG33_10950"
     CDS             complement(1414..2937)
                     /locus_tag="VIG33_10950"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEY8271395.1"
                     /translation="MLLKLFLISFLSLLIGMGLGSLNACHSSRKRQEQLRQERIEEQR
                     VLQEKAREAAIKAKKEAEAKLFTQLKSSELRLLVQNAVVYGYPLVLMDVSQQVMTGGI
                     VVNEFRHSRKIPEAGESGAIHPSVDTLASVAWVDLSKEPVVLSVPATGQRYYMMSILD
                     AWTNVFASPGTRTTGNGPGNFALVSPNWQGEIPVNAQRIQAPTSMALILGHTLIKDPQ
                     DYPMVHAIQNRFRLTPLSQFSHIPIPADLRSESDLKGTPVEIVRVMDAQTFLTRLSYL
                     MKENAPSSEDQVMLESLEKIGVTPGHTVDFASLPSEVKSSLDQAVLEGYIRVEELARY
                     IPGRVMNGWVSGLEVGNYGTNYDARAAMARMGLGANLPEDAIFPTARVDSDGLPLTGA
                     QKYILHFAKGQWPPVNAFWSLTLYNERQGFVENPLHRYSLGSKNKLKAMKDGSLNIYI
                     QATNPGRAKLANWLPAPQGEFNLMMRLYWPKQAVLNGEWQVPGVQKVRSRPAKLTLR"
     gene            complement(3050..4591)
                     /gene="hutH"
                     /locus_tag="VIG33_10955"
     CDS             complement(3050..4591)
                     /gene="hutH"
                     /locus_tag="VIG33_10955"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEY8271396.1"
                     /translation="MQTLLLTGENITLENLHEIAHTPEMKAELAPKARAEMQKSRDYI
                     EGRIKNGEVMYGVNTGFGAFSSVRISDSEIEQLQRNLIRSHSMGIGEPFSKVQSRAMM
                     VLRANALARGHSGIRPVVVDKILEFLNNDIIPVIPCQGSVGASGDLAPLSHLALAVIG
                     EGEVWQKGEIVSVKKVLTEKNIEPLELKAKEGLSMINGCQVMTSIGLLAAWEARRLLW
                     MADLAGAMSLEGLRGSRKPFDPLISATRAHPGEAKTARNLLKLMGATSQIGESHLAND
                     QRVQDAYSLRCMPAVHGAAKDALRYIVKVLETEANSSTDNPLVFADDNKVLSCGNFHG
                     MPVAHAMDFAGIAISSQASISECRISKMISPQMSELPAFLTPNGGLNSGHMIVQVAAA
                     SLVSENKVLAHPASVDSIPTSAEKEDHVSMGTIAARKFEKILRNAENVVSMELLSACQ
                     ALDLLAPLKPTAAVQAAYDHIRKQVPFAKEDRYFAKDVAVIREMLTKNEILQSVKAAV
                     GELEW"
     gene            complement(4594..5889)
                     /locus_tag="VIG33_10960"
     CDS             complement(4594..5889)
                     /locus_tag="VIG33_10960"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8271397.1"
                     /translation="MTKFTDLPLVAPLLASLKDAGYETPTPIQMQAIPLILEGHDLLG
                     VAQTGTGKTAAFCLPILQLLAKNHVRREPKCPRCLILTPTRELAIQIHQSLVIYSKHL
                     KMKHAVIFGGVGQGNQVRDLAAGVDVLVATPGRLMDLFGQKHLRLDKVEIFVLDEADR
                     MLDMGFVQDVKRIIPLLSKKRHNLFFSATMPSEVQQLASRILHEPRKVEVTPQATTAE
                     KIEQTVMFVEKSKKLDLLIHLLKDRSLDKVLVFMQMKHGANRIVEKLLKQGFQAAGIH
                     GDKSQGARQRALEEFRSGKIRVLVATDIAARGLDIDGISHVINFELPHQPDSYVHRIG
                     RTARAGAEGKSIAFCTAEEKSYLFAIEKTTRQTVTVMTDHPFHSTESQNAAVTSVGKA
                     KAALEKRQAGYGPKRRSGGGSGNGGGKKRRFGGFNKSKG"
     gene            complement(5949..6632)
                     /gene="rpe"
                     /locus_tag="VIG33_10965"
     CDS             complement(5949..6632)
                     /gene="rpe"
                     /locus_tag="VIG33_10965"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY8271398.1"
                     /translation="MESKVETEFNPKSMTKLVAPSILSADFANLERELKAIATAGADW
                     VHVDVMDGHFVPNLTIGIPVVESLKKVSPLPLDVHLMIEKPERYVEDFIKAGSDYLTI
                     HVEATENPHQVLKKIRSLGAKAGITLRPGTPLRDILPFLSEVDLVLVMTVEPGFGGQS
                     FMMDQIEKMKILREKREQHHYSYLIEVDGGVNEKTLSYCQDADVLVAGSYVFRNDYKK
                     AIDFLKSSK"
     gene            complement(6622..7572)
                     /gene="fmt"
                     /locus_tag="VIG33_10970"
     CDS             complement(6622..7572)
                     /gene="fmt"
                     /locus_tag="VIG33_10970"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEY8271399.1"
                     /translation="MSKVRICFLGTPDFAVTSLKALMDDEHFEVVGVVTQPDRPAGRK
                     MQLTPSPVKSLAQARGLRVISPESLKKNPLILAEIQRWGAEVAVVVAFGQILSQEFLD
                     LFPFGAVNVHGSILPRWRGAAPIQRSLEAGDKESGVALQKVAKKLDAGDIIGIRRLSL
                     DDEINAQELHDKLAVLGADLLSVELMDYMRGNLVPTPQNESQVTYAHKIEKSEGEINW
                     LLPAVEIHNKVRALVMGPGTYTWLNGKKIKIHKTKVSEKSGAPTKTGMVMDVNQEFLA
                     VQCDSGILNIYEVQPESRTRMKVSDFLKGHPLKAGDHFGI"
     gene            complement(7569..8210)
                     /gene="def"
                     /locus_tag="VIG33_10975"
     CDS             complement(7569..8210)
                     /gene="def"
                     /locus_tag="VIG33_10975"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY8271400.1"
                     /translation="MILEILKFPDPRLREVSKQIDKVTAKHTQLAHDMIETMYSARGI
                     GLAAPQVGELVRMLVIDTRPRDKEGRRYEYEEMTELEQAVPQPLVLINPVIVKGEGKT
                     TFDEGCLSLPGYYETVERFEYVEIKALNEKGQEIVVKSDGLLAICMQHEMDHLEGTLF
                     IDHLSFIKGNKIKNQIKKYGYPVKKDEDAGEEETETEVEAVPVKTKKKESVKA"
     gene            complement(8257..9336)
                     /gene="lptG"
                     /locus_tag="VIG33_10980"
     CDS             complement(8257..9336)
                     /gene="lptG"
                     /locus_tag="VIG33_10980"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEY8271401.1"
                     /translation="MNRIDRYVSGLFWGFFIGGSLVFVTIFLAVDAMSTMVSHKELPL
                     SVLMNFYVYYMPEVFNKLLPIACLLGTILAISSMNKANELVALFASGMSLFRITLPLL
                     VWVSVISAGAFVTMDRLGPALARKKNFILYNDIEKKPSLFSVVKTNKIWYRSKNAIFN
                     IKTLSVKGDTAQSLTLYFFSPEWDLMQMITANKVELKDSTWNLIDGSVTLFTGNSSFP
                     LTSQFKTKKIVMGEDAKELQSTGQTSEMLNQAELLRFIERNKEAGLDTIRYEVDYHSK
                     FSFAFAGLVMCLLGIPFSVGKARSGGSMMNIGIVILLVFAYWILFSSFITLGTHGHLN
                     PILAAWAPNLLMATVAYFFMLRLKR"
     gene            9385..10536
                     /locus_tag="VIG33_10985"
     CDS             9385..10536
                     /locus_tag="VIG33_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8271402.1"
                     /translation="MALRVLLADESSTIKRVMQLALQDFGVEVKSVPVGVDVLPISKT
                     FKPDVIFADVLLAKRNGYEVCAELKADPETKHIPVILMWSSFMEVDEPKISAANPDAR
                     LEKPFDAETLRQIVQTLAPKTQSNPISPYLQFPKMPEFNETPVSTQASAKPSLQPLTS
                     PEGAMKKPMGKIPEVAPINFDQFDPTTAEENIFSIPEEDTYSGISHLSHETPEQNLRA
                     VATDEEFASVPLNSSQQKDPLEEDGWQRQDLKKFKIQPPVEATRPALDERADEEDFAK
                     KFVIPQEASDNDSHPEAEGEFEEIQFGEDLTSTRIGPPMQTKASPKVSTKSMLPPGVT
                     TDVEMIERVIRDEAREVIESICWKVIPEIAERIVREEIHKILRDVEKSN"
     gene            10574..11416
                     /gene="nadC"
                     /locus_tag="VIG33_10990"
     CDS             10574..11416
                     /gene="nadC"
                     /locus_tag="VIG33_10990"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY8271403.1"
                     /translation="MTLLELIRAAIKEDMPSGDVTTEALALKPHHGRARLKAKEDIVL
                     SGAMAFEQTMLNLEPNCKVKWHFEEGDPILKNQIICTIEGDLVQVLKAERVALNFLGH
                     LSGIATFTRRFVKALEGTKARVLDTRKTLPGFRDLEKKAVVHGGGLNHRLNLSTAILI
                     KDNHIAIMNGLAAAVSRVRQHSQLPIEVEAGSLEEVKVAIEQKPHRILLDNMNNETLK
                     QARALIPQEIETEASGNMTLERVRSVAEIGIDFISVGALTHSAPCADISLVFDWEDTC
                     KTLP"
     gene            11398..12153
                     /locus_tag="VIG33_10995"
     CDS             11398..12153
                     /locus_tag="VIG33_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthetase"
                     /protein_id="HEY8271404.1"
                     /translation="MQDSPLNDVRLGTITKKWAESNRLFVHYEASMGSTNDRAKGLAF
                     EERLLQEGLCLFLTDDQTAGRGRGKNIWLNSKPGSALLSSWSYLLNMKPQPTTSCLVG
                     LAVYRALSTTWPFLNWNIKAPNDIYIDDKKIAGILLESVIQGDETRLILGLGINVLAS
                     PAEVGTAKSILESLPPGVPLLGQDWTACLDRLMFEMSEAISHCEEPLSPSEQYSLLMA
                     LNAHPLLKEKYKGLEPNGTLLMGSGKKINWWEL"
     gene            12154..12702
                     /locus_tag="VIG33_11000"
     CDS             12154..12702
                     /locus_tag="VIG33_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEY8271405.1"
                     /translation="MALTITPFPEIGLACPDFTLPAVDGKIYSLKDFQKGHPLVVMFI
                     CNHCPYVKAIEDRLIQLGQDLNALHVNILAICSNDEETYPEDSFANLKKRWTEKSYPF
                     PYLHDKTQEVAKAFGALCTPDFFVYDKDLKLAYRGRLDNSWQEASKVTQRELFEAVKL
                     LVHGQPAPQQQTPSMGCSIKWL"
     gene            12704..13255
                     /locus_tag="VIG33_11005"
     CDS             12704..13255
                     /locus_tag="VIG33_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEY8271406.1"
                     /translation="MKLLRSFLIAVLLVVISIGAWLFYYLGAFKPVDIREVQKEPMKM
                     IYKDHTGPYYKIVAVIQEVETWAKAHNVDCSESFGEYIDDANVVEEARLRSHGGCIVK
                     EFPPNLPEGIKTREVPARKYVMAIFNGSPGIGPMKVYPKVESYMHEHHLTMDGAILEI
                     YVIHSEKAMTTTYLFPLADSLPK"
     gene            complement(13260..13985)
                     /locus_tag="VIG33_11010"
     CDS             complement(13260..13985)
                     /locus_tag="VIG33_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271407.1"
                     /translation="MNSKLRDSSGMSILEILICIGLLSVFIVMSLIFSDSLNHSTQLG
                     RTMATRDRISSGVRSIAGMPAALRNAMRAAQVDGVTPFNVNFNNCVAGTQANACVNNQ
                     EYPLALFSPTVAMDAAGSLLGLLQITSAVGVVATNRFDSFGVPCVQTNPDCLFVVHTS
                     FRAQCPPATLSATAPASSDPAYIPLLIPMATCTVAEAVNVFFSVEVDPAALAANPGLS
                     VFSAPITGSVATSVKLIFGNDPW"
     gene            complement(13982..14596)
                     /locus_tag="VIG33_11015"
     CDS             complement(13982..14596)
                     /locus_tag="VIG33_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY8271408.1"
                     /translation="MSSVHNSSNSGFTLVEVLVATGLFGLLTAAVAIAAMAFLKSAQQ
                     TNLRITRDQIVTQLKMTALNKKAILLSIKKPENVALYNCVCGVGTCANVQQPYLAFTL
                     YGTNGNIESPLYYDSSGSPCDPTSPQCVVRVKTSFFAQCKPDLASVNQNPPLTCNGTP
                     AEFVSIVYTVDENPAAVSGQSVRLKPVSGPIYIQTVDMDPGVCL"
     gene            complement(14596..16146)
                     /locus_tag="VIG33_11020"
     CDS             complement(14596..16146)
                     /locus_tag="VIG33_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271409.1"
                     /translation="MYINSNMQNKYKLKSSLGFPNPLHNREGNIVLGILSGVIVIGVF
                     LQTTGFGKMAQGNAFISGARIKESRDAIVYQIERYSSLPAIFRNSLATTDNSALQNCV
                     LGNGSLPCYGDGTEYPVSLYAAFLSNTGAQRLTTAGPVSDNAYQPALYDTRGNLCQTG
                     ATSASLACPFEVFTTFTAKCPAAVGSPCPAAESISIHYVIRIPNNVFSGVKERTILAT
                     IDKFAPNIQTKDILPPAYGYVPNKFTLASLVSVQPGVTTTNPTYIQVRDIVQSKFIGT
                     SQVSNIDNFTDNLFNNFKITDPSLVAAYSDFLLLNPQAAYSSLGAVSYMMAHQVGYTI
                     SAAEVNSISAATSWITDPLLATALGGAGINDLTLAQALVNSLAGITNPILLAALAQYH
                     VTDPAKSQALAASFAAYPVNQDITRGVVSGAWQAGITDSATLKTYLSVIVAAEVTSLS
                     VAFNIITQGVTDIATAAQMQANYVEPTSATTTQTTPTASPGSTPVPVALSPICDPTTT
                     CASTPSSF"
BASE COUNT         4562 a         4070 c         3528 g         4220 t
ORIGIN      
        1 agcattataa agttgctgtg caacgtccgt acaagtcaga accggaggtg ctgccacgaa
       61 agacaaagtg tcaattgtga aactagagcc cccatcatga atagcaacac tggcaatggg
      121 attccccgaa gaagttaccg aaagaagcat attactagct gcgccaactg taaccgcctg
      181 ccccactaaa gagccccctg catcataagc atagagacca gtacttgtat cacctgagat
      241 ataagctgaa acactttgaa cattacctgt aattgaagag ttcaaattga aagtcataag
      301 gccagatgaa gcatatgcaa cattcaaacc cgtattagca ataggccatg gggtactagt
      361 agcaactatt atcgtcgccc cagaagccgt cacccctact ccctgatatt ccgtcgagag
      421 atttgttcca tttgctatat catcaaacgt caacgtcgtc gtcgctgcca ccgcaccctg
      481 tgctgcaatc ccgatcaacg cggccactac aaactgagca ctcttcactt tcccaaagaa
      541 atagtctttc attatacatc ctctttcttc ttagtgtaaa atcgagacaa gttttctgcc
      601 ttcgataaaa taagaatacc cgccgaacga ggatacttaa acaaattttc cttccttgaa
      661 cctcttcaat acgtcgaagc ttttatcaac cgactctagt ttatactatt attcgcagag
      721 tgtcccgaat caacacgttg gcattacaat ccatatccaa cttcgagact gaaattacat
      781 gatcaacaat cacttataat tcttcaaaaa actcgcgaac ttcaaggtcg cgctctgaca
      841 atctgaaaac tccttcaaat ccatcagtgc atagctaagg atatatttgt tccctgctct
      901 gaaatcgaag ctttcgatat ttgggctgaa ctctttcaca atgactcgtt ttttgccttg
      961 gatctgctgc caaaagatgc gcattttgta actgcgatcc ggggcttttt tgcatccctg
     1021 caaatgaagc cgcgcctgag ccaatgaaaa ataaaaagtg ccctcttcat caggcgcata
     1081 tgaaaaacta aaaaaagccc tgtcccctgc ggtcagatca agttccatgc tatttaaaaa
     1141 ctccgcctct tcttgagtgg gcttttgaaa ttgcaaagcc ttgaacttct ctacaatttt
     1201 tccggtggct ggatcaggtt tatgagtaga atcgtcccca ttgtcaggat taaacccggt
     1261 atctcgagct gatttggact gaccgttatt accactgcac gccgtgagcg cgaaagtcat
     1321 ccccactatt ccgagcattt tcaagagaag agaaaattct ttgcatctca tatgcccata
     1381 atgactttta accctggttt tttgcaagag tttttatcta agtgtgagct tcgctggacg
     1441 agatcggacc ttctgaacac ccggaacctg ccattcaccg ttgagaaccg cttgctttgg
     1501 ccaatagagg cgcatcatta aattgaactc tccttgcgga gcgggcagcc agttcgccag
     1561 ctttgctctg ccaggatttg tggcttgaat ataaatattc aaagagccat ccttcatcgc
     1621 tttcaactta ttcttgctgc ccaaagaata tcgatgcaat gggttttcca caaaaccctg
     1681 tcgctcattg tacaaagtca atgaccagaa agcgttgact ggaggccact gccctttggc
     1741 aaaatgcaaa atgtattttt gtgccccggt caatggtagg ccgtcagagt ccacccgtgc
     1801 cgtcggaaaa atagcatcct ccggaagatt tgcacccaat cccattcggg ccatcgccgc
     1861 gcgtgcatca tagttggttc cgtaattccc cacttccaga ccagaaaccc agccattcat
     1921 aacccgaccg gggatatatc gagccagctc ctccacccga atgtaaccct caagcaccgc
     1981 ttgatccaag gaactcttca cctctgaggg caatgatgca aagtccacgg tgtgcccagg
     2041 agtcacgccg attttctcta agctttccag catcacttga tcctccgacg atggagcatt
     2101 ctctttcatc aaataactca gtcgagttaa aaaggtctgt gcatccatga ccctcactat
     2161 ctccaccgga gtccccttga gatcgctctc agaccttaag tcagcaggaa tcggtatgtg
     2221 cgagaactga ctcagcggag tcaaacgaaa gcgattttga attgcatgca ccatgggata
     2281 atcttgaggg tctttgatta aagtatgacc caagatcaat gccatagacg tcggcgcctg
     2341 tatcctttga gcatttaccg gaatttcacc ctgccaattc ggactcacga gagcaaaatt
     2401 tcccgggcca ttgccggtgg ttcttgtccc aggcgaagcg aacacattcg tccaagcatc
     2461 gagaattgac atcatataat atcgctgtcc tgttgcggga acgcttaaca ccaccggctc
     2521 tttagaaagg tccacccaag cgacagaggc caaagtatcc acgctgggat ggatcgctcc
     2581 gctctccccc gcttcgggaa ttttgcggga atgacgaaat tcattcacga caatcccccc
     2641 agtcattact tgctgggaaa catccatcag cactaaaggg tatccataga ccacggcatt
     2701 ttgtacaaga aggcgaagtt cagaggactt caattgagtg aagagtttag cctcggcctc
     2761 tttcttcgct ttgattgcgg cctctcgggc cttttcttga agcacccgtt gctcttctat
     2821 tctttcttgc ctgagttgtt cttgtctttt tcgcgatgaa tgacatgcgt ttaaactgcc
     2881 caaccccatg cctatgagta aagatagaaa cgaaatgagg aataacttta aaagcataga
     2941 acccctccgc caatgccaca tctaatgtgg catcctctaa ctttgataca aagggatcgg
     3001 ggttcaactt gtataaaaac aaaaaaacag acttaagatt taatgtgaac taccactcca
     3061 actcaccaac ggcggctttc acggactgca gaatctcatt cttggtcaac atctctcgaa
     3121 tcactgctac gtcctttgca aaataacggt cttctttggc aaagggcact tgcttacgaa
     3181 tgtgatcata agcagcctgg acagcggctg tcggctttag gggagccagc aagtccagcg
     3241 cctgacaggc agaaagcagc tccatagaca cgacgttttc ggcattgcga agaatttttt
     3301 caaactttcg cgcggcaatc gttcccatcg aaacatgatc ttctttttcc gcggaagtgg
     3361 gaatggaatc cacgcttgcg gggtgagcca aaactttatt ctcgctgacc aaagaggccg
     3421 ccgccacttg cacgatcata tggccagaat tcaatccccc atttggagtc aggaacgctg
     3481 gaagctcact catttgcggc gaaatcattt tagaaattcg gcattcgctg atgctcgcct
     3541 gagaagaaat cgcgatcccc gcaaaatcca ttgcatgcgc caccggcata ccatgaaaat
     3601 ttccgcaaga tagaacttta ttatcgtcag caaaaaccaa aggattatcc gtgctcgaat
     3661 tggcttcagt ctccagaacc ttcacgatat agcgaagggc atctttcgcc gctccatgca
     3721 cggctggcat gcaacgcaga ctataagcat cttgcacgcg ctggtcatta gcaagatggc
     3781 tttcaccgat ctggcttgtg gcccccatca acttcaaaag attgcgcgcc gtttttgcct
     3841 caccgggatg agcccgcgta gcactgatca acggatcaaa aggtttgcga gagccacgca
     3901 ggccctctaa agacatcgcg ccggcaaggt ccgccatcca tagaagacga cgggcttccc
     3961 acgccgctag caaaccaatg gaagtcatca cctgacaacc attgatcatc gaaagacctt
     4021 ctttggcttt caactccaga ggctcaatat ttttttcagt caaaactttt ttaacagaaa
     4081 caatttcgcc cttttgccaa acttcgcctt caccaataac agccaaagcc aaatgagaca
     4141 gaggggccaa atcaccgctg gcccccacgg atccctgaca agggatcaca ggaataatat
     4201 cgttatttaa aaattctaga attttatcca ccaccacagg acggattcca ctatgtccgc
     4261 gggccaaagc attggcacgc aaaaccatca tcgcccgcga ttgaaccttg ctaaagggct
     4321 cgccgattcc catggaatga gaacgaatca agttgcgctg caattgctca atttctgaat
     4381 cagaaatccg cactgaagaa aaggccccga acccggtatt caccccatac atcacttcgc
     4441 cgtttttaat gcgaccttcg atatagtcac ggcttttttg catttccgcg cgcgccttcg
     4501 gcgccaactc agctttcatt tctggagtat gagcaatttc atgaagattt tcgagagtga
     4561 tattctcacc ggtgagtagg agggtttgca tagtcatcct ttacttttat taaaaccacc
     4621 aaagcgacgt tttttcccac cgccattacc gcttccacca ccggagcgtc tttttggtcc
     4681 ataaccggcc tgtctttttt caagggccgc tttggcttta ccaacgctgg tcaccgccgc
     4741 gttctggctc tccgtcgagt ggaaagggtg atcggtcatc acagtcacag tttggcgagt
     4801 ggttttttca atcgcaaata aatatgattt ttcttccgca gtacaaaacg caatggattt
     4861 tccctcagct cccgcgcggg ctgtccgacc aatacgatga acataactgt caggttgatg
     4921 aggcagctca aaattaatca cgtggctgat gccatcaata tccaaacctc gggccgcaat
     4981 atcagtggcg acgaggacgc ggatctttcc gctgcggaat tcttcgagag cccgctgacg
     5041 agcaccttgg gatttatccc cgtgaatccc cgccgcttga aagccttgct tcaaaagttt
     5101 ttcaacgatt cgattggctc catgcttcat ttgcataaag accaaaactt tatctaagct
     5161 gcggtccttc aagagatgaa tcaaaagatc gagctttttg cttttttcaa caaacatcac
     5221 cgtttgctcg attttctcag ccgttgtcgc ctgcggagtc acctccacct tacggggctc
     5281 atgcaggatg cggctggcca actgctgaac ttcacttggc atggtggccg agaaaaataa
     5341 attatgacgc ttttttgaaa gcaaaggaat aatgcgtttc acgtcttgaa caaagcccat
     5401 gtccaacatg cgatccgcct catccagaac aaagatttcc actttatcca gacgtaaatg
     5461 tttttgtcca aaaagatcca tcaagcgacc aggggtcgcc accagcacgt cgactcctgc
     5521 ggccagatca cgaacctgat tgccttggcc aacaccgccg aagatcaccg catgtttcat
     5581 tttcaagtgt ttactataaa tcaccaagct ttggtggatt tgaatcgcca actcgcgcgt
     5641 gggagttaaa atcagacaac gggggcattt cggctcgcga cgcacatggt ttttggctaa
     5701 aagttgcaaa atcggcaaac aaaaggccgc cgtttttcct gttccagttt gggccacgcc
     5761 caaaagatca tgaccttcca aaatcagagg aatagcctgc atttggatag gggttggggt
     5821 ttcgtaacca gcgtctttga gggacgcaag aagaggagct actaggggga gatcagtaaa
     5881 ttttgtcatt ggctctcttc tagcaatagc cagtcaggct ttcaaggaag aaccgtctgt
     5941 gctatgtttt acttagagga tttcagaaaa tcgatggctt ttttgtagtc attgcgaaac
     6001 acatagctgc cggcgaccaa gacatccgca tcttgacagt agctcagtgt tttctcgttg
     6061 acgccgccgt ccacttcaat caggtaagaa taatgatgct gctcgcgttt ttcccgcagg
     6121 atcttcattt tttctatctg atccatcata aaactttgtc cgccaaaccc tggctccaca
     6181 gtcatcacca gaaccaaatc cacttctgac aaaaaaggaa ggatatccct caaaggcgtc
     6241 cccggacgca gagtaattcc cgccttggcc cccaaagaac gaattttttt taaaacttga
     6301 tgaggatttt ctgttgcctc gacatgaata gttaaataat cgctgccggc tttaataaag
     6361 tcttcgacgt agcgttcggg cttttcaatc atcaaatgca catccagagg caaagggctg
     6421 acttttttaa gcgactccac cactggaatt ccgatggtca aattgggcac gaagtgaccg
     6481 tccatcacat cgacatgaac ccagtcggcg ccggccgtgg caatcgcttt gagttccctc
     6541 tcaagattgg caaaatccgc ggacagaata ctgggggcca ccagtttagt catagattta
     6601 ggattaaatt cagtttctac cttagattcc aaaatgatct cctgccttca gagggtggcc
     6661 cttaagaaag tcactcacct tcatgcgagt gcgggactca ggctgcactt cataaatatt
     6721 caaaatacca ctgtcacact gaaccgcaag aaattcttga tttacatcca tcaccattcc
     6781 cgtcttcgta ggggctccgg atttttcact gactttggtt ttgtgaattt ttattttttt
     6841 tccattcaac caagtataag taccaggtcc cataacaaga gctcgcacct tattgtgaat
     6901 ttccacagct ggcagcagcc aattgatttc accttccgat ttttcaatct tgtgagcata
     6961 ggtcacctgt gactcatttt gaggtgtcgg cactaaatta ccccgcatat aatccatcaa
     7021 ttccacagac aaaagatcgg cgccgagaac ggctagttta tcatgcaatt cctgcgcatt
     7081 gatttcatca tccaaggata aacgccgaat cccgataata tcaccagcat ctaatttttt
     7141 agcaactttt tgcaaagcta cgccagactc tttatctccg gcctccaagc tgcgctgaat
     7201 aggggcagcc ccccgccacc gcggcaaaat gctgccatga acattcaccg cgccaaaagg
     7261 aaacagatcg aggaattctt ggctgagaat ttgcccaaaa gccaccacca ccgcgacttc
     7321 cgccccccat ctttggattt cggctaaaat cagagggttt tttttcaaag actccggaga
     7381 gatcactcgc aatcctcgag cctgcgccag gcttttgaca ggactgggag tgagctgcat
     7441 cttgcgaccc gccggacgat ccggctgagt caccacaccc acgacttcaa agtgttcatc
     7501 atccatcagt gcttttaacg aggtcacggc aaagtctggt gttccgagaa aacagatgcg
     7561 taccttgctc acgccttaac actttctttt tttttggttt ttactgggac ggcttcgacc
     7621 tcagtttccg tctcttcttc tcccgcgtct tcgtcttttt tgacagggta gccgtatttc
     7681 ttaatttgat tcttgatttt atttcccttg ataaagctca agtgatcaat aaacaaagtt
     7741 ccttcaagat ggtccatctc atgttgcatg caaatggcca gcaggccatc agacttgact
     7801 acaatttctt ggcctttttc attgagcgcc ttgatctcca catattcaaa acgttccacc
     7861 gtctcgtagt accctggcag actcaaacac ccttcatcaa aagtcgtttt accttcgcct
     7921 ttaacaatga cgggattgat caataccaag ggttgaggaa ccgcctgctc caattccgtc
     7981 atctcttcgt attcataacg acgaccttct ttatcgcgtg gacgagtatc aatcaccagc
     8041 atgcgcacaa gttcgcccac ttgcggagcc gctaggccga tgccgcgagc actgtacatt
     8101 gtttcaatca tatcatgggc aagctgagtg tgtttcgcag tcactttatc gatctgttta
     8161 gaaacttcac gcaaacgagg atcagggaat tttaaaattt caaggatcat actacgacct
     8221 cccaaaaaaa agggtctaaa tagcggtaat acctgattat ctcttaagtc gcaacataaa
     8281 gaaataggcc actgttgcca tcaatagatt cggcgcccag gctgctaaaa taggatttaa
     8341 atgtccatga gtgcctaggg taatgaaaga gctaaagagt atccaataag caaataccag
     8401 cagaataaca attccgatat tcatcatcga acccccggag cgggccttgc ccacactgaa
     8461 gggaattccc agcaagcaca tcaccaagcc ggcgaaggca aagctaaatt tggaatgata
     8521 atccacctca tagcgaatcg tatccaggcc cgcctctttg ttacgctcaa taaaccttaa
     8581 aagttctgct tgattgagca tctccgaagt ttggccggtt gattgtaact ccttcgcgtc
     8641 ttcgcccatg acaattttct tggttttaaa ctggctggtt aatggaaaac tggaatttcc
     8701 cgtaaagaga gtcaccgaac cgtcaatcaa attccatgtc gagtccttca attccacctt
     8761 attggcagtg atcatctgca taagatccca ctctggactg aaaaaataca aagtcaaact
     8821 ctgggcagta tcccccttaa cactcaaagt tttgatgtta aaaatggcat ttttcgaacg
     8881 ataccaaatt ttatttgttt tcacgactga gaacagagaa ggcttttttt caatatcatt
     8941 ataaagaatg aaattctttt tccgcgccaa agccggcccc agccgatcca tcgtcacaaa
     9001 agctcctgcg gaaatcacgc tcacccacac caataaaggc aaagtgatgc ggaacaagct
     9061 cattccagaa gcaaaaaggg ccaccaattc attggcttta ttcatggagg aaatcgctaa
     9121 aatggtcccc aacaaacaag caatcggcag caatttatta aaaacctccg gcatatagta
     9181 aacataaaag ttcatcaaaa cagagagtgg aagttcctta tgactgacca tggtggacat
     9241 tgcatcgaca gccaaaaaaa tagtcacaaa aaccaggctg ccgccaataa aaaaacccca
     9301 aaataaacct gagacataac gatcgattcg attcataaaa ctattattgt tgactcaagg
     9361 ccaaattcct actttacttt gcttatggct ttacgcgtct tattagctga tgaaagttcc
     9421 accataaaac gagttatgca attggccctt caagattttg gagtggaggt aaaatccgtt
     9481 ccagtggggg tcgatgtttt gccgataagc aaaactttca aacctgatgt gatttttgcg
     9541 gatgtccttt tagccaaacg aaacggttat gaagtttgcg cagagctcaa agccgatcca
     9601 gagacaaaac atattcccgt cattttgatg tggagcagtt ttatggaagt ggatgagcct
     9661 aaaatctccg cagccaatcc cgacgcgcgc cttgaaaagc cctttgatgc tgaaactctt
     9721 cggcagatcg tccaaacctt ggcgccaaaa acgcaaagca atcctatcag cccttattta
     9781 caatttccga aaatgccgga gttcaatgaa acaccggttt caacccaagc ttcagcaaag
     9841 ccgtctctgc aaccgttaac ctcccccgag ggagcaatga aaaagccgat ggggaaaatt
     9901 ccagaagtgg ccccgattaa ttttgatcaa tttgatccca caacggcgga agaaaatatt
     9961 ttcagcatac ctgaagaaga cacttattcg ggaatttctc atttgagtca tgaaactccc
    10021 gaacaaaatc taagggccgt ggcaacagat gaagagtttg cgagcgttcc cttaaattct
    10081 tctcagcaaa aagacccact cgaagaagat ggatggcagc gtcaggattt gaaaaaattc
    10141 aaaatccagc cccccgttga ggccacgaga cccgctctcg atgagagggc cgacgaggag
    10201 gatttcgcta agaaatttgt catccctcag gaggcttcgg ataatgactc tcaccccgaa
    10261 gctgagggtg aattcgaaga gattcaattt ggcgaagatt taacctccac tcgtattggc
    10321 cctccaatgc agactaaggc ttctcctaaa gtttcgacca agtccatgct gcccccaggc
    10381 gtcaccaccg atgtggaaat gattgaacga gtcattcgcg atgaagctcg agaagtcatt
    10441 gaaagtatct gctggaaagt gataccagag atagctgaaa gaattgttcg cgaagaaatt
    10501 cacaaaatcc ttcgcgatgt cgaaaaatca aactagattt ttttaactaa aaactcaatc
    10561 ccttgaggtt gttatgactc tcttagaatt gattcgtgcc gcgatcaaag aagacatgcc
    10621 gtcaggcgat gtgaccaccg aagccttggc tttaaaacct catcacgggc gcgcacgctt
    10681 aaaagccaag gaagacatcg tgctctccgg cgccatggcc tttgaacaaa ccatgttgaa
    10741 tcttgaaccc aactgcaaag tgaaatggca tttcgaagaa ggcgatccca ttttaaaaaa
    10801 ccaaatcatc tgcaccattg aaggagatct ggtgcaggtt ctgaaagccg aacgtgtggc
    10861 tttaaacttt ttgggtcatc tttccggtat tgccaccttt acacgtcgtt ttgtgaaagc
    10921 ccttgaaggc accaaggccc gcgtcttgga cactcgcaag acccttcccg gtttccgtga
    10981 tcttgaaaag aaagccgtgg ttcatggcgg aggccttaat catcgcttaa atctttccac
    11041 cgccattttg atcaaagaca atcacatcgc cattatgaat ggccttgcgg ccgcggtcag
    11101 ccgcgtgcgc cagcacagcc aactgcccat cgaagtcgaa gcgggttctc ttgaagaagt
    11161 caaagtcgcg atcgaacaaa agccgcaccg tattctgctc gacaatatga ataatgagac
    11221 tttaaagcag gcccgcgcac tgattcccca ggaaattgaa actgaagcca gcggcaatat
    11281 gactttagaa cgcgttcgtt ccgtggccga aatagggatt gattttattt ctgtaggggc
    11341 tctgactcac tccgcgccct gcgctgacat cagtttggtt tttgactggg aggacacatg
    11401 caagactctc ccttaaacga tgtccgctta ggcaccatca ccaaaaaatg ggccgagagc
    11461 aaccgtctct ttgttcatta cgaagcctcc atgggcagca ccaatgatcg agccaaaggc
    11521 ctggcttttg aagagagact tctgcaagaa ggtctttgtc tttttttaac cgatgatcaa
    11581 acggcaggtc gtggccgcgg aaaaaatatt tggctcaatt ccaagcctgg aagtgccctt
    11641 ctgagttctt ggagttattt gttgaatatg aagccgcaac caaccacctc ttgcttagtg
    11701 ggactcgcgg tgtatcgagc cctctccacc acttggcctt ttctgaattg gaatatcaaa
    11761 gctccgaatg acatctacat cgacgataaa aaaatcgcgg gtattttact cgaatcggtg
    11821 atccagggcg atgagactcg actgatatta ggcctgggca tcaacgtatt ggcttcccca
    11881 gcagaagtgg gcaccgcaaa atccatactc gagtctttgc ctcctggcgt gcctctcctt
    11941 ggccaggatt ggaccgcttg cttggatcgc ttgatgtttg aaatgagtga agccatctcc
    12001 cactgtgaag aaccactgtc accttcagag caatactcct tattgatggc tttgaatgcc
    12061 catcctttgc tcaaagaaaa atacaaaggc cttgaaccca atggcacttt acttatggga
    12121 agcggcaaaa aaatcaattg gtgggagtta taaatggcgc tgacgatcac accttttcca
    12181 gaaattggct tggcctgtcc agacttcacc ttgcccgcag tggatggaaa aatctattct
    12241 ttaaaagatt tccaaaaagg gcatcctttg gtggtgatgt ttatttgcaa tcattgccct
    12301 tacgtgaaag ccattgaaga tcgcctcatt caactcggcc aggatcttaa tgctttgcat
    12361 gtgaatatcc tggccatttg ctcaaatgat gaagaaactt atcccgaaga cagctttgcc
    12421 aatcttaaaa aacgctggac agagaaatct tatccattcc cttaccttca tgacaaaacc
    12481 caggaagtcg ccaaggcgtt cggtgcactc tgcactcccg atttctttgt ttatgacaaa
    12541 gatctcaaac tggcctatcg cggacgcctg gataattctt ggcaagaggc cagcaaagta
    12601 acccaacgtg aactcttcga ggcggtgaaa ctgttagtgc atggacagcc agccccccaa
    12661 caacaaaccc cttcgatggg gtgctctatt aagtggttat aatatgaaat tattgagaag
    12721 ttttttaatt gcggttcttt tggtggtgat cagtatcggc gcttggttgt tttactattt
    12781 aggagccttt aaacccgtcg acattaggga agtgcaaaaa gaacccatga aaatgatcta
    12841 taaagaccac accggtcctt attataaaat cgtggccgtg attcaggaag tggaaacctg
    12901 ggcgaaggct cacaatgtcg actgctctga atctttcggc gaatatattg atgatgccaa
    12961 cgtagtggag gaggcccgcc tgcgcagtca tgggggctgt atcgtcaaag aatttccgcc
    13021 aaatcttcca gaaggaatta aaacccgtga agttccagct cgtaaatatg tcatggccat
    13081 cttcaatggc tcgcctggaa ttggtccgat gaaggtttac ccgaaagtag aaagttatat
    13141 gcacgagcac cacctaacca tggatggggc tatccttgaa atctatgtta tccattcaga
    13201 aaaggcgatg acgaccactt accttttccc tttagccgac tctctgccaa aataagaaac
    13261 taccacggat cattgccaaa aattaatttc acagacgtag ccactgagcc tgtgatcgga
    13321 gcactgaaaa ccgaaagtcc aggattcgcg gcaagggctg ccggatccac ttccaccgaa
    13381 aagaaaacat tgactgcctc agcaaccgtg caagtcgcca ttggaatcaa aaggggaata
    13441 taagccggat ctgaagaagc cggagccgtc gccgagagtg tcgctggtgg acattgggct
    13501 cgaaacgaag tgtggacaac aaataaacaa tcggggttcg tctgaacgca aggaacccca
    13561 aaggagtcaa aacgattggt ggcaaccacg ccaacagcag aggtgatttg caaaagacca
    13621 agaaggcttc ccgcagcatc catagccacc gttggtgaaa aaagcgcgag aggatattcc
    13681 tgattattta cgcatgcatt ggcctgggtt ccggcaacgc aattattaaa gtttacattg
    13741 aaaggggtca ctccatcgac ctgcgcggct ctcatggcat tacgaagcgc agcgggcatt
    13801 cccgcgatac ttcgcactcc tgaagaaatt ctgtcacgag tcgccatcgt tctgcccaac
    13861 tgtgtgctgt gattgagaga atcagaaaaa attaaactca tcactataaa aaccgagaga
    13921 agtccgatac aaataaggat ttcaagaata ctcatacctg aactatcccg taatttagaa
    13981 ttcatagaca gactccagga tccatatcca ccgtttgaat atatatcggg cctgaaacgg
    14041 gcttaagacg aacactttgg ccactgacgg cagcgggatt ttcatcgact gtatatacaa
    14101 tggaaacgaa ttcagcaggc gtgccattgc aagtaagcgg aggattttgg tttacagaag
    14161 caagatcggg tttacattga gcaaaaaaag aagtctttac acgcaccacg cattgcggac
    14221 tcgttggatc gcagggtgag cctgaagaat cataataaag ggggctttca atatttccat
    14281 ttgtaccata cagagtaaat gcaagataag gttgctgcac attcgcacag gtgccaacac
    14341 cacacacaca attataaaga gccacgtttt ccggtttttt tatagagagc aaaatagctt
    14401 ttttatttaa agcggtcatt tttaattgag tcacgatttg atcacgcgta attcgtaaat
    14461 tggtctgctg tgcacttttc agaaaggcca ttgccgcaat agcgaccgca gcggtgagca
    14521 atccaaacaa accagtggcg actaaaactt cgaccagcgt aaatcctgaa tttgaagaat
    14581 tatgaactga gctcattaaa aactgctcgg tgttgacgcg caagtcgtag tgggatcgca
    14641 tatgggactg agggcaacgg gaaccggagt actgccggga ctagccgtag gggttgtttg
    14701 ggttgtagtt gccgaggtag gctcaacata gttcgcctgc atctgggccg ctgtcgctat
    14761 gtcggtaacc ccttgggtga taatattaaa agcgacactt aaactcgtca cttccgcagc
    14821 cactataacg gaaagataag tttttaacgt cgcagaatct gtaattcctg cttgccaagc
    14881 gccagaaaca actcctcggg ttatgtcctg atttacagga taagcagcaa aagaagcagc
    14941 gagggcttgg ctcttggctg gatctgtaac atggtattgt gcgagggcgg ccaagagaat
    15001 tggattagtg attccagcca gggaatttac gagagcctga gccagagtga ggtcgttaat
    15061 ccccgccccc cccagtgccg tagccaataa gggatccgta atccaggagg ttgccgcaga
    15121 tatactgttc acctcggctg cggaaatagt gtacccaacc tggtgtgcca tcatatagga
    15181 aactgcacct agcgatgaat aggcggcctg cggattcagc aagagaaagt cactataggc
    15241 agcaaccaga gaggggtcag taattttaaa attattaaaa aggttgtccg tgaagttatc
    15301 tatattcgag acttgagacg taccaatgaa cttactttgg acgatgtctc gtacctgtat
    15361 gtaggtaggg tttgtggtgg tgacacctgg ctgtactgaa actaagctcg ccaaggtgaa
    15421 tttattagga acataaccat aagctggcgg aagaatatct tttgtctgaa tattaggagc
    15481 aaatttatca atggtcgcca aaatagttct ttctttgact cctgaaaaaa cattattagg
    15541 aattcgaatc acgtaatgaa tgctgataga ttccgccgca ggacaaggag agccgacggc
    15601 tgccggacat tttgcggtga aggtcgtaaa cacttcgaaa gggcaagcca ggcttgcgct
    15661 ggttgcaccg gtttggcaaa gattgcctct cgtgtcatag agtgcaggtt gataagcatt
    15721 atcgctgact ggccccgccg tcgtcagtcg ctgagctcca gtgtttgaaa gaaaagccgc
    15781 gtagaggctc acgggatatt cggtgccatc accataacaa gggaggctac cgttgcccaa
    15841 aacacagttc tgaagcgctg aattatcagt ggtcgcaaga ctattacgaa atatagccgg
    15901 aaggctggaa tatctttcga tctgatagac aatagcatct cggctttctt taatgcgcgc
    15961 ccccgaaata aaagcattcc cttgtgccat ttttccaaat ccagtggttt gtaaaaaaac
    16021 gccaatgaca atcaccccag ataaaatacc gagaacaata tttccttcgc gattatgcaa
    16081 tggatttgga aatcctaacg aagacttcaa tttatattta ttttgcatat tggaattaat
    16141 atacataaat gatgcccttg ccttgaattt tgaccttttc ttgtgaaagt atccttcctt
    16201 cttgtcggca aattatttat tttctagacc taaattttta aatatttaat tatttagcta
    16261 ttttggctca atttgggatt ctctcatctt gaatatctaa atctgagtct atcaggcttt
    16321 atttataaat cggttgcaat taaaaaatgc ctattctaaa aatgagatag tttcatggag
//
LOCUS       DATBTE010000296         4545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2020312, whole genome shotgun sequence.
ACCESSION   DATBTE010000296 DATBTE010000000
VERSION     DATBTE010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2020312"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VIG33_11025"
     CDS             <1..398
                     /locus_tag="VIG33_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEY8271410.1"
                     /translation="TYELSYSSVGFIEACVQTYGQETIQNLEPCGPAFSKDFNTQPQS
                     LKMQLNPRDRLVIATEGLILSQDSKGQNWGGENLRQAIRTAPRKGVHELRNEILLQNE
                     NFTGRLTPLRDQTVIAMEVKDRVIKLAKN"
     gene            470..1609
                     /locus_tag="VIG33_11030"
     CDS             470..1609
                     /locus_tag="VIG33_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEY8271411.1"
                     /translation="MADVTIYEGALDKGLEGVVACTTKVSFIVGDALNFRGYVIDDLA
                     AHSTFEEVTYLLWNDTLPNAQELSAFSAELHKEMALSAEFIKVLKTIPTNVHPMGWLR
                     TAVSLLAHWDADANDNSLEANHRKSIRLTAKMGPLLCAFDAIRKGKEPVSPKMEKSIA
                     WNMMYMLGGGKEPQAEHVKVMDTCLILHADHELNCSAFATRVTASSLSDLHSAIVSAI
                     GALKGPLHGGANEQVILMLQKIGTMEKAQQFVKDALSAKEKVMGIGHRVYKNGDPRAR
                     ILRGMSDKLTQDAGIHQMYEMSTLIDDTMYKEKGLMPNVDFYSATVYFAMGITTDLFT
                     PIFAASRISGWCAHAFEQYANNRIYRPRGKWAGKEGLTWKPISQR"
     gene            1693..3228
                     /locus_tag="VIG33_11035"
     CDS             1693..3228
                     /locus_tag="VIG33_11035"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEY8271412.1"
                     /translation="MESFCKLTFTLVLFIFFLILPPVFAQESLGLSYNTVIDALSLDE
                     MRAAVFRIQEHGLNPRNYWSEGMDGQYQKGEYEQLKPLANQTFLRLLADISLGTIDPE
                     KLSPDIKLTRKKFLTPDRLRALIVSTGQKPELLIAALAPQNTLYEALKHSLAKVYPAC
                     QNGKWVKLTPSKKILKLGVKSPAVIPLKKYFTFLGYQISNLDDVVDQETVRAINDIEW
                     TLHLKPDGKLRSSGKVWKFLNTPCMDRVHQLQADMEKIRWFPRQFEDRYIFINLAMTY
                     LVMVDKSQNPPYIMSFRTINGRVERKSPTMKDRVVRVILNPFWVVPPTIFVEDKVKEI
                     RHLSRRQVSEYFNSHNYEVWNKSFTKRLNPNSINWWALNTSLDSEIYIRQKPHYMNAL
                     GVIKFELTNSFSVYLHDTNQRELFLDPQRLVSSGCIRVEKPLDLAEYLLKNSGWDRTK
                     IESVIAKPGQVMKTETPVIVQNPIAVYTMFLTSFLSSDGVLRFAEDAYEQNKTILKTL
                     QRL"
     gene            3298..3696
                     /locus_tag="VIG33_11040"
     CDS             3298..3696
                     /locus_tag="VIG33_11040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEY8271413.1"
                     /translation="MASVFIKIICGEVPCYKIYEDEKIFSFLALDQVNLGHTLVISKE
                     EINHWTEVPPEIYAHLHKVSQKIGKAILKASGSPRVGQIVAGFEVPHYHLHLIPAWSI
                     PDLDFKRAQRRSDTEMKQVQAAIVKYLENI"
     gene            3893..>4545
                     /locus_tag="VIG33_11045"
     CDS             3893..>4545
                     /locus_tag="VIG33_11045"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY8271414.1"
                     /translation="MSESDNKESQIIELDFDFNFNEAQAISEVFFKESEEILSDLDEH
                     ILRLEAHPHDEVLIQGLFRRVHTLKGSVGAVPGGQLLGSLAHEFETLLDRLRKQKIQP
                     TKECIEIFLHSSRLLKILAGNLRTHHEIYPEELSEVIELIACYGSFQLNVSPESTLVS
                     EKVFSSEFQETEGVRNKDGVWLSKEQMSSLLQLSSDFILLKNSFQPLLEKDQEKQGSV
                     "
BASE COUNT         1322 a          919 c         1001 g         1303 t
ORIGIN      
        1 gaacttatga actgagctat tccagcgtag gcttcattga agcctgcgtc caaacatacg
       61 gccaagagac catccagaat cttgagccct gtgggccggc tttttcgaag gactttaaca
      121 cgcagccaca gagtttaaag atgcaattaa accctcggga tcgccttgtt attgcaacag
      181 aggggttgat cttatcccaa gattctaaag gacagaactg gggaggggag aacctgcgac
      241 aagccattcg cacggcgccg cgaaagggcg ttcatgagct gcgaaatgaa attcttctac
      301 agaatgaaaa tttcaccgga agattaaccc ccctcaggga tcaaacggtg attgcaatgg
      361 aagtcaaaga tcgagttatt aagctcgcaa aaaattaagt atgtgacttg tactgtgatt
      421 ttttgataag acgtgactgt ttaattacat ggttgaaagg atactcaaga tggcagatgt
      481 gactatttat gaaggagctt tggataaagg tttagagggc gtagtagcat gtacaactaa
      541 agtttcattt attgtcggtg atgctttaaa ttttcgcgga tatgtcattg atgacttggc
      601 agcccattca actttcgaag aagtgaccta tttactttgg aacgacacat taccaaatgc
      661 ccaggagctt tcagcatttt ccgcggaact tcataaagaa atggctttga gtgctgagtt
      721 tatcaaagtt ctaaaaacga ttcccaccaa tgttcatcct atgggctggt tgcgtactgc
      781 agtttctttg ttggcccatt gggacgccga tgccaatgat aattctctgg aagcgaacca
      841 tcgtaaatcg attcgcttga cggcaaaaat gggacctctt ttgtgtgcct ttgatgctat
      901 ccgcaagggc aaagagcccg tatccccaaa aatggaaaag tcgatcgctt ggaatatgat
      961 gtacatgcta ggtggaggca aagaacctca ggcagagcat gtgaaagtga tggatacctg
     1021 tttaattctt catgcggatc atgagctcaa ctgctcagct tttgcgactc gcgtaaccgc
     1081 atcttcattg tctgatcttc actccgcaat tgtttccgcc attggggctt taaaaggccc
     1141 tttgcatggt ggtgcgaatg aacaagtgat cttaatgttg caaaaaatcg gcaccatgga
     1201 gaaggctcag caatttgtaa aagatgcctt aagcgcaaaa gaaaaagtca tggggattgg
     1261 tcaccgagtg tataaaaacg gagacccacg ggctcgtatt cttcgaggta tgtctgataa
     1321 attaactcag gatgcgggca ttcatcaaat gtacgaaatg tcgactttga ttgacgatac
     1381 tatgtacaaa gaaaagggtt taatgccgaa tgtggatttc tattctgcaa ccgtttactt
     1441 tgcgatgggc attactacag atttgtttac ccctatcttt gcagcctctc gtatttctgg
     1501 ttggtgtgct catgcttttg aacagtacgc caataacaga atttatcgtc cccgtggaaa
     1561 gtgggccggc aaagaaggcc tcacttggaa gccgatttct caaagataaa tagtcatatc
     1621 taggaaattt caagctcctt tccttgggtg ttccagtgga tgtttaaaaa tggtggtgtc
     1681 ctaggagaca ccatggaatc tttctgcaag ctcactttca cattagttct gtttatcttc
     1741 tttctgattc tccctcccgt ctttgcacaa gagagcttag gcctaagtta taacacagtc
     1801 attgatgcgc tttctttaga tgaaatgcgc gcggcggttt ttcgcatcca agagcacggt
     1861 ttaaatccac gcaactattg gagcgagggt atggacgggc aatatcagaa aggcgaatat
     1921 gagcaactga agcctttggc aaatcagact ttccttcgcc tccttgcgga catttcctta
     1981 gggaccattg atcctgaaaa gctttcacct gatatcaagc tcacgcggaa aaaattttta
     2041 actcctgacc gattgcgcgc tttgatcgtg tccacgggac agaaaccgga gttattaata
     2101 gctgcattgg ctccgcaaaa caccctctac gaggctttga aacacagtct tgccaaagtc
     2161 taccccgctt gtcaaaacgg gaaatgggtg aaattaaccc cttcaaagaa gattttaaag
     2221 ttaggagtta aaagcccagc ggtgattccg ttgaaaaaat attttacctt tttaggctat
     2281 cagatttcca atcttgatga tgttgtggat caagaaactg tgagggctat caatgatatt
     2341 gaatggactc tgcatttgaa acctgatggc aaactgcgtt cttctgggaa agtgtggaaa
     2401 ttcctaaata caccctgtat ggacagagtt catcaactgc aagccgatat ggagaaaatc
     2461 cgctggttcc caagacagtt tgaagaccgc tatatcttta taaatctggc gatgacctat
     2521 ttagtgatgg tggataaatc tcaaaatcct ccttatatca tgagttttcg caccattaat
     2581 ggacgagttg aaagaaaatc ccctaccatg aaggatagag tcgtacgggt catcttgaat
     2641 cctttttggg tggtcccgcc gacgattttt gtggaggata aagtaaaaga aatccgtcat
     2701 ctttcacgca ggcaagtcag cgagtatttc aattctcaca actatgaggt ttggaacaag
     2761 agctttacca aaaggctcaa tcctaacagc attaattggt gggccctgaa tacatccttg
     2821 gactcagaaa tctatatccg tcaaaaacct cattatatga atgctctggg agtgattaag
     2881 tttgaactta caaattcgtt ctcggtctat ctgcacgata ccaatcaacg agagctgttt
     2941 ttggacccgc aaagactggt gagttcaggt tgcattcgcg tggaaaagcc cttggatctg
     3001 gcggaatatc tgcttaaaaa cagtggatgg gatcgaacaa aaattgaaag tgtcatcgca
     3061 aagcctgggc aagtcatgaa aacagagact ccagtgattg tgcaaaatcc gattgccgtc
     3121 tacacgatgt tcttaacttc tttcttaagt agtgatggtg ttctgcgttt tgctgaggac
     3181 gcttatgaac aaaataagac tattctaaaa acgctgcaaa ggctgtagct tttcataaaa
     3241 atccgtgaat ggaaaagaaa aatgtaatag actccttatc atttagagga gaaattcatg
     3301 gcttcagttt ttataaaaat tatttgtgga gaagttccct gttacaaaat atatgaagac
     3361 gaaaagattt tttctttttt ggctttagat caggtcaatc tgggtcatac tttggtcatc
     3421 agtaaagaag aaatcaacca ttggaccgaa gtacctcctg agatttatgc tcacttgcat
     3481 aaagtttctc aaaaaatcgg taaagccatc cttaaagctt caggcagtcc acgagtagga
     3541 cagatcgtgg ctggatttga agtcccccat tatcatcttc atctgattcc cgcctggtct
     3601 attcctgatc ttgatttcaa aagagctcag cgtcgttcgg atactgagat gaaacaagtc
     3661 caggcagcta ttgttaagta tttagaaaac atttaaatat ttattttagg tgaaattaaa
     3721 ctgactggcc tggtcgaaag gccctaagcc gttctgccac tcagcactcg cctatgttaa
     3781 atttgactaa attccgcata aatttaattc tattgtgtta agccaactct ctgagattcc
     3841 gatagcttat ccagaaaact aaaatggatc gcaaggcagt tgggtaaatt tgatgtcgga
     3901 atcagacaat aaagaatcac aaatcattga gctcgacttt gacttcaatt ttaatgaagc
     3961 gcaagccatc tcagaagtct tttttaaaga gtctgaagag atcctctcag atcttgacga
     4021 gcacattctg cgtttagaag ctcatccgca tgatgaggtc ttgattcagg gcctttttag
     4081 aagagtccat acgcttaaag gcagcgtagg cgccgttcct gggggacagc ttttgggatc
     4141 cttagctcat gagtttgaaa ctctcctgga tcgtcttcgt aagcaaaaaa ttcaaccaac
     4201 caaagaatgc attgaaatat ttttgcacag ttcaaggctt cttaaaattt tagctggcaa
     4261 ccttcgcaca catcatgaga tttatcctga agaactcagt gaagttattg aacttatcgc
     4321 atgttatgga agtttccagt tgaatgtttc ccccgagtca actcttgtgt cagaaaaggt
     4381 ttttagcagt gagtttcaag aaacggaagg ggtgcgcaat aaagatggtg tttggctttc
     4441 gaaagagcaa atgtcttctc ttttgcaact gtccagtgat tttattctgt taaaaaactc
     4501 ctttcaacct ttgctggaaa aagatcaaga aaagcaaggc tccgt
//
LOCUS       DATBTE010000297         2676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2294051, whole genome shotgun sequence.
ACCESSION   DATBTE010000297 DATBTE010000000
VERSION     DATBTE010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2294051"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1864)
                     /locus_tag="VIG33_11050"
     CDS             complement(<1..1864)
                     /locus_tag="VIG33_11050"
                     /inference="COORDINATES: protein motif:HMM:NF005829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEY8271415.1"
                     /translation="MRNTVIIAEKPSVARDLARAVGAAKQGEGSFYGNGYTVTWAIGH
                     LVTLPEPHQINAAWKAWNFALLPMLPAKWPLVAVEKTQSQFEIVKKILLDCDEVVCAT
                     DAGREGELIFRYIYELAKCKKPVKRLWISSLTPEAIQAGLRSLKPASAYDSLADAARA
                     RSRADWLIGMNLSRAYALTTNEQLFVGRVQTPTLAMVVERDLKIQNFKPEDYIVIEAQ
                     FQVAEGAYRGFYIGEKEEFSGPSSNPNTAKEKRLPVDPKKVELLLSRILQNEALVKSV
                     DGKTVKLPPPQLYDLTELQRHCNRLFGFSANQTLSVAQSLYERHKLISYPRTGSRHLS
                     QTVAGTLPEIVEMIRSPYESLLVESTGRAPLSKRFVDDAKVTDHHAIIPIASPGSRAS
                     SSLSSDEQKVFDLICRRLLMAWQRDYVTAVTTVITEVGRLDLFKTQGTVVVDLGWKAL
                     DIKGKASSSSSQEPEVPVGLAPGVAVKVLKVESRQKRTEPPPHLTEATLLTGMETAGR
                     NLEDRELAEFMQDSGLGTPATRAGIIEALLARNYLMRREKSLIATPLGHKLIEVVHPS
                     VKSPELTARWEQMLAEIQSKKSTLTDFMVKLEHEVSARITEIAESPRPQRVSSPD"
BASE COUNT          713 a          679 c          593 g          691 t
ORIGIN      
        1 cgtcgggtga ggaaaccctt tgcggtcgcg gtgactctgc gatttccgta atacgtgctg
       61 aaacttcatg ctcaagcttg accatgaagt ctgtaagagt gcttttttta ctttgaattt
      121 cagcgagcat ttgctcccat cgggcagtca actcaggact cttaacgcta ggatgaacaa
      181 cctcaatcag tttgtgaccg aggggtgtcg cgatcaaaga cttttcgcgt ctcataagat
      241 aattgcgcgc caagagcgcc tcgataattc cagcacgagt cgccggagtt cccaatcctg
      301 aatcctgcat gaactcggcc aattcgcggt cttcaaggtt gcggcccgcg gtttccatac
      361 ccgtcagaag cgtcgcctca gtaagatgag gaggaggctc cgtgcgcttt tgccgagatt
      421 ccaccttaag gactttcaca gctactcctg gcgccaaccc gaccggaact tcgggttcct
      481 gcgaggaaga gctgctcgct ttccctttga tgtccaaggc cttccaacct aaatccacca
      541 ccacggtgcc ttgagtttta aataaatcca atctgcccac ttcagtaata accgtcgtca
      601 ctgcggtcac gtagtcccgc tgccaagcca tcagcagccg acgacaaatg agatcaaaaa
      661 ctttttgttc atcactactt aagcttgaac tcgctcttga gcccggagaa gcaattggaa
      721 taatcgcatg gtgatccgtt actttggcgt catccacgaa tcgcttgctc aaaggcgcac
      781 gaccggtgga ttccacaagc agagactcat aaggggaacg aatcatttcc acaatttccg
      841 gcaaagtccc tgcgaccgtt tgagaaagat gacggctgcc ggttctagga taactgatca
      901 acttgtgccg ctcataaaga ctttgagcca cgctcaatgt ttggttggca gaaaaaccaa
      961 aaagacgatt gcaatggcgc tgcagttccg tcagatcata aagttgcggc ggcggcagct
     1021 tcaccgtttt cccatcaacg gattttacca aagcctcatt ttggagaatc cgacttaaaa
     1081 gcaattccac ttttttaggg tccacaggca atcgtttttc ttttgccgtg tttggattgg
     1141 aactgggacc tgagaactcc tctttttcgc ctatatagaa cccgcgatag gcgccttcgg
     1201 caacctgaaa ctgggcttca atgacaatat aatcttccgg cttaaaattt tgaattttta
     1261 gatctcgctc caccaccatc gccagcgtcg gagtttgcac tcgacccaca aaaagctgtt
     1321 cattggtggt gagggcatag gcacgagata gattcatacc gatgagccag tccgcccggc
     1381 ttcgagcccg tgcggcatcg gccagggaat cataggcgga agcgggtttc agagagcgta
     1441 aaccggcctg aatcgcttct ggagtgagag atgaaatcca taaccgctta accggctttt
     1501 tgcattttgc gagttcatag atataacgaa aaattaattc gccctcgcgc cccgcatccg
     1561 tcgcgcaaac cacttcatcg caatcgagaa gaattttttt gacgatttca aactgagact
     1621 gagttttttc caccgccact aacggccact ttgcgggaag catcggcaac agagcaaagt
     1681 tccaagcctt ccaagcagca ttgatttgat gaggttcagg caaagtgacg aggtgaccaa
     1741 tcgcccaagt tactgtgtaa ccatttccgt aaaacgagcc ttcgccttgc tttgcagcac
     1801 ctacagcccg cgctagatca cgggcaaccg atggtttctc agcaataatg acggtgtttc
     1861 tcatacttgc tagaaaacca tgacctaacc aagagttcaa atggaagcaa tgatttcaag
     1921 taaaaatcct ttcctttaaa gggaaaaacc gaatctcgga aatattattg aggaccacgc
     1981 ctcattttag actgcatgcg gtctaaacgt atattccgca ataatatcat aaatctatgg
     2041 tgcttcatcg cctgctataa caaagctggc taggctcaat aaaccaggac tttgcgacta
     2101 atgaccgcat tacttctcta agttctaaca cgaaaacgag ctaatggaag ctctcaactc
     2161 gttgtacatt tacattgaaa aagtgcgttg ctacccttga tctaataagg aggcgccata
     2221 tcgatcttaa agaattactg gcaaaagctg aaatgactgc aaatacatat cgcgaggaaa
     2281 agttagatgc tgatagctct ctacaaatgc ttgcgagcgc ctacgctaca ttatttttga
     2341 aaacccaagg tggaatgtct actcgagaag tcgcatttgc acataagtta atagcggatt
     2401 gtctggatac ccttgatcaa ccacatttgg caggccaaat gcacacatga gcccttgctt
     2461 tgcagcccct acggcccgcg cgagatcacg ggcaaccgat ggtttctcag aaacaatgac
     2521 ggtgttctca tttatcccag ggaaaaccgc cggatctcgg aaatacggct ccttgaatct
     2581 agctttttaa caaaaattct cgactgggaa ccatctggat tatcatctgt tttatattta
     2641 gggtttcttt tctatttttt gttacaatag tacctg
//
LOCUS       DATBTE010000298         1927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1610353, whole genome shotgun sequence.
ACCESSION   DATBTE010000298 DATBTE010000000
VERSION     DATBTE010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610353"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..1550)
                     /locus_tag="VIG33_11055"
     CDS             complement(207..1550)
                     /locus_tag="VIG33_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEY8271416.1"
                     /translation="MDALKETFNKIVEEARQDGAKVELLISGGENLSLEYSQRKLEKF
                     ESTQSQMAGLRVALGASQGYAYTENLSKEALLRTYKEALSNAKTLKSMETQEIPFAKP
                     QPIINMTELFNPEEISMDKKMEAAKLLEEVSLKQDPRIQSVPYSGYNEGLSFRRVLNS
                     EGLDQEFKQNYYSGYSYSLAKEGDSSKMGGESFFARSFKDIKVEEVAKEGVRKALSLL
                     GATKLKTGNYAVVIDREQFRTVMMMFAGYLSAKDVHEEKSLFKGRLNQKIANEKFQLI
                     DDPFETRGTSVRPFDSEGAASQKTVLFEKGVLKNFLTNLEYAKKLNLPHTAHASRTPS
                     SAMDIAPTNLVLAKGTKTLQELLSKHDRVVYLTEFTGSLHSGFKESTGDFSMPAEGFL
                     YNHGKLVGPIDQFVMSGNILDLLLDIEDLGNEYAKPGSSLICPDVLIKSLSFAGA"
     gene            complement(1550..>1927)
                     /locus_tag="VIG33_11060"
     CDS             complement(1550..>1927)
                     /locus_tag="VIG33_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY8271417.1"
                     /translation="YKYAPASRMRNTFIDAGKDSFEEMIRDVDYGLYAKNMGGGSVNP
                     GTGDYNFQVREAYIIRNGRVEEAVKGACLIGRGIDTLGKITKVSKDLKLATGMCGSVS
                     GTIPAAVGQPQILVSSLMVGGRA"
BASE COUNT          517 a          476 c          366 g          568 t
ORIGIN      
        1 cacgtgatat ctcttcggct atctggaaaa aatctgcagg tcttgctctt cattttattg
       61 ttgctgttga gctgctgcag ttagattttt ttgataggtt ctgttaaggg cttggtacta
      121 ccaaagtcct ggtaagtggg caaccaggca taaacttgga gttgcgagca aggacgaagc
      181 tttcagggcc accttttcat aaaaccctat gcccccgcaa agctcaggga tttaatcaag
      241 acatcagggc aaatcagcga actccccggt ttcgcatact cattgcctag atcttcgata
      301 tccaaaagca gatccaagat attccccgac atgacaaact gatcaatggg ccccaccagt
      361 ttcccatggt tatagagaaa gccttccgct ggcatagaga agtcccctgt ggactcttta
      421 aagcctgaat gcagtgagcc tgtaaactcg gttaagtata ccaccctatc atgtttggat
      481 aacaactctt gcaaagtctt cgtgcctttt gccagcacta agtttgtcgg agcaatatcc
      541 attgccgagg aaggagttct agaggcatga gccgtatgcg gaagatttag tttctttgca
      601 tactccaaat tagtcaggaa gttttttaaa actccttttt caaagaggac ggtcttttga
      661 gaagcagccc cttcggaatc aaaaggcctc actgaagtcc ctcgagtttc aaaaggatca
      721 tcaatcaatt gaaatttttc attggcaatt ttctgattga gccgcccttt aaataaagac
      781 ttttcctcat gaacatcctt tgccgataag taacctgcaa acatcatcat caccgtgcgg
      841 aactgctcac gatcaataac aaccgcatag ttcccggtct taagttttgt ggctcctaaa
      901 agggagagcg cctttcgcac gccttctttg gcaacctctt cgactttgat gtctttaaaa
      961 gagcgcgcaa aaaaagattc tccacccatt tttgaactat cgccctcttt agctaaagag
     1021 tatgaatagc cggaataata gttctgctta aattcttgat ccaaaccttc cgagttcaga
     1081 actcgcctaa aacttaatcc ctcattatat ccagagtaag gaactgactg aatgcgcgga
     1141 tcttgcttga ggctcacttc ctcaagcaac ttcgcggctt ccattttttt gtccatggaa
     1201 atttcttcgg gattaaaaag ctcagtcata tttattatcg gctgaggttt tgcaaaaggt
     1261 atttcttgag tctccataga ctttaatgtc tttgcattac tcaaagcttc tttataagtg
     1321 cgaagtaagg cctctttcga caaattctct gtgtaagcat agccttgact tgcccctaag
     1381 gccacacgca agccagccat ctgtgactgc gttgattcaa atttttccaa ttttctttgg
     1441 ctgtattcca aggaaagatt ctcaccacca gaaattaaaa gttcaacttt tgcgccgtcc
     1501 tgacgggcct cttcaacaat tttattaaaa gtttctttta aagcatccat tatgctctac
     1561 cccctaccat gagacttgaa accaagatct gtggttgccc aaccgccgca ggaattgtac
     1621 cactcactga accgcacatt cccgttgcca atttcaagtc ttttgaaacc ttggtgattt
     1681 tcccaagtgt atcaatgcct cgaccgatca agcaggctcc tttaaccgcc tcttcgaccc
     1741 gcccgttacg aatgatgtaa gcttctcgta cttggaaatt gtaatcaccc gttccaggat
     1801 ttacggaacc tccacccata tttttagcat aaagcccgta gtccacatct cgaatcatct
     1861 cctcaaagga gtccttaccc gcatcaataa aagtgtttct catacgggag gctggagcat
     1921 atttata
//
LOCUS       DATBTE010000299         4912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_39327, whole genome shotgun sequence.
ACCESSION   DATBTE010000299 DATBTE010000000
VERSION     DATBTE010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39327"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            761..1282
                     /locus_tag="VIG33_11065"
     CDS             761..1282
                     /locus_tag="VIG33_11065"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEY8271418.1"
                     /translation="MNKVKMSVLILTMVSAGLLAKAQTLTDGQIVQIMKTANDEEADL
                     GKMAKSRADNKYVKEFAKTMVDSHKQSEKDVKDVASKAKIKAVETDASKNLERDIKAK
                     EKDLKKLKGAEFDKAYIDQQITLHQQLLDDLNSKFIPAAQSPELKVYLETTKAHVQEH
                     LSKAREIHTSLTK"
     gene            1368..1811
                     /locus_tag="VIG33_11070"
     CDS             1368..1811
                     /locus_tag="VIG33_11070"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY8271419.1"
                     /translation="MNFLKKLIRTEDDLVFFILRFVLGVVFFPHGAQKVLGWFGGGGF
                     TGTMGFFTSQGIPAFFALMAILAEFAGSLGLILGLLTRVAAFGITCVMMVAIFTVHLP
                     NGFFMNWTGKQAGEGFEYHLLVLAISSVLMWKGAGAYSLDRKLSS"
     gene            complement(1821..2996)
                     /locus_tag="VIG33_11075"
     CDS             complement(1821..2996)
                     /locus_tag="VIG33_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271420.1"
                     /translation="MESFFGNLHPALAQTWQKLGSIVTIKGPRLTPRGTLRKLPTIAC
                     GDNYHIATLRSDGLVFSSGTNTKGQLGDNTTVAKSTFVQATGISNAVALACGLDFTIA
                     LRADGLVFGCGYNSSSGPIGDNTTLDRSTFVQGIGVSRVVAISCGSHHTAALRSDGLV
                     FTSGSGGTGQLANNTIVDQHTFVQAIGVSNAAAIACGRNTTAAIRSDGLVFACGDNAG
                     GQLGVNSTASKLTFTQAVGISNAVTIGISVGNFTVALRSDGLVFACGVNSKGQLGDNS
                     ASTASKSTFVQALGISNAVAIACGYDHAAALRADGLVFTCGYNNKGQLGINSTVNKSS
                     YVQASGISNAVAIACGKDYTIALRADGLIFGCGNNANGQLSINSTTDKSTFVQATGI"
     gene            3263..4723
                     /locus_tag="VIG33_11080"
     CDS             3263..4723
                     /locus_tag="VIG33_11080"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY8271421.1"
                     /translation="MRFIILLIIFMSSACATKIRQPNAVDAPANSLELEQALLQGAAP
                     TLAFLKTQTSLKSFEGLNSRDPAIYIPKTLKAEKSFPISKEKNLQQHFLRRFRGWNLK
                     QKIKREHELLADFNCGKAVETQALGFSFELDFPEAEASAGSRALHEQVLSCATFPRQE
                     SLFRLAVFSIHGGECPQAMKYLDMFPATPERGVSDRISYLRSLCSTFPIAKNGNPWGG
                     YGSLLGDIKGGEEPSSPKPKWYLSTSSGSEEWDRLLVSFIELSEKNEGATVQYISSKL
                     NFEKFRSLPLAFQTSMLVMMSFNGADLSVFQTLHRYLSDHPETISPAVAGLLFPIRYW
                     KEIIANSKSADPILVKALIRQESAFNPTARSRAKAAGLMQLIYPTAKFFGITQPKQLL
                     APEANIHAGSEFLSQLINQFGSVELALAAYNAGPATVRDWQRRYPTNNIDLFVEMIPY
                     SETREYVRLVRRNYKVYQSILMKTQYIGDLSSVGTE"
BASE COUNT         1371 a         1011 c         1066 g         1464 t
ORIGIN      
        1 ttttttcgca aaaactgagt ctcaaatgaa ccgtcaggtg gctaccttca actcagcttg
       61 ttattgcctg agttgcactg gagaaaattt cgggggaact tagtatctat gtttatttct
      121 atttttcttt tttgctttgg aagccctggc cttcagtttt ttcccctggc ttcttgagct
      181 cgcgacgttt ctttttttca tatactttga tgcgttcgcg tagccacctg atttttttct
      241 ttttttagag acatgagatt tagaaacatg gggctttttt ttctttttta ggtgcatact
      301 ggcgtttgat ctggaatgct tttttgtttt ggcaaaagcg acttctccag aaaacatgag
      361 tgtcattgct aagaaaaata cggtcattat ttttaaaatt aaattcatga atcaccttcc
      421 cctagggtct agttgttttt tcggcaggcc taaggtcaaa cttgagtaga attagagatg
      481 ccaaatattt aggcgctcac gggaaaacgg cgattcaaat tgagaatctg tgaagcaagg
      541 attcacgacc ctttgcaaag tgtttataca ctttaataca tgtgttgttc tgggagtctt
      601 ctgattcctt gcaggaagct gtactgtcta atcacaaaat gcgttattgg gagtttaggg
      661 tattgacgat ttattcttga gaccttcgtt ctgaatgtaa tgtttttcag cgatctttcc
      721 agtgctttaa atacattatt tttttaaagg agaacgaaaa atgaataaag tgaaaatgag
      781 cgtcctaatt ttaacaatgg tctctgctgg tctcttggcc aaagcgcaaa ctctgacaga
      841 tggccaaatt gttcaaatca tgaaaacggc gaatgacgag gaagctgact tgggcaagat
      901 ggcaaaatca agagcggaca ataagtatgt taaagagttt gcaaagacga tggtagatag
      961 tcataagcag agcgagaaag acgtcaaaga tgtggccagc aaggctaaga taaaagctgt
     1021 agaaactgac gccagcaaga atcttgaacg ggatattaaa gcgaaggaaa aagaccttaa
     1081 aaaactcaaa ggcgctgaat ttgataaagc ctatattgac caacaaatca ccctgcatca
     1141 acagttgttg gatgacttga actcaaaatt tattcctgca gctcaaagcc cagagttgaa
     1201 ggtatacctg gaaacgacca aggcccatgt ccaagagcac ctttcaaaag ctcgagagat
     1261 ccatacaagc ttgacaaagt aattttttaa aattctagag ggggcattag agagttgctg
     1321 aaggagtccc ccttaaaagt tgcttttatc aggggggaat aatagctatg aattttttaa
     1381 aaaaattaat cagaaccgaa gatgacttgg ttttttttat tttacgattc gttttagggg
     1441 tggtcttctt tcctcacggt gctcaaaagg tcttgggatg gttcggggga ggcggtttca
     1501 cggggactat gggtttcttt accagccaag gtattccggc attttttgcc ttgatggcta
     1561 ttcttgcgga gtttgctggt tccctgggat taattctagg acttctgact cgagtggcgg
     1621 cctttggaat cacttgtgtg atgatggttg ccattttcac tgtccatttg cctaatggct
     1681 tcttcatgaa ttggacagga aaacaagcag gcgaaggatt tgagtatcat ttattggtgt
     1741 tagccataag ttcggtcctc atgtggaaag gagcgggagc ttattcttta gacagaaaat
     1801 tatcttcgtg attccatagt tcagatacct gtcgcttgta cgaaagttga tttatccgtt
     1861 gtcgaattaa tgctaagttg gccattggca ttatttccac aaccaaaaat taatccatcc
     1921 gctctcaatg ctatagtgta gtctttccca cacgcgatcg ccacggcatt ggaaatacct
     1981 gaggcttgta cataacttga tttgttgacc gttgaattga ttccaagctg acctttgtta
     2041 ttatacccac aggtgaaaac taaaccatct gcacgcaagg ctgctgcgtg atcgtatcca
     2101 caagcaatcg ccaccgcatt agaaatacct aatgcttgca caaaagttga tttgctggcg
     2161 gtcgaagccg aattgtctcc aagctgaccc ttggaattca caccacaagc gaagactaaa
     2221 ccatctgaac gtaacgctac tgtgaagttt cccacactaa ttccaatcgt cactgcgttt
     2281 gaaatgccta cggcctgcgt gaaagtgagt ttgctagcgg ttgaattgac gcccagctgg
     2341 ccaccggcgt tgtctccaca agcaaagacc aatccatcgg agcgaatggc agcggtcgta
     2401 tttcttccac aagcaatcgc tgcagcgttt gaaacaccta tggcttgtac aaaagtgtgt
     2461 tgatccacta tggtattatt cgcaagctga cctgtcccac cgctccccga ggtgaaaacc
     2521 aatccatctg atcgaagagc agctgtatga tgagatccgc aggatatcgc caccactctg
     2581 gaaactccaa taccctgcac aaaggtcgat ctatcaaggg tggtgttgtc tccaatagga
     2641 ccgctggagc tattgtaacc acagccaaaa actagaccat ctgcacgcag agcgattgtg
     2701 aaatccaatc cgcaggccaa cgccactgca ttcgaaatac cagtcgcttg tacaaacgtt
     2761 gacttggcaa ctgttgtatt gtcccccaat tggccctttg tgttcgttcc agaagaaaaa
     2821 accaacccat ctgatctcaa tgtagctata tgataattgt ctccgcaggc gattgtagga
     2881 agcttgcgta aggttcctcg tggggttaaa cgggggccct tgatggtaac aatagatcct
     2941 agtttttgcc atgtctgagc taatgcgggg tgaaggttcc caaaaaaaga ttccactgct
     3001 ccagctatgc ctgcaaattt taaaaatttt ctgcggtcca ttgattaagt tcacccttga
     3061 tagtgtctat attgttaata tctaaatatt cttggtaaat ttagtatgaa atacaatctt
     3121 atttaggaaa aattgagaat aataaaggtc attttacttc tgaggtgatc tatccaaaat
     3181 atttaaaaga atttgcctgg tcttaggact tgaaaacccc tgaaaagcct atgtatcttt
     3241 catagaattc cgataactat tcatgcgctt tataattctt ttaatcattt ttatgagttc
     3301 tgcctgtgct actaaaatcc gtcagccaaa tgcagtggat gctcctgcaa atagtttaga
     3361 attggagcag gccctattac aaggtgctgc acccactctc gcatttttga aaacacaaac
     3421 ttctttgaaa agttttgaag gtttaaactc ccgggatcct gcaatttata ttccgaaaac
     3481 tttaaaagcc gaaaaatcct ttccaatctc caaagagaag aacttacagc agcatttttt
     3541 gcgcagattc cgtggttgga atttaaagca aaaaattaaa agagagcatg aactcttagc
     3601 tgactttaat tgcggaaagg ctgttgaaac ccaagccctt ggcttttcct ttgaattgga
     3661 ttttcctgag gccgaagcca gtgctgggtc ccgggccctg catgagcagg tgttatcctg
     3721 cgctactttt ccaagacaag aaagcctttt tcgcttagct gttttctcga ttcacggagg
     3781 agagtgtccc caagccatga aatatcttga tatgtttcca gccactccag agcggggagt
     3841 cagcgatcga atttcttatt tgcgcagtct ttgctcaact ttccctatcg caaaaaatgg
     3901 aaatccttgg ggcggttacg gcagccttct tggtgatatt aagggggggg aagagccaag
     3961 ttctccaaaa cccaagtggt atctatccac atccagtggt tctgaagagt gggaccgttt
     4021 gctggtctca tttatagagt tgagtgaaaa aaatgaaggt gccaccgttc agtatatttc
     4081 gagtaaactg aattttgaaa aattccgcag tctgcccttg gccttccaaa cttcgatgtt
     4141 agtgatgatg agttttaatg gcgcggatct ttctgtattt caaaccctgc accgttatct
     4201 ttcagatcat ccagaaacga tttcacctgc cgtggccggg cttctctttc ccattcgcta
     4261 ttggaaagaa attatagcca atagcaagag tgcggatccg attttagtca aagcgctcat
     4321 tcgccaagaa agtgccttta atccgaccgc gcgcagtcgg gcaaaagctg ccggtcttat
     4381 gcagttgatt tatccgaccg caaagttttt tggaatcacc caaccaaaac aactcctggc
     4441 ccctgaagca aatattcatg cgggctcaga atttttaagt cagttgatta atcagtttgg
     4501 atctgtggag ttagcattgg cagcttataa tgccggtcca gcgactgttc gagactggca
     4561 aagacgttat ccaaccaata atatagattt atttgttgaa atgattccct attcggaaac
     4621 gcgcgaatat gttcgtttag tgaggcgcaa ttacaaagtt tatcaatcca ttttaatgaa
     4681 gacgcaatat ataggggact taagctcggt tgggacagag tgattgccga ttccttgtga
     4741 attatcagaa atattggcca ttttttataa gacacacaca tctttaattg gagaaatgat
     4801 ctttctacaa cgagttcttg aacaatggtt tttgcaatcg aggagctttt tgcgataata
     4861 gcttcatgga ctgggaaaaa gagtgccctg cactttgcca tttaattgaa aa
//
LOCUS       DATBTE010000300         5678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2156429, whole genome shotgun sequence.
ACCESSION   DATBTE010000300 DATBTE010000000
VERSION     DATBTE010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2156429"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VIG33_11085"
     CDS             complement(<1..229)
                     /locus_tag="VIG33_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271422.1"
                     /translation="MNQNFTRQFLFSYALSVVVAGSLLVNPVPSLAQSKAAIASNAAE
                     KVYVAPGKYDEFYSFVSGGFSGQVSVYGLPSG"
     gene            518..991
                     /locus_tag="VIG33_11090"
     CDS             518..991
                     /locus_tag="VIG33_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8271423.1"
                     /translation="MMKLTNVLVAVVAITAMEMSAPVLAETGASKGIGPMTEIKLESL
                     SESMAKQGKEVFNAKCSMCHKMEERYVGPALNDVFKRRTPEWIGNIILNPIEMTQKDP
                     AAQELLAEYLTQMTFQNISNEQARQIIEYFRYYSEKGDLTEPKVEAKSKAKSKTK"
     gene            1002..1550
                     /locus_tag="VIG33_11095"
     CDS             1002..1550
                     /locus_tag="VIG33_11095"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEY8271424.1"
                     /translation="MTKKIVLILALSVLSAQSFAEKPKSKSTPPHAESKLKLELIKLN
                     GSEGKDIVEIATGSKDHSTLVAALKAADLVDTLTGPGPYTIFAPVNSAFDKLPKGTVE
                     SLLKPENKLKLKSILEHHAAAPAYNPTILSEMTEVDMVDGPKLQVKVKDQKIFVDDVE
                     IKTAISAKNGIVYIVDTVLTGK"
     gene            complement(1625..2680)
                     /locus_tag="VIG33_11100"
     CDS             complement(1625..2680)
                     /locus_tag="VIG33_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEY8271425.1"
                     /translation="MSKRFSRQIRLSEVGERGQDAIKKSRILIVGMGGLGCPAAQYLV
                     AAGVGCLGIIDGDRVEETNLHRQILYFDEDIGRAKVEVAAARLQALNPSTEINYYFAP
                     LDSENSNPILQSYDIILDCTDNFEVKYLLNDVCLRMKKTLVSASATGFEASLMVIEEG
                     GPCLRCLYPQVNSDDIGNCNIMGVLGAFVGIVGSWQAAETLKILLSRTEQQRSFLHTK
                     GKVLFFDFYTSQIRSVALRKNPDCFCVHQNCNRPEVKKESLYLTADQIGLLRDYVLID
                     VRSVEEREHESFEGFGGGEVIHIPYQEIISNEFDSEFWRGGKKYVFFCSMGKRSTAAA
                     QWLRDQGVQSAYSLRGK"
     gene            complement(2677..3021)
                     /locus_tag="VIG33_11105"
     CDS             complement(2677..3021)
                     /locus_tag="VIG33_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271426.1"
                     /translation="MNIRADSKEVKFRLDQEEVGVLLKEGEVLEKIRFPASQICFRIK
                     FGEQTRVEQIDDEVLFYLALGERDKFQRLFEDKLGIKDPIVVVCQEIKSQKVSFKLEV
                     DIFKPKQREQRK"
     gene            complement(3005..3610)
                     /locus_tag="VIG33_11110"
     CDS             complement(3005..3610)
                     /locus_tag="VIG33_11110"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEY8271427.1"
                     /translation="MAQGTNTQPDLRGVFGVVLAGGKSSRMGSDKALLSFKGGTLLEH
                     QFKKLSSLLGVENVIVSGNYPPLRHRADETPELGPLGGISSLVREFPLAEFLFFLPVD
                     MPNLSEKTLLRLLQAAQRDFSHNSWSLSHFEMPLILRNNAELKLILKDLILQPRNFRS
                     VRRLCELLKHTAVDTTDILSTEFLNTNTLKEWEEVIDEHTR"
     gene            complement(3577..4035)
                     /locus_tag="VIG33_11115"
     CDS             complement(3577..4035)
                     /locus_tag="VIG33_11115"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY8271428.1"
                     /translation="MGRFNPMGKILVAMTESVLDVQKAIAHCRSEDCGAEILFIGTVR
                     NINEGRAVSAVNYDGHKVLGEKILLELCLEAQAQWGDNMNFWVEHRLGVLKVGEESLL
                     IHVTSPHRDSAYQASRYLIEQIKLRLPVWKEEIYWEGDTEWLKGQILNQI"
     gene            complement(4037..4297)
                     /locus_tag="VIG33_11120"
     CDS             complement(4037..4297)
                     /locus_tag="VIG33_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271429.1"
                     /translation="MINVNISLYGAFRRLGVQQLSLEISDESTVADLRDCMAKYLENI
                     DANISVHLVKTSAFATDERVLKEEDRVAGLKCLAILPPVCGG"
     gene            complement(4294..5571)
                     /gene="glp"
                     /locus_tag="VIG33_11125"
     CDS             complement(4294..5571)
                     /gene="glp"
                     /locus_tag="VIG33_11125"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY8271430.1"
                     /translation="MIQFEEAIARIASVARQYSPESEVIPLEEADGRICTEDILSPEA
                     VPSFNNSAMDGFAICSEDTLGASEENPVRLSVSSIAAAGDEVVSYSSDVRAIEIMTGA
                     ALPDIFDAVVKIEDVKILRSEAGGGHGILISSFIPKGNNVREKGEDYKVGQIVLSHGA
                     CIRAESMMALAGLGISEVTVFKKPVVSILSTGKELVPLETKSLKPGRIRNSTALYLKT
                     AMAHLGARIHSVETIGDEVGVFKDHVQRNLAQNIDVLVTTGAVSMGKYDFIVPALLEM
                     GAEIVFHKVAIRPGKPILFAKIKRSRGNPLIIFGMPGNPIASAVGFRFFLTPFFREFY
                     GQHPEQAWSVKLSETTRKPENLKCFFKARLEKEAGDLVVRSLAGQASFMISPLVQANA
                     WVLFPEVGTQVSKNQRVDVFPLLQNEFFEWGNL"
BASE COUNT         1657 a         1394 c         1075 g         1552 t
ORIGIN      
        1 ttccggaagg caatccgtag acagaaacct gtccactaaa accacccgaa acaaatgaat
       61 agaattcatc atattttcct ggcgccacat atactttctc tgcagcattc gaagcgatag
      121 ccgctttact ttgcgccaag gatggcactg ggtttactag taagcttccg gcgacaacca
      181 cactgagggc gtagctgaag aggaactgtc ttgtaaaatt ctgattcaca ttacctccaa
      241 taataaaagt ttctggcatt gattcattct taaagaatgc cctagtctat agccctactt
      301 tcaaataatt caagctatta atgtcagaga caaaatgtct cacttaggtt ttcggcatag
      361 acagagtgtc tcaagatatt ttgcccccat ttgtcttcaa gccaaagtcg ttgaatttaa
      421 aatataagtg aaaatatcat agacatgttt ttcatttggc ctgattatca acgtcgaagc
      481 ttgatgtgac gactgacttt ggttgtcaga aaggtaaatg atgaaactca ctaacgtact
      541 tgtcgctgtt gttgccatta ccgcaatgga aatgtccgcc cctgtcttgg ctgagactgg
      601 cgcgagcaaa ggaatcggcc ctatgactga gatcaaactt gaaagtctta gcgaatccat
      661 ggccaaacaa ggcaaagaag tttttaatgc taaatgctct atgtgccaca agatggaaga
      721 acgctacgta ggaccagcat taaatgatgt tttcaagaga cgcactcccg aatggattgg
      781 aaatataatt ttaaacccca tcgagatgac ccaaaaagat ccagcggccc aggaactttt
      841 agcggagtat ttaactcaaa tgacgtttca aaatataagt aacgagcaag ctcgtcaaat
      901 tatagaatat ttccgctact attctgagaa gggcgatctc acagagccaa aggttgaagc
      961 aaagtcaaaa gcaaagtcaa aaacaaaata ggagaacact tatgacgaaa aaaatagttc
     1021 ttattctcgc acttagcgtg ctttccgcgc agtcttttgc ggaaaaacct aagagcaaat
     1081 ccacacctcc gcatgcggaa tccaaattga agcttgagct catcaaattg aatggctctg
     1141 aaggaaaaga tattgttgaa atcgctaccg gctcaaagga ccactcgacc ttagtggccg
     1201 cattgaaggc tgctgactta gtggatactt taactggccc tggtccttac actatttttg
     1261 caccggttaa tagcgctttt gacaaattac caaaaggcac cgttgaaagc ttgctaaagc
     1321 ctgaaaataa attaaagctc aaatcaattc ttgagcatca cgctgcggct ccggcttaca
     1381 atcctaccat attgtccgag atgaccgagg tggatatggt cgacgggccg aagcttcaag
     1441 taaaagtcaa agaccaaaaa atattcgttg atgacgtcga aattaaaacg gccatttcgg
     1501 ctaagaatgg gattgtttac attgtcgata ctgttttgac tggaaagtga ttctctttct
     1561 ttttagaaat tatattggca gatcagagga taaactctcg tctgccgctc taaaaaattc
     1621 ccattcactt tcctcttaga ctataagcac tttggacacc ctgatcccgc agccattgcg
     1681 cagcggctgt ggatcttttt cccatgctac aaaaaaagac atatttctta ccccccctcc
     1741 aaaattcaga atcaaattca ttagaaatga tttcctgata aggaatatgg ataacttcac
     1801 ctcccccaaa accctcgaat gactcatgct ctctttcttc tactgaacgt acgtcgataa
     1861 gaacgtaatc tctaaggagt cctatttgat cggccgttag ataaaggctt tcttttttaa
     1921 cttccggccg attgcagttt tgatgaacac aaaagcaatc tggatttttt cttaaagcga
     1981 cagatcgaat ctgagaagtg taaaaatcaa aaaataaaac ttttccctta gtatgcaaaa
     2041 agcttctctg ttgttctgtt cgactcagga ggatctttaa agtttcagcg gcctgccacg
     2101 aaccaacgat tcccacaaag gcgcccaaaa cacccattat gttacagttt cctatatcgt
     2161 cagaattgac ctgtggataa agacaacgaa gacagggacc gccctcctcg ataaccatta
     2221 aactggcttc aaagcctgtg gcggaggcgg ataccaatgt ttttttcatg cgtaagcata
     2281 cgtcattcag taagtacttc acttcaaaat tatccgtaca atcgagaatg atgtcataac
     2341 tttgcaaaat gggattcgaa ttctcagagt caaggggtgc gaaataataa ttaatttccg
     2401 tgctgggatt taatgcttgc aatcgggccg ccgccacttc gacttttgct cttcctatgt
     2461 cttcatcaaa gtatagaatc tggcgatgca gatttgtttc ttcaacgcgg tcgccatcaa
     2521 taattcctaa acatcccact ccggcagcca ctaaatactg agccgcagga caacctaatc
     2581 cccccatccc gacaatgaga atcctggatt tttttattgc gtcttgtcct ctttccccaa
     2641 cttcagagag tcggatctga cgtgagaatc gcttgctcat tttctttgct ccctttgctt
     2701 tggcttaaaa atgtccactt ctaatttaaa ggaaactttt tgacttttga tctcttggca
     2761 aaccacgact ataggatctt tgatgccgag tttatcttca aaaagtcgct gaaatttatc
     2821 acgctcccct aaggctaagt aaaataggac ctcatcatca atctgctcca ctcgcgtttg
     2881 ctcgccaaat ttgattcgga aacatatttg actggctgga aagcgaatct tctccaaaac
     2941 ttctccctct ttcagaagga cacccacttc ctcttgatca agtcggaatt tcacttcttt
     3001 tgaatcagcg cgtatgttca tcgatgacct cttcccattc cttgagggta ttcgtattta
     3061 aaaattcagt actaaggata tcagtggtat caacggctgt gtgtttgagg agttcgcaca
     3121 gtctgcgtac agaacgaaaa ttcctgggtt gcaaaatgag atcttttaaa atcaatttta
     3181 attccgcatt atttctcaga atcagaggca tttcaaaatg actcaatgac cagctgttat
     3241 gtgaaaagtc cctttgagcg gcttggagaa gacgaagaag tgttttttca cttagatttg
     3301 gcatatccac tggtaaaaag aaaaggaact ccgccaaagg gaactctcgc actaaagatg
     3361 agatcccccc taaagggcct aattcaggtg tctcatccgc tctatgcctc agcggcggat
     3421 aattgccgct cacaataaca ttctcaacac ccagaagaga acttaatttt ttaaattggt
     3481 gctcaagtaa ggttccgccc ttaaaactca gtaatgcttt atctgatccc atgcgcgagg
     3541 actttccgcc cgccagcacg acaccaaaaa caccccttaa atctggttga gtatttgtcc
     3601 cttgagccat tctgtatcgc cttcccaata aatttcttcc ttccaaaccg gcaatctcaa
     3661 tttgatctgt tcaatcagat agcgcgaagc ctggtaagcg gaatcgcgat ggggagaagt
     3721 cacgtggatc aataaacttt cctcgcccac cttcaaaact cccaatctgt gctccaccca
     3781 aaaattcata ttgtctcccc attgagcctg cgcctccaag caaagctcca gcagtatttt
     3841 ttcgcctaat actttgtgcc catcataatt caccgcgctt acggccctgc cttcattgat
     3901 atttctgaca gtgccaataa ataaaatttc cgctccgcag tcttcactac ggcaatgggc
     3961 gatggctttc tggacatcga gcacactttc agtcatggcc actaaaatct ttcccatcgg
     4021 attgaatctt cccattctag cctccgcaaa ccggaggtag gattgccaga cactttaaac
     4081 ccgccactcg atcctcctct tttaaaacac gctcatccgt tgcaaaggcg gaggttttaa
     4141 caaggtggac agatatgttc gcgtctatgt tttccagata ttttgccata cagtcacgta
     4201 ggtcagccac tgtggactca tctgatatct ctaaactgag ttgctgaact ccaagccgcc
     4261 ggaaggcgcc atataaacta atattcacat ttatcataag tttccccatt caaagaattc
     4321 attttgtaaa agaggaaata catccaccct ttgattttta cttacctgtg ttcccacttc
     4381 aggaaacagg acccaggcat tggcctgtac caaggggctt atcatgaacg aagcttgccc
     4441 cgctaaggaa cgcaccacaa gatcacccgc ctctttctca agacgcgctt tgaaaaaaca
     4501 ttttaagttt tccggcttgc gcgtggtttc tgaaagcttg actgaccaag cttgctctgg
     4561 gtgctgccca tagaactcgc gaaaaaaagg agttagaaaa aaacgaaatc ccaccgccga
     4621 agcaatagga tttcctggca taccaaaaat gattaaagga tttcctctgc ttcttttaat
     4681 tttagcaaag agaatcggtt tgcccggacg aatagccact ttgtggaaaa ctatttctgc
     4741 ccccatttcc agaagagcgg gaacgataaa atcatacttg cccatggaaa cagctcctgt
     4801 cgttactaga acatcaatat tttgagcaag attccgctga acatgatctt taaaaactcc
     4861 cacttcatcc ccgatggttt ctacagagtg gatccgagcc cccagatgag ccatcgccgt
     4921 tttcaaataa agagctgtcg aatttcgaat ccttccgggt ttcaaactct ttgtttcaag
     4981 cggaaccaac tcttttcctg ttgaaagaat cgaaaccacc ggctttttaa aaaccgtgac
     5041 ttctgaaatt cccaatccgg caagcgccat catggactct gctctgatac aggcgccgtg
     5101 tgataaaaca atttgaccga ccttataatc ttctcccttt tcacggacat tgtttccttt
     5161 cggaataaat gagctgatga gaattccatg accaccaccc gcttcagagc gcaggatttt
     5221 aacatcctca atcttgacaa ccgcatcgaa aatatctggc aaagctgcac cggtcataat
     5281 ctcaatggcg cgaacatcag atgaataaga gacaacctca tctccggccg cagctatgga
     5341 cgaaaccgat aagcgcacag gattctcttc agaagcccct aaggtgtctt ccgaacaaat
     5401 ggcaaagcca tccatagccg aattattaaa ggaaggaacc gcctcggggc ttaaaatatc
     5461 ttctgtacat atacgaccat ccgcttcttc cagcggtata acctcagact ccggtgaata
     5521 ctggcgcgcc acactcgcaa tgcgtgcgat cgcctcttca aattgtatca tgttgccttc
     5581 tttccaaaaa taaaaggacc cctttagtga aaacttcaaa tattatcccc acacccagca
     5641 cccacaacat gttgccagag aacaatgccg ccaagccc
//
LOCUS       DATBTE010000301         8810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1746842, whole genome shotgun sequence.
ACCESSION   DATBTE010000301 DATBTE010000000
VERSION     DATBTE010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746842"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VIG33_11130"
     CDS             <1..488
                     /locus_tag="VIG33_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271431.1"
                     /translation="YVILESQPQNKIRDRQKDGSIKRKPLKGKPRIDSKHNRLIPIIS
                     KELFNNLVRLYKIQQAKFEKKVYGPNPKDYVLFEDLTHSETVVALREAYAKTKYAQKS
                     YHCCRHTRCTELVGKTRDFVLARYWLGHARQETTLRYTHIYQQSANTARKKIQKIDFV
                     D"
     gene            1068..1664
                     /locus_tag="VIG33_11135"
     CDS             1068..1664
                     /locus_tag="VIG33_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271432.1"
                     /translation="MKEIEEHPDWYYPVQDMRTLILGTFPPHQKQWDYPFYYPNRANH
                     FWKILAEVGKFTLTQWEGMPAVEERKFLMRQMRVGVQNIGLVVERENQSALDKDISVL
                     KFQDLLSIINSNDSLQKVLLTGYSGKTSTYREFTKYLKMMGISYTKPDKVGAGFEFTA
                     QFNRPINCLIGNSTSPTAQRGGISFEMLVDQFRKAITA"
     gene            1868..5866
                     /locus_tag="VIG33_11140"
     CDS             1868..5866
                     /locus_tag="VIG33_11140"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8271433.1"
                     /translation="MPNKLGSHLRNLRNHLKKTLRQVEAESGISNAHLSQVEKGAIEK
                     PSTELLKKLSDYYQVELSYLKSLAGYSEDKYPAKEDSKILPLKYLTAAQLNTFAGIKE
                     SEGILPELVRDLVVSTINLDHLMMPVGNSVFKHGWDGLLNCVEKPSVQFVPQGFSCWE
                     FSRAKDVTTKAEKDFDKRTQKPLGQDVENSTYVQVSFRQVGDAEKNKFIAEAKTKSKW
                     KDITFLDADDLELWLERSPVTALTYARRLGLFPNTPGYRPLSETWDQWWCSTNPPIDE
                     SILLEAGKRCDDVKQFTADSSCEHLKIKTNNTDVGIAFLYASFATMEDKTIGQSLISR
                     SMVVDTLDAFRLLATRGKNLILIPTFKGAPIGEAKFNGHRIVSIVNNFDPHDVDSVGR
                     VRTAVIGDFLTAEGGFPNEKAYRMAQACYGNLSVLKLMLADEGEAAQPTWARPDFAVS
                     TLPLAMLGGWNSGNEADKEIVKKIAQQEFDSVETYLSSIPENNELLFQKVENHLSFYQ
                     EYCLGHLRSFLTEKSLENFFAVAKEVLCQINTAYDLDIDKRYAAHIYKKGNKYSSWLR
                     KGITTTMAMLATMDHPSFPHLQQRVDHLVNEILGDKTDWKFWASLNDIMPLLAEASPE
                     AFLGKVQSASLKDDFVEILNQSATMGGCEYAALLWSLELCAWDRRYFSQVSNILLTLA
                     TKENTKSNYVNKPSNSLWDIYRIWANQSTIPFKERIEILKSLEKAHPETSWKLFLDIV
                     PTPHESGSYTNRPKFREIDEDPNKDSGLAYVSALAIIIDHVLTVVDSNLHKWFDLIDL
                     SFKIRPRSFQTKILEKLTSTNLDKFSLEDKMAVWRKLEKELAHHMDFPKSEWSLKGAN
                     LALVKKAYKKFEPKDLMKKWEHIFDYSLSHPEIEGGDDYKKKNENLRALQKKALKDLI
                     PFEKNIESLIPLIKAAPNSWILGTIFAEVHPDLTVAAIKIANNEERGADFVSGAVFTL
                     FKNNGIDWLEKLLSNPISTETKLRLQIFLSLPTEGATWERLSLENKELQTSYWKSVKR
                     FFVMDNLQDASYAIAKFLENNRPVEAFYVLAYFERHKDESIASNAAMEVLDAIVSCDL
                     NEEAGSVDFNLFDYHLENILNELGKRDDVDKRKLAMIEWNFLPIFKYRKKPEHLSAEV
                     NNDPTLFIDILKLAYKKDDYTDEGDGTEAAKSRAWDFLHDNKTLPGIAKDGFNSEQFQ
                     KWFLDARALAIQCNRLKSFESVMGQLFAYSPFDPDDGGWPHRAVRDVTEEFGSDEMAN
                     GFSVGIFNKRGVFHRAYGEGGTQERELAKRYGEYAKICEISWPKTAKVLRKMEAGYSR
                     DAHREDVRALHQ"
     gene            complement(5881..7743)
                     /locus_tag="VIG33_11145"
     CDS             complement(5881..7743)
                     /locus_tag="VIG33_11145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiV family abortive infection protein"
                     /protein_id="HEY8271434.1"
                     /translation="MRIYLKVLAALYFAGFIFHAADLFDARLRFSEMDSIWKTWILFL
                     AAADLAAAVGLWRGKRFGEILFVLVAVCQLFAYLCFKSFFGNQTFLVIFHFVTLAVYF
                     GFRGLHEYKLRSGPRSLLSSADEKQNDAVENAKRFLTCAKESEQIAPNVAYVLATTAL
                     EEVGKSLLKKQKLLEKYSGKPNPSWLAKHEDDHQKKLFWAIWTPMLGNKVITGKDFVE
                     SKELASSIHNKRLRAQYSDDGHSTQLAVNPEEVRNLIQFTDARIKIEESYKPIDPDPE
                     RVEKMRWFLGIHEAREDSKLIFGGRSMQKLSELGDVYLWVCWLKDQFDEADQDAIRLS
                     ESELNREIDKTAEKKIKWKMTVKLVSKSHSIRPGEFAKWNETVEQIKIRAGDPKKREI
                     LVDFFLPEQFHIKTLWWQSHALVNHFLIALNIGTFGYFWWYVPTITERFYEKLEDLEN
                     KSEVRIENHPNKPIVWGNLALKKDDLNRVAICLPFIYRSKETKIFDHYLTGLGFLAKT
                     DVVMRFDANALEAFARALQLIVEKYREIPLIDFLARPTEAIGAICSDWKEEDRNRITE
                     LVGWAFSDKRNTLAIEQEDVGIMKILTDNILQELARNEAREHFSGKKSEAPSNS"
     gene            complement(7753..>8810)
                     /locus_tag="VIG33_11150"
     CDS             complement(7753..>8810)
                     /locus_tag="VIG33_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8271435.1"
                     /translation="VHIKSWQEAYNGLVPQDYLDALPGEFESRVKMWTSILANPKRWA
                     WVAEGQNGIVGFVLFGPPRDPNREGFIELGAIYLLASEKGNGIGFSLLSAGFNKMRDL
                     GYKRAYCWVLENNPTIKFYERSGATFSSQTKEDEIGGKNFKELAYDWDSLNIGDYNWD
                     PLSVDEVKNVFKPFKHQWWIAGGWAIDLFLGKQTRKHDDIDVLILREDQLEIQNLLAG
                     WDLWAADPPGTLKPWPKGEILGKGLQDIWGRRTPKYPWQIQIMLFDTENEEWIFKRDE
                     SIRRRLSSITVTTNDGTSLLAPEIQLLYKSKSLRSKDQQDFENAITVLSDDQKNWLKD
                     ALIKIYNGGHEWLKKMD"
BASE COUNT         2641 a         1925 c         1809 g         2435 t
ORIGIN      
        1 gttatgtgat ccttgaaagc cagcctcaga acaagattcg agatcgtcag aaagacggat
       61 cgatcaaaag aaagcctcta aaaggaaaac cacgaattga ttcgaaacac aatcgcctga
      121 tacccatcat cagtaaggag ctctttaata atttggtgcg cctgtataaa atccagcaag
      181 cgaaatttga gaaaaaggtc tatggcccaa atcccaaaga ttacgtgctc tttgaggatc
      241 taactcactc agaaacggta gttgctctaa gggaagcata tgcaaaaacc aaatatgctc
      301 agaaaagcta ccactgctgc cgccatacgc gttgtactga attagtaggt aagaccaggg
      361 atttcgtttt agcacgttac tggctaggcc acgcacgcca ggaaacgacc ctgcggtaca
      421 cgcacatcta ccagcagtca gcaaatacag ctcgaaagaa aatccaaaaa atagatttcg
      481 tggattgaga ggattgaaat gaaatatgaa aatgatgaaa ttttagtggc taatgtcggt
      541 ggtagataca aaggccttct taacttctcg attttgaatt cctggattct cacaatagat
      601 tgcacattca gagtaggaga tatgtttgaa ctaccctttg attattttcg aagatttgag
      661 ctaaattcag agtttaacta acacgacacg agcgatccga cttcaattta tagattcaga
      721 ttacttcgcc tcgaaggtga ttctgccatt cttagaattc agaaaatctt ggaagagcaa
      781 ggcgacatgt ggttgtatga aaatgcaatt aagtatggga ctgactttgg aattatcgag
      841 ttcgtaggtg ttcaaagaaa gcctgatctt aaattgatta aatttggata ctagatttcc
      901 ttagcgagtc attggccgca aaaaaccttg cgatatgccc aaatctttta ataccacggc
      961 aaggaaggcc tagaagattg agtttaacga acgaaaggag aataatatta aggaactaaa
     1021 gtcagatccc tgccagtagc caaaatttat tcttaaggga ggagcgcgtg aaagaaatag
     1081 aggaacatcc tgattggtac tacccagtcc aagatatgcg tactttaatc ctcgggacct
     1141 ttccacctca tcaaaagcaa tgggactacc cattttacta tccgaatcgc gcaaatcact
     1201 tttggaagat tttagccgaa gttggaaagt tcacattaac gcaatgggaa ggaatgcctg
     1261 ccgtagagga gcgtaaattt ttgatgagac aaatgcgggt tggcgtccag aatattggcc
     1321 ttgttgttga acgcgagaat caaagcgccc tagataagga catttctgtc ttaaagtttc
     1381 aagacctgct cagcataatt aatagtaacg actctcttca gaaagtgctt ctcacaggat
     1441 attctggaaa aaccagtaca tatcgtgaat ttacaaaata tttgaaaatg atgggaatta
     1501 gctacaccaa gcctgacaag gtcggcgccg gctttgaatt taccgcgcaa ttcaaccgac
     1561 ctatcaactg cctcatcggc aattcgactt cgcctactgc ccagagaggt gggataagtt
     1621 ttgaaatgct cgttgatcaa tttcgaaaag caatcaccgc ttgaccgcac ttcagtccgt
     1681 gacaaaaaaa tcaaaaatat aagtcatggc ctcgcttagg gaggattagc atggcccctc
     1741 cttttgccgc catggataaa aatcttatct aattgatttt atttgtattt atcaacggca
     1801 attggaccta tttacaattt tgctaattct tgctaatctc aatttattcg tttagcaagg
     1861 agtcactgtg cccaataagc taggatcaca cctaagaaat cttcgaaacc accttaaaaa
     1921 aaccctccga caagtggaag ccgaatctgg aatcagcaat gctcacctta gtcaggtaga
     1981 aaagggtgca atcgagaagc cctcaaccga gcttttgaaa aaactctccg attactacca
     2041 agttgaactt agctatctaa aatctttagc tggatattct gaagataagt atcccgcgaa
     2101 agaagattca aaaattcttc ctctaaaata tctgacggct gctcaactaa acacatttgc
     2161 aggaatcaaa gaatctgagg gcatacttcc tgagttagtg cgtgaccttg ttgtatctac
     2221 gatcaacctt gatcatctta tgatgccggt aggcaacagc gtcttcaaac atggatggga
     2281 tggacttctt aattgtgtgg aaaaaccatc cgttcaattt gttccgcagg gcttttcatg
     2341 ttgggaattt tcgcgagcta aagacgtcac caccaaagca gagaaagatt tcgataaacg
     2401 aactcaaaag ccacttggcc aagatgtgga aaactcaact tacgtgcaag tttcgttcag
     2461 acaagttggt gatgccgaaa aaaataagtt cattgcagaa gccaaaacaa aatcgaaatg
     2521 gaaggacatt actttcctgg atgcagacga tcttgaactc tggcttgaac gtagtccggt
     2581 gaccgccctc acctacgctc gtcgactggg gttgtttccc aatactccag gatatcgccc
     2641 actcagcgaa acttgggacc aatggtggtg ctcaaccaat ccaccaatcg acgaaagtat
     2701 tttactcgaa gcgggaaagc gttgtgacga cgtaaagcaa ttcaccgcag actcttcttg
     2761 tgagcatttg aagatcaaga caaacaacac tgatgttggg attgcatttc tatatgcttc
     2821 ctttgcaact atggaagata aaaccatcgg tcaaagttta atttctcgct caatggtcgt
     2881 cgacactctt gacgcctttc gcctgctagc aactcgcggg aaaaacctaa ttttaattcc
     2941 gactttcaaa ggtgcgccca tcggtgaggc caaatttaac gggcatcgta tagtaagtat
     3001 cgttaataat tttgatccac acgatgttga ttcggtagga agagtaagaa ctgccgtcat
     3061 tggagatttt cttacagcag aaggaggttt tccgaacgaa aaggcatatc gtatggcaca
     3121 agcttgttac ggcaatctgt ctgtactgaa attgatgctc gccgatgaag gtgaggctgc
     3181 acaaccaaca tgggcaaggc ccgactttgc ggtctcaaca cttccattgg caatgctcgg
     3241 tggatggaac tcaggaaacg aagccgacaa agaaattgta aaaaagattg ctcagcaaga
     3301 gtttgactct gttgaaacct acctctcgtc gattcccgaa aacaatgaat tactattcca
     3361 aaaagtagaa aatcatcttt ctttttacca agagtattgc ttgggacacc tacggtcatt
     3421 tctaacggag aagtcattag aaaacttctt tgcagttgcc aaagaagtac tttgccagat
     3481 caacactgca tacgatctcg atattgataa acgctacgct gctcatattt ataagaaagg
     3541 taacaagtat tcatcatggc taagaaaagg gattacgact actatggcta tgcttgctac
     3601 gatggatcac ccttcgtttc cacacttgca gcaacgcgtg gaccacttag tcaacgagat
     3661 cctgggcgat aagacagact ggaagttttg ggcgagctta aacgatatca tgccacttct
     3721 tgcggaagca tctccagaag catttctagg aaaagttcaa agcgcatcgc ttaaagacga
     3781 ctttgtagaa atcctaaacc aatcagccac aatgggtggc tgcgagtatg cagccttgct
     3841 gtggtcttta gagctgtgtg cttgggatag aagatacttt agccaagtca gcaacattct
     3901 tcttactctt gcgacaaaag aaaatactaa gagtaattac gtcaacaaac catcaaactc
     3961 tttgtgggat atctatagaa tctgggcaaa ccaaagcaca atccctttta aggagcgtat
     4021 cgaaattttg aagagtcttg aaaaggctca tcctgaaact tcatggaaat tgtttctaga
     4081 tatagttcca actcctcacg agagcggcag ctataccaat agaccaaagt tccgtgaaat
     4141 cgacgaagat ccgaataagg atagcggact tgcatatgtt agcgctttag ccattattat
     4201 cgaccatgtg cttacggttg tagattcaaa cttgcacaag tggtttgatc ttattgacct
     4261 aagcttcaag attcgaccaa gatcatttca aacgaagatt cttgagaagc ttacttcaac
     4321 taatttggat aaattttcac tcgaagacaa aatggcagtt tggcgtaagc tggaaaaaga
     4381 actcgcccac cacatggact ttcctaaatc agaatggagc ctcaagggag ccaacctcgc
     4441 cttagttaaa aaggcctata aaaaattcga accaaaagat ttaatgaaaa aatgggagca
     4501 catcttcgac tactctcttt ctcatccaga aattgagggt ggtgacgatt ataaaaagaa
     4561 aaatgagaat cttagagctt tgcagaaaaa ggcattgaaa gatttaattc cattcgaaaa
     4621 gaatattgaa tcattgattc cactcattaa ggcagctcca aactcgtgga tactaggaac
     4681 tatatttgca gaagttcatc cagatttgac agtagcagca attaaaattg caaataatga
     4741 agagcgcggt gctgatttcg taagtggcgc agtgttcacc ctcttcaaaa acaacggcat
     4801 cgattggctt gaaaagctgt tatctaatcc gatctctaca gagacaaagc tcagacttca
     4861 aatattcttg tccttaccta cagaaggagc tacgtgggaa cgcttatcac tagaaaacaa
     4921 agaactacaa acttcctatt ggaaatccgt aaaaagattc tttgtgatgg ataacctcca
     4981 ggacgcctct tatgcaattg caaagttctt agaaaacaat cgccctgtag aagcatttta
     5041 tgttcttgcc tattttgagc gacacaaaga tgaaagcatt gcttcgaacg ccgcaatgga
     5101 ggtacttgat gctattgttt cctgtgatct aaatgaggaa gccggtagtg tcgattttaa
     5161 cctgttcgac tatcacctgg aaaatattct taacgaacta ggcaagcgtg atgatgttga
     5221 taaacgtaag cttgcgatga tcgagtggaa ctttctgcct atttttaagt atagaaagaa
     5281 acccgaacat ctatcagccg aggtgaataa tgatccaacg ctatttatcg acattctcaa
     5341 gctcgcgtac aagaaagatg attatacaga tgagggagat ggaactgagg ctgccaaatc
     5401 tcgtgcttgg gactttcttc atgacaataa gaccttgcca ggaatcgcca aagatggatt
     5461 taactctgaa cagtttcaaa aatggtttct ggacgctcga gcacttgcca ttcaatgcaa
     5521 tcgcttaaaa tcctttgaaa gcgtgatggg tcagctcttt gcatattcac catttgatcc
     5581 tgacgatggt ggatggcccc acagggccgt tcgcgatgta actgaagaat ttggatcaga
     5641 tgaaatggct aacggatttt cagttggcat tttcaacaag aggggcgtat ttcaccgcgc
     5701 ctatggtgaa ggcgggactc aggagcgtga gcttgcgaag agatatggag aatatgctaa
     5761 aatttgcgaa atttcttggc ccaagactgc taaggttctt agaaaaatgg aggccggcta
     5821 ttcgcgcgat gcacatcgag aagatgtcag agctttgcac cagtaattat tatgcagaac
     5881 tcaagagttg ctaggcgctt cagatttttt gcctgaaaaa tgttcgcgag cttcgtttct
     5941 agcaagctcc tgcaaaatgt tatcggtaag gatcttcata atacctacgt cttcttgctc
     6001 aattgccaga gtattgcgtt tatctgagaa cgcccagcca acaagttccg ttattcgatt
     6061 tcgatcctcc tctttccaat cgctacaaat tgctccaatg gcttcagtcg gccgagctaa
     6121 gaaatcaatc aatggaatct ctcggtactt ctcgacgatc aattgtagcg ctctggcgaa
     6181 ggcctcgagg gcattagcgt caaagcgcat cactacatct gttttagcga gaaaccctag
     6241 gcctgtgagg taatggtcaa aaattttggt ctctttgctg cggtagataa acggtagaca
     6301 tatcgcgact ctatttaagt catctttctt caatgccaag tttccccaca caattggctt
     6361 attaggatga ttctcgattc gaacttctga tttattctcc agatcctcga gtttttcata
     6421 aaatctttct gtaattgtgg ggacgtacca ccaaaagtaa ccgaacgtac caatgttaag
     6481 agctatcaaa aagtggttca ctagtgcgtg agactgccac caaagcgttt ttatgtgaaa
     6541 ttgctctggc aaaaagaaat ccaccagaat ttctctcttt ttaggatctc cagctcttat
     6601 ttttatttgc tcaactgttt cattccattt ggcaaattcg ccgggccgaa ttgagtgaga
     6661 cttagatacg agctttaccg tcattttcca tttgattttc ttttcagcgg ttttgtctat
     6721 ttctctattc agttcagatt ccgacaaacg tatcgcgtct tgatctgcct catcgaattg
     6781 atcttttagc cagcagaccc acagatagac atcgccgagt tcagacaact tttgcatgga
     6841 acggcccccg aagataagtt tactatcctc ccgcgcctca tgaataccca ggaaccatct
     6901 catcttttcg acacgctctg gatcaggatc gataggctta taggattctt cgatctttat
     6961 ccgagcgtct gtaaactgaa tcaaatttct aacttcttct ggattgactg cgagctgagt
     7021 tgaatggcca tcgtcagaat actgcgcccg cagccgctta ttgtggattg acgacgcaag
     7081 ttctttagat tctacaaagt ctttacccgt aataactttg tttccaagca tcggcgtcca
     7141 aatcgcccag aacaacttct tttggtgatc gtcttcgtgt ttagctaacc agcttggatt
     7201 tggtttacca gagtattttt caagaagttt ttgcttttta agaagcgact ttccgacttc
     7261 ttctagtgcc gttgtcgcta aaacgtacgc tacattaggg gcgatctgtt cggactcctt
     7321 cgcacatgtt aaaaatctct tagcattttc tactgcgtca ttctgctttt cgtcagctga
     7381 agataacaag cttcgaggtc ccgatctgag tttgtattca tgaagcccgc ggaatccaaa
     7441 atagaccgcg agtgtaacaa aatgaaatat gactaagaac gtctggttgc caaaaaagct
     7501 cttaaaacat aaatatgcga agagctgaca gacggcgacc aacacgaata gtatttcgcc
     7561 aaaccttttc cctctccaaa ggccgaccgc agcggccaag tcagccgcag ccaaaaaaag
     7621 gatccaagtt ttccaaatag agtccatctc ggaaaacctg agacgagcat caaagagatc
     7681 agccgcatgg aaaatgaatc ctgcgaaata aagtgcagct aatactttga gatatattcg
     7741 cactcgtatt cctcaatcca tttttttgag ccactcgtgc ccgccgttat agatttttat
     7801 caacgcatct tttagccaat ttttttgatc atcagacaat accgtaattg cattttcaaa
     7861 atcctgctga tctttagatc gaagagattt tgatttataa agaagctgaa tttctggagc
     7921 caacagcgag gtgccatcgt tcgttgtgac cgttatggaa cttaatctgc gccgaataga
     7981 ctcatctctt ttaaaaatcc attcttcatt ttcggtatcg aatagcatga tctgaatttg
     8041 ccatggatat ttgggagttc ttcgccccca aatatcttga aggcctttcc ccaagatctc
     8101 acctttgggc caaggcttta aagtacctgg cggatcagct gcccacaggt cccaacctgc
     8161 caataggttt tgaatttcaa gttgatcctc acgaaggata agaacatcaa tgtcgtcatg
     8221 cttacgggtt tgcttaccga gaaacagatc aatcgcccag ccacccgcaa tccaccactg
     8281 gtgcttgaag ggcttaaata catttttgac ttcatcgacc gataacggat cccaattgta
     8341 gtcgccaata ttcaatgaat cccaatcata agcgagctcc ttaaaattct ttccgccaat
     8401 ctcatcttct tttgtttggc tcgaaaatgt tgctcctgat ctttcgtaga atttgatagt
     8461 tggattattt tctaagaccc aacaataggc tctcttatag cctaaatctc tcattttgtt
     8521 gaaaccagca gaaagaaggg aaaagccaat tccgttccct ttttcggatg ccaacagata
     8581 aatcgctcct aactcaatga aaccttcgcg atttggatcc ctcggtggtc caaataaaac
     8641 gaatccgaca atcccattct gaccttctgc cacccatgcc cacctttttg gattggcaag
     8701 aatgcttgtc cacatcttaa ccctactctc gaattcgccg gggagtgcat caaggtagtc
     8761 ttggggaacc aggccattat aagcttcctg ccaagatttt atatgaactt
//
LOCUS       DATBTE010000302         3808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1406778, whole genome shotgun sequence.
ACCESSION   DATBTE010000302 DATBTE010000000
VERSION     DATBTE010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406778"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VIG33_11155"
     CDS             complement(<1..603)
                     /locus_tag="VIG33_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271436.1"
                     /translation="MKIAITLFVVSLGSSFVYAEDLTDLANSHTYSFGGACSSQGNWT
                     QDALAGTQSLLVVVEQLKNDPRCGNFSKAMDQHLANLKAEVGTINADSASANKLSSLK
                     EETLALRNFLSGNTALKKNVIQLILNNTVESAITSTDLYQNSIGNTAKSLLSVGSRIN
                     RASTSSLDILNSVMDSALQEQQCLSSPDILGSLMSGMIQVA"
     gene            complement(787..1059)
                     /locus_tag="VIG33_11160"
     CDS             complement(787..1059)
                     /locus_tag="VIG33_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY8271437.1"
                     /translation="MNKAQLIEKIATETKVSKAQAETILDCAIETIKKTVKKGDDVKL
                     VGFGTFTKAKRKARTGRNPQTGKAIKIPASWAPKFRAGAEFKSMVK"
     gene            complement(1162..1398)
                     /locus_tag="VIG33_11165"
     CDS             complement(1162..1398)
                     /locus_tag="VIG33_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEY8271438.1"
                     /translation="MDMKEKKAQHKVKCPQCGLSTIYSPENPFRPFCSERCKTFDLAH
                     WANGTYSIPLEQSAPSRESELTSDLTEEDLDNFQ"
     gene            complement(1404..2159)
                     /locus_tag="VIG33_11170"
     CDS             complement(1404..2159)
                     /locus_tag="VIG33_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271439.1"
                     /translation="MKKHIFLFSGFIISVFIAACAGSDNNGGGGNPGTIISCPPGATF
                     QNGYCVNGTSGITINTGSVGFYSENWHDRTLTPAGNGLSEFLKNAMGVCDRNHSNGGL
                     AGCSAWAAGAFDIVMQAPNTQSNSVQVTFRAKPQMGQFANYAYSLPSAGQLAGALLGF
                     PVVGNPGAVRNPLQLNMTVSVTNNYQGFEARGYGDFYTTANRSLIQVQIAKGKLEDPR
                     FEYRIAYRGQIIVTGTFIRCNTADCGLAMPIGY"
     gene            complement(2277..3707)
                     /locus_tag="VIG33_11175"
     CDS             complement(2277..3707)
                     /locus_tag="VIG33_11175"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEY8271440.1"
                     /translation="MKSLFVLMISVLIFIAATGKRRESIQELGRDAYIWGYPAVSLAH
                     VRESMLEKTQEPKNSINHFFHSFKTPDPLLGNFITVAPENLYGWAWIDLSKEPLVLTH
                     PQIKDRFYSIQFIDAYSNLFSIISNRTHGEKAGTFLITPPGWQAPVPEKMIQIRASTP
                     EILVLAQTFVKDSKELANIAKLEKQHQLISLSRWNDGIETDSFKSDYPAARLKINKNL
                     ALRGLDFYQKLIQIIEKNPPPTKVEIKEVERFNAIGIPHKKDFDKFSFNTEAKTMLER
                     GIFEGEREIQGRLASGLNPKINGWSYELKSSPFTEDYLLRAAISQLYLFSPPAEESMQ
                     MSLDSDSEGRQLNSLYRYILHFEKNDFPAARKMWSVQVLETKKIKSNNSFRIISAIND
                     KSDKLRYNEDGSMDLYFQSEKPKPAQRSNWLPLPRDANFYVILTLFNPHNSALNHKYI
                     APSLIRQEENTPPKQRFTRTMMAVSR"
BASE COUNT         1060 a          750 c          849 g         1149 t
ORIGIN      
        1 agccacttgg atcataccac tcataagaga tcccaggatg tcaggagaag aaaggcattg
       61 ctgttcttga agagcggaat ccatcactga gtttaaaata tccagactcg aggtcgaagc
      121 gcggttgatt ctactgccaa cgcttaataa tgattttgcg gtattgccta tgctattttg
      181 gtaaaggtcc gtggaggtga tggcagattc cacggtgttg ttgaggatta attgaatgac
      241 gttctttttt aaagcagtgt tgccgcttaa aaagtttcta agagccagag tttcttcttt
      301 taaggagctt aatttatttg cagaggcgga atccgcattg atcgtaccca cttcggcttt
      361 gagattagca aggtgctggt ccattgcttt tgaaaagtta ccgcaacgag ggtcattttt
      421 aagttgttca accactacga gaagactttg tgttccagcc aaggcatctt gggtccaatt
      481 gccttgagag gagcaggcgc ctccaaagga gtaagtgtgg ctattcgcta aatctgtgag
      541 gtcttcagca taaacaaagg aactccccag tgacactacg aacagagtaa ttgctatctt
      601 cattaaccct ctccacttct tattactgga cgttactgaa tgatagctat accaataatt
      661 ttatggatat gtgggatgaa aaaaaatatt tgaacttatt actatggatg ttagaggttc
      721 tacagagggc gataagaaaa aagggccgcg aaacagccct ttgatgagtt aaatgtaatt
      781 attatattac ttaaccatgg atttaaattc agcgccagcg cggaatttag gagcccaaga
      841 agctgggatt ttgatggctt taccagtttg tgggttacgg ccggtgcgag ctttacgttt
      901 agctttagtg aaagtaccaa agccaacaag ctttacatcg tcaccttttt taacagtttt
      961 tttgatggtt tcgatcgcgc aatcaagaat agtttcggct tgagctttag aaactttagt
     1021 ttcagtagcg atcttttcaa tcaactgagc tttattcatt ttgactcctc attgtgtcaa
     1081 tgttgtttaa tgtcttttaa tgtctaatga gcacaatgaa aagacaaaaa caggatttcg
     1141 tcaacatcaa aaagttaagc actattgaaa attatcaaga tcttcttcag taagatcgga
     1201 agtaagttcg gactctctag atggggcgct ctgttcgaga ggaatgctat aagttccatt
     1261 tgcccaatga gcaagatcaa atgttttgca tctttctgaa caaaaggggc ggaagggatt
     1321 ttcaggcgaa taaatagtag aaagtccgca ctgagggcat ttaactttat gttgtgcttt
     1381 cttttccttc atatccatga aggctagtat ccaataggca tcgctaatcc gcaatctgca
     1441 gtgttacaac gaataaaagt tcctgtaaca ataatttgac cgcggtatgc aatgcggtac
     1501 tcaaacctag gatcttcaag ttttccttta gcaatttgaa cttggatcaa gcttctattt
     1561 gcagttgtat aaaagtcgcc atagccacgc gcttcaaacc cttgatagtt attggtcaca
     1621 gagacggtca tgttgagttg caatggattt cgaacagcgc ccgggtttcc taccacaggg
     1681 aatcctaata gagctccagc aagttgccca gcagaaggta aagagtaggc atagtttgcg
     1741 aattgcccca tttgaggttt tgcccgaaaa gtcacttgaa ccgaatttga ttgtgtattt
     1801 ggtgcttgca tcacaatatc aaaggctccg gcggcccagg cactacagcc agcaagtccg
     1861 ccatttgaat ggttgcgatc acagacgccc atggcatttt ttaaaaattc actaagacca
     1921 tttccagctg gagttaaggt gcggtcatgc cagttttcag aataaaaccc cacagacccc
     1981 gtattgatag taataccgct tgttccattt acgcagtaac cattttggaa agtagctccc
     2041 ggagggcaac taataatggt tcctggattc cctccgcccc cattgttatc ggaaccggca
     2101 caggcggcaa tgaatacaga gattataaaa ccactaaata agaatatatg cttcttcata
     2161 aagtcctctt attcaactaa caagcagagg ccatgccata aagaggtcta gctctagacg
     2221 aattgggaaa atttacctgt caaagtcttt caaagagggc ccttttttgg catgtctcat
     2281 cttgagacag ccatcatagt gcgggtaaag cgttgcttcg gaggggtatt ttcctcctgt
     2341 cggatcagag aaggagcaat atacttgtga ttgagcgcgg agttatgagg attaaaaagc
     2401 gtgaggatca cgtaaaagtt tgcatctctt ggaagcggca accagtttga ccgctgcgct
     2461 ggtttgggtt tctcactttg aaagtagaga tccatggagc catcctcgtt gtatctcagt
     2521 ttgtcacttt tatcgttgat agctgaaatt atgcgaaaag aattatttga ttttatcttc
     2581 tttgtttcta gcacttgaac cgaccacatt tttcgagcgg ccggaaagtc atttttttcg
     2641 aaatgaagaa tatagcgata aaggctgttg agctgtcgtc cttcactatc gctgtccaag
     2701 gacatttgca tactttcctc ggcgggtgga gagaataaat aaagttgtga aatcgctgct
     2761 cgtaacaaat agtcctcagt aaagggggaa gactttaact cataactcca accattgatc
     2821 ttaggattta agcccgaggc caggcgccct tgaatttctc tttctccctc aaagattcca
     2881 cgttccagca ttgtttttgc ctcggtattg aaactgaact tgtcaaaatc cttcttatga
     2941 gggatcccaa tggcattaaa tcgttccact tcttttattt cgactttagt gggaggagga
     3001 tttttttcga taatttggat aagtttctgg tagaaatcca accctcttaa ggcgagattc
     3061 ttatttattt tcaatcgggc cgctggataa tcgcttttga aagagtcagt ctcaattcca
     3121 tcgttccaac gagaaagaga aatcagctga tgttgttttt caagtttggc aatgtttgct
     3181 agttccttag agtcttttac gaaagtttgg gcaagaacta aaatctctgg agttgaggcg
     3241 cggatctgaa tcattttttc cggcacgggc gcttgccatc caggcggtgt aattaaaaaa
     3301 gttcctgcct tttcgccgtg ggtacgattg ctaatgatgg agaaaagatt cgagtacgca
     3361 tcaataaact gtatggagta aaaccgatct tttatctgtg ggtgggtaag gacgagaggc
     3421 tctttgctaa gatctatcca agcccagccg taaagatttt ccggggccac tgtaatgaag
     3481 tttcccagaa gagggtccgg tgttttaaag gagtgaaaaa aatgatttat cgagtttttt
     3541 ggttcttggg ttttctcgag catagattcg cgaacgtgag ccaaggagac tgcaggataa
     3601 ccccagatat aggcgtcgcg gccaagttct tgtatgcttt ctctcctttt accagtggcc
     3661 gcaataaaaa tgaggacact tatcataaga acaaaaagtg atttcattct ttaatcttat
     3721 ttagcccttg agaaaaactt ctccgaatct taaggagttg tagtaaaatc ttataatcta
     3781 tttatttaca aagttctggc gtgttggt
//
LOCUS       DATBTE010000303         1658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1406780, whole genome shotgun sequence.
ACCESSION   DATBTE010000303 DATBTE010000000
VERSION     DATBTE010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1406780"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VIG33_11180"
     CDS             <1..702
                     /locus_tag="VIG33_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271441.1"
                     /translation="ELIERFEERWYWSRLTENASLPWSTELIERVEECEERRDWFWLS
                     NNEGLPWSLELIERFEERWDWDTLSRNKGLPWSLELIERFEERWKFAGESDHHGLSRN
                     ESLPWSLELIERFERQWDWDRLSKNESLPWSLELIERFEERWNWRWLSGNKGLPWSLE
                     LIEHFKEWLDWGGFSWNEGLPWSLELIERFEARWNWKYLFARNKDKDLPSLRPEDIVA
                     IMEFIEARTKLLKRN"
     gene            706..1218
                     /locus_tag="VIG33_11185"
     CDS             706..1218
                     /locus_tag="VIG33_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271442.1"
                     /translation="MDAYQKQFELRQETPAYWHNKSLDLFISAHTLWVAMQDNKSLEV
                     NCWPTYKMLMGMSFELLFKAHCVGAGINFAADHDLVELARTAKFTLTKDENGILKVLS
                     EYIVWDGKYPIPKKPQRLENHWKNQSQVLSDKKKFGGLTVQVSNDKLDFENLSPFWQK
                     YSDQFMDKYN"
     gene            1426..1620
                     /locus_tag="VIG33_11190"
     CDS             1426..1620
                     /locus_tag="VIG33_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271443.1"
                     /translation="MKNQKQSECSALVVKGGQQYTKKDILHILRQVNKDAFIVEEENL
                     IDVDSVKFFFANGKLSEVAG"
BASE COUNT          475 a          326 c          429 g          428 t
ORIGIN      
        1 gaactgatcg agcgcttcga agagcggtgg tattggagta ggcttaccga gaacgcaagc
       61 ctaccttggt cgaccgagct gatcgagcgc gtcgaagagt gcgaagagcg gagggattgg
      121 ttttggctct ccaataacga aggcttgcct tggtcactcg agctgatcga gcgcttcgaa
      181 gagcggtggg attgggatac tctttccagg aacaaaggct tgccttggtc gctcgagctg
      241 atcgagcgtt tcgaagagcg gtggaagttc gcgggggaat ctgatcacca tgggctttcc
      301 aggaacgaaa gcctgccttg gtcgcttgag ctgatcgagc gcttcgaaag gcagtgggat
      361 tgggataggc tttccaagaa cgaaagcctg ccttggtcgc tcgagctgat tgagcgcttc
      421 gaggagcggt ggaattggcg gtggctttcc gggaacaaag gcctgccttg gtcgctcgag
      481 ctgatcgagc acttcaagga gtggttggat tggggtgggt tctcctggaa tgaaggcttg
      541 ccttggtcgc tcgagttgat agagcgcttc gaagcgcggt ggaattggaa gtatctcttc
      601 gcgcggaaca aagacaaaga cctaccttcg cttcgccctg aggatatcgt cgcgatcatg
      661 gagtttatcg aagctagaac aaaacttcta aagagaaatt aatgtatgga tgcttatcaa
      721 aagcaatttg aactacggca ggagactcct gcgtattggc acaataaatc tctggatcta
      781 tttatttcag cacacacgct atgggtagca atgcaggaca ataaaagcct agaagttaat
      841 tgttggccaa cctataaaat gctcatgggg atgtctttcg agcttctttt taaagctcat
      901 tgtgttggtg ccggcattaa ctttgcagct gatcatgatt tagtagaatt agcaagaact
      961 gctaagttca cattaactaa agatgaaaac ggaatactca aagtattgtc agaatacatt
     1021 gtctgggatg gaaaatatcc tataccaaag aagcctcaac gcctcgaaaa tcattggaaa
     1081 aatcaaagcc aagtactaag tgacaagaaa aaatttggcg gattaactgt tcaggtttca
     1141 aatgataaat tggattttga aaatttaagt ccattttggc aaaaatattc agatcaattt
     1201 atggataagt acaattgaaa tataacaagt cgcttcatcc gactaaaaac cgcgatgctg
     1261 ttttcgccgg gtgagcttgg cgttatgcgt aaccagccaa catcatgaac aagtggaaag
     1321 tttcgttctt tgtagccgta gcagtgctaa tcgcaaccaa cattttctgg ctctattcgg
     1381 ctattgacgc aggcgttaca tacacttacc aacaagtaag ccttgatgaa aaatcaaaag
     1441 cagtcggaat gctcggcgct agtggtcaaa ggtgggcagc aatacacgaa gaaagatatt
     1501 cttcatattt tgcgccaagt gaataaggac gcattcattg ttgaagaaga gaacttaatt
     1561 gatgttgata gcgtcaagtt tttcttcgca aatggaaagc tatcagaggt tgcaggataa
     1621 atacgcataa cctgtattag ttcagactta acctgaca
//
LOCUS       DATBTE010000304         5749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2226491, whole genome shotgun sequence.
ACCESSION   DATBTE010000304 DATBTE010000000
VERSION     DATBTE010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2226491"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="VIG33_11195"
     CDS             complement(<1..1490)
                     /locus_tag="VIG33_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271444.1"
                     /translation="MKLCLHVQLFLTFISIRSFAIGVCSKNPSDLIWVPALKDSLIDE
                     ATKNLPQFCQWNRLDRLIRSFPTEVQKEYHELKQQNNLQGAGDLLSEKAKGMYFDPWI
                     LSMAGHPKVKSLAGQFSYIVSCPEKIGVVCPPANNEDLNLAIRQPFKNPNLQIPKYTK
                     EQSTNSSTLQRAAACTLFSKSSMSECDKNLLKIESFMAIESFQQDQVMIAPYGAGEQS
                     IFSKPGLGFAMGAPMPSSMVTAAPVIKRILTDSKATEGLRKTAIKMIEQQKMKINGKS
                     NLFDDLQNSFVEAGLSKTEAETKTWDTLGALAATGPNFAKRWSRHSSPYMGQKMNLKN
                     IDDNPNAFLLQIIAEAIPALDSKKAAEHQGQPYSLPSGVSFGCDIGKTYHFWMTAYLS
                     RQLTSQGSSPESAASAAYIANLGYQLRREAFAGKELNVKDMEKFGATENGIRMDLVLA
                     ASGAKFGAASANSQVYSIDLSQKLRESMKATPIDATPMSMLGIIPG"
     gene            complement(1753..3417)
                     /locus_tag="VIG33_11200"
     CDS             complement(1753..3417)
                     /locus_tag="VIG33_11200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8271445.1"
                     /translation="MNGQKLIKIVTAALALSTLCRFGELRWTEWKNTQLTQSLCSLVG
                     TPLETGNLRSIYEGIDGGLRHSGLANTCVNVTDNGRSFSPDCIQPEIQYQITMCKAEA
                     NTGVRATVMYPTESMISQTLLLLFLIFTLTFVGFSLLIDRFSKLVMEKFATEIKVRLD
                     LSAHHRTNPSWFPRVVDWFLKESGITEKVNNQADSFRRQLRDYELMITQEASQKFKRQ
                     HEAQASKDYIEKVKSIRHDIRSPLNSLQAVREAMITDPEAERTLFSAIRRIQVMLDQL
                     EEVDRVAEKEHLLIAEVAADDVVFEVKNKFQQAKGVTLNYEFDHNQLSPINAGREGFN
                     NVIDNLMQNAFDAVGKDGVIKLAISSNSSDCIIQVEDNGCGIPLETESKIFGRGATFG
                     KINGIGLGLFHTKKNIESWGGKIYFERLSQGTRFVVKIPLAQTGVQFVGLDPYKKLKV
                     IDDDHTVPKALKNSGFEILEIAETFEKGQELLAAHHGTDTTVLVDYRLNDSKLGTELI
                     DAHPSRQGILLCTSDYDDHALIKKAKALGVKIMPKPLCYSGQLFLS"
     gene            complement(3414..4457)
                     /locus_tag="VIG33_11205"
     CDS             complement(3414..4457)
                     /locus_tag="VIG33_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271446.1"
                     /translation="MSTLTTLQLEPKAISKEIMENQLLEWFAALGLNLREQRMQVGDF
                     EYYHFSSFKKRASGGGYLSSHGRSTSRRTAAIKCAAEMIERQFMLDYFPTAPSFVLAE
                     THNFEKETKTLEIVALPKKDFWSSNGWAVHFDKDISQKKAIDEALERHLLLKSFLNSQ
                     WQGFSPVHQIKNSEMELYFYSSQFELGEKTAGIVLAKSPLYKGVSFGYCLGNKSEMAN
                     ISFWENAIFEAIDKILTLNGKSVDLSQRPDSWIVSDTKEFLEKPFDFSSMSHTPVQEV
                     KDVIPEVSVKTFNLAEKWNLPFSFFASFAWGGSLIPLFNKASLSPTSILQLKAVLKLN
                     GLDKEIPERHPIL"
     gene            complement(4454..>5749)
                     /locus_tag="VIG33_11210"
     CDS             complement(4454..>5749)
                     /locus_tag="VIG33_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8271447.1"
                     /translation="ATFDSSAKKFIPMIAEKWSSQPDGTHIFKLKDGIQFHDGTPITV
                     KDVLWSIKRHLLIKKSTHFRFWEYIKGCGSLKSLEDECEGLYAISDKEIGIRLVEHSE
                     SFFLQMASPETGIFAASDMDPKSFTLKPTKFSGPYFVESIEGKSALLKRNEHSPLSKA
                     FPNSPRTIRLVGISLAEADEAVKNHQLDLVVRSYRPLGESDWKGLGLNTHETSNSSIL
                     YLYGTGLHERKPVGQAFIQKLWELNTDKKINPAGSFLPFSGKGTLVKEEFLAELPQET
                     SKKLRILCPESFFSEDFLKQIKEAGKSVGSEIEFTSIPGMEWIKAFDDPKTAEKFDYI
                     LSSYAASERYPTAQLRILAGKLLQPPIDLKTVEAPNSNLDREKTLRDFQKWLLDTRQV
                     IPLFFNRTLFVHQKNLDLGEQSSTDAEIELWRIQEKVSK"
BASE COUNT         1512 a         1251 c         1274 g         1712 t
ORIGIN      
        1 aagccaggaa taattccaag cattgacatt ggcgtagcat cgattggagt cgccttcatg
       61 gattccctta acttttgact tagatcaata gagtaaacct ggctatttgc agatgctgcg
      121 ccaaattttg ctccagaagc cgccaaaaca agatccatcc tgataccatt ttcagttgcg
      181 ccaaattttt ccatgtcctt cacattcaac tctttaccag caaaagcctc tcgtctcagt
      241 tggtatccca ggtttgcaat atatgcagct gaagcggcag attctggtga agacccctga
      301 gaagtcagct gtcgtgaaag gtaagctgtc atccagaagt ggtaggtctt gccaatatca
      361 catccaaatg aaacacctga aggtaaactg taaggttgtc cttgatgttc tgcagccttc
      421 ttggaatcta acgctggaat ggcctctgca attatctgca ataaaaaggc atttggatta
      481 tcatcgatat tttttagatt catcttttgc cccatatatg gagaggaatg ccgactccag
      541 cgcttcgcaa aattagggcc tgttgcagct aaggcaccga gtgtatccca cgtcttggtc
      601 tctgcttcag ttttcgatag tccagcttca acgaaagaat tttggagatc atcaaatagg
      661 ttcgatttac catttatttt catcttttgc tgctctatca ttttgatggc agtttttcga
      721 agcccttcgg tcgctttaga gtctgtcaaa attctcttaa tcaccggtgc cgctgtcacc
      781 attgatgaag gcataggtgc ccccatggca aatccaagcc caggtttact aaatatggat
      841 tgctcgccag caccgtaagg cgcaatcatc acttgatctt gttgaaagct ctcgattgcc
      901 ataaaggact cgatttttaa aagattttta tcacactcgg acattgaaga ttttgaaaat
      961 aaagtacatg ctgcggctct ttgtagtgtg gaactgtttg ttgattgttc ttttgtgtat
     1021 ttgggtattt gaagattcgg attcttgaaa ggttgtcgaa tggccaggtt aagatcctca
     1081 ttgttcgcag gtgggcacac cactccaatt ttctctgggc aggaaacaat atacgaaaat
     1141 tgacctgcaa gactttttac ttttggatgt ccagccatgg ataatatcca cggatcaaaa
     1201 tacatcccct ttgctttttc tgaaagtaaa tctccagctc cctgtagatt gttttgttgt
     1261 tttaattcat ggtattcctt ttgaacttca gtgggaaatg agcgaattag ccggtcaaga
     1321 cgattccatt gacaaaattg cgggagattt tttgttgcct cgtcgatcag gctatctttt
     1381 agcgccggaa cccaaattag atcggatgga tttttggagc agacgccaat agcaaaactt
     1441 cgaatgctta tgaaagttag aaaaagttga acatgtaggc agagtttcat tctttcgttt
     1501 cggagtttta ccctagatat taaacgattt tgagaatttt aagactttag tctggcgcga
     1561 ttggatttta attatctttt gtcctaccgc cctggtgttc cataagggaa taactctggc
     1621 cgcccggcac cctaactcta atacgcgttt aagctcattc tgtggccgat gtcccctggg
     1681 aacgaatata tcccccggtc gggatctcaa agccacatgg ccaccgcgca ccgatgctcc
     1741 caatcctggc cattaagata aaaacagctg gcccgagtag cataacggct tgggcatgat
     1801 tttgacccca agggctttgg ccttcttaat aagtgcatgg tcatcgtagt cgctggtaca
     1861 caacaaaatc ccctggcggc tgggatgcgc gtcaatgagt tccgtgccga gcttgctgtc
     1921 attcagccgg tagtccacaa gcacggtggt gtcggttccg tggtgggcgg ctagaagctc
     1981 ctggcctttt tcaaaggtct cggcgatttc aagaatctca aagccactgt ttttaagagc
     2041 cttaggaaca gtatggtcgt cgtcgatgac tttgagcttc ttgtatggat caaggccgac
     2101 aaattggacc ccggtttgtg ccagtgggat tttcacgaca aaccgggttc cttgtgaaag
     2161 gcgctcaaaa taaatttttc caccccagga ctctatgttc tttttggtat ggaaaagccc
     2221 aaggccgata ccgttaatct tgccaaaagt tgcaccacga ccaaaaattt tagactcggt
     2281 ttcaagagga atgccacaac cattgtcttc gacttgtata atgcaatcag aagagtttga
     2341 ggaaatcgcg agcttaataa caccgtcttt tccaacagca tcaaatgcat tttgcatgag
     2401 attgtcgatc acgttattaa aaccctcgcg tccggcattg atgggcgaaa gctggttgtg
     2461 gtcaaactca taattcagtg tcacaccttt tgcctgttga aacttgtttt tcacttcaaa
     2521 gacaacgtca tcggcggcca cttccgcaat gaggagatgt tctttttcag caactcggtc
     2581 gacttcttcg agttggtcaa gcatgacctg aattctgcgg atagcagaga aaagggtccg
     2641 ctcagcttct gggtctgtga tcatagcctc tcgcactgct tggagcgagt tgagggggga
     2701 cctaatatca tgccgtatcg acttgacctt ctctatatag tctttagagg cctgcgcctc
     2761 atgctggcgc ttaaattttt gcgacgcctc ttgggtaatc attagttcat agtctcgtag
     2821 ttggcgtcta aaggaatcgg cctggttatt taccttttca gtaataccac tttcttttaa
     2881 gaaccaatca actacgcgtg ggaaccagct tgggtttgtg cggtggtgag cgcttaaatc
     2941 taagcgcacc tttatttccg tggcgaattt ttccataaca agttttgaga atcgatcgat
     3001 aagtaagctg aaacccacaa aggtcagggt aaaaatcaaa aacagaagta ataatgtctg
     3061 cgagatcatg ctttctgtgg ggtacatcac tgtggcacgg accccagtat tcgcctcagc
     3121 tttgcacatg gtaatttgat attgaatctc tggttgaatg cagtctggcg aaaagctgcg
     3181 tccgttgtcg gtgacgttca cgcaggtgtt ggcgaggccg gagtgtctaa ggcctccgtc
     3241 gattccttca tatatagagc gaagattgcc agtttccaga ggagttccga caagagaaca
     3301 aagactttgt gtcaattggg tgtttttcca ctcagtccac cgcaattcac caaaacggca
     3361 aagagtcgaa agagccaggg cggctgtaac tatttttatg agcttttggc cattcataaa
     3421 atcggatgcc tctctggaat ttccttgtcc aggccgttga gtttgagcac agcttttagc
     3481 tgtagtatgc tcgtagggct gaggcttgct ttattaaaaa gaggaataag acttccaccc
     3541 caggcaaacg acgcaaaaaa tgaaaagggc agattccatt tttcggctaa attgaaagtt
     3601 ttaactgaaa cttctgggat gacgtctttt acttcctgaa ctggggtgtg gctcatggaa
     3661 ctaaaatcaa aaggtttctc taaaaattct tttgtgtcac ttactatcca agagtccggc
     3721 ctctgagata ggtcgacact ttttccattt aaagttagta ttttatcaat cgcctcaaaa
     3781 attgcatttt cccaaaaaga gatatttgcc atctctgatt tatttccgag acaatatcca
     3841 aacgacacgc ctttatagag gggggatttg gcgagaacta ttcccgccgt tttttcgccc
     3901 aattcaaact gggagctata aaaataaagt tccatctctg agtttttaat ctgatgaacg
     3961 gggctaaaac cctgccattg actattcaaa aaagacttaa gaagcaaatg acgttccaaa
     4021 gcttcgtcga tagctttctt ttgagaaata tctttatcaa aatgaacggc ccaaccattg
     4081 ctggaccaaa aatctttctt tggaagagcc actatttcaa gagtcttcgt ttctttttca
     4141 aaattgtgcg tttccgccag tacaaatgat ggtgccgtgg ggaagtaatc aagcataaat
     4201 tgacgttcaa tcatctctgc cgcgcacttt atcgcagcag ttcttcttga ggtagagcgt
     4261 ccatgactgc ttaagtatcc gccgccgctg gcccttttct taaaggaaga aaaatgataa
     4321 tattcaaagt caccaacctg cattcgctgc tcacgcaggt tcaaaccaag cgctgcaaac
     4381 cattccagga gttgattctc catgatctct ttagaaatgg cttttggttc gagttgaagg
     4441 gtggtgaggg tactcattta gaaacctttt cttgaattcg ccacaattca atttctgcat
     4501 cggtgcttga ttgctctcca agatcgaggt tcttttgatg cacgaaaaga gttctattga
     4561 aaaaaagagg aatgacctgg cgggtatcaa gaagccactt ttggaaatcc cgaagagttt
     4621 tttcacggtc caggttagag ttcggcgctt caaccgtttt caaatctatt ggtggttgaa
     4681 gtaattttcc tgccagaata cgaagttgag cagttggata tctttccgaa gcggcataag
     4741 aacttaaaat ataatcaaac ttttcggcgg ttttagggtc atcaaaggcc ttgatccatt
     4801 ccataccagg gatcgaagtg aattcaatct ctgatcctac tgattttccc gcttctttga
     4861 tttgctttaa aaagtcttca gagaaaaaac tttctgggca aagaattcga agttttttag
     4921 aagtttcctg aggcaactcg gccaagaact cctctttgac gagagttcct ttgccggaga
     4981 atggcagaaa agaaccggct ggattgattt ttttatccgt attgagttcc catagttttt
     5041 ggatgaaagc ttggcccacg ggttttcttt cgtgaagacc tgtcccgtat aaatataaga
     5101 tggaagaatt tgaggtctcg tgagtattaa gaccgaggcc tttccaatca gactctccca
     5161 gagggcgata ggaacggacc accaaatcca attgatgatt tttcacggct tcgtccgcct
     5221 ctgctaggga tattccgacc aatctgattg ttcttggcga atttggaaag gctttgctta
     5281 ggggcgagtg ctcatttctt tttagaagag ccgactttcc ttcaatgctt tccacgaaat
     5341 agggaccaga gaatttggtc ggttttagcg tgaaactttt cgggtccatg tcggaagcgg
     5401 caaagatccc ggtctctggc gaagccattt gcaaaaagaa tgactccgaa tgctcgacaa
     5461 gtcgaatacc aatttctttg tccgaaatag catagagacc ttcacattca tcttctaaag
     5521 atttaaggga gccacaacct ttgatatatt cccaaaatcg gaaatgggtc gattttttta
     5581 tcaataaatg ccgtttaatg gaccacagaa cgtctttcac tgtgatgggg gtgccatcat
     5641 gaaattgaat tccatccttg agcttaaaaa tatgagtccc atcgggctgg gaactccatt
     5701 tttctgcaat catgggaatg aatttttttg cggagctatc aaaggtcgc
//
LOCUS       DATBTE010000305        43743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_724005, whole genome shotgun sequence.
ACCESSION   DATBTE010000305 DATBTE010000000
VERSION     DATBTE010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 43743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43743
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724005"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..341)
                     /locus_tag="VIG33_11215"
     CDS             complement(126..341)
                     /locus_tag="VIG33_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271448.1"
                     /translation="MTVEKLDYFFPFVVFFYGLLLVGVLENPVLVKIGEERLGAAFQQ
                     VSRHKGLAWICFFVGGLWSIQNVYLTL"
     gene            421..591
                     /gene="ssrS"
                     /locus_tag="VIG33_11220"
     ncRNA           421..591
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VIG33_11220"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            772..2664
                     /locus_tag="VIG33_11225"
     CDS             772..2664
                     /locus_tag="VIG33_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271449.1"
                     /translation="MDLRSGSIQLCWYLIGFSLLALGCQSSIEPDNTAAYAQNPEASY
                     CGSAQARTYSSNTTTISGTATFTYRPLQTASNCGLCGDPVSLGVTYAEVHILDSSGNV
                     IQCGETDQLGTFSLMIPRVAGSYKVQVLSRSLTDKVKASILKDIYSATPYTVETAFSL
                     NSTQSTASGISVVASARASDDSSIPGGAFNILANIFWANEFIRENSSSSNFVADKVSV
                     FWKAGFNPYSYFNAPSVLASFYVNTEDRLYILGGKNDDVKSSDTDHFDDSVILHEYGH
                     FLEAHYGHSDTPGGSHNGNFIIDPRLAWSEGWANFFQGTVLNKIVYSSTYTGPKKGSS
                     GFSYYIDTVGFANDSVEGSGSSYNIQISRDLSELNSMAYYDKAAIDEGIFREFSISRT
                     LFKTITNTDGLPFAAIWDSFKELRNSHSPAYKFVNFGLFNSILQTLLNANYSSLDQST
                     WNTVIGEEYQKKNTDLYGRYLGGTQAPSGGTPACPEIIMDPVMDSIYTYDRSNQLKSN
                     DFFTFYHDGTSKQMGLKARSTGNVIDMNLYLYADGYNYSEELQEAQAGFNNTSLLRKS
                     ATLNTASSTETEVIDFSGLPAGYYMVNVKASTLNKTSANLNGGTANYYLYFGNSTTVT
                     TCLIPN"
     gene            2646..3230
                     /locus_tag="VIG33_11230"
     CDS             2646..3230
                     /locus_tag="VIG33_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271450.1"
                     /translation="MSNTKLILFSKMTLLGVLGLCLGGTGMFYYQKQHRIHRSVASIE
                     PFQPHGLTLGKQAAAMTIEIVGPENYPDNNSEIVELVGYITQHLAANSSLAYEWSLPE
                     GVELMRGPLSDTLGNLQMGRPRQVSILVRGFSRERQKLISLRTQVIAGNAPLVASATI
                     VSRPEDTMESKVMDLQVQAKAAAQAAKESETESR"
     gene            3441..6047
                     /locus_tag="VIG33_11235"
     CDS             3441..6047
                     /locus_tag="VIG33_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271451.1"
                     /translation="MFRFRNVLRLSCALFVAGSMVGCSKSAKDDASSSTSLSSSLISE
                     LSQALGSVSVSSLGQQKMMKSSEFHTLSTPLTDVQIQAIVDSAKTAVESANLNSSDDL
                     IALLPVIAAGTEDKLDQINIPSASDKIAIIEAISESLLTSMSGREANLPTGSADMGLT
                     PAETAVNKLSSAIISHFADAGLEATDIPTASEAAMNTMIVNLGSAGIPLAGLDGAVKQ
                     MTAGAVSGLDNISVSGFSASNLPTVVASLTAGATKGLGSISTSGYDSSKLPDMVKNVA
                     AGATGALGYISMTGYDKNNLGPMVKQISGGIVGALDGISMTGYQASSLPSMVESASAG
                     VVANLGSINISGYSATDVSSLASAATAGATGALDKIVMTGYDADSIATMAAKITTGAT
                     KGMGNISMTGFDVTNLPVVAKSITSGATGELGNIVMSGYDATKLPTMVEKISAGATGA
                     LGEITMTGYSSSNLNDMVKQISAGTTAALGTINMTGYDATSLPKMMEGITSGATGALG
                     SISMTGYSVSNLGDLAKNISAGATASLGDISMTGYSATNITEMIKYASSGATQALGNI
                     TMPGYDNSKIQTMVQSISQGCTDSIGSITGLGTINASTAIQSLAEGVSLGAQGIATVY
                     PTVINTSNLQSWIQTGASTTANGYTIPNLTASVNTGYNADPIASFVNFMDTNTSHGII
                     AGHIVIGASMNETAVTSYRVYFGTNPTTKTGSAITTQTKSSGANLDYTLANTTVPTEA
                     THLLVYVVKTDTTETSVPVAIPLMDLSSAGATVNGAANVMLNNTYTPVGMINGKQAYM
                     ANGYFIFWESGYWILHSSNTLTMPGMETMTSASKLQMVQLYLPSTASTPPSTGWLVGY
                     NGASSTATVSAN"
     gene            complement(6091..6651)
                     /locus_tag="VIG33_11240"
     CDS             complement(6091..6651)
                     /locus_tag="VIG33_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEY8271452.1"
                     /translation="MNAQDVLIRTQATPNPAAWKFVLDRPVLNEGKATYSNIEEAAQN
                     HLATGLFDVPGVKQVHFFQNVITVTHSFDADSDEIQKAVCAVIQARMAVHNPNQTQFD
                     ERKMRRQNQSPEMQHIEEILDRTVRPGLQGDGGDLDVVSYEDNKLTVFYQGACGTCPS
                     ATTGTLMAIEGILRDEFNPSIEVIAL"
     gene            complement(6648..7874)
                     /locus_tag="VIG33_11245"
     CDS             complement(6648..7874)
                     /locus_tag="VIG33_11245"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEY8271453.1"
                     /translation="MSQDSFFEKVRSQFPALTQKVNGKDLVYLDSAATTLKPQSVAER
                     IYNFNQYEISNVHRGAHSLADKATVNFENARSAVAKFLNARSAEEIIFVRGTTEAINL
                     AAQTYARTLLKEGDEILLTEMEHHANIVPWQMIAAEKKVKVQAVAVTDKGELDLEDFM
                     VKLSAKTKIFAFTASSNVLGTINDIRFLTEAAHKVGAKVFVDGAQAVAQEVVDLQALD
                     CDFFAFSGHKLFAPFGIGILYGKKELLEQMPPYQGGGSMISKVSFEQTTYNEIPFRFE
                     AGTPNVEGAVALHAAMDFIKSIGFDSIQAHKKELLSAATEGLLKIPQVKIVGTAAHKG
                     PVLSFNLEGAHHSDVGQILDQQGIAVRAGHHCCQPLMKRFGISGTVRASFSVYNNMQD
                     VESFLKAVKKAQELFQ"
     gene            complement(7876..9150)
                     /gene="sufD"
                     /locus_tag="VIG33_11250"
     CDS             complement(7876..9150)
                     /gene="sufD"
                     /locus_tag="VIG33_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEY8271454.1"
                     /translation="MNLLTHFDKFAKQNPAPEPEVQALREKALEFLNVKGLPTRKDED
                     WKYTSVKVLNDETFVPTEFNELNPSPETMRRIQEKLNPAFINIVFCNGVLNKTLSALE
                     TLPTGVKLKQDKELTARDFKDSFEALSTVYFTKNYKLEISAGTAVDKVVNMVFYSSLE
                     GGPAVMINPRFALKLGKHSSLKFVESYFGQKEARYFVNSQVQIEVGEAAKLTAIRVQE
                     DSQRAIHMGRTTFHLDKAAHLHSLVFSTGALLSRHNLEMELKAVEVFATVDGVYLAKG
                     HQHVDNTTLLDHQVGACSTTQHYKGILAGASRGVFNGKVIIRPGALKANSEQLNNNLL
                     LSREAEADSKPQLEVYADDVKAAHGSTVGQLDREELFYLESRGISPQTAIPMMSFGYA
                     SELIYKLENEELQNWLNGELREAFTGLDMAVK"
     gene            complement(9153..9908)
                     /gene="sufC"
                     /locus_tag="VIG33_11255"
     CDS             complement(9153..9908)
                     /gene="sufC"
                     /locus_tag="VIG33_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEY8271455.1"
                     /translation="MLEIKNLHAKVEEKEILKGLNLTVKAGEVHAIMGPNGSGKSTLS
                     KVLAGHPAYTVTQGEVKYEINFNMVNLLDLAADERAKEGIFLAFQYPVEVPGISNFNF
                     LHTAFNAVLTHQGSEPMNEADFRNYVEEKMKLVLMKKDFLDRPVNVGFSGGEKKRNEI
                     LQMAVLSPRLSLLDETDSGLDIDALRIVSEGVNKLRRKDNAIVLVTHYQRLLHYIKPD
                     FVHVLANGKIIETGDYTLAEKLEERGYDWLVGV"
     gene            complement(9913..11373)
                     /gene="sufB"
                     /locus_tag="VIG33_11260"
     CDS             complement(9913..11373)
                     /gene="sufB"
                     /locus_tag="VIG33_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEY8271456.1"
                     /translation="MDNNPKAVQTLPDTEYKWGFVTDLETDAVAKGLNEDVIRLISAK
                     KNEPEWMLEYRLKAFRYWLTMTEPTWAHVKYPKIDFQNICYYSAPKKASEGEKTGPKS
                     LDELDPELIKTFEKLGIPLTEQKRISGIAVDVVFDSVSVGTTHNQILEDVGVIFCSIS
                     EAVHKHPELVKKYLGTVVPFSDNYYAALNAAVFTDGSFCYIPKGVRCPIDLSTYFRIN
                     AKETGQFERTLLVCDEGGFVNYLEGCTAPQRDENQLHAAVVELIALEEAEIKYSTVQN
                     WYTGDKEGKGGIYNFVTKRGKAAGKHAKISWTQVESGSAITWKYPSVILQGDSSEGAF
                     YSVALTHDYMQADTGTKMIHIGKNTKSTIISKGISTGHSSNAYRGQVKVMPSAENARN
                     YSQCDSMLVGPDCSAHTFPYIEVKNKTATLEHEASTSRISEDQIFYLQSRGLSMEQSI
                     SMLVNGFCKEVFKELPLEFSVEAVKLIEMKLENSVG"
     gene            complement(11366..11863)
                     /locus_tag="VIG33_11265"
     CDS             complement(11366..11863)
                     /locus_tag="VIG33_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY8271457.1"
                     /translation="MNKLNRKLEYALMALKHMSHKIPGELTSAKEVSESFATPFDATA
                     RVMQAMAQKGLLRAEQGAFGGYQITKDLGKVTMLQLLEIIEGPTTMVKCMHKESPCEI
                     QGTCNILSPVQLLQYKLNEFYSNITLKELLIETPSSLGNVKNQKRKVPMMATADVNAE
                     DVFNG"
     gene            12065..12934
                     /locus_tag="VIG33_11270"
     CDS             12065..12934
                     /locus_tag="VIG33_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271458.1"
                     /translation="MKMLQYKLRDRPLITMRVLFISAVSIIVLFIVLLISRLWGMGQQ
                     YSEFHHPFFEGAPSPLIIVKVNTEKALQEAAQQKSDVAFWLDVETTNDQKIIVFAHDI
                     QAKEMSIQTFRGPKPLAYPFQQLHRMRPEVRELKRILAQFPTQKFVLNILDNVTGIHT
                     GLSENLKGLSPEKRILIQSNYNVILTSLKEIEPFWLYGCSKADLMRFLTFESMWVLPA
                     TPFKGDVYIAPFFLVGRPAFHESTLEEVRRRNKKIILGAVLDKKEFDDALRLKADGII
                     VENFGDYLNWSRP"
     gene            12986..13729
                     /gene="tolQ"
                     /locus_tag="VIG33_11275"
     CDS             12986..13729
                     /gene="tolQ"
                     /locus_tag="VIG33_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEY8271459.1"
                     /translation="MNIPIINIANAASNTQVAINTSPMDALTQASPVVQLTLLILVSL
                     SIFCWAIAYSKYKQLQQVRAANEHFTTKFWKVNSLDTLYGDIDEFPFSNMARIFKSAY
                     IEMKKLADSPLVKNSGEDKPQLSGLDNLERAIRKSTENEVAKLETRLTVLATTGSTGP
                     FIGLFGTVWGIMGSFHKIGATGNASLAVVAPGISEALIATAIGLAAAIPAVILYNNFV
                     GLVRREEIELNNFSADFLNIVKRNFFKGD"
     gene            13732..14151
                     /locus_tag="VIG33_11280"
     CDS             13732..14151
                     /locus_tag="VIG33_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY8271460.1"
                     /translation="MGMSNGASSKSRTTLSEINVTPLVDVMLVLLIMFMVTTPLMQQG
                     IEVDLPKTSSAGVELNDEPFVLVIDAAQKMTAGKQNIVMGDLRPKLKAIFQHRKNKQV
                     YIQADRKVDYGFVAEAMGEIRAAGVFNIGLITVPKDK"
     gene            14184..15002
                     /locus_tag="VIG33_11285"
     CDS             14184..15002
                     /locus_tag="VIG33_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY8271461.1"
                     /translation="MSSKQKSLIQDKDLAAGLKISVGLHVALVAAFSIRAAFFEPEQI
                     DYTAAVRVDIVALPDKRPDHVPPQAPAKQEEAAKAEPKPKTEPTPVGPAQTSKPPPQE
                     VKSKVPKDSEAINLEGKKAQKEALEKLKAMAAIEELKKSMEKEASKKIAGMGEGKMQS
                     NTVKGNILSPGTSLTGLNRLQHDSYLAGLDQQIKQNWFLPQWLSKKPLRTQVRLKIDE
                     KGHILSREITLSSGNSSYDELALETIDKSAPFAPPPEKFVSIVAVNGLLIGFPE"
     gene            15022..16440
                     /locus_tag="VIG33_11290"
     CDS             15022..16440
                     /locus_tag="VIG33_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation protein TolB"
                     /protein_id="HEY8271462.1"
                     /translation="MLKFLIASMITVTALSFPSAFAQQEAVREPSAATSSAASDIKLG
                     EAHTKKSLLAFPPLNYQGSPSGSYQATGAELYNTINNDLNVSAFFQMIPQKAYLEDTS
                     KTGLRPAPGEPNGFKFQSWSTVGAEFLIRTGFFIAGGEVNLEAYLYHVGKSQLIMGKK
                     YKGPISGVRKIAHTFANDVVEALTGKTGMFLSKIVVSSDKNVGRFKEIYIMDWDGANP
                     EKVSNHRSISLSPAWSPDGKKIAYTSYVVRTKTKMRNADMFLYDLTTGKRNLISFRQG
                     LNSGACFAPDNKTIYLTISQQGTPDLYKMSFDGTLEGKITNGPNNAMNVEPAISADGT
                     KLAFSSDRSGRPMIYTTNPNGSDAKRITFAGVYNSTPSWSPDGKKIAFAGQSEDHFDI
                     FVMNTDGTNMIRLTSAKKPNGRWSTNEDPSFSPDGRFVMYTSNRTGKSQIFISTVDGS
                     EERRVTLDNANYFKPKWSNNIE"
     gene            16433..18862
                     /locus_tag="VIG33_11295"
     CDS             16433..18862
                     /locus_tag="VIG33_11295"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-synthetase adenylyltransferase"
                     /protein_id="HEY8271463.1"
                     /translation="MSNLKCPLKPLSSYTASEISEAIKFSPALSHYLGFTFLHSQTPY
                     HEVRTNAWILSALATILNSENPGKICKTWSHVANDILQSVFRECFKDSAIALFALGKL
                     GAQELNLSSDVDILVVSKEENVHHLKALRQFQKVLSERTPRGYLFRVDFDLRPGGRQG
                     PLIPTLDQFEDYYGNYGETWERLAFVRLTPIAGDSNVISQTLSFAKKFSYRKHLDYTL
                     LEDLKSLRRKIRQHYAPNSTESVWDLKLGVGGIRDVELFVHAMQVIHGGKNPKLQVHP
                     TADALALLEETEILPKVDAQFLKNHYWQLRQLENFIQAQNDEQTHLLEKTTPMPEWAE
                     SILTHLNKDFEHCDHIVSSLLGKASATSSAAFNVVPLQNTKSWEVWREILQIEVLSRN
                     KDRDEQARLQFLNEFYETLQTQKGDTDKALQHLKEFIKSTRAKASFFTLLIRNKNLMD
                     ELAWLFGHSPYLSQILSSRPELIDSYVYRSQEFQKGDLAVLLEQLVEKRLLGEIINGS
                     HYLEDKNLEELQANLSATADEIAGTLLAELKKEYPSELEILCLGKWGGKELGFRSDLD
                     FIFITPHEPTENDSKVARRFINRLTETRKGGSIYAIDTRLKPSGKAGPFVISESQLLT
                     YLHDEAQAWERQAYLKSRKLNSGISSTPQQILNSFANKKLTETELQELERIREGLIRY
                     ESTSFDLKYAEGGMLDIELFSQAEILAESLAIRGPSTLDFLSVIANSDGLQRNYLRLR
                     QIEQMLQLVSTEGGAKVVLNHESFQQLAKALQLSAEDLKTEFNSLISENLQILNQLDR
                     RRRSKILKAAT"
     gene            19092..19598
                     /locus_tag="VIG33_11300"
     CDS             19092..19598
                     /locus_tag="VIG33_11300"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8271464.1"
                     /translation="MIAVFETNKGTFKVKLFNEKAPKTVETFVGLADGTREWTDPKSG
                     NKVKKPFYDGLKFHRVIPNFMIQGGDPLGTGTGGPGFRFEDEFPAGAPKHDKPGILSM
                     ANAGPNTNGSQFFVTVAATPWLDGKHTVFGEVIEGMDVVTNISKVKTGAMDRPAEDVV
                     INHLKIQK"
     gene            complement(19775..20584)
                     /locus_tag="VIG33_11305"
     CDS             complement(19775..20584)
                     /locus_tag="VIG33_11305"
                     /inference="COORDINATES: protein motif:HMM:NF022426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HEY8271465.1"
                     /translation="MSHRFNKILSLVLVLFTVGCANWALQDKCEKTNWFEYSQNVAYQ
                     GKYLEEDGLIKACKGIDSTSAVQLDLGFKQGREKMCQYDEIFTRAKEGIPVFFKFCDG
                     LDMNRMRSLYQQGLVTYCTAEKGYSFAKAGKLYQNLCSPQQEKAFLPGYYKGRKEYLT
                     NYINELNSKLVAVKELEASYARTETNIHREYATLPSNATECHTITVYNDATKQNEIRS
                     ICEEAAYIRGRRSVLWSDLDHIRIKLREVRADWKDIESNLSQAQTDLNSIP"
     gene            20788..23724
                     /locus_tag="VIG33_11310"
     CDS             20788..23724
                     /locus_tag="VIG33_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271466.1"
                     /translation="MKWSKIITSYLVIAQLVLAPVLSHALNGQLDIDNNGRLTEEGKS
                     TVAAAKNYAKENKLKGRLDLANKKITFNDPKTNKVVGEMALSDEKALLEYSPKTLNQK
                     LMKELVKVKAANSAAWSHATRSFAGESAMFFLVLGGMVGIKLMHDYASDPVAMKHHIE
                     QQLSPVGQMAFFMFMYSQGVTANTLSLWIKNPKLGLPIAMLGMTVGSAVQTYFSQVAA
                     DPHIRNCAASVFKGEKIEGNDHPCEGAYKYLVIDKKILEGPGVIALLGAFVASTGIRV
                     ALGASMKLVGFEIGMWLTPGGMAMKFARVLFSAANITSFTLFQAKIEHFATGLWKNYY
                     DGWEFVEINDRLVSHIENQKASQWKSEAKDFNKEVREFSGKMKEWRITNLSDAYTANQ
                     SWGEFLNNLSSMYSASYSFYSTYLDHLKGKNSFIDRPYPLFGVIPKGLVAGHEDLYLT
                     HPHRIEFMQIETINDVAAWIEQTTQSGYYKKLGFFKNQEDIISKIQQGLASEDISTKG
                     TALLELNKVIAKDAKTVTGSLFGRELRRIHSLLGKPYPLLEKGRGFASSMLYAPSMIS
                     AFGDAKADDSNGHFSTPNITDYLVVQMMCGPNPSKGEKVVSVTQGFPAKFMAPTIALD
                     DPSKKDLCTGTFLSRVRSERIFKDMPFSNYPTAPDFLRANLNPVVAEDFISWWEKGTE
                     TQMKEAYKGFRVSFKEVLSKLFKGLNQIQNNPWNRGFISNGAIPSAFQEMRLYALILG
                     ELLKDSYKVQYERDLPTSYYSDKIDPQLRATTSDYLTMSKKPLLGLLGRGSVYSFNAL
                     VGAEPNTKEVHSLKIQKELELLFSQMDGLVQRAKAHVVKSEEFENQMKAIEKKLTEFG
                     ALLGVSKDGVNIFAKPDSEAKNSDSGEETLAPAPRPEPSTLLKITKDSSASSPFLKPE
                     VSVPSHDTLSGSDKNGPNQGLVTLDKEQKALAVTCLELLQSVAQELSMYGHMAGTALF
                     EEKKQD"
     gene            23811..24512
                     /locus_tag="VIG33_11315"
     CDS             23811..24512
                     /locus_tag="VIG33_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271467.1"
                     /translation="MKKKFIVGMMIVTSTLSFSAHAAGTAVEARPAREVLKTYVEDVK
                     KWAAKKIGENRASLPVGEKMKTEKAAEIFMEELKVPAADKTSIKSAMGKSKNKKAFND
                     SLATIMATRRMTEGKSDVESKSLEEASDALIGVLADAKDIGSTANSKFFNEKTTDFAD
                     TTAALNKVVEMPDKFILFDTKTRDQFTKALVKTRELSYKSETYEEAFVKALMESQGID
                     RAKAMEIVRKLKDCV"
     gene            complement(24584..25357)
                     /locus_tag="VIG33_11320"
     CDS             complement(24584..25357)
                     /locus_tag="VIG33_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8271468.1"
                     /translation="MRKRHEEHENHERWLVSYADFITLLFAFFVVMYATSTNNLGKQK
                     EFENSVRANLRLGIGAAAEEKPTSAAPDASKIIDEYIQSKDGQENGGAQSFPQKGSNA
                     EVADYATRYLGKQMNAVTQKELGLEVHHDALGVRVSLAASSVFPEGEFKMKRSSLKIL
                     GQIGELLRQTDKKILVEGHTDDQPISSESVASNWELASLRATSVVRYLIKSQSLDPRR
                     MAAISYADQKPLVPNDTPENRAKNRRIEILIVMGDEKVN"
     gene            complement(25363..26112)
                     /locus_tag="VIG33_11325"
     CDS             complement(25363..26112)
                     /locus_tag="VIG33_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HEY8271469.1"
                     /translation="MDKATWFGLLIGIGGILLANTLDGGQFGSLLQGTAAMIVIAGTL
                     GAVMVSNNTKDIKIGFELASQAFQEKESDIDYKINEMVDYARIVRKENVMALEEKVSK
                     ASDPFIKEMLRTVIDGIEPATIKEIFENKLDSEEDELLSAAKIWSDAGGFSPTIGILG
                     AVLGLIHVMGNLSDTSKLGAGIAVAFVATIYGVGFANLVFIPLGNKIKKKIAQRMKDK
                     RVLLEGGLLITSSLSTMIIDRKLRALAHELK"
     gene            26204..26773
                     /locus_tag="VIG33_11330"
     CDS             26204..26773
                     /locus_tag="VIG33_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEY8271470.1"
                     /translation="MSFLYELDKSLLLKINQEWTSSGADIFFPAITDLHKNPWFFGAI
                     ATTLLILFVWKYRKVGLVFFVVLLLALGSTDLIGNHIFKKNVARARPGDEPSAMVIVR
                     SPYGGYSFTSNHAANMFCLAKYTSEFIPQVRLITYSAALLISYSRIYNGVHYPSDVLG
                     GGIMGWIVGWIYSILGKNIYNRLRKRVVS"
     gene            26770..27762
                     /locus_tag="VIG33_11335"
     CDS             26770..27762
                     /locus_tag="VIG33_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY8271471.1"
                     /translation="MKVLVTGANGFLGSWLTQALVKQGHEVFALVRAQSNLSELKDVA
                     CNYVYGDITDLESLYKSFAGIDTVFHLAGLIAYKRSDRAKMEKINVFGTENVIDACLT
                     KKVRKLVFVSSVVAVGAGFTPEEVLHEESPYNIEHLNLGYFETKRAAEKLVINAYKNK
                     HLDCVIVNPSTIYGKGDAKKGSRTTQVKVAQGRFNFYTSGGVSIVAVEDVVAAIITAW
                     NKGRSGERYILSGENITIKDLFTIIAEEAGVRPPQHKMPTWMIFALGHLGDFLESLGI
                     KGALSTENARTATLYHWFDNSKAKKELNFNPHTAREAIRNSVSWMKENGLLSSK"
     gene            27759..28598
                     /gene="mtgA"
                     /locus_tag="VIG33_11340"
     CDS             27759..28598
                     /gene="mtgA"
                     /locus_tag="VIG33_11340"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEY8271472.1"
                     /translation="MKKFFKRFAFTCLIILTGVSSFAAYVFYKIPSEKEIKGCLVTTM
                     YQVNLCPGSKDYVPLKSISTYLQKTVVLTEDSAFFQHDGFDWKSIEENAKKNLEKGHY
                     AKGGSTISQQLAKNMFLYKDKTLLRKGLEALITTKIEKTLTKKEILERYLNVVEFGKS
                     IYGVKQAANFYFKKTPAQLDVVESAFLAMILPNPIKYSQSYFRKELTPFAQKRLKRIV
                     TDMYQYNRITEDEYNVAIAEVSSFFSPNPMPEDSNVIESSDPTLEELENLGNDEGKDN
                     SFF"
     gene            complement(28618..29406)
                     /locus_tag="VIG33_11345"
     CDS             complement(28618..29406)
                     /locus_tag="VIG33_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8271473.1"
                     /translation="MKFVEIIRPPKINSGFIRVWSRNFLYFRKTWTVSLFWVVLEPLM
                     YLGAIGFGLGAFVSNINGHSYVDFFFPGLLCSTGMMVAFFEGTYGGFTKLTHQRTYST
                     IMLTRIGPEEIAFGELLWSCTKGYFSVCGVALVAAFFGLIDTWRIFPALAVLLILCWL
                     FSSIAMIMTSIARNYDSFIYSTSGFIVPMSLLSGTYFPIEQLPGGLKYLAYLFPLTHA
                     VQAVRGLLQEGFTTAVSIHVLVLLVLGLLLMNIAIYRIRHKLLK"
     gene            complement(29406..30332)
                     /locus_tag="VIG33_11350"
     CDS             complement(29406..30332)
                     /locus_tag="VIG33_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8271474.1"
                     /translation="MSQKIAVELKDLKKKYDENFVVNGINLEIPKGECFGLLGPNGAG
                     KSTTMKMMYCSALVTSGELYVLGLNVKKNYREIKTRIGVIPQEDGLDPDFSVLENLLV
                     YASYQEIPARAASGKAQLLLREMRLEEYADRSVDTLSGGMKRRLAIARGLLNSPELLF
                     MDEPTTGLDPQARLWIWDFIKHLKVENSTVVLTTHYMEEAERLCDRVAIMDHGRILAM
                     GKPADLILEHIGIEVVEFETNPVDLNYYIGRLREGGYNYQVIQDTVMILINPNQEGRK
                     AIDLIASDKILIRKPTLNDVFLKLAGHALRDE"
     gene            complement(30332..32146)
                     /gene="typA"
                     /locus_tag="VIG33_11355"
     CDS             complement(30332..32146)
                     /gene="typA"
                     /locus_tag="VIG33_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY8271475.1"
                     /translation="MIQDPKKIRNIAIIAHVDHGKTTLVDHLIKQAGTFRENEHVEER
                     LMDSMDLEKERGITIAAKNASFTYKDIKVNIVDTPGHSDFGGEVERILNMVDGCILLC
                     DASEGPLPQTRFVLKKALEQNLKVIVCINKIDRSDARIQEVHNELFDLFIDLEANEEQ
                     CDFHTVYAIAREGMATLDPAVNTGSLEVLYDAIINLVPPPKIVENAPLQVMVSNISYN
                     DYVGRLAIGRMRSGTIKVGDEVLCVMEHAQKKVKVSALFQYKVNSQVPAQEVGAGDIV
                     VIAGMEDFTIGDTITSVTEPIPLPRIRVDEPTVGMIFSVNNGPFAGMEGKNVTSRKII
                     ERLERELLYNVAIRVEKTDLTDSFKVIGRGELQLGVLIEQMRRENFELLVSKPTVIFK
                     EENGQKTEPMEIAVIDIEDPYVGVVTEKLGKRKGIMINMVQKGSGRTRLEFRIPSRGL
                     IGYRSTFLTDTRGTGLLNTQFDGYEAFKGEIEHRMNGAMISDRKGMAVAYAIWNLQER
                     GVMYVNHGDEVFEGMIVGEHAKENDLVVNITREKKLTNVRASGTDEAIRIVPVRPMTL
                     ERAMEWIKDSELIEVTPKTIRIRCRELDSSRRGKAARE"
     gene            complement(32170..33246)
                     /gene="dusB"
                     /locus_tag="VIG33_11360"
     CDS             complement(32170..33246)
                     /gene="dusB"
                     /locus_tag="VIG33_11360"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEY8271476.1"
                     /translation="MIPLAPEAVFCYTLTFRMHPLEALKQNPFVLAPMAGITDHAFRT
                     FMKKRGASVVVTELVSATGIEYKSERTLKLMSFDKTQRPIGIQLFGEDPEILARGAQV
                     AEAMGCDFVDLNFGCPVPKVVKKGAGSGMLRDLPLMEKVLGTVVKAVKIPVTIKIRTG
                     WDANTLNAHEVCNIAYNEGITWVAIHGRTRAQGYSGQANWDYLTEIKAKARLPILGNG
                     DILTPQQAVTRMKESGCDGVLIGRGCLKNPHIFMDALSLWRGEELSQQFSRDFMGLFM
                     DLREAIVAHCDEYIVGIQLRKFAAWFSTGYPGASQFRKNLFQTKSNDELMGLAMEFFK
                     SIGTIEQEEREDTSHEEFLMGGHG"
     gene            33375..34142
                     /locus_tag="VIG33_11365"
     CDS             33375..34142
                     /locus_tag="VIG33_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY8271477.1"
                     /translation="MADDIFKKPLETPYLEPALECDFGAFAKVVESRRSVRVYTEEVI
                     PDSVVEKCLDMALLAPNSSNLQSWDFYWVKSPEKMQKLKEYCLNQPAAKTASTLIVAV
                     ARPDDWKRGQKINTDYLAKLKNPPASMKSYYEKLVPMVYGNGPLHLLAPIKSLLFAAI
                     GLFKVIPRGPFGRSGNRLWATKTTALACENLMLAFRAAGYDTCPMEGFDEVRVKKLLQ
                     LTYGASVTMVISAGKRAPNGVYGERLRGPKEMFVKIV"
     gene            complement(34150..34746)
                     /locus_tag="VIG33_11370"
     CDS             complement(34150..34746)
                     /locus_tag="VIG33_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271478.1"
                     /translation="MNLKIRLVYMTFSWLLPVMVFGEVKPFICEQVFQQEVSFTSLRQ
                     KAIQKSSIAPYFKDDYVLLYGSFANGKADIQDSDIDLHPGPKFLQQYLNNRLTSKYNK
                     PEKDPHSEYSTIIPTAESIEMSERLLKTEQILAQLTSRSSYDPSQFMTIVPSNGSFFK
                     IQNLGYYNSLIVKVTHSQLSLYIVDTFGSGQIFEVIIN"
     gene            complement(34989..35588)
                     /locus_tag="VIG33_11375"
     CDS             complement(34989..35588)
                     /locus_tag="VIG33_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEY8271479.1"
                     /translation="MNKEEFYNLLKFRRSIRKYKKDPVPREVLERILAAGMEAPSGKN
                     RQNWRFFVVQGKKRDEYLKYSQKSWLGIKNVLQQRLKPSLYDFTERFFFTLGDAPVLI
                     FAYSHNDNEERYHTSIGSVYMAVENMNLACFAEGLGCCTMGAPLEIKEEVDRFLGVDQ
                     LPEYKKGELELLCGLVIGYPDHQPPKAPRQVEGRVTWLE"
     gene            35626..36123
                     /locus_tag="VIG33_11380"
     CDS             35626..36123
                     /locus_tag="VIG33_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271480.1"
                     /translation="MESKKNTISAENLFSPFLGVLIFLALLVTATAVTPSWRQKVRDF
                     FNPEQRKILAKTSGDLTGEGLQITVLKIKTRDHLVLEVYRAESPDSLNLIARVDLPEK
                     RDAYFQLKGNATNLGLVDVDNDGTLEIVAPSFDEQMIARLNIYKFNPATQSFDRLSAP
                     AETEF"
     gene            complement(36241..37857)
                     /locus_tag="VIG33_11385"
     CDS             complement(36241..37857)
                     /locus_tag="VIG33_11385"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8271481.1"
                     /translation="MKQWFKGIRGRLLMATCLPIVALTVVIGLSIHTDHKFSGLLNES
                     YSRLIPNLNVFIDIREARPRISFYFWAAYGTAASDSVLSKKYIATAETAFDDLKVGIK
                     KYEGLPFGPGEAELFAPTKLLIPKFYTLTEKIIDQLKKGDPESLKAAFENINAGGEWN
                     TLSTQIKEGFDKISQSYIKEVEVSDKEQRELGSFSQNILLFTGAFAILTIFSILMWIA
                     YQVSNSVGQVATQLTDSTGQVTQAIEQLSAAGHALSESSTQSAASLEETVASLEEMTS
                     MVQMNSDHAREAAGLAQSSRESAENGEREIKNLVESMRGISHASKKIEEIIHVIDDIA
                     FQTNLLALNAAVEAARAGEQGKGFAVVADAVRTLAQKSAEAAKDITSLIKDSVEKIEK
                     SSEVADKSGLVMQDIVSSIKKVSELVSEISSASSEQTGGIQQISQAMNQLDQGAQSNA
                     ASSEEIASTSEEIASQATQMQQLTEKLNEVVLGTNDVAKATSSKRIKTQTKQNIAKFT
                     LKKKSSKNKIAAKESIPFGEDQSVGDASGF"
     gene            complement(37997..39058)
                     /gene="mltG"
                     /locus_tag="VIG33_11390"
     CDS             complement(37997..39058)
                     /gene="mltG"
                     /locus_tag="VIG33_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY8271482.1"
                     /translation="MKKVTLIVLIAVFGLVISTGATVGYLAHTFLTSSPSVESTEVIY
                     EVKPGQSFNAVAKELEANGLIRNAYAFSIYARIMNLRHQVKRGEYGFRRNMVPSEVLA
                     VITSGKSIAKPFTVAEGLNIYEIADLYERQGFGIKSEFLSLAKDKAFAQQLLGEPADS
                     LEGYLFPETYMITKFDSAHDLITSMVKRFLTVYNEVGASQALPGWSRNQIVTLASIVE
                     KETGAKNDRTLVSSVFHNRLEKHMKLQTDPTILYGIADLTGVMPNNIRKEDILKPTRF
                     NTYVIGALPPGPISNPGKESLLAALKPTASKYLYFVSRNDGTTMFSTDLGGHNKAVQK
                     FQVNAKSREGKSWRDINKK"
     gene            complement(39055..40557)
                     /locus_tag="VIG33_11395"
     CDS             complement(39055..40557)
                     /locus_tag="VIG33_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HEY8271483.1"
                     /translation="MEIYPIKYQGDFIAGRFVPVEKGDGEFKDVSPGDLNDQIMSVKF
                     KHDHIDEACLAAQKAFSSWARLTFDQRKSYLFRIKDIFEAHTEQMAQLISRDTGKSLW
                     DSLTEAKNLGSKIDITINHSLRLVNETHIPNALPGVEGVVRHKARGVMAVLGPFNFPA
                     HLPNGHIIPALISGNTVVFKPSEQTPAVGQFMAQLIEKAELPAGVFNLVQGDGESGRR
                     LAANENVDGVLFTGSYEVGLRIKQETINHYWKILALEMGGKNATVVWDDADMEKAVYE
                     TLIGSFLSTGQRCSGTSRIIVHDKIADEFTEKFYQAAKKLTIGHWSENPFMGSLINAA
                     AVEKYIRFQEIANREGCESMMRGKSLDLKYKGHYVTPSIHLVNKFDPNSVYQKSEIFG
                     PNVAIYKSSDFDQTLDIVNSTGYGLVMALFSKKRELYEESLVKARVGLLNWNRTTNGA
                     SSRLPFGGMGKSGNDRPSADYAIQYCTVPVASLEDPTPFDKTKMLPGISL"
     gene            complement(40557..41594)
                     /locus_tag="VIG33_11400"
     CDS             complement(40557..41594)
                     /locus_tag="VIG33_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HEY8271484.1"
                     /translation="MSFIIRAVRHDDLTQLVDLAKQFSLLNLPGDRKVLSQKIDRSEQ
                     SFAGKLPKAQAEYLFVLEDTEEKLIVGSSLAIAKHGTEEVPHCFFKILKRDMFSQDLG
                     VGFIHQVLRFQLDNDGPTEIGGLLVDKVYRRRPEKLGKQISLARFLYIGLHPERFESR
                     VLCELTPPLTEEGRSEFWEALGRRFTGLPYQEADMLSQSHKEFIESLFPKDDIYLCLL
                     DGKARLVLGRVGEATKPAQHLLESIGFEYLDEVDPFDGGPHYGADVKDILPIKLGKRV
                     KLQEFKDAAFKEQGLLGTDGEDFRAALVSYDLRGKELAIPRKLREALGAEIGDEVFIS
                     PLNYSKRKEQT"
     gene            complement(41591..42937)
                     /locus_tag="VIG33_11405"
     CDS             complement(41591..42937)
                     /locus_tag="VIG33_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY8271485.1"
                     /translation="MDFIEACRKFISIDSTPAHGNKEIAEFAAKLCQQQGLHVELQND
                     FVGDVEQVNVIARPQAERAPLEFLLQTHLDTVDPGPFPLWTQAECNPFNATIMDGKIY
                     GLGTADVKLDFLCKLEALSSFGQGKTWKLPPVLVGTYGEELGMQGALRLIRKNKITAK
                     MALIGEPSDLNLIGAGKGFASVEIRIPFSLEEIEYRQEHNLRESTSTQSKLFIGKAAH
                     SSTPHLGESAVIKMLQYLQQLPDSVTVMEIDGGVNYNSVPSHALLEIDIVSNSTKPIV
                     AKIMGIFEAVKLLEKEFSKYPDNDFYPAEPTLNIGLIRTYPDHVLIAGSCRVPPIISN
                     EIYESWMEQIREVCEDNGSQFRITDYKKPYRTKETSDLVKGCCEELKNMGLPASLTTQ
                     PSTNEASIFSRTGIDCLCFGPGKREGNVHTPSEHVMVEDLYKAVDFYKRVLERFCL"
     gene            complement(42948..>43743)
                     /locus_tag="VIG33_11410"
     CDS             complement(42948..>43743)
                     /locus_tag="VIG33_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8271486.1"
                     /translation="NPAYKVGLPEYHEILRVPFYDKFAANSGEKALNALKEHVAKHGK
                     NISAFSFEPMLGEGGYQAAPRDFFVPLLDFCKTHNIAVWADEVQTFTRTGELFAFETL
                     GIGQYIDICTIAKTAQVGATLYTEEYNPKPGLIAGTFSGSSVALSVGSEILDMLQEGF
                     LGPEGRIMQIHKQFISKLNELNETTCKGKVQDAGGMGLMVAFTPFDGKKEHADALTKK
                     LFNNGLISFICGKDPVRIRFLVPAIIKNEEIDLAIKIIEKTILEGV"
BASE COUNT        12404 a        10242 c         9055 g        12042 t
ORIGIN      
        1 tatagagaga ttcagttctt caatggttaa gacttgaggc tcattgcttt gaaccaggtc
       61 cgtttgcagt aatggtttta attctggctg atcagacatc taagagggaa cttaatgatc
      121 ccttcctaga gagtcaaata aacattttgg attgaccaaa ggcccccaac aaaaaaacaa
      181 atccacgcga gtcccttgtg acgactcacc tgctggaaag ctgcgcccag acgctcctcc
      241 ccaatcttta cgagaactgg attttccagc acacctacta aaaggagtcc atagaaaaaa
      301 acgacaaatg ggaagaaata atccaatttt tcgactgtca tgattcgctt atcggacaaa
      361 atcgtcttag catgagtctg gcctgattgc ttttttttga agtatctata caatagagtg
      421 aaggttcgac ggcgtaagac ttgatgaaaa taaaccttat aagcatgtat tgggggctgt
      481 ccctgacaat ggtgtgcaag ctcagctcgt actgagaagc ctaaagttgt taccatggcc
      541 acccacctta gcaataatag gtttatgttt caaagttgcc ttcgagcctt ttttttactt
      601 tttaaagctc aaaaaaattt tccctcaatt ttctgattta ttctggctct ttcacttctg
      661 actaaaaccg caacagtttc cgcaaaatca atctgacatt tgtggattat ttcttgacct
      721 attttgagac aggccattta gccccccatc aaaaggcccg ataataaatt catggactta
      781 agaagtggct cgatccagct ctgctggtac cttattggct tttcactcct cgcattaggg
      841 tgccaaagct caatagaacc agataacact gctgcctatg ctcaaaatcc cgaggcgagt
      901 tattgtggct ctgcccaggc tagaacttat tcttcaaata ccacaacaat ttcaggtaca
      961 gccaccttca cttacaggcc tctacaaaca gcaagtaatt gcggcctttg cggagatcct
     1021 gtctccctcg gagttaccta tgcggaagtc cacattcttg atagctcagg caacgttatt
     1081 caatgcggag aaaccgatca actcgggacc tttagcctga tgattccacg ggtggctggc
     1141 tcttataaag tgcaagtatt atcacgatct ctcacggata aagttaaagc cagcattctt
     1201 aaagatattt attccgccac tccttatacg gtcgaaaccg cttttagcct caactccacg
     1261 caaagcaccg cttcgggtat ttctgtcgtt gcttccgcgc gagcaagcga tgatagcagc
     1321 attcccggtg gcgcttttaa tattctcgcg aacatttttt gggcgaatga gttcataaga
     1381 gaaaactcta gtagctcaaa ttttgtcgct gacaaggttt ctgtcttttg gaaggccggc
     1441 tttaatccct actcttactt caatgctccg agtgtgttag cgtcttttta tgtaaacaca
     1501 gaagatcgac tttatatttt gggcggaaaa aatgatgatg tgaaatcctc tgatacagat
     1561 cactttgatg actccgttat tttgcatgaa tacggacatt ttttagaagc tcattatgga
     1621 cactcagata ccccaggcgg ttcacacaat gggaatttta ttattgatcc acgtcttgca
     1681 tggagtgagg gctgggccaa tttcttccaa ggaacggtgc ttaataaaat tgtctactcc
     1741 agcacctaca cgggccctaa aaaaggcagt tccggctttt cttattatat tgataccgtg
     1801 ggttttgcca atgacagcgt cgagggttcg ggttcttcct ataacattca gatctcacgc
     1861 gacttgagcg agctcaactc tatggcatat tatgataagg cggcaatcga cgaaggcatt
     1921 ttccgagaat tctctatttc tcggacactc tttaagacaa taaccaacac cgacggtctt
     1981 cctttcgcag ccatttggga ttcctttaaa gagttgagaa atagccactc accggcttat
     2041 aagtttgtaa attttggact ctttaactct attctgcaaa cacttctcaa cgcaaattat
     2101 tcaagcctag atcaatcaac ttggaataca gtgatcggcg aagaatatca gaaaaagaat
     2161 acggatctgt atgggagata tcttggcggc actcaagccc cctctggcgg cactccggcg
     2221 tgcccagaaa taatcatgga tccagtgatg gatagcatat atacttatga tcgttcaaat
     2281 cagttaaagt ccaatgactt tttcaccttc tatcacgatg gcacaagtaa gcaaatgggt
     2341 ttgaaagccc ggtctacggg taatgtcatc gacatgaatc tctacctcta tgccgatggc
     2401 tacaactatt ccgaagaact gcaagaggct caagccggct ttaacaatac ttctttgctt
     2461 agaaagagtg ctactttaaa caccgcttcg agcacagaga cggaagttat agactttagc
     2521 ggcctgccag caggctatta catggtgaac gtaaaagcct caactctgaa taaaacatcg
     2581 gccaatttaa acggtggaac cgcgaattat tatttatact ttggcaacag cacaacggtg
     2641 acaacatgtc taataccaaa ctaattctat tttctaaaat gacgttgctg ggagttctgg
     2701 gtttgtgcct aggcggaacc ggaatgtttt attatcaaaa acaacatcgt attcaccgtt
     2761 cggttgcaag cattgaacct tttcaacccc atggcctgac tcttggaaaa caagcggcgg
     2821 caatgacgat tgaaatcgtt ggtcctgaaa actatccaga taacaattct gaaattgttg
     2881 aattggtggg ctatatcact caacatcttg cggccaatag ttcgctggcc tatgagtgga
     2941 gcttgcccga gggtgtggaa cttatgcgag gacccctaag cgacaccctt ggtaatttac
     3001 aaatggggcg accgcggcaa gtcagtattt tagttcgagg gttttctcga gaaagacaaa
     3061 agttaatttc cttgagaact caagtgattg caggcaatgc ccccctcgtt gcctccgcca
     3121 cgatcgtcag tcgtcctgaa gacacgatgg aatccaaagt catggatctg caagtccaag
     3181 ctaaggcggc ggcacaggcc gctaaagaat ccgaaactga atcgagataa tttaagatta
     3241 aagtttaaat aggccccagt tggattctaa ttgtgaattc aaaataattt tgtctccatc
     3301 tcactctgag agcccagtct gaatctcctt cttcctgttg agcacttagc tccacgcttc
     3361 atactgcgac atgttcgcga cttaagtcct ggtaaggagc atccgaaacg atatttagta
     3421 gaacgtaata ggaggtattt atgtttcgtt ttcgcaatgt tttgagattg tcttgcgctc
     3481 tctttgtcgc gggatcaatg gtaggttgct caaaatccgc aaaagatgat gcttcgagtt
     3541 cgacctctct ttcttcttca ttaattagtg aactgtcaca ggctctgggc tctgtaagtg
     3601 tgtcctcgtt aggacaacaa aagatgatga aatcgagcga atttcacaca ctttcaactc
     3661 ctctcacgga tgttcaaatt caggcgattg tcgattcagc caagaccgct gtcgaatccg
     3721 ccaatttgaa ctcttcggat gacctcattg cgcttcttcc tgtgattgcc gcagggacag
     3781 aagataaact tgaccagatc aacatcccga gcgcttcaga taaaatcgct atcattgagg
     3841 ctatcagcga aagtcttttg acctcgatgt caggtcgcga agctaatctt cccactggat
     3901 cagcggatat gggactgact ccagcggaaa ccgctgtcaa taaattatca agtgccatca
     3961 tcagtcactt tgccgatgcc ggtctggaag cgacggatat ccccaccgct tcagaggcgg
     4021 cgatgaatac aatgattgta aacctcggct ctgcgggaat tcctttagcc ggtttggatg
     4081 gtgctgttaa acaaatgacc gctggggctg tcagtggcct tgataatatt agcgtgagtg
     4141 gttttagcgc ctcaaacttg ccaaccgtgg ttgcaagcct tactgcaggt gccaccaaag
     4201 gattaggaag tatcagcacc tcagggtatg attccagcaa acttccggac atggtgaaaa
     4261 atgtggctgc gggagccacc ggcgccttgg gatatatttc catgactggt tatgataaaa
     4321 acaaccttgg acccatggtg aagcaaattt caggaggaat cgtcggagct cttgatggaa
     4381 tttcaatgac cgggtatcaa gcttcctcat taccttccat ggttgaaagc gcttcagccg
     4441 gtgttgtggc caatcttggt tccatcaaca tcagtggcta ttccgcaacc gatgtttcaa
     4501 gtcttgcctc ggcagccacc gccggcgcta caggggcctt ggataaaatc gttatgaccg
     4561 gatatgatgc tgattccatc gcgacaatgg ccgcaaaaat caccaccgga gccacaaagg
     4621 gaatggggaa tatcagcatg acaggatttg atgttaccaa ccttccagta gtcgcaaaaa
     4681 gcattacatc tggggcaact ggcgaactcg gtaacattgt tatgtcagga tacgatgcca
     4741 cgaagctgcc aacgatggtg gagaaaatct ccgcaggtgc aacgggcgct ttgggtgaaa
     4801 tcactatgac gggttatagc agttcaaacc tcaatgacat ggtcaaacaa atttccgcag
     4861 gcaccacagc agccctcgga accatcaata tgaccggcta tgacgcaact tcacttccga
     4921 agatgatgga gggcatcacc tcgggtgcca caggggcttt gggctccatc tccatgacag
     4981 gctatagcgt gagcaatctt ggtgatctgg caaaaaatat ttccgctggc gcaacggcaa
     5041 gtttaggcga tatcagtatg acaggctata gtgcgaccaa cattactgag atgatcaagt
     5101 atgcttcctc aggagcgacg caagcacttg gaaacatcac catgcctggc tacgacaact
     5161 ctaaaattca aactatggtt cagtcgattt ctcagggttg tacagattca atcggaagca
     5221 tcacgggtct gggaacaatt aatgccagca cagcaattca gtcattggca gagggcgtat
     5281 cgctcggggc tcaaggaatc gctaccgttt atccgactgt gatcaacacc tccaatctgc
     5341 aaagttggat tcagacagga gccagcacca cggcgaatgg ttacaccatc ccgaacctca
     5401 cggccagtgt gaataccgga tacaatgccg atccaatagc ttccttcgtg aattttatgg
     5461 ataccaatac ctcccacgga attatcgcag ggcatatcgt gattggtgca agtatgaatg
     5521 aaaccgctgt aacttcttac cgcgtctatt ttggtaccaa tccaacgaca aaaacgggtt
     5581 cggcgatcac cactcagact aagtcctccg gagccaattt ggactacacc ttggccaaca
     5641 ccacagtccc cacggaagcc actcacctct tggtttatgt tgtgaaaaca gacacaactg
     5701 agacctcagt gcccgtggcc attccattga tggatttgtc atccgcaggt gccaccgtca
     5761 acggagcggc aaatgtgatg ttgaataata cttatactcc tgtgggaatg atcaacggaa
     5821 agcaagctta tatggccaat ggctacttca tcttctggga atccgggtat tggatactcc
     5881 acagttcaaa cacccttacg atgccaggca tggagactat gacatcagcg tcaaaactcc
     5941 agatggtgca actctacttg ccttcgacag catcgacgcc tccgagtaca ggttggcttg
     6001 tggggtataa tggcgccagc tcaacggcca cagtcagcgc taattaaagg attagaaaaa
     6061 tagccgaggc ttgatggcct cggtgtcagt ttaaagcgcg atcacttcaa tgctgggatt
     6121 gaattcgtcg cgaagaattc cttcaatcgc catcaaagtt ccagtggttg cactgggaca
     6181 agttccgcag gccccttggt agaatacagt caacttgttg tcctcataac tgaccacatc
     6241 caaatcacca ccatcccctt gaaggcccgg acgcacggtg cgatccaaga tctcttcgat
     6301 gtgttgcatt tctggagatt gattttgacg acgcattttt ctctcgtcga attgagtttg
     6361 atttggatta tgaaccgcca tgcgtgcttg aatcaccgcg cacactgctt tttggatttc
     6421 atccgagtcc gcgtcaaagc tatgcgtgac cgtgatcaca ttttgaaaaa agtgaacttg
     6481 ctttaccccc ggaacatcga aaagacccgt cgctaaatga ttctgagcag cttcttcaat
     6541 atttgaatag gtggctttcc cttcattcaa aaccgggcgg tccaagacaa atttccaagc
     6601 agcgggattg ggagtcgctt gggttctaat taatacatct tgtgcgttca ttgaaataac
     6661 tcctgagctt ttttaacagc ctttaaaaag ctctcgacat cttgcatatt attataaact
     6721 gaaaaagacg cgcgcacagt tccgctgatt ccgaaacgtt tcattaaagg ttgacagcaa
     6781 tggtggccag cacgtacggc aattccttgt tggtcaagga tttgtcccac gtccgaatga
     6841 tgcgcaccct cgagattaaa gctaagtact ggtcctttat gagccgctgt acctacaatt
     6901 tttacttgag gaatttttaa aagaccttca gtcgctgcgc ttaaaagctc ttttttatgg
     6961 gcttggatcg aatcaaaacc aatactctta atgaaatcca tcgcggcatg cagggccacg
     7021 gctccttcta cattcggagt tcccgcttcg aagcgaaaag ggatctcatt gtaggtggtt
     7081 tgctcaaagc tgactttaga aatcatgctg ccgccgcctt gataaggcgg catttgctcg
     7141 agcagttctt ttttcccata aagaatgcca atgccgaacg gcgcaaataa cttgtgcccg
     7201 gagaaagcaa agaaatcaca atccaaagct tggagatcca caacctcttg cgccactgcc
     7261 tgcgcaccat cgacaaaaac tttcgctcct actttatggg ccgcttcagt caaaaaacgg
     7321 atatcattta tcgtgcctaa aacattcgaa gacgcggtaa acgcgaagat ttttgttttt
     7381 gctgaaagct tcaccatgaa atcctcaaga tccagctctc ccttgtcagt caccgcgacc
     7441 gcttgaacct tgactttctt ttctgcggca atcatctgcc atggaacaat attcgcgtga
     7501 tgttccatct cagtcagtag aatttcgtca ccttctttaa gaagggtccg ggcgtaggtt
     7561 tgcgccgcaa gatttatggc ctcagtcgtt ccccgcacaa aaatgatttc ctcggcggat
     7621 cgggcattta aaaacttggc cacagccgat ctcgcattct caaagttcac cgtggctttg
     7681 tcggccaaac tgtgagctcc gcgatgcaca tttgatattt catactgatt aaaattataa
     7741 atacgctccg ccacagattg tggtttcaaa gtggtcgcag cgctatccag atacaccaaa
     7801 tctttgccat tcactttttg cgtaagggct ggaaattgtg atcttacttt ttcaaaaaac
     7861 gaatcttggc tcatattatt ttaccgccat gtcaagcccc gtaaaggctt cccgaagctc
     7921 tccattcaac caattctgta actcttcatt ttctaattta taaatcaatt ccgaggcgta
     7981 gccgaaactc atcatcggaa tagccgtttg aggggaaatt ccacggctct ctaaataaaa
     8041 taactcttca cgatccagtt gccccaccgt cgatccatgg gcggctttaa cgtcatcagc
     8101 gtaaacttca agttgaggct tgctgtcggc ttctgcctca cggctaagca agagattatt
     8161 atttaactgt tcagaattcg ctttcaaagc cccaggacga attattactt ttccattaaa
     8221 cactccacga gaagcccccg ccaaaatgcc tttataatgc tgagtggtgc tacaagcgcc
     8281 gacttgatga tccaacaaag tcgtattgtc cacgtgctga tggcctttgg ctaaataaac
     8341 cccatccaca gtggcaaaga cctccaccgc tttcaactcc atctcgagat tgtgtcggga
     8401 aagaagcgcc cctgttgaga aaacaagact gtgtaaatga gccgctttgt ccagatgaaa
     8461 agttgtacgt cccatatgaa tggcccgttg gctgtcctct tgaactcgga tcgcggttag
     8521 tttcgcggct tctccaactt caatctgaac ctgagagttc acaaaataac gagcctcttt
     8581 ttgtccgaag taagactcta caaatttaag tgaggaatgt tttccaagct tcaaagcaaa
     8641 gcgaggattt atcatcactg cgggtccccc ttccagggag ctataaaaca ccatattcac
     8701 aactttatcc acagcggtgc ctgcagagat ttcgagctta tagtttttag tgaagtaaac
     8761 cgtgcttaga gcctcaaaag aatctttgaa atcccgagcc gtcaattcct tatcttgctt
     8821 aagtttcaca cctgtgggaa gagtctccaa agctgaaaga gttttattta aaacaccatt
     8881 acaaaagacg atgttaatga aagctggatt taatttttct tgaattctcc gcatcgtctc
     8941 aggagaagga ttcaactcgt tgaattcagt cggcacaaaa gtttcatcat ttagaacttt
     9001 tacactggta tacttccagt cttcatcttt acgggtcggc agacctttta cgttcaaaaa
     9061 ttccaaagcc ttctcgcgca aagcctgaac ttcaggctct ggtgctggat tttgctttgc
     9121 aaacttatca aagtgagtaa gtaaattcat aattatacgc ctaccaacca atcgtatcca
     9181 cgctcttcaa gtttttccgc caaggtataa tcacccgttt caatgatttt tccgtttgct
     9241 agaacatgga cgaagtccgg tttaatataa tgaagtaatc tttgataatg cgtcactaac
     9301 acgatcgcat tgtcctttcg gcgcagttta ttgacgcctt cggaaacaat ccgcaaagca
     9361 tcgatatcca ggcccgaatc cgtttcatcc aacaaagaca aacgcggtga tagaaccgcc
     9421 atttgcaaaa tctcattgcg ttttttctcg ccgcctgaaa atccaacgtt gaccggacga
     9481 tccaaaaaat cttttttcat caggaccaac ttcattttct cttcaacata attgcggaag
     9541 tccgcctcat tcatgggctc cgaaccttga tgagtcaaaa ccgcattaaa ggcggtgtgc
     9601 aagaaattga aattgctgat ccctggaacc tccaccggat attgaaacgc caagaagatg
     9661 ccttctttcg ccctttcatc cgcagccaga tccaaaagat tgaccatatt gaagttgatc
     9721 tcgtacttca cttctccctg agtcactgta taggctggat ggcccgctaa aactttagaa
     9781 agcgttgatt ttccagaacc gttgggtccc atgatcgcgt gaacttcacc ggctttcacc
     9841 gtcaggttta aacctttaag gatttctttt tcttcaactt tagcatggag attcttaatt
     9901 tctaacattg tattacccaa ccgaattttc taatttcatt tcaattaact tcactgcttc
     9961 taccgagaac tccaaaggca attccttgaa gacttctttg caaaatccat tcaccaacat
    10021 ggagatgctt tgctccatac ttaaaccccg ggattgaaga tagaaaatct gatcttcact
    10081 gatgcgcgag gtgctcgctt catgttccaa agtagccgtt ttgtttttga cctctatata
    10141 gggaaaggtg tgggcactgc aatcagggcc taccaacatc gaatcacact gagaatagtt
    10201 tcgcgcattc tccgccgacg gcatcacttt gacttgccct cggtaagcat tggaagaatg
    10261 ccccgtggaa attcctttag aaataatcgt gctcttggtg ttttttccaa tatgaatcat
    10321 cttcgtgccg gtatcggctt gcatatagtc atgggtcagg gcgaccgagt aaaaagctcc
    10381 ttcagaagag tccccttgca aaatcaccga aggatacttc caggtgatgg cagatcccga
    10441 ctccacttga gtccaagaaa ttttagcgtg cttaccagcg gctttgcctc gcttagtgac
    10501 gaagttataa atgccacctt tgccttcttt gtcacccgtg taccagtttt gaaccgttga
    10561 atacttaatc tcagcttctt cgagagcaat aagctctacc acggcggcat gaagttgatt
    10621 ctcatcacgc tgaggagcgg tacaaccctc cagataattc acaaaaccac cttcatcgca
    10681 tactaaaagt gtgcgttcaa attggccggt ttctttcgca ttgatacgga aataagtcga
    10741 taaatcaatc ggacatcgca ctcctttagg aatataacag aaagagccat cagtaaaaac
    10801 ggccgcattt aaagcggcat aatagttgtc cgaaaaaggc accaccgttc ctaaatattt
    10861 tttcaccaac tctggatgtt tgtgaaccgc ttcagagatg gagcaaaaga tcactcccac
    10921 atcttcgagt atttggttat gagttgtccc cacagaaacc gaatcaaaga cgacatccac
    10981 tgcgatccca gaaatacgtt tctgctccgt caggggaata ccaagttttt caaaagtttt
    11041 aataagttca ggatccaact cgtccaaaga tttgggacca gttttttctc cctctgaagc
    11101 cttcttcgga gccgagtaat aacaaatgtt ctgaaaatca attttcggat atttcacatg
    11161 agcccacgta ggctcggtca tcgtcagcca ataacgaaaa gcttttaacc gatattctaa
    11221 catccactcg ggttcatttt tcttagcaga gatcaagcgg atcacatcct cattgagtcc
    11281 ctttgccacg gcgtccgttt ccaggtccgt gacaaaaccc catttgtatt ctgtatccgg
    11341 aagtgtttga accgctttag gattattatc cattgaacac atcctctgca tttacgtctg
    11401 cagtcgccat cataggcact ttgcgctttt ggtttttcac attacctagt gaagacggtg
    11461 tttctatcaa aagctctttc aaagtaatat tgctatagaa ctcattgagt ttatattgca
    11521 gaagctgaac gggagacaaa atattgcaag tgccctgaat ctcgcaggga ctttctttat
    11581 gcatgcactt caccatcgtg gtgggaccct cgataatttc cagaagttgc agcatcgtca
    11641 cttttccaag atccttggtg atttgatagc ctccaaaagc cccttgctcc gcgcgcaaca
    11701 aacctttctg agccatggcc tgcatgacac gagcggtggc atcaaaagga gtggcgaagc
    11761 tttcagagac ttcttttgct gaagtcagct ctccgggaat cttgtgactc atatgcttta
    11821 aagccatcag ggcatattct aatttgcgat tcagtttgtt cataagcacg tttccttaaa
    11881 tcgtattgca agctaaatga aacaattcca aaactcaaga actaaatacg cctttttttg
    11941 gcgtatatga caggcttttt aagaattttt aaataccaag taaattcgaa tgcttagacg
    12001 taaagttaat aaactgtaac cgattcattg ttgattccat ttgggctata aaacgcccta
    12061 tattatgaag atgcttcaat acaagcttcg tgatagacca ctgatcacta tgagagttct
    12121 atttattagt gctgtatcca tcattgtttt gtttattgtc ttattgattt ctcgcctttg
    12181 gggaatgggg caacaatatt ctgaattcca tcatcctttt tttgaaggtg caccctctcc
    12241 cttgattatt gttaaggtga atacagaaaa agcactgcaa gaagctgccc agcaaaaatc
    12301 cgatgtcgca ttttggttgg atgtggaaac taccaatgat cagaaaatca ttgtttttgc
    12361 tcatgacatt caggcaaaag aaatgtcgat tcaaactttc cgtgggccta agcctttagc
    12421 gtaccctttc caacagttgc acaggatgcg ccctgaagtt cgtgagctca agcgaattct
    12481 cgctcaattt ccgacgcaga aattcgttct gaatatttta gacaatgtga cgggaattca
    12541 taccgggttg tcagaaaatc tcaaagggct ttccccagaa aagcgtattc ttatacagtc
    12601 gaattacaac gtcattttaa ccagcctgaa agagatagaa cctttttggc tttatggctg
    12661 cagcaaagcg gatcttatga gatttctcac tttcgaatcc atgtgggtgc tgccagcgac
    12721 tccctttaaa ggggacgttt atattgctcc attctttttg gtcggacggc cagcttttca
    12781 tgaaagcact ttggaagaag ttcgtaggcg caataaaaaa atcatcttag gcgctgtgct
    12841 ggataaaaaa gaatttgatg atgctctacg tctgaaggcg gatgggatca ttgttgaaaa
    12901 tttcggtgat tatttaaatt ggtctagacc atagtcttat attctgggtt gcgaacagga
    12961 cgttttggtc ttagcctaag aacctatgaa tatacctatc ataaatattg ccaatgccgc
    13021 ttctaatact caagttgcga ttaataccag tccgatggat gctttgactc aagcaagtcc
    13081 tgtggttcaa ctgactttgt tgatcttagt aagtctgtct atcttctgct gggcgattgc
    13141 ttactccaaa tacaaacagc tccaacaagt tcgggccgcc aatgaacatt tcaccactaa
    13201 gttctggaaa gtaaactccc tcgatactct ttatggagat atcgatgaat ttcccttcag
    13261 caatatggca cgcatcttta aatccgctta tattgagatg aaaaaactcg cggattcccc
    13321 cttagtcaag aatagcggag aggacaagcc ccaactttcg ggtctggata atctagagcg
    13381 agcgattcgt aaatccacag aaaatgaagt ggcaaagctt gaaacccgtc tgaccgtcct
    13441 ggcgaccacg ggaagtacgg gacccttcat tggtcttttc ggaaccgttt ggggaatcat
    13501 gggatcgttt cataaaattg gcgcgacagg aaatgccagc ctagccgttg tagcgcctgg
    13561 aatttccgaa gccctgatcg caaccgcaat tggtttagcg gcggccattc cagccgtcat
    13621 tttatataat aactttgtag gccttgttcg ccgtgaggaa attgagctca ataacttcag
    13681 tgcggatttc cttaatatcg ttaagcgcaa cttcttcaaa ggtgactaag tatgggaatg
    13741 agtaatggtg cctcttcaaa gagccgcacg actctcagtg aaatcaacgt cactcctttg
    13801 gttgacgtca tgctggtgct actcatcatg tttatggtga ccacacctct catgcagcag
    13861 gggattgaag tggatctgcc aaagacttct tccgcaggag tagagcttaa cgatgaaccc
    13921 ttcgtgttgg tgatcgatgc agctcagaag atgacggcgg gtaagcaaaa tattgttatg
    13981 ggagatttgc gcccgaaact aaaagccatc tttcaacacc gtaagaataa acaggtctat
    14041 attcaagcgg atcgaaaagt ggactacggt ttcgttgccg aagccatggg cgagattcgc
    14101 gccgctggtg tcttcaatat cggtttaatc acggttccaa aagataaata aacagctaat
    14161 gaaaaggcac aatgaaaagg taaatgagtt ctaaacagaa atctctcatt caagacaaag
    14221 atctcgctgc aggcctaaag atttctgtcg gtctgcacgt cgctttggtg gcggcatttt
    14281 ccattcgtgc agccttcttc gaaccggaac aaatcgacta tacagccgct gttcgcgtgg
    14341 atatcgtggc tctaccggat aaaagacccg atcacgttcc accacaggct cccgcaaaac
    14401 aagaagaagc cgcaaaagca gagcctaagc ctaagactga gccaacaccg gtggggcccg
    14461 ctcaaacctc caagcctcct ccgcaagagg taaaatctaa ggttccaaaa gacagcgaag
    14521 ctattaatct cgaaggaaaa aaggcccaaa aagaagctct cgaaaaattg aaggccatgg
    14581 ccgccattga agaacttaaa aaatctatgg agaaagaggc ctctaaaaaa atagccggta
    14641 tgggtgaggg aaaaatgcaa tctaatacag ttaaaggcaa tattctttcc cctggcacgt
    14701 ctctcacggg tttgaataga cttcagcatg atagttattt agcgggccta gaccagcaaa
    14761 ttaagcagaa ctggttttta ccgcagtggt tatccaagaa acctttgcgc actcaagttc
    14821 gtctgaaaat cgacgaaaaa ggtcacattc tttcgcgtga aataacttta tctagtggca
    14881 atagcagtta tgatgaatta gctctggaga caatcgataa atcggcgccc ttcgcgcccc
    14941 ctccggaaaa gtttgtatct atcgtagccg tcaacggttt actgatcggc ttccctgaat
    15001 aaataataga aggagtattc catgctcaaa tttctgatag cctcaatgat tacggtgact
    15061 gcactttcct tccccagcgc tttcgcgcaa caggaggcgg ttcgcgaacc ttcagccgct
    15121 acttccagcg cagcctctga tattaagctc ggagaagcac acacgaaaaa aagtcttttg
    15181 gcatttcccc cgctcaatta tcagggtagc cccagtggct cctatcaggc aacaggagcg
    15241 gagttataca acactataaa caatgacctt aatgtctcag ctttttttca aatgattccg
    15301 caaaaagcct atttagagga cacatcaaaa acaggacttc gcccggctcc tggagagccc
    15361 aatggtttta aatttcaaag ctggagcaca gtgggagctg aattcctgat ccgtacggga
    15421 ttctttatag caggcggtga agtcaatcta gaagcttacc tttatcatgt aggtaaatcc
    15481 caactcatta tggggaaaaa atataagggc cctatttcag gagtgcgcaa gattgcccac
    15541 acttttgcaa atgatgttgt agaagctttg acgggtaaaa caggaatgtt tctttctaaa
    15601 atcgtcgttt ccagcgataa aaacgtcggc cgctttaaag aaatttatat catggactgg
    15661 gacggagcaa atccagaaaa agtctcaaac catcgttcta tttctttatc ccctgcttgg
    15721 tccccagatg gaaaaaaaat tgcttacacc tcctacgtgg ttcgcacaaa aacaaaaatg
    15781 cgcaatgcgg atatgtttct ttacgattta accactggaa aacggaattt gatttctttt
    15841 cgccaaggtt tgaactccgg ggcctgcttc gctccagata ataaaacaat ttacttaacc
    15901 atctcccaac aagggactcc ggatctctac aaaatgagtt ttgatggaac ccttgaaggc
    15961 aaaataacca atggccctaa caatgccatg aatgtggaac ccgccatcag tgcggatggc
    16021 actaagctcg ctttctcctc agatcgcagc ggacgtccca tgatttacac aacgaacccc
    16081 aatggttcag atgccaagcg catcactttt gccggggtct ataactccac tccctcttgg
    16141 tctccggacg ggaagaaaat cgctttcgcc gggcaaagtg aagaccattt tgatatcttt
    16201 gtcatgaata cagatggaac aaatatgata cgcctgactt ccgcgaaaaa gcccaacggc
    16261 agatggtcaa ccaatgaaga tcccagtttc tctcctgatg gacgctttgt tatgtacacc
    16321 agcaaccgca ctggcaaaag ccaaatattt atctcaaccg tggatggatc cgaagaacgt
    16381 cgcgtaactt tggacaatgc caactatttt aaacccaagt ggtctaataa cattgagtaa
    16441 tttgaagtgt cctttaaaac ctctttcttc ttacacggcc tcagagattt ctgaggccat
    16501 aaagttttct ccggcacttt cgcattacct cggttttacc tttcttcatt cgcaaactcc
    16561 ataccatgaa gtgcgcacca acgcttggat tctctctgcc ttagctacaa ttctgaattc
    16621 ggaaaacccg gggaagattt gcaaaacctg gagtcacgtt gccaatgata ttcttcaatc
    16681 tgtttttcgg gagtgtttca aggattcagc gattgctctt tttgcgttgg gaaaacttgg
    16741 cgcccaggag ctaaatttaa gttcggacgt cgacattctg gttgtttcaa aagaggaaaa
    16801 tgtccatcac ttgaaagctc ttcgtcagtt ccaaaaggta ctgagcgaaa gaactccgcg
    16861 gggctatctt ttcagagtcg atttcgatct acgccccggg ggacgacaag gtccgctgat
    16921 tccgactttg gatcagtttg aagattatta cggaaactac ggagaaacct gggaaagact
    16981 cgcctttgtc cgcttaaccc ctatcgccgg agactccaac gttatttctc agaccctgag
    17041 ctttgctaaa aagtttagct atcgtaaaca cctcgattat actctgttgg aagatttgaa
    17101 atccctgcga agaaaaattc gccagcatta tgctccgaat tcaacagagt ctgtgtggga
    17161 tttgaaactc ggagtcggag gcattcgcga tgtggagctt ttcgtccatg ccatgcaggt
    17221 tattcatggc ggaaaaaatc caaaactaca agttcacccg actgcggatg ctctggcgct
    17281 tttagaagaa acggaaatcc ttccgaaggt ggatgcacaa tttttaaaga atcactactg
    17341 gcaactgcgc caactagaaa actttatcca ggcgcaaaat gacgaacaga ctcatctctt
    17401 agaaaaaacc acccctatgc cggagtgggc ggagtcgatt cttactcatc ttaataagga
    17461 ctttgagcat tgcgatcaca tcgtaagttc cctgctagga aaagcctccg ctacaagctc
    17521 cgccgccttt aacgtagtgc cattacaaaa cacaaaatcc tgggaagttt ggcgggaaat
    17581 cttgcaaata gaagtgctat cgcgaaataa agatcgggat gagcaagctc gtctgcaatt
    17641 tttgaatgaa ttctatgaaa ccttacagac ccaaaaaggc gacaccgaca aggcgctaca
    17701 acacttaaaa gaattcataa agtccacccg ggcaaaagcg agcttcttta ctttattgat
    17761 caggaataaa aacttaatgg atgaactggc ctggcttttt ggccactctc cctatctttc
    17821 gcaaattctc agttcgcgtc ccgagcttat tgacagctat gtctatcgct ctcaagaatt
    17881 tcaaaagggt gacctcgcgg tgctcttaga acagttggta gagaagcgtc ttttgggtga
    17941 gatcatcaat ggctctcatt atttagaaga caaaaacctc gaggaattac aggccaatct
    18001 tagcgccacc gccgatgaaa tcgctggaac tcttttggcg gagctgaaaa aagaatatcc
    18061 ctctgaactc gaaattctct gcctcggaaa atggggcggc aaagagttgg gattccgctc
    18121 ggatttagat tttattttca tcacacctca cgagcccacg gaaaacgata gcaaagttgc
    18181 aagacgtttt atcaatcgcc tgaccgaaac ccgcaaaggc ggaagtatct atgcaatcga
    18241 cacgcgattg aagccctcag ggaaggccgg tccctttgtg atctctgaat cccagctcct
    18301 cacttacctc cacgacgaag cgcaagcgtg ggaacgccaa gcctatttaa aaagtcgaaa
    18361 actgaattct gggataagct caacccctca acaaattctg aactcttttg cgaataagaa
    18421 gctcaccgag accgaactcc aggaacttga aagaattcgt gaaggcctca tccgctatga
    18481 atccacctcc ttcgacctta aatatgcgga ggggggaatg ttggatattg agctgttttc
    18541 acaagccgaa atattggctg agtcgctcgc cataaggggt cccagcactt tggattttct
    18601 ctctgtcatt gccaattccg acggtcttca acgtaattac ctacgtctgc gtcagataga
    18661 acaaatgttg caattggtat cgacggaggg cggcgcaaaa gtggtgttaa atcatgaatc
    18721 atttcagcag cttgctaagg cactccagct gtctgcagaa gatttaaaaa cagagtttaa
    18781 ttcactaatc tctgagaatt tacagatact taatcaactt gaccggcgtc gacgctcaaa
    18841 gatacttaaa gcagctacat agagcatcta cttaagacga ctacttaagg cagctatttg
    18901 aaagtttgac tcacaatagt tttttaaagg agttggcaat gatgaatcgt tctcacctta
    18961 atgttagtac caggaatgta aatgttaaga aggtattgtg gatatatatt ttggcgtttg
    19021 ttttgaccgc ctatagtttc cactccaaag ccgcagagaa aaagaccact aaagaaggaa
    19081 aagggaaaga catgatcgca gtttttgaaa caaataaagg cacattcaaa gttaaacttt
    19141 tcaatgaaaa agcaccaaaa accgtagaaa catttgttgg cttagctgat ggtacccgcg
    19201 agtggactga tcctaaaagc ggcaacaaag taaaaaaacc tttctatgat gggttaaaat
    19261 tccatcgcgt catcccgaac ttcatgatcc aaggcggaga tcctcttgga actggtactg
    19321 gtggccctgg cttccgtttc gaagatgaat tcccagcagg ggctccaaag cacgacaaac
    19381 cgggcatcct ttcaatggca aacgcaggcc ctaataccaa cggctcacag ttcttcgtaa
    19441 ccgtagcggc aactccttgg ctcgacggca aacacaccgt cttcggcgaa gtcatcgaag
    19501 gcatggatgt agtgaccaac atctccaaag taaaaaccgg cgccatggac cgcccagccg
    19561 aagacgtcgt catcaatcac ttaaaaattc agaaataggt acgccgtacc tttttcggat
    19621 gcgccgcaaa acccacgttt atagcgtggg tttttttaat caatccatgg aacagtaata
    19681 tctgacttaa gctctttcac gggttacctt tttatagatt gaactataaa attctcatag
    19741 agcttaattt cgacgttcga aatttacgtc gaaattaagg gatcgagtta agatcggttt
    19801 gagcttgaga gagattgctt tcgatgtctt tccaatcggc gcgaacctct cgtaatttaa
    19861 tgcggatgtg atcaagatca gaccagagga ccgagcgacg gccacggatg taggcggctt
    19921 cctcgcagat gctgcgaatt tcattttgct tagtggcgtc gttataaacc gtgatggtat
    19981 ggcactccgt cgcattgctt ggcaatgtag catactcacg atggatattt gtttccgttc
    20041 tagcgtagct ggcctcgagt tctttgaccg ctacgagctt agagttcaat tcattgatgt
    20101 aattagtcag atattctttg cgacccttat aatacccagg caagaaagct ttctcctgct
    20161 gaggactgca aaggttctgg tacagctttc ctgccttggc gaagctatag cctttttccg
    20221 ccgtacagta agtaaccaag ccttgctgat agagactcct catacggttc atatcaagtc
    20281 catcgcaaaa tttaaaaaag accgggatcc cttctttagc acgggtaaaa atctcgtcgt
    20341 actgacacat tttttcgcgt ccctgtttaa agcctaaatc aagttgcacg gcactcgtgc
    20401 tatcgatccc tttgcaggct ttaatcaaac catcttcctc aagatatttt ccttgataag
    20461 ccacattctg tgagtactca aaccagttgg tcttttcaca tttgtcttgc aatgcccaat
    20521 tcgcgcaacc taccgtgaaa agaactaaaa ccaaactcaa aattttatta aatctgtgac
    20581 tcatcgtaga tcctgttcct caaatcttac ttttaaatct tatgttttac agataaaccg
    20641 cctcggctgt catccggata acacctcgca gacaaattag ggcaggcttt cgcgaaatct
    20701 caatttgaga caactcaact ctaatatatc caggcatcaa gtttgcttct tcaacaatca
    20761 agcggcggag tctgcttagg agacatcatg aagtggtcca aaatcattac ctcttattta
    20821 gtgattgcac agctagtgct agccccggta ttaagccatg ctctcaatgg gcagctagac
    20881 attgacaata atggacgact caccgaagag ggaaagtcca ccgttgctgc ggctaaaaat
    20941 tacgcaaaag aaaataagct caaaggtcgc ttggatcttg ccaataaaaa aataactttt
    21001 aacgatccaa agactaataa agtagtcgga gaaatggccc taagtgatga gaaagctctt
    21061 ttagagtatt ccccaaaaac attgaatcaa aaactgatga aagaattggt caaggtcaaa
    21121 gccgccaatt ccgctgcttg gtctcatgcc acaagatctt ttgccgggga aagcgcgatg
    21181 ttttttctcg tcttgggtgg aatggtcggc ataaagctca tgcatgatta tgccagcgac
    21241 ccagttgcta tgaaacatca tattgagcaa cagctctctc ctgtcggcca aatggccttc
    21301 tttatgttta tgtattccca aggtgttacc gccaacacct taagcctctg gattaaaaac
    21361 ccaaagctcg gtttacccat tgccatgctg ggtatgacag ttggttctgc ggttcaaact
    21421 tatttctcgc aagtggcagc cgatccgcat attcgaaatt gcgccgcctc tgtttttaag
    21481 ggcgaaaaaa tcgaagggaa tgatcacccc tgtgaaggag cctataaata cttagtgata
    21541 gataaaaaga ttctcgaggg tccgggcgtc attgcgctgc ttggagcctt tgttgcctca
    21601 accggaatcc gtgtggcttt aggagccagc atgaaactgg taggattcga aattggaatg
    21661 tggctcactc ctggcggtat ggccatgaag ttcgctcgcg ttttattcag tgccgccaac
    21721 attaccagct tcactctttt tcaagcgaaa atagaacatt tcgctacagg cctatggaaa
    21781 aattactatg atggctggga gttcgtagaa atcaatgacc gcctagtctc ccacatcgaa
    21841 aatcaaaaag cctcccagtg gaaatctgaa gctaaagatt tcaataaaga agtgagagaa
    21901 ttttcaggga aaatgaagga gtggcgaatt accaatcttt cagacgccta caccgccaac
    21961 caatcttggg gcgaattttt aaataacctc agctccatgt attcggcttc ttacagtttt
    22021 tatagtacct accttgatca tctgaagggc aagaactcct ttattgaccg cccctatcca
    22081 cttttcggcg tcattcccaa agggcttgtt gcaggtcacg aagatcttta cctgactcat
    22141 ccacaccgca ttgagtttat gcaaattgaa acaataaatg atgtggctgc ttggattgag
    22201 caaaccactc aaagtggcta ctataaaaaa ttagggttct ttaaaaatca agaggatatc
    22261 atttctaaaa tccagcaagg actggccagt gaagacatat ccaccaaagg tacggcactt
    22321 cttgagctta ataaagtaat tgcgaaagat gctaaaacag taacgggttc tctcttcggc
    22381 cgtgaacttc gtagaatcca ttctctgctg ggcaagccct accctctttt ggaaaaaggc
    22441 cgcggttttg cgtcaagcat gttgtatgcc ccaagtatga tctccgcttt cggggatgct
    22501 aaagcggatg attccaacgg acattttagt acgccaaata ttacggacta tctagttgtg
    22561 caaatgatgt gtggaccaaa tccttcaaaa ggagaaaagg ttgtcagcgt tacgcagggt
    22621 tttcctgcta aatttatggc gccaacaatc gctctcgatg atccctctaa aaaagatctt
    22681 tgtactggca ctttcctgag tcgggtacga agtgaacgta ttttcaaaga catgcctttt
    22741 tcaaattacc ccacggcccc tgacttctta agagccaacc tgaatcccgt agtcgctgaa
    22801 gactttatct catggtggga aaaaggtaca gagacacaga tgaaagaggc ctacaaaggc
    22861 ttccgtgtgt ctttcaaaga ggttctaagt aagctcttta aaggcttgaa ccaaatacag
    22921 aataatcctt ggaaccgagg atttatttcc aatggagcta tcccttcggc cttccaagaa
    22981 atgagacttt acgctctcat tttaggagag ctgttgaaag attcctacaa agttcagtat
    23041 gaaagagatc ttcctaccag ttattacagc gataaaatcg atcctcaatt gcgtgcgacc
    23101 acctccgatt atttgacaat gagtaaaaag ccgcttttag gactgctcgg tcgtggcagc
    23161 gtttatagct tcaatgccct cgttggggcc gaaccaaaca cgaaagaggt gcatagcctg
    23221 aagattcaga aagagctgga gcttcttttt tctcagatgg atggtttagt tcaaagggct
    23281 aaagctcatg tcgtaaaatc tgaagaattt gaaaatcaaa tgaaggctat tgagaaaaaa
    23341 ttgactgaat ttggcgcatt gttaggcgtg agcaaagacg gcgttaatat ctttgcaaag
    23401 ccagacagtg aggctaaaaa ttcagacagc ggagaggaga ctttggctcc agcacctcgt
    23461 ccagaaccca gcaccttatt gaaaatcacc aaagactcat ccgcttccag tcccttttta
    23521 aaacctgaag tatctgttcc atcccacgat acgctctcag gaagcgataa aaatggtccc
    23581 aatcagggac tcgttacttt ggacaaagag caaaaggcgc tcgctgtcac ctgtttagaa
    23641 cttttacagt cggtggccca ggaattatcc atgtatggcc atatggcggg aacagccctc
    23701 tttgaagaga aaaagcaaga ttaaataagt ggaatgaata ttgaaataat tgaaataatt
    23761 gaaatagcta ttaaccgtaa caacgaacaa agcggcaaag gagtattaaa atgaaaaaga
    23821 agtttatcgt agggatgatg atagtaacta gtaccctgtc tttcagcgca catgcggccg
    23881 ggacagcagt cgaagctcgt cctgccaggg aagttttgaa gacctatgtg gaagacgtta
    23941 aaaagtgggc tgctaaaaaa atcggagaga acagagcatc tcttcccgtc ggggaaaaga
    24001 tgaaaactga gaaagctgcc gaaatcttta tggaagaact taaagttcca gcagccgaca
    24061 aaacatcgat taaatccgct atgggaaaat caaaaaacaa aaaagcgttc aatgacagtt
    24121 tagccacaat catggccact agaagaatga ccgaaggaaa atcagatgtt gaatccaaat
    24181 cgctcgaaga agcttctgat gctctcatcg gagtcttagc agacgctaaa gatattggtt
    24241 cgacagcgaa ttctaaattc tttaatgaga aaaccaccga tttcgcggat acaacggctg
    24301 ctcttaataa agtggtggaa atgcctgata agttcattct tttcgataca aagacgcgag
    24361 atcaatttac taaagccctc gtaaagacac gcgaactcag ctataaatcc gaaacttatg
    24421 aagaagcttt cgtcaaagct ttgatggaat cacaaggtat tgatagggca aaagcgatgg
    24481 aaatcgttcg taaacttaaa gactgtgttt agtatttata atggtttaat aagacctcgt
    24541 gcggaagcct agcaatgact tccgcacggt tcattattaa gtgctagttt actttctcat
    24601 cccccatcac gataagaatc tcaatacggc gattttttgc acgattttcc ggagtgtcat
    24661 tcggaactag gggcttctga tccgcgtaag aaatagcggc catccttcga ggatccaagc
    24721 tttgggactt aatcaaatat cgaaccacgc ttgtagcacg aagactcgcc agttcccaat
    24781 tgctggccac ggactcggaa ctgataggtt gatcatccgt atgaccttct accaagattt
    24841 ttttatctgt ctggcggagc aattctccga tctgacccaa gatcttcaac gaagaacgct
    24901 tcattttaaa ttccccttca ggaaatactg atgaagcagc caaggagacc cgaaccccca
    24961 gggcatcgtg atggacttct aagcctagtt ccttttgagt cacggcattc atctgcttac
    25021 ctaggtagcg agtggcataa tcggcgacct ccgcattgct gcctttttgc ggaaagctct
    25081 gcgctccgcc attctcttgt ccatccttgc tttgaatata ttcatcaatg atcttgctgg
    25141 catccggcgc ggcacttgtc ggcttctcct cagcagcggc accaatccct aacctaagat
    25201 tggcccggac cgagttttca aattctttct gtttgccgag attatttgtc gaagtcgcgt
    25261 acataaccac aaaaaaagca aaaagcagag tgataaagtc agcgtaagaa accaaccatc
    25321 tttcatgatt ttcatgctct tcatgacgtt tcctcattcc gcctacttta gctcgtgggc
    25381 taaggccctg agttttcgat ctatgatcat cgtgcttaaa gaggaggtga tcaacagtcc
    25441 gccctcaagg aggactcttt tgtctttcat tctttgcgcg attttcttct taattttatt
    25501 tcccagggga ataaaaacaa gatttgcaaa acccactccg taaatagtag ccacgaatgc
    25561 cacggcaata ccagctccaa gtttacttgt gtcagaaaga ttacccatca cgtgaatgag
    25621 gcccaaaacg gcgccgagga taccaatggt cggagaaaag ccaccggcat cgctccaaat
    25681 tttcgcggct gataaaagtt catcttcctc tgaatccaac ttattttcga atatttcttt
    25741 gatggttgcg ggttcgattc catcaatgac agtccgtagc atttctttaa taaacggatc
    25801 tgaagcctta gaaacttttt cctccagcgc catcacattt tctttacgta caatgcgtgc
    25861 gtaatcgacc atttcattaa ttttataatc gatatcgctt tctttctctt ggaaagcctg
    25921 gctcgctaat tcgaagccaa ttttaatatc tttcgtatta tttgaaacca tcacggcgcc
    25981 cagagtcccg gcaatgacaa tcattgcagc ggttccctgc agcaacgaac caaattggcc
    26041 accatctaac gtattcgcca ataaaatacc gccaatacca atcagtaatc cgaaccaagt
    26101 cgctttatcc ataatattaa tatggtgcca taagggccca ggaataacac gggcgcagct
    26161 ttgttcctag actttcttag gaggagaatt tactttggga aatatgagtt ttctgtacga
    26221 gctggacaaa agccttcttt taaaaatcaa tcaagagtgg acctcttcag gggctgatat
    26281 ttttttccct gccatcacag atctgcacaa gaacccctgg ttttttgggg ctatagcaac
    26341 gactttgctt attttattcg tctggaaata tagaaaggtc ggcttagtat tcttcgtggt
    26401 tttattactc gctctgggga gcactgacct cattggcaac cacattttca agaaaaacgt
    26461 cgcacgcgct cgaccaggcg atgagccctc agccatggtg atcgttcgct ctccttatgg
    26521 gggctatagc ttcacctcaa atcatgctgc caacatgttt tgtttggcaa aatatacaag
    26581 tgagtttata cctcaagtgc gtttgattac ttattctgcg gcccttttga tttcgtatag
    26641 caggatatat aatggcgttc attacccttc agatgtcctg ggtggtggaa tcatgggttg
    26701 gattgtggga tggatctata gtatcctcgg caagaatatc tacaacagac tgagaaaaag
    26761 agttgtatca tgaaagttct agtcacagga gcgaacggat ttttaggaag ctggctaacc
    26821 caagccctcg tcaaacaagg acacgaagtc tttgcgttag tgcgtgcaca aagtaattta
    26881 tcagaactga aagacgtggc ctgcaactat gtttatggag acatcacgga cctcgaaagt
    26941 ctttataaat cctttgccgg aatcgacact gtatttcatc ttgcagggct tattgcttat
    27001 aaaagaagtg accgcgccaa aatggaaaaa attaatgttt tcggtactga aaacgtaatc
    27061 gatgcttgtt taacaaaaaa agttagaaaa cttgtctttg tttcttcagt tgtcgcagtg
    27121 ggtgcgggct ttactcctga ggaagttctc catgaagagt ctccctataa catcgaacat
    27181 ttgaatctgg gatattttga aaccaaaagg gcggctgaga aactcgtgat aaatgcctat
    27241 aaaaataagc atttagattg tgtgattgtc aatccaagca caatttatgg caaaggagac
    27301 gctaaaaagg gcagccgcac aactcaagtt aaggtcgctc aaggtcgctt taacttttac
    27361 acctcagggg gcgtcagcat tgttgccgtc gaagatgtag tggctgctat tattacggct
    27421 tggaataagg gacgaagcgg agagcgctac attttatcag gtgaaaatat aaccatcaaa
    27481 gaccttttta caatcatcgc tgaagaggct ggagtacgtc ctcctcaaca taaaatgcca
    27541 acctggatga ttttcgcatt gggccatctc ggagattttc tggagagtct aggcatcaaa
    27601 ggcgccttga gcacagagaa cgccagaacg gccacgctct accattggtt tgataacagc
    27661 aaagcaaaaa aagagttgaa cttcaacccc cacacagccc gcgaagcgat ccgcaatagc
    27721 gtctcgtgga tgaaagaaaa tggattgttg agctcaaaat gaaaaagttt tttaaaagat
    27781 ttgctttcac ctgtttaata attttaacag gggtgagttc cttcgcggct tatgtgtttt
    27841 ataaaattcc ctcagaaaaa gagattaaag gctgcttggt cactaccatg tatcaagtca
    27901 atctttgtcc cggatccaaa gattacgttc cgctaaaatc catttccaca tacttgcaga
    27961 aaacggttgt tcttactgaa gattccgctt tctttcagca tgatgggttt gactggaaat
    28021 caatcgaaga aaatgcgaaa aaaaatctag aaaaaggtca ctatgcaaaa ggcggttcga
    28081 cgatttctca gcaattagcg aagaacatgt ttttatataa agacaaaact ctgcttagaa
    28141 aagggcttga ggctttaatc acgacgaaaa ttgaaaagac ccttacaaaa aaggaaatcc
    28201 tggaacgcta tctaaacgtt gttgagttcg gtaaaagcat ttatggcgtc aagcaagcgg
    28261 cgaatttcta ttttaagaaa actcccgccc aattagatgt tgttgaatcc gctttcttgg
    28321 cgatgatttt acccaatccg atcaaatatt ctcagtctta tttccgcaaa gaattgactc
    28381 cttttgctca aaagagactg aagcgcattg tgactgatat gtatcagtac aaccgtatta
    28441 ctgaagatga atacaatgtg gccattgccg aagtcagtag tttcttctca ccgaatccca
    28501 tgcctgaaga cagtaacgtt attgaatcgt cggaccctac cctggaggaa cttgaaaatc
    28561 tcgggaacga tgaaggcaaa gataattctt ttttttgagt ggggactgcg atgattttta
    28621 cttcagcaat ttatggcgaa ttctataaat agcaatattc ataagcagta ggccgaggac
    28681 tagcaacaca agcacatgaa tgctcaccgc agttgtgaag ccctcttgga gcaggccacg
    28741 caccgcttga actgcatggg tcaatggaaa caaataggct aaatacttga ggccccctgg
    28801 aagctgctcg ataggaaaat aagttccact caacaatgac ataggaacaa taaatcctga
    28861 ggttgaataa ataaaggaat catagtttcg tgcaatggaa gtcataatca ttgcgattga
    28921 agagaatagc cagcatagta tcaacaaaac cgctaaagcc ggaaaaattc tccaggtgtc
    28981 tatgaggcca aaaaaagccg caactaaagc gacaccgcag acactgaaat acccttttgt
    29041 acaagaccaa agaagttcgc caaaggcgat ttcctcagga ccaattctag tcaacatgat
    29101 ggtcgaataa gttctttggt gtgtcagttt tgtgaaaccg ccataggttc cttcgaaaaa
    29161 agccaccatc attccggtag aacacaaaag gcctggaaag aaaaagtcga cataagaatg
    29221 cccgttgata ttactgacga aagcgcccag tccaaatcca atggccccta agtacatcaa
    29281 aggttccagt acaacccaaa ataaagaaac cgtccaggtc ttacggaagt atagaaaatt
    29341 tcgagaccac acccttataa aaccactgtt tattttcggt ggacgaatta tttctacgaa
    29401 tttcattatt catccctcaa tgcatgaccc gccaatttta aaaacacatc attcaaagtg
    29461 ggcttgcgaa taagaatttt atcgctggcg atcagatcaa tcgctttgcg tccttcttga
    29521 ttgggattta taagaatcat cacagtatcc tgaatgactt ggtaattata gccaccctcg
    29581 cggagacggc caatgtagta atttaaatcg acaggattgg tttcaaactc caccacttcg
    29641 ataccaatat gctccagaat caaatcagcg ggcttaccca tcgccaaaat acgtccgtga
    29701 tccataattg ccacgcgatc acacaaacgc tcagcctctt ccatataatg ggtagtcaac
    29761 acaaccgtgg aattttccac tttcaaatgc ttaataaaat cccaaatcca caagcgagcc
    29821 tgaggatcca atcccgtagt cggctcatcc ataaaaagaa gctctggact gtttaaaagt
    29881 cctcgagcaa tagccaaacg tcgtttcatg ccaccactga gagtgtctac tgagcgatca
    29941 gcgtattctt caagccgcat ttctcgcaat aacaattgag ctttcccaga agcagctcga
    30001 gcagggattt cttgataact tgcatagacc agcagattct caagaacact gaaatctgga
    30061 tccagcccat cttcttgagg aatcactccg atgcgagttt taatttcacg ataatttttt
    30121 ttaacattaa gacctaggac ataaagttcg ccactggtga ctaaagcact gcaatacatc
    30181 attttcatcg tagtggactt gccagcccca ttgggaccca gcaaaccaaa gcattcccct
    30241 ttgggaattt ctagattaat gccgttgacg acaaagttct cgtcgtattt cttcttgaga
    30301 tctttgagtt caacggcaat cttctggctc attattctct cgctgctttt ccacgacggc
    30361 ttgaatcgag ctcacggcaa cggatgcgaa ttgtcttagg agtgacctca atcaattctg
    30421 aatctttaat ccattccatc gctctttcca aagtcatagg acgaactgga acgatgcgaa
    30481 tcgcttcatc cgttccggaa gcacgaacgt tggtcagctt tttctcgcga gtgatattca
    30541 ccacaagatc gttttccttc gcatgctcac ctacgatcat accttcaaac acttcatctc
    30601 catggttcac atacatcact ccgcgctctt gaagattcca aatagcgtat gcgacagcca
    30661 tgcccttgcg gtcagaaatc atcgcaccat tcatacgatg ttcaatctca cctttgaagg
    30721 cttcataacc atcgaactgc gtgttcaaaa gacccgttcc ccgcgtatct gttaagaaag
    30781 ttgatctata cccaatcaaa ccgcgagaag gaatgcgaaa ctcgagacga gtgcgaccgc
    30841 tgcctttttg aaccatgttg atcatgatac ctttacgttt accaagtttc tcggtcacta
    30901 cacctacgta aggatcctca atatcgatga ccgcgatctc cataggctcc gttttttgtc
    30961 cattctcttc tttgaaaata accgttggct tagaaactaa aagttcaaag ttttcacggc
    31021 gcatctgttc aatcaaaaca cccagttgca attcaccacg accgatcact ttaaatgaat
    31081 ccgtcaaatc ggttttttca acccgaatcg ccacgttgta aagaagttct ctttcaaggc
    31141 gctcaatgat tttacgagaa gtaacgttct ttccttccat gcccgcgaaa ggtccgttat
    31201 tgaccgagaa gatcatgccc accgttggct catccacgcg gatacgcgga agaggaatgg
    31261 gctctgtcac agaagtgatg gtatccccga tagtgaagtc ctccatacct gcaataacca
    31321 caatatcacc ggcaccaact tcttgggctg gaacttggga attcactttg tattggaata
    31381 aagcggacac ttttactttc ttttgtgcat gctccatcac acacagaact tcatcaccca
    31441 ctttaattgt tcctgatctc atacgaccga tggccaaacg acctacgtaa tcattgtaag
    31501 aaatattgga aaccatcact tgaagtggcg cgttctcaac aatttttgga ggaggaacaa
    31561 ggttgataat agcatcgtat agaacctcaa gagaccctgt gtttactgcc ggatccaaag
    31621 tggccatacc ttcacgcgca atcgcataaa cagtgtggaa atcacattgc tcttcgttcg
    31681 cttccagatc gataaataaa tcgaaaagtt cattatgaac ttcctgaata cgagcatctg
    31741 aacgatcaat tttgttgata cacacgatga ctttcaaatt ttgttcaagg gcttttttca
    31801 atacgaaacg agtttgtgga agtggacctt cagaagcatc acaaagaaga atacaaccat
    31861 caaccatatt gaggatacgc tcaacttctc ccccgaagtc actatgtcct ggtgtatcga
    31921 cgatatttac tttaatatct ttgtaagtga aagacgcatt cttcgccgcg atagtgatac
    31981 cacgttcttt ctcaagatcc atagaatcca tcaaacgctc ttccacgtgt tcgttttcac
    32041 ggaaagtacc tgcctgtttg atcaagtgat caacgagggt tgtcttacca tgatcgacgt
    32101 gggcaataat ggcgatattt ctaattttct ttggatcctg aatcattctc tgtctttcct
    32161 tatgtgtctt taaccgtgtc cacccatgag gaactcttca tggctagtgt cttcacgttc
    32221 ttcttgttct attgttccaa tacttttaaa aaactccatc gctagcccca tgagttcatc
    32281 attggatttg gtttggaata aatttttacg aaattgagag gcaccaggat accctgttga
    32341 gaaccaagct gcaaactttc gcagctgaat tcccacgata tactcatcac aatgcgccac
    32401 aatggcctcg cgcaggtcca taaaaaggcc cataaagtcg cgtgaaaact gctgagaaag
    32461 ttcttccccg cgccacaaac ttaaagcatc cataaaaata tgtggatttt ttaagcaacc
    32521 tcgaccaatc aggactccat cacacccgga ctctttcatt cttgtcacgg cctgctgagg
    32581 agtcaaaatg tcgccattcc caaggatcgg caaccgagct tttgctttaa tttcagtaag
    32641 ataatcccag ttggcttgtc ccgagtatcc ttgggcacga gttctcccat ggatcgcaac
    32701 ccaagtaatt ccctcattgt aagcgatatt gcaaacttca tgggcattca gtgtgtttgc
    32761 atcccagcct gtacgaatct tgatagtcac cggaatcttc accgctttca ctacagtacc
    32821 gaggactttt tccatcaggg gtaaatcgcg gagcataccg gagccagctc cttttttaac
    32881 cactttagga actgggcaac cgaagttgag atccacaaag tcacagccca tcgcttccgc
    32941 tacctgagct ccacgagcca gaatttcagg gtcttccccg aaaagctgga taccaatagg
    33001 tctctgagtt ttatcaaagc tcattagctt taaagtccgt tcgcttttat actcaattcc
    33061 cgtcgcactc accagctcag tcaccacaac gctggctcca cgctttttca taaacgtgcg
    33121 gaaagcgtga tccgtgattc ccgccatagg ggctaacaca aaaggatttt gttttaaggc
    33181 ttcaagtgga tgcatacgaa aggtcagggt atagcaaaag accgcctctg gtgctagcgg
    33241 aatcatgtta agttattatt ttatttgaag tttttacttc gtcgtccatt tgtttataaa
    33301 ttgactcaaa ctcgcttgat atacccttgc ctcagattca ttataagccc ttaaagttaa
    33361 tttttggagg acctatggca gatgatattt ttaaaaaacc cctcgagacc ccctatttag
    33421 agccggcact tgaatgtgat ttcggagctt tcgctaaagt cgtagaaagc cggcgatccg
    33481 tcagagtcta tactgaggaa gtgatccccg actccgtcgt ggaaaaatgt ctggatatgg
    33541 ccttattggc accgaactcc tccaatctgc aatcttggga tttctattgg gtgaaatcgc
    33601 cggagaaaat gcaaaagctc aaagaatatt gccttaatca gcctgcggca aagacagctt
    33661 cgactctaat tgtggctgtc gcgcggcccg atgactggaa gcgagggcaa aaaattaata
    33721 cggactattt agctaagcta aaaaatccgc ctgcaagtat gaaatcttat tatgaaaaac
    33781 tcgttcctat ggtttatgga aacggcccct tgcatttgct cgccccgata aaaagtcttt
    33841 tgtttgctgc catcggactt ttcaaagtga ttccgcgagg acctttcgga agatctggaa
    33901 atcgtttatg ggcgacaaaa accaccgccc tggcttgtga aaacctcatg ttagctttta
    33961 gagcggcagg ctacgatact tgccctatgg agggtttcga tgaggttcgc gtaaaaaaac
    34021 ttttgcagct cacttacgga gcttccgtta ccatggtgat cagcgccgga aaacgcgcac
    34081 ccaacggagt ttacggagag cgccttcggg gtcccaaaga aatgttcgtg aagatcgttt
    34141 aaggtaaagt taattaatga tcacttcaaa aatttgtcca ctgccaaatg tatcaacaat
    34201 atagaggctt aattgtgaat gggtcacctt cacaattaag gaattataat atcctagatt
    34261 ctgaatttta aagaacgacc cattcgatgg aacaatggtc atgaattggg agggatcata
    34321 ggaagagcgg ctcgtaagct gagccagaat ctgctccgtt ttaagtagcc gttctgacat
    34381 ctcaatggat tctgctgtgg ggattatcgt gctgtattca gaatgaggat ccttctctgg
    34441 tttattgtac tttgaagtga gccgattatt taaatattgc tgtaaaaatt ttggccctgg
    34501 atgtaaatca atatcagaat cttgaatatc ggccttacca tttgcaaagc tgccatataa
    34561 aagaacgtag tcgtccttaa aataaggtgc tatcgagctt ttttgaatgg ctttttgcct
    34621 gagtgaagtg aatgaaacct cttgttgaaa gacttgctca caaataaaag gctttacttc
    34681 cccaaaaacc ataacaggga gaagccaact gaaagtcata taaacaagtc ttattttgag
    34741 attcactttg ctcctccttt gctttcaaca ttaatcaagc aagcgttata ccaacttttg
    34801 ttatccacaa acccagccat ttgatttgag ttactgaaga aggggataag gcgtaatttg
    34861 taaagcaaag aaaaccgcct tgacgttcca acttgaaaca tttcttgttg tcaccaataa
    34921 taggatggat gaaccttctc agtggtcttc tttcaggcat ccccggcgcg aagctggcgc
    34981 tcccactgtt actccaacca agtcacccgg ccttccacct gacgaggggc tttaggaggt
    35041 tgatggtctg gataacctat gaccagtcca caaagtaatt ctaattcacc ctttttatat
    35101 tcaggaagct ggtctacgcc gaggaagcgg tccacctctt ctttgatctc gagaggcgcc
    35161 cccattgtgc aacagcctag gccttcagcg aaacacgcaa gattcatatt ttctacggcc
    35221 atatacacac tgcctatact cgtgtgatag cgctcttcat tgtcattgtg ggaatatgca
    35281 aaaataagca ctggagcatc gcctagggtg aaaaaaaatc tctcggtgaa atcataaagc
    35341 gaaggtttca agcgctgttg aaggacgttc ttaatcccaa gccaagactt ttgtgaatac
    35401 ttgagatatt catctcgttt ttttccttga acgacaaaaa atcgccaatt ttgtctgttt
    35461 ttccccgaag gagcttccat gcctgcggct aagatacgtt ctagcacctc tcgaggaaca
    35521 ggatcttttt tatatttccg aatcgatctg cgaaacttta gcaaattgta aaattcttct
    35581 ttattcatcg tgttcattct aacaaactta tgccagactt cacctatgga atcgaaaaaa
    35641 aatacaatct ctgctgaaaa tctcttcagt ccattccttg gtgtcctgat ttttctggct
    35701 ctcctggtca cggccactgc agtcacgccc agctggcgtc agaaagtaag ggatttcttt
    35761 aatcctgaac aacgtaagat tttggctaaa acctccggcg atcttactgg cgaagggctt
    35821 caaatcacag ttcttaaaat aaaaacgcga gatcatttag ttttagaagt ttatagagcg
    35881 gaaagtccag attcgctgaa cttgatagct cgagtggact taccagaaaa aagagacgca
    35941 tactttcaat taaaaggaaa tgccacgaat ctgggtctcg tagacgtgga taacgatgga
    36001 accttagaaa ttgtggctcc ttcttttgac gagcaaatga ttgcaagact gaatatttat
    36061 aaattcaatc ctgccactca aagtttcgat cgcctaagtg cgccagcaga aactgagttt
    36121 taattatatt tatctagcaa taaagtgaac gtccatatct ggcaaagcct gaataaatcc
    36181 tgtaagatcg gcaggtcttt taagtattgc ctgccgataa aatttttgct tttaaattaa
    36241 ttaaaatccg gacgcgtcgc caacagattg atcctcacca aaagggatgg attctttagc
    36301 agcgattttg ttttttgaag attttttctt taacgtaaac tttgcgatgt tctgctttgt
    36361 ctgagtctta attcttttcg aagatgtggc tttggcaacg tcattagtcc ctaaaaccac
    36421 ttcatttagt ttctctgtca actgctgcat ttgagtcgct tgcgaggcaa tttcctctga
    36481 agtcgatgca atttcttccg aggaagcagc attactttgt gcgccttggt ccaattggtt
    36541 catcgcttgg ctgatttgct gaattccacc agtttgttcg gaagatgctg atgagatttc
    36601 gctgacaagc tcagacacct tttttataga ggaaacaatg tcttgcataa ccaagcccga
    36661 tttgtcggca acctcactgc ttttttcaat tttttccact gagtccttaa tcaaactggt
    36721 aatatctttc gcggcctcgg cgctcttctg cgccaaagtt ctaactgcat cagcgacaac
    36781 agcaaagcct ttgccctgtt ctcctgcccg cgccgcctcc actgccgcat tcaatgccaa
    36841 taaatttgtt tggaaagcta tatcatcaat aacatgaatg atctcttcga ttttcttaga
    36901 cgcatgagaa attccccgca ttgattccac caaattctta atttctcttt ctccattttc
    36961 ggcggattcg cgggacgact gcgccaagcc tgccgcttcg cgggcatggt cagaattcat
    37021 ttgcaccatt gaagtcattt cttcaagaga cgctacagtt tcctctaagg aagcggccga
    37081 ttgagtggat gattcggaaa gcgcatgacc agcggcggat aactgctcaa tggcctgggt
    37141 cacttgaccg gtggaatctg taagctgagt agccacctga cctacggagt tggaaacttg
    37201 ataagcaatc cacataagaa tagaaaagat tgtaagaatc gcgaaagctc cggtaaataa
    37261 aagtatattc tggctaaagg agcccaactc tcgctgttct ttgtcgctta cttcgacttc
    37321 cttgatataa gattgcgaga ttttatcaaa tccttctttt atctgggtgc tcaaagtgtt
    37381 ccactctccc ccggcgttga tattctcaaa agccgctttt aacgactctg ggtcgccttt
    37441 tttaagctga tcaatgatct tttcagtaag ggtatagaac tttggaataa gcaacttggt
    37501 aggagcgaag agctctgctt ctccgggacc gaagggaagc ccttcatatt tttttatccc
    37561 tacttttaaa tcgtcaaagg ccgtctccgc agttgcaata tattttttag agagcaccga
    37621 atctgaagct gccgtgccat aagccgccca aaaataaaat gatattcgtg ggcgtgcctc
    37681 tcgaatatca atgaaaacat taaggttagg gataagcctg gaataagact catttaataa
    37741 gccactaaat ttatgatctg tatgaataga aagccctatc accacagtca aagctacaat
    37801 tggcaagcaa gtcgccatta atagtcgtcc acgtatgccc ttaaaccact gtttcatttt
    37861 ttctcccttt attaacttat taaaataact aacaaaggta tcgacaacgg tccgtttttt
    37921 ctaaagaaga atatttacta aggagaaatg gggcagacct gaagtttgga ttataactaa
    37981 tttattattt atcatgctat tttttattta tatctctcca tgatttgcct tcgcgggact
    38041 tagcgttcac ttggaatttt tgcacggctt tattatgccc cccgagatca gtggaaaaca
    38101 tcgtggtgcc atcattacga ctgacaaaat ataagtactt tgaagcagtt ggcttaaggg
    38161 cagccaataa agactccttg cctggatttg agataggtcc cggcggtaaa gccccgatga
    38221 catacgtatt aaaacgagtc ggttttaaaa tatcctcttt acgaatattg tttggcatga
    38281 cgccagtgag atccgcaatt ccgtataaaa tagtaggatc agtttgtaac ttcatatgct
    38341 tttccaaacg attgtgaaaa acagaagaga ccaaggtgcg atcatttttg gcgccggtct
    38401 ctttttcaac aatacttgcc agcgtaacga tttgattgcg gctccagcca ggaagagcct
    38461 ggctcgcgcc aacttcattg taaaccgtaa gaaatctttt gaccatggaa gttattaaat
    38521 catgggcact atcgaatttg gtaatcatat aggtttctgg aaagagatat ccttcaaggc
    38581 tgtcggcggg ttcacctaaa agctgttgag caaaggcttt gtcttttgcc aaactcaaaa
    38641 actcagattt aataccaaaa ccttgtcttt cataaagatc tgcaatttca taaatattca
    38701 aaccttcagc cacggtgaag ggcttggcga tgcttttacc tgaagtaatc actgcgagaa
    38761 cttcactggg caccatattt ctgcgaaacc catattcccc acgcttcact tggtggcgta
    38821 ggttcataat ccgagcgtat attgaaaagg cataggcatt tctgataaga ccattggctt
    38881 caagctcttt agccacggca ttgaaactct gaccaggttt cacctcatag atcacctcgg
    38941 tgctttcaac actgggagaa gaggttaaaa acgtatgagc caaataaccc accgtcgccc
    39001 ccgtggaaat cacaaggccg aaaactgcaa tcagaacaat caatgtcacc ttcttcataa
    39061 actaataccc ggaagcattt ttgtcttatc aaaaggagta ggatcttcaa ggctcgctac
    39121 aggaaccgta caatattgaa ttgcataatc ggcagagggt cgatcgttgc ctgattttcc
    39181 catcccccca aaaggaagac gcgaacttgc cccatttgtg gttcgattcc aattaagaag
    39241 tcctacacgt gccttaacca gagactcttc ataaagctct ctcttttttg agaagagggc
    39301 catcaccagt ccatacccag ttgaatttac gatatccaaa gtttggtcaa agtcagaact
    39361 cttataaatc gccacattcg gtccaaagat ctcacttttt tggtaaacag aattgggatc
    39421 aaatttattt accaaatgaa tactcggcgt gacgtaatga cctttgtatt ttaaatccaa
    39481 agattttcct cgcatcatgg attcacagcc ttcgcgattg gcaatttcct gaaagcgaat
    39541 atatttttcc accgctgccg cattaattaa agaacccatg aatggattct cactccaatg
    39601 gccaatcgtt aactttttag ctgcctgata aaacttttct gtaaattcat cggcaatttt
    39661 atcgtggaca ataatgcggc tcgtacctga acatcgctga cccgtactta gaaaagagcc
    39721 tatgagagtc tcgtaaacag ccttttccat atctgcatca tcccagacaa ctgtggcatt
    39781 tttgccgccc atttctaagg ccagaatctt ccaataatga tttatcgttt cttgtttgat
    39841 tcttaaacct acctcgtaag aacctgtgaa aagaacccca tccacatttt cattagcagc
    39901 taaacggcgc ccggattccc catctccttg aaccagattg aaaactcccg ctggaagttc
    39961 cgctttctca atcaactgag ccataaactg accgaccgcc ggagtttgct ctgatggctt
    40021 aaagaccact gtgttgccgc taatcaacgc cggaataata tgcccgtttg gcaaatgcgc
    40081 gggaaaattg aagggcccca aaaccgccat tactccgcga gctttatggc gaaccacccc
    40141 ttcaacccct ggaagagcgt tgggaatatg ggtttcattt accagtcgta aggagtgatt
    40201 aatggtaata tctatcttac tgccaagatt tttagcctcg gtcagagagt cccataaaga
    40261 ttttccagta tctctagaga tcaactgagc catttgctct gtgtgagcct caaaaatgtc
    40321 tttaattcgg aagagataac ttttccgctg atcgaaagta aggcgcgccc atgaagaaaa
    40381 agctttttga gcggccaggc acgcttcatc aatatgatca tgcttgaact taacactcat
    40441 gatttggtca ttcagatcac cagggctgac atctttgaat tccccgtcgc ctttttctac
    40501 aggaacaaaa cgaccagcta taaagtcccc ttggtattta atcggataaa tttccattat
    40561 gtttgttcct tcctttttga ataattcaat ggcgaaataa atacttcgtc cccaatttca
    40621 gcccctaggg cttcccgaag ctttcgcgga attgccagtt ccttaccgcg gagatcatag
    40681 gataccaggg ccgctcggaa atcctcaccg tcggttccga gcaatccttg ctctttaaaa
    40741 gcggcatcct taaactcctg gagcttcact ctttttccta acttgattgg caaaatatct
    40801 ttcacatcgg ctccgtaatg ggggcctcca tcaaaaggat ctacttcatc aagatactca
    40861 aaaccgatgc tttccaagag atgctgggct ggctttgtcg cctctccaac acgacctaaa
    40921 accaaacggg cttttccatc gagaagacac aggtagatat catccttcgg aaaaaggctt
    40981 tcaataaatt ctttatggct ttggctcaac atatcagctt cttgataagg aagacccgta
    41041 aagcggcgac ccaaggcctc ccaaaattca cttcggcctt cctctgtcaa tggcggcgtc
    41101 agctcacaaa gcacacggct ttcaaatctt tcggggtgaa gtccaatata gagaaatctc
    41161 gcaagactga tttgttttcc cagtttctca ggacgacgac gatagacttt atcgaccaga
    41221 agaccaccga tttccgtagg tccatcattg tctagttgaa aacgcaaaac ttgatggata
    41281 aagcccacgc ccaaatcttg actaaacata tcccttttta gaattttaaa aaagcaatga
    41341 ggcacctcct ctgtgccatg ctttgcaatc gccaatgagc tgcctacaat aagtttttct
    41401 tcagtgtctt ctaaaacgaa taaatactcg gcctgagctt tcggaagttt tcctgcgaaa
    41461 gattgttcac tgcgatcgat cttttgactg agaaccttac ggtcaccggg aagattcaat
    41521 aaggaaaact gctttgccaa atccaccaat tgagtcaaat cgtcgtggcg gacagctctg
    41581 atgataaaac tcataaacaa aacctttcaa gcacgcgctt gtaaaaatcc actgccttat
    41641 aaagatcttc caccatcaca tgctcagatg gagtatggac atttccttcc cgcttgcctg
    41701 gcccaaaaca caagcagtca atgccagtgc gcgaaaaaat actggcctca ttggtggaag
    41761 gctgagtggt taagcttgct ggaaggccca tatttttcag ctcttcacaa catcccttaa
    41821 ccaaatccga agtttccttt gtacgatacg gttttttata atcggtaatt cgaaactgcg
    41881 agccgttgtc ctcgcaaact tcgcgtatct gctccatcca agactcgtag atctcattcg
    41941 aaatgatcgg cggaacacgg caggaccccg ctattaacac atgatctgga taagttcgaa
    42001 ttaatcctat attcaatgtc ggctctgcag gataaaagtc attgtccgga tatttgctaa
    42061 attctttctc gagcaatttc accgcttcaa aaattcccat gatttttgcg acaataggct
    42121 tcgtcgaatt agagacgata tcaatttcta ataaagcatg actgggcact gaattataat
    42181 ttacgccccc atcaatttcc atcaccgtta ccgagtctgg caattgctga agatactgca
    42241 gcattttaat aactgcgctt tctcctaagt gcggcgtgga ggagtgggcg gctttcccaa
    42301 taaaaagttt actctgagtg gaagtacttt ccctcagatt gtgttcctgg cgatactcaa
    42361 tttcttcaag agaaaaaggg atacggattt caactgaagc gaaacccttt cccgcaccaa
    42421 ttaagtttaa atcactcggt tctccgatca aagccatctt cgcggtaatc ttattcttgc
    42481 gaatgagtct gagagccccc tgcattccta actcttcacc atatgttccc accagtacag
    42541 ggggaagctt ccaggtttta ccctgaccaa aggaggaaag ggcttccagc ttgcaaagga
    42601 aatccagctt tacgtcggcg gtgcccaacc cataaatttt tccatccatg atggtggcat
    42661 taaagggatt gcattccgcc tgtgtccaaa gcggaaatgg cccgggatcc acagtgtcta
    42721 aatgggtctg caataaaaat tctaaaggag cccgttccgc ttgtggcctc gcaatgacat
    42781 tcacctgctc cacgtcgccc acaaaatcat tttgcaattc aacgtgcagg ccttgttgtt
    42841 ggcagagctt cgccgcaaac tcagcaattt ccttattgcc atgagcagga gtgctgtcaa
    42901 ttgagataaa tttccgacat gcttcaataa aatccaacca gactccctta aacgccttct
    42961 agaattgtct tttcaataat ttttatcgcc agatcaattt cttcattttt gatgatagct
    43021 ggaaccaaga atcgaattcg cactggatct ttgccacaga taaaactaat caaaccatta
    43081 ttgaataatt tcttagtcaa agcatccgcg tgctcttttt ttccatcaaa cggagtaaag
    43141 gccaccatca agcccatgcc tccagcatct tgaactttcc ctttgcaagt ggtctcgttc
    43201 agttcattca acttagaaat aaactgtttg tgaatttgca tgatacgacc ctctggaccc
    43261 aggaatccct cttgaagcat atctaaaatt tcagagccca cacttaaagc gactgaagat
    43321 cccgagaaag tcccagcgat caaacctggt ttaggattgt actcttcagt ataaagtgtc
    43381 gcgcccactt gagccgtttt agctatcgtg caaatatcga tatattggcc gatgcctaag
    43441 gtttcaaaag caaaaagttc tccggtacga gtaaaagttt ggacctcatc cgcccataca
    43501 gcaatattgt gagttttaca aaaatccagc agaggaacaa aaaagtcacg tggcgctgcc
    43561 tggtaccctc cttctcccaa cataggctca aacgagaatg ctgaaatatt cttaccgtgt
    43621 tttgcaacat gctccttaag ggcattcaat gctttttcac cgctatttgc agcgaattta
    43681 tcataaaaag gaacgcgcaa aatctcatgg tattcaggca gacctacttt ataggctgga
    43741 tta
//
LOCUS       DATBTE010000306         2874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1132978, whole genome shotgun sequence.
ACCESSION   DATBTE010000306 DATBTE010000000
VERSION     DATBTE010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132978"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VIG33_11415"
     CDS             <1..1243
                     /locus_tag="VIG33_11415"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY8271487.1"
                     /translation="IQSIEPEIYLGSCPLMTLISFEEKVSLPAKLKILQKLRDFFPKT
                     QVVVALQGDEKPEDYDLLKNAGAHYLILSEEIKNSGKLYYLSAVLVQGAYFPVPVTDV
                     FPSTQLSFNAYHKLLLNQKFLPVIFSGFPISDKKYRKLEPSKQIYVRRESLDEYRKYV
                     EAYHDRTGSALKKRCRVVMMNLVGIQCELLLQLTLDVEVRTEISVKEKISRFVQSSLE
                     LGDYLQDCPDVWNVIAHSLDFKFCHYERSIYILAYALYISAKAKLTVLSDLVMTVLLA
                     DLGILELPVECYKTLQSRGEKQLSPQDLEYFHNHPIASLNRLKFHQVELSEEVQSALV
                     CTHERNDLKGFPNQVPGDKIPVAAKLIFFCELLDRRLRATQEDGITHDFIRKQVWEEE
                     RDSLARFSADFLDKIEKVLIA"
     gene            1313..2212
                     /locus_tag="VIG33_11420"
     CDS             1313..2212
                     /locus_tag="VIG33_11420"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEY8271488.1"
                     /translation="MSFNPELWTEIKEFKFTDLTYHRAKDQGTVRIAFNRPEVRNAFR
                     PQTVDELYTALEHARVTSDVGVVIITGNGPSPKDGGWAFCSGGDQRIRGKDGYKYEDH
                     KGAASMNVKDAARLGRLHILEVQRLIRFMPKVVMAVVPGWAVGGGHSLHVVCDLTLAS
                     QEHAIFKQTDPDVASFDSGYGSAYLAKMVGQKRAREIFFLGRNYTAQEAFDMGMVNAV
                     VPHHELEKVALEWAREMNSKSPTAMRMLKYGFNMVDDGLVGQQLFAGEATRLAYGTEE
                     AAEGRDSFLEKRPKNFSKFPWHY"
     gene            2318..>2874
                     /locus_tag="VIG33_11425"
     CDS             2318..>2874
                     /locus_tag="VIG33_11425"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HEY8271489.1"
                     /translation="MPYSFHREKSKSGKPLGIPGYTFGSKEVAKSPLTLSDLELLKRT
                     VLWTDEDTRYIRMSRGLLEDQTDEILDVWYNFIKAYPHLSFYFSTPDPMQDRKLNKAY
                     MDEVKLRFKQWILDTAEANYDQQWLDYQYEIALRHHRTKKNKTDSASSSEIVNFRYIP
                     AFIYPITATLKPFLSKKHNFSAEDV"
BASE COUNT          800 a          561 c          658 g          855 t
ORIGIN      
        1 cattcaaagt attgagcccg agatctatct tgggagttgt ccgttgatga ccttgatatc
       61 ttttgaagaa aaggtaagtc taccggctaa gttgaaaatt ctgcagaagt tgcgcgattt
      121 tttccccaaa acccaggttg tggtcgcttt gcagggcgat gaaaaacctg aagattacga
      181 tttgttaaaa aacgcaggcg ctcactattt gatcctttcc gaggaaatta agaacagtgg
      241 taaattgtac tacttgtcag cggtgctcgt gcaaggagcc tattttccgg tgccggtaac
      301 ggatgtcttt cccagtaccc agttgagttt taatgcctat cataaattat tattaaatca
      361 aaaatttctt ccagtgattt tttcagggtt ccctattagc gataaaaaat accgaaagct
      421 tgaaccttcg aagcagattt atgttcgtcg tgaaagcctt gacgaatatc gtaagtatgt
      481 tgaagcctat catgaccgca cgggatcagc tttgaaaaaa cgttgccgtg tagtgatgat
      541 gaatttggtg ggaattcaat gcgaattgct gcttcaattg actttagatg tcgaagtgag
      601 gacagagatc tcagtaaaag aaaagatatc tcgcttcgtg caaagctctt tagaactcgg
      661 ggattatttg caagattgcc ctgatgtttg gaatgttatc gcccactcct tagactttaa
      721 attttgccat tatgaacgga gcatttatat tttagcctat gcgctttata tttctgctaa
      781 agcgaaattg acggtcttga gtgatttagt gatgacggtc cttctagctg atctaggtat
      841 tttagagttg cctgtggagt gttacaaaac tcttcaaagc agaggtgaaa agcagttgtc
      901 gcctcaagac cttgagtatt tccataatca ccccattgcc tccctcaatc gtttgaagtt
      961 tcatcaggtt gagctgtctg aggaagttca aagtgcactt gtttgtaccc atgagcgcaa
     1021 tgatttaaaa ggttttccta accaagtgcc tggagataaa attcctgtgg ccgcaaagtt
     1081 aatttttttc tgtgaactct tagatcgccg tctccgggcg acccaggaag acgggatcac
     1141 tcacgacttc attcgtaaac aggtatggga agaagaaagg gactccctcg cgcgttttag
     1201 cgctgatttt ttggacaaaa tagaaaaagt cctcattgcc taacaaaaga ggaccaggta
     1261 aatacagttc agaaaacgaa tatgttgtat ggatcattgt atgggagtgt ctatgtcttt
     1321 taatcctgag ctttggactg aaatcaaaga atttaaattt accgatctaa cctatcatcg
     1381 cgccaaagat cagggcacgg ttcgtattgc ctttaatcga cctgaggtcc gcaatgcttt
     1441 tcgccctcaa actgtggatg aactttatac cgctcttgag catgcacgtg tgacttcaga
     1501 tgtgggggtg gttatcatca caggcaatgg accttctcca aaagatgggg gttgggcttt
     1561 ttgttctgga ggcgatcaaa gaattcgtgg aaaagatgga tataaatacg aagatcacaa
     1621 aggcgcagcc tcaatgaatg ttaaagatgc agcccgttta ggaagattgc atattctcga
     1681 agttcagcgt ttgatccgct ttatgccaaa agtagtgatg gctgtagtcc caggttgggc
     1741 ggttggaggg ggtcactccc tgcacgtggt gtgcgacctc acccttgcca gtcaagaaca
     1801 tgcgattttt aaacaaactg atcctgacgt tgcgagtttt gacagtggtt atggttctgc
     1861 ttacttagcg aaaatggtgg gtcaaaaacg agcccgcgag atttttttct tagggcggaa
     1921 ctacaccgct caggaggctt ttgatatggg aatggtcaac gcagtcgttc ctcatcatga
     1981 gttggaaaaa gtggctcttg aatgggcgcg ggaaatgaac tcaaaaagtc ctacagcaat
     2041 gcgtatgttg aagtatggct tcaatatggt ggatgatggc ctggtagggc agcaactttt
     2101 tgccggtgag gccacacgtt tagcttatgg tacggaagaa gccgcagagg ggcgggactc
     2161 tttcttggaa aagcgtccaa aaaacttttc caagttccct tggcattatt agagcatttt
     2221 atttaatttt agtctgtaaa tcgtttaatc ttccaaactt ctcctctgat tcacgacatc
     2281 tcctttgtaa aatttcacaa tatgcaaagg agatgtcatg ccctattcct ttcatcgtga
     2341 gaaaagcaaa agtggtaaac ctctcggtat acctggttat acctttggat ctaaggaggt
     2401 ggctaaaagt cctctgactt tatcagatct cgagcttctc aagcgcactg ttttatggac
     2461 ggatgaagat acccgatata ttcgtatgtc acgagggctt ctagaagacc aaacggatga
     2521 gattttggat gtatggtaca atttcatcaa agcttaccct catctttcat tttattttag
     2581 tacacccgat cctatgcagg atcgtaaact caataaagct tatatggatg aagtgaaatt
     2641 gcgatttaag caatggatct tggatacggc cgaggcaaat tatgatcagc aatggttgga
     2701 ttaccaatat gaaatcgctc ttcgtcacca tcgaactaaa aaaaataaaa cggattcagc
     2761 ctcttccagt gagattgtta atttccgata tattccggcg tttatttatc cgattaccgc
     2821 gacattaaaa ccttttcttt ctaaaaagca caattttagt gcagaagatg tgga
//
LOCUS       DATBTE010000307         8861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1269733, whole genome shotgun sequence.
ACCESSION   DATBTE010000307 DATBTE010000000
VERSION     DATBTE010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 8861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8861
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269733"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VIG33_11430"
     CDS             <1..976
                     /locus_tag="VIG33_11430"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY8271490.1"
                     /translation="VIENSSSFFAGLHKADVILSVNGQKASPLSPQELSESLHGEEGS
                     MLKLTVLRHGEIKNITIVRTEQTLSTVSAGVLQGIKPVGVLTINKFAIESCIEVKNAL
                     KKLKQQGIQGLLLDLRDNPGGQMEEASCVTSLFVGPNKKIYQVKFLVDEVQDDVQEEA
                     SFGSEEQQYEGPMAVLINSGSASAAEIVAGALQDYKRAVLVGERSFGKGSFQEGEAWS
                     KNPHIAIFETKGFYYLPSGRTPQLVGLEPDVKVGLKDSFALREVDQFWYPLVPESLSI
                     QMETAVGLQTDIATCISFDSDFLSSDDPEIKEAHRVLNCREIAERGTR"
     gene            973..1653
                     /locus_tag="VIG33_11435"
     CDS             973..1653
                     /locus_tag="VIG33_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine esterase"
                     /protein_id="HEY8271491.1"
                     /translation="MISTDLFKFKFIPAKKRSEKLMIVLHGKGDSIRPFRHFDKEMKL
                     PEMNYLLLNAPKKFLDGYSWYGDPPFERVGVLKIRERLLQLLAELEMQGWKSESIFLF
                     GFSQGCLISADVGMNYPKRLGGIVGVSGYFHFYPRWRQRLSKAALKTPWIFTHGRQDD
                     LLKLDETKYGVDKLRAAGIGIDWIELDKDHDLTEKEMPMIRRWVRARLMDNKLVSQNF
                     RSKPTTIP"
     gene            complement(1617..2303)
                     /gene="lexA"
                     /locus_tag="VIG33_11440"
     CDS             complement(1617..2303)
                     /gene="lexA"
                     /locus_tag="VIG33_11440"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY8271492.1"
                     /translation="MIESSPLPLTIKEKAFLDFVEGFQKSFGISPSYQEIRDHFGFAS
                     FNSVQNYLKQLARKGYVRNQLNQKRAIQLVLPSLSISAGPSLTPLLQSKEEVLSLPLR
                     GKVAAGTPFESLKHDEFISVPATMVRQADKTFALRIQGESMINEGILEGDTILVQQQN
                     NAANGDLVVAMIENEATVKRFYLCTPPRGSHDELMVELRPANEKFQSMWFSPYEISIQ
                     GIVVGLLRKF"
     gene            complement(2380..2637)
                     /locus_tag="VIG33_11445"
     CDS             complement(2380..2637)
                     /locus_tag="VIG33_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271493.1"
                     /translation="MLERGGFSFVALLVINPIAILFFQNCSFLPTTQNALASREVNPQ
                     KRFMSSLATPKKIENTQLIKVASPTIADCKSSLENCPVSEE"
     gene            2815..3711
                     /locus_tag="VIG33_11450"
     CDS             2815..3711
                     /locus_tag="VIG33_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HEY8271494.1"
                     /translation="MRTVAVLFLSVILTSKALAADSTDLSKKCMESLKAFPGKVDLSL
                     LQKACDRVQLDDVCRSVNGTPIFHYEKIAKNTEAKKVLVFSLIHGDETHAGTVGRYWM
                     ERLESFDPRNSWRVVPVLNPDGVQAKTRTNAHNIDLNRNFPTKDWDQLAKTYWQKQTK
                     SNPRRNPGDSAGSEAETKCALKHLEEFKPDFIVSVHTPLKVLDFDGPKVNFPKYDYLP
                     WKSLGHFPGSLGRYMWFERRTPVLTTELREDLPATLAPLERLQDVIGTLVKIEIKAKR
                     ADAIIPNQVVKTSVSPEVNEKN"
     gene            complement(3708..4145)
                     /locus_tag="VIG33_11455"
     CDS             complement(3708..4145)
                     /locus_tag="VIG33_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271495.1"
                     /translation="MSDLLNPGLEQLKIALEENPEVCLEDSPEVKSYVYQQIAEFQPY
                     VTPQTVVAVILKDPTKLALQYEAEGKKFELNDLKSLYRISITLSEDRAKITAEGVDKD
                     IFVAIRTAKDLLLKKLAAIQDSVVTQQERNLEIHQALQNTMLH"
     gene            4361..5077
                     /gene="ung"
                     /locus_tag="VIG33_11460"
     CDS             4361..5077
                     /gene="ung"
                     /locus_tag="VIG33_11460"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY8271496.1"
                     /translation="MNTISELKKEAKVALPQLEKSWLTLLQPEFEKDYMKNLRSFLIS
                     QKNSKKIIYPKGEEYFAAMNLTPLDKVKVVIIGQDPYHGPGQAHGLSFSVRKGVRFPP
                     SLMNIFKELHDDLGLPLPKSGDLTHWAEQGVLLLNAVLTVEGGKAAAHQGKGWELFTD
                     QIIATLNKNREHIVYLLWGSYAQKKAAFVDRTKNLVLESPHPSPLSAYRGFFGSKPFS
                     KINKYLREHGIKEIDWSLPS"
     gene            5213..5653
                     /locus_tag="VIG33_11465"
     CDS             5213..5653
                     /locus_tag="VIG33_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271497.1"
                     /translation="MAIIKILGVLGLVGSVVYAMDTAADKKGHEVKEKALATAQAAAE
                     YTKEQKAEMQKSLEDKMGKISGEIADLKKDAAAASGKVKKELDQEIKTLEKKQKSLQK
                     DLARWKKSTGKAWEDMKNGMSQAMDNISESYQKAKKEFSETKSE"
     gene            5660..6727
                     /locus_tag="VIG33_11470"
     CDS             5660..6727
                     /locus_tag="VIG33_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271498.1"
                     /translation="MTKFRSQLQYVNQWPSPKRFGDEVLLIYDNVFETNTKAAQWIKR
                     FPVRYAVKSGESLKSLQKFPQHMNMILQKTHNFSSKKLKIIALGGGSVGDFGGFVASI
                     YKRGVELIHIPSTWLSAMDSAHGGKTALNARGAKNQIGTFYPAKEVYLVKPLLLAQPS
                     ERNFEALGELIKMALISGGSLYKKLNQVSSLNSGTLWKLLPLVVTEKYKIVQKDPYEL
                     KGLRHVLNFGHTMGHVFEAHYELPHGVAVLAGLQFALEWSYERGFMNQAEYLKLMRAR
                     FWKKAVQAKQFSVWKNLNMMALLSDRPQKFLHYLEQDKKKTNMRNLRFVFLKSPGHPL
                     IAEISLKDVLPEIARQQSFWG"
     gene            6729..7919
                     /locus_tag="VIG33_11475"
     CDS             6729..7919
                     /locus_tag="VIG33_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEY8271499.1"
                     /translation="MKTFRFQGRIPASKSLMNRSLIVQSFEPALKLIGDSSCDDVTHL
                     KLSLKKLSCNKPGQRTVFDCGEGGTTLRFLALRVSREKGEFLLKGTPRLLRRPQKEVK
                     TLLKQLGAKVVFEKKGIRIISEGWKQPAEGIVLSAKDSSQFLSALFLNAWNLAFDLKI
                     TVRGKITSESYFLMTLKVLKDLGLKFSRRGNIFYIPRNQKVKLAQYTVESDLSSLFSV
                     ASFAGVSGKAIFKDFPKKSFQPDAAFTQVFKKMGIRNIQQGKILSVSSPKILKAVKVN
                     LQNAPDLFPVLAVLCSFAEGRSVLFGAPQLAYKESNRIQKTAELLKKAGISCKPRKDG
                     LEIQGRSGIIPVASFMFDPDKDHRLAMAAALLKYFGQEIKIKDPEVVCKSFPEFWKYV
                     GVQA"
     gene            7916..>8861
                     /locus_tag="VIG33_11480"
     CDS             7916..>8861
                     /locus_tag="VIG33_11480"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEY8271500.1"
                     /translation="MITVIIGQRGVGKTHLLKRIHRYTSDEKVPCFDLDQEIEKRAKK
                     TIREIFEREGEEVFRDLEKRIFQEIILSHPTCWLAVGGGFPVGIIPETVRILWVQRST
                     DHAGRIFLDRPRLDKDLSPLEEFEKRAKSREFHFKNSYDQVYLMPEGLIEVDPLEEDI
                     LIRHKKVFAGALTLLPELFLKKARLHGFFENYANRGIEFFEIRDDVLSHDQVQICLGS
                     LFSEKFIFSFRSGKTEEPPHSNQIEYYDWAIELGPIPEPFRFLKQRLIVSLHELREGE
                     SLEDAFHRLNEESGKCIHLKFAPIVNSFEDLLLGYFWQQ"
BASE COUNT         2561 a         1680 c         2108 g         2512 t
ORIGIN      
        1 agtcattgag aactcttcct cgttttttgc cggtctccat aaagcggatg ttattttatc
       61 tgtgaatggg caaaaggcga gtcctctttc cccgcaagaa ttgagtgagt cattgcatgg
      121 cgaagagggg agtatgctca aattgaccgt gcttcggcat ggggagatca aaaatatcac
      181 gattgttcgt acggaacaaa ccttgtcgac ggtaagtgca ggtgtgctac agggaattaa
      241 gccagtgggt gttttgacaa tcaataaatt tgccatcgaa tcttgcattg aagttaaaaa
      301 cgcccttaaa aaattaaaac agcaagggat tcaaggtttg cttctggatc tacgggataa
      361 tccaggagga caaatggaag aggcctcctg tgtaaccagc ttatttgttg ggccaaacaa
      421 aaaaatttat caggttaagt ttttggtgga tgaggttcaa gatgacgttc aagaagaagc
      481 ctcttttggt tccgaagagc aacagtatga agggcccatg gccgttttga tcaattctgg
      541 ctcggcgagc gccgccgaaa tcgttgcggg ggcattgcag gattataagc gggcagtttt
      601 ggtcggcgag agaagttttg gcaaaggctc tttccaagag ggtgaagcgt ggtcaaaaaa
      661 tcctcatatt gcaatcttcg aaacaaaagg tttttattat ttaccgtcag gaagaactcc
      721 tcagcttgtg ggcttagaac ccgatgtgaa ggttggcttg aaggacagct ttgcactgcg
      781 cgaagtggat cagttctggt atcccttggt tccagaaagt ctaagtattc aaatggaaac
      841 ggctgtgggc ctgcagacgg atattgccac ctgcattagc ttcgacagcg attttttatc
      901 ttccgatgat cctgaaataa aggaagctca tcgggttctg aattgccggg aaattgcaga
      961 gcgaggtact cgatgatctc gacggatctt tttaagttca aattcattcc agcaaaaaag
     1021 cgctcagaaa aattgatgat cgtcttgcac ggcaagggag atagtattcg cccctttaga
     1081 catttcgata aggagatgaa gctgccagag atgaattatc tgttgttgaa tgcgcccaag
     1141 aaattcttgg atggttattc ttggtacgga gatcctcctt ttgagagggt gggcgttctc
     1201 aaaatccgcg aaaggctttt acaactttta gcggagttgg aaatgcaggg atggaaaagt
     1261 gaaagcattt ttcttttcgg attctcgcag ggctgcctga ttagtgccga tgttggtatg
     1321 aactatccaa aacgcctggg gggtattgtg ggcgtgagtg ggtattttca cttttaccca
     1381 agatggcgcc agcgactttc caaggccgcc ttgaaaactc cgtggatttt cacccacggt
     1441 cgtcaggatg atcttttaaa acttgatgaa actaagtacg gagtggataa actgcgtgcc
     1501 gccggtattg gcattgactg gattgaactt gataaagatc acgatttgac ggagaaagaa
     1561 atgcccatga ttcgtagatg ggttcgcgct cgactgatgg acaataaatt agtgagtcag
     1621 aattttcgca gtaagcccac cacgataccc tgaatgctta tttcataagg cgagaaccac
     1681 attgattgaa atttttcatt tgctggacgc agttcgacca taagctcgtc atggcttcct
     1741 cgggggggag tgcagagata aaagcgtttc actgtggcct cgttctcgat catcgccacc
     1801 acgagatccc cattagcagc attgttttgt tgctgcacaa gaatcgtatc tccttctaaa
     1861 attccctcgt taatcataga ttcgccttgt atgcgcaggg cgaaagtctt gtcagcttga
     1921 cgaaccatag ttgcgggtac gctgatgaat tcatcatgct tgagggattc aaaaggagtc
     1981 cctgcagcga ctttaccccg aaggggaaga gacagaacct cctccttgct ttggaggagc
     2041 ggagttagag aaggtcctgc tgatatgctt aatgaaggca gtacaagctg gatggcgcgt
     2101 ttctgattga gctggtttct tacgtagccc ttacgggcga gctgcttaag ataattttgg
     2161 accgagttaa acgatgcaaa tccgaaatga tctcgaattt cctgatagga cggggaaatt
     2221 ccaaaactct tttgaaagcc ttctacaaaa tctaagaagg ctttttcttt tatagtaagg
     2281 gggaggggcg aactttcaat catacttaca tgtcaattac atatgtaaat aatatgtaag
     2341 tcaaaaatta attttgatgg tccaggttgg ttttttcacc tattcttcag agacggggca
     2401 gttctcaagg ctgcttttgc agtcagcaat agtcggcgat gcgaccttta tcagttgagt
     2461 gttttcgatt ttttttggag tcgctaggga actcatgaat cgtttttgtg gattcacttc
     2521 tcgtgaggca agcgcgtttt gggtggtggg taagaaagaa caattttgga agaataaaat
     2581 agctatcgga ttaatgacca atagtgccac gaaactaaat ccaccacgct cgagcatgca
     2641 gtcctcctca atgaatcctt gttactttat tctacggatg aagtggccca ggaccttagt
     2701 ctaaagttag aagttctcag ggtgagacaa agaaaagttt tgccaagaga aatcttgcct
     2761 tgtgagtgtg taaaggactg gcaataatta caaaagagac ataaaaagga ttttatgaga
     2821 actgtggccg tcctattttt gagcgtgatt ctaacttcca aagctttggc ggcggattca
     2881 actgatcttt ccaaaaaatg catggaatcc ttaaaagcat ttcctggcaa agttgattta
     2941 agcttattgc aaaaggcctg tgacagggtc caactggacg atgtttgcag gagtgttaac
     3001 ggaactccta tttttcacta cgaaaagatc gccaaaaata ctgaggccaa gaaggttttg
     3061 gtatttagcc ttatacatgg tgatgagacc catgcgggca ctgtgggacg ttattggatg
     3121 gaacgccttg agagttttga ccctcgcaat tcttggcgtg tggtgcctgt tctgaatcca
     3181 gacggtgttc aggccaagac gagaaccaat gcccataata tcgatttgaa tcgtaatttt
     3241 cccactaaag attgggatca acttgccaag acttattggc aaaagcaaac caagtcaaat
     3301 ccccgccgca atcctggcga tagcgcggga agtgaagcag aaaccaaatg tgcgttgaaa
     3361 catctggaag agtttaaacc ggattttatc gtctccgtgc acaccccact caaagtttta
     3421 gacttcgacg gacccaaagt gaatttccca aagtatgatt atctgccttg gaaatcctta
     3481 gggcattttc caggtagcct aggccgttac atgtggtttg aacgccggac cccagtttta
     3541 accactgaac ttagagaaga tctgcctgcc accctcgctc ctctggaaag acttcaggat
     3601 gtaatcggga ctctggtcaa aatcgaaatc aaagcaaaac gagcagacgc gatcattccc
     3661 aatcaagtgg tgaaaacttc ggtctctcct gaagtgaacg aaaaaaacta atgaagcatt
     3721 gtgttctgga gagcttgatg aatctctaaa ttgcgttctt gttgtgtcac aacgctatct
     3781 tgaatcgctg cgagtttttt cagtaaaaga tcttttgctg tgcgaatggc cacgaaaata
     3841 tctttgtcga cgccttcggc tgtaatctta gctcggtctt cacttaatgt gattgaaatc
     3901 ctataaaggg atttcaagtc attaagctcg aatttttttc cttccgcttc atattgaagc
     3961 gctagtttag tggggtcttt caaaataaca gcgaccacgg tttgtggagt tacatagggc
     4021 tgaaactctg caatttgctg gtaaacatac gattttactt cgggactatc ttcaaggcaa
     4081 acttcaggat tctcttcaag ggctatttta agttgctcta gcccggggtt cagaaggtca
     4141 gacataaggg cctcctattc cgttactaca ggtttcttct atcataacaa tgagttccaa
     4201 ttaagtcagg tcaaggaacc gcgaaataaa aaatatattt aaaatggcat atatttgatt
     4261 taaaatctcg accttggacg gtccagggag tctttgagac tcctttcgcc ttaaaatgag
     4321 atgatcaaga aatcgtttaa aagcctatgt aggagctatt ttgaacacta tttcggaatt
     4381 aaaaaaagag gcaaaagtgg cgctgccaca acttgaaaaa tcctggctga cactgcttca
     4441 gcctgaattc gaaaaagatt atatgaaaaa tttgcggagt tttctcattt cgcaaaaaaa
     4501 ctctaaaaaa atcatctatc cgaaaggtga agagtatttt gcagcgatga atttgactcc
     4561 ccttgataag gtaaaagtgg tgattatagg ccaagatcct tatcatggtc ctggacaagc
     4621 tcatggcttg agtttttcgg ttcgcaaggg agtgcgtttt ccgccctcct tgatgaatat
     4681 tttcaaagaa cttcatgacg acttgggttt gccgcttcca aaaagcggag atcttaccca
     4741 ttgggcggag caaggtgtgt tgctgctgaa tgcagttctt acggtggaag gtggtaaggc
     4801 ggcggctcat caaggcaaag gatgggagct gtttacggat caaatcattg cgactttgaa
     4861 taaaaatcgg gaacatatcg tgtacctttt atggggcagc tatgcgcaga aaaaagctgc
     4921 atttgtagat cgcacaaaaa acctagtgtt agaatccccc catccctcac ccttgtctgc
     4981 ttatcgaggc ttctttggtt caaagccttt ttcgaaaatt aataaatatt tgcgtgagca
     5041 tgggattaaa gaaatagatt ggtctttgcc ctcttaaaag cttttcttca acgtttaaaa
     5101 agtcgaggct tttatactga ttctaggcga acttatagga agtacaaaaa aatatggatt
     5161 tcaaattgaa actcgcggaa aataattcga aaccaagcaa ggagcacagt ttatggcaat
     5221 cattaaaatc ttaggtgtgt tgggtctagt gggatccgtc gtttacgcca tggatacagc
     5281 cgctgataaa aaaggccacg aggttaaaga aaaagcctta gcgacggcac aggctgctgc
     5341 ggaatatacc aaggaacaaa aagcggaaat gcaaaaaagt cttgaagata aaatgggaaa
     5401 aatatctggc gagattgcag atttgaaaaa agatgcggca gcagcttcgg gcaaagtaaa
     5461 aaaagaattg gaccaggaaa tcaagacgct ggagaagaag caaaaatcct tacaaaaaga
     5521 tttagctcgt tggaaaaaaa gtacgggtaa agcctgggaa gatatgaaaa acggaatgag
     5581 tcaggctatg gataatattt ctgagtctta ccaaaaagca aaaaaagagt tctcagaaac
     5641 taagtctgag taagtcggag tgacaaagtt tcgatcccaa cttcaatatg tgaatcaatg
     5701 gccttctcca aagagatttg gtgatgaagt tttattaatc tatgacaatg ttttcgaaac
     5761 gaatacaaaa gcagcccagt ggataaagcg ctttcctgtg cgctatgcgg tgaagtccgg
     5821 agaatcactg aagagccttc aaaaattccc gcaacacatg aatatgattt tgcaaaagac
     5881 ccataatttc tcctctaaga aattaaaaat aatcgcccta ggcgggggca gtgtgggaga
     5941 ctttgggggc tttgtagcga gtatttataa gcgtggtgtt gagctgattc atattccaag
     6001 cacctggctt tcagctatgg attcagccca tggtggaaag accgctctga atgcccgggg
     6061 tgctaaaaat caaatcggca cattttatcc tgcgaaagaa gtttatcttg tgaagccctt
     6121 gttgttggcg cagccttcag aacgtaattt cgaggcgttg ggtgaactta ttaagatggc
     6181 gttgatttcc ggagggagtc tttataaaaa actgaatcag gtgagttcgt tgaattcggg
     6241 aactctttgg aaattgcttc ctttggtggt gacagaaaaa tacaaaatag tccaaaagga
     6301 tccttatgag ctcaaaggac ttcgccatgt tctcaacttt ggacatacaa tgggccatgt
     6361 ctttgaagct cattatgagt taccccatgg agtagcggtt cttgccggct tgcaattcgc
     6421 ccttgagtgg agttatgagc ggggctttat gaatcaggcc gagtatttaa aattaatgcg
     6481 agcccggttc tggaaaaagg cggttcaagc aaagcagttc agtgtttgga aaaatcttaa
     6541 tatgatggcg ttattaagcg ataggccgca aaaatttttg cactacctgg agcaggataa
     6601 gaaaaagact aatatgcgga atttgcgttt cgttttttta aaaagtccgg gtcatccact
     6661 catcgctgag atctcattaa aagacgttct cccagagatt gctcgtcagc agtctttttg
     6721 ggggtaagat gaaaactttt cgttttcaag gccgcattcc tgcttccaag tctcttatga
     6781 atcgctcctt gatcgtacaa agttttgagc cagcattaaa actgattgga gactcaagct
     6841 gtgatgatgt cactcacttg aagcttagcc ttaaaaaact cagttgtaat aagcccggcc
     6901 agaggaccgt gtttgattgc ggcgaaggcg ggaccacctt gcgttttctt gcgctcaggg
     6961 tttcgaggga aaagggggag tttcttctta aagggacgcc acgccttctc cgtcgaccgc
     7021 aaaaagaagt aaagactctt cttaaacaat taggggctaa ggttgttttt gaaaaaaaag
     7081 gaattcgtat tatttctgaa ggttggaaac agccagcaga aggaattgtc cttagcgcta
     7141 aggattccag tcagtttttg tcagcgttgt ttttgaacgc ctggaatttg gcatttgatc
     7201 ttaaaataac ggttcgtgga aaaatcactt cggaatccta ctttttaatg actttaaaag
     7261 tattaaaaga cttgggcctt aagttctctc gcagaggaaa tattttttac attcctcgta
     7321 atcaaaaggt caaactggct cagtacacag tggagtctga tctaagttct ttatttagcg
     7381 tggcttcttt cgcaggggtc tctggcaagg cgatttttaa agattttccg aagaagagct
     7441 ttcagccaga tgccgcattc actcaggttt ttaaaaaaat gggaattcgt aatattcaac
     7501 aagggaaaat cctttccgta agttcaccga aaattttgaa ggcggtaaaa gtgaacttac
     7561 aaaatgctcc agatttattc ccggtccttg cggtgctttg cagttttgcg gaaggacgat
     7621 ctgtgctttt tggagctccg cagttggcct ataaagaatc caatcgtatt cagaagaccg
     7681 cggagctttt gaaaaaagcc gggatttctt gtaagccgcg gaaggatgga ctggaaatcc
     7741 aaggccggag cggaattatt ccggtggctt cttttatgtt tgatcctgac aaagatcatc
     7801 gcttggcaat ggctgccgcg cttttaaaat atttcggaca agaaattaaa atcaaagatc
     7861 cagaagttgt ttgtaaaagt tttccagagt tttggaagta tgtgggagtt caggcatgat
     7921 tacagtgatt attggacaaa gaggtgtggg taagacccac ttattaaaaa gaatccatcg
     7981 ttataccagt gatgaaaaag tgccttgttt tgaccttgat caagagatcg aaaaacgtgc
     8041 aaaaaaaact attcgtgaaa tttttgaacg agaaggggaa gaggtctttc gagatctgga
     8101 aaaaagaatt tttcaagaaa taattctgtc tcatcctact tgttggttgg cagtgggcgg
     8161 cggctttccc gtggggatta ttcctgaaac agtgcgaatt ctttgggtcc agagatctac
     8221 agatcacgca ggaagaattt ttttggatcg tccccgcttg gacaaagact tgtcaccact
     8281 ggaggaattc gaaaaaagag cgaaatcacg ggaatttcat tttaaaaatt cttacgatca
     8341 agtttattta atgcctgagg gactcatcga agtagatcct ttagaagaag atattttgat
     8401 tcgccacaaa aaagtctttg ctggagcttt gactctgtta ccggaattat tcttaaaaaa
     8461 agcccgtttg catggatttt ttgaaaatta tgccaatcgc ggcatcgagt tctttgaaat
     8521 tagggatgat gttttaagtc acgatcaagt tcaaatatgc ttaggctctt tattttcgga
     8581 gaaatttatt ttttcctttc gctcgggaaa gacggaagag ccgcctcata gtaatcaaat
     8641 tgaatactat gactgggcca ttgaattggg acccatacct gaaccttttc gttttttaaa
     8701 acaacgcttg attgtttctt tgcatgagct tcgagaaggg gaatcgttgg aagatgcttt
     8761 tcatcgatta aatgaggaat ctgggaagtg tattcatctg aagttcgctc ccatagtaaa
     8821 ttcgtttgaa gatttactat taggttattt ctggcagcaa g
//
LOCUS       DATBTE010000308         2581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_860507, whole genome shotgun sequence.
ACCESSION   DATBTE010000308 DATBTE010000000
VERSION     DATBTE010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860507"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VIG33_11485"
     CDS             complement(<1..794)
                     /locus_tag="VIG33_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY8271501.1"
                     /translation="MIDIKLLEKKAENGVSYFDEYKQSLVNRGASTEVLEQIIELNKK
                     RKEMITSAESEKAKQNKLSGEIGKLKREGGDTSALMAEVEKLKGLVKDLELKATEIDQ
                     QVTHVALTLPNKLHSSVPVGHSEKENLQVKLVGTPPKFSFKSKEHWEIGEALDIIDFE
                     RAAKTTGTRFAFLKGAAAQLERCLIQFMMDLHSQKHGYTEMIPPFMVNSDSLLGTGQF
                     PKFKEDVFNLTGTDLYLIPTAEVPVTNYYNNEILNETDLPKSFCAYS"
     gene            complement(814..1122)
                     /locus_tag="VIG33_11490"
     CDS             complement(814..1122)
                     /locus_tag="VIG33_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271502.1"
                     /translation="MNISDKMKGLSTNVQDGVKNSSVLLFCLSLRILTGFMLGLTLGL
                     IVQELVGYGTLALIFMMVVVMGLVVRVTSGWNIGKILIFDLILILVAMLLRMYILVAP
                     "
     gene            complement(1129..>2581)
                     /locus_tag="VIG33_11495"
     CDS             complement(1129..>2581)
                     /locus_tag="VIG33_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8271503.1"
                     /translation="SDKIKEKSTNSEMMFIGDQYARTGDCLAAQAEFKAAFEIDPKSG
                     MAAVKAAKCLWQLNQSFESIEWLTKAIKAEPNLITAYVLQADYLSQRYNFAGAANALT
                     NAARISPNNYEVFRGMALIEFRRNNMQGTINFAQRALRVYDADIETYTLLAKANAALY
                     GSTVSSTRKNTEAREKYQHEALRYATKAVEIDATNAEAQITYAKVMAQINGVDSGVNY
                     VKELIRKYSYSLEYRVALAEIFKSEDRYTQAQEYYEQVTEADSKNKKAFIGLGECYKA
                     VGLVDRSLKAFLTAAVLDPSDGEALFQAGKLYMESGRFEEAIQQFKRVQSLNSHFPRV
                     YYFIGKSAFLAGDFNLALEAVKKEKQENPNLADSYMLAGEIYGAKKQYAECAAEYSQA
                     MKLRAQGADAYVKAAQCYRQSGAIDIAQDLLSMASSRESGYADIYKEQGAIYEVRGDI
                     RSAVQSYNKYLGLSPNALDRGEIESRINRIGGR"
BASE COUNT          711 a          639 c          491 g          740 t
ORIGIN      
        1 gaataagcac agaaactctt cggcaaatcc gtctcgttca gaatttcatt attataataa
       61 ttcgttaccg gaacctctgc ggttggaatc aaataaagat cagtccccgt aagattaaat
      121 acgtcttcct taaacttggg aaactgtcct gttcccaaga gactatcgct attcaccata
      181 aatggcggaa tcatctccgt atatccgtgc ttctgagagt gaagatccat cataaattgg
      241 atcaagcagc gctcaagctg agccgccgcg ccttttaaaa aagcaaagcg ggttcctgtg
      301 gttttcgcag ctcgctcaaa atcgatgata tccagcgctt caccaatttc ccaatgttct
      361 ttacttttga aagaaaattt cggaggtgtg cccaccaact tcacttgcag attctctttt
      421 tcagaatgtc caacaggaac actgctatga agcttgtttg gaagagttaa ggcaacatga
      481 gtgacctgct ggtcaatctc tgtggccttc agctcaaggt ctttcactaa gcctttcagt
      541 ttctcaactt ccgccatgag tgctgaggtg tctccgcctt cgcgcttgag cttgccaatt
      601 tctccactta gcttgttttg cttagctttt tcgctttcgg ccgaagtaat catctcttta
      661 cgttttttat taagctcaat aatttgctca agaacttctg tggaagcccc gcggttcaca
      721 agactttgtt tgtattcatc aaagtaagaa acaccattct cggccttctt ttcgagaagc
      781 ttaatatcga tcattgctaa ggaccctttc tagttacgga gcgacgagaa tatacatcct
      841 gaggagcatt gccacaagga tgagaattaa atcaaaaatg agaatttttc caatgttcca
      901 gccggaggtg actctcacca cgagacccat caccacgacc atcataaaaa tcagggccaa
      961 tgttccataa cccacgagtt cctggacgat aagcccaaga gtgaggccga gcatgaaccc
     1021 cgtcaaaata cgaaggctca aacaaaataa cagcaccgag gagtttttta ctccgtcttg
     1081 aacgtttgtc gataggccct tcattttatc tgaaatattc attttaccct acctgccgcc
     1141 aatacggtta atacgagatt caatttcacc acgatctaaa gcgtttggcg atagtcctaa
     1201 atatttatta taagactgca ccgctgagcg aatatctcca cggacttcat aaatcgctcc
     1261 ctgctcttta taaatgtcag cataacccga ctcacgagaa gacgccatag atagcaaatc
     1321 ttgcgcgata tcgatagcgc ccgactgtcg ataacactga gcggctttga cataggcatc
     1381 cgccccctgt gcccgtaact tcatggcctg agaatactcc gccgcacatt ccgcatattg
     1441 cttttttgcc ccgtagattt ccccagctaa catatagctg tcggctaaat tcggattctc
     1501 ttgtttctct ttttttacag cttccagcgc taaattgaaa tcaccggcta aaaacgcaga
     1561 tttaccaata aagtaataga cccgcggaaa gtgagaatta agactttgaa ctcgcttaaa
     1621 ttgctggatc gcctcttcaa aacggccact ctccatatag agtttgccgg cttgaaataa
     1681 agcctcgcca tcggaaggat ctaaaaccgc cgcagttaaa aaagctttta aagaacgatc
     1741 cactaaaccc acagccttgt agcactcacc taagcctata aaagccttct tattttttga
     1801 atctgcctct gtcacttgct cataatattc ttgagcttgt gtataacggt cctcagattt
     1861 gaaaatctct gccagtgcga ctcgatactc taaagaatag gagtatttcc taatgagttc
     1921 tttcacatag ttcacaccag aatccacacc attaatttgc gccatgacct tagcataagt
     1981 aatctgagct tcagcattag tggcatcgat ttccacagct ttggtggcat agcgaagggc
     2041 ttcgtgctga tacttttcgc gagcctctgt attttttcgt gtcgaagata cagtactgcc
     2101 ataaagagct gcattggcct tagctaaaag cgtataggtt tcaatatctg catcataaac
     2161 acgaagtgcg cgctgggcaa agttgatcgt accttgcata ttatttctac ggaattcaat
     2221 caaagccatt ccacgaaaca cttcatagtt attcggagaa attctagccg catttgttaa
     2281 ggcatttgct gcaccagcaa aattataacg ctgggacaga taatccgctt gaaggacgta
     2341 ggcagtgatc aaattgggct ctgccttaat tgcttttgtc agccactcaa tggattcaaa
     2401 actttgattc aactgccaca aacactttgc tgctttgacc gccgccatgc cgcttttagg
     2461 atctatttca aaggcggcct taaactcggc ttgcgccgcc aaacagtctc cagtgcgtgc
     2521 atattgatcg ccaataaaca tcatctcact gttggtagac ttttccttaa ttttatccga
     2581 g
//
LOCUS       DATBTE010000309        15552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1611901, whole genome shotgun sequence.
ACCESSION   DATBTE010000309 DATBTE010000000
VERSION     DATBTE010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 15552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15552
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611901"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VIG33_11500"
     CDS             complement(<1..443)
                     /locus_tag="VIG33_11500"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HEY8271504.1"
                     /translation="MKKYYISNGQENFGPLTEQEIMEAVRKGQINLFDMIFHDQLSEW
                     VMLIQHPDFSDQGSEEINASEEKSHIVLGLLSVENSQENTNNAFILDEDTPELTAVCW
                     HIKNDPDKELTFLDVLFLLSTKKISEHSLISKNRVGPWLPLAKWE"
     gene            complement(478..1119)
                     /locus_tag="VIG33_11505"
     CDS             complement(478..1119)
                     /locus_tag="VIG33_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271505.1"
                     /translation="MKKITILLCLAFILISACMLYLVKVGVSLRSAPLIAASVISPDR
                     QNIAEGVVFRLSPEFQNAHYVLWGFLPLSPESQKIFELLKAEYEKIFRQPVHVLANAE
                     TASAEEIRTCEKPCWLLLPMDKANELTPNELINLKIRPLGENYFNVTWVPFAKREQVS
                     SHCMAEKRLTLECLRTLAIEESQKKMKPGAQYFFMKKYNEKDFFLFFQEFSQL"
     gene            complement(1173..1859)
                     /locus_tag="VIG33_11510"
     CDS             complement(1173..1859)
                     /locus_tag="VIG33_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8271506.1"
                     /translation="MAHILIVDDQQSVLLTLEALLKSEHHYVTACTNAIDAMEHLKQE
                     PFDLVISDVVMSVGGTGLALLRTIRNQPQLARLPVILLTGKREKADVEKGIQAGADDY
                     VVKPIDPSLLLEKVKNIVDKAQQGPSRFVEAAVKVKAEFETKTEIISLSELGCTLQSN
                     ISATAGALIKVHSEVFKAIGIPMPTLRIVSTEELPSQDGQHKIVGNFVGWSERELTPL
                     RLWVRSRTRV"
     gene            complement(1873..4383)
                     /locus_tag="VIG33_11515"
     CDS             complement(1873..4383)
                     /locus_tag="VIG33_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8271507.1"
                     /translation="MDKSSLFHFLLRRFGTQFGVLLLFASSFYYFQDNGSKSDIYTLS
                     LLFEVRQGIYQASRNSAQYLVNGKGKDLDDYNREIVQLGQRFSELADGFKKVPGQYKK
                     FVEIQKKSRAYFLSLNDKIERKQSGTIKGDRAVAATSESSELISLLDGMIKDISENSS
                     DPWNFFGMTQPKALWYFLMGIAISCCLILMSHYLREKAYEDQKKDISELKIQSILLDG
                     ILNSISEALIVVDEKGQFTRYNTAAQRIIGNKIKSISSEEDAKAFGFFDINSLQQIGL
                     QELPFARALQGEQVEDIEILVHNEMNPEGIYISISSRYLRDIDGGIHGALVVFRDISR
                     RKATEQEWLRARESALETARMKSDFLAAMSHEIRTPMNGVIGMSTLLAETKLTEEQED
                     YVGIIKRSAEALLRLINDILDHSKIEAGKIQINPRPFDLKFLCEDVLELFFPAVREKN
                     IRLEISFLGREEWAFVADPERLRQVLVNLIGNAVKFTERGYIQIAVESMKSYANKATL
                     KISVMDTGAGMTEEESQSLFQKYFQTKTGMKYGGTGLGLSICRQLIELMGGQIGLHSK
                     LGLGTTFWFTLDLPETTLGQISIQHESHFAPVFNGSVLLAEDQLVNQRVAVTYLQKLG
                     LEVDVANNGQIALEKALSNSYDLIFMDCQMPVMTGYEATEKIRERQKGEKTPIVALTA
                     EGASGERNICFSVGMDDFLTKPLELDRLTEVLHRWLKSSPKVLDEAALEKLRKFMVNN
                     KSLTEALIEDFMNTAPDLVRTIASGCHENNLEGVQESAHALKSTSATLGATGLAKLCQ
                     TIEDAASVEEVKLLLVTVEDQLTKSLRELQKYATRAAA"
     gene            complement(4478..5821)
                     /locus_tag="VIG33_11520"
     CDS             complement(4478..5821)
                     /locus_tag="VIG33_11520"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8271508.1"
                     /translation="MQSQVSQANVLGMDSQTFNPTTNGLDFVTVHSSATLDPGIVNIG
                     FFLNYAVNSLPYVYGINGSGQARTKFNDRLFSSDLNFGVGLGDNWDMGFALPYLWSQS
                     VDDGGQLGKYSQTGMTGQRWNSKYRFFNGDRWGWAAVASVDQSLVENNPYTGENPGPT
                     YNLELVADVMLHNVLLGFNIGHRWRNPGASLAATYGITPLPNQWIYSVAASYLLASLD
                     TKLIWELYGSYPDGKSNDSQTLNISDRERSSLETLVGMKYDWTTNLAVHFGGGTEIYH
                     GTGTPAWRIYSGINYAFGPVWEKRSEVQPRRDLTKIPKVQNFTLTFLRFKFDSTELDP
                     SSYKNLDIVVRMIRETPGVQSITIEGHTDSVGSESYNQKLSQSRANVVKTYLKEHMLE
                     LAKVDFQGIGYGESRPIANNGNYQGRAKNRRVVIRVVRKIMIGDKPETEESSITF"
     gene            complement(5920..7278)
                     /locus_tag="VIG33_11525"
     CDS             complement(5920..7278)
                     /locus_tag="VIG33_11525"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEY8271509.1"
                     /translation="MIPVILSGGSGTRLWPVSRANFPKQFCDLFEDSLQSLTLKRCQS
                     LGPLWIITGESLKTLTESNLKELHLSAVTTIYEPVAKNTAPAVALLAHLLVLRGLGNE
                     VVGVFPSDHLIQDTEAFYQAVREAEQLALQNKIVTLGIPPDHPATGYGYIQKDSQSNK
                     VLKFHEKPSLEKAEKFLASGDFFWNAGMFIFKAKIMLEKFQNLQPDIFEIVSQLKEDF
                     SNLNEIYSQFKNISLDYAIMESLSSEELACVPADMKWSDVGSWDAVAQVAEGQASSKS
                     LEVNGHHNFVFGRQGKVYSFVGTDDLILVDTADALMVVKKGESQEVKQVVEALQKQGS
                     MLTKDHVFEKRPWGAFEILRDTAHFKSKVITVNPMSQLSYQSHSQRAEHWIITRGQGE
                     VVLDDQVILVRAGSHVFIPIQAKHRIRNTSDSILEFVEVQLGNYFGEDDIVRYQDDYK
                     RT"
     gene            7516..8625
                     /locus_tag="VIG33_11530"
     CDS             7516..8625
                     /locus_tag="VIG33_11530"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY8271510.1"
                     /translation="MSIPFIDLKTQYKALKGSIDSQIQKVLDHGAYVNGPEVAELEKK
                     LAQHTGAKYALTCASGTDALVLPLMALGVGPGDEVITTAFSFIATAETICLVGAKPIY
                     VDIDPLTYNIDLKAIEKAITPKTKAIMPVSLYGQMADMDEINAIAKKHNLFVIEDAAQ
                     SYGASYKDRKSCNASLVGATSFFPAKPLGCYGDGGAIFTNDENLLKTMKEIREHGSES
                     RYYHTRLGINGRLDTLQCAILLAKMERYDWEVEQRQKIADRYNEAFSAIKTPGFVVPL
                     VKNDRKSVWAQYTLSVENRNDFQKKLTDTGVPTSVHYPRTMPDQPWYKENTPCNSNIS
                     KARHAAEHVISLPMFPDMDTATQNKIIGAVVSAMK"
     gene            8635..9486
                     /gene="kdsA"
                     /locus_tag="VIG33_11535"
     CDS             8635..9486
                     /gene="kdsA"
                     /locus_tag="VIG33_11535"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEY8271511.1"
                     /translation="MSTGFKALEKTVTLQTSQGSITWGDGRNFVLFAGPDIIEDEGMV
                     LETGKEIQRVTKALGIPWILKCSFDKANRQSASSFRGPGSNNALKSLDKIKNELSCPL
                     LTDVHETFQVEDTAKVADVLQIPAFLSRQTDLLVAAAKTQKVIHIKKGQFLAPWDMKA
                     IANKAVAAGNSKLLLCERGTTFGYNRLINDMTGLIEMRKLGFPVIMDATHSTQLPGAT
                     GESSGGRGEMVWPLARAAMAVGVDGVFLETHPNPEVALCDGPTSLPLKNLEAMLKNLQ
                     KIWQTHF"
     gene            9505..10497
                     /locus_tag="VIG33_11540"
     CDS             9505..10497
                     /locus_tag="VIG33_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEY8271512.1"
                     /translation="MQKKVKNILFQILKIVFSVGLIFWLVQSGKLNFKALTNLLHLKY
                     VLPGFLLVAGSVYLTSERWRILLQTQSHHLPSWKTFKLTMIGVFFNFAVPGGVGGDIV
                     KAFYFTKDYPESKMAAATSVLVDRVLGLYGLILMAFLAMLFDWKHVQADSSLHFLWIA
                     IIALLFTASTAFALIFSQKIYDRGSFRRALEKLPFHEKTVRIYESVHLYGRNYKTLIG
                     SIFISLLSQSFSILFMILAGHASGLGTEMPWSTYFLVAPLGFMATAIPISPAGVGVGQ
                     AAFYFLFNIYSGHATDLGPTVVTAYQVGQFLFGLLGAFFYMQRKEKAPLETSPS"
     gene            complement(10574..11173)
                     /locus_tag="VIG33_11545"
     CDS             complement(10574..11173)
                     /locus_tag="VIG33_11545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEY8271513.1"
                     /translation="MSKSERSLEGFGQQSCLFLDRDGVIIEDIPYNADLTKVALKPGI
                     LQLLRKAHGLGMWITVVSNQSGLGRGYFSWTAYRQIHQKICQLLAAQGEWVDLSLCAP
                     YSEGTDFKQAQARPHYRKPDVGMFKHAEEELGISFSSSVMVGDSATDMMPAFLSGVRK
                     LYLVTSEKQEGEIRKLYEFQKLHPDFKYQVITHFSEVSL"
     gene            complement(11173..11781)
                     /locus_tag="VIG33_11550"
     CDS             complement(11173..11781)
                     /locus_tag="VIG33_11550"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY8271514.1"
                     /translation="MDFKAYIRESLEMSAKNMVALSQSAEVLNSFAGAIKTIVESYKK
                     GGCMYIAGNGGSAADAQHISAELVGKLSRDRTPIRAFALTVDSSFLTAVGNDYGYDHI
                     FSRQVMGLMKPEDCFLAITTSGNSNNIITALEACRKLGAKSILLSGKDGGKIKALNLA
                     DHYIIAPGSNTANIQEAHIAIYHTLCFMIEKSLIEDGLVKYF"
     gene            11959..12483
                     /locus_tag="VIG33_11555"
     CDS             11959..12483
                     /locus_tag="VIG33_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HEY8271515.1"
                     /translation="MTLLLKQIFSFFQLLNSETGTNQLASGLACGLILGFSPFLSIQT
                     LLILCLVFFFRIQMGAAFLSAFFFKFVAFIVDPWADQLGREILENVSLRPLFTEMYNM
                     PLVPLTRFNNSIVMGSGLVGLIMVIPAFFIFRVLITKYRATFVARFKGTKIWKAFATT
                     KVYGWYSKYKELYG"
     gene            12489..14465
                     /locus_tag="VIG33_11560"
     CDS             12489..14465
                     /locus_tag="VIG33_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03545 family protein"
                     /protein_id="HEY8271516.1"
                     /translation="MTTEKNKKTQGPIRWNAVIPFLIICALTFIYFHFFFDFNLRKAF
                     EWAGYKSVGAEVNIANLETSFFKASLRIQGIEITDAEKPLQNTIEIGDIRWGMSWDAL
                     LRGKILVNEAMIENIQFNTSRKYAGRVAPPEPPPKPGPGMLEKEAEKLKEEALAKAQQ
                     HYADNMLGDLAAILGGTDAKAQLGKIEGSLVSKKKAQEIEADIKSKQKFWDDKVKTLP
                     QGKDIQALGDRLNKIKTKDFKTPQELQFSLQELDAVLKQGDIYYKQIGSTGQDLEKDL
                     KKIDADIKELDVLIKADIKALETRFRIPSINTKELTRALFNKYLGKYMAKINHYRAMA
                     EKYIPPKYMDKITGKDQGKSEDGDEISIQPHPREKGISYEFGRKNSYPLLWIKKAAIS
                     SQAGMAPKSGNIRGQILDITSNQVLTGRPTVATINGDFPTEQIKDLLLKLVLDNTKAD
                     SLVTYQISVGSYPIAGTDLVTSSDVKISLTSAVGKLNIDGQLKALREFKMNLNNQFTT
                     TNFKISAKDGNIDSILKNTFSSLPIINLIGSASGYLPSISFEVTSNLGSELEKGLRKQ
                     VEVKIAEARKQINAYINQEVGKQREQIDNQVKQVREQVEREVKKIQDQLNTQKKQAEA
                     KVEQAKKDAENQAKKKLGEQGQKAVEDLKKKFGF"
     gene            complement(14667..15308)
                     /locus_tag="VIG33_11565"
     CDS             complement(14667..15308)
                     /locus_tag="VIG33_11565"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8271517.1"
                     /translation="MNKHLKALLIVGSITALILVAVELYKQKTQDNADSIAGPAKGLV
                     QINQELPNFETKTLQGLNIHLAQFKGKVVVINFWASWCGPCIEEVPSLIKLMQAFPRD
                     LELIAISGDSNHDDINSFMKSFPEMNTLSNIHVVWDADKKLSQKYHIYRLPESFLLNK
                     DLKLVKKLSGTIDWHTEEAIAYVKQLILSKQNAISLDSHGAVDTPSETPSEAH"
BASE COUNT         4574 a         3667 c         3027 g         4284 t
ORIGIN      
        1 tcctcccact tggccaacgg caaccagggc cctaccctat ttttggaaat caaagaatgt
       61 tctgaaattt tcttagtgct gagtaaaaat aatacgtcta aaaaagtcaa ttccttgtcc
      121 ggatcatttt taatatgcca acaaaccgcc gtcagttccg gggtgtcttc atctaaaata
      181 aaagcgttat tggtattttc ctgagaattc tccacagaaa gaaggcccaa cacaatatgg
      241 cttttctctt cactcgcgtt gatttcctcg cttccttggt ctgaaaaatc tggatgttgt
      301 ataagcatga cccactcaga aagctggtcg tgaaaaatca tatcaaataa attaatctgt
      361 ccttttctta ccgcttccat gatttcttgc tctgtcagcg gaccaaaatt ttcttgacca
      421 ttcgaaatgt aatatttttt caccagatct ccctcttatg accattcttt aatttcatca
      481 caattgtgaa aactcctgaa agaataaaaa gaaatctttc tcattatatt ttttcataaa
      541 aaaatactga gctccaggct tcattttctt ctgcgactct tcaatcgcga gcgttcgcaa
      601 acactccagg gtaagacgct tttctgccat acaatgcgaa gagacttgtt ctcgttttgc
      661 aaaaggaacc cacgttacat tgaaataatt ctctccgagc ggccgtattt taagattgat
      721 aagttcattg ggggttagtt cgttcgcttt atccatgggc aagagaagcc aacaaggctt
      781 ttcgcaggta cgaatttctt ctgcactcgc cgtctccgca tttgctagga catgcacagg
      841 ctggcgaaat attttttcgt actcagcctt aagcaattcg aaaatttttt gagactctgg
      901 ggaaagtggc aaaaatcccc ataacacata atgtgcattt tgaaattccg gggacaaccg
      961 aaaaacaact ccctctgcga tattttgccg atctggagaa atcactgacg cagcaatcag
     1021 gggagcagag cgcaagctca cacccacctt cactaaataa agcatgcaag ctgaaattaa
     1081 aataaatgcc aaacagagca gaatggtgat ttttttcata ttctaaatat gaagaaaaag
     1141 aattaagatg acaacctctc tctaaaactc aatcaaacgc gtgttctgct gcgtacccaa
     1201 aggcgcaatg gtgtcagctc tctctcactc cagccaacaa agttgccaac aattttatgc
     1261 tgaccgtcct gtgaaggcaa ttcttcagtt gatacgatcc gcagtgtcgg catcggaata
     1321 ccaattgctt taaatacttc gctatgaact ttgatcagag ctcccgccgt tgcagagata
     1381 ttcgattgca aagtacaacc caattcggac aaagaaatga tctctgtttt agtctcaaat
     1441 tcagctttga ccttaacagc cgcttcgaca aaacgtgagg ggccttgctg cgccttatcg
     1501 acgatgtttt taactttctc taaaagcaaa gatggatcga tcggcttaac cacataatcg
     1561 tcagctcccg cctgaattcc cttttccaca tcggcctttt ccctctttcc agtcagcaaa
     1621 atcaccggaa gccgagccaa ctgaggctga tttcttatag tgcgcaataa agccaaacct
     1681 gtgcccccta cagacatgac cacatccgag atcaccaaat caaagggctc ttgttttaaa
     1741 tgctccatcg catcaatcgc attcgtacaa gcagttacat aatgatgttc acttttaagc
     1801 aaagcttcca aagttaacaa aacgctttgt tgatcgtcga caataagaat atgcgccata
     1861 aaaaaactcc gtttacgccg ccgcacgggt cgcatatttt tgcaactcgc gcaggctttt
     1921 cgtcagttga tcttccactg tcaccaataa taatttaact tcttcaacac ttgcagcgtc
     1981 ttcaatagtt tggcagagct ttgcaagacc tgtcgcccca agagtggcac tcgttgattt
     2041 caacgcgtga gccgattctt gcactccttc tagattgttt tcatgacagc ctgatgcaat
     2101 agttcgcacc aaatctggag ccgtattcat gaaatcctca atcaaagctt ccgtaagact
     2161 tttattgttg accataaact tccgcagctt ttcaagagcc gcttcatcta acactttcgg
     2221 cgaggatttt aaccagcgat gcagaacttc cgtgagacgg tctagctcca gaggcttggt
     2281 aagaaaatca tccatgccca ccgagaaaca aatatttctc tcgccactgg ccccctccgc
     2341 agtcagcgcc acaatcggcg ttttttcacc cttctgtctc tcgcgaattt tttcggtggc
     2401 ctcatatcct gtcattactg gcatttggca gtccataaaa ataagatcat aggaatttga
     2461 cagagccttt tccagggcga tctgaccgtt gttcgccaca tccacctcta aacctaattt
     2521 ctgcaaataa gtcaccgcca cgcgctgatt gactagttga tcttcagcca aaagaacaga
     2581 gccattaaaa accggggcaa agtgactttc atgttgaatc gaaatttgtc ctaaggtggt
     2641 ttctggcaaa tccagagtaa accaaaaagt agtgcctaag cctaatttac tgtgaagtcc
     2701 aatttgtccc cccattagtt ctataagctg acgacaaatt gaaagaccca gccctgtacc
     2761 tccatatttc atacccgttt tcgtttgaaa atatttttga aaaagtgact gactctcttc
     2821 ttcagtcatt cccgcccccg tgtccatgac ggaaatctta agggtggctt tgtttgcata
     2881 ggacttcata ctctccactg caatttgaat gtaaccacgc tccgtgaatt ttaccgcgtt
     2941 acctataaga tttaccaata cctgtcgcaa tctttcagga tcggcaacaa aggcccactc
     3001 ttctcggccc agaaaactga tctccaaacg aatgtttttt tcccgtaccg ccggaaaaaa
     3061 cagttctaga acatcttcac agagaaattt taagtcaaac ggacggggat ttatttgaat
     3121 tttcccagct tctatctttg aatgatcgag tatatcgtta atcagtctca acaaagcttc
     3181 cgcggatctt ttgatgatcc caacgtaatc ttcttgctct tctgtcaatt tggtctccgc
     3241 caataaagtg gacattccta taacaccatt cataggagtg cggatttcat gactcatggc
     3301 tgctaaaaaa tcggatttca tgcgagccgt ttccaaggcc gactcgcggg cacggagcca
     3361 ttcttgctcc gtggctttcc gtcgacttat atctcgaaat acaacgagag cgccgtgaat
     3421 gcccccatca atgtcgcgaa gataacggct gctaatactg atataaattc cctcaggatt
     3481 catttcgttg tggacaagaa tctcaatatc ctcgacctgt tcaccctgaa gagcccgtgc
     3541 gaagggaagc tcctgcagac cgatttgctg taaactattg atgtcaaaaa aaccgaaggc
     3601 tttggcatcc tcttctgaag aaatactttt tattttattt ccaatgattc gttgagccgc
     3661 cgtattataa cgggtgaatt gtcctttttc atcaacaacg atcagggctt cgctaatgct
     3721 attaagaatt ccatccaaaa gaatggattg aattttaagt tccgatatat ctttcttttg
     3781 atcttcatat gccttctccc ttagatagtg actcatcaaa atcaaacaac aagaaatcgc
     3841 aattcccatc aagaaatacc aaagggcttt tggctgagtc atgccaaaaa aattccacgg
     3901 atcagaggaa ttctcactga tatctttaat cattccgtct aaaagtgaaa tcaattcact
     3961 cgactcagat gtcgccgcga ccgcacggtc cccctttatg gtcccgcttt gctttctctc
     4021 aatcttgtca ttgagactca gaaaataagc gcgagatttt ttctgaatct cgacgaattt
     4081 tttatattgc ccaggaactt tcttaaaacc gtccgccaac tcactaaagc gctggccaag
     4141 ctgaacgatc tcccgattgt aatcatccag atctttgccc ttaccattca ctaaatattg
     4201 tgcactattc ctagaagcct gataaatgcc ctggcgaacc tcaaaaagta aggacaaagt
     4261 ataaatgtca gacttagagc cgttgtcttg aaaataatag aagctggacg caaagaggag
     4321 cagaactcca aattgagttc caaaacgtcg caacaaaaaa tggaataacg aagacttatc
     4381 catcaatatc gtatcggaaa aaatatgtct tacttcaata tatccgaacc atatccatac
     4441 tttggtctag ctctaaatac ataaactgat aaataaatta gaaggtaatc gaactttctt
     4501 ccgtctcagg cttatcgccg atcataattt tacgaacgac tcgaatcacc acgcggcgat
     4561 ttttagcccg accttgatag ttcccattat tcgcgatcgg acggctttct ccgtatccga
     4621 tcccttgaaa gtccactttc gcaagttcca gcatgtgctc tttcaaatag gtcttcacta
     4681 cattggctcg ggattgactc aatttttgat tgtaagactc agacccaaca gagtccgtat
     4741 gcccctcgat cgtgatggat tgcacccctg gagtctcgcg aatcattctt accacaatat
     4801 ccaaattctt gtacgagctc ggatcgagtt ctgtggaatc aaatttgaac cttaagaaag
     4861 tcagggtaaa gttttgaact tttgggatct tcgtcaaatc acgtcgaggt tgaacctcgc
     4921 ttctcttttc ccatacggga ccaaatgcat aattgattcc tgaataaatt ctccaggcag
     4981 gggtgccggt cccatgataa atctccgtac ctccgccaaa atgcaccgct agatttgtcg
     5041 tccaatcgta tttcatgccc acaagggtct ctaaagaaga tctctcgcga tccgaaatat
     5101 tcagtgtttg ggaatcattg cttttgccat ccggatagct tccgtaaagt tcccaaatca
     5161 gcttcgtatc taaactggct aaaagataac ttgcggccac cgaataaatc cactgattcg
     5221 gcaatggggt aattccatag gtcgcagcaa gactggctcc tggattgcgc caacgatgac
     5281 caatattaaa accgagcaga acattatgca acatcacgtc ggccaccagc tcaagattgt
     5341 aagtagggcc cggattttcc cctgtataag gattattctc caccaaactt tgatccacag
     5401 aagccactgc ggcccatccc catctatcac cattaaaaaa acgatactta gaattccaac
     5461 gttgtcccgt cattccggtt tggctgtatt tacctagctg accgccatca tccaccgact
     5521 gagaccacaa ataaggaaga gcaaaaccca tatcccaatt atcgccaagg ccgaccccaa
     5581 aattaagatc ggaagagaac aaacgatcat taaacttggt gcgtgcctgc ccactgccat
     5641 tgatcccata cacgtaaggt aaggaattca ctgcatagtt tagaaagaac ccaatattaa
     5701 cgatacctgg atctaaggtg gctgaagaat gaaccgttac aaaatcgagt ccattggtcg
     5761 taggattgaa agtctgcgag tccatgccaa gcacgttggc ttgggagacc tgactttgca
     5821 tgcaaacact actaaaaatt aataggttga ataatatttt gcaaaagttc atgtggaaat
     5881 tatcactaaa cttttactca tcagtcacga cttatttgtt tatgttctct tatagtcatc
     5941 ctgatatcga acaatatcat cttctccgaa ataattccct aactgcacct cgacgaactc
     6001 caaaattgaa tccgaagtat tacgaatacg atgtttagct tggatcggga taaaaacatg
     6061 gcttcccgcc ctcaccagga tgacttgatc atcgaggacc acttccccct gcccccgagt
     6121 gataatccaa tgctcagctc tttgactgtg agattgatag gaaagttggg acataggatt
     6181 caccgtgata actttggatt taaagtgcgc ggtatcacgc agaatctcaa aagcacccca
     6241 aggacgtttt tcaaaaacat gatctttcgt aagcattgaa ccttgcttct gcaaagcttc
     6301 aactacttgc ttcacctctt gggattctcc ttttttaacc accatcaaag cgtctgccgt
     6361 atccaccaga atgagatcat cagttcccac aaaagagtat actttgcctt gtctaccaaa
     6421 aacaaagttg tgatggccgt tcacttctaa ggatttactg gaagcctgtc cttccgccac
     6481 ttgcgcaacc gcatcccaag aacctacatc actccacttc atatcggcag gcacacaggc
     6541 caactcttca gaagagagac tctccataat ggcatagtcg agagaaatat tcttgaactg
     6601 agagtaaatt tcattcaagt tgctaaaatc ttccttcaat tgactgacaa tttcaaaaat
     6661 atcaggctga agattttgaa acttctccag catgatcttg gctttaaaaa taaacatgcc
     6721 tgcattccag aaaaaatcac cggaagctaa aaatttctcg gctttttcca aggaaggctt
     6781 ttcgtgaaac ttcaaaactt tgtttgattg agaatctttt tgaatatatc cgtaaccggt
     6841 ggcgggatga tctgggggga ttcccaaggt tacaatttta ttctgcaaag ccaattgctc
     6901 agcctctcgc accgcttgat aaaaggcttc tgtatcctga attaaatggt ctgatggaaa
     6961 tacacccacc acttcgttac ctaagccacg caagaccaat aaatgagcca agagggccac
     7021 ggcaggagcg gtatttttgg ctacaggctc atagatcgtg gtgacagctg agagatgtaa
     7081 ttctttgaga tttgattccg tcagggtttt taaagactcc ccagtgatga tccaaagtgg
     7141 gcctaaactc tgacatcttt tcaaagtaag gctttgtaaa gaatcttcaa aaagatcaca
     7201 aaactgttta ggaaaatttg cacgggaaac gggccacaga cgagtcccac tccctccgga
     7261 taaaataact ggaatcaaac aattcctcct aggacaaagg cctataaaat caaaagctct
     7321 ggtcatcgtc tactaaaaat agtcacatat tgattcagat ggtcattcaa tctgagtctt
     7381 attcacgaca gtgttgaaga aaacacacgg aaatctttcg gaacacccct gagtttagac
     7441 tgtttttccg cgcgaaaaat tagactttct ttaacgatag agacatactc gaatcttcat
     7501 caggaggatt aattcatgag tattccattt attgacctga aaacacaata caaggccctt
     7561 aagggctcta tagactccca aattcaaaaa gttcttgatc atggagccta tgtcaacggc
     7621 cctgaagtcg ctgaactcga gaaaaaattg gcgcaacaca cgggtgcaaa atatgctctc
     7681 acttgtgcca gcggtacaga tgctctagtc cttcctttaa tggccttagg agtaggcccc
     7741 ggagatgagg ttattacaac agccttctct ttcattgcaa cagctgagac catttgccta
     7801 gtcggcgcaa aaccgatcta tgttgatatt gatccgctga cttacaatat tgatttaaaa
     7861 gcgatagaaa aagccatcac tcctaaaacc aaagccatta tgcccgtctc tttgtatgga
     7921 caaatggcag acatggacga aatcaacgcg atcgctaaaa aacacaacct cttcgtgatt
     7981 gaagatgcgg ctcaaagcta tggagcaagt tacaaagatc gcaaatcttg caatgcctct
     8041 cttgtcggcg ccactagctt ctttccagca aaaccgctgg gatgctacgg agatggcggc
     8101 gcgattttca ccaatgacga aaacctttta aagaccatga aagagattcg cgagcacggt
     8161 tctgaatccc gctattacca cacccgttta gggattaatg gacgtttgga cacactccag
     8221 tgcgccatcc ttttagcaaa aatggaaaga tatgactggg aagtcgaaca aagacaaaaa
     8281 atcgctgacc gttataacga ggccttttct gccatcaaaa ctcctggctt cgtagtccct
     8341 ttagtgaaaa acgatcgcaa gagtgtttgg gcacaatata cattgtccgt agaaaaccgt
     8401 aacgatttcc agaagaaact cacagacacc ggcgtaccca cctctgtgca ttatcctcgc
     8461 acgatgccag atcaaccttg gtacaaagaa aacacacctt gcaacagcaa cattagcaaa
     8521 gctcgccacg ctgcagagca tgtgattagc ttgccaatgt ttccagatat ggatacagca
     8581 actcaaaata aaattattgg cgccgttgtt agcgcaatga agtaaggatt tttcatgtct
     8641 acgggtttta aagctcttga aaaaacagtg actctgcaaa cttctcaagg gtccatcact
     8701 tggggagacg gtagaaattt cgttctcttt gccggtcccg atatcattga agacgaagga
     8761 atggttttag aaacgggcaa agaaatccaa agagtcacca aagctttggg tatcccgtgg
     8821 attttaaaat gctcttttga taaagcgaat cgtcagagtg ccagcagctt tcgaggtcct
     8881 ggctcaaata atgcgcttaa aagccttgat aagattaaaa acgaattgag ctgccccctc
     8941 ttaacggacg ttcatgagac cttccaggtt gaagacactg caaaggtggc ggatgtttta
     9001 caaatcccgg cctttctttc ccgccaaaca gatttgttgg tggcagcggc taaaactcaa
     9061 aaagtcattc atattaaaaa gggtcagttt ctggctccct gggatatgaa ggccattgcc
     9121 aacaaagccg tagccgcagg gaatagcaag ctcctgcttt gcgagcgcgg gaccaccttc
     9181 ggctacaatc gtttaatcaa cgatatgact ggcctcattg aaatgcgtaa gcttggtttc
     9241 ccagttatta tggatgccac tcactctacc caattaccag gggccactgg cgaaagcagt
     9301 ggcggccgcg gagaaatggt ctggcctttg gctcgagcag cgatggctgt cggagtggat
     9361 ggagttttct tggaaactca tcccaatcca gaggtggctc tttgcgacgg cccgacctca
     9421 cttcccctga aaaatctaga agccatgctc aagaatttgc aaaaaatctg gcaaactcac
     9481 ttctagtcta aagagtcgaa acgcatgcaa aaaaaagtta aaaatatttt gttccaaatt
     9541 ctaaagattg ttttctccgt aggccttatt ttctggcttg tccagtcggg caagttgaat
     9601 ttcaaagccc tcaccaatct tttgcatctc aaatatgtgc tcccgggatt tttgctggtt
     9661 gctggcagcg tgtatctgac aagtgaacgc tggagaattc ttttacagac tcaaagccat
     9721 cacctgccct cttggaagac ttttaaactc accatgatcg gagttttctt taacttcgcg
     9781 gttccagggg gcgtgggcgg agatatcgtc aaagctttct atttcaccaa agactatcct
     9841 gaatccaaaa tggcggcggc caccagtgta cttgtagatc gtgtacttgg actctatggt
     9901 ctaattctta tggcctttct tgcaatgctt tttgactgga agcatgtaca ggccgatagc
     9961 tccttacatt ttctttggat agccatcata gctttgctct ttaccgcctc aaccgctttt
    10021 gctttaatat tttcgcagaa aatttatgat cgcggatctt tcagaagagc acttgaaaaa
    10081 cttccttttc acgaaaaaac agttcggatt tatgaatctg ttcatctcta cggccgcaac
    10141 tacaaaactc ttatcggcag tattttcatc agtctcttgt cccagtcctt ttcaatcctg
    10201 tttatgattt tggcaggaca tgcctctgga cttggcactg aaatgccatg gtccacatat
    10261 ttccttgtgg cccccctcgg ttttatggct acagccattc caatttctcc ggcgggagtc
    10321 ggcgttggcc aagcggcctt ttacttttta tttaatattt atagcggcca cgcgacagat
    10381 ttaggaccga cagtggtaac cgcctatcag gtgggacagt tcctcttcgg cctcctcggc
    10441 gcttttttct atatgcaaag aaaagaaaaa gctcctctag aaaccagtcc gagctaataa
    10501 aaataaacca taaagatatt gtccattgaa ccagttccat gagctggttc aataaactat
    10561 cgtttggcaa taatcagagt gaaacctctg aaaaatgagt gatcacttga tatttgaaat
    10621 caggatggag cttttgaaac tcataaagtt ttcgaatctc tccctcttgt ttttctgagg
    10681 tcactagata aagcttacgc actccactta aaaatgcggg catcatgtcc gtggcactat
    10741 ctccgaccat gactgaggaa gaaaaagaaa tccccaattc ctcttcggca tgtttaaaca
    10801 taccaacgtc gggcttacga tagtggggac gcgcttgggc ttgtttaaaa tcagttccct
    10861 ctgagtaggg ggcacagaga ctcaaatcca cccattcacc ttgggccgcc agcaactgac
    10921 aaattttctg atgaatttga cgatacgctg tccaagaaaa atagcctcgc cccaggcctg
    10981 attgattgct caccaccgta atccacatac caagaccgtg agcttttcta agaagttgga
    11041 gaatgccggg ctttagagcc accttagtga gatctgcatt atagggaata tcctcaatga
    11101 tcacgccatc ccgatccaaa aacagacaac tttgttgacc aaaaccttct aaggatcttt
    11161 cgctcttcga cattagaagt acttcaccaa tccgtcttca atgagagatt tctcaatcat
    11221 aaagcacaaa gtgtgataaa tcgctatatg ggcttcctgg atattcgcgg tgtttgaacc
    11281 cggagcaata atataatgat ccgctagatt cagggctttg attttaccac cgtctttccc
    11341 tgaaagaaga atgctctttg cgcccaattt acgacaagcc tctaaagccg taatgatatt
    11401 gttcgagttg cctgaggtcg tgatggcaag aaagcagtct tcaggtttca tgaggcccat
    11461 cacttgccgt gaaaaaatat ggtcataacc gtaatcgtta cccacagctg tcagaaaaga
    11521 actatccaca gtcaaagcaa acgcacggat aggcgtacga tcacgtgaga gcttacctac
    11581 caattctgca gaaatatgct gggcatccgc tgcggatcca ccattccccg cgatatacat
    11641 gcacccgccc tttttataag actccacaat cgttttgatg gcaccagcaa agctattgag
    11701 aacttctgca gattgagaca atgccaccat atttttggcg gacatttcta aactctcacg
    11761 aatataagcc ttaaaatcca ttaccacctc acagtaaaac ttatgttacc aaaaaagctc
    11821 tgcggaagca tctgggtttt tccagaagcc ctctctctac tcactgcgaa aaatcaaaag
    11881 attgccaatg cttagagctc tttctaacct aagcaaatag gtacttaaac tatttttata
    11941 aaacacgtca ggagagttat gaccttgctt ctaaagcaga tattctcttt ctttcagctt
    12001 ctgaattcag aaactggaac caaccaacta gcctcgggcc tcgcctgtgg gctcatcttg
    12061 ggcttctctc cttttctgtc cattcaaacc ttgttgattc tatgtctggt ttttttcttc
    12121 cgcattcaaa tgggcgccgc cttcttgtct gccttctttt ttaaatttgt agccttcatc
    12181 gtcgatcctt gggcggacca attgggccgc gaaattcttg agaatgtatc actgcgtccg
    12241 ctgtttacgg agatgtacaa tatgcctctt gtgccgctga ctcgattcaa taactccatt
    12301 gtgatgggct cggggcttgt cggtcttatt atggtgattc cagctttttt tatttttcga
    12361 gttttgatca ctaaatatcg cgcaactttc gttgctagat tcaaaggcac aaaaatatgg
    12421 aaagccttcg ccactacgaa agtgtatgga tggtattcta aatacaaaga actctacggg
    12481 taatgacgat gacaacagaa aaaaataaaa aaactcaagg tcccattcgc tggaacgccg
    12541 ttattccctt cttaataatc tgcgctctga cttttatcta tttccatttt ttctttgatt
    12601 tcaatttgag aaaggctttt gaatgggcgg gctacaaatc cgtcggagcc gaagtcaata
    12661 tcgcaaacct tgaaaccagt ttcttcaaag caagccttcg cattcagggc attgaaatca
    12721 ctgatgccga aaaacccctt caaaatacga ttgaaatcgg cgacattcgt tgggggatgt
    12781 cgtgggacgc tcttcttcgg ggtaaaattc ttgtgaacga agctatgatt gaaaatatcc
    12841 aattcaatac ctctcgtaag tatgcaggca gagtggctcc tccagagcct cctccaaaac
    12901 caggtcctgg tatgcttgag aaggaggcgg agaaactcaa agaagaggcc ctagccaaag
    12961 cccagcagca ctacgccgac aatatgctcg gcgatcttgc agccatctta ggcggcacag
    13021 atgccaaggc acagctcggt aagatcgaag gcagccttgt ttcaaaaaag aaagcccaag
    13081 aaatcgaagc tgatataaag agtaagcaaa agttctggga tgacaaagtt aaaactcttc
    13141 ctcagggaaa agatattcaa gctttgggcg accgcttaaa taaaattaaa accaaagatt
    13201 tcaaaacccc tcaagagttg cagttctctt tgcaagagct tgatgctgtt ttaaaacaag
    13261 gagatatcta ctacaaacaa attggctcca ccggtcagga ccttgagaaa gatcttaaga
    13321 aaatcgatgc ggatattaaa gaactagatg tattaatcaa agccgatata aaagctttgg
    13381 agacccgttt ccgtattcct tccatcaata cgaaagagct gacacgcgcc cttttcaata
    13441 aatacctcgg caaatatatg gccaagataa atcattatcg tgcgatggcc gaaaaatata
    13501 taccgccaaa atatatggat aagattacag gaaaggatca agggaaatct gaagatggag
    13561 acgaaatatc tattcagcca catccacgcg agaagggaat ttcttatgag tttggccgca
    13621 agaattctta ccctttattg tggattaaaa aagccgcaat tagttcccaa gctggaatgg
    13681 cgcccaaatc tggaaatatt cgcggccaaa ttctcgatat tacgagtaat caagtgctta
    13741 ccggacgacc tacggtagcc acaatcaatg gagactttcc cacagaacaa atcaaagact
    13801 tattgctaaa attggtcttg gataacacca aagcagatag cttggtcact tatcaaatat
    13861 ccgtaggatc ttatcccatt gcagggaccg atctggttac ttcctctgat gtaaaaatca
    13921 gtcttacgtc agctgtgggc aaattgaata tcgacggcca acttaaggcc ttaagagagt
    13981 tcaaaatgaa tctcaacaat cagttcacca cgactaattt caaaatcagt gcaaaggatg
    14041 gaaatataga cagcattttg aaaaatacct tctcaagtct tccaattatc aatctcatag
    14101 gttcggcttc tggatattta ccaagcatca gctttgaggt aacttccaat ttgggaagtg
    14161 aactggaaaa aggacttcga aagcaagtgg aagttaaaat cgctgaagca cgcaagcaaa
    14221 tcaatgctta tataaaccaa gaggtcggta aacaacggga gcaaatcgac aaccaagtga
    14281 aacaagttcg ggagcaagta gagcgagagg ttaaaaagat ccaggatcag cttaacaccc
    14341 agaaaaagca agcagaagcc aaagtcgaac aggccaaaaa agatgcagaa aatcaagcca
    14401 agaaaaagct cggcgagcag ggccaaaaag ccgtcgagga cctaaaaaag aaattcggct
    14461 tctaaccctc ttaaaagaac tcagccgtac caaaggtttt tattcacaaa aataaaaaag
    14521 cgttagaaat agcgcttttt tatttcaaac taaagattct ctagttgcct tacaacaaag
    14581 gcggagttga gctggcgact aaatcctaca gggggcagct taatgccgac accacgatca
    14641 ctattacttt tgctaaggta cgaatttcaa tgggcttcgc ttggtgtctc gctcggagtg
    14701 tccacagctc cgtgcgagtc taaggaaatg gcattttgtt tggaaagtat cagttgctta
    14761 acataagcaa tcgcttcctc cgtatgccaa tcaattgtgc ctgaaagctt ttttacaagt
    14821 ttaaggtcct tgttaagcaa gaaagactcg ggcaaacgat atatatgata tttttgagag
    14881 agttttttat ctgcatccca aactacatga atattagata aagtattcat ttcgggaaaa
    14941 gatttcataa agctattaat atcatcatga ttggaatctc ccgatatagc gataagctca
    15001 aggtcacgag gaaacgcctg catgagttta ataagggacg gaacctcttc aatacaaggc
    15061 ccacaccatg aggcccagaa gttaataaca actactttac ccttaaattg agcaagatgg
    15121 atatttagtc cctgcagggt cttcgtctca aagttgggca gctcctggtt aatttgaact
    15181 aatcctttag ctgggccagc aatactatcg gcattatctt gcgttttttg tttgtataac
    15241 tcaactgcaa ctaaaattag cgctgtaata ctgcctacga taagcaatgc cttaagatgt
    15301 ttattcatta ttaagccctc tcttagcctc aaatacaaaa acctaggttt ccctaggctt
    15361 atgttaatca ctttttaaaa gattgggagc ctaaaattaa actcttgctt cggatctact
    15421 tttcttctga agggtcctaa cagactttct cttagttgcc acagttttag cagcagcttt
    15481 cttcgcctta gtagctaatt tttttgcgcc cttttcatta tctttagcag cagaagactt
    15541 ttcgacttta tc
//
LOCUS       DATBTE010000310         2776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2022186, whole genome shotgun sequence.
ACCESSION   DATBTE010000310 DATBTE010000000
VERSION     DATBTE010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022186"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VIG33_11570"
     CDS             <1..278
                     /locus_tag="VIG33_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8271518.1"
                     /translation="GRLAYLNYGDFKLVLAEDHDLTAPNQKPEIYKTKGRISIHFDTK
                     NVDETFSKIKDGPYVITHPENTPWGTRWFVVEDPDGNQFGWQGPEKK"
     gene            877..1599
                     /locus_tag="VIG33_11575"
     CDS             877..1599
                     /locus_tag="VIG33_11575"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8271519.1"
                     /translation="MKALKTHVAGVDVHKDILAITIMIGSGDEDPKVEHLECSTMTDD
                     LRACGIVLLEKGVRDVAVESTGQYWKPVYNVWEPLGLKVIVGNASHIKNVPGRKTDVK
                     DSEWIAGLHRYGLIRPSFVPEGIYQRLRLLSRHRTNLVGDLGRVKNRVEKVLQDGNIK
                     WSSIVSDTFGVAGVKILDLIADGVTNAQTLAASVTTRIKRKEDVVRALTNCLMNEHIF
                     VLQRLMDNIDISRLKFLRWRTR"
     gene            1596..1820
                     /locus_tag="VIG33_11580"
     CDS             1596..1820
                     /locus_tag="VIG33_11580"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY8271520.1"
                     /translation="MTEKMLPYAPLIEELDKIPGIDKVLAMAIIAGATTDMGSFPDER
                     RFAAWAGVAPGNNESAGKKKEQNADTVILI"
     gene            complement(2122..2211)
                     /locus_tag="VIG33_11585"
     tRNA            complement(2122..2211)
                     /locus_tag="VIG33_11585"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2175..2177),aa:Ser,seq:tga)
     gene            complement(2273..>2776)
                     /locus_tag="VIG33_11590"
     CDS             complement(2273..>2776)
                     /locus_tag="VIG33_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEY8271521.1"
                     /translation="SPCFRSEAGSAGRDTKGLIRQHQFDKVELMTFAHPDQSYEIHEL
                     LTTHAEQVLVDLELPFRRVLLCSGDMGFGAAKTNDLEVWLPGQNTYREISSCSNFEDF
                     QARRANIRFRSTGGKPQFVHTLNGSGLAVGRTLVAILENYQREDGSVVIPKALQNYMG
                     GRKEIRK"
BASE COUNT          781 a          613 c          662 g          720 t
ORIGIN      
        1 ctggccgatt agcttatctc aactacgggg attttaagtt agtccttgcg gaagaccacg
       61 acttaaccgc tcctaatcaa aagcctgaaa tctacaaaac aaaaggacgc attagcattc
      121 actttgatac aaaaaatgtt gatgaaacct tttcaaaaat taaagatggt ccttatgtta
      181 tcacccatcc tgaaaacact ccctggggca ctcgttggtt cgtagttgaa gaccccgacg
      241 gaaatcagtt cggctggcag ggacctgaaa aaaaataagc ggtagtcagc gcgttttgcg
      301 gcgtccgtat ttttacccct gactgggacg gctccgtcgt gttttccgtc ggccccaata
      361 tggcttaatc acttcacctg agtagccttg ttccgtgatc gggcttaacc atctgccctc
      421 atctactcaa gctacataaa tggaaaactc aaaaacagat ttaaaatgct gttttccatt
      481 tatcccctca tcaatgccta tgaagcttcg ctccaccgtc taggacgtaa gtggttttga
      541 tttcttgaat tttttttcaa gtgaagctct ttgggcgccc cgaaggggcg cgatccgatg
      601 gcttttcttt acaatcctat cctttattgc ctatgaagat cccgttttca agggataaca
      661 aggtcgaatt gtctttggcc gaagtgggtc gcttttgcga gtgaccgact gaggtcggaa
      721 tagaatgtgg ccgagtgagc gctttaaaga accgtcgtat ggagcaatgg tcgttgtgac
      781 caaaatcaca gccacggctt cgctaggcaa attttgtagt gcagctcatt cccttctctt
      841 gaagacaaac tttttgcaac aaagggagag gacgaaatga aagcattgaa aactcacgtg
      901 gctggagtgg acgtacacaa ggatattctt gcaatcacga tcatgattgg gagtggagat
      961 gaggatccaa aagtagaaca cttggaatgc tcaacgatga cggatgatct cagagcttgt
     1021 ggcattgtcc ttctagaaaa gggagttcga gatgtggcag tagagtccac tggccaatat
     1081 tggaagccag tctataatgt ctgggagcca ctgggtctga aggtgatcgt cgggaatgct
     1141 tcgcatatta aaaatgtgcc aggaagaaaa accgatgtca aagactcgga gtggatcgcc
     1201 gggcttcata gatatggttt gatacggcct tcatttgttc cagagggaat ttatcaacgg
     1261 ctacgactat tgagtcgtca tcgcaccaac ttggtcggag atctcggaag agtgaaaaac
     1321 cgggttgaaa aggttcttca agatggcaac ataaagtgga gctcaatcgt ctcagacacc
     1381 ttcggagtgg caggcgtgaa gatcttagat ctgatcgcag atggtgttac caatgcgcaa
     1441 acattggcgg caagtgtgac caccagaata aaaagaaagg aggatgtagt tagggcactg
     1501 accaactgtt taatgaacga acatattttt gttttgcaaa gactgatgga caatatcgat
     1561 atatccaggc tcaaattctt gagatggaga acgagatgac tgaaaaaatg cttccctatg
     1621 ctcccttaat cgaagagctc gataaaatcc ctggaatcga caaagtgctt gctatggcga
     1681 tcattgcagg ggccacgacg gacatgggtt catttcctga tgagcgaagg tttgccgcct
     1741 gggcgggagt ggcccctggc aacaacgagt ctgctggtaa aaaaaaagag caaaatgccg
     1801 acacggtaat cctcatctaa caaaagttcg actgccccag tctttggcgt agaccccaaa
     1861 taaaacgcag cataaatctc tcctcttccg ggatttaaaa aatcccaagt gataaaatga
     1921 aaatcagaaa gaagaagagt gattgatgtt tcgactgcta agattgtagc gattttagaa
     1981 ccctaccaag agcctaccat attttgaaat accgtgaaat atgctgaaat actgaacaga
     2041 gcgcctgctc atgttagttg ttgtgtaacc gtttattttg acttgagttt tgaaacttca
     2101 ctcttaatga ggcacgaaga atggcggaga gcgagggatt cgaaccctcg agccccgcga
     2161 agggctgcca gttttcaaga ctggtgtatt caaccgctct accagctctc cgtcgaccgt
     2221 tttattgggt ttagagaggg tattcaagca gttgttgtgt ttgatgcggc gtttattttc
     2281 tgatctcttt gcgtccgccc atgtaattct gaagagcctt gggaatcacc acgctcccat
     2341 cttctctctg ataattctca agaatggcca ctagtgtgcg ccctacggct aagcctgatc
     2401 cattcaaagt atgtacaaac tgaggcttgc cccctgtgga tctaaagcga atgttggcgc
     2461 gacgagcttg gaaatcttca aagttagagc aagaactgat ctcacgataa gtattttgac
     2521 caggtagcca aacctcaaga tcattggtct tcgccgctcc aaagcccata tccccactac
     2581 acagaagcac acgacggaaa ggaagttcta aatccaccaa cacttgttca gcatgagtag
     2641 tgagcagctc atgaatttca taagattggt cgggatgagc gaaggtcatc aattccactt
     2701 tatcgaactg atgttgacgt attaaacctt tcgtatcgcg tccggcacta cccgcttctg
     2761 aacgaaagca gggaga
//
LOCUS       DATBTE010000311        14932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1544292, whole genome shotgun sequence.
ACCESSION   DATBTE010000311 DATBTE010000000
VERSION     DATBTE010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 14932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14932
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544292"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..1030)
                     /locus_tag="VIG33_11595"
     CDS             complement(194..1030)
                     /locus_tag="VIG33_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271522.1"
                     /translation="MKREFNHNVIEQGQTARHLVSWGCLFAGLMLTLLIFSGALALGV
                     AVGGVGLSEGTTAQRAGIFTGVWFIISAVVALFAGGYFSVRLGKFRNDIVGVAHGCVI
                     ASLFVLLLLNQTATAVSWLSRAATDAASGAASMVGGGLNAAAQNGIVSNMVEDTLGDM
                     SFKSDPDVVISGVAARLVRGDTVSAKNYLARQSGLSAAEVDQRITALRAQVDQATVKV
                     REATASAMKATGWSLFFLILFGAIAAAVGGVLASQVNNRKPLAKTLDEPVPLYGTSTA
                     AM"
     gene            complement(1120..1788)
                     /locus_tag="VIG33_11600"
     CDS             complement(1120..1788)
                     /locus_tag="VIG33_11600"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8271523.1"
                     /translation="MTILEELISPLTFAEFQEKYLCRTPFAAPFKASRFRNLLSWSLL
                     EEILSIGHPDCWLPRQGLLPTESALCQGILTMEQAYKGFSEGRTVVVRHAEKAQSQLR
                     EIAKDFAILFPGLVDVQLYFTPAGEEGFDWHYDIEDVFVLQSRGEKEFRLRPNTITPR
                     PLPEILPKDLHFELEPSRCEIRCLLKAGDWLYIPSGYWHKARAKTPSFHISVGVRSSQ
                     NTSS"
     gene            1918..3579
                     /locus_tag="VIG33_11605"
     CDS             1918..3579
                     /locus_tag="VIG33_11605"
                     /inference="COORDINATES: protein motif:HMM:NF045305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron-transfer flavoprotein:ubiquinone
                     oxidoreductase"
                     /protein_id="HEY8271524.1"
                     /translation="MKPKNTKTILPLKFQPPLPIEKVILKEKPQDPVPLDVIFVGAGP
                     AGLSGAIELAKLIANRRKQDSAFPEIQIGVMDKSQNLGEHCLSGAVVNPVAMKYLFPE
                     LDPQQYPFRGLVKREKTFFLTPQNHIMLPTPPTMRNDGYYVASLSEMVRWLGQQAAAL
                     GVHILTGFPVGALLTDGQKILGVRTVAAGQNRDGTPGSGFTPPADLSAQCVVLSEGTR
                     GLLAQCYQSWKGIKSPSPQIYALGVKELWRVKKPLEEIIHTLGWPLSRRTFGGSFFYP
                     MGADLVALGLVVGLDYQETNTDTHQLLQQMKAHPFFRSYLKGGELMEWGAKTIPEGGF
                     HALPEKLHGDGLLIIGDAAGFVNVPALKGIHYAMQSGIMAAQTIFAGLIAGDLSSKQL
                     SKYDDLIRSSQIVKDLYKVRNMREAFHRGFYSGVIKAGLMTLSSGAFPDPTPNKKSDA
                     HVLKTYTKDAMTQPPGSLSKVDGVFHAGNKTRDDIPNHLLVGQNIPQEVAEFYAALCP
                     AGVYEVKEGRLHINAPNCVDCKATDDLGPRWTPREGGSGTRYQQM"
     gene            complement(3584..4630)
                     /locus_tag="VIG33_11610"
     CDS             complement(3584..4630)
                     /locus_tag="VIG33_11610"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEY8271525.1"
                     /translation="MQSKDFYQSHLDRVSRSFAFCIARLDGDLRIQISLSYLLLRLLD
                     SIEDAIWESPQSQGESFEIFNRFMQESPQESLWQNWVQRVPNTLPLVERDLLVDSLSL
                     FRELHQLPPNVKRIIQDSVLNMSRGMMFFLERRGSKKGLHLDNLDEVNGYCFFVAGIV
                     GELLTDLVALTSQRLMTSPKIYVQAIHFGLFLQKINILKDQLADEKEGRQLVPSRSEL
                     IASLRLNAQGAVEYLVAIPLEEKGYRLFCAWSLFLGLASISWIQKSWILRILEKIPRS
                     ITMKLLANVEVIIDDNVALLKQFQEFVQTLPTLEKNISADQVPWISYEKFQELYSGRL
                     SFEELSEIGLFAPS"
     gene            complement(4687..4953)
                     /locus_tag="VIG33_11615"
     CDS             complement(4687..4953)
                     /locus_tag="VIG33_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271526.1"
                     /translation="MAPQKSPEARPGRSELYKNLSGVSGILRFKINRDSIEVEFHDGA
                     VYLYDEAITGRVHVERMKELARAGKGLNSYISRAVRKNYARKLK"
     gene            complement(5052..6548)
                     /locus_tag="VIG33_11620"
     CDS             complement(5052..6548)
                     /locus_tag="VIG33_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271527.1"
                     /translation="MKRQINHFCMIFFGVSVLLFQNCTGGFHLGSDELTDSTAASSSG
                     VLLYEQSLSELQPYSYKFSGSETVSFQDALPAGAFYDSDTRTLYWIPTKGQRGDYNLG
                     VKEGGVDKFRIAFHVLALDDYKLRYGGPPFHYQDGDVGYIFVHGVQDTDLCIDRSKLE
                     QYWGGTPQTIAPDSNDRSLACYDGRKAVAAVAESVAHQIMDAKCGFYKKCIIITHSMG
                     GLLMEHMFLHIRPPVAIDPVQSWFDQRELYKAVREKTLFVISLASAAGGSKTADLVNG
                     NAGTVSQDVIGALSRWMGANMDSTNNLMVAYASRVVAPMTEDPGVPFFMVPGFTTKTL
                     YEARGFFGSIWDTLINSVPDTVFNGDGDLTFVDGVTEFPSRSDGVVDLRSSCGIASDD
                     YNNGVGDKASLDDNLHYCWGAPKKANHYLWFAVNLNHSLITRPAAEVSECFSQENPCV
                     PRFPASLGNLFPDDTMKSWAAIDVIRNKIAMPRMPAAPVISKGSLAGN"
     gene            7024..8598
                     /locus_tag="VIG33_11625"
     CDS             7024..8598
                     /locus_tag="VIG33_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271528.1"
                     /translation="MRKYVSFTAFVITALVLNTSCTKSGFSLNENGALTRDFSNTGIL
                     NKAKYPTLTISPSSVSLQTNTYQNFTASGGSGGYVFSASSGVITSVGNVATFKSPSIA
                     GSVVVTVRDSRGYRAQSQVNVVNPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTP
                     TPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTPTP
                     TPTPTPTPTPTGANILFKSGFESGVNLGAPYSCGGTQCSQDITGRDSGGSSWPVNFWG
                     SANNSYLQLLSTAPVTTSTISNYMVNEIQTVTGHNGTSTRAFYQSLKGSYGGNKNQQD
                     PYIIGPTNTVAEQGDLYIRYWVKFQSDLATNQLIPGQTPDGGYGNWRVLWEWKTGGQG
                     ANWPGDYRFIVGITMNSSRQLKWYATADNVANSGAPKKVYFHEENSGAPVLAGTWFKF
                     ETFTHRSSGSDGRLWVAINGQVIADFRGANMGVNNFPINRIMLFQNYTGGYYPSYQWV
                     DDVEVFDGFPSDATNH"
     gene            complement(8681..9373)
                     /locus_tag="VIG33_11630"
     CDS             complement(8681..9373)
                     /locus_tag="VIG33_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8271529.1"
                     /translation="MSKILLIDDYQDIGELVTRGLRPYPVTHVYTIGEAEQALKSSSF
                     EVFIIDVNLPDGNGFDFCVSLGQNPLYQNVPKIILSGQGEVTDKVYGLNCGADDYVTK
                     PFDVVELRARVERFLRRLQSPMKNYLNVAGFSFDTEFLKCYVIDGRSKKDLELTPTEF
                     RIFLTLAKSEGRLVTRRALEKAAWEPQGVNIKIRGIDTHIAHLRKKLGAEMHMIVSVY
                     GQGYSFNTQIRG"
     gene            9546..9668
                     /locus_tag="VIG33_11635"
     CDS             9546..9668
                     /locus_tag="VIG33_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271530.1"
                     /translation="MNTESVQQQQHQHHLRTQVLIVDDDIDSALRVEAIFHHLG"
     gene            9757..10449
                     /locus_tag="VIG33_11640"
     CDS             9757..10449
                     /locus_tag="VIG33_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8271531.1"
                     /translation="MLYTPLPFTQILDNAEIQELVEKLPSDSWINNTFHLENNLWCFQ
                     FDCNHVGGSFHATGLGQTPKEAFMVARQIILQQLEQWHKTRFLNLNVCSPPPNILIVD
                     DDVDLALAMQTALTDLGYHVDVANRYENLHHTIAEGNADYIFLDWKLNNNVTAGQVME
                     KTNLLIDTFSDLRHKFELQPPRVVTYSSLDCTETLLPASGKRYFKHVDHWQKPMPFRE
                     AIARASGLLAAK"
     gene            10461..10844
                     /locus_tag="VIG33_11645"
     CDS             10461..10844
                     /locus_tag="VIG33_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271532.1"
                     /translation="MRKFNANNAALYWGSEDEFTDTNEDILSLVPPELTPLLPGFLAR
                     RENEIVELSEFLRLRQFASIKSVGHKMKGTGLGYGFQIITDLGRGLEQAAQDQEIDNI
                     HLLIAKLTVFCKELRARMAIDLSRK"
     gene            10850..12883
                     /locus_tag="VIG33_11650"
     CDS             10850..12883
                     /locus_tag="VIG33_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8271533.1"
                     /translation="MRRLVPFKNQWKNLSVSHKLYAVVGVMAVLIASELFTLLFAMNI
                     LSAVRAFVGGEGLWSKAQKGAVLEIQNYARTRDVKYYEAFREHLKIPLGDHAARLEMQ
                     KTNMDKSIVTKGFLEGQIHPQDIVPMVKLMRRFSEVSYLQKAILAWEKADHQIFQLIE
                     ASERLHKLISQPGKKKASDEEVQALMDKILVINKEATEFENQFSATLGEASRWLESTL
                     MILLIIAVITVESTGLILTITFSKGLTRVLNELNAFAQKVGSGDFSKKVPVRSNDELG
                     HLAAALNKMTEELQGMNSERQVAERSNQIKSLFLANMSHEIRTPLNAILGFADLLKDP
                     SLPRNESRKYLEIIERTGFGLLTIINDILDISKVDAGKLEIEKAPCSPLQLASDLESM
                     LRLRCEEKGITLTFETIELPKRIITDPSRLKQILINMIGNAIKFTSHGGVKVTLFTQA
                     QKLICTVEDTGVGIPVESRHRLFQPFSQVDLSIRKQYGGTGLGLILSRRLAKLMGGDV
                     ILKYSEPGRGSVFEVSIAFEADNTVEVASKRRELPAETSLSLNGKRILIVEDSLDNQL
                     LAQQYILKEGAEVIIANNGLEALRAMTLHTFDLVLMDMQMPVMDGYTATLELRDKGFT
                     IPIIALTANAMKEDLERCLHSGCNDYLCKPFRRGDLLNMIAQHCQNPKNQTVS"
     gene            complement(13061..13435)
                     /locus_tag="VIG33_11655"
     CDS             complement(13061..13435)
                     /locus_tag="VIG33_11655"
                     /inference="COORDINATES: protein motif:HMM:NF017231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271534.1"
                     /translation="MPANGPNLNAPALEIVFDGIYWVKHAQNQDVPAIRNHLSKLWDM
                     TLKSSDITTTQRLIAEFEWWFFVANPTARGAASLGDLLSLSLQISKGLKLRNEFLHMD
                     WFALSRSLDDYVSWRLSLGTKN"
     gene            complement(13509..14462)
                     /locus_tag="VIG33_11660"
     CDS             complement(13509..14462)
                     /locus_tag="VIG33_11660"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY8271535.1"
                     /translation="MIRFANLEDARKFVSRWGGDPSSIEHLGDSGNSVFSFKNSDGKL
                     QILRFTDNEFRSFDEVTAELNFVNHLYGANIPVAQGLSTKDGELVFWAECSTGSLICS
                     SIAYAPGVDVQEGAELWNSEFFKEWGRNLGLIHQAASKYVIKNEEPKRWQWDNEILIS
                     RADDLIPSEDKIAREEFNEVITSCQQLQKRPSEFGLIHADHAPQNFRYDPTGKRLTAF
                     DFGNCCYHWFLSDLAISLSTVRRKKNRDEIRENILQGYSSVYSLPEHYEELIDLFIRL
                     RVVYVYLSRLYLWSRNRTEKQAKELQVIKSRVHTKTGWGQA"
     gene            complement(14459..>14932)
                     /locus_tag="VIG33_11665"
     CDS             complement(14459..>14932)
                     /locus_tag="VIG33_11665"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEY8271536.1"
                     /translation="GHLARANPHWKLIDGLPVTAIFHGPNAYITPNWYAENDVPTWNY
                     AVVHAVGQGRLISDYDGIVACLKNLTAQVEAKSKNPWNFWLPTDLESPADLTSAITGF
                     EIEVKDINAKFKLSQNRSLSDRNGAIAGLESERTDEDSRKISELMKKIGNRASDS"
BASE COUNT         4160 a         3650 c         3272 g         3850 t
ORIGIN      
        1 ctatccgaat gttttcattg gacttctggt tgtactcaaa cagaagtaaa gtcgcgctac
       61 gagtttcaaa atgaataata agctccttcg cgacttatga tagccgtatc ttcgtctagt
      121 agaaaattta agacccttct aaaaaacaaa gagtcggtgg cgacagcccg gaacgaccct
      181 accaaacttc tttctacatc gctgcggtcg aagtgccata gagaggaact ggctcatcga
      241 gcgtttttgc caaaggtttg cggttattca cttgtgaagc taacacgcca ccaacggcgg
      301 cggcgatggc tccaaagaga attaagaaga ataagctcca gcctgtggcc ttcatggcgc
      361 tggcagtggc ctcacgaact ttaactgtgg cctggtcaac ctgggcccgc aaagcggtga
      421 ttctctgatc cacttcagcg gcactgagcc ctgattggcg tgccagataa tttttggcgc
      481 ttactgtgtc gccgcgaacg aggcgagccg ccactcctga aatgaccaca tcaggatcag
      541 atttaaaact catatccccc aacgtgtcct ccaccatatt cgaaacaatg ccattttgtg
      601 ccgccgcatt gagaccgccg cccaccatcg aggccgcgcc actggccgca tccgtagccg
      661 cacgactgag ccaactcacc gccgtcgcgg tttggttgag caatagcagt acaaataaag
      721 aggcaatcac acagccatgg gccacaccca caatgtcatt gcggaatttt cctaagcgca
      781 cggaaaaata gccgcccgca aaaagcgcaa ccacagcgga aataatgaac caaacgccgg
      841 taaagatgcc ggccctttgc gccgtcgtcc cctcagaaag acctaccccg ccgacggcca
      901 cccctaaggc cagagcaccg gaaaaaatca gcagtgtcag cattaagcct gcaaacaggc
      961 agccccaaga aactaaatgc cgagccgttt gcccctgttc aataacgttg tgattaaatt
     1021 cccttttcat aattcctcct gtaaaaatat gtaaatatgt aaagttttac ggaattatat
     1081 taacgttttc tgagggactc cctaaatgat ctttttttct taagaagatg tattttggga
     1141 ggaacgcacc cccaccgaaa tatgaaatga aggggtcttt gcccgcgctt tatgccaata
     1201 tcctgaaggg atatagagcc aatctccggc ttttaacaaa cacctgattt cacaacgaga
     1261 aggctccagt tcaaagtgaa gatccttagg caaaatttcc ggcaagggac gcggcgtgat
     1321 cgtgttcggg cgaagacgaa attctttttc tccgcggctc tgcaaaacaa acacatcttc
     1381 aatgtcataa tgccaatcga aaccctcttc gcccgccgga gtaaaataca gttggacatc
     1441 caccagcccc ggaaagagta tcgcaaaatc ttttgcgatc tcccttaact gagactgggc
     1501 tttttccgca tgccgcacta caaccgttcg gccttcgcta aatcctttat aagcttgctc
     1561 catggtgaga attccctggc aaagtgcaga ctcagtcggc aaaagccctt gtcgcggaag
     1621 ccagcagtct ggatgcccaa tagacaaaat ttcttctaat aaagaccatg ataataagtt
     1681 acgaaaacgc gaagctttga agggcgcggc aaatggcgtg cggcaaagat acttttcctg
     1741 aaactccgca aatgtaagag gcgaaatgag ttcttcaaga atggtcatga gaacctattt
     1801 aatgaattaa gcctcagaat tcaaagagga attaagcgct tgttttgatt ttttcgtcat
     1861 accgtttaca ggaaggcccc attcctagaa tatgaagacg tgaatgaaaa tgattttatg
     1921 aagcccaaaa atacaaagac aattctaccg ctgaaattcc agcctcctct gcctattgaa
     1981 aaagtgattc tcaaagaaaa accacaggac cctgtgccgt tagatgtgat ctttgtcggt
     2041 gccgggcctg cggggctctc cggggcaatt gagctcgcga aactgatcgc gaatagacga
     2101 aaacaagatt ctgcttttcc agaaattcaa attggggtta tggataaatc gcaaaattta
     2161 ggtgaacact gcctttccgg cgccgtcgtt aatcccgttg cgatgaaata tctttttccg
     2221 gaactggacc cacaacaata tccatttcga ggtttagtca aaagagaaaa aactttcttt
     2281 ctgactccac agaatcacat tatgctgccc actcctccga cgatgagaaa tgatggctat
     2341 tacgtcgctt ccctttctga gatggtgcga tggcttggcc agcaggccgc cgcccttgga
     2401 gtgcatattc tcaccggttt tcccgtgggc gccctattga ccgatggaca aaaaatatta
     2461 ggagtccgca ccgtcgcagc gggacagaac cgggatggca ctccaggcag tggattcacg
     2521 ccgcccgccg acctcagtgc ccaatgtgtc gtgctgagtg aaggcactcg cggtctcttg
     2581 gctcagtgtt atcagagctg gaagggaatc aaatcccctt caccacaaat ctacgccttg
     2641 ggcgttaaag agctttggcg tgtgaagaaa ccactcgaag aaattattca tactttggga
     2701 tggcccttat cacgtcgtac gtttggcgga agttttttct atccgatggg agcagatctt
     2761 gtggcactgg gcctggtcgt cggccttgat tatcaagaaa cgaatacgga cacccatcag
     2821 cttttgcagc agatgaaagc gcatccgttt tttagatcct acttgaaagg cggagaactt
     2881 atggagtggg gtgcaaagac aatccccgag ggaggcttcc atgcccttcc agaaaagctt
     2941 cacggagatg gcctcctcat cattggggat gctgcgggct ttgtgaatgt ccccgcctta
     3001 aaaggaattc attatgccat gcaatcagga attatggcgg cgcaaactat ttttgcaggc
     3061 ctcatcgctg gagatttgag ttcaaagcaa ttgtcgaaat atgatgatct catcagatcc
     3121 agccagatag tgaaagatct ttataaagta cgaaatatgc gagaggcctt tcaccgtgga
     3181 ttctattccg gtgtgatcaa ggccggcctg atgactctca gttcaggagc ttttcccgat
     3241 cctactccca acaaaaagtc agacgcccac gtcttaaaaa cttatactaa ggatgccatg
     3301 acacaacccc caggatcttt atccaaagtc gatggggttt ttcatgcagg caataaaact
     3361 cgagacgaca tccccaatca tctcttggtg ggccagaata tcccgcaaga agtggccgag
     3421 ttttatgcgg ccctgtgccc tgctggagtc tacgaggtga aagagggacg gcttcatatc
     3481 aatgctccca actgtgtgga ttgcaaggcc accgatgatc ttgggccacg ctggaccccc
     3541 agagagggag gaagcggaac ccgttatcaa caaatgtgaa tttttatgaa ggagcaaata
     3601 aaccaatttc cgacagttcc tcaaaactca agcgacccga gtacagttct tggaatttct
     3661 cataggaaat ccacggaact tgatcagctg agatattttt ttcgagcgtg ggcaaggttt
     3721 gcacgaattc ttggaactgc ttcagcaggg cgacattgtc atcgatgatg acttcgacat
     3781 tcgccaacaa cttcatggtg atacttctag gaattttctc tagtattcta agaatccagc
     3841 ttttctgtat ccacgaaata gaggccagtc ccaaaaacaa agaccaggcg caaaaaagac
     3901 ggtagccctt ttcttccagg ggtattgcca ctagatactc aactgcgccc tgggcattca
     3961 agcggaggct ggcaataagt tcgctccgtg aaggaaccaa ctgtcgtccc tccttttcat
     4021 ccgccaattg atccttgagg atattgatct tctgaagaaa gagtccaaaa tgaatcgctt
     4081 gaacataaat tttaggtgaa gtcataagtc gttgactggt aagagctacc aagtccgtga
     4141 gcaactctcc gacgatgcca gcgacaaaaa agcagtatcc gttcacttcg tcaaggttgt
     4201 ctaaatgcaa tcccttttta gagcctctgc gttccagaaa aaacatcatg ccgcgactca
     4261 tattaagcac ggaatcttgg ataattcgct tcacgttcgg aggtaactga tggagttccc
     4321 gaaaaagcga gagcgagtca accaacaaat cacgctccac caatggcaga gtgttgggaa
     4381 ctcgttgaac ccaattttgc caaagggatt cctgcggtga ctcttgcata aaccgattaa
     4441 atatttcaaa actttctccc tggctttgag gcgattccca gatggcatcc tcgatagaat
     4501 ccagcaatcg gagaagcaaa taggaaagac ttatttggat tcttaaatcc ccgtcaagac
     4561 gggcgataca gaaagcaaaa cttcggctca cccgatccaa atggctttga taaaaatctt
     4621 tagattgcat cgacaaacta tagagactta ctcatggcca agcaagtctt agtgcctata
     4681 aagagcttac tttagcttcc gggcataatt tttacggaca gctctgctaa tgtaactgtt
     4741 cagccctttt ccagcccgag ccagctcttt catacgctcc acatgaacgc gacctgtgat
     4801 tgcctcatcg taaagataga ccgcgccatc gtggaattcg acctctattg agtcccgatt
     4861 gattttaaaa cgaaggatac ccgagacccc actcaaattt ttatagagtt cggatctccc
     4921 tggccttgct tcgggtgact tttgcggcgc catcacaaag ccctcccaag aaatattatg
     4981 atactaaatt ttaagctgca gagaaaattt taggatagat ttgtggaata gctgtctgct
     5041 ctttttcttt tttagttacc tgccaatgag cctttggaaa tgacgggggc cgcaggcatt
     5101 cgaggcattg cgattttgtt ccggatgaca tcaattgcgg cccagctctt cattgtatcg
     5161 tcaggaaaaa gattccccag ggaggctggg aagcgcggca cacagggatt ctcctgactg
     5221 aagcattcag agacttccgc cgcaggacgc gtgatcaggg aatgatttaa gtttaccgca
     5281 aaccagagat agtgattggc tttcttgggt gcgccccaac aataatgaag gttatcatcg
     5341 agagaagctt tgtcaccgac gccattattg taatcatcac tggcgattcc acaggaactc
     5401 cgcaagtcca caactccatc actgcgtgag ggaaactctg tcaccccatc cacaaaagtc
     5461 aagtcgccat ccccgttaaa gaccgtgtcc ggaacggaat tgataagggt gtcccagatg
     5521 ctgccaaaaa aacctctggc ctcataaaga gttttggtcg taaacccagg aaccataaaa
     5581 aacggaaccc cgggatcttc ggtcatcgga gccacgacgc gtgaggcata ggccaccatc
     5641 aggttattgg tagaatccat gttggctccc atccaccgac tgagtgcccc tatcacatcc
     5701 tgacttacag tccccgcgtt gccattgacc aaatcagcgg ttttagatcc tcccgccgct
     5761 gaagccagtg aaataacaaa caatgttttc tcacgaacgg ccttataaag ttcgcgctgg
     5821 tcaaaccagg attggaccgg atcgatagca accggagggc ggatatgaag aaacatatgc
     5881 tccatcaaaa ggccgcccat gctgtgggtg atgataatac attttttata aaaaccacat
     5941 ttcgcatcca tgatctggtg agcaacgctt tccgccacgg ccgccacggc ttttcgacca
     6001 tcatagcaag ccaaggatcg atcgtttgaa tccggcgcga tggtttgagg ggtacctccc
     6061 caatattgct ccaacttact cctatcgata caaagatccg tatcttgaac gccatggaca
     6121 aagatatatc ccacatctcc atcttggtaa tgaaaggggg ggcctccata tcgcaattta
     6181 taatcatcaa gagcaaggac atgaaaagcg atacggaact tgtcaacccc tccctccttc
     6241 acgcctaaat tgtagtctcc ccgctgaccc ttggtgggaa tccaatacaa tgtgcgagtg
     6301 tcagaatcat aaaaagctcc ggctggaaga gcgtcctgaa agctcacggt ctcagatcca
     6361 gaaaatttgt aactataagg ttgtagttca ctaagggatt gctcgtagag aagcacaccg
     6421 cttgaactgg ccgcggttga atctgtcagt tcatcgctgc caaggtggaa ccctccggtg
     6481 cagttctgaa ataacaatac cgataccccg aaaaaaatca tgcagaaatg attgatttgt
     6541 cttttcactc cagcgccccc cagcaccttc ataactctgc aaatactttt ccagcctaag
     6601 tgcgatgtat gcgcactgaa gcgccaaaaa tatgaacagc tgtctaagga agtgtctgaa
     6661 tatgggggtg gtttaactta acaattggat attcttgaga ttccgagagt gttcacagag
     6721 gagcatgatt gctcaaggag ttgacaacat cacccgtaga cccgacacat ctggcgctcc
     6781 gtttgaactt ccaggctaca aatagacata ggaaagcggt tacttttagc tttgaaaaaa
     6841 gtctgtatca gattgatgca tccgcagagg ctgccgcaaa ttccagaaag atgctcagtg
     6901 ttgtataatt ttttacagtt gttaaaacat tgggacttat ttccattata atttccgtaa
     6961 acatgttaaa atctatttga aaatggtcat ccttttgcag gtctctagat gggggtcatc
     7021 aatatgagaa aatatgtttc atttaccgcc tttgtcataa cagctctggt attaaatacc
     7081 agttgcacta aatcaggctt ctctcttaat gagaatggag ctctaactag agatttttca
     7141 aatactggaa tcttaaataa agccaagtac ccaactctca ctattagtcc tagcagtgtc
     7201 tctcttcaaa ccaatactta ccagaacttc acagcttcag gtggaagtgg cggatacgta
     7261 ttttctgcct ctagcggtgt gatcacttcc gtgggcaatg tcgctacctt taaatcccca
     7321 agtattgcag gctcggtggt cgtcactgtc cgagactcgc gcgggtatag ggcccaatca
     7381 caagtaaatg tcgtcaatcc gactccgact ccaactccga ctccaactcc gactccaact
     7441 ccgactccaa ctccgactcc aactccaact ccgactccaa ctccgactcc aactccaact
     7501 ccaactccaa ctccaactcc aactccgact ccaactccaa ctccgactcc gactccaact
     7561 ccaactccaa ctccaactcc gactccaact ccaactccaa ctccgactcc aactccaact
     7621 ccaactccaa ctccaactcc gactccaact ccgactccaa ctccgactcc aactccaact
     7681 ccgactccaa ctccgactcc aactccgacg ggtgctaata ttttatttaa gtcgggattt
     7741 gaaagtggcg taaatttagg tgcgccttac tcttgcggcg gcactcaatg ttcgcaagac
     7801 atcacaggcc gagattctgg cggaagctcg tggcccgtga atttttgggg aagtgctaat
     7861 aatagttatt tgcagcttct cagtactgca cccgtaacta cctcaaccat tagtaactat
     7921 atggtgaatg aaattcagac tgtcactggc cataacggaa catccactcg ggccttttat
     7981 cagtcactga agggaagcta tggaggaaat aaaaatcagc aagaccctta catcattgga
     8041 ccgacaaaca ctgtcgctga acaaggggat ctttacatcc gctattgggt taagtttcaa
     8101 tcagatttag ctacgaacca gctcattcct ggccaaactc ctgatggcgg gtacggcaac
     8161 tggcgcgttc tgtgggagtg gaaaactgga ggacagggtg ccaattggcc aggggactat
     8221 cgcttcattg taggaataac tatgaattcc agtcggcaac ttaaatggta cgctacagct
     8281 gataatgtcg cgaactccgg agctccaaag aaagtttatt tccatgagga aaattcaggc
     8341 gctcctgtcc tagctggcac ttggtttaaa ttcgaaacct ttacccatcg aagtagcgga
     8401 agcgatggaa gattgtgggt tgccataaat ggtcaagtca tcgctgattt cagaggagca
     8461 aatatgggag tcaataactt cccgatcaat cgcatcatgc tctttcaaaa ttataccggc
     8521 ggctactatc cttcctatca gtgggttgat gacgtagaag tctttgatgg cttcccttcg
     8581 gatgctacaa atcactagag ctagccctag aggaatcttt tataaaatta ccgggggtaa
     8641 tgccccggta aaaaatgtga tttatacttt gaggttcggc ttatccgcga atctgggtgt
     8701 taaatgaata gccttggcca tacacagaaa caatcatatg catttcagca cccagttttt
     8761 tgcgcaaatg agcaatatgc gtatcgatcc cgcgaatctt gatgttgacc ccttgaggct
     8821 cccaggccgc tttctctaaa gctctccgag tcaccaagcg accttcgctt tttgctaacg
     8881 ttagaaaaat tctgaattca gtcggagtga gctcgagatc tttcttggag cgtccatcaa
     8941 tcacatagca ctttaaaaac tcggtatcaa aactgaaacc tgcgacgtta aggtaatttt
     9001 tcattggcga ttggaggcga cgtaaaaatc gctccacccg tgctcgcagc tcaaccacat
     9061 caaaaggctt ggttacataa tcatcagccc cacagttaag gccataaact ttatccgtca
     9121 cttcaccttg gcctgaaaga ataatcttag gcacattttg ataaagcgga ttctggccaa
     9181 ggctgacaca aaaatcaaaa ccgtttccat ctggcaagtt cacatcaatg atgaaaactt
     9241 caaaggacga tgatttcaaa gcctgttcag cttcaccaat ggtataaaca tgggtcaccg
     9301 gatagggcct taatccacga gtgaccaatt ctccaatatc ctgataatca tcaatcaaaa
     9361 ggattttcga catacttcta ttttatcatc ttgtttatat ccagaacacg ctcgttttga
     9421 gaaaatactc attcaaaaca tgattttgtc tcagcctgac acggcaaatg tcgagatcat
     9481 acaaccttct cacaatccaa tcttaaattt ttttttggca aactattcgt atcaggagga
     9541 atactatgaa tacggaaagc gttcaacagc aacaacacca acatcatctt cgtacccagg
     9601 tgcttattgt cgatgatgat attgattcag cactgagggt cgaagcgata tttcatcacc
     9661 tgggctgatc actggcaaaa accaataccg cagcgagaat tattggttcg cgcgctcggt
     9721 ttacttaatc agcttcaccg ctagcaagga ggatttatgt tgtacacacc actgcctttt
     9781 actcaaattt tagataacgc tgaaattcaa gaactcgtcg aaaagctgcc ttcggactct
     9841 tggattaata acacctttca tcttgaaaac aatctttggt gttttcaatt tgactgcaac
     9901 cacgttggcg gaagctttca cgccacggga ctgggccaaa cgccgaaaga agcatttatg
     9961 gttgcaaggc agatcatact gcaacagctc gaacagtggc ataaaactcg atttttgaat
    10021 ctgaacgtct gctccccgcc accgaacatt ttgattgtcg acgatgatgt tgatttagct
    10081 ttagcgatgc aaacagcact cactgacttg ggctaccacg tcgatgtcgc gaacagatac
    10141 gagaatctcc atcacaccat tgctgagggc aatgctgatt atattttcct tgattggaag
    10201 ctcaataaca atgtcacggc agggcaggtc atggaaaaaa caaatttact gatcgataca
    10261 tttagtgacc tccgtcacaa gtttgagctt cagccaccgc gagtcgtgac ctattcctcg
    10321 ctggattgca ccgaaactct gctgccagct tcggggaaac gttacttcaa acacgtcgat
    10381 cactggcaaa aacccatgcc gttccgggaa gcgattgcgc gggcctcggg cctgctcgct
    10441 gccaaataaa ggaggatacc atgaggaagt tcaatgcgaa caacgccgcg ctatattggg
    10501 gaagtgaaga tgagtttacg gacaccaatg aagatatcct ttctttggtg ccgccggagc
    10561 tcactccatt gcttcctgga tttttagcgc gacgagaaaa tgaaatagtc gaattgtcag
    10621 agttcttaag gcttcgacaa tttgcatcca tcaaatcagt gggacataag atgaaaggca
    10681 caggactcgg gtatggattt caaatcatca ccgatctagg cagggggctt gagcaagcag
    10741 cgcaggatca agagatcgac aatattcatt tattgattgc caagctcacg gtgttctgta
    10801 aggagcttcg agcccgcatg gctatcgatt tatcaaggaa gtaatattta tgcgacgttt
    10861 agtgcctttt aaaaaccaat ggaaaaacct gtcggtctcc cacaagctgt atgcagtggt
    10921 gggagtcatg gcggtgctta ttgcctccga acttttcact ctgttatttg caatgaatat
    10981 cttatctgcc gtgcgggcct tcgtgggtgg cgaaggcctt tggtccaaag ctcaaaaagg
    11041 agcggtgctt gaaattcaaa attatgctcg cactcgagat gtaaaatatt acgaggcctt
    11101 tcgtgaacat ttaaaaattc ccctcggaga tcatgcggcc cgtctagaaa tgcaaaaaac
    11161 caacatggat aagtccattg tcaccaaggg gtttcttgaa ggtcaaatac atccgcaaga
    11221 tatcgttccc atggtaaaac tcatgcgccg atttagcgag gtttcttatc ttcaaaaagc
    11281 tattctcgcg tgggaaaaag ccgatcatca gatttttcag ctgattgagg cctcagagcg
    11341 gcttcataaa ctcatcagcc aacctggcaa gaagaaagcc agtgatgaag aagtgcaggc
    11401 attgatggat aagatcttgg tgataaataa agaggccacg gaatttgaaa atcagttctc
    11461 tgccacgctc ggtgaggcct caagatggct tgaaagcacg ctcatgattt tactcatcat
    11521 cgccgtgatt accgttgaaa gtacgggcct gattctgacg attactttta gtaagggttt
    11581 aaccagggtc ctgaacgaac tcaatgcctt tgcccaaaaa gttggatctg gtgacttttc
    11641 aaaaaaagtt cccgtgcgtt ccaatgatga actagggcat ttagcggcgg ccctcaacaa
    11701 aatgactgaa gaactgcaag ggatgaatag cgagcggcaa gtcgctgaac gctccaatca
    11761 aattaaaagt ctctttttgg caaatatgag tcatgaaatc cgcacgccac ttaatgccat
    11821 tctcggtttt gcggatcttc tgaaggatcc ctctctccca cgcaatgaaa gcagaaaata
    11881 cttagagatc attgaacgca cgggctttgg cctcctcacc atcatcaatg atattttgga
    11941 tatctcgaag gtggacgccg gtaaactgga aatcgagaaa gccccctgct cgcctctgca
    12001 attggcttca gacctggaat caatgctaag gcttcgctgc gaagaaaaag gcattacgct
    12061 tacttttgaa acgattgaat tgcctaagag aatcatcacc gaccctagcc gtctaaagca
    12121 aattctgatc aacatgatcg gtaatgcgat taaatttacc agtcatggcg gagtgaaggt
    12181 aacccttttc acgcaagctc agaaattaat ctgtacagtg gaagacacgg gtgtcggtat
    12241 tcctgttgaa agcaggcacc gattgtttca acccttctca caggtggacc tctccatccg
    12301 taagcaatat ggcggcacgg gtctggggtt gatcctctcc cgccgactcg ccaaactcat
    12361 gggtggtgat gtgattctaa aatatagtga gcctggtcgc ggcagtgtat ttgaggtctc
    12421 gattgctttt gaagccgata acacagttga agtcgcctct aaacgtcgtg aactgccagc
    12481 agagacctcg ttaagtctca atggaaaacg aattttgatc gttgaggact cgttggacaa
    12541 ccaactctta gcacagcagt atatcctcaa ggaaggcgcc gaagttatta tcgccaacaa
    12601 cggccttgaa gcactgagag ccatgacttt gcacaccttc gatctcgttt tgatggacat
    12661 gcaaatgccc gtgatggatg gatacacggc aaccttagag ctgcgagata aaggttttac
    12721 aatccccatc attgcattga cagccaacgc gatgaaagag gatcttgaac gttgtctgca
    12781 ttccggctgc aacgattatt tatgtaagcc ctttcgccgc ggggacttat taaacatgat
    12841 agctcagcat tgtcagaacc ctaaaaacca gactgtgtct tagatcccaa gaaccgaggg
    12901 ctcagcctcg gttccaggat ttattgagga acctttggaa tcaatttttt cttcaattgt
    12961 gtccatcact tttaattcat cactagcatc tctaatcatt tttgtttgat atgcagaagt
    13021 ttcttgtaat tgatttcgag atcagatgtt ttctactttg ctagtttttc gtccccagag
    13081 aaagtctcca agatacgtag tcatctagac tgcgagataa tgcgaaccaa tccatatgca
    13141 aaaattcatt acgcaattta aggcccttag agatttgcag cgaaagcgac aataaatctc
    13201 ctagtgaagc ggccccgcga gctgtgggat tggctacaaa gaaccaccac tcgaattcgg
    13261 caataagtct ctgtgttgtt gtgatatcac tggatttgag tgtcatatcc catagcttac
    13321 ttaaatgatt tcgaatcgcg ggaacatcct gattttgagc atgtttgacc caatagattc
    13381 catcaaagac aatctcaaga gctggtgcat ttaaattagg accatttgct ggcatgggta
    13441 aaacgccttt agtgcaccaa tctgttcaag tgtcaaacct aagatcaatt gttccatttg
    13501 ttctcccatc atgcttgtcc ccatcctgtt ttggtatgga cgcggctttt tattacttgg
    13561 agttcctttg cttgcttttc agttcgattt cgagaccaga gatacaatcg agaaaggtag
    13621 acatagacga cacgaaggcg aataaaaaga tcaatcaatt cttcataatg ctctggtaaa
    13681 ctataaactg acgagtatcc ttgcaaaata ttttctcgga tctcatcgcg gttcttttta
    13741 cgccgaactg tcgaaagcga aatggcgagg tctgaaagaa accaatggta gcagcaattg
    13801 ccaaaatcga acgccgtaag ccgtttaccg gtggggtcat atcggaaatt ttgcggagca
    13861 tggtcggcat ggatcagacc aaattcagag ggcctttttt gaagctgctg gcaagaagta
    13921 ataacttcgt taaattcctc gcgtgctatt ttatcttcgg atgggattaa gtcatccgct
    13981 ctggaaatga ggatttcatt atcccattgc catctctttg gttcctcatt tttgataaca
    14041 tatttacttg cagcttgatg aatgagtcca agattacgcc cccattcttt aaaaaattct
    14101 gagttccaca attcagcccc ttcctggaca tcgacgcctg gcgcataggc aatcgaagaa
    14161 caaatgagac tgcctgtcga gcactcagcc caaaatacaa gctcgccgtc tttggtggaa
    14221 agaccttggg ctacggggat attcgctcca tacagatgat tcacaaagtt tagctcggcg
    14281 gtcacctcat caaacgacct aaattcgttg tcggtgaatc gaagaatttg taattttcca
    14341 tctgagtttt tgaacgaaaa tacggaatta ccactgtcgc cgagatgttc aatggaagaa
    14401 ggatctccac cccatctact gacaaatttt cttgcatctt ctaaatttgc aaagcgaatc
    14461 atgaatcact tgccctgttg cctatttttt tcataagctc ggaaattttc ctgctatctt
    14521 catccgttcg ctcggactcc agcccggcga tggctccatt tcgatcagaa agggatcgat
    14581 tctggctaag cttgaactta gcattgatgt ctttaacctc aatttcaaac ccagtgatag
    14641 cggaagttag atcagccgga gactcaagat cagttggtag ccaaaaattc catgggttct
    14701 tagacttagc ctccacttgg gccgtcagat ttttcaggca ggcaacaatt ccgtcatagt
    14761 cagaaatcaa tctaccttgt cctacggcgt ggacaactgc ataattccat gtcggcacgt
    14821 cattttctgc ataccagttc ggggttatgt aagcattcgg tccgtggaaa attgcggtga
    14881 ccggcagacc gtcaatcagt ttccagtggg gattggccct ggcaagatgg cc
//
LOCUS       DATBTE010000312         7246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1476854, whole genome shotgun sequence.
ACCESSION   DATBTE010000312 DATBTE010000000
VERSION     DATBTE010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476854"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="VIG33_11670"
     CDS             complement(<1..1391)
                     /locus_tag="VIG33_11670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8271537.1"
                     /translation="MSLRDQLKKLNVGMSRKAVSLSPHDANWFEAFSAVDAALNDCVP
                     KDLELHHIGSTSIPGIHAKPILDILGVVPSIEAFDNNRSELESLGFVWKGEYGIAGRR
                     YCVLYDESEEVGLVHLHVFAKSDREVEKHLIFRDYLKATAEASYRYEELKKKLAQSFM
                     DARTNYSEGKSELIAQLLDEAFKWKNNSIDLTPAMNLHSLAYQTDLSILALQGRLENR
                     EEYVVAESFGNPGYFWGNLLVMKNAPSQGDYARWIGLFKQEFRHQPLVKHMTFGWDST
                     DGFEGATEPFVEDGFEIERSVVLTLQQVRLINPKHSCVGLEIRPLVNDKDWEAAVQNQ
                     VDCRLQTFKLETFLPFKRIQMKKYRDMSEAGFGHWFGAFLNGKLIGDCGLYVFDGTGR
                     YQAVGTHPDFRKKGVCANLVFETARFGFEKLNAKMLVMVADPEYHAAKVYESVGFTPT
                     EKAIGVYRYPKDQ"
     gene            1526..2017
                     /locus_tag="VIG33_11675"
     CDS             1526..2017
                     /locus_tag="VIG33_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271538.1"
                     /translation="MVEAAGIGTNSFPNRATYLDSIEFFQKCHWKNTANYSALYLEKG
                     LSTAQIGEQLGIPKQTVLNQLKREGIRLGSNKGRLLANPENYRLAYAPYGFSKKDGRL
                     IPNKAELKTCRLVVQLRNNSKMSFQEIARVLVRRRIKNREDSISWSHATVGRIYKRWN
                     GKL"
     gene            2033..2221
                     /locus_tag="VIG33_11680"
     CDS             2033..2221
                     /locus_tag="VIG33_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271539.1"
                     /translation="MLAAIKDLITAIAATTILTYSTGQQEWLWKHIVALQHKALINAQ
                     KNLGCPSIFDKDSCKRIR"
     gene            complement(2271..2801)
                     /locus_tag="VIG33_11685"
     CDS             complement(2271..2801)
                     /locus_tag="VIG33_11685"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEY8271540.1"
                     /translation="MVTIVKGDIFKSPAQALTNAVNCVGVMGKGVALEFKNRYPQMFS
                     DYKLKCDQGKVKPGQPYLWEDDSVQILNFPTKRHWRDGSLLEDVEEGLKYLASSYVQM
                     GLQSIAMPALGCGLGGLKWSEVQSLIVKHLGSIPDLDVYVYEPQGAVAIRSDVDGSVS
                     DIQADLDKVSARPREF"
     gene            complement(2807..3451)
                     /locus_tag="VIG33_11690"
     CDS             complement(2807..3451)
                     /locus_tag="VIG33_11690"
                     /inference="COORDINATES: protein motif:HMM:NF025840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="HEY8271541.1"
                     /translation="MSQQINKKYQVWVAHHMTHIDNLRSILQSGELRSYNSMSGQGYR
                     NLANEDVQAGRSAIIVPVSQKPLHDYVPLYLGFKTPMVAVNQDYNEELLFLRFSLDIL
                     AMPGSIVCDGNARSQVSKFYLFSGPDVFSKVDVGAVRSVKYAKDPELRRKKQAEVLIP
                     DRLPISQMLDMICYSKSTQTRTLSILNEFGIKKPVNVNQGWYFLSSPQIGSKEG"
     gene            3759..6215
                     /locus_tag="VIG33_11695"
     CDS             3759..6215
                     /locus_tag="VIG33_11695"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEY8271542.1"
                     /translation="MGVDDQIRSAENRLAALEKERIDLLGELRNLRAQRDEQKPMLLL
                     GRPPLMKTPETNEEKADLFLTLFRGRENVYPKRWENLKTGRSGYSPVCENEWVKPICQ
                     KPTIKCSDCSHQKFSPLSALAIGAHLRGATTIGTYAIREDDTCPFLACDFDESSWQTD
                     LLAFCETAKSFGIDVAMERSRSGNGGHAWIFFAEPVPARLARSLGTLIIAKCSERNIR
                     LSLESYDRFFPTQDFLPKGGFGNLIALPLQKIPRENGNSCFVDESFVAISDQWAYLSS
                     VRRLYKYEVDLLLKEYLPKGNFKRADAFEDVAWLTDNEILDKTSIAQEHDRSLEGKNV
                     EITFGPMLSIPVEGLPTKVIARLKKTSSFANPEFYKLQRIRLQTYPNPRFIFSGEMRP
                     DHLLLPRGVLDKVTKILQEVGASVVIRDERIAKKKITVDFEGTLTPIQEKAVNKITES
                     DLGILMAPPGAGKTVMACKLIAERKVSTLILVHRQPLLEQWKERISSFLKIPVKEIGT
                     LSGAKKKMTGKIDVGMLQSISRLEDLSVIAEKYSQIIIDECHHVPATSFEAILKQLHA
                     RFVLGLSATPYRKDGLEKIMFLQCGPIRHEIQPAEVSALPKEVSIYETSLVFPDELGR
                     QPPYHVLIHHLVQNESRNHLITTKVAEVLEQNRFPLLVSDRKDHLDLLEQLIKKMSPD
                     VELVVLEGTLTNKQRRGALARIAELRFTKMKVMLLATASLIGEGFDLQALDTLIFATP
                     LSFEGRLIQYAGRIHRESETKKSAQIIDFVDSYSGMFLKMYRSRVSTYRKMGYRIHED
                     ERLIGPLALYSRSRPPLLSP"
     gene            complement(6244..>7246)
                     /locus_tag="VIG33_11700"
     CDS             complement(6244..>7246)
                     /locus_tag="VIG33_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271543.1"
                     /translation="GMSIHDAQVRVKQLNSQTIIRDQEKRIQENRRQEKIEQGKNEFC
                     LPVEFVAEFEKRFLRVRDSDTTNGRKKRSRAHIIWRAAQKAIAAIHMEPSEWFYHTHE
                     IYDHFHRQRYSVRYIHVILKFMNLWGFFICRKLALPFLGIPCPKGYERARLIEANYEK
                     QDATVRRPSLPLSPEDLSKVAAKLNQPNLNWLYLSIWLGLRPQEIDNLHNKEMWRLEK
                     NIAGKDILWVFQTKIVALPIEDRWKPIPIIFDEQKFAIRIIESNGFKRPITKTMRKYF
                     GPGTTLYAGRKGFTDLMISKGQILENISVWMGHSTLDRTWRSYKQKRKFHLRYSLES"
BASE COUNT         2079 a         1703 c         1491 g         1973 t
ORIGIN      
        1 cactgatctt tcggatatcg atacactccg atggctttct cggtaggtgt gaatccgacc
       61 gactcgtaaa ctttggcggc atgatattca ggatcggcaa ccatcactaa catcttcgca
      121 tttaattttt caaatccgaa tctggcagtt tcgaaaacca aattggcgca aacgcccttt
      181 tttcggaaat cgggatgagt acccaccgct tggtagcgac cagtaccatc gaaaacatat
      241 agaccacagt cgccgatgag ttttccattt aaaaaagcac caaaccaatg tccaaaccct
      301 gcttctgaca tatctcgata tttcttcatc tgaatacgct tgaagggaag aaatgtttcc
      361 aacttaaatg tttgaagtct gcaatcgacc tgattttgaa cagctgcttc ccagtcttta
      421 tcgttaacta acggacggat ctctaaccca acacatgaat gcttcgggtt tatcaatcga
      481 acttgctgta aagttaaaac aactgacctt tcgatttcaa atccatcttc gacaaaaggt
      541 tcagtagcgc cctcgaaccc atccgtagag tcccaaccga acgtcatatg ttttactaat
      601 ggttgatgcc taaactcttg tttgaacaag ccaatccatc ttgcgtagtc gccctgcgaa
      661 ggagcgtttt tcataacgag aagatttccc cagaaatatc caggattacc aaacgactcc
      721 gcgacgacat actcttctcg attttccaaa cggccttgca aggccaaaat cgaaaggtca
      781 gtttggtagg cgagagaatg aagattcatg gccggagtca aatcaataga attgtttttc
      841 cacttaaaag cttcgtccaa caattgagcg attagttctg acttgccctc agagtagttc
      901 gtacgtgcat ccataaaaga ttgagccagc ttttttttaa gttcctcata gcgataagac
      961 gcctctgctg tagcctttaa atagtcccga aaaataagat gcttttctac ttcacgatct
     1021 gattttgcaa agacatgaag atggaccaac ccgacctctt cactctcatc gtaaagaacg
     1081 cagtatcgcc taccggcaat tccgtattcg cctttccaaa caaaccccaa agattccaat
     1141 tctgatcgat tgttatcaaa agcttcgatt gatggaacga cacctagaat gtccaaaatt
     1201 ggttttgcat ggattccggg gatggaagtg ctaccgatgt gatgaagttc gagatcctta
     1261 ggaacacagt cattaagagc ggcatccact gcactgaatg cctcaaacca atttgcatcg
     1321 tgcggagata gagaaacagc cttacgactc attcctacat tcaatttttt taattggtca
     1381 cgtaatgaca tttcgccaat cccctaaatc aaaacatcct tgtaagctat tgattccctg
     1441 tagaacctcg gaacctgagg ttgattcccc cagaggggag aattcaatat ttctaacaac
     1501 ttagaaccta ttccaggttc cgtatatggt ggaggcggcg ggtatcggaa cgaactcttt
     1561 tccgaatcgc gctacctatt tagactctat agagtttttt cagaagtgcc actggaaaaa
     1621 cactgcgaac tatagtgcgc tttacctaga aaagggcctt tctactgcac aaatcggtga
     1681 gcaactcggc attccaaaac agacggtcct caatcaactc aagcgcgaag gcatccgcct
     1741 tggcagtaat aaggggcggc tcctcgccaa tcctgagaac taccgtctcg cctatgcccc
     1801 ttacgggttt tcaaagaaag atggacgcct catccccaac aaagccgaac tgaaaacttg
     1861 ccgactcgtc gtccagctcc ggaacaactc gaaaatgtcc tttcaagaaa tcgcacgagt
     1921 gcttgtgcgt cgtcgaatca aaaacaggga agattccatt tcctggagcc acgcgaccgt
     1981 cggtcgcatc tataaacgat ggaatggaaa actctaaccc aaggagcaaa ctatgcttgc
     2041 agcaatcaag gatctcatca ccgcaatcgc cgctaccact attctgactt actcgactgg
     2101 tcagcaggaa tggctttgga aacatatcgt ggctcttcaa cacaaagcac tgatcaatgc
     2161 tcagaagaac ttgggatgcc caagtatatt tgataaggac tcttgcaaga gaataagata
     2221 agtccgaact tagaccatcg cctaaagtga taaatcgctg actatgttgc ttaaaactct
     2281 cgtggccgag ctgatacttt atccaggtca gcctgaatat ccgagacact gccatcgaca
     2341 tcacttctaa tagcaacagc tccctgcggc tcataaacat aaacatcgag atcaggaatt
     2401 gaacccagat gcttcacgat cagcgactgc acctctgacc atttaagacc gcctagtcca
     2461 cagccgagag ctggcattgc tatggattgg agtcccattt gtacgtagga gctcgccaaa
     2521 tattttagcc cctcttcgac atcctcaagt agagaaccgt ctcgccaatg cctcttcgtg
     2581 gggaagttaa gaatctgaac agagtcgtct tcccaaagat acggttggcc tggttttact
     2641 ttcccctgat cacattttaa tttgtagtcg ctgaacattt gaggataacg attcttaaat
     2701 tcaagagcca cacctttgcc catgactcca acacagttca cagcgttcgt taaagcctgt
     2761 gctggagatt taaaaatgtc gccttttaca attgtgacca cttaaattat ccttctttgg
     2821 agccgatttg cggagagctt aggaagtacc agccctgatt gacattaacc ggcttcttta
     2881 taccaaattc attcaaaatg gacaaggtgc gagtttgggt actttttgag tagcaaatca
     2941 tatcgagcat ttgagatata gggagcctat caggaattaa aacctcagcc tgcttctttc
     3001 tcctcagctc tgggtccttg gcgtatttaa ccgatctgac ggcgccaaca tcaactttgg
     3061 agaaaacatc ggggccacta aataaataga atttagaaac ctgagaccta gcatttccgt
     3121 cacatactat ggatccaggc attgccaaaa tatccaatga aaatcgtaga aatagtaact
     3181 cttcgttgta atcctgattg accgcaacca tcggggtttt gaagccaaga tacagtggga
     3241 catagtcatg taaaggcttc tgagataccg gcacaataat agctgagcgt cctgcctgta
     3301 catcctcgtt cgcgagattc ctatagcctt gcccactcat tgaattataa gatcgtagct
     3361 ctccagattg gagaatcgat cgcagattat caatgtgggt catgtgatga gcgacccaca
     3421 cctggtactt tttattgatt tgctggctca acgatttgct caccgacacc cttttcgtct
     3481 aaagggtaaa cacctatcac atcgcagtcg ccgagaacac gcattttata actttgacca
     3541 cattccgttg ataggatatt taccattaat ccgaaaggcg gacggactat gtgcagacaa
     3601 gagcaagaac tggcgcaaat ggctgctatg gctcctggcc tatcccaaga ccgaatttca
     3661 aaaatattct caggacagat cggccacatg agtattgata agttggtaga aattcattct
     3721 gctctgaagc taaaggtctc aatacaaaca aggcgatcat gggcgtagac gatcaaatcc
     3781 gctcagctga aaatcgactg gcggctcttg aaaaagaacg catcgattta ctgggtgaat
     3841 taagaaatct gcgagcccaa agggacgaac agaagcctat gcttttattg ggccgcccac
     3901 ccctgatgaa aacccccgaa acaaatgaag agaaggctga tttatttttg actctatttc
     3961 gagggcgaga aaatgtatat cccaagcgct gggagaatct caaaaccggc agaagcggat
     4021 actcgccagt gtgtgaaaat gaatgggtaa agccgatttg ccagaagcct actatcaagt
     4081 gtagcgattg tagccatcag aaattctcac cgttgagtgc tttggcgatt ggagctcacc
     4141 ttcgcggagc aaccacaatt ggcacctatg ccattcgaga ggatgacact tgtccatttc
     4201 tagcttgtga ttttgacgaa tcttcttggc aaactgacct tttagcattt tgtgaaacag
     4261 cgaagtcctt tggtatcgac gtggcaatgg agagatcacg ttctggtaac ggcggtcatg
     4321 cttggatatt ctttgcagaa cctgtgcccg cacgactggc aagatcgctc ggaactctta
     4381 tcattgcaaa atgttcggag cgcaatatcc gactgagtct tgagtcctat gatcggttct
     4441 tccccactca ggattttttg ccgaaagggg gatttgggaa tttaattgca ttgccgttac
     4501 agaaaatacc tagagaaaac ggcaacagtt gttttgtaga tgaatccttt gtggctattt
     4561 cggatcaatg ggcgtatcta tcatcagttc ggcggctcta caaatatgaa gtcgatttat
     4621 tactaaaaga atacctgcca aaggggaatt tcaaaagagc tgatgccttt gaagatgttg
     4681 cctggctcac cgacaacgaa attcttgata aaacctcgat cgcccaagag catgaccgct
     4741 ccctcgaagg taagaatgtt gaaattacat ttggtccaat gctatcaatt ccagttgagg
     4801 gcttgccgac aaaagtaatt gcccgcctta aaaaaacttc tagctttgcc aatcctgagt
     4861 tttacaaact ccaacggata aggttgcaaa catatccaaa tccgagattt attttttctg
     4921 gagaaatgcg gccagatcat cttcttttac ctagaggcgt cttggataaa gtcacaaaaa
     4981 tcctgcaaga agtaggagcc agcgttgtta ttcgcgatga gcggatagcc aaaaagaaaa
     5041 taacggtcga ttttgagggc accctgactc caatacagga aaaggccgtg aataagatca
     5101 cggaatctga tttgggcatt ctgatggccc cacctggggc tggtaaaact gtcatggcgt
     5161 gtaaactcat tgccgagaga aaagtttcaa cattgattct tgttcacaga cagcctttgc
     5221 tagagcaatg gaaggaacgc atttcaagtt ttttaaaaat acctgttaaa gaaattggca
     5281 ctttgagtgg agcaaaaaag aaaatgaccg gtaaaatcga tgtcgggatg ctccagtcaa
     5341 taagtcgcct agaggacctt tctgtaatcg ccgaaaaata ttctcaaatc atcatcgacg
     5401 agtgtcatca cgttccggcc acttcatttg aagccattct gaaacaatta cacgcgagat
     5461 ttgttctcgg cttgtcagcc acgccatacc gaaaagatgg acttgagaag atcatgtttt
     5521 tacagtgcgg tcccattcgc cacgaaatac aaccagcaga ggtttcagcg cttccaaaag
     5581 aagtctctat ttatgagacc tctctcgtat tccctgatga attgggacga caaccgccct
     5641 atcacgtttt gattcaccac cttgtacaaa atgaatcacg caatcatctt atcacgacaa
     5701 aagttgcaga ggttcttgaa cagaatcgct tcccactttt agtttcggat cgcaaagatc
     5761 atcttgatct tctagagcag ttaatcaaaa aaatgagccc cgatgttgaa cttgttgtgc
     5821 ttgaaggaac tctcacgaat aaacaacgcc gtggagctct agctcgaatt gccgagcttc
     5881 gattcaccaa aatgaaagtg atgttgctag caacggcatc tttaatcggc gaagggttcg
     5941 acttgcaggc gttggacaca cttatttttg caacgccact ttcatttgaa ggcagactca
     6001 ttcagtacgc tggacgaatt cacagggagt ctgagaccaa gaagtcagct caaattattg
     6061 attttgtgga ctcctatagc gggatgtttc tcaagatgta tcgcagccgc gtttcaactt
     6121 atagaaagat gggttatcgt atccacgaag atgaacgcct cattggtccc ctggctttgt
     6181 atagccggtc aagacctcca ctgctgagtc cataacatcc atcaaaccca ccacatgatt
     6241 ttatcaagat tccaacgaat aacgtagatg gaattttctc ttttgcttat agctcctcca
     6301 agttctgtcc aaagtcgaat gtcccatcca gacactaata ttctccaaaa tctgaccttt
     6361 tgaaatcatc aaatcagtaa atccttttcg tcctgcataa agcgtggtcc ctggtccaaa
     6421 atattttcgc attgtctttg tgatcggtcg cttaaaacca tttgattcta tgattcgaat
     6481 cgcgaatttt tgctcatcaa aaattatcgg aattggtttc cagcgatcct caatgggaag
     6541 tgctacaatt tttgtttgaa atacccacaa aatgtccttg cctgcaatat tcttctcaag
     6601 tctccacatt tctttattat gtaagttatc aatctcctgg ggtctcaaac ccagccaaat
     6661 tgacagatac agccagttta gattcggttg gttgagctta gctgcaactt ttgaaagatc
     6721 ctctggcgac aatggcaatg aaggtcttct gactgtcgca tcttgctttt cataattagc
     6781 ctcaatgagt cttgcccgtt catatccttt aggacaagga ataccaagaa atggaagcgc
     6841 aagcttcctg caaataaaaa agccccagag attcataaat ttcaaaataa cgtggatata
     6901 tctaacactg tagcgttgcc tgtgaaagtg atcataaatc tcatgagtat gataaaacca
     6961 ctcgctgggt tccatatgaa ttgcggcgat agccttctgg gcggctcgcc agattatgtg
     7021 agcacgagat ctcttttttc taccatttgt agtgtcacta tctcgaactc gcagaaatcg
     7081 tttttcaaat tcagcgacaa actccactgg taagcaaaat tcatttttcc cttgctcaat
     7141 tttttcttga cggcggttct cttgtattcg cttttcctgg tcccgaatga ttgtctggga
     7201 atttagctgt tttactcgca cctgagcatc atgaatagac attcct
//
LOCUS       DATBTE010000313         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_110743, whole genome shotgun sequence.
ACCESSION   DATBTE010000313 DATBTE010000000
VERSION     DATBTE010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110743"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VIG33_11705"
     CDS             complement(<1..708)
                     /locus_tag="VIG33_11705"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8271544.1"
                     /translation="MSDLTKWQLHEWKPKTIKFDWTPKEKNLNQASGVCALVDLLVAS
                     PFYDELKKCLPERLSNNSYHPLHFAILLIAGFWMGYDCLDDLEEFSNNPAIKAQFGKI
                     PLAKTFGDYLRDFSGENILALRTLLTRQALYYREALKKDSSITFDMDSTDHEHHGDLI
                     EGLEFNYKGKWCLDSLEVFDDLGFCYDFDLRPGSTFSSVGSVDMMEKILLHHTLRVPL
                     KDDFARLDSAFCTEEMIR"
     gene            1405..2043
                     /locus_tag="VIG33_11710"
     CDS             1405..2043
                     /locus_tag="VIG33_11710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEY8271545.1"
                     /translation="MTEKVKPLLRGHFHQAGFFIALGASSVLIMIAQGLYAKLVCLAY
                     AFSLCGMFAISGLYHRINWTPKARAFWRHLDHSAIFLLIAGTGTPICLLGMRSESGLR
                     LFWVFWSMAIAGIFKELLWIQAPKWLSAGFYVIMGWAGIPYLSEMSKALGGMNISFMI
                     AGGIIYTIGAVIYAIKKPNPFPKAFGYHEIFHILIMIAAIFHFIVIYELVTR"
BASE COUNT          597 a          412 c          410 g          646 t
ORIGIN      
        1 ccgaatcatt tcttcggtac aaaacgcgga atccagtctt gcaaagtcat cttttagggg
       61 aactcgtaac gtgtggtgaa gcaagatctt ttccatcatg tccactgagc ccacggaaga
      121 aaatgtggat ccaggacgca ggtcaaaatc ataacaaaac ccgagatcat caaacacctc
      181 aagtgagtcc aaacaccact tccctttata gttaaactcc aagccctcaa taagatctcc
      241 gtgatgctcg tgatccgttg agtccatatc aaaagtgatg gaagaatctt tttttaaagc
      301 ctcgcggtag taaagggcct gacgggttaa gagtgttctt agtgctaaaa tattttcccc
      361 tgaaaaatcc ctgagataat cgccaaaggt ttttgctaac gggatttttc caaactgtgc
      421 tttaatagcg ggattatttg aaaactcctc tagatcatca aggcaatcat aacccatcca
      481 aaatcctgcg attaaaagaa tcgcaaagtg aagtggatga taggaattgt tgctgagcct
      541 ttccggaaga catttcttta gttcatcata aaaaggggat gccaccaata gatccaccaa
      601 ggcacatacg cctgaggcct gatttaaatt tttctctttc ggggtccaat caaactttat
      661 tgtttttggc ttccactcat ggagttgcca ctttgtgagg tctgacataa gatccttcct
      721 tgtctgcaaa atttagcttt tttgccggtt gggatcttta gttttattgt cgtaggtcca
      781 ctgagtgaaa gcgttaacgc ttaaaaaata acagtcaaat cacagaaatc actaaattac
      841 tggtgagttt attgattctt taaaatcgga tttgggtaca aatttaaata taggccccgt
      901 tggctatgta gccccttcat tacctaagtg taccattagg tctaaggatg aatacccagg
      961 cttccctaaa gagtgagtct cagatttgaa tttgggacct acagcattgc attttaactt
     1021 gattttcatc tagttagtaa aaacatcaag cagttttctt cctccctttc acttgttagg
     1081 cttatggaaa cttcattcag gggatgccat gagttttata cgaattgttt ctcgagacga
     1141 ataggctgat ttaacattaa aatcagctat tttaattaaa atcaaaattc tatcctggaa
     1201 agacccctca attgaagaag ccctcaaagc catttctaat cacggagtta tctgcccatt
     1261 aattgaactg tggagacttc gccatcaaaa gtaatctaaa ttgagacgtt atccattaca
     1321 gaaaatatga cagatttaat ttgaaattcg acaaatagga atttcttaat gtgacagcat
     1381 ctgcattcaa agttatcgtt attcatgact gaaaaagtga aaccccttct tcgcggacac
     1441 tttcatcaag ctggattttt cattgcccta ggggcgagca gcgtcttaat tatgatagct
     1501 caaggccttt atgctaaatt agtctgcttg gcctatgcat ttagtctttg cggaatgttt
     1561 gcaataagtg gcctttatca ccgtatcaat tggacaccaa aagctcgggc tttttggcgc
     1621 caccttgatc attcagctat ttttcttttg attgcgggca cagggactcc gatttgcctg
     1681 ttgggaatga ggagtgagtc aggtttacgt cttttttggg ttttttggag catggcaatt
     1741 gcaggaattt tcaaggagtt gctctggatc caagctccaa aatggctgtc ggcaggcttt
     1801 tatgtgatta tggggtgggc aggcattcct tacctctcag aaatgagtaa ggctttagga
     1861 ggcatgaata taagcttcat gattgctgga ggaatcattt atacaatcgg cgcagtgatt
     1921 tatgcgataa aaaaaccaaa tccttttcca aaggcatttg gctatcatga aattttccat
     1981 attctcataa tgatagccgc catattccat tttatcgtga tatatgaact tgtaacaaga
     2041 tagtgaataa cttcatttca ctctt
//
LOCUS       DATBTE010000314         1683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2296712, whole genome shotgun sequence.
ACCESSION   DATBTE010000314 DATBTE010000000
VERSION     DATBTE010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2296712"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..385
                     /locus_tag="VIG33_11715"
     CDS             203..385
                     /locus_tag="VIG33_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271546.1"
                     /translation="MGLDNVIKLALTLTLAAAMTGQLPRITNQIRHAQVKFLEQSKAS
                     QWGSPGLLSSQRKDRR"
     gene            491..1090
                     /locus_tag="VIG33_11720"
     CDS             491..1090
                     /locus_tag="VIG33_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8271547.1"
                     /translation="MERITRKEQKERTRANLVDEALHLFSTKGISSTTTADVAKALDV
                     SHGTVFVHFPTREELILAAVERFGERLSVRLNAKLTSDLHLKEILQAHLSVLTEFEDF
                     YLRVICESQTLPPQIRSLVYSMNAAISYRLFHAAQKQMKDGSIKRIPQASLFNSWMAL
                     LNYHIMNRDLLSEKSPILKHKGDEILRLFLMLIKTREDK"
     gene            1087..1344
                     /locus_tag="VIG33_11725"
     CDS             1087..1344
                     /locus_tag="VIG33_11725"
                     /inference="COORDINATES: protein motif:HMM:NF024086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEY8271548.1"
                     /translation="MKPKCKSCSSCGMPMENPKDFSLGDPSSVYCRYCTDNSGKLLPY
                     EKILKANADYFKESQGLTSEAAHKMATELLKSQPAWKNLGV"
BASE COUNT          509 a          398 c          367 g          409 t
ORIGIN      
        1 ctgaataccc aaaaactccg gcctcgctgt gcctcttcat ggactcggtt taccgtccgc
       61 caaatcatca agtggcatca ggtaaacccg gatgtatttc tgaagcgcga tggcaagatc
      121 gtgacctacc agccttggga gtctactaat tcttctgaaa gtgaaacgat cgacaaatcc
      181 aagcccaaaa ggagcaaaca tcatgggact cgataacgtc attaaactcg cactcactct
      241 gactctggct gctgcaatga ctggccagtt accccgcatc accaaccaaa tccgacacgc
      301 acaggtgaaa ttcctggagc agtccaaagc aagccagtgg ggttcgccgg gcctgctgtc
      361 ctcccagcgc aaggatcgtc gatagaaaat cagccagttc gacatttcag atatatcctc
      421 cgataggttg tttaatgttt aacacttttc agttgactaa gctcttactc actgagtatg
      481 tacttatcca atggaacgaa ttacccgaaa agaacaaaaa gaacgtacgc gagcgaacct
      541 tgttgatgag gctcttcatt tgttttcaac gaagggtatc tcgtccacaa cgacggcaga
      601 tgtggccaag gccctcgacg tttcccatgg gacagttttt gttcacttcc caactcgtga
      661 ggagttgata cttgctgctg ttgagaggtt tggagaacgc ttgtcggtaa ggctgaatgc
      721 aaaattgacc agcgacctgc atcttaagga aatacttcag gcccacctga gtgtcctgac
      781 cgagttcgag gatttttact tacgggtaat ctgcgagtct cagacacttc cacctcaaat
      841 cagaagcctc gtttattcga tgaatgccgc tatttcgtat cggttgtttc atgccgctca
      901 gaaacaaatg aaagatggtt ctataaaaag gattccacag gcctccttat tcaattcatg
      961 gatggctcta ctgaattatc acattatgaa tcgcgatctt ctttctgaga aatcgccgat
     1021 tcttaaacac aaaggagacg agatacttcg tctttttctt atgctcatta aaacaaggga
     1081 ggataaatga aaccaaaatg taaatcgtgc agctcatgtg ggatgcccat ggaaaatccg
     1141 aaagactttt ccttaggaga cccatcaagt gtttactgtc gatactgcac agataactcg
     1201 ggaaaacttc tgccctatga aaaaatcctc aaagcgaatg ctgactactt caaggaatcg
     1261 cagggactga caagcgaagc agcacacaaa atggccacag agctcttaaa aagccaacct
     1321 gcttggaaga atctcggagt atagcgatgg caacggaaaa aaagaagaag acctattgtg
     1381 ttgtttgccc gacctgggaa attaaaaacc cggaccgctg ttcaaatact ctcaaaaaag
     1441 actcaagagc actctatttc tgcaccaaga aatgtaagga gagatacttg aagacaccgg
     1501 aaaagttcaa atgaatatct ccctaaaacc actaactgca gaaaattttg cagactacga
     1561 atcgctcacg agctgccaaa gcggtggtgg atgcttttgc tcattttggc atcaaaaatg
     1621 gaattcaatg gccgattggg agctggccaa aaaggaagct ccagagatca atcgaagcat
     1681 ggt
//
LOCUS       DATBTE010000315         4371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2158909, whole genome shotgun sequence.
ACCESSION   DATBTE010000315 DATBTE010000000
VERSION     DATBTE010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2158909"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..358
                     /gene="rpsL"
                     /locus_tag="VIG33_11730"
     CDS             5..358
                     /gene="rpsL"
                     /locus_tag="VIG33_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEY8271549.1"
                     /translation="MKSERIVQKNQTKSPALTQCPQRRGVCTRVYTTTPKKPNSALRK
                     VARVRLSNGYEVTSYIPGVGHNLQEHSVVLIRGGRVKDLPGVRYHIIRGVLDLQGVNG
                     RLRARSKYGTKRPKK"
     gene            378..851
                     /gene="rpsG"
                     /locus_tag="VIG33_11735"
     CDS             378..851
                     /gene="rpsG"
                     /locus_tag="VIG33_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEY8271550.1"
                     /translation="MSRRKKTFKREIIPDPVFKDLVVAKFVNKMMIQGKKGIAQALFY
                     GALEELKGKVPNEEPIAILKKALENAKPSIEVRSRRVGGATYQVPVDVRPSRRLALAM
                     RWLIAYSRERGEKDFSKRLAGELMDAFNNRGNSIKKKDDVHRMAEANKAFSHYNW"
     gene            861..2966
                     /gene="fusA"
                     /locus_tag="VIG33_11740"
     CDS             861..2966
                     /gene="fusA"
                     /locus_tag="VIG33_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY8271551.1"
                     /translation="MSAKDPKVIADLKYTRNIGIMAHIDAGKTTTTERILYYTGKSHK
                     IGEVHDGAATMDWMVQEQERGITITSAATMCFWKDHRVNIIDTPGHIDFTIEVERSLR
                     VLDGAITVFDGVNGVEPQSETVWRQADKYHVPRICFINKMDRVGADFVMSLNSIKEKL
                     GANPIAVQVPVGIEDTFRGVVDLLENKTYIWDTGGKDEHFRTIDVPEDMRAEVDKYRL
                     EVIEKIVEFDDVLLEKYLNGEEVTVPELKAALRKGTLALKAFPTFCGAAFKNKGVQPL
                     LDGVIDYLPSPLDVPSIEGYDPEKPDKKILCRTDFNAHTAALAFKIANDPFAGTLTYI
                     RVYSGETKVGDQLLNPRTEKKERIQKLVKLHANSREEVLSLKAGDIGAVVGLKFTGTG
                     DTLCEASHPVVLETITFPEPVISVAIEAKSSADQEKMLAGLAKLEKEDPSCKLRSDPE
                     TGQILLSGMGELHLEILVDRLLREYKIQANIGKPQVSYRETITSSATVDYVYERQLGG
                     EEHFAKIKLIIEPIPLVNGLQFVTKVTPTSLIQPGYIKAVENGFKESAEVGPLASYSM
                     IGIRGTLMEAEARQDSSNEIAFKAAASLAFREAVKKVTTELLEPMFKLEVACPDEFVG
                     NIVGDLNSRRGKIISMNVRPGGGQIISAEAPLATLFGYATDIRSLSQGRASFSMEFLV
                     YAVVPLKVKSEILTKLGRF"
     gene            3178..3495
                     /gene="rpsJ"
                     /locus_tag="VIG33_11745"
     CDS             3178..3495
                     /gene="rpsJ"
                     /locus_tag="VIG33_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEY8271552.1"
                     /translation="MQSQKIRIRLKAFDHKLLDQSTKEIVETARRTGAKIAGPIPLPT
                     RINRYTVLRSPHVDKKSREQFEVRTHKRMLDILEPTQQTVDQLMKLDLSAGVDVEIKL
                     SAI"
     gene            3508..4197
                     /gene="rplC"
                     /locus_tag="VIG33_11750"
     CDS             3508..4197
                     /gene="rplC"
                     /locus_tag="VIG33_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEY8271553.1"
                     /translation="MSEATNAGAEALTNNTSSEQGLKLNGLFAFKEGMSTIFNSQGEA
                     VPVTVLRYEPWVISQIKTNDKDGYEAIQVACHPKKAKNSNKSEKGHLAKAGFENGAQF
                     VKELRQALPEGASLGAQVSIESLVKGDFVKITSKSKGKGFAGSVKRWNFAGGPASHGS
                     HFHRRPGSSGNRTWPGRVMPGKKYPGQLGSETITVRNVEIVEINATENVVLVKGPVPG
                     ARNTLVKLVRE"
     gene            4200..>4371
                     /gene="rplD"
                     /locus_tag="VIG33_11755"
     CDS             4200..>4371
                     /gene="rplD"
                     /locus_tag="VIG33_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY8271554.1"
                     /translation="MAVVNVLNWKKEKVGQVDLAANVFEAPVKKDILHNVVQWQLASR
                     RQGTHMTKTKGLV"
BASE COUNT         1278 a          790 c         1042 g         1261 t
ORIGIN      
        1 gctcattaaa agtgagcgta ttgttcaaaa aaatcaaact aagtcaccag ctctgactca
       61 gtgtcctcag cgccgtggtg tttgcactcg tgtgtatacg acgactccga agaagccgaa
      121 ttcggctttg agaaaagtgg cacgtgttag attgtcaaat ggttacgaag tgacatctta
      181 cattcctggg gtaggtcata acttacaaga acatagtgtt gttttaatcc gcggtggtcg
      241 tgtaaaagac ttaccagggg ttcgctatca catcattcgt ggtgtattgg atcttcaagg
      301 tgttaatgga cgtcttcgtg cgcgatctaa atacggcact aaaagaccta agaaatagtc
      361 attaagaggt tataaaaatg tcacgtcgta aaaagacctt taaaagagaa attattcctg
      421 atccagtttt caaagatctc gttgttgcga agttcgttaa caaaatgatg atccaaggaa
      481 aaaaaggtat cgctcaagca cttttctatg gagctcttga ggagcttaaa ggaaaagtac
      541 ctaatgagga acctattgct atcttgaaaa aagcattgga aaacgcaaaa ccttccattg
      601 aagttcgttc tcgccgcgtt ggtggagcta cttatcaagt tcccgtagat gtacgccctt
      661 ctcgtcgttt agctttggct atgagatggt tgatcgctta ctcacgtgag cgtggtgaaa
      721 aggatttttc aaaacgcctt gctggtgagt tgatggacgc tttcaataac agaggaaact
      781 ctattaagaa gaaagacgat gttcatagaa tggctgaagc aaataaggca ttctcgcatt
      841 ataattggta atcttttcta atgtcagcga aagatccaaa agttatagct gatctaaagt
      901 acactcgtaa tatcggcatc atggctcaca tagatgccgg taagacgacc accaccgaac
      961 gaattcttta ttataccggc aaaagtcata aaattggaga ggtccatgat ggtgcagcta
     1021 ccatggattg gatggttcag gagcaggagc ggggtatcac cattacttct gcagcgacca
     1081 tgtgtttctg gaaagaccac cgtgtcaata ttatcgacac tcctggccat attgatttca
     1141 caattgaagt tgagcgttca ctccgagtcc tcgatggggc aattactgtt tttgacggcg
     1201 tgaatggagt agagcctcag tctgaaaccg tttggcggca ggctgataaa tatcacgtcc
     1261 ctcgaatctg tttcataaat aaaatggatc gcgttggggc cgattttgta atgagtttga
     1321 attccatcaa agaaaagctt ggcgccaacc ctatagctgt acaagttcca gtggggattg
     1381 aggatacgtt tagaggtgta gtagatcttt tggaaaacaa aacttatatc tgggatactg
     1441 gcggcaaaga cgaacatttt agaacaatag atgtgcctga ggatatgagg gctgaagtcg
     1501 ataaatatcg tcttgaagtc attgaaaaaa ttgttgaatt tgatgatgtc cttttggaaa
     1561 aatatttaaa tggtgaggaa gttactgttc ctgaattaaa agctgcactt agaaaaggaa
     1621 ctttggctct taaggcgttt cctacttttt gtggcgctgc ttttaaaaat aaaggcgtgc
     1681 aaccacttct tgatggagtg atcgattacc ttccttctcc cttagatgtg ccgtcaatcg
     1741 aagggtacga tccggaaaaa cctgataaaa aaattctttg taggactgac tttaatgcgc
     1801 acacagctgc tcttgctttt aagattgcca atgatccttt tgcgggaaca ctgacttaca
     1861 ttcgtgtcta ctctggtgaa actaaagtag gggatcagct tttgaaccca cgtacggaga
     1921 aaaaagagcg tattcagaag cttgtaaagc ttcatgcgaa ttctcgtgag gaagttcttt
     1981 ctcttaaagc aggagatatc ggtgctgtgg ttggtcttaa gtttaccgga acaggtgata
     2041 ctttatgtga ggcttctcat ccggtggtgt tggaaacaat tactttccca gaacctgtga
     2101 tctctgtggc tattgaagca aaatcttctg cggaccaaga gaaaatgttg gcgggcttgg
     2161 cgaagctaga aaaagaagat ccttcttgta aactgaggag tgatcctgag actggccaaa
     2221 ttcttctctc cggaatggga gagcttcatt tggaaatatt agtagatcgg cttttgcggg
     2281 aatataaaat tcaagctaat attggtaagc ctcaggtttc ttatagagaa actattacat
     2341 cttctgcgac tgtggactac gtttatgaac gtcagcttgg cggagaagag cattttgcaa
     2401 agataaaatt aataattgag ccaattccat tagtgaatgg tttgcagttt gttacaaagg
     2461 taacgcctac ttcgctaatt caaccagggt atattaaggc tgtggagaat ggttttaaag
     2521 agtctgctga agtaggacct cttgcgagct attcaatgat tggtattcgc gggactttga
     2581 tggaagcgga agctcggcag gattcatcga atgagattgc cttcaaggca gcagcttctt
     2641 tggcatttcg cgaagctgta aagaaggtga ccactgagct tttagagccc atgtttaagc
     2701 ttgaagttgc atgtccggat gagtttgtag gtaatattgt gggagattta aactctcgcc
     2761 gtggaaagat catttctatg aatgtacgac cgggtggtgg gcaaatcatc tcagcagagg
     2821 cgccgctggc gactcttttt ggttatgcca cagatattcg aagtttaagt cagggtcgtg
     2881 cgagtttcag tatggagttt ttggtgtatg cagtggtacc actaaaggtg aaatctgaaa
     2941 ttttgacaaa gttaggtcga ttttaataaa ttatttttcg atttgtcttt gacattaatt
     3001 gtttcttcag tgcatactcg cgcacttgaa acacctaagt ttacaaaagg ctctctgaat
     3061 taggaaattt ccaggaccgc aagggtccat ccagagatag ctttcgatgg gatgatggtg
     3121 ctgtttagtc agattttact agtatttacg ataccttaac aaaaagaaga gtgtcctatg
     3181 caaagtcaga aaatcagaat tcggttaaag gctttcgacc acaaactttt agatcaatct
     3241 acgaaagaaa tcgtagagac ggctcgccgt actggtgcta aaattgctgg tccgattcca
     3301 ttgccgaccc gtatcaatcg ttatacagtg ctgcgctcgc ctcacgttga taaaaaatct
     3361 cgtgaacaat ttgaggtgcg tactcataag cgcatgctgg atattttgga accaacacag
     3421 cagactgtcg atcagctcat gaaactcgac ctctcagctg gtgtagatgt tgaaattaaa
     3481 ctgtcagcaa tctgatagga gtaagttgtg agcgaagcta caaatgcagg tgctgaagca
     3541 ctaacaaata atacctcctc tgagcaaggg ctgaagctta atgggctttt tgcttttaaa
     3601 gaaggtatgt ctacaatttt caatagccaa ggtgaggctg taccagtgac agttcttcgt
     3661 tatgagcctt gggttatttc ccaaattaaa acaaatgaca aagatggata tgaggctatt
     3721 caggtggctt gccatcctaa aaaagcaaaa aactccaata aatctgaaaa aggtcatttg
     3781 gcaaaagctg gatttgaaaa tggagctcag tttgtaaaag aactccgtca ggcgcttcca
     3841 gaaggcgctt cccttggtgc tcaagtttct attgaaagcc ttgtgaaagg tgattttgta
     3901 aaaatcacct ctaagtctaa aggtaagggt tttgctggtt cagtaaagcg ttggaatttt
     3961 gctggaggtc ctgctagtca cggatcgcat tttcatcgtc gtcccggttc atctggtaac
     4021 aggacatggc cgggtcgggt catgccaggt aaaaaatacc caggtcagtt ggggtctgaa
     4081 actattacgg ttagaaatgt tgaaattgtt gaaatcaatg ctactgaaaa tgtagttttg
     4141 gttaaaggac cagttcctgg tgcaagaaac actttagtga aattggtgag agaatagtta
     4201 tggcagtagt taacgttcta aattggaaaa aagaaaaagt aggtcaagtt gatctggctg
     4261 caaatgtttt tgaagctcct gttaaaaagg atattcttca taatgtagtc cagtggcagt
     4321 tggcaagccg tcgtcaggga actcatatga ctaaaacaaa aggtcttgtg a
//
LOCUS       DATBTE010000316         3069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_178446, whole genome shotgun sequence.
ACCESSION   DATBTE010000316 DATBTE010000000
VERSION     DATBTE010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178446"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..530
                     /locus_tag="VIG33_11760"
     CDS             273..530
                     /locus_tag="VIG33_11760"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8271555.1"
                     /translation="MRKQIAVIEMVAGETIMEGNVVEDIDNTKNMAEYLNMSQSWARQ
                     ARMRGDGPPFLKIGRTVRYRKTDVDEWMIRKLRKNTIERAC"
     gene            complement(546..1793)
                     /locus_tag="VIG33_11765"
     CDS             complement(546..1793)
                     /locus_tag="VIG33_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY8271556.1"
                     /translation="MHLRKPLILTTITNTLIKTIQPKERPFEVRDEQLKGFLLRIQPS
                     GVMTYYAEYRRGKRIKVGRADAITPYKARALAREVLANACLGKDPAEKPKEDNASTFL
                     IFLTETYKPWILANLRTGEATYINIKRSFPELHSMPLRDISHLDIEKWRTRRLTEGVK
                     PSTVNRALDDLRACLNRAVEWEVIENNPIAKVKRSKIDSNPTPRFLTTSEEKALRQAL
                     DDREHRLRAERGSGNKWKAERGYPLLPSLSGVAYADHLKPAVLLSLNTGLRRGELFKL
                     KWSDINFEQNILTVKAETAKSGKTRHIPLNEEAALILKRWKTQEGLKGLHVFINNDGK
                     PFHDVRTAWKGVLDDADIKNFRWHDLRHTFASKLVMAGVDLNTVRELMGHSDYTMTLR
                     YAHLAPEHKAQAVAKLNTVATVT"
     gene            2046..>3069
                     /locus_tag="VIG33_11770"
     CDS             2046..>3069
                     /locus_tag="VIG33_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271557.1"
                     /translation="MGKATVKANELQTCLEHLDASRGARFFSLESDKEYSGVEEIKST
                     LQTVDPEKNPYVRIDQDKNEIIQLFFKDVRITGKLDLSNLDFRSLTFEPVTIEELVIN
                     GRKSKNGVINILKNSSIESFTISNSTFNQINMHNSEIRDWSITNTEISNMFKISTSLI
                     HGLFHFENIILHPPQEQSAYKVEQCIGSNNAQLILENFTLKNSGDFQLNQLKNLEKFA
                     VNSFDCQYDSRVSINACDMNGFETDCLGIEKFHFSDCSNYPISKASLCRTYATFRKNG
                     LCNNDKWGKRKGNIAKEQKSLYISLREEAKKKGNKQLEDDFYYCQMHWDLEERFKLWA
                     WLYRFFC"
BASE COUNT          928 a          618 c          656 g          867 t
ORIGIN      
        1 tccacaccgg tcgttaggct acgccacacc cctggaattc gtcgctcgct ggaattctac
       61 gatttccagc gatgccctgc gggctgtgat atgatcccaa aactctactt ttgagtggta
      121 ccaaaaactg gggccggtca gggagctgag gctacctaac cgcttacatt tggcttggta
      181 tgaggcaaag gagcaataca aaaccgcaaa aggaaagagg aggaaaaaat tgaagcagaa
      241 aagagatagc ttaacccgct tgaggatgga tattgagaaa gcaaatcgcc gttatagaga
      301 tggtcgcagg cgaaacaata atggagggta acgtagtgga agatattgat aatacaaaaa
      361 atatggcgga gtatctcaac atgagccaaa gttgggcaag acaggcaaga atgcgagggg
      421 atggcccgcc atttctaaaa atcggacgaa cagtcagata ccgaaaaaca gatgttgatg
      481 agtggatgat aagaaaactt cgcaaaaaca ctatagagag agcttgctaa tcaggctctc
      541 tttttttatg tcactgtagc cactgtgttc aacttggcaa cagcttgcgc tttatgttca
      601 ggggccaaat gcgcatatcg aagggtcata gtatagtcag aatgtcccat cagctccctg
      661 actgtattca aatccactcc tgccataacc aatttagatg caaaagtatg gcgaaggtca
      721 tgccatctga agttttttat atcggcatca tctaaaaccc ctttccaagc tgtccgaaca
      781 tcatgaaagg gttttccgtc attgttaatg aacacatgta aacctttaag cccttcctga
      841 gttttccagc gcttgagaat aagagcagcc tcttcattca ggggaatgtg acgggttttg
      901 ccggattttg cagtttccgc ctttacagtc agaatgtttt gctcaaaatt aatgtccgac
      961 catttgagct taaaaagttc tcctctgcgt aatccggtgt tcagagacaa aagaacagcg
     1021 ggtttaaggt gatctgcata tgcgacccct gacaagctcg gaagtagtgg gtagccccgc
     1081 tctgctttcc acttgtttcc actgcctcgc tcggctctta gcctatgctc acggtcatcc
     1141 aatgcttgac gcaacgcctt ctcttcactc gtagtcaaaa aacggggggt ggggttgcta
     1201 tcaatcttag aacgcttcac ttttgcaatt ggattatttt caataacttc ccactccacg
     1261 gcccgattta gacaggctct cagatcatcc aaagcccggt ttacggttga cggcttaaca
     1321 ccctccgtta gtctcctggt acgccacttt tcaatatcca gatgtgaaat atccctaagt
     1381 ggcattgagt gcagttctgg aaaagatcgt tttatgttga tataagtagc ctctccggtt
     1441 cggaggttgg ccaggatcca aggtttatat gtttccgtca gaaatatcag aaaagtactc
     1501 gcgttatctt ccttaggttt ttcggcagga tctttcccta gacatgcatt agcgaggact
     1561 tctctcgcta atgctcgcgc cttataaggt gttatggcat cagctcgccc tactttaatg
     1621 cgcttacccc gccgatactc agcatagtag gtcattactc cggaaggttg aattctaagc
     1681 agaaacccct tcagctgctc gtctcggact tcaaacggcc tctctttcgg ctggatagtt
     1741 ttgataagtg tgttagttat cgttgtcaat atcaacggct tccgtaagtg caatgtaagt
     1801 gcagattgtt aatacaatag cacatccata tgaacaacaa aagcctgaat aatatcagtg
     1861 tactgtaatt atagtgagct tttccaggtt tttggaatct cctaatgttc ggtgttgttt
     1921 ttcaaaaaat cgcgttcggg acgaaggggt cgcaggttcg aatcctgtca tcccgaccac
     1981 tttctttcaa gcctccttta caaacatagg gggcttttca cgcgaatttg aaatgtgagc
     2041 taaagatggg taaggcaaca gttaaagcaa acgaattaca aacctgcctc gaacatttag
     2101 atgcatcccg cggcgcaagg ttttttagtc tggagtctga caaggaatac agcggtgtcg
     2161 aagagattaa aagtacatta cagaccgtag atccagaaaa aaatccctac gttcgtatcg
     2221 atcaagataa aaatgaaata atccaacttt tcttcaaaga tgtaaggata acaggtaagc
     2281 ttgatctatc caatctggat ttcagaagtc ttacttttga accggttact atagaagaac
     2341 tcgtcataaa tggacgcaaa tccaagaatg gtgtcatcaa tatattgaag aattcttcaa
     2401 ttgaatcttt cactatcagc aatagtacct tcaatcagat taatatgcat aatagcgaga
     2461 ttagagattg gtcaataacg aatactgaaa tatctaatat gtttaagata tcaacctcac
     2521 tcatacatgg gttatttcat tttgaaaata taattttaca cccgccacag gaacaatctg
     2581 cttataaggt tgaacagtgc attggaagta ataatgctca gttaatatta gaaaatttta
     2641 cacttaaaaa cagtggagat tttcaattaa atcaacttaa aaatctagaa aaatttgcag
     2701 taaattcttt tgattgccag tatgattcaa gagtatcgat aaacgcgtgt gatatgaacg
     2761 gatttgaaac tgattgtctc ggaatagaga agtttcattt ttctgattgt agcaattatc
     2821 caatatcgaa agctagcctc tgtagaacat atgcgacgtt tagaaagaat ggtctctgta
     2881 acaatgataa atggggtaag agaaagggaa atattgcaaa agagcaaaaa agtctttaca
     2941 tctctctaag ggaggaagcc aagaaaaaag gcaataagca acttgaggat gatttttact
     3001 attgtcaaat gcattgggat ttagaagaaa gattcaagtt atgggcttgg ttatatagat
     3061 ttttttgtt
//
LOCUS       DATBTE010000317        11745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1887100, whole genome shotgun sequence.
ACCESSION   DATBTE010000317 DATBTE010000000
VERSION     DATBTE010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 11745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11745
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1887100"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..1066
                     /locus_tag="VIG33_11775"
     CDS             338..1066
                     /locus_tag="VIG33_11775"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY8271558.1"
                     /translation="MMYAHCVSKVNQKTTDIFSKISTDEGIRLFQDLANIRGEIVCKG
                     SGEDVYRLTVERANGRQELHCSVPFGFLKPKPESDILGNFFIGGERYFFKSSVRTDAD
                     IVILRMDTELFHLQRRQNYRIKIPENYPAALHLSSYNDTSINLKGQLADLSCGGCRVV
                     VAADLPLFEIGGHIDGKIIIKNRDPLAIEGILRYHKFEKSISNPRQIFGVEFISLTAL
                     MEGKLFALTMELHREFFSRLNANN"
     gene            complement(1090..1602)
                     /locus_tag="VIG33_11780"
     CDS             complement(1090..1602)
                     /locus_tag="VIG33_11780"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEY8271559.1"
                     /translation="MKNNTELNKSFFRFHLPHIHLNSVFGDDWFALKAEAFARFFGTP
                     VFLISQTFVVIVWISINVFGFAKFDIYPFILLNLAFSLQAAYAAPLILLAQTRQADRD
                     KAHAEADAQHREAIAQASEEREMFAAQQTAHLMELLKQNTALTEMTQQLSKRIEALTI
                     EVHSKILDKK"
     gene            1771..2478
                     /locus_tag="VIG33_11785"
     CDS             1771..2478
                     /locus_tag="VIG33_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271560.1"
                     /translation="MLYGKRAYKPQSRQSRLRSQKIILNKAFQYKYTLFLMTAIIGGA
                     TLFFAPAYYFINQNYVLFTNLAYDIQPALVTHLEREVVWLGIFMVLSLALISGMTLFI
                     GLRMTNNLLAPLVDMEKHMKKLMFGHWHIPDYKIANEDDFRDLAMTYDYFYRSLKANT
                     EAELKLIEKLSIDPQNREAYASWKNLIMIKRSRIGMPELQFEELNVQPLSRNGPIFKS
                     SESDVVSIEAERRRRVS"
     gene            complement(2423..2875)
                     /gene="smpB"
                     /locus_tag="VIG33_11790"
     CDS             complement(2423..2875)
                     /gene="smpB"
                     /locus_tag="VIG33_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEY8271561.1"
                     /translation="MSIVIIQENKTARFNYEVIDTFEAGLMLTGSEVKALREKNVQLK
                     DSYVSFRGDEAFLQNAHISVYKASSYNNHEPERLRKLLLNRSELDRIYDALREKGYSC
                     VPLKIYFKKGRAKLEIALVKGKKTHDKREALKKRDADAQLRSTLRRSR"
     gene            complement(2872..3714)
                     /gene="rsmA"
                     /locus_tag="VIG33_11795"
     CDS             complement(2872..3714)
                     /gene="rsmA"
                     /locus_tag="VIG33_11795"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEY8271562.1"
                     /translation="MSTARERLQEVQEALGIAAKRSLGQNFLVSDLVIEKIIKKAQDF
                     RPAVLIEVGPGPGALTYFLRKMECDFLVIELDRVLSQYWRDQGLNVVEGDALQMDWRT
                     FYQKPGKKVLVSNLPYQISSSIVVERCLDCSSAQEGLDHMVLMFQKEVAQRIAAKAKS
                     ENYGFLTVMAQTFWKTETVTEAGPRDFSPPPRVASRVLAFSRRLSSVQNPKAYLTFVK
                     SAFAQRRKLLKKNISSLCSQKGIAEEQLVSWITEMGFTETARAEELTPEQFVTLYKKF
                     GFET"
     gene            complement(3711..4757)
                     /gene="tsaD"
                     /locus_tag="VIG33_11800"
     CDS             complement(3711..4757)
                     /gene="tsaD"
                     /locus_tag="VIG33_11800"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY8271563.1"
                     /translation="MNRVLAIETSCDDTSVAIVEQTGWVHSVVCASQDLEHEPYGGIV
                     PEIACRNHSMALIPLIEEALKKANMTWADIPAIAVTNRPGLVGALIVGLVTAKSLAQA
                     RNIPFIGVNHLEGHLLAPFLKDAQYSPPEDFDYPYVGLAISGGHTSLYHILGVGNYEI
                     LGTTKDDAAGEAFDKFAKMAGLGFPGGARVDREAQKGNPKAYDFPRSMIHDDTFDMSF
                     SGLKSAGQRMLEQMGPEEVQKHLPDLCASFQEAIVDVLITKLEKAAKKFKAKRVILTG
                     GVSANSRLRARAEDWAKRKGFKLVIPPLRYCTDNAAMIGYVGVLRLNRGEVSSLDLGP
                     SPQSFPQDFKWAKL"
     gene            complement(4781..5497)
                     /locus_tag="VIG33_11805"
     CDS             complement(4781..5497)
                     /locus_tag="VIG33_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8271564.1"
                     /translation="MMQSVLPVSRLANGLVLSACLGLTGCLKTRTDVREIEQKQVIQQ
                     EVTKLQKTNADVSSRFSDVEDDLRTLRGRLEVLEHKKDQASQETDSVKRYATEQSADT
                     NKKILLLQEGLSNLEKTVFQLSAELNGLRAEKAALVAQNNAAQARVAAESKRNPYELG
                     QEFFEQKDWKKAILNYQRYRDENPNGKKIADATYKMGVSFQEMGMKDEAKTFFDELVS
                     KHPNSNEAKKARIRLKGLKK"
     gene            complement(5494..5802)
                     /locus_tag="VIG33_11810"
     CDS             complement(5494..5802)
                     /locus_tag="VIG33_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HEY8271565.1"
                     /translation="MRGLVIFVTMLIMLTSSCQSVPQPVEEYTLARAAIEAARAVQAP
                     RHSPGYWHQADEAYRQGRLLYKDRDYTKAREAFIKARIAAEKAENSARLIRQKTGDVL
                     "
     gene            complement(5818..6336)
                     /gene="pal"
                     /locus_tag="VIG33_11815"
     CDS             complement(5818..6336)
                     /gene="pal"
                     /locus_tag="VIG33_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY8271566.1"
                     /translation="MLKKLTFTFAALALTASCFVGCKGKQTKTDAETTPTTPVADTAV
                     DSTAMNFDASGSDSGKIPGLETVHFDYDKSNITAENKKILQGNADWVKKNTASKVQIE
                     GHCDARGSIEYNLALGERRANAVKAYLVSLGVPAARLSVISYGKEKPVESGDSEAAYA
                     KNRRANFLPVQQ"
     gene            6462..9404
                     /locus_tag="VIG33_11820"
     CDS             6462..9404
                     /locus_tag="VIG33_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271567.1"
                     /translation="MKIKSLFLFGAFILTAALFPCRGLSFEVPAEWKFKKISTAHFDV
                     IYNAEQQDLGQHYARQVEKAFSLLSPVFTEQPEKTLIIINDKTDATNGYATRLPYPHI
                     VTYPVLPGPQESLSESGDWSLELLSHEYTHILTFEPANGVFKILRGIMGSIASPNLLL
                     PSWWKEGLAVEAESLLGPKGGRLKSLYQDSIIRAMAENKSLLNYDVAQINEVIPRWPE
                     GMRAYLFGSIFWNQAVQDYGTGIMNNLNQRHSSRVPYFIEEPARDLLKDDYENFYHKA
                     LTETDKLAQGQIKTLRSLPLTEIHSLPSATNSDLKYSSAPAISSDGKYLAMISVDYKA
                     DRMMNIFVRDADTNMMTKKLQVEIKKEEEPLAPKTNKEDGPPSGSIQRISWFHHSPKL
                     IYDQLHYVNRIERYSDLYTYDVDTRKSKKLTTALRAREPSVSPDDDKVVFVRLEGGRT
                     RLGIYDIHSGKAEILYSPALQERISTPIFLDANTLIFALRDAQGTEGLWTYSFKDQTV
                     APILTSFEQARFPVLTKKGLMFTSSKNGVHNLYQASADLKKATPVTHVLTMVTTATLD
                     PETENIYATYMSATGPTVVMISKDIWQKTPQELPRIHGLFADRFKTFLAPTPGSEAPA
                     TNFEVTDYSPWGYMWPHYWIPYLWTSPEGGLVIQAQTSGFDPLKKHSYSLLIDWNTYL
                     QALDWSADYLNNVTNLPLQVLGAQTHSYLVTRDNLIKDTLASVAGLPDIWGFSEKLSL
                     GLGWRYLERTLGGTGTTKRTGPYATLTYKNSSQGGDQFTPQEGGGAYLMAVNYVEGAG
                     LMSHSQFQTGGVYYFSQFLPKLHALVIRGSLLYTPEKIPPIYGTQSDSMDFAGQDLSP
                     HFLARGYSTGQFIGHNMYNLNLEYRFPVQDVYHGQGTHPFFARRLYGAVITDGLATDG
                     FAYNSTNIPTQVKASEIFWSYGIEAHLETTVGYEFPLNFVAGFYKGANEKYAPDSTLG
                     LAIQFSGF"
     gene            9485..10003
                     /locus_tag="VIG33_11825"
     CDS             9485..10003
                     /locus_tag="VIG33_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271568.1"
                     /translation="MEKISWIKELVKAEQQMEESGLVDMSFGFDPERVLVTESIQFLL
                     NLKTEFVDASSTFNELKPSALGRIKIYGIAKTHADFMLFRNGFKMIFALKAPGQISVR
                     FNFIGTNYIPTPGAAEPHTPAANANVMEENIVEAKWAAFEELIWTYRGEPMKLEYMVR
                     HYLTQFIRESAK"
     gene            complement(9976..11217)
                     /locus_tag="VIG33_11830"
     CDS             complement(9976..11217)
                     /locus_tag="VIG33_11830"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEY8271569.1"
                     /translation="MKIWAWILLLLVFGSKISFAFLGLPSQIHMLVFQHGLHPYLNII
                     LNFLFGMPIVFYMLHHSQKLEGMTKPYRTLLVFLLILLFFETLSQFLFFDSHKGFYIW
                     AAVISTFWMILLFGIFLPLLLEPLSAARLLSTWSINFVVLSLVLWLVRPDLTFKGGRF
                     IGVFKHIPYMVTCASVGVIFTLGVVQMEKNRLRRFWQIFGIFLSLFALILTGTRSALA
                     AVLMAVILSILRAPTPNLARRYFKYSAVLVMSVTLLLFGVKIMDYAKDIATGKKALLE
                     REAQDGVASRMEEIERGWEYFQTSPWIGQGLLSKFSGKEELEVSSYNSFKDPHNIIAS
                     AGVVGGWPFIMWTVFFLLWLISMSVKAFISKDAVMSIFGVYALVQIPILIIYHWHLSL
                     GGMADRFYWLVFGYLALSRMN"
     gene            complement(11224..>11745)
                     /locus_tag="VIG33_11835"
     CDS             complement(11224..>11745)
                     /locus_tag="VIG33_11835"
                     /inference="COORDINATES: protein motif:HMM:NF022007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEY8271570.1"
                     /translation="VIFWSSISNSSPLNDLGSIGDIKMPAQASEYIFRSSPKESLISI
                     QLLGEVQKPGIYYVPSNTDLLKVLTLAGGSTHGGNISEVMVRKNEPEKWATLRSQALA
                     EHRGTYEVDLEELIRRGGSDSLKMNHDDLVYVPPREAMFSPETTKTVTLVSVIMSIAL
                     SGLLIGKYSHQKE"
BASE COUNT         3285 a         2831 c         2414 g         3215 t
ORIGIN      
        1 ccatctttat cttcatgttc taaagtctaa gggaggtttc agtttgcgaa aacaaaaaag
       61 ccatgggttt catgccatgg ctttgtttta atagtatttt atagctttac taaggtgggc
      121 ccgtctcccg gtggtggacc aactcacgaa gttcccggtg aagcataaac tcgtgacgta
      181 cggatggatt aattaataac actcgcgtcc tcctgtgaga ccagttgtta tgagtggcca
      241 tcataactta cttatcggct agaccttcgc cgagttgagg gctgttttaa ttatttttaa
      301 agattcccat tttgaagctc ttctcttcac aaaaggcatg atgtatgctc actgcgtgag
      361 taaggtgaat caaaaaacga ctgatatttt ttcaaaaata agcaccgatg aagggatccg
      421 tcttttccaa gaccttgcca atattcgtgg tgaaattgtc tgcaagggaa gtggtgagga
      481 tgtctatcgt ctcaccgtcg aacgcgccaa tggaagacaa gaactccatt gctcggtgcc
      541 cttcggtttt ttaaagccaa aaccagaaag tgatatttta ggaaatttct ttattggtgg
      601 ggaacgctat ttctttaaga gttccgtacg caccgatgct gacatcgtca ttcttaggat
      661 ggacacggag cttttccacc tccaacgccg ccaaaattat aggattaaaa tccctgaaaa
      721 ttatccagca gccctgcatc tttccagtta caatgacacc tctattaact taaaagggca
      781 gctcgctgat ttaagctgtg gcggctgtcg tgtcgtcgtc gcggccgatc ttcctctttt
      841 tgaaataggg ggccacatcg acggcaagat cataattaaa aacagagatc ctttggcaat
      901 tgaaggtatt cttcgctatc ataaatttga aaagagtatt tcaaatccaa gacaaatatt
      961 tggtgtcgag tttatttctc ttaccgcctt aatggaggga aagctctttg ccctcaccat
     1021 ggaacttcac cgggagttct tctcaagatt gaatgccaat aactaagcaa agagtgtcag
     1081 cgatcccact tattttttat ccaatatttt actgtgcact tcgatcgtca gggcctcaat
     1141 gcgtttactc agttgctggg tcatttcggt tagcgctgtg ttttgtttaa gcaattccat
     1201 caaatgagcc gtctgctgag cagcaaacat ttcccgttct tcgctggctt gagcaatggc
     1261 ctctcggtgc tgggcatccg cttccgcatg agctttatct cgatctgcct gtctggtctg
     1321 agctaataaa ataagtggcg cagcgtaggc tgcctgtaga ctaaaggcca gattgagtag
     1381 aatgaacgga taaatatcaa atttggcgaa accaaaaaca ttgatggaaa tccaaacaat
     1441 tacaacgaag gtttgactaa ttaagaagac cggggttcca aaaaagcgag cgaaggcctc
     1501 cgcctttaaa gcaaaccagt catctccaaa cactgaatta agatggatgt gaggtaaatg
     1561 aaatcgaaag aaactcttat taagctcagt attatttttc acaataaatc tccaagttcc
     1621 ataggaagcc ccgcacagag tctaaggtcc taatatattt ctctttttat cgtccaaaga
     1681 cctgctaaaa gaccattcta agtcccagtt tgactcattt tgagaataat tggactttaa
     1741 ataaaactca atctgccgat aactatatat atgttgtacg gaaaaagagc ttataagcct
     1801 cagtctcgtc agtctcgtct tcgttctcag aaaataattc tgaataaggc cttccagtat
     1861 aaatacactc tatttttaat gacggcgatt attggcggag ccacattgtt tttcgctccg
     1921 gcttattact ttatcaacca aaactatgtt cttttcacga acctggccta tgatattcaa
     1981 cccgccttgg tcactcatct ggaacgcgaa gtggtttggc ttggaatctt catggtcctt
     2041 agtctagccc tcatttcagg aatgactttg ttcattgggt tgcggatgac taataatctt
     2101 ttggctcctc tagtggatat ggaaaagcac atgaaaaaac tcatgtttgg ccactggcat
     2161 attccggatt acaagatcgc caatgaagat gatttccgcg atttagccat gacctacgat
     2221 tatttctatc gttctttaaa agccaatacg gaagcagaat tgaaattgat tgagaaactt
     2281 tcgatcgatc cgcaaaatcg tgaagcctac gcctcttgga agaacctaat catgattaaa
     2341 agatcaagaa ttggaatgcc agagcttcaa ttcgaagaac tcaatgtaca acccctctct
     2401 cgcaatggac ctatttttaa atctagcgag agcgacgtag tgtcgatcga agctgagcgt
     2461 cggcgtcgcg tttcttaagc gcctcacgtt tatcgtgagt tttcttacct ttcaccaagg
     2521 caatctctaa cttcgcacgg ccttttttaa aatagatctt caaaggcacg cagctataac
     2581 ctttttcacg gagtgcatcg taaatacgat cgagctccga acggtttagg agaagctttc
     2641 gcaatctttc aggttcgtga ttattgtaac tgctggcttt ataaacgctg atatgggcat
     2701 tttgcaaaaa agcctcatct ccgcggaaag acacatacga atctttcaat tgaacatttt
     2761 tctcgcgcag agctttcact tcgcttccag tcaacatcaa gcccgcttcg aaggtgtcga
     2821 tgacttcgta gttgaatcga gctgttttat tttcctggat gattactatg ctcatgtttc
     2881 aaaaccaaat tttttataaa gggttacaaa ctgctccgga gttagctctt cggctcgggc
     2941 ggtctcagta aaacccatct ccgtaatcca gcttactaac tgctcttcag ctattccctt
     3001 ttgactgcaa agagaagaaa tatttttctt taaaagctta cgacgctgag caaaggccga
     3061 cttgacgaaa gtcagatacg cctttgggtt ctgcactgaa ctgagcctac gactaaaggc
     3121 tagaaccctt gaagccactc tcggaggcgg tgaaaagtcc cgcggcccag cctccgtcac
     3181 cgtttcagtt ttccaaaaag tctgtgccat tacagttaaa aaaccataat tctcagactt
     3241 ggccttcgct gcaattcttt gtgccacttc tttttgaaac atgagcacca tgtggtccag
     3301 cccctcctgt gcagaggagc aatccaaaca ccgttccacc acaatgcttg aggaaatctg
     3361 gtacggaagg ttgcttacga gaactttttt gccgggcttt tggtaaaacg tccgccaatc
     3421 catttgcaga gcatccccct ccacgacgtt taaaccttga tcccgccaat actgagaaag
     3481 cacccgatcc aactcaatca ctaaaaagtc acattccatt tttctgagaa aataggtcaa
     3541 agccccaggc cccggtccca cttcgatcag cacagctggc cgaaagtcct gagctttttt
     3601 aataattttt tcgatgacca aatcgctgac taaaaaattc tgacccaagg agcgtttggc
     3661 ggcaataccg agagcctctt gcacttcctg aaggcgttca cgggctgtac tcatagttta
     3721 gcccatttga aatcttgggg aaagctctgc ggagaaggtc ccagatctaa actagagact
     3781 tcaccccgat tcagccttaa gacaccgaca taaccgatca tagccgcatt gtccgtacaa
     3841 tatctcagag gtggaatcac cagtttaaaa ccttttcgct tcgcccagtc ttcggcccgg
     3901 gcacgcagtc gggagttggc actcaccccc ccagttaaaa tcacccgttt cgctttaaat
     3961 ttcttcgccg ctttttcaag tttcgtaatc aggacatcca cgatggcctc ctgaaaagaa
     4021 gcgcacagat ctggcaaatg tttctgcacc tcttcaggtc ccatttgctc aagcattctt
     4081 tgcccagcgg acttaagtcc tgaaaagctc atatcaaaag tatcgtcatg aatcatactg
     4141 cgaggaaaat cataagcctt aggatttcct ttttgcgctt cccgatcgac gcgagcgccg
     4201 ccgggaaagc ccaagcctgc catctttgca aatttatcga aggcctcacc tgcggcatca
     4261 tccttggtgg tacccaaaat ctcataattt ccaacaccaa gaatatgata aaggctggta
     4321 tgccccccac tgatagcaag tcccacataa ggatagtcga aatcttccgg tggggaatat
     4381 tgggcatctt taagaaaagg agccagcaaa tgcccttcaa gatggttcac accaataaac
     4441 ggaatattgc gagcttgcgc caggctcttt gcggtcacta gtcccactat caaagcccca
     4501 accaaacccg gacgattggt cacggcaatg gcaggaatat ccgcccaagt catattggct
     4561 tttttcaaag cctcttcaat caaagggata agggccatcg agtgattacg gcaggcgatt
     4621 tcaggaacaa ttcccccata gggttcatgt tccaagtctt ggctcgcgca aaccaccgaa
     4681 tgaacccaac ctgtctgctc aacaatagca acagaggtat cgtcacagct cgtttcaatc
     4741 gcgagaactc gattcatttt tcacactctt tttaagcaga ctatttttta aggcctttca
     4801 aacgaattcg ggctttcttt gcttcgttcg aattgggatg tttagaaacg agttcatcaa
     4861 aaaaagtctt cgcctcatct ttcattccca tctcctgaaa tgaaacaccc attttataag
     4921 tagcatccgc gattttcttc ccgttagggt tttcatcgcg atatctttga taatttaaaa
     4981 tcgctttttt ccaatctttt tgttcgaaaa attcttggcc aagttcataa ggatttcgct
     5041 tggattccgc agcaactctc gcttgagcgg cattattttg ggccactaaa gccgcttttt
     5101 ccgcgcgaag cccattcaat tccgcactca gttggaaaac agttttttca agattgctca
     5161 aaccttcttg aagaagaaga atctttttat tggtgtctgc cgattgttct gtcgcatatc
     5221 gttttacaga atcggtctct tgagaagcct ggtctttttt atgctccagc acttccagac
     5281 gaccccgaag agttctcaag tcatcttcca catcagaaaa acgactggaa acatccgcat
     5341 tggttttctg cagctttgtc acttcttgct gaatcacttg tttttgttca atttcccgaa
     5401 catccgtacg agttttgaga cagccggtca ggccgagaca agcgctcaaa accaatccat
     5461 ttgccaatct gcttacaggg agcaccgact gcatcataaa acatctcctg ttttctgtcg
     5521 aatcagacga gctgaattct cagctttttc cgcggcaatt cttgctttaa taaaagcctc
     5581 gcgtgctttt gtataatctc ggtctttata gagcaaacga ccttgtcgat aagcttcatc
     5641 agcttggtgc caataccctg gcgaatgacg gggagcttga accgcgcgag ccgcttcgat
     5701 agcagctctt gctaaagtat attcttcgac cggctgggga actgactgac agctcgaggt
     5761 gagcataatc agcatagtta cgaaaataac aagtcccctc acccgacgat ccttttacta
     5821 ttgctgaact ggaaggaaat ttgcacgacg gttttttgcg taggcagctt cactgtcccc
     5881 tgattcaaca ggtttttctt ttccatagct gatcacagat aaacgagccg ctggaacacc
     5941 aaggcttaca aggtaggctt ttacagcgtt tgcacgtctt tcaccaagag ctaaattgta
     6001 ctcgatagaa ccacgagcat cacaatgtcc ttcaatttga accttggaag ccgtgttctt
     6061 tttaacccag tccgcattcc cttgaaggat ttttttattt tcagcagtaa tgtttgattt
     6121 atcataatca aaatgcacag tctcaagtcc aggaatttta ccactatcac tgcctgaagc
     6181 atcaaagttc atagcagttg aatccacagc tgtgtcagcg actggagtgg taggtgtagt
     6241 ttccgcatct gttttcgtct gcttgccttt acaacccaca aaacaacttg ctgtcaaagc
     6301 caatgctgca aaagtgaacg tcaatttttt taacatcgtt cttcctcctt aaatgttttt
     6361 cttaatctct gaaactttaa ggcatgacag attattgtca aaccatatca aagagttgat
     6421 tcgatattga tgttgcgttg ccatgcggtg ataatgaagg gatgaaaatt aaatctttat
     6481 ttctttttgg cgcctttatc ctcactgctg ctctttttcc ctgtcgaggc ctctcctttg
     6541 aagttcctgc agagtggaaa ttcaaaaaga tatctacagc ccattttgat gtcatttata
     6601 atgctgagca gcaagatctc ggacaacact acgcccgaca agtcgaaaaa gcattttcat
     6661 tgctgtcccc tgttttcact gaacagcctg agaaaacact tattattatt aatgacaaaa
     6721 ccgatgcgac gaatggttat gccactcgcc ttccctatcc ccacatcgtg acttatcctg
     6781 ttcttcctgg acctcaggag agtttgagcg aaagtggtga ctggtccctg gaacttctct
     6841 cgcatgaata cactcatatt ctcactttcg aacccgccaa tggagttttt aaaatcctgc
     6901 gtggtattat gggaagcatt gcctcgccca atctcttgtt gccaagttgg tggaaagaag
     6961 ggttggccgt agaagctgag agtctattgg gtcccaaagg gggacgccta aaatccctgt
     7021 accaagactc catcatccgc gccatggctg agaataaaag tctccttaac tacgacgttg
     7081 cacagatcaa tgaagttatt ccccgttggc cagaggggat gagggcttat ttgtttggct
     7141 ctattttctg gaatcaagcg gtccaagatt atggcacagg aatcatgaat aacttgaacc
     7201 agcgtcactc ctccagagtc ccctatttca tcgaagaacc cgcacgtgac cttctgaagg
     7261 acgattacga aaacttttac cacaaagccc tgaccgagac cgataagtta gcgcaggggc
     7321 agattaagac tctgcgttct cttcccctta ccgaaatcca tagcctgcca tcggcgacaa
     7381 attcagacct caagtactca tctgccccag ccatcagcag cgacggaaaa tatctggcga
     7441 tgattagcgt ggattataaa gccgatcgca tgatgaatat tttcgttcgg gatgcagata
     7501 ccaacatgat gactaaaaaa ctccaagttg aaataaaaaa ggaagaagaa cctttagctc
     7561 ctaaaaccaa taaagaagat ggtccccctt cgggaagtat tcaaaggatt tcgtggtttc
     7621 atcactctcc taaactcatc tatgaccagc ttcattacgt caatcgcatc gagcgctatt
     7681 cggatcttta cacttacgat gttgataccc gaaaaagtaa aaaactgacc accgctcttc
     7741 gcgcccgtga accttctgtt tctccggacg acgacaaagt ggtctttgtg agacttgaag
     7801 gcggaagaac ccgactggga atctatgata tccacagtgg aaaggcagag attctatata
     7861 gtcctgccct ccaagaaaga atctccacac cgattttctt agatgcgaat acgcttattt
     7921 ttgcactgag agacgctcag ggaaccgaag gtctttggac ttactcattt aaagatcaaa
     7981 ccgtcgctcc tattctaacc tcttttgagc aagcgcgttt tccagtttta actaaaaaag
     8041 gactcatgtt cacgtcttcc aaaaatggcg ttcacaattt gtatcaggct agtgcagatc
     8101 tgaaaaaagc aacgcctgtg actcacgttt tgaccatggt tacgaccgcc actctggatc
     8161 ctgaaactga aaatatttat gcgacttata tgtcagccac gggtcctacg gtggtgatga
     8221 tttccaaaga catttggcaa aaaactcctc aagaactgcc gcgcatccac ggtctcttcg
     8281 cggatcgttt caaaaccttt ctcgcgccaa cacccggctc ggaagctcca gcaacaaatt
     8341 tcgaagtcac agattatagt ccttggggct atatgtggcc tcattattgg attccttatc
     8401 tttggacttc acctgaaggc ggtctggtca ttcaagcgca aacctcaggc tttgatccat
     8461 tgaaaaaaca cagttacagt cttttgatcg actggaatac ttatctccaa gcccttgatt
     8521 ggtccgccga ctatttaaac aacgtcacca atcttcctct ccaagtgctc ggagcccaaa
     8581 ctcactctta tttggtgact cgcgataatt taattaaaga caccttggcc tccgttgccg
     8641 gattgccgga tatttggggc ttcagtgaaa agctcagttt gggtctaggc tggcgctatc
     8701 ttgaaagaac tttggggggc actggaacca caaagcggac cggtccttat gcgactttga
     8761 cctataaaaa tagctcccaa ggtggagatc agttcactcc gcaagaaggc ggtggggctt
     8821 acttgatggc ggtgaattac gttgagggag ctggacttat gtcccactcc caattccaaa
     8881 ctggtggtgt ttattatttc tctcaatttt tgccaaagct gcacgcccta gtgatccgtg
     8941 gaagtttgct ttacactccc gagaaaatcc cgccaattta tggaactcaa tcggactcga
     9001 tggattttgc cggtcaagat ttgagcccac actttttggc ccgaggctat tcgaccggcc
     9061 agttcattgg ccacaatatg tataacctca atcttgagta tcgttttcca gtacaggacg
     9121 tgtatcacgg acaaggcact catcctttct tcgcacgtcg tctttatggc gctgtgatca
     9181 cagatgggct tgcgacagac ggttttgctt acaattccac taacattccc acgcaggtta
     9241 aagcttcgga aatattttgg agttacggta tcgaggctca ccttgaaacc acagtgggct
     9301 atgaattccc actgaatttc gtagctggct tctataaagg tgctaacgaa aaatatgctc
     9361 ctgacagcac tctgggtcta gcaatccaat tcagcggctt ttagcgcgcc acgtttaagc
     9421 gaaggccttg acaaaaattg aggtcgtcac gaacactgga agagagttta ttcatggagg
     9481 attaatggaa aaaatcagct ggattaaaga acttgtgaaa gctgaacagc aaatggaaga
     9541 atcaggctta gtcgatatga gctttggttt tgacccagag cgagtcttag ttactgaaag
     9601 catccagttt cttttgaatc ttaaaacaga attcgtcgat gcctcctcta cttttaacga
     9661 actaaagccc tccgccttgg ggcgaattaa aatttacgga atcgccaaaa cccatgccga
     9721 ttttatgctc ttccgtaatg gttttaaaat gatctttgct cttaaagctc caggtcaaat
     9781 cagcgtgcgt ttcaatttca tcggaaccaa ttacattcca actccaggag ccgcggaacc
     9841 tcacactccc gcagccaatg ccaacgtcat ggaagagaac atcgtggaag caaaatgggc
     9901 cgcatttgaa gaacttattt ggacttatcg gggtgagccc atgaagttgg aatatatggt
     9961 tcgccattat ctcactcagt tcattcgcga aagcgctaag taaccgaata ccagccaata
    10021 aaaacgatcc gccattccgc ctaaagacaa atgccaatga taaatgatga gaataggaat
    10081 ttgcacaagg gcgtaaactc caaaaataga catgacagca tccttagaga taaatgcttt
    10141 tactgacatg cttatcagcc aaagcaaaaa aaacactgtc cacataataa aaggccatcc
    10201 gcccaccaca cccgccgaag caataatatt atggggatct ttaaaggaat tgtaactgct
    10261 cacctccagc tcttctttac ctgaaaattt tgaaagcaag ccctgcccta tccaggggct
    10321 ggtttgaaag tattcccaac cccgctcgat ttcttccatt cggctggcga ctccatcttg
    10381 ggcttcgcgt tcaagaagtg cttttttgcc cgtggcaata tctttagcat aatccattat
    10441 ttttacgcca aaaagaagaa gtgtcaccga catcaccaag acggctgaat atttaaagta
    10501 acgtcgtgcc aggttcggtg tgggtgcacg gagaatcgac aatatcaccg ccatgagaac
    10561 tgccgccaac gccgagcgag ttccggtcag gatcagagca aataagctca agaaaatccc
    10621 aaaaatctgc caaaaacgac ggagtcggtt cttttccatt tggacaactc ccagagtgaa
    10681 gatcactccg accgaagcac aggtcaccat ataaggaatg tgtttgaaaa ctccgataaa
    10741 acgtccccct ttgaaggtca gatccggcct taccaaccac agcaccaagg aaagcactac
    10801 aaaatttatg gaccaagtac tcagcaaacg cgcggcactc aagggctcca gcagaagggg
    10861 caaaaaaatc ccgaaaagga ggatcatcca aaaagtggaa atcacagccg cccatatata
    10921 gaaaccttta tggctatcga aaaataaaaa ttgtgacaga gtctcgaaaa acaacaagat
    10981 caacaaaaaa accaaaaggg tcctataagg cttcgtcatc ccctccaact tctgcgaatg
    11041 atgaagcata taaaaaacga tgggcatccc aaaaaggaaa ttcagaataa tattaaggta
    11101 cggatgaaga ccatgttgaa agaccaacat atgaatttgc gaaggaaggc ccagaaaagc
    11161 gaacgatatc ttggaaccaa aaaccaataa taaaagaatc caggcccaga ttttcattgg
    11221 tgactactct ttctgatggc tgtacttgcc gatcaacagt ccactgagag caatactcat
    11281 aatgacagaa actaaagtga ctgttttagt ggtctcgggg ctaaacatgg cttctcgggg
    11341 aggtacataa accagatcat cgtgattcat ctttagagag tcgctccctc ctcggcgaat
    11401 cagttcctcg agatcgacct cataagttcc acgatgttcc gccagagcct gagagcgcag
    11461 tgtggcccac ttctccggtt cgtttttacg aaccatcact tcagaaatat ttccgccgtg
    11521 agtacttcct cctgcgagag tcagaacctt taagagatcg gtattgctgg gtacataata
    11581 aatccccggt ttctggacct cgcccaggag ttgaatggag ataagggact ctttcggaga
    11641 agatctgaag atatactccg aggcctgtgc gggcatttta atatccccaa ttgaacccaa
    11701 atcatttaag ggcgatgaat tagaaataga actccaaaaa attac
//
LOCUS       DATBTE010000318         6067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_247316, whole genome shotgun sequence.
ACCESSION   DATBTE010000318 DATBTE010000000
VERSION     DATBTE010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247316"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VIG33_11840"
     CDS             <1..400
                     /locus_tag="VIG33_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEY8271571.1"
                     /translation="ELVLKKATLGIYEEKDMQGLDPLLRQKYFNRIKGSSKRPKEERF
                     QAKDEIHKLLHFAYFNLMNETYIFKHKFNIIFCRNVLIYFDEPTTKRVIDNLTGALAT
                     GGHLILGHSESGNVKHKDLKPLSRAIYQKL"
     gene            408..1466
                     /locus_tag="VIG33_11845"
     CDS             408..1466
                     /locus_tag="VIG33_11845"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEY8271572.1"
                     /translation="MSKIRVLIVDDSAVIRKLLEKFLSSSADIQVVGSAADPYIARDK
                     LVELKPDVMTLDVEMPRMDGISFLEKVMQHMPTRTIIFSSLAKTGSETYFRALEAGAI
                     EILEKPSIDVSQSMETLAADIVEKVKNVARARISPVHKIVMPGTPVKKTSGGALARTT
                     HQLIAVASSTGGTEALKVLLAGMPANIPGTLIVQHMPPGFTKSFADSLNKMFPFEIKE
                     AQEGDQVIPGRVLIAPGNFHMELYRNGAFYHVKLHQAPPLHSVRPAADYMMKSVAKFV
                     GKNACGIVLTGMGKDGAEGLLEMKKAGAYTVAQNEETCVVYGMPQAAFNLGATDKVLP
                     LDKIADDVIQKINAKDAA"
     gene            complement(1475..2665)
                     /locus_tag="VIG33_11850"
     CDS             complement(1475..2665)
                     /locus_tag="VIG33_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(A) polymerase"
                     /protein_id="HEY8271573.1"
                     /translation="MTTKPHLHEDWIDPYARDIVRILQDEGFETYLVGGCVRDLLAGI
                     HPKDFDIATNAHPNQVRRKIPQAYVIGKRFRLVLVRRGDQQFEVATFRRAISAEELEG
                     ADSEVIGDNYFGSCEEDAKRRDFTINALFYDPIQHKILDYCDGLKDVENRTLRMIGDS
                     VTRFVEDPIRILRAVRLTHKLSFIIEPQMRAAIVQCAPELKRAVLPRRREEYIKILRL
                     PDPILAFTELHDLGVLENILSGLYAIFKDSEKMAVFETHLRRLKFCGIRFDDPTELLS
                     GFMFAFLKAHYGENSWNLAQIQNDDRLQFFLREELGMFKQEQATFFKALQLMHHFQRV
                     DLYLRKGERRQLGFFRNESVPLAMKLAQIDYSVAGTLLMFWQGQAKRFEGKTALNEGQ
                     EIQH"
     gene            2759..3574
                     /locus_tag="VIG33_11855"
     CDS             2759..3574
                     /locus_tag="VIG33_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8271574.1"
                     /translation="MFYLDLYNYQIIGPENGRRWVFVHGLMGYGANWRKIISSLESTE
                     RVLCFDQRGHGRSQKPAEGYTPEDYSEDILKITEELGWDKFILVGHSMGGRNVLNFAY
                     RYPEKIIKLVVEDIGPEAAPNAVEYYEKLLEMVPTPFPNREAARSFFQTEFLEKAKTR
                     ENPQVLAQFLYANIEDKADGTVDWRFYKQGILDSVSDGRNKDRWHEVISLKVPTLWIH
                     GAQSREFSRENFERVLDSNELITGVEIPNAGHWVHSEQPALFLQALKDFAGGF"
     gene            3667..5316
                     /locus_tag="VIG33_11860"
     CDS             3667..5316
                     /locus_tag="VIG33_11860"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8271575.1"
                     /translation="MNFSEFNLDPSLKSAIEKLNYAECTPIQELSIPVTLEGKDVAGL
                     AQTGTGKTAAFLLPIMERILRARPLAPGATEETKVLAAKRAFSDWKPQNFVLILVPTR
                     ELAEQVQESVEKFSEFSGLRGFAIYGGTGYDKQKEALKNGVEFIVATPGRLIDLYKEH
                     LVDLKQVRAIIFDEADRMFDMGFKEDMKYILQRVPKERQFLVFSATLNFDVLNTAYQF
                     GADPVEINISRDSAKAENVKDEIFHVGQDEKGQHLLSLLKLHKPRQAVIFTNFKVNVE
                     KITRFLNNNSIPAMAISSLLTQAQRTKVMQQFKAENDLNVLVATDVAARGLDVQGVDM
                     VVNFELPMDSESYVHRIGRTGRAGATGLAFSLVSDRDVDSLGRIEEYLKHKVEVGYLE
                     DKDLLKDFKPFVWSDSSYSRSYESRDGGGAGGRQDRGPRRGAGPRTGGTGGGGRSPRR
                     DDQQRRDHGPRRSDNRTTSSAGPRREGEQRPQHKRGPEHRSDQRRHTPHKPAAHQGNG
                     QGQRPQQAQPHRGPKKPHSAQVAKQKSIGQRVTGFIKKLFS"
     gene            complement(5350..>6067)
                     /locus_tag="VIG33_11865"
     CDS             complement(5350..>6067)
                     /locus_tag="VIG33_11865"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8271576.1"
                     /translation="CAAHFEDYFTRGGFPEVVEGEPQGLYLRELFDKIVGRDIVQRHN
                     VRIPKLLKELGVYMIQQSGSVISYQQLQRAFGYSSINTVRNHLAYLQESYLLYEVSAY
                     SYKLKERSTLPKKNYACDIGMMNSLSTKATDDFGMKLETLVFLHLRRKTKNIFYLKSS
                     QYDIDFAVTEDRKISSLIQVCFNISDERTRKRELSSLLNAAKKWKVTNLKIITWSNSE
                     ELHLEGVEIQVIPAWKFCLS"
BASE COUNT         1657 a         1295 c         1454 g         1661 t
ORIGIN      
        1 tgagctggtt ttgaagaaag cgactctggg gatttatgag gagaaggata tgcaagggct
       61 agatcccttg cttcgccaaa aatattttaa tagaataaaa ggatcctcaa agagaccgaa
      121 ggaagaacgg tttcaggcca aggatgaaat ccataaatta cttcactttg cctattttaa
      181 tttgatgaat gaaacttata tttttaaaca taagtttaac atcatttttt gtcgcaatgt
      241 tttgatctat tttgatgaac ccactacaaa gcgagtgatt gacaatttga ccggtgcttt
      301 ggctacaggt gggcacctca ttctgggtca ttctgaatct ggcaatgtga aacataaaga
      361 ccttaagccc ctctctagag caatctatca aaagttgtag gtgatacatg agtaaaatac
      421 gtgttcttat agttgatgac tcagcagtga ttcgaaagct tttggaaaag tttctttcct
      481 cttcggctga tattcaagtg gtgggctccg ccgccgaccc ttatatcgcc cgtgataaac
      541 tggttgaatt gaaaccggac gtgatgactc tagatgtgga aatgccccgt atggatggaa
      601 ttagcttcct tgaaaaagtg atgcagcata tgccgactcg cacgattatt ttttcaagtc
      661 tggcgaagac tggttccgag acctatttcc gcgctctaga agcgggcgct atcgagattc
      721 tagaaaaacc ttccattgat gtcagccaat ccatggagac cttggctgct gacatcgttg
      781 aaaaagtgaa gaatgtggct cgagctcgga taagcccggt ccataaaata gttatgccag
      841 gaactcccgt aaaaaaaact tccggaggtg ctttggcccg taccactcat caattgattg
      901 ccgtggcttc ttctaccggt ggaactgaag ctttaaaagt tttactcgcg ggaatgcccg
      961 caaatattcc gggaacttta atagtgcaac atatgccacc gggctttacg aagtcttttg
     1021 ccgacagcct taataagatg tttccttttg aaatcaaaga agctcaagaa ggagatcaag
     1081 tgatcccagg tcgcgttttg atcgctccag gaaacttcca tatggagctt tacagaaatg
     1141 gggccttcta tcatgtgaag ctccatcagg cacctccgct tcacagtgtt cgtccggcag
     1201 cggactatat gatgaaatca gtggcgaaat ttgtcggtaa aaatgcttgc ggtatagtgc
     1261 ttaccggaat gggtaaggac ggcgctgaag gtctacttga aatgaaaaaa gcgggtgctt
     1321 acacggtagc tcagaatgaa gagacctgcg tggtttatgg aatgcctcaa gccgccttca
     1381 atctgggggc aacggataaa gtactgcctt tggataaaat tgccgacgac gtgattcaaa
     1441 aaatcaatgc gaaggatgcg gcttaaagca cagcctaatg ttgaatttcc tgaccttcgt
     1501 tcagagccgt tttgccttca aaccgtttgg cttgtccttg ccaaaacatg agaagcgtgc
     1561 ccgcgactga ataatcaatc tgagcgagct tcatggcgag aggtacggat tcattgcgga
     1621 agaatcccaa ttgacggcgt tcacctttgc gcaggtacaa atcgactctt tgaaaatgat
     1681 gcattaactg caaggcctta aagaaggtgg cttgctcttg tttgaacata ccgagctctt
     1741 cacgcagaaa gaattgaagg cgatcatcat tttgaatttg agctaaattc caggagtttt
     1801 caccataatg agctttgagg aatgcaaaca taaaacctga aagcaattca gtgggatcat
     1861 caaaacggat tccacagaat ttcaagcgcc gcagatgcgt ttcaaaaacc gccatttttt
     1921 cagaatcttt aaaaatcgca tagagaccgg aaagaatatt ctccagcaca cctaagtcat
     1981 gcagttcggt gaaagctaga atcggatctg gtaaacgaag aattttgata tactcttcgc
     2041 ggcggcgggg taaaacagcg cgtttcagct ctggcgcaca ttgaacaatg gcggctctca
     2101 tttgcggttc aatgatgaag cttaatttat gagtcaagcg aacagcgcgg agaatgcgga
     2161 tcggatcttc aacaaaacga gtgactgagt caccaatcat acgcagtgta cgattttcaa
     2221 catctttgag cccgtcgcag taatcaagaa tcttgtgttg aataggatcg tagaataaag
     2281 cattgatagt aaaatcgcgt cttttcgcat cttcttcgca ggaaccgaaa taattatccc
     2341 caataacctc agagtcagca ccttccaact cttccgcact gatggctcgg cggaaggtcg
     2401 ccacttcaaa ttgttgatct ccgcggcgga cgagtactag acgaaaacgc ttaccaataa
     2461 cataggcttg agggattttc cggcgcacct gattggggtg agcgttcgtg gcaatgtcaa
     2521 agtctttggg atgaattcct gctaacaaat cgcggacgca gccgccgacc agataggtct
     2581 cgaaaccttc atcttggagg atgcgtacga tgtcgcgagc gtaggggtca atccagtctt
     2641 cgtgcaggtg gggttttgta gtcaatacgt actctcatgt ttgctcagct aatcagttgt
     2701 gacttttgtt gtaacggaat ggctagaccg tgtcaaattt tgcggtataa ttccgcctat
     2761 gttttatcta gatttatata attatcagat tatagggcct gaaaatggcc gtcgttgggt
     2821 ttttgttcat ggcctcatgg ggtatggtgc caattggcgt aaaattattt ctagtttgga
     2881 atctacagaa cgcgtcttat gctttgatca gcggggacat ggacgctcgc agaagcctgc
     2941 tgaaggctat actcctgaag attactccga agatatttta aaaatcactg aagagctagg
     3001 ctgggataaa tttatccttg tagggcattc catgggaggg cgaaatgtgc tgaacttcgc
     3061 ctatcgttat cctgaaaaaa tcatcaagct agtggtcgag gatattggac ctgaggcagc
     3121 accgaacgca gtagaatatt atgaaaagtt attagagatg gtgcctactc cgtttccgaa
     3181 tcgtgaagct gcacgaagtt tttttcagac ggaattcctt gaaaaagcta aaacacgaga
     3241 gaacccgcaa gtgctagcgc aatttttgta cgcgaatata gaagacaaag cggacgggac
     3301 tgtggattgg cgtttttata aacaaggaat tttggactca gtcagtgatg ggagaaacaa
     3361 agaccgctgg cacgaggtga tctctttgaa agtacccact ttatggattc acggggcgca
     3421 gtctcgtgaa ttcagccggg agaatttcga gagagtcttg gactccaatg agttgattac
     3481 tggcgtcgag atacctaatg cagggcattg ggttcactcc gaacaaccag ccttatttct
     3541 tcaagctttg aaggattttg cagggggctt ttaacttgct ccaactgcct gtttctttga
     3601 cttttcgtga agttctcatt actttggcct ttttaagacc taatactgtc tctttggagt
     3661 gaaaccttga atttttccga gtttaattta gatcccagcc tcaaatccgc catcgaaaag
     3721 ctcaactatg cagagtgcac tccaatccaa gaattgtcga ttccagtaac gcttgagggc
     3781 aaagatgtcg ccggcttggc acaaaccggg actggtaaaa cggcagcctt cttgcttccg
     3841 atcatggaaa gaattttgcg ggcacgtcct ttggcgccag gcgcaacaga agaaaccaag
     3901 gttctcgcag ccaagcgtgc tttttcagat tggaaaccac aaaattttgt tttaatttta
     3961 gtgcccactc gtgagctagc tgaacaggtt caggagagtg tagagaagtt ttctgagttt
     4021 tctggccttc gcggttttgc gatatatggt ggcactgggt acgataagca aaaagaggcg
     4081 cttaaaaatg gcgtggaatt tattgtggcc actccaggcc gcctgattga tctttacaaa
     4141 gaacacctcg ttgatttgaa gcaagtgcgc gcgattattt ttgatgaagc ggaccgcatg
     4201 tttgatatgg gcttcaaaga agatatgaaa tatattttgc aaagagttcc caaagaacgt
     4261 cagttcttgg ttttcagcgc gactttgaat tttgatgttt taaatacggc ttaccaattc
     4321 ggcgcagatc cggtggaaat caatattagc agggactctg ccaaggcaga aaatgtgaaa
     4381 gatgaaatct ttcatgtggg ccaagatgaa aaagggcagc atttgctatc gctcttaaag
     4441 ttgcacaagc ctcgtcaggc cgtcatcttt actaacttta aggtcaacgt tgagaagatc
     4501 actcgtttct taaacaataa cagtattcct gcaatggcga tttccagtct tttgactcaa
     4561 gctcagcgca ctaaagtgat gcagcaattc aaagcggaaa atgatttgaa cgtgctggtg
     4621 gctacagatg tggcggctcg gggtttggac gttcaaggcg ttgatatggt cgtcaatttt
     4681 gaacttccca tggacagtga atcttacgtt catcgtattg gacgtacagg ccgtgctggt
     4741 gcaactggat tggctttcag tttggtgagt gaccgtgatg tggactcttt gggtcgtatc
     4801 gaggagtacc tcaagcacaa agttgaagtc ggttatcttg aagacaaaga ccttttgaag
     4861 gatttcaaac cttttgtgtg gtcagattct tcttattctc gttcttatga atcccgcgat
     4921 ggcggcggtg ctggaggccg tcaagatcgt ggtccacgcc gtggagcggg tccgcgcact
     4981 ggtggcacag gaggtggagg acgtagtcct cgccgtgacg atcaacaacg tcgtgatcat
     5041 ggacctcgcc gcagcgacaa ccgcacaaca tcttctgcgg gaccacgccg tgagggagaa
     5101 cagcggcctc aacataaacg tggccctgag caccgttctg atcaacgtcg gcacacgcct
     5161 cataagcctg cggcccacca aggcaacggc caaggccaga gaccgcagca agcccagccg
     5221 catcgaggtc ctaagaaacc tcattcagcg caagtggcaa agcagaagtc tattggccaa
     5281 cgcgtgactg gttttatcaa aaaacttttc agctaagcct agagagctct agaggccatt
     5341 gtactcttat taactcaaac aaaacttcca ggccggaata acttggatct ctacaccttc
     5401 aagatgaagc tcttcagagt tagaccaagt tataattttg agatttgtta ctttccattt
     5461 tttagcagca tttaacagcg atgagagctc tctttttcga gttctttcat cgcttatatt
     5521 gaagcatact tgaataaggc tgcttatttt acgatcttcc gtgacggcaa agtctatgtc
     5581 gtactgactc gatttaagat aaaaaatatt ttttgttttt ctgcggaggt gcagaaaaac
     5641 aagcgtttca agtttcattc cgaaatcatc cgtggcttta gtgcttaggg aattcatcat
     5701 gcctatatcg caggcatagt ttttcttcgg taatgtagac cgctccttaa gtttatatga
     5761 gtaggctgag acctcataaa gaagatagga ctcctgcaag taggccagat gatttcgaac
     5821 cgtatttatc gaactgtaac caaaggcacg ctgcaattgt tgataactga tcactgagcc
     5881 cgattgctgt atcatataga ccccgagttc tttcaggagc ttcgggattc ttacattatg
     5941 cctctgtacg atatctcgac ctacgatctt gtcaaatagc tcgcgaaggt agagaccttg
     6001 tggctctcct tcaacaactt ctgggaatcc acctcgtgta aaatagtcct caaagtgtgc
     6061 agcacat
//
LOCUS       DATBTE010000319        31958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1067365, whole genome shotgun sequence.
ACCESSION   DATBTE010000319 DATBTE010000000
VERSION     DATBTE010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 31958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31958
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067365"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..502
                     /locus_tag="VIG33_11870"
     CDS             299..502
                     /locus_tag="VIG33_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271577.1"
                     /translation="MKITDKSLSCTSLQGDKVVFGKIDQARFVKDSTNGGTGAYHVTY
                     SSRVELKEFKTQNGNTEYQLIVK"
     gene            complement(495..1766)
                     /locus_tag="VIG33_11875"
     CDS             complement(495..1766)
                     /locus_tag="VIG33_11875"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HEY8271578.1"
                     /translation="MAKFYSFFIYPLAFILLHILRPFLNKKTQQMMEDKNSNFFEVQT
                     QSAQWIASKHPFWIHAASGEIEYARPIIRELKTRHPDVPIIVTYSSPSAKKILKGLTD
                     VDAWGMIPWDFEKDCLQFLEKWQPRCWLIARTDVWPVMATCCHRKQIPSLLFSATFAA
                     NSSRLKGLSAKVTRWALNQLSEIHCVSKEDVAELKRLSLQVPVRVHGDSRFDQVFYRL
                     EHPKPIKKELRPLPNAHVFVAGSTWPEDERVLLPAYAALKDHCKMILAPHEIHNDHLK
                     SLENQLQSLGLSFCRYSTATSWTEEEVLLIDQVGILAELYTWGTIAFVGGSFRKQVHS
                     VMEPLAAGLPVLVGPYHHNNREALLFKEKFIEGQKVVTEVLDKESLTTALLSKPTPLF
                     PQSLRHELMQHVHSSHNVVKWCGVQKRMPLT"
     gene            complement(1766..2788)
                     /locus_tag="VIG33_11880"
     CDS             complement(1766..2788)
                     /locus_tag="VIG33_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY8271579.1"
                     /translation="MAEKALFLIIRFSSFGDVTQSLSIPSAIQKAYPEAEIHWITRKD
                     MAPLLQHHPAIHRIWEYDRKTGLSGLWSLLNTMNEFPYTHIYDAHNNLRSRMVSWRLR
                     LFRSCKFIRRPQKRWKRFLLFQLHKNTYEMPFSGQRDLLEPLAAWNIAKTLPPTPQLF
                     VDHDDFSQANALLGDWSDKSYVALAPSAAYFLKRWPVSHFKQLILNMPDINFVLLGGP
                     EDRFIEELQKVAPERTLNTAGKASLTVSSALIKKSRVLISNDTGLLHVGEQLGKPVIA
                     LMGPAPFGFPSRPSTEIMELDLVCRPCSKHGQGPCINKTYHQCLVDITPEQVTQALRA
                     KLESSL"
     gene            2988..4328
                     /locus_tag="VIG33_11885"
     CDS             2988..4328
                     /locus_tag="VIG33_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271580.1"
                     /translation="MNSRMGQKILTIVGSLTICGLMNFGFQNCARTQFLVDPFAKSEA
                     LSKENVFGNQQGDDGSIAGTQQGDDGSIAGTQPGDDGSLPKGGGLPGKPPMGNDSSVP
                     GKTPPDKNPPLSNLSVNFIFECSNAHTSSVGGNLLTASAVKVVIAGRGNNFLCEVTGD
                     FKTEILNTKKLNFKPCAGLAPGKYDVHVVDEKVSANLFISKDFIRDKISFTVNADGTY
                     SLMAKPIRIIYDTNSSHSLYNQFNEQFGNASTAETQKRCDKRVSPLIVSMGSETRGIK
                     LTAPLDGIQFDILGQNSFPRAHDKKQISWFTPEVQEYYFIVLPGKDGKVLGIDQMFGD
                     NTKGPDGNFAANGYAALAKYDSDRDNLITDEDEVYGDLRLWLDSDRNGIAELNEIFTL
                     KEKGVILIDLHYDQNYKEVDPYGNQTLMKSVVKTEDGKLHLLFDLWFRYLNITK"
     gene            complement(4371..5450)
                     /locus_tag="VIG33_11890"
     CDS             complement(4371..5450)
                     /locus_tag="VIG33_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271581.1"
                     /translation="MMNGRILIYRVFDIGHDVNLEGAQKIFETTTTAQRFRLNRNSKA
                     MIINNAPLSLNVETSKYNALGHPLDLEISAKIWHFGAISITMSFEMPESMSWNRLIEL
                     GNFLENDNHLHTLATHKVEQILQSLDSTRIDQVNWETYEDYICYFFKKLEGCEKNAMN
                     IFERHDIFRLILSENNENLSEQIKKTILDSTFQYSNDDLAVIDWNSALIIEPSGSLDI
                     VDVIEFSLCQLLEMRHYDDLLDEKLTYLYSALEKKKYNIFQSYYNKLSKEAAKFYLDI
                     SDTVESVENSMKVVGDFYLAKIFRAASQRFRFKDWQDSVNKKLENLAQISKIFVSEGN
                     EKKNQLLEIIIIFLIAIEVIPLFFK"
     gene            complement(5469..7994)
                     /locus_tag="VIG33_11895"
     CDS             complement(5469..7994)
                     /locus_tag="VIG33_11895"
                     /inference="COORDINATES: protein motif:HMM:NF023454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3516 domain-containing protein"
                     /protein_id="HEY8271582.1"
                     /translation="MDWPLYWRLPPKAESTTDQILDRFLDYIASKNLELYPAQEEAIL
                     ALLDGKNVILNTPTGSGKSLVALALHFQSLARGRRSFYTCPIKALVNEKFRDLCADFG
                     PDQVGMITGDGSVNPQAPIICCTAEILSNLALREGPLANVDDVVMDEFHYYSDKQRGV
                     AWQIPLLTLPQTRFLLMSATLGEMDFFAKTLTDLNQQPTEVVRSTHRPVPLDFEYRET
                     PLHETVADLVTKGLAPVYLVSFSQREAAEETQNLLSVDFATKEEKKKIQEALQEVKFQ
                     SPYGKEIHKILRHGIGLHHAGLLPKYRALVEQLAQQGLLKVISGTDTLGVGVNVPIRT
                     VLFTKLCKYDGDKTGILSVREFHQIAGRAGRRGYDTRGTVVAQAPEHVIENLKLKRKA
                     ADDPKKMRKLVLKNPPPQGFVMWTKETFNRLLVSLPEPLVSRFQVNHSMLLNVLSRQN
                     GRRAMQNLIRKSHEKQSHKKALRQSAFELFRSLVDRKIVEYVPIQDESGARVQIRVNM
                     NLQTDFSLNQTLSLYLLDALQLLDPQSETYALDVLTLVESILESPELILRKQLDRIKT
                     EKMAEMKAAGMEYEERMEELEKLEPPKPNRDFIYSTFNAFREAHPWVGQENIRPKSVA
                     REMYENFQSFAEYIREYDLQRIEGLLLRYLSEVYKVLEQTVPETVKNDELKAMALYFG
                     SMVRQVDSSILDNWEKMRTSEEPMTKSPTTKDSVPAKPSMDRTALTLLVRNQLHSLVR
                     ALAQKDYEAVLSLIQPTWTTAEIEQMMKTFYQEHQRIRTDPAARHPQNTKIQYTDPHW
                     KVENTLLDEEDFNDWMMELSVDPVCLQEGSKPALHLLSIRPIG"
     gene            8166..8723
                     /locus_tag="VIG33_11900"
     CDS             8166..8723
                     /locus_tag="VIG33_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY8271583.1"
                     /translation="MRSLTPSRGRRELAPLSGMRNMMSRFDDVFNNLEREFFAEPAGG
                     RWAQTGLEGMTEFTPSVDIEESDEMYLISADLPGIKKEDVKIDVTGNTLRFSGERSKD
                     IKEEGYRERSYGRFSRSFTLPQNIDPKKVEAHFEDGVLRIVLPKSEVKSAQEIKIQSG
                     QPQGLIDRFLHREKNAKPTEEASKH"
     gene            complement(8919..10193)
                     /locus_tag="VIG33_11905"
     CDS             complement(8919..10193)
                     /locus_tag="VIG33_11905"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY8271584.1"
                     /translation="MRSHQRAIWLVGILTLTSQSFAQNNFEEVIAGAVKKVEEVQSKE
                     SELRSAELSRTQSQMRFLPDLNLSSTYTEEGPAWDQRNALRNYGLRSNLNLFRFGGDY
                     FYYKSTDLTMESLRWDLKNTKLTLEATISGKALDYIANHLETEIRKKITEAQQNFFNI
                     AEKRYAKGILSKQELDQITIDFKNAQAHLTDAQLTEFQAKEALKIYLGEGDIQATWPW
                     LDSLKKMPRKKVAFNVQSHPEWQLLQNKVQAFEYAQKNKFSEMLPSLDLSLSYTKENS
                     AYNNDPNASPWVTQWVSGITLTIPLFSRLENYTLYRQAIESKFRTDLELQRSSRELDA
                     QWKVSENDFHTQLASVLVREQTLKISNNLYQDNLRRFQAGRSTANDLLNDQERLYQSE
                     LLAIQGWKAVHLSYIKLCHAMGQLVTECGLKH"
     gene            complement(10190..13291)
                     /locus_tag="VIG33_11910"
     CDS             complement(10190..13291)
                     /locus_tag="VIG33_11910"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8271585.1"
                     /translation="MRLLYSSPLRVYLMLGVLSLVGIFSGLSLPISLFPNSSKPVVQA
                     SIPYGDTTAKDFLNSYGQSLESSLGSLNTKYVKVEKLEARYGKGNVTYTVSFPWETPP
                     NDALKEVETLVAQVTSSMPEEVRRGANVYTWSQNNGFFAASFYSPKRDLDELYKILDP
                     LFTAELKKVSDASNATLWNPSEKEIQIEINPDALTKYQLLPVDITRAVMSAMESYQGG
                     NLEIGDKRLQLIVKSAVVTIKDLQKVLITTPQGQTIHLTHVANIDYAVPLDRNRVIRT
                     SGALSVILFADPKSGGNIKKMSEDILQIIESKKHLLPTDIEYKIIVDPSEFIRSSVNN
                     VIHEVALAAGLAVLVLFLFIGSFKNVVTAAIEIPMSIVLAFILMKLTDMNLNLISLGG
                     LALSAGMNVDGSVVVMENIFRHFEKAKGVLDYKAKLNLLMDAVSEVKLPIIASTIASI
                     VVFAPLIFTKGLSSAILGDLAKAVVFSHGLSAIVALILVPTVRLQMMESETHFHPASP
                     LEKYFVRLENFYAKTLDLFIKNSLWRKSIYGGLVVSLLVLIQFVLPRLPKEIIGTPDT
                     DWVIFGMSTQGNTLVRQMETQSDEIEAKLLKFLNGQVLYTFTQINSPSNCNIMLRLKD
                     KKDMAAIIKSLENEFPATPTTQYWVDQWNPSELPLPDPENLLVVVRSQDTDKMLLATK
                     EILDGLQEKQYYHSLWAVPETNNPEGISVQPRLELWPEIAKAGSHVSPSDLADYSRVA
                     TEGRTIGYLTMNNQLTRIRLKYSTGFIKSTEELSALPLSVAGKLIPLKALAHINIEKT
                     RPSIYRLDQQEEYLLHGRLKKEEKSSSKLILKKAEAVVEDWKKTSPLAKEVSVSFDNP
                     EKEVDEAIHQLSWALGISTGLILVTMILQLGHVINSLLVFMSVPLGLIGVLLSLFIFR
                     STLSLNSMLGVILLNGIAVANSIILVDFLNRLVERGLTPHAAALEAARTRLRPILMTS
                     LTTTLGMLPIALGFGEGGRILQPLGIAVSGGLGVSMLLTLYVVPALQVSYLNWSEKKK
                     GKA"
     gene            complement(13288..14175)
                     /locus_tag="VIG33_11915"
     CDS             complement(13288..14175)
                     /locus_tag="VIG33_11915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY8271586.1"
                     /translation="MKKWSLVGFLCLLGQFTWAATDTAAKGAPEVVPIVQIKKITLTE
                     SSETFSYPARVESRVNSAILAEIEGVVREVATLGSSVKKGQVLFKIQQLDPVYQYAAA
                     KIVAPVNGVVSEVDVSLGTQVARGQKIAMVVDPSQLRVTVEIPGSDLEKVREKITPYS
                     SVSFSTALSEKPEELKFEGISPLVSSTTGTATANLLFKKKSLKLPAGTIGKVQITLNK
                     GSAIKIPEQAIVYKGATPYVRVIDTHNIAKYKAVELGSRQAGQVEIKKGLSVNELLIE
                     RSSQYVQDNKAVKVEKDKI"
     gene            14453..14752
                     /locus_tag="VIG33_11920"
     CDS             14453..14752
                     /locus_tag="VIG33_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271587.1"
                     /translation="MNRNLIQNNENCRNCPMVMGISQTIESEFNNWSLTTAEKEVCRA
                     LLMGKSLRKISQERHTNEMTVRQQAFAVYAKAGMSGRHEISAYFLKRFFQITSDL"
     gene            14785..16329
                     /locus_tag="VIG33_11925"
     CDS             14785..16329
                     /locus_tag="VIG33_11925"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8271588.1"
                     /translation="MGLRVRVIFVLCLLSFLLTGTQLLFTLEQIDSEKTSAIVTKSRA
                     ILSRLESVRNYIAEQGGLVWALENAKKNYPDGNLPKDVKLAILKQVPIFASMQVGAQE
                     AQKEMYQFRVFSDQPRSKENQATENELEVLKRFEADPNLKEIQSKTSESVIVYRPVRL
                     SEKQGCLNCHGNPENSPWGNGKDVLGFTMENWRDQKLHGVFAIVSDLNLAATREAKQK
                     QLLMILLTSGIITLSGLALAYWILSKPFKQVSVVAENLSASGNEVHLASAEIAKASLN
                     LNESAVEAANSLQETSVTMKFMSEKVSLNSKSAQMAYQLSAQSKESAEKGFQEFSVLV
                     SSIENIKASSSKIEQIIHLIDEISFQTNLLALNAAVEAARAGEQGKGFAVVADAVRTL
                     AQRSASSAREISELIKESVERIDQGNEIALQSRTSLQTIVESVEKAAALNKEIADANE
                     EQAREIINLNTTMSELDAVTKSNAKNAEQTSAASEELSAQAQQLQKFVLDLNRVIDGR
                     KEEADL"
     gene            complement(16324..17112)
                     /locus_tag="VIG33_11930"
     CDS             complement(16324..17112)
                     /locus_tag="VIG33_11930"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY8271589.1"
                     /translation="MKNCMNLLVLLFAFTMQIRAQAQTQEPTLVDSTTAAGLVTKTPK
                     PEPIKPLLTFEPVGGITFSNFTGGSGPYKFESRTGYQGGLGFLIGRGKFQFETGLLYA
                     ERGSREIYQLGISHWQIDYNNRYIEVPTLIRYNYELSRDAKIFLKAGAVVAVLQDSTG
                     PISNTQNYTAMGNYYGMYYNSSGVAINDGNTKNYFGSTDIRWAAGIGGGVKITKSISW
                     TMQMDYQTSITKVSENQPDGYLGTTNMNLFAVTYGLNTGIAFSL"
     gene            complement(17325..17960)
                     /locus_tag="VIG33_11935"
     CDS             complement(17325..17960)
                     /locus_tag="VIG33_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY8271590.1"
                     /translation="MEQLLLPLAIIASSFLGSWHCAGMCGPFAALTGSRGQLWHYHLG
                     RLIIYFSLGTASGFFGEFVLKSEFRWLRATGAFLLGITLIVMGLQYLLSANIWQNSHH
                     WAGKLFQRLYLKIKIFKFSRSSFVIGLMAGLLPCMWLYTYVLAASATKSPWAGGFTMI
                     LFWLGGLPALSAVSMMIRPALQKSNERKQKIAGGVLVCAGLYALVSHLLSA"
     gene            complement(17962..19365)
                     /gene="ccoG"
                     /locus_tag="VIG33_11940"
     CDS             complement(17962..19365)
                     /gene="ccoG"
                     /locus_tag="VIG33_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEY8271591.1"
                     /translation="MALDPERLTSIDEFGDRTYIIPAEVRGFYHRYKNWIHIILLVIF
                     LGLPWTSINGTQTILLNISARKFAIFGVTFWAHDAPLVFFILATLTLGLAFVTSIWGR
                     VWCGWACPQTVFIDSVFRRIERWIQGTYIQRRRLKQAPLSFSKIFKTLLTWFLFFVVS
                     SLIAHSFAAYFVGSEAMIKMLQESPGQNWETFLIVSFITLVVLFDFGWFREQFCIIMC
                     PYGRFQSVLLDQNSLAVVYDQARGEPRKGKPSMNEKQGDCVNCNRCVQVCPTGIDIRK
                     GLQMECISCAACADACDEIMVKVKKPTGLIRYDTLDHSKLTLTKPRSLVYMALILLSL
                     IGLTYNISTREKAQVFILRAKEAPYTLIKNDKGENMILNHFRLEIQNQNFKKAIYLLR
                     PPTSWSQQGIELTVGQNPLNVEPGKMIEWHFFIRFKPELVAKTGQLKTQILIEDQSEV
                     ESPFKSEKEFTLIGPKQ"
     gene            complement(19388..19966)
                     /locus_tag="VIG33_11945"
     CDS             complement(19388..19966)
                     /locus_tag="VIG33_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8271592.1"
                     /translation="MSDIKDSINDPLTDHDYDGIQEYDNPLPTWWLATFQITIIFAFI
                     YFIHYQFGGGLTLAQELDIAMKSIETTTAQHAPAETESEDMLTAAIKNPKAVEVGAAA
                     FSTKCASCHGPQLGGLIGPNLTDNFWIHGKGTPMDIVTVIRKGVADKGMPPWETLLSK
                     EEVYGMTAFILSKKGSNPANPKAPQGVEITSK"
     gene            complement(19969..20133)
                     /locus_tag="VIG33_11950"
     CDS             complement(19969..20133)
                     /locus_tag="VIG33_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HEY8271593.1"
                     /translation="MTKEAFSYFTDTQVTSLGLIIFFVFFMGVLCWTSLKANKAIYKD
                     LEQLPLKEGN"
     gene            complement(20130..22289)
                     /gene="ccoN"
                     /locus_tag="VIG33_11955"
     CDS             complement(20130..22289)
                     /gene="ccoN"
                     /locus_tag="VIG33_11955"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEY8271594.1"
                     /translation="MKSPELNPHNLRDNLTEKIFYDDDIVKKFILATLIWGGAALLFG
                     LIAAIQLAWWPLNMNMEWVSFGRLRPLHTNAAIFAFAGNAIFAGIYHSTQRLLKTRLF
                     SDLLGRIHFWGWQLIILAAAITLPLGYTQSKEYAELEWPIDIAIAGIWVVFAVNFFVT
                     LSRRRERHLYVALWFYIATIITVAVLHIVNSIEIPVSFLQSYPVYAGVQDALVQWWYG
                     HNAVAFFLTTPFLGLMYYYVPKAANRPVYSYQLSIIHFWSLVFIYIWAGPHHLLYTAL
                     PEWAQTLGMIFSVMLWAPSWGGMINGLLTLRGAWHLLRTDPIIKFFVMAITFYGMSTF
                     EGPLLSIKSVSALAHYTDWIVGHVHAGALGWNGMMIFGMLYYLVPRLWKTKLYSEKLA
                     TNHFWIATLGILLYYISMVTAGITEGLMWRAISPEGRLVYPDFIETVIRIVPLYWVRA
                     FGGLLFIAGYFMAAYNLYKTIQSAPKAQEEVAVQVPNRASVFSRMDAAVGHGKLEGMA
                     TVFSVLAFLAIVVGSVIEIYPTLSLHKYITPNNMVEPYTPLELAGRDLYLKEGCYVCH
                     SQQIRPIASEVLRYGKASTLEESMYDRPFQWGSKRIGPDLARVGKKYPNLWHFQHMLD
                     PRAVTPKSIMPNYMWLTEKDTDFLILRKKLSVMKMLGVPYDDNTVANADIEAQKQAMV
                     VAEDLKANGINIPKLEQKQIIAMIAYLQSLGQKGKTP"
     gene            complement(22261..22443)
                     /gene="ccoS"
                     /locus_tag="VIG33_11960"
     CDS             complement(22261..22443)
                     /gene="ccoS"
                     /locus_tag="VIG33_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEY8271595.1"
                     /translation="MNILILMIPMALLLGFGFVVAFLWSTSQGQFDDVETPAHRILQD
                     DPQERNCREKPRTQPS"
     gene            complement(22440..24668)
                     /locus_tag="VIG33_11965"
     CDS             complement(22440..24668)
                     /locus_tag="VIG33_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEY8271596.1"
                     /translation="MDIVLPLSECLYCKTLLSPPQPSFCCAACEALYKLENSHDKGIK
                     KPSFSLSLEKYKFLDEQLSAHNTCEVTFHAEGLHCSSCVHLLERLPEFDSNILESRVH
                     FGESTVHVRVKPNTSWAQVAALIEELGYAPTAIVSSEKLLEKQKQENRKSLMRIGVAG
                     ACAGNMMLFVVPVYAGLAGTLAELFNWISFALFLPILFYSAWPFYQGAWNFLKYRAVN
                     VDLPIAVAMLSGFILSTYNLVFRVGGPVYYDSTASFIFLILSSRYLLKRVQQKHLSGD
                     YLLEGLQNHRHLTPGAQITVLQGEKIPADGVLLSSQAEVDMSLFNGESLPQSFREGMS
                     LFAGSTLLSPKILLLVEKIALETRLGKILQQLRSDSVQKSHFVSLTDKLAQGLIVTVS
                     SIALLFFIFYSAVDATAALERALALIVIACPCALAFGTPLAYGLSLRKAQKQGILIRN
                     SDIFEKLQHIENIFFDKTGTLTEGRLKLALIDGDLSEEDRKIILALETKSSHTIAFAL
                     RHAWSSLQHLPEVEDHQEILGEGVSGLVHNTRYSLTRSQELNILDYIAVEYRIEGRLM
                     AHLFFEDPLRLESKEILGKLAQFQLHILSGDKSPRVTKIASLCGIPEARCHAELSPEN
                     KLKEIQAAKHTCMIGDGANDSLALKAADVGIAVKGSVDLSLMSADVYFTRGGLQPLLE
                     LMQISKEARRTVRRNLVISLFYNGLGGALALGGFINPMMAAILMPISSVLIIISSLRG
                     TR"
     gene            complement(24759..25457)
                     /locus_tag="VIG33_11970"
     CDS             complement(24759..25457)
                     /locus_tag="VIG33_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY8271597.1"
                     /translation="MSSTKKTDDDHDSPNCALCTANPQNIICASSPHIPKYLERSKIS
                     CSYKRDQVIFYADNDPLGLFFVKSGLVKLEKISEDGSAHTLRIVGPGGVLGYRSLFAD
                     EPYHATAIAVDQLELCFVPKAEILLTFQNHPEVALRLLNHLAKDLRLAEERWIHQVDK
                     EAGERIAEALIFLQDHFQSQTWTRREIAEWAGTTPETVMRTLAQFEKEALLVQNGRQI
                     TILNKELLKSKANL"
     gene            complement(25509..26471)
                     /locus_tag="VIG33_11975"
     CDS             complement(25509..26471)
                     /locus_tag="VIG33_11975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY8271598.1"
                     /translation="MSPNSHHAHSHSHAHSHGQSHAHSHAAPRNFDKAFAIGIFLNLI
                     FVIAEAGYGLVSKSLALVADASHNFSDVVGLVLAWAALWLSRKKPSARFTYGLRRSSI
                     LAALTNAVLLLIAVGGITWEALRRLWEPTPIQTETVMVVACVGILINGVTAYFFMSGR
                     KEDINIRGAYLHMATDALVSVGVVVAGFLIQQTQWLWVDPAISIFIALIITVGTWDLL
                     KESVNMAMDAVPAGIHLIRVREYFTGIQGVQEVHDLHVWPMSTTETALTVHLVMPKEL
                     QTDELLAKISKDLKENFKIDHPTIQIESGDSTHFECALKPDEVV"
     gene            26580..27974
                     /locus_tag="VIG33_11980"
     CDS             26580..27974
                     /locus_tag="VIG33_11980"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEY8271599.1"
                     /translation="MKSHVVIIGGGLSGVLVSLELARLPQGPEVTLIEKNPEHLGRGV
                     AYDSHFTHQPLNVIAGGMSLFPDKPMDFVEWLKKNHFKYKHLVEQVSSQEFIPRKIFG
                     DYVLENLQRVQRQAGGRLQIRIGEAISIVDFGERKKVILHSGHELLADQVILALGNFP
                     PGDLFPEEDSLQKNPHYYANPWSDKVYSHIEGNENLLLVGAGLTAVDIVLGLILRKFK
                     GKVTLLSRRGRWPLSHDLSHPVFQFAEPEIQHPRKMLLWLRQLIRKNPQVPWPSVMDG
                     LRPFTKKIWIKWSIDEKKYFLKRLKPYWEIARHRIPTKSIFQLKEMKNLGQLELKKGC
                     LLNVQAAENGIEVVYRSQNKECRQIFQKVINCTGPELNYRKVHCPLIHDLLKRGKIKT
                     DELGLGLECTPEGQILNVQGKIEQGFWCIGPMRKAVLWETTALCELREQASELVSLIY
                     KKGLSPCSSLEQSE"
     gene            complement(27940..29007)
                     /locus_tag="VIG33_11985"
     CDS             complement(27940..29007)
                     /locus_tag="VIG33_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEY8271600.1"
                     /translation="MFRFIGLFLLIGFFLFNTSSQSFAAEADPCPSVDFRERFGPLKN
                     QGFHQICWAMAGSSLIEEEACLMDPKYCGVNFSAVDLTSCDPRVGHNHEENVVDRALS
                     CAQIQGVCPNHLAPFVDFRMKPSFLGRLTMSKRFQQSKLFKLFDEYKYALNHKSQAKH
                     SSAQVQSLQTNLLKEFRQTFSHNDFTDAQLTTALKKSKDAVELLDRTLISNACKQHRV
                     KFPGIGTVFTRIFPDLNPRILDYYTSQEIQNFSLPVSTQMKVIVERFKTGRSLALSIC
                     PEKFSLIENMMIPGGDCVAGHAIVANALRRNPETQVCEVHLMDSRKEASTIQFNGWFN
                     LEEVANATIAITQIVPKKNKD"
     gene            complement(29018..30100)
                     /locus_tag="VIG33_11990"
     CDS             complement(29018..30100)
                     /locus_tag="VIG33_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271601.1"
                     /translation="MKKTMTFIGLLFLFPFMYKALNPTPTPNSTPNPQEVAPPKTQGL
                     TGDPTYISIVNDCGALNERDFAEFNITEAPLESELRSQAKLEFISTKLQGAESAEAAR
                     EFNRTRATQQTYAGKIIKKSACSNKDHKFYGVRNLKSVLNGLVFRGGANNVYNHEFGF
                     KENENPLPEIALKNLCIRGFSNAVYLYSKNFKGPSMVNCKTQANENNTLHYIRLQPER
                     AFQTKSQTEDLETLIKLTHEQIMNAENKQRPIYVHCYNGWHASGRAAAFLLQQFCNAD
                     PATALSYWIENAHNSYEAPYRAIWKQIVKFDQLKNSAPFSHYKIDDRLKEKVCPDLKN
                     YSELAQKNNEVKTFLNKFSTASEGNH"
     gene            30567..30911
                     /locus_tag="VIG33_11995"
     CDS             30567..30911
                     /locus_tag="VIG33_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271602.1"
                     /translation="MKLVGNGKEVRRCTTIDGASFIVSEFRKPKKLEEWQLGILELPV
                     KSYKADDAPILVLQNNGTARSLSSTSHLIGSVTDIVEKMYFLRIDSNINETQLIKKLE
                     NKHYLMASEPQN"
     gene            complement(30978..31274)
                     /locus_tag="VIG33_12000"
     CDS             complement(30978..31274)
                     /locus_tag="VIG33_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8271603.1"
                     /translation="MANKKTTTSFKSTEDLARSLGFTKAEAEIVTMKGTVIGQLDKER
                     ERRGLNNTEFAEFLNIPKSRWSSIFSSPEKVTLDYLLTLAAKCGTLFKLTRKVA"
     gene            complement(31267..31596)
                     /locus_tag="VIG33_12005"
     CDS             complement(31267..31596)
                     /locus_tag="VIG33_12005"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY8271604.1"
                     /translation="MGLHLSKQVLKDIQGETKEIQEKLLILFRALEEGASLTMPVSRQ
                     MSGYPGLKELRVRDSRGIVRVFYYVQQKDHVFILHLFRKKTQKTPPQEIEIAIQRLKR
                     MKEEFHG"
BASE COUNT         8951 a         6909 c         7551 g         8547 t
ORIGIN      
        1 gagaaatacg tgttggcgta ttgagacaaa gcattgccct gagcaatcct cccgatggtt
       61 gccgctggaa acaaccccta gatcatcgtt tgacgcctat gaaaatttaa ttttttaccg
      121 gacagcagtg agaattttga actggggccg ggacttgaga aatgtctttg attattgcga
      181 actcaatgag cttgaaggct ggggatttgt ttcgcgaaaa aagaagtcta gtaagcggca
      241 agaaagcagt taagaactgc tcccaaggct tctgaatctc tccccccttg cagcgacttt
      301 gaaaattact gataaatccc taagctgtac ttctttacaa ggagacaaag ttgtatttgg
      361 caaaatagat caagctagat tcgttaaaga ctcaacaaat ggaggcactg gtgcctacca
      421 tgtcacctat tcttcacgtg tggagttgaa agagtttaaa actcagaatg gaaacaccga
      481 gtaccagctc atcgtcaagt aagcggcatt cgtttttgca ccccgcacca cttcaccaca
      541 ttgtgactgg agtggacatg ctgcataagt tcatggcgga gggattgagg aaatagaggg
      601 gttggctttg acaaaagcgc tgtggtcagg ctttctttgt cgagaacttc agttaccact
      661 ttttggcctt caatgaattt ttctttaaac agcaaagcct cacgattatt atggtgataa
      721 ggtcctacca agactggaag tcccgcggcg aggggttcca tcacgctgtg aacttgtttg
      781 cgaaaagagc cgccaacgaa ggcgatagtt ccccaagtat aaagctctgc aagaataccc
      841 acttgatcga taagcaggac ctcttcctca gtccaagagg ttgcagtgga gtatctgcag
      901 aagctaagac ccagactctg gagttgattt tccagagact ttaggtggtc attatgtatt
      961 tcatggggag ctaaaatcat tttacaatga tcttttaaag cagcgtaagc gggaagaagg
     1021 actctttcat cttcaggcca cgtagagccg gccacgaaaa catgggcgtt tggcaacggg
     1081 cgaagttctt tcttgatggg cttaggatgt tccagacgat aaaagacttg atcaaatcgg
     1141 ctatccccat gaacccgaac cggcacttgc aaagaaagtc gtttaagttc agccacatct
     1201 tctttggaaa cacagtgaat ctcggaaagc tgattcagcg cccagcgagt gaccttggcg
     1261 gaaagacctt tcaggcgcga gctgttggcg gcaaaagtcg cagaaaataa aagacttgga
     1321 atttgttttc tgtggcagca agtggccatc accggccata catccgtgcg ggcaatcaac
     1381 caacagcgcg gttgccattt ttctaaaaac tgcaggcaat ccttttcaaa atcccaggga
     1441 atcattcccc aagcatccac atcggtcagc cctttgagga ttttttttgc ggaaggcgaa
     1501 gagtaggtca caataatggg aacgtcaggg tgtctggttt tgagttcgcg gatgatgggg
     1561 cgagcgtatt ctatctcgcc actggcggca tgaatccaga aagggtgttt tgaggcgatc
     1621 cattgagcac tttgagtttg cacttcaaag aagttggagt ttttgtcttc catcatctgc
     1681 tgagtctttt tgtttaagaa aggtctaaga atatgcagga gtatgaaagc caaaggataa
     1741 ataaaaaaac tataaaactt cgccattaca aagagctttc caatttggcg cgaagtgctt
     1801 gggtcacctg ctcaggagtg atatccacaa ggcattggtg ataggtctta tttatacaag
     1861 gtccttggcc atgtttactg caaggacgac agactaagtc taattccata atttccgtac
     1921 tcggacgtga gggaaaacca aagggagcgg ggcccatcaa ggcgataaca ggtttaccaa
     1981 gctgctcgcc gacatgaaga agaccggtgt cattactaat gagaactcga gatttcttga
     2041 tgagggcgga actcaccgtc agagaagctt ttccggctgt gttgagggtt ctttccggag
     2101 ccactttttg cagctcctca ataaagcgat cttcggggcc tcctaaaagg acgaaattta
     2161 tatccggcat gttcagaata agctgtttaa aatgactcac gggccagcgc tttaaaaaat
     2221 aagctgctga aggcgctagg gccacatagc ttttatcgct ccaatccccg agcagagcgt
     2281 tggcttgaga gaaatcgtcg tgatccacaa aaagctgagg tgtgggagga agagtcttgg
     2341 ctatattcca agccgctaaa ggctccagca aatctcgttg ccctgaaaaa ggcatttcgt
     2401 aagtattttt gtgaagctgg aaaagcaaaa aacgcttcca acgcttttgc ggcctccgaa
     2461 tgaacttgca agagcggaaa agacggagtc tccagcttac catgcgcgag cgcaagttat
     2521 tgtgagcatc gtaaatatgg gtataaggaa actcattcat agtatttaac aaactccata
     2581 gaccagagag acccgttttg cggtcatatt cccaaatgcg atggatcgca ggatgatgct
     2641 gaagcaaagg ggccatgtct ttccgagtga tccagtggat ttcagcctcc gggtaggcct
     2701 tttgaatggc cgacggaata ctgaggcttt gagtgacatc cccaaaacta gagaaacgaa
     2761 tgattaaaaa aagagctttt tcggccatat gagcaggact ctagcaaaga agccggcatc
     2821 tggcaaaatg agaagggttt caaagtgaga aatgtcgaga gttttcacga cagctgtaaa
     2881 aaaagcagac tgagtttcag ctcaaaagag aactgaataa gtttaggttg gccttattta
     2941 tgcagcacaa ggaatgaaga ggttttaatt ttttggagga gacgtcaatg aactcaagga
     3001 tgggtcagaa gatattaacg attgttggga gcctaacaat ctgtgggtta atgaattttg
     3061 gatttcagaa ctgcgcaaga actcaattct tggtagatcc cttcgctaaa tcagaagccc
     3121 tcagtaaaga aaatgttttt ggcaatcaac aaggtgacga tggcagcata gctggaacac
     3181 agcaaggtga cgatggcagc atagctggaa cacaacccgg tgacgatggt tccctgccta
     3241 aggggggcgg ccttcctgga aaacctccta tgggcaacga ctcttctgtt ccagggaaaa
     3301 cacctccgga taagaatcct cctttatcaa atctttcagt gaacttcatc tttgagtgct
     3361 caaatgccca tacttcgtct gtggggggga atcttttgac agcttcagcc gttaaagtcg
     3421 tgattgcagg cagaggtaat aacttcctct gtgaagtcac cggagatttt aagacggaaa
     3481 tactgaatac gaaaaaactg aacttcaaac cctgtgctgg gcttgcgccg ggaaaatatg
     3541 atgttcatgt agtcgatgaa aaagtttcag ccaatttgtt catatccaaa gattttatcc
     3601 gggataagat ttctttcacc gtcaatgcgg acggcactta tagcctcatg gcaaagccga
     3661 taagaattat ttatgacaca aatagtagtc acagtctcta taaccaattc aatgagcaat
     3721 ttggcaatgc cagcactgct gagactcaga aaagatgcga caagcgagtg tcccctctga
     3781 ttgtcagcat gggttcggaa accagaggta ttaaactgac cgcgcccctt gacggcattc
     3841 aatttgatat cttggggcag aattctttcc cgagggctca cgataaaaaa caaatttcat
     3901 ggttcactcc tgaggtccaa gagtattatt ttatagttct ccctggcaaa gacggaaaag
     3961 ttttagggat tgatcaaatg tttggcgata atacaaaagg gccggatgga aactttgcag
     4021 ccaatggata cgcagcgctg gcgaagtacg atagtgatag agataactta atcacggacg
     4081 aagacgaagt ttacggggat cttcgccttt ggttggacag cgatcgcaac ggcattgcgg
     4141 agcttaatga gattttcact ctcaaggaaa agggcgtgat ccttattgat ttgcattacg
     4201 accagaatta caaagaagtg gatccttatg ggaatcagac tcttatgaag tcagtggtaa
     4261 aaacagaaga tggtaaactc catctgttgt ttgatctctg gttccgttat ctcaacatca
     4321 caaaataaaa gggggtgccc tgggtaatac cagggcccgc ttttgaatgc ctatttaaag
     4381 aagaggggaa taacctctat ggcgatcaaa aaaataatga ttatctctaa aagttggttc
     4441 tttttttcat ttccttcaga gacgaaaatt tttgagatct gcgctagatt ctcaagcttc
     4501 ttgttcacgc tgtcttgcca gtctttaaaa cgaaaacgct gcgaagccgc gcggaaaatt
     4561 ttcgccaggt aaaaatctcc aacgactttc atgctgtttt ccacgctctc gacggtgtct
     4621 gagatatcca gataaaattt agcggcttct ttggaaagct tattgtaata actttgaaag
     4681 atattgtatt tctttttctc tagcgctgag taaagataag tcagcttttc atctaaaaga
     4741 tcgtcatagt gacgcatttc cagcaactga caaagggaga attcaatgac atccacgata
     4801 tccaaagaac cagaaggttc gatgatcaag gctgaattcc agtcgatcac cgccaggtca
     4861 tcattgctgt attgaaaagt gctgtccaaa atggtttttt tgatttgttc tgacagattt
     4921 tcattatttt cggataaaat caggcgaaag atatcatggc gctcaaagat gttcatggca
     4981 ttcttttcac agccttccaa ctttttgaag aaatagcaga tgtaatcttc ataggtttcc
     5041 caattcactt ggtcaatccg tgttgaatcc agactttgca ggatttgttc cactttgtgg
     5101 gtggccagcg tatgcagatg attgtcattt tcaaggaagt ttccaagttc gatcaaacgg
     5161 ttccaagaca tgctctccgg catttcaaaa gacatggtga tggaaatagc cccaaagtgc
     5221 caaattttcg ccgaaatctc gagatccagc ggatgtccga gggcgttgta cttcgaagtt
     5281 tcaacattca gagataaagg agcattatta atgatcatgg ccttggaatt tcgatttaat
     5341 cgaaacctct gagccgtggt ggtcgtttcg aaaatttttt gagcgccctc gagattcaca
     5401 tcgtggccaa tatcaaaaac tctgtaaata agaatgcgtc cgttcatcat aggggatggt
     5461 ttcctttttt acccgatggg gcgaatcgag agaagatgga gcgccggttt tgaaccttcc
     5521 tgtaaacaga caggatctac actcaactcc atcatccaat cattaaagtc ttcttcatca
     5581 agcagtgtat tttccacttt ccaatgaggg tctgtatatt gaattttggt gttttgtggg
     5641 tggcgagcgg cgggatcagt tctgatcctt tgatgctctt ggtagaacgt tttcatcatt
     5701 tgttctattt ccgcagttgt ccacgtcggt tgaataagag acagcacggc ttcgtaatcc
     5761 ttttgcgcga gggctctgac caagctgtgc aattggttac gcactaataa agtgagggct
     5821 gttctgtcca ttgaaggttt tgcgggaacc gagtcctttg tcgttgggga ttttgtcatg
     5881 ggttcttctg aagtccgcat tttttcccaa ttatcgagaa tgctggaatc cacttggcgc
     5941 accatagagc caaagtaaag ggccatggct ttaagttcat cattttttac tgtttccgga
     6001 acagtttgct ccaaaacctt ataaacctct gacaaatatc ttaaaagtaa gccttctatt
     6061 cgttgcaagt cgtattctcg gatgtactcc gcgaaagatt gaaagttttc atacatttca
     6121 cgagccacgg acttcggacg aatattttct tgacccaccc aaggatgcgc ttcacggaag
     6181 gcgttaaagg tggagtaaat aaaatcccgg ttgggtttgg ggggctctag tttttcaagc
     6241 tcctccatgc gttcttcgta ctccatacca gcggctttca tctcggccat tttttcggtc
     6301 ttaatccgat ccagctgttt gcgtaaaatc aactcgggac tttctaagat agactccacg
     6361 agggtcagga catccaaagc gtatgtttca gactgaggat ctagcaactg tagagcgtct
     6421 agcaaataca aagaaagggt ctgatttaaa gagaaatcgg tctgtaaatt catattgacg
     6481 cgaatttgca cgcgggctcc gctctcatcc tggatcggaa catattccac aattttacga
     6541 tccactaaag atctaaaaag ttcaaaagcc gactggcgga gggctttctt atggctctgt
     6601 ttttcatggg attttcgaat gagattctgc atagctctgc ggccgttttg ccgactgagg
     6661 acattgagta acatgctgtg attcacttga aatcttgaga ccaaaggctc gggtaaagac
     6721 accagtaaac gattaaaagt ctctttagtc cacatgacaa agccctgagg gggaggattt
     6781 tttaaaacca gcttacgcat ttttttagga tcatcggccg ctttgcgctt caattttaaa
     6841 ttttcaatga cgtgttctgg agcttgcgcc accactgttc cgcgggtgtc ataaccgcgg
     6901 cggccagctc gccccgcgat ttgatgaaat tcgcggacgc tgaggattcc ggttttatct
     6961 ccatcatact tgcaaagttt ggtgaagaga acggtgcgaa tcgggacatt gactccgacg
     7021 ccgagagtgt cggttccgct aatgactttg agcaaacctt gttgggctaa ttgttcaacc
     7081 aaggcccggt actttggcaa gagtccggca tgatgcaaac ctatcccatg gcgcaaaatt
     7141 ttatgaattt ctttgccata gggactttgg aatttcacct cttgcaaggc ttcttgaatt
     7201 ttctttttct cttctttcgt ggcaaaatcg acactcagga gattttgtgt ttcttcagcc
     7261 gcttcccttt gtgaaaaact gactaaataa actggagcca gtccttttgt caccaaatcc
     7321 gcaacagttt catgcagagg tgtttctcga tattcaaaat ccaaagggac tggccggtgg
     7381 gtggaacgaa ccacttccgt gggctgctgg tttaagtcgg tcagggtttt tgcgaaaaaa
     7441 tccatttctc ctaaagttgc agacattaat aaaaaacgag tttgaggcaa ggtgagcaga
     7501 ggtatttgcc aggccacgcc acgctgcttg tctgaataat aatgaaattc atccatcacc
     7561 acatcatcca cattggccaa aggtccctcg cgaagagcca gattggaaag aatctcagca
     7621 gtacaacaaa taatgggtgc ttgcgggttg accgacccat ccccggtgat catccctact
     7681 tgatccgggc caaaatcagc acaaagatcg cgaaactttt cattgaccag ggccttgatg
     7741 ggacacgtat aaaaggatct gcgaccgcga gcgagggatt gaaagtgcaa ggctaaggca
     7801 accaaagatt ttccagaacc tgtcggagta tttaatataa catttttgcc atctagcagg
     7861 gctaagatgg cctcttcttg agccggatac aattcaagat tcttagaagc gatataatct
     7921 aaaaaacgat ctaatatttg atctgtagtg ctttccgcct taggcgggag cctccaatag
     7981 agtggccaat ccattgaatg actctactcg ggcttttcac acttcacaag gagttatggt
     8041 ttatgtcaaa aacaaatagt atcgccttaa atttctttga tttttcaagg gatccctatt
     8101 tctttttctt aggactattg caaagtaaag cctccaaatt attaactcgc tagaggaggt
     8161 tttttatgcg ttcactaact ccatcacgtg gccgtcgtga acttgcccca ttgtctggga
     8221 tgagaaatat gatgtcccgt ttcgatgatg tctttaataa tcttgaaaga gaattttttg
     8281 ccgaaccagc aggggggcgc tgggcccaga caggactgga aggaatgacg gaattcacgc
     8341 cttcggttga tatcgaggaa agcgatgaga tgtatttgat ttcagccgat ctgccaggga
     8401 ttaaaaaaga ggatgtcaag attgacgtca ccggcaacac gctgagattt tccggagagc
     8461 ggagcaagga tataaaagaa gaagggtatc gtgaaagatc ctatggacgc ttctctcgca
     8521 gctttacttt gccacaaaat attgatccaa aaaaagtcga ggcccatttt gaggacgggg
     8581 ttctcagaat cgttttgccg aagtcggaag tgaaaagtgc tcaggagatt aagattcaat
     8641 caggtcagcc tcaaggcctt attgatcgtt tcttacatcg tgaaaaaaat gcgaagccga
     8701 cggaagaggc cagcaaacac taggtcttaa taaaaacagc tcctggtcgg agctgttttt
     8761 caaacaagca gcatctctaa taattttttt tcacgcgcat ccgtcgcccg gagatcaatg
     8821 cgtcgccagt tccgctcata gagcaatccg cttcaattgc ttataatcac tgatgcaaat
     8881 cttcttagga agtttttgct cagatgatcc tttctggatt agtgtttcaa accgcattcg
     8941 gtcacaagtt ggcccatggc gtgacaaagt tttatatagg ataaatgcac ggccttccag
     9001 ccttgaatag ccagaagctc ggattgataa agacgttctt ggtcattgag taaatcattg
     9061 gcggtgctgc ggcccgcttg aaaacgacga agattgtcct gatagagatt atttgaaatt
     9121 ttcagggttt gctcgcggac caggaccgag gcgagttgag tatggaaatc attttcagac
     9181 actttccatt gagcgtcgag ttcccgtgaa gatctttgta attccaaatc cgtgcgaaat
     9241 ttgctttcga tcgcctggcg ataaagagtg tagttttcaa gacggctaaa aagagggatg
     9301 gtcaaggtga tgccgctcac ccattgagtg acccaaggac tggcattcgg atcattgttg
     9361 taggctgagt tttctttggt atagctgagg ctcaaatcca gggacggtag catttctgag
     9421 aatttatttt tttgtgcgta ttcaaaagct tgtactttat tttgcaaaag ctgccactca
     9481 ggatgactct gcacattgaa tgcaaccttc ttccgaggca tttttttcag agaatccagc
     9541 caaggccaag tagcctggat atcgccctcg cccagataaa tttttagggc ctctttagct
     9601 tggaattccg tcaattgggc atcagtcaaa tgcgcttgtg catttttaaa gtctatggtg
     9661 atttgatcga gctcttgttt tgaaagaatg ccctttgcat aacgtttttc cgcaatatta
     9721 aaaaagtttt gctgagcttc agtgattttt ttacggattt cagtttctaa atggttcgcg
     9781 atataatcca aagcctttcc tgaaatggtg gcttccaggg tgagctttgt gttttttaaa
     9841 tcccaacgca agctttccat ggtgagatcc gtcgacttat aatagaaata atctccgcca
     9901 aaacggaata aattcaagtt cgagcgcagg ccataatttc ggagggcgtt acgctgatcc
     9961 caggctggcc cttcttcagt ataagtgctg ctgagattca agtccggtaa aaaacgcatc
    10021 tgcgactggg tcctagaaag ttccgcggaa cgaagttcag actctttgga ttgaacttct
    10081 tcgacttttt taaccgctcc agcgatcact tcttcgaagt tattttgggc aaagctttga
    10141 gaggtcagtg tcaaaattcc gactaaccag atagcccttt gatgagatct catgctttcc
    10201 ctttcttttt ctctgaccaa ttcaaataac tcacttgaag ggcgggaacc acataaagag
    10261 taagaagcat tgaaaccccc aagcctccac tcaccgcgat ccccagaggc tgaagaatac
    10321 gtcccccttc accaaagccc aaagcgatgg gaagcattcc cagagtggtg gtgagcgaag
    10381 tcataagaat ggggcgaaga cgggtgcggg ccgcctcaag agcggccgca tggggagtta
    10441 acccgcgctc caccaggcga tttaaaaaat cgacaaggat aatgctattc gctacagcga
    10501 tcccgttaag cagaatcacc cctagcattg aattcaagga caaagtgctt ctaaaaataa
    10561 acagcgataa taaaacgcca ataagtccca aaggcactga catgaacact aaaagagaat
    10621 tgatcacatg acccagttgc aaaatcatgg tgactaagat caagcctgtg cttatgccaa
    10681 gggcccacga taactgatgg atggcttcat ccacttcttt ttcagggtta tcaaaggata
    10741 ccgaaacttc tttagccagc gggctggttt ttttccagtc ctccaccaca gcttccgctt
    10801 ttttcaaaat caattttgaa gaacttttct cttccttctt caatcgtccg tgcagcaagt
    10861 attcttcttg ctgatccaag cgataaatgg agggacgagt tttttcaatg ttgatatgag
    10921 ccagcgcctt tagcggaatg agctttccag cgacgctcaa gggaagggcg ctgagttcct
    10981 cggtgctttt gataaatcca gtggaatatt tgagacgaat gcgagtcaat tgattgttca
    11041 tggtcaaata accgatggtt ctgccttcgg tggccacgcg gctgtagtcc gccagatccg
    11101 aaggactcac gtgagaaccg gcttttgcaa tctcgggcca aagttctagg cgcggctgaa
    11161 ctgaaatccc ttcagggttg ttcgtctccg gcaccgccca gagactgtga taatactgtt
    11221 tctcttgaag gccatccaag atctccttgg tcgctaaaag cattttgtca gtgtcttgac
    11281 tgcgcaccac cactaataaa ttctcaggat ctggcagagg caattccgaa ggattccact
    11341 gatccaccca gtactgcgtg gtgggagtcg cggggaattc attttccaag cttttgataa
    11401 tggcggccat gtctttttta tctttaagac ggagcatgat gttgcaatta ctcgggctat
    11461 ttatttgagt gaaagtataa aggacctggc cattaaggaa cttcagcagt tttgcttcta
    11521 tttcgtcgct ctgggtttcc atctggcgga ccagggtatt gccctgagtg ctcattccaa
    11581 agatgaccca atccgtgtcg ggtgtgccaa tgatttcttt cggcaaccgc ggcaagacaa
    11641 attgaatcag caccagtaaa ctcacgacaa gaccgccata aatgctcttt ctccataaag
    11701 aatttttaat gaagagatcc agggtcttgg catagaaatt ctcaaggcgc acaaagtatt
    11761 tttccaaagg agaggccgga tggaagtgag tttcgctctc catcatctgt aagcgaaccg
    11821 tcggaaccaa gatcaaggcc acaatggcgg aaagaccatg ggaaaagacc acggctttcg
    11881 cgagatctcc taaaattgca gaacttaaac ctttggtgaa aatcaaggga gcaaaaacca
    11941 caatagaggc aatggtcgaa gcaatgatcg gcagtttcac ctcgctgaca gcatccatta
    12001 agagattcag cttggcctta taatcgagaa cacccttggc cttttcgaaa tgacggaaga
    12061 tattttccat caccacgacg gagccatcca cattcatacc tgcggacaag gccaagccac
    12121 cgagagagat taagttcaaa ttcatatccg tgagcttcat taaaatgaaa gccaagacga
    12181 tactcatcgg aatttctatc gccgcagtga cgacgttttt aaagctgcca ataaataaga
    12241 agaggacgag cacagccaag ccggcggcca gggccacttc atgaatcaca ttattgacgg
    12301 aggaccgaat gaattcagaa ggatcgacga tgattttgta ttcaatatcc gttggaagaa
    12361 gatgtttctt ggattcaata atctgcagga tatcttccga cattttcttg atattaccgc
    12421 cggattttgg atccgcgaat aaaatcacac tcaaggcgcc actggtgcgg atcacacgat
    12481 ttcgatccaa gggcactgcg tagtcaatgt tcgcgacgtg ggtcaagtga atggtttgtc
    12541 cctgcggcgt ggtgatcagg actttttgca gatccttgat cgtcactacg gcgctcttca
    12601 caattaattg cagtcgctta tcgccgattt cgagatttcc gccttgatag ctctccatgg
    12661 ccgacatcac cgcacgggtg atatccacgg gaagaagctg gtacttggtc aacgcatcag
    12721 gattgatctc gatttgaatt tctttttcag aaggattcca taaagtggca ttggaagcgt
    12781 cagaaacttt tttaagctcc gccgtgaaaa ggggatcgag aattttataa agttcgtcca
    12841 aatcccgttt cggactataa aaacttgcgg cgaaaaaacc gttattttgc gaccaggtgt
    12901 aaacattggc tccacggcgc acttcctcgg gcatgctcga agtgacttgt gcaaccaaag
    12961 tttccacctc tttcaaagca tcattgggcg gcgtttccca cgggaaagaa accgtataag
    13021 tcacattccc tttgccgtag cgggcttcca gtttttctac tttaacgtat ttggtgttga
    13081 gggatcccag cgaagattcg aggctttgac cgtagctatt taagaaatct ttggcggtgg
    13141 tatctccata agggatactg gcttggacga cgggcttgct gctattagga aagagggaaa
    13201 tcggaagact gagtccgctg aaaataccga ccagactcag aactcccagc atgaggtaaa
    13261 cgcgaagagg acttgaatag agaagtctca tattttatct ttttcgactt tgacggcttt
    13321 attgtcttga acatattgac tggagcgttc aatcaaaagt tcgttcacgg aaagaccttt
    13381 tttgatctcg acttggcccg cttgacgaga gcctaactcg accgccttgt attttgcaat
    13441 attatgagtg tctatcaccc gcacataggg cgtagcccct ttataaacaa tagcctgctc
    13501 aggaatttta atcgcagaac ctttgttaag agtgatttgg acttttccaa tggtccccgc
    13561 tggtaatttc aaagatttct ttttaaaaag cagattggct gtggccgtcc cagtggtaga
    13621 gctgactagg gggctaatcc cttcaaactt caactcttct ggtttttcag agagagcggt
    13681 agagaaactc actgaggagt aaggggtgat tttttccctt actttttcga ggtcacttcc
    13741 gggaatttcc acggtgacgc gcaattggct agggtctacg accatggcga tcttttgtcc
    13801 ccgcgccacc tgagtgccga ggctgacatc cacctcgctg acaacaccat tgaccggcgc
    13861 tacgatttta gcggcagcgt attggtaaac tggatctaac tgctgaattt tgaaaagcac
    13921 ttggcccttt ttaacagagg agccgagagt ggccacttcg cgaaccacac cttcaatttc
    13981 ggcgagaatg gcgctgttga cccgggattc tacgcgggct ggataggaaa aggtctcaga
    14041 agactcggtt aaggttattt ttttgatttg aactatgggt acgacctcag gagctccctt
    14101 ggccgctgta tccgttgctg cccaagtgaa ctgccctaat aagcagagga accctactaa
    14161 actccacttc ttcatgaaaa gcccttcgcg taaattatgc ccaattgtcg tttttatcta
    14221 ttattacttt attacttgat taacataaca ctgcgattgc taccatgggc tatggcacta
    14281 aatgtcattt tttcaaactg ccagaggtga agaggggaaa agaggatttt ttatctttaa
    14341 attagacaaa tagtacgcaa gttcgatgac agatggcaat gttcgatcaa aaatttgttt
    14401 agatgttgta aattatcaaa aatattggtg attctttaag gcggtgaggt ttatgaatcg
    14461 aaatttgatt caaaataatg aaaactgcag gaattgcccc atggtgatgg gtatttctca
    14521 gacaattgaa agtgaattca acaattggag tttaaccacc gctgaaaaag aagtttgcag
    14581 agctctttta atgggaaaaa gtctgcgtaa aatttctcag gagcgccaca ccaatgaaat
    14641 gaccgttcgc caacaagcct ttgcggttta tgccaaggct ggcatgtcag gtcgccatga
    14701 gatttcggct tattttttaa aaagattctt tcagattact tcagatttat aatttacaat
    14761 tttactcttt ctaaggattt aacgatgggt cttcgtgtaa gggttatttt tgtgctgtgt
    14821 ctgttaagct ttttactgac cggcactcag ctcctgttca cgctcgagca aattgattca
    14881 gaaaaaactt cggctattgt gacaaagtcc agggcgattc tctctcgact cgaaagcgtt
    14941 cggaattata tcgcggaaca aggaggctta gtctgggctc tcgaaaatgc aaaaaaaaat
    15001 tatcctgatg gaaatctacc aaaagacgtc aaattagcga ttttgaaaca ggtccccatc
    15061 tttgcctcca tgcaggttgg agcccaagag gcgcaaaaag aaatgtatca atttcgagtt
    15121 ttttcagatc agccccgatc taaagaaaat caagcgactg aaaatgaact cgaggttttg
    15181 aagaggttcg aagcggatcc aaacctcaag gaaattcaat caaaaacatc ggaaagcgtg
    15241 atcgtttatc gtcccgtcag gctctcagaa aaacaaggat gcttgaattg tcacggaaat
    15301 cctgaaaata gtccctgggg gaacgggaaa gatgtcttag gtttcaccat ggaaaattgg
    15361 cgagatcaaa agctgcatgg ggtttttgcc attgtttccg atttgaatct tgcggcaacc
    15421 cgtgaggcga aacaaaagca actgctgatg attttgctga cgagtggaat cataacttta
    15481 tctggactgg ctctcgctta ttggattttg agcaaaccat tcaagcaagt atcggtggtc
    15541 gcggaaaatc tgagtgcttc tggaaacgaa gttcatttgg cgagcgcgga aattgccaaa
    15601 gccagcctca atcttaatga gtcggctgtg gaggccgcga attcacttca agaaacttcc
    15661 gtcaccatga aatttatgtc tgagaaggtg agtctcaatt caaagagtgc ccagatggct
    15721 tatcaactat cggcacaatc taaggagtct gcagaaaaag gcttccagga gttttcggtc
    15781 ctagtatctt caattgaaaa catcaaagcc agctcgtcaa aaattgagca aatcattcat
    15841 ctcattgatg agatttcttt tcagaccaat cttctggccc ttaacgcagc tgttgaagcc
    15901 gcccgagccg gagagcaagg caagggattc gctgtcgtgg ccgatgcggt tcggactttg
    15961 gcgcaacgga gcgcaagctc agccagagag atttctgaac tcattaaaga aagcgtggaa
    16021 agaattgatc agggaaatga aattgctctt cagagtagga cttcgctgca aacaattgtt
    16081 gaatccgtgg aaaaggccgc cgccttaaat aaggaaattg ctgatgcgaa cgaagagcaa
    16141 gcgcgggaaa ttattaatct caataccact atgagtgaac tggacgcagt cacgaaaagc
    16201 aatgcaaaga acgcggagca aacctccgcg gcctccgaag agctaagtgc tcaggcgcaa
    16261 cagttgcaaa aattcgtgct ggatctcaat agggtgatcg atggaagaaa agaagaagct
    16321 gacttataaa ctgaatgcaa ttcccgtatt caatccatag gtcacggcaa acagattcat
    16381 attagtggtt cccaaatatc catcaggttg attctcgctg accttggtaa tgcttgtttg
    16441 ataatccatc tgcatagtcc aagaaattga ttttgtgatt ttcacgccgc cgccgatgcc
    16501 tgcagcccag cgaatatcgg tggagccaaa atagtttttg gtattgccat cattgatcgc
    16561 cactcctgaa gaattatagt acattccata atagttgccc atagcggtat aattttgggt
    16621 attcgaaatg ggtccagtgg agtcctgcag aaccgcaacc acggcaccgg ccttcaaaaa
    16681 tatttttgca tctcgtgaaa gttcataatt ataacgaatt aacgtaggca cctcgatata
    16741 gcgattattg tagtcgattt gccaatggct aatacccaat tgatagattt ctctactgcc
    16801 cctttcagca tagagcaggc ctgtctcaaa ttgaaatttt ccgcgtccaa tcaggaaacc
    16861 caggccacct tgataaccag tacgggactc aaacttatag ggacctgatc ctccggtgaa
    16921 gttagagaaa gtaattccac caacaggctc aaaagtaagg aggggtttta taggctcagg
    16981 ctttggagtt ttagtaacca agccagcagc ggtggtggag tccaccaacg tcggttcctg
    17041 agtttgtgcc tgcgccctta tttgcattgt aaaggcaaaa aggagaacca ataagttcat
    17101 gcagtttttc ataaaatttc tcctatactc aataaaaata aaattccagt aactttacca
    17161 gtaacttagc caacacacca attcatccaa gctaaatgcc aaattcatgc cgcgctaaaa
    17221 agtgacttat ttttatttga gagaaagaag gtgtctaagg tattttttat atgactttta
    17281 ttgttgctgt ctaaaagttc gacgacaata tagggtgcca gtttctaagc cgatagcaaa
    17341 tgtgaaacca gggcatagag tccggcgcaa accaagacgc cgccagcgat tttttgtttt
    17401 ctttcattgg atttctgcag ggccggtcga atcatcattg agaccgcact caaagcggga
    17461 agcccgccca gccagaaaag aatcatcgtg aagccgccgg cccaaggact cttggtagcc
    17521 gaggccgcaa gcacataagt ataaagccac atgcaaggta ataatcccgc catgagtccg
    17581 atgacgaaac tactgcggct gaacttgaat attttgattt ttaaataaag ccgctgaaaa
    17641 agttttccag cccagtggtg ggagttctgc caaatatttg ccgataaaag atactgaagc
    17701 cccatcacaa tcaaagtgat tcctaaaaga aaagcgccgg tggcgcgaag ccagcggaat
    17761 tctgatttca ggacaaattc tccgaaaaaa ccagaggctg ttcctaggga aaaataaata
    17821 atcaatcgtc ctaggtgata gtgccacagt tgaccgcgtg agccagtcag ggccgcaaag
    17881 ggtccgcaca ttccagcaca atgccaactg ccgagaaagc tggaggcgat gatggctagg
    17941 ggcaaaagaa gctgttccat tttattgttt tggtccgatc agcgtaaatt ctttttctga
    18001 cttaaaagga ctctccactt cactttgatc ctcaataaga atctgagttt tgagttggcc
    18061 ggtttttgcc acaagctcag gtttaaaacg aataaagaaa tgccactcga tcatttttcc
    18121 aggttctaca ttgagagggt tttgccccac tgtaagttca atcccttgtt ggctccagct
    18181 tgtagggggc cttaaaagat aaatcgcttt tttaaagttt tgattttgaa tctctaaacg
    18241 gaaatgattg agaatcatat tctcgccctt atcatttttt atcaaagtgt agggtgcttc
    18301 ttttgctcgt aaaataaaga cctgggcttt ttcgcgagtg ctaatattat aagtaagccc
    18361 gatgagagaa agcagaatca gagccatgta aaccagagag cggggcttgg taagagtgag
    18421 tttactatga tctaaggtgt cgtaacggat gaggcctgtc ggttttttga ctttgaccat
    18481 gatttcatcg caggcatcag cgcaagccgc gcaggaaatg cactccattt gcagaccttt
    18541 acggatatcg attcccgtcg ggcaaacctg cacgcaacgg ttgcaattga cgcaatcgcc
    18601 ttgtttttca ttcatcgagg gtttcccttt acggggctct ccacgtgctt gatcataaac
    18661 cacagccaag gagttttgat ccaagagaac actttgaaag cgaccatagg ggcacatgat
    18721 gatgcagaac tgttctcgaa accagccgaa atcgaaaaga accaccagag tgataaagct
    18781 gacgatcaga aaggtctccc aattctgtcc ggggctttct tgcagcatct tgatcattgc
    18841 ttcagaaccc acgaagtaag ccgcgaagct atgggcgatc aatgaagaaa cgacgaaaaa
    18901 caaaaaccaa gtgagcagag ttttgaaaat tttagaaaaa cttaatggag cctgtttgag
    18961 tcggcggcgt tgaatatagg ttccttgtat ccatctttca attctacgga aaacactgtc
    19021 aatgaaaacg gtttgagggc aggcccagcc acaccaaact cggccccaaa tagaggttac
    19081 aaaggccagg cccaaagtga gagtcgctaa gataaagaaa accaaaggcg catcatgagc
    19141 ccaaaaagta actccaaaga tcgcgaattt tcgggcgctg atgttgagta aaatggtttg
    19201 ggtgccgtta atgctcgtcc atggcagccc tagaaagatg accagtaaaa taatgtgaat
    19261 ccagttttta taccgatgat aaaatccccg gacttccgca ggaataatat aagttcggtc
    19321 tccaaattca tcaatactag tcagtctttc cggatctaac gccatttatt tccttcacgt
    19381 ctctatttta tttcgatgta atttcgactc cttgtggagc cttaggattt gcaggattgc
    19441 tgcctttctt tgaaagaata aaagcagtca ttccgtaaac ttcctctttg cttaaaagag
    19501 tttcccaagg aggcatgcct ttgtctgcaa cccctttacg aatcacagtc acgatatcca
    19561 tgggagttcc tttgccgtga atccaaaagt tatccgttaa attgggaccg atcaatccac
    19621 ccaactgagg tccatggcaa gaagcgcatt ttgtagagaa ggcggcggcg cccacctcca
    19681 cagctttggg attcttaatg gcagcagtca gcatgtcttc gctttcggtt tccgcgggcg
    19741 catgttgagc ggtggtggtt tcgatgctct tcatggcgat atcaagctct tgggccaagg
    19801 tgagtcctcc gccgaactga tagtggataa aataaataaa ggcaaatatg atggtgatct
    19861 gaaaagtcgc cagccaccat gttggaaggg gattgtcgta ctcctggatg ccatcataat
    19921 catggtctgt cagagggtcg ttgattgaat ctttaatatc actcatggtt agttcccttc
    19981 tttcagaggc aattgttcga ggtctttata gatagcttta ttggctttga gtgaggtcca
    20041 gcaaaggacc cccataaaga aaacaaaaaa tatgatcagt cccaatgatg tcacttgggt
    20101 atcggtgaaa taggaaaagg cttctttggt catggtgttt ttcctttttg tcctaaggat
    20161 tgaagataag caatcatggc gataatttgc ttttgctcaa gctttggaat attgatgcca
    20221 ttggctttaa gatcttccgc tacaaccatt gcttgctttt gggcctcaat atcggcattg
    20281 gccacggtat tgtcgtcata gggaacgccc agcattttca tgactgaaag ttttttgcga
    20341 aggatcagga agtccgtgtc tttctctgtc agccacatat agttaggcat aatggattta
    20401 ggggtcaccg cacgagggtc tagcatatgt tggaagtgcc aaagattcgg atacttctta
    20461 ccaacacggg caaggtcagg accgattcgt tttgagcccc attgaaaagg tctgtcgtac
    20521 atggattctt caagagtcga agcttttcca tagcgcagaa cttcagaagc gatcggacga
    20581 atctgttgag agtggcaaac gtaacagcct tctttaagat aaagatcccg tcccgcgagc
    20641 tccagcggag tgtaaggctc caccatattg ttaggggtaa tatatttatg caaagacaac
    20701 gttgggtaaa tttcaatcac gctaccgaca acaatggcca agaaagccag aactgaaaat
    20761 accgttgcca ttccttctag ttttccatgg cccacggctg catccattct gctgaaaaca
    20821 gaggcccggt ttggaacctg aaccgccact tcttcttgcg ctttcggcgc cgattgaatg
    20881 gttttataaa gattgtaggc cgccatgaag tagccggcaa tgaacaacag tccaccgaaa
    20941 gcacgcaccc agtacaaagg cacgatgcga atcacggttt caatgaaatc tggataaact
    21001 aaacgaccct ctggtgagat ggctctccac ataagtcctt cggtaattcc agctgtcacc
    21061 atggagatgt aatagagaag gataccaaga gtggcgatcc aaaaatggtt ggtcgccaat
    21121 ttttcagaat agagtttggt tttccaaagg cgggggacga gatagtagag catcccaaaa
    21181 atcatcattc cattccagcc caaggctccg gcatgaacgt gaccgacaat ccaatcggtg
    21241 taatgagcca gcgcactgac ggatttgatg gacagaagag gaccttcaaa agtcgacata
    21301 ccgtagaagg tgatggccat aacaaagaac ttgatgattg gatcagttct gagcaaatgc
    21361 caagctccac gcaaagtcaa aagaccattg atcattcccc cccagctagg cgcccagagc
    21421 atcacgctaa aaatcatgcc aagggtttgc gcccactcag gtaaagcagt gtagagcaag
    21481 tgatggggac cagcccaaat gtagatgaaa accaacgacc aaaagtgaat gatagaaagt
    21541 tggtaagagt aaaccggacg attcgcggct ttggggacat agtaatacat caaacccaaa
    21601 aacggtgtgg tcaggaagaa agccaccgca ttatggccat accaccattg caccagagca
    21661 tcttggaccc cggcatagac aggatagctt tgcaggaagc tcacgggaat ttcaatggag
    21721 ttcacgatat gtaacacagc caccgtgata atggtagcga tgtagaacca cagagccaca
    21781 taaaggtgtc gttcacgacg gcgtgaaagt gtgacaaaga agttcaccgc aaaaaccacc
    21841 caaataccgg caatggcgat atcaatcggc cattccaact cggcatactc tttagactga
    21901 gtatatccta aagggagagt gatggctgcg gctaaaataa taagttgcca accccaaaag
    21961 tggatgcggc ctaataggtc tgaaaacagg cgtgtcttca aaagtctctg agtcgagtga
    22021 taaatcccag cgaaaatagc atttccggca aaggcaaaaa tagcggcatt ggtatgcaga
    22081 ggtcgaagtc ggccgaagct gacccactcc atattcatat tcagtggcca ccaggctaac
    22141 tgaattgcgg caatcaagcc gaacaggagg gcggctcctc cccagatcag ggtggcaaga
    22201 atgaatttct tgacgatatc atcatcatag aagatttttt ctgtcagatt atctctgaga
    22261 ttatgagggt tgagttcggg gcttttcacg gcaattcctt tcttgtggat catcttgtaa
    22321 aattctatga gctggtgttt caacatcatc aaattgacct tgcgaggtgg accataaaaa
    22381 ggccaccaca aaaccgaaac caagaagcag ggccatgggg atcatgagga tgagtatatt
    22441 catcgagtcc cccttaatga agaaataata attaaaacag aactgatcgg catcagtatg
    22501 gccgccatca tgggattgat aaatccaccg agggcgaggg ctccgccgag accattataa
    22561 aagagcgaaa tgaccagatt gcgtcgcaca gtgcggcgtg cttccttgga gatttgcata
    22621 agctctaaaa gaggttgcag gccgccacga gtaaagtaga cgtctgcgga catcaagctc
    22681 aggtccacgc tgcccttcac ggcaatgccg acatcggccg ccttgagcgc taaagaatca
    22741 ttggcgccat ccccaatcat acaagtatgt tttgcagctt ggatctcttt taatttattt
    22801 tcaggagaaa gttcggcgtg acagcgagct tctggaatac cgcagaggct cgcaatcttc
    22861 gtgacccggg gacttttatc tcctgaaaga atgtgcaatt gaaattgggc aagttttccc
    22921 aggatttctt ttgattctag gcgcagagga tcttcaaaaa agagatgagc cataaggcgc
    22981 ccctcaattc gatattccac agcgatgtag tcaagaatat tgagttcttg gctgcgggtc
    23041 agggagtagc gggtgttatg aactaaacca ctgacccctt cgccaagaat ttcttgatga
    23101 tcttcaactt ccgggaggtg ttgtaaagaa ctccaggcgt gacggagtgc aaaggcaatg
    23161 gtgtgagagc tttttgtttc aagtgccagg atgattttcc gatcctcttc ggagaggtct
    23221 ccatcaatga gggccagttt caaacggcct tcagtgaggg ttcctgtttt atcaaagaaa
    23281 atattttcta tgtgctgaag tttttcaaaa atatcggagt tgcggatgag gatgccttgc
    23341 ttctgggctt tgcgcaaaga aagaccatag gcgaggggcg tgccgaaagc caatgcgcag
    23401 ggacaggcga tgacgatcaa agccaaggct ctttctaaag cggccgtcgc gtccactgcg
    23461 gaataaaaaa taaaaaacag caaagcgata ctggagaccg tgacgataag gccttgcgcc
    23521 agtttgtccg tgagactcac aaaatgactt ttctgaaccg agtctgaacg caattgctgg
    23581 agaatttttc ccaggcgggt ttcgagtgct attttctcca ctagcaagag aatctttgga
    23641 cttaacagag tggaaccggc aaagagggac atgccctcgc ggaaagactg cggcaatgat
    23701 tccccattaa aaagagacat atccacttcg gcctgcgaac tgaggagaac tccatccgcc
    23761 ggaatttttt ctccttgcag gaccgtgatc tgagcgcccg gggtgagatg tcggtgattt
    23821 tgcagtcctt ctaaaagata gtccccactg agatgttttt gttgaacccg ttttaacaga
    23881 taacggcttg aaaggatcag gaaaataaaa ctggccgtgg aatcatagta aaccggtcct
    23941 ccgacgcgga aaactagatt ataagtggag agaataaaac cagagagcat cgccaccgcg
    24001 atgggaagat cgacattgac ggcccggtat ttcaaaaaat tccaggctcc ttgataaaag
    24061 ggccaggctg aatagaaaag gatgggcagg aaaagagcga aactgatcca attaaaaagc
    24121 tccgccaaag ttccggccag ccctgcatag accggaacta caaacagcat catattgccg
    24181 gcacaggctc cagcgacccc tatacgcatc agacttttgc gattttcttg tttttgtttt
    24241 tctaaaagtt tttcactcga gacaatggcg gtgggggcat agccgagttc ttcgataagg
    24301 gctgctacct gagcccagga ggtgttgggc tttacccgaa catgaacagt ggattctcca
    24361 aagtgcaccc gtgattctaa aatatttgaa tcaaattccg gcagcctttc caaaaggtgg
    24421 acgcaggaag aacagtgaag tccttctgca tgaaaggtga cttcgcaagt attatgagcg
    24481 ctcagttgtt catcgagaaa tttatatttt tccaggctca gggagaacga aggttttttt
    24541 atacctttat cgtgggagtt ttcgagttta tagagagctt cgcatgcggc gcagcaaaac
    24601 gaaggctgag gagggctcag caatgttttg caataaaggc attcagataa aggaagaact
    24661 atatccataa ttaaaggacc cgcttttgcc gttagattag ttctattttg actctataaa
    24721 tatgatggct gtcatgtttc atgtccagcg ctaaaagttt atagattggc tttagacttt
    24781 aacagttctt tgtttaagat tgtgatttgg cggccatttt gtacgagcag ggcttctttt
    24841 tcaaactgcg cgagggtgcg catcactgtt tcgggagtgg tgcctgccca ttcagcaatt
    24901 tctcgtcgag tccaagtctg gctctggaag tggtcttgta aaaaaatgag agcttcggca
    24961 atacgttcgc ccgcctcttt gtccacttga tggatccagc gttcttcagc cagtcgtaaa
    25021 tctttggcca aatggttcaa taggcggaga gccacctcag gatgattctg aaaagttaag
    25081 agtatctccg ccttaggaac aaagcagagc tccaactgat ccacggcaat ggcggtggcg
    25141 tgatagggtt catcagcgaa taaagaacga tagcctaata ctccgccggg gccaacaatc
    25201 cgcaaggtat gggcagagcc atcttcagaa attttctcga gtttaaccaa gccgcttttt
    25261 acaaaaaaca gccccaaagg atcattgtca gcgtagaaga tcacttgatc tcttttgtaa
    25321 gaacaagaga tcttagatcg ttctagatat ttgggaatat gaggactact ggcgcaaata
    25381 atattttgcg gatttgctgt gcacaaagca cagttaggag aatcgtgatc gtcgtccgtt
    25441 ttttttgtag aactcattta aagtatctta tgtgaaaaag aggctaactg caacaaatgt
    25501 ggggataatt agacaacttc gtcaggtttg agggcgcact caaagtgggt tgaatccccg
    25561 ctttcaattt gaatcgtggg gtgatcaatt ttaaagtttt ccttgaggtc tttactgatt
    25621 tttgccagga gttcatccgt ttgaagttct ttaggcataa ctaaatgaac cgtgagggcg
    25681 gtctctgtgg tgctcattgg ccagacatga agatcatgca cttcttgaac tccctggatg
    25741 ccggtaaagt attcgcgcac gcggatcaaa tggattccgg cagggacggc gtccatggcc
    25801 atattcacgg attcctttaa gaggtcccag gtgcccaccg tgataatcag ggcaataaaa
    25861 atactgattg ccggatctac ccacagccat tgagtttgtt ggatcaaaaa gcccgcaacc
    25921 accacaccca ccgagaccag ggcatccgtc gccatatgca aataagctcc gcggatatta
    25981 atgtcttctt tgcgcccaga cataaagaaa taagcggtga ctccattgat aagaataccc
    26041 acacaggcca cgaccatcac tgtctcggtt tggatgggag tgggttccca gaggcgtcga
    26101 agggcttccc aggtgatccc tcccacggcg attaaaagta aaaccgcgtt ggtgagagct
    26161 gccaagatgg aagatctgcg gagtccatag gtaaagcgag cagagggctt tttgcgcgag
    26221 agccagaggg cggcccaggc aaggaccaat ccgaccacat cgctgaagtt atggctcgca
    26281 tccgcgacca gggctaagga tttagacact aaaccataac cagcttcggc gatgacaaag
    26341 ataaggttta agaaaatacc aatggcaaag gctttgtcaa aatttcgggg ggccgcgtga
    26401 ctatgagcat gactttgacc atgggagtga gcatgggagt gactgtgggc atggtgagaa
    26461 ttgggtgaca taataattcc tcgtttccct ataatataag ggaggcctta tattgcaagc
    26521 gctctttcag gctagggaag ctttactcgc tttcaattta ttcataacct agggcctata
    26581 tgaaatcaca cgtagttatc attgggggcg gactgtccgg agtattggtt tccttggagt
    26641 tggcgcggct tccgcaggga cccgaggtga cactcatcga aaaaaatcca gagcacctag
    26701 gccgtggcgt ggcttatgac tctcatttta ctcatcagcc cttgaatgtg atcgccgggg
    26761 ggatgagctt atttccggat aagccgatgg attttgttga gtggctgaag aagaatcatt
    26821 ttaaatataa acatctcgtt gagcaggttt cgtcccagga atttataccc cggaaaattt
    26881 tcggtgatta tgttcttgaa aatttgcaga gagttcagcg gcaagccggg ggaaggttgc
    26941 agatacgtat tggtgaagcc atttctattg ttgattttgg tgagcgcaaa aaagtgattc
    27001 ttcattcggg ccacgagctg ctagcggacc aagtgatcct ggctctggga aatttccccc
    27061 caggagatct ctttcctgag gaggactcgc tgcaaaagaa cccgcattat tatgccaatc
    27121 cctggtctga caaagtttat agtcatatag agggaaatga aaaccttctc ttggttggag
    27181 caggcctgac cgcagttgat atcgtattag gattaatttt aagaaagttc aaaggtaaag
    27241 tgacccttct ttctaggcgg gggcgttggc cactatcaca tgatctctcc catcccgttt
    27301 ttcagttcgc agagccagaa atccagcatc cgcgaaaaat gctgctgtgg ctccgtcagc
    27361 tgatcaggaa aaatccccaa gttccttggc cctcggtgat ggatgggctc cgtcctttca
    27421 cgaaaaaaat atggattaag tggagcattg acgaaaaaaa atattttctc aaaagactca
    27481 agccctattg ggaaatcgcc cggcatcgaa tcccgacgaa atcaatattc cagctcaagg
    27541 aaatgaagaa cttaggccaa ctggaattga agaagggttg tctgctcaac gtccaagctg
    27601 cagagaatgg aattgaagtg gtgtatcgat cccaaaataa agaatgccgt caaatttttc
    27661 aaaaagtcat taattgtacg ggaccagaat taaattatcg taaagttcac tgccccctga
    27721 tccatgatct tctgaagcga ggaaaaataa aaacagacga gttggggttg ggtcttgaat
    27781 gcacccccga aggccaaatc ctaaatgttc agggcaaaat agagcagggc ttttggtgca
    27841 taggtccgat gcgcaaggcg gtcttatggg agaccaccgc cttgtgcgaa ttgcgcgaac
    27901 aggcttcaga gctggtctcc ttgatttata aaaaagggct cagtccttgt tcttctttgg
    27961 aacaatctga gtgatcgcga tcgttgcatt tgcaacttct tcaagattaa accacccatt
    28021 gaattgaatt gtggaagcct cctttcggga atccataagg tgcacttcgc aaacttgagt
    28081 ttctggattt cttctcaggg cgttggctac aatggcatga cccgcaacgc aatctccacc
    28141 gggaatcatc atattttcaa tcaggctgaa tttttctggg cagatactga gggccagact
    28201 tcttccagtt ttaaaacgct ccacgatgac tttcatttga gtcgagactg gtaaactgaa
    28261 gttctggatt tcttgagaag tgtaatagtc tagaattcgt ggattcaaat cgggaaagat
    28321 gcgagtgaaa acagtgccga ttccagggaa tttcacccgg tgttgtttgc aagcatttga
    28381 aataagagtt cggtccagga gctcaacagc gtctttggat ttttttagag cggtcgtcag
    28441 ttgtgcatca gtaaagtcgt tgtgggagaa ggtctgacga aattccttca gcaaatttgt
    28501 ttgaagcgat tggacctgag cagaggaatg tttcgcctga gatttatgat tgagtgcata
    28561 tttatattca tcgaaaagct taaaaagttt tgactgttga aaacgcttgc tcatggtcaa
    28621 gcggcccaaa aaggatggtt tcattctaaa gtcgacaaaa ggggccaggt ggttggggca
    28681 cacaccttga atttgcgcgc aactcagggc tcgatctaca acattctctt catgattatg
    28741 accgactctg gggtcacaag aggttaaatc gactgcggag aagttcactc cgcagtattt
    28801 cggatccatt aaacaggcct cctcttcgat cagtgaagat cccgccattg cccagcaaat
    28861 ttgatgaaaa ccttgatttt ttaaaggccc aaaacgctcg cgaaagtcca cggatggaca
    28921 agggtctgct tcggcggcaa aggactgcga ggaagtgtta aaaagaaaaa atcctatgag
    28981 gaggaagagt ccaatgaatc taaacatatc gataccctta atggttccct tctgatgcag
    29041 ttgaaaattt atttaaaaag gttttcactt cattgttttt ctgagccaat tcactgtaat
    29101 ttttaagatc agggcatact ttttctttaa gcctgtcatc gattttataa tggctaaaag
    29161 gcgccgagtt tttaagttgg tcaaatttaa caatttgttt ccagatggct ctataaggcg
    29221 cttcataaga attatgggcg ttttctatcc agtaactcag agctgtcgcg ggatcggcat
    29281 tacagaattg ttggagcaag aaagccgccg cgcgccccga ggcatgccag ccgttataac
    29341 aatgaacata gatcggtctc tgcttgtttt ccgcattcat aatttgttca tgtgtcagct
    29401 tgatgagggt ttccaaatct tctgtctgcg actttgtctg aaaggcgcgc tcgggttgca
    29461 agcgaatgta atgcaaagta ttgttttcat tggcctgggt tttacagttc accattgaag
    29521 ggcctttaaa gtttttcgag taaagataaa cggcattgga aaatccccga atgcacagat
    29581 tctttaaagc aatctccggc aggggatttt cgttttcttt gaagccgaat tcgtggttat
    29641 aaacgttgtt agctccgccg cgaaacacaa gaccatttaa gaccgactta agattgcgga
    29701 ctccataaaa cttatgatct ttattgctac aagccgattt ctttatgatt tttccagcat
    29761 aagtttgctg agtggcgcgg gtccggttga actctctagc ggcttcagca ctctctgctc
    29821 cctgaagctt ggtgcttata aactctaatt tggcctgact tctcagctca ctttcgagag
    29881 gggcttcggt aatattgaac tccgcgaaat cacgctcatt gagagcaccg cagtcgttga
    29941 cgatagaaat gtaagtggga tcccccgtta aaccttgagt cttagggggt gccacttcct
    30001 ggggattggg agttgaatta ggagttggag ttggattcaa ggccttatac ataaacggaa
    30061 ataaaaataa gaggccgata aaggtcatgg tttttttcat tttgggtgct ccactctaca
    30121 gtcatttata aacttgaacc agggtgacct tgtcaaaaaa aacatcctat ttagttcaaa
    30181 cagacctctt tgtttttata tagagcaaaa tcatagccaa aaaactttcg ctacaggctc
    30241 agggaaatcg ttaatgatcc ctcctaatcg aataagctgg agtttaaatt tattttgata
    30301 gggattttta tttgagaaca agaaaccagc ttttgccgac aaaaatcgcc tctagtgtca
    30361 attgtgtcga cagggggatc gagatcccct gcaagataat catcactacg agcttcttct
    30421 tttccgaaga tttcctctct gaggaataaa aaatagcaaa actcggtggt tatcttgcca
    30481 ggtcatcaga cccaccacct gggaatacag ttatatggcg aggaatgcag cggttaacag
    30541 aaattcagat aggggtcgag atcggaatga aacttgtggg taatggaaag gaggttcgac
    30601 gatgtacgac tatagatgga gcttcattta tcgtaagtga atttagaaaa ccaaaaaagc
    30661 tggaggaatg gcaattagga attttagaac tgccagtgaa gtcttataag gctgatgatg
    30721 cgccaatatt agtgctccaa aataatggca cagcaagaag tttgtctagc acatcacatt
    30781 tgatcggatc agtaactgac atagttgaga agatgtactt tcttagaatt gattcgaaca
    30841 taaatgagac tcaacttata aaaaaactgg aaaacaaaca ttatctcatg gctagtgaac
    30901 cacaaaatta aacataaaca gaaatataat ccgattgcag cggccgtaaa tttcctctgt
    30961 ctggtgcggt cgaacgctca agctactttc cttgtcagct tgaacaaggt cccgcacttg
    31021 gcggcgagag taagaagata atccaaggtt actttctcag ggctgctgaa gatgctgctc
    31081 cagcgggact tggggatatt caggaactca gcgaactcag tgttattgag gcctcgtcgc
    31141 tcgcgttcct tatcgagctg accgataacg gtgcccttca tcgtgacgat ctctgcttcg
    31201 gcctttgtga agccaaggct gcgagctaga tcttctgtgg acttgaagct ggtcgtagtt
    31261 ttcttattag ccatggaact cctccttcat gcgtttaagc ctttggatag cgatttcgat
    31321 ctcctgcgga ggtgtcttct gagtcttctt gcggaacagg tgaagaataa acacatgatc
    31381 cttctgctga acgtaataga aaactcttac gatccctcgg gagtctcgga ctctcagctc
    31441 cttgagacct gggtagcccg acatctgccg cgataccggc atggtcagac tcgctccttc
    31501 ctcaagagcc ctaaacagga tcagcaactt ttcctggatc tctttcgttt cgccttggat
    31561 gtccttgagg acctgtttgg ataagtgcaa tcccatcaat ttttgatgtt ataaaatatt
    31621 ataactaaag tcaaggtttt agttatggca ataaagcttt ttaaattttg gaactagcgg
    31681 ccactctatc ggcggccagg atcaacttgg aggctggttt tccaaaactt cagcgcccaa
    31741 aacggccggt ttgcggccaa actctttcca atataaaaaa atatgtttat gataataaca
    31801 ggtgaagcaa attgggccca tgcaaagtcc ttgaaataca attggaattc aatagagacg
    31861 agatttcgca gaatctcgca agtagccgtg caagatatta tgatccggag acggggcggt
    31921 ggactactaa agaccctctg ctgtttggcg gtggagat
//
LOCUS       DATBTE010000320         3932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2297268, whole genome shotgun sequence.
ACCESSION   DATBTE010000320 DATBTE010000000
VERSION     DATBTE010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2297268"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VIG33_12010"
     CDS             <1..279
                     /locus_tag="VIG33_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HEY8271605.1"
                     /translation="TMHIHPLSVISGTYYVQTPKDCGSIKFEDPRMVGFMASPPRKEN
                     AQERNKRFVELKPQAGNVVLFESWMRHEVPQNKSDIERISISFNYDWI"
     gene            284..1024
                     /locus_tag="VIG33_12015"
     CDS             284..1024
                     /locus_tag="VIG33_12015"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8271606.1"
                     /translation="MATVLELQNIQKKFKDRTILESVNLNLQDGEFLSLLGPSGCGKS
                     TLLRLMAGLENPTSGTLSWHTAEKKTAFVFQEAQLLSWRTVLENVRLPLEIQAKLSQK
                     EQDEKSHFALQKVNLTSFESFFPHELSGGMKMRVSIARALSASPKILFMDEPFSALDE
                     ITRFGMQKQLRTLCEQDKLSVVFVTHSSYEAAFLADRVLLMSAQGGQFLLNEKIIYSQ
                     PREDSLRLSPDYHSHIAKISAKMSESFL"
     gene            1021..1788
                     /locus_tag="VIG33_12020"
     CDS             1021..1788
                     /locus_tag="VIG33_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8271607.1"
                     /translation="MNSLKKINLPALISLLVGILLYEAAVYFSWIPEFLFPAPSTILK
                     TLAENKLDFVKAFLETLQGSLAGLGLSIVFGIALAILFSLSNFLQKAILPFAVFFQTI
                     PIIAIAPLLVIYFGFGMPTVVVAAFIVSIFPIIANTLLGLESVPKPELELFRLYRASP
                     LQCLFKLKLPTAYPSIYAGLKVSIGLAIIGAIAGEFVAGGGLGAMIDSARTQQRIDIV
                     FGALGLLALMGLTCLTFLRLSHKLVQKMRPYGLNLKD"
     gene            1792..2736
                     /locus_tag="VIG33_12025"
     CDS             1792..2736
                     /locus_tag="VIG33_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8271608.1"
                     /translation="MKNNIKIMILSLMLVSVGFAETPKKIKLALNWKAEPQFGGFYAA
                     QLNGEFKKRDLEVEISEGGSGTPTIQMLASGKTDFAIASAEEIILAQDRGSKILGLFA
                     TYQTNPQAIMTHQESHFQSIKETLEAEGVLSMQSGLSYAQFLLKKYPQPKVKIVPYLG
                     GITNFAKNPKYSQQCFFTSEPLIAEKAGLKVKVFLISEEGFNPYTTVVATSEETLKKD
                     PKAAKAFIEAVRSGWQEYLKNPEQANKLMAGINKSMQAETFTKSAALQKSLIETPETQ
                     QRGLGFMSEERWKKLISQLKELKIIKKDLNVKGLYQNL"
     gene            2736..3284
                     /locus_tag="VIG33_12030"
     CDS             2736..3284
                     /locus_tag="VIG33_12030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8271609.1"
                     /translation="MEIHEFGLNDILAVKSFSDRVIGKGYFSEKELEDSFYRSQAKNV
                     MCSFVLTDGKEIFGLRLAYPPGLWIQGKGKKLSSHLWKIPQEEVAYFQTLFLSPEMQG
                     QGWGPKLSEISIRALKKLGAKAIVTHAWKESPNHSSIRYLTKQGFKFIATYPEYWIDV
                     DYTCILDGRPCRCTAEEMILYL"
     gene            3293..>3932
                     /locus_tag="VIG33_12035"
     CDS             3293..>3932
                     /locus_tag="VIG33_12035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8271610.1"
                     /translation="MSISCWLDHSTKSLKKNYDFVIVGGGIAGLSTAYWLQKESPKYK
                     IAILEKNRIGFGASGRNAGFVTCGSTAHFMKLYERFGISKAVEIWKFSEQNRKLLQEH
                     IIEDQGSQIEYSQTGSCTIASTDEQWLQFKKIAEIMKVQQIDVQEIDSTLVTKDYGVS
                     EVVGGIEYKGDGVIHPVKLLTLLKSKLSVDIYENTEVFAIEGQQRSLQEVKTD"
BASE COUNT         1146 a          849 c          850 g         1087 t
ORIGIN      
        1 accatgcaca tccatccctt atctgtcatt agcggaacct attatgtaca gacccccaaa
       61 gactgcggtt cgattaaatt tgaggacccg cggatggtag ggtttatggc ctctccacca
      121 cgtaaagaaa atgcccagga aagaaataag cgttttgtgg agttaaaacc gcaagctggc
      181 aatgtggtcc tatttgaaag ctggatgaga cacgaagtcc cgcaaaataa atcggatatt
      241 gagcgcatta gcatcagctt caactacgat tggatttaaa taaatggcga cggtcttgga
      301 gcttcaaaat atccaaaaaa aattcaaaga ccgcaccatt ttagaatccg tgaatctcaa
      361 tcttcaagac ggagaatttc taagtcttct gggaccttcg ggttgtggta aatccacttt
      421 attaaggctg atggcgggcc ttgagaaccc caccagcggc actctttctt ggcatacagc
      481 tgaaaaaaaa accgcctttg tctttcaaga agctcaattg ctctcttgga ggacggtcct
      541 ggagaacgtg cgacttcccc tggaaattca agcgaaactc tctcaaaaag aacaggacga
      601 aaagtcccat tttgccctgc aaaaagtgaa cttaacttcc tttgaaagtt tcttccccca
      661 cgaactctcg ggaggaatga aaatgcgggt ttccattgcc cgcgccctca gtgcttcccc
      721 caaaatcctt tttatggatg agccgttttc agcactggat gagatcactc gctttggaat
      781 gcaaaagcaa cttcgcaccc tctgtgagca agacaagctc agcgtggttt ttgtcacgca
      841 ttcaagttat gaagccgctt tcttggcgga tcgagtgctt ttaatgagcg cacagggtgg
      901 acagttccta ttaaatgaaa aaataatcta ttctcaacct cgagaagact ctttaaggct
      961 gagtcctgac tatcattctc atattgccaa gatcagcgcc aaaatgtcgg agtcttttct
     1021 atgaattctc taaagaaaat aaatctcccg gctttgatca gtttgttggt gggaatcctt
     1081 ctctatgaag ccgcggttta cttttcatgg attccggaat ttctctttcc cgccccgagt
     1141 accatcttaa agaccttggc agagaataaa ctcgattttg tgaaagcttt tttagaaacc
     1201 cttcagggtt ccctggcggg gcttgggctc agtattgttt ttggaatagc gctggcgatt
     1261 ttattttctc tttcaaattt tctgcaaaaa gctattttgc ccttcgctgt tttttttcaa
     1321 accattccta ttattgccat tgctccactt ttggttattt attttggttt tggtatgccc
     1381 actgtagtgg tcgcagcctt tatcgtttct atctttccca ttatagccaa tactcttttg
     1441 ggcttagaaa gtgtgccaaa accggaactt gaacttttcc gtctctatag agcttcgccc
     1501 cttcaatgtt tgtttaaact gaagcttccg acggcctatc cttcaatcta tgcgggatta
     1561 aaagtttcca ttggcctggc cattattggc gctattgccg gagaatttgt tgcaggcggt
     1621 ggcctgggag ctatgattga ttccgcccgt actcagcaaa gaatagatat cgtattcggc
     1681 gctttgggac ttttggcttt gatgggcctg acatgcctta cttttttaag acttagccac
     1741 aagcttgtac aaaaaatgcg tccctatgga ttaaacttaa aggattaatt tatgaagaat
     1801 aacataaaaa ttatgatact gagcctgatg ctagtctctg tcggctttgc ggaaacacca
     1861 aaaaaaataa aactggctct caattggaag gcagaacctc aatttggcgg attctatgcg
     1921 gctcagctga acggggagtt taagaaacga gacctggaag ttgagatctc cgagggaggt
     1981 tccggcaccc ctaccataca gatgttagca agcggtaaaa ccgattttgc catcgccagc
     2041 gcggaagaaa tcatcctagc tcaagatcga ggatctaaaa tactcggcct cttcgcgaca
     2101 taccaaacaa atccccaggc cattatgact catcaagaaa gtcattttca atcgattaag
     2161 gaaaccttgg aagcggaggg agtgctgtca atgcaaagcg gtctttctta tgcacagttc
     2221 ttgctcaaga aatatcctca accaaaagtt aaaatagttc cctacttagg gggaattact
     2281 aatttcgcta aaaatcccaa atactcccaa cagtgctttt ttacctctga gccgttgatt
     2341 gctgaaaaag cggggcttaa agtaaaagta ttcctcattt ccgaagaggg cttcaatccc
     2401 tatacaacgg tggtggcgac aagtgaagaa acgctcaaga aagatcccaa agccgctaaa
     2461 gcctttattg aagctgttcg cagtggttgg caggagtatt taaaaaaccc agaacaggca
     2521 aacaagttga tggctggaat caataagtcc atgcaggctg agaccttcac caaaagcgcg
     2581 gctctgcaaa aaagcctgat tgagactcca gagactcaac aaagaggact tggctttatg
     2641 tctgaggagc gttggaaaaa attaatttct caattgaaag aactcaaaat catcaaaaaa
     2701 gatctcaacg ttaagggcct ctatcaaaat ctataatgga aatccacgaa ttcggtttga
     2761 atgatatttt agcggttaaa tctttctctg atcgggtgat cggcaagggt tacttttcag
     2821 aaaaagaatt ggaagacagc ttttatagat ctcaagcaaa aaacgtgatg tgttcctttg
     2881 tccttactga tggaaaagaa atctttggac ttcgcttggc ctatcctcca ggtctctgga
     2941 ttcagggcaa gggcaaaaaa ttaagtagcc atttgtggaa aatccctcaa gaagaagtgg
     3001 cttattttca aactttgttt ttatccccgg agatgcaagg acaaggctgg ggaccaaaac
     3061 tctctgagat ctccattcgg gccttgaaaa aactcggcgc gaaagcgatt gtgacccacg
     3121 cctggaaaga gtcgccaaat cattcttcaa ttcgatatct tacaaaacag ggttttaaat
     3181 tcatcgcaac ctacccggaa tattggatcg acgttgatta cacctgtatt ttggatggcc
     3241 gcccttgccg ctgtaccgct gaggaaatga tcctctatct ttaggagact ttatgagtat
     3301 ttcttgttgg cttgatcatt ctacaaaatc actgaagaaa aattatgatt ttgtaattgt
     3361 cggcggcgga atcgccggac tttcgacggc ctattggctg caaaaagaaa gtccaaagta
     3421 caaaatcgcg attctagaaa aaaatcgcat tggttttggc gcttcaggcc gcaatgcagg
     3481 ctttgtgact tgtggttcta cagctcattt tatgaaactc tacgaaagat ttgggatttc
     3541 aaaagcggtg gagatttgga agttttccga gcaaaaccga aaactcctgc aagaacatat
     3601 tattgaagac caaggatctc aaattgaata tagccaaacg ggatcctgca ccatcgcttc
     3661 caccgatgag caatggctgc aatttaaaaa aattgcggag atcatgaaag ttcaacaaat
     3721 cgatgttcag gaaatcgact caacccttgt cacaaaggac tatggagtca gcgaagttgt
     3781 ggggggaatt gaatataaag gcgacggagt catccatccc gtgaaattat taacgctttt
     3841 aaaatcaaag ctcagcgtgg atatctatga gaatacagaa gtcttcgcta ttgaaggtca
     3901 acaacggagt ttgcaggaag tgaaaacaga tc
//
LOCUS       DATBTE010000321        10471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_316431, whole genome shotgun sequence.
ACCESSION   DATBTE010000321 DATBTE010000000
VERSION     DATBTE010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 10471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10471
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316431"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VIG33_12040"
     CDS             complement(<1..341)
                     /locus_tag="VIG33_12040"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8271611.1"
                     /translation="MAQKKIFLIFYILGSFSLGISQSLQAADTLNFATTKVQTLTHTT
                     TTLKEKFTGQFLLLDFWASWCAPCKESLPFYQELQKRYAAEGLQVLALSVDSDVKDAS
                     SFIRKNNFTLN"
     gene            436..609
                     /locus_tag="VIG33_12045"
     CDS             436..609
                     /locus_tag="VIG33_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271612.1"
                     /translation="MKISLVCLFFAFGLGLSACSHFGFGKKCCDKSSCSMEHGEHAKD
                     AKGCCGGECEMKK"
     gene            complement(652..2016)
                     /locus_tag="VIG33_12050"
     CDS             complement(652..2016)
                     /locus_tag="VIG33_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271613.1"
                     /translation="MKPKSLTLFFLLGLLLGCSKEISVTSNQVKGSESPLYSLNGKEH
                     LVEFQALYRSSYTQTKDIDIETLTSEIKRTAQFLFGPLTYRSLGGIQKGEKIQVLADQ
                     AYVQNERVIIPYYYQATWLLRAEAIDENNFQLPLPYSIPDLRTAQWKRCTDSGEGHNT
                     WDFFWYYWEPGRYGCDHRLGEQYQMISVKVGAETPQTQLSFPEYKRLIHDENGEPTLA
                     MTFAFGYIEDTFPPNPFHDSDYGMTQFQNFYARVKSQLSALGYTEQPIYQKDVSSGAS
                     VIGSQFTGMKENVRVRVSVVAAAGVDQMELFAHSYAKKHEGFFAWFGHSRVGSGFDAD
                     RFAAKLRYNPQEFSLTADYQLVYWAGCNSYSYYTLPFFELKAKMNPESDPSGTRNLDL
                     ISNVLPSLFSFNDYNAQVLFQALLNWNTPTSYQKIVSQIEKYANMSGTKVIVNVLGDE
                     DNPK"
     gene            complement(2127..2672)
                     /locus_tag="VIG33_12055"
     CDS             complement(2127..2672)
                     /locus_tag="VIG33_12055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY8271614.1"
                     /translation="MKIKRICVYCGASPGNNPNFAVRARELGHALATHNIELVYGGGR
                     VGLMGIVADAVLEKGGRVIGVIPQKLMDLEQGHTGIQDLRIVNDMHERKAGMATLADA
                     FIALPGGFGTLEELFEVLTWSQIGYHQKPVAILDVDNYYQHLGKFIKHAMTTDLLKPH
                     YGQLFQVTENVEEALELCLSK"
     regulatory      2813..2911
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            2904..3389
                     /locus_tag="VIG33_12060"
     CDS             2904..3389
                     /locus_tag="VIG33_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271615.1"
                     /translation="MFKLYVAIVASVFSLNAFAFSPSGNFPKGPDAVLTPGEVCDHPD
                     AHRYPEGIAYCNRNVSSDLKRAIFVNYDQLGFRTTQMQRMDFKIDHYIPLCMGGGNDI
                     KNLWPQHKSIYAVTDPLEPLLCDKMAAGVLTQKKAIEYITYGKNHLDEIPHIISEVNN
                     L"
     gene            3537..4820
                     /locus_tag="VIG33_12065"
     CDS             3537..4820
                     /locus_tag="VIG33_12065"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY8271616.1"
                     /translation="MAVKTLRSVYVAGAARVPFVKSGTQYRKVTTHELMLASMNHLVA
                     QYRLQEKVLGDVALGAVMNRSTEWGIPREVVLSTQLHPYTPAYFIQRACGTSLEATSQ
                     IALKIASGQMDTGIAGGFDTNSDVAILYPRSFSRKILNLRDSKSVGAKLANFLKFSPK
                     DFRLDLPANKEDRTGLSMGEHTELMVKEWKISREDQDKLTLLSHLNAAKAYDEGFFND
                     LVFEFQGFTRDGIVRKDTSLEKLAKLKPAFDRSPQGTLTAGNSSPLSDGSSTVLLCSD
                     QGLKELGFEPWARFVDAQVAGVDFVHGEGLLMAPAKAVPVMLARNGLTLQDFDYYEIH
                     EAFAGQVLCTLKAWESDEYCRKHLGLSKALGSIDRSKLNIKGSSVAIGHPFAATGTRI
                     VGSLSKMLKGTQGKRGLISICTAGGMGVVAILESV"
     gene            complement(4817..5116)
                     /locus_tag="VIG33_12070"
     CDS             complement(4817..5116)
                     /locus_tag="VIG33_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271617.1"
                     /translation="MKYVFALSTLLFATHSFAVLPPYWDSVRQIEAVIHSTELSGKIH
                     GPITNIKNISNLTYEVSTQSCRATVVLDIKAPTQPGAASYSVKLVSEAICLSLYS"
     gene            complement(5295..5552)
                     /locus_tag="VIG33_12075"
     CDS             complement(5295..5552)
                     /locus_tag="VIG33_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271618.1"
                     /translation="MENSWQEFLKALHSDNETEYGDFKREIDAQLHDLVSSSPPLTEQ
                     QARALVKIRDDYVWTDHTDNEIENIKRDISKRILEISQFSV"
     gene            5680..6243
                     /locus_tag="VIG33_12080"
     CDS             5680..6243
                     /locus_tag="VIG33_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEY8271619.1"
                     /translation="MIESISSSFLLVAASEMGDKTQLLAFSLAAQYKKPLPIMAGIFV
                     ATVLNHALASWGGTWVATRVSPFLMANILGGIFVGFALWTLKPDEADEVDEGSKFGPF
                     LTTVFLFFMAEMGDKTQLATVALGAQFKSAWEVTLGTTLGMLVTDGLAVVVGDQLSHK
                     INMKYMRWFAASLFFGFGILCFWKAYV"
     gene            complement(6306..6623)
                     /locus_tag="VIG33_12085"
     CDS             complement(6306..6623)
                     /locus_tag="VIG33_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271620.1"
                     /translation="MKFAKFILAAALTLPFFAKAQESLPPDLSAPPAPPSMNEPGLEP
                     MPSDGAIIQHTAPALELGETKKVTKKKAKKKSKKEKKSNSKGKKNSKKAKKGKNKKHH
                     PTY"
     gene            6750..7118
                     /locus_tag="VIG33_12090"
     CDS             6750..7118
                     /locus_tag="VIG33_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEY8271621.1"
                     /translation="MSSLKVYEYSGCSTCKKALKFLDARKLKYEVLAIVERPPTKVEL
                     ERMLALVKEEGGDLKKLFNTSGVLYKEMKISEKFSRMSESEALDLLAENGKLVKRPFV
                     LGADFGLLGFKEEQWKKRLR"
     gene            7218..9614
                     /gene="lon"
                     /locus_tag="VIG33_12095"
     CDS             7218..9614
                     /gene="lon"
                     /locus_tag="VIG33_12095"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY8271622.1"
                     /translation="MSIPTGFLPVLPLKNAVIYPGVGQALRVGREKSILALNKAHENQ
                     SWILALAQKSMDQTQALSVEDICTVGTLCKVESIRGSGEMGFQAILRGHQRMKVTNLV
                     LKDQYFQALAEPLEDLIDVDEHTRAVLMESLKNQSRQILRFVPTNTEQLDEMIEKIDD
                     LGLLSYFCAAHIDISMREKQELLEKTSLKDRVMALLVHMQDFKENLQVQAEIRSKVSS
                     KLGQTQREHILREQLKTIREELGEEGQSSTNGDKYTQRIEEAGMSEEAKKVAQQEVQR
                     LKNISPQSPEYQIISNYLDLLTTLPWSKASHEGEIDLEKARMILDEDHYGLNKIKKRI
                     LQYLAVMKLKNNNKGNILLFVGPPGVGKTSLGQSIAKALGRKYVRISLGGVRDEAEIR
                     GHRRTYVGALPGRIINSMKKAGENNPIFVLDEVDKLGRGFQGDPGAALLEVLDPEQNN
                     HFMDHYLEVGYDLSNVFFIATANSLEGIPGPLMDRLEVIEVSGYTTEEKFYIAKNHLL
                     AKQLNEHGILPEKLEVTDAALHHLISHYTREAGVRELQRKIAALCRAAAEKVLSSTET
                     VLIDTKDLEEILGPERYTAEIAENAASAGVVTGLAWTPVGGDILFIESTRMPGTGHLL
                     ITGQLGEVMKESAQIALSLLRSRLPLINPLVDFSKQDIHIHVPAGAIPKDGPSAGVTL
                     LTSLASLFSQRKVDPKLAMTGEITLRGAVMPVGGVKEKVLAAHRAGIEKILLSKKNEK
                     DLIEVPLEIRNSMKFIFVENVNEVLKASLELDVDLMGSIYYQNTALMQPPQQGGVGSN
                     "
     gene            9601..10107
                     /locus_tag="VIG33_12100"
     CDS             9601..10107
                     /locus_tag="VIG33_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271623.1"
                     /translation="MVVIKENGLYLVLIKSVRGIRTDDNLRNNLCPDPHGSANSAAWE
                     LAKAVALEAVLNTENFLDETKNLASALFYTSKITPYWEDDPDFVKYGPVNIKLNFPDL
                     DDKSLKVYNQNVHNPNCVKLYRNKKYLESINKIKNAPHKYYSGNIMKLWKVPAVLSSL
                     QMDRADLF"
     gene            complement(10099..>10471)
                     /locus_tag="VIG33_12105"
     CDS             complement(10099..>10471)
                     /locus_tag="VIG33_12105"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEY8271624.1"
                     /translation="ALKSVSEAAPAMMQLHFLRDVEGEKVQTSFKEALEVNKVDLKKP
                     ELQKFLEAVRIGGEAKKGKSLSILGSKKSDGSEVITYENSNSKIIAISGGPGFIQDIF
                     SIWLGQPSDDGIEKLKADILK"
BASE COUNT         2883 a         2120 c         2494 g         2974 t
ORIGIN      
        1 aaattcaggg taaaattatt tttgcggata aagctgctag cgtctttcac atcagagtcc
       61 acgctgaggg caagaacttg taagccttca gcggcatagc gcttttgcag ctcttgataa
      121 aagggcagcg actctttaca aggagcacac cagctagccc aaaaatcaag caaaagaaat
      181 tggcccgtga acttttcttt taaggtggtg gtcgtatggg tcaaagtctg tactttggtc
      241 gtggcgaagt tcaatgtgtc ggcggcttgc aagctttggg atattcctaa actaaagctt
      301 ccaagtatat aaaaaattaa gaagattttt ttttgagcca taggcataat ttatgtcaca
      361 aatttataat ttccactcta ttatttcata aacacgactc gaaggcgaat cttcgggata
      421 gagaaagaga gttttatgaa aatttcactc gtatgtttat tttttgcatt cggacttggg
      481 ctatccgctt gcagccattt cggttttggt aaaaagtgct gtgataaatc ctcttgcagt
      541 atggaacatg gcgaacacgc caaagatgca aaaggatgtt gcggcggcga atgtgagatg
      601 aaaaaataaa ggtgaaaaat aaaagagagg cccccctttt aggggggcct tttatttcgg
      661 attatcttca tcccccaata cattcactat gactttagtt cccgacatat ttgcatattt
      721 ttcgatttga ctaacgatct tttgatagct ggtaggagtg ttccaattta aaagcgcttg
      781 gaaaagaacc tgggcattgt aatcattgaa ggaaaataaa gaaggcagaa cattcgagat
      841 taaatcaagg ttgcgcgtgc cagaaggatc agactcggga ttcatttttg ctttcaattc
      901 aaagaagggg agagtgtaat aggagtagct attgcaaccg gcccagtaaa ccaactgata
      961 gtctgcagtt aaagaaaact cttggggatt gtatctcaac ttggcggcga agcgatccgc
     1021 atcgaatccg gatccgacac gggaatggcc gaaccaagca aaaaaacctt cgtgtttttt
     1081 tgcgtagcta tgggcaaaaa gctccatttg atccactcca gcggctgcca cgacagagac
     1141 tcgaactctt acattctcct tcatgccagt aaattgagag ccaatgacgg aggcgccaga
     1201 gctcacatct ttttgataaa tcggttgctc ggtatacccc agagctgaaa gctgactttt
     1261 cactcgcgca tagaagtttt gaaattgagt cattccataa tctgagtcat gaaatgggtt
     1321 gggtggaaaa gtatcttcga tataaccgaa agcgaaggtc atcgcgaggg tgggctctcc
     1381 attttcatca tgaatcaatc gtttgtattc ggggaaagaa agttgggttt gcggagtttc
     1441 tgcacccact ttcacggaaa tcatttggta ttgctcaccg aggcgatgat cgcaaccgta
     1501 gcgccctggt tcccagtagt accagaagaa atcccaggta ttgtggcctt cccctgagtc
     1561 ggtacatctt ttccactggg cggttcggag atctggaatt gaatagggta agggtaattg
     1621 aaaattattt tcatctattg cctcggcgcg gaggagccaa gtggcctggt agtaataggg
     1681 aataatcacg cgctcgtttt gtacataagc ctgatccgcc agaacttgaa ttttttctcc
     1741 tttttgaatc cctccgagac tccgataggt caagggtcca aagagaaatt gcgccgtcct
     1801 cttgatttcc gaagttaatg tttcaatgtc gatatcctta gtttgtgtat aggaagatcg
     1861 atagagcgct tgaaactcaa ctaaatgttc tttgccgttt aaagaataaa ggggcgattc
     1921 agatcccttt acttgatttg atgttaccga aatctctttt gaacatccta ggaggagccc
     1981 tagcaagaaa aaaagagtga gtgattttgg cttcatgttg cccccgattc atttctggaa
     2041 gttctatagc ataagcacag gttatgccaa gcttcagaag aaattttgcg gaaatgatct
     2101 acaatcatag gcataaacaa ttctaattat ttggaaagac agagttccag cgcctcttct
     2161 acgttttctg tgacttgaaa aagttggcca tagtgcggtt tgagtaagtc tgtcgtcatt
     2221 gcatgcttga tgaattttcc caagtgttga tagtagttat ccacgtccag gatcgccact
     2281 ggtttttgat gatagccaat ttgcgaccag gtaaggacct caaagagttc ttccaaggtt
     2341 ccaaaaccac ctggcaaagc gataaaggca tccgccagag tggccatacc ggcttttctt
     2401 tcatgcatat cgttgacaat acgcagatct tgaatgccgg tatgcccttg ttctagatcc
     2461 atgagttttt gcggaataac tccaataacg cgtccgcctt tttcgagaac agcgtcggcc
     2521 acaatcccca tgaggccgac tcggccacct ccatagacca attcgatatt atgggtggcc
     2581 aatgcatggc cgagctctcg agcgcggaca gcgaagttgg gattatttcc aggactggca
     2641 ccacaataga cacagattct ttttattttc atcaagggaa cttaatcaga ccggacttgc
     2701 aaagaaacaa tttattcact gtttcataaa atgttgctta aaattcagtc ttcgcggcat
     2761 gctgtatcat gtatttaaaa tgtattgcca atgcgcggag cgattgttag cagttgtcgc
     2821 gaacgcaaat gcaaatataa gtcggtaatt ttggtccgac gtttctaccc gccaccatat
     2881 atggcggact atttggagga atcttgttca agttatacgt agctatcgta gcatccgttt
     2941 tttccctcaa cgccttcgct ttttcccctt ctggaaactt tccaaagggt ccagatgcgg
     3001 ttctaactcc aggagaagtc tgtgatcatc cagatgctca tcgctatcca gaagggattg
     3061 catattgcaa tagaaacgtc tcttcagacc ttaagagagc catctttgta aattatgacc
     3121 agttaggctt ccgtacaact caaatgcaaa gaatggattt caagatagat cattacattc
     3181 cactttgcat gggtggcggt aacgatatca aaaatctttg gccacagcac aaaagcatct
     3241 atgctgttac cgatcctttg gagcctcttc tttgcgataa aatggctgcc ggagttttga
     3301 ctcagaagaa agccattgaa tacatcacct atggcaaaaa tcatcttgat gaaattcccc
     3361 acatcatctc tgaagtaaat aatttgtaaa attttaagaa tctaaaaata aaaaaagcac
     3421 ggctcaatat gttgccgtgc tttttttttc tgtatatcaa caaatgacaa aatgggggtg
     3481 tttgacaatg ccgcaaaaga agaaaacaat tgcggtgcta tcccttggag gatgtcatgg
     3541 cagtaaagac tctgcgatcg gtctatgtcg ctggtgcggc tcgggtgccc ttcgtaaaat
     3601 ccgggactca atatcggaaa gttacgactc atgagctgat gcttgcaagc atgaatcatc
     3661 ttgtggctca gtatcgcctg caagaaaaag ttcttggaga tgtggccttg ggggctgtca
     3721 tgaatcgatc gacggagtgg gggattccgc gagaggtggt tttaagcact caactgcatc
     3781 cttatacgcc ggcttatttt atccaacgtg cctgcggcac ttctttggag gccacttcgc
     3841 aaattgcgct aaagatcgcc tcaggacaaa tggatacggg aatcgccgga ggttttgaca
     3901 ccaatagcga tgtcgccatc ttgtatcctc gctctttttc ccgcaagatt ttgaatttaa
     3961 gagacagtaa gagtgtaggt gcaaagcttg cgaattttct aaaattcagc cctaaagatt
     4021 tccgccttga tttgccagcg aacaaagaag accgcaccgg tttatccatg ggtgaacata
     4081 ccgagctgat ggttaaggaa tggaaaattt cccgtgaaga ccaagataaa ttgactctgc
     4141 ttagtcatct caatgcggct aaggcttatg atgaaggctt ttttaatgac ctggtttttg
     4201 aatttcaagg gttcaccagg gatggaatcg tacgtaaaga tacgagtctt gagaaattag
     4261 cgaagctgaa acccgctttt gatcgcagtc ctcaggggac cttgacggcg ggcaatagca
     4321 gccctctgag cgatggatct tcaaccgtgc tgctatgttc ggatcaaggc ctgaaggagt
     4381 tgggattcga gccctgggct cgctttgtgg atgcgcaagt ggcaggggtg gattttgttc
     4441 acggcgaggg tttattgatg gcacctgcca aagccgtgcc agtcatgctc gcaaggaacg
     4501 gactgacgct gcaggatttt gattactatg aaattcatga ggcctttgcg ggacaagttc
     4561 tttgcaccct caaggcttgg gaatcagatg aatattgccg aaaacatttg ggtttaagca
     4621 aagctttggg gtctattgat cgcagtaaat taaatattaa aggtagtagc gtcgccatag
     4681 ggcacccctt tgccgccaca ggcactcgca ttgtgggttc tctgagtaag atgctcaagg
     4741 gaacccaagg caagcgtggc ctgatttcaa tctgcacggc cggcgggatg ggagttgtgg
     4801 cgatactgga atctgtttaa gaatataaac ttaaacaaat agcctcagac actaacttaa
     4861 cggagtaaga ggcagcgccc ggttgggttg gagccttaat atccaaaaca acggtggctc
     4921 tgcaagattg tgtacttact tcgtaagtga ggttgcttat atttttaatg tttgtaatgg
     4981 gtccgtgtat tttgcctgaa agctctgttg aatgtatgac ggcttcaatt tgtcttaccg
     5041 aatcccaata aggtggcaaa acagcgaagg agtgggtcgc aaaaagcaaa gtcgaaagcg
     5101 caaaaacgta cttcataaaa attctttcat tattaagggt ttaaattcat tcctgtggag
     5161 cttctaggtg caaactggct tttaaacaag tcttaattca tgacagttgg ttccttattt
     5221 gcggatattg aaagattagt ctcttcaagc ttgctggaaa ttattttaaa gaactgatgt
     5281 tttgttttgt gctctcaaac agaaaactgt gagatttcta aaattcgttt actgatatcc
     5341 cttttaatat tttcgatctc attgtcagtg tgatctgtcc acacatagtc atcgcggatt
     5401 tttacgaggg ctcgagcttg ctgttccgtc aggggaggag aggacgacac caaatcatgc
     5461 agctgcgcat cgatttcgcg tttaaaatcc ccatattcgg tctcgttgtc gctgtggaga
     5521 gctttaagga actcttgcca tgagttttcc atggaaacct ctttttaaaa ctcaatcaaa
     5581 acatagtgaa tcttgaccct tgtgacgatt ccaggtagtt ttcttctcat tcaaaaatct
     5641 aagcttccat ctctatttat ttatcaatat tgagggccca tgattgaatc catcagtagc
     5701 tcatttcttc ttgtcgcagc cagtgaaatg ggcgacaaaa ctcaactctt agccttctcc
     5761 ttagccgctc aatataaaaa gcccttaccg atcatggccg gaatttttgt ggccaccgta
     5821 ttgaatcacg ccttggcttc ttggggtgga acctgggttg cgacgagggt gagcccattt
     5881 cttatggcca atattctggg ggggatcttt gtgggctttg ccctatggac cttaaagcct
     5941 gatgaggcgg acgaagtgga cgaaggttcg aagttcggcc ccttcctgac caccgtattt
     6001 ttattcttta tggctgaaat gggagataag acacaactgg caacagtggc tttgggagct
     6061 caatttaaat cggcttggga agtgaccctg ggcaccacat taggaatgtt ggtgacggat
     6121 ggattggcgg tcgttgtcgg agatcagctt tcgcataaaa tcaatatgaa gtatatgcga
     6181 tggtttgcgg cgagcttatt ttttggtttt ggtatcttgt gcttttggaa agcttacgta
     6241 tagatttagt ttaggcataa aaagcccatt aaaaaggggc tttttaaaat gcatcttttc
     6301 atttattaat aggtagggtg atgtttttta tttttgccct ttttggcttt cttcgaattc
     6361 tttttgcctt ttgaattcga ttttttttct tttttagatt tctttttagc ttttttcttg
     6421 gtgacctttt ttgtttcacc gagttctaaa gctggagcgg tgtgctgaat gatggcgcca
     6481 tcagagggca ttggctccaa gccaggctca ttcatcgaag gcggcgcggg aggcgcgcta
     6541 agatcagggg gtaagctctc ttgagccttc gcgaaaaacg gtaatgtcaa tgctgccgct
     6601 agtataaact tagcaaactt catgttctag attcctccaa aaagggttat ttttttagca
     6661 taagcacaaa gattcccaaa gtctctagat taaattaaaa ttgagacttc cctcggctcc
     6721 aatagttccc tcctagggag aaaggtctta tgtcttcttt gaaagtctat gaatactcag
     6781 gatgcagcac ttgtaaaaag gctttgaaat tcttggacgc tcgcaaactt aaatacgaag
     6841 ttctggccat tgtcgagaga ccaccgacga aggtcgagct tgaacggatg ttagctctgg
     6901 taaaggaaga aggaggagac cttaagaaac tttttaatac ctctggagtt ctctacaagg
     6961 agatgaaaat ttcagaaaaa ttttcgagaa tgagcgaaag cgaagctttg gatcttttgg
     7021 ctgaaaacgg caagctggtt aaacggccct tcgttttagg agctgatttc ggcctcctgg
     7081 gctttaaaga agagcaatgg aaaaaacggc tgagataaag atgtcttata acaaggtggc
     7141 ttgacgtttt tacgactgta cacatcttta ttccaggaag atccttaaag ggatcctttc
     7201 tacgaaggag atgaattatg tctattccaa ccggtttttt acccgtcctt ccgcttaaaa
     7261 atgctgtgat ctatccaggt gttggccaag ccctgagggt tggacgcgag aagagtatct
     7321 tagccttgaa taaggctcat gaaaatcaat cttggatttt agcgctggcg cagaaatcaa
     7381 tggatcaaac ccaggctctc tcggtcgaag acatttgcac ggtgggcact ctatgtaagg
     7441 tggaatctat tcgaggcagt ggggagatgg gatttcaagc tattctgcgt ggccatcaga
     7501 gaatgaaagt aaccaatttg gttttaaaag atcaatattt tcaagccctc gcggaacccc
     7561 tcgaagacct gatcgacgtt gatgagcaca cccgagcggt tttgatggaa agtttaaaaa
     7621 atcaatcacg gcaaatcttg cgttttgttc ctactaatac ggaacagctc gatgagatga
     7681 tcgagaagat tgatgacttg gggcttttat cttatttttg cgcggcgcac attgatattt
     7741 ccatgcgtga aaaacaagag cttctagaaa agaccagcct taaagaccga gtgatggccc
     7801 ttctagtgca tatgcaggat ttcaaagaaa acttgcaagt ccaggcggaa attcgcagta
     7861 aagtaagctc aaaacttggc cagactcagc gtgaacatat tttacgagag caacttaaaa
     7921 ccattcgcga agaactcggg gaagaggggc aaagttcgac caatggtgat aaatacactc
     7981 agcgcattga agaagctgga atgagcgaag aagctaagaa agtggctcag caagaagtgc
     8041 aacggctgaa aaatatttct ccacaatccc cagagtacca gatcatcagt aattatttag
     8101 atttgttgac caccttgccc tggagtaaag catcccatga gggcgagatt gatttggaaa
     8161 aagcgcgaat gattttagat gaagatcact acggtcttaa caaaattaaa aagcgcatct
     8221 tgcaatactt ggcggtgatg aagcttaaaa ataataataa aggcaacatt ttactttttg
     8281 tcggaccgcc gggggtgggc aagacctcct tgggtcaaag tatcgccaag gctttgggac
     8341 gcaaatatgt gcgtatttct ctgggaggag tgcgtgatga agcggagatc cgcgggcatc
     8401 gccgcaccta tgtgggagcc ttgcccggcc gaattattaa tagcatgaaa aaagctgggg
     8461 aaaataatcc tatttttgtt ctggatgaag tcgataagct gggacgtggg ttccaaggcg
     8521 atcccggtgc cgcattgctt gaagttttag atcctgaaca aaacaatcac tttatggatc
     8581 actatcttga agtgggttat gatctttcga acgtattttt tatcgccacc gccaacagcc
     8641 ttgagggaat tccaggtcct ttaatggatc gtttggaagt cattgaagtg agtggctata
     8701 cgacggaaga aaaattctat attgcgaaaa accatttgtt agcgaagcag ctcaatgagc
     8761 atggtatttt gcctgagaag ttggaagtca cggatgcggc tctccatcat cttatcagtc
     8821 attacacgcg agaagctgga gtgcgggagt tgcagcgaaa aatagccgct ttgtgccgag
     8881 cggctgcgga gaaagtattg agtagcactg agacagtgtt gattgacacc aaggacttgg
     8941 aagagattct ggggcccgag cgttacaccg cagaaattgc cgagaatgcg gcctctgccg
     9001 gagttgtcac tggccttgcc tggacacccg taggcgggga tattttgttt attgaaagca
     9061 cgcgtatgcc agggacaggg catctcttga tcacaggaca gttgggtgag gtgatgaaag
     9121 agtcggcgca aatcgcttta agccttttgc gatcccgatt gccgctgata aatccgctgg
     9181 tggactttag caagcaagat attcatatcc atgttcccgc aggagccatt ccaaaagatg
     9241 gtccttccgc cggcgtgact ctactcacca gtttggcgtc tttgttttcc cagcgtaaag
     9301 tggatccgaa gttggccatg actggagaga tcaccctgcg aggcgccgtg atgccggtgg
     9361 ggggggttaa agaaaaagtt ttagccgccc atcgcgcggg aatcgaaaaa attcttcttt
     9421 cgaagaagaa tgaaaaggat ctgattgagg ttccgttaga aattcggaat agcatgaagt
     9481 ttatttttgt agagaacgtg aacgaggttc taaaagcatc cttggaattg gatgtggact
     9541 tgatgggttc catttactat caaaatacgg ccctgatgca accccctcaa caaggaggag
     9601 ttggtagtaa ttaaagaaaa cggattatat ctagtcctta tcaaatcagt tcgtggaata
     9661 cgaactgatg ataatttaag aaataatctt tgcccggatc cgcacggcag tgccaattcc
     9721 gcagcttggg aattggcaaa ggcggttgct ctggaggcgg tattaaatac cgaaaacttt
     9781 ttagatgaaa ctaaaaatct agcttccgct ttgttctata cttcgaaaat aactccttac
     9841 tgggaggacg atccagattt tgttaagtac ggtcctgtaa atatcaaact taattttcct
     9901 gacttagatg acaaatcgct gaaggtgtat aatcaaaacg ttcacaatcc aaactgtgta
     9961 aagttataca gaaataaaaa atatcttgag agtatcaata aaattaaaaa tgctccgcat
    10021 aaatattatt caggaaatat catgaagtta tggaaggttc ccgctgtttt gtcttcgctt
    10081 cagatggatc gagctgattt attttagaat atccgctttg agtttctcga tgccatcatc
    10141 gctgggttgg ccgagccaaa tcgagaagat atcttgaatg aatcctggtc cgccagagat
    10201 tgcgatgatt ttggagttgg aattctcata ggtgataacc tcagaaccat cgcttttttt
    10261 agagccgagg attgacagag acttcccttt tttagcttcg ccgccaattc ggaccgcttc
    10321 taagaacttt tgaagttctg gcttcttcaa atcaacttta ttgacttcaa gggcctcttt
    10381 gaatgaagtc tgtacttttt ctccttccac gtcgcggagg aagtgaagtt gcatcatcgc
    10441 cggtgcggct tcgctgacag atttaagagc t
//
LOCUS       DATBTE010000322         4449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2023992, whole genome shotgun sequence.
ACCESSION   DATBTE010000322 DATBTE010000000
VERSION     DATBTE010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2023992"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VIG33_12110"
     CDS             <1..295
                     /locus_tag="VIG33_12110"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEY8271625.1"
                     /translation="VRLEALKENPGFDLVEDENSIESYPAEAPVDRADPLTHLRWAIC
                     RLKPIEQELILLMIDEEMSLDQMALALGLPVGTVKSHIHRAKDKLRQIMEVSS"
     gene            292..588
                     /locus_tag="VIG33_12115"
     CDS             292..588
                     /locus_tag="VIG33_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271626.1"
                     /translation="MSQKNKDLEDLLKPLKDIAPNDLQMQQWQNAVQNELTTNKAASH
                     SNIKWAFQLVAATLIGFIIGVLLFKNNSERESSSQMAQISLEDATFERSHDNLD"
     gene            614..1441
                     /locus_tag="VIG33_12120"
     CDS             614..1441
                     /locus_tag="VIG33_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271627.1"
                     /translation="MFKKLVFFILLASSLASAKEFTVQDVRERLEIHSDIYQVDGKKV
                     VGGPERTNYWRMDPEKGETEGNWSSIFNGDLIAIRHNWKVNADGTIKVTIEEFANSSK
                     EGEFSHLIEKKEFTLENLEPIVWKVKNVKDKSFIVRYIPSLREISKPIALESLPVVGT
                     GLTVSDNAGFLWVADTKEYLDGKFVGLTTHRGTIALSYVPFKGAKEMGVAEGNQINLK
                     VDKKFQINFKSETAFLPAGVIAKVYAVYLPEKKSKGVNSVHSFSSSSEERFQESLKR"
     gene            complement(1656..2699)
                     /gene="add"
                     /locus_tag="VIG33_12125"
     CDS             complement(1656..2699)
                     /gene="add"
                     /locus_tag="VIG33_12125"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY8271628.1"
                     /translation="MDSTKIYSHELRALPKVELHRHLDCSMRWSTMVELAPHVGLELP
                     QNYDTQRDFLLVTEPMKDLGAVLNKFLRVQKLLSSEEVLTRLAYEACEDAYNDGIRIL
                     ELRYAPTFIVPEHSSSLNSLNFEKVHRALQRGVEMAQKNFPMAVGMICILQRTTSLAL
                     AEKVCDFAIDHKDSFIAMDLADNEEGSDPKNFASLFKKAHKAGLHITVHSGEANIPHS
                     AQWVKDSIEILGAERIGHGIQIIKDLNILHFVRDQGIPLEVCPISNWLTQAFKTHEEH
                     PIRALMNAGIKVTINADDPGIFSTQLSDDYEVLHRVHGFGIADFEKANDIAASASFID
                     YKEKQKYWPRKIL"
     gene            2783..>4449
                     /locus_tag="VIG33_12130"
     CDS             2783..>4449
                     /locus_tag="VIG33_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEY8271629.1"
                     /translation="MTEPIKITNKLAGNPLQEPQFKTFISVNDGTKIPAVDPRATRAL
                     ISLMDMNAVLGGAASHYGGPAAMAELMSALHAHVFLEAKLAGKEWHELYNLVNDAGHC
                     ENGLYALKANYGMAALNLNALKKFRSIESPLTGHGEVHCFPQGVFISNGPLGSAFPQT
                     QGVSIGDALAGRNRVTVTVVSDGASMEGEAKESFAAIPGLAKAGKMAPYVMIISDNNT
                     KLNGRIDSESFSMAPTFESLKNLGWNVIELEKAHDLQACYNVLVKAIDAARKNPKQPI
                     AVHAKTIKGIGTKKTAESANGGHGFPLKSPTELPGFLSEIYNGEQFPAEFTVWIEELI
                     KLEAQMKAAAKESGAKDAGEKIQNGVASALIRARKENLPVISVTSDLPGSTGLAAFRK
                     EFPADSLDVGVAESNMVSTAAGLSKVGYIPVVDTFAQFGVTKGALPFIMSSLSEAPVI
                     AIFSHTGFQDAADGASHQSLSYMAMLASIPHVETYALTCSEEADALVYQAVCKTAEAR
                     RNGKVPPSYVFFLGRENFPKSYTAGLKYELGRGQVLFDNTDKFPTSIT"
BASE COUNT         1263 a          892 c         1049 g         1245 t
ORIGIN      
        1 tgtaaggtta gaagcattaa aggaaaatcc tggatttgat ctggtggagg atgagaactc
       61 catagaatct tatccagcgg aagccccagt ggaccgcgcg gatccgctga cccatttgcg
      121 gtgggccatc tgcagactca agccgataga gcaagaattg attcttttaa tgatcgacga
      181 agagatgtcc ttagaccaaa tggccttggc gctaggttta cctgtgggga ccgtaaagag
      241 tcacattcat cgtgccaaag ataaattacg acaaattatg gaggtttcgt catgagccaa
      301 aaaaataaag atttagaaga tctactgaaa cctttgaaag atattgcccc caacgacttg
      361 cagatgcagc aatggcaaaa tgctgtgcaa aacgaattga cgacaaataa ggcggcttct
      421 cattccaata taaagtgggc tttccaattg gtggctgcca ctttgatcgg attcatcata
      481 ggcgtattgt tatttaaaaa taattccgag agagagtctt cctcacaaat ggcacagatt
      541 tccttggagg atgcaacttt cgagcgttct cacgacaatc tagattaaac aatttatttt
      601 ttttggagat tatatgttta aaaaattagt tttttttatt ttattagctt cctcccttgc
      661 atctgcaaaa gaattcactg ttcaagatgt tcgagagcgc cttgagattc attcggatat
      721 ttatcaagtg gatggcaaaa aagttgtcgg aggacctgag cgcaccaatt actggcgtat
      781 ggatccagaa aaaggggaaa cggaagggaa ttggagttct atttttaacg gtgatctgat
      841 agcgattcga cataattgga aagtgaatgc ggatggcaca ataaaggtga ccattgagga
      901 gtttgcgaat tcctctaaag aaggtgagtt ctcgcattta attgaaaaaa aagaatttac
      961 cctagagaat cttgaaccta tcgtctggaa ggtaaaaaat gttaaagata agagctttat
     1021 cgtccgttat attccatcct tgcgtgagat ttcaaaaccc attgctctag aaagccttcc
     1081 tgtggtcgga accggattga ctgtttctga caatgctgga ttcctgtggg tggcagatac
     1141 taaagaatat ttagatggaa aattcgttgg cctgactact catcgcggaa ctattgcttt
     1201 gagctatgtt ccgttcaaag gagctaagga aatgggtgtt gctgaaggga accaaatcaa
     1261 tttaaaggtt gataaaaaat ttcaaattaa ttttaaatct gagaccgctt ttttacctgc
     1321 tggtgtaatc gcgaaagttt atgccgtcta cttgcccgaa aaaaagagca agggagtaaa
     1381 ctcggtccat tctttttctt caagttcaga agagcgtttc caggaatctc ttaagcgata
     1441 gagggaaatg gcgctaatac tgcctttggt atcagtggca taaaatgcaa cttaatggca
     1501 tattatgcca cttggatttg agaaagctga atagatcatt cctggacagc cgttgattgt
     1561 ttatttgagc cagaaggcag gggttgacta caacaccgag gaatggcttg aaaaataaga
     1621 ggaacgaaat ctatctaaag aactataata taaaactata gaatttttct tggccaatat
     1681 ttttgtttct ctttgtaatc aataaaactg gcggaagccg caatatcgtt agccttttca
     1741 aaatcagcta tgccaaagcc atgcacgcga tggagaactt catagtcatc cgaaagctgt
     1801 gtgctgaaaa tgccgggatc atctgcattg atcgtgactt taatgcccgc attcatcagc
     1861 gcgcgaatgg gatgctcttc gtgggttttg aaagcttggg taagccagtt gcttatcggg
     1921 cagacttcca gcggaatgcc ctgatcgcga acaaagtgaa gaatgttgag atctttgata
     1981 atttggatgc cgtggccaat acgctcggct cccaaaattt cgatggagtc tttgacccat
     2041 tgtgcggagt gaggaatatt ggcttcgcca gaatgcacgg tgatatgcag cccggctttg
     2101 tgggctttct tgaaaaggct ggcgaaattt ttaggatcag aaccttcttc attgtcagca
     2161 agatccatag caataaagct gtctttgtga tcaatcgcga agtcgcaaac tttttcagcc
     2221 aacgcgagag aggtagttct ttggagaata caaatcatcc ccacagccat tggaaagttt
     2281 ttctgagcca tctccacccc cctttgcagg gctctatgaa ctttctcgaa attcaaagaa
     2341 ttaagagagg aactgtgctc aggaactata aaggttggtg catagcgaag ttcgagaata
     2401 cgtatgccat cgttataggc atcctcacag gcctcatagg ctaaacgggt taagacttcc
     2461 tccgaactca aaagtttttg cactcgaaga aatttattta ggacagctcc cagatccttc
     2521 atgggctcag tgactaacag gaaatccctt tgagtatcat aattttgagg taactcgagg
     2581 ccgacatgcg gagctagttc caccatggta ctccaacgca ttgagcagtc taagtggcga
     2641 tgcagttcta ctttgggaag tgctctgagt tcatgggaat aaatttttgt tgaatccatg
     2701 gtcttagagt tatcattctt gctccgtcct aggcatctct ttttgttgac gcaaatatgt
     2761 attctagagg aggatgctgc ctatgacaga accaataaaa atcactaata aactcgctgg
     2821 aaatcccctc caagaaccac aatttaaaac tttcatttcc gtaaatgacg gaacgaagat
     2881 cccggcggtg gatccccggg ctacgcgtgc attaatttct ttaatggaca tgaacgcagt
     2941 tcttggaggt gcggcctctc attacggcgg tcctgcggcg atggctgaat taatgtccgc
     3001 tcttcacgct catgtttttc tcgaagctaa acttgcaggg aaagagtggc acgagcttta
     3061 caacttggtg aacgatgcgg gtcactgtga aaatgggttg tacgccttaa aagcaaatta
     3121 cggcatggca gctttaaatc tgaatgcgct taagaaattc cgttctattg aaagtccatt
     3181 gacaggccat ggagaagttc attgcttccc gcaaggtgtt ttcattagca acggtccttt
     3241 aggttctgcc tttccacaaa ctcaaggtgt ttctattggc gatgctttgg ctggccgcaa
     3301 ccgtgtgaca gtgactgtgg tttctgacgg cgcaagcatg gaaggagaag cgaaggaatc
     3361 ttttgccgct attccaggtc tggctaaagc cggtaaaatg gctccttatg tgatgattat
     3421 cagtgataac aacacgaagc tcaatgggcg tattgattcc gaaagcttct cgatggctcc
     3481 cacttttgaa tctttaaaaa acttgggttg gaatgttata gaattagaga aagctcacga
     3541 tctacaagct tgttacaatg ttttagtgaa ggcgatcgat gccgctcgca aaaatccaaa
     3601 acagcctata gccgttcatg caaaaacaat caaaggaatt ggtactaaga aaacagcgga
     3661 gtccgcaaat gggggtcacg gtttcccatt gaaaagtccc acggaattgc caggcttttt
     3721 gagcgaaatc tataatggtg aacaattccc cgcagagttc actgtctgga tcgaagaact
     3781 gattaaatta gaagctcaaa tgaaagccgc ggcgaaagag tccggcgcaa aagacgccgg
     3841 agagaaaatt caaaacggag tcgcgagtgc gttgattcgt gcgcgtaaag aaaacttacc
     3901 ggtgatttcc gtaacttcgg atctccctgg ttctacgggg cttgcggcat ttcgtaaaga
     3961 atttccagca gattctctag atgtgggtgt tgcggaaagt aacatggttt ccactgcagc
     4021 gggactttct aaagtcggtt atattccagt ggtagatact ttcgctcagt tcggtgtcac
     4081 caaaggggct ttgccgttca ttatgagttc tttatccgag gccccggtga tcgcgatctt
     4141 ctcgcatacg ggtttccaag atgcggccga tggggcttct catcaaagtt taagttacat
     4201 ggcgatgttg gcttcaattc ctcatgttga aacttatgca ttaacttgca gtgaagaagc
     4261 ggatgctttg gtctatcaag cagtttgtaa aacggcggaa gctcgccgta atggtaaagt
     4321 gcctccttct tatgtgtttt tcttaggacg ggagaatttt ccaaaatcat atactgcggg
     4381 tctcaaatat gaacttggtc gtggtcaagt cctctttgat aatacggaca agttcccaac
     4441 cagtattac
//
LOCUS       DATBTE010000323         4694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_726515, whole genome shotgun sequence.
ACCESSION   DATBTE010000323 DATBTE010000000
VERSION     DATBTE010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726515"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VIG33_12135"
     CDS             complement(<1..665)
                     /locus_tag="VIG33_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271630.1"
                     /translation="MSNQKNNENSEFHSEFYSGIYQVKLDSISLFFDETPLLKGKSLK
                     NLLNLNFSKYKLHELAPDIGSKDPEFAAPWLWSMPEVFRKKLTAYSEYYFNEVNKQNI
                     LADVVRYNFSNGMQLSSKEILRHTLQEQVPRGLLPQENRAHSMTWSLPWPLLQQQDNS
                     FVSQTVVDIEGIVQALLDKVVAWHRQGKLDLYLQTRSHPQGALRIQAVEMDPATEVNW
                     RVD"
     gene            complement(738..1541)
                     /locus_tag="VIG33_12140"
     CDS             complement(738..1541)
                     /locus_tag="VIG33_12140"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEY8271631.1"
                     /translation="MKRLQFLFLIVASLAFVSCNSKNHFDREITAQTFQSRAGIVNGD
                     RVKKYDPVATHVVAITDQAMENCTGTLIAKNLVLTAAHCQTPDEKMYIAFGLEVSPDN
                     IQKINLHEVTAYRMAPGYQDVLTSFKEVDHADLMILQFDGEAPAGYEAAEILNDASVL
                     FEGMFVTVAGYGVTDGIEQEGDGFLRKTQVSIANPSYARTEVSTDETMHGSCSIDSGG
                     PAFVEVDGKTYIWGVTSRGDVNCRKRGVYTRIDSYLPWVDAVIKELSPL"
     gene            complement(1672..3402)
                     /locus_tag="VIG33_12145"
     CDS             complement(1672..3402)
                     /locus_tag="VIG33_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY8271632.1"
                     /translation="MKMNVLRALVLPWLFLFVVIIAAIVSDGMNFRFQQQYPHYVWYV
                     LWVGVAVCLYANFATLSKAGIWIAYSLPSSLKNSPSAWILRGVLVALVAFGFYMAGQT
                     PWAPVVWQAAVVPGISAICLFILIRSLMGPILVWCSRVAFSRFSAFVLSIPIFALVPI
                     TAIFLGNMIFNAYQASRPDFVMVPQKASAIEKTPREVAGSEVVENKFEVKAKDKLAME
                     FQEAVQSGKDCSDLHKEIQQALTPNGPENLVYWAVRATKCTEMKSVVGLPKLAKIMTD
                     HPNALVRAAAIRAMMKFPHENVKRIGYLIVKRISEHEPLEVIEAASVALPKLGNEEAL
                     WTVKRLKNLLDSPKASGMASMVLVKNLKREDLVAEYVSGNLGSDDSPASKRAISMICS
                     LPKESRKMAEPHIESIVAAIKTGEANDPAIDALECLGHPGFLAIRQELMAPHRLDKPI
                     AARALAEMDVKTEAPDATLKIAVTCARDGNSEVRRWCSQTLGKIGAAALPQILDLLKS
                     SDKGLKEAGTNALNFFKDPIAKQELVKIRAENSGWMANQKKLQIAKAVDRALIKIISE
                     ETVGQNHETR"
     gene            complement(3481..>4694)
                     /locus_tag="VIG33_12150"
     CDS             complement(3481..>4694)
                     /locus_tag="VIG33_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271633.1"
                     /translation="YTNENLRNRALEIINSSWQVRLGIHRKLGEVDKECILLARDIFH
                     SLRDAEDYLGDFAYSVRKVYPADLDFQKQPVPIYDRQAYPPYFVRPDLDDPKFNFHAG
                     DLMLARGVSFTSAIISQISDNRSHFSHVVFVDVNAKTQAPSTIESYVGSGVKPYEINF
                     ALKNENARLLVLRPKDTALGERAAIFANQAASTRVPYDYAMNFKDYSALSCVELSIYS
                     YDKASQGTLKLPLYPAQLNLDNRDFLHKMGLKKGTLVTPDDLETDPNFDMVLDWKDYR
                     LIRDSRHRDAILSEMMRWMNDLKYTFHNTFDSVVAEYVVHPTRPTPLWPLVQKLTGAP
                     DIDKEIPEQTLGVMTVLNQVGNALLVELRKRDEAYVFRYHRPMTNKQLRDALEGMRRA
                     DLKSIHNTFHP"
BASE COUNT         1315 a         1154 c          998 g         1227 t
ORIGIN      
        1 aaatccacgc gccaatttac ttccgttgcg ggatccattt caacggcttg aattcgcaaa
       61 gccccttggg gatggctcct cgtttgtaag taaagatcga gtttcccctg gcggtgccaa
      121 gcgaccactt tatctaataa agcttgaaca attccttcaa tatccacgac tgtctgacta
      181 acaaagctat tgtcctgttg ctgcagcaat ggccacggca gcgaccaggt catggagtgg
      241 gctctatttt cctgcggcaa aagcccacgg gggacctgct cttgcaaggt atgacgaaga
      301 atttcctttg agcttaactg catgccatta ctgaaattat aacggacgac gtcggctaag
      361 atattttgtt tattcacttc attaaaataa tattctgagt aagcggtgag cttcttgcga
      421 aacacttcag gcatgctcca gagccagggg gccgcaaact ctggatcttt gcttccaata
      481 tctggggcta attcatgaag tttatattta ctaaaattaa gattgaggag atttttaaga
      541 gactttcctt ttagtaaagg agtctcatca aaaaagaggc tgatagaatc gagcttcact
      601 tgataaattc cagaataaaa ttcagaatga aattcagagt tttcgttatt tttctgattc
      661 gacaccggga aattttgccc gaggcttaac aagttgtcac gggccaaagg catcggcccg
      721 gattataaaa atagaattta gagaggactc aactctttaa tgaccgcatc gacccaaggc
      781 aaataggaat caattcttgt gtaaactcct cgtttccggc aattcacatc gccccgactt
      841 gtcactcccc agatataagt ttttccatcc acttcaacga aagccggccc cccagaatca
      901 attgagcatg agccatgcat tgtttcatcg gtggagactt cagttcttgc ataagatgga
      961 ttggcaatgg agacttgagt ttttctcaaa aatccatcgc cttcttgttc aattccatcc
     1021 gtaactccgt acccggctac ggtcacaaac attccctcaa ataaaacgga agcgtcatta
     1081 agaatttccg ccgcttcata gccagccgga gcttctccgt cgaactgaag gatcatgaga
     1141 tccgcatgat ccacttcttt aaaagaggtc aagacatctt ggtatccggg agccatccgg
     1201 taagctgtca cttcatggag atttatcttc tggatgttgt ctggggaaac ctcaagacca
     1261 aaagcgatat acattttttc atcaggagtt tgacaatgag ctgcggtcaa aactaaattt
     1321 ttagcgatca aagttccagt acaattttcc atggcctgat ccgtgatggc aacaacatgg
     1381 gtggcaaccg gatcatactt tttaaccctg tcaccgttta caataccagc tctcgactga
     1441 aacgtttgag ccgttatttc ccgatcgaaa tgattttttg aattgcaact tacgaaagct
     1501 aaagaggcca cgattaaaaa taaaaattga aggcgtttca ttcattcccc cctcgaataa
     1561 ataaaaatag tggacgcact ttcaatgaat tctcttttaa attcaagatc aatccttgcg
     1621 gagtaaatta tacaaattta ctccgcaagc cagtgccagc agaatccagc actatctcgt
     1681 ttcgtggttc tgccccacgg tctcttcgct gattatttta atcaaggctc tatcaacagc
     1741 tttggcaatt tgcagttttt tctgattcgc catccagccg gagttttcgg cacgaatctt
     1801 aaccaattcc tgttttgcaa tggggtcctt aaagaaattc aaagcgttgg ttccagcctc
     1861 tttgaggccc ttatcgctgg atttcaaaag atctagaatt tgcggcaagg cagcagcccc
     1921 aattttaccg agggtctggc tgcaccatct tcttacttct gagtttccat cacgggcgca
     1981 agtaacagca attttaagtg tcgcatccgg cgcctccgtc ttcacatcca tctctgccaa
     2041 agcacgggcc gcgatgggtt tatcaagacg gtgaggtgcc atgagctctt ggcgaatagc
     2101 taagaaccca ggatgaccta agcactccaa agcatcaatt gccggatcat tggcctcgcc
     2161 tgttttaatc gcagccacga tactttcaat gtggggctcc gccattttac ggctctcttt
     2221 gggcaaagag cagatcatgc tgatggctcg tttacttgcc ggagaatcat cactacccaa
     2281 attgccagac acatattcgg ccaccaaatc ttcgcgtttt aagtttttca ccaaaaccat
     2341 cgaagccatg ccacttgctt tagggctgtc caaaaggttt ttgagtcttt ttaccgtcca
     2401 caaagcctct tcattcccaa gttttggcaa tgccacagac gccgcctcta tcacttctaa
     2461 aggctcatgt tcggaaatcc tttttacgat gaggtaaccg atacgcttca cgttctcgtg
     2521 agggaatttc atcatagcac ggatggccgc agcccgaacc aaagcgttcg ggtgatcggt
     2581 cataattttc gcaagctttg gcagacccac gacagatttc atctcagtgc atttcgtggc
     2641 ccgaacggcc cagtagacca gattttcagg cccattagga gtcagagctt gctgaatttc
     2701 tttgtggagg tcggaacaat ctttgccgga ttgaacggct tcttgaaact ccatggccaa
     2761 cttatctttg gccttcactt cgaatttatt ttccacgacc tcgctcccag ccacttctct
     2821 gggggtcttt tctattgcag aagctttttg cggaaccatg acgaagtccg ggcgactggc
     2881 ctgataagca ttgaaaatca tattccctaa aaaaatagcg gtaataggaa ccagggcgaa
     2941 tatcggaatg cttaaaacaa aagcggagaa tcggctgaag gcaacccgag agcaccatac
     3001 taagatggga cccatcaggc ttcttattaa gataaataaa cagattgcag aaattcccgg
     3061 gaccacagca gcctgccaca ccacaggagc ccacggagtc tgccctgcca tgtaaaagcc
     3121 aaaggcgacc agagccacta gcaccccacg gagtatccag gcagagggag agttcttcaa
     3181 agagctaggt aaggaataag caatccaaat tcccgctttg ctaagagttg caaagttagc
     3241 atagagacac accgccactc cgacccataa aacgtaccaa acatagtgag gatattgttg
     3301 ctgaaaacga aaattcatcc catcgctgac gatcgctgcg ataatcacca caaataaaaa
     3361 aagccatggc aaaaccaatg cccgcagcac attcatcttc atgagacctc cagtccaagg
     3421 ccccaagata aaatgcgcta ggacctaggt ccagcattcc tagaattttg acgcactact
     3481 ttatggatga aatgtattat gaatggattt tagatctgcg cggcgcattc cttctagagc
     3541 atcccgcagt tgcttattgg tcatgggccg atgatatcta aaaacatagg cctcatcccg
     3601 tttgcgaagc tccactaaaa gggcattccc cacttgatta aggacagtca taacccccaa
     3661 ggtctgctca ggaatctctt tgtcgatatc cggagcccct gtgagctttt gcaccaaagg
     3721 ccacagagga gtgggtcgcg tggggtgaac cacatactct gcaaccacgg aatcgaaggt
     3781 attatggaaa gtgtacttta aatcattcat ccagcgcatc atctcagaca aaatagcatc
     3841 tctatgccgg gaatcgcgga tcaaacgata atccttccaa tccaatacca tgtcaaaatt
     3901 gggatctgtt tccaaatcat cgggagtcac caaagtccct ttcttgagtc ccattttatg
     3961 gaggaaatcc ctattatcga gatttaattg ggcaggatag aggggcaact tcaaggtccc
     4021 ttgagaggcc ttatcataag aataaatcga aagttctaca caggagagcg cactgtaatc
     4081 tttaaagttc atagcgtaat catagggtac tcgcgtagaa gccgcttgat tcgcaaaaat
     4141 agcggccctt tcccccagcg cggtgtcttt gggtcttaac accagaagtc gtgcattttc
     4201 attcttcaag gcgaaattga tctcataagg ttttacacca gagcccacat aggactcaat
     4261 ggttgaagga gcctgagttt tcgcattcac atccacaaag accacatggc tgaagtgcga
     4321 acgattgtca ctgatctggg aaataatggc gctggtaaac gaaactccac gagccagcat
     4381 caagtcgccc gcgtgaaaat taaatttagg atcatcaaga tccggacgca cgaaataggg
     4441 aggataggct tgacgatcat agatcggaac tggctgcttt tgaaaatcta aatcggcggg
     4501 atagacctta cgaacggagt aagcaaagtc cccgaggtaa tcctcagcat ctctcagact
     4561 atgaaaaata tctcgcgcta aaagaatgca ttctttatcg acttctccca gcttacgatg
     4621 gattcccagc ctcacctgcc aagacgagtt gatgatctcc aaagcccggt tccgtagatt
     4681 ctcattggta taag
//
LOCUS       DATBTE010000324         1826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1272054, whole genome shotgun sequence.
ACCESSION   DATBTE010000324 DATBTE010000000
VERSION     DATBTE010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272054"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..608)
                     /locus_tag="VIG33_12155"
     CDS             complement(225..608)
                     /locus_tag="VIG33_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271634.1"
                     /translation="MKLNLKTVVLFFFTLSGLRQAYALTNEICTDQSGRIKIVIQQDL
                     NNKIRHIELYEDAQLRVRDTVGAQYCHVDGAIYYGKIFSYGGCYNWGDTPARFLSWDK
                     FFRSNSERTSDEITVDLNTFNCVFQ"
     gene            complement(605..1066)
                     /locus_tag="VIG33_12160"
     CDS             complement(605..1066)
                     /locus_tag="VIG33_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271635.1"
                     /translation="MKLNFTKTFLATIGLGVLSLEQVHADQLVLSCKETLGNSTYLFE
                     VYEGGFDDKLSLKEITVDGQVISSIMVPATGGSFVGCGTRTSTCNMIQSTQYSSNGPN
                     IIFTMLGTPDSKKGHGTLNMYENGFGEEAEATKTIEFDCQKKQLYVTKGEL"
     gene            1305..>1826
                     /locus_tag="VIG33_12165"
     CDS             1305..>1826
                     /locus_tag="VIG33_12165"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8271636.1"
                     /translation="MNLNSSQLAAFFAIAKSMNFTKAAEQLHVTQSALSQRITKLEED
                     LGTTLFIRDRTAIRLTEAGARVLRYCQLNQMAEAELLAKLKSSNEDLAGVLRVGGFSS
                     INRSLVIPALKKIMNKNPRFSIQLLTKEIRELSRLLITSEADYIFATNKSSSEDIETI
                     FLGYEDNVQVKLKR"
BASE COUNT          534 a          375 c          326 g          591 t
ORIGIN      
        1 gacttcattt ttgaacgcat gaagtgacgt gtttcaaatt cacttgtttc aaacgaaatt
       61 gccgctcgcg aattcgagcg gcaaaagtct aggttcttaa aaatattttc actgaatcaa
      121 aaggctcatt caaatgatca ccgctgacta gagagcagtg ttagttgatc cctattaatt
      181 cccaataata tcattaaaga cagcctttcc ctccggactg tgtcctactg gaaaacgcaa
      241 ttgaatgtat ttagatccac agttatttca tctgaggttc tttcagagtt cgatctaaaa
      301 aacttatccc aagataaaaa cctagctggt gtgtcgcccc aattgtagca gcccccgtat
      361 gaaaaaattt ttccataata tatagctcca tcaacatggc aatattgtgc cccgactgta
      421 tctcgcacgc gtaattgtgc atcttcgtat aactcaatgt gccttatttt attgttgaga
      481 tcttgttgaa tcacgatttt tattcttccg ctttgatctg tgcaaatttc attagtcaaa
      541 gcgtaagcct gtctcaaacc agacaaggtg aaaaaaaaca aaaccacagt ctttaaattt
      601 aatttcataa ttccccctta gtgacataaa gttgtttttt ttgacaatca aattcgatag
      661 tttttgttgc ctctgcttct tctccaaagc cattttcata catattaagg gtcccgtgac
      721 ctttcttaga gtctggtgtt cctaacatcg tgaaaatgat attagggcca ttacttgaat
      781 actgagtact ttgtatcata ttgcaagtgg aagttcttgt tccacaccca acaaagctgc
      841 ctcctgtggc tggcaccatg atggaagata taacctgccc atctactgtg atttccttta
      901 gagataactt atcgtcgaag cctccttcgt agacttcaaa tagatatgta ctattaccaa
      961 gagtttcttt gcaggaaaga accaattgat ctgcatggac ctgttcaagg ctcagtaccc
     1021 ctaacccaat agttgcaaga aaagtttttg taaaattcaa tttcataaga tctcctttgt
     1081 taattctttc gtaaaatatt gttaacagtg ccatttatcc attgaatgcc gataagtctg
     1141 ggcttctgcg gcatctattt tcgtcagtcc taatttttga ctgcgcttgg tgattcttct
     1201 agcaaatgtt tcccatacgt ttctctcgaa tgtctttcta tgacgaaagg agccttgtgt
     1261 aaaattaatt gattttatga tataattaga atatctaatg atccatgaat ctgaattctt
     1321 cacaacttgc ggcctttttt gccatcgcaa aaagtatgaa ttttacaaaa gcagctgaac
     1381 agttgcatgt cacccagtca gctctttccc aaagaattac taaactggag gaggacttgg
     1441 gaacgactct ttttattcgt gatcgaacag caatccgcct taccgaagct ggcgcaaggg
     1501 ttctacgata ttgtcaacta aatcagatgg ctgaggccga gctgcttgca aagcttaaat
     1561 cttccaatga agatttggca ggtgttttaa gagtgggcgg tttttcatcc attaatcgct
     1621 cgctagttat tcctgcgctt aaaaaaataa tgaataagaa tccgaggttt tccatacaac
     1681 ttttaactaa ggaaatccgg gaactttcaa gactgttaat cacttctgaa gctgactata
     1741 ttttcgccac gaacaagtca tcgtcagaag atatagaaac tatttttttg ggatatgaag
     1801 acaatgttca ggtgaagcta aaaagg
//
LOCUS       DATBTE010000325         1965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_795579, whole genome shotgun sequence.
ACCESSION   DATBTE010000325 DATBTE010000000
VERSION     DATBTE010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795579"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1718
                     /locus_tag="VIG33_12170"
     CDS             <1..1718
                     /locus_tag="VIG33_12170"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEY8271637.1"
                     /translation="VVVRWIPWKDAGSAYATIQPNPLTGEILRGQIFLPSSWLNPELI
                     GESMSATGLAKGGLGCEVGATAAKDIALLTQTVSKETRQAILDYLRYVAAHESGHTLG
                     MRHHFSAASQVKASFEEIHQATETYRKNPAQQVQYPELSISVMDYMLPLDSAIIGHSL
                     MTRALSYDQDLIEWAYNGKEKLLSTAGAFCTDDHLMYAGEEGLSIMGCGRFQNVGSPL
                     MAQLKAISNRDDASIESDVKTIRNSSISEDGKFKALAVDQYYQGLAAYTISGRTNGLD
                     NLLFSIKNEKDNSEVNLLLLPHEVTKILLKKRFFSGQTSTEVRDKLTEQIKEVTDFPS
                     RLRLILPMDEQGKIDLNWAKNQLEKIDLKKYQQGKTSSGVEYQLSDDEFTQLQAAIQE
                     TIRYDERAKLIHSALRLLVDKKGIKSSLMSEKDASEKAVTVSYRDWVANSKIKELLKP
                     YIAQLLTLDLGPKKIADVKGQAVYGKFYVVNKSAFESLQSAVQGSNLILGITLQPIVA
                     QRARDISEVLAAFGFTLNLEASTEEQIKAVDESSYSGQLDFSIAYWAKEEIEFNKALL
                     EQGSL"
BASE COUNT          582 a          375 c          486 g          522 t
ORIGIN      
        1 ccgtcgtggt tcgttggatt ccgtggaagg atgcgggctc ggcttatgca acaattcagc
       61 caaatcctct gacgggcgag attctgcgtg ggcagatctt tttgccttcg agttggctga
      121 atcctgaact cataggggaa tcgatgagtg caacaggatt ggcgaagggt ggacttggct
      181 gtgaagtcgg tgctactgcg gcaaaggaca tcgctctttt aacccagaca gtgagcaaag
      241 agacccgcca ggctatcctt gattatttgc gatatgtcgc cgctcacgaa tccggtcata
      301 ctttgggaat gagacatcac ttttccgcag cctcacaagt gaaagcaagt tttgaagaga
      361 ttcatcaggc gactgaaacc tatcgaaaga atcctgctca gcaggttcag tacccagaac
      421 tcagtatctc ggttatggat tatatgttgc ctttggattc agcgattatc ggtcatagtt
      481 taatgacccg tgctcttagt tacgaccaag atctgattga gtgggcttac aatggaaaag
      541 aaaagctctt atccactgcg ggagctttct gtacggacga ccacttgatg tatgccgggg
      601 aagaaggatt gagcatcatg ggctgcggaa ggtttcaaaa tgttggaagc cccttgatgg
      661 cccaattgaa agccatcagc aacagagacg atgcgtccat cgaatcggat gtaaaaacaa
      721 ttcgcaacag ttcaatttct gaagatggaa aattcaaggc tctggctgta gaccagtatt
      781 accaaggctt ggcggcctat acgatctcag ggcgtaccaa tggcttggac aatctgctat
      841 tttcgattaa aaatgaaaag gacaattctg aagtgaattt acttttgtta ccccatgagg
      901 tgacaaaaat tcttttaaaa aagcgattct ttagtggtca aaccagcacc gaagtccggg
      961 ataaacttac tgagcagatt aaggaagtga cggattttcc ttcgaggcta agactgattt
     1021 tacctatgga tgagcagggt aagatcgact tgaactgggc taagaatcaa ttggaaaaaa
     1081 tcgatttaaa gaagtatcaa caaggaaaaa cctcgagtgg ggttgaatat caattaagcg
     1141 acgacgaatt cactcaactg caggctgcca ttcaagaaac cattcgctat gatgaaagag
     1201 ctaagttgat tcattcggcc cttcgtttat tggtggacaa gaaagggatt aagtcgtctc
     1261 tgatgagcga gaaagacgct tcagaaaaag cggtcaccgt gtcttatcgc gattgggttg
     1321 ctaatagtaa aatcaaagaa ttgctgaaac cttatatcgc gcaattactg accctggatt
     1381 taggccctaa gaaaattgcc gatgttaaag gtcaagctgt gtatggaaaa ttctatgttg
     1441 taaataaaag tgcgttcgaa tctctgcaaa gcgctgtgca ggggtctaat ctcattttgg
     1501 gaatcacgtt gcaaccaata gtggctcagc gtgctcgaga tatcagcgag gtgcttgctg
     1561 cattcggttt tactctgaat cttgaggcct cgacggagga gcaaatcaaa gcggtggatg
     1621 agtcgtcgta tagtggacaa cttgatttca gcattgccta ttgggccaaa gaagaaattg
     1681 aatttaataa ggcactgttg gagcagggga gtttgtaagc cgttcgatcc gagcttctgg
     1741 ctatagtgaa taggtaatca ataataatat attatagcat atctttatcg tgctaataag
     1801 tatactgcag tataatatat gtatacataa tgatatcata tagtatatca tgttctcatg
     1861 aaaaagagca aagccctggt taaaactcaa cgaaaaagtc tggatgaaaa gcttcggact
     1921 ttcacgcagg tcaagatgtt gataaatcca aaggctggat gggtg
//
LOCUS       DATBTE010000326        42010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1272498, whole genome shotgun sequence.
ACCESSION   DATBTE010000326 DATBTE010000000
VERSION     DATBTE010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 42010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42010
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272498"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..605
                     /locus_tag="VIG33_12175"
     CDS             258..605
                     /locus_tag="VIG33_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271638.1"
                     /translation="MKKINFLIMSLVIVAGTGVSSKGFAYGGSHLVWICSGQYQIFSD
                     HVEQKIITGSELKKIVSEHLDKKIQSPSYVICPRSTGGGYNFLLTLFGQEENHTLVDS
                     YIVSGEDLKKFME"
     gene            773..1312
                     /locus_tag="VIG33_12180"
     CDS             773..1312
                     /locus_tag="VIG33_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271639.1"
                     /translation="MNYKLYLKVVSVTFLLGFVNFSWAVDVQNGSSSFLSDSLTKMIQ
                     LFANDKTYDELKLRLWNEYLSYSERYAEKFAEVAKENQTSLKSYMCASGNPSESAKKI
                     QSFQRTTVNDFNLKNDRDQIEIAKLQQLQATYKEICEASGDGLALNNKASMVIAETTF
                     YSIYISTRDQLIAFLNLKN"
     gene            complement(1347..2243)
                     /locus_tag="VIG33_12185"
     CDS             complement(1347..2243)
                     /locus_tag="VIG33_12185"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8271640.1"
                     /translation="MKLNGIDLNKLYVLCSVVQHGGYKGASEDLGLSRSAISQSLTGL
                     ESQLGYALFHRVGTKLVLTQTAQQFHDNIARSLLEIENSVSQFTNPQTESSGTLRIGS
                     YLEFTKSKMMPVLQKFLETEPRVQIKFSFHSPSRLLLALEQQKLDLCISIFPYEGRKS
                     SITSRKLYQEELVLIARKDQWSENPEREKIMMAPVIDYYPNHVLFRRWWKCHYHQNLA
                     KVNLRCYAATADMVVEFVKRGLGVGVVPKYVFELGNKDNLMHILKPSNRRLLDYVWLI
                     YFKQPRSSHLQNKLLKLLESID"
     gene            2327..2968
                     /locus_tag="VIG33_12190"
     CDS             2327..2968
                     /locus_tag="VIG33_12190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HEY8271641.1"
                     /translation="MNEITSLIQTFHLLKHPFYQKWMAGELTQENLQNYTLQYYPHVK
                     AFPRFVSAIHSHCEDLHSRKILLENLLDEEGYPNALPHPQLWKDFGQGLGLEDSDFEQ
                     KPIGPKAQEMVECFDRLTKKSYASGLGALYAYESQIPEIAAAKIQGLKERYQIEDPAT
                     LAFFTVHETADKYHSESCAKLIESLSEQEKAEACASTREACLSLWDFLTEVNV"
     gene            2965..3321
                     /locus_tag="VIG33_12195"
     CDS             2965..3321
                     /locus_tag="VIG33_12195"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY8271642.1"
                     /translation="MILEIHRLMLKVRLGCTAGERLHPQDVAVSVSMTFAHGVQALES
                     DQLSETLCYQEICQMLKTVAMAKEYNTVEHLAFQMSGVLKPLLRSLASAELSVHKIAP
                     PIEDLTGGVTVRLTVT"
     gene            3333..4598
                     /gene="folP"
                     /locus_tag="VIG33_12200"
     CDS             3333..4598
                     /gene="folP"
                     /locus_tag="VIG33_12200"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY8271643.1"
                     /translation="MRARVFLSLGANLGDRKQTLHKAVQLLPLEDKKLSPLFETPALL
                     PSNADSWNIPFLNLVVEGTTEAPAEQLLETLKGIERDLGRKPAARWAPRLIDIDILLY
                     GTQGFASEYLRIPHPEMLRRAFVLDPLSHLVPRLVLSNQLTVLQNARQRKHHQPALMG
                     ILNVTPDSFSENGINNSIDVRQKIFQQWSEQQVAFIDLGAESTRPGAVSVSAEEEWQR
                     LKPVLGEIKQFFNGNSLAPKISIDTRHSVTAAHALEFGVDMINDVSGLTDPEMLDVIT
                     GSKARYVLMHSLSVPANPQESLPQDLNEVEILKTWFAAKMEWMESKGFPRERLVLDPG
                     IGFGKTRTQNLNLLRGLAEFHDLECPLLVGPSRKSFMNVFCHSPFAERDPETLGISLA
                     ILPDIDILRVHDPVLHHRTLNAFFHARSP"
     gene            4595..5326
                     /locus_tag="VIG33_12205"
     CDS             4595..5326
                     /locus_tag="VIG33_12205"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEY8271644.1"
                     /translation="MRSLQVIAIPTRIFHQGDKILDFLLQHVPVLQEGNILAVTSKII
                     SLGEGAVVSKSSVSKKELIKKEADVYLGELAYDCHLTIKHGLLIPSSGIDESNSENEE
                     YILFPQDPFLSARTLCDSLKSAYKLNKLGLIITDSHTSPLRRGVTGVCLAYAGMEAVT
                     NFIGQPDIFGRQLKMTQINNIDALSAAAVWCMGESNECTPIALIKTESVKFVDSINRQ
                     ELSIGLEEDLYSPLLKAAIKGEPLP"
     gene            complement(5346..5789)
                     /locus_tag="VIG33_12210"
     CDS             complement(5346..5789)
                     /locus_tag="VIG33_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271645.1"
                     /translation="MSALKNQLRLILPKLSKEANANQDSYECTVTSKMLIFKNAPKGI
                     DVSEPATLRLDTSSLAYEFKMYARMKQDVEVARQHDYEETPFRENLISGLLKKDGNIF
                     KDFNDRKNYEFYITPESDKLIIDLTFFHGPSTFEGNDYQFSCEKI"
     gene            6144..7586
                     /locus_tag="VIG33_12215"
     CDS             6144..7586
                     /locus_tag="VIG33_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271646.1"
                     /translation="MKSPKKVILAPLMVFMNIHAALAASVHGDGTNFQSPQGTSESCV
                     AITKLPLGKYSAKDVEKENELCSIDFYNNSVALCPKTWSTSPGTIVRDNSNTGKTSLQ
                     SEASACSQNSPLDALAKFKQTMSRPDTSGTFSLASILYYHFSRVLDATVDVAPAVYRT
                     MDKDAHYARVSSKAHPSPRAKMNVAGWKHLQAAEKNPSTYSPVSDLFTADLQQVYGAL
                     AKSKGERYGVEVNGTRASGWGKGQNLDFQKTPGFMALRSHQPMPQAIQEGVAQAFKDP
                     AMARAFAGVRPSDTQVTLWMKELSEIVIMDYIFSQQDRVGNVDYRWFWVYQDASGKVQ
                     MEKADSKVSLLKKATIKAPQEIAGFHPILVQKTWIGDNDAGGKVNYANFAKATQMVEN
                     IRHISPETYRRLYQLALDFEQQGPNYQVLVNNFGLSADTLKQTVANTKAAASILVKAC
                     EAKTLHFDLVSYKAAYQQQYQEAAVNCRNP"
     gene            8012..11590
                     /gene="smc"
                     /locus_tag="VIG33_12220"
     CDS             8012..11590
                     /gene="smc"
                     /locus_tag="VIG33_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY8271647.1"
                     /translation="MRIKKIELVGFKSFKDRTVIQFDAGITGIVGPNGCGKSNIVDAL
                     MWVMGEMSAKDLRGATMTDVIFAGADGYAPLGMCEVSLTLENDGGAFPAKYIKHSEVM
                     VTRRLHRNGEGEYFVNKEPSRLKDVQEIFMDTGAGSKGFSIIAQGMIGKIITAKPEER
                     RTLIEEAAGITKFKVRKRESQRKLQATDQNLVRLQDIIGELKRQIDSLQRQAQRAERY
                     RNLKNQIEDLDLWLSSIQYHDLRAVADEAQRIFDEAQNMEVEGETNVSTLQAQFETLK
                     LQILEQEKSLEVVQNQYYEKQSSTQKKEMEIQELRFEIEQARRNEQMTGTILQEQQAR
                     HELLLRDHTQLNSQVEALKEESEALTAQYNEKNEIFQNSQSRISEVDEELTSKRRELF
                     AIGQSESSLDARVNSLQAQITDLKDRENNEQMVVTELREKQIEFEDRRRKVFNELEKE
                     RQMQLDLANDFESFEANKKILTEQAATKKIEVEEFKDHLNEVTSRLYGLENLHSNFEG
                     FQEGVKNVMLWQKTKYQEITADGSVVFQPVSEVVEVPNEYEVAMEAALGSRLQMLLSA
                     DSGKAVEAVDHLKEVKSGRSSFMSASESAQKSFASAPTSEPGVQAILKEVVRSNEKYQ
                     NTVAYLLDGVAIVDSIRTALNLRPKYEGWTFVTVDGDTLSADGILTGGSVESADSGVL
                     KRRREIKELTEKKNEWAGKLALAQQALKKVEAQLANVVNDFEGAQKRKIEQEIKVTEL
                     RKDVERAENELQNAQYAVERQERELRKITEQLKEHETRQHDLMINLAEARMKKQDLEG
                     QTEILNTELMSSRMGFDGLQNEVRELQVKSVSRTQEYNGVVRQLEMVNKSLTELDTQL
                     SRMSEESQMYSTQMTDSQMTLEEKKIEFERNIREVEDLKARVSVEKDTYEQMASQIQE
                     MGDELASSQRARNERQSRMNEAQLKLEQARMKEQYLVDQMRERYMLILSDVAEKYQGR
                     EGDPAEAEVQLKDLREKIAKIGEVNLSAIEEYEETAQRYEFLTKQHLDLTEAKEQLRK
                     VIDRINRICSKRFKETFDLVNDRFTRVFPVLFGGGEAKLELHEDVEKGEMGIEIIAKP
                     PGKKMQNVTLMSGGEKALTAVALVFSIFLVKPSPYCLLDEVDAPLDDANVFRFNDLVR
                     EMAKRSQIIVVTHNKHTMEVAGKLYGVTMQERGVSTMVSVSLQDIQ"
     gene            11624..12736
                     /gene="ftsY"
                     /locus_tag="VIG33_12225"
     CDS             11624..12736
                     /gene="ftsY"
                     /locus_tag="VIG33_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEY8271648.1"
                     /translation="MFRELNEDNAILVFAVVGVLCTLIFALLWSFFLRKKSAPPQDAQ
                     EALAKSLENKQGKPLSEQLKEPAKPEVAEVDLRKALRATEENIFGRIKSLFRANEPLA
                     HLEEIEEILYTSDLGPQTVQRLMDALEDMNRKEKADLAKVREILKAEMEGVFSKLPSN
                     SIQFAKEGPTVLMIVGVNGAGKTTSIGKLSSQFASQGKKVLVAAGDTFRAAAGGQLKV
                     WTERAQVEIFCPEGVTDPSAVAFDAVSKAKGQNYEVVIIDTAGRLHTQDNLMEELKKM
                     KRVMARVIPEAPHEVIIVLDANSGQNALIQAKEYNKALTLTGAILTKMDGTAKGGVAV
                     GLANELQIPIKFIGVGERIQDLRRFSSHEFVESIFS"
     gene            12951..14690
                     /locus_tag="VIG33_12230"
     CDS             12951..14690
                     /locus_tag="VIG33_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271649.1"
                     /translation="MTKNVQRETYREVSFFFILLTLTLLVAVQGSAQEGSPRRSASSP
                     SQVMAAAFSSWKVMKPEWGAVEEANYIRYVTMVGEGVANGLCATVSECFRNSRVNPYA
                     FTNPAGLRFFSDCADFPYFLRAYYAWQNQLPFGFVESVSPIQGQNQVVKDIRYSPYGN
                     IVQRRYSVLARKSILGRSKYPNAIDIFNNILPNLISSANYRVSYKGMDTDNLFSDFYP
                     IKITRDSIQPGTVVYDPNGHVVTIYKITRDGRLYYIDAHPDNSLTSGLYNSRFIRSYP
                     GQGAGFKNWRPLRLSGASWDATRGYYGGRIIGAKDRELPLYSTEQYYGTSASPDWKQA
                     QFAFQGKSMDYYDWVRNRLADGPLVENPLDDIKIMTQELCNGVQERVVAVQVALTAGF
                     NQREHPSRLPNNIYGADGDWETYATPARDARLKVAFKELRTYVEEALHHYKSHDGKVD
                     YHGGDLAGDMLKVYKQTASECPITYKNSAGRRVTINLEQARERIYKLSFDPYHCAELR
                     WGAEGKELNSCRDSRTKRLWYDREQWLRYQHERNTDAFTGFTVNEITGPMPGAGVPSG
                     QDLDVIAFLSRNH"
     gene            14736..15347
                     /locus_tag="VIG33_12235"
     CDS             14736..15347
                     /locus_tag="VIG33_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271650.1"
                     /translation="MRVLFFLAFCFVSGRASYSFTDLVQKPKEKPISVSYMNLIKCYP
                     ELKNESLALKVDLNQLKSEIDRKFVTSQSLLRYRQVILKDPNGLQKRLKISAKPAKKS
                     KFNYLLSLEKLDSKGAGTPFELPLPQRLNPSQKDLDQYFLDQDVLEDERSYFDTKLNG
                     LSLSFKRKFDSVFELELSEVSMKRRLVCEDQKDLGIVCTCFQK"
     gene            15529..15831
                     /locus_tag="VIG33_12240"
     CDS             15529..15831
                     /locus_tag="VIG33_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY8271651.1"
                     /translation="MASEKKTHDFLIAGVPYKLKTSHDDATVQELVEFVNHKMDQALG
                     VTKYGSFQNAAVLTALNVAEELILLKRKAQRELEKIEEKALKLSIDLENSKLNRTV"
     gene            15857..16444
                     /locus_tag="VIG33_12245"
     CDS             15857..16444
                     /locus_tag="VIG33_12245"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY8271652.1"
                     /translation="MNVYGPKMQSWGKRQWRDHLKALLQKESPFSSVQIQNQICENLK
                     GYLQNKKGIWAGYLALPQEPDLTSLYRQGEPIDWVYPRMEAEHLAFYLSHHFIKGPFG
                     VMEPPEKSQKIPLEELVGVLVPGLGFNKKGHRLGKGRGFYDRALVDYQGPKIGICFNC
                     QVLEQDLPEEEHDIRVDVLVTEKEIIKCNEFSVMR"
     gene            16456..18021
                     /gene="rny"
                     /locus_tag="VIG33_12250"
     CDS             16456..18021
                     /gene="rny"
                     /locus_tag="VIG33_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEY8271653.1"
                     /translation="MEVVIVAILGLVFGGFVVFVIKKMQDESKKKSARLEADRIVNRA
                     NSEAAKIKKDAETKAKDFESRARKNVETDIHKQKSQMKNKESQLDRRLKEVDDQFKQK
                     MEENERYINQLKDREEKVAISENRIKDLEKKTETHIGELKLKLESVAAMTAEDARRQL
                     ISALEDEAKVEAAKKIAQIEEEAHKEADKKARRIISTALSRFASEYTSERTVSVLALP
                     SDEMKGKIIGREGRNIRTLEAMCGVDLIVDDTPEAVVISGFDPVRRELARRTIEKLME
                     DGRVHPARIEEVVEKQKAEITKSIKEEGDRVVVELGIPNIHPELIKLIGGLKYRSTHA
                     QNVLNHSLEVAYISGLLAGEMGLNSKQARRAGILHEIGRAIEHTAEGSYAIVGAEWAK
                     KYGESEEICHAIRAHQDDEKPTTALAWIVHAANILSASRPGARRPHMDSYIHRLEDLE
                     SIGNSFDGVLKTFALQAGREVRVMVESGKVTDDQSVMLARDIARKIEREMPQSGQVKV
                     TVVRESRSVEHAR"
     gene            18034..19248
                     /gene="tyrS"
                     /locus_tag="VIG33_12255"
     CDS             18034..19248
                     /gene="tyrS"
                     /locus_tag="VIG33_12255"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY8271654.1"
                     /translation="MDPHEQLERIKFGAAEFINDADMLKKLKRSKEKNQPLRIKLGAD
                     PTRPDIHLGHTVVINKLKLFQDLGHHVQFLIGDFTAMIGDPSGKNTTRPMLTREEIEE
                     NARSYKTQIFKILNPEKTEIVFNSHWCEKMNFADVIRLASRYTVARMMERDDFTNRFK
                     SGSPISLHELLYPLTQGYDSVALKSDVEMGGTDQKFNLLVGRDLQSAYGQEPQCVLTM
                     PILEGLDGVNKMSKSLDNYISVVDTPKDMFGKTMKVSDELMYRWYELLTDITAPALTQ
                     LQKDVKEGHKHPRDVKVHLAKFLIKRFHSESAAQQAEEEFNRIFVEKGLPDEVPVFEV
                     FAENQIGLCALMVKVGLATSNSEAGRLISGGGVQRDGEKLSDAKAKWDLKTGDAFVLR
                     AGKKKFVKIIVK"
     gene            19263..19916
                     /locus_tag="VIG33_12260"
     CDS             19263..19916
                     /locus_tag="VIG33_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY8271655.1"
                     /translation="MKVKFIPQNIELEGTPEKSLLQIATENHIEIKSICKGVPSCAEC
                     RVRVVEGENNILPPNKAELSLIGTNWRIDSRRLACQMRCFGDVTVDLTEQLERQENQN
                     KKVRGFRSQKQKESHAVQDTMLLTEKPEEGTMEQEPQRPPQQQKQQQQRQQQGRQQGK
                     QQNQGQRQNQNQNQKQGQKQNPRQNPKQPQKSGSPNNPQTKQSEKAQDRNQKPNPRE"
     gene            19928..20479
                     /locus_tag="VIG33_12265"
     CDS             19928..20479
                     /locus_tag="VIG33_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271656.1"
                     /translation="MQRSRRTNLLVAGLFGFLSSTNLAWGRSMPSMRTLPETHPTFSY
                     DISASSGTENSNTYSELKLNLNWYLNDWLNWRNGIFSRFGSNIQAVNGLDSSLLAAFD
                     TSTEGDQFGVQVFAGPGVRMASADNSAVTAEAGIIFKLGGISLGGGAKYLSYFSTRKD
                     LLGIVLPKDETQYFIVLSGSGSF"
     gene            20526..21344
                     /locus_tag="VIG33_12270"
     CDS             20526..21344
                     /locus_tag="VIG33_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8271657.1"
                     /translation="MAHKITFRERGQGRILILLHGFGGSIHHWESCAEELAKNYRVVV
                     ANLSHLFMSTDRIFFTVQVETIAHFIKTHFPGEKVYIAGLSFGGALTWALSRQHPDLI
                     EKIVLINPVIPNPIPEFSLPELRYFFVMPMNSRAIYVLLSTPIGKAFLKRAAMIFRDE
                     RSEGVGRLDTLKGRRLLFISHLIYNFAWMLRREDWNWWKDKISMTSSPCLMIYDNQDL
                     LFSKEVYRKFTDEIRAQKFLEISGAGHLAIKSQPEVISAAIHEFLTKATEQVAS"
     gene            21505..21918
                     /locus_tag="VIG33_12275"
     CDS             21505..21918
                     /locus_tag="VIG33_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271658.1"
                     /translation="MMAIAGWLMASVLSISLIAEAQIPKTLKCRGDLPIFCNENRSRN
                     PFKLSKGLILVHQQECQAFSVGGTKEKPFIRLSPWAGIQYRTIQANVPHGFQVTLSES
                     SDEDFHILTESKVTKFRKELHCSLQLDLRGKYEVK"
     gene            21905..22186
                     /locus_tag="VIG33_12280"
     CDS             21905..22186
                     /locus_tag="VIG33_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271659.1"
                     /translation="MKLNNFLIFTYCILMAASTFARVKGPLDAPILNADPKLSTDEKL
                     GTGFLFSIIDKVKNNLPEGKLSCAINKYSQVLGIEKYQSTYHMGKTTEG"
     gene            22207..23679
                     /locus_tag="VIG33_12285"
     CDS             22207..23679
                     /locus_tag="VIG33_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271660.1"
                     /translation="MNENPCLAEVASKFAVTLAVKDNESSVSTGSPSSLQEKAGEGRY
                     KNFSPGWAWQHALKFTNGDKNAAMFLIGLCAYEEVPMYSFQVTGKHKVALDRRDIEVL
                     EERLMKAKADLKFFQSEKGYESAKASTLSEVSFIQEAIRQKKTSIENPNQPEEHFLKC
                     PDNGSAFTAPGSLGANVDISGELKEKIRHLQRPQGKNLETLKAKYYHITGSAFMGCQL
                     KKCGMPNMVTKLWESQAGRMYRSIRLCGQVKTNLKNIQVISKKIGMDINEPKFRETAK
                     LYLQAEAKKLIVEGQDHVKMRDALYAQGLRGPELTKRLPSYRCNDATLRETNPQVAYL
                     CSATFLQREADSNSEAVAREVGETDRAIDRLLQEYDASIMFRKWYIGGDNIPCTGIKA
                     AGPFQLDGNNPNCEMYGWSAERCSTALAKLGTWDVDFDWTVAQHKVGAEFGAEQCREQ
                     DPNEKLDKTFCKILDENNKKELYGEGKAPAPPSEEIQGAR"
     gene            23780..24331
                     /locus_tag="VIG33_12290"
     CDS             23780..24331
                     /locus_tag="VIG33_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271661.1"
                     /translation="MKSMIVFVSGLFLSFNVMAAEKTLSTAIPATAFASSTMTNDALL
                     PSTLAFNFVALSQSKTNIYFDVGGLSERVAPALSFRSYSNKEVRKKLNNDKLTVDRSL
                     ATLGASIAVLKGDAKALILNPYLYFGTEKDAVNTENVNGVGVRLLGQANVYKGLGFQA
                     GIDANNMEGEFKNDLYVGLAFAL"
     gene            complement(24387..24764)
                     /locus_tag="VIG33_12295"
     CDS             complement(24387..24764)
                     /locus_tag="VIG33_12295"
                     /inference="COORDINATES: protein motif:HMM:NF020360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HI0074 family nucleotidyltransferase
                     substrate-binding subunit"
                     /protein_id="HEY8271662.1"
                     /translation="MDELTNFEKTLKQLKSFVALPIQNDRDKAGIIQAFEYTFEQCWK
                     SIQKKAGHEGVQIGSPKKAFMFAFQNNWIAKESENFWLQMIEDRNLTSHTYKEDIATE
                     VLQRIVRDYIPCFNDLIFNLRKE"
     gene            complement(24757..25068)
                     /locus_tag="VIG33_12300"
     CDS             complement(24757..25068)
                     /locus_tag="VIG33_12300"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY8271663.1"
                     /translation="MPAFTKNFTQLPNSIKDLIQYAIEKVHPEQVVLFGSRARGDHRE
                     KSDFDIAMKGSIAPEAWTQLQVELDEKALSLYSVDLVLYQDLGEDYRKNIQVEGKVIY
                     G"
     gene            complement(25070..25396)
                     /locus_tag="VIG33_12305"
     CDS             complement(25070..25396)
                     /locus_tag="VIG33_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY8271664.1"
                     /translation="MITISESAAKKLALLKVEEGQSDSAFLRVEVKKGGCSGLSYKMN
                     FDTVSREGDKVFETNGQKVVVDPQSILYVLGMTLEFSGGLNGKGFVFNNPNASKNCGC
                     GSSFNV"
     gene            complement(25502..26662)
                     /locus_tag="VIG33_12310"
     CDS             complement(25502..26662)
                     /locus_tag="VIG33_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEY8271665.1"
                     /translation="MTSPIYLDYNATTPVDPQVFSIMEPYFKTHFGNPSSFAHQWGWA
                     AQKGVEKARTQVAALLDAKSFEITFTSGATESNNWVIFGLVNKFQSENPGDPLHILAS
                     DVEHSSIRNSLMEVQRRGVEVDFVKTNKYGQLDIEAVTKAIKPHTKLMSFIWVNNEIG
                     SINPMQDLVKLAKEKKIYIHSDATQALGKIPVNMHEIPVDFLSASAHKFYGPKGAGIL
                     YMRSNQPKVTLPPLIFGGGQESGHRSGTHNVPAIVGLGAAAELCQKIISEEMARTTEL
                     RNYLWTGIQKNIPGARLNGHPTERSPINLNVTFPGKNIEQLLPKLGRLGFSQGSACHT
                     GSLEASPVLKAIGLSSQEAQGTIRLSLGRWTTKEELDIAVEILTQAMGADKV"
     gene            26727..29033
                     /locus_tag="VIG33_12315"
     CDS             26727..29033
                     /locus_tag="VIG33_12315"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEY8271666.1"
                     /translation="MAKKKLQNLVFAEHFTAGVKEAETLFPWGRRDCLIKDRRGGIFF
                     EMKNVEAPQGWSQLALDIAASKYFRKKTGVFKGEHSVQQMVHRVVAAIAKAGTRQGGI
                     FANKKQTDIFIKELKYILYSQRAAFNSPVWFNVGLDSYGIVSKSEHFAWDEKSKAFKK
                     INDAYERPQCSACFIQSVDDSLESIFNLAKSEAKLFKYGSGSGTNFSRLRSKYEPLLS
                     GGGTSSGLISFLEVLDRGAGAIKSGGTTRRAAKMVCVDLDHPEILDFIDWKMKEERKA
                     HVLIAAGYDADFEGEAYRTVGGQNANNSVRVPHVFMESLLEHKDWELKARVSGMARNK
                     VLKTLPAKEVWDHIVHSTWVCADPGVQYHDTINEWHTCPQAGGIRASNPCSEYMFLDD
                     SACNLASLNLTKFMTPEGDFDVVSFMHVARIMFVAQEILVDYSSYPTEKIAENSHMYR
                     PLGLGFANLGSFLMRKGLPYHSDQARAWAGAIASLMHGMAYWTSSQMAETRGPFKGYA
                     KNKKSMLKVIGRHAEAVSKIPWTLLPDYFKATCEELWQDVVEMGTQYGFRNAQATVIA
                     PTGTIGLLMDCDTTGIEPDFSLIKYKKLVGGGEVEIVNQSVPFVLHLLKYSEKEIESI
                     QAYIQEHRTIEGAPYLKAEHLPIFDCASAQQAGQRTLPPESHILMMAAVQPFISGAIS
                     KTVNMPGSASEKEISEIYLQSWKLGLKAVAIYRDGSKASQPLNIKKEEAKAGLPVGTM
                     KCPECGVDTVLTSGCYRCPNCGSTVGCA"
     gene            complement(29036..29281)
                     /locus_tag="VIG33_12320"
     CDS             complement(29036..29281)
                     /locus_tag="VIG33_12320"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEY8271667.1"
                     /translation="MNNHKLGNDYIHRAHGRLKAIDTLLNEKLFADVVRESQKACELA
                     LKGAIYNAGHSVPRIPEVSHTLEAMRQLKEVLKIIPR"
     gene            complement(29329..30438)
                     /locus_tag="VIG33_12325"
     CDS             complement(29329..30438)
                     /locus_tag="VIG33_12325"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEY8271668.1"
                     /translation="MQVAIISIGTELTIGQIINKNSAWISKNLKDLGVPTRCHLVVPD
                     DRELILDALRFCAERCDTLFVTGGLGPTTDDFTRELISEWSGLALEFHEASWEHVNQR
                     LKSRGVNVREAQRQQCFFPKGARILPNSEGTANAFQLRVFNKDVIVLPGPPNEIAAIW
                     KSSLDSFLREKTKDLDKSITYSWETLGLGESDVAHITEDCLRGIEIEKGYRAHLPYVE
                     VKVTFLESRTKQLMPAIQRLDEALHNVTVARNGEDVPSLIAKKLTHVKELQVVDSLSG
                     QAVLNRLSPVSATGATTWSFSNHNNFSADPQVLKMILRPWGTTGAIAEISYRGASFKT
                     ILEAPSTIPKITERKALYFAERAMIFWLQKLEDLI"
     gene            30557..31780
                     /locus_tag="VIG33_12330"
     CDS             30557..31780
                     /locus_tag="VIG33_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8271669.1"
                     /translation="MSELKVDAHWKEFDLYNPTPEHAMLRETIANFTKEEIEPQAHEY
                     DRKERFNLDLFRKVGELGLLGITVPEAFGGAGMDATAAVLVHEEMSASDPGFCLAYLA
                     HSMLCVNNIAMNASDAQRRKYLPQLCSGEWIGSMSMSEPAVGTDVMGMQTAAVKKGDK
                     YILNGRKMWITNGTIDENKTPCDLTLVYAKTGEKNGRALISSFLVEKSFKGFQVGQKI
                     QDKLGMRGSNTAELVFQDCEVPVENLIGAEGDSMMHMMKNLELERLTLAAMSLGIARR
                     SVEIMNRYATEREAFGKSLNFFGQVQRYIADSYAEYKAARTYVYETARRMDLTKEGNR
                     MDSDGVKLVATTMAKNVADRAIQVLGGYGYVGEYVVERLWRDAKLLEIGGGTIEAHQK
                     NITRDLAKNPEALYK"
     gene            31790..32500
                     /locus_tag="VIG33_12335"
     CDS             31790..32500
                     /locus_tag="VIG33_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY8271670.1"
                     /translation="MFPHPERISVDTHLSVHYQLILEHIGPLLTAERRQKIDRVVAGR
                     NFETAVVLEGIYDRGNSSAVMRTAEGLGFANFHVIETQEKFKESNRVTQGADKWVETQ
                     KWKQSKECIQSLKDQGYRICVTTLEASKPLSEVDFSGKTALVLGNEKNGASQEMIAAA
                     DERIIIPMPGFVQSFNISVAGALCLYHIMQDRIRRTGSNADLTEKEQDILKALYYMRT
                     QDSAQDYLKEMFSRGTLA"
     gene            32521..32925
                     /locus_tag="VIG33_12340"
     CDS             32521..32925
                     /locus_tag="VIG33_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271671.1"
                     /translation="MRGGIKKIAVAVLFTLGGSLNSFAREKAFELKMELTINGKHISS
                     PRIIAKEGETAMFMQEDQTQKTFIEVVATEDKIVRGKHSVLMKFVVGSIDPDGQKKVI
                     YKPQVLTLVDHKTDLSVVEDIGLSVTASRVSL"
     gene            complement(32922..34574)
                     /locus_tag="VIG33_12345"
     CDS             complement(32922..34574)
                     /locus_tag="VIG33_12345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8271672.1"
                     /translation="MTHNQIHVTLKDMGSSPTDFIFTQKPSGFNTHSPDIGKKGYVEI
                     LSEIRQQELFVVHRLDKETSGAMLFATNKQSAAELSVLFEKHLIQKKYLFLTDKKINR
                     DQFEYESRIEKDKGQFVNILNMTPNAKTSFTKLKSLGAYDLWLAEPTTGKPHQIRLQA
                     QANGISILGCREHGGSPFYRLCLHAESLNFEYKSQKFSFQADAPVWVKELEQDLNIDQ
                     EELMILEACQKRQWLLNFQAKPNECFRLSHQDIDSYRIDLFGDYLWFYWYKDHDPAEK
                     DLQRFERVAKRLGKKYFVRKMLNRGEDPNAQILWPSKESLRWIACENGVKYDCRNDTG
                     LSPGLFLDQRENRRWVREHSKDKAVLNLFSYTGGFSVNAALGGAKEVVTVDVSSNFIE
                     WSKKNFELNSLPLPHSDTLAEKPKLDFKSIMSNEVPKPLYEFWVQDSILFLKGTARRK
                     RKFDMIICDPPSFGRSKNGTFSISKNYEELLVNCLYCLGKGGLLLFCTNYEKWTQADL
                     IKNIQKLKSQFHFTLLASPGSCLDFELPDEEPLMKSIIIRKT"
     gene            34621..35214
                     /locus_tag="VIG33_12350"
     CDS             34621..35214
                     /locus_tag="VIG33_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY8271673.1"
                     /translation="MKAFIKLMALFLTLSVLAGCGVQSIPQAKNQVEASLAEVTNQYK
                     RRADLVPNLVNVVKGYAKHEEQTLTSVIEARAKATQVTLDPTKVTPEKLREYQAAQSG
                     MSQALGRLMVVSEQYPNLKADSQFRDLQAQLEGTENRITVARQRYIQAVQEFNNYVTV
                     PPTNWTNSLLYHYDKMPQWDVAEDEKGKVEKPPQVNF"
     gene            35219..35971
                     /locus_tag="VIG33_12355"
     CDS             35219..35971
                     /locus_tag="VIG33_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY8271674.1"
                     /translation="MTYRLFSFLLLFCTGICSLARADFEVPRLTGPVMDEGGVIRPQD
                     RNELEQVIRDYNSQGKAQIQILVIDSLQGLEIEEATIKITDKWKLGTEKKDNGILFLI
                     APNERKLRIEVGQGLEGTLPDVIAKRIISDTVLPLFKARNLSAGIVVGTYQIIKYIDQ
                     EYADQHLSEPAPESPKSIPGWVILVILLLLLFIGRFLPASSFRGGGFRGGGWGGGSFG
                     GGGGGGWSGGGGGGGWSGGGGGFSGGGASGSW"
     gene            35975..36652
                     /locus_tag="VIG33_12360"
     CDS             35975..36652
                     /locus_tag="VIG33_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271675.1"
                     /translation="MKQRWVTKYLTEKQIAEISQAVHQAEGGTSGDIVPMIVRRSSAV
                     RHVPVILGLSFALIFFGIEILGVNGWYISDFFPFLMGHWVYQIGFLALLMVLAWGLSH
                     LHPVQRLLTSDEDEKEQVWRRAQLEFFLHRLHHTHKKTTVLIFVSVMERRAVILADEA
                     VAKVLPANTWEEVLKPLTGFLHKGEWSAGFQDSIRRSGALLKAHFPEPVGGTPANEIS
                     NNLIIKA"
     gene            36753..36829
                     /locus_tag="VIG33_12365"
     tRNA            36753..36829
                     /locus_tag="VIG33_12365"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36787..36789,aa:Arg,seq:tcg)
     gene            37201..38166
                     /locus_tag="VIG33_12370"
     CDS             37201..38166
                     /locus_tag="VIG33_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271676.1"
                     /translation="MLKILTVLCLSGALSCFASANMCAQLFSPLSFTPSHFASSTFGL
                     VHAERPGQARYSQWSADTANEVLLAIAAGKPAKELFEIGVRARQEFARKFENVDKSEE
                     DFGASRVTRSRNIVGEDMIGLSSLTLDRRRGQALPAHFDAWPNPWWANIARTESRTQL
                     ADSILIHLVETQRLSTTDYNLLIWDRFYQTSTGEVLKSSSTKIQRAFPEQQDSPYFVS
                     TLNAPKPEIELLMTDVYRRIQLALKAPTKKEARENLSHAMYGFFNAMPYYRGSAAIGR
                     IVFTVLFSSIEERPMIIDPECDVKALVQSQFEFLTNIQSIVRTGK"
     gene            38889..40199
                     /locus_tag="VIG33_12375"
     CDS             38889..40199
                     /locus_tag="VIG33_12375"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY8271677.1"
                     /translation="MKRFLIFSLVFSLVLSFGTALYAQDLLNSIQAPEEMTLLSTSKT
                     ETLNLGISLSSQSNSKILKKVWKTASEKACSAEMQKSFSEEARLKLERQLKSEDIIDD
                     LAAVLNPFLFSLGMSHTEFMTATSESYYFFKSYEALLHPEQRPAPHILNPGVQVGKDK
                     DGYYAREVLTGFPADLAGLKKKDRLVSLDGQAFSGTWGSSPKVALVTVQHLGQLSNLP
                     IEIKSLDWNQSFQDATKSSVQIFTGKNGKKIGYIHLWTGVHPQSARFLKDSVLNLKRQ
                     KISSIILDLRGGYGGAWWEHLEAFYPDSSTYMQLEVMATDGETEILKSPFKKNKNYFS
                     GPMAVLINEGVRSGKEALAHQFKKTKRAYLIGEKTPGYFSTGQYFYVNEPVDYALYLC
                     VYQLKLDGKILEGIGIEPDEDVIYKPSGPFGDSQLEAAVDYLSK"
     gene            40504..41832
                     /locus_tag="VIG33_12380"
     CDS             40504..41832
                     /locus_tag="VIG33_12380"
                     /inference="COORDINATES: protein motif:HMM:NF025029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEY8271678.1"
                     /translation="MKVLYKKSFISLILLMPVFPILTLAHSGISQLKEGDVLELQKRG
                     RNARVSPNFSKHNKSNLAFGSDSRLGPGTLVRYTGKYEGTKQGNFGIQVEILPQEGKP
                     FDERHRGKLVWIYYRNHERSIVEKPGEVATGPINPADITEEKNLIRPDESPEDFSEKN
                     SVAQTEDNEVPEVIQEAQNTLDKIGKINSGLSCTNSTCREEDNDSARPLQATDALLNY
                     ELGEALTSSWAQEEVLKAGIPSTPLLRALHFYNANKDLRNLQRNYLVVADLTQASNKK
                     RMYKIDLRSGKVSKYTTSHGRGYGNAYYCANTFGNRRDSNLSSGGGYVTGPSRSFRGS
                     KALNLHGVEVGKNDRAFSRGVLLHRASYVNDHQAGRTWGCLSIADKYSKEFISSLEGH
                     GSNSGFIGQGSAIYVYPDRDDILNKNSYWDPSCRANLNAQGDRYKPRWIP"
BASE COUNT        11767 a         8792 c        10063 g        11388 t
ORIGIN      
        1 ctcaaatggc ataatatgca accttgtcgc gtattatgcc atttgcttta aagagggatc
       61 cgtggcgatc cgtcttcgca aatctatatt cgcaaatcgc aaaaaaactt ggcctatttt
      121 gtcccattgg ctaatccctc aacagtggac ccttttggca agcagttatc cgtaaataaa
      181 catggcatgt ttgtcgcatg aggaatcaaa gaagatctcc aaataattgt ggttcatatt
      241 caaataggat tagaaaaatg aagaaaatta attttttaat tatgtcactg gtaatagtcg
      301 caggcactgg agtttcctct aaaggatttg cttatggcgg atcgcatttg gtttggattt
      361 gtagtggtca ataccaaata ttttcagatc atgtagaaca gaaaataatt actggttcag
      421 agctgaaaaa gattgtgtct gaacatttag ataaaaaaat acaaagtcct agctatgtca
      481 tctgccccag atcaacgggt ggcggttaca attttctctt aactcttttt ggacaagaag
      541 aaaatcatac cttagtggac tcctatattg ttagtggtga agatttaaaa aagtttatgg
      601 agtgaggaaa gtcttttctg cttatctcct agacagacac tttttaagcc ggggtttttg
      661 cattgcctag gtcccctttc aggggttctg tctggcttat aattgcaggt tatttagggt
      721 tgcaagcaat tgattttaat agaaagccat tctcgtaaaa taggagtgac ccttgaatta
      781 caaattatat cttaaagttg tttccgttac ctttttacta ggattcgtta atttctcttg
      841 ggctgtggac gttcagaatg gctcttcctc ttttttatcc gatagcttaa ccaaaatgat
      901 tcaacttttt gccaatgata aaacttatga cgaactgaaa ctacgattgt ggaatgaata
      961 tctgtcctat agtgagcgtt atgcggagaa gttcgctgag gtggccaagg aaaatcaaac
     1021 ttcattgaag agctatatgt gcgcctccgg aaacccctcc gaaagtgcta agaaaattca
     1081 aagtttccaa cgcacaactg tgaatgattt caatctcaag aacgatcgag atcagataga
     1141 aatagctaaa ctgcagcagc ttcaagcgac ctataaagaa atttgtgagg cttctgggga
     1201 cggcttggct ttaaataata aagcttctat ggtgattgca gaaactactt tttattcgat
     1261 ctatatttcc actcgtgacc aattgatcgc atttttaaat ttaaaaaact aaatttaaaa
     1321 aattagtctc aactaaaata tctgagtcaa tcaatgctct ccaataactt gagcaattta
     1381 ttttgcaggt gagaactgcg gggttgctta aaataaataa gccagacgta atccagaagc
     1441 cgacggtttg aaggcttgag gatatgcatt aaattgtctt tgttgccaag ttcgaagaca
     1501 tatttaggaa ccacgcccac gcccagtccg cgtttaacaa attccaccac catatcggcg
     1561 gtggctgcat aacatcgcag attcaccttg gcgagatttt ggtgataatg gcatttccac
     1621 cagcgacgga aaaggacgtg gttggggtaa taatcgatga ccggagccat catgattttt
     1681 tcgcgttcgg gattttccga ccattgatcc ttacgggcga tcagcaccaa ctcttcttga
     1741 tagagctttc gactagtgat ggaactcttt cggccttcat agggaaaaat actgatgcat
     1801 agatccagtt tttgctgttc cagggccaaa agtaaacggg acggagagtg aaaagagaat
     1861 tttatttgca cgcgaggttc ggtctccaga aatttctgca gaaccggcat cattttgctt
     1921 ttcgtaaatt caagataaga gccaatacgc agagtaccgc tggattctgt ttgaggattg
     1981 gtgaactgcg acacggaatt ctcaatctcc agcagggatc tggcgatatt gtcatggaat
     2041 tgctgcgcgg tttgcgtgag caccagtttg gtccccacgc gatgaaaaag agcataaccg
     2101 agttgactct ctaatcccgt caaagattga ctgatcgcgg atcggctcag acctaaatct
     2161 tcagaggcgc ctttgtaacc cccgtgctga acaacagagc agaggacata taatttgttg
     2221 agatcgatac cgtttagttt cattaaacgg atcttttaga tcatttatat atttgaatca
     2281 acttcaatat gctagcgtcc gttgcaaatc taccaggagg tcttgtatga acgaaatcac
     2341 gtctttgatt caaactttcc atttgctaaa acatcctttt tatcaaaaat ggatggcggg
     2401 tgaattgact caagagaatc tgcaaaatta cactcttcaa tactatcccc atgtgaaagc
     2461 ctttccacga tttgtcagtg cgatccatag tcactgtgaa gatctccata gccgaaaaat
     2521 tttacttgag aatttactcg acgaagaggg ttatccaaat gcccttcctc atcctcagtt
     2581 atggaaagat tttggccagg gtctgggtct tgaagactct gactttgagc aaaaacccat
     2641 cggaccgaaa gcccaagaga tggtggagtg ctttgatagg ctcactaaaa aatcctatgc
     2701 ctctggactt ggagcccttt acgcttatga atcgcaaatt ccagaaattg cggcggcaaa
     2761 aattcagggt cttaaagagc gatatcaaat tgaggatccc gcaaccttgg cattttttac
     2821 ggtgcatgaa acagcggata aatatcattc agaatcttgc gcgaaattga ttgaatctct
     2881 ttcagagcaa gaaaaagcgg aagcctgtgc cagcactagg gaagcttgtt taagtctgtg
     2941 ggactttctg actgaggtga atgtatgatt ttggaaatcc accggttgat gttgaaagtt
     3001 cgtttgggtt gcacggccgg ggagcgtttg catcctcaag atgtggccgt gagcgtttcg
     3061 atgaccttcg ctcatggtgt ccaggccttg gaaagtgatc agctttctga aactctttgc
     3121 tatcaagaga tctgccagat gctcaaaacc gtggccatgg ctaaagagta taataccgtg
     3181 gagcatttgg cttttcagat gagtggagtt ttaaagcctt tgttgagaag tctcgctagc
     3241 gcggaactct cggtgcacaa aatagctccg cccatcgaag atcttacagg cggtgtgacc
     3301 gttcggctga cagtgacgtg atggagagcg tgatgagagc ccgggttttt ctgtccctcg
     3361 gagcgaatct gggagatcgt aaacagacac ttcataaagc ggtgcaactt ttgcctcttg
     3421 aggataagaa gctatcacct ctttttgaaa ctcctgcttt gttgccctca aatgccgatt
     3481 cctggaatat tccatttttg aacttagtag tggagggaac cacagaagct cccgcagagc
     3541 agcttttgga aactctcaaa ggcatagaaa gggacttggg aagaaaaccc gcggcgcgct
     3601 gggctccacg gcttatcgat attgatatct tattgtacgg aactcaaggt tttgcgtccg
     3661 agtatttgcg catacctcat cccgaaatgc ttcgacgggc ttttgttttg gatcctcttt
     3721 ctcatttggt tcctcgctta gttctgagca atcagctcac ggttttgcaa aacgcccggc
     3781 aaaggaagca tcatcaaccg gccttgatgg gtatactcaa tgtgacccct gattcttttt
     3841 ctgaaaacgg aataaataat tccattgatg tccggcaaaa gatttttcaa cagtggtcag
     3901 agcagcaagt ggcatttatt gatttaggtg cggaatccac tcggccgggc gcggtttccg
     3961 tgtctgccga agaagaatgg caaagactga aacctgtatt aggcgaaata aaacaattct
     4021 ttaatgggaa ttctttggct ccgaaaatca gcattgatac ccgtcatagt gtgacggcgg
     4081 cgcacgcttt ggagttcggc gttgacatga tcaatgatgt gagtggtctg acggacccgg
     4141 aaatgctgga tgtgataacc ggctcaaagg ctcgctatgt tctcatgcat agtttatctg
     4201 tgcccgcgaa tccacaggag agcttaccgc aggacttaaa tgaggtggag attctcaaaa
     4261 cttggtttgc tgcaaagatg gaatggatgg agtccaaggg ttttccccgg gaacgcctgg
     4321 ttctagatcc gggcattggt tttggcaaaa ccagaactca aaatttgaat ctcctcagag
     4381 gtcttgccga gtttcacgat ctggagtgtc cgctgttagt agggccctca cgaaagtctt
     4441 ttatgaatgt cttttgtcac agcccctttg cggagcgtga tcctgaaacc ctgggcatca
     4501 gcttagcgat cctgccagac attgatattt tacgagtgca tgatcccgtc cttcaccatc
     4561 gaactttgaa cgcatttttt cacgcgaggt ccccatgaga tcccttcaag tcatagcgat
     4621 tcccacccgt attttccacc agggagataa gattttggat tttttactcc agcatgtacc
     4681 ggttctgcaa gagggcaaca tcctcgccgt cacttcaaaa attattagcc ttggggaagg
     4741 ggctgtggtc agtaagtcct cagtttcaaa aaaggaactg ataaaaaagg aagcagatgt
     4801 ttatctggga gagctggcct atgattgcca tttaaccatt aagcacggat tgcttattcc
     4861 ttcctcagga attgatgaat ccaactcgga aaacgaagaa tatattttat ttccccagga
     4921 tcctttttta agtgcgcgga ctttgtgtga ttctttaaag tccgcttata agcttaataa
     4981 gcttgggctt attattacgg attcgcacac cagtccttta cgcagaggag tgacgggggt
     5041 ttgcttagcc tatgccggaa tggaagcggt tacgaatttt atcggtcagc ccgatatctt
     5101 tggacgtcaa ttaaagatga cgcaaattaa taatatcgat gccctcagtg cggcggcggt
     5161 ctggtgcatg ggtgaatcca atgagtgcac gccgatcgct ctcattaaaa ctgaatctgt
     5221 aaaattcgta gactccatca atcgccagga actttctatc ggcctggaag aggatctgta
     5281 ttctccgctg ctcaaggcgg cgatcaaggg cgaaccgctt ccataactcg atttctatct
     5341 ttcttttata ttttttcaca actgaactga taatcattgc cttcaaaggt gctaggtcca
     5401 tgaaagaatg tcaggtcgat gatgagctta tcagattcag gagttatata gaattcataa
     5461 ttcttgcgat cattaaagtc tttgaaaata ttaccatctt tttttaaaag acctgaaatt
     5521 agattctctc gaaaaggcgt ctcctcatag tcgtgttgtc ttgcaacttc aacgtcttgt
     5581 ttcatacggg catacatttt aaattcataa gccaatgaac ttgtatccaa tcttaacgtc
     5641 gcaggctctg agacatcgat tccttttgga gcatttttaa aaatcagcat tttactggta
     5701 acggtgcatt catagctatc ttgattggca tttgcttctt tagaaagctt aggcaatatc
     5761 aaccttagct ggtttttcag cgcagacatc tctttcactc ctttgttttg ggtttatcgc
     5821 aatagaatca aagcattggc tggaatgaga tgtctgtctt ttcctaccta actggaccaa
     5881 caaacttaat gccaatacca gtaacccaat aactcccact atttggataa aatctgccgc
     5941 cgatgtccca aagttctcta cattgtcgaa cttttgtaca gggaccagga gggtcttaag
     6001 tgagatactt aaacactttc ttttatcgtc gacgcagtaa accgcatttg cagatggcct
     6061 gattttagca aaccttaact tcgataaata tttttttgaa aaactgggaa atccatcccg
     6121 cagaaatttc gaggtatcga tttatgaaat cccctaaaaa agtgatctta gctcctctta
     6181 tggtgttcat gaatatacac gcggctctgg ctgcttctgt ccatggggac ggaacaaact
     6241 ttcagtctcc acaggggact tctgaatcct gtgtggcgat tactaaattg cctttgggga
     6301 agtactccgc caaagatgtt gaaaaagaaa atgaactttg ctcgattgat ttttacaata
     6361 attctgtggc tttatgtccg aagacttgga gtaccagccc aggcaccatt gttcgtgata
     6421 attcaaacac cggaaaaacc tctcttcaat cagaagcttc tgcttgcagt caaaactctc
     6481 cgttggatgc gcttgctaag tttaagcaga cgatgagtcg tcctgatacc agcggtactt
     6541 tttcattagc gtctatttta tattatcatt tttcgcgggt tctggacgcc accgtcgacg
     6601 ttgctccagc agtctacaga acaatggata aagacgccca ttacgcgcga gtctcttcaa
     6661 aagctcatcc ttctcctagg gcaaaaatga atgtagctgg ttggaagcat ttgcaagcgg
     6721 ctgaaaaaaa tccttcgact tattcaccgg tgagcgatct cttcactgct gatttgcagc
     6781 aagtctatgg ggctttggca aaaagcaaag gagaaagata cggtgttgag gtaaatggca
     6841 ctcgggccag tggttggggg aaaggacaaa atttggattt tcaaaaaact ccaggcttta
     6901 tggccttgag atctcatcag ccgatgccac aagccattca agaaggcgtg gctcaggcat
     6961 ttaaagatcc tgcaatggca agggcttttg ccggagttcg tccttcagac acccaagtga
     7021 ctttgtggat gaaagagctg agtgaaattg tgatcatgga ttatatcttt agccagcaag
     7081 accgcgtcgg aaatgtggat tatcgttggt tctgggttta tcaagacgcc tctggcaagg
     7141 ttcagatgga gaaagcggat tctaaagtaa gtctattaaa gaaggccacc attaaagctc
     7201 ctcaggaaat cgctggtttt catcctattc tggtccaaaa aacttggatt ggtgataacg
     7261 atgccggcgg aaaggtcaac tacgccaatt tcgctaaggc gactcaaatg gtcgaaaata
     7321 tccgtcatat aagccctgag acttatcgtc gcctttatca gttggctttg gactttgaac
     7381 agcaaggccc gaactatcaa gttttagtaa ataactttgg tttgagcgcg gacactctaa
     7441 agcaaacagt ggctaatacc aaggcggcgg cttcgattct ggtgaaagct tgtgaagcaa
     7501 aaacccttca ctttgattta gtaagttata aagctgctta tcagcagcaa tatcaggaag
     7561 ccgcagtgaa ctgccgtaat ccgtaatccg caatccatag gtctattaga cccagagaag
     7621 gtcgtgtcgg ttctgttgac ttatcggagt taacagaacc ctcaagccga gtagcgtatt
     7681 atgcaattca gttgcgtaat acgcgattga gtttgaacgg tcgtgcaagc tcctgccctt
     7741 gtcgcgagcc ttacttttta aaaaattaaa atcatgaatt cagaaaatgt cttatgaagc
     7801 tgcagttgta aacgcagcgc gctaatcgtt tccaaactct gcccaagttt tcagcttgaa
     7861 gcctgtcttt agtaggcttt gattgacaca atctcaatcc ctggcgagag ttaaacttca
     7921 attacctggt ctatcaacta gtccagtcgc tgggctttcg gctggttcgg actttataag
     7981 ctacttttag aaggaattaa gaggggttaa tttgagaatt aaaaagatcg aactcgtagg
     8041 gtttaagtcg ttcaaagacc gcaccgtcat tcagtttgat gcggggatca cgggaatcgt
     8101 gggacccaat ggttgtggaa aatcaaatat cgtggatgcc ctgatgtggg ttatgggaga
     8161 gatgtcagcg aaggaccttc gtggcgccac catgacggac gtgatctttg ccggagccga
     8221 tggctatgcg ccgctcggta tgtgtgaagt ctctttgact ctcgagaatg atggcggggc
     8281 ctttcccgca aaatatatca aacattctga agtcatggtg acccgacgcc tgcatcgcaa
     8341 cggcgaaggg gaatacttcg ttaataaaga accttcccgc ttgaaagacg tacaagaaat
     8401 tttcatggac actggagctg gttccaaggg tttctccatt atcgctcaag ggatgatcgg
     8461 taaaattatc acggcaaaac ctgaagagcg tcgaactttg atcgaagaag ccgcgggtat
     8521 caccaagttc aaggttcgta agcgggagtc gcaaagaaaa ttgcaagcca ccgatcaaaa
     8581 tttggtgcgc ttgcaagata tcatcggcga gttaaaacgc cagatcgact ctttgcaaag
     8641 acaagcgcaa cgggcggaac gctatcgcaa tcttaagaat caaatcgaag atctcgatct
     8701 ctggctcagt tctatccaat atcatgatct gcgcgcggtg gcggatgaag ctcagcgcat
     8761 ttttgatgaa gcgcaaaaca tggaagtgga aggggaaacc aatgtttcca ctttgcaggc
     8821 tcaatttgaa actctgaagt tgcaaatcct ggagcaagaa aaatctctgg aagtagtgca
     8881 aaatcagtac tatgaaaagc aatcttccac gcaaaagaaa gagatggaaa tccaagagct
     8941 gcgctttgaa atcgagcagg ctcgccggaa tgagcaaatg accggaacga tcttgcaaga
     9001 acagcaagcg cgccacgaac ttttgctcag agatcatact cagttgaatt ctcaagtgga
     9061 agctttgaaa gaagagtccg aggctctgac cgctcaatac aatgagaaaa acgaaatatt
     9121 ccaaaactct caatcccgca tcagcgaagt ggatgaagag ttgaccagca aacgtcgtga
     9181 gttgttcgcg atcgggcaaa gcgagtcttc gttggatgct cgtgtgaatt ctttgcaagc
     9241 ccagatcacg gatttgaaag atcgtgaaaa caacgagcaa atggtggtca cagaacttcg
     9301 tgaaaaacaa atagaattcg aagaccgtcg tcgcaaggtt ttcaatgagt tggaaaaaga
     9361 acgtcaaatg caactggatt tggcgaacga ctttgaatcc ttcgaggcca ataagaaaat
     9421 tcttactgaa caagcggcga ccaaaaaaat cgaagtcgaa gagtttaagg atcatttgaa
     9481 cgaagtcact tctcggcttt acggtttgga aaacttgcac tctaatttcg aaggcttcca
     9541 agagggcgtg aaaaacgtca tgctttggca gaaaaccaaa tatcaagaaa tcaccgctga
     9601 tggttctgtt gttttccaac cggtttcgga agtggtggaa gtacctaacg aatacgaagt
     9661 ggcgatggaa gcggccttgg gttctcgtct gcaaatgttg ctttcggctg atagcggtaa
     9721 agcggttgaa gccgttgatc acttgaaaga agtgaaatcg gggcgctcca gttttatgtc
     9781 cgcttctgaa tcagcgcaaa agtcttttgc ctcagctccg acttctgagc caggcgttca
     9841 agccatcctt aaagaggtgg tccgctcgaa tgaaaaatac caaaacaccg tggcctattt
     9901 gttggatggg gtcgccattg tagactctat ccgcacggcg ctgaacctgc gtccgaaata
     9961 cgaaggctgg accttcgtga cggtcgatgg agacacgctt tcagccgatg gaatattaac
    10021 cggtggctcg gtcgaatccg cggattcggg cgttttgaaa cgccgtcgtg aaatcaaaga
    10081 gttgaccgaa aagaaaaatg aatgggcggg gaaactcgcc ctggcgcaac aagctttgaa
    10141 aaaagtggaa gcgcaattgg ccaatgtagt gaatgacttt gaaggggctc aaaaaagaaa
    10201 aattgagcaa gaaatcaagg tgactgaact gcgcaaggac gttgagcgcg ctgaaaatga
    10261 actgcaaaac gctcaatacg cagtggaacg ccaagagcgc gagctgcgta aaatcaccga
    10321 acaactgaag gaacacgaga cacgtcagca tgatctgatg atcaatctgg cggaagcgcg
    10381 catgaagaag caagaccttg agggacaaac agagattctg aatacagaat taatgtcaag
    10441 ccgcatgggt tttgatggac ttcaaaacga ggtgcgtgag cttcaagtga aatctgtttc
    10501 cagaacacaa gagtataacg gtgtcgttcg ccagttggaa atggtgaata agtctttaac
    10561 cgaattagat acccagcttt cgcgcatgag tgaagaatcg caaatgtatt ctacacaaat
    10621 gaccgatagc caaatgactt tggaagaaaa gaaaattgaa tttgaacgca atatccgtga
    10681 agtcgaagac ctcaaagccc gtgtgtcggt tgaaaaagac acttacgagc aaatggcttc
    10741 tcagattcaa gagatgggcg acgaacttgc cagctcccaa agggctcgca atgaacgcca
    10801 atcccgcatg aatgaagcgc aactgaaact ggaacaggcg cgcatgaaag aacaatatct
    10861 tgtagatcaa atgcgcgagc gctatatgtt gattctttcc gatgtggctg agaaatatca
    10921 aggccgtgag ggggatcctg ccgaggccga agttcagttg aaagacttgc gtgaaaagat
    10981 cgcgaagatc ggtgaagtga atttgtcagc cattgaggag tacgaagaga ccgctcaacg
    11041 ttacgaattc ttgacgaaac agcaccttga cttaactgag gccaaggaac aacttcgcaa
    11101 agttattgat cgtattaata gaatttgttc taagcgcttt aaagagacct ttgatctggt
    11161 aaatgatcgt ttcacccgag tgttccccgt gttgtttggt ggcggtgagg cgaagttgga
    11221 gcttcatgag gatgtagaaa aaggcgaaat gggaattgag atcattgcca aacctccggg
    11281 taagaaaatg caaaatgtga ccctgatgtc agggggcgaa aaggctctaa cggcggtggc
    11341 cctggttttc tcgattttct tagtcaagcc aagtccgtat tgcttactgg atgaggttga
    11401 tgccccgcta gatgacgcca atgtattccg ttttaatgac ctggttcgtg agatggccaa
    11461 acgctcccaa attatcgtgg tcactcataa taaacatacg atggaagtgg cgggtaaatt
    11521 gtatggtgtc accatgcaag agcgtggggt ctctacaatg gtttctgtaa gccttcagga
    11581 tattcaataa atagaagaag ttgagaaaaa aaaggaagag aaaatgttcc gcgaattaaa
    11641 tgaagacaat gcgattttag tatttgcggt cgtgggagtg ctttgcactt tgatcttcgc
    11701 tcttctctgg tctttctttt taagaaagaa aagcgctcct ccacaggatg ctcaagaagc
    11761 tctggcaaaa agccttgaaa acaaacaagg gaagccactc agtgaacaac tgaaagagcc
    11821 agcgaaacct gaagtcgctg aagtcgatct gcgcaaggcc cttcgagcga ccgaagaaaa
    11881 tattttcggt cggattaaat ctctttttag ggcgaatgaa ccattggctc atcttgaaga
    11941 gattgaggaa attctctata ccagcgattt ggggcctcaa accgtgcaaa gactcatgga
    12001 tgctctcgag gacatgaatc gtaaagagaa agcggatctg gcaaaagttc gcgagatttt
    12061 gaaagcggaa atggaagggg ttttttcgaa acttccgagt aactcgattc aattcgcgaa
    12121 agaaggaccg acggtcctta tgatcgtggg tgtgaacggt gcgggaaaga ccacaagtat
    12181 tgggaagctt tcttctcaat ttgccagtca aggtaaaaaa gttctcgtcg ctgctgggga
    12241 tactttccgt gcggcggccg gggggcagct caaagtatgg accgagcgag ctcaggtgga
    12301 aatcttttgt cccgaaggtg tgacagatcc tagtgccgtc gccttcgatg ccgtgagcaa
    12361 ggctaaggga caaaattatg aggtggttat tatcgatact gcgggtcgcc tccataccca
    12421 agataacctg atggaggaac tgaaaaaaat gaagcgggtt atggcccggg tcattcccga
    12481 agctccccat gaagtgatta ttgttctgga tgcgaattct ggccaaaatg ctctgattca
    12541 agccaaagag tacaataaag ccctcaccct gaccggcgcg attttgacta aaatggacgg
    12601 caccgcgaaa ggcggtgtgg cggttggatt ggccaatgaa ctgcagattc ctattaaatt
    12661 tattggcgtc ggagagagaa tccaagatct tcgcaggttc tcttcccacg aatttgtgga
    12721 gagcatcttt agttaaaaag aaaacggagc gctagaaacg ctccgttttg gctttagggg
    12781 tccaaggggg ggggaggacg tcctaaatcc atttgataaa atttgaaagc actcattata
    12841 aacaaagcaa acattgtgac agtttcttct acgctatatt gaatccccgt aacgcttctc
    12901 ctgtaatgaa ttcagaactg ccaaatgaat aggtaattct ggaggaatga gtgacaaaaa
    12961 atgttcagcg tgaaacttac cgcgaagtaa gcttcttttt tatccttttg accttaaccc
    13021 tgcttgttgc cgttcaaggc tccgcccaag agggttcgcc tcgtcgttcc gcctcttctc
    13081 cttcgcaagt catggcagcg gctttttctt cttggaaagt gatgaagccc gaatggggag
    13141 ctgtcgaaga ggcgaattat atccgctatg ttactatggt gggcgaaggc gtcgccaacg
    13201 gtttatgtgc aacggtgtct gagtgttttc gcaactccag agtgaatccc tatgccttta
    13261 caaatcctgc cggattaagg ttcttttcag attgtgcgga ttttccctat ttcttacggg
    13321 cttactatgc ctggcaaaac caactgccct ttggattcgt agaatcagtg agtcctattc
    13381 aaggtcagaa tcaagttgtt aaagatattc ggtattcccc ctatggcaac atcgttcaaa
    13441 gacgttattc agtgcttgcg cgtaagtcca ttttaggacg ttctaagtat cccaatgcca
    13501 tagatatttt taataatatt cttccaaacc ttatttcttc agccaactat cgggtgagct
    13561 ataaagggat ggatacggat aatctttttt cggatttcta tcccattaaa atcacccgtg
    13621 acagtattca acccggaacg gtggtttatg atcccaatgg ccacgttgtg actatttaca
    13681 agatcaccag agatggacga ctttattaca ttgatgctca tccagacaac agtttgactt
    13741 cgggtctcta taactcacgt tttatacgct cttatccggg gcagggagct ggctttaaaa
    13801 attggcgtcc cttgcgtctg tctggagctt cgtgggatgc gactcggggt tactatggtg
    13861 gacgtattat aggcgcgaaa gacagagagc ttccgctcta tagcactgaa caatactacg
    13921 gaaccagcgc aagtccggat tggaaacaag cccaattcgc tttccaagga aaatcgatgg
    13981 attattacga ttgggtccgt aaccgattgg cggatgggcc gttggtggaa aatcctcttg
    14041 atgatataaa aatcatgacc caagaactct gcaatggagt gcaagaaaga gtcgtcgctg
    14101 tgcaagtggc gctcacagcg ggcttcaatc aacgcgagca tcctagccgt ctgccaaata
    14161 atatctatgg tgcggatggt gattgggaaa cctatgcaac tcccgccaga gatgcgcgtt
    14221 tgaaagtggc ttttaaagaa ctcagaacct atgtcgaaga ggctctccat cactataaat
    14281 cccatgatgg aaaagtggat taccatggag gagacctcgc cggcgatatg ctgaaggtct
    14341 acaagcaaac ggcgagtgag tgcccaatca cttataaaaa cagtgctgga cgacgagtga
    14401 ccataaatct cgaacaggcc cgtgaacgta tttacaagtt aagttttgac ccctatcact
    14461 gtgctgaact gcgctgggga gctgagggaa aagagttgaa ctcatgccgt gatagtagga
    14521 ccaagcgtct gtggtatgac cgtgaacaat ggcttcgcta tcagcatgaa agaaatacgg
    14581 acgctttcac tgggtttact gtcaatgaaa tcactggccc catgccagga gcgggggtgc
    14641 cctccggtca agatctggac gtgattgcat ttttatctag aaatcattag cattctccag
    14701 aggtcgcttt tagagagcga cctctgggag gacttttgcg agttttattt tttttggcat
    14761 tttgttttgt gtcggggagg gcttcctatt cgttcactga ccttgttcaa aaacctaaag
    14821 agaaacccat ctctgtttct tacatgaatt tgatcaaatg ttatcccgag ctaaaaaatg
    14881 agtctttggc gttgaaggta gacctaaatc agttaaaaag tgaaattgat agaaaatttg
    14941 tcacttctca atcgcttctt agatatcgtc aagtgattct caaggacccc aatggtctgc
    15001 aaaaacggct taaaatttca gcaaaacctg ccaagaaatc caaatttaat tatttgttga
    15061 gccttgaaaa attggactcc aaaggagctg gaactccatt tgagctgccc ctccctcaac
    15121 gccttaatcc ctctcagaaa gacttagacc aatatttctt ggatcaggat gtgttagagg
    15181 acgaacgttc gtactttgac actaagctca atggattgag tctgagcttt aagagaaaat
    15241 tcgacagtgt cttcgagttg gaattgagtg aagtttcaat gaagaggagg ctggtgtgtg
    15301 aagaccaaaa agacttaggt attgtctgta cttgttttca aaaataagtt tcaaataacg
    15361 cagtaatttg acaaacaaaa gttgctaaga ctgtgaagtc agctataatt tcagatactg
    15421 ttggtaaagg attttctaga atctttaatg aattctaaca gtttaagggc gccgagaagg
    15481 cgactgttta aagattgagc aggaccaatc atggtctacg aggtttacgt ggcatctgaa
    15541 aaaaaaaccc atgattttct tattgcggga gttccctata agttaaagac ctcccatgat
    15601 gatgcaactg ttcaagaact cgtcgaattt gtgaatcata aaatggacca agctttgggt
    15661 gtcactaagt atggttcctt tcaaaatgca gcggttctta ccgctctcaa tgtggcggaa
    15721 gagctgattc ttttgaaaag aaaagctcag cgcgaactcg agaagataga agaaaaagcc
    15781 cttaaactct ccattgatct agaaaattcc aagctcaaca gaacagttta actctgtttt
    15841 gtacgttgag gctggaatga atgtgtacgg acctaaaatg caatcgtggg gaaagcgtca
    15901 atggcgcgac cacttgaagg ctcttttgca aaaagagtct ccgttctcgt ctgtgcaaat
    15961 tcaaaatcag atttgcgaaa accttaaagg atacttacaa aacaaaaaag gaatttgggc
    16021 tggatatctg gccttgcctc aggagcccga tttaacttcg ctctatcgtc agggggagcc
    16081 tattgattgg gtataccctc gcatggaagc ggaacatttg gctttctacc tgtcgcatca
    16141 ttttatcaag ggtccttttg gagtgatgga acctcctgaa aaatctcaga agatccctct
    16201 tgaggagttg gtaggggttt tagttcctgg tttaggtttt aataaaaaag gtcatcgttt
    16261 gggaaaaggc cgtggttttt acgaccgggc tttggtggat tatcagggcc ctaaaattgg
    16321 gatttgtttt aattgccaag ttctcgagca ggatcttccg gaagaagaac atgacattcg
    16381 tgtggatgtt ctggtgacgg aaaaagaaat tatcaaatgc aatgagttta gtgtaatgag
    16441 gtaggagtcc cacatatgga agttgtaata gtcgcgattc ttggtttggt tttcggaggc
    16501 ttcgtagtct tcgtcatcaa aaaaatgcaa gatgagtcaa agaaaaagtc agcgcgttta
    16561 gaggctgacc gtattgtaaa cagggcgaat tctgaagccg cgaaaatcaa aaaagacgct
    16621 gaaacgaagg ccaaggattt tgaatcgcgc gcgcgtaaga atgttgagac ggatattcat
    16681 aaacaaaagt ctcagatgaa gaataaagag tctcagttgg accgtcgttt gaaagaggtc
    16741 gatgatcagt tcaaacagaa aatggaagaa aacgaacgtt atatcaatca gttgaaggat
    16801 cgcgaagaaa aagtcgcgat ctctgaaaac cgcatcaaag acctggaaaa gaaaacagaa
    16861 actcatattg gtgagctgaa gctgaaactg gagtcggtgg ctgcgatgac cgccgaagat
    16921 gcccgtcgcc aattgatttc agcgcttgaa gatgaagcga aggtggaagc ggctaaaaaa
    16981 atcgcgcaaa ttgaagaaga agcgcacaaa gaagctgaca aaaaagcgcg tcgtattatc
    17041 agcacggctc tttcccgttt tgcctctgaa tatacctcag aaagaaccgt cagcgttttg
    17101 gcgctgccaa gcgatgagat gaaaggtaag atcatcggac gcgaaggccg taacattcgt
    17161 actttggaag ccatgtgtgg agtggatttg attgtggatg atactcctga agctgtggtg
    17221 atttcaggtt tcgatccggt tcgccgcgaa ctcgcaagac gtactattga aaaactcatg
    17281 gaagatggtc gagtccatcc ggcacgtatt gaagaagttg tagaaaaaca aaaagcggag
    17341 atcacaaaat ccattaaaga agagggggat cgtgtggtgg tggagcttgg cattccaaac
    17401 atccatccgg aactgattaa attgatcggt ggtttgaaat accgttccac ccatgcgcag
    17461 aatgttttga atcattcact ggaagtggct tatatctctg gcctgctggc gggtgaaatg
    17521 ggcctgaact cgaaacaagc tcgtcgagcc ggaattttgc atgaaatcgg tcgcgctatt
    17581 gagcacactg cggaaggcag ctatgccatt gtcggcgcgg aatgggcgaa aaaatatggg
    17641 gaatccgaag agatctgcca tgcgatccga gctcatcaag atgatgaaaa accaaccacg
    17701 gctctggcct ggattgttca tgccgccaat atcttgtcgg cctctcgtcc tggagcgcgc
    17761 cgtccccaca tggattccta cattcaccgc ctcgaagatc tcgagagcat aggcaatagc
    17821 tttgacggcg ttttaaaaac ctttgccttg caggcaggac gtgaagtgcg ggtgatggtg
    17881 gagagcggta aggtcactga tgatcaatca gtgatgctgg cccgggacat cgctcgtaag
    17941 attgaacgtg agatgcctca atcgggtcag gtcaaggtca cggtggttcg tgagagtcgc
    18001 tctgtggaac atgcgaggta atcgatgtca ttcatggatc cacacgaaca gctcgagaga
    18061 ataaaatttg gtgccgccga gtttatcaac gacgctgata tgttgaaaaa actgaagcgc
    18121 agtaaagaga aaaaccagcc cttgcgaatc aagcttggcg cagatcccac gcgacctgat
    18181 attcatttag gccacactgt ggtgatcaat aaactgaagc tatttcagga cttgggccat
    18241 catgttcaat tcctgatcgg tgatttcacg gcgatgattg gcgatccctc agggaaaaac
    18301 accacgcgtc ccatgttgac ccgggaagag attgaagaaa atgcgcgcag ttataagacg
    18361 caaattttca aaattctgaa tcctgaaaaa actgaaatcg tttttaactc ccactggtgt
    18421 gagaaaatga atttcgcgga tgtgatccgt ttggcttccc gctatacggt ggcccggatg
    18481 atggaaaggg atgatttcac caatcgcttt aaatcaggaa gtcctatttc tcttcacgag
    18541 cttttgtatc ccctgactca ggggtatgat tccgtcgctc tgaagtccga tgtggaaatg
    18601 ggcggaacgg atcaaaaatt caatcttctt gtgggccgtg atttgcaaag tgcctatggg
    18661 caagaacctc aatgtgttct gaccatgccg attcttgaag gtctggatgg tgtcaataag
    18721 atgtcgaaga gcttggataa ttatatctca gtggtggata cgccaaaaga tatgtttggt
    18781 aagaccatga aagtttctga tgaactcatg tatcgctggt atgagctttt gacggacatc
    18841 accgctccgg ctttgacaca attgcaaaag gacgtgaaag agggccacaa gcatcctcgt
    18901 gacgtgaagg tccatttagc caagtttttg atcaagcgtt ttcactctga atccgcggct
    18961 cagcaagcgg aggaggaatt caatcgtatt tttgttgaga aaggtcttcc ggatgaagtc
    19021 ccggtgttcg aagtttttgc ggaaaatcaa ataggtcttt gtgctttgat ggtgaaagtc
    19081 ggcctggcaa cgagcaatag tgaagcgggt cgtttgatca gtggcggtgg tgtgcaaaga
    19141 gacggcgaga agttgtcgga tgcaaaagcc aagtgggatc ttaaaaccgg tgacgctttt
    19201 gtgttaagag ctggtaaaaa gaaatttgtg aaaattattg ttaaataaga tgcggatgaa
    19261 aaatgaaagt taaatttatt ccacaaaata ttgaactcga agggactcca gagaagagtc
    19321 tgttgcagat tgcaaccgaa aatcacatcg agatcaaatc tatttgtaag ggtgttccca
    19381 gctgtgcgga atgccgcgtg cgcgtggtgg aaggcgaaaa taatattctt ccgccgaaca
    19441 aagcggagct cagcctcatt gggacgaatt ggcgtattga tagccgtcgg ctggcctgcc
    19501 aaatgcgttg ttttggcgat gtgactgtgg atttaaccga acaactggag cgccaagaga
    19561 atcaaaataa aaaggtccgt ggattccgta gccaaaaaca aaaagagtct catgctgttc
    19621 aagataccat gctattaacg gaaaagcctg aagaaggaac catggagcaa gagccccaaa
    19681 ggcctccgca acagcaaaag cagcagcagc agcgccaaca gcaaggccgt caacaaggga
    19741 aacagcagaa ccagggacaa agacaaaacc agaatcaaaa tcaaaaacaa gggcaaaaac
    19801 agaatccaag gcaaaacccg aagcagcctc aaaagtcagg atctccaaac aatcctcaga
    19861 ctaagcaatc agaaaaggct caagacagga atcagaaacc taatcccaga gagtgagtga
    19921 ggatggcatg caaagaagtc gtagaacgaa tcttttagtt gcgggactgt tcggtttttt
    19981 aagcagcaca aacttggctt ggggtcgttc catgccaagc atgagaactt tacctgagac
    20041 tcatcctact tttagttatg atatcagtgc ttcgagcggg accgaaaata gcaatacgta
    20101 ctctgaatta aaattaaatt taaattggta cttgaatgac tggttgaact ggagaaacgg
    20161 tatttttagc cgttttggct ctaatattca ggcagtgaat ggtcttgatt cttccttgct
    20221 ggcagctttc gatacgagca ctgagggaga tcaatttggc gtgcaggtct tcgcaggtcc
    20281 aggtgtccgt atggcgtcgg cggataatag cgcagtcaca gcagaagcag ggattatctt
    20341 taagctcgga ggcattagtc tgggcggcgg tgcaaagtat ttatcttatt ttagtactcg
    20401 taaagatttg cttggaatcg tcctgcctaa ggatgaaacc caatatttta tagttctttc
    20461 tgggagtggc agtttctagc ttaaagtcta gttaacgtat ttctgttcac agccgaaaag
    20521 tgaatatggc tcataagatt acatttcgcg aacgcggaca gggtcgtatt ctcattctcc
    20581 tccatggttt tggcggcagc attcatcatt gggaatcttg tgctgaagag cttgcgaaaa
    20641 actaccgagt ggtggttgca aacctttccc atttattcat gagcacagac cgaatctttt
    20701 ttacagtgca agttgaaacc attgctcatt ttattaagac ccatttccct ggcgaaaaag
    20761 tctatattgc ggggctcagc tttggcggcg ctttaacctg ggctttgtcg cggcaacatc
    20821 ctgatctgat agaaaaaata gttttgatca atccggtgat tccaaatccg attccggagt
    20881 tttctttgcc agagcttcgt tatttctttg tgatgccgat gaactcacgg gcgatctatg
    20941 tcttgctttc aactccgatt ggcaaggcct tcctgaagcg ggcagcgatg attttccgtg
    21001 atgagcgcag tgaaggagtg ggtcgcttag acactctgaa agggcgacgt ttattattta
    21061 tttctcatct catctataat tttgcttgga tgcttcgccg tgaagactgg aactggtgga
    21121 aagacaaaat ttccatgacc agctctccct gtttgatgat ctatgataac caggatttgc
    21181 tgttctcaaa agaagtgtat cgaaaattca ccgacgagat ccgtgcgcaa aagtttctag
    21241 aaattagcgg agcgggtcac ttggcgatca agtcccagcc agaggtgatt tctgcagcga
    21301 ttcatgagtt tcttactaaa gccactgaac aggttgcttc atagggtcca aatttaaaat
    21361 aatgacgttt tgcttttcat cttatagagc ccctttggaa agtccagttt tctaaaatct
    21421 tatatttaac aagaagagtt tgtttgtatc attctatcaa tgagattttc aaaaatcctt
    21481 gagcgcgatt ttaaagatgt aatcttgatg gcaattgctg gatggctcat ggcctctgtc
    21541 ctaagtattt ccttgattgc cgaggcgcaa attccaaaaa ctctcaagtg ccgtggtgac
    21601 ttgcctatat tctgcaatga aaatagatct cgaaatccct tcaaactgtc taaaggattg
    21661 atcctagtac atcagcagga gtgtcaagct ttcagtgtcg gcggcacaaa agaaaagcct
    21721 ttcattagac tcagtccatg ggcggggatt caatatagaa caattcaagc gaacgtccct
    21781 catggcttcc aggtcaccct ttctgagagt tctgatgagg actttcatat tctcactgag
    21841 agtaaagtaa cgaaatttag aaaagagctt cactgttcct tgcagctgga tcttcgaggc
    21901 aagtatgaag ttaaataatt ttttaatttt cacgtattgt attttaatgg ccgcttccac
    21961 ctttgcaagg gttaaaggtc ccctggatgc ccccattcta aatgcagatc ctaagcttag
    22021 tacggatgaa aaattgggca ctggatttct attttctata attgataaag taaaaaataa
    22081 tcttccggaa ggaaaattaa gttgcgcaat taataaatat tcccaggttc tcggaatcga
    22141 gaagtaccaa agcacctacc atatgggaaa aacaaccgag gggtaggagc ctacttcgag
    22201 cacgaattga atgagaatcc ttgtctggct gaagttgcta gcaagtttgc cgtcacgctt
    22261 gcagttaaag ataacgaaag ttcagtaagt acaggttccc catcaagcct tcaagaaaaa
    22321 gccggtgagg gccgttataa aaatttttct ccaggttggg cctggcagca tgctctaaaa
    22381 tttaccaacg gagataagaa tgccgccatg tttttaattg gattatgtgc ctatgaggaa
    22441 gttccaatgt attcatttca agtgaccggg aaacacaagg tggctttgga ccggcgggat
    22501 atcgaagtcc tcgaagaacg tctgatgaaa gcaaaagcgg atctgaaatt tttccagtca
    22561 gaaaaaggtt atgagagtgc gaaagcatct acattatctg aagtgtcatt cattcaggaa
    22621 gcaattcgac aaaaaaaaac atcaatagaa aatcctaatc aaccagaaga gcattttctt
    22681 aaatgcccgg ataatggctc tgccttcact gctcctggaa gtctcggtgc aaacgtagat
    22741 atttcaggcg agttaaaaga aaaaatcaga caccttcaaa gacctcaggg aaagaatctt
    22801 gagaccttga aagcaaagta ttatcacatt acgggctctg catttatggg atgtcaactt
    22861 aagaagtgcg ggatgccgaa tatggtgacg aaactctggg agagccaagc gggcaggatg
    22921 tatcgtagca ttaggctctg tggccaggta aagaccaatc taaaaaatat tcaagtaata
    22981 tctaaaaaaa ttggtatgga tataaatgag cccaaatttc gtgaaactgc gaagctctat
    23041 ctccaagctg aggcgaaaaa gttgatagtc gaagggcaag accacgttaa gatgagggat
    23101 gccttatatg cacaaggtct tcgaggtccc gagttaacta agcgccttcc cagttacaga
    23161 tgcaatgatg caactctgag agaaacgaat ccccaagttg cgtatctttg tagtgcaact
    23221 tttcttcaaa gagaggcgga ctcaaactca gaggcagttg cgagagaagt cggagaaaca
    23281 gaccgagcga ttgacaggct tttgcaagaa tatgatgcct ctatcatgtt tagaaagtgg
    23341 tatatcggtg gagataacat tccatgcacc ggaattaagg ccgcaggccc tttccagtta
    23401 gatggaaaca atcctaattg tgaaatgtat ggctggtcgg cggaacgatg ctcaacggct
    23461 cttgcaaaac ttgggacctg ggatgtggat ttcgattgga cagtcgcaca acataaagtc
    23521 ggcgcggaat ttggtgccga gcaatgtaga gagcaagatc ctaatgaaaa actggataaa
    23581 acattttgca aaatactaga cgagaacaat aaaaaggaac tttatggcga agggaaggct
    23641 cccgcaccgc cttcggaaga gatccagggg gctaggtaag gcaaatatca cgtcgctccc
    23701 agcgatgcgt cactgttttt ttgttgtctt tctacaaggc ctgtctgtag attccgccac
    23761 acataacaaa ggaaattcaa tgaaatcaat gatcgttttc gtttctggat tgtttctttc
    23821 tttcaacgtg atggccgctg agaaaactct ttcgacggca attcctgcaa cagcctttgc
    23881 ttcgagcact atgacgaacg atgcactttt gccgagcacg ctggccttca attttgtggc
    23941 tttgtcacaa agtaaaacca acatctactt tgatgtgggt ggtttgagtg aaagagtggc
    24001 gcctgctttg agcttcagat cttattcaaa taaagaagta agaaaaaaat tgaacaatga
    24061 caaactcact gtcgatcgca gccttgcgac tcttggtgcc tctattgcgg ttcttaaggg
    24121 cgacgccaaa gccttgatct taaatccgta tctttacttc ggaactgaaa aagacgcagt
    24181 taatacagaa aatgtaaatg gcgtcggcgt tcgcttgtta ggtcaggcaa atgtttataa
    24241 agggcttggc ttccaagcgg gaattgacgc gaacaatatg gaaggcgaat tcaaaaacga
    24301 tctctatgtc ggtctggctt tcgcgcttta atttactaac gcatacaaat taagaataaa
    24361 aacccgagtc gccaaactcg ggttttttat tcttttctca agttaaaaat taaatcatta
    24421 aagcacggaa tataatctct aacaattcgt tggagaacct cggttgcgat atcctcttta
    24481 taagtatgtg aggtcaagtt acggtcttca atcatttgca gccaaaagtt ttcgctttct
    24541 ttggcgatcc aattattttg aaaagcgaac ataaaagctt ttttaggact tccgatttga
    24601 acaccttcgt gaccagcttt cttttgaatg ctcttccagc actgctcaaa ggtgtattca
    24661 aaggcttgga tgattcctgc tttgtcacgg tcattttgaa ttggaagagc tacgaagctt
    24721 tttagctgct tcaatgtttt ttcaaaattt gtgagttcat ccataaatca ctttgccctc
    24781 gacctgaata ttcttcctat aatcttcgcc gaggtcttga tataagacaa gatctactga
    24841 ataaagagaa agtgcttttt cgtctaactc cacctgaagc tgtgtccatg cttcaggggc
    24901 aatggaaccc ttcatggcga tatcgaaatc agatttttct ctatggtcac cccgagctcg
    24961 tgatccaaaa aggaccactt gttcgggatg cactttctca atcgcatatt gaataagatc
    25021 tttgattgaa ttaggcagtt gtgtgaaatt cttggtaaac gcaggcatgt taaacgttga
    25081 acgaagaacc gcagccgcag ttcttactgg cattaggatt attgaagaca aagcccttgc
    25141 cattcaagcc gccagaaaat tcaagagtca tgcctaagac gtaaagaata ctttgaggat
    25201 caacgacgac tttttgacca ttggtttcga aaaccttgtc gccttcacga gaaacggtgt
    25261 cgaaattcat tttgtaagac aagcctgaac aaccgccttt ttttacttcc acacgcaaaa
    25321 atgcagagtc actttgacct tcttcaactt taagaagagc caatttttta gcagcagatt
    25381 ctgagattgt gatcacaaaa gttctccttt ttaataagta acataatatc atagatgata
    25441 ctcgaggaga agtcccgagt actgattcac atttgcatgc taagttgctg aaattattat
    25501 gctaaacttt gtcggcgccc atggcctgag tcaggatctc cacggctata tctaattctt
    25561 cctttgtggt ccatcgtcca aggctgaggc ggatcgtgcc ttgggcttct tgtgatgaca
    25621 agccaatcgc ttttaatacg ggactggctt cgagacttcc ggtatggcaa gccgagcctt
    25681 ggctgaatcc taaacgaccg agtttcggta agagctgttc aatattttta ccaggaaaag
    25741 tcacattgag attgatgggt gaacgttccg taggatgacc attgagccga gccccgggaa
    25801 tatttttctg tattccggtc catagataat ttcttaactc agtggtgcga gccatttctt
    25861 cagagattat tttttggcaa agctctgcgg cggcacccag tccaacaatg gcagggacat
    25921 tgtgcgtgcc cgagcgatgt ccagactctt gtcctccgcc aaatatcaaa ggaggcagag
    25981 tcactttggg ttgattactt cgcatatata aaatgcccgc gcccttcggt ccgtaaaatt
    26041 tatgagcgga agctgaaaga aaatccaccg gaatctcatg catattcaca gggatcttac
    26101 ccagagcttg agtcgcatcg ctatggatat agattttttt ttccttcgct agcttcacaa
    26161 gatcttgcat agggttgatg ctaccaattt cattattgac ccaaataaag ctcatcaact
    26221 ttgtatgggg ctttattgcc ttggtcacgg cctcgatatc aagctggcca tatttattgg
    26281 tttttacaaa atcgacttca actccgcggc gttgaacttc cattaaagag tttcttatgg
    26341 aactgtgttc aacatcgctg gctaaaatgt gaagggggtc gcctggattt tcactttgga
    26401 atttattgac taaaccgaaa atcacccagt tgttgctttc ggtggcgcca gaggtgaagg
    26461 tgatttcaaa agactttgca tccaaaagag cagccacttg ggtgcgggct ttctcgacgc
    26521 ctttttgcgc ggcccaaccc cattgatggg caaagcttga ggggttgcca aagtgagtct
    26581 taaaataagg ctccatgatg gaaaaaacct gtggatccac aggcgtggta gcgttgtaat
    26641 ccaaataaat agggctggtc atattcaaca tgctttcatg ataacccttg cttgctctta
    26701 gagggaagct ctatctagaa tgaggcatgg caaaaaagaa attacaaaac ttagtttttg
    26761 cggagcactt tactgcgggc gtaaaagagg cagagaccct atttccttgg ggacgtcggg
    26821 actgtcttat caaagatcga cgcggcggta ttttttttga aatgaaaaat gtcgaggcgc
    26881 cgcagggctg gtctcagttg gccttggata tcgccgccag caagtatttc cgcaagaaga
    26941 cgggagtctt taagggcgaa cattctgtgc aacaaatggt tcacagagtg gtcgcggcca
    27001 ttgccaaagc gggaacccgc caaggcggaa tttttgccaa taaaaaacaa acagatattt
    27061 ttattaagga actcaaatat attttgtatt cgcagcgggc tgcctttaat agtccggtgt
    27121 ggttcaacgt gggattagat agctacggga tcgtttcgaa aagtgagcat tttgcctggg
    27181 acgaaaaatc aaaagctttc aaaaaaatca atgatgctta cgagagacct caatgctccg
    27241 cttgttttat ccaaagtgtg gatgactctt tggaaagtat ttttaattta gccaagtcag
    27301 aagcgaaact ttttaaatat ggttccggtt ctggaactaa tttttcacgc ttgcgcagca
    27361 agtacgagcc gctcttaagt ggtggcggaa caagttcggg acttatttct tttctggaag
    27421 ttttggatcg gggggcggga gcgattaaat ccggaggcac cactcgcaga gctgccaaaa
    27481 tggtgtgtgt ggatctcgat catccggaga ttttagattt tattgattgg aaaatgaaag
    27541 aagaaagaaa agctcatgtg ctcattgccg cgggttatga tgcggatttt gagggtgaag
    27601 cttatcgcac tgtcggaggt caaaacgcga acaattcggt gcgagtgcct catgttttta
    27661 tggaatcctt attagagcat aaagattggg agctaaaagc acgagtcagc ggtatggcca
    27721 ggaataaggt tcttaaaact ttgcctgcca aagaagtctg ggatcatatc gtgcattcca
    27781 cctgggtctg cgcggatcca ggggtgcaat atcatgatac catcaatgaa tggcatactt
    27841 gcccccaagc cggagggatt cgcgccagca atccctgctc tgagtatatg ttcttggatg
    27901 acagcgcgtg caacttggct tcgctcaatt tgacgaagtt tatgactccg gaaggggact
    27961 ttgatgtggt cagtttcatg cacgtggcac gcattatgtt tgtcgctcaa gaaattctcg
    28021 tggattactc cagttaccct acagaaaaaa ttgccgaaaa cagccatatg tatcgaccgc
    28081 tgggattagg ctttgccaat ttgggcagct tcttaatgag aaaaggactt ccttaccaca
    28141 gtgaccaagc tcgggcgtgg gccggcgcga ttgccagtct tatgcacggc atggcttatt
    28201 ggaccagctc ccaaatggcg gaaacccgtg gcccctttaa gggctatgca aaaaataaaa
    28261 aatccatgtt gaaggtgatc ggcagacacg ccgaagccgt gagtaaaatt ccttggacgt
    28321 tgttgccaga ttattttaaa gcgacctgtg aagagctttg gcaagatgtc gtggaaatgg
    28381 gaactcaata tggatttcgc aatgctcaag cgacagtgat tgctcctacc ggcaccatcg
    28441 gtctgttgat ggactgtgac accacgggca ttgagccgga tttttctctg ataaaatata
    28501 aaaaattagt cggcggcggg gaggttgaaa tcgtaaacca gtccgtgccc ttcgtcctgc
    28561 acctgttgaa gtattctgaa aaggaaattg aaagcattca ggcttatatc caagaacatc
    28621 gcacgatcga gggcgctcct tacttgaagg cagagcatct ccctattttt gattgcgcct
    28681 cggcccaaca agcgggccaa cgaactttgc caccggaaag tcacattcta atgatggcgg
    28741 cagtgcagcc ttttatcagc ggagcgatat caaaaacggt gaacatgccc ggctcggcca
    28801 gtgaaaaaga gatcagcgaa atttatcttc aatcctggaa gttaggcctc aaggccgtag
    28861 ccatttatcg agacggcagc aaagcaagcc agcctttgaa tattaaaaag gaagaggcca
    28921 aagcggggtt accagtcggg acgatgaaat gtccggaatg tggcgtggat acagtgctga
    28981 ccagtggctg ctatcgttgc ccgaactgcg gcagcaccgt gggttgcgcg taatattaac
    29041 gtggtattat tttcagaact tctttaagct gtctcatcgc ttcaagggta tgagacactt
    29101 caggtatgcg tggaacggag tgtcccgcat tataaattgc gcccttcaat gccagttcgc
    29161 aagctttctg cgattccctt actacatccg caaaaagttt ttcattcaat agtgtatcaa
    29221 tggcttttaa tcgcccatgg gctctatgaa tatagtcatt tcctaattta tgattgttca
    29281 ctgagaatct ccattttcca ggttcataaa aaggtgttcc aatgtaaatt aaataagatc
    29341 ctcaagtttc tgtaaccaga aaatcatcgc gcgctcagca aagtagagcg ccttgcgctc
    29401 agttattttt ggaattgtgg aaggagcttc gaggattgtt ttgaagctcg cccctcgata
    29461 agaaatctca gcgatggcac cggtcgtgcc ccaaggtctc aagatcattt ttagtacctg
    29521 tggatccgcg gaaaaattat tatgattgct aaaactccaa gtcgtagcac cggtggcgga
    29581 gacggggctt aagcgattga gcactgcctg tcctgaaagg gagtccacga cttgcagttc
    29641 tttgacgtga gttaattttt tggcgatcag gctggggacg tcttcgccat tgcgagcgac
    29701 ggtgacattg tggagagctt catccaggcg ttgaatggct ggcataagtt gtttcgttcg
    29761 cgattccaga aaagtcacct ttacttcgac ataaggaagg tgggcgcgat aacctttttc
    29821 gatttcaata cctcgaagac aatcttcagt gatatgggcc acgtcggatt ctcccagtcc
    29881 cagagtctcc caagagtaag tgatggattt atcgagatcc ttggtttttt cacgaagaaa
    29941 actgtcaagg ctggatttcc aaatcgccgc aatctcgttt gggggccctg gcaaaacaat
    30001 cacgtcctta ttgaatactc ttaattgaaa agcattggcc gtgccctctg aattcggcaa
    30061 aatccttgcg cctttcggga aaaaacactg ttgccgttgg gcttcgcgaa cgttaacacc
    30121 acgggatttg agtcgttgat tcacgtgctc ccacgaggct tcgtgaaatt ccaaagctaa
    30181 gccggaccac tcagaaatca gttcccgggt aaagtcatcc gttgtgggtc ccagtccccc
    30241 ggtgacaaaa agggtgtcgc aacgttcagc gcaaaagcga agagcatcaa gaatcagttc
    30301 tcggtcatcg ggaaccacga gatggcaacg agtgggaaca cccagatcct tcaaattttt
    30361 agaaatccaa gcggaattct tattaatgat ttgaccaatg gtgagctctg taccgatgct
    30421 tatgattgct acttgcattc tttatgtata gctcataact tattgcagcg aaatggcttt
    30481 aggcttgcgt tataaatgaa tcactcgcaa aaaagatggt tagaaagaac tactaaactg
    30541 taacaagaag gtcacaatgt ctgagttaaa agtggacgct cattggaaag aattcgatct
    30601 ttataatccg actcctgagc acgctatgtt gcgtgaaaca attgctaatt tcactaagga
    30661 agagatcgaa ccccaagccc atgaatacga tcgtaaagag cgttttaacc tagatctttt
    30721 tcgtaaggtc ggagagctcg gtcttttagg gattacagtt cctgaggcct ttgggggcgc
    30781 aggcatggac gcaacggcgg cggttttggt tcatgaagaa atgtctgctt cagatccagg
    30841 cttttgcttg gcttacttgg cgcattctat gttgtgtgtg aataatattg ccatgaatgc
    30901 cagcgatgcc caacgcagga agtatttgcc gcagctttgc tctggggaat ggattggctc
    30961 tatgtccatg tcagagcccg ctgttggcac cgatgtgatg gggatgcaaa ccgcggctgt
    31021 gaaaaagggc gacaaatata ttttgaatgg tcgtaaaatg tggatcacca atggaactat
    31081 tgatgaaaat aaaacccctt gtgatttgac tttggtttat gccaagacgg gcgagaaaaa
    31141 tggccgggcg ttgatttctt cttttttggt ggaaaaatct tttaaaggtt ttcaagtcgg
    31201 tcaaaagatt caagacaaat tagggatgcg gggctctaac accgcagaac ttgttttcca
    31261 agattgcgaa gtgccggttg agaatttgat tggcgctgaa ggcgactcca tgatgcatat
    31321 gatgaaaaat ttggagctgg aacgtctgac tttggcagct atgagcttgg ggatcgcccg
    31381 ccgctctgtg gagatcatga atcgttatgc cactgagcgc gaagctttcg ggaagtcctt
    31441 gaatttcttc ggccaagttc agcgctatat cgccgacagt tatgcggaat acaaagcggc
    31501 tcgcacttat gtgtacgaga cggcccggcg tatggatttg accaaagagg gcaatcgcat
    31561 ggactctgat ggcgtgaaac tcgtggccac caccatggct aagaatgtcg ccgatcgcgc
    31621 gattcaagtt ttgggtggct atggctatgt gggtgagtac gttgttgagc gtttatggag
    31681 agatgcaaag cttctggaaa tcggcggcgg taccattgag gcccatcaaa aaaatatcac
    31741 tcgtgatttg gcgaaaaatc cggaagcttt atacaagtaa tggagtccta tgttccctca
    31801 tccagaacgc atctctgttg atacgcattt gtcagttcat tatcaactga ttttggagca
    31861 catagggcct ctgctgacag cggaacgccg tcaaaaaatt gacagagtgg tggctggtcg
    31921 taactttgag accgccgtgg ttcttgaagg aatttacgat cgcggcaata gcagtgctgt
    31981 gatgcgaacg gcagaaggtt taggcttcgc caattttcat gtgattgaaa cccaggaaaa
    32041 gttcaaagaa tccaatcgtg tcactcaagg cgcagataaa tgggtagaga ctcaaaaatg
    32101 gaaacagagt aaagagtgca ttcaaagtct taaggaccaa ggctatcgga tttgcgtgac
    32161 cactctcgaa gccagtaagc ctttatccga agtcgatttc tccggaaaaa ccgctttggt
    32221 gctaggaaat gaaaaaaatg gggccagcca ggaaatgatt gctgcggcag acgaacggat
    32281 catcatcccc atgcctggct ttgtgcaaag ttttaatatc tcggtggccg gtgccttgtg
    32341 tttgtatcac atcatgcaag atcggattcg acgcaccggt agcaatgcgg atctgaccga
    32401 aaaagaacag gatatcctta aggctttgta ttatatgcga acccaggatt cagcccagga
    32461 ttatcttaaa gaaatgtttt ccagaggcac cttggcctaa tatatctcat aggaggaatc
    32521 atgcgtggtg gaataaaaaa aattgccgtg gcggtcctat tcactttggg cgggagtttg
    32581 aactcttttg ccagggagaa ggccttcgaa ctaaaaatgg agttaaccat caatggcaag
    32641 catatttcct ctccccgaat catcgccaag gaaggcgaga cagccatgtt tatgcaagag
    32701 gatcagactc aaaagacttt catcgaagtc gtcgccacgg aagataaaat cgtcagagga
    32761 aaacattctg ttttaatgaa atttgtcgtg ggctccatcg atccagatgg ccaaaagaaa
    32821 gtgatttata aaccacaggt tttgaccctg gtggatcaca aaacggattt gagcgtcgtc
    32881 gaagacatcg gcttgtcagt gaccgccagc cgagtctctc tttaagtctt tcgaataata
    32941 attgatttca tcagaggttc ttcgtctggc aattcaaaat ccaagcaaga gccaggggac
    33001 gcgagcaagg tgaagtggaa ctggcttttc aacttttgaa tattttttat aagatccgct
    33061 tgcgtccact tctcataatt ggtgcagaac agaagcaggc cgcccttgcc aaggcaatag
    33121 agacaattca ccaacagttc ttcataattt ttactgatac taaaagttcc gtttttggat
    33181 cttccgaaag agggaggatc gcagataatc atatcaaact tacgcttgcg gcgggccgtt
    33241 ccttttaaga acagtatgct gtcttgaacc cagaattcat agaggggctt cggcacctca
    33301 ttggacataa tggatttaaa gtccagcttg ggtttttcag caagagtgtc cgaatgagga
    33361 aggggaagac tattgagctc aaaattcttt ttgctccact caataaagtt actgctgaca
    33421 tcgacggtca cgacctcttt ggcgccccca agggcggcgt tgacgctgaa gcctccggta
    33481 taggagaaca gattcagcac agctttatct ttgctgtgct cacgcaccca acgtcgattt
    33541 tcacgttgat ccaaaaagag tcccggggaa agcccggtat cattgcggca atcgtatttc
    33601 actccatttt cacaggcaat ccaacgcaag ctttctttgc tgggccagag gatttgcgca
    33661 ttgggatctt ctcctcgatt gagcatttta cgaacaaaat attttttacc gaggcgtttt
    33721 gcgactcgct caaaacgttg cagatctttt tctgcaggat catgatcttt gtaccaatag
    33781 aaccagaggt aatcgccgaa cagatcaata cgataggaat caatgtcttg atgactcagg
    33841 cggaagcatt cgttgggctt tgcttggaaa ttcaaaagcc actggcgctt ttggcaggcc
    33901 tctaatatca tcaattcttc ttggtctatg ttgagatctt gctcaagttc tttcacccaa
    33961 accggtgcgt ccgcttgaaa gctgaacttt tgagatttgt attcgaagtt caggctctcg
    34021 gcatgcaagc acagacggta aaatggactg cccccatgtt ctctgcagcc cagaatagaa
    34081 atgccattgg cttgtgcctg caggcggatt tgatggggct tgcccgtggt gggttcggcc
    34141 aaccacaggt cgtaggctcc cagacttttg agcttggtga aggatgtttt agcattagga
    34201 gtcatattaa gaatatttac gaactgtccc ttgtcttttt caattctgga ctcatattcg
    34261 aattgatctc ggttgatttt tttatccgtg aggaagagat attttttttg aataagatgt
    34321 ttttcaaaga gcacagaaag ctccgccgcg ctttgcttat ttgtggcaaa caacatggcg
    34381 ccagaggtct ctttatcaag gcggtggacc acgaagagtt cttgctgtct tatttcagaa
    34441 agaatttcaa catacccttt tttgccaata tctggagaat gggtattaaa gcccgagggt
    34501 ttttgcgtga aaatgaaatc agtaggagaa gagcccatgt ccttcagcgt tacatggatt
    34561 tgattgtggg tcacggggag tttcagtaga ttgtattcac attttggagg attttaagaa
    34621 atgaaagcat tcataaaatt aatggcgttg tttttaactc tttcggttct tgcgggttgt
    34681 ggagttcagt cgattcctca ggctaagaac caggttgaag cttccttggc ggaagtcact
    34741 aatcagtaca aacgtcgagc tgatttagtt cctaatctgg tcaatgtggt gaaaggttat
    34801 gccaaacacg aagagcaaac tttgacctcg gtgattgaag cacgtgctaa ggcaactcaa
    34861 gtcacactgg atcccaccaa ggtcactcct gaaaaactca gagagtacca ggccgcgcaa
    34921 agtggtatgt cccaagcttt ggggagattg atggttgtgt cagagcaata tccgaactta
    34981 aaggcggata gccagtttag ggatttgcaa gcgcaactcg aaggcacaga gaaccgcatc
    35041 actgtcgctc gccaaagata tattcaggcc gttcaggaat ttaataacta tgtgacggtt
    35101 ccccccacta actggacgaa ttctctttta tatcattatg acaaaatgcc tcagtgggat
    35161 gtggctgaag atgaaaaagg caaagtagaa aaaccgcctc aagttaattt ctaataaaat
    35221 gacttatcgt ttattttctt ttctcttgtt gttttgcact ggcatttgct ccttagctcg
    35281 agctgatttt gaagtgccac gtttgacggg cccagtgatg gatgaggggg gcgtgattcg
    35341 tcctcaagat cgcaatgagc tggaacaggt cattcgggat tacaacagcc aaggcaaagc
    35401 gcagattcaa attttagtga tcgactcttt gcaaggccta gagattgaag aagctaccat
    35461 caaaattact gataagtgga agcttggaac cgagaaaaaa gacaacggta ttttattttt
    35521 aattgcgcct aatgagcgca aacttcgcat tgaggtcggg caggggcttg agggcacttt
    35581 gccggacgtc atagcaaaac gtatcatctc agacactgtt ttgcctctgt ttaaggcccg
    35641 caatctttca gcaggcatcg tggtgggtac ttaccagatc atcaaataca tcgatcaaga
    35701 gtatgcggat caacatcttt ctgagccagc acctgaatca cccaaaagta ttccgggctg
    35761 ggtgattttg gtgattttgc tcttgcttct ttttattgga cgcttcttgc cggcctcttc
    35821 tttccgaggt ggtggcttca gaggcggtgg atggggtggc ggcagtttcg gtggcggcgg
    35881 cggtggtggc tggtccggcg gcggcggcgg tggtggctgg tccggcggcg gcggtggttt
    35941 tagtggaggc ggagcttcgg gctcgtggta atttatgaaa caaagatggg ttacaaaata
    36001 tctcactgaa aaacaaatcg cggaaatcag ccaagccgtg catcaagccg aagggggaac
    36061 gagcggagac attgtgccga tgatagtccg tcgcagctct gcggttcgtc atgtgccggt
    36121 gatcttgggc ttgagttttg cgttgatttt ttttggcata gagattctgg gtgtgaatgg
    36181 gtggtatata agtgattttt ttccctttct gatggggcac tgggtttatc agatcggctt
    36241 cttagcactc ttgatggtgt tagcttgggg actttcgcat cttcatccgg tgcagagact
    36301 tttgacctct gacgaggatg aaaaagagca ggtctggaga cgggctcaat tagaattttt
    36361 tcttcatcgc ctgcaccaca ctcacaaaaa gacgacggtc ttgatctttg tctccgtcat
    36421 ggaaagacgg gccgtgattt tagcggacga ggccgtcgct aaggttctac ctgcaaatac
    36481 ctgggaagaa gttttaaagc ccttgacggg atttttgcac aaaggggagt ggtctgcagg
    36541 cttccaggac tctatccgtc gcagcggcgc acttctcaag gctcattttc ctgagcctgt
    36601 gggaggtact cctgccaacg aaataagcaa taatctcatc atcaaagcct agaagtgaat
    36661 ctcgtgacat tcagcagcgg tcttgcgtca ttaagcgctg gaaaaatctc aactgcgatg
    36721 ataaacaatg gtggtcctta ataaatcgaa atgcggccgt agctcagctg gatagagtac
    36781 ttggcttcga accaaggtgt cgggagttcg aatctctccg gccgcaccaa attggcaagt
    36841 attcgaacta taatgaccca agaattgcga tgcgttacct gagtttaaat caggtaacgc
    36901 atttttttta tccaaatcgg attttacggc atttgatgcc gctgaggcct ttctattctg
    36961 attttcaccc tgaccgtgct taattaaaat atcttcacat aggattcgcc gaccttccga
    37021 ttactcactc gcgacaaaat taatcgcatc aactcctgtt taattcataa atcacttttc
    37081 aaggccaagg atcacacaag tccatgctcc tgacgaaatt tactcaccag ttggccttta
    37141 tcggcttagt tgttgcaatt tctcattttc atgaagtgtt ctcgctttgg accgaattgg
    37201 atgcttaaga ttttgacggt gctgtgcctt tctggggctc tatcctgctt tgcctcagca
    37261 aatatgtgtg cccagctctt ttctccatta tctttcactc caagtcactt tgcttcgtcc
    37321 acgttcggac tcgtacatgc tgaacgtccc gggcaggccc gatattcgca atggtccgca
    37381 gacaccgcta atgaggtgct ccttgccatc gctgcaggaa agcccgcaaa ggaacttttc
    37441 gaaatcggag ttcgagcccg gcaggagttc gctcgcaaat tcgaaaacgt ggataaatct
    37501 gaagaagact ttggggcctc cagggtgacg agaagccgca acatcgtggg cgaagatatg
    37561 attggcctca gttccttgac cttggatcga agaagaggtc aagctttgcc agcccacttc
    37621 gacgcttggc ctaatccctg gtgggccaat attgcgagaa cagaaagccg cacccaactc
    37681 gctgactcaa ttctgataca cctggtggaa actcaacgcc tgagcacgac ggattacaat
    37741 cttctgatct gggatcgttt ttaccagact tcaactggtg aagtcttaaa atcctcaagc
    37801 acgaagattc aacgcgcttt tcctgagcag caggatagtc cctattttgt gagcacgctc
    37861 aatgcaccaa aaccggagat tgaactcctc atgactgacg tctatcgccg gattcaactg
    37921 gctttaaaag caccgaccaa aaaggaagcg cgcgaaaatc tgagtcatgc gatgtacggt
    37981 tttttcaatg ccatgcctta ctaccgcgga tcagctgcaa ttggtcggat cgttttcacc
    38041 gtgctctttt catcaattga ggaacgaccg atgattattg atccggaatg cgatgtaaaa
    38101 gccctcgttc aaagccaatt cgaattcctg acaaatattc aatccatcgt ccgcactggg
    38161 aagtaagatg cactcgtcct ctgttcggca tttctcttaa aaaatccatg agcggttctt
    38221 aaaaagaaat gaattccgac aatcatttat gagtagggga cacctggaag aagatctgtc
    38281 atgtgcgagt tgtctgagtc atgaaattcg tccaagcagt gtagtaagtt gttcgcatcc
    38341 atatatttga gccgcaactt taaagatcac tgagttacca ttcttaaggc ctatcatttt
    38401 aatgccttct ggaagttgtg gattctttaa aatcaagaca aattcatacg tggaatttcc
    38461 ttggtggcag cgaattatat caggtagtga ctgatcttgc tacttttata cttctggata
    38521 aactgataga ccgagtcgtg cgatacgccg aagctctttg aggcttcacg ataggtcatg
    38581 ccatcagcat gtacttctga gaatgctttg gttgccttcc cgggggtctt ttttctttta
    38641 ttcttggggg tgcaaatact tccatctttc ggtccatttc tgcgaaagct tctatcaaag
    38701 cttcgcggat aggccaaatc attcttgctc attaactatt taagccgctg tctgaccggt
    38761 ggggctcacc ccaagtctgt agaaaagaag aatctaatgc ccaataactt tgcgaccaat
    38821 caaacccttt ttaacatttc attgttgacc tatcatcaac aatatcaaaa gattgccaag
    38881 aggtttgcat gaaaagattt ttgattttct ccttagtttt ctccttagtt ttgtcctttg
    38941 gaacggcctt atatgcgcag gatctgttaa atagcataca agctcctgaa gaaatgactc
    39001 ttctaagtac ttcaaaaaca gagacgttga atctagggat ctccctatca tcgcaaagta
    39061 attctaaaat tcttaagaag gtatggaaaa cagcctcaga gaaggcttgc agcgctgaga
    39121 tgcaaaaatc attttcagag gaagctcgcc ttaagctgga gcgacaatta aaatcagaag
    39181 atattatcga tgatctcgcg gcagttttga atcctttttt attttcttta ggcatgtccc
    39241 ataccgagtt tatgactgcc accagcgaga gctattattt ttttaaaagc tatgaagcac
    39301 ttcttcatcc cgagcaaaga cctgcgcctc atatattaaa tcctggtgtt caggtcggca
    39361 aagataaaga cggatactac gcgagagaag tcttaacagg ctttccggca gaccttgccg
    39421 gattaaaaaa aaaggaccgc ctagtttcct tggatggcca ggctttttca ggaacctggg
    39481 gtagttctcc aaaagtggct ttagtgacgg tacaacattt gggtcagctt tcgaatttgc
    39541 ctatagaaat taaatccctc gactggaatc agtcttttca agatgccacc aaaagcagtg
    39601 tccagatttt cactgggaaa aacggaaaga aaattggtta cattcactta tggaccggcg
    39661 tacaccccca gagtgctcgc tttttaaaag actctgttct caatctaaaa cgacaaaaaa
    39721 tttcaagtat aattttagat ctacgcggag gatatggcgg cgcttggtgg gagcacttgg
    39781 aggcattcta tccagactcc agcacttaca tgcaactaga ggtcatggcg acggacggag
    39841 aaactgaaat tctgaaaagc ccctttaaaa agaataagaa ctatttttcg gggcctatgg
    39901 cggtattgat caatgaaggg gttcgctctg gtaaagaagc cttggcccat caatttaaaa
    39961 agacaaaaag agcctatctc attggtgaaa aaaccccggg gtatttttca acaggacagt
    40021 atttttacgt gaacgagcct gttgattacg ctctttacct ctgcgtatat caacttaagc
    40081 tagatggaaa aattcttgag ggcataggca tcgaaccaga tgaggatgtg atttacaaac
    40141 cgagtggccc ttttggagac tctcaattag aagccgctgt ggattatcta tccaaatagc
    40201 atgattttaa ccccttagat agtgctcgtg acctaaatca atagcatatg aatcctgatt
    40261 gcttaaagtt tggcagtcgt cagcaaggca cgggattcga gtgccctatg aataaaatct
    40321 agatttgctg atgtaggatt aggcaaacat gtccatgttt tttacagcta taaacaaaaa
    40381 tgagctaatt ccaatccctg ccttgttagc aagaccgggc gtgaggtact cgcttggctt
    40441 aatcttggca taccccttgc tctaaagaga ataagcctac tattgcagtt aaaaggatgt
    40501 cgtgtgaaag tcttgtataa gaaaagcttt atatccttaa ttttgttgat gccggttttt
    40561 cccatcctga ctttggctca ttcaggcatt tctcaactta aagaaggtga cgtccttgaa
    40621 ttgcaaaaac gtggccgaaa tgcgagagta agcccgaatt tttccaagca taataaatca
    40681 aacttagcat ttggctcaga ctcccgttta ggaccaggaa ctttggttcg ctatacgggt
    40741 aaatatgagg ggaccaaaca gggtaacttt ggtattcagg tggagatatt gcctcaggaa
    40801 gggaaaccct ttgatgaaag gcatcgaggc aagctggttt ggatctacta tagaaatcac
    40861 gagcgaagta tcgttgaaaa acctggcgaa gtagcgacag ggccaattaa tccagcagac
    40921 atcactgagg aaaaaaatct tatccgccca gatgaatcac ctgaggattt ttccgaaaaa
    40981 aattctgtag ctcaaaccga agacaacgaa gtccctgaag tgatacagga ggctcagaat
    41041 actctagata aaattggcaa gataaattcg gggttaagct gcaccaatag tacatgccgc
    41101 gaagaggata acgattccgc tcgtccgctt caagccaccg atgctctttt aaattatgaa
    41161 ttaggggagg ctctcacttc ctcttgggcc caagaggaag tattaaaggc tgggattcct
    41221 tcgacacctt tgttgcgggc tttacatttc tataatgcga ataaagattt gaggaatcta
    41281 cagagaaatt atttggtggt ggcagattta acccaagctt caaataaaaa gcgtatgtat
    41341 aaaattgacc tgaggagtgg caaagtttca aaatacacca caagccatgg cagaggctat
    41401 ggcaatgcct attattgcgc gaatactttt ggaaatagaa gggacagtaa cctttcatcg
    41461 ggaggaggct atgtcaccgg cccatcccgc tcttttcgtg gctccaaagc actgaatttg
    41521 catggagtgg aggtgggtaa gaatgatcga gctttttcaa gaggagtcct tttacatagg
    41581 gcctcctatg tcaatgacca tcaggcgggc cgaacttggg gatgtttatc cattgccgat
    41641 aaatattcta aggaatttat tagtagccta gagggtcatg gttctaatag tggttttatt
    41701 ggtcagggct ccgcgatata tgtctatcct gatcgcgatg atattcttaa taaaaattct
    41761 tattgggacc cctcctgtcg ggctaattta aatgcccaag gcgaccgata taagccgcgt
    41821 tggatacctt agactcagct ataatttctc tctttctcat tagcctgacg gctctccgtt
    41881 ttatagaagg gtcttaaatt ttctactaga cgaagatacg gctatcataa gtcgcgaagg
    41941 cgcttattat tcattttgaa actcgtagcg cgactttact tctgtttgag tacaaccaga
    42001 agtccaatga
//
LOCUS       DATBTE010000327         1930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1888557, whole genome shotgun sequence.
ACCESSION   DATBTE010000327 DATBTE010000000
VERSION     DATBTE010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888557"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VIG33_12385"
     CDS             complement(<1..564)
                     /locus_tag="VIG33_12385"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEY8271679.1"
                     /translation="MNKLTNYQQTILYQLIEKVTGGHQQGFSQKKIFVSNVENLMREA
                     GKTFEEYLDLIGKDPGEFSKFLASVTIHTTSWFREIEHFSILEEKVRERLQAGQSSFK
                     IWSAAASSGQEAYSIGLVFQSLKSEFPQLEYKIWGTDIDGLSIEKAKGGVYPIGELKQ
                     IPKKYAYWVLQGENKAQNFFTIDPEIRL"
     gene            complement(574..>1930)
                     /locus_tag="VIG33_12390"
     CDS             complement(574..>1930)
                     /locus_tag="VIG33_12390"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEY8271680.1"
                     /translation="PQELRKFVFLGSGSVKGLMTFDPEIRKRVKFFQNNLAETPYKTP
                     LDGYDLVFCRNVLIYFERKVQEAIVHGIVNKVLNSGILIFGHSDSFPSNRDIVSIGRS
                     SYKKEVKTKAESISQRRKRILIVDDSTTIRKVISKILTADFLIDESDSAVAADIALDN
                     NKYDLITLDLNMPGENGHSWLTRHRKAGLKTPVVIVSDSSPSEAEKVFGALEAGAQDY
                     IVKSRLQSEPEKIVELLKSLTETRDTYLLQGHTFRPFVNKRHSPRILLLGASTGGPEA
                     LVKVLQGFPKPCPPIVVVQHISPEFSRAFASRLAQLSGLNFSEIEEGQVLQPNSLYTA
                     TGDYHLILKEKQEKLFLLKSNEPKVNGHRPSVDVLFNSAAKLPVDSLSILLTGMGKDG
                     AEGLFNLAETNRSFTLVQDEKSSVVFGMPKKALELGAACFVGDLSAIRAEMINKINGG
                     K"
BASE COUNT          546 a          469 c          358 g          557 t
ORIGIN      
        1 cagtctgatt tcaggatcaa tcgtaaaaaa attctgagct ttgttttcac cctgcaaaac
       61 ccaataggca tatttttttg gtatctgctt tagttcccca attgggtata cgccaccttt
      121 ggctttttca atcgacagcc catcaatatc cgtgccccaa atcttatact ctagctgggg
      181 aaattctgat ttcaaacttt gaaaaaccaa tccgatggaa tacgcttctt gaccgcttga
      241 agccgccgca gaccagatct taaaagaact ctggcccgct tgaaggcgct ctcggacctt
      301 ttcttcaaga attgaaaaat gttctatctc tcggaaccag ctcgtggtgt gaatagtgac
      361 ggaagccaag aactttgaaa actcgcccgg gtctttgccg atcaaatcaa ggtattcttc
      421 aaaagttttc cctgcttccc tcatcaaatt ctcgacattg gagacgaaaa tttttttttg
      481 gctgaaaccc tgttgatgtc caccggtgac cttctcgatc aactggtaca agattgtttg
      541 ctgataattt gtaagcttat tcattaaact cacctatttc ccgccgttga tcttattgat
      601 catctcagcg cgtatcgctg acaaatctcc aacgaagcac gccgccccta attcaagcgc
      661 tttttttggc atgccaaaaa caacactcga cttttcatct tgaaccaaag tgaaagaacg
      721 attcgtctct gcgagattga atagaccctc cgctccatct tttcccatcc cggttaagag
      781 aatagaaagg ctatccaccg gcaatttcgc cgctgagtta aacaagacat ccacactggg
      841 cctatgtcca ttgactttcg gttcattgga tttcaacaaa aataattttt cctgtttttc
      901 cttcaatatt aaatgatagt ctcctgtagc agtatacaag gaattgggct gcaatacttg
      961 cccctcttcg atctcactaa aatttaaacc cgatagttgg gccaagcgac tggcaaaagc
     1021 acgggaaaac tccgggctaa tatgctgaac caccactatg gggggacaag gcttaggaaa
     1081 gccctgcagg accttaacca gggcttccgg accaccagta ctggcaccca acaacagaat
     1141 acgtggtgaa tgccttttgt tcacgaatgg acgaaaggta tgcccttgta aaaggtaagt
     1201 gtcccgggtt tccgtcaatg acttcaacaa ctcaacaatt ttctcaggct cactctgcag
     1261 cctggatttt acaatataat cttgcgctcc cgcttccaaa gcaccgaaga ctttttcggc
     1321 ttcactgggc gaagaatcac tgactatcac aaccggtgtc ttcaatcccg ccttacggtg
     1381 ccttgttaac caagaatgac cattttcccc gggcatattt aaatctagag tgatcaaatc
     1441 gtatttattg ttatcgaggg ctatatccgc agcaacggcg gagtcgctct catcaatgag
     1501 gaagtcagcc gttaaaattt tactgatcac tttacgtata gtcgtcgaat catcaacaat
     1561 caaaattctt tttcgacgct gcgatatgga ttcagctttc gtcttaactt cttttttata
     1621 ggaggatctc ccgatggaaa caatatctcg attggaggga aaagaatcgc tgtgcccaaa
     1681 gattagaatg ccactattaa gaaccttatt cactattccg tgaacaatag cttcttgcac
     1741 cttgcgttca aaatagatca atacattacg gcaaaaaacc aaatcatagc catctaaggg
     1801 agttttatag ggagtctccg ccaaattatt ttgaaaaaat ttaactcttt ttctaatttc
     1861 tggatcaaaa gtcattaaac ctttcaccga tcctgaacct agaaaaacaa atttccgtaa
     1921 ttcttgaggg
//
LOCUS       DATBTE010000328         1812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2367326, whole genome shotgun sequence.
ACCESSION   DATBTE010000328 DATBTE010000000
VERSION     DATBTE010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2367326"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..509
                     /locus_tag="VIG33_12395"
     CDS             99..509
                     /locus_tag="VIG33_12395"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEY8271681.1"
                     /translation="MDLKQGDLENIILNALWDLEGEGSDKIFVGDIQERIKSANKKWA
                     YTTVKTVLDRLVDKEIAVREKDGKKYLYRSMVDRDEAGVLALKKVVRQYFKNDVNELV
                     SCLNRLQTPSKIITERKNAARQLENTRREIAGRS"
     gene            738..989
                     /locus_tag="VIG33_12400"
     CDS             738..989
                     /locus_tag="VIG33_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271682.1"
                     /translation="MQIMFQACSKTRFGSDEWRRDGFPNEAEHGRYKNWAAQKGLNKY
                     GDPKDMVYMGGDPLFYSRMSLYQYASTNHPQKPWNEKAK"
     gene            complement(1060..1596)
                     /locus_tag="VIG33_12405"
     CDS             complement(1060..1596)
                     /locus_tag="VIG33_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271683.1"
                     /translation="MTGIYPAPSTASVQPFLARKVTQQSILFSGAVTDKVNIARKKVQ
                     EEAERAIFERANLVLDEAFFTVGETEVTGREVLKLIDEMGKTCECKVGKALSSESNTY
                     YPWNVQSKLAPLVEAGLITVGTRVVPKNGYLFHHSLKLTERGREVAYASASYSEPADA
                     VEPPSTLLEADSTNYPED"
     gene            complement(1628..>1812)
                     /locus_tag="VIG33_12410"
     CDS             complement(1628..>1812)
                     /locus_tag="VIG33_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271684.1"
                     /translation="RWGFMDGLKGLFFMHQTVSLTEKGRKEANARPEASTWEPPPPEV
                     PLLKPLDGDTTNYPGQ"
BASE COUNT          451 a          418 c          473 g          470 t
ORIGIN      
        1 ttgaaagttc aacaacattt caacaaaggt agagcggcag tttttgttca acgcatcacc
       61 cgaagtataa aaacagtatt tggaggacga ttgaagggat ggatttgaag caaggagatc
      121 ttgagaacat catcctgaat gccctgtggg atttagaggg agaaggcagc gacaagattt
      181 ttgtaggaga tattcaggaa agaattaaat cggccaacaa aaaatgggct tacaccaccg
      241 tcaaaaccgt cctggatcgt cttgtggaca aggaaattgc cgtgcgtgaa aaagacggca
      301 agaaatacct ctaccgctcg atggttgacc gcgacgaggc cggtgttctg gccctcaaaa
      361 aggttgttcg ccagtatttc aagaatgacg tgaatgaact ggtgtcgtgc ctgaaccgtt
      421 tgcaaacccc ctccaagatt attaccgagc gcaagaatgc tgcccggcaa ctggaaaata
      481 ctcgccggga aattgccggt cgttcttaac ccccccaagc taatccggtt tgtttgtata
      541 cgagcgttca agtggcttta gtcagggctt gtctttagca ctgctgatat cattcatatt
      601 gaaacttgtg acgtctaagg ctggctcagt ctggctttgg ggtgttgtaa gttttcaatt
      661 tttggcttac gcttcttaac ataaattccg gtatacattt ggttgattag ttgagagaga
      721 caaaaggggt attttccatg caaattatgt tccaggcatg ctccaaaacg cgcttcgggt
      781 ctgatgaatg gcgtcgcgac ggatttccga acgaggctga gcacggcaga tataaaaact
      841 gggctgccca gaaggggttg aacaaatacg gcgaccccaa ggatatggtt tatatgggag
      901 gggacccgct gttctattcc cgcatgtccc tgtaccagta cgccagcaca aaccacccgc
      961 aaaaaccctg gaatgaaaaa gcaaaataga tagagacagt attgaattga gaagaccgcc
     1021 cttgcaaggc ggtctttttt tgtctgtttc gttggaagct tagtcttcag gataattggt
     1081 gctgtcggct tccagcaagg tggatggcgg ttccacggca tcggcaggtt cggagtatga
     1141 tgcggaagca tacgcgacct cgcggccccg ttcggtcagc ttcagagaat ggtggaagag
     1201 gtagccgttt ttgggaacta cccgggtgcc taccgtgata aggcccgcct ctaccagtgg
     1261 ggccagcttc gattgtacat tccagggata gtaagtatta ctttcactgc tcagagcttt
     1321 tcccaccttg cattcgcagg ttttacccat ttcatcgatt aatttcagca cttcccggcc
     1381 ggtcacttcg gtttcaccca cggtgaaaaa ggcctcatcc aggaccaaat tagccctttc
     1441 gaagatggcc cgctccgctt cttcctgcac tttttttcgg gcgatattga ccttgtcggt
     1501 cactgcccct gaaaagagga tactttgttg tgtaaccttc ctggccagga atggctggac
     1561 agaggctgtt gaaggtgctg gataaatacc tgtcatttca gaactcctgt tgaacatttc
     1621 ataaaattta ttggccggga taattggtgg tatcgccatc aagcggcttt aacaagggga
     1681 cctccggcgg agggggctcc caggttgaag cctctggtct ggcgttggct tcttttcggc
     1741 ccttttcggt gagtgagacg gtttggtgca tgaaaaacag tcccttgagc ccgtccatga
     1801 aaccccaccg ga
//
LOCUS       DATBTE010000329        12809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2436141, whole genome shotgun sequence.
ACCESSION   DATBTE010000329 DATBTE010000000
VERSION     DATBTE010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 12809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12809
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2436141"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            588..923
                     /locus_tag="VIG33_12415"
     CDS             588..923
                     /locus_tag="VIG33_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271685.1"
                     /translation="MRKIFSIILLTSTLSSATFASDLFAHQCAHKAVQAALAIAKING
                     VKGGLMLPSKVLKTKLLAASEDYLSVAMFYVGNKKGDASFSYKVEIGDASELGGSSCE
                     VMAVFIPQP"
     gene            1171..3045
                     /locus_tag="VIG33_12420"
     CDS             1171..3045
                     /locus_tag="VIG33_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271686.1"
                     /translation="MSVGLAIFSLQAPAAVESVALDYVALDNSVDCHRTFFRSSQHFK
                     GTIFAVPRVLQGNGVYAATGLESTRIFNILPSKNPGFYDLTVYLYFPANDESARGSKA
                     SLFKKDNEACNWDEAIAAINKNIADPKDWVKVASNIPLTAIEMKIPGFARAGRVGTFS
                     SIEEPSILEYYGKSISVHFEITELERQLFESQVVSYGGIDASIKMYFRARSRTGSVNA
                     KIDTEGLVSNFSAEAKAKGMKYVGEANLAALLTSAVQKTSIEIHGEEGSSSESLGKVT
                     LQIIEKVFKEVSLLPVDTAAKKTASAKDPSSVVDVSAVMDILKKRLSGTISFEMFSAS
                     EVASAQREVNLQTERLKDPNLNEVTVKAQYLDPSSGVSLNAGETITIVPAYWYIEKFE
                     YREKKVYLSETDLKDLRLDEHFSDLTDERMHIANRTINNNSVAIGSWYPLLFGDKVQL
                     PYRFRWLRVLREASNSRISSNEIPTTYEALKELPVFLSFSKLGDRKFTRLSNLMGDNE
                     FWNAVYDTQTGRIILTAKIDLGTIRFRERIRGKENIEVGSTPVILDEVFEIMHSPFGR
                     KSTRDYHMIKSDAKPMIKQKAIVFNVSRPMKLAGKELEYVKALRKANSTPLIEESSPE
                     "
     gene            complement(3053..4072)
                     /gene="trpS"
                     /locus_tag="VIG33_12425"
     CDS             complement(3053..4072)
                     /gene="trpS"
                     /locus_tag="VIG33_12425"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY8271687.1"
                     /translation="MSEELSKKPVILTGDRPTGPLHLGHYVGSLVNRVKLQDTYQQYL
                     IIADSQALTDNFDNPQKIRDNILQVALDYLAVGLDPNKSTFFIQSQIPELYELTMHFM
                     NLVTVSRLMRNPTVKEEIKQKNFAESLPAGFFVYPVSQAADILAFKTDLVPVGEDQNP
                     MIEQTNEIARRFNHLYKTNCLKEVKPLIGSTGRLLGIDGKAKMSKSAGNAILLSDSAD
                     IIKAKVQKMYTDSNHLRISDPGQVEGNVVFSFLDIFDPNKTELEELKAHYRRGGLGDG
                     SLKQRLTDVLEDLIAPIRKRRQDYEQDPAEVMSICMKGSVKARECAQQTTKEVREVMA
                     LIPLL"
     gene            4240..5319
                     /gene="prfB"
                     /locus_tag="VIG33_12430"
     CDS             4240..5319
                     /gene="prfB"
                     /locus_tag="VIG33_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_142699827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEY8271688.1"
                     /translation="MRSLGTSFGGIFDLDKKQKRLAELAMAVENPAIWEKPAEMQKLN
                     KEKTLLEKAVGEWTSYSRRLEDAKVLLEMAVEGHDEASFSEVKTETLAIEKIGQVLEL
                     SRLLNGEMDSNSTYLSINSGAGGTESCDWAEMLMRMYTRYAEHHGYRVEILDITEGEG
                     AGIKSCTLLIEGPYAYGYLKAESGVHRLVRISPFDSNARRHTSFASVFAWPEVDEDIN
                     IEVRPEDLRVETFRSSGAGGQHVNKTDSAVRMYHLPSGIVVSCQVQRSQIQNREKALK
                     MLKAALYEKEVEKRNAEKDAMNSVKKANEWGSQIRSYVMHPYQLVKDHRTDFETNQVE
                     DIMNGELDNFIMAYLKSNFSQAVNS"
     gene            5320..6549
                     /locus_tag="VIG33_12435"
     CDS             5320..6549
                     /locus_tag="VIG33_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8271689.1"
                     /translation="MKQLFWIAYRLLFSKRTLFGGSAVLSLIGLILGVGALVASMAVM
                     SGFESTLRSAMADVTGHVQLVKRGTFQEPWQELEARIRKQEPSLVSSVRFLRTEAVLA
                     HQGVIQGVMIQGIDEERAGDVLNFSSRLVDGSLELKGGTDEKSPALIGKRIAQRMRLK
                     VGDTFRVVMPIPDPIDPGHFQRRIAEFQVRGILDMGKTEWNERMIISTLATAQKLVQI
                     GDRYSGLLLKFQDINYARTAAFNLSQSLGPHYWVSDWRDLNENLFEAVKIERVVIFFV
                     VLIIVVVAAFNVSSTLFVNVVQRYADIAILKALGLPQKSILKMFSLQGILMGAVGLAF
                     GTVFGLLLCVGFTLLQNTMGLLSGSVYRIDAIQVEVRFVDWIFIALATMLICFVATLA
                     PALRGSKLSPVEGLKYG"
     gene            6542..7228
                     /locus_tag="VIG33_12440"
     CDS             6542..7228
                     /locus_tag="VIG33_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8271690.1"
                     /translation="MVSPAPALIRADSIVKAYPQGDGGELQILRGLSLDISHGEAICI
                     LGASGAGKSTFLQILGTLDRPQSGQLFFHERNLLEMNDEDLALFRNEKMGFVFQFHHL
                     LAEFTALENVMIPCRIAGQSVRDARDKATQLLYQIGLGSRTDHYPSQLSGGELQRVAI
                     ARALVRHPQILFADEPTGNLDMQNSQRIQDLFFELKDRLGLALVVVTHDLAFAQKFPK
                     ILRMKDGQWV"
     gene            7384..9765
                     /gene="bamA"
                     /locus_tag="VIG33_12445"
     CDS             7384..9765
                     /gene="bamA"
                     /locus_tag="VIG33_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEY8271691.1"
                     /translation="MSPNLLLAQPAKRPSARAKKSAPTAAKPAAALTPERVVKSVDVS
                     GMKKLEKDAIINRIVSKPGEAYSDEKLREDVKALFKSGYFVNVEVDREIKGADVSLIY
                     RVTEKPSIVEIVYEGNSEVKSEDIAETSGIKAYEILNMSKIKEATDKIQKLYEDKGFF
                     LAKIDPVVEDVKKEESVRVVFKIKENDKVKVKKITILGAKKLSENTLKSSMITKEGGY
                     FSFMSGSGAYKQEAFDRDTQALRYVYYNQGYIQAKIDRPQVYVTPDKKSIYITMRVEE
                     GEQYNVGEIDFGGDVLFAKEELFETIKLTSGKVFAYDVLYKDLSDLQAKYGDLGYAFA
                     NVIPRTRINDKDLKVDLLFEFDKGNKVYLGQINVVGNSKTRDKVVRRELKIREGELYN
                     ETRRRQSLENVQRLGFFEEVNFKTSTSPDKPDILNMDIMVKERNTGQIQLGAGYGTVT
                     GFTLQGSVNQTNFLGKGQNLGASLNISDTGSYYNFTFTEPYFQDTLWSLGSELYQSID
                     SGRSDYDEKRTGGALSLGHPIGEDWRGILRYKLDKTQLDPKYVGGVSITDPVVFPLET
                     ANGYTSSLTTTAQYDTRNDRFMTSKGVLTSLSYEYAGIGGDLKYQKATGIFRYFKNIF
                     WDVVWRNSLTYGKIESLDSSKNPPFNQLYLLGGPYSLRGYRPYRVGKQVYSQTAYNNA
                     LTNIPTPTDEQKANANVLAQVFYGGQQQLMYQTELQFPLITEAKIMGVVFYDIGQAED
                     QLVSDRFFADMGFGIRWFSPIGPLRFEWGFPLNRDPVYHKDTMIFDFSIGAPF"
     gene            9835..10341
                     /locus_tag="VIG33_12450"
     CDS             9835..10341
                     /locus_tag="VIG33_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEY8271692.1"
                     /translation="MKKQVTVIASLMLAAGLVHAAEAKIGVVDMQKAIQSTAAGKKAK
                     AELEGEFNKKKKELEKKEADLKKMGEDLERKKSVLSEEVLSKKQAEFQEEMLKYRDVV
                     GKSQIEIQKKERDLTAPILEKMKKSIEKVAKEKGYTLVLENSQMVLFATADADLTNDV
                     VKSYEKEK"
     gene            10381..11193
                     /gene="lpxA"
                     /locus_tag="VIG33_12455"
     CDS             10381..11193
                     /gene="lpxA"
                     /locus_tag="VIG33_12455"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEY8271693.1"
                     /translation="MKIHPTSVVSPDVEIGPEVEIGPYCLIQGKGKIGKGTFVEGHVT
                     LGYKHGVILIGENNHFSPGAVIGGPPQDISYKGEETSLIIGNNNIFREFSTVNLATSK
                     ADKKTEIGNNCYIMAYTHIGHDCKLGNNVIMANNSQLGGHCEIESNVVISAMCGFNQF
                     TRVGRGSFVAGGSVVNKDIPPFVRAEGHWAVVRATNKIGLIRKGFSREEANNVHKAVR
                     ILVMGKGTVEENIERISAECMHSENIEHFIHFIKSSKRGIAIARNPKEVADE"
     gene            11186..12142
                     /locus_tag="VIG33_12460"
     CDS             11186..12142
                     /locus_tag="VIG33_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY8271694.1"
                     /translation="MNNQMSSQLRAAVVGVGYLGTFHAQKYAKNTQAQLVGVCDFNSA
                     QAAKVAADLGVAAIPRAQDLIGQVDVVTVAASTQSHYELAKLFLQNGIHVNVEKPITA
                     TIPQAEELVNLAQQKNLKLAVGHIERFNPSIVEFKKHLKNTKTLELTRLATYKTRGAD
                     VSVLHDLMIHDMDLLYWLTGSEIESFTAAGSKLISKELDTAQVALKMKSGTMVYIHVS
                     RVFSSIQRWIRATQKESILYANTGTLELEKSEAAPISGVGGDTPVKVTNWTVTKEDAL
                     QKETDAFIDAVLNNKPVAVSGLDGWKSLKAIEDVKATIEAQL"
     gene            12139..>12809
                     /locus_tag="VIG33_12465"
     CDS             12139..>12809
                     /locus_tag="VIG33_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEY8271695.1"
                     /translation="MNEVMIVAAEASSVIYAQRLLEHWQREGKKIKAFGVGSSEMEKL
                     GFERIGNAEEMAVVGAAEIVSAYSRLKKVFDNLVIAAAARRPKVVIVMDYPEFNLMLA
                     KKMHALGIPVVYYISPQVWAWRKGRVKTIKKYCKKVFVLFPFEVPFYEEHQVPCEFVG
                     HPILDELDDKLFDESYRKTHRNQCGIGDHEMVLGLMPGSRNLELKQHFQIQLEVARKL
                     MKKYQ"
BASE COUNT         3533 a         2539 c         3164 g         3573 t
ORIGIN      
        1 gtccattagt aaaatggatt caactaacat tttaccaatt tcttgattca atgagtcgat
       61 agacttcttt ccccaactga ttgtttgggc aactttcaac gcgaaattgt cttcgcccaa
      121 taattcttgg aaatgacttg cagatgcttt tctttgcttg gtatttattt tcatgaggga
      181 agcctctctt tctttttaag cagtggaaat tattccacga aaggttggct ccctcaactt
      241 tcaactagat ttgagatttc tccttcagtc ttctagtaat aaatttcaca gtaccattgt
      301 aactcaaact caaaatattt cgactgtttt agagataatg tatggggatt tccgacaact
      361 ttacggaaca gggcctgttc aaccgggcta ttttgatggc ttagtgagtg ttggcaatat
      421 ggtcagagat aatgccaatt cgtattcaac ctattcaaat gaactgaatg accagcttac
      481 taagttaatt attgatttag gctcttgcta ttcaactaag tttactgcgg cgaaataagt
      541 taaaagacgg ttcatttttt attaaaacat tatcatagag gacatatatg aggaaaatat
      601 tttcaattat tttgctaacg tccacccttt catctgcaac ttttgcaagc gatttatttg
      661 ctcatcaatg cgctcataaa gcggtgcagg ctgccttagc tattgctaag attaatggag
      721 tgaaaggtgg tcttatgctg ccatccaaag tgttaaaaac aaaacttttg gcggcttccg
      781 aagattactt atcagttgcc atgttttacg tgggaaacaa aaaaggcgat gccagtttta
      841 gttataaggt ggaaattggt gacgcctctg aacttggcgg aagttcttgt gaagttatgg
      901 cagtttttat accacaaccg taattaaaaa ttgtttgaga gagaatcgtg gacttataga
      961 catcgtgagc ggctacagtt ttccgacgct ttgtgtagtt tctagacgcc ctttgatctt
     1021 caaatagaaa taaacccgat ttcggagtgg catcaatgtc gcaaattgat ttttccgtac
     1081 tagttaattg ctacgggcct tattaaagaa agcggagttc tgttatgcgt gggaattttc
     1141 aaaaaacaat aagcatctct aaaatccctg gtgagcgtag gtcttgccat attttccctt
     1201 caggcccctg cggctgtgga gtccgtagcg ctggactacg ttgctttaga caattctgtg
     1261 gattgccatc ggactttttt tagaagctcc caacatttta aagggactat ttttgccgtg
     1321 ccgcgggtcc tgcaggggaa tggtgtttat gccgctactg gcttggaatc cactcgcatt
     1381 tttaatatcc tgccttcgaa aaatccgggg ttctatgatc tgacggttta tttatatttt
     1441 cctgccaacg acgagtccgc cagaggctct aaagccagtc tgtttaaaaa ggataatgag
     1501 gcctgtaact gggatgaggc tattgctgcc attaataaaa atattgccga tcccaaagac
     1561 tgggttaagg tcgcttccaa tattccttta accgcaattg agatgaaaat tcctggtttc
     1621 gcacgggccg gtcgtgtggg aacttttagt tccatcgaag aaccaagcat tcttgagtat
     1681 tatgggaagt ccatttctgt gcattttgaa atcaccgagt tggagcgaca gcttttcgag
     1741 tcccaggtgg tttcttatgg tgggattgat gcgtccatta aaatgtattt ccgggctcga
     1801 agccgcacgg gttcggtgaa tgccaaaatc gatacggaag ggttagtgag taatttttcc
     1861 gctgaagcaa aagcaaaagg catgaaatat gtcggtgaag ccaatctggc ggctctgtta
     1921 acaagtgctg ttcagaaaac ctcgatcgaa atccatggcg aagagggctc ttcgagcgaa
     1981 agtctcggaa aagtcacact gcagattata gaaaaggtgt ttaaagaggt gagtctcttg
     2041 ccggtggata cagcggcaaa aaagaccgcg tccgcgaaag atccttccag cgttgtggat
     2101 gtctccgctg tgatggatat tctgaaaaag cgtctgagcg ggacgatcag ctttgaaatg
     2161 ttctcggcct ccgaagtcgc gtcggcacaa agggaggtga atctgcaaac ggaacgtttg
     2221 aaggatccaa atttaaatga agttacggtg aaagcacagt acttggaccc ttcaagcgga
     2281 gtttccttaa atgctggaga gaccatcacg attgttcccg cttattggta catcgaaaaa
     2341 tttgaatatc gtgagaaaaa agtttatctc tctgaaactg atttaaagga tctgaggctc
     2401 gatgaacact tttcagatct aacagatgag cgaatgcata tcgccaatag aacaattaat
     2461 aataactcag ttgccatcgg ttcatggtat cctttgcttt ttggtgataa ggttcaattg
     2521 ccctatagat ttcgttggct gagagtcctc agagaggcca gcaactccag aatatcttca
     2581 aatgaaattc ccaccaccta tgaagcactt aaggaactgc cagtgtttct cagcttctcg
     2641 aagttgggtg atagaaagtt cactcgtctt tcgaacctga tgggggataa tgaattctgg
     2701 aatgcagttt atgacaccca aaccggcaga atcattttaa cggccaagat tgatttagga
     2761 acgattcgct tccgcgaaag aattcgtggc aaagagaata tagaagtggg ttccactccg
     2821 gtgattttgg atgaggtttt cgaaattatg cattctcctt ttggcagaaa gtccacgcgg
     2881 gattatcata tgattaaatc agatgcaaaa ccgatgatta agcagaaggc gatcgtattt
     2941 aatgtcagcc gacccatgaa actggcgggt aaagaacttg aatatgtaaa ggcgcttcga
     3001 aaagcaaatt cgactccttt gatagaggag tcgtcaccgg aataaaatta cttcataaaa
     3061 ggggaatcag cgccatcact tcgcggactt ctttagttgt ttgttgcgcg cattcgcggg
     3121 cctttacgct gcccttcata cagatgctca tcacttcagc ggggtcttgc tcatagtctt
     3181 ggcggcgttt tctgatgggg gcaatcaagt cttcgagcac gtccgtgagt ctctgcttga
     3241 ggctcccatc ccctaacccg ccacgtcgat agtgagcttt gagctcttcc aattcagttt
     3301 tattcggatc aaaaatatcc agaaagctaa ataccacatt gccttcgact tggcccggat
     3361 cgctgatgcg cagatggttc gagtccgtat acattttctg caccttggct ttgatgatat
     3421 ctgcggagtc actcaataga atcgcattgc ctgcggactt gctcatcttc gcttttccat
     3481 cgatgccgag gagtctgccg gtggaaccga taaggggttt gacttcttta aggcagtttg
     3541 tcttataaag atgattaaag cgtcgcgcga tttcattggt ctgttcaatc atgggatttt
     3601 gatcttcgcc gacgggcact agatcggttt tgaaagccag aatatcggcg gcttgactga
     3661 ctggatacac gaagaatccc gcaggcaggc tttctgcgaa atttttctgt ttgatctctt
     3721 ctttgactgt gggatttctc atcagtcgtg agactgtcac tagattcata aagtgcatgg
     3781 tcaattcata aagttcaggg atttgtgact gaataaagaa ggtacttttg ttcggatcca
     3841 atcccacagc gagataatcc aaagcgactt gcaaaatgtt atcgcggatc ttttgaggat
     3901 tgtcgaaatt gtcagtaagc gcttgagaat ccgcgataat aaggtattgc tgataagtat
     3961 cttgaagctt cactcgattc accagagagc ccacatagtg gcctaggtgc aagggacctg
     4021 tggggcgatc gccggtcaaa ataacgggtt tctttgaaag ttcttcgctc atatctgagg
     4081 cttcctcgat tttctaaaaa taatgtataa taatgcatag gtaaatttat gccaaattat
     4141 tgactccagc gacctggact atacactggc ctttcttata agggaaaatc atgtcaattg
     4201 ttacagagtt atccgacatt aaaagaaaat taacggacct tgagaagttt gggaacgagc
     4261 ttcgggggta tctttgacct agataaaaag caaaagcgct tggcggagct cgccatggcg
     4321 gttgagaatc ctgcgatctg ggaaaagcct gcagaaatgc aaaaattaaa taaagagaaa
     4381 actttgcttg aaaaagccgt gggggaatgg acctcttata gtcgtcgttt ggaagacgca
     4441 aaggttcttt tggaaatggc ggttgaaggc cacgatgagg cttcctttag tgaagtgaaa
     4501 acggaaactc tggctattga aaaaatcgga caggtgcttg agctcagccg tttgcttaat
     4561 ggggaaatgg attccaatag cacttatttg tctatcaact ccggggccgg cggaaccgag
     4621 tcctgtgact gggccgagat gttaatgagg atgtacaccc gctacgcgga acatcatggg
     4681 tatcgtgttg aaattttaga tatcaccgaa ggggaaggtg cgggaataaa atcctgcacg
     4741 cttttaattg aagggcctta tgcctatggc tatctgaagg ccgaatctgg agttcatcgt
     4801 ttggtcagaa tttctccttt tgactctaat gctcgcagac acacttcttt tgcctctgtg
     4861 tttgcatggc cggaagtgga cgaggatatc aatatcgaag ttcgccccga agacttgaga
     4921 gtggaaacct ttagatccag cggtgctggt ggacagcacg ttaataaaac ggactccgct
     4981 gttcggatgt accatttgcc gtcagggatt gtggtctctt gccaagtgca acgcagtcag
     5041 attcagaatc gtgagaaagc cctcaaaatg ttgaaggccg ctctctatga aaaagaagtt
     5101 gaaaaacgca atgccgaaaa agatgccatg aactcagtga aaaaagccaa tgagtggggt
     5161 tcacagattc gttcctatgt gatgcaccct tatcagttgg tgaaagacca tagaacagat
     5221 tttgaaacca accaagttga ggatattatg aacggcgagt tggataattt tattatggct
     5281 tatttaaaat ctaactttag ccaagcagtg aattcataga tgaaacaact tttttggatc
     5341 gcgtatcgct tgctcttctc aaaacgaacc ctgtttgggg gttcggccgt gctttccctg
     5401 atcggattga ttctgggggt gggggctttg gtggcctcca tggcggtgat gagtgggttc
     5461 gaatccactt tacgctcagc tatggctgat gtgacaggtc atgtgcaact ggtgaagcgc
     5521 gggactttcc aagagccctg gcaggaactt gaagcgcgta ttcgtaagca agaaccttcg
     5581 ttggtttctt ccgttcgttt tctccgtacg gaggccgtgc ttgctcacca aggggttatt
     5641 caaggtgtga tgattcaagg gattgatgag gagcgcgctg gcgatgtact taacttttcc
     5701 tctcgtttgg tggacggaag tcttgagctt aaaggcggca ctgatgaaaa gtctccggcc
     5761 ttgattggca agcgcatcgc tcaacggatg cggctcaaag taggtgatac ttttcgcgtg
     5821 gtcatgccaa ttcctgatcc tattgatccg gggcattttc aaagaagaat tgctgagttt
     5881 caggtgcgcg gaattttgga tatgggaaag actgagtgga atgagcgtat gatcatttcc
     5941 actttggcca ccgctcaaaa gttggtgcaa atcggggatc gttacagcgg tttgctgctg
     6001 aaattccaag atatcaatta tgcgagaacc gcggctttta atctgagcca gtccttaggg
     6061 ccccattatt gggtgagtga ttggcgggat ctgaatgaaa atctttttga agccgtaaaa
     6121 attgaacggg tggtgatttt cttcgtggtg cttatcatcg tcgtggtggc ggcgtttaat
     6181 gtctcaagca ctctttttgt aaatgtggtg cagcgctatg cggatattgc gattctcaaa
     6241 gctctaggac ttccgcaaaa aagcatttta aaaatgttta gtctgcaagg gattttgatg
     6301 ggggcggtgg ggctcgcttt tggaactgtc tttggtttat tgttatgtgt gggatttacg
     6361 ctcttacaaa ataccatggg acttctgtcg ggatctgttt atcgcattga tgcgattcaa
     6421 gtggaagttc gttttgtaga ttggattttt atcgctcttg ccaccatgct tatatgtttt
     6481 gtggcaactt tggcgccagc attgcgtggc tctaaactca gccctgtgga aggattgaaa
     6541 tatggttagt ccagcacctg ctttgattag ggcagattcc atcgtaaaag cttatcctca
     6601 aggggatggg ggagaattac aaattcttcg cggcttgtct ttggatatta gccatgggga
     6661 agctatttgt attttaggag cctcaggagc gggaaaaagc acttttctgc agattctggg
     6721 gactttagat cgccctcaaa gcggacaact tttttttcat gaacgcaatt tgcttgagat
     6781 gaatgatgaa gatttagccc tgttccgcaa tgaaaaaatg ggctttgtgt tccaattcca
     6841 tcatttgctg gcggagttta cggctctgga aaacgtcatg attccgtgtc gtattgcggg
     6901 tcaatccgtt cgcgacgccc gtgataaagc cacccagttg ctttatcaaa tagggctggg
     6961 ctccaggacg gatcattacc cttcccagtt gagtggcggt gagctgcaac gggtcgctat
     7021 tgccagagct ctggtccgtc atccccagat ccttttcgcc gatgagccca ctggaaacct
     7081 ggacatgcaa aacagtcagc gcatccaaga tctctttttc gagcttaaag accgtttagg
     7141 cttggccttg gtggtggtga cccatgactt ggcctttgcc caaaagttcc caaaaattct
     7201 ccgcatgaaa gatggccagt gggtctaatg tttgacaaga cataggtctt gggctaggct
     7261 ttccttttat ttttgtaagt atttaaaatt attacgaatt ttactgaaat tcttcatgaa
     7321 ggaaaagcta ttttgcgatc aagatggata ctccaaaaat taattttagt tttaattttt
     7381 ttcatgagcc cgaatctgtt gttggctcaa cctgcaaaac ggccctcagc aagagctaaa
     7441 aagtctgcgc cgacggcggc gaagcctgca gcggctttga ctccagagag ggtggtcaaa
     7501 agcgttgatg tgagtggaat gaaaaaactt gaaaaagacg ccatcatcaa ccgcattgtc
     7561 agcaagcctg gcgaagccta cagcgatgaa aaactacggg aagacgtaaa ggctcttttt
     7621 aaaagcggct actttgtaaa tgtcgaagtg gaccgagaaa tcaaaggggc tgacgtcagt
     7681 ctcatttatc gggtgaccga gaagccttcg attgtagaaa tcgtctatga gggaaattct
     7741 gaggtcaaaa gcgaagacat cgcagaaacc tccggcatta aagcttatga aatcctcaac
     7801 atgagcaaaa tcaaagaggc gacggacaaa attcaaaagc tctacgaaga taaaggtttc
     7861 tttctcgcca agattgatcc ggttgtggag gacgtaaaga aagaggagtc ggtccgagtt
     7921 gttttcaaaa ttaaagaaaa cgacaaagtc aaagtgaaga agatcacaat tctgggcgct
     7981 aaaaaacttt cggaaaacac tcttaaatca agcatgatca ccaaagaggg cggctatttc
     8041 tccttcatga gtgggtcagg ggcttacaag caagaggctt ttgatcgaga cacccaggcc
     8101 cttcgctacg tttattacaa tcaaggttac attcaagcga agattgatcg tcctcaggtc
     8161 tatgtgactc cggataaaaa gagtatctac atcaccatgc gagttgaaga aggtgagcag
     8221 tacaatgtgg gcgagatcga ctttggcggt gatgttcttt ttgccaagga agagctcttt
     8281 gaaaccatca agctgacgtc aggaaaagtt tttgcttatg atgttcttta caaggatctg
     8341 agtgatctgc aggcgaagta tggggactta ggttatgcct ttgccaatgt gattccgcgc
     8401 actcggatta acgacaaaga tttgaaagtg gatttgttgt ttgagtttga taaaggcaat
     8461 aaagtttatc tgggtcaaat caacgtggtc gggaacagca aaacccgcga taaagtggtc
     8521 cgtcgtgaat taaaaatccg tgaaggcgaa ctttacaatg aaactcgtcg tcgccagtct
     8581 ttagaaaatg ttcagcgtct gggattcttc gaagaagtga attttaaaac ctccacttca
     8641 ccggataaac ccgacatctt gaatatggat attatggtga aagaacggaa caccgggcaa
     8701 atccaattgg gcgcgggcta cggaaccgtg accggcttta ccttgcaggg ttccgtcaat
     8761 cagacgaact tcttgggtaa aggccaaaat ttgggagcgt cgttaaatat cagcgatacc
     8821 ggcagttact ataattttac cttcacagag ccatacttcc aggacacttt gtggtccttg
     8881 gggtctgagc tttatcagag tatcgattct ggacgttctg attatgatga aaaaagaacg
     8941 ggtggagctt tgagtttggg acatcccatt ggcgaagact ggcgcggcat tcttcgttac
     9001 aagctcgata agactcaact ggatcctaaa tacgtaggtg gagtttccat tacggatccc
     9061 gtggtctttc ctctggagac ggcaaatggc tacacaagtt ctttgaccac cactgcccag
     9121 tacgacacgc gaaatgatcg gttcatgacg agcaaagggg ttttaaccag tctttcttac
     9181 gaatacgcag gcattggtgg ggatttgaag tatcaaaaag ccacggggat cttccgttat
     9241 ttcaaaaaca tcttttggga tgtcgtttgg agaaactccc tgacctatgg aaaaatcgag
     9301 agcttggatt cctcgaaaaa tccccccttc aatcaattgt atttattggg cggtccttat
     9361 tcattgcgcg gctatcgtcc ttatcgtgtg ggtaaacaag tttattcgca aacagcttac
     9421 aacaatgctt taacgaatat tcccacaccg acagatgagc aaaaggccaa tgcgaatgtg
     9481 ctggctcagg ttttttacgg gggccaacag caattgatgt atcaaacaga attgcaattc
     9541 ccgctgatca ccgaagctaa aattatgggc gtggtattct atgatattgg gcaagctgaa
     9601 gatcaacttg tgtccgatcg cttttttgcg gatatgggtt tcggaatccg gtggttctca
     9661 ccgatcggac cgcttcgttt cgagtggggt ttcccgttga atcgggaccc tgtctaccac
     9721 aaggacacca tgatctttga tttctcaatt ggggcaccat tttaaattca cataaaaaat
     9781 ataatattaa gttaatattg agtttagtaa ccataaggat tttttggagg atagatgaaa
     9841 aagcaagtaa cagtgattgc aagtttgatg ttggccgctg gattggttca tgcggcagag
     9901 gcgaaaattg gtgttgtaga tatgcaaaaa gcgattcaaa gcacggcggc tggtaaaaaa
     9961 gcgaaggcag agcttgaagg cgaatttaat aaaaagaaaa aagaacttga gaaaaaagaa
    10021 gctgatttga aaaagatggg cgaagacctg gaaagaaaaa aatctgtttt gtctgaagaa
    10081 gtcttgagta aaaaacaagc tgaattccaa gaggagatgc ttaagtaccg tgatgtagtg
    10141 ggcaaaagcc aaattgaaat ccaaaagaaa gagcgtgatt taacggctcc gatcttggaa
    10201 aagatgaaaa aatccatcga aaaagtagcc aaagaaaaag gctataccct cgttcttgag
    10261 aacagccaaa tggtgctgtt tgcaactgcg gacgctgatt taacaaatga tgttgttaaa
    10321 tcttatgaga aagaaaaata gtttttcatt tttattccct gctaactaga ggagtccgcc
    10381 gtgaaaatcc atcccaccag tgtggtttca cccgatgttg aaattggtcc ggaggttgaa
    10441 attggccctt attgtctgat tcaaggcaaa gggaaaatcg gtaaaggtac tttcgtggaa
    10501 ggccatgtaa ctttgggtta caaacatgga gtcatcttga ttggagaaaa taatcatttt
    10561 tctccggggg cggtgatcgg cggtcctcct caggatattt cctataaagg cgaagaaacc
    10621 agtctgatca ttggtaataa taatatcttc cgcgagtttt cgactgtcaa tttagccacc
    10681 agcaaggccg ataagaaaac ggagatcggg aataattgtt atatcatggc ttatactcat
    10741 ataggtcatg actgtaaatt aggtaacaat gtgatcatgg ccaataattc tcagttgggt
    10801 ggtcattgcg agatcgaaag caacgtcgtg atcagtgcga tgtgtggttt taatcaattc
    10861 accagagtgg gacgcggctc ttttgttgcc ggggggtccg tggttaataa agatattcct
    10921 cctttcgtca gagcggaagg ccattgggcg gtggtgcgag ccaccaataa aattggcttg
    10981 attcgtaagg gcttttctcg tgaagaggcg aataatgtgc ataaagcagt tcgtatattg
    11041 gtcatgggta agggcactgt ggaagaaaat atcgaacgca tcagcgcaga gtgcatgcac
    11101 agcgagaaca tcgagcattt tatccatttc ataaagagtt ctaagcgggg cattgccata
    11161 gctcggaatc caaaagaggt cgccgatgaa taatcagatg agtagtcaac ttcgcgccgc
    11221 agtggtcggt gttggttatt tagggacttt ccatgcgcaa aaatacgcta agaacaccca
    11281 ggctcaactc gtgggagttt gcgacttcaa ttccgctcaa gccgctaaag tggctgcgga
    11341 tctgggtgtg gcggccattc ctcgcgcgca agatctcatt ggccaagtgg atgtcgtgac
    11401 ggtggccgcc agcacgcaat cgcattatga attggcgaaa ttattcttac aaaatggcat
    11461 ccatgtaaat gtggaaaagc ccatcaccgc aacgatccct caggcggaag agctggtgaa
    11521 tttggctcaa caaaaaaatc tcaaactggc tgtagggcat atcgaaagat tcaatccttc
    11581 gattgtagaa tttaaaaagc atttaaaaaa taccaaaact ctcgagttga ctcgtcttgc
    11641 gacctataaa actcgggggg cggacgtaag tgttcttcat gatcttatga tccatgatat
    11701 ggaccttctc tactggttaa ctggaagcga gattgagagc ttcactgcgg caggttccaa
    11761 acttatttct aaagagttag atacggctca ggtcgctctg aaaatgaaat ctggaacaat
    11821 ggtgtatatt catgtcagcc gcgttttttc aagcattcag cggtggattc gcgcgaccca
    11881 gaaagaaagc attctttacg caaataccgg tactctggaa cttgaaaaaa gtgaagcggc
    11941 ccccatttca ggcgtcggtg gcgacactcc tgtgaaagtg acaaattgga cggtcacgaa
    12001 ggaagacgct ttacaaaaag agacggatgc ctttattgat gctgtactaa ataataagcc
    12061 agtggcggtc agtggattgg atggatggaa gtctttgaag gccattgagg atgtcaaagc
    12121 gaccattgag gctcagttat gaatgaggtc atgattgtcg cggcggaggc ctcgagcgtg
    12181 atctatgctc aacgtctttt agaacactgg caacgtgaag gtaaaaagat caaagccttt
    12241 ggtgtgggca gttccgagat ggaaaaactc ggctttgagc gcattggcaa tgcggaggag
    12301 atggccgttg tgggtgcggc ggaaatcgtg agtgcttata gtcgccttaa gaaagtcttt
    12361 gataatttgg tgattgcggc tgcggcccga aggcctaagg tcgttatcgt catggattac
    12421 ccagagttca atttgatgct cgctaaaaag atgcatgcat tgggcattcc agtggtttat
    12481 tatatttcgc cgcaagtatg ggcttggcgc aaaggccgag ttaaaaccat taagaaatac
    12541 tgtaaaaaag tttttgttct ttttcccttc gaagttcctt tctatgaaga gcatcaagtg
    12601 ccttgcgaat tcgtgggtca tcccattctt gatgagttgg acgacaaact ttttgacgaa
    12661 agctatcgca aaacccaccg caatcaatgc ggaattggcg atcatgaaat ggttttggga
    12721 cttatgcccg gaagtcgaaa tttggaactg aagcagcact tccaaatcca actcgaggtt
    12781 gctcggaaat tgatgaaaaa atatcagaa
//
LOCUS       DATBTE010000330         1684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1411228, whole genome shotgun sequence.
ACCESSION   DATBTE010000330 DATBTE010000000
VERSION     DATBTE010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411228"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VIG33_12470"
     CDS             <1..1073
                     /locus_tag="VIG33_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271696.1"
                     /translation="GKQVYKFKSAEAKFCIDCHKNVHINQFSKNFSAQACTACHTTKN
                     FKERLPFDHNQTQYKLTGAHAKLKCQDCHTPAAPPPINIEWPNTNKKEGPHSLQLAKF
                     LFPEAGKKQCMTCHQDYHMGQLDTRCSECHSDKAWKPTSFNHDLQSNFPLKFKHADVK
                     CSECHKNTNQTVSYKGMQRPVVRFKPVASQCLDCHKDPHKGSLGPRCQECHIEKDWKV
                     TREFHKNFSLTGVHYSLACAECHKDGRKLAGMSQQCYTCHQKDDVHSGTLPDCKECHT
                     QQFWELSRFKHSLTRFPLRGAHRTLDCMECHSNGAYKGLNPQCVVCHKQDYDANPNSQ
                     HNGNLDCAECHRNQFNFSNVRR"
     gene            complement(1048..>1684)
                     /locus_tag="VIG33_12475"
     CDS             complement(1048..>1684)
                     /locus_tag="VIG33_12475"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY8271697.1"
                     /translation="ISKYPKKTNLDKPHCQKSVASYCCVNGMCQLALGMKNILHSKKT
                     ILIISLIFILCGSLTIAKLFLDSSEIKNYNLDLVKSAEAILSNEVKKGTQLVIVNFWA
                     SWCPPCIHETPSMLNYAKNHSDKIQLFLVSEDSSSKEIRDFVKAFSDIYSVNLRIIWD
                     ANRKIGQNFKVYKMPETFIFDNKGKLLRQLSGSVDWSQPELHKLIAEHLKN"
BASE COUNT          532 a          382 c          326 g          444 t
ORIGIN      
        1 cgggaaaaca agtctataaa tttaaatctg ccgaggcaaa attctgtatc gactgccaca
       61 aaaatgttca tatcaatcaa ttcagtaaga acttctcagc tcaggcttgt actgcttgtc
      121 acaccacgaa gaatttcaag gagcgcctgc cttttgacca caatcagact caatataaac
      181 ttactggagc ccatgctaag ctcaagtgtc aggactgcca tacgccagca gcgcctcctc
      241 caataaatat tgagtggcct aatactaata aaaaagaagg cccacatagt ttgcaactgg
      301 cgaagttcct gtttccagag gcgggcaaaa aacaatgtat gacctgccat caagattatc
      361 atatggggca attggacact cgctgctccg agtgccactc ggacaaggcg tggaaaccca
      421 cttcatttaa ccatgacctg caaagtaact ttccattgaa attcaaacat gccgatgtca
      481 aatgttcgga atgccataaa aatacaaatc aaactgtttc ctacaaagga atgcaacgcc
      541 cagtggttcg ctttaagccc gtggcaagcc aatgcctcga ttgccacaaa gacccccata
      601 aaggaagcct gggacctcgt tgccaagaat gccacatcga gaaagactgg aaagtaacca
      661 gagagtttca caaaaacttc agccttaccg gcgttcatta tagcttggct tgcgctgaat
      721 gccacaagga tggacgaaaa ttagccggca tgagccagca atgctatacc tgccatcaaa
      781 aagatgatgt acatagtgga actctgcccg attgcaagga atgccataca cagcaattct
      841 gggaattgag tcgctttaaa cattcattaa cgagatttcc gctacgcgga gcacatcgca
      901 ccttggattg tatggagtgc catagcaacg gagcttataa gggtctgaat cctcaatgcg
      961 ttgtttgtca taaacaagat tacgacgcca acccaaattc tcagcataat ggaaatttag
     1021 attgcgccga atgccaccgc aatcaattta atttttcaaa tgttcggcga taagtttatg
     1081 caactccggc tgtgaccaat cgacagagcc ggagagttgt cgcagaagtt ttcccttatt
     1141 gtcaaaaata aaagtctcgg gcattttata caccttgaaa ttttgcccga tctttcgatt
     1201 ggcatcccaa ataattcgca gattaacgga ataaatatct gagaaagcct ttacaaaatc
     1261 tctgatttcc ttcgaagagc tgtcttcaga aactaaaaaa agttgaattt tatctgaatg
     1321 atttttcgcg taattcaaca tcgagggcgt ttcgtgaatg caaggcggac accaggaagc
     1381 ccaaaaattg acaatcacta attgtgtgcc ctttttaact tcgttagaaa gaattgcctc
     1441 cgcacttttg accagatcca gattatagtt ttttatctca ctagagtcca aaaataactt
     1501 cgcgatcgtt aagctgccgc ataaaataaa aattaaagaa ataatcaaaa ttgttttttt
     1561 actgtgcaag atattcttca tgcccaatgc taactgacac attccattta cgcaacaata
     1621 gctcgcaaca gacttttgac agtgcggctt atcgagattg gtcttttttg gatattttga
     1681 tatt
//
LOCUS       DATBTE010000331         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2299011, whole genome shotgun sequence.
ACCESSION   DATBTE010000331 DATBTE010000000
VERSION     DATBTE010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2299011"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VIG33_12480"
     CDS             <1..226
                     /locus_tag="VIG33_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271698.1"
                     /translation="LWLLKKTTETKQLSVESIVQSYWNSDFSESYFFRLKTATLRFNR
                     ELQIAFNGENLFQLTKQHLELNPHIEVEFS"
     gene            416..1006
                     /locus_tag="VIG33_12485"
     CDS             416..1006
                     /locus_tag="VIG33_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271699.1"
                     /translation="MNKNKCRSQFKKLTFTSLVIAFGWNFYSVASPTEQYPQSRIVDY
                     QTMAGKIEKNNISCAHYSGSWGGNPYSKEIRINTQSLRTPNGVSFPNYMVMSDLWQSE
                     EINCATVNLFLQKSSELISYSATFTIQEYVSMSLVKGQEVCSKYSDKSFTLSFSDPSS
                     GKTYSLFGLGPSQKKLLGTTIVDVASNDFCNLNLFQ"
     gene            1210..>2292
                     /locus_tag="VIG33_12490"
     CDS             1210..>2292
                     /locus_tag="VIG33_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY8271700.1"
                     /translation="MLKIIQIQSPSQVEELFSSFNPQEQSWVVSDLRTKLEMQKLLME
                     RQGYFLDSAVLRASDLWKLLLRRISPELRVVSKDFAHSLLRSFIDKNAEKLNINSTSE
                     KSLFNYMTQLAPLALAADSDEQISKWFADHTDISLRWQSWYLIARAALRWMIYEKGII
                     IVDWIPSYLQEIDGLESVWDEDVIVDLGAEISIVEAEIFQRLSHAQEVLVIEPQPYWQ
                     SEFKYLLQPYEYLRPQAAVIQALNSTRPAPALKESLRLSGMLSEVKNSCQQIRAWLDA
                     KIPATQIAVIAPDIEMYWPILSVYLEQEGIPFQKDLSVKLQSLPSLQQWIATLRARSS
                     RITSADLEVSFYSRQEAQSLRYEEFVA"
BASE COUNT          671 a          441 c          490 g          690 t
ORIGIN      
        1 cctatggctt ttaaaaaaaa caacggaaac aaaacaactg tcagtcgaaa gtatcgttca
       61 gtcctactgg aatagtgatt tcagtgaatc ctattttttt cgattaaaaa cagcaacgct
      121 gagattcaat cgtgaacttc aaattgcttt caacggtgaa aatctttttc aattaaccaa
      181 gcaacattta gaactcaatc cccatattga agtggaattt tcctaacgat tgcggacgtt
      241 gtttcatctt gtaatatttt caggccccga agagttttaa ctcaggtgac ctctcttctg
      301 acgccggggc ttttaggttg ttactatttg tacctggttt tgtcggtcaa gattttctat
      361 aagcaagaca gactttttaa taaaaacaaa taaccttgaa tcaggagtta taaaaatgaa
      421 taaaaacaag tgcagatcac aatttaaaaa attaactttc acgagtttgg taatagcctt
      481 tggctggaac ttctactctg tcgcgtctcc tactgagcag tacccacaaa gtagaattgt
      541 ggattaccaa acaatggctg gtaaaattga aaaaaataat attagctgcg ctcactactc
      601 aggttcttgg ggtggcaatc cttattctaa ggaaatccgt ataaatactc aaagtcttag
      661 gacccctaat ggagtttcct tcccaaatta tatggtcatg tccgatcttt ggcagtcgga
      721 ggagatcaat tgtgctaccg taaatttatt tcttcaaaag agttctgaat taatttcata
      781 ctcagcgaca tttacaatac aagaatacgt tagcatgagc ttagttaagg gccaagaggt
      841 ttgttcgaaa tactcagata agtcatttac tctctcgttc agtgacccta gtagtggtaa
      901 gacctattct cttttcggtt taggcccctc acaaaagaaa ttattgggaa ctacaattgt
      961 tgatgtagct tctaacgatt tttgcaattt aaatctcttt caataacata atcatgctaa
     1021 gacgggcaga attatcgata gaaaagagct tacgctttta actacgacta taaaacgtgg
     1081 attttaatat tacgtatcat ctaaaaagga aaatataaac tgcgtgaaag agacagaatg
     1141 tcaaaggtga tgggtgaaat ttctctgcct ttcatttgat cttcgtgcag gtctcatata
     1201 gtctttgaca tgcttaaaat aattcagatt cagtcgccct cgcaagtgga agaacttttt
     1261 agttctttta atcctcaaga acagtcgtgg gtggtgtccg atttgcggac taaattggaa
     1321 atgcaaaaat tattaatgga gcgtcagggc tattttttgg acagcgcggt cttgcgcgcc
     1381 agtgatttgt ggaaattatt gttacgacgt atatctcctg agctgcgtgt ggtgtctaaa
     1441 gattttgctc attctttgct gcgttccttc atagataaaa atgcggaaaa attaaatatc
     1501 aactccacgt cagagaagag tttatttaat tatatgactc agctggctcc cttggcgctg
     1561 gcggctgact ctgatgaaca aatttccaaa tggtttgcgg atcatacgga tatttctttg
     1621 cgctggcaga gttggtattt gattgccaga gcggccctgc gctggatgat ctatgaaaaa
     1681 ggaataatta ttgttgattg gattcccagc tacttgcaag agatcgatgg gctagaaagc
     1741 gtttgggatg aggatgtcat tgtcgatttg ggagcggaaa tctccatcgt ggaggcggag
     1801 atttttcagc gcctcagtca tgcgcaagaa gtgttggtga ttgagcccca gccttattgg
     1861 caaagtgaat ttaaatattt attacagccc tatgagtatc ttcggcccca ggcggccgtg
     1921 attcaagcct tgaattcgac acgtccggcg ccagcgctga aagaaagctt gcgtctttcg
     1981 ggcatgctaa gcgaagttaa aaacagttgt cagcaaatca gagcctggtt ggatgcgaaa
     2041 atcccagcga cccaaattgc cgtgatagct ccggatattg aaatgtattg gcccatcctg
     2101 agcgtatatc tcgagcaaga agggattcca tttcaaaaag acctcagtgt aaaattgcaa
     2161 agccttccca gcttgcaaca gtggattgcg accctgagag cgcgaagttc tcgaatcacg
     2221 tcagcggatc ttgaagtttc attttattcg cgtcaagaag ctcaaagtct ccgttacgaa
     2281 gagtttgtgg ct
//
LOCUS       DATBTE010000332         2259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_728179, whole genome shotgun sequence.
ACCESSION   DATBTE010000332 DATBTE010000000
VERSION     DATBTE010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728179"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..808)
                     /locus_tag="VIG33_12495"
     CDS             complement(17..808)
                     /locus_tag="VIG33_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEY8271701.1"
                     /translation="MNYKKLHLMGKDLLHQMRDGEIQLVAASLSFSTLLSLVPFLAVI
                     LATFKFLGGDDILYSRVENLLLLYFKEAAGVQFTSIIKTAIRNIHAGGLGVIGASFLV
                     LTSFRLMHDMEYGIHRVWNQRNTRPLLKRFFIYWLLVIILPVFLAIYVGFITLIRMDF
                     GKQYLPGQITGMAVLILVLHLVYRYVPNIKVKAAPAFFSAVVAAIGLFVVQNTFTWVT
                     VKFFRMSRIYGSFAALPIFLVWILTIWYVILGGVAICASTQRRQL"
     gene            complement(810..1718)
                     /locus_tag="VIG33_12500"
     CDS             complement(810..1718)
                     /locus_tag="VIG33_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8271702.1"
                     /translation="MHTPDQKPNEKLHLKIGPYEICPIPTGGFGLDGGAMFGTVPKVL
                     WEKSHPADDKNRIQMEARGLLLKSPNNNILIDCGNGGDFIAKYGDKLGSKFVEMYNIN
                     NQGPSLQNSLKAYGLKAEDIHHVILTHLHFDHAGGATTERDHKLVPTFPKAKYYVQKA
                     NLDTASHPNLRERASYFPANYQPLLEAGVLTVLEGPRENLLPGISTLISNGHTQGQQM
                     IKITDDSTTLLYCGDVVPTSSHVKVPWLMGYDLHPLVLMEEKQKYLSEAADHSWYLFF
                     EHDPYCDSARIEKKGADFAVKERYWL"
     gene            complement(1690..>2259)
                     /locus_tag="VIG33_12505"
     CDS             complement(1690..>2259)
                     /locus_tag="VIG33_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8271703.1"
                     /translation="YPKLLVISNHTSHLDAVSVAAAIPRRYWLDLYIAAAKDYFFTNP
                     VFTFFSKHCLGAIPIDRKDHRGEAINLILRLLSTLPRIWLIIFPEGTRSKDGKIQEFK
                     RGISIFSERTKTPILFLYIDGNANLWPKGNLFPKPGKITIHVGPVHPPGDIKTVFDAY
                     KAWVMTINPDSFSSTQQGEPNAHSGSKTE"
BASE COUNT          608 a          548 c          545 g          558 t
ORIGIN      
        1 aagtcaagtc attctcttac agttgtcgcc tttgggtgga ggcgcagatc gcgacacctc
       61 ccaaaattac gtaccaaatg gtgaggatcc aaaccaggaa aatgggcagg gccgcgaagc
      121 ttccgtagat ccggctcatg cggaaaaatt tcacggtcac ccaggtaaag gtgttttgca
      181 ccacaaataa gccaatggcc gctacaacgg ccgagaaaaa ggcgggggcg gccttcacct
      241 tgatgttggg tacataccta tagaccaaat gcaggaccaa tataagcacg gccatccccg
      301 ttatttggcc cggcaaatac tgcttgccaa aatccattcg gatcagggtt atgaagccta
      361 cataaattgc taaaaaaaca ggcaatatga tcacgagcag ccaatagatg aaaaagcgct
      421 ttaacaaagg acgggtgttt ctttgattcc acacgcgatg gattccatac tccatatcgt
      481 gcatcaggcg gaagctggtg aggaccaaaa aactcgcacc tataacaccc aggcccccgg
      541 cgtggatgtt gcgaatcgcc gttttgatga tagaggtgaa ttggactccg gcggcttctt
      601 tgaaataaag cagcagcaga ttttccactc gtgagtacaa aatatcatcg ccccctaaaa
      661 atttaaaggt agcaaggatc acggctaaga agggaaccaa agataacagc gtcgagaaag
      721 acagggaggc tgccaccaac tggatttctc catcgcgcat ttgatgaaga agatccttgc
      781 ccatgagatg aagcttctta tagttcattc tataaccagt agcgctcctt gactgcaaag
      841 tcagcgccct ttttttcaat gcgtgcagaa tcacagtacg gatcgtgctc aaaaaataaa
      901 taccacgagt gatccgccgc ctctgaaaga tatttctgct tttcctccat caagaccaag
      961 ggatgcaagt cataacccat aagccacggc acttttacgt ggctgctagt tgggaccaca
     1021 tctccgcaat acaacaaggt cgtcgaatca tccgtgatct tgatcatctg ctggccctga
     1081 gtatgaccat tcgagatcag tgtggagatt cctggcaata aattttcccg tggtccctcc
     1141 aagaccgtca aaactccggc ctctaagagc ggctgataat tcgcgggaaa gtaactcgcc
     1201 cgttctcgca ggttgggatg gcttgccgta tcgagattgg ctttttgcac atagtacttg
     1261 gctttcggaa atgtggggac cagcttgtgg tctctttccg tcgttgcgcc accggcatga
     1321 tcgaaatgca aatgagtcaa gattacgtga tggatatcct ccgccttcaa accataggcc
     1381 tttaaagagt tctgaaggct cgggccctga ttatttatat tgtacatctc gacaaacttg
     1441 ctgccgagct tgtcgccgta tttggcgatg aagtctccgc cgtttccgca atcgatcaga
     1501 atattattat tcggggattt cagcagcagc ccccgtgctt ccatttgaat gcggttttta
     1561 tcatcggcgg gatggctctt ttcccacagg accttcggga ctgtgccgaa catcgctccg
     1621 ccatccaggc cgaagcctcc ggttggaatg gggcagattt cgtagggacc tatttttaga
     1681 tgtaatttct cattcggttt ttgatccgga gtgtgcattg ggctccccct gttgagtaga
     1741 agaaaaagag tctggattga tcgtcatcac ccaggctttg taagcgtcaa agacagtttt
     1801 gatatcgccc ggaggatgaa ccggtcccac atgtatcgtg atcttaccag gttttggaaa
     1861 caaatttccc ttcggccata aatttgcatt gccatcaatg tataaaaaca gaataggggt
     1921 ttttgtgcgc tcggaaaaaa tactgatgcc acgcttaaat tcctgaatct ttccgtcctt
     1981 ggagcgggtg ccctcaggaa agataatcag ccaaattcgc ggcaaagtgc ttaacagcct
     2041 caggattaaa ttgatcgctt ctccgcgatg atccttacgg tcgatgggga tggcgcctaa
     2101 acaatgcttg gagaaaaaag taaagaccgg attcgtgaaa aaataatcct tagccgcagc
     2161 aatgtagaga tccaaccaat atcgtcgtgg aatggccgcc gccaccgaaa cggcatccaa
     2221 atggctggtg tgattggaaa tgaccaggag cttcggata
//
LOCUS       DATBTE010000333        12927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_113396, whole genome shotgun sequence.
ACCESSION   DATBTE010000333 DATBTE010000000
VERSION     DATBTE010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 12927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12927
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113396"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..2188
                     /locus_tag="VIG33_12510"
     CDS             185..2188
                     /locus_tag="VIG33_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271704.1"
                     /translation="MKKLMIVFSVILLLTQLVSPMASGAPLDGDLKNGIEGIWSLQGT
                     SSSLGPYSGKLELRKADGSYNAIRIITYLNKSFEGFKIQEVWTGQARMEEKTLTVRYN
                     LPQGGFITQLNGKKRDELDFKTFARIEERFRPSEKGLSARFTDKNNSIYIELLTSPQK
                     LEILPLSIPLWRDRRIHLDAKGPRIPWSIRGVIDLFKRKIGYEKDAVVKSYRNRKEFL
                     EERPSIVFDPTDFDFYRQNKDMIRVINKITDDISATEALTKRNAYAPSLAEKQQGYER
                     NTVTHHINEQGMVSTASVDKQGHLLRYEADGDSALWTGMYVGSQAMRYLVAHEAAALE
                     NVKRSLKGLFILMDITGDSKEFARTLVTYTPGKALPAKWHRGRGRFQEIMWLEGGNND
                     MVKGIAHAFLWATLVVPAEERAIWLHLKEKSLRLLKLRAIEERIQNRIMALGLAALLN
                     QDSKLHEEYANSFSNPTVKITGYSFDYSYYWHGTADWSGVNLGVVGDLTKIMIADRLG
                     ERKIADQLRERLMDSWVVYAPARRSLVTLAAYGFAKHHSSEEIFHSALTQAVWGLREI
                     PYPRPNLEMEYDHSLNPAWCISPLPRMFWKAANSPMPSIDFFYQGLYGYPVFEAGAFS
                     SNFIWTDGAFQYKGRHNKGVEFAGVDYLFAYWLARYSGVPNLN"
     gene            2258..2515
                     /locus_tag="VIG33_12515"
     CDS             2258..2515
                     /locus_tag="VIG33_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271705.1"
                     /translation="MRNRVLPTEKGVSGEAGHKDILFYIPLSKPIDYIEGFEEAVTEA
                     LKKSSSAVALTDVKVKRSILITFLYNEWCIVIEGAPVSKVP"
     gene            2923..3462
                     /locus_tag="VIG33_12520"
     CDS             2923..3462
                     /locus_tag="VIG33_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271706.1"
                     /translation="MFFSRVNTNFIAAVVLAFVSPLVTPVNGFAYSSVQEINEFLAKN
                     ECKIKAMNVADNTEKYQRNLNWEIASENSSFFLKKTRVEYFRGHRSHIFVPAPSPEVG
                     VPYRELGVDEEFYIYFDLNNPTDEGASVSDLFIYTNNTFTEVPAKLENFATNEPFSLK
                     FRTQMNGDRYYFFQCSPKN"
     gene            3638..4756
                     /locus_tag="VIG33_12525"
     CDS             3638..4756
                     /locus_tag="VIG33_12525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY8271707.1"
                     /translation="MAYMQTLHKIIGEIVASSAAAVDQNASFPRASMEAFGKTGFLGL
                     ISAKEGGGMGLGLGDAARVIEALAQVCPSTAMVLTMHYCGTAVLEKQGSLEIRKEIAK
                     GNHFTTLAWSEVGSRSHFWAPVGTARKVQGGYSVSCQKTMVTSAMEADSYVWSSQATS
                     GNGASTLWLVQKNTSGLKSSQAFNGLGLRGNASAPMASENMVLPESAMLGNDGGGFDT
                     MIGVVLPLFSVLNASCSLGIIEGSLAMSLQHVTGNKFEHLNSALSDLPTIRAYVAKAR
                     IRADMVRTLRDDTVVALKENRPDAMLRVMQVKAAAADAALDVTDICMRVCGGAAFRKD
                     VGMERLFRDARAAHIMAPTSDVLFDFIGKAICNMPVFG"
     gene            4767..5624
                     /locus_tag="VIG33_12530"
     CDS             4767..5624
                     /locus_tag="VIG33_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEY8271708.1"
                     /translation="MSRRFTLGAVAYAPKVITIWEGFKDYFSQHRFDFDYILYSNYEA
                     QVEAMFEGAIDAAWNSPLAWIRAERMGRARGTPVSFGPMRDTDQDLTSVLVVRKEAGI
                     SSLQDLRAKNIGFGAVDSPQARLIPLIHLRQSGLVADKDYRARVFDVLGGKHGDHIGG
                     ERDAAIALMKREVDATWMIDGNYQAFSQEGTLPSGETVVLARTENYDHCIFTLAPGIA
                     KESVNQFSEILLSMKWSDSKVRPLLELEGLKEWRPGRTKGFTLLNQAVESEHFYDSKG
                     QIIEPNYRY"
     gene            5602..6822
                     /locus_tag="VIG33_12535"
     CDS             5602..6822
                     /locus_tag="VIG33_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY8271709.1"
                     /translation="MSPIIDIENLGFDMGAHLLVKRGLAAIPVGSELLVKGSAANWQV
                     QLSAWCREQGHSVRFCREGEQTEQTRHEVALVCRGSAESERWTQAEKTGFSDVKKEGA
                     VAEEAQPNWGLAARGATTEAGGPAFHFRLNKKTDIWASNASELYAHAVAAQWDPEKAI
                     VWSEPRQHSQEMEDAVIQVMTYMIENENAALLVPARFLGQLHPHFREVQALLAIQVAD
                     EARHIEVFTRRIRLYGGEPAVSTAGGQASLKTLLDEPDFSVAGFLLSILGEGTFVDLL
                     QFMHQYAPDAVTRQMCLLAARDEARHVSFGMSHLREHLEKDPMLQGRLRTATEARFEG
                     LAKTSGLNAEVFDSLILIAAGECTPAAIAQGFQRVQNLLREMENGRRSRLEKLGFTAQ
                     ESQRLASLHTRNFM"
     gene            complement(6851..7810)
                     /locus_tag="VIG33_12540"
     CDS             complement(6851..7810)
                     /locus_tag="VIG33_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY8271710.1"
                     /translation="MKKYWSHLIIYIIASGLIPVVYNGCGKLNGFNLIDSGTDIGSTG
                     ARTPPENGNLGEDVLVADQLALGQALYEKNCTICHGTLALSTKLGKTSADITTAFKTV
                     PSMASLASLTTEQVAQIALALNKNSLSLKNETSVHYRTLISNRYLLSSQLHELFVAST
                     NLDSNDTTIQNTINSLVMKHPEGFGGNCSRNDPGCLPNPCANNGACRGQLSTSQNAEV
                     NPSSSPVRKGYLIQACEKILSVDKAVNNLAQKAAIDVTQAPTLATLKTLALFAHRERP
                     QDSAALDQLMSLANSAKTANMNVLDQWRFVMLALCEASTSDLL"
     gene            complement(7807..9435)
                     /locus_tag="VIG33_12545"
     CDS             complement(7807..9435)
                     /locus_tag="VIG33_12545"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY8271711.1"
                     /translation="MGHKNSCHKKNLSRRDFLSFMGMSTGLPLLQTPLQILIESIVLG
                     AHQKAMAQSSGLKPRKYLHILQEGAPPRWNFDLFLTPYSSAGFVANAQLGTTYVKSGS
                     STSLAYTTTVKKNINVPLLWQFNVPAANGGTRSMDPLLDNLLQLRGIEVGNPDHGAAQ
                     QLQFVPLGAAQSMSALAADISSAPIPGINLSLEQYQFKSQASKSAITVPNGSNMISTL
                     LTPFVRNNSSSFETKRKTLGAALDSAISLLNQAATTQNAGAVSLATSTQAASDLLSKG
                     FGDLDSIWTNLFNKYNSLVTRSLDPMQSLPGINDQAIASDGTTKFQINGNIVSSPDIR
                     TMMTTSCKVTRLAAHFAVAEYVLLNNLSDSISIGPGGMEGLNINNSSARLGTDEHETS
                     GTISLMVNSFLNLAYAACLLELMDQLKAKNIFNETVIVMGGEFGRSPRKDATGSDHGY
                     LGSSSSIYCGALAGPMILGNIYNLKGQSDADAGTWGRGAPVTQLGRALNQSDWASTVA
                     FLLRSPSPTTAATSLLAANPDGSISSLIEKARQI"
     gene            complement(9425..10888)
                     /locus_tag="VIG33_12550"
     CDS             complement(9425..10888)
                     /locus_tag="VIG33_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271712.1"
                     /translation="MGSFVERTALSKAVIFSFIFTPFLFGQIVQAEDLSEVALFHRCY
                     SQLTSQRVATTDPLFQQVKSGKLTAIDACNQVLDSAKLSGSSAMTLANPNDVRAQNVL
                     ATFHRLHASWFYAKDFPVISWPGHSDDMKDLYDSSSPALYYTRALFGNGISASDPVMT
                     PDFLQAIRTNMSPSAGPESNHSTSDYIFQTPFKFAATGTLLGIQIAPSTVLNFPAFKN
                     HPAGSIDLYSNRGGGFLGTMTYLNLNISSISSYSPYKTDGAVQMHRRWGKAVYRDTLC
                     RDLPVVREADVAALVQTSSPVSFRTSNSCVKCHASHDRISGVVRGMNILYVGTGDPTG
                     NGTAERGGNFSQFYPVTKSAEAAWPASPDADYYQRPPNGQVYFRNYNGDLIDQSVTGV
                     NDLGAKLAATDDYYICLAKRYYNYFLGIDVDTGDLNDPAHPVALSPAALIHRNLVINL
                     GKSVKSSQNISQLIKDILKLSNYRKVDIGISGATNGP"
     gene            complement(10970..12610)
                     /locus_tag="VIG33_12555"
     CDS             complement(10970..12610)
                     /locus_tag="VIG33_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271713.1"
                     /translation="MNKAKSGFFKKNKKRVVVGLATILWTTINPFNFLNQSFAESNVS
                     SSNVSFLKKVVQDLNIQVSLSSVAGIKGSNPELDVTQQNLAVKISNQDAELMDVLRKN
                     LGTKQGYSLQEGFIMQVRLAGTVSPLIVGGKTRKDAIKAVDAEVEEGAAQKREALYQM
                     APKMVEDGLRAKITAAVNSNPNLNMLTAGQKAQLIDGQVKQNLPAALQTSLPLVHKQI
                     DAQVAQAVPGIKAAMEEDHVNSRMSQLELETGIQTLDATIGIPINPLGNMSDTPPGLI
                     LFSFGKAHPMLGAELKANGLSQWENILGHASEAERGNTVSATGYSSIGIAYSLTKDIN
                     LMVNIIGYHDRDPWMSAQQYIQQSLAVSKAVFNKYQDVTALNALALSMRADAKLNKSL
                     TGQFWATITNRFDPTQGDQATGYSFGGTITNEKSKTMLNFAYAHSGKAYFNETYSIGI
                     AQTILDKKTNSWVPGVIVFTDFRGIKQLANVNVLGSVASETKLVALGSKVDICKYVDQ
                     RLKIDCALVGEYQKLVQQSGGEHPSDDFYVGLSANAAY"
BASE COUNT         3425 a         2819 c         3145 g         3538 t
ORIGIN      
        1 tctcactctc aactgaaggt gaaaataaat aaaggccttt cctaaggttt cttttccaca
       61 tttgccccgc aatttttaga aagatcccgg actcttgggg tcatctcttg tttcgaacct
      121 tcatcaaaat cttcttaaaa tctgaaagtg atttagtgtc ataaatgagg tttgcttaaa
      181 aaccgtgaaa aaactcatga ttgtcttcag tgtaattttg cttctcactc aattggtcag
      241 tccaatggcc tcgggagccc ctcttgacgg cgacttgaaa aatggcattg aaggaatctg
      301 gtctcttcaa gggacgagtt cttcgctggg accctatagc gggaaattgg aattgcgcaa
      361 agcggatggc tcgtataatg ccattcgtat catcacctat ctcaataaat cctttgaggg
      421 ctttaaaatc caggaggtct ggaccggaca ggcgcgaatg gaggagaaga ctctgactgt
      481 tcgatataat ttaccccaag gtgggttcat cacgcagctc aatggaaaaa aacgggacga
      541 attggatttc aaaacctttg ctcgcattga agagcgcttt cgaccttccg aaaaaggctt
      601 gagcgcgcgg tttaccgata aaaataattc tatctatata gaactcctta cgagccctca
      661 aaagttagag atccttccgc tttccattcc gctttggcgt gacaggcgca tccatctgga
      721 tgccaagggg ccgcggatcc cttggtccat tcgtggagtc atcgacctct ttaaaaggaa
      781 aatcggttat gaaaaggatg ctgtcgtaaa gtcctataga aatcgcaaag aattcctgga
      841 agagagacct tcgatcgtct ttgatcccac ggactttgat ttttatcgcc aaaacaaaga
      901 catgattcga gtgattaata aaattaccga tgacatcagc gcgacggaag ctctgaccaa
      961 acgcaatgcc tacgccccca gtcttgcgga aaaacagcag ggatacgagc gcaataccgt
     1021 gacccatcat attaatgagc agggcatggt ttcaacggca agtgtggata agcaaggaca
     1081 tttgctccgc tacgaagcgg atggggactc agccttgtgg acaggaatgt atgtaggatc
     1141 tcaggccatg cgctatctag tcgctcacga ggctgcggcg ttggaaaatg taaaaagatc
     1201 gctgaagggt ctttttattt tgatggacat cactggcgat agcaaagagt tcgcaagaac
     1261 tctggtcact tatactcctg ggaaagcgct gccagcaaag tggcatcgag gcaggggacg
     1321 cttccaagag attatgtggc tcgaaggcgg aaacaacgat atggtcaaag gcatcgctca
     1381 cgccttttta tgggccaccc tggttgttcc cgcagaggaa agagcgattt ggcttcatct
     1441 caaagaaaaa tcccttcgtc tgctcaagct cagggcgata gaggaaagaa ttcagaatcg
     1501 catcatggcc ttgggccttg ccgccctcct taatcaagac tcaaagcttc atgaagagta
     1561 tgcaaatagt ttttcaaatc cgaccgtaaa aattacggga tattcctttg actacagtta
     1621 ttattggcac ggaaccgcgg actggagcgg agtcaatctg ggggtcgtag gcgatctcac
     1681 caagattatg atcgcggacc gtctgggaga gcgaaaaatt gccgatcagc tgcgtgaacg
     1741 tcttatggat tcctgggtgg tttatgcccc cgctcggaga tcccttgtca ccttagcggc
     1801 ttacggattt gccaaacacc acagtagcga agagattttt cactcggcgc tgacacaagc
     1861 cgtatggggt ctccgtgaga ttccctatcc tcgtcccaat ttggagatgg aatacgatca
     1921 ctctttaaat ccggcgtggt gtatttctcc tcttccgcgc atgttttgga aggcagcgaa
     1981 tagtcccatg ccatccattg atttctttta tcaagggctt tatggttacc cagtattcga
     2041 ggcaggcgcc tttagcagca actttatttg gacagatgga gcctttcagt acaagggtcg
     2101 ccacaacaaa ggagttgagt tcgcaggagt tgattatctt tttgcttact ggctcgcacg
     2161 ctattcagga gtgccaaatt tgaattaaaa aaatggcatt atcctgaaac agagcgaatt
     2221 taagagatat tttaaccaga gtcgtcgcca atctattgtg agaaacagag ttcttccgac
     2281 ggagaaggga gtctctggcg aagcaggcca taaggatatt ttattttata ttcctctctc
     2341 aaaacccata gattatattg aaggcttcga agaagcagtt acggaggctt taaaaaaatc
     2401 ttctagtgcg gtggctctca cagatgttaa ggtaaaacgc tctattttga taacgtttct
     2461 ttataatgag tggtgtattg tcattgaagg agctcccgtt tctaaagtcc cataaataaa
     2521 attaatcctg cctttaaatc agcccgaact aaagtgcgcg ttcccgatca tggggagcaa
     2581 agagtccttt acatagctta agccgagcag cgtattatgc aactcagtag cgtaatacgc
     2641 gattgagtta aaaggtcgtg caacacctcc ctattatagg cattcctttt ctaaaaaaat
     2701 aaatcaactt gtccatgtta tttcttgtaa tcaaacagaa cttgatacta ttttcatgac
     2761 aagatcagaa taaacagaag attatgttga agcgatgagt gctctactaa gaggaatgaa
     2821 tgggcagctt tcgtcccaag aaattcggag aaggttccgc caaactttcg aacgatatta
     2881 attttagaag gatctgatcg attcacttaa ataggaggta ctatgttttt ttcaagagtt
     2941 aataccaatt ttattgcggc cgttgtcttg gcgtttgtgt cgcccctcgt gacacccgtc
     3001 aatggctttg cttattcctc agttcaggaa ataaatgaat tcttggctaa gaatgagtgc
     3061 aaaatcaaag cgatgaacgt tgctgataac acggagaaat atcagagaaa tttaaactgg
     3121 gaaatcgcaa gcgaaaactc atcgttcttt cttaaaaaaa cccgcgttga atattttcgt
     3181 ggccaccggt ctcatatttt tgttccggcc ccttcgccgg aagtgggagt tccttaccga
     3241 gagctagggg ttgatgagga gttttatatt tactttgatt tgaataatcc gacggatgag
     3301 ggggcatcgg tatctgattt atttatctat accaacaaca cttttactga ggttcccgca
     3361 aaacttgaaa atttcgccac caatgaacca ttttctttaa agttccgaac tcaaatgaat
     3421 ggggatcgct attatttctt tcagtgcagt ccaaagaatt aaatccttag aatattcaat
     3481 attaaaaaca aaataactta aattaaaata tataaaaata tataaaaatg ctttgaaggt
     3541 cgggcatcaa acgtgagttt agatgccatt tttgcttttg tctgatagaa ataaatctcc
     3601 atattataaa tcccttactc caaccaaagg atcccccatg gcttatatgc aaacccttca
     3661 taaaatcatt ggcgaaattg tggcttcttc agccgcagca gtagatcaga acgcaagctt
     3721 ccctcgggct tcgatggagg cttttggtaa gaccggcttt ttgggtttga tcagcgcgaa
     3781 agaagggggc ggaatggggc tcggtcttgg ggatgcggcc cgggttattg aggcgctggc
     3841 tcaggtttgt ccttcaacgg cgatggtttt gaccatgcat tattgcggaa ctgcggtcct
     3901 tgaaaagcaa gggtctcttg agatccgtaa ggaaatcgct aaaggcaatc acttcacaac
     3961 cttggcttgg tccgaagtgg gttcgcgcag tcatttctgg gcgccggtgg ggaccgctcg
     4021 caaagttcag ggtggatata gcgtgagctg ccaaaagacc atggtgactt ccgcaatgga
     4081 agcagactct tatgtttgga gttcccaggc cacgtcaggc aatggagcct ccaccctgtg
     4141 gttggtgcaa aaaaacactt caggattgaa aagctcccag gcctttaatg gtttgggact
     4201 tcgtggaaat gcctcggccc cgatggccag cgaaaatatg gtattgcccg agtccgcaat
     4261 gctcggaaat gatggcggtg gttttgatac catgattggt gtggtattgc cgcttttcag
     4321 tgttctcaat gcttcttgtt ctttggggat tatagaaggc agtttagcga tgtcccttca
     4381 acatgtgact ggcaataaat ttgaacatct caattcggcc ctctcagact tgccaaccat
     4441 tcgcgcttat gtggcgaaag ctcgcatccg tgcggacatg gtgcgcactc tgcgtgatga
     4501 taccgttgtg gctctgaaag agaatcgccc ggatgcgatg cttagagtga tgcaggtgaa
     4561 agctgccgca gcagatgcgg ctttggacgt caccgatatc tgtatgcgtg tgtgtggcgg
     4621 cgccgccttc cgcaaagacg tgggaatgga gcgtcttttc cgtgatgcgc gggcagcaca
     4681 tattatggcg ccgacttccg atgtgctttt tgatttcata ggcaaggcta tttgcaatat
     4741 gccggttttc ggttaaggag aattttatga gtcgtcgttt tactctcggc gctgtcgctt
     4801 atgcacccaa agtgatcacc atttgggaag gttttaaaga ttacttttcg caacacagat
     4861 ttgattttga ttatattctt tattcaaatt atgaagccca ggtggaggca atgtttgagg
     4921 gtgccattga tgcggcctgg aattctcctt tagcttggat ccgagcggag cgtatggggc
     4981 gagctcgggg gacgccggtc agctttggtc ccatgcgcga caccgatcaa gatttgactt
     5041 ccgttttagt cgtgcgaaaa gaggcgggga tttctagctt gcaagacctc cgtgcgaaaa
     5101 atattggctt tggagccgtg gactctcctc aggcccgctt gattcctctg attcatctca
     5161 ggcaatcagg gcttgttgct gataaagact atcgagcacg agtctttgac gttcttgggg
     5221 gcaaacatgg ggatcacatc ggaggtgagc gagacgccgc gattgcttta atgaaaagag
     5281 aagtggatgc cacctggatg atcgacggga actatcaggc tttttcccaa gaagggactt
     5341 tgccttccgg cgaaactgtg gttttggccc gcacagaaaa ttatgatcat tgtattttca
     5401 ccctggctcc cggaattgca aaagagagcg taaatcaatt tagcgaaatt ttattgtcga
     5461 tgaagtggag cgattccaaa gttcgcccct tgcttgagct ggaaggctta aaagaatggc
     5521 gaccaggccg aaccaaagga ttcactttgt tgaaccaagc ggttgagagc gagcattttt
     5581 atgattcaaa aggacaaatc attgagccca attatcgata ttgaaaacct tggctttgat
     5641 atgggggccc atctcttggt taagaggggt cttgccgcta ttccggtggg gagcgagctt
     5701 ttagtgaaag gttcggccgc aaattggcaa gtgcagttgt cggcctggtg ccgagagcaa
     5761 ggacattcag tccgtttttg tcgagaaggc gagcagactg agcagaccag gcatgaagtg
     5821 gctttggttt gtcgtggctc cgctgaaagc gagcgttgga ctcaggctga aaaaaccggt
     5881 ttcagtgatg tgaaaaaaga aggtgcggtg gcggaagagg ctcagcccaa ctggggactt
     5941 gcggcgcgag gagcgaccac tgaagccgga ggcccggcct tccattttcg tctcaataaa
     6001 aaaaccgaca tttgggccag caatgcttct gaactttatg ctcatgcagt ggccgctcag
     6061 tgggatcccg aaaaagccat tgtgtggtct gaaccccgtc aacatagtca ggagatggaa
     6121 gacgccgtta ttcaagttat gacttatatg attgagaacg agaatgccgc gcttttagtg
     6181 ccagcaagat ttttggggca gctccatccg cattttcgcg aggttcaagc gctgttggct
     6241 attcaagtgg ccgatgaagc ccgccatatc gaagtgttca cccgtcgtat ccgtctttac
     6301 ggaggagagc ccgccgtgtc gacagcggga gggcaggctt cgctaaaaac tcttttagat
     6361 gaacccgatt tttccgtcgc aggatttctg ttgtccattc taggagaagg cacttttgtg
     6421 gatctcttgc aatttatgca tcagtatgct ccggacgcgg tgacccgtca gatgtgtctt
     6481 cttgcggctc gggatgaagc tcgccatgtt tccttcggca tgagtcatct gcgtgaacat
     6541 ctggagaaag atcccatgct ccagggacgt ttgcgcactg ccactgaagc cagatttgaa
     6601 ggactggcca aaaccagtgg tttaaatgcg gaagtttttg attctttgat tttgattgcc
     6661 gccggggaat gcactcctgc agccattgct caaggttttc agcgggtgca aaatttattg
     6721 cgtgagatgg aaaatggtcg caggtctcgc ttagaaaaat taggttttac cgcgcaggaa
     6781 tctcaacgtc tggcgagtct gcatacgcga aatttcatgt aagcagcact gtaccaagtc
     6841 ctcagagcgc tcaaagtaaa tctgaagttg aggcttcaca cagagccaac atgacaaatc
     6901 tccattgatc gagaacattc atattcgctg ttttcgccga attggcaagg ctcatcagtt
     6961 gatccagggc cgcactgtct tgaggacgtt cgcgatgcgc aaacagggct aaggttttca
     7021 gcgtggctaa agtgggagcc tgagtgacat caatggcggc cttttgcgcc aaattgttca
     7081 ccgccttgtc tacactgaga attttttcgc aggcctggat cagataaccc ttgcgaaccg
     7141 gactgctgga aggatttact tcagcatttt ggctggtgct caattgaccg cgacaggctc
     7201 cattgtttgc acaagggttg gggagacaac caggatcatt tcgtgagcag tttccaccga
     7261 aaccttcggg gtgtttcata accaaggagt tgatcgtgtt ttgaatggtg gtgtcgttac
     7321 tatccagatt tgttgaggcg acaaacaatt catgtaattg agaactcagt aaataacgat
     7381 tgctgatcaa agtccgataa tggacgctcg tttcattttt aagagagagg gagtttttat
     7441 tcagagccaa tgcgatttgc gccacttgtt cagtggttaa agaagccagt gaggccatgg
     7501 aagggaccgt tttaaaagca gtggtgatat ccgcagatgt ttttcctaac ttagtagaga
     7561 gcgccagggt gccgtggcag atagtgcagt ttttttcata gagcgcttgc cctaaagcca
     7621 gttggtcggc tactaaaaca tcctcaccta agtttccgtt ttcaggaggg gttcgagcac
     7681 cagtgcttcc aatatcggtt ccggaatcaa tgagattgaa accgttgagt ttaccgcaac
     7741 cattatacac cacgggaata aggccactcg caataatata aattataaga tgactccaat
     7801 acttcttcat atctgcctgg ccttttcaat aagagaactg atagatccat cagggtttgc
     7861 tgcaagcaaa gaggtcgcag cggttgtcgg cgagggagat cgcaagagga aggccaccgt
     7921 cgaagcccaa tcgctttgat tcagcgcacg gcctaactga gtgaccggag ctcctcgtcc
     7981 ccaggtgccg gcatcagcat cgctttgacc tttgagatta tagatgtttc ccagaatcat
     8041 agggcccgcc aaagctccgc aatagatgga agagcttgag cccaagtacc catgatccga
     8101 tcccgtggca tctttacgag ggcttcgacc aaattctcca cccatgacaa tcacggtttc
     8161 attaaaaata ttttttgcct ttaactgatc catcagttcc agcaagcaag ccgcataggc
     8221 aagattcaaa aaagaattaa ccatcaatga aatggtgcca ctggtttcat gctcatcggt
     8281 tcctaatcta gcactcgaat tattgatatt gagcccttcc attccaccgg gaccaataga
     8341 aatagagtcg cttaagttat tgagcaatac atactcagcc accgcgaagt gggctgccaa
     8401 tcgagtgact ttacaggaag tggtcatcat agtacggatg tccgggctgg aaacgatatt
     8461 tccattaatt tgaaactttg tggttccatc cgaagcaatg gcctgatcgt ttattccggg
     8521 aagagattgc atgggatcaa ggctgcgggt cactagactg ttgtatttgt taaagagatt
     8581 tgtccagata gaatcaagat caccgaaacc tttgctcaat aaatcactcg cagcctgggt
     8641 ggaagttgcc aagctgacgg ctcccgcatt ctgagttgtg gccgcttgat tcagtagact
     8701 gatggcggaa tccaatgcgg cgccaagagt ttttcttttt gtttcaaagc tagaagaatt
     8761 atttcttaca aaaggagtca gcaaagtgga aatcatgttt gagccgttgg gcacggtgat
     8821 agcggatttc gaagcttgcg atttaaactg atattgttct aaggataagt taattcccgg
     8881 aatcggagca cttgaaatat ccgcagcaag agcgctcata ctctgcgcag cacccagagg
     8941 tacgaattgc agttgttgag ccgcgccgtg atcggggttt cccacttcaa ttccgcgcaa
     9001 ttgaagtaaa ttgtctagaa gaggatccat gctacgggtt ccgccattcg ccgctggcac
     9061 attgaattgc cataatagcg gtacgttgat gtttttcttt accgtcgtag tataagccaa
     9121 acttgtggag gagccagatt taacgtaagt tgtgccgagt tgggcattgg cgacaaagcc
     9181 tgcggaagag tacggagtta agaataagtc gaagttccat cgaggaggag cgccctcttg
     9241 aagaatgtgc aaatattttc gaggttttaa tcccgagctt tgagccattg ccttttggtg
     9301 ggcaccaagg acgatggatt caatcaaaat ttgcagagga gtctggagca aagggaggcc
     9361 agtggacatc cccataaagc ttaaaaaatc acgtcgactt aagttttttt tgtgacaact
     9421 atttttatgg cccatttgtt gccccgctga ttccaatatc tactttgcga taattagaaa
     9481 gcttaagaat atctttgatc aactgagaaa tattttgaga actcttcaca gacttcccta
     9541 ggttgataac caggttgcga tgaatgaggg ccgcagggct gagcgccact ggatgggcag
     9601 gatcgttcag gtcacccgtg tccacatcaa tgcccaaaaa gtaattataa tatcttttgg
     9661 ctaaacagat atagtaatca tctgtcgcgg ctaattttgc gcccaagtca ttcactcccg
     9721 taacggattg atcaatcaag tctccattgt agttgcggaa gtaaacctgg ccgttagggg
     9781 gcctctgata ataatccgca tcgggactcg cgggccatgc agcttcagca gattttgtta
     9841 cggggtaaaa ttgactaaaa tttcctccgc gctcagccgt gccattccca gtgggatcgc
     9901 cagtccccac ataaaggata ttcattccgc gaaccacacc agaaattcgg tcgtgtgaag
     9961 catggcattt tacgcaggaa ttgctggttc taaaagaaac gggactggaa gtttgcacca
    10021 acgcagcgac gtccgcttca cgcaccactg gcaggtcacg gcaaagggtg tcgcgataaa
    10081 ccgccttacc ccagcgacga tgcatttgca cagctccgtc ggttttgtag gggctatatg
    10141 agcttatact tgaaatattg agattcaaat aagtcatggt tcccaaaaac ccaccgccac
    10201 gattggaata caaatcaatt gatccggcag gatgattttt aaaagccgga aaattaagaa
    10261 ccgtgctcgg agcgatttgg atgcctaaga gggttccggt agcggcaaac ttgaacggtg
    10321 tctggaatat ataatcactg gtggaatgat tggattccgg tccggccgag ggactcatgt
    10381 ttgtacgaat ggcttgtaaa aaatctgggg tcatcaccgg gtctgaagcg gaaattccat
    10441 ttccaaaaag cgcccgcgta taatagagag ccggagagct tgaatcgtac aggtccttca
    10501 tatcatccga atgaccaggc cagctgatca ccggaaagtc tttggcataa aaccatgaag
    10561 catgaagacg atggaaagtt gccaatacat tttgggctcg cacatcattg ggattggcga
    10621 gggtcatcgc tgaggaacca ctgagtttcg ccgaatctaa aacctgattg caagcatcaa
    10681 tggcggtgag tttcccgctt ttgacttgct gaaaaagggg atccgtggtg gcaacccttt
    10741 gactggtcag ttgtgaatag cagcgatgaa aaagcgcgac ttcagataaa tcttcagctt
    10801 ggacaatttg gccaaaaaga aaaggagtga aaataaaaga aaaaatgacg gctttagata
    10861 aagccgttct ttctacaaaa cttcccattg cttaacatta ttttggttag tgaatatccg
    10921 caaatgttct caaaatggga gactcacttt aggacgatcc atgcgctgat taataagcag
    10981 cgttagctga gagacctacg tagaagtcat cggatggatg ctcgcctcct gactgttgca
    11041 ccaatttttg atattcaccc actagagcac agtcaatttt aagacgttga tccacatatt
    11101 tacagatatc gactttactc cccaatgcca ccagcttagt ttcactcgca acagatccca
    11161 agacattgac gttagccaac tgtttgatgc cacggaagtc tgtaaaaacg atgacccctg
    11221 gaacccaaga gttggttttt ttgtctaaaa tagtttgcgc gatcccgatg ctgtaagttt
    11281 cattgaagta agctttgcct gaatgggcat aggcaaagtt aagcatagtc ttggattttt
    11341 cattggtgat agtgccgcca aagctgtaac cggttgcctg atctccttga gttggatcaa
    11401 atcgattggt aatcgttgcc caaaactgac ccgtcaaact tttatttaac tttgcatcgg
    11461 ctctcatgga aagggccaag gcgttcaagg ctgtaacatc ctgatactta ttaaagacgg
    11521 cttttgaaac agctaatgat tgctgaatgt attgttgagc tgacatccat ggatcacgat
    11581 catgataacc tatgatattg accattagat tgatgtcttt agtgagtgag taagcgatgc
    11641 caattgaaga atagcctgtt gccgacaccg tattcccgcg ctcagcttcg ctggcatgtc
    11701 ctaaaatatt ttcccattga gataatccat tcgctttcaa ctcagcaccg agcatgggat
    11761 gagctttacc aaagctgaaa aggatcaatc cgggaggtgt atcactcata tttcccaaag
    11821 gattgattgg aataccgata gtggcatcca aagtttgaat gccggtttcc aattcaagtt
    11881 gagacatccg gctattgaca tggtcctctt ccatggcggc tttaatacca ggaacagctt
    11941 gggccacttg ggcgtcaatt tgtttatgaa ccaggggcaa gctggtttgt agcgccgcag
    12001 gtaagttctg tttgacttgg ccatcaatca gttgagcttt ttgacctgct gtcaacatat
    12061 taaggttagg attgctattc actgcggcgg tgattttcgc tctcaatcca tcttccacca
    12121 ttttaggagc catttgatag agggcctctc ttttctgtgc ggcaccttct tcaacttcag
    12181 catccaccgc cttgatggcg tcctttctag tttttcctcc cacgataaga ggactgacag
    12241 tacccgctaa gcggacttgc attataaatc cttcttggag ggagtagcct tgcttagtcc
    12301 ctagattttt tcttaagaca tccatcaatt ccgcgtcttg attcgaaatt tttaccgcta
    12361 aattttgctg agtcacatcc aattctgggt tgcttccttt gatcccagcc actgaactca
    12421 agctgacttg aatattaaga tcttgaacga ccttttttaa gaaagagaca ttcgagctcg
    12481 aaacattgga ttcggcgaag gattggttca aaaaattaaa tggattgatt gtagtccaca
    12541 agatagtggc tagtcccacg acgacgcgtt ttttattttt tttaaaaaat ccggatttgg
    12601 ctttgttcat ttggtcctcc tattgaagtt ataattttcg ctctcgtaat ggttgttgcg
    12661 attgttatgc caacgattta acgtgcttgg aaagtaatgg aagccataaa ggtgcttacg
    12721 agacgttcat ccttgcctta aattgttcct gtctcttttt gagacgaaat aaagaacttt
    12781 gagacaaaaa agctgcttgg attttcgcga tttcttccac tgttccgcga ctcttagtca
    12841 taaacctcct ggataatttt gtttatggta aatgatagat ttcaaataaa aagaatcatc
    12901 tgggccatcg atgcttttga agaaaaa
//
LOCUS       DATBTE010000334         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1341842, whole genome shotgun sequence.
ACCESSION   DATBTE010000334 DATBTE010000000
VERSION     DATBTE010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341842"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VIG33_12560"
     CDS             complement(<1..313)
                     /locus_tag="VIG33_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271714.1"
                     /translation="MKTPRSTLLTPPRKAFLRQLILGLSSVFLIMEPALAATSGFTYS
                     GKILDSNNAPVTATAVIFTVTVFDPAGKCWLYTEQRKLDLSQTAGTFSFEIGSSDIST
                     LY"
     gene            complement(610..>1520)
                     /locus_tag="VIG33_12565"
     CDS             complement(610..>1520)
                     /locus_tag="VIG33_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271715.1"
                     /translation="FTEPESSLRAVCISDDRAKSYLKKWDLKIENAGTFYKAQKPNSE
                     VYFKIEKSQSSLPSAADMATCYSLANSKLEIKDKDSELAFRNLCDSHATKVTLGDLKA
                     GVKDQCIDAGSDGITDKNILAMNLPAVESINIKSNKYLKYPEKARIALFEKSHKQEVA
                     YRNLGCDKIKVEISKCNKEKCSPSDFAHFAGEFDKSLGDIDVREEKNKKTLIELDQQL
                     SKLQQILKVSPEKELSDVNTKIEKITEQKRLLAYDLDKATAPVLNATLNEILNLNAFG
                     KCCANSECKRSMFDKYAPSEPKPGRQ"
BASE COUNT          408 a          302 c          291 g          519 t
ORIGIN      
        1 cataaagagt tgagatgtcg ctggagccaa tttcaaagga aaaggttccg gcggtttggg
       61 ataaatccaa ttttctttgc tcggtataaa gccagcattt cccggcggga tcaaagaccg
      121 tgactgtaaa gatcaccgca gtggccgtga ccggagcatt gttggagtct aagattttac
      181 cgctataagt aaagcctgaa gtcgcggcca gcgcgggctc cataattaag aatacggaag
      241 acagccctaa aataagctgc cttaaaaagg cctttcttgg aggtgttagt aacgtactac
      301 ggggagtttt catattctaa aatatcggaa aaaataccca attccttgag gtttttcctt
      361 gcaaaaaagg ttggcaattc tctttttgaa gctgggggct ttgctatatg aataaattaa
      421 tatttattta ctagataatg cagcagccaa tggggcaaaa gtccgatgta gataccttgt
      481 ccagctatga agaagtgcct tggtttggga atagcttccg ccgttgaact cagcttcgtc
      541 cttgattcga atattcgaaa tcttaactta aacctttcgg ctcctataat attatcttct
      601 ttagacttcc tattgccttc caggcttggg ttctgatgga gcgtacttat caaacatgct
      661 tctcttacat tcgctatttg cacagcattt cccaaaagca tttaaattta aaatttcatt
      721 aagggttgca ttgagaacgg gggcagtcgc cttatcaagg tcataggcta gcaatctttt
      781 ttgttctgta attttttcga ttttagtgtt aacatcagac aattcttttt caggggaaac
      841 ttttaagatc tgctgtaatt tagaaagttg ctgatctaat tctattaagg ttttcttatt
      901 tttttcttct cttacatcta tatcacctaa acttttgtca aattctccag caaaatgtgc
      961 aaaatctgaa ggtgagcatt tttctttatt acacttggaa atctctactt ttattttatc
     1021 acagccaaga tttctataag caacttcttg cttgtgagac ttttcaaata aagcaattct
     1081 agctttctca gggtatttta aatatttatt gctttttata tttattgatt ccactgcggg
     1141 aagattcatg gctagaatat tcttatcagt tattccatct gatccggcat caatgcattg
     1201 gtcttttacg cccgctttga ggtcccccaa agtaactttc gtggcgtgag aatcacacag
     1261 attcctgaaa gcaagttcac tgtctttatc tttaatctct aatttggaat tcgctaagct
     1321 atagcaagtt gccatatccg ccgcgctggg caagctgctt tgagatttct caattttgaa
     1381 ataaacttcg ctatttggct tttgcgcttt ataaaaagta ccagcatttt ctatctttaa
     1441 atcccatttt ttgaggtaac ttttcgccct atcatcgcta atgcaaacgg cgcgcagact
     1501 gctttccggc tcggtgaagg
//
LOCUS       DATBTE010000335         1643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_249906, whole genome shotgun sequence.
ACCESSION   DATBTE010000335 DATBTE010000000
VERSION     DATBTE010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249906"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1140
                     /locus_tag="VIG33_12570"
     CDS             256..1140
                     /locus_tag="VIG33_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271716.1"
                     /translation="MNGNSVSEGPAIPLGKYQVKFYSCSISGQDWIGVREFVSRGKTY
                     VLLIQPNNLQTQIAELSCLKCTPISLEDLPESPYKRGLKEELSPPFPQVNDGLTHAKS
                     DAGLFLTVDLCPSRQNFDASIFENAKVKSREAFPVAVALSGGWMNHFDTEFQWMKNQA
                     LDHRLDIIWVNHSYTHPYKKGIKNKNNFLLTSGVHFDEEVLKQEQYMISRGILPSVFF
                     RFPGLVSNQDLMIRLAHFGLIALGTDAWLALGQKTKPGSIVLIHGNGNEEGGVHLFIK
                     LLDTISDMGVFKNLNLVL"
     gene            1235..>1643
                     /locus_tag="VIG33_12575"
     CDS             1235..>1643
                     /locus_tag="VIG33_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigmaB regulation protein RsbU"
                     /protein_id="HEY8271717.1"
                     /translation="MAKEIDSLKERIQELELELVGKDKELLKYRQELGKVNVSLEHLI
                     AQISEEIRMAGLIQRMLSPTQLPNISGFECSTKYLPGERFGGDYFDIFEHEDKLKFGI
                     VVASSSGYTMSALFLSVLIKISSQIEARRGLEPH"
BASE COUNT          448 a          317 c          377 g          501 t
ORIGIN      
        1 ttcaggtcct gatctgatga atgttagtga gggagccgta tgccttagtt gggcaagtac
       61 ggttctaagg gggggggcgg gagtatgtaa agaggcacag agatgttcaa aaaatcttaa
      121 atccttcact gatatgttat attttcctga attatcataa ataccatgcg taagcttatc
      181 attataacaa ttattttgtt aacgagcttt caggtgaagg cagaagagag tattccctct
      241 tcctgcatgc tttttatgaa tggaaattct gtctcagagg gtcccgcaat tcctttagga
      301 aagtaccagg tgaaatttta ttcctgctcc atctccggtc aagattggat tggggtgcgt
      361 gagtttgttt ctcgcggaaa aacatacgtc ttattaattc aaccaaataa tttgcagact
      421 caaatagccg agctcagttg tctaaaatgc actcccatca gccttgaaga ccttcccgaa
      481 agcccttata agcgagggct caaagaagag ctttctcctc cctttcctca agttaatgac
      541 ggtctcactc atgcaaagtc tgatgctggt ttatttctga cagtggattt atgtccttcc
      601 cgtcagaatt ttgatgcttc aattttcgag aatgcgaaag tgaaatcccg agaagcattt
      661 cctgttgcgg tggcgttaag tggcgggtgg atgaaccatt tcgacaccga atttcagtgg
      721 atgaaaaacc aggctttgga tcatcgcttg gatatcattt gggtaaacca ctcctataca
      781 catccttata aaaagggaat taagaataaa aataatttcc ttttaacttc aggagttcat
      841 tttgatgaag aagtccttaa acaagagcag tacatgattt caaggggaat tctgccgtcg
      901 gtattttttc ggtttccagg actggtttcg aaccaggatt tgatgattcg tctggctcat
      961 tttggcctca tcgccttggg aaccgatgct tggttggctc ttggacaaaa aaccaaacca
     1021 gggagtatcg tacttattca tggaaatgga aatgaggaag gaggagtgca tttatttata
     1081 aagcttctag ataccatttc cgatatgggc gtctttaaaa atcttaattt ggttttgtaa
     1141 ttaaacaggg cctaggtcgt gaaaagctct tagtcgatgc ctcccagagt tccttgcttg
     1201 gccctgttag ttttcggttt taatatatga tccgatggct aaagaaatag actctctcaa
     1261 agaacgtatt caagaattgg aactggaact tgtcggcaaa gacaaagagc ttttgaagta
     1321 ccgtcaggaa ctcggaaaag tgaatgtttc ccttgagcat ttaatcgcgc aaatcagtga
     1381 ggaaatccgc atggctgggt tgattcagcg gatgctctct cccactcaat tgccaaatat
     1441 cagcggcttt gaatgcagta ctaagtattt gccgggggaa cggttcggcg gggattattt
     1501 tgatattttt gaacatgaag ataagctcaa attcggcatc gtggtggcga gttcttctgg
     1561 ttacactatg tccgcactat ttctttcagt gcttattaag atttcttcgc aaattgaggc
     1621 gcgacgaggc ctggaacctc ata
//
LOCUS       DATBTE010000336         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1412045, whole genome shotgun sequence.
ACCESSION   DATBTE010000336 DATBTE010000000
VERSION     DATBTE010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412045"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VIG33_12580"
     CDS             <1..935
                     /locus_tag="VIG33_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8271718.1"
                     /translation="YINWDGVMKKSARVVVQASLSPGRSGEQIRGMDGLFSYLYDEIH
                     RMDSPRYPKTAQLDPEKVARGEKAFNLNCADCHGTHSENGIYYRDPKDFKDPKKGHYV
                     DEIISTKHLQTDETRLKGMNLPYINTVEKTWLGHYGDSNYAFRTRQVLFDYLNKTNLS
                     DAPIREKISQLCEKQTDEDCTYSLTEEMKKKVYGYQVPPLWGIWASAPYLHNGSIPNL
                     RGVLFPQERPKTWKLVDDPLGINTPFTPYNIDNPGLKVEVNLTASPGNTKIYNTESPG
                     CSNSGHDIQDWTQKPAASIKDATKEDILEYLKTL"
     gene            999..>1715
                     /locus_tag="VIG33_12585"
     CDS             999..>1715
                     /locus_tag="VIG33_12585"
                     /inference="COORDINATES: protein motif:HMM:NF024646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271719.1"
                     /translation="MKIKWLLAISILSGVMSIEIDIANANQETISTLTSIKMEELSSI
                     IGEAKTNLMSKKIKDPSGEYWSMPGYLGTHYVSQYYLFTRWFGIQLPAPYKENLLNLI
                     LSRQNKKGGFWLALEDANLSSPNAIDPSIYHYWALKVLKTEFPQRQVEIDTALTKAKI
                     YILKSGGAENSVLFTKVVMALFGNYSWDNIPYIPSVTYNALISPFVVDKFSQWVIPHL
                     KPLGYIRAHRIAKSDLGAEFS"
BASE COUNT          552 a          355 c          351 g          457 t
ORIGIN      
        1 aatatattaa ttgggatgga gtcatgaaaa agtccgcacg tgtcgtggtg caagctagct
       61 tgtctccagg ccgaagtgga gaacaaatcc gcggaatgga cggtcttttt tcttatcttt
      121 atgatgaaat tcacaggatg gattcaccaa gatatcccaa aacagcccaa cttgatcctg
      181 aaaaagtggc gcgcggggaa aaggctttta acttgaactg cgctgattgc catggaaccc
      241 attctgaaaa tggaatctat taccgagatc caaaggattt taaagatcca aaaaagggtc
      301 actacgtaga tgaaatcatc tcaacaaagc accttcaaac agatgaaact cgcttgaaag
      361 gcatgaattt gccttatatc aatactgtgg aaaaaacttg gttgggccac tatggcgatt
      421 ctaattacgc gtttcgaaca cgccaagtat tattcgacta tctgaataaa acaaatctat
      481 cggatgcgcc aatcagggaa aaaataagtc aactctgcga aaagcaaacg gatgaggatt
      541 gtacttacag ccttactgaa gaaatgaaaa agaaggtcta tggttatcaa gttcctccgc
      601 tttggggaat ctgggcttca gcaccttatc tgcataacgg ttcaatcccc aacttaagag
      661 gagtgctctt tccgcaagag cgtcctaaaa cttggaaatt ggtggatgat cccttgggta
      721 tcaatactcc attcactccc tataatatag acaatccggg attaaaggta gaagtgaatc
      781 tgacggcatc acctggaaat accaaaatat acaacactga aagccccggc tgttcgaatt
      841 cgggtcatga cattcaggat tggacgcaaa aacccgcagc gtcgatcaag gatgcaacta
      901 aagaagatat tttggaatat ttaaaaaccc tttaatcaga gaaatatttc aagcattaca
      961 gaaaaacaaa tcgctgggtc gccagcggga aatgatttat gaaaataaaa tggttgctcg
     1021 caatatcaat tttatcagga gtcatgtcga ttgaaattga tattgcgaac gccaatcaag
     1081 aaacaatctc cactcttact tcaataaaaa tggaagaact ctcttctatt atcggggagg
     1141 ctaaaacaaa cctgatgtcg aaaaaaataa aggatccgtc tggtgaatat tggtccatgc
     1201 ccggctatct tggaactcac tatgtctccc aatactattt atttacccga tggtttggaa
     1261 ttcaattgcc cgccccctat aaagaaaatc tgttgaatct tattttatcg agacagaaca
     1321 agaagggggg gttttggctg gccctggagg atgcaaacct cagctctccc aatgcaattg
     1381 atcctagtat ttatcattat tgggcactca aggttctaaa aacagagttt ccccaacgac
     1441 aagttgaaat tgatactgca ttaacaaaag caaagattta tattctcaaa agcggtggtg
     1501 cggagaattc cgtcttgttc acaaaagtgg ttatggctct tttcggcaac tattcctggg
     1561 ataacatccc ctatatcccc tcagtgacct ataatgcttt aatttcccca tttgtggttg
     1621 ataaattttc gcaatgggtg attccgcact tgaagccctt aggctacatt cgagcacacc
     1681 gaatcgccaa aagtgattta ggagctgaat tttca
//
LOCUS       DATBTE010000337         2805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1480551, whole genome shotgun sequence.
ACCESSION   DATBTE010000337 DATBTE010000000
VERSION     DATBTE010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480551"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..2203
                     /locus_tag="VIG33_12590"
     CDS             77..2203
                     /locus_tag="VIG33_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271720.1"
                     /translation="MNKDVKRELVEVYQRYGFEPAKTYENDKVLVFTIKTGYFDNADI
                     VPLVEGINVQQAFDDYSKAGFACTVRHVQTPNQTERQLFKGFFSVESILTRLDYDYER
                     FTKGIVAPFSADAQYEYINAPYRVNGQPGNLSPAEEVATRLDSHKPILFLIEAAAGFG
                     KTCTAYELVHLLTQRGEYLPLFSELARNRQAVIFRYILLDEIDRTFPVLSSRLVQTEM
                     KNGRVITILDGFDELLRKTEEGNDFENKEPMLETIGEFLTGNAKIVLTTRRTVLFEGD
                     AFHTWVERHSDDFDLVRIRISEPRVDNWLDKSRLSKLKETGLRIKDIANPVLLSYLRC
                     ISDVDFEKASSEPHLLVEKYFDFMLDREKSRQDLRLNPDKQQEVLCSIAEDMIAFGYT
                     SEHRDYIVDHIQRVNSNIIDEALSAYPAAEKPTKEGVANKLASHALLDRSQREPNKIG
                     FINEFVLGHFVAKIILRTSDWLNDDLRFIEPAVISFQPRSEESRILLWEKLKNSVEFL
                     PISYQLDITLRLTGSIPFDLCNDEANGLEISQAVIGSKLISNFQFNECIFRNCVFDLS
                     NITNATFLNCKYYGNSFLNSNAKGQIHILGAFGDDNFIDTLISSNTPISEEIEPDRSI
                     LLERFILEKFWPVGREAVMHRHRPIKGVCVNNGEFKTNELCSAIASLKKKGILKEPMQ
                     SAFIEINLDEIQAIREILGRNLVNGK"
     gene            2193..>2805
                     /locus_tag="VIG33_12595"
     CDS             2193..>2805
                     /locus_tag="VIG33_12595"
                     /inference="COORDINATES: protein motif:HMM:NF016488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HEY8271721.1"
                     /translation="MESNFKHTAENVRRDIEAILDKVCLLCRVFARGKDDRSLTKKLH
                     KDEGKYSLHGKLIQDAIGVRVALYFEEDVDIVAQLLSSEFEIDNNASTVDTHATDQFT
                     VTRHNLIFRLPEYYISDMQRSIGSMPIDTTFEVQLRSILSEGWHEVDHDLRYKSKSSW
                     NGNDDLSRALNGILATIETAEWGMRRIFDDLAYRHYKKKNWETM"
BASE COUNT          898 a          516 c          588 g          803 t
ORIGIN      
        1 ctaaactgca atatcgcact cgtatttgaa gattataaaa aagttgtcga gaaagctata
       61 taaattagga tggggaatga acaaagacgt taaaagagag ctcgtagagg tttaccaaag
      121 gtatggcttt gaaccggcaa agacgtatga gaatgataag gttctcgtct tcactataaa
      181 aacaggctat tttgataacg ctgatatagt ccctttggtt gaaggtatca atgttcaaca
      241 agcatttgat gactactcaa aagcaggatt tgcttgtact gttagacatg ttcaaactcc
      301 caatcaaacc gaacgccaac tttttaaagg ttttttttcg gtagagtcga ttcttaccag
      361 acttgactat gattatgaac gattcactaa aggtattgtc gccccgttct cagctgatgc
      421 tcagtacgaa tatataaacg caccctatcg ggtcaatgga caacctggaa atctttcacc
      481 agcagaagag gttgcaacga ggcttgattc acataagcca attctctttc taatagaagc
      541 ggcggctggc tttgggaaga cgtgtacagc ttatgaatta gttcaccttc ttacacaacg
      601 tggcgaatat cttccccttt tttcagagtt ggcaagaaat cgacaagcgg taatttttag
      661 atacatttta ttggatgaaa tagatagaac attcccggtg ctaagttcaa ggctagtcca
      721 gactgagatg aagaatggaa gagtaataac tatacttgat ggtttcgatg agttacttcg
      781 aaagactgaa gaagggaacg acttcgaaaa taaggaacca atgctggaaa ccatagggga
      841 gtttctaacc ggtaacgcga aaattgtatt aactactagg cgaaccgtgc ttttcgaagg
      901 ggatgcattt cacacttggg ttgaaaggca ctcagatgat tttgatctgg tgagaattcg
      961 aataagtgaa ccgcgtgtcg ataattggtt agacaaatca cgtcttagta agttgaaaga
     1021 aactggttta cgaatcaaag atatcgccaa tccagttctc ctctcctacc ttcgttgtat
     1081 ttctgatgtg gattttgaga aagcatcttc agaaccacac ctcttagttg agaagtattt
     1141 cgactttatg ctcgatagag aaaagagtcg tcaagaccta cggttaaacc ctgacaagca
     1201 acaggaagtc ctttgctcca ttgccgaaga tatgattgct tttggttaca cctcggaaca
     1261 tcgcgactac atagtcgacc atattcaacg ggtgaattca aatattattg atgaagcatt
     1321 gtcagcttat cctgcggcag aaaaaccaac taaagaggga gttgcgaata aattagcaag
     1381 tcacgcacta ttagacagaa gtcaaagaga gcctaacaaa atcggattta ttaatgaatt
     1441 tgtattaggg catttcgtcg cgaagataat actaagaact tctgactggt tgaatgatga
     1501 ccttcgcttt atagaaccag ccgttatttc ttttcaaccg cgatctgaag aatcacgaat
     1561 tttactttgg gaaaaactaa aaaactctgt cgagtttcta ccaatatcct accaacttga
     1621 cattacttta cgtctaacgg gctctattcc tttcgattta tgtaatgatg aggcaaatgg
     1681 cctagaaatt agtcaagctg ttattggaag taaacttatt tccaacttcc agtttaatga
     1741 atgcatattt aggaattgcg ttttcgactt atcgaatatt actaacgcta catttcttaa
     1801 ctgtaaatac tatgggaaca gttttttaaa ttcaaacgca aaaggtcaaa ttcatatcct
     1861 tggagcgttt ggtgatgaca attttattga cacactgatt tcctctaata cccccatatc
     1921 agaggagatt gagccagacc gcagtatact attagaacga tttatccttg aaaaattctg
     1981 gccagtggga cgtgaagctg tcatgcatag gcatcgcccc atcaaagggg tctgtgttaa
     2041 taacggtgaa ttcaaaacca atgaattgtg ttcagcaatt gcatccctca aaaagaaagg
     2101 gattcttaaa gagccgatgc aaagcgcatt tattgaaatt aatctggatg aaatccaggc
     2161 tattcgagaa attcttggtc ggaacttagt taatggaaag taattttaag cacactgccg
     2221 aaaatgtacg gcgagatatt gaagcaatcc ttgataaagt ttgcctttta tgtagagttt
     2281 ttgctcgtgg aaaagatgat cgatcactaa ccaaaaagtt gcataaggat gaaggaaagt
     2341 attcccttca tgggaaactg atacaagacg caattggggt gcgagttgca ctctattttg
     2401 aggaagatgt tgatattgtt gcccaacttt tatcctcaga atttgaaatc gacaataacg
     2461 cctctacggt cgatactcac gcaacagatc aatttacggt tacccgtcac aatcttatat
     2521 ttcgactacc tgagtactat attagcgata tgcagcgaag tattggcagc atgccaatag
     2581 acactacatt cgaggtgcaa cttagaagta ttctttctga agggtggcat gaagtagacc
     2641 acgatcttcg atacaaatca aaatcaagct ggaatggtaa tgatgatttg agtagggcat
     2701 taaacggaat tttggcgaca atagaaactg ccgaatgggg tatgcgtaga atcttcgacg
     2761 atcttgcgta tcgccattat aagaagaaaa actgggaaac aatgt
//
LOCUS       DATBTE010000338         6970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_181656, whole genome shotgun sequence.
ACCESSION   DATBTE010000338 DATBTE010000000
VERSION     DATBTE010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181656"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2081
                     /gene="lptD"
                     /locus_tag="VIG33_12600"
     CDS             <1..2081
                     /gene="lptD"
                     /locus_tag="VIG33_12600"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEY8271722.1"
                     /translation="GIIYNGFVQSGPVVFSGSILQKASEEEYYVSDADYTTCTNCPPT
                     WGFSGSNIRAVLGGYAYIKNSFLKIYSVPVFWLPYLIVPLKSDRQTGFLTPRLLGDQA
                     GGLALSESFFWAISRSTDATFALTNYEKRGLKGSAEYRYLLNENSHGTFNAAALVDHV
                     FRDDLRVTNFEKEGEKGKDITRWYAKYDHYYDLPNGYIQRAQINIASDLQYPKDFSEE
                     TLNHGDPAMENRISLTKNSFDQHFSVDTSYYVNLLQSDPLASNSNSVHRIPEIRFSQT
                     QQKISNSQFLFSYDLDYVNFARSGPSFDKLDTDNGVSFEKNSCRNPKYDQYPNCHLSL
                     QGKFDPATDLLRTGQRIDMSGTIARPLNIGDYLNILPALSYRETDYQFNVGDERTNSR
                     RYLRATLGAKTSISRVFILDEAPKADKYKHEIIPEVIATTIPWIKHPAHSFFGTGIDP
                     DGTFYYSDYLTDADLASPTRLQFDYNDRIYDRGQVTFAVNNKLVQKAWVGDTPTYLQI
                     ASLKFFQSYDTYQANLRNPTREPWSDVTTILDVRLKHFQTYTILNYFPQQNVTNISSR
                     VRINNDNDQFVQVSLTKTYKISPGVDVDPNSRVEDYAFAAGTTGSYINFMGKFVYDAN
                     WANTPSHKRIKEWAYMAQIKPPGDCWAINLIQYFPTDGNTKTFVDFVFNFDGTPKPPL
                     PTENLNLYGF"
     gene            complement(2078..2509)
                     /locus_tag="VIG33_12605"
     CDS             complement(2078..2509)
                     /locus_tag="VIG33_12605"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEY8271723.1"
                     /translation="MIKAIPRPFIKKTSRTTVLASGTEEPSQALKNTSLCKSHVQGHW
                     AEKQVVDYFLKRSFRVLKIRWRTTYAEIDLVVESPIPEIWIVEIKSLSHFEFLEVRVS
                     KKQKERLKRAHLFVQSKTIKSVRLVLAFVDKKGEVLIIEEF"
     gene            complement(2400..3047)
                     /locus_tag="VIG33_12610"
     CDS             complement(2400..3047)
                     /locus_tag="VIG33_12610"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEY8271724.1"
                     /translation="MKRLANDKAKVFELIDWRSFSVADPHYAIIGVDEVGRGCLAGPV
                     YAAAVSFNSDILSDLVTDSKLLSEKRRNELAIEIKKHHQVGLGFATVEEIDDINILQA
                     SLLAMKRAVQNLGRDSGHLLIDGNKTISGMSAKFKQTTIVKGDLRVSLISAASIVAKV
                     TRDQFMKDLGLQFPVYGFEDHKGYSTAVHKKNIADHGPCIWHRRTFSGVKEHLSL"
     gene            complement(3056..3472)
                     /gene="rplS"
                     /locus_tag="VIG33_12615"
     CDS             complement(3056..3472)
                     /gene="rplS"
                     /locus_tag="VIG33_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEY8271725.1"
                     /translation="MAKAAKKTTAKTAGKETNLVRRVTQRASNKNINTFKSGDTVNVY
                     VKVKEGEKERIQLYKGIVTKIQGNGVAKSFTVRKISAGVGVERTFPFVSPAIDRVETV
                     AHGKVRRSKLYFLRDLEGKAAKITSEIATKESATAE"
     gene            complement(3508..4089)
                     /locus_tag="VIG33_12620"
     CDS             complement(3508..4089)
                     /locus_tag="VIG33_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY8271726.1"
                     /translation="MSDTPYVPRLAVGLLHYPILDKEKKIISTNITNFDIHDIARASR
                     MYSVENYYLIHPMQEQLMFVERVLDHWRTGLGAKFNPSRRTALARVKTAESFEMAFKD
                     WGVPEAMTVATHARPIEGVATLSFRSIREKMHDEKKPCFLLFGTGYGMTEDFMKSCTA
                     ILEPIKGAPPDDFRHLSVRSAVSICLDRIMGSW"
     gene            complement(4082..4876)
                     /gene="trmD"
                     /locus_tag="VIG33_12625"
     CDS             complement(4082..4876)
                     /gene="trmD"
                     /locus_tag="VIG33_12625"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEY8271727.1"
                     /translation="MAFQFQVVTVFPEMIQAAMSAGVFFQAQKNSLLTLSCINPRDFT
                     SDVHRTVDDRPFGGGDGMIMLPEPLAKSIAMAKSSQPFSKVIYLSPQGKPLTHKKAQA
                     LSQEQGLILLCGRYGGIDQRVINQFVDEEISTGDYVISGGELAACVLMDAVSRFLPGV
                     LGHRESAEKDSFAEGLLEHPNFTRPREYQGQNVPEVLVNGNHKKIEDWKHLVSYLVTL
                     QKRPDLIEKNKLSRSLKEAHKLWQSLTDAEREVLGLTRLKEKDFYE"
     gene            complement(4876..5397)
                     /gene="rimM"
                     /locus_tag="VIG33_12630"
     CDS             complement(4876..5397)
                     /gene="rimM"
                     /locus_tag="VIG33_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164563.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEY8271728.1"
                     /translation="MEMKLVGKVKDAHGLRGELYILVFSGDVSWAKKLKTFELRSPRT
                     GEIASYAVEKMKPFKQGLIVKSPLMMDRNAAEAVKGFEFSIPADIFVSKPGETIFLNE
                     IMDFMVKTAAQENVGLIKGFSSNGVQDLLVVDAAKGRVEIPFVEAFIKKIDFKHHTIV
                     MDLPEGLLDLENL"
     gene            complement(5537..5776)
                     /locus_tag="VIG33_12635"
     CDS             complement(5537..5776)
                     /locus_tag="VIG33_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEY8271729.1"
                     /translation="MENLKDLVEFMAKSLVDKPEHVEVDEIPGQQTTLLALKVDKEDL
                     GKVIGKQGKTAAAMRTIIRAAGTKLNKRYHLDIVE"
     gene            complement(5845..6096)
                     /gene="rpsP"
                     /locus_tag="VIG33_12640"
     CDS             complement(5845..6096)
                     /gene="rpsP"
                     /locus_tag="VIG33_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEY8271730.1"
                     /translation="MAVVIRLARTGTKHTPKYRITVADSRRYVTGKHLEVIGTYIPTP
                     KGADQKVKIDLEKYNEWIKKGAQPSERVKHVVKLVQAAK"
     gene            complement(6205..>6970)
                     /locus_tag="VIG33_12645"
     CDS             complement(6205..>6970)
                     /locus_tag="VIG33_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY8271731.1"
                     /translation="RLQIDDQLMSELQNLKQVWSPQEVLLVADAMLGQQSVNVAEGFH
                     QRLGLTGLVLTKVDGDARGGAALSIREVTGIPIKFLGVGEKVAALEIFHPDRLASRLL
                     DMGDVLTLVERAQEVIDEKSARESARKMAKNEFDLEDFLAQIQQLKKLGGMESLLKFL
                     PGMGDLSKQLKNMTPPDQEIKKLESIIRSMTPQERKNHKILNASRRQRIANGSGTKVQ
                     DVNKLIKQFEDAKKMMSGLMKMGMGRGGGGGMKFPF"
BASE COUNT         1982 a         1682 c         1301 g         2005 t
ORIGIN      
        1 caggaattat ttataacggt tttgttcaat caggtcctgt tgttttttca ggcagtattt
       61 tacaaaaagc cagcgaagaa gaatattatg tttcagatgc agattacacg acctgcacca
      121 attgccctcc aacctgggga ttttcgggga gcaatattcg cgctgtattg gggggctatg
      181 cttacataaa aaattctttt ctgaaaattt attctgttcc cgttttctgg cttccttatt
      241 tgatcgtgcc ccttaagagt gaccgccaaa caggattcct tacgccccgt cttttaggcg
      301 accaagcggg tgggctcgca ctttctgaga gctttttttg ggctatttca cgcagtacag
      361 atgccacttt cgcccttacc aattatgaaa aacgcggact taaaggctct gctgaatatc
      421 ggtatttgct gaatgaaaat tcgcatggaa cttttaatgc cgccgccctg gtggatcatg
      481 tcttcagaga tgacctccgg gtcactaatt ttgaaaaaga gggcgaaaaa ggcaaagaca
      541 taactcgatg gtatgctaaa tacgatcact actatgattt gccaaatggc tatattcaac
      601 gagcgcaaat caatattgcc agtgatttac agtaccctaa agacttctcg gaagaaaccc
      661 tcaatcatgg cgatcctgca atggaaaacc gaatctccct caccaaaaat agtttcgatc
      721 aacatttcag cgtggacact tcctactatg taaatctgtt acaatctgac ccactggctt
      781 ccaactccaa ctcagttcat cgcatccctg aaattcgctt ttcccaaact caacagaaga
      841 tcagcaactc tcagtttctt ttttcctacg atctcgacta tgtgaatttt gcaagatcag
      901 gcccctcctt tgataagcta gacaccgaca atggggttag ttttgagaaa aacagctgta
      961 gaaatcctaa atatgatcaa tacccaaact gtcacctatc gttacaagga aagtttgatc
     1021 ccgccacgga tctgctgcgc accggccaac ggatcgatat gtctggcacc atcgcccgtc
     1081 ctcttaatat cggcgattat ttaaatatcc ttccggctct ttcctatcgt gaaacagact
     1141 atcaatttaa tgttggcgac gaacgcacca acagtcgccg ttatcttcgc gcaaccctgg
     1201 gagctaagac ttccatcagt cgcgttttta tcttagatga agctccaaag gcggataaat
     1261 ataaacatga aattatcccc gaggtgattg ccacaactat tccttggatc aaacaccccg
     1321 cacactcctt ctttggcacg ggcatagatc ctgatggaac tttttattat tcagactact
     1381 taacagatgc cgatcttgcc tctccaactc gccttcaatt cgactataat gatcggattt
     1441 atgatcgtgg ccaagtcact tttgccgtaa ataacaagct tgtgcagaag gcatgggtgg
     1501 gcgacactcc cacctattta cagattgcca gtcttaaatt ttttcaatcc tatgacacct
     1561 atcaagccaa ccttcgcaat cccacccgtg agccttggtc tgatgtcaca acgatcttag
     1621 atgtgcgcct gaagcatttt cagacctata caatactcaa ttattttcct cagcaaaacg
     1681 tgactaatat ttcttcacgg gtacgaataa ataacgacaa tgatcaattc gttcaagtgt
     1741 ctctcactaa gacttataag atcagccctg gagttgatgt tgaccctaac agccgcgttg
     1801 aggactatgc ctttgctgcc ggcactaccg gaagttatat caactttatg ggaaagtttg
     1861 tttacgatgc gaattgggca aacacccctt cccataaaag aattaaggaa tgggcctata
     1921 tggcgcaaat caagccccca ggggactgct gggcgatcaa cttaatccag tatttcccga
     1981 cagacggcaa tactaaaact tttgtcgact ttgtctttaa ctttgatggc acgcccaaac
     2041 cacccctccc cactgagaac ctaaatctct atggatttta aaactcctca atgatcagga
     2101 cctcaccttt tttatccaca aaagcaagaa ccagccgcac agattttata gttttacttt
     2161 gcacaaataa atgcgcacgt ttgagccgct ccttttgttt tttagaaacg cgcacttcaa
     2221 gaaactcaaa gtggctgaga cttttaattt caacaatcca gatctcaggg ataggactct
     2281 ccacaaccaa atcaatttcc gcataggtcg tacgccaccg gatctttaaa actctaaagg
     2341 atcttttaag aaaataatct acaacctgtt tctcagccca gtgaccttga acatgggact
     2401 tacaaagaga ggtgttcttt aacgcctgag aaggttcttc ggtgccagat gcaaggaccg
     2461 tggtccgcga tgtttttttt atgaacggcc gtggaatagc ctttatgatc ttcgaatcca
     2521 tagactggga attgaagccc caaatccttc atgaattgat cgcgggtgac tttcgcgaca
     2581 atagaagctg cagaaatcaa ggagactcta agatcgccct ttacaatcgt ggtctgttta
     2641 aacttcgcag acattcctga aatggtctta tttccatcga tcagcaaatg ccctgaatct
     2701 cttcctagat tctgcacagc ccgcttcatt gcaagcaaag aagcctgaag aatattgata
     2761 tcatcaattt cctcaacagt agcaaaccct agaccaacct ggtggtgctt tttaatttca
     2821 atagcgagtt cattgcgacg cttttcggac aaaagcttcg aatcagtaac taagtcggat
     2881 aaaatatctg agttgaatga gactgcggcc gcatagacag gaccagcaag acaacctctt
     2941 cccacttcat cgaccccgat tattgcataa tggggatcag caacagagaa acttcgccaa
     3001 tcgataagct caaaaacttt agctttatca ttggcgaggc gtttcattct gcccactact
     3061 cagcagttgc agattcttta gtcgcaattt cagaagtaat cttagcggct ttaccttcaa
     3121 gatctctcaa gaaatacaac ttagaacggc gaactttacc gtgagcaaca gtttcaacac
     3181 ggtcaattgc agggcttacg aacgggaatg ttctttccac gccaacaccc gcagagattt
     3241 tacggacagt gaaagatttt gcaacgccat taccttggat cttagtaacg atccctttgt
     3301 aaagctgaat gcgttctttt tcaccctctt ttacttttac atacacgttc acagtatcgc
     3361 ctgatttaaa cgtattaata tttttattag aagcacgttg agttacacgg cgaacaaggt
     3421 ttgtttcttt accagcggtt tttgcagtcg ttttttttgc agctttagcc atttttttcc
     3481 ccatggatat aaaagtaaag gaaggtgtta ccatgacccc attatacggt caagacagat
     3541 acttacagca gatcggacag aaagatgacg aaaatcatcc ggcggagccc cttttatagg
     3601 ctctaaaatg gccgtacagg atttcataaa gtcctcagtc attccataac ctgttccaaa
     3661 tagaagaaaa cagggctttt tctcatcatg catcttttcc cggatacttc gaaagctcag
     3721 ggtcgcaacc ccttcaatgg gacgggcatg ggtcgccacg gtcatagcct caggaacccc
     3781 ccaatcttta aaagccattt caaagctctc tgctgtttta acacgcgcca aggcggtcct
     3841 gcgagagggg ttgaatttag ctccaagccc ggttctccaa tgatccaaga ccctttcaac
     3901 aaacatcagt tgctcttgca tcggatgaat caaatagtaa ttttcaaccg aatacatacg
     3961 agaagcgcgg gcaatatcgt gaatatcaaa atttgtaata ttcgtcgaaa tgatcttctt
     4021 ttctttatct aagataggat aatgaagaag acccactgca aggcgaggta catagggagt
     4081 atcactcata aaaatctttc tccttaagtc tggtaagacc tagaacctct cgttcagcgt
     4141 ccgtcaagga ttgccagagc ttgtgagctt ctttgagtga ccgagacagc ttattttttt
     4201 cgatcaagtc cggacgcttt tgcagagtca ccaagtagga tactaaatgc ttccagtcct
     4261 cgatcttttt gtgatttccg ttcaccaaaa cttcaggaac attctgtccc tgatactccc
     4321 gcgggcgcgt aaaatttgga tgttccaaaa ggccttcagc aaaactatcc ttttccgctg
     4381 attctcgatg tcctaggacc cctggaagaa atctagacac cgcatccatt aatacacagg
     4441 cggcaagttc ccctccggaa atcacatagt ctccggtgga aatttcctca tctacaaact
     4501 gattaatcac tcgttggtca atgcccccat aacgaccaca aagtaaaatt aacccctgtt
     4561 cctgagagag agcctgggcc tttttatggg ttaacggttt tccttgaggt gataagtaaa
     4621 taactttaga aaaaggctgt gatgatttag ccatagcaat ggatttcgcc aagggctcgg
     4681 gcaacatgat catcccatcg ccaccgccga agggcctatc atccaccgta cgatgaacat
     4741 ctgaagtaaa gtctcggggg ttaatacaag ataatgttaa taaggaattt ttttgtgctt
     4801 gaaagaacac tcccgcagac attgcggctt gaatcatttc cggaaaaaca gtgactacct
     4861 ggaattgaaa agccactaca aattctcaag atctaaaaga ccttctggta aatccatgac
     4921 gattgtatgg tgtttaaaat caattttttt aatgaacgct tctacaaagg gtatttcgac
     4981 acgtccctta gccgcatcta caacaagaag atcttgaacc ccattagaag aaaagccttt
     5041 aattagacca acattttcct gagccgcagt ttttaccatg aagtccatta tttcatttaa
     5101 gaagatggtt tctcctggtt tggaaacaaa aatatcagca gggattgaaa attcgaagcc
     5161 tttgacggct tcagcagcat tacgatccat cataagcgga gattttacga tgagaccttg
     5221 cttaaaaggt ttcatttttt ctaccgcata agaagctatt tcccctgttc taggcgaacg
     5281 caactcaaag gtcttaagct ttttagccca agaaacatcg ccagagaaaa caagaatata
     5341 gagctcacct ctaagtccat gggcatcttt aacttttcca accaatttca tttccatagc
     5401 gaccttaatg ccacgcctta tctatactcg caatacgtgc tcgcaatatt tgaaattatt
     5461 ttcgaaatat tttttaaata aaaaaagggc ctttaagccc ttttcaaaaa tcaaaccttg
     5521 ttgcagaaac tagatgctac tcaacgatat caaggtggta acgtttattc agcttagtac
     5581 cggccgcacg aatgatggtt ctcattgccg ctgcagtttt cccctgcttg ccaataacct
     5641 tacccaaatc ttctttatca acctttaaag ctaataaagt tgtttgttgt ccagggatct
     5701 cgtcaacttc aacgtgctct ggcttatcaa ccaaagattt cgccataaat tcaacgaggt
     5761 cttttagatt ttccatgagt tcctccacat catgtgtccg caataacgac ccaagccccc
     5821 taataggact tggctatttt ttaattattt tgcagcttga acgagcttaa ctacatgctt
     5881 tacgcgctca gaaggctgtg cgcctttttt aatccactcg ttgtactttt ctaagtcaat
     5941 tttgactttt tgatcagcac cttttggagt tgggatataa gtaccaataa cttccaaatg
     6001 tttaccagta acatagcggc gagagtctgc cactgtgata cggtattttg gggtgtgttt
     6061 tgtacctgta cgagccaaac gaataacaac tgccatgaga atcctcttta tcaacttttt
     6121 aaacagctaa atatttagca aacttttgtg aaaaaaacaa taataagtcg actggtctac
     6181 cttttttgta aaaggagatc aagtttaaaa tgggaacttc attcccccac caccaccacg
     6241 acccattccc attttcatca acccactcat catctttttc gcatcttcga attgcttaat
     6301 caatttatta acgtcttgaa ccttcgttcc gcttccattt gcaatccttt gacgacgcga
     6361 agcattcaaa attttgtgat tttttctttc ttgcggagtc atcgaacgaa taatcgactc
     6421 taatttctta atttcttgat ccggaggagt catatttttg agttgctttg aaagatcacc
     6481 catccccggt aaaaacttca aaagactctc catgccgcct aattttttta attgctgaat
     6541 ctgcgctaag aaatcttcga ggtcgaattc attctttgcc atctttcgcg cgctctcgcg
     6601 agccgacttc tcatcgatca cctcttgggc tctttcaaca agcgttaaaa cgtctcccat
     6661 gtccagaaga cgagaagcca aacggtctgg atggaagatt tcaagcgcag caaccttctc
     6721 cccaacgcca aggaatttaa taggaatgcc ggtgacttca cgaatgctga gagccgcacc
     6781 tccacgggca tctccgtcta ccttagttaa aaccaaacca gttaagccca atctttggtg
     6841 aaaaccttca gcaacgttta cagactgttg acctaacatc gcatctgcca ccagaagaac
     6901 ttcctgaggg ctccagacct gtttgagatt ctgcagttcc gacattaatt gatcatcaat
     6961 ttgcaagcgt
//
LOCUS       DATBTE010000339         6437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_591607, whole genome shotgun sequence.
ACCESSION   DATBTE010000339 DATBTE010000000
VERSION     DATBTE010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591607"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VIG33_12650"
     CDS             <1..578
                     /locus_tag="VIG33_12650"
                     /inference="COORDINATES: protein motif:HMM:NF023352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3443 family protein"
                     /protein_id="HEY8271732.1"
                     /translation="PTAQQVSNPVTFLPSHNNGVILQLPKIPSAGSTSASGFVILGIG
                     TATNNTPISAKVFPANGNGYIQTNFNGTDYPASFIDSGSNGLFFPAPPNLPGCADSSG
                     AKGFFCPVTPLTLSATQAGKTGSPKVLVSFEIQNSAYAIISSPNFMFNNIAGNFADGF
                     DWGLPFYFGRTIFHGINGKSSVLGTGPYWAW"
     gene            734..2059
                     /locus_tag="VIG33_12655"
     CDS             734..2059
                     /locus_tag="VIG33_12655"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8271733.1"
                     /translation="MIKNRHLMTTLLMCLTLLSATSRAADEEPILQEGTFKGSKDAYP
                     DRDILFKIAVSLCTELNPQKTVSSLKISPLIQHYLSKAPDSTKTTYDSSGWTVDLFFP
                     KPSPGFLRLLKGDSFFMLDYRQNEKTIESVANISLGRDCRLSEARFTYYNASLRPMLR
                     VKFNSSYQQIQSLVMEEAMKEVPQKNPPTKKVVRIGIIDTGIDYNHPALISKSRPMLG
                     LDLITKGRPPYDYTNSIQNEQMGKHFSHGTAVADIATRNIDALIIPVRTSNQPSLDGA
                     AVEYLAKKDVRIVNISQGTSDKSEWLPLKTAMQKHPEILFIVSAGNTAQDIDKEPFYP
                     SEFNLPNMLAVASVNTQGQLSEFSNYGAKHVHLAAIGENVSAALAGGGQWTVNGTSFA
                     APVVTNIAAQLLIEDPSLKSQELRSLLIQLAKPVEGLKGKVQYGVLGME"
     gene            complement(2089..2259)
                     /locus_tag="VIG33_12660"
     CDS             complement(2089..2259)
                     /locus_tag="VIG33_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271734.1"
                     /translation="MEALETEVPEIYGKLVLKYRPTSFVVVISGSTNAREICFQGKEQ
                     DEAVAFIQNLIF"
     gene            2570..2995
                     /gene="ndk"
                     /locus_tag="VIG33_12665"
     CDS             2570..2995
                     /gene="ndk"
                     /locus_tag="VIG33_12665"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY8271735.1"
                     /translation="MAVEQTFSIIKPNAMKKNAIGDIVSMFEANGLKIAAAKIQILSK
                     SKAEEFYAEHKARPFFGELVSFMTSGPVMLMCLQGEGAVLKNREIMGATDPKKATAGT
                     IRAKFGDNVGENAVHGSDSVESAARELTIFFEKQEICNV"
     gene            3010..4191
                     /locus_tag="VIG33_12670"
     CDS             3010..4191
                     /locus_tag="VIG33_12670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEY8271736.1"
                     /translation="MSSLVNKQLTVTIEKLAIGGSGIARHDGFVIFVPNSAPGDELLI
                     EVTTKKKNFAEARILEILKSSPSRRTPPCPIADRCGGCNWQHITEDEQRKQKQTMVQE
                     ILVKFLPGHELPFLPIKESPLSLRYRNRIQPKYFQGHLGFFARRSHGIIEAQDCPITE
                     EKLTAEFGKIKSELDKKFLTSKEIQRLELYLDRNEHPQWLLLGEEKDGVGFSQVNRFQ
                     NEDLIKTVLEWSHGSEYEHIFDLYAGSGNFTFPLSEIFSTQQINAVELDSKLVQQARK
                     KIQLSTSLNKRIHFHQSDVANFLQGAKLRSKDLVVLDPPRAGADEVVMKSLAQGTVAR
                     IIYISCHPASLARDLKYFFATQEHLPIVNRLKLKKIQCFEMFPQTDHVETIAELAVDT
                     P"
     gene            4213..4968
                     /locus_tag="VIG33_12675"
     CDS             4213..4968
                     /locus_tag="VIG33_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8271737.1"
                     /translation="MAKIILIFSIFIFAACASEKFKDSEKAELFLRMGSSQFENGNYP
                     AALNDLLKAEELDPKNPAVQNNLGLVYFMRQRYDLSEKHIRRALSLNNKYTEARNNLS
                     RVLIEEGKFSEAEKELRLVIDDLTYPSVDKAYINLGLAQFNQKNYTQAKESFLHAINF
                     SRNNCVANAYYGRSFFELKDYSSASGALDTAIGFCQRSLYDEPHYYSALTYYRLGNKE
                     KAMARFTEIVNLYPEGKYREKSRAMLEMLRKAQ"
     gene            4968..5954
                     /locus_tag="VIG33_12680"
     CDS             4968..5954
                     /locus_tag="VIG33_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8271738.1"
                     /translation="MKRTGEILKKAREQKGLSINEIALSLKISSKVLKAIEDDDSSQL
                     PAKTFLRGFVQSYASYLRLDVNEVLRIFQEERGSTKPNPLIQMPEQDATLESFEKKNT
                     ETSHPRGPSVSQEKSFKNLTKTTDNSKTLTFTILGLVLVGLIVFTKKMIDKYQKEGVV
                     SELEVTTPLTTQANEISSDATQDATSPTESTPLTTQTSFTAPTPTTSPKPSLTPTPTL
                     TPTPTSTPSPSPTPTVTPTSNTSPSPSPTPNVVNKQIEVIVEALDNIEITYVTKEGQT
                     EKLTLSADQVHTFKSKNGIKLNVNNGGAINVIVNGKNLGIPGNLGKPIKLNF"
     gene            5956..>6437
                     /locus_tag="VIG33_12685"
     CDS             5956..>6437
                     /locus_tag="VIG33_12685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY8271739.1"
                     /translation="MRQFLQIWTFSLILKIGLAALLPLCSDETYYWIWSHHLQLSYFD
                     HPPFIAWLFYLGHWLEPYGQLLRLPIVLTGHLTFLVWFNILKTQFSWEKYKYWYALAF
                     FSPLVGFGSMIGTPDVPLLLFWSLSIYYFQQCLLHQKSYDYFLLGASLGLGFCSKYHI
                     V"
BASE COUNT         1883 a         1492 c         1256 g         1806 t
ORIGIN      
        1 ttcccactgc tcagcaggtt tctaatccag tgacattctt gcccagtcat aataatgggg
       61 tgattttgca acttccgaaa attccttcag cgggttcgac atcagcttcc ggatttgtga
      121 ttttggggat cggtactgca actaataaca ctcctattag tgcaaaagtc ttcccagcca
      181 acggcaatgg ctatatccaa acaaatttta acggcactga ttatcccgct tcttttatcg
      241 acagtggatc caacggactc ttttttccag ctcccccaaa cctccccggc tgcgctgata
      301 gtagtggagc caaaggcttc ttttgccctg tcacaccctt gactctctcc gcgacccagg
      361 cgggaaagac aggttcgcca aaagttctcg tctcttttga aattcaaaac tcagcttatg
      421 ctataatctc aagtcccaat tttatgttta acaatatcgc cggtaacttc gccgatggct
      481 ttgactgggg cctgccattt tactttggca gaaccatttt ccatggcatc aatggaaaat
      541 cttccgtatt aggtactggc ccttactggg catggtaagc ttctttttga attcaagaat
      601 gacggcttct gtgcctgctt ggacctattc tatttctcat cacatgctcc tgttttaccg
      661 accacttatc aactcaaggc ttactaattg caagctagaa gcttttcatt ttataaggaa
      721 ttagaggctt ctcatgatta agaatcgaca tttaatgact acgctgctca tgtgtttgac
      781 actcctttcg gcaacttcca gagccgcaga cgaggagccc attcttcaag agggaacctt
      841 taaagggtca aaagatgcct atcccgaccg tgacattctg tttaaaatag cagtgagcct
      901 ctgtactgaa ttaaatcccc aaaagactgt gtcttcattg aaaatctcac cgcttattca
      961 acattatctt tccaaagccc ctgattccac gaaaactacg tacgatagct cgggctggac
     1021 tgtagatctt ttttttccaa agccttcccc tggttttctg cgtcttttaa aaggcgactc
     1081 ttttttcatg ctcgattacc gacaaaatga aaagacaatt gaatctgtcg caaatatttc
     1141 tctaggaagg gattgtcgac tgagcgaggc ccgctttaca tactataatg cttctttaag
     1201 acccatgctg agagtcaaat tcaattcatc ctatcagcaa attcaatcct tagtaatgga
     1261 agaggcgatg aaggaagtcc ctcagaagaa tcctcctaca aaaaaagtcg ttcgtatcgg
     1321 tatcattgat acaggaatcg actacaatca ccctgctttg ataagtaaat cccgcccaat
     1381 gttgggattg gatctgatca ctaaaggccg tcccccttac gattacacaa atagtattca
     1441 aaatgaacag atgggaaagc acttcagtca cggaactgca gtggcggaca ttgctactcg
     1501 caacatagac gccttaatca taccggtacg cactagcaat caaccttctt tggatggagc
     1561 agctgtcgag tatctagcaa aaaaagatgt tcgaattgtt aatataagcc aaggcacttc
     1621 agataaatcc gagtggcttc ctttgaagac agctatgcaa aaacaccctg aaatactttt
     1681 catagtctcc gctgggaaca cggctcaaga catagataaa gaaccttttt accccagtga
     1741 gtttaatctt cctaatatgc tggctgtagc ttctgtaaat acgcaaggac aactctctga
     1801 gttctcaaat tacggagcta agcacgtcca tctcgcggcg attggtgaaa acgtctctgc
     1861 cgcgcttgct ggcggtgggc agtggacggt caatggaacc tcttttgccg cacctgtggt
     1921 taccaacatc gctgcgcaac tgctgatcga agacccttcg ctgaaatcac aagaattgcg
     1981 ttccctcctc attcagttag caaaacctgt cgaagggtta aaaggcaaag ttcagtacgg
     2041 agtactgggg atggaatgat tgtgaatgag gaaacccgca tcttactctt aaaaaatgag
     2101 gttttgaata aatgctacgg cttcatcctg ctcttttcct tgaaagcaaa tttcccttgc
     2161 attcgtgctg ccgcttatga caaccacaaa actcgtagga cgatatttta ataccaactt
     2221 cccgtaaatc tcaggaactt ctgtttccaa tgcctccata agttcccata taccctttgt
     2281 atcgtaccta aggctataag cctgggaggt ggctttcgaa aagtcttcgg ttgaaagctc
     2341 ttttagaaga actccgtaga attcaaactc atttcgagct acgtattggt ttgcaatcgt
     2401 atcgctctat cggctctcaa acctaatttt tataccaggc ttgggtctaa tcccgaatcc
     2461 ccatcgagaa cctctggaaa gtcaggaaaa ccgatgaatt tacgtggaca cgagagggtt
     2521 atttggttaa aacccaggaa ctcattcaat ataacacaag gggttaacca tggctgttga
     2581 gcaaacattc tcaatcatta aaccaaacgc aatgaaaaag aacgctatcg gcgatatcgt
     2641 tagcatgttt gaagcaaacg gtttgaaaat cgctgctgcg aaaatccaaa tcctatctaa
     2701 atccaaagct gaagagttct acgctgaaca caaagcgcgc cctttctttg gtgagttggt
     2761 aagctttatg acttctggcc cggtcatgct tatgtgcctt caaggcgaag gtgctgtttt
     2821 gaaaaatcga gaaatcatgg gcgcaactga ccctaaaaaa gccactgcgg gaaccatccg
     2881 cgctaaattt ggtgataatg ttggggaaaa tgcggtccac ggttctgaca gcgttgagtc
     2941 tgcagctcgt gaacttacaa ttttctttga aaaacaagaa atctgtaatg tttaatttat
     3001 ttgaagaaaa tgtcgtcgct cgttaataaa caactaacag ttacgattga aaaactagcc
     3061 atcggcggct cgggcattgc ccgccacgat ggcttcgtta tttttgtccc caactccgcc
     3121 cctggagacg aacttctaat agaagtcact actaagaaaa aaaacttcgc agaagcacgg
     3181 atcctggaaa ttttgaaatc tagcccctcg cgccgaactc caccttgtcc gattgccgat
     3241 cgttgtggcg gctgtaattg gcaacacatt acagaggacg agcagcgcaa acaaaagcaa
     3301 accatggtcc aagagatact tgtgaagttc ttgcctggcc atgaactgcc gtttctaccc
     3361 attaaagaga gtcccttatc cctacgttat cgcaatcgta ttcagcctaa atattttcaa
     3421 ggtcatttgg ggtttttcgc ccgccggagc catggaatta ttgaagctca agattgcccc
     3481 atcactgaag aaaaattgac ggcagaattt ggaaaaatta aatccgaact tgataagaag
     3541 tttttaactt caaaagaaat tcagcgctta gagctttacc tcgatcgtaa tgaacatcct
     3601 caatggcttt tattaggaga agaaaaggat ggcgtcggat tttctcaggt aaaccgcttt
     3661 caaaatgagg accttattaa gaccgtgcta gagtggagcc atggctcaga atatgagcat
     3721 atttttgacc tctatgcggg cagtggtaat tttacgtttc ctctttcaga gatattctct
     3781 acccaacaaa tcaatgctgt agaactcgac tcaaagctgg ttcaacaggc gcgtaaaaaa
     3841 atccaacttt ccacctcact caacaaacga atacattttc atcaatctga tgttgcgaac
     3901 tttttgcagg gtgcaaaact ccggtctaaa gacttggttg ttttagatcc tcctcgcgca
     3961 ggagctgacg aggtagtgat gaaatccctg gcgcaaggta ctgtcgcaag aattatctac
     4021 attagttgcc atccagcttc tctggctaga gatctaaaat actttttcgc cactcaagag
     4081 caccttccga tcgtgaacag gttgaagtta aagaaaattc aatgctttga aatgttcccc
     4141 caaactgatc acgtcgagac catagcagag ctagccgttg acactcctta agtgagtgaa
     4201 taacttggat gtatggccaa aataatcctg atcttctcta tatttatctt tgccgcctgc
     4261 gctagcgaaa aattcaaaga ttcggaaaag gccgaattat ttttacgcat gggcagttca
     4321 caatttgaaa acggaaatta ccctgcggca ttgaatgatt tacttaaggc cgaagagttg
     4381 gaccctaaga atcccgcggt gcaaaataat ttaggcctcg tctactttat gcgtcagcgc
     4441 tatgatttat ctgaaaaaca catccgtcgc gccctatctc taaataataa atacactgaa
     4501 gcccgcaata atttatcgcg tgtgttaatt gaagaaggca agttctcaga agcggaaaaa
     4561 gaacttcggt tggtaattga tgaccttact tatccaagcg tggataaagc ttacatcaat
     4621 ttaggccttg ctcaattcaa tcaaaaaaat tacacccaag ccaaagaaag ttttttgcac
     4681 gccattaatt tttctcgaaa taactgtgta gcaaacgctt actatggccg ttcttttttt
     4741 gaattgaaag attatagtag tgcttcggga gcactcgata ccgccattgg tttttgccaa
     4801 cgctcacttt atgacgaacc ccactattac agtgctctta cttactatcg tctaggaaat
     4861 aaagaaaagg ctatggctcg ttttactgaa atcgtgaatt tataccctga aggtaaatac
     4921 cgagaaaaat ctagggctat gttagaaatg ctacggaagg ctcagtaatg aaacgcactg
     4981 gagaaatcct aaaaaaagct agagaacaaa aaggactctc gatcaatgag atcgccctct
     5041 ccttaaagat tagcagtaag gttttaaaag ccattgagga tgacgactct agccagctgc
     5101 ctgcaaaaac atttcttcgt ggctttgttc aaagctatgc gagttacctg cgcctggacg
     5161 ttaatgaagt tctccgtatt tttcaagagg agaggggctc aacaaaacca aatcctctta
     5221 tacaaatgcc tgaacaggat gccactctgg agtcttttga aaaaaagaac acagaaacct
     5281 cacaccctcg cggcccctca gtctcccaag aaaaatcatt taaaaatctt acaaaaacaa
     5341 ctgataattc taaaactttg acttttacca tcctaggatt ggttctagtc ggcctcattg
     5401 tttttacaaa aaaaatgatc gataaatacc aaaaagaagg tgtggtctct gaacttgagg
     5461 taaccacccc gctcacaacc caggcgaatg aaatctcttc agatgcaact caggacgcga
     5521 cttcaccaac agaaagcacg cctttaacca ctcaaacttc tttcaccgct ccgacgccaa
     5581 cgacatcgcc gaaacctagc ttaacgccca ctccgacttt aacgccgaca ccgacatcaa
     5641 cgccgtctcc ctccccaact cccacggtaa ccccgacgtc aaatacttca ccttcgccca
     5701 gtccaacgcc aaatgtggta aacaagcaaa tcgaagtcat tgtagaagca ttggacaata
     5761 ttgaaatcac ctacgttaca aaagaaggcc aaaccgaaaa acttacctta agcgcggacc
     5821 aagtacatac ttttaaaagt aaaaatggga tcaaacttaa tgttaacaat ggcggagcta
     5881 ttaatgtgat tgtaaacgga aaaaatctcg gtattccagg aaatcttggc aaaccaatta
     5941 agttaaattt ttaaagtgcg acaatttcta caaatttgga ccttcagttt aatactcaaa
     6001 attgggctcg cggctcttct tccattgtgt tcagacgaaa cttattattg gatttggtcg
     6061 caccatctcc agcttagtta ttttgatcat cctcctttta tcgcttggct tttttaccta
     6121 gggcactggc ttgagcctta tggccaattg ctccgtttac ccatagtttt aaccgggcat
     6181 ctcacttttc ttgtgtggtt taatatattg aaaacgcagt tttcttggga gaaatataaa
     6241 tattggtatg ctcttgcttt tttttcaccc ttagtgggat tcggatctat gataggaact
     6301 ccggatgttc cattgctttt attttggtct ttatctatct attattttca acaatgtctg
     6361 ctgcaccaaa agtcatatga ctatttttta ttgggtgcct cattaggatt aggtttctgt
     6421 agcaaatatc atatcgt
//
LOCUS       DATBTE010000340         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_181780, whole genome shotgun sequence.
ACCESSION   DATBTE010000340 DATBTE010000000
VERSION     DATBTE010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181780"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VIG33_12690"
     CDS             <1..271
                     /locus_tag="VIG33_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271740.1"
                     /translation="VDLVSYNCSTAAMDALKGKCALKFKQNENGKELDLTASPEVGAP
                     WGNGYGSSDSRPMSRLTIVVTAKGEVLTVKDESGNRISTKFDFTR"
     gene            complement(493..912)
                     /locus_tag="VIG33_12695"
     CDS             complement(493..912)
                     /locus_tag="VIG33_12695"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEY8271741.1"
                     /translation="MNKVTNTVKEWFANANEDLRVAQALWTLGPEKYLKTIPYHCQQT
                     AEKSIKGYLAYKEIKFPKIHDIAILVNLMGPTASELSQLLLKADDLTDYAVAFRYPDA
                     SRKNEATVKDSEEALELAKKVYIAMSALIPFDSQWNL"
     gene            complement(909..1229)
                     /locus_tag="VIG33_12700"
     CDS             complement(909..1229)
                     /locus_tag="VIG33_12700"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY8271742.1"
                     /translation="MHVELEKDKNFAEIVSKLKAHFKPEKIFLFGSRAKGTSTPDSDY
                     DLLLVVKNSDKTLRERMREARQVLRGRTVAVDIFIYTSVEFDELKEEFSSIAYTAFTE
                     GLEL"
     gene            complement(1328..>1789)
                     /locus_tag="VIG33_12705"
     CDS             complement(1328..>1789)
                     /locus_tag="VIG33_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY8271743.1"
                     /translation="FSRFARSTTHLLQGLEEMKKTNTGFVSLTEKIDTNSPIGKAVFV
                     IISAIAALERDLIAERVKNGLANARAKGKLIGRKKLRDSDLIRKLLKAGMTYRAISAV
                     ANCSHGSVHQEKLAMKRDEALALKRKLEQEELEKAQMAPTVQFPEQSTEAA"
BASE COUNT          474 a          382 c          383 g          550 t
ORIGIN      
        1 tgtggatttg gtcagctata attgctccac tgctgccatg gatgcgctaa aaggaaagtg
       61 cgctctcaag tttaagcaga atgagaacgg gaaggaatta gatctgactg cgagcccaga
      121 agttggtgct ccgtggggga acggatacgg tagttctgat tctcgtccaa tgagccgatt
      181 gactattgtt gttacagcaa aaggagaggt tctgacggtt aaggatgaat cgggcaatcg
      241 tatttccaca aaatttgatt tcacgcgata gttctcttca tttagctggt ctgaagctac
      301 gattacgaag cttcagaaac actaaactct ttgtctagag ccgaagatga atccatcgaa
      361 aaccgagcca agaagttctt cttggcttat ttttcaaagt cttttgttca gagcgtattc
      421 actcaagatt cgcggtgcta ccgacctggc taagaaacag ggttttcggc tcatggatag
      481 atcactttag gcttaaaggt tccactggct gtcaaaagga ataagcgccg acattgcgat
      541 gtagactttt tttgcaagtt caagagcttc ttcagaatcc ttaacggtgg cttcgttttt
      601 tcgagaggca tctggatatc tgaacgcgac agcatagtca gtgagatcat ctgcttttag
      661 caaaagttga gaaagctcag aagctgttgg ccccataaga ttaaccaaga ttgcgatatc
      721 gtgtatcttt ggaaatttta tttccttata ggctagatat cctttgatgg atttttcagc
      781 ggtttgttgg caatggtagg gaatagtttt taaatacttt tcagggccaa gtgtccacag
      841 tgcttgagcc acccgcaggt cttcattggc attcgcaaac cattctttga cggtatttgt
      901 gaccttattc aaagctcaag cccttcagta aacgccgtat aagcaatgga actgaactct
      961 tctttgagtt cgtcaaactc aacacttgta taaataaaaa tatcaacggc gacagtacgt
     1021 cctcgcagta cctgccgggc ttcacgcatt cgctctctta aggttttatc tgagtttttc
     1081 acaaccaaca gtaaatcata atcactatct ggagtcgagg tgccttttgc acgagagcca
     1141 aaaagaaata tcttctcggg cttaaagtga gctttaagct ttgagacgat ctctgcgaaa
     1201 tttttatctt tttctaattc tacgtgcata caaactttcc gttaaagtta gtatgattta
     1261 gaaagaggtt ttatttctta gttaaaaatt tctagaaaag aaaattctta attaaaaaat
     1321 cgaggcctta tgccgcctct gtggattgct cggggaattg caccgttgga gccatctgag
     1381 ctttttcaag ctcttcttgt tccaacttcc ttttaagagc cagagcttca tctcttttca
     1441 tcgcaagctt ttcttgatga acacttccat gagaacaatt tgccactgca gaaatcgctc
     1501 gataggtcat gcccgctttt aaaagtttac gaatcaaatc tgaatctctt agcttctttc
     1561 tcccgatcaa tttacctttg gctctcgcgt ttgcaagtcc gtttttcact cgctcagcga
     1621 tcaggtcacg ttcgagggct gcaatggccg agataatcac gaacactgct ttcccaatgg
     1681 gagagttcgt gtcgatcttc tcggtcaagg aaacaaagcc cgtattggtc ttcttcattt
     1741 cctcaaggcc ctgaagtaaa tgcgtcgtgc ttctcgcaaa tctcgagaa
//
LOCUS       DATBTE010000341         1561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_114253, whole genome shotgun sequence.
ACCESSION   DATBTE010000341 DATBTE010000000
VERSION     DATBTE010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114253"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..>1561)
                     /locus_tag="VIG33_12710"
     CDS             complement(139..>1561)
                     /locus_tag="VIG33_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271744.1"
                     /translation="LLGFDSESCKRYVTRRLQGYEASPTLIQKAMNQIAKVRIRDDED
                     RIIPFLADISTTVIEDELREGTDQGFDVFDDSTPYPSNNDVTDHIVYSILRREQTRHD
                     LEIPICEVVELISDLVVDYGKRWPLEEMQDRLKIYYESRAQSILTKLLLNPLLVAQNE
                     DIELRYSFLGSYFEVLLMLRGLLKSSVERGFIRALSRLNRESEEARDLKRYFQAHISE
                     VEDAIRSIIPKLRSIAVTCETTMTTRGDRENAKGAISGLLTLFFGTQKLAIDTTSSKL
                     FSFYGVPEYTNSSRTLEGLFIRGTFPPVDFTNLIITNSKFQDYKNLWACRFKNTKFMY
                     SVFEDCFDVSCANTQLDSSMIDGTCDLGDLREAFAMVKTTKADEQAMIYNEVNRFLHS
                     FFRGDRFVDNKIDYIKFSNKVPGLAFQNFDRLVANGYFKLTKEKTVASFYEVAQDFKA
                     SVRKFLTDGYPDGKMKKFVALVR"
BASE COUNT          425 a          343 c          348 g          445 t
ORIGIN      
        1 cgatattgga aagtggtgag ttattgctga aaattaactg tctctttact gctcaggctg
       61 tgtaaaacgc acgaaaagtt ttcagctatt tcagtatctc tatacccctt aattgtatga
      121 gcgccgggca tggccacttc atcgcaccaa ggctacgaac tttttcatct tgccatcagg
      181 atacccgtct gttaaaaact tgcgcacact agctttgaaa tcttgagcaa cttcgtaaaa
      241 actggccacg gttttttctt tggtgagttt gaaatatcca tttgctacca ggcggtcgaa
      301 attctgaaag gcaagacctg gaaccttgtt agagaactta atgtaatcta tcttgttgtc
      361 gacgaatcgg tcgcctcgga aaaatgaatg aaggaaacga ttcacctcat tgtaaatcat
      421 agcttgttca tcagccttgg tggttttgac cattgcaaag gcttctcgaa ggtcacctag
      481 atcacatgtc ccgtcaatca tcgaagaatc gagctgagta ttagcgcaag aaacgtcgaa
      541 gcagtcctca aatacggagt acatgaactt ggtgttcttg aatcgacatg cccatagatt
      601 tttgtagtcc tgaaatttag agttggtaat tatcaagttc gtgaagtcaa caggaggaaa
      661 tgtgccccta ataaacaagc cttctagagt acgacttgaa ttggtgtatt ctggaacacc
      721 gtagaacgag aacagcttgg atgacgtagt atcaatcgcc aatttttgtg tcccaaaaaa
      781 tagtgttagc agtcccgaaa tagctccttt agcattctcg cgatcccccc gagtggtcat
      841 tgttgtctca caggtaacag ctatagatcg aagcttgggg ataattgatc ggatagcatc
      901 ctcaacttct gaaatatgtg cttggaagta ccgctttagg tcgcgcgctt cttcactctc
      961 ccgattcaaa cgggagagtg cgcgtatgaa gccgcgctcc acgctgcttt ttagtagtcc
     1021 acgaagcatt aacaggactt caaaatacga gccaaggaat gaatatcgta attcgatgtc
     1081 ctcattctga gcgactagga gggggttcaa tagcaacttc gtcagaatgg actgggcacg
     1141 agactcgtaa tagattttta accgatcctg catttcctct agcggccaac gcttaccgta
     1201 gtcaacaact agatctgaaa tcagttcaac aacttcacat atcggtatct ctagatcatg
     1261 ccgagtctgt tcacgacgta ggatggagta gacgatgtga tcggtgacat cgttgtttga
     1321 aggatatggg gtcgaatcat caaatacatc gaaaccttga tctgtgccct ctctgagctc
     1381 gtcctctatt accgtggttg aaatgtcagc taagaaagga atgatccgat cttcatcgtc
     1441 gcggattcga actttcgcaa tttgattcat tgctttttga atgagagtcg ggctcgcttc
     1501 atatccctga aggcgtcgtg ttacatagcg tttgcaactc tctgagtcga aacccaatag
     1561 t
//
LOCUS       DATBTE010000342         1711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1480899, whole genome shotgun sequence.
ACCESSION   DATBTE010000342 DATBTE010000000
VERSION     DATBTE010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480899"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VIG33_12715"
     CDS             <1..234
                     /locus_tag="VIG33_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271745.1"
                     /translation="HSIRTLIWGQIAEGILPWETLSPRPSVTIGHRDQSSLKKSFCFE
                     NKKVHLLSSLMFNFFVEKKFNQGAKDGLLRSHH"
     gene            209..1525
                     /locus_tag="VIG33_12720"
     CDS             209..1525
                     /locus_tag="VIG33_12720"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8271746.1"
                     /translation="MDFSDLIIKALGLQEVVIEKVEPNPQDLSLKVFARQKKATCRCH
                     QCSSPILYIHQWKERTIKGPPLGAFLYVTIILYQLRGHCHICSDRVKSARVPFVHPLF
                     QNMTLALFLGLNSKTLWDLDQRRMKMMKPLMGLPENIDLCKMSADEVHFRTMPKLDSI
                     TKPEIKFVTNLVCTNAAKILSNAPGRDAKALAQCLKVLTPEQRSKIRTFAVDMHEPFM
                     STIRKLCPQAQICVDRFHLAERVNEAFDELRKAEFKKAKEANDTFQLGMLSPHRRFVL
                     MEREKKLKKSDLKMLDRLRELNKNILNGMILVEHFHRMLDKTNVIEFQKSLVLWYRLV
                     RESELSPFRKLARLIRKYRGYIEAYINSRLTTAKSEALNNKIKVLRRSGYGYTNQTSY
                     LNKILQRCGYLNSKYINTNGWFWSIPEDLAQTTPFYKPYLYPQLIE"
BASE COUNT          526 a          341 c          365 g          479 t
ORIGIN      
        1 catagcatta ggaccttaat ctgggggcaa atcgcagagg gcatcctgcc ctgggaaact
       61 ctgtctccca gaccctcagt tacgattggt catcgtgacc aatcgtcttt gaaaaaaagt
      121 ttttgttttg aaaataaaaa agtccatctt cttagctctt tgatgtttaa tttttttgtc
      181 gagaaaaaat ttaaccaagg agccaaggat ggacttctca gatctcatca ttaaagcatt
      241 gggacttcaa gaggttgtta ttgaaaaagt tgaacctaat cctcaagatc tcagcctcaa
      301 ggtttttgca cgacagaaaa aagcaacatg ccgctgccat caatgttcaa gtccgattct
      361 ttatattcac caatggaaag aacgaacgat caaagggcct ccattggggg cttttcttta
      421 tgtgacgatt attctttatc agttacgagg gcattgccat atttgtagtg accgagttaa
      481 atcagcaaga gttccctttg tgcatcccct ctttcagaac atgacgttgg ctctgttttt
      541 aggtcttaat tctaaaacac tttgggatct tgatcagcgt cgaatgaaaa tgatgaaacc
      601 cttgatgggg ttaccggaga atatagatct ctgcaagatg agtgcggatg aagtccattt
      661 tcgaaccatg ccgaaactgg atagtataac aaaaccagag ataaagtttg tcaccaactt
      721 ggtatgtacc aatgccgcaa agattttatc aaatgccccc ggacgggacg caaaagcctt
      781 agcccaatgc ctgaaagtcc tcacccctga gcaaagatct aagattcgta cctttgccgt
      841 tgatatgcac gagcccttta tgtccacgat acgaaagctc tgccctcagg cacagatttg
      901 tgtagatcgg tttcatttag cagaaagagt gaatgaggct tttgatgaac ttagaaaagc
      961 tgaatttaaa aaagccaaag aagccaatga tacttttcag ttaggaatgc tttcgcccca
     1021 tcgcagattt gttttaatgg agcgggaaaa aaaattaaag aaaagtgatt taaaaatgtt
     1081 agatagactt agagaactca ataaaaatat tctcaatgga atgatcttag tggagcattt
     1141 ccaccggatg cttgataaga cgaatgtcat tgaatttcaa aagtccttag tgttgtggta
     1201 ccgattggtt cgcgaatcag agctgagccc ttttagaaag cttgctcggc ttatcagaaa
     1261 atatcgagga tacattgaag cctacattaa ctcgcgcctt accacggcga aatctgaagc
     1321 tctcaacaac aaaattaaag tccttcgtag gtcaggatat ggatatacaa atcagacctc
     1381 gtacttgaat aaaatcttgc aaagatgtgg ctacctgaac tcaaagtata taaacaccaa
     1441 tgggtggttt tggagcattc cggaggactt ggcacaaact accccattct ataaacctta
     1501 tctttaccca caattaatcg agtgatctgg tgtgatataa ttggcgcatt tataggaggt
     1561 ggccaatggg ttgctctacg acaatttggg cggcaagtga gtttaaggat atcgatctcg
     1621 gagatatccg aaggaataag agatttgtta gtgttgtttc acaaatggca cagcaaccgg
     1681 agcattctct gtgtcgagcc aacagctcat g
//
LOCUS       DATBTE010000343         4024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2300273, whole genome shotgun sequence.
ACCESSION   DATBTE010000343 DATBTE010000000
VERSION     DATBTE010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2300273"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VIG33_12725"
     CDS             <1..195
                     /locus_tag="VIG33_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271747.1"
                     /translation="EDAEQWKLLKSLGCGTFQGYYFCKPVPVHELEVSMALSANFLSN
                     HLGINILSLIKPEQLYPADE"
     gene            278..583
                     /locus_tag="VIG33_12730"
     CDS             278..583
                     /locus_tag="VIG33_12730"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEY8271748.1"
                     /translation="MSFIAFARYMHQKEVEHYEKKFPKLKTDKAAEAFVDEADLTQYD
                     LSGMKPVSFEFQPKDKSITMRLPETLFEAIKKEADRSGIPYQRFIRQTLENALPLGK"
     gene            818..3436
                     /locus_tag="VIG33_12735"
     CDS             818..3436
                     /locus_tag="VIG33_12735"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEY8271749.1"
                     /translation="MKNIFCKLSDLKNEATVESYFIDRLLSDLGYADDDISLKESISE
                     HAVGKGSKKSLYKPDYVVKSSGIPTIVIDAKSPKENINDWELQCSSYCLEINKEFDYN
                     PVKYYLLSNGLKTALYQWDQKKPLLDLDFADFVVGNDSYEELVKIVSKSSISEVSETL
                     KEELELSSFRFEKVSLEELSAKFQKLHQEIWQSEKKGPSAAFQELIKIFFVKIRKDRD
                     IHLKLGKNPSPKYRDVVFSQNWIATQTETKNPINEILFRNLVSEFEKDILSGKKKRFF
                     EKDDQINISSSTMKKVAKEIENIDLFGMEEDVHGRMFETFLDATIRGKDIGQFFTPRD
                     VVDLMVEIGDPKASKNHVDTVLDACCGSGGFLIASLSKMLRQASGICGLSSKERIELE
                     EKIKTQSVYGIDAGSDPAMYKIARMNMYLHGDGGSNIYYADSLDKGIGRVGNENLEVD
                     KQIEELRTMVLDKGRKFDLILSNPPFSLQYTRENRDQSHVLNQYALSRDRVGGKIINK
                     LISSVMFIERYKDLVSDSGKIIAVIDDSVLSGSSYEYIREYIRNNFIIEAIISLPGDA
                     FRRAAARVKTSVIVLRKKSKDDSQDDIFMASSIYLGLEQKTAKRIGIKYAELDEKKIA
                     EKEAIVSAYRNYLSGKDSAYIIPFSNCIDRLDVKYCINDRGRKAPLWKKKGLKVEPLG
                     NILSLATKRDVKVEEDSQYPLLIVNYDGEINDGEILDGADSSYSKLFKVKTWDLLISN
                     MGFGRGAISVVPPHYNGKYVSNEYTILRAQTNECAVFYWNLLRTKEILGDVFSSTTGM
                     NRGRIKWDIIKTVLVPVYEENIEIKKLTDEIEEFWKAYAKFSKSKAVHVTRVSNELDV
                     AGEESFERWLSFKPPE"
     gene            complement(3637..>4024)
                     /locus_tag="VIG33_12740"
     CDS             complement(3637..>4024)
                     /locus_tag="VIG33_12740"
                     /inference="COORDINATES: protein motif:HMM:NF028498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HEY8271750.1"
                     /translation="AWVDWNMVLDETGGPNHVNNLCSAPIIADTQAGEILYQSSYYYI
                     GHFSRFIRPGARRVACAKTLDSLEASAFLNIDGTVAVVVLNRTEQAIDFILKSQGLQA
                     KTSIPARGIKTFFFNNCWASCLGPIF"
BASE COUNT         1300 a          717 c          896 g         1111 t
ORIGIN      
        1 gaagatgccg aacagtggaa acttctgaag agcttgggat gcggcacatt tcagggctac
       61 tatttttgca agccggtccc tgtccacgaa ctagaggttt ccatggcact gagtgcgaat
      121 tttctaagta atcatttggg tatcaatata ctgtcgctta ttaaacctga gcagctttac
      181 cccgcagacg aatagatttg ctctaatcag attgggcatt cccatttact ttttcggata
      241 acctacgctc tgcaccgcga caatcctttg cgttgaattg agcttcatcg ctttcgcccg
      301 ttacatgcat caaaaagagg tagaacatta tgaaaagaaa tttccaaaac ttaaaaccga
      361 taaagccgcc gaggcttttg tagacgaagc cgatttaacc caatatgact tgtcaggcat
      421 gaagcctgtt agttttgagt ttcagccaaa ggataagagc attaccatgc gcctgcctga
      481 aacgctcttt gaagctatca aaaaggaagc cgatcgttca ggcataccct atcaacggtt
      541 tatccgccag acgcttgaaa acgcattgcc ccttgggaaa taagccgtat ttgccattga
      601 tgagtcccgt cgggcgacag tttttcgttg cccgacattt tgatgtatgt gaatttcggg
      661 cataaatagc gataatgttg ggcagaaaaa cgttgcccaa cctacccggc tatttgcgag
      721 ccgaactacg taatcccaga attaaggctt attcgggagg gtagcgaaag tcctaacact
      781 attgaacatt agaaaacctg aagagagaac gaccgaattg aaaaacatat tttgtaaatt
      841 atccgatttg aaaaatgaag caaccgtaga gtcgtatttt atcgacaggc ttttatctga
      901 cttaggttat gctgacgacg acatttcact caaagagagt atatctgaac acgcagtagg
      961 aaaaggcagc aagaaatctc tctacaaacc agactatgtt gtgaagtcat caggtatccc
     1021 tactattgta atagatgcta agtcaccaaa agaaaacata aatgactggg agctacaatg
     1081 ttcatcttat tgcttggaaa tcaataaaga attcgattac aaccctgtaa agtactattt
     1141 attaagcaat ggcctgaaaa ctgccttata tcagtgggat cagaagaagc cgcttctgga
     1201 tctagacttt gcagattttg tggtaggaaa tgatagttat gaagaactcg ttaagatagt
     1261 ttcgaaaagt tcaatttcag aagttagcga aactctaaag gaggaacttg agctttctag
     1321 tttcagattt gaaaaggtgt ctcttgagga actttcggca aaatttcaaa aattgcatca
     1381 agagatttgg cagtctgaaa agaaaggtcc tagtgctgct tttcaagagc ttataaaaat
     1441 cttctttgtg aagatcagaa aagacagaga tattcacttg aaactaggaa aaaatcctag
     1501 tcccaaatat cgggacgtag ttttttctca gaactggatc gccacacaaa ctgaaacaaa
     1561 aaatccgata aatgaaatat tatttagaaa tctagtttct gaattcgaaa aagatattct
     1621 ttcaggtaag aaaaaaagat tctttgaaaa agacgatcag ataaatattt cttcttctac
     1681 tatgaaaaaa gtcgcgaagg aaatagaaaa cattgatcta tttgggatgg aggaagatgt
     1741 acacggccga atgtttgaaa ctttccttga tgcaaccata agaggaaaag atataggtca
     1801 gtttttcacc cctagagatg tagttgatct catggttgag attggcgatc caaaagcaag
     1861 caaaaatcat gttgatacag tgcttgatgc atgctgtggc tctggtggct ttttgatagc
     1921 gtcactatcc aaaatgctaa ggcaagcatc agggatatgc gggttatctt caaaggaacg
     1981 catagaacta gaagaaaaaa tcaaaactca aagtgtatat ggtattgatg caggaagtga
     2041 ccctgcaatg tacaaaattg caagaatgaa tatgtattta catggagatg gtggaagcaa
     2101 tatttattat gcggactctt tagataaggg tatagggcga gttggaaatg aaaatttgga
     2161 ggtagataag caaattgaag aactcaggac aatggttctc gataaaggca ggaaatttga
     2221 ccttattctt tcaaatcctc ctttctcatt gcaatataca agggaaaata gagaccaatc
     2281 ccatgtacta aatcaatatg cattgtctcg tgatagagtt ggtggaaaaa taattaataa
     2341 actgatcagc agcgtgatgt ttattgaaag atataaagat cttgtttctg actcgggaaa
     2401 gattattgca gtaattgatg attcggtgct ttctggatcg tcttacgagt atattagaga
     2461 atatataaga aataacttca ttattgaggc aatcatatcg cttccaggag acgcatttcg
     2521 acgtgctgcg gccagggtga aaacatctgt aattgttttg agaaagaaat cgaaggatga
     2581 ttctcaagat gatatattca tggcgtcatc aatttactta ggattagagc aaaaaaccgc
     2641 aaagcgtatc gggattaaat atgctgaact ggatgagaaa aaaatagccg agaaagaggc
     2701 tattgtttct gcttatcgga attatttaag tggaaaagat agcgcataca ttattccatt
     2761 ttccaactgt attgaccggc tagatgtaaa atactgcata aatgatagag ggagaaaagc
     2821 tccattatgg aagaagaaag ggttaaaggt ggagcctttg ggaaatattc tctcgttagc
     2881 gacaaaaaga gacgtaaaag tagaggaaga ttctcagtat cctttgctta tagtgaacta
     2941 tgatggagag ataaatgatg gtgagatact agatggagct gattcgtcgt attcaaaatt
     3001 gttcaaggtt aagacatggg atttattaat atctaatatg ggctttggtc gaggagctat
     3061 cagtgtagtt cctccacatt ataacggtaa atatgtttct aacgagtata cgatacttcg
     3121 cgcacaaaca aacgaatgcg cggtgtttta ttggaatttg cttaggacga aagagatatt
     3181 aggagatgtt ttttcatcaa ctacaggaat gaacagagga agaataaagt gggatattat
     3241 caaaacagtg ttggtccctg tatatgagga aaatattgaa attaagaaat tgacagatga
     3301 aattgaagaa ttttggaaag cttatgccaa attttccaaa agcaaagcgg ttcacgtaac
     3361 gcgtgtttcg aatgagttgg acgtggctgg agaggagtcg ttcgagcgct ggttatcatt
     3421 caagccccca gaataattcg ggctaacaat atgttgcact cggactgcgc tacgcgcatc
     3481 tggtgatcac aatgttaaag tgtagtgatt gaaaactcac tgccttcttt gggtcgaatt
     3541 cagccagtca atgccagatt ccattgtgga tacttttgaa gccgtcagtc ccgtaaggtc
     3601 gagcaacaga ttttagttgc ccgacattca tatacatcaa aatatcgggc ctaaacaact
     3661 tgcccagcaa ttattaaaaa agaaagtctt gattccacga gccggtattg aggttttagc
     3721 ctgcaatccc tggctcttca gaatgaagtc aattgcctgc tcggtgcggt tcaagaccac
     3781 tacagccaca gtcccatcaa tattcaaaaa tgcgcttgct tcaaggctgt ccagggtttt
     3841 ggcgcaggcc actcggcgag cccccggccg gatgaagcgg gagaaatggc caatgtagta
     3901 ataagagctt tgatagagta tttctcccgc ttgggtgtcg gcaataatgg gcgcgctgca
     3961 taaattgttc acatggttgg ggccgcctgt ttcatccaga accatgttcc agtccaccca
     4021 tgcc
//
LOCUS       DATBTE010000344         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_456708, whole genome shotgun sequence.
ACCESSION   DATBTE010000344 DATBTE010000000
VERSION     DATBTE010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456708"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VIG33_12745"
     CDS             complement(<1..580)
                     /locus_tag="VIG33_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271751.1"
                     /translation="MIFLKNSKIVGCVAIMSVLGCANRPQKVTELDKPRTSYTEEAES
                     RDITNEAAKKSKAHNFVEINFNPRSAVLSDDAKTSLKKIIEEASQAGRITEILVLSWA
                     DQDFPSEKIQQLPKDQRQLAEKRNTVIETYMNTLRSGKVDTYNMAEQANILSKWFKTS
                     DSQLKESLLEAGLPTTADSFRYPSKASHSVIFV"
     gene            1019..1273
                     /locus_tag="VIG33_12750"
     CDS             1019..1273
                     /locus_tag="VIG33_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY8271752.1"
                     /translation="MTINENAAKGKWLEIKGDIQKAWGNLTDDDLEKTKGDMKAVGGL
                     IQQKYGQAQESYSKKLSDIFARIESKKDAVVTDVKNNLKN"
     gene            complement(1557..>2144)
                     /locus_tag="VIG33_12755"
     CDS             complement(1557..>2144)
                     /locus_tag="VIG33_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY8271753.1"
                     /translation="PTLLMSQMALAETKVPEGNYKLDVSHSKVGFEVNHLVVSTVEGK
                     FSDFEGMINIDPKLEKSKVDITIKATSINTDNKDRDEHLRSPDFFDVKKYDKLTFKSK
                     KISGTPEALKVAGDLTLHGVTKPVVLDTKYTGTVKDPWGNERVAFKAATKISRKDFGL
                     TWSKAVEAGPVVGDEVTLDLKVEAIKEKPAAAATK"
BASE COUNT          631 a          457 c          425 g          631 t
ORIGIN      
        1 tgacaaaaat gacagaatga gaggccttgc taggatatct gaaagagtcg gcggtcgtgg
       61 gcaagcccgc ttccaaaaga gattctttga gctggctatc gctggtctta aaccactttg
      121 agagaatgtt agcttgttca gccatattat aagtatcaac ttttccactt cggagagtat
      181 tcatatacgt ttcaatgacc gtatttcttt tttcggccag ttgtcgttga tcttttggaa
      241 gctgttgtat cttttctgac ggaaaatctt gatcagccca agagagcaca agaatctccg
      301 taatccttcc agcttgactt gcttcttcta taattttttt caatgaagtt ttagcatcat
      361 ctgaaaggac tgcgcttcgc gggttaaagt taatttcgac aaaattatga gctttagatt
      421 ttttcgccgc ttcattcgtt atatctcgac tttctgcttc ttcggtatag gacgttctcg
      481 gcttatctaa ttctgttact ttttgcggcc gattcgcaca acccaggacc gacattatgg
      541 ccacacatcc cacaatcttg ctatttttca gaaaaatcat ttctttctcc ttgtttgttt
      601 tttgatggtt cagacgtgat aacaacttaa gttcgaagag cttataagca ataatctgac
      661 cactttgaga aggtgacaaa tagcctctac atttcgcaga cataagttga gatgtggaat
      721 ttcatgaaga agtagagttc aattaagtgg gaaagttacc ccgtataggg catatttccc
      781 tacaaaaaaa tcacactttt taccacacac cactcaatgg atctcaagaa tgaaaacact
      841 agggcatcaa atttgcattg cttgatctgt acgagagaag tccaaaatga aaaatagata
      901 gccctggagg aatatatgat acgcgcagca gtttcttttc tggtcgccat ggtgacagaa
      961 aaaaagccga tcccacttcc ataataaatt tagcaacact gcaacaaagg agtcagaaat
     1021 gacaatcaat gaaaacgcgg caaaaggcaa atggctagag attaaagggg acattcaaaa
     1081 agcttggggc aacctaactg atgatgacct tgagaaaaca aaaggggata tgaaagcggt
     1141 cggaggactt attcaacaaa agtatgggca agcccaagag agctacagta aaaagctttc
     1201 ggacattttc gccagaattg agtcgaaaaa agatgcagtc gtcacagacg ttaaaaataa
     1261 tttaaaaaac tgaattttct agtagaatct ggttcggaat atcgaagcga cgttggttta
     1321 ttgttggcga aaaatataaa aaaatccatc cgataaatca aaaaattcgg tattccgaaa
     1381 attctgatgg agacaggacc gcttccgcag gccaaatttt ttcgcgatcc cagagccgcg
     1441 agctcctcac cgaactgatt tttctcaatc gcattcaatt ggagaaccat gaccaacaaa
     1501 tatcacaggc attctgctgc tgttatgttt ttcgttggcc atcaatccct cctaaatcac
     1561 tttgtcgctg ctgcggcagg tttttctttg atcgcttcca ctttcaaatc caaagtgact
     1621 tcgtcaccaa cgacaggtcc tgcttcaacg gctttagacc aagttaaacc gaaatctttg
     1681 cggctgattt tagtcgcagc cttgaaagcc acgcgctcat tgccccaagg gtctttcaca
     1741 gttccggtgt atttcgtgtc cagcaccact ggcttggtca cgccatgaag ggttaaatca
     1801 cccgccactt ttaaagcctc aggagttcca gaaatctttt tggatttaaa ggtcaatttg
     1861 tcatattttt ttacgtcaaa aaagtcgggg cttcgcaaat gttcatcacg atctttattg
     1921 tcggtattta tggaagtggc tttaatggtg atatccactt ttgacttctc aagcttcgga
     1981 tcgatattta tcataccttc aaaatctgaa aactttcctt ccactgttga aaccactaag
     2041 tgattcactt cgaagccgac cttagaatgg gatacatcca acttatagtt cccctctgga
     2101 actttagttt cggccaaagc catttgagac atcaaaagcg ttgg
//
LOCUS       DATBTE010000345         2749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1413003, whole genome shotgun sequence.
ACCESSION   DATBTE010000345 DATBTE010000000
VERSION     DATBTE010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1413003"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            705..1361
                     /locus_tag="VIG33_12760"
     CDS             705..1361
                     /locus_tag="VIG33_12760"
                     /inference="COORDINATES: protein motif:HMM:NF016879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Coq4 family protein"
                     /protein_id="HEY8271754.1"
                     /translation="MSSSFSLKIMGRWAVFKEFIKVYREEKYLGDLATLRASMNLGSF
                     EPTIEVGAFLDSWNDVPDFSEIDFESMPVDSLGWAYNNFLKVNGLKPFRFSDRYLDLM
                     KQHKLVVRYASVHDIFHVLTGFDTSLAGEAGVWAFVAGQGFSPDAAKAAKLTQIIYPL
                     VSPTQRRWIRAAYTEGLRNGVGAKCLITVDFKSEFLRSVDDVRARYNVKPTQLAKFLV
                     "
     gene            complement(1620..2132)
                     /locus_tag="VIG33_12765"
     CDS             complement(1620..2132)
                     /locus_tag="VIG33_12765"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEY8271755.1"
                     /translation="MFEDEACFRQDSTLHRTWSRKGCQPLVPVTGQRKSVKVFGCVDI
                     VTAKFIYNMDEVFNVPSYLNFLEYAGTEMFQKNKRVFYIQDNASYHKSDETWKWFKEN
                     RKWIEVFNLPPYCPELNAAEPLWKYTRREGTHNVCFESEEQIHSTLHAVFDKVQSQPK
                     EITGYLRPFL"
     gene            complement(2132..2683)
                     /locus_tag="VIG33_12770"
     CDS             complement(2132..2683)
                     /locus_tag="VIG33_12770"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY8271756.1"
                     /translation="MSPRFIKLHPRTEKKLIRMERNASADGAYRVSTRIRAILLNHQE
                     KTSGEIATTLNAPRSRVSEWLKTYDEQGVDGLMEGQRTGRPSRLSDVQKIMLCDILDS
                     GPIAYGQLSGIWTAKIISKIIKDEFDVHYHSSHVWKLLQDFGFTVQSPKRLLAKADEE
                     KRQMWIKKTCPQIKKKPREKALA"
BASE COUNT          742 a          572 c          599 g          836 t
ORIGIN      
        1 catcggaata tggcgggatt ctcagaataa aacaggacca gatactggtg tcagttattc
       61 aatcacgaca atggtctcag ttaagtggcg agcacgaaga ctacgcgttg gatatccacc
      121 gccggaggcg gttcagtcca atggaatgaa tttcgacatg tattcaatct gacagaaaag
      181 gccttcccga gcttagcact agggacgggg gctattctta aatgccaaac tctttaggag
      241 caaggcaaat acattccgcc tacggtcgcc accgtatctt tatcttcgaa atccaatcat
      301 tcaaaaagac gaccatcagc aacaacaaaa ccccagcagc aagcgtcgac aatcttacac
      361 aagcggagat gggatcactt tgccgatttt aggaatataa ggaaacaggt agatgatccc
      421 caacccaaat gccgtcatta cgtagacatg ccatgtaaca ttcgtaagtt caggaagttg
      481 tgccataaat atcagaattg caagggtatt cacaaaaccc gttaccacgg attgtgagat
      541 gaaactcatc aacgacccga gttttaaata tccataaatc tgaaggcgtt aaagattttg
      601 aagcttcgtg cgggatgcct tagcccagcc ttttatgcag ccaatcggct cggattcatc
      661 tttggattta cgattgtagt tcttaagttc gagttttgac aaagatgagt tcttcattta
      721 gccttaaaat catgggaaga tgggcagtct ttaaggaatt tataaaagtt tatcgggaag
      781 aaaagtacct cggtgatcta gcgacacttc gggcctcgat gaatctgggt tcatttgaac
      841 ctacgattga ggtgggggca tttcttgatt cttggaatga tgtgcctgat ttttctgaaa
      901 tcgattttga atctatgccg gttgattcat tggggtgggc ctataataat tttcttaaag
      961 tgaatggact taaacctttc cgtttttcgg atcgctatct tgatttgatg aaacagcata
     1021 agcttgtagt tcgttatgcc agtgttcatg atatttttca tgttctaacc ggatttgata
     1081 cttcgctcgc aggcgaagct ggcgtctggg cttttgttgc aggtcaagga ttctctccag
     1141 acgcagcgaa ggccgcgaag ctgacacaga tcatttatcc actagtcagc ccaacgcagc
     1201 gaaggtggat tcgggcggcc tacactgaag gtcttcggaa tggagtgggc gccaagtgtt
     1261 taataacggt cgactttaaa agtgaatttt tgcgttcggt tgatgatgtt cgtgctcgtt
     1321 acaacgtaaa gcctactcag cttgcgaagt ttctagtgta aataaatagt taatgacagc
     1381 ccttggtcct aggggtaatc ttctaattca tgcgaataaa aagggttttg cgcggttaat
     1441 gcgtaagagt atttcgatgg tagggtcgcc atacttttga gaggatgccc tatgtttaat
     1501 cgaacaaaag tttatcttct tggttttata tttttcacat ctcaatatgc catggctcaa
     1561 attgatggaa gattaaaatc caagctatat agaattgcat aattaaaagg acataatgat
     1621 cataaaaatg ggcgcagata gcccgtgatt tccttgggct gactttgaac tttatcgaac
     1681 acagcgtgca aagtggaatg gatctgttct tcgctttcaa agcaaacgtt gtgtgttcct
     1741 tcgcggcgtg tatacttcca caaaggctca gcagcattga gttctgggca gtacggcggc
     1801 agattaaata cttcaatcca ttttctattt tctttgaacc atttccaggt ctcatcactt
     1861 ttatgataag aagcattgtc ttggatgtag aaaactcgct tgtttttctg aaacatttca
     1921 gtccccgcat attccaggaa attcaagtag gacgggacgt tgaagacttc atccatgttg
     1981 taaataaatt ttgccgtcac aatgtcaacg cagccaaaaa ctttcacaga ttttctttgt
     2041 ccagtcaccg gcaccaatgg ttgacaccct tttcgtgacc aagttcgatg cagtgttgaa
     2101 tcttgcctaa agcaggcctc gtcttcaaag attaagcgag agccttctct ctgggctttt
     2161 ttttaatttg tggacaagtt ttctttatcc acatctgtct cttttcctcg tcagccttgg
     2221 caagtagccg ttttggactc tgaacagtga agccaaaatc ttgcagtagt ttccaaacat
     2281 gacttgagtg atagtgaaca tcgaactcgt ctttgatgat tttggaaatt atttttgcag
     2341 tccagatgcc actcaactgc ccgtatgcaa taggaccact atcaagaata tcacaaagca
     2401 taatcttttg aacatcactg agccgacttg gccgacctgt tcgttggccc tccattaatc
     2461 catcaactcc ttgctcatca taggttttaa gccattcaga aactcttgat cgcggggcgt
     2521 taagtgttgt tgcgatctcg ccgctggtct tctcttggtg attaagaagg attgctcgaa
     2581 tcctggttga aacccggtaa gccccgtcag cagaggcatt gcgttccata cgaataagtt
     2641 ttttctcagt tcttggatgt aatttgataa atcttggcga catcttgcga tcttaggtcg
     2701 acttttattt tacttcaact atgtcctttt aattatgcaa ttatatata
//
LOCUS       DATBTE010000346         9574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2300988, whole genome shotgun sequence.
ACCESSION   DATBTE010000346 DATBTE010000000
VERSION     DATBTE010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2300988"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VIG33_12775"
     CDS             complement(<1..951)
                     /locus_tag="VIG33_12775"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEY8271757.1"
                     /translation="MKKTIALIFGGRSSEHEVSIRSAKNVANALDKNKFNFLLVGISK
                     EGSWYQFSGMEVFDQIVQLTDKSLPASSDPVALISEAGKPWFFSLKKQTKTAVDCAFP
                     ILHGTFGEDGTIQGLFKMMNLPFVGCGVWSSAAGMDKEVMKRLLAVAGVPNAKYELLT
                     PHHRLTYEELVKSLGSPFFIKPANAGSSVGVHKIKAKETFEKDLKDAFNYDTKVLAEE
                     FIQGREIECSVMGLNATAQASLPGEVIAHHEFYSYDAKYTDEQGASIVIPAQLSTAEV
                     EKIQKMAVHTYQVMGCDGLTRVDFFLQKNGELCVNEINTIP"
     gene            complement(948..1601)
                     /gene="elbB"
                     /locus_tag="VIG33_12780"
     CDS             complement(948..1601)
                     /gene="elbB"
                     /locus_tag="VIG33_12780"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HEY8271758.1"
                     /translation="MRKIAVILSGCGNKDGAELTEAISLLISLSQNGAEYRCFAPNIE
                     FNALDFIKNEPLGTKRNALTEAARITRGEIQDLKNLEVSKYDALAFVGGYGAAKNLSN
                     WAEKGAQCTVLPEVEKAIIDFHAASKPIAAICIAPVLIAKVLGSEKVTVTVGDDKETI
                     AKILKTGAQHEICPVDDYVTDRETKVITTPAYMYDNAKPHEIFKGISALAKELVEMA"
     gene            complement(1676..2581)
                     /locus_tag="VIG33_12785"
     CDS             complement(1676..2581)
                     /locus_tag="VIG33_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271759.1"
                     /translation="MINTLFNALKRSLLLLALLAGTSCLKPPSLSDDNGPEVPVDQVQ
                     KAILEGWDNADFNSVRLNEFLYVEQDQKIATLDPKVVYKEATQVIARVENADTVDYKF
                     LIRSQAMDNGSFKPVTAFENDISIPKTVPTLTGTPALAEEKTAFPNSLETLQKSLANG
                     SVGLRAGGTQSQFLGVLTVRNMLGACVKAKDWDVTCHNLQVSEGKRPAPTGVASKPDC
                     GGIPNCEVNYKKVSFDLVVNLVDEKTNTTHPEKVNYEMTFSSDVPYLSRLLEFCYLGM
                     VPTATQKVIVKICNKVQSFEVGTTN"
     gene            complement(2578..3669)
                     /locus_tag="VIG33_12790"
     CDS             complement(2578..3669)
                     /locus_tag="VIG33_12790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8271760.1"
                     /translation="MEGPRSPAESELPTVLNFLNHSLRSHSTWSIAAEYPTAFSTTNL
                     HNMRIITDEDQVVSHAVLKPLIVKSPHLIFKVGSIGSVVTNETHRHQGLSTKIIQDCL
                     QQATRQQCDIAILWTDLYDFYRKMGFELAGTEISLVIEEEFAAPDPSLRLSKETKVAA
                     EAIHRLYSTHTVNSVRTIEETRKYLNIPQTQVYTAWDSSGQLAAYAIEGKGADLGGYI
                     HEWGGSVTAIITLLSYIRREKKQAITIIVPPHSQNLIQRLREKPVTYNQGYLGMIKIL
                     NFDQLAAKIKRAFRAEGISDIVLESRDGQYVFGCNGDLAVLGGETNLTHLLFGPVDFH
                     MLDVFKPETRTKLSKILPLNLWIWGWDSI"
     gene            3835..4074
                     /locus_tag="VIG33_12795"
     CDS             3835..4074
                     /locus_tag="VIG33_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEY8271761.1"
                     /translation="MAELDQKWKENKHGKMFVDQSCIACDACILTAPKNFTMHEEDGH
                     AFVSKQPETPEEEAACKEAMEGCPVEAIGDFGDKA"
     gene            4078..4713
                     /gene="nth"
                     /locus_tag="VIG33_12800"
     CDS             4078..4713
                     /gene="nth"
                     /locus_tag="VIG33_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEY8271762.1"
                     /translation="MKKAPANAAIVLKLLQRYYPDAHCALNHRNAFELLIATILSAQC
                     TDERVNIVTKELFAKYPSPEKMAKAAIEDLEHLIRSAGFYKNKAKNIKACSQSLVEKY
                     KGQVPETMEELVGLAGVGRKTANVVLGNAFAVASGIVVDTHVARLSYRLGWVESENAA
                     IIEKRLCEFVPKNHWIQLSHELIFHGRQICKARKPQCERCFLCEHCPKKDI"
     gene            4746..5228
                     /locus_tag="VIG33_12805"
     CDS             4746..5228
                     /locus_tag="VIG33_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEY8271763.1"
                     /translation="MLVAFFESVKYVGHLLPISFLRVFLGYYYLQQALMKFKGDFLTR
                     PRIADQIAEWLPASHAPNWYKLFMSSQIIPHWQSVAFIIVGLEFAIAISYIAGYVVRP
                     VALLGVLLCFNMLFFSGPMSEDLYKTFLAIHFIMAWVGAGRCLGFDYYFFKRRRGIWW
                     "
     gene            5236..6279
                     /locus_tag="VIG33_12810"
     CDS             5236..6279
                     /locus_tag="VIG33_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8271764.1"
                     /translation="MKHFILFITVVFVGLFIAVINKAPDENKPQESTPILRIFGYASF
                     TGQWGPGPGLKEIFEKDCGCQVQYIEGSDSGVLLQRLKIEGESLGADLVVGLDQFDLQ
                     KALAEQKWRELNISGLDVYDEVKPALQNNYFVPFDWGVLAFVIRKNELTQIPHSLDDL
                     LLPELKRKIVIEDPRTSSPGMQFLYWVIRTKGEDEGFKYIQKTMEQVHSYAPTWSAAY
                     GLFTKKQAKTALSYVTSPLYHQIEEKNNDFIALEFTEGHPLQYEFMGIPEFCKNCELA
                     EKFVNLILSDAGQKLIMQKNYMFPIIKNVKEGTPFADVPHFKTLNDFQIPSAQEVERF
                     LKKWSEIRRGEGN"
     gene            6276..7724
                     /locus_tag="VIG33_12815"
     CDS             6276..7724
                     /locus_tag="VIG33_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY8271765.1"
                     /translation="MIPRNLIRSAFLFYLIFPFLFLLAHFYILTLPDWNEFFWAFKNS
                     VLQAFFSAFFSLILGAWVALGLICFRADSRKFLELLCLFPNFLPPLFTLLSMLNFFDP
                     FPIGLTGITIAHTMINFGLVGVLLAAIIENKIGNLAELAYIEGASRGRFLRQAFWPIL
                     KHDLLLLFLFVFIVCFGSFAIPLIVGGGQGTTLEVLIYEKIRLSSNWNEAIALAIFQS
                     AFMFGVSFLAKRGLAINVERPANLHILQAISGIGVLVLLTICGLLGYFQGVVEGLHQL
                     SAFYELQSPLIWNFIGSLLIGTLVGFLSYGSLLLIAYCWPKTWFEKFLSGYLAPSTAL
                     CSFAFLIMGENESFWPFIKIPIAFSLLCLASLYRMGWDGLLHSLESQIVIARTLGAAP
                     PQIFKEVLLPQISNRAALFAGVAAVWACGDYSVSRILAHRDLSLALMTETLMSSYRLG
                     MATVLSLSLLVCGGICFSIMIGVGRVFSRKSF"
     gene            7699..8352
                     /locus_tag="VIG33_12820"
     CDS             7699..8352
                     /locus_tag="VIG33_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8271766.1"
                     /translation="MSLVENLFKNYGDFKIDIPSWEILDEGVTVLWGPSGSGKTSVFR
                     LLIGLESAVNFKWTFQDYDLAKLAVPQRRLGVVFQTLDLFPHMTAEENILFAARAREI
                     PEAQAHEKLRELAQVLQMNSFLNRPVRVLSGGEKQRVAIARALSGNPRLLLLDEPFSA
                     LDQELRHESRDLISRVIRKEKIPTLLVTHDPLDVEVLANKVSTIRQGQIVEDRLVPL"
     gene            8475..>9574
                     /locus_tag="VIG33_12825"
     CDS             8475..>9574
                     /locus_tag="VIG33_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271767.1"
                     /translation="MKLFYYNVLVEKEDLADRVEDINFLKNALKLNKKIALYAPRRYG
                     KSSISKNIIPKELKTKKFLAIYCDFMGVNTPADVAFRLSKGLAEGMEEYIPAQRLFES
                     AKQVLKNINFTFDTNPITGEASFGLSLKKEQSHSITDLLKVFLQLAKNYKCLFIFDEF
                     QDILDAKDSLSLLRSELQKLKDTPILFLGSKRRLLSQIFTSHQSPFFNFADEYVLGPI
                     PFADWSDFFDERLKPLKVKIHDEALQQIIDLSLEVPNTICEIGSYLQGKCSNESLDEN
                     KTLKLIDELVTAKSESFYFQIKQLTSAEKKILSILAVNKFTSEIQGKAFQSKTTLSLS
                     GIKKTVHKLYNQGLIEEDQNAYRVSNPLFRLF"
BASE COUNT         2467 a         1999 c         2179 g         2929 t
ORIGIN      
        1 cgggatggta ttaatctcat tcacacaaag ctcgccattt ttttgcagaa agaaatccac
       61 ccgagtcagg ccatcgcagc ccatgacttg gtaagtatgc acggccattt tttggatctt
      121 ttccacttcc gcagttgata actgagccgg aatcacgatg gaagctcctt gttcgtcagt
      181 gtacttggcg tcgtaagaat agaactcatg atgggcaatc acctcaccag gtaaagaagc
      241 ttgggccgtg gcattaagcc ccatcacgga gcattcgatt tcgcgccctt ggataaactc
      301 ctcggctaaa actttggtgt cataattgaa cgcgtctttc aaatcctttt caaaagtttc
      361 cttggctttg attttatgga ctccgacaga agatcccgca ttggcgggtt taataaagaa
      421 cggagagcct aagcttttaa ctaattcttc gtaagtcagt ctatgatggg gggtgagcaa
      481 ttcatatttt gcgttcggca ctcccgcaac tgcgagaaga cgcttcatga cttctttgtc
      541 cattcccgcc gcagaactcc aaacaccaca gcctacaaag ggaagattca tcatcttgaa
      601 aagaccttgg atggttccgt cttcgccgaa agttccatgc agaataggaa aggcgcaatc
      661 cactgcggtc ttggtttgtt tttttaaaga gaaaaaccat ggttttccgg cttctgaaat
      721 caaagccacc gggtcggaac ttgcgggtaa acttttgtca gtcaattgca cgatttgatc
      781 aaagacttcc atgccgctaa actgatacca cgaaccttct ttactaattc ccacgagaag
      841 gaagttaaac ttatttttgt ccaaggcgtt tgcgacattt ttagcggagc gaatagagac
      901 ttcatgttcc gaggatctgc caccaaaaat cagagcgatt gtttttttca tgccatctcc
      961 acaagttctt tagcgagcgc tgaaattcct ttaaaaattt catgaggttt cgcattgtca
     1021 tacatgtaag caggagtcgt gatcactttt gtttcacgat ccgtcacata gtcatcgacc
     1081 gggcaaattt catgttgcgc tccggtcttt aaaatttttg caatggtttc cttgtcgtcg
     1141 ccaactgtga cggtgacttt ttccgatcct aacacttttg cgataaggac cggcgcaata
     1201 caaatggctg cgatgggttt gctggcagca tgaaagtcga taatagcctt ttcaacttca
     1261 ggaagcaccg tgcattgggc tcctttttct gcccagttgc ttagattctt cgcggcacca
     1321 tagcctccca cgaaggccag agcatcatat ttactgacct cgagattttt taaatcttga
     1381 atttctccac gagtgattct tgcggcttca gtaagcgcat tgcgttttgt tccaagaggt
     1441 tcatttttaa taaaatccag ggcattgaat tcaatattgg gagcaaaaca cctatactcg
     1501 gcaccatttt ggctcaagga aatcaataaa ctaatggctt cggtaagttc agcaccatct
     1561 ttattgccac agccggagag tatgacggcg atttttctca tagaagacct ccgaaagaga
     1621 gaaagtttgg ctcaaactct agcgaagacg ggtgatattg tccatggaat caatgttaat
     1681 ttgttgttcc gacttcgaag ctttggactt tgttgcagat ctttacaatg actttttgag
     1741 tcgcagtggg aaccattccc aaataacaga actcaagaag acgggaaagg taagggacat
     1801 cggagctaaa agtcatttca taatttactt tctccgggtg agtcgtattg gttttttcat
     1861 ccaccaaatt tacgacaaga tcaaaagaga ctttcttata attgacttca caattgggga
     1921 ttccgccgca gtcgggtttg ctggcgacac cggtcggggc tggacgtttg ccctcagaaa
     1981 cttgcaaatt gtgacaggtc acgtcccaat ccttagcttt tacgcaggct ccgagcatat
     2041 ttcttacagt taacacgcct aaaaattgac tttgtgttcc gccggcccga aggccgacgc
     2101 tgccattggc gagagatttt tgcagggtct ccaacgaatt ggggaaggcc gttttttctt
     2161 cggccaaagc aggggtccct gtcagagtgg ggaccgtttt tggaatcgaa atatcatttt
     2221 caaaagccgt gacaggtttg aagctgccat tatccatggc ctgagagcga attaaaaatt
     2281 tataatcgac ggtgtcggca ttctctacgc gtgcgatcac ttgcgtcgct tctttataaa
     2341 ccaccttcgg atccaaggtg gcgatttttt gatcctgttc gacatagagg aattcattta
     2401 agcgaactga gttgaaatcc gcattatccc agccctcgag aatggctttt tgcacttgat
     2461 ccacggggac ttcgggacca ttgtcatcac tcagggaagg tggcttcagg cagctcgtgc
     2521 ctgcaagtag tgctaacagt aagagacttc ttttcaaggc gttgaacaga gtgttgatca
     2581 tatggagtcc cacccccaga tccagagatt caggggaaga attttagaga gtttcgtgcg
     2641 ggtttcaggt ttaaagacat ccaacatatg gaagtcaacg ggaccaaaga gcaaatgagt
     2701 cagattggtt tcgcccccaa gcactgctag atctccgttg caaccaaaaa catattggcc
     2761 atcacggctc tctaaaacga tgtcagagat tccttcagca cgaaaagcac gtttgatttt
     2821 cgctgccaac tgatcgaagt ttaaaatctt gatcattccc aggtagcctt gattataagt
     2881 caccggtttt tcgcgcaagc gttgaatcag gttttgtgag tgaggaggca cgatgatggt
     2941 aatggcttgt tttttctcac ggcggatata actcaggagc gtaatgatcg ctgtcacaga
     3001 gcctccccat tcgtgaatat atccccccag gtccgcccct tttccttcaa tggcataagc
     3061 cgctaattgg cccgaagaat cccaagcggt atagacctgt gtttgtggaa tatttaaata
     3121 cttccgggtt tcctcaatag ttcttacgga gttgacagtg tgggtggagt aaaggcgatg
     3181 aatagcttcc gctgccacct ttgtttcttt ggagaggcga aggctagggt ctggcgctgc
     3241 aaactcctct tcgatgacta aactgatttc agttccggcc agttcaaaac ccattttgcg
     3301 ataaaagtca tagagatctg tccataggat cgcaatatca cattgttgac gggtggcctg
     3361 ttgcagacaa tcttgaataa ttttagtact taaaccttgg tggcggtggg tttcgttcgt
     3421 taccacggag ccaatggagc ccactttaaa aataaggtgt ggagatttta caatcagagg
     3481 cttgagcacg gcgtgggaca caacctggtc ttcatctgta ataattctca tattatgcag
     3541 attggttgtg gaaaaagccg tggggtactc tgcagctata gaccaggtcg agtgggaccg
     3601 cagactgtga tttaagaaat ttaagactgt cggcaattct gactcagccg gtgagcgcgg
     3661 gccttccatg aatcctccta ttaggactcc tatagtttaa cgaaatcaga ttctttcttc
     3721 agtgtttgtt cgtcatttcg ggggtcttcc agccttaaat gcgacctcag tcctgaggcg
     3781 gaaattgact ttaagcggtc atgctttata ttagccctaa aattggaggc tggaatggct
     3841 gagttggatc aaaagtggaa agaaaataag catggaaaaa tgtttgttga tcagtcttgt
     3901 attgcctgtg atgcgtgcat tttaacagct ccaaagaact tcaccatgca tgaggaagat
     3961 ggccatgcgt ttgtttctaa acaacctgag actcctgaag aagaggccgc ttgtaaagag
     4021 gcgatggaag gttgccccgt ggaagcgatc ggcgactttg gggataaagc atagtctgtg
     4081 aaaaaagctc cggcgaatgc cgccattgtt ttaaaactcc tgcaaagata ttacccagat
     4141 gctcattgtg cccttaatca tcgtaatgcc tttgagcttt tgattgccac tattctttcg
     4201 gcccaatgta ctgacgagcg cgtcaatatc gttacaaaag aactctttgc gaagtatccc
     4261 agtcctgaaa aaatggccaa agctgccatt gaagacttgg aacacctcat tcgttccgcg
     4321 ggtttttata aaaataaagc taaaaatatc aaagcctgct cgcaatcctt ggtggaaaaa
     4381 tataaagggc aagttcctga aactatggag gagcttgttg gtctagcggg ggtggggcgt
     4441 aaaacggcca atgtggtttt aggcaatgcc ttcgcagttg ccagtggcat tgtggtcgat
     4501 actcatgtgg ccagattgag ctatcgtctg ggttgggttg aaagtgagaa tgcggcgatt
     4561 atcgagaaac gactttgtga gttcgttccg aaaaatcatt ggatccagct ttcccatgaa
     4621 ctcatttttc atggccgaca aatttgcaag gctcgtaagc ctcaatgtga aagatgtttt
     4681 ctttgtgagc attgcccaaa aaaggatatt tgaccccgac cgcctgtcgg gttttaatga
     4741 ggtccatgct agtagccttt tttgaaagtg taaaatacgt tggccatctt ttaccgattt
     4801 ctttcctcag ggtttttttg gggtactatt acctgcaaca ggcgctgatg aagttcaaag
     4861 gggatttttt gactcgtccg cgcatcgcgg atcaaattgc agagtggctg cctgcaagtc
     4921 acgctcctaa ttggtataaa ctttttatga gcagccaaat cattcctcat tggcaatccg
     4981 ttgcttttat catcgttggc cttgaattcg ccattgcgat ttcttatatc gcaggttatg
     5041 tggttcgtcc cgtggctctt ttaggagttt tgctttgttt caatatgtta tttttctctg
     5101 gtcccatgag cgaagatctt tataaaacct ttttggccat tcatttcata atggcgtggg
     5161 tgggagccgg tcgatgttta ggcttcgatt attatttctt caaacgtcgt cgtggtattt
     5221 ggtggtaatt tacgtatgaa acactttatt ttatttatca cagttgtttt tgtgggactt
     5281 tttattgcag tcattaataa agcccctgat gaaaacaagc ctcaagagag cacgccgatc
     5341 ttacgtattt ttggttatgc ctcttttacg ggacagtggg ggcctggacc gggactgaaa
     5401 gagatttttg aaaaagattg cggctgccaa gttcaatata ttgaaggcag tgattcggga
     5461 gttcttcttc agcgcttgaa aattgaaggc gagagtctcg gcgctgatct ggttgtaggt
     5521 ctggatcaat tcgatctgca aaaagcattg gctgagcaaa aatggcgtga acttaatata
     5581 tccggtcttg atgtttacga tgaggtgaag ccggctctgc aaaataatta cttcgtgcct
     5641 tttgattggg gagttctggc ttttgtgatt cgcaagaatg agctgactca aatcccgcat
     5701 agtttggacg atcttttact gccagaactt aaacgcaaga ttgttattga agatccgcgg
     5761 acgagctctc cggggatgca gtttttatat tgggtgattc gcaccaaagg tgaggacgaa
     5821 ggttttaagt atattcaaaa gaccatggag caagttcata gctatgcgcc gacctggtct
     5881 gcagcttatg gtctctttac caagaagcaa gcgaagaccg ctttgtctta tgtcacgtcc
     5941 cctctctatc atcagataga agagaaaaat aatgacttta tcgctttgga attcactgag
     6001 ggtcatcctc tccagtatga atttatgggt attcctgagt tctgtaaaaa ctgtgagctg
     6061 gctgaaaaat tcgtgaatct gattctttcc gacgcaggac aaaaacttat catgcagaaa
     6121 aattatatgt ttccgatcat taagaatgta aaagagggaa cgcctttcgc ggatgttcct
     6181 catttcaaga ctttgaatga tttccagatt ccctctgcac aggaggtgga aaggttctta
     6241 aagaaatggt ctgagattcg tcgtggagag gggaattgat cccacgcaat ctgattcgtt
     6301 cagcgttcct cttctatttg atttttccat ttctattttt gctggcgcac ttttatatcc
     6361 tcacattacc cgactggaat gagttcttct gggcttttaa aaattccgtt ctacaggctt
     6421 tcttttccgc tttcttttct ttaatcctcg gtgcttgggt tgctttgggg ttaatctgct
     6481 ttagggctga ttcgcgaaag ttcttagaac tactttgtct atttccgaat tttttgccac
     6541 cgctgtttac tttactgtcg atgctgaatt tttttgaccc ctttcccata ggacttaccg
     6601 gaatcaccat tgctcatacg atgattaact ttggccttgt gggggtcttg ctcgcggcta
     6661 ttatcgaaaa taaaataggc aacctcgcgg aattggctta tattgaaggg gcttctcgcg
     6721 ggagatttct gcgtcaagct ttctggccca tcttaaaaca tgatttactt ttactttttc
     6781 ttttcgtttt catcgtttgc ttcggaagtt ttgccatccc tttgattgtc gggggcggcc
     6841 agggcacaac cttggaagtt ttaatttatg aaaaaatccg tctttcaagc aactggaatg
     6901 aagccattgc cttagcgatt ttccagtccg catttatgtt tggagtttct tttctggcaa
     6961 aacgagggct tgctatcaat gtggaacgcc ctgcaaatct tcatattctc caagccattt
     7021 ccgggattgg agttttagtc ctccttacaa tatgcggact tttaggttat tttcagggag
     7081 tggttgaagg tttacaccaa ctgagtgctt tctatgagtt gcagtcgcct ttgatttgga
     7141 attttattgg cagccttcta atagggaccc ttgtgggatt cctttcttat ggaagcttat
     7201 tgctgattgc atattgttgg ccgaaaactt ggtttgaaaa atttctttcg ggttatttgg
     7261 ctcctagtac agccctctgc agtttcgctt ttttaattat gggagagaat gaatcctttt
     7321 ggccttttat aaaaattcct atcgccttca gcttactttg tcttgccagt ctctatcgaa
     7381 tgggatggga tggcctgttg cactctctgg agtcacagat agttattgct agaactttag
     7441 gagctgcgcc tcctcagatc tttaaagaag ttttattacc acagatttcc aatagagctg
     7501 ctttatttgc gggtgtggct gcggtgtggg cttgcggaga ctattcagtt tcacgaattt
     7561 tagcccatcg tgatctttcg ctggccctga tgacagagac tttgatgtca agttatcgat
     7621 tgggaatggc aacagttttg agcttgagcc ttcttgtctg cgggggtatt tgtttttcaa
     7681 taatgatcgg agtcgggcgt gtctttagtc gaaaatcttt ttaaaaatta tggagatttt
     7741 aaaatcgaca tcccctcgtg ggagattttg gacgaaggcg tgaccgtgct gtggggtccc
     7801 tctggctcgg ggaagacttc tgtgttccgg cttctcatcg gcttggagag cgccgtaaat
     7861 ttcaagtgga cctttcaaga ttatgatctg gcgaagttag cggttccaca aagacgcttg
     7921 ggcgtggttt ttcagacgct ggatttattc ccgcatatga ctgcagagga gaatattttg
     7981 tttgcggcaa gggctcgtga gattcctgag gcacaagctc atgaaaaact ccgagaactg
     8041 gctcaagtct tacaaatgaa ttctttttta aaccgaccgg ttcgagttct ctcagggggt
     8101 gaaaaacaac gggtcgccat cgctcgagcg ctcagtggca atccgcgcct gttgcttttg
     8161 gatgaaccct tctccgcttt agatcaagaa cttcggcacg agagtcggga tcttataagt
     8221 cgggtcatta gaaaagaaaa aatccctact ttactggtca cgcatgatcc gctagacgtg
     8281 gaggtcctgg cgaataaagt cagtactatc aggcagggtc aaattgtcga ggatcgcttg
     8341 gttccattat gatgtaaaaa gtcctgtgaa aatctgttta ctttttaagt ctcctctatg
     8401 acactaaaag gactacacta aaagtgtagt ccttttagtg tcatccttgt ggtctcagag
     8461 aggggaatgt ttttatgaag ttattctact ataatgttct agtcgaaaaa gaagatttag
     8521 ccgacagagt tgaagatatt aattttttaa aaaacgcact aaagctcaat aagaaaattg
     8581 cgctttatgc tccgcgaaga tatgggaaaa gttcaatttc aaaaaacatc attcccaaag
     8641 agctaaaaac gaaaaaattt ctcgcgattt actgtgattt tatgggcgtt aacacacctg
     8701 cagatgtggc ctttcgttta tccaaaggtt tagctgaagg gatggaagaa tatattccag
     8761 ctcagcgact ttttgagtca gcaaaacaag tcttgaaaaa tatcaacttt accttcgata
     8821 caaatcccat aactggtgaa gcttcttttg gtctctcttt aaagaaggaa cagagtcact
     8881 caattacaga tttactcaaa gtttttttgc aacttgcaaa aaactataag tgtcttttta
     8941 tttttgatga gtttcaggac atcctcgacg ccaaagactc tctcagctta cttcgatctg
     9001 agttgcaaaa acttaaagac actcccattt tatttcttgg cagtaaacga cgcttactaa
     9061 gtcagatctt tacaagccat caatcccctt tttttaactt tgccgatgag tatgttctcg
     9121 gacctattcc ctttgcggat tggtctgatt tttttgatga aagacttaaa cccttaaaag
     9181 ttaagattca tgatgaagct ctgcaacaga ttatcgacct ctccttagag gtgccaaata
     9241 ccatttgcga aattggatct tatttgcaag gcaagtgcag caacgaatct ttggatgaaa
     9301 ataaaacact caaactcata gatgaattag taacggctaa gtcggaaagc ttttattttc
     9361 aaataaagca gttaacatca gctgaaaaga agattttatc gattcttgct gtaaataaat
     9421 ttacttctga gatccaaggt aaagcttttc aatcaaagac aactctcagt ctttctggaa
     9481 tcaaaaagac agttcataaa ttatacaacc aggggctgat tgaggaggat cagaatgctt
     9541 acagagtatc caacccgctc ttccgattat tttt
//
LOCUS       DATBTE010000347         2594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_660665, whole genome shotgun sequence.
ACCESSION   DATBTE010000347 DATBTE010000000
VERSION     DATBTE010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660665"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VIG33_12830"
     CDS             complement(<1..202)
                     /locus_tag="VIG33_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271768.1"
                     /translation="MKNLVWIFLALLLVSVANAGSDGGVYGYAYWAPKIIHEYLQKVD
                     TYSPVEGPVKCEHFYSRGLEKGV"
     gene            complement(278..1258)
                     /locus_tag="VIG33_12835"
     CDS             complement(278..1258)
                     /locus_tag="VIG33_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HEY8271769.1"
                     /translation="MNTKGVITQFVDHHYRHFNAAALKDAAVGYKKHIDNKGQMLVTL
                     AGAMSTAELGLSLAEMIRQGKVHAISCTGANLEEDVFNLVAHNHYERIPNYRDLTPHD
                     EQKLLEKHLNRVTDTCIPEEEAIRRIENVVLEYWQEADKKGESYFPHEFMYKILLSGK
                     LEKYYQIDPKNSWLLAAAEKNLPMVVPGWEDSTLGNIFASHCIKGDVKNVHTVKGGIQ
                     YMKAWAEWYMSASKKNPVGFFQIGGGIAGDFPICVVPMLEQDLGHEDIPLWSYFAQIS
                     DSTTSYGSYSGAIPNEKITWGKLSPTTPSYIVESDATICAPLIFAYVLGW"
     gene            complement(1341..2123)
                     /locus_tag="VIG33_12840"
     CDS             complement(1341..2123)
                     /locus_tag="VIG33_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271770.1"
                     /translation="MYKIKHFKLVIICCIISLAFAACKSAKEIKTEEKTFSYQFTVNG
                     CATGQKTFSDLAAYCAALKNNAENKYCAENERLQLYSRDCNSVAPQKLSPENSEVEEN
                     QEVLRPSPQTPAQALEEPLSLKDLLIIAHPESPLRISPQPSGEFIVSALEGNLIIDSL
                     EPLFKQRLKIDQAEEITFIGADLGQCELHIKQFKAAANLESIHFTLMGTDKKNQIESS
                     GCMTKLSSIAMTGFTVEFKNVPVGGALSQQTVQKVTLKLKIK"
BASE COUNT          735 a          575 c          514 g          770 t
ORIGIN      
        1 aaacaccttt ttccaaacca cgggagtaga aatgttcgca ttttaccggg ccttctaccg
       61 gtgagtaagt atctactttt tgcaaatact catggatgat tttaggggcc caataggcat
      121 aaccataaac tccaccatca cttcccgcat tggcaacaga gacgaggaga agtgccagga
      181 agatccaaac cagatttttc attattagcc cccaatgaat cgcgaatgaa gttttttgtt
      241 cattcgcagt ccattgtcaa gcagctacta acttctctta ccaaccaaga acataagcaa
      301 agattagagg ggcgcagatc gtcgcatctg actctacaat atagcttggc gtcgttggag
      361 aaagttttcc ccacgtaatc ttttcatttg gaatcgcccc cgaataagag ccataggagg
      421 tggtcgaatc agagatttgc gcaaagtaac tccacaaagg aatatcctca tgacccaaat
      481 cttgttccag catcggaacc acgcaaatgg ggaaatcgcc ggcaatacca ccgccgattt
      541 gaaagaagcc cacaggattc ttcttagaag cggacatata ccattcagcc catgccttca
      601 tgtactggat gccccctttc acagtgtgaa cattcttaac atccccttta atgcagtgac
      661 tcgcaaaaat attgcccaaa gttgagtctt cccaacctgg aacgaccatc ggaagatttt
      721 tttccgccgc agccagcaac caagaatttt ttgggtcgat ctggtagtac ttctcaagtt
      781 tgccagaaag aaggattttg tacatgaatt catgagggaa ataagactcc ccttttttgt
      841 cggcctcttg ccagtattcc agaaccacgt tctcgatacg tcgaatggct tcttcttcag
      901 gaatgcaagt atcggtcacg cgattcaaat gcttttctaa aagtttttgt tcatcgtggg
      961 gagttaaatc acgatagttc ggaatgcgct catagtgatt atgggcaaca aggttgaaga
     1021 catcctcttc aagatttgcc ccggtgcagg aaatcgcgtg aacttttcct tggcggatca
     1081 tttcagccaa gcttaagccc aactccgcag tggacatggc ccccgctaag gtcaccagca
     1141 tttgaccttt attgtcgata tgctttttat aaccgacggc cgcatctttt aacgccgccg
     1201 cattaaagtg acgatagtga tgatccacaa attgtgttat tacgcctttg gtattcatag
     1261 cttagacctc tttttttaaa gcataaggaa taccagaaat gaaactaaag acaagtcggt
     1321 tttacgaacc ttcttattta ttatttaatt tttaacttca aagttacttt ttgaaccgtc
     1381 tgttgactga gagctccacc aacggggaca tttttaaact caacagtgaa tcccgtcatt
     1441 gcaattgagg agagctttgt catacaccca gagctttcta tttgattttt tttgtcagtg
     1501 cccatcaagg taaaatgaat agattcaaga ttagcggcag ctttaaattg ctttatatga
     1561 agctcgcact gtccaagatc agcacctatg aaagttattt cttcagcttg gtcaattttc
     1621 agtctctgct taaaaagcgg ttcgagtgag tctatgataa gatttccttc aagagcagac
     1681 actatgaatt caccagatgg ttggggtgaa attctgagcg gtgattcagg gtgggctatt
     1741 attaataggt cttttaaaga taatggctct tctaaggctt gcgcaggcgt ctggggggat
     1801 ggccttaaga cttcttgatt ttcttcgact tcggaatttt caggactcag cttctgagga
     1861 gccactgaat tgcaatctct gctataaagc tgcagtcttt cattttccgc acaatattta
     1921 ttttccgcat tgtttttaag ggccgcacaa taagcagcca ggtccgaaaa tgttttttgc
     1981 ccggttgcac agccgttgac tgtaaattga taactgaacg ttttttcctc agtctttatc
     2041 tccttagcag atttgcacgc agcaaatgca agggagataa tacagcaaat aatcaccaat
     2101 ttaaaatgtt tgattttata catatttaag ctcctcactg agctttgctg tgcaaaatac
     2161 gcgctacctc tgcagaagct gggctgtctc ttaaatgtcc aacttcttta agcataacaa
     2221 tttggggcat tttcagcccc gaattgcaca gctgttgaga tatcagagcg aaagagaact
     2281 caagaatatt ttttacgcaa ggatttattg tacacaggca aacggcataa atccagtaac
     2341 aaatgaaccc agatattttc cattttcatc ccgtgcttct atgatggccg agccgcccat
     2401 gtgggtaaac ttaccaatct gataaacttt tgcatgtcta atggatggca ggtactttga
     2461 ccacaaaatc acaagttttt atcaactctt ctgccgtggc aaaagaagat aagactgtta
     2521 aaagagaaac caaaatataa tttttcataa aacctccagt aacttaaatt gtttttatgc
     2581 cgttttttga cacc
//
LOCUS       DATBTE010000348        22950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1958972, whole genome shotgun sequence.
ACCESSION   DATBTE010000348 DATBTE010000000
VERSION     DATBTE010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 22950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22950
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1958972"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VIG33_12845"
     CDS             <1..867
                     /locus_tag="VIG33_12845"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY8271771.1"
                     /translation="ENSYHGDTFGAMSVNADGVFTKFYSDLRIDVIRCKQGQRSTDDL
                     DVWIEDVSVKIRERHHEIIGLILEPLVQGAGGMIVWPVEAVDRICTLCREYGVLLIFD
                     EVMTGLGRTGKLFAFEHLQELPDFVCLSKGLTGGLLPLALTLTSSEIYQTFLSEDVAK
                     MFFHGHSFTANAITCAAACANLKLFKKNSVLEKITVLEKIHQQSLEKIRRQVVVKDSR
                     VMGAIGAIELANDASYGGPFSQRLQALCLKNGLFVRPLGNVIYLLPPYGSEPGDLANA
                     WSIISDALKELQ"
     gene            complement(924..2255)
                     /locus_tag="VIG33_12850"
     CDS             complement(924..2255)
                     /locus_tag="VIG33_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271772.1"
                     /translation="MKKSHKIISTFFGSLLAFGIFSITFAIGEDGVLSLSDKVTRINA
                     AKMLSTGQNIFRNDTFGSEDFWGKKLRIHEALLGEKNGGVGVGISPKTALSVGLKVDV
                     EALSKTTLNGLKRAKVNLEDPATSVVLLKENAIIGLKGIFNQQGKLVSIGIQCALCHS
                     TVDNSMTKSIGRRLDGWPNRDLNVGAIINLSPDLQVVNDLLGVSDETTRKVLLAWGPG
                     KFDAELFLDGKGFRPDGKTAATLIPPAFGLAGVNLHTYTGWGSVPYWNAFVANIQMHG
                     QGNFFDPRLDNAEKYPVAARAKLGHIHSDKDLITGKLAALHYYQLSLAAPKAPKTSYD
                     PIAASRGEGVFNGKAKCASCHTPPLYTEAGWNMHDPSDIGIDDFQAKRSPDEKYRTTP
                     LKGLWTHSQGGFYHDGRFADLKAVVDHYDHHLNLGLLDNEKTDLIEFLKSL"
     gene            2596..3033
                     /locus_tag="VIG33_12855"
     CDS             2596..3033
                     /locus_tag="VIG33_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271773.1"
                     /translation="MKKYIIGMLVALSAQATFANCAASATYYNSEDDDSGVQLALALT
                     CKSSEGPVCIYLGENEAVVAGASISTADLYRQDISKVGNQLKISMKSALGLDSFLDYK
                     YVNVALNLRSLEGIVKYGGHGVSPYDGLVSHPEQSLPIECTKP"
     gene            complement(3074..3964)
                     /locus_tag="VIG33_12860"
     CDS             complement(3074..3964)
                     /locus_tag="VIG33_12860"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8271774.1"
                     /translation="MELNHLRCFYEVAKAGSFTAAARVLHISQSALSKAVALLEDREG
                     VKLFERSKKGVALTALGQDIFHQCQLVFHGVQEIEDRVRGATQKCEGLLRFGTSDHLV
                     RYLLIDRIKQFRHQYPKVTPSIFVGAPNEIIALVLKNELEFGLFFTKINTPGLEYKRI
                     TPFELVLVAETKTFPKITAKDVGDVGIVGSISREFKRHPSYRVFELTATPQKINIEAN
                     SQELQKRLCMAGEGCALLTRFMVKEELRSQKLREIKLSKPLLTDLLLVKRKGHVFTRP
                     AQKFVEEVALLLKSEDSSGK"
     gene            4113..4604
                     /locus_tag="VIG33_12865"
     CDS             4113..4604
                     /locus_tag="VIG33_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271775.1"
                     /translation="MKASLLALLITLGALVSHAEIIKSSKVQIAPSTISAVISLIPGV
                     NDKKIQVVVADHGLSTDVSPRYTIYLGFAAFAERGNITADFKITEQPVEFLSATRKSA
                     GHYTVKTREYTDGGMVEATYEIDATKVFADEHKERNRCASDFCDLDLETSISIKRTEK
                     GSR"
     gene            complement(4873..4954)
                     /locus_tag="VIG33_12870"
     tRNA            complement(4873..4954)
                     /locus_tag="VIG33_12870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4919..4921),aa:Leu,seq:gag)
     gene            5199..5378
                     /locus_tag="VIG33_12875"
     CDS             5199..5378
                     /locus_tag="VIG33_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271776.1"
                     /translation="MAKNPHRQWRLKIHGGIMSEAEMVFYFCVVYSGILSGVIWVNIL
                     ANQRVRGSFGKVKVK"
     gene            5477..6508
                     /gene="queA"
                     /locus_tag="VIG33_12880"
     CDS             5477..6508
                     /gene="queA"
                     /locus_tag="VIG33_12880"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY8271777.1"
                     /translation="MKITDLRFDYPENLVATEPQYPSRVMRMDLSSLVSGQALPQEMS
                     LQQTLELIPPGDLLVVNNTKVLKRRLFVEELEILFLSQKSEKTWEVLFPSRKFALGQK
                     ITLPGDVTMTLTRKGRPQLVELSVVVGEDYFQRWGELPLPPYIQKARGQRHTKFDDES
                     WYQTRWAQKPGSFAAPTASLHFHEAEMNFLKSRGVRVLELTLHVGLGTFLPVTSEDLD
                     QHDMHEEFAEIPAPVWDEIVQARGKGHKVWALGTTATRALESAAGGLLNRDLQGNISG
                     FTKILIQPPYQFQVVDRLLTNFHQPESTLLALVSAFAGLDTVKACYQWAIARNFRLFS
                     YGDLSIWLK"
     gene            6496..7620
                     /gene="tgt"
                     /locus_tag="VIG33_12885"
     CDS             6496..7620
                     /gene="tgt"
                     /locus_tag="VIG33_12885"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEY8271778.1"
                     /translation="MAEIGKFTLHKTAGHARRGTFTTTHGSFETPVFMAVGTKATVKA
                     MTPEELKDCGTQVVLGNTYHLHLRPNEKLIKKMGGLHKFMNWHGPILTDSGGFQVFSL
                     SQLRKMSEEGVEFRSHIDGAKHFISPEKSMEIQMDLGSDIIMAFDECLKYPATNDEIK
                     KSMDLTHRWLLRSKAAMTREESLLFGIVQGGLSLEHRKYSLDQVTSVDLPGYALGGFS
                     VGEPIHLMHEILPNIAPLMPAHKPRYLMGVGTPTDLIIAIDAGIDMFDCVIPTRVARN
                     GTLYTWQGKLSIKRTEYREDSGPLDPECDCYTCKNYSRSYLRHMFLSGEILGSRLNTI
                     HNLHFYMKLMERARHAIEIGTWAAFRDDCLTRFIKKEAAV"
     gene            7708..8046
                     /gene="yajC"
                     /locus_tag="VIG33_12890"
     CDS             7708..8046
                     /gene="yajC"
                     /locus_tag="VIG33_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY8271779.1"
                     /translation="MFWFLFGTRAFAQAAGSGAQPSGIEMFLPFIFIFVIFYFLIIRP
                     QARKQKDHQKFVTELKRGDAVITTGGILGTIEGLNENFVTLEISDGVRIKILRSQILS
                     SQVKATEGKS"
     gene            8046..9743
                     /gene="secD"
                     /locus_tag="VIG33_12895"
     CDS             8046..9743
                     /gene="secD"
                     /locus_tag="VIG33_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY8271780.1"
                     /translation="MDSLRWKSILAVLGIALGIVWVAPNFINMENKWWPSKAKLNFGL
                     DIQGGLHLVMGVDVSGVLSESTTRLANSLKAEFAKENITVTDIKTTNAEIGEMTIQTT
                     DATAKEKVREHLTKQYPTTLQEMTTGNNQIVVRYFDTYINDYKQKVIHQAIETIRNRI
                     DEFGVAEPSITQQGANRILVQLPGMADAEKAKALINTTAKLDFMIVSNEKSIQELQAM
                     ITEAEKAGGYSMATMKYSEYVNRLNNDLTGKLPEKTVLHFEKSENTTKMEIGAIPYLL
                     KTDTDLDGSALDDAFVSYGQYGEPEVALSFNSAGANQFANLTGTHVGQRMAIVLDKVV
                     KSAPSIKDRISGGHAVITLGGGRDRQKGIDEAKMISTSLRAGALPATLEQLEERRVGP
                     SLGSDSINKAKMASYIGALLIVIFMAVYYRSMGLIADICLAINIMGVFALLTSLGATL
                     TLPGIAAIALTVGFAVDANVLINERIREELRAGASWKHALSEGYHRAMSAIVDANITT
                     AATAVVLLYFGTGPVRGFAVNLLIGIVTTMFANVFLSKVIVDTLVNKFGVKKLSVGA"
     gene            9745..10716
                     /gene="secF"
                     /locus_tag="VIG33_12900"
     CDS             9745..10716
                     /gene="secF"
                     /locus_tag="VIG33_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY8271781.1"
                     /translation="MEKKTNKNTPQNTKQAMQESSGKVDFVGKVGLFGGISALLVILS
                     LIYLAVHGVSWGIDFKGGTEMQVKFTEPVTVHQVRQVAEKLNLGEVGIQGFSEQSEYV
                     IRFEGKVGKTDKETNELLNASIAKLKEAITTELGGFKPDIRRVDTVGPQVGAELKRNG
                     FLAVFYCLLVILVYVGLRFDYKYAPGAVICLAHDVIITLAIFVLVGKEVNVPVLAALL
                     SLIGFSLNDTIVVFDRIRETEHHYRDKGIHYIINKSINDMFGRTIITSGTTFVSALCL
                     YLFTDGTVSDIAFAICVGIFFGTYSSIYVAAPLVLMMEKFKVVTKPA"
     gene            10749..12464
                     /gene="recJ"
                     /locus_tag="VIG33_12905"
     CDS             10749..12464
                     /gene="recJ"
                     /locus_tag="VIG33_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEY8271782.1"
                     /translation="MTSPLWRSRDQEIAPAEGPFPPLIGQILASRGFADSQQIDKLLF
                     PKLMALADPLLLKGMGQAIERLGRAALSQEKICIYADFDLDGTSGLALLKTGMLALGF
                     TDVIHYQPKRLSEGYGFHTHAVEELYKQGVTLIVTVDVGITANPAVERAQLLGMDVIL
                     TDHHLPGAVLPPAFVIVNPNQKSDESGLGYLCGAGVAFYLLRALKRYFFDHPTLPKNN
                     FDLKEVLDFFTIATLTDMVPLLDDNRVLVKHGLLKLAETQRPGLRALLIELGLDNRAL
                     TSQDVAIRFAPKLNALSRMESGILPIDLFLVEEAAQADQMVSQVLKNNSTRVQLQSDA
                     ETEAMELLKDWTSKDFVFVASRNFHRGIVGLIATKLSQVFNKPAFVESWGEDNVIVGS
                     ARLPNGQEACLVEALSSAKDLLSRFGGHSAASGFELHSDRLSAVIERLDSYFFELHEK
                     PKALEIFYDVEARLGDVGAPLMRWYDFVGPFGVGFSIPLIYFQKVILHSKKSLKGGHW
                     RLRLVGEDNQSSHEALLFSPSPRQLQVLESEPSFLDILGELQWNYFAGQKSVQILIRD
                     LKLSN"
     gene            12477..13163
                     /gene="queC"
                     /locus_tag="VIG33_12910"
     CDS             12477..13163
                     /gene="queC"
                     /locus_tag="VIG33_12910"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEY8271783.1"
                     /translation="MRRKAVVLLSSGLDSSVNLFEAHQKEEVVLALTFGYGQKAAKKE
                     IECAAKLAATLGIPHKVLSLPWFKDFNKSSLLVDSEKVPTGAEVNILSHEQSLQTAKS
                     VWVPNRNGIFLNIGAAFAEALGATLVIPGFNAEEATTFPDNSEAFMKVLTTSLSYSTA
                     NHVQVECYTVKLQKIDIVKKGISLNMPWHLLWPCYFSQDKWCGQCESCLRSKRAFVAA
                     GVDVASLYMA"
     gene            13186..13755
                     /locus_tag="VIG33_12915"
     CDS             13186..13755
                     /locus_tag="VIG33_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF366 family protein"
                     /protein_id="HEY8271784.1"
                     /translation="MQCLFINDKFIYDGAQLKSLFAYLTYGILGNSIVSWVGPCKVSF
                     EHMVDGEDLLAKSAIEGAEMLHFIIEVFDRDLFSGVALQRLFSSIIRDYLQAEAASTL
                     GKHVLERDGDDIFWGEKKLNISIAAKSPVSTMIHLAINCTNKGTPVETCSLEDLKLSP
                     ETVALEVMGKFQKEFESILKATQKVRPLS"
     gene            complement(13781..14686)
                     /locus_tag="VIG33_12920"
     CDS             complement(13781..14686)
                     /locus_tag="VIG33_12920"
                     /inference="COORDINATES: protein motif:HMM:NF016146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEY8271785.1"
                     /translation="MKTNFVKTSFFKTMTFFSIFLSGIASCLLSSTAQAALLSDKIPY
                     YGEEFYQDLNAGVANKNLAAQIKFILKSYHTPQEGVLDQVTSNCAGPKCYVHTAIGYD
                     AARVFMFGSFYLIRDEKGYGVKDVYCDHNRMQEEFVHGNPPAPGSIPDGNVINVEHTW
                     PQSRFTGRYDTLMQKSDVHHLFPTDSQMNSIRGNGWFGEVVKDTKNLKCKNVRFGKDS
                     SGKENVFEPPVEHRGNVARALFYFSTRYDLSIEPNREAILRKWHKDDPVDEEEVDRNN
                     QIFALQRNRNPFIDYPELADKISDF"
     gene            complement(14987..15619)
                     /locus_tag="VIG33_12925"
     CDS             complement(14987..15619)
                     /locus_tag="VIG33_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271786.1"
                     /translation="MIKLNILKKELITIIFLAFGLLDSTCLANSNLCSEALKGPASDL
                     VVKDLAYESAAINRIIQLAQISKDNDHLDPELSKLIAKTLFPEGERYNNLLSKIYVFG
                     RNSKTRQELEVYKDIMLKIHKVVRLSMNFHDRGSGPSFKYFYDSYWKLFSSEKAPDGS
                     LEKEMIDYTNLLNSQTHFMITDIDGRVSERNTDHWRDFYQREDMPPWLYR"
     gene            complement(15742..16050)
                     /locus_tag="VIG33_12930"
     CDS             complement(15742..16050)
                     /locus_tag="VIG33_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271787.1"
                     /translation="MKNIFTVIGTVFLSFSAFASSGFNSADRLKAVAEATNQFLSKSV
                     VSISLSSQPSPDFESYTVKFIGNTGKCYIATMHVLIYEVGGEYKISATPAKMPLIPCP
                     "
     gene            16394..16870
                     /locus_tag="VIG33_12935"
     CDS             16394..16870
                     /locus_tag="VIG33_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271788.1"
                     /translation="MKFFIMIMSLCLANIALATNKPMVRVIVCKNMDSNSEGCNHILI
                     SRTYDGFSHISLSEYDINSKHTCSNITYLDDNEKPDLSQSPIYLSGKSSAGSRLFERA
                     FYTIAQVEIDTATGEGLMKYKSGKSPEINFKRFDEARLSECSIQKIEGFYQSKGDL"
     gene            complement(17030..17365)
                     /locus_tag="VIG33_12940"
     CDS             complement(17030..17365)
                     /locus_tag="VIG33_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEY8271789.1"
                     /translation="MLRCIGLLFLSNIFMTFAWYGHLKNFKSNALWMAILVSWGIAFF
                     EYCLQVPANRIGSDHFNLPQLKIIQEVITMLVFAGFTLLYMKQPIKLDFLWASLCMVG
                     AVYFMFRGI"
     gene            complement(17367..18980)
                     /locus_tag="VIG33_12945"
     CDS             complement(17367..18980)
                     /locus_tag="VIG33_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEY8271790.1"
                     /translation="MIDLGNSYFIVLISGVAIFLYGMSMASHSLEKLMANKVTGLMNK
                     LSGSKFLSIVVGITLTTLLQSSGAVTSMLVGFGSAGVITLRQVMGVIIGTAIGSTLTV
                     QLISLDLGQYALPLFTVAFAFYFRSKKPLFKNISMVFMGFGMLFLGLKFISISAHHFA
                     EMEMMKDFFQSLRSNPGYSLLISMVFCMFAQSSAVTIGIAMSLASVNAISLNDAMLWV
                     YGANIGTTSVALIASAGSNHIGKQVAWAHFFYKALSVLLFYPFTSIFIELLDNLDTTP
                     SRITANGHLLFNIISAVAFFPFINKGASLIEKMFPKNPEDVFGTEFVKHNNYQSSALA
                     ASYAHREIMRTADIVLGMIEDSIKLFESTDHTLIQSIKERDNKVDFLYRETKMFLLDH
                     ANKSNTVVHQNIMSMIMFLSDLERAADAIDINLITLAIKKNALHLEFSGEGLQEIRSL
                     HSQVVMVAQMAINSYQTRELCPEVIQLKRDLAKHEISLRENHIGRLNRGMRDSINTSS
                     IHLDLLSEYRHIASLLCNHAYSSQRSAEE"
     gene            19051..19503
                     /locus_tag="VIG33_12950"
     CDS             19051..19503
                     /locus_tag="VIG33_12950"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEY8271791.1"
                     /translation="MLEPGLFKRRKPLHQDKQNAVCVVAAVFYQPGEVLIFRRGPGLS
                     GAGCWEFPGGKVEPGEDFVNALHREILEEIGVHMQIEEKIGDNIHQYPEKKIHLHFYW
                     SPIPKEPFRLTDHDAFQSVKPQDLDLGLLSEADRAVVEIIKKDRRMKI"
     gene            19543..20160
                     /locus_tag="VIG33_12955"
     CDS             19543..20160
                     /locus_tag="VIG33_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY8271792.1"
                     /translation="MEVFLFPLVNVSLFPKTTKPLNIFEPRYLEMVRVAAEKKLPIAV
                     GFIEDALQVGAVKAGDRIPYVRPVAGFGYPQIVEERLNGTVLIFLQGAGKVRLGKVLE
                     RGTPYMVVEAEQVQEVVTVNPENKTKLEHLLKILTRWINIHIPDQSQQEIFMKSLAGS
                     EEIVGAFASYLVRDYDLQQMALEFNDINEKIQFLHRLAESNELTT"
     gene            complement(20166..21098)
                     /locus_tag="VIG33_12960"
     CDS             complement(20166..21098)
                     /locus_tag="VIG33_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY8271793.1"
                     /translation="MKTKSQFLYFAQFLMLYTLVVILWGAWVRISHSGDGCGASWPLC
                     NGQIIPEAHHTKTWVEFSHRLTSGFFGVLVMFLFWQGRKHFAQGDPLRKASLATLIFM
                     ITEALLGAKLVLFGLVGNNDSTLRLFMMGLHQVNSLLLTGSISLVVLFASDLPDKIKI
                     AADSPVKKPLPKWAFAFLVLFISIAVTGAFASLSTTLFPSTSLLEGLAKDFAPEAHYL
                     MRLRISHPLLATLIGGVLAIYFWLKAQEETNLLLRKCTLRAALFFSLGVVFGYITLFS
                     LAPPWMKVVHLLIAHLIWMSLLRWYILKKLKLAA"
     gene            21231..22655
                     /locus_tag="VIG33_12965"
     CDS             21231..22655
                     /locus_tag="VIG33_12965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEY8271794.1"
                     /translation="MKVVLTGATGLVGKELGKALVSQGHEIFVISRNATKARLQLPYP
                     CKIIEGDLAAGPILDERFLHPMDVVINLAGESIGGGRWNEARKRRIYESRILGTRHLI
                     KSLSVAPKIFLNASAVGYYGSHGDQDISEESAASDDFLSHVCQDWEEELLILSYGEAL
                     STTRVVALRTGMILAAKGGALEKLLPLFRRGLGGVLGDGKQWMSWIHIDDVVGLILHI
                     LDKNSIRGPVNLVSPHPVINEEFTKILASVLGVGVGPHVPAFALWTALGEMANLLLLS
                     QKVIPRVALQSDYKFLYPELKAALEQILESGEKDFFVTEQYLPWKPEEIFPFFSQARN
                     LARITPPSLGFKVLSTPPEKIQLGSEITYRMKVRGFPIKWKTVITEWTPPYRFTDTQE
                     QGPYHFWEHSHELRPFAHGTLMTDRIYYSLPMGMLGNLIGGLLARAEVEKVFEFRKET
                     LIKSLQEELGLADSALSKSEAFSI"
     gene            complement(22620..>22950)
                     /locus_tag="VIG33_12970"
     CDS             complement(22620..>22950)
                     /locus_tag="VIG33_12970"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271795.1"
                     /translation="LRAFVIGAQVTAAIERVSKNPEIEFRSNLALGGVAHTALLSKEE
                     ENLCITAVQKLGLHYAGVDFVRSHRGPLLLECNPCPGFEGIEKCTHRNLAQEVILYAE
                     SLALGKS"
BASE COUNT         6329 a         4784 c         5482 g         6355 t
ORIGIN      
        1 gaaaactcct atcatggaga cacttttggc gcaatgagcg tgaacgctga cggagtcttt
       61 acgaaattct atagcgatct acgaatcgat gttatccgct gtaaacaggg gcagcggagc
      121 acagatgatc ttgacgtctg gattgaagat gtttctgtga aaatccgtga aagacatcat
      181 gagattattg ggcttatcct tgagcctctg gttcaaggag ccggcggcat gattgtctgg
      241 cctgtggaag cggtcgatcg tatctgcacg ctttgccggg aatacggggt tctgttgata
      301 tttgatgagg tcatgacggg cctgggaagg accggcaagc ttttcgcctt tgagcatctc
      361 caggaacttc cagattttgt gtgtctctct aaaggactga cgggtgggtt gctcccttta
      421 gcattgactt taacaagcag tgaaatttat caaacttttc tgtcggagga tgttgcaaag
      481 atgttcttcc atggccacag ctttacagcc aatgcaatca cctgtgccgc ggcttgcgca
      541 aatctgaagc tctttaaaaa gaattctgta ttagaaaaaa tcacagtttt agagaagatc
      601 catcagcagt cccttgaaaa aataaggcga caggtcgtcg tgaaggactc acgagtgatg
      661 ggagctatcg gtgcgatcga gctggcgaat gacgctagct atggaggacc cttttctcaa
      721 agactccagg ccctctgtct taaaaatggc ttgttcgtgc gccctctggg caatgtcatc
      781 tatctattgc ctccttatgg ctctgaaccc ggggacctgg caaatgcttg gtccattatt
      841 tccgatgctc ttaaagaatt acagtgatta aactttgata tagaaaagac ccgtcgtggt
      901 ctctcctatc aattgctttt tgattaaagc gattttaaga actctatcaa atctgttttt
      961 tcattgtcta aaaggcctaa atttaaatga tgatcatagt gatcgacaac agcctttaga
     1021 tctgcgaagc ggccatcatg ataaaatccc ccttgagaat gggtccataa accttttaaa
     1081 ggagtcgtac ggtatttctc atctggggat cgcttggctt gaaagtcatc tataccaatg
     1141 tctgaaggat catgcatatt ccaaccggct tcagtataaa gcggcggggt gtggcaggac
     1201 gcgcactttg ctttgccgtt gaagacgcct tctccacggg aggcagcgat aggatcataa
     1261 gaggtcttag gagccttcgg agcggcaagt gaaagttgat aatagtgaag ggccgccaat
     1321 tttccagtga tcagatcttt gtccgaatgg atatggccca gttttgcgcg agcagcaact
     1381 ggatattttt cggcattgtc gaggcgggga tcgaagaagt ttccttgtcc atgcatttga
     1441 atatttgcca caaaggcatt ccaataaggc acggaccccc agcctgtata agtatgcagg
     1501 ttaactccag ccaaaccaaa agctggagga atcagtgttg ctgcggtttt accgtccgga
     1561 cgaaaacctt tgccatctaa aaaaagttcg gcatcaaatt tacccggccc ccaggccaga
     1621 agaacttttc gagtggtttc gtcagagact cccaaaagat cattcaccac ttgtaaatcg
     1681 ggtgaaagat taataattgc gccgacattg agatcccggt taggccaacc gtccaggcga
     1741 cggccaatac ttttcgtcat ggaattatcg acggtggaat ggcaaagtgc acattgaatt
     1801 ccaatggaaa caagtttccc ttgctgatta aaaattccct ttaagccaat aatggcattt
     1861 tcctttagca acaccacgct tgttgccggg tcctctaagt tcacctttgc tcttttgagg
     1921 ccatttaaag tcgtctttga taaagcctcc acatcgacct taagtccgac gctcaatgct
     1981 gtctttggac ttatgcccac gccgacgcca ccatttttct cacccaacag ggcttcatga
     2041 atacgtaatt ttttccccca aaagtcttcg ctgccgaaag tatcattgcg aaaaatattt
     2101 tgccccgttg aaagcatttt ggcagcattg attctggtta ccttgtctga gaggctcagc
     2161 accccgtcct cgccaatagc gaaagtgata ctaaaaatac cgaatgctaa aagacttccg
     2221 aaaaacgtgc ttattatctt atgagatttc ttcataggtt ctcctctttt aaaatagatt
     2281 caaatcattt ctgattttga tcctagggta aaagagaact ctttctcaaa gctaatttcc
     2341 catgcgaaga aaatgtcatg gttaggaaag taaaggagaa caaaatggca atttttgtag
     2401 ggatcttgca acggaaaatt cttactgatc gcgactagtc cactattaat attggtgtgg
     2461 gttatcctgt atcgctgtag aaattcaata ggtggaaaaa aattcaaatt tgaaatcaaa
     2521 aggcatacat ttttttgaat gtgatatatt atccaaaatt cattagtcca ccaagttaga
     2581 aagggattaa gatctatgaa aaaatatatt attggaatgt tagtcgcgct ttcagctcaa
     2641 gcgacattcg ctaattgcgc ggcttctgcg acctattata acagcgaaga cgatgattca
     2701 ggtgttcaat tggccttggc tctaacttgt aagtcttctg aaggtcctgt ctgtatctat
     2761 ttaggagaaa atgaggctgt agtggcgggc gcttcaattt caacggcgga cttgtataga
     2821 caagatattt caaaagtagg taatcagctt aagatttcga tgaaatctgc actaggtttg
     2881 gattcctttc tggactataa atacgtgaac gtggctttaa acttgagaag tcttgaaggc
     2941 attgtgaaat acggcggcca tggtgttagt ccctatgatg gtctggtctc ccatcctgaa
     3001 caaagtttac ccatagagtg cacaaaacct taatttacga aattctggat aaaacataac
     3061 ctcaagattg aatttatttt ccggaggagt cttcgctctt cagaagtaaa gcgacctcct
     3121 ccacgaactt ttgcgcaggc cgtgtgaaaa catggccttt tctcttcacc agcagcaagt
     3181 cggtaagaag aggcttcgat aacttgatct ctcgtagttt ctgcgagcgt aattcttcct
     3241 taaccataaa gcgcgtcaac agtgcgcagc cttcgccggc catacaaagc cgcttttgca
     3301 gctcttggct attagcttca atattaatct tttggggagt agctgtgagt tcaaagacgc
     3361 ggtaggaagg atgccgtttg aactcacggg aaatcgaacc tacgatacca acgtctccca
     3421 catccttggc agtgattttg ggaaatgttt tggtttccgc gactagcacc agttcgaaag
     3481 gggtgatcct tttatattct agtccaggag tgtttatctt ggtaaaaaac aagccaaact
     3541 caagttcatt tttcaacact aaggcgatga tctcattggg agcccccacg aaaatactag
     3601 gggtgacttt cgggtattgg tgacgaaact gctttattcg atcgattaat aaataccgca
     3661 ctaaatgatc ggaggttcca aagcgcaaaa ggccttcaca cttttgagtg gcccctcgca
     3721 cacgatcttc aatttcttgc accccatgaa aaaccagttg gcattgatgg aagatgtcct
     3781 gccctaaagc ggtcagggcg actccttttt tggacctttc aaagagcttg accccctcgc
     3841 gatcttctaa gagtgccacc gccttactta aagcggactg actgatatgg agaacgcggg
     3901 cggcagcggt aaaacttccc gccttagcga cctcataaaa acagcgtaag tgattcagtt
     3961 ccataaaacc attgtacgcc tatctattcc atattggaat agatagaagt caaaatagat
     4021 gcttttaaga gggatccatt tccccttaaa agaggcacat cgctgtttca gtgtgaaaca
     4081 gcataaaaaa cgcaattaaa aaggaaatta caatgaaagc cagtctctta gcacttctta
     4141 tcaccctagg tgccttagtt agccatgctg aaatcatcaa atctagcaaa gtacaaatcg
     4201 ccccgtccac gattagtgca gtgatttccc tcattcctgg agtgaatgac aaaaagatcc
     4261 aggttgtggt agcagaccac ggtttgagta ccgatgtgtc tccgcgctac acaatttatc
     4321 tcggttttgc ggccttcgca gaaaggggca atatcacagc tgactttaag attacagagc
     4381 agcctgttga gtttttaagc gcgacccgaa agtcagccgg tcactacacc gttaaaactc
     4441 gagagtatac agatggaggg atggttgaag cgacctatga aattgatgcc acgaaagtgt
     4501 ttgccgatga acataaagag agaaaccgct gcgctagcga tttctgtgat ctcgaccttg
     4561 aaacttctat ttcgataaag cgcactgaaa agggatcacg gtaattttaa gaccggtcat
     4621 tggaggaata aaaataacaa tttagaaatt ataaaatgga gcgtttgatg cggagattgc
     4681 atcgattcag gttataattc aacagcctca ttaaggacct cagcaacaaa aggatccgat
     4741 tgtctgtgat gatggggatg tactaggaga tcaatctttt gctctccaaa attcattttg
     4801 agctgaacga gaatatctat tttctgatta aaactaatga catcgctgag gatcagcaga
     4861 tcaatattta aatggtgcgc gaggggggac ttgaaccccc acgcttgcgc atacgcccct
     4921 caaacgtacg tgtctaccat tccaccactc gcgcgcaccg aaatagactt gaagaacaaa
     4981 aataagggaa cttatttaaa gcatattctg tgtgggggag tcaatcgtca aaacctgaca
     5041 agtttcggga ctcaagtcca ggttctggaa aaaatgaact caataaaata tttttgctta
     5101 aaaaaagagc gggtttgtta acccagtgag gaattctttt tgtcacaaag acagaggggc
     5161 atggatgctc cttaagacgg aaaccggggg aggctaggat ggcaaagaac cctcaccgtc
     5221 aatggagact caagattcac ggaggaatta tgagtgaagc tgaaatggtc ttttactttt
     5281 gtgtcgttta ctcggggatt ttgagtggag taatctgggt caacatactt gctaaccagc
     5341 gggtgcgggg gagtttcggt aaagtgaaag taaagtgaaa taagatttta tttgcgactt
     5401 tcttgggttt ggtggttctc caaagaaagt catgcggggc ttacgccccg catttttttg
     5461 tgttaaagat catttcatga aaattactga ccttcgtttt gactaccccg aaaatctggt
     5521 ggccactgag ccccagtacc cctcgcgagt gatgcggatg gatctgtctt ccctcgtttc
     5581 agggcaagct ctgcctcaag aaatgagtct tcaacaaaca cttgaactta ttccgccagg
     5641 tgatttgctt gttgttaata ataccaaagt cctcaaacgc cgtcttttcg tggaagaatt
     5701 agaaatttta tttctctcgc aaaagtccga aaaaacttgg gaggttttat tcccttcccg
     5761 taagtttgcc ctcggtcaaa aaatcacttt gcccggggat gtgactatga ccctgacccg
     5821 taaagggcgc cctcagttgg tggaattgag cgtggtcgtg ggagaggact atttccagcg
     5881 ttggggagag ctccctttgc ctccctatat tcaaaaagcc cgggggcaac gccataccaa
     5941 gtttgatgat gaatcttggt accaaaccag gtgggcccaa aagccgggca gttttgcggc
     6001 cccgacggcg agtcttcatt ttcatgaagc cgaaatgaat ttcttaaaaa gccggggagt
     6061 acgggtttta gaactcactc ttcatgtggg actaggaact ttcttgccgg tgacctctga
     6121 agacttagac caacacgata tgcatgagga attcgctgaa attccggccc cggtgtggga
     6181 cgaaattgtt caggcccgcg ggaaagggca taaagtttgg gcgctgggaa cgaccgcgac
     6241 tcgggccctg gagtccgcgg cgggcgggct cttgaaccgc gacttacagg ggaatatttc
     6301 tggatttact aaaatcctca ttcaacctcc ttatcagttt caggtggtag atcggctttt
     6361 gaccaatttc catcagcccg aatccaccct ccttgcatta gtttcagcct ttgcggggct
     6421 ggatacagta aaagcttgct atcaatgggc gattgcgaga aatttccgcc tctttagtta
     6481 tggggatcta agtatatggc tgaaataggt aaattcacac ttcacaaaac ggcagggcat
     6541 gctcgccgcg gaactttcac aaccacccat ggctctttcg agacgccggt gtttatggcg
     6601 gtgggaacga aggccacagt caaggccatg actcccgaag aactcaagga ctgtggcacc
     6661 caagtggtat tgggaaatac ctaccacttg catcttcgtc ccaatgagaa gctcattaaa
     6721 aaaatgggcg gcctgcataa gtttatgaat tggcacggac ctattttaac tgattccggc
     6781 ggctttcagg tgttctcttt gtcgcaactc cgtaaaatga gcgaagaggg cgtggagttc
     6841 cgctcgcata ttgatggggc gaaacacttt atcagtcctg aaaagagtat ggagatccaa
     6901 atggatctgg gctcggatat catcatggcc ttcgatgaat gtcttaagta tccagccaca
     6961 aatgacgaaa ttaagaaatc catggatttg acccatcgct ggctgctgcg ttcgaaagcg
     7021 gccatgaccc gtgaagagag tttgcttttt ggaattgtgc aaggaggctt gagtcttgag
     7081 catcgtaagt attccctgga ccaagtcacc agtgtggacc ttccgggcta tgctcttggt
     7141 ggcttcagtg tgggggagcc tattcacctg atgcatgaaa ttctaccaaa tattgcgcct
     7201 ctgatgccag cgcataagcc gcgctacctg atgggagtgg ggacgcccac ggatttaatc
     7261 atcgccattg atgccggtat cgatatgttc gattgcgtta tcccaacccg agtcgccagg
     7321 aatggaactc tttatacctg gcaaggaaag cttagtatca agcgcacgga gtatcgcgaa
     7381 gacagtggtc ctctggatcc tgaatgtgat tgctatactt gtaaaaacta ttcccgatcc
     7441 taccttcgcc atatgttttt aagtggagaa attttgggtt ctcgactgaa caccattcat
     7501 aatcttcatt tttatatgaa actcatggag cgggctcggc atgctattga aataggcaca
     7561 tgggcggctt tccgtgatga ttgcttaacg cgattcatta aaaaggaagc agcagtctag
     7621 atgaaatcct cattccaatc ataaacgatt gatttcgtta acagaacatg gtcaattttt
     7681 ctcttcaata atgtgtggag gttgttcatg ttttggtttt tgtttggaac aagagcattt
     7741 gctcaggcag ccggtagcgg agcgcaaccg tcgggcattg agatgttttt gccatttatt
     7801 tttattttcg tgatttttta ttttttgatc atccgtcctc aggcaagaaa acaaaaggac
     7861 catcagaaat ttgtcaccga gttgaagcgc ggtgacgctg tcattacaac cggaggcatt
     7921 cttgggacca ttgaaggtct caatgaaaat tttgttactt tggaaatctc cgatggagtt
     7981 cgtattaaga ttctgcgcag tcagatcttg tcgtcacaag taaaagcaac agaaggaaaa
     8041 agctaatgga cagtttgcgt tggaaaagca tattagcggt gttggggatt gccttgggga
     8101 ttgtttgggt ggctccgaat tttataaata tggagaacaa atggtggccc tcaaaagcaa
     8161 aactcaattt tgggttggat attcaagggg gcctgcatct agtgatgggc gtggatgtat
     8221 cgggtgtttt atccgaaagc accacacgtt tagccaacag tttaaaggct gagttcgcta
     8281 aagaaaacat cactgtcacg gatattaaaa ccacgaatgc tgaaattggt gagatgacca
     8341 ttcaaacaac ggatgcgacg gcgaaagaga aggtgcgtga gcatcttacc aaacagtacc
     8401 ccaccacttt gcaagaaatg acgacgggta ataaccaaat tgtggttcgg tacttcgata
     8461 cttacatcaa tgactacaaa caaaaagtaa ttcatcaagc cattgaaaca atccgcaatc
     8521 ggattgatga gttcggtgtg gcggagccaa gtattactca acaaggagcc aatcgcattc
     8581 tcgttcagct tccaggaatg gccgatgcgg aaaaggcgaa ggcattgatt aataccacgg
     8641 cgaagttgga tttcatgatc gtgtccaatg agaaatccat tcaagaactt caggcgatga
     8701 tcacggaagc tgaaaaagca ggtggctata gcatggcgac catgaaatac tcagaatatg
     8761 tgaatcgttt gaataatgat cttacaggaa agctcccaga aaagactgtc ttgcattttg
     8821 aaaaatctga gaacacgacg aaaatggaaa tcggcgcgat tccttatctt ttgaaaaccg
     8881 atacagatct ggatggctcg gctctggatg atgcatttgt cagctatgga caatatggtg
     8941 aacccgaagt ggctttgtcg ttcaatagcg ctggcgcgaa tcagttcgcc aatctcacag
     9001 gcactcatgt gggtcaacgc atggcgatcg ttttggataa agttgttaaa tcggctccaa
     9061 gcataaaaga tcgtatctct ggaggccatg cggtgatcac tttgggcgga ggccgtgatc
     9121 gtcaaaaggg tatagatgaa gcgaaaatga tttccacttc ccttcgtgct ggagctttgc
     9181 ctgcgactct agagcaattg gaagaacgcc gcgtaggtcc atctttaggc tcagattcca
     9241 ttaataaagc aaaaatggct tcttatatcg gagctctctt aatcgttatt ttcatggctg
     9301 tttattacag atccatgggt ttgattgcag acatttgttt ggcgattaat atcatgggtg
     9361 tgtttgcttt gttgacttcg ctaggagcca ctttgacttt gccagggatc gcggcgattg
     9421 ctttgaccgt gggttttgca gtggacgcga atgttctgat taatgaacgt attcgagagg
     9481 aacttcgcgc tggcgcctct tggaagcatg cactttctga gggataccat cgggcgatgt
     9541 ctgccatcgt cgatgcgaat attacaacag cggccacagc ggtggtattg ttgtatttcg
     9601 gaacaggtcc cgttcgtggt ttcgcggtga atttgttgat cggtattgtg acgacaatgt
     9661 ttgccaatgt tttcttgtct aaagtgatcg tggatacttt agtcaataaa tttggcgtta
     9721 agaaattatc ggtaggtgca taacatggaa aaaaagacaa ataaaaacac ccctcagaac
     9781 accaaacaag cgatgcaaga atcctcagga aaagtggact tcgtgggtaa ggtggggctt
     9841 tttggcggaa tctccgcgct attggtcata ctttccttaa tttatctcgc cgttcacggt
     9901 gtgagctggg gcattgattt caaggggggg acagaaatgc aggtgaagtt cactgagcct
     9961 gtgactgtcc atcaagttcg ccaggtggct gagaaattaa atcttggtga agtcggtatt
    10021 caaggtttca gtgagcaaag cgaatatgtg attcgtttcg aaggaaaagt tggtaaaact
    10081 gataaggaaa ccaacgaatt gctgaatgct tccattgcaa aacttaagga agcgatcact
    10141 acggagctgg gaggctttaa accagatatc cgccgtgtgg acaccgtggg acctcaggtg
    10201 ggtgcagagt tgaaacgcaa tggatttctt gctgtctttt attgtttgct agtgatcctg
    10261 gtttatgtcg gacttcgctt tgactacaag tatgctccag gcgccgttat ctgtttggcc
    10321 catgacgtga ttatcaccct ggccattttt gttttagtgg gcaaagaggt gaacgttcct
    10381 gtattggctg ccttgttatc gctgatcggc ttctcactga atgataccat cgtcgtgttt
    10441 gaccgtattc gtgagacgga acatcactac cgtgataaag gtattcacta catcattaat
    10501 aagtcgatca atgatatgtt cggtcgtacc atcattactt ccggaactac tttcgtttca
    10561 gcgctgtgtt tgtatttatt tacggatggc actgtttccg atatcgcctt cgcaatttgc
    10621 gttggaattt tcttcggtac ttactcctct atctacgtag cggcaccttt ggtgttaatg
    10681 atggagaaat ttaaagtggt cacaaaacca gcttaattgc tggttctctc aaattgttag
    10741 gagtctggat gacgagtccg ctatggagat cgagggatca agaaattgca ccggcagagg
    10801 gtccgtttcc acccttaatc ggtcaaattc ttgcctctcg gggctttgcg gactcccagc
    10861 agattgataa acttcttttt cctaaactta tggcactcgc ggatcccttg cttttaaaag
    10921 gcatgggtca ggcgattgaa cgtctggggc gtgcggccct tagccaagaa aagatctgca
    10981 tttatgccga ctttgattta gatggaactt ccggtttggc cttattaaaa accgggatgc
    11041 tcgccttagg atttacggat gtcattcatt atcagcccaa gcgcctctca gaaggctatg
    11101 gttttcatac tcatgcggtc gaagagctgt acaaacaagg tgtgaccttg attgtaacag
    11161 tggacgtggg gattacggcc aatccagcgg tggagagagc tcaactgctc ggaatggatg
    11221 ttattcttac agaccatcat cttcccggag ctgttttgcc tccggctttt gtgatcgtga
    11281 atccgaatca aaaatctgat gagtcgggct taggttatct ctgtggagcg ggagtggctt
    11341 tttatcttct ccgcgccttg aaacgatatt ttttcgatca tcccactttg ccaaaaaata
    11401 atttcgatct gaaagaagtt ttagattttt ttacgattgc gactttaacc gatatggttc
    11461 ctttgcttga tgacaatcga gtccttgtta aacacggttt gttaaagctg gccgaaaccc
    11521 agcgtccggg cttgagagct ttattgatcg aactgggttt ggataatcgg gctctaacca
    11581 gtcaagatgt ggcgattcgc tttgcgccta agttaaacgc tctcagccga atggaatcag
    11641 gcattctccc cattgattta tttttagtgg aagaggctgc ccaggcggat caaatggtca
    11701 gtcaagttct taaaaataat tccacgcgag tgcaactgca aagtgatgcc gagacggaag
    11761 ccatggagct tttaaaggat tggacttcca aggattttgt atttgtcgct tcccgaaact
    11821 ttcatcgcgg tattgtgggg ctgattgcaa cgaagttgtc ccaggttttc aataagccag
    11881 ccttcgtgga gtcttggggt gaagacaatg tgattgtcgg aagtgcgcgt ctgcccaacg
    11941 gtcaagaggc ctgtctagta gaagcattga gctcggcgaa ggatcttctc agccgctttg
    12001 gggggcattc ggcggcttca ggatttgaat tgcacagtga caggttgtcg gcggtgattg
    12061 aacgcttgga ttcttatttt tttgaactgc atgaaaagcc caaagcgctg gaaatcttct
    12121 atgatgtgga agcaagactt ggggatgtgg gggctccttt gatgaggtgg tatgatttcg
    12181 tcggaccttt tggagtgggt ttctccattc ccttgattta ttttcaaaaa gtgattttgc
    12241 actctaagaa aagtctaaaa ggtggtcatt ggcgtcttcg cttggtggga gaggataatc
    12301 aatccagcca tgaggctctt ttgttttcac cttcgccgcg acaactccag gttcttgaaa
    12361 gcgaaccttc attcttggat atcctcggag aacttcagtg gaattatttt gccggccaga
    12421 agagtgtgca gattttaatt cgcgatttaa aacttagcaa ttaaggttta aaacatatga
    12481 gacggaaagc cgttgtttta ctatcttcag ggttggattc ttcagtgaat ctctttgagg
    12541 ctcatcaaaa agaagaagtt gttttagcgt tgaccttcgg ctacggtcaa aaagccgcaa
    12601 aaaaagaaat cgagtgtgcg gcaaagctcg cggccactct ggggattccc cacaaggtgc
    12661 tctcgctgcc ctggtttaag gattttaata agtcgtctct cttagtggat tcagagaaag
    12721 ttccgactgg ggctgaggtc aatattttga gccatgaaca gtctttgcaa accgcaaagt
    12781 cggtgtgggt gccgaaccgc aatggaatat ttcttaatat cggagcggcc tttgccgagg
    12841 ctttaggtgc cactcttgtc attcctggtt ttaatgcgga agaggccacg acctttccgg
    12901 ataattcaga agcgtttatg aaggtcctga ccacttcact gtcttattcc acggcaaatc
    12961 atgttcaagt ggagtgttac actgtaaagc tgcaaaaaat cgatattgtt aaaaaaggga
    13021 tatctttgaa tatgccttgg catcttctct ggccatgtta tttttctcaa gacaagtggt
    13081 gtggacagtg tgaatcttgc ctgcgttcaa agcgcgcctt tgtcgccgca ggtgtggatg
    13141 ttgccagttt gtatatggct tgagcaagcc agctgaggag aatgtatgca gtgtcttttt
    13201 attaatgata aattcatcta tgatggggct cagttaaagt cattgtttgc ctatctgacc
    13261 tacggaattc tcggtaactc catcgtcagc tgggtaggcc catgcaaagt gtcttttgaa
    13321 cacatggttg atggtgagga tctcttagcc aagtccgcta ttgaaggggc tgaaatgctg
    13381 cattttatta tcgaagtttt cgatcgggat ctgttttcgg gggtcgcctt gcaaagactt
    13441 ttctcatcaa tcatccgcga ttatttacag gcagaagcgg catcaacttt aggcaaacac
    13501 gttttggaac gtgatgggga cgatattttc tggggtgaaa aaaagctgaa catcagcatc
    13561 gctgcaaagt ctccggtttc tacgatgatt catttagcaa ttaattgcac caataaggga
    13621 actcctgttg aaacctgttc attagaagat ttgaaacttt cgccagagac ggtggcactc
    13681 gaagtgatgg gtaagtttca aaaagaattt gaatcgatct taaaggcgac tcaaaaagtc
    13741 aggcctttaa gctaaagtct gaattgctac tagagcgaat ttaaaaatca gagattttgt
    13801 ccgctaattc agggtaatca atgaacggat tacgattgcg ttgaagagcg aagatctggt
    13861 tgttgcgatc gacttcttct tcatcaacag ggtcgtcttt gtgccacttg cgaagaatcg
    13921 cctctcggtt gggttcaata gacaaatcgt agcgcgtaga aaaatagaat aaggctcgag
    13981 ccacgtttcc gcgatgctcc actggcggtt caaagacatt ctctttccct gaagaatctt
    14041 ttccaaaacg cacgttttta cattttaaat tcttagtgtc cttaacgact tccccaaacc
    14101 aaccatttcc ccggatagaa ttcatttggg aatccgtcgg aaataaatgg tgaacgtctg
    14161 atttctgcat caacgtatcg tatcggcctg taaagcggct ttgtggccag gtgtgctcca
    14221 cgttaatcac attgccatca ggaatactcc cgggcgccgg cggattgcca tggacgaact
    14281 cttcttgcat gcgattgtga tcacagtaga catcttttac gccataaccc ttctcatctc
    14341 tgatcagata aaagctgccg aacatgaaca cgcgggcggc atcatatcca atcgcggtgt
    14401 gaacatagca cttaggccct gcacaatttg aagtcacttg atcaaggact ccctcttgcg
    14461 gagtgtggta agatttcaag atgaacttga tttgtgcggc gaggttttta ttcgccacac
    14521 ctgcattgag atcttgatag aactcttcgc cataataagg aattttatca ctcaaaagag
    14581 ccgcttgagc ggtgctggaa agtaggcagg aagcaatgcc ggaaagaaag atcgagaaaa
    14641 aagtcattgt cttaaagaaa ctcgttttaa caaagtttgt cttcattatt ctggcccctc
    14701 ttgtttttct gtgtagaagt tgtatcgcag aatcatagag tggaagtcaa tgaatactaa
    14761 agtatgctga aggcgcggcg taaaagaatt taaagcattt tttttcggag gcaggatttt
    14821 atccagcaaa ctcatagacg ggcggtcccg ggcccaagcg gcccagaggc cgcggtatta
    14881 ctgattttta tgtagagatt tatattacaa cgcttcggtg ccgccgctct ggctaaaaca
    14941 aagtaatggt cttacattga gggagtcgca gccagattaa atggacttat cgatacaacc
    15001 aaggaggcat atcctcccgt tgataaaagt ccctccaatg gtctgtattt ctttctgaaa
    15061 cccgcccatc tatatctgtg atcataaaat gagtctggct attaaggaga ttggtgtagt
    15121 caatcatttc cttctctaag ctgccatctg gggctttttc agaactaaat aatttccagt
    15181 agctatcata aaaatactta aaactggggc cacttccacg atcgtgaaaa ttcatggata
    15241 aacgcacaac tttatggatt tttagcataa tgtctttata tacttctaat tcttgtcttg
    15301 ttttcgaatt ccttccaaaa acataaattt tagagagtaa attattataa cgttctcctt
    15361 ctggaaagag ggttttagct attaatttag acaactccgg atccagatga tcattatctt
    15421 tgcttatctg agcgagttgg atgatacgat taatagcggc cgattcatag gcaaggtcct
    15481 tcaccactaa atcagatgca gggcctttaa gggcttcact acaaaggttt gaatttgcaa
    15541 gacaggtgga gtcgagtaaa ccgaaggcca gaaagataat tgtaatcaat tcttttttta
    15601 atatatttaa cttgatcata ctattgatac ctttcctagt ttctgtgaga ttcatttctc
    15661 aatttacagg ggtaggttcc tttgatgagt ttatgaagca atacgtgtgc caaatttgtg
    15721 gattgataaa atcgcctcca attaaggaca aggaattagt ggcattttag caggagtggc
    15781 actaatttta tattctccac caacctcgta gatcaacaca tgcattgtag ctatatagca
    15841 tttaccggtg ttaccaataa atttaactgt gtacgactca aaatctggtg atggttgaga
    15901 ggataaagag attgaaacta ccgatttgct caagaattga tttgtagcct ctgcaactgc
    15961 cttgagtcta tctgcagaat tgaatcctga gcttgcaaaa gctgaaaatg aaagaaaaac
    16021 ggtgcctata acagtaaaaa tatttttcat gtttacttcc ttcgtgtagc tgataatagc
    16081 gagagttgtg ccagcctctt tcttgattta attgtttttg agctagaata aagatgtatc
    16141 agtactttac aaagtgccgt catttgttcc ctggggtgac tctccgagtt ttcaaattga
    16201 acaaaagttc gacgaatttt caggtcactt tgtaaagtgc ttagacacgt ttttgccttc
    16261 agatcaaaga aaacaaatct ttaggttggc atcattgtcg cattatgagc tatctgtaac
    16321 tgttgtattc taaaaagctg ggaaatcaat cccacagact ttgcaacggt atcaaccaat
    16381 aagggagcga aatatgaaat tttttattat gatcatgtca ttgtgtttgg caaatatagc
    16441 ccttgcgacg aataaaccca tggtaagggt cattgtttgt aaaaacatgg atagcaatag
    16501 tgagggatgt aatcacattc tcattagcag aacatacgat ggattttctc atatatccct
    16561 ctcagagtac gatattaatt caaaacacac atgctcgaac ataacttatc tcgatgacaa
    16621 tgaaaaacct gatctcagcc aaagtcctat atatctctct ggaaaatctt ccgctggcag
    16681 tcgcttattt gaaagagctt tttataccat agcgcaagtc gagatagata cggctacagg
    16741 tgagggatta atgaaatata agagtggaaa aagtccagaa atcaacttta aacgttttga
    16801 tgaagcacgg ctttctgagt gctcaatcca aaaaattgaa ggcttttatc aaagcaaagg
    16861 agatctgtaa ttttcacctt cggttaaaga accacttagg caatttcgaa tcttggcagt
    16921 cgaggacgtg agttatgctt catgcggctc ggcccgggac tttggggaaa gccaatgcgg
    16981 ctgccttttg attccgaaag aatgagatcg aatgcagcat aagaacgaac taaatcccgc
    17041 gaaacataaa gtaaaccgcg ccgaccatgc agagactcgc ccataaaaag tccaacttga
    17101 tcggttgctt catgtaaagc agggtgaagc cggcaaagac gagcatggtg atgacttctt
    17161 gaatgatctt aagttgcggc agattgaaat gatctgagcc gatgcgattg gccggaacct
    17221 gcaagcagta ttcaaaaaaa gcgattcccc aactcacgag gatcgccatc cagagagcat
    17281 tggatttaaa attctttaaa tgtccatacc aagcaaaagt catgaagata ttcgaaagaa
    17341 acaaaagccc tatgcaacgt aacattttat tcctccgcgg atctctggct tgaataagcg
    17401 tgattgcaaa gcaggctggc gatgtggcgg tactcgctca gtaaatccaa gtgaatggag
    17461 ctggtattga tggagtctct catgcctcga ttcaagcgtc caatgtggtt ttcccgcagg
    17521 gagatttcat gtttggcgag gtctcttttt aattggatga cttcagggca gagttctcgg
    17581 gtttggtagg aattaatggc catttgagcc accatgacga cttggctgtg cagactgcga
    17641 atttcctgaa gaccctcgcc ggagaattcc aaatgcaaag cattcttttt gatggccaaa
    17701 gtaataaggt tgatatcaat ggcatccgcc gcacgttcaa gatcacttaa gaacatgatc
    17761 atgctcataa tattttgatg gaccacggta ttgcttttat tggcgtgatc caagagaaac
    17821 attttggtct cacgataaag aaaatccacc ttgttgtcgc gttccttgat ggattggatc
    17881 aatgtatggt ctgtggactc gaaaagcttg atagagtctt caatcatgcc gagaacgata
    17941 tccgccgttc gcataatctc gcggtgagca taggaagccg ccagggcgga actttgatag
    18001 ttattgtgct ttacgaactc cgtcccaaag acgtcttcag gattttttgg aaacattttt
    18061 tcaatgagac tggcgccttt attgatgaaa ggaaaaaatg ccacggcgga aataatatta
    18121 aaaagtaaat gtccatttgc ggtgatgcga gaaggagtgg tgtctaaatt atctaggagt
    18181 tcgataaaga tactggtaaa aggataaaag agaagaacac ttagcgcctt atagaagaaa
    18241 tgagcccagg ccacttgctt gccgatatga ttgctaccgg cggaggcaat gagcgccacc
    18301 gaagtcgttc caatgttcgc accataaacc cacagcatgg catcgttcaa gctgatggca
    18361 ttcacgctgg cgagactcat ggcgatacca atggtcacag cgctgctttg cgcgaacatg
    18421 cagaaaacca tggagatcaa taaagagtaa cctgggttgc tacgcagaga ttggaagaag
    18481 tccttcatca tctccatttc tgcgaaatga tgagcgctga tgctgatgaa tttcaacccc
    18541 agaaagagca tgccaaatcc cataaaaacc atggagatgt ttttaaagag aggttttttg
    18601 gaacgaaaat aaaaagcaaa ggccacggtg aaaaggggca aggcatattg cccaagatcg
    18661 agggagatta actgaacggt cagggttgag ccaatcgcag taccgatgat aacgcccatc
    18721 acttggcgaa gggtgatgac gccggcagag ccaaagccca caagcatgga ggtcacagct
    18781 cccgaacttt gcaggagagt ggttaaagta ataccgacga cgatcgaaag aaacttactc
    18841 ccggagagct tattcataag gccagtgact ttgttcgcca tgagtttctc aagggagtgg
    18901 cttgccatgc tcatgccata caaaaaaatc gcgacgccac tgatgagaac gataaaatac
    18961 gaattgccaa gatctatcat gagattaaat atagcaaaaa aaatttgtat ttgggaatga
    19021 tcttctttgt ttcaatcgaa atggcgagct atgcttgaac ctggactttt taaaaggaga
    19081 aaacctctgc atcaagataa gcaaaatgcg gtttgcgtgg ttgctgccgt tttttaccaa
    19141 cccggcgaag tgttgatttt tcgtcgaggt ccaggtttaa gtggcgcggg ttgctgggag
    19201 tttcccggcg gaaaagtgga gcccggtgaa gacttcgtaa acgctcttca cagggaaatt
    19261 ctcgaggaga taggtgttca tatgcagatc gaagaaaaaa ttggtgacaa tattcaccaa
    19321 tacccagaga aaaaaatcca tctgcatttc tattggtcgc cgatcccgaa ggagcctttc
    19381 cggctgacgg atcatgatgc ttttcaatcg gtaaagcctc aggatttgga tctcggtctt
    19441 ctttcagagg cggatcgcgc cgttgttgaa atcataaaaa aagatcgtcg catgaagatc
    19501 tagctggctt ttgagtctgg tcctttctat actgaatcca tcatggaagt ttttttattc
    19561 cctttagtaa acgtaagtct ttttccaaag accacaaagc ctttgaatat ttttgagcct
    19621 cggtatttgg aaatggtcag ggttgccgct gaaaaaaagc ttcccattgc tgttggcttt
    19681 attgaggatg ccctgcaagt gggggctgtc aaagcggggg ataggatccc ctatgtccgc
    19741 cccgtggccg gatttggcta tccccaaatt gtcgaagaaa gactcaatgg aacggttctg
    19801 atctttttgc aaggagccgg aaaggttcgc ttaggaaaag tcttagaacg gggaacccct
    19861 tatatggtcg ttgaggcgga gcaagtccaa gaggtggtga cggtaaatcc agaaaacaag
    19921 accaagctcg aacatctttt aaaaatcctc actcgctgga ttaatatcca cattccagat
    19981 caatcacagc aagaaatttt catgaagagt ctcgcaggtt ccgaagaaat cgtgggggca
    20041 ttcgcctcct atctcgtcag agattatgac ctgcaacaaa tggctctaga atttaatgat
    20101 attaacgaaa aaattcagtt tctgcatcgg ctggcggaat ccaacgaact caccacttaa
    20161 ttttcttagg ctgccagttt taattttttc aagatatacc agcgaagcaa gctcatccag
    20221 atgagatgag cgatcagcaa gtggaccact ttcatccatg gcggggctaa gctaaataaa
    20281 gtgatatagc caaacaccac acccaaagaa aagaatagag ctgctcgaag agtgcattta
    20341 cgtaaaagca ggttcgtttc ttcttgagct ttcaaccaga aatatattgc cagcacgcct
    20401 ccaatgagag tggctaacaa gggatgagat atgcgtaggc gcattaaata atgagcctcg
    20461 ggagcaaagt cttttgctaa gccctctaac aacgaagtcg aaggaaaaag cgtggttgag
    20521 agtgaggcaa aagctccagt gacggcgatg ctgataaaca atacgagaaa agcaaaggcc
    20581 cattttggca gcggcttttt tacgggggaa tcagcggcga tttttatttt atctggaaga
    20641 tcactggcga aaaggaccac caaagaaatg cttccagtaa gcaacagaga gttcacttga
    20701 tgcaatccca tcataaataa acgcaaggtg gagtcgttgt tgccgacgag gccgaaaagc
    20761 actagcttgg cgcctaacag tgcttcagtg atcataaaga tcaatgtggc taacgaagct
    20821 ttacggagag ggtcgccttg ggcgaagtgt tttcttcctt gccaaaaaag aaacatcaca
    20881 agaactccaa aaaaacctga agtgagacga tgactaaact ccacccaagt ctttgtgtgg
    20941 tgggcttcag ggatgatttg gccattgcaa aggggccaac tggcgccaca accatcccct
    21001 gaatgggaga ttcgcaccca tgctccccac aaaatcacga ccaaagtgta gagcatgaga
    21061 aattgagcaa aataaagaaa ttgagatttg gttttcatag ggcctggcct atcacagccc
    21121 cctagagggg tcaatggcaa gcggcaaggc tttgtcgaga accagctcta gcgagactct
    21181 tgaattgaga atgcttatta agtaaagtta aaaactatat agagggtatt atgaaagtag
    21241 tgttgacggg agcgacaggg cttgttggta aagaactggg caaagccctt gtgagccaag
    21301 gacatgaaat tttcgtgatc agccgcaatg ctacaaaggc ccgcttacaa ttgccatacc
    21361 cgtgtaagat tattgaaggg gatttagccg cagggcctat tcttgacgag cgctttttac
    21421 atcccatgga tgtggtgatt aatcttgctg gggaatccat tggtggtggt cgatggaatg
    21481 aagctcgcaa gcgtcgcatt tatgagtcgc gtattttagg gacccgtcat cttatcaaga
    21541 gtttatccgt agctcccaaa atatttttaa atgcctcggc cgtgggctac tacggttcgc
    21601 acggagatca agatatttct gaagaaagtg ccgcaagtga tgattttctt tcacatgtat
    21661 gtcaggactg ggaggaagag cttttgattt tatcttatgg tgaagccctt tccaccaccc
    21721 gcgtggtggc gctgcggacg gggatgattc tcgcagccaa agggggagct ctggaaaagt
    21781 tattaccctt gtttcggcga ggtctcggag gagtcctggg cgatggcaaa caatggatga
    21841 gctggattca tattgacgat gtggtggggc tgatcttgca cattcttgat aagaattcga
    21901 ttcgcggccc ggtcaatttg gtttcacccc atcctgtgat caatgaggaa ttcactaaaa
    21961 tcttggcctc agttttaggg gtgggagtgg ggccgcacgt tcctgccttt gcgctttgga
    22021 ccgctttggg cgagatggcg aatcttttgc tgctctctca aaaagtcatt ccccgggtgg
    22081 ccctgcaaag tgattataaa ttcttatatc ctgaacttaa agcggcactc gagcaaattc
    22141 ttgagtcagg agaaaaagat ttctttgtga cggaacagta tttaccttgg aagccagaag
    22201 aaatatttcc ttttttcagc caagcaagaa atttagcaag aatcacacct ccgagtttgg
    22261 gattcaaggt tctctccact ccgcctgaaa agatacagtt gggaagtgaa atcacttatc
    22321 gtatgaaggt caggggcttt ccgataaaat ggaaaacagt gatcacggaa tggacccctc
    22381 cgtatcgttt cactgatacg caagagcaag ggccttacca tttctgggag cacagccatg
    22441 aacttcgtcc ctttgcacat ggaacattaa tgacggatcg aatttattat agcttgccaa
    22501 tggggatgct ggggaatttg atcggcggcc tccttgctcg agcggaagtg gaaaaggttt
    22561 ttgagttcag aaaagagact ttaattaaaa gtttacagga agagttaggc cttgcagatt
    22621 cagctctttc caagagcgag gctttcagca tataaaatca cttcttgtgc aagattcctg
    22681 tgagtgcatt tttctatacc ttcaaagccg gggcaggggt tgcactccaa taggagaggt
    22741 cctctgtgag atctgacaaa atccactccc gcatagtgaa gccccaattt ttggaccgcc
    22801 gtaatgcaaa ggttttcttc ttctttgctt agtaaagcgg tatgagccac accgcccagg
    22861 gccagattcg agcgaaattc tatctccgga tttttagaaa ctctttctat ggcggccgtg
    22921 acttgagctc caatcacaaa ggcgcggaga
//
LOCUS       DATBTE010000349        18166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_183281, whole genome shotgun sequence.
ACCESSION   DATBTE010000349 DATBTE010000000
VERSION     DATBTE010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 18166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18166
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183281"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VIG33_12975"
     CDS             complement(<1..730)
                     /locus_tag="VIG33_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY8271796.1"
                     /translation="MRFIKSPAVTGLFILNLLLVVNGCTSNYQKLSTKPVIKVNDHVL
                     TAKEFSNQLARKLKDLDALTAKDPSTVHRTKEQLLRSFLVKSLTLDWARANSLVINEN
                     DIDKEVDKYRANYPDDLSFRRLLAQENISFSEWRENLRYTLIERAVFKKLNERIKPLS
                     EIEIKHYYDEHKEQFRKKERIYVRQIVTDEQGKADLLKSQVKSKDFAELAKTYSVAPE
                     AKAGGLVGWVEIGNIDFFDPLFKAP"
     gene            complement(744..1565)
                     /locus_tag="VIG33_12980"
     CDS             complement(744..1565)
                     /locus_tag="VIG33_12980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY8271797.1"
                     /translation="MTAIINAFSVLLLSTSLSYAQSKASEVVATVGNKRITLDDFNKK
                     FNEIKSQTLNPPTKIQFLEDMVRYEVGLQEAAKRNLEKDPIVIERIQQETYKALLEKD
                     IGQKVQKIQVSEPEMKAWYKNNPEIRTSHILIEFKAGATREQIAEAQKRANEIYEEVR
                     KSKRPFEELVKLYSDDPLSKQAGGDVGWQSRVTLVPSYYEAALSGKVNEIKGLVETPF
                     GFHVIKITGRRSYEDANKRQVRAAVFDEKRKQAFNEYFEKLKKGYAIKTNPSLLK"
     gene            complement(1630..2208)
                     /locus_tag="VIG33_12985"
     CDS             complement(1630..2208)
                     /locus_tag="VIG33_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HEY8271798.1"
                     /translation="MQLIEPECLQKNIKDKNPFLLAHWHGDEIALIQLVRRYRIATMS
                     SQSKDGQMMSIILRLLGAKTTQGSSSRGGVGGLKGLIRLMREGHNCSFAVDGPKGPIY
                     KVKPGIFELSRLLNCPIYYCGVSVSSAIHFPNAWNKTFLPKPFSKIIIQWHGPLPAMT
                     KDQDPRDPKFSTQLENLLHRAKQQALKVIAES"
     gene            complement(2280..3248)
                     /locus_tag="VIG33_12990"
     CDS             complement(2280..3248)
                     /locus_tag="VIG33_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY8271799.1"
                     /translation="MSKVFVFAEHKNGTLKRGAMELLQAAQKSGNTVIGMVVGTGAEK
                     AATEMGYHGAQEVHVIKDSNLDAYNPELYLSALSSVLEKTQPQILLASATSTAKDLFP
                     RIAAHLKTGVVSDCVELSISGDSITAKKPMYSGKAFAIANFENSPLKIVLMRANQLPV
                     AAADTSKTMVLVEHALTKPDLKTVIKEIVKGASEKLDLTEANIIISGGRGLKEAGNFK
                     LLNELADVLGATVGASRAVVDAGWVSHGMQVGQTGKTVAPTLYIAVGVSGAIQHLAGM
                     SGSKVIVAINNDPGAPIFQKATYGIVGDLFDVIPKLTEEFKKVLKH"
     gene            complement(3257..4039)
                     /locus_tag="VIG33_12995"
     CDS             complement(3257..4039)
                     /locus_tag="VIG33_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY8271800.1"
                     /translation="MKIFVCIKQVPDTETKIKINPDATSIDTTGIKWVINPYDEYAVE
                     EAIKYREANASAGAQVFVLSVGPSKRVSEALRTALAMGADEGIVINAPEDIDCYSTAR
                     ALAEVIKSEKDTGLIFTGKLAIDDNASSVSQMLGEFLGIPHATVVSKFAWSPEGVSVE
                     RDIEGGAKEIINMTAPAIIGANKGLNTPRYASLPGIMKAKKKVLKEIEFASLNIPATD
                     IKVKYSQLSLPAEKPSVKMLMGDSAHQVAELVQLLRDEAKVL"
     gene            4207..4887
                     /locus_tag="VIG33_13000"
     CDS             4207..4887
                     /locus_tag="VIG33_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEY8271801.1"
                     /translation="MALIASHGFLRSTIGKKYLMGLAGLVWTGFVLVHMAGNLLIFVS
                     PDAYNAYGHAITSGNIVYIAEAVLILALIVHVGLAFRLTMENRAARSSTYAVKAKGEK
                     SATLASQTMGIQGSLILAFIILHIATFKYGTHYDTTVKGVVIRDLYRLIAEVFQQPGY
                     VVWYLVSLVVLGFHLSHGVGSVFQSFGWMNGRHQPLLKKISYLYAGIVALGFISQPIY
                     IFLIAGKG"
     gene            4900..6816
                     /locus_tag="VIG33_13005"
     CDS             4900..6816
                     /locus_tag="VIG33_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEY8271802.1"
                     /translation="MSKILDGKVPDGAINKKWTDHKFHMKLVNPANKRKHSVIVVGTG
                     LAGASAAAAMGELGYKVKAFCVHESPRRAHSVAAQGGINAAKNYQNDGDSIYRLFYDT
                     VKGGDFRAREANVHRLAEVSVNIIDQAVASGVPLAREYGGLLDNRSFGGAQVSRTFYA
                     RGQTGQQLLLGAYSAMMRQVDLGTVELNTRREMLDLVVVEGHARGIVVRNLHTGEIES
                     HEADAVIIASGGYSNVFYLSTNAMACAVTAAWKAHKRGAYFANPCYTQIHPTCIPVHG
                     ENQSKLTLMSESLRNDGRVWVPKAKGDKRHPNEIPDDERDYYLERVYPSFGNLAPRDV
                     ASRQAKYRCDEGHGVGDSGKAVYLDFRDAIVRLGEAKVSERYGNLFQMYEKITGENPY
                     KQPMRIYPAPHYTMGGLWVDYNLESTIPGLFVAGEANFSDHGANRLGASALMQGLGDG
                     YFVLPYTISNYIASTKLNKVSTAHAAFKEAETSVKNEITKIMSIKGTRTVESFHKELG
                     HIMWEYCGMARNEAGLKKALELIPKLREEFWKNVRVPGEVNYLNSELEKAGRVADFLE
                     LGELMCRDALERRESCGGHFREEFQEDGEAKRDDQDFSHVAAWEWNGPNQPQTRHKEE
                     LKFENVKLATRSYK"
     gene            6826..7620
                     /locus_tag="VIG33_13010"
     CDS             6826..7620
                     /locus_tag="VIG33_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEY8271803.1"
                     /translation="MSELTKKINLTLRVWRQKSPKDQGGFREYAAKNVSTDASFLEML
                     DAVNEELINKGEDPIAFDHDCREGICGSCGFMIDGQAHGPQKSTTVCQLHMRAFNDGE
                     VLTLEPWRATAFPIVKDLLTDRTALDRIITSGGYISANTGSARDANSILIPKHDADHA
                     FGAAACIGCGACVASCKNASAALFTSAKISHLALLPQGQVERKIRAERMVAQMDAEGF
                     GACSSTGSCSAACPKDISLVNIARMNREFFKATILKEDEADTGGGF"
     gene            7770..8030
                     /locus_tag="VIG33_13015"
     CDS             7770..8030
                     /locus_tag="VIG33_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271804.1"
                     /translation="MKKLSELMVELGFDPEGSSAVKEAFIKYLIKASEGMSVLPPTEN
                     AIIKANPTQIKVLRVAQKSEQLSFGFITELTPKSKFLRKTQK"
     gene            8149..9792
                     /locus_tag="VIG33_13020"
     CDS             8149..9792
                     /locus_tag="VIG33_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8271805.1"
                     /translation="MKSLIIKAPIIPVFIMGVFFASVSALAAEPEFVPGEYIVKLRQP
                     LSSFENKMDVLGQELAAYIKSTIPEQNIIVIKRPVFETKASVIKTLAENPNIEVIEPN
                     FIYRASRTPNDPMFGQLWGMSNEGQADSEKHQGIAGIDIGAPQAWDIETGSKDVIIAV
                     IDTGVDYTNPNLAPNMWTNETELKGQAGVDDDNNGVIDDIYGFNTITQGGDPKDDHGH
                     GSHCSGTIGAKGDDSKGIVGVNWNTRIMAVKFLDKNGSGTLENAIKGINYATKMGAKI
                     MSNSWGGGGEAQTLKEAIERANTAGALFVAAAGNESNNNDTNPSYPASYQVPNVISVG
                     AVNNKGQLASFSNYGRNSVHIGAPGVNIVSTTTSGYDSWSGTSMATPHVSGIAGLIAS
                     HDPKLTNLEIKNRIITTARPIAGLRGKVSGGGLVNAYTALTNTIPQPDQDDPSNWQTI
                     NVSVTTKHPYKAKTNETFEVSVPGAKEIALYFSKFQTEPGYDSIQLVNSSGKIIGEMS
                     GNNDGSFSPVIAGDYVKIIFKSDDSVNEYGFDIPKAAWR"
     gene            complement(9839..10714)
                     /locus_tag="VIG33_13025"
     CDS             complement(9839..10714)
                     /locus_tag="VIG33_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY8271806.1"
                     /translation="MNTVEKETGVFESFDKTPIYYEIRGTGEPLILIYGIACLMNHWH
                     FQMEHFSKHYKVIAFDIRGHHKSQPLGNPQNLKVSDLVQDVMGLLEHLNISKAHFAGH
                     SFGAPLLLELFRVKPELVGSMIFINGFSKNPIKGMFGLDVVEPFFYYVKEQYQKSPTL
                     WTTLWRMAIDNPLSMFLAGTAGGFNLKLTHFKDIEVYARGVARMDLRIFLILFEELMK
                     FDGEEILPLVDVPALIISGEKDNVTPQKFQYEFHHKIHGSEFVLVPYGSHCTQLDFPD
                     YINMKIEAFLKEHPL"
     gene            complement(10724..11413)
                     /locus_tag="VIG33_13030"
     CDS             complement(10724..11413)
                     /locus_tag="VIG33_13030"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY8271807.1"
                     /translation="MVLFIEVLAGPQQGSRYKIEPGHKIGRRRGKIPIDDEKISGEHA
                     QFELNEQGHFVLVDLESSNGILTENRRVKRLAMMPGVVFRLGRTDFRVIDITPPEAQD
                     FARVQSWRENLMEKFPVDWVQNRLQEGMGQPLSPSLKLKFIQGLQADESLLLGYGPRR
                     AGANSLDIDLRDPEAPDLAFEIIPGNGLAQIKNLCQNKLSLNNKLVETEILQDGDLIR
                     IGGTLIKVSYI"
     gene            11559..12077
                     /gene="rpsF"
                     /locus_tag="VIG33_13035"
     CDS             11559..12077
                     /gene="rpsF"
                     /locus_tag="VIG33_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY8271808.1"
                     /translation="MELSKETVKRPYEVIVVVHPDATVDEQKELFRKNKATIESFSGS
                     VFSLETWGKRNLANPIGKLKKAIFFHAYFESSTQAVAELERTMRINEKVLRFMHTRLD
                     ERVPLVKHAETFKKGLAESAAREKEREAKMAARKAAFAAGDRGDRGDRGDRSDRGDRG
                     DRGDRGDRFERK"
     gene            12106..12999
                     /locus_tag="VIG33_13040"
     CDS             12106..12999
                     /locus_tag="VIG33_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HEY8271809.1"
                     /translation="MRVKNSPRKFLAIFSISVLLSLLTVVFGAPLMRVLRITYGALAY
                     WLLGIAVMTIFCFIEAPALSIFVGSIWMTLGIYMELERKGLRWTTSALIGILAGSLFF
                     TGAALINLEKLGVYNLAAAETLLRQFITEKVLAMNPGLPLDAGLLVQLIPSAVVTILV
                     VSLGVGLIFERRVFSWLDLPRERMASQLKLLEFRVPDFVIWIAMIAFLLTMENFHVKA
                     FEILGMNIVNVATVLYFFQGLAILEVSLRSFKAGALARTAIYIILVGQLFPVISAIGL
                     IDYWVDFRRRLRKMRLAPKGN"
     gene            13016..13459
                     /gene="rplI"
                     /locus_tag="VIG33_13045"
     CDS             13016..13459
                     /gene="rplI"
                     /locus_tag="VIG33_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY8271810.1"
                     /translation="MKVILQKDVKDLGKVGDLVNVSEGFGRNFLFPRKLAAEATEKRV
                     KEWEHLQRVADAKKKKAIAERQAILAKLTGTTVTFRVAAGENDKLFGTVTTSDISKEL
                     EKAGHSVDRRDIHLEEPIKVLGQHKAIVRMGEGLEAKILIAVERA"
     gene            complement(13559..13750)
                     /locus_tag="VIG33_13050"
     CDS             complement(13559..13750)
                     /locus_tag="VIG33_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271811.1"
                     /translation="MGNTLLEMVILATGLPEEEVRRELHMLMVQHGKRAETLTTDDLR
                     EIMVEYLQDVILAAKEQHS"
     gene            complement(13887..15302)
                     /gene="dnaB"
                     /locus_tag="VIG33_13055"
     CDS             complement(13887..15302)
                     /gene="dnaB"
                     /locus_tag="VIG33_13055"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY8271812.1"
                     /translation="MSARIPPQHLEAEQSIIGGLMLDHEAFDQVSDLIDTADFYKPAN
                     QKIYQAIKELHSKSQPIDIITVTNLLQSRDELESSGGPEYLVSLLDKTISAANIQTHA
                     EIVREKSLLRRLIATSNGLIERAYSQDYADIESLIDQAEAEVLKIGENKAKQGLVGSM
                     EIVKTSIEKIEELFKRKAEITGLATGFKGMDDMTAGLHPGELTIIAARPSMGKTAFSL
                     NIAQHAALRLKKTIAYFSLEMGKESMMMRLLSAEAKVSMGEIRTGRIQDSSWPKLISA
                     AGTLSEAGLFIDDTPGISPFEIRARCRRLKSQHGLDMIIIDYLQLMDLKQKVESRERA
                     VSEISKALKNIAKELQIPVIALAQLNRGVEGRADRRPMLSDLRESGSIEQDADVIMML
                     YRDDYYDKEDQDKQGHAEVIIGKQRNGATGTVKLRFDAKFSRFRDPDLEASGPSTIAQ
                     PRQAPPPMPGKPRNFAPGANS"
     gene            complement(15399..15698)
                     /locus_tag="VIG33_13060"
     CDS             complement(15399..15698)
                     /locus_tag="VIG33_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8271813.1"
                     /translation="MTPNLNSSDNLFVANLQSVSLEKLVKSKLEVLFAQQKEAQVELN
                     GLYNVVIEQVEKPLLELALRAYNGNQVKTAQMLGINRNTLKKKIDNYKIRVKKNS"
     gene            complement(15778..16428)
                     /locus_tag="VIG33_13065"
     CDS             complement(15778..16428)
                     /locus_tag="VIG33_13065"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEY8271814.1"
                     /translation="MLKSSLKINEIFYSIQGETTYVGIPTVFVRTTACNLRCTYCDTK
                     YSYFEGADQSLEDIIQNIRVHKTKYVCITGGEPLLQKPVYTLMETLCDQGYLVSLETS
                     GSKSIAAVDPRVKVILDVKTPDSGAANSFLLSNLDFATPTTEFKFVICSEKDFEWAEE
                     FCRQHDLFEKYVVLYSPSYGKIAECWLAENILLENSSARLQLQLHKYIWSPEKRGV"
     gene            16532..>18166
                     /locus_tag="VIG33_13070"
     CDS             16532..>18166
                     /locus_tag="VIG33_13070"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEY8271815.1"
                     /translation="MNLILKKFRHDILAISFLGFACFLGMALASFNPIDPSLNSLGQG
                     LRASNYCGIVGSFLSDLLYQFFGLTSWLLVVGFMKLAYAGFCREDIDFKNIRLIWGFL
                     LIINLSSLLSLYLPEKKVFTHQIYLGGLLGLGVSQALLRAFNYAGVQVVLWSLSALLI
                     VFYSEKTVKELLRLPAEVYERVAGWGVAARIKKMFFFKVPKKKGKTAIEGVSGKGFFN
                     IKDESFVEKSEEKSAPFPKSLVPKQLEFEGDLDEDEALTEDEDGPQATQTSAKRKVVL
                     KTRVPQKVANWQMPKLSLLDDPPATRIKIDEKEIRRKADLLVDKLKQFNIEGQIVAVK
                     PGPAVTMFEFKPNADVKLSKITELADDLSLALSSESLRIIAPIPGRDVVGIETSNTQR
                     ETVYLKDMLAEDNFWSEEMKLPIALGKQANGEPRIVDLRKMPHLLVAGTTGSGKSVFT
                     VSSITGMLFKHSPKTLRLILVDPKQVDLAAFAQVPHLIMPPVTDPKKAVSALRWAVKE
                     MEKRNKSMSKFGARKLEEFNEMVAKLSSEQISEHEKF"
BASE COUNT         5159 a         3695 c         4009 g         5303 t
ORIGIN      
        1 caggggcttt aaacaaaggg tcaaagaaat ctatattgcc gatttccacc cagcccacca
       61 gcccacctgc ctttgcttct ggcgcaacag agtaagtctt agcaagttct gcaaaatctt
      121 ttgatttaac ctgagacttt aaaaggtcgg ccttcccttg ttcatctgtg acgatttggc
      181 gaacataaat tctttctttt tttctaaatt gctctttatg ttcatcatag tagtgtttaa
      241 tttcaatctc actcaggggt ttaatccgtt cgttgagctt tttgaaaacg gctcgttcaa
      301 taagggtata acgtagattt tctcgccatt cagaaaagga aatattttcc tgagcaagaa
      361 gtctacgaaa agacaaatca tcaggataat tggctcgata tttatcgacc tctttatcta
      421 tatcattttc attaataacg agactgttag cgcgggccca atcgagagtc agagatttta
      481 caagaaatga gcgaagaagt tgttctttcg ttctatgaac ggtagaggga tctttcgccg
      541 ttaaagcatc aaggtctttg agtttcctag cgagctggtt agaaaactct ttagctgtta
      601 atacatgatc attgaccttg attacgggtt tggttgagag cttctgatag ttcgatgtgc
      661 atccgttcac aactaacaat aaattcaaaa taaaaagccc cgtcacggcg gggctcttaa
      721 taaacctcat gggtaagaat cccttatttt aaaaggcttg gatttgtttt aatcgcgtag
      781 ccttttttaa gtttttcaaa gtattcatta aaagcttgtt ttcttttttc atcaaagaca
      841 gcagctctaa cttggcgttt attggcatct tcatagcttc tacggcctgt aattttaata
      901 acgtggaaac caaatggagt ttcaaccaag cctttgatct cattaacttt tccgcttaag
      961 gccgcttcgt aatatgaagg aacgagggtt actctggact gccaacccac atctccgccc
     1021 gcctgcttgc ttagtggatc gtcagaatat agcttaacca gctcttcaaa aggtcgctta
     1081 gattttcgta cttcttcata gatttcattc gcacgcttct gggcttcagc gatttgttca
     1141 cgcgtcgcac cggccttaaa ttcaataaga atatggcttg tacgaatctc tggattgttt
     1201 ttgtaccaag ccttcatttc aggttcagaa acctgaattt tttgaacttt ttggccaatg
     1261 tccttttcaa gaagggcctt gtaagtttct tgttgaatac gctcaatgac aattggatct
     1321 ttctctaaat tacgcttagc tgcctcttgc aatcccactt catagcgaac catatcttca
     1381 agaaactgta tctttgtcgg tgggttcaaa gtttgtgact taatttcatt aaatttttta
     1441 ttaaagtcat cgagggttat tcttttattg ccgacagtgg cgacaacttc agaagctttt
     1501 gattgagcat acgacaatga agtcgagaga agaaggacgc taaaagcgtt aataattgca
     1561 gtcaaattca tacctcaaaa gtcctttaaa aagttgacga cataccacat taaaacgcta
     1621 gcattcggct taagattcag caatgacttt aagggcttgt tgttttgcgc gatgtagcaa
     1681 attttcgagc tgtgtactaa attttgggtc gcgtggatcc tggtctttcg tcatagcagg
     1741 aagcggtcca tgccattgaa taatgatttt agaaaatggt ttgggcaaga aggttttatt
     1801 ccaagcattt ggaaaatgaa tcgcgctgct tacactaacg ccgcaataat atatagggca
     1861 gttcaaaagg cgtgaaagct caaagattcc aggcttaact ttgtaaatgg gacctttagg
     1921 gccatccaca gcaaaactgc aattatgacc ttcacgcata agtcgtatga gacccttgag
     1981 tccgccgact ccgccgcgac ttgaagatcc ttgagtagtt ttggcaccca acaaacggag
     2041 aatgatagac atcatttgtc catctttact ttgggaagac atagttgcaa ttcgatagcg
     2101 acgaacaagt tgaattaaag cgatttcgtc accatgccag tgggctagta aaaaaggatt
     2161 tttgtccttt atgttttttt gcagacactc cggctcaatt aactgcactc tccaggtaaa
     2221 agagaaaata cgataaataa gccagactag aaaccctaat atatattttc tcaaaggact
     2281 tagtgcttaa gaactttttt gaattcttca gtgagtttgg gaattacatc aaagagatct
     2341 ccgacgattc cgtaagtcgc cttttggaag attggagctc caggatcgtt gttgattgca
     2401 acgataactt tgctaccact catgccagcg agatgttgaa ttgcgccaga aacgcctaca
     2461 gcaatgtata aggtaggtgc aactgtttta cccgtttgac ccacctgcat gccgtgagaa
     2521 acccaaccag cgtccacaac agcgcgtgaa gcaccgacag tggcacccaa aacatccgcc
     2581 aactcattaa gaagcttaaa gttaccagct tctttaagtc cacgtccgcc tgaaataata
     2641 atattagctt cagttagatc cagcttttcg gaggcacctt taacgatttc cttaatcacg
     2701 gttttaagat caggcttggt tagcgcgtgc tcaacaagaa ccatcgtttt agaagtatcc
     2761 gcagcggcaa ctgggagttg atttgctcgc atcaatacaa ttttaagagg actattttca
     2821 aagtttgcaa ttgcaaaagc tttacctgag tacattggct ttttagcagt gatactgtcg
     2881 ccagagatac tgagttcaac gcaatctgat accacacccg ttttaagatg agctgcaatc
     2941 cgtggaaaaa gatccttagc ggtacttgtt gcggaagcaa gtaaaatttg tggttgggtc
     3001 ttttctaaaa cggaagatag tgcagataaa taaagctcag gattataagc atcaagattc
     3061 gagtctttga ttacatgaac ttcttgagcc ccgtggtagc ccatttcagt ggctgctttt
     3121 tcagcgccag tgccaacgac catgccaata accgtgtttc cggatttttg agctgcctgc
     3181 aaaagctcca tagctccacg cttaagagtc ccatttttat gttcggcaaa tacaaatact
     3241 ttagacattg aatcctctat aatactttgg cttcatcacg aagcagttgt acaagttcag
     3301 ccacctggtg agcagaatct cccatgagca ttttcacaga aggtttttct gctggaagac
     3361 tgagttggga gtatttaact ttaatgtctg tggcaggaat atttaaagaa gcaaattcga
     3421 tttcttttaa tacttttttc ttggccttca taataccagg caggcttgcg taacgagggg
     3481 tatttaagcc tttattggcg ccaataatcg ctggagctgt catattgata atttctttcg
     3541 ctccgccttc gatatcacgc tctacgctga caccttcagg agaccaagca aacttagaga
     3601 ccacagtcgc gtgaggaatt cctagaaact cgccgagcat ttggctaacc gaggatgcgt
     3661 tatcatcaat agccaattta cccgtgaaaa ttagacctgt gtctttttcg gatttaatga
     3721 cttcagccag ggctcgagct gtcgagtagc aatcaatgtc ttcgggagca ttaatgacga
     3781 ttccttcatc cgcacccatc gctagagcag ttcttagtgc ttcagaaact cgctttgaag
     3841 gtcctacact taagacaaaa acttgagcgc ctgcgctggc attagcttcg cgatatttaa
     3901 tagcctcttc gacagcgtat tcgtcataag gattaatcac ccatttgatg cctgttgtgt
     3961 cgatagaagt ggcatcgggg ttgattttaa tctttgtttc ggtgtctgga acttgcttaa
     4021 tacaaacaaa aattttcatg tgaacctcgt ggggtctttt tatcttaaag gggacctctg
     4081 gcaaacctaa aagtcttaag taacatgacg cgaaaaccca tagaagcgcc gtggacaaaa
     4141 tgcacatggc gggagtaaaa ggtattgaaa ctcaactgaa aattagtagc ataaaagagg
     4201 tttactgtgg ctcttatagc gtctcatggt tttttgagat ctacgatcgg caaaaaatat
     4261 ctcatggggt tggcaggact tgtatggacg ggttttgttc ttgttcatat ggctggcaat
     4321 cttcttatct ttgtgagtcc agatgcatac aatgcatacg gacatgcaat cactagcggc
     4381 aacattgttt acattgctga ggctgttctt atcttagcct tgatcgtgca tgttgggctt
     4441 gcgtttagac tgactatgga aaaccgcgcg gctcggtcca gtacttatgc tgtaaaggca
     4501 aaaggtgaga aatctgcgac tctggcctct cagacaatgg gaatccaagg gagtttgatt
     4561 ttagcattca ttattttaca tattgcgact tttaaatacg gaacacacta cgatacgacc
     4621 gtaaagggcg ttgtcattcg cgatctttac agattgattg cagaagtatt ccaacaacca
     4681 ggttacgtcg tctggtactt ggtcagttta gtggttttag gctttcattt aagtcatggg
     4741 gtaggttcag ttttccaatc ctttggttgg atgaatgggc gtcatcaacc ccttttaaag
     4801 aagatcagct atctttatgc tggcatagtg gcattaggat ttatttctca accaatctat
     4861 attttcctca tcgcaggcaa aggttaaggg agtcgagcaa tgtctaaaat attagatggc
     4921 aaagttccag atggagcgat caataaaaaa tggacagatc acaagttcca catgaaactt
     4981 gtgaatccag cgaataaacg aaaacattca gtgattgtag taggaacggg acttgcgggt
     5041 gccagtgccg cggccgcaat gggtgaactc ggttataaag tgaaggcttt ctgcgtgcat
     5101 gaatctccgc gccgtgccca ctctgtggcc gcccaaggag gtattaatgc cgcgaaaaac
     5161 taccaaaatg atggagattc catctatcgt cttttttacg acactgtaaa aggcggagat
     5221 ttccgggctc gcgaagccaa tgtgcatcgt ttagccgagg tctctgtaaa tatcattgac
     5281 caggcagtgg cctcaggtgt gccattggct cgcgaatatg gcggtctgtt agacaatcgt
     5341 tctttcgggg gcgcgcaagt ttctcgaact ttttatgctc gcggacaaac cgggcagcag
     5401 cttcttttag gcgcttactc tgcgatgatg agacaagtag atcttggcac tgttgaattg
     5461 aacacacgca gagagatgct agatcttgtc gtggttgagg gtcatgctcg tggtattgtt
     5521 gttagaaatc ttcataccgg tgaaattgaa tctcatgagg cagatgctgt cattatcgct
     5581 tctggaggtt actcaaacgt tttctatctt tctacgaatg caatggcttg tgctgtaacg
     5641 gcggcgtgga aagctcacaa acgtggtgcc tattttgcaa atccttgtta tactcagatc
     5701 catccaacct gcatccccgt ccatggagaa aatcagtcaa aacttacttt gatgtcagaa
     5761 tctctccgta atgatggacg tgtttgggtt cccaaagcaa aaggcgataa acgtcatcca
     5821 aacgaaattc cagacgacga aagagattat tatcttgaac gcgtctatcc tagtttcggc
     5881 aatttagctc ctcgagacgt ggcttctcgt caagcgaagt atcgctgtga tgaaggtcat
     5941 ggtgtgggcg attctggaaa agcagtctac cttgattttc gtgatgctat tgttcgttta
     6001 ggtgaggcaa aagtctcaga acgttatgga aatttatttc aaatgtatga aaaaatcact
     6061 ggtgaaaacc cctataaaca gccgatgagg atctatccag ctccgcatta tacaatgggt
     6121 ggtctctggg ttgattataa tctagaatcg acaattcctg gtctttttgt agcgggagaa
     6181 gcgaacttct cagatcacgg tgcaaatcga ttgggagctt cggcgttgat gcaaggtttg
     6241 ggagatggtt attttgttct tccgtacacc atcagtaatt atattgctag tacgaaactg
     6301 aataaagtat caactgcaca tgcggctttc aaagaggccg agacttcagt taaaaatgaa
     6361 atcactaaaa ttatgagtat taaaggtaca cgtacggtgg aaagcttcca taaagagctt
     6421 ggacatatta tgtgggaata ctgcggaatg gctcgtaatg aagctggatt gaaaaaagct
     6481 ttagaattaa ttccgaaact tcgtgaagag ttctggaaaa atgtgcgtgt acccggagaa
     6541 gtcaattacc tgaattctga gcttgaaaaa gcgggacgtg ttgcggattt cttagagctg
     6601 ggcgagctga tgtgtcggga tgctcttgaa cgacgtgagt cttgtggtgg tcacttccgt
     6661 gaggagtttc aagaagacgg tgaagcaaag cgtgatgacc aggacttcag ccacgtagct
     6721 gcgtgggaat ggaatggacc aaatcagccg caaactcgcc ataaagaaga actaaaattc
     6781 gaaaacgtta aacttgcaac tcgtagttat aagtaagggt gaaccatgag tgaactgaca
     6841 aaaaaaatta atttaacact ccgagtttgg cgccagaaaa gtccgaagga tcaaggtggc
     6901 tttagagaat acgcggcaaa aaatgtttcc actgatgcct cttttctgga aatgctagat
     6961 gcggtgaatg aagagcttat caataaagga gaagacccta ttgcgtttga ccacgattgc
     7021 cgagaaggta tttgtgggag ctgtggtttt atgatcgatg gacaagccca tggaccgcaa
     7081 aagtctacaa ctgtttgtca gcttcacatg cgagctttca atgatggcga agttttaact
     7141 ctcgagccat ggcgagccac agcttttccg atcgttaaag atttgctaac cgatagaaca
     7201 gccttggatc gtattatcac ctccgggggt tatatttctg caaatactgg aagtgcaaga
     7261 gatgctaata gcattttgat tccaaagcac gatgctgatc atgcctttgg tgcagctgct
     7321 tgtattggtt gcggagcctg tgtggcttct tgcaagaatg cttcggcggc acttttcacg
     7381 tcagcaaaaa tttctcattt ggctcttctt ccgcaaggcc aagtagaaag aaaaattcgc
     7441 gcggaacgca tggttgcgca aatggatgcg gaaggttttg gcgcttgttc aagcacaggt
     7501 tcttgttcag cagcttgtcc gaaagatatc agcctggtga atatcgctcg tatgaaccgg
     7561 gaatttttta aagcaactat tcttaaagag gatgaagctg ataccggcgg cggattctaa
     7621 ataaattaat atttaaacgt agctgtataa ataaaaaagc actggactaa agaccagtgc
     7681 ttttttcatt tctcaaccaa cgtacgagtt cccccgtctt gtcgttaaga atcttgaaat
     7741 aagtccgaag aagaactcac gaggacgaaa tgaaaaagct atcggagttg atggttgaat
     7801 tggggtttga tcctgaaggt tcttccgctg ttaaagaagc gttcattaaa tatctcatta
     7861 aagcgagtga gggcatgagt gttctccctc ccacggaaaa tgcaataatc aaagcaaatc
     7921 caacccagat caaggtccta cgagtagcgc agaaatctga acagttatcc ttcggattca
     7981 ttacagagct aacaccaaag tccaaatttt tacgaaaaac tcaaaaataa ataatatctt
     8041 tttccaaggc attttgcggt tggtaccatt ttggaactag gtcgatgaag agtttttcta
     8101 aatattttat tcgcatcttt ggccgttacc gaaatccaag gaggatttgt gaaaagtctt
     8161 ataataaaag ctcccattat tcccgttttt ataatgggcg tattctttgc ctcagtctcc
     8221 gcacttgcgg ctgagccaga attcgtaccc ggtgaatata tagtaaaatt gagacagcca
     8281 ctgagttcct tcgagaataa aatggacgtt ttaggccaag aacttgctgc ttacattaaa
     8341 agtacgattc ctgaacaaaa tattattgtc attaagcgcc cggtttttga aaccaaagct
     8401 tcagtaataa aaacactggc agaaaatccg aacattgaag tgatagagcc gaacttcatt
     8461 tatagagcta gccgcactcc taatgatcca atgtttggtc aactctgggg tatgagcaat
     8521 gaggggcaag cggactctga aaagcatcag gggatagcgg gtatagacat tggagcccct
     8581 caagcttggg atatcgagac aggttctaaa gatgttatta ttgcagtcat tgatacgggt
     8641 gtggattata caaatccaaa tttagctccg aatatgtgga ctaatgagac tgaacttaaa
     8701 ggtcaagctg gagtagatga cgataacaat ggtgttatcg acgatattta cggattcaat
     8761 actatcactc agggtggtga cccaaaagat gaccatggtc atggatcaca ttgctctggc
     8821 accattggcg ctaaaggtga tgacagtaag ggtattgtag gcgtgaattg gaatactcgg
     8881 attatggcag tcaagtttct tgataagaat gggtcgggaa ctctcgaaaa tgcaattaaa
     8941 ggtataaatt acgcgacaaa aatgggtgcc aaaatcatgt cgaattcttg gggcggtggt
     9001 ggcgaggcac aaacccttaa agaagccatc gaaagagcca atacggccgg tgcacttttc
     9061 gtagcggcgg caggtaatga atccaataat aatgatacga atccttctta tccagcctca
     9121 tatcaagttc caaacgtaat ttcagtaggg gccgtaaata acaaaggtca attagcctca
     9181 ttttctaatt atgggagaaa ctcagttcat atcggtgctc ctggggtaaa catcgtaagt
     9241 actacaacct cagggtatga ttcctggtct ggaacttcaa tggcgacacc tcatgtatct
     9301 ggaattgcag gtttgatcgc atctcatgat ccaaaactta ctaatcttga gattaaaaat
     9361 cgtatcatca caactgctcg tcccattgct ggccttcggg ggaaggtatc cggtggtggg
     9421 ttagtaaatg cttatacggc actaactaat accattcccc aacctgatca ggatgatcca
     9481 agcaattggc aaacaataaa tgtttcagta acaacgaaac atccatataa agcgaaaacg
     9541 aatgaaacct ttgaagtgag tgttcctgga gcaaaagaaa ttgctttgta tttttcaaaa
     9601 tttcaaactg aaccaggcta tgattctatt cagctggtca attcaagtgg aaaaatcatc
     9661 ggcgaaatgt cgggaaacaa cgatggtagt ttctcgccgg tcattgcggg tgactacgta
     9721 aaaattattt ttaaatcaga cgattctgta aatgaatacg gtttcgacat tcctaaagca
     9781 gcttggagat aagctcctac gaagaagtct ttgaaataat ccaatagcgt ttttgtcttt
     9841 aaagaggatg ctctttcaaa aacgcttcta ttttcatatt aatatagtcg ggaaaatcta
     9901 gttgcgtgca atgtgaccca tatggaacta atacaaattc actcccatgg attttatgat
     9961 gaaattcata ttgaaatttt tgaggggtta cattgtcctt ttcgccggaa ataattaagg
    10021 ctggaacatc gaccagaggg agaatttctt ccccatcgaa tttcatcaat tcttcaaata
    10081 aaatcaaaaa aatacgaaga tccattcggg ccacaccgcg agcatagact tcaatatcct
    10141 taaaatgggt gagttttaaa ttaaaacctc cagcagtccc ggctagaaac attgagagtg
    10201 gattatcaat tgccattctc cacaaagtag tccacagagt gggagatttt tgatactgct
    10261 ctttgacgta ataaaagaat ggttctacta catctagacc aaacatcccc ttgatcgggt
    10321 ttttagagaa gccattaatg aaaatcatag agccaacaag ctcaggtttg actcgaaaaa
    10381 gttccagcaa tagtggtgcg ccaaaactgt ggccagcaaa gtgggctttc gaaatattta
    10441 aatgttcaag taaacccata acatcttgaa ccaaatcaga aacctttaaa ttttgtggat
    10501 tgccgagagg ttggcttttg tgatgacccc gaatatcaaa tgcaatgact ttgtagtgtt
    10561 ttgaaaaatg ctccatttga aaatgccagt gattcataag acaagcgata ccgtagatga
    10621 ggataagagg ttcacctgtt ccgcgaatct cgtaataaat cggcgtctta tcaaagcttt
    10681 caaaaacgcc agtttctttt tcgactgtat tcatgcacaa cccttaaata tagctcactt
    10741 taattaaagt gccaccgata cgaattaaat ccccatcctg aagtatttcg gtctctacaa
    10801 gcttattatt gagactcaac ttattttggc agaggttttt tatctgagcc aagccgttac
    10861 cgggaataat ttcaaatgca aggtctgggg cttcgggatc gcgaagatca atatccaaag
    10921 aatttgcccc tgccctacgg ggcccatatc ccaagagaag agactcatcg gcttgaagtc
    10981 cttgaataaa ctttagtttc aaagaaggag ataacggctg ccccatgcct tcctgaagcc
    11041 tattttgtac ccaatccaca ggaaattttt ccataaggtt ttcacgccaa gactgcactc
    11101 gtgcaaaatc ttgtgcttca ggaggtgtta tatcaataac gcgaaagtcc gtacgcccca
    11161 gacgaaaaac gactccaggc atcatagcta gtcttttgac tctgcgattc tccgttaaaa
    11221 taccatttga tgattctaaa tcgaccaaaa caaagtgccc ctgctcgttt aattcaaatt
    11281 gggcatgttc cccagagatc ttttcatcat caatagggat ctttccccgc cttcgtccga
    11341 ttttatgacc aggttcaatt ttatatcttg atccttgttg gggccccgca aggacttcta
    11401 tgaataatac catgtaatta ttgtaaggga ctttttaaaa agcttcaata ctctcgcttg
    11461 tgcttatcct ttaggcgtgc taaatcaaca accctaagtc aaaaagtcat ccttgttcct
    11521 gacattgggt cagggactat aaaccgaaag gatcgaaaat ggaactatct aaagagactg
    11581 taaaacgtcc ttatgaagtc atcgttgtcg ttcacccaga tgctactgta gatgaacaaa
    11641 aagagctttt tagaaaaaat aaagctacta ttgagagctt ttcaggctct gtattctctt
    11701 tggagacctg ggggaaacgc aatctagcta atcccattgg caagctgaag aaagcaatct
    11761 ttttccatgc ttacttcgaa tcaagtactc aagcggtcgc tgagcttgag agaacaatgc
    11821 gtattaatga aaaagttctt cgttttatgc acactcgtct agatgagcgt gtacctttag
    11881 tgaagcatgc agaaaccttc aaaaaaggtc ttgctgaatc tgcagctcgt gaaaaagaac
    11941 gcgaagctaa aatggcggct cgtaaagcgg catttgctgc tggagatcgc ggcgatagag
    12001 gcgatcgtgg tgacagaagc gaccgcggag atcgtggaga tcgcggcgac agaggcgatc
    12061 gtttcgaaag aaagtagtct ctgatgaggc tcaagggtca gaagcatgag agttaaaaac
    12121 tccccccgaa agttcctagc catattttcg atttcagtat tgttgtcttt gctgactgtg
    12181 gtttttgggg ctcctcttat gcgcgtttta agaattactt atggcgcact tgcttattgg
    12241 ctcttgggaa tcgcagtgat gactattttc tgttttattg aggctcctgc tctttcaatt
    12301 tttgttggtt cgatttggat gactttagga atctacatgg aacttgaaag aaaaggactt
    12361 cggtggacaa cctctgcact tattgggatt ttggcgggca gcttgttttt tacaggtgcg
    12421 gcgttgataa atctcgagaa gttaggagtt tataatcttg ctgcggctga aacccttcta
    12481 aggcaattca ttacggaaaa agtgctcgcg atgaatcctg gtttgccctt agatgcgggt
    12541 cttctggttc agctcattcc ttcagcggtc gtcaccattt tagtggtatc cctcggtgtt
    12601 gggttgattt ttgagagaag agttttttcc tggttagatt tacccaggga gcgaatggcc
    12661 tctcaactca aattgttaga gtttcgcgtt cctgattttg ttatctggat agcgatgatt
    12721 gccttccttc taacaatgga gaatttccat gtgaaggcgt tcgagattct tgggatgaat
    12781 attgtgaacg tggctacggt cctttatttt ttccaaggtt tagcaatatt ggaggtaagt
    12841 cttagatctt tcaaagctgg tgctttggca agaactgcga tttacataat tctggtcggt
    12901 caattgtttc ccgtaataag tgcaattggc ttgattgatt actgggttga tttcagaaga
    12961 cgtctcagaa aaatgagatt ggctcctaaa ggcaactaga aatagcgagg gtcaaatgaa
    13021 ggtaattctt caaaaagatg taaaagattt gggcaaagtc ggagacttag tgaatgtttc
    13081 cgaaggtttc ggtcgtaact ttttgtttcc cagaaagtta gctgctgaag ctactgaaaa
    13141 acgcgttaag gagtgggaac acttgcaacg cgttgctgat gctaagaaga aaaaggcaat
    13201 tgctgagcga caagcaattt tggcaaaact cactggcact actgtaacat tcagagtcgc
    13261 cgctggggaa aatgacaaac ttttcggtac agtaacaact tcagatatct ctaaagagtt
    13321 ggaaaaagca ggtcattcag ttgatcgtcg cgatatccac cttgaagagc cgatcaaagt
    13381 tcttggtcag cacaaagcaa tcgttcgtat gggcgaaggt ttagaggcta agattttgat
    13441 cgcggtagaa cgcgcttaag actcataaac gaagaaaaca aaataatatt tggaaaggga
    13501 ttctggtaac gggatccttt ttttatctac cagtaaggct gactaaaaga tttcttattt
    13561 aagaatgctg ctctttggct gccaatataa cgtcctgtaa atactccacc attatttccc
    13621 gaaggtcgtc cgttgtgagt gtttcggctc tctttccatg ctgaaccatc agcatgtgaa
    13681 gctcgcgacg aacctcttcc tcagggagcc cagtcgcaag aatgaccatc tcaagaagtg
    13741 tattccccat tcgagcctcc gtgctgtgtg ggttgtttaa ttgacaaaat cattccacca
    13801 atcggttagt cgaaactcaa ctgattggta gcgctttcaa aggatttagt ccaaggtctg
    13861 gaccatattc tagatttggc ttttaattaa gaattagcgc ctggagcaaa atttcgaggc
    13921 ttccctggca ttggaggagg tgcttgacga ggttgtgcta tagtagaggg gcctgatgct
    13981 tccaaatccg gatcgcggaa tcgagagaac ttagcatcaa atcgcaactt cactgtgcct
    14041 gtcgcaccat tacgttgttt accaataata acctcggcat ggccttgttt atcttggtct
    14101 tctttatcgt aataatcatc acgataaagc atcataataa catcggcatc ttgttcgata
    14161 gatccagatt cacgtagatc tgaaagcatt ggacggcggt ctgcacgtcc ctccactccc
    14221 cgatttaact gagcaagggc aatcactgga atctgcaatt ctttggcaat gtttttcaaa
    14281 gctttggaaa tttcagagac cgcacgctca cggctttcta ctttttgctt taaatccatg
    14341 agttgaaggt agtcaatgat gatcatatca agaccgtgtt gcgattttaa acggcggcat
    14401 cgcgcacgga tttcgaaagg actaattcca ggagtatcat caataaaaag cccggcttca
    14461 ctcaaagtgc cagcagcgct aataagtttt ggccaagatg aatcttggat tcgacctgta
    14521 cgaatctctc ccattgaaac ttttgcttcg gcagaaagaa gacgcatcat catcgactct
    14581 ttacccatct ctagagaaaa gtacgcaatg gtctttttaa ggcgtaaggc cgcatgctga
    14641 gcgatgttca agctaaaggc agtcttaccc atggagggac gagcagcgat gatggtaagt
    14701 tcgccagggt gaagaccggc ggtcatgtca tccatgccct taaagccagt tgctaaaccc
    14761 gtgatctcag ctttgcgttt aaaaagttct tcgattttct cgatgctcgt tttaacgatt
    14821 tccatcgatc caacaaggcc ttgctttgct ttgttttcgc caattttaag aacctcagct
    14881 tctgcttggt caatgagact ttcaatatcg gcatagtctt ggctataagc acgctcaatc
    14941 aatccattgc tggtggcaat caaccggcga aggagagatt tttcccgcac tatctctgca
    15001 tgcgtttgga tattggcagc agaaattgtt ttgtccagga ggcttacaag atattcaggg
    15061 ccaccagaac tttctaactc atcccgtgac tgaaggagat ttgtcaccgt aatgatatca
    15121 atcggctggc ttttactatg aagttcttta atggcctgat agattttttg attagcgggc
    15181 ttgtagaaat ctgccgtatc gatcaagtcg gaaacttgat cgaaggcttc gtgatctaac
    15241 attaaaccac caataattga ctgctctgcc tctaaatgct gaggcgggat tcgtgcgctc
    15301 ataacccctc cggagacacg ttaattattt aatggatgcg gaacaaaacc caagtagtta
    15361 agcatgttga tccgccgatg ttaatcattg cagaagtact agctattttt tttaacacga
    15421 attttgtagt tgtcgatttt cttttttaaa gtgttacgat tgatgcccag catttgagca
    15481 gtttttactt gatttccatt ataagcacgc aatgcaagct ctagaagtgg tttttccact
    15541 tgctcaatca caacattata tagaccattc aactcgacct gagcttcttt ttgttgagca
    15601 aataatactt cgagcttgct cttaaccagc ttctctaagc ttacagattg aagattagcg
    15661 acgaaaagat tatcagagct gttcaggttc ggcgtcatgg aactcccaat ttagaaattt
    15721 aaaattagct gaaaaattaa ctgaaatcat aagcccgggc tgtatacctg cttttgacta
    15781 cactccgcgt ttttcaggag accaaatata cttatgcagt tgtaattgca accttgcaga
    15841 tgaattttct agcaaaatat tttctgccaa ccagcattca gcaattttgc catatgatgg
    15901 gctgtataaa accacatatt tttcaaataa atcatgttga cgacagaatt cttctgccca
    15961 ctcaaaatct ttttctgagc aaataacaaa cttaaattca gtagtaggag tggcaaagtc
    16021 aaggttactc aacaagaagg aatttgctgc accactatct ggagttttta catcaagaat
    16081 gacttttact cgaggatcca cggcggcaat gcttttagag ccactcgttt ctaatgaaac
    16141 caaatatcct tgatcacaaa gcgtttccat taaagtatat acaggttttt gcaagagagg
    16201 ctcccctccg gtaatgcaaa catattttgt tttgtggact cgaatatttt ggatgatgtc
    16261 ttcaagggat tggtccgcgc cttcaaagta ggaatatttt gtatcacaat aagtgcagcg
    16321 aagattacaa gcagtggttc ttacaaagac cgtgggaatc cctacataag tggtttcgcc
    16381 ctgaatacta tagaaaatct catttatttt tagtgaactt ttaagcatct ctggaccctt
    16441 gtcttgggag tatgtaccta gaccatatcc taaggtcaag gctgctgacc caaagctttc
    16501 cctattgtaa tatgtcctgt aaatttttga aatgaacctg attttaaaaa aattccgtca
    16561 cgacatcctt gcaatctcat tccttggttt cgcgtgtttt ttaggaatgg ccctcgccag
    16621 ctttaacccc atagatcctt cgctaaactc tttaggacag ggtttaaggg cgtcaaacta
    16681 ctgcggaata gttggtagtt tcttgtcaga tcttctctat caattttttg ggctgacttc
    16741 ttggctcttg gtggtgggtt tcatgaagtt ggcttatgcg ggattttgcc gcgaggacat
    16801 tgattttaag aacattcgtc ttatctgggg ttttctgcta attatcaatc tttccagttt
    16861 attgtctttg taccttccag agaaaaaagt ttttacacat caaatctact tgggtggatt
    16921 gcttggctta ggggtgtcgc aagctcttct cagggccttt aattacgcgg gtgtgcaagt
    16981 ggtattatgg agtctgtcag cgcttcttat cgttttctat tctgaaaaaa cggtgaaaga
    17041 actccttcgt ctgccagctg aggtctatga gcgcgttgct ggctggggtg tggcggctcg
    17101 aataaaaaaa atgttttttt tcaaagttcc taagaaaaaa gggaaaacag ctattgaagg
    17161 tgtctctggc aagggcttct tcaacatcaa agatgaaagt tttgtggaaa agtctgagga
    17221 aaagtcagca cctttcccca agtctttagt accaaaacaa ttggaattcg aaggggatct
    17281 tgatgaagat gaggctttga cagaagacga agacggacct caagccactc aaacttccgc
    17341 aaagcgaaaa gtagttttaa aaactcgggt cccgcagaaa gttgcaaact ggcaaatgcc
    17401 aaaactttcg ttgctggacg atcctcctgc cacgcgcatt aaaatcgacg agaaagaaat
    17461 tcgcagaaaa gccgacctct tggttgataa attaaaacag ttcaatatcg aaggccaaat
    17521 cgtggcagtg aaacccggac ctgccgtgac catgtttgag tttaagccta atgcggatgt
    17581 taaactgagt aagatcacag aactcgcaga cgacttgtcc ttagcgttaa gcagtgagtc
    17641 tttacgcatt attgcaccca tcccaggtcg ggatgttgtg ggtatcgaga cctcgaatac
    17701 tcagcgtgaa acagtttatt tgaaagatat gttggcagaa gataattttt ggtctgaaga
    17761 aatgaaattg ccgatcgctc taggtaagca agccaatggt gagccgcgca tcgttgatct
    17821 tcgtaagatg cctcaccttt tagtggcagg gacaacgggt tcgggaaagt ctgttttcac
    17881 agtgtcttcc attacgggta tgctgttcaa gcactcccct aaaaccttac gcttaattct
    17941 ggtcgaccca aaacaggtgg acttagcggc ctttgcccag gttccgcatt tgattatgcc
    18001 tccggtgacg gatcctaaga aagcggtctc agcacttcgt tgggcagtaa aggaaatgga
    18061 aaaacgcaat aagtccatgt caaagtttgg agctcgtaaa cttgaagagt tcaatgaaat
    18121 ggtggcgaag ctttcgtcgg agcaaataag tgaacatgaa aaattt
//
LOCUS       DATBTE010000350         3004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1072213, whole genome shotgun sequence.
ACCESSION   DATBTE010000350 DATBTE010000000
VERSION     DATBTE010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072213"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1089)
                     /locus_tag="VIG33_13075"
     CDS             complement(82..1089)
                     /locus_tag="VIG33_13075"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEY8271816.1"
                     /translation="MPIPPPQDPLKLAKSSAQAPVPLDLSRVSNQEILFRLERLSRSE
                     RKITHLILCHINEVELRKLYSELGYDSLYKYLTQHLHYGGSAAYDRMQAARVLKISPD
                     IAPKIEEGSLNLSQLVKVEQSLKQEQKSGKALSPKGTQELLGKLENKSGSETDKILAC
                     ELNQTPKTHQKLKPQKDESVRMEFTLTQEQYEILKKAQNLISHSVPENDLAKVITYLA
                     QAYIQKMEGSKKNKAKPEAPSLTPSRTEMPLKVYKTLRQKRKFISLKIRREVFAKAHH
                     CCEFINPLSGKRCTSKFQLQLDHITPLACGGTDELTNLRVLCGVCNRKEAQRWGLERP
                     L"
     gene            1672..2451
                     /locus_tag="VIG33_13080"
     CDS             1672..2451
                     /locus_tag="VIG33_13080"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY8271817.1"
                     /translation="MFANKSSALLAVVLGGVFLGAFYQNMSPVEFAALNMPPIDEGAR
                     RVQARELLGTYYGGSFAQSFEGQKDMNYLIYRKLESSFSSKWKKWLPQMTEELIRECQ
                     KRDMDPVFILAIIQTESQFNVYAKGTSGEIGLMQVLPQTAAWMAKKYHLPWQGPNSLY
                     DPITNIKFGITYFAHLRSQFESRAHHYVPAYNMGPKNLRRIARSIVSMGEDERDLLDG
                     IYGSKVMKNYQMIYQQMVQGNKSLPNQWAQDASQEELVRTR"
     gene            2586..>3004
                     /locus_tag="VIG33_13085"
     CDS             2586..>3004
                     /locus_tag="VIG33_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271818.1"
                     /translation="MRAGVMSVLVALSAVMAQAAGRQEASEVCTSMSFDSDRNYCISA
                     ISKYDYFDQGAINLCLGMSFDSGKKECISNIGNKTYDPYELTNCEKNDFDSSKNQCLK
                     GAGRPIQGHVGCLNNQDIIFQLQILEGMVYRGENGRAR"
BASE COUNT          798 a          573 c          740 g          893 t
ORIGIN      
        1 agtccctgcc ttccggctgc atttcaactt actccaaaaa ctgtaatcca aaactgtttt
       61 tattttacag aacatttcat tttacaaagg ccgctcgagt ccccacctct gagcttcctt
      121 tcggttacag actccgcaaa ggacgcgaag gttggtgagt tcatccgtgc ctccacaagc
      181 cagaggtgtg atatggtcga gctgcaactg gaacttcgaa gtgcatcttt ttccactgag
      241 aggattgatg aactcacagc agtggtgagc ttttgcaaag acctctcttc tgattttaag
      301 ggaaatgaac tttcgttttt gtcggagagt tttataaacc ttgagcggca tttccgtgcg
      361 gctcggcgtt aagctggggg cttcgggttt agccttgttc tttttagaac cctccatttt
      421 ttggatataa gcttgagcta agtaagtgat cactttggct aaatcgtttt ctggcacgga
      481 atgggaaatc aggttctggg cttttttaag gatttcgtac tgctcttgcg tgagcgtaaa
      541 ctccattcgc acactttcat ctttttgggg tttgagtttc tgatgggttt taggggtttg
      601 attgagctca cacgctagaa ttttatctgt ttcagaacca cttttatttt caagctttcc
      661 taagagttct tgggtgcctt tgggggaaag agctttcccg gatttttgtt cttgtttaag
      721 gctttgctcc actttgacca attgggaaag atttaacgaa ccctcttcaa ttttaggggc
      781 gatgtcaggg ctgatcttca gcactcgggc ggcttgcatg cgatcatagg cggcgcttcc
      841 accatagtgc aaatgttgag tgagatactt ataaagagag tcatagccga gctctgagta
      901 aagctttctc aactcgactt cgttgatgtg gcaaaggatg aggtgggtga tttttctttc
      961 ggagcgtgac agcctttcta aacgaaaaag gatttcttgg ttggaaaccc tggaaagatc
     1021 caaggggacg ggagcttgag ctgaggactt tgcgagcttt aaagggtctt gagggggagg
     1081 gattggcata aggcactcca tttgtttaat atgttttaaa aaattctttc gtaaaaaaga
     1141 agatgagaga aggtatagca cgagtttttg taagtgaatt ttgggctttt tttttagatt
     1201 gaaggacctg aggagtgcct ctggaggagt gtttaggaat tcattagact cgttttatag
     1261 cgagtaattt tgtaagatga gaagcctctg gcagaggata aaaaaaccaa gctcttgctc
     1321 tgtgagagag cgagaaaacc ctcacaagag atatttttta aatatttatt tttacgcaag
     1381 gcttcccgga aatgccgtgt ttcaatcaag gctttgaggg aattcgagag agttcttgct
     1441 tcgttcgtct cgcctcctgg gccgaccctg cgggcaagtt tttggaaaac aaagaggaga
     1501 agagatgaag aggcaggaag ctgattctca attccaccgc ggcttttaaa gtctaaggat
     1561 aggactcttc cgattcccaa gatggtgcac aactggccca atttgaggct ttttatcgct
     1621 gactcatctt ggttcttcat caagcattcg aaagtgccga taaagagtag gatgtttgcg
     1681 aataaaagtt cggcattatt agcggttgtc ttgggtgggg ttttcctcgg cgctttctat
     1741 cagaatatga gtcctgtcga attcgccgct ctgaacatgc ctcccattga tgagggagcc
     1801 cgtcgggttc aggcaagaga gcttcttggc acttattatg gcggaagctt tgctcagagt
     1861 tttgaaggac aaaaggatat gaattactta atctatagaa agcttgaaag ttctttctcc
     1921 agtaaatgga aaaagtggct tccgcaaatg accgaagagt taattcgaga atgccaaaaa
     1981 cgggatatgg atccggtttt tattcttgcg atcattcaaa cagagagcca gtttaacgtc
     2041 tacgcaaaag gcacttccgg ggaaattggc ctgatgcaag tgttgccgca aaccgcggcg
     2101 tggatggcaa agaaatatca tctgccttgg caaggtccga actcacttta tgacccaatt
     2161 acaaatatca aattcggaat cacttatttt gcccacctgc gatcgcaatt tgaaagtcgt
     2221 gcccatcact atgtgccggc ttataatatg gggccgaaga atcttcgccg aatcgctcgc
     2281 tcgatcgtca gtatgggtga agacgaaaga gatctcctgg acggcatcta tggttcgaaa
     2341 gttatgaaga actaccaaat gatttaccaa cagatggttc aaggaaacaa aagtctaccg
     2401 aatcagtggg cacaggatgc ttcccaagaa gagttggttc gcactcgcta gcttttatta
     2461 cttagcgtca aaatttctac cagggtcaaa ttgtctctaa atattttggc ttgtcctttg
     2521 ctttagcatt tgctgaagtg caatttttat aaattgaaaa taagcaagaa atgtaaggat
     2581 agaatatgag agcaggggtt atgagtgttt tagtggcttt gtcagcggtt atggctcaag
     2641 cagcgggtcg tcaggaagcc tcggaagttt gtacaagcat gagctttgat tccgacagaa
     2701 attattgtat ttccgcaatt agtaaatatg actattttga tcaaggggcc atcaaccttt
     2761 gtcttgggat gagttttgat tctggtaaga aagagtgcat ttccaatatt ggcaataaaa
     2821 cttatgatcc ctatgaactc accaactgtg aaaaaaatga tttcgactcc agtaaaaacc
     2881 agtgtctgaa gggcgcaggg cggccgattc aaggacatgt cggttgtctg aataaccaag
     2941 atataatatt tcaactgcaa attttggagg gaatggttta tcgaggggaa aatggacgtg
     3001 ctcg
//
LOCUS       DATBTE010000351        20169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_731110, whole genome shotgun sequence.
ACCESSION   DATBTE010000351 DATBTE010000000
VERSION     DATBTE010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 20169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20169
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731110"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VIG33_13090"
     CDS             <1..735
                     /locus_tag="VIG33_13090"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEY8271819.1"
                     /translation="PSYHIQLAIESGCNAYAAPLGALEICARDFAGEIPLILKINNSE
                     TLYSNKSPISALTSHIDDALRLGCAGIGFTIYPGSAERKNMYEEIAMAAAEAKKAGLA
                     VVIWSYARGEQLSKEGETGIDVIAYSAHIAAQLGAHIIKVKPPTAHIEQEAAKKVYQA
                     QGIKVGSLADRTRHVVQAAFNGRRIVIFSGGEAKGTEEILQEVKELATGGAFGSIMGR
                     NAFQRPKKESIALLHGVMEAFVGKAL"
     gene            735..2213
                     /gene="lysS"
                     /locus_tag="VIG33_13095"
     CDS             735..2213
                     /gene="lysS"
                     /locus_tag="VIG33_13095"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEY8271820.1"
                     /translation="MSIHDNPLRAEKRRKLHALREKGINPYPYSYEKTGQAGELIERY
                     ASALNAGDKKPELVYRIAGRLMTLRSMGKASFFNVQDQTGALQVYVKPEELSEQERQA
                     FELIDLGDIVGVEGYVFKTQKGEFSLYCKKFEILTKSLEPLPEKFHGVQDVEIKYRHR
                     HLDLMTDPDSRKVFETRSKIIKEIRRFLDDRGFMEVETPTLQPIYGGAAATPFTTHHK
                     ALDMKLFMRISPELYLKRLLVGGFEKVYEISKNFRNEGIDRTHNPEFSLLEFYEAYTD
                     YNYQMDQFEELVSTLALRITGSMKVVYQEKEIDFTPPWRRLTVYDGVREYAHLDPEKA
                     SDEELFASLQKHGTREKEKFGRGEMIMELFELTAEQHLWQPTFVLDHPKEISPLTKIH
                     RKDKRLVERFEPFAACMEIGNSYSELNDPEDQLARLKEQEANRGQDDEAHPMDEDFIM
                     AIDCGMPPTGGVGLGIERVVMILVDRPSIRDIIFFPTMKITK"
     gene            2210..3118
                     /locus_tag="VIG33_13100"
     CDS             2210..3118
                     /locus_tag="VIG33_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY8271821.1"
                     /translation="MKHLILGVFFSVFFLASCAPKPTKVTSENPIIARKEAQKSKITA
                     DTVILDVRAPFEFNLSHIPSSVNVAWEDFARRAPDGRGLLQKDLHPLARRLALIGIDP
                     KTPVLVVGKGLLGKGEEGRVAWTLESLGVENVRLATVEDFRGYRSAQEIKNKNFWTPQ
                     LDVSLSIGWDELRAKIEGSAYPRTKARSKALGGVPLPAKDENFVVIDVRSPEEYSLDN
                     LNKRTPRVFRFENLEWREFFTENLDPNYRMQKILNDKGITPATEIDLISNHGVRSAAV
                     AWALQKLGYKKARNFAGGYEQVRFGK"
     gene            complement(3161..3544)
                     /locus_tag="VIG33_13105"
     CDS             complement(3161..3544)
                     /locus_tag="VIG33_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271822.1"
                     /translation="MKSLKIIFLILISSPIFALAQKPPPDDPNYRTQGHLLTLRVVPG
                     DKTVKLFFAGKKTGELDLKKDHRILSVTATGGTKQESLRFKESGEAYEVVDLPAWNEP
                     YDLSVKSRVKGKIEELRVKMPPTKP"
     gene            3576..4814
                     /locus_tag="VIG33_13110"
     CDS             3576..4814
                     /locus_tag="VIG33_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HEY8271823.1"
                     /translation="MQNSAISKSKAIIEKSLLFILATILLDTIGIGLLVPIFPKIITR
                     FATEPGAVSKYFGFFVGSYALMQFIAAPVLGALSDRFGRKRILLISLMGAALDYLFMA
                     YAPTIELLILGRVIAGLTGASMTVASSYVADISDEKSRSHNFGLIGAAWGLGFIAGPL
                     LGALLDLVGPKAPFFTAALLNLLNFFFGIFVLPESLSQEKRRALVLKRLNPLRSIIKI
                     LKPSKIAIYVWLYFLIFLAGQSLPINWNLYTEMKFHWSSLELGVSLSFMGVIVALSQG
                     LLTRYMIPRLGEERAITVGILFYAISFLLFSLSTQSWMLYVTILIFSLTGIAIPSIQS
                     LMSRNTPANEQGELQGSLVSLGSLSSVVAPLIFTPLFIHFTRTGAPFFFPGVIFSVAS
                     GISLFTLALWFYYQRSQRRE"
     gene            complement(4825..6102)
                     /locus_tag="VIG33_13115"
     CDS             complement(4825..6102)
                     /locus_tag="VIG33_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271824.1"
                     /translation="MDRQATLALQEARWRKLVRHAYEKSPYYSRIMKENRLSPETAKP
                     SDFPLLTKKIIQEHFDEIVTDPSIKQRNVHEHVEKGNSEALYLGRYHILKTSGSTGQP
                     GYFISSSEEVIAGISPSIARGHVGRRRFKKRICMVGFPKSFAGSSQVMSFCNRIWLAR
                     RLVNYRPISIEQPFENVLKELNDFQPHILSGYAKLLLLVADAQRSGKIKLSPDSIDSG
                     GEQLLETDRRYLKETFGCAVNNHYGSTEGFAMGICRDGENFIELFEDHLIININEADT
                     HITNLDGFTMPLIRYQMRDILIPHSSGIATPFQRVEPLIGRSDEIPYFITEENDRVTV
                     HPLAFDPLMPEGVKSFFMVSEKVNRVAFHIFIDEKYLNLKQQILSNVQALLLEFFSQK
                     GLPTLAIDVVYEQDYRVNTRSGKTTFWRNHVLK"
     gene            6271..6453
                     /locus_tag="VIG33_13120"
     CDS             6271..6453
                     /locus_tag="VIG33_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271825.1"
                     /translation="MENNSNFEEKISSIQIKVASKYVLVQIPCRTASFLACQDGQRPV
                     KGAVHLTELIERGGYL"
     gene            6450..8045
                     /locus_tag="VIG33_13125"
     CDS             6450..8045
                     /locus_tag="VIG33_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEY8271826.1"
                     /translation="MSDTLKGRILSTLQRFPKNVLLEDDGCSWTGEQLSSHLQSIVTR
                     IEIQSLPHSRVGVCFHNSASQAIAILAILMSDRVPVMINGVDLLESKINWIHHAKLNF
                     GIVSEDFAEKIDIPHLVINEQGLPVELIGKTNVSAEVDQVLHMPPAGTGLVLFTSGST
                     GEMKEVYIPALGILKTIDELIKKFNLSEKTVATVTLPVCHSMALNTQFLPTFMVGGKC
                     HFCNLRLSMNKVYRGLLRDNSTFVALIGEIVRTCWEERRLKKLPPCDQVEHVQIAGGL
                     ISLKHLEMTRELFPKAIIHKGYGLTEAIRVTMISSQEEKFNSTAVGKPLSFLELQIRN
                     EHGEILPAGQYGEIHVKGDSVMLGLRSLTSANSRLVAREEFLATGDLGMMDEEGYLHV
                     TGRLDSLFKINGRRVFGVEIEKVALEACDKFRFAKAILVSDEDRDRSRIVLFIELDRS
                     CWENNILRLETEVQDRLAQRMKMLSIPPKEIIFARHIPRTSNGKLNLPMLKQIWKIQS
                     VRPIEKTIAANLNLFSIDEEITA"
     gene            8042..9283
                     /locus_tag="VIG33_13130"
     CDS             8042..9283
                     /locus_tag="VIG33_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271827.1"
                     /translation="MRLLGLIFCIGLAFHCDIGAAKPFKKVLVISGGGVNPGLALGII
                     AGVQEKGWRPDLIIATCGAGIGSAIYNSEQSIAGSFEVLRSWEFYKTLSRIEIETPNG
                     LDMFDKLKKAKNTFVYPSLFAGNLLHAPEEFFLQLKNPEFNQNPALPKVIILSARAFF
                     GPSQVGQVRPFAPIFQQVYFTDAGTAEYLKGRKLPKKYSYPFTTLTEDTLAISEHDTI
                     TASRAGGADPYLLNPSLVDGYYHFSGAVDLFPLDLAVSLGDEVVATYPASLFQDYEDV
                     AVVSGFGFKQTTRALEAIQHKDVKWIDISGADDLSFNPSRLILLMRSGIPTDFTEFQL
                     GVGKQWSFGRDRANEAIRMASGKVTDIRFHLRKPINPKLAEDFSCKNANEWKTDQRDS
                     CVNDKSAECNRALAKKCVPIR"
     gene            9301..9765
                     /locus_tag="VIG33_13135"
     CDS             9301..9765
                     /locus_tag="VIG33_13135"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HEY8271828.1"
                     /translation="MRWLLLDQILECEPGVKAKGVKTFSRAEVFFMDHFPGMPIVPGV
                     LQIEMMAQLAGKCYAIANPEVLPVLGTVKAAKFYGNIQPGDRCVIHAAIVKAAKGFLV
                     AEASVEVEGKKVSSATILFGQVPRSRLSSQSFDAVVEDWKKKQQQEEIHGTK"
     gene            9752..10549
                     /locus_tag="VIG33_13140"
     CDS             9752..10549
                     /locus_tag="VIG33_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271829.1"
                     /translation="MGLNNPLNPIKLLWRKMWIPDLTTEPQTWEDKTFQDQIPNVRRV
                     ETENSDILVPDPKAVRSMSRASLFMSSMAEEMKPFLAPYLEKSPFSVGIYCAVENGPI
                     DGPSTAEILNRSTEAFDFKFAEAYRKLRNPKMYLKQLPNLAPAQLGISLGIQGPMNVY
                     THSLYACQQALEQAEADLLNKKVEAALVLTAHAFDDFWVVKRSRHQEKRTLCEGAATL
                     ILSLSEGSVSESAPEVMKKSANREYYFDAKNYFGISDPLINLLRKWD"
     gene            10554..10832
                     /locus_tag="VIG33_13145"
     CDS             10554..10832
                     /locus_tag="VIG33_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY8271830.1"
                     /translation="MSQTLLNSIKDAIKDVVNNQKLNIEPTSKLIDDLGLESIDFLDL
                     SSEIENTIGFELDFKEVVEYLKQTAGGATDIKAVTVQNLIDYIQTKQA"
     gene            10843..12126
                     /locus_tag="VIG33_13150"
     CDS             10843..12126
                     /locus_tag="VIG33_13150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEY8271831.1"
                     /translation="MKKADVVITAYGLVSPLGSTIEDFESGLFSGKSGVKSIRGSTPD
                     SMVAEDFPVPYAATIDRKTLPQTKYFGPQEAPPLMKSWLMTAVATESILAKLDPQHPV
                     DAIVYGTADGVSFEQVAEVLKHKKQQDFDEADYESQYDFMRTRCESSVEVIHNILKDS
                     GFAEIPGERRICINSACATGNNALGIAFEAIQAGRWERCIVGGVDARCEPSNFLNFYY
                     LGALTTDNVAPEKASRPFSVDRSGFVRGEGAAVLMVESYESAVARGAQILCRVVGYGF
                     TSDAFRLTDGRDDGSSVMKAMELAIQSAGLTPHDIDYINAHGTSTPLNDRLESLAIKK
                     VFGEKAYKTPISSLKSQIGHSTIASSALEAIACALMLQNQKVAPTLNFHGGDPDCDLD
                     YVPEGSREIPLTHVLSNSFGFGGQNSCIVFAKGNK"
     gene            12123..12890
                     /locus_tag="VIG33_13155"
     CDS             12123..12890
                     /locus_tag="VIG33_13155"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8271832.1"
                     /translation="MSRRLAVITGGTSGIGLGIAQKLIQNFDLALVYGNNHERAEQAK
                     NLLEQTSSGAKVQSYSKDLLQLEEIPSLIKKITEDFAIAPSVLVNSAGRLRDGLFQSS
                     KMQDHEDLIREHLLAPMALCHAVVKGMSVGKFGRIIHLSSISADFAKRGQTNYAAAKG
                     GIESFTRTLALEVAHRGITVNAIAPGLIETPMTQQLVEKIQSSNEGIRSRIPVGRLGT
                     PADIGAVVAFLCSDDASYITGTTITVDGGRSLGDPQS"
     gene            12887..13639
                     /locus_tag="VIG33_13160"
     CDS             12887..13639
                     /locus_tag="VIG33_13160"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8271833.1"
                     /translation="MSQFKQKTAVITGGTRGIGRAVSLKLARQGAKVLALYGRDSKSA
                     AELESLAQQEGLEILCIKGDLCNEEKFADVVAKIKTNTAQVDFLVHCAASGVHKNAME
                     LSLKHLRWTFEINVYAIHHLLQNLIELMPRGSRVIGITSSGGTRVIPFYAAIGSSKGA
                     LESLFRHYAFELAPKGIAVNLVCPGLVLTDAVEAFPDREARINNTKRVTPTGELVVPE
                     DVAETVSFLCEPRAAQIIGQTLVIDGGKTLLS"
     gene            13662..14483
                     /locus_tag="VIG33_13165"
     CDS             13662..14483
                     /locus_tag="VIG33_13165"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY8271834.1"
                     /translation="MLHSLGNEKAPILVCVPGLLGGAEDFRGIIDPLLSEFHVLIFDP
                     NASRRQFGLNGLTVEVMQEIQFDCTADEIAIELAKFTSEPILMCGISLGGKIVYDFAH
                     KFPHLFKGAVITDVSPGSFGESRLFQFVEGLVSDLNLNQDWPGLKKELRERIPEPSLR
                     SLIQSQLFYPTKQPPAAWKVGMKHFRALLQRQAMDEQFKALMKVDELLLKQGSEIRVL
                     KAENLSAIDDISFGEMKKMKSIKIYPLMGASHFIHITHKQDIQNLLMQLKTSVSV"
     gene            14636..14905
                     /locus_tag="VIG33_13170"
     CDS             14636..14905
                     /locus_tag="VIG33_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271835.1"
                     /translation="MKRNRVLVAAVVFLSSMSFAEAGGSRQDDSLDQVPSMSGKIEIM
                     EELGLIEHPSPFQQISPRKTSNSKKAVRQKVRMGHLADISVDGEH"
     gene            complement(15529..15873)
                     /locus_tag="VIG33_13175"
     CDS             complement(15529..15873)
                     /locus_tag="VIG33_13175"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEY8271836.1"
                     /translation="MARFKQFEVIVVPFPYSDSPKSKNRPALVLSDSDNFDGKTGHSV
                     CAMITSAKNKSWPLDIEVTDLKSCGLPAPSVVRMKLFTIDHQIIKEKIGELAHKDKAL
                     VTKSLKAVIPLL"
     gene            complement(15860..16084)
                     /locus_tag="VIG33_13180"
     CDS             complement(15860..16084)
                     /locus_tag="VIG33_13180"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY8271837.1"
                     /translation="MKASKLTSKYQATVPKEVRAFLGLASGDGLQWEIEDGHVVVKKI
                     SKIDLEWQKSIEMTLSEWNSPEDDEAYGSL"
     gene            16278..17069
                     /locus_tag="VIG33_13185"
     CDS             16278..17069
                     /locus_tag="VIG33_13185"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY8271838.1"
                     /translation="MEKARGLLIFFLLFTVTSWAEESSFELALPELESSSPSSSNFVS
                     SFEKEPGNNFSNEPYESSTSAQTSAEPVKGVYENAFTSYQSKKTAEEENTATDEWRKG
                     LDKQDNFDLENLTFSEPKTAPHPRGQARRPTVASATDVAEDKIPMPGLDNNQESSAPP
                     VSAKDRKAAVMKTISDNYEELKVCYQEGLKKNSEMKGKVVLGWSMDTQGRVSGVEVLT
                     SQLNNKQVEKCMAEHLANWHFPRQAKLRSSKDRMTYAFQFVSEKE"
     gene            17287..17529
                     /locus_tag="VIG33_13190"
     CDS             17287..17529
                     /locus_tag="VIG33_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271839.1"
                     /translation="MNKLALSLIFTFGTTVAYAGSSNSDKDIWDALNVEVEQLPTLPG
                     YSIGEAKRVGRLSCSHIKQDGYAEVFTCSLKEFAGH"
     gene            complement(17526..19490)
                     /locus_tag="VIG33_13195"
     CDS             complement(17526..19490)
                     /locus_tag="VIG33_13195"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEY8271840.1"
                     /translation="MKTVVASISLLLTLTSSASFAGIHHALEVTLSPAVKSLMILDQL
                     SLSASKRYHFLLNKNLQVKITSPGDQLRLLPSTKPAANYNEYELQTDAADHFVHLQYS
                     GVIFEPITDNSTSGLISPEGAILFGAAYWLPDFSEKATYEIIKSDLPKNWKIVSPLNQ
                     LSLPQQDIYLVAGDFQEYALDNNPSGIPLKVYLRHPEPQLAQTFLSLLPDYLDHYQKT
                     LGTFPYQSFAVIENFWETGFGMPAFTLLGPNVIRLPYILNSSLPHELLHNWWGNSVYV
                     DYNRGNWCEGLTTYLADHWQQETLRADRDYRRQSLMNFQDYAKSANDFPLRQFKERFN
                     FGSQAVGYGKGMMFFHMLKKQLGTPLFEQGLRRLYWNHRGQSISYEEIETTFEAISHL
                     DLKSFFNQWLDRTGVPKLSLHQVQLSKLGAQKFKVNFEIHQTSPQIYVLEVPVRLSFA
                     DGKVQNELVRVKSKIAKFQFVLRDTPKSLEIDPDFDVFRELDLKEQSLSFSNVFGSDH
                     LWIVGTNETEKLSYQKSWQAALGIPITLSEDKLLLDLPKKGTILLLGDSPQYEKLMAK
                     ELHGQDFKLNPEEIILFGTSYLRREYKSALLARSLNHPELIFAWIRGPGLETLAPRLL
                     HYGKYGVLIFSEKAVPLKTTWPILASPLKF"
     gene            19698..>20169
                     /locus_tag="VIG33_13200"
     CDS             19698..>20169
                     /locus_tag="VIG33_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271841.1"
                     /translation="MRIAVVLVSLCFLLASCATTLSGEEGTPSEVSPQTEVSPPVPLP
                     TEVTHETHESQEPVDLGSPNLESQAAAPETSASGLDTFYASEQQSPGVDQVAPETQVP
                     PTILPEKIHLKRKYFNVDSSYEDQKPEKPAYKKSKNLAKKRAKVSKRDKNKIAKN"
BASE COUNT         5379 a         4169 c         4838 g         5783 t
ORIGIN      
        1 ccttcttatc atattcagct tgcgattgag tcaggctgca atgcttacgc agcacccttg
       61 ggagccctgg aaatctgtgc ccgtgacttt gcgggcgaaa ttcccctgat tcttaaaatc
      121 aataattccg aaactcttta ctccaataaa tcccctattt ccgcactcac ttcgcacatt
      181 gatgatgccc ttcgtttggg gtgtgcaggg attggtttca cgatctatcc gggtagtgct
      241 gaaagaaaaa atatgtatga agagatcgca atggctgctg cagaagcaaa aaaagcaggc
      301 ctggctgtgg tgatctggtc ctatgctcgc ggcgagcagc tctctaaaga gggtgaaacc
      361 ggaattgatg taattgccta ttcagctcat atcgccgctc aactaggcgc ccacattatc
      421 aaagtaaaac caccaacggc gcatattgag caagaggctg cgaaaaaagt ttatcaggcc
      481 caaggcatta aagtggggtc tttagcggat cgcactcgcc atgtggttca agccgctttc
      541 aacggccgcc gtatcgtcat cttctctggt ggcgaagcca aaggcaccga ggaaattctg
      601 caagaagtaa aagagcttgc tacgggtggc gcatttggaa gcatcatggg acgcaacgcc
      661 ttccaaagac ctaagaaaga atcgattgcc ctcctccatg gagtgatgga agccttcgtc
      721 ggaaaggcgc tttaatgtcg attcatgaca atccattaag agcggagaaa cgccgtaaac
      781 tgcacgctct tcgtgaaaaa ggaatcaatc cctatcctta ctcctatgag aaaacgggtc
      841 aggcggggga acttattgaa agatacgcaa gtgctttaaa tgccggcgat aagaagcctg
      901 agttggttta tcgtattgcg ggtcgtttaa tgactttgcg ttcaatgggg aaggcttctt
      961 ttttcaatgt tcaagatcaa acgggtgctt tgcaggttta cgtaaagccg gaagaacttt
     1021 ctgagcagga acgccaggct tttgaattga tcgacttggg tgatatcgtt ggggttgaag
     1081 gctatgtttt caaaacccaa aaaggcgagt tttcactgta ctgcaagaaa ttcgaaattc
     1141 tgaccaagtc cctggaacct ttgccagaaa agtttcacgg agtgcaagat gtggaaatta
     1201 aatatcgcca tcgtcatttg gatttgatga ccgatccgga ttcccgcaag gtttttgaga
     1261 ctcgttcaaa aatcatcaaa gagattcgtc gctttttaga tgaccgtggg tttatggaag
     1321 tggagacgcc aacgcttcag ccgatttacg gcggggctgc cgcgactcca tttaccactc
     1381 atcataaagc attggatatg aagcttttca tgagaatttc tccagagctt tatttgaaac
     1441 gtctgctcgt gggtggcttt gaaaaggtct acgagatcag caagaacttc cgtaatgagg
     1501 ggattgaccg cacacacaat cctgagttct cgttgcttga gttttatgag gcctacacgg
     1561 attacaatta tcaaatggat caattcgaag aattggtttc aactttggct ttgcgaatca
     1621 ccggcagcat gaaagtggtt tatcaagaaa aagaaatcga tttcactccg ccttggagac
     1681 gtttgacggt ttatgatgga gttcgtgagt acgctcactt ggatcctgaa aaagccagcg
     1741 atgaggagct ctttgcttct ttacaaaagc atggaacccg tgaaaaagaa aagttcggac
     1801 gtggtgaaat gatcatggaa cttttcgaac tcacggcgga acagcatttg tggcaaccca
     1861 cttttgtcct ggatcatccg aaggagattt ctcccttgac caagatccat cgcaaagaca
     1921 agcgtttggt ggagcgcttt gagccttttg ccgcttgtat ggaaatcggc aattcttatt
     1981 ctgaattgaa cgatccggaa gatcagttag cgcgtttgaa agaacaagaa gcgaatcgtg
     2041 gccaagatga cgaagctcat cccatggacg aagacttcat catggcgatt gattgcggca
     2101 tgcctccgac gggcggggtc gggctcggaa ttgagcgagt ggtgatgatc ctcgtggatc
     2161 gcccaagtat tcgcgatatt attttcttcc caaccatgaa gataacaaaa tgaagcattt
     2221 aattcttggt gtttttttca gcgttttttt cttagcatct tgcgctccga agcccaccaa
     2281 ggtgacttcg gaaaacccca tcatcgctcg gaaagaagcg caaaagtcca agatcaccgc
     2341 tgacaccgtg atcttggacg tgcgggcgcc ttttgagttt aatttgtctc atatcccaag
     2401 ctccgtgaac gttgcgtggg aagattttgc gcgccgtgcg ccggacggtc gcggtttgtt
     2461 gcaaaaagac cttcatcccc tcgcacggcg tttggctttg attgggattg atcctaaaac
     2521 tcctgtcctg gttgtaggaa aaggattatt aggcaaagga gaagaggggc gtgtggcctg
     2581 gactttggaa agtctggggg tggaaaatgt ccgcttggcc accgtagaag atttccgcgg
     2641 ctatcggagt gcacaagaaa tcaaaaataa aaatttctgg acaccgcaat tggatgtgtc
     2701 cttatctatt ggttgggacg agcttcgcgc caagatcgaa ggttctgctt accccagaac
     2761 caaagcccgc agcaaggctc tgggcggagt cccgcttccg gcaaaagacg agaactttgt
     2821 agtgatcgat gtgcgttccc cagaggagta ttccctcgat aatctcaaca aaaggacgcc
     2881 gcgagttttc cgttttgaaa atttggagtg gcgcgaattt tttactgaaa atctggaccc
     2941 aaattatcgc atgcaaaaga ttctcaatga caaaggaatc actccggcaa cggagattga
     3001 tcttatcagt aatcacggcg ttcgctccgc agcggtcgct tgggctctgc aaaagttagg
     3061 ttataaaaag gcccgcaatt ttgctggcgg ctatgaacaa gtccgttttg gcaagtaagg
     3121 aagcagatcg gtgaaggagc aaaagcctcc ccggggccca ttagggtttt gtaggaggca
     3181 tttttactct gagctcttcg atttttccct tcacccggga cttgacagaa agatcatagg
     3241 gctcattcca agcaggtaaa tccaccactt cgtaagcctc cccactttct ttaaatctca
     3301 atgattcttg ctttgtcccg cctgtggcag tcacggataa gatcctatga tcttttttca
     3361 agtccagttc tccagttttt ttgcctgcaa aaaaaagttt taccgtttta tcgcctggca
     3421 ccactcggag agtaaggaga tggccttggg tcctatagtt aggatcgtcg ggtggtggtt
     3481 tctgtgcaag agcgaaaatg ggcgacgaaa taagaattaa aaaaataatc ttcagtgatt
     3541 tcataaaagt cctttttaaa gtatcatatg ttggcgtgca aaactcagca atcagtaaat
     3601 ctaaagcgat tatcgaaaaa agcttattat ttattttagc aacgatattg ctggatacaa
     3661 tagggatagg gcttctggtt cctattttcc ctaaaatcat cactagattt gccaccgaac
     3721 caggagctgt ttccaaatat tttggctttt ttgtgggctc gtatgcctta atgcaattca
     3781 ttgcggcgcc agtgctgggg gctctttcgg atcgttttgg ccgtaagagg attcttctga
     3841 tatctcttat gggagccgcc ctagactatt tatttatggc ctatgccccg accatcgagt
     3901 tgcttattct aggacgagtg attgcgggat tgaccggagc gagcatgacc gtggcctctt
     3961 cctacgtagc ggatatcagc gatgagaaaa gtcggtctca taattttgga ctgatcggcg
     4021 cggcctgggg actgggattc attgcgggtc ctctgcttgg ggccttgctt gatttagtag
     4081 gtcccaaggc tccttttttc actgcggcct tgctgaatct cctcaatttt ttcttcggaa
     4141 tattcgtatt gcccgaatcc ttgagtcagg aaaagcgcag agccttggtt ctcaagcggc
     4201 tcaatccatt gcgttcaatt ataaagattc ttaagccttc gaagatcgct atttatgttt
     4261 ggctttattt tttaattttc ttagcgggtc agtctcttcc catcaattgg aatctttata
     4321 cggaaatgaa atttcattgg tcttcgttag agttgggagt ttctctctct tttatgggag
     4381 ttattgtcgc tttatctcag ggcttactca cgcgctatat gattccacgc ttgggcgaag
     4441 aaagggccat caccgtcggg atcctgttct acgcaattag ctttttgtta tttagcctct
     4501 ccacgcagtc gtggatgctt tatgtcacca ttttaatttt ttctctgacg ggaatagcaa
     4561 ttccctcaat tcaatccttg atgtcgagaa atactccggc caatgagcaa ggggagttgc
     4621 aagggagttt ggtctcttta gggagtctct cctccgtagt ggcaccgctg attttcacac
     4681 ccctatttat tcatttcacc agaacgggtg ctcccttttt ctttccagga gtcatatttt
     4741 ccgtggcttc tggaatttcg ctcttcaccc tggccctctg gttttattat caaagatccc
     4801 aaagaagaga ataagcttga ggttttattt tagtacgtga ttccgccaaa aagtcgtttt
     4861 ccctgacctc gtattcaccc gataatcttg ctcatacacg acatcaatag ctaaagtggg
     4921 cagtcctttt tgactaaaaa actcaaggag cagagcctga acattgctca gaatttgctg
     4981 tttgagattt aaatatttct catcaataaa aatatgaaat gcgacgcgat tgactttttc
     5041 actcaccata aagaaggatt tcacgccctc aggcatgagg gggtcgaagg ccaagggatg
     5101 gacggtgact cggtcgtttt cttcagtgat aaagtaggga atctcatcag aacgtccaat
     5161 gaggggctcg actcgctgaa agggcgtagc tatgccggaa ctgtgaggaa ttaaaatgtc
     5221 ccgcatttga tagcggatga ggggcatggt aaaaccatct aaattggtga tgtgagtatc
     5281 tgcttcattg atgtttatga tcaaatgatc ttcaaacaat tcaataaagt tttctccatc
     5341 ccggcaaatg cccatggcaa agccttcggt gcttccatag tgattattga cggcgcagcc
     5401 aaaggtttct tttagatagc gacgatctgt ctctaacaat tgctcccccc cagaatctat
     5461 ggagtcggga ctgagtttta tttttccact tctttgggca tcggccacca atagcaaaag
     5521 cttcgcataa ccactcagaa tatggggttg gaaatcatta agttctttta gaacattttc
     5581 aaaaggttgt tcgatggaaa tcggtcgata gttcacaagt cgtctagcta gccagatacg
     5641 attgcaaaag ctcatgacct gggaagagcc tgcaaagctt ttggggaagc cgaccataca
     5701 gattcgtttt ttaaaacgtc gcctccctac atggccgcga gctatagagg gagaaattcc
     5761 agcgatcact tcttcagaag aagaaataaa atatccaggc tgccccgtgc ttccggaagt
     5821 ttttaaaata tgatagcggc caagatagag ggcttcagaa ttgccttttt caacatgttc
     5881 atgaacattt ctttgtttga ttgaggggtc cgtgacaatt tcatcgaagt gctcttgaat
     5941 gatctttttg gttaaaaggg gaaaatcact cggttttgca gtctcaggtg aaagcctgtt
     6001 ctccttcata atacgagaat aataaggtga tttttcataa gcatggcgta ccaattttcg
     6061 ccagcgggct tcttgaaggg ccaacgttgc ttgccggtcc atttttgaca ccttaatgca
     6121 atctagcacg tgctttacgt atttgtagga aagcatataa cccccgaggc tatattagtt
     6181 taggcttttt ttctttagtt atcaatggat tgagagtttt actcttcaaa cattatttgc
     6241 tagtaattgt ttgtttgaag ggagaggagc atggagaata attcaaactt tgaagaaaag
     6301 atttcatcaa ttcagattaa agttgcttca aagtatgtat tggttcaaat tccttgccgc
     6361 accgcttctt ttttagcctg ccaggatggc cagaggcccg taaaaggggc agtccatttg
     6421 accgagttga ttgaacgagg gggctattta tgagtgatac cttgaaaggt cgcattctaa
     6481 gcacattgca acgatttcca aagaacgtgc ttttggaaga cgatggctgc tcctggacag
     6541 gagagcaact gagttctcat cttcaatcta ttgttaccag aatcgagatc caatccttgc
     6601 ctcactctcg agtgggagtt tgtttccata atagtgcttc ccaagccatt gctattttag
     6661 cgattctcat gtccgatcga gtaccggtta tgatcaacgg tgtggatttg ttggaaagta
     6721 agataaattg gattcaccac gcaaaactta atttcggtat tgtatccgaa gacttcgctg
     6781 aaaaaataga tattccccat ttagtcataa acgaacaggg gcttccagtc gaattgattg
     6841 gaaaaaccaa tgtttccgcg gaagttgatc aagtactgca tatgcctcca gcggggacgg
     6901 gtctggtgtt atttacctca ggctctaccg gtgaaatgaa agaagtttat attcccgctc
     6961 tgggaattct gaaaacgatt gatgaactca ttaagaaatt caatttaagc gaaaaaaccg
     7021 tggccacagt aactcttcct gtgtgtcaca gtatggcttt gaatactcag tttttaccga
     7081 ccttcatggt ggggggcaag tgtcacttct gtaatttacg tctttcgatg aataaggttt
     7141 accgtgggct cttgcgcgac aattccactt ttgttgcgct gatcggagag attgttcgca
     7201 cttgttggga agagcgtcgc cttaaaaaat tgcctccttg tgatcaggtg gagcatgtgc
     7261 aaattgcagg gggattgatt tcgcttaagc acttagagat gactcgtgag ctttttccta
     7321 aggccattat tcacaaaggt tatgggctga cggaggcgat ccgggttacc atgatatcta
     7381 gtcaagagga gaagtttaat tccaccgcgg ttggcaaacc tttgtctttt cttgaattgc
     7441 agattcgtaa tgaacacgga gaaatccttc ctgccggtca atatggcgag attcatgtca
     7501 aaggtgacag cgttatgctt ggcttacggt ctttgacttc agccaattcc cgtttggtgg
     7561 cacgagaaga atttttagct actggcgacc ttggcatgat ggatgaagaa ggctatctgc
     7621 acgtgacagg gcgtctggat agtttattta agattaatgg acggagagtc tttggagtgg
     7681 aaatagaaaa ggtggctctc gaagcttgcg ataagtttcg ttttgcgaaa gccatcttgg
     7741 tctccgatga ggaccgggat cgttcacgca tcgtcctgtt cattgaactc gatcgttctt
     7801 gttgggaaaa taatatcctt cgtttagaga ccgaggttca agaccgcttg gctcaacgta
     7861 tgaaaatgtt gagtattcct ccaaaagaaa tcatatttgc gcgacacatt cctcgcactt
     7921 caaacggtaa attgaatctt cctatgttaa aacaaatttg gaaaattcaa tcggttcgtc
     7981 cgattgagaa aacaatagct gctaatttaa atttattcag cattgatgag gagataacag
     8041 catgagactt cttggtttaa ttttctgtat aggcttggca ttccattgtg acatcggcgc
     8101 agcgaaacct tttaaaaaag ttttagtgat cagcggcggc ggggtcaacc cgggattggc
     8161 cctggggatc atcgctggag tccaggaaaa gggctggcgg cctgatttga ttattgcgac
     8221 ttgtggtgcg ggcattggta gtgctatata caactcagaa caatcgattg ccggaagctt
     8281 tgaagtcctt agatcttggg aattctataa aacattgagc cgaattgaga ttgaaactcc
     8341 caacggcctt gatatgttcg ataaattaaa gaaagctaaa aacacctttg tctatccctc
     8401 tctttttgcc ggaaatttac tgcatgctcc ggaagaattt tttcttcagt tgaaaaatcc
     8461 tgaattcaat cagaatcctg ctttgccaaa ggtgattatt ctttcggcgc gagcgttttt
     8521 tgggccttcc caggttggtc aagtcagacc ttttgcccct atttttcagc aggtctattt
     8581 taccgatgca ggcacggctg agtatctcaa gggaaggaag cttcctaaga agtattctta
     8641 tccgttcacc accctgacag aggatacttt agcgatcagc gaacacgata ctatcacagc
     8701 ttcacgtgcg gggggcgccg acccttattt actgaatcca agtctggtgg atggctacta
     8761 tcacttctct ggcgcggtgg atttatttcc actggatctt gccgtgagtc ttggtgacga
     8821 ggttgtagcc acatatccag caagtttatt tcaagattat gaagatgtcg cggtcgtttc
     8881 aggatttggt tttaagcaga cgacacgagc tttggaagcc attcaacata aagatgtaaa
     8941 atggattgat ataagtggcg ctgatgacct gagtttcaat ccttcgcgtt tgattttgtt
     9001 gatgcgcagt ggaattccca cggattttac tgagtttcag ttaggcgttg ggaaacagtg
     9061 gagttttggc cgcgatcgag cgaatgaggc catccgtatg gcttccggca aagttactga
     9121 cattcgtttt cacctgcgta aaccgatcaa cccgaaatta gcagaagatt tttcatgtaa
     9181 aaatgcaaat gaatggaaaa ccgatcagcg cgattcttgc gtcaatgaca agtccgcgga
     9241 atgcaatcga gccttagcga aaaagtgcgt accaatccgt taatttagac agggaaaacc
     9301 atgcgttggc tactattaga ccaaatttta gaatgcgaac ccggcgttaa agccaagggc
     9361 gtaaagacct tttctcgtgc tgaggtcttt ttcatggatc attttccagg aatgcctatt
     9421 gttcctggag tattgcagat tgaaatgatg gcgcaattgg caggcaagtg ctacgccatt
     9481 gccaatcctg aagtcttgcc ggttttgggg acagtgaaag ccgctaaatt ctatggaaac
     9541 attcagccag gagatcgttg cgttattcac gccgcgatcg tgaaagcggc caaaggtttt
     9601 ttggtggccg aagcttcggt tgaagttgaa ggcaaaaaag tttccagcgc cacaatttta
     9661 tttggccagg tgccccgcag tcgattgagt tcacaaagtt ttgatgcagt ggttgaagac
     9721 tggaaaaaga agcagcagca ggaggaaatc catgggacta aataatccct tgaaccctat
     9781 caaattgctt tggcggaaaa tgtggattcc tgatctcaca acggagcctc aaacttggga
     9841 agacaaaacc tttcaagatc aaatccccaa tgtccgccgg gtggaaactg aaaacagtga
     9901 tattcttgtg ccggatccaa aagcggtgcg ctccatgagt cgtgcgtctt tattcatgtc
     9961 gagtatggct gaggagatga agcccttcct agctccgtat ttggaaaagt cccccttctc
    10021 agtagggatc tattgtgcgg ttgaaaatgg cccgattgat gggccctcaa cagcggagat
    10081 attaaatcgc agcactgagg cttttgattt taaattcgct gaggcctatc gtaaactgag
    10141 aaatcctaag atgtatttaa agcagcttcc taatttggcc ccagctcaac tgggaatttc
    10201 cctgggcatc caaggaccga tgaacgttta cacccattca ctctatgcct gccagcaagc
    10261 cttggaacaa gcagaagcag atcttctcaa taagaaggtg gaagcagctt tggttttaac
    10321 ggcccatgcc ttcgatgatt tctgggttgt aaaacgatct cggcatcaag agaaaaggac
    10381 tctctgtgaa ggagctgcaa ctctaattct gagtctctca gagggatctg tttcagagag
    10441 tgctccagaa gtgatgaaaa aaagtgcaaa cagagaatat tactttgatg cgaagaatta
    10501 ttttggtatt tctgatccat taattaactt gcttagaaag tgggattaaa aaaatgtcac
    10561 agactcttct aaattcaatt aaagatgcca tcaaagatgt tgttaataac caaaagctca
    10621 atatcgaacc tacaagtaaa ctgattgatg atcttggtct tgagagtatt gattttctcg
    10681 acctcagctc agaaattgaa aataccattg gttttgaatt agatttcaaa gaagtggttg
    10741 agtatcttaa gcagacggct ggcggtgcta ccgatattaa ggctgtgact gtgcaaaacc
    10801 tgattgatta tattcaaaca aaacaagctt aggacttcat ttatgaagaa agcggatgtt
    10861 gtcataacgg catatggttt ggtctctccc ctgggtagca ctattgagga cttcgaaagt
    10921 ggtctgttta gtggcaagag cggagtgaaa tccattcggg gatccactcc agactccatg
    10981 gtagcggagg attttcctgt tccttatgcc gccactatcg atcgcaagac actcccgcaa
    11041 actaagtatt ttggcccgca agaggcccct ccactcatga aatcatggct gatgacggca
    11101 gtagcgacag aatctattct tgccaagctg gatccacaac accccgtcga tgccattgtt
    11161 tatgggacgg ccgatggtgt ttccttcgag caagtggctg aagttcttaa gcacaagaaa
    11221 cagcaagact tcgatgaagc tgactatgaa tctcaatatg atttcatgcg aactcgttgt
    11281 gaatcttccg tagaagtgat tcacaatatt ctcaaagact cgggttttgc ggagattcca
    11341 ggggagcgtc gcatttgcat caactccgcc tgcgccaccg gcaataatgc tttaggaatt
    11401 gcttttgaag cgattcaggc tgggcgctgg gagcgatgta ttgtaggcgg tgtcgacgcc
    11461 cgttgtgagc ccagcaattt tttaaatttt tactatctag gagctttgac cacggacaat
    11521 gttgcgcctg aaaaggccag tcgtcctttt tcagtggatc gttcgggttt tgtcagaggt
    11581 gaaggcgctg ccgtattgat ggtggaaagt tatgaatcag cggtggctcg tggggcgcag
    11641 attctttgcc gggtggtggg atatggattt accagtgatg ccttccgctt aacggatggt
    11701 cgtgatgatg gttcttcagt gatgaaagcc atggaattag ccattcaatc ggcaggtctt
    11761 actcctcacg atatcgatta cataaacgcc catggaactt ccactccgct gaatgatcgt
    11821 ttagagtctt tggccattaa aaaagttttc ggagagaaag cctataaaac tccgatcagc
    11881 tctcttaaat ctcagattgg ccattctacc attgcctcta gtgctttgga ggctattgcc
    11941 tgcgctttga tgctgcaaaa tcaaaaagtc gcaccgacct tgaactttca tggtggagat
    12001 ccagattgtg atctcgatta tgttcctgag ggctcacgag aaataccttt aactcatgta
    12061 ctgagtaata gctttggttt cggcgggcaa aattcttgta tcgtgttcgc caagggcaac
    12121 aaatgagccg tcgactcgca gtcatcacgg gcggaacttc tgggattggc ttagggatag
    12181 ctcaaaaatt aattcagaat tttgatttgg ctctggttta tggaaataat cacgaacgag
    12241 ctgagcaagc taaaaacctt ctagagcaaa cttcttcagg ggcgaaggtt caaagctatt
    12301 ctaaagatct gcttcagttg gaggaaatcc cttctttgat taaaaaaatc acggaagatt
    12361 tcgccattgc tccctcggtg ttggtgaatt ccgcaggtcg cttgcgcgat ggactcttcc
    12421 agagttccaa aatgcaggat cacgaagatt tgattcgtga gcatctctta gctcccatgg
    12481 ctctttgtca cgcagtcgta aagggaatgt ccgtggggaa attcggtcgg atcattcatt
    12541 taagctctat cagtgctgat ttcgccaaac gtgggcaaac caactatgct gcggctaagg
    12601 gggggattga gtcttttacc cgcaccttgg cattagaggt ggcccatcgc gggatcacag
    12661 ttaatgccat cgctcctggg ctgattgaaa cgcccatgac tcaacagttg gttgaaaaaa
    12721 ttcagtcttc caatgaaggg attcgcagtc gaattcccgt tgggcggttg ggcacccctg
    12781 cggatattgg tgcagtcgtg gcgtttttat gttcggatga cgcttcttat attaccggaa
    12841 cgacaattac cgtcgacgga ggacgatctt taggagatcc tcaatcatga gtcaattcaa
    12901 acagaaaacc gcagtgatca cgggtggaac tcgaggaatt gggagagctg tcagcttaaa
    12961 gttggctcgt caaggagcca aggtattggc gctttatggc cgcgattcta agagtgccgc
    13021 tgagcttgaa tctcttgctc aacaagaggg actcgagatt ctttgtatca agggtgatct
    13081 ttgcaacgag gaaaaatttg cggatgtggt ggcgaaaatc aaaaccaata ctgcgcaagt
    13141 tgatttcctg gttcattgtg cggccagcgg tgtgcacaaa aatgcgatgg aactttctct
    13201 caagcatttg cgctggactt ttgaaatcaa tgtgtacgct attcatcatt tgttgcaaaa
    13261 cctgattgaa ctgatgccac ggggttcccg agtgattgga attacctcct ccgggggcac
    13321 tcgagtgatt cctttctatg ccgctattgg cagtagtaaa ggggccttgg aatctttgtt
    13381 tcggcattat gcttttgaac tagcacctaa aggcattgct gtgaatctcg tttgtcctgg
    13441 gttggtttta actgatgctg tagaagcctt ccctgatcga gaggctcgca ttaataatac
    13501 caaaagagtg acccctacag gggaactcgt tgtccctgaa gatgtcgctg agactgtttc
    13561 attcttgtgt gagccgcgag cggcacaaat cattggacaa acgcttgtca ttgacggtgg
    13621 taaaactctg ctcagctaat actgcaaagg gagaaatcga tatgctacat agccttggaa
    13681 atgaaaaagc gcccattctt gtgtgtgttc cgggtctttt gggaggcgca gaagattttc
    13741 gcggtattat tgatcctttg ctgagtgaat tccatgtttt gatttttgat cccaatgctt
    13801 cacgtagaca attcggttta aatgggctta cggtggaagt gatgcaagag attcagtttg
    13861 attgcactgc cgatgaaatt gccattgaat tggctaaatt tacttccgaa cctattttga
    13921 tgtgcggaat ttctttgggc ggaaaaattg tttatgattt tgcccataag tttccgcatt
    13981 tgtttaaagg ggccgttatc acagatgtga gtcccggctc tttcggtgaa tcccgattat
    14041 ttcaatttgt cgagggactt gtttcagatc tgaatttaaa tcaggactgg cccggcttaa
    14101 agaaggaatt gcgggagcgt attcccgagc ctagtttgag atccctcatt cagtctcagt
    14161 tgttttatcc cacgaaacag ccccctgcag cctggaaagt cggaatgaag cactttcggg
    14221 ccttgcttca acgtcaagcc atggatgagc agtttaaggc acttatgaag gtcgatgagc
    14281 ttttgctcaa acaaggaagc gaaattcgcg ttcttaaagc tgaaaatctg agtgccattg
    14341 atgacatctc tttcggagaa atgaagaaga tgaaatccat taagatttat cctcttatgg
    14401 gcgccagtca tttcattcat atcactcata aacaggatat tcaaaacctg ttaatgcagc
    14461 tgaaaacgag tgtctcagtt tgacccgaaa gatttcctga aaaagtcaat ttgaggcgaa
    14521 acaaatcgcc cctgacgttc gtcttatatc caaagacgtc gcaatccact tcatgtaagc
    14581 gcttggatcc ctcgtctgat cgttggctga actcgctttt tgaaaggagc aagttatgaa
    14641 gaggaataga gttttagttg cagcagtggt atttttaagt agcatgagct ttgctgaagc
    14701 tggtggcagt cgtcaagacg acagtttgga tcaagttcca tccatgtccg ggaaaattga
    14761 aatcatggaa gagcttggtc ttatagagca tcccagtcct tttcagcaga tctctcccag
    14821 gaaaacttct aattcgaaaa aagctgttcg ccaaaaagtt cgcatgggcc atttggcgga
    14881 tatctcagtt gatggcgaac attaaactcg tattcattta taaaggctgt tcaagtaaat
    14941 ataacggctc ctgccagggt ggcaggctct ctattttata gaagggtctg aaattttcaa
    15001 ctagacgaag atatggctat cataagtcgc gaaggcgctt attattcata ttaaaaatcg
    15061 tagcgcgact ttacttccga ttgggtacaa tcagaagtcc aatgaaaaca tttgcataat
    15121 tagtaattca tggaggattt ctatgacaaa gcgcttgagg ctaattctaa atttacgggt
    15181 tgctattttg cttctttcgt ccgcacagtt ttcttttgcc gcatctagct ctaactgacc
    15241 tgagaatcgc ttgggagatt atcaacagta ttgtcttagt ccatacgtaa ctgatgacgt
    15301 agccaaagca tgctatgacg tttgctttta taacgataga gattgttatg cgtacatcaa
    15361 gccatgttat gagtttggcg gaacatcaga aaaagtctat caatgtttta gaagtgccgg
    15421 cgatacgaat tctgatttta aggcttgctt aaagaattaa acgaacaaaa cccattgatt
    15481 gatattcgtg gaacagttga aacactcgcg gttagcgagc gtctgctttt agagaagagg
    15541 gatgacggct tttaggctct tggttaccaa ggctttgtct ttgtgagcga gctcaccgat
    15601 tttttctttg atgatctgat ggtctatggt gaaaagtttc atgcgcacaa ccgagggtgc
    15661 gggaagtccg caggatttaa gatcagtgac ttcaatatcc aggggccaag atttgttttt
    15721 tgcacttgta atcatagcgc acacactgtg tccggttttt ccgtcaaaat tatcagagtc
    15781 gctgagcacc agggccggtc gattttttga tttcggtgag tctgagtagg ggaaggggac
    15841 gacgatgact tcaaactgtt taaagcgagc cataggcctc atcatcttca ggtgagttcc
    15901 actcagataa agtcatctcg atggattttt gccactcaag atctattttg gagatttttt
    15961 taacgacgac gtgaccgtct tcgatttccc attggaggcc atcgccgctg gcgagcccta
    16021 agaaggcgcg aacttccttg ggaacagtgg cttggtattt ggaggtgagt tttgaggctt
    16081 tcataagtaa tacagtatta cattgttacg gtatacgcaa gtaaaatcga tctatttccg
    16141 cagtgtttac gatgagtctg gtgaaagagc attttaaggt ggcgtcggtc tcaaattgaa
    16201 gcatggcaga gttttaattt gaaccagttc ttaggggtgt taatctatgg atctagagct
    16261 tcatggggga agattttatg gaaaaggcac gaggtctgct gattttcttt cttttattca
    16321 cggtgacgtc ctgggctgag gaaagcagtt ttgagttggc gttgccagaa ctcgaatcct
    16381 catccccgtc gtccagtaat tttgttagtt cttttgagaa agaacccggg aataattttt
    16441 ctaacgaacc ctacgagtcc tcaacctctg ctcaaacttc cgctgaaccg gtgaaaggag
    16501 tttatgaaaa tgctttcact tcttatcagt caaaaaaaac tgccgaagag gagaacacag
    16561 cgactgatga gtggcgaaaa ggtctcgata aacaggataa ttttgattta gagaacctga
    16621 ccttttccga gccaaaaaca gccccacatc ctaggggtca agccaggaga cccactgttg
    16681 cttctgcaac cgacgtggct gaagataaaa tcccgatgcc tggacttgat aataaccagg
    16741 aaagcagtgc tcctccggtc tcagcgaagg atcgtaaagc ggcagtgatg aaaacgattt
    16801 cagataacta tgaggagctt aaagtttgct accaggaggg actcaaaaag aattccgaaa
    16861 tgaagggcaa agtcgtccta ggctggagta tggatacaca aggtcgtgtt tcgggggtag
    16921 aggtcctcac ttctcaattg aataataagc aggttgaaaa atgtatggca gagcatctcg
    16981 ccaattggca ttttcctcgt caagctaaac ttcgcagtag caaagatcgc atgacttatg
    17041 cttttcagtt tgtttctgaa aaagaataaa aataaataat ttaaagatag ttcttccctt
    17101 ttttcaccgc attggaaaaa agttttattt ttttatcttc aaatccgaaa atcagcgatt
    17161 gctgcggatt acctcacgcc attgggaatc aattgattat taattatttc agagttagcg
    17221 ttaaattgtc ggatttaact gtgattacga gatcacatta cctagtaact caatggggag
    17281 aatcaaatga ataaactagc tttgagctta attttcactt ttggaaccac tgttgcatat
    17341 gctggatcct ctaattctga taaagatatt tgggatgcat taaatgttga agtagagcaa
    17401 cttccgactc ttccaggtta ctccattggt gaggctaaac gtgtggggcg tctctcttgc
    17461 agtcatataa aacaagatgg atatgctgaa gttttcactt gttctttaaa agagttcgct
    17521 ggccattaaa acttgagcgg agatgctaaa atcggccagg tggtttttaa gggaacagcc
    17581 ttttctgaaa agatcaggac accatacttc ccataatgaa gtaagcgtgg agctaaggtc
    17641 tcaaggcccg gtcctcgtat ccaggcgaag atcaattccg gatggtttaa agagcgagcc
    17701 aagagagctg atttatattc acgtcgcaag taagaggttc caaataagat gatttcttcc
    17761 gggtttaatt taaaatcctg tccatgaagt tccttcgcca tgagcttttc atactggggc
    17821 gagtcgccga gcaggaggat ggtgcctttt ttaggcaggt ccaataggag cttatcctca
    17881 ctcagagtga tgggaattcc taaagcggct tgccagcttt tttgataaga cagtttttct
    17941 gtttcgttag tcccgactat ccacagatga tccgaaccaa aaacattgga aaagctcaag
    18001 ctttgttcct tgagatcaag ttcacggaag acatcaaagt caggatcgat ctcgagactt
    18061 tttggcgtgt ctcttaatac aaattgaaac ttcgcgatct tggattttac cctgaccagt
    18121 tcgttttgca ctttcccatc ggcaaagctc aagcgcactg gcacttctaa aacatagatt
    18181 tgtgggctgg tttgatggat ttcaaaattc actttaaatt tttgagctcc aagtttgctg
    18241 agttggactt gatgcaggct cagtttgggg actccggtac gatccagcca ttggttaaaa
    18301 aaacttttta agtctaaatg agaaatcgct tcaaaggttg tttctatctc ttcataactg
    18361 atgctttgac ctcggtggtt ccaataaaga cggcgaagtc cttgttcaaa taaaggagtg
    18421 cctagttgtt tcttcagcat atggaaaaac atcattcctt ttccgtagcc tacggcttga
    18481 gaaccaaagt taaagcgttc cttaaattgt ctgaggggaa agtcgtttgc cgatttcgca
    18541 taatcttgaa agttcatcag gctctggcgg cggtagtccc gatctgcccg cagagtttcc
    18601 tgctgccagt ggtccgccag ataagtggtg aggccttcgc accagttgcc gcggttataa
    18661 tcgacataaa cgctgttgcc ccaccaatta tgcaggagtt catggggtaa agaggaattt
    18721 aaaatataag gcaagcgaat gacgttcggt cccaaaagag taaaggccgg cattccaaat
    18781 ccggtctccc aaaagttttc aatcaccgca aaggattgat aggggaacgt tcccagggtt
    18841 ttttgataat gatcgaggta atcaggaaga aggctcagaa aagtttgagc cagttggggc
    18901 tccggatggc gcaaataaac tttgagggga ataccgctgg ggttattatc gagggcgtac
    18961 tcttgaaaat ctccggcaac caaataaata tcttgttgcg gaagagagag ctggtttaat
    19021 ggagaaacga tcttccaatt ttttggaaga tcacttttaa tgatttcgta agtggctttt
    19081 tccgaaaaat ccggaagcca ataagccgcg ccaaaaagaa tggctccctc aggggaaatc
    19141 aaacctgagg tgctgttatc agtaatgggc tcaaagatga ctccggaata ttgaaggtgg
    19201 acgaaatgat ctgcggcatc cgtttgtaat tcatattcat tataattggc ggcgggcttg
    19261 gtcgatggca acaagcgaag ctggtccccc ggagaagtga tcttcacctg gaggttttta
    19321 tttaaaagaa agtgatatct tttagaggcg gagagtgaca gctgatctag gatcatgagg
    19381 cttttcacgg caggggaaag agttacttcg agggcatggt gaatgccggc gaaggaggca
    19441 gaggaagtaa gagtcaaaag aaggctgatg cttgcgacga ctgttttcat aggctcctct
    19501 gttttaatat ttcatacagt gtttcatttt gatttgtcaa aatcatgggg gcctgtctaa
    19561 aaaaatttta ggttgtcgag gcattggtcg gctgtccaat ttattacttg taagcgtatg
    19621 aattacttcg agaattcctt tttattatga ggttttttag ttaaacatcg ttatgcttat
    19681 agataaaagg ggtttctatg agaatagcag ttgttcttgt gtcgctttgt tttttgttag
    19741 cttcttgtgc caccacctta tcaggggaag aagggactcc ttctgaggta agcccgcaaa
    19801 cagaggtgtc tcctccagtg cctttaccaa ctgaagtcac tcatgagact catgagtctc
    19861 aagaacccgt tgatctgggg tcccctaatt tggaatccca agcagccgct ccagagactt
    19921 cagcttctgg tttagatact ttttatgcta gcgaacaaca atcccctggg gtggatcaag
    19981 ttgctcccga aactcaagtg ccaccgacaa ttttaccaga aaaaatccat ttaaaaagaa
    20041 agtattttaa cgttgattct tcttatgagg atcagaaacc agaaaagcca gcttataaaa
    20101 agtcaaaaaa tttagccaag aagagggcca aagtttctaa gcgtgataaa aataaaattg
    20161 cgaaaaaca
//
LOCUS       DATBTE010000352         1985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_867680, whole genome shotgun sequence.
ACCESSION   DATBTE010000352 DATBTE010000000
VERSION     DATBTE010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867680"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /gene="tsaB"
                     /locus_tag="VIG33_13205"
     CDS             <1..523
                     /gene="tsaB"
                     /locus_tag="VIG33_13205"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY8271842.1"
                     /translation="VGQGPGSFTGIRVAANMGKSFSYIYNKPLVTIDSLVLLAAAIAT
                     ELPVLSIINAYKNMVYFGIFEPAKNPEPIYQQGPTAVPVQRLANMITQDVVVVGDGFE
                     TYQKYLPESLKIHLHRNSQFSDHPQAETLGLLAEKRVQQNKTIEWNSFVPLYIRASEA
                     EENKRGILISPLK"
     gene            539..1519
                     /locus_tag="VIG33_13210"
     CDS             539..1519
                     /locus_tag="VIG33_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEY8271843.1"
                     /translation="MERDFIKKSEDESQDVIAIKRPTQEKSSQIWVVGSGKGGVGKTF
                     VTTSLAICLSKMGHSVTIIDLDLSGANIHTAFGMPPATHSIRHFFEGSKNISELVILT
                     EIPRVSYIQGIWDAWTPTDISVEQIHQLVPEMRKLNSDFVLIDLGAGALASHLELMKI
                     ADEKILLSSPEPTSIEKTYRFIESFVCYSLKEYSVPEAYAHMVQTLRQNRNGFGEKNF
                     SFRNYLKQSSGIQPDYFETLMAKPLRLLVNSCRSQANSELGYSMKSVCYKYYDFKVDF
                     LGAIDFDNAVWQSIHNREPVLIAQPFTHLAGQFLTTCKQLIDPKELHAVI"
     gene            1528..>1985
                     /locus_tag="VIG33_13215"
     CDS             1528..>1985
                     /locus_tag="VIG33_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEY8271844.1"
                     /translation="MNIGSRNNYYDVLELSVNSAQYELAKAYQRAKATYSGDNPAIYT
                     IFSEQEARALLLLIEEAYSVLGNKNLRSIYDQRLLSGNFQMADLSYEAILKASKQMFP
                     EPRQEEKKNEYKNDPDFEKKITSQENWDGNFLKQVREYKNVTIRKMSDIT"
BASE COUNT          617 a          411 c          376 g          581 t
ORIGIN      
        1 tgttggacaa ggtcctggaa gctttaccgg tatccgagtc gccgccaata tgggaaagag
       61 cttttcttat atttataaca aacccttggt caccatcgac tctttggtct tattggctgc
      121 agccattgcc acggagttgc cagtcttatc tattataaac gcctataaaa atatggttta
      181 ttttggaatt tttgaacccg ctaaaaatcc agagcctatt tatcagcagg gaccaaccgc
      241 cgttccggtc caaagactag caaatatgat tactcaggat gttgtagtgg tcggcgatgg
      301 ttttgaaaca taccaaaaat atcttcctga aagtctaaag atccatcttc atcgcaactc
      361 tcaattttct gatcatcccc aagctgaaac tctgggtctt cttgccgaaa aacgagttca
      421 acaaaacaag accattgaat ggaattcttt cgtacccctt tatatccgag catctgaagc
      481 cgaagagaat aaaagaggaa tcttaatttc ccctttaaaa taacttcgga gtaagtccat
      541 ggaacgtgac tttatcaaaa aaagtgaaga tgaatcacaa gatgtgattg ctatcaaacg
      601 gcccactcaa gaaaaatcga gtcaaatttg ggttgtaggt tcaggaaaag gtggcgtcgg
      661 aaaaactttc gtcaccacca gcttggccat ttgcctcagt aaaatgggcc attcagtcac
      721 cattattgac ttagatttaa gtggcgccaa tatccatacc gcttttggaa tgccccctgc
      781 tactcatagc atccgtcatt ttttcgaagg ctcaaaaaat atttctgagc tggtaatact
      841 cacagagatt cctcgagttt cttatattca aggaatctgg gacgcctgga ctcctaccga
      901 tatttcagta gaacaaattc accagctcgt tccagaaatg aggaaattga actccgactt
      961 tgtacttatc gatcttggag ccggcgccct tgctagtcat ctggaattga tgaaaattgc
     1021 tgatgaaaaa attctcctct cttcgccaga acctacaagt attgaaaaaa cctatcgttt
     1081 tattgaatct ttcgtctgtt attcactaaa agaatacagc gttcccgaag cttatgctca
     1141 tatggttcaa actttacgtc agaatcgaaa tggttttggt gaaaagaatt tttcttttag
     1201 aaattattta aagcaaagct ctggaattca acccgattat tttgaaactt taatggctaa
     1261 accacttcgt ctgttggtaa attcctgtcg aagccaagct aactctgaac tcgggtattc
     1321 aatgaaaagt gtttgttata aatactatga ctttaaagtc gattttctgg gggcaatcga
     1381 ctttgacaat gcagtgtggc agtctattca caatcgtgag ccagttttga ttgctcagcc
     1441 ttttactcat cttgcaggcc aatttttaac gacatgcaaa caacttattg atccaaaaga
     1501 gcttcacgcc gtaatatagt attaaggatg aatattggtt caagaaataa ctattatgat
     1561 gttttagagc tgagcgtgaa ctcagctcag tatgaattgg ctaaagccta tcagagagcc
     1621 aaagccacat actctgggga taaccctgcg atctatacta ttttctccga acaagaagcc
     1681 cgtgcccttc ttcttctcat cgaggaagcc tattctgttt taggaaataa aaatcttcgc
     1741 agcatctatg atcaaagact attgagtggc aactttcaaa tggctgattt gtcttatgaa
     1801 gcaattctta aagccagcaa gcagatgttt ccagaacctc gccaggaaga aaagaaaaac
     1861 gagtataaaa atgatccgga ttttgaaaag aaaattacct ctcaggaaaa ttgggacgga
     1921 aattttctta agcaagtccg cgaatataaa aatgtcacta ttcgcaaaat gagtgatatc
     1981 acaaa
//
LOCUS       DATBTE010000353         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2439676, whole genome shotgun sequence.
ACCESSION   DATBTE010000353 DATBTE010000000
VERSION     DATBTE010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2439676"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VIG33_13220"
     CDS             complement(<1..846)
                     /locus_tag="VIG33_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271845.1"
                     /translation="MGKLTFAVPLLMFISQASYAEGMSSAYSFGGACASQGVWTQTAL
                     SATQNLRKVTLQLKDDANCKALGASLQASIQNIETAVQSASDTTARATRLSQLPREIN
                     ALRAFLNSAPDMKQQILRTMMDKSIESATLSAQVGPETDLISTNIGDFGSRMHRSANT
                     GLSLLNQVIDSIPNLSQCLMGEDQQALGSVMSTAVQMASAFASSGQDVTGSQLATTIS
                     KITSFVREQKYSKILRKLNQQEFLASMACLMEATSESYCQARTGMQFFQKGMEDLKVR
                     QNDKMD"
BASE COUNT          521 a          394 c          389 g          539 t
ORIGIN      
        1 atccatctta tcattttgac gaacttttaa gtcctccatg cctttttgaa aaaactgcat
       61 tcccgtgcga gcctggcaat aggactcgct ggtcgcttcc attaggcagg ccattgaagc
      121 caaaaactcc tgctgattta atttacgcaa aatttttgaa tatttctgtt ctcgcacaaa
      181 agaggtgatc ttggaaattg tcgttgcaag ctggcttccg gtcacatctt gccccgatga
      241 agcaaaagcc gaggccatct gcacggccgt gctcataacc gagcctagag cttgttgatc
      301 ctctcccatc agacactgac ttaaattcgg tatggaatct attacttggt tcaacaggga
      361 caatcctgta ttcgcactcc tatgcatacg ggagccaaaa tcacctatgt tggtacttat
      421 caaatcggtc tctggcccta cctgggcaga gagagtggca ctttcaatgg atttatccat
      481 catggttctt aaaatctgtt gcttcatatc cggagcactg tttaggaagg ctcttaatgc
      541 attaatttcc ctgggaagtt gggacaaacg cgtcgctcgt gccgtcgtat ctgaagcaga
      601 ttggacagcg gtttcgatat tttgaataga ggcttgaaga gaagcgccca aggctttgca
      661 gtttgcatca tccttcaact gcagcgtcac tttccgcaag ttctgggtgg cggacaaggc
      721 tgtctgagtc cagactcctt gagaagcgca agccccacca aaggagtaag ccgaagacat
      781 tccttccgca taggatgcct gagagatgaa catcaatagg ggcacagcaa aagttaattt
      841 tcccattcca tcaatactcg aaattttgtt gaatccattt tcaatacaaa actatcgaac
      901 tcctctggag tacttcttgc gataatcatt cctctatgag ctgtgtttta tcctttattg
      961 agtcaaaact tcgactaaaa tttagacagt gagacgggaa acggcgaatt gcaatacgga
     1021 tttctcgtag agaactttgg gacatcggcg gcagatttta tccaaatacg acggcctctc
     1081 cgttttttag tcttttagaa gagggtagtt tgtgccaact gatgatagaa aatatgcctg
     1141 accaagatcc attgtttttt atcaaaacaa gtctttattc tctccgaatg ttttcattgg
     1201 acttctggtt gtactcaaac agaagtaaag tcgcgctatg agtttcaaaa tgaataataa
     1261 gcgccttcgc gacttatgat agccgtatct tcgtctagta gaaaatttaa gacctttcta
     1321 taaacaccga gtacagcgct caatgattca aaacttcatc tagaaaaaag cttcgcagtc
     1381 aggaggtaaa atatattagg attcaaaaaa aattaagaag aagaacgaga aagaaagaac
     1441 tcaagaatat ttttcttacg tgagtttttt tgttgacgac aaaaaccctt ttctaaagct
     1501 tgatctatga ggtctttcct ttatctacct tgaaaaattt agataagccc aagatcttct
     1561 cggggcgaag gttttaagcc gcgatagagg ggagttaaag gttccttctg tcttgcccaa
     1621 aaagcgctcc tccagaagtc atgttatacc ttttctttca ggaggctttc tttgcggtaa
     1681 agtgattttg gtttttttaa aaagcagggg ctaaaatttg gcggggattt actggcgggg
     1741 aaaaggaaaa cggctcgacc tctttccacg aaaaagcccc tccatcttgt gttaaaaagc
     1801 acaggaagtt ctttctttag tcctggcaat cgacagattg aaa
//
LOCUS       DATBTE010000354         2945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1140354, whole genome shotgun sequence.
ACCESSION   DATBTE010000354 DATBTE010000000
VERSION     DATBTE010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140354"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="VIG33_13225"
     CDS             complement(<1..1245)
                     /locus_tag="VIG33_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEY8271846.1"
                     /translation="MANLTPELERERVKICKIAKDAGLDCFETIFELITYDQINQFAA
                     YGGFPVRYPHWKFGMEYEHLSKSYEYGLSKIYEMVINTDPCYAYLMEGNAMMDQKLVM
                     AHVYGHCDFFKNNIWFSKTNRKMMDQMANHATRIRRYVDRYGTETVESFIDICLSLEN
                     LIDRYSPYVDKSIVSKQPEAGNQDADLLLKVNRPYMRDYINPPAFVAEQKLKMQELAQ
                     KSQKYPAEPEKDILKFLIHHAPLADWQLDILSIIRDEAYYFSPQGMTKTMNEGWASYW
                     HSKLMTERILDDSEIIDFADHHSGTMAMAPNGYNPYKVGIELLRDIEERWNKGQFGRE
                     WEQCDDIRERKQWDKKLGLGREKIFEVRKVYNDVTFIDEFMTEDFCIRNKLFVYKFNK
                     KTNKFEIDTRDFKAIKQQLLFHM"
     gene            complement(1249..2343)
                     /locus_tag="VIG33_13230"
     CDS             complement(1249..2343)
                     /locus_tag="VIG33_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEY8271847.1"
                     /translation="MSIKEDHNRFKDIVKGKVKEDFKKYVTQGEMIGKKENEFVKIPL
                     PHIDIPTFRYGAKQQGGVGQGQGQPGEGVGEPGDGQGQAGENPGEHLLEVELSMDELA
                     DILGEKLELPRIEPKGNKSIEMEKTRYSGMAPVGPEGLRNFKASYKKALKRYVSSGIY
                     TPEDPVIVPIRRDFQYRSFKKVTKPQAKAVVIYMMDVSGSMGDEQKQIVRLESFWINT
                     WLKKHYKGLETRFIIHDAAAKEVDEDTFFRTSESGGTLISSAYKLCQQMIETDYPVNE
                     WNIYPFHFSDGDNWSGEDTRLCTKLLKEFFLPKTNVFCYGQVESKYGSGQFLKDLQKE
                     FGADERIICSQIENRDKILDSIKDFLGKGK"
     gene            complement(2353..>2945)
                     /locus_tag="VIG33_13235"
     CDS             complement(2353..>2945)
                     /locus_tag="VIG33_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HEY8271848.1"
                     /translation="LKNHSLLSNEELKGDYRELLGVVKQEYEEIIKAEVQRAISADES
                     ALLRLCANYIDNVKAFTQKEKVRNQFTGQDEEPDERLMRSIEDKIEIPESRKDDFRRE
                     IMNYIGALAVEGKKFNYKMNERLHKALELKLFEDQKDSIKLTSLVSSVTDKDTQEKID
                     IVKSRLIKDFGYDDVSATDVLHYVASIFARGDVKNK"
BASE COUNT          787 a          696 c          524 g          938 t
ORIGIN      
        1 catgtgaaac aaaagctgct gtttaatcgc tttgaagtct ctcgtgtcta tctcaaactt
       61 attcgtcttc ttattaaatt tataaacaaa gagcttattt cgaatacaaa agtcttcagt
      121 cataaactca tcgataaagg tcacatcgtt atagactttc cgcacttcaa agatcttttc
      181 gcggcctaac ccaagttttt tatcccactg ctttctttca cggatatcat cacattgttc
      241 ccactcacgg ccaaattgcc ccttattcca acgttcttca atatcgcgca ataactcaat
      301 ccctacttta taaggattgt agccatttgg ggccatggcc atggttcctg aatgatgatc
      361 cgcaaaatcg atgatttctg aatcatccag aatcctttct gtcataagct ttgaatgcca
      421 ataagatgcc cagccttcat tcattgtctt ggtcattcct tgaggcgaga aataataagc
      481 ttcgtcgcgg ataatcgaaa gaatatccaa ttgccaatcg gccaggggag catgatgaat
      541 caggaacttt aaaatatctt tctctggttc cgccggatac ttttgagact tctgggccag
      601 ttcctgcatt tttaacttct gttccgccac aaacgctggc ggattaatat agtctcgcat
      661 ataaggacga ttcaccttca acagaagatc agcatcctga tttccagctt ctggttgttt
      721 agaaacaatc gatttatcga cataaggact gtagcgatct atcaaatttt caagactcaa
      781 acatatatca ataaaacttt cgacggtttc cgtgccataa cgatcaacat agcggcgaat
      841 ccgcgtggcg tgattcgcca tttggtccat catttttcga ttggtcttgg aaaaccagat
      901 attattctta aaaaaatcac aatgaccgta gacatgggcc atcacaagtt tttgatccat
      961 cattgcattg ccttccatca agtaggcgta gcaaggatcc gtattaatca ccatttcata
     1021 aattttagat aagccgtact cataggattt tgaaagatgt tcgtactcca ttccgaactt
     1081 ccaatgtgga tacctcacgg gaaagccacc ataagctgca aattgattga tttgatcgta
     1141 agtgatcagc tcaaagatgg tttcaaaaca atccaagccc gcatcttttg ctattttaca
     1201 gattttaact ctttcgcgtt ctagttctgg cgtgagattt gccatgaatc acttcccttt
     1261 tcctaagaag tccttaatag aatcgagaat tttatcgcgg ttttcaatct gactgcagat
     1321 aattctttcg tcagcaccga actccttctg caaatccttt aaaaattggc cgctgccata
     1381 tttactttca acctgtccgt aacaaaatac attggttttt ggcaggaaga attctttgag
     1441 aagcttagtg caaagtcgag tgtcttcgcc gctccagtta tcaccgtcgc tgaaatgaaa
     1501 tggataaata ttccactcat tcactggata atccgtctcg atcatttgct gacacaattt
     1561 ataggccgaa ctaattaaag ttccacccga ttcgctggtt ctaaaaaaag tgtcttcatc
     1621 cacttctttg gctgcggcat catgaataat gaaacgcgtc tcgagacctt tataatgttt
     1681 tttaagccag gtattaatcc agaagctttc caaacgaaca atctgttttt gttcatcacc
     1741 cattgaacca gaaacatcca tcatatatat gacaacggcc ttagcttgag gtttggtgac
     1801 ttttttgaag gaacgatatt gaaaatcgcg acgtataggg acaattacgg gatcttccgg
     1861 agtataaatt cccgaactca cgtagcgttt caaggctttc ttataagagg ccttgaaatt
     1921 tcgaagtccc tctggaccca ctggcgccat tccagaataa cgggtctttt ccatttcaat
     1981 acttttattg cctttaggtt caatacgggg aagctcaagc ttctctccga gaatatcggc
     2041 aagttcatcc atgctgagtt caacttctaa aagatgttct cccggatttt ccccggcctg
     2101 accttgacca tctcctggct cgccaacccc ttctccaggt tggccctgtc cttgtccgac
     2161 gccaccttgt tgtttggctc catatcgaaa agttggaata tcgatatggg gaaggggaat
     2221 tttgacaaat tcattctctt ttttgccaat catttcccct tgggtgacgt actttttaaa
     2281 gtcctctttc accttgccct taacgatatc cttaaacctg ttgtggtctt ctttaatgga
     2341 cattgtttcc cactacttgt ttttgacgtc tccacgggca aaaatactcg ccacatagtg
     2401 gagcacgtca gttgcagaaa cgtcatcgta accaaagtct tttatcaagc ggcttttcac
     2461 aatatcgatt ttttcttgtg tatctttatc cgtcacagaa gaaaccaatg aagtgagctt
     2521 aatactgtct ttctggtctt cgaaaagttt caactccaag gctttatgca gacgctcgtt
     2581 cattttatag ttgaatttct ttccttccac cgcgagcgca cctatataat tcataatctc
     2641 acggcggaaa tcatctttac gcgattcagg aatttcaatt ttgtcctcaa tagaacgcat
     2701 caaacgctcg tctggctctt catcctgacc tgtaaattgg ttacgaacct tttctttctg
     2761 agtgaaggcc ttcacattat cgatgtaatt tgcgcataat cttaacagag cgctttcatc
     2821 cgcactaatc gccctttgaa cttccgcttt aatgatctct tcatattcct gcttaaccac
     2881 gcccagaagt tcgcggtagt cacctttgag ttcctcattg gaaagcaaag agtggttctt
     2941 aaggc
//
LOCUS       DATBTE010000355        21926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1415021, whole genome shotgun sequence.
ACCESSION   DATBTE010000355 DATBTE010000000
VERSION     DATBTE010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 21926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21926
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415021"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..460
                     /locus_tag="VIG33_13240"
     CDS             293..460
                     /locus_tag="VIG33_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271849.1"
                     /translation="MTKKRHSAWLVLFQILVVFIVSALFSKASFVANQKRADPQPKAK
                     SYALAIPESAE"
     gene            429..1772
                     /locus_tag="VIG33_13245"
     CDS             429..1772
                     /locus_tag="VIG33_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY8271850.1"
                     /translation="MPLLFRRAPSNLSCMEIRDPVHGSIYYSDAEVAVLDTAEYQRLR
                     AIKQLGFAEFSFPGATHNRYLHSVGVAHLAGLAFDSIFKVLPFSKSSTRLRLRQAFRL
                     AALLHDVGHGPLSHTTEQVMPHVESLGIKIYAQTNRPEIYQNRRANHEDYTIKYVTDS
                     MIGKTITRSFPDLSPVHVACLIDKSLQCPDDFFIDNGIDYRPILSQLVSSELDVDRMD
                     YLERDSLFCGTNYGKIDQPWILQNMTAHLEDGKMYLALNRRALYAFDDFLISRHHMYL
                     MVYFHHKSIIYEEMLNRYLTSKDCTFVLPSDIQEYTKYNDYKLHEHLASVSNPWAQRI
                     AQRKPYKVLIELHNTAEDRRAEAIKKMLEAEGIDSIWASSKTRLSKYHTASAEDRALQ
                     IFVVDQYDRFDKPAPIDQSTEIFTRYEGARIIDRLYVAPENYSAAEKILKVRPSF"
     gene            complement(1828..2487)
                     /locus_tag="VIG33_13250"
     CDS             complement(1828..2487)
                     /locus_tag="VIG33_13250"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8271851.1"
                     /translation="MPKKKFIRSSEFPYHVSARSNNREWFGLDPEGLWSIFSRYLYLV
                     QKLFNLEIHAFVMMSNHFHLLVKSPDANLDVAMRYFMRETSRVIARDLGRINHIYGGP
                     YHSSLICDPSYYQNAYKYVLRNPVTAKMCQRVEEYPYSTLQGLLGLRHLLIPTCANEE
                     LFYNTEEFLEWLNFAYQESENEVIRKALRKREFETYPSQKSRKKADFIPAPLSSHPVS
                     F"
     gene            complement(2599..3372)
                     /locus_tag="VIG33_13255"
     CDS             complement(2599..3372)
                     /locus_tag="VIG33_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8271852.1"
                     /translation="MEAGFNLHQRVALIAGPLSSTVSSIVMGLTRLGADCVILDPDKS
                     VPQAFISQISDAREINDKFGRAAFIPADLTSKDQIKDLIGRASQVFGGLDMYIDALMI
                     HQPTPMKIDEAIEGLDLMLDKHLKSSLYLTNGVLAFFKSRKRGRIIYLLNDSPVAKAK
                     EDILVHAVRSGLMKFSSALAKQVGEFNITVNALSIALTEEYILAHEPGTNSIKEAMEK
                     MKQLDPSLKITEPDKITQTVTFLVSPLGIAINGQTLNLS"
     gene            complement(3376..4560)
                     /locus_tag="VIG33_13260"
     CDS             complement(3376..4560)
                     /locus_tag="VIG33_13260"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEY8271853.1"
                     /translation="MEKIVFISGKRTPFGAFGGSLKDLSATDLGVASAKATLEQAKLP
                     ADKVDHVIFGSVIQSGVDSIYIPRHIGLKAGVPVGVGAFLVNRLCGSGFQSWISAAQM
                     IQTGEASVVLAGGTEQMSQVPYVVRGMRFGGIRLGNGELEDYMTAALVDAYSKTPMAI
                     TAENLAVKYGITREEVDKYSLQSQQRYQACFERGGFATEMCSVTVEGKKGSVVVDKDE
                     HPRPETNLEKLAQLKPVFKKDGIVTAAGSSGIVDGAACSLLTSESKAKELGLKPLARI
                     VSYASVGCEPSMMGIGPAGASRLALQRAGMKLEQMDLVEVNEAFAAQYLAVQKELGLD
                     PAKTNLNGGAIAVGHPLGASGTRIMNHLVYELQRRQLKYALGSACIGGGQGIAVIIER
                     MD"
     gene            4697..6931
                     /locus_tag="VIG33_13265"
     CDS             4697..6931
                     /locus_tag="VIG33_13265"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY8271854.1"
                     /translation="MTIDEKDSPASAIGKPVLRKEGPRKLTGQAKYVDDLDFKGLYGT
                     TVRSTIPRGKIKAIHFDEGIPWQEFTIVTAKDIPGKNYVALILHDQPYLADGVVNHPE
                     EPILLIAHSDKYLLERARQAVKIEYEKLPPVFDMQESLKAETVIWGKNNIFKSYLLEK
                     GDIESVWSKADFIFEDEYSTGAQEQLYIENNGMMAIANPNEGVTVWGSMQCPYYIHKA
                     LVPLFGLSEDKVRIIQTETGGGFGGKEEYPSILAGHAALLAWKSGKAVKIIYDRAEDM
                     VATTKRHPSRTRHKTAVSKDGRILGMDIQFLIDGGAYATLSSVVLSRGTIHAVGPYHV
                     ENVRIDAKAVATNCPPHGAFRGFGAPQSIFALERHLDQIAKKLNIAPEELRKRNFIKE
                     GEKTSTGQTIHEGVSMDALLDRALAQTHYYEKRQQFAKNNDHRKIKKGIGFASFMHGS
                     GFTGSGEKHLASKVGLRATAQGRVEILAASTEIGQGTNTILSQIAAETLNVSPDFIEI
                     SQPDTKHVPNSGPTVASRTTMVVGKLVQSAAIGLRQTLQQEGYLKNKYSEEELRQACA
                     SYIAAQGELQILVEYQAPPGIHWNDETYQGDAYGTYAWAVYVAEVSVDTTTWETKLDH
                     FLALQEVGRVIHPLLAAGQIEGGVAQGIGFTLYEKVKWKEGRMSNGSMTNYIMPTSSD
                     LPEIEVIFEEVPYKYGPFGAKGIGELPMDGPAPAILNAVAMATGREIKSIPMLPEDLM
                     RASL"
     gene            6928..7389
                     /locus_tag="VIG33_13270"
     CDS             6928..7389
                     /locus_tag="VIG33_13270"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8271855.1"
                     /translation="MKIDLKLNGKNVHVEAPPMKRLLDVLREELGVTGTKEGCGEGEC
                     GACSVMMDQILVNSCLVPIAQCAGADILTIEGLGTSEDLHPLQQAFLECGGAQCGICT
                     PGMLMASLELLRKNPAPNDQQIREGLAGNLCRCTGYMKIIDSVKKAAEVLK"
     gene            7386..8252
                     /locus_tag="VIG33_13275"
     CDS             7386..8252
                     /locus_tag="VIG33_13275"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEY8271856.1"
                     /translation="MKAFVPEYEIKTPENLNEVLQTLARSPGEWKVIAGGTDLMVTFE
                     TGKLAHKKFLNINSIKELKDLVVNEEDILIGAGCTYRQIQNHPIISEEFPLLVKAAQL
                     TGAIAIQNRGTLGGNIANMSPAADSPPALLAYDADLILLSTKDYRVLSYKYFHQGYRQ
                     CDLRPNEIILAIRLQRHQRMTHDYYRKVGTRSAQAISKVCMAATASIFRKQKIERIII
                     GLGSVSAVPLRATRTEHFLMQKPLTTDTFQEARKILLNEITPIDDIRSEALYRKEVAG
                     NLLTEFLESLRN"
     gene            8255..9061
                     /gene="xdhC"
                     /locus_tag="VIG33_13280"
     CDS             8255..9061
                     /gene="xdhC"
                     /locus_tag="VIG33_13280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HEY8271857.1"
                     /translation="MSWDWISELYSLKHKAQPVALVTIIKATGSTPRDIGTKLLVTDK
                     EFFGTIGGGTLEELIIEKARKLLKTPQAPERVPFPLCLKANQCCGGFVEVFIETLNTG
                     PQLIIFGAGHVAQSIAKTLEATAFQLHMVDNREEWLEKAAPSVTKYKNTGPEFIDQYE
                     HWNSEKTYVVVMTYDHDLDQDLIEKLLSKETRYLGLIGSNTKWQRFQKRLTEKGFPTE
                     SLSKVHCPIGLPIGGKAPQEVAISFAAEIIQLHNETFKALPRESWLENQL"
     gene            9058..9666
                     /locus_tag="VIG33_13285"
     CDS             9058..9666
                     /locus_tag="VIG33_13285"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY8271858.1"
                     /translation="MNFILLAAGFGKRMGGNKALMNFNGKPWALTQLQQIAEAGLTKT
                     LLVTNPDSAMALTKILSQIPSKFLPQTTLLTNPYPEKGPFSSLQLAIAAGLEESSFLC
                     PIDVPLSAKTLKIMRRSWRQTHEIEALIPLYQERKGHPVILSSNLQRSLLCLSSDSPE
                     ARLDFILKSLPENKKRILTIEDPNIIKNLNTPEDLIHEILAT"
     gene            complement(9681..10223)
                     /locus_tag="VIG33_13290"
     CDS             complement(9681..10223)
                     /locus_tag="VIG33_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271859.1"
                     /translation="MVRTALVILIIAVSQASLAKLNTEDTQPLTIPTQQENDKITQND
                     VAKIIPTDLQQTDDINLVAAKIADRSIQAWFNSAAVQSSPLGRTAKTVEQKMTTDITL
                     KADEPQAIEHKFSFQVLALQAMAKVQYTGWMNAVFNYDAKAAASMVEFSEKVFNKDLF
                     VNHSTSSKGNVSSVGLKWGW"
     gene            10653..11228
                     /locus_tag="VIG33_13295"
     CDS             10653..11228
                     /locus_tag="VIG33_13295"
                     /inference="COORDINATES: protein motif:HMM:NF025631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4360 domain-containing protein"
                     /protein_id="HEY8271860.1"
                     /translation="MFSTLFTTLMLGSLSAHASHRTVKVDSFSVAGSGCQPGTVSTAA
                     DAATGNYTVVPENFAAALTKGGSLDRKSCTFALAFQGTPHRAVRLTFPRIQGDVSLDE
                     NAAAEINYEVFFAGQSGDKGTLSFNGTQGILEQSFDEINTEQEVIFACGQSGIARGNA
                     SILVNSNPAGSQVAVAHVEEIGVKIDSIPCL"
     gene            11254..12015
                     /locus_tag="VIG33_13300"
     CDS             11254..12015
                     /locus_tag="VIG33_13300"
                     /inference="COORDINATES: protein motif:HMM:NF025752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HEY8271861.1"
                     /translation="MKDQKALRDYLQDALIQGRLRNPSYSLRALAQKAGLSPSALSEI
                     LSGKRRVTEERAQKILTKLGASPDHSDTILQSFSQTPIDQPQQQLSIDQFYLIAEWYH
                     FAILSLAETEDFKENPQWISKRLNLPIPQITKALERMERLGALTRDRKGHLRASGIQL
                     STPDEIAHRGLRQQHAEVLDLCRHSLEQHSISERDFLGVTMTLDPADIPLAKKLLRRF
                     VGKFCNRLEQGRKKEVYRLNLQFIPLTDVKEGKKK"
     gene            12012..13046
                     /locus_tag="VIG33_13305"
     CDS             12012..13046
                     /locus_tag="VIG33_13305"
                     /inference="COORDINATES: protein motif:HMM:NF025631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4360 domain-containing protein"
                     /protein_id="HEY8271862.1"
                     /translation="MKALHQLVISCVILSLSLSAQASVGHVTGNGGDHVRGMFLKMGE
                     AVVSFLQQTQEGQKLLLDNHMDPQSLQKLLDIQTVSVSETSLVDNGGSDVDAIGEKGK
                     IVLQKNRWMEHLEKERDVYYLVFHELLRALVINDDNYVISKSLLPFPKERRLTTRINP
                     LYPLIDSERLDKVFLLEQLTVNGSGCPLNQYGTRVDFDRESNQLDISFEEYNLPLKKE
                     SPELVARKSCSLAIPTELPPNTRLIVTQMDLTANLDLSKNSDLDFGGEVFFAGENGLR
                     MTQSVSGKDTAQKGRSLLRRNEVLKSACGGKGIFRVNTNGTLKRQNSADQTLAALGDF
                     KLSFKLESCQ"
     gene            complement(13086..13799)
                     /locus_tag="VIG33_13310"
     CDS             complement(13086..13799)
                     /locus_tag="VIG33_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271863.1"
                     /translation="MNIFKYFIIALTLFLGATSQADSAAIHGMVLFGKSTTYASHLPM
                     FHSPHDGQVIMKISLSDFPRCKTVLLYQKLSAAATDKIFTLLPEALDLAQVMNGEMTQ
                     FTASIYEGHFEKSGKLLGMVIVKVEKLILSAKLDSLAPEQNQYLIFGERGEYFAAHII
                     NGRPSYDSILEVKAPYRIKTGLCRLRYCPGLDEMTPVNDDQLPQAVPYVGGDLLGSPG
                     GTLAFINKSIYFEKEELAD"
     gene            13880..14587
                     /locus_tag="VIG33_13315"
     CDS             13880..14587
                     /locus_tag="VIG33_13315"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8271864.1"
                     /translation="MKITSHQLEAFFETAKLRSFSKAAQVLAVTQSALSQRIAHLEED
                     LEVTLFIRDPAGPLLTTAGELLLRHCQVTESLEQEVLGQLKSSSNQLRGSVRIAGFSS
                     VLRSVVIPSLAPFLRAHPQVHCEFSSHEMTELIDVLRSAEADFVVLDYSLQKKGIAEH
                     SLGKEEYGVIEGVELGLGRAVMSKHLLKENSQIKVVKGFKRYFREVTLNYFEQAYYSR
                     LHKEVVRQLTKNAPAYL"
     gene            complement(14605..15618)
                     /locus_tag="VIG33_13320"
     CDS             complement(14605..15618)
                     /locus_tag="VIG33_13320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY8271865.1"
                     /translation="MNSTGKKFTFFIVSNQTGQTRKLVISSVWMKVATFIGTVIILVC
                     VAGMVDYFGLLLQSLENKRLRTENTQLVKQFQVVESKVNSLENSLERVKTFTTKLKLI
                     TNIDAEDRVTKLSMGPKPAPGQQVEEYEPMEQRPSSESLAQQDAIFEQPQRLNESKGE
                     VAEQPAPSKDYASLVVRIDKAVHETQLKEQSVIDLWESLSERQSLINATPNVKPARGW
                     LTSRFGYRVSPFTSKAAMHAGLDIAAAPGSPVYAPADGVVSYAGYDQGYGKLVSIDHG
                     YGVSTRFGHTSQIYVQVGQKVSKWDVVAAVGNTGRSTGPHLHYEVRINGTPVDPINFI
                     LDE"
     gene            complement(15622..16383)
                     /locus_tag="VIG33_13325"
     CDS             complement(15622..16383)
                     /locus_tag="VIG33_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEY8271866.1"
                     /translation="MTQLEPQVTIDLMKIDAWFLTDKGLRRETNQDSCLINKEIGVYV
                     VADGMGGHSGGEVASAIAVQTIEDTLKRPDAQNLSPREVLAYAYEEASRRIYDKAATE
                     CPELAGMGTTMVMAYVRDGHIYIANVGDSRCYLFKKPYLWQLSEDHSLLNEQIRAGLL
                     KEENAGQFVARNVITRSVGYEREVFPDVIEREVIAGECYLLCSDGLSGLVSDQKISQI
                     LNQNSPDKAVKICVDQALANGGDDNVTVLILHFHE"
     gene            16472..17359
                     /locus_tag="VIG33_13330"
     CDS             16472..17359
                     /locus_tag="VIG33_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY8271867.1"
                     /translation="MKRSLTMALLLSVALHLLIFGGMAVYALATKPPLMPETVTLEIL
                     DQQPETKLEIIKEKTKTKIDKTSQIVEQEDKALNQDKVEDARYLSAHNQKVLKQTQAA
                     NHGEFQNLKTKTVKAGKPVENKKSALPSLNHLLAAYDPMATGFKKQEAKTNQEAEAAQ
                     KGGSVSQTSDYLKDIDQGIETLLNTREFKYYTYYNRIRKQLSQFWEPKVREKVNIMFQ
                     QGRKVASAQDRITKLLIVLNSRGTLVNVKVISDSGVRDLDDAAVEAFRAAAPFPNPPK
                     GIIETDGTVKIRWDFVLET"
     gene            17439..20267
                     /locus_tag="VIG33_13335"
     CDS             17439..20267
                     /locus_tag="VIG33_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase"
                     /protein_id="HEY8271868.1"
                     /translation="MSKPSDKNQIPKAISRETLESIARRAHYSATQMVFQANHRPDKE
                     KGDPKVGGHAAACASALHILGALHLIAKTGFDHIANKPHASPTDHAYNYLLDLFLKQD
                     LSKFSLDEANTALMGLRKYSHGEPVFQSYHSAYDSDHHNFFPSGTVGIPPVEAGYMAL
                     AYRYAREHGYEVPDAHFWALCGDSEFREGSMYEAVPDFAERELGSLTWIVDYNRQSLD
                     GQRITNPEIMNGTDADRIERTMAANGWEVIQVRHGAKRLALFKKPGGETLKTFLEKEL
                     EDYQLQALLLVQDMKALKKGIVKEYPNMKKFIDSVTDQELYEAIRDFGGHDILLLAEA
                     MEQSKKSTRRPTIIIAHTLKGWGLRCAAQPGNHSLLPSSEELNELKAKQGITGDNLFE
                     RFAASTPEAKFLKERGDKLWNDIKAQHSLKEKNQKFFLEKLTQWGDLPESLDINTKMT
                     SYPHTQWMLGQLTAKLTRIANTPLEENNLAEKQKALSAAEKPFKLPGELLISMAPDVG
                     TSTNLNPAMDGKIFGAPVVAEDLESELGVKDHKLPDLIPGEDVSDRFLRFEIAEGNVM
                     SCVGAFGRLRDIIGVPIIPLMTVYDFFIKRALDQYFYNLYWKSSFICVGTPSGVTLSP
                     EGAQHGWKSDIQIPNQITWEPFFCQELDWILCDAIKRHVMNNNAGRSGVLLRLVTRGI
                     EQKDMMYYLKKQARFKTDLSAKLVRAEFPVNGGTNEEEAVGMDEGQILHQVREEVLQG
                     AYYLIDYRGYAGYEPGDNVVNIFAMGAMVTEGIKASETLLGHGIYANVIAVTSPDLLA
                     GIQAHENDYTYLKHGLGINSDLYLRKSEDVSAGDLITVAGKRVPMVSVHDGEAGLLDN
                     LGSIIGVRHEACAVRKHSKCGRPSEIYAFHGIDAKSVVEACGKVLAETALEQVIVRDV
                     ALEQTQQAGKTTSHWTELWPTKNPMNKH"
     gene            20279..21139
                     /locus_tag="VIG33_13340"
     CDS             20279..21139
                     /locus_tag="VIG33_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271869.1"
                     /translation="MTTTVSLPYKGRLFKARKKKYNETVLFVPFYEATQRELARHVKF
                     VCDLGYDAVLFDLQKPPEILNFSEGILTPLKKWKELLMPFEVPINSQMKFGLKHVYAD
                     QIENMLNHIPGKKILFAFSNPGGAAIEALARRKCADVTGVIFDSGPSMKFVESFSNFV
                     HYNLNITPLILRYTLAPILSYAWSPDLHKDLHTDLAQFPQGFKVLSIRGWKDRLIPPD
                     HIDAVFEPHHNLDWRKLSLPEADHLLGLRDHRSEYAPVVKDFLHEISTQERTNFSNSA
                     KFRASACCKE"
     gene            complement(21076..21681)
                     /locus_tag="VIG33_13345"
     CDS             complement(21076..21681)
                     /locus_tag="VIG33_13345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobile mystery protein B"
                     /protein_id="HEY8271870.1"
                     /translation="MSDFKLEYAPGATPLNPNEIAGLKLSYISAQKELNAAEQDNILQ
                     GEKWAFAKKRKDFLTEKFMRDLHKRMFGHVWKWAGTYRTSDKSIGVDWYNIPTEMNKL
                     IGDVSYWITHETYPIDEIATRLHHRLVLIHPFPNGNGRWARLVSDVLLFSLGYKRFTW
                     GAKMKAHESLEEHSEVRAKYIHSLQQADARNFAELLKFVRS"
     gene            complement(21674..>21926)
                     /locus_tag="VIG33_13350"
     CDS             complement(21674..>21926)
                     /locus_tag="VIG33_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY8271871.1"
                     /translation="ETLQRAAHAMNCELVYALVPKDSLEEIVDEQAGKAAREILKRTV
                     HTMDLEMQGAGEAETTLHQEELARELKNKLDKRLWSLDE"
BASE COUNT         6098 a         5257 c         4821 g         5750 t
ORIGIN      
        1 tatataggac gaaataatga cggtccacca ggaatgatct ctgtttggag aggatggatg
       61 gtgctgatgc aatcggaaaa aacatggagg tcaattgaag cttttaattg tgggtaaaga
      121 taagctaaaa aacggagagg ctttgtaaaa ttctcccgat cgtcatggag ggtgacagaa
      181 tacgtccttt ttttgcgtct tctctcgaat acacaggact tcaggcgggc ccgcgctttg
      241 ctagatgctt tgtgaggccc gatagcccat aagcctattg aaggagtaat atatgactaa
      301 aaaaagacat tcagcatggc tcgtcttatt tcaaatctta gtggttttca ttgtctctgc
      361 cctttttagc aaggccagtt tcgtagccaa tcaaaaacgg gcagatcctc agccgaaggc
      421 aaaatcctat gcccttgcta ttccggagag cgccgagtaa tctttcttgt atggaaattc
      481 gagatcccgt tcacggttct atttactatt ccgatgccga agttgctgtc ttagatactg
      541 cggaatatca gcgtctcaga gccattaagc aactcggctt cgctgagttc agtttcccag
      601 gagcgaccca caatcgttat ttgcactccg tcggtgtcgc tcatttagcc ggtctggcct
      661 ttgattctat ttttaaagtt ttaccttttt ctaaaagttc gactcgtctt cgcttgcgcc
      721 aggcctttcg tctggcggct ttgctccacg atgtcggtca tggtcctttg agtcacacca
      781 ctgaacaagt gatgcctcat gtggaaagct tgggaataaa aatttatgct caaaccaacc
      841 gccctgagat ttatcaaaac cgccgggcta atcatgaaga ctatactatt aaatatgtga
      901 cggactcgat gatcggaaag acgatcactc gctcctttcc ggacctcagt cctgtgcatg
      961 tagcttgttt gattgataag tctttgcaat gccccgacga cttcttcata gacaacggca
     1021 ttgattaccg cccgatcctt tcccagctgg tcagctctga actcgacgtg gatcgcatgg
     1081 actatctgga acgggactct ctcttttgcg gaactaatta cggaaaaatt gaccagcctt
     1141 ggattttgca aaatatgacg gctcatcttg aggatgggaa aatgtacttg gccttaaacc
     1201 gtcgtgccct ttatgccttc gatgattttt tgatttctcg ccatcacatg tacctgatgg
     1261 tttatttcca tcacaaaagt atcatctacg aggaaatgct gaacagatat ttgacctcga
     1321 aagactgcac tttcgtcctg ccttcagata tccaagagta tacaaaatat aatgactaca
     1381 aacttcatga gcacttagcg agtgttagca acccctgggc acagcgaatt gcccaaagaa
     1441 aaccctataa agttctgatc gagctccata acacggcaga ggaccggcgt gcggaagcga
     1501 tcaagaaaat gcttgaagct gaaggtatcg acagtatttg ggcgagttca aaaacccgtc
     1561 tttcaaaata ccacacagcc tctgccgaag atcgagcgct gcaaatcttc gtggtcgatc
     1621 aatacgaccg cttcgacaag cccgccccga tcgaccaatc caccgaaatt ttcactcgct
     1681 acgaaggcgc ccgcattatc gaccgcctct acgttgcccc cgaaaactac tccgccgccg
     1741 agaaaatcct gaaggtacgg ccttctttct agtattgcag cgagttgtat ggtgtaaaat
     1801 cgaaatcgaa ttgacagaaa gttgtgatta aaatgaaaca ggatgggaag acaacggtgc
     1861 aggaataaag tcggcttttt ttctagactt ttgactgggg taagtttcaa attctctttt
     1921 tctcagtgcc ttacgaatga cttcattctc ggattcttgg taggcaaaat tgagccactc
     1981 aagaaactcc tctgtattat aaaataattc ttcatttgcg catgttggaa taagtaaatg
     2041 cctcaacccc aacaatccct gcaaagtact ataaggatat tcttccactc tttggcacat
     2101 tttcgcggtc actggattcc ttaatacgta tttataagcg ttctgataat aactcggatc
     2161 acaaatcaaa ctggagtgat agggtccgcc gtaaatatga ttaatccgcc ccagatcacg
     2221 agcgataact cggcttgtct cccgcatgaa gtaccgcatt gccacgtcca aatttgcatc
     2281 gggagattta accaaaagat gaaaatgatt gctcatcatc acgaaggcat gaatctcaag
     2341 gttaaaaagc ttctgtacaa gataaagata cctgctgaat atagaccaaa ggccttcagg
     2401 atctagtccg aaccactctc tattgttcga tctggccgat acatgatatg ggaactccga
     2461 acttcggatg aattttttct taggcattaa atactttctt tcaaaggatt taacacccaa
     2521 ctaagcgaaa atcaggccaa cgagtaggta cggccttctt tctagtattg cgctgcaata
     2581 ctagaaagaa ggccgtacct acgagagatt gagggtttgg ccgttgatgg ctatgcctag
     2641 ggggctgacg aggaaggtga cggtttgggt gattttgtcg ggttcggtga ttttgagaga
     2701 aggatcgagt tgcttcatct tttccatcgc ttctttgatg gagtttgtgc ccggttcgtg
     2761 ggccaaaatg tattcttctg tgagcgcgat cgacagggca ttcaccgtga tattgaactc
     2821 tcctacttgt tttgccagag ccgaagagaa tttcattaat ccagagcgca ctgcatggac
     2881 tagaatatct tccttggcct tggcaacagg agaatcgttt agaagataaa tgattcggcc
     2941 acgcttacgg cttttaaaga aagccaggac gccgttggtt aaataaagag agctttttaa
     3001 gtgcttatca agcatcaaat ccagaccctc gatggcttca tcaatcttca tcggtgtggg
     3061 ttgatggatc atcaaagcat caatgtacat atcaagtcca ccaaagacct gagaggctct
     3121 gcctattaag tctttaattt gatctttgga agtgagatcc gctgggataa aagcagctcg
     3181 accaaatttg tcgttgatct cgcgagcatc actgatctga ctgataaagg cctgaggaac
     3241 agacttgtca ggatctaaaa taacacagtc agcacccaat cttgtgagac ccataacaat
     3301 gctagaaact gtgctgctta aaggccctgc gatcagagcc actctttgat gcaaattgaa
     3361 tcctgcttcc actcactaat ccattctttc aataataacc gcgatcccct gaccaccacc
     3421 aatgcaagca ctgcccagag catatttcaa ttggcgacgc tgaagctcat acaccaagtg
     3481 attcatgatg cgagtgccgg aagctcccaa aggatgcccg accgcgatcg ctcctccatt
     3541 gagattcgtt ttcgccggat caagacccaa ctctttttgc acagccagat attgagccgc
     3601 aaaagcttcg ttcacttcca cgagatccat ttgctcaagt ttcatacccg ctctttgcaa
     3661 agctaagcgt gaagcccccg cagggccaat gcccatcatg ctgggctcgc aacccactga
     3721 agcgtaggaa acaattcgcg ccagaggttt taagcccaat tctttagctt tggactcact
     3781 ggttaacaaa gaacatgcag ctccatccac aattcctgaa gagcccgcgg ccgtgacgat
     3841 gccgtctttt ttgaaaacag gcttgagttg cgccagcttt tcaagattgg tctcgggacg
     3901 cggatgttca tctttgtcaa caaccacact gcccttttta ccctcaacag tgactgaaca
     3961 catctctgtc gcaaaaccac cacgctcgaa acacgcctga tagcgttgtt gcgattgcag
     4021 agagtattta tcaacctctt cacgagtaat accatacttc actgccaagt tttcagccgt
     4081 gatcgccatg ggtgttttag aataggcgtc caccaaagcc gccgtcatat agtcttccaa
     4141 ttcgccgttt cctaaacgga tcccaccgaa gcgcatgccg cgaacaacgt aaggcacttg
     4201 agacatttgc tcagtaccgc cggcaagaac cacactggcc tcccccgttt ggatcatctg
     4261 cgccgctgaa atccaagact gaaagccgct gccgcaaaga cgatttacca gaaaggcgcc
     4321 cactcccacg ggcacccccg ccttcaatcc gatatgacga gggatataga tcgaatccac
     4381 gccggattgg atcacacttc caaaaatcac atgatcgact ttatcggcag ggagtttggc
     4441 ttgttcaaga gtggctttgg ccgaagccac tcccagatcg gttgctgaaa gatctttcag
     4501 agagccacca aaagctccga aaggcgttct ttttccagaa ataaaaacta ttttttccat
     4561 ttcgctacac taataagaac tcaacagagg gtcaaacaat gccaaaaaat aattttttgt
     4621 tgtgtgtcgc aaacaaatct aatttaaatt agagtaaaaa attgattttt aaagagcaag
     4681 tggttcaata gataaaatga ctattgatga aaaagacagc ccagcttccg ctattggaaa
     4741 acccgttctg cgtaaagaag gccctcgcaa gctcaccggc caggcgaagt acgtagacga
     4801 tctcgatttt aaaggccttt acgggaccac cgtacgctcg acgattcctc gaggcaagat
     4861 caaggccatt cattttgacg aaggcattcc ttggcaggag tttaccatcg tcactgccaa
     4921 agacattcca ggaaaaaact atgtcgcgtt gatattacac gatcagcctt acttagccga
     4981 tggagtggtc aatcatccag aagaaccgat cttgctaatt gcccattccg ataagtattt
     5041 gctcgaacgc gcccgtcaag cggtgaagat cgagtatgaa aagctgccgc cagtttttga
     5101 tatgcaagaa tccctcaagg ctgagacggt catttggggc aaaaacaata tcttcaaatc
     5161 ttatctgtta gaaaaaggcg atattgagag cgtctggagc aaagccgatt ttattttcga
     5221 agatgagtat tcaaccggcg cgcaagaaca actctacatt gaaaacaatg gcatgatggc
     5281 catcgcaaac cccaacgaag gcgtcaccgt ctggggttcc atgcaatgcc cctattatat
     5341 tcacaaagct ctggttccgc tctttggttt aagtgaagat aaagtacgta tcatccaaac
     5401 cgagaccgga ggaggtttcg gcggcaaaga agagtatccc tcaatccttg caggccatgc
     5461 agcccttctt gcctggaagt ccggaaaagc ggtgaagatt atttatgatc gcgccgaaga
     5521 tatggtggcg accaccaaac gtcatccctc tcgcactcgg cacaaaacag ccgtcagcaa
     5581 agacgggcgc attctcggca tggatattca attccttatc gatggcggag cctatgccac
     5641 gctttcttcg gtagtgctct cgcgtggtac tattcatgcg gtgggcccct accatgtgga
     5701 gaatgtccgt attgatgcaa aagccgtcgc cactaactgc cctccccacg gagctttccg
     5761 cggcttcggg gcacctcaga gtatcttcgc tcttgaaagg catttggatc aaatcgccaa
     5821 aaaactcaat atagctcctg aggaattacg aaagcgcaat ttcatcaaag agggagaaaa
     5881 aacttccacc ggtcaaacca tccatgaagg cgtcagcatg gacgctctct tagatcgcgc
     5941 cctggcacag acccattact atgaaaaacg ccaacaattt gccaaaaaca atgaccaccg
     6001 aaaaataaaa aaaggcattg gctttgcgag tttcatgcac ggatctggtt tcaccggctc
     6061 cggagaaaaa catctcgctt ccaaagtggg ccttcgagcc acggctcagg gacgcgtgga
     6121 aattttagca gccagcaccg agatcggcca aggaacgaac acgatccttt cgcagatcgc
     6181 cgcagagact ttaaacgtca gtcctgattt catcgagatc tcccaacctg acactaaaca
     6241 cgtgccgaac tctggcccca ctgtggcttc tcgcaccacc atggttgtgg gcaagcttgt
     6301 gcaatccgcc gccattggcc ttcgacagac tttgcaacaa gaaggctatc ttaaaaacaa
     6361 atacagcgaa gaggagcttc gccaagcctg tgcgagctac atagccgctc aaggcgagct
     6421 ccagatcctt gtggaatacc aagctcctcc cggaattcac tggaacgacg agacctacca
     6481 aggtgacgct tacggcactt atgcctgggc tgtttacgtc gcggaagtca gcgtggacac
     6541 caccacttgg gaaaccaagc tggatcattt tttggctttg caagaagtgg gacgagtcat
     6601 tcatccgctc ctggccgctg gccaaatcga aggcggagtg gctcaaggaa tcggctttac
     6661 tctttacgag aaagtgaaat ggaaagaagg tcgcatgagc aatggatcca tgaccaatta
     6721 tattatgccg accagcagtg acctgccgga aatagaagtg atctttgaag aagtgcccta
     6781 caaatacggc ccctttggcg ccaaaggcat tggcgagttg cccatggatg gtccagcccc
     6841 tgcgattctc aatgcggtgg ctatggccac gggtcgcgaa attaaatcca tacccatgct
     6901 accagaagat cttatgagag cgagtttatg aaaatagatc ttaaacttaa tggaaaaaac
     6961 gtccatgttg aagctccgcc catgaagcgg cttttagatg ttttgcgtga agaactcggt
     7021 gtcacaggaa ccaaagaagg ctgcggcgag ggagagtgcg gagcttgtag cgttatgatg
     7081 gatcagatcc tggtgaacag ctgcctggtt cccatcgctc aatgcgcggg agcggacatt
     7141 ctcactatcg aaggtctggg aacctcagaa gatctgcatc ctctgcaaca agctttctta
     7201 gaatgcggag gcgcccagtg cggaatctgc actccgggaa tgctcatggc aagccttgag
     7261 cttttgagaa aaaaccccgc tcccaatgac caacaaattc gcgaaggact tgcaggaaat
     7321 ctctgccgtt gcacaggata tatgaaaata attgattccg taaaaaaagc tgccgaggtt
     7381 ctgaagtgaa agcttttgtc cctgagtatg aaataaaaac tcctgagaat ttaaatgagg
     7441 ttctgcaaac tcttgcccgg tctccaggag aatggaaagt gattgctggc ggcacagatt
     7501 tgatggtcac atttgaaacc ggcaagttag cacataaaaa atttctcaac atcaattcta
     7561 tcaaagaact caaagacctg gtggtcaacg aagaggatat tttgatcgga gcaggttgca
     7621 cctaccggca gattcaaaat catcccataa ttagcgaaga atttccgctt ctggtaaaag
     7681 cggctcagct aaccggtgcc atcgcaattc aaaatcgtgg aaccttgggg ggaaatatcg
     7741 ccaacatgag cccagccgcg gactctcctc ctgctttatt ggcctatgat gcggacctga
     7801 ttctcttatc caccaaggac tacagggtac tttcctacaa atactttcat caaggctatc
     7861 gacagtgtga cttaaggccg aatgaaatta tcttggccat tcgcttacaa cgccatcaac
     7921 gaatgactca tgattattat cgcaaggtgg ggacgcgaag tgcccaggcg atttcgaaag
     7981 tgtgtatggc cgcaaccgcc tctattttcc gcaagcaaaa aatcgaacga atcattattg
     8041 gccttggcag tgtcagtgcg gttcctttgc gagcaacaag aaccgaacat ttccttatgc
     8101 aaaagcctct cactacagac actttccaag aagcgcgtaa aattttgtta aatgaaatca
     8161 ctccgattga tgatattcgt tctgaagctc tttatcgcaa agaggtggcc gggaatctat
     8221 tgacagagtt tctggaaagt ttaagaaact aagcatgtct tgggattgga tttctgaact
     8281 gtacagttta aaacacaaag cgcaacccgt cgctttggtg acgatcatta aagcaacggg
     8341 gtctactccc agagatatcg gcactaagct tctcgtcacc gacaaagagt tctttggcac
     8401 aattggcgga ggcaccctcg aagaactcat cattgaaaaa gcgcgaaagc ttttgaagac
     8461 cccacaagct ccagagagag tgcctttccc cctctgcctt aaagccaatc aatgttgcgg
     8521 aggttttgtc gaagtcttta ttgaaacttt gaatacaggc cctcaactca tcattttcgg
     8581 agccggtcac gttgctcaat ccattgccaa aacccttgag gccaccgcat ttcaattgca
     8641 tatggtggac aatcgagaag agtggctgga aaaggccgcc ccctcggtga ctaaatataa
     8701 aaatacaggc cctgaattta ttgaccagta cgaacattgg aattctgaaa agacttatgt
     8761 cgtagtgatg acctacgatc acgatctcga tcaagattta atagaaaaac tcttgagcaa
     8821 agaaaccaga tatctgggcc tgatcggcag caataccaaa tggcagcgtt ttcaaaaacg
     8881 gcttacggaa aaaggatttc ccacagaaag tctctccaaa gtgcactgcc caatagggct
     8941 tcccattggc ggaaaagctc cacaagaagt ggccatcagt ttcgcggcag aaattattca
     9001 attgcacaat gaaactttta aagctttgcc gagggagtca tggctggaga accaattatg
     9061 aatttcatct tgctcgcagc aggattcggc aagagaatgg gcgggaacaa agctctgatg
     9121 aatttcaacg gaaagccctg ggccttgact caacttcagc aaattgccga ggccggtttg
     9181 acaaaaaccc tcctggtgac aaatccagat tcggccatgg ccctgacaaa aattctttct
     9241 caaattcctt ctaaatttct gccacaaacg acccttctca ccaatccata tccagaaaag
     9301 ggacctttct cttcattgca gttggccatc gcggcagggc tcgaagagtc cagttttctc
     9361 tgtcctatag atgtcccttt aagtgcaaag actctcaaaa taatgcggcg atcctggcgg
     9421 caaacccatg agatcgaagc cttgattccc ttatatcagg agcgcaaagg acatccggtg
     9481 atcttatctt cgaatctaca aagaagcctg ctttgtcttt cttccgacag tcccgaagcg
     9541 cgcttggatt ttatattgaa atctcttcca gaaaataaaa agaggatact cactattgag
     9601 gatccaaaca tcatcaagaa tctcaatacg cctgaagatt taatacatga aattttagca
     9661 acataagcaa gaagtgttaa ctaccagccc catttaagac ccacggagct gacattgcct
     9721 ttggaggaag tggagtgatt gacgaataag tctttattga aaactttttc tgaaaactca
     9781 accatggatg ccgccgcttt tgcgtcgtag ttgaacacag cattcatcca acccgtgtac
     9841 tgaactttag ccattgcttg tagagccaaa acttggaatg agaatttatg ttcaatagcc
     9901 tggggctcat ctgctttcaa agtaatatcc gtcgtcatct tctgctcaac cgttttcgcg
     9961 gttcgcccta aggggctgct ctgaaccgca gctgaattaa accacgcttg aatgctgcgg
    10021 tctgcgattt ttgcagctac gagattgata tcatcggtct gttgaagatc cgtcgggatt
    10081 attttcgcaa cgtcattttg agtgatttta tcattttcct gctgggtggg aatagttaat
    10141 ggctgagtat cctctgtatt caacttcgcc agagaggctt gagaaactgc aataataagg
    10201 attaccaggg ccgttctaac catacctagg actacttcaa aagtcaggcc aggctctttt
    10261 tgcacttaaa tgcaattaaa cggtttcccg tccccatctc gatttaatgg tgactacttt
    10321 tgcttcagca tatgttagct tttacataat tttcataaat gtcatcattg actcatagta
    10381 aaacatcttc tgtgcaaaat tgagaatttt taggaaatta agatgaattt tagacttata
    10441 ttggagatca gtaacttatc aataggatcg atttttttta aaaagagctg tgactaaaaa
    10501 tacgtcacat ctcatggaat cagccacctg gcagccgcct gttccaagct tggaacactt
    10561 tgttccaatt taaattgacc tccccttctc agtcacataa acctaagttt agaaaacgct
    10621 gtacttacat ggcgttgcac aagaggggtg ttatgttttc gactttattc acaactttaa
    10681 tgcttggatc tctgtcagct cacgcatcac atcgcactgt taaagtggac tcgttttctg
    10741 tcgcaggctc tggctgtcag ccaggaactg tgtcaacggc agcagatgcg gccactggaa
    10801 attatacagt cgttccggaa aatttcgctg cggctctcac taagggtggg tctttggata
    10861 gaaagtcctg cacctttgcc cttgcctttc aagggactcc tcatcgcgcg gttcgtttga
    10921 ctttcccacg gatccaggga gatgtgtctc tcgatgaaaa tgcagcagca gaaatcaact
    10981 atgaagtctt ctttgctggt caaagcggag acaaaggcac cctttccttt aatggaactc
    11041 aaggaattct tgagcagtct tttgacgaga tcaatacgga acaagaagtg atctttgctt
    11101 gtggccaaag cgggatcgcc cgaggcaatg cttccatctt agtcaatagc aaccctgcgg
    11161 gcagtcaggt tgccgtggct cacgtggaag aaatcggagt taagatcgac tcaattccct
    11221 gtctttaatt tgcaacgagg aatttgcatc gttatgaaag atcagaaggc tttaagagat
    11281 tatttgcaag acgcccttat tcaagggcgt ctgcgcaatc ctagttactc cctcagagcg
    11341 ctggcacaaa aggccggcct cagtccttct gccttgtcag aaattctcag tggcaaacgt
    11401 cgcgtcactg aagaacgggc gcagaaaatt ttaacgaaac tgggtgcgtc gccggaccat
    11461 tcagacacaa ttctacagtc gttttctcaa acaccaatag accaaccaca gcagcagctt
    11521 tccattgatc aattttattt aattgcggag tggtatcatt ttgccattct ctcattggct
    11581 gaaacagaag actttaaaga aaatccacaa tggattagca aacgactaaa tctacccata
    11641 cctcaaatca ccaaagctct ggagcgaatg gaacgattgg gtgccttaac tcgtgaccgc
    11701 aaaggccatc ttagggcctc tggtattcag ctttccactc ctgatgaaat tgcccatcgc
    11761 ggcttaagac aacaacacgc cgaagtctta gatctgtgtc ggcattcatt ggagcaacac
    11821 tctatttctg agcgagattt tcttggagtc acaatgactc tggatcctgc tgacattcct
    11881 ctggcaaaga aattgcttag aagatttgta gggaagtttt gcaatcgact tgagcaagga
    11941 cgcaaaaaag aagtttatcg actcaatctg caattcatac ctttgactga cgtcaaggag
    12001 gggaagaaaa aatgaaagca cttcatcaac tcgtcatttc gtgtgttatt ctgagcttga
    12061 gcttgagtgc gcaagcgtcc gtcggacacg tcactggaaa cggtggcgat catgttcgcg
    12121 gcatgttctt aaaaatgggt gaagccgtcg tctcttttct gcagcagacc caggaaggcc
    12181 aaaaactgct tctcgataac catatggacc ctcaatctct tcagaaactc ttagatattc
    12241 aaactgtctc ggtgagtgaa acctctctcg tagacaacgg aggctccgat gtggatgcca
    12301 tcggcgaaaa agggaaaatc gttttacaaa aaaaccgctg gatggaacat ctcgagaaag
    12361 aacgcgatgt atactatctc gtattccatg aattactgcg tgcgcttgtc attaatgatg
    12421 acaattatgt catctctaaa tcccttttac cctttccgaa agagcgacgc ctcactacgc
    12481 gtattaaccc tctttatccg ttgattgatt ctgaacgctt ggacaaagtc tttttacttg
    12541 agcaattgac cgtcaatggc agtggctgcc ccttaaatca gtatggcacc cgagtggatt
    12601 ttgatcgtga atctaaccag ctcgatatct ccttcgagga gtacaacctt cctttgaaga
    12661 aagagtcgcc agagcttgtt gctcgcaaga gttgttcact ggcgataccc acagagcttc
    12721 ccccaaacac ccgcttgatt gtcacgcaga tggatttaac cgcgaacttg gatctttcca
    12781 aaaattcgga tcttgatttt ggcggagagg tcttctttgc cggcgaaaat ggacttcgca
    12841 tgactcagtc tgtttccgga aaagacacag cccaaaaagg ccgcagctta ctgcgtagaa
    12901 atgaagtctt aaaaagcgcc tgtggcggca aaggcatctt cagagtgaat accaatggaa
    12961 ccttaaaaag acaaaactct gcagaccaaa cactggcggc cctcggggat tttaaactga
    13021 gcttcaaatt ggaaagctgc cagtaaaaaa atgaagagtt tttattaaag cgcagaagct
    13081 ctagatcagt ctgccaactc ttctttttca aaataaatag atttattgat aaatgcgagg
    13141 gtgccaccgg gacttcctaa gagatcgcct cccacgtagg gcaccgcttg gggcaactga
    13201 tcgtcattga ctggagtcat ttcatcaaga ccgggacagt atcgcagtct acagaggccg
    13261 gttttgattc tgtaaggcgc ttttacttcg agaatcgaat cataactggg ccgcccattg
    13321 attatatgag ctgcaaaata ttctcccctt tctccgaata tcaaatattg attctgctca
    13381 ggcgccagtg aatcgagttt tgcagacaag atcaattttt ccaccttaac aataaccatg
    13441 cctaagagtt taccgctctt ttcaaaatgt ccctcataga tcgaggcagt gaattgagtc
    13501 atttctccat tcatcacttg ggccaaatcc agtgcttccg gaaggagagt gaatattttg
    13561 tctgtggcgg cggcgcttaa cttttgataa agaaggactg tcttacatct tgggaagtca
    13621 gaaaggctta ttttcatgat gacctgtcca tcatggggtg aatgaaacat cggtaagtgt
    13681 gaggcataag tggtggactt gccaaaaagc accattccat gaattgccgc ggagtccgcc
    13741 tgggaagtcg cccccagaaa aagcgtgaga gcgatgataa aatacttgaa gatattcata
    13801 ttttttcctt tattgagagg ctcacagaga gcttgttttt gttgaaagca agatagagca
    13861 aaacctaaat ttaataagca tgaaaattac atcacatcag ttagaggctt tttttgaaac
    13921 ggctaagcta agaagttttt cgaaggcagc acaggtcctt gctgtgacgc aatcggcgct
    13981 ttctcaacgg atcgctcacc ttgaagagga tttagaggtc acacttttta ttcgagatcc
    14041 agcgggtccc cttttaacga cggcgggaga gctgcttttg cgccactgcc aggtaacgga
    14101 gtctttagag caagaagttc tggggcaatt aaaatcttcc tcaaatcaac ttcggggttc
    14161 agtgcgaatt gccggatttt cttccgtatt gcgttcggta gttataccaa gtcttgcccc
    14221 tttcctgcgt gcccatcctc aagttcattg tgaatttagt agccatgaaa tgacggagct
    14281 cattgatgtc ctaagaagtg cagaagccga ttttgtggtt cttgattact ccttacaaaa
    14341 gaagggaatt gcggaacata gtttaggcaa agaagagtat ggcgtcattg aaggcgttga
    14401 gcttggcctt ggccgggccg tgatgtcgaa gcacctcctg aaagaaaatt cccaaatcaa
    14461 agtcgtgaag ggctttaagc gctatttccg cgaggtcacc ttaaattact ttgaacaagc
    14521 ctattactcg cgcttacata aagaagtggt tcggcagctg accaaaaatg ctccggcgta
    14581 tttgtaggat acctcttacc cagatcattc atctaagatg aaattgatgg gatccactgg
    14641 cgttccgttg atgcggacct cataatgcaa atgaggtccg gtggagcggc ctgtgttccc
    14701 caccgccgcg accacatccc atttggaaac tttctgtcct acttgaacat agatttggga
    14761 agtatgacca aaacgagtgc tcactccata accatgatct atactgacca gttttccata
    14821 cccctgatca taaccggcgt aactcacaac cccatccgct ggcgcgtaaa caggagaccc
    14881 cggagcggca gcaatatcaa gacccgcgtg catggccgct ttgctagtaa atggagagac
    14941 ccgatagccg aagcgcgagg tgagccaacc ccgcgctggc ttaacattgg gagtggcatt
    15001 aattaaactt tgtctttctg acaaactctc ccaaagatca atcacacttt gttctttaag
    15061 ctgtgtttca tgaactgctt tatcaatacg cacgactaaa gaagcataat cctttgaagg
    15121 agcgggctgc tcggccactt ctcctttact ttcattcaac ctctgcggtt gctcaaagat
    15181 cgcatcctgc tgagccaagc tctccgagga gggccgttgc tccataggct cgtactcttc
    15241 cacttgctga ccgggagcag gcttgggacc catcgatagt tttgtcacgc gatcttcagc
    15301 atcaatattg gtgatgagtt tcaatttagt cgtaaaagtc tttacacgtt caaggctgtt
    15361 ctcaagtgaa ttcaccttgc tctcaacaac ttggaactgt tttaccaact gagtattctc
    15421 tgtgcgcaag cgtttatttt caagcgattg caaaagcaag ccgaaataat ccaccattcc
    15481 cgcgacacaa actaaaataa tcactgtacc gataaaggtg gccactttca tccatacgga
    15541 ggaaatcacc aacttacgag tttgcccggt ttgattacta actataaaaa aagtgaattt
    15601 ttttccagta ctgttcatta cctactcatg gaagtgcaaa attaaaacgg tgacgttgtc
    15661 gtcgccccca ttcgccaacg cttgatctac acaaattttc acagctttgt caggagaatt
    15721 ctgatttaag atctgcgaaa ttttttgatc tgacactaaa cctgaaagtc catcggaaca
    15781 aagcaaataa cactcccccg caatgacttc ccgctcaata acatccggaa acacttcgcg
    15841 ctcatatcct acgctccgag taatcacatt acgagcgaca aactgaccgg cattttcttc
    15901 ttttaaaaga cccgcacgaa tttgttcatt caatagcgaa tgatcttcac tcagttgcca
    15961 caagtaaggt ttcttaaata gataacatcg agaatcccca acattagcta tataaatatg
    16021 tccatcgcgg acataggcca tcaccatggt ggtccccatc cccgcaagct caggacattc
    16081 ggtagcggct ttatcataga tgcgacgact ggcctcttca taggcataag ccaaaacttc
    16141 gcgcggagat agattttgag catccggtct tttaagagta tcttcgatag tttgcacagc
    16201 aatcgcagag gccacttcac ctccggaatg tccccccatt ccatccgcaa cgacgtatac
    16261 accgatctct ttatttatca gacaagaatc ttgattggtc tcacgtcgaa gacccttgtc
    16321 tgtcaaaaac catgcatcta ttttcatcaa atctattgtg acttgtggtt ctaattgagt
    16381 caaactagac tcttgtcgaa ttctttcatc ttgtttaatc caaacctaca tttcgcgtgt
    16441 cctaataccg atagagctgc gaggcttgtt gatgaaacgt tctcttacta tggcgctact
    16501 actttcagtc gccctccatt tattgatttt tggaggaatg gcggtttatg cgctcgcgac
    16561 aaagcctcca cttatgcctg aaacggtgac tctcgaaatt ctcgaccaac agccagaaac
    16621 aaaattagaa attattaaag aaaaaaccaa aactaaaatt gataagacct cacaaatcgt
    16681 ggaacaagag gataaagctc taaatcaaga caaggttgag gatgctcgct acttgagcgc
    16741 tcacaaccaa aaggttttaa agcaaactca agctgcgaat catggtgaat ttcagaatct
    16801 aaaaacaaaa acggtgaaag ctggtaagcc agttgaaaac aaaaaatccg cactgcccag
    16861 tttaaatcat ctccttgctg cttacgatcc catggccacg ggctttaaaa aacaggaagc
    16921 caaaacgaac caggaagctg aagccgcaca aaaaggcgga agcgtcagtc aaacaagtga
    16981 ttacttgaaa gacatcgatc aaggcatcga aaccttactt aatacacgtg aattcaaata
    17041 ttacacctac tacaatcgta ttcgtaaaca gctctcgcaa ttttgggagc ccaaggttcg
    17101 cgaaaaagtg aacattatgt ttcagcaagg acgtaaagtc gcttcagcgc aagatcgtat
    17161 caccaaactt cttattgtat taaactcaag gggcaccttg gtgaatgtaa aagtgatctc
    17221 cgactcaggt gtgcgcgatc ttgatgatgc cgcagttgaa gcgtttaggg ccgcagcgcc
    17281 atttcctaat cctcccaaag gcattattga aactgatggc acagttaaaa tccgctggga
    17341 ttttgtatta gagacttaac tctatgtcgc cccaagtcac ttctttgttc tatgatagcc
    17401 tgatgttaaa gatgtccttt atttgagagg agaaaacaat gagcaaacca tctgataaaa
    17461 accagattcc gaaagctatt tctcgcgaaa ccttggagtc tatcgctcgc cgtgcccact
    17521 actccgcaac gcaaatggtt ttccaagcta atcatcgccc cgacaaagaa aaaggggatc
    17581 caaaagtggg cggtcatgct gcagcctgcg ccagcgcttt gcacatcctg ggcgctttgc
    17641 atttgattgc taaaacaggt ttcgatcata tcgccaataa gccccatgct tcacccaccg
    17701 atcacgctta caattatttg ctggatttat tcttgaaaca ggacttgagc aagttttctt
    17761 tagacgaagc caacactgca ttgatggggc ttcgcaaata ctctcatgga gaacccgtat
    17821 tccaatccta ccactccgct tatgattctg atcatcacaa cttcttccct tcaggcaccg
    17881 tcggcattcc acctgtagaa gccggatata tggccttggc ttatcgttat gctcgcgagc
    17941 atggttatga agttcctgat gctcactttt gggctctgtg cggagattct gaattccgcg
    18001 aaggttccat gtacgaagcc gtgccggatt ttgcggagcg cgaactcgga tctttaactt
    18061 ggattgtgga ttacaaccga caatctttgg atggacaacg tatcaccaat cctgaaatca
    18121 tgaatggcac cgatgcggat cgtatcgagc gcaccatggc cgccaacggt tgggaagtta
    18181 ttcaagttcg ccatggtgcc aagcgtttgg ctctctttaa aaaacccggc ggtgagactt
    18241 tgaaaacctt cttggagaaa gaacttgaag actaccaact tcaagctttg cttcttgttc
    18301 aagacatgaa ggctttaaaa aaaggcatcg ttaaagaata tcccaacatg aagaaattca
    18361 tcgacagcgt gactgatcag gaattgtatg aagccattcg tgattttggg ggccatgaca
    18421 tcttgttact agcggaagcg atggagcagt ctaaaaaatc cactcgtcgt cccaccatta
    18481 ttatcgcgca taccctgaaa ggttggggcc tgcgttgtgc cgctcaacct ggaaaccact
    18541 ccctgctgcc aagttcagaa gaattgaacg aactaaaagc aaagcaaggc attaccgggg
    18601 ataacttatt cgaacgtttc gccgcgtcga ctcctgaagc aaaattcttg aaagagcggg
    18661 gtgataaact ttggaatgac atcaaagccc agcactcctt gaaagaaaaa aaccaaaagt
    18721 tcttcttaga aaaactcacc cagtggggag accttcccga gtctttagat atcaatacaa
    18781 aaatgaccag ctatccccac actcagtgga tgctggggca gttgacggca aaactcactc
    18841 gtattgcgaa tacacctctg gaagaaaaca acttggctga aaagcaaaaa gctctcagtg
    18901 ccgctgagaa acctttcaaa cttccaggtg agttgttgat ctcaatggct ccggacgtag
    18961 gtacttccac gaacttaaac cctgccatgg atggaaaaat tttcggtgcc cctgtggtgg
    19021 ctgaagatct agaatccgaa ttgggcgtaa aagatcacaa actccccgat ttgattcctg
    19081 gtgaagatgt cagcgatcgc ttcctccgtt ttgaaattgc ggagggcaac gtgatgtctt
    19141 gcgtaggcgc tttcggccgc ctccgtgata ttattggggt tccaatcatt ccgttgatga
    19201 ctgtctatga cttcttcatc aaacgcgctt tggatcaata tttctacaat ctttactgga
    19261 aaagttcttt catttgcgtg ggcacgcctt cgggtgtgac tttgtctcca gaaggagcgc
    19321 aacacggttg gaagtccgat atccagattc caaatcaaat cacttgggag ccgttcttct
    19381 gccaagagtt ggattggatt ctgtgtgacg ccattaaacg tcatgtgatg aacaataatg
    19441 cgggccgttc tggagtcctt ctccgcctcg tcactcgcgg tattgagcaa aaagacatga
    19501 tgtactactt gaaaaaacag gctcgtttca aaacagattt gagtgcaaaa ctggtacgtg
    19561 ctgaattccc agttaacggc ggcaccaatg aagaagaagc cgtgggcatg gacgaaggac
    19621 aaatccttca tcaagttcgt gaagaagttc ttcaaggagc ttactatctg atcgactatc
    19681 gtggatatgc tggctatgag cctggggaca acgtcgtcaa tatttttgcc atgggcgcaa
    19741 tggtaacgga aggaatcaag gcttctgaga ctctgcttgg ccatggcatt tacgccaatg
    19801 tcatcgctgt gacttcgcca gatctcttgg ctggaattca ggctcacgaa aatgactata
    19861 cctatttaaa acacggttta ggaatcaact cagatttata tttaagaaag tcagaagatg
    19921 tttccgccgg agatctgatc accgttgccg gtaaacgcgt tcctatggtc agcgttcatg
    19981 atggagaagc gggtttgttg gataacttgg gatccattat tggcgtccgc catgaggcct
    20041 gtgctgttag aaagcattct aaatgcggtc gtccctctga gatttacgct tttcacggca
    20101 tcgatgcgaa aagcgtcgta gaagcttgtg gtaaagtgtt agcggaaaca gcattggagc
    20161 aagtgatagt acgagatgtt gctttggagc aaactcagca agcggggaaa acgacaagcc
    20221 attggaccga actttggcca acaaaaaacc ccatgaataa acactaggaa tcaaatcaat
    20281 gaccacaaca gtctctcttc cttacaaagg tcggcttttt aaagcgagaa aaaaaaagta
    20341 caacgagact gttttgttcg ttcctttcta tgaggccaca caacgcgagc tggcccgcca
    20401 cgtgaagttc gtctgtgatc ttggttatga tgcggtcctt ttcgatctgc aaaagccccc
    20461 cgagatcctc aatttctccg aaggcattct gacgccgctc aagaaatgga aagaactcct
    20521 catgcctttt gaagtcccga tcaattcgca aatgaagttt ggactgaagc atgtctatgc
    20581 ggatcagata gaaaatatgc tgaatcatat tcctggaaaa aagattctct tcgcattctc
    20641 caatcccggc ggggctgcca ttgaggcatt ggctcgtcgc aaatgcgcgg atgtcacagg
    20701 tgtgatcttt gatagcggac cttcaatgaa attcgttgaa tctttctcga acttcgttca
    20761 ttacaacttg aatatcacac ctctgattct gcgctatact ctggctccta ttttgtccta
    20821 cgcctggagt cccgacctgc ataaagattt gcacacggat ctcgcacagt ttcctcaggg
    20881 gtttaaagtt ctttccattc gtgggtggaa agatcgactg attcctcctg accatatcga
    20941 tgcggttttt gagccccatc ataatttgga ttggcgaaaa ctcagccttc ccgaagcgga
    21001 tcatctctta ggtcttcggg atcaccgcag cgaatatgct cccgtggtaa aggattttct
    21061 tcacgagatc tcaactcagg agcgcacgaa cttcagtaat tcagcgaagt ttcgggcgtc
    21121 cgcttgctgc aaagaatgaa tgtattttgc tctgacttcg ctatgctctt caagggattc
    21181 atgggccttc attttggccc cccaagtgaa tcgcttataa cccaaactga acaaaagcac
    21241 atccgataca agacgggccc aacgcccatt tccattagga aagggatgta tcaaaaccaa
    21301 acgatgatga agtcgcgttg caatctcatc gatgggatag gtttcatgag tgatccaata
    21361 acttacatct ccaataagtt tattcatctc tgttggaata ttataccaat caacaccgat
    21421 agatttatca gaggttctat aggttcctgc ccacttccaa acatgaccga acattcgctt
    21481 gtgcaaatcg cgcatgaatt tttcagtgag aaaatctttt cttttttttg caaaggccca
    21541 cttttctccc tgaagaatat tatcctgttc agccgcattg agttcttttt gtgctgaaat
    21601 ataagatagc ttgaggccag cgatttcgtt tggatttaat ggagtggcgc caggggcata
    21661 ctctaattta aaatcactca tctaaggacc ataacctttt atcaagttta tttttcaact
    21721 ccctagctaa ttcctcctga tgaagagttg tttcggcttc acccgcacct tgcatctcta
    21781 gatccatcgt atgaactgtt cgcttcaaaa tttctctggc ggcctttccc gcctgttcat
    21841 ccacgatttc ttccagactg tccttgggca cgagagcata aaccaactca cagttcatgg
    21901 cgtgggcggc tcgctgcaag gtctcg
//
LOCUS       DATBTE010000356         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2371922, whole genome shotgun sequence.
ACCESSION   DATBTE010000356 DATBTE010000000
VERSION     DATBTE010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2371922"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..630
                     /locus_tag="VIG33_13355"
     CDS             46..630
                     /locus_tag="VIG33_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEY8271872.1"
                     /translation="MILDQEFFFQKTESVAKQVLGKVLCFENRNGQILSGRIVETEAY
                     LGICDPACHSFGNRKTERTKTLYLPGGYSYIYLIYGMYYCLNFVTREENIPEAVLIRA
                     LQPVEISEGNLSKKNLLTNGPGKLCRYLGITSKENAIPLWRKNSRLWVEDDGFAMKPR
                     SIITTVRVGIDYAKEAAQWPLRFYLKGNLWVSRP"
     gene            complement(665..740)
                     /locus_tag="VIG33_13360"
     tRNA            complement(665..740)
                     /locus_tag="VIG33_13360"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(705..707),aa:Phe,seq:gaa)
     gene            complement(790..1959)
                     /locus_tag="VIG33_13365"
     CDS             complement(790..1959)
                     /locus_tag="VIG33_13365"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEY8271873.1"
                     /translation="MKKDMKELGFSTRAIHAGQQPDPTTGAIMTPIYMTSTYVQASPG
                     VHKGWEYSRTHNPTRKAYEDCLANLEGGKYGFAFASGCAAATTIMHLLSHGDHVIAGD
                     DMYGGTFRLFNRVLKNNGMDFSYVDLTNPENFTKALKPNTKLVWLETPTNPTLKLVDI
                     RKIAKAAAEKGILTVVDNTFMSPYFQKPLLLGADIVVHSATKYIGGHSDVVGGVAATN
                     RPDLAEKIAFLSNSMGGVQGAMDSFMCLRSLKTLPLRMKAHEFNAKAIAEFLEAHPKV
                     EKVIYPGLKSHPQHALAKEQMTGMGGMITFYVKGGLEAARTFLESVQIFALAESLGGV
                     ESLIEHPAIMTHASVPAENRKALGIDDSLIRLSVGIEDLEDLLGDLKSAFDKVKG"
     gene            complement(1970..>2351)
                     /locus_tag="VIG33_13370"
     CDS             complement(1970..>2351)
                     /locus_tag="VIG33_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEY8271874.1"
                     /translation="ASYKVEGVGEDMLPDNVHLNMYDGFIKVSDQEAFDLTRELVAKE
                     GLLVGPSSGLALMGALKASEKMEKSSNIVVIFPDSGRAYLSKAFNNQWMVENGLRKAE
                     EIKNAFGRVILAEQALADLQKNKE"
BASE COUNT          652 a          559 c          467 g          673 t
ORIGIN      
        1 tatctcagga gtgactcctt cgaggttttt atgtaaatta aattgatgat cttagaccag
       61 gaattcttct ttcagaagac tgaaagcgtc gccaagcaag tattgggcaa agtgctttgt
      121 tttgagaatc gaaatggtca aattctctcc ggcaggattg tcgaaactga agcctatttg
      181 ggaatttgcg accctgcttg ccactcattt gggaatagaa agacagagcg cactaaaact
      241 ttgtatcttc ccggcgggta ctcctatatc tacctgatat atggaatgta ttactgttta
      301 aatttcgtca ctcgcgaaga gaatatcccc gaagcagtct tgattcgagc tcttcagcca
      361 gtggagattt ctgaaggaaa tctttcaaaa aaaaatctac taaccaatgg cccaggaaag
      421 ctctgccgct accttgggat taccagtaag gaaaatgcaa ttcctctctg gcgaaaaaac
      481 tctagacttt gggtcgaaga cgacggtttc gccatgaagc caagatcaat tattaccaca
      541 gttcgtgttg gaatagacta tgcgaaggaa gcagcccaat ggcctttgcg tttttatctt
      601 aaaggcaacc tttgggtttc tcgtccctaa tatcgaatcc ttatattaat tttgaagaaa
      661 taaatggtgg ctcgggacgg aattgaaccg ccgacacaag gattttcaat cctctgctct
      721 accgactgag ctaccgagcc atttgaaatt caggaaggta cattagttac cttcctgata
      781 ggctgagtgt caacctttca ccttatcaaa agctgatttt aaatcaccca aaaggtcctc
      841 aagatcttcg atccccactg aaagccgaat taaagaatca tcaattccca aagcttttcg
      901 attttcagct ggcacggagg catgcgtcat gatggccgga tgctcgatca aagactccac
      961 ccctcctaag ctttctgcga gtgcaaaaat ttgcacactt tccaggaaag tccgtgcggc
     1021 ctcaaggcca cctttcacat aaaaagtgat cataccaccc atccccgtca tctgctcttt
     1081 tgctaatgca tgctgaggat gggatttaag tcctggataa atgacttttt ccacttttgg
     1141 atgagcttct aaaaactccg caatggcttt tgcattgaac tcatgagctt tcatacgaag
     1201 aggaagggtt ttcaggcttc tcaagcacat aaaagagtcc atagcccctt gcactcctcc
     1261 cattgagttg ctcaagaagg ctattttttc agcgaggtcc ggacgattcg tagcagccac
     1321 gccgccaaca acatcactgt ggcctccaat gtattttgta gccgaatgaa ccacgatgtc
     1381 agcacctagt agtaaaggct tttggaaata gggactcata aaggtattat ccactaccgt
     1441 gagaatgcct ttttccgccg cagcttttgc aatctttcta atatcaacca gtttcaaagt
     1501 aggattggtt ggagtttcta accagaccaa tttggtatta ggtttcaagg ccttagtaaa
     1561 attctctgga ttggtaagat ccacataact gaagtccata ccgttatttt tcaaaactct
     1621 attaaataag cgaaaagttc cgccatacat atcatcgccc gcaataacat ggtccccatg
     1681 actcaacaaa tgcatgattg ttgttgcggc agcacatcct gaggcaaacg caaatccata
     1741 ttttccacct tcaaggttcg ctaaacaatc ctcataagcc ttgcgggttg gattgtgcgt
     1801 gcgactgtat tcccaaccct tgtgcacacc tggagatgct tgcacataag tagatgtcat
     1861 ataaattggg gtcataattg cgccagtcgt tggatcaggc tgctgccccg catgaatcgc
     1921 acgagtggaa aaaccaagtt ccttcatatc ttttttcata acggcatcct tattctttat
     1981 ttttttgcaa atctgcaaga gcttgttctg ctagaatcac gcgaccaaag gcatttttaa
     2041 tctcttcggc tttacgtaaa ccattttcga ccatccattg attattgaat gccttactga
     2101 gataggctcg accgctgtct gggaaaatca ccactatatt tgaggatttt tccatttttt
     2161 ctgaggcttt taaagcaccc ataagtgcca agccacttga aggtcccact aaaagacctt
     2221 ctttcgccac taactcacga gtcaaatcga aagcctcctg atcagagacc ttaatgaagc
     2281 catcatacat atttaggtgc acgttgtccg ggagcatgtc ctctcctact ccctccactt
     2341 tataggaagc c
//
LOCUS       DATBTE010000357        22113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1551162, whole genome shotgun sequence.
ACCESSION   DATBTE010000357 DATBTE010000000
VERSION     DATBTE010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 22113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22113
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551162"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="VIG33_13375"
     CDS             <1..1316
                     /locus_tag="VIG33_13375"
                     /inference="COORDINATES: protein motif:HMM:NF014147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HEY8271875.1"
                     /translation="EGIQTKKYQDFTSGTPSENPTIVKIDTAILHQSMIGFGGNITES
                     CLENLKKLSPSDKENFFKIFFDQKSGAGFSYLRVPIGANDFSIGDYSLNDTPGNKPDP
                     QLKLYNPKKLQVFIQFIQEAKKYNPDIKLIMTPWTAPAWMKDTKSLKGGQIKKKYYRA
                     YSDYLIKTINEFESKGIKIDQMTILNEPLIGYAKEHWDFPQTYMSPQDQSHFIQDSLL
                     PKLKGKQTKLLIHDHNWDNAHAADPVFADNKQNPAVQGIAYHCYGGSLSDLQKNLKNH
                     PGVPAFNTECTSSLNHHSDEDTFNWWQDTQSLDAIKSGISGSLGWNLCLDEKGGPQNN
                     GCEDCRGLMTIDSKTSAMTLNPEFKALATTSRVLDSGSIMIDSKESNPQATSIAFKNP
                     DGSLSMVLKNKSKISQAYNVNVDSCASKKYFLPAGATLSVRWFPD"
     gene            complement(1319..3049)
                     /gene="ptsP"
                     /locus_tag="VIG33_13380"
     CDS             complement(1319..3049)
                     /gene="ptsP"
                     /locus_tag="VIG33_13380"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEY8271876.1"
                     /translation="MKAIAVSPGYAHGKVFILQDEVNWNNISLQTNDVPGEVSKFNLA
                     IQKSAAEIKELILQFKGSNQKDQADIFEAHALMIEDPEVLEQTIGLVQTQAMSAAWAY
                     KKTSDGYAQMLAALDDEYLKERAADVRDIAGRVLKHLLGKAQENLKELPLGSILVARD
                     ITPSQISLLDAKQVGVSVMGIITEMGGKTSHTAIIARALEIPSVAGVQGILEQVENGT
                     EILFDGVTGQIKIQPTLEDIKDFNFKKNQFEAQKKDLLSFRRLKSETHDKRTVRLAAN
                     IGGVSDVFSLISNDAEAVGLYRTEFVFLDRNRVPSQEEQFQIYREVFEKLGERHCIIR
                     TLDIGGDKQLEGLSMDQELNPFLGLRGVRLCLREQNIFKDQLKAILRAAVGHSVGIMV
                     PMISNIEEILETKKIIKECQSELRACGQKFSEGFKFGVMIEVPSAAMIVDFISRHVDF
                     ISIGTNDLTQYICAVDRLNDKVEKLYNPYNPGFLRTMNSILSAARKNNLHVGICGSLA
                     HEEFLVPLFIGMGVDELSMTSQHILQTRKLVRSLNYQDCQKLVEEVLQLETSAEIKAK
                     IQAGKSKEKA"
     gene            complement(3056..3316)
                     /locus_tag="VIG33_13385"
     CDS             complement(3056..3316)
                     /locus_tag="VIG33_13385"
                     /inference="COORDINATES: protein motif:HMM:NF012599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY8271877.1"
                     /translation="MEIKLRLLNEEGMHARPAGILVKAISSFQSKVELEFNGKSINAK
                     SLLSILSLGLTFNSEFMARAEGSDAALALKSIESLIQNKFQV"
     gene            complement(3347..5248)
                     /locus_tag="VIG33_13390"
     CDS             complement(3347..5248)
                     /locus_tag="VIG33_13390"
                     /inference="COORDINATES: protein motif:HMM:NF014437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HEY8271878.1"
                     /translation="MMKIKEFLNRFFPTLQKIGQSLMVPVSVLPAAGLLVALGRILKD
                     LDKNNSFIGQVLFSGGLAIFEQLPLIFAIGVAIGFTGGAGIAGLAAAVGYFTMTNILK
                     IVSETEKLNLAINTGVFGGIIIGLLAAQICNRFQNVKLPQVLGFFSGKRFVPILMTAV
                     SFILALILGAIWPPIQKGIYEFGNSVINSDYGPAFYAAGKRLLIPVGLHHVYYPPFLY
                     EFGQFVTSAGQVIKGESARYFAGDPTAGRIMASEFPLMLFGLPAAALAMFLAAPKGKR
                     NVVGGVMLSAALTSILTGITEPIEFAFIFVAPLLYVLHVCFAFLAGVLTAHFDIHLGY
                     TFSASLIDYALGYFNQKNSSFLFLAVGPVISLLYFSSFYSLIHFFKFQTPGREVEKSG
                     EETSVAVTKNTAKSAIAAEVLKALGGKQNIESLDACITRLRVVVRDASKVQVDSLKKL
                     GAAGVMESGKSIQAIFGVESDFLKEAIKISMLQSHLLSPAKGKILKLSGVPDKTFAEG
                     ILGDGFAIEPEEGVIFSPCSGQIVNVFPTKHALGILSDEGLEVLIHVGIDTVNMKGEG
                     FKCFVNEGDHVNAGDKLIEFDLALVRQKATSLITPIVITNMDTVKSLKLLHEGATPAK
                     QRVLEALFR"
     gene            complement(5420..6745)
                     /gene="scpB"
                     /locus_tag="VIG33_13395"
     CDS             complement(5420..6745)
                     /gene="scpB"
                     /locus_tag="VIG33_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY8271879.1"
                     /translation="MAKKKNVSDLAQEAFVSASLFDEEMSVPVSAAEKNSDGDSLPEE
                     FFSDIETETSIFLQEEEESVGFLPEVNEESATAASEVSLEGTELDSFESAEIEELEFV
                     EEERLESIVESILFASDRPVSLASLKLVFKGTNINTQKLRRTLDNLAVEYAGGQRGVT
                     LEEVLGGYQLRTKIDNMDFMRRTLKARPFKLSGPALEVLAIVAYKQPLVKSEVDVIRG
                     VESGHLLRALMEKNLVCFEGKSELPGRPMQYGTTRKFLEIFGLRNLKELPTLSQIDEL
                     LPEGIGEEDEKEKQTLSTITDSMASMVGSSYSQGEDELMKITEQLEEITTSSDFFEQE
                     KQRQREKRDQEKAQNIREALAMDEQVSNRDINWLKRYDEALAAEQGAPAELASNQEVE
                     QSAAADVENAELQRAIAAFDAEEDEEDETEDELPFLGEADDIDEDDTKF"
     gene            complement(6738..7760)
                     /locus_tag="VIG33_13400"
     CDS             complement(6738..7760)
                     /locus_tag="VIG33_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEY8271880.1"
                     /translation="MSINIQLPKFEGPLGLLLYLIRKEEMDIMDINIQEITKQYFEYI
                     KLMKELDLEIAGEFVAMASTLIHIKSRMLLPQYDENGEIVENEDPRKELVQKLLEYQK
                     YQEAAKLLYERPLVGRDLWLRGSREVLAPKEDEIILEENALFSLIGAFRKVVKTAKKK
                     IHQVAAKAQSISSRILEIKDRLVVGQKVIMRDLITESEDRARQTLITFLSLLELGKMG
                     FVSLFQTETYSDIYIEAKKIVEGDVLSSVEEYGSVNSELMAEQMIAKSEKEAAEAGEA
                     SAQNEDFVLKDAEDNEQMLLTPLPVDQFLAGTEDMASDDDIFAAEKELFTNDINDGNL
                     EGGALG"
     gene            complement(7764..8786)
                     /gene="trpS"
                     /locus_tag="VIG33_13405"
     CDS             complement(7764..8786)
                     /gene="trpS"
                     /locus_tag="VIG33_13405"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY8271881.1"
                     /translation="MSGENPSLQKPPRRQRVMSGIRVTGPLHIGNYWGALSNFVKLQN
                     EGLYECFFGAMDWHGMTTAYRSTQDVVPWTREMIAEFIAWGLDPEKSTIFVQSRVPEH
                     IELFMILANLTPMGWLERVTTWKDAEEEAKQNDTHNLGRFAYPVLQTADILIYRGEKV
                     PIGKDQTAHLEISREIIRRFNNIYKAKLPEPTPLFTEVPVLPGLDGRKMSKSYGNTLY
                     LTEDEKSLKKKVNLMVTDPQRGRREDVGNPEVCSVYGYHKLYSSAEDLNWATQGCVTA
                     GIGCGDCKAKLFGNIDTLTKEPREKKKELLNNPKLLDSILDAGCEKARKEAQKTLGIV
                     RAQMKW"
     gene            complement(8783..9442)
                     /locus_tag="VIG33_13410"
     CDS             complement(8783..9442)
                     /locus_tag="VIG33_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEY8271882.1"
                     /translation="MDLMELGAKVGAYYIPFLFALCFHEFAHGWVAKLRGDNTAQMMG
                     RLTMNPLSHMDMIGTFLLPLFSIAFNTPIFFGWAKPVPVNARNLKNPRKDMFWVALAG
                     PLSNLLLALIGALIIGITARYLLVSEVVMSFLKIVKIFITTNLFLAVFNLIPLNPLDG
                     GKVLARFLPAHLNYKLEQNEHITSMILMVLILTGALHVLSIPVFWGQELLMKTALGGL
                     L"
     gene            9599..10780
                     /locus_tag="VIG33_13415"
     CDS             9599..10780
                     /locus_tag="VIG33_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY8271883.1"
                     /translation="MMSISVDARPDARPALTTLSEDEKAFQEAVRAFAETEIKPHVTR
                     MDEKAEMDPAIVKKLFEMGLMGIETPEKFGGAGSTFTMACLAVEELGRIDGSVSVLLD
                     VQNTLVTNAFLKWGSETLQAKYLSKMAAEWVGAYALSESSSGSDAFALKLKAELKGDK
                     YVLNGSKLWITNGKEANVFIVFANVDFGKGYKGITAFIVEKTFKGFTVGKKEDKLGIR
                     ASSTCELLFENCEVPVENVLGEVGKGYKIAIETLNEGRIGIGAQMVGIAQGAYEATLN
                     YIKSREQFGKSIAQFQGVQFQLAEMRTELEAARLMVYNAARLKDAGQDFIEAAAMAKL
                     FSSRAACNITSTAVDLFGGNGFTKEYPVEKFWRDAKIGQIYEGTTNMQLQTIAKMELD
                     K"
     gene            10791..12350
                     /locus_tag="VIG33_13420"
     CDS             10791..12350
                     /locus_tag="VIG33_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8271884.1"
                     /translation="MNYIRIIYRLICLGLFLDFLIFVPTAKAGEEIPAPVFKIHLSSE
                     PSSLDPHLQKSSASSYLLQNLYRNIFFYDNDKGLKPDLGESCKRETPFVLVCKLKKGL
                     QWSDGSRLTSEDFLKAYQRILTPQEASPRADLLFKIKNAPAIYKGSKKMETLGITAPD
                     ATTLRFEFEEKDPDFEYNLASFLLSPIKNSLKPGQKASELIVNGPYKISDWIAGQKIQ
                     LINNSLYKNGNFAKRPLIYFFFINEDSMALQLYEKNELSFLRRLPSLYIPKFKSRPDF
                     HWIPMIRLDYFGFGPLLKDHPKLREALSLSLNYKELQKIFSSQGLPGCPGVPSSWLIE
                     ELCYTQDIAKAKKAFSEDKAVPSNLKLLFSSLGGDDHRRATEWMQAQWQKNLDLKIQL
                     LLRENKVFLHELEKSPPAIFRKGVAPDRPTCLATLETFADWSPENYIQLKDPEYQNVL
                     KKLGEAVTPQDQKKFCTQGVRFLMDHHLFIPSGPMHFSILAKRNFKGWKLNSMNQLDL
                     AELSSSQADTK"
     gene            12488..12724
                     /locus_tag="VIG33_13425"
     CDS             12488..12724
                     /locus_tag="VIG33_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271885.1"
                     /translation="MAENNSSHKIEKQIPHNVVQLGPSKCCGEGCNKKPEKAGFCGEH
                     FEWFKAGLLTKDGHKAADFEKKHYHFMKAKKSAA"
     gene            12997..13743
                     /locus_tag="VIG33_13430"
     CDS             12997..13743
                     /locus_tag="VIG33_13430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY8271886.1"
                     /translation="MKKAVLITGASSGIGQATALEFSRQGYFIYLMGRNKERLEETAL
                     RCCAGACLLSCDLKYPAQVEKRLNEIYENPLYRLEILINNAGVFKTHSMEEGSDEIWT
                     EQFETNLLGPVRLTRLLIPYFKKYQKGSIVNISSTLGLRPSSNTGAYSALKAALINWT
                     QSLAQEAGAYGVRVNCICPGIVDTPIHSFNNLPESQKQKVLEEMAKLQPLGRIGTPED
                     LAKSVYFIGSELSAWTTGAVLSVDGGIYLQ"
     gene            13740..14123
                     /locus_tag="VIG33_13435"
     CDS             13740..14123
                     /locus_tag="VIG33_13435"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEY8271887.1"
                     /translation="MTKTFIVTSSFSAAHFYRQKKWSSLENQICFGKCFTEYGHGHNY
                     RLEVGFQTPQALLLDLQQLVKAITDPLDHQHLNFVIAEFKDLVPTTENIAKYLLKKLH
                     EGCPNEFISYIKLYEMDDLWVEIRI"
     gene            14120..14884
                     /locus_tag="VIG33_13440"
     CDS             14120..14884
                     /locus_tag="VIG33_13440"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEY8271888.1"
                     /translation="MSNDENKGKGEGKDKVRLSKLMAEKGLCSRREADIYIAEGLVYV
                     NGEKVSVLGTKVSPQDRVTLDARVLKTQNNLATILLNKPVGYVSAQPEPQYTPAIKLL
                     TPENQFGVSKQKLKSDSFAGLAVAGRLDIDSQGLLIFTQDGRIAKQIIGENSKIDKEY
                     LVRVEGILPKEKLQLLRYGLELDGKKLKPAVVEWVNEDQLRFILNEGKKRQIRRMCEL
                     VGLRVRGLKRVRVGKITLGQLPEGKWRFLEADESLD"
     gene            14944..16575
                     /locus_tag="VIG33_13445"
     CDS             14944..16575
                     /locus_tag="VIG33_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8271889.1"
                     /translation="MLINIRFNIGSVISSVLGSIFLSGLISLPVAQAKPEGKILVYCS
                     EGNPSSFNPQLVSDGTSLNSTHPVYNGLVEFELGSTKVIPMLAESFTVSKDKLTYTFK
                     LRKGVRFHTTKNFTPKRDFQAEDVLFSFERQWKKNHPYHNVGGATYEYFEGMDMGKVI
                     KSIKALTPMTVEITLNHPEAPFIANLAMPFMNILSKEYADQLTAQNKQADIDLLPVGT
                     GPFVFQSFIKGSSIKYTSFENYWGKKGNIEKLIFAITPDANVRTQKLKTGECQFATEP
                     APTDLASLKADVKISVLNAPGLNVGYLAMNVTKKPFDNLLVRKALNYALNRKAYIEAI
                     FLGNAIVAKNPLPPTIWSYNESVKDYDYNPEKAKELLKQAGFPNGFETEMWTMPVSRP
                     YNPNGKKMGEMMQADLVKVGIKVKLITFDWPTYLAKSKTGEHTLIQFGWTGDNGDPDN
                     FLNVLLGCSSVEAGSNFSRWCHQPFNDLILQAKANPDIKKRTELYKKAQVIFKEEAPW
                     VTLDHSTVFRAMSKNVKNYKMDPLGADIFNNVNIE"
     gene            16588..17619
                     /locus_tag="VIG33_13450"
     CDS             16588..17619
                     /locus_tag="VIG33_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8271890.1"
                     /translation="MFLFLSKKILEVSFTLVGITFICFLLLRLVPGDPVLLMIGERNM
                     DPVAYAEAKQKLGLDKPVLVQFGKYIEQVASGDLGTSVTSKTSVWTEFKERFPATVEL
                     SFVALLFATLFAIPFGVLAALKKNTFFDYFLMGSSLTGYSMPIFWWGLVLILLFSVKL
                     GWTPVSGRIAVEYDIPLKTGFYLLDSLQRDVREAEGLKPFWSAVSHLILPSIVLGTIP
                     LAVMARMTRSSFLEVLGEDYIRTARAKGLSEYRVVLIHSFRNALIPIVTTLALCFGSI
                     ITGAILTETIFSWPGIGRWIVTSITSRDYPTIQGGVLIISVIIIITNTLVDLLYIYLN
                     PRIKNALLK"
     gene            17623..18486
                     /locus_tag="VIG33_13455"
     CDS             17623..18486
                     /locus_tag="VIG33_13455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8271891.1"
                     /translation="MKDFFSELLKDIKKNKGAFAGLLLILIFILLAIFAPLLSVYAPD
                     QVIEGALRLPPNSHFQNYYFIFGTDDIGRDLFTRLLYGSRISLLVGLSVVVLAASLGT
                     SLGLISGYFGGWTDRIISRAMDFIMALPSVLFAIVIVAVLGPGLMNAIVAVSIVALPN
                     FVRLIRAQVLAEKNKQYVQAAQLYGASHLRILFKEILPNCMAPLIVQGSLGFSDGILN
                     CAALGFLGLGAQAPTPEWGTMLSDARSFLESSPWMVNLPGLCILTVVLSFNLLGDGLR
                     DALDPRLKNRS"
     gene            18499..19479
                     /locus_tag="VIG33_13460"
     CDS             18499..19479
                     /locus_tag="VIG33_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8271892.1"
                     /translation="MHLNNKLLEIEKLSVQFKTDSGAIEALRPISFTVLEGEILGVVG
                     ESGSGKSVTNLALMGLLPANAQITAQKVVFNKNPLNLNSQKDLRAIRGSQIAMIFQDP
                     MTALNPSLTVGYQMIETLRCHFDISKKVAEHRSLALLKRVGISNPEARLKSYPFELSG
                     GMSQRVMIAMAISCHPKLLIADEPTTALDVTIQKQILDLLLDLQKENKMSMIFVSHDL
                     ALIKKYAHRVQVMYAGEIVEGGNTEEVIKTPRHPYTFGLLKARPSAHEGKMKEPLYAI
                     PGVVPSLRERPSGCQFHPRCFRATELCKKQAPLLTTAARNFACHHPLEIL"
     gene            19476..20240
                     /locus_tag="VIG33_13465"
     CDS             19476..20240
                     /locus_tag="VIG33_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY8271893.1"
                     /translation="MSLLKVKNLKKSYVTKNLFFGAKKEEPVLKGISFELEENQILGV
                     VGESGCGKSTLAKVLTRLEEASGGEIIFNGKDLRSFSSKEFPHQIQMVFQDPFQSLNP
                     RKKAWEIIADPILVTENISTSEAKQRALAMMQKVGLLTETSERYPHQFSGGQRQRLGI
                     ARALMLRPKLLILDEPVSALDVSIQAQVLNLLLDLQKEFHLSMIFISHDLSVVKHISD
                     KTLVLYFGEIVEMGPSTQIFSEPQHQYTQKLLESAP"
     gene            complement(20269..20697)
                     /locus_tag="VIG33_13470"
     CDS             complement(20269..20697)
                     /locus_tag="VIG33_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271894.1"
                     /translation="MTVFKGTFLGFFSFCSLISFSTTSFAVSGLTTMHQENCKSYPPG
                     TSRNLPPQFCFKFVGVPVWSEAKREIKTLNAMDAKAAAYCAELNKVAYRVYSETGTNA
                     VEMLPVGTSIEGGYVCLTQAQGAEAEKIYRVEGEQLGASN"
     gene            complement(20775..21479)
                     /locus_tag="VIG33_13475"
     CDS             complement(20775..21479)
                     /locus_tag="VIG33_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271895.1"
                     /translation="MKNEKVPRNLRHFVKFLDTTFASKLFYLWLDIGTDIASGISQRN
                     RAPANWEVNPTGFGTVSRSLSMKKITAIVLSMVCASIAQAKIEGKYSLKSSDLSEGSS
                     LCSSTLVISKLATDTLKVEGARDLGSYYNLDGAEGAVIISGINRGKQVSESKNSSHGE
                     KYSTTNLAAQEGDVLKLTSNSKTKAFGVTLDESLNELMIEVKSNTLMLSLKRSHKVRF
                     DGQGNWSAEENCTYSK"
BASE COUNT         6245 a         5192 c         4546 g         6130 t
ORIGIN      
        1 cggaaggaat ccaaacaaaa aaataccaag attttacctc aggaacccct tctgaaaacc
       61 ctaccatcgt taaaatcgac acggcgatcc ttcatcaatc tatgattgga tttggcggta
      121 atatcaccga gtcctgcttg gaaaacctta agaaactttc cccctctgat aaagaaaatt
      181 tctttaaaat tttttttgat caaaaaagtg gagcgggctt cagctatctc agggttccta
      241 ttggtgcaaa tgacttctcc ataggcgact acagcttgaa cgatacgccg ggaaataagc
      301 cggacccaca acttaaatta tataatccta aaaaactcca agtattcatt caatttattc
      361 aagaagctaa aaaatataat cctgatatta aattaataat gactccttgg acggcaccag
      421 catggatgaa agacaccaaa tccctcaagg gtggtcaaat taagaaaaaa tactaccgag
      481 cctattctga ctatttgata aaaacaatta atgagtttga gtctaaagga atcaaaatag
      541 atcaaatgac gattttgaac gagcctttaa tcggatatgc gaaagaacat tgggattttc
      601 ctcagaccta tatgagtcct caggatcaga gtcattttat ccaagacagt cttttaccta
      661 aactcaaagg aaaacaaacg aaactactca ttcatgatca taactgggac aacgctcacg
      721 ctgccgaccc cgtttttgct gacaataaac aaaatccagc agttcaagga atagcttatc
      781 actgctacgg cggaagtctt tcagatcttc aaaaaaatct taaaaatcat ccaggagtcc
      841 ctgcctttaa tactgaatgc acgtcttctt taaatcacca ttccgatgag gatactttta
      901 actggtggca agacacgcaa agtcttgatg ctattaaatc cggaatttcc ggatctcttg
      961 gatggaatct ttgcctcgat gaaaaaggtg ggcctcaaaa taatggctgt gaggattgtc
     1021 gcggattgat gacgattgat tctaagacaa gtgcgatgac cctcaaccca gagtttaaag
     1081 cgctggccac aaccagtaga gttcttgatt ctgggtcaat catgattgat tcaaaagaat
     1141 ctaatccgca agcgacaagc attgccttta agaatcccga tggcagtctc tcgatggttt
     1201 taaaaaacaa atccaaaata tctcaagcct acaacgtaaa cgttgatagc tgtgcttcta
     1261 aaaaatactt cttaccagcg ggagcgacac tctctgtgcg ctggtttccg gattagactc
     1321 acgccttttc tttagatttg cctgcttgga ttttagcttt aatttccgcg ctcgtttcaa
     1381 gctgcaaaac ctcttcgaca agcttctggc agtcttgata gttaaggctc cggaccagct
     1441 tgcgggtttg caaaatgtgt tgtgaggtca tactgagttc atcgacaccc attccaataa
     1501 ataaagggac aaggaattct tcgtgagcga gagacccgca aattcccaca tgcagattat
     1561 ttttacgagc tgcacttaaa atagaattca tagtccgcaa aaaaccagga ttgtaggggt
     1621 tatataattt ttcaacttta tcgttgaggc gatccacggc acagatatat tgtgtaagat
     1681 cattggtacc aatgctgatg aaatcgacat ggcggctaat gaagtcgaca atcatggcgg
     1741 cactcggcac ttcaatcata acgccaaact taaagccttc tgagaatttt tgaccacaag
     1801 cccgcagctc tgactgacat tctttaataa ttttttttgt ctctagaatc tcttcaatat
     1861 tggatatcat cggaaccata atgcccaccg agtgccctac cgctgcacgc agaatggcct
     1921 tgagctggtc cttgaaaatg ttttgttcac gtaaacacag tcggacccct ctcaatccta
     1981 aaaaaggatt gagttcttga tccatcgaaa gtccttccag ttgtttatct ccaccgatat
     2041 caagggtgcg aataatacaa tggcgctctc ccagtttttc aaaaacttct ctatagattt
     2101 gaaattgttc ctcttgagat gggacacgat tgcgatccag aaacacaaat tcggttcgat
     2161 aaagccccac cgcctccgca tcatttgaaa taagagaaaa aacatcagaa accccaccaa
     2221 tattagcggc aagccgcacc gttcttttgt catgggtttc tgattttaat cgcctaaaac
     2281 tcagtaaatc ttttttctga gcttcaaact ggtttttttt gaaattaaaa tctttaatgt
     2341 cttcaagggt gggttgaatt ttaatctggc ccgtaactcc atcaaataaa atctcggtgc
     2401 cattctcaac ttgctcaaga atcccctgga ctcccgcaac cgaaggaatt tccagggcgc
     2461 gagcgatgat ggcagtatgc gaggtcttac cgcccatttc ggtgataata cccatcacac
     2521 tgacccccac ctgcttggca tccaatagcg aaatctgcga aggagtgata tccctggcca
     2581 ccagaatgct gccaaggggc agctccttca gattctcttg agccttgcct aaaagatgct
     2641 ttaggacccg tccagcgatg tcccgaacat ccgccgcgcg ttccttgaga tattcatcat
     2701 caagggccgc cagcatttgg gcatagccgt cactggtttt tttataagcc caagccgctg
     2761 acatcgcttg ggtttgaaca agaccgatcg tctgctctaa gacctcggga tcttcaatca
     2821 tcagggcatg ggcttcaaaa atgtcggcct gatctttttg attggatcct ttaaattgca
     2881 aaatcagttc tttaatctct gcagccgact tctgaattgc caagttgaat ttgctgactt
     2941 ccccaggcac atcgtttgtt tgcaaagaaa tgttattcca attcacttca tcttgcagaa
     3001 taaaaacttt gccatgggcg taacctggag aaaccgcgat cgctttcata taaacctata
     3061 cttgaaattt attttggatc aaactttcaa tactctttaa ggccagagcg gcatcacttc
     3121 cctcggcacg tgccataaac tcagagttga aagtcaaacc tagcgacagg atgctcaaga
     3181 gggattttgc attgatactt ttgccgttaa attcaagctc aacttttgat tgaaaagagg
     3241 aaatagcctt gactaaaatc ccagcgggac gcgcatgcat gccttcttca tttaacaacc
     3301 tcaatttaat ttccaaaaaa tttctcctgt taagttccaa ctacaattat ctaaataaag
     3361 cttctaacac ccgttgcttt gctggagttg caccctcatg aagcagctta agacttttca
     3421 cagtgtccat attggtgatg acaataggag taataagaga ggttgccttt tgcctcacca
     3481 gtgctaaatc gaattctatc agcttatcgc cggcattcac atgatcgcct tcattgacaa
     3541 agcatttaaa accttcgccc ttcatattga ctgtatcaat tcccacgtga atgagcactt
     3601 ccaaaccctc atcagacaaa attccgaggg cgtgtttagt ggggaataca ttgacaattt
     3661 gaccagagca aggagaaaaa atcactccct cctcgggctc gatcgcaaag ccatcaccta
     3721 aaatcccctc agcaaaggtt ttgtctggaa ccccactgag tttaaggatt tttcccttag
     3781 ccggcgaaag caaatgactc tgaagcatgg aaattttaat agcttctttt aaaaaatctg
     3841 actccacccc aaaaatagct tggatacttt ttccggattc cataactcca gccgcaccta
     3901 attttttaag actgtccacc tgaactttag aagcatccct gaccacgacg cgcagtctgg
     3961 taatacaggc atccagactt tcaatgtttt gcttgccccc cagggcttta agaacttctg
     4021 cggcgatggc ggattttgca gtatttttgg taacagccac ggaagtctct tccccgcttt
     4081 tttcaacttc tcgtccagga gtctgaaact taaaaaaatg gatcagactg taaaaactcg
     4141 aaaaatataa aagagaaatg accgggccca ctgccagaaa taaaaacgaa ctgttttttt
     4201 ggttaaagta acccagtgca tagtcaatca gggaggctga aaacgtgtag cccaagtgaa
     4261 tgtcaaaatg ggccgttaag actcccgcca aaaaggcaaa acaaacatgc aaaacataaa
     4321 gaagaggcgc cacgaaaata aaagcgaatt cgatgggctc ggtaatccct gtcagaatgg
     4381 atgtgagcgc tgcggacaac atcactccgc cgacgacatt gcgcttacct ttaggggccg
     4441 caagaaacat cgccaaggct gctgccggca aaccgaaaag catcaaggga aactccgagg
     4501 ccataattct ccctgccgtg ggatcgcctg caaaatacct cgcggactct cccttgatca
     4561 cctgccccgc gctcgtgaca aattgtccga actcatagag aaaaggaggg taataaacat
     4621 gatgcaaacc cactggaatc agcaagcgct tgccagcggc atagaaagcc gggccatagt
     4681 cggaattaat cacagaattg ccgaactcat aaatcccctt ttgaatgggg ggccaaatgg
     4741 ctccaagtat cagagcaaga ataaaactga ccgccgtcat aaggatagga acaaatcttt
     4801 ttccagaaaa aaaacctaat acttgaggca atttcacatt ctgaaaacga ttgcagattt
     4861 gcgcagcgag cagtccgata atgatcccgc cgaaaacgcc cgtattaatc gcaaggttta
     4921 gtttttcagt ctcgctcacg atttttaaaa tattggtcat ggtaaaataa ccgacggccg
     4981 ctgccagtcc agcaattccc gcgccgcctg taaagccaat ggccacacca atcgcaaaaa
     5041 ttaaaggaag ctgttcgaaa atcgccagcc ccccagagaa caaaacctgc ccaatgaacg
     5101 aattattttt atcaagatct ttaaggatac gacccagtgc cactaaaaga cccgccgcag
     5161 gcaagaccga gaccggcacc atcaacgact ggccgatttt ttgaagggtt gggaaaaatc
     5221 gatttaagaa ttcttttatt ttcatcatgc caatgcctct caaaaaatga attcatctgt
     5281 caaaaacaaa aaacgatttg ccgataactt caagcctctt tgtaaaaaag aacccattgg
     5341 aatgtcgaga tttaatatag ctgccgaaaa tagattcaag ctaataccta aaagagtctt
     5401 taaaaagaaa gacccctttt tagaatttag tatcgtcctc atcgatatca tcagcttcac
     5461 ctaagaaagg gagttcgtct tccgtttcgt cttcttcgtc ctcttccgca tcaaacgctg
     5521 caatggctcg ttgaagttct gcgttctcta catccgccgc agcagactgc tctacctctt
     5581 gattcgaagc tagctctgcg ggtgcgcctt gctctgccgc caaagcttca tcataacgtt
     5641 ttagccaatt gatatcacgg tttgacactt gctcatccat agccaaggcc tctcggatgt
     5701 tttgagcctt ttcttgatcg cgcttttcac gctgacgttg cttctcttgt tcaaagaagt
     5761 ccgaagaggt ggtgatctct tcaagctgct cagttatttt catcaactca tcttcccctt
     5821 gcgagtaaga agagcccacc atggaagcca tggagtctgt gatggtgctg agggtttgct
     5881 tctctttctc gtcttcctcg ccgatccctt ctggcaagag ttcatcgatt tgagaaagtg
     5941 taggaagttc tttgagatta cgcaggccaa aaatttccaa gaatttacga gtggttccgt
     6001 attgcatagg gcgacccggc aactcgctct taccctcgaa gcacacaaga tttttctcca
     6061 tcaaggcccg caataaatgt ccagactcta cgccacggat cacgtccact tcggatttta
     6121 ccaagggttg cttataagcc acaattgcta aaacttcgag agctggacct gagagtttaa
     6181 aagggcgagc tttcaaagtc ctacgcatga aatccatgtt gtcgatcttg gtacggagct
     6241 gatagccacc tagaacttcc tctaaagtga ccccccgctg accgccagcg tattcgaccg
     6301 ccaaattatc caaggtgcgg cgaagctttt gagtgtttat gttggttcct ttaaagacga
     6361 gcttcaaaga agccaagctg acaggacggt ctgaagcaaa gaggatactc tctacaatgg
     6421 actccaaacg ctcttcttct acaaactcta gctcttcaat ctcagcggac tcaaaactat
     6481 caagctcagt gccctctaaa gaaacttctg aagctgctgt ggcagattct tcgttcacct
     6541 ctggcagaaa accaacggat tcttcttctt cttgcaagaa gatcgaagtt tctgtttcga
     6601 tatcagaaaa aaattcctca ggcaatgaat ccccgtcgct gttcttttca gcggcgctta
     6661 cgggaacact catttcctca tcaaataaag atgcagatac aaaagcctct tgcgccaaat
     6721 cggatacatt ctttttctta gccaagagct cccccttcta ggttgccatc attgatgtcg
     6781 tttgtgaaaa gttctttttc cgcagcaaaa atatcatcat cgctggccat atcctcggtt
     6841 ccagccaaaa actgatccac aggaagtgga gtgaggagca tctgctcgtt gtcttccgca
     6901 tctttcaaaa caaaatcttc gttttgagct gatgcttctc cggcttcagc cgcctctttt
     6961 tcagactttg caatcatttg ctcggccatc aattccgaat tcacagaacc atactcttcc
     7021 acacttgaaa gcacgtcgcc ttcaactatt tttttagcct ctatataaat atctgaatag
     7081 gtttcagttt ggaataatga aacaaaaccc atctttccaa gctctaacaa agacaagaaa
     7141 gtgattaaag tctgacgagc acgatcttca gactcggtga taagatctct catgatcact
     7201 ttttgaccca cgaccaggcg atctttaatt tccaaaatac gactggaaat agactgagct
     7261 ttagccgcaa cctgatgaat tttctttttt gccgtcttca caactttgcg aaaagcgcca
     7321 attaatgaaa acaaggcatt ctcttcaaga atgatttcat cctctttagg agccaaaacc
     7381 tcgcggctcc cgcgcagcca caaatcacgt cccactagag ggcgctcata aagtaatttc
     7441 gccgcttcct ggtacttttg atattccaag agtttttgca cgagttcttt gcgtggatct
     7501 tcattttcca cgatctcgcc attttcatcg tactgaggca agagcatgcg ggatttgata
     7561 tgaatcagag ttgaggccat cgcgacgaat tcgccagcga tctccagatc caattctttc
     7621 atcagcttga tatactcaaa atattgcttc gtaatctcct ggatgttgat atccatgata
     7681 tccatttctt ctttacgaat aagataaaga agaagtccca agggaccttc aaatttgggc
     7741 aattgaatat taatactcat taatcaccat ttcatttgag cgcgcacaat ccctaaggtt
     7801 ttctgcgctt ctttacgagc cttttcacaa cctgcatcaa gaattgaatc aagcagtttt
     7861 ggattattca acaattcttt tttcttttct cgaggctctt tagtgagagt atcgatgttg
     7921 ccgaataatt tagctttgca atcaccacat ccgatacccg ccgtcacaca gccttgagtc
     7981 gcccagttta aatcttcagc tgacgaatat aacttatgat atccatacac agaacagacc
     8041 tcagggttgc cgacatcttc gcgacgtcct cgctgagggt ccgtcaccat gaggtttact
     8101 tttttcttga gactcttttc atcttccgtg agatataagg tgttcccata ggatttagac
     8161 attttgcgac catcaagtcc cggcaaaacc ggaacctccg taaaaagagg ggtcggctca
     8221 ggaagcttgg ccttataaat attattgaac cggcgaatga tttctcggga aatttccaaa
     8281 tgcgccgttt gatcttttcc gatgggaact ttttcgccac gataaattaa aatgtccgca
     8341 gtttgtagca ctggatacgc aaagcggccc aagttgtggg tgtcgttctg cttggcctct
     8401 tcttccgcgt ccttccaggt agtgactcgt tcgagccagc ccatgggagt gagattcgcc
     8461 aggatcatga aaagctcaat gtgctcagga accctgcttt gtacaaaaat ggtggacttt
     8521 tctggatcca aaccccaagc gataaactcc gcaatcatct cccgtgtcca aggaaccacg
     8581 tcttgagtgc tgcgataggc agtggtcatt ccatgccaat ccatagcccc aaaaaaacat
     8641 tcgtacagtc cttcattttg tagctttaca aaattactga gtgctcccca gtaatttccg
     8701 atatgcaaag gacctgtcac acgaatccca ctcatcaccc tttgacgacg aggcggtttt
     8761 tgcagcgacg gattttctcc actcataaaa ggccacccag agctgttttc ataagaagtt
     8821 cttggcccca gaaaaccgga attgaaagga catgcagggc gccggtcaag atcaacacca
     8881 tcagaatcat actagtgata tgctcatttt gttcgagttt ataatttaaa tgcgccggca
     8941 gaaagcgagc cagaaccttt ccgccatcca aaggattgag gggaatcaaa ttgaaaactg
     9001 ctaaaaataa attcgtggta ataaagattt ttacaatttt aaggaaagac atgacgactt
     9061 cagagaccaa gaggtaacgc gccgtaatac caataatcaa agccccgatc aaggcaagaa
     9121 gtagattgga taaaggtcct gccaaggcga cccaaaacat atctttccgg ggattcttga
     9181 gatttctggc attcaccgga accggcttcg cccaaccaaa gaaaatgggc gtattgaagg
     9241 caatggaaaa gagcggcaac aaaaaagtcc caatcatatc catatgcgat aaggggttca
     9301 tcgtcagtcg tcccatcatt tgagctgtat tatccccccg caacttcgcc acccaaccat
     9361 gagcaaactc atggaaacaa agagcgaata aaaacggaat ataataagcc cctacttttg
     9421 cacccagttc cattaaatcc atactacaac gctaactgac cactagacag ttgtctagag
     9481 acacttcaac tcctgctgaa agatgaagca gcaaatgacg cgcaacgtgc tcgttgattc
     9541 cagagaagct tgcagctagt ttttagaaac tttttttcat cacaatttga aggagtttat
     9601 gatgtctatt tctgttgatg cacgccctga tgcacgcccc gcactcacca ccctctcaga
     9661 agacgaaaag gcctttcagg aagccgttcg cgccttcgca gaaacagaaa taaaacccca
     9721 tgtgacccgt atggatgaaa aagccgaaat ggatcctgcc attgttaaaa aactttttga
     9781 aatgggattg atgggcattg aaaccccaga aaaatttggc ggcgcgggtt ccacctttac
     9841 catggcttgc ttggcggttg aagaactcgg acgcattgac ggctccgtca gcgtgctgtt
     9901 ggatgtgcaa aataccttgg tcaccaacgc ttttttgaaa tggggcagcg aaacccttca
     9961 agctaagtat ctttctaaaa tggctgcaga atgggtgggg gcttatgccc tttccgaaag
    10021 ctcttcagga tccgatgcct tcgctttgaa actcaaggca gagcttaagg gtgataaata
    10081 cgttctgaat ggttcaaaac tgtggattac caatggcaaa gaagccaatg tcttcatcgt
    10141 gtttgccaac gtggattttg gcaagggcta caaaggaatc accgccttca tcgttgaaaa
    10201 gaccttcaaa ggtttcaccg tgggcaaaaa agaagacaaa ttgggcatcc gcgccagctc
    10261 gacctgcgag ttgctgtttg aaaactgcga agtccccgtc gaaaatgttt taggcgaagt
    10321 cggaaaaggg tataaaattg ctattgagac tttaaatgaa ggtcgtatcg gcatcggcgc
    10381 acaaatggtg gggatcgctc aaggggccta cgaagccact ctgaactaca taaagtcccg
    10441 tgagcaattc ggaaaatcca ttgctcaatt ccaaggggtt caattccagt tggcagaaat
    10501 gcgtacagaa ttagaggcgg ctcgcttgat ggtatacaat gccgcccgcc tgaaagatgc
    10561 gggtcaagat ttcattgaag cggctgccat ggcgaagctc ttttcttctc gagctgcgtg
    10621 caatatcact tccaccgcag tggatttgtt tggcggcaat ggctttacga aagaatatcc
    10681 ggttgaaaaa ttctggcgtg atgcaaagat tggacagatc tatgaaggca ctacgaacat
    10741 gcaacttcaa accatcgcca aaatggagct tgataagtag ataatacgaa atgaactaca
    10801 tccgtattat ttatcgcctt atttgcttag gccttttttt agattttctt atttttgttc
    10861 caaccgccaa ggcgggggaa gaaatccccg cgccggtttt caagatccat ctttctagtg
    10921 aacctagcag tttggatcct catctgcaaa agtcttcagc ctctagttat ttgctacaaa
    10981 atctttatcg caatattttt ttctacgata acgataaggg acttaaacca gatcttggcg
    11041 aaagctgtaa acgtgaaact ccttttgttt tggtttgtaa actcaaaaag ggccttcaat
    11101 ggagcgatgg ttctcgcctc acatctgaag attttttaaa agcctatcag cgaattttaa
    11161 caccgcagga agcttctcct cgagcagacc ttctttttaa aattaaaaat gctcccgcga
    11221 tttataaagg cagcaaaaag atggagactc tggggatcac cgcgccggat gctacgactt
    11281 tgcgctttga attcgaggaa aaagatcctg actttgaata caatcttgcg agttttttat
    11341 tatctcccat caaaaactct ctcaaaccgg gccaaaaagc ttcggagttg attgtgaatg
    11401 gaccttacaa aatctcggac tggattgccg ggcaaaaaat ccaacttatt aataacagcc
    11461 tttataaaaa tggcaatttc gcaaaacgtc cgcttattta tttttttttc atcaatgaag
    11521 acagcatggc cttgcaactc tatgaaaaaa atgaactgag ttttttaagg cgccttccct
    11581 ctctttatat ccctaagttt aaatctcgcc cggattttca ttggattcca atgatacgtt
    11641 tggattactt cggttttggt ccgctcctca aagatcatcc aaaactccgt gaagctttaa
    11701 gtttatctct taattataag gaattgcaga aaattttttc ttcccaaggg cttcctggtt
    11761 gccctggagt gccttcttct tggttaatag aggaattatg ttacacgcaa gatattgcaa
    11821 aagcgaaaaa ggcgtttagc gaagacaaag ccgtccctag caatttaaaa ctcctgtttt
    11881 caagtttggg aggagacgat caccggcgag cgacagagtg gatgcaggct cagtggcaaa
    11941 aaaatctgga tctcaaaatt cagttattat taagggaaaa taaagtcttt ctccatgaac
    12001 tggaaaaaag ccccccagct atttttcgca aaggtgtagc tccagatcgc ccgacctgtt
    12061 tggcgacgtt ggaaactttt gcggattgga gtccggaaaa ttatatccaa ctcaaagacc
    12121 ctgaatatca aaatgttctt aaaaaactcg gcgaggcagt gacgccgcaa gatcaaaaaa
    12181 agttttgcac tcagggagtg cgcttcttga tggatcacca tttattcatt ccctccggcc
    12241 cgatgcattt ttctattcta gctaaaagga atttcaaagg ttggaagctc aactccatga
    12301 atcagttgga cctagctgag ctgtcctcat cccaggctga tacaaaataa ggttccagga
    12361 agccttattt tcttacatta agacgtaaat ccctaaaact tttgtctcaa ctttttcaaa
    12421 gtacggccga taagtctaat atctagtcct gagacaaaca ggatagcaac aagagggaga
    12481 aatccaaatg gccgaaaaca acagcagtca taaaattgaa aaacaaattc cacataatgt
    12541 ggttcaactg ggcccttcaa aatgctgcgg cgaaggctgc aataaaaaac cagagaaagc
    12601 gggattttgt ggtgagcatt ttgagtggtt caaagctgga ttgctgacta aagacggcca
    12661 taaagctgca gattttgaga aaaaacatta ccactttatg aaagccaaaa aatcggctgc
    12721 gtaagggggc ttaataaaca ccggaaactt tccaaaaacc ttgcgattcc catttccgtt
    12781 atgccaggcc acttgagcct ggcatttttt ctgtattaaa gagttcccca aaaaaacatg
    12841 aaaaatataa atccgaagat aaaataccta aaactgactt ttaatgcttc ttgtaacgtc
    12901 acggggacct ctcttcttct cttcggtcaa atcccctgct aacataaccc tctagacttt
    12961 tgatgagata ttggatggag ctttagagga gtttttatga agaaagccgt tttgataaca
    13021 ggtgcaagtt caggaattgg gcaagccacc gctttggagt tttctcgcca gggatatttt
    13081 atttatctta tgggtcgcaa caaagagcgc cttgaagaaa cggcgctgcg ttgttgtgct
    13141 ggtgcctgct tgctcagttg tgatttaaaa tatcccgccc aagttgaaaa gcgtctcaat
    13201 gaaatttatg aaaatccact ctatcgcttg gaaattttga tcaacaatgc cggggttttc
    13261 aagactcata gcatggaaga aggttccgat gagatttgga cagaacagtt tgaaaccaat
    13321 cttctcgggc ctgtccgcct gactcgtctt ttgatcccct actttaaaaa atatcaaaaa
    13381 ggttctattg taaatatctc gtccaccttg ggactgcgcc cctcttcaaa taccggggcc
    13441 tattccgctt tgaaggccgc cctgatcaat tggactcaaa gtctcgcgca agaagccggt
    13501 gcctacggag ttagagtcaa ttgcatctgc cctgggatcg ttgacactcc tattcattct
    13561 ttcaataatc tgcccgagtc gcaaaagcaa aaagttctgg aggagatggc gaaacttcaa
    13621 cctctgggac gcattggcac ccctgaggac ctcgccaaaa gtgtttactt cataggctct
    13681 gaactctccg cctggaccac gggcgcggtg ctctccgtag atggaggaat ttatctgcaa
    13741 tgacaaaaac tttcattgtg acttcatctt tttcagcagc tcacttttat cgccagaaaa
    13801 agtggtcctc gcttgaaaat caaatttgtt ttggcaaatg ctttactgaa tatggccatg
    13861 gccataatta tcgcctagag gtcggttttc agactccgca ggcgctactc ctggaccttc
    13921 agcaactggt gaaagccatc acggacccat tggatcacca gcatcttaat ttcgtgatcg
    13981 cggaatttaa agatttggtt ccgaccacag agaatattgc taaatatctt ttaaaaaaac
    14041 ttcatgaggg ctgtccaaat gagttcattt cttatataaa actttacgaa atggacgact
    14101 tatgggtgga gattcggata tgagcaatga tgaaaacaag ggcaagggcg aaggtaaaga
    14161 caaagttcgg ttatcgaaat taatggcgga aaaaggcctc tgctctcgtc gtgaagccga
    14221 tatctacatt gccgagggcc ttgtttacgt caacggagaa aaggtttctg tccttggcac
    14281 caaggtctct ccgcaagatc gagtgactct cgatgcaagg gttctgaaaa cccaaaacaa
    14341 tctggcgact attttactta acaaacccgt gggctatgtt tcggcacaac ctgagccgca
    14401 gtacactccc gctattaagc tcctcactcc tgaaaaccaa tttggtgtta gtaaacaaaa
    14461 attgaaatct gattcttttg ccggccttgc agtcgcaggt cgcctggata ttgattctca
    14521 aggtttattg attttcactc aagatggacg cattgctaaa caaatcattg gagagaacag
    14581 caaaattgat aaagaatatc tggtccgcgt cgagggaatt cttcccaaag aaaagcttca
    14641 gcttctccgc tatggactgg agctggatgg caaaaagtta aaacccgccg tcgttgaatg
    14701 ggtcaatgaa gatcagcttc gattcatcct caacgaagga aaaaaacgtc aaatccgccg
    14761 catgtgcgaa ttggtgggcc tacgcgttcg aggccttaaa cgcgttcgag tcggaaaaat
    14821 caccctcggg cagcttcctg aaggcaaatg gcgttttctt gaggccgacg agagccttga
    14881 ttgacaacct agtccacttc attaaacaat cctcctgtag aaaaaatgtc ttttggggga
    14941 ttcatgttaa taaatatcag gttcaacatt ggctcagtta tttcttcagt gcttggttcc
    15001 atttttttaa gcggtctgat ctctttaccc gtggctcagg caaaacccga aggcaaaatt
    15061 ctggtttatt gctcggaagg aaaccccagc tctttcaatc ctcaactcgt cagcgatggc
    15121 acttctttaa attccaccca tcctgtttat aacggtctag tggaatttga gttgggatcc
    15181 acgaaagtga ttccgatgtt agcggagtct ttcaccgtat ccaaagacaa actcacttat
    15241 acgtttaaac ttcgcaaagg ggttcgattt cacacgacca aaaatttcac tcccaaaagg
    15301 gactttcaag cggaagacgt tctcttctct tttgaaagac aatggaaaaa aaatcaccct
    15361 taccataatg taggcggggc tacttatgaa tacttcgaag gtatggacat gggtaaagtg
    15421 atcaaatcca taaaggctct caccccgatg accgttgaga tcaccctgaa ccatcctgag
    15481 gctcctttta tcgcaaatct ggcaatgccc tttatgaata ttctctcaaa ggaatatgcg
    15541 gatcagttga cagctcaaaa caaacaggcg gatattgatt tgcttcctgt gggcacgggg
    15601 cctttcgtat tccaatcctt tatcaaaggc agctcgatta aatacaccag ctttgaaaat
    15661 tattggggca agaaaggaaa tatcgagaaa ttgatttttg ccatcactcc cgacgccaac
    15721 gtgcgcacgc aaaaacttaa gactggagaa tgtcagttcg ccaccgagcc cgcccccact
    15781 gatttggcat cactgaaagc ggatgtaaaa atatctgtcc tcaatgctcc cggcttaaac
    15841 gtcggatact tggcaatgaa tgtaaccaag aaaccttttg ataaccttct ggttagaaaa
    15901 gcattgaact atgctttaaa ccgtaaagct tatattgaag ccatcttcct ggggaatgcc
    15961 atcgttgcaa aaaatcccct tccaccgacc atttggtctt acaatgagag tgtaaaggat
    16021 tacgattata atccggaaaa agccaaggaa ttgctgaaac aagccggttt tcccaatggc
    16081 tttgaaactg aaatgtggac catgccggtt tctcgtccct acaatcccaa cggcaaaaaa
    16141 atgggcgaaa tgatgcaagc ggatcttgtc aaagtcggaa tcaaagtaaa acttatcacc
    16201 ttcgactggc caacttatct ggcgaaatca aaaacgggtg aacatacttt aatccaattc
    16261 ggctggactg gagataatgg cgatcctgat aattttctca atgtgctttt gggatgctcc
    16321 agtgtggaag cgggttctaa cttttctcgt tggtgccatc aacccttcaa tgatttgatt
    16381 ctacaggcga aggccaatcc agatatcaaa aaacgcaccg agctttacaa aaaagcccaa
    16441 gtgatcttta aagaagaagc cccgtgggta accctcgatc actccacagt ttttcgggcc
    16501 atgtcgaaga acgtgaagaa ttataaaatg gatcctttgg gtgcggatat ctttaacaac
    16561 gtcaacattg agtagttagg ccctcggatg tttttatttt taagtaagaa aattctagaa
    16621 gtgagtttca ccttggtggg cattactttt atatgttttc tactccttcg cctagtccct
    16681 ggagatccgg ttctactcat gatcggtgag cgcaatatgg accccgtggc ctatgcggaa
    16741 gctaaacaaa aacttggact cgacaaacct gtcctggtgc aattcggaaa atatattgag
    16801 caggtagcct ccggggatct tggcacttct gtcacttcta aaacctcggt ttggactgag
    16861 tttaaggagc gtttccccgc caccgtggag cttagttttg tagcattgct ttttgcaact
    16921 ctctttgcga ttccctttgg agttttggcg gcgctcaaaa aaaacacctt ctttgattat
    16981 ttcctcatgg ggtcctctct gactggatac tccatgccga ttttctggtg gggcttggtt
    17041 cttattttac ttttctccgt caagcttggt tggaccccgg tctcaggacg tatcgctgtg
    17101 gaatatgata ttcccctgaa aacaggtttt tacctgctcg attctttaca gagggacgtt
    17161 cgcgaagccg agggcttaaa acctttctgg agtgccgtga gccatctcat ccttccgagc
    17221 attgttttag gcaccatacc ccttgcggtg atggctcgca tgactcgctc gagtttttta
    17281 gaagtcctgg gtgaagacta tattcgcaca gcacgcgcca aagggctttc cgagtatcgc
    17341 gtggtgctga ttcactcttt tagaaatgcc ttgattccta ttgtgaccac gctggctctt
    17401 tgtttcggca gcattatcac cggtgcgatt ttaacagaaa ccatttttag ttggcccggc
    17461 attggtcgat ggatcgtgac gagtatcacc tcccgcgatt accccaccat tcaaggtgga
    17521 gttttaatta tctccgttat aataatcatt acgaacacct tggtagactt actttatatt
    17581 tacctcaacc cgcgtattaa aaacgcttta ctaaagtagc ctatgaaaga ctttttttct
    17641 gagcttttaa aagatatcaa aaagaacaaa ggagccttcg cgggcctttt gctgatctta
    17701 atttttattc ttttagcgat ctttgcgcct ctgctctcgg tttatgctcc agaccaggtc
    17761 attgaaggag ccctcaggct tccaccgaat tctcactttc aaaattatta ttttattttt
    17821 ggcacggatg atatcggtcg ggaccttttc acgcgacttc tgtatggctc tagaatctct
    17881 cttttggtgg gcctcagcgt tgtggtccta gccgcgtcct tgggaacttc cctgggtttg
    17941 atttcaggtt attttggagg ctggacagat cggattattt ccagggccat ggattttatc
    18001 atggctttgc ccagtgttct cttcgccatc gtgatcgtcg ctgttctggg gccagggctt
    18061 atgaatgcca ttgtggcggt gtccattgtg gctctgccga actttgtgcg tttgattcgc
    18121 gctcaagtcc tcgctgaaaa aaacaaacag tatgttcagg ccgctcaact ttatggagca
    18181 agccatctgc gtattttatt taaagaaatt ctgcctaact gcatggctcc tttgatcgtc
    18241 caaggaagcc ttggatttag cgatggaatt ttaaactgtg cggccctggg atttcttgga
    18301 ctgggcgccc aagccccgac cccggaatgg ggaaccatgc tttcagatgc tcgaagtttc
    18361 ctggagtcct ctccgtggat ggtgaatttg ccagggcttt gcattcttac tgtcgtctta
    18421 tcttttaatt tattgggtga tggcctgaga gatgctctag accctcgcct taaaaaccgt
    18481 tcttaaatga ggttgccatt gcacctaaac aataagctcc tagaaatcga aaagctttca
    18541 gtgcaattta aaacagactc gggagcgatt gaagctcttc gtccgatttc tttcacagtg
    18601 ctggaaggcg aaattctcgg agtggtgggt gagtcaggtt ctggcaagag cgttacaaat
    18661 ttagcgctca tgggccttct tccggcaaat gctcaaatca ccgcacaaaa agtagttttc
    18721 aataaaaacc ctctcaattt aaattcacaa aaagacttac gcgctattcg tggctcccag
    18781 attgcaatga tctttcaaga ccccatgaca gctttgaatc ccagcctgac tgtgggctat
    18841 caaatgatcg aaactctgcg ctgtcacttt gatatttcaa aaaaagtggc ggaacaccgc
    18901 tccctggctc tacttaaaag agtggggatt tcaaatcctg aagctcgctt aaaatcttat
    18961 ccttttgaac tctctggggg catgagccaa agagtgatga tcgccatggc gatcagttgc
    19021 caccccaagc tcttgattgc tgatgagccc accaccgctt tggatgtaac tatccaaaaa
    19081 cagattttgg atctgctttt ggatttgcaa aaagaaaata agatgtcgat gattttcgtc
    19141 agccatgact tggcccttat taaaaaatac gcccatcggg ttcaggtcat gtatgcgggt
    19201 gaaatcgttg aaggtggcaa tacggaggaa gtgattaaga ctccccgtca cccttatact
    19261 ttcggattgc taaaggctcg tccgagtgcc catgagggga aaatgaaaga acctctctat
    19321 gctattcccg gagtggtgcc ctccctcaga gagcgcccca gcggatgcca atttcatccg
    19381 cgctgtttta gagccactga gctttgtaaa aaacaagccc cgctactcac gaccgctgcg
    19441 aggaactttg catgccatca tcctttggag atcctatgag cctgctaaaa gtcaaaaatt
    19501 taaaaaagtc ttatgttaca aagaatcttt ttttcggggc aaaaaaagaa gagccggtct
    19561 taaagggaat ttcttttgag ctcgaggaaa atcaaatcct cggagtcgtc ggagaatcgg
    19621 ggtgcggaaa aagcactctc gccaaagtcc tcacgcgact ggaagaggct agtggcggtg
    19681 aaatcatctt taatggaaaa gatctaagat ctttctcctc taaagaattt ccgcatcaga
    19741 ttcaaatggt tttccaagat ccctttcaat cactgaatcc ccgcaaaaaa gcctgggaga
    19801 ttattgccga tccgattctg gtgacggaaa atatttccac cagcgaagcc aaacaaagag
    19861 ccctggcgat gatgcaaaaa gtaggtctgc tgacggagac cagcgagcgc tacccacatc
    19921 aattcagcgg gggccaaaga caacgcctgg gtattgcccg ggcccttatg ttgcgtccca
    19981 agctgctcat tttagatgag ccggtttcag cgcttgatgt ttcaatccaa gcccaggttc
    20041 tgaatctact cctggacctg caaaaagaat ttcatctgag tatgattttt atttcccacg
    20101 atctttccgt agtcaaacat atctctgata aaactctggt tctttatttc ggagaaatcg
    20161 tggaaatggg cccaagcact caaatctttt cagagcctca acatcagtat acgcagaaac
    20221 ttctggagag tgccccttaa gagatctcct taagggggga cacttctctt agttcgaagc
    20281 ccctaactgc tcgccttcca ctctgtagat tttttcagct tctgctcctt gagcttgagt
    20341 caagcaaaca tatcctcctt cgatagaagt tccaactggt aacatttcaa cggcgttggt
    20401 gcctgtttca gaataaacac gataagcaac tttattcaat tccgcacaat aagcggcagc
    20461 cttagcatcc atcgcattca aagttttgat ctctcgcttg gcctcagacc aaaccgggac
    20521 tcctacaaac ttaaagcaaa actgcggcgg taaatttcgt gaagtacctg ggggatagga
    20581 tttgcaattt tcttggtgca tggtcgtaag acctgaaacc gcaaaggaag tggttgaaaa
    20641 agaaatcagg ctgcaaaaac taaaaaatcc taagaaagtg cccttaaaaa ctgtcataat
    20701 aactctcctc aatattgatg attctgtttt aaaaccacgg gggagcccct ccccctaagt
    20761 ctaaatgcca ttcgttattt gctgtaagtg caattttctt ctgcactcca attgccttga
    20821 ccgtcaaaac gaactttatg acttctttta aggctgagca taagtgtatt gctcttcact
    20881 tctatcataa gctcatttag agattcatca agagtgacgc caaaagcttt cgtttttgaa
    20941 tttgatgtta actttaaaac atccccttct tgagcggcca aattggtggt tgagtacttt
    21001 tcgccatggg aactattctt agattcagag acctgtttac ccctattgat accagatata
    21061 atcacagcac cttcggcacc atcaagatta tagtaagatc ccaaatctct ggccccttct
    21121 accttgagag tatctgtcgc tagctttgaa atgaccaacg tagacgagca gaggctgctg
    21181 ccttcagaaa gatctgaact cttcaatgaa tattttccct caattttcgc ctgtgcaatg
    21241 gaagcacaaa ccatagataa aacgatagca gtaattttct tcatactgag actccttgat
    21301 accgttccaa atcctgtggg atttacttcc caattggcag gggcccggtt cctttgactg
    21361 atgccagatg cgatatccgt accaatatcc agccagagat aaaataactt tgaagcaaaa
    21421 gtggtgtcca aaaactttac aaagtgccgc aagttccgtg gaactttttc atttttcatt
    21481 tcatttttct atccgaatgt tttcattgga cttctggttg tactcaaaca gaagtaaagt
    21541 cgcgctacga gtttcagaat gaatgataag cgccttcgcg acttatgata gccgtatctt
    21601 cgtctagtag aaaatttaag acttctataa aacggaaagc taactttttc attttcattt
    21661 tgtcacttca ttttcacttc tcttccgaag atttgctctc tgaggaatat ttcccacggc
    21721 aattccatga agccctgcgt atatatttat tttttataaa atatctcttg cgacggtttt
    21781 tcgtctctct cagaaggcga tggcttggtt tttttattct ccacccgagg cctctcagat
    21841 cgcaaaacaa ctcttcacct cgacagtatg ggccgctctc aaacccttcc gcagagctat
    21901 tcatgaagtt ttcacctctg aaaaaaatca taaaaattcc ctctcaaaac tcgtggtatt
    21961 ctcttttttg caaaagcttt ttgaatttat tttttagact atttttttga taaggagcac
    22021 tctctgccaa caacttcccc tgaatacgcc ttggatctct caaaggtttc aaatcaagaa
    22081 atcctttttc gtcttgaaag gctttcgcgc acg
//
LOCUS       DATBTE010000358         1683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2029657, whole genome shotgun sequence.
ACCESSION   DATBTE010000358 DATBTE010000000
VERSION     DATBTE010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2029657"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VIG33_13480"
     CDS             complement(<1..238)
                     /locus_tag="VIG33_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271896.1"
                     /translation="MLQVLRKIPHSAGPQRLLFFHHAGGSGGQYLPALKPWASRYEIH
                     YLDLPGRFFRLNETPFADIQELIKAIKLQIEVLLP"
     gene            298..525
                     /locus_tag="VIG33_13485"
     CDS             298..525
                     /locus_tag="VIG33_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271897.1"
                     /translation="MEAASQNFWRKCSTCKKPIGLGAPYYVCSVSTCNGQRTGYVFCG
                     IQCFESHVPGARHKNAGAIEKTAPKIPYVGS"
     gene            complement(510..908)
                     /locus_tag="VIG33_13490"
     CDS             complement(510..908)
                     /locus_tag="VIG33_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEY8271898.1"
                     /translation="MDQNQSTAYELLGGEAVLKKITHRFYEVMDTNENFKGLRALHAA
                     DLQEANEKLFMFLSGWLGGPNLFVEKYGHPRLRARHLPFQIGKSERDQWVLCMVQAFD
                     DLQVAEPLRTNLLHSLLKLADHMRNIQEPT"
     gene            complement(921..1319)
                     /locus_tag="VIG33_13495"
     CDS             complement(921..1319)
                     /locus_tag="VIG33_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271899.1"
                     /translation="MKKEAILKVIAKDLGTDEVTQLNKLFKDISVPQIKFRMAGKNLG
                     HFGYTVNIKNISTEGLKVFLANKVKLIKFEDIEQFEKAKPRVERPLRKKEPVKLAEKK
                     RIAVKKEPPEKKMVRNSSLQGSRFIPPKSR"
BASE COUNT          476 a          340 c          389 g          478 t
ORIGIN      
        1 agggaagaag gacctcgatc tggagcttta tagccttgat aagctcttga atatcggcaa
       61 agggggtttc gtttaagcgg aagaagcgtc cagggagatc caagtaatga atttcatatc
      121 gcgaagccca aggcttcaga gccggaagat attgcccccc gctaccgccc gcgtgatgaa
      181 aaaagagcaa tctttgagga cccgctgaat gagggatttt tctaagaact tgaagcataa
      241 ttctagacac tctgacacaa ttgtttagag taataaagaa attgttaagg gggagtcatg
      301 gaagcggcat cacaaaattt ctggcgaaaa tgcagcactt gcaaaaaacc catcggtctc
      361 ggcgcacctt actatgtttg cagtgtttct acctgcaatg gtcagcgcac gggctatgtc
      421 ttctgcggga tccaatgttt tgaaagtcat gttccaggag cccggcacaa aaacgccgga
      481 gccatcgaaa aaacagctcc gaaaattcct tacgttggtt cttgaatatt tctcatatgg
      541 tcggcgagct ttaaaagaga atggaggaga ttggttctta gaggctccgc cacttgcaga
      601 tcatcaaagg cctggaccat acagagaacc cattggtctc tttcggattt gccaatttga
      661 aaaggcagat ggcgcgcacg taaacgggga tggccatatt tttccacaaa caaattgggt
      721 ccccccagcc atccagacag aaacataaaa agtttctcat tggcctcttg cagatccgcc
      781 gcgtgaagag cgcgaagacc tttaaaattc tcgttggtat ccatcacctc ataaaaacga
      841 tgagtgattt ttttcagaac agcttctccg ccgagaagct cataggctgt ggactggttt
      901 tgatccatat gaataatcta ttatcttgat tttggaggga taaaacgact tccttgaaga
      961 gaagaatttc tgaccatttt tttttctggc ggctcttttt tcaccgcaat ccgttttttt
     1021 tcagcaagct taacaggttc ttttttgcga agaggtcttt caacccgtgg ctttgctttt
     1081 tcaaattgtt caatatcttc aaatttaata agcttcactt tattggccaa gaaaactttc
     1141 agcccctcgg tggagatatt tttaatattc acggtatagc cgaagtgccc aaggtttttc
     1201 cctgccattc gaaatttgat ttgaggaaca gagatatctt taaagagttt attgagctga
     1261 gtgacttcgt cggtgccaag gtctttggcg atcactttaa gaatggcttc ttttttcatg
     1321 gatggccctt ccggcaaaaa gttggctttt tatacaaggg caggggcgga ggggcaagtt
     1381 aaatcagaag tccgagggaa agccaaagtt ctctatttgg tacgagaagg gtatttgaga
     1441 aagaggtgag aacgaggcat gtaaattata aatgcgacag atggaaaaag aataaacagc
     1501 ctatggatat tactccactt gaagtcattt atagaagctt tgacaaacgg tgtgaaagtt
     1561 gcttcttaat tcgttgaaat ggcaagacca gcgaacttta atttcagctc ccgatttcga
     1621 acagggatga ggtcttctca ttttcacgga actttttgga aaatagtcat tctgtttctt
     1681 acg
//
LOCUS       DATBTE010000359         1951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_935118, whole genome shotgun sequence.
ACCESSION   DATBTE010000359 DATBTE010000000
VERSION     DATBTE010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935118"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..>1951)
                     /locus_tag="VIG33_13500"
     CDS             complement(51..>1951)
                     /locus_tag="VIG33_13500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8271900.1"
                     /translation="LLNNDTIVTHHWLEDLIFYAKSTFVGPVCNSVGNEQIIEINYAT
                     HGFNGYTTQAVNSFAEHWRAHHLGNVEKTTMLGFFCVLGERSLFETVGPLDESFGLGY
                     YEDDDYCLRVNQMSFAQVIVRQAFVHHWGSASFKDLSAQALQKLFSQNRQKFIDKHGI
                     LPADHSHAWLKAILHEYDWLKSQNKKIDPIYAWVPSFWSSSFKHELLKKIEARISLHV
                     NPPFIRNFLIKCLAILRQHDVRTGAVRLFHRTLQQLSGKFIRFPMGRLILRSWSQKTI
                     VIFPIQRFNERMQRPQQLALQLAEAGYKVIWVNPDLGQASSRHHNIQRINKNLLILEL
                     KGICYANFYTSSLSKKDANLIVNAINKLLPNVELSDRTALFQSPFWHTMDSHQFNQSV
                     YDCMDAHEAFGSATLEVSAIEDQMFKKATKITTSSSYLKDKIKKEFGRDAVIIRNACN
                     PKDFEPTPSPHSKKNPTVGYFGAVAEWFDFDLLIQVAHRLPNHDFVIIGNTAHSNLEK
                     KTLPSNIFMKGEVPYNKLPEIASSWDVAIIPFLLTELIQATNPVKLYEYYALGLPVVA
                     TSIPEVMECPVKAYTATTDREFAEKIKSAIKENLAEKRSERQIFAQSQTWKHRATDFI
                     QCLESAQC"
BASE COUNT          559 a          379 c          430 g          583 t
ORIGIN      
        1 ttgcatatat ccatcctgct ggtcaaagct cggatccggt gacataatga ttagcattgt
       61 gcagactcga ggcattggat aaagtcagtt gcgcgatgct tccaggtttg cgactgagcg
      121 aagatttggc gttcggaccg cttttcggct aaattttcct taatagctga tttaattttt
      181 tctgcaaact ctctatctgt agtcgcagta taggctttaa cagggcactc catgacttca
      241 ggaatggaag tagcgacaac tgggaggcca agggcgtaat attcatataa cttcactggg
      301 ttcgtggcct gaatcaactc agtcaataaa aaaggaataa tagcgacatc ccaagaggag
      361 gctatttcag gtaacttatt ataagggacc tcgcccttca tgaaaatatt cgaaggtaaa
      421 gtttttttct ctaaattaga atgagcggta ttcccgatga ttacaaagtc gtggttaggc
      481 aatctgtggg ccacttgaat caataaatcg aaatcaaacc attcggcgac ggctccaaaa
      541 tacccaacag tgggattttt ctttgagtgc ggtgaagggg tcggttcaaa atctttagga
      601 ttgcaagcat tccgtatgat gacagcgtct ctgccgaatt ctttttttat cttatctttc
      661 agatacgatg aagaagtggt tattttggtg gcttttttaa acatttggtc ttctatggcg
      721 gaaacttcca aagttgcgga gccgaaggcc tcatgggcat ccatacagtc atagacagat
      781 tggttaaatt gatgagagtc catcgtgtgc caaaacggac tttgaaataa tgccgtacga
      841 tcgcttaatt ctacattcgg tagaagttta ttgattgcgt tgactattaa gtttgcatcc
      901 ttttttgaga gcgaggaggt ataaaaattt gcataacaaa taccttttaa ttccaggatt
      961 aaaagatttt tatttattct ttgaatgttg tggtgtcggg agctggcttg accgagatcc
     1021 ggatttaccc aaataacttt ataaccggcc tctgccaatt ggagcgccag ttgttgggga
     1081 cgttgcattc tttcattaaa tctttgaata gggaaaatga caatggtctt ttgactccac
     1141 gagcgtaaaa taagtctccc cattggaaaa cgaataaact tgccggataa ttgttgaaga
     1201 gttctatgga agagcctgac agcgcctgtc ctaacgtcgt gttggcgtaa aatagctaag
     1261 cactttatga gaaaattacg aatgaacggg gggttcacat gcaacgaaat tcgcgcttca
     1321 atttttttca gtaattcatg cttaaaagaa gaggaccaaa atgaggggac ccacgcataa
     1381 atagggtcta tttttttatt ttgtgatttt aaccagtcgt attcgtgcaa tatcgcttta
     1441 agccaggcat ggctatgatc tgctggcagt atcccatgtt tatcaatgaa tttttgacga
     1501 ttctgagaaa ataatttttg caaagcttgt gctgaaagat ccttaaaaga agcactcccc
     1561 caatgatgta caaatgcctg tctcacaatg acctgagcaa agctcatttg attcacgcgg
     1621 aggcaataat cgtcatcctc atagtagccc aggccaaagg attcgtctaa aggacctacg
     1681 gtttcaaaga gacttcgttc gcctaagacg cagaaaaagc cgagcatagt ggttttttcc
     1741 acgtttccta aatggtgggc tcgccagtgt tcagcaaagc tatttaccgc ctgggttgta
     1801 tatccattaa acccgtgagt ggcataattt atttcgatga tttgctcatt ccccacacta
     1861 ttacagacag gaccgacaaa agtgctttta gcataaaaaa taagatcctc tagccaatga
     1921 tgagtgacaa tcgtatcatt gtttagaagg c
//
LOCUS       DATBTE010000360         1548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_322153, whole genome shotgun sequence.
ACCESSION   DATBTE010000360 DATBTE010000000
VERSION     DATBTE010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322153"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VIG33_13505"
     CDS             complement(<1..680)
                     /locus_tag="VIG33_13505"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEY8271901.1"
                     /translation="MSYTIEVYFGNQKAEKDFSVFALYVLYFPQLVAGPIERPQNLLH
                     QFRRMGVPDNQTIRSGLFLIFTGLAKKCIVADNLTILSDKVFSNPQAFGTLAHFLGTL
                     FFAFQIYCDFAGYSDIARGCSKFFGIELMLNFNKPYFAKTVSEFWRRWHLSLSTWFRD
                     YVYFPLGGNRNSQYRTIFNSLVVFTLSGLWHGANWTFIFWGLFHGLVVGLEHLLKKSD
                     SRLWTIMLV"
     gene            complement(1291..>1548)
                     /locus_tag="VIG33_13510"
     CDS             complement(1291..>1548)
                     /locus_tag="VIG33_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271902.1"
                     /translation="GSVQGQATKKNGLTASSTDAGHVHSLAYPSGGGGTMAIQPLAGN
                     GSYIGSMAGMATGYANITTTIGAGDSETRPVNANVNYIIKL"
BASE COUNT          537 a          319 c          270 g          422 t
ORIGIN      
        1 accagcataa ttgtccataa ccgagaatct gattttttta acaaatgctc cagcccgacg
       61 acaagaccat gaaaaagacc ccaaaatatg aaagtccaat tcgctccatg ccacaatcca
      121 ctgagtgtaa aaaccactaa ggaattaaag atcgttcgat attgagaatt cctatttccg
      181 cccaatggaa aataaacata atcacgaaac cacgtcgaca atgataaatg ccatctccgc
      241 cagaactccg aaacagtttt ggcaaagtaa ggtttattaa aatttagcat aagctcaata
      301 ccaaaaaatt ttgaacatcc ccgtgctata tcggaatacc ctgcgaaatc gcagtatatt
      361 tgaaaggcaa agaatagtgt tcccaggaaa tgcgccaatg tcccaaacgc ctgcggattt
      421 gaaaaaacct tatcacttaa aattgtcaaa ttatcggcaa cgatacattt ttttgccaac
      481 cccgtaaaaa tcaagaacag accactccgt atcgtttgat tgtctgggac acccatacgt
      541 ctgaattggt gaagtaaatt ttgaggtcgc tcgatggggc ctgccaccaa ttgagggaaa
      601 tagagaacat aaagagcaaa gacagaaaag tctttttccg ccttttgatt tccgaaatag
      661 acttctattg tataactcat cgattgaaaa acatgaaacg atagtccaat aggtaacaga
      721 aatgacaggc tactaagtct gtagttccaa tcaaaaattt ttgccagtgc agataaattt
      781 tcattgaaga aattaaaata cttaaaaaag aatagaattc ccaagttaga cattagacta
      841 ataattagaa atatcttttt gtttctcttt tctgcgcgtt caattgcgat cccggaaaaa
      901 taatcaatga taatcacagc aaaaagtatc agcagatatt taggaataaa ctggctataa
      961 aaataacagc ttgcaagaat taaaaagtaa acttgcgact tccctcttaa aagcgcgaaa
     1021 acaaagacta caatggtaaa aaaaacagca aaggatagag agttaaaaag catgtctttc
     1081 ccaatatctc aagacggtta aagatatcaa gattaacatg tgtttatttg catgtaaatt
     1141 agtgtcgatt caatgagatc taatgtcaca tctcgggctc agtcacttta aatttaacgc
     1201 cccgcccctc actaatggct tcatttaatg atttagatgg gttgacgtaa aaagaggcat
     1261 cctttcattg aaagatgcct ctctaattta tcaaagttta ataatgtagt ttacgtttgc
     1321 attgaccggt cgtgtttctg aatcacctgc cccgatagtt gtcgtaatat tagcatatcc
     1381 ggtggccatt ccagccatac ttccgatata tgaaccatta ccagctaacg gttgaatcgc
     1441 catagttcca ccgccaccag atggatatgc caaggagtga acatgtcctg cgtcagttga
     1501 ggatgccgtt aatccattct ttttcgttgc ctggccttga acagaacc
//
LOCUS       DATBTE010000361         1636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_117073, whole genome shotgun sequence.
ACCESSION   DATBTE010000361 DATBTE010000000
VERSION     DATBTE010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117073"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VIG33_13515"
     CDS             <1..720
                     /locus_tag="VIG33_13515"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HEY8271903.1"
                     /translation="NRREFKLLPFALREQKFKDAEETILRELVASHPAEFASDASTLE
                     QLARVQHYSLPTRLLDITWNPLVALYFAAKDDQGTTGEVIVCRIKKHHVKFFDSDAVS
                     CIANLAHLKISEKVAIDFTLTGDNFNSQNPIDRLLQFIRVEKPHFRAGINPAHLKTVL
                     CVKPKKNNPRLLAQSGAFLIFGLTDSLDTMPVTEISVERIQISGRKKSDIVKELDRMG
                     INDSTMFPEIEKAALYIRRAL"
     gene            950..1171
                     /locus_tag="VIG33_13520"
     CDS             950..1171
                     /locus_tag="VIG33_13520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEY8271904.1"
                     /translation="MSKITATDTLELSIPERIQLVEDIWDTIAVKASSLELTDEEKSI
                     IDARLEKYHQNPESGSPWEDVYKRIVSKQ"
     gene            1168..1473
                     /locus_tag="VIG33_13525"
     CDS             1168..1473
                     /locus_tag="VIG33_13525"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY8271905.1"
                     /translation="MKYSVIVSPEAENDLKEIFSWYEDTRIGLGYDFLLQVDAGINFI
                     KRNPEIHPIEYKGTRKHIIKRFPYKIVYFVTEEKIIILAALHGKRSFELLKNRIDSV"
BASE COUNT          518 a          365 c          354 g          399 t
ORIGIN      
        1 aatcgtcggg aattcaaact tcttcccttc gctctacggg aacagaaatt caaggatgca
       61 gaagagacca tcttacgaga gcttgtcgcc tcacacccag cagagtttgc gagcgacgcg
      121 tcaacgcttg agcaactcgc aagggttcaa cactattcgc tgcccactcg actgctcgat
      181 attacatgga atccccttgt agcactttac tttgcagcca aagacgatca aggaacaaca
      241 ggagaagtca tcgtctgtcg tatcaaaaag catcatgtca agttcttcga tagcgacgca
      301 gtaagttgca tagccaatct tgcgcattta aaaatctctg aaaaggttgc cattgacttc
      361 actctcacag gagataactt caattcacaa aacccaatag atcgcctact tcaatttatt
      421 cgagtggaaa aaccgcactt ccgcgcgggg atcaacccgg cacacctaaa gacggtgctg
      481 tgcgttaaac cgaagaagaa taatccacgc ctgttagcac aatctggcgc attcctaatt
      541 ttcggcctaa ctgactcgct tgataccatg ccagtcactg aaatttctgt cgagcggatt
      601 caaatcagcg gacgaaaaaa gtctgacatc gtcaaggagc ttgatcgcat gggaatcaat
      661 gacagcacaa tgttcccgga gattgaaaag gcagctcttt atatccggcg agcactatga
      721 aaagctgtaa ggcggaaact tgtagggtgg attcgccgct aacccggcag gggcttcatc
      781 gcgccccttt tcggctggaa atcttgatac tgaaatgggt tacccgttgg aatattttaa
      841 aatagttcgg tcattctggt attcgtaaat ggtgcgcggt gcgcccccta ctaggcttac
      901 aagtgtgacg attcagaaac tttttagatg gtaaaaagag gaacagaata tgagcaaaat
      961 aactgcaaca gatactcttg aattatcaat acctgaaagg attcaattgg tagaagacat
     1021 ctgggatacg atagctgtaa aagcatcttc tctcgaactg accgacgagg aaaaaagcat
     1081 aattgatgca cggctggaaa agtatcacca gaatcctgaa tcaggctccc cgtgggaaga
     1141 cgtctacaaa agaatcgtga gtaagcagtg aaatattccg tcattgtaag ccccgaggct
     1201 gaaaatgatt taaaggagat attttcttgg tatgaggaca ccaggatagg cttgggatat
     1261 gactttcttt tgcaggttga cgctggaatc aactttatca aaagaaatcc tgaaattcat
     1321 cccattgaat acaaaggaac aagaaagcac attatcaaaa gatttccata caaaatcgtc
     1381 tattttgtta cggaagaaaa gataataatc cttgcggcgc ttcacggtaa gagaagcttt
     1441 gagctactaa aaaataggat agacagcgtt taacaagttg caattcccac ttaatccgcc
     1501 tccgtttaaa cttgtcgcat cactttggca tcgtaactac aagacaacat ccttaaagga
     1561 gaaaaacatg caatccgcta aacaagccgc aaaacagatt attgatcatg tttctgacca
     1621 ggcaacgtgg gatgac
//
LOCUS       DATBTE010000362         7153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_935318, whole genome shotgun sequence.
ACCESSION   DATBTE010000362 DATBTE010000000
VERSION     DATBTE010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935318"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="VIG33_13530"
     CDS             <1..1007
                     /locus_tag="VIG33_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271906.1"
                     /translation="FAFEHKLPFAIKDSTGGPNQITHQMSRFNDITDPLQRVRKFIEF
                     VGSTTSTKSLPNDTYPVAVNRTYIHPGTVWSRPRITKDNFWRRLVGGSVQEDPGHAEL
                     VKEVADTGAVYLIGSTVPKEIRKLNTTSSLVFMPVETTTGFRNWIQPPYYTRSIETLP
                     GYSLQQFNDIGRGWAGTRSLSNWTSQVQSRLALREETRIEKITRQVENICRLVNSRVD
                     IVLKAEAHRIKVGGACMNINDYDSYSTPSRDKRIITTLKQLTSVAGRMGITAAQRAKP
                     LKPFLKDCPNIQIAPTQFITLYDFSIRALKGKVSSNPNDSFGARWGLEESTSNCPEYE
                     "
     gene            1000..1719
                     /locus_tag="VIG33_13535"
     CDS             1000..1719
                     /locus_tag="VIG33_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271907.1"
                     /translation="MNKNYRFILVLLMGGAALWTILHFSSPSEIDNIPAPGTPTEMPL
                     PAGDGHQAVSSPTPRPTPTPEVEVTSFGSHLRKIGDCLQIRNSLNKDAELKFSALDDS
                     LRGELGELISHNLDWKNVHILLPTGEKRRLRMEVEATGEEATGIRLHYYGVDNEDLPV
                     PLPLTDEQSLNPSETFIASLENEGKVTLREGAHRGIYNRGAELYYSERNGILSDLEIS
                     YQGKSVKCQNLQSAKGICSCF"
     gene            1798..1971
                     /locus_tag="VIG33_13540"
     CDS             1798..1971
                     /locus_tag="VIG33_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEY8271908.1"
                     /translation="MLRAAIAFFILAIVAFIFGAAGIAGVSMDIGKLLLTIFLILAVI
                     SFIIDLVGRRGHR"
     gene            2077..2814
                     /locus_tag="VIG33_13545"
     CDS             2077..2814
                     /locus_tag="VIG33_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271909.1"
                     /translation="MRSFFTLFILSISFFAQAQTVEKITGNEALVTVLASESLTVGDK
                     VNFLNENLNISGQGEVTKLSSGGKKAIVKIISGNAKPGMSLETLSATPRPNKSLMVDT
                     LDEEDRRILHIGEISTSSYVIGGILGTYPLGLGIGHAIQGRYMDKGWIFTVGELGSLA
                     VVMGGVGSCLDSISNSSRCNGGAWFLGAMGYVGFRIWEIIDVWAAPPEINRRYHNLKF
                     RMESRAILSPLLAPTRDGAVFGLRMTF"
     gene            complement(2824..3192)
                     /gene="rnpB"
                     /locus_tag="VIG33_13550"
     ncRNA           complement(2824..3192)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VIG33_13550"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            3304..3747
                     /locus_tag="VIG33_13555"
     CDS             3304..3747
                     /locus_tag="VIG33_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEY8271910.1"
                     /translation="MKIFVSVLVFVTALTVGVQSLAMNKEGSIDMDGLFPPPQSNLSF
                     GERPAKPELTAPSFFQKINASQATLTWKAVPTAESYHVQLATDPNFKWLVKEEQLLKG
                     TTLEVAGLESGKHYFWRVSAMKPENKNGHIQGAFSSSMFVTSATK"
     gene            3785..6091
                     /locus_tag="VIG33_13560"
     CDS             3785..6091
                     /locus_tag="VIG33_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid cis/trans isomerase"
                     /protein_id="HEY8271911.1"
                     /translation="MNWLISIFIIFNVHTLAQAADAFVDLYTNSVQPLFNSRCIACHS
                     CLNSPCQLNLQSYEGFQRGANKVNIYNGNHIDAVSPTRLWIDAHTPQEWQSRGFFSVN
                     TSKEPANNLFFQAVAIKASEKSAPTKTVFESQSCPATPTEIKVAMPYALPGLKVEELK
                     ILKKWIQEGAPGPNAEAQKTLSFVAPEIKKQIQSWEKFLNENDLRHKLVSRYLYEHLF
                     LAHIYFPQEPQTFFRLVRSSSKCGKTIDEIATRRPNDNPGTESFQYCFKKFVGTVVSK
                     THMPYELSPAKKERFENLFFKTHWKVTRLPTYNYQEAENPFLAFADIPPKSRYQFLLD
                     DAQYEVSTFIKGPVCNGSVAVNSIQEQFFMFFINPESEIMTRARGFQDQAQPLLILPG
                     VWGSDVKLGDTPSFLTKLTEHREKFRSLRARWKKELFPKGYALADIWDGDGENPNAVL
                     TILRHDDNAAVMTGAIGDLSKTIVMLDYALFERLVYNLVVNFDVFGNISHQLLTRDYM
                     DMLRMEAEEIFLSFLPPEERLPTRDSWYKGFAAELKMKYMYPVLEKDLPTSVVYTSPK
                     NSKKEFVEKVLFARMNEKVRGAMDFINWKNIDIPENVLTQTEKAQRETIPIDSELRKI
                     AAVAAQDKTPFAQFFPDVALLQVKGKMGEQKVYSLIHNKEHQNISWILAESMRMSPEE
                     DSLVIREGYWGSYPNMFFTVSEEQMSAFTSQVLKLKSAKDYAKLVRNFGVPRTQERFW
                     SVYDEINAHFRKTAPTQFGYLDITRYEM"
     gene            complement(6098..6724)
                     /locus_tag="VIG33_13565"
     CDS             complement(6098..6724)
                     /locus_tag="VIG33_13565"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY8271912.1"
                     /translation="MNYTMSGYGAVDYAGFWRRAGAVIIDGIIVGLISLLFKMVALNS
                     MGIDILLAILYTPFFESSELQATPGKALLDMRIVSTSGERITFKKAIIRYFVRIVSGL
                     ILCFGFLMMLFTEKKQTLHDLAAETLVVRGEVKNVNYFQAWYHQVLQVLGMLDKVPAK
                     SNESLGPQEGAGQDVTPADLANLYELYQKGILSEGEYNQKREEILKKL"
     gene            complement(6765..>7153)
                     /locus_tag="VIG33_13570"
     CDS             complement(6765..>7153)
                     /locus_tag="VIG33_13570"
                     /inference="COORDINATES: protein motif:HMM:NF025795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Bd3614 family nucleic acid deaminase"
                     /protein_id="HEY8271913.1"
                     /translation="ASKLAWIRKIARLNIRGQVLHDYDRDIACLLTDHSGNMLAYGVN
                     SNSKNKTLHAEVNTVHRFYHDHRKKLPVGTKIYTTRKPCKMCAGMIYDWSEDPASLEI
                     HYLEEDKSSQNSVLEQVVKWIRLDSE"
BASE COUNT         2005 a         1693 c         1512 g         1943 t
ORIGIN      
        1 tatttgcctt cgaacacaag ctgccctttg ccattaaaga ttcaacagga ggcccgaatc
       61 agatcacgca tcaaatgtct cgcttcaatg atattactga cccccttcag cgcgtgcgta
      121 aattcataga gttcgtagga tctacgacca gcacaaaatc ccttcctaac gatacatacc
      181 ctgtcgccgt gaatcgtact tacatccatc ctgggactgt ttggagtcgt ccacgaatta
      241 ccaaagacaa tttctggcgc cgtcttgtgg gaggctcggt tcaagaagat cctggccatg
      301 cagagctggt aaaggaggtg gctgataccg gagctgttta tttaatcggt tccacagtgc
      361 caaaagagat tcgtaaactc aacaccacct caagccttgt gttcatgcct gtagagacca
      421 ctacgggttt tcgtaattgg atacaacccc cttactacac tcgctctata gagacccttc
      481 caggctactc cttgcaacag ttcaacgata ttggacgcgg ctgggctggc actcgctctt
      541 taagcaattg gacctcccag gttcaatctc gtttagcgtt acgtgaagaa acaagaatcg
      601 agaaaatcac tcgccaagtt gaaaacattt gtcgtctggt aaacagtcga gtggatatcg
      661 ttctcaaggc tgaagctcat cgtataaaag tcggtggcgc ttgtatgaat ataaatgact
      721 acgattctta ctcaacccca tcccgtgaca aacggatcat tacaactctg aaacagctta
      781 ccagcgtggc tgggaggatg gggataactg ctgctcaaag agcaaaaccg ttaaagcctt
      841 ttctaaaaga ttgtccgaac attcaaatcg caccaaccca gttcatcact ttgtatgact
      901 tctcgatccg ggccttaaaa ggcaaagttt cttcaaatcc taacgacagc ttcggcgcac
      961 ggtgggggtt agaagagtcc acttcaaatt gcccagaata tgaataaaaa ctatagattt
     1021 attttagttc ttctcatggg tggagctgct ttatggacga ttctccattt ttcgtcccct
     1081 agcgagattg acaatattcc agcgcccggc actcccacag agatgcctct tccagccggg
     1141 gatggacatc aagctgtttc atctccgaca ccacggccaa ctccaactcc agaagttgaa
     1201 gtcacttctt ttggatctca tttacgcaag attggcgact gtctgcaaat tagaaatagt
     1261 ttaaataaag atgccgaatt aaaattttcc gcactggatg attcactccg aggagaactc
     1321 ggagagttaa tttctcataa tctagattgg aaaaatgtgc atatactttt gcccactggc
     1381 gaaaagcgcc gccttagaat ggaggtggaa gcaactggag aagaagccac aggaattcgt
     1441 ttacactatt atggcgtgga taacgaagat cttccggtcc ctctcccatt aacagatgag
     1501 caaagcttga atccctcaga aaccttcatt gccagccttg aaaacgaggg taaagtcacg
     1561 cttcgcgaag gggcccaccg tggaatttat aatcgagggg ccgaactcta ttatagcgag
     1621 cgaaatggaa ttctttctga ccttgaaatt agctatcagg gaaaaagtgt aaaatgccag
     1681 aatcttcaaa gcgccaaagg aatttgtagt tgcttttaga agttttgctc acagtgccca
     1741 cttacataga accccttagc tggtctgagc tatactttgg tcatataagg aggttctatg
     1801 ttacgtgcag ccattgcctt ttttattttg gccatcgtcg ccttcatttt tggagcagct
     1861 ggaatagcag gagtttccat ggacatcgga aagctcttac taactatttt cttaatcctt
     1921 gcggtgatta gcttcattat tgatctggtc ggccgcagag ggcaccggta aaaggccatc
     1981 actatttaca aagtaaacca gatatttatg aacagcttga tctcaccact cccctcttgt
     2041 agccttacat gatgcacagc tcacaagagg ggaaaaatgc gttcgttctt cacattattt
     2101 attctttcaa tttcattctt tgctcaagca caaactgtgg agaaaatcac aggcaatgaa
     2161 gccctagtga cggttctagc ctctgaaagc ctcacagttg gcgataaagt aaactttctt
     2221 aacgaaaatt taaacatctc aggacagggt gaggttacca agctttccag tggtggaaaa
     2281 aaagccatcg ttaaaatcat ctcaggaaat gcaaaaccag ggatgagcct cgaaacactt
     2341 tcagccactc caagaccaaa caaaagcttg atggtagata ccttggacga agaagaccgc
     2401 cgaatcctcc acattggtga aatcagtacc tcaagctatg tcatcggagg aatcctcggg
     2461 acttatcctt tagggctagg tatcggtcat gccattcaag gtcgctatat ggataaaggc
     2521 tggattttta ctgtaggaga gttaggatcc cttgccgtgg taatgggggg cgttgggagt
     2581 tgtttggatt ctatatccaa ctccagtcgc tgtaatggcg gagcctggtt tctaggtgcc
     2641 atgggctatg tggggttccg tatatgggaa atcatcgatg tctgggcggc acctccagaa
     2701 atcaaccgac gctatcataa tttgaaattt cgcatggaat ctcgggctat tctgagccca
     2761 ctgctagcac ccaccagaga tggggctgtg tttggtctta ggatgacctt ttaaacgaag
     2821 tgggaacatg gttttgctat aagccggatc ctgtccaaga aactaggtcc caaaaatgtc
     2881 ccaatttcct tagatgatca ttcctctagg agtgccgtta ccgacaccct caagcgatct
     2941 tacccgaaag ctacccacgg gtcgtggtac tagtgctttc ctatttgatc ttgctccgcg
     3001 cagagtttgg ctgttttcac tccggcagct cccctgatcc tcccgttccc ggtgtcaggc
     3061 tcatagcccc ggacattctc tctgttccac tgttccttgc attactgcag aggggcgtta
     3121 ccccttgcgc taccctatgg agtccggact ttcctccccc gcaaaaacgg cggcgatcac
     3181 ccgcaaaacc ctgtttcttg taacaccctt agccataaag agtaaatagg gaagtgaaat
     3241 tttgagtacc caccagaaga tttttccatt aatttgtggt tcttaagtaa aaaggatgcc
     3301 gatatgaaga tttttgtttc cgttcttgtt ttcgtgacag ctttaacagt gggagttcag
     3361 tctcttgcaa tgaataaaga gggctctatt gatatggatg gtctttttcc tcctcctcaa
     3421 agcaatttga gctttggcga acgtccagcc aaacctgaac taaccgctcc cagttttttc
     3481 cagaaaatta acgctagcca ggccacattg acttggaaag ccgtcccgac ggctgagtct
     3541 tatcatgtgc agctggctac tgatcccaac ttcaaatggc tcgttaaaga agagcaactt
     3601 ttaaaaggga ccactttaga agtcgctgga ctagaaagtg gaaaacacta cttctggcga
     3661 gtttccgcta tgaaaccgga aaataaaaac ggccatattc aaggtgcctt tagttcttcg
     3721 atgtttgtga ctagcgcaac taagtaattt tccgtatcat ggttgttcaa cttaaaggac
     3781 aaccatgaac tggctcattt caatattcat tatttttaat gttcataccc tcgctcaggc
     3841 ggctgatgct ttcgttgatc tctatacaaa tagtgttcag cctctcttta atagtcggtg
     3901 tattgcctgc catagttgcc ttaactctcc ttgccagctg aacctgcaaa gttatgaagg
     3961 cttccaacgc ggagctaata aagtgaatat ttataacggc aaccatattg atgccgtgtc
     4021 ccctactcgt ttatggattg atgcccatac tccacaggag tggcaaagta gaggcttctt
     4081 ctccgtaaat accagtaaag aacctgcgaa taatttattc tttcaggcgg tcgcaatcaa
     4141 ggcctcggaa aagtcagcgc ctacaaaaac cgtctttgaa agtcagtctt gtcccgcgac
     4201 ccctactgaa atcaaagtgg ccatgcctta tgctcttccg ggccttaaag ttgaagagtt
     4261 aaaaattttg aaaaagtgga ttcaagaagg agcccccggc cccaatgccg aagcccagaa
     4321 aaccctgtcc tttgttgctc ctgaaattaa aaaacaaatc caaagctggg aaaaattcct
     4381 caatgaaaac gatcttcgcc ataaactcgt gagtcgttat ctttatgaac atcttttctt
     4441 agcccatatt tatttcccgc aggaaccaca aacatttttc cgtctagttc gttccagcag
     4501 taagtgtggc aaaaccatcg acgaaatcgc cacgcgccgc ccgaatgaca atccgggaac
     4561 ggaaagcttt caatactgct ttaaaaaatt cgtcggaacc gtggtgtcta agacccatat
     4621 gccttacgaa ctaagcccgg caaaaaaaga acgttttgag aatctctttt ttaaaacgca
     4681 ctggaaggtc acacgcctcc cgacttataa ttatcaagag gcagagaatc catttttagc
     4741 attcgcggat attcccccaa agtctcgcta tcagttccta ctcgatgacg ctcaatacga
     4801 agtcagtact tttattaaag ggcccgtctg caacggcagc gtggctgtaa actcgattca
     4861 agaacagttt tttatgtttt ttatcaatcc tgaatccgaa atcatgactc gggcacgagg
     4921 atttcaagat caagcgcagc cgctgctgat ccttcctggg gtgtggggaa gtgacgttaa
     4981 actcggagac actccaagtt ttttgactaa actgactgag catcgtgaaa aatttcgatc
     5041 acttcgagcc cgctggaaga aagaactctt tcccaagggc tacgccctcg ctgatatttg
     5101 ggacggggat ggtgaaaatc caaatgctgt tttgaccatt cttcgccatg acgacaatgc
     5161 cgcagtcatg acaggagcta taggcgacct ttctaaaaca attgtcatgc tggactatgc
     5221 cctatttgag cgattggttt acaacctcgt cgtgaacttc gatgtctttg gtaacatcag
     5281 ccaccaatta ctgacccggg attatatgga tatgcttcgc atggaagctg aagagatttt
     5341 cctatccttt cttcctcctg aggagcggct gcccacgagg gattcgtggt acaaaggctt
     5401 cgccgctgaa ctgaaaatga aatatatgta tcctgttctt gagaaggatc tccccacgtc
     5461 tgtggtctat acttctccga aaaacagcaa aaaagaattt gtcgaaaaag tactcttcgc
     5521 gcggatgaat gaaaaagttc gcggagcaat ggattttatt aattggaaaa atattgacat
     5581 tccggagaac gtcttaactc agacagaaaa agcacagaga gaaactattc ccatagactc
     5641 tgaactgcga aaaatcgccg ccgtagctgc tcaagataaa acccctttcg ctcaattttt
     5701 cccagatgtt gcgcttctgc aggtgaaagg aaaaatgggg gagcaaaagg tctacagttt
     5761 gatccataat aaagaacacc aaaatatttc atggattcta gctgaatcca tgagaatgtc
     5821 tccagaagag gattccttgg ttattcgcga aggttattgg ggatcttatc cgaatatgtt
     5881 ctttactgtc agtgaggaac aaatgtccgc attcacgtca caggtcctca aactgaagtc
     5941 tgcgaaggat tatgccaaac tggttcgaaa cttcggcgtc cccagaactc aggagcgctt
     6001 ttggagtgtc tatgacgaaa tcaacgcaca ttttagaaaa actgcaccca cacagttcgg
     6061 atatttggat atcacccggt atgaaatgta aagagtccta aagttttttt aagatttctt
     6121 cccgcttctg attgtattca ccttcagata aaatgccttt ttgatagagt tcgtaaaggt
     6181 tcgctaaatc tgcaggagtt acatcttgcc cggcaccttc ctggggcccc aaggactcat
     6241 ttgactttgc cggaacctta tctaacatac ccaaaacctg aaggacctga tgataccaag
     6301 cttggaaata gttcacattt tttacttcac cccgaacaac taaggtctct gccgccaaat
     6361 catggagagt ctgctttttt tctgtaaaaa gcatcatcaa gaaaccaaag cacaaaatca
     6421 ggccacttac aatgcgaaca aaataacgga tgatcgcttt tttaaaagtg attctctctc
     6481 ctgacgtgga cacaatacgc atatcaagaa gggctttgcc tggagtcgcc tgaagctctg
     6541 aagattcaaa gaagggagtg tataagatag ccaataagat atcgatgccc atggaattca
     6601 gggcaaccat tttaaatagc aaagagatta aaccgacgat aataccatca atgatcacgg
     6661 caccagcgcg tctccaaaat ccagcatagt ccaccgctcc ataacccgac attgtataat
     6721 tcatagatca ctccttaaat gaagctcttt ttaaaccaaa gtttctattc cgagtccaga
     6781 cgaatccact ttaccacctg ctctaaaacg ctgttctgag aggatttatc ttcttccaag
     6841 tagtgaattt ccagcgatgc cgggtcttca ctccagtcat agatcatgcc tgcacacatc
     6901 ttacacggct tgcgagtggt ataaattttt gtacctactg gcagcttctt tcgatgatcg
     6961 tgataaaacc tatgcactgt atttacttct gcatgtaagg ttttattttt ggaattcgaa
     7021 ttcacaccgt aagccaacat attccctgag tgatccgtaa gcagacaagc aatatcgcgg
     7081 tcgtaatcat gtaagacctg acctcgaata ttgagtctcg caatttttcg aatccaggcc
     7141 agcttggagg cct
//
LOCUS       DATBTE010000363        12887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1277915, whole genome shotgun sequence.
ACCESSION   DATBTE010000363 DATBTE010000000
VERSION     DATBTE010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 12887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12887
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277915"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1374
                     /locus_tag="VIG33_13575"
     CDS             <1..1374
                     /locus_tag="VIG33_13575"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEY8271914.1"
                     /translation="AQSVSNLADVFPEQVLKALKMFQDQLPALPPEQAQQILKEELGV
                     DPQTIFRDLDFTKPISTGSIAVLYEAKVQNIFGHWKDVVVKVQRPGLKETLDYNRRLN
                     RLIIQFIKISGGQVAPLMDFIGDQVIGLEDAFEGELDFKKELRNIQRFSHWFRFNKNV
                     QIPSVYPKYSTKKVLVMEKAEGSNLEKNLKALIVRNQQNPSAESTEKKSLESTYVTIF
                     ENMVYMLMVTKELHADLHPGNILAVLEEEENTIRLNKKIVMLDYGQTTKTKGFIWVPL
                     MAGYNLLTGNAEGLAKRLIQMGKDPDFPPDLLIEAIKKIFAENRIEQVKMSSVIRKKS
                     NLNKVESMSKALGQIVKVAFQDLGYRSNPRYFQVLRSSLPVVSTLLLIGKNFTPAEVG
                     KLSLSAFARGLTWGSIYYLSSIPSIAMGELKTNFIEALDKYKNRNSLQSQGQGYSSLK
                     CEAAFAN"
     gene            complement(1455..2141)
                     /locus_tag="VIG33_13580"
     CDS             complement(1455..2141)
                     /locus_tag="VIG33_13580"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY8271915.1"
                     /translation="MDHPQPVVFVLRETLLSRKPDPLAAFAMALATLLVVLISTASRV
                     DFAGVDSWMRASREAVFVHHQYWRAWTSLFVHGDAKHLLSNVFLFFILGSFLTGYFGI
                     CRVLLWALVFGGFINLLVLPSLPLEVDLIGLSGVVFWMGGAWLILYFLIDRKRSLVQR
                     SLRVMGIGLALFMPAEAFDPSISYESHLVGFLLGILAGLLYFSFERKKLRGAEVYEIV
                     RTEDENSPDF"
     gene            2330..3607
                     /locus_tag="VIG33_13585"
     CDS             2330..3607
                     /locus_tag="VIG33_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271916.1"
                     /translation="MYAGKTISFRKLLPILVIGLTGCLDTAQTEDSRATASNCDSQGR
                     SGEKNPHCQQPTPTPKPVEPSWQTVDDVSSYSAGASARGIVKDLAGNLFVSGFGTDDF
                     SHWLVRTNKTGTWQTADDFIYSSGMNSPSFGMGMDLQGNLYSFGGTATGSGPSERWLT
                     RKSVDGVQWTTIDDFQFSNYSVAKDFATSPSGDIFVVGSGFSGGTQHWLVRKSSDHGI
                     TWTIVDDFVYATGQPSYASKVKVDNSGNIYVVGYGQITSSSDFHWIVRKSVNSGSTWS
                     TVDDFQMISGEQSYPFAIEVDGSGNVYIAGEGFDGASPHWIVRKSIDLGSTWSTIDNY
                     QYESRLAYANSLAINQLGVIYVAGVGYDGSAYHWLTRQSTDGGTSWTNIDDVYGARAN
                     SVGIDFDGHPVVAGETDLNNGSPSHWLVRKYIQ"
     gene            complement(3688..3978)
                     /locus_tag="VIG33_13590"
     CDS             complement(3688..3978)
                     /locus_tag="VIG33_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271917.1"
                     /translation="MASVGKANSSSNEIQTLKSSYEMREAQKDHAHEEQIKKINASHQ
                     EEINRVVSESKEGMDKVRERSQNKFTDLEQKYQKEIENLKAMYLKKLAERES"
     gene            4331..6619
                     /locus_tag="VIG33_13595"
     CDS             4331..6619
                     /locus_tag="VIG33_13595"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY8271918.1"
                     /translation="MPPLSGQEIHQMVQRRRVREIEGYTPEKSLSYSFVKESYEFVVS
                     GRADGFVDKPAFIEEIKSTFDIEELQFKLISNSEHPYLWQLRTYGYFHYKLTSELPRL
                     SLNLVSSRNFRDIDIKIELNVEEYERWLEHRLEKLLKETKAREKIFSKRRKMAEKMTF
                     PFPTPRRGQKELVESIEEHFIAQNPLLVQAPTGLGKTAGILYPSLKEALARGQKVIYV
                     TPKNSQHQVAEEAVEKIRDQGCKIKSLTMTAKSKLCLKAEPLCNPKYCEYARDYYKKI
                     EEHDLVNKLSKIRSLSRKKLKSIGEEFQVCPFELSIEAIERAEVVIADYNYVFAPRGL
                     LGRLSEGLLEPKQKPNLVIDEAHNLPSRSQDYFSPSISTQQLTLFERFFPKLPRTYAI
                     QAQALTARAQRLIQSYGKEGVSKIIKIDQEPFAELEQSIRELTMQYLDSDTEIQTQDP
                     LLRLMNMWSEFVNSLNLSGEEFFQTYQRNGFTEMLKITCCDASEHLKLAYKEFKNVVA
                     FSATLKPFAYYQELLGFPNKNTRHVEFSSPFSKDNRKILIIPQISTKYSERVLNAGKI
                     AETINRITQLKRGNYIALFPSFDFMRMVESLLNIPDCEILIQEREMKQIKTEEYLEKL
                     RGVAPTLLLGVQGGVFSEGVDFPGNMLIGAFVVGPALPHFDFEREQIRAYYENRYGKE
                     NAFNYAYVFPAMAKAIQSAGRVIRSETDRGVIIMMDSRFLQNTYSVTMPEGWFEESPQ
                     ELVSNQILQDLKQFWEEVPCES"
     gene            6609..8243
                     /locus_tag="VIG33_13600"
     CDS             6609..8243
                     /locus_tag="VIG33_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEY8271919.1"
                     /translation="MSLKTRNVLFLDLQTTGAKPGSAHIIEIAWSSLFSEIAEAHLVQ
                     LPEGQAIPRQIQYLTGIHEQNMEQAKALPEILFSLKEYLEVHWRPHEEVLAIIHFAQF
                     EKPFLLDAYEQIQQEQLPFSILCTHEIAKRLMPNLPTRGIKGLAGYFGFPSGELKRAA
                     NHVQATKIIWRGLTSTLAEKSIHTLEELKEWLHATPKVARVKYEYPLPREKRLGLPKQ
                     PGIYRMLSKWGEVLYVGKATNLHDRVNSYFRGQKNRDSRKLEMLTQVWDLHVTPVGSP
                     LEAALLETDEIKRLDPPYNICLKSGHRSMAFFNHDFTSFNEEYDQEHKIGPFSNSLVL
                     DSMLRLSHSLQTGDFDQNIFFDPLDSALLKEGFEVFCSRHHLKTEAFRSVRAILAAGL
                     NWVRKSKFEMDMELEANEEIDDIATETANDSAEENPVLTIDDIADKFERHFIRAAKAY
                     LRTKKLNQLLNSDICFKLKNKDSSEKVLKIRQGQVVTNEENDSPILASWKNLSIDTYD
                     RMTVLFTELEKLRSQKHSLRIKNLKSEGLSADTKSL"
     gene            8363..9112
                     /locus_tag="VIG33_13605"
     CDS             8363..9112
                     /locus_tag="VIG33_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8271920.1"
                     /translation="MSSVVSFINQKGGVAKTTTAINVSAQWAQLGKKILLIDLDPQAS
                     ATRAIFGDTEFENTIYDVLTSEIEAKEAVAHSESFGIDVIPSEIMLSGIEIILASKFG
                     RETILKRRLAEIKDDYDIVVIDCSPSLGLLTVNALIASKDIVIPICPEYFSLKGIELI
                     LETLKNIHTGLGHKVDVRGIIISKYRNRRIVEQVIRDLRAKHTIPVFNNYIPDSIVVE
                     EAHHKHLPMHEFSPKNPAGLALANLAMEMWP"
     gene            9109..9603
                     /locus_tag="VIG33_13610"
     CDS             9109..9603
                     /locus_tag="VIG33_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271921.1"
                     /translation="MSNLNPLFLDSTKPIGDKVVTNVELINILLHDDDSLYFEVIDQF
                     GFTQRFALQPIATKKSIKYRAEVWLKYQHQIQYRFVIDSEGQTLFKSAIRETRAGHVI
                     SENWEPCFDGDLVTEKTDKLSIRRAEFMRDLKNPLEIKSNSSQPLCKPQFLEQIKSLL
                     EDLL"
     gene            complement(9650..10393)
                     /locus_tag="VIG33_13615"
     CDS             complement(9650..10393)
                     /locus_tag="VIG33_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271922.1"
                     /translation="MVKDKVLILFLIVTSTVSFAKPSAKVNPGDPCGILASEYKKLKI
                     ASSIQFIARCQKVQNGNFKNEASETLTEGSVCIAKFENPNTLSELPGACDPSETPARA
                     DFYILSGNGHFLTEKGEKRSRVLPSMMKVDLQSLNKMGYYAYTFLKQDRSAESNYINW
                     CMKTIRDEKFDCEKSLLGSPFSFLKLQSGACNSENHIIVKKEGPDQYEALYDYDRSLN
                     ILFIRETVPGDYLLTRSQISAVLNCEEVQ"
     gene            10651..10974
                     /locus_tag="VIG33_13620"
     CDS             10651..10974
                     /locus_tag="VIG33_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271923.1"
                     /translation="MKNLKMAVFSVYTLAALPQMASAHTCAERLYATGRIFAYSAQKA
                     CAGLTEAQANCVADVYTTGLILAHSARKTCRDLTDAQANCVAEVYSQGGQTAHYSRAF
                     CENHY"
     gene            11082..11507
                     /locus_tag="VIG33_13625"
     CDS             11082..11507
                     /locus_tag="VIG33_13625"
                     /inference="COORDINATES: protein motif:HMM:NF016356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEY8271924.1"
                     /translation="MVDAKPKYLISACLVGCNCRYDGKNQRRQEMVDLYKTGEILALC
                     PEELAKLGTPRPACEKNGEMVVTATGLDKSAAYALGAEQALTLAKQQQIQRAYLKSKS
                     PMCGFGTIYDGSFSGTLKVGNGVLAELLLANGFEIESID"
     gene            complement(11691..12839)
                     /locus_tag="VIG33_13630"
     CDS             complement(11691..12839)
                     /locus_tag="VIG33_13630"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY8271925.1"
                     /translation="MQIAILGAGIGGLTMAHCLEKANIPFSLFERAPDIHPVGAGIWM
                     PPNAMNIFKTLDLDSVILESGVPLQKMNLLTKSGSSFSIVDGNKLKSQFGYSAVSLKR
                     YELHKKLYQSLPQHRIHLDHQCTHVEVNSRGVDLFFSNGDQFKADVLIAADGIKSMVR
                     KAIGDHRELIYSGQTCLRAITTLSIPDKIKTEAAEVWGHGLRFGCSPVGPSEVYWYAT
                     LVHPPFTAMSSSKAFKILQNRFRDFPDFVQLIIAHIAPDTIIQTDIFELPEGKRWSKD
                     RIVLLGDAAHSMTPNLGQGAAMAIEDAFVLAKHISNSNTTSLATSLLRYQEQRMQRVH
                     QIAKIARQLGRLGGWQNPFLCRFRNWALKKAPRSLQEKQLEFIFSGPY"
BASE COUNT         3870 a         2802 c         2688 g         3527 t
ORIGIN      
        1 gctcagtccg tgagtaatct ggcagacgtt ttcccagaac aagttttaaa agcccttaaa
       61 atgtttcagg atcagcttcc cgcccttccc cccgagcagg cacagcaaat cctaaaggaa
      121 gagctgggcg ttgatccgca gacaatcttt agagaccttg attttacaaa gccaatctcc
      181 accggatcca ttgcggtttt atatgaagca aaagtacaga atattttcgg tcattggaaa
      241 gacgttgtgg taaaagtaca aagaccgggt cttaaagaga cactggatta taaccgtcgc
      301 ttaaaccgtt taattattca gtttataaaa ataagcggtg gccaagtggc acctttgatg
      361 gattttattg gtgatcaagt tatcggcctg gaagatgctt tcgagggaga gcttgatttt
      421 aaaaaagagt tgcgcaacat ccaaagattt tcccactggt tccgtttcaa taaaaatgtc
      481 caaatccctt ccgtatatcc caagtactcc accaagaaag tattggttat ggaaaaggca
      541 gagggttcta acttggaaaa aaacctcaaa gccctgatag tgcgtaatca acagaatcca
      601 tcagcagaat ccaccgagaa gaaatccctg gaaagtactt atgtgacaat atttgaaaac
      661 atggtctata tgcttatggt caccaaagaa ctgcatgcag atcttcaccc cggaaatatt
      721 ttagctgtat tggaggaaga ggaaaacaca attcgactca ataaaaaaat cgtgatgcta
      781 gactatggcc agacaacgaa gacgaagggt tttatctggg tgccactcat ggcgggatat
      841 aatcttttaa cgggtaatgc cgagggcctt gccaaaagat taattcaaat gggaaaagat
      901 ccagattttc cgccagactt actgattgaa gctattaaga aaatttttgc cgagaatcga
      961 attgaacagg taaaaatgag ttcagtgatt cgtaaaaagt ccaaccttaa taaggttgag
     1021 agtatgtcca aggcacttgg acaaattgtt aaagtggctt ttcaagactt gggctacaga
     1081 agcaatccac gttattttca agtgcttcgt tcctccctgc ctgtcgttag cactcttctc
     1141 ctgattggaa agaacttcac gcctgcagaa gttggcaaat tgtcgctgag tgcatttgcg
     1201 cgtggtctaa catggggctc tatctattac ttatcgtcca ttccgtccat tgccatggga
     1261 gaattgaaaa ccaattttat tgaagccctt gataaataca aaaatagaaa ctcgttgcag
     1321 tcacaaggtc aaggttatag ttcccttaaa tgtgaagctg cgtttgcaaa ttaatatttt
     1381 atttgccata aacttaatca gacccatgag caaatcttta tcctgtgact tgacaatgta
     1441 aacttaataa cggtttaaaa gtcagggcta ttttcgtcct ctgtcctcac gatctcataa
     1501 acctccgcac cacgaagttt ttttcgttca aaggaaaaat acaaaagccc ggccaggatt
     1561 cccaacaaaa accccaccag gtgactctca tagctaatgg aagggtcaaa agcttcggca
     1621 ggcataaaaa gagccagccc tatccccatg actcttaaag atctctgaac cagactgcgt
     1681 tttcgatcaa tgagaaaata aagaatcagc caagcgcccc ccatccaaaa aacgactccc
     1741 gaaaggccga tcaagtccac ttccaacggc agcgagggca gcactaaaag attgataaat
     1801 ccgccaaaga ccagggccca aagtaagacc ctgcaaatcc caaaatatcc cgtcagaaaa
     1861 cttcccagaa taaagaataa aaaaacattg ctcaataagt gtttcgcatc gccatgaacg
     1921 aagagactcg tccaagcccg ccaatattga tgatgaacaa aaacggcttc gcgactggcc
     1981 ctcatccacg aatcgacgcc cgcgaagtcc acacgagaag ctgtgcttat tagcaccacc
     2041 aacaaagtgg ctaacgccat ggcgaaagcc gccaagggat ctggcttccg actgagaaga
     2101 gtctcacgaa gaacaaatac taccggctga ggatgatcca caaggcctcc tgaaccgata
     2161 gcatgtgtct ctactgaggc ttgtcaaggc ggtatatcca gaattgcaaa gcgagcttgc
     2221 tatataacta aatccctcat ttacctcaag cttaagttaa ttcctgttta taccgaaaag
     2281 cctatgtaag caagaaggca cacgtctcat taggagacag gaggtctgta tgtacgctgg
     2341 caagactata tcttttcgaa agttactgcc gattctagtt attggtttaa cgggttgcct
     2401 tgatacggca caaacagaag attcaagggc tacagcatcc aactgcgact cgcagggacg
     2461 aagtggtgag aagaatcctc actgccaaca gccgactccg actcctaaac cagttgagcc
     2521 gagctggcaa accgtggatg atgtttcatc gtacagtgct ggcgccagtg ctcgaggaat
     2581 cgtaaaagat ctcgccggaa acctatttgt ttctggattt ggcaccgacg atttctcaca
     2641 ctggcttgtt cgaacaaata aaacaggaac gtggcagact gctgacgatt ttatttattc
     2701 aagcggtatg aattcaccat ccttcggcat gggcatggat ttacaaggaa atttatattc
     2761 atttggagga accgcaacgg gcagtgggcc atctgagaga tggttgacgc gaaagagtgt
     2821 cgatggagtt caatggacaa ctattgatga ttttcaattt agcaattatt ctgtagcaaa
     2881 ggatttcgcc acatcaccct ctggtgacat atttgttgtt ggaagtggtt tttctggtgg
     2941 aacgcagcat tggcttgtcc gaaaaagttc ggatcacggg attacctgga caattgtaga
     3001 tgattttgta tacgccactg gacagccttc ctatgcctca aaagttaagg tagataattc
     3061 aggtaacatt tatgtggttg gatatggtca gatcacaagc agttcagact ttcattggat
     3121 agtccgtaaa agtgtaaata gcggttctac atggtccacc gtggacgact ttcaaatgat
     3181 atcaggcgaa cagtcatacc cctttgcaat tgaagtagac ggaagtggga atgtatatat
     3241 tgccggcgaa ggatttgatg gggcttcccc tcattggatc gttagaaagt cgattgacct
     3301 aggaagtacc tggagtacaa ttgacaatta ccaatatgag tcccgcctcg catatgcaaa
     3361 ttcattagca attaatcaat tgggagtcat ttatgttgct ggagtaggtt acgatggcag
     3421 tgcctaccat tggcttacac gacagagtac tgacggaggg acatcgtgga ccaatatcga
     3481 tgacgtgtac ggtgctcgcg caaatagcgt aggcattgat ttcgatggcc atcccgtcgt
     3541 ggcaggtgaa acagacctaa acaatggatc accctctcac tggcttgtcc gaaaatacat
     3601 tcaataatcg cctgagaagg ggcttccgca agaatatgaa gttcggcgcg aaaacaccgt
     3661 ggtagcgatt taatatctaa aacactatta gctctctcgc tcagctagct ttttcaggta
     3721 catggctttg aggttctcga tttctttttg gtacttttgt tctagatccg tgaatttatt
     3781 ctgacttcgc tcgcgcactt tatccatgcc ttctttcgat tcgctcacaa ccctattgat
     3841 ttcttcctgg tggctagcat ttattttttt tatttgctcc tcgtgcgcat gatctttctg
     3901 agcttcgcgc atttcataac tgcttttcag cgtttgtatt tcattggaag aggaattcgc
     3961 ttttcctacg gatgccaccg ataacctcgc tggttgatag atcaatgata aaatatttcg
     4021 cttccgcatt gtaaccgaag cacaaaatct agacgaaggc cccaaggaag ttgggatata
     4081 ccctgcccaa ttgcaatgct tgacactcaa ttagaccttt tctcggtaat gttacatttt
     4141 ccaaacaaat aaagataaga cgcaaccaag atttgatctc cgtcttgcaa gtatgaaagg
     4201 ttgaaataac tctgggaata acttaaaaag gtcgcaatcc aaaattatgc gtaaaatcca
     4261 tctcgatgtc agacaatttt ctgtcccttg tcccatggta gggaatatag aaacccattc
     4321 gggttacggt atgccaccac tcagcggcca agaaatccac cagatggtac aaaggcgaag
     4381 agttcgtgag atagaaggat atacacctga aaaaagtctc agctattctt ttgttaaaga
     4441 atcttatgag tttgttgttt cagggcgggc tgatggcttt gtagacaagc ctgcgtttat
     4501 tgaggaaatc aaatccactt ttgatattga ggagcttcaa ttcaaactta tttccaactc
     4561 cgagcatccc tatttgtggc aattacgtac ctatggttat tttcactata aactcacgag
     4621 cgaactcccc cgtttaagcc tgaatttggt ttcttccaga aactttcggg atatcgacat
     4681 taaaatagaa ttgaatgtgg aagaatatga aagatggctg gaacatcgtc tcgaaaagtt
     4741 gctgaaggaa accaaagcta gagaaaagat attcagtaaa agacggaaaa tggctgagaa
     4801 gatgactttt ccatttccaa ctccacgacg tggtcaaaaa gagttggtgg aaagcatcga
     4861 agaacatttc attgcacaaa atccactttt agttcaagcc cccacaggcc ttggcaaaac
     4921 agcgggtatt ttatatccct ccttaaaaga ggcattagct cgtggacaaa aagtgattta
     4981 tgtgacacct aaaaattccc aacaccaagt agcagaagag gccgttgaga aaatcagaga
     5041 tcaaggttgt aaaatcaaat ccttaactat gaccgcaaaa agtaaactgt gcctaaaggc
     5101 cgaacctctg tgcaatccga aatattgtga gtacgcgaga gattactata aaaaaattga
     5161 ggaacatgat ctcgtcaata agttaagtaa aatacgcagc ctcagtcgaa aaaaactaaa
     5221 atccatagga gaggaatttc aagtttgtcc tttcgagtta tccattgaag ccattgaacg
     5281 cgccgaagtg gtgatcgcag attacaatta tgtttttgct ccccgtggat tattaggccg
     5341 actttctgag ggtttgcttg aaccaaaaca aaagccgaat ttagtcattg atgaagccca
     5401 taatctcccc tctcgttcac aagactattt ttctccgagt atttcaacac agcaattgac
     5461 tttgtttgag agatttttcc ctaaacttcc ccgcacctat gccattcaag ctcaagcatt
     5521 gactgcccga gctcaacgcc ttattcaatc ttacggcaag gagggggtct ctaaaataat
     5581 taaaattgac caggaaccct ttgcggaact ggagcaatcg attcgtgaat tgaccatgca
     5641 atatcttgat tccgatacgg aaattcaaac tcaggaccct cttttgagat taatgaatat
     5701 gtggtcggaa ttcgtaaatt cgttaaacct cagcggggag gagtttttcc aaacttatca
     5761 aagaaacggt ttcactgaaa tgctgaagat tacctgctgt gacgcttccg agcatttaaa
     5821 actggcctac aaagaattta aaaatgtagt cgctttttct gccaccttaa agccttttgc
     5881 ttattaccag gaactcttag gttttccgaa taagaacacc cgacacgttg agttttcctc
     5941 ccctttttct aaggacaatc gtaaaatttt aattattccg caaatttcaa ccaagtattc
     6001 agagcgtgtt ttgaatgccg gaaagattgc tgaaaccatt aaccgcatta cgcaactcaa
     6061 aagaggaaat tatattgctc tttttcccag ctttgatttt atgcgtatgg tagaaagtct
     6121 cttgaacatt ccagattgcg aaattctgat tcaggagcgc gaaatgaaac agattaaaac
     6181 tgaagagtac ttggaaaaat taagaggtgt cgcgcccacc ttgcttttag gggtgcaagg
     6241 aggcgttttt tcagagggtg tggattttcc gggtaatatg ttaatcggcg ccttcgtggt
     6301 tggccctgcc ttacctcatt ttgattttga acgtgaacag atccgcgctt attatgaaaa
     6361 tcgttatggt aaagaaaacg cttttaatta tgcctacgtt tttcccgcaa tggcaaaggc
     6421 gattcaatct gccggaagag tcattcgctc tgaaacagac cgaggcgtaa ttataatgat
     6481 ggattcacgt tttctacaaa acacctattc agtgacaatg cccgaaggct ggtttgaaga
     6541 atctcctcag gagcttgtat caaaccagat tcttcaggac cttaagcaat tctgggaaga
     6601 ggtcccgtgt gagtcttaaa acaaggaatg tattattttt ggatttacag accacaggag
     6661 cgaagccggg ctcggcgcat attattgaaa tagcctggag ctctttattt tcagaaatag
     6721 ctgaagctca ccttgtacaa cttcctgaag ggcaagccat tccgcgccag atccaatatc
     6781 tcactggcat tcatgaacaa aatatggaac aggccaaagc actgccggag attttatttt
     6841 cactcaaaga ataccttgaa gtccattggc gcccccacga agaagtttta gcaattatac
     6901 acttcgccca gtttgaaaag ccttttttgc ttgatgccta tgagcagatt caacaagagc
     6961 aactgccttt ttcaattctc tgcactcacg aaattgcaaa acgtctgatg ccaaacttac
     7021 ccactcgggg catcaaggga ttggcgggat actttggttt tccatcgggc gagctgaagc
     7081 gagccgctaa tcatgttcaa gccactaaga tcatttggcg gggtctgacc tcaactttag
     7141 cagagaaaag tatccatacc cttgaagaac tcaaagagtg gctgcatgca acacctaaag
     7201 tggcacgggt taagtatgaa taccccttac ccagggaaaa acgtttaggt ctgccaaagc
     7261 aacccggtat ttaccggatg ctcagcaaat ggggtgaagt tctttatgtg ggtaaagcca
     7321 ccaaccttca tgatcgtgta aacagttatt ttcggggcca aaaaaaccgc gattcaagga
     7381 aactggaaat gctcactcaa gtttgggatt tacacgtaac tccagtagga agtcctttgg
     7441 aagcagccct gcttgaaaca gatgagatca aacgccttga tccaccctac aacatatgct
     7501 tgaaatctgg ccatcgcagc atggcgtttt tcaaccatga cttcacctcc ttcaatgaag
     7561 aatatgacca agaacataaa atagggcctt tttcgaattc tttggtattg gactcgatgc
     7621 tgcggctgag ccactccctt caaaccgggg atttcgatca aaatatcttt tttgatccct
     7681 tggactcggc acttttaaaa gaaggatttg aagtcttctg ctcacgtcat catttaaaga
     7741 ctgaagcttt ccgttcagta agagctattt tagccgcggg attaaattgg gtaagaaaga
     7801 gcaagtttga aatggatatg gaattggagg ccaacgaaga aatcgatgac atagctactg
     7861 aaactgcaaa tgattctgcg gaagaaaacc ccgtactgac catcgatgac atcgctgata
     7921 aatttgaaag gcattttatt cgagctgcca aggcttatct tcgaactaaa aagttgaatc
     7981 aacttttgaa ttccgatatt tgttttaagt taaaaaataa agacagctca gaaaaagtct
     8041 taaaaatccg ccaggggcaa gttgttacaa atgaagaaaa cgacagccct atcttagcct
     8101 cctggaaaaa cctttccata gatacctatg atcgcatgac ggtcttattc acagaattag
     8161 aaaagctaag gtcgcaaaaa cattcgctga gaataaaaaa ccttaaatcc gaaggactct
     8221 cagcagatac caaatcccta taaaaaacct agacctttga cctgaaataa ctcaacaacc
     8281 acctcttgct tctcctccaa ggcggaaaag gctagcctaa agtagtgaat gtgaatcatt
     8341 tcttaaagtg agggaaaacc acatgagcag cgttgtttca tttatcaatc aaaaaggtgg
     8401 agtcgccaaa acgacaactg cgatcaatgt atcagcccaa tgggcgcaat tgggaaagaa
     8461 gatcctcctg atcgatctcg atccacaagc ctctgccact cgagcgattt ttggagacac
     8521 cgagtttgaa aataccatct atgatgttct cacctcggaa attgaagcca aagaagccgt
     8581 cgctcactct gagtctttcg gtatcgatgt catcccctct gaaatcatgc tcagtggaat
     8641 cgaaatcatc ctggcttcaa agttcggtcg cgaaaccatt ttaaaaagac gcttggctga
     8701 gattaaagat gattacgaca ttgttgtcat cgactgctcg ccttccttgg gactgcttac
     8761 tgtcaatgcc ctgatcgcct ctaaggacat cgtgattcct atctgcccag agtatttttc
     8821 tttaaagggg attgaactca tattagagac gcttaaaaat attcatacag gactgggaca
     8881 taaagtcgac gtgcgtggga tcattatttc caaataccga aaccgccgca tcgtcgagca
     8941 agttattcgc gaccttcgcg ccaaacacac catccccgtg ttcaataact atatcccaga
     9001 ttccatcgtg gtcgaagagg cacatcataa gcatctacct atgcacgaat tctcgccaaa
     9061 aaatccagca ggccttgctc tggctaatct ggctatggaa atgtggcctt gagtaacctt
     9121 aatcctctat ttttagattc aactaaacca attggcgaca aagttgtcac caatgttgag
     9181 ctcatcaata ttctcttaca cgatgatgac tctctttatt ttgaggtgat tgatcaattc
     9241 ggcttcaccc aaagatttgc attgcagccc attgcaacca agaaatccat aaaatatagg
     9301 gccgaggttt ggctgaagta ccagcatcaa attcaatatc gcttcgtgat tgactccgag
     9361 ggacaaactc tcttcaaatc cgcgattcgc gaaacgcgcg ctggtcatgt catttctgaa
     9421 aactgggagc cctgctttga tggtgacttg gtcacagaaa aaacagacaa gctcagtatt
     9481 cgtcgagctg agttcatgag ggacctcaaa aatcctctgg aaatcaaatc aaattcttcc
     9541 caaccccttt gcaaacccca gttcctggag cagatcaaat ccttgctgga ggatttattg
     9601 taactaacaa tccaaggttc tgctcactta gcgtggcctg gctacacttc tattgaactt
     9661 cttcgcagtt taaaactgct gaaatttggc tgcgagttaa aagatagtct cctggcactg
     9721 tttccctaat aaaaagtatg ttcaaacttc tgtcgtaatc ataaagagct tcatattgat
     9781 ctggcccctc tttttttact ataatatgat tctcggagtt acaagctccg gattgcaatt
     9841 taagaaaact gaaaggactc ccaagcaaag atttctcaca atcaaacttt tcatccctga
     9901 tggttttcat gcaccaatta atatagttgc tttcggcact tctatcttgc tttaggaacg
     9961 tataggcata ataccccatt ttattaaggg attgcagatc aactttcatc attgatggaa
    10021 gtacgcggga gcgcttctcg cccttttccg ttaaaaagtg accgttgcca gaaagaatat
    10081 aaaaatccgc tcgcgctggc gtttctgagg ggtcacaagc tccaggaagc tcacttaaag
    10141 tattcgggtt ctcaaatttt gcgatacaaa ccgaaccttc ggtcaaagtt tcagatgctt
    10201 catttttaaa gttcccattt tgaacctttt ggcaacgagc aataaactga atcgatgaag
    10261 ctattttcag ttttttatat tcgctcgcca agatgccaca aggatcccct ggattcactt
    10321 tcgcagaagg ctttgcaaaa gacacagttg aagttacgat caaaaacaaa atgagaactt
    10381 tatccttaac catttaaaat ctccacatta agagctccat ttatactaaa atttaataat
    10441 tttttgtgaa aaaggtcttt atttgaaaga gtttttagag gtgtctaaaa gttcatcagc
    10501 ggaatttatt aagccactaa ttttcatttc aagtcagatg aagggtcaaa gctacggcct
    10561 agattcacta gatatttttt tcactatcca taaaaatgag ttttaatcga caaagacctt
    10621 gaataagaca taacaaacaa aggagaattt atgaaaaatt taaaaatggc tgtcttttct
    10681 gtgtatacat tggctgcttt accacagatg gcctctgcac acacttgcgc ggaaagactt
    10741 tacgcgactg gaaggatctt cgcttattca gctcagaagg cgtgtgccgg attaacagag
    10801 gcccaggcaa attgcgtggc tgacgtttat accactggct tgatcttagc tcattccgct
    10861 cgcaaaacat gcagagattt aacagacgct caagcaaatt gcgtagctga ggtttactcc
    10921 caaggaggac aaaccgctca ttactcccgt gccttctgcg agaaccatta ctaaatcact
    10981 tagtccttgg cctgcaagaa gccggatttg aaaattctgg cttcttcagt tcgtaacttt
    11041 tttattcact taaatcttaa tttcagatac acccatcgcc gatggtagac gcaaaaccca
    11101 aatatcttat aagtgcttgc ttagttggtt gcaactgtcg ctatgatgga aaaaaccaaa
    11161 gacggcaaga aatggtcgat ctttacaaga ctggggagat tcttgccctt tgccccgagg
    11221 aattggctaa actcggcact ccccgcccag cctgtgaaaa aaacggggaa atggtggtaa
    11281 cagccactgg cttagataaa tcggccgctt acgctctggg agcagagcaa gccctgacct
    11341 tagcaaaaca acaacaaatt caaagggcct atctcaaaag taaatcacca atgtgtggct
    11401 ttggtacaat ttatgatggt tccttttccg gaactctgaa ggttgggaac ggcgtcctcg
    11461 cagagcttct ccttgccaat ggatttgaaa tagaatctat tgattaaaga gcgtttttca
    11521 ggaacaactc gtggatagtt ttattttaaa tatgaatgat ctgacgtagc caggatatga
    11581 tacccgaaat actgtaaggt tttgcgatga agagcccgaa tggcaaaaca ttttacgttt
    11641 aattattaaa gtctaataga gaataactaa ggaaactgaa tctcacgtct ttaataagga
    11701 ccagagaata tgaactcaag ttgtttttct tgcaaagagc gaggggcctt tttcagagcc
    11761 caattgcgga agcgacacaa gaatggattt tgccaccccc cgagtcgccc tagctgcctt
    11821 gctatctttg caatttgatg tactcgctgc attcgttgct cttggtatct taataaagag
    11881 gtcgcaagcg atgtcgtatt agaattggaa atatgctttg ccaggacaaa agcatcttca
    11941 atggccattg cggcaccctg ccccaggttc ggcgtcattg agtgagccgc atccccaagc
    12001 aatacaattc gatctttgct ccagcgcttt ccctcaggca attcgaagat atccgtttga
    12061 atgattgtgt ctggagcaat gtgggcaatt attagctgaa caaaatcagg aaagtctcgg
    12121 aatcgatttt gtagtatttt gaaagcttta cttgatgaca ttgcggtgaa cggtggatgc
    12181 actaaagttg cataccaata aacttcggaa ggacctactg gtgaacaacc aaaacgcaag
    12241 ccatgtcccc agacttcagc cgcttcagtt tttattttgt cgggaattga gagtgtagtg
    12301 atagcacgca aacaggtttg ccctgaatag atgagctcgc gatgatctcc aatagctttg
    12361 cgaaccattg attttattcc gtcggctgca ataagaacat cggccttgaa ttggtcgccg
    12421 ttactgaaaa acaaatcgac tcctcgagaa ttcacttcga catgggtgca ttgatggtct
    12481 agatgaatac ggtgttgagg caaggactga tataactttt tatgaagctc atatcgtttg
    12541 agggatacgg ccgaatatcc aaattgactt ttgagtttat ttccgtcaac gatactaaaa
    12601 ctagatccag atttagtgag tagattcatt ttctgcaagg ggactcctga ttccaagatg
    12661 acagagtcga gatctaaagt tttgaagata ttcatagcat tgggtggcat ccaaattccg
    12721 gctccgacgg gatgaatgtc aggagcacgt tcaaaaagag aaaagggaat gttagccttt
    12781 tctaaacaat gtgccatcgt taatccaccg atacctgcgc ctaggattgc aatttgcata
    12841 aatactccat ttaaatttca gttctatgct ctcataagaa tgtgatt
//
LOCUS       DATBTE010000364         3374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1210463, whole genome shotgun sequence.
ACCESSION   DATBTE010000364 DATBTE010000000
VERSION     DATBTE010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210463"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VIG33_13635"
     CDS             <1..994
                     /locus_tag="VIG33_13635"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092799.1"
                     /note="catalyzes the ATP-dependent carboxylation of a
                     covalently attached biotin and the transfer of the
                     carboxyl group to pyruvate forming oxaloacetate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEY8271926.1"
                     /translation="RVVRKMEELESAYRACRSEGQNYFANPTVYIEKFVNDPKHIEIQ
                     IFGDKHNNHVHLFERECSVQRRNQKIIEECPSPSLTNEVRLKMGAAAVRAAKQINYVG
                     AGTIEFIFDNQTKEFYFMEMNTRLQVEHPITEIVTGYDLVKEQIFVAAGRPLSFKQED
                     IKQKGHAIEARICAEDPVTYKPSPGVIRACRHPQGPFLRVDSYAYPGYEVPIYYDPMI
                     AKVITFGETREEAINRMQRALTEFVLTGIKTNIVLHKTILDHPKFRDGSYTTQFIEKN
                     FEVIEPLLFKEIEDPMFLIAAAITAYNDRKNRDVRQLNITSNWKRTGRKLQLRT"
     gene            998..1528
                     /locus_tag="VIG33_13640"
     CDS             998..1528
                     /locus_tag="VIG33_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEY8271927.1"
                     /translation="MYFEAELQGRKYKVDVTEQRASWKISLQEEGKDWIHYDISKNDY
                     KFAEEYVSFLFEGKSYLIDAIGQDTEYTVFTRNSFRTIKIFNDEMLLHESLKKGGGFG
                     GDVELKAGMPGKVIEIFVKEGEVVKANKSLLIMEAMKMENEMRASRDVKVKEILVKQG
                     DSVETGAVMIKFEEAT"
     gene            complement(1547..2311)
                     /gene="tatC"
                     /locus_tag="VIG33_13645"
     CDS             complement(1547..2311)
                     /gene="tatC"
                     /locus_tag="VIG33_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY8271928.1"
                     /translation="MIETQAQMDEKHQTLVEHLGELRTRVIFCGYALFIATGICYGFS
                     EKIFDFVRQPIAPYLTNGGLIYTAPMDKFIAHLKLSFVCGVIISCPFWLYQIWLFVAP
                     GLYKKEKNYSVGFIMTGTILFLLGVCFSYFVVLPMAFHFLMTYGGEVDKPMITIDHYL
                     GFFTQMCLMFGAAFELPLILIILGMLGIVTQKFLREKRRYAVMILAVISAIITPPDLL
                     SMLMMLGPMIVLYEVAVFFVGFFEKKKTLASVNERE"
     gene            complement(2304..2570)
                     /locus_tag="VIG33_13650"
     CDS             complement(2304..2570)
                     /locus_tag="VIG33_13650"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY8271929.1"
                     /translation="MFGLSLTEIVFLAILALIVIGPKQLPEVARTLGRMLNELRRASS
                     SLTNELRQHVKIDSLPIDEVKAPPPVPSATPETKSEDSEVKNHD"
     gene            complement(2611..3255)
                     /locus_tag="VIG33_13655"
     CDS             complement(2611..3255)
                     /locus_tag="VIG33_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271930.1"
                     /translation="MSQNESKSQITGSDLFISPGDFFKDLVRQGFAQRKLETYPTVET
                     YLVDLLHYYLNAKNLFEPDYDEFGRKIPKTLAEMYFYANNAEDQLIKVELLKKLGDRS
                     LYMSGFFGDSLQRKIVDVDYYVNMGGAAYATLASCVREDTSAKVYSTISKRFIEFVDV
                     LTYISHSSLIKSNESVLRLYDRYMTTGSELAREKLTEMGVLPLAQEQTKRNRQY"
BASE COUNT         1098 a          694 c          674 g          908 t
ORIGIN      
        1 gcgcgttgtc cgtaaaatgg aagaactaga aagtgcctat cgggcctgcc gctctgaggg
       61 acagaactat tttgcaaatc ccacggttta tattgaaaag tttgtgaacg acccgaagca
      121 tatcgagatc caaatatttg gggacaagca caacaaccat gttcatttat tcgaacgtga
      181 atgttcagtt caacggcgaa accaaaagat tattgaagag tgtccctctc cctctcttac
      241 aaatgaagtt cgcttaaaaa tgggggcggc tgcagttcgt gcagctaaac aaatcaacta
      301 tgttggggct ggtaccatcg aattcatctt tgataatcaa actaaagaat tctatttcat
      361 ggagatgaac actcgtctgc aagtcgagca tcccatcact gaaattgtaa ctggttatga
      421 tctggtgaag gaacaaatct tcgttgcggc agggcggcct cttagcttta aacaagaaga
      481 catcaaacaa aaaggtcatg ccatagaggc gcgtatctgc gctgaagatc ccgtgaccta
      541 taagccaagc cctggagtca tccgtgcttg tcgccatccc caaggtccct ttttacgcgt
      601 ggattcctat gcttatcccg gctatgaagt ccctatttat tatgatccca tgattgcgaa
      661 ggtgatcact tttggggaaa ctcgcgagga agccatcaac cgtatgcaaa gagcccttac
      721 tgaatttgtt ttaacaggaa ttaaaacaaa tattgtactt cataaaacta tacttgatca
      781 ccctaaattc cgtgacggat cttataccac tcagttcatt gaaaaaaact ttgaagttat
      841 tgagccgttg ctctttaagg aaatcgaaga tccaatgttt ttgatcgctg cggccatcac
      901 tgcctacaat gatcgtaaaa atagagacgt gcgccaactc aacatcactt ccaattggaa
      961 acgtacgggc cgcaaacttc agttaaggac ataatttatg tatttcgaag cagaactcca
     1021 agggcgcaaa tacaaagtcg atgtaacaga acaaagagcc agctggaaaa tctctcttca
     1081 agaagagggt aaagattgga ttcattatga tatttctaag aatgattaca aattcgctga
     1141 agaatatgta agttttcttt ttgaaggaaa atcatattta atcgatgcga tcggccaaga
     1201 tacagagtac accgtcttta cgcggaactc tttcagaacc atcaagattt tcaatgatga
     1261 aatgcttttg catgagtctt tgaaaaaagg tggcggtttt ggcggagatg tggaactgaa
     1321 agccggtatg ccgggtaaag taatagaaat ttttgtaaaa gagggtgagg ttgttaaagc
     1381 gaacaaatcc ttgctaatta tggaagctat gaaaatggaa aatgaaatgc gcgcctctcg
     1441 cgatgttaag gtaaaggaaa tccttgttaa acaaggagac agtgtggaaa ctggagctgt
     1501 tatgattaaa tttgaggaag ctacctaact aggattggcg ttaattctac tcacgttcat
     1561 tcactgaggc caaagttttt ttcttctcga agaagcctac gaaaaatacc gctacttcgt
     1621 aaagaacaat cattggccct aacatcataa gcatactgag aaggtctggt ggggtaatta
     1681 tcgccgaaat aaccgccaag atcataacag cataacgacg tttttctctc aggaatttct
     1741 gagtcacaat accgagcatt ccaagaataa taagaattaa tggaagctca aaagccgcgc
     1801 caaacattaa acacatttgc gtgaagaagc cgagataatg atcaatggtg atcatcggct
     1861 tatcaacttc tcccccataa gtcataagaa agtgaaaggc cattggcaaa acaacaaagt
     1921 aagaaaaaca gacacctaac aagaacaata tagttcctgt catgataaag cctacgctgt
     1981 agtttttctc ttttttataa agacctggag ccacaaaaag ccagatttga taaagccaaa
     2041 agggacaaga tataattacc ccacaaacaa aagaaagctt taaatgggca ataaatttat
     2101 ccataggagc tgtatagatc aagcccccat ttgttagata gggcgcaatt ggttgacgga
     2161 caaaatcaaa aatcttttcg ctaaaaccgt aacaaattcc tgtagcaata aacaaagcat
     2221 aaccgcaaaa tataacacga gttctcagtt ctccgagatg ctcaacaagt gtttgatgtt
     2281 tttcatccat ttgtgcttga gtttcaatca tgatttttta cttcagagtc ttcggatttt
     2341 gtttcaggag ttgcggaagg aacaggcggc ggtgccttta cctcatcaat agggagagaa
     2401 tctattttca catgttggcg aagctcattc gtcagagaac ttgaagctct gcgcagctca
     2461 tttaacatac ggcccaaagt tctagcaact tccggcaatt gtttaggccc aatcactatc
     2521 aaagctaaga tagctaaaaa tacaatttca gtaagactca aaccaaacat ggattataca
     2581 ttaacgaaat gactccagac agtcacgaaa ttaatattgt cggttccgtt tggtttgctc
     2641 ttgtgccaat ggcaaaacac ccatttcagt caatttttca cgagccagct ctgaaccagt
     2701 cgtcatatac cgatcgtaaa ggcgaagaac gctttcattg cttttaatta aggaactatg
     2761 actgatgtag gtcaagacat caacaaactc tataaagcgc ttcgaaattg tggagtacac
     2821 tttcgcggaa gtgtcttcac gaacacagct agccaacgtg gcgtaagccg caccacccat
     2881 attcacataa taatcaacat ctacaatttt gcgctgaagg gagtccccaa aaaatccact
     2941 catgtataaa gagcgatccc ccagcttttt aagaagctct actttgatga gttgatcttc
     3001 tgcattgttt gcgtagaaat acatctccgc taaagtttta ggaattttac gaccgaactc
     3061 atcataatcc ggctcgaaaa gatttttagc gttcaaatag tagtgcagaa gatcaaccaa
     3121 ataagtttcg acagtggggt aagtttcgag ttttctctgc gcaaaacctt gccgcacaag
     3181 atctttaaaa aaatctcctg gcgaaataaa taggtccgat cctgttattt gcgactttga
     3241 ttcattttga gacaaacaac accctctagg ttattatacc agattctgat gaaaattggt
     3301 caacatctca aaatgaatca gaccttgggc ctagaaggga ttttcaggac ctaccacgct
     3361 aattataaaa tgct
//
LOCUS       DATBTE010000365         5274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2303588, whole genome shotgun sequence.
ACCESSION   DATBTE010000365 DATBTE010000000
VERSION     DATBTE010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2303588"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VIG33_13660"
     CDS             <1..522
                     /locus_tag="VIG33_13660"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEY8271931.1"
                     /translation="KMSATMESYMAGLLYCMPHVLPMYAPTINSFKRLVEGAWAPTTV
                     TWGMDNRTTAVRALPGSSKSTRIELRVVGSDANPYLAHAAALASGLYGIKHKMKLSVP
                     ATTGNGYRDLSNGILPANLWESTQTMKNSKVAAEMFGENFVKHFTGTREWEWRQFAKT
                     VTNWEYKRYFEII"
     gene            540..1697
                     /locus_tag="VIG33_13665"
     CDS             540..1697
                     /locus_tag="VIG33_13665"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEY8271932.1"
                     /translation="MNHDWNKRYNYNFPTLVRFGNGVSEEVGPHLKEQGLKKALVVSD
                     AALIKLAFFENFISTLKKSGLEAVVFSDIAKNPVKSNVMKGVEDYLKNNCDCIIGFGG
                     GASMDVARAIALKAHHTLDLFDYDDAKGGDRFVTGTVPYFVTLPTTSGTGSEVGRSTV
                     ISDDITHEKKILFSPKLMPRKVFADPGLTMGLPAFVTATTGMDALTHNIEAYLSKGFS
                     PLCDGIALEGLRLIGESLERATHNPDLESRSKMMMGALMGATAFQKGLGIVHSLAHPL
                     STVMDMHHGLANAIMLPYGMEFNKSVSGPRMTTMARTLGLKDTSPDGFIAWIRELNKK
                     LKIPARLSEQNVNSSHIEKLAELAINDPCHSCNERPVTKKDFTALYQEAVQ"
     gene            1694..2437
                     /locus_tag="VIG33_13670"
     CDS             1694..2437
                     /locus_tag="VIG33_13670"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY8271933.1"
                     /translation="MIKLGVSACFMHPDPARTVFGQKTLTYLERDMSRFLSRKDVMPI
                     LLPDVAWEQLEPYLKEMDGFVFQGGADISPLNYNESLIENGRWPGDPYRDQYELKIMD
                     WAFKNKKPILAVCRGFQLANVYFGGSLYQDLKVQTKTEVEHRNAELYDKIYHEVECTH
                     GSLLEKIYGQKKLWVNSVHHQGVKVLGKNLVIDAICPKDQLIEAFHYQNMDDHFVLAI
                     QWHPEFSHTIQDKVINPEPLLNYFLERFV"
     gene            2434..3798
                     /locus_tag="VIG33_13675"
     CDS             2434..3798
                     /locus_tag="VIG33_13675"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY8271934.1"
                     /translation="MKIVNPATNALIKELSEDNKATVFEKFAVAQKAQLKWQKLSLDE
                     RLACIKKFESALDQNKENLAKDLTSEVGKPYQESLNEINGARNRIRFFIENSKKWLCE
                     NNVNQDGSTKEVLSFDALGVIANISAWNYPFLVGTNVFIPALIAGNAVLYKPSEYATL
                     TGLNIENLLHESGVPAGVFTAVIGDAKAGEALLDLPLDGYFFTGSYKTGRAIAEKVAK
                     KLVPVGMELGGKDPLYVTEEVADLKQVAGAVAEGCFYNNGQSCCAVERVYINEKVYPQ
                     FMEYFSQEVKALKTGDPMDKAHNVGAVTRPVHIGFLESQVQEALDKGAQLVCGGKKIP
                     GPGSFFEPTILTNVNHTMRVMMEETFGPVIGVMKVKNDEEAIKLMNDCEYGLTAAVYS
                     KDIERAKNIVGQVNAGTSYINCCDRVSPYLPWAGRKNSGLGSTLSYLGILAFAKPRGW
                     HIRG"
     gene            complement(3813..4469)
                     /locus_tag="VIG33_13680"
     CDS             complement(3813..4469)
                     /locus_tag="VIG33_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEY8271935.1"
                     /translation="MSTVNPHYTFNYSQPKEYHFSHDSVFLSRRIFEHLAETDLTNCR
                     ALDICSGSGIVGMDFLFHRRGAGQSTPTSFDFLEIQEDYRPHFNENIQRLGPIETEFH
                     FVNRNYNDLQTPEFAEAYDLILSNPPYFFTWQGKLSPSEFKNRCRFFIDSDLKNLLLG
                     IAYSLKKTGVSYLLLRDLPEHGWSVMAEAEKICAGKLNVEVLGDVRGTHFVRLTPISP
                     "
     gene            4571..5233
                     /locus_tag="VIG33_13685"
     CDS             4571..5233
                     /locus_tag="VIG33_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271936.1"
                     /translation="MDNLIFFESKIKMPLMDLPVRSVLVPIGDHGVLISPGSRLKDSD
                     YKADLKVTTIVAPNLFHTAGVKKAQKYYPSSTLWGCEGADKKIPELAWGKIIGKDPWP
                     FQEILSLVTLHGMPSVNESVFIHKPSKSLIVTDLCFNMMEVSGFGARLILGLFGTYKK
                     LGTSRLFISGVKDKAAFTESLEELFSYDFEQIIVSHGSNITIDGKRKLHAALQERGFE
                     IK"
BASE COUNT         1535 a         1124 c         1195 g         1420 t
ORIGIN      
        1 aaaatgagcg ccaccatgga aagttatatg gcggggcttc tttattgtat gcctcatgtt
       61 ctacctatgt atgcccctac tattaattct tttaaacgat tggtggaagg agcctgggct
      121 ccgacgacag tcacgtgggg aatggacaat cgcaccactg ccgttcgtgc tctgccagga
      181 agttcaaaat ccacacgcat tgaattgcgg gttgtgggca gtgatgccaa tccctatctg
      241 gcgcatgctg ccgctctggc ttcgggtctc tatggtatca aacataaaat gaagctcagt
      301 gttccggcaa cgacaggtaa cggttatcgc gatctttcta acggaatcct gccagcgaat
      361 ctgtgggagt ccactcagac gatgaagaat tccaaagtcg ccgctgagat gtttggtgaa
      421 aattttgtaa aacattttac cggaactcgc gaatgggaat ggaggcaatt tgctaaaacc
      481 gttaccaact gggaatataa acgttatttt gaaatcattt aaataaacaa ggaagtgcta
      541 tgaatcacga ttggaataaa agatataatt ataattttcc tactcttgtt cgtttcggaa
      601 atggcgtcag cgaagaggtg ggaccccatt taaaagaaca gggcttgaaa aaagctttgg
      661 tggtttcaga tgccgcgcta attaagctcg cgttttttga aaattttatt tccactctta
      721 aaaaatcagg tcttgaagca gttgtgttct ctgacatcgc caaaaaccca gtgaagtcca
      781 atgtaatgaa aggggtcgaa gactatttga aaaacaactg tgattgtatt atcggcttcg
      841 gcggcggcgc cagcatggat gtcgccagag ctatcgctct aaaggcacac catactctcg
      901 atctgtttga ctatgatgat gccaaaggtg gcgatcgttt tgttaccgga accgtccctt
      961 atttcgtcac tcttcccacc acttcaggca caggctccga agtgggtcgc tccactgtta
     1021 tttctgacga catcactcat gaaaagaaaa ttttattttc acccaaactg atgcctcgta
     1081 aagtttttgc agatccaggt ttaaccatgg gcttacctgc atttgtcact gcaaccacag
     1141 gaatggatgc tttaactcat aatattgaag catacttatc caaaggcttc tcgcctcttt
     1201 gtgatggaat tgcattagaa ggtcttcgct taataggtga aagccttgaa cgcgccactc
     1261 acaacccgga ccttgaatcc agatctaaaa tgatgatggg agctctgatg ggagcaaccg
     1321 ccttccaaaa aggtttggga attgtgcatt cattggcaca tcctttatcg acagtcatgg
     1381 atatgcacca tggtttggcg aatgcaatta tgcttcctta tggaatggag tttaacaaat
     1441 ccgtttctgg tccacgcatg acaacgatgg cacgcacctt gggcttgaaa gatacaagtc
     1501 ctgatgggtt tattgcctgg attcgtgaac tcaataaaaa acttaagatc cctgcccgtc
     1561 tttcagagca aaacgtcaac agttcgcata ttgaaaaatt agctgagttg gccatcaatg
     1621 atccttgtca ttcctgcaac gaacgacccg tgactaaaaa agactttacc gccctctatc
     1681 aagaggcagt gcagtgataa aactgggagt cagcgcttgt tttatgcacc ccgatccagc
     1741 tcgaactgtc tttgggcaga aaacgcttac ctatttggaa cgcgacatga gccgctttct
     1801 ttcacggaaa gatgttatgc cgatcctgct cccggatgtc gcttgggagc agcttgagcc
     1861 ttatttaaaa gaaatggatg gctttgtttt tcagggtggc gctgacatca gtcctctcaa
     1921 ttacaatgag agtttgattg agaatggccg ctggcctggc gacccctatc gtgatcaata
     1981 tgaacttaaa atcatggact gggcttttaa aaataaaaaa cccatcttag ctgtttgccg
     2041 gggctttcaa ttagccaatg tttattttgg gggaagcctt taccaagatc ttaaagtgca
     2101 aacgaaaaca gaagtagaac accgcaacgc tgagctttat gacaaaattt atcatgaagt
     2161 cgaatgcacg cacggcagct tactagaaaa gatctacggc caaaaaaaat tatgggtcaa
     2221 cagcgtccat caccaaggtg ttaaagtttt agggaaaaac ttggtgattg atgctatttg
     2281 tcctaaagat caactgatcg aagcttttca ttaccagaat atggacgacc attttgtctt
     2341 ggctattcaa tggcatcctg aattttccca tacgattcaa gataaagtta taaaccctga
     2401 acccctctta aattattttt tggaaaggtt tgtatgaaaa ttgtaaatcc cgctaccaac
     2461 gctcttatta aggaactcag cgaagacaat aaggccacag tttttgaaaa gttcgctgtt
     2521 gcacaaaaag ctcagttaaa atggcaaaaa ctatccctcg atgaacgcct tgcttgtatt
     2581 aaaaaatttg aatcggcatt ggaccagaat aaagaaaatc tagctaagga tttaaccagt
     2641 gaagtgggaa aaccttatca agagtctttg aatgaaatca atggcgctag aaatcgcatc
     2701 agatttttca ttgaaaactc aaaaaaatgg ctctgtgaaa ataacgtaaa tcaagatggc
     2761 tccaccaaag aggttctcag ttttgatgct ttaggtgtga ttgccaatat ctccgcgtgg
     2821 aattatccat tcttggttgg aaccaatgtt tttattcctg cactcattgc tggaaacgcg
     2881 gtcctctata aaccttcaga gtatgcgact ctcacgggtc ttaatattga aaatcttttg
     2941 catgagtccg gcgtgcctgc gggcgtgttc acggcggtga ttggtgacgc caaagcaggg
     3001 gaagcccttc tcgatctacc tttggatggt tactttttca caggcagcta taaaaccggt
     3061 cgtgcgattg ctgaaaaagt ggcgaaaaag cttgtgcctg tcggaatgga attgggtgga
     3121 aaagatcctc tctacgttac tgaagaagtg gcggacttga aacaagtcgc cggagccgtg
     3181 gccgagggat gtttttacaa caacggccaa agctgctgtg cggtcgagcg cgtttacata
     3241 aatgaaaaag tttaccctca attcatggag tacttcagcc aagaagtgaa agctttaaaa
     3301 acgggcgatc caatggataa ggctcacaat gttggggccg tgacccgacc tgttcatatt
     3361 ggatttttag aatcgcaagt ccaagaggct ttggataaag gggcacaact ggtatgcggt
     3421 ggaaaaaaaa tcccaggccc tgggagcttc ttcgaaccta ctattttgac caatgtgaac
     3481 cacaccatga gagtgatgat ggaagaaact tttggacccg tcattggtgt catgaaagtt
     3541 aaaaatgatg aagaggctat taagctaatg aatgactgcg agtatggatt gactgccgcg
     3601 gtttactcta aagatatcga gcgggccaaa aacatcgttg gccaagtgaa tgcgggaact
     3661 tcttatatca attgctgtga tcgagtgagc ccttatttac cctgggcagg aagaaaaaac
     3721 tcgggtctgg gttcaacatt gtcttatttg ggtattttag cttttgcaaa gcctcggggt
     3781 tggcatatcc gaggctagtg ccgggtcttt ttttaaggac tgatcggagt caaacgcacg
     3841 aaatgggttc cgcgcacatc acccagaact tcgacattta acttacccgc acagatcttt
     3901 tcggcctccg ccatcacgct ccaaccatgc tccggcaaat cccgcaaaag caaataagac
     3961 actccggttt tcttaagact gtaggcaatg cctaacaaaa gattcttcaa atcagaatca
     4021 ataaagaaac ggcaacggtt cttaaattca gaaggcgaca gcttgccctg ccaggtgaaa
     4081 aagtaaggag gattcgacag aattaaatca taggcttcag caaactcagg ggtctgcaaa
     4141 tcattgtaat tgcgattcac aaaatgaaac tcagtttcga tagggcccag cctctgaata
     4201 ttctcattaa agtgaggacg ataatcttct tgaatctcca gaaaatcaaa agaggtggga
     4261 gtgctttgcc ccgctcctcg acgatgaaaa agaaaatcca ttcctacaat tcctgatcct
     4321 gagcaaatgt cgagagcgcg gcaattcgtc agatctgtct ccgcaagatg ctcaaagatc
     4381 cgccgcgaga gaaaaacgga atcatgggaa aaatgatatt ctttgggctg agaataattg
     4441 aatgtatagt ggggattgac tgtcgacatg gggcatgtcc tacctcactt cagggttttt
     4501 aacaatgcat aaggctctaa aggaaaagat tatcttgaga ttctccactc caaatgaata
     4561 tgattcctct atggacaacc tcatcttttt tgaatctaaa atcaaaatgc cactgatgga
     4621 ccttccagtt cgaagcgtgc ttgttcccat aggagaccac ggagttctca tttcacccgg
     4681 atccagatta aaagacagtg attacaaggc agatctgaag gtgactacga tcgtggcccc
     4741 aaacctattc catacagcag gagtgaagaa ggctcagaaa tattatccct ccagcacgct
     4801 ttggggatgc gaaggagcag ataaaaaaat tcccgagctg gcctggggca agattattgg
     4861 aaaagatcct tggccatttc aggaaatttt gagtttagtg acccttcatg gaatgccttc
     4921 tgtcaatgaa agcgtcttta ttcacaagcc ttccaaatcc ctcattgtca ctgatttgtg
     4981 tttcaatatg atggaggtga gcggcttcgg agcacgcttg atcctagggc tctttggtac
     5041 ctataaaaaa ctgggcacta gccgcctgtt tatctcaggg gttaaggata aagcggcctt
     5101 cacagagtct ttagaggaac tcttcagcta cgactttgag caaattattg tcagccatgg
     5161 cagcaatata actattgatg gcaaacgtaa acttcatgcg gctctgcaag agcggggctt
     5221 tgaaataaaa tgattcttaa tatgtaaatc agtgcaaagg cttgtaacct tcat
//
LOCUS       DATBTE010000366        17812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1756245, whole genome shotgun sequence.
ACCESSION   DATBTE010000366 DATBTE010000000
VERSION     DATBTE010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 17812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17812
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756245"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..637
                     /locus_tag="VIG33_13690"
     CDS             158..637
                     /locus_tag="VIG33_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271937.1"
                     /translation="MPDVDANHDGFISKSEAQSWPEFQWPWNPQQDRISFSEIEIALH
                     ANSDLQKIYQQASAAKIWQGVFNRKPIYDEASKLSQNIYVFTGELDVQTRPDEALKLQ
                     KACVLNQKLNCKIHLVPGLGHGMSKPKGPRRQKLIDATLGPVDDFFVKLLERISERL"
     gene            981..2282
                     /locus_tag="VIG33_13695"
     CDS             981..2282
                     /locus_tag="VIG33_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271938.1"
                     /translation="MDLNTSVLIVSAYGRGYWLAAELQRENIPVVLIDVTSQLGVWPP
                     EDLEGPFGFFRPDEMTDSQGERLFCEDPFESVDHGFTVWIEDGPLELKSSLTEYRLQK
                     NGQNKEVYESLMSGKSGPKNNFKSNSGIRKWASENFKKSWVMHLAHQWAATTYVPSAQ
                     ASLAGKPLKLMNPFSVRKATRAGQQKSFEWLSQKKVEVYQKTEILDLSFETKKTISGV
                     ETHGERSGLLHLDQVVWMLNSEESYFVTPKIAKSLFPEGPLEPEWCWIRYRLKMSKCP
                     ERDSLPIHLLLLSQVTGPWTHQNLMVIQRTALEDQFDVWIRIPNVQRFNKEYLRIRGE
                     KVMEILRERLPLALPEIQSYPQEFYYTYSQIGPSSFPVYGEGVESRRSHTKFSNVYLD
                     GAEIWKNYSWDDQFENQGLIRDSLLKWWKLLQQRKEKERRD"
     gene            2275..3585
                     /gene="purB"
                     /locus_tag="VIG33_13700"
     CDS             2275..3585
                     /gene="purB"
                     /locus_tag="VIG33_13700"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY8271939.1"
                     /translation="MIERYTRPEMGIIWDLDHRFGKMLEVEIALAQVQAQMGLIPQAA
                     AKSIAQKGRFNAKRIGEIEKQTKHDVIAFVSNVAENVGPHGKFIHFGLTSSDVLDTAF
                     SLQVREAGVLLSKSMDSLERTLLSLVRKHEDTLCAGRTHGMFAEPTTFGFKMLGYLSE
                     FRRNRERVLRALEQMMICKLSGAVGTYSSMGMEVENKVAKKLRLKPETIATQVIPRDR
                     HAEMILSLAFMMGGLERLAVELRHLQRSEVGEVTEGFSKGQKGSSAMPHKKNPISAEN
                     ITGLARLVKGYAFTAMENIPLWHERDISHSSAERVIFPDAFIVTDYAVSRMATVLEGL
                     EVNKKRMLENIESSQGQLFSSHVLLALVDKGLSRETAYAHVQRLSHSLSKGEHLREKI
                     LQDSEVAKYLKAKELDEIFNGKKHKKTIKAAMKARLKTAMKRRT"
     gene            3585..4505
                     /locus_tag="VIG33_13705"
     CDS             3585..4505
                     /locus_tag="VIG33_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEY8271940.1"
                     /translation="MSWNYLQKGDVVDVVSPGFASTPQEVESARRFLLKWGLQPRIPH
                     KLIQKHFLHSNSDEKRFGFLREALEAHDSKIIWCLRGGYGSNRLLPHLDKIKKPRRQK
                     LLVGISDITSLHIYFEQKWNWPTLHASLLDRLGSGKVPAKIEKETMDLLFGKNKSVEF
                     KKLKAMNKAALNLKKVKSSVVGGNLVVLQSTLGTPYQVKTEGKILFIEDLGERGYRVD
                     RILEHFHQVGVFKKCKGLVIGEFLGGQEPDGKVLWPQVFKSWAERLEIPVFIGMEAGH
                     GVVQRPVPLYTEAILAGGKKGSLWINAEGR"
     gene            4502..5563
                     /locus_tag="VIG33_13710"
     CDS             4502..5563
                     /locus_tag="VIG33_13710"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY8271941.1"
                     /translation="MKYSVLEKKLMEQLKSRITDTTPGVVVRAYQGGRMVCDVSVGDT
                     FAYYDLASLTKVIFTVQALMVAFEQGKWDLDTHVQQYLPWFPHGGVRVQDLLTHSSGL
                     AWWMPFYKEMDFSLPLEKRREKLKEILSKLEVSDTPNSVYSDVGFLVLGFLVEEFYKD
                     NLLSVWGGIKDKFYAGTTLEFHRDNVAKHRLSLYSPTEECPWRKKLIVGEVHDENCWA
                     LGGVSTHAGLFGSMDDVSWALLNIRSQLLGIARYSVKQKTAKLFASRARPQGMGDWAL
                     GYMMPTPGAASCGNHFSLMSIGHTGFTGTSMWYDPKADFGVVILSNRVLYGRENKSFA
                     KLRPEIHNWIVEGFRKSAV"
     gene            5560..7008
                     /gene="mpl"
                     /locus_tag="VIG33_13715"
     CDS             5560..7008
                     /gene="mpl"
                     /locus_tag="VIG33_13715"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEY8271942.1"
                     /translation="MNSSVHLKTNSHIHLMGICGTAMASLAGLLKERGFKVTGSDLNP
                     YPPMSTQLDSLGIPIMKGYKAENLHPNPDFVIVGNVISANNEEAQELLRLKLPHTSLP
                     KAMGEFIIADKECINISGTHGKTTTTSLMSWVAEICGEKPGFLIGGIPKNFAKSFRNP
                     EKKFFVIEGDEYDTAFFDKVPKFIHYKPRHVILTSVEFDHADIYKDLDAVKAAFRTLI
                     KLVPEDGTLMACADDANVMELFKESKCKNSFTYGLKAGDFKTTILRETTDGIEFQVEF
                     SSKPKFKSQSKNEVLGPFFVKMSGEYNILNATSVVAMSQVLGFSPEKVKEALRSFEGV
                     KRRQEILGEPKGILVIEDFAHHPTAVRETVKGIQNKYPGRKVFSVFEPRSATSRRKVF
                     QQDYVKAFKGSHEVMLAEAFDQSKIDAENRFSSMELIHDLKKSGVTAEVFSSTEAIVN
                     ALKSKAQCGDIVLIMSNGGFDGIYTKLLSALS"
     gene            7120..9708
                     /gene="mutS"
                     /locus_tag="VIG33_13720"
     CDS             7120..9708
                     /gene="mutS"
                     /locus_tag="VIG33_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY8271943.1"
                     /translation="MIPTTLSTTLSTPTNSENLTPLMKQYWDIKSLHQDKILLFRMGD
                     FFEMFYDDAVKAAPILGIALTQRNKKSQDETPMCGMPHHSISGPINKLLAHGFKVAIC
                     DQVEDPKTAKGIVKRAVTRVLTPGMVYDSDTLDGTMAHYLVSFDKDSMSFLDTTTGES
                     FFFRTKDFATFKRFLEVLPVAEIVLSSEEKSFFEEEQKKTSGLQWILVSLHEETAEPL
                     HPLLVQGAPLSAARLLSYVKSLGGEESLKTLSPFEERDFQQRLELTPITLRHLEIFST
                     YKGEGLGSLFFAINRTNTSAGSRLLRQWMSFPLVNPQSIETRYGQVDLWRNHLAELKR
                     VRQILSQMGDIERRLGKISQPQCNGRDLLALAQSIRAGLSALEVSLILRPLENVKVAA
                     IEKLQQKLQTLFLKIENTLVEEPPLATKQGYLIRPGVSPELDELFELSTQSQSLLAKM
                     EMEEREKTGIASLKIRYNNVFGYYIEITHTHKDKVPKHYQRKQTLSNAERYCTEALIE
                     LERKVLTAQAKRGDLEFEYFENLRREVLSAGSELMILAHECSELDVISSFAWLSLEEK
                     YIRPQFSEKRDLLLKSSRHPVVEQTVKKNFVPNDIELKAGACLLLTGPNMAGKSTLMR
                     QVALTAILAQIGCFVPADQAVLPIFDAIFTRIGASDQLSEGLSTFMVEMTETAAMLKN
                     STQKSLVILDEVGRGTSTFDGMCLAQSILEHLLSDVKTTTFFATHYHELTQLEDSYPQ
                     IQNAHMTVADTQGEIRFLHTLVRGPAKKSYGIQVAELAGLPPVVIRRAKSILKSIESQ
                     KVGATSQLSLLDMAFEEETSVSPEEKAEQERLWTFLQEVKKYPLTKITPLEALNQIAQ
                     WQKSLL"
     gene            9705..12059
                     /locus_tag="VIG33_13725"
     CDS             9705..12059
                     /locus_tag="VIG33_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY8271944.1"
                     /translation="MSRTYLSDEQWEKAKEVFPPVFWTPNGDIRFTSYFGGFQFAGWL
                     SSQLEEKLKSFPGFLECEPVSLGSWSRGELCPLSDIDILFCGAEAQVKTLTDRLHQAG
                     LRVRYRMPKNPEDWTEGVESFDVLALWKAKPLSSASASKLLLQQKKLWENKNELRAKL
                     LKDLLQEREQRAERYNSISNYLEPNLKYGPGGLRDIEQALQVYDLFSEKFKNPAHALS
                     VLNYHKAYILTLRQKLHLMECGEVLVSSVQSELGQFFNFKNHKDFMRDLERGLSRSHF
                     YVNWIFSIAESPQTLLAKTRGLKFNKPEDLISALRQYPNPLGQQRVRENLDRLLPEKV
                     SAKSSRWFGKVLQEILSPKVSKDLLISAFESRLIDKILPAIRHLVGYVQHDQYHRYTA
                     DVHIMQACVMLKVFYKNPKALGPLSAFHRELNEEDWKILSWAALYHDLAKGLHSESHA
                     DLGVEILRKDFKKYGFAKKITEEVAWIVKYHLELSIAAFRKNPKAKKTWEDLEAIGAK
                     GARLKRLAVFTVMDICATNPEAWNEWKGLLLRDLVEALNAPSTKNYFSLKTSLQKKKL
                     KGLEAVLEDFDDFLLESLSASVLAEDLKAARESSESLSPLAFTKGKEIWVRFHSKEDQ
                     EGLLAEYVQRLYSLGLGIRHAAIQTLNGVGVYDWFQVSTSKNPQVIEKILKNSQGLIK
                     DRAKESEKNIPKTLPKVIYDQIEWVSIDDNEWVLSFRGRDQAGFLTLATKTLAAMRLN
                     IKSARVHTWGRQVNDIFILKKAPQTPEILLAEIKKRLGESSLFA"
     gene            complement(12086..12286)
                     /locus_tag="VIG33_13730"
     CDS             complement(12086..12286)
                     /locus_tag="VIG33_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271945.1"
                     /translation="MGIGAKVLDFLFGKDPDIFDEKGNVSHKLPKKKWDAWYNRTKTD
                     PHHNWRNHTGITGSGSKKKTQN"
     gene            complement(12389..12655)
                     /locus_tag="VIG33_13735"
     CDS             complement(12389..12655)
                     /locus_tag="VIG33_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271946.1"
                     /translation="MKKALVMTLVLAISSISFAWIEKAPQAKSYEFKFKLKAESFVFS
                     SSALSYDEAFEVAAKACFKHYKRGRHVSEDQGLDIIDVCANPRS"
     gene            12865..13698
                     /locus_tag="VIG33_13740"
     CDS             12865..13698
                     /locus_tag="VIG33_13740"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HEY8271947.1"
                     /translation="MNVFGLFLAFVLSFSVQVMARERTLVIVQVEDTLKLSHVQNFWD
                     SLNYNTDTKQRFIGMNAALSELARKNAASTFAYLTQGPEMAAGEAEMEFISVNGFPRG
                     QFVPYSNNADRDTQMKVLRKLIKTSNPARVILMSHNGGLDPEIFHGIAMEFKDIYFLQ
                     YLHVIYSITSPTEMGSSLFLEQTGFVTAVELLVDWQLKGLVGLKETITLAQLLIPKIV
                     YENLDSPGIVEYGVPRFVNCDDFQWKWVAKNDYSFLVSLRDHLIERCHMQISPLGSPA
                     H"
     gene            13698..14513
                     /locus_tag="VIG33_13745"
     CDS             13698..14513
                     /locus_tag="VIG33_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271948.1"
                     /translation="MKLKYQLPRIYEDLLPRGLLQIEPLETKATCDQCIMARPQNKGK
                     IFYTPDLKCCTFYPFLPNYIVGAILTYETSSAPSAVAVIRSKIAKREYSLPLGLVAPP
                     NYQVPYNQRRQNEFGQREDWLCPYFNKEQQNCGIWRQRGAVCTSFYCKSSYGVKGLKF
                     WDELSNYLTYVEMALMEESLVMLDFSPRQISDLLNYLNRQEATKTEMKSSRMPEERSR
                     RLWNGYYEDQEAFFKKSFEIVKKLDKNSFRELLGEPGKQLEERLQKAFEKICP"
     gene            14504..14947
                     /locus_tag="VIG33_13750"
     CDS             14504..14947
                     /locus_tag="VIG33_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEY8271949.1"
                     /translation="MSMIEERQKQIISEFENLKDWEERYKKIIEMGRQLTEMPENLKT
                     EENKVKGCQSQVWLFAQLTPQGEMILQGDSDAMIVKGLVALLLRVYSGANPNEILKTP
                     PEFLKTLGFEGHLSPSRSNGLHAMLKQIRFYATAFAYVLNSSKKS"
     gene            15027..15890
                     /locus_tag="VIG33_13755"
     CDS             15027..15890
                     /locus_tag="VIG33_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY8271950.1"
                     /translation="MVKSVLLVAIGYFLSPIAIAKIQDRVIYGDDNRVEFFQVTRADV
                     REVADSTAALIRNSNLEAKGDGNTTVKAETFQKFAGLCSTERFAAEPSGAFCSAFLVA
                     DDMVATAGHCIDASRCPQVSFVFSYRMSEQSQAPVEVNSSEVYRCTQVLEREQTGAQD
                     YALVRLDRSVRGHRVLTLAKSSVNVGDNLITVGHPSGIPTKVTDKGLVRSVKAEFFTA
                     NLDTYGGNSGSAVFNQDTLEVAGILVRGENDFIYDSVNKCSVSNRCSAEGCRGEDVTQ
                     ISYISEALKKY"
     gene            complement(15956..16693)
                     /locus_tag="VIG33_13760"
     CDS             complement(15956..16693)
                     /locus_tag="VIG33_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY8271951.1"
                     /translation="MPNISEFDFMAAMGKSWKNAGKVEKAMQKGQIFTKLAREISVAA
                     KAGGPDPAGNSRLRLAIDDAKKMSCPNDTIDRAIKKGAGLLDDGKIIEEVVYEGYGPH
                     GVGVIVECQTDNKNRTASEMRHAFKSHDGNMGETGSVAWMFEHVGYLIGTKAGTFDPD
                     EEAIEAGANEVYKDDDGAYEFYTSHNDLIKVNEALTKRGWKITTSELSYKAKTPTVLD
                     EAQLKEVEEFLNFLDDLDDSHKVHVTL"
     gene            16721..17497
                     /locus_tag="VIG33_13765"
     CDS             16721..17497
                     /locus_tag="VIG33_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF455 family protein"
                     /protein_id="HEY8271952.1"
                     /translation="MFSLEVPDVWKKIKNIQKSCESLLEGDSFILPPYPARDVEVLDS
                     KLHPPKVGFSKIEGQARMLHDLASIELQAMEIGLRTLAEFPEAPPDFREELVEVTVSE
                     ANHLRLCLEGIQDLGFKWGDWPVHCALWDAVSAEDSLLDRILIVHRYLEGSGLDAGSY
                     LMQRLDGVRDGKLARQIVGQINFEEIGHVGFGSQWYRKICQLEKIDATEDFPRRMEAL
                     RMKLPKRMEKINRELRRQAGFTEQEIDYCEVYRKSILPQK"
     gene            complement(17618..>17812)
                     /locus_tag="VIG33_13770"
     CDS             complement(17618..>17812)
                     /locus_tag="VIG33_13770"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY8271953.1"
                     /translation="FEKAQHCCEYTHPLTGKRCGSEFQLQIDHVRPLACGGSDEIKNL
                     RALCGLCNRREAQRWGLKSL"
BASE COUNT         4831 a         3603 c         4357 g         5021 t
ORIGIN      
        1 agttgatttt cgatcatgac gcacgcttca tattccaata tttcctgcac ttcttctcgt
       61 attaattaag agtttaattc tcattggatt tgccggggag aatatggcga caactttgga
      121 ttggcagtta tttcgaagac ctatcgactc ttgactcatg ccagacgtcg atgccaatca
      181 tgatggcttt atttcaaaat ccgaagcaca gtcatggcct gagtttcagt ggccgtggaa
      241 tcctcaacag gacaggattt cattttctga gatcgagatt gctcttcacg ccaattctga
      301 tcttcaaaaa atatatcagc aggcttcagc cgcaaagatt tggcagggag tttttaatcg
      361 aaagccaatt tatgatgagg cttccaagct gagtcaaaat atctatgtat tcaccggaga
      421 acttgatgtg caaactcgcc ccgatgaagc gttaaaatta cagaaagcct gcgttttgaa
      481 tcaaaagtta aattgtaaga ttcatcttgt tccggggctg gggcatggaa tgtcgaaacc
      541 aaaaggccct cgaaggcaga aactcattga tgcgacattg gggcccgttg atgatttctt
      601 cgtgaaactt ctggaaagga tttctgaacg cttatgattg aaatttggca cgtatccgaa
      661 gcatcttgtt taagagcttc agtaacaact gttttcatcc ggatgataaa tagtgataag
      721 gattattcaa tatgttccac aatagttcca caacggttcc acagtgttcc acaaccgctt
      781 tcatacaaaa atagcattga gcccatgagg tcgcagaatt gctgaggggc ctgctcttgc
      841 ggtgagagct tgcgtcatta tgagtttctg atttcattgt gactattaaa aattataaaa
      901 taaacgacca tgatgtgaga ggggctcaaa aacccaactg agtctttgac tttgaagatc
      961 aggaattttc taatcatttt atggatctaa atacttctgt acttatcgta tcagcctacg
     1021 gccggggata ctggctggcg gcggagcttc agcgggaaaa cattccggtt gtgctcattg
     1081 atgtgacatc tcagctaggg gtttggccac ctgaggatct tgaaggcccc tttggctttt
     1141 tcagacctga tgaaatgaca gactctcagg gggagcgtct tttctgtgaa gatccttttg
     1201 aatccgtcga tcatggtttt accgtttgga tcgaagatgg acccttggag ttaaaaagct
     1261 ccttgacgga ataccgtctt caaaaaaatg gtcagaacaa agaagtctat gaatcattga
     1321 tgagtggaaa gtcaggccct aagaataatt ttaagagcaa ttctggaatt cgaaagtggg
     1381 cttcggaaaa ttttaaaaag tcttgggtga tgcacttggc ccaccaatgg gctgccacca
     1441 cctatgttcc gagtgctcaa gctagcctgg cggggaaacc tctaaagcta atgaatccgt
     1501 tttcagttcg caaggccact cgagccggtc agcagaaatc ttttgaatgg ttaagtcaga
     1561 aaaaagtgga agtttatcaa aagactgaaa ttctcgatct ttcttttgaa accaagaaaa
     1621 ccatttccgg cgtagaaacc catggtgaac gttccggact cctgcacttg gaccaagtgg
     1681 tgtggatgct taatagcgaa gagtcttatt tcgtaacccc aaaaatcgcg aaaagcctat
     1741 ttcctgaagg gccgctggag cccgagtggt gttggattcg ctatcgtttg aagatgtcga
     1801 agtgccctga acgagacagt cttcccattc atttgttgct cttaagtcaa gtaactggac
     1861 cctggactca tcaaaatttg atggttatac aaaggaccgc tcttgaagat cagtttgatg
     1921 tttggattcg aattccgaat gtgcaaagat ttaacaaaga gtatctgcgt attcgcggcg
     1981 aaaaggtcat ggaaattttg cgcgagcgtt tgcctttggc tttgccagag attcaatctt
     2041 accctcagga gttttattat acctattccc aaattggccc ttcttctttt ccggtttatg
     2101 gagaaggcgt tgagtctcgc cggagccata cgaagtttag caatgtttat ttggatgggg
     2161 cggagatttg gaaaaattat tcttgggatg atcaatttga aaaccaaggt ttgattcgtg
     2221 attctttatt gaagtggtgg aaactcttac agcagcggaa ggaaaaagag cgtcgtgatt
     2281 gaacgttata ctcgtcccga aatgggcatt atttgggatt tagaccatcg ttttggaaaa
     2341 atgcttgaag ttgaaattgc cctggctcag gttcaagccc agatggggtt aataccccag
     2401 gccgcggcaa agtcgattgc acaaaaaggt cgctttaacg caaagagaat tggcgaaatt
     2461 gaaaagcaaa cgaaacacga tgtgatcgct tttgtctcga acgtagcgga aaatgtcggt
     2521 cctcatggaa agtttattca tttcggtctc accagttcgg atgtcttgga tacggccttt
     2581 agtttgcaag ttcgtgaagc cggagttctg ctgagtaaat ccatggacag cttggaaaga
     2641 acattgctgt ctttagtgag aaaacacgaa gacacccttt gtgctggacg cacccatgga
     2701 atgttcgcgg agccaacaac ctttggtttt aaaatgctgg gatatttgtc ggagttccgt
     2761 cgcaatagag aacgggtttt gagagccctc gaacaaatga tgatctgcaa gttgagcgga
     2821 gccgtcggca cctattccag tatgggaatg gaagttgaga ataaggttgc caagaagctg
     2881 agattaaaac ctgaaactat cgccacccaa gtcatcccca gggaccgtca tgcggagatg
     2941 attctcagtc tcgcctttat gatgggaggc ctggaaagac tggctgtgga gcttcgtcat
     3001 ctgcaaagaa gtgaagtggg agaagtgacg gaaggcttta gtaaggggca aaaaggttct
     3061 tcggccatgc ctcacaaaaa aaaccccatc agtgcggaga acatcacagg gttggctcgc
     3121 ttggtaaaag gttatgcttt taccgccatg gagaacattc ccttgtggca tgaacgggat
     3181 atcagtcact cctcagcgga gcgggtgatc ttccctgatg cctttattgt cacggactat
     3241 gcagtgagca gaatggccac ggtcttggag ggtttagaag tcaataaaaa gcgtatgttg
     3301 gaaaatattg aaagctctca aggtcagctc tttagttctc atgttttgct ggctttagtt
     3361 gataaagggc tcagtcgtga gaccgcctat gctcatgtgc aaaggctcag tcatagtctg
     3421 tctaaggggg aacatctgcg cgaaaagatt ttgcaggatt ctgaggttgc aaaatattta
     3481 aaagctaaag agctggatga aattttcaat ggcaaaaaac ataagaaaac cattaaagcc
     3541 gccatgaaag cgcgcctaaa aaccgcgatg aaaaggcgga cttagtgagc tggaattatt
     3601 tgcaaaaagg tgatgtcgtt gatgtggttt ctccaggttt tgcgtccacc ccgcaagagg
     3661 tggaaagcgc ccggcggttt ttattaaaat ggggattgca gccgcgcatt ccccataaat
     3721 taattcaaaa acattttttg cattccaata gcgatgagaa acgttttggc tttttacggg
     3781 aagctttaga ggcccatgat tcaaaaatta tctggtgtct tcgcgggggg tatggctcaa
     3841 atcgactttt gcctcatttg gataagataa aaaaacctcg gcggcaaaaa ctgcttgtgg
     3901 gcatcagtga tatcacttcg ttgcatatct attttgaaca aaagtggaat tggccgacgc
     3961 tgcatgcgtc tcttttggat cgcctgggaa gtggcaaggt tccggcaaaa atagaaaaag
     4021 aaaccatgga tcttttgttc ggaaaaaata agagtgtgga gtttaaaaaa ctcaaagcga
     4081 tgaataaagc ggctttgaac ttgaaaaaag tgaaatcctc cgtggtgggc ggaaatctcg
     4141 tggttttgca aagcactctg gggactcctt atcaagtaaa gacagagggt aaaattcttt
     4201 ttatcgaaga tctcggggaa cggggctatc gagtggatcg cattttagaa cattttcatc
     4261 aagtgggagt gtttaagaaa tgcaaaggtc ttgtgatcgg tgagttttta ggcgggcagg
     4321 agccggatgg aaaagtttta tggcctcagg tttttaaatc gtgggcagag cgcttagaaa
     4381 ttccagtctt cattggaatg gaggccggcc atggcgtggt gcaaagacct gtgccgcttt
     4441 atactgaggc aatcctggcg ggcggtaaaa aaggctctct atggatcaat gcggagggga
     4501 gatgaagtac tcagtccttg aaaagaaatt aatggagcaa ttgaaatccc gcatcaccga
     4561 caccacccct ggggttgtgg tccgtgctta tcaaggcggg cgcatggtat gtgatgtgag
     4621 cgtgggggat acttttgctt actatgacct cgcgagttta accaaggtga ttttcacggt
     4681 tcaagcattg atggtggctt ttgaacaagg aaagtgggat ctcgatactc atgtgcaaca
     4741 atacttgcct tggtttccgc atgggggtgt gcgtgtgcaa gatcttctga ctcactcttc
     4801 aggtcttgct tggtggatgc ctttttataa agaaatggat ttttctttgc ccctggaaaa
     4861 aaggcgagag aaattaaaag agatcctctc taagttggaa gtgtctgata cccctaacag
     4921 tgtttattcg gacgtgggct ttttggtttt aggcttcttg gtggaagagt tttacaaaga
     4981 taatttatta tccgtctggg gcggaattaa agataaattt tatgcgggaa cgactttaga
     5041 atttcaccgg gacaatgtgg cgaaacatcg cttgagtctt tattctccaa ccgaggagtg
     5101 cccgtggcgt aaaaagctga ttgtcggtga agttcatgat gaaaactgct gggcattggg
     5161 cggcgtttcc actcatgcgg ggctttttgg cagcatggat gatgtgtctt gggctttact
     5221 gaatattcgc tctcaacttt tgggtattgc gagatattca gtaaaacaaa aaaccgcgaa
     5281 attatttgcc tcccgggcac gtccgcaagg gatgggcgat tgggctttgg gttatatgat
     5341 gcctacccct ggcgcggcca gctgtggaaa tcatttctct ttgatgtcta ttgggcatac
     5401 gggttttacc ggaacttcga tgtggtatga tccgaaagct gactttggcg tggtgatttt
     5461 atctaacaga gtcttatacg gacgcgagaa taaatctttt gcgaagcttc gtcctgaaat
     5521 tcataattgg atcgttgaag gattcagaaa gagtgctgtg tgaactcgag tgttcatttg
     5581 aaaaccaata gtcatattca tctcatggga atttgtggaa ccgccatggc aagccttgca
     5641 ggcttgttga aagagcgggg atttaaggtc accggaagtg atttgaatcc ctatcctccg
     5701 atgtccacgc agctggactc cttgggcatt cccattatga aaggctataa ggctgaaaat
     5761 ctccacccca atccggactt tgtgatcgtt ggcaatgtca tttcggccaa taacgaagag
     5821 gcgcaggagt tgttaaggct gaaactgcct cacacctcat tacccaaggc catgggtgag
     5881 tttattatcg ctgataaaga gtgtattaat atttctggga cccacggaaa aaccacgacc
     5941 acctctttaa tgagctgggt ggcggagatt tgtggggaaa agccgggatt tctcatcggt
     6001 ggaattccta aaaattttgc gaaatctttc agaaatcctg aaaagaaatt ttttgtgatc
     6061 gaaggggatg agtacgacac ggcttttttt gataaagtgc ccaagtttat tcactataaa
     6121 cctcgtcacg tgatcttgac ctcggtggag tttgatcatg ctgatatcta caaagatctg
     6181 gatgcggtga aggccgcctt tcgtactttg attaaactgg tgccggaaga cgggacgctg
     6241 atggcttgtg cggatgacgc caatgttatg gaacttttta aagaatcaaa atgcaagaac
     6301 tccttcacct atggtcttaa agcgggagat tttaaaacaa ccattcttcg agaaaccact
     6361 gatgggattg agtttcaggt cgagtttagc tctaagccta agtttaagtc tcagtctaaa
     6421 aatgaagtgc tcggtccttt tttcgtaaag atgagcgggg aatacaatat tttaaatgcc
     6481 acttccgtcg tggctatgtc tcaagtttta ggcttttctc ctgaaaaagt gaaagaggct
     6541 ctgcgaagtt ttgagggagt gaaacgacgc caagaaattc tcggagagcc gaagggcatt
     6601 ctggtgattg aagattttgc gcaccaccca acggcggtac gagaaacggt caaaggcatt
     6661 cagaataaat atcccggccg taaagtcttc tcggtttttg aacctcgctc cgcaacttca
     6721 cggcgaaagg tcttccaaca ggattacgta aaggctttca agggctccca tgaagtgatg
     6781 ttggcggagg cttttgatca atctaagatt gatgcggaaa atcgtttttc gagcatggaa
     6841 ttaatccatg atttgaaaaa atctggagtc accgcagagg tgtttagctc gaccgaggcc
     6901 atcgtgaatg cgttgaagtc caaggcccag tgtggagata ttgtcttaat catgagcaat
     6961 ggggggtttg acgggatcta caccaagctt ctttcggcgc tgtcctaggc tcgatcctaa
     7021 gctttgtcct gaaacgcacc gcatctttct tgctagtttt tttggctcta gttaacactg
     7081 agccaataac ttccacaatg atatcgaatg gcgccgacta tgataccaac cacactttct
     7141 acaaccttat caactccaac aaatagcgag aatttaaccc cgttaatgaa gcaatattgg
     7201 gacattaaat ccctgcatca agataaaatt ttgcttttcc gaatgggtga cttctttgaa
     7261 atgttttatg atgacgccgt gaaggcggcg ccgattttgg ggatcgccct gacgcaaaga
     7321 aataaaaaat cccaggacga aactccgatg tgcgggatgc ctcatcattc tatctctggg
     7381 cccatcaata aacttctcgc ccatggtttt aaagtcgcta tttgtgatca agttgaggat
     7441 cctaaaacag ctaagggcat tgtgaagcgg gcggtgactc gagtcctgac ccccggcatg
     7501 gtttatgatt cggatacttt agatggaacg atggcccact atttagtgag ttttgataaa
     7561 gactccatga gttttttgga cacgaccaca ggagagtcct tttttttcag aacaaaagac
     7621 tttgcgacct ttaaaagatt tctagaggtt ctgccggtgg cggaaatcgt cctttcttct
     7681 gaagaaaagt ctttctttga agaagaacag aaaaagacct caggtctgca gtggattctc
     7741 gtcagtcttc atgaggagac cgctgaacct cttcatccgc ttttggttca aggggcaccg
     7801 ctatcagcgg cgcgcttgct ttcctatgtt aagtccttgg gtggcgaaga gtccttaaag
     7861 actctgtctc cctttgaaga gcgggatttt caacaacgcc ttgagcttac gccgatcact
     7921 ttaaggcatc tcgaaatctt ttcgacctat aagggggagg ggctcggctc tttgttcttt
     7981 gccatcaatc ggacaaatac ttcggcgggc agtcgtctgc ttcgtcagtg gatgagtttt
     8041 cctttagtaa acccgcagag tattgaaact cgctatgggc aagtggatct atggagaaat
     8101 cacctggctg aattgaagcg cgtgcgccag attctttcgc agatgggaga tatcgagcgt
     8161 cgtctcggaa aaatttctca gccccagtgc aatggtcgtg atctgttggc cttggcgcag
     8221 agtatccgcg ctggtttaag cgccttggaa gtgtccttga ttttaagacc tctggaaaat
     8281 gtcaaagtcg cggcgattga aaaactccaa caaaaactgc aaactctttt tttaaaaatt
     8341 gaaaacactt tggtggaaga acctcctttg gccactaagc aaggttacct gatccgtccc
     8401 ggagtttctc cggaattgga tgaattgttt gaactttcga ctcagtccca gagtcttctt
     8461 gcaaaaatgg aaatggaaga gcgggaaaaa accgggattg ccagccttaa aatccgttat
     8521 aacaatgttt ttgggtatta cattgaaatc acccataccc ataaagacaa agtgccgaag
     8581 cattatcagc gcaagcaaac cctttcgaac gcggagagat actgcaccga ggctttgatc
     8641 gaactggaac gaaaggttct cacggcgcag gcgaaacgcg gcgacttgga gtttgaatat
     8701 ttcgagaatc tgcgtcgtga agttttgtca gccgggtctg aattaatgat cctggcccat
     8761 gaatgcagcg agctggatgt gatcagttct tttgcctggt tgagtcttga agaaaaatac
     8821 attcgacccc agttttctga aaagagagat ttactcctta aaagctcccg tcatcccgtt
     8881 gtggaacaga cggtgaaaaa gaattttgtt cctaatgata ttgaactcaa agctggggct
     8941 tgtttgttat taaccggtcc gaatatggcc ggtaaatcaa ccctcatgcg gcaagtggct
     9001 ttgaccgcga tcttggcgca aataggatgt tttgtaccgg cggaccaagc ggtcttgccg
     9061 atttttgatg cgatctttac gcgtatcggg gccagtgatc agctttcgga aggcctttcg
     9121 accttcatgg tggagatgac cgaaacggcg gccatgctta aaaactccac gcaaaagtct
     9181 ttagtgattt tggatgaagt cggccgcggc accagtacgt ttgatggcat gtgcttggct
     9241 caatccattc ttgagcatct gttatcggat gtgaaaacca cgacgttctt tgcgacccat
     9301 taccacgaat tgactcagtt ggaagattcc tatccgcaaa ttcaaaatgc ccatatgacg
     9361 gtggcggata ctcagggtga gattcgtttt ttacatactt tagtacgggg gccggcgaaa
     9421 aaatcctatg gcatccaagt ggcggaactg gcgggtttac ctcccgtggt cattcgccga
     9481 gccaaaagta tattgaaatc cattgagtcc cagaaggtgg gggccaccag tcagttgtcc
     9541 ctcttggata tggctttcga ggaggagacc tcagtcagtc cagaagagaa ggctgaacag
     9601 gaaaggcttt ggactttttt gcaagaagtg aaaaaatatc ctctcactaa gatcactcct
     9661 ctggaggctt taaaccaaat tgcccaatgg caaaaaagtc ttttatgagt cgaacctact
     9721 taagtgatga acaatgggaa aaggccaaag aagtttttcc tccggtgttt tggactccga
     9781 atggcgatat tcgttttact tcgtattttg gtggattcca gtttgcgggc tggctcagtt
     9841 ctcaactaga agaaaagctt aaatcttttc ctggattttt agaatgtgaa ccggtgagtc
     9901 tgggatcttg gtcccggggt gagctctgtc ctctctctga tattgatatt ttattttgtg
     9961 gtgcggaagc ccaagtaaaa actctgacgg atcgtttgca ccaagccggg ttaagggtgc
    10021 gctatcgcat gccgaaaaat cctgaagact ggactgaagg ggtcgagagc tttgatgttt
    10081 tagccctgtg gaaagccaaa ccattaagtt ccgcctccgc ttcgaagttg ctgttgcagc
    10141 aaaaaaaact ttgggaaaat aaaaatgaac tgagagcgaa gctgcttaaa gatttacttc
    10201 aggaaagaga gcaaagggcc gagagataca attcgatttc aaattatctt gagcctaatc
    10261 tgaagtatgg tccaggaggc ctcagagaca tcgagcaagc cctccaagtt tatgatcttt
    10321 tttctgaaaa atttaaaaat cccgcacatg ctctgagcgt cttgaattat cacaaggcct
    10381 atatcctgac tctccgtcag aagcttcacc tgatggaatg cggggaagtt ttggtgagtt
    10441 cagttcaatc tgagcttggt cagtttttta attttaaaaa tcataaagac tttatgcgcg
    10501 atttagagcg gggtctttcc agaagccatt tttatgtaaa ttggattttc tccattgcgg
    10561 aaagtcctca gacgttgcta gcgaaaactc gcggtttgaa gtttaacaaa cctgaggatt
    10621 tgatttcggc gttaagacaa tatcctaatc ccttaggtca acagagagtc cgggaaaatt
    10681 tagatcgcct tttgcctgaa aaggtgagtg ccaagagcag tcgttggttc ggcaaagttt
    10741 tgcaagaaat attaagtccg aaagtttcca aagatttact gatcagtgcc tttgaatctc
    10801 gcctgattga taagatattg cctgccattc gtcatttggt cggttacgtc cagcatgatc
    10861 agtatcatcg ctacacggcg gacgtgcata tcatgcaggc ctgcgtgatg ttaaaggtct
    10921 tctataaaaa ccctaaagcc ttggggccct tgagcgcgtt tcatcgggag ctcaatgagg
    10981 aggactggaa aatcctttcc tgggcagccc tttaccatga tctagctaag ggactgcatt
    11041 cagagagtca tgcggattta ggtgtggaaa ttcttcgtaa ggattttaaa aaatatggtt
    11101 ttgcaaaaaa aataactgaa gaagtcgctt ggatcgtgaa atatcatctg gaactttcca
    11161 tcgccgcttt cagaaagaat ccaaaagcta agaagacttg ggaagacctt gaggccatcg
    11221 gcgcgaaggg ggctcgttta aaacgtctag ctgtatttac ggtcatggat atttgcgcca
    11281 ccaatccaga agcttggaat gagtggaaag gcttgctttt aagggacctg gtcgaggctt
    11341 tgaatgctcc gagtacgaag aactattttt ccttgaagac ttctttgcaa aagaaaaaat
    11401 taaagggtct ggaggctgtt cttgaagact ttgatgattt cctcctggaa agtctttccg
    11461 cctctgttct ggcggaggat ttaaaagccg ctcgtgaatc ttccgaatca ctttctccct
    11521 tggcctttac aaagggaaaa gaaatttggg tccgatttca ttccaaagaa gatcaagagg
    11581 gcttgttggc cgagtatgtt cagcgcttgt attcactcgg ccttggaatc cgccatgcgg
    11641 ctattcagac cctcaatggc gtgggagtct acgattggtt ccaagtgagc acgtctaaaa
    11701 atcctcaggt gatcgaaaaa attctgaaaa actcgcaggg attaatcaag gatagggcca
    11761 aggagtctga gaagaatatt ccaaaaaccc ttcccaaagt tatttatgat caaattgaat
    11821 gggtttccat tgacgacaat gaatgggttt taagttttag aggaagagat caagcgggat
    11881 tcctgacctt ggccacaaag accctggccg caatgagatt gaacattaaa tcggcccggg
    11941 ttcatacctg gggacgtcag gtgaatgata tctttatttt aaaaaaggcc cctcaaacac
    12001 cggaaatttt attggcagag atcaagaaaa gactgggtga atccagtctt tttgcataac
    12061 actcgctact tacaatcaga gcaattcaat tctgagtttt tttcttggag ccagatccgg
    12121 taatccccgt gtgattgcgc caattgtggt gagggtccgt ttttgttcgg ttgtaccaag
    12181 catcccattt ctttttaggt aatttgtggc tgacattgcc tttttcatca aaaatatcgg
    12241 gatctttgcc gaagagaaaa tccaatactt tagcgcctat gcccataaat accccgcgtg
    12301 ttgaattatt gctattttag ttatcgttgt tttataagtc aaagcaaggg cttaaagaat
    12361 caatttctag cggatatgca tcgcataatt aagagcgagg gttcgcgcag acatctataa
    12421 tatcgagacc ttgatcttca ctgacgtgcc ttcctctttt gtaatgtttg aagcaggcct
    12481 ttgcggccac ttcaaaagct tcgtcatagc ttaaggctga ggagctgaag acgaaagatt
    12541 cagctttcaa tttaaattta aactcgtagg actttgcttg aggagctttc tcaatccaag
    12601 caaagcttat tgaagaaata gccaagacaa gtgtcataac tagcgctttt ttcatacatc
    12661 ctccttaggg gactctaggc tttcttattt aagctgcaaa tcgagggcct aaagagttcg
    12721 aaataacttt ggcgatgcta cgtaaatatt tgatttaata aggtaaaaat atctcacaat
    12781 gaaacataat tgttgttgcc aactaggggg tgaaagtgac acttttggtg ctcgattgtt
    12841 caggctgtgg tcatgggggg cgccatgaat gtattcggat tatttttagc ctttgtcttg
    12901 agcttttcag ttcaagtgat ggcccgtgaa cggacattgg tgattgtcca ggtggaagac
    12961 accttgaagc tttctcacgt ccagaatttt tgggactctc ttaattacaa cacagataca
    13021 aaacaaagat tcattggtat gaatgccgct ttgagcgagt tggcgcgaaa gaatgcggct
    13081 agcacttttg cctatttaac ccaaggtcct gagatggcgg ccggcgaggc cgagatggag
    13141 tttataagcg taaacggttt tccccggggt cagtttgtac cttatagtaa taatgccgat
    13201 agagacaccc agatgaaggt gttgcgaaaa ctcattaaga cgtccaatcc agcgcgagtc
    13261 attttgatga gtcacaatgg gggactcgat cctgaaatct ttcacggcat cgctatggaa
    13321 tttaaagata tttatttttt gcagtatctc cacgttattt actcaattac ttctcctaca
    13381 gaaatgggaa gttcgctttt tttagagcag acggggtttg tgaccgcagt agagctttta
    13441 gtggattggc agctcaaggg cttggtggga cttaaggaaa caatcactct agcccaactt
    13501 ctgatcccaa agattgttta tgaaaatttg gattcgccgg ggatcgtgga gtatggagtc
    13561 ccgaggtttg tgaactgtga tgatttccaa tggaaatggg tcgctaaaaa tgactattca
    13621 tttttagttt cactgcgaga ccatcttatc gaacgttgtc atatgcaaat ctcgcctttg
    13681 ggatcgccag ctcattaatg aaattaaaat atcagcttcc ccgtatttat gaagatcttt
    13741 taccccgagg cttattgcaa atagaaccgc tggaaactaa ggccacctgt gatcagtgca
    13801 ttatggcaag gccacagaat aagggtaaaa ttttttatac tcccgacctt aagtgctgca
    13861 ctttttatcc ctttttgcca aattatattg tgggagctat tttgacttac gagacttcat
    13921 cagcaccttc agcggtagcg gttattcgca gtaagattgc aaaacgcgag tacagtttac
    13981 ctttaggtct ggtggcgccg ccaaattatc aagtgcccta taatcagaga cggcaaaatg
    14041 aatttggtca aagagaagat tggctttgcc cttattttaa taaagagcaa cagaactgcg
    14101 gtatttggcg tcaacgaggt gctgtttgca caagttttta ttgcaagagt tcttacgggg
    14161 ttaaaggttt gaaattttgg gatgagcttt caaattattt gacctatgtc gagatggctc
    14221 tcatggaaga atcattagtg atgttggatt tttctccgcg ccaaatcagt gatttgctca
    14281 attacctgaa ccgtcaggaa gcgaccaaga cagaaatgaa aagctcgagg atgcccgaag
    14341 aaaggtctcg gagactttgg aatggatact atgaagatca agaagctttt tttaaaaaat
    14401 cgtttgagat cgttaagaaa ttagataaaa actcatttcg agagttgctc ggtgagcctg
    14461 gtaaacaact tgaggaacgt ttacagaaag cgtttgagaa aatatgtcca tgattgaaga
    14521 gcgccaaaaa caaatcatta gcgagtttga aaatttgaag gattgggaag aacgctataa
    14581 aaaaatcatt gagatgggtc gccaactcac ggaaatgcct gagaatttaa aaaccgaaga
    14641 gaataaagta aagggctgcc aatctcaagt ctggttgttc gcgcaactca cccctcaagg
    14701 tgagatgatt ctacagggag atagtgatgc catgattgta aaaggactcg tggctttact
    14761 cttgcgtgtt tattcgggag ccaatcccaa tgagatttta aaaaccccgc cggagttttt
    14821 aaagactcta ggttttgaag gtcatctttc tccgagtcgg agcaacggtc tgcatgcgat
    14881 gcttaagcaa attcgttttt atgcaaccgc ctttgcctac gtcttgaatt cgtcaaaaaa
    14941 atcttaatag ttttgactgt tggcacttct gttcacagaa tagaaaaaat gcatcatgca
    15001 ttatttctaa aaacaggagg attttggtgg tcaagtcggt tctcttagtt gccattggtt
    15061 attttctttc gcccatagca attgcaaaaa ttcaagacag agttatttat ggcgacgaca
    15121 atcgtgtaga gttttttcaa gtgaccagag cggatgttcg cgaggttgct gacagtacgg
    15181 ccgcattgat tcgcaactca aatttagagg caaaaggtga tggaaatacc accgtgaaag
    15241 cggagacctt tcaaaaattt gcgggacttt gcagcacaga gcgttttgca gcagagcctt
    15301 caggtgcttt ttgctcagca tttttagtgg ccgatgatat ggttgccact gcaggccact
    15361 gtattgatgc gtctcgatgt ccccaagtga gctttgtgtt ttcttacaga atgtccgagc
    15421 aaagtcaggc gccagttgag gtaaacagtt ctgaggttta tcgctgcacg caagttttag
    15481 aaagagagca aaccggcgcg caggattatg cgctggtaag attagatcgt tccgttcgtg
    15541 gccatcgtgt tttaactttg gcaaaatcct cagtaaatgt aggggataac ttaattacag
    15601 tgggacaccc atcagggatt ccaacaaaag tgaccgataa gggattggtg cgcagtgtga
    15661 aagcagagtt tttcactgcc aacttagata cttacggtgg aaattcggga tccgcagtat
    15721 ttaatcaaga caccctcgaa gtggcaggga ttttggttcg gggagagaat gactttatct
    15781 acgatagcgt aaacaaatgc tccgtgagca atcgctgttc ggcggaaggc tgtcgcggtg
    15841 aagatgtaac tcaaatctct tatatttcag aagcacttaa aaagtactag aaaatcaaat
    15901 tgtaatttat ggaatttcaa cgccccgaca aaggggcgtt gtcgttttca agcaactaaa
    15961 gagtcacgtg gactttgtga gaatcatcca ggtcatcgag gaagttcaag aattcttcga
    16021 cttctttgag ttgagcttcg tccaatacgg tcggagtttt tgctttataa gaaagctctg
    16081 aagtggtgat tttccacccg cgtttagtca gggcttcgtt tactttaatg aggtcgttat
    16141 ggctcgtgta aaactcataa gctccatcat cgtctttata gacttcgttg gctccggctt
    16201 cgatggcttc ttcatccgga tcaaaggttc cggcttttgt tccaattaga taccctacgt
    16261 gttcaaacat ccaggcaacg cttcctgttt cacccatatt tccatcgtgg gatttaaaag
    16321 cgtggcgcat ttctgaggcg gttctatttt tattatcggt ttgacactcc acaatgactc
    16381 ccacgccatg gggtccatag ccttcgtaaa ccacttcctc aataattttg ccgtcatcaa
    16441 gtaagcccgc gccctttttg atagcgcgat cgatagtatc attggggcaa gacatttttt
    16501 tagcatcatc gatagctagg cggagacggg aattgcctgc ggggtcaggt ccaccagctt
    16561 ttgcagccac agaaatctcg cgagcgagtt tggtgaatat ttggcctttt tgcatggcct
    16621 tttctacttt tccggcgttt ttccatgatt ttcccatagc agccatgaaa tcaaattcgg
    16681 aaatatttgg caagagaggc cgcaggatgt aaaaaggccc atgttttctt tagaagttcc
    16741 agatgtatgg aaaaagatta aaaatattca aaaatcctgt gaatccttgc tcgaagggga
    16801 ttcttttatc cttcccccat atcccgcaag ggacgtggag gtcttagatt ccaagctcca
    16861 ccctcctaaa gtgggttttt caaaaattga ggggcaggcc cgcatgctcc atgatttagc
    16921 aagcatcgaa ctccaggcca tggaaatcgg gctccgcact ttggcggagt ttcccgaagc
    16981 gcctccagat tttcgcgaag agttggtgga ggtgacggtt tcagaggcca atcatttgcg
    17041 tttatgtttg gaaggtatcc aagaccttgg ctttaaatgg ggagattggc ccgttcactg
    17101 tgccctttgg gatgctgtca gcgccgaaga ctccctttta gatcgcatcc tgatagtcca
    17161 tcgctacctt gaaggcagtg gtttagatgc cggcagttat ctaatgcagc gactcgatgg
    17221 cgtgcgcgat ggaaagctag ctcggcaaat tgtcgggcaa attaatttcg aagaaatcgg
    17281 tcacgtaggg tttggttctc agtggtatcg aaaaatttgc caacttgaga agatcgatgc
    17341 gaccgaagat tttcctcggc gtatggaagc cttgcgcatg aagttgccta agcggatgga
    17401 aaagatcaat cgagagcttc gccgacaagc cggttttacg gagcaggaaa ttgattattg
    17461 cgaagtctat cgcaagagta ttttaccgca gaaataataa taacaattcg ggatctttct
    17521 aaaaatagtg gggaaaatgt ggagaaggga cctaggcctt taacaaaaaa aagttcctgt
    17581 attccggctg catttccgtc gcatttcagc ggcacttcta aagggatttg agtccccatc
    17641 gctgggcttc ccttctattg caaagaccac agagggcgcg caggttcttg atctcatcgc
    17701 tccctccaca ggccagaggc ctgacatgat caatttgcag ttgaaactca gagccacatc
    17761 ttttccctgt gagggggtgg gtatattcac agcagtgctg ggctttttca aa
//
LOCUS       DATBTE010000367         3460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2441562, whole genome shotgun sequence.
ACCESSION   DATBTE010000367 DATBTE010000000
VERSION     DATBTE010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2441562"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1073)
                     /locus_tag="VIG33_13775"
     CDS             complement(138..1073)
                     /locus_tag="VIG33_13775"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8271954.1"
                     /translation="MDIKTLNEILEFVEAPYRVTSPIVESGQRKVYYAQDIHSSAQVV
                     LKVCPLYPVMVARIKREIRILQEIDSKYFPKFFYEFFITDQELSYFVDSLDPKTQQHR
                     LVEIAKMGIKPFLVTAEECIEHIHWDKCWENLRAEPVFITFLIHLFSGLKLLWDKKIV
                     HRDLKPENILIRPNMEPVIIDLGIAKSMNKGATIITNPAFSSPCTPRFAAPEQLTNNK
                     AEVTYKSDQFSVGVIAFLVLAGKLPYGSDEEVGMEGVIRNFFQGKIEDLRRHNSEISE
                     NFVLLIERLLKVVPYQRFRSVEEIISALTALEETK"
     gene            1860..2942
                     /locus_tag="VIG33_13780"
     CDS             1860..2942
                     /locus_tag="VIG33_13780"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HEY8271955.1"
                     /translation="MDTRFGLRHPQIMNWNQITYPKPIQKGSTIGVTAPSNGVKPKHE
                     ARLNLALKHLELRGFSYLEGKCLRDDKKSVSANAQQRAEDFTSFWLDPSVDMIFPPWG
                     GEFLINILGRLDFKELVKNRAKWVQGYSDTSTLLFALTTITGIATVHGLNLMDSILEN
                     DDSLTSKSFDWLTLSTGARFEQTSSPRFQTEFEDFSKNVHAKFKLEHNTFWKNFKGGD
                     EITLQGRMIGGCLDTLRNLVGTPYGDLPRFASRFPDSKVILYLENAGSTPAEVCRTLW
                     NMRFAGWFEVASGVVFGRSAVPDNSNSEEFSYEDAIEDALASTGLPIILDADIGHHPP
                     QMTIINGSMAQLESSNGRGRLVQTLI"
     gene            3215..>3460
                     /locus_tag="VIG33_13785"
     CDS             3215..>3460
                     /locus_tag="VIG33_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271956.1"
                     /translation="MSFILVAAAGPLTKIRPFLINQLLMSRHLRVPTVISGGAGFERG
                     QFHRFTGPMGTIVTGLKWDQRLKCQARSSALMVNQTDA"
BASE COUNT          950 a          756 c          738 g         1016 t
ORIGIN      
        1 tttcaattca gtagtttgct cttcaatctt tttcttcgat atgtggacgg ggctaaaaat
       61 aaccccatct ccacaatttt cttccttatg gctatcaacg ctccaaacag tattgtgccc
      121 ggcttggtga taaattttca tttcgtctcc tctaacgcag ttaaggcaga aataatttct
      181 tcgacgctcc ggaatctctg ataaggaacg acctttagaa gcctttctat tagcaaaaca
      241 aaattttccg atatttcgct gttatgccgt cgcaaatctt ctatttttcc ttggaaaaaa
      301 tttcgaatca cgccctccat cccaacttct tcgtcacttc catacggcaa ctttccggct
      361 agaactaaaa aggcaattac accaactgaa aactggtctg acttataagt aacttctgcc
      421 ttattattgg taagctgctc tggcgcggca aacctaggag tacacggaga ggagaatgca
      481 ggatttgtaa taattgttgc tcctttattc atactttttg caattccaag gtcaataatt
      541 acaggttcca tatttggccg aataagaata ttttcgggct ttagatcgcg atgaacaatc
      601 tttttgtccc agagaagctt caatccagaa aataggtgaa tgagaaaagt tataaatact
      661 ggttccgctc gaagattttc ccagcattta tcccagtgaa tatgctcaat gcactcttca
      721 gctgtaacaa gaaatggctt gattcccatt tttgcgatct cgacgaggcg atgttgttgc
      781 gttttaggat cgagagaatc aacgaagtag ctcagctctt ggtcagtaat aaaaaattca
      841 tagaagaatt ttgggaaata cttcgagtcg atttcctgaa gaattctgat ttcacgcttt
      901 attcttgcaa ccatgactgg atatagcggg cagaccttca gcacaacctg agcggaagag
      961 tgtatatctt gtgcataata caccttcctc tgtccagatt ctacgattgg cgaagtaact
     1021 cgataaggag cctcaacgaa ttccaaaatt tcattcagtg tttttatatc cattcgagag
     1081 cctgtcaggc tttaacccct tgttctaaaa tttgatgaat gagggccgat aaagtatttt
     1141 tttcgctcgg cattttcctg atattggcca gcagtgatgg cgacactcga atagcaatca
     1201 aaaatttcgt ttctcacgtc ttaggccttc cagctacttg tgtttttatc attttctgct
     1261 tggcggactc tatctgggtt tgtatagttt tcttatatac gaaaactaca ttggttagtc
     1321 aatttactgt ttactgcttc tagatataaa taaatttatt ttatgaggcg gcatctttga
     1381 agttcatttc gccgaaagat ctgcgacatt agagacaacg gagaccacat gctgagtcga
     1441 ggatatttag ttggaaattc aattgacgaa cttgcggttt tatcagttca agcccagatg
     1501 agagcgaaga tagggttttt acggatattc acatttttgt cgaaaacttc tttcgtgatc
     1561 taattgaaat aattactggt accaaattaa aaatgcaaat gttgaccgtt gtaatacacc
     1621 cggagttgcc ccaaaatccc aggtttcagg cttactcctt gggaaggttg ccgaccccat
     1681 catatacagt gaggatacct tgcgcccacc tccgacggcc cgaaaaaccc ccgaaatcaa
     1741 ctaagcgaac cttttggggc aaattccaag ctcggtagcg gtatggatag gaagccgtca
     1801 gagccgcttc atagggctta aatggaacta accgccagtt tctttataag attaagataa
     1861 tggacactcg attcggctta aggcatcctc aaattatgaa ttggaatcaa atcacttatc
     1921 caaaaccaat ccagaaaggc tcgacaatcg gcgttactgc gccatcgaat ggtgttaaac
     1981 caaagcatga agctcgtttg aatcttgcgc tcaaacatct ggagctgcga ggttttagtt
     2041 atctcgaagg gaagtgtctt cgcgacgaca agaaatcggt cagcgccaac gctcaacagc
     2101 gcgctgagga tttcacgtca ttttggctag atccgtctgt cgacatgatt tttccccctt
     2161 ggggtggcga gtttttaatt aatattttgg gcaggcttga tttcaaagag ttagtaaaaa
     2221 atagagcgaa atgggttcaa ggttattccg acacgtcaac tctcctgttt gcgctaacta
     2281 ccatcaccgg tattgcgacc gtgcatggtt tgaaccttat ggactcgatc cttgagaatg
     2341 atgattcttt aacgagtaaa tcctttgact ggctcactct ttctactggc gcacgattcg
     2401 agcaaacatc ctctcctcga tttcaaactg aatttgagga cttttcaaag aatgtgcatg
     2461 ccaaattcaa gttggagcat aatactttct ggaaaaactt taagggcgga gacgaaatta
     2521 cgcttcaggg aagaatgatc ggcggatgcc tggatactct gagaaatctt gtgggaactc
     2581 cttatgggga tctccctcgg ttcgcatcca gattccctga ttccaaagta attctttacc
     2641 tggaaaatgc agggtctaca cccgccgagg tttgccgaac gctttggaat atgcgatttg
     2701 caggttggtt tgaagtcgct agtggcgtag ttttcggacg atctgctgtt ccagataatt
     2761 ctaactcaga agaattttct tacgaagacg caattgaaga tgcgctagca agcacggggt
     2821 tgcctattat cttagacgct gatatcggac atcacccgcc acagatgaca ataatcaacg
     2881 gatcgatggc gcagcttgaa tcatcaaacg gacgaggcag gcttgttcag acactcatat
     2941 aacacttgtt ttcgttaatt ctcacgatga ggaacgcaca ttggacctgt tcaatactca
     3001 gctccaaaac tcgtatagaa aaggctgcct attaaatcgt tcttttgggc tttcaaaaaa
     3061 gcccagcaag atctttctga tctgtgacta gacgaaagta ctatctgcgt cactccttca
     3121 gaaatcgggc caaattgccg aaataggact atgaagttgt tggtcataat ttccctcttt
     3181 ttgctttaca gccgagcctc agtggcggag aacattgtcg tttattttgg tggcggcggc
     3241 ggggccacta acaaagatac gaccattttt gatcaatcaa ttgctgatgt cgcggcattt
     3301 gcgagtacca acggttatca gtggaggcgc cggttttgaa aggggccaat tccaccggtt
     3361 taccggtccc atggggacca tagtcaccgg tttgaaatgg gaccagcgcc taaaatgcca
     3421 agcaagatcc tcggccttga tggtgaatca aaccgatgct
//
LOCUS       DATBTE010000368         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1894834, whole genome shotgun sequence.
ACCESSION   DATBTE010000368 DATBTE010000000
VERSION     DATBTE010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894834"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..425)
                     /locus_tag="VIG33_13790"
     CDS             complement(189..425)
                     /locus_tag="VIG33_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271957.1"
                     /translation="MRLNEKAAESLNITEVIESELMAGSSMAEHRSYHELEDSPILEV
                     DALTELKANLQVLTDLTSRLSFLSREIRYMMKID"
     gene            complement(566..1210)
                     /locus_tag="VIG33_13795"
     CDS             complement(566..1210)
                     /locus_tag="VIG33_13795"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEY8271958.1"
                     /translation="MRKYLTFLCVLIAFGLQACAPKSQEDCGFVQNVYGERISWKGEV
                     PVILYLHESVPEEYVSAIESAAKAWQDATGKRVINIVTSQRISGPLNSQKDGRNVIYY
                     SNIWEADKKSEQARTSIYWVGDQIKEADMRINAAKDMNGTSYFKYYWNPPGVAATAVA
                     NAVNIEALVIHELGHVLGLKHKDTNGSVMATYLANNTDRVNIAATDHSALQCEY"
BASE COUNT          448 a          369 c          298 g          499 t
ORIGIN      
        1 cgctacgatg gcggccagga aggccttctc atggaaggag gcactcttta gttatccctc
       61 actgctgtga gcaaactctt tctaattcgc aaaatccttt aagggatttc attatcaaca
      121 atatggaggg ccagtttcaa aaagttatgc ctggaaaatt cttgggaggg attgtgaagt
      181 ctgaaggatt aatcgatctt catcatgtag cggatttcac gactcaaaaa agacaaacgc
      241 gaagtaagat ccgtcagaac ctgtaaattg gcttttaatt cagttaaagc atcgacctca
      301 agaattggag aatcctctag ctcgtgataa gaacgatgtt ctgccatgga agaacccgcc
      361 attaactccg actctataac ctcggtgatg ttaagacttt ccgcagcttt ctcatttagt
      421 ctcattagca gtactccttt ttcatttgca cccaatcatc tagtctagtg acaatatatc
      481 tcgctctaca acttaaaaaa tcttacatat agtatttaca attcacgaca ctttaaactc
      541 accatctatt attcacactg tagagctaat actcacactg caaagcagag tgatccgtcg
      601 ccgcgatatt tactcggtcc gtattattag ccaaatacgt ggccataaca cttccatttg
      661 tatccttatg ttttaagccc agcacatgtc ccagttcatg gataacaagc gcttcgatgt
      721 ttacagcatt cgcgacagcg gtcgctgcga ctccaggtgg attccagtag tatttaaaat
      781 aagatgtccc attcatatcc ttcgcagcat taattcgcat atcagcttcc ttaatctgat
      841 ctccaaccca atagattgaa gtgcgtgcct gctcggattt tttgtcagct tcccaaatat
      901 tagaataata tatgacgttt cggccatctt tttgagagtt caaggggcca cttattcttt
      961 gacttgtgac aatgtttatc acgcgcttcc ctgttgcatc ttgccaagcc tttgccgcag
     1021 actcaatggc actgacatat tcttcaggaa cagattcatg caaatagagg atcactggaa
     1081 cttcaccctt ccaggaaata cgctcgccgt aaacgttctg aacaaatcca cagtcctcct
     1141 gggatttggg cgcacaagcc tgtaagccga acgcaatgag cacgcacaag aaggtcaaat
     1201 acttccgcat gaatagggtc tcctcctcat ttgtctatca agtctggggc cagctatttg
     1261 atttccagaa gtgttcgtag gcacctgaat ttactagatt ttttttgttt tctggttttc
     1321 tgctttttct acaatgtact gtttatgtta tggtgagttt cacacaaata acttaactga
     1381 ttgattttat taataaaaaa acatgttcac tttccaacag ggaggggccg tgaattgttc
     1441 taagaatttt gatgaataag aatttttgtg gtcttccata agtttgtctc tagagacaga
     1501 aacaccctta ttttttagct ccccctgatc tcgaccaata gccagaatga cactacgtac
     1561 ttaattcctc gaaatcattg agaatctcat tttggtacag cccttgctta taga
//
LOCUS       DATBTE010000369        15110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1895126, whole genome shotgun sequence.
ACCESSION   DATBTE010000369 DATBTE010000000
VERSION     DATBTE010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 15110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15110
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1895126"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VIG33_13800"
     CDS             <1..687
                     /locus_tag="VIG33_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY8271959.1"
                     /translation="YALEADSGRQVWLYSRQDTSNFSIRGGSKPALKDGNLFVGFSDG
                     TLVSLNAKTGNLQWELPLNKNKKFRDVDASPIIDGDLIYISGYDDKLYCISAQKGDVQ
                     WKVDGGGYYPITIYNDKIYYSTTTGEVWALQKGNGQKAWAYQLKEGIATQVKIYKGLA
                     VFGESQGQLKFLDANTGKQISSFDPGRGIMSTPLVDDKNNHIYFISGEANIYAMEAKW
                     SYPQWIPYLQ"
     gene            696..1109
                     /locus_tag="VIG33_13805"
     CDS             696..1109
                     /locus_tag="VIG33_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271960.1"
                     /translation="MSQWIYASILSLTLFGCATGKCVNAASPPPKSAPVSTPAPTPHS
                     GEIAMKPESKLDKVKVYKPDGSLQCGQGKAIPIAEMQKELKDIKVYSSMNKNDGMMRI
                     QLCGSPTGNANVYEIDRKQLQAAIKLGFKEWTFDN"
     gene            complement(1168..2571)
                     /locus_tag="VIG33_13810"
     CDS             complement(1168..2571)
                     /locus_tag="VIG33_13810"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HEY8271961.1"
                     /translation="MQKLFIFILLSTVSLKAFSQERLQYTFADPQKMQLIDSGVAALQ
                     KRFEMIATAKKTIDIEYFIYNTDKVGRLFTQALIEKAQKDHIKIRLLVDSSIAVFQLK
                     EDYAAVIQSAVKEGLLEIRYYNKTSFFNVVGAQFRSHRKSLIIDGENVDGQGMTGGRN
                     IAEEYFDLSPTYNFLDTDLYVQGTLVRAMKKSFEDFWESGLTVAAPKTPSADSKSVQK
                     AKEFITSKPADAILLKKINRSGRQMLKTQVTSVCTDSSFVADGIDKSPNSPVYRAAIQ
                     EILAAQESVAIESPYFVVSYDGLDFLKDVMNIGQKVKLQLLTNSLNSTDATYTSAALY
                     PYMSIWRNLGIRLHLYRGNPPQNYPTPVAEALHTRWGTHSKRAVMDGSTILVGTYNAD
                     PRSKDINVEMAVVCRHNKVLGKAIQDNFRARLKQSIEINRLGLPADGRVFSDVGLLKG
                     LQFGLFLIPANLFSFLL"
     gene            2728..3216
                     /locus_tag="VIG33_13815"
     CDS             2728..3216
                     /locus_tag="VIG33_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271962.1"
                     /translation="MAKALGLLAILISIYGLAKAGVGDKDSDLCFEVKDSQKFLKVNT
                     RVVPQGTPPEIEKIQELEKSVLKFYGSKCADVGKSFPLEKFFSAAYGNCFDKCKTITY
                     TDKDLNEGKAASGYFGDFCRSQCTAYNWQASAWIEGKKEGEKSCSAGVKTNIQSPASG
                     KR"
     gene            complement(3234..3605)
                     /locus_tag="VIG33_13820"
     CDS             complement(3234..3605)
                     /locus_tag="VIG33_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEY8271963.1"
                     /translation="MHARKPKLNFILSLRFFLSEMNLLIRSPVFLLLTLLGNFLIGIF
                     CVVFYFLEHEINPRIHGFIDALWWGFATATTTGYGDITPVTFQGKCLGILLMLTGMAL
                     FAMFTALFAETILASSKQYKV"
     gene            complement(3635..6565)
                     /locus_tag="VIG33_13825"
     CDS             complement(3635..6565)
                     /locus_tag="VIG33_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY8271964.1"
                     /translation="MNYNLNYTLKIFSLSFFLMLNPHAQGAPLAEAPVQENIFVDRPN
                     ILVKSAHQITFEGPKSGEGYFSADGKKMIFQSERYPGNPFYQMYVMDLETGKTDLVSS
                     GKGKTTCGWIHPSLKKLLFSSTQLDPQWKKKQEEEIESRKKPVKGRYAWSFDENYDIF
                     TSDLKGKNLKQLTKSLGYDAEGSYSPDGKWIAFASNRAGYTEKISGEDQKLFQQDPSY
                     MMDIYLMKENGTQVKRLTDAKGYDGGPFFSADGKKITWRRFSSNGATAEIYTMNVDGS
                     EQKQITHLKSMSWAPYYHPSGDYIIFASSVLGYANFELFIVDSEGTKPPVRVTFGEGF
                     DGLASFSLDGKQMTWSHRNEKGESQIYIADWDDAEARKLLGLPEKDLSSTDLRPQITA
                     SDAQSIVNYMASEKLGGRKVGGTEEKIYTSKLADIFKSFGLKGGANDGSFFEKFEFTS
                     GVKLGTENKMEFVGNFKKNLQIEKDYSVLSFSKIGRIAESPVVFVGYGIKAPATDKFP
                     AYDSYKNAKVQRKWVVIFKDIPQDVAPGLRHHLNTYARLQHKITIARNEGAVGVIFVD
                     PTQEKEDLSKLKFEGTLSDSSLAVLKIQQGPLKELLKSAGQDFNSLLGKLNQGEALEA
                     ISIPMIYLTATVSLDFERSQGISVVAKLPVKGAVTGLLLSAHGDHLGRGEIGNSLAKA
                     EERGQIHFGADDNASGVAGILELAHYFGDQYKRKPGSLKKNLYFAIWSGEESGLLGST
                     AFVKGWGKSHKQKISNYFNANLNMDMIGHLQERLYVQGVGSADHWSQLAEEISIRQGV
                     PLTLQEDPYLPTDSTALYMAEVPAINFFTGSHENYHSPRDRAETLNYPGLERVIKTVA
                     DYTRLLADSSVKVLKYVKVGGNPNKQLEGRSFRTYLGTIPDYTQEGVKGVRVSGASKE
                     SPAAQAGIQENDVIVEFDGIKIENIYDYVYTLQAVKPNVETVMKVKRGDQIKELKITP
                     KLKD"
     gene            6595..7284
                     /locus_tag="VIG33_13830"
     CDS             6595..7284
                     /locus_tag="VIG33_13830"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEY8271965.1"
                     /translation="MGSRMKELPLKRILFGPFIICLLMLTACALQKPQTASPAAPTTR
                     DRNFFKSPVTTENEKVEFIAHTENKPMKAYLSMNLPYPPYQVLFEQIQKAEGIALTNR
                     GEAHMTVITPVEYDKVLKKHLNIAEIHKIAEATKIQELPFTPVCIGKGQKELKGKLEK
                     VYFVVVESPALIELRGQIEEAYIKKGGKPQEFVPERFFPHVTLGYSARDLHYEDGVLK
                     NNKSCLYQFQQ"
     gene            7314..8474
                     /locus_tag="VIG33_13835"
     CDS             7314..8474
                     /locus_tag="VIG33_13835"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEY8271966.1"
                     /translation="MQIDCKYKKECSGCQYLDLSLQEQQHLKTQALSGLLRDFKIPFK
                     SAINLTSLGPAWLRDRVDLVFNSVGTEGVLGLYKKNSREILDIEQCQQLSPQLQAWLS
                     EIRKIHWPIRKGSLRLRVGPQRQKGIWLDFANIDIKNLLEEKTILSNLLNCAVVEIGQ
                     KHKVLQFVNGRLKLKDAEPQVWFQSFIETQAVDLFCSIGNFTQTGIQANRDLTKVISQ
                     WLEEIHSQHILEFGSGIGNLSFPALGYARKLTACEIDRGALAGFEKTLQKLSQVPGFE
                     KIRERVEIQQGDFQNRNPQKFAEYDTVLVNPPRSGLKEFLNPLITETKKPKYFLYMSC
                     FPESFVLDGQRLQQAGYSLQKLNIVDQFPQTTHYEILSLWGLNEEKKVSGHK"
     gene            complement(8440..9015)
                     /locus_tag="VIG33_13840"
     CDS             complement(8440..9015)
                     /locus_tag="VIG33_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271967.1"
                     /translation="MRIEKVKPENPNLPWEIPEDKVPVPSEYPESLKKLFRNRQNLQW
                     EMDTGFQSGPIGKRKGYTLTLWSLMASTIDALLLIAMNCVFLMAFIKIIKVPIDQGLL
                     KDFALIYLGAAWLYMITTRFFIGASIGEAACDIRLGEPRERLSPWYFLRVVFRATLIA
                     ATGVVVLPLLSLILGRDIPGIICGLKLFSLH"
     gene            complement(9026..10939)
                     /locus_tag="VIG33_13845"
     CDS             complement(9026..10939)
                     /locus_tag="VIG33_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271968.1"
                     /translation="MRWMTVALSLIKMTAVNVAVFTMVASPLAQGAQAKAVATANVKA
                     KLQAKASDYNYNIDKKSIQEFLKYSGLNKRKLTVGEFYSKMRPFYPKTLRGEMDQWVQ
                     MNRNEFMPEVEASTIKDSDGKERVRLLITKDGQTVTATYNPDNNAKFLKVNNVFLTKD
                     DMKFHEQAIRKLILGDKGIKAAVLKAPRKTRLNQSVALSYGEFKRLKPRQRAEYLVRL
                     RYVMEDAQQVMEKYQGSQAANEMRHDFFVQWLLLGGSAEAKGGKASKSIRGAVSGDPC
                     IVSGYIAKYGNDSSCGGSGPGREALIQEMNTYGGGQCMNGSVSCNPLVYGYRENGAAI
                     CVKHGKNQEIQTSTSQSCPNQSPLRKGTPDEANDKKRIIESYLKANGTTDAEKNINLL
                     FDKDGKLSQAQYDLIKAHLDKLNTYIDRALEFCSRPPLGDIRKIRDEQESACRALETR
                     KMEVLTYPETPLPPIVTPEKDCSLEKPGSTLVDGVCVCAPGSVVQTITEDNVERQACV
                     PAEAIPADDTKKECNKDEERDDQGNCVAAGACTWCKWLIGGAIFAAVGTALYFIFKDD
                     DDDDHNPQPSVDPCPPAPLICLPPGTPTTPATPPGPPPIQPPPTDPIPPPAVTPFVES
                     TNGNSNSTSGGVR"
     gene            complement(11034..11768)
                     /locus_tag="VIG33_13850"
     CDS             complement(11034..11768)
                     /locus_tag="VIG33_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY8271969.1"
                     /translation="MSQEPSSDLKQPVGIYILAVLFMAAPLGNILVSFAGSGVLNWYY
                     PQEFVELLKTIPVIDWLWLAGIFISGFALLLKHKMTWLLAVVSLLIVLGMNTYRAFTI
                     DETVLNPEFVRVQILLSILVTFSVLIIAFYARYPYLDRRQQWMFPTAHRYDVRTPVIV
                     HTGGDLAGLTESVSTAGIRIRLAKKAESLEGKNEVEFSLSELPDFKVVKAEVVEFNGD
                     VLRLKYKNFGWGRRGYLEAWLKSKKI"
     gene            complement(11876..13366)
                     /locus_tag="VIG33_13855"
     CDS             complement(11876..13366)
                     /locus_tag="VIG33_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271970.1"
                     /translation="MKNKSNLSTVLVLGVLYLSACAPKNFEAVQQVATDTTKAIGCND
                     FESKTWDAINGYLIEQKSMLSLEELKNPLRSSLKTVKAPSGNLNERKVEALAQDMNEL
                     YNILLNEVPQVEEVQDAQGLLEVLSALELGDHTTDAKVRLQNKIEAQFAKIKKQIAAM
                     DVQCATPVAPVPEEGEGAATPEFVKTSLPRPVFGIRFTFATAYQSCQVLDEPIMTKNT
                     QDIDDRAITITGKHPNGVGNRREISNVTALLHSHPYYKNINSYANGCLKASTYPMIYD
                     YGGKPYATTSANSVLNFFRDGGNGTKVLGTDCSGFVYSGLAAAGLRLSPNKVVKATGV
                     NGISATMYMDPPRNGLNCLAKITVTPKSDLKAGDIVAVHGHVIMIDSVGSDPFGINGV
                     KNADACSRLEPKDFDFVVIQSSPSKNAVGMNRYQAKDYLYESDKMRTGLTQYAYYACL
                     ARLNNRNYTPNLGTLSVIRHRQTPECLGARIKLSQESCIQSCPQLK"
     gene            13470..14528
                     /locus_tag="VIG33_13860"
     CDS             13470..14528
                     /locus_tag="VIG33_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEY8271971.1"
                     /translation="MNEKFIKTQVLYNLIDWEPFILLACLLAITWIFYKFFLQDASPE
                     RHISIRSQFQALIRHFFIFTISFVAFISLHSITGGDFVNFARITPYLALTTFAWGSII
                     FVKTCRLLVLQYLFLGSMRAGVPVLLVNIFSLLLSIILTVWALSQIFGLDLGPLMATS
                     AVFSIVLGLALQDTLGNLFAGISLQIDKSYEIGDWLEVVQGIQRATGQVKEITWRSTN
                     LVGLSDEEITISNRAMANAQISNFSPQGHPIVRSQVFRLPYGCNLEKAKELLERAAVE
                     ISDIRGLPAPFAFVQETTENWVGMKMIYFIDNFGSQYSIGDKVLRKGIDALRTHGIEL
                     ARNVIEVHQKSIDNEQTR"
     gene            14512..>15110
                     /locus_tag="VIG33_13865"
     CDS             14512..>15110
                     /locus_tag="VIG33_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8271972.1"
                     /translation="MSKPVNLNTHLNIDLNADLKTDFESPLATKIRKQIVQALNDFDL
                     IHDGDKIAVAVSGGKDSSILVALLNEIRKRSERKFTIEALILDQKQPGFEIRAFKEWI
                     ESLGIKLHILEKDTYSIVKEKTPEGSVYCTLCAKFRRAILYDFTHAHGFTKMALGHHR
                     DDLIETTLLNMFYIGQMGTMSPKLKSDDGRNIVIRPLAYV"
BASE COUNT         4236 a         3507 c         3140 g         4227 t
ORIGIN      
        1 tatgctttag aagcagattc aggccgtcag gtttggctgt attcacggca agacacctcc
       61 aacttctcta ttcgaggagg ctcaaaacca gcgttaaagg atggcaactt atttgtgggt
      121 ttcagcgacg gaactttagt gagcttaaat gcaaagactg gaaatttgca atgggagctt
      181 cctttaaata agaataaaaa atttcgcgat gtggatgcct ctcccatcat tgatggagat
      241 ctgatttata tttcgggata cgacgacaag ctctattgta tcagcgccca aaaaggggat
      301 gttcaatgga aagttgatgg aggcgggtac taccccatca ccatttataa cgacaaaatt
      361 tattactcca caaccaccgg tgaagtttgg gccctgcaaa aaggcaatgg ccaaaaggcc
      421 tgggcttatc aattgaaaga gggcattgcc acccaagtta aaatctacaa gggactggct
      481 gtttttggcg agtcccaagg ccaactaaaa ttcctcgatg ccaacactgg caaacagatt
      541 tcttcttttg atccaggacg tggaatcatg tccacgcctt tggtggatga caaaaacaat
      601 catatttatt ttatctccgg tgaagccaat atttatgcga tggaagccaa atggtcttac
      661 ccgcaatgga tcccatatct gcaataggag ctaagatgtc acaatggatt tatgcaagta
      721 ttctgagttt aactctcttt ggatgtgcca ccgggaagtg tgtgaatgca gctagccctc
      781 ctcctaaatc tgcgccagta tctacgcctg cgccaactcc tcattcagga gagattgcca
      841 tgaagccaga gtctaaatta gataaagtta aagtctataa accggacgga tctcttcagt
      901 gtggccaagg caaagctatt cccattgcag aaatgcagaa agaattgaaa gacattaaag
      961 tctattcaag catgaataaa aacgacggta tgatgcggat tcaactttgt ggatctccca
     1021 cgggaaatgc caatgtgtat gaaattgacc gtaagcagtt gcaggccgct attaaattag
     1081 gttttaaaga atggaccttc gataactaga tcttgagtca gggattttca ccgctactta
     1141 agatctgatg ttacataata taacttatca gagcaaaaaa ctgaataaat tggctggaat
     1201 aagaaatagg ccaaattgca atcctttaag caaaccgacg tcagaaaata ctcgaccgtc
     1261 tgcgggcaag ccgagccgat tgatttcaat agactgtttc aatcgagctc gaaaattatc
     1321 ctggatggct ttgccgagga ctttgttatg gcgacagacc acggccattt ccacattgat
     1381 atcctttgat cttgggtccg cattgtaggt gcctactaag atcgtagaac catccatcac
     1441 cgcccgcttc gaatgcgttc cccaccgcgt gtgcagagct tccgctacgg gagtgggata
     1501 attctgcgga ggatttcctc gatataagtg caacctaatt cccaaattac gccaaataga
     1561 catataggga taaagggcgg cgctcgtgta cgtcgcatcc gttgaattca aactatttgt
     1621 caacagctgt aatttaacct tctggccaat gttcatcaca tcttttagaa aatccaaacc
     1681 gtcgtaggaa accacgaaat aaggagattc tatcgcaacg gactcttgag ctgccaaaat
     1741 ttcctgaatg gcggcacgat acaccggact attgggcgat ttatctatcc catcggcaac
     1801 aaagctcgaa tctgtgcaga cacttgtaac ctgtgtcttt aacatctgtc ttccgctgcg
     1861 gttaattttt ttcagcaaaa tcgcatctgc tggtttggac gtgataaatt ctttggcctt
     1921 ttgaacggat ttagaatctg ctgaaggagt tttaggtgcg gccacggtaa gtccgctttc
     1981 ccagaaatct tcaaaggatt ttttcatcgc tcgcacgagc gttccttgaa catatagatc
     2041 ggtgtctaga aaattatagg tggggcttaa gtcaaaatac tcttcagcaa tattacgtcc
     2101 tcccgtcatt ccctgcccat cgacattctc cccgtcaata ataagagatt ttcgatgact
     2161 tcgaaattgc gctccaacca cgttgaagaa agacgtttta ttgtaatatc taatttcaag
     2221 cagtccctct ttaacagcgg attgaatgac cgccgcgtag tcttctttca attgaaaaac
     2281 cgcgatggag gagtccacca ataagcgaat cttgatatga tccttttgcg ctttttcaat
     2341 tagagcctgg gtaaataagc gtcccacttt gtccgtgttg tatataaagt attctatatc
     2401 tatggtcttt tttgcggtgg caatcatttc gaatcgcttt tgaagtgcgg ccacacccga
     2461 atcgatcaat tgcatttttt gcgggtctgc gaaggtgtac tgaagtctct cctgagaaaa
     2521 ggcttttaaa gaaacagtgc tcaacaaaat aaaaataaat aatttctgca taagcttaag
     2581 aatacgagcg acgtttattc cgtacaactc ttatgagttt aacaggacga gaccttggag
     2641 tagcatagca atgagagtcg ggctaaactg gactttgctt ttcctggttt catctgcaga
     2701 atgaatataa ttagaggaaa caataatatg gcaaaagcac ttggacttct ggctattctt
     2761 atttcaattt atggtttggc gaaggccggc gtaggcgata aggacagtga tctgtgcttc
     2821 gaagttaaag actcccaaaa atttttaaag gtgaatacga gggtggttcc tcaaggaact
     2881 cctcctgaaa tcgagaaaat tcaagaactc gagaagagtg ttcttaaatt ttatggatca
     2941 aaatgtgctg acgtgggtaa gtcatttcca ttagaaaagt tttttagcgc ggcctatgga
     3001 aattgctttg ataaatgcaa gaccattacc tataccgata aagatcttaa cgaaggaaaa
     3061 gctgcctccg gttacttcgg agatttttgc cgcagtcagt gcacagccta taattggcaa
     3121 gcatcagctt ggattgaagg aaaaaaagag ggcgaaaaga gttgcagtgc tggagtaaaa
     3181 acgaacatcc aatcacccgc ttctggtaaa cgctagcgta ctgctgaatt attttaaact
     3241 ttatattgct tagaactcgc tagaatcgtt tccgcgaaaa gcgctgtaaa catcgcaaaa
     3301 agagccattc ccgtcagcat taaaagaatc cccaggcatt ttccttgaaa ggttacggga
     3361 gtaatatccc cgtagccggt ggtggttgcg gttgcaaacc cccaccaaag ggcatctatg
     3421 aagccgtgaa ttctgggatt aatttcatgt tcaagaaaat aaaaaacaac acaaaaaata
     3481 ccgattaaga aattacctaa tagggttaaa agaagaaaaa caggacttct aataagaaga
     3541 ttcatctctg aaagaaaaaa cctcagactt aaaataaaat tcaactttgg ttttcttgca
     3601 tgcatagaac gagctcatct cctgcctata agttctaatc tttaagcttc ggggtgatct
     3661 taagttcttt tatttgatca cctcgtttaa ctttcattac tgtttccaca ttcggcttca
     3721 ccgcttgcag ggtataaaca taatcataaa tattttcgat ttttatacca tcaaattcca
     3781 caatcacatc gttttcttga attcccgctt gagccgctgg actctctttg gaggctccgg
     3841 aaacgcgtac acccttcacc ccttcttggg tataatctgg aatggtgccg aggtaagtcc
     3901 ggaagctgcg gccctccagt tgtttgttag ggttgcctcc cactttcacg tacttcaata
     3961 cctttaccga actatcagcc agaagccggg tgtagtccgc aacggtttta atcacccgct
     4021 caagccccgg ataatttaaa gtttccgccc ggtctcgagg ggagtggtaa ttctcgtgag
     4081 agcccgtaaa gaagttaatc gcgggcacct ccgccatata aagagcggtg gagtctgtgg
     4141 gcagataagg atcctcttga agagttaaag gcacgccttg gcgaatggag atctcttctg
     4201 caagctggga ccagtgatct gcagagccca caccttgaac ataaagtctt tcttgaaggt
     4261 gaccaatcat atccatatta agattggcat taaaatagtt tgaaattttt tgtttatggg
     4321 atttgcccca tcctttaaca aaagccgtgg aacccaaaag tccagactct tctcctgacc
     4381 agattgcgaa atagagattc tttttcaaac tgcccggttt ccttttgtat tgatcaccaa
     4441 agtagtgagc cagttctaag atccctgcaa ctcctgaagc attgtcatcc gcgccaaagt
     4501 ggatttgtcc ccgctcttcc gctttggcta aggaattgcc gatttctccg cggcccaaat
     4561 ggtctccgtg agccgagagc agaagaccag tgacagcccc tttcactgga agctttgcca
     4621 cgacgctgat cccttgggat ctctcgaaat ccaaagacac tgttgcggtt aaatagatca
     4681 tcggaatgga gatcgcctct aaagcctccc cttgattgag ctttcctaga agagagttga
     4741 agtcttgccc cgcagacttc aaaagttcct ttaaaggacc ctgttgaatt tttaaaaccg
     4801 ctagactgga atccgaaaga gtcccttcaa attttaactt gctgagatct tctttttctt
     4861 gggtggggtc cacaaaaata actcccacgg ctccttcatt tctagcgata gtgatcttat
     4921 gttgaagacg agcataggta tttaagtgat gccgcagccc tggagctaca tcttgaggaa
     4981 tgtctttaaa gatcaccacc cacttacgct ggaccttggc gtttttataa gaatcataag
     5041 ctggaaattt atccgtggcg ggagctttga tcccgtagcc gacaaaaaca accggagact
     5101 ccgcgatccg accgattttt gaaaaagata aaacgctgta atctttttca atttgcagat
     5161 tttttttaaa gtttcccacg aactccattt tattttcagt ccctagtttt accccggaag
     5221 taaactcaaa cttctcaaaa aaagaaccgt cattagcccc ccctttaagc ccaaaagact
     5281 taaaaatatc cgcaagtttg gaggtgtata ttttctcttc ggttcccccc actttgcggc
     5341 caccgagttt ttccgaggcc atataattca caatactttg cgcatcggac gcagtgattt
     5401 ggggtcttaa atccgtgctc gacagatctt tctctggcaa tcccaaaagc tttctcgcct
     5461 cagcatcgtc ccagtcagca atataaatct ggctttcacc tttttcatta cgatgagacc
     5521 aggtcatttg ttttccatcc agggaaaaac tcgctagacc atcaaaaccc tcaccgaaag
     5581 tcacgcgcac gggaggtttg gttccttcac tatccacgat aaaaagctca aagtttgcat
     5641 aaccaagaac actggaagca aaaataatgt aatctcccga gggatgataa tagggagccc
     5701 aggacattga ttttaaatga gtgatttgct tttgctccga accatccaca ttcatcgtat
     5761 aaatttcagc cgtggcccca ttggaagaaa agcgacgcca agtgattttc ttgccatcag
     5821 cactgaagaa gggaccccca tcatagcctt tggcgtccgt caggcgcttt acctgggtcc
     5881 cattctcttt catgaggtaa atatccatca tataggaagg atcttgctgg aacagctttt
     5941 gatcttcgcc gctgattttt tcagtataac ccgcacgatt tgaggcgaaa gcaatccatt
     6001 ttccatccgg ggagtaagaa ccctctgcat catagcctaa ggacttggtc aattgtttaa
     6061 gattttttcc cttaagatca gacgtgaaaa tatcgtagtt ctcgtcaaag ctccaagcat
     6121 aacgaccttt cactggcttc ttgcggcttt caatctcttc ctcttgtttc ttcttccatt
     6181 gaggatccaa ttgcgtagaa gaaaagagca attttttaag gctcggatga atccagccac
     6241 aggtggtctt gcctttcccg ctggaaacca aatcagtctt tccagtttcc aagtccatga
     6301 cgtacatttg atagaacgga ttgccaggat atctttcact ttgaaaaatc atttttttac
     6361 cgtcagcact aaagtaacct tctccagatt taggaccttc gaaggttatt tgatgagcgg
     6421 atttcaccaa aatgttcggc cgatcaacaa aaatattttc ttgcaccggg gcctcggcga
     6481 ggggcgcgcc ctgtgcatga ggattaagca ttaaaaagaa actcagcgag aagattttaa
     6541 gggtataatt cagattataa ttcatagttg cttgaggcta tttcaggggg tagcatgggg
     6601 tcaagaatga aggagcttcc cttgaaaaga attctttttg gtccattcat tatttgtctc
     6661 ttaatgctta ctgcctgtgc acttcaaaaa cctcaaaccg caagtccagc agccccaaca
     6721 acaagggata ggaatttttt taaatcccca gtgaccactg agaatgagaa agttgaattc
     6781 attgcccata ctgagaataa gcccatgaaa gcctatcttt cgatgaatct tccctatcct
     6841 ccctatcaag tcctttttga acagatccaa aaggcagaag ggatcgcact taccaatcgt
     6901 ggtgaagcgc acatgactgt catcaccccg gttgagtatg acaaggtttt gaaaaagcat
     6961 cttaacattg ctgaaattca caaaattgcc gaggctacaa aaattcagga acttcctttt
     7021 actcctgtat gtattggcaa aggacaaaaa gagttaaagg gcaaattaga aaaagtttat
     7081 tttgtcgtgg tggaaagccc tgctttaatt gaactgcggg ggcaaattga agaggcctat
     7141 attaaaaagg gggggaaacc tcaggaattc gttccggaaa gattcttccc acacgtgacc
     7201 ttaggctatt ccgctcgcga tttgcattat gaagacggcg ttctcaaaaa taataagagc
     7261 tgtctctatc aatttcagca ataacttagt aaatataagc tataagtatt aagatgcaga
     7321 tagattgtaa gtacaaaaaa gaatgctctg gttgccaata tttagatctg tcccttcagg
     7381 aacagcagca tctcaaaact caagcgcttt ccggacttct cagggatttt aaaatccctt
     7441 ttaaaagcgc cataaacctg accagcctgg gaccggcctg gctcagggac cgtgtcgatc
     7501 tggtttttaa ttccgtgggc acagagggcg ttttagggct ttacaaaaag aacagccgag
     7561 agatcctgga tattgaacaa tgtcagcagt tatcgccgca actgcaagct tggctctcag
     7621 agattcgcaa gattcactgg cctatccgca aaggttcttt acgtctgcgt gtcggccctc
     7681 aacgccaaaa aggaatctgg ctggattttg ccaatattga tataaaaaat cttctggagg
     7741 aaaaaaccat tctctcgaat ttgttaaact gtgcggtggt ggagattggc caaaaacaca
     7801 aagtgcttca attcgtcaac gggcgattga aactcaaaga cgccgagcct caggtctggt
     7861 ttcaaagctt tattgagacc caagcggtgg atcttttctg ctccatcgga aatttcaccc
     7921 aaacaggaat tcaagccaat cgtgacctca ccaaagttat tagccaatgg ctcgaggaaa
     7981 tccattctca gcatattttg gaatttggct ctggaattgg aaatctgagt tttcccgctt
     8041 tgggttacgc gagaaaactc acagcctgtg aaatcgatcg cggagctctg gcaggttttg
     8101 aaaaaaccct gcagaagctt tctcaggttc caggttttga aaaaattcgt gaacgcgtcg
     8161 aaatacagca aggcgatttt caaaatcgca atccccaaaa atttgctgaa tatgatacgg
     8221 ttctagtgaa tccaccccgc tcaggattaa aagaattttt aaacccttta atcactgaaa
     8281 cgaagaaacc gaaatacttt ttgtacatgt cctgctttcc agaaagtttt gtcctggatg
     8341 gacagcgcct tcagcaagcc ggttacagtt tgcaaaaact gaatatcgtc gatcaatttc
     8401 cccaaaccac gcactatgaa atcctgagtt tgtggggtct caatgaagag aaaaaagttt
     8461 caggccacaa ataattcccg gaatgtctcg gcccaaaata agagaaagaa gaggcaagac
     8521 caccacacca gtggccgcaa tcagagtcgc tcgaaagaca actcgcaaga aataccaggg
     8581 tgacaatcgt tctcgaggct cgccgagacg aatatcgcag gccgcttcgc caattgaagc
     8641 tccaataaaa aatctggtag tgatcatata cagccaagcg gcccccagat aaatcaacgc
     8701 gaagtctttc agcaatccct ggtcgatggg aactttgata atcttaataa aggccattaa
     8761 aaacacgcaa ttcatggcga tgagcaaaag cgcatcaatc gttgaagcca tcaaagacca
     8821 cagagtgagt gtataaccct tgcgtttccc gatgggccca ctctgaaagc ccgtgtccat
     8881 ttcccactgt aaattttgac gatttctaaa aagcttcttc aatgactcag gatactccga
     8941 tggcactggc actttgtctt cgggaatttc ccaggggaga tttggatttt caggttttac
     9001 tttttcaatg cgcataaaaa gatccctatc taactccacc cgaggtggag ttagagtttc
     9061 cgtttgtgga ctccacaaac ggagtcactg ctggcggcgg aatcggatcc gtcggtggcg
     9121 gctgaatcgg aggtggaccc ggtggcgttg ctggggtggt cggtgtgccc ggcggcaaac
     9181 agatcaacgg agctggtgga catgggtcga ccgatggttg cggattatga tcatcatcat
     9241 catcatcttt aaagatgaag tagagcgcag tccccaccgc cgcgaagata gcgccgccaa
     9301 tcaaccattt acaccaagtg caggcaccgg ccgcaacgca gtttccttgg tcatctcgtt
     9361 cttcatcttt attgcattct tttttagtat catcggcggg aattgcctca gcaggaacac
     9421 acgcttgtcg ttccacattg tcttcggtga ttgtctgtac aactgaaccc ggcgcgcaca
     9481 cacagacgcc atcgaccaaa gttgaaccag gtttttccag ggagcaatct ttttccggag
     9541 tcacaatggg gggtagcgga gtctcagggt aagttaagac ttccatttta cgtgtttcca
     9601 gagctcgaca ggcggactcc tgctcatcgc gaattttacg aatatcaccc aatgggggtc
     9661 ttgaacaaaa ctcaagcgct ctgtcaatat aggtattgag tttatccagg tgggccttta
     9721 taagatcata ttgggcttga gaaagcttgc cgtccttatc gaaaagcaaa ttaatatttt
     9781 tctccgcatc cgtcgttcca ttggctttta aataagactc gatgattctt tttttatcat
     9841 tggcttcatc tggagtccct ttacgaagag gcgattggtt tggacaactc tgagaagttg
     9901 aagtttggat ttcctgattc ttaccatgct ttacgcaaat tgcagctcca ttctctcgat
     9961 aaccataaac cagaggatta caggaaaccg acccattcat gcattgtcct ccaccgtagg
    10021 tattcatttc ttgaattaaa gcttcgcgac ctgggcccga tccaccgcaa gaagagtcat
    10081 ttccatattt cgcgatataa ccactcacta tgcatgggtc tcctgagacc gctccacgaa
    10141 tggatttaga ggctttgcca cccttcgctt ccgcgctccc tcccaaaagc aaccattgca
    10201 caaaaaaatc atgcctcatt tcattggcgg cttgagaacc ctgatatttt tccataactt
    10261 gttgagcatc ttccatgaca taacgaagac gcactagata ctccgctcgt tgtctcggct
    10321 tcaagcgctt aaactctcca taactcaaag ccacggactg attaagacga gttttgcgtg
    10381 gggccttgag aaccgctgcc tttattccct tatcccctaa gatgagctta cgaatcgctt
    10441 gctcatggaa cttcatatcg tctttggtca aaaacacatt attcactttt aagaacttcg
    10501 cattgttgtc gggattgtaa gtggcggtca ctgtttgccc gtctttagtg atcaaaaggc
    10561 gaactcgctc tttgccatcg ctatctttga tcgtgctcgc ttccacctca ggcatgaact
    10621 cattgcggtt catctgaacc cattgatcca tttctccgcg caaagtttta ggatagaagg
    10681 gtcgcatttt tgagtaaaat tcgccgaccg tcagctttct tttattcaga ccgctgtatt
    10741 tgagaaattc ttgaatggat tttttatcaa tattgtaatt ataatcggaa gccttggctt
    10801 gcaacttcgc cttcacgttc gctgtggcta ctgccttggc ctgcgcccct tgagccagcg
    10861 gcgaggccac catagtgaat acggcaacat tcacggcagt catttttatc aacgaaagtg
    10921 caactgtcat ccatctcata ccagaacctc ttttttctat acacttcttg tatcggattc
    10981 gaacgattaa aaatttaggg ttctagagat aggtcctagt ccgattctaa aatttatatt
    11041 tttttagact taagccaagc ttctaaatag ccacgacgtc cccaaccaaa attcttatac
    11101 ttcaacctta gaacatctcc gttgaactcg accacttccg ccttaaccac cttaaaatcc
    11161 ggcagttcgg acaggctaaa ctccacctca tttttgcctt ccaaggattc cgcttttttc
    11221 gccagacgga tacgaatacc agctgtagag acagattccg tcaaacccgc aagatctcct
    11281 cccgtatgca caatcacggg agttcttacg tcatatctgt gggccgttgg aaacatccat
    11341 tgctgacgac gatctaaata agggtaacgg gcgtaaaagg cgataatcaa aacagaaaaa
    11401 gtgaccaaaa ttgagaggag aatttgcact cgaacaaact ccggattcaa aacagtttca
    11461 tcaatggtga aggcgcgata agtattcatt cctaaaacaa tcaacagaga caccaccgcc
    11521 aatagccatg tcattttgtg cttcagcaac agcgcaaagc ctgagataaa tatgcccgca
    11581 agccaaagcc aatcaatcac gggaatggtt ttaagaagct ctacgaactc ttgtgggtaa
    11641 taccaattaa gaaccccgct tccagcaaag cttacaagga tgtttcccaa gggagccgcc
    11701 ataaagagca cagccaaaat atagattccc acgggctgtt ttaaatccga tgatggttct
    11761 tgagacatat tccccccaaa cacatgataa ttgagtcagc aggcaaaaga aagcccctcc
    11821 tagactatgg tctatcgccg ggaggggctc tttttaaatt tgaattaaca aatcattatt
    11881 tcaactgagg acagctttga atacaggact cttgtgacaa tttgatacgc gcccccaggc
    11941 attccggcgt ctgtctatgg cgaatcaccg aaagagtgcc taaattcgga gtatagttgc
    12001 ggttattcaa tcgagccaga caggcatagt aggcatattg cgtaagccct gtacgcatct
    12061 tatcactctc ataaagataa tccttggctt gataacgatt catgcctacg gcatttttac
    12121 tcggactact ttgaataacc acaaaatcaa agtctttggg ttctaacctt gaacaagcgt
    12181 cagcgttttt cactccatta ataccgaaag gatcactccc cacggaatca atcataatca
    12241 catgcccatg aacagccacg atatcccctg ccttcagatc tgacttcgga gtcaccgtga
    12301 tcttagcaag acaatttaaa ccatttctcg ggggatccat atacatcgtg gcactgatcc
    12361 cgttcacccc cgtggctttg acgactttat ttgggctcaa gcgaagacca gccgccgcta
    12421 aacctgaata cacaaatccc gagcaatccg tgcccaatac tttagtgcca ttgcctccat
    12481 ccctaaaaaa gttcagaacg gagtttgcac ttgtggtcgc gtaaggtttt ccaccataat
    12541 cgtagatcat cggataggta ctggctttta aacaaccatt tgcatagcta ttgatatttt
    12601 tatagtaagg gtgagaatgc aagagggccg tgacattact gatctcacgc cgattgccta
    12661 ctccattggg atgttttccc gtgatcgtga tggctctgtc atcgatatcc tgagtgttct
    12721 tcgtcataat aggctcatcc agaacctgac aagattgata ggctgtcgcg aatgtaaatc
    12781 ttattccgaa aaccggacga ggcaatgaag tcttaacaaa ctcaggggtc gccgcccctt
    12841 ctccttcctc tggcactgga gccacagggg tcgcacattg aacatccatg gcggcaatct
    12901 gttttttgat tttcgcgaat tgagcttcaa ttttattttg caatctaact ttagcatccg
    12961 ttgtatgatc cccaagctca agggctgaca aaacctctaa cagaccttga gcgtcttgga
    13021 cctcctccac ttgaggaact tcattcaaaa gaatattata gagctcattc atatcttgcg
    13081 cgagagcctc tacctttctt tcattcaaat tccccgacgg agctttcacc gtcttcaaag
    13141 aagacctcaa gggatttttt aattcttcca gcgaaagcat tgatttctgt tctatcagat
    13201 aaccattgat cgcatcccaa gttttgcttt caaaatcgtt acaaccaata gccttggttg
    13261 tatctgtagc cacttgttgg acggcttcaa agttttttgg agcacaagcc gaaagatata
    13321 aaacacctaa aactaatact gtggataaat tggatttatt cttcatgcct ttattttcgg
    13381 cacttttaaa aaatctgcta atccagaact gaaaaactag ccctgggtcg acttgactaa
    13441 ggcctgtcaa taagccttta cttatgcgca tgaatgaaaa atttataaaa actcaggttc
    13501 tgtacaatct tatcgactgg gaaccattta ttttgttggc ctgtttactg gccattactt
    13561 ggattttcta taagtttttc ttgcaagacg cttcgcctga aaggcacatc agcattcgct
    13621 ctcagttcca agctctgatt cgtcattttt ttatttttac gatttcgttt gtcgctttca
    13681 tctctcttca tagcattacc ggcggcgact ttgtaaattt cgcacgcatc actccttacc
    13741 ttgctttgac gactttcgca tggggctcca ttattttcgt aaaaacctgc cgactgcttg
    13801 tccttcaata tttatttcta ggatcgatgc gtgcgggcgt tcctgtgctt ttagtaaata
    13861 ttttttcttt attactttcc atcattctga ccgtctgggc cttatctcag attttcggtt
    13921 tagatttagg acctttaatg gccacttcgg ccgtcttttc catcgtcctt ggacttgccc
    13981 ttcaggatac tttaggaaat ctctttgccg ggatttcttt gcagattgat aaatcctatg
    14041 aaattggcga ttggcttgaa gtcgtccaag gcattcaaag agccacgggc caggtcaaag
    14101 agatcacctg gagatcgaca aatctagtgg gcttgtctga cgaagaaatt accatctcaa
    14161 atcgcgctat ggccaacgct caaatctcta atttttcgcc ccagggccac cccatcgttc
    14221 gcagccaagt cttccgccta ccttatggct gtaatcttga gaaagccaaa gaactcttag
    14281 agcgagccgc tgtagaaatc tcagatattc gcggattgcc ggctcctttt gccttcgttc
    14341 aggaaaccac cgaaaattgg gtgggaatga aaatgatcta ttttatagat aattttggca
    14401 gccaatactc tattggcgat aaagtattaa gaaaagggat tgatgccttg aggactcatg
    14461 gtatagaact cgctcgtaac gtgatcgaag ttcaccaaaa gagtatagat aatgagcaaa
    14521 cccgttaatt taaatacaca tttaaatatt gatctgaatg ctgacctaaa aaccgatttt
    14581 gaaagcccct tggccaccaa aattcgtaaa cagatcgttc aagctttaaa tgactttgat
    14641 ctgatccatg acggcgacaa aatcgctgtg gctgtctctg gaggcaagga ctccagtatt
    14701 ctcgtggcgt tattgaacga aatccgaaag cgctctgaaa gaaagtttac gattgaagct
    14761 ctcatccttg atcaaaagca acctggcttt gaaattcgcg ctttcaaaga atggattgag
    14821 tctttgggaa taaaactcca tattcttgaa aaagacactt acagcattgt gaaagaaaaa
    14881 actccggaag gctccgtcta ttgcactttg tgcgcaaaat tccgccgagc catcctctat
    14941 gatttcaccc atgcccatgg ttttacaaaa atggctctgg gccatcaccg tgacgatctt
    15001 atcgagacca ctctgttgaa catgttttac attggccaaa tgggcacgat gtcgccaaag
    15061 ctaaagtctg atgatggacg caatattgtg attcgacctc tggcttatgt
//
LOCUS       DATBTE010000370        47762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_118694, whole genome shotgun sequence.
ACCESSION   DATBTE010000370 DATBTE010000000
VERSION     DATBTE010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 47762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47762
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118694"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VIG33_13870"
     CDS             <1..730
                     /locus_tag="VIG33_13870"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEY8271973.1"
                     /translation="NWLLKELAKINRASPKNVKKPIFITGHSLGGALANLIMYSLLEH
                     NDSTKNQNSRLNLKSLYTFGAPRVGDQIWAKRFHQLAEQMDVGLYRMVNFSDPVPSLP
                     CLNYSHAGSIVFMDLNSNGFSKVIDSVKVILNPLNRVLNLGTTHINLTNCAYETVIYY
                     PRHIKEMINNHFMTSYYQSLSFLKNSLNEKMETQRKQALFNHDYKTAEQFTYPSNCQK
                     PNVSNLNLEGLELNYQLLKNNLED"
     gene            complement(749..2332)
                     /locus_tag="VIG33_13875"
     CDS             complement(749..2332)
                     /locus_tag="VIG33_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271974.1"
                     /translation="MFIKLAGLITFAVLISNQNALASTFLYNGTYYSTQDHSEVLETL
                     KKYGVQKAGNLQLGAVYQGGPYFHFYVSQENWQSLYRELMQKGDLAIEKSLSEKVSSP
                     GTLRVILRMTRPKEKKDILSLHQSRRNPSKEIAELQRIFPEGKVTGINSFIIKGDDEN
                     FQKIFEILPNEGPFILEKNPHWDQSRFEVAFSKRKAPSIAPVETEGSSADYVILWTAE
                     TEDSKALESQWRRQFQFEITPQGYLFHIEPKTFRGWAQDFLKKGSLTVHKTRNTAKDS
                     EQISVLLINENIFEKPLDETPAKTPEEVPVTTPVEPVFTYNDLNVWMGFFLTKSSPQI
                     IVADDGTESFMPQKIELKTFKNIEFLRGWNEIQADLRYFNFDKSLVLPETTIWDAGIG
                     REFFSKDFKDRLGLVASGRIKNVKQDDISVVLMGIGLRYEGAFPESLNAFFKKIPFLS
                     YEKHFLLRGDFFPGTLDSQVTIQNSYFVQFLIKMFFTPQLYGHMHVFTTSENLEDLGS
                     QFRFHYGTTNFGFGAGYSF"
     gene            complement(2453..4432)
                     /locus_tag="VIG33_13880"
     CDS             complement(2453..4432)
                     /locus_tag="VIG33_13880"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8271975.1"
                     /translation="MKPQARVMVFSVFTVLVSSLAITACISSPVSVPSQIYSQPSSRA
                     PASLGRGNYRRHRRQNTVLIKLRENPTPEQLAALKALYEEFDLRAEKRLLKGKVVRLR
                     IHNNSYSDQEEDIVEQIKKTEAVQFSEPDYIVPHAFVPNDPFYANQWHLAKIQSELSW
                     NYTQGNSAVVVAVCDAGFDLTHPDLASQFILPGYNTVKNNTEVTNLHPHGTMTSGVIG
                     AMGNNSLGVAGMAWRIRLLPIQISEQGDGVSTYSDVAECLQYASDQGAKVVNLSYDYA
                     YASEVVKDAALYLRNAGGLLVAAAGNSSSDISSWGASPNMIIVGATDSADALASFSNH
                     GTPVDLVAPGENIYTTIPGGYEVVSGTSFSTPIVAGTAALLYSLKPNFTPDQVENFLL
                     STVQDLGATGYDLTYAGGRISAGLAVQKAAAAVLTDNVAPSVAIAFPANNAIVSGILN
                     IQVSASDNLGVAAVTFYIDANLLGTSTAVPYTFGLNTITLTNGAHSLSVKAVDASGNS
                     SMSTITLNVNNQTAVAPAILLDNLTPGISDSSHSFIGKWCSSNLSGSYGITSLISCGA
                     GTDIYRFKPSISAKGNYQISVHYLSSKNFSGKVPVTIKSATGSVTKRLNMRSGGGGWT
                     SVGTYSFSAGNLNFVEFKDTAGIANVDGVKFEPVP"
     gene            4633..4875
                     /locus_tag="VIG33_13885"
     CDS             4633..4875
                     /locus_tag="VIG33_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271976.1"
                     /translation="MGVCETCGASYERSFKIIINNETHEFDSLECAIQGIAPTCPHCG
                     CRVIGHGVDSDGVTYCCHHCLWIGKTKSFDEESPPI"
     gene            4926..13697
                     /locus_tag="VIG33_13890"
     CDS             4926..13697
                     /locus_tag="VIG33_13890"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY8271977.1"
                     /translation="MSSFFKQLNKTDDDFRVFQEPIRSEIYSATRLESQAEILAHSHL
                     VSKTPRKGHRLGPRISANKRLLEKSYLNLIHAVDEHRAITPAAEWLIDNFHIVRAQLK
                     DIRDLMPREYYQELPKLLIGPLRDYPRVYGIAWYYVAHTDSHFDADSLKRFLFAYQKV
                     QPLTIGELWALPITLRVVLIENLRRLAARIVGSQEARQQADQIADEVLGLGSQPPRLI
                     VEILAELERIPFSIWLAVQLLQRLRFQDQKVEPLLKWLDQRLEKKHLTSDTAVTIEHG
                     RQTAANATVRNIITSARLIAAFNWQDFFEEVSLVDQILRQNPFYSLMDFATRDRYRHS
                     LEHLARHSPYSQIEIAHKLILLCEKGKKPGDLRSSELGYYLISKGRKSLEKEIKFRLT
                     WMENLNRGYFESNTEIYLGGILFVSLLLLSLPILATRNLDLDRWQVYIFVALGILVAS
                     EVGVAIINRVTVALLGPKHLPRIDLEKGIPESSKTFVVVPTFLNQAEAIDSQLEQLEI
                     HYLSNPEGHLHFALLTDWVDATTEYLQQDRPLLNLAAEKLKILNSRYPPAPDGHPRFH
                     IFHRKRLYNPQEKKWIAWERKRGKLHEFNRLLLGAQDTSYIPLEMNGPIEVPSEVRYV
                     ITLDADTKMPKGCVAQLVGTMAHPLNEAFYDDDKGRVTEGYGILQPRITPTLPSTQTG
                     TLFQFLSASTAGVDPYASAVSDVYQDLFNEGSYTGKGIYNLRMFERALEKRIPENSLL
                     SHDLFEGNYARCGFLSDVEFFEDFPGHCLAASLRTHRWMRGDWQLLPWILGRGGASLS
                     TIGRWKMADNLRRSLFQPAAFFLLIFALATSLQLAWIWGLFTLLALANPPLITFVTDL
                     FNKKRSVRVLEHLKFALTDFQVGLLRTLFFFLLLPYYAWLSGDAILRTLFRLFISKKY
                     LLEWIPAAQAEAATSLNIKSFFYEMRGGLILTAFALPAVVLFNPPGLFLGIFLFLPWL
                     LSPWYAYWISRPFEKKTLRPMTSSDQEILRLTGRRIWLFFASFVKSEDHFLPPDNFQE
                     EPHPVIAHRSSPTNFGLYLLSVFSARSFGWITLHDAIHHLSKTLESLHELPKYNGHFY
                     NWYETTTLRPLDPKYISSVDNGNLAGHLIAVAQGFNHILSQKIDMANLTVGVYESLEL
                     LKQALSSKKSPLLTSVHQIEKALSLSRDEPMDDAKMKNLKNQSATILQQAAHSSNGSG
                     DPETFNKTLGETLTWAQALHQDIVSLNVEYHSLLSWFDFIKKSLPGQNPPEILSAWKK
                     IQLKLSAEISLEMIPDHCAVISEKIIDFEKQIIVQNSETGTNAETRLFLGNLLDRLDT
                     SMHAAKTLIAKAKCSQAIAYQLFNEMDFRLLYDKNRKLFSIGYRVFEKILDPSYYDLL
                     ASEARLLSFVAIAKGDVPVSHWFSLGRSLAQVGDKKGSVLGAALVSWSGSMFEYLMPS
                     LVMKTPEGGLIKNTCELVIHRQIAYGKERSVPWGISESAYNKRDLHLTYQYSNFGIPD
                     LALKRGLGTNIVVAPYATLLAAMYEPSLAAENLRLLKTIGACGTYGYYEALDYTRSRV
                     PTHRNFVVIRAYMAHHQGMSLVSLANVFYNELLCRLFHNEPLVQATEILLQERTPRAV
                     GVLPTSEDHAPLFIREDTGHLSRRYHSVNLPTPRTQILSNGNYTVILTSAGSGYSQYQ
                     NLAVTRWREDVTQDPWGSYIFIKDLERQKIWSPTYQPLGTPPEDYEVTFAEDRVLFHR
                     EEEEIESELEIFVSPENNAEIRQLTLTNKSEYFRNIELTSYAEIVLNTPAADMAHPAF
                     SNLFLQTEYLAATQTLLVRRRPRSSEDPTLWAAHVIVATGEITYEIEYETDRAQFLGR
                     GRTIHYPQALFKNQKLSKTVGAVLDPIFSLRTRLRLEPGTATRVFFSTLVAESRKEIL
                     RLAESFQELSTYERVSSLAWSQAQVKLHYLNMEPDEAHLFQRLATRLLYLDSSLRPSN
                     AVLKRNTQNITHLWAHGISGDFPVIVVRIEDAEDRGIVRQLLKAQEYLALKRFVVDLV
                     ILNAKSSSYSQELQETLESMVHSRLSAPLPLPYSRGKVFILREEPLPREDRLVIYAEA
                     RITLSAGHGSLSDQINRMLQPSVPPFTGRVYRDARAAVFLPPPPLEFFNGLGGFTPDG
                     REYVIVLNNSSQNTPAPWINVISNGTFGFQVSESGSGFTWSLNSRENQITSWSNDPVC
                     DPSSEAFYIYDLDSKCLWTPTALPIRLEEASYIAAHGQGYSRFEHLSNDIHSTLTQFV
                     PFGEKLDSVKVSQLRLENRSEQWRRLSLYAYVEWTLGFLRSTTAPYILTEIDEASGAL
                     FATNVRNSEYGHRVSFAIFPNRKQQVTGDRREFIGRNRNLAAPLGILRDQPLSNHVGA
                     ALDPCGALQIDVQIGPGEVLEVPFFLGQAANREEARSVIFELSSRSCGDLLKKVSQQW
                     DEWLEQIQIETPDKAFNFLLNRWLPYQNLSCRYWARSAFYQAGGAFGFRDQLQDMMAT
                     VFMAPQMVRAHLLRAASRQFIEGDVQHWWFPSSDRGVRTHFSDDLLWLPFATFHYVEV
                     TGDLSVLDEIISFLEAPLLKPEQEDAYDVPVVSAQTGSLYEHCVRALERSMALGAHGL
                     PLMGGGDWNDGMNRVGKNGKGESVWLGWFLYLNLQQFSHLAQTRNESARAEIWLRHAD
                     QLKAALEREAWDGDWYRRAFYDDGTPLGTAGDSECRIDSLAQTWAVISGAADLARARH
                     AMQALDNILVRRPEKLVLLFHPPFDKTPLDPGYIKGYVPGVRENGAQYTHAAAWCVIA
                     WTLLGDGERALELFNLLNPINHALSPDEVRRYKIEPYVLAGDVYSQEPHIGRGGWSWY
                     TGSAGWMYRAALEYILGFRLKGQELFLTPQISPQWRFVKLRYKYRRSVYAIHFVNPEV
                     YTRKGLQITLDGTPLASGAGIPLKDDEKIHQVVVTLQ"
     gene            complement(13684..14193)
                     /locus_tag="VIG33_13895"
     CDS             complement(13684..14193)
                     /locus_tag="VIG33_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271978.1"
                     /translation="MNKKFFALMTSSLLTAFLLGACSSEPKKTELERQVKAQPEANSP
                     EQIAQRAAEAFSGAPGLSAEQKQKLMAIYAKTYAEAMSIRAEIGQSKSLLFKLIASTN
                     YKSTDVNQLKKKITALDQKRLAVMFQALEDVQKVVGYGADKEEIYKHLRDFEFPQQHG
                     RESAQITVR"
     gene            14354..15244
                     /locus_tag="VIG33_13900"
     CDS             14354..15244
                     /locus_tag="VIG33_13900"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEY8271979.1"
                     /translation="MPKLNHFELVLAAIDFIENNLKEEISIQEVCKEVPLSSWQFQRL
                     FRALVGNSIGSYLRGRRLTLAAHMLADSKRILDIAIDLQFGSQEAFTRAFKAQYRITP
                     GQIRNHPEYIKLYKKPRLTKDTLAHLQNGIQREPKICLLGPFNLLGKVTTITSFLGKN
                     PDFESKLPGFWKEFNREKGAHSLNNNTESYGVAISSSQDMFEEELKYFAGIPVPAERE
                     IPKEFVPLHMDEQLYAVFENKGLADKSQLTIDYIYGVWLPESGYQRARGFDFEIFDHR
                     YSLDSSDSLSQYCIPIEKIS"
     gene            15358..15804
                     /locus_tag="VIG33_13905"
     CDS             15358..15804
                     /locus_tag="VIG33_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271980.1"
                     /translation="MEPKVMNRRQVLGLSSASLLMLVFGAKAKAHQGDTAELTQDFLK
                     IESGPGQIVNNSFAHHHHYLEIPLQYIQEPPAAGLQIHTGWAIFRNKFANFAPHFHTV
                     PISQQELEAVGRGETVIVEDTVKDHKYVLTLSSSITSWHHPERHRS"
     gene            15870..19025
                     /locus_tag="VIG33_13910"
     CDS             15870..19025
                     /locus_tag="VIG33_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8271981.1"
                     /translation="MGKLKKNLKSTTLAYALSLSGLFITGCTASNTPENEFLKFGFME
                     GIYSTRPQTAEPVITTLKLKNPALLESAERKNGVLSVDKNLVDAIQQEQTATIAELQK
                     ISPEIKILIRYRLVLNALTILAPPEALDKIRTLPQVVMSEKSSTFARPLPLDTEKKGG
                     LVGSHTSVNFIGAEAAYAQNIHGEGMKVGIIDTGIDYTHKMFSGEGTEEAYKNNNPAQ
                     VNPAFPGSKVVGGIDLVGTEYDSASADFSQHIPVPDLNPLDESGHGTHVAGTVAGIGD
                     GVNTYSGVAPAASLYAIKVFGANGSTSDEIVIAALEYAADPTGDLSFKEQLDVVNLSL
                     GSGYGNPHTMYSSAVKNLTHGGTVVVASAGNSGNKSYVTGAPAVVNDTISVASSIDNM
                     DQNILFPTVQFAFANEILNAEAMEGAVTKPLSEISSLKGEVIAVGYADKDFDQALKDQ
                     INGKVALVDRGRVTFSEKIQRAQEAGAIAIIVVNNTEGEPISMGGEGKFNIPGVMITK
                     AAGTTIKSHLAVTVDLKSTLKVDKPWLVDTISDFSSRGPRSEDGMIKPEIAAPGSNII
                     SADRGGGEKGVSMSGTSMAGPHVTGVMTLLKQKYKNLNTQELKSVIMGHSKVIADPDK
                     KIYSVSRQGAGRVQIAESLNAKVVTVPAALSFGITDIEKQKTLSKNVTIKNMSTETLT
                     LKSEWSGSAGLVFSTNNLTLAPGESKDLTVTVKILAAQMKSANDELDGFLKLTSTQAS
                     GEETLVQIPALVVARQISQISAKSLLVHATSLADSAGSVAEISLQNTGLNKGRAYLFN
                     LLGTDARKKDAKPDPIHNHNCDMQSAGYRIIEKDGIRILQVAVKLYERLTTWNSCEVN
                     VQIDGNNDTQTDQEIAGVPMSSLPGLAGDSFVSLLLDGNKAQDLRKRFETNIANNIKD
                     AKEDYSEAILDQRTMQVVDGSTLAIIEANISQLALADTGELNIKVSTTHQDTGVIEYD
                     DFLGKQESQWQKISVNPLAQSFAQLPETVELKGQETQTLHLQKGYGNGDLILYAPQNR
                     SVVDVLLEDTQSQTVPASFEN"
     gene            complement(19022..20194)
                     /locus_tag="VIG33_13915"
     CDS             complement(19022..20194)
                     /locus_tag="VIG33_13915"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEY8271982.1"
                     /translation="MRALCWQGKHDVALKNVKDPSLLNMHDAIIKVTTAAICGSDLHL
                     YDGYIPTMEKGDILGHETMGEVVEVGSAVKKIKVGDKVVIPFDIACGSCQFCLEQEFS
                     ACDNTNPNSHMADKLYGYSGSALFGYSHMFGGYAGGQAEFLRVPFADTNSLIVPKDIS
                     DEKVLFLSDILPTGYMAAENCHIKPGDTVAIWGAGAVGQMAIRSAFLLGAERVLVIDH
                     YEGRLQMAAESGAEVIDFEDVEGILDEIKYRTGGRGPDACIDAVGMEAHGHTVTANLD
                     RVKAAVMLATDRPDALRQAIQACKKGGTVSVPGVYGGVLDKIPFGAAFAKGLTFKMGQ
                     THTQRYMQPLLDRIVAGELDPSFVITHRVRIDSGPDAYKTFSDKKSECIKVIIQMH"
     gene            complement(20209..20784)
                     /locus_tag="VIG33_13920"
     CDS             complement(20209..20784)
                     /locus_tag="VIG33_13920"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY8271983.1"
                     /translation="MDPFVSTNKDEVQPQPPLREKVEKKPDEELKSRHAVTIRKSPSE
                     VYTFFRDFKNFPLFIQNLIQVQVLSEHRSHWILQLKNEATVEWDIEIIQEIPDQLLAW
                     RSVEGSEVETAGTIRFEKAPANRGTVVRLAMDYSLPGGKLTELTLLFTGEPPELVIMK
                     NIKRLKAYLETGELPTIEGQPSSTSGSSLTH"
     gene            21039..22157
                     /locus_tag="VIG33_13925"
     CDS             21039..22157
                     /locus_tag="VIG33_13925"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEY8271984.1"
                     /translation="MRSVFDDLSQFRYLNTMNIQKTLTTSPKTLPPADKLGFGQFFTD
                     HMFVAKYREGQGWYEAKILPYSPLSMDPGASVFHYGQALFEGMKVFRQDDGKIVFFRP
                     EFNHQRLADGAKRVCLEAPPLELFMQALQELVAVDERWIPKEPNTSLYIRPTLIGTEA
                     FLGVRPSHETTFFIILSPVGSYYSEGTQPVKIWTEEKYLRAAPGGLGAVKAGANYASS
                     LKAAYESKQKGYAQVLWLDTDREGIEEVGTMNVFFVFDNEIVTPALNGSILAGGTREA
                     VIHILKSKHLPIVERRITITEVIEKIQNGKLREAFGTGTAAVISPMGILAYKGKEWVI
                     NDNKNGALSTELYKQITSIQHGTAPDIFNWIVPLQKNL"
     gene            22163..22735
                     /locus_tag="VIG33_13930"
     CDS             22163..22735
                     /locus_tag="VIG33_13930"
                     /inference="COORDINATES: protein motif:HMM:NF017747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEY8271985.1"
                     /translation="MKLYTKLYRKADYLSMPWKNGLGMTEQIQIFPENCLFSEGQDFL
                     WRISSAKIRGASPFSQFPGCDRWLMVVTGQGLLLDGGALLPQEPLHFSGEPPLHCALL
                     GGEVTDLGIIYRRDKISAMMCREEFMDTCSLDLPAGLHFIYCFDEPLKIAEQILESGD
                     SLEIAGPAKVTLDSSFGGKAKYVRIHIQEK"
     gene            22932..23372
                     /locus_tag="VIG33_13935"
     CDS             22932..23372
                     /locus_tag="VIG33_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271986.1"
                     /translation="MDPSMTVHRSDAHATQVDLWASRNIKNLSAEERPQLYAKAIQAI
                     ERRSLNTLSSVTVLVIVDRAIHETKENFPLLTPIKSEAEGIDFSALFDKPDMNYEELE
                     GALRALLIELLNVLGNITADVLTVPLHKELMDVTIVKKKNRERK"
     gene            23369..24772
                     /locus_tag="VIG33_13940"
     CDS             23369..24772
                     /locus_tag="VIG33_13940"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEY8271987.1"
                     /translation="MKDIRVATGVRNLDEILCGGLPNGTLTVFAGTPGSGKTILSQQI
                     AFKNASPKRRVLFFQTLSEPTAKTLRYLKQFSFFDQKKLDDGSVQFVDLGDILRTEGL
                     EKALSLLMTHVKRVKPFFVIVDSFKIFEDLAKSREELRKFSYEVAINLMGWECTTLLL
                     GEFSQKDIETNPLFSIIDGIVSLKHYAEAGEEQRLLQVIKMRGTDHSRDEHTFTITPS
                     GLEIYAPRVTIRRIPGSDIMSKNNGPARAKLRISNLDSLLGEGIPYGSSLLISGVAGT
                     GKTILSLEFIYRGAKDFGEKGIFFSFEETAERLITCARNMGWEIDDQIKKGNIEIVYI
                     PQTEIQVEKHLLMMKDRIELMKAKRVVIDSASVFVHKIKDPQAVREKIFQLGTLVQMA
                     QGIGLFVTDVHYGSNKISRFGVEETVVDGIILLTSSEVGLRRERFLEIYKLRNTSHSD
                     GRHKMLIEQGGVRLEKF"
     gene            24785..26215
                     /locus_tag="VIG33_13945"
     CDS             24785..26215
                     /locus_tag="VIG33_13945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY8271988.1"
                     /translation="MTDSPLNDEKSFSLTKKLPTVEHKPRAPDNTLSWQEQAYDRLKV
                     LYEVGKLLSTFENVEKTFPKILDLCSTSFPFRTAVLIEKRGSSVMTAMWNVETVTAEQ
                     IARATTSSKESFIYLTGASKCESANLRTTEMSSRTLSHAQTRTQSENKDPESYIVLPL
                     YVDQLPAFGVLQLGGSYPLCEKDVEFVDALSNLVAIAIDRYYRIQYERELKAKEALES
                     SHKLSRSEASVTDLETERELRENFVSLLSHDLRTPLSAAKISAQLIQREVGITETTRS
                     LASRIVFNMNRVDQMISDLLDANRIRSGEKLPLNIEPFDLIALVGRTLEELTTIHGER
                     FILKAPDILDGYWDQRSIRRIIENLCNNAIKYGSPDAPTILSLSQNTEKITDQITDQV
                     TIEVQNTGDLISPEDQKTLFQQFRRSKTAQDSGKKGWGIGLSLVRGVAEAHGGTVKVA
                     SDAERGTVFTVTLPIDSRTLISKEGP"
     gene            26212..26571
                     /locus_tag="VIG33_13950"
     CDS             26212..26571
                     /locus_tag="VIG33_13950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY8271989.1"
                     /translation="MKKTILAIDDNKETLFLEGVVLTQAGYQVHTALGGEAALKMIHE
                     LKEIDLILLDYEMAGMNGPEFLAALEKQYPLVFEQTPVIYATAHENPPQKLNKSWIPK
                     LRDIDKFIKDVERFITP"
     gene            complement(26568..27944)
                     /locus_tag="VIG33_13955"
     CDS             complement(26568..27944)
                     /locus_tag="VIG33_13955"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEY8271990.1"
                     /translation="MNRAHQRPKVRCFRHLFAIKLNKTSFRYGMNVANAMPLKAGSSM
                     YSFVILMLLVSSCQVVKADFILTMGGDINLAKDERDPESPVYSAPDVLARLPQQPRSV
                     RLAVQAQAVFKKLLTGDINFGNLETVVSSDRNLTDYDKKFTFMTHPEVVDILADYGFN
                     LFSTANNHVFDFGESGAADTLRNLAELKESIERKSMLNGRPGKSLYHAGLAGNIDEAT
                     EPLIIEIEGITIAFAAIGFGGVSTGSDEGSILRNNPGIVAMHNKALVNKLLQNMAHSN
                     ANLKILSIHNGTEGVFELDKGQRARYHDYLNRGDLDLIIGHHPHVVRPVENVNGKLIF
                     YSLGNYFMTGAASRDNESVRGNYGLFSRVYFEKRPDQNRINGFRLVTEAAELIPLTHM
                     HANLNIKSSAEAMANLSDLQQLSSEQLGNQALPFTLREQGVGVYCGPQVLGSRAQSLC
                     SAEGSWPH"
     gene            28024..28803
                     /locus_tag="VIG33_13960"
     CDS             28024..28803
                     /locus_tag="VIG33_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271991.1"
                     /translation="MISLWCVIGLIAATISVSTLGATFSVVGLAALFSGASLAVSAMA
                     ASLELAKFILAAYLHQRWTELNIIFKAYLVAAIIILSAITSLGIFGFLSNAYQSASAA
                     LEAESVKSESLKAQQAHNQAEISRLTRSIDEIPANRISKRIEVRKEIEPAIAALTKQT
                     QQLESLMTQSNLRTLELKNKVGPLIYIAKAFKLDIDTVVKYLILLLVSVFDPLAICLV
                     IATSGALETRRRGVAASPPPSGIEDEVIQMRFVDDKDQSTG"
     gene            28807..29508
                     /locus_tag="VIG33_13965"
     CDS             28807..29508
                     /locus_tag="VIG33_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271992.1"
                     /translation="MFLVKVLERKGVIRFFGFLLLLSPFFNTFAKMSLLTELSNRWSF
                     KVFWQILMMGSLFNQILYVATLIIGILMLRGSVKAWNFALVLLAGYIALQVSDFGHVR
                     ASKVTWLFFITNIFVFLFIADQLVWKVKATKPAPTKEKVPAPLIHKKIIIHFAGMGPW
                     AQVLSISNKGIHVRSLKAPPLDITSRELEISLKNGLTLRTRFASNSNQDYFFNYISLS
                     PEEINSLNQWILSIS"
     gene            complement(29512..29814)
                     /locus_tag="VIG33_13970"
     CDS             complement(29512..29814)
                     /locus_tag="VIG33_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY8271993.1"
                     /translation="MVQCALFMRLEAKPGKEGEVEEFLRSGRALVEEEQGTTAWFALR
                     LSQREFGIFDAFPDESGRRAHLSGKVAEALFAKDLDLLAKEPVIEKIDVIADKLPM"
     gene            30185..30475
                     /locus_tag="VIG33_13975"
     CDS             30185..30475
                     /locus_tag="VIG33_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271994.1"
                     /translation="MASKDASEVNSGPMYATNGVLSVILGGLILRSFPISAFWTIGVF
                     VGIDLLVSGASLIGLGVTAKKARKELVGQVYSTLHPEPGSRTELDREEHPLH"
     gene            30583..31191
                     /locus_tag="VIG33_13980"
     CDS             30583..31191
                     /locus_tag="VIG33_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271995.1"
                     /translation="MQKHIRLFIFVVLSLNFSFTAARAAVSPISVAILPPVQFPPGDF
                     SVTGIRASALWGKHRDLYGLDFGLLGNITEQSFTGLSASGIFNNTRGVTNILGLQFAG
                     ATNINTNKVSVYGLQATLGVNYNSAASTVTGVQLALLANVSAFTEIYGLQVGLYNRAK
                     DVYGFQIGLVNVADNLHGVQIGLVNFNHKGPFAISPILNIGF"
     gene            complement(31287..32936)
                     /locus_tag="VIG33_13985"
     CDS             complement(31287..32936)
                     /locus_tag="VIG33_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8271996.1"
                     /translation="MKKNLSLNVKLLMLCAFLLSISVIVGGVSYQSMSSVTGNYEFVV
                     DKSLPKTKLSYEMMLAYRRVRIMVRTLGLQGLSKADSEETIKGALNTIEEYEKFSKEY
                     VALGFIPGQKEVFDKLHSSWESFKATGEQVISLHKSGKPEDHEKMLQIFLKDCPEKAK
                     IFTAAAEELIQFHQKVLEAKVAEAKAAEKTGNQISLLVILAGLGLGLGIGFFIAMSIT
                     RAVGAVSKDLALGSDQVAQASNQIAGSSQSLSEAATEQASSLEETVATMEELTSMVGL
                     NTENGKQAASLAASTREIALKGEMEIKTLIDSIHTISADSKKIEEITNVIDDIAFQTN
                     LLALNAAVEAARAGEQGKGFAVVAEAVRNLAQRSASAAKEIAEMIKNSVEKIEVGSRQ
                     ANQGGAVLAEIVTAVKKVADLNNEIANASEEQRNGIVQIGKAMNQMDEVTQQNAAAAE
                     ETAAAAEELSSQSESLRASVLALEALVYGSAQKAPQQESSYAKAPAHKSKSSSPSAKV
                     LNFKKPAASAKKKAVTSSSEMIPFDEDEVSSARKVGTTDGF"
     gene            complement(33101..33829)
                     /locus_tag="VIG33_13990"
     CDS             complement(33101..33829)
                     /locus_tag="VIG33_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8271997.1"
                     /translation="MKMAFNSRGMGIIEVMIAIGILSIAMMGMLTMQASMQKETRALT
                     EKLGSLDLEKFLISALADGKICTAELTPSVINGNNSFKVNAGQPETTTFTLANGLHSN
                     AMDSTKILIGPGDHPSPFTKNLKVKGGGITITDFVKIDTNLYSANIKVEFESDGLVRP
                     LRPITVKTTVLTNASDTILGCGVGDVGFGGTYDACNCVSWGRCKGNHLNNDQFSCPAG
                     YTAYLVSTFDDRGKCGHNIYACYK"
     gene            34061..36952
                     /locus_tag="VIG33_13995"
     CDS             34061..36952
                     /locus_tag="VIG33_13995"
                     /inference="COORDINATES: protein motif:HMM:NF025464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparin lyase I family protein"
                     /protein_id="HEY8271998.1"
                     /translation="MRQTIARAIFSLSFLLCMILNISCSGSGSGYGSSSSYSRTASAS
                     TITAARAAPGSAQEALADRVQKLFSAKCLSCHNAQKASGNINLSTATAILQRGLVIKG
                     NAAGSQLYQSLVANRMPVGGGLDDASKKDVQDWINQGPFEFPSTFNGSLSVSKQEIRP
                     AISSSSFFGDWESGTLTGSGNYNWSQSQISTKSSYTLLNDGGGRQGSYYARVSVAPRR
                     CRSCVERSEVVQMQDAQNHPIVENESSEIQRYSFSVKFDSSWKPIVGNRSEAWGIFLQ
                     LRSPNTFPTRAAWAFNASNTITFRQRGGNLRYNKGKIYPLSNNSLNTGKWIDFVLTIK
                     YAKDNTGFFRIHRRDEGETTFTEVLNVTEVPTLQTRDGVVADHVIRHGLSRNHHNFTS
                     ILYLDGFTRETVSPTPAPIPTPNPSPTPTPPPATNALADRVQKILIAKCVNCHNVQMP
                     SGNVSNLTSARVLLEKGLVTKGNATTSRLYQVVATNQMPLGIALDEASKKDIADWINA
                     GAPEFTSNLPQPTPTPPLPDPTRIPQSVLDLAADEETIYQDLQQIAAETRAFTRYIVI
                     TQNYNSTGILTAQKALFAKGISKTLNSLHWKNTISIPVALNPESTALRIDLRNYDLTA
                     AEWSQITATYPYLNQLKASSRWNAIVSATKSTKPIVRGDWFVQQVLQPTLYKQLLEIP
                     ENIQTLETRLGVNAEERLRSGAAIRAGLKDSGVSFSNRIAERYESSYGAYWKSYDFIS
                     SDGNQNIFRNPLGPVLDNFSQGIGFVHNGGEMIFTLPNGLHGYALTSATGNYLNTAPP
                     EIVIDTSRADKLVTNGSSCFTCHTQGLIPFEDEISAASNRGHLFNNKQLTAINRLYIG
                     KTKLEETFNKDMAAYVDALDKLQISPDSGEPVSTVNLFYARNLQIKDIAAELQLSVTD
                     LRKYLESSSGLRSQFDFTNSHRVSTVKITRALFEQRFNQILQEVAKLKP"
     gene            complement(36960..38450)
                     /locus_tag="VIG33_14000"
     CDS             complement(36960..38450)
                     /locus_tag="VIG33_14000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY8271999.1"
                     /translation="MRPFNSRPLKSKWLENSFKGFPKLMIIVLLTATSLVHAGEAVKK
                     TEPDVLIVGAGIAGLSTAYRLKKEGKTFLVLEMSSHIGGRIRTASYTEDVSAEVGLEE
                     FWEGNPSLEIFKELKIPLEKSATSFSSFIYKGKLHPFTQNTNLEFLASFFNAKEMKEY
                     ENWDKKTADIYKKLLSNPKDKSVLFLKDLSFADWIQKTCKLSKKVQELIRLESEPEYG
                     TPWSRISAMDGIAEWHIFSGAGSSSFHVIGGNHKAALAMADSIGKEHILLNRQVTNIK
                     SSTDGVEVIATDSDFKQHTYHGKYVVTTPPLFRLNEIQFEPAIATERVQAIQSQGWGA
                     YFTAHLVLDEKATHFWTVKGESILPILTDSSLGVIYEGKSEGDKKNTMLNLLITGDPA
                     EGFNARVGTLDDVRAVLLKDFEKLWPGIGKHIKKIVFYRYHPRAIASWPVGRSRFDDL
                     SDSLRKPQGRVYFGGDFTEGTHSDGAAMSAIRIVKDILQKEKALQK"
     gene            complement(38468..38944)
                     /locus_tag="VIG33_14005"
     CDS             complement(38468..38944)
                     /locus_tag="VIG33_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272000.1"
                     /translation="MRKKLSALNLIALSWLFLLCACSEESKVREAAIGGAREQFQTEM
                     KAEIAKGVKGKIQTQATAARVLTDRSEFKAQKVEINGERAQVLVEVSAIPVQARTALI
                     EIIEKFDEKKEARFNAADALKLILQQMDLMETRSSYAYPMKLEKTDGWQVVKEKNK"
     gene            complement(38954..39679)
                     /locus_tag="VIG33_14010"
     CDS             complement(38954..39679)
                     /locus_tag="VIG33_14010"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEY8272001.1"
                     /translation="MEVVENQFQEQVKIFNKLMHSQSQRLSLEGVHDLRVSTRRLRSL
                     IDLLEKSTAHKVPKAAKKSLKELGDLLGDRRQWDVALQGARRYHLNTQGLLKDQRAAG
                     SKLHKILQRPEIQKIPHELRIFGEGLGVDRVPVRDKLLQKVSTELKQWIRQKKFSKKE
                     IHLLRISMKKIRYTFEFLDLPVNDLKDLQESLGQCHDLTVLSDYFGRPKKVKKDEQKK
                     YKKIQKRIRPALRSSLDVLGSVR"
     gene            complement(39686..40498)
                     /locus_tag="VIG33_14015"
     CDS             complement(39686..40498)
                     /locus_tag="VIG33_14015"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272002.1"
                     /translation="MSHSGKLSKLLLKRDFPHLRGSKETDQHLKDLQLKILRIQQGIW
                     HQKKRAIILFEGFDAAGKGGAIRRLVESLDPRGFRVHAIGPPEGDEQGKHYLYRFWTR
                     LPSPGHIAIFDRSWYGRVLTEKVNRLAPKTRLEDAYGEICEFEKMLIADGVDFIKIFL
                     AIDKDEQLKRFEERLKDPYKQWKITPDDVKAQSQWTEYVRAADEMFKRTSYKGGTWDL
                     IPANDKDYARLEVLKVVTKSLKGHKAWIDSLSQKVTMLTLSSALKELGVKKI"
     gene            complement(40528..40995)
                     /locus_tag="VIG33_14020"
     CDS             complement(40528..40995)
                     /locus_tag="VIG33_14020"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEY8272003.1"
                     /translation="MKGTMKTWVAVMNRTEARFFEINEKEKNKLKLLKKVANPRGRLK
                     NSEINADRPGVNKFSFAYSQTSLTKPQKPTDRVAQIFAKDVSEELERGFNAHLFQNLI
                     LVAEPHFLGKIRAALSKKTFETVSSTLPKDLVHVSDHDLSQLIWHHMESTVSL"
     gene            complement(41025..42056)
                     /locus_tag="VIG33_14025"
     CDS             complement(41025..42056)
                     /locus_tag="VIG33_14025"
                     /inference="COORDINATES: protein motif:HMM:NF015254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectin acetylesterase-family hydrolase"
                     /protein_id="HEY8272004.1"
                     /translation="MKLFLIILLASHLSYALPSGWQEIKPGGETLCARGGEFAFLVRK
                     GDPQKIVVSFAAGGACWDDFTCDSTKIYTDTVAKTYGQVNRQAGIFDFQNKKNPYKSW
                     THIFIPYCTGDVHLGTSDATYTRPDNSSYVIHHRGAINTKAVLKWMMENYKTAAEINV
                     DGCSAGSYGSVVWTPTIAENYTQARILQFGDSGAGVTPSLFFPQWGVEKALPPWIPAL
                     NPGSVQWSKLNIVDIYKAVADYYPQARFSQFNHEEDRIQTLYYAAMGGNGLEWTSRML
                     KNMGETSSLVGRFHYFIAPGITHCSVNNASFYSTKSDGTILSDWLSSSIAGKDSENVK
                     CLECKTKIE"
     gene            42210..43310
                     /locus_tag="VIG33_14030"
     CDS             42210..43310
                     /locus_tag="VIG33_14030"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY8272005.1"
                     /translation="MKQWVKKLVDQLDWKETDASTPSPHQDLTEDRATLLYILDNYSK
                     HLFEIDKQPLRRVRETFDSFVKGLVSPDPKSEKLLFQLRQFFSSYRIDEYTYIQNTFD
                     DFKRIIWDFAEQLSEDIEYENAKDAETSQSLEELRGAVDANSIEALRSKSREFIDYYI
                     KYQAQKNERRSKRLTTMKKNLSSIKKQLMEANQNMRVDHLTSAFNRKSFDEQMKKHLQ
                     MAAISQTPVTVMILDIDFFKKINDTYGHDIGDYILKECVKMLKEVFPRAEDFVSRIGG
                     EEFAVILPNFTAEHAIVRAEETLQKIRKEVFVHGELKICFTVSIGIAQWLHNETLDQW
                     IKRADSALYHSKQTGRNKYTVAPPERGIKQVA"
     gene            43446..44441
                     /locus_tag="VIG33_14035"
     CDS             43446..44441
                     /locus_tag="VIG33_14035"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HEY8272006.1"
                     /translation="MSRMKTFLLWALFIAPLTGPFSAKADVNMKDASYFKTWIDLEIR
                     KDTHIFQLRRTYNSRSLHPGLFGFGWCSDFEKSLDLNRSDQILLQDCRLNAPIRFKKI
                     KENLYESPNKETLELKENHYLRTTTEKTFQKYNKLGKLLSLIDTAGDHLDLSYSGNGF
                     LQKIIFKDIEQNINFDLNHRHIQVIKSKNGQEISYRYENNNLTQVNNSWKNIFLYSYD
                     EFHNLTRIQAPQGGQEVLTYDAEKDWILRIDSANGTLNKCAEIFHYYQKKDMSYVSTA
                     EKRCAGKSIAKTIYEFRYRKRADGSKYLEQVNVTQGNKIQRISYQNKPGLLNESP"
     gene            44428..45549
                     /locus_tag="VIG33_14040"
     CDS             44428..45549
                     /locus_tag="VIG33_14040"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEY8272007.1"
                     /translation="MNLHKLKILFFTLFSFSSLGFASPPEGLQMAEARRARPLNQGSK
                     DIVVAIIDTGMDTLHPSLQGNLWTNFGETGIDSHGKDKSQNQIDDDGNGFIDDVHGWN
                     FADNSNDLSDLHGHGTHVAGIIHSIAPKTNIMILKYYDAKASGAENLTNTVKAIRYAT
                     QMKAKIINYSGGGTNKYADEEVAIRKAQLQGILFVAAAGNEKSNSDISGFYPANYGLS
                     NIISVTAIDESGKVLPSSNYGSHSVDIAAPGKRIVSTLPGGKFGAMTGTSQATAFVSG
                     TAALLLAEEKNRKAPERLIQQLTQSGNTNDFLIDKTKHQTTLNIYRSLIMKDSNLGAF
                     GNVDETGWYSSTDFSSKLNENSEDFGSAELPFKIQVNRK"
     gene            complement(45553..46767)
                     /locus_tag="VIG33_14045"
     CDS             complement(45553..46767)
                     /locus_tag="VIG33_14045"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impB/mucB/samB family protein"
                     /protein_id="HEY8272008.1"
                     /translation="MVRWLYLDLNSFFASCEQQESPELRGKPVAVVPMMANTTAVLAA
                     SYEAKAKGIKTGTLVGEAKRMCPGLKLVQARHKTYVGYHQKIRDVVESCVPIHSVCSI
                     DEFACELTGSQQDLAVATTLAMKIKKEIRERVGVCMTSSIGLAPNMLLAKMATDMQKP
                     DGFITIEPEELPQRLFPLKLRDIPGVGPKMEQRINQKGITSMQQMLGLNEKQMHGLWG
                     SLVGSRYYYLLRGENILIPRNKSQSISHQHVLPPNVRSWEGALGVAQKLLSKTAVRLR
                     KENFLARRLSLSVKFINQFEKFERSAKLQETQDTTLLLSHLHRFFAEMSGNCRTAKPL
                     MVSVVLSDFIKSENHQFSFFENTRRERLFGVVDKINEKFGKDTVYLACLQDKKDVAPT
                     AIAFSRIPELEE"
     gene            complement(46861..47451)
                     /locus_tag="VIG33_14050"
     CDS             complement(46861..47451)
                     /locus_tag="VIG33_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEY8272009.1"
                     /translation="MSYNPQDHYFKKAKEENYAARSVFKLEEIDKKYKLFKPHQVILD
                     LGASPGSWSQYASQKTGAQGRVLGVDLKPVTVHLKNAVFIQADLYDLQLEEIFKEHGF
                     HPPFDIVMSDMAPNTTGHRFTDQARSMALCELALDVARRFLKPGGHFVCKLFHSDEFA
                     KLREEIKKDFQRFEAVKPDSTRKISKEIFLVGINKR"
BASE COUNT        12918 a        11676 c        10247 g        12921 t
ORIGIN      
        1 aaattggctc ctcaaagagc tggcgaaaat aaatagggct tcgccaaaaa atgttaaaaa
       61 accgattttt atcactggac atagtcttgg tggagccctg gccaatttaa ttatgtactc
      121 cctgttagaa cataatgatt cgaccaagaa tcagaattca cgattaaatc tcaagtcgct
      181 ctacactttt ggtgcgccac gagtgggtga tcagatttgg gcaaaaagat ttcatcaact
      241 ggcggagcaa atggatgtag gcttatatcg catggtgaac ttcagtgacc cagtgcccag
      301 ccttccttgc ttaaactatt ctcatgcggg ctcaatcgta ttcatggact taaactcaaa
      361 tggatttagt aaagttattg actcggtaaa agtaatttta aatcccttga atcgagtgct
      421 taacctaggc actacccata tcaatctcac caactgtgcc tatgaaacag tcatctacta
      481 tccccgccat attaaggaaa tgataaataa tcactttatg acttcttatt atcaaagcct
      541 gtctttttta aaaaactccc tcaatgaaaa aatggaaaca caaagaaaac aagcactctt
      601 taaccatgat tataaaacgg cagaacagtt cacttacccc tcgaattgtc aaaaacctaa
      661 tgtcagcaac ttaaatcttg agggtttaga attgaattac cagttactta aaaataattt
      721 ggaggattaa tttattttat tgatgcgatc aaaaggaata acccgcgcca aatccaaagt
      781 tcgtcgtgcc atagtggaat ctaaattggg agccaaggtc ttcgagattt tcagaggtcg
      841 taaagacatg catgtgtcca taaagctgtg gtgtaaaaaa cattttaatt aaaaactgca
      901 caaaataaga attctgaatc gtgacctggg aatccagtgt tcccgggaaa aaatcacctc
      961 ttaatagaaa atgtttttca taggataaaa atggaatttt tttaaagaaa gcattcaggg
     1021 actcaggaaa agcaccctca taacgcaagc cgattcccat caataccact gaaatgtcat
     1081 cttgtttgac attcttaata cgccccgaag cgaccaaacc caaacggtcc ttaaagtcct
     1141 tgctaaaaaa ttctcttccg attccagcat cccaaatggt ggtttccgga agtactaaag
     1201 acttatcgaa attaaagtat cttaagtccg cctgaatctc gttccaaccc cgaagaaatt
     1261 caatattctt aaaagtcttg agttcgattt tttgtggcat gaagctttca gtgccatcat
     1321 cagcaacgat gatttgaggg ctgctttttg ttagaaagaa acccatccag acattgaggt
     1381 cattataagt aaagacgggt tccacaggag tggttaccgg tacttcttcc ggagtttttg
     1441 cgggagtttc atctaaaggc ttctcaaata tattttcatt tatgagcagt acggaaatct
     1501 gttctgaatc tttggccgta ttccgagtct tgtgaacagt cagcgaacct ttttttaaaa
     1561 aatcctgagc ccaaccgcgg aaagtcttag gctcaatatg gaataagtaa ccttgagggg
     1621 tgatttcgaa ttgaaattgc cgtcgccatt gactttccaa tgctttcgaa tcctcggttt
     1681 cagcagtcca taaaatgaca taatccgctg aggagccctc agtttccacg ggagcaatgg
     1741 aaggtgcttt tcttttggaa aaagccactt caaatctgct ttggtcccaa tgaggatttt
     1801 tttctaaaat aaacggaccc tcattcggaa ggatttcaaa tattttttga aagttttcat
     1861 catctccttt aataataaaa gaatttatac cagtaacctt gccctcggga aaaatccttt
     1921 gaagttcggc gatttctttt gagggattcc gtctactctg atgtaaggaa agaatgtctt
     1981 ttttttcctt aggacgagtc attcgcagaa tgactcgcag agtccctggg gaagaaactt
     2041 tttcactgag ggatttttca atcgctaaat cccctttttg cataagctcg cgatacaagg
     2101 actgccaatt ttcctgactt acgtaaaagt gaaagtaagg gcctccttga tagacagccc
     2161 caagttgtaa gttccctgct ttttgaactc catatttttt taaagtttct agaacttcag
     2221 aatgatcctg ggttgaataa taggtgccgt tatacagaaa agtgctcgcc aatgcatttt
     2281 ggttcgatat taagaccgca aatgtgatga gcccagctag ttttatgaac attaaaaaaa
     2341 acccctgaat cttaatcatg cggatttctt ttatgagtcc caagcaaaaa atacttactg
     2401 gaaaatggtc cagtgttttt aaactcatta tcttactgac attatatatg ggttatggaa
     2461 ctggctcgaa cttcactcca tcaacattgg caatgccggc tgtatccttg aactccacaa
     2521 aattaaggtt tccagcactg aaactataag tcccgacgga agtccaaccg ccgccaccgg
     2581 atcgcatatt caaacgttta gtaacgctgc ccgtagctga tttgatagtc accggaactt
     2641 taccgctaaa atttttactg gagagataat gtaccgatat ttgataattt cctttcgctg
     2701 aaatagaagg tttaaagcgg taaatatccg ttcctgcacc acaactgatc agggaagtga
     2761 tgccgtaaga tcctgacaaa tttgaactgc accattttcc gataaaactg tgagagctgt
     2821 cagaaattcc tggagtcaaa ttatccaaca gaatcgcggg agcaacagcg gtctgattat
     2881 tcacgttcag tgtgatagta ctcatagaac tattgccgct ggcatccacc gctttgacag
     2941 ataagctatg ggcgccattg gttaaggtta ttgtatttaa accgaatgta taggggactg
     3001 cggtgctagt gccgagcaaa ttggcatcga tgtaaaaagt gaccgctgca actcctaggt
     3061 tatcggacgc tgacacttga atgttaagaa ttccagaaac gatggcattg ttcgcgggaa
     3121 aagcgatcgc cacgctcggc gccacattgt ccgtgagaac cgccgccgca gctttctgca
     3181 cagccaaacc tgcacttata cgaccgccag cataggtcag atcataaccg gttgccccca
     3241 gatcttggac tgttgaaagc aagaaatttt caacctgatc aggcgtaaaa tttggtttca
     3301 gggaatagag caaagccgca gttcccgcca ctataggagt ggaaaatgaa gtgccactca
     3361 ccacctcata accccccgga atggtggtgt aaatgttctc tccgggagcc actaggtcca
     3421 caggggtgcc atgattggag aaactcgcta gagcgtctgc agagtccgtc gcgccgacaa
     3481 ttatcatgtt gggagaagcc ccccaggacg aaatatccga gctgctattc ccggccgcgg
     3541 caaccagtaa gccgcccgca ttgcgcaggt agagagccgc atccttaacc acctcactgg
     3601 cataggcata gtcatagctc agattgacca cttttgcccc ctgatcactg gcatattgga
     3661 gacactctgc aacgtctgaa taggtcgaaa ccccatcccc ttgctcactg atctgaatag
     3721 gcagcaggcg tattctccac gccatccccg ccacgcctaa actattattt cccatggccc
     3781 caatcacccc tgaagtcatg gttccatggg gatgcagatt agtgacttcc gtattgtttt
     3841 ttacagtatt ataacccggt aaaataaact gcgaagccaa atccggatga gtcaaatcaa
     3901 agcccgcatc acaaactgcg accaccacag cggagttgcc ttgggtgtaa ttccacgaaa
     3961 gctcagattg aatctttgcg agatgccatt gatttgcata aaaaggatca ttgggaacga
     4021 aagcgtgcgg cacgatgtaa tcaggctctg aaaattgtac ggcctcggtc ttctttatct
     4081 gttctacaat gtcttcctct tgatcggaat aggagttgtt gtgaatacga agtctcacaa
     4141 ccttaccctt caggagcctt ttctcagcac gcaagtcaaa ttcttcataa agggccttta
     4201 acgcggccaa ttgctcggga gtggggtttt cacgaagctt tattagaact gtattttgac
     4261 gacggtggcg acgataattt cctcgtccca aagacgccgg cgcccgggaa gaaggttgcg
     4321 agtatatttg agaaggcaca gacactggag aggatataca agcggttata gccagactgc
     4381 tcactaacac tgtaaatacg gaaaaaacca tgacacgtgc ttgtggtttc ataaatgttt
     4441 tcttccaaac tataattaaa tacaaagtta tttgtattta ctctgaagct actcacagga
     4501 ttttacaatc cgaccttggt ccataaatat tcggtaaatt aatttttatt gaaaacttct
     4561 agcaaagcga aaaccctcaa gacaattcaa aactatattg tacctcgact ctgttcttca
     4621 aaggagatcc taatgggcgt atgcgaaact tgcggcgcga gctatgaaag aagcttcaaa
     4681 attatcatca acaatgaaac ccatgagttt gactcattgg aatgtgccat tcagggaatc
     4741 gctcccacat gtccccattg cggatgtcga gttataggcc atggagtgga ttcggatggg
     4801 gtcacttact gctgtcatca ctgtctatgg atcggaaaaa ccaagagctt tgacgaggaa
     4861 tctcctccta tttaatgagg ttcactatca ggtcttctgt gcggagaaat tcattgaaga
     4921 attttttgag ttcttttttt aaacaattaa ataaaactga tgacgacttc agagtttttc
     4981 aagagcctat tcgatctgag atttacagtg ccacccgctt ggaaagccaa gcggagatcc
     5041 ttgctcactc ccatttagta tcgaagactc cacgaaaagg acaccgtctg gggccacgca
     5101 tttccgccaa taaacgtttg ctggagaagt cttacttaaa cctgattcac gccgtcgacg
     5161 agcaccgggc tattaccccc gcagcggaat ggctgatcga taactttcat atcgtgcgtg
     5221 cgcagcttaa agacatccgc gatttaatgc cccgagaata ttatcaggag ttgccgaagt
     5281 tgttgatagg gccccttaga gactaccccc gggtttatgg gatcgcgtgg tattatgtcg
     5341 cccataccga cagccacttc gatgccgatt ctctgaagcg tttcttattt gcctatcaaa
     5401 aagtacaacc gctcaccatc ggcgaactct gggccctgcc catcacactg cgagtggtcc
     5461 tcatagagaa tcttcgccgt ctcgccgccc gcattgtggg ttctcaagag gctcgccaac
     5521 aagccgatca aattgccgat gaagttctgg gactgggaag tcaaccgcct cgtctgatcg
     5581 ttgaaatcct cgcggaactt gaacgaatcc ctttttccat ttggttggcc gtacagctcc
     5641 tgcaaagact gcgatttcaa gaccaaaaag tggaacctct cttaaaatgg ctcgaccagc
     5701 gcctggaaaa aaaacatctc acctcagaca ccgcagtcac tattgaacat ggccggcaaa
     5761 cagccgctaa cgccaccgtt cgaaatatta tcaccagtgc acgtttaatt gcggctttta
     5821 actggcaaga tttttttgaa gaagtcagtt tggtggatca gattctgaga caaaaccctt
     5881 tctattccct catggatttt gccacccggg accgttatcg tcatagcctt gaacatttgg
     5941 ctcgtcattc tccttactca caaatcgaaa tcgcgcataa acttatcctc ttgtgcgaaa
     6001 aaggaaaaaa acctggtgat ctcagatcct ctgagcttgg ctattacctc atttctaaag
     6061 gacgcaaaag cctggaaaaa gaaatcaaat ttcgtctgac ctggatggag aatctgaacc
     6121 gtggttattt tgaaagtaat accgagatct atctcggagg aattctcttt gtaagtctct
     6181 tgcttttgtc cctgccgatc ttggcgacac ggaatcttga tctcgaccgt tggcaagttt
     6241 acattttcgt tgccctcggt attctagtgg cttcggaagt cggcgtggcc atcatcaacc
     6301 gcgtaacggt ggctttactc ggtcccaaac atctgccccg tatcgatctt gaaaaaggta
     6361 ttcccgaaag ctcaaaaaca tttgtagttg tccccacctt tttaaatcaa gcagaagcca
     6421 ttgattctca gttggagcag ttggaaatcc actatttatc caatcctgaa ggacatcttc
     6481 attttgcttt gctgacagac tgggtggatg caaccacaga atatcttcag caggaccgac
     6541 ctcttctgaa tctggcggct gaaaaactca aaatattgaa tagcagatat ccccctgcac
     6601 ctgacggaca tccgagattt catatctttc accgcaagcg tctttacaat ccccaggaga
     6661 aaaaatggat cgcttgggaa agaaaaagag gcaaacttca cgaattcaat cgtcttttat
     6721 taggagctca ggatacaagc tatattcctt tggaaatgaa tggacctatt gaagtgccct
     6781 cggaggttcg ctacgtcatt actctggatg cggatacaaa aatgccaaaa ggctgcgtgg
     6841 ctcaactcgt tggaacgatg gctcaccctc tgaatgaagc cttttacgat gatgataaag
     6901 gtcgagtcac ggaaggctat ggaattctgc aaccacggat cacccctacc ttgccaagca
     6961 cgcaaacagg cacgcttttc caatttctct ccgcgagcac tgcgggtgtc gatccctatg
     7021 cttccgctgt ttctgacgtc tatcaagatc ttttcaatga aggatcttat actggaaaag
     7081 gcatttacaa tttgcgtatg tttgaaagag ccctggaaaa acgcatccca gaaaattctc
     7141 tattaagcca cgatttattt gaaggaaact atgcccgctg cggatttctg agtgacgtgg
     7201 agttcttcga agattttcct ggtcactgct tagcagcgtc cctgcggacc catcgttgga
     7261 tgcgcggcga ctggcaattg ctcccctgga ttttaggccg agggggagcc tctctttcga
     7321 ctatcggacg ctggaagatg gcggacaatc tccgtcgctc tttatttcag cccgcagcct
     7381 tttttcttct catctttgcc ttggccacct cattacaatt ggcttggatc tggggcctat
     7441 tcaccttatt ggctctggcc aaccccccgt tgattacttt tgtgactgat ctcttcaata
     7501 aaaaaagaag tgtgcgtgtc ctggaacatc taaaattcgc cctcactgac tttcaggtgg
     7561 gactgcttcg aacactcttt tttttccttt tattgcctta ttacgcctgg ctttccggag
     7621 atgccatcct gcgtactctt ttccggcttt ttattagtaa aaaatatctt ctggaatgga
     7681 ttccagccgc acaagcagaa gcggccacca gtctaaacat taaaagtttt ttttacgaaa
     7741 tgcgcggcgg actgattctg acggcgtttg ctctcccagc cgtcgtcctg tttaatcctc
     7801 caggtttatt tttagggatt tttctttttt taccctggct gctttcgcct tggtatgctt
     7861 actggatcag tcgtcctttc gagaagaaaa cactcagacc catgaccagc tcagatcagg
     7921 aaattcttcg cctgactgga cggcgtatct ggcttttttt cgccagcttc gtaaagagcg
     7981 aagatcactt tctgccccct gataattttc aagaagaacc ccatcccgtg atcgcccacc
     8041 gtagctcacc gaccaacttc ggcctttacc ttctctcggt cttttccgcg cgcagctttg
     8101 gctggatcac tcttcacgac gccattcacc acttaagcaa aaccctggag agtctccatg
     8161 aattgcccaa gtacaacggc catttttaca actggtacga aaccaccacg ctccgcccct
     8221 tggatccgaa atatatttct tccgtcgaca atggcaactt agccgggcat ctcatcgccg
     8281 ttgcccaagg cttcaatcat attctcagcc aaaaaatcga catggcaaat ctgacggtag
     8341 gagtttatga aagcttagaa ttattaaaac aggcgctctc gtcaaaaaag agcccccttc
     8401 tcacgagcgt tcaccaaatc gaaaaagcac tcagcctgag tcgagacgag cccatggatg
     8461 atgccaaaat gaaaaatctg aaaaaccaat ccgccacgat tctccaacaa gcggcgcatt
     8521 cttccaatgg gtccggcgat ccggaaacct ttaataaaac tctcggtgaa accctgacgt
     8581 gggctcaagc tcttcatcag gatatcgtca gcctcaacgt tgagtatcat tctttattga
     8641 gttggtttga ttttataaaa aaatctcttc caggacagaa tcctcctgag attctcagcg
     8701 cctggaaaaa aattcagctg aaattgtccg ccgagatctc tctggagatg attccagacc
     8761 attgcgcggt gatcagcgag aaaattatcg actttgagaa gcaaatcatt gtccaaaact
     8821 ccgagactgg gaccaacgcc gaaactcgac tctttttagg gaatttactc gatcgtctcg
     8881 acaccagcat gcatgcggca aaaaccttga ttgccaaggc taaatgctcc caagccatcg
     8941 cttatcagct ttttaatgaa atggattttc gtcttctgta tgataaaaac agaaagctct
     9001 tctctattgg gtatcgggtt tttgaaaaaa ttcttgatcc tagctactat gaccttctag
     9061 cttcggaggc gcgattgctg agctttgtgg ccatcgccaa gggcgatgtc cctgtttcac
     9121 actggttcag cctaggtcgt agcctcgctc aggtcggaga taaaaaaggt tccgtcttag
     9181 gtgccgcttt agtctcttgg tctggctcga tgtttgaata tctgatgcct tctctggtga
     9241 tgaaaacccc cgaagggggc cttatcaaaa acacctgtga gttggtcatt caccgccaga
     9301 tcgcctatgg taaagaacgc tccgtgccct gggggatttc cgagtccgct tacaataaac
     9361 gcgatttgca tttaacttat caatactcta atttcggcat tcctgatctc gccttgaaac
     9421 gaggcctggg cacgaacatt gtggtggccc cctatgccac gctcttagcg gcgatgtatg
     9481 agccttcctt ggctgcggaa aatcttcgac tcttgaaaac catcggagcc tgcggaacct
     9541 atggttatta tgaagcattg gactacacac gttcccgtgt tcccacccat cgcaattttg
     9601 tcgtgatccg cgcgtatatg gctcatcatc aagggatgtc actcgtgtcc ttggcgaatg
     9661 tcttctataa tgaacttctg tgtcgacttt tccataacga acctctcgtt caagccactg
     9721 agattctgct ccaggaaaga acgccacggg ccgtaggtgt tcttcccacc tcggaggatc
     9781 atgctcccct cttcattcgt gaagataccg gacatctttc tcgccgctat cacagcgtca
     9841 atcttccgac accgaggacg cagattctct cgaatggaaa ttacacagtg atattgactt
     9901 ccgcgggctc tggctacagc caatatcaaa atctagcagt gacacgatgg cgagaggacg
     9961 tcactcagga tccttggggc agttatattt ttattaaaga tcttgaacgc caaaaaatct
    10021 ggagccccac ttatcaacct ctgggcactc ccccggaaga ttatgaagtc acctttgccg
    10081 aggatcgggt tcttttccac cgcgaagagg aagagataga atcagaactc gaaatctttg
    10141 tgtctccaga gaataacgcc gagatccgtc agctcacttt gactaataag agtgaatatt
    10201 tccgaaatat tgaactcacc tcctacgcgg aaatagtttt aaatacacca gctgcagata
    10261 tggcccatcc cgcattttcc aatttatttt tacaaaccga atatttggcg gcaactcaaa
    10321 ctctgctagt gcgccgccgc ccccgatcct cagaggaccc cacactttgg gcggcccatg
    10381 taattgtcgc taccggtgag atcacatacg agatcgaata cgaaaccgat cgcgctcaat
    10441 ttctcggacg aggaaggacc attcactatc cccaggcgct cttcaagaat caaaaacttt
    10501 ccaaaactgt gggcgccgtc ttagatccca tctttagtct gcgcacgcgc ttgcgcttgg
    10561 agccgggcac ggccacgcgg gttttctttt ccactctcgt cgcggaatca cgaaaagaaa
    10621 ttcttcgcct cgcggaaagt tttcaggagc tttctaccta tgaacgggtt tcgtctctgg
    10681 cctggtccca ggctcaggtg aaacttcact atctcaacat ggaacccgat gaggcccatc
    10741 tgtttcaacg cctggccacg cgtttgcttt atctggactc ttcgctacgt ccctccaatg
    10801 cggtgctaaa acgcaatacg caaaatataa ctcatctctg ggcccatggg atctctggag
    10861 attttcctgt gatcgtggtt cgcattgaag acgccgagga tcgtggtatt gtccgtcagc
    10921 ttttaaaggc tcaagagtat ttggccttga aaagatttgt cgtcgatctg gtcatcctca
    10981 acgccaaaag tagctcttac agtcaggaac ttcaagaaac cctggagtcc atggttcatt
    11041 cgcggttgag tgctccgctg cccttgcctt actctcgagg gaaagttttt attctgcggg
    11101 aagagcccct tccccgagag gaccgcttgg tcatttatgc ggaagctcgc atcactttat
    11161 cggcgggtca tggaagcctc tctgatcaaa tcaatcgcat gcttcaaccc tccgttcctc
    11221 cttttaccgg cagagtttac cgcgacgcca gggctgcagt atttttacct ccgccccccc
    11281 tggaattttt taatggcttg ggaggcttca ctcctgacgg tcgagaatat gttattgttc
    11341 ttaacaattc ctcgcagaat actccggcac cgtggatcaa cgttatttcc aatggaactt
    11401 tcggcttcca ggtctcagaa agcggatcag gatttacctg gtctttgaac agccgcgaaa
    11461 atcaaatcac ttcctggtct aacgatccag tctgtgatcc cagcagtgaa gctttttata
    11521 tttatgatct ggattccaaa tgcttatgga cgccgacggc tctgccgatc cgcctggaag
    11581 aggcttccta cattgccgct catggccagg gctacagccg cttcgagcac ttatccaatg
    11641 acattcacag cacgctgact cagttcgttc cctttggcga gaaattagat tccgtaaagg
    11701 tatcacaact gcgcttggaa aatcgctcgg agcaatggcg acggctgtcc ctttatgcct
    11761 atgttgaatg gaccttggga tttttgcgct cgactacggc tccttacatc ctgactgaaa
    11821 tcgatgaagc tagtggagcc ctttttgcga ccaatgtgcg aaacagcgaa tatggccacc
    11881 gagtttcttt tgcgatcttt cccaatcgca aacaacaagt caccggggat cgcagggaat
    11941 ttatcggccg caaccgcaac ctggccgcgc ccttgggaat tctgcgcgac cagccgcttt
    12001 ccaatcacgt cggtgcggct ctggatcctt gcggggcttt gcagattgat gtgcaaatcg
    12061 gcccaggaga agtattagaa gtacctttct ttttaggaca ggctgccaac cgcgaagaag
    12121 cccgctctgt catttttgaa ctgagcagtc gatcctgcgg cgatctgctt aaaaaagtga
    12181 gtcagcaatg ggatgagtgg cttgaacaaa tccagattga aacccccgac aaagccttca
    12241 attttttgct gaatcgctgg cttccttatc agaatctttc ctgccggtat tgggcgcgtt
    12301 ccgcttttta tcaagctgga ggcgcatttg gcttccgcga tcaactacaa gacatgatgg
    12361 cgacggtttt catggctcca caaatggtgc gcgcccatct cttgcgggcc gccagccgac
    12421 agtttatcga aggcgatgtt cagcattggt ggtttccctc ttcggaccgc ggcgtgcgca
    12481 cgcatttttc tgacgatctc ttatggcttc ccttcgcaac tttccattat gttgaggtca
    12541 ccggagatct ttctgtgttg gatgaaataa tttcttttct ggaagctccc cttctcaagc
    12601 ccgagcaaga ggatgcctat gacgtccctg tggtctcagc ccaaacaggc agcctctacg
    12661 agcactgtgt gcgagcattg gagcgcagta tggcccttgg tgcccatggc ttgccattga
    12721 tgggcggcgg tgattggaac gatggcatga accgcgtcgg caaaaatggc aagggcgaaa
    12781 gtgtttggct cgggtggttt ttatatttga atttgcagca attctcacat ctcgcgcaaa
    12841 cgcgcaatga aagcgcacgc gccgagatct ggctacgcca cgctgatcaa ttgaaggcag
    12901 cgctagagcg tgaagcctgg gatggagatt ggtatcgtcg cgctttttat gatgatggca
    12961 cgcccctcgg caccgctggc gatagcgaat gtcgcattga ttctctggcc cagacctggg
    13021 cggtgatctc cggggcggcg gatctagccc gagctcgcca tgccatgcaa gccctcgaca
    13081 atatactggt tcgccgccca gaaaaattag tactgctctt tcatcctccc tttgacaaaa
    13141 cacccctcga tcctggctat attaaaggtt acgttccagg ggttcgcgaa aatggagctc
    13201 aatacacaca cgccgctgct tggtgtgtga ttgcttggac acttcttgga gatggagaac
    13261 gggctcttga actctttaac ctcctcaacc ccatcaatca tgcgctcagt ccggatgagg
    13321 tgcgacgcta caagattgaa ccctatgtgt tagcaggaga tgtgtattcg caagaacccc
    13381 atattggccg cggggggtgg agctggtaca ccggctctgc gggatggatg tacagagcgg
    13441 ctttagaata tatcctcgga tttcgcctca aaggacagga gctttttttg actcctcaaa
    13501 tttccccgca atggagattc gtaaaactgc gctataaata tcgccgttct gtttatgcaa
    13561 ttcattttgt aaatccggaa gtctatacgc gaaaaggact gcaaatcacg ctggacggaa
    13621 cacctttagc cagcggcgcc ggcattccgc taaaggatga tgaaaaaatc caccaggtgg
    13681 tagtcacctt acagtgattt gagctgattc gcgcccatgc tgttgaggaa attcaaaatc
    13741 acgcaggtgt ttataaattt cttctttatc agcaccgtag cctacaactt tttgaacatc
    13801 ttccagcgcc tgaaacatca cagccaaacg tttttgatcc aaagctgtaa ttttcttttt
    13861 caactggttc acgtccgtag atttgtagtt ggtacttgca atcaatttaa ataacaaaga
    13921 tttgctctga ccgatttccg cgcgaatgct catggcttca gcatatgttt ttgcataaat
    13981 agccattagc ttctgctttt gttccgctga aagaccaggt gcccctgaaa aagcctcagc
    14041 ggcgcgttga gcaatttgct ctggcgaatt tgcctcaggc tgcgctttca cttgccgctc
    14101 aagctccgtt ttttttggtt ccgaagagca cgctccaagt agaaaagctg taagaagtga
    14161 agaagtcatt aatgcgaaaa actttttatt cacacaaaac attgtttcgt cctttgcatg
    14221 gggttggttt aaacttcaag caaatagtat tcactcaaga gtcggcaaaa gcaaaaccct
    14281 ccagtgtcgc tgcgcgcaaa cataaggaaa tacttcgatt cataattatg gagaaatcaa
    14341 ttaaacttaa gatatgccaa agctgaatca ttttgaactc gtcctcgctg ctattgactt
    14401 tattgagaat aatttaaaag aagaaatctc tattcaagag gtttgcaaag aagtccctct
    14461 ctccagttgg caattccaaa gattgtttcg agctttggtc ggcaactcca tcgggagtta
    14521 tttgcgcggg cgaaggctca ctctggcggc tcatatgcta gccgattcaa agagaattct
    14581 agatatcgcc attgacctgc aatttggctc ccaagaggcc tttacccgtg cttttaaagc
    14641 ccaatacagg atcacccctg ggcaaattcg caaccatcct gaatatatta aactctataa
    14701 gaaacccaga ctgaccaaag acaccctcgc tcatcttcag aatggaattc agcgagagcc
    14761 taaaatctgc ctgttgggtc ccttcaatct cctaggcaag gtaacgacca tcacttcctt
    14821 tttgggaaag aatccagact ttgaaagcaa gctgccgggt ttctggaaag aatttaatcg
    14881 ggaaaaagga gctcactcac tgaataacaa tacggagagc tatggcgtgg caatcagttc
    14941 cagccaagat atgtttgaag aggaattgaa atactttgct ggaattccag ttccggctga
    15001 gagagaaatt cccaaggaat ttgttccttt gcatatggac gaacaactct atgcagtctt
    15061 tgaaaataag ggattggcag ataagtccca actgactatt gactatatct acggtgtttg
    15121 gctccccgaa tcaggctatc agcgggccag gggctttgat tttgaaatct ttgatcaccg
    15181 ttacagttta gattcttcgg actccctttc tcaatattgc attcctatcg agaaaatttc
    15241 ttaaaaaaaa ttattcaaac cgcacaaatg atcaaaaaca gggggtgagt ttctgttaat
    15301 ttccgttctg tagataaccg gaacccgaaa ttaacgatcc cctgaaagga tatttgtatg
    15361 gaaccaaaag ttatgaatcg cagacaagtt ttaggtcttt cttcagcctc cttactgatg
    15421 ttggtctttg gagcaaaagc caaggcccat caaggtgata cagcagaact aacccaagat
    15481 tttttaaaaa ttgaatcggg accagggcag atcgtcaaca atagctttgc ccatcatcat
    15541 cactatttag aaattcccct tcaatatatt caagagcccc ctgctgcagg cctgcaaatt
    15601 catacgggat gggcgatttt ccgaaataaa ttcgctaatt ttgctcctca cttccacacc
    15661 gttcccattt cccagcaaga attagaggct gtgggtcgtg gcgaaaccgt gatcgtagag
    15721 gacactgtaa aggatcataa gtacgtttta actctctcct cttctatcac atcttggcac
    15781 catccagaac ggcacagatc ttgataactc ctctctatgc tagcctggcg gtatcaaatc
    15841 gatactgctt ggcagttttt ggaggggaca tgggaaaatt aaaaaaaaat ttaaaatcta
    15901 cgacgcttgc atatgcactt tcgctgtctg gtctttttat cactggctgc acagcatcga
    15961 atacccctga aaatgaattt ttgaagtttg gttttatgga gggaatctat tccacccgtc
    16021 cgcaaaccgc tgagccagtc attaccaccc tcaaattaaa aaatccggct ttgcttgaaa
    16081 gtgccgaaag aaaaaacgga gttctttctg ttgacaaaaa tctggtggac gccattcaac
    16141 aagaacaaac ggccacgatc gcggaactac aaaagatctc tccagagatt aaaattctta
    16201 ttcgctatcg attggtgctg aatgccctga ctattttagc tccgccagaa gctcttgata
    16261 aaattagaac ccttcctcaa gtagtgatgt cagaaaagtc ctccactttc gcacgacctc
    16321 tccctcttga tacagaaaaa aaagggggtc ttgtgggctc ccatacgtcg gtgaacttca
    16381 tcggtgcgga agcggcttac gcccagaata ttcacggcga aggcatgaag gtcggtatca
    16441 tcgatactgg tatcgactac actcataaaa tgttttctgg ggaaggcacg gaggaggcct
    16501 acaagaacaa caaccctgcc caagtgaatc cagcttttcc aggcagtaaa gttgttggcg
    16561 gcatcgacct ggtagggact gaatatgatt cagcctctgc tgatttctca caacacattc
    16621 cggtccctga tttaaatcct ctggatgaat caggacacgg cacgcatgtg gccggcaccg
    16681 tggccggaat cggcgatgga gtcaatacct atagcggagt ggcccctgcc gccagcctct
    16741 atgcgattaa agtttttggc gcgaatggct ctacgagcga tgaaattgtc atcgctgccc
    16801 tggaatacgc ggcggatccg acgggagatc tcagtttcaa ggaacaactc gatgtcgtaa
    16861 acctttcttt aggaagcggc tatggcaatc ctcacacgat gtacagctct gcggtgaaaa
    16921 acttgaccca tgggggcacc gtcgtggtgg cctctgcggg taactccggt aacaaaagtt
    16981 atgttacagg tgcccctgcg gtggtgaatg atactatttc cgtggcttcc agcatcgaca
    17041 atatggacca gaatattctc tttccgactg tgcagtttgc ctttgccaat gaaattttga
    17101 atgcagaggc tatggaagga gccgtcacca agcctttgtc tgaaatcagt tcactcaaag
    17161 gggaagtgat cgccgtaggt tatgctgaca aggattttga tcaggcgctt aaagatcaaa
    17221 tcaatggcaa agtcgccttg gttgatcgag gcagagtgac cttttcagag aaaatccaaa
    17281 gagcgcaaga ggccggcgct atcgcaatca ttgtggtgaa taatacagag ggagagccca
    17341 tctccatggg cggagaagga aaattcaata ttccaggggt gatgatcacc aaagctgctg
    17401 ggaccacgat taaatctcac ttagctgtaa cagtggattt aaaatccacc ctcaaggtcg
    17461 ataagccttg gctcgttgat actatttctg atttctcttc ccgggggcct cgttctgaag
    17521 atggcatgat caaaccggaa atcgcggcac caggctctaa cattatctct gctgaccgtg
    17581 gcggtggtga aaaaggcgtt tccatgtctg gaacttccat ggccggccct cacgtcacgg
    17641 gtgtcatgac tctgttgaaa caaaaataca aaaatttaaa cactcaagag ttgaagtccg
    17701 taattatggg gcacagcaag gtgatcgcgg acccagataa aaaaatctat tctgtcagtc
    17761 gtcagggagc aggtcgtgtg caaattgctg agtctctcaa tgcaaaagtg gtcacggttc
    17821 cagccgctct ctcttttggg atcacggata tcgaaaaaca aaaaactctc agcaaaaacg
    17881 tcaccataaa aaacatgagt acggaaactc tcactctgaa atctgaatgg agtggctcag
    17941 cgggcttggt attttccacg aacaatttga ccttagcccc cggcgaatca aaagacctca
    18001 ccgtcaccgt caaaatatta gcggcacaaa tgaaaagcgc caatgatgaa ttggatggat
    18061 ttttgaaact gacttcgact caagcaagcg gcgaagagac tctggtccaa atccccgcct
    18121 tagtggtggc tcgacagatt tctcaaatca gtgcaaagtc gttgctggtg catgcgacct
    18181 cgttagcgga tagcgcggga agcgtggcag agatcagcct gcaaaataca ggtctgaata
    18241 aagggagagc ttatctattt aatttgttag gcacggatgc ccgtaagaag gacgccaagc
    18301 cagaccccat tcacaatcac aattgcgata tgcaatcggc gggttacaga attatagaaa
    18361 aagacggcat ccgtattctg caagtcgcag tgaaactgta tgagcgcctg acaacctgga
    18421 acagctgtga agtcaacgtt cagattgatg gcaacaacga cactcaaaca gatcaagaaa
    18481 tcgcaggcgt gcctatgtcg agtcttccag ggttggccgg tgactctttc gtcagccttc
    18541 ttttagacgg caataaagcc caagacttgc gcaagcggtt tgaaacaaac attgcaaaca
    18601 atattaagga tgccaaagag gattacagcg aggccattct cgatcaaaga accatgcagg
    18661 ttgttgatgg atccacactt gccattatcg aggcaaatat ttctcagttg gccttagctg
    18721 acaccggcga gctgaatatt aaagtttcaa ccacgcatca agatacgggg gtcattgagt
    18781 acgatgattt cctcggcaaa caagaatcac agtggcaaaa aatttcggtc aatcccttgg
    18841 cacagtcttt tgctcaattg ccagaaacgg tggaactcaa aggccaggaa actcaaacgc
    18901 tgcacctgca aaaaggctat ggcaacggcg atttgatctt gtatgctcct cagaataggt
    18961 ccgtagtaga tgtcttgctg gaagacactc aatcacagac cgttcctgct agctttgaga
    19021 attaatgcat ctgaattatt accttgatgc actcactttt tttgtcacta aatgttttgt
    19081 aagcatccgg tccactatca atacgcactc ggtgcgtgat cacgaaagag ggatccagct
    19141 ctccggccac gatgcggtcg aggagtggct gcatgtatct ttgcgtgtga gtttggccca
    19201 ttttaaaagt taagcctttt gcaaaggccg caccgaaggg aattttatcc agcaccccgc
    19261 cgtaaacacc tggaacagaa actgtgccac cctttttaca ggcctgaatg gcctgtcgta
    19321 aggcgtcggg acgatcggtg gccaacatga ctgccgcttt gactcgatcc aaattggctg
    19381 tcacagtatg accgtgagct tccattccta cagcatcaat acaagcgtca ggtccccggc
    19441 ccccggtacg atattttatt tcgtcgagaa ttccctccac atcttcaaaa tcaataactt
    19501 cggcaccact ctcagccgcc atttgcaggc gcccttcata gtgatcaatg acaagaacac
    19561 gttccgcgcc taacagaaag gcactgcgga tcgccatttg tcccaccgct cccgctcccc
    19621 atatcgccac ggtatcaccc ggcttaatat ggcagttttc agcggccata tatcccgtcg
    19681 gtaagatgtc actgagaaat aaaacttttt cgtcgctaat gtctttaggg actattaaag
    19741 agttcgtatc tgcaaaaggg actcgaagaa attccgcctg acccccggca tagcctccaa
    19801 acatatgaga ataaccaaat agagctgatc ctgaatatcc atagagtttg tcagccatgt
    19861 gagaatttgg attggtgtta tcacaagccg aaaattcttg ctctagacaa aattgacagc
    19921 tcccacacgc gatatcaaag gggatcacga ctttatctcc gaccttgatt tttttaaccg
    19981 ccgagcccac ttccacaacc tcacccatgg tctcatggcc gaggatatcg cctttttcca
    20041 tggtaggaat atacccatca tacaaatgca aatcggaacc acaaatcgcc gccgtcgtca
    20101 ctttgataat cgcatcgtgc atatttaaaa gcgatgggtc tttgacattt tttaaggcta
    20161 catcatgctt gccttgccag caaagtgctc tcatttgtct tctcctcttt aatgggtcaa
    20221 acttgaaccg gatgttgagc tgggctgacc ttcgatggtc gggagctcgc ctgtttccaa
    20281 ataggcttta agcctcttta tatttttcat aattacaagt tcaggaggtt cgcccgtaaa
    20341 aagcaaagtc agctccgtga gctttccccc aggaagggaa tagtccatcg ccaggcgcac
    20401 gacagttcct cgatttgcgg gagccttttc gaaacggatc gtgccagccg tttccacttc
    20461 agaaccttcc acgcttctcc aggccaacaa ttgatcggga atctcctgga taatttctat
    20521 atcccattcc actgtcgcct catttttgag ttgcaggatc caatgagatc tatgctccga
    20581 caacacttgg acctgtataa gattttgtat aaataaggga aaatttttaa aatctcgaaa
    20641 gaaagtataa acttcggagg ggctttttct aatcgtgaca gcgtgccgac tcttaagctc
    20701 ttcatccggt tttttttcta ccttttccct caacggcggt tgaggttgaa cctcgtcttt
    20761 attagttgaa acaaatggat ccatttttgt ttccctcttt taaaagtttt gtgcattaga
    20821 aaaatatgca gtggagcccc ccaccttgtc tcttattttt tttgttttat gtatttataa
    20881 atagaaagct tgcagcttta taaataaaaa gcttgtagct ttataaataa aaagcttgta
    20941 gctttataac ttacattgtg agttgaatgg ggaaataaat aaacgagccg cacaaaaaaa
    21001 tgcattcaac cgccctctat tcacttttag tggcataatt gcgatcagta tttgacgatc
    21061 tcagccaatt tcggtacctc aatacaatga atattcaaaa aactttgacc acctctccca
    21121 agactttacc tcctgctgac aaattgggct ttggccaatt ttttaccgac catatgtttg
    21181 tggcgaaata tcgcgaaggt cagggttggt atgaagcaaa gattctaccc tacagtcctt
    21241 tgagtatgga tccgggggct tcggttttcc attatggtca agctttattt gaaggcatga
    21301 aagttttccg tcaagacgat ggcaaaattg tcttcttccg ccctgagttc aatcatcaac
    21361 gtttggctga cggtgccaaa cgtgtttgtc ttgaagctcc ccctctagaa ctttttatgc
    21421 aagccctcca agaattggtg gccgtggatg aaagatggat tccgaaggag cccaatactt
    21481 ctttgtatat tcgtccgacc ctgatcggaa ctgaagcgtt cctaggagtg cgaccttccc
    21541 atgaaaccac tttttttatt atactttcac ctgtgggttc ttattacagc gagggaaccc
    21601 agcctgttaa gatttggacc gaggaaaaat atctgcgtgc ggctccgggg ggattgggtg
    21661 ctgtgaaagc cggagcgaac tacgcttcca gccttaaagc cgcttatgaa agcaaacaaa
    21721 agggctatgc ccaagtcctg tggctcgata cagatcgcga aggcattgaa gaagtgggca
    21781 caatgaatgt gttctttgtc tttgataatg agattgtgac ccccgctctc aatggaagca
    21841 ttttagcggg cggaactcgc gaagcagtca tccatatttt aaaatcaaaa catcttccca
    21901 ttgttgaaag acgaatcact atcactgaag tgatagagaa aattcaaaat ggaaaactcc
    21961 gtgaagcttt cggtacggga acagcagcag tcatttctcc gatgggcatt ctggcttata
    22021 aaggcaaaga atgggttatc aatgacaaca aaaatggagc tttaagtacg gagctttaca
    22081 agcaaatcac ttccattcaa catgggactg ctcctgatat atttaactgg attgtccctc
    22141 tacaaaagaa tctctagaac cgatgaagct ttacacgaag ctttatagaa aagcggatta
    22201 tctcagtatg ccttggaaaa atggccttgg catgacagag caaattcaaa ttttcccaga
    22261 aaattgcctt ttctccgagg gccaagattt tctatggcgt atcagctcgg caaagattcg
    22321 aggggcctcc cccttttctc aatttccggg ctgcgatcgg tggctgatgg tggtgactgg
    22381 gcaaggcctc ctgctcgacg ggggtgccct cttgccacag gaaccccttc atttttccgg
    22441 agagccccca cttcattgcg ccttgctggg gggcgaagtc acggacctag gaatcattta
    22501 ccgccgcgat aaaatatccg cgatgatgtg cagagaagaa tttatggaca cttgttcact
    22561 ggatcttcca gcagggcttc attttattta ttgcttcgat gaacccctca aaattgccga
    22621 gcaaattctg gaaagcggtg actccctgga aatcgcaggc cccgcaaagg tgacattaga
    22681 ctcttccttt ggtggcaaag caaaatacgt tcgaattcat atccaggaaa aataagctct
    22741 cacttaaatt caattcagcg gacctcaact gcactcagtg agaatacagt ccgaaggagt
    22801 cctatttgtt agaatgcata aggattaagc tttacagcag gaccaatatg ctcttagcat
    22861 gcccaaagaa gcttataata attagtattt aacatcgcgg agcggtaggt tggcagttgt
    22921 tgccgacagg aatggatcca agcatgaccg ttcatcgcag tgatgcacat gcgacgcaag
    22981 tagacctatg ggcgagcaga aatattaaaa acctttctgc cgaagaacgc cctcagctct
    23041 atgcaaaagc aatccaggct attgaacggc gaagcctcaa caccttaagt agtgtcactg
    23101 ttttggtgat agttgatcgc gcaattcacg aaacaaaaga aaacttcccc ctcctcacgc
    23161 cgatcaaaag cgaagctgaa ggaattgatt ttagcgcact ttttgacaag cccgatatga
    23221 attatgaaga actggaaggc gcgcttcgag cacttctcat cgagcttctg aatgtgctcg
    23281 gaaacattac tgcggatgtt ctgaccgttc cgctgcataa agaactcatg gatgtaacca
    23341 ttgtaaaaaa gaagaatcgg gagcgaaaat gaaagacata cgggttgcaa caggagttcg
    23401 taacctcgac gaaatcctct gtgggggatt accgaatgga acattgacgg ttttcgccgg
    23461 cactcctggt tccggtaaaa caatcttgtc acaacagatt gcttttaaaa acgcttcccc
    23521 caagaggcgc gtgctcttct ttcaaacttt gtcagaaccg accgcaaaga cgttacgtta
    23581 tctgaaacaa ttctccttct tcgatcagaa aaagctcgat gacggcagtg tccagtttgt
    23641 tgatttaggc gatatcttga gaactgaagg tttggaaaaa gcactcagcc ttcttatgac
    23701 tcatgtgaag cgtgtaaagc ccttctttgt gattgtggac agttttaaaa tatttgaaga
    23761 tttggcgaaa tccagagaag agcttcgcaa gttctcttac gaggtagcga tcaatctcat
    23821 gggttgggaa tgtacgactt tactcctggg ggaatttagc cagaaagata ttgaaacgaa
    23881 tcctctattt tccatcattg atggaattgt ctcgctgaag cattatgccg aggctggaga
    23941 agaacaaagg ctcctgcaag tgataaaaat gcgcggcacc gatcacagtc gtgacgagca
    24001 tacttttacg atcaccccct caggtcttga gatttacgct cctcgcgtga caattcgtcg
    24061 catacctgga tcagacataa tgagtaagaa taatggtcct gcccgagcca agcttcgaat
    24121 ctcaaactta gatagtctcc tgggcgaagg aattccctat ggctccagct tgctcatctc
    24181 cggcgtcgct ggcacaggca aaacaatcct ttcgctggag ttcatctacc gaggtgcgaa
    24241 agattttgga gaaaaaggaa ttttcttctc tttcgaggaa acagccgagc gtctcataac
    24301 ctgcgcccgg aacatgggtt gggaaatcga cgatcaaatt aaaaaaggaa atattgaaat
    24361 tgtttacatt ccacagaccg agatccaggt cgaaaaacat ctgctcatga tgaaggaccg
    24421 cattgagctg atgaaggcaa agcgtgtggt cattgattct gcctccgtct ttgttcataa
    24481 aattaaggat cctcaggccg tgcgagaaaa aatattccaa cttggaactc tcgtgcaaat
    24541 ggctcaaggt atcggtcttt ttgtaactga cgttcactac ggcagtaata aaattagccg
    24601 ctttggggtc gaggaaaccg ttgtcgacgg catcattcta ctcacctcct cggaggtggg
    24661 cttgaggcgc gaacgctttc tggaaattta taaattacga aacacctctc actccgacgg
    24721 acgacataaa atgttgatcg agcaaggcgg agtgagacta gaaaaattct aaaaagagaa
    24781 tcagatgaca gattcaccct tgaacgacga aaagtccttt agcttaacca agaagctgcc
    24841 cacggtagag cataagcctc gggcccctga caacacgcta tcgtggcagg agcaagctta
    24901 tgaccgcctg aaagtactct atgaagtcgg caaattgctt tccacttttg aaaatgtcga
    24961 aaaaaccttt ccgaagattc ttgacttgtg ctccacttca tttccgttta ggaccgcagt
    25021 ccttattgag aagagaggct cgagcgttat gactgcgatg tggaatgtgg aaaccgtcac
    25081 cgccgagcaa atcgctcgag cgaccaccag ctcaaaagag tcttttattt atcttacagg
    25141 cgcttcaaag tgtgagtctg cgaatcttcg aaccactgag atgtcttcac gaacgctgtc
    25201 ccacgctcag actcgaacgc aatctgaaaa taaggacccc gagagttaca tcgtattgcc
    25261 tttatacgtg gatcaattgc cggcctttgg tgtcttacag ttggggggat cgtatcctct
    25321 ttgcgagaaa gatgttgaat tcgtcgatgc actttccaat cttgtcgcga tcgccattga
    25381 ccgatattat cggattcaat atgagcgcga gcttaaggcc aaagaagcgt tggaaagctc
    25441 tcacaaactc tctcgctcgg aagcttcagt cacggatctg gaaacagaac gggagcttcg
    25501 cgagaatttt gtttcgttac tcagtcatga tttgcgcact cccttatcgg ctgccaaaat
    25561 aagcgctcaa ttgattcaac gggaagtcgg catcacagag acaacccgat ctctagcatc
    25621 gcgaatcgtc ttcaatatga accgagtgga tcaaatgatc agtgatctct tagatgcaaa
    25681 tcgtattcgc agcggcgaga agctccccct gaatatcgag cccttcgatt tgatcgctct
    25741 agtgggaaga accttagaag agctcaccac aattcatggg gagcgtttta ttctcaaggc
    25801 acctgatatt cttgacggct attgggacca gagaagcatt cgcagaatta ttgaaaattt
    25861 gtgcaataat gccatcaagt atggttcacc ggacgcacca acaatcctga gtttaagtca
    25921 aaacacagaa aagattacag atcagattac agaccaggtc acaattgaag ttcagaatac
    25981 aggagatctc atttctccgg aagatcagaa aaccctcttt caacaattta ggcgaagcaa
    26041 gacagcccaa gacagtggca aaaagggttg gggcattggc ttgagtctgg ttcgtggagt
    26101 tgccgaagcg catggcggaa ctgtaaaggt tgccagtgat gcagaaagag gaacagtatt
    26161 caccgtgact ctacccatcg attctcgcac tcttatttca aaagaaggac catgaaaaaa
    26221 acaattcttg ccatagatga taataaagaa accctcttcc tcgaaggagt cgttttaact
    26281 caagccggtt atcaagttca cacggcctta ggtggcgagg ccgcattaaa aatgattcac
    26341 gagctcaaag aaatcgactt gatcttactg gattacgaaa tggcgggaat gaatgggcca
    26401 gagttcttag cggctttgga aaaacaatat cctcttgtgt tcgaacaaac tcccgtcatc
    26461 tatgctacag cccatgaaaa ccccccacaa aaattaaata aaagttggat ccctaaactg
    26521 agagacatcg ataagtttat taaggacgtc gaaaggttca ttactcccta atggggccat
    26581 gaaccctccg cagaacaaag gctctgagca cgggagccta aaacttgcgg cccacagtaa
    26641 accccgacac cttgctcgcg cagagtgaag ggcaaggcct ggtttcctaa ctgctcgcta
    26701 ctgagttgtt gaagatcact caggttcgcc atggcctctg cactggattt tatgttgagg
    26761 ttggcatgca tatgagtcaa cgggatcaac tccgccgctt ctgtgactaa gcgaaagccg
    26821 ttgatcctgt tttggtctgg tcgtttttca aaataaactc tcgagaacag gccgtagtta
    26881 ccacggacgc tctcgttatc tctactagcg gccccagtca taaagtaatt tcccaagctg
    26941 tagaaaatca actttccatt cacgttttca acaggtcgta ccacgtgagg gtgatggccg
    27001 ataataagat ccagatctcc gcggttcaaa tagtcatgat agcgcgctct ttgcccttta
    27061 tctaattcaa agactccttc cgtgccgtta tggatagaaa gaatttttag attggcattc
    27121 gagtgagcca tattttgcaa aagtttattc actagtgcct tgttatgcat cgctacgatt
    27181 ccagggttgt ttcttaaaat ggaaccttca tcgctcccgg tagaaactcc gccgaagcca
    27241 atggctgcaa acgcaatcgt aatgccctct atttcaataa taaggggctc cgtcgcttcg
    27301 tcaatgttcc ctgctaaccc tgcgtggtag aggcttttgc caggtcggcc gttgagcatg
    27361 ctctttcttt caatactttc cttgagctca gccagattac ggagggtatc cgcggcgcca
    27421 ctctctccaa aatcaaaaac atggttattc gcggtgctaa acaaattaaa tccataatcc
    27481 gctaaaatat caacgacctc cgggtgggtc ataaaagtaa atttcttatc atagtcagtt
    27541 aaattacgat cactactcac caccgtttcc aaattgccaa aattaatgtc gccagtgaga
    27601 agttttttaa aaacggcttg agcttgcaca gccaaacgca cgctgcgagg ctgttgtggg
    27661 aggcgggcca gcacgtcggg agcggaataa acaggacttt ccggatctct ttcgtcttta
    27721 gcaaggttga tgtctccccc catcgtaagg atgaagtccg ccttgaccac ttggcaggac
    27781 gaaaccaaca gcattaaaat tacaaatgaa tacatggagc ttcccgcctt taaaggcatt
    27841 gcatttgcga cattcatgcc atagcgaaaa gaggttttat ttaatttgat ggcaaaaaga
    27901 tgtctaaaac atctcacttt gggacgctga tgggccctgt tcaaagcctc tccgggactt
    27961 tgatcgtgac ttctgcaatg aagattctgt taagtccgtc cgctatttcc cgataagtgg
    28021 agcatgattt ctctttggtg tgtcatagga cttatcgccg caactatcag tgtttccact
    28081 ctgggagcca ctttttctgt agtgggtttg gccgcgcttt tttctggcgc ctctctggcg
    28141 gtgagtgcca tggccgcttc tttggaactc gcaaaattta tcctcgccgc ctaccttcac
    28201 caacgatgga cagagcttaa tattattttc aaagcttatt tggtggcggc aatcatcatt
    28261 ttaagtgcca tcacaagttt gggtatcttt ggctttttat caaatgctta tcaatccgct
    28321 tctgcggccc ttgaagcgga aagcgtaaag tctgagtctt taaaagctca gcaagcacat
    28381 aaccaagcgg aaatcagccg tcttactaga tccatcgatg aaattcccgc caatcgtatt
    28441 tctaaacgta ttgaagttcg caaagaaatt gaaccggcta ttgccgccct gactaaacaa
    28501 acacagcaac ttgaaagcct catgactcaa tctaatctgc gtacactgga gcttaaaaat
    28561 aaggtgggac cgcttatcta tatcgccaag gctttcaaac ttgatatcga cacggtcgtg
    28621 aaatatttga ttcttctatt ggtatcggtc tttgacccgc tggcgatttg cttggtcatt
    28681 gccaccagtg gtgccttaga gactcgccgt cgcggtgtcg ccgcttcgcc tccgccctct
    28741 ggtatcgagg atgaagttat ccaaatgcga tttgtcgacg ataaagatca aagcacagga
    28801 tagcccatgt ttctggtcaa agtgctcgaa agaaaaggcg tcatccggtt ttttggtttc
    28861 ctcctgcttc tttctccttt ttttaatact tttgcaaaaa tgtccctgct gacagaactg
    28921 tccaatcggt ggagttttaa agttttttgg cagattctca tgatgggctc tctgtttaat
    28981 caaattctct atgtggccac ccttatcatt ggcattctta tgctccgagg ttccgtcaag
    29041 gcttggaact tcgcactggt cctcctcgca ggctatatcg ctcttcaagt ctctgatttt
    29101 ggccatgtca gggccagcaa agtgacatgg ttgtttttta tcaccaacat ttttgttttt
    29161 ttatttatcg ccgaccagct tgtgtggaaa gtaaaagcca ctaaaccagc accgacaaag
    29221 gagaaggtcc ctgctcctct gattcataaa aagatcatta tccatttcgc cggcatgggc
    29281 ccttgggcgc aggttttaag tatttccaat aaaggcattc acgtgcgaag cctcaaagct
    29341 ccgccgttag atattacaag tcgagaactt gaaatctcat tgaagaatgg gctcaccttg
    29401 cgcacacgct ttgcaagtaa ttcgaatcaa gattactttt ttaattatat aagcctcagt
    29461 cctgaagaaa taaactcact caaccaatgg attctctcca tatcttagtt ttcacatagg
    29521 aagcttatcc gcgattacat ctattttttc gatcacgggt tcttttgcaa gcaaatccag
    29581 atcctttgcg aataacgcct ccgccacttt gccagaaaga tgggcccgac gtcctgactc
    29641 atcaggaaaa gcatcgaaga ttccgaactc tcgttgactc aggcgaaggg cgaaccacgc
    29701 ggtggtcccc tgctcttcct ccacgagcgc acgtccactt cgtaaaaatt cttcgacttc
    29761 gccttctttg cccggctttg cttctagacg catgaacaat gcacattgaa ccatattggt
    29821 ctccttgaaa aaaatattgg acacctctca gtaaagtaat ataccggcga actgccaact
    29881 ccggagaacg aatagtgtta actctcaatg aattcatatt cttcgtaatt tccgtaaatt
    29941 ttcttccgat agtttagata gcgagctcct tgcaatttcc agcatacttg gcactggagg
    30001 ttttttatga atgcaattta ttttaaagat aagtcaggca tgggttcctc tcgggtgatt
    30061 cttttaggtt ccatctatct gctattaggt atcttcgcat tggcctttgc ttcagcgact
    30121 acgctagccg ctatcattac tttaggcctg gtattgatgt tcgtgggaat tgcagaaatt
    30181 gtctatggcc tccaaggacg caagcgaggt caactctggc cccatgtatg caaccaatgg
    30241 tgttctctcc gttattctcg gaggtttgat cttaagatct tttcccatct ctgccttctg
    30301 gactattggt gttttcgtcg gcattgatct ccttgtgtct ggagcttcac tgattggctt
    30361 gggagtcacc gcaaaaaaag ccagaaaaga actcgtgggt caggtctatt ccactctcca
    30421 tcccgaaccc ggctctcgca cagaactcga tagagaagaa catcctttgc actaaagtgg
    30481 atatttaaaa tattgagaca aacaaggacc aagagccaga aaaccctgac ctaggtcaag
    30541 gacttgcttt gtttttaccc tgatactgtc tacaatggac gcatgcaaaa acatatccgt
    30601 ctttttatct tcgtcgttct atctttaaac ttttccttta ctgcggccag ggccgcggtt
    30661 tcaccaattt cggtggctat cctaccgccc gtgcagtttc cgccagggga tttctctgtc
    30721 accgggatcc gggccagcgc tctctggggc aaacaccgtg acctctatgg cttggatttc
    30781 ggtctgttag gaaacattac agaacagagc tttaccggtc tttcggcgtc agggattttc
    30841 aacaacaccc gtggtgtcac aaatattctc ggtcttcaat ttgcaggagc tacgaatatc
    30901 aataccaata aagtcagcgt ttatggactt caagccaccc taggtgtgaa ttacaattct
    30961 gcggcttcaa cagtcactgg agttcaactg gctctcttag ccaatgtctc ggcctttaca
    31021 gaaatctatg gacttcaggt gggtctctac aatcgtgcga aagatgttta tggcttccaa
    31081 attggtctag tgaatgtggc ggacaatctt catggtgtgc aaatcggtct ggtgaatttc
    31141 aatcataaag gtccttttgc catctctccg attctgaaca tcggtttcta acttcgcttt
    31201 taggtgaaat caaaaagaag aagggcaagg aatcgcttct ttgcccttct atttattttc
    31261 aaataaactt tcaatattac cgcgaattaa aacccgtcag tcgtccccac tttgcgagcg
    31321 gaagaaactt catcttcatc aaacggaatc atctccgacg aagaagtcac cgcctttttc
    31381 ttggcacttg ccgcaggctt tttaaaattc aaaacctttg ctgacggaga cgatgacttg
    31441 cttttatgag ccggagcttt cgcatacgaa ctttcctgct gtggcgcctt ttgtgctgag
    31501 ccatagacca aggcctcaag cgccagcaca cttgctctga gactttcaga ttgagaagac
    31561 aattcctcag cagcggcggc agtctcttcc gccgcagcgg cattttgttg agtgacctca
    31621 tccatttggt tcatcgcttt accgatttgc acgatgccgt tgcgctgttc ttcactggca
    31681 ttggcgattt cgttatttaa gtcagcgact ttctttaccg ccgtgacgat ttcagcgaga
    31741 accgcgcctc cctgattggc ttgacggctt cccacctcaa ttttttccac gctattttta
    31801 atcatttccg caatttcttt cgctgcggat gcacttcttt gcgcaagatt tctcaccgct
    31861 tcagccacta cagcgaagcc ctttccttgc tcaccggcac gggccgcctc caccgccgca
    31921 ttcaaggcta agagattggt ctgaaaggcg atatcatcaa tcacattcgt gatctcttca
    31981 attttcttag agtctgcgga aatcgtatga atagagtcaa tgagagtctt aatctccatc
    32041 tcacccttaa gcgcaatttc tctggtagag gctgctaacg aagccgcttg tttcccgttt
    32101 tccgtattca gaccaaccat ggaggttaac tcttccatcg tcgccacggt ttcttctaga
    32161 gaagaggctt gttcggtagc ggcctctgaa aggctctgtg aagatcccgc gatttgattt
    32221 gaggcctgag ccacttgatc tgagccaaga gctaaatctt tggaaacagc accgacggca
    32281 cgagtgatac tcatggcgat aaagaagcca atgcctaaac ccaagccaag acccgctaga
    32341 atcactagaa gactaatttg attccctgtt ttttccgccg cttttgcctc cgccacttta
    32401 gcctctaaga ctttttgatg aaactgaatc agttcttcag ccgcagccgt aaaaatcttt
    32461 gctttttctg gacaatcttt aaggaaaatc tgcaacattt tttcatgatc ttcaggcttc
    32521 ccagatttat gaaggctgat gacttgctca ccagtcgctt taaatgattc ccagctagaa
    32581 tgaagcttat cgaaaacttc tttttgtcca ggaataaaac caagagccac gtattcctta
    32641 ctgaattttt cgtattcttc aatcgtattc aaggcaccct taattgtctc ttcggaatcc
    32701 gccttactca gaccctgaag ccccaaggtt ctcaccataa tacgcacgcg acggtaggcc
    32761 agcatcattt catagcttag ctttgtcttt ggaagacttt tgtccaccac aaattcatag
    32821 ttcccagtta cggaactcat ggactggtac gaaacacccc cgacgatcac actgatgctc
    32881 aataaaaacg cacacagcat gagtaatttc acattcaggc tcagattttt cttcatattt
    32941 tcatactcct tttgagtcaa acaacccatg cgatcaggaa ccccttcctg atccgtttcc
    33001 tttttcggca gatcttgaca atttatgaga ctggttaaag aaatttgaca ggtttctgcg
    33061 gccagataga atatatcttc actggatgga tttgatacga ttatttatag caagcataga
    33121 tattgtggcc acactttcct cgatcatcaa atgttgaaac aaggtatgct gtatatccgg
    33181 ccggacaact aaactggtca ttattgagat gattcccttt gcagcgtccc caacttacac
    33241 aattacaggc gtcatatgtt cctccaaaac ccacatcacc tacgccgcat ccgagaattg
    33301 tatcactggc attggtcaat actgtggttt taaccgtgat cggtctaagg ggtctcacca
    33361 aaccgtcact ttcgaattca accttaatat tggccgagta taaatttgta tctattttta
    33421 caaagtcggt gatcgtaatt ccgccacctt tcactttcaa gttcttggtg aagggtgagg
    33481 ggtgatctcc aggccctatc aaaatttttg ttgaatccat cgcatttgaa tgaaggccat
    33541 ttgcaagcgt aaacgttgtg gtttccggct gcccagcatt gactttaaaa ctgttatttc
    33601 cattaataac agagggagtg agctcagcgg tacagatctt cccatccgcg agagctgaga
    33661 ttaaaaactt ttcaagatcc aaagagccaa gcttctctgt caatgctctg gtttccttct
    33721 gcatactcgc ctgcattgtg agcataccca tcatggcaat tgataaaata ccaattgcaa
    33781 tcatcacttc gattatgccc atacctcgac tattaaaagc cattttcatc actccccttt
    33841 cttaagtcta gtctcaagga gaggccttta aaagcagtat aaagatcatc tgagatatat
    33901 aagacacctg aagggtttag accagcagca gtgcctcata gagatacact tctctcaata
    33961 attgttgaaa attttgtcaa atttcaaaac ttttggtaat ttaatcgaac acacacgtct
    34021 gctggttttt cttataatga acttcaggaa ggggaccact atgaggcaga cgattgcccg
    34081 agctattttc tctttatcat ttcttctttg catgatttta aatatttctt gttcaggctc
    34141 tggttccggt tatggcagct cttcttctta ttccaggacc gcctccgcct caaccatcac
    34201 cgccgctaga gctgcgccag gttccgcgca ggaggcctta gccgatagag tgcaaaaact
    34261 tttttccgct aaatgcctga gctgccacaa cgcgcagaag gcttcgggca atatcaatct
    34321 ttccactgca accgcaattc ttcaacgggg ccttgtgatc aagggcaatg ctgcgggttc
    34381 gcaactttat cagtccctcg tcgccaatcg catgcccgtc ggaggaggcc ttgatgacgc
    34441 cagcaaaaaa gatgttcagg attggatcaa tcagggaccc tttgaattcc ccagcacatt
    34501 caatggctcg ctttcagtct ctaagcaaga aatccgtccg gcaatttctt cttcctcttt
    34561 ttttggcgat tgggagtctg gcacgctcac tgggagtggc aattacaact ggagccaaag
    34621 tcaaatttcc acaaagagca gctacacttt gctcaacgat ggaggaggac gccagggctc
    34681 ctattatgcc cgggtctcgg tcgcaccgag gcgatgcaga agttgcgttg aacgctctga
    34741 agttgtgcaa atgcaggatg cgcaaaacca ccctatcgtt gaaaatgaaa gcagtgaaat
    34801 ccaacgctac tccttcagcg taaaatttga ttcctcttgg aaacccatcg tcggcaacag
    34861 aagcgaagcc tggggcatct ttttgcaact tcgcagtccg aacacttttc ccactcgcgc
    34921 cgcctgggca ttcaacgcct caaatacaat tacttttagg caaagaggcg gtaacttaag
    34981 gtacaataag ggtaaaatat atcctctttc aaacaactct ctgaatacgg gaaaatggat
    35041 tgatttcgtt cttaccataa agtacgcgaa agacaatacg ggcttcttcc gtattcaccg
    35101 ccgagacgaa ggagaaacga ccttcaccga agtcttaaat gtaaccgagg tgcctaccct
    35161 gcaaactcga gacggagtgg tcgccgatca cgtcataagg catggtctca gccgaaatca
    35221 ccacaatttc actagtattt tgtacctcga tggctttacc cgagaaactg taagcccaac
    35281 gcctgccccc atcccaactc caaacccaag tccgacacca actcctcccc ctgcaaccaa
    35341 tgcccttgca gatcgggtgc agaaaattct aatcgcaaaa tgcgtgaact gccacaatgt
    35401 tcagatgcct tcgggaaatg tcagtaactt gaccagtgct cgagttcttt tagaaaaagg
    35461 actcgtgact aaaggcaacg caaccacttc taggctttat caagtggtgg ccaccaatca
    35521 aatgccactg ggtatcgctc tggatgaggc cagtaaaaaa gatatagctg actggatcaa
    35581 tgccggcgct cctgaattta cctcgaattt gcctcaacca acgcccactc ctcctcttcc
    35641 cgacccaacc cgcataccac aatcagtatt ggatcttgca gcagatgaag agaccatcta
    35701 tcaggatctc cagcagatcg ccgccgaaac acgagccttt actcgctata tcgtcatcac
    35761 gcaaaactat aattccaccg gcattttgac ggcacaaaaa gctcttttcg ctaaaggcat
    35821 cagcaaaact ttgaacagtc ttcactggaa gaacacaata agtattcctg tggccttaaa
    35881 ccctgaaagc acggctttgc gcattgattt gcgcaactat gatctcactg cggcagaatg
    35941 gtcacaaatc acggcgacct atccctatct gaaccaactc aaagcttcga gtcgctggaa
    36001 cgccatcgtt tccgcgacaa aaagcactaa gcccattgtg cgcggtgatt ggttcgtgca
    36061 acaggtctta cagcccaccc tttacaaaca actgctggag atccccgaaa atattcaaac
    36121 gctcgaaacc cggttaggcg tgaacgctga ggaaagactc cgcagtggcg ccgccattcg
    36181 agccggcctt aaggattcag gcgtgtcttt ttcaaatcgc atcgctgaac gctatgaaag
    36241 ttcttacggt gcttactgga aaagttatga ctttatttcc agcgatggaa accaaaatat
    36301 tttcagaaac cccttgggtc ctgtcttgga taacttcagt caaggtatcg gcttcgttca
    36361 taatggggga gaaatgatct tcactcttcc aaatggcctg catggttatg ccttaaccag
    36421 tgccactggc aactacctca acaccgcacc ccctgaaatc gttatcgata ctagccgcgc
    36481 agacaaatta gttacaaacg gaagttcctg ctttacctgt cacactcagg gtttgattcc
    36541 cttcgaggat gaaatcagcg ccgcttcaaa cagaggccat cttttcaaca acaagcaact
    36601 gactgcgatc aatcgtcttt acattggtaa aaccaaactt gaagagacct ttaacaaaga
    36661 tatggcggct tatgtggacg ccctggataa actgcaaatt tcccctgact caggagaacc
    36721 cgtttcaacg gtgaatttat tttatgctcg caatcttcaa attaaagata ttgctgccga
    36781 gctgcagctc agcgtcactg atctgagaaa atatttagaa agcagctccg gcctgcgcag
    36841 tcaatttgat ttcactaaca gccaccgcgt gagcactgtg aaaatcaccc gggctctttt
    36901 tgaacagcgc ttcaatcaaa ttttacagga ggtggcaaaa ctaaaaccct aactcatcct
    36961 tatttttgca aagctttttc tttctgtaaa atatctttaa cgattcgtat cgcggacata
    37021 gcggcaccat ctgaatgggt gccctccgtg aaatccccac caaagtagac tcttccttgg
    37081 ggctttcgaa gcgaatcaga gagatcatca aagcgagaac gccctacagg ccaagaggcg
    37141 atggcacgtg gatgataacg ataaaaaact attttcttta tatgcttacc aatcccaggc
    37201 cataatttct caaagtcctt caaaaggaca gcacggacat catccagggt ccctactctg
    37261 gcattgaatc cttcagcggg gtcaccagta atcagcaaat tcagcatcgt attcttttta
    37321 tcgccctcac tttttccctc ataaatgact cctaatgaac tgtcggtgag gatcggaaga
    37381 atactttccc ccttcacagt ccaaaaatga gtggcttttt catctaaaac caagtgcgcg
    37441 gtaaaatagg caccccagcc ttgggattga atcgcttgaa ctctctcagt ggcaatggca
    37501 ggttcaaact gaatttcatt cagacgaaac aagggcggag tcgtaaccac atacttccca
    37561 tgataggtgt gctgtttgaa atccgaatcc gtcgctataa cctccacccc atcggtggag
    37621 gacttgatat tggtcacttg tctatttagg agtatatgct cttttccaat agaatccgcc
    37681 attgccaaag ccgctttatg attgccgccg atcacatgaa aagacgatga tccggccccc
    37741 gaaaaaatat gccactccgc gattccgtcc atggcactga tccggctcca gggagtccca
    37801 tactccggct cgctttcaag gcggatgagc tcttggactt ttttagaaag cttacaggtc
    37861 ttctgaatcc agtccgcaaa agagagatct ttaagaaaaa gaaccgactt gtccttagga
    37921 ttggataata attttttata aatatcagct gtttttttat cccagttttc atactctttc
    37981 atttctttgg cgttaaaaaa agaggctaag aattccagat tggtattttg cgtaaaaggg
    38041 tgaagtttgc ctttatatat aaaactggaa aaagacgtgg cggacttttc aaggggaatt
    38101 ttcaattctt tgaaaatttc caaactggga tttccttccc aaaactcttc caatcccact
    38161 tccgccgaaa catcttcggt ataggatgcg gttctgattc gcccgccgat atgggaactc
    38221 atttctaaaa caagaaaagt cttcccctct tttttgagcc gataggcggt gctcaaacca
    38281 gcgattccgg cacccacgat gaggacatca ggctccgttt ttttaactgc ttcccccgcg
    38341 tgaaccaacg aagtggcggt taaaagaaca ataatcataa gtttagggaa gcctttaaac
    38401 gaattttcta accactttga ctttaaaggt ctagaattga atggtcgcat atttattttc
    38461 ctcttaatta tttatttttt tctttaacca cttgccagcc atccgtcttt tctaatttca
    38521 tcggataggc gtaggagctt cgtgtttcca tcaaatccat ttgctgaaga atcagcttta
    38581 aggcgtcggc ggcattgaat cgagcctctt tcttttcatc gaatttttcg atgatttcta
    38641 ttaaagccgt gcgggcttgc accggaatag ccgaaacttc aacgagaacc tgtgcccgct
    38701 ccccgttaat ttccactttt tgcgctttaa attcggacct gtcggtgaga actcgcgctg
    38761 ccgtagcttg ggtttgaatc ttccctttca ccccttttgc aatctccgcc ttcatctctg
    38821 tttgaaattg ttcccttgcg ccaccaatgg ccgcctctct gactttggat tcttcagaac
    38881 aggcacacag gaggaacagc caactcaggg ctattaaatt gagggcgctt aatttttttc
    38941 tcatgtaaaa atattagcga acggagccta gaacatcaag acttgaacgc aaagctggac
    39001 gaatccgctt ttgaattttt ttatattttt tttgttcgtc ctttttgacc tttttcggcc
    39061 gcccgaagta gtcactaaga accgttaaat catgacattg tccaagactc tcttgaagat
    39121 cttttaaatc gtttacgggt agatctaaaa actcaaaagt ataacgaatt tttttcatcg
    39181 aaatacgtaa gagatgaatt tctttttttg aaaatttttt ttgccgtatc cattgtttga
    39241 gttcagtgct tactttttgc aggagtttgt ctcgcacagg cactctatcg acacccaggc
    39301 cttccccaaa aatgcgtaat tcatggggga ttttttgaat ctctgggcgc tgcaaaattt
    39361 tatgtaattt acttccggcg gccctttgat ctttcagaag gccttgtgta ttcagatgat
    39421 atctcctagc tccctgcaaa gccacatccc attggcgacg atcccccaaa agatctccca
    39481 gctctttcaa ggattttttt gccgccttgg gcaccttatg ggccgtgctt ttttcaagca
    39541 aatcaatcaa ggatcgcaat cttcgcgtgg aaactcgaag atcatgaacc ccttctaaac
    39601 tgaggcgctg ggattgcgaa tgcatgagct tattaaaaat ctttacctgc tcttgaaact
    39661 gattctccac gacttccata actcctcaaa ttttcttcac tcccagctct ttcaaggccg
    39721 atgaaagagt taacattgta accttctggc tcaagctatc gatccacgct ttatggcctt
    39781 tgaggctttt tgtaaccacc ttaaggactt ccagccgggc ataatcctta tcattcgctg
    39841 ggatcaagtc ccaagtccca cccttataag aggttctttt gaacatttca tcagccgccc
    39901 ttacatactc cgtccactga ctttgcgctt tcacatcatc cggggtgatt ttccattgct
    39961 tgtagggatc tttaagtcgc tcttcaaaac gcttcagttg ctcatcttta tcaatagcca
    40021 gaaaaatttt gatgaaatca actccatccg cgatgagcat cttctcgaat tcacagattt
    40081 ctccataggc gtcctcaaga cgagttttcg gagcgagcct attcactttc tcggtgagca
    40141 ctcgcccata ccaagatcta tcgaaaatag caatgtggcc gggcgacggc aaacgagtcc
    40201 aaaagcgata caggtagtgc ttcccttgtt catctccttc cggaggaccg atcgcatgaa
    40261 ctcgaaaacc ccggggatcc agggactcca ccaagcgacg aatagcgccc cccttccctg
    40321 ccgcatcaaa accctcaaac aaaataatgg cgcgtttctt ttgatgccaa atcccttgtt
    40381 gaatacgcag aattttaagc tgcaggtctt taagatgctg gtccgtttcc ttagaccctc
    40441 tgagatgagg aaaatccctc ttaagcaaga gttttgagag tttcccagaa tgtgacaaaa
    40501 tcttcctcct gtttggcaat ttccgattta tagactcacc gtcgactcca tgtggtgcca
    40561 gataagttgg gataaatcgt gatccgagac atgcacgagg tctttaggca aagtactact
    40621 caccgtttcg aaagtttttt tcgaaagagc cgctctaatt tttcctaaaa aatggggttc
    40681 cgccactaaa atcaaatttt gaaagagatg ggcattaaaa cccctttcaa gctcttccga
    40741 aacatcctta gcgaaaatct gcgccacccg atccgtgggc ttctgcggct tcgtcaaact
    40801 tgtttgtgaa taggcgaaac taaacttatt gactccggga cggtcggcat tgatttcact
    40861 atttttgaga cgcccccggg ggtttgccac ctttttaaga agcttcaact tattcttttc
    40921 tttttcattg atttcgaaaa atctagcctc agtcctattc atcaccgcca cccaagtttt
    40981 catggtccct ttcatggtcc ctccgctgag aacattttat tttattattc tatcttcgtt
    41041 ttgcactcca aacacttcac gttttcggaa tctttaccgg cgatggagga actgagccaa
    41101 tcacttagaa tcgtaccatc ggatttcgtg gaataaaagc tggcattatt tactgaacaa
    41161 tgagtgattc ccggggctat aaaataatgg aaacgtccta ccaaactgga tgtctcgccc
    41221 atgtttttaa gcatacggga cgtccactcc agcccatttc cgcccatcgc cgcataatat
    41281 aaagtctgaa tacgatcttc ctcgtgatta aattgcgaaa agcgtgcctg tggataatag
    41341 tcagccaccg ctttataaat atcgacaatg tttaatttgg accattggac gctccctgga
    41401 tttagggcgg gaatccaagg cggtaaagct ttttcaactc cccattgagg aaagaataaa
    41461 gaaggagtca cacccgcgcc ggaatcccca aattgcaata ttctggcctg agtataattc
    41521 tcggcaatgg tcggagtcca caccaccgag ccataggatc ccgcactgca accatccaca
    41581 ttgatttccg ctgcggtttt ataattctcc atcatccatt tgagaacagc cttggtattt
    41641 atagctcctc gatgatggat cacataactg ctgttatctg gacgagtgta ggtggcatca
    41701 ctggtcccca gatgaacgtc ccctgtgcaa tagggaataa aaatgtgggt ccagctttta
    41761 taaggatttt ttttattttg aaaatcaaaa attcccgcct gccgattcac ttgcccatag
    41821 gttttcgcaa cggtgtccgt atagatcttg gtgctatcgc aagtaaagtc atcccagcag
    41881 gctccacccg cggcgaaact cacgacgatt ttctggggat cgcctttacg aacgaggaac
    41941 gcgaactcgc cgccacgggc acacaaagtc tctcctccag gttttatctc ttgccaaccg
    42001 gacggtaaag cgtaactcaa atgagatgcc aataaaatga taagaaaaag tttcatatcc
    42061 cctccatagg taccctgtgc actatatgaa gatccaggcc aattcgcaat gttcccagga
    42121 aaaaaacaaa ggccccctgg agaaaggcct tcactgatga ctcacaaata ccgaagaata
    42181 tatgtctttt atattctatg gaggtacgct tgaaacaatg ggtaaagaag ctcgttgatc
    42241 aattggattg gaaagaaacc gacgccagca ctcccagtcc gcatcaagat ctgacggaag
    42301 acagggccac tctgctctat atactggata attacagtaa gcatcttttt gaaattgata
    42361 aacaacctct gcgacgtgtt cgcgagacct ttgattcttt tgtcaaaggc cttgtcagtc
    42421 ctgatcccaa gtcagaaaaa ctgctctttc aacttcgtca attcttctca agttatcgca
    42481 ttgatgaata cacctatatt caaaatacct ttgatgattt caaacgtatc atttgggact
    42541 tcgctgaaca actcagtgag gatattgagt acgaaaacgc caaggatgcg gagacctccc
    42601 aaagcctcga ggaattgcgt ggcgccgtgg atgcaaactc catcgaggcc ctgcgctcga
    42661 aatcccgtga gtttatcgat tactatatca aataccaggc gcaaaaaaac gaacgcagaa
    42721 gcaaacgtct gaccacgatg aaaaaaaatc tttcctcaat taaaaaacaa ttgatggagg
    42781 ccaaccaaaa catgcgcgtt gaccatctga cgtccgcctt caaccgcaag agctttgatg
    42841 aacaaatgaa aaaacatctg caaatggcgg cgatttcgca aacacccgtc acggtgatga
    42901 ttctcgatat cgattttttc aaaaagatca acgacaccta cggacacgat atcggcgact
    42961 acattctaaa agaatgcgtg aaaatgctga aagaggtctt tcctcgcgct gaggacttcg
    43021 tctcgcgtat cgggggcgaa gagttcgccg tgattctgcc taactttaca gctgaacacg
    43081 cgattgtaag agccgaagag acgctccaga aaatccgcaa agaggttttc gtccatggag
    43141 aactgaagat ctgttttaca gtctccatag gaattgccca atggcttcat aatgagacac
    43201 tcgatcagtg gatcaagcgt gcggactcag ctctctatca ttccaaacaa actggccgca
    43261 acaaatatac agtggcccca cccgaaaggg gcatcaaaca agtcgcctga gtccgtgagg
    43321 tttttaacac cctgtctagg cactggtgag tgcctaccaa agcctagaca gttcgtgttt
    43381 tctaacagtt ctccgcgcct ttatttcttc ataatgttgg tctttgcttt gcaatagcgt
    43441 caggcatgtc acgcatgaag acctttctcc tctgggctct tttcatagcg cccttaacag
    43501 ggcccttttc cgcaaaagct gatgtcaaca tgaaggatgc cagctacttc aaaacatgga
    43561 ttgatcttga aatacggaag gacactcata tctttcaact gcggcgaacc tacaacagcc
    43621 gctccctgca tccgggactc tttgggtttg ggtggtgctc cgactttgaa aaaagcttgg
    43681 acctcaaccg cagcgatcaa atacttctgc aggattgccg cctgaatgcc ccgataagat
    43741 tcaaaaaaat aaaagaaaac ttgtacgaat cgccgaataa agaaaccctc gagcttaaag
    43801 aaaatcacta cctcagaacc accacggaaa aaacttttca aaaatacaat aaactcggaa
    43861 agctcctcag cctcatcgat accgccgggg atcatctgga tctcagttat agcggaaatg
    43921 gatttttaca aaaaattatt tttaaggaca tcgaacaaaa tataaatttc gatcttaacc
    43981 atcgacatat ccaagtcatc aaatcaaaaa atggccagga aatcagctat cgctacgaaa
    44041 acaataattt aacgcaagtg aacaactctt ggaaaaatat ttttctctac agctacgatg
    44101 agtttcacaa tctgacccgg attcaagctc cccaaggagg tcaggaagtg ctcacttacg
    44161 atgcggaaaa agactggata cttcgtattg acagcgcgaa tggaactctt aataaatgcg
    44221 ctgaaatctt ccattattat cagaaaaagg acatgagtta cgtctcgacc gcggaaaagc
    44281 ggtgcgcagg aaaaagtatc gctaaaacaa tctatgaatt ccggtacagg aaacgggccg
    44341 atggatcaaa atatctggag caagtgaatg tcacacaggg aaacaaaatc cagagaattt
    44401 cttatcagaa taaaccagga cttctcaatg aatctccata aattaaaaat tctgtttttt
    44461 actctcttct ctttttccag cctcggattc gcctctcccc ctgaagggct gcaaatggcc
    44521 gaggctcggc gcgcaaggcc tctcaatcaa ggcagcaaag atattgtggt cgccatcatt
    44581 gacaccggga tggacacttt acatcccagc cttcaaggaa atctctggac gaacttcgga
    44641 gaaactggaa tcgatagcca cggaaaagat aagtctcaga accagattga cgatgacggg
    44701 aatggcttca ttgacgacgt tcatggctgg aactttgccg acaacagcaa tgatctttcc
    44761 gacctccacg gtcacggcac tcatgtagcg ggcatcatcc acagcattgc tccaaaaaca
    44821 aatattatga tactgaagta ttatgatgca aaagccagtg gcgcggaaaa tctcaccaac
    44881 accgtaaaag ccatccgcta tgcgactcaa atgaaagcga aaattattaa ttattccgga
    44941 ggcggaacca ataaatacgc ggacgaagag gtcgccattc gcaaagccca attgcagggc
    45001 atcctttttg tagcagccgc aggaaatgag aaaagcaatt ccgatatttc cggtttttat
    45061 ccggcgaact acggactctc caacatcatc tcagtcaccg ccattgacga gagcggcaaa
    45121 gttcttcctt caagcaacta cggcagtcac agcgtggata ttgcagcccc cggaaaacgc
    45181 attgtctcca ctcttcctgg aggaaaattc ggagccatga ccggcacctc tcaagccacg
    45241 gcttttgtga gtgggactgc ggctttgctt ttggctgaag agaaaaacag gaaagcgcct
    45301 gaaagactga ttcaacaact cacgcaatct ggcaatacca atgacttcct tatcgataaa
    45361 accaaacacc agaccacgct caacatttac cgctcgttaa ttatgaaaga ctccaacctc
    45421 ggagcctttg gcaatgttga cgaaaccggc tggtactctt ccacggattt ctcttcaaaa
    45481 ttaaatgaga actccgaaga cttcggcagt gccgaactcc cctttaaaat tcaggtgaac
    45541 aggaaataac cttcactcct cgagctccgg aatccgcgaa aaagcaatcg ccgtaggagc
    45601 cacatccttt ttatcttgaa ggcaggccag ataaacagtg tctttgccga atttctcatt
    45661 gatcttatcg acaacgccaa agagccgctc acgcctggta ttttcaaaga aagaaaactg
    45721 atgattctcg gacttgataa aatctgataa aaccaccgag accatcagcg gcttcgcagt
    45781 ccggcaattc ccagacatct ccgcaaaaaa gcgatgcaaa tgagacaaca acaaagtcgt
    45841 gtcctgagtc tcttgaagct tggcagatcg ctcaaacttt tcaaattggt ttatgaattt
    45901 aactgacagg ctcagacgtc ttgctagaaa attctctttg cgtaagcgca cggcggtttt
    45961 tgaaagaagc ttttgtgcca ctcctaaagc tccttcccaa gatcttacat ttggcggcaa
    46021 aacgtgttga tgactgatac tctgggattt gtttctggga ataagaatgt tctccccgcg
    46081 caagagataa taataacgac tgcccaccag acttccccac agcccgtgca tttgtttttc
    46141 attgagtccc agcatctgct gcatggaggt gattcctttt tgatttattc tctgctccat
    46201 ctttggcccc acgccgggaa tgtcccgcag cttcaagggg aacagtcgct gaggcaactc
    46261 ttcaggctca atcgtaataa agccatcggg tttttgcata tccgtggcca tttttgctaa
    46321 aagcatgttg ggcgccaaac cgatcgaaga cgtcatgcaa acgccgacac gctcgcggat
    46381 ttcttttttt attttcatcg ctaaggtggt ggccaccgca agatcctgct gagaccccgt
    46441 caactcacag gcgaactcat caatcgagca tacggaatgg atgggaacgc aactctctac
    46501 gacatcccgt attttttgat gataaccaac ataagtttta tgtctggctt gcactaactt
    46561 gagaccggga cacatcctct tggcttcgcc tactaaagtt ccggttttaa tgcccttagc
    46621 tttggcttca tagctggcgg ctaaaaccgc cgttgtattg gccatcattg gaaccacggc
    46681 cacaggcttg ccacgaagct cgggactttc ctgctgttcg caggaagcaa agaaagaatt
    46741 aagatcaaga tacaaccaac gaaccattgc tggaagttaa cacaaaggtt cggcgacctt
    46801 gtagattttt aaataattcc ggcctgacat agtgcattcc taaaaggtac ggcgtacctt
    46861 ctagcgctta ttgataccta cgaggaaaat ttctttgctg atttttctgg tgctgtctgg
    46921 cttcacggct tcgaatcttt ggaaatcttt tttgatttct tcgcgaagct tggcgaactc
    46981 gtcactatga aaaagcttgc aaacgaagtg gccaccaggc tttaaaaatc ttcgcgcgac
    47041 atcgagagcc agttcgcaaa gggccatgga gcgtgcttga tccgtgaaac gatgaccggt
    47101 ggtattcgga gccatatccg acatcacaat atcaaaggga gggtggaagc cgtgctcttt
    47161 aaagatttct tcgagctgca aatcataaag atccgcttga atgaacaccg cattttttaa
    47221 atggaccgtg acgggcttta aatcaacgcc aaggactctg ccttgggccc ccgttttttg
    47281 actggcatat tgcgaccagg atccagggga cgctcccaaa tccaaaatca cttgatgggg
    47341 tttaaaaagt ttgtactttt tgtcgatttc ttcaagctta aaaacggagc gagccgcgta
    47401 gttctcttcc ttagcttttt taaaataatg atcttggggg ttataactca taaaaagcac
    47461 cataacagag gatgcctcgt gaaaaaagca tcctttaagg ttccttaagg aaattgagat
    47521 ctttcagtgt aagaattgca aaaaagagaa gcagagaact aaactctttt aaactggacc
    47581 ccttctctgt tgatggccga gggattgctc tttagctcta gctatgcagc cagagtgtat
    47641 ttattgtagt aaaaagagtc agatatcgga ccaagctccc gaagtcgttt gcttcggaca
    47701 ctttcttcgc gctcgcgacg gaaagaaact tcagagattt cgttgtttgc tttgcagaaa
    47761 aa
//
LOCUS       DATBTE010000371         2605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2442556, whole genome shotgun sequence.
ACCESSION   DATBTE010000371 DATBTE010000000
VERSION     DATBTE010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2442556"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1188
                     /locus_tag="VIG33_14055"
     CDS             223..1188
                     /locus_tag="VIG33_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272010.1"
                     /translation="MGQKFSAIDIGSNAIRMIVGELEHSKDSLQNSSNNNAKITSEMR
                     VIKKLRAPIRLGKDVFMNGVISDKVLSEAQEAFREFAHTNRELKVARCRAVATSAVRE
                     AKNKDLFVKKILTASKVKVEIIDGVEEARLVHTAIRNAIDIENKCILSIDVGGGSVEI
                     TFSENGMMSATESFPMGTVRILDQMNRRKLTENQINVIMGEFVGPLTNYLDSHQIGAG
                     PLHFAIGTGGNLECMAQLKLKILKKLPTTYITIKELSEIAEKLKSMSYKDRMEKLGLR
                     PDRADVILPATLLVLTVLRQARVEKIIIPNVGLRDGLLWSTAGLS"
     gene            complement(1215..2228)
                     /locus_tag="VIG33_14060"
     CDS             complement(1215..2228)
                     /locus_tag="VIG33_14060"
                     /inference="COORDINATES: protein motif:HMM:NF020968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HEY8272011.1"
                     /translation="MAFFGITVEKIGRIWPHSKADKMELAACEGLGFQFCVGKGLYTV
                     GQEVLYFPTDTILPEEFKEKIGIRAPGRIKTVQLRGEYSQGFVFPLDKAKEIFNVDFN
                     VKSPSEITLFFKATKYEPPQKFSTVGELVPLPDGQGIYDIENVDRYLDVYNELLLKSC
                     YITEKLEGTNISISRKEGNIFVCQRANAIIEKPESPNAYWEAARNSGLIEKLQKITDQ
                     DIVLMGELIGPSIQNNIYKVDKPTIILFDIRIARRWLSPPEFYRICNEQNIAAAPILA
                     KGVLKDILAAKTLDEYANGFSQLFNTLREGVVIRPEVEEYDHKLGRVILKKHSLSYKT
                     QEL"
     gene            complement(2236..>2605)
                     /locus_tag="VIG33_14065"
     CDS             complement(2236..>2605)
                     /locus_tag="VIG33_14065"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEY8272012.1"
                     /translation="KEYAVDLLKIAIGDLKTAKALIASPDPGRLENILYMIQQSVEKA
                     VKVVLISKQIPFPLVHDLGILIALLPTSDYPPGGFDLTALNPYSTIRRYETGEIPLDP
                     DEIQGAYSVAELVIAWASKY"
BASE COUNT          810 a          561 c          510 g          724 t
ORIGIN      
        1 caaaggaaga ctgaaagcca taaccccttg atcgcgggta ggaagatttt taagagtcag
       61 ggccaaatct agttctccag aaaaactaat atcgttgact gcaaaggact tggcggaaaa
      121 acctgggaac aaactgtata aaagtgccac tagaatgcaa aatttgattt ttttcataaa
      181 tctcatgctg acactattta aactctgtcg caagctattc atatgggaca aaaattttca
      241 gcgatcgata tcggctcgaa tgccatccgc atgattgtgg gcgagttaga acactcaaag
      301 gatagcttgc agaatagctc gaataataat gcaaagataa cttcggaaat gagggttatc
      361 aaaaagcttc gagcacccat tcggcttggt aaagatgttt tcatgaacgg tgtcatttcc
      421 gacaaagtcc tttcagaagc ccaggaagcc tttcgagagt tcgctcatac caatcgtgaa
      481 ttaaaagtgg ctcgttgtcg tgccgttgcc acaagcgctg tgcgcgaagc aaaaaataaa
      541 gatctttttg taaaaaaaat attaacagcc tctaaagtaa aagtcgaaat catcgatgga
      601 gtcgaagaag ctcgtttagt tcacacggcc attcgcaatg ccattgacat cgaaaataaa
      661 tgcattctat cgatcgatgt tggaggcggc agcgtggaaa tcaccttttc ggaaaatggc
      721 atgatgagtg cgacagaaag ttttcccatg ggaaccgtgc gtattcttga tcaaatgaac
      781 cggcgcaaac tgacagaaaa tcaaatcaac gtcattatgg gagaatttgt agggcccctt
      841 accaactatt tagattccca ccaaattggc gccggccctt tgcatttcgc gatagggact
      901 ggtggtaacc tagaatgcat ggcccaactc aaattaaaaa tattaaaaaa gctccccacg
      961 acatatatca ccatcaaaga attaagtgaa attgccgaaa agctaaaatc gatgagttat
     1021 aaagatcgta tggaaaagtt aggattgcgt cctgacagag cagatgtcat cctacccgct
     1081 acactgttag ttcttaccgt gctccggcag gcgcgggttg aaaagattat tatccccaat
     1141 gtaggccttc gcgatggatt gctttggtct actgcgggac tgagttagaa aacctctgcc
     1201 ttgttataaa acatttaaag ctcttgggtt ttgtaactca aactgtgttt cttcagaata
     1261 accctcccaa gtttgtggtc gtactcttcc acttcagggc ggatgaccac accttctctc
     1321 agagtgttga atagctgact aaaaccattc gcatattcat caagggtttt agcggctaaa
     1381 atatctttta aaacaccttt agcaagaatg ggagccgccg caatattttg ttcattacag
     1441 attctataaa actcaggcgg gctcaaccaa cgtctcgcaa tacggatatc aaataatata
     1501 attgtaggct tatccacttt ataaatattg ttctggatcg acggaccaat caactcgccc
     1561 atcaaaacaa tatcctgatc tgtgattttt tgaagctttt caatcaaccc ggagttgcga
     1621 gcggcttccc aataagcatt tggtgattct ggtttttcaa taatggcatt tgcacgctga
     1681 caaacaaaaa tatttccctc tttccgggaa atagaaatat tcgtgccttc tagtttttcg
     1741 gtaatataac agctttttaa aagcaattca ttatagacat caagataacg atctacgttc
     1801 tcaatatcat aaatcccttg tccatcaggc aacggcacca gctcgccgac agtggaaaat
     1861 ttttgcggcg gttcatattt ggttgcttta aaaaataaag taatttcaga gggtgatttt
     1921 acattaaaat caacattaaa tatttcttta gccttatcta agggaaatac aaacccctga
     1981 ctgtactccc cgcgaagttg aacggtttta atcctccccg gagcgcgaat tccaatcttc
     2041 tccttgaact cttcaggtaa aatagtatcc gtcggaaaat aaaggacctc ttgtcccaca
     2101 gtatagagac cttttccgac acaaaactga aaccccaagc cttcacaagc ggcgagttcc
     2161 attttatcag cctttgaatg aggccaaatt cttccaattt tttctactgt gattccaaaa
     2221 aatgccattt tcctcctaat actttgaagc ccaagcaatg actaattcgg caaccgagta
     2281 agcaccttga atttcatcag gatctaaagg gatttctccc gtctcataac gacgaattgt
     2341 tgaataggga ttcaatgcag ttagatcaaa tcccccaggg ggatagtcgg acgttggcaa
     2401 tagagcaatg agaattccta agtcatgcac taaaggaaaa ggaatttgct tcgaaatcag
     2461 aaccactttc acagccttct ctacagattg ctgaatcata tagagaatat tttcaagtct
     2521 tcctggatca ggagatgcga taagagcttt ggctgttttc aaatcaccga ttgcaatctt
     2581 caaaagatca accgcatatt ctttt
//
LOCUS       DATBTE010000372         4385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_118889, whole genome shotgun sequence.
ACCESSION   DATBTE010000372 DATBTE010000000
VERSION     DATBTE010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118889"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1577)
                     /locus_tag="VIG33_14070"
     CDS             complement(198..1577)
                     /locus_tag="VIG33_14070"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8272013.1"
                     /translation="MNSNNFPRIRFLGGAGTVTGSKCLLTYLDKNILIDCGLFQGLKE
                     LRLKNWDRFPIEPKSIDAVILTHAHIDHSGFIPRLVKEGFCGKVFCTAATLSLCKLLL
                     PDTGHLQQEEAEYLGHHRLSKHHPPLPLFDKAEAENSLKYFVPKNFNEEFEVFPGLTA
                     CFLYAGHILGAAMALIQAGPRRIAFSGDIGRSQDPIFKSPHILPKIDYLIVESTYGNR
                     QHEVTDPLLELEVLIKEAIEKKSVVMIPTFAVGRAQELLYFLSLLRKQGRIPWIPMYL
                     NSPMATDATKIFCEHQSLHKLSPQECLELSNVVHYIQSVEESRALNEKKGPMLILSAS
                     GMATGGRILHHLKAFASDPKNMIIIAGYQALGTRGRSIQDGAKEVKIHRQFVSINAKV
                     ATLENLSAHADYTEIVSWLSKSEIHPAKVFVTHGEKDSASEMRRHLEETFGWKCETPS
                     QDQEFILNN"
     gene            1755..2885
                     /locus_tag="VIG33_14075"
     CDS             1755..2885
                     /locus_tag="VIG33_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272014.1"
                     /translation="MKKVFVFLPLFFLVLSGKAEVKPFMDQMLNEIFNLKPYIVSPEA
                     FSDPKNAQKIDASLKKMVALSVKINHEDKIHKTGFQISGKVLNQQLKEAQIVFNSGSK
                     DYSLWMFKSTLGVCMSCHTQLPAVSTQFMSQNQSKVLSNPFEEAEFLFIIRNFKESMK
                     LYLSALNGYPKNVVSADDLEKVLYRQLYYYVRVQRDLTELAKVLDEDRKNKSLPPHFI
                     SKVKGFKTAVLKMQDEKYPHFTAAQTAQLRQYVEKSLKEELAGNFSFDLPKRELEYLK
                     VSSVLYEYLNENPETSLKPDILYWLSFCESKFSQQAFYSLPELYLKQCVMEFSKNPVA
                     KKCLKEYQDLVVMAYSGSSGTHIPEDISKEMKTMQELVEKIK"
     gene            2912..4171
                     /locus_tag="VIG33_14080"
     CDS             2912..4171
                     /locus_tag="VIG33_14080"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEY8272015.1"
                     /translation="MVQKVTAKKAISFIESHQSIFVHGGVAVPQALIRSLLQRASELE
                     NMEIMHLHTNGDASYAHPQYRGHFKVCNFFVGPNIRPYIDFDSVDYLPCFLSEIPSLF
                     RSGRKKVDVAFLHVSPPDEHGYCSLGTSVDVARAAFESAKLIIAQVNPRMPRVFGDGV
                     IPFDKIHFAVEVDEALPEVPTKPLTEVEKSIGRNVAGLVENGATLQLGIGSIPDAVLA
                     ALDGHKNLGLHTEMWSDGAIPLLEKGIIDNSRKMIHPGKSISSFVIGTKRLYDFIHNN
                     PSVMQLDSSYVNNPSVICRNKKVTAINSAVEIDLTGQVCADSIGRHIISGVGGQIDFI
                     RGASLSEKGKPIIAITSRTHKGIPRIVKMLKEGAGVVTTRAHVHFVVTEYGVAELFGR
                     TLGERAKALIAIAHPEDREALESHWKQ"
BASE COUNT         1205 a          869 c         1034 g         1277 t
ORIGIN      
        1 ggccacgatg gccataattc ggcgttctca cggatggata aacttcgcaa tatcactcac
       61 tgctgagcga agaccttcag cttttcaaaa aaaacataat ttgcttaaat tttaacccac
      121 gaattattca aacgaacccc attttttaac tcacaaaacc cacatccctt aaaaggtgtg
      181 ggtttattta taatcactta gttgttcaat atgaattctt gatcctggga aggcgtttcg
      241 cacttccatc caaaagtctc ctcaagatgc cgtctcattt cactggcgga gtccttttct
      301 ccgtgggtca caaaaacttt tgcagggtgg atttcagatt tagaaagcca gctcactatt
      361 tccgtataat ccgcatgtgc tgagagattt tccaaggtgg cgactttggc gttgatgctt
      421 acgaattgac gatgaatttt cacttccttc gcgccatctt gaatggagcg gcctcgagtg
      481 ccaagggctt gatatccggc aatgataatc atattttttg gatcggaagc gaaggctttc
      541 aggtgatgga gaatgcgccc cccggtggcc atgccactgg ctgagagaat cagcatcggg
      601 cctttcttct cgttaagggc tcgggattcc tccacggatt gaatgtaatg aaccacattg
      661 gagagttcga ggcactcttg aggggacagc ttatgaagag attgatgttc gcagaaaatc
      721 ttcgtggcgt ctgtggccat gggactgttg aggtacatcg gaatccaggg aatgcgccct
      781 tgtttacgta aaagagaaag aaaatagaga agttcttgag cgcgtcccac cgcgaaggtg
      841 ggaatcataa ccacggattt tttttcaatc gcctccttga taagaacttc caattctaac
      901 aaagggtccg tcacttcgtg ttggcggttt ccataggtgg actcaacgat gaggtaatct
      961 attttgggta aaatatgggg agatttaaaa ataggatcct gcgatcttcc gatatcgcca
     1021 gaaaaggcga ttcttcgagg gccggcttgg atgagagcca tggccgcacc tagaatatgt
     1081 ccggcatata aaaagcaagc ggtgaggcct ggaaagactt caaactcctc gttgaagttt
     1141 ttaggaacaa aatattttag agaattttcg gcctccgctt tgtcaaacaa gggaagcgga
     1201 gggtgatgtt tggaaagcct gtgatgcccc aagtattccg cctcttcctg ttgtaaatgt
     1261 cccgtatccg gcaacagcag tttgcaaagg gagagggtgg cagctgtgca aaagactttg
     1321 ccacaaaagc cctctttgac caggcgggga ataaaacctg aatgatcgat atgagcatga
     1381 gtcagaatca ccgcatcgat actctttggc tcaatgggga agcgatccca gtttttaagg
     1441 cggagttctt tgaggccttg aaaaagcccg caatcgatca aaatattttt atcgaggtag
     1501 gttaaaaggc attttgaacc agtgacggtt ccggctcccc ctaaaaacct gattctagga
     1561 aaattattgc tgttcatttg aaataagcct catcctcagt gttagaatat attttcatat
     1621 taaaagacaa aactgtcatt atgccacaac tatttttgca ggatagattt tcacggtaca
     1681 gatttacggt acagatattt gcaggactct tgcttttatt tctttaggcg ggttaaatgg
     1741 gcgagggggg tattatgaaa aaagtttttg tttttttgcc tttgtttttt ctagtcctgt
     1801 ctggcaaagc cgaagtgaag ccattcatgg atcaaatgtt aaatgagatt ttcaatctta
     1861 agccctacat tgtttcacca gaggctttta gtgatcctaa gaacgcgcag aaaattgatg
     1921 cctctttgaa aaagatggtg gcgttgagtg tgaagatcaa tcatgaagat aaaattcata
     1981 agaccggatt tcaaatttct ggtaaggttc tgaatcagca gcttaaagag gctcaaatag
     2041 tttttaattc aggaagcaag gattactctt tgtggatgtt taaatccacc ttgggtgttt
     2101 gtatgtcttg tcatacacag ttgccggcgg tatcaactca gtttatgagt caaaatcaaa
     2161 gcaaagtttt atcaaaccct ttcgaagaag cggaattttt atttatcatt cgtaacttca
     2221 aagaatcgat gaagttgtat ctgagtgctc tcaatggata tccaaaaaat gtggtctctg
     2281 cggatgacct tgaaaaggtt ctctatcgcc agctttacta ttatgtcaga gttcaacgag
     2341 accttactga gcttgcgaaa gtcttggatg aagatcgaaa gaacaaaagc cttccgcctc
     2401 attttatttc caaagtaaaa ggttttaaaa cggctgtgtt aaagatgcag gatgaaaaat
     2461 atcctcactt cacggccgct cagacggcgc agttaaggca atacgtggaa aaatccttga
     2521 aagaagaact ggcgggaaat tttagcttcg atttgccaaa aagagagctg gaatacttga
     2581 aggtgtcgag tgtcctttat gagtatctca atgaaaatcc agaaacctcg cttaagcctg
     2641 atattcttta ttggctttcg ttttgcgaat ctaaatttag ccaacaagct ttctattctt
     2701 tgccagagct ttatttaaaa caatgtgtca tggagttttc taaaaatccc gtggcgaaaa
     2761 aatgtttgaa agaatatcaa gatctggtag tgatggccta tagtggatcc agcggaacac
     2821 atattccgga ggacatttcc aaagagatga aaaccatgca ggaactcgtc gagaaaatta
     2881 aataattgga agtaggattg aagaggcgga gatggttcaa aaggtcactg cgaaaaaggc
     2941 tatttctttc attgagtccc atcagtccat ttttgttcat ggcggcgttg cggttcctca
     3001 ggctttaata aggagtctgt tgcaacgggc ctctgaattg gaaaatatgg agatcatgca
     3061 tctccataca aatggtgatg cctcctatgc tcatccccag tatcgggggc attttaaagt
     3121 atgtaacttt tttgtgggcc cgaatattcg accttacatt gattttgatt ctgtcgatta
     3181 tctgccttgt tttttatctg agattcccag tttatttcgc agcggtcgaa aaaaagttga
     3241 cgtggccttt ttacatgttt cgcctcccga tgagcatggt tattgttcct tagggacgag
     3301 tgtggatgtg gcccgcgccg cttttgaatc agccaagctt attattgccc aagtaaatcc
     3361 tcgcatgccg agagtatttg gggatggagt tattcccttt gataaaattc atttcgccgt
     3421 tgaggtggat gaagctttgc cggaggttcc tactaaaccc ctcactgaag ttgagaagtc
     3481 cattggtcga aatgtggccg gtcttgttga aaatggtgcc actttgcagt tgggtattgg
     3541 atctattccc gacgcggttt tagcggctct cgatgggcat aaaaatttag gactgcatac
     3601 ggaaatgtgg tctgatgggg cgattcctct tttggaaaag ggaatcattg ataattctcg
     3661 gaagatgatt cacccgggaa aatccatttc tagctttgtc atcggaacaa aaagacttta
     3721 cgattttatt cataacaatc cttctgttat gcagttggac agcagttatg tgaataatcc
     3781 cagtgttata tgtaggaata aaaaagtcac agcgattaac tctgcggtag agatcgattt
     3841 aacagggcaa gtctgtgcgg attccatcgg gcgtcatatt atttctggag ttgggggaca
     3901 gatagatttt attcgcggag cttccttgtc tgagaagggc aagccgatta tcgccatcac
     3961 ctcacgcacc cataaaggaa ttccaagaat cgttaaaatg ttgaaggaag gcgctggtgt
     4021 tgtaaccacc agggcccatg ttcactttgt ggtgactgag tatggagttg cggaattatt
     4081 cggccggacc ctcggagagc gggcaaaagc ccttattgcg atcgcccatc cggaagatcg
     4141 ggaagcttta gaaagccact ggaaacagta gatctccgaa gcttaatgtt tgaaaactgg
     4201 cccccttctc tgttgatggc cgagggattg ctctttagct ctgtctatgc agccagagtg
     4261 tatttattgt agtaaaaaga gtcagatatc ggaccaagct cccgaagtcg tttgcttcgg
     4321 acactttctt cgcgctcgcg acggaaagaa acttcagaga tttcgttgtt tgctttgcag
     4381 aaaaa
//
LOCUS       DATBTE010000373         5970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_118983, whole genome shotgun sequence.
ACCESSION   DATBTE010000373 DATBTE010000000
VERSION     DATBTE010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118983"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /gene="grpE"
                     /locus_tag="VIG33_14085"
     CDS             <1..141
                     /gene="grpE"
                     /locus_tag="VIG33_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEY8272016.1"
                     /translation="NYMEALSSEPTNEIPPGHVARVFKKAYKLHDKVIRPAQVVVAKQ
                     LE"
     gene            156..1145
                     /locus_tag="VIG33_14090"
     CDS             156..1145
                     /locus_tag="VIG33_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEY8272017.1"
                     /translation="MSKKDFYSLLNVPRSATADEIKKAYRKLAMQYHPDKNPGDKKAE
                     EKFKEFSEAYEVLSDPKKREMYDQFGHAGAQNGFGGASGPFGGAGGFGSSAGTGQDPF
                     QDIFGDVFSEIFGARGHGPGASAGGPRRRQSKGSDLRYTLNISFEESALGAEKVISFV
                     RQRGGKDETAKLSVNVPAGVKEDQRLKLAGEGDTSLSGGPPGDLYVIIKVQEHPLFKR
                     QDNDIVIDLPVTYTDAILGTSVEIPTLTGKAMIRIPPGTHTGQTFRLKGKGFPKIGGF
                     GSGDMLVRLIVDTPEKISAHQKELLEELSKVGEETPLVKAFKEKVVNIMRNKK"
     gene            1142..2836
                     /locus_tag="VIG33_14095"
     CDS             1142..2836
                     /locus_tag="VIG33_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEY8272018.1"
                     /translation="MRTGFLLLSTLTLVSCMGVQTKRPEVPQKNGRHAGVTPLQSSSQ
                     TTLGKQMGGMGQQLPPQAKTNSIKVHELEIAGDYMEALKQSVNFSVTANTPQEQESFR
                     LKSIEIVEGKLNQEQLGKVAHDSDFGFTRGYALFRLGETALEERDKDHAKKYFSSVGE
                     FLPGSDLALRAQDILSQLESSKLVESKTVGVVLPLTGKNSAMGQKALRGVEMGLGLNN
                     PGSGFRLAVMDSEGNPDNARRGVERLIKEDNAIAIIGSLLSKTAPAVAAKADELGVPT
                     IGLSQKSGLTELGPTVFRNALTSEMQVHYLVRTAMEDYGLKRFAIVYPNDPYGIEYTN
                     LFWDEVLARGGTVTAVQTYSPKETDFRFPVQRLVGTYYIEARLDEYKVRTKQNAESDK
                     KKSVRISHEEEVLPPIVDFDAVFIPDSAKSMGQIAAFLSFAGVKGVKLLGTNLWNTPG
                     LAKRAGNFANNLIFVDSFLPSTATHSRFVQEYKTTFNEEPSLIEIQAYDSALILRQLI
                     AQGAATRESLTRELTSLKKFPGALGTLNMSPDREIQRPLMAITLEKGEIVPLKIQK"
     gene            2926..3300
                     /locus_tag="VIG33_14100"
     CDS             2926..3300
                     /locus_tag="VIG33_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEY8272019.1"
                     /translation="MNTTLNQQTIFECRKKLILLRQDLLNRVRMSQLDFGHVEKSSGD
                     EVDQTVAQLAENHFLISQERMYIQLLEIDQALARIENGTYGICEETEEPIEIERLFAI
                     PWTRLSIEGAEIQEAMLRKFAR"
     gene            3478..3837
                     /locus_tag="VIG33_14105"
     CDS             3478..3837
                     /locus_tag="VIG33_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015471284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEY8272020.1"
                     /translation="MNSTELSEIRESLLSQKSAILNKTSEFKKEQSRAVMVSDEAEAA
                     SIDLSNNISIHLHERDRNSLIMIERALGKISEGTYGQCECCGGLISARRLQARPFTTM
                     CVECMEDQEDLRNSYQQ"
     gene            4003..>5970
                     /gene="lon"
                     /locus_tag="VIG33_14110"
     CDS             4003..>5970
                     /gene="lon"
                     /locus_tag="VIG33_14110"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEY8272021.1"
                     /translation="MSFDEKVLEIPQTLPMLPVRDIVVFPYMIIPLFVGREASIRSVE
                     EALAKNRLIFLASQKDINEENPSPDTIYTVGTVAMIMRMRKLSDGRVKILIQGVAKAR
                     VKNYVKTTPSFEVAVDKIHETPSQLPAVESEALIRTAKEHIERIIALGRPLSPDILLV
                     LDDVNDPGRVADLVASNLGVKVQDAQKVLETSDPKERLKIVNELLAAEIEVMQMQTKI
                     RTNAKDEMSKTQREYFLREQMKAIKSELGEQDSKSEEMEDLREKLVNAGMPPQVEAEA
                     LKQLARLERMHPDASEATMVRTYLDWMVDLPWAKKTEDVLDLKKAKEILDDDHYDLQK
                     AKDRVLEFLAVRKLKTSMKGPILCFAGPPGVGKTSLGRSIAKSMGREYFRLSLGGVKD
                     EAEIRGHRRTYVGAMPGKVIQALRQAKTNNPVIVLDEVDKLGSDFRGDPSAAMLEVLD
                     PEQNSTFRDNYLNVDFDLSNVLFIATANMLENIPPALRDRMEIINIPGYTENDKLLIA
                     KKHLIQRQIEANGLTSENVVFDDEGLRYLISGYTREAGLRNLEREIGSICRKVAKMVV
                     MEETKFIQVNALTVPELLGPPRFLREDKLADSQVGIIQGLAWTQAGGEVLYVEALKMK
                     GKGNLLLTGQLGDIMKESAHAAMSYAKAHMDE"
BASE COUNT         1751 a         1264 c         1354 g         1601 t
ORIGIN      
        1 aactatatgg aagcattgag ttcagagccc actaatgaaa ttcctccggg acatgtggcc
       61 cgggttttca aaaaagctta taaacttcat gataaagtga ttcgacctgc acaagttgta
      121 gtcgcgaaac aattggaata aataggagct gatttttgtc taaaaaagat ttttattctc
      181 ttttaaatgt gcctcgctct gcgactgcgg atgaaattaa aaaagcctac cgcaagcttg
      241 caatgcagta ccatcctgac aaaaatccag gcgacaaaaa agcagaagag aaatttaaag
      301 aattttctga agcttatgaa gtcctgagtg atccaaaaaa acgcgaaatg tatgaccaat
      361 ttggacatgc tggagcccaa aatggctttg gcggagctag cggtccattt ggcggagccg
      421 gcggatttgg aagcagtgcc ggtactggtc aagatccttt tcaggatatt ttcggcgatg
      481 tatttagtga aatatttgga gctcgcggac atggcccagg cgcaagcgct ggaggcccac
      541 gtcgacgcca atctaaggga tctgatcttc gctacaccct taatatttct tttgaagaat
      601 ctgctctggg ggcggaaaaa gttattagct ttgtccgtca acgtggcggt aaagatgaaa
      661 cggcaaaact ctctgtaaat gttcctgctg gcgtaaagga agaccaacgc cttaaacttg
      721 ctggagaggg cgatacttca cttagtgggg gacctccggg agacttatac gttatcatta
      781 aggtgcaaga acatcccctt tttaaaagac aagacaacga tatcgtcatc gatcttcctg
      841 tcacttatac agatgcgatt ttaggcacct ctgtggagat tccaaccctc accggaaaag
      901 ccatgattcg tattccaccc ggcactcata caggacaaac tttccgcctt aagggtaaag
      961 gtttccctaa aatcggtggc tttggctcag gggatatgct ggtccgatta atcgtcgaca
     1021 cgccagagaa aattagtgct catcaaaaag agcttctcga ggaactctcc aaggttggag
     1081 aggaaactcc gctcgtgaaa gcgtttaaag aaaaagtcgt caatattatg aggaataaaa
     1141 aatgagaact ggatttctgc ttctctctac attgaccctg gtctcttgta tgggtgtgca
     1201 aacgaaaagg cctgaggtac cgcaaaagaa tgggcgccac gctggagtaa cgccccttca
     1261 gtcttcttcg caaaccaccc ttggtaaaca gatggggggg atgggccaac aacttccgcc
     1321 ccaagcgaaa accaatagca taaaggtgca tgagctggaa attgcaggcg attatatgga
     1381 agccttaaaa cagagtgtaa atttctctgt gaccgcgaac actccccaag agcaagaatc
     1441 cttccgctta aaaagtattg agattgtcga gggcaaactc aaccaagaac aacttggaaa
     1501 agtagctcac gattctgatt tcggtttcac tcgtggatat gctcttttcc gtctgggtga
     1561 aacagcttta gaagagcgcg acaaagatca tgcaaaaaaa tatttttcaa gtgtgggcga
     1621 atttttgcct ggcagtgact tagctcttcg tgctcaagac attttatctc aactggaaag
     1681 cagcaagctt gttgaatcaa aaactgttgg agttgtactt ccgctcactg gaaaaaactc
     1741 agctatgggc cagaaggcgc ttcgtggagt ggaaatgggc ttaggactca ataaccctgg
     1801 ttctggtttc cgtctggctg ttatggatag tgaaggaaat cccgacaatg ctcgtcgcgg
     1861 agtagagcgc ttgattaaag aagataatgc tattgcaatt attggaagtc ttttaagcaa
     1921 aaccgcgcca gcagtagctg ctaaggccga cgaactgggg gttccaacca ttggtctttc
     1981 acaaaagtca ggactcactg agttgggccc cactgttttt agaaacgcgc ttacaagtga
     2041 aatgcaagtt cactacttag ttcgaactgc tatggaggac tatggcttaa aacgttttgc
     2101 cattgtttat ccgaatgatc cctacggcat agaatataca aatttatttt gggacgaggt
     2161 tctggctcgt ggcggtactg tgactgcggt gcaaacctac agcccaaaag aaacagattt
     2221 ccgtttcccc gttcaacgcc ttgtggggac ttattatatc gaagcccgtc tggatgaata
     2281 caaggtccgc actaaacaaa atgctgagtc cgacaagaag aaatccgtcc gcatttctca
     2341 tgaagaagaa gtcctccctc ccattgttga tttcgatgct gtgtttattc cggatagtgc
     2401 aaagtctatg ggccaaatcg ccgccttttt atcttttgca ggtgtcaaag gtgtgaaact
     2461 tttagggacc aatctttgga atactcccgg tcttgctaag cgtgctggta attttgcaaa
     2521 taaccttatt tttgtagaca gcttcctacc ctccacagca actcattcac gctttgtaca
     2581 ggaatataaa acaactttta acgaagagcc ttctttgatt gaaattcaag cgtatgattc
     2641 tgctttaata ctgcgtcagc tcattgctca aggagctgcg actcgagagt ctttaactcg
     2701 tgaattgact tcattgaaaa aatttcccgg agcattaggt acgctgaata tgagtccaga
     2761 tcgtgagatc caacgtcctc tcatggcaat tactcttgaa aaaggcgaga ttgtgccttt
     2821 aaaaattcaa aaataacatc tttgctaacc ggccccctaa gaacgactgt cttagtcctc
     2881 aatagtatgt aaatacacat actgaataca taaaggaggg ggccagtgaa tacgacttta
     2941 aaccaacaaa caatcttcga gtgcagaaaa aaattaatct tattgagaca agaccttctc
     3001 aatcgtgttc gtatgtctca actagacttt ggccatgttg aaaaaagcag tggtgatgag
     3061 gttgaccaaa cagtcgctca acttgcggaa aatcatttcc ttatttcaca agagagaatg
     3121 tacattcagc ttctcgaaat agatcaagct cttgccagga ttgagaatgg cacatacgga
     3181 atttgtgaag aaacggaaga acccatcgag atcgaacgcc tctttgctat tccatggact
     3241 cgtctcagca ttgagggagc tgaaattcaa gaagctatgc ttcgcaaatt tgcacgctaa
     3301 aaattttttg ttcccctcgc gaggcgttag tttgtgctga gaattttagc actctgattt
     3361 tttttgatga cactccttaa agcctttgta agatgcgctt tcagttttcg gccaaggatc
     3421 atccttaagt taattgtgtg aagcgattca ttcagcaagc ttacgaaggg ggtaaagatg
     3481 aactcaaccg agctaagtga gatcagagaa tctcttcttt cacagaagag cgctattctg
     3541 aacaaaacaa gcgagtttaa aaaagaacaa tcgcgtgccg tcatggtttc tgatgaagca
     3601 gaagctgcgt ctattgattt atctaacaac atttccattc accttcatga gagagaccgc
     3661 aattctctga tcatgattga gcgtgccctc ggaaaaatat ccgaaggtac ttatggtcaa
     3721 tgcgaatgtt gtggaggttt aatcagtgcc cgccgcctcc aagctagacc ttttaccact
     3781 atgtgcgttg aatgcatgga ggaccaagag gatttacgca actcctatca gcagtagctt
     3841 catgtttaac tcattggatg aggccttctg acatggatgt cagtcttgat tttattgatt
     3901 ttctttagac ctggccattt ttttattaaa gcctttttgt tgcacctccg acaagtgttt
     3961 caagcaagaa acacttaatt tccgcttcag cggaggagcc acatgagctt tgacgaaaaa
     4021 gtactagaaa tcccgcagac actacccatg ttgccagttc gagatatcgt tgtatttcct
     4081 tatatgatca tacctttgtt tgttggtcgt gaggcttcga tccgttctgt agaggaggct
     4141 ttagcaaaaa atcgccttat cttcttggct tctcaaaaag atataaacga agaaaatccg
     4201 tctccggata ctatctatac agtaggtacg gtagcgatga tcatgcgaat gagaaaactc
     4261 tctgatggtc gcgtaaagat cctcattcaa ggcgttgcta aagctcgcgt gaagaattac
     4321 gttaaaacta ccccaagctt tgaagtggcg gttgataaaa ttcatgaaac cccgtcacaa
     4381 cttccagcag ttgaatcaga agccctgatt cgaacagcga aagagcatat cgaaagaatc
     4441 attgctctgg gtcgccctct atctccggat attttgttgg ttctagacga cgtcaacgat
     4501 ccaggtcgtg tcgccgatct tgtggccagt aatctagggg tgaaagttca agatgcacaa
     4561 aaggttctag aaacttcaga ccctaaagaa agacttaaaa tcgtcaatga gttattggct
     4621 gcggaaatcg aagttatgca aatgcaaact aagattcgta ccaatgcaaa agatgaaatg
     4681 tctaagactc aacgtgaata tttcttgcgc gaacaaatga aagctataaa aagcgaactt
     4741 ggcgagcagg actcaaaatc cgaggagatg gaagatctcc gcgaaaaact cgtgaatgca
     4801 ggaatgcctc cgcaagttga agctgaagct ttgaaacagc tggctcgtct tgaacggatg
     4861 catccagatg ccagtgaagc cacaatggtt cggacttatc tggactggat ggtagacctt
     4921 ccttgggcta agaaaacaga agacgtttta gatcttaaaa aagccaaaga aattttggat
     4981 gatgatcatt atgatctgca gaaagctaaa gaccgtgttc ttgaattctt agcggtacgc
     5041 aaactgaaaa caagcatgaa aggtcctatc ctttgttttg ctggtcctcc aggtgtaggt
     5101 aaaacttccc taggccgttc tatcgctaaa tcaatgggcc gcgagtattt ccgactttca
     5161 cttggcggag taaaagacga agcggaaatt cgggggcacc gtcgaacata tgtaggtgct
     5221 atgcctggta aggtcattca agcacttcgc caagctaaaa ctaacaatcc tgttattgtt
     5281 ctggatgaag tcgataagtt gggttctgat ttccgcggag atccttctgc agcgatgctt
     5341 gaggtattgg atcctgaaca gaattcaacc ttccgagata attacttaaa tgtagatttt
     5401 gatctttcaa atgtcctttt tatcgccaca gccaacatgt tggaaaacat tccaccggct
     5461 ttgcgtgacc gtatggaaat cattaatatt cctggttaca ctgaaaatga taagctttta
     5521 atcgctaaaa aacatttaat tcaaagacaa attgaagcca atggtttgac cagtgaaaat
     5581 gtggtcttcg acgatgaggg cctccgatat cttattagtg gctatactcg cgaagccggt
     5641 cttcgtaacc tagagcgcga aataggctct atctgccgca aagtagctaa aatggttgtt
     5701 atggaagaaa cgaaattcat ccaagtcaac gcactcacag taccggagct cttggggcct
     5761 ccacgcttct tgcgtgaaga caagttggct gattcccaag ttgggatcat tcaagggctt
     5821 gcgtggacac aagccggtgg cgaagtactt tatgttgaag cccttaaaat gaaaggcaag
     5881 ggtaacttgt tgctgactgg ccaattaggc gacattatga aagagtccgc ccatgcagcc
     5941 atgagttatg caaaagcaca tatggatgag
//
LOCUS       DATBTE010000374         4279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2374297, whole genome shotgun sequence.
ACCESSION   DATBTE010000374 DATBTE010000000
VERSION     DATBTE010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2374297"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1503
                     /locus_tag="VIG33_14115"
     CDS             <1..1503
                     /locus_tag="VIG33_14115"
                     /inference="COORDINATES: protein motif:HMM:NF016158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HEY8272022.1"
                     /translation="MKTSKGLFLIQSEQLFSTAYLKGHENDLFLMIEDRQQLTTYKFH
                     KHKLIFYLSAMRHYAREIRRQDYNLVYLELAETDGLPYEKILERVLKESSLDKIITFE
                     IEDKDHENKILEFCARNAYTLEIKTSPQFLVSRTHFKNYLEQHPQPFLKSFYEDQRRK
                     LKILVEPNGEPIGGRFSFNESSHLKCPHEHGTPKFPFPGHDEIDHEVIKLVDKEFSDH
                     PGESLTAWYPTSRKTAHKTLRDFCKYRLAEFAPYEETLCPGEDFLFHSVLSPLLNVGL
                     LTPREVLTAAVQSTSEIPIPLNSLESFVKKIIGYREYARGIHQNFSETQEQSNFWKHF
                     RLPNENWRLGKTEIPPLDDAIKKVLRLSYIHHTERLKIICNMMNLAEIAPLEAYLWFM
                     EMQLDSVAWATGPNVYGMGLHSDGGIFTNDLHICKANYWLKISTYTKDEWCQEVDGLY
                     WRFIDKHHAFFLKNPKLEAMAKTLEHITPERKEQLWRAADAFIQRNTSYP"
     gene            complement(1548..2381)
                     /locus_tag="VIG33_14120"
     CDS             complement(1548..2381)
                     /locus_tag="VIG33_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY8272023.1"
                     /translation="MGMRITTNVAALNAQKNLGMTQRSMNTSMARLSSGMRINQAADD
                     AAGLAISENLKAQIRGLRQANRNANDGISLVQVAEGSLNEVSNMLIRLRELGVQAASD
                     TIGDTERKFLDVEYQQLKSEIQRVTESTNFNGYDLLNGTGGIIDIQVGTHNDPFKDRI
                     SFNASAANATLESLGLVSESAATKEGAQLSLDVVDQALVSVNAIRANFGAMQNRLTST
                     SNNLLVSDENLSAANSRIRDTDVAAETSEMTRNSILLQAGISVLGQANNSQQMALKLL
                     G"
     gene            complement(2837..3685)
                     /locus_tag="VIG33_14125"
     CDS             complement(2837..3685)
                     /locus_tag="VIG33_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY8272024.1"
                     /translation="MGLRINTNTASLNSQRVLWGTKLGLDKSMERLSSGFRINRAGDD
                     AAGLAISENLRAQIRGLKQSSRNAQDGISLVQVAEGSMNEISSILIRLRELSVQAASD
                     TVGPTERQFLNVEYDQLTSEIDRIAEGTEFNGTQLLAGVGSILDFQVGTRNNPEIDRI
                     SFDASKADASSAALGVNLTSVADKASAQNALAAIDTAIVSVSAMRADFGAIQNRLTST
                     VSNIQVSVENMSAANSRIKDVDVAEETSEMTRNNILLQAGTSVLAQANQQANVALSLL
                     NKSFQG"
BASE COUNT         1213 a         1018 c          846 g         1202 t
ORIGIN      
        1 atgaaaacct ctaagggtct ttttctcatc caaagtgaac agttattttc aactgcctat
       61 ctcaagggac atgaaaatga tctttttcta atgattgagg atcgtcagca actgacgact
      121 tataaatttc ataagcacaa attaatattt tatctctcgg caatgcgaca ttatgctcgc
      181 gaaattcgtc gtcaggatta caatttagtc tatctcgaac tggcagaaac cgatggcctt
      241 ccttatgaaa aaatcctcga aagagtcctc aaagaaagtt ctttggataa aatcattact
      301 ttcgagattg aagacaaaga ccacgaaaat aaaattctgg agttttgtgc tcgaaatgca
      361 tatacactcg aaattaaaac cagtccgcag tttctggttt ctcgcactca ttttaaaaat
      421 tacctagagc agcatcctca accatttcta aagagctttt atgaagacca acgtcgaaaa
      481 ttgaaaatct tggtagaacc caatggggaa cccataggtg ggcgtttcag ttttaacgaa
      541 agcagtcatc ttaaatgtcc tcatgagcat ggaactccaa agtttccttt tccaggtcat
      601 gatgagattg accacgaggt tataaaactg gtcgataaag agttttctga ccatccaggt
      661 gagtccctta ccgcctggta tcctaccagc cgcaaaacgg ctcataaaac actcagagat
      721 ttttgcaagt atcggctagc ggaattcgcg ccctatgaag aaaccctttg ccctggtgaa
      781 gattttctct tccactcagt tctttcacca ttgttaaatg tggggcttct cacccctcgt
      841 gaagtcctca ctgcggcggt gcagtccact tcagaaattc caattcctct taattcactg
      901 gaaagttttg taaaaaaaat cattggctat cgcgagtatg cccggggcat tcatcaaaac
      961 ttcagtgaga cccaagagca aagcaacttc tggaaacact tccggcttcc caacgaaaac
     1021 tggcgtctcg gaaaaaccga aatccccccc ctggacgacg cgataaaaaa agtacttcgg
     1081 ctttcctata ttcatcatac agagaggctt aaaattatct gtaatatgat gaaccttgca
     1141 gagattgctc ccctcgaagc ttacctctgg ttcatggaaa tgcaactaga ctccgtggcc
     1201 tgggcgaccg gccccaatgt ttacggcatg ggacttcatt ctgacggagg aatttttacc
     1261 aatgaccttc atatctgcaa ggcaaattac tggcttaaaa taagtaccta cacaaaagac
     1321 gaatggtgcc aagaggtgga cggtttatac tggcgcttta ttgataagca ccacgccttc
     1381 tttttaaaaa acccaaagct cgaagcgatg gccaagactc tcgaacatat cacccccgag
     1441 cgcaaggagc agctctggag agcggcggat gctttcattc aaagaaatac atcctaccct
     1501 tgattaatac cttcaagcct tagccatctt catcttttat cctaacttta tcctaacaac
     1561 ttcaaagcca tttgctgaga gttatttgct tggcctaaaa ccgagatccc cgcttgtaac
     1621 aagatactat ttcttgtcat ttcagaagtc tccgcagcaa catctgtatc acgaatacga
     1681 gagttcgcag ctgataaatt ttcatccgaa accaacaagt tattggaggt tgaagtcaga
     1741 cgattttgca tggctccaaa gtttgctcga atcgcattca cagagaccaa ggcctgatcc
     1801 accacatcta aactgagctg ggctccttct ttcgtcgcag cactttcaga caccagtcct
     1861 aaggactcta gagtcgcatt tgctgccgaa gcattgaaag agattcgatc tttaaagggg
     1921 tcattgtggg taccgacttg gatatcgata attccacctg ttccattcaa aagatcatag
     1981 ccgttgaaat tcgtggattc cgtcacccgt tgaatctcag attttaattg ttgatactcc
     2041 acgtccaaaa actttctctc ggtatcacca atcgtatcag acgccgcctg aactcctaat
     2101 tctcttaggc gaatcagcat atttgaaact tcgttcaaac tgccctccgc gacttgcacc
     2161 agtgaaatcc catcattggc atttctattg gcttgtcgca ggccccgaat ttgcgctttc
     2221 aggttctctg aaatagcgag acccgcagca tcgtcggcgg cttgatttat cctcattcct
     2281 gaggacaatc gtgccattga cgtgttcatc gatctttgcg tcatccccag atttttttgg
     2341 gcattcagcg cagcgacgtt ggttgtaatt ctcataccca ttttaccctc cttgtaacat
     2401 cagatcctcc aatgatcttt gccatccctt ggcaataagg cgcttcatgc acctgcaatt
     2461 gtttattaaa tttagtatag aacccggttt cttatcggaa taattccaaa aaatttcgag
     2521 tccagttttt ttcaattcaa ataaaatgca aaaatgaaat cttttacgaa gatttcttct
     2581 gacatcaaag cacattctat gcttcgaaga gatgaaggat ccttgcccca agatgcatcc
     2641 atgcatctga aagaagttaa aaagcaagat ccattttaag tccaaaacct caaatatggg
     2701 gttgcaaggc aaccccatgc gcaggaaaca gatatcaata cagtcaacac gtcatgtgta
     2761 acaagcctta gctcttgagg gtgatcttga agttcaagac cgggaacctt atcattcaaa
     2821 gatcaatccg taaggattaa ccttggaagg atttgttcaa gagtgaaagt gctacatttg
     2881 cttgctgatt cgcttgtgcc aagacagaag taccggcttg taacaagata ttatttctag
     2941 tcatttcaga agtctcttcc gctacatcca catctttgat acgactgtta gctgcggaca
     3001 tgttctcgac actcacctga atattactta cagtggaggt cagacgattt tgaatcgcac
     3061 cgaagtcagc tcgcatcgcc gagactgata cgatcgcagt atcaatcgcc gccaaggcgt
     3121 tttgtgctga agctttatcg gccacgcttg ttaagttaac ccctaaggcc gcggaactgg
     3181 catcggcttt agaagcatca aaagaaatcc tgtcaatttc gggattattt ctggtcccta
     3241 cttggaaatc caagatagag ccgacccctg ccagaagttg ggttccattg aactccgttc
     3301 cttcggcgat acgatcgatc tctgaagtca gctgatcgta ttccacgttc aggaattggc
     3361 gctctgtggg cccgaccgtg tcggaagccg cttgaacaga aagctctcgg aggcggatca
     3421 agatcgaaga aatttcattc atcgatccct ctgccacttg caccagagag ataccatctt
     3481 gggcatttct agaggattgt tttaaaccgc gaatttgggc tctgagattt tcagagatcg
     3541 cgagtccagc ggcatcatca ccagctcggt tgatgcggaa acccgacgag agcctttcca
     3601 tgctcttatc gagacctaac ttcgttcccc acagaaccct ctgcgaattt aacgaagctg
     3661 tattcgtatt aatacgaaga cccataattc ctcctccttg aagattcaca agtttcgatc
     3721 cttgaaactt gggatgccgt cttagcacct gaatgggtag ttactcttac ctcactcatc
     3781 ggcgtctctt gaaaatccta aagtgagatt atctcttttt aagaaaaact ggacttaagg
     3841 ctttaatttt aatgaggagg tgaaggatga ccactacaaa gacaaagcct gcgcatttct
     3901 gagtaggctt tgtcttgaaa acaagtgggc ctgttgaaaa accatcagct ttaaatcggc
     3961 aatctaaaat caagatcgaa ctaaaattgt gacgtagaat cagaccattc atcgctagaa
     4021 catcggccgg tcaatccttt tcaaggggga ctaggtgaag gcgccattca gacaaaaact
     4081 tctgtataca agtaaggatc ctgcggcgat gcaggttttc aaatgaactt ctcccctttt
     4141 tggttttaaa tttcctcgta catttatcct cgtacatttg taaaattctc tccgttttat
     4201 agaagggtct taaattttct actagacgaa gatacggcta tcataagtcg cgaaggcgct
     4261 tattattcat tttgaaact
//
LOCUS       DATBTE010000375         2698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1212167, whole genome shotgun sequence.
ACCESSION   DATBTE010000375 DATBTE010000000
VERSION     DATBTE010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212167"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..776)
                     /locus_tag="VIG33_14130"
     CDS             complement(105..776)
                     /locus_tag="VIG33_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272025.1"
                     /translation="MRKQRECKLRLRLQAKPALQAITRSFYAGSQAPAWEPSEQSSSF
                     ASREARASRTEFPSRSLGTSTNFLRSCATITSFPRAAWECRIGRAASYICKADISTNL
                     TTTRGAARLDCIPTQRVGTRHDNKRCAKTKKICLYYDFNPVCRAEYRSFRREQPAGAR
                     QEPRVDRGPGMALLATPGESEERRKQAATGSPFFWILFFEGVDKINKSLNFNDKNIGT
                     YLFIF"
     gene            complement(1293..1718)
                     /locus_tag="VIG33_14135"
     CDS             complement(1293..1718)
                     /locus_tag="VIG33_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272026.1"
                     /translation="MDVTTVLSSIKTATEIAKLIKNSVDTLEKAESKLKLADLISALA
                     EAKIQVTEIQQILLEKDAKLKVLQEQLTKKEMLQWESPYYWLIDGTLKDGPFCQHCYD
                     KNLDIIRLQGNGKGFWECKACKNSYKDKSYNHNASSSMW"
     gene            complement(1786..2487)
                     /locus_tag="VIG33_14140"
     CDS             complement(1786..2487)
                     /locus_tag="VIG33_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272027.1"
                     /translation="MTIAEFLCFLNTNEGVFSTIFSGIVMLATVVYAFLTHSLVKETK
                     HMRELQTKPKIEVIAFLKEEFVNIITLRVKNIGLGPALDVCFRLEGESQTDGEKELIQ
                     DFSRSQFLMTGLRYLGPGQELQTRYTEITENYSEKIKARLMIEVTYNSSAGKSYKDKI
                     PVYFEEFEGYGTLGTPHLYAIAQSLEKIEKNLDHLATGFKRLKTDTYSEKDRRRERQD
                     LEEKMERYQQNNTNS"
BASE COUNT          678 a          648 c          598 g          774 t
ORIGIN      
        1 ttgattgaat cggggtctcg cccccgaggg cgagatactt ttctttgctc cgccaaagag
       61 ggtgtagaca aaatattttt aactaattga ttttaaagtg cactttagaa aatgaatagg
      121 taggtaccta tatttttatc attaaaattc aatgacttat ttattttgtc tacaccctca
      181 aagaaaagta tccaaaagaa aggcgacccg gttgccgctt gcttcctgcg ttcctcgctt
      241 tcgccggggg ttgccagaag ggccatccct ggccctctgt caacgcgcgg ctcctgccgc
      301 gcccctgcgg gctgttcccg gcgaaagctc cggtactcgg cgcggcagac gggattgaag
      361 tcgtagtaca agcaaatctt cttcgttttc gcacagcgtt tattgtcatg tctcgttccc
      421 acgcgctgcg tgggaatgca gtcaaggcgc gctgcgccgc gagttgtggt taaattcgtt
      481 gaaatatcgg ccttacaaat ataggacgca gcgcgtccta tacggcattc ccacgcagcg
      541 cgtgggaacg aggtaatcgt cgcgcaagaa cgaagaaaat tagtgctagt tcccaagctc
      601 ctgcttggga attcagtacg ggaagctcta gcttcccggc tcgcaaagct agagctttgc
      661 tcactgggtt cccaagcggg agcttgggaa ccagcgtaaa aactacgagt gatagcttgg
      721 agtgcaggct tcgcctgcaa gcgcaagcga agcttgcact cccgttgctt gcgcataaag
      781 atacgtagag cgtggaaacg agcaatcgtt cagttggagc gaagaaaacc agccggtgtg
      841 acgacttcaa tcccgtttgc cgcgccgagt accggagctt tcgccgggat cagcccgcag
      901 gggcgcggca ggaagccgcg cgttgccgga gggccaggga tggcccttcc ggcacccccc
      961 ggcgaaagcg aggaacgcag gaactaagcg gcaaccgggc cgcctttctt ttggatactt
     1021 ttctttgagg gtgtagacaa aatattttta actaattgat tttaaagtgc actttagaaa
     1081 atgaataggt aggtacctat atttttatca ttaaaattca atgacttatt tattttgtct
     1141 acaccctctt tggcggagca aagaaaagta tctcgccgtc gggtgcgaga acccgattca
     1201 atcaagcgtc gcgctagcga cgccttattt tgcaagacga cgcagagcgt ctgggactgc
     1261 attcccacgc cggagcgtgg gaacgatgaa aattaccaca tagagctgct ggcattatga
     1321 ttgtagcttt tgtctttgta agagttttta cacgccttgc attcccaaaa acccttccca
     1381 tttccttgga gtcgaattat atcaaggttt ttatcataac aatgttggca gaatggccca
     1441 tctttcagcg tgccatcaat gagccagtag taaggggact cccattgaag catctccttt
     1501 ttagtaagtt gttcttgcaa aacttttaac ttagcgtctt tttccaacaa aatttgttgg
     1561 atttctgtaa cctgaatttt tgcctctgca agagcggaaa taaggtctgc caatttcaat
     1621 tttgattcgg ctttttcaag tgtatcaaca ctatttttaa tcagctttgc aatttcggta
     1681 gcagttttga tgctgctaag cacggttgtt acatccatga ataattcctt atgtgataag
     1741 tcaagaagtg atggttgggg taggtgttac ttagaaataa atatgttagg aattagtgtt
     1801 gttttgttgg taccgttcca ttttttcttc caagtcctgt ctctccctac ggcgatcctt
     1861 ttcggaatat gtatctgttt tcaaccgttt gaacccagta gccagatgat ccaggttctt
     1921 ctcaatcttt tcaagggatt gagctattgc atacaagtgc ggtgtaccga gcgtgccata
     1981 accctcaaat tcctcaaaat atactggtat cttgtcttta tatgacttac ctgcgctgct
     2041 gttatatgta acctctatca tcaagcgcgc cttgattttt tctgagtaat tctcagttat
     2101 ctcagtgtaa cgagtctgta attcctgccc aggtccgaga taccgtaatc cagtcataag
     2161 aaattgactt cttgaaaagt cctggatgag ctctttctct ccatcagtct gagactcacc
     2221 ttctagtcga aaacacacgt ctaaggcagg tccaagacca atatttttca cgcggagcgt
     2281 aatgatgtta acaaactctt ctttaagaaa ggctattact tcaattttcg gttttgtctg
     2341 taattcacgc atatgcttag tttcttttac taggctatgg gtgagaaacg catacaccac
     2401 tgtagctaac ataacgattc cagagaatat cgtggaaaaa accccttcat tagtgttgag
     2461 aaagcacaga aattcagcaa ttgtcatcta gtatctccta agcattgatg atcaagcccc
     2521 ccatcaccca cacttactct ccccacagtt taagcacgtc atacacccat ccatcaacac
     2581 catagcctta gtactacact tgttacaaag caccgctttc tcaggaaacg cctgcccctc
     2641 cgactcccca tgctgcgttt caaactcctt gcgcttttca tcgagaaact gcttctga
//
LOCUS       DATBTE010000376        13075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_324683, whole genome shotgun sequence.
ACCESSION   DATBTE010000376 DATBTE010000000
VERSION     DATBTE010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 13075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13075
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324683"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..925)
                     /locus_tag="VIG33_14145"
     CDS             complement(188..925)
                     /locus_tag="VIG33_14145"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEY8272028.1"
                     /translation="MNLAHFKKDFKKLSRPHDAKILQRFFKTGPGEYGEGDLFAGIKT
                     SPCRTLAKKYLDLSFADIYLLLSSKIHEERSIALMILVLRFKTALKDADERTQQQIYR
                     FYMKNLKHVNNWDLVDGSAPYINGPYLFKRDRKILYKLATSKVLWERRIAMLTTFHFI
                     RLQDFRDALKLANILLQDKHDLMHKAVGWMLREIGKRDIEAEKAFLDKHAVTMPRTAL
                     RYAIERFPEKERQYYLHLGTIRQRPFV"
     gene            1061..1594
                     /locus_tag="VIG33_14150"
     CDS             1061..1594
                     /locus_tag="VIG33_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272029.1"
                     /translation="MNKTIYLSFSLLLGFSLQASAAIPAKPLVPVVGKILINEGEATG
                     GVAGTGFSLMDLRRTSDVKKKVERIVIDFGDMQGNPQKGLPAYFHAELKKNPNRLVLD
                     FNQTPNSRIDDGRLATRFKDSLFVKKSSMILDPEDKALNLTLDLKPGTFARVFQVKGE
                     KTTAKVVIDLFETKKTK"
     gene            1603..2340
                     /locus_tag="VIG33_14155"
     CDS             1603..2340
                     /locus_tag="VIG33_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272030.1"
                     /translation="MKFAFLALSLCGAMTRAEARVFNINNERLASYFLATGGPSAVKQ
                     NAFLNEANTTNTFTGQVAYNYTGEFGFLYSTPSLSLRFGFEIFSPSSVKDAPANDGSA
                     DIYNLTSDITVYAPKVGLEINLQRTATYRSFITAYTGLGNASYKNEYTIISYPGVTDH
                     TVETKGTAMLYGGSVGVESFMTDTTTYIFEFGYRVMKINNFKYSKDVTTFSGAKVAGD
                     PVLDADGLPRTLDFNGGYVSIGFRFYM"
     gene            complement(2354..4156)
                     /locus_tag="VIG33_14160"
     CDS             complement(2354..4156)
                     /locus_tag="VIG33_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY8272031.1"
                     /translation="MKQKITAHSTATIGNSLLSFKDRTAKKAVMIKRGSQWTSKNWSE
                     YYKDIETLGAGFLALGIKPQDRVAIMSNTRYEWSVADHAAMGIHGIVVSIYQNNTPAD
                     VEYILNNSEAKVLVLENDAVLRVWNEIKSKCPKVKTVILIENKKPIEDPMILSWKDLV
                     QKGEELLQKKPQAFESLCGSLKTTDTATLLYTSGTTGAPKGVVITHAQVISEVGETFP
                     MFGVDERDIALSFLPYAHILGRIEHWAHAFMGFSLAYAESIERIRGNLLEIEPTFLIS
                     VPRIFEKIYDSVGTQLEASPLKKKLFTWAIGVGKKITELKLTRQAVPLDQLAAYELAR
                     RLVLNKISSAFGRRLRFAVSGGAPLAQDISIFFHAAGVLILEGYGLTETTAAITVNTP
                     FNYNFGSVGRPIGDVELKIAEDGEILVRSQKVMKEYYKNPEATAEVMTEGWFHTGDIG
                     TILQGGDLKITDRKKDLIKTAGGKYVAPQKLEGLLKLNPLISQALICGDQKKYIVALL
                     TVDPIYLQNLASARGENATHWTELINRPWVQDFIREYIAETNAGLGNFESIKRFFILP
                     VEFTVENGDLTPSLKLKRKLLSERYKKEIDSLYN"
     gene            complement(4160..6169)
                     /locus_tag="VIG33_14165"
     CDS             complement(4160..6169)
                     /locus_tag="VIG33_14165"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY8272032.1"
                     /translation="MSQQLEQFGKYLLLEKLATGGMAEVYLAKSIGAVGVNKFVAIKR
                     ILPQHSESPEYIEMFKEEAKIAVHLNHGNVVSIYDFGVEYRQFYLVMEYVEGRNLRQI
                     VNEMKKANVQFSIDQIVYIIKEVAAGLDHAHRCIDGGTGRPLNITHRDMSPQNIMVSF
                     EGEVKIIDFGIAKAETQSEATKAGTLKGKFGYMSPEQSDGMPIDLRTDIFSLGIVLWE
                     LLANDRLFTSSSEAAILRKIRECQIPSIHKINPSVPPELERIVNKSLTKDKSLRYQTA
                     AALHRDLNRFLNVQYPEFTPHDFSVFVKTAFSQIYLEQKRRLVEYAQLQLQTQDDKTL
                     VTRMVGNKTEIVAHIAQIPHQVPNTSSIVTGDKSLTMEDNELGINVSDTDLKINLASL
                     KSSADTTSPRKDFYKMEDEKVRHTSPMNTRFTTNSYTSTKVKTAAHRALIIEQAISWG
                     LASIIAVGMIFGGWYFYNTPSGQKMIHNFQTGSEKILVGNTNSSNPTTSKPIEPSPQK
                     TESASIAQYTVLLESQPIGARIYINGEDTGAFTPNRRSLEAYKDYVITLKKEGYLPYE
                     KTIRPVQNASTFSMSMQPAQKAAYIAITVANGGLKTVIYVNGILLGEKPPISKYAIPA
                     GIPVKVTAYDPFNKLSAEQVVKVDANQKKSVDLILGVDPRKPAGQ"
     gene            6209..6841
                     /gene="coaE"
                     /locus_tag="VIG33_14170"
     CDS             6209..6841
                     /gene="coaE"
                     /locus_tag="VIG33_14170"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY8272033.1"
                     /translation="MKTLKESGPLMMKWIGITGGIATGKSTVTQKLRALGYDVLDADE
                     VSHQVTGPGGAALPEIFKTFGDQVKTPEGGLDRKALAALVFGQEEARRQLESIIHPLV
                     GRRVAAERLRLEEQGKSVAFYDVPLLYEKKMEDHFDEVIVVATDEDLQLQRLMKRNSL
                     TEPEAKARLQAQIPIREKARRTKYVIHNTKGPDFLETEILRILRELKLIN"
     gene            complement(6864..7421)
                     /locus_tag="VIG33_14175"
     CDS             complement(6864..7421)
                     /locus_tag="VIG33_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272034.1"
                     /translation="MRLSIPILFLTLVVNSIAANAAPTIHHEPRPIEISPKDKSEPLY
                     SSDPKERYWSFRLGFLGGTLSEAQPTDPLFFYGLGYKFLNGSLSTWQMEVSGAKDNFL
                     HLVVGKKFYFSFQDTLPYYKFSAGDLIDSGGGLGSILNLKKIQAIAAIGLDDVLKWNQ
                     YLQAEIGVSYALIGTQLEISLGFGF"
     gene            7476..8663
                     /locus_tag="VIG33_14180"
     CDS             7476..8663
                     /locus_tag="VIG33_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272035.1"
                     /translation="MVAGRGKLLLTVLVSIVTGPFCFADDSGLSFRIHHHYQSARALG
                     MGDAFVAVANDYSAIFYNPAGLARRTDGQVNLSFDLGATAKFAELSKQITTAQNTPGA
                     EAEKQQAIMDVINGLGGNTYGLRVVGPNGVWVRPNWGIAVIPADVTVQMDVHNQVGPA
                     INTTVYADTTLAYAYARDFDWIPNSRFSLGFTGKIVNRGYISKPVSAMEAAVDPNLVK
                     STDLQEGYTVDADIGTLWTPNIPTEGLLSWLALAKPTFGAVVRNVGEVGFGQSLKLIN
                     KQKTAAPEKLYRVLDLGTRWEYPSLWIFGGRGTLDIRDIGHPLFNLKKGLHAGFEFDW
                     TVTSWWKGHYNVGVSQGYLTAGLGAEFAWFNLDLVTYSEDVGPNGSPQESRSYLARFN
                     MDF"
     gene            complement(8675..9403)
                     /locus_tag="VIG33_14185"
     CDS             complement(8675..9403)
                     /locus_tag="VIG33_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272036.1"
                     /translation="MTYGKKSLSCLYTLALSASFLISCAPKAADKIGDAQLCLDKATQ
                     GTAAACLEKIEGIDTAAANVMRCSAGFIDEGFTDPTRFKKAFDALSQKSAGATNTLTF
                     LSFIAFSSKGTATENKAFATDTYNSCLKTKAKGLTLLGSMAMTATVISGGLTALNDGA
                     AIQTAITSLLGSADDSSKTAVGSAVIATYTSSCTDGSEANQGLCDQLNGYLGTDGAVD
                     ISDPLAVGNAILTNWNGLTPAPVP"
     gene            9672..10757
                     /locus_tag="VIG33_14190"
     CDS             9672..10757
                     /locus_tag="VIG33_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272037.1"
                     /translation="MKRILAVSYLLLLLSFFSPLVGAKERRAFYEGARGRAMGGVQIA
                     TVNDETALLINPAGLGKLREFYGTTFDPEVDYSNNFNKMNSASSISQPFSIAAIKDSL
                     DASRETYYHAKAQMFPSLVAKNFGIGIYGSYLLDGEMNAAGTLMDVYYRSDLALVLGY
                     NFRFFDGRIKFGFNAKLLNRIEVNNSAADPAALDLTTLGVTEGTGISNDVGLIMTAPW
                     ALLPTISAVVHDVGNTTFDKSSGARLQTTDHPAMVSQDMDVALAIFPIHSSHVRSTWS
                     VEYRGLLTSSQENDKAKLLHVGTEFNISDILFIRAGYNQQRYYTAGFEISSEKMQFQF
                     ATYGEEIGDEVTPREDRRTVLKFAFRF"
     gene            10776..11609
                     /locus_tag="VIG33_14195"
     CDS             10776..11609
                     /locus_tag="VIG33_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272038.1"
                     /translation="MKRNIFKIVQVLIVTAGILSCTGSNLYKDLASNKTTDEALYEDA
                     QKLIDSADYTGAITKILATTADFQATAKVKESLAGAYAARCGMEFIPFVTNLTGGSSS
                     SFFQMAMNGFVGVSTANYADCVTAESIIESIGVIGVRSQSENLFLLVLEMAKIGNRLR
                     ADADILPTDAGDGTVDAGFSCSTSVPIADAKAIIESFYKFLSLFAVVGSTLSGVSGIQ
                     SFLDSFGGSLPALDYSGGLDASEDEADPPILLSRAMINSQSFGVGSCNNADPTQCVCP
                     P"
     gene            11606..12712
                     /locus_tag="VIG33_14200"
     CDS             11606..12712
                     /locus_tag="VIG33_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272039.1"
                     /translation="MKTLFGFFLFIFSSLSSHAFEVMETHHNVRSNGMGGIYMSIVND
                     SNALFVNPAALGRTEGLNLEVLNLEVGINGLDVYNDFKDINSSDSASFSEFFGKDIWL
                     RSGGKAAITLPYFGIGAYTDIQTHLVLHNPAFPQFETTFLNYYGLVAGGAFPLGPISY
                     GGLSFKRITRWGGQQNIDLGVIAAGDPAQIADQFKNKGNGYGVDMAFMTMLPMPLSPS
                     LSLVWQDVGSTAFTKSDGIDAPPRIHDNLSFGASTLMDLPGLDWTTGFEYRHITESQY
                     HFGQKLHMGTEISLPFIDLRAGLNQGNASYGLGLSFLFLHFDVASYTEEIGAYPGQTS
                     QKRIELGLSLDLSFDADFSITNSHGKKRKLKQRR"
BASE COUNT         3378 a         2791 c         3177 g         3729 t
ORIGIN      
        1 tgacgcctat gaaaatttaa ttttttaccg gacagcagtg agaattttga actggggtcg
       61 ggacttgaga aacgtctttg attattgcga actcaatgag cttgaaggct ggggtttgtt
      121 tcgcgaaaaa aaagtctagt aacactttcc cagagcaggc tgctctctgc gccgagccct
      181 ctacctccta gacgaagggc ctttggcgaa tagttcctag atgaaggtag tactggcgct
      241 ccttttcagg aaatctctca atggcatagc ggagagccgt gcgaggcatg gtgaccgcgt
      301 gcttatccaa aaaggccttt tccgcctcga tgtcgcgctt gccgatttcc cggagcatcc
      361 agcccactgc cttatgcatg agatcgtgtt tgtcctggag aagaatattc gcgagcttta
      421 aggcatcgcg gaaatcctgc agtcgaataa agtgaaaagt cgtgagcatg gcgattcgcc
      481 gttcccaaag aacttttgaa gtcgccaatt tataaagaat tttacggtcc cgtttaaaca
      541 agtaaggacc attgatataa ggggcggagc catccaccag atcccaatta tttacatgtt
      601 ttagattctt catataaaac ctataaattt gttgttgagt gcgctcatcc gcatctttga
      661 gagctgtctt aaagcgcaaa accaagatca ttagggcgat gctgcgctct tcatggatct
      721 tggaactcaa aaggagataa atatctgcga aagaaaggtc gaggtatttt ttcgcaaggg
      781 ttcggcaggg ggaagtttta atcccagcaa agagatctcc ctcgccatat tccccaggac
      841 ctgttttaaa aaagcgctgc aggatttttg cgtcatgagg acggctcagt ttcttaaagt
      901 cctttttgaa gtgagccaag ttcataacaa ctccgattta tatcttcaga ctatattcgc
      961 aaactcgaaa aataaaatct actaaaaagt ccgagctcag ttgacccgca ctaaggatcg
     1021 ggtgaaactt aagagtgtcc tctgaaaagg agttctaagg atgaataaaa ctatctatct
     1081 ttctttcagt ctacttttag gtttttcctt gcaagcttcg gcagcaattc ctgcaaaacc
     1141 tctcgtacct gtcgtcggta aaattctcat caatgagggt gaagcgaccg ggggagtggc
     1201 ggggacgggt tttagtttaa tggatttacg gcgcacttcc gatgttaaaa agaaagtgga
     1261 gcgtatcgtg attgattttg gcgatatgca ggggaatcca caaaaaggac ttccagctta
     1321 ttttcatgcg gaattgaaga agaatcccaa tcgattggtt ttagatttca atcaaacacc
     1381 caacagccgc atcgacgatg gacgtttggc aactcggttc aaagattcct tatttgttaa
     1441 aaagagctcc atgattttgg accccgagga caaggccttg aatctaacct tggatttgaa
     1501 acctggaact tttgcccgtg tcttccaagt gaaaggcgaa aaaaccactg cgaaagtagt
     1561 gattgacctt ttcgagacta aaaaaaccaa atagaggtgg ctttgaagtt tgcatttctg
     1621 gcgctttctt tatgtggtgc gatgactcgg gctgaagccc gggttttcaa tattaataat
     1681 gaaagattag ccagctattt tcttgcgacc ggaggtcctt cggcggtcaa acaaaatgct
     1741 tttctgaatg aagcgaacac caccaatacc tttaccggcc aagtggctta caattatacc
     1801 ggtgagttcg gctttcttta ttccactccc agcttgagtc tgcgctttgg gttcgagatc
     1861 tttagtcctt cttctgtgaa ggatgctcca gcaaatgatg ggagcgccga tatttataat
     1921 ctgaccagcg atataacggt ctatgcgccg aaagtcggct tggagatcaa tctccagaga
     1981 accgctacct atcgatcttt tatcaccgct tataccggct tgggcaatgc tagttataag
     2041 aatgaatata caattatcag ttatcccggc gtgacggacc atacggtcga aaccaaaggc
     2101 acggccatgc tttatggggg ctccgtcggt gtggaatctt ttatgaccga taccacgact
     2161 tatattttcg aattcggcta tcgagtgatg aaaattaata atttcaaata cagtaaagac
     2221 gtgaccacat ttagcggggc aaaggtcgcg ggcgatccgg ttctcgatgc ggatggcctg
     2281 ccacgaactc tcgattttaa cggaggatat gtgagcattg gcttccgctt ttatatgtag
     2341 tcttatttta attttaatta tacagactat cgatttcttt tttatatctt tcgctgagca
     2401 gcttgcgctt gagtttcaag gacggagtca ggtctccgtt ttcgaccgtg aactccaccg
     2461 gaagaatgaa gaagcgttta atactttcga aatttccaag ccccgcattg gtttcagcga
     2521 tatactcgcg aatgaaatct tgcacccacg gacgattgat gagttcagtc caatgggtgg
     2581 cgttttcgcc gcgggcactg gcgaggttct gtaaataaat gggatccaca gtcagcaggg
     2641 cgacaatgta ttttttttga tctccgcaaa tcagcgcttg ggaaatcaat ggatttaatt
     2701 ttaaaagacc ttcaagcttt tgtggcgcca catatttgcc gccggcagtt ttgataagat
     2761 cttttttgcg atccgtgatt ttaagatctc ccccttgcaa aatcgtgccg atgtcgcccg
     2821 tatggaacca accctcggtc ataacttcgg cggtggcttc gggattttta taatattctt
     2881 tcatgacttt ttgactgcga acgagaatct caccatcttc agcgatttta agctccacat
     2941 caccgatcgg gcgcccgaca gagccaaagt tatagttaaa cggagtattt accgtgatgg
     3001 ccgccgtggt ttctgtgagg ccgtaacctt ctaatatcaa aacgccggcg gcgtgaaaga
     3061 aaatagaaat atcttgggcc aggggggctc cgccactcac tgcaaaacgc agacggcgac
     3121 cgaaggcaga actgatttta ttcaggacca gtcttcgggc caattcgtaa gccgccagct
     3181 gatccagcgg aaccgcttga cgagtgagct tgagttcagt gatttttttg cccactccga
     3241 tagcccaggt gaaaagtttt ttcttcagag gtgaggcctc gagctgggtg cccacagaat
     3301 cgtagatttt ttcaaaaata cgcgggaccg agattaagaa agtaggctcg atctccagca
     3361 aatttccacg gatcctctca atgctttcgg cataagcaag agagaagccc ataaaggcgt
     3421 gggcccaatg ctcaatacgt cccaaaatgt gggcgtaggg cagaaagctc agtgcgatat
     3481 ctcgctcatc aacgccaaac atagggaagg tctcgccgac ttctgaaatg acttgagcat
     3541 gggtgatcac cactccctta ggggcgcctg tggtgccgga agtataaaga agggtggcgg
     3601 tgtcggtcgt tttcagggag ccacaaagac tttcgaaggc ttgaggtttt ttttgtaaaa
     3661 gctcctcgcc tttttgcacg agatctttcc agctcagaat catggggtct tcaatcggct
     3721 ttttattttc gataagaatc acggtcttta cttttggaca cttggatttg atctcattcc
     3781 aaactctcaa tacagcgtcg ttctctagaa cgagaacctt ggcttcagag ttgtttaaaa
     3841 tgtattccac atcagccgga gtgttgtttt gataaattga aacgacgatc ccatgaattc
     3901 ccatagccgc gtggtcggcg acggaccact catagcgagt gttggacatg atcgcgacgc
     3961 gatcctgggg ttttatacct agagctaaga atccagcccc tagagtttca atatctttat
     4021 agtattcaga ccaatttttg ctggtccatt gagagccccg tttaatcatc actgcttttt
     4081 tggcagttcg atctttaaac gaaagtaaac tgtttccgat agtggcagtg gagtgcgcgg
     4141 tgattttttg cttcataaat tactgtcctg caggctttct tgggtcgacg ccgagaataa
     4201 gatcgacgga ttttttctgg ttggcatcga ccttgaccac ttgttcagct gaaagtttat
     4261 taaaggggtc ataagctgtg actttcacag ggatgccagc cgggattgcg tatttactaa
     4321 taggcggttt ttcacctaag agaattccat tgacgtagat taccgttttt aagcctccgt
     4381 tggcaactgt gattgctata taagcggctt tttgagcagg ctgcatagac atagagaacg
     4441 tggatgcatt ttgtacaggc cggatcgtct tctcatatgg taaataaccc tcttttttaa
     4501 gcgtgatcac gtaatcttta taggcttcca aagagcgacg gttcggggtg aaggccccag
     4561 tatcctctcc attaatatag atacgggctc caatcggttg actctctaaa agcactgtgt
     4621 actgcgcaat agaggcggat tcggtttttt gcggactggg ttcgatgggt ttcgacgtag
     4681 tcggattact tgagttcgta ttcccaacca atattttttc gctgccggtt tggaaattat
     4741 gaatcatttt ttgacccgac ggagtattat aaaagtacca gcccccgaag atcatgccga
     4801 cggcaatgat ggatgccaat ccccagctga tggcctgttc aataattaag gcccggtgtg
     4861 ccgctgtttt cacctttgtg gaagtataac tattagtggt gaatcgggtg ttcatcggcg
     4921 aagtgtgacg gactttctcg tcctccattt tatagaaatc tttacgcggg gaggtggtgt
     4981 ctgcggagct ttttaagctg gctaaattga tttttaaatc cgtatcggaa acgttaattc
     5041 ctaactcatt gtcttccatt gtcagactct tgtccccggt aacaatggaa gatgtattcg
     5101 gcacttgatg gggaatttgc gcgatgtgcg cgacaatctc agtcttattt ccgaccatgc
     5161 gagtgactaa agttttatcg tcttgggtct gtaattgcag ttgggcatat tccacaagac
     5221 ggcgcttctg ttcgaggtaa atttgtgaaa aagcggtttt cacaaacacg ctgaaatcat
     5281 gcggagtgaa ctctggatat tgcacattca aaaatcgatt cagatcgcga tgaagagcgg
     5341 ccgcggtttg gtagcgaagg cttttgtcct ttgtcagaga tttattcacg atacgctcta
     5401 attcaggggg cacagacgga ttgattttat ggatgctggg aatttggcac tcgcgaattt
     5461 ttcttaaaat cgcggcctca ctgcttgaag tgaaaaggcg gtcattggcc aaaagctccc
     5521 aaagaacgat tcccagagaa aaaatatccg tgcgcaaatc gatgggcatg ccatctgatt
     5581 gctcaggact catgtagccg aatttacctt taagagttcc cgctttggtg gcttccgatt
     5641 gggtttccgc tttagcgatc ccgaagtcga tgatttttac ttcgccttca aaactgacca
     5701 tgatgttttg cggactcata tcacgatggg tgatattcag cgggcgtccc gtgcctccat
     5761 caatacaacg atgagcatga tccagaccgg ccgccacttc tttgatgatg tagacgattt
     5821 gatcgatcga gaactgcaca ttggcttttt tcatttcgtt gacgatttga cggagattgc
     5881 gaccttcgac atattccatc acgagataga attgacgata ctccacgccg aaatcgtaga
     5941 tggataccac attgccgtga tttaaatgca cggcaatttt agcttcttct ttgaacattt
     6001 caatgtattc ggggctttca gaatgctgag gaagaatgcg tttgatggcg acgaatttat
     6061 taactcccac agcaccgatg gactttgcca ggtacacctc ggccattccg ccagtggcaa
     6121 gcttttcgag gagtaggtat ttaccaaact gttccaactg ctgcgacatt aaattcgttt
     6181 cccctttgtt gaaaatatcg gtaaaataat gaaaacgctt aaggaatctg gacctttaat
     6241 gatgaaatgg attgggatca ccggtggaat cgccaccgga aaaagtacag tcactcaaaa
     6301 gctcagggct ttaggctatg atgttttgga tgcggacgag gtctcccacc aggtcacagg
     6361 tccaggcggg gcggcgctgc cggagatttt taaaactttt ggtgatcaag tgaagactcc
     6421 agaaggtggt ttggatcgaa aagctcttgc cgctctcgtc tttggtcagg aagaggcgcg
     6481 aagacagttg gaatccatta tccatccttt agtcggtcgt cgagtggctg cggaaagatt
     6541 gcgactggaa gaacagggca aaagcgtcgc tttttatgat gtccccttgt tgtacgaaaa
     6601 aaaaatggaa gatcattttg atgaagtgat cgttgtggct acggacgaag atttgcaatt
     6661 acaaagactt atgaaaagaa attcactgac agagcctgaa gctaaagctc gtttacaggc
     6721 acagatcccc attcgagaaa aagctaggag aacaaaatat gttattcaca acaccaaagg
     6781 tccagatttt ttggaaacgg aaattctgcg cattctccgg gagctaaagc taatcaacta
     6841 aagttctgac ctaaaagatt tatttagaag ccaaaaccaa gggatatttc gagctgagtg
     6901 ccaattaagg catagctcac gccgatttca gcttgaaggt actggttcca tttcagtaca
     6961 tcatccaaac ctatggcggc tatggcttgg attttcttta aattcaagat ggagccgagt
     7021 cctccccctg aatctataag gtcgcccgca ctgaatttat agtaaggcaa ggtgtcttga
     7081 aaagaaaaat agaatttctt gccgacgacg aggtgtagga agttatcttt agcgccactg
     7141 acttccattt gccaggtgct gagggaccca ttcaggaact tgtaacccaa gccataaaaa
     7201 aataagggat ctgttggctg ggcttcgctc agggttcctc cgagaaaacc cagtcggaaa
     7261 gaccaatagc gctcttttgg gtcagagctg taaagaggtt cacttttatc tttgggtgag
     7321 atttcaatgg ggcggggctc gtggtgaatg gttggagccg catttgccgc gatgctgttc
     7381 acgaccaaag tcaaaaaaag aataggaatg gaaagacgca tacaagaaag tatattttac
     7441 gaatacactt ttgggaaagt gttattggag gaattatggt ggctgggaga ggcaagcttc
     7501 ttttaacggt cctggtaagt atagtgactg gacctttttg ttttgcggac gatagtggat
     7561 tgagttttcg catccatcat cactatcaat cagcccgcgc tcttgggatg ggagatgctt
     7621 ttgtcgccgt cgctaatgat tacagtgcaa ttttttataa tccagccggt ttggctcgtc
     7681 gtacggatgg gcaagtcaat ttgtctttcg atttgggggc cactgcaaaa ttcgccgagc
     7741 tctcaaagca aatcactacg gctcaaaaca cccctggagc tgaggcggaa aagcagcaag
     7801 cgattatgga cgtaattaac ggactgggtg gcaataccta tggacttcgt gtcgtcggac
     7861 cgaatggagt ttgggttcga ccgaactggg ggatcgcagt gattcccgcg gatgtgacgg
     7921 tgcaaatgga tgtgcataat caggtgggtc ccgctattaa tacgactgtt tacgcggata
     7981 cgaccttggc ttacgcatat gctcgggatt ttgactggat acccaattcc agattttctc
     8041 tgggcttcac cggtaagatt gtcaatcggg gttatatcag taaaccggtg agcgccatgg
     8101 aagccgcggt ggatcctaac cttgtgaagt caacggatct gcaagagggt tatactgtgg
     8161 atgcagatat tggaaccctg tggactccca atattcctac tgagggattg ctttcttggt
     8221 tggctctggc caaacccact ttcggggcgg ttgttcgtaa tgtcggcgag gtgggttttg
     8281 gccaatcgct gaagttgatt aataagcaga agacggcggc acctgagaaa ttgtatcgcg
     8341 ttttagatct gggcactcgc tgggagtatc cttcactttg gattttcggg gggcgtggca
     8401 ctttggatat tcgtgatatc ggacatcctc ttttcaatct caaaaaaggt ctgcatgccg
     8461 gcttcgagtt tgactggaca gttactagct ggtggaaggg acattacaac gtcggagtca
     8521 gccaaggcta tctgactgca gggctcgggg ctgagttcgc gtggttcaat ctggacttag
     8581 tgacctattc tgaagatgtt gggccgaatg gttctcctca agaaagccgc agctacctcg
     8641 cacgcttcaa tatggatttt taatttttta ccgcttaagg aaccggtgcc ggagtgaggc
     8701 cgttccaatt agtaagaatg gcattcccca cagcgagagg atctgaaata tccacagctc
     8761 catcagtacc caggtacccg ttcaattgat cacagagccc ctggttggct tcagagccat
     8821 cggtgcagct agaggtgtat gtcgcaatca ccgcacttcc tacggcggtt ttagaggaat
     8881 catccgcact gccgagtaaa gaagtgatgg cagtttgaat ggcagcgcca tcattaaggg
     8941 ctgttaatcc tccggaaatg accgtcgcgg tcatcgccat agatcctagc agagttagtc
     9001 cctttgcctt ggtttttaga caagaattat aagtatcggt tgcgaaggcc ttgttttccg
     9061 tggctgtacc tttgctgcta aaagcgataa atgaaaggaa ggtcagggta ttggtggcac
     9121 cggcggattt ttggctaaga gcatcgaaag ctttcttgaa gcgagtagga tcggtgaaac
     9181 cctcatcaat aaagcccgca gagcagcgca tgacattggc agccgcagta tcaattcctt
     9241 caattttttc caagcaagct gccgcagttc cttgggtggc tttatccaag cacaattggg
     9301 catcaccgat tttgtcagca gcttttggag cacaagaaat taaaaaagaa gcactcaaag
     9361 ccagtgtgta taaacaagat aaacttttct ttccataagt cattgaaata tcctccgcag
     9421 atcctcaccg ttatatctct actattatat tattctacgg cagaaagtga acgggggtta
     9481 aatttctgga tcttttttat tttctgggta aatcaccccc gtttgattta aaaatgaaat
     9541 tcttaatttg ggatttataa tttggaagag tctcacttct ggacctatgg acggatttct
     9601 cttcaatgtc tcaacctgag agctcactct gccgataaga acgctaggac agtgaagtct
     9661 gaggaattta aatgaaaagg attttagcgg tttcatatct actactcttg ctttcctttt
     9721 tctcccccct tgtgggagcg aaagaacgtc gtgcttttta tgaaggcgcg cgcgggcgag
     9781 ctatgggggg agtgcagatc gcgactgtca acgatgaaac ggctcttctc attaatcccg
     9841 caggcttggg taaattgcgc gaattttatg gaactacatt cgatcctgaa gtggattatt
     9901 caaacaattt caataaaatg aattccgcct cttccatctc tcaacctttt agcatcgccg
     9961 ctattaaaga ctcactggat gcctctcgag aaacttatta tcacgccaaa gctcaaatgt
    10021 ttccttccct ggtggcgaag aattttggca tcggtattta tgggagctat cttttagacg
    10081 gcgagatgaa cgctgcgggg actctcatgg atgtctacta ccgaagtgat ttagctttag
    10141 tgttaggata caacttccgt ttcttcgatg gtcgaatcaa atttggcttt aatgcgaaac
    10201 tgctcaaccg tattgaagtg aacaattctg cggccgatcc ggccgctttg gatttaacga
    10261 ccttaggggt taccgaagga acaggcataa gcaatgatgt gggtttaatc atgacggccc
    10321 cttgggcgtt gttgcccacg atcagtgcgg ttgtgcatga tgtgggaaat acgacttttg
    10381 acaaatccag tggggcccgt ctgcagacga cagatcatcc cgcgatggtc tcacaggata
    10441 tggatgttgc tctcgcgatt tttcctattc attcctctca tgtgcgctcc acatggtcgg
    10501 tggaataccg tggtttatta acatcatctc aagaaaacga taaagcgaaa ctgcttcatg
    10561 ttggaacgga atttaatatt tcggacatcc tttttatccg ggccggttac aatcaacaaa
    10621 ggtattacac agcaggtttc gaaatttctt ctgagaaaat gcaatttcaa tttgcgacat
    10681 acggagaaga aataggtgac gaggtcacac caagggaaga ccgaagaaca gtgcttaagt
    10741 ttgcatttcg attctaagcg gaggggatcg gaagtatgaa aagaaatata tttaagatag
    10801 tccaagtctt gatcgttacg gcaggaattc tttcttgcac gggatctaat ttatataaag
    10861 acttagcttc gaataaaacc accgatgaag ctctgtatga agacgcgcaa aaactgattg
    10921 attccgcgga ttatactgga gcgattacaa agatccttgc cacgacggcg gattttcaag
    10981 caacggctaa agtaaaagaa agtctggcgg gagcctatgc cgctcgttgc gggatggaat
    11041 ttattccctt tgtgactaat ttaaccgggg gctcctcgag ttcttttttt cagatggcga
    11101 tgaatggatt tgtaggagtc agtacggcca actatgccga ttgcgtaact gcggaaagca
    11161 tcattgaaag tattggagtt attggagtta gatctcaaag cgagaatcta tttttattgg
    11221 ttttagagat ggcgaaaatc ggcaatagac tccgagccga tgcggatatt ttgcccaccg
    11281 acgctgggga tggaactgtg gatgctggat ttagttgtag caccagcgtt cccattgctg
    11341 acgccaaagc aatcatcgaa agcttttata aatttctgag cctttttgcg gtggtgggct
    11401 caactcttag tggggtttcg gggattcaaa gctttcttga tagctttggg ggttctttgc
    11461 cggcgttgga ctattcagga ggcctagacg cgagcgagga tgaagccgat cctccgattt
    11521 tattgtcaag agcgatgatc aattcacaat ctttcggcgt tggaagttgt aataacgccg
    11581 atcccacaca gtgcgtgtgc ccgccatgaa aacacttttt ggtttttttc tttttatttt
    11641 tagttctttg tcttcccatg ctttcgaagt gatggagact catcataatg ttcgatccaa
    11701 tggcatggga ggcatttaca tgtcgatcgt gaatgattcc aatgctcttt ttgtaaaccc
    11761 cgcagccctg ggacgaacgg aaggacttaa cttagaggtt ctgaaccttg aagtggggat
    11821 caatggttta gacgtctata acgattttaa agatatcaat tccagcgatt cagccagttt
    11881 cagcgagttc tttggaaaag atatttggct tcgcagcggg ggaaaagcgg cgatcacttt
    11941 gccctacttt ggaattggcg cttataccga tattcagacg catctggtgc tccataaccc
    12001 ggcttttcca caatttgaaa caactttttt aaattactat ggattggtgg cgggaggggc
    12061 ttttcccttg gggccgattt cttacggtgg tttgagtttt aagcgcatta cgcgttgggg
    12121 tggccagcaa aacattgatt taggggtgat tgccgcaggc gatcctgcac aaattgcgga
    12181 tcaatttaaa aacaaaggca atggttacgg tgtggatatg gcattcatga ccatgctccc
    12241 gatgccgctg agcccatcgc tttctttggt gtggcaggat gtgggatcga cggcctttac
    12301 aaaatcggat ggaatcgatg cacccccacg aattcatgat aatttaagtt ttggtgcttc
    12361 tactttgatg gatttgccgg gccttgattg gacgacgggc tttgagtatc gtcatattac
    12421 tgagagccaa tatcatttcg gtcaaaagtt acatatggga actgaaattt ctctgccttt
    12481 cattgatctc cgtgcgggtc ttaatcaagg caatgcgagc tatggattgg gacttagctt
    12541 tttattttta cattttgatg tggcaagtta taccgaagag atcggggctt atcctggtca
    12601 aacctcacaa aagcgcattg aacttggttt gagcctggat cttagctttg atgccgattt
    12661 tagcattaca aattctcacg gtaaaaaacg aaagttgaaa cagcgtcgtt agttaagggg
    12721 tttttcaggc aagagccgat tgctctcctc ataagtcgcg aaggcgttta ttattcattt
    12781 tgaaactcgt agcgcgactt tacttctgtt tgagtacaac cagaagttca atgaaaacat
    12841 tcggatagaa taaagacttg ttttaataaa aaccaatgga tcttggtctg gcatattttc
    12901 tatcatcagt tggaacaaac tacccccttc taaaaaacct tatctttacc cacaattaaa
    12961 agcttcaatt gacctccatg ttttttccga ttgcatcagc accatccatc ctctccaaac
    13021 agagatcatt cctggtggac cgtcattatt tcgtcctata tagcccccta gtttt
//
LOCUS       DATBTE010000377         2425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_187542, whole genome shotgun sequence.
ACCESSION   DATBTE010000377 DATBTE010000000
VERSION     DATBTE010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187542"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VIG33_14205"
     CDS             complement(<1..835)
                     /locus_tag="VIG33_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272040.1"
                     /translation="MKLRLVKSLVVASSFLALVPASHAAELCAMVFEQSKDLARSPAF
                     PQDPITLSRVRAEVENSKVASDFFMSEFQKQSDSLKAKYKNKVLELDYLCIGAGPQCA
                     AASLVLGGVKLRTLVLEKTDLVSKTFAEKDFFINSTESDGLSMHQFPNGVGDLADYTS
                     QKYALSSQLAAHLQLQQFASNIPVLLETTMTHVRVLKKEGKPLLEITTDQGIVITAKN
                     LLLGTGLGEIGTKVSDQNYQAAFKANYQKHLTQPDAIQPMMSTDTFLSVVKKASGSGK
                     NI"
     gene            complement(1052..>2425)
                     /locus_tag="VIG33_14210"
     CDS             complement(1052..>2425)
                     /locus_tag="VIG33_14210"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEY8272041.1"
                     /translation="EFSIKELFLNQASWQKQRSSSLLNSGLGWLEKLSRSEAAPLHSF
                     AEYNWKHLVEKEIIPHQKPRGSWGGYTLSTLFSLVTIDHYYNHDFSQKREIEQQMKKG
                     FSFLQTLYFNTGSSSLQGVLDDGSYWDTALALRALRENGIANEEIQNSAQFLANLQQK
                     NGAFPFGYDFEDYSDVDDTVETVFALENLNLAHYNEKKALKFVANFQNRDFGWGTFDK
                     NNTGNALLRIFTQNFNDSAEMFDISRPDVTGHALELLGHFGYTSTKSPQVAKSIHYLR
                     LQRDKKTMASWMGRWAVNHIFGTSAVITGLRAVGLPSKDPIIQDAIRWLKSIQNKDGG
                     FGETTESYQDIRLLAANKGVSTASQTGWAIMALVDGGEAQSEATSKAVDFLMKQFREN
                     GQSSGSTGFWTDASVTGTGHPGLIYMIYPSYPYTWPLIALGKYMRAVCKVESHPHQNY
                     CENYLRK"
BASE COUNT          629 a          529 c          567 g          700 t
ORIGIN      
        1 gaatattttt tccactgcca cttgccttct ttacgacact caaaaaggta tctgtagaca
       61 tcatgggttg tatggcgtcg ggttgagtga gatgcttttg ataattagct ttgaatgcgg
      121 cctggtaatt ttgatcactg actttcgtgc cgatttctcc gaggcctgtt ccaagaagga
      181 ggtttttcgc ggtaataacg attccttgat ccgtggtgat ttcaagcagg ggctttccct
      241 cttttttcag cacgcgaaca tgggtcatgg tcgtctccag gaggaccggg atattagaag
      301 caaactgttg caattgaaga tgggcggcta gctgtgaaga caaggcatac ttctgggaag
      361 tataatcggc caggtctccc actccgttgg ggaattgatg catagagagt ccatcagact
      421 ccgtagagtt aatgaaaaaa tctttttcag caaaagtctt ggataccaaa tccgttttct
      481 ctagaacgag ggttcgtaat ttgacgccac ctaatacaag ggaggccgcc gcgcactgtg
      541 gcccggctcc tatgcacaga taatcgagtt ctaaaacttt atttttatat tttgccttga
      601 ggctgtcaga ctgtttttga aactcgctca tgaaaaaatc agaagcgact ttggaattct
      661 ccacttccgc gcggacacgg cttaaagtta tcggatcctg tggaaaagcc ggactgcgag
      721 ctaaatcttt tgattgctca aacaccattg cacagagttc cgcggcatgc gatgcgggaa
      781 ctaaagccaa aaagcttgag gcaacaacaa gagatttcac taagcgcaac ttcacggggt
      841 cctcctttgg acttacctgc attcaatata aaaaagtact gcttagagat tagttgcgaa
      901 aaatatgcca ataaaacaaa cggggagtta gtgcttaact cattggagag agacacaaat
      961 taaggtgttt aaaggttggt cagatgagag caattccgcg ccttggggaa ggcctctccg
     1021 ttagagaact ttgggacatc ggcggcagat tttatttcct aagataattc tcgcagtagt
     1081 tttggtgagg atgggactcc actttgcaga cagctctcat atacttgcct agggcgatta
     1141 atggccatgt atagggataa gaaggataga tcatataaat caatcccggg tggcctgttc
     1201 cggtcacgct tgcatcggtc caaaaaccag tgcttccact gctttgtcca ttttccctaa
     1261 attgtttcat taaaaaatcg acggctttgg aggtggcttc ggattgggct tcgccaccat
     1321 caaccagagc cataattgcc caaccggttt ggctcgctgt gctgacgcct ttattggccg
     1381 ctagcaatct tatatcttga taagactccg tggtttctcc aaaaccccca tctttatttt
     1441 gaatgctctt gagccagcgg atcgcatcct gaataatggg gtccttactg ggcaaaccca
     1501 ccgctctgag gccggtgatg actgcgctgg ttccaaagat atggtttacc gcccatctcc
     1561 ccatccaaga agccatggtt tttttatccc tttgaagtct taagtaatga atgctcttag
     1621 cgacttgagg ggatttggtg gaggtgtagc cgaaatgtcc gaggagttcc aaagcatgtc
     1681 ctgtgacatc ggggcggctg atgtcgaaca tttctgctga atcattgaag ttttgtgtaa
     1741 aaattcttaa gagcgcattg ccggtgttat ttttgtcgaa tgtgccccac ccaaaatctc
     1801 gattctgaaa gttggcgaca aatttcaaag ccttcttttc attgtaatgc gcaagattca
     1861 ggttctctaa ggcaaaaacg gtctctacgg tgtcatctac gtcagagtag tcttcaaaat
     1921 cgtatccgaa aggaaaggct ccattttttt gttgaagatt tgctaaaaat tgcgcggaat
     1981 tctgaatttc ttcattggca attccatttt ccctcagagc ccgcaatgca agcgcagtat
     2041 cccaataact cccgtcatcc agcactccct gaagggaact gctgcctgtg ttgaaataaa
     2101 gagtctgtag aaaagaaaat ccttttttca tttgctgttc gatttctctt ttttgcgaga
     2161 agtcgtggtt atagtaatga tctattgtta ctaagctgaa taaagtggag agtgtatagc
     2221 ctccccaact tccacgaggt ttttgatgag ggatgatttc cttttctact aaatgcttcc
     2281 aattatattc agcaaacgag tgcaggggag cagcctcaga tcgactgagc ttctccagcc
     2341 agccgagccc agagttaagc agggaagagg acctttgttt ttgccacgaa gcctggttaa
     2401 gaaagagttc tttgattgaa aattc
//
LOCUS       DATBTE010000378         1799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_528125, whole genome shotgun sequence.
ACCESSION   DATBTE010000378 DATBTE010000000
VERSION     DATBTE010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528125"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VIG33_14215"
     CDS             <1..224
                     /locus_tag="VIG33_14215"
                     /inference="COORDINATES: protein motif:HMM:NF044283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HEY8272042.1"
                     /translation="MTTRMDPARDLTILENTPIDYLDFASPVSGLGSKVGFDATNKWP
                     GETNREWGRTIVMSPEVVKKVDEMWDMLF"
     gene            297..>1799
                     /locus_tag="VIG33_14220"
     CDS             297..>1799
                     /locus_tag="VIG33_14220"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEY8272043.1"
                     /translation="MAKVNLDALIAREDFEVEEKVNSGKKKETISIEDIKADSFFFPN
                     LKKPDFQRETNEWDSQKICELIRSFIEGDLIPAIILWRSSGGYLFVIDGSHRLSALSA
                     WVNDDYGDGETSKLFYDAIIPDEQLEIAQETRKLVNKTVGSYKDFRLALTHPDKVKPE
                     IVKYAKNLGALAIQLQWVEGDARKAESSFFKINQQAAPIDKTELKLLESRKKPNSISA
                     RAIIRGGKGHKYWSFSEPIQIEVQELAAEINEILFDPKLQTPIKTLDIPVGGKAYSHQ
                     ALPLVLDFINIVCNAKPNDKDDETGETTIQTLKNVRKVAYRLNSNHPSSLGLHPSVYF
                     YSMEGRHKTASFFAMVDFVMALDKQKKINDFVDIRIKFEKFILEYDYLIQQINRKHRT
                     AQKSYKHISSFFQKIVFAFKAGKSVDEAISEIVSSRDFEYLTFQKPSDQSDSLAKDFN
                     TGKKSAIYMRDALEKAPKCHICGGLIHRNSISIDHIQRKQDGGLATLDNGQ"
BASE COUNT          630 a          311 c          370 g          488 t
ORIGIN      
        1 caatgacgac ccgcatggac cccgccaggg atttgaccat actggaaaac acgccgatcg
       61 attatcttga tttcgcctcg ccggtttcag gcttaggctc caaagtggga tttgatgcga
      121 ctaataaatg gccgggagaa actaatcggg aatggggcag aacgattgtt atgtcgccgg
      181 aggtggtcaa gaaagtggat gagatgtggg atatgttgtt ttagtctgta gggtaggtaa
      241 acgataaagc tgtttgccca acatgactaa tgatttagat ttttatagga actaatatgg
      301 caaaagttaa tttggacgct ttgattgcaa gagaagattt tgaggtagaa gaaaaagtaa
      361 attctggtaa gaaaaaagaa accatttcaa ttgaggatat aaaagcagac tcttttttct
      421 tcccaaacct caaaaagcca gactttcaaa gagaaactaa cgaatgggat agccaaaaaa
      481 tttgtgaact tataaggagc tttatcgaag gcgatttgat accagcaatt atcctatgga
      541 gaagctccgg tggatactta ttcgtaattg atggaagcca tagattaagt gctttatctg
      601 cttgggtcaa tgatgactac ggagatggag aaacctcaaa actgttctat gatgccatca
      661 tccccgatga gcaactggag atagcacagg aaacgcgaaa acttgtgaac aagactgtcg
      721 gatcctataa agattttaga ttagctctta cacatccaga taaagtcaaa ccagaaattg
      781 ttaaatatgc taaaaattta ggtgcattag cgatacaact tcagtgggtt gagggcgatg
      841 caagaaaagc agaaagctca tttttcaaga taaatcagca agctgctcca attgataaaa
      901 cagaactaaa gcttcttgaa tctagaaaaa aaccaaacag tatctcagct cgtgcaatca
      961 ttaggggtgg gaaaggtcac aaatattggt cgttttcaga acctatacag attgaagtgc
     1021 aagaactggc tgcagagata aatgaaatcc tgtttgaccc aaagcttcaa acacctatta
     1081 aaactctcga tataccagtg ggaggaaagg cttattctca tcaagcttta ccgttagttt
     1141 tggatttcat aaatattgtt tgcaatgcga aaccaaacga taaagatgat gaaactggcg
     1201 aaacaacaat acaaaccctc aaaaatgtca gaaaagttgc atatagatta aatagcaatc
     1261 atccttcatc gcttggttta catccctctg tgtatttcta ttctatggaa ggaagacaca
     1321 aaacagcttc attctttgct atggttgatt ttgtgatggc gctagacaag caaaaaaaga
     1381 taaatgattt tgttgatatt cgaattaagt tcgaaaaatt tatccttgaa tatgactatc
     1441 taattcaaca aattaatcgc aagcatagaa ccgcccagaa aagttacaag cacatatcaa
     1501 gcttctttca aaaaattgtt tttgctttca aagcagggaa atctgttgat gaagctataa
     1561 gtgaaattgt atctagtcga gacttcgaat acttaacatt tcaaaagccg tctgaccagt
     1621 ctgattcctt ggcaaaagat tttaatacag gtaaaaaaag tgccatttat atgagagacg
     1681 cattagaaaa ggcaccaaaa tgccatatct gtggcggctt aattcataga aattcaatat
     1741 caattgatca tattcaacga aaacaagatg gtggtctggc gaccttagat aacggtcaa
//
LOCUS       DATBTE010000379         6720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1486540, whole genome shotgun sequence.
ACCESSION   DATBTE010000379 DATBTE010000000
VERSION     DATBTE010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486540"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="VIG33_14225"
     CDS             <1..1106
                     /locus_tag="VIG33_14225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HEY8272044.1"
                     /translation="IGYFGWVGLAYSLKFIWAPLIDRFTLFKLGRRRSWMLTTQVLLV
                     ASIAALGIFDPKTSLMSMAALSVLTAFFSATQDIVIDAYRRELLKDEELGFGSSMNMY
                     GYRIAMLFSGGLAIGLVGNASWNISWNELYYLMSALMGVGILATLWAPEPPASQGVPK
                     TLLSAVIDPFREFLRRDGAYVILTFVFLFKLGDALSGAMLNPYYVQMGFSNADIGLIA
                     KTFGLVSSLVGLFIGSTFMYYIGIFRSLWIFGILQALSTGTFALLTFTGPQNWALALT
                     VCFEDITSGMGTAAFIAFISAVTNKRYTATQYAILSSIATLGRNFFSGFSGDMVKGLG
                     WANFYYACALIAIPGILMLFFMRKYQQPTKSKA"
     gene            complement(1103..2182)
                     /locus_tag="VIG33_14230"
     CDS             complement(1103..2182)
                     /locus_tag="VIG33_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272045.1"
                     /translation="MPFYAQTFAVASAFFLVGCVIGCGSSTVRLPASFKPKYDSQIVT
                     SLKSAALNGVLFQVSEGVFRDVPIQQVDNCRKTEAPNWTANFMNLLDLLNKNPQYYGK
                     FHVVDFKRGDKAKAEISRDIDGLSYLNITYAKRETRDKVTPATRLPCSEGISEFLGKD
                     LVTTFIDWPNSEEINSVLKQASAKAKIERFKFNTEFLVFLAEHQTILKIAPEVAFERS
                     YEGEYFLGSWLEKMAQELRQKDTNIDFINYWLKEIGTHSNQATTIEFFGLHPESSLSY
                     GVQVDTVGRFSRKLNNYQEPTYLFMSYRQHNGAYVYNSLADLNKCLQGLMVIYSNALT
                     MGTSLESDNDSFLAPGYSCKPETKD"
     gene            2409..2499
                     /locus_tag="VIG33_14235"
     tRNA            2409..2499
                     /locus_tag="VIG33_14235"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2443..2445,aa:Ser,seq:gga)
     gene            2760..2948
                     /locus_tag="VIG33_14240"
     CDS             2760..2948
                     /locus_tag="VIG33_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272046.1"
                     /translation="MLRFICWALALGLAVGFGPSLADLTINMAKAAIRASEHDQMSYA
                     RFTKTLLNAKPRAIPKKP"
     gene            2992..3315
                     /locus_tag="VIG33_14245"
     CDS             2992..3315
                     /locus_tag="VIG33_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272047.1"
                     /translation="MSAVTSIPQRKLSTQMEAVLLIFKADPYLMESLKSFINLDTETI
                     HWPQIFKISFGSGHRAAITMAYGVWTDELRPGSNPFDAALSLPPKLQIAVLQALALRW
                     GLKRL"
     gene            3390..3785
                     /locus_tag="VIG33_14250"
     CDS             3390..3785
                     /locus_tag="VIG33_14250"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY8272048.1"
                     /translation="MSSCFYGFPIVAYISLALENEKSSAFKSVQEYCRNTSVETDLFA
                     EEMLPEENDRPLWQKILDLVERNEISMIVVPSLKHISGKDSKSLSSFLTLLKIKSIRL
                     KSIAEGIDSQRDSRLKILSHLNKENAPRQ"
     gene            3824..4144
                     /locus_tag="VIG33_14255"
     CDS             3824..4144
                     /locus_tag="VIG33_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272049.1"
                     /translation="MSENAAKWERKLSREILSVLLIVSADFYLQQSTRQYISVEDQTV
                     NWEALFRLPLNESHKAALTWAYCIWKNEIPRGDNPFVMASSMEPEMKKVILEALKFRW
                     STVS"
     gene            4217..4927
                     /locus_tag="VIG33_14260"
     CDS             4217..4927
                     /locus_tag="VIG33_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEY8272050.1"
                     /translation="MITDKLVFLYLRVSTTEQNVESQKFALLDWCAKQGIMNYEMFVD
                     HGISGAKESRPALNQMMDRVDKGECSQVIVFSFSRFARSVSHLLKALQKFKDKGVRFN
                     SVTEQLDTNSPMGLALFTILGALAQMEREIIRNRVIAGLANARAKGKIIGRVRKRNSA
                     LIESLLEAGLSFRECARVAGCSHGSISAQKKEWVAKKKLAREKEESQKAQNTSIQKEE
                     PMEPSPLPPQTEVQAPTS"
     gene            complement(5216..5641)
                     /locus_tag="VIG33_14265"
     CDS             complement(5216..5641)
                     /locus_tag="VIG33_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272051.1"
                     /translation="MKSILMSIIVMMSITSTSAMANDLKVIESTETEILHLLTNVQTI
                     TLGNYSARIITLSSFAEPGNTYYLLLSQDAGFDPEETNTSLLDLSEGLGLKSDSHIKS
                     VKKVSSNQLEVSIEKNNKISTVKINVNGLKASISNHSHR"
     gene            complement(5913..>6720)
                     /locus_tag="VIG33_14270"
     CDS             complement(5913..>6720)
                     /locus_tag="VIG33_14270"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8272052.1"
                     /translation="KSKNDSEQNALKRFLKHFRSDHPKLKTILVTDALHANQPLLKEL
                     EKHQMSFILGVKPGSHEKLFAAIDKWDKLQRTNHLTREEEIGDKIKKKRVREYRYVNG
                     MLLNHQDVNTAINYLEFWETIQWVDRKGDLKETKVHFSWVTDFSLYESNVEEMARLGR
                     ARWKIENETFNTLKNQGYEFEHNFGHGYKNLSSNFMLLMMLAFLVDQLQEVGCKLFGK
                     ALQTEFGKRSRLWEKFTNLYEFLPAQITDWNMFLSLIAEPEEWTLTRNTS"
BASE COUNT         1806 a         1473 c         1407 g         2034 t
ORIGIN      
        1 ccattggtta ttttggctgg gtgggactgg cttactcttt gaaattcatt tgggccccct
       61 tgatagaccg tttcactctc tttaagttgg gtcggcgtcg cagttggatg cttaccaccc
      121 aggttttact cgtggcatcc attgccgctc taggaatatt tgatccaaaa acatcactga
      181 tgagcatggc agctctgtct gtgctaacgg ctttctttag tgccactcag gatatcgtga
      241 tcgatgctta tcgccgtgag ttattaaaag acgaagaatt gggttttggt tcttcgatga
      301 atatgtatgg ctatcgaatc gcgatgctct tctcaggagg cttggcgatt ggactcgtag
      361 gcaacgcgag ctggaacatc agttggaacg agctctatta tttgatgtcc gccttgatgg
      421 gcgtgggtat cttagcaaca ttgtgggcac cggagcctcc agcttcccaa ggcgtgccaa
      481 aaactttact gtccgccgtg atagatcctt tccgtgagtt cttacgtcgt gacggtgctt
      541 atgttatttt gacttttgtt ttccttttta aactcggtga tgccctctct ggagcgatgc
      601 taaatcccta ttatgtacag atgggtttta gcaatgctga cattggtctc attgccaaga
      661 ctttcggatt ggtttcttct ctggtcgggc tcttcatcgg aagcaccttc atgtactata
      721 ttggaatttt ccgttctctt tggatctttg gaattcttca ggctctttca acggggactt
      781 ttgctttatt gaccttcacc ggtccccaga actgggccct cgctttgact gtctgctttg
      841 aggacatcac ttcaggaatg ggaacggcag cgtttattgc ctttatttct gctgtgacca
      901 acaagcgcta tacggcaact cagtacgcga ttctctccag cattgccact ttaggaagaa
      961 actttttctc gggattttct ggagacatgg tcaagggcct cggctgggct aatttttatt
     1021 atgcctgcgc cctcattgcc attccaggaa ttttaatgct gttctttatg cgaaaatatc
     1081 aacagccaac aaagtccaaa gcttagtctt tggtttcggg tttgcaagaa taacccggag
     1141 ctaaaaagct atcattgtca gactctaagc tggtccccat ggtgagcgca ttgctataga
     1201 tcaccatcag gccttgcaga catttattaa ggtcagcaag actattatag acatacgcgc
     1261 cgttgtgctg acgataggac atgaacaaat aagtcggctc ttgataatta ttcagtttac
     1321 gtgaaaatct tcctacggtg tccacctgca caccataact taagctgctc tcagggtgaa
     1381 gtccaaaaaa ttcgatcgtg gttgcttgat tactatgggt gccaatttct ttcagccaat
     1441 aattaataaa atcaatgtta gtatcttttt ggcgaagttc ctgggccatt ttctcaagcc
     1501 aacttcctaa aaaatactct ccttcataac ttcgctcgaa ggccacttcc ggagcgatct
     1561 ttaagatagt ctgatgttca gctaaaaaaa ctaagaattc ggtattaaat ttaaaacgct
     1621 caattttagc tttagccgaa gcttgcttta acacagagtt aatttcttcg ctattaggcc
     1681 aatcaataaa agtggtcaca agatctttac ctaagaactc cgaaatccct tcagagcaag
     1741 gcaaccgtgt cgcgggagtg accttatcgc gagtttcacg tttggcatag gtgatgttca
     1801 aatagctcag accatcaatg tctcttgaaa tttcagcttt ggctttatct ccccgtttaa
     1861 aatcgacaac gtgaaatttc ccatagtatt gaggattttt attaagaagg tccaaaagat
     1921 tcataaaatt cgcagtccaa ttgggagcct ctgtcttacg gcagttatcc acttgttgga
     1981 tcggaacatc tcgaaaaacg ccttcgctca cttggaacaa aactccgttt aacgccgcag
     2041 atttcagaga ggtcacaatt tgagaatcat acttaggttt aaaggaggcc ggaagacgta
     2101 ccgtcgatga tccacaacca attacgcaac caactaaaaa gaatgccgag gcaacggcaa
     2161 aagtttgagc ataaaagggc atgagacctc cgtttcctag tatatcgaat ttgagatgcc
     2221 gctttcatct ttcacgactt ttccgtagat tagtccatag acctgggcct tggctacgtc
     2281 ctaagcctca actcggccca taaaaaatca aattaggctc aattttaggg caattcatag
     2341 aattctgatc tttaccccgt ctggacctcg tgctaacaat aacagcttct tatgtatccc
     2401 aagtctgcgg agggttggcc gagtggctga aggcgctcgc ttggaaagcg agtttacgcc
     2461 aaaagcgtaa cgcgagttcg aatctcgcat cctccgccat tcttcgtgcc tcattaagag
     2521 tgaagtttca aaactcaaat caaaataaac agttacacaa caaataacgt gagcaggcac
     2581 tctgctcggc atttcagcat atttcacggt atttcaaaat atggtagggt tttggtaggc
     2641 tcctggtagg gttccaaaat cgctacaatc ttagcagtcg aaaacagcaa tcactcttct
     2701 tctttctgat tttcatttta tcacttggga ttttttaaat cccggaagag gagagattta
     2761 tgctgcgttt tatttgctgg gcactagccc tgggtttggc cgtgggcttt gggccttcgc
     2821 tcgcggatct tactattaat atggcaaaag ctgccatccg tgcctctgag cacgatcaga
     2881 tgagttatgc gagattcacg aaaactttgt tgaatgcaaa gccacgggcc attccgaaaa
     2941 agccataggg tcccagaata tttaccttaa cttatttttt aggagtgttt tatgtcagca
     3001 gttacgtcaa taccgcagag aaaattatcc acccagatgg aggcggtgct tttaattttt
     3061 aaagccgatc cctatcttat ggagagtttg aaatctttta ttaacttgga tactgaaacc
     3121 attcattggc cacaaatttt taaaatatca tttggctccg gccaccgggc tgccatcacc
     3181 atggcctatg gagtttggac tgatgagctt cgtccaggga gtaatccctt tgatgcggcc
     3241 ctcagtctgc cccccaagct acaaatagcg gtcttgcagg cgctggcttt gcgttggggc
     3301 cttaaaagac tttaaacttt ttgttttata ttttttttct aatcactaag cccgagatcc
     3361 cctcgggctt ttttattttg gaggtccgct tgtctagttg tttttatggt ttccccattg
     3421 tcgcctatat ttctttggct ttagaaaatg agaaatcctc agcctttaag tcggttcagg
     3481 agtattgccg taacacgtcg gttgaaaccg atcttttcgc cgaagagatg ctgccggaag
     3541 aaaatgatcg tcccctttgg caaaagattt tggacctcgt tgaacgaaat gagatctcca
     3601 tgattgtggt cccaagtctt aaacacattt ccggaaagga ttcaaaatcc ctttcctcat
     3661 ttttaactct tttaaagatc aagagcattc gcctaaaaag catcgctgaa ggaattgaca
     3721 gccaacggga ttcacgtctg aaaattttat cgcaccttaa taaggagaac gccccccgcc
     3781 aatgagccaa aaattaactt tgaaaccatt tttggaggac ttaatgtcag aaaacgcggc
     3841 taaatgggaa aggaaactct ccagggagat tctttcagtc cttttgattg ttagcgcaga
     3901 tttctatttg caacaaagca ctaggcagta catcagcgtt gaggatcaaa ccgttaactg
     3961 ggaggccctt ttcaggctcc ccttgaatga atcccacaaa gcggccctaa cttgggcgta
     4021 ctgcatttgg aaaaacgaaa ttcccagagg agacaatccc tttgtgatgg cctcatccat
     4081 ggaacctgaa atgaaaaagg tgatccttga ggctctgaaa tttcgctgga gtacagtgtc
     4141 ctagatttaa ttttcaaatt tcattttttt tcaaggctca aaatattcga gccttttttt
     4201 attttccgga gggaaaatga ttacagataa gcttgtgttt ttgtatttaa gagtttccac
     4261 gactgagcag aatgtcgaga gtcaaaaatt tgccttactt gactggtgcg caaagcaagg
     4321 tatcatgaat tacgaaatgt tcgtggatca tgggatttca ggagctaagg aaagtcggcc
     4381 tgctctgaac caaatgatgg atagagttga caagggcgaa tgctctcagg taatagtttt
     4441 ttccttctca aggtttgcaa gaagtgtcag tcatctcctt aaagctctgc aaaaatttaa
     4501 ggataaagga gtgagattca actccgtaac tgaacagctt gatacaaatt ccccaatggg
     4561 attagctttg ttcacgatcc tcggcgcgct ggcacaaatg gaacgcgaaa ttatcagaaa
     4621 tcgtgtcatc gctggcctcg ctaatgctcg cgccaaagga aaaattattg gccgagttcg
     4681 caaacgaaat tcagcactca tagaaagttt gcttgaagcc ggactttctt ttagagagtg
     4741 cgcacgagtg gcgggctgtt cgcatggtag tataagcgcc cagaaaaaag agtgggttgc
     4801 gaagaaaaaa ctagctcgtg aaaaagaaga atctcaaaag gcccaaaaca catcaattca
     4861 aaaagaggaa cctatggaac catcgccatt gccaccgcaa actgaagttc aagcacccac
     4921 ctcgtaaaca agttcaccgt aatatcattt ctgccgtcga ttctcaattt agagagtcga
     4981 cggctttttt tttgccttgt gtgaatagga acgcgggcct ttcgttccag ttcacagaat
     5041 ttttgaaatt ttcaagcatg cctgcgcttt gttgacagag atgttaccga ggtcgaattt
     5101 cggccccagt ctatattact ccgtcgagaa gtcaggcgct aaaaccaagg gaattcggcc
     5161 cgcaaatctg taaagatttg tccccctacg gtacgtgaga tctcgagtcg gaaagctagc
     5221 gatgactgtg attactaatc gacgccttta gtccattcac atttatcttt actgtcgaaa
     5281 tcttattatt tttctcaata gagacctcaa gttgatttga actcactttt ttcacagatt
     5341 taatatgaga gtcagatttg agacccaggc cttcgctcaa atccaacaga gaggtattag
     5401 tttcttcggg atcaaaaccc gcatcttgcg aaaggagtaa atagtatgta ttgccaggtt
     5461 cagcaaagga actcagcgtg attatgcgtg cagagtaatt tcccaaggta atggtttgga
     5521 catttgttag taaatgaaga atctcggttt cggtgctctc gataacctta aggtcattcg
     5581 ccatcgccga tgtggaagtt atagacatca taacgattat tgacataaga atacttttca
     5641 tttgggtttc ctttcaagtt gttggttaca ttatcatact ctgcatgtga tgtgccaaaa
     5701 gatataggga ctgatcgagc caggaactcc cggtactcca ttaggttggc ttttgttttg
     5761 aggtcgggac gctttttgct cctatttttt ctaatttctc tgggaggaaa atgattttga
     5821 caaaaagata tacattgcca gaagggattt ctgacaaacc tcagtacttc cataaatttt
     5881 tcttatactt taagggagag attttctact ttttaagaag tgtttcgcgt gagcgtccac
     5941 tcttcaggct ctgcgatgag gcttaaaaac atattccaat cggtgatttg agcgggcaaa
     6001 aactcataca agttagtgaa cttctcccaa agcctgcttc gttttccaaa ctctgtctgc
     6061 aaagcttttc caaataattt gcagccaacc tcctgaagtt gatcaactaa aaaggccaac
     6121 atcattaaca acataaagtt tgaagataaa tttttatagc cgtgaccgaa attatgctca
     6181 aactcataac cctgattctt caatgtattg aatgtttcat tctctatctt ccaacgggct
     6241 ctcccaagac gagccatctc ttcaacgttg gactcataaa gagaaaaatc cgtgacccaa
     6301 ctaaaatgca cttttgtttc cttaagatcc cctttgcgat ccacccactg aatggtttcc
     6361 caaaattcta ggtagtttat tgcagtattt acatcttgat gatttaacag catgccattg
     6421 acatagcgat actcccgcac tcgtttcttt tttattttat cgccaatctc ttcttctctt
     6481 gtcagatgat tcgttctttg aagcttatcc cacttatcaa tagcggcaaa gagcttctca
     6541 tgactaccgg gcttaactcc taaaataaaa ctcatctgat gcttctcaag ctcctttaat
     6601 aacggctgat tggcatgcaa agcgtctgtt accaatatcg tcttaagttt cggatgatcg
     6661 gatctaaaat gctttaaaaa tctttttaaa gcattctgtt cggagtcgtt cttggatttt
//
LOCUS       DATBTE010000380         3623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_597552, whole genome shotgun sequence.
ACCESSION   DATBTE010000380 DATBTE010000000
VERSION     DATBTE010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597552"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2886
                     /locus_tag="VIG33_14275"
     CDS             <1..2886
                     /locus_tag="VIG33_14275"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8272053.1"
                     /translation="QSGIMIIYPWYQELSQLQDILWGLDDQLFLDNGERPGFEVIGEF
                     GTKAILKYLRTRRIPVNLAGNSITLGTHQSQTEVHVDERGSFCVQQEARIAGQKNLRR
                     MGWTSRSTLYLQILSQGLPFLLKAEAKDIASRSSMKREWDLKLLKHLGILQYLFLEIL
                     SFHFEGRLFDGTEVQENNLFLQIHQNIQRLLLAGTEAVFIRDLPLTTLCSKSVLACFK
                     DFVHVTLKTLSGTECFYTEHGEVILEGVVEREFRLIYELLKKMAFTSKGEAFKKARTP
                     FLAKVSAGKFSDVEELAMALFHFPTGDLKNASSLQSTLESLQLLVPHDFKIYFKGQAI
                     QELQDDEFRVDFILQSEGESEGERKFFNWFELNPKFFLRGEEVDPQQLVNLGGGGLME
                     YKGKFFLIPQRQMPSLRRLEHFWLRLQKGKVDVQKKKNGDAIYKLPRSQTLELLALRA
                     SGVEIRGDEEWIKLCYFYDRLGVIDRERKLPQSSRYILKSYQAAGVLWLQDLYRLRLG
                     ALLADDMGLGKTLQTLCFLDDLRQKKEMGQVLIVMPSSLIFNWRNEIEKFTPELAIKV
                     FAKNDMDALGRRLEAKEEMIVLITYGLLLEHEEFINQYKWKVLIFDEAQNLKNISTKR
                     TSAARSLSAQFRICLTGTPMENHYGEFYSLVDILVPGSLGNIENFRRQFVNSEMVTRE
                     EMEDLKLKIKPLLLRRTKKEILDQLPEKQETKVSIAFEDRQMEIYRDIALSYNQKVQE
                     ALKSGEGSQGAASVQLQMLTALLRLRQACSDPSGLPNVRYEKVPPKLETLLDSLREII
                     ESGESVLVFTQFLQTLEHAAKRLKMANIPVFVLHGGVSVPQRQKILAEFEKVAGGAVL
                     LMTLKTGGVGLNLTKASYVFHLEPWWNPSVENQATDRAHRMGQCKAVQVFRYIMHESL
                     EEKIELLKSRKERKFQALFSDTEKDLEVGMGSNALSKQDFDFLLGLR"
     gene            3116..>3623
                     /locus_tag="VIG33_14280"
     CDS             3116..>3623
                     /locus_tag="VIG33_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272054.1"
                     /translation="MKRNSVHAEIVSSLAKLAMISSLASACGGKSFDTPEDSQLRPPA
                     TSDPLTASEQRQWEAKTTLGPVRELPPRKSILETKVERETFLQDVTEQTPTHSLEQLT
                     TALEDQFEKNQKELIKDKDKDKDPNPPVVPPNPTEPVKKSDVKPELQGSAASRDWCKE
                     IDVNSEKEE"
BASE COUNT         1026 a          712 c          904 g          981 t
ORIGIN      
        1 caatcgggaa tcatgataat ctatccttgg tatcaagaat tgagtcagtt gcaggatatt
       61 ttatgggggc tggatgatca gctcttcctg gacaatgggg aaagacctgg ctttgaagtg
      121 atcggggagt ttgggactaa agcgatttta aaatatttac ggactcgaag gatccccgtc
      181 aacctcgcgg gaaattccat cactttgggg actcatcaaa gtcagaccga ggttcatgtg
      241 gatgagagag gaagcttctg tgtgcagcag gaagcccgaa tagcgggtca aaaaaatctg
      301 aggcgaatgg ggtggacctc tcggtccaca ctctatttgc aaattctttc gcaaggtctg
      361 ccatttttat taaaggccga ggcaaaagat attgcgagtc gttcttccat gaaaagagaa
      421 tgggatctga aacttttaaa gcatttagga attctgcaat atcttttcct ggaaattctt
      481 tcttttcatt ttgaaggaag gctatttgac ggaacagaag tccaagaaaa taatctattt
      541 ctgcaaatac atcagaatat tcaaaggctt ttactggctg gcaccgaggc tgttttcatt
      601 cgagatctac ctttgacgac tctttgttca aaatcagttt tggcgtgttt taaagatttt
      661 gtgcatgtca ctttgaaaac cttgagtggc acagagtgtt tttatactga gcatggcgaa
      721 gtcatcttgg agggagtcgt cgaacgcgag ttccggctca tttatgaatt gcttaagaaa
      781 atggccttca cttctaaagg cgaggctttt aaaaaagcaa gaaccccctt tttagccaag
      841 gtgagtgcag gaaagttttc tgatgtggaa gagttggcga tggcgctttt tcattttccg
      901 acaggagacc ttaaaaatgc ttcgagcctg cagagcaccc tggaatctct gcaactattg
      961 gttccccatg attttaaaat ttattttaaa gggcaggcca ttcaggaatt gcaagacgac
     1021 gagtttcgcg tggactttat tttacaatca gaaggggaga gcgaggggga gcgaaagttt
     1081 tttaattggt tcgaattgaa tccgaagttc ttcttgcgcg gcgaagaagt ggatccgcag
     1141 caattggtaa acctgggagg cggtggcttg atggagtata aaggaaagtt tttccttatt
     1201 cctcaaaggc aaatgccgtc tctgcgaagg cttgagcatt tctggctcag attgcaaaaa
     1261 ggcaaagtgg atgtgcaaaa gaaaaaaaac ggagatgcta tttacaaatt gccacgcagt
     1321 cagactttgg aacttttggc cttaagggcc tccggggttg aaatccgtgg ggatgaagag
     1381 tggataaaac tctgttattt ctatgatcgt cttggtgtca ttgatcgtga gcgtaaactc
     1441 ccacagtcat caagatatat tctgaaatct tatcaggcgg cgggggtgct gtggctgcaa
     1501 gatctttaca ggttgcggtt aggtgctctg ctcgcggatg atatggggct tggtaaaact
     1561 cttcagacac tctgctttct cgatgatctt cgtcagaaga aggagatggg gcaggtcctg
     1621 attgtcatgc cttcttcttt gattttcaat tggcgaaacg agatcgaaaa gttcacgccc
     1681 gagctggcca ttaaggtgtt tgccaaaaat gatatggatg ccctaggacg aagactcgag
     1741 gcaaaagaag aaatgatcgt gcttatcact tatggacttt tgttggaaca tgaggagttt
     1801 ataaatcagt acaaatggaa agtgctgata tttgatgaag cgcagaacct taagaatatt
     1861 agcaccaaga gaacgagcgc ggcgcgttcg ctctctgcgc aatttagaat ttgcctgacc
     1921 ggaaccccca tggagaatca ttatggagag ttttattctc tggtagatat tcttgtgcct
     1981 ggaagccttg gcaacattga aaacttccgc cgccaatttg taaattctga gatggtcacg
     2041 cgggaagaga tggaagatct gaaactgaag atcaaacctt tacttctccg tcggaccaaa
     2101 aaggaaattt tggaccagct gcctgagaag caagaaacca aagtgagcat cgcctttgag
     2161 gatcggcaaa tggaaattta tagagacatt gctctttcct acaatcaaaa agtacaagaa
     2221 gccctaaaga gcggtgaggg atctcaaggc gcagcgagtg tccaattgca gatgttgaca
     2281 gccttattaa gacttcgtca ggcgtgttct gatccttccg gtttaccgaa tgtgcgttac
     2341 gaaaaagtac cacctaagct tgagactctt ttagattccc ttcgtgaaat tattgaatct
     2401 ggtgaaagcg ttctggtctt cacgcagttt ctgcaaaccc tggagcatgc ggcaaagaga
     2461 ttgaaaatgg cgaatattcc cgtttttgtc ctccatggtg gagtgtcagt gccgcagcga
     2521 caaaaaattc ttgccgaatt cgaaaaagtc gccggtggag ccgttttgtt gatgactttg
     2581 aaaaccgggg gagtcggctt aaacttaact aaagcaagtt atgtttttca tctggagccg
     2641 tggtggaatc cttcggtgga aaaccaagcg acagatagag ctcaccgcat ggggcagtgc
     2701 aaagccgttc aggtttttcg atatatcatg catgagtctt tagaagaaaa aatcgaactg
     2761 ctaaaaagcc gcaaagaacg aaagtttcaa gctcttttct cagatacaga aaaggatctt
     2821 gaggtgggca tgggcagcaa tgctttgagt aaacaggatt ttgattttct tttagggctt
     2881 cgctagccta actttgtctc taacagagga agttttttta gggtggtttc cgtttgtaga
     2941 aaacaggtat ttttttccac aaaaaagtct gaaagattca agctccttag cctctttccc
     3001 caagaaagct gtgtgatgat ttgacagagt actgtggatt tcattgcggc ataggagttg
     3061 caaacttgtt atcggcaatg aggccctttg acgaaaaagc ggcggaaaga aaactatgaa
     3121 acgtaattct gttcatgctg aaattgtaag ttctctggca aaactcgcca tgatttccag
     3181 tttagcgtcg gcctgtggtg gaaagtcttt tgatactcct gaggattcgc aacttcggcc
     3241 ccccgctact tcagatcccc tcactgccag tgagcaacgt caatgggaag ctaaaaccac
     3301 tctggggccg gtccgtgaac ttcccccaag aaaatcaatt ctagagacta aagtggagag
     3361 agaaaccttc cttcaggatg ttacagagca aacgcccacc cattcactag agcagcttac
     3421 cactgccctg gaagatcagt ttgagaagaa tcaaaaagag cttataaaag ataaggataa
     3481 agataaggac ccaaaccctc cggttgtgcc ccccaaccct acggagcctg tgaaaaagtc
     3541 tgatgtaaaa cctgaactgc aggggtccgc tgcctctcgg gattggtgta aagagattga
     3601 tgtgaattcg gaaaaagaag agg
//
LOCUS       DATBTE010000381         5477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1691425, whole genome shotgun sequence.
ACCESSION   DATBTE010000381 DATBTE010000000
VERSION     DATBTE010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691425"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..743)
                     /gene="thyX"
                     /locus_tag="VIG33_14285"
     CDS             complement(111..743)
                     /gene="thyX"
                     /locus_tag="VIG33_14285"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEY8272055.1"
                     /translation="MTYENVAFVSLTPNAEETMAYCARVSNVDNQNNPNVAGLLSYCI
                     RHGHWSVFEQANMVLEINTSRAIAPQILRHRSFSFQEFSQRYAEASDFMTFSARRQDQ
                     KNRQNSIDDLDSETQNWFTTSQSEIWQIAYAKYLEALKKGIAKECARAFLPLNTSTKL
                     YMNGTIRSWIHYIQLRAQSNTQLEHRNIALLCQRIFKDHLPQVSEALEWK"
     gene            841..1179
                     /locus_tag="VIG33_14290"
     CDS             841..1179
                     /locus_tag="VIG33_14290"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8272056.1"
                     /translation="MENIPVHLLNAFVVFNESKSIGEAAVRLQITQPALSKQLKQLEE
                     RLPNAVFTFSGRKKVLTSLVENFINASEKELATYRNWYGRLGRFIQIQARPRLKFQQG
                     EGFWIECHPK"
     gene            1164..1733
                     /locus_tag="VIG33_14295"
     CDS             1164..1733
                     /locus_tag="VIG33_14295"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY8272057.1"
                     /translation="MSSKIKFNGTIFFLERSNEEIIESLLDLDLEVGVIHRIPPNTQE
                     LIAKPLFKEEFQLVIPKSFLSSRHVFGDALFYQLKRLPCLGYRPHDEVLRTVCSFTST
                     DMQTLKMMRATENYLSIVEMVEAKMGWAVLPTYLNVSEAKNWIVTIPARAFPARQFYL
                     VYRQEFSSVPWFKDLISEIRSCFTPHWCG"
     gene            complement(1730..2548)
                     /locus_tag="VIG33_14300"
     CDS             complement(1730..2548)
                     /locus_tag="VIG33_14300"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HEY8272058.1"
                     /translation="MKKLRIALTFSTVATILACESHMSSGGNSMLISSSLDSPFLSSY
                     PSGNNNLRISLTDAPSKDLKNVFINVDHAELFVRKGSGEGRLIVAQDLGLIDLMTLRN
                     GVLLPLEDLSLPAGLEITAIRLVLKADNNHSIKADDSRCEMQTPSGQQSGIKIHLSQP
                     FVLEQGQAYSMVMDFDAAKSVVVKGNGGCLLKPVLKLLSVTKSSGDAGEDASSTDASG
                     ETPVTDGADTNISTETTSDSTSDSSGSTGFEVPPADGSTTEPPVMTVQDTFNLA"
     gene            3019..3792
                     /locus_tag="VIG33_14305"
     CDS             3019..3792
                     /locus_tag="VIG33_14305"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY8272059.1"
                     /translation="MSPALKLEGKLFALAFFFLAFCFGMTVYAVYKYGLDVPTCLTSA
                     EPFTKGQVIVRAPDHYEIHYVAKMWEFDPAVVNVRTGSTLDIYLNSIDVTHGFQIPGT
                     NANLMAVPGAVNYVKLFLKNPGQYNVVCHEYCGLSHHNMIGVINVSDNPLPNNQGVPE
                     ALTSQAEAEGLKLLQVKACVGCHSTDGSPRVGPTFKGIFGRTVMLDDGVTIKVDEAYL
                     IESIRQPQVKVVQGYQPLMPLIPLSDQEIQVIIQYLRWL"
     gene            3802..>5477
                     /locus_tag="VIG33_14310"
     CDS             3802..>5477
                     /locus_tag="VIG33_14310"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY8272060.1"
                     /translation="MVNIQPALRKIIIFELVFPIVLLIVGIYHGLLQFLYRAGWLRQV
                     SFGGLEYYQGLTLHGVINAIVFTTFFAVAFGNALIPYFLGKHPSVKVAVISGVTMAIG
                     TVMAAIPIFTGSASVLYTFYPPLKAHPSFYIGVTLLVVGSWLAFWGWIPVYRSWRKEN
                     PGKKIPLAILGIFTTFLVWQIATAPVAYEVLVLLLPWSLGWTDTVNVVLARTLFWFFG
                     HPLVYFWILPAYIMYYTMLPKLVGGKLFSDFAGRLAFLWLLVFSSPLGLHHQFTEPGL
                     DSNWKLAHAFLTFLVAVPSIMTAFVVAASMEYGARARGGKGLFGWWSKLPWFEQDAWL
                     FGYFICGLILFIFGGITGIVNASYSMNRVVHNTAWLPAHFHMTVAGPAFLAFIGMSLH
                     MISQLTGKAIRYPSMNLWFPYIWSIGIFVSSTGAFIGGLQGMPRRTNMGLTYLNPESV
                     YFRGDWIFPSNIAVIGGIIMTIAMVMFFTVVFATLFSKKTMQEGIEFPVAEAYHDEDY
                     AVVRNMKPWIAAAVAAVAIAYTPPLYEIYKNKVKGAAAFLPSSPVPASGTG"
BASE COUNT         1396 a         1111 c         1255 g         1715 t
ORIGIN      
        1 cactcactgc tgagcgaaga ccttcagctt ttcaaaaaaa acataatttg cttaaatttt
       61 aacccacgaa ttattcaaac gaaccgattt tctgtggaaa gaattaaaga tcatttccat
      121 tcgagggctt ctgatacttg aggtaaatga tctttaaata tccgctgaca caataatgca
      181 atatttcggt gctcaagctg cgtgtttgat tgagcgcgaa gttgaatgta atgaatccag
      241 cttctaatgg tcccgttcat atacaatttt gttgaggtat tgagtgggag gaacgctctt
      301 gcacattctt ttgcaattcc tttttttaag gcttccaaat atttcgcgta agcaatctgc
      361 caaatttcag attgtgaagt ggtgaaccaa ttctgcgtct cggaatccaa atcatctata
      421 gaattctgac ggtttttctg atcctgtcgg cgcgcgctaa aagtcatgaa atcgctggct
      481 tcggcatatc gttgagaaaa ttcttgaaat gaaaaagaac gatgtcgtaa aatctggggc
      541 gcaatggctc ttgaagtatt aatttccaga accatattcg cttgttcaaa gaccgaccaa
      601 tggccatgtc taatgcagta gctgagaagg ccagccacat ttggattgtt ctggttatct
      661 acattgctaa ctcgcgcgca gtaagccatg gtttcttcag cgttaggagt aagggaaacg
      721 aatgcgacat tttcgtaagt catgatggac ctcctggtct ttgtgtagaa tcccagaaaa
      781 tctttttttg cataaggaat atgatttcat attttatatt ctttaagtta tatgttgaaa
      841 atggaaaata tacctgttca tcttcttaat gcctttgtcg tattcaatga atcaaagagt
      901 atcggcgaag ctgccgtacg ccttcaaatc acgcagcctg cgctgtctaa gcaactcaaa
      961 caattggaag agaggctgcc gaatgcggtc tttactttta gtggcagaaa gaaagtgctc
     1021 acctcattgg tcgagaactt catcaatgcc tcagaaaaag aattggcaac atacaggaat
     1081 tggtacggca gacttggacg cttcattcag atccaagcca ggccaagatt aaaatttcag
     1141 caaggcgagg ggttttggat cgaatgtcat ccaaaataaa atttaacggt acgatatttt
     1201 ttctggagcg atcgaacgaa gagattatcg aatctcttct ggatcttgat ttagaggtgg
     1261 gtgttattca taggattcca ccgaacacac aggaactgat tgcgaagcct ctctttaaag
     1321 aagaattcca attggtaatt cctaagagtt ttctttcaag tcgacatgta tttggggatg
     1381 cgctttttta tcaacttaag cggctgccat gtttaggata tcggccgcat gatgaggtgt
     1441 taaggacagt ctgttctttt acttccaccg atatgcagac gctaaaaatg atgagggcca
     1501 ccgaaaatta cttaagcatc gttgaaatgg tggaggcaaa aatggggtgg gctgtactgc
     1561 ccacttatct gaatgtttct gaagctaaga attggattgt cacaattcct gcgagggcat
     1621 ttcctgctcg gcagttttac cttgtctacc gacaggagtt ttcctctgtc ccttggttta
     1681 aggatttgat ttcagaaatc cgatcttgct ttaccccgca ttggtgtggt taagctaagt
     1741 tgaatgtatc ttgcacagtc atgacaggag gctcagttgt tgaaccatca gccggtggta
     1801 cttcaaaacc cgtgcttccg ctactgtcag aggtggaatc gctggtagtt tccgtgctga
     1861 tgttggtgtc tgctccatca gtgactggag tctcgcctga agcatcagtc gaagaggcat
     1921 cctccccggc atcccctgag cttttagtaa cgcttagaag tttcaacact ggctttagta
     1981 agcatccgcc attccctttc accactactg attttgcagc gtcgaaatcc atcaccattg
     2041 aataagcctg tccttgctca agaacgaaag gttggctcaa atgaattttt attccggatt
     2101 gttgaccact tggtgtttgc atctcacagc gagaatcatc tgctttaata gagtgattat
     2161 tgtctgcttt taaaacgagt cgaatggctg tgatttcaag acctgcgggc aaactcaaat
     2221 cttcaagggg cagcaaaact ccatttctca gcgtcatgag atctattaat cccaaatcct
     2281 gagcgacaat aaggcgccct tcaccagatc cttttctgac aaagagttcg gcatggtcca
     2341 catttatgaa gacgtttttt aaatctttgg atggggcgtc ggtaagactg atccgtaggt
     2401 tattattccc ggaaggataa ctgcttaaaa atggcgaatc gagactgctg gatataagca
     2461 tggaatttcc accactgctc atatgacttt cacatgcgag gattgttgcc acagttgaga
     2521 acgttaatgc tatccttaat tttttcatac cgacctcctt ggcccgtgag aaaagaatat
     2581 caacattgag aaaagtagtc gaatttttaa gttcgaacgg agcattcaga gaaggagcca
     2641 aagagtgttt aactttttca tgggactttt taataaagtg tagatttttt ttacgttcat
     2701 cggagttcct ctttttattc tcattcagag aaagggtttt agattttgct caatccacgt
     2761 ctgctgtttt ttggcaaaaa ataatcaaat tttttcattc ttcctagaag ttttaatcac
     2821 aatttgagtt tggaaagcac tctcattgac tttgtttgaa gcaagattga ttcctgcatt
     2881 cgtctaagga ggaagtaatc catggaaatt gtcgactttg ttcctagagg ggctgtggct
     2941 tttttcataa gcctaattgt tttattcacc atgatctgga catttgtata ctggatcatg
     3001 ttgcaaaggg gatagctgat gtctccggcc cttaaactcg aaggaaaact ttttgcatta
     3061 gccttttttt tcttggcatt ttgttttgga atgactgtct atgccgttta taagtatggg
     3121 cttgatgtgc cgacttgttt aacttccgct gaacccttta ctaaaggaca agtcattgtg
     3181 cgggcccctg atcattatga aattcactat gtagctaaga tgtgggaatt tgatccagca
     3241 gtggtgaatg tgcgaacagg atctacctta gacatttatc tcaatagcat agacgtgact
     3301 cacggttttc aaattcctgg aaccaatgct aatttaatgg cagtccccgg cgctgtgaac
     3361 tacgttaaat tgtttctgaa aaaccctggg caatacaacg tggtttgcca cgaatattgt
     3421 ggcctcagcc atcacaatat gattggagtc attaatgtca gcgataatcc gttgccaaat
     3481 aatcaaggtg tgccggaagc cctcacttcg caagctgaag ctgaaggatt aaaattattg
     3541 caggtaaaag cctgcgttgg gtgccacagc actgatggat cgccgcgtgt ggggccgacc
     3601 tttaaaggaa ttttcggacg cacagtaatg ctggacgatg gtgtcacaat taaagttgat
     3661 gaagcttatt tgatcgaatc gattcgccaa ccgcaagtga aagtggttca aggttaccaa
     3721 cccctgatgc cgctcatccc cctgagtgat caagagattc aagtaataat ccaatattta
     3781 agatggctgt aggaggaagt aatggtgaac attcaaccag cgctgagaaa aataattatc
     3841 tttgaactgg tctttccgat cgtacttttg atcgtcggta tttatcatgg cttattgcag
     3901 tttctttatc gcgccggatg gttaaggcaa gtgtcatttg ggggactaga gtattaccag
     3961 gggcttactc tacacggcgt gatcaatgcc attgtcttca ccactttttt tgcggtggct
     4021 ttcggtaatg ctttgatccc ttatttttta ggaaagcatc cctctgttaa ggtcgcggtg
     4081 atttcaggag ttacaatggc catcggcaca gtgatggctg caattccgat ttttacagga
     4141 agtgcttcag tactctatac attttatccg ccattgaaag ctcatccctc attttatatt
     4201 ggagtgactt tacttgtggt tggctcgtgg cttgccttct ggggctggat cccggtgtac
     4261 aggagctggc gcaaagaaaa tccaggaaaa aaaatacctt tagcgattct aggaatattt
     4321 acaacatttc tggtttggca aattgctacg gccccggtgg cttatgaggt tttagtgtta
     4381 cttctgccgt ggtccttagg gtggaccgat acggtcaatg ttgtgcttgc acgaacgctt
     4441 ttctggtttt ttggtcatcc gctggtttat ttttggatct tacccgccta catcatgtat
     4501 tacacaatgt tgcctaagct ggtgggcggg aaattgtttt ctgattttgc cggtcgcttg
     4561 gcttttttat ggctgctggt tttttcttcg cccctgggct tgcatcacca attcacggag
     4621 ccgggactag attcaaattg gaaattggct cacgcatttt taactttttt agtggccgtt
     4681 ccaagtatca tgactgcatt tgttgttgcg gcttctatgg agtatggggc gcgtgcccga
     4741 ggcggcaaag gactttttgg gtggtggagt aaacttccat ggttcgaaca ggacgcctgg
     4801 ctttttggtt actttatttg tggcctgatt ttgtttattt ttggtggcat tacaggaatt
     4861 gtgaatgcat cttatagtat gaatcgcgtt gttcacaata ccgcttggct tccagcgcac
     4921 ttccacatga cggtagccgg tccggccttt ctggccttta ttggaatgag tttgcatatg
     4981 atcagccaat tgactggcaa agcgatccgc tatccctcga tgaatctgtg gtttccttac
     5041 atttggagca ttggaatttt tgtttcctca acgggtgcct ttatcggggg actccaggga
     5101 atgccaaggc gaaccaatat gggacttacc tatcttaatc ctgaatcagt ttattttcgt
     5161 ggtgattgga tctttccctc aaatatcgct gtcatcggcg gaattatcat gactattgcc
     5221 atggtgatgt tttttacggt ggtcttcgca actttatttt ctaaaaagac tatgcaggaa
     5281 gggatagagt ttcccgtggc ggaagcctat cacgatgaag attatgctgt ggttaggaat
     5341 atgaaaccct ggattgctgc cgcagtggca gcggtggcta tcgcttatac gccacctttg
     5401 tatgaaatct ataaaaacaa agtgaagggt gcggccgcat ttctgccaag cagcccagta
     5461 cctgcaagcg ggactgg
//
LOCUS       DATBTE010000382         1628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2375993, whole genome shotgun sequence.
ACCESSION   DATBTE010000382 DATBTE010000000
VERSION     DATBTE010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2375993"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1209
                     /locus_tag="VIG33_14315"
     CDS             103..1209
                     /locus_tag="VIG33_14315"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8272061.1"
                     /translation="MERSLKSQILADLNDKMVLLAGPRQVGKTTLARSLFKNTQYLNW
                     DVDEDRSHILSRSFHPADLWIFDEIHKYKTWRNYLKGLYDKLGNQQKILVTGSAKLDV
                     LRKGGDSLQGRYHFLRLMPLSFKELGMRTTKDTQQLFELSGFPEPFFSQSKSKSNRWS
                     RSYRERIIRQEVATNELIQDLGNMEILLHRLPELVGGTLSINAISEDIQISHKTLSKW
                     ISALERLYAVFRISPFGPPKVKAVKKEQKLFFFDWNAIVDPGGRFENFVAVHLLKWIY
                     FEQDVNGRNLDLRFYKDKAQREVDFVIIENNRPIFFIEAKYGDSEITQGIKYLKQRFP
                     MVRAMQVHLQGKKEYQSDLGIEYVHVNRLLSELI"
BASE COUNT          490 a          324 c          355 g          459 t
ORIGIN      
        1 agctttccgc ttaagtgttt ttcgcaagtc tttttttgga taggtatcca aaaaaagaca
       61 gaacattctt gacacccatc caaaaatgtc taccttgaag ggatggaacg ttccctcaag
      121 agtcaaattc tcgccgacct aaatgataaa atggttttat tggctggccc gcgccaagtg
      181 ggtaaaacca ctttggcacg gtctctgttt aagaacaccc aatatctcaa ttgggatgtg
      241 gatgaggatc gctcgcatat tttaagtcga tcttttcacc ccgctgatct gtggatcttt
      301 gatgaaattc ataaatataa gacttggagg aattacctta aagggcttta tgacaagctt
      361 ggaaatcagc aaaaaattct cgttaccgga agcgccaagc tggacgttct tcgcaagggg
      421 ggtgacagtc tccagggacg atatcacttc cttaggttga tgccgctgag ttttaaagaa
      481 ctaggaatga ggacaacaaa ggatactcag caacttttcg aactctctgg ttttccagag
      541 cctttcttct ctcagtccaa gagcaaaagc aatcgctggt ctcgcagcta tcgtgagaga
      601 atcatcagac aagaagtggc aaccaacgaa ctgattcaag atttgggaaa tatggagata
      661 ctcctgcacc gacttcctga gttggtgggc gggactctct ccatcaatgc aatttcagaa
      721 gatattcaga tttctcacaa aacgttgtct aagtggatta gtgcgctcga acgtctctat
      781 gctgtttttc gtatcagtcc atttggacca cctaaggtta aagctgtcaa aaaagaacag
      841 aaacttttct tttttgattg gaacgcgatt gttgatccag gcggccgttt tgaaaatttt
      901 gttgccgttc acttattaaa atggatttat tttgaacaag atgtaaatgg aagaaatctt
      961 gatcttcgat tttataagga taaagcgcag cgcgaagtcg actttgttat tattgagaat
     1021 aacagaccta ttttttttat cgaagcgaaa tacggggata gcgagatcac tcaaggtatc
     1081 aaatatctaa aacaaaggtt tcccatggtg cgtgctatgc aagttcatct tcaggggaag
     1141 aaagaatacc aatcagatct tggaatcgaa tatgttcatg tcaaccgcct ccttagcgaa
     1201 ctgatttaac accggaaggg ttgaaaaaaa aggcagtgag ccacccgtaa tgttctgttg
     1261 atacaagaag cgacaggtgg cccgccacca agacggttta aacaacccct ctgcggaaag
     1321 ggcgccaact tttcaggttt ccaaatcgtt taaaacgcag ggaatcccta gttagctaaa
     1381 accgtctaaa taaaaaaacc acaataccaa gcgcttttcc aaaatctgga aaacagcaac
     1441 agagggtgag gtttattggt ggggaattga tgatgaacga tgatttatag cggtggactc
     1501 tccccaattt taaaattttg aacgcaaggt gcgctcctta taagcgaact tgattgaaga
     1561 agaaagagaa gagttttttt ttgtcatttt tttgtaatct tgtcgaatcc ctggcttcta
     1621 tctagata
//
LOCUS       DATBTE010000383         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2101452, whole genome shotgun sequence.
ACCESSION   DATBTE010000383 DATBTE010000000
VERSION     DATBTE010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101452"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VIG33_14320"
     CDS             complement(<1..305)
                     /locus_tag="VIG33_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272062.1"
                     /translation="MRIRTSPTKKTQESENFYSAFNKLDGQHPWMDQVPEGFVAYRVR
                     QLGTGKVAYFNFVLAKEMGLIDSHHPYQMTDELEKKLIEIFSIQIINEYDELTKKRIS
                     "
     gene            472..1104
                     /locus_tag="VIG33_14325"
     CDS             472..1104
                     /locus_tag="VIG33_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272063.1"
                     /translation="MKSFIIIIFSSLVLFTKITQAERKDSGSGLAKLSFTTARNCEGR
                     PDFITEIRPKIIGGFAKLPQIILVANEAEYFIESKNIENGFKVHSYQSFTGTSPRNES
                     KIVCGSTKSNETFRFSLFAPTLIDTTQKRRVGQSLWQFQLLTDKEGFSVWNKRSLAFK
                     DGEDLEKVLMKFGGNYHIYQLSPDQYEVVVSKDTEGITQYLSVKYDAVRK"
     gene            complement(1113..>2079)
                     /locus_tag="VIG33_14330"
     CDS             complement(1113..>2079)
                     /locus_tag="VIG33_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272064.1"
                     /translation="YATELGSLELKDPGNKSICMSYDFHVTEENKLKLIEVNTNASFL
                     ALGYEMYKSRQFPLPVKDFSLEEIRKDVETEIKLCGKIAKSPLTVAIIDEEPEKQRLF
                     IEFLVYQELFKQWGWDSKITDYRKIEPATTDFIYNRYTDFFLSNSESQQLKKAFLDKQ
                     SCFSPNPYEYFLLADKQRMIDWSLSENLTRWNVSAEDQAIIQSVVPFSLSLETHNKED
                     IWAKRKTFFIKPKRAFGSKQSYKGASISRKIFDELAGHEFIAQEFVAPSEQIFKTPEG
                     EQSFKFDLRCLAYQGRLQLIVARLYQGQVTNLRTPHGGFACIEFF"
BASE COUNT          617 a          416 c          426 g          620 t
ORIGIN      
        1 gaaattcttt ttttggtgag ctcatcatac tcattaatga tttgaataga gaaaatttcg
       61 atcagttttt tctctaactc atctgtcatt tgataaggat gatgggagtc gataaggccc
      121 atttccttgg ccagtacgaa attaaaataa gcgacttttc ctgttccgag ttgacgtacg
      181 cgataggcga cgaaaccttc agggacttga tccatccacg gatgctgccc gtccaactta
      241 ttaaacgctg aatagaaatt ttctgattcc tgagtttttt tagtagggga tgtccgtatg
      301 cgcatcgtta cttaagtcct ttgtaaagat ccttagtctt aactatatcg gctaaacgtc
      361 cacgtactta aatgagacat gcataatcta ggtcaaagtc taagactctt tctccgtatc
      421 tttagtgcgt ttgcaaaatg aacgataaga aaaagccgaa ggagtaccac cgtgaaatcg
      481 tttattataa ttatttttag tagcttggtt ttgttcacga aaatcaccca agccgagcga
      541 aaagattcag gctcaggact tgcaaaactc agctttacca cggctcggaa ttgcgaaggt
      601 cggccggatt ttatcactga aatacgtccg aaaatcatag ggggctttgc gaagcttccg
      661 cagatcattc tagtcgctaa cgaagcggag tatttcatag agtcaaaaaa catcgaaaac
      721 ggttttaagg ttcattccta tcagtctttc acaggcacta gccctagaaa tgaatctaag
      781 attgtttgtg gcagtaccaa aagtaatgag acctttcggt tttccctctt tgcccccact
      841 ctgattgata caacacaaaa aagaagagtc ggtcagagtt tatggcagtt tcagttgctc
      901 accgataaag aaggattttc agtttggaat aaacgcagcc ttgcttttaa agacggtgag
      961 gatctggaaa aagttctgat gaaatttggg ggcaactatc acatctatca attgagtcct
     1021 gatcagtatg aagtcgttgt ctcaaaagat accgagggta ttactcaata tctttcagtt
     1081 aaatatgatg ccgtcaggaa atgagcttta tgctaaaaaa attcgataca agcaaagccg
     1141 ccatggggcg ttcttaagtt ggtgacctga ccttgataaa ggcgtgccac aatcagttgc
     1201 aagcgtccct gataagccag acatcttaaa tcaaatttga agctctgctc tccttcaggg
     1261 gtcttaaaaa tttgttctga gggagcaaca aattcctgag cgataaattc atggcccgca
     1321 agttcatcaa aaattttacg actgatagag gcgcctttat aagattgctt cgatccaaag
     1381 gcgcgcttcg gtttaataaa aaaggtttta cgttttgccc agatatcctc tttattgtga
     1441 gtctcgaggc tgaggctaaa agggacgacg gattgaataa tagcctggtc ctcagcgctg
     1501 acattccagc gagtcagatt ttcagatagt gaccaatcaa tcatgcgttg tttatcggcc
     1561 aacaggaaat actcataggg atttggcgaa aaacagcttt gcttatctaa gaaagccttt
     1621 ttaagctgtt ggctttccga attgctaaga aaaaaatcag tataacgatt atagataaaa
     1681 tccgtggttg caggttcaat tttgcgataa tcggtaattt tagaatccca gccccactgt
     1741 ttgaaaagct cctgataaac caaaaactca ataaaaagtc tttgtttttc aggctcttca
     1801 tctatgatgg ccactgtaag gggacttttc gcgattttac cacagagctt tatttcagtc
     1861 tcaacgtctt tacgaatttc ttctaaagag aaatctttca caggaagcgg gaactggcga
     1921 gatttataca tctcgtaacc caaagctaaa aacgaggcat tggtatttac ctctatcaac
     1981 tttaatttat tttcttctgt aacatgaaag tcataactca tgcagatcga tttattgcct
     2041 ggatctttaa gctccaggga cccaagttcc gttgcgtag
//
LOCUS       DATBTE010000384        16607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_736489, whole genome shotgun sequence.
ACCESSION   DATBTE010000384 DATBTE010000000
VERSION     DATBTE010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 16607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16607
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736489"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VIG33_14335"
     CDS             <1..1204
                     /locus_tag="VIG33_14335"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEY8272065.1"
                     /translation="QMTTTVFLQATLDASYNLNSCYALGLADSLWQISPANMADIYYP
                     SGNVGIGTAAPIDKFEVAGSITTSGTAAAVHASSGYFDYSAGVTRIASIGPNATTNGA
                     ISLDTYRSDGSNGIHSFFISSAGNVGIGTINPTAQLFVGSESFAIGTRSSGVFPNNFQ
                     LPGINVSMLGGSYVSAGDSTKTTFLGADVGSGEGMMGTFNNIGLGIRTNNIDRINIDT
                     NGNVGIGIPNPSYQLQLSTDSAAKPGTSTWTIASDERLKDIRAPFDRGLASIEGIHPI
                     YFNYKQGNPLDLPSEKEYVGIKAQDALKVVPEAVSQDDNGYYHITNDAIIWTVLNAVK
                     ELYHKWLDDSSLKDRQIALVIDKASKLEAENAQLKANDSAKDQKIHKLEQENAEIKTR
                     LERIEKML"
     gene            complement(1221..1937)
                     /locus_tag="VIG33_14340"
     CDS             complement(1221..1937)
                     /locus_tag="VIG33_14340"
                     /inference="COORDINATES: protein motif:HMM:NF039851.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5765 domain-containing protein"
                     /protein_id="HEY8272066.1"
                     /translation="MCWNQEISLSIAGAGYIGAYYLKKKTKDPYCWSPILFFASMELL
                     QGLTYFVINDCQNPANRLLTYLSYLHICFQPIFSNIFFMYFLTKEKRMKVIKWVMPIS
                     VLSTVLVLQKLYIYEGCGSCHPEEIFCSDKVCSYFGTWHIGWELILNDNSFYTPEWLN
                     KLYPGKIVLLSPHFISMFLLPLFYGEIGLVVFLFITGPYFAMSVTNDKNEWASIWCLF
                     STTQMSIMLFATRIKHKYLM"
     gene            2058..3326
                     /locus_tag="VIG33_14345"
     CDS             2058..3326
                     /locus_tag="VIG33_14345"
                     /inference="COORDINATES: protein motif:HMM:NF013254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HEY8272067.1"
                     /translation="MKFLFVTRWSSLHVLATQMLKEGHEVKYTVLSRTEKEVGDGFVE
                     KIENWEKYKFWADAIIFDDSDFGEVCEHLRREGCKVVGGTKYSDRLEFDREFGTEEMK
                     AAGLTTIPSWEFQDFESAIQFVKTNPSRYVVKPSGKAQNEKVLSYVGQEEDGKDVITI
                     LERYRKGWGTKIRSFQIQKFVSGVEVAIGAFFNGKKFVMPALVNFEHKKMMNDDIGPS
                     TGEMGTTGFWASDTRLFRETLLKMEPRIGGYVGYFDINCIVNARGIYPLEFTPRFGYP
                     TLNLQLEGVLSKWGDFMYALATGHDFHLRTKKGLQLAVVVAVPPFPFSDDETFRKFSE
                     DAPVVFKRPTTEGVYPCEIKLVDGEWLLAGTSGYSMVVTGAGSTMSDARKEAYSRLKN
                     IQVPNMFYRTDIGERWHRDSDLLQTWGMLT"
     gene            3438..3779
                     /locus_tag="VIG33_14350"
     CDS             3438..3779
                     /locus_tag="VIG33_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272068.1"
                     /translation="MCRFVLLFTVFMTSKISYASELSCNQEITREPYCVSIATAKVYC
                     AKLGSNPETLLKSKKCYVDMTGAGEVITPAGARLTCFLKPVNSSDESLNNSNCGSPAS
                     AASSNYPKGAR"
     gene            complement(3809..5107)
                     /locus_tag="VIG33_14355"
     CDS             complement(3809..5107)
                     /locus_tag="VIG33_14355"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEY8272069.1"
                     /translation="MGSIFNSRIKRALELTKMAAKMGFKEITSGDIQSRIEQAKILTE
                     SLGNLRGAAMKSGQLLSLDLGDYFPPEAVQILAKLQNQVGESPDLDLAQTLKRELTES
                     QRQEIQKIQYKPFAAASMGQVYRAQVANKPIVIKAQYPNLEQSIESDIKMLKRVFSAI
                     CLLSGREMNLDYLFAEIKEVLMQEVDYLKEAEALSKFSTLFQAHEWKFAKVTTPRALP
                     HLSSRRILCLTYEPGLTLKEWIDSNPSKQEREIIARSILELYMMEFFEWGLVQTDPNP
                     GNFLIRRNPELEIVALDFGASKFYSLEFRKNYVELLKSIHSSPPNILVQKSIHFGLLD
                     ARESQDAKLVFIELLKLGMSPFLRKTADDKNTFDFADDTFLKENTRLSKQLIQILKFS
                     PPPHQLIFLHRKLGGIFSILRKLKVELNISEYADKIIGPG"
     gene            5411..5656
                     /locus_tag="VIG33_14360"
     CDS             5411..5656
                     /locus_tag="VIG33_14360"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY8272070.1"
                     /translation="MAKDLLDLHGFKSDEVEDAVDKFLVKVSNSSLKRARIMTGKGTG
                     IVKNLTIRYLKQAGYNWVYEKLPNGKNNEGCLVIFLE"
     gene            5763..6269
                     /locus_tag="VIG33_14365"
     CDS             5763..6269
                     /locus_tag="VIG33_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272071.1"
                     /translation="MNKINLNLLILAFILLPLTAKASCRLGVGEENLTVARVMRNFGS
                     FTLAADSITLRSTNPWGHEETTEVEMGEAIDKLNLAIGCAEEVLKDPTGDVLPSKISS
                     MTDEKEKAELVDDYVYFMTDFKDGLLEYREIFRKLQTQKPANRNFKEAKSKLDDLNEI
                     VDRAHRKL"
     gene            6281..6706
                     /locus_tag="VIG33_14370"
     CDS             6281..6706
                     /locus_tag="VIG33_14370"
                     /inference="COORDINATES: protein motif:HMM:NF019008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HEY8272072.1"
                     /translation="MLKFIFPLFLSLTIVSSSAFAGSVESTENSLKANMKQANTLFKS
                     IGAHINDPTKNTVNAKSAESMAEVFAQVIQQVPKSFDGIPEAQRAEALKGYQTLIQQC
                     IDHSLSLQQAFLEGNNAAALNIYQEMKDLKKEGHEQFDP"
     gene            complement(6943..8592)
                     /locus_tag="VIG33_14375"
     CDS             complement(6943..8592)
                     /locus_tag="VIG33_14375"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8272073.1"
                     /translation="MRILGLFLISMSIILVFASTLRFEFVNFDDYDHLHKNPSVVDFN
                     LERLPKIWTEPHFGLYIPVTYTLWGLEFRFAKWLADLDEDPLSLPSPFIFHGTNVLFH
                     TFNALLVYWLLLQLSLGISAAVIGALIFALHPVQVESVAWISSFRDLLAAFWGLLFLN
                     FTLKTFRDPSALTKGPRTMEGLSLTVLLILAILSKPTAVIFTVFAPLLLYLQEWRPQR
                     RGLGIVNMWFFLWLCLTCTFTAIAKQIQYDANVDLNPQLWERILLSFHSLTFYARSIL
                     WPSQLSPDYGLNPAYLLNMWHATGWPIFTTALSAVLVSAVSGLAWRRHPWAMGLLLAI
                     LGLAPYLGLLSFNFQNVSLVADRYLYLPMIGMSFCFASLPLIPRKKPLGPLKLSSISI
                     LLVMLAIASYQQCAYWRTNEVLYKRMTQLNARSFYGYSGLGGIALLKGENTLAIDLLR
                     KAVELRSSYPPARYNLARALETQGKYLEALQNYVQVINIDPSFIEAYRDLANLLLLQG
                     RLTEAKETVEHALQRDPSSVDLHSLKEEIMNANQKARTNDK"
     gene            8858..9748
                     /locus_tag="VIG33_14380"
     CDS             8858..9748
                     /locus_tag="VIG33_14380"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEY8272074.1"
                     /translation="MASWEELFEKRNQGEKINIPRGLEFNFKNPKTIQEKNILNKIKE
                     FRARQWTQLESELFEQATRFDRAVASLQKKATKKAEQEKAVASRQIARIEKRMENLKS
                     DTLSENESRIYAFDWAPVIILRNNERVIVPMRYHLRPPEMPESFDRKYPGCYNARLDS
                     LTGFWRNEFEKKHGILIISSFFENVKLHQFEKRKLSIKETVKNIVLQYKPKDMEYMVV
                     PCLWDHWGGHGDKAFDSFAIITDKPPPEVLETGHDRCPIFLNEARVEEWLDSKSKSTA
                     ELLSILNDRQRTFYEHKLAA"
     gene            9847..10488
                     /locus_tag="VIG33_14385"
     CDS             9847..10488
                     /locus_tag="VIG33_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272075.1"
                     /translation="MLKLIDLSHIEKSDVSTANSAFEVWKQVYTPILTEANEQIRPEF
                     FWKSKLLCVIEREQLIEGFMLASIYDLSLNGLIDIPYFAPLGDSMKAKLVDDEEKIFT
                     CEWVTVHPLLRGKFSKVQIGDVVMGLGFRAFMDSPCTLAMGFSRTDLKADKMAESFGF
                     ENQGMICRHGIECGIMMLRKSNFKSHRYFKTQQVIEELYANKENKTHLISGDL"
     gene            10485..11141
                     /locus_tag="VIG33_14390"
     CDS             10485..11141
                     /locus_tag="VIG33_14390"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEY8272076.1"
                     /translation="MKKRIESAISEVGATLVSMDFSNQRLYEQWLAQAYFFVKHSTPM
                     LALSAGLSVDDRNYHIRCIDHLSEEKGHDKMLLNDLKFLGRSIDEFQEMASTQALYQT
                     QYYWIEHKSPKSFLGYIFLLEGLAVHIGKAMLDQVSGFKGNSFLRNHAEDDVDHLEKA
                     FLMFDQLTLSEQQAVADNCDLAAAMYISMLQEIKSSLALKKPPLQQSSQTHYTGELLT
                     "
     gene            complement(11062..11613)
                     /locus_tag="VIG33_14395"
     CDS             complement(11062..11613)
                     /locus_tag="VIG33_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272077.1"
                     /translation="MESKKLTQFFNRNEDFSFINRLPESDQLALSVAMEEFAATDTEN
                     YSPSLYSQRLLNRLNLQVDRSIFTRVSKDLEFEFWTAQGKLLGSSNGFYALSSFTLEE
                     LFRKERSQLFQRDQKYTDQLISGIGLALQGTIVENPVEVHTVTEIKINPVSVSVDIKL
                     ITPLYNESGLIVGVAAFLKLKKI"
     gene            complement(11829..12359)
                     /locus_tag="VIG33_14400"
     CDS             complement(11829..12359)
                     /locus_tag="VIG33_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272078.1"
                     /translation="MENTNNLFGINLRKLVDANENSYEEAAETLEISLSFLQQLMRGE
                     RVPSLKTINNVRHKYKVTFNDLLESPLSSEKSIPAKRTVAEMTTEELKELLRPEIRTE
                     IYEAYTLVKKLKEIWGEEGFQAIQRVSKADVLITLDVLAAPYAVAPSSNSGDSQSNQQ
                     IQDGMQKTKSHKFPTR"
     gene            12497..12748
                     /locus_tag="VIG33_14405"
     CDS             12497..12748
                     /locus_tag="VIG33_14405"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8272079.1"
                     /translation="MKIGEFLKQKRLEAGLSQGSMAKRLGYTSPQFISNWERGLSKPP
                     RSKLKKISLVLKINSEQFFDSYIDILLEEYRKDLKKEFK"
     gene            complement(12833..13279)
                     /locus_tag="VIG33_14410"
     CDS             complement(12833..13279)
                     /locus_tag="VIG33_14410"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY8272080.1"
                     /translation="MKLVLNWKEGMRLEAATDGNLISMDAKPPLGKNDGMTPKELVAA
                     GLGGCTGMDVVALLKKHKQAYENFEIGIDITMSTGKTPAVFQRAELTFNLTGPVDKNI
                     LLESVRLSQTKYCGVSEMLLKAFPIRYIVILNNEQIGEGQADFSAN"
     gene            complement(13311..13667)
                     /gene="trxA"
                     /locus_tag="VIG33_14415"
     CDS             complement(13311..13667)
                     /gene="trxA"
                     /locus_tag="VIG33_14415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY8272081.1"
                     /translation="MEILNIETFKSKIFDFEASKDWLFKGPRPAIIDFYADWCGPCRA
                     LAPVLDEVSTQYAGKVDIYKVDTEATPELAALFGVRGIPAILFIPPGGEPAMASGFMP
                     KESFENAINEIFGISN"
     gene            13713..14063
                     /locus_tag="VIG33_14420"
     CDS             13713..14063
                     /locus_tag="VIG33_14420"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY8272082.1"
                     /translation="MRKVKAKKHLAKNLTKNAVSIPKIEGACESVASILKELSHPQRL
                     LILGHLLRGPKTVSQLVKSCGTSQSQMSHFLMRMKYAGLIQSEKAGKFQFYSVADQRL
                     IQLMKTIQNEYCKG"
     gene            14325..15023
                     /locus_tag="VIG33_14425"
     CDS             14325..15023
                     /locus_tag="VIG33_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY8272083.1"
                     /translation="MLQLRKSGDRGFADHGWLKSQHGFSFAEYYDPKFMGFRTLRVIN
                     EDLIEGGMGFGSHPHRDMEIISYVVKGALQHQDSMGTKAMIRPGEVQRMSAGSGVIHS
                     EYNAVPDQETHIFQIWIQPNARGGAPGYEQKSFDSELSAKKLVLVVSGDGRDGSLSIK
                     QDADMYISRLKAEDDLEFKLRPGRGAWVQVVSGSLKINGYELSVGDAAAFENDELLRF
                     KAKDQSELILFDLA"
     gene            15115..15789
                     /locus_tag="VIG33_14430"
     CDS             15115..15789
                     /locus_tag="VIG33_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272084.1"
                     /translation="MLRYRFVLIALLASPLLAEYSLAKDIPVQGRFFAGTTSVDPQNV
                     NETIEAQSLKKMDKVSQFGFEVTYPLLNYLDVGARYTKSLVHNDENPSDINTEYSADL
                     NQDSVLLLARIPFVKSSSVHVDVFAGFGGSNTTFKVKTASQDGEFTRKAVDGWYASPY
                     AAYGASVGVGYKHVFFVIEGGFETNKVDGFTRSGSASTNIETLALSGSYVSIGIMLDG
                     VPGSVK"
     gene            complement(15900..16196)
                     /locus_tag="VIG33_14435"
     CDS             complement(15900..16196)
                     /locus_tag="VIG33_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272085.1"
                     /translation="MTSWAGPFVSEGPGPVETYADCSGQLIDGTAVSFEVRATAAIGF
                     VDSVLIETETNQLIDQLSCQRGSEVVPGVPSAGKIEWICTEYSTTATEWVIRSP"
     gene            16187..16600
                     /locus_tag="VIG33_14440"
     CDS             16187..16600
                     /locus_tag="VIG33_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272086.1"
                     /translation="MKSLQLSPKNGANLTEDQAYRCGKFLQLNNQELDYFLDLVRMDR
                     VADVEVKKYFKSKLEKKSLAAREVKNRVAGSSIEGTLSSQIQYYSSFRRGGDDRDRFL
                     KDGIKFPLKPFAWSCISFPANRDSVPKYFSGRRQS"
BASE COUNT         4794 a         3309 c         3876 g         4628 t
ORIGIN      
        1 acaaatgacg acgacggttt ttttgcaagc gactctggat gcttcctata atctcaatag
       61 ttgttatgcc ctgggacttg cggattcact gtggcaaatc tctcccgcga atatggcgga
      121 tatttattat cccagcggca atgtcgggat tggaacggca gcgccgattg acaagtttga
      181 agtcgcggga tcaataacga catccggaac cgcggcggcg gttcatgctt cgtcaggata
      241 ttttgactat agtgcagggg taacccgaat tgcatcgata gggccaaatg ccactaccaa
      301 tggggccatt agcttggata cttacaggag cgatggttcc aatggcattc attccttttt
      361 catttcaagc gcgggcaacg tcggaattgg aacgatcaat cctacggccc agcttttcgt
      421 cggtagcgaa tcttttgcaa taggcactcg gtcttcaggg gtttttccaa ataattttca
      481 acttccaggg atcaatgtgt ccatgctcgg aggctcgtat gtctcggctg gggattccac
      541 taaaactact tttttaggtg ctgacgtggg ctctggcgag ggaatgatgg gcacatttaa
      601 taatataggt ctaggtattc gaactaataa catagataga ataaatattg ataccaacgg
      661 caacgtcggc attggcatcc caaacccatc ctatcagctt caactttcca ctgactctgc
      721 ggcgaagcct gggacgagta cttggacgat cgcttccgat gaaagactca aggatattcg
      781 tgctccattt gatcgaggct tagcttcaat tgagggaatt catccaattt atttcaatta
      841 caagcaaggc aacccactcg atttaccttc tgagaaagaa tacgttggca ttaaggctca
      901 ggacgcttta aaagtcgttc ctgaggctgt ctcgcaggat gataatggtt actaccatat
      961 taccaacgat gccatcatct ggacggtttt aaacgcagtt aaggagcttt atcataaatg
     1021 gcttgacgac agctcattga aagaccgtca aattgcttta gtgattgata aagcgtctaa
     1081 actcgaagct gagaacgctc agcttaaagc taacgactca gctaaagatc agaagattca
     1141 taagttagaa caagaaaacg cagaaattaa aaccagactg gaacgaatcg agaagatgct
     1201 ttgagatggt taatcatttt ttacattaag tatttatgtt ttatacgagt tgcaaagagc
     1261 ataattgaca tttgagttgt tgaaaaaaga caccaaatgg atgcccattc gtttttgtca
     1321 tttgtcacac tcattgcaaa ataaggtcct gtgatgaata agaacaccac taaccctatt
     1381 tcaccataaa aaagtggaag caaaaacatg gatatgaaat ggggagacaa taatacaatc
     1441 ttaccaggat ataatttgtt gagccactcg ggtgtgtaaa aagaattatc attcaatata
     1501 agttcccaac caatgtgcca agttccaaaa taggagcaga ctttgtctga gcagaaaatt
     1561 tcttctggat gacaggatcc acaaccttca taaatgtata atttctgcaa aactaaaact
     1621 gttgaaagaa cggatattgg catgacccat tttatgactt tcattctctt ttctttggtt
     1681 aaaaaataca tgaaaaaaat atttgaaaaa ataggctgaa aacaaatatg taaataactt
     1741 aaataagtga gaagtctatt tgcgggattt tgacaatcgt taatgacgaa atatgtaagc
     1801 ccctgaagaa gctccatgct cgcaaaaaaa agaatagggg accaacaata aggatctttc
     1861 gttttctttt ttaaataata agcccctata taccccgcgc cagcgataga taatgaaatt
     1921 tcttgattcc aacacatact cttctatctt atcctctaat ggagcgcatt gcgctagcaa
     1981 tagaactctg ttgtgcaatt gtacaacttc tagctcattt aattcccctg tgcggagagt
     2041 aagaataaga tttgggtatg aagttcttat ttgtgacccg ctggtcttca ctccatgttc
     2101 ttgctacgca gatgcttaaa gaggggcatg aggtcaaata cacggtgctt tcccggacag
     2161 aaaaagaggt gggtgacggt ttcgtcgaaa aaatcgaaaa ttgggagaaa tacaagtttt
     2221 gggccgatgc gattattttt gatgactccg attttggtga agtctgcgag catcttcgtc
     2281 gagagggatg caaagttgtc gggggtacaa agtattccga tcggcttgag tttgatcgcg
     2341 aatttggcac tgaagaaatg aaagccgccg gcctgacaac cattccctct tgggaattcc
     2401 aggattttga aagtgcgatc caatttgtga aaacgaatcc cagccgttat gtggtcaagc
     2461 ccagcggcaa ggctcaaaat gaaaaagttc tttcttatgt tggtcaagaa gaggatggca
     2521 aagacgtgat caccatctta gaacgctatc gtaaagggtg gggaacgaag attcgcagtt
     2581 tccaaattca aaaattcgtc tcaggtgtag aagtggccat tggcgccttt ttcaacggaa
     2641 agaagtttgt gatgccagcc ttggtgaact ttgaacataa aaaaatgatg aacgacgaca
     2701 taggtccttc caccggcgaa atgggaacaa ccggattttg ggccagcgat acgagactct
     2761 ttcgcgagac tctccttaag atggagcctc gcatcggcgg ctatgtagga tatttcgata
     2821 ttaactgcat cgtcaatgct cgggggattt atcctctcga gtttactccg cgctttggtt
     2881 atccgacctt gaatttgcaa ctagaaggag ttctttcgaa atggggggac ttcatgtatg
     2941 ccctggcaac aggtcacgac tttcacttaa gaaccaaaaa ggggctgcaa cttgcggtgg
     3001 tggtggccgt gccgccgttt ccattttctg acgatgagac ctttagaaaa ttctcagaag
     3061 atgctccagt ggtttttaaa agaccaacga cggaaggagt ctatccctgc gaaataaaac
     3121 tcgtcgatgg agagtggctt ctggcaggta cttcgggtta ttccatggtc gtgacggggg
     3181 cgggatcaac gatgagcgat gctcgcaaag aagcttattc tcggcttaag aacatccaag
     3241 ttccgaatat gttttaccgt actgatattg gggagcggtg gcacagagat agcgatctgc
     3301 tgcaaacctg gggaatgctg acctagatga ttttcaattt tatttggatc aaaatgagac
     3361 ggagagattc gtttcagtca tatttattgt ttacccttgt aaaatagata ttatttaatg
     3421 atacgaaggg ggaatggatg tgccgttttg tattattatt tacagtattt atgacttcga
     3481 agatttctta tgccagtgag ctcagctgca atcaagaaat aacccgtgaa ccttattgtg
     3541 taagcatagc tactgcgaaa gtatactgtg cgaaattggg cagcaatccg gagactctcc
     3601 taaaaagtaa aaagtgctat gtcgatatga cgggggcggg tgaagttatc accccggctg
     3661 gcgccagatt gacttgcttt ttgaagccgg taaactcatc ggatgaaagc ttaaataatt
     3721 ccaattgtgg aagtcctgct tctgcagctt cctccaatta tccgaagggg gctcgataag
     3781 taaagcttat cttcgtcact gccaattttt atcctggacc tattattttg tcagcgtatt
     3841 cactgatatt caattcgact ttgagttttc ttaaaataga aaaaatcccg cctaattttc
     3901 ggtgaagaaa tatcagttgg tgagggggtg gagaaaactt caggatttgt atgagttgtt
     3961 ttgaaagacg tgtattttcc tttaaaaaag tatcatccgc aaaatcaaac gtatttttat
     4021 cgtccgctgt tttacgcaga aaggggctca tccctaattt caagagttct ataaaaacaa
     4081 gcttagcgtc ctggctttct ctcgcgtcta acagaccgaa atggatagat ttttgaacca
     4141 ggatgttggg aggtgagcta tggatggact taaggagttc tacatagttt tttcgaaact
     4201 caagggaata gaatttggag gcgccgaaat ccagggcgac gatttcaagt tctggatttc
     4261 ggcggataag aaaattccct ggattaggat cggtctgcac taagccccac tcaaaaaatt
     4321 ccatcatata aagttcaaga atagacctgg cgatgatctc gcgttcttgc ttgctaggat
     4381 tcgaatcaat ccattccttc agtgtaagtc ctggctcata ggtgagacat aaaatccttc
     4441 tactgctgag gtggggtagg gcacgggggg tcgttacttt tgcaaatttc cactcatggg
     4501 cctgaaatag agttgagaac ttcgagaggg cctctgcctc tttgagataa tccacctctt
     4561 gcataaggac ctctttgatt tcagcaaaga gataatcaag attcatttct cgtccactca
     4621 gcaggcatat ggccgagaac acccttttca acatcttgat gtcactctcg atcgactgtt
     4681 ccaagttagg atattgggct ttgattacga tgggtttgtt tgcgacttgc gccctataga
     4741 cttgtcccat gctggccgct gcgaagggtt tatactgtat tttttgtatt tcttggcgct
     4801 gagattctgt gagctcccgc ttaagtgttt gcgcaagatc caaatcgggg ctctccccaa
     4861 cttggttttg aagtttcgcc aaaatttgta ctgcttctgg tggaaagtaa tcgccaagat
     4921 cgaggcttaa aagttgtccg gatttcattg cggccccccg cagatttcct agactctcgg
     4981 tgagaatttt tgcctgctct attcttgact gaatatcgcc ggaagtgatt tctttgaaac
     5041 ccatcttcgc cgccattttg gtgagctcga gggctctctt aatgcgactg ttaaagatag
     5101 aacccatggt cactccttta ggagaattga atcttgttgc ctatcttagg tggtgagttc
     5161 tattgagggt ttcatgagct aaagaggaga tcaagacgga aataaggccg agattaaacc
     5221 cgaagtcccc agcttaacgc cgagccgcac ggaaatgccg ctcaagattt ataaaactct
     5281 tcgacaaaaa cgaaatttcc cccactgttt tttagaaaga ttctaatgtt aaacactttt
     5341 ctgaggccga taagggtgga cttggaaaat gaattaaata atattaaaat aaagtttcgc
     5401 aggaggtgat atggccaaag atttattaga tcttcatgga ttcaaaagtg acgaagtgga
     5461 ggacgcggtc gataaatttc ttgtgaaagt atctaattcc agtttgaaga gagctcggat
     5521 catgactgga aaaggaacag gaatagtaaa gaacctgacg attcgatatc ttaagcaggc
     5581 cggctataat tgggtctatg aaaagcttcc caatgggaaa aacaatgagg gctgtcttgt
     5641 tatattttta gaatgattct atcaacttgg gttcttccaa ttgattttca tttactgcaa
     5701 gaatcatcga acttgatgac atccccaaag atttttttta tgattctcat cagagacaca
     5761 ttatgaataa aataaatctg aatctattaa tattagcatt tatcctcctg cccctcactg
     5821 caaaagcgag ttgccgactg ggcgtcggag aggaaaacct cacggtcgct cgagtgatga
     5881 gaaattttgg cagttttacg cttgcggcgg attccatcac tttgaggtct actaatcctt
     5941 ggggtcacga agagactacg gaagtagaaa tgggtgaggc catcgacaaa ttaaatctgg
     6001 ccataggttg cgccgaagaa gtgcttaaag atcctacagg ggacgttctg ccttcgaaaa
     6061 tttcttctat gacggatgaa aaagagaaag cggaattagt ggatgactat gtgtacttca
     6121 tgaccgactt caaggatggg ttgctggagt accgggaaat attccgcaag cttcagacgc
     6181 aaaagccagc gaacagaaat tttaaggaag ctaaatcaaa actcgatgat ctcaacgaga
     6241 ttgtagaccg cgctcatcga aaactttaaa aggaaagctc atgcttaagt ttattttccc
     6301 tctattcttg tctttgacaa tcgtctcctc ttcagctttt gcggggtccg tcgagtcaac
     6361 ggaaaacagc ctcaaagcca atatgaaaca agccaatact ttgtttaaat cgatcggcgc
     6421 tcatattaac gatccgacaa aaaacaccgt gaatgcaaaa tccgctgaga gcatggctga
     6481 ggtttttgcg caggttatcc agcaagtgcc caagtctttt gatggaattc ctgaggcgca
     6541 aagggctgag gctctgaaag gatatcagac tcttatccag caatgcattg atcatagctt
     6601 atcactgcag caggcttttc ttgaagggaa caatgccgct gctttgaata tctaccaaga
     6661 gatgaaggat cttaaaaaag agggacatga gcagtttgat ccttaattaa atagaatctt
     6721 cggttgtcgt tgtcgcgaaa agaggacgct caaaaacagt taattggctt gcgatcgaaa
     6781 aaacaaaagt gcataatcct tctataaaga gaagtgagtc cccggactgg agtgatggga
     6841 agactagaga tctaagccaa agggccgatg atcttgtgga acccccaaaa agtctcaggt
     6901 atcggaggcg gtgaaggaag ctcaacagct tccttcgctt tttcatttat cgttcgttct
     6961 ggccttctga tttgcattca taatctcctc ttttaaagaa tgaagatcga ctgaagaagg
     7021 atctctttgc aaagcatgct ccacagtttc tttggcttcg gttaggcgac cttgaagcaa
     7081 taagagattc gcgagatcgc gataggcttc tataaaagag gggtcgatat taatcacttg
     7141 tacatagttt tggagagctt ctagatattt tccttgtgtc tcgagggctc tggccaagtt
     7201 gtagcgtgct ggaggatagg aactcctgag ttccaccgct tttcggagca aatcaatcgc
     7261 aagtgtattc tcacctttga gcaaggcaat gccccccaag ccactatacc cataaaagct
     7321 tcgtgcgttg agttgagtca ttcttttgta gagcacctcg ttggtgcgcc aataagcaca
     7381 ttgttgatag ctggcaatgg caagcataac cagcaatata cttatgctcg ataatttcag
     7441 gggacccaag ggttttttcc tggggataag tggcaaagag gcaaagcaga aactcatgcc
     7501 gatcatggga agatataaat aacggtcggc caccagcgag acattttgga aattgaagct
     7561 taagagccct aagtagggag caaggccaag tatcgccagt aagagtccca ttgcccaagg
     7621 atgacggcgc caagcgagcc cactcactgc acttacgagc acagcactta gcgcggtggt
     7681 gaaaatgggc cagcctgtgg cgtgccacat atttagaaga taagcagggt tcaagccgta
     7741 gtctggagaa agctgcgaag gccacagaat ggatcgcgcg taaaaggtga gggagtgaaa
     7801 agaaagaagg attctttccc agagctgcgg attgaggtcg acatttgcat catattggat
     7861 ttgttttgcg attgctgtaa aggtacaggt taagcatagc cacagaaaaa accacatatt
     7921 tacaatacct agaccgcgtc tttgtggacg ccattcctgg aggtacaaaa ggaggggcgc
     7981 aaagaccgtg aaaatgaccg ctgtgggctt tgagagtatg gccagtatca ataaaacagt
     8041 gaggcttaat ccttccatgg tcctggggcc cttcgttaaa gctgacggat ctcggaaggt
     8101 ctttaaagta aagtttaaaa ataagagtcc ccaaaaggct gccaataaat cacgaaagga
     8161 tgaaatccaa gccacggatt caacctgaac gggatgaaga gcgaagatta aagctccaat
     8221 aactgccgca gaaattccca ggctcagttg caggagtagc caatagacca acagagcgtt
     8281 gaaggtgtga aagagcacat tggtcccgtg gaaaataaaa ggcgagggaa gcgaaagcgg
     8341 atcttcgtct aaatctgcaa gccatttggc aaatctaaat tccaaacccc acagggtgta
     8401 agtcactggt atatacaatc caaaatgggg ctcggtccag atcttcggga gtcgctcaag
     8461 attgaaatcg acgacggaag gattcttatg caaatgatcg tagtcatcga agttaacgaa
     8521 ttcgaaacgc aatgtggagg caaataccag gatgatagac atggaaataa gaaagagtcc
     8581 cagtatacgc attcctcggg atcttttaaa aaagaaatta ataaaatcct tcgacacatt
     8641 caaatggtgg catggctgaa cagttgaaac aagaggattg ttaactaggt ccatgaggtg
     8701 gcaagatgac caaaaaagga ttcattaacg gaatccctgc taaaactaaa ttcttttcaa
     8761 gctcttcttc catggtacaa tcacttccat gtgttactca gctattgtca aacaggagtt
     8821 aaagcagttg gaggcccggt accaggcgcg ggtagaaatg gcttcgtggg aggaactatt
     8881 tgaaaaacga aaccagggcg aaaaaatcaa tatcccaagg ggtttggaat tcaattttaa
     8941 gaatccgaaa acaatccaag aaaagaatat tctgaataaa atcaaagaat ttagagctcg
     9001 gcaatggacc caattggaat cggagctttt tgagcaggcc acacgtttcg acagagccgt
     9061 ggcaagcttg cagaaaaaag ccaccaaaaa agcggagcaa gaaaaagccg tggccagtcg
     9121 ccaaatcgcc cgtattgaaa aacgcatgga aaatttaaag agtgacaccc tctcggaaaa
     9181 tgaatctcgt atttatgcct ttgattgggc ccccgtgatc attttaagaa ataatgaacg
     9241 agtcattgtg ccgatgcgat atcatttgcg tcctccagaa atgccggaaa gtttcgatcg
     9301 aaaatatccg ggttgctaca atgcgcggct ggatagtttg acaggatttt ggagaaacga
     9361 atttgaaaaa aaacatggta ttttaatcat ttccagtttt tttgaaaacg tgaagcttca
     9421 tcaattcgaa aaacgaaagt tgagcatcaa ggaaacggtg aaaaatatcg ttctccagta
     9481 taagcccaag gatatggagt atatggtggt accttgtctc tgggatcatt ggggaggaca
     9541 tggggataag gcctttgaca gttttgcgat tatcacggac aagccacctc cagaagtttt
     9601 agaaacgggc catgaccgct gtccaatttt tttaaatgaa gcacgcgttg aggaatggtt
     9661 ggattccaaa agtaaatcaa ctgcggaact tctgtctatt ttaaatgacc gccagcggac
     9721 attttatgaa cataagcttg cggcttagtg aagcttattc tagttgctgg aattttcgaa
     9781 ttaaaagcag gtccagttta tattaagact cttgtcgagc tgataaattt atgaggaaat
     9841 tggcgaatgc taaaactcat agatctttcg cacattgaaa agtctgatgt gagcactgct
     9901 aattccgctt ttgaggtttg gaagcaggtt tatacgccca tcctgactga agccaatgag
     9961 caaataagac cagagttctt ttggaaatct aaactgttgt gcgtgataga gcgagaacag
    10021 ttgatagagg gttttatgct ggcgagtatc tatgatcttt ctttaaatgg cttaatcgat
    10081 attccctatt ttgcccctct tggtgattcg atgaaagcca agctggtcga tgatgaggaa
    10141 aagattttta cctgtgaatg ggtgacggtt catccccttc tccgcggaaa gttctctaaa
    10201 gttcagattg gggatgtggt catggggttg ggcttcaggg cgtttatgga ttcgccctgc
    10261 acattagcca tggggttctc gcgcacggat cttaaagccg ataagatggc agagagcttt
    10321 ggttttgaga accaaggaat gatctgcagg catggaattg agtgcggaat tatgatgctg
    10381 cggaaatcaa actttaaatc ccatagatat tttaaaactc agcaagtgat tgaagaactc
    10441 tatgcgaaca aagaaaataa aacccattta atctcgggag acttatgaaa aagaggatcg
    10501 aaagtgccat cagcgaggtc ggtgccacac ttgtgtctat ggatttttcc aatcaaagac
    10561 tctatgaaca gtggttggct caagcttatt tctttgtaaa gcattctact ccgatgcttg
    10621 ccttgtcggc gggcctctct gttgatgatc gtaattatca tatccgctgt atcgatcact
    10681 tatccgagga aaaaggccat gataagatgt tattaaatga cctcaagttt ttaggaagaa
    10741 gtatcgacga atttcaagag atggcctcca ctcaggcttt gtaccaaaca caatattatt
    10801 ggatagagca caaatcgcca aaatcgtttt taggatatat atttttgctc gaaggactcg
    10861 ccgttcacat cggcaaggcc atgttggatc aggtctccgg ttttaagggg aatagctttt
    10921 tgaggaatca tgccgaagac gatgttgatc atctcgagaa agccttttta atgttcgatc
    10981 agctgacgct cagtgagcaa caggcggttg cggataactg tgatttagcc gcggcaatgt
    11041 atatttccat gcttcaggag attaaatctt ctttagcttt aaaaaagccg ccactccaac
    11101 aatcaagcca gactcattat acaggggagt tattaactta atatcaaccg atacgctgac
    11161 cggattaatc ttgatttctg tgactgtatg aacctcaacg ggattttcca caatggtccc
    11221 ttgcaaagcc aggccaatac cagagatcag ctggtcagta tacttctggt ccctttgaaa
    11281 aagttgcgat ctttcttttc tgaaaagttc ctcaagggtg aacgaagaca atgcgtaaaa
    11341 cccattagag ctgcctaaaa gttttccttg ggctgtccaa aattcaaatt ccaaatcttt
    11401 tgaaacccgg gtgaatatgg atctgtcgac ctgtaaattt aatctgttaa gcaatcgctg
    11461 ggagtacaga gaagggctat agttctctgt atctgtggct gcaaactcct ccattgccac
    11521 tgataaagcc aattgatcgg actctggcag tcgatttatg aatgagaagt cctcgttgcg
    11581 attaaagaat tgagtgagtt tttttgattc catttcgcgt aagtataaca ggttttagag
    11641 taaatttata tctgttggcg aagtttgaaa aaaattcgag gagttggggt tgacgtttaa
    11701 aagttagaca attaaaagta cgtttatgaa aaatgtctca ctctttaagt cttgttcctt
    11761 actgagtcta attcaggcga aaaatatacc ctcggataag cttgtgagtt acttgagaca
    11821 gctaggcctt agcgagttgg aaatttgtga ctttttgttt tttgcattcc atcctggatc
    11881 tgctggtttg attgagaatc gccggagtta cttgacggag ccacagcgta aggggcggcc
    11941 aggacatcta aggttattaa aacatccgcc tttgaaactc gttgaatagc ctgaaacccc
    12001 tcttctcccc atatttcttt tagtttttta acgagagtgt aagcttcata aatttctgtt
    12061 ctgatttctg gcctgagaag ttccttaagt tcttcggttg tcatttccgc aacggttcgt
    12121 tttgcaggaa tcgatttttc agatgagaga ggggattcaa gaaggtcatt aaaggtcacc
    12181 ttatatttgt gacgaacatt attgatggtt tttagggagg ggactctttc tccacgcatt
    12241 aattgctgca agaaagacag ggaaatctcc aatgtctcgg cggcctcctc gtagctgttc
    12301 tcatttgcat cgactaattt tctaaggttg atgccgaata aattattcgt attttccata
    12361 acttacgccg ctttctcata aaacgtctta atataaacat gacgaagtgt aaataaaaat
    12421 aagctatatg tgtatttata tgtagaccaa tatatgccat atgtgtattt tttataagtg
    12481 atgggaaaag aggactatga aaatcgggga atttctaaaa cagaaaagac tagaagcagg
    12541 tctctctcaa gggagtatgg ccaagcggct tgggtatacg agccctcagt tcattagtaa
    12601 ttgggagcgt ggactttcta agccgccgag atctaagctt aaaaagataa gcttagttct
    12661 gaaaattaat agcgagcaat tttttgattc atatattgat attcttcttg aagaatatcg
    12721 caaagatctt aagaaagaat tcaaataatt aaaaacggct gacttcaatt tcaccgcgac
    12781 gctttttttc aggggtggac agaacgacaa cgtcgccttc aatcccttac tgctaatttg
    12841 cggaaaaatc cgcctggcct tcgccgatct gttcattgtt gagaattaca atatagcgaa
    12901 tagggaaagc ctttagcaac atttcactga ctccgcagta tttcgtctgg gaaagtcgga
    12961 ccgactccag taaaatattt ttgtcgacag ggccggtgag attaaaagtc aattcagccc
    13021 gctgaaaaac agcgggcgtt ttccctgtcg acatcgtgat atcgattcct atctcaaagt
    13081 tttcataagc ttgtttatgt ttttttaaaa gggctaccac gtccatgccg gtgcatccgc
    13141 caaggcctgc ggcgaccaat tcctttggcg tcatgccgtc gtttttaccg aggggaggct
    13201 tcgcatccat agagatcagg tttccatcgg ttgcagcttc aagacgcatt ccttctttcc
    13261 agtttaatac aagcttcatg tcgactcctt attgtcaggt gttatttagg tcaatttgag
    13321 atgccaaaaa tttcattaat tgcattttca aagctttcct tcggcataaa cccagaggcc
    13381 atagcgggtt ctccaccggg agggatgaag aggatggcgg gaattcctcg gactccaaag
    13441 agagccgcca gctcaggtgt ggcctcagta tccaccttgt atatatccac tttaccggca
    13501 tactgagttg aaacttcatc cagtacaggg gctaaagcgc ggcaagggcc acaccagtcc
    13561 gcgtaaaagt cgataatggc aggtcttggt cctttgaaga gccaatcttt tgaggcttca
    13621 aagtcaaata tcttggattt aaacgtttct atattcaaga tttccatgaa aatctccttt
    13681 tctttaatat atgattatat cataatatgt caatgcgtaa ggtaaaagca aaaaaacatt
    13741 tagctaaaaa tttaacaaaa aatgctgtgt ccattccaaa aattgagggt gcctgtgaat
    13801 cggtggcgtc tattctgaag gaactttctc atccgcagag gttattgata ttagggcatc
    13861 tgctcagggg tcccaagacg gtttcgcaat tagtgaagtc ctgcggaact tcacaatcgc
    13921 agatgtccca ttttttaatg agaatgaagt atgcaggact gatacaatca gaaaaagccg
    13981 gaaagtttca gttttactcg gtggcggacc agcgattaat tcagctgatg aagacgatac
    14041 agaatgagta ttgcaagggt tgagtgttga tggagggcat gggggggagt tattagaagc
    14101 atcatttttt ttctggttct gttatttaaa acaatcgctg cggctcagga gccgtcttcc
    14161 acactacgtc caccatcgat ggatttatgt atataatatc aaactgcggg atgccagaag
    14221 agttaacttt acaatgtcga agttccgagc gggtaattga aagatctctg cggtaatatt
    14281 aagataggag aaggagaaac aggtgtaatc ataagggagg ttgtatgtta caactcagaa
    14341 aatcagggga cagaggcttt gcggatcatg gatggcttaa atctcagcat ggtttttcat
    14401 ttgccgaata ctatgatccg aaatttatgg gttttcgcac tctgcgtgtg atcaacgaag
    14461 acttgattga aggtggaatg ggttttggtt cgcaccctca tcgcgatatg gaaattattt
    14521 cttatgtcgt gaagggagcg cttcagcatc aagattcgat gggaaccaaa gccatgatcc
    14581 gtcccggcga ggtgcaaaga atgagcgcag gttccggagt tattcactcc gagtacaatg
    14641 ccgtgccaga ccaagaaaca cacatttttc agatttggat tcaacccaat gctcgcggtg
    14701 gggctccggg ttatgagcaa aaatctttcg actccgaact cagtgcgaag aagttggtac
    14761 tggtggtttc tggcgatggc agggacggtt cgctgagtat aaaacaagat gctgatatgt
    14821 atatttctcg cttgaaggcg gaagacgacc ttgaatttaa actccgtcca ggcagaggag
    14881 cctgggttca ggtcgtgagc ggatcactaa aaattaacgg ctatgagctt tcggtagggg
    14941 atgcggcggc gtttgaaaat gacgagcttt taaggttcaa ggcgaaggat caaagcgaac
    15001 ttatcttgtt tgatctggca tagcactttg gtcgggatga aggttgcgaa tcgagctcta
    15061 gacttgagat gatgttttat aaaacatatt atgaacattg tcttgaggtg ttatatgttg
    15121 agatatcgtt ttgttcttat agctcttctt gcgtcgcctc tccttgcgga atactcctta
    15181 gcaaaggata ttccggttca gggtcggttc tttgccggca ccacaagcgt ggatccacaa
    15241 aatgtcaacg agactataga ggcccagagt cttaagaaga tggataaagt ttctcagttc
    15301 gggtttgaag tgacttatcc gcttttaaat tatttagacg tcggcgcccg ttacacaaag
    15361 agtcttgtcc acaacgatga gaatccttcg gatatcaaca ccgagtacag tgcggattta
    15421 aatcaggact cagtgttgct tttggcgagg attcccttcg tgaaatccag ttcagtgcat
    15481 gtggatgttt ttgcgggctt tggtggcagc aataccacgt tcaaggtgaa gaccgccagt
    15541 caagatgggg aattcactcg caaggccgtt gacggctggt atgcctcgcc ttatgcggcc
    15601 tacggtgcct ccgtaggcgt cggctacaaa catgttttct tcgtcatcga aggcggcttt
    15661 gagacaaata aagtggatgg tttcactcgc tccggttccg caagcaccaa tatcgaaacc
    15721 cttgctcttt ctggttccta cgtttcaata ggcatcatgc tcgatggagt gccaggaagt
    15781 gttaaatagc tagctatttt attatttaca gattaatgat ccaacaactt tagggggcaa
    15841 cggaaacatt tgctcttgtt taatctggcc agtggtagct ccaacaacgc ctccagacat
    15901 taaggtgacc gaataaccca ttctgttgcc gttgtgctgt actcagtgca aatccactct
    15961 atctttccag cggagggaac tcctgggact acctcactgc ctcgctggca agaaagttga
    16021 tcaatcaatt gattcgtttc agtctcaatc aatacactgt cgacaaagcc gatagccgca
    16081 gttgcgcgaa cttcaaaact gacggcggtg ccatcaataa gttggccact gcaatcggca
    16141 tatgtttcca caggtcccgg accttcgctg acaaaggggc cagcccatga agtcattgca
    16201 gctgagtcct aaaaatggag cgaatttaac cgaagatcag gcctatcgat gcggcaagtt
    16261 tctgcaatta aacaatcaag agctcgacta tttcttagat ttggtacgta tggatcgtgt
    16321 tgctgatgtt gaagtaaaaa aatattttaa gagcaagctt gaaaagaaat ctttagccgc
    16381 acgtgaagtt aaaaatcgtg tggctggatc ctccatcgaa gggactttgt cctctcaaat
    16441 tcaatactat tcaagcttcc gaagaggtgg cgatgatcgt gatagatttc ttaaagatgg
    16501 gataaaattt cctctaaaac ctttcgcttg gagttgcatc tcatttcctg ccaacagaga
    16561 ttcagttcca aaatattttt ctggtcggag acaatcttga ttttcca
//
LOCUS       DATBTE010000385        15315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_53040, whole genome shotgun sequence.
ACCESSION   DATBTE010000385 DATBTE010000000
VERSION     DATBTE010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 15315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15315
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53040"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1389
                     /gene="metK"
                     /locus_tag="VIG33_14445"
     CDS             226..1389
                     /gene="metK"
                     /locus_tag="VIG33_14445"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEY8272087.1"
                     /translation="MKNYLFTSESVSEGHPDKMADQISDGILDAILAQDPKGRVACET
                     LLTTGLVVVAGEISTAAKVNFTDIARNVIKRIGYDHSDKGFDYKTCGVMVAVGQQSPD
                     IAVGVKETLSDDQGAGDQGLMFGYAVNETPELMPLSIAMSHTLVRELAALRKANKVNW
                     LRPDAKSQITVQYENGLAKRIDTIVISTQHAEDVKNETIKDFVVNELIKKHIPAKWID
                     NNTKFHVNPTGRFVLGGPMGDAGLTGRKIIVDTYGGHGAHGGGAFSGKDPSKVDRSAA
                     YAARHIAKNIVGAGLADRCLIQVAYAIGVAEPVSITVNDYGTSKVGVEALEKAVRKVW
                     DLRPARITKEFELLRPIYSATSAYGHFGRNEEGFTWEKLNKVDQLKEVVKSLS"
     gene            1448..3247
                     /gene="lepA"
                     /locus_tag="VIG33_14450"
     CDS             1448..3247
                     /gene="lepA"
                     /locus_tag="VIG33_14450"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_038451587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEY8272088.1"
                     /translation="MDPKFIRNFSIIAHIDHGKSTLADGLLQATGALTDREKKAQFLD
                     SMELERERGITIKAQTVCLNFKSKDSNTYQLNLIDTPGHVDFSYEVSRSLAACEGAIL
                     VVDAAQGVEAQTLANVYLAIENNLTIIPVLNKIDLPSADPEGVRKQIEETVGLDCTGI
                     IHASAKEKIGITDILEAIVEKVPPPTADRNLTPRALIFDSWFDAYQGVVILVRVMDGS
                     IKKGDRIKFMATDRDYEVLRMGKYTPFPDAQDVLDAGEVGFIICGIKDIRDVKVGDTI
                     TASKHPSQIPLPGFKKIKPMVFAGIFPIVASEYESLRDALDKLCLNDSSLTFEVEKSM
                     ALGFGYRCGFLGLLHMEIVQERLEREFNLDLITTAPTVVYRITKTDGSILMLENPSGM
                     PPETQIEKFEEPFVRVTVHTPTEYIGGILKLCEDKRGIQQKMDYVTEKKVIIEYKLPM
                     NEMVMDFYDRLKSISKGYASLEYEFLDFEEADLVKMDILINGDPIDALSLIVHRSKCI
                     NRGRALTEKMKELIPRQQYQVAIQAAIGAKIVARETLGALRKDVTAKCYGGDISRKRK
                     LLEKQKEGKKRMKQVGNVEVPQEAFLAILKVED"
     gene            3249..4016
                     /gene="lepB"
                     /locus_tag="VIG33_14455"
     CDS             3249..4016
                     /gene="lepB"
                     /locus_tag="VIG33_14455"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY8272089.1"
                     /translation="MSTEKKNSWDFRSKYFWTDGWGSIFLAVFIALFIRWGFIEAYVI
                     PSGSMLPSLLIHDHIFVNKLVYGIRVPFSENWLIKFGTAKRGEVIVFKYPKDMSTFFI
                     KRVVGEPGDKIYYENGTLFINDKPQEKKVPVSQDDFNWLRDADFQRDGNLGDDKNNYV
                     FFTEALGDKDHSMLLRRGDIYESYGPVTVPEGQLFVMGDNRNNSADSRVWGFLPEQNI
                     LGRAMFIWLSCEDTIPGIPVLCNPLTIRWGRFFHQVN"
     gene            4020..4736
                     /gene="lepB"
                     /locus_tag="VIG33_14460"
     CDS             4020..4736
                     /gene="lepB"
                     /locus_tag="VIG33_14460"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY8272090.1"
                     /translation="MSRGVKKSLKGTWPQAIGTFFFPLFIVFILRWLVIEPFVIPSGS
                     MIPNLLVHDHILVKKYSLGLKIPFSDHWFVRWKAPRRGEVLVFRYPENPDVFYIKRLI
                     GLPGDVVVVRGSGQITINGQEWTLQAQDSFQNSSQEDNFKYYLEDTGEQKHIVRFYGS
                     PLASNESQEYRVPQQEYFFMGDNRDQSSDGRVWGFVPEKYLVGPASVIWLSCENTLTS
                     VSYICDPRQIRWKRLFNSVQ"
     gene            4687..5442
                     /gene="lepB"
                     /locus_tag="VIG33_14465"
     CDS             4687..5442
                     /gene="lepB"
                     /locus_tag="VIG33_14465"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY8272091.1"
                     /translation="MIQDKFAGKDYLIQYNSSMLSSQWKDYLVTILISILLALFVRSF
                     IITAYKVPTGSMQPVLKAGDFIFSYRMAYGFKLPWSQIAWGEKTPDRGDVVVFSYANQ
                     TNTSYVKRVIGLPGDKIQITGGKLIVNEVPLDYELEHSSSENAFYREDNPNPESFDIY
                     LERDLGNTRRVIFQKNRSDQDFGPLVIPPGEVFLLGDNRDASDDSRHWGTVPIGQVYG
                     KVLFVWLSLDWQKPWGGDRYPGIRWERVFFGVH"
     gene            5454..6410
                     /locus_tag="VIG33_14470"
     CDS             5454..6410
                     /locus_tag="VIG33_14470"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY8272092.1"
                     /translation="MTRSGGDQYSLLQMSQPFSTLIEIANLDFVKIEDLFLSALEFSK
                     NPAAQNFAAKTAALLFFEPSTRTRFSFETAAARLGLHPLVLDGVNGTSLEKGESIEDT
                     VLNIAAMQPSAIVIRCGDEVDLYSLQEKAKIPILNAGWGKRAHPSQALLDALTLKANG
                     RDLKKEKLLVIGDIKHSRVMGSHFQLAKILGYEIGMCGPKEFLPENNSSGIHFTNLTD
                     GLSWATSVMALRVQHERHGKDHSIDNYQANYPNQYGLSLQKLKAWKSDGLIMHPGPVN
                     YGVELSLDVNQDPRCKILEQVKWGTYLRQALLAGVLSAKAVS"
     gene            6407..7474
                     /gene="carA"
                     /locus_tag="VIG33_14475"
     CDS             6407..7474
                     /gene="carA"
                     /locus_tag="VIG33_14475"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEY8272093.1"
                     /translation="MKKIFLVLETGEVYSGKWHGGENRAGEVVFNTSHSGYEEIATDP
                     SYFNQIVVMTAPMQGNYGIDMKSWESTRLWIEGFITLQLQESTRDRAWVQCLSENKIP
                     VASEFDTRELVLRLRSGGTPWGALVQSDNEADAKRKAFSLIDEKKRLEKDWVYLASRK
                     TKESRRGDFLGGPKVAVLDFGSKENILRELQRYCSELVIYPSRATAQELLSSNPDFVM
                     LTNGPGDPAAVQVAPQTIRELVGKKPIFGICMGHQVLSLALGAKTYKLKFGHRGANHP
                     IKDDLLGQIYMTSQNHGYAVDPQSLPAEARVTQINLNDGTVAGFYNPEKKYLGIQYHP
                     EACPGPHEAKNLFKFFIEKVL"
     gene            7471..8118
                     /locus_tag="VIG33_14480"
     CDS             7471..8118
                     /locus_tag="VIG33_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272094.1"
                     /translation="MNEYEKLIKKILDHFAGDNFKAELIRAKKEFFENSGTLDEHSEY
                     YELRMSQFYDWYFFTRELSGYGQSPLNACLLERELRFSEEELKQLAVLKQNRHSVFEF
                     NKIKGTDVYIKDLLANKKVVVKSSPWIFGFAEEELFEARLIPCGNNFIFTRGFCFHPE
                     LAKKFILDEVKRHRKDPDLDPDYMMLRFIKMRFKYEQYRHVNPEMIYTNDGKLGL"
     gene            8200..11397
                     /gene="carB"
                     /locus_tag="VIG33_14485"
     CDS             8200..11397
                     /gene="carB"
                     /locus_tag="VIG33_14485"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEY8272095.1"
                     /translation="MPRRADIKRVLIIGSGPIVIGQACEFDYSGTQACKALMKEGLEV
                     ILVNSNPATIMTDPEGATRVYVEPLKVDYLEKIIAKERPDAVIPTLGGQTALNLALEL
                     HKHGILEKYKVELLGATPKVIEAAEDREIFRSILDKVGAKYPKSHMVRTYEQGQQVAE
                     VLGYPLILRPNYTLGGGGGGIAYTPEAYQKMLVGALHESPTSEVLVEESILGWKEYEL
                     EVMRDAAGSFVVICSIENLDPCGVHTGDSITVAPQQTLSDQEYQAMRDEAYKIINEVG
                     LATGGANIQFATHPTTGERVVIEMNPRVSRSSALASKATGFPIAKIAAMLAIGYRLDE
                     IRNDITKSTPSCYEPALDYVVTKIPYFAFEKFPGATDSLNTQMKSVGEVMGIGRTLQE
                     SLMKALASLEKNPQGIPEMVLSIDKVSYPNSQRIYHLFQIFREGKSVAEVEALTQINP
                     WFLEQIEGLVVFERKFKTEFFTDNKDLLLEAKRKGFTDARLGVLVGKTEQEIRELRDK
                     YDLHPRYQQVDTCAGEFESSTPYFYSSYWFNKSVPLKVKDPVVLIGSGPNRIGQGIEF
                     DYSCVRGVKAFQKTGHPVIMVNSNPETVSTDYDTSDVLFFEPLTAESLIEVMRYTKPM
                     GFVTQLGGQTPINLAPKLQKAGFKILGSTLETIELAEDRGLFSKICRELDLKIPNSAM
                     VGNIEEALAQESHVGYPMIARPSYVLGGRRMEVVENREELQSYFERHREFISKDRPCM
                     IDQFLLGALEVDVDLVRGADWTLIGGIVEHIEAAGVHSGDSMGVLPPQRLRESTCERI
                     ELLSIQLAERLKVIGHLNLQLAVKNDAIYVLEANPRSSRSVPFIAKATGIPLIDLGVA
                     AVCGVPKSQLKLENLQWRDTSTVSVKGVVFPFKKFSEADSILGPEMKSTGESMGRGSD
                     YAEALMKAFISSNVHLPQKGEVFFSLRDKDKESLLGLARVLMKMGYTISATAGTAKYF
                     NQKGVHCLALKKVDEGRPHCVDRIRSGHVSLVINTTAGRRAIEASFDIRRACTDYSIP
                     CLTESDAAEAFVLALRKVRTGQSDVQPLKATEVFN"
     gene            11430..12170
                     /locus_tag="VIG33_14490"
     CDS             11430..12170
                     /locus_tag="VIG33_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8272096.1"
                     /translation="MAILQVKALNKTFAGGLFEKPRHVLKDVTFSLPAGETTGFVGSN
                     GSGKTTSIKCMLDFMRPDSGEILFFGATLGLSAKKRIGYLPERPYLYEFLSGMEFLRL
                     HWDLVQNSRKDFIERAHEALKRVNLYDARDKKLRQYSKGMLQRVGIAQAILNRPDLLI
                     LDEPMSGLDPDGRGLVKDILREESKRGTSLFFSSHLMQDMEELCSHLVIINKGAILYD
                     GDLLPFMKQYASLEDAFRFATEQGRETL"
     gene            12167..12928
                     /locus_tag="VIG33_14495"
     CDS             12167..12928
                     /locus_tag="VIG33_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY8272097.1"
                     /translation="MRKVFVIAVTTLREMIRERFFLIIVFIAVAFFGVSLLLGALSFG
                     EQQRILVNLGFTAIEVASVGISLFAGAFLISKEIEKQTLLLVLARPISRDQFLMGKIS
                     GILVLNTLLVWALGTVLYFLLGTRSDFLNFTTILFSIWFESVITLSLVIFFSIVVRPI
                     LSLMMTFGILMLGEWIPDLQFFAEKSKDHFFINAVKVVTWITPNLFRFNWKSYYFLEL
                     GFDGQDIVWMIAHSFGWALIVFVLACLLFRRKDIV"
     gene            12921..13712
                     /locus_tag="VIG33_14500"
     CDS             12921..13712
                     /locus_tag="VIG33_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY8272098.1"
                     /translation="MSELDVFFYLFFFLLGACFGSFSNVIIYRMPKGESVVKPRSRCQ
                     KCGKNVAWYDNIPLLSWFLLRGKCRHCGAKFSFRYFFVEFLTAFLFSLAYFYLGLTWN
                     LLEALIFIFPLVCCTFIDFDHMILPDEFTLSGVVIGLLGAWLNPQREFLDALIGTFMG
                     GGFLWAMAYLYYVFTKQDGMGGGDIKLLAWIGAVLGWKAVPFVIMTSAVIGSVVGLIL
                     ARKQKQGLKTMIPFGPYLALGAVLYMFGGQTIALWYFNLFLPAFS"
     gene            13827..14885
                     /gene="pilM"
                     /locus_tag="VIG33_14505"
     CDS             13827..14885
                     /gene="pilM"
                     /locus_tag="VIG33_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEY8272099.1"
                     /translation="MFFKSKKVLGLDIGTSSIKIAELDVSRKGAQLVSFGFAPTPPNS
                     VAAGEISDVSSVGAAVQGLLSEVNSKRKNISTGMWGTAVIVKKITIPKMDQKLIQDQI
                     RFEAEQYIPFDINNISLAHHVLSTSSSEDTIDLLLIAAQNELVFQYTAVVESAGLKCS
                     VLDVSGFALANCFEANYGKLTRDVVGILNFGASITNFVVIYKGDVVFCRDVPVGGQNY
                     TNEIHKSMGISRHEAEALKLSAVSRRGEVPAEVHSIIHSTNEIVTDEIRNSLDFLSAT
                     TNGLTLNHCFYTGGSSTTTGLIENVSRVTGIRFDPFNPFTKVTANSKKFSPDYLNQIS
                     HFAAIATGLGLRQSGDSQ"
     gene            14882..>15315
                     /locus_tag="VIG33_14510"
     CDS             14882..>15315
                     /locus_tag="VIG33_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial assembly protein"
                     /protein_id="HEY8272100.1"
                     /translation="MIRINLASIRTMAAATRGTIEGDGAGEVVISEEGRKDALVKLLV
                     ILIAPMALYLYENQNIPTIRNELNRKLASLAELQAFNAKAEDSVKEIKKFKEDEKKIQ
                     TRIAVLEKIAKDRFREVKVLDLFQQVIPEKLWFTRVEIKEGKV"
BASE COUNT         4062 a         3047 c         3690 g         4516 t
ORIGIN      
        1 aaatcgagca attaaactct cgtttggagt ttgaagctaa gagcggaaag aaagtgtcgt
       61 aacctctgta gtgcttccgc agtaagcact cattattaaa gcccccggtc taaaaaatcg
      121 ggggcttttt attgaccctt atcttggttt gcgttaattt gcgccgttat aagaaaattc
      181 aaacaattgt caaactaaga ccattctaag agtgaggacg aaaaattgaa aaactatctt
      241 ttcacaagtg aatctgtttc tgaaggacac ccagataaaa tggccgatca aatcagcgat
      301 ggcattttag atgccatctt agcgcaagat ccgaaaggtc gcgtcgcttg tgaaaccctg
      361 ttgactactg gtctcgtcgt ggttgcagga gaaatctcaa ccgctgcaaa agtaaatttc
      421 acagatattg cccgtaacgt catcaaacgc attggttatg atcattctga taagggtttt
      481 gattataaaa cttgtggtgt gatggtcgct gttggccaac agagcccgga tattgcagta
      541 ggcgtaaaag aaacattgag cgacgatcaa ggtgccggtg accaaggctt gatgtttggt
      601 tatgcagtga atgaaactcc agagttgatg cctctttcaa ttgcgatgtc tcacacttta
      661 gtccgtgaat tggctgcttt gagaaaagct aataaagtaa actggctccg tcctgatgcg
      721 aaatctcaga tcactgtaca atacgaaaac ggtcttgcaa aacgtatcga cacgatcgtg
      781 atttccactc agcatgcgga agacgtaaaa aacgagacta tcaaagattt cgtcgtgaac
      841 gagttgatca aaaaacacat tccagccaag tggattgata ataatacaaa atttcacgtg
      901 aatcccacag gccgtttcgt tttgggtggc ccaatgggtg atgcgggttt aacaggtcgt
      961 aaaatcatcg ttgataccta tggcggtcat ggcgcccatg ggggcggtgc tttctctggt
     1021 aaagatcctt caaaagtaga ccgctccgct gcttatgcgg cacgtcatat tgctaagaac
     1081 attgtaggag ctggacttgc agatcgttgt ttaatccaag ttgcctacgc gattggtgta
     1141 gctgagcctg tgagcattac agtgaatgac tatggaacaa gcaaagtcgg agttgaggct
     1201 ctggaaaaag cggttcgtaa ggtttgggat ttacgtcctg ccagaattac gaaggaattt
     1261 gaacttcttc gtcctattta cagtgccact tccgcttatg gccatttcgg acgcaatgaa
     1321 gaaggtttca cttgggaaaa actgaacaaa gttgatcaat tgaaagaagt cgtaaagagt
     1381 ctttcataaa aattgttagt tggctgtctt aaggacagag tgcctataga aggcagaatc
     1441 gggcgaaatg gatcctaaat ttatacgaaa tttctcaatc attgctcaca ttgaccacgg
     1501 gaagtccact cttgcagatg gacttctcca ggcaacgggg gcgctgacgg atcgagagaa
     1561 aaaagctcag tttttggata gcatggagct tgagcgagag cgtgggatca cgattaaagc
     1621 tcagactgta tgtctgaatt ttaaatctaa agacagcaat acttaccagc tgaacttgat
     1681 cgacaccccg gggcatgtgg acttttctta cgaagtgtct cgttctcttg ccgcctgtga
     1741 aggggcgatt ctggtggtgg atgcggctca aggcgttgaa gcccaaactc tggccaatgt
     1801 ttatttggct attgaaaata atctgaccat cattcctgtc ttgaataaaa ttgatttgcc
     1861 ttctgcggac cctgaaggcg tgcgtaaaca gattgaagag actgtcgggc ttgattgtac
     1921 gggcattatc catgcttctg ccaaggagaa aatcggtatt accgatattc tcgaggccat
     1981 tgttgaaaaa gttccgcctc ccacagcaga tcgtaatttg actccgcggg ctttgatttt
     2041 cgactcctgg tttgatgcct atcagggcgt ggtgattctg gttcgcgtga tggatggttc
     2101 catcaaaaaa ggcgaccgga ttaaattcat ggctacggat cgtgattatg aggtcctgcg
     2161 tatggggaag tacacgccgt ttccagatgc tcaagatgtt cttgatgctg gagaggtggg
     2221 gttcattatc tgcggtatca aggatattcg cgacgtgaag gtgggtgata ctatcactgc
     2281 ttcaaaacat ccctcacaga ttccattgcc gggttttaaa aaaatcaagc cgatggtctt
     2341 tgccgggatt tttccgattg tggcttctga atatgaaagc ctccgcgatg ctctggataa
     2401 attgtgttta aatgactctt ctttgacctt tgaagttgag aagtccatgg ctctgggctt
     2461 cggctatcgt tgtggtttct tggggcttct gcatatggag atcgtgcagg aacgtcttga
     2521 gcgtgagttc aacttggacc ttatcacgac agctcccact gtggtttaca gaattactaa
     2581 gacggatggt tcgatcctga tgctcgagaa tccttcagga atgccgcctg aaacgcaaat
     2641 tgagaagttt gaagaacctt tcgtaagagt gaccgttcat actccgaccg agtatattgg
     2701 tggaatcctc aaactttgcg aagacaagcg tggcattcag caaaagatgg actatgtaac
     2761 cgagaaaaaa gtcatcattg agtacaagct tccgatgaat gaaatggtaa tggactttta
     2821 tgatcgtttg aagtctattt ccaagggcta cgcttcttta gaatatgagt tcctggattt
     2881 tgaagaagct gatttggtaa aaatggatat tttaatcaat ggggatccta ttgatgcttt
     2941 atccttaatc gtccatagat ccaaatgcat taatcgtggg cgtgctttga ccgaaaaaat
     3001 gaaagagctg attccgcgcc agcagtatca agtagcgatc caagcggcta tcggagccaa
     3061 aatcgtggct cgtgagacct taggggcgct tagaaaagac gtgacggcga agtgttatgg
     3121 tggtgatatt tctcgtaagc gtaagctttt agaaaaacag aaggaaggta agaaacgcat
     3181 gaagcaagtc ggcaatgtcg aagtgcctca ggaagcattt cttgcgatcc tcaaagtgga
     3241 agattaatat gagtacagag aagaagaata gttgggattt tcgcagcaaa tacttttgga
     3301 ccgatggatg gggatccatc ttccttgcgg tttttattgc tttattcatt cggtggggat
     3361 ttatcgaagc ctatgtgatt ccttcaggaa gcatgttgcc gtccttacta attcatgatc
     3421 acatcttcgt taataaactt gtttatggga tccgcgttcc ttttagtgaa aattggctta
     3481 ttaaattcgg aactgcgaaa cgtggtgaag ttattgtctt taaatatcct aaagatatga
     3541 gcacattttt tattaaacgt gttgttgggg agcctggcga caagatttat tatgagaatg
     3601 gaactctctt tatcaatgac aaaccccaag agaaaaaggt cccggtcagc caagatgatt
     3661 tcaattggct tcgtgatgcg gattttcaac gtgatggaaa cctcggcgat gataaaaaca
     3721 actatgtttt cttcacagag gccttggggg acaaggatca cagtatgctg cttcgtcgtg
     3781 gcgatattta tgaaagctac ggtcctgtta cggttccaga aggtcagctt tttgtaatgg
     3841 gtgacaatcg taacaactcg gcggacagcc gagtgtgggg attcttacct gagcaaaata
     3901 ttttgggtag ggctatgttt atttggttga gctgtgaaga tacaatccct ggtattccgg
     3961 tgctttgcaa tccattgacg attcggtggg gtcgcttctt ccatcaggtg aattagaaga
     4021 tgtctagggg agtaaaaaaa agtcttaaag gaacttggcc ccaggccatc gggacttttt
     4081 ttttccctct attcattgtc tttatcctgc gttggcttgt cattgaaccc ttcgtgattc
     4141 cctccggcag tatgattccc aatcttttgg ttcatgatca tattctagtg aaaaaatact
     4201 ccttgggttt aaaaattcca ttttccgatc actggtttgt tcgttggaaa gctccccggc
     4261 gtggagaggt tctagtcttt cgttatccag aaaatcccga tgtattttac attaagcgct
     4321 tgattgggct tcctggcgat gtggtcgtgg tgagaggcag tggacaaatc acaatcaatg
     4381 ggcaagagtg gactttgcaa gctcaagatt cttttcagaa ttcttcgcag gaagataatt
     4441 ttaaatacta tcttgaagat acaggtgaac agaaacatat tgttcgcttt tatggctctc
     4501 cattggcaag caatgaaagc caagagtatc gagttccgca gcaggaatac ttttttatgg
     4561 gggataatcg cgatcagtcc agtgacggac gcgtctgggg ttttgttccc gaaaaatacc
     4621 ttgtcggtcc cgcttcagtc atttggctta gctgtgagaa caccctgact tcagtgtctt
     4681 atatttgtga tccaagacaa attcgctgga aaagattatt taattcagta caatagttct
     4741 atgttgagca gtcagtggaa ggattacctg gttactattc taatctctat cttgttggcc
     4801 ttgtttgtaa gaagcttcat aataaccgct tacaaagtac cgacgggctc gatgcaaccg
     4861 gttctcaaag ctggtgattt tattttttct tatcgtatgg cctacggctt taaactccct
     4921 tggagtcaga tcgcttgggg agaaaagacc cctgatcgtg gcgacgtcgt cgttttttct
     4981 tacgcgaatc agacgaacac aagctatgta aagcgggtga ttgggctacc aggagacaaa
     5041 atccaaatta ccggaggaaa acttatcgtc aatgaagttc ctctggatta tgaacttgaa
     5101 cattcttcct cggaaaacgc cttctatcgg gaagacaatc ctaatcctga atcctttgat
     5161 atttaccttg aaagagacct tggtaacact cgtcgggtga tttttcagaa aaatcgcagc
     5221 gatcaggact ttggtccctt ggtgattccc ccgggagaag tctttctctt aggggataat
     5281 cgtgacgcca gcgacgactc tcgacactgg ggcaccgtgc caattgggca agtttatggc
     5341 aaagttcttt ttgtctggtt atctctggat tggcaaaagc cttggggcgg agaccgttat
     5401 cccgggattc gctgggaacg agtgtttttc ggagtgcatt agactcgtct tcattgacac
     5461 gttcaggggg tgaccaatat tctctgctgc agatgtctca gccattctct accttaattg
     5521 aaattgccaa cttagatttt gtaaaaatag aggatctttt tctttcggcc ctggaatttt
     5581 caaaaaatcc agcagcgcaa aattttgctg caaaaactgc ggccttgcta ttttttgagc
     5641 ccagcactcg cacgcgtttt agttttgaaa ccgccgccgc tcgtcttgga ctccatcctt
     5701 tagtattaga cggcgtaaac gggaccagcc ttgaaaaagg tgaaagtatt gaagacacgg
     5761 ttttgaatat cgcagcgatg cagccttccg caatcgtcat tcgttgcgga gatgaggtcg
     5821 atctttattc tttgcaagaa aaagcaaaga ttccgatttt gaatgccggt tggggtaaac
     5881 gagctcatcc ttcgcaagcc ttgttggatg cgctgacttt gaaagccaac ggtcgcgact
     5941 taaaaaaaga gaagctatta gtcattggcg atataaagca cagtcgcgta atgggttctc
     6001 acttccagtt ggctaagatc ttaggctacg aaattggaat gtgcggtcct aaggaatttc
     6061 ttccagagaa taattcttcc ggtattcact tcacgaatct tacggatggt ctttcctggg
     6121 cgacctcggt gatggcttta agagttcaac atgagcgcca tggcaaagat cactccatag
     6181 ataattatca agctaactat cccaatcagt atggattgag tttacaaaag ctcaaagctt
     6241 ggaagtcgga tggtttgatc atgcatcccg gacctgtgaa ctatggagtt gaactcagtt
     6301 tagatgtgaa tcaagatccc cgttgcaaaa ttctagagca agtaaagtgg ggaacttatt
     6361 tacgccaggc cttgctcgct ggcgttctct cggcaaaggc ggtctcgtga aaaaaatatt
     6421 tctcgtcctg gaaaccgggg aagtttactc tggaaaatgg catggtggtg aaaatcgtgc
     6481 cggagaagtg gtgtttaata cctcccattc aggctatgag gaaattgcca ccgacccttc
     6541 ttattttaat caaatcgtgg tcatgacggc tcctatgcaa ggaaactatg gcattgatat
     6601 gaaatcttgg gagtccactc gtctgtggat cgaagggttt attaccctgc aactccaaga
     6661 gtccacccgg gatagggctt gggttcagtg tttaagtgaa aataaaattc ctgtagcctc
     6721 tgagtttgat acccgcgagc tagttctgcg cctgcgttca ggcggaactc cctggggagc
     6781 actggttcag tcggataatg aagccgacgc taaaaggaag gcttttagtt taatcgacga
     6841 aaagaagaga ttagaaaagg attgggttta tttagctagt cgaaaaacca aagaatctcg
     6901 tcgcggtgat tttttaggtg gcccaaaagt agcagtcctt gattttggct ctaaagaaaa
     6961 catattgcgg gaacttcaaa gatattgttc agaactcgtg atttatccca gccgtgcgac
     7021 agcgcaagaa ttgctttcta gcaatcctga ttttgtgatg ctcaccaacg gtcccggcga
     7081 tccggcggct gtgcaagtgg cgccgcagac aattcgtgaa cttgttggta aaaaaccaat
     7141 atttggtatt tgtatggggc atcaggtttt gtctttagct ttaggcgcca agacctataa
     7201 acttaagttt ggccatcgtg gagccaatca tcctattaag gatgatctct tgggacagat
     7261 ctatatgact agtcaaaacc atggttatgc cgtagatcct caaagtttgc cggcagaggc
     7321 ccgcgtcact cagattaatt tgaatgatgg aaccgtggcg gggttttata atcctgaaaa
     7381 gaaatattta ggaatccaat atcatcctga agcctgccct ggtcctcatg aggcaaaaaa
     7441 cttatttaag ttttttatag agaaagtttt atgaacgagt acgaaaagtt aattaaaaag
     7501 attttggatc actttgcggg agacaacttc aaagcggaac ttattcgtgc caaaaaggaa
     7561 ttttttgaaa actcggggac tttggatgaa cattcggaat attatgaatt gcgcatgtct
     7621 cagttctacg attggtattt cttcactcga gagctgagtg gttatggcca aagccctttg
     7681 aatgcctgct tgcttgaacg cgagctgcgc ttttcagaag aagagcttaa acaattggca
     7741 gttttaaagc aaaatcgtca ctctgttttt gaatttaata aaatcaaagg gacagacgtt
     7801 tacattaaag atctcttagc gaataaaaaa gtcgtggtga agtcctctcc ttggattttc
     7861 ggttttgccg aagaagagct gtttgaagct cgcttgattc cctgtggtaa taactttatt
     7921 tttacccgtg gcttttgttt tcatccggag cttgctaaaa aatttatttt ggatgaagta
     7981 aagcgccatc gtaaggatcc agatttagat cctgattata tgatgcttcg gtttattaaa
     8041 atgcgtttta aatacgagca atatagacat gtgaatcccg aaatgattta taccaacgac
     8101 ggcaagctgg gattatagag ctcgctgata ttgctaaaga cattgcaatt aatttgcaaa
     8161 tacactgtta cttttaatgt gtaattaaag gagtcctcct tgccgcgcag agctgatata
     8221 aaaagagttt tgattattgg cagtggaccg attgttatcg gtcaagcctg tgaatttgat
     8281 tactcaggca ctcaggcctg taaggcattg atgaaagagg gccttgaggt cattcttgtt
     8341 aattccaatc cggccacgat catgacagat ccagaaggag ccacccgcgt ttacgtggag
     8401 ccccttaaag tggactacct ggaaaaaatt atcgctaagg aacgaccgga tgcggtcatt
     8461 cccaccctag ggggtcaaac agctttgaat ttggccttag agcttcataa acatggaatc
     8521 cttgaaaaat acaaagtgga attactcgga gccactccga aggtgatcga agctgcggaa
     8581 gatcgtgaga tcttccgcag cattcttgac aaagtagggg cgaaataccc taagagccac
     8641 atggtccgta cttatgagca gggtcagcaa gtggcggaag ttctcggtta tcctctgatt
     8701 cttcgcccca attacacttt gggcggtggt ggcggcggca ttgcctatac tccagaagcc
     8761 taccaaaaaa tgctagtggg tgcccttcat gagagtccca cttctgaagt tttggtggaa
     8821 gaaagtattc tgggctggaa agaatatgaa cttgaagtga tgcgagacgc agcgggatct
     8881 tttgtggtga tctgcagtat cgaaaatctg gatccttgcg gagttcacac cggagacagc
     8941 atcacagtgg ctcctcagca aactctcagt gatcaagaat atcaagccat gcgcgatgaa
     9001 gcctataaaa ttattaacga agtgggtctc gcaaccggtg gtgcgaacat ccagtttgcc
     9061 actcatccga ccacaggtga gcgggtggtg attgaaatga atccacgcgt gagccgctcc
     9121 tcagctttag cgagtaaggc cacggggttt cctattgcta aaattgccgc gatgttggcc
     9181 attggctatc gcttggatga aattcgcaac gacatcacca agagcactcc ttcttgctat
     9241 gaaccggccc tcgactacgt cgtaaccaaa attccgtatt ttgctttcga aaaattcccg
     9301 ggagctacag acagtctgaa tacgcaaatg aagagtgtcg gtgaagtgat gggaatcggc
     9361 cggacgctgc aggagtcttt gatgaaggct ctggcctctc tggaaaagaa tcctcaaggt
     9421 attcctgaga tggttctttc aattgataaa gtgtcttatc cgaacagcca aaggatttat
     9481 catctcttcc agattttccg ggaagggaag tccgtcgcgg aagtggaagc tttgacgcaa
     9541 attaatccat ggttcttaga gcagatcgaa ggcctggtcg tttttgaaag aaaatttaaa
     9601 acagaatttt tcaccgataa taaagacctg cttctagaag caaaacgaaa aggttttacc
     9661 gacgcccgct taggagtgct tgttggtaaa actgaacaag agatacggga gctgcgtgat
     9721 aagtatgacc ttcatccgcg ttatcaacag gtggatacct gtgccggtga atttgaatcc
     9781 tccactccct atttttattc ctcttattgg tttaataaaa gtgtgccctt gaaggtcaaa
     9841 gaccctgtgg tgttgattgg cagtggtcca aatcgcattg gccaaggaat tgaattcgat
     9901 tattcctgtg tccgtggagt taaggctttt caaaaaactg gtcatcctgt gatcatggtg
     9961 aactcgaatc cagagactgt ttctacggat tatgacacct ctgatgtttt gttttttgaa
    10021 cctctgacag cggagagcct cattgaagtt atgcgctaca caaagcccat gggttttgtg
    10081 actcagctag gtggtcaaac tcctatcaac ttagcgccta agctgcaaaa ggccggcttt
    10141 aagattttgg gttccaccct tgagacgatt gagcttgctg aagatcgcgg actctttagt
    10201 aagatctgcc gggaactaga cttaaaaatt ccaaactctg ccatggtagg aaatattgaa
    10261 gaggccttgg cccaagaaag ccatgtcggt tatccgatga ttgcacgtcc aagttatgtt
    10321 ttggggggtc ggcgaatgga agtggttgaa aatcgagaag agcttcaatc ttattttgag
    10381 cgccatcgag aattcatttc taaagatcga ccgtgtatga ttgatcagtt cttgctcgga
    10441 gctttggaag tcgatgtgga ccttgtaaga ggagcggatt ggactttgat cggcggaatc
    10501 gttgagcata ttgaggccgc gggtgttcac tccggcgaca gcatgggagt tttacctccg
    10561 caacgtcttc gcgaatccac ctgtgagcgt attgagcttt tgagcattca attagctgag
    10621 cgcctcaagg tgattggcca tttgaatctc caattggcgg ttaaaaatga cgctatttat
    10681 gttttagaag cgaaccctcg cagctctcgt tcagttccct ttattgcgaa ggccacaggt
    10741 attcccttga ttgatctggg agtcgctgcc gtatgtgggg tgcctaaatc tcagctaaaa
    10801 cttgagaatc ttcaatggcg agacacatcc accgtgtccg tcaaaggagt ggtttttcct
    10861 ttcaaaaaat tctcggaagc tgattcaatt ctcggacctg agatgaagtc taccggcgaa
    10921 agcatgggtc gcggatctga ttacgccgaa gctttgatga aagcctttat ttccagcaat
    10981 gtgcatctgc cccaaaaagg agaggttttc ttctccttac gtgacaagga taaagaaagc
    11041 ctcttgggct tagctcgggt tttaatgaaa atgggttata ccatctccgc aactgctgga
    11101 accgcgaaat actttaatca gaaaggtgtt cattgtctgg ctcttaaaaa agtggatgaa
    11161 ggacgtcctc attgtgtgga tcgcattcgc tcagggcatg tcagtttggt tatcaatacc
    11221 acagcaggtc gtcgcgccat cgaagccagc tttgatatcc gacgtgcctg tacggactat
    11281 agcattcctt gcttaaccga gagtgatgcc gcggaagcct tcgttttggc tctacgaaaa
    11341 gtgcgaacag gacaaagtga tgttcagccg ttgaaggcga ccgaagtctt taattgaaaa
    11401 tccagtgaat acgaatagaa tgatgccata tggctattct tcaagtaaaa gcattaaata
    11461 aaacatttgc gggggggcta tttgaaaagc ctcgccatgt tctaaaagat gttacttttt
    11521 cccttcctgc aggggaaacc acgggtttcg tgggaagtaa tggctctggg aaaaccacgt
    11581 ctattaaatg catgctggat tttatgcgcc cggattcagg ggaaattctt ttcttcggcg
    11641 cgaccctcgg tctttcagca aaaaagcgta ttggctatct tcctgaacgt ccttatctct
    11701 acgagttttt gagtggaatg gagttcttgc gtctccactg ggatttggtc cagaattctc
    11761 gaaaagattt tatagagcga gcccatgagg ctcttaagcg cgtaaattta tatgatgctc
    11821 gcgataagaa gcttcgccaa tactccaaag gaatgttgca aagggttggc attgcccagg
    11881 ctattttaaa tagaccagat ttattaatcc ttgatgagcc gatgtccggt ctagatcccg
    11941 atgggcgcgg attggtcaaa gatatccttc gcgaagaatc taagcgaggg acgagcttgt
    12001 ttttcagtag tcacctcatg caagacatgg aggagctttg ttctcatcta gtcatcatta
    12061 ataaaggagc gattctttat gatggagatc tgctgccctt tatgaagcaa tatgcaagct
    12121 tagaagacgc tttccgtttt gctacagagc aaggaaggga aactttatga ggaaggtttt
    12181 tgttatcgca gtgaccaccc ttcgagaaat gattcgcgag agattcttcc tgattatagt
    12241 tttcatagcc gtggcttttt ttggagtaag tcttttgttg ggagctctct ccttcggtga
    12301 gcaacaacga atcttagtca atttgggatt taccgcgatt gaagtcgctt cagtagggat
    12361 ctctctcttt gctggagcgt ttttgatttc aaaggaaatt gaaaagcaga cattattgtt
    12421 ggttttggct cgtccgattt cccgcgatca attcttgatg ggaaaaattt ctgggattct
    12481 tgtcctcaat accttgttag tttgggccct ggggacggtt ttatacttct tgcttgggac
    12541 aaggtccgat tttttgaact ttacaacaat cttattttcg atctggtttg agtccgtcat
    12601 taccctgtct ctcgtaattt tctttagcat tgttgtacgt cctatcttat ccttgatgat
    12661 gacttttggg attttgatgc tgggggagtg gattccggac ttgcaattct ttgctgagaa
    12721 aagcaaagat catttcttca ttaatgcggt gaaagtagtc acctggataa ctccgaattt
    12781 atttcgcttt aactggaagt cttattattt tttagaatta ggatttgatg gacaggacat
    12841 agtctggatg attgcccact ccttcggttg ggccctgatt gtttttgttt tagcttgctt
    12901 actttttagg agaaaagata ttgtctgaac tggatgtttt tttctattta tttttctttt
    12961 tactcggagc ctgcttcggc agtttttcca atgtaatcat ctatcgtatg cctaagggtg
    13021 aaagcgtggt gaaaccgcga agtcgctgcc agaagtgcgg taaaaatgtg gcttggtatg
    13081 ataatattcc gctgttgtcc tggtttcttt tacgtggaaa atgtcgtcat tgcggagcta
    13141 aattttcctt ccggtatttt tttgtagagt ttctcacggc ctttttgttt agtttagctt
    13201 atttttatct tggcttaact tggaatctac tggaggcgtt gatttttatt ttccctctcg
    13261 tttgctgtac ctttatcgat ttcgatcaca tgattttgcc agatgagttt acgctctcag
    13321 gtgttgtgat cggccttctt ggcgcgtggt taaatccaca gcgtgaattt ttagatgctc
    13381 ttataggcac cttcatgggc ggcggatttt tgtgggcgat ggcttatctc tattatgttt
    13441 tcacaaagca agatggtatg ggcggaggag acatcaagct tttggcttgg atcggcgctg
    13501 tcctgggatg gaaagcggtt cccttcgtga tcatgacttc ggctgtgatc ggaagtgtag
    13561 ttggtttaat cttggcgaga aaacaaaagc aaggcttaaa gacgatgatt cccttcggtc
    13621 cgtatctagc cctaggtgca gttctttata tgtttggcgg tcagaccata gccttatggt
    13681 attttaattt atttctccca gcctttagct agactcaccc gtttgacatt cgtgtctcca
    13741 gaagaaataa ttttaatagt tagaatttag gctggtgcct aaacatttga ttttagacca
    13801 gtgtctgaaa tggtggctgg aaaaacatgt tttttaaatc gaaaaaagta ctgggactcg
    13861 acattggcac gagttctatc aaaattgctg agttggatgt ttccagaaag ggagctcagt
    13921 tagtctcttt cggttttgct ccaactcctc caaattcagt agccgctggc gaaatctctg
    13981 atgtctcttc cgtcggggcg gccgttcagg ggcttcttag tgaagttaat tcgaaaagaa
    14041 aaaacatttc caccggaatg tggggaaccg ctgttattgt taagaaaata accattccta
    14101 agatggatca aaaactgatt caggatcaaa ttcgctttga agcggaacaa tatattcctt
    14161 ttgatattaa caacataagt cttgctcatc atgttctgag tactagttct tctgaagata
    14221 ctattgattt acttttaatc gctgctcaaa acgaattagt ttttcagtac acggcggtgg
    14281 ttgaatccgc aggactcaaa tgcagtgttt tggatgtgag tggctttgca ctcgcaaatt
    14341 gttttgaagc taattatgga aagcttactc gcgatgtcgt tggaatcctt aactttggtg
    14401 cctccatcac caactttgta gtcatctata agggggatgt ggtcttttgt agagatgtcc
    14461 cggttggcgg tcaaaactat acaaatgaaa tccataaaag tatgggaatt tctcgacacg
    14521 aagctgaagc tttaaagctc agtgctgttt ctcgtcgggg tgaggtgccg gcggaagttc
    14581 acagtattat tcactctact aacgaaatag tcactgatga gattcgtaat agtttggact
    14641 ttttaagtgc cacaacaaac ggtttaactt taaatcattg tttctataca ggtggcagct
    14701 caacgaccac gggtctcatt gaaaatgttt ctcgagtgac gggaattcga tttgatcctt
    14761 ttaacccttt taccaaggtc actgccaatt caaaaaagtt ttcacctgat tatcttaatc
    14821 agatcagcca ttttgctgct atcgctacag ggctgggtct tcgccagtcg ggagatagtc
    14881 aatgattcgt attaacctcg cttcgatacg aacaatggcg gctgccacaa gagggaccat
    14941 tgaaggagat ggtgccggtg aggttgtaat ttctgaagag gggcgtaagg atgcacttgt
    15001 aaagcttttg gtgattttaa tcgctccaat ggcattgtac ttatacgaaa atcaaaatat
    15061 tccaaccatt cggaatgagt tgaatcgcaa gctagcatct cttgcagagc tgcaagcttt
    15121 taatgccaag gctgaagatt cggtaaaaga aatcaagaaa tttaaagaag atgagaagaa
    15181 aatccaaact cgtattgcag ttttagagaa aattgctaag gatcgtttcc gtgaggtgaa
    15241 agtcctggat cttttccaac aagtaattcc tgaaaaactt tggtttacac gggtagaaat
    15301 caaagaaggc aaagt
//
LOCUS       DATBTE010000386         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1623826, whole genome shotgun sequence.
ACCESSION   DATBTE010000386 DATBTE010000000
VERSION     DATBTE010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623826"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VIG33_14515"
     CDS             complement(<1..849)
                     /locus_tag="VIG33_14515"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEY8272101.1"
                     /translation="MRKRDWFLVTLPLVITWLVDRVSKIATTGLQGTVTHGPVSFILH
                     HNHGAMLGLFSDLPAVLRIVSLSTGGAFLLCSYVIVQYLLPIRSLTLRSGLSILLGGI
                     LGNVADRIIYGYVIDFIVLGSPSLSSPAFNLADALQWVGYGMIIFAIVREGDLLWPEH
                     NARRQYWINRKFQLKYCLILMAVGLSLTMVSLVFSYTYLRVTIAELVGNNQFLLNKFL
                     VPFVLTYASICMAFCAILFAVGRLISHRIAGPIYAFERFLMDALEGKERALKLRSGDE
                     FKHLEKL"
     gene            929..>2301
                     /gene="dacB"
                     /locus_tag="VIG33_14520"
     CDS             929..>2301
                     /gene="dacB"
                     /locus_tag="VIG33_14520"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEY8272102.1"
                     /translation="MKYLLFVCLLTSGLSISVSASAEENLKTELQKLIKHSGIRESDL
                     GLYITMGEGENQEVVMDLNSKRKMIPASISKVATTSAVLDYFPPGFKFKTQLLSASPV
                     REKVLKGDLILKGGGDPSFVSENMWFLVNNFKRNEIQKIEGDIVVDDSLFDQKRYDSS
                     RQPERVDRAYDAPVGAMSFNWNSINIFVRPGEKSGQPGKVFLDPENDYVHLVNKTTTN
                     GGSENSLVASRVEDKNFQGDVIHVSGKIGKDSKEIAIFKNITQPDLWAGYNLKSFLNQ
                     RGISVTGKIRTGKVPEKAVILAESESKGVEQSLQDMNKFSNNYVAEMFTKQLGALKAQ
                     PGSLETGMEMIQAHLKKIELPEDQYYFQNPSGLTRDNRISAFAMWKIIHHLRKDFQVQ
                     PEFLASLPIAGIDGTLKKRMKNSPGERWVRAKTGFLTGVVSLAGYAGRSDGRVFSFTF
                     IFNGTKD"
BASE COUNT          703 a          473 c          551 g          574 t
ORIGIN      
        1 aagtttttcc aagtgtttaa attcatctcc tgaccgcagc tttaaggcac gctccttgcc
       61 ctctaacgca tccatcagaa atctttcaaa agcataaata ggtcctgcta ttcgatggga
      121 aatcaatctg ccaacagcaa agagaatggc acagaaagcc atacaaatgc tcgcataagt
      181 gaggacaaag ggcaccagga acttatttaa taggaattga ttattgccca ccagttccgc
      241 aatggtgact cgcaagtaag tgtaagaaaa aaccagactg accatagtga ggctgaggcc
      301 caccgccatc agaatcaaac aatatttgag ttgaaatttt ctattgatcc aatattgtct
      361 gcgggcattg tgttcaggcc ataaaagatc accttcgcgg acgatcgcaa aaatgatcat
      421 gccataaccg acccattgaa gcgcatcagc cagattaaaa gccgggctcg aaaggcttgg
      481 tgaacccaaa acaataaaat cgatgacgta accatagata atgcgatctg caacgttgcc
      541 aagaattcca ccgagaagga tcgagagccc cgaacgcaat gtcagactgc gaatgggcag
      601 taagtactgt acaatcacgt aggagcagag gaggaaagcg ccccccgtgg aaagagaaac
      661 aatccgcagc accgccggta agtccgagaa aagtcctaac attgcgccat gattgtggtg
      721 aagaatgaaa cttactggac catgggttac ggttccttgt aggccggttg ttgcaatttt
      781 acttactcgg tcaacaagcc aagttataac taaaggcaga gttactagga accagtcgcg
      841 ttttctcata tcttttcaat gtatcaagtg attttccttg agagcaacaa ggcacatcga
      901 taccattttt tagtctaggg agatttttat gaagtacctt ttatttgttt gccttttaac
      961 ttcgggactt tcgatttctg tttcggcttc cgctgaggag aatttaaaaa cagagcttca
     1021 gaagctgatt aagcactcag gaattcgaga atcggactta gggctctata tcactatggg
     1081 tgaaggtgaa aaccaagaag tagtgatgga tctgaactct aaaagaaaaa tgattccggc
     1141 ctccatctcg aaggtcgcaa cgacgtcggc agtgctggac tattttcccc ccggtttcaa
     1201 attcaagacc caactgctgt cagcaagtcc agttcgagaa aaagttctca aaggagatct
     1261 cattctgaaa ggcgggggag atcctagctt tgtttcagaa aacatgtggt ttttggtgaa
     1321 caatttcaaa cgcaacgaga ttcaaaaaat tgaaggcgat attgtggtgg atgattcact
     1381 ttttgaccaa aaaagatatg attccagccg tcagccagaa cgggtcgatc gcgcctacga
     1441 tgctccagta ggggcgatgt cctttaactg gaattcaatc aatattttcg tgcggccagg
     1501 cgaaaaaagt ggacaaccag ggaaggtctt tttagatcca gaaaatgatt atgtacatct
     1561 ggtgaataag acgaccacca atgggggcag cgaaaatagt ttggtggctt cgcgggtcga
     1621 ggataaaaac ttccagggtg atgtgattca cgtgagtgga aagatcggca aggattccaa
     1681 agagatagcg atttttaaga acatcactca accggattta tgggcgggct ataacctcaa
     1741 gtcttttttg aaccagagag gcatttccgt gacaggaaaa attcgcacag gcaaagttcc
     1801 ggagaaggcc gtgattcttg cggagtccga gagtaaaggg gtggaacaat ccctccaaga
     1861 tatgaataag ttttccaata attatgtagc cgaaatgttc accaagcagt tgggggcttt
     1921 gaaagcacag ccagggagtt tggaaacggg aatggaaatg atccaagctc atttgaaaaa
     1981 aatcgagctt cctgaagatc aatattattt tcaaaatcct tctggcttaa ctagggacaa
     2041 tcgcatcagt gcttttgcga tgtggaagat cattcatcat ttgcggaagg actttcaagt
     2101 tcaaccggaa tttttagctt cgctgcccat tgccggaatc gatggcactc tgaagaagcg
     2161 aatgaaaaac agtcccgggg aacgatgggt cagggcaaaa accggatttt tgaccggagt
     2221 tgtgagccta gctggttacg ctggacgtag tgatggtcga gtttttagct tcacctttat
     2281 cttcaatggt acaaaagatg a
//
LOCUS       DATBTE010000387         7257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2307488, whole genome shotgun sequence.
ACCESSION   DATBTE010000387 DATBTE010000000
VERSION     DATBTE010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2307488"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VIG33_14525"
     CDS             <1..552
                     /locus_tag="VIG33_14525"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY8272103.1"
                     /translation="GNIPVIAAGGIYTHQDILKYIDMGCAGVQMGTRFLATHESGANA
                     EYKKMLVECKEEDIELASRPGSPCGMLFHVIKQSPFYQQAVARERAPKCDKGYLLNKG
                     HCPSKYENEKTFCICNGLLSSINLNNKDEKDLYTVGANAYRIDRILSVHELMAELQDI
                     PLSEASSGASCLKQETCVHQKII"
     gene            578..1054
                     /gene="greB"
                     /locus_tag="VIG33_14530"
     CDS             578..1054
                     /gene="greB"
                     /locus_tag="VIG33_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEY8272104.1"
                     /translation="MDQSKNYITPSGVAKLKAEYHELMHVERPKVVEVVTWAAGNGDR
                     SENADYQYGKKRLREIDKRVRFLIQRIDKAEVIDPKTIDSATVFFGATVKIMNEEEQV
                     MTYQIVGEDEFDIKLGKISWKSPLARALLGKKVGDEAVVHKPNGEEYITIEEIQYK"
     gene            1055..2356
                     /locus_tag="VIG33_14535"
     CDS             1055..2356
                     /locus_tag="VIG33_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272105.1"
                     /translation="MVHFKSLTPFLFFLLISQTGSFAKSETQGKVSFLGISRPELKSD
                     FAESADFVVELEGEHKYYFNKNLSFQVSPWVRSNWASKDSVQKFFLEAQEIAISYKKS
                     SQKVMIGFHRQIWEGTDLVNPMSLQNPQFYLDPLVTKARSSAGLFYSDQIGSFSWDFS
                     YIPKQGKSVLPGEHSAWWPRSLNLPLQTESMEVRLPEDIAYQINNDEVLDRALDHNVG
                     FRLMINLNSLDVAMAGFEGSSQAPLLIPVLTMNPIEVSPRQVFQVTSPIAITPTYFRR
                     RAMAGVMTWSLNPWVLRLSAQHIQPMGDSPLLPGWSELFVAGIERNFALGNQDLMILL
                     QGVSSKKAETGSLSTLEALLERALMLGLGWRPTETVTFLSVFYQEQRKFSFFFHNELK
                     LNHNERWSSQLAVDIFEGKADSALGTYNKNDQFTYQLSWAF"
     gene            complement(2431..3636)
                     /locus_tag="VIG33_14540"
     CDS             complement(2431..3636)
                     /locus_tag="VIG33_14540"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEY8272106.1"
                     /translation="MQVGRKIGKYQSMTRQQEISIYKNNILIGTLARTSKGAVFKYAK
                     AIEASENLCFKMPVSQIEYVIEGHNLHPFFANLLPEGLRLKLLLERNKISKDDLFNIF
                     RLAGTDFIGDIHTEFKTQDDSKIILKNKTIVNFYDYFLETLSVPEALTGIQEKISASM
                     ISLPLRGTNRKKSYIIKLNPKDKPHLIEDEFICLLYAKKCGLISSKAKIIEDRDHNKA
                     LMVERFDRISSDSKIIRVHQEDACQLLDIYPFDKYNTSINDITKELRKVLTAPQVGIL
                     NLMQMISFSYLIGNGDLHGKNISVTVSPETKVVSLTPAYDLIPTIIYGDTQMALPLDG
                     KKDNLKRKTFIEFGKRFGLRTEAIENMLNNLLLKATRNFDLFNQAPWSKKEQNLFKNL
                     FSSRLKDLR"
     gene            complement(3970..5442)
                     /locus_tag="VIG33_14545"
     CDS             complement(3970..5442)
                     /locus_tag="VIG33_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272107.1"
                     /translation="MINISVFFLSLLISFSAHGLTIEGFLHIPWDAVQQQLSLPQESP
                     TFKETFALKDLTVSSSFGPIAIPEVRLAVAYSLDPLSLQENPVSEQMFLTSKQIEISL
                     LIPAMTIQKQIHRSLNGVQVIVNLQASCSPIQLTQNQSAAKALVQWEARNQEAQGVIS
                     NFEFQWPPHSWVISSIQCTGPSGFADLIQQEIQNQLSEATQFENQIRDFLQTSLNQKV
                     NTLLAKALQPHLLQFQSSTYQYELKALQNISKSGIDFQVLVSNGKNESGNPITDLRGR
                     PLPNLSTTRPSLVFGEKGFQQFMQAALLQQTLQTQLNKLSAFSRLLHSRFLQFFIWPD
                     LMNFSKSAAFTLNTRVLPGSTLSLNAKKSWNMNLYLGSWLVAERENAPWNYLLIQNQL
                     QGQLALTIEKGQLMLAPSLFPSSNQLHSKITFGEDYVKRFDPCTIISKSLIEESVKKS
                     LFEKSHFLQIPKVQLEGLRPFLLQQFRFGSQNEMIFEFGP"
     gene            5622..>7257
                     /locus_tag="VIG33_14550"
     CDS             5622..>7257
                     /locus_tag="VIG33_14550"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY8272108.1"
                     /translation="MNTKYSFSEFFPRAHPLLLESKRVRSNFQLEETSQFLLVLRSER
                     KSWLEHGQTKAGLKQLKALTEELREIQGVQSAYSMATLEGAVENQGEILVGPVLDSIR
                     SKDRVAYVNNSPLLLGQLISKDLKSVLIVIEPAQLSPQELSALRRQLLSVAKKKLPGV
                     QVEMGGGPEVQRQFTEKLSEELILLLSLSLLCFCLVFLILIRGWRSLVLTFTTLVASN
                     LLILSLIAHFQIPFSILLSTLPIILSVAVISVVLHTLHRWAEVRHEKAFDFATPLVVR
                     VAGAWQVGREMLLPNFLGSLTTSLGFVALCSADIPLIRQYGWVVALSVLISWMIAQIF
                     LFIFMPFVDSDLRPWAHKKSLWALPMLKKSGPIVVAILAVCLVIGSQGSNINFSGRLF
                     DDLAKTDSARRITEKIDRQFGGIISYDLELTHRVKGAWKEPRQLAKLQGTLLKIRKLE
                     GVGSALAVTDLLESKSLESDKSRGSRPSDSGSLGSKAKLAETFFLYSMAEQNPIRNYL
                     NDNTESLRIAIRFHDLVGDKTQALQDKIKYLLKKEFP"
BASE COUNT         1992 a         1358 c         1702 g         2205 t
ORIGIN      
        1 ggaaatattc cggtgatcgc cgctggggga atttacactc atcaagatat tttaaaatac
       61 atcgacatgg gctgtgctgg agtgcagatg ggaactcgct tcctggcgac ccatgagtcc
      121 ggcgcaaatg cagaatataa aaaaatgtta gtggagtgta aagaagagga catcgagctt
      181 gcgagcagac caggatcacc ttgcggaatg cttttccacg tgatcaaaca gtcacctttt
      241 tatcagcaag ccgtggctcg tgaacgtgcc cctaagtgtg ataagggtta tctgttaaat
      301 aaaggtcatt gtccttcaaa gtacgaaaac gaaaaaactt tttgtatttg taatggtctt
      361 ttgtcttcga ttaatcttaa caataaagac gaaaaagatc tttatacagt aggcgccaat
      421 gcttatcgca ttgaccgcat tttgagcgtg catgagttga tggcggagct tcaagatatt
      481 cctttgagtg aagcaagttc aggagcgagc tgcctcaagc aagaaacatg tgttcatcaa
      541 aagatcattt agttggctct tgactcgtta ggcttctatg gatcagtcaa aaaattatat
      601 aactccgtca ggcgttgcaa aactcaaggc ggagtatcat gaattgatgc acgtcgaacg
      661 tcccaaggtt gtggaagttg tcacttgggc cgctggcaat ggggatcgct ctgaaaatgc
      721 cgattatcag tatggtaaaa agcgtcttcg cgagattgat aagcgcgttc gttttctgat
      781 ccagagaatt gataaagcag aagtgattga tccaaagacc atagattccg ccaccgtgtt
      841 ttttggagcc accgtgaaga tcatgaatga ggaggaacaa gtgatgactt accagattgt
      901 tggtgaggac gagtttgaca ttaaacttgg aaaaatctct tggaaatcac ccctggctcg
      961 ggctctgtta ggaaagaagg tcggcgatga agcggtggtt cataagccga atggcgaaga
     1021 gtacatcacc atcgaagaaa tccagtataa atagatggtt cattttaaga gtctcacccc
     1081 ctttttattt tttcttttaa tttcacagac agggtcattc gctaagtcag aaactcaagg
     1141 gaaagtcagt ttcctaggga tcagccgtcc tgaactcaag tctgactttg ccgagtctgc
     1201 ggatttcgtg gtggagcttg aaggagaaca taaatattat tttaataaaa atttgagctt
     1261 tcaggtctca ccttgggtgc gctccaattg ggcctcgaaa gattccgtac aaaagttttt
     1321 tctcgaggct caagaaatcg cgatcagcta taagaagtct tctcaaaaag tgatgattgg
     1381 ttttcatcga cagatttggg aagggacaga tctggtcaat cctatgtcct tgcagaatcc
     1441 tcagttttat cttgatccgc tagtgacgaa ggctcgaagt tcggctggcc ttttctattc
     1501 agatcaaatc ggaagcttca gctgggattt ttcttatatt cctaaacaag ggaaatcggt
     1561 tttaccgggg gaacacagcg cttggtggcc gcgaagtctg aatctgcctt tgcagaccga
     1621 gagcatggaa gtcaggttgc ccgaagatat cgcttatcaa attaataatg acgaagttct
     1681 ggatcgggct ttggaccaca atgtggggtt caggctgatg ataaatttaa attccttgga
     1741 tgtcgcaatg gcgggatttg agggaagttc tcaggctccg cttttaattc cagtgctgac
     1801 catgaatcct atcgaagtgt ctcctcgtca agtttttcag gtgacctcac cgatcgctat
     1861 tacgccgact tactttcgac gtcgagcgat ggccggagtg atgacttgga gcttgaatcc
     1921 ttgggttttg cgtttgtcgg cacagcatat tcaaccgatg ggggattcgc cgctgttgcc
     1981 gggatggtca gagttatttg ttgctggtat tgaaagaaat tttgcgctgg gaaaccagga
     2041 tctgatgata ttactgcaag gagtctcctc gaagaaagcg gagacgggca gtctgtcgac
     2101 tttggaagct ctgcttgagc gtgccttgat gttagggttg gggtggcggc ccacggaaac
     2161 cgtgacgttc ctgagtgtat tctatcaaga gcaaagaaag ttctcattct tttttcacaa
     2221 tgaattgaag ttgaaccata atgaacggtg gtcctcacag ctggcagtgg atatttttga
     2281 aggcaaagct gattcggccc tcgggacgta taataagaat gatcaattca cttatcagtt
     2341 aagttgggca ttttagaaaa acggcctgat tattttccga cctatttcct agctcggtta
     2401 ctgctccgct tgtgcctccg ataacctttg ttatcgcaga tctttcaatc ttgaggagaa
     2461 caggtttttg aagagattct gttctttctt ggaccatgga gcttgattaa ataaatcaaa
     2521 gtttctcgtg gcttttaaaa gaagattgtt taacatgttt tcgatcgctt cggttcgaag
     2581 cccaaacctt ttcccaaatt caataaatgt ttttcttttg agattatctt ttttgccgtc
     2641 taaaggtaaa gccatctgag tgtccccata tattattgtc ggaatcaaat cataggcagg
     2701 ggtcaaagag acaacttttg tctctggcga cacagttacg ctgatgtttt taccgtggag
     2761 gtctccattt ccgattaaat atgaaaatga aatcatctgc attaagttta aaattcccac
     2821 ttgaggagct gttaagactt ttcgcagttc ctttgtgatg tcattgattg aagtgttgta
     2881 tttatcaaaa ggataaatgt ctaataattg gcaagcatcc tcttggtgaa ctctgataat
     2941 tttactatct gaggaaatgc gatcaaatct ctctaccatc aatgccttat tgtgatcacg
     3001 atcttctatt atttttgcct tggaacttat gaggccgcac ttttttgcat ataataggca
     3061 gataaattca tcttcaataa ggtgaggttt atcctttggg tttaatttta taatgtaaga
     3121 cttctttcta tttgtgcctc ttaaaggcaa tgaaatcatt gatgctgaaa tcttctcttg
     3181 aattccagtc aaggcctcag gaaccgatag agtttccaag aaatagtcgt aaaaattcac
     3241 aatagtttta ttttttagaa tgattttaga gtcatcctga gtcttgaatt cagtgtgaat
     3301 gtcgccgata aaatcagttc cagcgaggcg aaaaatatta aatagatcgt ccttcgaaat
     3361 tttattcctt tcaaggagta atttaagtct tagtccctcc ggtaaaagat ttgcaaaaaa
     3421 aggatgtaaa ttatgtccct caataacata ttcaatctgg gaaacaggca ttttgaaaca
     3481 gagattctca ctagcctcga tagcttttgc gtatttaaag acggcaccct tgctggttct
     3541 tgccagggtg ccaatgagga tgttattttt gtatattgaa atttcttgtt gtcgtgtcat
     3601 ggattgatat ttaccgattt ttcgccccac ttgcattcca gtttaattcc cagcacatgt
     3661 aggacctgga gaacgtggga ccaacggact gagggctttc cactttctaa ttggctgata
     3721 aagttgaagg aagtgccgga aagagcagct aattgttttt gggttagatt ttgggtcttt
     3781 cgaacttcat gaataaagtt acctaagttt tgttcattgt tttttttcat atgatcgaat
     3841 gattttatat gaattaaata atttgtaaag ataaatcgca aaaaaataac acgatcatgt
     3901 gaatatttta ttgtttttcc tgttttttta gttaaattga gataaatcac acgatcgtat
     3961 gtgtacagct caaggtccaa attcaaaaat catttcgttt tgtgagccaa agcgaaattg
     4021 ttgcagcaag aaaggtctga gaccttccag ttgcacctta ggaatttgta aaaagtgact
     4081 tttttcaaaa agactttttt tgacgctctc ttcaatcagt gactttgaaa tgatggtgca
     4141 gggatcaaag cgctttacat agtcttcgcc aaaggtgatt ttgctatgca actggtttga
     4201 ggaagggaac agggaaggag caagcatcaa ctgacctttt tcgatcgtca gcgctaactg
     4261 gccctgcaat tgattttgaa tcaagagata attccaaggg gcattttcac gctcagcgac
     4321 caaccaagac cccaaataaa gattcatatt ccaagatttt ttagcattaa gactgagggt
     4381 cgatcctggc aagacccgcg tgttgagagt aaaagcggcg ctcttggaga aattcattaa
     4441 gtcaggccag ataaaaaatt gaagaaagcg tgaatggagg agccgtgaaa aagcagaaag
     4501 cttgttgagt tgagtttgca aggtttgttg tagtagagcc gcttgcataa attgctggaa
     4561 acctttttcg ccaaaaacga gagagggacg agtcgtcgaa agattcggaa gagggcggcc
     4621 cctcaggtca gtgataggat tgccactctc attttttcca tttgaaacca gaacttgaaa
     4681 gtcgatccca ctcttggaaa tattctgcag cgccttcaat tcgtattgat acgttgaact
     4741 ttgaaattgc aaaaggtggg gttgtaaggc tttggccagc aaggtgttga ccttttgatt
     4801 taatgaagtt tgtaaaaaat ctctaatctg attttcgaac tgtgtggcct cgcttaattg
     4861 attttgaatc tcttgttgga tgagatccgc aaaaccgcta ggccccgtgc attgaataga
     4921 cgaaatcacc caggaatgag gcggccactg aaattcaaag ttcgaaatca ctccctgtgc
     4981 ttcctggttg cgagcttccc attggaccag ggctttagct gcggattgat tttgcgttaa
     5041 ttgaatgggg ctacaactcg cctgaagatt aacaataacc tggactccat taagactgcg
     5101 atggatttgt ttttgaatgg tcatggcggg gatgagcaaa gaaatttcga tttgctttga
     5161 agttaaaaac atttgttcgg acactgggtt ttcctgaagc gaaagaggat ccaggctata
     5221 agccactgca agacgaactt ctgggatagc tataggtcca aatgaacttg aaactgtcaa
     5281 atccttgagc gcaaaagtct ctttaaaagt gggggattct tgagggaggg ataattgctg
     5341 ctggacggca tcccatggaa tatgaagaaa tccttcaata gtgagaccat gtgcggaaaa
     5401 agagatcaag agtgaaagaa aaaaaaccga tatatttatc atagtagtga tgtatcggac
     5461 ggtggtcgag tctagataag ggaaatatac acttgaagct atttcttaaa aatatttttt
     5521 ctaaattttt gagatcggaa cgaagtgtaa aactttttgc gatttttttt agtgttttag
     5581 ttctgatgtt gtgttttgtc tcgactcgag aaatcaagaa aatgaatact aaatattcat
     5641 tttcagagtt tttcccgcga gctcatcctc ttttgcttga aagcaaaaga gttcgttcta
     5701 atttccaact cgaagaaacc tcccagtttc tgttggtttt gagatctgaa cgaaaaagct
     5761 ggcttgaaca cgggcaaacg aaagcgggtt tgaaacaatt aaaggccttg accgaagagt
     5821 tgcgagagat ccaaggagtg caatccgcct actctatggc gacgctggaa ggggccgttg
     5881 aaaatcaagg agagatcctg gttggacctg tacttgatag catccgttcg aaagatagag
     5941 tggcttacgt caataatagt cctttattgc ttgggcagtt gatttctaaa gatctaaagt
     6001 cggtactgat tgttattgag ccagctcaat tgagtccgca ggagctgagt gcgcttcgac
     6061 gtcagctatt gagtgtggca aaaaagaaat tgccaggagt tcaagtagaa atgggcggtg
     6121 gcccagaagt gcaaaggcaa ttcactgaaa aattaagtga ggaattgatt ttattgcttt
     6181 cattgagttt gctttgtttc tgtttggtct tcctaatttt aattcgtggc tggcgctcac
     6241 ttgttttgac gttcaccacc ttagtagcat caaatttact tattctttcc ctgattgctc
     6301 attttcagat tccgttttcg attttgctct ccaccttgcc tatcatctta tcagtggcgg
     6361 tgatttcagt ggtgttgcat acacttcatc gctgggcgga ggttcgtcat gagaaagcct
     6421 ttgattttgc aacccctctt gtggttcgtg tcgctggggc ttggcaggtg ggcagggaaa
     6481 tgctgttacc caattttctc gggagtctta cgacttcctt gggttttgtt gctctctgca
     6541 gtgcggatat ccctttgatt cggcagtatg gctgggttgt ggctttgtcc gtgttaatat
     6601 catggatgat tgcgcaaata tttttgttca tctttatgcc ttttgtcgat tcggatttaa
     6661 ggccttgggc gcataaaaaa tctttatggg cactccccat gctgaaaaaa agtggaccga
     6721 ttgtcgtggc catcttggcc gtttgtttgg tgattggaag tcagggaagt aatattaatt
     6781 tttcgggccg cctctttgat gatttggcaa agacagactc ggcgcgaagg atcacggaaa
     6841 aaattgatcg tcaatttggc ggtatcattt cctatgacct tgaactcact catcgggtca
     6901 agggagcctg gaaagagcct cgacaattgg caaaattgca gggaacgctt cttaagattc
     6961 ggaaacttga gggcgttggg agtgctctag cggtgacaga tctgttggag tcaaagtctc
     7021 tggagtctga taagtcgaga ggttcgcgac cttcggattc cggctcctta ggttcaaaag
     7081 caaagctagc ggaaacgttc tttttgtact cgatggctga gcaaaatccg atcagaaatt
     7141 atctcaatga caacactgaa agccttcgca tcgccatccg ctttcacgac ttggtaggag
     7201 ataaaactca agcgctgcaa gataagatta aatacttatt gaaaaaagaa tttccac
//
LOCUS       DATBTE010000388         3523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_667411, whole genome shotgun sequence.
ACCESSION   DATBTE010000388 DATBTE010000000
VERSION     DATBTE010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667411"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VIG33_14555"
     CDS             <1..589
                     /locus_tag="VIG33_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272109.1"
                     /translation="QQGISIILFAFALKNVGLLSIHRVPPPPISIQEVQSYLEERTLS
                     YNPLRQLQAQVAAENKVLIYSVLDEGNWLYYPFPHIGDWFTVNSYANILTQDPAALRA
                     FFSSRKISHVLYNGYRTNLIKDFPGDLGTYSHCFKLIIEGKPHDKVKIYELDQENSNC
                     YDKSTPEHESISLLKKNYPVFIDSPFEKKSRPSVN"
     gene            662..1405
                     /locus_tag="VIG33_14560"
     CDS             662..1405
                     /locus_tag="VIG33_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8272110.1"
                     /translation="MKVALSLLVYNEGESIEQTILRIYNDLKILNDDFELWIMDNHST
                     DKTLEILERLKLQLPQLRHFRQSENVGYAMNTLSSFKEPKADIYVTMDGDGQFTAKDL
                     AKMIDKLKEEAADIVVGWRVKRNDPLDRIIMNRGFTWVTQTLLGCKLHDLNCGYRVMT
                     KKAADLISVKFKEKFVGPEIYARACQHNLRMVETPVDHFPRIAGESVYTGSKISSVLK
                     MISYLWTLRTEIKSGIKKNAFLSHENGRI"
     gene            1386..2885
                     /locus_tag="VIG33_14565"
     CDS             1386..2885
                     /locus_tag="VIG33_14565"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEY8272111.1"
                     /translation="MKMAASKRYLSLLVFTLAGLYTYFMTRHVVSMHNQYMQMGDFAA
                     YDQVVWSLSELFSFKSSICGSIMNAWTTIGAKSAEAVPPLNLNYNALGIHFIPSLFFT
                     NAIANFIYNSPYTTYLMQTLAFSISSVLLFYVLVGQKVSPWIATALAISFLIHPANIG
                     ANVNAFHPVTMAIPFLIILYYFAITSKIKSYWLLFFFIGFIQENLWITLTAFTLIFLF
                     QKKWKRAAANLAIGLLFLILTTQFFIPYFNPEHTCPYCSIYGSPLGGSMAEIIKNIIL
                     KPGTAWSLLIRSEVMEWLNQLFKPTLYLVLLNPIAFLIAIAGIVPNILSTNKAMHIMW
                     GQYNALAMPFLYLGAGLGFNLLQSKMRFKVLRYSWLWAFVILWVSIGAAKDWAVSSTT
                     QMENIFTFNQPVYVNPDMKKSYDEVKERIPVDASVSATELFLNTINRRRLAFLFPVGF
                     LEADYVVIEKSNLTIPKENYQPRMNDVRSSGKYDLAVDDAHIQLWIKKK"
     gene            complement(2888..3514)
                     /locus_tag="VIG33_14570"
     CDS             complement(2888..3514)
                     /locus_tag="VIG33_14570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY8272112.1"
                     /translation="MDFNKEKDFTVDKGNVDKSNLDFLVEKFKTWKFSDLIWLQWESL
                     LLWLFNFFPALPGFVFRNFIGFCLFKEKKGFSWIQTNVIFVHTNRLKVGSHFGVNSGT
                     YINAVGTIEIGDYVLIGSNVTISSGKHPIDGVLPPIYARPSIPSKIIIEDDVWIGAGA
                     VIMPGVNLKKGTVVGANSVVTKSTEPYSIVVGAPARIISYRDKRNPKN"
BASE COUNT         1040 a          765 c          639 g         1079 t
ORIGIN      
        1 acaacagggt atcagtatta ttctctttgc atttgctctt aaaaatgttg gactgctctc
       61 gattcatcga gtgcctcccc cgcccatttc cattcaagaa gtgcaatctt acctggaaga
      121 aagaaccctt agctacaatc ctctccgaca attgcaagcc caggtagcgg ctgaaaacaa
      181 ggttcttatc tattccgttc ttgatgaggg aaattggctg tactaccctt ttcctcatat
      241 tggagactgg tttacagtca atagttacgc caacatttta actcaggacc cagcggccct
      301 gcgtgccttt ttttcatcgc gaaaaatcag ccatgttctc tataatggat atagaaccaa
      361 tctcattaaa gatttccctg gggacctggg aacttatagt cactgtttta aattaattat
      421 cgagggaaaa ccccacgaca aggtgaagat atatgaactt gatcaggaaa attccaactg
      481 ttacgataaa agcaccccag aacatgaatc catctctctc ctaaagaaga actaccccgt
      541 cttcatagat tctcctttcg aaaagaaatc cagaccttct gtcaactaag gggcgtatct
      601 atttatttta attgataaga gttctttttt tatgataccg aatagagagc agggagagaa
      661 aatgaaagta gctttatcac ttttggttta caacgaagga gaatccatag agcaaaccat
      721 tctccgtata tacaatgacc tcaaaatact taatgatgat tttgagttgt ggatcatgga
      781 caatcactcc acggacaaaa ccttagagat cttagagcgc ctcaaactgc aactcccgca
      841 attacgtcat ttcagacaat cagaaaacgt gggctatgca atgaacaccc tctcttcctt
      901 taaagagcct aaagctgaca tctatgtcac tatggatggc gatggccaat tcacggccaa
      961 agatttagcg aaaatgatcg acaagttaaa agaagaagct gcggacattg ttgttggctg
     1021 gcgagtaaag agaaacgacc ctctggatcg tattattatg aatcgtggtt tcacttgggt
     1081 cacccagact ttattaggtt gtaagctcca cgatctaaac tgcggctatc gtgtcatgac
     1141 aaaaaaagct gctgatttaa tttcggttaa attcaaagag aagttcgtgg gcccagaaat
     1201 ttatgctcga gcatgccaac ataatttgcg tatggtggaa accccggttg atcactttcc
     1261 acggatcgcg ggggaaagtg tgtatacggg aagtaaaata tcgtcagtgc ttaaaatgat
     1321 aagttatctc tggactttac gaacggaaat aaaatcaggg attaaaaaaa atgctttttt
     1381 gagccatgaa aatggccgca tctaagcgct acttaagtct ccttgtattc actttagctg
     1441 ggctttacac ctattttatg acccggcacg tagtttccat gcataatcag tacatgcaaa
     1501 tgggagactt tgctgcctat gatcaggtag tttggtcact ttctgaacta ttctccttta
     1561 aaagctccat ttgcggctca ataatgaatg cctggactac aattggagcc aaatctgcgg
     1621 aagcagtccc gcccctgaat cttaattata atgcgttggg aattcatttt attccaagcc
     1681 tgttttttac aaacgccatt gcaaatttta tttataattc accctacacg acttatttaa
     1741 tgcaaaccct agcattctcc ataagttcag ttcttctttt ttatgttcta gttgggcaaa
     1801 aagtctcgcc gtggatagcc accgctctcg ctatttcttt tctcattcat cctgcaaaca
     1861 ttggtgcgaa tgtaaatgca ttccatcctg ttactatggc aattcccttt cttatcattc
     1921 tctattattt tgccattaca tccaagatca agtcctattg gctgttgttc tttttcatag
     1981 gttttataca agaaaatctt tggattacct taaccgcttt taccttgatc tttttgtttc
     2041 aaaagaaatg gaaaagagcg gctgccaatc tcgccattgg ccttttattc ttaatactga
     2101 ccactcaatt ttttattcct tattttaatc cggaacatac ctgtccctac tgcagtattt
     2161 acggctcccc attgggtggc agtatggccg aaataatcaa aaatattatt ttaaaacctg
     2221 gcacagcctg gtctttactt atccgcagtg aagtgatgga atggctcaac caattattta
     2281 aacccaccct ctatctcgtg ctattgaatc caattgcttt tctaattgca attgccggca
     2341 ttgttcctaa tattttatct actaataaag ccatgcatat tatgtgggga caatacaatg
     2401 ccttggccat gccttttctt tacttaggtg cgggactggg attcaacctt cttcagtcaa
     2461 aaatgcgatt taaagtgctt cgatacagtt ggctttgggc cttcgttatt ctctgggttt
     2521 ctataggcgc agcaaaagac tgggccgtct catcaacgac acaaatggaa aatattttta
     2581 cctttaacca gccagtttat gtcaacccag acatgaaaaa atcctacgat gaggttaaag
     2641 aaagaattcc ggttgacgct agtgtttctg caacggagtt gttcttaaac acgattaata
     2701 gacgccgttt agcttttctt ttccccgttg gatttttaga ggcagattat gtagtcatcg
     2761 aaaaatcaaa tctgacaatc cctaaagaaa actatcagcc tcgaatgaat gatgttaggt
     2821 cttctggaaa atatgactta gcagtagacg atgcacatat tcagctttgg ataaaaaaga
     2881 aataaagcta attttttggg tttcttttgt ctctgtaaga aattattcgg gccggggccc
     2941 caacaacaat ggagtacggc tcagtacttt ttgtgacaac ggaattagcc cccacgaccg
     3001 ttcctttttt tagattaacg ccaggcatta tcactgcccc agctccaatc cagacatcat
     3061 cttcaattat aattttagac ggaatggacg gtcttgcgta aatcggtggg agaaccccgt
     3121 caatgggatg cttgcccgac gaaatcgtca cattacttcc aatcagaaca tagtcaccaa
     3181 tctcaatagt gcccacagca tttatatagg tccctgagtt aacaccaaaa tgcgaaccca
     3241 cctttaatct gtttgtatga acgaaaatca cattggtttg aatccaggag aaaccttttt
     3301 tttctttaaa gagacaaaat cctataaaat ttctaaatac aaatcctggt aaggctggaa
     3361 agaaattgaa aagccacaat aacaagctct cccattgcaa ccatatcaaa tcagaaaatt
     3421 tccaggtttt gaatttctca accaaaaagt ccaagttgct tttatctacg ttacctttat
     3481 ctacagtgaa atctttttct ttattaaaat ccacgttatc aca
//
LOCUS       DATBTE010000389        50952 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1828719, whole genome shotgun sequence.
ACCESSION   DATBTE010000389 DATBTE010000000
VERSION     DATBTE010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 50952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50952
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828719"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1422
                     /gene="miaB"
                     /locus_tag="VIG33_14575"
     CDS             85..1422
                     /gene="miaB"
                     /locus_tag="VIG33_14575"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY8272113.1"
                     /translation="MEQKNPDAGHGRGVFISTYGCQMNVNDTERMYSLLEMANYTPVE
                     TAEEASVIIINACSIREKPVHKVYSEVGTYRKMKEKNPELRIGVGGCVGQQEKENLIK
                     REPLIDFVFGTDTIDLLPNLVAQTYEGYKTKAVNAKVDHRAPYHVETLVRNPGVSTFV
                     NIAKGCDNFCTFCVVPYTRGREKSRPLAHVLNDVRSLIKRGAKEVTLLGQNVNSYHSE
                     CGADFADLLQRVARETDIERVRYTTSHPKDFNQKLADVMFANQPKVCEYVHLPMQSGN
                     SRILNKMNRGYTREEYLGKIKMIKQAIPNVVLSTDIIVGFPGETEEEFLDTMSLMQEV
                     AFETIFAFSYSPRPFTKAAKFEDQVPEDVKNERLNRLFDAHKKMAFELVKRYEGLTLN
                     VLVEKAESTEGKVSGRTPENKLVHFLGSNDLIGKTVPVKIAKAFPAVLRGELV"
     gene            1447..2010
                     /locus_tag="VIG33_14580"
     CDS             1447..2010
                     /locus_tag="VIG33_14580"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HEY8272114.1"
                     /translation="MKSSEVSSEKVDSHIVFSAEMPEGAPFFEKDLIRLKPYGLSVTT
                     DFARPFLLLKDDSHNYTLPVAINPLEAGVTLTQANKSVAPTTMHRFTETLLESLDMKI
                     EKCIFVEIKGHFQFVRLFLKNHPTLETLKMRADEVMSLCLHLDTPIYASKEYIAKSRV
                     MNAEMDGMSADIKAHPSIIQKSHPYIM"
     gene            2030..2554
                     /locus_tag="VIG33_14585"
     CDS             2030..2554
                     /locus_tag="VIG33_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY8272115.1"
                     /translation="MKTTISVRGFTPQIASDVFVADNARIMGDVKIGLESSVWYNVTI
                     RGDVMPIRIGNEVNIQDGSVLHGTYGKFGCTVHDRVTIGHLVMLHGCIIGRESLIGMG
                     SIIMDGAVIGEQSIVGAGSLVTENSNFPPRSLIIGRPAKAKRTLTEEEVKALSYSADN
                     YLLYKTWYELEEKR"
     gene            2619..4085
                     /gene="guaB"
                     /locus_tag="VIG33_14590"
     CDS             2619..4085
                     /gene="guaB"
                     /locus_tag="VIG33_14590"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY8272116.1"
                     /translation="MDQKIPYALTYDDILLVPQYSEIIPSEITPKSEFARGKFLNTPI
                     LSAAMDTVTENRVARIMAQMGGLGFIHKNMSVEAQALEVEKVKKYESGMIQDPITLGP
                     DQMVHEALEIMAKYSISGVPITINHMLVGILTNRDLRFETNVNQPIKNLMTHKEKLVT
                     AEVGTTLEQAKQILQKHRIEKLPVVDKNFTLKGLITIKDIEKAQAFPQATKDGHGRLF
                     VGAALGVGADSRDRADALVAAKVDVLCVDTAHGHSRNVVEMVKYIAKKHKEVIIVSGN
                     VVTADGTLALMEAGADVVKVGVGPGSICTTRVVAGVGMPQISAIVECAKAAAQKGKTI
                     IADGGIKYSGDITKALALGANSVMIGNLLAGAEESPGETVLYQGRTYKVYRGMGSLGA
                     MAKGSKDRYGQYDVTEDDKLVPEGIEGKVPYKGSATGIIHQLVGGIKSGMGYIGARNV
                     QELQLKAKFVQISAQGLRESHVHDVSITKEAPNYRMEN"
     gene            4036..5613
                     /gene="guaA"
                     /locus_tag="VIG33_14595"
     CDS             4036..5613
                     /gene="guaA"
                     /locus_tag="VIG33_14595"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY8272117.1"
                     /translation="MMSVSLKKLPTIEWKTKMTERGFVILDFGSQFTQLIARRLRELG
                     FFSEIHAFNFSIDEIKKRKPLGIILSGGPNSVYEPLSPRREVRELLEVAPVLGICYGM
                     QLLAHQLGGEVEKSDHREYGLNRVAWRETFGAVPADQQVWMSHGDVVKKAPPGFQIVG
                     VSGSGHAAAMKGPRAWAIQFHPEVSHTFKGIEIIKAFAHSFCAGTPTWNAPHITEVLI
                     KDIQNKVAAHEHVLCALSGGVDSSVVAALLTKALGPERVHCVFVDNGLLRKNEFENVL
                     AAYKNIGLNVKGVDASKEFMAELKGKEDPEQKRKTIGRLFIEVFDKAYDHGTNIQFLA
                     QGTLYPDVIESVSSVGGSVTIKSHHNVGGLPEKMKLKLIEPVRELFKDEVRKIGAELG
                     LPPEMLSRHPFPGPGLAIRCLGEVTEEKLKILKECDDLFVSFLREKNIYSEIWQAFCV
                     LLPVKSVGVQGDSRTYDHVLALRAVTSSDGMTADWYPFDFAFLREISNRITNKVRGVN
                     RVVYDVTSKPPGTIEWE"
     gene            5742..6386
                     /locus_tag="VIG33_14600"
     CDS             5742..6386
                     /locus_tag="VIG33_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272118.1"
                     /translation="MKFKLRAALFVMSVLTLVSRPMMAHAESALETDDSFFKSVAPVE
                     DDDLDRSYKKNELVKSTETQKKIESHVKDEFSSTHQGIMPAYEAKMMDQKVVAENKRR
                     QAAIMSRLNLSAGAVLTCVSKTGQNFQGTHATVMWMIQPNGTVSETAIKSTDIENIEI
                     QKCIQEVASHLDFSAAKTDLLNKSLVEYTYKFKLKKKTSLGSHKKKIRRSTASQ"
     gene            6471..10010
                     /gene="dnaE"
                     /locus_tag="VIG33_14605"
     CDS             6471..10010
                     /gene="dnaE"
                     /locus_tag="VIG33_14605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY8272119.1"
                     /translation="MSFVHLHIHSEYSLLEAACRIKAISKKAAEYGMPAVALTDNGNM
                     FAAAEFYFAAKDNNIKPILGFDAYIAPGSRLEKKQDRDQIAQFPRRLVFLAQNIEGYR
                     NLNKLSTIGYQEGFYWKPRIDYEVIEKYNAHLLCLTGGLRGEVADIFLNEGPDQALEK
                     IRKLHNIFGDRLYLEMCRTGLPEWQKLNPFLLEASKITGVPVVATNDVHYMSPDDQLA
                     QEVLICIGTNKTLADEGRFKLGSSEFYFKKPEQMQKLFEDVPEAVARTLEIADRCEVK
                     FKLKDDTGKPIYFLPTFPTENGVSLKDDIAAKALKGLKERFSEAALRNEPIAEEKKPE
                     YFSRLDFELNVIDRMGFNGYFLIVQDFINWAKNQGIPVGPGRGSGAGSLVAYSLRITD
                     LDPLPNFLLFERFLNPERISMPDFDIDFCQDRRQEVIQYVTQKYGQESVSQIITYGKL
                     QARAAIKDVGRVLGMTFPEVDVVTKLIPDKLGVTLKEALEMEPRMRELMDMNPTVNTL
                     VELAQRIEGMVRHAGIHAAGVIIADGQLVRHAPLYRGADGEQVVQYDMKHAEKMGLIK
                     FDFLGLKTLTHINHAIQLIEKNRGLKIISNLIPMNDPKVFEMMSRGDTAGIFQFEGEG
                     ITDATRKIRPSSFGDITAITSLYRPGPMANIPEFTDRKHGKSPVEYLIEDSKEVLSET
                     YGIMVYQEQVMGIASRIAGYSLGEADMLRRAMGKKIKEEMDRQRIRFMDGAKAKGYEE
                     KASNDLFDLMYKFADYGFNKSHAAAYSVITMQTAWLKCYYPTEFFAALLSTELSDTDK
                     IVKYSKDAAKHGLLVKPPHVNFSEYLFGAHGDEVYFGIGAIKGVGESAVTAIVEARES
                     LPEKKFANLDEFFNTIDTRRVNKKVIECLIRAGAFEGLGAHRAQLIAGYQKYLDRAEG
                     KRKDRELGQTSLFDLGPSDEIDVKLENTKPWTRSALLSYEKEVLGFYLSDHPLKGYEN
                     LAEIWITCKVAELPQFAKQEAAQVQTAVQKTKENRWGRDANKKKVVVAGLISDLRELI
                     TKKGTRMAFAKIEDLSGSCELVIFPDAFARNEMMCRDEKPVLITGSLEADEGSAKIMV
                     DSVSPMEDILKKTKHMVLHLERIPANDYERLQGLLKDHPGSTHVSFEIDLKEVNRRVQ
                     LEVEETIAVSISNEFFESIHSVFGKTDFIELRT"
     gene            10053..11222
                     /locus_tag="VIG33_14610"
     CDS             10053..11222
                     /locus_tag="VIG33_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272120.1"
                     /translation="MKKIWMLFTGLMVYAQVVFAQSGDLIEKSFTGTSNEKTPQAAKR
                     DIQEQAAQKISEEFIKELIGEERYAKNKTLIQNKVEKISNRYIPFSKPSELMQEGAAY
                     KMTVVLKVSLKELKTILQEHSLLAENDTVPLALPLISFVDKVSLKTFRWWKQDEGSNK
                     TFLVSQNRQFENALRGAFQKYNFYLIKPLPLGLQIPRTYQNERLNLDDMQFLSQYFGA
                     PLMVEGQVQYSKSPESSNRYWIEVKLVALQVSNGRPIADVSRKFETEMGVFESSVDKK
                     IRETVDATAQELALQVYEAWQRGSLGTTVLRVTFRGKIPFNQQEAFKEKLKNQVHEIR
                     NIRERVITADSMVYEVDTNLNPKDFSLKLSGLEIDGRKWNPASLSNNEVTMQLQR"
     gene            11232..12170
                     /locus_tag="VIG33_14615"
     CDS             11232..12170
                     /locus_tag="VIG33_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272121.1"
                     /translation="MKNFSLIAFSLILNFVSVACQTIDRTTPVIRREVKDQSFAGKSD
                     NGQPRKRLMVLPFIDSSAQHPEYLRDKARATFLKELNKSGEVVAVDSRDMKSEVLKPE
                     EKGEYDLKEIAKQAQGQGFNAVLEGKIIDVRVARKADKVGLVRQMKSEFEAVVRVRVV
                     STRNGQEVFNTVKTVNLEEPTVRVVERVETDKFLQNNPELIESIIRDAFLDFVPQVLA
                     SFGKVSWEGRIAALNGDRIYLNVGRTSGIQVGDLLKVLEQGDDVYDPESGGHIGTVPG
                     RLKGTLEVISYFGNDGSIAVIHSGSGFKENDRVELY"
     gene            12263..12338
                     /locus_tag="VIG33_14620"
     tRNA            12263..12338
                     /locus_tag="VIG33_14620"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12296..12298,aa:Val,seq:gac)
     gene            12576..12821
                     /locus_tag="VIG33_14625"
     CDS             12576..12821
                     /locus_tag="VIG33_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272122.1"
                     /translation="MGYKKNNLNHKYKLNSRLIYDRLEIMQKDLAWLGSQVGVGYQCI
                     LNNCRNQRILSPQNFERMPGALGVSRDELLLPLGGGK"
     gene            12821..13384
                     /locus_tag="VIG33_14630"
     CDS             12821..13384
                     /locus_tag="VIG33_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272123.1"
                     /translation="MAKRFTNTDKWDDDWFLELPPLMKCVWEYLRDKCDGGTGFAKVS
                     FTRMSRDIKGKVTREAFDKHFSERVHWINEDRLWVIGYLFEQFKNLSSTNKAHINMAK
                     KVLAEVEKDAVQMNTKAQSAVVKLSTLLRPSIEGPLTLDDSLDKTGEGQQTLIGYRLK
                     DKGYRINKKKRDENFEVGAEVFEGTDV"
     gene            13381..13740
                     /locus_tag="VIG33_14635"
     CDS             13381..13740
                     /locus_tag="VIG33_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272124.1"
                     /translation="MTPAEFNPIYKRLADAYPTQFASQTKAIAIAQEVKGLDSVWFER
                     LVNKIIAANDPFVKLGDLINAERRRLTQEFTTSEQSADFERLRRNSTGMGYESELRKL
                     GANSLWDAVTKAKTKQA"
     gene            13757..14011
                     /locus_tag="VIG33_14640"
     CDS             13757..14011
                     /locus_tag="VIG33_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272125.1"
                     /translation="MDNILKANEGVSKAVKDSEQAKECMKLINNFGLQSLVILIAPHL
                     MKEIKATDPETYYGLKIALQNFEKMTKDMLLKLSKELETL"
     gene            14028..14306
                     /locus_tag="VIG33_14645"
     CDS             14028..14306
                     /locus_tag="VIG33_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272126.1"
                     /translation="MSTSIMTTTNNVTQELPELSRLAFDVNVSNKVIGQYARQGYWIV
                     CCCNEGETDESFLSRAYSAGATHVFSQDADIGIIIEKEEYEMLWVRYL"
     gene            14337..14585
                     /locus_tag="VIG33_14650"
     CDS             14337..14585
                     /locus_tag="VIG33_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272127.1"
                     /translation="MSEEITKRKVESEEAKLLVLETANKIDMVLREAFGPINEQQAWL
                     LCGRVINLMFSRFYLPAIVQTEKRLTTSTAKTEENSGI"
     gene            14706..14954
                     /locus_tag="VIG33_14655"
     CDS             14706..14954
                     /locus_tag="VIG33_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272128.1"
                     /translation="MRNQDVWIESRKVFERLSKDACIGFLHGALDDEVFEPKNDTWMQ
                     LSEKELRDLCCDCYILAAKQILQGRVFKSENQPAHKIH"
     gene            14968..15117
                     /locus_tag="VIG33_14660"
     CDS             14968..15117
                     /locus_tag="VIG33_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272129.1"
                     /translation="MDSNSEERRWQQAVIADLKGFQAFNRAQGVANLAILILLVCLII
                     LVVFK"
     gene            15219..15497
                     /locus_tag="VIG33_14665"
     CDS             15219..15497
                     /locus_tag="VIG33_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272130.1"
                     /translation="MKTKTITLERFISINEVTGETWDVYRHDKDYRLDAGWKLIPCKV
                     TFEVPLPEPSVSITPSRLEEIHDEWIGTVTNMKFSTYLKHKLFPDWEG"
     gene            15498..16034
                     /locus_tag="VIG33_14670"
     CDS             15498..16034
                     /locus_tag="VIG33_14670"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEY8272131.1"
                     /translation="MRIISEQELKDILDKHGKWLRNEEGGERANLSSANLSSANLSSA
                     DLSSANLRFVDLSFANLSSANLRFVDLSSANLSSANLRSADLSSANLRFADLSSADLR
                     SADLRSAVGFKFTPLQVVNTKYFITIFDDHVVWGCRKMTFEELNKFELKDCTSQWDES
                     EFKLNKKIITEMIRYYRK"
     gene            16064..16345
                     /locus_tag="VIG33_14675"
     CDS             16064..16345
                     /locus_tag="VIG33_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272132.1"
                     /translation="MKLNLDAKIEVSVGCVEYLTKEVENLRQENKMLRIENTAINRFL
                     TLIESITPKNQSIGYGEDRLWQAKREIENAVESVEKESMAKSERVAVAE"
     gene            16375..16596
                     /locus_tag="VIG33_14680"
     CDS             16375..16596
                     /locus_tag="VIG33_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272133.1"
                     /translation="MTQEQLDEAFGPFRETTPVTLPKYIAVRNKVKELAQLINDECPE
                     SREKATALTNLQAVKMWANAAIAIHTKPE"
     gene            16725..16871
                     /locus_tag="VIG33_14685"
     CDS             16725..16871
                     /locus_tag="VIG33_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272134.1"
                     /translation="MRNIILNILYYGFILSVIFFFSYIGFEADFYLKKQAVKEAIKEM
                     KSEK"
     gene            16868..17101
                     /locus_tag="VIG33_14690"
     CDS             16868..17101
                     /locus_tag="VIG33_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272135.1"
                     /translation="MSLQALIEKIRTDDFDGTLSAAKALEALCQAAEVMRRALDYYQN
                     SGNYSAKDLLAYKDMKARDAIITVEEICKGVEG"
     gene            17098..17283
                     /locus_tag="VIG33_14695"
     CDS             17098..17283
                     /locus_tag="VIG33_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272136.1"
                     /translation="MRRSTMIEYINDLVGSGLCSNAETILSFIEDLGMLPPKSTKYID
                     CNGEGHLVNEWDEEDSK"
     gene            17280..17720
                     /locus_tag="VIG33_14700"
     CDS             17280..17720
                     /locus_tag="VIG33_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272137.1"
                     /translation="MSEVKPELSPLDSDGPELSCPYIDGVIKRYPESKNTLEYVRSIN
                     SQLRYDANGYKEIYQSLKSDYDQLKKENEELKEMSKFGWQCYQDRCEGAYIEVSIVKK
                     LNEKLDLAIEALDKISVGSMPGGSYSRRIIAQETLAKLRGENAE"
     gene            17710..17955
                     /locus_tag="VIG33_14705"
     CDS             17710..17955
                     /locus_tag="VIG33_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272138.1"
                     /translation="MLSEADKELLKKFEHSCSDARIGDVADLLSIIDRQSKALAKCVE
                     QRNDLFDPGGECPPDQHIQALKDYNKELKSIMQGGGE"
     gene            17952..18167
                     /locus_tag="VIG33_14710"
     CDS             17952..18167
                     /locus_tag="VIG33_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272139.1"
                     /translation="MRNKHAVRNEDESIWVYFSGEVPRNGESVCFIGDKTEYEVYKVL
                     WFIESNDFGSQARVYIRPKESNDEIQN"
     gene            18183..18377
                     /locus_tag="VIG33_14715"
     CDS             18183..18377
                     /locus_tag="VIG33_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272140.1"
                     /translation="MKKNTVLLFSALAMSGIMAGDYPKRRYNYRTPKDNNKFKLTPEE
                     IELMEEMTPKEKKKFLKGRV"
     gene            18374..18934
                     /locus_tag="VIG33_14720"
     CDS             18374..18934
                     /locus_tag="VIG33_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272141.1"
                     /translation="MKSKFALASEYMKIQQGLNTSATRYDAFLAGFDSRDAEIAMLKM
                     KCESLQAKFKSQDDHLKAMACESFNDNFKLIELEQEKRNLLSTAETDLMRIAELRDEN
                     KYLKERLARYEDKLLGATMTLDEKWDELKPIVLAYLEDGWSKECAFKKAGVKPYGKMY
                     RLCYLEDEEFKAKVREILDCGPEGRT"
     gene            complement(18913..18989)
                     /locus_tag="VIG33_14725"
     tRNA            complement(18913..18989)
                     /locus_tag="VIG33_14725"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18953..18955),aa:Ile,seq:aat)
     gene            complement(19093..19272)
                     /locus_tag="VIG33_14730"
     CDS             complement(19093..19272)
                     /locus_tag="VIG33_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272142.1"
                     /translation="MKKVDFKLKSREKPAPINSHYSIPMTAELKAKLYKLPPERNKIL
                     RECAEMLVKENEDSV"
     gene            19404..19841
                     /locus_tag="VIG33_14735"
     CDS             19404..19841
                     /locus_tag="VIG33_14735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HEY8272143.1"
                     /translation="MTLTFNKFEVPWLITALQEYGVQEIAGVKNNPRVIEYAAETSLA
                     AKEDSVAWCASFVTWCLETCDYKSTKSARARSYLDYGIKLEKPIPGCIVVYDRGGGMG
                     HVHFYLYSKDGKEYGCGGNQQDGVNVSGYDKARVLGYRWPMKA"
     gene            20006..20368
                     /locus_tag="VIG33_14740"
     CDS             20006..20368
                     /locus_tag="VIG33_14740"
                     /inference="COORDINATES: protein motif:HMM:NF020354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEY8272144.1"
                     /translation="MTPEGRIELEIFNFLRGIGIFCFKHDSVGIFDPVKKIYRKSSNP
                     NRVAGVSDVLGIAPGGRFIAIEVKSEKGRLTDEQRKFIRMVQDNGGIAFVARSARGVA
                     LELSKYFPNDEKIRRYYQ"
     gene            20365..20616
                     /locus_tag="VIG33_14745"
     CDS             20365..20616
                     /locus_tag="VIG33_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272145.1"
                     /translation="MIEEKEVKTEFVLRIISFDLKGEVTGNHAHRLRPEHWKTDIDGL
                     APMLVGFTNEMKLQLKDEGFTMRQHDHGTVEYVLQESGS"
     gene            complement(20800..21111)
                     /locus_tag="VIG33_14750"
     CDS             complement(20800..21111)
                     /locus_tag="VIG33_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272146.1"
                     /translation="MGKWDSGIQTDDDALFAYGHHTDNPVVRRKNYGGYCNSFACVEK
                     IIGDRSSNPNYKYMVYNDVRVLKTTDKQYKSCPDCGHALFWDEISEEITPKKTRTKKL
                     R"
     gene            21196..21546
                     /locus_tag="VIG33_14755"
     CDS             21196..21546
                     /locus_tag="VIG33_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272147.1"
                     /translation="MALYNDKGTDELTGKLEGFIQFFREKTGKDIDREYLKKVLSEIY
                     DQKRRQLINQSFGTETPATLRLLIDAFDDLLPVVQEQILAIAEEPSEEVLQLPDLLPE
                     EEVIKPRKRKPKNG"
     gene            21539..21961
                     /locus_tag="VIG33_14760"
     CDS             21539..21961
                     /locus_tag="VIG33_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272148.1"
                     /translation="MARPVGTRAKITQAALKDMERLGVNPIEMLKAVYDRAITAFDGH
                     RGDTDKSDAGPSYLAVAQQAAANLAKYKHPALSAIAFKDMGESEEDRKPITTEEAMRI
                     IRSDPFAPDEIKEIPNEQVIDAMKSGIQAPLLPGGKNE"
     gene            21954..23327
                     /locus_tag="VIG33_14765"
     CDS             21954..23327
                     /locus_tag="VIG33_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272149.1"
                     /translation="MSDGLIDLAEAIQELNSRWKPHEAQIPIGRALFYQGAKDIFVCA
                     GRNFGKTELAGYCSWRYAAEHPGSENYIFEPYSKQAREILWASQRIQNFGPEQWVEGL
                     NTTEMRITFKNGSFIKLEGSDNSAAMAGIKPKGLIIYDEFKDHRFTSIDNFEPNRAAF
                     DVPALFIGTPPMVHNHYVDYMDLAKSDSNWRFFHAPTSSNPFIKKSWLERKKAEKIKM
                     GDLEGWLREYEALFVKGGKGSIFPQFFKMSRPNFDEVRPKDLNKWQILVSFDPASTSV
                     FGVVFLLFNEWTKQTIVFDELYVDDQALMTAREIHRQVEEKLQSYLKKVKDIRFGYDE
                     AAAWFRNEINEFSNWWLEPSSKSDFGVDGYINLVRQHMNKGLLTVCANCPKLIWELES
                     YQKDENGRIPKENDHNINALQYGLSCLGLRLDEDKPPIETDPDMQKRFHRWEEELEFS
                     STPAEID"
     gene            23350..23667
                     /locus_tag="VIG33_14770"
     CDS             23350..23667
                     /locus_tag="VIG33_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272150.1"
                     /translation="MDSEFKKRMQLLDPNYVAPDSERVKNRKPIDIKTEAPRKNEAGL
                     EYVPDTPVDSHLQQLYIDRMMDKDSVDKLDLIPEDPTEMMEENPNTKLKPDLAALNKK
                     RKK"
     gene            23667..23915
                     /locus_tag="VIG33_14775"
     CDS             23667..23915
                     /locus_tag="VIG33_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272151.1"
                     /translation="MELAAFIIAVIALIVSLASLTWLIAKQLSTHQVQLVPVDPFAQF
                     DPGKVGAKTSPMGDDFKELGEPLSPEEKEYFDKLKAKK"
     gene            23972..26086
                     /locus_tag="VIG33_14780"
     CDS             23972..26086
                     /locus_tag="VIG33_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272152.1"
                     /translation="MAQPQFFSDLDNPSSFSNKKPFFAIDLTKDAEVFTWLKDELAHL
                     QHDGTERLEKIKNNYMRYKGIQYLQQVYLPRDMPDTRKRYMPQVTVPLIRNVTDEIVS
                     RLLEFKPSVAVMPINDEEGDKVDAKIAKRFLSHVDYNDKLDQKILTWCRDSKIAGEAY
                     IWPNWNPDIGDVLNEDQIGATPVCIGDISNRNMSVFNTFYQKVRRFEDVDYVFYVTYE
                     YTEGLKRDYPDKESIIKSDNVRYYDTEKMTDETLEGFSYKFHFYYRPTKYLPTGFEAC
                     FTELGILKSGPFPFRHKKLPMIRLVDQQNPEELHGESSMEYTRGIAAQFNNMTNLIIK
                     QQMLCAHPKWFYEAASLDEQLLGNDTNIVKLKPGSKAPVLAQGAPVSQQVFDFREKLK
                     EEFYSMSKSNSVVQGEPPQGVTAFVALQFVSESESRRMSTDVQVLNAAIRDLYDMDLK
                     LAAQFYKPEDERTMMLLGPDGKWLTKSYNPKSLQGPFAVMVQNQTALPESRAVRTQFV
                     LDMSERYPGLLPKEQVIEMLGLGQSEKFMDLGSAAARAAEEEQEIILDQGQQIEPAEW
                     EDHITHWKIHTMKIQDIGFKTATSPESQEMMKDHILATEMLMIEQAKRSPQLTQLLLM
                     LPNFPIFYVDSQAQMAAGMLTGTIPAVMQGAQGAMQAMSPPMPEEPQPGAQGKPMNSS
                     PSDISQGVGSEPTPAGAKPTTA"
     gene            26103..27323
                     /locus_tag="VIG33_14785"
     CDS             26103..27323
                     /locus_tag="VIG33_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272153.1"
                     /translation="MAEAVITPMNNSPAATATAKAPDMAGDAPKTFDELQAAIDGKKA
                     EDRKAKKEAQEAREPKGETKDKPTDISRADSKGKEAKSKDKEPKESKDKEEDDSKKEE
                     PKPKKTLKYKKGEDEAEIDEDAMFTHLINGQEVQVSARDLLNNYSGKTAWDKKFSEVD
                     RKAKDFEGKYSKVQQQLKGLFEEQDPEMRIWKMAEVAGMNPIQFKKFYLDDNISLVEK
                     YYSMTDEERDSYFLQEENKYLRHKNETVAQAEKTRQAQAELSKKLEGLRQAHQVSEDD
                     FWSTYESIAAVADDGKLEQDGIKSAKDITPEKVIEIYQKEHLFSAGIEAMKSTSIKPE
                     LQGNALKELVDTAFAQGLKPQDIPDMISEIWGVKRRENKVAEKVKEREEFISGKKEVA
                     TPKTNREPMFFSDL"
     gene            27386..28603
                     /locus_tag="VIG33_14790"
     CDS             27386..28603
                     /locus_tag="VIG33_14790"
                     /inference="COORDINATES: protein motif:HMM:NF033394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEY8272154.1"
                     /translation="MAQMSLTASSALFKTKFGKMSENAYNSYNPMLGTIKKQYDFTGD
                     EMKVAVPTFFSGGVGSGSLPTANPASAVKATLTAKKVYATTEIDRESLKASSNDEGSF
                     VNGMKWVSEKTVEAYNRNASRILFSDGTGALGTTTAADFVDVTGTTFGVGDTAYALFT
                     AASWVEGYWEENDYVNVGTAADVLEVIAVDNTLRKVTFEKISGTTDFGTGGAGTSKII
                     YMQNSKDNDPMGLKGVCDATTSTLYGVDVARRWMATQIAAASAGISEDIMNELVITIQ
                     KKCGKAPKKLVTSYTQYRKISNFLEDKKVYQIEARAPELKGIVGWKALSYMSDAGEIA
                     IIPDRMCAEDRVYGVNDDYITSYHRPDFGWFDDDGTVFLRTSGDAYAARYGGYYQNYI
                     IPSFQGVVTGLAT"
     gene            28674..29006
                     /locus_tag="VIG33_14795"
     CDS             28674..29006
                     /locus_tag="VIG33_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272155.1"
                     /translation="MSSKQVLCPQREMRLMSVKVDGTGTAALSGTCSNNMTLTDNGTG
                     DYTLTYNLPFLRAPEIVATAITDNIYCKIGASAVGSCQILTENLSGAATDADFHVIII
                     GSDTADAQ"
     gene            29015..29752
                     /locus_tag="VIG33_14800"
     CDS             29015..29752
                     /locus_tag="VIG33_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272156.1"
                     /translation="MTAGYSVRKFPIKSPQFKPRLMTFGVTTGHAQVETLTFPAVAGA
                     TQGDFVVLYNEAGQSIAAYIDIDAAGTAPTSPLYTAATYKVAISVATGDLAAAVATAF
                     RTAVNGVLPDVTFTLASATCICTQDIVGDCSNAVLKNSDGTGVGSITNSVGTEGLAAV
                     MTTGGYDGTLTQTATGVYTITFERPFLRIPEYGITTVTDNRVPRISSGTVSTLVVEVS
                     NLSGGATIDGSFHVLVVGSDAKDAIRG"
     gene            29836..30921
                     /locus_tag="VIG33_14805"
     CDS             29836..30921
                     /locus_tag="VIG33_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272157.1"
                     /translation="MSVKGYPSNQKLIQSASGITHNYATVVPTDEYRNALDAPRFAFR
                     VNSATVPRTCGASTGNALSGPTVVVDTSTVAKVGDIFRAETGNAAFVEVPIVSVSTNG
                     FILASRLSTPPASGDTFYILRSVTQRTDENGSQVATIDTAGLATSAKQDAEAVLVGAV
                     NETAPASDTASSGLNGRLQRIAQRITSLIALLPASIGQKAMTASFPVVLASDQASVPV
                     AATLSAETTKVIGTVNLSAAQTLATVTTVSTVTAVTTLGTITNAVPTKAPVNANGSGG
                     GQTSLTATTASSEAAPANAVGFILQADPANTDPIRYRIGAAASTVNGIQLAQSQDTGF
                     IPCAATVSICATVSGTNKYAIQWILST"
     gene            30932..32119
                     /locus_tag="VIG33_14810"
     CDS             30932..32119
                     /locus_tag="VIG33_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272158.1"
                     /translation="MRIILLSLLISFQALAGLPPTTSKVSGDSTDLTTYKYQFPNFTG
                     THTGTTVSLGVNAIAGGGTNNGSLAVTNGGVVYTDGTKLMNSGAGTANQYLASIGAGA
                     PAWTSFVAPTIQKFTSTGTTTGYNFTITAGNATVGATYTNNGQTFTVLSTIAAATTLY
                     TTATGAPEASGTLTKATGTGDATLTFSAATATATYTLPTSPRNPVYIRVRMVGGGGGG
                     AGSADATNDGGNGGDGAASRFGAALIIANGGLGGISAASSGIGGTGGSASLGTAVGTS
                     LTGGKGQGFSGGATSQRQAGGGGAPSPFGGAGGGGAAATAGQNASANTGSGGAGGGAP
                     VAGFNGPGGGAGGFADALITSPSATYQYVVGANGTAGTAGTSGFVGGTGAAGYIEVTE
                     YYQ"
     gene            32222..32455
                     /locus_tag="VIG33_14815"
     CDS             32222..32455
                     /locus_tag="VIG33_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272159.1"
                     /translation="MKQITENTLIPISLVMTLLGGAAWLTAVWYDTKANAVSITEIKS
                     EQKILAKDLVEMQKKTIEDLATIKVKLNIKSQQ"
     gene            32483..32683
                     /locus_tag="VIG33_14820"
     CDS             32483..32683
                     /locus_tag="VIG33_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272160.1"
                     /translation="MLEGKEAEGSFGGFGGYSVDITDKGLLEIGVSVRIDLVAEAKKL
                     AEKTATPIDDTAIAWLEKLMSK"
     gene            32693..32926
                     /locus_tag="VIG33_14825"
     CDS             32693..32926
                     /locus_tag="VIG33_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272161.1"
                     /translation="MTWLINLLVPVIEWILNLGIKQLMAWWKERKDKEDVQKKKSAEL
                     EQSKKDLQKDLKGAGDDEKAQEDAIDNFFKRTR"
     gene            32871..33176
                     /locus_tag="VIG33_14830"
     CDS             32871..33176
                     /locus_tag="VIG33_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272162.1"
                     /translation="MMKKLKKTLLIISLSALASCGSSSLPDAPEIEQGAPIYGTDANG
                     VMFVKYFHLVNSKNHVETNLSVAQGRERILFITDLESYNRSELYVAELERLAKKRCE"
     gene            33173..33490
                     /locus_tag="VIG33_14835"
     CDS             33173..33490
                     /locus_tag="VIG33_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272163.1"
                     /translation="MITQKQKITGTVVDSEAVTFDMANLTAVEFQVTTTGTATSGTYK
                     IQKSNDGTHWVDIASATGAFTTATTLMTTCTGVVASQVRLLWGLASGTVTYVMYIQGK
                     ELV"
     gene            33490..33864
                     /locus_tag="VIG33_14840"
     CDS             33490..33864
                     /locus_tag="VIG33_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272164.1"
                     /translation="MGYMNSSFGIDTNRDYLYGDSQDPFDINKPQGQGSLGSEAMNKE
                     IATRFTPENAAVTGGAGFLAGGPAGAAVSVGGSLLTNYLAQKAATERQKRQQSVQIEN
                     EYTKNMGDALTQQLAAYRGALR"
     gene            33871..34110
                     /locus_tag="VIG33_14845"
     CDS             33871..34110
                     /locus_tag="VIG33_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272165.1"
                     /translation="MKQAIDMMKKAMAMMEQAQRRMNSAEDEESEEVETDYMPTEDSS
                     DSSFGNSNSGGEEDDLEERKQKKNMIIASMKRKQY"
     gene            34123..35028
                     /locus_tag="VIG33_14850"
     CDS             34123..35028
                     /locus_tag="VIG33_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272166.1"
                     /translation="MRRLETLIDNCREITGNNRYDSDSGRSQKVFVEYFNNAANSLAR
                     EIINAKSKFFVKEKIVTVVSGQSLYSWPNDIFLFNVDTMEWSTNGRDYFPLSKGIIKD
                     RFNVTSGTAFSYIPRNDGFLLNPPQTSGYVRINYIKQPSRLEKRSGKITARTMAGQVL
                     SALTIDSTEASFDPTYISRDNYISVVDKFGAQVAKRIVFTSVNSTTGVFTLSPITLDT
                     GETVSVGDYVCVGLDSCNLPPYPTTCEDYLMLYVEYQSKYGDASKWSAEIKNNLAAVA
                     GQIITSFGLMSDDITQIPITCTDFL"
     gene            35038..38040
                     /locus_tag="VIG33_14855"
     CDS             35038..38040
                     /locus_tag="VIG33_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272167.1"
                     /translation="MPQFTDKHYADFGGLDTTTNLLKMDPKTQRRGSKNFRYNNDDQL
                     TQRYGFQHITELSSAFKFGLAEYKYRDLNTGESKVEILGVGSDGFLHRLRNTRLKIVR
                     SSGSVVYYSVFYNGDDWTFALYGSAMDVIYSTHYDNTYTLSQLVTYVNLSAITGIALS
                     IVDENDSVVTCAIPAYLMNTVIVKEFSTAVTNTEYNALYYDELVQCPSSSALFPEVLD
                     SAITSLPDFEGLTSTNLNNCIYLTCGGFPIKYDGKSAYRAGVPAVLPQIGSNPLNASG
                     ISAAQATTTLEVVFTISPGSATFGATYTNNGVTFIVSATVVGATSILMLGAGGPTASG
                     TLTKASGAGDATLTFSAVDRGLTAGSYAYKFKFGHIDSNGAENLGDYGVSNYFGTNGY
                     IQSLPSGAGHNSITLTYYGFVNDSRFPVYACKVNGAQNIATAGGTINVDSGHNIKVGM
                     LVQIPISNAPTGFNGYSYIISKVTAVAATTITLASGRTSGIYPFVTGSTTLIVDNQIL
                     QAGYRETYFDNTITDIQTSARQWQPQTIVGSFLRVYRTKVGQTTGPFYKMRDLPLPLG
                     AMGSTSFTDIISDANLIEEFDDSEGGPLPRACRYIGQWQKQLVQGGRPYDTTLKDDYY
                     PSYYAAVNLVNNWSTNSTEDRFYINEETALCDFQSVYWADPLNAEAFPTSGLNEEDFQ
                     GKHADQIKGFGENKEALFVMKDRTAGMLTGTLATNDIQKEILEIDAGCSSHNSIQEVN
                     GSLVWLDGVAGFWACVAGRLPEYFGWKIQDDFRKNNQKANSQKLLPGRAKSVVNRIDS
                     QYICYIPAGYVSDVATGDPSSYSIMFVFDFSATEDGKRNCWYQWQDVSPNAGLLYTST
                     DELIFSENRAASYRLWKQKRTATKYDMADHNAAIEFNAKNAWLNYGTPTIDKDFVNSW
                     INSVEGGFTLTVQQYYNYMDTAASSYNVTMPTGTSKVSVKKSANLNVPKLSALSLGFY
                     HNTKNELVTIDGWEVVLAQDYDPGEPKI"
     gene            38040..38624
                     /locus_tag="VIG33_14860"
     CDS             38040..38624
                     /locus_tag="VIG33_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272168.1"
                     /translation="MSNTRIKSLAKLREYRAGMDMEEYLSTEFLQNLNFIDQKLRSLD
                     QAVVVNDICVRYTSASGQSIPSSVLTVLNFETKDFDSDSLVTVGTSWRFKAKEDAIYE
                     ASGFIVFTSGGGWGVGEEVAVNIHINGVSYARIGGVIMQAAHGTYVEIPLVACPFKLR
                     TNEYMDLRVYQSSGASLSLLGGINWISIKKIGAY"
     gene            38704..39603
                     /locus_tag="VIG33_14865"
     CDS             38704..39603
                     /locus_tag="VIG33_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272169.1"
                     /translation="MGTAYKYDQNGNPIPVGEVINGTYQGAWFDTPEQAKSPAVSNLD
                     NDYSKGYTRGKKEFYDDPDMQDLKKRNEDLAKGYDGNTLGAIRGEARDQIQGQRSNYM
                     RQLQSRVGKAGIGGARGAAMAGAADKGFMDSTASSERKMAIDNSNLQREGTGKLQDFI
                     FRQKYGTLGSAIGEQQAGLAERGKYAAAQANQSGGGGMSWLCTEIAKNENWTMSDLKA
                     LKKLLLFTEKHDKEASERYQKDAPTLIAIMKDKSFDFMRLQPTIKCIISLVKIGLIEM
                     AYENYKNMTMDLVNTYWGDYGRA"
     gene            39590..41509
                     /locus_tag="VIG33_14870"
     CDS             39590..41509
                     /locus_tag="VIG33_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272170.1"
                     /translation="MAERDYLAELLLNQRLPEINLADEAVQKVRNMKAAAAPPPVVAP
                     RPRPAAPVDDGGIDLKKIESQAIYQAALQAKQKAAVDKIRAMTAKPSPTPVPTPTPQV
                     TPTPEPQKSMVKMPDGNMAQRENEIEISADPLNEEDWIQPKSTDPKSLSANRDSYNVI
                     GYNLDKTIKQQDALVGRAEMARKPYVDRLEKMVDAYMQPVPKFQSSYDEYAKKMLEQE
                     ATEKRVARPDFQPPQRQEEEDILTKAIYAFGPGLGAMALGEDAALAAPTAQKQAYALR
                     ASDIAQKNKEYDQELAAYRANAVREKEKGVNSSLRGIALKLLAEGERDDFNKQQDTQD
                     KRMGEATKILSGLSADESKRLDAAIQDRQELLKANVDKGLISVKDYQDHVEKLAAFQN
                     QKDIADANRDAQNKRLDIEEAGRNQRAKESNATMLKATGMRGDAQANVAGIRAAAKGS
                     KTGGKPLTISAAESMSNANTALSSLEDINSIVTKNPEMFGKGQNILNQIAIATGMGDT
                     GTKAASIDAELGAIAQRIGVYLEGGKLAEGDIKRYRKMLPELGDKPEIVQNKIASLKR
                     LLEQKHNNDLESYRASGYDVSRFERKQISAGITEGVGKKNEPGKFPMTVRKGTQSTTV
                     ENIKELVEARKEGWK"
     gene            41509..43107
                     /locus_tag="VIG33_14875"
     CDS             41509..43107
                     /locus_tag="VIG33_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272171.1"
                     /translation="MGWRDRIQDEAPAPTKSTWKDRVQDEYKPDLSELMNYEDPSKKD
                     YPGTVRGAIKALPLAGTVAGGAFGTVAGGPLGPLSGPVGAGLGGAAGKSVENILDHYL
                     YGDEKSYNDILVDPAKEALNSSTMEMLGPVTGELGNLGVKGLKNVSSSMSKIPKRAME
                     VYAERGPEVAKISAKSENDLAEGADQIRQDIQKSVGSFKSQQNANISNSLDANGRQVV
                     DMSGTINALEENKAKLNPIINADKIGKIQNQIDLIKRVGMDTKDGKFIMLARDAYDVQ
                     KHLQDQAEYLAPGQLFKKKDFVDITFQRAASRARQAVGSAAPEISAANAELGKVRRME
                     GNINKNLIAPDKPYAGLISAGTGENQINRRNLQRIGDAVGADFVPEAENLAAAHHFND
                     AGFLPKVQNGASMAPLIAGTGGIGELVRGDVRTGLSSLALGAMGSPIAIKSAIDTARV
                     AKQVAPIGVGNAMQSIPQFLQHSLGQEPEIEGYKMGDTIKLDLATDAQNFENEIKNDP
                     KLPPTQKAKRLNLLRKHGRIYIGN"
     gene            complement(43092..43316)
                     /locus_tag="VIG33_14880"
     CDS             complement(43092..43316)
                     /locus_tag="VIG33_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272172.1"
                     /translation="MSKRFLTTKDLMDTYGKSKSEEKPFDSAAFDKFMYYLSAIAAFI
                     FFVYSAVKIVPRLFLMIKGAPAPVDDQFPI"
     gene            complement(43374..43907)
                     /locus_tag="VIG33_14885"
     CDS             complement(43374..43907)
                     /locus_tag="VIG33_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272173.1"
                     /translation="MKYTILALIFVLSACKNGGDNSAATADPIYGEWYYEKVGSTQYS
                     ATGFIVNIQSNGNIEFQDGYLESDGNSMKAVMRKSIGTFTREGDVFHIKYSYDTCDRI
                     KQEDLYISAMNGKLLIQNYDKSVKFSLSPISGNSTIKTASYLEDKNCNILSKIEAASK
                     RVPASVKSKSFLDRVVK"
     gene            complement(43958..44170)
                     /locus_tag="VIG33_14890"
     CDS             complement(43958..44170)
                     /locus_tag="VIG33_14890"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8272174.1"
                     /translation="MQSIQLVFSSNLKRLRQKRKLTQEELAEAVDCSPRYIQMLEAGK
                     SWPRLKVIQALSKCLRVKETDLFRVK"
     gene            complement(44377..44784)
                     /locus_tag="VIG33_14895"
     CDS             complement(44377..44784)
                     /locus_tag="VIG33_14895"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8272175.1"
                     /translation="MDIDKIVISKIFKLMDEKNISQRQLAKDAKINYQNLNRLIKGHR
                     SIVKSDILPDLAKALGVTTEFLKSVNMSDKTDLFATEKPQESKTELLGQIMVNLNALN
                     EDQLRSVLGFTETALRPSSGVLDNLANPVKLTK"
     gene            45222..45410
                     /locus_tag="VIG33_14900"
     CDS             45222..45410
                     /locus_tag="VIG33_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272176.1"
                     /translation="MSYPMVGCGKFDERASRIANLAYDYLNLAQTLKISHERRKHYYS
                     RYHKLLNARFDHIRGVYA"
     gene            45403..45657
                     /locus_tag="VIG33_14905"
     CDS             45403..45657
                     /locus_tag="VIG33_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272177.1"
                     /translation="MHKNYYRYTFEKFDKDGNPTIQCNYDITGKIGEIFVYSGEVDEM
                     VKVDLEVFERRHVSKFAELQTEVYLIVKDQDFISSVRYGT"
     gene            45666..46625
                     /locus_tag="VIG33_14910"
     CDS             45666..46625
                     /locus_tag="VIG33_14910"
                     /inference="COORDINATES: protein motif:HMM:NF015776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEY8272178.1"
                     /translation="MSNITLFEKTKSLIHAQAKKFLELAKSHKAVNFEQEASFALQAL
                     QANNFLASTAANNPNSLIDAIHNVASIGLSLNPALKHAYLVPRDGKVCLDFDYRGLAH
                     LSYESGAVKFVQAEIVYENDRFVLRGRGQEPIHERDPFSQDRGGVRGAYTIAKTVDGD
                     FLVETMTIAEIYAIRNNTQAYKAFLDKKTKSCPWVSHEGEMIKKTVIRRASKMWPKSN
                     AGRFEKAMQVQDEVEMIDVTPQSKEPEKPDESPKKLEEIRQLLTAIDRSEEKYLGHLE
                     RVHKRDLKTLEDLSMAEIEQALIELYQISSKANKKPENKNEVA"
     gene            46612..47328
                     /locus_tag="VIG33_14915"
     CDS             46612..47328
                     /locus_tag="VIG33_14915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HEY8272179.1"
                     /translation="MKSLKDVESQIKTFQDKFGTDIMRAEQGSMAWLQTRLGVITASE
                     VSAAVAKIGSETRNTYMCQLVAEVATGMVEEINSKYMDWGKLNEDAARSSYEFANDTV
                     IEQVNFIFKDDTFREGASIDGFVSGQKKLVEIKCPYNPTNYIKFIVADKIKAEYQWQY
                     QFQMRVTGAETADFVQYDPRMIVQPIKVLKVERDDEYQKKFDELIPAFLHDMDSMLKL
                     IGIEYGDQWKRIAEMTPKIA"
     gene            47513..47713
                     /locus_tag="VIG33_14920"
     CDS             47513..47713
                     /locus_tag="VIG33_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272180.1"
                     /translation="MTKLQTEVLKFHTDFEARYAELEAAYKDKSYDQTSSLAAGIVGF
                     TKARADWLKREVEIDIAFSEKE"
     gene            47956..48237
                     /locus_tag="VIG33_14925"
     CDS             47956..48237
                     /locus_tag="VIG33_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272181.1"
                     /translation="MKTIEEQQKEFKQCGDDFADAAFPCTLEFTYEANPHMKYVISSK
                     GLSKREYFAAQIMTGMMTTPDNIGHRKISAHLAVELADELIEALNKEKD"
     gene            48585..49685
                     /locus_tag="VIG33_14930"
     CDS             48585..49685
                     /locus_tag="VIG33_14930"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEY8272182.1"
                     /translation="MANSKLKKFKDKKGNEYPYLFIDEKTGEFYAVKRIHLAIKKKPL
                     GKEFTKARSQVLTAIQEMMHEKPKKVGSALIKDFYEIMLAEKKAMSIKESTLRRMDTV
                     WRNNIEPYWQYLTPEEVDQDQVTKFMTWHRRNKPGVQFSNTFKYLGNIFQVMVDRDHL
                     SPGKRPKLIIPKDEQAHHDKDKGRYITDKEITAVLEKADDVTYLMIRIAHICGNRKME
                     IGSLEKERVISSGKHYVIVFDTDDTKTGMAREVPLPKDLTEKIAAQIASSKSKFLFPM
                     HSDPERHVYPKVIDDGWDAAKAAAKIKGRMRFHDLRHTAARNFASANVNPIIACTILG
                     MSLATYQKVYCKLKASDLIIAVEQSAARLGAE"
     gene            50022..50189
                     /locus_tag="VIG33_14935"
     CDS             50022..50189
                     /locus_tag="VIG33_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272183.1"
                     /translation="MRMLLLVMLQSFVLTVFFTMISQADVRTFPTKNCRDNFFNLKKY
                     ERRSEAPKSFK"
     gene            50234..50815
                     /locus_tag="VIG33_14940"
     CDS             50234..50815
                     /locus_tag="VIG33_14940"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEY8272184.1"
                     /translation="MIDFYKMETPDGSKVHILLEELGMKYQLRSPEADYLKISPYGGV
                     SAIIDLEGEYSGQIPIFESEAILNYLAEKSHHFLGANEFEKAQVMSWLMFQASVVGPT
                     FANYEYAKENAIPLMLKHFEEESKRLLGMMDARLAEKPYLAGDFYSIADIATYPWIAE
                     TLKSRAEWYEATPYLLQWAELMGRRPAVQKAFQ"
BASE COUNT        15249 a        10036 c        12173 g        13494 t
ORIGIN      
        1 tgcattgatt ttctcctagc gatcctctat aactcaaaag ctttaatgac acaattgcta
       61 acttcaggaa gtaggttcat ccccatggag caaaaaaacc cagatgccgg tcacggacgc
      121 ggcgttttta tttctaccta cggttgtcaa atgaacgtga atgatacgga acgtatgtac
      181 agccttttag aaatggcaaa ctatactccc gtggaaaccg ctgaagaggc gtcggttatc
      241 attatcaacg cttgtagtat ccgcgaaaag cctgtccata aagtttattc cgaggtgggc
      301 acttatcgca aaatgaaaga aaaaaatcct gagttgcgaa ttggcgttgg tggctgcgtt
      361 ggtcagcaag aaaaagaaaa tctgatcaag cgagagccct tgattgattt tgtttttggc
      421 acggatacga ttgatctttt gccgaatctg gtggcgcaga cttatgaagg gtacaaaacg
      481 aaagcggtga atgccaaagt ggatcaccga gcgccttatc atgtggagac tctggtcaga
      541 aatccggggg tctccacttt cgtaaacatc gccaagggct gtgataactt ctgtactttc
      601 tgcgtggttc cttacactcg agggcgcgaa aagagtcgcc ccttggcgca cgtccttaat
      661 gacgtgcgga gtttgatcaa gcgaggggcc aaagaagtca ctttgctagg gcaaaatgtg
      721 aattcttatc atagtgaatg cggtgcggat tttgcggatt tgctccagag agtggctcgt
      781 gaaaccgata tcgagagagt ccgttacacc acatctcatc cgaaggattt caaccaaaag
      841 ctagcggatg ttatgtttgc taatcaaccg aaagtttgtg aatacgttca tctgcctatg
      901 cagagcggga atagccgaat tcttaataaa atgaatcgtg gatacactcg cgaggaatat
      961 ctaggaaaaa tcaaaatgat taagcaggcg attcccaatg tcgtgttatc aactgacatt
     1021 atcgttggat ttccgggaga gactgaagag gagtttcttg atacgatgtc tttaatgcag
     1081 gaggtggcgt ttgagactat tttcgctttt agttattctc cgcgtccgtt caccaaggcc
     1141 gcaaaatttg aagatcaagt tccggaagat gttaagaacg aacgattgaa ccgtcttttt
     1201 gatgcgcata aaaaaatggc ttttgagctt gtcaaacgtt acgaaggact cacattgaat
     1261 gtattggtcg aaaaagcaga atcgacggaa ggaaaagttt caggacgcac gccagaaaac
     1321 aagctcgtgc atttcctggg ctccaatgat cttattggca agacagtgcc ggtgaaaatc
     1381 gcaaaagctt tccctgcggt tcttagagga gaattggtat aatggatctc atgaatcggt
     1441 attataatga aaagctctga agtgagttct gaaaaagtgg attctcatat cgttttttct
     1501 gcggagatgc cagagggggc tccgtttttt gagaaagatc tgattcgttt aaaaccctac
     1561 ggcttgtcag tgactacgga cttcgctcgt ccatttttgc ttttaaaaga tgatagccat
     1621 aattacactt tgccggtggc gatcaatccc ctagaggcgg gagtgaccct cacgcaggcc
     1681 aataaatcag tggctcccac caccatgcat cgctttaccg aaactttatt ggaaagcctt
     1741 gatatgaaaa ttgaaaaatg catttttgtg gagatcaaag ggcattttca gtttgttcgt
     1801 ttgttcttaa aaaatcatcc gactcttgaa actttgaaaa tgcgtgcgga tgaagtgatg
     1861 tctttgtgtt tacatctgga cactccgatt tatgcttcaa aagaatatat cgcaaaaagt
     1921 cgggtcatga atgcggaaat ggacggaatg agtgcggata ttaaagctca tccttcgatt
     1981 attcaaaaga gccatcccta tatcatgtag tccgtcttga gggagattta tgaaaaccac
     2041 catttccgtt cgtgggttta cgccacaaat tgccagtgat gtttttgttg ctgataatgc
     2101 gcgcattatg ggggatgtga aaatcggttt ggagtcttca gtttggtaca atgtgacaat
     2161 tcgtggggat gtcatgccga ttcgcattgg aaatgaagtg aatatacagg atggatctgt
     2221 tttacatggg acctacggta aatttggctg tacagtccat gatcgtgtca ccatcggtca
     2281 tttagtcatg cttcatggct gcatcatcgg gcgagagtcc ttgataggga tgggaagcat
     2341 cattatggat ggcgccgtga tcggcgaaca aagcattgtg ggggcgggct ctctggtaac
     2401 ggaaaattcg aattttccac cgcgcagctt gattatcggc cgcccagcaa aggcaaaacg
     2461 gacgcttacc gaggaagaag tcaaggcttt aagctactcg gcagacaact atcttctcta
     2521 taagacgtgg tatgaattag aagaaaagcg ttgatgtctt tttggtctcc atgacagaat
     2581 agccccattc aaatcaaaaa aatatgacgg gggctctcat ggatcaaaaa attccatatg
     2641 cattgacata cgatgatatc ctattggttc cgcagtattc tgaaattatc ccttcagaaa
     2701 tcactcccaa atcagaattt gcacggggga agtttctcaa tacaccgatt ttatcggcgg
     2761 ccatggatac ggtgactgaa aaccgcgtag cccgcatcat ggcgcaaatg ggtggccttg
     2821 gttttattca taaaaatatg agtgtagagg ctcaggctct ggaagtggaa aaagttaaaa
     2881 agtacgaaag cggcatgatt caagacccta ttactttagg acctgatcaa atggtgcatg
     2941 aagctctgga gatcatggct aagtattcca ttagcggggt gccaatcact atcaatcata
     3001 tgctggtggg cattttgacc aatcgcgatt tgcgttttga aaccaatgtg aatcaaccga
     3061 ttaaaaattt aatgactcat aaagaaaagt tggtcacggc ggaagtgggg accacgcttg
     3121 aacaagctaa gcaaatcctg caaaaacatc gtattgaaaa gcttccggtg gtggataaaa
     3181 actttactct gaaaggtttg atcactatca aagatattga aaaggcccag gcctttcctc
     3241 aggcaaccaa agacggccat ggccgccttt ttgtcggtgc ggccttgggg gtcggtgcag
     3301 attctcgcga tcgtgcggat gctctggtgg cggcaaaggt ggatgtgctt tgtgtggata
     3361 cggcccatgg tcactctcgc aatgttgtgg agatggtaaa gtatattgcg aaaaagcaca
     3421 aagaggtcat catcgtctct ggcaacgtgg tcactgccga tggaactttg gccttgatgg
     3481 aggcgggagc ggatgtcgtg aaggtcggcg tcggtcctgg aagtatttgc accactcgcg
     3541 ttgtggctgg tgtgggaatg ccgcagattt cggccattgt agagtgtgca aaggcggctg
     3601 cacaaaaagg gaaaacgatt attgctgatg gcggtattaa atattctggc gatatcacca
     3661 aggctttagc cttaggtgcg aactccgtca tgatcggtaa ccttttggcg ggggctgaag
     3721 agtctccggg agaaacggtc ttgtatcagg ggcggactta taaagtttat cgtggtatgg
     3781 gcagtcttgg ggcgatggcg aaaggttcta aagaccgcta tggacaatat gacgttaccg
     3841 aggacgacaa gttggttcct gagggaattg aagggaaagt tccttataaa ggctccgcaa
     3901 caggcattat ccatcaactg gtggggggca taaaatccgg gatgggttac ataggcgccc
     3961 gcaacgttca agagttgcaa ttaaaagcga agtttgtgca aatcagcgct cagggccttc
     4021 gggagtctca tgttcatgat gtcagtatca ctaaagaagc tcccaactat agaatggaaa
     4081 actaagatga ccgaacgtgg ttttgtaatt ttggattttg gctcacaatt cactcaattg
     4141 attgcccgcc gtttgcgtga gttgggcttt ttctctgaaa tccatgcttt taatttttct
     4201 attgatgaaa tcaaaaaacg taaacctttg gggattatcc tcagcggtgg tcccaattca
     4261 gtttatgaac ccctgtcgcc acgccgggag gttagagaac ttcttgaggt ggctccagtc
     4321 ctgggaattt gttatgggat gcaattgctg gctcatcagc tgggcggtga agtggaaaaa
     4381 agtgatcatc gtgagtatgg cctgaatcgt gtggcctggc gagaaacttt cggtgccgtc
     4441 cctgcagacc agcaggtctg gatgagccat ggggatgtgg tgaaaaaagc tccgccgggg
     4501 tttcaaatcg tgggagtttc tgggtcaggt catgcggccg cgatgaaggg acctcgcgct
     4561 tgggctatcc aatttcatcc agaagtcagt catactttta aaggtattga aattatcaaa
     4621 gcttttgcgc atagtttctg cgcaggaaca cctacgtgga atgctcctca tattactgaa
     4681 gttttgatta aagatattca aaacaaagta gcggctcatg agcatgtcct gtgcgccttg
     4741 agtggtgggg tggattcttc agtggtggcg gctttgttga cgaaagctct ggggcccgag
     4801 cgcgttcact gtgtttttgt tgataatggc ctcttgcgaa aaaatgaatt tgaaaatgta
     4861 ttggcggcct ataaaaacat cggtttgaat gtcaagggtg tggatgcttc aaaagaattc
     4921 atggcggaac taaagggaaa agaagatccc gaacagaaac gcaaaacgat tggccgactt
     4981 tttattgaag tttttgataa ggcctatgat cacggcacga atattcaatt tttagcgcaa
     5041 gggactcttt accctgatgt gattgaaagc gtgtcctccg tcggcggcag tgtcactatc
     5101 aaatcccacc acaatgtggg tggtttgccg gaaaaaatga aattgaaatt gatcgagccg
     5161 gtgcgtgaac tctttaaaga tgaggtgagg aagatcggcg ccgagctagg cctgcctcct
     5221 gaaatgttgt cacgtcaccc cttcccggga ccggggttgg cgattcgctg cttgggagaa
     5281 gtcaccgaag aaaagctcaa gattcttaaa gagtgtgatg atttatttgt ttcgttttta
     5341 cgggaaaaaa atatttactc ggaaatttgg caagccttct gcgtgctctt gccagtgaaa
     5401 tccgtggggg ttcaggggga ttccagaacc tatgatcacg tcctggcttt acgagccgtg
     5461 acttcttcgg atggtatgac ggcggattgg tatccctttg attttgcttt tctcagagaa
     5521 atctccaatc gaataaccaa taaagttcgc ggggtgaacc gtgtggtcta tgatgtcacg
     5581 agtaaacctc ctggcactat cgagtgggaa taaaagagcg cgaatgagac aagatagtgt
     5641 ctaataagta gacactatcg gcagatattt aaaacattta tttttctaaa cccatgtact
     5701 ccctcgctat aattgtcgat aaggacttta gcgggggtat tatgaagttc aagttgcgag
     5761 cagctctttt tgtcatgtct gtgctcacgt tggtgagcag gcctatgatg gctcatgctg
     5821 aatcagccct tgaaacggat gatagtttct ttaaatcagt agctccggtg gaagatgatg
     5881 acttagaccg tagttataaa aaaaatgaac tggtaaaatc cactgagact cagaaaaaaa
     5941 tcgagtccca tgtgaaggat gagttttcct caacacatca agggatcatg ccggcttacg
     6001 aagcaaagat gatggatcaa aaagtcgtcg ctgaaaataa aagacgtcaa gccgctatca
     6061 tgagtcgttt gaatctaagt gccggagctg ttcttacttg tgtctcgaaa acaggccaaa
     6121 attttcaagg aacccatgct acagtcatgt ggatgattca accgaacggc acagtctcgg
     6181 aaaccgctat taaatccact gatatcgaaa atattgagat tcaaaaatgt attcaggaag
     6241 tagcctccca tttggatttt agtgccgcga aaacagattt attaaataaa tcattggttg
     6301 agtatactta taagtttaag ctcaagaaaa agacgagcct tggctcccac aaaaagaaga
     6361 ttcgccgttc aacggccagt caatagaatg aattcgacgc ataaggttaa tttgagttct
     6421 gtactgcgga taaaattgaa cctcaaggta gtctaagtta tgaattattc atgtcttttg
     6481 ttcaccttca tattcactcc gaatattctt tgcttgaagc agcttgccga ataaaagcaa
     6541 tttctaaaaa agccgccgaa tacgggatgc cagccgtggc tctaaccgac aatggaaata
     6601 tgtttgcggc tgcggaattt tactttgcag ctaaagacaa taacataaaa ccgattttag
     6661 gttttgatgc ctatattgcc cccggctcgc gtttagaaaa gaaacaggac cgtgatcaaa
     6721 ttgctcaatt tccccgtcga ctggtctttc tggcgcaaaa catcgagggt tatcgtaatc
     6781 tcaataagct gtctaccatt ggctatcaag agggttttta ttggaagccg cggattgatt
     6841 acgaagttat agaaaagtac aatgcccatt tgctttgtct cactggtggc cttcgtggtg
     6901 aggtggccga cattttttta aacgaaggtc cggatcaggc tctggaaaaa attcgcaagt
     6961 tacataatat tttcggggat cgactttatt tagagatgtg ccgtaccggt ctgcctgagt
     7021 ggcaaaaact caatcctttc ttactggaag cttcaaagat tacgggcgta ccagttgtgg
     7081 ccacgaatga cgtgcactat atgtctcccg acgaccaatt ggctcaagag gttttaattt
     7141 gtatcggcac caataaaacg ctcgccgatg aagggcgttt caaattgggt tccagtgaat
     7201 tttacttcaa gaagcctgag cagatgcaaa agctttttga ggatgttccg gaagccgtgg
     7261 cgcgcacttt agaaatcgct gatcgctgcg aggtgaaatt taaacttaaa gatgatactg
     7321 gcaagccgat ttatttcttg ccaacctttc caactgagaa cggagtgtca ctgaaggatg
     7381 acattgctgc caaggccttg aaggggctta aagaacgttt ttcggaagca gctttacgta
     7441 atgaacccat cgccgaagaa aaaaaaccgg agtattttag tcgccttgat tttgaattga
     7501 atgtcatcga tcgcatgggt tttaacggtt attttctgat cgttcaagac tttatcaact
     7561 gggcgaagaa tcaagggatc ccggtgggac cgggacgggg gtccggagcg ggctctctgg
     7621 tcgcttatag tttgcggatc acggatctcg atccccttcc taactttttg ttatttgagc
     7681 gtttcttgaa tcctgagcgg atctccatgc ctgacttcga tatcgacttc tgtcaggatc
     7741 gccgtcagga agtgattcaa tatgtgactc aaaagtacgg tcaggagtcc gtttcccaga
     7801 tcatcactta tggaaaactt caggcgcggg ccgccatcaa agatgtgggc cgggttttgg
     7861 gaatgacttt tcctgaagtg gatgtggtga caaaattaat tcctgataag ttaggcgtga
     7921 ctctgaaaga ggctctggaa atggaacctc gcatgcgtga actgatggat atgaatccta
     7981 cagtgaacac cttggtggag ttagcacagc ggattgaagg catggttcgt catgcgggaa
     8041 ttcatgccgc cggcgttatc atcgccgatg gtcaattagt gagacacgcg cctctttatc
     8101 gcggcgctga cggagagcaa gttgttcagt atgatatgaa gcatgctgag aaaatgggtc
     8161 tcatcaaatt cgacttttta ggtttgaaaa cgctgactca tattaatcat gcgatccaac
     8221 tgatcgaaaa aaatcgtggt ctgaaaataa taagcaattt gattccaatg aatgatccca
     8281 aggtttttga gatgatgtcc cgtggggata cggcagggat ttttcagttt gagggtgagg
     8341 gcatcaccga tgccactcgt aaaatccgtc cctctagttt tggtgacatc accgcgatca
     8401 cttcacttta ccgcccgggt cctatggcca atatcccaga gtttacagac cgcaagcatg
     8461 ggaagtcgcc cgtagagtat ttgattgagg actcgaaaga agttttatcc gagacctatg
     8521 gaatcatggt ttatcaagag caggtgatgg ggatcgcttc tcgcattgca ggatactcct
     8581 taggggaagc ggatatgctt cgccgggcga tgggtaagaa aataaaagaa gaaatggatc
     8641 gccagcgtat tcgttttatg gatggagcaa aagccaaggg ctatgaagaa aaagcttcca
     8701 atgatctttt tgatttgatg tacaagtttg cggattatgg ttttaataag tcccatgccg
     8761 ccgcttattc ggttatcacc atgcaaacgg catggttgaa atgttattat cctacggagt
     8821 tcttcgcggc gctcttaagc accgagttgt cagatacgga taagatcgtt aagtactcca
     8881 aagatgcggc aaagcatggg cttttggtta aacctcctca tgtgaacttt tcagaatatt
     8941 tattcggagc ccatggcgat gaagtttatt ttggtattgg tgctattaaa ggcgtgggcg
     9001 aatcagcggt gacggccatc gtggaggctc gggagagttt gccggaaaag aaattcgcga
     9061 atttggatga gttctttaat acgatagaca cccgccgtgt gaacaaaaaa gttattgaat
     9121 gcttgatcag agccggtgcc tttgagggac tgggagctca tcgggctcaa ttgattgccg
     9181 gttatcaaaa atatttggat cgtgctgaag gaaagcgcaa agaccgagaa cttggccaga
     9241 cctctttatt tgatctcggg ccgagcgatg aaattgatgt aaaactagaa aacacgaaac
     9301 cttggactcg gtccgctctg ttgtcctacg agaaggaagt cttgggtttc tatctgagtg
     9361 atcatccctt aaaaggttat gagaacctgg cggaaatttg gatcacttgt aaagtcgcgg
     9421 agctcccgca gtttgcaaag caggaggccg ctcaagttca gacggccgtg caaaagacga
     9481 aagaaaatcg ttggggtcgg gatgcgaata aaaagaaagt cgttgtggct ggactgatct
     9541 cagaccttcg cgagctgatt actaaaaaag gcacgcgtat ggcctttgct aagattgaag
     9601 atctttcagg aagttgcgaa cttgtgatct ttcccgatgc ctttgcccgc aacgagatga
     9661 tgtgtcggga tgaaaagcca gttctcatca ctggatcttt agaagccgat gaaggatctg
     9721 ctaaaatcat ggtggactcg gtttcgccaa tggaagatat cttgaaaaag acaaagcata
     9781 tggtgttgca tctggaacgg attccagcca atgattatga acgtctgcag ggacttttga
     9841 aggatcatcc aggaagcact catgtgagtt tcgaaataga tctcaaagag gtcaatcgcc
     9901 gagtgcagtt agaagttgag gaaacaattg ccgtcagcat tagcaatgaa ttctttgaaa
     9961 gtattcattc tgtgttcgga aaaacggact ttattgagtt aagaacgtaa gctgaaaact
    10021 tataagctaa aaattgaaag aacaaggatt gtatgaagaa aatatggatg ctttttacgg
    10081 gactcatggt gtacgcccag gtcgtatttg cccaatcagg ggatctgatt gagaaaagtt
    10141 tcaccggaac ttccaatgaa aaaacacctc aagcggcaaa aagggacatt caagagcagg
    10201 cggcgcaaaa aatatccgag gagttcatta aggagctgat tggtgaagag cgctacgcaa
    10261 agaataaaac tttgattcaa aacaaggttg aaaaaatctc aaatcgttac atcccgtttt
    10321 ctaagcccag tgagctcatg caagaggggg cggcttataa gatgacggtg gttctgaaag
    10381 tgtcactaaa agaactcaaa actattctcc aggagcactc tttacttgcg gagaatgaca
    10441 cggttccttt ggctctgcct ttgattagct ttgtggataa agtgagtctt aagacttttc
    10501 ggtggtggaa acaggatgaa ggcagcaata agactttttt ggtttctcag aatcgtcagt
    10561 ttgaaaatgc gttgcgtggg gcatttcaaa agtataattt ttatttaatt aaacccctgc
    10621 cattgggatt gcagattccg agaacttatc aaaatgaacg tctcaatctg gatgatatgc
    10681 agttcttgag tcagtatttc ggtgctcctt tgatggtcga gggacaagtt caatattcaa
    10741 aaagtcccga atcttccaat cgctattgga ttgaagtgaa gcttgtggct ttacaagtca
    10801 gtaatggccg accgattgcg gatgtttctc gcaagtttga aactgagatg ggggtttttg
    10861 aatcttcggt ggataaaaaa atccgggaaa cagtggatgc cacagctcag gaattggcct
    10921 tgcaggttta tgaagcctgg caacgtgggt ctttagggac cacggtttta agagtcactt
    10981 ttcgtggaaa aattcctttc aatcaacaag aagctttcaa agagaagttg aaaaaccagg
    11041 tgcatgaaat tcgcaatatc cgggaacgcg tgatcacagc ggactccatg gtctacgagg
    11101 tggatacgaa tttaaatccc aaggacttct ccttaaaatt aagtggtttg gaaattgatg
    11161 gacgcaaatg gaatccagcg agcctttcta acaatgaagt gaccatgcaa ttgcaacgat
    11221 agggaaagta catgaaaaat ttttctctta tagccttttc tttgattcta aattttgtat
    11281 ctgtagcttg ccaaaccatt gatcgaacca ctccggtgat tcgacgtgaa gtgaaggatc
    11341 aatcttttgc cggaaagagt gataatggtc agccccgtaa gcgattgatg gtattgcctt
    11401 tcattgatag ctccgcccag catcctgagt atcttcgtga caaagctcgt gcgacttttc
    11461 ttaaagaact caataagtct ggtgaagtcg tagccgtgga tagtcgcgat atgaaatcag
    11521 aggtcttaaa gccagaggaa aaaggtgagt atgaccttaa agaaattgcc aagcaagccc
    11581 aggggcaagg tttcaatgcc gtgcttgagg gcaaaataat tgatgtccgc gtcgcacgga
    11641 aagcggataa agtcggcttg gttcggcaaa tgaagagtga gtttgaagcc gtggtccgcg
    11701 tccgtgtggt gagcactcgt aacggtcaag aagtttttaa cactgtaaaa actgtgaatc
    11761 ttgaagagcc tacggttcgc gttgttgagc gcgtggagac ggataaattt ctgcaaaata
    11821 atcccgagct gattgaatcc attatccgcg acgccttttt ggattttgtg ccgcaggttc
    11881 tcgcctcttt cggcaaagtc agttgggaag gacggattgc cgctctcaat ggcgatcgca
    11941 tttatttgaa cgtgggacgc acttccggca ttcaggtcgg ggatctgctg aaggttttag
    12001 agcaagggga cgatgtctat gaccctgaaa gtggagggca catcggaaca gtgcctggtc
    12061 gattgaaggg cacccttgag gtcattagct actttggaaa cgatggctcc attgccgtga
    12121 ttcattcagg ctctggtttc aaagaaaatg atcgtgtaga actctattga gagcggattg
    12181 atacgcaatg ttatcgacac gccttcgttg tctattcggg gatttttgag ttataaagaa
    12241 ttttctttgt gagttttcaa ttggtctgtt agcgcagttg gtagcgcgtt cccttgacat
    12301 gggagaggtc acaagttcga gtcttgtaca gaccaccaat tatattctac aagcctagta
    12361 atacacgcct taaatggttt gaaattacta ggcttttttc atttttcaga gccaaaaatc
    12421 agacttcgca tatttcgctt caacgcgaat atacacgtag agcgacgttt tgaatgtttc
    12481 ggatttgttt cgggagattt ttcccgattc ttcattaaac aaaaaaatac caattccaat
    12541 ttatcaatct gcacttccgc tggggggcgg gaaatatggg atataaaaaa aataacctga
    12601 atcataagta caaattaaac tctcggctga tctatgaccg cctagaaatc atgcaaaaag
    12661 atctggcatg gctcggctcg caagtcgggg tgggctacca gtgcatttta aataattgcc
    12721 gtaatcagcg cattctttcg ccacagaatt ttgagcgaat gcccggggct cttggtgttt
    12781 cacgcgatga gttactgctg cctttgggcg ggggtaagtg atggcgaaac gattcaccaa
    12841 tactgataag tgggatgacg actggttcct ggagttacca ccgctcatga aatgcgtctg
    12901 ggagtacctc cgcgacaagt gtgatggagg cactggcttt gccaaggtaa gcttcactcg
    12961 gatgagtaga gacattaagg ggaaggttac acgcgaagcc tttgataaac atttcagcga
    13021 aagagtccat tggataaacg aggataggct ttgggtaata ggctacctgt ttgagcagtt
    13081 caaaaatctt tcgtcaacca ataaagcaca tatcaacatg gccaagaagg ttcttgcaga
    13141 agttgaaaaa gatgcagtgc aaatgaacac caaggctcaa agtgcagtcg tcaaactttc
    13201 gaccctcctt agaccctcga tagagggtcc cctgaccctc gacgattctt tagataaaac
    13261 aggggagggt caacagaccc tcataggata taggttaaag gataaaggat ataggataaa
    13321 taaaaagaag agagatgaaa attttgaagt tggcgcggaa gtgtttgaag ggacagacgt
    13381 atgactcctg ccgaattcaa tcccatctac aaacgcctag cagacgcata tccgactcaa
    13441 ttcgcaagcc agaccaaagc catagctatt gctcaagaag tcaaagggct tgattctgtg
    13501 tggtttgaga ggcttgtgaa taagatcatc gcggcaaatg acccattcgt gaaactcggt
    13561 gatttgatta atgctgaaag gcgcaggttg actcaagaat ttaccacgag tgagcagtcg
    13621 gctgatttcg aaagattgcg tagaaactcg acaggtatgg gctacgaatc ggaattaaga
    13681 aaactcggcg caaattccct ttgggatgcc gtgactaaag cgaaaacaaa acaggcgtaa
    13741 agccaaagga gagaatgtgg ataatatttt aaaagcaaat gaaggcgtaa gcaaagcagt
    13801 caaagattca gaacaggcga aggagtgtat gaagttgata aataactttg gcttacaatc
    13861 actcgtaatc cttatagcgc cacatctgat gaaagaaatc aaagcgactg atcctgaaac
    13921 ttattacggt ttaaaaattg cacttcagaa tttcgaaaaa atgaccaagg acatgttgct
    13981 gaaactcagt aaagagttag aaacgcttta gcgaaaggac agggggcatg agcacttcga
    14041 taatgacgac caccaacaac gtcactcagg aattaccgga attaagcaga ttggcatttg
    14101 atgtaaatgt atccaacaaa gttataggcc aatacgcacg gcaaggctat tggatcgttt
    14161 gttgctgtaa tgagggcgag actgatgaat cttttttaag cagagcctat tcagcgggag
    14221 cgacacatgt cttttctcaa gacgctgaca tcggaataat cattgaaaaa gaagaatatg
    14281 aaatgttgtg ggtgagatat ctttaacctg acaaaacatg accaaggagc gagagagtga
    14341 gtgaagaaat aacaaagcgc aaagtcgaat ccgaagaggc aaaactttta gttcttgaga
    14401 ccgctaacaa gattgatatg gttttgcgag aagctttcgg accgataaat gaacaacaag
    14461 catggctctt atgcggtcga gttataaact taatgttttc tcgattttat ttgcctgcaa
    14521 tagttcaaac tgaaaaacgg ctaaccacat ccaccgcgaa aacggaagag aatagcggca
    14581 tataaaggat aaatatgaaa ataaaagata actttccaga ttttggcttt gaattaaaaa
    14641 cacaaattgt tgaaagcgaa actggtttca aatggcattg gttatttttt taaaggagcg
    14701 agagagtgag aaatcaagat gtatggattg aatcgagaaa agtatttgaa cgcctcagta
    14761 aagacgcatg tattggtttc ttgcatggcg ctctcgatga cgaagtgttt gagccaaaga
    14821 atgatacttg gatgcaatta tctgaaaaag aattaagaga cctttgttgt gattgctaca
    14881 tacttgcggc gaaacagata cttcagggcc gggttttcaa aagcgaaaat cagccagcac
    14941 ataagattca ttaaaggagt gagagatatg gattcaaatt cagaagaacg tcgctggcag
    15001 caagctgtca ttgctgatct taaggggttt caggctttta atagggcgca aggtgttgct
    15061 aatttagcaa ttttgatttt attggtctgc ttaattattt tagtggtgtt taaatgagcg
    15121 tagtaaaatt gattgatccc ccaaccacat ccccggcaaa aacggaagag aatagcgacg
    15181 cataacgcag ctaaggtagg aaaatttaga ggagaattat gaaaacgaaa acaatcacac
    15241 tcgagagatt catttccatt aacgaagtga ccggagagac ttgggatgtt tacaggcacg
    15301 acaaggacta tcgcttagat gcaggttgga aattgattcc ttgtaaagtc accttcgaag
    15361 tcccactgcc tgagccatcc gtgagcatta cgccgagtag actagaagaa attcatgatg
    15421 agtggattgg aaccgtgacc aatatgaaat ttagtacata tctcaaacac aagcttttcc
    15481 ctgattggga ggggtaggtg agaataatat cagaacaaga gttaaaagac atactcgaca
    15541 aacacggcaa gtggttaaga aatgaagagg gtggcgaaag agctaaccta agctctgcta
    15601 acctaagctc tgctaaccta agctctgctg acctaagctc tgctaaccta cgctttgttg
    15661 acctaagctt tgctaaccta agctctgcta acctacgctt tgttgaccta agctctgcta
    15721 acctaagctc tgctaaccta cgctctgctg acctaagctc tgctaaccta cgctttgctg
    15781 acctaagctc tgctgatcta cgctctgctg acctacgctc tgcggttggt tttaaattca
    15841 ctccattgca agtcgttaac actaaatatt ttatcactat cttcgacgat cacgttgtat
    15901 ggggttgtcg caagatgact ttcgaggaat tgaataagtt tgagctaaaa gattgcacat
    15961 cgcaatggga tgaatcggag tttaaactta acaagaaaat aatcactgaa atgattcggt
    16021 attatcggaa ataactaaag cgctaagcgc gaaggagaga agtgtgaagc taaatttaga
    16081 tgcaaaaata gaagtaagcg ttggatgcgt tgagtattta accaaagaag tagagaattt
    16141 acgccaagaa aacaaaatgc tcagaattga aaacacagcg atcaatcgct ttttaacact
    16201 gatagaatca attacgccta aaaaccaaag cattggttac ggtgaagata gactttggca
    16261 agcaaagcga gaaattgaaa acgctgtcga atcagttgaa aaagagagta tggcaaaatc
    16321 tgagagagtt gcagtagctg agtaaattaa ccgccgagag gcaaggagag aagtatgaca
    16381 caagaacaat tggacgaagc atttggacct ttcagagaga caactcctgt aacacttcca
    16441 aagtacattg ctgttagaaa taaagtgaaa gagctggctc agctaatcaa tgatgagtgt
    16501 ccagagagtc gagagaaagc aacggcattg acgaatcttc aagctgtgaa gatgtgggcc
    16561 aacgctgcaa tcgcaattca tacgaaacca gagtaattaa ttccttcggg gatgtagata
    16621 ggttcggggg ctaagactga acttgaaccc aagtgccctc ctgggcagaa atgtaccggg
    16681 aaagtgttca aggtgctgca ttcccgattt tttgaggtga gagaatgaga aatatcatat
    16741 taaatatttt gtattacgga tttattttat ctgtaatttt tttcttctca tacataggct
    16801 ttgaagctga tttttattta aaaaaacaag ctgtgaaaga agcaattaaa gaaatgaaga
    16861 gtgaaaaatg agcctgcaag cactgattga gaaaataaga accgatgatt ttgatggcac
    16921 actatccgca gcaaaagctt tagaagccct ctgccaagct gctgaagtga tgagaagagc
    16981 gttagactat taccaaaatt ccggaaatta ctctgccaaa gatttgttgg catacaaaga
    17041 catgaaggcc cgcgacgcta taattacagt cgaagaaatc tgcaaaggag ttgaggggtg
    17101 agacgtagca cgatgattga atatattaat gatcttgtcg gctctggttt atgctcaaat
    17161 gctgaaacaa ttctttcttt catagaagat cttgggatgt tgcctccaaa atccactaag
    17221 tatatcgatt gcaacggtga gggacatctt gttaatgagt gggatgagga ggactccaaa
    17281 tgagtgaggt gaagccggaa ttatctcccc ttgatagcga tggtccagaa ttatcatgtc
    17341 cgtacattga cggtgtaatt aaaagatatc ctgagtctaa aaatacactt gagtacgttc
    17401 gatcaattaa ctctcagcta agatatgatg ccaacggtta taaagagatt tatcaaagcc
    17461 tgaaatccga ttatgatcag ctcaaaaaag agaatgagga attgaaagag atgagtaaat
    17521 tcggatggca atgctatcag gaccgctgtg aaggagctta tattgaggtt tcaattgtaa
    17581 agaagcttaa tgagaaactc gatcttgcga ttgaggcatt ggataaaatt tcagttggtt
    17641 caatgcctgg tggttcatat agtcgccgta taattgccca agaaacttta gcaaaactcc
    17701 gaggcgaaaa tgctgagtga agctgataag gaattattaa agaaatttga acatagttgc
    17761 tctgatgcca gaattggaga tgttgctgat cttctatcta tcatagaccg ccaaagcaaa
    17821 gctcttgcaa agtgtgtgga gcaaaggaat gatttatttg atccaggcgg tgaatgccca
    17881 ccagatcaac acatacaagc gttgaaagac tacaataaag agctaaaatc aatcatgcaa
    17941 ggcggtggtg agtgagaaat aaacatgctg taagaaatga agatgaatca atatgggtat
    18001 atttctctgg tgaagttcca agaaatgggg aaagcgtttg ttttatcggc gataagactg
    18061 aatatgaagt atataaagtt ctttggttca ttgaatcaaa tgatttcgga tcacaagcga
    18121 gagtttatat tcgtccaaag gaatccaatg atgaaatcca aaactgatgc aagaggtggg
    18181 gagtgaagaa gaatacagtg cttctttttt ctgctctagc gatgtctggc attatggctg
    18241 gtgattatcc caagcgcaga tacaactata gaactccaaa agacaacaat aagtttaaac
    18301 tgacacctga agaaattgaa ctaatggaag agatgacacc gaaagagaaa aagaagtttt
    18361 tgaaaggtcg agtatgaaat ccaaatttgc gctggcttcg gaatacatga aaattcagca
    18421 aggtttaaat acaagcgcga cacgatacga tgccttcctc gcaggctttg actcacgtga
    18481 tgcggagatt gcaatgctga aaatgaagtg tgaatcgctt caggcgaaat tcaaatcgca
    18541 agacgaccac cttaaagcca tggcgtgtga aagttttaac gataacttta agttgattga
    18601 actcgaacaa gaaaaacgta acctcctctc aaccgcagaa accgatctta tgcgcatagc
    18661 tgagttaaga gatgaaaata agtatttgaa agagcgtctg gcgcggtatg aggataagct
    18721 ccttggggcg accatgacac tcgatgaaaa gtgggatgag cttaagccaa tcgtgcttgc
    18781 gtatcttgag gacggttgga gtaaagagtg cgcttttaag aaagccggtg taaagccgta
    18841 tgggaagatg tatcggcttt gctatttaga ggatgaggaa tttaaagcga aggtccggga
    18901 aattcttgat tgtggacccg agggcaggac ttgaacctgc gacctttagc ttattacact
    18961 aacgctctaa ccagctgagc taccaaggga tgaaatgtaa taaaccctgt attaactgcg
    19021 cccagggact gagggcttct catcaacttc taagctttcg ctccatcgac aatctcctcg
    19081 gcggctcaaa tatcaaacac tatcttcgtt ttctttcaca agcatttcag cacactcgcg
    19141 aagtatttta ttacgctctg gcggtaactt ataaagcttt gcctttaatt cggcagtcat
    19201 tggtattgaa taatgcgaat taataggagc gggtttttct cttgatttta gtttaaaatc
    19261 aacttttttc attccgatag catccaataa agttagaaaa attcaaagaa gaattttaga
    19321 aacataacgc acgctaattc agaattaatt cgaattcgat tagaataaaa tgccctgtca
    19381 ccattgagct tcgaggtgac gatatgactt taactttcaa taaattcgaa gttccatggc
    19441 tgatcacagc actccaagag tatggcgtgc aagaaattgc cggagtaaag aacaacccac
    19501 gtgtgatcga atacgcggct gaaacttcgc ttgctgccaa agaggactca gtagcttggt
    19561 gcgcatcttt cgtcacctgg tgccttgaaa cgtgtgatta caagtctaca aagagcgctc
    19621 gagcgcggtc ttacttggat tatggcatca aattagagaa accgattcca ggatgcattg
    19681 tcgtttatga tcgcggtggc ggcatggggc acgttcattt ttatttgtat tccaaagacg
    19741 gtaaagaata tggatgcggc ggaaaccaac aggacggcgt gaatgtctcg ggatatgata
    19801 aggcccgcgt actcggctat cgctggccga tgaaggcata gatgaccaca ctcctttatc
    19861 tctacctaac tggctggctc atcactggat catatcacgc aggtaaatgc gaagtagagc
    19921 caggtcactc gatgacaaag actgaatgca aggtattcat gagcgcggca agtgctggct
    19981 ggccttatta tttggtggtg ggtgagtgac tccagaaggg cgcattgagc ttgaaatatt
    20041 taattttttg cgtggtattg gcatattttg tttcaagcac gattctgttg ggatatttga
    20101 tccggtcaaa aaaatataca gaaaatcatc caatcctaat cgtgttgcag gtgtttctga
    20161 tgttcttggc attgctcctg ggggccgctt tattgcgata gaagtgaagt ctgagaaggg
    20221 ccgcctgact gatgaacaac ggaaatttat acgcatggtg caggataatg gcggcatcgc
    20281 ttttgtggcc aggtcggcgc gtggtgtggc actagagctt tcgaagtatt ttccaaatga
    20341 tgagaagatt aggagatatt atcagtgatc gaagaaaaag aagtgaaaac cgaatttgta
    20401 ttaagaataa tttcttttga tttaaaaggc gaagtgactg ggaaccatgc tcataggctg
    20461 cgtcctgaac attggaagac tgacattgat ggcctagctc ctatgcttgt gggcttcacc
    20521 aacgaaatga agcttcagct taaagatgag ggcttcacta tgagacaaca tgatcatggt
    20581 acggttgaat atgtgttgca agagagtgga tcttgaaatt cagagcattc agaacgcatg
    20641 ttgatgcaag tggttgggga tttccagaag aagtgataga aatgcgatgg gagcgcaacg
    20701 gaaagttctg taggtgcttt tgtgagcctg attgtaattg gcaaggcctt ttttatcagg
    20761 caaggattct caaaaagagc ctcaaagaat atattaaatc taccgaagtt tcttagttct
    20821 agtcttcttt ggagtgattt cttcgctgat ctcatcccaa aacagagcat gaccacaatc
    20881 tgggcaagac ttgtattgtt tgtcggtagt ctttaaaacc cgaacgtcat tgtaaaccat
    20941 atatttgtaa ttgggattgc tgcttcgatc tccgatgatt ttctcgacac atgcaaagct
    21001 gttacaatag ccaccgtaat tctttcttct aacgacagga ttgtcagtgt ggtggccgta
    21061 agcaaagaga gcatcatcat cggtttgaat tccagaatcc catttaccca tcaacttccc
    21121 ctttgataat cactatcgac aacggcgctc gctcttacaa tgatagtaat ataaaacaca
    21181 aattcaagga taactatggc gctttataat gataaaggga ccgatgaact cacaggaaaa
    21241 ctcgaggggt tcattcagtt ttttcgcgaa aaaacgggca aagacataga tcgggaatat
    21301 ttaaagaaag tattatccga gatctatgat cagaagaggc ggcaactcat caatcaatct
    21361 tttggtacag aaactccagc tactttgaga cttttgattg atgctttcga tgatcttttg
    21421 cctgtggttc aagagcagat cttggcaatt gccgaagagc catcagaaga ggttcttcag
    21481 cttcccgatc ttcttcctga agaagaagta atcaaaccaa ggaagcgcaa accgaagaat
    21541 ggctagacca gttggtacgc gtgcaaaaat aactcaagct gctttaaagg atatggagcg
    21601 acttggagtc aatccaatcg agatgctgaa ggcagtctat gaccgcgcta tcacagcttt
    21661 tgatgggcat aggggtgaca ctgataaatc cgatgcgggc cctagttatt tggccgtggc
    21721 tcaacaagct gctgcgaatc ttgccaagta caagcatccg gctctgtcag caatcgcttt
    21781 taaagacatg ggcgaaagcg aggaagacag aaaaccgatc acgacagaag aagcgatgag
    21841 aattattcgg tctgatccat ttgcgccgga cgagatcaaa gaaataccaa atgagcaggt
    21901 cattgatgcc atgaaatcgg gcattcaagc gcctttgttg cctgggggga agaatgagtg
    21961 atggcctcat tgaccttgcg gaagcaatcc aagagctgaa ttcaaggtgg aagccacatg
    22021 aggcgcaaat ccctattggc cgggctttgt tctatcaagg agcaaaagac atctttgtct
    22081 gcgccggccg taacttcggg aagactgaac tcgccggtta ttgctcctgg agatatgcgg
    22141 ctgagcatcc gggatctgag aattacatct ttgagccgta ttcaaagcaa gcgcgtgaga
    22201 tcctctgggc ttctcaaagg attcagaatt ttggtcctga gcagtgggtg gaaggtctta
    22261 acaccacaga aatgaggatt accttcaaga atggctcatt tattaagctt gaaggatctg
    22321 acaatagcgc tgcaatggcc ggaattaaac ccaagggact gataatttat gacgaattta
    22381 aagatcatcg tttcacttct attgataact tcgagcctaa ccgagcggct tttgatgttc
    22441 ctgcattatt catcggaaca cctccaatgg ttcataacca ctatgtggat tatatggatc
    22501 ttgctaagtc tgactcaaat tggcgctttt ttcatgctcc tacttcgtct aatccattca
    22561 ttaagaaatc ttggctggaa aggaagaaag ccgaaaagat aaagatgggg gaccttgagg
    22621 ggtggctccg ggaatatgaa gcactttttg ttaaaggtgg caaaggatca atctttccgc
    22681 agttctttaa gatgtcgcgt cctaatttcg atgaggttag gccaaaggac ctcaacaaat
    22741 ggcagatatt ggtctctttc gatccagctt ccacttcagt ctttggcgtg gttttcctgc
    22801 ttttcaacga gtggaccaag caaacaatcg tgtttgatga actttatgtc gatgaccaag
    22861 ctttgatgac agccagggag atccatcggc aagttgaaga gaagctccaa tcttacctca
    22921 aaaaagtgaa agacattcgc tttggttatg acgaagcggc agcctggttt cggaacgaaa
    22981 taaacgaatt ctcaaattgg tggctagagc caagttcaaa gtctgacttt ggtgtggatg
    23041 gctacataaa tttagtccgg cagcacatga ataaagggct ccttactgtt tgcgccaatt
    23101 gcccaaaact tatctgggaa cttgaaagct atcaaaaaga tgagaatggc cgaatcccaa
    23161 aagaaaatga tcacaacatc aatgcgcttc aatacggatt atcatgtctt ggtttaagac
    23221 ttgatgaaga taagccgcct attgaaaccg atcctgacat gcaaaaacgc ttccatagat
    23281 gggaagaaga actcgaattc tcttccactc cggcagagat tgattaacca ttacgattgg
    23341 agggctttaa tggattccga attcaaaaag cgtatgcagt tacttgatcc caactatgtt
    23401 gctccagact ccgaaagagt caaaaataga aaaccaatcg acatcaaaac agaggctccc
    23461 agaaagaatg aagcgggcct tgagtatgtt cctgatactc cagtcgatag ccaccttcag
    23521 caactttata tcgaccgcat gatggataaa gactcagtcg ataagcttga tttaatccct
    23581 gaagatccaa cggagatgat ggaagaaaat cccaatacga aacttaagcc agatctcgca
    23641 gcattaaata agaaaaggaa aaagtaatgg aactcgcagc attcattatc gcagtcattg
    23701 cgctgattgt gagcttagcc tcactgactt ggctgatcgc taagcagctc tcaacacacc
    23761 aggtgcagct tgtacccgtc gatccatttg cacaatttga tcctggcaaa gtaggcgcaa
    23821 agacaagtcc aatgggtgat gattttaaag agcttggtga accgctaagc cctgaagaga
    23881 aagagtattt cgataagtta aaagctaaaa agtaaaaaaa tataaataac acttattcaa
    23941 ttctcccata actagggagc ttggagaaaa cgtggcacag cctcaattct tttcggattt
    24001 agacaatccg tcgagctttt ctaacaaaaa gccttttttc gctattgatt taaccaaaga
    24061 cgctgaagtc ttcacttggc tgaaagatga gttagctcat ttacagcatg atgggaccga
    24121 gcgccttgag aagatcaaga acaattacat gcgctacaag ggtattcagt accttcagca
    24181 agtgtatctc ccaagggata tgcctgatac tcgtaagcgc tacatgcctc aagtaacagt
    24241 tcctttaatc agaaacgtga ctgatgagat tgtgtctcga ttgcttgaat ttaagccctc
    24301 tgtggccgtg atgcccatca acgatgaaga gggtgataag gttgatgcaa aaatcgctaa
    24361 gcgctttctt tcgcatgtgg attacaacga caaattggac caaaagatcc tcacttggtg
    24421 tcgtgattct aaaattgctg gtgaagctta tatttggcca aattggaatc ctgatattgg
    24481 tgacgtttta aacgaagatc aaatcggcgc gactccggtt tgtatcggcg acatttctaa
    24541 tagaaatatg tcggtattta ataccttcta tcaaaaggtc cgacgctttg aagatgtcga
    24601 ttatgtcttt tacgtgacct atgagtacac agaaggtcta aagcgcgact acccagataa
    24661 agaatccatc ataaaatctg acaacgttcg gtattacgat actgaaaaga tgaccgatga
    24721 aactctggaa gggttctctt ataaattcca cttctattat aggcctacaa aataccttcc
    24781 taccggcttt gaggcttgct tcacagagct tggaatcctc aaaagcgggc cattcccttt
    24841 tagacacaaa aaattaccaa tgattcgcct ggtggatcag caaaaccctg aagaactcca
    24901 tggtgagtct tcaatggaat atactcgcgg gatcgcagcg cagtttaata atatgacgaa
    24961 cttaattatt aagcagcaaa tgctctgtgc tcatccgaag tggttctatg aggcggcttc
    25021 tcttgatgag cagcttctag gcaatgacac caatatcgta aagctaaagc ctggttcaaa
    25081 ggctccagtc cttgctcaag gggctccggt atcgcagcag gtttttgatt tcagagagaa
    25141 gctcaaagaa gagttctact ccatgagtaa atctaactca gtagtgcaag gggagccacc
    25201 gcaaggggtg actgcattcg ttgctttaca attcgtttct gaatcggaaa gcagaaggat
    25261 gtccactgac gtgcaggtcc tgaacgctgc tattcgtgac ctttacgaca tggatttaaa
    25321 gcttgcggcg cagttctata agcctgaaga cgaacgcact atgatgcttc tcggtcctga
    25381 tggaaaatgg ttaaccaaat cttataatcc taaatcactt caagggcctt tcgcggtcat
    25441 ggtgcaaaac caaactgcat tgccggagtc tcgtgcagtc cgtacgcaat tcgtgctcga
    25501 tatgtcagag cgctatccag ggcttcttcc gaaagaacaa gtcattgaaa tgctaggtct
    25561 tggccagtct gagaaattca tggatctcgg atctgcggct gctcgtgcgg ctgaggaaga
    25621 gcaagaaatt attttagatc aaggtcagca aattgaaccg gctgaatggg aagatcacat
    25681 tacgcattgg aagattcaca ccatgaagat tcaggacatc ggctttaaga cagccacaag
    25741 tccagagtct caggaaatga tgaaggatca tattctcgcc acagaaatgc tcatgattga
    25801 gcaggctaaa cgctctcctc aattgactca actgttgttg atgctcccga acttcccaat
    25861 tttctacgtc gattctcaag cgcaaatggc agccggaatg ctgacaggaa ctattccagc
    25921 agtcatgcaa ggggctcaag gcgctatgca agcaatgagt ccaccgatgc cggaagagcc
    25981 acaacctggg gctcaaggaa agccgatgaa ctcaagcccg agtgatattt ctcaaggcgt
    26041 aggatcggaa ccaacaccag cgggcgcaaa accaacaaca gcataaaagg agacaatgaa
    26101 atatggcaga ggcagtaatt acaccaatga ataattcccc agcagcaacg gccacggcaa
    26161 aagcgccgga catggcaggg gacgcaccaa agacttttga tgaacttcag gcagcgattg
    26221 acggcaagaa agctgaagat cgcaaagcta aaaaagaagc gcaggaagca agggagccca
    26281 aaggcgagac gaaagacaaa ccaacggaca tatctcgcgc agactccaag ggcaaagaag
    26341 cgaagtcgaa agacaaagag cctaaagaat caaaagacaa agaagaagat gattcaaaaa
    26401 aagaagaacc aaagcctaag aaaaccctca agtacaaaaa aggggaagat gaggctgaga
    26461 ttgatgaaga tgctatgttc acgcatctta tcaatggcca agaggtccag gtcagtgcgc
    26521 gtgatctttt aaataattat tccggcaaaa cagcatggga taagaagttc tctgaagtcg
    26581 atagaaaagc gaaagacttc gaaggaaaat attctaaagt ccaacagcaa ttaaagggac
    26641 tctttgaaga acaagatcct gagatgcgta tttggaaaat ggcagaagtt gccggaatga
    26701 atccgataca atttaaaaag ttttatcttg acgataatat ctcactcgtt gaaaaatatt
    26761 attcaatgac ggatgaggag agagatagct actttctcca agaagaaaat aaatatcttc
    26821 gtcataaaaa tgagactgta gcccaagcag aaaaaactcg gcaagcccaa gcggaactga
    26881 gtaaaaaatt agagggatta cggcaagctc accaagtatc tgaagatgat ttttggagta
    26941 cctatgagtc tatcgcggcg gtggccgatg atgggaagct cgaacaagac ggtataaaat
    27001 cagccaaaga catcactccc gaaaaagtaa ttgaaattta tcagaaggaa catctgttca
    27061 gcgcaggcat tgaggctatg aaaagcacaa gtattaagcc tgagctgcaa ggtaatgcct
    27121 taaaagagct tgtcgatacg gcatttgctc aaggccttaa acctcaagat attcccgata
    27181 tgatttctga aatttggggc gttaaacgca gagaaaacaa ggtggctgaa aaagtgaagg
    27241 agcgcgaaga gtttatcagt ggcaaaaagg aagttgctac tccgaaaacg aatcgtgaac
    27301 caatgttctt tagcgattta taagttcttt aaaaattaaa tattaacaat ttctttgctc
    27361 cgtaactggg gccggaggtt tcaccatggc tcaaatgagt ctgacggcgt cgtctgcgct
    27421 gtttaaaaca aaattcggca agatgtccga aaacgcttac aattcctata acccaatgct
    27481 tggtactatc aaaaaacagt acgattttac cggcgacgaa atgaaggtag cagtaccgac
    27541 tttcttctca ggtggtgtcg gttcaggatc tcttccaacc gcaaaccctg cgtcagcagt
    27601 gaaagcaaca ctgacagcga aaaaagttta cgcgactact gaaattgatc gtgagtcttt
    27661 aaaagcttct tctaatgatg aaggttcttt tgtaaatggc atgaaatggg tttctgagaa
    27721 aaccgttgaa gcctacaatc gaaatgcttc tcgtattctt ttcagtgacg gaactggtgc
    27781 tttaggtact actacagcag ccgatttcgt tgatgtgacc ggaacaactt ttggagtggg
    27841 tgacacagct tatgctcttt tcactgcagc ttcttgggta gaaggttatt gggaagaaaa
    27901 tgactatgta aacgtgggca cagcagcaga cgttctagaa gtcatcgcgg ttgataacac
    27961 tcttcgcaaa gtcactttcg agaagatttc aggaacaact gacttcggaa ctggtggcgc
    28021 tggtacttca aaaatcatct acatgcagaa ctctaaggac aatgatccta tgggcttaaa
    28081 aggcgtgtgc gatgctacta cttccactct ttacggagtt gatgtggctc gtcgttggat
    28141 ggcgactcag atcgcagcgg caagcgctgg tatctctgaa gacatcatga atgagcttgt
    28201 aatcacaatc caaaagaaat gcggtaaggc tcctaagaag cttgttacct cttacactca
    28261 gtatcgcaag atttctaact tccttgaaga caagaaagtt taccaaatcg aagcgcgtgc
    28321 tcctgagttg aaaggtatcg ttggatggaa ggcattgtct tatatgtctg acgctggtga
    28381 gatcgctatc atcccagatc gcatgtgcgc agaagatcgc gtttatggcg tgaatgatga
    28441 ctacatcact tcttaccata gaccagactt cggttggttt gatgatgatg gaacagtgtt
    28501 cttgagaacc tcaggtgatg cttatgcagc tcgctacggt ggttactacc agaactacat
    28561 catccctagc ttccaaggtg tcgttacagg tcttgcaaca taattaaaaa cattgaggct
    28621 gcgcggggtt tttgtctttt tccccctgcg tggccttttt attggaggct taaatgtcta
    28681 gcaaacaagt cctgtgccct cagagggaga tgcgactgat gtctgtcaaa gttgatggca
    28741 caggtacggc ggctctatca gggacatgtt ctaacaacat gactctgact gacaacggta
    28801 cgggtgatta tactttgact tacaatctac cgtttttgcg tgctcctgaa atcgtagcta
    28861 ccgcaatcac ggataatatt tactgtaaaa tcggcgcttc ggctgtcgga tcttgtcaga
    28921 ttctaacaga gaatctttca ggcgctgcaa ctgatgcaga ttttcacgtc atcatcattg
    28981 gtagtgatac tgccgacgca caataggggg aattatgaca gcaggatatt ctgttagaaa
    29041 attccctatt aagtcgccac agttcaaacc gcgcttgatg acctttgggg taacaacagg
    29101 ccatgcgcag gttgaaactc tgactttccc agcggtggca ggagcaacac aaggtgactt
    29161 tgttgttctt tacaacgagg caggacagtc gattgcggcc tacattgata ttgatgcagc
    29221 aggaacggct ccaacatcgc cgctctatac agcagcaact tataaagttg ctatttctgt
    29281 cgcaactggt gacttggcgg cagccgtcgc aacagcgttt agaaccgcag tgaatggcgt
    29341 tttgcctgat gtgactttta ctcttgcttc agcaacgtgc atttgtactc aagacatcgt
    29401 cggagattgc tccaatgctg ttttgaaaaa ctcagatgga acaggtgttg gctcaatcac
    29461 gaattctgtc ggaactgaag gccttgcggc tgtgatgacg acaggagggt acgatggaac
    29521 acttactcaa actgcgactg gggtttacac aatcactttt gaaaggccat tcctcagaat
    29581 acctgagtat ggaatcacaa ctgtgactga taaccgtgtt ccgcgcattt cttcgggtac
    29641 tgttagcacg ctagtggttg aagtgagcaa tctttctggt ggcgcgacta ttgatggaag
    29701 cttccatgta ttagtcgttg gcagcgacgc caaggatgca attagaggtt aaataaaatt
    29761 taaggggctt gtgcttctcc tggggcactt gcttcttttt cactgagatg gcgctttaat
    29821 gcagccggag gaaatatgtc agttaaggga tatccatcaa atcaaaagct tattcaatct
    29881 gcttctggaa tcacgcataa ttatgcaaca gtagttccaa cggatgaata cagaaatgct
    29941 ttggacgctc ctcgattcgc atttcgcgta aacagtgcga cagttccgcg cacttgtggc
    30001 gcatcaactg gaaatgctct atctggccct actgtagtag tggacacatc aacggttgct
    30061 aaagtcgggg atattttccg agctgaaact gggaatgcag ccttcgttga agtccctatc
    30121 gtttcagtct caaccaatgg atttatttta gcttctagac ttagtactcc tccagcttct
    30181 ggagatactt tttatatttt aagatcagtc actcaaagga ctgatgaaaa cggttcgcag
    30241 gttgcgacta tagatacggc aggccttgcg acatcggcca agcaagatgc tgaagcggtt
    30301 ctggttggcg cagtcaatga gacagctccg gcgagcgata cagcctcaag cggtttgaat
    30361 ggccgattac aacgtattgc tcaaagaatt acttctttaa tcgctttgct tccggcttca
    30421 attggacaaa aggcaatgac cgcttctttt ccggttgttt tggcatccga tcaagcatcg
    30481 gttccggtgg cggcgacgtt atcggcagag acaacaaagg tcattgggac cgtgaatctt
    30541 tccgctgctc aaacgcttgc gacagtaact acagtttcaa ccgtcacagc ggtaactact
    30601 ctcggtacaa tcacaaatgc agttccaacg aaagctccgg tcaatgctaa cggttcaggt
    30661 ggtggacaaa cctctttgac agcgactacc gcttcgagcg aagcagctcc cgcaaatgcc
    30721 gttgggttta ttttgcaagc agatcccgcg aatacagatc caattcgata tagaatcggt
    30781 gcagcggctt cgacggtaaa tggaattcag ctcgcgcaat ctcaagatac tggcttcatc
    30841 ccatgcgcag cgacggtttc aatctgcgca actgtatcag ggactaataa atacgctatt
    30901 caatggattc tttctacata aggagaattt tatgcgcata attttacttt ctctcttaat
    30961 ttcttttcaa gctttggctg gacttcctcc gactacatca aaagtttcag gagattcaac
    31021 cgatcttacg acatacaaat atcagttccc taattttacc ggaacgcata ctggaacaac
    31081 ggtttctctt ggcgttaatg caatagcagg cggtggaact aataacggtt ccttggctgt
    31141 aaccaatggt ggcgtcgttt atactgatgg cacaaagcta atgaactcag gcgctggaac
    31201 tgccaatcag tatttggctt caattggtgc tggcgctcca gcgtggacaa gttttgttgc
    31261 tcctacgatt caaaaattta cctcaacggg tacaacaact ggttataatt ttacaataac
    31321 ggcaggaaac gcaacggttg gcgcgactta tacgaataat ggtcaaacct ttactgttct
    31381 ttcgactata gcggcagcaa caactctcta tacaactgca acgggtgcac cagaggcttc
    31441 tggcactctc acaaaggcga cgggcacggg tgatgcaact ttaacttttt cagcggcaac
    31501 ggcgacggct acttacacac ttccaacaag cccgagaaat ccagtttaca ttcgcgtaag
    31561 aatggtaggc ggtggcggag gtggcgcagg ttctgccgat gctacaaatg acggcggtaa
    31621 cgggggtgac ggtgcagcct ctaggttcgg agctgcgctg ataatcgcta atggaggcct
    31681 tggaggtata agtgcagcaa gttcaggcat tggtggtact ggtggatcag cctctttagg
    31741 tactgcagtg ggcacgtcat tgaccggagg taaggggcaa ggcttctctg gaggagctac
    31801 ttctcaaaga caggctggcg gcggcggcgc accttctcca tttggcggcg caggtggcgg
    31861 tggagcggct gctaccgctg ggcaaaatgc ctctgctaat actggctcag gtggcgcagg
    31921 tggtggtgct cctgttgctg gatttaatgg tccaggtggc ggcgctggcg gcttcgctga
    31981 tgctctcatt actagtccga gtgctacgta tcaatatgtt gtcggggcca atggaaccgc
    32041 cgggactgct gggacttctg gttttgtagg cggcacgggt gcagctggtt acatcgaagt
    32101 cactgagtat tatcaataag gaaatgtgat gaaacaagag ccctttaacc aagcactttt
    32161 tatcggtggc atccttgctg ttattttact agcagtaatt ttgagtacag gagtttagaa
    32221 aatgaaacaa attacagaga atactctcat tccgatctct ttagtaatga cgcttttggg
    32281 aggtgcggcc tggctgacag cggtttggta cgacacaaaa gcgaatgcgg tttcaattac
    32341 agaaataaag tcagaacaga aaattttggc caaggattta gtcgagatgc aaaagaaaac
    32401 tatcgaggat ctagccacga tcaaagttaa attaaacatt aaatctcagc aataaagcta
    32461 gagataatct gcgaggtatt aaatgttaga aggtaaagaa gctgaaggtt cgtttggtgg
    32521 atttggtggt tactcagtag acatcactga taaaggacta ttggaaatcg gcgtttcagt
    32581 tcgcattgat ttggttgctg aagctaaaaa acttgctgaa aaaacagcga ctccaattga
    32641 tgacactgca attgcgtggc ttgaaaaatt aatgtccaaa taaggagcac tcatgacttg
    32701 gctgataaac ttactggttc cagttatcga atggattctc aatttaggta ttaagcaatt
    32761 aatggcctgg tggaaggagc gcaaagataa ggaagatgtt cagaaaaaga aatcagccga
    32821 gttagagcag tctaaaaaag atttacaaaa agatttgaaa ggagccggag atgatgaaaa
    32881 agctcaagaa gacgctattg ataatttctt taagcgcact cgctagttgc ggaagcagca
    32941 gcctgccgga tgctccagaa atagagcaag gcgctcccat ttatggcact gatgccaatg
    33001 gtgtgatgtt tgtaaaatac tttcatcttg tgaattcaaa aaatcatgtt gaaacgaatc
    33061 tttctgttgc tcaaggcagg gagagaattc tttttattac agatcttgaa tcttacaatc
    33121 gatctgagtt gtatgttgcc gagcttgaac gcttggcaaa aaagaggtgc gaatgattac
    33181 gcaaaaacaa aaaataactg gcactgtggt agatagtgaa gccgtaactt tcgacatggc
    33241 aaatctcact gctgttgaat tccaggttac gactaccgga acagcgacaa gcggaactta
    33301 taaaattcag aaatctaatg atggaactca ctgggttgat attgcttcag ctacaggagc
    33361 tttcaccaca gctacaactt tgatgacaac ttgtacagga gtcgtggcaa gccaagttcg
    33421 tttattgtgg ggacttgcaa gcggaaccgt gacctatgta atgtacattc aaggtaagga
    33481 gttggtctaa tgggatacat gaactctagt tttggaattg atacaaatag agactatctt
    33541 tacggagact ctcaagatcc atttgatatt aataaacctc aaggccaagg ttctttgggt
    33601 agcgaggcaa tgaataaaga aatagcaacc agatttactc ctgagaatgc ggccgtaact
    33661 ggtggcgctg gattcttagc tggcggccct gctggcgcag cagtaagcgt tggtggatct
    33721 ctccttacaa actatcttgc gcaaaaagcc gcgacagagc gccaaaaaag acagcaatcc
    33781 gttcaaattg aaaatgaata cacaaaaaac atgggtgatg ccctcactca acaacttgcc
    33841 gcttatcgtg gcgctttaag gtaggtaaaa atgaagcaag caattgatat gatgaaaaaa
    33901 gcaatggcta tgatggaaca agcgcagaga cgaatgaact cagcagaaga tgaagaaagc
    33961 gaagaagtcg aaactgatta tatgccaact gaagacagtt cagattcttc ctttggaaat
    34021 tctaactcag gtggtgaaga agatgatctg gaagaacgca aacagaaaaa aaatatgatt
    34081 attgcttcga tgaaacgaaa acaatactaa agaggatttg aaatgagacg tttagaaact
    34141 ttgattgata attgtcgcga aataaccgga aacaaccgct atgattccga ttcggggcgc
    34201 agtcaaaaag tattcgtcga atatttcaat aacgctgcga actctttagc tagagaaata
    34261 attaatgcaa aatcgaagtt ctttgttaaa gagaaaatag tcacggttgt ttcagggcaa
    34321 agcctttact catggcctaa tgacattttt ctttttaatg tggacacaat ggaatggtca
    34381 acaaatggaa gagattattt tcccttatct aaagggatta ttaaggatcg ctttaatgtt
    34441 acttctggca ctgcttttag ttatatccct agaaacgacg gttttctctt aaatccaccg
    34501 caaactagtg gatatgtgag aataaattac atcaagcagc cttcgcgtct tgaaaaacgc
    34561 tctggaaaga tcacagcaag aactatggct ggacaagtcc tttcggctct tacaatagat
    34621 tcaacggaag caagctttga tcctacttac ataagccgag ataattatat ttctgttgtt
    34681 gataaatttg gggcgcaggt tgcaaaacga attgttttta cctctgttaa ttcgacaaca
    34741 ggagtgttta ctctttcgcc gattactcta gatacaggtg agacggtttc tgttggtgac
    34801 tatgtttgcg tgggactaga ttcttgcaat ttgcctccgt accctacaac atgtgaagat
    34861 tacttgatgc tctatgtaga gtatcaatca aaatatggag atgcttctaa gtggtccgct
    34921 gaaattaaga acaatctcgc agcggtggcg ggccaaataa ttacttcgtt tggactgatg
    34981 tctgatgata tcacgcaaat tccaattact tgcacagatt ttttataagg ggatttcatg
    35041 cctcaattta ccgataaaca ttatgctgac tttggtggtc ttgataccac gactaatctt
    35101 cttaagatgg acccgaagac tcaacgtcga ggctcaaaga attttcgcta taacaacgat
    35161 gatcaactta ctcaacgata cgggtttcaa cacattaccg aattaagctc tgcgtttaaa
    35221 tttggcttgg ccgaatataa atacagagat ttaaacacag gcgaatcaaa agttgaaatt
    35281 cttggagtcg gatcagatgg atttttacat cgcctccgaa acacaagact aaaaattgtg
    35341 cgttcgtctg gttctgtcgt ctattattca gttttttata acggtgatga ttggaccttt
    35401 gctctttatg gttcagcgat ggatgttatt tatagcactc attatgacaa tacctatacc
    35461 ctcagtcagc ttgtgaccta cgtaaattta tcggcaatta caggaatagc gctttcgatt
    35521 gtagatgaaa atgacagcgt cgtgacttgt gcaatccctg cgtatcttat gaataccgtc
    35581 attgttaaag agtttagcac tgctgtcacg aatactgaat acaatgcgct ttattacgat
    35641 gaactcgtgc aatgtccaag ctcttctgct ttatttcctg aggtcttaga ctcagcaata
    35701 acaagccttc cagattttga aggtctcaca tcaacaaatc tcaataactg tatatatctg
    35761 acttgcggag gattccccat aaagtacgac ggcaagtctg cttatcgcgc tggtgttccg
    35821 gcagtgcttc cgcaaatagg aagtaatcca ttaaatgctt ctggaataag cgcagcacaa
    35881 gcgacgacta ctttagaagt tgttttcact atttccccag ggagtgcgac atttggagca
    35941 acttacacaa acaatggagt aactttcatc gtatcagcga cagttgtggg tgcgactagc
    36001 attctgatgc ttggagcagg gggccctaca gcttctggca cactgacaaa agcatcaggg
    36061 gcaggtgacg cgacacttac tttttcagca gtggatcggg ggctcacagc aggatcttat
    36121 gcgtacaaat tcaaatttgg ccatattgat tcaaatggag ctgaaaattt aggtgattac
    36181 ggagtctcta attattttgg gactaatgga tatattcaaa gccttccaag cggagccgga
    36241 cataattcga taactcttac ttattatgga tttgtaaatg acagcaggtt cccagtttat
    36301 gcatgcaaag taaatggtgc gcagaacatt gcaactgctg gtggcacaat taacgtcgat
    36361 tcaggtcaca atataaaggt aggaatgtta gttcaaattc ctatttcaaa tgctcctact
    36421 ggatttaatg gatattctta tatcataagc aaagtaacag cagtcgcagc gacaactatt
    36481 actcttgcaa gcggtagaac tagcggaatt tatccttttg taactgggtc aacgacactt
    36541 atagttgata atcaaattct tcaagctgga tacagggaaa catattttga caatacaatt
    36601 actgatattc aaacatcagc cagacaatgg cagccacaga ctattgttgg ttcattctta
    36661 agagtttata gaacaaaagt aggacaaacg accgggccat tctacaagat gagagatctc
    36721 cctttgcctc ttggggcaat gggatcaact tcattcactg atattatttc agatgcaaat
    36781 cttatagaag aattcgatga ctcagaaggt gggccgcttc cgcgtgcctg ccgctacatc
    36841 ggtcaatggc aaaagcagct tgtgcaaggg ggacgtcctt acgataccac cttaaaagat
    36901 gactattatc cttcttatta tgctgccgta aatttagtca ataactggtc aacaaattca
    36961 acagaagaca gattttacat taatgaagag accgctctct gcgatttcca gagcgtctat
    37021 tgggccgatc cattaaatgc ggaggctttc cccacatcag gtcttaatga agaagatttt
    37081 caaggaaaac acgccgatca aattaaagga tttggcgaga ataaagaagc gctctttgta
    37141 atgaaagatc ggacagcagg aatgcttacc gggactcttg ccacaaacga tattcaaaaa
    37201 gagattcttg agattgatgc cgggtgctca tcccataatt cgattcagga agtaaatggc
    37261 tcgctggtat ggcttgacgg tgttgcagga ttttgggcct gcgttgctgg aagattgcca
    37321 gagtattttg gctggaaaat ccaagatgat tttagaaaaa acaatcagaa agcaaattct
    37381 caaaaattat taccaggacg cgcaaagtcg gtggttaacc gcattgactc gcaatacatt
    37441 tgctatattc cagcgggata tgtcagtgat gttgcaaccg gagatcccag ctcttactca
    37501 atcatgtttg tctttgattt ctctgcaacc gaggacggca agaggaattg ttggtatcaa
    37561 tggcaggacg ttagtccaaa tgcaggatta ttatacactt cgaccgatga gcttattttt
    37621 tcagaaaata gagctgcctc ttatagactg tggaagcaaa aaagaacagc cactaagtac
    37681 gacatggccg atcataatgc tgctattgag tttaatgcca aaaacgcatg gctcaactat
    37741 ggaacgccta ccattgataa agacttcgtg aatagttgga tcaattcagt ggaaggcggc
    37801 ttcactctga cagttcagca gtattacaat tacatggata ccgcggcgtc ttcctacaac
    37861 gtgacgatgc caacgggtac tagcaaggtc tcagtgaaga aatcagctaa tctcaatgtt
    37921 cctaaacttt cagcactttc tctcggcttt tatcacaata ccaagaatga actagtcaca
    37981 atcgacggct gggaagtggt gcttgcgcag gattatgatc ctggggagcc taaaatttaa
    38041 tgagcaacac gagaattaag tcgttagcaa aactgagaga ataccgcgct ggaatggata
    38101 tggaagaata tctcagcaca gaattcttgc aaaatctaaa ttttattgat cagaaattaa
    38161 gaagtctcga ccaggctgtt gtcgttaatg acatttgcgt gcgctatact tcagcatctg
    38221 gccagtccat accgagcagc gttctcactg ttttaaattt cgaaaccaaa gactttgata
    38281 gtgactcact cgtcaccgtg ggcacgtcat ggcgctttaa agcgaaagaa gatgccatct
    38341 atgaagcctc tggttttatc gttttcactt ctggaggcgg ctggggagta ggcgaggaag
    38401 ttgccgttaa tattcacata aacggcgtgt cctatgcgag aattggcggc gtaatcatgc
    38461 aagcagcgca tggaacttat gtcgagattc cactggtagc ttgtccgttt aagctcagaa
    38521 ccaatgagta catggacttg agggtttacc aaagctcagg cgcaagtttg tcccttcttg
    38581 gtggaattaa ttggatttca atcaagaaga ttggtgctta ctaatgacaa ggcacatggt
    38641 tactatttac aatttatttg atttcacttt ctttgctctt tagtcggagc cggaggtttt
    38701 agaatgggca cagcttataa atatgaccaa aatggcaacc caataccggt tggcgaagtc
    38761 attaatggaa cttatcaagg agcttggttc gatactcctg agcaagcaaa atctccagcg
    38821 gtttcaaacc tagataatga ttacagcaaa ggctacactc gcggtaaaaa agaattctac
    38881 gatgatcctg acatgcagga tctgaaaaaa cgcaatgaag atctcgcaaa gggctatgat
    38941 ggcaatactc tcggagcaat cagaggtgaa gccagagacc agatccaagg tcagagatca
    39001 aactacatgc ggcagcttca aagtcgagtc ggcaaagctg gtatcggtgg tgcaaggggt
    39061 gcagcgatgg ccggggctgc tgacaaaggc ttcatggact caacggcaag ttctgaaaga
    39121 aagatggcta ttgataactc gaaccttcag cgtgaaggaa ccggaaagct ccaggatttc
    39181 atctttagac agaaatatgg aactctcggc tcagcaatcg gcgagcaaca ggccgggctt
    39241 gcagagcgtg gtaaatacgc agcagcccag gcgaatcaaa gcggcggcgg tggtatgtct
    39301 tggctttgca ctgaaattgc taagaatgaa aattggacta tgtcagattt aaaggcactg
    39361 aaaaagcttc tacttttcac tgaaaaacac gacaaagagg cttctgagag ataccaaaaa
    39421 gatgcaccga ctttgattgc tatcatgaaa gacaagtctt ttgattttat gcgccttcag
    39481 cctacgatta aatgcattat ttctcttgtg aagattggcc ttatagaaat ggcctacgag
    39541 aattacaaaa atatgacgat ggatcttgta aatacttact ggggtgatta tggcagagcg
    39601 tgattattta gcagaacttt tacttaatca aagacttcca gagatcaacc tggctgatga
    39661 agccgtacaa aaagtaagaa acatgaaggc agcggcggct cctcctccgg ttgttgctcc
    39721 aagaccacgt cctgctgctc ctgttgatga tggcggtatc gatctaaaga agatcgaaag
    39781 ccaagcgatt tatcaggccg cgcttcaagc aaagcaaaaa gcagcggtgg ataagataag
    39841 agcaatgact gctaagcctt ctccgactcc agtgcctacg cctacgccac aagtgacgcc
    39901 aactccagag cctcaaaaga gtatggtaaa aatgccagac ggaaatatgg cccaaaggga
    39961 aaatgaaatt gaaattagcg ctgatcctct gaatgaggaa gattggatac agcctaaatc
    40021 tacagatcca aagtctcttt cggctaatcg agattcttat aatgtaatcg gttacaacct
    40081 cgataagact atcaagcaac aagatgcttt agtcggacgc gctgaaatgg cgcgaaagcc
    40141 ctacgttgat agactcgaga aaatggttga tgcatacatg caacccgtac caaagtttca
    40201 atcttcatac gatgagtatg caaagaaaat gcttgagcaa gaagcgacag agaagagagt
    40261 agcgcgtcct gattttcaac ctccgcaaag gcaagaagaa gaagatattt taactaaagc
    40321 aatttacgct ttcggtccag gtctgggcgc tatggccctg ggcgaagacg cggcgcttgc
    40381 ggcccctaca gcgcagaagc aagcctatgc gcttcgtgct tctgacatcg ctcagaaaaa
    40441 caaagagtat gaccaagagc ttgcggctta tagagcaaat gcagtcagag aaaaagaaaa
    40501 gggagttaat agctctcttc gtggaattgc tcttaaactt ctcgctgaag gtgaaagaga
    40561 cgatttcaat aaacagcaag acactcaaga caagcgaatg ggtgaagcta cgaagatctt
    40621 gtcagggctt tctgctgatg aatccaagcg ccttgatgct gcaattcagg atcggcagga
    40681 actgctaaag gcgaatgtcg ataaaggtct gatttctgta aaagactatc aggatcatgt
    40741 tgaaaaatta gcggcatttc aaaatcaaaa agatatcgct gatgcaaatc gcgatgctca
    40801 aaataaacgt cttgatattg aagaggctgg aagaaatcag cgtgccaaag agtccaatgc
    40861 tacaatgctg aaagccaccg gaatgagggg tgatgctcag gcgaatgttg ctggaattcg
    40921 ggcagcggcg aaaggttcta aaacaggcgg aaaaccttta accataagcg ctgctgaaag
    40981 catgtctaat gcaaacactg cattgtcttc gctggaagat atcaattcaa ttgtaaccaa
    41041 aaatcctgaa atgttcggta aaggtcagaa tatcttaaat cagatcgcaa ttgcgacagg
    41101 catgggtgat actggcacaa aggccgcatc cattgatgcg gaactaggag ccatagctca
    41161 gcgtatcggt gtttatcttg agggtggaaa gcttgctgaa ggagacatca agcggtatcg
    41221 caaaatgctt ccagagcttg gtgataagcc tgaaattgtc caaaataaaa ttgcatctct
    41281 caaaaggttg ttggaacaga aacataacaa cgatttagag tcatatagag cttcaggata
    41341 tgatgtttca agatttgaac gaaagcaaat tagcgcagga atcaccgaag gcgttggaaa
    41401 gaaaaacgag cctggtaagt ttccgatgac agtcagaaaa ggcactcagt ctacaacggt
    41461 cgaaaatatc aaagaactcg ttgaagccag aaaagaaggg tggaagtaat gggatggaga
    41521 gaccggattc aagatgaagc tccagcgcca acaaagtcca cttggaaaga tcgggttcaa
    41581 gacgaatata aaccagatct ttcggagttg atgaattacg aagatccaag caagaaagat
    41641 tatcctggca cagttcgcgg agctataaaa gctcttccgc ttgcagggac ggtagcaggt
    41701 ggcgcttttg gaacagtggc aggtggtcca cttggtccat tgtctggtcc ggttggagct
    41761 gggcttggcg gtgcagcagg taagtcggta gagaatattc ttgatcatta tctttatgga
    41821 gacgaaaaat catataacga tattttggta gatcctgcga aagaggctct gaactcgagt
    41881 acaatggaaa tgctaggtcc agtaacagga gaattaggca accttggagt taaaggacta
    41941 aaaaacgtat catcatcaat gtctaaaatt ccaaagcgtg caatggaagt atatgcagag
    42001 cgagggccgg aagtagcaaa aatttctgca aagtctgaga atgatttagc tgaaggcgca
    42061 gaccaaattc gacaagatat tcagaaaagt gttggatctt ttaaatctca gcagaatgcg
    42121 aatatatcta actcacttga cgccaatggc agacaagttg tcgatatgtc tgggaccatc
    42181 aatgcgcttg aagaaaataa ggcgaaacta aatccaatta tcaatgctga taaaatcgga
    42241 aaaatacaaa atcagatcga tttaattaaa cgcgtaggga tggacacgaa agacgggaaa
    42301 ttcattatgc tagcgcgtga tgcttacgac gtgcaaaagc atcttcagga ccaagctgaa
    42361 tatttggctc ctggtcagct atttaagaaa aaagattttg tcgatattac tttccagcgt
    42421 gcagcttcta gggcaagaca agctgttggt agtgcagctc ctgaaatttc agcagcaaac
    42481 gcagaactag gaaaagttcg ccgtatggaa ggaaatatca ataaaaacct tattgctccc
    42541 gataagccct atgctggttt aatttcagca ggaaccggcg aaaatcaaat aaatcgaaga
    42601 aatcttcagc gcatcggtga cgccgtcggc gctgattttg ttccggaagc ggaaaattta
    42661 gcggcagctc atcactttaa tgacgctggt ttcctgccaa aagtacaaaa tggagcttca
    42721 atggcgcctt tgattgcagg aactggtgga attggtgagc ttgttagagg tgatgtcagg
    42781 actggccttt caagtctggc ccttggtgcg atgggctcac ctatagctat caagtcagcg
    42841 attgatacag caagagttgc taagcaggtg gcccctatag gtgtaggtaa tgcaatgcaa
    42901 agcattccgc aattcttgca acactcacta gggcaagagc cagagatcga aggttacaag
    42961 atgggtgaca caattaaatt ggatctagcc accgatgcgc agaattttga gaatgaaatc
    43021 aaaaatgatc ctaaattgcc acctacgcaa aaagcaaaga gactcaacct tttgcgcaag
    43081 catgggcgaa tttatattgg gaattgatca tcgactgggg ccggggcccc tttgatcatc
    43141 aggaataagc gaggaacaat tttcacggcg ctatatacga aaaaaataaa tgctgctatg
    43201 gctgaaagat aatacatgaa tttatcgaaa gcggcgctgt cgaacggctt ttcttcgctt
    43261 ttcgatttgc cgtaagtgtc cataaggtct ttggtggtta aaaagcgttt gctcatgatt
    43321 cctccagggc ggaggcatat cataattaca tgggcattta tagactgttg caattatttc
    43381 acaactcgat caaggaatga ctttgatttc acgctagcag gcactctctt gctagcggct
    43441 tcgatcttgc tcaggatatt gcagttttta tcttcaaggt aagatgctgt ttttatagtt
    43501 gaatttcccg atattggaga gagggaaaac ttaacagatt tgtcatagtt ttgaatcaaa
    43561 agctttccat tcatagctga aatataaaga tcctcttgct taatacgatc gcaggtatcg
    43621 taactatatt taatatggaa aacgtcccct tctctagtga aagtaccgat ggattttctc
    43681 attaccgctt tcattgagtt tccatcagac tccaggtacc cgtcttgaaa ttcaatgttt
    43741 ccattgcttt gaatattaac aatgaatcct gttgcactgt attgggttga tcctactttt
    43801 tcataatacc attcgccata aataggatct gccgttgccg ctgaattatc accgccattt
    43861 ttacatgctg aaagaacaaa gattagcgct aggattgtgt atttcattta aacccctttg
    43921 tcttataaac atcgggtgtt tagattgaaa ctttagttta tttcacgcgg aaaagatcgg
    43981 tctctttaac gcgcagacat ttagaaagcg cctgtatcac tttgagccgt ggccaggact
    44041 ttccagcctc caacatctga atatacctgg gggagcaatc gactgcctcc gctagctctt
    44101 cctgagtgag ttttctcttc tgccttaaac gcttgagatt gctgctaaaa acaagttgaa
    44161 ttgactgcat ccattcattc tgaggcgaaa gctaacttcg taacacgaag gctaatttcg
    44221 tgtgaaatga ttacataaat agatatgatt tagaaatagt gttcgattta taagcaaaat
    44281 ctgatagaca gggcccaagt caaaactaga ttgaatgcag tacgttacag gagtggtatg
    44341 agtgacttgg agcgggccaa aaggctggcc tatgagctat tttgtgagct tgaccggatt
    44401 ggcaagatta tccaagactc cagaactagg cctgagggcc gtttcagtga aaccaaggac
    44461 agacctaagt tgatcctcat taagggcgtt gaggttgacc ataatctgac cgagtagctc
    44521 ggtttttgat tcttgcggtt tttctgtagc aaacaggtcc gttttgtctg acatattcac
    44581 tgacttcaag aattctgtcg tcacgcctag agctttagct aaatctggta atatgtcgga
    44641 tttaactata gatctatgtc cctttattag ccgatttaga ttctgatagt taatcttagc
    44701 atctttggct aattgacgtt gagaaatatt tttctcatcc atcagcttga aaattttact
    44761 aatcacaatt ttatcaatat ccacgcttac ttatcctaac attactacgc gtcaaacgag
    44821 tatttggtgt tatggctaat atttgttgac agtatttcga attatggcta atactcatta
    44881 gcagaggtag cgatgaattt gagtagaatt agagacgaaa ttgataaaag gggcataaag
    44941 caccgatggc tggcaaagca aataggtgtg cacccaacga ctttaagtcg cttcctttgt
    45001 ggaaaaatag ggttggcaga agaagctcaa gagaagctct tccgaattct aaaactaaaa
    45061 ccagaggacg taactaaagt ctcctgatcc cttagagggc ccgttggcgc ggaccttctg
    45121 taggaaccac gacgcttaca atggattacg gaaattttga attgatttag cgaaacttga
    45181 aaattttgca taggcattgg tttgcctgga ggagtgagag aatgagttac ccaatggttg
    45241 gatgcggaaa atttgacgag cgggcaagtc gaattgctaa tctggcttac gactatttga
    45301 acttagctca gaccttgaaa atctctcatg agcgcagaaa gcactactat tctcgctatc
    45361 acaagctttt aaacgcgcgt tttgaccaca tcagaggtgt ttatgcataa aaactactac
    45421 cgctacacat tcgagaaatt cgacaaggac ggcaatccaa cgatccaatg caactatgac
    45481 atcactggaa aaattggcga gatttttgtg tattccggcg aagtcgatga aatggtgaaa
    45541 gttgacctcg aggttttcga gcgccgtcat gtttctaaat tcgcggaatt gcagactgaa
    45601 gtttatttaa tagttaaaga tcaggatttt atttcatccg ttagatacgg gacataggtg
    45661 gcaagatgag caacatcact cttttcgaaa aaacaaagtc actcattcat gcgcaggcca
    45721 agaaatttct tgagcttgca aaatcccata aggccgtaaa ttttgaacag gaagcaagct
    45781 ttgcacttca ggctttgcag gcaaataatt ttctagcatc cacggcggca aataatccta
    45841 actctttgat tgatgctatt cacaatgtgg caagcatcgg tctctcttta aacccagctt
    45901 tgaagcacgc ttacttagtc cctcgagacg gcaaagtttg tctggatttt gattatcgag
    45961 gtctggctca cctgtcctat gaatcgggtg ctgtgaagtt tgtgcaggct gaaatcgttt
    46021 atgaaaacga caggtttgtg ctacgcgggc gcggacaaga accaattcac gagcgtgacc
    46081 cctttagtca agatcgtgga ggagttagag gtgcatatac tattgctaag acagttgatg
    46141 gtgatttttt ggttgagaca atgactattg ctgagatcta cgcgattcgc aacaatacgc
    46201 aggcctacaa agcttttcta gataaaaaaa cgaagtcttg cccttgggtt tcgcacgaag
    46261 gggaaatgat caaaaaaacc gttattcgca gggcctcgaa aatgtggcct aaatcaaatg
    46321 ccgggcggtt tgaaaaggcc atgcaggttc aagatgaagt tgaaatgatt gacgtcactc
    46381 cgcaaagcaa agaaccagaa aagccagatg aatcaccaaa gaaacttgaa gaaatcaggc
    46441 agcttttgac tgcgattgat cgctcagagg agaagtatct tggacatctt gagcgcgtgc
    46501 ataaacgcga tttaaagacc ttggaagatc tttcaatggc tgaaattgaa caagcattaa
    46561 ttgaacttta tcaaatttca tcaaaggcaa ataaaaaacc ggagaataaa aatgaagtcg
    46621 cttaaagacg ttgaatcaca aattaaaact ttccaggata aatttggcac tgacattatg
    46681 cgtgctgaac agggctctat ggcgtggctt caaactcgcc tgggtgttat cactgcgagt
    46741 gaagtgagtg ctgcggtggc aaaaatagga tctgaaaccc gcaatactta tatgtgtcaa
    46801 ttggtggctg aagtagccac tggcatggtc gaggaaatca attcaaagta tatggactgg
    46861 ggcaagctga atgaagatgc tgcccgctca agttatgagt ttgcaaatga cactgtgatt
    46921 gagcaagtaa atttcatttt caaagacgat acttttcggg aaggcgcatc tattgacgga
    46981 ttcgtaagcg ggcagaaaaa gctagtggaa atcaagtgcc cttacaatcc tacaaattat
    47041 atcaaattta ttgtggctga caaaatcaaa gccgaatacc aatggcaata tcagtttcaa
    47101 atgcgagtga cgggtgctga gaccgctgat ttcgttcaat acgatccacg catgattgtt
    47161 caaccgatta aggttttaaa agttgagcgt gatgacgaat atcaaaagaa gtttgatgaa
    47221 ttgattcctg catttcttca tgacatggat tcgatgttga aactaattgg cattgaatat
    47281 ggtgatcagt ggaagcgcat tgccgaaatg acgcctaaaa tagcatgacc ctattcacca
    47341 tcttccgcaa actcgacgct cccacgatct gcaaagattg ctttaagccc atagagccaa
    47401 ggccaaagag tgagccacgt ttgtgtgatg actgcgcgga agtggatcgg aaatatgatg
    47461 agattatggg gaaagagaaa cctgaatttt ataagatgcg gaggaattga gcatgactaa
    47521 actacaaacc gaagtattaa aattccacac tgattttgag gcacgctatg cggagttaga
    47581 ggcggcatac aaagataagt cgtatgatca gacttcctcg ctagcggctg gcattgtggg
    47641 ctttacgaaa gcgagagcgg attggctgaa gcgtgaagtt gagattgata ttgcttttag
    47701 cgagaaagaa taggcggtgc tcccgatgcc aattcggggg aatttgaagg agtcactcaa
    47761 gccactgata cgcaggtggt ccgcgtatgg aacccataat tagcttggga atttgagtga
    47821 ttccttgaga ttaaagcacc aggaagcccg ctgaattgcg gaatgggttc gtgagtcgat
    47881 caagatgatg agacttagtt caagcgggtt tttgggtgtg agctttatgg gattgtaatt
    47941 acagaggagt gaagtgtgaa aacaatcgaa gaacagcaga aagagtttaa gcagtgtggt
    48001 gatgactttg cagatgccgc ctttccttgt actttggaat ttacttacga agcgaatcct
    48061 cacatgaagt atgtcatttc ttctaaggga ttaagtaagc gtgaatattt cgcagctcaa
    48121 attatgactg gcatgatgac aacacctgac aatataggtc acagaaagat atcggcgcat
    48181 ttagcagtgg agcttgcgga cgaactcatt gaagcactaa acaaagaaaa agactaagca
    48241 cgtaaagggg gtatgaatgg acatcatagc aaatatattc tgtttcataa tcggtcttgc
    48301 ggcggcggtt tgttactatc atccgagaat tcgggtgttt gaagttgtgg ttgaggattt
    48361 gataattaga aatagcttga gagagatgaa agggaagaat gaaaaaaact aaatggatca
    48421 caacgaaagc agcgggtgaa gaaatcgggc gtactgccag gactatccaa gattattgca
    48481 aagccggacg tgttcaatgc gctcgctttg gccgatacta catgattgac agtaaatcat
    48541 gggaggaatt caaggcccgg catataatta aagcatcgta ggttatggca aattcaaagc
    48601 tcaaaaagtt caaagacaaa aaagggaatg aatatcctta tctttttatc gacgaaaaga
    48661 ccggggaatt ctatgccgtg aaacgaattc acttggccat taaaaagaaa cctctcggca
    48721 aagaattcac aaaagcgcgg tctcaggttt tgaccgcaat ccaagaaatg atgcatgaga
    48781 aacctaaaaa agtaggctct gcgctcatta aagatttcta tgaaataatg ctcgctgaaa
    48841 aaaaagccat gtcgataaaa gagtcaacgc ttcggcgcat ggatactgtt tggcgaaata
    48901 acattgaacc ttattggcaa tatttgactc ctgaggaagt cgatcaagat caagttacaa
    48961 aattcatgac ctggcatcga cgaaataagc ccggggttca attctcaaat acgtttaaat
    49021 atctgggcaa tatctttcaa gtcatggtcg atcgagacca tttaagcccg gggaagcgtc
    49081 cgaaacttat tatcccaaaa gatgagcaag cccatcacga caaagacaaa ggccgctaca
    49141 ttaccgacaa agagattacg gccgtacttg aaaaggccga tgacgtcact tacttgatga
    49201 ttcgaattgc tcatatttgt ggcaatcgaa aaatggagat tggatcactc gaaaaagagc
    49261 gggtgatttc gtcgggcaag cattacgtga tcgtatttga taccgatgac acaaagaccg
    49321 gcatggcccg ggaggttccg cttcccaagg atctgaccga gaaaattgcc gctcaaattg
    49381 catcaagcaa atcaaagttt ctatttccca tgcattctga tccggagcgc cacgtttacc
    49441 cgaaggttat tgatgacggt tgggatgctg ccaaggccgc cgctaaaata aaaggccgca
    49501 tgaggttcca cgaccttcgt cataccgctg ccagaaactt cgcaagtgct aatgtgaatc
    49561 caattattgc ctgcacaatt ttagggatga gccttgcgac atatcaaaaa gtttattgca
    49621 aactgaaggc ttcagacttg ataatagccg tagagcaaag tgctgcccga ttgggggcag
    49681 aatgagtcaa aaagctctgt cttgtttcgg atttgtaccg gatttaatag ccggattctt
    49741 gcaaatgcgc gtaattacac gtctaaatca aatttcaaaa gagtcttgta cagaccacca
    49801 attttcttcg gttctcttta ctgagggcta cagaaatttc agtgtagaga gaaaaaatcg
    49861 ttttttaatg aaaataaaat aagttcgttt agtaagttga ctatgaatga gtcaaagcga
    49921 ttcatagact cacaatattt ttatcgtaat tactaaagct tcaaatgagc ttccgatcaa
    49981 agtcataaat tttatgtcta atggcgtatc gggggtcgtt catgagaatg ctattgttgg
    50041 taatgttgca aagttttgtt ttaaccgtat ttttcaccat gatttcccaa gctgacgtgc
    50101 gcacttttcc taccaagaat tgtcgcgata acttttttaa tttgaaaaaa tatgagcgcc
    50161 gaagcgaagc gcccaaaagt tttaaataac ttagttcgtg ttgtaacata agatttcttt
    50221 gtgagaaggc cacatgattg atttttataa aatggaaacg ccagatggct ctaaggttca
    50281 tattcttctt gaggagctgg ggatgaaata ccagctccga tctccagaag ctgattattt
    50341 gaaaatcagt ccctatggag gggtctcggc gatcattgac ctggagggcg agtacagtgg
    50401 acagattcca atctttgaat cagaggccat tcttaattat ttagcagaaa aatctcatca
    50461 ctttctgggg gccaatgaat ttgaaaaagc ccaggtgatg tcttggctta tgtttcaggc
    50521 ttcggtggtg ggacctactt ttgctaatta tgagtatgct aaagagaatg ctataccctt
    50581 gatgctgaag cattttgagg aagaatcaaa aagacttcta gggatgatgg atgcgcgtct
    50641 tgctgagaaa ccctacttgg ccggagattt ctattcgatt gcggatatcg ctacatatcc
    50701 ttggattgcg gaaactttga aatctagagc ggagtggtat gaagccactc cttatcttct
    50761 ccagtgggcc gagcttatgg gacggcgtcc ggctgttcag aaggcttttc aatagtgatt
    50821 tttttgaaat cccataattt ttgatccaaa agctggctag gatttatatt gcttaaagat
    50881 gtcatgatgg aagcgccgat atgaggaata tctttttcca actcttgaat cagttttttc
    50941 atctttttac gt
//
LOCUS       DATBTE010000390         1622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2445884, whole genome shotgun sequence.
ACCESSION   DATBTE010000390 DATBTE010000000
VERSION     DATBTE010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2445884"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VIG33_14945"
     CDS             <1..194
                     /locus_tag="VIG33_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272185.1"
                     /translation="FLENFPVWIEPKDADIYPARPSILFDDSTRIYQIFPFFVKEPSF
                     EETKFRLIKRISVTVKFEN"
     gene            complement(357..500)
                     /locus_tag="VIG33_14950"
     CDS             complement(357..500)
                     /locus_tag="VIG33_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272186.1"
                     /translation="MNFFPFNSYFAVIMGARKIFKAKKRPPNGNAPLGQEGAFYFKEI
                     AKA"
     gene            910..>1622
                     /locus_tag="VIG33_14955"
     CDS             910..>1622
                     /locus_tag="VIG33_14955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEY8272187.1"
                     /translation="MKHNILLVDDNEALRNSMSAFLTDEGFFVRAVATGEEAIALVRQ
                     KFIPFSMAFIDFHMPGMKGPEVIRQIKEHNSNLLVFGLSGDDSDDAHNQSLDSGAAFF
                     ISKDTTDAKLLGILHRNCRDIEKKTRPLEICKSSENIKLIESVGMAGASNSLAEVARL
                     ILKFAPSKESVLIRGENGTGKEKIARAIHQNSPRRLMPFIAVNCAAIPESLIESELFG
                     HEKGSFTGAGKDRVGYFKAA"
BASE COUNT          496 a          312 c          322 g          492 t
ORIGIN      
        1 agttcttaga gaattttccc gtttggattg aaccaaaaga cgctgacatt tatccggccc
       61 gaccatcaat tctttttgat gatagcacca gaatttacca aatttttccg ttttttgtaa
      121 aagaaccatc gtttgaagaa accaaatttc gccttataaa gcgtatatcg gtaacagtaa
      181 aatttgaaaa ctaaaacttc agtcgagatt ttttacggtg aaatcgattg tattctcata
      241 cacatagaat cgctcgggca gaagttgctg ccttaaaaac actaaattgt tagctgataa
      301 tcagtaaaaa agtttcaggc ttttttaaat ttgaagctgg aagcgcggtc gatatcttat
      361 gctttcgcga tctctttgaa gtaaaatgcc ccttcttggc caagaggggc atttccattt
      421 gggggtcttt ttttggcttt gaagatcttt cgcgcaccca tgatgacggc aaaatagctg
      481 ttgaagggaa aaaagttcat agttcactcc aataagaaaa agtaattcgt tccataactt
      541 aaagccttaa aatcattccc aaaaaatgtt tagtgaggga atttaaaacg ccatctgctc
      601 gttcattttt tgaaaaattt ttaatcgaaa aatcctctaa ctcaatcgct tggcactttg
      661 acgatacaag tggcggttct ccgccacttc ttagacagcg caacttattg aaagtagttt
      721 attaatattc tagagaaccg cgcattttat aaaaaatgta ttttttttat aatgccgaac
      781 ataggcattg caaaaagtga agacaaaaag cctctgtaat cgttttaagt ctcgattaaa
      841 acattattct atttataaat tgattggcac tattcttggt atatggaaat tcaagaaggg
      901 aggccgcaga tgaaacacaa catcctttta gtcgacgaca atgaagctct cagaaattcg
      961 atgtctgcct tcttaacgga tgaaggcttc tttgtgcgag cggtggcaac gggagaagaa
     1021 gccattgcgt tggttcggca aaagtttatt ccattttcta tggccttcat tgattttcat
     1081 atgcctggga tgaaggggcc tgaagttata cgtcaaatca aagagcacaa ctcgaacctt
     1141 cttgtttttg gcctctctgg agatgattct gatgatgccc acaatcaatc acttgattcg
     1201 ggagccgctt tctttatttc taaggacaca accgatgcaa agctccttgg catccttcat
     1261 aggaattgcc gagatattga gaaaaagact cgtcccttag aaatttgcaa atcgtctgaa
     1321 aatataaaac ttattgaaag tgttggaatg gcgggcgctt caaactctct tgctgaagta
     1381 gcaagactta ttttaaagtt cgctccctct aaagaaagcg tcctcattcg tggagaaaat
     1441 gggacaggaa aagaaaaaat tgcccgtgct attcatcaaa actcaccaag acgacttatg
     1501 ccatttatcg cggttaactg tgcggcaatt cccgagtctt taattgaaag tgaattgttt
     1561 ggacatgaaa aaggctcctt caccggagct gggaaagatc gcgttggcta ctttaaagct
     1621 gc
//
LOCUS       DATBTE010000391         6023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_805840, whole genome shotgun sequence.
ACCESSION   DATBTE010000391 DATBTE010000000
VERSION     DATBTE010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805840"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VIG33_14960"
     CDS             <1..272
                     /locus_tag="VIG33_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272188.1"
                     /translation="KLVMIDPQKKLIQKGSAEQKVPKKIRVSDYKQLKRIAWQLKGSQ
                     ELSPQEVRDLYERNWRHIDLKTMDAREKKLLEMLLAAAGRERLLV"
     gene            complement(406..1443)
                     /locus_tag="VIG33_14965"
     CDS             complement(406..1443)
                     /locus_tag="VIG33_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY8272189.1"
                     /translation="MRSLIIFLISLSLLSCTKKQEAKPSSEINLAIWGNYLSAEVQKK
                     FTDETGIKINITNYSSNEELLAKIQMGASGIDMAVPSDYMVDIMIKLNLLEPVDKTQI
                     SNFKNLSPKFLAQSYDPTNAFSIPYAWTTTGVAVNRSLYKGSIKSWKDIFENPELKGK
                     FSLLDDGREVIGTALRLHGHSINSTDVKELNEAEETLLKVKPNVKTFTSDTIEILKNK
                     EVAVAHSFSSDALQAQAQSNGEIEFILLNDGGTRSIDNMVIVKGAKHKDNALKLIDYM
                     LKTENNLVFIKKVRCGPVLSTTRDLLPEDLKNNKALFPSDEQFKKLEQIHDLGKVNQM
                     YESIWTKVKTH"
     gene            1518..1952
                     /locus_tag="VIG33_14970"
     CDS             1518..1952
                     /locus_tag="VIG33_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272190.1"
                     /translation="MANFKIDFFVHPIALFPVALMAVNDHWLKYAYPGWFTGKLSDFA
                     GIFYFPIFILAMVIFVFHVVLTRRNLWLAILFTDFLLMLVKLNEPAARWIETIFAQYL
                     FRIQLIQDPTDLLALSMNALTYIYMKKYLTGAGEPAQSSLDK"
     gene            complement(1909..2337)
                     /locus_tag="VIG33_14975"
     CDS             complement(1909..2337)
                     /locus_tag="VIG33_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272191.1"
                     /translation="MNTTAKSWFTVLCLLTTSLVTVATSKAPPPKTGSDDVITAESKQ
                     YYVASNCPAALPQDQITVTNETISYPTNLQFADFGLPLTRLNFEMYPQVKGWVRGYQM
                     LCYLSVMEADSDTGLLHVYNCYENNRPICQVSFEQVLQRQ"
     gene            2588..2893
                     /locus_tag="VIG33_14980"
     CDS             2588..2893
                     /locus_tag="VIG33_14980"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8272192.1"
                     /translation="MDFKLSTAQEICYELGQRLKTQRLAKNVKQQELAEQAGISVGTV
                     KNLESKGQASLENWIRIIIALDLTQDLEALFTLKIRSISEMEKMEKLKSNKISRRAR"
     gene            2890..4119
                     /locus_tag="VIG33_14985"
     CDS             2890..4119
                     /locus_tag="VIG33_14985"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEY8272193.1"
                     /translation="MKKVDVFYSGWGEHWKLGRLADNGTKLLFEYTAEALEQGLELSP
                     LNLKLKSEAYGEFPTHQQRLPGLIADSLPDGWGLLLMDKLFRKQGLAIEEVSPLDRLS
                     FLGDRAMGALIFKPASESVAQPKGLSLLEIGTEVHKIVSEKASSALSELAILGGSPQG
                     ARPKVLAYYQKKTKQLSMTSVPTAHPWLFKFPAQQEHKEVCAIEALYAKLAKKAGLEI
                     KETEYFELSKNLSAFGTKRFDRENDLRIPTHTLAGALNADFRRSFSVDYLSLLRLTRL
                     MTKDEREVHKAFRQCLFNVIFNNRDDHAKNFSFLLDDKRRWKLSPAYDLTFNAGPGGE
                     HQMDICGEGRAPTKTHLLKLAEKSGIHIKVFEEILEEVTAAARQFKTEAKNWPLRTAT
                     IHTIHQTIQANSKRLKL"
     gene            4235..5233
                     /locus_tag="VIG33_14990"
     CDS             4235..5233
                     /locus_tag="VIG33_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8272194.1"
                     /translation="MLELSHISKTFSNQMALREIGLKIRKGEFFSLLGPSGCGKTTLL
                     RILAGFETPTQGRILLEQKDISGLLPPARPFNMVFQKYALFPHLTVHENVAFGLKMKK
                     LPNEEIKRRVDEMLQMVGLKEYAQRKPETLSGGQSQRVAVARSLVNQPKVLLLDEPLS
                     ALDLKMREHMQIELKTLQRKLGLTFIFVTHDQEEAFALSDRIAVMNKGGIEQVSTPQE
                     LYEQPQTRFVAEFIGHMNKLPAHLLPPHLITAAKDYFIRSEKIFFKGHSSAPMTVKAQ
                     VLQKTFRGDHDEWQVEVSDKTLLKLKTPVGAHTLSAGQPVEICFDEKDVFSFKDEV"
     gene            5233..>6023
                     /locus_tag="VIG33_14995"
     CDS             5233..>6023
                     /locus_tag="VIG33_14995"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8272195.1"
                     /translation="MKVRELTGPALFWYGLFLMGPLLWIFILSFQTRGTYGGIVWTPT
                     LANYAKVLEPAYFLIYLKSLILAFSTAAGTVFLAVILSWAVVSVDKKWRILYLAALVL
                     PFLINLIIRVYALRILLSYDGPLQMMLRAFNIEFDPFSLTSNNGLVFYGMITTYLPFA
                     VLPIYSALSRFDFTLFEAAVDLGATASQTFLKVVAPSLRGAMTSAFALVFIPALGEYI
                     IPDLLGGAKTMYVGNLLTEEFLKSRDWPLGSALAMTLIMVLLLML"
BASE COUNT         1671 a         1324 c         1327 g         1701 t
ORIGIN      
        1 tcaaactcgt tatgatagat cctcagaaga agctgatcca aaaaggatca gctgagcaaa
       61 aagttcctaa gaagattcgc gtcagtgatt ataagcaatt gaaacggatt gcttggcaac
      121 tcaaaggctc tcaggagttg agtccgcaag aagtgcgtga cctctatgag cgcaactggc
      181 gacacattga tctcaagacg atggatgcgc gtgaaaaaaa attattagag atgctgctgg
      241 cggcagctgg acgggagagg ctgcttgttt gaaaaagaac atcatcttcg cattgcgact
      301 attctccaag cccttttatg gaaactcatt cgtgaccttg ttccggcgaa agcaaatagg
      361 taaatctgat tttttgaact aacgaactcg ttaaccatgg gggctctaat gagttttaac
      421 tttcgtccaa atactctcat acatctgatt aaccttaccc aggtcatgaa tttgctcgag
      481 cttcttgaat tgttcatccg atggaaacag cgccttgtta ttttttaaat cctcaggcaa
      541 caaatcacga gtcgtagaaa gaactggacc gcaacgaacc tttttaataa agactaaatt
      601 attctcagtt ttaagcatat agtcgattaa cttcagagca ttgtctttgt gctttgcccc
      661 tttcacaatc accatattat caatggatcg agtgccgcca tcattgagaa gaataaactc
      721 aatctctcca ttgctttggg cctgagcttg cagggcatcc gaagaaaaag aatgagccac
      781 cgccacttct ttattcttta gaatctctat cgtatcagaa gtgaaagtct ttacgttcgg
      841 ttttactttc aacaatgtct cttcagcttc attcaattct tttacatcgg tgctattaat
      901 actgtggcca tgcagtctta aggcggtacc gatgacttca cggccatcat ccaaaagcga
      961 aaatttacct ttcaactctg gattttcaaa aatgtccttc caacttttga tacttccctt
     1021 gtagagactg cggttcacag cgactcctgt ggtggtccag gcatagggaa tggaaaacgc
     1081 attcgttggg tcataggact gggctaaaaa ctttggcgac aaatttttaa aattggaaat
     1141 ttgagttttg tccaccggct ctaaaagatt gagcttaatc atgatatcaa ccatgtaatc
     1201 cgaaggcacg gccatatcaa ttcccgaagc acccatctga attttggcca ggagttcttc
     1261 gttggaggaa taattagtga tattaatttt aatgccggtt tcgtcagtaa atttcttttg
     1321 cacttctgcg gataagtaat taccccaaat ggctaggttg atttccgaag aaggcttcgc
     1381 ttcctgcttt ttagtgcagg aaagcaagct aagagaaata agaaaaatga taagtgaacg
     1441 catgcaggaa ttaaacaata tctcaagtcc tttagcaaca gtttgatttc tataagcgtt
     1501 acgataagct taaggaaatg gcgaatttca aaatcgattt cttcgttcac cccattgctt
     1561 tatttcccgt agctttgatg gcagttaatg atcattggct gaagtacgct tatcccggct
     1621 ggttcacggg aaagctcagt gactttgcgg gaatttttta ttttcctatt ttcatcttgg
     1681 ccatggtcat ttttgtattt catgtggtgt tgacgagacg aaacctctgg ctcgcgatct
     1741 tgttcacgga ctttctattg atgttggtca aattaaatga acctgcggca cgctggattg
     1801 aaacgatctt cgctcaatac ttatttagga tccaactaat tcaagatcct acggacttgc
     1861 tcgctcttag tatgaatgcg ctgacttata tttatatgaa gaagtatctt actggcgctg
     1921 gagaacctgc tcaaagctca cttgacaaat aggtctattg ttttcgtagc agttgtagac
     1981 atggagaagg cctgtgtcgc tatcagcttc catcacggaa aggtagcaca acatttgata
     2041 gccacgcacc catcctttca cttgcggata catttcaaaa tttaaacgcg tcaagggcaa
     2101 gccaaaatcg gcaaactgca gatttgtggg atatgaaatg gtttcattgg tcactgtaat
     2161 ttgatcttga ggaagggctg cggggcagtt ggaggccaca taatattgct tcgattccgc
     2221 tgtgatcaca tcatctgatc ctgttttagg aggaggagcc tttgacgtgg caaccgtaac
     2281 caatgaggtg gtcaaaaggc acaatacagt aaaccatgat ttagcagtcg tattcatgaa
     2341 aacctcgcaa aaatgggccc tccgatgacc aatctttaga cagcaattcg ctccagatca
     2401 acacacgtta agctgtggct ttgttataaa gctctgtttt aaattattca aatcgtatgc
     2461 caagtatcca caatcttaag caccactatg ccgtactatt tactgaaact tcctcttgct
     2521 tcagtttaca tataatatac aatatattac atattatcag caaaattagc caataaagga
     2581 caatattttg gactttaaat tgtcgacagc ccaagagatc tgttacgaat tgggacaacg
     2641 attgaagact cagcgtctgg ccaagaatgt aaaacaacaa gagcttgctg agcaggcggg
     2701 tatctccgtg ggaactgtta aaaatcttga gtccaagggg caggcttctt tagaaaactg
     2761 gattcgaatc atcatcgcct tggatctaac ccaagatctg gaagctcttt ttactctaaa
     2821 aattcgttcc atttcagaaa tggaaaaaat ggaaaagctc aaatcaaata agatttcccg
     2881 gagggctcga tgaaaaaggt agatgtcttt tactctggct ggggcgagca ttggaaatta
     2941 ggcagactgg ccgacaacgg aacgaaactt cttttcgaat ataccgcaga agcccttgag
     3001 caaggattag aactttcacc gctcaatctt aaattgaaaa gtgaggccta tggagagttt
     3061 cctactcatc aacagcgcct tccaggattg atagcggaca gcctgccgga tggctggggc
     3121 ctgctattga tggataaatt atttcgaaaa caagggcttg ccattgagga ggtgtctcct
     3181 ttggatcgac tctcattcct cggagatcga gctatggggg cgttgatttt taaacctgcc
     3241 tcggagtcag tggctcagcc aaaaggctta agtttactcg aaatcggcac tgaagttcac
     3301 aaaatcgtct ccgaaaaagc cagcagtgca ctttcagagc tggcaatcct aggaggctca
     3361 ccgcaaggag ctcgccccaa agttctggct tattatcaga aaaaaacaaa gcagctttca
     3421 atgacgtcgg ttccaacggc gcatccctgg ctttttaagt ttccggctca acaagagcat
     3481 aaggaagttt gtgcgattga ggccctctat gcgaaattag ctaagaaagc tggtcttgaa
     3541 atcaaagaga ccgaatattt tgaactcagc aaaaaccttt cggcctttgg cacaaaaagg
     3601 tttgatcgtg aaaatgacct acgcatcccc acccatactc tggcgggtgc tttgaacgct
     3661 gatttcagac gttccttctc tgtcgattat ctctcgttgc tgcgtctcac tcgattgatg
     3721 accaaagatg aacgtgaggt tcacaaagca tttcgccagt gtctctttaa tgtgattttc
     3781 aataacagag atgaccatgc aaagaacttt tcttttctac tggacgacaa aagaagatgg
     3841 aaactcagcc ctgcctacga cttgaccttc aatgccggac ctggtggaga acaccaaatg
     3901 gatatttgcg gagaaggccg cgcacctact aagactcatc ttttgaagct ggcagaaaaa
     3961 tctggaattc acatcaaagt ttttgaggaa atcctggaag aagtcactgc ggcggctcga
     4021 caattcaaaa cggaagccaa gaattggcca cttcggaccg caacaatcca caccatccac
     4081 caaacaattc aagccaacag taaacggcta aaattgtaag cttaaggtaa agatctgaat
     4141 aagtctggtc gagggaggat ccttacccca atggccaggt tgatttcaga aagggttttt
     4201 cgattcctgc tttttagtgc atgatgatga acacatgtta gagttgagcc acatatcaaa
     4261 aaccttcagc aatcaaatgg ccttacgaga aattgggcta aaaattcgta aaggcgagtt
     4321 cttttccttg ttaggtccca gtggctgcgg caagaccact ttgctgcgca tcctggcggg
     4381 gtttgaaact cccactcagg gtcgcatctt gttggagcaa aaagatattt ccgggcttct
     4441 gcctccggca cgtcctttta atatggtctt tcagaaatac gcgctctttc ctcacttaac
     4501 cgtgcatgaa aatgtggctt tcggtctgaa aatgaagaag ctcccgaacg aagagattaa
     4561 gcgacgagtc gacgaaatgc tgcaaatggt aggattgaag gagtatgctc agcgcaagcc
     4621 cgaaactctg tcgggtggtc aaagtcaacg ggtcgccgtg gcccggtccc tggtaaatca
     4681 gccaaaagtg cttttgctgg acgagccctt gtccgcgctg gatttgaaaa tgcgcgaaca
     4741 tatgcaaatc gagctgaaaa ctctgcaaag aaaattagga ctgacattca tctttgtcac
     4801 tcacgatcaa gaagaggcct tcgctctgtc agatcgcata gcggtgatga ataaaggcgg
     4861 cattgagcaa gtcagtactc ctcaagaact ctatgaacag ccacagacac gctttgttgc
     4921 tgaatttata ggtcatatga ataaacttcc tgctcactta ctgcctccgc acttaatcac
     4981 tgcggccaaa gattatttta tccgaagtga gaaaatcttt ttcaaaggac actcgtccgc
     5041 accgatgact gtcaaagcac aggtattaca gaaaaccttt cgcggtgatc acgacgaatg
     5101 gcaggtcgaa gtttcagaca aaactctcct gaaactaaaa acgccggtag gagcgcacac
     5161 attgtccgca ggtcaaccgg tggaaatttg tttcgatgaa aaggatgttt ttagttttaa
     5221 ggatgaagtc tgatgaaggt gcgtgaactc actgggccgg ccctcttttg gtacggactt
     5281 tttttgatgg gtcctctcct ttggattttt attttgagtt ttcaaacccg tggaacttat
     5341 gggggcattg tttggacacc gaccttggcg aactatgcca aggtcttgga gcctgcttat
     5401 tttttaatat acctcaagag cctgatcttg gctttcagca cggcggctgg aactgttttt
     5461 ctcgcggtta ttttgtcctg ggcggtcgtc agcgtcgata aaaaatggcg cattctttat
     5521 ctagcagcac tggttttacc ttttctaatc aatctcatta ttcgagttta cgcccttcgc
     5581 attcttctca gttacgatgg ccccttgcag atgatgctca gagcttttaa cattgagttt
     5641 gatccttttt ccctgaccag caataacggt ttggtttttt atgggatgat caccacttat
     5701 ttgccatttg cggtattgcc tatttactca gccctcagtc gatttgattt caccctcttt
     5761 gaagcggcgg tggatttagg tgcgaccgcc agccagacct ttttaaaagt ggttgccccc
     5821 tctttaagag gagcgatgac cagcgccttt gccctggtct ttatccccgc cctcggagaa
     5881 tacatcatcc cggacttact gggaggggca aaaaccatgt acgtgggaaa cttactgacc
     5941 gaagaattct taaaatcgcg ggattggcct cttggctcgg ctttagcgat gactttaatc
     6001 atggtgcttt tgttaatgct att
//
LOCUS       DATBTE010000392         9316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2240567, whole genome shotgun sequence.
ACCESSION   DATBTE010000392 DATBTE010000000
VERSION     DATBTE010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 9316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9316
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2240567"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..553
                     /locus_tag="VIG33_15000"
     CDS             203..553
                     /locus_tag="VIG33_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272196.1"
                     /translation="MKNLIFLSLMTIVSATNAQAATQSIYVEGHSPVGSFISSLRGQS
                     ATIEGLLIQKAKEICASDSSFSISNLQVSISSRGGRLNLNGERDTLDHQNLALSLGQP
                     HSTYSAYIHCDQPF"
     gene            complement(599..2521)
                     /locus_tag="VIG33_15005"
     CDS             complement(599..2521)
                     /locus_tag="VIG33_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEY8272197.1"
                     /translation="MTEFVRRFEKLLSLAALSFVFYFFARIEFLAWNWSHFKSKEISD
                     LLWAFFVGFRFDISAVCMSLIPVTLWSFWPWSKKRHRIWGLGAFWLYAPFFSAFMIMN
                     LGDTEFINFVGRRFSYDALFVLGELNNKLGSILGSYWLLALIDVGTVSLFLWGVWKII
                     QRPSKRVYLAPKKYEIPSYATAVFFVLVLVVVGVRGGLQKKPLNIVNAHLFVAPILNN
                     LVLNSTFTFIKSYGAEALSHAVYFSDRNEMLQHLNGGIKGPSLLEGHRLKKAQNVVVI
                     ILESFSLEYMGEVNGGQGFTPFLDSLAHKSLFFKNSYANSRRSIEGVAAVVAGVPAMM
                     NEPFISSHFSSNYFVGLGSLLAGQKYSTGFFHGGNNGTMYFDSFAKSAGYDYYYGANE
                     FPDPSQNDGTWGIFDEPFLQFMRMKIHSMKPPFLASVFTLSSHNPYRIPEQYKGKFPK
                     GPLEILESVAYADYSLQRFFEEAAKEPWYKDTLFVITADHTGLNYRPENENELSRFRI
                     PILFYHPSYQWPAFIQQDQVVQQIDILPSIMDFLGLQNKEMNYLGRSVFIPGERTATM
                     YLDGRYFLVANDFFLDGLMNGTEMKMYALQDSFEKEPLAEPKERRAHLEERLKASIQY
                     FNEGMWDNRLYYPSGK"
     gene            2610..2837
                     /locus_tag="VIG33_15010"
     CDS             2610..2837
                     /locus_tag="VIG33_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEY8272198.1"
                     /translation="MTPQEMKDRLTSAYPDGKIEVYDTTGTQDHWEVSVESSKFAGLS
                     RIQQHQQVMAVFGPELKTGEVHALSIKTKVK"
     gene            2864..3172
                     /gene="grxD"
                     /locus_tag="VIG33_15015"
     CDS             2864..3172
                     /gene="grxD"
                     /locus_tag="VIG33_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEY8272199.1"
                     /translation="MSTTHERIEKLLNENKVVLFMKGTQSFPMCGFSSRACAILQDMG
                     VQFHDVNILEDEELRQGIKEYGNWPTIPQLYINKQLIGGSDIMMEMYENGELQELMKS
                     "
     gene            3178..3402
                     /locus_tag="VIG33_15020"
     CDS             3178..3402
                     /locus_tag="VIG33_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEY8272200.1"
                     /translation="MKCNIALWDRILRFIIGVGFFTYAIAGGPIWAFGGIILIFTSAW
                     GFCPLYGTLKIRTLREEKRAPHINPFEEQS"
     gene            3399..4787
                     /locus_tag="VIG33_15025"
     CDS             3399..4787
                     /locus_tag="VIG33_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY8272201.1"
                     /translation="MMSPLPELSHLLPQNQIKTDTDSLKYYGKDWTTYFDINTSAVLF
                     PHTTEEVAKIVHWARKYKIALVPSGGRTGLSGAAVASKGEVVISFEQMNQILTFNSTD
                     QTVVCQAGVVTEALQEFAQSKNLCYPVDFAASGSSQIGGNIATNAGGIKVLRYGLTRD
                     WVAGLKVVTGAGEILELNNALVKNATGLDLRHLFIGSEGILGFVTECTMRLAPPPPPL
                     KVMVLGVSSLEAVMKIFAQFKNKTTVVAFEMFSEKALKCVIENTGLSRPFETETPFYV
                     LAEVEARNEQDEEQSLNVFEKCLLEGWVSDGVVSQSEAQAKNFWRLREDISESLAKYS
                     PYKNDISVTISQVPAFMAELESILAKAYPTWEVVWFGHLGDGNLHVNILRPEGMTKED
                     FVKECSKVDVLVFEAVMKHKGSISAEHGVGLTKKPFLRFTRSQAEVEMMKGIKRVFDP
                     DNIMNPGKVIDL"
     gene            complement(4816..5400)
                     /locus_tag="VIG33_15030"
     CDS             complement(4816..5400)
                     /locus_tag="VIG33_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEY8272202.1"
                     /translation="MVMSKKLSGKKIAILSTDGFEQSELFEPRKALIEAGAEVHIISL
                     KAGQIKGWSDGNWGSSIKVDELASEADAEEFDALMLPGGVINPDRLRIDENAIEFVES
                     FLRAGKPVAAICHGLQTLIETGLIAGKTMTSWRSLKTDLLNAGATWMDQDVVVDQGLI
                     TSRKPEDIPAFNRKMIEEFAKGIPEIHPAQPPIH"
     gene            complement(5519..6745)
                     /gene="serA"
                     /locus_tag="VIG33_15035"
     CDS             complement(5519..6745)
                     /gene="serA"
                     /locus_tag="VIG33_15035"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020797315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEY8272203.1"
                     /translation="MGVYAMKSNSLKILLVENIHQCAKEKLEEEGFKVDLISYAPSEE
                     ELLKTLPNYQALGIRSKTELTTKVFEKNPHLFVVGAFCIGTNQMDLKAARQEGIAVFN
                     APHSNTRSVAELVIAEMIALSRQLGDRNTLAHKGEWMKSAEGSREVRSKTLGIVGYGH
                     IGSQVSILAENLGMKVLFYDVLKKLPLGNAQAKSSLEDLLKACDFVSLHVPETPQTKN
                     MIAAKQLKQMKKGSHLINASRGTVVAIEDLVEALQVKHLAGCAIDVFPEEPASNKEKF
                     LSPLQGIGNVILTPHIGGSTEEAQYSIGMEVAESFRRYLKIGSSSGAVNFPNVDLPVK
                     QGTSRILNVHRNEPGVLGEINGIISKEGANIEAQYLSTDDKVGYMVMDLNSHHAQQLA
                     DEIQKLGRSIRTRVVY"
     gene            6854..8218
                     /locus_tag="VIG33_15040"
     CDS             6854..8218
                     /locus_tag="VIG33_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8272204.1"
                     /translation="MNVNRAFHLLVIDDDTLIHQSLKLCVGTPWKITSVSKKELIPFN
                     NFYHAAMVDMHLTSGSKDPEGVLVIEKLMKYNPQLEIVAMSGDLNRETMELCLKAGAQ
                     RFLAKPLMPEEVLLVLNKIEAYWSIRNIDSVGKNISWIGTGPASQKIKKCIANAKGEK
                     KTVLIEGETGCGKEVVARLLNQQESERPFVTVNISGIPENLFESELFGHTKGAFTGAD
                     QNKIGLTEAAHGGDLFLDEIEALPLSQQVKLLRFLESGEVRRVGAKESFYVQTRVIAA
                     SNQPLKKLIAEGKFREDLYFRLCGQPLKLPPLRERKEDIRELAKNFLEQEKPQRNKQF
                     TEDGFEALHHYPWPGNVRELKRVCEQLSLTCPLPFIRREDVENLLHVENQKKTSLSST
                     AIAEDMDFTKGLNDLVAEFEADLIRACLRKYKDVEEASKVFKISRSNLYKKIKDYQID
                     EAAL"
     gene            8215..9135
                     /locus_tag="VIG33_15045"
     CDS             8215..9135
                     /locus_tag="VIG33_15045"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY8272205.1"
                     /translation="MMGTSWSSPVYQHTVLIVLSIIFGSGLIVYLFRKSNYYFIASWA
                     SIKSWLIVAPLLFILMGLPSPLPLMVLVLIAILGAKAYFQIMGMYNRSYFVLLCYAGI
                     LALGACAYFNRVDIYNVMPMAVLGAACLIPMARNNYKHMIQYMSLTLLGFIFLGWSFM
                     HLGLILHLENGIYQLIYLIILTEFCDNTNLAVGRYFGKIKIFPKLNQRRTLGGLSVSI
                     ILTLVLAGVMRSLLPDRSDKYWLTAGLIASLGGFIGDLVMSVIRKDAGVKIVGPFIIG
                     RGDFLHRMDRLIFVAPIYFYIMLALPDISL"
     gene            9132..>9316
                     /locus_tag="VIG33_15050"
     CDS             9132..>9316
                     /locus_tag="VIG33_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEY8272206.1"
                     /translation="MKVWNYDNEQWTKLPTYLKHLPLFTRHIDLFSTVMRYVWSIFLK
                     DVVFRFYIRLKVTGGSFP"
BASE COUNT         2553 a         2150 c         2071 g         2542 t
ORIGIN      
        1 gcctcaacaa agtttttaac atcggtcatt ctaatgttca tcgagtatct acctacctta
       61 ataatcacaa tattgtatag caatttagtt cataggcatt tacctaatga aggtataggt
      121 aaataccaag tttacctata aaataagtaa gcttatgttt tccccgaagc attatgcttt
      181 aagtaaacaa tagggagatt ttatgaaaaa cttaattttt ttatcgttaa tgaccattgt
      241 cagtgcaaca aatgcccaag cagctactca aagtatttat gttgaaggac acagtcctgt
      301 gggctctttt atttcttcat tgagaggaca gtccgctacg atcgaaggac tgttaattca
      361 aaaagctaaa gagatttgcg cgagtgattc ttcgttctct atttcaaatc tccaagtttc
      421 aatttcttct aggggaggtc gtctaaattt gaatggcgag cgcgacaccc tagatcatca
      481 gaatctggct ctttctcttg gccaacctca ttccacttac tccgcctata tccattgtga
      541 tcagcctttt tagccttatt ttttaaaagc cttagtttaa cttaaattta agttaaactt
      601 acttgccact gggataataa aggcgattat cccacatgcc ctcattgaaa tattggatag
      661 aggctttaag acgctcttcg agatgggctc gccgctcttt tggctcagct aagggctctt
      721 tctcaaaact gtcctggagg gcatacattt tcatttctgt tccattcata aggccgtcta
      781 agaaaaaatc attcgccact aaaaaatatc tgccgtccag atacatcgtg gcggttcgct
      841 ctccgggaat aaaaacagaa cgtcccaagt agttcatttc cttattttgc agacctagga
      901 agtccatgat ggaaggtaaa atatcaattt gctgcaccac ctgatcttgc tggatgaatg
      961 caggccattg ataactcgga tgataaaata aaatagggat gcgaaaccgg gaaagttcat
     1021 tttcattttc cggccggtaa ttaagacccg tatgatccgc cgtaatcacg aaaagagtgt
     1081 ctttgtacca aggctcttta gccgcctctt caaaaaatct ttgcagtgaa taatccgcat
     1141 aggcaacgct ttcgaggatt tccagaggac cctttgggaa tttccctttg tattgttcag
     1201 gaatccggta aggattgtgc gaactcaacg tgaatacgga ggcgaggaag ggcggcttca
     1261 tggagtggat cttcattctc atgaattgca aaaaaggttc gtcgaaaatc ccccacgtgc
     1321 catcattttg gctggggtcc ggaaactcgt tcgccccata gtaataatca taacccgcgc
     1381 tcttagcgaa ggaatcaaaa tacatcgtgc cgttgttacc gccatggaaa aagccggtgg
     1441 aatatttttg acctgccaac agactgccca accccacaaa atagttggag gaaaaatgag
     1501 acgagataaa aggttcattc atcatcgccg gcaccccagc gacgaccgct gcgacgccct
     1561 caatggatct cctggaattc gcgtaactgt ttttgaaaaa gagactttta tgcgctaggg
     1621 aatccaagaa gggagtaaag ccctggcctc cgttgacttc gcccatatac tcaaggctga
     1681 agctctcaag aatgatcacg accacatttt gcgctttttt caaacgatga ccttcgagaa
     1741 gagaaggccc tttaattcca ccatttaagt gctgcaacat ttcattgcgg tcggaaaaat
     1801 acacggcgtg agaaagggct tccgctccat aacttttgat aaaggtaaaa gtggaattca
     1861 gcacgagatt gttaaggata ggagccacga aaagatgggc gttcacgatg ttgagaggtt
     1921 ttttttgcag ccctccgcgc acccccacca ctacaaggac aagaacgaaa aagacggccg
     1981 tggcgtagga cggaatttca tatttcttag gggctaaata aacccgcttt gagggacgct
     2041 gaataatttt ccaaacaccc caaagaaaaa ggctcaccgt ccccacgtca atcagtgcca
     2101 aaagccagta ggaacccaaa atgctgccca acttgttatt caattcaccc agcacgaaaa
     2161 gagcatcgta ggaaaaacga cgacctacaa aattaatgaa ttccgtatcc cccaaattca
     2221 tgatcatgaa ggctgaaaaa aacggcgcat aaagccaaaa ggcgcccagg ccccagatgc
     2281 ggtggcgttt tttggaccag ggccaaaagc tccagagagt cacggggatt aagctcatac
     2341 aaacggcgga aatatcaaaa cgaaagccca caaaaaaagc ccaaagcagg tcggaaatct
     2401 ctttggattt aaaatgggac caattccatg ccaggaactc gatccttgcg aagaagtaaa
     2461 agacaaagga cagagccgca agactcaaaa gcttttcgaa acgacgaaca aattcggtca
     2521 tttcgggatc ttacctgctg agagaggcca ctgtcacctg ttcagttagc acttgctccg
     2581 aaactgctct tctggtagct ttttcctcaa tgacaccaca agaaatgaaa gaccgtttga
     2641 ccagcgctta ccctgacgga aaaattgaag tctatgacac cactggcacc caggatcact
     2701 gggaagtcag tgtcgagagt tcaaagtttg caggccttag ccgcattcaa caacatcaac
     2761 aggtgatggc cgtctttgga ccagaactta aaactggcga agttcatgct ctttccatta
     2821 aaacaaaagt aaaataatta tataaataat gccggaggac tttatgtcta ctacccacga
     2881 acgtatcgaa aaacttttga atgaaaacaa agtcgtgctt tttatgaaag gcacccaaag
     2941 cttccccatg tgtggattct cttcccgcgc ctgtgcgatt cttcaggata tgggagttca
     3001 atttcatgac gtgaatattc ttgaagacga agaactccgc caaggaatca aggaatatgg
     3061 caattggccc accattcctc agctctatat taacaagcaa cttattggcg gaagcgatat
     3121 catgatggaa atgtatgaga atggcgaact tcaagaactg atgaaaagtt aaaacttatg
     3181 aaatgcaaca ttgccctctg ggaccgcatt ctgagattta tcattggcgt cggttttttc
     3241 acctacgcta ttgcgggcgg tccgatctgg gcatttggcg gaatcatttt aatttttact
     3301 tcagcctggg gcttttgccc attgtatgga actctcaaaa tccgcacgct gcgcgaagaa
     3361 aaaagagctc cccacatcaa cccttttgag gagcaatcat gatgagcccg cttccggaat
     3421 tatcccacct tcttcctcaa aatcaaatta agaccgatac ggattccttg aaatactacg
     3481 gaaaagattg gaccacttac ttcgatatca atacttcggc cgtccttttc ccccacacca
     3541 cggaagaagt ggccaagatt gtccactggg ctcgcaaata taaaattgcc ctggtccctt
     3601 ctggcggtcg cacgggcctc agtggcgcgg ccgttgccag caaaggagaa gtggtgatct
     3661 cttttgaaca gatgaaccag attctgactt tcaactccac ggatcagaca gtggtttgcc
     3721 aggcgggagt cgttactgag gctttgcaag aatttgctca gtctaagaat ctttgttatc
     3781 cagtggattt tgcggccagc ggttcttcgc aaattggcgg caatatcgcc accaacgccg
     3841 gaggcattaa agttttgcgc tatggcctga cccgtgattg ggtggccgga cttaaagtgg
     3901 tcaccggcgc tggcgaaatc ctggaactga acaacgcttt agtgaaaaac gccacgggct
     3961 tagacctgcg ccatttattt attggcagcg aaggcatcct gggttttgtt actgaatgca
     4021 cgatgcgctt agccccgcca ccaccgccct taaaggtgat ggtgcttgga gtttccagcc
     4081 ttgaagcggt gatgaagatc tttgcgcagt ttaagaataa aaccacagtg gtggcctttg
     4141 aaatgttctc tgaaaaagct ttaaaatgcg tgatagagaa tacgggactt tcccgtccct
     4201 ttgaaacaga aaccccgttt tatgttttag cggaagtgga agctcgcaat gagcaggacg
     4261 aagagcagtc tctgaatgtg tttgaaaagt gcttgctgga gggctgggtt tcggacgggg
     4321 tggtgagcca atcagaggct caagcgaaga atttttggcg attgcgtgaa gatatcagcg
     4381 agtctttggc aaaatactct ccttacaaaa acgacatctc cgtgacgatt tctcaagtgc
     4441 cagcgtttat ggcggagctt gaatccattc tggcgaaggc ctatccaacc tgggaagtgg
     4501 tctggtttgg ccacttgggg gatggaaatt tgcatgttaa tattttgcgc ccggaaggca
     4561 tgaccaagga agattttgta aaagaatgca gcaaggtcga cgttctggtc tttgaagccg
     4621 tcatgaaaca caaaggctcc atctccgcag agcatggcgt gggcctcacc aaaaagccgt
     4681 tcttgcgctt cacccgttct caggccgagg ttgaaatgat gaaagggatt aaaagagttt
     4741 ttgatcctga caatatcatg aatccgggta aagtgattga tctgtaattt ttttctttaa
     4801 tcctcttctt agacattaat gaatcggagg ttgggcagga tgaatctcag gaatcccttt
     4861 ggcaaactcc tcgatcattt ttctattaaa ggccggtata tcctctggct tacgactggt
     4921 aattaatccc tgatctacga cgacatcttg atccatccag gtcgcaccag cattgagtaa
     4981 atctgttttc aatgagcgcc aggacgtcat ggttttaccc gcgatcagtc ccgtttcaat
     5041 caaggtttga aggccgtggc agatggcggc cacaggcttt cccgcgcgta aaaaactttc
     5101 gacaaattca atcgcatttt catcgatacg gagccgatcc ggattgatca ctccccccgg
     5161 gagcatcaac gcatcaaatt cctcggcatc cgcttcactc gcgagttcat cgactttaat
     5221 gctgcttccc caattgccgt cgctccatcc tttaatctgt ccggccttca aagagatgat
     5281 gtgaacttcc gcgccggctt cgatgagggc tttgcggggt tcaaaaagct ccgactgttc
     5341 aaaaccgtcc gttgaaagta tcgcaatttt tttaccactg agctttttag acatcaccat
     5401 aaagaatccc ctcgtttaaa tgaaccaaaa cttatcttaa aggcgactta tgacgagggt
     5461 gtgaatttcc tgtgattttc ttaatcaaat aatctgatga aaccccagaa gtcgctatct
     5521 agtagaccac acgagtgcgg atactgcgcc cgagcttttg gatttcatcg gccaattgct
     5581 gcgcgtgatg cgaattcaaa tccatcacca tgtacccgac tttgtcatcc gtggaaagat
     5641 actgagcctc aatgttggcc ccttccttcg agatgattcc gttgatttcc ccgagaactc
     5701 ccggttcatt gcgatgaacg ttcaagattc tggaagttcc ttgcttgacc ggcagatcca
     5761 cgttcggaaa attcacagcg ccactgctgg aaccgatttt cagataacgg cgaaaacttt
     5821 cagcgacctc catgccaata gagtattggg cttcttccgt actgccgccg atatgcggag
     5881 tcaaaatcac attgccaatc ccctgcaaag gcgaaagaaa tttttctttg ttcgaagccg
     5941 gctcctcagg gaaaacgtca atggcgcaac ctgcaagatg tttgacttgc aaagcctcca
     6001 ccagatcttc gatcgccacc actgtgccgc ggctggcatt gatcaaatgg ctgccttttt
     6061 tcatctgctt taactgcttg gctgcgatca tatttttggt ttgtggagtc tccgggacat
     6121 gcaggctgac aaagtcacag gccttcaata aatcctccaa tgaagatttc gcttgagcat
     6181 tccccagagg gagtttttta agcacgtcat aaaagagcac cttcatccca agattttcag
     6241 ccaaaatgct gacttggcta ccaatgtgac cataccccac gatgcccaag gttttactgc
     6301 gaacttctcg agagccctcg gcgctcttca tccactcccc tttatgagcc agggtgttgc
     6361 gatcccccaa ctgacgggac aaggcgatca tttccgcaat caccaactca gccacactgc
     6421 gagtgttcga gtgaggagca ttgaaaaccg caatcccctc ttggcgagcc gctttaagat
     6481 ccatttgatt ggttccgata caaaaagcac cgaccacaaa aaggtgaggg tttttctcaa
     6541 agactttggt ggtgagttct gttttagagc gaatgcccaa agcctgataa tttggaagag
     6601 ttttcaatag ttcttcctcg gaaggagcat aggagatcaa atcaactttg aacccctcct
     6661 cttctaattt ttctttggca cattggtgaa tattttcaac taatagaatt ttcaacgaat
     6721 tggatttcat agcgtaaact cccacgcaaa aagtttaacc tctttcaagc aattttacca
     6781 agccttgagg ttctcgcttt aacgtcatgc accaagggta gccaaagtaa ctcgctaaag
     6841 ggatgctttc ataatgaacg ttaatagagc ctttcatctg ctggtcattg atgacgacac
     6901 tttaattcac caatccctaa aactatgtgt gggaactccg tggaagatca cttccgtctc
     6961 taaaaaagaa ctgatccctt ttaataattt ttaccacgcg gccatggtgg atatgcatct
     7021 gacttcagga agtaaagatc ctgaaggcgt cctggtgatt gaaaaattaa tgaaatacaa
     7081 tccgcaactg gaaatcgtcg ccatgtctgg agatctcaat cgcgagacca tggagctttg
     7141 tttaaaagcg ggagctcaga gatttcttgc taaacctctg atgccggaag aagtgctttt
     7201 ggttttgaat aaaatcgaag cttattggag tattcgcaat attgactcag ttggaaaaaa
     7261 tatttcttgg atcggcactg ggccggcttc acaaaaaatt aaaaagtgta tagccaatgc
     7321 caaaggcgaa aaaaaaacag ttctgattga aggtgaaacc ggttgcggca aagaggtcgt
     7381 ggcgcgctta cttaatcagc aagaaagcga acgccctttt gtgaccgtga acatttccgg
     7441 tattccggaa aatctttttg aatctgaact ctttggccat accaaagggg cttttaccgg
     7501 cgctgatcaa aataaaatag gtcttactga ggcagcccat ggtggagatt tgttcttgga
     7561 tgagattgag gccttgcccc tttcccagca agtcaaactt ttgcgatttc tcgagtccgg
     7621 ggaagtccgc cgggtcggag ccaaagaatc tttctatgtt caaacccggg tcattgccgc
     7681 tagcaatcaa ccgctcaaga aactgattgc tgaaggcaaa ttccgcgagg atctttactt
     7741 tcgactttgt ggccagcccc ttaagctgcc acctttaaga gaacgtaaag aagatatcag
     7801 ggagctagcg aagaatttct tggaacaaga aaaaccacag cgaaataaac aattcactga
     7861 agatggcttt gaggctttac atcattatcc atggcccggc aatgtgcgcg aattaaagcg
     7921 cgtctgtgaa cagcttagcc tgacctgtcc gctgcctttt attcgccgtg aagacgtgga
     7981 aaatttatta catgtagaga atcaaaaaaa gacctctctt agctccacgg cgatcgctga
     8041 agacatggat ttcaccaaag gcttgaatga tttagtggca gagtttgaag ccgatctcat
     8101 tcgcgcctgt ttacgaaaat ataaagatgt agaggaagcc tcaaaggttt tcaaaatatc
     8161 acgttcaaat ttatataaaa aaataaaaga ctaccaaatc gacgaggccg cattatgatg
     8221 ggtacttcct ggtccagtcc cgtgtatcaa cacacggtac tgattgttct tagcattatt
     8281 tttggctcag gcctgattgt ttatctcttc cgtaagagca actattattt tattgcaagc
     8341 tgggccagca ttaaaagctg gctgattgtg gcgcctcttc tttttatctt gatgggttta
     8401 cccagccctt tgcctctgat ggtgctggta ctcattgcga ttctgggcgc gaaggcctat
     8461 ttccaaatta tgggaatgta caatcgaagt tattttgttt tgctgtgcta tgctggcatt
     8521 cttgcgctgg gtgcctgcgc ttatttcaat agagtggata tttataatgt catgcccatg
     8581 gcggtcctag gggcggcctg cttgattccc atggctcgca ataattataa acacatgatc
     8641 caatacatgt cgctcactct cctcggattt atcttcctag gttggtcctt catgcatttg
     8701 ggcctgattc tgcatctcga aaatgggatc tatcaattga tttacctcat cattttgact
     8761 gaattctgcg ataataccaa tttggcggtg ggtcgttatt tcgggaaaat caaaatcttt
     8821 ccaaaattga atcaaagaag aactctcgga ggactctcgg tctctatcat actgaccttg
     8881 gttcttgcgg gtgtcatgag atccttactg cccgatcgat cagataaata ctggttgact
     8941 gcggggttga tcgccagtct aggcggcttt atcggcgacc tcgttatgag cgtgatccgt
     9001 aaggatgcgg gagtaaaaat tgtagggcct ttcattattg gacgcggtga ttttctgcat
     9061 cgcatggatc gattgatctt tgtcgctccg atttatttct atattatgtt agctttgccg
     9121 gatatttcct tatgaaggtt tggaattatg ataacgaaca gtggaccaag ctccctactt
     9181 acctaaagca tcttccgctg ttcactcgcc acattgattt atttagcact gtcatgcgct
     9241 atgtgtggtc tatttttctg aaggacgtgg tctttcgatt ttatatccgt ctgaaggtca
     9301 ctggcggaag cttccc
//
LOCUS       DATBTE010000393        12233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_806632, whole genome shotgun sequence.
ACCESSION   DATBTE010000393 DATBTE010000000
VERSION     DATBTE010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 12233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12233
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806632"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VIG33_15055"
     CDS             complement(<1..712)
                     /locus_tag="VIG33_15055"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEY8272207.1"
                     /translation="MPRSGLKNIFFLFTIFVTLAIITLAAGISYAFLSLSEEMDQKLE
                     AKRILLPTEYYAAPYRFYTPSLQDVDKSEKALQGQNYRSRDFQQRLLPGDYLKASTLE
                     CKTLFRIPEEINFDNCIAFIKKDVDPGQVQNNIHLLLFNNENRIQQIFKTFPLEPLSE
                     VSLEAPLVAQYVGIDPIMQEEQPLGKVPPFCSNGVMAIEDPRFLEHKGVNYRGIFRAL
                     VKNITSGRAVQGGSTITQQ"
     gene            721..999
                     /locus_tag="VIG33_15060"
     CDS             721..999
                     /locus_tag="VIG33_15060"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HEY8272208.1"
                     /translation="MKLSSQQIQVLAEKILNHWKKQNLIVFKEDEKVVLARMVELIKE
                     DYAREAALDQEVNAMLDQLEKSNPGEFQRFKMFPILKQKLAKERKVIL"
     gene            996..1271
                     /locus_tag="VIG33_15065"
     CDS             996..1271
                     /locus_tag="VIG33_15065"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HEY8272209.1"
                     /translation="MIISGDRQNHLAHIVTDGVWGDDIADFMDDDQALRVAKLAIAEF
                     VKEDEDLDQRARDKVASLKRNVMEGTPEWDIMYKKYYQEERVKRGQK"
     gene            1258..1719
                     /locus_tag="VIG33_15070"
     CDS             1258..1719
                     /locus_tag="VIG33_15070"
                     /inference="COORDINATES: protein motif:HMM:NF012393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY8272210.1"
                     /translation="MAKSKKTVAKKSAKKPAKKASAAKKTVVKKTAAKKAAKGKAAPK
                     KVVNKKTKIDLSRVVTPLDDRVLVQLVEKETMTAGGLYIPDTVSDVSGNLEGFVISVG
                     RGHRNKKGYVRPMDLKVGDKVLFSEYAGSKIKIQDQDLLIVREIDVLGVIA"
     gene            1740..3152
                     /locus_tag="VIG33_15075"
     CDS             1740..3152
                     /locus_tag="VIG33_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HEY8272211.1"
                     /translation="MNLKNKTLLSVATLLTSLLTSPANAISVDWKGTYRFEWTQVDRP
                     SLGTPYGMKSYGLNYLSLSPKIIATDGITIVSKFDVLANQDEAYQNAQFGSLWGQPPV
                     GSTQNRVMAQNQRSTQLLVSQLYLNLNQEYGALVLGRAPMEFGLGITHNAGNGAFDHW
                     ADTEDLVGYKFIVDNLFFMPSFGRVYDEDGAQGNTVQDMFLHLQYESKESGSLIGIMQ
                     QTRKASLGANDVPLAGSATRTGEYNVQTVSFILGREWEQFSFKIEAGFTSGNVGAQTA
                     LGEDIKNNGYGVAAELHFPRKESKMDWNLRFGLATGDDPNTANTIEGFQFDRNYDIAM
                     LMFNHRLGQQDFLGTNLIKETTTHTVANSLDDEAISNAMYVSPRLSYAWNDHLDLNNT
                     LTYAQIMTNPIAGTTGFSKDLGLELDIELVYKPTDRIQWVNQLGLLFPGAAFKNGTGA
                     GGGLENASTYGFATKAALSF"
     gene            3247..3708
                     /locus_tag="VIG33_15080"
     CDS             3247..3708
                     /locus_tag="VIG33_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272212.1"
                     /translation="MDPVSTPKKGRPQMTAIKTKTYKYGPHTFKSYFRPVGNGWEVGF
                     THQNHNYFVGNFVHRAEATKWWTMFNKEIMDFSRKFNFSKEMPFSWYCNFLSNHMYTC
                     YYAWLDKVFVKHETTFKKAYSKDFKRFQKMTKTRTRTAGADKNTGYFLKAV"
     gene            complement(3802..5502)
                     /locus_tag="VIG33_15085"
     CDS             complement(3802..5502)
                     /locus_tag="VIG33_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272213.1"
                     /translation="MNKLTDTQKKWALTACLTSVLSLNLVMNLANSSLISNYQSADFA
                     SESRYTREVAAELAAEAVKDPAKKPAPIEITKTATPKAAPKPETDSENTESAPTKKGS
                     AIRPPTPEEAANDEGSSSVEAAPEKNQAESQDPNIKWVPFVSEKGERIKVQYTQENDK
                     QTLALVPKVTQAGICYSCGKEYLLPKEFSSSASDLEAALRKAINKKAAVNNDEEKIAD
                     TSEDEDLEPQVVSRSSKINQEIGEDEEEPAPEEDKDFVALKKKCDNKEDDSRLECFAN
                     GLTKLLKDTYDNRKSKKEHRKEDVLALFKEEIQPGLISGLKDVSDLLPSRRMRTDPFA
                     DVVDTEQENRREKAQVLVENMISSISKKYNYLRQKLTAIAASVVLKSQEEAQQKLKLA
                     DQIKQTNPGQALKLQSEGYAKLRAANQSGYEIGGTLFNGLSSAQDFNYINQEQFDNLY
                     QDNYANLVNQAIQGLTTNPLTYVIPSATLSPGSRIVDGNGQEFTLTPSTSPLVQQRLG
                     RGISVVNNGLTATNLATQNAPRIVTLGGILQPGTATIQPFQATQAQTLQSLSAPRNGR
                     "
     gene            5761..6963
                     /locus_tag="VIG33_15090"
     CDS             5761..6963
                     /locus_tag="VIG33_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEY8272214.1"
                     /translation="MKLHFNRAPRPDAILLSDWNRWTWQLQKSLKSQLDFENAFALSA
                     EESQIFQKGSEVFNIRTTPYYASLAGAHPADPIRRIFMPNTLELGTPVQAMLDPLGER
                     NNNPAPRIIHRYPDRVLFLITDICSVYCRYCTRKHFTGQEQAFIKTDEYTQALNYIKT
                     HPGIREVILSGGDPLTVSDVQLDRTLYDLRQIEHVEIIRLGTRMPVVCPMRVSEDLVK
                     ILRKHKPVFLMSHFNHPREITEEAAQALELFVDHGVPVMNQMVLLNGVNNHAAIVQAL
                     SRRLLYLRVKPYYMFQCDPSRGTDHLRTSIEDSLEIQKELWGNLSGLAMPTLSLDIPG
                     GGGKTYYVPCYEVQSSPEKRSYRGWDGVDAEYVNPPQESIQKPLDTEIYLEEWRSLKS
                     AKNTCHLR"
     gene            7033..7437
                     /locus_tag="VIG33_15095"
     CDS             7033..7437
                     /locus_tag="VIG33_15095"
                     /inference="COORDINATES: protein motif:HMM:NF020360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HEY8272215.1"
                     /translation="MDIRWIQRLDNFKSAFKQLSSAVEISQQRSLTDLEKQGVIQAFE
                     FTHELAWNVIKDYFEYQGNSSINGSRDATREAFQKNLISQGDIWMEMIKSRNLTSHTY
                     NKKIAEDIVDKVIGLYAPVFKDFELKMDSLKR"
     gene            7449..7757
                     /locus_tag="VIG33_15100"
     CDS             7449..7757
                     /locus_tag="VIG33_15100"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY8272216.1"
                     /translation="MNFGLSNNVIDKINSVFKKYPSIEKVILYGSRAKGNYKPASDID
                     LTIISSTQDLSFLFRIENEIDDLLLPYKVDLSFYSLLDNENLRDHINRVGVIFYVQKP
                     "
     gene            complement(7758..8321)
                     /locus_tag="VIG33_15105"
     CDS             complement(7758..8321)
                     /locus_tag="VIG33_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272217.1"
                     /translation="MKKLSQNMLAAMIVLFFASTGLAQYGSRPSYSSSVSFGTENEII
                     TNGTFGKLLTGKSCKNCDNQTIIDISGTYLRTMNANVQAGALAEIQSISGGNSETLLT
                     LVALGVYNFDTSFKQSFFVQGGLGIYPVRKDNSLDHESKFGFLVGGGKRFPIWGNVNY
                     IPTASLVKKGDLDIGFDIEFLNFSLMF"
     gene            complement(8389..10686)
                     /locus_tag="VIG33_15110"
     CDS             complement(8389..10686)
                     /locus_tag="VIG33_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY8272218.1"
                     /translation="MKKGTHLKIMDVFAKIIQNLNPPQTEAVETLDGPILILAGAGSG
                     KTRVLTHRMANIIARGAAAPDEILCVTFTNKAAKEMEARIYKTLANIGAPIRSQLWIS
                     TFHSFCVRILRTHITLLDYKPFFGIYDSSDQLSQIKKVMKVLNINDKMYPPKNFQGRI
                     SDAKMLALTPEELRKNSRPMMDPKTLEVYSLYETEMKKANSLDFDDLLLKTYELFRMY
                     PDILEAYQDKFRYIMVDEYQDTNHIQYLIVQLLAKKHHNLCVVGDEDQSIYSWRGADI
                     SNILDFEKDFPEAKIVKLEENYRSTSNIVNAATKVIKNNLQRKDKTLFTSNAEGDLIQ
                     VREEKNEYDEARFVAKTIHSMMASGEGSYNDYAVFYRTNAQSRVLEEQMRTLGIPYRL
                     IGGVRFYERMEIKDMISYMKLSMNPSDDVAFKRVINVPTRGIGKTTVDKLEEYSIEHK
                     LSLYVAAQKATELRIFNAGTTGKIRRFLAILEELQVSATQFKLPEFYHILLDRTEYVN
                     ALKADDSPEAKARIENLDELDNAITQFAKERGEEGNLQSFLEEMALVSDVDSLNQEQN
                     SVTLMTLHVSKGLEFPFVFVVGLEENLFPSNRGVDESGNEDVEEERRLAYVGMTRARQ
                     KLWMTYTRVRRVWGQEQFNPPSRFIKEIPQDLIEFKSAIESPRFLSRYGSSNSHEEES
                     FTQSPKFRQSKDDYDSQSFPDYHSDDHGGEMSFHKGMRVRHPTFGVGSVFATEGSGEN
                     LKVSVMFANSTVKKFVVKYARLEKV"
     gene            10761..12155
                     /locus_tag="VIG33_15115"
     CDS             10761..12155
                     /locus_tag="VIG33_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272219.1"
                     /translation="MKLIVAGDLFVKLFGMNAFQSLVRVSLLVLILGCNREPETHKVS
                     GDVKTRIPFTSEGGAYSLQEVTLSGITSLYEFSGRYVNFYIYPAVADSRIYGHRPKTR
                     FLKTGDLYVPEDDLSQQVAVIYAHFQRLAALDAELGAGNLNQWPRDVGVAVRYNMGKN
                     YQTNNAFYDGSTDAMLVVPYTQNNLPIAVNGGILAHEHFHSLYFKIVEKNVFKKAMPL
                     HGEEMREEVLGAKSKGQKLFLKKPTTEDNEDFINDYHDLFSRGINEGLADFWAWIYTG
                     DPNFIQASLPSEKVSRSLELSAAEEQSFKFPNSEVWKVRVANRFNANLGDKCRFDNVA
                     YCLGTDYARTLKRFTNVVQSSRGLNSLAARKLVGASILKALPLLKEELLALKKAEYFE
                     PTKFFTLLANSVEDLTSQEKEFLETLIAKATKKEVIVSAGSLRPSAEDEAPSATITPL
                     PLVSKKDARTGDDK"
BASE COUNT         3309 a         2525 c         2845 g         3554 t
ORIGIN      
        1 gttgttgagt gatagtgctt ccgccctgaa cggcacggcc ggaggtgatg tttttgacca
       61 gagctctgaa gattcctcgg tagttgacgc ctttatgttc taaaaatcgt ggatcttcga
      121 tggccatcac tccattggag caaaaaggag gaacttttcc caaaggttgt tcttcttgca
      181 taatagggtc aattccaacg tattgggcga ctaaaggggc ttctaaggaa acttcgctga
      241 gaggctcaag aggaaaagtc ttaaaaatct gttgaattcg attttcattg ttgaatagca
      301 ggagatggat attattttgc acttggccag ggtctacgtc tttttttata aaagcgatgc
      361 aattgtcaaa attgatttct tcgggaattc gaaataaagt tttgcattca agagtactgg
      421 cttttagata atccccagga agaagacgtt gttgaaagtc tcgggagcga taattttggc
      481 cttgaagtgc tttttcggat ttatccacat cctggaggga gggtgtatag aaacgatatg
      541 gagcagcata atactctgtg ggcagaagga ttcgtttggc ttccagcttt tgatccattt
      601 cttctgaaag ggagagaaag gcgtaagaaa tgcccgctgc cagcgtgatg attgcaagcg
      661 tcacaaagat tgtaaataga aaaaaaatgt tctttaatcc tgatcgaggc atgcaccatt
      721 atgaaattat cctcccaaca aattcaagtc ttagctgaaa aaattctgaa ccattggaag
      781 aaacaaaatc ttatcgtctt caaagaagac gaaaaggttg ttcttgctcg tatggttgag
      841 ctcattaagg aagattacgc tcgcgaagcg gctctcgatc aagaggtcaa tgcgatgctg
      901 gatcaactgg agaagagcaa tcccggtgag ttccagcgtt ttaagatgtt tcccatctta
      961 aaacagaagc tcgcgaaaga aagaaaagtg attctatgat tatttcagga gatcgccaaa
     1021 atcatctagc acatatagtg acagacggtg tatggggtga cgatatcgcg gactttatgg
     1081 acgacgacca ggcgttacgg gtggcaaaac tggccattgc ggagttcgtg aaagaagacg
     1141 aggaccttga tcaaagggct cgcgataaag tcgcttcatt aaaacgcaat gtgatggaag
     1201 gcaccccgga gtgggatatt atgtataaaa agtactatca agaggagagg gttaaacgtg
     1261 gccaaaagta aaaaaaccgt agctaaaaaa tcagcaaaga agccggcaaa gaaagcatct
     1321 gcggctaaga aaacggttgt gaaaaaaact gctgctaaaa aagcagctaa aggtaaagcc
     1381 gcgcctaaga aggtcgtgaa taaaaaaacg aaaatagacc tgagccgtgt tgtgactccc
     1441 ttggatgatc gtgtgctggt tcaattagtt gagaaagaaa caatgacggc gggaggtctt
     1501 tatatccccg atacggtgtc agatgtttca gggaacctgg aaggatttgt aatttccgtg
     1561 ggacgcggcc atcgcaataa aaagggctac gttcgcccaa tggacttgaa agtcggagat
     1621 aaagttcttt tttctgaata tgcgggttct aaaattaaaa ttcaagatca agaccttctc
     1681 atcgttcgag agatagacgt tcttggtgtg attgcataaa aattatcata gggtgaataa
     1741 tgaatttaaa aaataaaaca cttctttctg tagcaactct tttgacttcg ctgctgactt
     1801 ccccagccaa cgcaatcagt gttgattgga aaggcaccta tcgctttgag tggacgcaag
     1861 tggatcgtcc ttctttgggc actccttatg gaatgaaatc ttatggccta aattatttat
     1921 ctttgagccc aaagattatc gctacagatg gtatcaccat cgtttctaag tttgatgttt
     1981 tggcgaacca agatgaagcc tatcaaaatg cgcaatttgg ttccctttgg ggtcagccac
     2041 ctgtagggtc tactcagaac cgtgttatgg cgcaaaacca gcgttccact caattgttag
     2101 tgtctcaatt atatcttaac ttgaatcaag agtacggcgc gctcgtttta ggccgagctc
     2161 ctatggagtt tggtttggga atcacccata acgctggcaa tggggctttt gatcactggg
     2221 cggatacgga agacctcgtc ggttacaaat tcatcgtaga taatttgttc ttcatgccga
     2281 gtttcggtcg agtttatgat gaggatggcg ctcaaggcaa tactgttcaa gatatgttct
     2341 tgcacttaca atatgaaagc aaagaatccg gttctttgat cggaatcatg cagcagaccc
     2401 gaaaggcaag tctgggtgcc aatgatgtgc ccctcgctgg ctcagcgacg aggactggag
     2461 agtacaatgt tcaaaccgtg agctttattt taggccgtga gtgggagcaa ttcagtttca
     2521 aaatcgaagc tggattcacc agcggaaatg tgggcgcaca aacggctctg ggtgaagata
     2581 ttaaaaataa tggttatgga gttgcggcgg aactccattt tccccgtaaa gaatcaaaga
     2641 tggactggaa tcttcgtttc ggtttggcga ctggagacga tccaaacacc gcaaatacaa
     2701 tcgaaggctt tcaatttgat cgcaactacg atatcgcgat gttgatgttc aatcatcgat
     2761 taggacaaca agattttctc ggaacaaatc ttatcaaaga aaccaccact catactgttg
     2821 cgaattccct ggatgatgag gctattagca atgcgatgta tgtttctccg cgtctttcgt
     2881 atgcttggaa cgaccatttg gatttaaata acactttaac ctacgctcaa atcatgacca
     2941 atcccattgc gggaaccaca ggttttagca aagatctcgg tttggagttg gatatagaat
     3001 tagtttacaa acccactgat agaattcaat gggtcaatca attgggtttg ttatttccag
     3061 gtgccgcatt taaaaatgga acgggtgcag gtgggggctt agaaaatgca agcacctacg
     3121 gatttgctac aaaagcagcg ctcagttttt aaaatcgcgc gcgacaagag tctagttttt
     3181 tttatgagat cgtcctggtt ttttctcata aaaaaactgt ttagggattt tcctttcagg
     3241 ttcaagatgg atcctgtttc aacacccaaa aaaggaaggc cgcaaatgac agctataaaa
     3301 acaaaaacat ataaatatgg tccacatact ttcaaatcct atttcagacc tgtaggtaat
     3361 ggttgggaag tgggtttcac tcaccaaaat cataactact tcgtaggcaa tttcgtccac
     3421 agagctgaag ccactaaatg gtggactatg ttcaataaag aaatcatgga tttctctaga
     3481 aaatttaatt tctctaaaga aatgccgttc tcatggtatt gcaatttctt atcaaatcat
     3541 atgtacactt gttactatgc ttggttggat aaagtttttg ttaaacatga gacgactttc
     3601 aaaaaagcat actctaaaga tttcaaacgt ttccaaaaaa tgacaaaaac cagaacaaga
     3661 accgctggtg cggataaaaa tactggttac tttttgaaag ctgtttagtt ttttctcgtt
     3721 caacgagagg cggcgagttc gtcatgaact cgctgaggat cactgaaaca tcactaaatt
     3781 tctctaaatt catcgacagt tctagcgtcc attgcgggga gcactcaagc tttgcaaggt
     3841 ttgcgcttga gttgcttgga atggctgaat cgtcgcggtt cctggctgca gaattccgcc
     3901 caaagtcaca attcgcgggg cgttttgtgt cgctagattc gtcgcggtca acccgttgtt
     3961 cactacagag attccccggc ccagtcgctg ttggactagc gggctggtac tcggagtgag
     4021 ggtgaattcc tggccgttcc catccacgat tcgacttcca ggggataagg tggcagatgg
     4081 aatcacatag gtcagaggat ttgtagtcag gccctgaatc gcttgattta caagatttgc
     4141 ataattatct tggtataagt tatcgaactg ctcttggttg atgtagttga agtcttgagc
     4201 acttgaaaga ccattaaaaa gagtcccgcc aatttcgtag ccgctttgat tggccgctcg
     4261 taattttgca taaccttcgc tttgcaattt caatgcttgt cctggattgg tctgtttaat
     4321 ttggtcagca agcttcagct tttgttgcgc ttcttcttgg ctcttaagca caaccgacgc
     4381 ggcaatggcg gttaatttct ggcgcaagta attatatttt ttactgatac tggaaatcat
     4441 gttctccaca agcacctggg ccttttcacg gcgattctct tgttccgtat ccacgacatc
     4501 ggcaaagggg tccgtgcgca ttcttctaga aggtaaaaga tcgctgacgt ctttaagtcc
     4561 ggaaatgagt cctggctgaa tttcttcttt gaaaagggcc aaaacatctt cttttctatg
     4621 ttcttttttg ctctttctgt tgtcgtaggt atctttaagt aactttgtaa ggccattggc
     4681 gaaacactcc aggcgcgagt catcctcttt gttatcacat ttctttttaa gagcgacaaa
     4741 atctttgtcc tcttcaggag ccggttcttc ttcgtcttct ccgatttctt ggtttatctt
     4801 agaagatcga gaaaccactt gcggctctaa atcctcgtct tccgaagtat cggcgatttt
     4861 ttcttcatca ttatttactg cggctttttt gtttatcgct tttcgaaggg cggcttctaa
     4921 atcgcttgcg ctactgctga actctttcgg caataaatat tctttcccac aactgtaaca
     4981 gattccagct tgggtcactt tagggacaag agcgagcgtt tgcttatcat tttcttgcgt
     5041 gtattgaact ttaattcttt cgcctttttc ggaaacaaac ggaacccact tgatattagg
     5101 atcttgggat tctgcttggt ttttttcagg ggctgcctct acagaggacg agccctcatc
     5161 gttggctgct tcttcagggg ttggaggtct tattgcggat ccttttttag taggtgccga
     5221 ctcggtattc tcggaatctg tctctggctt tggggctgct tttggggttg cagtcttggt
     5281 tatttctatg ggggcaggtt ttttagctgg gtctttaaca gcctctgcgg ctagctctgc
     5341 ggctacttct ctagtgtatc gggattcaga ggcaaagtct gcagattgat aatttgaaat
     5401 caaacttgag ttcgccaagt tcattacaag gttgaggcta aggactgaag tcaaacaggc
     5461 ggtcagcgcc catttttttt gagtatcagt tagtttgttc atatcatcct cctaaaatga
     5521 gcctgtcgga ctcttggttc taacactctt taaagcgaaa cccacgccaa ggggcccggc
     5581 tctctttttt tttgttaagt gtttgatttt gttagattca tttaatcttc atgaaagaaa
     5641 aataagagag agggcttgtc aaacttctgg cgtctcaccc tgagaatcag tgaaattctc
     5701 tttggtttaa aggacttagg aatgctataa ggtcccactc acgaaggacc gaatacgccc
     5761 atgaaattgc attttaatcg agctcccaga cctgatgcta tattgctttc cgattggaat
     5821 cggtggacct ggcagttgca gaaaagtctt aagtcgcaat tagactttga aaatgctttt
     5881 gccttgagtg cggaagaaag tcagattttt caaaaaggtt cggaagtttt taatattcgc
     5941 accactcctt attatgccag tctggcgggg gcgcatcctg cggatcccat tcggcgtatc
     6001 ttcatgccga atactttaga actgggcact ccagtacagg cgatgctgga tcctttagga
     6061 gagcgtaaca ataatcctgc gccgagaatc attcatcgtt acccagaccg tgtgctcttt
     6121 ttaattaccg atatttgcag tgtgtactgc cgctattgca cccggaagca tttcaccggg
     6181 caagagcaag cctttattaa aaccgatgaa tacacccagg ccctgaacta tattaaaact
     6241 catccgggaa ttcgtgaggt gattctttcc ggcggcgatc ctctgaccgt gagtgatgtg
     6301 caattggatc gcactcttta tgatctccgt cagattgaac acgtggagat cattcgtctg
     6361 gggacccgaa tgccagtggt gtgtccgatg cgggtgagtg aagatctcgt gaaaattctg
     6421 cggaaacata aaccagtttt tttaatgtcc cattttaatc atccccgcga gatcacggaa
     6481 gaggccgctc aggcattgga gctttttgtg gatcatgggg ttccagtgat gaatcagatg
     6541 gttctgctga atggcgtgaa caatcatgcg gcgatcgtac aggctttatc tcggagattg
     6601 ttatatttac gagtgaagcc ttattatatg tttcaatgtg atccttctcg cggtacggat
     6661 catctgcgca cgagtataga ggattctctg gaaattcaaa aagaattgtg gggaaatctg
     6721 tcaggcctgg cgatgccgac gctttctttg gatatccccg gaggcggagg taaaacctac
     6781 tatgtgccct gctatgaggt gcagagttcc ccggaaaaac gtagctaccg gggctgggat
     6841 ggagtggacg cggagtatgt gaatcctccg caggagtcta ttcaaaagcc tttggatact
     6901 gaaatctatt tggaagagtg gcgctctttg aagagcgcca agaatacttg tcatctgcgc
     6961 taactttagc tctttaaagt tgatcaattt acaattacaa taggatcaat aggttttctc
     7021 ggaggagatt ttttggatat tcgttggatt caaagattag acaactttaa atcggctttc
     7081 aagcaactgt cttctgccgt tgaaatttca caacaacgaa gtttgactga tttagagaaa
     7141 caaggcgtca ttcaggcttt tgagtttaca catgaactag cctggaacgt gatcaaagac
     7201 tactttgaat atcaagggaa cagttcaatc aacggctcaa gagacgcgac tcgagaggct
     7261 tttcaaaaaa atctgatatc ccagggggat atttggatgg aaatgattaa aagccgcaat
     7321 ttgacctcgc atacatacaa taaaaaaatt gctgaagata ttgtcgataa agtaattgga
     7381 ctctacgcac ccgtgtttaa agatttcgaa ttgaaaatgg attcattgaa gcgatgactc
     7441 aagagaagat gaactttgga ctttcaaata acgtaattga taaaataaac tcggtcttca
     7501 aaaaatatcc gtccattgaa aaggtgattc tctacggctc gcgcgctaaa ggtaattaca
     7561 aaccggcctc agatatagac ttaactataa tctcttcaac tcaagactta tcttttttat
     7621 ttcgaataga aaacgaaatc gatgatttgt tattgcctta taaagtggat ctttcttttt
     7681 attctttatt ggataatgaa aatctacgtg atcatatcaa tcgagttggc gttatttttt
     7741 atgttcaaaa gccttaatta gaacatcaaa gagaagttca agaattcgat atcaaaaccg
     7801 atgtccaaat cgcctttttt aaccaatgaa gctgttggaa tgtagttcac attgccccag
     7861 atcgggaagc gcttgccgcc acccactaag aaaccaaatt tgctttcatg atcgagacta
     7921 ttgtcttttc gaaccggata aatccccaaa ccaccttgaa caaagaaaga ctgcttgaag
     7981 ctcgtatcaa aattgtaaac tcccaacgcc accaaggtca gaagggtttc gctgttacct
     8041 ccgctaatag attggatttc ggcaagagca ccggcttgaa cattggcatt catggttctg
     8101 aggtaagtcc cagagatatc aatgatcgtt tggttgtcgc agtttttaca ggatttcccc
     8161 gtgagaagtt tgccgaaggt gccattagtg atgatctcgt tttcggtccc aaagctcact
     8221 gaagaactat acgatggacg actcccatat tgggctaagc cagtagaagc aaagaaaagg
     8281 acgatcatag cggcaagcat attttgactg agttttttca agctggactc cttagattgt
     8341 tatctattta taataaaaac tttgtcacag ccgtacttac agtcaaggct aaactttttc
     8401 caggcgcgcg tatttaacca caaatttttt gacagtactg ttggcaaaca tcacgctgac
     8461 cttgagattt tcaccagagc cttcagttgc aaacacggag cccactccaa aagtgggatg
     8521 acggactcgc atccccttat gaaaggacat ttcgccgccg tgatcgtcgc tatggtaatc
     8581 cggaaagctt tgggaatcat aatcatcctt gctttgacgg aatttaggtg actgcgtaaa
     8641 gctttcttct tcgtgactgt tcgatgagcc ataacgggac aaaaaacgag gactttcaat
     8701 ggcggattta aattcgatta aatcttgagg gatttctttg ataaaccgtg acggtggatt
     8761 gaactgctct tgcccccaga cccgacgaac ccgggtgtac gtcatccata gtttttgtcg
     8821 tgcccgagtc atgccgacgt aagctaggcg tcgctcttct tccacgtctt cattgcccga
     8881 ctcatccacg ccgcgattgc ttgggaagag attttcttca agtcctacga caaagacgaa
     8941 ggggaactca aggcctttgg aaacgtggag ggtcatcaaa gtcacagagt tttgttcttg
     9001 attgagggaa tctacatcag agaccaaagc catctcttca aggaaggact gcaaattgcc
     9061 ttcttctcca cgctctttgg cgaattgagt aatggcattg tccaattcgt ccaagttttc
     9121 gatgcgggct ttggcttccg gagaatcatc ggctttcaac gcgtttacat actccgtgcg
     9181 atctaaaagg atgtgataaa attctggcaa cttaaactga gtggcggaga cttgcagttc
     9241 ctccaaaatc gcaaggaacc tgcggatttt ccccgtggtg ccggcattga atattctcag
     9301 ctccgtggcc ttttgagcgg cgacatacag actgagcttg tgttcaatcg agtattcctc
     9361 aagcttgtcc acagtggttt tgccaatacc gcgagttgga acattgataa cccgtttgaa
     9421 ggccacgtcg tctgaagggt tcatggaaag tttcatgtag gaaatcatgt ctttgatttc
     9481 catgcgttca tagaagcgca cgcctccgat aagacggtaa ggaatgccca gggtacgcat
     9541 ctgctcttca agcacccgag actgagcatt ggtgcgatag aaaaccgcgt agtcgttgta
     9601 agagccttca ccggaagcca tcatggagtg gatggttttg gcgacgaagc gagcttcatc
     9661 gtactcattc ttctcttcgc ggacttggat caaatcccct tcggcattgg aagtgaagag
     9721 cgtcttgtcc ttcctctgca gattgttctt aatgacttta gtcgcggcgt tcacaatatt
     9781 tgaggtggaa cgatagttct cttccagctt aacgatcttg gcttcaggaa agtccttttc
     9841 aaaatccagg atattggaaa tatccgctcc acgccaactg taaatcgact gatcctcgtc
     9901 gccgacgaca caaaggttgt gatgcttttt agccagcagt tgcacgatga gatactggat
     9961 atgattggta tcttgatact catcgaccat gatataacgg aatttatctt ggtaggcttc
    10021 caagatgtca ggatacatac ggaaaagctc ataagttttc agaagcagat catcgaagtc
    10081 gagactgttg gctttcttca tctctgtttc atagagtgaa taaacttcga gagttttagg
    10141 atccatcatg gggcggctgt ttttgcgcaa ttcttcggga gtcagcgcca gcattttggc
    10201 gtcgctaatg cggccttgaa aatttttagg aggatacatc ttgtcattga tgtttagcac
    10261 cttcatgact tttttgattt ggctcaactg atcagaggag tcatagatcc caaaaaatgg
    10321 cttataatcc aaaagagtga tatgggttct gagaatacgg acgcaaaaac tatggaaagt
    10381 gctaatccac agctggcttc gaataggggc tccgatattc gcaagggttt tataaatccg
    10441 cgcttccatt tctttggcag ctttattggt gaaggtgacg cagagaattt catcgggagc
    10501 cgccgcccca cgggcaatga tattggccat tctgtgagtc aaaacccggg tttttcctga
    10561 gccagcaccg gctaaaatta atatagggcc gtctaaagtt tctacggctt cagtttgggg
    10621 aggattcaag ttttgtataa tttttgcaaa tacatccata attttgaggt gcgtgccctt
    10681 tttcatgtat aaataatgca gctaaataac gcagccagac aagtttggtt tgcgatattt
    10741 ttttgtcatg gaaactgttc ttgaaactta tagtcgccgg agatcttttt gtgaagcttt
    10801 ttggaatgaa tgcctttcaa tctcttgtcc gagtctcact cttagtgctt attttaggat
    10861 gcaatcggga gccggagact cacaaggttt ctggagatgt caaaactcgc attcctttta
    10921 cctctgaggg cggtgcttat agtttgcaag aggtcacttt atcgggaatc acttcgcttt
    10981 acgagttctc tggccgctat gtgaactttt atatttaccc cgcagtcgca gattcaagaa
    11041 tttatggaca ccgaccaaag acacgatttc taaaaaccgg cgatctttat gtccctgaag
    11101 atgatttgtc ccaacaagtc gctgtgatct atgcgcattt ccagagattg gcggccttgg
    11161 atgcggagct gggggcaggg aatctgaatc aatggcctcg ggatgtcggc gtggctgttc
    11221 gttataatat ggggaagaat tatcaaacaa ataatgcctt ctatgacggc tccaccgacg
    11281 ctatgttagt agtcccctat actcagaata atttgccaat tgcggtgaac ggaggaattt
    11341 tagcccacga gcattttcat tctttgtatt tcaaaatagt cgagaagaat gtttttaaga
    11401 aggcaatgcc tttgcacggg gaagagatga gagaggaagt tttaggggcg aagagcaagg
    11461 gacagaaact ctttctcaaa aaaccgacga cagaagacaa tgaagatttt attaacgact
    11521 accatgatct tttctcccga gggatcaatg aaggtctcgc tgatttttgg gcgtggatct
    11581 atacggggga tcctaatttt atacaggctt ctttgccctc agagaaagtc agtcgcagcc
    11641 tcgagctgag cgcggccgaa gaacaatctt ttaaattccc taacagcgaa gtttggaaag
    11701 tacgagtggc caatcgcttt aatgcgaatc tcggagataa gtgccgcttt gataacgttg
    11761 cttattgttt gggtacggac tatgcgcgaa ctttgaaacg ttttacgaat gtggttcaat
    11821 cgtcccgagg gttgaattct ctagcggcac gcaagttggt cggcgcctct atacttaagg
    11881 cgttgccatt actaaaggaa gagctcttag ccttaaaaaa ggctgagtac tttgaaccca
    11941 cgaagttttt tactctctta gcgaactcag tcgaagacct gacatcccaa gaaaaagaat
    12001 ttcttgaaac actcatagcc aaggcaacaa aaaaagaggt catagtcagc gccgggtctc
    12061 tgcgtccttc cgctgaagac gaagcgcctt cggcgacgat cactcctttg cctttggtct
    12121 ctaaaaagga tgctagaaca ggagatgata agtgaagtct ttttctgtca tgtggggtgt
    12181 atttttctgt agctttacag ctttcgccgg gccatcgaaa tggcacgcag tgc
//
LOCUS       DATBTE010000394         4211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2378493, whole genome shotgun sequence.
ACCESSION   DATBTE010000394 DATBTE010000000
VERSION     DATBTE010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2378493"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VIG33_15120"
     CDS             <1..304
                     /locus_tag="VIG33_15120"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY8272220.1"
                     /translation="QVVRLFIEKCPEQLATIREAIEQQDGELLRQTAHSIKGAIGNFT
                     DRAAFEAARHLEQLGRDSDFSLALDGLFRLEQELQELKSALNALVCYFPEQTSSES"
     gene            713..2740
                     /locus_tag="VIG33_15125"
     CDS             713..2740
                     /locus_tag="VIG33_15125"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEY8272221.1"
                     /translation="MSAPKILLVDDETNALNALQRLLSGENFEITATDSPFQALHLAS
                     KEEFTIILSDYRMPHMEGTMLLQKVKEISPASMRILLTGHADMETAIAAINEGEVDRF
                     LLKPWDDTEMIRILKLSVEHFEVIKDQKTVYGQNSDLKKKNQSLVEKNIEQAFQNKRL
                     CHALDQSYIISINALAKSIKLYESHLWHHSLAVKMICQKIAKLLGLPPQKSTQLAVAA
                     ILHDIGKIGLPHTVLLKPEKMLSPSEKELYQRHPLNGYDIVSQIPYFQEAAVFIRHHH
                     ECFDGTGYPEGLKGTDIPAGARIIAVANTYAKIKKGHPFSLDASYQEAMTFLEGQGGT
                     RFDPDAISLLKECIAVSTGQKRNPARQETIDYWQHVVVSEPEKVAMLAAISESADGTP
                     DLSNMKLHSLDDREQLLINQPDAVFSDLKPLDIKPYLDSADSTFIWEKDARSSKFLTP
                     SDNPIGKVRTESLTSTDELIDDWSLPEILDWQVESSLMTEVPEYPSETRLEVISKFPE
                     SEVSGYEPEQMISPDHSQAAKKVLDAIMELPASEVSCIEPEPLITSMQSKKNTLGVPE
                     TEVKSSGQSVLNGQNPANSLTDFHLHKARNQPGYRDAYRRAKQKYKEVVIPAMYLRVG
                     MVLSRDIMTYQGVKLLQKGTTIGSDQLDGIKSYLKRNNLQELEIYILSYAG"
     gene            complement(3100..>4211)
                     /locus_tag="VIG33_15130"
     CDS             complement(3100..>4211)
                     /locus_tag="VIG33_15130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEY8272222.1"
                     /translation="LFTQYCRFEAVQPVREEELPREEWTELPKEQQAEEHPLVILNVT
                     SPEKFPTSIKSYLKHNHVQSAFAIEIKYRRVVFGRLVLHQCSYPRIWTGQEISFLEIL
                     ATHIGAALYQVKLYQQEKQAKQEAEEANRQKSKVLSFVSHDFKNPLASMKRFIDILEN
                     DKSDLLSEKHRELIEYIAEGVYQLRNMVMDILDKARLDEGRITPITEWIEVRSFIDEL
                     KPMFSSMASQRNIKVSLECQPELTALKADPTHLRQILINLVSNAVKYNRMDGEAFLRL
                     YKSEDGQSAVIEVQDTGLGISEEKIPQLFTEYFRGDLSQSNLVEGTGLGLAFIKKLIE
                     LHGGSIVVESEVGVGSTFKVMLPFMAKNHDRDPQN"
BASE COUNT         1186 a          962 c          982 g         1081 t
ORIGIN      
        1 tcaagtggtc agattgttca ttgaaaaatg ccctgaacag ctggcgacaa tacgagaagc
       61 tattgagcag caagatggcg aactgttgag acaaacggct cattcaatca agggagcgat
      121 cggcaatttc acggataggg ctgcctttga agcggcccgg cacctggaac agttgggtcg
      181 tgacagtgat ttttctctgg cgttagatgg acttttccga ttggaacaag aacttcagga
      241 actcaaaagt gcattgaatg ccttggtctg ttatttccca gaacagacca gttcagaaag
      301 ctgaccgtcc aaaccgaaag agatccaagc agttacaaac aatactggta tctcggttta
      361 gcctggctgg aactcaaaaa acatatcgga taagctctgg tttcacaatc ctccatgttt
      421 tagcaggttt tccagagggg gggtctattg cgcagtaggg tttaatacca aagagctatg
      481 tttcaccggc taaaactctg ggtattaatt aattaatcaa gcgctaaata taatattggt
      541 cgctgtcgcc agaatgatag tgaagatcat gttgtcatca taaataaggc aagctgtgat
      601 tatgcctgac aagtggcaag tatcaggcag gatacgggca taaaacaaag taatccgatg
      661 cctgatgcta aaaactgatg atgtttagac acatttaaag gagacggtca ccatgtcggc
      721 ccctaaaatt cttctcgtgg atgacgagac aaacgccctg aatgctttgc agcgcctcct
      781 aagcggtgag aattttgaga ttactgctac ggattctccc ttccaagcct tgcatctcgc
      841 ctcaaaagaa gagtttacca tcattctgtc tgattataga atgcctcata tggaaggaac
      901 catgctgctg cagaaggtta aggagatttc cccggcttca atgagaatac tgttgaccgg
      961 ccatgcggat atggagaccg ctatcgccgc tattaatgaa ggggaagtcg atcggttcct
     1021 gctaaaaccg tgggatgaca cggaaatgat cagaatttta aagctttccg tggagcattt
     1081 tgaagtcatc aaagatcaaa aaacagttta cggccagaat agtgatctta aaaaaaagaa
     1141 tcagtctctg gtcgagaaga atatagagca ggctttccaa aacaagcgtt tatgtcacgc
     1201 actggatcag agctatatca ttagtattaa tgctttggcc aaatcgatca aactttatga
     1261 atcccactta tggcatcact cccttgcggt taaaatgatc tgccaaaaaa tcgcaaaatt
     1321 actcggccta ccgccacaaa aatcaaccca gctggcggtt gcagccattt tgcatgatat
     1381 cgggaaaatc ggactgcccc ataccgtcct actgaaaccg gaaaaaatgt tgtctccatc
     1441 tgaaaaagaa ttgtatcaaa gacaccccct gaatggatac gacatcgtta gccaaatccc
     1501 ctactttcaa gaagcagcag tgtttatccg gcatcatcac gaatgctttg atggtaccgg
     1561 atatccggaa ggcttgaagg ggacggatat tcccgccgga gcgcgcatta tcgcagtcgc
     1621 taatacctat gccaaaataa agaaagggca tccattctcc cttgacgcct cctatcagga
     1681 agcgatgact ttcctggagg gtcaaggcgg tacacgattt gatcccgatg ccatcagcct
     1741 cctgaaggaa tgtattgctg tctcgacggg acagaagagg aatccggcaa gacaggaaac
     1801 aatcgactat tggcaacacg tcgtggtgtc ggagcctgaa aaggtggcaa tgctggctgc
     1861 gatttccgag tcggcagatg ggacgcccga cctgtcgaac atgaagttgc attccctgga
     1921 tgatcgtgag cagttgctaa ttaatcaacc agatgccgtc ttttccgact tgaagccatt
     1981 ggatattaag ccctacttgg attcggcaga cagtactttt atttgggaaa aggatgctcg
     2041 ctcttccaaa ttcctgaccc catccgacaa ccctatagga aaagtgcgga cggagagtct
     2101 gacgagtacg gatgagctca ttgacgactg gagtttaccg gaaatattgg attggcaggt
     2161 tgagtcgtct ttaatgaccg aggtaccgga atatccatcc gagacaagat tagaagtcat
     2221 ctccaagttt cctgaaagcg aagtctcagg ctatgaacca gaacaaatga tatcgccgga
     2281 tcactcccaa gcagcaaaaa aagtattaga cgccatcatg gagctgccag caagcgaagt
     2341 gtcttgcatc gaaccagagc cactcataac gagtatgcaa tccaagaaga atacccttgg
     2401 tgttccggaa acggaagtta aaagctctgg gcaaagcgta ttgaatggcc aaaatccggc
     2461 aaacagtctt actgacttcc atttgcacaa agctaggaac cagccggggt atagggacgc
     2521 ctaccggaga gcaaaacaaa aatacaagga ggtggtcatt cccgccatgt atttacgcgt
     2581 cggaatggtc ctatctaggg atataatgac ctatcaggga gtcaaacttc ttcaaaaggg
     2641 cacgaccatt ggttctgatc aattagacgg gattaaaagt tacctgaagc gaaataatct
     2701 gcaagagctg gaaatttata ttctaagcta tgcagggtag attgaatgga gggtggtgct
     2761 ttcttccgag tgaactgttc tggacccagg aatgaagacc attcaaaagt tgagtctgtc
     2821 ctgttgacac tctttggtgc cgtcctcagt gtcttccagc acttgaaacg caaaaaatct
     2881 catcatactg aggatagtac cgtttcagcg ccaaaaaggg accaccctag aaccctcaag
     2941 gtgcctatct ggtaccgttt acggcagagc cctttataag aagatcggtg caggttaatt
     3001 ttccaccctc ttgatgagtc tatatttata ctatgggtgc ttttgagaag caagtggaat
     3061 cgattcaaaa caaaagaccg ctcatttcga tgaggtcttt taattctgtg ggtctcgatc
     3121 atgattctta gccatgaatg gtagcatgac tttgaatgtt gagcctactc caacctccga
     3181 ttccacaacg atactacctc cgtgaagctc aatcagcttc ttaatgaagg cgagacccag
     3241 cccggttccc tctaccagat tcgattggga tagatcccct ctgaagtact cggtaaagag
     3301 ttgaggtatt ttctcttccg atatcccaag gccggtatcc tgaacctcga ttacggcgga
     3361 ctgcccatct tcggatttat agagtcttaa gaaggcttcg ccgtccatcc ggttgtactt
     3421 tacggcatta ctgacaagat tgatcaggat ctggcgcaaa tgagtcggat ccgctttgag
     3481 ggcagtcaac tccggttggc attcaaggct aaccttgatg ttccgctggg aggccatgga
     3541 actgaacatc ggttttagtt catcaataaa cgacctcacc tcaatccatt ccgtgatggg
     3601 ggttatccgc ccttcatcca ggcgggcttt atccaggata tccatcacca tgttgcgtag
     3661 ctggtacacg ccctcggcaa tatattcaat gagttcccga tgtttttcag acaggagatc
     3721 gcttttatca ttttccagga tatcaataaa ccgcttcatg ctggctaatg ggttcttgaa
     3781 atcatgagaa acaaaagaaa gtacttttga tttttgccgg ttggcttctt cagcttcctg
     3841 cttggcttgt ttctcctgtt gatacaattt tacctgatat agagccgccc cgatgtgtgt
     3901 ggccaaaatc tccaggaaac tgatctcttg tcctgtccag atgcggggat aactacactg
     3961 atgaaggacc agccgtccga atacaacacg ccgatacttg atttcaatgg caaaagcaga
     4021 ctgtacatga ttatgcttta gataggactt gatgcttgtc gggaattttt ccggcgatgt
     4081 aacgttgaga atcaccagag gatgctcttc tgcttgttgc tctttcggca attccgtcca
     4141 ttcctccctg ggaagctctt cttccctgac aggctggaca gcttcaaaac ggcaatattg
     4201 agtaaaaagc c
//
LOCUS       DATBTE010000395         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_738384, whole genome shotgun sequence.
ACCESSION   DATBTE010000395 DATBTE010000000
VERSION     DATBTE010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738384"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VIG33_15135"
     CDS             complement(<1..509)
                     /locus_tag="VIG33_15135"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY8272223.1"
                     /translation="MTEVKSILKPMRLRNTEVRVPIIQGGMGIGLSSYTLAGAVAREG
                     GLGVLSSAGLDRILAQRHGKKFKAREAAAQDVRDAKIIGKGGAIGMNIMVAVVNQYED
                     SVLGSMDGGVDAIISGAGLPMALPEIALRHERNNDVALIPIVSSGRAMEIIFKRWKRT
                     GRMPDAVVV"
     gene            737..>1559
                     /locus_tag="VIG33_15140"
     CDS             737..>1559
                     /locus_tag="VIG33_15140"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEY8272224.1"
                     /translation="MRSQWKRLLWAATLSGICLMATFTWYHFTKSKSHTNDNEKPLAY
                     VGKINDEIQRRPATRLLWQEVGTGDPLFNGEAIRTSEKGEVRIQFADSSRYLDLEPES
                     LIVIQQNKGEIALDLMEGSLFVAAAKDEAEAEGTALVLNSASGKVDLSKASVNLSKSG
                     TNQMDIQVLGGSASIKDKSGKDKVLSSGNIGGPNVNNSSQFNKADLQILSPIPGKVAY
                     IDADDKENLTFKWKGFPAGLKVSLFTGPTRKDMKELTTATAPEENSFKTALPLGKP"
BASE COUNT          457 a          396 c          307 g          399 t
ORIGIN      
        1 tccacgacaa ccgcgtctgg catacgacct gtgcgcttcc aacgcttgaa aataatttcc
       61 atggcacgac ctgaagacac aatagggatc agcgccacgt cattatttcg ttcatgacgg
      121 agagcaattt ccggaagggc cattggcaac cctgctccac tgatgatggc atcaacgcct
      181 ccatccatag aacccaaaac ggagtcttcg tattgattga ccactgccac catgatgttc
      241 ataccaatag cgccgccttt gccaataatt ttagcgtcac gcacatcttg agcggcggct
      301 tcgcgtgctt taaatttctt accatgacgc tgagccagaa tgcgatctag accagcacta
      361 gacaaaacac cgagtcctcc ctcacgcgcc acagccccgg ctaaagtata gctagataaa
      421 ccgataccca ttccgccctg aataatagga actcgaactt cggtatttct tagcctcatc
      481 ggcttcaata tcgacttcac ttcagtcatc ggccacccct ttacttaaaa aatgccagca
      541 cctacgctta aacaatcgaa aagaacaata acttaaataa tatgatttag tatagataaa
      601 atccctctca tatcatatct gctttggctg actttgatcc ctaaaatcga aactttcctt
      661 tttctccaca gcccttagtc taagtaccct aaactattag gctatttaag ccgaaacagc
      721 caagaggagg tctcacatga gatcgcaatg gaaacgttta ttatgggctg ctacgctatc
      781 tggcatttgc ttgatggcaa catttacttg gtatcatttt accaaaagca aatcccacac
      841 aaatgacaac gaaaaacccc ttgcctatgt gggtaaaatc aatgacgaga ttcaacgtcg
      901 tcccgcgaca cgcttactgt ggcaagaagt gggtaccggg gatcctttgt ttaatggaga
      961 agctattcgt acttccgaaa aaggggaagt gcgcattcaa tttgctgatt ccagtcgcta
     1021 tttagatttg gaacctgaat cattaatcgt gatccaacaa aacaagggcg aaattgcctt
     1081 ggacttgatg gagggtagtt tgttcgtggc tgcggctaaa gacgaagccg aagctgaagg
     1141 aacggctctg gtccttaact ctgcctctgg aaaagttgac ttgagtaaag cctccgtaaa
     1201 tctttctaag tctggcacca atcaaatgga tatccaagtc ttaggtggct ccgcctcaat
     1261 caaagacaaa agtgggaaag ataaagttct ttcttccggt aatatcggag gacccaatgt
     1321 caataactcc tctcaattta ataaagcaga cctacaaatt ctttctccga tcccaggcaa
     1381 agtcgcttac attgatgccg acgataaaga aaacctcact tttaaatgga aaggttttcc
     1441 cgccggactc aaggtctcgc tttttaccgg ccctactcgc aaagatatga aggaattaac
     1501 gacagccact gcgcctgagg aaaactcttt taaaacggct cttcctttag gaaaaccct
//
LOCUS       DATBTE010000396         4253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2240844, whole genome shotgun sequence.
ACCESSION   DATBTE010000396 DATBTE010000000
VERSION     DATBTE010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2240844"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(393..1199)
                     /locus_tag="VIG33_15145"
     CDS             complement(393..1199)
                     /locus_tag="VIG33_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEY8272225.1"
                     /translation="MTAPNPSANPFNQQQAIPGVKHIIAVSSGKGGVGKSTIAVNLAA
                     SLGQKNKVGLLDADIYGPSIPRMLGSLNQKPSISADQRLEPIVRYGIKLMSIGFLIEE
                     SSAVVWRGPMLFKAMDQFLRDVNWGELDYLIVDLPPGTGDVQLSLAQKVPVAGAVMVC
                     TPQNVALTDVKRGVDMFQRVGIPMLGLIENMSYMINPVNGEKIQMFPKGELESFMASK
                     SIPKLGEIPFNPSIGLSCEAGIPIIESNKSGAEAQEFFKVADRIREILPV"
     gene            1250..1681
                     /locus_tag="VIG33_15150"
     CDS             1250..1681
                     /locus_tag="VIG33_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272226.1"
                     /translation="MNSQQFLLLNIVILLFFIIWFLAGRRGAAKKTTQLQMKRGNGSQ
                     GGVLQQTTGVQSRYSHPRFQKYLEQNAALENAPADSVGKDLNILFNYNGHTWDAYEVL
                     GLPAGASLVDVTRAYQQMLKSASLESQEFLETAFKTILAKK"
     gene            1785..2588
                     /locus_tag="VIG33_15155"
     CDS             1785..2588
                     /locus_tag="VIG33_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272227.1"
                     /translation="MKRILSLFLSLSFSANLSWAQEKKEAVKEPGKKETVVIPSRESK
                     DEDILKGLDYPELQVVPRASDRLLMETQNEREYGWVAFWPYQVSSIATLLAGNRLRGN
                     YKIDMSDQEKKDNDTLALSAMAVGIGGLGLSVFLIKSDLYGDSFRRIKATKVTDKRSE
                     LLRERLAEEALEKPAHLINMLTTVSVTLNVLANVAVTSQGNNDVRGYAVIGLATAFLP
                     WMFSNRYVDNWNKQLEYKRKIYTPFIGFDWQYKPKAKEFAPHLSANWSF"
     gene            2588..3457
                     /locus_tag="VIG33_15160"
     CDS             2588..3457
                     /locus_tag="VIG33_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272228.1"
                     /translation="MKGLVALGVCVLFMPSAQAETVLKSPQERENTYLPLPPKPCESF
                     KEFQRAYESLVSDNDLALGEEKAARLALQVSQGCNGSAQRFSKAYEMMKKTGVALTKA
                     VEVGLDFSGLDDEHLKNFQEVFSKMYLENSFDFDFATAYRVSFQLSRDYEGKAQDVRE
                     DFTKLVKFCIQSDQAALPLRQCAELALKIVEHTPKFPGGLYSSFEKFYTFLRTDKRMG
                     FSVLDSLNLVPRVFAYGPKAPENFKEGIEYALSSKKITIDHPNALNLALRLAKQSLPT
                     PTKEEINEAPTKK"
     gene            3435..>4253
                     /locus_tag="VIG33_15165"
     CDS             3435..>4253
                     /locus_tag="VIG33_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272229.1"
                     /translation="MKLLRRSSFFCTFIGLFLIGHVGFAEELAAKESEKVPVELEWEK
                     VLEAKLYELEFQNFNGKTLNVFKSPNHIFKFRFKVGKYKVRSRVADARKVYGDWSVLT
                     EFTVQPKPVHVTEKSINTKGVIDPKTLTTEVAIQWGEAAGALHFRVKVFNANSEVVRD
                     EIVNSHSYKTQLGAGEYTTILISIGADGIESDPVPLPGKVVIETVQLAKPVIIFDEVV
                     DAKNPKVKIKRLPLSKGIPTLRWKEASLVIDTVGTLEYRYFFGEEWIPVDAFSSK"
BASE COUNT         1168 a          897 c         1034 g         1154 t
ORIGIN      
        1 aaaagaagga tctaaatcct tagagtataa ccgctaggaa cctcctattg attagatttc
       61 ctgagttaaa ctattcctcg aaattttaaa ctttttttgc agtatctccg tctcatcagt
      121 gaaccgtgac cgcctcatca cccgcatcgt cacttgggtg acttatcatt gtgatcgcgt
      181 ttgcgactct tcatatgacc cctcccgttt tgctggccct cagagctgaa gttaaccggg
      241 ccttcacgct tacaaaacta ggatacctga aacgaggcct tattttcaac aaagtaaatc
      301 acaattctca aatgaattcg tgtacttacg tttcattatg agaatttcag atactctctt
      361 tgaacttcca agcggaacgc ggaaggagtg ttttaaactg gcaagatttc tcgaattcga
      421 tcagctactt tgaaaaattc ctgggcttca gcgcctgatt tattgctctc aataatcgga
      481 attccggctt cgcaagagag acctattgaa ggattgaaag gaatttcacc aagcttaggg
      541 atcgacttag aggccataaa agactcgagt tcgcccttag gaaacatctg gattttttct
      601 ccattaacgg ggttgatcat gtaagacatg ttctcaatga gtcctaacat gggaatgcct
      661 acgcgctgga acatatcaac gcctcgttta acatccgtga gggccacatt ttgcggggtg
      721 cagaccatga cggctcctgc cacgggaact ttttgcgcca gggatagttg gacatcgccg
      781 gtgcctggag gaaggtcgac aataaggtaa tccaattcac cccagtttac atcgcgaaga
      841 aattgatcca tggccttaaa gagcatgggg ccacgccaaa ccactgcgga actttcctct
      901 atcaaaaagc cgatactcat caatttaatt ccatagcgga cgatgggctc gagtctttga
      961 tctgcagaga tgctcggttt ttgattcagg cttcccaaca tccgaggtat acttggtcca
     1021 taaatatcag cgtccagtaa gcccacttta tttttttggc cgagagatgc ggctagatta
     1081 acggcaatcg tgctttttcc gacaccacct ttacctgaac taacggcaat aatgtgtttc
     1141 actccgggaa tagcctgttg ttgattaaaa gggttggctg acgggttggg ggcagtcata
     1201 agaactccta cttgataatt ttgcgctctc caccaataat gaaaattaag tgaatagcca
     1261 acagttttta cttcttaata tcgttatttt gcttttcttc atcatctggt ttcttgccgg
     1321 gcggcggggg gcagcgaaaa agaccactca gctgcagatg aagcgaggga atggctcaca
     1381 aggcggagtt ctacaacaga caacgggtgt tcaaagtcgc tatagtcatc cacgttttca
     1441 aaaatacctt gagcagaatg cagccttgga aaatgcgcct gctgacagcg tcggcaaaga
     1501 tctcaatata ttgtttaatt ataatggcca tacctgggat gcctatgagg tcctggggct
     1561 tcccgcagga gccagccttg tggacgtcac acgggcttat caacagatgt taaagagtgc
     1621 ttcattagag tctcaggagt ttttagagac tgccttcaag accattctcg ccaaaaaata
     1681 aaaggctcta ctttaggtgt agtcttaggg cggtccagta cactccagag ccattttcgt
     1741 ttattctaaa tcccataaaa caccgactat atatagggaa gaacatgaaa agaatattgt
     1801 ctttattttt atccttgtct ttctccgcca atctttcgtg ggctcaagaa aagaaagaag
     1861 cagtgaaaga gccaggcaaa aaagaaacag tcgtgattcc ttcacgggaa tccaaagatg
     1921 aggatatttt aaaaggcctc gactatccgg agttgcaagt ggtccctcgg gcgagtgatc
     1981 ggttgttgat ggaaacgcaa aatgagcggg aatacggatg ggtggcgttc tggccctatc
     2041 aagtttcctc aattgcgact ttgctggcgg ggaaccgact ccggggaaat tacaaaatag
     2101 acatgagtga tcaggaaaaa aaggacaatg acacgttggc tttgtcggca atggcagtgg
     2161 gcattggtgg cttgggtctt tccgttttct tgattaagag tgatctttat ggcgactctt
     2221 ttcgtcgcat taaggcgaca aaggtcactg ataagcgcag tgaattgttg cgtgaacgtc
     2281 tggcggaaga ggctttggaa aagccagcac atttaataaa tatgctgacc acggtttcgg
     2341 tgactttgaa tgtcctggcc aacgtggcgg tgaccagtca aggcaacaac gatgtgcgag
     2401 gctacgcagt gattggtctg gcgacagctt ttctgccgtg gatgttcagc aatcgctatg
     2461 ttgataattg gaataaacaa ttggaataca aacgtaaaat ctatactccg ttcattggtt
     2521 ttgattggca atataagcct aaagccaaag agttcgcacc gcatctgagt gcgaattgga
     2581 gtttttaatg aaaggcttag tcgcccttgg tgtttgtgtt ttgtttatgc cctcggcgca
     2641 ggctgaaacg gtgctgaagt ctcctcagga acgcgaaaat acctatttgc cgcttccacc
     2701 aaaaccgtgc gaaagtttta aggaattcca aagagcttac gagtccctgg tcagcgacaa
     2761 tgacttggca ttgggcgaag aaaaagcggc gcgtttggct ttgcaagtta gccaaggttg
     2821 taatgggtcc gcacaaaggt ttagcaaagc ttacgagatg atgaaaaaaa ccggagtggc
     2881 tctgaccaaa gcggtggaag tcggactgga tttttctggg ctggatgatg aacaccttaa
     2941 gaatttccaa gaagtgtttt cgaaaatgta tctggagaac tcctttgatt ttgattttgc
     3001 aaccgcctat cgagtgagct tccaactttc tcgggactac gaaggcaaag ctcaggatgt
     3061 gcgcgaagac tttaccaagc tggtgaaatt ctgtattcag agtgatcaag ccgcattgcc
     3121 cttaaggcaa tgcgcggagt tggccttaaa gatcgtcgag catactccga agttcccagg
     3181 gggattgtac agctcgtttg aaaagtttta tacatttttg cgcacggata aacgcatggg
     3241 tttttccgtg ctggactcct tgaatttggt accacgggtt tttgcttatg gacccaaggc
     3301 gccggaaaat tttaaagaag gcatagagta cgccctcagc tctaaaaaga tcaccattga
     3361 tcatcccaat gcgctgaatt tggcgttgcg tttggcgaag cagtctttgc caacgccgac
     3421 caaagaggaa atcaatgaag ctcctacgaa gaagtagttt tttttgtacg ttcattggtc
     3481 tgtttcttat aggccatgtg ggcttcgcgg aagagttggc ggcaaaggag tcagaaaaag
     3541 ttcccgtaga gcttgaatgg gaaaaagttc tggaggcgaa actctatgaa ctggagtttc
     3601 aaaatttcaa cggcaaaacc ttgaatgttt tcaaatcccc caatcacatt tttaaattca
     3661 gatttaaggt cggaaaatac aaagtccgca gtcgcgtagc ggatgctcgt aaagtttacg
     3721 gtgattggtc agtattaacg gaatttacgg tacaacccaa gccggttcac gtgactgaaa
     3781 aaagcatcaa cacgaaaggt gttattgacc ctaagacatt aaccacagaa gtggcgatcc
     3841 agtggggcga agctgcgggg gctcttcatt ttcgcgtgaa agtgttcaac gcaaattcag
     3901 aagtggttcg tgatgaaatc gtgaacagcc attcctataa aactcaattg ggcgcggggg
     3961 agtacaccac tattctgatc tccatcggag cggatgggat tgaatcagat ccagtgcctt
     4021 taccaggcaa agtggtgatt gaaacagttc agttagccaa acctgtgatt atttttgatg
     4081 aagtggtaga tgcaaaaaat ccaaaagtga aaatcaaacg tttgcctttg tctaaaggca
     4141 tccccacttt gcgttggaag gaagcttcac ttgtcattga tacggtgggg actttggaat
     4201 accgttattt tttcggagag gaatggattc cagtggatgc gttttcctcg aag
//
LOCUS       DATBTE010000397         7870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1761792, whole genome shotgun sequence.
ACCESSION   DATBTE010000397 DATBTE010000000
VERSION     DATBTE010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 7870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7870
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1761792"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="VIG33_15170"
     CDS             complement(<1..1189)
                     /locus_tag="VIG33_15170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LruC domain-containing protein"
                     /protein_id="HEY8272230.1"
                     /translation="MKPSYKWGFPIIIIFVLILAGFQNCAPTSFKAAESVTAEGLGSP
                     SDILPADVLPKDCDLEKRKDGETWLSANGQERELMACILGVGNQFKVYDLQKEYICKD
                     GTASLTGKDVKSYVGVEGLCNLDCGEHKNGELWFMDMGTSNEILQCATSTTATSTVQY
                     RNFAEYKCVNAMASATGVTKKDKISETACPALTFNDQDNQATLAFEDIYPTPLDSDYN
                     DFVTNIKVVETYSSLGELNKIAVEYAAKHIGGGLPHRLITIFDGTVRGRDGWVSNQNS
                     FKSESMFNGTATIKYELYNGNGQLLNSIANLAKDQDFTVFESTKTAATNLQVAKITIT
                     DIEGKLNMLADRKGLSIKRYRTLLYIPDAGNYSKVKRYDIDISDINPSAYDDSGRPLA
                     FFVP"
     gene            complement(1397..1795)
                     /locus_tag="VIG33_15175"
     CDS             complement(1397..1795)
                     /locus_tag="VIG33_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HEY8272231.1"
                     /translation="MVKKKPIIFFDGICHLCNAFVDKVTQLDHKSQFQFAPLQGETAK
                     KSLTLEERTQLETIILFENEHKYLRSDAVLRILTRLGGFYRIFTLGYLLPRFLRDSFY
                     AWMARHRYAWFGQREFCRLPQPHEKERFLP"
     gene            2107..2445
                     /locus_tag="VIG33_15180"
     CDS             2107..2445
                     /locus_tag="VIG33_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272232.1"
                     /translation="MLKTILVTAFISVTAFTAVSLGESVEGTSQVAPSLSLVQEKGGP
                     ITSETLEPDYHRGYYGRYGRYGRYGHHGYYGRYGRYGHHGRYGHYGRHHGFFGHHGRF
                     GHQDSFDNHD"
     gene            2516..3052
                     /locus_tag="VIG33_15185"
     CDS             2516..3052
                     /locus_tag="VIG33_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272233.1"
                     /translation="MKLRKKNEKPWLRIGIVAGLCHLTLVTLGAADIGFSNQHWWGRV
                     INYYGEISGSTFGYGFFAPGVSSQIRAVFDVVESDSRQTTRLLKDDGNREIDLRIGDI
                     IEQFIGEERKDPLEFQRALSASLSGAVFIEHPSAKSVTIRLEKFDPISMEEFRKGLRP
                     SWVSLYSAKFINRKGSSK"
     gene            3049..3972
                     /locus_tag="VIG33_15190"
     CDS             3049..3972
                     /locus_tag="VIG33_15190"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HEY8272234.1"
                     /translation="MMTSAIEQIRLRFTEFMLKPASATPLAILRITVGFTLLLQAFMV
                     SSIIFKIFSSSGYIQGELAQALNNPYLPQIAHLQYLLSPFGANETFCILLVCFSYVIG
                     LFLLIAGWQTRLMSFLVWLLHWILMNSAQSTNYGVDHYAHIFLFYFIWVPCGSAWSID
                     AWKKGDLNPPSSTARIGLRVLQLHLCISYLMSGIEKAQGIQWWNGELLWRALTLPVYR
                     QFDMSWLAYWPRLSMIAGWGSLFLELGYCVLIWPKLTRKIWVAGIIGMHLGIFIFLGL
                     HLFGLFMIFLTACLFGLSAEDSLLIDRVSSK"
     gene            3985..4524
                     /locus_tag="VIG33_15195"
     CDS             3985..4524
                     /locus_tag="VIG33_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272235.1"
                     /translation="MKGIEENTFLRKLKISVLCFLLVIGPVVIPQTLSSPPLDPSVIA
                     PIEQLSKAFAAIAARVKPAVVSVFSEKMIRFRPDEMPFPFGDDLLREFFGRQAPGPQS
                     PGHPREYKIPQLGMGSGMVLDHHGHILTNYHVVQNVDDIKVLFQTIKPLKPKLLEKIS
                     IQMSPSFKSKTPLWKTTLS"
     gene            4449..5477
                     /locus_tag="VIG33_15200"
     CDS             4449..5477
                     /locus_tag="VIG33_15200"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY8272236.1"
                     /translation="MGKDLNTDVAIIQVKDPTMENYPIINWGDSEAMQVGDIVLAIGA
                     PFGLTQTVTHGIISAKGRSDVGISDFEDFLQTDAPINPGNSGGPLVNARGEIIGMNSA
                     IATSIGQSGGVGFAIPSNMIKAMLPKILKGEPILRGELGIGIQNLTEDLARHFGIKDL
                     KGVLVSQVTPNSSAEKSGIKVGDIITNYDGKPVEDARVLRNLVAGSLPGSKVKIDLVR
                     NQRHKTIQVAIGGQSRASAQPRSEGRESQSILNQLGLTVETLTKDLALRLQTKETSGV
                     AILNVDEGSAAAFTGLREGDIIIQANRKPVKNLDDLNKILSQLKNNTVLLLVKRQGAS
                     IFFSIQVG"
     gene            5570..5908
                     /locus_tag="VIG33_15205"
     CDS             5570..5908
                     /locus_tag="VIG33_15205"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY8272237.1"
                     /translation="MKKILMAMIAATSVVVAKAEDKSKSQVVELSVTEKGFEPNSIDV
                     GPGKTVILKITRKTDSTCATEIQIPAKKIKKDLPLNKDVSIDLGKLEKGEIRFGCGMN
                     MMEGGRIIVK"
     gene            complement(5909..6310)
                     /locus_tag="VIG33_15210"
     CDS             complement(5909..6310)
                     /locus_tag="VIG33_15210"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEY8272238.1"
                     /translation="MKYAWKFLILVVLGIGGCNYTNLKRAPGSENLQLNSEPLVPSYE
                     SINKRIFIAACKDCHNSGGSGKRVLLDKVSLLNSPLELVIPGNPDESGLVLSLERNDD
                     KRMPPSKEGYAPLKEEEKAVIRKWIENGAMD"
     gene            complement(6330..6914)
                     /locus_tag="VIG33_15215"
     CDS             complement(6330..6914)
                     /locus_tag="VIG33_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272239.1"
                     /translation="MKKIIIIVCMFMMTVSAFAHPEGPGVSNAKAMELAAHRVDRLVT
                     LGKIEGSFLKQLEKIEIAIVQDQAPIYYKVRISQIPPGQGAPMQLDISFNEEGKALDF
                     QVLSGGVGGPDAGWTGIDAVSLVENALHYILENSGDAKMIPFDKGISSITLSKEMLNG
                     ESVAHTQATSNLTSDKLNIYLKLNGSFISAEMTP"
     gene            7080..7535
                     /locus_tag="VIG33_15220"
     CDS             7080..7535
                     /locus_tag="VIG33_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY8272240.1"
                     /translation="MTDQEKQRAVGVLNKIMELELAGVVRYTHYSLMIYGYNRIPIVS
                     WLKSNANESLAHAHKAGELVTLLGGHPSLKIGPLLETAKHDIGDILKESLEHEKASIA
                     AYFELLKVSEGISVLLEEYARGMIVSEELHLDEVNKMLRKPGDVQPFNL"
     gene            complement(7542..>7870)
                     /locus_tag="VIG33_15225"
     CDS             complement(7542..>7870)
                     /locus_tag="VIG33_15225"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEY8272241.1"
                     /translation="PEMMDLHHEAARVIAQIISGVGFLGAGAILHNKSTHVIGLTTAA
                     WIWFTAAIGVMIGIGHGPAALFTTTTLVIVISAARHLEHKYFGKQHLEPKAKEERIEF
                     PFKKAS"
BASE COUNT         2214 a         1754 c         1674 g         2228 t
ORIGIN      
        1 ccggtacgaa gaatgccaag ggccttccgg aatcatcgta agcggaagga tttatgtcac
       61 tgatatcaat gtcataacgc ttgaccttac tatagttgcc agcatctgga atatagagca
      121 gcgttcggta acgcttgata gaaaggcctt tacgatcggc gagcatattg agttttccct
      181 caatgtccgt gatcgttatt ttcgccacct gaagatttgt tgccgctgtt tttgtgcttt
      241 cgaagacagt aaagtcctgg tctttcgcca ggtttgcaat ggaattcaat aactggccat
      301 tgccattgta aagttcatat ttgattgtcg cggttccatt aaacattgac tcagacttga
      361 atgagttctg gttgctcacc cagccgtctc gcccacgaac ggttccatcg aatatagtaa
      421 tcagtcgatg aggaaggcct cccccgatgt gtttggctgc atattcaacc gcgattttat
      481 tcaactcccc aaggcttgag taggtttcca cgaccttgat attggtcacg aaatcattat
      541 agtcagagtc taaaggcgtc ggatatatat cctcaaaagc caaagtcgct tgattgtctt
      601 gatcgttgaa agttagagct gggcaagcag tttcagagat tttatctttc ttggtaacac
      661 ctgtcgctga tgccatggcg tttacgcatt tgtattccgc aaagtttcga tattgcacag
      721 tggaagtggc agttgttgag gtggcgcact ggagaatttc atttgaagtt cccatgtcca
      781 taaaccaaag ctcaccattt ttgtgctcgc cacaatcaag attgcaaagc ccctcaaccc
      841 caacatatga cttcacgtct ttccccgtga ggctggcagt cccatcctta caaatatatt
      901 ctttttgtaa gtcgtatacc ttgaattgat taccgacccc gagaatgcaa gccatcaatt
      961 cacgttcttg gccatttgcc gacagccagg tttcaccatc ttttctcttt tccaaatcac
     1021 agtcttttgg caagacgtct gctggcaaga tgtcgcttgg actccccagc ccctctgccg
     1081 tcactgactc tgcagcctta aatgaagtgg gtgcgcaatt ttgaaatccc gccaaaatca
     1141 agacgaaaat gataataata ggaaaacccc atttataaga cggtttcata cacattcccc
     1201 cttggacttc tttattctca ccaaatggag aggaaaaata aagaactagt acaaataaaa
     1261 atttgttgtc taaaagatct tcagagaatc accgccaaag catgcttaag catttctaaa
     1321 cacagaccaa aacatgcgtg taattttttg agaatctcat agagagaatt cagccaaaac
     1381 gaaggaccca gtgcctctaa ggaagaaaac gctctttttc atggggctga gggagacgac
     1441 agaactctct ttggccaaac caagcatagc gatggcgagc catccaagcg tagaaggaat
     1501 cccgcaaaaa acgaggcaat aaataaccca aagtgaagat cctataaaag ccacccaatc
     1561 ttgtcaggat cctgagaacg gcatccgagc gcagatattt atgttcattc tcaaataaaa
     1621 taatcgtttc aagctgcgtg cgctcttcaa gcgtcagact ttttttagcg gtctcgccct
     1681 gcagaggggc gaattgaaat tgagatttat gatcaagttg cgtgacttta tcgacgaagg
     1741 cattgcaaag atgacagatt ccatcaaaaa aaattatagg ttttttcttt accaaaagac
     1801 ccctctctcc atctcttcgt gacgaataag gcccctcaaa tgagccattc ttgtttataa
     1861 tactagttca gaacaatcgg caatgaactt aaaaggatag caacattttg atgaatgata
     1921 aatggcacag ttctatcttt gatttctttt ttttgaaaga tgaataaaaa gtaacaggat
     1981 cgtcggtttt ttaacggagt cgggcgaata ttacagaaat cgtgtttgtg agttaaaaaa
     2041 atgaatcgcc agtcttacac catgtcggtg aagtcgtcga gtggttcaga tcataggaga
     2101 ggtgtcatgt taaaaacaat tttggttaca gcatttattt ctgtcacagc ctttactgct
     2161 gtcagtcttg gcgaatctgt ggaaggaact tcacaagtcg ctccttcact gtctcttgtt
     2221 caagaaaaag ggggtccaat cacctccgag accctagagc ctgattatca tcgtggttac
     2281 tatggacgtt atggacgtta tggccgctac ggtcaccatg gttactatgg acgatatggc
     2341 cgctacggtc accatggtcg ctacggccat tacggccgtc atcatggatt ctttggccat
     2401 cacggtcgtt tcggacacca agacagtttt gacaatcatg actaactaaa aaaattaggt
     2461 caactttgaa gctctctttt tagttaacga gggagctttt tttcttagga ctcttatgaa
     2521 acttcgaaaa aaaaatgaaa aaccttggct aagaataggc atcgttgcag gactttgcca
     2581 tttaacgctt gtgaccctgg gtgcggctga tattggattt tctaatcaac attggtgggg
     2641 gcgtgtgatt aattattacg gtgaaatttc tggatccact ttcggatatg gattttttgc
     2701 tccaggagtc agctcccaaa tacgcgccgt ctttgacgtc gtcgaaagtg actcacgaca
     2761 aaccactcga cttttaaaag atgatggcaa tcgcgagatc gatctgcgaa ttggggatat
     2821 tatcgaacag ttcattggtg aagaacgcaa ggaccctttg gaattccagc gtgccctttc
     2881 agcctctctc tcgggagccg tcttcattga gcatcccagc gccaaatctg taacgattcg
     2941 cttggaaaaa ttcgacccaa tttccatgga ggaatttcgt aaaggtctta ggccttcttg
     3001 ggtttctctt tattccgcta aatttataaa ccgtaaaggt tcttcaaaat gatgacttct
     3061 gcgattgaac agatccgact tcgtttcacc gaatttatgc ttaaaccagc ctcagcgact
     3121 ccccttgcta ttctgcgcat caccgtggga ttcacgctgc tcttacaagc gtttatggta
     3181 tcctcgatca tttttaaaat tttttccagc tccggctata tccagggtga gcttgcccaa
     3241 gctctcaaca atccctatct gccgcaaatc gctcacttgc agtatctatt aagtcctttt
     3301 ggagccaatg aaacattttg tatcttgttg gtctgtttct cctatgtcat tggtcttttc
     3361 ttgttgatag cgggttggca aacacgactt atgtcttttc ttgtatggct attgcattgg
     3421 attcttatga acagcgcgca atcaacaaac tatggagtgg atcactatgc tcatattttt
     3481 ctattttatt ttatctgggt tccctgcgga tccgcttggt ctatagacgc ttggaaaaaa
     3541 ggagacttga atcctcccag ttctactgcc cggattggtt tgcgggtttt gcaattgcat
     3601 ctctgtatct cttatttgat gagtggaatt gaaaaagctc aaggcattca atggtggaac
     3661 ggagaactct tgtggcgagc cctgacgctg ccggtatata gacaatttga tatgagttgg
     3721 ctggcttatt ggcctcgttt atccatgatt gcaggttggg gcagtctttt tctggaactt
     3781 ggctattgtg ttttaatttg gccaaaactc acccgtaaaa tttgggtggc gggaatcatc
     3841 ggaatgcatc ttggcatctt tatcttcctg ggtctgcatt tgttcggtct atttatgatt
     3901 ttcttaaccg catgcttatt tggtctgtcc gcagaagact cgcttcttat cgatagagtc
     3961 tcatcaaaat aaaaaaggaa agcaatgaaa gggatcgaag aaaacacctt cttgaggaaa
     4021 ttaaaaattt cggttctttg ctttcttctt gttatcggcc cagtagtcat acctcagact
     4081 ctttccagcc cgcccctgga cccttccgtc atcgcaccga ttgagcaact cagcaaagcc
     4141 tttgccgcca tcgccgcccg ggtaaaaccc gctgtcgtca gtgtcttttc agaaaagatg
     4201 attcgcttcc gtccagatga aatgcctttt cctttcgggg acgacttgct acgggaattt
     4261 ttcggccgac aagctccggg tccacaaagt ccgggtcatc cgcgagaata caaaattcct
     4321 caactgggca tgggctccgg aatggttctt gatcatcatg ggcacattct caccaattat
     4381 catgtcgtgc aaaacgttga cgacatcaaa gtcttattcc aaaccataaa acccttaaag
     4441 ccaaagttat tggaaaagat ctcaatacag atgtcgccat cattcaagtc aaagacccca
     4501 ctatggaaaa ctaccctatc ataaattggg gcgactcgga agcaatgcaa gtcggagata
     4561 tcgtcctagc gatcggcgct cccttcggac tgacccaaac ggtcacccat ggcatcatca
     4621 gcgcgaaggg ccgctctgat gtggggattt cggactttga agacttttta caaactgatg
     4681 cccccattaa tcctggaaat tcgggaggcc ctctggtcaa tgcccgaggt gaaatcatag
     4741 gaatgaacag cgcgattgcc acaagcattg gccagtctgg aggcgtcgga ttcgccattc
     4801 ccagcaatat gatcaaagcc atgcttccga aaatacttaa aggagaaccc atccttcgcg
     4861 gagaattggg tattggcatt caaaatttaa ccgaagattt agctcgccat tttggcataa
     4921 aagacctgaa aggtgtttta gtctctcagg tgactcccaa ctccagtgct gagaagtctg
     4981 gaatcaaagt gggagacatc atcaccaact atgacggaaa acctgtggaa gacgcgcggg
     5041 tcttacgcaa ccttgtggct ggatcattac caggcagcaa agtaaaaatt gatttagtga
     5101 gaaatcaaag acataagaca attcaagttg ccattggcgg acaaagcagg gcttcagctc
     5161 agccccgttc ggaagggaga gaatctcagt ctattcttaa tcagcttggt ttaacggtcg
     5221 aaactctcac taaagattta gcacttcgtc tgcagacgaa agaaacatca ggagtcgcca
     5281 ttttaaacgt tgatgaagga agtgccgcag ccttcaccgg acttcgcgag ggagatatca
     5341 ttattcaagc caaccgcaag ccagtgaaaa acctcgatga ccttaataaa atactttctc
     5401 aactcaaaaa taataccgtt ttgctgttgg tgaagcgcca aggtgccagc atatttttca
     5461 gtatccaggt cggatgagat tttacttgag cttttgagat atccagacga ttaattatac
     5521 aattccattc ttaaagcacg tgacggttgt ccggcactca aaggagttta tgaaaaaaat
     5581 attaatggcc atgattgcgg caacttccgt tgtcgtcgcc aaagctgaag ataaaagcaa
     5641 atcccaagtt gtagaattgt ctgtcaccga aaagggattt gagccaaact caatagatgt
     5701 aggtccggga aaaacagtga tcttaaaaat tactagaaaa accgattcca cttgcgccac
     5761 agagattcaa attcccgcaa agaaaattaa aaaagacctg cctttgaata aagacgtgag
     5821 catcgatttg ggaaaacttg aaaaaggtga gattcgtttc ggctgtggca tgaacatgat
     5881 ggaaggcgga agaattatag taaaataatt aatccatggc gccgttttca atccattttc
     5941 gaattaccgc tttttcttct tcctttaagg gggcataccc ttcttttgac ggcggcattc
     6001 ttttgtcgtc atttctttca agagacagca ctaagcccga ctcatcagga ttaccaggaa
     6061 tgacaagctc tagaggagaa ttcagcagcg aaaccttatc gagaagaacc ctttttccgg
     6121 aaccaccgga gttgtgacaa tctttacatg cagctatgaa aatgcgctta ttgatggact
     6181 cgtagcttgg aacaagcggt tcagaattaa gctgcagatt ctcagatcct ggggctcttt
     6241 tgaggttggt ataattgcaa cctcctatgc ctaagaccac gagaataaga aacttccaag
     6301 cgtatttcat agttttcctg ctctgtaagc tatggagtca tttcagcaga gatgaaggac
     6361 ccattgagtt ttaaataaat attcaattta tctgaggtca gatttgaggt ggcttgagta
     6421 tgagccacac tctctccatt caacatttct ttggaaagag taatacttga aatgccttta
     6481 tcaaaaggaa tcattttagc atcaccgctg ttctcaagaa tataatgaag cgcattttcc
     6541 actaatgaaa cggcatctat tccggtccaa cccgcgtcag gtcccccaac tcctccagaa
     6601 agcacttgga aatcaagggc ttttccctcc tcattgaaag aaatatctaa ctgcataggc
     6661 gcaccctgcc ccgggggaat ttgagagatt ctcactttgt aatagatagg cgcttgatcc
     6721 tgcacgatcg ctatttcaat tttttccagt tgttttaaaa atgacccctc aatctttccc
     6781 agagtgacca agcgatccac tctatgagca gccaattcca tcgccttcgc attgctgaca
     6841 ccaggccctt ctggatgtgc aaaagcagaa accgtcatca tgaacataca aacaatgata
     6901 ataatttttt tcataaaacc tctctaaggg gaaataacat ctaaaacaaa ttggagcaac
     6961 agatgcaatc cgtcatttca tctcctaaaa aaaagatttt agagcactat cctcttcaat
     7021 aagcttgtat tttttttgac cgtgggtagc atattttctt caacaaagga gatatcccca
     7081 tgactgatca agaaaagcag cgagctgtag gtgttcttaa taaaattatg gagcttgagt
     7141 tagcgggagt ggtccgctac acgcattatt ccttgatgat ttatggatat aatcgcatcc
     7201 ccattgtttc ttggctcaag agcaatgcga atgaaagcct cgctcatgca cataaagcag
     7261 gtgaactcgt gactttactg ggtggacacc cttcgcttaa gatcggtccc ttattagaaa
     7321 ccgcgaaaca tgacattggt gatattttaa aagaaagttt agaacatgaa aaggcatcga
     7381 tcgctgcgta ctttgaactg ctgaaagtct cagaaggaat ttcagttttg ttagaggaat
     7441 acgcaagagg tatgattgtc agcgaagaat tgcatctcga tgaagtgaat aaaatgctgc
     7501 gaaaaccagg ggatgttcag ccctttaact tataatattc attagctcgc ttttttgaag
     7561 ggaaattcaa tcctctcttc tttggctttt ggttcaagat gttgtttacc aaaatattta
     7621 tgttctaagt ggcgggctgc ggaaataaca atgactaatg ttgtggtggt aaaaagtgcc
     7681 gcaggcccgt gtccaattcc aatcataact ccaatggccg ctgtaaacca aatccaagcg
     7741 gccgtagtca gtccaatcac atgagtggat ttattatgga gaatggcacc cgccccgaga
     7801 aagccaacgc ccgaaataat ctgagcaatc actcgagcgg cctcgtgatg caaatccatc
     7861 atttcaggaa
//
LOCUS       DATBTE010000398        19968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1900121, whole genome shotgun sequence.
ACCESSION   DATBTE010000398 DATBTE010000000
VERSION     DATBTE010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 19968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19968
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1900121"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VIG33_15230"
     CDS             <1..261
                     /locus_tag="VIG33_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail-specific protease"
                     /protein_id="HEY8272242.1"
                     /translation="EDFKKIEDELKKAKARGKLIKVSEVLKDKTEIKTKKEKEKAVRY
                     GNKDVKNKEYLKRADVNEATNILLDLMNVEESQPTGKQASSK"
     gene            424..1524
                     /locus_tag="VIG33_15235"
     CDS             424..1524
                     /locus_tag="VIG33_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY8272243.1"
                     /translation="MSKKIVNSSAKAKAVPLGKPIPKATIKAAPKKSKNDFGGLSKDL
                     LLQFHNLMVKSRVLEERLIKIYKAGEGYFWIGGPGEEAFGVSLGLLARKGEGLKHDWF
                     HLHYRCTPTMVALGMPMIDAARLMMNRSTDASTGGRNFAGHYCFPKWNVAPVTSPIEV
                     QYPLACGTAHAQKRAGHNSISIVTGGDAGTAEGDFATCLIWASRKGQELPVLITVQNN
                     GFGISTPYEGQHGETQIADRAKAFNIRARVINGNDPVETYLALKEEMEYIRKTGKPSF
                     VEVKVSRLYGHSSADGANRKVELEDPVFSFEKKLIEAGILSKVEAEKIWADYEAEGVA
                     AQIQARGEPSPTPESVWENVYVNSENADWRKF"
     gene            1524..2579
                     /locus_tag="VIG33_15240"
     CDS             1524..2579
                     /locus_tag="VIG33_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8272244.1"
                     /translation="MASIAQAIRMALHYGEKHLNVKDVFGQDVGAPLGGVFTATQGLK
                     TAWNTPLDERGIIGMAMGIAMTGDRCIAEIQFADYIFNTMDLLKIAGNTLWCTNGQVK
                     LPMVVMTPVGAGIFGSVYHSHSFDAWASRLPGWKIVMPSNPLDAYGLMLSAIQDPNPV
                     LYLKSKALMRHKGEELIPGEPSDEKELKAMIDKPVQNSVGWKPRWPDLEEYLVPIGVG
                     KITHPGEHITVVTYSRMVHVCNEVAYKLSQEGISVEVIDLRSIYPYDWQIIKASVEKT
                     GRVLFVNEDTEVTNFGEHLSYRTVQECFYHLLARPRVLAGKNLPGIGLAPNLEKNSVP
                     QVADVELAIREVVAEIP"
     gene            2579..3421
                     /locus_tag="VIG33_15245"
     CDS             2579..3421
                     /locus_tag="VIG33_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8272245.1"
                     /translation="MTKKAVTHRKKVRRRVNKKSTLTVFDKYDLYRRAVQSAESDVEF
                     LRDTYKELKGKNARTFREDFCGTFALSTEWIKLNPKNQSIGIDLDPEPMAYGKTQYLS
                     KLRPEQQKRMQLLEGNVLHPDLPEADIVAAMNFSYFCFKSREVMKLYFTSVYKSLGKD
                     SIFLVDCFGGSQCHAPIEDTINHDGFKYHWDQSSFDPVTNEAVFYIHFRIGGKKIEKV
                     FTYDWRMWSIPELREIMAEVGFKKTHVYWEGTAKDGSGNGVFSRTEQGEACDSWIAYI
                     VAEK"
     gene            complement(3471..4388)
                     /locus_tag="VIG33_15250"
     CDS             complement(3471..4388)
                     /locus_tag="VIG33_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272246.1"
                     /translation="MLRFIKLMALTGLLVTPLFSRAKDELFLRSAELKCVADGVEIAT
                     GPGGSLGLRPSEKYLALLGFAVQRNYKSVNSSTGGFNRELYLQRVIQILQADGFCVAN
                     KLDGGGAPTSQVGYGDVVRDKSNAVGRYLIYAVTGLKSKEDKNGDTQSVLTADQVNEV
                     IKYFGFQNETYIQNLFRHDNWSSLEPDARQKLFSDNVESTSATAPEDRTSVSGMYSSN
                     EYGEGLKDCFAQMKQMQSKSTIFNFKNLLKGRSSGGRKFCETVAKECQLESTTFCSIS
                     APTFKTEGNGSTPGSTGEGSIFDKLKGTQ"
     gene            complement(4507..5031)
                     /gene="pyrE"
                     /locus_tag="VIG33_15255"
     CDS             complement(4507..5031)
                     /gene="pyrE"
                     /locus_tag="VIG33_15255"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEY8272247.1"
                     /translation="MNKTELAKKIYEVAHLTGEFKLRSGQISHEYFDKYRFEARPDLL
                     NEIALQMRALIPPGTQAFAALEMGGIPIATALSLITGIPCVFVRKEAKEYGTCKFAEG
                     LEIKGLKLCVIEDVVTTGGQVILSTADLRSMGAQVADVLCVIHRGGGEEKLKEVGLKL
                     QALFNMDELKAHLK"
     gene            complement(5070..6107)
                     /locus_tag="VIG33_15260"
     CDS             complement(5070..6107)
                     /locus_tag="VIG33_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY8272248.1"
                     /translation="MASIIFLFCLTISISFICSLLEAVILSVSPAYVAVAVQNRKRSG
                     LLLQHLKENLDRPLSAILTLNTISHTLGSSAIAYKVQQLYGEDLVTLSSFILTFGILI
                     ISEVLPKSIGAAHWKVIAPVAAYVIQTMIFVLYPLVSFSQFVGKLVEKPDTQQVTREE
                     MIMTAELGANEGTLKTKESNMIKNLLMLDKIYVSDIMTPRSVFFALEADITVDEVVNK
                     YVPLRFSRIPVYRDSLDNIIGITHRYKILEASSDDQHTRKISDLVTPIASVSERLTVA
                     QVLDFFIKEKEHLALALDEYGVIAGLVTLEDAVETLLGVEIVDEFDNIEDMRKYALDQ
                     WQIRKKQLRRS"
     gene            6316..7110
                     /locus_tag="VIG33_15265"
     CDS             6316..7110
                     /locus_tag="VIG33_15265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY8272249.1"
                     /translation="MKNMFISFFIFLGGLVVKAEVKTEVVEYKQDGTTLEGFLAYDPS
                     FKGPHSAILIVHEWTGLGDYVKGRAKELAAKGYVAFALDIYGKGVRPALPKEAAKEAG
                     KYKENRKLMRERAKAGYDFIKAQPFVDSSKIIAMGYCFGGTVALEMGRSGLPLAGVVS
                     FHGGLATPTPQDAKNFKAKLLVLHGAIDPYTKDEVPGFQKEMDEARVDYQLISYGGTV
                     HAFTNPQAGNDIKTGAAYNALSDRRSMQAFMGFLNEVAPIGKAATH"
     gene            7241..7705
                     /locus_tag="VIG33_15270"
     CDS             7241..7705
                     /locus_tag="VIG33_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272250.1"
                     /translation="MERKLNSFLALGLIFGTFSSTQAAPIGSPQDYILQTKYLCQANG
                     IVVRNKQKLVSNGRGVDEVQFVANAGKNQALMFGGKSEYMILPQVTNGTFTKSLNVLS
                     RDRSGTYKVVQEVDMGETQKTKTNIGLSVKDGQGEARPVSCRIEMEWLKRAK"
     gene            complement(7756..8406)
                     /locus_tag="VIG33_15275"
     CDS             complement(7756..8406)
                     /locus_tag="VIG33_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY8272251.1"
                     /translation="MKKQIIIFGTAAAMLSACASGDNKNAAKDAGIGTAIGAVAGAVI
                     GHQTGKRNEGAMIGAALGAGLGGAIGHRLDKQQKELEKIAETKRTEQGLVTKLKSDIL
                     FDSGKADLKAAAKTNITELAGIMKKYPENVLTIKGYTDSTGTDKVNKPLSEKRAKSVR
                     DALVAGGIPDSTISFVGMGSENPVDQGKSKDALSRNRRVEIEITVDESKVPKQAQN"
     gene            8596..9792
                     /locus_tag="VIG33_15280"
     CDS             8596..9792
                     /locus_tag="VIG33_15280"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY8272252.1"
                     /translation="MRDMFGDDDSQNKKDDFGAMFEQSIQGYTKSYGKGDKVTAEVVT
                     LGRDEIFVNVGGRDGLVPRSEFVDAEGGLKVQIGDQVTLYVIKSQEGLLVLSAKASSK
                     AMSDSLEDAFDFETPVEGRVTEVVNGGFRVNVMGKSAFCPLSQMDSKPITEPESYIDR
                     KFEFQITKFEQKGRNIVVSRRRILDMEKLENQGSFMDTHKAGDLLQGRVSRIENYGAF
                     VELVSGIDGLVHISEVSWSRLAHPSEALAVGQEVSVKILKIEEDALGRLKISLSCKQA
                     EEDPWMTAYKDFPVGSVHTAKILSKERFGFLMEIKPGIVGLLPKSAFREVADEREMEK
                     KNSGQTLKVQIQNVNTVEKKIALSFPKDQEDTSWQGFTGSGTVSGKGLGTLADQFKNL
                     SLTKKK"
     gene            complement(9917..11059)
                     /locus_tag="VIG33_15285"
     CDS             complement(9917..11059)
                     /locus_tag="VIG33_15285"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY8272253.1"
                     /translation="MPLIQSQKRKSPKWKVGWRHEVNKKRSHKKPLHYLDTPLHVNRI
                     EQATLEALMEACCKNLEETRKAPAMMAKLLKKSQLDSWDLLAPSPLGDTGKQTSFNEA
                     ICLIREAFADVDPQMADFVDLMVKNQWIEGRVLPNKTTGAYCTNFPKTKEPRVYVTYL
                     GSDQDVSTLAHELGHGFHAWVMRDLPRAQINYPMTLAETASVFAETALREILLKKAST
                     HEEKLDCAWSNLESSSAFLINIPARFDFEKSFYDKRAERALNPQELSELMNKAWTKWY
                     GPVLSKNDDLFWAHKLHFSIAGDQSFYNFPYTFGYLFSLSLYARRKELGSDFMKAYLG
                     ILRDTGRMTAEDLIQKHLDEDIRKPQFWQKSIDLVLQKLNRIAALT"
     gene            complement(10998..11279)
                     /locus_tag="VIG33_15290"
     CDS             complement(10998..11279)
                     /locus_tag="VIG33_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272254.1"
                     /translation="MTPPAWNLESQHPSVDSPAFQEDFAFVSNAIITIEKKNSSKDIP
                     TLQDLFILCEKAETTLGNLFTYVHCILSVDATDPIAKAKESEVEGRMAP"
     gene            complement(11348..13456)
                     /gene="fusA"
                     /locus_tag="VIG33_15295"
     CDS             complement(11348..13456)
                     /gene="fusA"
                     /locus_tag="VIG33_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY8272255.1"
                     /translation="MSKKWNIDMVRNIGISAHIDSGKTTTSERILFYGGRIHAIHEVK
                     GKDGVGATMDSMDLEREKGITIQSAATQVHWKDYTINLIDTPGHVDFTVEVERSLRVL
                     DGAILLLCGVAGVQSQSITVDRQMKRYSVPRLAFVNKLDRQGANPFRVTDALIDKLKL
                     NAVMIQIPIGLEDQHRGHVDLTDMKAYINEGDNGETVNVTDIPADMVEQAKEYRQIMI
                     GKLADIDPKIEEKFLMEEEPTTEEIRAAIRAGTISLKLVPVLCGSAFKNKGVQRLMDA
                     VTYYLPSPAEKKEHALDLTKNEEKFELFSDNSKPLVALAFKLQETQFGQLTYMRVYQG
                     KMSKGDFIVNQTNKKSVKIPRLVRMHSDKMEDIDTSYAGDIVALFGIDCASGDTFCDD
                     KIEASMQSMHVPDAVISLAVAPKHKEGANNFTKALQKFRKEDPTFRVHRDDESNETII
                     SGMGELHLEIYVERMKREFNCEVIVGQPQVAYRETIAVEAAYDYTHKKQTGGSGQYAK
                     IVGSIRPLPPQEDGSVFKFTNSVVGGRIPREFIPAVEEGFKEQTVKGPLIGFPIVGVE
                     VELTDGAYHDVDSSYMAFKIAAMAALRESYPSAKPTVLEPIMKLETTVPDEYQGSAVG
                     QINQRRGVIVGTTSFEGNCVIEAEVPLTEMFGYSTDLRSGTKGKGEFSMEFAKYQAAP
                     RNIQEELVKKYQAKRAAEQK"
     gene            13598..15052
                     /locus_tag="VIG33_15300"
     CDS             13598..15052
                     /locus_tag="VIG33_15300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEY8272256.1"
                     /translation="MNFLQILTENFPYKHFRGEQENILKRVMARQDTLALMPTGMGKS
                     LCFQFPAKVQKDLVLVISPLIALMEDQVQKAQKLGISATYLSSTVSRADRESRQERLA
                     SGDYQLFYVTPERFRKEEFLSAVKTRQIFCLAIDEAHCISQWGHDFRPDYSRLGEIRK
                     LLGKPPILALTATATPAVQKDILKNLNMEENGEIIFGGLERPNLELNVHDVYGIDEKI
                     RAIIGLRHQALGAGIIYCSLIQTLKKISGDLRRLGVEHLVYHGDLPPPDRKRNLRSFT
                     SQENPLMVATPAFGLGIDKENVRLLIHAEIPGSIEAYYQEVGRAGRDGKPSQCHLLYD
                     QEDVSIQMEFLKWSHPEPEFIAQVYKLIDKNRGPLDQDRFDYLREQMSFKNRRDFRVE
                     SAVNILERWGCLASAETPFPFVTVQEPTKEMFETENSPYLLKNQNQKLYDMVRYATSQ
                     EECRLLQVYEYFGYEGGQKCGHCDICGVEIQAKS"
     gene            15245..15610
                     /locus_tag="VIG33_15305"
     CDS             15245..15610
                     /locus_tag="VIG33_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY8272257.1"
                     /translation="MGKKIYVGNLSYSLNDQSLVDVFSEFGNVESARIVTDRESGRSK
                     GFAFVEMSSDDEAQTAIAKLNGVELEGRALNVSEAKPMAPREGGGGRGGFGGGGRSGG
                     GGRGGFGGGGRGGGGGGRY"
     gene            complement(15676..16839)
                     /gene="chrA"
                     /locus_tag="VIG33_15310"
     CDS             complement(15676..16839)
                     /gene="chrA"
                     /locus_tag="VIG33_15310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEY8272258.1"
                     /translation="MTETKPPPLYEIATTFLKLGSTAFGGPAAHIAMMEENLVRRKKW
                     LSHEEFLDLIGATNLIPGPNSTEMAIHIGYKMGGWRGLFLGGICFIFPAFLIVWLLAW
                     SYVKFSSLPQFQFLFLSIKPVILAVIAQAIWSLSKNAIKDKILFGLCAISIFLYGRGI
                     NELIILAAIAITNLFLRQKPPGAGKISLWFLAMPMFLIGLPSWASLNENHRIPVENVF
                     VYFTKVGSVLFGSGYVLIAFLKNDLVDQYQWLNQQQLLDAIAVGQFTPGPVFTTATFI
                     GYLISGHGGAIAATCGIFAPAFFFVAITAPLISRLRKSKWTSPLLDGLNVASLSLMMG
                     AGILLAPESLQSIYEIIIFLLSLWLLIGYKVNSAWLILAAGILGFLKMNLGWV"
     gene            complement(16854..17543)
                     /locus_tag="VIG33_15315"
     CDS             complement(16854..17543)
                     /locus_tag="VIG33_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY8272259.1"
                     /translation="MSKINLKQKFNPENPFSLLMGSSISYHEAQEHSILVDIWLGFNT
                     EEIEKTLFKNHTLQKEHPPVQAWIGLPVQAMQTPYTEIHTILNILNPQPHESVIDLGC
                     GYGRMAHVLGRHFPQVHFTGYELIFERVQEGNRILQKFNYPFVNLETQDLSSPDFTPR
                     LADYYFIFDFGSRSAIAKTLEDLKIIALERSITVVARGRGIRHWIYQDHPWLYAINEP
                     KAFEHFTIFRS"
     gene            17694..17957
                     /locus_tag="VIG33_15320"
     CDS             17694..17957
                     /locus_tag="VIG33_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272260.1"
                     /translation="MKSLVAIFVLLGSSGAFAQGKESRPCQQIKSACEAAGFIKGGHK
                     EKKGLYKDCLEPIMAGQSVAGVMIGADLITACKDNRQAHNKVK"
     gene            complement(18012..18329)
                     /locus_tag="VIG33_15325"
     CDS             complement(18012..18329)
                     /locus_tag="VIG33_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272261.1"
                     /translation="MKSIFMGIILLFSSLFNSLALAQDLNFQEYRKFLMSGTQSVGTK
                     MAIQTTCTTSTGQTYRVGEKGYDTCLNTVKDQFNKKHLASEKSSTAPEATAGASTTIH
                     FGN"
     gene            18481..19125
                     /locus_tag="VIG33_15330"
     CDS             18481..19125
                     /locus_tag="VIG33_15330"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEY8272262.1"
                     /translation="MKKYLFTGLVLTNVFLACGRVPKLGPETDTKLATASEEDCGFVQ
                     NIYGQRVSWKQSFPVKIYIDPSFPVEYNPVLTAAAQKWESVLGKTLFVFERLKQTSTP
                     GKDNQNVLYWLTSWAESDKTLEAVSSLSWTNNQLVETDIKIDAQYYSYYVSTPVSNTE
                     VHLESLLVHELGHILGLKHVSNSIPSVMLQVLDYLLKRDTPTAEDQANLKCEYN"
     gene            19133..19801
                     /locus_tag="VIG33_15335"
     CDS             19133..19801
                     /locus_tag="VIG33_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272263.1"
                     /translation="MKTSKSRSLVGLVLLLIGGLYLGDRLFLQKRGIASLSGGIMSSN
                     DVNYFDLSELSGKDFQRAFKVALAQGLQVQKSLQGVGLSLGLFLVQSGNGSKVYACEK
                     YPNIEVTLKAEGVAHSGNIPTLIVRGPCLVSEDGHKIQAFALPLKDLYKNLRENPMWR
                     VPLGNRGDSLLLSAQYLYNEWPTNWNVVQVKLYSDQESLEIDGYEMISLLDQPLTLDF
                     SEGE"
BASE COUNT         5584 a         4080 c         4692 g         5612 t
ORIGIN      
        1 gaagacttta agaaaataga agatgaatta aaaaaggcaa aagcccgtgg caaacttatt
       61 aaagtcagcg aagtcctgaa agataagacc gaaatcaaaa caaaaaagga aaaagagaag
      121 gcggttcgtt acggaaacaa agatgtcaaa aataaagagt atttaaaacg tgctgacgtc
      181 aatgaagcca ccaatatcct tttggatttg atgaatgttg aagaatcaca gccgaccggt
      241 aaacaggcga gttctaaata accgagagag aattagagaa ataaagagac caagaaaatg
      301 agaaagctcc aggaaactgg ggcttttttg tgtttaaatt acccattgcg tcaatataga
      361 tttgtacgcg acaaaaagag atgttaccgc ttagataatc ctactttttg gaggcttatc
      421 taaatgtcga aaaagatcgt aaattcaagt gccaaagcta aagcggtccc gttggggaag
      481 ccaataccaa aggcgacaat aaaggccgcg cctaaaaaat ctaaaaatga ttttggcggc
      541 ttatctaaag atttattact tcagtttcat aacctcatgg tgaaatcgcg agttcttgag
      601 gagcgtctca ttaaaattta caaagccgga gaaggctatt tctggatcgg ggggcccgga
      661 gaagaagcct tcggagttag cttgggactt ctggcgcgaa aaggcgaggg tttgaagcat
      721 gactggtttc acttgcacta tcgttgcact cccaccatgg tcgccctcgg gatgccgatg
      781 atcgatgcgg ctcgtctgat gatgaatcgc tctacggatg catccaccgg tggacgcaac
      841 tttgcgggac actattgctt tcctaaatgg aatgtagctc ctgtcacttc tcctatcgaa
      901 gtccagtatc cgttggcatg tgggacagcc catgcacaaa aacgtgcagg tcacaactcc
      961 atcagtattg tcaccggtgg tgatgctggg acggcggaag gtgattttgc gacttgcctg
     1021 atctgggcct cacgcaaagg tcaagagttg cctgttttaa ttacagttca aaacaatggt
     1081 ttcggaattt ccactcccta tgaaggacag catggggaaa ctcaaatcgc ggatcgagcc
     1141 aaggccttta atattcgcgc acgagtgatc aatggcaatg atccggtcga aacttatttg
     1201 gcattgaaag aggaaatgga gtacatccgt aagactggaa aaccctcttt tgtcgaagtg
     1261 aaagtgtctc gtttatacgg tcactcttca gcggatggcg caaaccgtaa agtcgaattg
     1321 gaagatccag ttttctcgtt tgaaaagaaa ttgatagagg caggaatttt gagcaaagtg
     1381 gaagcagaaa aaatttgggc agactacgaa gctgaaggcg tcgccgctca gattcaagct
     1441 cgtggcgagc ccagtcccac cccagagtcc gtttgggaaa atgtttatgt taacagcgaa
     1501 aatgcggatt ggagaaaatt ctaatggcaa gtattgctca ggccatcaga atggcccttc
     1561 actatggtga aaagcactta aatgtaaaag atgtttttgg acaagacgtg ggtgctcctc
     1621 tgggaggagt atttaccgcc actcaaggat tgaaaacggc ttggaatact ccgctggatg
     1681 agcgcggaat cattggcatg gctatgggaa tcgccatgac aggtgatcgt tgcatagctg
     1741 aaattcaatt cgctgactat atttttaata ccatggattt gcttaaaatt gcgggcaata
     1801 ctttgtggtg cacgaacggt caagtgaaat tgccaatggt ggtgatgact cctgtgggtg
     1861 ctggaatctt tggctcggtt tatcattcgc attcttttga tgcttgggct tctcgtttgc
     1921 cgggttggaa gattgtgatg ccttccaatc ctctggatgc ttatggcttg atgttgtcag
     1981 cgatccaaga tccaaatcct gtcttgtatt taaaatccaa agctttgatg cgccacaaag
     2041 gcgaggaatt gattcccggc gagccttcag acgagaaaga gcttaaggca atgatcgata
     2101 aaccagtgca gaactcggtg ggttggaagc ctcgctggcc ggaccttgaa gaatatctgg
     2161 ttccgattgg cgtaggtaaa atcacccacc cgggtgaaca tatcacggtt gtcacttaca
     2221 gccgtatggt ccatgtctgc aacgaagtgg catataagtt atcccaggaa ggcatttcgg
     2281 tggaggtcat tgatctgcgc tctatttatc cttatgactg gcaaataatt aaagcctcgg
     2341 ttgagaaaac cggacgtgtt ctttttgtga atgaagatac cgaggttaca aatttcggag
     2401 agcatttgtc ttatagaaca gttcaagaat gcttttatca cttattggcg cgcccgcgag
     2461 ttttggctgg aaaaaatctg ccgggtattg gacttgctcc gaatcttgag aagaattccg
     2521 tgccccaagt agccgatgtt gaactggcaa ttcgtgaagt tgtagcggag atcccataat
     2581 gacaaagaag gcagtgactc acagaaaaaa agttcgtcgt cgtgtaaata aaaaaagtac
     2641 tcttacggtc ttcgataaat atgatctgta tcgtcgtgcg gttcaatccg cagaatcgga
     2701 tgtggagttc cttcgcgaca cctacaaaga gcttaaagga aaaaatgccc gcactttccg
     2761 cgaagacttt tgtggaactt tcgcactgtc gacggaatgg attaagctta atccaaaaaa
     2821 tcaatccatc ggcatcgatt tggatccaga gcccatggct tacgggaaaa ctcaatatct
     2881 ttcaaagctt cgtcctgagc agcaaaagcg catgcagctt ttagagggca atgttctgca
     2941 tccggattta ccggaagcgg atatcgttgc cgctatgaac ttttcatact tctgttttaa
     3001 atctcgcgaa gtgatgaagc tttattttac cagcgtttat aaaagcctcg gaaaagacag
     3061 tatcttttta gtagattgct ttgggggttc gcaatgtcac gcgcctatcg aagacacgat
     3121 aaatcatgat ggttttaaat accattggga tcaaagcagc tttgatccgg tgacgaatga
     3181 agctgttttc tatattcact tccgcatcgg tggaaagaaa atcgaaaaag ttttcaccta
     3241 tgattggcga atgtggtcaa tccctgaatt acgcgagatt atggcggaag tcggatttaa
     3301 aaagacccac gtttattggg aaggcacagc caaagacggt tccggaaacg gagttttcag
     3361 ccgcacggag cagggagaag cctgcgatag ttggattgct tacatcgttg cggaaaaata
     3421 aaaaaggagt tcgaaagaac tcctttttta tttctgaatg tgggagtttt ttattgagtt
     3481 cctttaagct tatcaaaaat actgccctcg ccagttgagc caggagttga tccattgcct
     3541 tctgttttaa aggtaggtgc actgatggag cagaaagtag tactctccaa ttgacattcc
     3601 tttgcaacag tttcacaaaa tttgcggccc ccagagcttc ggccctttag aagattttta
     3661 aaattgaaaa tagtggattt gctttgcatc tgcttcattt gagcgaagca atccttgaga
     3721 ccctcaccgt attcattgga gctatacatg ccacttacgc tggtgcggtc ttcaggggcc
     3781 gttgcggaag tgctttccac attgtccgag aagagtttct gtcttgcatc cggttcaagg
     3841 ctagaccagt tatcatgtct gaagagattt tgaatataag tttcattttg aaatccgaaa
     3901 tactttatta cttcatttac ctgatcagca gtcagaacac tttgagtatc tccatttttg
     3961 tcctctttgc tttttagtcc ggtcactgca taaataagat agcggccgac agcgttgctt
     4021 ttatcacgaa ccacatcacc ataaccaact tgactggtgg gagctccgcc accatccaat
     4081 ttattcgcaa cacagaatcc gtctgcttga agtatttgaa ttacgcgctg taaataaagc
     4141 tctctgttaa atcctcccgt ggaggaattg acggatttat aatttcgttg cacggcaaag
     4201 cccaagagcg cgagatattt ctcagaaggc cgcagcccta atgaacctcc tggtccggtt
     4261 gcaatttcaa ctccatcagc tacacatttt aattctgcac ttcttaaaaa aagttcatct
     4321 tttgcccgag agaaaagtgg agttaccaga agccctgtca gagccatcag tttgataaaa
     4381 cgaagcatga cctctcctta aataaagaaa aattattcta agaatattat gctttattta
     4441 aggcaaatcc cacaaggccg ggttgataaa ggccctggga gaaagtctag gaagacgctt
     4501 aaacgtttac ttcaaatgcg ctttgagctc gtccatattg aacagggctt gcagtttgag
     4561 tcctacttct ttaagctttt cttctccgcc gcctcgatgg atcacgcaaa gtacgtcggc
     4621 cacttgcgct cccatcgaac gcaggtccgc ggtgctaaga atcacttggc cgccagttgt
     4681 gacgacgtct tcaatcacac aaagtttcag gccttttatt tcgaggcctt cagcaaattt
     4741 gcaagtgccg tactctttgg cttctttgcg aacaaagacg cagggaatgc ccgtgatcaa
     4801 ggataaagca gtagcgatgg ggataccccc catttccagg gccgcaaaag cttgagttcc
     4861 cggaggaatc agggcgcgca tctgaagggc aatttcattt agtaaatccg gacgggcttc
     4921 aaaccggtat ttatcaaaat actcatggga aatttgtcct gagcgcaatt taaattcacc
     4981 cgtaagatgg gccacttcgt aaattttttt tgcaagttct gttttgttca ttttgaaaaa
     5041 gatttccttt gaacttctat ctattgaaac taacttcggc gcaattgttt tttgcggatc
     5101 tgccactggt caagagcata ttttctcatg tcttcgatat tatcgaattc atccacgatc
     5161 tcaacgccga ggagagtttc caccgcatct tctaaagtca ccaggccggc aatcacacca
     5221 tattcatcta aggccaaggc taagtgttct ttctctttga taaaaaaatc taacacttgt
     5281 gccaccgtaa ggcgttctga aacggacgcg atgggggtca ccaagtccga gatttttctc
     5341 gtatgttggt catccgaaga ggcctccagg attttataac ggtgagtgat gccgataata
     5401 ttatccaagc tgtcgcgata aacgggaatg cgcgagaagc ggagaggtac atatttatta
     5461 accacttcat ccaccgtgat gtcggcttcc agggcaaaaa acaccgagcg aggagtcata
     5521 atatccgaca catagatttt atccaacatc aggagatttt taatcatgtt ggattccttg
     5581 gttttcaagg ttccttcgtt ggctccaagt tcagcagtca taatcatttc ttcacgagtg
     5641 acttgttgag tatcgggctt ttccaccagt tttcccacga attgagaaaa gctcaccaaa
     5701 gggtaaagca caaagatcat tgtttgaatg acgtaggcgg caaccggagc aatcaccttc
     5761 caatgagctg caccgatact ttttggtaaa acttcagaaa taatcaaaat gccgaaggtc
     5821 aaaataaaag aagacaaggt aactaagtct tctccataga gctgctggac tttgtaggcg
     5881 attgcagaag atcctagagt gtgcgaaata gtattaagag tcagaatcgc agagagcggg
     5941 cgatctaaat tttccttcag atgctggagc agtaagccgc tgcgctttct attctggacc
     6001 gcgactgcca cgtaggctgg cgagacggaa agaatcaccg cctctaacaa agaacaaata
     6061 aacgaaattg aaatagtgag acaaaataaa aagattatcg aggccataga tgtatgccgt
     6121 ataatagcac aggctagtga aagcttgtct acttatcctt ggcccttgac gcggtactta
     6181 ggtcctgagg ttaactaaat agccaataat atgttcaaga gcagcttgaa cgccaagaag
     6241 tgttgcaaaa ttagagactc tatttacaaa aataattata aatgcttcct ttaaaggcat
     6301 tcaaaggagg ggactatgaa aaatatgttc attagttttt ttattttctt agggggctta
     6361 gtggtcaagg ccgaagttaa aaccgaagtg gtggaatata agcaggatgg aaccactctt
     6421 gagggattct tggcctatga ccctagtttt aagggtcctc actcggcgat tttgattgtt
     6481 catgaatgga ccggcctcgg tgattatgtg aaaggccgcg caaaggaact agcggcaaaa
     6541 ggctatgtgg cttttgcatt ggacatctat ggtaaaggcg ttcgtccggc actccccaaa
     6601 gaggcagcga aagaagcggg caagtataag gaaaatcgca agctcatgcg agaaagagcg
     6661 aaggcaggtt atgattttat caaagcgcag ccctttgttg actccagcaa gatcatcgcc
     6721 atgggctatt gctttggcgg cactgtggct ttagagatgg gacgttccgg attgccctta
     6781 gcgggagtgg tcagtttcca tggaggtttg gccaccccaa ctccgcagga tgcgaagaat
     6841 tttaaagcga agcttcttgt tttacatggg gcaattgatc cttacaccaa agatgaagtt
     6901 ccaggatttc agaaggaaat ggatgaagca agagttgatt atcagttgat tagctatggg
     6961 ggcacggttc atgcctttac caatcctcag gctggcaatg atatcaaaac aggcgcggca
     7021 tataatgcct tatcggatag acgctcaatg caggctttta tgggttttct aaatgaagta
     7081 gctcctattg gtaaagctgc tactcattga aactgagcac tcatgtttat ttgcaggtcc
     7141 taggctcaga aatttcctga ccaaagtccc agaatgagaa acttggcatt tcctatgcta
     7201 cattgggaag aggtgagtat tagctcaagg agaatcgttt atggaacgta aactgaactc
     7261 atttttggct ttagggctca ttttcggcac tttttcgtcg acgcaagcgg ccccgatcgg
     7321 cagcccccag gactatatat tacaaaccaa atatctttgc caggctaacg gcattgtggt
     7381 ccgtaataag caaaagctcg tcagcaacgg gcgaggtgtt gacgaggttc aattcgtggc
     7441 aaatgcgggt aaaaaccaag ctttgatgtt tggtggtaaa agcgaatata tgatcttgcc
     7501 gcaagtaacc aatggaacct ttacgaaatc tttgaatgta ttatctcgcg atcgctcagg
     7561 aacttacaag gtcgtgcaag aagtggatat gggagagact caaaagacca aaactaacat
     7621 tggcctttcg gttaaagacg gacaaggtga ggctcggccg gtttcgtgtc gtatagaaat
     7681 ggaatggctt aagcgagcaa aataaaaggc tagtcatcat aagctagcct ttttatttag
     7741 ttaaaaaact caaacttagt tctgagcttg cttggggacc ttgctttcat cgacggtgat
     7801 ttcaatttca acacgacggt ttcttgaaag tgcgtctttg cttttccctt ggtctacagg
     7861 gttttcagag cccataccca cgaagctgat agtgctatcc ggaatgccac ctgccaccaa
     7921 ggcatcgcga acggattttg cccttttttc agatagaggt ttgttcacct tatctgtgcc
     7981 tgtgctgtca gtatagcctt tgatcgttaa cacgttctca gggtattttt tcataatgcc
     8041 agcgagttct gtaatgttgg tttttgcggc agcttttaag tctgcttttc cagagtcaaa
     8101 aaggatgtcg ctcttaagct ttgtaacaag accctgttct gtgcgctttg tttctgcaat
     8161 tttctcgagt tccttttgtt gtttatccaa acgatggccg atggcaccac caagacccgc
     8221 tcccagggcc gctccgatca tagcgccttc gttgcgtttg ccggtttgat gaccaatcac
     8281 agcaccggca acagctccaa tggcggttcc gattcctgcg tctttagccg cattcttgtt
     8341 atcgccactt gcgcaggcag aaagcatagc tgctgcggtc ccaaaaataa tgatttgttt
     8401 tttcatggtt taggctcctt aagtatttag aattgtagtg gatttattat tgaagatttc
     8461 ttcttaaaag atctagtcag gattcgccgc gtcttaaggt gggattcaga ctttagaccc
     8521 ttctgcgatg ctcgggcatg gacaaaggta gtaaaagtat atcaaatagc tttttttcaa
     8581 gatgaaggtg aaattatgag agacatgttt ggtgatgatg attcacaaaa taaaaaagat
     8641 gactttggtg ccatgtttga acaatccatc cagggctata ccaagtccta tggcaaaggc
     8701 gacaaagtca cagcggaagt cgtaacttta gggcgtgatg aaatttttgt aaatgtgggg
     8761 gggcgcgatg gcctagttcc gcgttcagag tttgtggatg ctgaaggagg cctgaaagtt
     8821 cagatcggag atcaggttac cttgtacgtg attaaatctc aagagggact attggttctc
     8881 tccgcaaagg cttcgagcaa agccatgtct gattctctag aagatgcctt tgatttcgaa
     8941 actccggttg aaggacgagt caccgaagtg gttaatggtg gcttccgcgt aaacgttatg
     9001 gggaaatccg cattctgtcc tctgagccag atggactcca agcctatcac agaaccagag
     9061 agttatattg atcgcaaatt cgaatttcaa atcacgaaat ttgaacaaaa aggccgtaat
     9121 atcgtagtgt ctcgtcgtcg catcttagat atggaaaagc tggagaacca aggcagcttt
     9181 atggacaccc acaaagcggg tgatcttttg cagggccggg tttcgcgcat tgaaaactac
     9241 ggggctttcg tggagcttgt ttctggtatc gatggccttg tccatatttc tgaagtgagt
     9301 tggtcacgtt tggcgcatcc atcggaagcc ttggctgtgg gacaagaggt ttcagtaaag
     9361 atccttaaaa tcgaagagga tgcactcggt cgtttaaaga tttcattatc atgcaaacaa
     9421 gctgaagagg atccttggat gacggcatac aaagattttc cggtgggcag tgtccataca
     9481 gccaagattc ttagcaagga acgtttcggc tttttgatgg aaatcaagcc tggcatcgta
     9541 ggtcttttgc caaagagtgc ttttcgggaa gtggctgatg aacgcgaaat ggagaagaaa
     9601 aattcaggtc aaactttgaa ggtacaaatt caaaatgtaa atactgttga gaaaaagata
     9661 gccttatcat tcccgaaaga ccaggaagac acttcttggc aaggcttcac gggtagtggc
     9721 actgtcagtg gcaaagggct tggaaccttg gctgatcagt ttaaaaacct aagccttact
     9781 aagaagaagt aggttctatt tacaacaggc cctatagggc ctgtttgagg ctctcaaagt
     9841 tcatgtctga ccttctggag gagcggagac tccagctcac tggaaaagaa ttaagatgaa
     9901 ttatggaacc cctaatttag gtaagagctg cgattctatt taacttctgc aaaactaaat
     9961 caatcgattt ctgccagaac tgcggctttc taatatcttc atcgagatgt ttttggatga
    10021 gatcctccgc ggtcattcga ccggtatcgc gaagaatacc taaataggct ttcataaaat
    10081 ctgagcctag ttcttttctg cgggcgtaaa ggctaagact aaagagataa ccaaaagtgt
    10141 atggaaaatt ataaaaactt tgatctccag cgatggagaa gtgcaatttg tgagcccaaa
    10201 agagatcatc atttttactg agaaccggcc cataccattt tgtccaggcc ttgttcatca
    10261 gctcacttaa ctcttgtgga ttaagcgctc gttctgcgcg tttatcatag aagctttttt
    10321 caaaatcaaa gcgggcagga atatttatca aaaaggctga tgaggactcg aggttactcc
    10381 aagcacagtc taatttttct tcgtgggtgg aggccttttt taaaagaatc tctcgtaatg
    10441 cagtttccgc gaatacactc gctgtttccg ctagggtcat tggataattt atttgtgctc
    10501 taggcagatc tctcatcacc caagcgtgaa agccatgtcc taactcgtgg gcaagcgttg
    10561 aaacatcttg atcggatccc aggtaagtca cgtagacgcg gggttctttg gttttcggaa
    10621 aattcgtgca gtaagctccc gttgttttgt tcggaagaac gcggccctca atccactggt
    10681 ttttcaccat gagatctacg aaatcagcca tttgtggatc tacatcggca aaggcctcgc
    10741 gaataagaca aatagcttcg ttaaaggagg tttgctttcc ggtgtcgcct aaaggactgg
    10801 gcgctagaag atcccaagag tccaattgag acttcttaag aagttttgcc atcatcgctg
    10861 gagctttgcg agtctcttcg agatttttac agcaggcttc cattaaagct tccagcgtcg
    10921 cttgttcgat acggtttaca tgaagagggg tatctaaata atgtagaggt tttttgtgcg
    10981 aacgtttttt atttacttca tggcgccatc ctaccttcca cttcggactc tttcgctttt
    11041 gcgattggat cagtggcatc caccgagagt atacagtgca cgtaagtgaa aagattgccg
    11101 agggtagttt cggctttttc acaaaggata aaaagatctt gaagcgtcgg aatatctttg
    11161 cttgagttct tcttttcaat ggtgatgata gcattggaga caaaggcaaa gtcctcttga
    11221 aaagcaggac tgtcgacgga ggggtgttgg gactcgagat tccaagcggg gggtgtcata
    11281 ggctctccta gatatcaaaa aaaagccgat gtttccatcg gcttttttta ttttcttgtg
    11341 tgcaaaacta tttttgttcc gcagcacgtt tagcttggta ttttttaacc aattcttctt
    11401 ggatatttcg cggagccgct tggtatttcg caaattccat agagaactcg cctttacctt
    11461 tagtgcccga acgaagatct gttgagtaac caaacatttc tgtcagtgga acttcggctt
    11521 cgatcacgca gtttccttcg aacgaagtcg taccaacgat aacgccacgg cgttggttga
    11581 tttgacccac tgcagatcct tgatactcat ctggaactgt tgtctcaagc ttcatgatcg
    11641 gctcaagcac agttggtttt gcagatggat aagactcacg aagagccgcc attgccgcaa
    11701 ttttgaaggc catgtaagaa gaatccacgt catggtatgc gccatcagtg agttcaactt
    11761 caacgccaac gattgggaaa ccaatgagag gtcctttaac cgtttgctct ttgaagccct
    11821 cttcaaccgc agggatgaat tcacgaggaa tacgaccgcc gacaacactg tttgtaaatt
    11881 taaagacaga accatcttct tgcggaggca atggacggat tgaacccacg attttcgcgt
    11941 attgacccga accgccggtt tgctttttat gagtgtaatc ataagcggct tcaacagcga
    12001 tagtttcacg gtaagcaacc tgaggttggc ccacgatcac ttcacagttg aattcacgct
    12061 tcatacgctc aacgtagatt tccaaatgga gttcgcccat cccggaaatg atcgtctcat
    12121 tggactcatc atcacggtga acacggaagg taggatcttc tttacggaac ttttgaagtg
    12181 ccttagtaaa gttgttcgca ccttctttat gcttaggagc aacggccaag ctgataaccg
    12241 cgtctggaac gtgcatggac tgcatagaag cttcaatttt atcgtcacag aaagtatcac
    12301 cagaggcaca gtcgataccg aacaaggcga cgatgtcacc agcgtaagaa gtgtcaatgt
    12361 cttccatttt atcagagtgc atacgaacca aacgaggaat ctttacagat ttcttattgg
    12421 tttggttgac gatgaagtca cctttgctca ttttaccttg gtaaacgcgc atgtaagtca
    12481 actgaccaaa ttgagtctct tgaagtttaa atgccaaagc cacaagtggt ttgctgttat
    12541 cagagaacaa ttcgaatttt tcttcatttt tagttaaatc aagagcgtgt tcttttttct
    12601 cagctggaga tggtaagtag taagtcactg catccatcaa acgctgaacg cctttgtttt
    12661 tgaaggccga accgcagaga acaggaacca atttcaagct gatagttccg gcgcggatag
    12721 cggcacggat ttcttcagtt gtgggctctt cttccatcaa gaatttttct tcaatttttg
    12781 gatcgatgtc agcaagtttt ccaatcatga tttgacggta ttcttttgct tgctcaacca
    12841 tgtccgccgg aatgtcagtt acattcacgg tttcaccgtt gtcaccttca ttgatgtaag
    12901 ctttcatatc agtcaagtcg acgtggccac gatgttgatc ttccaagcca attggaattt
    12961 ggatcataac agcgttcaat tttaacttgt cgatcaaagc atcggtcacg cggaatgggt
    13021 tcgcgccctg acgatctaat ttatttacga aagccaagcg tggaacgctg tagcgtttca
    13081 tttgacggtc caccgtgatt gattgggact ggactcccgc aacaccacat aacaataaaa
    13141 tcgcaccatc aagaacgcga agagaacgct ctacttccac cgtgaaatcc acgtgtcccg
    13201 gtgtgtcgat tagattaatg gtataatctt tccaatgaac ctgagtcgct gcggactgga
    13261 tggtaatgcc tttttcacgc tcaagatcca tggagtccat ggtcgcaccc acaccgtctt
    13321 tacctttaac ttcatggatg gcatggattc taccgccata aaacaaaata cgttcagatg
    13381 tagtggtttt tcccgagtca atatgagccg agataccaat atttctgacc atatctatat
    13441 tccatttttt ggacataact gtaccctttg tatgttaatg attgcttaaa gagtctatcg
    13501 agttaacatt atttgggcat catggcaaaa gaagaatgtg acgatttttt gacgaaagtt
    13561 cttttgtgca gtgtttaatt atgtaaggat ttatcgagtg aatttcctcc agattttaac
    13621 tgaaaacttt ccctataaac attttcgtgg cgaacaggaa aatattctca aacgggtgat
    13681 ggccaggcag gatactttgg ctctcatgcc cacgggaatg ggaaaaagtc tttgctttca
    13741 gtttccagca aaggtgcaaa aggacttggt cctggtgatt tctccgttga tagcattgat
    13801 ggaagatcaa gtacaaaagg ctcaaaaact tggcatttca gccacttatc tcagctccac
    13861 agtcagtcgt gcagaccgcg aaagtcgcca ggaacgttta gcgagcggcg attaccaact
    13921 cttctatgta acccctgaac gttttcggaa agaagagttt cttagcgctg tcaaaacacg
    13981 ccaaattttt tgcctagcca tcgatgaggc tcattgcatt agccaatggg gtcatgattt
    14041 tcgccccgat tattctcgac ttggcgaaat tcgcaagctt ttaggcaagc caccgatttt
    14101 agctctcacc gccacggcaa cgccagcggt gcaaaaagac atcctgaaga atctaaatat
    14161 ggaggaaaat ggagagatca tctttggcgg attggagcgc cccaatcttg agttgaatgt
    14221 ccacgacgtc tatgggatcg acgagaaaat tcgcgccatt attggtcttc gtcatcaagc
    14281 tctgggggcg ggaattattt attgctcttt gattcagacg ctcaagaaaa tttctggcga
    14341 tttgcgtcgt ttgggagtgg aacatttagt ttatcacgga gaccttcctc ctccagatcg
    14401 aaaacgtaac ttgagaagtt ttacttctca ggaaaatcct ttgatggtgg caacgccggc
    14461 ctttggtttg gggatcgata aggaaaatgt gcgcttactg attcatgcgg agattcctgg
    14521 ctccatagag gcttattacc aagaggtcgg gcgtgcgggc cgagatggga agccctcaca
    14581 gtgccatttg ctctatgatc aagaagacgt ttccattcaa atggagtttt taaagtggtc
    14641 gcatccagag cctgaattta tcgcgcaagt ttataagttg attgataaaa atcgtggtcc
    14701 tttagatcaa gaccgtttcg actatttgcg tgagcaaatg agttttaaaa atcgtcgtga
    14761 ttttcgcgtg gagagcgcgg tgaacattct tgagcgttgg ggctgcttgg ccagtgctga
    14821 aactcccttt cccttcgtga ccgttcagga gcccacaaag gaaatgtttg agaccgagaa
    14881 ttctccttac cttttaaaaa accaaaacca aaagctttat gatatggttc gttatgctac
    14941 cagccaagag gagtgccgtt tgctgcaggt ttacgagtat tttggttatg agggaggcca
    15001 aaaatgtgga cactgcgata tctgcggtgt cgaaatacag gctaaatcat gattattgtg
    15061 acgatttcat cggtttttag ccctcttttt ttctaaagta cactttttcg tttttattgc
    15121 ttcccattct gagaagaatc gactaatcgt aggagctcaa ctttcagtcc ttgtagagcg
    15181 tatcgggcat agggcccatg ttcctcttaa gtggctccca attgccaaat tttaggaggc
    15241 tcaaatgggt aaaaaaatat atgtaggaaa tctttcttac tctcttaacg atcaatcatt
    15301 ggttgatgtt ttttctgaat tcggaaacgt tgaatcagca cgtatcgtaa ctgatcgtga
    15361 atctggtcgc agtaaaggtt tcgcattcgt agagatgtct agcgatgacg aagctcaaac
    15421 tgcaattgca aaattgaacg gcgttgaact tgaaggtcgt gcattgaatg tgagcgaagc
    15481 taaaccaatg gcgcctcgtg aaggcggcgg cggacgcggc ggcttcggtg gcggcgggcg
    15541 cagcggtggc ggcggacgtg gcggcttcgg cggcggtgga cgcggcggtg gtggcggcgg
    15601 acgctactag tatttaaatt atagaatata gatttctatt aaaaaaaggg ctgagtaaat
    15661 cagccctttt ttattttaaa cccaacctaa gttcattttt aaaaaaccta ggattcctgc
    15721 cgctaggatt agccacgcgg agtttacttt atagccaata agcaaccaga gacttagtag
    15781 aaagatgata atttcgtata ttgactgtaa agactcagga gccagcaaga tcccagctcc
    15841 catcattaaa ctcaaagaag cgacattcaa cccatcgaga agtggggaag tccattttga
    15901 tttacgaagt ctcgaaatta aaggtgctgt tattgccaca aaaaagaagg ccggagcaaa
    15961 tattccacaa gtcgcggcaa tggctcctcc atggcccgaa atcaagtagc caataaaagt
    16021 ggcggtggta aaaacaggtc cgggagtaaa ttgcccgacg gcaatggcat cgagtaactg
    16081 ttgttgattg agccattgat attggtctac gagatcgttc tttaaaaagg caataagaac
    16141 atatccactg ccaaaaagaa cgctccccac ttttgtgaaa tagacaaaga cattttcgac
    16201 gggaatgcga tggttttcat tcaacgaagc ccaagaagga aggccaatta gaaacatcgg
    16261 cattgctaaa aaccacaaag atatcttccc tgctcctggg ggcttttgtc gcagaaaaag
    16321 attggtgatg gcgatggctg caaggatgat caattcattt attcccctcc cataaagaaa
    16381 aatagaaatg gcacaaagac cgaataaaat tttatcttta atggcattct tggaaaggct
    16441 ccaaatggcc tgtgcaataa cagccaggat cacaggtttg atagatagaa aaaggaattg
    16501 aaactgaggc agtgagctaa atttaacata actccaagct agaagccaaa cgatgaggaa
    16561 cgctggaaaa ataaaacaga ttccaccaag aaagagtcct cgccagcctc ccattttgta
    16621 gccgatatga atggccattt ctgtggaatt ggggcccggg attaaatttg tagcgccaat
    16681 gagatctaag aattcttcat gggaaagcca cttctttctc cgcacaagat tttcttccat
    16741 catagcgata tgtgcggcgg gtcctccgaa ggcagtgctc ccgagtttta aaaaagtagt
    16801 ggcgatttca tacaagggcg gtggctttgt ttctgtcatg aagaggcctt attctaagat
    16861 cgaaaaattg tgaagtgttc aaaggctttg ggttcattga tagcgtagag ccatgggtga
    16921 tcttggtaaa tccaatgccg aatgccgcgg cctcgagcga cgacggtaat ggacctttcc
    16981 agggcaataa ttttgagatc ttcaagagtt tttgcaatgg cagaccggct gccgaaatca
    17041 aaaataaaat agtaatccgc aagccttgga gtaaaatccg gagaggagag atcttgagtc
    17101 tctagattga caaaaggata gttgaacttc tgaaggatgc gatttccttc ttgaactcgt
    17161 tcgaaaataa gttcatatcc ggtaaaatga acttgcggaa agtgtcggcc gaggacgtgc
    17221 gccatacgac catagccgca gccaagatca atgacggatt catggggttg tggattgaga
    17281 atatttaaga tggtgtgaat ttcagtgtat ggagtttgca tggcttgaac tggcaggcct
    17341 atccaagctt gaaccggagg gtgttccttt tgcagagtgt ggtttttaaa aagcgttttt
    17401 tcaatttcct cggtgttaaa acccagccat atgtctacta aaatcgagtg ttcttgagcc
    17461 tcgtgatagg aaatcgaact tcccattaaa agagagaaag gattttctgg attaaacttt
    17521 tgcttcaaat taattttaga caaggtgtag atcctttatt tgtcccagaa tgatttaaaa
    17581 agagacaagt tatggaagga ttaataaaat ttaattccga tgtcatcggg ttttaatatt
    17641 ctaggcttaa gatattttaa gtgattctta atttatttaa tataggagat tacatgaaaa
    17701 gcctcgtcgc aattttcgtt ttactaggtt caagcggtgc atttgctcaa gggaaagagt
    17761 cgagaccttg ccagcaaatt aaatcagcat gtgaagctgc gggatttata aagggcggac
    17821 ataaagaaaa aaaaggtctc tataaagatt gtttggagcc aattatggca ggccaaagtg
    17881 tagctggagt gatgattggc gccgatttga taactgcttg taaggataac agacaagctc
    17941 ataataaagt aaagtagttt ctacgttcgt ttttcaattt aaaaggtcgg aaattcagat
    18001 gcgtaaaaca gctagttacc aaaatgaatc gtagtacttg ccccagcggt tgcttcggga
    18061 gctgtactgc ttttttcgct agcgaggtgt tttttgttaa attgatcttt tacagtgttc
    18121 aggcaagtat cataaccttt ttcgccaacg cgataggttt ggccagtgga ggttgtgcaa
    18181 gtggtttgga tagccatttt tgttccaaca ctttgagtgc cgctcataag gaattttcga
    18241 tactcttgga aattaagatc ctgcgctaat gccaaagagt tgaacaaact gctgaatagc
    18301 aaaattattc ccataaatat tgatttcata gagtctcccg cagtttttca acctacaaag
    18361 gccaaagagc ggaatcaaga gggaaaagtt tacatttaat gctatgtgtt gcaagagtct
    18421 atacggccaa cgtctaggac agcttatatt tagtgagaaa gttatttagc atataaggag
    18481 atgaaaaaat atctatttac tggtttggtt ttaacaaatg tatttttagc ctgtggacga
    18541 gtccctaagc tcggacctga aactgatacc aagcttgcta cagcctctga agaggattgt
    18601 ggatttgtgc agaacatata cgggcagcga gtttcctgga aacaaagctt tcccgtgaaa
    18661 atttatatcg atcccagctt tcccgttgag tacaacccag ttttgacagc tgccgctcag
    18721 aaatgggaaa gtgttctagg taaaacttta tttgtttttg aaagattgaa gcaaacctcg
    18781 acgccgggaa aggataatca aaatgttctt tactggctca cgtcttgggc cgagagcgat
    18841 aaaactctcg aggcagtgag ttctctttca tggacaaata atcagttggt ggaaacagat
    18901 ataaaaatcg atgctcagta ttattcttat tatgtaagta ctccagtttc aaatacagag
    18961 gttcatttgg agagcctttt agttcatgag ctgggccaca ttttgggatt aaaacatgta
    19021 agtaacagca taccttccgt gatgttacag gttcttgatt atttgttgaa gcgcgatacg
    19081 ccgacagcag aggatcaggc gaatcttaag tgtgaatata attaggagat atgtgaaaac
    19141 ttcaaaaagt cgttctttag tgggccttgt acttttgttg attgggggat tgtatcttgg
    19201 ggatcgcctc tttttgcaaa aacgtggcat tgcatcattg agtggaggca tcatgtcttc
    19261 gaatgatgtg aattattttg atctcagcga actctccggt aaagattttc aaagagcctt
    19321 taaagtggct ttagctcaag gtctgcaagt ccaaaagagt ttacaaggcg taggcttgtc
    19381 tttaggtctt tttttagtac aaagtggaaa tggatccaaa gtttatgcct gtgaaaaata
    19441 tcccaatatc gaagtcacac tcaaagcgga aggggtcgct cattctggaa atattcccac
    19501 ccttattgtt cgcggtcctt gtttggtttc ggaagatgga cacaaaattc aagcttttgc
    19561 ccttcctctc aaggatctct ataaaaactt aagagaaaat cctatgtggc gggtgcctct
    19621 cggaaatcga ggagattctc ttttactttc tgctcaatat ctctacaatg aatggccgac
    19681 aaattggaat gtggtacagg tcaaacttta tagtgatcaa gaaagtcttg agattgacgg
    19741 ttatgagatg atttcccttc ttgaccaacc cctgactttg gatttttctg aaggggagtg
    19801 atggatgcaa acaaaaagaa ttttcattct tccatagatt ttccagatcc tcgcgaagct
    19861 atggccgaag gggttatcgc tatcggcggg atattggacg tgggcacact ttataccgcc
    19921 tatagtacgg gtattttccc ctggcctcat ccggattatc cgctgttg
//
LOCUS       DATBTE010000399        22159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2309536, whole genome shotgun sequence.
ACCESSION   DATBTE010000399 DATBTE010000000
VERSION     DATBTE010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 22159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22159
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2309536"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VIG33_15340"
     CDS             complement(<1..378)
                     /locus_tag="VIG33_15340"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEY8272264.1"
                     /translation="MTVAAVMTYLAQAYIKKIEGAKKSKDSEVKVQASEQAFEKFSKQ
                     NGESLTPSFSETPSRPYKTVRQKRKALSLTIRRAVFEKAQHCCEYTHPLTGKRCGSEF
                     QLQIDHVRPLACGGSDEIKNLRAL"
     gene            complement(332..868)
                     /locus_tag="VIG33_15345"
     CDS             complement(332..868)
                     /locus_tag="VIG33_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272265.1"
                     /translation="MPIPSPEYALDLSKVSNQEILFRLERLSRTERKITHLVLSHINE
                     VEVRKLYLDLGFTSLYRYLTQHLYYGEKAAYDRMQAALVLKHTPEIARKIEEGALNLS
                     QLVQIDQSLKQERKAGKELSVKATQELLGKLENKSVFETGKILASELNQTPKTQQKVK
                     PQKDDSGRRDDLFSSSLY"
     gene            1440..2099
                     /locus_tag="VIG33_15350"
     CDS             1440..2099
                     /locus_tag="VIG33_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272266.1"
                     /translation="METPVKYLIVCLLVVASLVACSKKSDNREARAPEVGLGVPGNTP
                     TCTAGQQAVGRIYESGNTASNSSLTFEQRVKGLLSATVDPQFFGTISGSGNDAQNGVT
                     IEGRLRYDANGTVLLDQSNVKIMIYDSFVGQLDSQQQVIQAYPINFNAAAAGTMNLQT
                     KVFMLKFKDEFGEITLNGTVNAANVSGTISYQNDKNVTGGSLSAGFLGEFSIATCGWI
                     N"
     gene            2157..3251
                     /locus_tag="VIG33_15355"
     CDS             2157..3251
                     /locus_tag="VIG33_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04552 family protein"
                     /protein_id="HEY8272267.1"
                     /translation="MPQKFSFDLDMLNSVAGGRSAIDTPRLNIKSLEQATSFIQSYGF
                     DYSNVQDQEKIWYYHRRALVLIVEKLGFDLQEIPELLRERKALVDIRRLLLLASSGEA
                     SERSLQRWSCAILRCMHVYVHAENDLFSSFSEEIQSQILTPFQKAIYHDGTTHKAYLK
                     SLSREGREPVELLGFEVKPFKTSASTVIKLLAKPDALALKIFDKLGVRFITRSLFDSF
                     QVIRFLVEENIMSFPHIMPDQSSNNLYPVELFVSVCKELLESGKKYSETELKGIFDEK
                     LRSLGEGAQFFRKENFFSGEDYRFIKFITRKLIHIQPQAGKEPFSFFYPFEIQIMDQS
                     AHQSILSGPSEHMAYKERQRLAARKRLFSE"
     gene            3263..3994
                     /locus_tag="VIG33_15360"
     CDS             3263..3994
                     /locus_tag="VIG33_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8272268.1"
                     /translation="MRLVASLRSILMTLIYPLVLVFSSILVMAVNLFFNNKKWDDYIV
                     QLWARFSCWMFGVKVKVRGAEHIPAGGCVFLFNHTSFFDIFTMQGALNGFRFGAKIEL
                     FKIPVFGLAMRRLGALPIARHNRDEVFKVYEAAQERIHRGERFALAPEGTRQEEEKLG
                     PFKAGPFVFAINAKAPLVPVVIRGAAQILPKGCWIPNLDAWTREVTIDVLKPVSTEGY
                     DIKDRPVLQEKIHKQMEAYFTNVHS"
     gene            complement(3970..5316)
                     /locus_tag="VIG33_15365"
     CDS             complement(3970..5316)
                     /locus_tag="VIG33_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272269.1"
                     /translation="MRISFLVFLSLNTSVWSFAAEIPALSFYRNQQSLFPSGQASRED
                     LEEKVLRRDYELWLRVSWNKKTYEIPGETVIRDVQVTKNLISKESLDLLKDPQETSSR
                     ITTVSANTTLQVLHTNSYWAELLETKTKLRGWAPLHLLEAPAEDQGVFITLIDTFLRK
                     TPQDSSGIITTVPRLQRLTPLGIEKSYLKVSYQGHIGYLDLANLTGRADFAMWAYHQN
                     KGWLGISHRENEYLFTVDNQKIPLQGFLAFNPYVSRGVVCQKLKEEAPSIRSRVEIAN
                     NKAHRWTLSLLEGHGSVWWRMDEPTISYKPLAENQITSEQLLKREVSAVAFAAKSIKG
                     LVSANGIFRTEDGKIWKEIALFEGKNFPVAIHPDGIWYVGNYRSFDEGKSFETYIKWD
                     KLAEQIQFSLHKTPRHLRITQIDPISHSRLQILIDTGTQKIKMRAHILSHEWTLVK"
     gene            5493..6380
                     /locus_tag="VIG33_15370"
     CDS             5493..6380
                     /locus_tag="VIG33_15370"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEY8272270.1"
                     /translation="MGIEGNKHIQDVRAIGVVGAGQMGNGIAQVAAQFGYHVVMVDVA
                     MAALEKGLATISGSCDRLIKKASMTEAQKHELLERIKTSQDMAALKECDVIVEAATEN
                     VDLKLKIFKQLDEVAKAKAILASNTSSISITKIAAVTKRPAQVVGMHFMNPVPLMKLV
                     EGIRGLQTSDETFKTVKALSEKMEKVFVESVKDMPGFIVNRILMPMINEAIYTLHEGI
                     ANVESIDNAMKLGTNQPMGPLTLADFIGLDTCLAIMNVLHEGLGDSKYRPCPLLVKYV
                     EAGWLGRKTGHGFYDYSQK"
     gene            6394..7194
                     /locus_tag="VIG33_15375"
     CDS             6394..7194
                     /locus_tag="VIG33_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY8272271.1"
                     /translation="MSQFSYKAINLELKDNGVWVLTIQRPEALNALNAQVLNDMADAL
                     RQIGEMNFENTRALIITGSGEKAFVAGADIKEIAELDEEQAMAFAQRGQSIFHELNLL
                     KIPVIAAVNGFALGGGLELALGCDFIYAAEGAKFGLPEVSLGLIPGFGGTVRLARAVG
                     IRKAREMTYTGDMISAAEALSLGLVTKVVPQAELMSTVMKAVNTILSRGPIAVAAAKK
                     SINQAYELDIDEAQKTEAQHFAYLFTSEDVREGTRAFIEKRKPAFKGC"
     gene            7200..10499
                     /gene="icmF"
                     /locus_tag="VIG33_15380"
     CDS             7200..10499
                     /gene="icmF"
                     /locus_tag="VIG33_15380"
                     /EC_number="3.6.5.-"
                     /EC_number="5.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0047727 - isobutyryl-CoA mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fused isobutyryl-CoA mutase/GTPase IcmF"
                     /protein_id="HEY8272272.1"
                     /translation="MATAPNYTPKNPIRIVTAAALFDGHDAAINVMRRILQEQGAEVI
                     HLGHNRSVNDVVKAVLQEGAQGVCISSYQGGHMEYFKYMKDLLDMNGASYVKIYGGGG
                     GVIVHDEKHELEAYGIDQIFHPDDSRRLGLEGMIQVIVRACDFDVTAAKVKVIEHKVE
                     NPFKADSIPSFDLGRALTFIENGVVEEEVNKLLQGSALDRFQTKFKSKFPSKKETPPV
                     LGITGTGGAGKSSLIDEMAQRFLNAYPEKKIAIVCVDPSKRKTGGSLLGDRIRMNSLS
                     RHHIFMRSVASRGSGREIASSLPGILQYLKKFDFDLIIAETSGIGQGNTAITEVSDLS
                     MYVMTSDFGAQSQLEKIDMIDFADLIAVNKADRRGASDALRDVTKQYKRSRKIFDEKA
                     KVPVFLTQASQFNDGGVNKLFFKLSELLDEKYTQNNWKMEESYKANILSAEEHGIIPP
                     ERQNYLAEIVSTIHKYKERTAKLAEKASLLGVLHNASALLNSPAENVKKAEKSLEEDF
                     TNEELHSLREWNEFQQRYACEELVFKVRGKEIRQPLTTESLSGTKIRRVVLPKFKDWG
                     DRFSYLKLENVPGEFPFTAGVFPLKRADEDPKRMFAGEGTPERTNRRFHYLTKGESAP
                     RLSTAFDSVTLYGQDPDPRPDIFGKVGESGVNICTLDDMKKLYAGYDLCSANTSVSMT
                     INGPAPMILAFYFNTAIDQQVEKKEKTLGRQLSPSEWQEVADWTVQQVRGTVQADILK
                     EDQGQNTCIFSINFALKMMGDIQEFFVRKNVKNFYSVSISGYHIAEAGANPISQLAFT
                     LSNGFTFVEYYLSRGLNIDDFAPNLSFFFSNGLDPEYAVLGRVARRIWAIAMRDLYKG
                     NDRSQKLKYHIQTSGRSLHAQEIDFNDIRTTLQALLALYDNCNSLHTNAYDEAITTPT
                     EESVRRAMAIQLIINREFGVSKNENPNQGSFFLEELTDLLEEAVLSEFKRINERGGVL
                     GAMETQYQRSRIQEESLYYERLKHDGTFPIIGVNTFIDPRTLQSDYVRPKIELARASY
                     EEKNQQLENVRNFQKEERTQAAQELKNLKAAVLKGENIFVALMKAARHCSLYQMTEAL
                     YEVGGQYRRNL"
     gene            complement(10525..11730)
                     /locus_tag="VIG33_15385"
     CDS             complement(10525..11730)
                     /locus_tag="VIG33_15385"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8272273.1"
                     /translation="MAHLRVRYIQNMIEKAVHLSPLVGILGHRQVGKTTVLEKMCDTY
                     RTLDKKTELNKAQFETENFLKYYPKSLQGIDECQLAPALFPALKEFVRIHKSPGQFLL
                     SGSVRFTSRQAIRESLTGRIVNFELLPFSISELAHRELPQTITTILNLSTMDRVAQSL
                     SNSKISGLEKQYNYYFEHGGLPGVCFIQNKNLRHLRIKEQLQTLLDRDVRLVYPTNLS
                     FSQIQDFFRYIAQNQGQDFNYSAAQKAIGLSPVTQKKLLQAFEGIFLIRRLRIEGTKK
                     GDIFYLEDQAESFFMLDGQQTEKDQLEQLAYRNLRTQWYYQLGSTTREFHFLTRGGAR
                     LPFCIEQGGKRLGMLPIEEELPNRSEKSSAASFLKKYENARVLFLHNKKRLHVIDERS
                     LSAPVLFFA"
     gene            11992..12579
                     /locus_tag="VIG33_15390"
     CDS             11992..12579
                     /locus_tag="VIG33_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272274.1"
                     /translation="MKFIFCLFTFILFLSEFDSAFATESEYIEIHEAFYPRIELTLAG
                     GLALQTGYLTTKASLGTYIFGVNFVMPGGLMSFDWQGFHADHDTGEFPLNNNKSVTVS
                     TFSFIPHVRVFNHEAWNAYVGLGFTQVSLYQTSPEYMVDYGSFVFSGLLRYELNNKWS
                     VLYKTQWYNVNKTENDQKTSFEVWNHAIGAGYSFF"
     gene            12701..12970
                     /locus_tag="VIG33_15395"
     CDS             12701..12970
                     /locus_tag="VIG33_15395"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY8272275.1"
                     /translation="MNDKIKVEKAFLFGSGARGELTPDSDLDILVVLKNIEDMKAAYK
                     VVGQNLFSPIAVDWIFKDAKSFEERKDFGGVCFEAFHHGVQIYGQ"
     gene            complement(13034..14845)
                     /locus_tag="VIG33_15400"
     CDS             complement(13034..14845)
                     /locus_tag="VIG33_15400"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEY8272276.1"
                     /translation="MVHNWILIFLFAPCWVWGFSSERTQEYKKIILAQKLFENPQWLK
                     LGHYHKSWYGTYHSQLKGNFFIAQDGADNPQAELLATIDTLFSAPSSPSPQCRYLART
                     RWLKSVLPLAAEDVRGCPDRDQWKKQLGAEEAYIVFASSDLNSPGSSFGHTFLRLHNP
                     DHLHELELLDYGINYAALTGHDSGALFALKGLLGFYPGNYSMLPYHQKIREYINLEGR
                     DLWEYKLSLSTDDIEFLIDHLLELDGSFSYYYFADENCSYQILELLNVLRPQLDLAQS
                     FHDFVIPLDTLKVLARQDLLADEKSRSSLQAEWRSRYAGLNLQQKEELTNITKSPQLF
                     HFTAELTNKEKAETLEASLSYLSIKEFREQKELKDDKYALAVQRSKLGLITEPVSIPK
                     PSSPLLSQNPKALYFGYGQENHHNYLDFKFRRAFHDLLSHDTGLAPFFHLETLSFEFR
                     YFSQQKNLDLAHWTFLNVLATSPTNILDHPLSWTLDLGTRPKLVPYLDFGGGTSFDLS
                     LKIPARWVLLGRSENRTENENYAGYLGVENILITQWTQNFRSLLDVRYLYAYQSGGFL
                     WHQQWGVSYSSKAQEFRLEYRNRQNTPDWLFSYIFFF"
     gene            complement(14835..15323)
                     /locus_tag="VIG33_15405"
     CDS             complement(14835..15323)
                     /locus_tag="VIG33_15405"
                     /inference="COORDINATES: protein motif:HMM:NF022666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HEY8272277.1"
                     /translation="MKKLLGIALLSSACFLSPSAFAGDAGCGLGGIIFTKNAKLLQLF
                     AMTTNSFLFTQPLGITFGTSGCSSSGIVMNDKQIQYFVEINQEELSREMAQGHGEKLS
                     TLALLKGCTSQDSQKTFAQFTQSSYSHILPSANTSPVDMVQNLNTEMAGHSEVSQACR
                     GT"
     gene            complement(15390..16925)
                     /locus_tag="VIG33_15410"
     CDS             complement(15390..16925)
                     /locus_tag="VIG33_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8272278.1"
                     /translation="MSRIFQNLEVPIDADLEEKLQYLMPDHGPYRILRQSVDARRSRN
                     VHFVYTVEVAETNETLKIDNFPVEKIHKRVTEKPLIVGSGPAGLFAALRLVERGIPCI
                     LFERGSESSERIKGINRYWRYGELDRRNNVCYGEGGAGLYSDGKLITRIKSPHIPYVL
                     NRLVQFGAPVEIQWLANPHVGSDKIRRVIPKMREFLRANGCEIHFNTQVTEILFENKQ
                     TVGVKTEHGTEIRSLFVVLATGHSAEDMLLHLAEKEVFIEGKSFAMGLRIEHPQKAIN
                     KIQYRDFSEHPKLGSANYKLAHHDHKTNIGVYSFCMCPGGYVLSSGTEAEGIVCNGMS
                     NYNRNSPFANAAIVVSVDHEKNFGKDIFGGLKLRRQLETQAYQSVLAVGGSKELPAQN
                     MMDFICGRIGQVHPGSCPSGTKAVRLDELLPSFIHKRILEGFNKFERSMKGFITEESQ
                     LYGVESRTSCPLRVTRNEESLQSLSHQGLFPAGEGAGYAGGITSAACDGVRIAEKIFD
                     LCR"
     gene            16955..17782
                     /gene="asd"
                     /locus_tag="VIG33_15415"
     CDS             16955..17782
                     /gene="asd"
                     /locus_tag="VIG33_15415"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164316.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HEY8272279.1"
                     /translation="MSAITKFLPRNHLSYFIGKLVHWKGPRIWAEFTIRLFAKVYGIN
                     LKEAEKPVSGYPSLGEFFIRRLKPGARPLAEAAAVHPVDGHISQSGRIHNGRLIQAKG
                     KEYFLQDLLAHSEATKTYQNGFFITYYLCPTDYHRVHAPVSGWIRKVTHVPGELWPVN
                     KWSVENVHDLMSVNERIIVEMATDFGPTSVVLVGATNVGQIELSFDSGFRGNQLTLSK
                     NRVVEYKTPIPIEKGQELGMFRMGSTVIMVYAEEFANRFAGKMNLDLYVKVNQALTK"
     gene            complement(17800..18162)
                     /locus_tag="VIG33_15420"
     CDS             complement(17800..18162)
                     /locus_tag="VIG33_15420"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEY8272280.1"
                     /translation="MKFELKQHFQIESARFLPKLEKNHPCSRMHGHSFKIVLTLIDDL
                     DPHLGWVIDYNEIQAKMKPILSLLDHHVLNEVPGLENPTSELLAKWVYDKARQVLPML
                     VRVSLSETPTNECSYPVA"
     gene            18181..18681
                     /locus_tag="VIG33_15425"
     CDS             18181..18681
                     /locus_tag="VIG33_15425"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEY8272281.1"
                     /translation="MSTTSLQLAKQNFKFSSAHFLIFDENSAERLHGHNYQVRVEIGI
                     PDPAAIKGTGFFVDFNIFKKYIKSALDRWDERVLLPKDHPEMKIQVKGAVLEVNFRDR
                     FYAFPKNEVVLLPITNTSVEQLSRLLAEDFLKEFSSHSVSFISVYVEETPGQGATTNV
                     KSSLRF"
     gene            18788..19429
                     /locus_tag="VIG33_15430"
     CDS             18788..19429
                     /locus_tag="VIG33_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272282.1"
                     /translation="MRLILFLGLTAPFFSFEFAYAHTKPVDEQIQEVKEVIQPRAPFP
                     KTPAESTEGAVGCLECLGITVGTSRPPAKCDLKGARDDLKLLPKDWGLKATGADLLRD
                     EPLMKNLKYDHALGIVDTGLKLRGNPEAGQKLTSTSPEEDAGIFHGSHVTGIAVSDAH
                     GVNPYLATKVFPVGTNMGGKTTKSEDLLNSLEKAVDDKSTLAPPPLSQILVPM"
     gene            19426..>22159
                     /locus_tag="VIG33_15435"
     CDS             19426..>22159
                     /locus_tag="VIG33_15435"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8272283.1"
                     /translation="MRVYAPGSRIKSLNGAYNPNYFSTKSSVMMDGTSMAAPHFAAVL
                     TTLSNLAPELDAAAIGQLIKSTAINRGTGQKPFLFLNAYLAFQTLANARDCLKTSKSA
                     GQCLTESQQAVQQAAVLPQVPKGTACNDWQLYYTALRKVYFLTQGNRGVVKAIAELFA
                     GEDKPIAEKFLFLNRDSHEVQSSLLKDHTIPELYKEEQTRAMDFLTGKLDLEKVLAGY
                     DPSKYGRDDFENYTDGKEAPKNKHPLELLLPIKENFGDFLKYCEADLRSEKSQGPCRD
                     YVGNAPRQFKETLIDRLLQRDPPSTNSSILNGLNFGYDKAYDEKVQGLYQKAVKICLQ
                     GSLCKQNDLASFFSSSFVTREQKERLSQTILARAKKGDFGDDLSSGQKDAFITYWRTM
                     TELGDSSKTDTAKANRLKEDLLQQETLQNYGLKTSVKADESEAATLLRYSSILAKEGY
                     QPKDLRAQIEQAIVKSQDFKVFIKEGDTLRESLKRIANSDSANEFAAYLSGPGNFTKM
                     RQGLRGTSEFSSMVSRLLKSEKVDQKHKEQLLAELKQAVKSKEVESEFVFYSILKDNT
                     EEPLVKAWVDEIKTSTGAAQYPGMAQAMLWASAQLDRPTPEFEKLLKANKSESSEWIF
                     IANPKLSAENTTADRLIHSTFDRIIKNAKGMDDTKLLVWQWIEILDGKSMDKFGPTVV
                     KALPSLLTAKTVPVERLYHFVQKLVENEGANRELMQQTIDTLRPFIRNEKFLWRANDT
                     NIQTKKQFEEFESAGFDRGASILWKAYILLNDSKAGRKILKDHPVWQELKGLAKEKMR
                     VDIPVYSSSLTADSASIPEPMVPFYVGKVQEQLRTLTDSTQFMGQLDKFKTEIISSPE
                     LIAPVIKRMGDLKEGSEAYRYLGALIVDFAKRSQQNARFILSKLGAMNPTYYHDP"
BASE COUNT         6037 a         4634 c         5445 g         6043 t
ORIGIN      
        1 gagggcgcgc aggttcttga tctcatcgct ccctccacag gccagaggcc tgacatgatc
       61 aatttgcagt tgaaactcag agccacatct tttccctgtg agggggtggg tatattcaca
      121 gcagtgctgg gctttttcaa aaacagctct tctgattgta agcgaaagag cctttctttt
      181 ttgccggact gttttatagg gcctagacgg cgtttcgctg aagctgggtg ttaagctttc
      241 cccattttgt ttgctgaatt tttcaaaggc ctgctcagaa gcttggactt taacttcaga
      301 gtctttggat tttttagccc cctcgatttt tttaatataa gcttgagcta aataagtcat
      361 cacggcggcc actgtcatct ttttggggtt tcaccttctg ctgagttttc ggagtttgat
      421 tgagctcaga ggccaggatt ttcccggttt caaagacact tttattttca agctttccca
      481 aaagctcttg ggtggctttc acagaaagct ctttcccggc tttgcgttct tgtttaaggc
      541 tctgatcgat ctgcaccaat tgcgagagat tcagcgctcc ttcttcaatt ttgcgggcga
      601 tttcaggagt gtgtttgagc accagagccg cttgcatccg atcataggcc gccttttccc
      661 catagtagag gtgttgggtg agatacctat aaagagaggt aaaacccaga tctaaataaa
      721 gttttcgaac ttccacttcg ttaatatgcg aaagcaccag gtgagtgatt tttcgctccg
      781 tgcgcgaaag cctttcaaga cgaaaaagga tttcttgatt tgaaaccttt gagagatcca
      841 aggcgtattc aggggaaggt atcggcatag aggggctcct gtcaaaaaaa ggtataccac
      901 gagttttgag tgcggatttt tatgattttt ttcagaggtg aaaacttcat gaatagctct
      961 gcggaagggt ttgagagcgt ccaaggcttt cttggtgaag agttgcttta cgatccgaga
     1021 ggcctcgggt ggagaataaa aaaaccaagc catcgccttc tgagagagac gaaaaaccgt
     1081 tgcaagagat attttataaa aactaaatat atacgcaggg cttcatggaa ttgccgtgtt
     1141 ttaagagtta cctgaaaaaa tcgttgggaa atatttatca gaagagagga aatcttcgga
     1201 agagaagagg ctcgtagtga tgattatctt gcaaggggtc tgattcccct atccccacaa
     1261 attccagaaa gaggctcggc cccgacgaga acttcgacaa tcgcaacccc ggattgtcac
     1321 tcttgggcgg ggtctcagct tgaaacgctt attaagcatt ctattgcgat tggccggtat
     1381 ggaccttgcc ttctcattag agtgtggatg tgtaacttaa agaggtaaag aggtgctttg
     1441 tggaaactcc tgttaaatat cttatagtgt gccttttagt agtggcgagt ctagtggcat
     1501 gctcgaagaa aagtgacaat cgcgaggcga gggctccaga ggtgggactt ggcgtccctg
     1561 ggaatacgcc gacctgtact gctggtcaac aggctgtggg acgcatctat gagagtggta
     1621 acacagcttc taattcttct ctgacctttg aacagcgggt gaagggcttg ctttctgcga
     1681 ccgtggaccc tcagtttttt ggcaccatta gcggtagcgg aaatgatgct cagaacggag
     1741 tgacgatcga agggcgtctt cgttacgatg ccaatggcac cgttttattg gatcagtcga
     1801 atgtgaaaat catgatatat gattcctttg tggggcaatt ggattctcaa caacaagtga
     1861 ttcaagccta tccgattaat ttcaatgcgg ccgctgcggg caccatgaat ttacaaacca
     1921 aagtttttat gctgaaattt aaagacgaat ttggtgaaat cactttgaac ggaacagtca
     1981 atgctgcaaa tgtgtcaggg accatcagtt atcagaacga taaaaatgtc acaggcgggt
     2041 ccctctccgc tggctttttg ggagagttct ccatcgcgac ctgtggatgg attaactagc
     2101 ctgaagctct gcgttttcac ttgcatgcag atcctagtgg gggattaatc agagggatgc
     2161 ctcaaaagtt ttcatttgat ttagatatgt tgaattcagt cgcgggagga cgttccgcca
     2221 ttgatacccc acgactcaat atcaagtctc tcgagcaagc gacctccttt attcaaagct
     2281 atggttttga ttatagcaat gttcaagatc aggaaaaaat ttggtactat catcgtagag
     2341 ctctcgtgct gatcgttgaa aaattaggtt ttgatcttca ggagattccg gagcttttgc
     2401 gggagcgcaa ggccctggta gatatccgtc ggctcttgct tttggccagc tccggagaag
     2461 cctccgaacg cagtttgcag cgctggtcct gtgcgattct tcgctgcatg catgtttatg
     2521 ttcatgcgga aaatgatttg ttctcaagtt ttagtgaaga aattcaaagt cagattttga
     2581 ctcccttcca gaaagccatt tatcacgacg ggacaactca caaagcttat cttaaaagcc
     2641 tttcaaggga ggggcgggaa cctgtggagc ttctgggatt tgaggtaaag ccctttaaaa
     2701 catcggcgag cacggtgatc aaacttttgg ccaagcccga tgctttggcg ttgaaaatct
     2761 ttgataaatt aggagtgcgt tttataactc gttccctatt tgattccttc caagtgattc
     2821 gttttttagt cgaggaaaat atcatgagtt ttcctcacat tatgccggat cagtcttcga
     2881 acaatcttta tcctgttgaa ctctttgtca gtgtttgcaa agagctgttg gagtccggaa
     2941 agaaatacag tgagacggag ttgaagggca tttttgatga aaaattgcgt tctctgggag
     3001 agggagctca attttttcgt aaagagaatt tcttttccgg agaagactat cgtttcatta
     3061 aatttatcac ccgtaagttg attcatatac aaccccaggc tggcaaagaa ccttttagtt
     3121 ttttctatcc ttttgagatt caaattatgg atcaatcagc gcatcagtca attctttcgg
     3181 gaccctctga gcatatggct tataaagaac gccagcgact ggcggctcgc aaacgattat
     3241 tttcagagtg agaaagtcaa atatgagatt agtggcctct ttgcgtagta tccttatgac
     3301 cttgatttac cctttggtat tggttttttc ctcgattctc gtgatggcgg taaatctttt
     3361 ttttaataat aaaaagtggg acgattacat agtgcaatta tgggcccggt tcagctgttg
     3421 gatgtttggg gtgaaagtta aagtcagggg cgctgaacat atccctgcgg gcggctgtgt
     3481 ttttcttttt aatcacacca gtttttttga tatttttacc atgcaaggag ccttgaatgg
     3541 ctttcgtttc ggcgcaaaga tcgagctttt taaaattcct gtttttggtt tggcaatgag
     3601 gcgtctcgga gcgctgccca tcgcacgtca taaccgagac gaggtcttca aggtctatga
     3661 agcggcccaa gagagaattc atcgggggga gcgctttgcg cttgctccag aagggacgcg
     3721 tcaagaagag gagaagctcg ggccctttaa ggcgggaccc tttgtttttg ccatcaacgc
     3781 aaaagcgccc ttagtgccgg tggtcattcg tggcgctgct cagattttgc caaaagggtg
     3841 ctggattcca aatcttgatg cctggactcg ggaagtcact atcgatgtcc taaagcccgt
     3901 atccacagaa ggatatgata ttaaggatcg ccctgtcctt caggagaaaa tccacaaaca
     3961 aatggaagct tactttacca acgtccattc gtgactgagg atatgggccc gcatttttat
     4021 tttttgtgtg ccggtatcga tcaggatctg aagccgtgag tgagatatag ggtcaatctg
     4081 cgtgatgcga agatggcggg gagttttgtg gaggctgaac tgaatttgct cggcaagctt
     4141 gtcccatttg atataggtct cgaaactttt tccctcatca aagctgcggt aatttcccac
     4201 gtaccaaatc ccgtcaggat ggatggcgac aggaaagttt ttaccttcaa agagagcgat
     4261 ctctttccag attttcccgt cttcagtgcg aaaaataccg ttggctgaaa ccaagccttt
     4321 gatggacttt gcggcaaagg cgacggcact gacttcgcgt tttaaaagtt gctcactggt
     4381 gatttgattt tcagccagag gtttgtagga aatcgttggt tcatccatac gccaccacac
     4441 agaaccatgg ccttccaaga ggctgagagt ccagcggtgg gctttgttgt tggcaatttc
     4501 aacgcgagag cgaatggagg gagcttcctc tttcagtttt tggcagacca cgccgcggga
     4561 gacataaggg ttaaaggcga gaaagccttg cagtggaatt ttttgattgt ccacggtgaa
     4621 aagatattca ttttctcgat gagaaatccc cagccaacct ttgttttggt gataggccca
     4681 catggcaaag tctgcgcggc ctgtaagatt cgccagatcg agatatccta tatgtccttg
     4741 gtaagaaact ttgagataac ttttctcgat tcccaaagga gtcagtcgtt gtaagcgcgg
     4801 caccgtggtg atgattccgg aagagtcctg gggggttttt cttaaaaacg tatcaatcaa
     4861 agtgataaat acgccttggt cttccgccgg agcttcgagc aaatggagcg gagcccaacc
     4921 tctgagtttt gttttggtct ctaaaagctc agcccaatag ctgttggtgt gcagaacttg
     4981 cagagtggta tttgcactga cggtggtgat gcgcgatgag gtctcttgcg gatcctttaa
     5041 aaggtctaag ctttctttgg aaattaaatt tttcgtcact tgcacgtcgc ggatcactgt
     5101 ttcgccggga atttcataag ttttcttatt ccagctcacg cgcaaccaaa gttcataatc
     5161 ccggcgcagg accttctctt ccaggtcctc tcggctggct tgacctgagg gaaaaagact
     5221 ttgctgatta cgatagaaag aaagagccgg gatttctgcg gcgaagctcc aaacagaggt
     5281 gttaaggcta agaaaaacaa gaaaggagat tcgcatgaga ctttattctc tcaggagttg
     5341 cggacgactc caagaccagg atttttgttt tgacgccctc gggagctttt gaccaggctc
     5401 cgggggccgt atatacatag ggggattcgt atcgggttct ttatgctttg cgtaaagttt
     5461 catttcgcag tttcatttca caaggagagt ttatgggtat tgagggcaat aaacacattc
     5521 aggatgttcg cgctattggc gttgtgggag ctgggcaaat gggcaatgga attgcgcaag
     5581 tggcggctca gtttggttat cacgtggtga tggtcgacgt ggcaatggcc gctcttgaaa
     5641 aaggtctggc aactatttca ggatcttgtg accgtttgat taagaaggcc agcatgacag
     5701 aggcgcagaa gcacgaactt ctcgagagaa ttaaaacatc tcaggatatg gccgctctca
     5761 aagaatgtga tgtgattgtt gaggcagcca ctgaaaatgt ggatctcaag cttaaaattt
     5821 ttaaacaact tgatgaagtg gcaaaggcaa aggctatttt ggcttccaat acgtcttcca
     5881 tctccattac caaaatcgca gcggtcacca aacgtcccgc gcaagtggtg ggcatgcact
     5941 ttatgaatcc agttcccttg atgaagctcg tagagggcat cagaggcttg caaacttcgg
     6001 atgagacttt caagacagtg aaggcacttt cagaaaaaat ggaaaaggtt tttgttgaat
     6061 ccgtcaaaga catgccagga tttatcgtca accgaatttt gatgccgatg atcaacgaag
     6121 ctatttatac gcttcatgaa ggcatcgcca atgtcgagag tattgataat gcgatgaagt
     6181 tgggaacgaa tcaaccgatg ggacctttga ccttggccga tttcatcggt ttggacacgt
     6241 gcttggccat catgaacgtt ctccatgaag gtttaggaga ttctaaatat cgcccttgtc
     6301 cgcttttggt gaagtatgtg gaagcgggtt ggttgggtcg aaaaacaggt cacggcttct
     6361 acgattactc tcagaaataa ggattaaaaa aatatgtcac agttttctta taaagcaatt
     6421 aacttagagc ttaaggacaa tggggtctgg gtgttaacca ttcagcgccc tgaagctttg
     6481 aatgcgctca atgcgcaagt gttgaatgat atggccgatg ctcttcgcca aatcggagaa
     6541 atgaattttg aaaacacgcg cgctttaatt atcacgggca gcggcgaaaa ggcctttgtt
     6601 gctggagccg atattaaaga aatcgcggag ttggatgaag aacaagccat ggccttcgcg
     6661 caacggggcc aatctatttt tcatgaattg aatctgttaa aaattccggt cattgcggcg
     6721 gtcaatggtt ttgctttagg cggcggtttg gaattagctc tgggctgcga ttttatctat
     6781 gcggcggaag gggccaaatt cggtttgcca gaggtgagcc tcggcttgat tcctggcttt
     6841 ggcgggactg tgcgtttggc tcgggccgtg gggattcgca aggcccgaga gatgacttat
     6901 acgggtgaca tgatttccgc ggcagaagct ttgagcttgg gcttggtcac caaggtcgtg
     6961 ccgcaggcgg agcttatgag tacagtgatg aaggcagtaa atactatttt aagccgtggc
     7021 cctattgcag ttgcggcagc gaagaaatcc atcaatcaag cctatgaact cgatattgac
     7081 gaagcgcaaa aaaccgaggc tcaacacttc gcgtatttgt ttacgagcga agatgttcgt
     7141 gaaggaactc gtgcttttat tgaaaaaaga aaacctgctt tcaagggatg ttagaaccta
     7201 tggctactgc tccgaactat actccaaaaa atcctattcg tatcgttaca gccgccgctc
     7261 tttttgatgg tcatgatgct gcaatcaatg tcatgcgtag aattttgcag gagcaggggg
     7321 cagaggtcat tcatttaggc cacaatcgtt cggtgaacga tgtggtgaaa gcggttctgc
     7381 aggagggagc gcaaggggtg tgtatcagct cttaccaggg cggacatatg gagtatttca
     7441 aatacatgaa ggatcttctc gacatgaatg gcgccagcta cgtgaaaata tacggcggtg
     7501 gcggcggtgt gatcgtgcat gatgaaaaac acgagctcga agcctacggt atcgatcaga
     7561 tattccaccc tgatgatagt cgtcggttgg gccttgaggg catgattcaa gtgatcgtga
     7621 gggcttgcga ttttgatgtc accgcagcga aggtcaaggt gattgaacat aaagtggaaa
     7681 atcccttcaa agcggatagc attccttctt tcgatttggg acgggctctg accttcattg
     7741 aaaacggcgt tgttgaagaa gaagtgaata aacttcttca gggaagtgcc ttggatagat
     7801 ttcagactaa gttcaaaagc aaattcccga gcaaaaaaga aactccaccg gttttgggaa
     7861 tcaccgggac cggaggcgct ggaaaatcca gtttgatcga tgaaatggcg cagagatttc
     7921 tgaatgccta tccagagaaa aaaatcgcga ttgtctgtgt ggatccctcc aagagaaaaa
     7981 ccgggggatc gttgttaggg gatcgtatcc gtatgaactc gctgagccgt catcatattt
     8041 ttatgcgttc agtggcttcc cgtggatccg gtcgagagat cgcgtccagt ttgcctggaa
     8101 ttttgcaata tctgaaaaag tttgattttg atctgatcat cgctgagacc tcggggatcg
     8161 gtcaggggaa cacggccatc actgaggtca gtgatctttc catgtatgtg atgacttcgg
     8221 atttcggtgc gcagtctcag cttgaaaaaa tcgatatgat cgatttcgcg gatttgattg
     8281 cggtcaataa agccgatcga cgcggagctt cggatgcgct tcgtgatgtg actaaacaat
     8341 ataaacgttc ccgtaaaatc ttcgatgaaa aagcaaaggt gccggtcttt ttgacccagg
     8401 cttcacaatt taatgatggc ggcgtcaata aacttttctt taaactctct gagttgctcg
     8461 atgagaagta cacgcaaaat aactggaaga tggaggagag ctataaagcc aacattcttt
     8521 ccgcggaaga gcacggtatt atcccgcccg agcgccagaa ttatttggcc gagatcgttt
     8581 ccacgattca taaatacaaa gagcgcactg caaaactcgc ggaaaaagcg tcccttttag
     8641 gtgtgttaca taatgcttct gcactgctga attccccagc ggagaatgta aaaaaagccg
     8701 agaaaagcct cgaagaagat ttcaccaacg aagaactaca tagtctcagg gagtggaatg
     8761 aatttcagca aagatacgcc tgcgaagagt tggtgttcaa agtgcggggc aaagaaattc
     8821 gccaaccttt aaccactgaa agtttgagcg gcacaaaaat cagacgggtg gtattgccaa
     8881 aatttaaaga ctggggcgat cgctttagtt atttaaagct ggaaaatgtt cccggggagt
     8941 ttccctttac cgctggggtt ttccctttaa agcgcgccga tgaagatccg aagcgcatgt
     9001 ttgccggaga aggaacgcca gagcggacca atcgtcgctt tcattatttg accaagggtg
     9061 aaagcgctcc gcgtctgtct acggcctttg attccgtgac cttgtacggt caggatcctg
     9121 atccgcggcc agacatcttt ggtaaagtcg gggagtcggg agtgaatatt tgcaccctcg
     9181 acgatatgaa gaaactttat gcgggctatg atttgtgttc agcgaatacg agtgtgagta
     9241 tgaccatcaa tggtccagcc cccatgatct tggcttttta ttttaatacg gccattgatc
     9301 agcaggtgga gaagaaggaa aaaacactgg gccgtcagct ttcaccaagc gaatggcaag
     9361 aagttgctga ctggaccgtg caacaagtgc gtgggaccgt gcaggccgat atcttgaaag
     9421 aagatcaagg acaaaacacg tgtattttct ccatcaactt tgccctgaaa atgatggggg
     9481 atattcaaga gttcttcgtg cgtaagaatg tgaagaactt ctattccgtc agtatttcgg
     9541 gttaccatat cgcggaagct ggagcgaatc cgatcagtca gctggccttc accctgagca
     9601 atggcttcac tttcgtggag tattacttgt cgcgcggttt gaatatcgac gatttcgccc
     9661 cgaatctgtc tttctttttt agtaatggtc ttgatcctga gtatgcggtt ttaggacggg
     9721 tggctcgccg tatctgggcg attgcgatgc gagatctgta caaaggcaac gatcgctcgc
     9781 aaaagctcaa ataccatatt caaacctctg gccgttcgtt gcacgctcaa gaaatcgatt
     9841 ttaacgacat ccgtaccacc ttacaggctt tgctggctct ttatgataac tgcaacagcc
     9901 ttcacaccaa cgcctacgat gaggccatca cgacgccgac agaggagtcc gttcgtcggg
     9961 ccatggcgat tcagcttatt atcaaccgtg agtttggtgt cagtaaaaac gaaaatccca
    10021 atcaaggcag tttcttctta gaggaattga cggatttgct ggaggaagcg gtcctcagtg
    10081 aattcaaacg catcaatgag cgcggaggag ttcttggagc catggagact caataccaac
    10141 gctctcgtat ccaagaggaa tctttgtact acgaaagatt aaaacatgat gggacttttc
    10201 cgattatagg tgtgaacact ttcatagatc caaggactct tcagtccgat tatgttcgtc
    10261 ccaaaatcga gttggctcga gcttcctacg aagaaaaaaa tcagcaattg gagaatgtgc
    10321 gtaatttcca aaaagaagag cggactcaag ccgcgcagga gttgaaaaat ctcaaagcgg
    10381 ccgtactcaa aggagagaat atcttcgtgg ctttgatgaa ggcggcccgc cactgcagtt
    10441 tgtatcaaat gacagaggct ttgtacgaag tgggtgggca atatcggagg aatttataag
    10501 cgcgtgatgt caaattgcac tgagctacgc aaaaaagaga acaggagcac tcagcgagcg
    10561 ttcatcaata acatgaagcc gttttttatt atgtagaaat aagacccgcg cattttcgta
    10621 tttctttaaa aaagaagctg cagaagactt ttcagatcta ttagggagct cctcttctat
    10681 aggaagcata cccagtcgtt ttccaccttg ttctatgcaa aaagggaggc gcgctccgcc
    10741 acgggttaga aaatgaaatt ctcgagtcgt agaccctaat tgataatacc actgcgttct
    10801 taagtttcta taagcgagct gctcgagctg atccttctca gtttgctgac catcaagcat
    10861 aaaaaaagac tctgcctgat cttccaagta aaaaatatct ccttttttgg taccttcaat
    10921 tctgagtcgg cgtataagaa aaatgccttc aaaggcctgc aataatttct tttgtgtgac
    10981 tggggacaag ccaattgctt tttgtgccgc tgaatagtta aaatcctgtc cttgattctg
    11041 tgctatgtaa cgaaaaaaat cttgaatttg cgaaaacgat aaatttgtcg gatagaccaa
    11101 cctaacatcg cgatctaata aagtttgtaa ttgctcctta attcgaagat gtcggaggtt
    11161 cttattttgg ataaagcaaa ctcctggcaa tccaccgtgc tcgaagtagt aattgtattg
    11221 tttttcgagg ccgctaattt tactgttcga cagagattgt gctactcggt ccatcgtaga
    11281 tagatttaaa attgtcgtaa tagtttgtgg aagctcccga tgagccaact cactaattga
    11341 aaaaggaaga agttcaaaat tcactattcg tcctgttaaa gattcgcgaa tagcctgacg
    11401 actggtgaaa cgaacgctcc ctgagagaag aaactgtcca ggggatttgt ggatgcgcac
    11461 aaattctttg agagcgggaa ataaggcggg agccaattga cactcgtcaa taccttggag
    11521 gctttttggg taatacttaa gaaaattttc tgtctcaaat tgagccttgt tgagttcagt
    11581 ttttttatcc aaggttctgt aggtatcgca cattttctca aggactgttg tttttccaac
    11641 ttgtcgatgc cctaaaattc caacgagtgg actaagatga accgcctttt ctatcatatt
    11701 ttgaatatat cgcactctta aatgagccat accatctccg ggtgtaatag atctagtata
    11761 aagattttta tcggtattgt caattttaca tccgatgtaa aattgagaaa ggctaatatg
    11821 atattacatc ggatgtaaaa gtggaaaatg gtacaagact cagtcagccc tctttgacca
    11881 tcatctcttt agagatcctc ctgtcagcgt gggtgatggc gtgataacat cttgagattt
    11941 tgactgattc gaagtaggct tctattaaat cttcttcgaa aaggaaatgt tatgaagttc
    12001 attttttgtc tcttcacttt tattttgttt ttgagcgaat ttgactcagc cttcgccacc
    12061 gaatctgaat atatcgaaat tcatgaagct ttttatccgc gaatcgaatt gacccttgcg
    12121 ggaggcttgg cgctgcaaac cggttatctg acaacaaaag cttccctagg cacctatatc
    12181 tttggagtga actttgtgat gcctggcggt ttaatgtctt ttgactggca gggatttcat
    12241 gccgatcacg atacggggga gttccctctc aataataata aaagtgtgac cgtttccacc
    12301 ttctctttta ttccccatgt ccgggttttt aaccatgagg catggaatgc ttatgtcggt
    12361 cttggcttta cgcaagtgag tctttatcag acaagtccgg agtatatggt ggattacggg
    12421 agttttgtat tctccggatt gctgcgctac gagctgaata ataagtggtc tgtgttgtat
    12481 aaaactcaat ggtacaatgt gaataaaact gaaaacgatc agaagaccag ctttgaagtt
    12541 tggaatcacg ctatcggagc gggatacagt tttttctaga aaaatagatg taaagtgctg
    12601 attctgggat gggagaaaaa acttaggact ttggaagtta aataaaaaac cgttaagcac
    12661 tgttaatccc atagaaactg ctaaagcctt agttctgaag ctgaacgaca agatcaaggt
    12721 tgagaaggct tttttgtttg gttcaggggc cagaggtgag cttacgcctg attctgattt
    12781 agatattttg gtagttctta agaatattga agatatgaag gccgcttaca aagttgttgg
    12841 gcagaatctt ttttctccga ttgctgtcga ttggatattt aaggacgcaa aaagttttga
    12901 agagagaaaa gattttggtg gggtttgctt cgaagcattt catcatggag ttcaaatata
    12961 tggtcaataa ttcaaaaaga ttgtttaaaa aagagtttgt aaagtgggcg taggatataa
    13021 ttaagggata ggctcagaag aaaaagatat aagaaaaaag ccaatcaggg gtattctggc
    13081 ggtttctgta ttcaagccga aattcctggg ctttggagga gtaagaaact ccccactgct
    13141 gatgccatag aaagcctccg gattgataag cataaagata gcggacgtcc aagagggagc
    13201 gaaagttctg agtccactgg gtgataagga tgttttccac tcccaaatac ccggcgtagt
    13261 tttcgttttc agtcctattt tcactgcgcc ccagaagcac ccagcgggcg gggattttta
    13321 aagaaagatc aaaactggtg ccgcctccaa aatccaaata aggcactagc tttggtctcg
    13381 ttcctaggtc gagagtccaa ctgagcgggt gatctaatat atttgtgggc gaagtggcca
    13441 agacatttaa aaaagtccaa tgagcaaggt cgagattctt ttgttgtgaa aaataacgaa
    13501 attcaaagct caaggtttcc aaatgaaaaa atggcgccag gcctgtgtca tgtgaaagca
    13561 gatcgtgaaa agcgcggcgg aatttaaaat ccagataatt gtgatggttt tcctgtccgt
    13621 aaccgaaata cagggccttg gggttctgac tgagcagggg ggagctcggt tttggaatac
    13681 tcactggctc ggtgataagg cctaattttg accgttgaac cgccaaggca tacttatcgt
    13741 ccttaagttc tttttgctct cgaaactctt tgatggataa gtagctcaag ctggcttcca
    13801 gagtctcggc tttctcttta ttggtgagct ccgcagtgaa gtgaaataac tgaggtgatt
    13861 ttgtgatgtt cgtgagttcc tctttctgct ggagattcag tccggcataa cgactgcgcc
    13921 actccgcttg caaagaagaa cggctttttt catccgctag aagatcctgc cgcgcaagaa
    13981 ccttcagagt gtcgagggga atgacaaagt catgaaagct ttgggctaga tccagttgag
    14041 gccgcaaaac attgagaagt tctaaaattt ggtaagagca attttcgtca gcgaaatagt
    14101 agtaagaaaa gctgccatcg agttccaaga ggtgatcgat caaaaactca atgtcgtcag
    14161 tggacaagct taatttgtat tcccaaagat ctcttccctc gagattgatg tattcgcgga
    14221 ttttctgatg ataaggcaac atcgagtagt tcccaggata aaaccccaac aaccccttaa
    14281 gcgcaaagag ggctccggag tcatgacccg tcagggccgc atagttgata ccataatcca
    14341 gcaattccag ttcatgcaaa tgatcggggt tatggagcct gaggaaagta tgaccaaagc
    14401 ttgaaccggg gctatttaga tcactggagg caaaaacaat ataagcttcc tcggcgccga
    14461 gttgcttttt ccattggtcc cgatcggggc agcccctcac atcttccgcg gcaaggggaa
    14521 ggacagattt gagccaacga gtgcgtgcaa gatagcggca ttgaggtgaa ggtgatgaag
    14581 gagctgaaaa caaagtatca atagttgcca agagttccgc ttggggattg tccgcgccgt
    14641 cctgggcaat aaaaaaattt cctttgagtt gcgagtgata agtgccatac caggatttgt
    14701 gatagtggcc aagttttaac cattgaggat tttcgaagag tttttgcgcc agaatgattt
    14761 ttttatattc ctgcgtgcgc tctgaagaaa agccccatac ccagcatggg gcgaataaaa
    14821 atattaagat ccagttatgt accacggcaa gcttgtgaaa cttcggagtg tcccgccatc
    14881 tcagtattta aattctgaac catatccaca gggcttgtat tggcactggg aagaatatgg
    14941 gaataagaag actgcgtgaa ctgagcaaag gtcttttgag aatcctgaga agtgcaacct
    15001 tttaggagag ccaaggtcga aagtttttca ccgtgacctt gggccatttc ccgtgaaagc
    15061 tcttcttgat tgatttccac gaagtactgg atttgtttgt cattcataac aataccactc
    15121 gaagagcaac ctgaagtccc gaaggtgatg cccaagggtt gagtaaataa gaacgaattc
    15181 gtggtcattg caaaaagctg taaaagcttt gcatttttgg tgaaaataat tccacctaga
    15241 ccgcaacccg catctcccgc aaaagcggat gggcttaaaa aacacgcgct tgatagtaaa
    15301 gcaatcccca atagtttttt cattgaaacc tccttgtatt caattaaaaa atgaatgtgg
    15361 ccttataatt tgaccttatg ctgacttaat tagcggcaga gatcaaagat tttctcagcg
    15421 atgcgcactc catcacaggc cgccgatgtg atgccgccag cataaccggc gccttctccg
    15481 gcgggaaaga gtccttggtg actcaagctc tgaaggcttt cctcattccg ggtcacacga
    15541 agaggacaac tggtccttga ttccacacca tagagttgag actcctcagt gataaagcct
    15601 ttcatagagc gctcgaactt attgaagcct tctaagatcc gtttatgaat aaagctaggg
    15661 agtaattcat ccaagcgcac cgccttggtc cctgaaggac aagagccagg atgaacttgg
    15721 ccgatacgac cgcaaataaa atccatcatg ttttgcgccg ggagttcttt gctgccgccg
    15781 accgctaaaa ccgattggta agcttgggtt tccaattgtc gtcgcagttt gagaccgcca
    15841 aagatatctt ttccaaaatt cttttcatga tccacgctga ccacaatggc ggcgttggca
    15901 aaaggtgaat tgcgattgta gttactcata ccattgcaaa cgatgccctc agcctcggtg
    15961 ccactggaga gcacataacc cccagggcac atacagaaac tataaactcc aatattggtt
    16021 ttatgatcat gatgggcgag tttgtaattt gcagaaccca actttggatg ttcggaaaaa
    16081 tcacgatatt gaattttatt tatggccttc tgcggatgct caatacgcag tcccatggca
    16141 aaggatttgc cttcgataaa gacttctttt tccgctaaat gcaggagcat atcttcggct
    16201 gaatgtcccg ttgctaaaac cacaaagaga gagcgaatct ccgtcccatg ttcagttttg
    16261 acgcccacag tttgtttgtt ttcaaagagg atttccgtaa cctgagtatt gaaatggatc
    16321 tcacagccgt tggcacgtaa aaattcgcgc atcttaggaa tcacccgacg aattttatcg
    16381 gaccctacgt gcggattcgc gagccattga atttcaaccg gtgctccgaa ttgcaccaga
    16441 cgatttaaga catagggaat atgcggagat ttaattcgtg tgattaattt tccgtcagaa
    16501 taaagccctg cgcccccttc gccgtagcag acattgttgc gacgatcgag ttccccatag
    16561 cgccagtagc ggttgatgcc cttgatgcgc tccgaactct cggaaccccg ttcaaaaaga
    16621 atgcagggga tgccacgctc cacaagtcgc aaagccgcga aaagaccggc tggacccgag
    16681 cccacaatca agggtttttc cgtgaccctt ttatggatct tttccacggg gaaattgtca
    16741 attttcaggg tctcatttgt ttccgccact tccacagtat aaacgaagtg aacgttacgg
    16801 gatcggcgcg catccacgct ttggcgcagg atgcggtaag gcccatgatc tggcatcagg
    16861 tactgtaatt tttcctcgag atccgcatcg atcggcactt cgagattctg gaaaatccgt
    16921 gacataggtt aggtatagca tcacaataag ccccatgtca gccattacaa aatttctccc
    16981 tcgcaatcat ctcagttatt ttatcggcaa gctcgttcac tggaaggggc cgcggatttg
    17041 ggccgaattc accattcggc tttttgctaa agtttatggc attaacttga aggaagctga
    17101 aaagcccgtc agcgggtatc ccagcctggg ggagtttttt attcgtcgct taaagcccgg
    17161 ggctcgcccg ttggctgagg ccgcagccgt tcacccggtc gatggccata tcagccaatc
    17221 gggccgtatc cacaacggtc ggctgattca agccaagggg aaggagtact tcctacaaga
    17281 tttgttggct cattccgagg ccacaaaaac ctatcaaaat ggatttttta tcacctacta
    17341 tttatgcccc accgactatc accgcgtgca tgcccctgtc agtggttgga ttcgaaaagt
    17401 cacccatgtg ccaggggagt tgtggccggt gaataaatgg agtgtggaaa atgtccatga
    17461 tctcatgtcc gtcaacgagc gcattattgt ggaaatggcc actgactttg gcccgacgag
    17521 cgtcgtgttg gtgggggcga ccaatgtggg gcaaattgaa ttgagtttcg atagcggctt
    17581 tcgcggtaat caactcactt tatcgaaaaa cagggtggtg gagtacaaga ctccgattcc
    17641 cattgaaaaa ggccaagaat tagggatgtt ccgtatgggg agcaccgtga tcatggttta
    17701 tgccgaagag tttgcgaacc gcttcgcagg aaaaatgaat ctcgaccttt acgtgaaagt
    17761 aaatcaagct ctgacaaaat aaactttcag ctaaatctct taggccaccg gataagagca
    17821 ttcgttggtg ggtgtttcag agaggctcac ccgcaccaac atgggtagta cctggcgggc
    17881 tttgtcgtaa acccatttcg ccaaaagctc tgaggtggga ttttcaagcc cggggacttc
    17941 atttaagacg tgatgatcaa gcaagctcaa aataggtttc attttcgctt ggatctcatt
    18001 gtaatcgatg acccagccca aatgggggtc gaggtcgtcg atcaaagtca ggacgatttt
    18061 aaaactgtgt ccatgcattc gtgagcaggg atggtttttc tcaagctttg gtaaaaagcg
    18121 agcggattca atttgaaaat gctgtttgag ttcgaatttc atgctataga tgataatact
    18181 atgtctacaa ccagcttaca attggcaaaa caaaatttta aattttcctc cgcgcacttt
    18241 ttgatctttg atgagaactc tgccgagcgt ttgcacggcc ataattatca agtgcgggtt
    18301 gaaatcggta ttcctgatcc agcggccatc aagggcacag gcttttttgt cgatttcaat
    18361 atcttcaaga aatacattaa gtccgccttg gatcgctggg atgaaagggt cctccttccg
    18421 aaagatcatc cagagatgaa gatccaggtc aaaggggccg ttcttgaagt gaattttcgg
    18481 gatcgttttt acgcttttcc aaaaaatgaa gtggtgttgt tgccaatcac taacaccagc
    18541 gttgaacagc tctccaggct cttagcggaa gacttcttaa aagaattcag cagccacagc
    18601 gtgagcttta tcagcgtgta tgtggaagag actccagggc agggcgcgac gacgaatgtg
    18661 aagtcgtcgc tgaggttcta aggaaattag tctgacaatt gcggagtgta gcgagttata
    18721 gacataagac ctggtctttg agtgtcgcta atgacatttt attcaatcct atcgcatact
    18781 aaatgatatg cgattgattt tattcctggg gttgacggcg ccatttttta gttttgagtt
    18841 tgcatatgcg catacaaagc ctgttgatga gcagattcaa gaggtcaaag aagttattca
    18901 gcctagggct ccgttcccta aaacaccggc cgagtcgacg gaaggagctg tcggctgctt
    18961 ggagtgctta gggataactg tcggcacttc tcgtcctcct gctaaatgcg atcttaaggg
    19021 ggcacgtgac gatctcaaac tgttgcccaa agattggggc ttaaaagcga cgggcgcaga
    19081 tttacttcgc gatgaacctc tgatgaaaaa cctcaagtac gaccatgcgt tgggaattgt
    19141 agatactgga cttaaactcc gaggtaatcc ggaggccggt caaaagttaa caagcacctc
    19201 cccggaagaa gacgccggaa tttttcacgg aagccatgta actggaattg cggtatctga
    19261 tgcgcatgga gtgaatccgt atcttgcaac caaagtcttt cctgtaggta cgaacatggg
    19321 gggcaagacc actaagagtg aagatttact taattcactt gaaaaagctg tggacgataa
    19381 gtctactttg gcgccccctc ctctttctca aattttggtc ccgatgtgag ggtttatgcg
    19441 ccgggctcca ggatcaaatc cttaaatgga gcctataatc cgaattattt ttcgacaaaa
    19501 tcatcggtaa tgatggatgg aacgagcatg gcggctcccc acttcgccgc ggttctgacg
    19561 actctttcaa atcttgctcc tgagcttgat gcggcagcta tagggcaact tattaaatcc
    19621 actgctatta atcgtggaac tggacaaaaa ccatttttat tcctcaatgc gtacttggca
    19681 tttcagacat tagcaaatgc tcgagactgc cttaagactt ctaagtcagc aggtcagtgt
    19741 ttgacggaaa gccaacaagc tgtgcagcag gccgccgtat taccgcaagt tccaaaaggg
    19801 acagcgtgca acgattggca gctctattac acggccttgc gaaaagtata ttttttgact
    19861 cagggaaata ggggagttgt aaaagcaatt gctgagctct tcgctggtga agataagccc
    19921 atcgctgaga aatttctatt cttgaatcgc gacagtcatg aggttcagag ttccttactg
    19981 aaagaccata caattcccga actttataaa gaggaacaaa cgcgggccat ggatttcttg
    20041 accggcaagc tcgatcttga aaaggtcctt gctggttacg atccatctaa gtatggaaga
    20101 gatgacttcg aaaattacac ggacggcaaa gaagcgccta aaaataaaca tcctttagag
    20161 ctactgctgc ccattaaaga aaattttggc gattttttga aatactgtga agccgatttg
    20221 aggtctgaaa aaagtcaggg accctgtcgt gactatgtag gcaatgcccc acgccagttc
    20281 aaggaaacgc ttattgaccg tcttttgcaa agagatccgc catccacaaa ttcctcgatt
    20341 ttgaatggcc tcaactttgg ttacgacaag gcttatgatg agaaagtgca ggggctttat
    20401 cagaaagcag ttaagatctg tttgcaagga tccttatgca aacaaaatga tctggcatca
    20461 ttcttcagta gctcttttgt tacaagagag caaaaggaaa ggttaagtca gacgatcctt
    20521 gcccgcgcaa aaaaaggtga ctttggtgat gatctaagct caggacaaaa ggatgcattc
    20581 atcacttact ggcgaacaat gacagagctt ggtgattcct ctaaaaccga tacagcgaaa
    20641 gcgaaccgat tgaaagaaga tttgctccag caagagactc tacaaaacta tggattgaag
    20701 acttcggtga aagcggacga gtcagaggcc gctactctct tgcgctattc ttcaatactt
    20761 gcaaaagagg gctatcagcc caaggatctt agggcgcaaa tcgaacaagc aatcgtcaaa
    20821 tctcaagact ttaaagtttt tattaaagaa ggcgacacgc ttcgtgaatc gctcaaacga
    20881 attgcaaatt cagattcggc caatgaattc gcggcctatt tgtctgggcc aggcaacttc
    20941 actaagatgc gccagggtct ccgaggtacc agtgaatttt catcaatggt ctcaagactg
    21001 ttaaaaagtg aaaaggtgga ccagaaacat aaagaacaat tgttggctga actaaagcag
    21061 gccgtaaaga gcaaagaggt ggaaagcgaa ttcgtttttt attctatttt aaaggacaat
    21121 accgaggagc cattagtaaa ggcatgggtt gatgaaatta aaacttcaac tggtgcggca
    21181 caatatccag ggatggctca agcgatgtta tgggcatctg cacaactgga tcgtccaacg
    21241 cccgagtttg aaaagttgct aaaagccaat aaatcagagt cgtcagaatg gatttttatt
    21301 gcgaatccaa aactatcggc agaaaacaca acggctgatc gacttattca cagcaccttt
    21361 gatcgtatta ttaaaaatgc taagggaatg gatgatacaa aattacttgt ttggcagtgg
    21421 atcgaaattc ttgatggaaa atcaatggac aaatttggtc caacagtcgt caaagcgctc
    21481 cccagcctcc tgacggccaa aacagttcct gtggaacgtc tttatcactt tgtgcaaaag
    21541 cttgtcgaga acgaaggtgc gaaccgagag ttgatgcaac aaaccattga tacgttgaga
    21601 ccctttatac gtaacgagaa gtttctatgg agagcaaatg atacgaatat tcaaacgaag
    21661 aagcagtttg aagagtttga atccgctggt tttgatcggg gcgcatctat cttatggaag
    21721 gcttatattt tgctaaatga ttcgaaagcg ggaagaaaaa tattgaaaga ccacccagtt
    21781 tggcaagaac ttaaggggct tgcgaaagag aaaatgcgtg ttgatatccc agtatattct
    21841 agttccctca ctgcagattc tgcatctatt cctgaaccca tggttccttt ttatgttgga
    21901 aaagttcaag aacagcttag aacattgaca gatagcaccc aattcatggg tcagctggac
    21961 aagtttaaaa cagaaattat cagtagtccg gaattaatcg cacctgtaat aaagcgcatg
    22021 ggcgatctaa aggagggctc tgaggcctat cgctatcttg gagctttgat cgtggatttt
    22081 gcaaaaagga gccaacagaa tgcgcgcttt attttgagca agttgggcgc catgaaccca
    22141 acgtattatc atgatccgt
//
LOCUS       DATBTE010000400         3103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1627206, whole genome shotgun sequence.
ACCESSION   DATBTE010000400 DATBTE010000000
VERSION     DATBTE010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627206"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /gene="raiA"
                     /locus_tag="VIG33_15440"
     CDS             complement(<1..265)
                     /gene="raiA"
                     /locus_tag="VIG33_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEY8272284.1"
                     /translation="MKLNYTFKHLDHSDSLVNYTEEKMDEIGKFLLKEGYGNVYYSKT
                     KSEFCVEVTVNTREKYFKATCYNLDIYTAVDLVVEKLEKQFIKT"
     gene            complement(692..2242)
                     /locus_tag="VIG33_15445"
     CDS             complement(692..2242)
                     /locus_tag="VIG33_15445"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY8272285.1"
                     /translation="MRDWITIGQLAKKTGLTARALRFYEAKGLLKSHYRGDNDYRFYS
                     KEEVDHALQIKEFRDLGFSLSEIAELLGQDPQLTTTNLSISLIRKVESLQGDQFLLKE
                     RIRKLEALIASLTTVHKLSEPQRRIVMEELVTQAAEKIKARGFKISEDQEGLLRSEVQ
                     ESQTRHFTKMMSLLDIIQSIAAEKGFKIGPGRGTAAASLLLYSKGFNHNQPEKFDLLP
                     ELFYHSLKPSLWIDVEYSAGQDFLAQLLQKTTLEELREAQIFFFQCPFLTVLSQVEKS
                     VGGINFDAISDDDELVLQSFWNDRVEDIFLFDAPEKSVMHANSDLNFWQKENELKRYI
                     WKLLKTYKVKGAEDILALCTLSFPVGNKKEMLEQYVTHEEIVPQAKNLPSEVLKLLQK
                     TKGLLIYREDFIRIVRHYTNWSVAECNAFYSFKIGRIEEFAKVSEYNDAIPSGVRELL
                     DREAKSVFLKSHMVCMWWFIKRSAILNSLYPDEYQAALKNWRRTYHSAWSDLGFIDKD
                     YRPLALYF"
     gene            2405..2851
                     /locus_tag="VIG33_15450"
     CDS             2405..2851
                     /locus_tag="VIG33_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272286.1"
                     /translation="MSKLILAATFMICIFGQGQAQAAELDPSNLVGEYKVQADLGFQK
                     YYLNFHVINTQEFEMQRTYPNGQVDEICNGTYNMNSQLLWTFTALTTSHVFKGRGSCP
                     SNRSHQVDFNIDFKNKTTEDLVNGTTVAITTSLAPGYVINSYFKRK"
     gene            complement(2875..>3103)
                     /locus_tag="VIG33_15455"
     CDS             complement(2875..>3103)
                     /locus_tag="VIG33_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272287.1"
                     /translation="SGQNGFESLQTSARYFAFSLGNIFAATLMTEFALHTKDLRDIES
                     ARRWCTRNTLFEVPETSASHRFSNSEIIRGL"
BASE COUNT          878 a          682 c          644 g          899 t
ORIGIN      
        1 gagttttaat gaattgcttt tcgagttttt ccacgaccag atccacagcc gtataaatgt
       61 ctaaattata acaagtggcc ttaaaatatt tctctcgagt attgaccgta acctccacac
      121 agaattcgct tttagtcttg ctgtaataaa cattgccgta gccctcttta agcagaaact
      181 tcccgatctc gtccattttc tcctcggtgt aattaaccag tgagtctgaa tgatcgagat
      241 gcttaaaggt gtaattgagt ttcatgtcgt ctcctctata ctgtgaggtc ccattgcgag
      301 accttggtct tgcattctta tcggcgaggc ccacctaaag atgagtgtgt ttgagtgaaa
      361 tttaattaga attttccagt gttttaaata atgctgcgcc tcctgttatg agttgagacc
      421 tcagggggga tcttctctta ctgagacaga tctggacctt aaaaaaggtt cataatttat
      481 acaaaatcga gtgtattatg aagacattgg ggacactgat ggctgcactc gcacatgttt
      541 taataatttt attacgaggg accgtggcgg aagccctcct tctggggcaa tctgcctggt
      601 gtattgcctt accctatcga tttccgaaat attcctaaaa gcggagagaa tcctgttaaa
      661 aattcgaagt agattctctc gacgcaggag cctaaaaata gagagccaaa gggcggtaat
      721 ccttatcaat aaagccaaga tcagaccatg ctgagtggta agttcgccgc caattcttca
      781 atgctgcctg atattcgtca ggataaaggg agttcagaat tgcactgcgc ttgatgaacc
      841 accacataca aaccatatgg ctttttaaaa atacactctt agcttctcga tctaaaagtt
      901 ctcgaacacc tgagggaatg gcgtcattgt actccgatac tttcgcaaat tcctcaattc
      961 ttccgatttt aaaagaatag aatgcattac actcagccac agaccagttt gtatagtgtc
     1021 gaacaatgcg aatgaaatcc tcacgataaa taagaagtcc cttggttttc tgcaaaagtt
     1081 ttaggacttc cgaaggtaaa ttcttagcct gaggaacaat ttcttcgtgg gtcacatatt
     1141 gttcgagcat ctccttttta tttccaaccg gaaagctcaa ggtacagagc gccaaaatat
     1201 cctcagctcc cttcactttg tatgttttca agagtttcca aatgtatctt ttgagctcat
     1261 tttctttttg ccaaaaattc aaatcagagt tggcatgcat gacggatttt tctggagcgt
     1321 caaacaggaa gatatcttcc acccggtcgt tccaaaagga ctgcaaaaca agttcatcgt
     1381 catcagaaat ggcatcgaaa ttaatccctc ctacggactt ttcgacttgt gacagcacgg
     1441 tcaagaaggg gcattgaaag aaaaaaatct gggcttcgcg aagctcctcc aatgtcgtct
     1501 tctgtaacag ttgagctaaa aaatcctgtc cggctgagta ctccacatct atccaaagcg
     1561 acggcttaag gctgtgatag aaaagctccg gtagcaaatc aaacttttca ggttgattat
     1621 gattgaagcc ttttgagtaa agcagcagac tcgctgccgc tgtcccacgg ccgggtccaa
     1681 ttttaaaacc cttttcagcg gctattgatt gaataatatc gagaagggac atcattttcg
     1741 taaaatgtcg agtctgagac tcttgaactt cgcttctaag tagaccttct tgatcctctg
     1801 aaatcttaaa acctctggct ttaatttttt ccgccgcttg ggtgacaagc tcttccataa
     1861 caatcctcct ttgaggttca ctcaatttgt gaaccgtggt taaggaggca ataagggctt
     1921 caagctttcg gattcgctct ttaagaagaa attgatcgcc ctgcagactt tcaactttcc
     1981 ttatcaagga aatgcttaaa ttcgtcgttg tcagttgggg atcctgtccg agaagttccg
     2041 caatctcact taatgaaaat cccaggtctc gaaactcttt aatctgaagg gcgtggtcta
     2101 cttcctcttt tgaataaaaa cgatagtcat tatcgcctcg ataatgcgac ttcaacagac
     2161 ccttcgcttc gtaaaagcga agggctcgtg cggtgagccc cgtctttttt gccaattgtc
     2221 cgatggttat ccagtccctc atcattgcct ccaaacacag cctgagggtt aacgtagagt
     2281 caaggtcaat ggcccttagc acacatttgc ttcgcgttaa tttgattctt cccacttctg
     2341 ttctgaactg attggaaaat cgtttagccc cctcttagag tgacttttca aaaaggagga
     2401 aaaaatgagc aaattgattt tagcggctac gtttatgatt tgcatttttg gacaagggca
     2461 agctcaagct gccgagttag atcccagcaa tttagtaggt gagtataaag tccaagctga
     2521 tttgggcttt caaaaatatt atttaaattt ccacgtgatc aatactcaag agtttgaaat
     2581 gcaaagaact tatccgaatg gtcaagtgga cgaaatctgc aatggcacct acaatatgaa
     2641 ttcccaactt ttatggacct tcacggctct gacgacgagc catgttttca agggaagagg
     2701 ctcgtgccct agcaatcgtt cgcaccaggt tgatttcaat atcgacttca aaaacaagac
     2761 cactgaagat ctagtcaatg gaaccactgt ggcgataaca acttctttgg ctccgggcta
     2821 tgttattaat tcttatttca agaggaaata acaaaagaga ttgtaaacca accgctagag
     2881 gcccctgata atttctgaat tggaaaagcg atgactggcg gaggtctctg gaacttcgaa
     2941 aagagtattg cgcgtgcacc aacggcgagc gctctcaata tcacgaaggt ctttggtgtg
     3001 aagtgcaaac tctgtcatca acgtggccgc aaaaatattg cccaagctga aggcgaaata
     3061 acgggcggaa gtttgtaaac tctcgaaacc gttttggcca gaa
//
LOCUS       DATBTE010000401         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_942694, whole genome shotgun sequence.
ACCESSION   DATBTE010000401 DATBTE010000000
VERSION     DATBTE010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942694"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VIG33_15460"
     CDS             complement(<1..809)
                     /locus_tag="VIG33_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272288.1"
                     /translation="MRRKNRKNILLFAIAAFWIAYVGIVSAEYYFNIASRVDQVIQNR
                     KKDVDFVLEFSGNLIIKDNLDSLTERLKQAREFRMVDFYLLQKKNKVITFYNKQNNPD
                     ELIQEHVNFNQFVESDDITFKTIQIYDYRLTVGVNTAKKSLSRVALGEQKYLILHNLA
                     MASAFAAMLFLFIYKDALSFTKLRGLQKVEPEVHLKSVPATKSVSSLAPALLHEMKSN
                     TFAPSNFDSTVVRIDLSDKTQALLSKSDYLSAILNKYQGAAHELISRYGGLL"
     gene            1203..>1783
                     /locus_tag="VIG33_15465"
     CDS             1203..>1783
                     /locus_tag="VIG33_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272289.1"
                     /translation="MKLITNHRGTSLVTVLMAVGIGAIVLAGITTMITDVFKSQRTAQ
                     SKEAHRELTANIRQLLTDPAICSVSFKGSNPSGKGYTKTQIVDAATPSNVKYQTGVDY
                     LNHLVTITGFEVRGFVADNPPVNDKIGKAELNIKMNKLGSTIGSPQMTTTVFLQATLD
                     ASYNLNSCYALGLADSLWQISPANMADIYYPSG"
BASE COUNT          529 a          371 c          351 g          532 t
ORIGIN      
        1 aggaggcctc catagcgact tatcagttca tgagcggcgc cctgatattt attaagtatg
       61 gcactcaaat aatcactttt actcagaaga gcttgagttt tatcgctcaa atcaatgcgc
      121 acgacagtgc tgtcaaaatt agagggagca aaagtgttgc tcttcatttc atgcaacagg
      181 gcgggggcta aagaggaaac agacttagtc gcgggcaccg acttcaaatg aacctcgggc
      241 tccacttttt gtaagccgcg aagctttgta aaactcaaag cgtctttata tataaaaaga
      301 aagagcatag cagcgaaagc gctcgccatt gccaaattat gaagtatgag atatttttgc
      361 tcgcctaaag ccacacgtga aagtgatttt ttcgcggtat ttactcccac tgtcaggcga
      421 tagtcataga tctgaatcgt cttgaaggtg atatcgtcac tttcaacaaa ctgattgaag
      481 ttcacatgtt cttgaatgag ctcatccgga ttgttttgtt tattatagaa agtgatcact
      541 ttgttcttct tctgaagaag ataaaagtcc accatgcgaa attcccgcgc ttgcttcagg
      601 cgttcggtca gagaatcaag attgtcttta ataatgagat tgccggagaa ttctaaaaca
      661 aaatcgacgt cttttttgcg attttgaatg acctgatcca ctcgtgacgc aatattaaaa
      721 taatattctg ctgacacgat gccaacgtaa gctatccaga acgctgcgat agcgaatagc
      781 agaatatttt ttctattttt ccgtctcact gacaagtcat tttctccata gctaaaacca
      841 aaacgaacca acttcaattc atttactatg agccgtatcg gcctaagacg gaaaaactgt
      901 actggactgg actattgaat tcttaaatca actttcgtcc aggcgcctgt gcttaatata
      961 atgacttttt ataccttttc cagaactccg gaaaagtatg atatttaagt tatatctatc
     1021 tatggcgaaa ttttccatcc gtatcaaaga aatattcata aatatattta tatttatatt
     1081 tatatttatt ttatatttac aacagaaatg cttatcaaaa acgtctacaa ctgcgagatt
     1141 ttcgtattaa aaatttaaga aattaggtta agtgttttcc tatttattcc gttatttaag
     1201 acatgaaatt aatcactaat caccggggta caagcctggt cactgttctt atggcagttg
     1261 gcattggcgc cattgtttta gctgggatca cgaccatgat cacagatgtg ttcaagtccc
     1321 agcgcacggc tcagtctaaa gaggcccacc gggaactgac cgccaatatc cgtcaacttt
     1381 taacagatcc ggctatttgc tcagtcagct ttaaaggaag taacccgtca gggaagggat
     1441 atactaaaac tcaaatcgtg gatgccgcca ctccttctaa tgttaaatat caaaccggag
     1501 tggattacct taatcatctg gtcaccatta cggggtttga ggtcaggggt tttgtggccg
     1561 acaatccccc tgttaacgat aaaataggaa aggctgagct caatattaaa atgaataagc
     1621 ttggctccac cattggcagt ccacaaatga cgacgacggt ttttttgcaa gcgactctgg
     1681 atgcttccta taatctcaat agttgttatg ccctgggact tgcggattca ctgtggcaaa
     1741 tctctcccgc gaatatggcg gatatttatt atcccagcgg caa
//
LOCUS       DATBTE010000402         3789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_467123, whole genome shotgun sequence.
ACCESSION   DATBTE010000402 DATBTE010000000
VERSION     DATBTE010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467123"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VIG33_15470"
     CDS             <1..878
                     /locus_tag="VIG33_15470"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY8272290.1"
                     /translation="SSRIIKELLRKKIGFTGLVITDDIQMKGLAEISNPYEAGLRSLK
                     AGADIVMMTWSFKDQAKAIEHVKQAVLNGDFPMVELNEKVSRILAVKKFLQSKHSLQG
                     AIGSSKIVALSTKKLREIESRILDFNIKMATRRSQETGDRVPAAVLEKAINIKTPICI
                     FSSTNEFINSFKKSNLPTSFTRTTASTKPQALIDSIIKNDCSYWLYTIYGPKTAAILK
                     TLPRAVAKKILIINLSAPSLITDSTKFLNVVNLYFPHPEAGKKVAENLQALASPELTD
                     PLPAKPDDIQTGQEL"
     gene            complement(952..1362)
                     /gene="atpC"
                     /locus_tag="VIG33_15475"
     CDS             complement(952..1362)
                     /gene="atpC"
                     /locus_tag="VIG33_15475"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEY8272291.1"
                     /translation="MFKLTLVTPEKRIVVDQEIDEVTVPAFKGELNILPGHAPLITTL
                     ETGILKYKLKGQSEQKVAISWGYCQVSPLGVNILAEYAAVPMDLQLEDLKKALTAAEL
                     KLSSESLNEEQWAEAQREVAKARTGMEIIPGTWS"
     gene            complement(1372..2778)
                     /gene="atpD"
                     /locus_tag="VIG33_15480"
     CDS             complement(1372..2778)
                     /gene="atpD"
                     /locus_tag="VIG33_15480"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY8272292.1"
                     /translation="MANGKIKQVMGPVVDVEFEGGELPPIFTALRSTNKSISDAEMNI
                     VFEVAQHLGDGVVRTISMDSTEGLVRGEKVTSTGNMILAPVGPEVLGRIINVIGEPVD
                     EQGPVKGKDNWPIHRAAPKFEDLAVAAEMLMTGIKVVDLLAPYAKGGKIGLFGGAGVG
                     KTVLIQELIRNIATEHGGYSVFAGVGERTREGNDLWAEMKQSGVISKTALVFGQMNEP
                     PGARARVALTGLTVAEYFRDVEKQDVLFFVDNIFRFTQAGAEVSALLGRIPSAVGYQP
                     TLATEMGAMQERITSTKSGSITSVQAVYVPADDYTDPAPATTFTHLDATTNLDRDIAA
                     MAIFPAVSPLTSTSRILDPQVVGEEHYKTARDVQSILQRYKELQDIIAILGMDELSED
                     DKLVVSRARKIQRFLSQPFFVAEQFTGLSGKYVDVKDTVKGFREILDGKHDSLPEQAF
                     YMVGTIEEVVEKAKKLQA"
     gene            complement(2794..3675)
                     /gene="atpG"
                     /locus_tag="VIG33_15485"
     CDS             complement(2794..3675)
                     /gene="atpG"
                     /locus_tag="VIG33_15485"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEY8272293.1"
                     /translation="MASLKDIRARIESTKNTQQITKAMKLVSAAKLRKAQNNITNMRP
                     YALTLRKVIADIAITNKITHPLMEQKSEVKKVLLVVLTSDRGLCGGFNSNINKFAEIY
                     YKNNIKTCEKIEFLFVGRKGSEYFTKKGYTAVDSITRLDKDISYDLASHVADRLMKQY
                     LSGDYDEIRLIYNEFKSAISQQVVCETLLPIDIQNTSFDTTGTAVGFSVDMIFEPAPQ
                     QIIEQVLVKHFDLQVYRTMSESVAAEHGARMTAMENATNNAKEMINSYTLTYNKLRQE
                     KITTELTEIVSGAEALK"
BASE COUNT         1095 a          881 c          719 g         1094 t
ORIGIN      
        1 tatcctctcg cattatcaaa gagcttctca gaaaaaaaat cggcttcact gggttagtta
       61 taacggatga tattcaaatg aaaggactag ctgagattag caatccctat gaggcgggtt
      121 taagatccct aaaagctgga gcagatatag tcatgatgac atggtcattc aaagatcaag
      181 caaaagcaat tgagcatgta aagcaggccg ttcttaacgg cgattttcca atggttgagc
      241 tcaatgaaaa ggtttctcgc attttggctg ttaagaaatt cctgcagagc aaacactctt
      301 tgcaaggggc cattggaagc tctaagatcg tcgctctttc tactaagaag ttgcgagaaa
      361 tcgaatcacg tattctcgat tttaacatca agatggctac gcgaagatct caggaaaccg
      421 gagatcgcgt cccggccgcc gtgcttgaaa aagcaatcaa tattaagact ccaatttgca
      481 tattttcttc aactaacgag tttattaatt cctttaaaaa atcaaattta ccaactagct
      541 ttacaaggac gactgctagc actaaaccgc aggcactaat cgactcaata atcaaaaacg
      601 attgtagcta ttggctttac acaatttatg gaccaaaaac tgctgcaatc ttaaagactc
      661 ttccgagggc tgtggcaaaa aaaattctga ttattaatct cagcgctcct agcctgatta
      721 cagactcgac aaaattttta aatgtcgtga atttgtactt tccacatccg gaggccggaa
      781 aaaaagtcgc tgaaaatctt caagcactag cgagcccaga gctcaccgac cctttgcctg
      841 caaagcctga tgatattcag acggggcaag agctttaatt cttcaaggca aaataaaaaa
      901 agccctctct tagggaaggg ctttctcata tttttaaatt ttctttaact cttaagacca
      961 agtgcctgga atgatttcca tgccagttct agcttttgcc acttctcttt gagcctcagc
     1021 ccattgttct tcattcaaag actcgctgct aagcttaagt tctgctgcag tcaaagcctt
     1081 tttaagatcc tcgagttgaa gatccattgg aactgctgca tactcagcca aaatgtttac
     1141 tcctagcgga ctcacttggc aatagcccca gctaatagca actttttgtt ctgactggcc
     1201 tttgagtttg tatttcaaaa ttccagtttc caaagttgtt atcaaaggag cgtgaccagg
     1261 gagaatgttg agttcccctt tgaaagccgg aaccgtgact tcatcaattt cttgatcaac
     1321 tacgattctt ttttccggtg tcacgagtgt tagtttaaac ataagttatc cttaagcttg
     1381 aagcttctta gctttttcaa caacttcttc gatagttccg accatgtaga atgcctgttc
     1441 aggcaagctg tcgtgtttac catccaagat ttcgcggaag cctttaacag tatctttaac
     1501 atcaacgtat ttaccgctaa gacctgtaaa ttgctcagcc acgaagaacg gctgggacaa
     1561 gaaacgttga atcttacgag cacgtgatac caccaattta tcgtcttcag acaattcatc
     1621 catacccaga atcgcgatga tgtcttgcaa ctctttgtaa cgttgtagaa tcgattgaac
     1681 atcacgagcc gttttgtaat gctcctcgcc caccacctgt ggatctaaaa tacgagaagt
     1741 tgaagttaat gggctcactg cagggaagat cgccattgcc gcgatatcac gatccaaatt
     1801 ggttgtagca tccaagtgag taaatgttgt tgcaggagct ggatctgtgt agtcatccgc
     1861 tggaacgtaa accgcttgaa cggaagtgat tgatccggat ttagtagaag taatacgttc
     1921 ttgcatcgcg cccatctcag ttgcaagggt cggctggtaa cccaccgctg aaggaatacg
     1981 acccaacaac gcagaaacct cagcgcctgc ttgagtgaaa cggaagatgt tatcaacgaa
     2041 gaaaagaacg tcctgttttt ccacgtcacg gaagtattcc gcaaccgtta aaccagtcaa
     2101 agcaacacgg gcacgagctc caggaggttc attcatttga ccgaaaacaa gcgccgtctt
     2161 gctaataacg ccagattgtt tcatctccgc ccacaagtca ttaccttcac gagtacgctc
     2221 acccaccccg gcaaatacgg aataaccacc atgctcagtc gcaatgttac gaatcaactc
     2281 ttggattaat acggttttac caactccggc acccccgaag aggccaattt taccaccttt
     2341 tgcataagga gccaaaagat ccacaacttt gataccagtc atcaacattt ccgccgcaac
     2401 cgctagatct tcaaacttag gagctgccct atggattggc cagttatctt tacccttcac
     2461 tggtccctgc tcatccacag gctcaccaat aacgttgata atacgaccta aaacttcagg
     2521 gcctactgga gcaagaatca tattgccagt gctagtaact ttttcacctc tcacaagacc
     2581 ctctgtagaa tccatggaga tcgtgcgaac cacaccatca ccaagatgtt gcgctacttc
     2641 aaatacgatg ttcatttctg catcagagat tgatttattt gtagaacgaa gagctgtgaa
     2701 gatgggagga agctcgccac cttcgaattc aacgtcgaca actggtccca tcacctgttt
     2761 gattttaccg ttagccattt tcatacgctc ctattatttc agtgcttctg caccactcac
     2821 gatttctgtt aattctgtag taattttttc ttgtctcaat ttgttatatg tcagcgtgta
     2881 agagttaatc atctctttcg cgttattggt cgcattttcc atagcggtca tacgagcacc
     2941 atgttccgct gccacgcttt ccgacattgt tctgtatact tgaagatcaa aatgtttcac
     3001 cagcacttgt tctataattt gctggggagc cggttcaaaa atcatatcta cagaaaagcc
     3061 cactgcagtt cccgttgtgt caaaagaagt attttgaata tcaattggaa gcagagtttc
     3121 acaaacaacc tgctgagaga tcgcagactt aaactcattg taaatcaaac gaatttcatc
     3181 atagtcacca gagagatatt gcttcattaa gcgatccgcc acatgtgaag ccaagtcata
     3241 agaaatatcc ttgtccaggc gagtgatgga atctacggct gtatagcctt ttttagtgaa
     3301 gtattcactt cctttgcgac caacaaaaag aaattctatt ttttcgcaag tcttaatatt
     3361 gttcttataa tagatctctg caaatttatt gatattgcta ttaaaaccac cgcaaagacc
     3421 acgatccgaa gtcaatacaa ccaaaagtac ttttttgact tctgattttt gctccatcaa
     3481 aggatgagtt atcttgttag taatagcgat atcagcaatc acttttcgca aagtaagcgc
     3541 gtaaggacgc atattcgtaa tattattttg cgccttacgt aacttcgcag cagaaacaag
     3601 tttcattgcc tttgtaatct gctgtgtatt tttagtggac tcaatccgag ccctgatatc
     3661 ttttaaactt gccatttcgg caccctatta attcgtagaa ggttggaaaa ccactttaaa
     3721 ctcttcaaga gcagcaacaa gcgtcttctt gatatcatct gccatagctt tcttttcagc
     3781 gatattttt
//
LOCUS       DATBTE010000403         1557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1763026, whole genome shotgun sequence.
ACCESSION   DATBTE010000403 DATBTE010000000
VERSION     DATBTE010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763026"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..>1557
                     /locus_tag="VIG33_15490"
     CDS             297..>1557
                     /locus_tag="VIG33_15490"
                     /inference="COORDINATES: protein motif:HMM:NF037255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN-like domain-containing protein"
                     /protein_id="HEY8272294.1"
                     /translation="MKDLFPGHFKEVEHQLRDIWDTSLFVFDANILLNLYRYSDTTRQ
                     EFLRILEKIKDRVWLSHRAAEEYLNNRLSVIDQQEKSYDDMTKAIEILKRDLENARQH
                     PFVSQNIMTKVSSVFDELCSELKENKSVHTARIYNDEIKDAVASIFKNRVGLPYERSR
                     LEELMSEGEERYMQKIPPGFKDGSKSGDSEVFTEKCRKYGDLIVWTQVIDKAIESGKG
                     VVLVTDDKKEDWWEKFKGKTIGPHPELVREFKDRANNTFYMYQADRFLELARENLGEQ
                     VSDEIVAEIREVRHLEKLAQKRQKEKEYVQGQKHERRQFLREIEYGKHRLATYYEEQN
                     KLQQRMHELESIQNGMYQHQDDLVREEGYESAEFKHFMNRFIEISEEFALVRKKLEHS
                     SSKFKETQMHVFELEKELASRMKISEQE"
BASE COUNT          533 a          248 c          362 g          414 t
ORIGIN      
        1 gtgcttgcac gtaccgatta cgagcggttg acccggtatt gatgcttaaa tagagtaggg
       61 ggcgctatgt gtactttgct ggcttggcgg tatggtcgag ctaaacggta cgcacagcgt
      121 actctacggc gctatcgacc caaatcggcc tttccttatc tttgtttgac tggcagatta
      181 gtgccgcatg attgacgcta gggaaacagg tatgtaggat gtgtttcggt atcatcaact
      241 tgctaaacag tacgttcagc ggaacattac gcaaccatag gttagttaga ggtagtatga
      301 aagacttatt ccccggccat tttaaagaag ttgaacacca gcttagggac atctgggata
      361 cgtcattatt cgtatttgat gcaaatattc ttcttaatct gtacagatat tcagatacaa
      421 ctcgccaaga gtttctacgg attttagaaa aaatcaaaga tagggtatgg ctttcccata
      481 gagctgccga ggaatatttg aacaaccgat tatcggtaat tgatcagcaa gagaagtcat
      541 atgacgacat gaccaaggct attgagattt tgaaacgtga cttggagaat gccagacagc
      601 atccttttgt tagtcaaaat attatgacta aggtttccag tgtttttgat gagctttgtt
      661 cagagcttaa agaaaacaaa tcagttcata cagcacgtat ttacaatgac gaaattaagg
      721 atgctgttgc ctctatcttt aaaaatagag ttggtctccc ttatgaaaga tcacgtttag
      781 aagaactaat gtctgagggt gaagaaaggt acatgcaaaa aataccacca gggtttaagg
      841 atggctcaaa gtcaggagac agtgaagttt ttactgagaa gtgcagaaaa tatggagatc
      901 ttatcgtgtg gactcaggtt atcgataaag caattgaatc tgggaaaggt gttgttttag
      961 ttactgatga caaaaaggaa gactggtggg aaaagtttaa agggaagact attggacctc
     1021 atccagagct agtgagagag tttaaggatc gagcaaataa tacattttat atgtatcaag
     1081 cagaccgatt tctagagtta gctagagaaa atctaggtga gcaggttagc gacgaaatcg
     1141 ttgcagaaat tagggaagtc agacacttgg aaaaattagc tcaaaaacgc caaaaagaaa
     1201 aggagtatgt tcaagggcaa aaacacgaaa gaagacaatt tcttagagaa attgaatatg
     1261 gcaaacatcg attggcgact tattatgaag agcaaaataa gttacaacaa cgcatgcatg
     1321 agcttgaatc aatccaaaat ggaatgtatc agcatcaaga tgatctggtt agagaagagg
     1381 gttatgaaag tgcagagttt aaacatttca tgaatcgctt tatagaaata tcagaagaat
     1441 tcgcgctagt tagaaaaaaa cttgaacatt catcctcaaa attcaaagaa acgcaaatgc
     1501 atgtgtttga attagaaaag gaattggcca gtcgcatgaa aatcagtgaa caagaaa
//
LOCUS       DATBTE010000404         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_56754, whole genome shotgun sequence.
ACCESSION   DATBTE010000404 DATBTE010000000
VERSION     DATBTE010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56754"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(570..854)
                     /locus_tag="VIG33_15495"
     CDS             complement(570..854)
                     /locus_tag="VIG33_15495"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY8272295.1"
                     /translation="MRILWTKKAEKSLDKIFQHTVDNFSFELAERTFVEIKLAVSKLS
                     DFPYLGRRIGGHEEKRQLVISGNAIIYEIVLGASPYIIIRNVRPRGTNQV"
     gene            complement(1176..>1694)
                     /locus_tag="VIG33_15500"
     CDS             complement(1176..>1694)
                     /locus_tag="VIG33_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8272296.1"
                     /translation="QNVDYEYQENLKPPSTAEISCRFAPLTAEQTQRVYDYCSTGLLE
                     RYNSMDPPGEPYFNKPRIWRLSVYDHAGQVRHFCYGINSGLIEPAEEEVENISWTTEV
                     PIAKLFAALEAGESLTSMYLRINDMVFPPNIEEGIQSADIVEDPLIRCLFNGVFGAYQ
                     IAQLKRLKFAVF"
BASE COUNT          468 a          382 c          351 g          493 t
ORIGIN      
        1 ccaaggaaga agaacgggac actgaaagtg tcgcggttcg attcctgtag tttcaaaatt
       61 tctgcaggca cccagcccgt agaaccagcg gatctcctgc ggggttcgat cccggaagga
      121 acccagggaa tgtatttctg caggctcctt tataattgcc tttccctatt ttccaaattc
      181 tttggattac agaatggctc cgagtttcgg ataaagtgcc gttacacgga ttgaaaatct
      241 tgagggcata tttcttcaac gtggccttga agctcgttca agagttgttc ttaaaaaagt
      301 tattgatatt tcgaatcaaa aaattcaaaa taatcgtgcc tgcaaaagaa tacctcaaac
      361 aggacgaagc cttcacaact acgtaccatt ttttttgacc ttcaaagctc caatggtagc
      421 agagaagcaa actcaaaatg aagatctagt ttatctacaa attcatctcg atatttttgg
      481 cagaataaaa atctccttct tgttctttaa gtcgtttcag ttaaatacat cgaggctaac
      541 ggctccccgc ttctaaggcg gcagagtcgt tagacctgat tcgttcctcg agggcgaacg
      601 tttctgataa taatataggg agaggcaccc agaacgatct cataaataat ggcgttgcct
      661 gagatgacca gttgccgctt ttcttcatga ccgccaatcc tgcgacctaa atatgggaag
      721 tcggacaact ttgacactgc tagcttaatt tcgacaaagg tccgctctgc caattcaaaa
      781 gaaaagttat caacggtatg ttgaaatatc ttatcgagac ttttttcggc tttttttgtc
      841 catagtattc gcatttgaaa atccatattt cttgccaagc cgagcgaaca cttcatcact
      901 ttcgataaga tgggtatgat caataggctt ttcaaactct ttcaaaagtt caaggtcatc
      961 tattaagcaa tcatattctt gttttgagat aagaacgacg tctcctgatt ttgttggaac
     1021 aatataacga tcgcccttac tgacacgatc aagcgtgttg tacaggtctt ctcttaattc
     1081 ggatggccta gtaatctttg gtgaccccat cgcccgagcg taccaaaatg cgtacgtttt
     1141 gtcaagaggt tttccaggtt ttgactgtcg cgggttcaaa agactgcaaa cttcagtcgc
     1201 ttcagttgcg cgatttgata agcaccgaag acgccgttaa aaagacaacg tatcaatggg
     1261 tcctcgacaa tatctgcgga ctgaattcct tcttcaatat tgggagggaa aaccatatca
     1321 ttgatgcgca aatacatcga ggttaacgac tcccccgctt ctaaggcggc aaagagttta
     1381 gcaatgggta cctcagtggt ccaagatata ttttctacct cttcctccgc aggctcaata
     1441 agtccgctgt ttattccgta acagaaatgt cgaacctgcc ccgcgtgatc atagacagaa
     1501 agtcgccata ttcttggctt attaaagtac ggctctcctg ggggatccat tgaattatat
     1561 ctttcgagaa gacctgtgct gcaatagtcg taaactcttt gcgtttgctc cgccgtcagc
     1621 ggcgcgaatc gacaagagat ttccgcagtt gatggcggct ttaaattctc ctggtattca
     1681 taatcaacat tctg
//
LOCUS       DATBTE010000405         1842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1627658, whole genome shotgun sequence.
ACCESSION   DATBTE010000405 DATBTE010000000
VERSION     DATBTE010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627658"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VIG33_15505"
     CDS             <1..301
                     /locus_tag="VIG33_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272297.1"
                     /translation="LKVNSAVLLHKFGEARVRKFLGKSFFLQEYSPFRRYYMMRNRII
                     LYKRYGMFKGAWFWLDAKNAIKDLIKLTFFESKKRAKFIAVIKGIVDGLCWRESK"
     gene            508..>1842
                     /locus_tag="VIG33_15510"
     CDS             508..>1842
                     /locus_tag="VIG33_15510"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8272298.1"
                     /translation="MIIGRKQEIDELNSDYLSEKSSLIVIYGRRRIGKSALVANYASK
                     KPFLSFEALEGVNKAKQIEHFISQLNLQIKDEFLSQMRFGRWDEVFRYLTEKLKQGKE
                     KQVLFFDEFQWMASGQSSLVSLLKFFWDTEWKKLNVQLILCGSIASFMVDKVIKSKAL
                     YGRVDLERVITELRPFELRKFLDKSRSLHEVLKYILVMGGVPKYFELINKNESFEKNI
                     QRLAFNSSGYLFNDYEKIFYAQFKEATTYERIVRFILKKPQDLEDISRHLKMQSSGGV
                     KRYLKNLEAARFIRSYKPIQKANTNVVKYKMLDEYLIFYHKYIQPHHRQIQNAVPNNI
                     FVTKVKSEWTPWLGIAFENFCLKYNAVICEVLGIRDLVEEVGPYFERKDKAGFQIDLL
                     IKRSDHTWVICECKYYEGLVGVEVTTEVEKKINRLEIPRGISVEKVLITVSGA"
BASE COUNT          586 a          282 c          410 g          564 t
ORIGIN      
        1 tttaaaagtg aattcagcgg ttttactgca taaatttggt gaagctcggg taagaaaatt
       61 tttaggaaag tctttttttc tgcaggagta ctctcctttt cgtcgttact atatgatgcg
      121 aaatcggatc attctttaca agcgttatgg aatgtttaaa ggggcctggt tctggttgga
      181 cgcgaagaat gcgataaaag atcttattaa gctgactttt tttgaaagca aaaaaagagc
      241 taagttcatt gcggtgatca agggtattgt cgacggtctc tgttggaggg aatcgaagta
      301 actcttccca agttttttgg agtcttattc tgttcgtttt aaccaagctg ccctggtcag
      361 tttatgagtt caaaaaaata gcttggaatg gagtaactaa gttttccgta catagagatg
      421 aaaaaactct atgtacggaa aaatggtaaa taatagcttg aatccgtaca tagagttata
      481 taaactctat gtacggaagt gctaactatg attattggca gaaaacaaga gatagacgag
      541 ctaaatagtg actacctcag tgaaaaatcc agtttaattg tcatttatgg gcgtcgaaga
      601 attggaaaaa gcgccctggt cgcaaactac gccagtaaaa aacccttctt gtcttttgag
      661 gctcttgagg gagtcaataa ggcaaagcag attgaacatt ttatttcaca gctaaatctt
      721 cagattaaag atgagttctt gtctcaaatg agattcggga gatgggacga agtctttagg
      781 tatttaactg aaaaattaaa gcaaggtaaa gaaaagcaag ttctgttttt tgatgagttt
      841 caatggatgg cctccggcca atcaagctta gtctcattat taaaattctt ttgggacaca
      901 gagtggaaga aactcaacgt tcagcttatt ctttgcggat caatcgctag ttttatggtt
      961 gataaagtga tcaagtcaaa agcactttat ggtcgtgtgg atcttgaaag agtgattacg
     1021 gaattgagac cttttgaact tcggaagttt ctagataaaa gcaggtctct gcacgaagtg
     1081 cttaaatata ttttggtcat gggtggcgtg cctaagtatt ttgagcttat taataaaaat
     1141 gagtcctttg aaaaaaatat tcagcgctta gcctttaata gttcaggcta tctttttaac
     1201 gactatgaaa aaatatttta tgcacaattt aaagaagcca cgacttatga aagaattgtc
     1261 cgctttatat taaaaaagcc tcaggacctg gaggacattt cgcgtcactt aaaaatgcaa
     1321 tcgagtggag gtgttaaaag gtatttgaaa aatttggaag ccgcacgttt tattcgttcc
     1381 tacaagccta ttcaaaaggc aaataccaat gtggttaagt ataaaatgct cgacgaatat
     1441 ttgatctttt atcataaata tattcagcct catcataggc agattcaaaa tgccgtacca
     1501 aataatattt ttgttaccaa agttaagtct gagtggacgc catggctggg aatagccttt
     1561 gaaaactttt gtctcaagta caacgccgtt atttgcgagg ttttggggat tagggacctt
     1621 gtcgaagagg ttggccccta tttcgaaaga aaagacaagg ctggctttca aattgattta
     1681 ttaattaagc gcagcgatca cacttgggtc atatgtgaat gtaaatacta tgaaggactt
     1741 gttggcgtgg aagtgacgac ggaagttgaa aaaaaaataa ataggttgga aattccgcgg
     1801 ggaatttctg tggaaaaagt actgattacg gtgagcggag ct
//
LOCUS       DATBTE010000406         8892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1627733, whole genome shotgun sequence.
ACCESSION   DATBTE010000406 DATBTE010000000
VERSION     DATBTE010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 8892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8892
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627733"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VIG33_15515"
     CDS             <1..374
                     /locus_tag="VIG33_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272299.1"
                     /translation="QTLIEEWRNPNVHSYGTDIAKFASDLVEVFTAVQKFLHSSEIAV
                     VEPIENSSSGAFSPEGPAEIEESFESQRLQSYIDSAEGGKVADECQNASVWQTDQAEL
                     CQSDDALECDRSYARQCKKLQ"
     gene            414..1397
                     /locus_tag="VIG33_15520"
     CDS             414..1397
                     /locus_tag="VIG33_15520"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEY8272300.1"
                     /translation="MAENIETSFSKLMGTQTPIIAAPMFLVSNEDLVVAASEAGGIGT
                     VPSLNYRPAEKFKAALENIKKRTKKPIGVNIIVNKSNTRQEGDLKIALDAGVELFITS
                     LGNPKTVIQEAHKNGAKVICDVTNLEFAQKVQDLGADGVIAVGAGAGGHAGPISPLVL
                     IPWLKTQLQIPIIAAGGISHGAQIAACLALGASGVSVGTRFIASKEAGVDMAYKQAVV
                     SATPEDIVMTARVSGTPAAVINTEYVKKLGTDLPWLIKSLKDNPMTKKYIVPLIHLMG
                     MKALEKSATNPSWKTVWSAGQSVGLVEDILSTAEIYHKLISEYQAAKANLP"
     gene            complement(1394..1888)
                     /locus_tag="VIG33_15525"
     CDS             complement(1394..1888)
                     /locus_tag="VIG33_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HEY8272301.1"
                     /translation="MSKQPLSLFKTNLILKAYGLFKIPLLAFVSPKCLELSPTRTVIR
                     IKLGRRSQNHLKVMYFGALSIGAELAVGLTAVTSIYESGQKIDFIFKDFKADFLKRCD
                     GDVYFICDEADKVRELIEKAKTTSERLESCFKVYSTVPSKSKTDKVATYELTLSVKNR
                     SKGL"
     gene            complement(1922..2590)
                     /locus_tag="VIG33_15530"
     CDS             complement(1922..2590)
                     /locus_tag="VIG33_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272302.1"
                     /translation="MKNKTNSKKMLASFLIGGSLLIGLQAIAETTAKEGLEKIKSNVS
                     NSKANLGDYQKNLKSVEKNIGEIQKAKAQVDGQRKTVVSQHEENKKFLLKVEAQEKEL
                     HTLMAEEKNKKALEEKKLQDLQTAMNKLQENIAKRDANLTDYETQMNSIQEEKKIWVS
                     RGEQIKQQGALVDQRNRALATSENDWKNKKKGYEGEVGRWSKEVDRQQKLQDNYTSLT
                     EVRD"
     gene            complement(2623..4698)
                     /locus_tag="VIG33_15535"
     CDS             complement(2623..4698)
                     /locus_tag="VIG33_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272303.1"
                     /translation="MKKHKLVLALMTAGLAAGTAHAQKANMYMKSSNVPAYKLVQNKK
                     KVTEIPRLNIGAEREVQANVKPLQIPEAGKLSLNPIVKRPSPAEVRIPLELTKFPPSN
                     GSRVVTGKNAIANVPELKLLNEVADPLLDMVEAKPELMPLQEMKPNDYKMLQALIFLG
                     NQKNYELALGLFSELIEDPEYRTEASYNYALTAKGLGINSEFRMGMIKVASDAKSKEW
                     QEKAVRQLVENISALKVSDIKYIDPLITKYDIDTTQNEAYQLSRAKYYLEAGQLELVE
                     DSLMFIGEKSPKYPEATMISALSLYRQGKVDEAIMTLNKLLELTSDDKKGLIRNVGAL
                     TLGRMYFQKGAYKDSFQAYLKVEKSNPMWLQAMTEQAWTQIMAEDYEGAAGNMFSLHT
                     DFFKNAFAPESYTVRTVGYLNLCQYGDGIQILNEMKKKFAPLKGKLASYQSANDKNPA
                     HYYETIKAWMKNIERKEVDGLPRPFIVEMARHPSFTVPQTEINTTEDEISRFNKVTLT
                     LLEREKMMLKKSVEAGKEISEIRKKLIDNKKSASLQESLAKAERRQMSFRIQQFIAQK
                     ARTSMKKLREMGIARMEKEKSELREQAGLALKRRMTQLAGNLNRVLEQNEVLSYELYS
                     GAGEHIRYQMAGGNVTEKERPELKVEADKSLNWKFQGEIWEDEVGHYRSSLKNVCPKE
                     DKVTSLDEN"
     gene            complement(4726..5478)
                     /locus_tag="VIG33_15540"
     CDS             complement(4726..5478)
                     /locus_tag="VIG33_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HEY8272304.1"
                     /translation="MKTLTLTLMLLGSSLAYAQTSPATTGGNDKLDIRKLEQKYWSAK
                     DDDFAVVQNRRYTKANRFFLSANYGVPFNDPFSSGNIYGMTFGYFFNERWGLDLDYKN
                     ASLHDNDATSQFINQYGTYPDHNVFKSSQILSFTLVPFYAKMSFMDNTIIYFDMGFSV
                     GAGTLKYAIQKVEGNEEVSSAVGKLSVFQQIFFSEHFALRADLSNTWASQKRLKYYLP
                     NSGGSHNYTTERDLGAKMTNDTSMLLGVTYWF"
     gene            complement(5497..6978)
                     /locus_tag="VIG33_15545"
     CDS             complement(5497..6978)
                     /locus_tag="VIG33_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEY8272305.1"
                     /translation="MNNILIVKQRLKNGTTKTWKLHSTQPVYTFGGSRHADVISISPT
                     SQGMQGTFECRDGKWWYIDMSLNNVMSSSDCQICLEAEKTLEIADSTLNITPLLRESD
                     LYQKLERNPLSFEKDKKPYQLFLVKLHEKVIETRVLPMGQNFIPEMAITKKVIPAKDT
                     DSWIQYTLDQYIVRQKTVYLTDEEAMNRVTASQIVDKDTKKGFLILLLAAFMIGGLSL
                     MAPKQEQMATVVIPPPAQRVVVKTDHKKKVKVAEAVKPAPPQPVPQPSAPAKEPQKNS
                     AQNSAPKGAGKIANMLKAVADPRISRLIGKVSAQAAKSGNVIVSNGIKAGSGASGRAL
                     AAVGNIDRSGRDYGTDSTAQGVMISTAGRGGGKSTSGIGGLGAGNTGNGGVGLIEEES
                     EVVGGLDREVIAQYIKSQLGQILYCYERQLSANPQLFGKVAVKFTIAGTGQVETQAIG
                     DTTLKNATVEGCILNKVAKWKFPAPAGGTKVLVTYPFLFKSTN"
     gene            complement(7038..7280)
                     /locus_tag="VIG33_15550"
     CDS             complement(7038..7280)
                     /locus_tag="VIG33_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272306.1"
                     /translation="MKQLILGLIVVLGFVTNAVAKSKVTRKVQEVNFGEMNLKGTIRN
                     PDGAYLVQKKGIKFLPLHDIQKDMDGRIRESSLYLR"
     gene            complement(7363..>8892)
                     /locus_tag="VIG33_15555"
     CDS             complement(7363..>8892)
                     /locus_tag="VIG33_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adventurous gliding motility protein U"
                     /protein_id="HEY8272307.1"
                     /translation="EIYTKLMQGMDNKNQARMITKIMATYGNDTNHPDYQKLQEKTLA
                     INMEPYATQILTERAEKLLISGKQTPAFDAAKKIMARNIPSEEKAPARLIQAQVLEAE
                     LIQQSVKAKMDKFAVVLGLKTEKLDKAQTAYLNAMTLSKDEKVVRKALAGLDRCYTNY
                     IDSLQNMPMPGGLTPDEKETLKGELAKITTPIKGKKAENEQRLKTLMVVRATSDGKGR
                     DYSELNPEASISPTVSYPPVSQFEGYLTAKTDMSPVRVQKGQSKVCNRSFTFKEASIQ
                     SILDNANACYISKQYDVTEKLSLELAKYKDTRTLGLYYLSLAADAREQKEKALWLVDE
                     LMKTQSENALVLYQKGRLMYKIEDINAAIPFFAKVVDMNIGSSEIATFAAVKAFTEKD
                     YISSTENFSRLTQDQVYNYNMGPLFSEAWALRGEGEKGLQFITELSAKKKDNVDILLQ
                     QAHLIEVYKSSDVKTAQQVYERIARITTKADLKEWIGKKLALLKGQGSTEGKVTSLES
                     N"
BASE COUNT         2131 a         1949 c         2033 g         2779 t
ORIGIN      
        1 cgcaaacgct tattgaagaa tggcgaaatc caaatgttca ctcttacggg acagatattg
       61 cgaaattcgc ttcagacctc gtggaagtat ttaccgctgt tcaaaagttt cttcattctt
      121 cagagattgc ggttgtagag ccaatagaaa attcttcttc tggtgccttc tctcctgaag
      181 gtcctgcgga aattgaagaa tctttcgaga gccagcgcct ccaatcctat atcgactccg
      241 ccgaaggagg aaaagttgcg gacgaatgtc agaatgcctc cgtctggcag actgaccaag
      301 cggagctctg ccaaagtgat gatgctttgg agtgtgaccg cagctacgcg cgtcagtgta
      361 aaaagcttca ataagatcca aaaatatttt aaagtgatcc ttttagaggg cttatggcag
      421 aaaatattga aacttctttt tctaaattga tgggaacgca aacccccatt atcgccgcac
      481 ctatgttcct ggtgagcaac gaagacctgg tcgttgctgc gagtgaggcg ggaggcatcg
      541 gaacggttcc ttctttgaat tatcgtcctg cggaaaaatt caaagcggcc ctcgaaaata
      601 ttaaaaagcg cactaagaaa cctattgggg tcaatattat tgtgaataaa agcaataccc
      661 gtcaggaagg ggatttaaaa atcgcccttg atgccggagt ggaattattc attacctcct
      721 tgggcaatcc taaaactgtg attcaagagg cccataaaaa tggggcgaaa gttatttgtg
      781 acgtgaccaa cctagagttc gcgcaaaaag ttcaggatct gggtgctgac ggggttattg
      841 ccgtgggagc tggggccggt ggacatgcag gtccgatctc tccgctggtt ttgattcctt
      901 ggcttaaaac tcaattgcaa attcctatta tcgcagctgg tggtatttcc catggagcac
      961 agattgcggc ctgcctggcg ttaggcgctt ctggtgtgag cgtgggaact cgttttatcg
     1021 cctcgaagga agcaggagtg gacatggctt ataagcaggc tgtggtttcg gcgacacctg
     1081 aggatattgt gatgaccgcg cgggtgtcag gaactccggc ggccgttatt aatacggaat
     1141 atgttaaaaa gttaggaaca gatcttccgt ggttgatcaa gagtctcaaa gataatccaa
     1201 tgactaaaaa atatatagtg cctttgattc atcttatggg catgaaagcc ttggaaaaat
     1261 cagcgaccaa cccttcctgg aagaccgttt ggagtgcggg acaatcagta gggctggtag
     1321 aggatatttt atccactgcg gaaatttatc acaagttgat ctctgaatat caagccgcta
     1381 aggcgaattt accttaaagg cccttcgagc gattttttac cgagagggtg agttcataag
     1441 tagccacttt atcggttttg ctcttggaag gaacagtaga gtagacttta aaacagctct
     1501 ccagtctttc gctggtggtt tttgcttttt ctatgagttc gcgaaccttg tctgcttcat
     1561 cgcaaatgaa ataaacatca ccatcacagc gctttaaaaa atcggcttta aaatccttaa
     1621 agataaaatc gattttttga ccgctttcat aaatggaagt caccgccgta aggcccaccg
     1681 cgagttcggc gccaatgctc aaggctccga agtacatcac cttaaggtga ttctgactgc
     1741 gacgtccgag cttgattcta atcaccgtgc gagtggggga gagctcaagg cactttggac
     1801 tcacgaaagc caagagaggg attttaaaaa ggccataagc ttttaatata agatttgttt
     1861 taaataaact tagcggttgc ttggacataa aaaaagctct ccctgaagga gagctttggg
     1921 attagtctct cacttctgtt agtgaagtgt aattgtcttg gagtttttgc tggcggtcaa
     1981 cctcttttga ccagcgccct acttcacctt cgtaaccttt tttcttattt ttccaatcat
     2041 tttcagaagt ggctaaagca cgattgcgct gatcgaccag ggcgccttgt tgtttaattt
     2101 gctctccccg ggagacccat atcttttttt cttcctggat ggagttcatt tgggtttcat
     2161 aatcggtaag attggcatca cgttttgcga tattttcttg aagcttattc atggccgttt
     2221 gaaggtcttg cagcttcttt tcttccagtg cttttttatt tttttcctcg gccatcaagg
     2281 tatgcaattc tttttcctga gcttcaactt ttagcaaaaa ctttttattt tcttcatgct
     2341 gagagaccac ggtctttctt tgaccatcga cttgagcttt tgctttttga atttcaccga
     2401 tgtttttctc gacggatttg agattctttt gataatcgcc caagttggct tttgagttgc
     2461 tcacgttgct cttgattttt tccagcccct ctttcgccgt tgtttccgcg atggcttgaa
     2521 ggccgatcag taaagagccg ccaatgagga agctcgcgag cattttttta gagtttgttt
     2581 tattcttcat ggtcgtctcc tttaaattat tttttttacc aattagtttt catccaggct
     2641 ggtcactttg tcttctttag gacatacgtt ttttagagac gagcggtagt gacccacttc
     2701 atcttcccaa atctcgcctt ggaatttcca attcaggctc ttgtcagctt ccactttcaa
     2761 ttcaggacgt tctttttctg tgacgttgcc acctgccatc tgatagcgga tgtgctcgcc
     2821 cgctccagag tataattcat aagaaagaac ttcattttgt tcgagaacac gattcaaatt
     2881 cccggcaagt tgagtcatcc gtctttttaa ggctaaaccg gcttgctcgc gaagttcgct
     2941 tttctccttt tccatgcggg caatacccat ctcgcggagt tttttcatgg atgtacgggc
     3001 tttttgagca atgaattgtt gaattctaaa actcatttga cgtctctcag ctttggctaa
     3061 gctttcctga agagaagcgg attttttatt atctatgagt tttttacgga tttcagagat
     3121 ttcttttccg gcttcaacgg atttttttag catcatcttt tctcgttcca gcagagtgag
     3181 agtcactttg ttgaaacgtg aaatttcatc ttcagtcgtg ttgatttccg tttgcggaac
     3241 ggtgaaagaa gggtgacggg ccatttcaac gatgaaggga cggggtaaac catccacttc
     3301 tttgcgttcg atgtttttca tccatgcttt gatggtttca taatagtgag ccgggttctt
     3361 gtcattggca gattgatagc tggccagttt tcccttgaga ggggcgaatt tctttttcat
     3421 ttcatttaaa atctggattc catcgccata ttggcagagg ttcaaatagc ccacggtgcg
     3481 gacagtataa gactcaggcg caaacgcatt tttaaagaaa tccgtgtgaa gcgagaacat
     3541 attccccgcg gcaccttcat aatcttcagc catgatttgg gtccatgctt gttccgtcat
     3601 cgcttgcagc cacatggggt ttgacttttc cactttcaaa taagcctgaa aagaatcttt
     3661 gtaagcgcct ttttggaaat acatacggcc taaagtcaaa gctccgacat ttctaataag
     3721 gcctttttta tcgtcactgg tcagttctaa gagcttattc aaagtcatga tagcttcgtc
     3781 cactttgcct tgacgataaa gtgaaagagc gctgatcatg gtggcttcag gatattttgg
     3841 ggatttctcg ccgatgaaca tcaaagagtc ttcaacgagt tctaattggc ccgcttccaa
     3901 ataatattta gcccgagaca gctggtaggc ttcgttttgg gtggtatcta tgtcgtactt
     3961 ggtgatcaaa ggatctatgt actttatgtc agagaccttc aaggcagaga tattttcaac
     4021 gagttggcga acagcttttt cttgccattc tttgctttta gcatcggagg cgactttgat
     4081 catacccatg cggaactcgg agttaatacc caagcctttc gcggtgagag catagttata
     4141 agacgcttcg gtgcgatact cggggtcttc tatcagctca gaaaaaagtc ccagagccaa
     4201 ttcatagttt ttttggttcc ccaggaatat aagtgcttgc agcattttgt aatcgtttgg
     4261 tttcatttct tgcagcggca ttaattctgg ttttgcctcc accatatcca agaggggatc
     4321 agccacttcg ttcaagagtt ttagctctgg cacgttcgct atggcatttt taccggtgac
     4381 gaccctggat ccattggagg gaggaaattt tgtgagctcc agagggattc gcacctccgc
     4441 gggggaaggg cgtttcacga tcggattgag gcttaatttg ccagcttcgg gaatttgcag
     4501 aggcttgacg ttggcttgga cttctctttc ggcgccaatg ttaagacggg ggatttcggt
     4561 gactttcttt ttattttgca caagcttgta agcagggaca ttgctgcttt tcatatacat
     4621 atttgctttt tgagcatgag cggttcccgc tgccagacct gcagtcatca aagccaaaac
     4681 cagtttatgt tttttcatag gtcctccaca ggtcacccca gttgtctaaa accaataagt
     4741 cacacctaac aacatggacg tgtcgttggt catcttcgct cccagatcac gctctgttgt
     4801 atagttatga ctgccacccg aatttggcag gtagtatttt aaacgtttct gactggccca
     4861 agtgttggaa agatccgccc gcaaagcaaa gtgttcggag aagaaaatct gctggaacac
     4921 ggaaagcttg ccgaccgccg aactcacctc ttcattgcct tctacttttt gaatcgcgta
     4981 cttcaaagtt cccgcgccga cggagaaacc catatcaaag taaataatcg tgttatccat
     5041 gaagctcatt tttgcgtaaa agggaaccaa ggtaaaagag aggatttgcg aagacttaaa
     5101 gacattgtgg tctggataag tgccgtattg gttgatgaac tgggaagtcg catcattgtc
     5161 atgaaggctc gcatttttgt agtctaaatc gagaccccaa cgttcattga agaaatagcc
     5221 gaaagtcatg ccataaatat ttccagaact aaaggggtcg ttgaaaggca cgccgtagtt
     5281 ggcacttaag aaaaatcgat tggctttggt atagcgtctg ttttgcacaa ccgcaaagtc
     5341 atcatctttc gcggaccaat atttctgctc cagctttcga atatccagct tatcgttgcc
     5401 gccggtggtt gcagggctgg tttgtgcgta tgctagggag ctccctaaaa gcatcaacgt
     5461 gagtgtgaga gttttcatta tttctgctcc ttcgtattag ttagtactct tgaataagaa
     5521 tggataagtg acgagcactt ttgttcctcc cgcaggggcg gggaacttcc attttgcaac
     5581 cttattcaag atacatcctt ctacggtcgc atttttaagt gtggtatcgc caatagcctg
     5641 ggtttcaacc tgtccggtgc cggcgattgt aaacttcacg gccacctttc cgaagagttg
     5701 gggattggcg ctgagttgtc tttcatagca atagaggatt tgtccaagtt gggatttgat
     5761 atattgcgcg atcacttcgc gatctaagcc tcccaccact tcgctttcct cttcgatgag
     5821 gccgactccg ccattgcctg tattgccagc gccgaggccg ccgattccgg aagtgctttt
     5881 gccaccgcca cgtccagctg tggaaatcat caccccttga gctgtggagt ccgtgccgta
     5941 gtcacggccg gaacgatcga tattacccac cgccgctagc gcacggccac tggcgccaga
     6001 gcctgcttta ataccgttag agacaatcac attgccgctt ttagcggcct gtgcagaaac
     6061 ttttccgatg agacgggaaa tgcggggatc agcaacagct ttcaacatat ttgcaatttt
     6121 accggcgcct ttaggagctg aattctgggc agaatttttt tgaggctcct ttgcgggtgc
     6181 ggagggttga ggaacgggtt ggggaggtgc cggtttaacg gcttccgcca ctttcacttt
     6241 tttcttatga tctgttttta ccacgactct ttgagcgggt ggaggaataa ccacagtggc
     6301 catttgttct tgttttggcg ccatcaatga aagcccgcca atcatgaacg ccgccaagag
     6361 aagtataaga aatccttttt tagtatcttt atcaacaatc tggctcgctg taactcgatt
     6421 catcgcttct tcatcagtga gatagacggt tttttgccga acaatgtact gatcaagagt
     6481 gtattgaatc caagaatcag tgtcttttgc cggaataact tttttagtga tggccatttc
     6541 cggaataaaa ttttggccca tgggcaaaac ccgggtttca ataactttct cgtgaagctt
     6601 cacaagaaag agttgataag gtttcttgtc tttttcgaag ctaaggggat ttctttcgag
     6661 cttttgatag agatcacttt ctcgaagcaa gggggtgata tttaaagtgg agtcagctat
     6721 ttcaagggtt ttttcagctt ccagacaaat ttggcagtct gagcttgaca tgacattgtt
     6781 caagctcatg tcgatgtacc accattttcc atcgcggcat tcaaaagtcc cttgcattcc
     6841 ttgggaagtt ggggatatgg aaataacgtc ggcgtgccga gagcctccaa aggtgtagac
     6901 tggctgtgta gaatggagct tccaagtttt ggtagtcccg ttcttgagac gttgctttac
     6961 aatcaaaatg ttgttcatac gtaccccgta agtcttctct taaatatttt cttagccttt
     7021 tctccagtca ttgctcacta tcttagataa aggctggatt ctcgaatgcg tccgtccata
     7081 tctttttgaa tgtcgtgtaa aggcaaaaac ttaatacctt ttttttgtac gaggtaggcg
     7141 ccatcgggat ttcgaatcgt gcctttgagg ttcatttcgc cgaaattgac ctcttggact
     7201 ttacgggtga cctttgattt agccactgca tttgtaacga atcccaagac aacgattaat
     7261 cctaagatta attgtttcaa tgttcctccc taacaagtct tcggaattac agcagcgcga
     7321 ctttatttaa ctcaatttaa taactcttta aaaacttcaa atctagttgc tctctaaaga
     7381 agtgaccttt ccttcagtgg acccttgccc tttcagcagg gctaactttt taccaatcca
     7441 ttcttttaaa tccgccttgg tcgtgatcct ggcaattctt tcgtagactt gctgagcggt
     7501 tttcacgtcc gaggatttat aaacctcgat caaatgggcc tgttgtaaca ggatgtccac
     7561 attgtccttt ttcttagcgg acaattctgt gatgaactga aggccttttt caccttctcc
     7621 gcgcagtgcc caggcctcac tgaacaaagg tcccatgttg taattataca cctggtcttg
     7681 agtcaggcgt gagaaatttt ccgtcgaact aatataatct ttttctgtga aggctttgac
     7741 cgctgcaaaa gtcgctattt ctgatgaacc tatattcatg tctacaactt tggcgaaaaa
     7801 tggaatcgcc gcattgatat cttcgatctt atacatgagt cgacccttct gatacaatac
     7861 caaggcattc tcactttggg tcttcatcag ttcatcaacg agccacaagg ctttctcttt
     7921 ttgctcgcga gcatctgcgg ccaaactgag gtagtagagg cctaaggttc tggtgtcctt
     7981 gtatttggca agttctaaag agagcttctc ggtcacatca tattgttttg agatatagca
     8041 ggcattggca ttgtcgagga tggattgtat gctggcttcc ttgaaggtaa acgaacgatt
     8101 gcagactttg ctctggcctt tttgcacacg cacagggctc atatctgttt tggcagtcaa
     8161 atagccttca aactgagaaa caggcgggta gctcactgtc ggggaaatcg aagcctctgg
     8221 attgagttct gaataatcgc gacctttgcc gtcactggtc gcacgcacca ccatgagagt
     8281 ttttaaacgt tgctcgtttt ccgctttctt acccttaata ggggtggtga ttttagccag
     8341 ttctcctttg agagtctctt tttcatctgg agttaagccg cctggcatag gcatgttttg
     8401 taagctatcg atgtagtttg tgtagcaacg atcgagaccc gccaatgctt tgcgcacgac
     8461 tttttcatct ttacttaagg tcatggcatt cagataagcg gtttgtgcct tgtcgagttt
     8521 ttctgttttt aagccaagaa caacggcgaa tttatccatt ttggctttga cactttgttg
     8581 tatcagttcc gcctctaata cttgagcctg aattaaacgg gccggagctt tttcttcgct
     8641 gggtatatta cgtgccataa tttttttagc ggcatcaaaa gcgggagttt gtttgcctga
     8701 tattaaaagc ttttccgcac gctccgttaa aatttgtgtg gcataaggct ccatgttgat
     8761 agccaaggtt ttttcttgaa gcttttgata atccggatgg ttggtgtcat taccataagt
     8821 cgccatgatt ttggtgatca tgcgggcttg gtttttatta tccattcctt gcatgagctt
     8881 ggtatagatc tc
//
LOCUS       DATBTE010000407        27454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1491641, whole genome shotgun sequence.
ACCESSION   DATBTE010000407 DATBTE010000000
VERSION     DATBTE010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 27454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27454
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491641"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..685
                     /locus_tag="VIG33_15560"
     CDS             212..685
                     /locus_tag="VIG33_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8272308.1"
                     /translation="MSMSDENKKISVRLQGRDHIDVNLEGAFPKVSFRGCSEMMDLMQ
                     LMRKNFGIDLRQWPIPPGTSHSEILLRELILKLRGNWQYPTPEVEVCHCRSVACYTID
                     QAIIAGAHSTEIVSRQTSASTACGTCRSQVQKMIDYRLNEGRVAMTAPTPAKKSA"
     gene            complement(701..955)
                     /locus_tag="VIG33_15565"
     CDS             complement(701..955)
                     /locus_tag="VIG33_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY8272309.1"
                     /translation="MQKRKRAEIICRCNNIPRDVIEDAIKNEACDTLNKIFDATTAGV
                     GPCGGSCRRKLKPLLDHFLETGTFPEKIIEDLTGKVPAKK"
     gene            complement(979..1965)
                     /locus_tag="VIG33_15570"
     CDS             complement(979..1965)
                     /locus_tag="VIG33_15570"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEY8272310.1"
                     /translation="MTIVTELIKKSQKIILSTHRQCDGDGLGAELAIYYALKKIKKDV
                     RIVNVDSTPRKYRFLGSDKVISLFQNNPVIDLVADLCIIFDTNDERLLEPLFSGLKKN
                     IPKIVFIDHHPLLKKGPQPGTESYIDVTAASTGELAYGIIKELGIELDSTIARALYTS
                     ITFDTQLYRYVRNSPKSHLIAAELLLHDINPLEVHRALFGNQTMQKMAFLSKALGEIE
                     YFCHGRIAVLCLRDTDLLAHNIEPDEARDIIDIVMNIESLEASVTFREDAKNEYKLSL
                     RSKGKLEVLSIAEGFGGGGHTHAAGAFVKGIYVDLKEKIVKDLTKLISQIGL"
     gene            2136..2612
                     /gene="greA"
                     /locus_tag="VIG33_15575"
     CDS             2136..2612
                     /gene="greA"
                     /locus_tag="VIG33_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY8272311.1"
                     /translation="MADTLPMTVRGKALLDAELKKLMLEDRPDVIRAIEEARAHGDIS
                     ENAEYDAAKERQGMIEGRIGEIQGKIAGAEIINPAEIKSDRVVFGAHVTILDVETEEE
                     SKYQIVGVDEADVKKGMISILSPLARALVGKKVGDVVVVQSPKGDKEFEILGFEYK"
     gene            2678..4675
                     /locus_tag="VIG33_15580"
     CDS             2678..4675
                     /locus_tag="VIG33_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272312.1"
                     /translation="MMGPRTPRYPNDDKNLIAEQPGRLILFSGILVAVVLGLIVRGLT
                     APNKVKAMIESAASRMHKDVKVEFEGVQISLSRGLLPRFAVIITKVKMESANECWMKP
                     TLTADEVRLPLSLGALLQGEDPITQVEAGQVRIDLRSAYKNCEVHSSQPNEQAPKIKQ
                     FVTLKEVADSPKKTSASSQVRGILIDQLKIFTPNLPEPVELNSFEIRLKSNTPRVVEM
                     TAKTHLMKDEQVGDYLSHATVWGEYSEFPQSTLQTRISGNWREGSYHLKSTYGMKEEA
                     FTSEMELKHIPLSQAFQIFKKLQWLKEDLNGRQLWVSLNVQSSMTKSNLKTAQMHIKD
                     LRLEGDLGDLKVAETHVVSLDPVKYRPFTVDIRRLNAEKLFALLNRPHPAPMLGQLGT
                     FDGTAEVTDHDHIKLRGIHKGLEFIFSNKGQREVQVLKEVSAEISLDKSRWQIQVSHL
                     VPDQGVFDGQLAVNADRDLKSLEVKARANEIRLAPNVIRLMTAGGQMGAFNGDMTLRF
                     QEGQVNYIKGILNSESLDVEGVSTEKMRFNVDFADGEIQTQAQAQKMAIAVGSPAFQT
                     LKSLIEPEWMIENRLPMKNISSQLHFKNFKVLNWKNFSAQLEKGGRFVSEGQWNAEGE
                     LSGKLQTQVGKINHKWGLGGKRDEPIFTSVDLIKKKSNVAQ"
     gene            4662..5363
                     /locus_tag="VIG33_15585"
     CDS             4662..5363
                     /locus_tag="VIG33_15585"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY8272313.1"
                     /translation="MLLSEKKFQTHRGFWIEGRMENTLASLSAAKKRGSEMVEFDVRL
                     TSDYVPVLYHDENLRRLHKAPVNMQGLTLQQARVFAPNLTTLEEVLESKEVPDYLNIE
                     LKTDSTVDPAFEIRVSQLIRKFKAEERVVLSSFNPFSLIRARGLIPEVSRALLVSEDE
                     EPKNYWFLKQMTMLPLCEARFLHWDQKMTSRERVRKFRDVGYEIAVYTVNDKARAKEL
                     FSWGVHSIISDSLLA"
     gene            complement(5410..5667)
                     /locus_tag="VIG33_15590"
     CDS             complement(5410..5667)
                     /locus_tag="VIG33_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEY8272314.1"
                     /translation="MGEFSLTHLLLLAVIFLIFFGPSRLPQLGQSLGKAIRGFKTGLS
                     EIDVDVKDIHDTPKTNDQISQNQAQGMNSQKVETPETQKKS"
     gene            complement(5747..6280)
                     /locus_tag="VIG33_15595"
     CDS             complement(5747..6280)
                     /locus_tag="VIG33_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272315.1"
                     /translation="MKVLANHILFLTFTFWTVIGHSQQLSSELTCRTKAKEIATQTYS
                     GCVTEARNSQIDQIRKDYQKQLTELKAKYDKELKKVSGKGLTAVTTAATGSLPKAAKG
                     VAKTLPTRKEVKNVAPPVSQIAQEDKTVTIPDPSTPASGGDESTQNDSKSEPKDSLTV
                     PAASSDQIFTSSEGQTY"
     gene            complement(6404..7603)
                     /locus_tag="VIG33_15600"
     CDS             complement(6404..7603)
                     /locus_tag="VIG33_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8272316.1"
                     /translation="MIEEITIAPLSDANRKKLEQALPMWKIVLSQFLEHKMAVGGAVI
                     IFCFMILALSAPLIQTLTGIDPDAQNVFNRYQAPFSSAIAGQDIRETAIEKFRTEHPE
                     IAESLEKSLIDKSLVTTTTPADALFELTTKDVPEAFEILEQVGTNQAKELQQTFKTFS
                     TYHLFGTDELGRDVFIRLIYGTRISMSVGVLVAIVSALAGLLIGSLAGFYGGAIDTIL
                     MRLTDALLSLPLLPVMIVIAAIDLQKLPLLKSLVSQQNESILKMILILCLFSWMTVAR
                     LVRGSILSLREREFILAAKTLGATDFTIILRHMFPNVIAPMLVSITLGVGESILFEAA
                     LSFLGLGIQPPTPSWGNMLFNAQELIYQAPFLAILPGIMILLTVISFNYLGDGLQDAV
                     DPKAIRR"
     gene            complement(7629..8600)
                     /locus_tag="VIG33_15605"
     CDS             complement(7629..8600)
                     /locus_tag="VIG33_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8272317.1"
                     /translation="MTTYILRRLIQTCLVIAILSYVCFYLMTLMPGDPVDLMASSNPK
                     ITAEDVIRLKQLYGLDQPSYVRYGHWISEIAQGDLGYSRTYRVPVTDLLGPRLLNTFI
                     LSMVALGFSLMIAIPVGIISALRPGSKMDYIFNFFSFAGISIPSFWLGIVCIIVFAVW
                     IPLFKAGGTQTIGGPEFGFWGAILDRSKYLVLPVLSLSLQQIGRFSRFTRSSMLEALR
                     NDFIRTAKAKGLPRQVIIWKHAFRNALIPLITILALSMSSIFSGAIITETVFAYQGVG
                     KLTYDSIIGNDFNVAMVSFIISVAMVLLMNLVADLLYGVADPRISYT"
     gene            complement(8614..10332)
                     /locus_tag="VIG33_15610"
     CDS             complement(8614..10332)
                     /locus_tag="VIG33_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY8272318.1"
                     /translation="MKAIFTLSLLAILSIVGSATIAMSATSAPNNTELKIGISQEFEN
                     FNPLIKTMAATSYMLSLADRTLVSLDSNGKWYPQLAKSIPSLENGGAKIVDVGGKKKI
                     IAIWEIIDAAKWGDGKPVICEDFALALKVAAAPTVSVGEKETYTLVEKIEADPKNPKK
                     CTFTYEKARWDFFQLGQFAPLPKHIEESVFNKHGKEKEGYEKNSNYSKNPSMKGLYNG
                     PYVISEVKLGDHVTFVPNPYFYGPTPKIQKIIFKLIPNTGTMEANLRSGTIDMISTLG
                     LSFDEAVTFSKKVKAENLPYDVTFTPSVTYEHVDLNLDNPILKDIKVRKALLYGLNRE
                     DLVKALFDGKQQVAVHSISPKDSWFTKDPKDIVLYPYSKREAGKLLDEAGWKMGTDGF
                     RSKDGKRLSLVFMTTAGNKVRELVQVYMQNQYKQLGIEIITKNEPARVFFGETMRKRN
                     FGSMAMFAWVSNPENSPKSTFHSTSIPTDKNGWSGQNFMNWSNKKSDKLMEDLELEFN
                     PQKRAEMVHQILKFYTDEVPVLPLYYRSDTSVVPKNLKNYKVSGHQFAETNEAEKWDL
                     GSTTLN"
     gene            complement(10329..11366)
                     /locus_tag="VIG33_15615"
     CDS             complement(10329..11366)
                     /locus_tag="VIG33_15615"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEY8272319.1"
                     /translation="MQEVLLEARNIKKYFPIKKGLLMREIGQVKAVDDVSLSVKRGET
                     LGLVGESGCGKSTLGRTLIRLYEPTGGDLTFKGKDFLGLTGQELRQKRKNIQMIFQDP
                     YAALDPRMTIGQIISQPFQIHKTLDSKEAEIRVKELLELVGLKASHINRYPHEFSGGQ
                     RQRICIARAIALNPELIICDEPVSALDVSIQAQILNLLKDLQEKLKLTYIFISHDLSV
                     IEHVCDRVAVMYLGKIVEIATRDELFANPQHPYTQALLGAIPQVGEGKKQMKRSLVGD
                     VPSPINPPSGCHFHPRCAYMMDICSKERPQLKGSDHQASCWLVEQNKSFARTLDPSLK
                     PNIETSKKESP"
     gene            complement(11378..12352)
                     /locus_tag="VIG33_15620"
     CDS             complement(11378..12352)
                     /locus_tag="VIG33_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8272320.1"
                     /translation="MFALPDPVLSVRNLETTFKSKLGPIRAVNKVSYDIHKGQTLGIV
                     GESGCGKSVTSFSIMRLIEPPGKISGGEVLLKGRDLLTLSEKEMEEVRGGEMAIIFQE
                     PMTALNPVLTIGYQMDEQIKRHMKLSSKDAKDRAIEMLSLVGIPSPADRYATYPHQLS
                     GGMRQRVMIAMALSCNPSFLIADEPTTALDVTIQAQILELFQSLQDKLHMTVQFITHD
                     LGVISEVSDRVLVMYGGQACEKATTLELFLNPRHPYTSALIESRPRLGHRANRLKTIE
                     GSVPAPMDLPAGCPFQNRCPRAKSECLTHKPPMTKIGPDHEVACFNPL"
     gene            complement(12459..13469)
                     /gene="sohB"
                     /locus_tag="VIG33_15625"
     CDS             complement(12459..13469)
                     /gene="sohB"
                     /locus_tag="VIG33_15625"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEY8272321.1"
                     /translation="MDFLQQFGLFTAQTVVIVLAIASVLLLIALLAAKAQDKAEIEIE
                     LLHKKYKMFAKILKNATLNKEERKEETKKEKKDLKEKKKEHQRKIFVMDFDGDIKASA
                     VEHLRQEVTAVMNVATPQDEVVIRLESPGGVVHGYGLAASQLLRFREKGIPLTVCVDK
                     VAASGGYLMSCTANKILCSPFAIIGSIGVVAQVPNFHRVLKKYDVDYKEYTAGEFKRT
                     VSLLGEITEKGEEKFKEQLEATHRLFQGFVQKNRPSVNLGKVATGEYWYGQDALDLGL
                     VDEIRTSDDYLLSQSDTNQIIKVSFAKKQKLADKLSGILGRAASSALLKVYEDLERKT
                     LL"
     gene            13616..14350
                     /locus_tag="VIG33_15630"
     CDS             13616..14350
                     /locus_tag="VIG33_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272322.1"
                     /translation="MFLRFIIFFLISVFITPHSLAQSEEERRKGFYEHQKENKKFEQE
                     REAGLKDYLKEQAEWEEQRKKDTLADKKNKKQETPAENSAEYRADRKGKYEDYEEYEK
                     ARKAYVKEKKSHEAKNAKEQAKREEWALEEYNLDKERPRFDIAKRNLLGGKSGGSPGG
                     GSSGGIGGGAPNFPAPPAFDDFSDGYVPPPFPPPESFEPSPESFPPPPPIPFPGEAGP
                     GGDFDGGYYPPPPPPPSMPMPPGGGF"
     gene            complement(14355..15068)
                     /locus_tag="VIG33_15635"
     CDS             complement(14355..15068)
                     /locus_tag="VIG33_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HEY8272323.1"
                     /translation="MEKKQQQKPENSLLNILFNIIIPILILNKGSQVLGSFWALLIAL
                     SFPLSYGIYDHIKRKKTNAISVLGILNVGITGTLALLKLHGMWFAVKEAAFPLLMGSF
                     VFGSAFTKDPFISTLFLNPQLIEVEKLKSRLLERNTETEFHHLLKKATLWISLSLLFS
                     AICNFFLALRIFEPINESLDAEAQASILNEQIAHMTTWSLGIIVLPSVLFLVAIFLYL
                     TKGMQRLSGLKEDELLVQK"
     gene            15386..17863
                     /gene="thrA"
                     /locus_tag="VIG33_15640"
     CDS             15386..17863
                     /gene="thrA"
                     /locus_tag="VIG33_15640"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF007003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HEY8272324.1"
                     /translation="MNHKQQWKIHKFGGTSVLNSDRYKNVFLIVSSLVPSSRKAVVVS
                     AMKGVTDDLIKTVEMAQHQQESYKNLLESIYQRHCTEIKALLGAPARDALLAVIERDF
                     HELREILRGVWLVRTASNQIYDLVSGMGEVWSAQILNAYFQLQGVKTDWLDARKILVI
                     EKTEQAVVINWRASQENLTRWQKEHDYDLVTITGFVAATVDGTIATLGRNGSDYSASV
                     FGALFDCDEIFIWTDVDGIHSADPRQIPEATILKEMSYNEVTELAYFGAKVVHPATMV
                     PAIKKQIPIWIKNTFNPTCLGTKIFQDAESSAPVKGFSTIDKLSLLNVEGTGMVGVPG
                     VAERLFGSLRSSGISVVLISQASSEHSVCFAIPEEQTEKAQKAIELAFFAEIQQGLIQ
                     NVQVTRNVSIIAAVGDNMACSPGTAGRFFTALGRSGVNVRAIAQGSSERNISAVIDTK
                     EAIRALRTVHSAFIVPHQTLSVGLIGPGLIGRTFLRQLKEKQLDLKKGREVDVKIRAI
                     ANSKMMYLSEKEIPLNQWEELFAKNAVPLDWNKFTEHLKPSHLPHAAVIEATASSSLT
                     PLYKEWLAQGLHIISPNKKANTDSMDEYLKIRAAAKRYSRHFLYSTNVGAGLPIVQTL
                     RDLIATGDEIKIVQGILSGTLSYIFNQYDGTQPFSEIVKKAKEMGFTEPDPREDLSGQ
                     DVVRKLVILARETGMNLEVDDVEVQGLVPSALTAISVDEFMSRLAEMDAPMSEKMKNA
                     QAQKQILRFVATLSQDGKARVGLEALPAQHVFSRVSGTDNIVLFKTKRYFEQPLVIQG
                     PGAGPEVTAAGVFADLLRLSQYLGALP"
     gene            17875..18825
                     /locus_tag="VIG33_15645"
     CDS             17875..18825
                     /locus_tag="VIG33_15645"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY8272325.1"
                     /translation="MKKVVMKKSVARAFAPATVANVAVGFDILGFALSGLGEVATVRK
                     INLPGQVKVHPVAGFPMLPVNPLKNTCSAGLLQLLKDKDLPFGFEVSLKKSIPIGSGL
                     GGSSLSACASIVAANALLQKKLSKAEVLHYALIGEAVASGAIHADNVGPCLEGGLILV
                     RQHPEISLVKVKTPSSLRCVVILPELSISTQEARSLLTPQVPLKAMIEQTANLAGFLI
                     GCVAKDFKLMGESLRDVVIEPQRAKLIPNFADLQAAALDAGALGCSISGSGPAVFALA
                     KNQTSALKIRRALLAVSAKKKLQLRGSWISPIASQGAHLL"
     gene            18822..20096
                     /gene="thrC"
                     /locus_tag="VIG33_15650"
     CDS             18822..20096
                     /gene="thrC"
                     /locus_tag="VIG33_15650"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY8272326.1"
                     /translation="MKWVSTRGDSTLFSTSEALKQGLAQDGGLLVPQVDPEIKFDPSW
                     GELPFSQFAEKVLRPFFVGDILEESLPAICQEAFNFPIPLEALSEDTSLLELFHGPTS
                     AFKDVGARFLALCLAKIPDARDKVVLVATSGDTGGAVAAAFCECSDIPIAVLYPKGKI
                     SQRQEKQLTVWGKQVQAFAVRGSFDDCQRIVKEAFLSKAWQKRFQMISANSINIGRLL
                     PQMVYFAFASLKCQLKSQLKNHSKPTIIVPSGNLGNGVGALWALRLGFPIQQVIFAHN
                     ANQAVPQYFAEGAWSPQSTIATLANAMDVGNPSNFERLRYFYPNLNALRKVASAHSVS
                     DAQIRQTIQEQASKGWILCPHTATAVYVRELYKERSCIVVATAHPAKFETIIEPLIGK
                     TISIPQNLNLLLSKPSVFKEISPDLASLHIII"
     gene            complement(20166..20942)
                     /locus_tag="VIG33_15655"
     CDS             complement(20166..20942)
                     /locus_tag="VIG33_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HEY8272327.1"
                     /translation="MLYGNESISHYTKSNISIWMVMAFQAGVLNIGGFMACQRFVSHV
                     TGFATFFGYDVSQGQPRHALGMLVVPLFFLLGAMVSGQLVDLRLKLHKKPRYYLTFGV
                     IFFLTLFVTIAGVLGRLGQFGESLTTSENYTLLILLCFICGIQNGTITTVSKSVIRTS
                     HLTGITTDLGIGLVRLLNQRKLADHIGNEKKATLMRIGIIFFFGFGSVIGGFAFSSFG
                     HAAFLIPVLTSGVLFWTMIYFQVWNQPKEEELPYKKKPVH"
     gene            complement(21120..22412)
                     /locus_tag="VIG33_15660"
     CDS             complement(21120..22412)
                     /locus_tag="VIG33_15660"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY8272328.1"
                     /translation="MKKRLSLMSWSVSRPVSWPVSWPASFSLIFALSMEAHAIEGFFS
                     LEQQLNEQNQTLSALKKELQSKESLLQSSYSPLYPSLSAVAGWGQNRMDNPEESNQGY
                     FGYLEGRLNLFNGLKDIAISDQKEIEVQLKKLEYENSRLEIRLALAETMGNMIYLHKL
                     QTILNDEARITKDQKMMAAKKVSSGLTSSVDNLELDLREKEIEIQQKQIDQLHRESHA
                     KLFELFGQDIPDTELDKMTFDSIEKLRYVKSFNEQNNIEVQRAQLVLILSHLEQKELR
                     ADFMPSVDFVYSFGRLTPSETSPVKMNESKYGIQVSIPLFSGFSTIHKNTAGKYETLA
                     KKSRATQAVLSSQSSFNSLKEKLQELSDLYEINEAKLVTSKKYFEMTVSEYKRGIKNS
                     PDLVGATERWFNSQKKKLELLKELELTKTRIENLNGNS"
     gene            complement(22435..22827)
                     /locus_tag="VIG33_15665"
     CDS             complement(22435..22827)
                     /locus_tag="VIG33_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272329.1"
                     /translation="MKKILLSMSAILFLQPALAHEGHDVAPGALKALHGGSVLAGKEI
                     NLEYIVTSNQVKLYPLSHEGKDLEPSQIKVKATAKAPRGKSENLKVELKEGALTTQVD
                     FKNAYRVEVNVNTETNGKKDSFKFQVEK"
     gene            complement(22858..26001)
                     /locus_tag="VIG33_15670"
     CDS             complement(22858..26001)
                     /locus_tag="VIG33_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY8272330.1"
                     /translation="MLDRVIRFSLTHRLWILVLAAVLTAYGLFTLSRMPVDVFPDLNR
                     PTVNIMTEAPGMAPEEVETLVTLPLETVLNGLPGVERVRSTSGIGLSVIYVEFNWDTD
                     IYRNRQLVSEKLQLAKEKLPRGATPIMGPISSIMGEIQLIGLSIAETSPDTKVNPLDL
                     RTYADWTLRPRLLAIPGVAQVIAIGGGVKQYQILLSAEKIQKSQIPLADIEHALSNIS
                     LNTTGGYIDIDKKEFLIRNIGVVRSEEDIQNTVVGLHLGKPVFVKDIAEVKIGAQSKR
                     GDGSVNGRPAVILSVQKHPGTNTIELTKTIDVALKDLESSLPPGVQLTKELFKQAHFI
                     EAATDNVKEALRDGSILVFIVLLFFLMNFKTTLITLTAIPLSFLITAIVFHFFGLSVN
                     TMTLGGLAIAIGELVDDAIVDVENVFRRLRENRQLPAPLPTLQVVYNASSEVRNSIVF
                     ATVIVVLVFLPLFSMSGIEGRLFRPLGLAYIISLISSLFVSLTVTPALCSLLLSKDAL
                     REHGDSKLVKKLKQWDRQILTKALDHPQIIIGATLALFLASLLLIPLMGKDFLPKFNE
                     GTATVSVLAQPGISLEESNHLGTQVERALLSIPEIKSLARRTGRAELDEHAEGVHSSE
                     IDVDFKDKGRSRETVLNEMRDKLKQIPDIFVNIGQPISHRVDHLLSGVRAQVAIKIFG
                     HDLSILRTKASDAFKALEGIPGLVDLQVEQQVLIPQVKIQLLRTEAAKYGMILGEISE
                     TLEKALKGEVISEILDQQKTFNVYMRFDDHSRANLEVMKKTALKIMPDGTKVTLEKVA
                     DVYESQGPNQINRENAQRRIFVSANSSGRDLDSLVLEIQQRIKQKVSLPEGYFIEYGG
                     QFESQRLASRLILFLGIISLLGVFIVLFFHFKSFFIAVQIMLNIPMALIGSLLAIYLS
                     NRSFSIATMVAFVTLCGIASRNGIMMISHYLHLMKHEGEVFTKDMVIRGSLERLVPVL
                     MTTLTAILGLLPLVLAKGDAGKEILHPVAVVIVGGLISSTLLDMFVTPTVFYRFGKKS
                     AEKNIKTIETELKPTPH"
     gene            complement(26033..26461)
                     /locus_tag="VIG33_15675"
     CDS             complement(26033..26461)
                     /locus_tag="VIG33_15675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272331.1"
                     /translation="MKHLICAVTILFSLNAFAHGEDKLGPNGGFIQMPGAYHTELVPD
                     GTHKLKVFFLDISWQTQGLNDSSVEIIYKGKKTLKSACEPQKTQDKSFFECIFPKDAD
                     LTKNGKLIVTSQHNKQKGNDVTYPLPLKLQNTSESPGSHH"
     gene            26663..>27454
                     /locus_tag="VIG33_15680"
     CDS             26663..>27454
                     /locus_tag="VIG33_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY8272332.1"
                     /translation="MSLKISRILHAGYVFECDETQIAFDPIFENPFSRNCYAFPSVRF
                     DHEQITKVKFAAVFISHFHDDHCSLESLVLLDRKTPIYIYCIFEELFSLIRALGFTHV
                     YSLEMNVPVVIGSLEIIPWRALDADVDSIFQIKAAGLNVLNVVDSWIDFATLSELARS
                     APWDMVLWPFQTMREIEVLAPSRATVATGELPTEWIEQLKILNPKYVVPSSCQFIQES
                     WSWYNHAFFPISYRQFQKEIEDALPDSQVVRMNPSVAVVLDKHMLA"
BASE COUNT         7516 a         5718 c         6544 g         7676 t
ORIGIN      
        1 tctattgaaa tattttcgga aatagtttca gaaatttttc tagaaatgtc ttcccaaggg
       61 ctttctggat tctcgataaa ggccctgtgt ccacgatttt tgatttcctc gaccaaacgc
      121 cgggaggagt aaatatgggg aagacgactt aatataagaa agttcattcc tagcctttct
      181 cttcaaaggg agttgattag aatataccca tatgtcaatg tcagatgaaa ataaaaaaat
      241 cagtgttcgc ctgcagggtc gcgatcatat agacgtgaat ctggaggggg ccttccccaa
      301 ggtgagtttt cgaggatgca gcgagatgat ggatctcatg caattgatgc ggaaaaactt
      361 tgggattgac ttgcgtcagt ggcccattcc ccccggaacc tctcattctg aaattttatt
      421 aagagaactc attttaaagt tgcgcggaaa ttggcagtac cccactccag aggtggaagt
      481 gtgtcattgt cgcagcgtcg cttgttacac gatcgatcaa gcgatcattg caggagccca
      541 tagtacggaa atcgtcagcc gtcagacctc agccagtacc gcctgtggga cctgtcgctc
      601 ccaagttcaa aaaatgattg attaccgtct gaatgagggg cgggtcgcga tgacggctcc
      661 gacacctgct aaaaaaagcg cttagctgtg caaagtgagt ttatttttta gcggggactt
      721 tcccggtgag gtcttcgata attttctctg gaaaagtgcc cgtctctaaa aagtgatcca
      781 gtaaaggttt taattttctt cggcaggagc ctccgcaagg acctacgcca gcggtggtgg
      841 catcgaagat tttattgaga gtgtcacaag cttcattttt aatggcatct tcgataacat
      901 cccgaggaat attgttgcag cgacagatta tctcagcacg ttttcttttt tgcatggaat
      961 tcttatatgg aatcttggtt ataaaccaat ttgtgaaatc aacttggtta aatctttcac
     1021 gattttttct tttaagtcca cgtatattcc tttcacaaag gcgcctgcgg cgtgagtatg
     1081 accccctccg ccaaaacctt cagcaataga gagaacttct agttttcctt tactgcgaag
     1141 gctgagctta tattcgtttt tcgcatcttc tctgaaagtg actgaagcct caaggctttc
     1201 aatattcatc acaatatcaa tgatgtcgcg ggcttcatcg ggctcgatat tgtgagctaa
     1261 caaatccgta tcgcgaagac aaaggaccgc aatacggcca tggcaaaaat attcaatttc
     1321 accgagagcc tttgataaaa aagccatttt ttgcatcgtt tgatttccaa aaagggcccg
     1381 atgcacctcc agaggattga tgtcatggag taataattcc gcagctatta aatgggactt
     1441 gggcgaattt cgaacgtagc gatagagttg agtgtcaaag gtgatactgg tgtaaagggc
     1501 tctggcgata gtggagtcta actcaatacc gagttccttg atgataccgt aagccagctc
     1561 cccagtggag gcggcggtca catcgatata ggattcagtt ccgggttggg ggcctttttt
     1621 caaaaggggg tggtggtcga taaagacaat ttttgggata tttttcttaa gtccggaaaa
     1681 aagaggttcg agaaggcgtt cgtcattggt atcgaagata atgcagaggt cggccaccag
     1741 atctatcaca ggattatttt gaaaaagaga aatgactttg tctgaaccta aaaagcgata
     1801 tttgcgagga gtgctgtcca cattgacgat tcgaacatct tttttaatct ttttgagggc
     1861 ataataaata gccagttcag caccgagacc atccccatcg cactgtcgat gggtagaaag
     1921 aatgattttc tgtgattttt tgataagttc cgttactatt gtcaaaatgc acctagcctt
     1981 taaacaagct ttattttagt aatatttaca ggctctggat acccagccta gtctcaaact
     2041 agacagctta acctgctgcg cagcactaat ttcttgctgt ttaggtccag tttaagtaaa
     2101 atactttctc tcattttttt aaaggagttg atttcatggc agatacactt ccaatgacag
     2161 ttcgtggtaa agcgttactt gatgcagagc ttaaaaagtt aatgcttgag gatcgtcctg
     2221 acgtgattcg tgctattgaa gaagctcgcg cacatggcga tatcagtgaa aatgcagaat
     2281 atgatgccgc taaagaacgt cagggaatga ttgaaggccg cattggcgaa attcaaggca
     2341 aaatagcggg tgctgaaatc atcaatcctg cagaaattaa gtcagatcgg gtggtttttg
     2401 gtgcccatgt caccattctg gatgtggaaa ctgaagaaga gtctaagtac caaattgtgg
     2461 gcgtagacga agcggacgtt aaaaaaggca tgatttcaat tctatcgcca ttggcccgtg
     2521 ctttggtcgg taaaaaagtc ggtgatgtgg ttgtggtgca aagcccgaag ggtgataaag
     2581 aattcgaaat ccttggtttt gaatataaat aagcaatctc ccaagaattt tgtgaaatct
     2641 cattgtcggc tccccggagc ttcgtcaaaa tgaagggatg atggggccac gtacaccaag
     2701 atatcctaat gatgataaaa atttaattgc cgagcagcca gggcgcctaa ttctttttag
     2761 tggaatcctg gtggcggtgg ttctcggttt gatcgtgcgt gggttgaccg ctcccaataa
     2821 agttaaagcg atgatcgaga gcgcggcctc tcgtatgcat aaagatgtca aggtggagtt
     2881 tgagggcgtt caaatcagtc tttccagagg acttcttcct cgttttgcag tgataattac
     2941 caaagtgaaa atggaatcag ccaatgagtg ttggatgaag cccacgctta cagctgatga
     3001 agttcgtttg ccgctctctt tgggcgccct ccttcaagga gaagatccca tcactcaagt
     3061 tgaagcgggg caggttcgta ttgatcttcg tagtgcttat aaaaattgcg aagttcactc
     3121 ctctcaaccc aatgagcaag ctcctaagat taagcaattt gtgactttga aagaggtggc
     3181 ggattctcct aaaaaaactt cggcttcttc tcaagtgcga gggattctaa ttgatcaatt
     3241 gaaaattttt acccccaatt taccagaacc ggtggaattg aattctttcg agatccgttt
     3301 gaagtccaac actcctcgcg tggttgagat gaccgcaaaa actcacctga tgaaagatga
     3361 acaggtgggt gattatttat ctcatgccac tgtttggggg gagtatagtg agtttcccca
     3421 gtccacactc cagacgcgta tctctggcaa ttggcgcgag ggaagttatc atcttaagtc
     3481 gacttatgga atgaaagaag aagctttcac ttcagagatg gagcttaaac atattcccct
     3541 aagtcaggct tttcagattt tcaaaaaact tcaatggctg aaagaagatt taaatggtcg
     3601 ccagctatgg gtttctttga atgtccaatc ctcaatgact aaatccaatt tgaaaaccgc
     3661 ccaaatgcac attaaagatc tgcgtctgga aggagactta ggggatttga aggtggcgga
     3721 gacccacgta gtttctttgg accctgtcaa ataccgtccc ttcacggtag atatccggcg
     3781 tttaaatgcg gaaaaattgt ttgccttgtt aaatcgtccg catccggcgc caatgctggg
     3841 tcaactggga accttcgatg gaacagctga agtgacggat catgatcata ttaagctcag
     3901 gggtattcac aagggattgg aatttatttt ttccaataaa ggtcagagag aggtgcaggt
     3961 tttgaaagag gtgtcggcgg agatctcttt ggacaaaagc cgttggcaaa ttcaagtttc
     4021 tcatttggta ccggatcagg gcgtatttga tggtcaactg gctgtgaatg cggatcgaga
     4081 tttaaagagt ttggaagtga aggccagagc aaatgagatt cgcttagcac cgaatgtgat
     4141 tcggcttatg accgccggtg gccaaatggg tgcgttcaat ggggatatga ccttgcgatt
     4201 tcaagagggg caggtcaatt acattaaagg aattttgaat tcggaatctt tggatgttga
     4261 aggggtcagc actgaaaaaa tgcgttttaa tgtggacttc gctgatggag aaattcaaac
     4321 ccaggcgcaa gcacaaaaga tggccatcgc ggtggggtct ccggcttttc aaactttaaa
     4381 gagtttgatt gaacccgagt ggatgataga aaatcgtttg ccaatgaaaa atatcagttc
     4441 ccagcttcat ttcaaaaatt ttaaagtgct caactggaaa aacttttcag ctcaactcga
     4501 aaaaggcgga cgatttgttt ctgaggggca gtggaatgcc gaaggggaac tctcaggaaa
     4561 attgcagact caagttggaa aaataaatca caagtggggt cttggaggta agagggatga
     4621 gcctattttt acttccgtgg atttaataaa gaagaaaagc aatgttgctc agtgaaaaaa
     4681 agtttcaaac acaccgggga ttttggatag aaggtaggat ggaaaatacc ttggcatcac
     4741 tttctgcggc gaaaaaacgc ggcagcgaaa tggtggagtt cgatgtgcgt ttgaccagtg
     4801 attacgttcc ggttctttat catgacgaaa accttcggcg tctccataaa gctcctgtga
     4861 acatgcaggg gctgacgctc caacaagcga gggttttcgc tcccaatcta accactctag
     4921 aagaagttct agaatccaaa gaagttcccg attatttaaa tattgaactc aagaccgact
     4981 ccactgtgga tccagctttt gaaatcagag tctctcagct cattagaaaa tttaaggcgg
     5041 aagaacgtgt cgttctgtcg agcttcaatc ctttcagttt gattcgagca agaggactta
     5101 ttccagaagt ctctcgggct ttgctggtga gtgaagatga agaacctaag aattattggt
     5161 ttttgaaaca aatgacgatg ctgcctttgt gcgaagctcg ctttttgcat tgggaccaaa
     5221 aaatgacgtc ccgggaacgt gttcgaaagt ttagggacgt tggttatgaa attgctgttt
     5281 acaccgtaaa tgataaggca cgggccaaag aattgttcag ctggggcgtt cacagcatta
     5341 tttctgattc cttattagct taatgtactg gcagcaaaac gcccttatcg ggggcgtaag
     5401 gcgaaagaat tatgattttt tttgagtctc aggtgtttca actttttgag agttcatacc
     5461 ctgtgcttga ttttggctga tttgatcgtt ggtttttggt gtatcatgta tatctttgac
     5521 atcaacatca atttcgctga ggcctgtttt aaagccccga atggcttttc caagggattg
     5581 acccaattga ggcaaacgac tgggaccgaa gaatattaaa aagataacag ctaaaagtaa
     5641 aagatgcgta aggctaaact cacccacaaa gacctccaat taggatctag atccattgta
     5701 tattatatgg gtgggttttt gtcacgaaat aagcgatttt ccttggttaa taggtttgcc
     5761 cctcagaact tgtgaatatt tggtcggaag aggccgcagg gactgttaat gagtccttag
     5821 gctcactttt ggaatcattt tgagtcgatt cgtcccctcc actagccgga gttgaggggt
     5881 cgggaatggt caccgtttta tcttcttgag ctatttgact cactggcgga gctacgtttt
     5941 taacttcttt acgggtcgga agagttttgg ccactccctt ggcggcttta gggagcgagc
     6001 cggtcgcagc agtcgttaca gcggtaagac ctttgccact tacttttttc agttctttat
     6061 catacttggc tttaagctct gttaattgct tctggtaatc tttacgaatt tgatcaatct
     6121 gcgagtttct ggcctcagtg acacaacccg aataagtctg cgtcgcaatt tccttagcct
     6181 ttgttcggca cgtgagttct gaagatagct gttggctatg tcctatgaca gtccaaaatg
     6241 taaaggttaa aaataagata tgatttgcta aaactttcat aagcacctct tgtctgtgtg
     6301 aagacttaca aaagacatgc ccataaacag agatctcagg ggtcattaaa attgaaaagg
     6361 cctccaagag gtcttacttt tttacataaa tgtacagcaa ttatcagcga cgaatggctt
     6421 ttggatccac cgcatcctga agtccatcac cgaggtaatt aaaactaatc accgtgagaa
     6481 ggatcatgat ccccggtaag atcgcaagga agggagcctg ataaataagc tcttgggcgt
     6541 tgaacagcat atttccccaa ctgggagtgg gaggttggat gcctaaaccc aagaagctga
     6601 gagccgcttc aaataaaatg gattccccaa ctcctaaggt aatggaaacc agcatcggcg
     6661 caatcacgtt tgggaacata tgcctgagaa taatcgtgaa gtcggtggcg cccaaggtct
     6721 ttgccgccaa aataaattcg cgctcacgca acgaaagaat gctcccacgc actaaacgag
     6781 cgacggtcat ccaagagaat aagcaaagaa tgaggatcat cttaaggatg ctctcatttt
     6841 gttgactcac cagtgacttt aacaagggca gtttttggag atcaatagcg gcgatcacga
     6901 tcatgactgg aagtagtggt agagataaaa gcgcatccgt cagacgcatg agaatcgtat
     6961 cgatggctcc tccatagaag ccggcaagac ttccgatcaa tagacccgcc agagccgaaa
     7021 caatcgcaac cagaacgccg acgctcatgg aaatacgagt gccataaatt aaacgaataa
     7081 aaacatcgcg gcccaattca tcggtgccaa agagatgata cgtcgaaaag gttttgaagg
     7141 tctgctgaag ctcttttgct tggtttgtgc ccacttgctc cagaatctca aaggcctcgg
     7201 gaacgtcttt tgtcgtgagt tcaaacaacg catccgccgg agttgtcgta gtcaccaaag
     7261 atttatcgat taaagatttt tcgaggcttt cggcgatttc tgggtgctcc gtgcggaatt
     7321 tttcaatcgc tgtttcacga atatcttgcc ccgcgatggc gctggaaaac ggagcctgat
     7381 agcgattgaa gacgttttgg gcatcggggt ctatcccggt gagggtttgg ataaggggtg
     7441 ctgacagcgc taaaatcata aagcaaaaaa taatgacggc acccccaaca gccatcttgt
     7501 gttctaaaaa ttggctaaga acaatcttcc acatgggcag agcttgctcc agctttttgc
     7561 ggttagcatc tgagagaggg gcgattgtta tttcttctat catttccaca tttgctccaa
     7621 cactctgctt aagtatagct aatgcgtgga tctgccacgc cataaagtaa atccgccact
     7681 aagttcataa gcagaaccat agcgacagag ataataaagc tgaccatggc gacattgaag
     7741 tcattgccaa taatagaatc ataggtcagc tttcccacac cttgataggc gaaaactgtt
     7801 tcagtaataa tcgccccaga gaatatagag gacatactaa gagctaaaat ggtaatcaaa
     7861 ggaatcaaag cattcctgaa agcgtgtttc cagataatca cttgtctggg taagcccttg
     7921 gcctttgctg tacgaataaa gtcattccga agtgcttcta gcattgagga acgagtaaag
     7981 cgggagaagc gaccaatctg ttgcaggctt aaagataaaa caggaagcac caagtatttt
     8041 gagcggtcta aaatggcgcc ccagaatccg aattcagggc caccaatggt ttgtgtgccg
     8101 ccggctttaa ataagggaat ccatactgca aagacaataa tacagacgat ccctaaccag
     8161 aaggagggga tagaaattcc agcaaacgag aagaaattaa aaatataatc cattttgctt
     8221 cccggccgaa gagccgagat aatgcccaca ggaatcgcaa tcatcagtga aaagccaaga
     8281 gcgaccatgg aaagaataaa agtattcaac aaccggggtc ccaataaatc agtcaccggt
     8341 acacgataag tgcgactgta accgaggtct ccttgggcaa tttcagaaat ccaatgtcca
     8401 taacggacat aggaaggttg atccaagcca tagagttgtt ttaagcggat cacatcctcg
     8461 gcggtaattt taggatttga ggaggccatc agatcgacag gatcccccgg catcaaagtc
     8521 attaaataaa aacatacgta ggatagaatc gctatcacta agcaggtttg aatgagacgc
     8581 cgaaggatat atgtggtcat tgagttctcc tgattagttt aaagtggtac tgccaaggtc
     8641 ccatttttct gcttcattcg tttcagcgaa ttgatggcca ctcactttgt agttcttcaa
     8701 attcttcggg accacagagg tatctgaacg gtagtataaa ggaagaacgg gaacttcatc
     8761 tgtataaaac tttaagattt ggtgaaccat ttcggcgcgt ttttgcggat tgaactcaag
     8821 ttccaaatcc tccatcagct tgtcgctttt cttattggac caattcatga agttctgtcc
     8881 agaccagcca tttttatccg ttggaataga agtagagtga aaagtcgatt ttggactgtt
     8941 ttcaggattg gaaacccagg cgaacatcgc catggatcca aaattgcgtt ttctcatggt
     9001 ttcgccaaag aagacgcgag ctggttcatt tttagtaatg atttctatgc ccaattgttt
     9061 gtactggttt tgcatataaa cttgcaccag ttcgcgaact ttatttcctg ccgtggtcat
     9121 gaacactaaa gacaatcttt tgccgtcttt tgagcgaaat ccatcagtgc ccatcttcca
     9181 acccgcttca tctaataatt ttccggcttc gcgtttagaa tagggataaa gaacaatgtc
     9241 cttgggatct tttgtaaacc atgagtcttt aggggaaata ctatgaaccg ccacctgttg
     9301 tttgccatcg aagagcgcct ttacaaggtc ttcgcggttg agaccataaa ggagagcttt
     9361 acgaacttta atgtctttaa gaatagggtt gtcgagattc aagtccacat gttcataagt
     9421 tacagatgga gtgaaagtga cgtcataagg caaattctca gcttttactt ttttgctgaa
     9481 ggtgaccgct tcgtcaaaag aaagccccaa cgtagaaatc atatcaatgg tgcctgaacg
     9541 aagattggcc tccatggttc cggtattggg aatcaacttg aagataattt tttgaatttt
     9601 cggtgtggga ccatagaagt agggattggg aacgaaggta acatggtcgc ccaatttaac
     9661 ttcagaaatc acgtaaggcc cattgtaaag acctttcata cttggatttt tggagtagtt
     9721 cgaattcttt tcatagcctt ctttttcttt accgtgtttg ttgaatacgg actcttcgat
     9781 atgtttgggc agcggtgcga actgacctag ttgaaagaag tcccaacgtg ctttttcata
     9841 agtaaaagtg cattttttag gatttttagg atccgcttca attttttcaa ccagagtgta
     9901 agtttctttt tcaccaacgg aaaccgtggg ggctgcggcc acttttaaag ctaacgcaaa
     9961 atcctcacag atcactggtt ttccgtcgcc ccatttagcc gcgtcgatga tttcccagat
    10021 tgcaatgatt ttcttttttc cacccacatc gacgatttta gcgccgccat tctctaaaga
    10081 agggatcgac tttgcaagtt gtggatacca ctttccgttg ctatccaagc tcaccagagt
    10141 gcggtcagca aggctcagca tataagaggt ggctgccatc gtcttaatga gaggattgaa
    10201 attttcgaac tcttggctga taccaatttt gagctcagtg ttatttggag ctgaggttgc
    10261 tgacattgct atagtggcgc tgccgactat agacagtatg gccagtaagc tcagagtgaa
    10321 aatcgctttc atggagactc cttttttgaa gtttcaatat tcggttttaa actgggatcc
    10381 aaagttcgtg caaagctctt attttgctca accagccagc aggaagcctg gtgatcagag
    10441 cccttcaact gagggcgttc ctttgaacag atatccatca tataggcaca acgaggatga
    10501 aagtggcacc cggacggagg attgataggg ctagggacgt caccgaccag agaccttttc
    10561 atttgttttt tgccctcccc cacctgaggg atcgcaccta aaagagcctg ggtgtaagga
    10621 tgttgagggt tggcgaaaag ttcgtcccga gtggcgatct caacaatttt tcccaaatac
    10681 atgacggcca cacgatcaca gacgtgttcg atcactgaga gatcatgaga gatgaaaata
    10741 taagtgagtt ttaatttttc ttgaagatct ttcagcaggt tcaagatttg cgcttggatt
    10801 gaaacatcga gagcactcac gggctcgtca caaataatta attcaggatt cagagcgatc
    10861 gcgcgggcaa tgcaaatacg ctgacgttga ccgcctgaga attcgtgagg atagcgattg
    10921 atatgagagg ctttgagtcc caccagttct aaaagttctt tgactcgtat ttccgcttct
    10981 ttggaatcca aagttttatg aatttgaaag ggttggctga tgatttgtcc aatcgtcata
    11041 cgcggatcca gtgccgcata gggatcttga aaaatcattt gaatgttttt gcgtttttgg
    11101 cgaagctctt ggccagtcag tcccaagaag tctttccctt taaaagtcag atcgccgccg
    11161 gtaggctcat aaagacgaat caaggtgcgg cccaaagtgg attttccgca accactttct
    11221 cccactaatc ctagagtttc cccgcgtttg accgagagag agacatcatc aacagctttg
    11281 acttgcccta tttcccgcat cagaagaccc ttcttgatag ggaagtactt tttaatattg
    11341 cgagcttcga gaagaacttc ttgcaaggtg attctcctta gagcggattg aaacaagcca
    11401 cttcatggtc aggtccaatt ttggtcattg gtggtttatg agttagacat tcagatttgg
    11461 ctcgggggca acggttttga aaagggcacc ccgccggtaa gtccattggc gcaggaacac
    11521 tgccttcaat tgtctttaag cgattcgcgc gatgaccaag gcggggacgg gattcgatca
    11581 aagcggaggt atagggatga cggggattta aaaagagttc aagagtcgtg gctttctcgc
    11641 aggcctgtcc tccatacatc actaaaacac gatctgaaac ttcagagatg actcccaagt
    11701 catgagtgat gaattgtacg gtcatatgaa gtttatcttg caagctttgg aacaactcta
    11761 ggatttgcgc ttggattgtg acatccagag cggtggttgg ctcgtcagcg atcaagaaac
    11821 tagggttgca agaaagagcc atggcaatca tgactctttg gcgcatgccc cctgacaatt
    11881 gatgagggta ggttgcataa cgatccgcag gtgagggaat tcccaccagg ctgagcattt
    11941 caatagcgcg gtctttggca tctttactgg aaagcttcat gtgacgtttg atttgctcat
    12001 ccatctgata accgatggtt agaaccggat tcaaagcagt catgggctct tggaaaataa
    12061 tagccatttc gccgccgcgg acttcttcca tttctttttc tgagagagtc agaagatcgc
    12121 ggcctttgag tagcacctca ccgccagaga tttttcctgg cggttcaatc aaacgcatga
    12181 ttgaaaatga agtcaccgat ttgccacatc ctgattcgcc gacaataccc agggtttggc
    12241 ctttatgaat atcataggaa actttattta cggcgcgaat cggacccaat tttgatttaa
    12301 aagtggtttc taaattccgt acagacaaaa cagggtcagg caaggcaaac acaaatgcaa
    12361 tctcctctat gaatcagctg tgaatcaacc cgcgaaggcc attagaatga atatctcagg
    12421 gcacgatgca atgtaaagta aagagtttta ttgcagtgct acaggagagt ttttctctct
    12481 agatcttcat agacttttaa gagggcggaa gaggccgctc gtcctaaaat tccagaaagc
    12541 ttgtcggcaa gtttttgttt ttttgcgaag ctgactttga tgatttgatt ggtgtcagat
    12601 tgactcagta aataatcgtc actggtgcga atttcgtcca ctaagcccaa gtcaagtgca
    12661 tcttggccat accaatattc acccgtggcc actttgccga gattcacgct ggggcgattt
    12721 ttttgaacga agccttgaaa caagcgatga gtcgcttcaa gctgctcttt aaacttttct
    12781 tcccctttct ctgtgatctc acccaacaga ctgactgttc gcttgaactc gccagcggtg
    12841 tattccttat agtccacatc atatttcttg agaacacgat ggaaatttgg aacctgagcg
    12901 acaactccaa tcgaaccgat gatagcaaaa ggagaacaaa gaattttatt ggccgtgcaa
    12961 gacatcaaat agccaccgct tgcagcgact ttgtctacgc agacggtgag aggaatccct
    13021 ttttcgcgaa agcgaagcaa ttgagaagcg gccaagccgt aaccatggac gactccgccg
    13081 gggctttcca gacgaatgac cacttcatcc tgaggggtgg ccacattcat aacggcggtg
    13141 acttcttggc gaagatgttc aacagcagat gctttgatgt cgccgtcaaa gtccattaca
    13201 aagattttgc gttgatgttc tttctttttc tctttgagat cttttttctc tttttttgtt
    13261 tcctcttttc gctcttcttt gttgagggtg gcatttttga ggattttggc aaacattttg
    13321 tattttttgt ggagaagctc gatctcaatt tcagccttgt cctgggcctt ggcagccaac
    13381 aaagcaatta aaagcaagac tgaggcaatg gccagaacta taacgacagt ttgtgcagta
    13441 aacagcccga attgttgcaa aaaatccatt caacagactc cttgatattg tggaagtgcg
    13501 aatatagtca cgatgtgggt tgttataata ggacctgttt agatgaaggt cgagtatatt
    13561 ttcaggtttt ttctttggtt attctaaact tcaaaaagca gggtaaactg tcactatgtt
    13621 tttacgattt attatttttt ttcttatcag tgtttttata acccctcatt ctttggcgca
    13681 aagcgaagag gaaaggcgca aaggttttta tgaacaccag aaggaaaata aaaaatttga
    13741 gcaagaacgc gaagccggtc tgaaagacta tctgaaagaa caggccgagt gggaagaaca
    13801 acgtaaaaaa gatacattag ctgataaaaa aaataaaaag caagagactc ccgcagagaa
    13861 cagtgccgag taccgggcgg accgcaaagg aaaatacgaa gattacgaag agtacgaaaa
    13921 agcccgtaaa gcctatgtaa aagagaaaaa atctcatgag gcaaaaaatg ccaaagagca
    13981 agcaaagaga gaagaatggg cacttgaaga gtataatcta gataaagaaa gacctcgctt
    14041 tgatattgcg aaaagaaatc ttctgggtgg taaatcgggg ggaagtcctg gcggaggatc
    14101 gtcaggaggt ataggggggg gggcgcctaa ttttcctgcg cctccggcat ttgatgactt
    14161 tagcgatggc tatgtgccgc cgccctttcc gccgccggag tcttttgagc cttcaccgga
    14221 aagctttccg ccgccgccgc caattccttt tcctggcgaa gcagggccgg gaggagactt
    14281 cgatgggggc tactacccac ccccaccacc accgccatct atgccaatgc ctccgggagg
    14341 gggattctag ggaattattt ctgcacgaga agttcatctt ccttcaggcc acttaatctt
    14401 tgcatgcctt tagtcaggta taaaaagatc gcgactaaaa aaagaactga aggcaaaacg
    14461 atgattccca gggaccaagt ggtcatgtgg gcgatttgtt cattcagaat tgaagcctga
    14521 gcttcggcat ccagactttc attgataggt tcgaagatcc gcagagccaa aaaaaagtta
    14581 caaatagcgc taaatagaag agataaggaa atccacaacg tggccttctt aagaaggtga
    14641 tgaaattcgg tttcagtatt tctttccaat aaacgacttt tgagtttttc cacttcaatc
    14701 aattgagggt ttagaaagag agtggaaata aagggatctt tggtgaatgc agagccgaaa
    14761 acgaagctgc ccatcagtaa tggaaaagcc gcctctttca ctgcaaacca cattccatgg
    14821 agttttagga gagccagagt tccggtgatg ccgacgttta gaatccctaa gacggaaatc
    14881 gcattggttt tttttcgctt gatatgatcg taaattccat aagacagcgg aaaggacaag
    14941 gcgatgagca aagcccaaaa agatccaaga acctgactgc ccttattgag aatcaaaata
    15001 gggattataa tattaaacaa aatatttaga agtgagtttt caggtttttg ctgctgcttt
    15061 ttttccattg agccgtcctt taaaacatcc ttgagtgtcg aagattaaaa agcaagggaa
    15121 ccccacgaat ggtgcggctg gtcgtaggct gttatagagt gaggagggat ctctttcacc
    15181 tgaagtttct cagactaaaa aaataagcca aggggtctgg gattaagtcc gagatcgatt
    15241 ctcgacagag aaaatccttg ctcgactttg aaaaaactca tcaaataatt aagtcctaat
    15301 ttttaaagga aaagataata aaaccagata acaaggctag atgacaaacc tggataacag
    15361 atctcgatga taagcaggag acattttgaa tcacaaacaa cagtggaaaa tacataagtt
    15421 tggtggaacc agcgttctga attccgatcg ttataaaaat gtttttttga tcgtgagcag
    15481 tctggttcct tccagtcgca aagcggtggt ggtttcagcc atgaaaggcg tgaccgatga
    15541 cctcattaaa acagtcgaaa tggctcagca ccaacaggaa tcgtataaaa atctactgga
    15601 gagtatttat cagcggcact gtactgaaat caaggcgctt ttaggagccc ctgcgcgaga
    15661 cgctttgttg gccgtgatag aaagagattt tcatgaactc agagagattc ttcgaggagt
    15721 atggctagtt agaaccgcat cgaatcagat ttacgatttg gtttctggca tgggcgaagt
    15781 gtggtctgcg caaattttaa atgcctattt tcaattgcag ggggttaaaa cggattggtt
    15841 ggatgcccgt aagattttag tgattgaaaa aacagagcag gcggtcgtga tcaactggcg
    15901 ggcttctcaa gaaaacctca cacgttggca aaaggaacat gattatgatc tggtgaccat
    15961 cacgggtttt gtggcggcta ctgtggacgg gacgatcgcc actttggggc gcaacgggag
    16021 tgattattcg gcttcagttt ttggtgcatt gtttgattgt gacgagattt ttatatggac
    16081 cgatgtggat ggaatccatt cggcggatcc acggcaaatt ccagaggcca ccattctaaa
    16141 agaaatgtcg tataatgaag tcactgagct ggcttatttt ggggctaagg ttgtgcatcc
    16201 ggcgacgatg gtgccggcca ttaaaaagca aattcctatt tggatcaaaa acacttttaa
    16261 ccctacttgt ctggggacaa aaatttttca agacgctgaa tcttcggcgc cggtaaaagg
    16321 tttctcgacg attgataaac tcagcctttt gaatgtggaa gggacgggga tggtgggagt
    16381 tcctggcgtc gccgaaagac tttttggttc gcttcgttct tcagggatta gcgtcgtttt
    16441 aatttctcag gcgagttcag agcattctgt gtgtttcgct attcctgaag agcaaacaga
    16501 gaaagcgcag aaagccatcg agttggcgtt ttttgcggag attcaacagg gcctcattca
    16561 aaatgtgcag gtgacaagaa acgtgagtat catcgccgct gttggcgata atatggcctg
    16621 ttcaccggga actgccggcc ggtttttcac ggctttagga cgaagtggcg tgaacgtgcg
    16681 cgccattgct caagggtctt cggagcgaaa tatttcagcg gtgatcgata ctaaagaagc
    16741 gattcgagca ctgcgcacag tgcactcggc ttttattgtt ccccatcaga ctctttcggt
    16801 gggattgatt ggacctggtc tgatcggtcg cactttttta cggcagctta aagaaaaaca
    16861 actggatcta aaaaaaggcc gggaagtgga tgtcaaaatt agagcgatcg cgaattccaa
    16921 aatgatgtat ttatcggaaa aggaaattcc gttgaatcaa tgggaagagt tgtttgcaaa
    16981 aaatgctgtg cccttggatt ggaataaatt tactgagcat ttgaagccct cgcatcttcc
    17041 tcatgcagcg gtgattgaag ccaccgccag ctcttcctta acaccgcttt ataaggagtg
    17101 gttggcgcaa gggcttcaca ttatttcacc taacaagaaa gccaacaccg attccatgga
    17161 tgaatattta aaaatccgtg ccgcggccaa acgttatagc cgccattttc tttattcgac
    17221 caatgtgggg gccggtcttc ccattgttca gacgttgcgg gatctaatag cgaccgggga
    17281 tgagattaaa atagttcagg gcattttgag tggcacgctt tcttatattt ttaatcaata
    17341 cgatggcaca cagccttttt cggagattgt taaaaaagcc aaagaaatgg gttttaccga
    17401 gcccgatcct cgagaggatt tgtcaggtca agatgtcgtg cgaaagcttg tgattttagc
    17461 gcgggaaacg ggtatgaatc tcgaagtcga cgatgtggaa gtgcaaggct tggttccctc
    17521 ggctttaacc gccatctccg tcgatgagtt tatgagtcgc ttggccgaga tggatgctcc
    17581 tatgagtgag aagatgaaaa atgcgcaagc gcaaaaacaa attttgcgtt ttgtggcaac
    17641 gcttagtcaa gatggcaaag cccgcgtggg acttgaggct cttccggcgc agcatgtttt
    17701 ttccagagtt agtgggaccg ataatattgt cctctttaaa acaaaacgtt acttcgagca
    17761 acccctggtt attcaaggtc cgggggccgg tccagaagtc acagccgcag gagtttttgc
    17821 ggatcttctg cgactttctc agtacctcgg ggctctgccg tgaggactct tgccatgaag
    17881 aaagttgtca tgaagaaatc agtggcgcgg gcttttgctc ccgccacagt ggcgaacgtc
    17941 gctgtgggat ttgatatttt aggatttgct ctcagtggtc ttggagaagt ggcgactgtt
    18001 cgcaaaatca atttgccagg acaggtgaag gttcacccag ttgcggggtt tcccatgtta
    18061 cctgtcaatc ccttgaaaaa cacttgcagc gcgggcctgc ttcaactgct gaaagataag
    18121 gatcttcctt ttggttttga ggtctcgctt aaaaaatcca ttcccatcgg ctccggtctt
    18181 ggcggttctt cgctgtcagc ctgtgcaagt attgtcgccg cgaatgcact tttacaaaag
    18241 aagttgtcaa aagcggaagt cttgcattac gccctcatcg gcgaagcggt tgccagcggc
    18301 gccattcacg ctgataatgt cggcccttgc ctggaagggg ggcttatttt ggttcgccag
    18361 catcctgaga tctctttagt gaaagtcaaa actccttctt ctttacgatg tgtggtgatt
    18421 ttgccggagc tttccattag tacccaagaa gctcgaagtc ttctgactcc gcaagtgccg
    18481 ctcaaggcga tgattgagca aaccgccaat ctggctggtt ttcttattgg ttgcgtcgct
    18541 aaagatttta aattgatggg tgaatccttg agggatgtcg tgattgaacc gcaaagagca
    18601 aaattgattc cgaattttgc cgatttgcaa gcggccgcat tggatgcggg agctttggga
    18661 tgctcgatct ccggctctgg tcctgcggtt tttgcattag cgaagaatca aacttcagct
    18721 ttgaaaattc gcagagcctt gctggcagtg tcggcgaaaa agaaattgca attaagaggt
    18781 tcatggatct caccgatcgc aagtcaaggg gcgcacctat tatgaaatgg gtttcaactc
    18841 gtggagacag cactttattt tctacttctg aagccttgaa acagggactg gcgcaggatg
    18901 gaggcttgtt ggttccacaa gtcgatcctg aaattaaatt tgatcctagt tggggagagc
    18961 ttcccttttc gcagttcgcg gagaaagttt taagaccttt tttcgtaggt gatattttgg
    19021 aagaatcctt gccggcgatt tgtcaggagg cttttaattt tcctatccct cttgaagccc
    19081 tttcggaaga caccagtctg ttggaacttt ttcatgggcc caccagtgct tttaaggatg
    19141 tgggggcccg ctttttagcg ttgtgcctgg caaagattcc cgatgcccgg gacaaagtgg
    19201 tgttagtcgc cacttcgggg gatacgggtg gtgctgtggc cgcagcgttc tgtgaatgca
    19261 gtgatatacc tattgctgtt ttatatccga aaggaaaaat ctctcagcgt caggaaaagc
    19321 aacttacggt gtggggcaaa caagtgcaag cttttgccgt gcgtggcagt tttgatgatt
    19381 gccagagaat tgttaaagaa gcttttttat cgaaggcctg gcaaaaacgt ttccaaatga
    19441 tcagtgccaa tagcatcaat atcggtcgtt tgttgccgca gatggtgtac tttgcctttg
    19501 ccagtttgaa gtgtcagttg aaatctcagc tcaaaaacca cagcaagccg acgatcattg
    19561 tcccttctgg aaatcttggt aatggggtcg gcgcattgtg ggcgctgcgt ttgggatttc
    19621 ctatccagca ggtgatcttt gcacataatg ccaatcaagc ggttcctcaa tattttgcgg
    19681 aaggggcgtg gtctcctcag tcgacgattg caacgcttgc caacgccatg gatgtcggga
    19741 acccttctaa ctttgaacgc cttcgctatt tttacccgaa cttgaacgca ctaaggaaag
    19801 tggcttccgc gcactcagta agcgatgctc agattcgtca aaccatccaa gagcaagctt
    19861 ccaagggatg gattctttgc cctcatacgg caacagccgt ttacgtccgc gagctgtaca
    19921 aagaacgctc ttgcatcgtc gtggccactg cccatcccgc taagtttgaa acaattattg
    19981 aacccttgat tgggaagacc atttcgattc cacaaaattt aaatttgtta ttgtccaagc
    20041 catccgtatt taaagaaatc agtccagatt tagcgagcct ccatattatt atttgaagat
    20101 aaagtgtgat aagcaatttt atgttatgga gtgcaaaatc gttgaaaagc ccgtcgagga
    20161 agttattagt ggacgggctt ctttttataa ggcaattcct cttccttggg ttgattccag
    20221 acttgaaaat aaatcatcgt ccaaaataag accccggagg tcaggacggg gatcaaaaag
    20281 gccgcatgac caaaacttga aaaggcaaag cctccaatga cggaaccaaa gccaaagaaa
    20341 aaaataattc cgatcctcat cagggtggct tttttctcgt tgccaatatg atcggccaac
    20401 ttgcgctgat tcaagaggcg gacaagacct attcccaggt ctgtggttat tccagtcaga
    20461 tgcgaggtgc gaataacgga tttggaaact gttgtaatgg taccattctg aatcccacat
    20521 ataaaacaaa gtaaaatcaa taaagtgtag ttctctgagg tcgtcaaaga ttcgccaaat
    20581 tggcctaagc ggccaagaac tccagcgatg gtcacaaata gagtcaggaa aaaaatgact
    20641 ccgaaggtca gatagtatcg gggcttttta tggagtttaa ggcgaaggtc taccaactgt
    20701 ccactgacca ttgcgccgag aagaaaaaat aacggaacca ctagcatccc taaagcatgc
    20761 cgaggctggc cctggcttac gtcgtacccg aaaaaagtgg caaagcctgt cacatgtgaa
    20821 acgaagcgct ggcaggccat aaaaccacca atattcaaca caccggcttg aaaggccata
    20881 accatccaga tagagatatt actttttgta tagtgagaga tagattcgtt accgtagagc
    20941 atatccagat cccttaataa ctccaaagag agccatggct ttggtttaat tgaatcttaa
    21001 cttaaccccg gcattgaagg caacggcttg agtttgcacc tagactcatc ttaagcttaa
    21061 gtttaaacct aagatgaggg ctaagttttg caatccagag ttcaagctaa gagttcaaat
    21121 taagagtttc cattaagatt ttcaattctc gttttcgtca gttccagttc ttttagaagt
    21181 tccaacttct ttttttgact gttaaaccat cgttctgtgg ctcctaccaa atctggcgaa
    21241 tttttaatgc ctcttttgta ttcactcacg gtcatttcaa aatatttttt agaggtgacc
    21301 agtttcgctt cgttgatctc ataaagatca ctcaactcct ggagtttttc ttttaaggaa
    21361 ttgaaactac tctgggagct aagaaccgct tgagttgccc tggatttctt agctaaggtc
    21421 tcatattttc cagcagtgtt tttgtgaatc gtgctaaaac cggaaaataa aggaatactt
    21481 acctggattc catattttga ttcattcatt tttactgggg aagtttcaga gggggtcaat
    21541 cggccaaagc tatagacgaa atccaccgag ggcatgaagt cggctcttaa ctctttttgc
    21601 tccaaatgag aaagtattag cacaagttgc gctctttgca cttcaatatt attttgttca
    21661 ttaaaagact ttacgtatct taatttttca atactgtcaa aagtcatttt atcaagttca
    21721 gtatcaggaa tatcttggcc aaataattca aagagctttg catgagattc cctgtgtaat
    21781 tgatcaattt gcttttgctg gatttcaatt tctttttccc gcaaatcaag ttcgagatta
    21841 tccacagagc ttgtgagccc agaacttact tttttggccg ccatcatttt ttggtctttg
    21901 gtgatccggg cttcatcatt gagaatcgtc tgtagtttgt ggagataaat catattgccc
    21961 atggtctcag ccagcgccaa gcgaatttct agacgagaat tttcatactc aagttttttg
    22021 agttgaactt cgatctcctt ttggtccgaa atagcaatgt ctttaagccc gttaaacaaa
    22081 ttgagacggc cttctaggta tccaaaataa ccctgattgc tttcttccgg gttgtccatg
    22141 cggttttgac cccatcccgc aaccgcacta aggctcggat agaggggaga gtaagaggat
    22201 tgtaaaagac tttcttttga ttggagctct ttttttaagg cggacagagt ttgattctgc
    22261 tcgttcagtt gctgctcaag agaaaaaaat ccctcaatgg cgtgagcctc catgctcaaa
    22321 gcgaaaatta aagaaaagct cgcgggccag cttacgggcc aacttactgg ccgacttaca
    22381 gaccagctca tcaaagaaag tctttttttc atagtcaaat ctccgcaact gaaactattt
    22441 ttccacttgg aatttgaagg agtccttttt gccgttggtt tcggtgttca cgttgacctc
    22501 aactcgatag gcatttttaa agtccacttg ggttgtcagt gccccctctt ttaattccac
    22561 ttttagattt tcacttttgc ctctcggggc tttcgccgtc gcttttactt tgatttgaga
    22621 aggttcaaga tcttttccct catgggagag gggatagagc ttcacttgat tggaagtgac
    22681 aatatattcc aggttgatct cttttcccgc cagcacgctg cccccgtgaa gcgctttcag
    22741 tgccccaggc gcgacgtcat gaccttcatg cgcaagagct ggttgtagaa ataaaatcgc
    22801 agacattgag agtaatattt ttttcatagg atcctctttt ttaagtttta tccctgctta
    22861 gtggggagtg ggttttagtt cagtttctat tgttttaata tttttttcgg cggatttttt
    22921 accaaaccga taaaacacag tcggggtaac aaacatatcc aaaagtgtgg agctaatcaa
    22981 tcctccgacg atgacgacag cgactggatg cagaatctct tttccggcat ctcctttagc
    23041 taagacgagg ggtaacagcc ctaagatggc cgtgagggta gtcatcagga caggaacaag
    23101 tctttctaag gagccgcgga taaccatgtc tttggtgaac acctcgccct catgtttcat
    23161 taaatgcaag taatgcgaaa tcatcatgat tccatttcgg ctcgcgatac cgcaaagagt
    23221 gacgaatgcg accatagtgg caatagagaa agagcgattt gaaagataaa tggcgagaag
    23281 gctgccaata agggccatgg gaatattaag cataatttgc acggcgatga aaaaactttt
    23341 gaaatggaag aacaggacaa tgaagactcc taacaaggag atgattccta aaaataaaat
    23401 cagacgactg gccaaacgtt ggctttcgaa ctgaccgcca tattcaataa aatacccttc
    23461 tggcagcgaa actttttgtt tgattcgttg ttggatctcc agcactaagc tgtctaaatc
    23521 gcggcctgaa gagttcgcgg aaacgaagat ccggcgttgc gcattttccc gattgatttg
    23581 gttaggaccc tgggattcat aaacatccgc gactttttct agagtgactt tggttccgtc
    23641 cggcatgatt tttagtgctg tttttttcat gacttcaagg tttgctcgag agtgatcatc
    23701 gaaacgcatg taaacattga aggttttttg ttgatccaga atttcactga tgacttcacc
    23761 tttaagagct ttctccaaag tttcagagat ctcgcccagg atcattccat attttgcggc
    23821 ttcggtgcgt aagagttgta ttttcacttg cgggattaag acctgttgct ctacctgtag
    23881 atccaccagg cccgggatcc cttccaaggc tttgaaggca tctgaggcct tggttctaag
    23941 gatgctgagg tcatggccaa aaatcttaat cgccacctgc gctcgcactc ccgaaagaag
    24001 gtggtccaca cgatgggaaa tgggctgtcc tatatttacg aagatatctg gaatctgttt
    24061 caatttgtcg cgcatctcgt ttagcacggt ttcgcgagat cgtcctttat ctttgaagtc
    24121 gacatcaatt tctgaagaat gaactccttc ggcatgttca tccaattccg ctcgacctgt
    24181 tctgcgcgca agtgatttta tttctggaat cgacaagagt gcccgctcga cttgggtgcc
    24241 aagatgattt gattcctcta aagaaatccc tggttgagcc agtacactca ccgtggccgt
    24301 gccttcattg aatttgggca agaagtcttt ccccatcagc ggaatcagca gtaaagaggc
    24361 taagaacaaa gccaatgtgg cgcctatgat tatttgggga tgatccaggg cttttgtaag
    24421 gatctggcga tcccactgtt taagtttttt aacgagcttg ctgtctccgt gctctcggag
    24481 tgcgtctttt gaaagcagca gagagcacag ggccggagtc accgttaacg agacaaagag
    24541 tgaagatatt aaagaaatga tatacgccaa tcccaagggt ctaaagaggc ggccttcaat
    24601 tccactcatg gagaacaagg gaagaaaaac caagacgacg atgactgttg caaagacgat
    24661 ggaattccgt acctcagagg aggcgttgta aacgacttgt aaagtgggca ggggggctgg
    24721 taactgtcta ttctcacgca gcctacgaaa gacattttca acgtccacaa tagcatcgtc
    24781 cacgagctca ccaatggcga tcgccaatcc gcctaaagtc atggtgttaa cggaaagtcc
    24841 aaaaaaatga aacacaatgg ctgtgattag aaaagaaagg ggaatggcag tgagagtgat
    24901 aagagttgtt ttgaaattca tcaaaaagaa aagcagcaca ataaagacca ggatgctccc
    24961 gtctcgcaga gcttctttga cgttgtcagt ggcggcttcg ataaaatgag cttgtttgaa
    25021 aagctctttg gttaactgaa ctcctggggg aagggagctt tccagatctt tgagagcgac
    25081 atcaatggtt ttggttaatt caatggtatt ggtgccaggg tgtttttgaa cgctaaggat
    25141 caccgcaggt ctgccgttga cgctgccatc tcctcgtttg ctttgcgcgc cgattttaac
    25201 ttcagcgatg tctttgacga aaacaggttt gcctaaatga agtccgacca cggtattttg
    25261 gatatcttct tcggagcgaa ccactccaat gttacgaatt aaaaattctt ttttgtcgat
    25321 atcaatatag ccccccgttg tattgaggct gatgttggac aaggcgtgtt cgatgtcggc
    25381 gagtggaatt tgcgattttt gaattttctc tgcggagaga agaatttgat attgtttgac
    25441 cccgccgccg atggcgatta cttgagcaac gccgggaatg gccaataagc ggggacggag
    25501 ggtccaatcc gcataggtgc ggagatccaa gggatttact ttggtatcgg gagaagtctc
    25561 cgctatggaa agaccaatca gttgaatttc ccccataatg gaagagatag gtcccatgat
    25621 cggcgtcgct cctcgtggaa gtttctcttt ggctaactga agtttttcag agaccagttg
    25681 gcgatttctg taaatatccg tatcccaatt aaactccaca taaatcaccg agagcccaat
    25741 gcccgaggtt gagcgcactc gctccacccc tggaagaccg ttaagaacgg tttccaaggg
    25801 cagggtgact aaagtctcca cttcttccgg cgccatcccc ggggcttcag tcatgatatt
    25861 cacagtggga cgattgagat ccggaaagac atccacaggc atgcgactca aggtaaaaag
    25921 accgtaagca gtgaggacag cagcaagaac caggatccat aatctatggg tcagcgaaaa
    25981 gcgaataaca cgatctaaca tgagtcggct ccagattgaa ggtgagtgaa agtcagtggt
    26041 ggctgccggg gctctcgctt gtattctgta atttcaaagg caatggataa gtgacgtcgt
    26101 tgcctttttg cttattgtgc tgactggtaa cgatcagctt tccatttttg gttaagtccg
    26161 cgtctttggg gaagatgcat tcaaagaagc ttttgtcttg ggtcttttgg ggctcacaag
    26221 cggactttaa cgtctttttt cctttgtaaa tgatttcaac actggagtcg ttgagtcctt
    26281 gagtttgcca agaaatatcg agaaaaaaaa cttttaattt atgggttcca tctgggacga
    26341 gttcggtgtg gtaagcgccc ggcatttgta taaagccccc gttggggcct agtttatctt
    26401 cgccatgagc aaaggcattt aaagagaaga gtattgtcac tgcgcaaatg agatgtttca
    26461 tataataaat cctcgaaata ttaagaagta atgatccttt catactacga gacaaggagg
    26521 caatttgtga caaaaaaact gaaaatcttt ttgcgttttg gatgatgtta taaccctatg
    26581 atatgattga tcattcgggc ggacacttta agcttgaata ttaacttaac agagcggtga
    26641 acttcagtta aaccttttgg gcatgagtct aaaaatctcc agaattcttc acgccgggta
    26701 tgtgttcgag tgtgacgaaa ctcaaattgc gtttgatcct atttttgaaa atccattcag
    26761 tcgcaactgt tatgctttcc ccagtgttcg gtttgatcat gagcaaataa caaaagttaa
    26821 atttgcggcc gtttttattt ctcattttca tgacgatcac tgttctttgg aaagtttggt
    26881 tctcttagac cgaaaaacac cgatttatat ctattgtatt tttgaggaac tcttttcttt
    26941 aatcagagca ttgggcttta cgcatgtata ttcattggag atgaacgttc cggttgtcat
    27001 aggttccctt gaaatcattc cttggagagc tttggatgcg gacgtagatt ctatttttca
    27061 aatcaaagcc gctggtttga atgttctgaa tgtcgttgat tcgtggatcg attttgcaac
    27121 actgagtgaa cttgcgcgaa gcgcgccctg ggacatggtt ttgtggccgt tccaaactat
    27181 gcgggaaatt gaagttttgg ctccttctcg agccactgtg gccacaggtg agttgccgac
    27241 ggaatggata gagcaactca aaatattgaa tcctaaatat gtggtaccga gttcctgtca
    27301 attcattcaa gaaagctggt cttggtacaa tcacgcgttt tttccgattt cttatcggca
    27361 gtttcagaaa gaaatcgaag acgcattgcc tgattcacag gtggttagga tgaatccgtc
    27421 tgtcgccgtg gttctggata agcacatgct tgcg
//
LOCUS       DATBTE010000408         2569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_125058, whole genome shotgun sequence.
ACCESSION   DATBTE010000408 DATBTE010000000
VERSION     DATBTE010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125058"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="VIG33_15685"
     CDS             complement(<1..129)
                     /locus_tag="VIG33_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEY8272333.1"
                     /translation="MVPIVIEQTARGERSFDIYSRLLKERVIFLVGQVEDYMANLVV"
     gene            complement(193..1491)
                     /gene="tig"
                     /locus_tag="VIG33_15690"
     CDS             complement(193..1491)
                     /gene="tig"
                     /locus_tag="VIG33_15690"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY8272334.1"
                     /translation="MQVSVEKTSELSRKMTVSVPEEVVQEKMAARLKSLARSVKIDGF
                     RPGKVPQHVVEKMYGDRVRGEIAGDLIQSTYYDALQDQNLRPAGHPHIHPSDEAEGFK
                     YTAEFEVYPEISLEGVEEIEVSRPIATVQDADVDGMIEKLRAQKKTWNVVERESREND
                     RVTISFSGTSEGENFTDGKVENYQVEIGAKQMIPGFEENLIGLKAGDNKTFEVSFPEE
                     YGNEKLAGKAAVFEVEVTSVEEPVLPEIDEAFINAYGVEDGSVDSFRADIKNNMEREL
                     EQALRGKLKNAVMDALYEKIQITVPNTLVDQEIESMMKPYIETAKRQKMKLEDLKLPR
                     DAFEEQAKRRVALGLILGEIIHKNEIKIDSNKVRSTIEDMAKSYERPEDVVNWYYSDE
                     SRLNDVQQMVLEDQTIEWLVARAKVSDETVNFNDVMAKQR"
     gene            complement(1600..1684)
                     /locus_tag="VIG33_15695"
     tRNA            complement(1600..1684)
                     /locus_tag="VIG33_15695"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1648..1650),aa:Leu,seq:tag)
     gene            1890..2141
                     /locus_tag="VIG33_15700"
     CDS             1890..2141
                     /locus_tag="VIG33_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272335.1"
                     /translation="MNITPTASAVNLINTAQHKSADAAQVIARLPIQKDEVGSSEFKS
                     SDIIKPVLSLKEAELETSAAVKLLEADKETIGSLLNTKA"
     gene            complement(2143..>2569)
                     /locus_tag="VIG33_15705"
     CDS             complement(2143..>2569)
                     /locus_tag="VIG33_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272336.1"
                     /translation="LVHRVGVQNGLKKLKEYGGEVWFKLDSATEEGRALMNNAGQSCQ
                     ASVENLLLCAKLCPTKLQTCLVDYDKQGSSQKEKNAYLDLLRSIKTKGCALKGVMLYT
                     IARPSLQPESGRLERLPVEVLNEFADEIRLLGFDVWVSG"
BASE COUNT          731 a          673 c          404 g          761 t
ORIGIN      
        1 aacaaccaga ttagccatgt aatcttcaac ctgccccacc aaaaagatga cccgttcctt
       61 taataatctt gaataaatat cgaaagaacg ctcacctcga gccgtctgct caataacgat
      121 aggtaccaaa ccacctgctg cttctggagc catcatttga tttcttatat attggttata
      181 cattcaaacc ccttaccgtt gcttggccat cacatcgtta aaatttacag tttcatcaga
      241 cacttttgct ctcgctacca accattcgat tgtctgatct tccaaaacca tctgttgcac
      301 gtcattcaag cgactttcat ctgagtagta ccaattcaca acgtcttcag gacgctcata
      361 acttttcgcc atatcttcaa tagtagagcg tactttatta ctgtcaattt taatctcatt
      421 tttatgaatg atttcaccca aaatcaaacc caaggccact cggcgcttag cctgctcttc
      481 aaaggcatct cttggcagtt ttagatcttc cagtttcatt ttctgccttt tagcagtttc
      541 gatgtacggt ttcatcatac tttcgatttc ctggtcgacc agtgtatttg gaacagtgat
      601 ctggattttt tcatataaag cgtccatcac tgcgttttta agcttaccgc gcaatgcctg
      661 ctcaagctct ctttccatat tatttttaat atctgcacgg aaagaatcca cgcttccatc
      721 ttcaacacca tacgcattaa taaaagcttc atcaatttca ggcagcacag gctcttcaac
      781 actagtaact tcaacttcaa atacagcggc tttaccagcc aacttttcat tgccatattc
      841 ttctggaaaa gacacttcaa aggttttatt gtcgccagct ttcaaaccga tcagattttc
      901 ttcaaaaccc ggaatcattt gtttcgcgcc aatttcaacc tgataattct ctacttttcc
      961 gtccgtaaag ttctcgcctt cggacgttcc tgaaaagcta atcgtcacgc gatcattttc
     1021 ccgggactca cgttcaacaa cgttccaggt tttcttttga gcccttagtt tttcaatcat
     1081 gccgtcaaca tcagcatctt gaacggtagc aataggccgc gacacctcaa tttcctcaac
     1141 accttccagc gatatttcag gatagacctc aaactcagcg gtatatttaa agccttcggc
     1201 ttcatctgac gggtgaatat gcggatgacc ggcaggcctt aaattctgat cttgcaacgc
     1261 atcgtaatag gtagactgaa tcagatcacc ggcaatttcg ccgcgcactc tgtctccata
     1321 cattttttca actacatgct gaggcacttt acctggacga aaaccatcga tttttactga
     1381 gcgcgctaac gactttaaac gtgccgccat tttttcctga acgacctctt caggcacact
     1441 aacagtcatt tttctgctta attctgatgt cttttcgaca gaaacttgca tttctttacc
     1501 tcaactaaat ttaaataaga atttgataga ataccggtaa acggctcaaa agaactgaag
     1561 agttaacgat ggctcactaa ctcttaatta tgaattgggt ggtgcgaatg gagagactcg
     1621 aactctcaca ggataaccta ctggaaccta aatccagcgc gtctaccaat tccgccacat
     1681 tcgcacttta aatgaactcg taattatatc cataattata cgaccatcac aagctataaa
     1741 tttaaaaatc atatgcctgt agttgttcat tgtttaagaa aatgctttgt cattcacgct
     1801 tctatcctaa aaccacaatc caaactttgc ataccatctt tatcaatgta gtattagttc
     1861 gacgccaatt caatgtataa gggcatgaaa tgaacatcac accaactgcc agcgcagtta
     1921 acctaatcaa taccgctcaa cataaatcag cggatgccgc tcaagtaatc gctaggctgc
     1981 ccatccaaaa agatgaagtc ggcagttcag agttcaaatc cagtgacatc atcaaaccag
     2041 tacttagcct aaaagaagcc gaacttgaaa cctctgccgc tgttaaatta cttgaagctg
     2101 ataaagaaac aattggatct ttgctgaaca ccaaagccta gactaaccac ttacccacac
     2161 atcaaagccc aagagcctga tttcatcggc aaattcattc agcacctcca ccggcaacct
     2221 ctctagccgg ccggattccg gctgaagaga aggtctcgca atcgtataca gcatcacgcc
     2281 tttcaacgca cagccttttg ttttaatact cctcaacaaa tccaaatagg cgtttttttc
     2341 tttttgagac gagccttgtt tatcataatc cactaaacag gtttgcagtt ttgtcggaca
     2401 aagcttggca caaagcaaca ggttctctac actcgcctgg caactctgcc ctgcattatt
     2461 cattaaagct cttccttctt ccgtagcgct atccaactta aaccatactt ctccgccata
     2521 ttcctttaat tttttcaggc cattctgaac gccaacccga tgcaccaaa
//
LOCUS       DATBTE010000409        27330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1831959, whole genome shotgun sequence.
ACCESSION   DATBTE010000409 DATBTE010000000
VERSION     DATBTE010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 27330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27330
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1831959"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1914)
                     /locus_tag="VIG33_15710"
     CDS             complement(40..1914)
                     /locus_tag="VIG33_15710"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY8272337.1"
                     /translation="MDNKEPMTFLRIRKYFSVKKSLIPFFALGMGVQIQNGQNSFLQT
                     SPSSEEIASDILQEHREVSPSDSLKSLPGLVVLGSEVTTYTGSLFLRGANGDQTLVLW
                     NDFRSDNFTGPSGATDPFGLGSEFSNRIRVLKGPQSLLYGTQAMGGVVLIENDSDVDS
                     SIELAGGSLKTSRGLGEFRFRGEKWQLALGGSAFSTEGVSAYNASVARGTEGKLESDG
                     RQKDSLSLIASMDFSAQDQLQIMVNGLQDSLNDDAPPQEDINAHSEARTKQWKIRYKT
                     LWSERVGSSFLLTSQETKRENKNPADIYNPDEYMDTSYGQRISILNRNNIRWLKSLWQ
                     VGFEYNEEQGDFFSSTNSAFVNPQTFAFNPKTNDESVYLVNDWNFKSSDISWGLRENC
                     RESKACLTVYQLAYQWHWVESQRSVYGLLSSGLKRPVLYQLYSRYGDPLLRAEHSQAY
                     EVGMIQRWGLTQEFKLSVFDNHFSDLIDYDFITEKYKNLKRVKTQGVEIKHQYEGVTW
                     DTQFSIAQIYAKDEDSGLYLLRRPVIQGAWSLGYQIFEPLHFMNQFIYVGSREDVDIA
                     SRVVLKEATLWNVALNYKSGVYGHYFLRINNLGNIFYEDIKGYLTPGRFVWLGTQLLF
                     "
     gene            2033..2743
                     /locus_tag="VIG33_15715"
     CDS             2033..2743
                     /locus_tag="VIG33_15715"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY8272338.1"
                     /translation="MRHQRSSRSEKSEQGKGKHQDRSQDRMQGQQREDESLSRWQDRD
                     EDKDYQDQGYRAEDYSRFSRSDRNMNMETNQRPGWGREFSGEETSYGGGRGYYDEGSY
                     GSQQGVSSQRGSRSEAQGRYAGRGPKGYTRSDERIKEEISEALTRDHEVDASDIEIEV
                     KDGEVTLTGFVPERKMKHSAEDLIEKCMGVKDITNNLRIQKEEGRGEAESERGEKRGA
                     GSQASKKSGSTSTSSLSH"
     gene            complement(2884..3297)
                     /locus_tag="VIG33_15720"
     CDS             complement(2884..3297)
                     /locus_tag="VIG33_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272339.1"
                     /translation="MKKFSAIIVLLVTSSTVHARWTKYMDFGGNEDQYYTNPLNEKQC
                     SLHIAHEVNEDGTRDAYVEFKQHDLYSASTLASNFKLDIKGEVRNDGASDFTAQSLPT
                     LTGSSLSAEVKGHSMRFAVKRPVGTPLNCYWNYQN"
     gene            3689..4441
                     /locus_tag="VIG33_15725"
     CDS             3689..4441
                     /locus_tag="VIG33_15725"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEY8272340.1"
                     /translation="MCKIFFLYILFTLSFRAQALQVMIDPGHGGLDSGTSHLGVQEKD
                     LVLKVSSYLKTLLEENPQFNVEMTRETDQQLSLRTRVKKAEKAQSDLFISLHANSNPD
                     MRIKGTELYFQNSLPPDEESLILAEQENQAETTAAEAKTALQDSPSKKGDIRAIIEDL
                     HRQYRAKSSLKITQILAADWSHSAIKQAPFYVVSKTSMPSVLIEVGFLTHPEEAKKLN
                     TPKYQQEIAQKIYKAVLQYKEMMDKIEGQALE"
     gene            4466..5206
                     /gene="rph"
                     /locus_tag="VIG33_15730"
     CDS             4466..5206
                     /gene="rph"
                     /locus_tag="VIG33_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY8272341.1"
                     /translation="MRAHGRLPHHLRSVKITPHVSEYAEGSALVEFGRTRVLCTASYE
                     SKAPQWLMGSGEGWVTAEYGMLPRSTHQRMKRDKTASGGRTQEISRLIGRSLRAAVDL
                     KGLGEKQIMIDCDVINADGGTRTAAITGGFVALALALKKLHGLSEIKSIPLISYVSAV
                     SVGIQGSTILLDLDYDEDSSIGTDMNFVMTDKGHFVEVQGTAEDVPFTREQLNAMMDV
                     AQQGCFELFKVQEEIVGPIFKRTQISGL"
     gene            5221..5820
                     /gene="rdgB"
                     /locus_tag="VIG33_15735"
     CDS             5221..5820
                     /gene="rdgB"
                     /locus_tag="VIG33_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY8272342.1"
                     /translation="MELWLATGNKGKVNEYKLGLKELVDLKVFTQADIPSFTPRPEDG
                     KTFLENAHIKARTLKSVKPGVWVLGEDSGLEVEGLNNLPGIHSARYAGPKASDSENVA
                     KILKMMSIRPIANRNARFVCSTVIFTPGGEEWHFEGIMKGTISSKPAGQMGFGYDPIF
                     LPEGQTQTLAELGPSFKNQFSHRAQALKAFLQKLKSSES"
     gene            5991..6935
                     /locus_tag="VIG33_15740"
     CDS             5991..6935
                     /locus_tag="VIG33_15740"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY8272343.1"
                     /translation="MKGLKIFAGNSHPHLARKVAEAAGVDLGFSQVSSFADGEIQIEI
                     HESVRGQNVFVIQSTCPPVNQSYMELFIMVDALKRASAAQITAVIPYFGYARQDRKVA
                     PRAPISAKLMADLLTTAGVDRVVSVDLHATQIQGFFDGPVDHLFAIPTLARAWREQLG
                     IGPEFVAVSPDAGGVERTRAFSKRIESSLAIIDKRRSGPNEAKALHLIGDVTNKTAVI
                     IDDMIDTAGTLTQGVDSLIKNGAKRVFAVATHPVLSGPAIARIKESPIEKVWVTDTVP
                     LSEAAKACGKFEVVSVAPLLAEAIKRIHGNDSVSSLFD"
     gene            7042..7737
                     /locus_tag="VIG33_15745"
     CDS             7042..7737
                     /locus_tag="VIG33_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEY8272344.1"
                     /translation="MKQRIELTVETREVGKSHSRALRVANKVPGVIYGAIENTNVFVD
                     ARDVNKYNTRAYENALFNVKSTIDKANGKVVLIKSVDVHPLTRRPVHIDFFALDLTKT
                     VRVYVEIKLEGKPIGLAEGGLLNIVHRQIEVECLPTEIPEFITADVSNLGVGDALHVS
                     DLSVPGSVKVMTRPEETIAVVAAQEEEAAAVPAAAAAAPAATAAAVPAAGAKPAAGAK
                     PAAGAKPAAPAKK"
     gene            7804..8367
                     /gene="pth"
                     /locus_tag="VIG33_15750"
     CDS             7804..8367
                     /gene="pth"
                     /locus_tag="VIG33_15750"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY8272345.1"
                     /translation="MWLIVGLGNPGNQYSLTRHNIGFMAVDYHLKGLGDPPCKNQFKA
                     EIFQSKIGEQPVIFCKPQTFMNLSGESVQPLLGFYKIPLDHLIVVHDEIDQAFGQIKI
                     QKNRGHGGHNGIKSISSLLGSTDYIRLRLGVGRPAIPTVPIADYVLNKFTAEEFNALP
                     SFLNKAGDAIEAILRDGVQKASTSFNA"
     gene            8400..9500
                     /gene="ychF"
                     /locus_tag="VIG33_15755"
     CDS             8400..9500
                     /gene="ychF"
                     /locus_tag="VIG33_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEY8272346.1"
                     /translation="MALQVGIVGLPNVGKSTLFNALTSAKAEAANYPFCTIDPNVGVV
                     TVPDPRMEKITAFVKPQSVVPTVMEFVDIAGIVKGASQGEGLGNQFLSHIRQTDAIVH
                     VVRCFDDPNIIHVAGNVDPIRDMEIINTELMLADLDSAEKRVKRVEKMAKSTTDKKIK
                     NEFEVAKKVVEALSKGLPARSVGVDENEAPFLKEMHLLTSKPVLYAMNVSDTDFAAGG
                     NDWTRAVEKRAAEEGNTTIMICSAMEAEISQLPLEERKDFLESLGAEEPGLNRLIREA
                     YKLLGLHTYFTAGVKEVRAWTIRAGTKAPQAAGVIHTDFEKGFIRAETYHCEDLFNLK
                     SEQAVKDAGKYRVEGKEYVVKDGDILFFRFNV"
     gene            complement(9540..10430)
                     /locus_tag="VIG33_15760"
     CDS             complement(9540..10430)
                     /locus_tag="VIG33_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR2 nuclease fold domain-containing protein"
                     /protein_id="HEY8272347.1"
                     /translation="MTKVKVSAKTSAKNRTKSRAPKKEIKEKEYIVIDEAQGLIFENE
                     TDLFGYFKESIDALEDEYQSVRSEEDFSDEKQLELESYLESTLDEPDEIWQDTQTFPE
                     LPIHHFIRSFEVGDQVFHYIAVAYVTAEESYPSFVFIHFPTKDLRVLQNYQRGELIYD
                     KKYEYVQTGAIEGDALGEGDPLSMGLYIAMMKVRGDKDIPQELFQDFADLREETIESA
                     DEIWRKNDLEGNVLVTFIKEYPDHETKDLTYIVVTQEDSTTNVHSLLFSFPTNDSTLA
                     DRYRQGENLHAEEVVQESSH"
     gene            10622..10939
                     /locus_tag="VIG33_15765"
     CDS             10622..10939
                     /locus_tag="VIG33_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272348.1"
                     /translation="MSIIEEKSLERMEALLSHSAMGVHVLFDNKSIANVLSQVKDDRD
                     FYDFNKMKKVQDVMTELIAKRTYIEKISYLKSLEPEAYEMVVRAYFHIVENTARANQD
                     LAH"
     gene            11024..11317
                     /locus_tag="VIG33_15770"
     CDS             11024..11317
                     /locus_tag="VIG33_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272349.1"
                     /translation="MKSLGSFILILFGLSVITHAEYRMFTLKITNKNTQDYRLVDSTL
                     DPVQYPYYYPVQKDEVVQYTDTWRCYGRTGGGLPPCPNLKSNPATNPAPVANP"
     gene            11402..12697
                     /locus_tag="VIG33_15775"
     CDS             11402..12697
                     /locus_tag="VIG33_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY8272350.1"
                     /translation="MPIESSRTRRTSQNDLSDLQSEYAQKKKQYSHQQEEELNELKDF
                     YNDRKEQTRSQGEAAVNHIRRSQTESLNASNEARKKIAERSQAQISGIETDYRKKITD
                     TQAKRQADLDTARMNSHEKLNEIQKSQHDKVEQVRSESTKEIQTTKERFNQELAHTQQ
                     FTEKRISDIKGNNEKNMRQEVEKGRIQHTKLHENLQKDYDKVNSQGQQALAERREIQE
                     KQYQRQDGDYDKRFHKQHEQWDAREKNMNEEYNKRITHGKKAYETQLKDQHERFDSVY
                     RKNDMAHKESLKIQNNNLLKEQVELKKKFFKEKERYAGKEDDPFYKLQDRGNRLRENS
                     DFYIIEAFVPQHEKDNIKVVIKDDTAAISGKRAFIDKIEEEGRKLSTNSYQSFREEFN
                     FDKPVITEGMTRERNGDYVTFWVPKLTSFDGSRKLSKKV"
     gene            complement(12694..13491)
                     /locus_tag="VIG33_15780"
     CDS             complement(12694..13491)
                     /locus_tag="VIG33_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY8272351.1"
                     /translation="MKICIHFLARFLCVGLIFTSAHSLANSNDLSGKVVGGVDASGGE
                     FPFIVSLQDERGHFCGGSLIRKNWVLTAAHCTGLSIKKVVIGLYDRTDLSNTESFTPK
                     RVIVHPKYNANLTDYDFALIELRGDSRFQPIALNTTEIRIPNTPTLMATVIGWGSTKE
                     NSSILPTVMQKANVPLVPRGLCNRSYSGMITERMLCAGYANGGKDACQGDSGGPLVMN
                     MTQSRRLLVGVVSWGEGCAEANRPGIYSKVNKAVSWIEKTLTNTDPL"
     gene            complement(13590..14417)
                     /locus_tag="VIG33_15785"
     CDS             complement(13590..14417)
                     /locus_tag="VIG33_15785"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8272352.1"
                     /translation="MKLKSQYFGLVLLLVLIILSFSAETLSGISFDQQDPESLLLSPG
                     VPHWFGTDSLGRDLFTRVFHGARVSLFVAVFSSVLTMLLGVSIGALAGWLGGWSEKLL
                     IRVLDVLQAIPSFLLVSLLCIFLQNTLEIKDFNLKSLVALAISISLVHWFSIAKITRG
                     QTKQIKNLNYIEAARTLGATPLRIFQKHILPNMQGTLWVLFVMQIPASVLYESMISFA
                     GYGLQSPQVSWGILLQEGWRSLSQFPHLLLLPALVLFLTVLSFHLVFSKKPKDFSLE"
     gene            complement(14414..15334)
                     /locus_tag="VIG33_15790"
     CDS             complement(14414..15334)
                     /locus_tag="VIG33_15790"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY8272353.1"
                     /translation="MRVFFLRKVLETLTALLALMFLTFLLLYKMPGGPFDEEVSLNPA
                     VKENLFKLWHLDKPFLMQFAHYMQNIFQGNLGNSMAQTGKAVTEILQQGFSQTLMLNL
                     LAIALVYFFAFATSFFCALKSGSIFVSGIHFLTVMSISLPSLFLGPVLIYFFGFHLKL
                     LPTAFLSTPWHYILPSLTLSLRPWAQLHLLLENSLKETLQLEFIRMAKAKGLSPWQVL
                     LNHAYKNSLMPVLSLSGPLIVGLISGSFLVEILFSIPGLGQQFVESLNLRDYPVVMGL
                     VLVYGTSLIFLTNIFDICASWLDPRIREQQ"
     gene            complement(15342..17753)
                     /locus_tag="VIG33_15795"
     CDS             complement(15342..17753)
                     /locus_tag="VIG33_15795"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY8272354.1"
                     /translation="MSSASSANSELLSKLRELKQSGKQREAIELLQKEIQKKTTGEDH
                     KLLPLSLAILYFDNGDYEKTIASLDQALKEKSNLEEYAYFYKGMAYFKLNKLSEAQAE
                     FKKVDGHSPNVKLKIDSISMLGQIALQQGNFKEARSHFQKIEKRTRGTEEYPGVIYFM
                     AQAEQGLQNHRGMCKWAEKLYSRYPTSPKVLDWGLDLAENKIGGKATACSSGLEEFKS
                     RVRYLIFAGYDKKAQDEINGIRERLTKEDPYLADQVQAQYFAQEGELTKAYELLKPYY
                     EKKKTNFSYLISFASVSARAGEVQAAVGSYYSAYKMSPHARQAKEALYQSAFLSYQFQ
                     DYDGAARRFKEFMKVYPHSALNLDAKWQLSWIKYLKGDYLGAYKSFSQLQREKGRGRR
                     HHARYPADRMNYWMAMSLYRQEKYEQAKVYFDRLARDPLMGYYSIAAQYRLKKIQSTQ
                     PKIAFRNITETNLGWISRMPSAEFMIPTIDDHGVPFVGRAELETEEGLVMQELAAESA
                     EGEANEAEAGSDEKQVEIAESGGGDKDQNSNPLLAKRFEKARELMTVGLDEWAKWDLY
                     DIEKKTRNRDFMKSLMMEYNSVGYFHRSSYIGQVFFSGQRSSGLNDGRSFWEFAYPKA
                     FSQYVDKYAKDFSVPNELVWGIMRAESQYRKDAISPVGALGLMQVMPFTGYRVANLLG
                     EKDFEPRKLLEPSAAIKMGSRYLQRLMMKFDNTIPLVAAGYNAGPHRVKNWLASFGHL
                     ETDEFIEHIPFLETRNYVKKVVSNCYVYSQLYGNKKDLFSYLGDAVPYKTDGQTVQKE
                     SWDDI"
     gene            17905..19296
                     /gene="radA"
                     /locus_tag="VIG33_15800"
     CDS             17905..19296
                     /gene="radA"
                     /locus_tag="VIG33_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY8272355.1"
                     /translation="MAKSKSKTLYVCQNCGAQKARWEGKCTDCGAWNTLVEELQATEV
                     KTRGWSVAAEGSTPVAAKPVALDQRLEEMHLTRFDTGYAELNRVLGGGLAKGSFVLLG
                     GSPGVGKSTLLLQMAGGLATNKSRVLYISGEESVAQTGSRAHRLGIKTPHIEIAAESN
                     LHTIMDLARHKKPDVLVVDSIQTVYLSDLQAAPGSVSQVRECAGHLMGLAKGDGITVI
                     LIGHVTKDGSIAGPKVLEHMVDCVLSFDGDTSYNFRLLRSLKNRFGAAQELGVFQMNA
                     KGLEEVTNPSELFLEERGDQLIGSAVFASMEGTRPLLCEVQALTLSSPMAMPRRTALG
                     IDVNRLHLLTAVLDRHLDIRLSHSDIFINVVGGLKLIEPAADLAVAAAVLSTEGRKEL
                     DAKTVFFGEIGLTGEVRGVSFVETRLKEADKLGFTHFVLPFSNKRHLGEFKLTKEKKI
                     SYVKNVHELQKLI"
     gene            19308..19715
                     /locus_tag="VIG33_15805"
     CDS             19308..19715
                     /locus_tag="VIG33_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8272356.1"
                     /translation="MSTGSIVSDDSAIFRETYAPNDFIFFEGDIESHFYIVESGKVQI
                     FTKSKTGKRIDILLVEEGESFGEFALLDNKPRSASAQAITEVSLIKVSAEGYEQLLSE
                     LPVWAACMMKSFVVRLKNMTEQLRSSDQFLSRK"
     gene            complement(19796..21277)
                     /gene="glpK"
                     /locus_tag="VIG33_15810"
     CDS             complement(19796..21277)
                     /gene="glpK"
                     /locus_tag="VIG33_15810"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEY8272357.1"
                     /translation="MSNYIISIDQGTTSTRTAIVNQAGGLVGQNNEDFRQIFPEPGWV
                     EHDPEDIWRTTLSTLKKTLDQTRIRGDQIAAIGITNQRETVVVWDRKTGKPIYNAIVW
                     QCRRTQDVCEKLKRNNKEKMVNKKTGLVMDPYFSATKIQWILKSVSGAKERARRGELA
                     AGTIDSFLLWRLTGGTSHKTDVSNASRTMLMNIHTGTWDEELLKLFEVPAALLPEIAS
                     SSGEFGKTKDLGVLPDGIPIAGIAGDQQAALFGQTCFDTGDAKCTFGTGSFILLNTGT
                     KAVTSKARLLTTVAWRLRDQKMIYALEGGAFICGAAVQWLRDGLQFFQHSSEVEQLAA
                     SVASSEGVEFVPALTGLGAPHWNPEARGVLCGLTRGTTKAHVARATLEAMALQNVDIL
                     TAMQKDLGKKLKSIKVDGGASANNLLMQLQADYAATQVVRPQLVESTALGAAYLAGLG
                     VGFWRNSDEIRKIWKSDCSFKVEMAQKDRKLRLERWEKAVSKV"
     gene            21302..22069
                     /locus_tag="VIG33_15815"
     CDS             21302..22069
                     /locus_tag="VIG33_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8272358.1"
                     /translation="MINFFRKPNEKIFNLANLDRDVLLYRVLPRWFMELTRKYFRLEI
                     EGLEHIPKKGAALITPNHSGYMGIDAALLAHILNQEAKRIPRILTHHFWFLTKTTALP
                     AQKMGFTEATFANGVEELQKKRLVVIFPEGESGNFKPTQKMYELQEFHRGFVRMALET
                     GAPIIPCLVIGAEESQINLKKLKLTKFLKGLVIPLPLNFIPLPVKWKIKFLEPIHLPY
                     GPKALKDMDLIHEICEEIQEKMQKAMHEELRKRTSLF"
     gene            22146..22556
                     /locus_tag="VIG33_15820"
     CDS             22146..22556
                     /locus_tag="VIG33_15820"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY8272359.1"
                     /translation="MNIKTVKTLTDFFNSKKEIEIAILYGSFAKGTETNESDIDLAVS
                     LASPLTAQDKISYIDELSLLTHKTIDLVDLREIHVPLSQEVFLTGKVIKNKNKSFWEW
                     LIRRMIYEVSDFLPMRRKEQINRIKKFIGNKSHE"
     gene            22549..22962
                     /locus_tag="VIG33_15825"
     CDS             22549..22962
                     /locus_tag="VIG33_15825"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HEY8272360.1"
                     /translation="MSKDVILNKVENIKNCIERIESKKPFSPQELKANFDLQDIISVN
                     LQRAIQSSVDLANHICSENNLGVPKDMAGVFEILVTKNILSEKLGNELIKSVGLRNVL
                     VHEYDGIDWGIVFIVTENRLQTFRDFCGAIVQLIS"
     gene            complement(22959..24218)
                     /locus_tag="VIG33_15830"
     CDS             complement(22959..24218)
                     /locus_tag="VIG33_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8272361.1"
                     /translation="MRKKEVDILVVGAGIIGTAIGAELSRRGAKVCIIDKDTVGKGCS
                     YGNAGWMTPCFSMPLPMPGMFLKSMKWLMDPQSPLYIKPSFSLDLASWLLNFMKAMNE
                     AQAKRAVDCLVVLSQKSLAEYEKLGQKYPEIRFQKKGLLMVSRTQAGVAAAVEELDYV
                     KNVGVPGRVLNSDDIQGMEPALKGPLLGGVYFSQEAMAEPYLVVQAFAKEIKAHGNEI
                     LENTEFQDLVIANNKIEKVITSSGDSQCEISAKQVVMATGSWSKSLARLLRLRVPILG
                     GKGYAMIVPALEKQPQYPLMLVEKKIAITPRQNTLRIAGTLELVDQDFSITRKRVENI
                     KRGAREFLYLPEELQVQELWAGLRPCTPDGVPLIGYHKNIANLLLAVGHQMLGLQSGA
                     GTGVLVADLLEGKPPFVDMKVLDANRF"
     gene            complement(24227..24433)
                     /locus_tag="VIG33_15835"
     CDS             complement(24227..24433)
                     /locus_tag="VIG33_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272362.1"
                     /translation="MKTSALRKNRRKNNGQVVVEYVLLLVIAVAIAALITKELVQRDP
                     ENPGILVEKWDSILKTIGEDLPDE"
     gene            complement(24447..24878)
                     /locus_tag="VIG33_15840"
     CDS             complement(24447..24878)
                     /locus_tag="VIG33_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272363.1"
                     /translation="MKSSYSTLMQSKFFNPAFNSAIFDGPIRIYFAQFHEALALKIYF
                     MIQQKLSDEMARAKEISKARGANILVMVYPTAESFLLAFEGQEKNTHPLGLEKWNEDV
                     VVGLRGPIEDDHLDLLLETIGLAIQNWKPSLQAVPELQYEA"
     gene            complement(24989..26248)
                     /gene="hutI"
                     /locus_tag="VIG33_15845"
     CDS             complement(24989..26248)
                     /gene="hutI"
                     /locus_tag="VIG33_15845"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY8272364.1"
                     /translation="MAKRNQNLLVTHIDELLTLAPAMKKEGRRSVEEDLGIERNQAMY
                     IENGRIVWIGPQKKIPRGLARQKKTEVFSAQGLTVMPGLVECHTHLIFAGSRAAEFEL
                     RNQGVTYQEIAKKGGGIISTMKQTREASAKTLKQISQQRVREFVSQGVTTLEIKSGYA
                     LDEKNELKCLRVAKSLAGPRIVTTFLGAHAKPPEFNSYKSYLEFLEGKVLPQIKKQKL
                     SQRVDIYVEKGFFDKEDSRHFLKAAQGMGFEIVLHADQLTLSGGADLCVELGALSGDH
                     LLQIGESQIQGLANSQVTCVLLPAADLYMRCNYPPARKLLDAGARVALATDFNPGTSP
                     TQDLSLVGLLARIEMKMTLPEVIVGYTVGASYALNLQSEVGSLELGKCADFMCIDRSW
                     RQLFYSVGERVPVFVYKSGARIYEGSY"
     gene            complement(26323..27300)
                     /locus_tag="VIG33_15850"
     CDS             complement(26323..27300)
                     /locus_tag="VIG33_15850"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY8272365.1"
                     /translation="MSAFEHKIFAQIEPVFVLGSAELNGPAEYTAGWMQEFEENMRIH
                     LEKVLLTPGFPHVLSPKILVEPLAATSAAVEALLEYATKRDADLIIVSSHGRRGLDRF
                     FLGSFAETLLVYSKIPILVIGAGAKIETNFNRILFPTEFGEYAHEVFRRTVSAAKQMG
                     ASIIIYHVVPMPARIVMDTGYYPSLYGVEGEMVSREDFLKIQQEHQSLRAEAWADWAK
                     GEGVNCSFKVESVEEAIDILIYREAVAERADLIMMEGQSGPIKVALLGSIARNVVRTG
                     PCPVLVFPRNALAETEKEGEMLGYEERTPEVSDGLGLADKSSELRDFEV"
BASE COUNT         7852 a         6555 c         5821 g         7102 t
ORIGIN      
        1 ttgacgataa attacacggt aaacgatgca tagttcactc tagaaaagga gttgggtccc
       61 taaccaaaca aatcttccag gagttaaata acccttaata tcttcataaa agatattgcc
      121 aagattattg atacgcagaa agtaatgccc atagacccct gacttatagt ttaaagccac
      181 gttccacaga gtggcctctt tcaaaaccac ccgactagca atatccacat cctcgcggct
      241 tccgacatag ataaactgat tcataaaatg caagggctca aagatttgat aaccgagact
      301 ccaagctcct tgaatcaccg ggcggcggag caaataaagt ccactgtcct catctttcgc
      361 gtaaatctgg gcaatggaaa attgagtgtc ccaggtaact ccctcatatt ggtgcttgat
      421 ctcgacgccc tgggttttaa ctctcttaag atttttatat ttttcagtaa taaaatcata
      481 gtcgatcaaa tcggagaaat ggttatcaaa aacagaaagt ttgaactcct gagtaagccc
      541 ccacctttga atcattccga cttcataagc ctggctatgt tcggcgcgaa gcagaggatc
      601 cccatatcta gagtaaagct gatacaaaac cggccgcttt aacccgctgc tcaagaggcc
      661 atacacgctg cgctgagact ccacccagtg ccactgataa gccagttgat agacggtgag
      721 gcaagctttc gattcccggc agttctcgcg caaaccccag gaaatatctg aactcttaaa
      781 attccagtca ttgactaggt aaacactttc atcatttgtt ttaggattaa aagcgaaagt
      841 ttgcggattt acgaaagctg aattggtaga ggagaagaag tctccctgct cctcattgta
      901 ttcaaagccc acctgccaca aacttttcag ccaacgaatg ttattgcgat ttaaaattga
      961 aatgcgctgg ccataagagg tatccatata ctcgtcaggg ttatagatat cagcgggatt
     1021 tttattttca cgcttcgttt cttggctcgt cagcagaaaa ctgctgccta ccctctcact
     1081 ccaaagagtt ttataacgta ttttccattg cttcgtgcga gcttctgaat gagcgttgat
     1141 atcttcctgc gggggagcat catcattcaa gctgtcttgc agaccattca ccatgatttg
     1201 caattgatcc tgagctgaaa aatccatgct ggcgatcaaa ctgagtgaat ccttctgacg
     1261 accatcgctt tcgagctttc cttcagtccc cctggccacg ctggcattat aagccgacac
     1321 cccctcggtg ctaaaagctg aaccgccaag ggccagttgc catttttcac cgcgaaaacg
     1381 aaattcacca aggccgcgag aggtcttcaa acttcccccc gccagttcta tgctggaatc
     1441 cacatcggaa tcgttctcaa taagcaccac tccacccatg gcttgcgtgc cataaagcaa
     1501 gctttgcggt ccttttaaaa cacgaatgcg attggaaaat tctgagccca gaccaaaggg
     1561 atccgtggca ccgctgggcc cggtgaaatt atcagaacga aaatcattcc acaaaaccag
     1621 cgtttgatct ccattcgccc cgcgcagaaa taaacttccc gtataggtgg tcacctctga
     1681 acccaggacc acaaggcctg gcaaggactt gagagaatca gaggggctga cttcccgatg
     1741 ttcttgcaag atatcgctgg cgatttcttc actggaggga gaagtctgca aaaaagaatt
     1801 ttgtccattc tgaatttgca cacccatccc caaagcaaag aaggggatga ggctcttttt
     1861 cacactgaaa tatttgcgaa ttcttaagaa agtcatgggc tctttgttat ccactaggat
     1921 gtcgtcaatt tttcacggga tcataactct ggtttctttg ccaaacaagc aaagatcact
     1981 aattttaaag tcactttgaa aagacgcaca taaccaacaa ccaggagaaa ttatgagaca
     2041 tcaaagaagc agtagatctg aaaaaagtga acagggaaaa ggaaagcatc aagatcgctc
     2101 tcaggaccga atgcagggcc agcaaagaga agacgaatcc ttaagccgct ggcaagaccg
     2161 tgatgaagat aaagattatc aagatcaagg ctatcgcgca gaggattact ctagattttc
     2221 aagatctgac cgtaatatga atatggaaac caatcaacgt cccggctggg gccgcgaatt
     2281 ttcaggagaa gaaacttctt acggcggcgg acgcggatat tatgatgaag gatcctatgg
     2341 ttcccaacaa ggcgtgagct cacagcgtgg cagccgcagt gaagcccaag gacgttatgc
     2401 gggtcgtggt ccaaaaggct atactcgttc tgacgagcgc atcaaagaag agatttctga
     2461 agctttaact cgtgaccatg aggtcgatgc aagtgatatc gagattgaag tgaaagatgg
     2521 agaagtgact ttgactggat tcgtgccaga aagaaaaatg aaacactccg ctgaagatct
     2581 catcgaaaag tgcatgggcg taaaggacat aactaataat ttacgaattc aaaaagaaga
     2641 aggccgtggc gaggctgaaa gtgagcgcgg cgagaaaaga ggcgccggat cccaagccag
     2701 taaaaaaagc ggcagcacaa gcacgtcaag cttaagtcat taataaagtt ttttaattta
     2761 aatgcgggaa ggattgcagc cagggtttct agaactcaac ttgaaacacc tggcttagta
     2821 aaagaagtct ctcattttta aaagcgcccc ttttttagaa aggagctcaa taaaaaattc
     2881 tatttagttt tgatagttcc agtagcaatt taaaggagtt cctacgggac gtttaactgc
     2941 aaaacgcatt gaatgtcctt ttacttcggc agaaagggaa gaaccagtaa gtgttggcaa
     3001 cgattgtgct gtaaagtcag aagcaccatc atttctaact tcacctttga tgtcgagttt
     3061 gaaattactt gcaagtgtac tcgctgaata aagatcatgc tgtttaaatt caacataagc
     3121 atcacgagtt ccatcttcat ttacttcgtg agcaatatgg agggagcatt gtttttcatt
     3181 taatgggttt gtgtaatatt gatcctcatt gccgccaaaa tccatatatt ttgtccagcg
     3241 agcatgaact gttgaggatg taactaataa aacaataata gcgctaaatt tcttcatatt
     3301 ccttgatacc gttccaaatc ccgtgggatt aatttcccaa tttgcagggg tccggttcct
     3361 tatgactcat tcattatgcg acaactatgc caagacacta aaatggattt agtcgtgaag
     3421 aggcgttgtg aaagaggcct tgtgaaatac ccaaaatggt cgccgcgcga actcaaaaac
     3481 gtcactttga atcccctcgt ccactccatc tggtctatat caccgaacac cctcttcgaa
     3541 gggctggcat cccacttgct gtagtatcta agcacccccc ccggcgacta ggcactcccc
     3601 cccacctggt cgcctttttt ttgcttctgg cattttctcg actgattctc gactctgcca
     3661 gaaacgaaaa atcttctata atattcccat gtgtaaaatc ttctttcttt atatcctgtt
     3721 cactctctca tttcgggccc aggcacttca ggtgatgatc gatcctggtc acgggggcct
     3781 tgactctgga acttctcacc taggagtcca agagaaagat ctggtactca aagtttccag
     3841 ttatttaaag accttgcttg aagaaaatcc gcaattcaat gtcgagatga ctcgcgaaac
     3901 ggatcaacaa ttaagcctgc ggactcgcgt taaaaaagct gaaaaagccc agtctgattt
     3961 attcatcagt ctgcacgcga actctaatcc tgatatgaga atcaaaggga ccgaactcta
     4021 ttttcaaaac tccctgccgc cggatgaaga gagcctgatc ctcgccgaac aggaaaacca
     4081 ggcagagacg acggcggcgg aggccaagac cgccctccaa gactcgccct caaaaaaggg
     4141 cgatatcaga gcgattatag aagacttgca ccgtcaatac cgcgccaaaa gcagtcttaa
     4201 aataacccag attttggccg ctgactggtc tcactccgcc attaaacaag cccctttcta
     4261 tgttgtttcg aaaacctcga tgccctccgt tttaattgaa gtgggatttc tcactcaccc
     4321 tgaagaggca aaaaaactca acactcctaa atatcagcag gaaattgctc agaaaatcta
     4381 caaagctgtc ctccaataca aagaaatgat ggacaaaatt gagggccaag cactagagtg
     4441 aaccctttct cacaaaggat gtttcatgcg tgctcacggg cgtcttcccc atcatttaag
     4501 atcagttaaa atcactcctc atgtttcgga atatgcggaa ggttcagctc tggtggagtt
     4561 cggcagaact cgcgtactct gtacggcaag ttatgaatcc aaagctcccc aatggctcat
     4621 gggctctggc gaaggctggg tgacggcaga atatggcatg ctcccccgct ccactcacca
     4681 acgcatgaag cgcgacaaga cggccagcgg cggtcgcacc caagaaattt ctcgtctgat
     4741 tggccgctct ttgcgcgcgg ctgtggacct caaaggcttg ggcgaaaaac aaattatgat
     4801 cgactgcgat gtgatcaatg ccgatggcgg cacccggacc gcggcgatca ctgggggctt
     4861 cgtggcctta gccttggccc ttaaaaaact tcatggactc agcgaaatca aaagcattcc
     4921 tttaatcagt tatgtgtccg ccgtcagcgt cggtattcaa ggttccacta ttctgttgga
     4981 tcttgattac gatgaagact cctctattgg cacggatatg aattttgtaa tgactgacaa
     5041 aggtcatttt gtggaagttc aagggaccgc ggaagacgtc ccctttactc gcgagcaatt
     5101 gaacgcaatg atggacgtgg cccaacaagg gtgttttgaa cttttcaaag ttcaagaaga
     5161 aatcgtcgga cctattttta aacgcactca gatcagtgga ctgtaaccca ggaataaaat
     5221 atggaacttt ggctcgccac cggaaataaa ggaaaagtga atgagtacaa gctcggctta
     5281 aaagagcttg tggatctcaa ggtttttact caagcagata tcccctcctt tactccccgc
     5341 cctgaagatg gtaaaacttt tctggaaaat gcgcatatta aagcccgaac tcttaaatcc
     5401 gtcaaacctg gagtctgggt tctcggtgaa gactcgggac tcgaggtgga agggcttaat
     5461 aacctgccgg gcattcactc ggctcgctat gccggaccca aggcttctga cagcgaaaac
     5521 gtcgcaaaaa ttttaaagat gatgtccatt cgtcccatcg ccaatcgcaa tgcccgtttc
     5581 gtttgctcca cggtgatttt tacccctggg ggggaagaat ggcattttga aggtattatg
     5641 aaaggcacca tctcttccaa gcctgcgggc caaatgggtt ttggttatga tcccattttc
     5701 ctgcctgagg gacaaactca gacccttgcg gaattaggac ctagttttaa aaatcagttt
     5761 tcgcaccgag cccaagccct taaagctttt ctccaaaaac tgaaatccag cgagagctaa
     5821 tggtaagcaa tgactcatca gtttgaaacc aagtttaggc tgttgaaacc ataatgcgct
     5881 gaagaaaata gcaaattcga agaaattcaa tcgaccaagt agacaaatga caatcccctt
     5941 gctagcctcc ctgcactaaa tgcacttttt aactcaggag actacgagtg atgaagggcc
     6001 taaaaatctt tgcaggaaat tcccatcccc accttgcacg caaggtcgct gaagccgccg
     6061 gtgtcgatct tggcttctcg caggtcagca gctttgctga tggcgaaata caaattgaaa
     6121 tccatgaaag cgtccgcgga caaaatgtct ttgtcattca aagcacctgt cctccagtga
     6181 accagagtta catggaatta tttatcatgg tcgatgccct caaaagagcc tcggctgccc
     6241 aaattacggc ggtgattcct tattttggat acgcccgcca agatcgaaaa gtggccccgc
     6301 gggctccgat ttccgccaag ctgatggcgg atctgttgac cacagctggc gttgatcgtg
     6361 tcgtttcagt cgaccttcat gccacccaaa ttcaaggctt ctttgatggt ccggtggacc
     6421 acctctttgc cattcccacc ttggctcgtg cttggcgcga acaactggga attgggcctg
     6481 aatttgttgc ggtgagtcct gatgcgggcg gagtcgaaag aactcgggcc ttttccaaaa
     6541 ggatcgaaag cagtctcgcc attatcgaca aacgccgttc cgggccaaat gaagccaaag
     6601 cccttcattt aattggcgat gtaactaata aaacggcggt gattattgat gacatgatcg
     6661 acaccgctgg aacccttaca caaggggttg acagccttat caaaaatggg gcaaaacgtg
     6721 tcttcgccgt cgcaacgcat ccggttttat caggtccggc aattgcccga atcaaagaaa
     6781 gcccgattga aaaagtttgg gtgacggaca ctgttccgct gtctgaagcc gcgaaggcct
     6841 gtggcaagtt tgaagtggtc tctgtcgcgc ctttattggc cgaagccatt aaacgcatcc
     6901 acggcaacga ttcagtgagc tcgctctttg attagagttg agagctacag ttgagaaagt
     6961 cctggggact cagaccctat tgaatgagta acaagtgacc agtatccaaa cggagatggg
     7021 tctataaaaa aggaagaacc aatgaaacaa agaattgaac tgactgtaga aactcgcgaa
     7081 gtcggtaaga gccacagccg tgctcttcgt gttgcaaaca aagttccagg agttatttac
     7141 ggtgctatcg aaaacacaaa tgtttttgta gacgcccgtg atgttaataa atacaacact
     7201 cgcgcctatg agaatgcgct cttcaacgta aaaagtacta tcgataaagc taatggcaaa
     7261 gttgttctta ttaaatctgt ggacgtgcat cctttgactc gtcgtccagt tcatattgat
     7321 ttcttcgctt tggatcttac caaaactgtc cgcgtttatg ttgaaatcaa attggaaggt
     7381 aaacctatcg gtctggctga aggtggtttg ctgaacatag ttcaccgtca gatcgaagtc
     7441 gaatgtcttc ctactgaaat ccctgaattt atcactgcag acgtttctaa tctcggcgta
     7501 ggtgatgctc tccacgtttc tgatttgagc gttccgggat ctgttaaagt tatgactcgc
     7561 cccgaagaaa ccatcgcggt ggtagcggct caagaagagg aagcagcagc agttccagcg
     7621 gcagcggccg ctgctcccgc agcaactgca gcagcagttc cagcagccgg tgcaaaacct
     7681 gccgcaggcg cgaagccagc agccggtgca aaaccagcgg ctccagcgaa aaaataattg
     7741 ctttaacgaa gcatctcaaa aagcggcccg caaagccgct ttttttgttt ttggagaaca
     7801 aaaatgtggt tgattgtcgg cctcggaaac ccaggaaatc aatattcact cactcgccat
     7861 aatataggtt ttatggccgt ggattatcat ttaaaaggct tgggtgatcc gccttgtaaa
     7921 aatcaattca aagctgaaat ctttcaaagc aaaattggtg aacagcctgt gatcttttgc
     7981 aagccacaaa ctttcatgaa tctttcgggg gaatcggtcc aaccccttct ggggttttac
     8041 aaaatccctt tggatcattt gatcgtggtg catgatgaaa ttgatcaagc ctttggtcaa
     8101 ataaaaatcc aaaaaaatcg cgggcacggt ggccataacg gaatcaaaag tatttctagt
     8161 ttattgggat ctactgatta cattcgcctt cgtttagggg tgggacgccc cgccatcccc
     8221 actgtgccta tagctgatta tgtattgaac aagttcactg cggaggaatt taatgccctt
     8281 ccttcgtttt taaataaagc cggtgatgcc atagaggcca tcctccgtga tggggtccaa
     8341 aaggcttcga ctagtttcaa tgcttagggt ataaagactt tttaaattga ggagaaatca
     8401 tggcgttaca ggtcggtatt gttggattgc caaatgttgg gaaaagcact ttgtttaatg
     8461 cgctgacttc tgcaaaagca gaggccgcta attatccttt ctgcaccatt gaccccaatg
     8521 tgggcgtggt cactgtcccc gatcctcgta tggaaaaaat cactgccttt gtaaaacctc
     8581 aaagtgtcgt ccccactgtc atggagtttg tcgacattgc agggatcgta aaaggggcca
     8641 gccaaggtga aggcctaggc aatcaatttt taagtcacat tcgtcaaacc gatgctatcg
     8701 tccatgtggt tcgttgtttt gatgatccaa atattattca cgtggccggc aatgtagatc
     8761 ctatccgtga tatggaaatc atcaacacag aattgatgtt agcggatctt gattcagctg
     8821 aaaaacgggt taaacgcgtg gaaaaaatgg caaaatccac gaccgataaa aaaataaaaa
     8881 acgagtttga ggtggccaaa aaagtggtcg aagctcttag caaaggtttg cccgcacgtt
     8941 cggtgggggt cgatgaaaat gaagctccct tcttaaaaga aatgcactta ttaacctcaa
     9001 agcctgtatt gtatgccatg aatgtctctg acacggactt tgccgcgggc ggcaatgatt
     9061 ggacccgagc cgttgaaaaa cgcgcggccg aagaaggcaa caccacgatc atgatctgct
     9121 ctgccatgga agcggaaatc tctcaacttc ccttggaaga acgtaaggac ttcttggagt
     9181 ctttaggagc tgaagagcct ggattgaatc gtctcattcg ggaagcttac aaattgttag
     9241 gccttcacac ttacttcact gcaggcgtaa aggaagttcg tgcttggacg atccgcgcag
     9301 ggaccaaagc tcctcaagcc gccggtgtaa tccacacaga tttcgaaaaa ggttttatcc
     9361 gcgctgaaac ttatcactgc gaagatctct tcaatttgaa gagcgaacag gcggtgaaag
     9421 acgccggtaa ataccgcgtg gaaggcaaag agtatgtcgt caaagacggt gacatcttgt
     9481 tcttccgctt caatgtgtag aaacaaatct aattaaaatt taaaaataaa tccaaagcct
     9541 tagtgggagc tctcctgaac cacctcttcg gcatgaagat tttcgccttg gcgataacga
     9601 tcggccaggg tggaatcatt ggttggaaat gaaaacaaca aggagtgaac attggtggtg
     9661 gagtcttctt gagtgaccac gatgtaggta aggtctttcg tttcatgatc cggatattct
     9721 ttaataaaag tcaccaagac atttccctct aaatcatttt tgcgccagat ttcatccgcg
     9781 ctctcaatgg tttcttcccg caagtccgca aagtcttgaa atagctcttg gggaatatct
     9841 ttatcgccgc gaaccttcat cattgcaata tagagtccca tggaaagagg atccccttca
     9901 ccaagagcat ctccttcgat cgcccctgtt tggacgtatt cgtacttctt gtcataaatc
     9961 aactcgcccc gctgatagtt ctgaagaacg cgcaggtctt tggtcgggaa atgaatgaac
    10021 acaaagctag ggtaagattc ttccgcagtg acataagcca ctgcaatata atgaaaaacc
    10081 tgatcgccga cttcaaaaga tcggataaaa tgatggatag gcagctcggg aaaagtctgg
    10141 gtgtcttgcc aaatttcatc cggctcatcc aacgtgcttt caagataact ttccaattcc
    10201 aactgttttt catctgaaaa atcctcttca gatcgaacgg attgatattc atcttccaag
    10261 gcatcaatac tttccttaaa ataaccaaac agatccgtct cattttcaaa gatcaacccc
    10321 tgagcctcat cgatcacaat atactctttc tctttaattt cctttttagg agctcgagat
    10381 ttggttctgt tttttgcgct ggtttttgcg cttactttca cttttgtcat atctctttgc
    10441 ctcgcttcgc actggaccta atgtcgactt tgtatcgact tagtatcgag attctctctc
    10501 tccacaacaa gtttaaaaag tctcccccat gacagaggcc gtcatgcata gtgctttttg
    10561 tcttgcaagg caaaaccttt ttcttcacca tttgctaaag accaacgcaa ggaggcgggt
    10621 tttgtcaatt atcgaagaaa aaagtttgga gcgcatggag gcccttcttt ctcattctgc
    10681 catgggtgtg catgtactct tcgacaataa atccatcgct aatgttctca gccaagtcaa
    10741 agacgaccgc gacttttatg actttaataa gatgaagaaa gtccaagacg tcatgacgga
    10801 gctgatcgca aaacgcactt atattgagaa aatctcttat ttgaaatctt tagagccaga
    10861 ggcctacgaa atggtggttc gcgcttattt tcatatcgtt gaaaatacgg cccgcgccaa
    10921 tcaagatctg gctcactaac tttaccgacc cggactttag tgatcctttg atattcttca
    10981 ttccttcgct aaaatttttt actgtattac cgacaagtaa atcatgaaaa gcttaggcag
    11041 ttttattttg attctgttcg gactcagtgt cattacccat gccgagtacc gtatgttcac
    11101 cctcaagatc acgaacaaga atacccagga ctaccgtctt gtggatagca ctctcgatcc
    11161 tgttcagtac ccctactatt atcctgttca aaaagatgaa gtcgttcaat acacggatac
    11221 ttggcgctgc tatggccgca ccgggggagg actcccgccc tgtccaaatc taaaatcaaa
    11281 cccagccaca aatccagctc cagtcgcgaa tccttagaga gttctcataa aatttgctat
    11341 tcttgggaat aaggatgagt gaattagaag gaaaccgctt ttccaggaag gagatgcgtt
    11401 catgcccata gaatcttcaa ggacccgccg gacttcgcaa aatgacttga gcgatctcca
    11461 atcggagtat gcgcaaaaaa agaaacaata ctctcatcag caagaagaag agctcaacga
    11521 gctcaaagat ttctacaatg accgtaaaga gcaaactcgc agtcaaggtg aagcggcggt
    11581 caaccatatc cgacgttcac aaaccgagag cctcaatgcc tccaacgagg cccgcaaaaa
    11641 aatcgccgaa cgcagtcagg ctcagatcag tggtattgaa actgattacc gtaaaaaaat
    11701 aaccgacaca caagctaagc gccaagccga tctcgacaca gcccgaatga attcacatga
    11761 gaaactcaat gaaatccaaa aatcccaaca cgacaaagtt gaacaagtcc gttccgaatc
    11821 caccaaggaa atacaaacca ccaaagaacg cttcaatcaa gagcttgccc atacacagca
    11881 gttcactgaa aaacgtatca gcgacattaa aggtaataac gaaaaaaata tgcggcagga
    11941 agttgaaaaa ggacgcattc aacatacaaa gctccatgaa aacctgcaaa aagattacga
    12001 taaagtgaac tcccaggggc agcaggccct cgctgagcga cgtgaaattc aagaaaaaca
    12061 atatcaacga caagacggtg attatgacaa acgttttcac aagcagcacg aacaatggga
    12121 cgctcgtgaa aaaaacatga atgaggaata taacaaacgc attactcatg gcaagaaagc
    12181 ctatgaaacc cagctcaagg atcaacacga gcgttttgac tccgtttata ggaaaaacga
    12241 tatggcccat aaagaatctt taaaaattca aaacaacaat ctacttaaag aacaagttga
    12301 acttaaaaag aagtttttca aagaaaagga aagatacgcc ggcaaagagg acgatccatt
    12361 ttacaagctt caagatcgcg gcaatcgact ccgagaaaat tccgatttct acattattga
    12421 agccttcgtt ccgcaacatg aaaaggacaa catcaaagtg gtcatcaaag atgatacggc
    12481 ggctatttcc ggaaagcgtg cttttataga taaaatcgaa gaagaaggac gcaagctttc
    12541 cacaaatagc tatcaaagtt ttcgagaaga atttaacttc gataaacccg tcatcactga
    12601 aggcatgact cgcgaacgca atggtgatta tgttaccttc tgggttccga agttaaccag
    12661 cttcgacgga agccgtaaac tcagcaaaaa agtttaaagt gggtcagtgt tggtgagtgt
    12721 tttttcaatc caggaaacgg ctttattcac cttactgtat atccctggac gattggcctc
    12781 tgcgcagccc tcaccccaac tcacaacacc cactaaaagg cgtcggctct gagtcatatt
    12841 catgacgagg ggacctccac tatccccctg acaggcatct tttccgccgt tagcataacc
    12901 agcacaaagc attctttccg tgatcatgcc gctgtaactt cggttgcaaa gacccctggg
    12961 aactaaaggc acattcgctt tttgcatgac tgtcggcaat atactggaat tctctttagt
    13021 agatccccaa ccaatcacag tggccatcag cgtaggagta ttcgggatgc gaatctctgt
    13081 ggtattgagc gcaatcggtt gaaagcgaga atcccccctg agttcaatca acgcaaagtc
    13141 gtagtctgtg agatttgcat tgtatttcgg gtggacaatc actcgtttgg gagtgaagga
    13201 ctccgtattg cttagatccg tacgatcata aaggccgatc acaactttct taatgctcaa
    13261 gccagtgcaa tgagcagcgg tcaaaaccca attcttacga atcaaagaac ccccgcaaaa
    13321 atgtccccgt tcatcttgca gagaaacgat aaatggaaat tcccccccgg aggcatcaac
    13381 gccgccaacg acttttcccg aaaggtcatt ggaatttgcc aatgagtgag ccgacgtgaa
    13441 aatcaatcct acacataaaa aacgagctag aaaatgaata catattttca tttttatttc
    13501 ccccttgagt aagcatttca acgctaggga aatcctcaaa tcaaatcctt cggaaatatc
    13561 tcgacctaag tcgaaaatta aatgactgtt tactcgagag aaaaatcttt tggttttttt
    13621 gaaaaaacca gatgaaaact gagcacggtt aaaaacaata caagcgctgg aagcaggagt
    13681 aggtgcggaa actgagagag tgagcgccaa ccttcttgta ataaaattcc ccaactcact
    13741 tgcggagatt gcaaaccata acccgcaaag ctaatcatac tctcgtagag aacgctggcg
    13801 ggaatttgca tgacaaaaag aacccataaa gttccctgca tgtttggtaa aatatgtttt
    13861 tgaaaaatcc gcagcggagt tgcgccgaga gttctggcgg cttcaatata atttagattt
    13921 tttatttgtt tggtctgtcc gcgagttatt ttcgcgatac tgaaccaatg aaccagagaa
    13981 atcgaaatgg ctaaagcaac gagggatttc aaattgaagt cttttatttc caaagtattt
    14041 tgcaaaaata tgcacaggag tgagaccaat aaaaaactag gaattgcctg cagcacatcc
    14101 agaacgcgaa taaggagttt ttcgctccat ccgcccagcc atcccgccaa ggcccctatg
    14161 ctcactccta gaagcatcgt cagcacactt gaaaaaaccg ccacgaacaa agatacccga
    14221 gcgccatgga agactctggt aaaaagatct cgccccaaag aatctgtacc aaaccaatga
    14281 ggaactcccg gagataataa caggctttca ggatcttgct gatcaaatga aattccactt
    14341 aaagtttcag cggagaagga aagtattatc aacaccaaca aaagaactaa tccgaaatat
    14401 tgagatttaa gcttcattgc tgttctcgaa tccgaggatc caaccaggac gcacagatat
    14461 caaaaatatt ggttagaaaa atcaaactgg taccataaac aagaactagt cccatcacca
    14521 ctggataatc acggagattc aggctctcca caaactgttg cccaagtccg ggaatcgaaa
    14581 acaggatctc cacgagaaag gatcccgaga tcaaacctac aatcagcggg ccactcagcg
    14641 acaggacggg cataagggaa tttttataag catgattcag caaaacctgc catggggaaa
    14701 ggcccttcgc cttggccatg cgaataaact ccaactgcaa ggtttctttt aaggaatttt
    14761 ccaacagcag atgcagctgt gcccaaggac gaagacttaa agtcagagac ggcaaaatat
    14821 aatgccaggg cgtggaaaga aaagccgtgg gcaataactt aagatgaaaa ccaaaaaaat
    14881 aaatcagaac cggccccaaa aaaagagagg gcaaagaaat ggacatcacc gtcagaaaat
    14941 gaatgccact gacaaaaatg gaaccgctct tgagtgcgca aaaaaaactg gttgcgaatg
    15001 caaaaaagta aactaaggcg atggccagaa gattcagcat aagagtttgc gaaaatcctt
    15061 gctgcaagat ttccgtgact gccttccccg tctgcgccat cgaatttccc agatttccct
    15121 gaaaaatatt ttgcatatag tgagcaaatt gcatcagaaa aggcttatcc aaatgccaaa
    15181 gtttaaaaag attttctttc acagcgggat ttaaagagac ttcttcgtca aaaggtcccc
    15241 caggcatctt atagagaagc agaaaagtta aaaacatcag agccaacaaa gctgtgagag
    15301 tttcaagaac tttacgcaga aaaaaaactc tcacatccgt cctagatatc atcccacgat
    15361 tccttttgaa ccgtttgccc gtctgtttta tacggaaccg catccccgag ataactaaaa
    15421 agatcttttt tgtttccgta aagctgcgaa taaacgtagc aattggaaac tacttttttt
    15481 acataatttc gagtttctag aaatggaatg tgctctataa attcatcggt ttcaagatgt
    15541 ccaaaggaag ccaaccaatt cttgacccga tgaggacccg cattataacc ggccgccacc
    15601 aagggaatgg tattatcaaa tttcatcatg agccgctgca aatagcggct gcccatttta
    15661 atcgccgcac tgggctctaa taatttacgg ggttcaaagt ctttttcacc caataagttc
    15721 gccaccctgt aacctgtgaa tggcatgacc tgcatcaatc ccagagcccc tacaggagaa
    15781 atggcatctt tgcgatattg gctctccgct cgcataattc cccaaaccaa ctcattcgga
    15841 actgaaaagt ctttggcata tttgtcaaca tactgggaaa aggcttttgg ataggcaaat
    15901 tcccagaagc tccttccatc attcaacccg ctcgatcgct ggcccgagaa aaatacttgg
    15961 ccaatataag aagaacgatg aaaataaccg accgaattat actccatcat gagagacttc
    16021 ataaaatctc tattacgagt tttcttctcg atatcataaa gatcccattt cgcccactca
    16081 tccagcccca cagtcatcag ttcacgggct ttttcaaagc gttttgcaag taagggattg
    16141 gaattttgat ctttatcgcc cccccctgat tccgcgatct caacctgttt ctcgtcagat
    16201 cccgcttctg cttcattggc ctccccttcc gccgactctg ccgccaattc ttgcatcacc
    16261 agtccttctt cggtttcaag ttccgctctg ccgacaaatg gaactccatg atcatcaatg
    16321 gtcggaatca taaattccgc ggacggcatt ctggaaatcc atccaagatt tgtttcagta
    16381 atattgcgga aagcgatctt tggctgagtg ctttgtatct ttttgagtcg gtactgggcc
    16441 gcgattgaat aataacccat cagcggatcg cgtgccaatc tatcgaaata aactttcgcc
    16501 tgttcatatt tttcctggcg ataaagactc atggccatcc aataattcat gcgatccgcg
    16561 ggatatctgg cgtgatggcg acggccacgg cctttttccc tctgcaattg actgaaggat
    16621 ttataagcgc ctaggtaatc tcctttaaga tacttgatcc atgaaagctg ccatttggca
    16681 tcgagattca aagccgaatg aggataaacc ttcataaact ctttaaagcg tcgagccgca
    16741 ccgtcataat cctgaaattg gtaactcaaa aatgcggatt gataaagagc ttctttcgcc
    16801 tgccttgcgt gtgggctcat tttgtaggct gagtaatagg agcctacagc ggcctgaact
    16861 tctccggccc gagcggacac cgaagcaaaa ctaatcaaat aagaaaagtt tgttttcttt
    16921 ttctcataat aaggtttaag cagctcataa gctttggtga gttccccttc ttgggcgaaa
    16981 tattgagctt ggacttggtc cgctaaataa ggatcttcct ttgtgagacg ttcgcgaatc
    17041 ccattgatct catcttgagc ttttttatca taacccgcga aaatcaagta ccgaacccgg
    17101 gatttaaatt cttccaaccc ggaagaacaa gccgtcgcct taccgccgat tttattttcc
    17161 gccagatcca agccccaatc taagaccttg ggagaagtcg gatatcgtga ataaagcttc
    17221 tcagcccact tacacattcc gcgatggttc tgcaaccctt gttccgcttg cgccataaag
    17281 taaataacac cgggatactc ttccgtgcca cgcgtccgtt tttcaatttt ttgaaaatga
    17341 cttctggctt ctttaaaatt gccctgttgc agtgcgattt gtcccagcat ggaaatagaa
    17401 tcaattttca atttcacgtt cggagaatgc ccatcgactt ttttaaattc cgcctgagct
    17461 tcagacagtt tattgagttt aaaataagcc atccctttat aaaaataagc gtattcttca
    17521 agattgcttt tctctttcag cgcttgatcc aaactggcaa tagttttttc gtaatctcca
    17581 ttatcgaaat aaaggatcgc caaactcagc ggcagaagtt tatgatcttc gcctgtggtt
    17641 tttttttgaa tttctttttg caagagctcg atcgcttccc tctgttttcc actttgttta
    17701 agttcccgaa gctttgacag caactcggaa ttagccgaag aggccgaact cacgagaatc
    17761 gccgttgaaa ttgaaataga gcctaaaatc tttttggatc tgcttgtggg tttcggtttg
    17821 gatttgaaca ggggctcctc ctgagacctt aattattaaa ctacacttat tgaactacct
    17881 ctagattaat gataaccaat atccatggca aaatcgaaga gcaaaacact ttatgtgtgt
    17941 caaaattgcg gagcgcaaaa agcgcgatgg gaaggcaaat gcaccgactg tggtgcatgg
    18001 aataccttag tagaggagct gcaagccact gaggtgaaaa cccgcggttg gtctgtagcc
    18061 gctgaaggat ccacccctgt tgccgcaaag ccggtggcgc tagaccaaag actagaagaa
    18121 atgcatttga ctcgctttga tacgggctat gcggaactca atcgcgtttt aggcggcggc
    18181 ctggctaaag gaagtttcgt tttgctcgga ggttcgccag gagtgggaaa aagtactttg
    18241 cttttgcaaa tggctggggg acttgcaacc aacaaaagtc gcgttcttta tatttccggt
    18301 gaggaaagcg ttgcgcagac aggctcccgt gctcaccgct tgggtattaa aactccgcat
    18361 attgaaatcg cggcggagag caacttgcac accattatgg atttagctcg tcataaaaag
    18421 cccgatgttt tggtcgtgga ttccatccaa acggtttatc tttcagatct gcaagccgct
    18481 cccggatcgg tttctcaagt tcgtgagtgc gcgggccatt tgatgggact ggctaaaggg
    18541 gatggaatca ccgtgattct cattggtcac gtcaccaaag atggcagcat tgcgggcccc
    18601 aaagtcctcg agcatatggt ggactgcgtg ctatcgtttg atggtgatac ctcttacaat
    18661 tttcgtttgt tgcgctcctt gaagaatcgt ttcggagcgg ctcaagaact cggcgtcttt
    18721 caaatgaacg ccaaaggtct agaggaagtc actaatcctt cagaattgtt tcttgaagaa
    18781 cgaggagatc aactcattgg ctccgccgtc tttgcctcta tggaaggaac gcgtccttta
    18841 ctttgtgagg tccaagcttt gaccctctcc agtccgatgg cgatgcctcg tcgaaccgcc
    18901 ttaggcatcg atgtgaatcg attgcattta ctcacggcgg tcctagatag acatcttgac
    18961 attcgactga gccacagcga catttttatt aatgtggttg gaggcttgaa attgattgaa
    19021 cctgctgctg acttagctgt ggctgcggcc gttctttcca ctgaaggccg taaagagctt
    19081 gatgctaaaa cggttttctt cggagagatt ggtctgaccg gcgaagtgcg gggagtttct
    19141 tttgtggaaa cgcgcctgaa ggaagcggat aagttgggtt tcactcattt tgttttaccc
    19201 ttctccaata aaaggcactt aggtgagttt aagctcacca aagagaaaaa gattagttat
    19261 gtgaagaatg tgcatgaact gcagaagctg atataggaga aggctttatg tccacaggct
    19321 caattgtatc cgatgattct gctatttttc gcgaaaccta tgcaccgaat gattttattt
    19381 tctttgaagg ggatattgaa agccatttct acatcgttga atccggcaaa gtgcaaattt
    19441 ttactaaaag caaaactggc aagcgcattg atattttatt ggttgaagaa ggtgaatcct
    19501 ttggcgaatt cgcccttctc gacaacaagc cccgctctgc ttccgctcaa gctatcaccg
    19561 aagtgtcctt gattaaagtt tccgccgaag gatatgaaca attgctttca gagcttccgg
    19621 tctgggcagc ctgtatgatg aagtcttttg tcgtgaggct taaaaatatg accgaacaac
    19681 ttcgtagcag cgatcaattc ctcagtcgca aatagatatt ttcttggttt attccaaaat
    19741 ttaagtcagt taagaatatt tttactcagt gcatccgaaa aaggtacggc gtaccttaaa
    19801 ctttgcttac ggctttttcc caacgttcta ggcgcagttt gcgatctttt tgagccatct
    19861 ccactttgaa ggagcaatca cttttccaga tttttcggat ttcgtcgctg ttgcgccaga
    19921 agcctacacc cagccctgca agatatgcgg caccaagggc cgtgctttcc acaagctgag
    19981 gtcgcaccac ttgagtggcc gcataatccg cttgcaactg catcaagagg ttattggctg
    20041 aagctccgcc atcgaccttg atacttttta attttttgcc caggtccttt tgcatcgccg
    20101 tcaagatatc gacgttttgc aaagccatcg cttccaaagt tgcacgagcc acatgagctt
    20161 tcgtagttcc gcgcgtgagt ccgcagagaa caccgcgagc ttctggattc caatgcgggg
    20221 cccccaggcc agtcaatgcc ggaacaaatt ccaccccttc actgctggcc acggaagcgg
    20281 caagttgctc cacctcagag ctgtgttgaa aaaattgaag cccatctctc agccactgaa
    20341 ccgcagcacc gcaaataaaa gctccaccct cgagagcgta aatcatcttt tgatcacgca
    20401 aacgccaggc caccgtcgtc aacaaccgag ccttggaagt caccgcctta gtgcccgtat
    20461 tgagcaggat gaaactgcct gttccaaagg tgcatttcgc atccccggta tcaaagcaag
    20521 tttgtccaaa aagagccgcc tgctggtcgc ctgcgatgcc agctatagga attccatccg
    20581 gcagaacgcc aagatctttg gttttaccga attctccact ggaagaagca atctccggca
    20641 gtaacgctgc gggcacttca aaaagtttta aaagttcttc atcccaagtg ccggtatgaa
    20701 tattcatcag catcgtgcga gaggcattgc tcacatccgt tttgtgagaa gtgccgcctg
    20761 tcaatcgcca gagaagaaaa ctatcgatgg ttccggcggc gagttcgccc cgtcgagccc
    20821 gttcttttgc ccctgaaacg cttttaagaa tccattgaat tttagtggcg gaaaaataag
    20881 ggtccatcac caggccggtc ttcttattca ccatcttctc tttattattc cgtttcagtt
    20941 tctcacaaac gtcttgggtg cgacggcact gccaaacaat ggcattatag ataggctttc
    21001 cggtcttacg atcccacacc accaccgttt ctctttggtt ggtgatacca atggcggcaa
    21061 tttgatctcc gcgaattcgg gtttgatcga gtgttttttt caaagtactg agcgtggtcc
    21121 gccagatatc ctcgggatca tgttccaccc aaccaggttc tggaaagatt tgacggaaat
    21181 cctcattatt ttgaccaaca agacctccgg cttggttgac aatcgcagtg cgagtgctag
    21241 ttgtaccttg atcaatagaa atgatataat ttgacataca taactcctgg gcccaaccat
    21301 catgataaat ttttttagga aacccaacga aaaaattttt aatctggcaa atcttgatcg
    21361 agatgtctta ctgtatcgcg ttctgccccg ctggttcatg gaactgaccc gcaaatattt
    21421 ccgcttggaa atcgagggcc tagagcatat tcctaaaaaa ggggccgcac ttattacccc
    21481 caatcattcc ggatatatgg gaattgatgc agcattgctt gcgcatatcc tcaaccaaga
    21541 agccaaacgc attcccagaa tcttaactca tcacttttgg tttttaacaa aaaccacggc
    21601 tttgcctgcg caaaaaatgg gatttaccga agccaccttt gccaatgggg tggaggagct
    21661 acaaaaaaaa cgcttggtag tgatctttcc agaaggcgaa agtggaaact ttaaaccaac
    21721 acaaaaaatg tacgaactgc aagaatttca tcgcggtttt gtacgcatgg cccttgagac
    21781 tggcgctccc atcatacctt gtctggtcat tggcgcggag gaaagtcaga tcaatcttaa
    21841 aaaattgaaa ctcaccaagt ttctaaaggg cctggttatt cccttgccgc tcaattttat
    21901 tcccctgccg gtgaaatgga aaatcaaatt cttagaacct atccatttgc cctatggccc
    21961 taaagccctt aaagatatgg atttgatcca cgaaatctgc gaagagattc aagaaaaaat
    22021 gcagaaagcc atgcacgaag aacttaggaa aaggacctcg cttttctaat aactttttca
    22081 agtcgagcat aacgctcact cacaaaggac ttgattttac agcttcgtcc tcatctaatg
    22141 actctatgaa tattaagacg gtgaaaactc ttaccgattt tttcaactcg aaaaaagaga
    22201 tcgaaatagc gattctctat ggctcctttg ccaaaggcac cgaaacgaat gagagcgata
    22261 tcgacctagc tgtttcttta gcctcacccc ttaccgctca ggataaaata agttacatcg
    22321 acgaattaag cctgctcact cataaaacca ttgatcttgt ggaccttcga gaaatccatg
    22381 tccccttaag ccaagaagtg tttttaacag gtaaagtgat aaaaaataaa aacaaaagtt
    22441 tttgggagtg gttgatacga cggatgattt atgaagtctc agatttttta cctatgaggc
    22501 ggaaagaaca aataaacagg atcaaaaaat ttattggaaa caaaagccat gagtaaagat
    22561 gtgattttaa ataaagtaga gaatattaaa aattgtattg aaagaattga aagtaaaaaa
    22621 ccattcagtc cacaggaact aaaagccaat tttgacttac aagacatcat ctcggtgaat
    22681 ctccaaagag ctattcaaag ctcagtggat cttgccaacc acatttgttc ggaaaataat
    22741 ctcggcgtcc ccaaagacat ggctggggtc tttgaaattt tagtgacaaa aaatatcttg
    22801 tccgaaaaac ttggcaatga attgattaaa tctgtagggt tgcggaatgt tttagtccat
    22861 gaatacgatg gaattgattg gggtattgtg tttatagtca cggaaaatcg actccaaacc
    22921 tttcgggact tttgtggcgc aatagtccaa ttgatttctt aaaatcgatt ggcgtcgagg
    22981 actttcatat ctacaaaagg gggttttccc tctaaaagat ccgccactaa cactcccgtt
    23041 cctgctccgc tttgcaaccc cagcatttga tgtccgacgg ccaaaagcaa atttgcgatg
    23101 tttttatgat aaccaatcag cggaacccca tccggagtgc aaggacgcag gcctgcccac
    23161 agctcttgca cttgcaattc ttcgggcaag tacaagaact cgcgagctcc gcgtttaata
    23221 ttttccactc gttttcgcgt gatggaaaaa tcttgatcca ccagttccaa ggtgccggcg
    23281 atgcgcaggg tattttgtct cggagtgatg gcgatttttt tttcgaccag catcaaggga
    23341 tactgaggct gcttttccag cgcgggaaca atcatcgcat agcccttgcc gcctaaaata
    23401 ggaactctga ggcgtaagag tcgcgccaaa ctcttcgacc aactgcccgt ggccatcaca
    23461 acttgcttcg ctgagatctc gcattgggaa tcaccgcttg aagtaatgac cttttcgatc
    23521 ttattattgg caatcactaa atcttgaaac tctgtatttt ctaaaatctc gttcccatgg
    23581 gctttgattt ctttggcaaa agcttgaacc accagataag gttccgccat ggcctcttga
    23641 gaaaagtaaa ctcctccaag aaggggcccc ttcagcgcag gctccatgcc ttgaatatca
    23701 tcggaattta aaacgcgtcc tggcactccc acatttttca cgtaatccag ttcttccaca
    23761 gccgcggcaa cacccgcctg agtgcggctg accatcaaga gacccttttt ctgaaaacga
    23821 atctctggat atttttgacc caacttttcg tactcagcca agctcttttg cgaaagcact
    23881 accagacaat ccacggcccg cttggcttgc gcctcattca tcgccttcat gaaattgagc
    23941 aaccaagaag ccaggtcgag agagaacgac ggtttgatgt agagcgggct ttgaggatcc
    24001 atcaaccact tcatggattt taaaaacatc cccggcattg gtaagggcat ggaaaaacaa
    24061 ggagtcatcc agccggcatt tccgtaagag cagcccttac ccacggtgtc cttgtcaata
    24121 atgcagactt tggcaccgcg acgagaaagt tccgcaccaa tggcggtgcc aatgatgcca
    24181 gctccaacaa ccaagatatc aacttctttt tttcgcatgc ttcaactcat tcgtctggca
    24241 gatcttcgcc tatggtcttt aaaatggagt cccacttttc cactagaatt cctggattct
    24301 caggatcccg ctggaccagt tctttggtga taagagccgc gatcgcaacc gcaatcacca
    24361 gaagcagaac atactccacc accacctgac cgttattttt tcggcgattt ttcctaagag
    24421 cggatgtttt catggaagaa aacagcctaa gcttcgtact gaagctctgg aactgcttga
    24481 agagaaggct tccaattttg aatcgctaag ccaatggttt caagcaaaag atccaaatga
    24541 tcatcttcaa taggtccgcg cagtcccacc accacatctt cattccactt ttctagtccg
    24601 agtggatgcg tatttttctc ttgcccctca aatgccagca agaaactttc cgccgtcgga
    24661 tagaccatca ccagtatatt agcgcctctg gctttggaaa tttctttagc gcgggccatt
    24721 tcgtcgctta atttctgctg gatcataaaa taaattttca aagccagcgc ctcatgaaat
    24781 tgggcgaagt agatacgaat gggtccgtcg aatatggccg aattaaaagc cgggttaaaa
    24841 aacttcgact gcatcaaagt agaataagac gacttcattt gtttttcctc tcgaagtatt
    24901 gaatcgcttt cagttcctaa gactaggaat acttgcagta gtttttcaaa gactttttta
    24961 agaatcttaa attttaggat tcgatgcgtt aataagagcc ttcataaatg cgcgctccac
    25021 tcttatatac aaagaccggc acacgctcgc caacactgta aaataactgc cgccagctcc
    25081 tatctatgca cataaaatca gcacacttgc cgagctctaa agaccctact tcagattgca
    25141 aattaagtgc atatgaagct cctactgtat agcccacaat cacttcggga agagtcatct
    25201 tcatttcgat gcgagccaac agacccacca ggctcaggtc ctgcgtaggt gaagtgccag
    25261 gattaaaatc cgtcgccaag gccactcgcg ctcccgcatc aaggagcttt cgtgcgggag
    25321 gataattgca tctcatatac aaatccgccg ctggcaacaa cacacacgtc acctgcgaat
    25381 tagcaagacc ttgaatttgt gattcaccaa tttgcaaaag atgatctccc gataaagctc
    25441 ctagctccac acaaagatcc gctcctccgc ttaaggtcaa ctgatcggca tggaggacaa
    25501 tttcaaaacc cattccttga gcggccttta aaaagtgtcg ggaatcttct ttatcaaaga
    25561 agcctttctc tacataaata tccacgcgct gagaaagttt ttgcttttta atttgcggca
    25621 aaactttgcc ctctaagaat tccaaatagg atttataaga attgaactca gggggcttgg
    25681 catgggcccc caaaaaagta gtaacgatcc gaggacctgc taaagatttt gccaccctca
    25741 agcatttgag ctcattcttt tcatccaacg catagcctga cttaatctct aaagtagtga
    25801 cgccctggga aacaaattct cgcacccttt gttgagaaat ttgctttaaa gttttcgccg
    25861 aagcttcacg ggtttgcttc atggtggaga taatgccccc gccttttttg gctatttctt
    25921 gataggtgac cccttgattt cgaagttcaa attccgcagc ccggcttccg gcaaaaatta
    25981 aatgagtatg gcactccacc aatccgggca taaccgtcag accttgagcg gaaaacacct
    26041 cggttttttt ctgtcgagcc aatcctctgg gaattttctt ctgagggccg atccaaacaa
    26101 tacggccgtt ctcgatatac atggcttgat ttcgctcaat gccaagatct tcttcgacac
    26161 tccttcgccc ttcttttttc atcgccggag ccaaggtcaa taattcatca atgtgggtca
    26221 ctagcaagtt ttgattgcgt ttagccatga ttttttgtag cggtcttcaa tcggcgtggc
    26281 aatctctaaa tttaaatcaa acttcaaagt caaacttcaa agtcaaactt caaagtcccg
    26341 tagttcggaa ctcttatccg caagccctag accatctgag acctcgggag ttctttcctc
    26401 atatcccagc atttcacctt ctttttccgt ctcagccaat gcattacgag gaaagactaa
    26461 tacagggcaa ggtcctgttc ttaccacatt ccgggcaata ctgcccagaa gcgccacttt
    26521 gatcggacca ctttgtcctt ccatcataat aagatctgcc ctctccgcaa ctgcctctcg
    26581 atagattaaa atatcaatcg cctcctcgac gctttccact ttgaagctgc aattcacacc
    26641 ttcacccttg gcccagtcgg cccaagcttc agcgcgaaga gattgatgct cttgctgaat
    26701 ttttaaaaaa tcttcccggg aaaccatctc tccttcgacg ccatacaggg aaggataata
    26761 gcctgtatcc atcacaatcc gcgccggcat cggaaccaca tgatatatta ttatcgaagc
    26821 ccccatttgt ttggcagcgg aaaccgttcg gcgaaaaact tcgtgagcat actcaccaaa
    26881 ttccgtagga aataaaatac ggttgaaatt agtttctatt tttgcgcccg ctcctatcac
    26941 taaaatagga atcttcgaat aaaccaacaa agtctcggca aaactcccta aaaaaaagcg
    27001 atccaagcct cttcgcccat ggctgctgac gataattaaa tccgcatcac gtttcgtcgc
    27061 gtattctaag agagcttcca ccgccgcact tgtggccgcc aaaggctcta cgagaatttt
    27121 tggacttaag acatggggaa agccaggggt cagcaaaacc ttttccaagt gaatgcgcat
    27181 attttcttcg aattcctgca tccaaccggc cgtgtattcg gcaggaccat tcagctctgc
    27241 ggaacccaac acgaaaacgg gctcaatttg cgcaaatatc ttatgctcaa aagcactcaa
    27301 tacagcgacc gctttttctt gaagatctta
//
LOCUS       DATBTE010000410         7688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_808948, whole genome shotgun sequence.
ACCESSION   DATBTE010000410 DATBTE010000000
VERSION     DATBTE010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808948"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /locus_tag="VIG33_15855"
     CDS             <1..1472
                     /locus_tag="VIG33_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272366.1"
                     /translation="VHNLLAARRVTGPIEITGGLAVTNEHHIEVRRSDEGVFREMGQV
                     DLMKGSYSIDIDEASGILIARLIDKSGRILGEGEIRLSQLQAGADRLIKGPRLEISPS
                     PVWTGKVSRYYSNPKKEETPPTHVTTFSGQNVVTVGRNGEISIEKLSKNSSTVVRAET
                     PEHMPSSKIMIAGAKSFGIPLFPNSMMEALKSIVSDQKSGAYNELAETSVVWGTATLD
                     GKPLSGVTVISETEPEVRAIYFNEFMIPDPKLSATSSIGLYAFIGLPSGFHALLGQRG
                     DAYFGHQNVDVEAGSVAIGDIESTLRTEPVRIRAFDAFGGEPVTLTANLQSFSQPVDI
                     IEGSASVVLPQISRLSMMYTETPQKYLSANYFYNDQDSYIHLPMISRDWLGEIRTFAK
                     INELSQAGTIVGFFGEENFEAYLAGQTKESPAEIVYFDATGKLIDGHRGLLGGGFIIF
                     NVPYGIQEVVVVGDNSEKIFSKVVPVDINTVSVLNFTSF"
     gene            complement(1459..2832)
                     /gene="murD"
                     /locus_tag="VIG33_15860"
     CDS             complement(1459..2832)
                     /gene="murD"
                     /locus_tag="VIG33_15860"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY8272367.1"
                     /translation="MWISEYSFFDFDSESILCKEPILSHYLRMIQTPVAIIGMAKSGQ
                     SAERLLLASGHKKEDVLTFDQKAPAQFSDPLELINKGRPKTLVISPGVPLKSSWIVEA
                     QKSGIIITSEINLACEYLANEKIIGVTGSLGKSTTVSLLGAGVTAFDKNAFVGGNLGT
                     PFCEYAYGIVTGKRPRAQWIILELSSYQLENCKSLHLDHSVITFLSANHMERYEHLED
                     YYLTKWRIVDKTKKTLFLNKNGGHLLMFAKKQSEFSRCRVVSSEDSDLAPYSLSQSAL
                     LGSHNQDNIALAAQIALQCHWPLSSIKAMKTFQGLEHRLENMGFFNGVRYINDSKATA
                     LDSVLTAVEVAHKYLSPLATLHVLLGGRNKNLPWEELIPLGNLSGIKFLFFGECKEIA
                     STKSQLLGPQFAKLEEAVTYVMGQAKSDDIVLLSPGGTSLDEFKGFEDRGRFFKEKVN
                     SFYLKKK"
     gene            2811..4262
                     /locus_tag="VIG33_15865"
     CDS             2811..4262
                     /locus_tag="VIG33_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY8272368.1"
                     /translation="MNIQKSTCFKNAKYTAVVSDLHLCEAEPVNSKFPLWKKFKTKQF
                     FFDNIFTTFLQEIESRAKGESVELILNGDIFDFDSVLRLPQEPIFRISLLEKRCGLFP
                     REERSCYKIKVILEDHAPFFQALHSFVCRGHRVVFVIGNHDLELHFPEVQKEIRHALD
                     LTEEQQNQVRFVEWFYISNEDTLIEHGNQYDPYCLCEDPINPYVRGYNYVSLKLPFGN
                     LACRYIMNGLGFFNPHVDSNYIMTLKEYVTTFLRYMARAQPFLIFTWLSGSVVTLLYS
                     FKDRLAAPIRNPLKIEERIEQIAEKANAEPRMVRELKELFVAPAASNPFLLARELWLD
                     RAFMIGIAFFLIFELFIFIRQVYDISFFWAFIPLFFMLPFFLFYSQSITSMVSSYKEP
                     NERVLAMTSAITRVKRIIYGHTHSVRHEMIGAVEHLNSGCWSPAFLDIECTKPIDQKT
                     YVWLSPQESGERQAELYKFIEGRSHLFMSTSNR"
     gene            4368..4562
                     /locus_tag="VIG33_15870"
     CDS             4368..4562
                     /locus_tag="VIG33_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like protein"
                     /protein_id="HEY8272369.1"
                     /translation="MSEVLVVTSKVKKIIKEKGGMNTSAETIDVLSKAIERLCLKGVD
                     SAKADGRKTVMARDIVIDHL"
     gene            complement(4635..5087)
                     /locus_tag="VIG33_15875"
     CDS             complement(4635..5087)
                     /locus_tag="VIG33_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272370.1"
                     /translation="MSDKIVQYGPLTPQELDKLVHELQLRNIPFEIQKDEEAEKKFKS
                     NNFSNIVNQISYRNEQYLGQIFYITLATKDLPRMEEHLKELGFPIDLPEFSGESPPEL
                     DNDERENVFVKARAEKTNFNRRMIAWIFLIGMLCMMFISYALTVLNQP"
     gene            complement(5090..7099)
                     /gene="ligA"
                     /locus_tag="VIG33_15880"
     CDS             complement(5090..7099)
                     /gene="ligA"
                     /locus_tag="VIG33_15880"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY8272371.1"
                     /translation="MSKKRHEELKKIIAEHDYNYYVTDKPLISDYEYDQLFAELLELE
                     KNEALLGHLDLSDSPSQRVGGAPLGAFEKVTHRKPMLSLANSYSPEDILEFDERVKKF
                     LKREGPVVEYYVEPKFDGLSMELVYEKGFLIRAITRGDGYTGEDVTQNIRTIKSIPLK
                     LETSRPPELLEVRGEVLILKADFLKLNEQQQENGQPSFANPRNAAAGTIRQLDPKIAG
                     SRRLKFFAYALGEVDGIKFENQADIGTYFQKVAIPTATGSHPELMRICKSPKEAIDYY
                     HFIEKERPHLPFDIDGIVIKVNALRQQEDLGLVARSPRWATAAKFKPEQAQTIVENIV
                     IQVGRTGALTPVAIMKPVRVGGVTVTNATLHNQEEIDRKDIRLGDTVIIQRAGDVIPE
                     IVNVDLAKRPKKSEPFLLPNKCPACGTEVHKLEGEVVTRCTNPLCIAIIKESLKHFAS
                     RRAMNLDKIGDRLIETLVDQKLVRKFSDLYRLKKENLLPLERQGEKSVENILSSIEAS
                     KNTTLARFIFSLGIRFVGEQTARHLAEHFGTIENFLQAKENDLLLIPEIGPKVAKGIL
                     EWTSNPKLVKEVKDLVLFGVSIRSPQRSTTGMLAGKSFLITGTLPVKRDDAKDFIEKN
                     GGKILGSVSSKLNYLVVGDDPGSKLDKAQGLGVKVISWEELQGMV"
     gene            7184..7465
                     /gene="gatC"
                     /locus_tag="VIG33_15885"
     CDS             7184..7465
                     /gene="gatC"
                     /locus_tag="VIG33_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEY8272372.1"
                     /translation="MIDKKTIEHIAKLARLEITEDEATEYSQQLGKAMAHFEQISKVD
                     TTGIEPLVTPAEIEDFWREDLVKQEFTPEDMVSNAPSKAGHLFKVPPVV"
     gene            7476..>7688
                     /locus_tag="VIG33_15890"
     CDS             7476..>7688
                     /locus_tag="VIG33_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY8272373.1"
                     /translation="MDLIQASIVDIADAVKAKKVSAKEITTQFIKRAESINPKLNAYT
                     SFNNGALQDAEAIDARIAKGEDAGVLA"
BASE COUNT         2148 a         1557 c         1664 g         2319 t
ORIGIN      
        1 ctgtacataa cttattagcg gcgcgacgag tgactggccc aattgaaatt acagggggtc
       61 ttgcggtcac caatgaacat cacatagaag ttcgtaggtc tgatgagggc gtgtttcgtg
      121 agatggggca agttgatcta atgaaaggct catacagtat tgatattgat gaagcatccg
      181 gaattcttat agctcgctta attgataaaa gtggcaggat tttgggtgaa ggtgagattc
      241 gcctaagtca attacaggcg ggcgctgacc gtttgattaa aggtcctcga ttagagattt
      301 cgccaagtcc agtttggaca ggaaaagttt ctcgctatta ttcgaatccc aaaaaggaag
      361 agactcctcc gactcacgta accacgttta gtggtcaaaa cgtcgttact gttggtagaa
      421 atggggagat ttccatagaa aagctctcta aaaattcctc tacagtagtg cgtgctgaaa
      481 ctccagaaca tatgccatca agcaaaatca tgatagctgg agcaaaatct tttggtatac
      541 ctctatttcc aaactcaatg atggaagctc ttaaaagcat tgtatcagac cagaaaagcg
      601 gtgcttataa tgaattggcg gaaacttctg tggtgtgggg aacggctacg ttagatggaa
      661 agccactttc aggggtgaca gtgatttcag agaccgaacc tgaagttcgc gcaatttact
      721 ttaatgagtt catgattccc gatcccaaac tctcggctac aagctccatc ggtctctatg
      781 catttatagg tttgccgagt ggttttcatg ccttattggg tcagagggga gatgcttact
      841 tcggacatca aaatgtggat gtagaagccg gcagtgtggc tattggcgat attgaaagca
      901 ctcttcgcac tgaacctgtt cgtatacgag cttttgatgc cttcgggggt gagccagtta
      961 cactcacagc gaacttacag agttttagcc aaccagtaga cattatcgag ggatccgcct
     1021 ccgttgtgct tcctcagatt tcacgtttaa gtatgatgta tacggaaact ccacaaaagt
     1081 atcttagtgc aaattatttt tataatgatc aagactctta tattcatttg ccaatgatct
     1141 cacgggattg gttgggagaa ataagaacct tcgctaagat taacgagctt tcacaagctg
     1201 gaacgattgt tgggttcttt ggtgaagaga attttgaagc gtatttagcg ggtcaaacca
     1261 aagagtcgcc cgctgaaatt gtttattttg atgcgactgg aaaacttatt gatggtcatc
     1321 gaggccttct gggtggtggt tttataatat ttaatgttcc ttacggcata caagaagtgg
     1381 tagtggtggg ggataattct gaaaaaatat tttcaaaagt agttcctgtg gatattaata
     1441 cggtctcagt tttaaatttt acttcttttt aagatagaag ctgttaactt tttctttaaa
     1501 aaatcgtccg cgatcttcaa atcctttaaa ctcgtctaga ctagttccgc caggactcag
     1561 aagaacaatg tcgtcggact ttgcctgacc cataacataa gtaacggctt cttcaagttt
     1621 tgcaaattgc ggacccaata attgagattt tgtgctagct atttctttgc attctccaaa
     1681 aaataaaaac ttgatgccgg aaagatttcc taagggaatt aattcttccc agggaagatt
     1741 cttatttcgt cctcctaaaa gaacatgcag agtagctaat ggcgaaagat atttatgagc
     1801 cacttcgaca gctgtcaaaa cactatccag tgcggtcgct ttactgtcat tgatgtagcg
     1861 cactccattg aaaaacccca tattttctaa tcgatgttct aaaccttgaa aagtcttcat
     1921 agcttttatg gacgaaagag gccaatgaca ctgcaaagcg atctgtgctg ccaaggctat
     1981 gttgtcttga ttgtgagagc ccaaaagcgc gctttgggaa agagaatatg gagcaagatc
     2041 ggaatcctca ctggaaacga ctctgcagcg cgagaattca gattgtttct tggcaaacat
     2101 taaaagatgt ccgccatttt tgttaagaaa cagggttttc tttgttttgt ctactattcg
     2161 ccattttgtt aaataataat cctcgagatg ttcatatctc tccatatgat tggcggataa
     2221 aaaggtgatc acggaatgat caagatgaag acttttacag ttttccagtt gataactgga
     2281 aagctccaaa ataatccatt gggcgcgcgg tcttttgcca gtgacaatgc catatgcgta
     2341 ttcacaaaag ggcgttccta aattaccgcc gacaaaagcg tttttatcga aagcagttac
     2401 gccagcccct aataaagaaa cggtggtgct ctttcccagg gaacctgtga ctccaattat
     2461 tttctcattg gctaaatatt cgcaggctaa attaatttcg cttgtgatga tgattccaga
     2521 tttttgcgcc tcaacaatcc atgaggactt gagggggacg cccggtgaaa taaccagggt
     2581 ttttggacgc cccttgttta tcagttccag cggatcagaa aattgggctg gagccttttg
     2641 atcaaacgtg agaacgtctt cttttttgtg accgctcgcg agcagtaaac gctctgcgct
     2701 ctgtccactt tttgccatac ctatgattgc tacgggtgtt tgaatcatgc gcagataatg
     2761 acttaaaatt ggttctttgc aaaggattga ttctgagtca aaatcaaaaa atgaatattc
     2821 agaaatccac atgctttaaa aacgccaaat acacagcagt cgtcagcgac ctgcatcttt
     2881 gcgaagcgga accagtcaat tctaagttcc ctttatggaa gaagtttaag accaagcaat
     2941 ttttctttga caatattttc acgacgttct tacaagaaat cgaaagcaga gccaaaggag
     3001 aaagtgttga actaatcctt aatggagata ttttcgattt cgatagtgtc ttgcgtcttc
     3061 cccaagagcc aatctttagg attagtcttt tagaaaaaag atgcggtctg tttccacgcg
     3121 aggaacgttc ttgttataaa ataaaagtga ttctggaaga ccacgcccct ttttttcagg
     3181 cacttcacag cttcgtttgc cgtggtcatc gcgtggtttt tgtcataggc aaccatgatt
     3241 tagagttaca ctttccagag gtccaaaaag aaatacgtca tgccttagat ctcacggaag
     3301 aacagcagaa ccaggttcgc tttgttgaat ggttttatat ttctaatgaa gatactttga
     3361 ttgaacacgg aaaccagtat gatccctatt gcctctgtga ggatcccatc aatccttatg
     3421 ttcggggtta taattacgtt tctttaaaac tgcccttcgg aaatttagct tgccgttata
     3481 ttatgaatgg tctgggattt ttcaatcccc acgtggattc caattatatt atgactttaa
     3541 aggaatacgt gactaccttt ttaaggtata tggcgcgggc tcaacccttt ctgatcttta
     3601 cttggctatc cggctccgtg gtcaccttac tctactcttt taaagatcgc ttggctgctc
     3661 cgattcgcaa tcctctaaaa attgaggaaa ggattgagca aatcgctgaa aaagccaatg
     3721 ctgaaccgcg gatggtccga gagcttaagg aactttttgt ggctcctgcg gcgagcaatc
     3781 catttttact ggcccgtgaa ttatggctgg accgagcctt tatgataggc attgccttct
     3841 ttttgatttt cgagcttttt atttttattc gtcaggtcta tgatatttca tttttttggg
     3901 cttttattcc cttatttttt atgttgccct tctttttatt ctatagtcag tccatcacct
     3961 caatggtttc tagttataag gaaccaaatg agagagtttt agccatgacc agtgctatca
     4021 ctcgagtgaa gcggatcatt tatggtcata cccactctgt tcgtcacgaa atgatcggag
     4081 ccgtggagca tttgaactca ggttgttggt cacccgcctt tttggatatt gaatgtacga
     4141 aacctattga tcagaagact tatgtttggc tttcacccca agaatcggga gaacgtcagg
     4201 ctgagcttta taagtttata gaggggcgat cccatttgtt tatgtctaca agcaatcgct
     4261 aggtgaaaat attgcagtta tgcggcgcat gacaaataaa atttgtcatc aaccaggggc
     4321 tatgcaaacc tcttagtgtt ttaaaagcat aaacggaggc taatactatg tcagaagtac
     4381 tagttgtaac tagcaaagta aaaaaaataa tcaaagaaaa aggcggaatg aatacttctg
     4441 ctgaaacgat cgacgtttta agtaaagcta ttgagcgact ctgcttaaaa ggcgtggata
     4501 gcgcgaaagc cgatggtcgt aaaaccgtta tggctcgcga tattgtcatc gatcatttat
     4561 agaaagtttc cgcttaattt gaaattaaaa agaagttcgc caggacttct ttttgtactt
     4621 ttttattcac ggttttatgg ttggtttaaa actgtgagtg cgtagctgat aaacatcata
     4681 caaagcatgc caattaaaaa tatccaagca atcatgcgac ggttaaaatt ggttttctct
     4741 gcacgggctt taacgaaaac attttcgcgc tcatcgttat cgagttctgg tggggattcc
     4801 cccgagaatt ctggcagatc aattggaaat cccaattcct tcagatgttc ttccatgcga
     4861 ggaagatctt tcgtggcgag tgtgatatag aaaatttgtc ctaaatactg ctcattgcga
     4921 taactgatct ggtttacgat attggagaag ttattgctct tgaatttctt ttcagcctct
     4981 tcatctttct ggatctcaaa aggaatattt cgaagttgaa gctcgtgaac tagcttatca
     5041 agctcttgtg gagtgagggg accatattga acaattttat ctgacatagt taaaccatcc
     5101 cctgaagttc ttcccatgaa ataactttca ccccaagtcc ttgagcttta tcgagcttag
     5161 aaccagggtc atctcctacg acaagataat ttaatttgga ggaaactgaa cctagaattt
     5221 tcccaccatt tttctcgata aagtctttag catcatctcg ctttactgga agagtacccg
     5281 taatgagaaa actttttccc gcaagcatgc ctgtagtgga tcgttgagga ctgcggatgc
     5341 tgactccaaa taaaaccaga tcttttacct ctttgacaag ttttggattg cttgtccatt
     5401 ccaaaatgcc ttttgcaacc ttggggccaa tttcgggaat caatagaaga tcgttctctt
     5461 tagcctggag aaagttttca atagttccga aatgttcagc taagtgccga gcggtttgtt
     5521 ctccaacaaa gcggatgcct agggagaaga taaatcgtgc taaagttgta ttcttactgg
     5581 cttcaatgct ggacagtata ttttcaacgg atttttctcc ttggcgctct aagggaagta
     5641 gattttcttt tttcaggcga taaagatcac taaatttgcg aacaagcttt tgatccacta
     5701 atgtttcaat gaggcggtct ccgattttat ctaaattcat cgctctgcgt gaagcaaagt
     5761 gtttcaaaga ttctttgatg atggcaatgc aaagaggatt cgtacagcga gtaacgacct
     5821 ccccttccag cttatgaacc tctgtcccac aagcggggca tttgttgggg agtaaaaatg
     5881 gctcggattt ttttggtcgt tttgcaagat ctacgtttac gatttcagga ataacatcgc
     5941 ctgcgcgttg aataataact gtatccccga ggcgaatgtc cttacggtca atttcctctt
     6001 ggttatgaag agttgcattt gtcactgtaa cacccccgac tcgaacgggc ttcataatcg
     6061 ctacaggagt gagggcacct gttcttccta cttgaatcac aatattttcc acaattgttt
     6121 gggcttgttc aggcttaaat ttagctgctg ttgcccagcg aggactgcga gccacaagtc
     6181 ccagatcctc ttgctgtcgc aaagcattta ccttaatgac aataccatca atgtcaaaag
     6241 gcagatgagg tctctctttt tcaatgaaat gataataatc aatagcttct tttgggctct
     6301 tgcaaattcg catgagttcc ggatgagaac ctgtcgccgt agggatagct accttttgaa
     6361 aataagtccc gatatctgct tggttttcaa attttatgcc gtcaacttcc cccagggcat
     6421 aggcaaaaaa ttttaaacgc cgtgagcctg caattttagg atccaattgt cgtatagtgc
     6481 cagcagcagc attgcgggga ttggcaaaac tgggttgccc attttcctgt tgctgttcat
     6541 tgagttttaa aaaatccgct ttcaaaataa gaacctcacc acgcacctct agaagctcag
     6601 ggggcctgga agtttctaat ttcagcggaa tggatttgat ggttctgata ttttgagtga
     6661 catcttcgcc tgtatagcca tccccgcgag taatcgcgcg tatcagaaaa cccttttcat
     6721 aaacaagttc catcgaaagc ccgtcaaact taggctcgac gtaatattct actacaggtc
     6781 cttcgcgctt aagaaatttt ttaacacgtt cgtcaaattc caaaatatct tctggtgaat
     6841 aactattggc taatgagagc atcggtttcc ggtgcgtgac tttctcgaaa gcccctaagg
     6901 gagccccgcc cacgcgctgt gaaggggagt cgctcaggtc aaggtgaccc agaagagcct
     6961 cgtttttttc gagttctaaa agttcagcga acagctgatc gtattcataa tccgaaataa
     7021 ggggtttgtc ggtgacataa tagttataat catgttcggc gatgatcttt ttgagctctt
     7081 catggcgttt tttggacatg ggtggacttg tatcatgggc tgtttgactg tgaaagaaat
     7141 tttgattagt ttatgagact tgcacccgcc taaggagttc cccatgattg ataaaaaaac
     7201 cattgagcat attgccaaat tagctcgtct tgaaatcact gaagatgagg ccaccgaata
     7261 cagccaacaa ctgggcaaag ccatggctca ctttgagcag atttcaaaag ttgacacgac
     7321 aggtattgaa cctctcgtaa cgccagccga gatcgaagat ttttggcggg aagatttggt
     7381 taaacaagaa ttcactcctg aagacatggt cagcaatgca ccatctaaag ccggtcattt
     7441 gtttaaagta ccacctgttg tataaggaat ataaaatgga tttaattcaa gcctcgatag
     7501 tagacatcgc agacgccgta aaggcaaaaa aagtcagtgc gaaagaaata acgactcaat
     7561 tcatcaaacg tgctgaaagc attaatccca agttaaacgc ctatacaagt tttaataatg
     7621 gcgctcttca agatgcggag gccattgatg cccggattgc taaaggagaa gatgcaggtg
     7681 ttttagcg
//
LOCUS       DATBTE010000411         4809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1150025, whole genome shotgun sequence.
ACCESSION   DATBTE010000411 DATBTE010000000
VERSION     DATBTE010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150025"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VIG33_15895"
     CDS             <1..1015
                     /locus_tag="VIG33_15895"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY8272374.1"
                     /translation="DHVKSYTALRSVAMSRDRFLLNGCPYYMRLVLDQGYWPDTYMTP
                     PSEEAIRKDIELTKAAGFNGVRKHQKVEDPNYLYWADVMGLMVWCEMPSAYRFTHESV
                     ERLMREWSEVIDRDMSHPCIVVWVPFNESWGVPDLAEKAAHQHCVQALYHLTKTLDPT
                     RPCIGNDGWESTATDIIGIHDYDSKPERLLKKYTLEASVADILSRYRPGGRVITVEGF
                     PHGGQPVMLTEFGGISFVEHTQFSNSWGYSTSFNAEDFRDQYERLVASVHRIELFAGF
                     CYTQLTDTFQETNGLFKADRTPKFSINSMARATRGVEDWRGELTSMPQPPPLPPPERE
                     ENP"
     gene            complement(1063..1557)
                     /locus_tag="VIG33_15900"
     CDS             complement(1063..1557)
                     /locus_tag="VIG33_15900"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY8272375.1"
                     /translation="MQAKINSEQGTQDKNFNFNLRWDHGAVISIGPNASLEEAAQLMK
                     EHQVGNVLVMDSNAVKSEVQGIVTDRDIALSLAGEEDIQDLRVVDIMSENVVCASEND
                     DFFKLVNLMSESGVTRLPLKSADGKVVGVVNAKNLLEILTKSLFELTKISEKQQENEQ
                     IHQH"
     gene            1724..3451
                     /locus_tag="VIG33_15905"
     CDS             1724..3451
                     /locus_tag="VIG33_15905"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY8272376.1"
                     /translation="MGLLNFFFILIFYSSQAAETRLCEHIFLKEGKLILNKNEQVLIC
                     GSNKAGQPWQSIPLPQAELHLKSILQNIGYLHPRFQREKEQLLVWTGPESEIKELQVE
                     QPVTILNPAKKRKVVGEALTSEKLNEVESWANLGFRSQGYACPEIQIEAQAWNETVRV
                     KTKLDSQKKIDLLIAQNLEGLNLDVLDRYRPFEKGDLYDIRETQIMVSRLIDDGLFQS
                     AFFEIACGQNEVALDLKTTIGKPKILRFGIGASTEELPFADLTFRNTRLDNKASSVTM
                     NLHISPRLQRFLVDSQLYWFPGWHRTFFGPRFRMTREIESAYQTDSGRLGADLGRRWD
                     MWKTRFVGKGGPTLNWVKTSRGRGPEDARYSSLEASLALMNHLYESSIWQQEQGWTAS
                     IFFRGQAQGLGSRINVNHYEVDYKYLWNIGHYAPPLWVLGTRIEGITVDASEVGNQDP
                     TPLIPIEDRVFAGGDQNLRGFPRKSINNQGLGYLSFLYLGFELRLIEELPYHLQPFLL
                     WDIGQVGKRRYTVDPPIFTSEGIGLRWLSPFGTVRSSLAQGRIFNRNGLDQPYPEQWV
                     FFFSFGQEF"
     gene            3451..>4809
                     /locus_tag="VIG33_15910"
     CDS             3451..>4809
                     /locus_tag="VIG33_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272377.1"
                     /translation="MSGILKKIFKFFMFFVLTLIVLTAGLIAWVMINPKSVWVIVEKH
                     FLPPDLKVTWEEIDFRSTHLSGLSFNFALEFHELQIKKAQPPLDFPIHFLRLEASIDP
                     RNSEHKVVIHNWQLRAEKSLRFKWISKNSGKTERNPFQYLQDSLGHVKSLRRLIKLEN
                     LEIDVKQFLLELTGGKIFPLSIEAMQNQGRTPNLLKYKISVKIPGQQTTNINSRGQFS
                     LNFLDDDKFQLNGSANLSRHDIKATQTFKVAYSKDTANIYLSGFLLIPRKTLRITARP
                     SAHMKLTAKGAEIDLNIVSLKGLPGELMKIDSAQVHLSSPFDKGIFWSKRPTTLSLSC
                     PIKIFFIDKDMRPPLEKACQCKIPELWMAHVEGQAWVENLLSLPAHAVPALDLRLRLE
                     DVDNKLLSLNSAAQLQVTKELNKFSMAPQIDAQAVIHSFQGLRNFLDAKGVLIPAPLS
                     VLD"
BASE COUNT         1321 a         1062 c         1025 g         1401 t
ORIGIN      
        1 tgatcatgtt aaatcctata ccgcacttcg cagcgttgct atgagcagag accgattttt
       61 attgaatgga tgtccttatt acatgcgtct ggttttggac cagggttact ggcctgacac
      121 ctacatgact cctccttctg aagaggccat tcgtaaagat atagaactca cgaaagcggc
      181 gggatttaac ggtgtacgta agcatcagaa agttgaagat cctaattatt tatattgggc
      241 tgatgtcatg ggactcatgg tttggtgtga aatgccaagt gcttatcgtt tcactcatga
      301 atccgttgag cgcttgatgc gggagtggtc tgaagtcatc gatcgcgata tgagtcatcc
      361 gtgcattgtg gtgtgggtgc cctttaacga atcctggggc gtacctgatc tagcggagaa
      421 agcggcccat cagcactgtg tgcaggctct ttatcatctt actaaaactt tggatccgac
      481 acgaccttgt attggcaatg atggctggga aagtacggcg acagatatca taggcattca
      541 tgattacgat tcaaaacctg agagacttct aaaaaaatat actctggaag caagcgtcgc
      601 ggatatcttg agtcgctacc gtccaggcgg aagagttatt acggtagaag gctttcccca
      661 tggtggtcaa cctgtgatgt taacggagtt tggcggaatt tcttttgtgg agcataccca
      721 atttagcaac tcttggggtt attccaccag ctttaacgcg gaagatttca gggatcaata
      781 tgaaaggctt gtagcctccg tccatcgcat cgagctgttc gctggttttt gttacactca
      841 gttgactgac acttttcaag agaccaacgg tctttttaag gctgaccgga ctccaaagtt
      901 ttccatcaac tccatggcgc gagccacgcg tggggtggaa gattggcgag gcgagcttac
      961 ttctatgcca cagccccctc ctctgccgcc acctgagcgg gaggagaatc cgtaaaaaat
     1021 aaacaggtca gggttccagc agctgaaacc tgacctgtaa ttttagtgtt gatgaatttg
     1081 ctcgttttct tgttgttttt cagaaatctt agtgagttca aatagactct tcgtcaagat
     1141 ctctaaaaga ttctttgcat tgactactcc gacgactttt ccatcagcac tcttaagggg
     1201 cagtctcgtg actccggact cgctcatcaa attaaccaat ttaaaaaagt catcattttc
     1261 agaggcgcat acaacattct ctgacatgat gtccaccact ctcaggtctt ggatgtcttc
     1321 ttctcctgcc aacgacaatg caatatcccg atcggttaca attccttgca cttcactttt
     1381 taccgcattg ctatccatca ccagaacatt ccctacctga tgttctttca ttaattgagc
     1441 ggcctcttca agggaagcat tgggcccaat cgagatcaca gctccatggt cccatcttaa
     1501 attgaagtta aagtttttat cctgagtgcc ttgttcagag tttatttttg cctgcataaa
     1561 aatttccttt cgttactatg aaagtaaaaa agtattcgct attagtgagt ttgaaaagat
     1621 acctgtgcag acatacatgt ttttagttcg ctttgtggct tggcataaag gttagattcc
     1681 actcactagc tgagtcgaag tctcatatat aataggcccc cgcatgggtt tattaaattt
     1741 tttcttcatt ttaatttttt attccagcca agctgctgaa accagactct gcgaacatat
     1801 ttttctcaaa gaaggaaaac tcatccttaa caaaaatgag caggtcctta tttgcggttc
     1861 aaataaggca ggtcagccct ggcaaagcat tcccctcccg caggcggagc ttcatcttaa
     1921 atccatttta caaaatatcg gttatcttca tcctcgattt caaagagaaa aagagcaact
     1981 gctggtttgg acagggcccg aaagtgaaat caaagaactt caagtagaac agcccgtaac
     2041 catcttaaac ccagctaaaa aaagaaaagt tgtcggagaa gctttgacct cagaaaagct
     2101 caatgaggtt gaatcttggg ccaatttagg atttcgtagt cagggctatg cctgtccaga
     2161 aattcagata gaagcacaag cctggaacga aactgtaaga gtaaaaacaa agctcgattc
     2221 gcaaaaaaaa atagatcttc ttatcgcaca aaatttagag ggccttaacc tcgatgtact
     2281 cgatagatat cggcccttcg aaaaggggga tctctatgat atacgcgaaa ctcaaattat
     2341 ggtttctcga ttaatcgacg atggactttt ccaaagcgct ttttttgaaa tcgcttgcgg
     2401 tcaaaatgag gtcgcccttg atttgaaaac caccatcggc aaaccaaaga ttttaagatt
     2461 tggtatcggc gcctcaaccg aagaattgcc ttttgcggat ctgacttttc gcaatactcg
     2521 tcttgacaat aaagcctcct ctgtgaccat gaaccttcat atttctcctc gattgcagag
     2581 attcttagtg gattcacagc tctattggtt tcccgggtgg catagaactt tttttggccc
     2641 tcgctttcgc atgactcgag aaattgaatc tgcttatcag acggattctg ggcgcctcgg
     2701 cgccgacctc ggcagaaggt gggatatgtg gaaaacacgc tttgttggca aaggggggcc
     2761 gaccctcaat tgggtgaaaa ccagtcgcgg gcgaggaccg gaagatgcca gatattcctc
     2821 cctcgaggcg tcattagctc tcatgaacca tctttatgaa tcttccattt ggcaacaaga
     2881 acaaggctgg actgccagta ttttttttcg tggtcaggct caaggtttag gatcccgaat
     2941 aaatgtaaat cattatgaag tggattataa atatctctgg aatattggac actatgctcc
     3001 tccgttatgg gtgttaggaa ctcgcatcga aggaatcacg gtagatgcct ccgaagtcgg
     3061 aaatcaggat ccaacccctc tcattccaat tgaagatcgg gtctttgctg gcggcgatca
     3121 aaatttgcgg ggcttcccaa gaaaaagtat caacaaccaa ggtctcggct acttgagctt
     3181 tctttactta ggatttgaac tgcgactcat tgaagaattg ccttaccact tgcagccttt
     3241 tcttctctgg gacatcggcc aggtaggaaa gcgacgttac actgtggatc caccgatctt
     3301 tacttcagag gggattggtc tgagatggct ttcacccttt ggcacggtca gaagttcctt
     3361 agcacaaggc agaattttta atcgaaatgg cctggatcag ccttaccctg aacaatgggt
     3421 gttttttttt agctttggac aggagtttta atgtccggaa ttcttaaaaa gatttttaaa
     3481 ttttttatgt tcttcgtctt aactttgatc gttcttactg cgggattgat tgcctgggtg
     3541 atgataaatc ctaaatcagt atgggtgatt gtcgaaaagc attttctccc gccggacctc
     3601 aaagtgactt gggaagaaat agattttcgc tcaactcacc tttctggatt gagtttcaat
     3661 tttgccttgg agttccatga gcttcaaatc aaaaaagctc aacctcccct ggattttccc
     3721 attcattttt tgcgcttaga agcttccatt gaccctagaa attctgagca taaagtagtg
     3781 attcataatt ggcagcttcg cgccgaaaag agtttgcgct tcaaatggat ctcgaaaaat
     3841 agcggtaaaa ccgagagaaa cccttttcaa taccttcaag attctctcgg tcatgttaaa
     3901 agtttaagac gtcttatcaa attggaaaat ttagaaatag acgttaaaca attcctctta
     3961 gaattaactg gcggtaaaat ctttcctctt tcaatagagg ccatgcaaaa tcaaggacga
     4021 acacccaatc tattaaaata caaaatatct gttaaaatac ccggtcaaca aacgacgaat
     4081 ataaactcgc gaggtcagtt ctctcttaac ttccttgacg acgataagtt tcaattaaat
     4141 gggtccgcga atctctcacg ccatgatatc aaggcaaccc agacttttaa agtagcctac
     4201 agcaaggata cggctaatat ttatctgagt ggttttctgt taatccccag gaaaactcta
     4261 cgcataaccg ctcgtccttc cgctcacatg aagttgacag caaaaggtgc ggagattgat
     4321 ttaaatattg tttccttaaa gggtttaccg ggggagctga tgaagattga ttcagcccaa
     4381 gttcatctta gttctccttt tgataaaggc atcttctgga gtaaacgccc cacgacctta
     4441 tcgctttcct gtccaattaa aatatttttt attgataaag acatgaggcc acccctcgag
     4501 aaagcctgtc agtgtaaaat tccagaacta tggatggctc atgtcgaggg tcaagcctgg
     4561 gtggaaaact tactttctct tccagctcat gctgtccccg ccttggatct aagattacgc
     4621 cttgaagatg tggataataa actcctctca ttgaattcag cggctcaact tcaggttaca
     4681 aaagaactaa ataaattttc aatggcccct caaatagatg cccaagctgt aatccatagt
     4741 tttcaaggac tgcgtaattt tttagatgcc aagggagtgt tgattccagc gcccttgagt
     4801 gtgcttgac
//
LOCUS       DATBTE010000412         2678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1763950, whole genome shotgun sequence.
ACCESSION   DATBTE010000412 DATBTE010000000
VERSION     DATBTE010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763950"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..2077)
                     /locus_tag="VIG33_15915"
     CDS             complement(197..2077)
                     /locus_tag="VIG33_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272378.1"
                     /translation="MNKNPIEFVVIVQDLISRRPFFGISGLGLVNWKGKAMMARVLWF
                     IFPLAITYALKAPCAPPKNQALLGAATYITMPLRKLSHPTLVYVNKEYLQARGISLDQ
                     VLEKFGFASPVDGEFADVYLDNVKTAYVDGNGGIGLNENFGSGRAAIVDEEWQIKGSG
                     RTPMVNPNTDAYHRNGASFLPEAIHEAIWGNLLAEELPFGAYRVVAIIATGSMVASGE
                     QEPQPRFLIVREDPLRPGHFVINPTSEKMNDPRDRARVQEAMKHLVEALPQPAGPVPK
                     SEVARFRSGIFEFIDRQAIQHAYAWTHSLFHGGTSPTNAALDGRMLDFGTYSAFDGYP
                     RVRILDDDGFMGDTEVYKRDLLKDIRDSWLKTLPSHLLANLPSEKEWFERFETVFQKT
                     KKTEILRLAGALSEFKEELLKTQAGQNLAKTLLELAMAGNEKGIEAWRGEEPFGQGRY
                     NLGKILVAASSVNLQDFDAENKTLRTLIPEEADRKTFVDHYQAFFKLQRDLVLPLGIN
                     PEAEATYRKYSSQIRNKKMVTLFHKVDNEKHIWKILEDYQKDHDPRPTQKYIDAMISQ
                     NRREFPAAPYTIVLKETKSGEKIERDIFDARTNQFMKTQIQSQYGQGGAHFRCEAVFL
                     LK"
     gene            2276..>2678
                     /locus_tag="VIG33_15920"
     CDS             2276..>2678
                     /locus_tag="VIG33_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HEY8272379.1"
                     /translation="MDSQIHFPQETDSVEINSVLFNFRNIKTVIKDFYRKVAMDPVLS
                     VPFQSVEDWPHHVVRLTHFWWIRFGGDSYLNVSYNPPEKHFHAGFNQEFLTRWLGLFQ
                     ETLQDKLSLEQATLWGVIAERMGVALTFKNDL"
BASE COUNT          719 a          587 c          599 g          773 t
ORIGIN      
        1 gactaaagag cgcgccaaac actcctgata agcctaaaaa gaggctgctg gcgtgaaaat
       61 gcctgtcatc tgtttgaaaa ctagacactt taaacagcag taggtgtctc ttgactattc
      121 tcttacacat aacagtggat actgcaaccg tcgtaccaca ctctttcatc tcaagcgggg
      181 caaaaaatat attaatctat ttcaaaagaa agacagcttc acagcgaaag tgagcgcctc
      241 cctgtccata ttggctttga atttgagttt tcataaattg attggtcctt gcgtcgaaaa
      301 tatctctctc aattttttcg ccactttttg tttcctttag cacgatagtg taaggggccg
      361 ctggaaactc acgtctgttt tgagaaatca ttgcatcgat atatttttgt gtgggacggg
      421 ggtcatggtc tttctggtaa tcttcaagaa ttttccaaat atgcttttcg ttgtcgactt
      481 tatggaaaag ggtcaccatt tttttgtttc ggatttggct ggaatattta cgataagtgg
      541 cctcagcttc ggggttaatg ccaagaggga gtactaaatc tctttgcagc ttgaaaaagg
      601 cctgatagtg gtcaacaaac gtttttctgt cagcctcttc aggaatgaga gtccttaaag
      661 tcttattttc cgcatcgaag tcttgtaaat tcacactaga tgcagcaact aaaatttttc
      721 caagattgta tcgaccttga ccaaaaggct cttcacctct ccatgcttcg attccttttt
      781 cattaccggc catggccaac tccaggagag ttttcgctaa attttgacca gcttgagttt
      841 ttaatagctc ttctttgaat tcggaaaggg ctccggctag tctgaggatt tcagttttct
      901 tagtcttttg gaaaaccgtt tcgaagcgtt caaaccactc tttttctgag ggcaagttgg
      961 ctaacaaatg agatggcaac gttttaagcc aactgtcacg aatgtctttt aataaatcgc
     1021 gtttgtagac ctcagtgtct cccatgaaac catcatcatc taaaatgcga actctggggt
     1081 aaccatcaaa agccgaataa gttccaaaat ctaacatacg accatccaag gcggcgttgg
     1141 tggggcttgt tccaccgtgg aatagactat gggtccaggc ataggcatgt tgaatagctt
     1201 gccgatcgat gaattcaaaa atcccggagc ggaatcgcgc cacttcactt tttggaacag
     1261 gcccggctgg ttgcggtaag gcctctacaa gatgtttcat agcctcttga actcgagcgc
     1321 ggtcgcgtgg atcattcatt ttttcagagg tgggattgat gacaaagtgg cctggtcgca
     1381 gtggatcctc tcgaacaatc aaaaatcttg gttgcggttc ctgttctcca gaagcgacca
     1441 tagatccggt tgcgatgata gcgacaactc ggtaggctcc gaaaggaagt tcttcagcta
     1501 aaagattgcc ccagatcgcc tcatgaattg cctcgggtaa aaacgaagcg ccgttgcgat
     1561 gataggcatc cgtatttgga ttgaccatgg gggtgcgacc gcttccttta atctgccatt
     1621 cttcatctac aattgcggca cggccgctgc caaaattttc gtttaaacca atgccgccgt
     1681 tgccatccac ataggctgtt ttgacattat cgaggtaaac gtcagcaaac tcgccatcga
     1741 ccggggaggc aaaaccaaat ttctccagca cctgatccaa actgattccc cgggcttgaa
     1801 gatattcttt gtttacatat actaaagtag gatgagacag ctttcgaagc ggcatagtga
     1861 tatacgtggc tgcccccagg agtgcctgat ttttaggggg cgcacacgga gccttcaggg
     1921 cgtaagttat tgccaggggg aaaatgaacc atagaactcg agccatcata gccttacctt
     1981 tccaattaac aagacccaat ccagagatgc caaaaaatgg ccttctgctg attagatcct
     2041 gaacaatcac cacaaactca attgggtttt tgttcaaaag ttcctcttgg aggaggtcaa
     2101 tgcaaaagaa gtgcctgttc gaattgataa gtacatgctc agacctaggt ccttatgaga
     2161 atttcttaga tcttttctgg agggctcaaa taaaaattaa caatcatact taggaagtga
     2221 cctatgacag tgttgggggt ataggaatga acttgatacg catgaaggga attacatgga
     2281 ttcacaaatt cattttccgc aggaaacgga ttctgtcgag atcaactcgg tcctttttaa
     2341 ctttcggaat ataaagactg tcattaagga tttttacagg aaagtagcaa tggatccggt
     2401 attaagcgtt ccgtttcaat cagtggaaga ttggccgcat catgtggtgc gcttaactca
     2461 cttttggtgg attcgttttg gcggggactc ttacctgaac gtttcctaca atcccccgga
     2521 aaaacatttc catgccgggt tcaaccagga gttcctcact cgttggcttg gtttatttca
     2581 agaaactttg caagataaat taagcttgga gcaggctaca ctgtggggag ttatcgccga
     2641 aagaatgggc gtcgccctca cttttaaaaa tgatcttt
//
LOCUS       DATBTE010000413         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_809209, whole genome shotgun sequence.
ACCESSION   DATBTE010000413 DATBTE010000000
VERSION     DATBTE010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809209"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VIG33_15925"
     CDS             <1..232
                     /locus_tag="VIG33_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272380.1"
                     /translation="NSNISTILPPASKTLIPIKFQAEYADMGLILNDFLLKDELNTLF
                     ESEILLSFPNTLSLPSRQSLKIEKKEKIKLRE"
     gene            complement(242..2932)
                     /gene="ppc"
                     /locus_tag="VIG33_15930"
     CDS             complement(242..2932)
                     /gene="ppc"
                     /locus_tag="VIG33_15930"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEY8272381.1"
                     /translation="MSELNDGSVMKDPLFFLKEDIHLLGDLLGEVLKEQLGLDFFESV
                     ETVRKLSKSGRAGKDKDFYHLLDLLRNLSAEQMIPLSRAFSQFLSLANFAENHHRIRR
                     RRDYELKAGASAQRGSTEDVVSELLRQGQSPELILQTVKDMRIQLVLTAHPTEINRRT
                     LQSKYLNIAKSLNTKDRKDLTPREKRELRIALKREIMAAWMTSEIHEQRPTPVEEARG
                     GLVIFETVLWNALPNYLRIMDEVLRKHLGHSLPLESCPIRFDSWMGGDRDGNPSVTPE
                     MTRKTVYMTRWLAAELYYVELEALVKDLSMQESSAELKAVVGSRLEPYRALLRPLRER
                     MRATGFYYYELYNKRVHPGKRSGVGEEKVFTEAKELLEPLMLAYHSLIKTGAGIIAEG
                     RLADVIRRVHVFGLTLVRLDVRQDSSRHASLLRMILQARGVGDYQSFSEKQKQEFLVA
                     DLQNSQAVIPESLFLNDEDADTLSTFQVLADLPADSMGAYVISMAREPSDILAVEWLQ
                     KACAVKVPLRVVPLFEQVEDLRNSGRTLEALFSVPWYLQRIKGQQEIMIGYSDSAKTA
                     GRLTASWELYQAQEVITEVCTRYGVHGTLFHGRGGSVSRGGGPTYKAIAAQPPGSIQG
                     TMRVTEQGEMILTKFGLPELAIRNLELYSSAVLEATLRPALTPTPAWRKHMQELSESA
                     EISYRQVIEHTPEFIDYFQAATPERELAFLKVGSRPPRRKGTGAIKSLRAIPWSFAWT
                     QTRLHLPTWLGMEKAFEKSIEKGALEEIRTMYEQWPFFRSTIDLVEMVLAKSDSRIAA
                     LYDEALVPNSLKPIGEEMRFRLQKITDFVLQINRHSTLVEDNPTLKRSIQVRNPYVDP
                     INLMQIELLRRLRKDPNSEIFKKALVITINGIAAGMRNTG"
     gene            3076..3393
                     /locus_tag="VIG33_15935"
     CDS             3076..3393
                     /locus_tag="VIG33_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272382.1"
                     /translation="MASPEKYRRLVKAIQGFDNFNFFVDLIVLSLFATISIAVANKFL
                     PKKLLDMDWFDLSWRTIGACFFIFLVLLITQLRKERKRRILSAFEQEAAIKAARKNNF
                     RKY"
BASE COUNT         1044 a          886 c          715 g          956 t
ORIGIN      
        1 aaatagcaat atttcaacaa tacttcctcc cgccagcaaa accttgattc ctataaaatt
       61 tcaagctgaa tatgcggata tggggctgat cctgaatgac tttttattaa aggacgagct
      121 caatacactt tttgaatcag aaatattatt aagctttccg aacaccctga gtctgccctc
      181 tcggcaatcc ctcaaaatcg agaaaaaaga aaaaattaaa ttacgagagt gattaatttt
      241 attaaccggt gttgcgcatg cctgcggcaa tgccattgat cgtaataacc agtgctttct
      301 taaagatttc cgagtttgga tcttttctca gacgacggag aagttcaatt tgcatcaaat
      361 taatgggatc gacataggga ttgcgaactt gaatggatct cttcaacgta gggttgtctt
      421 caactaaagt cgaatgccgg ttaatttgca aaacaaaatc cgtgattttt tgcaatcgaa
      481 acctcatttc ttccccaatc ggcttcaagg agttgggaac taaagcttca tcgtacaggg
      541 cagcaatgcg cgagtccgac tttgccagaa ccatttccac taaatcaatt gtggaacgaa
      601 aaaaaggcca ctgctcatac atcgtgcgaa tttcttccaa agcacctttc tcgatgcttt
      661 tttcaaatgc cttctccatc cctaaccaag tcggaagatg aagcctggtc tgcgtccagg
      721 caaaagacca tggaatcgcc cgtaaagatt taatcgcgcc agtgccttta cgccgtggcg
      781 gtcgactgcc aaccttaaga aaagcaagct cccgctctgg cgtggccgcc tgaaagtagt
      841 caataaattc cggcgtgtgc tcgatgactt ggcggtaaga aatctcagcg ctttcggaga
      901 gttcttgcat gtgttttcgc caagccggag tcggcgtaag agccggccgc agcgtcgcct
      961 ctaaaactgc tgaagaatag agttccagat ttcgaatagc caactcaggc aatccaaact
     1021 tcgttagaat catctctcct tgttcagtga cccgcatagt gccctgaata ctaccgggag
     1081 gctgcgccgc aatcgcctta taggttggac cacccccgcg actgacactg ccaccacgac
     1141 cgtgaaaaag cgtgccgtgc acaccgtagc gcgtgcacac ctcagtgatt acttcctggg
     1201 cttgatacaa ttcccaggag gcggtcaagc ggccggcggt tttcgcagaa tccgaatagc
     1261 caatcatgat ctcttgttga cctttgattc tctgtaagta ccaaggaact gaaaataaag
     1321 cctccagcgt tcgaccggaa tttcgcagat cctccacttg ttcaaacaaa ggaacgactc
     1381 gcaaaggcac tttcaccgca caggctttct gcaaccactc aaccgccaaa atatccgaag
     1441 gctcccgggc catcgaaatc acataagctc ccatggaatc cgcaggtaaa tccgccaata
     1501 cctggaatgt gctaagtgta tcggcgtctt catcatttaa aaacaaagac tctggaatca
     1561 ccgcttgaga attttgcaag tcggcgacta aaaactcttg cttttgtttt tcgctaaaac
     1621 tttgatagtc gcccacacca cgagcctgga gaatcattcg caacagggaa gcgtggcgcg
     1681 aagagtcctg acgcacatcc aagcgcacca gcgtaagacc aaagacatgc actctgcgaa
     1741 tgacatccgc caaacgcccc tcagcgataa ttccggcacc agttttaatt aaagaatgat
     1801 atgccaacat cagcggctct aatagctctt tggcttccgt aaaaactttt tcttctccaa
     1861 cgcctgaccg cttaccagga tgtacgcgtt tattgtaaag ttcataataa taaaagcccg
     1921 tggctcgcat ccgctcccgc aaaggacgta acaaagcacg atagggttct aaacgggaac
     1981 ccaccactgc ttttagttcc gcactgctct cttgcatcga aaggtctttc accaaagctt
     2041 ctaattccac atagtataac tcagccgcaa gccatcgtgt catgtacact gttttacgag
     2101 tcatctccgg cgtcaccgag ggatttccat cacgatctcc tcccatccac gaatcaaaac
     2161 ggatgggaca agactctagc ggaagcgagt gaccaaggtg cttgcgcaat acttcatcca
     2221 ttatacgcaa ataattaggc agagcattcc agagaactgt ctcgaaaata actaggccac
     2281 ctcgagcctc ctccacgggt gtagggcgct gttcatggat ttcactggtc atccacgctg
     2341 ccataatttc tcgttttaaa gcaatccgaa gctcacgttt ttctcgcgga gtgagatctt
     2401 tacgatcttt cgtattgaga ctcttggcga tgttcaaata tttactctgc aaagtacgac
     2461 ggttgatctc ggtgggatga gccgttagca cgagttgaat acgcatgtcc ttaacagttt
     2521 gaagaattaa ttcaggactt tgcccttgac gaagaagttc actgacaacg tcctcagtag
     2581 agccgcgttg agcgctcgcg cccgccttta attcataatc ccttcgacga cggatacgat
     2641 gatgattctc cgcaaagttg gccaaagata aaaactgaga aaaggcccgt gatagaggaa
     2701 tcatttgctc agcacttaaa tttctcagca agtccaggag atgataaaaa tctttatctt
     2761 tccctgcacg gccactttta gaaagctttc gtacagtttc cacactttca aaaaaatcaa
     2821 gacctagctg ctcttttaaa acttcgccga gaagatctcc taacaagtga atatcttctt
     2881 ttaagaaaaa aagaggatct ttcatcacgc tcccatcatt taactcactc aaccctagcc
     2941 ccccttgctt aaaagtcaag ttgaaggtca aggtgaatga gctgaaaaac agggatcttt
     3001 gacacaactc tctgttcaga atatcctgaa aaaggttaac gaccaaataa ctctttaaat
     3061 tgaaaggcga agataatggc atcccccgaa aaatatcgac gtctagtaaa agcaatacaa
     3121 ggttttgaca attttaattt ttttgtagat ctcattgtcc tctctttatt tgcgactatc
     3181 tccatcgctg tcgctaataa attccttcct aaaaaacttt tagatatgga ctggttcgat
     3241 ctatcatggc gaacaattgg ggcttgtttc tttatatttc tagttctttt aattactcaa
     3301 ctccgtaagg agcgaaaaag aagaatcctg tctgcctttg aacaagaagc ggctatcaaa
     3361 gcggctcgaa aaaacaattt caggaaatat taaactgaaa tacaaaacta atttatattt
     3421 atataattcg ccgttcaaaa aaaataaacc aagactttat cgagaaccta tggcttttcc
     3481 gtcccttttg ttagcactat cgtccagaac ttagccttta ggctatcagc ctcagccctt
     3541 tttgatgtct aatgttatat tataaaggag gtctgaatca tgagtttaaa tagaaattat
     3601 t
//
LOCUS       DATBTE010000414         5220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2449576, whole genome shotgun sequence.
ACCESSION   DATBTE010000414 DATBTE010000000
VERSION     DATBTE010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2449576"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1293)
                     /gene="gshA"
                     /locus_tag="VIG33_15940"
     CDS             complement(25..1293)
                     /gene="gshA"
                     /locus_tag="VIG33_15940"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY8272383.1"
                     /translation="MVKQLLHRQILEHKDAICKWFAAKTSGVAYPIYSSYDIRDAGYK
                     VSNVDANIFPAGFNNICPVDKETSVGLMATYIDKHYGRSVRNILLVTEEHTNNAFYWE
                     NVYTIQSLIEQAGKTVRIAIPRDLPEPLKILSSAGRELTVNSALKEGSLLKEFKPDLV
                     ISNNDFSEAYEEWAKTLDFAINPPREFGWYQRKKSRYFKFYNELVNEFAEVAKFDPFL
                     LRVETEIYSHFDINSDESRNDLADRVDQMLARLKVEYQKRGIMQEPFVFVKNNAGTYG
                     LAVIRVGSGEEVRQWNYKSRKKMKAAKGSRDVEEVIIQEGIPSIVQADNASAEPVIYM
                     IGCELAGGFLRTHSEKSSTESLNSPGAVYKRLCVSDLSVRAEGCPQENVYGWSAKLGL
                     LAIALEGQEMGVEFKNYRTGSCNQGPSRSS"
     gene            complement(1307..2407)
                     /locus_tag="VIG33_15945"
     CDS             complement(1307..2407)
                     /locus_tag="VIG33_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8272384.1"
                     /translation="MRLWIILSVIFLFAGCSLFPVFHKKQQPTQAFILEARENDDFQL
                     IEQKLKEQNFEAALQSIETFQTAYPYSLKLQRARFMKAGALEELGRWTEAADTYKAIS
                     TISGKNQPEISAKSLYRLSYVYEALGDNQRVITVLVEAAKYHKYLPPEVINAEIPSRL
                     AMIYAKENNSKEAQKWLSEADKGLKRTLESRNEPLTDKWLAELYFNMGSISTQQLSND
                     NIITIIQGQSAVQKYLIEALQYNDSVWSAKALKSIRATYLDLWKAIETLPEEPGYEPL
                     VAQKMKKDEQLRLMGSFSDLIKEAELYRPGREQTTNPYQSEFFNFLEELQTRVHAILG
                     EALYTPLMSGRAKAAKKPKAPVKIVPSEDPNL"
     gene            complement(2410..3468)
                     /locus_tag="VIG33_15950"
     CDS             complement(2410..3468)
                     /locus_tag="VIG33_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEY8272385.1"
                     /translation="MKILVCVGNGGVGKTTLAAAMGVRAAQQGLKVLVLTIDPAKRLA
                     TTLGIEGKKDITRVPGQNFKGELFASIVDHKQTFDDFVLRAAGKSESAKKLFQNRLYQ
                     QLSTSLSGSQDFTALEKLYSCFESKEFDLIILDTPPSKHAIDFLNAPQKLAVLFNDKV
                     ARWFRESKNKSVGGTFLNILNTGTKQVLNIMESVTGSELLRELSDFFMSIEQWQEHLE
                     ARIINVHRMMVSSDTQFCLVTSFDQAKLLEAEYFLREIRKGGYNLRSVILNRSYPEWF
                     SDKENPLYEAPAGSEKIFTLYQQEKAFYSQRDREHKKFAAHISKSVQVLRLPELSDPI
                     SDLHGLIKVAEILKEKEG"
     gene            complement(3465..4337)
                     /locus_tag="VIG33_15955"
     CDS             complement(3465..4337)
                     /locus_tag="VIG33_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEY8272386.1"
                     /translation="MKSEIIFVTGKGGVGKSTVAAALALKKAQAGQRTLLVEIGDQSF
                     YQDYFQLPKVGFEPVKVKENFSVALWTGQNCLREYIIHLIKVETLYRLFFENAVMKAF
                     INVAPALPELAIMGKATSGPRHHGPPLSYDCLVVDCYATGHFMALMGAAEGMAMAVKF
                     GPMGEQSRGIDKVLRDPSLCQYYLVCLPEELPVRESEELYQQLKDHYGIQAKLVLNKF
                     LEEAIPLKEYVSAQTSSFEEFRAFAEDDQRNRHYHHEMKIRLQRMDTQVQCLPTIWDH
                     EVEKVLAQISSRLS"
     gene            4466..>5220
                     /gene="tig"
                     /locus_tag="VIG33_15960"
     CDS             4466..>5220
                     /gene="tig"
                     /locus_tag="VIG33_15960"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY8272387.1"
                     /translation="MKTNMEKISSLHHKINVQVPVTVVQTAFNRIFNSIQKEVSIKGF
                     RKGKAPLTTIRSMYNDRIKQDVAQELIQMHYIKALTEHKLEPISNPEFEFDIPAETKE
                     FSFSATFDVRPEVNLKKYEGFEVEKEKFTFDDKKIDEVLQNIRNSRASFEDVLEVRPA
                     QMGDVAIIDFAGFVDGKPLENGTGTDHNLDLGTKQFIEGFEEGIVGMNVGDSKTLNLK
                     FPNPYHSVELAGKAVEFKVTLKGMKKKVLPELN"
BASE COUNT         1425 a         1206 c         1091 g         1498 t
ORIGIN      
        1 tttagtatta tgtttaagct tttatcagga cgatcggctt ggaccttgat tacacgaacc
       61 agttctataa ttcttaaact ccacacccat ctcttggcct tcgagagcta tggcgagaag
      121 ccccagctta gcactccaac catagacatt ttcttgaggg cagccctcgg cacgaacgga
      181 aagatcagag acgcacaggc gtttgtaaac agctcccggg ctattcaggc tttcggtcga
      241 gcttttttca gaatgggtcc ttaagaaccc tcctgcgagc tcacacccaa tcatataaat
      301 gaccggttca gctgaagcat tgtccgcttg tacaatggac ggaatgcctt cctgaataat
      361 gacttcttca acgtcacgac tgccttttgc ggctttcatt tttttacggg atttgtaatt
      421 ccattggcga acttcttctc cagatcccac tcgaatcacc gctaaaccat aagtccccgc
      481 attgtttttt acaaatacga agggctcttg catgataccg cgcttttggt attcgacctt
      541 taggcgtgct aacatctgat ccacccggtc tgccaaatca ttgcggcttt cgtcgctgtt
      601 gatgtcgaag tgagaatata tttcggtttc caccctcaat aagaaagggt caaatttcgc
      661 cacttccgca aattcgttga cgagttcatt ataaaattta aaataacggc ttttcttgcg
      721 ctgataccaa ccaaactcac gcggtggatt tattgcgaaa tccagggttt tcgcccattc
      781 ctcgtaggct tcagaaaaat cattattact gatgaccaaa tcgggcttga actcttttaa
      841 cagcgatccc tctttcaaag cggaattcac cgttaattca cgtccggcag aacttagaat
      901 ttttaatggc tctggaaggt cacgcggaat tgcaatgcgc acggttttac cggcctgctc
      961 gatcaaagat tggatcgtat atacattttc ccagtagaag gcattgttgg tgtgttcttc
     1021 ggtaaccaaa agaatatttc ttacgctccg gccataatgc ttatcaatat aggtggccat
     1081 taaaccaact gaagtttcct tgtctacggg acagatattg ttaaaccctg ctggaaaaat
     1141 attggcatcc acattagaaa ctttgtagcc ggcatcacgg atatcgtagc tggaataaat
     1201 cggataggcc acgccactag tctttgccgc aaaccatttg cagatggcat ccttatgttc
     1261 gagtatctgt ctgtgcaata gttgttttac catgagaggg cttctcctat aaatttggat
     1321 cttcagaagg aacaattttg accggagctt tgggtttctt agcagcttta gctcggccgg
     1381 acatcagcgg agtataaagg gcctccccta aaatcgcgtg aacccgggtt tgaagctctt
     1441 ccaaaaaatt aaaaaattca ctttgatacg gattggtggt ttgttctctg cctgggcgat
     1501 agagttccgc ttctttgata agatcagaga aagatcccat taatcgaagc tgctcatctt
     1561 ttttcatttt ttgcgcaact agaggctcat agcccggctc ttctggtaag gtttcaatcg
     1621 ctttccatag atccaggtaa gttgcacgga tacttttaag cgctttcgcc gaccacacag
     1681 aatcattgta ttgcaatgcc tcgatcaaat atttttgcac cgccgactgt ccttgaataa
     1741 tggttatgat gttgtcgttt gacaattgct gagtcgaaat tgaacccata ttaaaataaa
     1801 gttcagccaa ccacttatct gttaaaggct cattgcgtga ctcaagagtt ctttttaatc
     1861 ctttatcggc ttcgctcaac catttctgcg cttctttaga attattttct tttgcataga
     1921 tcatggccaa acgcgaagga atctcggcgt tgatcacttc cggtggaaga tatttgtgat
     1981 atttagccgc ttcaacgaga acggtgatga ctctctggtt gtcccctaga gcttcgtaga
     2041 cataggaaag tctataaaga cttttagctg aaatctcggg ctggtttttc cctgaaatgg
     2101 tagagatggc tttgtaagta tctgcggcct cggtccagcg acccagctcc tcgagagctc
     2161 ccgctttcat aaatcgcgct cgctgaagct tcaaagaata aggataggcc gtttggaagg
     2221 tctcaatact ttgcaacgcc gcctcgaaat tttgttcttt cagcttctgt tcaatgagtt
     2281 gaaagtcgtc attttcgcga gcctccaaaa taaaggcctg agttggttgc tgctttttat
     2341 gaaaaacggg aaataaagaa caaccagcga aaaggaaaat caccgataag attatccata
     2401 atctcacttt tagccctctt tttctttcaa aatctctgcc actttgatca agccatgaag
     2461 atctgagata ggatccgaaa gctccggcag tcttaatact tgaacactct tactaatatg
     2521 agccgcaaat tttttatgct cacgatcccg ctgggagtaa aaagctttct cctgttgata
     2581 taaagtgaaa atcttttcgc tgccggcagg agcttcgtac aaaggatttt ctttatcgga
     2641 aaaccactca ggataggaac gattcagaat cacagagcgc agattatagc cgcctttacg
     2701 aatttcgcgc agaaagtatt ctgcctctaa aagctttgcc tgatcgaaac tggtcaccag
     2761 acaaaattgc gtatcagaac tgaccatcat gcggtgaaca ttgataatcc gggcttcgag
     2821 gtgctcttgc cactgttcaa tgctcatgaa gaaatccgaa agctctctca gaagttcgct
     2881 gccagtcacg gactccatga tattcaaaac ttgcttggtt ccggtgttca gaatattcag
     2941 aaaagttccg cccaccgatt tatttttcga ctcacgaaac caacgggcca ctttgtcgtt
     3001 aaaaagcacg gccagttttt ggggcgcgtt taaaaaatct atggcatgtt ttgacggagg
     3061 cgtatctaaa ataatcagat caaattcttt ggattcaaaa caagaataaa gtttttccag
     3121 cgctgtgaaa tcctgtgacc cactcagact agttgaaagt tgttgataga gtctgttttg
     3181 aaacaacttt tttgccgact ctgatttccc tgccgctctt aatacgaaat catcaaaggt
     3241 ttgcttatga tcaacaatgg aggcaaagag ttccccttta aagttctggc cgggaacgcg
     3301 ggtaatgtct ttcttacctt caatacccaa agtcgtcgcc aagcgttttg ccggatcaat
     3361 ggtcaaaact aaaaccttga gaccctgttg tgctgcccgg actcccatgg ccgcggccaa
     3421 ggtggttttt ccgacgccac cattgcctac acaaacgagt attttcatga gagcctcgaa
     3481 ctgatctggg ctaatacttt ctccacctca tggtcccaaa tcgtaggtaa gcattgcacc
     3541 tgggtatcca ttctttgcaa acgtattttc atctcgtgat gataatggcg atttctttgg
     3601 tcatcttcag caaaagctcg aaattcttca aaagaagaag tttgcgctga gacatactcc
     3661 ttcaagggaa ttgcctcctc taagaattta ttaagaacaa gttttgcttg aataccgtaa
     3721 tgatctttca gttgctgata gagttcttcg ctttcccgca cgggaagctc ttcgggaagg
     3781 caaactaagt agtattggca aaggcttggg tctcgcaaaa ctttgtcgat accacggctt
     3841 tgctctccca tggggccaaa ctttactgcc atcgccattc cttctgcggc tcccataaga
     3901 gccatgaaat ggcctgtggc ataacagtcc acgacgaggc agtcataaga aagaggaggg
     3961 ccatgatgac ggggtccact agtggctttt cccataatgg caagttctgg taacgcaggc
     4021 gccacattaa taaaggcctt catcacggca ttttcaaaaa aaagccggta cagagtttca
     4081 actttaatga gatggataat atattctcga aggcaatttt gccctgtcca cagggctact
     4141 gaaaaatttt ccttcacttt tactggctca aatcccacct tcgggagctg aaaataatct
     4201 tggtaaaagc tctggtcccc tatttccaca agcaaagttc gctgaccggc ttgagccttt
     4261 ttcagagcca aagctgctgc tacggtggac tttcccaccc ctcccttgcc ggtcacgaat
     4321 ataatttctg atttcatagg tcctcacagt gtcgctgagc ttggaatcta aggcattacg
     4381 ctcggcggca attgcttgct attcagaggg caatcttcta gtattccgat tcattttgac
     4441 gctactgcaa aactgaggag ccctcatgaa aaccaatatg gagaaaattt cttctctgca
     4501 tcacaaaatc aatgttcaag ttcctgtaac ggttgtgcaa acagctttca acagaatctt
     4561 taacagcatt caaaaagaag taagtataaa aggattccgt aaaggtaaag cgccactcac
     4621 caccattagg agtatgtaca acgaccgcat aaaacaggat gtggctcaag agttgattca
     4681 aatgcactac atcaaggctt tgactgaaca taagctcgag cctattagca accctgaatt
     4741 tgaattcgac attcctgctg aaacgaaaga attttctttc tccgccactt tcgatgttcg
     4801 ccccgaagta aacttgaaaa agtatgaagg tttcgaagtc gaaaaagaaa agtttacttt
     4861 cgacgataaa aaaatcgacg aagttcttca gaacatccgt aattcacgtg cgtctttcga
     4921 agatgtactt gaagttcgcc ctgctcagat gggcgatgtc gcgattattg atttcgctgg
     4981 tttcgtagat ggaaagcctc tcgaaaacgg cacaggtact gatcataacc ttgacctggg
     5041 caccaaacaa tttatcgagg gttttgaaga aggcatcgtc ggtatgaacg tcggcgattc
     5101 aaagacttta aacttgaaat tcccaaatcc ttaccactct gtagagttgg caggaaaagc
     5161 agtcgagttt aaggtcacac ttaaagggat gaaaaagaaa gtattgcctg agctgaacga
//
LOCUS       DATBTE010000415         1911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2381200, whole genome shotgun sequence.
ACCESSION   DATBTE010000415 DATBTE010000000
VERSION     DATBTE010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2381200"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VIG33_15965"
     CDS             complement(<1..785)
                     /locus_tag="VIG33_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272388.1"
                     /translation="MKPFKFCLKNSFVFTALISGCATTSPPPVSISKSNLGSDKIFGQ
                     YCANNKQIRRLAVSIEYITPKLPKMSVDKAAGESAYYAKRKQDLLKKTLFSLADGEEH
                     PNGRGADYLKVRQSDLNHHDLVEQISQYVIEENSYNSTSVKELLMRPTDTDKFILFLA
                     IETDQRLRDRDHYEPTYISELKLLVDNFTLLEPLEFQNEAYKCDQDCLKNPDMDHDKI
                     DLIAVDDEGKQSISDAVKEKDAIWIRYIKHFAKIKEAKSSNPRV"
     gene            complement(754..1446)
                     /locus_tag="VIG33_15970"
     CDS             complement(754..1446)
                     /locus_tag="VIG33_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272389.1"
                     /translation="MKTLIMASIFIASAAHAKIPTWAANNSTKLNGSSLRTVCHGVGP
                     SVDIARSEALKSCQLSASQFFISKIKVKSLSVETEKSVGYHQEVSSDDELSNLICDPT
                     KDELEETDSQYSIWLECKFDLNRVKAKPIKDSSDPVTAEDTNLSYSKPSAMTSDHSSK
                     TVFLSTIPKCESIIIKGDRPRTVECKSNPVELDISDADKKAIIRVREYKPKTIQLKKR
                     TDNETIQVLFEK"
     gene            complement(1461..>1911)
                     /locus_tag="VIG33_15975"
     CDS             complement(1461..>1911)
                     /locus_tag="VIG33_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272390.1"
                     /translation="VSTGMTTIPASDRVEAAYRIAQNNAKAAIAGAIEQKLEFIFQNA
                     EEGTSINATQARYIGAEASNLVTSTIRPGKNYWEKVATTQDSGERLTQYKVFSTVTMP
                     EDEFKRAIFEAIKKSQGKGGLSKDFAKKVDAHWDKFVENNQPAQESK"
BASE COUNT          471 a          379 c          396 g          665 t
ORIGIN      
        1 actctcgggt tgctcgattt agcttctttg attttggcaa aatgtttgat gtaccgaatc
       61 caaattgcat ctttttcttt cacggcatct gaaattgatt gtttgccttc gtcatcaaca
      121 gcaataagat caattttatc atgatccatg tcaggatttt ttaaacagtc ctgatcacat
      181 ttgtaggctt cattttgaaa ttcaagaggt tctagtaggg taaagttatc caccagtaat
      241 tttagctctg aaatataggt tggctcatag tgatctctat ctcgaaggcg ctggtcagtt
      301 tcgatcgcaa ggaataaaat aaatttatcg gtgtcagttg gcctcatcag cagttctttt
      361 acactcgtgg aattataaga attctcttcg atcacatatt gggagatttg ctcgacaaga
      421 tcatgatgat tgagatcgga ttgtctcact tttaggtagt cagcgcctct gccgttgggg
      481 tgttcttctc cgtcagccaa gctgaacaat gtttttttga gaagatcttg ttttcttttg
      541 gcataataag ccgattcacc agctgcttta tccacagaca tttttggtaa ttttggtgtt
      601 atgtattcga tggaaacagc tagccttcga atttgcttat tgtttgcaca atattggcca
      661 aaaattttat cacttccaag attactcttt gatatcgaca ccggaggagg gctggtcgta
      721 gcacatccgc taataagagc tgtaaagaca aagctatttt tcaaacaaaa cttgaatggt
      781 ttcattatct gtcctttttt tgagctgaat tgtctttggc ttatattctc gcaccctaat
      841 aatggccttt ttgtcggcat cagatatatc caactcaacc ggattacttt tacattctac
      901 ggttcgaggc ctgtcacctt taataataat tgattcacat tttgggatgg tcgacaaaaa
      961 tacagttttg cttgaatggt ctgacgtcat tgcagagggc ttgctatatg aaagattggt
     1021 gtcctctgct gtgacggggt cagaagaatc ttttatgggc ttagctttaa cccgatttag
     1081 atcaaatttg cattcgagcc aaatactgta ttgtgagtct gtttcttcga gctcatcttt
     1141 ggttggatcg cagatcaaat tactcaactc atcgtcgctt gaaacctctt gatgatagcc
     1201 aacagatttt tcagtctcga ctgagagcga tttaactttt attttggata tgaaaaactg
     1261 gctggcactt agctggcacg attttagcgc ttcactgcgc gcaatatcaa cagatggtcc
     1321 aaccccatga cagacggtgc gaagcgagga cccatttaat ttagtgctgt tattggctgc
     1381 ccacgttgga atcttggcat gggccgccga cgctataaaa atggaggcca taataagtgt
     1441 tttcatatgg cccatattga ttatttgctt tcttgagcgg gttggttgtt ttcaacaaac
     1501 ttatcccaat gtgcatcaac tttttttgcg aaatcttttg ataacccacc tttgccttga
     1561 gattttttga tagcttcaaa aattgctcgc ttaaattcat cttctggcat ggtgacagtt
     1621 gaaaatactt tatactgagt gaggcgctca cctgaatctt gggtcgtggc gactttttcc
     1681 caatagtttt ttccaggtct aatggtgctg gtgactaggt ttgatgcctc tgctccaata
     1741 tatcgagctt gtgttgcatt tatcgaggta ccctcttccg cattttgaaa tataaactct
     1801 agtttctgtt caatagcacc tgcaatggca gcttttgcat tgttttgtgc tattcgatag
     1861 gctgcttcga ctcgatcgct tgcagggatg gttgtcattc cagttgaaac g
//
LOCUS       DATBTE010000416         8008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1560205, whole genome shotgun sequence.
ACCESSION   DATBTE010000416 DATBTE010000000
VERSION     DATBTE010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560205"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VIG33_15980"
     CDS             <1..452
                     /locus_tag="VIG33_15980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272391.1"
                     /translation="ISVVDFPQGIHCTLPVPYDWKAISTILQSYFLSHSPNFKLKEQA
                     LEMELGDESNRDFYNGAIKVSYKVQQPEPKENFIRLTVELENEKEKRETLKLNLPLSQ
                     FDFHFFNKELSSSEQAMMERWVNQNLSMKVITKEGKPALRATWYLAP"
     gene            complement(491..979)
                     /locus_tag="VIG33_15985"
     CDS             complement(491..979)
                     /locus_tag="VIG33_15985"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY8272392.1"
                     /translation="MSDENIYSFQVKTPTNENMSLEKYKDQVLLIVNVASKCGFTPQY
                     TGLETLYEKYKNQGFTVLGFPCNQFGAQEPGTAEEIKDFCKLNYGVSFPLMAKIDVNG
                     NSADPLYTWLKQAAPGIMGTEAIKWNFTKFLIGKDGQVIKRYSPNTEPKDIATDIEQA
                     LK"
     gene            complement(1168..3000)
                     /locus_tag="VIG33_15990"
     CDS             complement(1168..3000)
                     /locus_tag="VIG33_15990"
                     /inference="COORDINATES: protein motif:HMM:NF025563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HEY8272393.1"
                     /translation="MRKSISFLRVNASFAVLMAMALNSGCTKSGFSLENRSSQDQTSM
                     GTLSKPRYQSMVTISPTSASLETNSSRTFQASGGSGGYIFSASAGRIASEGNIASFTS
                     PSFAGTVTITVQDSRGHIAQSRVSVTAPVSVPSTQPTSQATSIATGTYRIANACNQSL
                     IAAISPGLALGEPIVLQTKASPVVSSQVYKIAPDTMGGYKFINSNSSRSFGISSGSLT
                     EGATLSEWDYVNVPHQSFTFFKGADNFYRIKVKHTQMYLETAGISAGSGIVQKNLVDT
                     CNQKFALELVSVPVTAPAPAPAPAPVSTPTPTPQPPPPTGANILFKSGFENGVNLGTP
                     YSCGSTECSQQLTGQDAGGTSWPVSFWGSSNNSYLQLLSTVPVTPSTIGNYVKNEIQT
                     VTGRNGTPTRAFYQTLLGGYGGNSVRQDPYIIGPTNSVAQQGNMYIRYWVKFQADMAT
                     NQLIPGQTPDGGYGMWRVLSEWKSGGQGSNWQGDYRFIIGINMNSSRQLNWYATADNV
                     ANSGAPKTVYYHKADSGVPVPTGTWFKFETYTHRSSGSDGRLWAAVNGRVIADFRGPN
                     MGVNNLPINRIMLFQNYTGGYYPCYQWIDDVEVFDGFPSDATSH"
     gene            3218..4771
                     /locus_tag="VIG33_15995"
     CDS             3218..4771
                     /locus_tag="VIG33_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272394.1"
                     /translation="MKYFFTIFFIGFILFGPFAATAGSTADVEVTDGYLLSDPGSWKF
                     VNQPTQPQRPKPNTRNSTSGKQRTRSSPNAKSKPTNNNDPSMTENFPTAADGTKNCYK
                     NETCPPEQWNNISCTCVDPSNANNPNTTEGQNRTARSAAMNAAIQKCSQDTDVAKSDC
                     DQDHDPGIQGAQTTLSNFAVETGSQLGIAGACSGMAKYVAGANAAVIYFTQNCSSSRS
                     NCVTSCQKARTAVNGASAATQSEVTDLLNSCKDLDTKIQQGTQAIQNLVGTIQSSQSC
                     AKQTDAALVDYCASNPGAIGCSALSTDCSNPSVAASNQICICKNNPGSSACTGALAKT
                     SGGGFDSANMNALPGGAGGAAGVGSPGSDNLMGDANWAGDPNLKADKRASEEAGGKKG
                     GRPLLDGGGGGGAGGAGDKGKGGGTPAQGIAVNAGFRGGGGGGGWGGEGNGGGEGGYA
                     AQLPSEAGAKGPNLRDFLPGGGLDPKTVSRGLAGISGPDGITGPHSDIWKKVQHRYQI
                     QVEKSTLMP"
     gene            complement(4788..6008)
                     /locus_tag="VIG33_16000"
     CDS             complement(4788..6008)
                     /locus_tag="VIG33_16000"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY8272395.1"
                     /translation="MSTPKVIIQKYGGATLADPLKIKAVAKRVAEQARSATRLVVVVS
                     AMGKTTNSLIELSTQISPNPHQREMDMLLSVGERISMALMSMALNDLGCPAISFTGSQ
                     AGILTTDSHVNAFIVDVKGFRVQEALDQGKVVILAGFQGVSPVTKEITTLGRGGSDTS
                     AVAMAAFFQASRCEILKDVPAVFTADPNLVKEARPLQELNYDQLLEMTFWGAKVLHYR
                     SVELAKLRKVSLYIGPAATKTSEGTLVEEGLNMFESCKALSLNSHETVLQIGCARDSA
                     STALESLKKYFENKQIAFPQLLDLESTDHRTLFLMTGPQEIIKAIKNALAHHEDFYLN
                     PTDFSSVTLTCTGSTTPEIPQEVMKVLEQAKIRTYKVMIHAMSVTVLLEAAARAAALQ
                     VLHKLIEPRNLLNS"
     gene            complement(6018..6956)
                     /locus_tag="VIG33_16005"
     CDS             complement(6018..6956)
                     /locus_tag="VIG33_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY8272396.1"
                     /translation="MKWYFLILLGSFSLGSLAAQKPSTESITPSLKEKLQSLTLKNSH
                     TVVNNNTLIFDLPVTYNKKVSKWVHYYQSRGQKWFREWLARSSKFMPFIQTELQRANL
                     PMDLAFMVMIESGFSASAVSEAEAVGPWQFIAPTGSRYGLQKNWWLDERRDLKKSTLA
                     AIRYLRELHEEFGSWYLVAASYNMGEAGLRRQIKKYQTKDFWTLVQKGALPEETQEYV
                     PKILAAMLIAKAPNLYGFRDIEKLDPLNYDIVSVPGGTDLDGVADHLGITRKSLKDLN
                     AEILLGYIPRQIDRHFIRVPKGASAMVSAYTGKSRE"
     gene            7373..>8008
                     /locus_tag="VIG33_16010"
     CDS             7373..>8008
                     /locus_tag="VIG33_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook basal-body protein"
                     /protein_id="HEY8272397.1"
                     /translation="MSTKGVYTALSGALAQSLKLDTIANNLANVNTPAFKRDQQLFNE
                     YLTANEKPPEVIQVPRVPASIESFYDMQGGDKSFVDTKGTFTDFSQGGLKPTGNTLDV
                     AVDGKGFFEVATPSGVKLTRAGNFTMDGNGQLVTKDGFPVLKNGELGSDPAARTIRFE
                     GSGPVSINDSGDVYQGTELLGRLSLVEVKNPDSLQKMGNSLYGFKKNMAPEM"
BASE COUNT         2216 a         1721 c         1914 g         2157 t
ORIGIN      
        1 atatcagtgt ggtggatttt cctcagggga ttcactgtac attgccagtt ccctatgatt
       61 ggaaagcgat cagtaccatt ttgcagtctt atttcttgtc ccattcccca aattttaagc
      121 tcaaagaaca ggcccttgaa atggagcttg gagatgagag caatcgagat ttttataatg
      181 gcgccattaa agtcagttat aaagttcaac agcctgagcc aaaagagaat tttattcgtc
      241 tcacggtgga gttggaaaat gaaaaagaaa agcgagaaac tctaaaatta aatctccctt
      301 tatctcaatt tgattttcat ttttttaata aagaattgag ttcgtccgag caagctatga
      361 tggaacgatg ggtgaatcaa aacctttcga tgaaagtgat caccaaagaa ggaaaacccg
      421 ccttaagggc tacttggtat ttagcccctt agagtttata tttcgatctt gagttcaatc
      481 tcaatcttcc ttacttcaaa gcctgttcga tatctgtggc gatgtctttt ggttctgtat
      541 tcggggaata tctttttatg acttggccgt ctttgccaat taaaaattta gtaaaattcc
      601 atttaatagc ttctgttccc ataattcccg gagcagcttg tttaagccag gtatacaatg
      661 gatcagcact gttcccgttc acatctattt tggccattaa aggaaaactc acgccatagt
      721 tcagtttaca aaagtctttg atttcttcag cggtccctgg ctcttgagcg ccaaattgat
      781 tacacggaaa tccaagaaca gtgaaacctt gatttttata tttttcataa agcgtctcta
      841 gaccagtgta ttgtggagtg aacccacatt tgctggcgac gtttactatc aaaagcactt
      901 ggtctttgta tttttcaagt gacatatttt catttgttgg tgtttttacc tggaaggaat
      961 aaatattttc atctgacatg gtatgactcc tttgataaag ttctattata gataatagat
     1021 gcagctaggg ctctgtaaac atagcaacat ataagaaggc ttgaaaactt taaaatttag
     1081 aagtcactgc gcgatagctt gcgataaaaa aattgccggg gtgaatgccc cggcaaatgt
     1141 gattaagcga tgttaaaaag gtgaacttta gtgactagtg gcatctgatg ggaagccatc
     1201 aaaaacttca acatcatcga tccattgata acatggatag tagccgcccg tgtaattttg
     1261 gaagagcatg atacgattga taggtaagtt attcactccc atattggggc ctctgaaatc
     1321 agcgataaca cgaccattta ccgcagccca aagtcttccg tcactgccgc tacttctatg
     1381 ggtataggtt tcgaatttga accaagttcc tgttggaaca ggcacacccg aatctgcttt
     1441 atggtagtaa acagtttttg gagctccgga gtttgccacg ttgtccgcag tagcgtacca
     1501 attaagctgg cggcttgagt tcatgtttat tccaataatg aagcgatagt ctccttgcca
     1561 gttggatcct tgtcctccac tcttccactc ggaaagaacg cgccacattc catatcctcc
     1621 gtcaggagtt tgaccaggaa tgagttggtt cgtcgccata tccgcctgaa acttaaccca
     1681 ataacgaatg tacatatttc cttgttgagc aacactgttc gtagggccaa tgatgtaagg
     1741 atcttgacgg accgaatttc caccataacc tcctaatagt gtttgataga aggcacgggt
     1801 aggagtcccg ttacggccag tgacagtctg aatttcattt ttaacataat ttccaattgt
     1861 tgagggcgtt acaggtacag tgctgagaag ttgcaaataa ctattattgc tgctgcccca
     1921 gaagcttacc ggccacgaag ttccgccagc atcttggcct gtgagttgct gtgagcattc
     1981 agtgctgccg caagagtagg gtgtgcctaa gtttacgcca ttttcaaatc cagatttaaa
     2041 tagaatattc gcgcctgttg gaggcggtgg ttgaggtgtt ggcgttgggg ttgatactgg
     2101 agctggagca ggagcaggag caggagcagt cactgggaca ctcactaatt ccagagcgaa
     2161 tttttggttg catgtatcaa cgaggttctt ttgcacgatg ccggatcctg cactgattcc
     2221 tgcagtttct aaatacatct gagtgtgttt aactttaatt cggtagaaat tatcggcacc
     2281 tttgaaaaag gtaaaacttt gatgtgggac attcacatag tcccattctg aaagggtggc
     2341 tccttcggta agagaaccac tggagatgcc gaacgagcgt gaactattgc tattaatgaa
     2401 tttataacca cccatagtat caggtgcaat tttgtaaacc tggcttgaaa ccacagggct
     2461 ggcttttgtt tgaagtacga taggctcccc aagagctaaa ccggggctta ttgccgcgat
     2521 caagctttga ttgcaagcat tagcaatgcg ataagttcca gtcgcgatag aagtggcttg
     2581 ggatgtaggt tgagtggagg ggactgaaac aggcgcggtg acgcttactc gagattgggc
     2641 aatgtgtccg cgagagtctt ggacggtgat agttaccgtg ccggcgaagc tcggagatgt
     2701 aaagctagcg atattaccct ccgaagcgat ccttccagcg gaagcggaaa atatatatcc
     2761 accacttccc cctgaagcct ggaaggttcg tgaggaattg gtctcaaggg aagcgctagt
     2821 gggactaatg gtgaccattg attggtatct aggcttggaa agagtcccca tacttgtttg
     2881 atcttgagaa gaacggttct caagagagaa tcccgattta gtacaaccag aattcaatgc
     2941 cattgccata agaacggcaa aagatgcatt cactcgtaaa aacgaaatag attttctcat
     3001 aatgattacc cccacctgag tttgttgcaa gagtggggcc acagaaaaag tactccaaac
     3061 tcattgagga gcttcaaatt ttcgataaat tggcagttta ttaacaattg taaaaagata
     3121 gtcaacacta gcaatattct agtcatttta tgtctcaacg tggaattgct ctttaatatt
     3181 tccccaggtg agccgatact taagtagggg aagattcatg aaatactttt tcacaatatt
     3241 tttcataggg ttcattttgt ttgggccttt tgctgcgaca gccgggtcca ctgctgacgt
     3301 agaggtcact gatggatatt tactttctga tccgggaagc tggaagtttg taaatcagcc
     3361 cacccagcca cagagaccga aacctaatac cagaaacagc actagtggaa agcagcggac
     3421 gaggagtagc cctaatgcga aatcgaagcc tactaataat aatgacccta gcatgactga
     3481 aaatttccct accgctgctg atggaactaa aaactgctac aaaaatgaaa catgtcctcc
     3541 tgagcaatgg aataatattt catgtacgtg cgtcgatcca tctaatgcga ataatccaaa
     3601 cactacagaa gggcagaata gaacggccag gtcagcggcc atgaatgccg cgattcaaaa
     3661 atgctcccaa gatacggatg tcgctaaatc tgattgtgat caggatcatg atcccggcat
     3721 tcagggtgcc caaaccactc tttcaaattt tgcggtggag accggatcgc aattaggaat
     3781 tgctggagct tgtagcggaa tggcaaaata tgttgcgggt gcgaatgccg ctgttattta
     3841 ctttactcag aattgttcaa gttcaagaag caattgtgtt acgagctgtc aaaaggctcg
     3901 aacggctgtc aatggagcct cggcggcaac gcaatcggaa gtcacggatt tattaaattc
     3961 ctgtaaagat ctcgatacca aaattcagca aggcactcaa gctattcaaa acttggttgg
     4021 gactattcaa agttcacagt cctgtgcgaa gcagacagat gcggctcttg tggattactg
     4081 tgcttcaaat cctggggcca ttggttgctc agcattgtct acggattgct ctaatcccag
     4141 cgtagctgcc agtaatcaga tttgcatttg caaaaataat cccggcagtt cagcttgcac
     4201 tggggctctt gctaaaacca gcggtggggg ttttgactct gcgaacatga atgctcttcc
     4261 cggtggagcc gggggcgctg cgggggttgg ttcgccaggt tcggataact tgatgggtga
     4321 tgccaactgg gctggcgatc ccaatttgaa agctgataag cgtgccagcg aagaagccgg
     4381 tggaaaaaaa ggaggccgac ccttattaga tggaggcgga ggcggtggcg ccggcggagc
     4441 cggagacaaa ggaaaaggcg gcggtacgcc tgctcaaggt attgcggtca atgcggggtt
     4501 ccgcggaggc ggtggtggcg gcggttgggg tggagaagga aatggtggtg gtgagggtgg
     4561 ttatgcggct caactgccat ctgaagccgg agctaaagga cccaacttgc gtgatttctt
     4621 gcccggtggc ggattagatc ctaaaactgt cagccgtggg ctcgcgggaa tctcggggcc
     4681 tgatggcatc acgggacctc actcagatat ttggaagaaa gtccaacatc gttatcaaat
     4741 acaagtggaa aaatcgactt tgatgcctta ggttcatcga agtgaggtta cgagttcaat
     4801 aaatttctcg gctcaatcaa cttatgcaat acctgcaaag ctgcagccct ggcggcagct
     4861 tctaaaagaa ccgtcacact cattgcgtga atcatcactt tgtaagtccg aatcttagct
     4921 tgctccaaga ccttcatgac ttcttgagga atctctggcg ttgtggaacc cgtgcaagtc
     4981 aaagtgaccg acgaaaagtc cgtaggattc aaatagaaat cttcatggtg ggcgagagca
     5041 ttcttaatag cctttataat ttcttggggg cctgtcatca aaaataaagt cctgtgatcc
     5101 gtactttcaa gatctagaag ttgggggaaa gcaatttgtt tattttcgaa atattttttt
     5161 agagattcca aggccgtact ggcggagtcc cgtgcgcaac caatttgtaa aaccgtttca
     5221 tgggaattta atgacaaagc tttgcatgat tcaaacatat tcagtccttc ttcgacgaga
     5281 gtgccttcgg atgttttcgt ggcagctgga ccgatatata agctgacctt acgcagcttt
     5341 gccagctcca cagaacggta atggagtact tttgcgcccc agaaagtcat ttctaacaat
     5401 tggtcataat ttaattcctg cagagggcgc gcctctttca ccagatttgg atccgcagtg
     5461 aagacggcgg ggacatcttt taaaatttca cagcgagagg cctgaaagaa agcggccatt
     5521 gccacggccg aggtgtcgga ccccccccgc cccaaggtgg tgatttcttt agtgactgga
     5581 gaaacccctt ggaaacctgc cagaatgacc actttacctt gatctaaggc ttcttgaacg
     5641 cggaagcctt tgacgtccac gataaaggca ttcacatgag aatcagtggt taaaattccc
     5701 gcttgactgc cagtaaaact aatcgccgga catcccaggt catttaaggc catgctcatc
     5761 aaagccatgc tgattcgttc accgacactc aagagcatat ccatttctcg ttgatgagga
     5821 ttaggagaga tttgcgtgga aagctcgatc agagagttcg tggtttttcc catggcgcta
     5881 acgacgacca ccaaacgggt cgctgagcgc gcttgttcag ccaccctttt tgcaacggct
     5941 ttaattttta ggggatccgc aagagtggcg ccgccgtatt tttgaataat aacttttggt
     6001 gtcgacatgt tgagggtcta ctcccgggat tttccagtat acgcggacac catcgcactc
     6061 gcgcccttgg ggacacgaat aaagtgtctg tcaatctggc gaggaatata tcctaaaagg
     6121 atctcagcat tcaaatcttt taacgatttg cgagtgattc ctaaatgatc cgcaacgcca
     6181 tcgagatccg ttcctccagg aacggatacg atatcgtaat ttaaagggtc caacttctcg
     6241 atatccctga atccataaag attgggtgct ttggcaatta acatggccgc caatattttt
     6301 ggaacatact cctgagtttc ctcaggaaga gctccttttt ggacgagcgt ccaaaagtcc
     6361 ttggtttgat atttcttaat ctgacggcgg agaccagcct cgcccatatt gtagcttgcg
     6421 gcgaccaagt accaagaacc aaattcttca tggagttctc ttaagtaacg aatcgcagcc
     6481 aaggtgctct ttttaaggtc ccggcgttca tccagccacc aatttttttg cagaccgtat
     6541 cgacttcccg tcggagcgat aaactgccac ggcccaacgg cctcggcctc actcaccgct
     6601 gaagcggaga aaccactttc gatcatcacc atgaaagcaa ggtccatcgg tagattggcg
     6661 cgttgcaatt cggtttgaat aaagggcatg aatttcgaag agcgcgccaa ccattcgcga
     6721 aaccactttt gacctcgact ttggtagtag tgcacccact tgctgacctt cttattgtaa
     6781 gtcacgggta aatcaaaaat cagggtattg ttattcacaa cggtatgact atttttcaac
     6841 gtgagactct gcagtttttc cttgaggctg ggggtaatac tttcagtcga aggcttttgg
     6901 gccgccagag agcctaaaga aaatgagccg agtagaatta aaaaatacca tttcataaat
     6961 ttattatggg tttagttttg aaggagcgtc gaggacaatg aggggagggg gaacaaaaac
     7021 taggcttcat ggagaaggat ttcagcatta ggacctttga ctaaaaaatc aagaactggc
     7081 gagaaactaa atttatgaag ataaaataat tagggttggc ccgttacaaa ctttaatagc
     7141 aggatccctc agaggattct ttgacagcat accccactgg cgacaaattt ctgtcacctc
     7201 gaccttttgt agagaaaagg cgcaaaaaaa aatcacggct cagatcaaaa taacttgagt
     7261 ctaattcttt ataggcaact aaaactcgac catagtgcag gctcacgtct gcatgaagaa
     7321 aaaaatctta ttggcatacg ccttgctcca atcttttgca aggggagtat aaatgagtac
     7381 aaaaggtgtt tacacggctc tgagcggtgc ccttgcacaa agtttgaaac ttgacaccat
     7441 cgctaataat ttagcgaatg tgaatactcc ggctttcaag cgtgaccaac agctcttcaa
     7501 tgaatatctc acagccaacg aaaaacctcc ggaggtgatt caagtcccgc gcgtgcccgc
     7561 atcgattgaa agtttttatg atatgcaggg aggagataaa agctttgtcg acaccaaggg
     7621 gactttcacg gatttttctc aaggcggttt aaaacccaca ggtaatactt tagacgtggc
     7681 cgtggatgga aaaggatttt ttgaagtcgc gactccctca ggagttaaac tcacgcgggc
     7741 aggaaatttc accatggacg ggaacggtca gctcgtgact aaagatggat ttcccgtttt
     7801 aaaaaatggg gaattgggct cggatcccgc tgcacgtacc attcgctttg aaggcagtgg
     7861 ccccgtcagc attaatgaca gcggtgatgt ttaccaaggg accgaacttt tgggtcgatt
     7921 gtctttggtg gaagtgaaaa atccggattc attacagaaa atgggaaact cactttatgg
     7981 gttcaaaaaa aatatggctc cagagatg
//
LOCUS       DATBTE010000417         1957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_603665, whole genome shotgun sequence.
ACCESSION   DATBTE010000417 DATBTE010000000
VERSION     DATBTE010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603665"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VIG33_16015"
     CDS             <1..351
                     /locus_tag="VIG33_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272398.1"
                     /translation="LYLAGWDCALMPFALNDSTRFISPTKTPEYLAAGKPVVSTAIRD
                     VVNPYGKNRLVYIAETAEDFVDMAEMAIREAQNDQRLRKVNKFLENTSWDNTWNRMAE
                     LEFKADKMKPALVL"
     gene            348..>1957
                     /locus_tag="VIG33_16020"
     CDS             348..>1957
                     /locus_tag="VIG33_16020"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HEY8272399.1"
                     /translation="MKTNSHNLSKDSKEPLEVWAGLECTVNRVGDDFFNQCDKSGHYR
                     REGDLEHFARLNIKKIRYPCLWEVVCQKEEGKYNWSELDKKLLEFQRLGLQPIPGLLH
                     HGSGPSFTSLMDPLFPEKFANYARDFAARFPWITSYIPIDEMLTTARFSCLYGHWYPH
                     HQSDGSFARALVNQVKASVLAMKVIRLINPSALFIQVDDIGRAQSTERLQYQATFENH
                     RRWLSFDLICGRVNQGHPLYAYLIEAEIHPEELSWFQENPYLPDMIGLNHYLLSNRFL
                     DHRLELYAPEYHGGNSREAYADLGAVDLKTVPLIEPEEIFSEAWERYKIPLAITEVHG
                     RGHREDQLRWFYQIWQSAKNLREQGVDIRAVTAWSLLGNFDWHKLCTVSENFYEPGVF
                     DLRSLEEPLKPTALSRMISSLATDQDFTHPVLENPGWWKIDRPPNFLQINTRAAPLVI
                     TGGAGTLGQAFAKICERRGIHYVLLQRSELDVADITSVAEKLNHLRPWAIINTAGYAN
                     VDLAEINAAQCFRVNTEGSGILAEYCADS"
BASE COUNT          496 a          416 c          500 g          545 t
ORIGIN      
        1 ctttacttgg cgggatggga ttgtgctctg atgcctttcg cgctcaatga ttcgactcgt
       61 tttatcagtc cgacgaaaac acctgagtac ttagcggctg gcaaaccagt ggtttccacg
      121 gccattcgtg atgtagtgaa tccatacgga aaaaacaggt tagtctatat cgcagagacc
      181 gctgaagact tcgtggacat ggcggagatg gccatacgcg aagctcaaaa tgaccaaagg
      241 ctgcgaaagg tgaataagtt tttagaaaac acctcttggg ataacacatg gaatcgcatg
      301 gcggaactgg agtttaaagc ggataaaatg aagcctgcgt tggtgttgtg aaaacaaatt
      361 ctcacaattt atcaaaagac tccaaagagc ctttagaggt gtgggcgggg ttagagtgca
      421 cggtaaaccg tgttggcgac gattttttca atcagtgtga taaaagcggc cattaccgga
      481 gagaggggga tcttgaacat tttgcgcggc tgaatattaa aaaaattcgg tatccttgcc
      541 tttgggaagt ggtttgccaa aaagaggaag gtaaatacaa ttggagcgag cttgataaaa
      601 agttgcttga gtttcaacgc ctgggtttgc aacctattcc ggggcttttg catcacggaa
      661 gtggtccttc ttttacaagt ttgatggatc ctctttttcc agaaaaattc gcgaactatg
      721 ccagggattt tgccgctcgt tttccttgga tcacttccta cattcctatt gatgaaatgt
      781 taacgacggc tcgcttcagc tgtctatatg ggcattggta tccgcatcat caaagtgacg
      841 gttcatttgc tcgtgctctt gtgaaccaag tcaaggccag cgttcttgca atgaaagtta
      901 tacgcctgat caatcccagt gctctcttta ttcaggtcga tgatataggt cgcgctcaaa
      961 gtaccgagcg ccttcaatat caagcaacct ttgaaaacca tcggcgttgg ctgagctttg
     1021 atttgatttg cggtagagtg aaccaaggtc atccgcttta tgcttatctt attgaggcgg
     1081 aaattcaccc tgaggagctt tcttggttcc aggaaaatcc ctatctgcct gatatgatag
     1141 gtttgaatca ttatttgctt agtaatcgtt tcttggatca ccggctggag ctatatgcac
     1201 cagagtacca tggggggaat tctcgggagg cttatgcgga tctaggggct gtcgatctga
     1261 agacagttcc tttgattgaa cctgaagaaa tttttagcga agcctgggaa agatataaaa
     1321 tccctttagc gattaccgaa gttcacggtc gaggtcatcg tgaagatcaa ctgcgttggt
     1381 tttatcaaat ctggcagtca gccaaaaact tacgtgagca gggtgtggat attcgggcgg
     1441 ttaccgcctg gagcctgctc gggaattttg actggcataa gctctgcaca gtttcagaaa
     1501 acttttatga gccaggagtg tttgacctca gaagtctcga ggaacctttg aagcccacgg
     1561 cgctttcccg aatgatttcg tctttggcga cggatcaaga ttttacgcat ccggtgcttg
     1621 agaatcctgg ctggtggaaa atcgacaggc ctccgaattt cctccaaatt aatacgcggg
     1681 ccgcgccctt agtgatcacc gggggcgctg gaactttagg tcaggctttc gctaagatat
     1741 gtgaacgacg cggcattcat tatgttcttt tgcaaaggtc cgagttggat gttgcggata
     1801 taacatccgt cgccgaaaaa ctcaatcatc tgcgcccctg ggctattatc aataccgccg
     1861 gctatgcgaa tgtggactta gccgaaatca atgctgcaca atgtttccgg gtgaacactg
     1921 aaggctctgg tattttggct gaatattgcg ccgattc
//
LOCUS       DATBTE010000418         1653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1424585, whole genome shotgun sequence.
ACCESSION   DATBTE010000418 DATBTE010000000
VERSION     DATBTE010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424585"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VIG33_16025"
     CDS             complement(<1..617)
                     /locus_tag="VIG33_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272400.1"
                     /translation="MKNLLHTLDNLGAPVSKLVKQPICDDKGNTLAWNFFVQNSKGIS
                     VAGGTHVDPAIAKRISIAEALERALAGQISRVDHLTKEFLLDKYPTSCGFACGFEKAP
                     TKMRALREATERWAWSMWIDKSFTINRLDKKNIIIDPLSKFIFEHFSNVQLFQKDDIL
                     IPFENEIVKYSFLVLILETDLGVFAGCRAGMPGENLLSHAAIEAFR"
     gene            complement(614..>1653)
                     /locus_tag="VIG33_16030"
     CDS             complement(614..>1653)
                     /locus_tag="VIG33_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272401.1"
                     /translation="LIFHLTVKPENFIGHLRLPYFGFWSPTNFNSSGEFKDDLKIISS
                     GPYTVSNSTKNLLILKKRPDWFSNSEASAKEIQFKYSTPGQLANFYGPTIVEILGRDQ
                     AIDLKSFHEIKAPPSLMWAMVLSPIKPGPFQNIRNRQAFVDRIVELKNKSSFFSSFFF
                     YPSKPTEIRMRDQKPDYSPEMRGQKIQFAFANRNYTQDEINEIKRMVIEALKDSLAEV
                     EFTVNDPLEDHWRQRLLSNQEFDIRMNGVDQGGYPKNRTIKMMFCSNLGVCYPDPSGR
                     ICKIVKQQDNAGGPISGNYISEFNQALYDDSTVIPLTHFGTRWLIADGIDLKTLPSNS
                     GIFYFEGIRLK"
BASE COUNT          498 a          308 c          312 g          535 t
ORIGIN      
        1 cgaaacgcct caatggctgc gtgggaaaga agattttcac caggcattcc agcccggcat
       61 ccagcaaaaa ctcccaaatc tgtctccagg attagtacta aaaacgagta ttttactatt
      121 tcattttcaa atggtattaa aatgtcatct ttttggaaca attggacatt actaaaatgc
      181 tcaaagatga atttagaaag aggatcaatg ataatatttt ttttatctaa ccgattgatt
      241 gtgaatgatt tatcgatcca cattgaccaa gcccatcttt ctgttgcctc tcggagagct
      301 ctcatttttg taggtgcctt ttcaaatcca catgcaaagc cacaagaagt tggatactta
      361 tcaagtagaa attcttttgt taaatgatcc acccttgaaa tttgtccagc taaggctcgt
      421 tccaaagctt cagcaattga gatccgtttg gcaattgcag ggtcgacatg ggtgccgcca
      481 gcaactgaga tgcctttgct attttgaaca aaaaaattcc aagcgagagt gtttcccttg
      541 tcatcgcata taggctgctt aacaagtttt gaaactggtg cccctaaatt gtccaaagta
      601 tgaagaagat ttttcatttt aatcgaattc cctcgaaata aaaaatcccg gaatttgatg
      661 gaagtgtttt taaatctatc ccatcggcaa ttaaccagcg agttccaaaa tgggtaagag
      721 gaattactgt tgagtcatca taaagcgctt gattgaactc agatatataa ttcccagaaa
      781 taggaccacc tgcattatcc tgttgtttta ctatcttaca aattctacca gatggatctg
      841 gataacagac tccaagatta gagcaaaaca tcattttaat ggttctgttt ttcggatagc
      901 ctccttgatc gactccattc attcttatgt cgaactcttg attactcagt aggcgttgtc
      961 tccaatgatc ttcaagtgga tcattaacag tgaattcgac ctccgcaaga gagtctttaa
     1021 gcgcttcaat aaccattctt ttaatttcat taatctcatc ttgagtgtaa tttctattag
     1081 cgaatgcgaa ttgtattttt tggcctcgca tttcaggtga atagtctggc ttttggtctc
     1141 gcattctaat ttcggtcggc tttgatggat aaaaaaagaa tgatgaaaaa aatgaagatt
     1201 tattttttag ttcgactatc cgatcaacaa aggcttgtcg gtttctaata ttttgaaaag
     1261 gacctggctt tattggagat aacaccatcg cccacattaa agatggcggt gccttaattt
     1321 catgaaatga cttaagatct atggcttggt ctctacctaa tatttcgacg attgttggtc
     1381 catagaagtt tgcaagttgg cctggcgttg agtatttaaa ttggatttct ttggctgacg
     1441 cctcagagtt ggaaaaccaa tcaggtcttt tttttaatat gagcaaattc tttgtggaat
     1501 ttgaaactgt ataaggacct gaagaaataa tttttaaatc atctttaaac tcgccggaag
     1561 aattgaagtt tgtcggactc caaaaaccga aataaggaag ccgaagatgg ccaatgaaat
     1621 tttctggttt gacggtcaaa tgaaaaatta att
//
LOCUS       DATBTE010000419         3543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_193981, whole genome shotgun sequence.
ACCESSION   DATBTE010000419 DATBTE010000000
VERSION     DATBTE010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193981"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2130)
                     /locus_tag="VIG33_16035"
     CDS             complement(<1..2130)
                     /locus_tag="VIG33_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY8272402.1"
                     /translation="MSSRLYWIFFSLLIVIEPSFAKKQEGKGLLPEVRIGENGSEAEN
                     DRKTLTSEILITRSENKAIDSLQKLIKGKKPGSPESADLLYRLAEMYMRRSKSGRFFD
                     MNANTPAVKLSPFPVPNERGADAVKRAIGVYTKIEKEHPRFKDMDSVLFNNAFAYQQI
                     GNHKEASAIFTRLVDKFPKSPLIADGTLALGELLYEQGKFPQALEQFLKLEKTPKSRV
                     YSYGMYKAAWSYYNMKDAENGTKRLLTVVKANPPLQEGEIPSNRHNLRKEALRDLTIF
                     VGDSYPADQLYPFFEKITQNNELGPAMIDLAKLYESHSRQKEMGIFLDEFIDKNPTNS
                     HLVKAHLYLIGGYEDLKQRDKVINHLQAASDLCKSNSSWKATQTPEEAGEYCDKDFRH
                     ESLNIAKKWWEIWLKNKQNVAFSDLTQKSFRLILDNEDTAKPDIKTHFALAELLFQLG
                     KYEEASREYKFVGDRAHEPTLQHDANYGALYAVEKQIEKSKDKEPARDALRKQLATNY
                     LTKHPTGKYALNVRFKMGHIAYEENNFPEAEKWLKQIADSNKDSELKKKSEDLMLDML
                     NMKKDYAGLQKLSKKIIATGVTEDRKKGMTKILEESSFAEVQEFSKTADPTQAARKLL
                     DFAREHENSPMAKDSLWQAISLLYANGRQFEAAELSLQYAKKYPQDKRTFDALKDAGK
                     SYAEVGQLLKAADTLKVVAEMDKKTSSA"
     gene            complement(2144..2422)
                     /locus_tag="VIG33_16040"
     CDS             complement(2144..2422)
                     /locus_tag="VIG33_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272403.1"
                     /translation="MKIDKTKIADMKNSNFEAKDIDPNDTNFILPLFNELDKLAKDEK
                     QKDNPAVKEGRILLQADSSLPYSVLRKVMYTASMAGFPQLKLVTLVGN"
     gene            complement(2432..2641)
                     /locus_tag="VIG33_16045"
     CDS             complement(2432..2641)
                     /locus_tag="VIG33_16045"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY8272404.1"
                     /translation="MGRRRYELPKQKSTFGLNITSMTDMFTILLVFLLQTYSTSNVEL
                     IPESGMRLPASSSNTNPVESIKVVV"
     gene            complement(2645..3193)
                     /locus_tag="VIG33_16050"
     CDS             complement(2645..3193)
                     /locus_tag="VIG33_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY8272405.1"
                     /translation="MRRTKKVKINHEYEFELDLAPLLAVMVKLVPVLLISSAFVQLMT
                     IETQLPQVVQQAIEKNDKDVKATHITLEVKEKEGVKIIVNKQGKESSEIVAKTSDGKF
                     DFVHLQERLREVKKQNPEVFKIDLSPDASISYDDIVKVMDECRRSRDANTKFPLFDKA
                     TNKETSTDYMFPEVTFANTMEG"
     gene            complement(3232..>3543)
                     /locus_tag="VIG33_16055"
     CDS             complement(3232..>3543)
                     /locus_tag="VIG33_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY8272406.1"
                     /translation="ILSFQAVAQADPAQKQAMLAHGISVSMYTTALGLAVAIPAMVFF
                     SFLTARQNQLIEQISEKCSKLTELLTSSHIPTLNRQTVFPDQVAAPKVQNVPPSNMKA
                     S"
BASE COUNT          863 a          728 c          812 g         1140 t
ORIGIN      
        1 tgcactgctg gtttttttat ccatctccgc gacgactttc aaagtgtccg ccgcctttaa
       61 aagctggccg acttccgcat aggattttcc ggcatctttt aaggcatcaa aagttctctt
      121 atcctgggga tatttttttg cgtattgcaa actcaattcc gccgcctcaa attgacggcc
      181 gttggcatag agcagactga tggcttgcca taatgaatcc ttcgccattg gagaattctc
      241 atgctctcgc gcgaaatcca ataatttgcg agcggcttgc gtgggatccg ctgtttttga
      301 aaattcctga acttcagcaa acgaggattc ttctaaaatc tttgtcatgc cttttttgcg
      361 atcttcagtg actcctgtgg cgatgatttt tttagaaagc ttttgtaacc ctgcgtagtc
      421 cttcttcatg ttgagcatgt cgagcatgag atcttcggac tttttcttga gttctgaatc
      481 tttgttagag tcggcgattt gttttaacca cttctcggcc tctgggaagt tgttttcttc
      541 ataggcgata tggcccattt taaagcgaac gttcagagca tattttccgg taggatgttt
      601 tgtcaggtaa ttggtcgcca attgtttacg gagagcatcc cgagcgggtt ccttgtcttt
      661 gctcttttca atttgttttt caaccgcata gagcgcacca taatttgcat catgttgcag
      721 agtgggttcg tgggcgcgat ctcccacaaa tttatattcg cggctggctt cttcatattt
      781 tcccaactgg aaaagaagtt cggccagggc aaagtgagtt ttgatgtcag gttttgccgt
      841 gtcttcattg tcgagaatta agcgaaacga tttctgagtt aagtcggaaa acgccacatt
      901 ctgtttgttc tttaaccaga tttcccacca ctttttagcg atattgaggg actcgtgacg
      961 gaaatcctta tcgcaatatt ctccagcttc ttctggtgtc tgcgtggctt tccaggaaga
     1021 gttggacttg cacagatcgc tggctgcttg aaggtgattg atgaccttgt cccgttgttt
     1081 aagatcctca tagcctccaa ttaaataaag atgggctttt accaagtggg agtttgtggg
     1141 gtttttatca ataaactcat ctaagaaaat acccatttct ttttgacgac tatgggattc
     1201 atagagtttt gccaggtcaa tcattgcagg gccaagttca ttattttgag tgattttttc
     1261 aaagaatggg tagagctggt ccgctggata actgtcgccc acgaaaatag ttaaatcgcg
     1321 cagagcctct ttgcgaagat tgtgacggtt gcttgggatt tccccttctt gcagcggcgg
     1381 attggctttt accaccgtta aaagacgctt ggtgccgttc tcggcatctt tcatattgta
     1441 gtaagaccaa gccgctttgt acattccata agaataaact cgagatttgg gcgttttttc
     1501 aagtttcaag aattgctcaa gggcttgggg aaatttacct tgttcgtaga gcaactcccc
     1561 cagcgctaaa gtgccatccg cgatcaaagg ggatttggga aatttgtcca caagacgggt
     1621 gaagatggct gaagcttctt tatggttgcc gatctgctga taagcaaagg cattattgaa
     1681 gagaactgaa tccatgtctt tgaaacgcgg gtgttctttt tcgatcttgg tataaacacc
     1741 aatagcgcgc tttacggcat cagcacctcg ttcatttgga actggaaagg ggctgagttt
     1801 gacggcagga gtattcgcat tcatgtcgaa gaagcggcct gattttgagc gtcgcatata
     1861 catttcagcc aagcgataaa ggaggtccgc actttcaggg ctgcctggtt tttttccttt
     1921 tataagtttt tgcagggaat ctatggcttt gttttcggag cgagtgataa gaatttcaga
     1981 agtcagggtt tttctgtcgt tttccgcttc gctgccgttt tcgccaatgc gcacttccgg
     2041 taaaaggcct ttgccctctt gctttttagc gaaagagggt tcaatgacta tcagaagtga
     2101 aaaaaaaatc caatatagac gtgagctcat aaaatttctc caactaattt cctacgagtg
     2161 tgactaactt caactgtgga aatccagcca ttgaagcggt gtacatgact tttcttaaga
     2221 ccgagtaagg aagggaagaa tccgcctgca ataaaatgcg cccttcttta accgcaggat
     2281 tatctttttg cttttcatct ttagcgagtt tatcgagttc gttgaataag ggtaaaataa
     2341 agtttgtatc gttaggatca atgtctttgg cttcaaagtt cgaatttttc atatcagcaa
     2401 tcttggtttt atcgattttc accaattctt tttaaaccac gaccttgatg gattctaccg
     2461 gattcgtatt ggaagacgag gcggggagcc tcatgccact ttcaggaata agctccacgt
     2521 ttgaagtgga ataggtttgt agcaagaaca ccagcaggat ggtaaacatg tctgtcattg
     2581 aagtgatatt caagccgaaa gtgctctttt gcttaggaag ctcatagcgt cttcttccca
     2641 taaattagcc ctccatcgta tttgcaaagg tgacttcagg aaacatgtag tcagtggatg
     2701 tttctttatt ggtggcttta tcgaaaaggg ggaatttcgt gttcgcatcg cggctgcgac
     2761 gacactcatc catgacttta acgatatcgt cgtaagagat gctggcgtcg ggagaaagat
     2821 cgattttaaa gacttcagga ttttgttttt ttacttcacg cagtctttct tgaaggtgga
     2881 cgaagtcaaa tttaccatct gaagtcttgg ctacgatttc gctgctttct ttgccttgtt
     2941 tattcacgat gattttcacg ccctcctttt ctttcacttc cagagtgatg tgagtcgctt
     3001 taacatcttt gtcatttttt tcgattgcct gttgcacaac ttgtggcaat tgagtttcaa
     3061 tcgtcattaa ctggacaaag gctgaagaaa tgaggagaac tggaaccagt tttaccatca
     3121 ctgccagcag cggtgccaaa tccagttcga attcatattc atgattaatt ttcacttttt
     3181 tagttcttct catataagat cccacccttt acttttaagg tctttttaag attaagaggc
     3241 tttcatattt gaaggaggaa cattctgcac tttaggagcg gccacttgat ctggaaatac
     3301 ggtttgacgg ttcagagtgg gaatatgtga gctggtcagg agttcagtca attttgaaca
     3361 tttctcagag atctgctcga tcagttgatt ctgacgggca gtcaggaatg agaaaaacac
     3421 cattgctgga atggccaccg caagacccaa agcggtcgta tacattgata cggaaatacc
     3481 atgcgccaac atggcttgtt tttgagcagg gtcggcttgt gcaaccgctt ggaaggataa
     3541 aat
//
LOCUS       DATBTE010000420         2794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_399489, whole genome shotgun sequence.
ACCESSION   DATBTE010000420 DATBTE010000000
VERSION     DATBTE010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399489"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /gene="rpsH"
                     /locus_tag="VIG33_16060"
     CDS             <1..270
                     /gene="rpsH"
                     /locus_tag="VIG33_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEY8272407.1"
                     /translation="GYIRSFKVAKDSKQGIMRVYLKYSEDGQHAITSLDKVSTPGRRL
                     YVKASQIPKVRSGFGMCILSTSKGILSDKQATEKNLGGELLCTVW"
     gene            278..820
                     /gene="rplF"
                     /locus_tag="VIG33_16065"
     CDS             278..820
                     /gene="rplF"
                     /locus_tag="VIG33_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEY8272408.1"
                     /translation="MSRIGKAPVIFDDKVQVSVTPKNEVFVKGAKSSLNISMLPAITA
                     SLDGKKVILSRADDSKESKALHGLYRSLIQNAVIGVTKGFTKILELHGVGYRANVAGK
                     KLELSLGFSHPVIFDIPEGIEIKVDKQVTISITGASKELVGQVAAKVRGFRPPEPYLG
                     KGVRYAGEHIRRKAGKSAGK"
     gene            836..1204
                     /gene="rplR"
                     /locus_tag="VIG33_16070"
     CDS             836..1204
                     /gene="rplR"
                     /locus_tag="VIG33_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEY8272409.1"
                     /translation="MKLKMSKHTSERKQNRLTKKIRIRKTVNGTEERPRLCVFRSGKH
                     MYAQVINDVNGHTLASASSVNIETENSGIDLAKEVGVAVAKSALAKNITNVVFDRNGY
                     LYHGRVKSLADGAREGGLNF"
     gene            1214..1669
                     /gene="rpsE"
                     /locus_tag="VIG33_16075"
     CDS             1214..1669
                     /gene="rpsE"
                     /locus_tag="VIG33_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEY8272410.1"
                     /translation="MEKISTAEFEERVVAINRVTKVVKGGRRFSFAALVVVGNGIGDI
                     GFGSGKAGEVPEAIGKASRAAKKNSKGINLKEGTIPHEVIGKFGAAKVVMKPAAPGTG
                     VIAGGAVRAVLESVGVKDILTKCVGTRNPHNAVRATIDGLKQLKQMKQI"
     gene            1697..1882
                     /gene="rpmD"
                     /locus_tag="VIG33_16080"
     CDS             1697..1882
                     /gene="rpmD"
                     /locus_tag="VIG33_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEY8272411.1"
                     /translation="MAKTFIVTLKRSLIGCTQDQKDTVRCLGLKKIRQSAEVNDTKAN
                     RGQIMKVQHLITVDVKG"
     gene            1886..2323
                     /gene="rplO"
                     /locus_tag="VIG33_16085"
     CDS             1886..2323
                     /gene="rplO"
                     /locus_tag="VIG33_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEY8272412.1"
                     /translation="MSLLSSLAPKVGSKHAPKRVGRGIGSGLGGTAAKGHKGQLARTG
                     GKVRRGFEGGQTPLMRRLPKFGFSNVAFATEYDIVNLGQLDKLSGEVTPEVLYQHGLI
                     GKGLVKVLGNGELKKALTVKAHKFSEGAKKAIEAAGGKVEVIK"
     gene            2323..>2794
                     /locus_tag="VIG33_16090"
     CDS             2323..>2794
                     /locus_tag="VIG33_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEY8272413.1"
                     /translation="MSAVDSIAKNSDLRNRIFFTLAMLAVYRIGVHVPTPGVDGGAVL
                     SFFNAQSSGIFGLFNTFTGGALSQFSIFALGIMPYISASIIFQLLTSAVPYLESLKKE
                     GQQGTKKINQYTRYATVVLAVIQGYGISSWLMNSTSPTGQSLVIAPFVGFLPFQV"
BASE COUNT          821 a          480 c          675 g          818 t
ORIGIN      
        1 ggttacattc gtagctttaa ggtggctaaa gattctaagc agggtatcat gcgcgtctat
       61 ttgaaatact cagaagatgg tcaacacgcg atcacttcac ttgataaggt aagtactccc
      121 ggtcgtcgct tgtacgtaaa agcctctcag attcctaaag taaggtctgg ttttggcatg
      181 tgcattttga gtactagtaa aggaatcctg agtgacaagc aagctactga gaaaaatctt
      241 ggtggtgaac ttctttgcac tgtttggtag gtgaaatatg tctcgtattg gtaaagcccc
      301 agtaattttt gatgataaag ttcaagtaag tgtgactcct aagaacgaag tctttgttaa
      361 aggagcgaaa agctctttaa atatcagcat gttgcctgcg attacggcaa gccttgatgg
      421 taagaaagtt attctttctc gtgccgatga ctctaaagag agcaaagcgc ttcacggctt
      481 atatagatca cttatacaaa atgcggtgat tggtgttact aagggtttca caaaaattct
      541 tgaacttcat ggtgttggtt atcgtgcaaa tgttgcagga aaaaaattgg agctttcttt
      601 gggcttcagt catccagtaa ttttcgatat acctgagggt attgaaatta aggtcgataa
      661 acaagtaact attagcataa caggtgctag taaagaactt gtgggacaag tggctgcaaa
      721 ggtccgtggc tttcgtccac cagagcctta ccttggaaaa ggtgtccggt atgctggaga
      781 gcatatcaga cgtaaagctg gtaaatctgc tggtaaataa ttgaggtgag ttatgatgaa
      841 attgaaaatg agtaaacata ctagtgaacg caaacaaaat cgcctcacta aaaagattag
      901 aattagaaaa acagttaatg gaactgaaga acgtcctcgt ttgtgtgtct tcagaagcgg
      961 caaacatatg tatgcgcagg taataaatga cgttaatggt catactcttg catccgcttc
     1021 ttcggtaaac attgagactg aaaattcagg tatagattta gcaaaagaag tgggcgtagc
     1081 agtcgcgaaa agtgcgttag ctaaaaatat cacaaacgtg gtttttgata gaaatggcta
     1141 cttgtatcat ggtcgagtta agtctcttgc agatggcgcg cgtgagggcg gcctcaattt
     1201 ctaaggagta attgtggaga aaatttcaac agctgaattt gaagagcgcg tagtagccat
     1261 caaccgtgtt acaaaagttg taaaaggcgg tcgtcgtttc tcttttgcag cattggtcgt
     1321 tgtaggtaac ggtattggag atattgggtt cggctccggt aaagcgggag aggtgcctga
     1381 ggcgatcggt aaagctagtc gtgcggctaa aaagaattct aaaggcatta acctaaaaga
     1441 gggcactatt cctcacgaag ttattggtaa atttggagct gctaaagtgg ttatgaagcc
     1501 tgcagctcct ggtaccggtg taattgctgg tggtgcggtt cgtgcggttc ttgagtctgt
     1561 tggggttaag gatattctta ctaaatgcgt gggcactcgt aatccgcaca acgcagttag
     1621 agctactatc gacggcttaa aacaacttaa acagatgaag cagatttaat ttctctgcgg
     1681 attgtatagg tagaaaatgg caaagacatt tattgtaaca ttaaaaagat ctcttattgg
     1741 ctgcactcaa gatcaaaaag acacggtaag atgtcttggc cttaaaaaaa ttcgtcagag
     1801 tgctgaagtg aatgacacta aggctaacag aggtcaaatt atgaaagttc agcacctcat
     1861 cactgttgat gttaaggggt aatttatgag tcttttaagt tcacttgctc caaaagtagg
     1921 ttctaaacat gccccaaaaa gggtcggtcg tggtattggc tctggtttgg gtggaactgc
     1981 cgctaagggt cacaaaggcc aattagctcg tacaggcggc aaagtacgtc gtggttttga
     2041 gggaggacag actcccttga tgagacgtct tcctaaattc ggctttagta acgttgcgtt
     2101 cgccacagaa tatgacattg taaatcttgg ccaattggat aaactttcgg gtgaagtaac
     2161 gccagaagtt ctttatcaac atggtcttat aggaaaaggt ttagttaaag ttcttggcaa
     2221 tggagagctt aagaaagctt tgaccgtgaa ggcacacaag ttcagtgaag gtgctaaaaa
     2281 ggcaatcgaa gctgctggtg gtaaggtaga ggtaattaaa tagtgtcagc cgtagatagc
     2341 atcgcaaaaa attctgatct tagaaatcgc atttttttca cgttagcaat gctagcggtg
     2401 tatcgcattg gagttcatgt acctactcct ggtgtagatg gtggcgctgt cctctctttt
     2461 ttcaatgccc aaagcagtgg aatttttgga ttatttaata ctttcacagg tggagccctt
     2521 tcccagttta gcatctttgc tcttggaatc atgccttata tttcagcttc tattattttt
     2581 caattgttga cttctgcagt gccttatttg gaatcgttaa aaaaagaagg tcaacaagga
     2641 accaaaaaaa taaatcaata tactcgttat gcgacagtag tattggctgt aattcaaggt
     2701 tatggaatta gttcgtggtt aatgaattca acaagtccta cgggtcaatc ccttgtgatt
     2761 gctccattcg ttggatttct tccattccaa gtta
//
LOCUS       DATBTE010000421         3045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2244030, whole genome shotgun sequence.
ACCESSION   DATBTE010000421 DATBTE010000000
VERSION     DATBTE010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2244030"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..500)
                     /locus_tag="VIG33_16095"
     CDS             complement(15..500)
                     /locus_tag="VIG33_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272414.1"
                     /translation="MGHIIFLFLFTLMGSVSTASATAKSFQNLLEGKWQGFCSPQALT
                     NTGRICAYTFQKSGSGTYQCDYYKDVRCTSKDSKSITSPFQFTVSGAGEKAGKVSLIF
                     LDREDVKQEKSRFYITGDVLRVQVSEVFSAPEAKKEDLEAQGVIPFFEYTKVKTSGSK
                     S"
     gene            complement(661..1827)
                     /gene="recA"
                     /locus_tag="VIG33_16100"
     CDS             complement(661..1827)
                     /gene="recA"
                     /locus_tag="VIG33_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEY8272415.1"
                     /translation="MATPTKEKDNRDSADKSHNEKVKALELAVSTIEKQFGKGSIMRL
                     GANDSLIKDIEAVSTGALSLDIALGIGGLPKGRIVEIYGPESSGKTTIALSTIAQAQK
                     KGGVVAFVDAEHALDVNYARKLGVNTEDLLISQPDTGEQALEITETLVRSGAIDVLVV
                     DSVAALVPRAEIEGEMGDSHMGLQARLMSQALRKLTGAIARSNTLVIFINQIRMKIGV
                     MFGNPETTTGGNALKFYASVRLDVRRVGAIKSGEDVVGNRTSVKVVKNKMAPPFTKVE
                     FDLMYGEGISEAGDLLDLAATANFVEKSGAWYSYNGERMGQGRDQAKQFLKDHPEMMQ
                     ELRNKILSSHGIGKLLMSTPEVGAEGSEAEVAEDEMPKAKKSEKAEKTDKKSKH"
     gene            complement(1906..2415)
                     /locus_tag="VIG33_16105"
     CDS             complement(1906..2415)
                     /locus_tag="VIG33_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY8272416.1"
                     /translation="MSELASNDEVLTHLQELLNLLRLRKLTIGFAESCTGGLLSATLT
                     SLAGISDVFMGSVVSYSYEAKMDLLGVQASTLKSEGAVSEAVALQMAQGARKQLKVGI
                     ALAITGIAGPTGGTPDKPVGTVCFAVSGPGLAENVIRKHFSGIRTEIQEASVRFALEH
                     LVSALRVKV"
     gene            complement(2412..2918)
                     /locus_tag="VIG33_16110"
     CDS             complement(2412..2918)
                     /locus_tag="VIG33_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEY8272417.1"
                     /translation="MLEKASWFRNFLKNLATFFGVGFFPKGPGTMGTLATVPLVMLLF
                     WAGPLVYMAFVIILLPLAILSAQVYEDDKGGHDHKEIVIDEVLGFLITMTWLPMTWKS
                     MLAGFLLFRVLDIFKPFPIGYLDRKIPGGFGVVIDDVVAGIIASIVLQYIYQHTLWLG
                     EQVMVFTS"
BASE COUNT          843 a          772 c          567 g          863 t
ORIGIN      
        1 aatgaatgaa cgaactaact ctttgagccg gaggttttta ctttcgtata ctcaaagaat
       61 ggaatgacac cttgagcttc gagatcttct tttttagcct ctggcgcact aaatacctcg
      121 gaaacctgaa cacgtaaaac atccccagta atatagaatc ggctcttctc ttgcttcaca
      181 tcttctcggt ctagaaaaat gagactgact ttgccagctt tttcccccgc accactcacc
      241 gtgaattgga agggggaagt gatcgacttt gaatctttgc tagtgcaacg aacatccttg
      301 tagtagtcgc attgatacgt tccagagccg cttttttgaa aagtgtaagc acatattcgc
      361 cccgtattcg taagagcttg gggcgaacaa aaaccttgcc atttcccctc taataagttt
      421 tgaaaagact ttgctgttgc agaggccgta ctcactgagc ccattaaagt aaaaagaaat
      481 aagaagatta tatgtcccat aacccaatcc tttcttagag aaggttaata aatcttaaca
      541 gaaaccctta aataaattat tgttacctat ttataccgct ttgatctgga ctttagtgac
      601 ataaaaaaag cccacgcgtc ctcaagcgca ggctttttta ttttttaggt ctgtagaaat
      661 ctaatgttta gattttttgt cagttttttc agctttttct gacttcttgg cctttggcat
      721 ttcgtcttcc gccacttccg cttcagagcc ctctgcccct acttccggag tggacatcaa
      781 cagtttgccg attccatggg aagacaaaat tttgttacga agctcttgca tcatctctgg
      841 atgatcttta agaaattgct tcgcttgatc gcgaccttgc cccatacgtt cgccattgta
      901 agagtaccaa gcgccggatt tttccacaaa gttcgccgtt gccgctaaat ccaataagtc
      961 acccgcttca gagatcccct cgccatacat taagtcaaat tccacttttg tgaatggagg
     1021 agccatttta tttttcacaa ccttcacaga ggtgcgattt cccacgacat cctcgccact
     1081 tttgattgca cccacgcggc gaacatccaa gcgaacagag gcatagaatt tcaaggcgtt
     1141 tccacccgtt gtcgtctcag ggttgccaaa catcacaccg attttcatgc gaatttggtt
     1201 aatgaaaatc accagggtgt ttgatcttgc aatggcgcct gtgagttttc tcaaagcttg
     1261 tgacatcaaa cgagcttgaa gacccatgtg gctatcgccc atttcacctt cgatctcagc
     1321 acgcggaact aaggccgcga cagagtcgac aaccaaaaca tcaatcgcgc cggaacgaac
     1381 caaagtttct gtgatctcca gagcctgctc accagtgtct ggctgagaga tcaaaagatc
     1441 ttccgtattt acgcccaatt tacgagcata gtttacgtcc aaagcatgct cagcatcgac
     1501 gaaagcaacc actccgcctt ttttctgcgc ttgagcaatt gtagacaagg cgatggttgt
     1561 tttaccagaa gactcgggac cgtaaatttc tacgatacgc ccttttggga gcccaccaat
     1621 acccaatgca atatccaagc tcaaagcacc tgtgctgaca gcttcgatat ctttgatcag
     1681 gctgtcattt gcgcccaaac gcatgatgga gcctttgcca aattgctttt caatcgtgga
     1741 aactgctaat tccagagcct ttactttctc gttatgactc ttatctgcac tatctctgtt
     1801 atctttttct tttgtaggag ttgccatttt tccctctttc tcgctctcat ctccgaaagt
     1861 gaaccgttgt tatttatatt ttattttaaa atcttaatct taaatctaaa ccttcaccct
     1921 taaagccgaa actaaatgct ccaacgcaaa acggactgac gcttcctgaa tctccgtgcg
     1981 aatgcccgaa aaatgttttc gaatgacatt ctcagcaaga cctggaccac tcactgcgaa
     2041 gcacacagtg cctacgggtt tgtccggtgt accgccggtc ggacctgcaa ttccggtaat
     2101 cgccaaggca atacccactt tcaattgctt acgcgcccct tgcgccattt gcagagcgac
     2161 tgcttcactc accgctccct cacttttaag tgtactggct tgtactccta acaaatccat
     2221 cttggcttca taagagtaac tcactaccga gcccatgaaa acatctgaaa ttccagccag
     2281 cgatgtcaga gtcgcagaaa gcaatcctcc cgtacaactc tcagcaaaac caattgtcag
     2341 tttccgtaaa cgcagtaagt ttaacaattc ctgcagatgt gttagcactt catcattgct
     2401 tgcgagctca ctcatgaagt aaataccata acttgctcgc ccagccacaa agtgtgctga
     2461 taaatatatt gtagaacaat actggcaata attcccgcca ccacatcatc aatcacaacc
     2521 ccaaatcctc ctggaatttt tctatctaga tatcctatgg gaaagggctt gaagatgtct
     2581 agtactcgaa ataataagaa gcccgccagc attgatttcc acgtcatggg gagccatgtc
     2641 atagtgatta aaaagcctaa aacttcatca atcacgatct ctttatgatc gtgaccgccc
     2701 ttatcgtctt catagacttg agcggataaa atggccagcg gcaaaagaat aattacgaag
     2761 gccatataaa caagagggcc tgcccaaaaa aggagcataa ctaaaggaac tgttgccaaa
     2821 gtccccattg tgcccgggcc ctttggaaaa aaaccgacac caaaaaatgt ggctaaattt
     2881 ttaagaaaat tacgaaacca cgaagccttt tcaagcataa ttttcctcgc ctttggtgcc
     2941 tctcttgttg tttttcgttt tgcttccaaa atgagattcc atcatgaaaa aacagtgaaa
     3001 tcatctaagt actagaaggt acggcttact ttttagtatt gcggc
//
LOCUS       DATBTE010000422         4666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2039181, whole genome shotgun sequence.
ACCESSION   DATBTE010000422 DATBTE010000000
VERSION     DATBTE010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2039181"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VIG33_16115"
     CDS             <1..635
                     /locus_tag="VIG33_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272418.1"
                     /translation="TATALSTFSKQVLDVIKKDSPSCEILDFPKTVCAAYDRLKKADP
                     EIRKKIEALQKIANSDKDNYDWAIKNSEFIQKTFAESSISAEESKEFQDAINLKIEPD
                     WRKFDPTRAKLMKHNYQKLKPILERFQTAKQNQNQTDILFADLKSKGFYEDLLTMQSC
                     LIENKPDLPKTSKDQADLNYFAQISKSYPERKFTEKDYEDARKCESSISF"
     gene            611..982
                     /locus_tag="VIG33_16120"
     CDS             611..982
                     /locus_tag="VIG33_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272419.1"
                     /translation="MRIIYFFLIFMTSLFSTAAPQMNVFQARGLRKGKCYQEIASLNC
                     KETCTLDLFPTTQAQITLYVNDISKKDRMTMGHLFEVRFKVLNKEANEIKTLSIDSAL
                     TRDSFSAIQLSDGMVIPKSCK"
     gene            complement(1124..1390)
                     /locus_tag="VIG33_16125"
     CDS             complement(1124..1390)
                     /locus_tag="VIG33_16125"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8272420.1"
                     /translation="MPITRSKTELSAEERKKLKKLGQIIRAEREKQKLTLYGVEDKGY
                     SFYQHWQAVEKGEKNITFTTLLSICDVLGKTPAELLKDLGSPKA"
     gene            1455..1808
                     /locus_tag="VIG33_16130"
     CDS             1455..1808
                     /locus_tag="VIG33_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272421.1"
                     /translation="MRISGVDLKEVIDAGLEEYKNADLTLNLLQVLQDFSSSIDVEKS
                     YDLFCCFAQIILKSDEDAAPIIADLMKTSKTELEILGDNTFSGIGSFFDDFIKKQKLL
                     SQFKELGELEVDDKE"
     gene            1877..2158
                     /locus_tag="VIG33_16135"
     CDS             1877..2158
                     /locus_tag="VIG33_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272422.1"
                     /translation="MQDVFHINFGKKRTTLTVDSILSELMAIKLGVEPGSEDAHSAVR
                     QWLQKTVPEKLGTGRGRKNASQWTRRYLIEEIADRKISTAWTDWRLKDL"
     gene            complement(2392..3324)
                     /locus_tag="VIG33_16140"
     CDS             complement(2392..3324)
                     /locus_tag="VIG33_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272423.1"
                     /translation="MTRVGFILFFVLVAGCGTTSKKLMALPPLAETAPVQVFYSQPNL
                     KYDKVCEIEAVGNNSIGGSYTQKEDFEVMFKKEARKCGADGVIFGFMHGYQQGKVTAY
                     GTGIKISGQGAGLSGENKIKAFSLAIQSHDLAKVKELLGAVPKEQSERAPTDDELVNL
                     GLYIATLDGLTCDEKIVSLLEDEYGATVPMFNAINFYSTDKNDSNTPLCRDVMARSFP
                     KMKDRSKAIVEINNHYVALLNSSYDTKLNGKAAKFSKLLNVAARAISDSCKTSSTDPA
                     CATKGAYLDFANKSKNAGVASIKKNAKDVLAILK"
     gene            complement(3340..3978)
                     /locus_tag="VIG33_16145"
     CDS             complement(3340..3978)
                     /locus_tag="VIG33_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272424.1"
                     /translation="MNGNLNSVIVGCLLGLALTGCSDGGGSAGNNDNKNTGGTGQQQE
                     IAADNCRLKSSGDIISAKFDMDLTSHGKEVYSNLFSSYSACENSDTACLARNTAQLLS
                     AQFDMELTSYGKETYTKLFNAAKACPEADSLCSVKAGGDILLAQFDTALTSYGKEQFA
                     NLFKAYQDCSASDINCLIKARGDIVIAKFQMDLTSHGQDVYSKQINAYQACK"
     gene            complement(4362..>4666)
                     /locus_tag="VIG33_16150"
     CDS             complement(4362..>4666)
                     /locus_tag="VIG33_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272425.1"
                     /translation="NWLEETKRIPVRELIEQVRGSVLNIQSSFIDRNGKLVRESHEIL
                     SQFIATPELKNKSVNLYHVENGKPIEVISISIYKLIVAFSFVAGRKPADIQYDGEL"
BASE COUNT         1366 a          967 c          973 g         1360 t
ORIGIN      
        1 taactgctac tgcactgtcg actttttcaa aacaagttct ggatgtaata aaaaaagaca
       61 gtccctcttg tgagatctta gattttccaa agacagtatg tgctgcttat gatcgcttaa
      121 aaaaagctga tcccgagatt cgaaagaaaa tcgaagcgct tcagaaaatc gccaacagcg
      181 ataaagacaa ttatgattgg gctattaaaa attctgagtt tattcaaaag actttcgctg
      241 agagctctat ttccgctgaa gaaagtaaag aatttcaaga cgcaattaat ttaaaaatcg
      301 aacctgattg gcggaaattt gatccaactc gagctaagct tatgaagcat aattatcaaa
      361 aattaaaacc aattttggaa aggtttcaga cagcaaaaca aaaccaaaat caaaccgaca
      421 ttttatttgc tgatctaaaa agtaagggtt tttatgaaga tcttttgact atgcaaagct
      481 gccttataga aaataaacct gatctgccga aaacttcgaa agatcaagca gatcttaatt
      541 attttgcaca aatatctaaa tcttaccctg aacgaaaatt tactgagaaa gactacgagg
      601 atgctcgtaa atgcgaatca tctatttctt tttaattttt atgactagct tattttcgac
      661 agcagcacct caaatgaatg tgtttcaagc tagaggatta agaaaaggca aatgttatca
      721 ggagatagca agtctaaatt gtaaagagac gtgcactttg gacttgttcc caacgacaca
      781 agctcaaatt acattatatg ttaatgacat ttctaagaaa gatcggatga caatgggaca
      841 tttgtttgaa gttaggttta aagtattgaa caaagaagct aatgaaatta aaaccctttc
      901 tattgacagt gcattaacca gggattcttt ttctgcaatc cagttgtcag acggaatggt
      961 cattccaaaa agttgtaaat aaaccccttg ataatgagct gaaaaatttt aaagaaggct
     1021 cggatcgcga taccagagcc tttttgtttt ccggctcctg cctctgctac tcttggctac
     1081 aaacacgagc aatctgatga acctcatgtt gggcggagat ttcctaggcc ttcggggacc
     1141 cgaggtcttt taagagttca gcaggagttt ttccaaggac atcacaaata ctaagaagag
     1201 ttgtgaaggt gatgtttttt tctccttttt caaccgcctg ccaatgttga taaaaagaat
     1261 agcctttatc ttcaacgcca tagagcgtca gtttctgttt ctctctctcg gctcgaataa
     1321 tctgaccaag tttctttaac ttctttcgct cttcagcgct tagttcggtt ttgctgcgtg
     1381 taatgggcat gtcctttcaa aatatcacat acgggtgatt gtaaaaagcg taacaaagcc
     1441 gaaagggaga gaatatgcgc atttcaggtg tggatttaaa ggaagtgatc gacgccggac
     1501 tagaagaata taaaaatgcg gacctcacgc tcaaccttct tcaagtccta caagattttt
     1561 catcctcaat tgacgtcgaa aaaagttatg accttttctg ttgttttgct cagattattt
     1621 taaagagtga tgaagatgcg gctcctataa tcgcggatct tatgaaaacc tcaaagactg
     1681 aacttgagat ccttggcgac aatacattct ctgggatcgg aagcttcttt gatgacttca
     1741 taaaaaagca gaaacttctg tcgcaattta aagaactcgg cgagctagag gtcgacgaca
     1801 aagaataacg tggatattat tatatcctta agcagactga tcttactcat catgtgtttt
     1861 ctaaacggag atagagatgc aggacgtatt tcatattaat tttggcaaga aacgaacgac
     1921 actaacagtg gatagtattt tatccgaatt aatggcaatt aaacttgggg tggaaccagg
     1981 gagcgaagat gcgcattccg ccgtgagaca atggcttcag aaaaccgtcc ctgaaaagct
     2041 tgggactggc cggggaagaa aaaatgcctc tcaatggaca agacgatatc tgattgagga
     2101 gatcgcggat agaaaaatat ccaccgcttg gaccgattgg cgtcttaagg acttgtagga
     2161 ccttctacct ttagagggcc tacctattga attctgtacc agcgtgaaca atgactatat
     2221 tccgagtacc agccgcgaaa ccggaatcag attccgcctc cgtaaacagc cattgctctt
     2281 acaagattca agaaattatt cgctaaaaga tggagaaggt agggggctgg aaagagttga
     2341 atttaacaag accgcgctat gtgcggcctt atctgttcag tttgtcgcta tttacttgag
     2401 aattgccaag acgtctttgg catttttctt aatcgaagcg acgccagcgt ttttcgattt
     2461 attagcgaag tctaagtagg caccctttgt agcgcacgca ggatcagtgc tcgatgtttt
     2521 gcatgaatcc gagatcgccc gagcggctac gttcaggagc tttgaaaact tagcagcctt
     2581 tccattcaac tttgtgtcgt atgaactatt caatagcgcc acgtagtgat tattaatttc
     2641 tacgatggct tttgaacgat ccttcatttt cggaaacgat cgtgccatta catcgcggca
     2701 aagcggagta ttggaatcgt ttttatctgt agaatagaaa ttgatggcat taaacatcgg
     2761 cacagtggcg ccgtactcgt cttctaagag gctcacaatt ttctcgtcac aagtaaggcc
     2821 gtcaagcgtt gcgatgtata aacctaaatt gacgagttca tcatccgtgg gcgctctctc
     2881 actctgttct ttcggtacgg cgccaagcag ctcttttacc tttgctaaat cgtgtgattg
     2941 gatcgctaag gaaaaggctt tgatcttatt ctcaccactc aggccagcac cttgacctga
     3001 gattttgata cctgttccgt aagcggtcac ttttccttgt tggtatccgt gcatgaagcc
     3061 gaagatcacg ccgtccgcgc cgcacttcct agcttccttc ttaaacatga cctcaaagtc
     3121 ttctttttga gtatagctac cgcctattga gttattaccg acagcctcaa tttcgcaaac
     3181 tttgtcgtac ttcaagtttg gttgagagta aaaaacttga acgggtgcgg tttccgctaa
     3241 tgggggaagt gccattagct tttttgaagt cgttccgcat ccagccacca gaacaaaaaa
     3301 taaaataaaa ccaactcttg tcattttaag tctccacatc tatttacacg cctgataggc
     3361 gttgatttgt tttgaatata cgtcctgtcc atgactcgtc agatccatct gaaatttagc
     3421 gataacaata tcgccgcgtg ctttgattaa gcagttgatg tcagatgcgg agcagtcttg
     3481 gtatgccttg aagagattag cgaactgttc ttttccatag ctcgtcaaag ccgtatcgaa
     3541 ctgtgctaac aagatgtcgc cgccggcttt cacagaacac agactgtcgg cctctgggca
     3601 agctttcgcg gcattaaaaa gttttgtgta ggtttctttg ccatagctcg taagctccat
     3661 atcaaactgt gcagataaga gctgtgcagt atttcttgct aagcaagcag tatctgagtt
     3721 ttcgcaagca ctgtaagagg agaataaatt cgagtagact tcttttccat gggaggtcag
     3781 atccatatcg aattttgcgg aaatgatgtc accgctggat ttcaatctgc aattatccgc
     3841 agcgatttct tgctgttgac cggtgccgcc tgtgttttta ttatcattgt tgccagcact
     3901 gcctccgccg tcagagcagc cagtcaaagc aagaccaaga agacaaccga cgatcacaga
     3961 atttaaattt ccattcataa aagcccctgt ttcagcatta ataatgcacc gcaatatatc
     4021 agaggtattt gcaacaatga agtgtattag ttacaagctc gtctatgtgc catatcttgg
     4081 atctcaaagg tgtgcttgcg gcagaggagt gaaaaaaatt cagtctacaa aaagcataga
     4141 acccaatgat tgaccgccat acacagggcc accagcgacg cgagatcggt tagggttgga
     4201 tcgaatcttt cagcagcgat gatcttgtgg gctttaccaa ctatccctta tggttcgaaa
     4261 agactgcctt aattggcgct gatttcgatc cagtttccca taacaaaaat cggacggccc
     4321 cattttgaaa atcgagacat cagacttctg tcaatctagg gctatagttc gccatcgtat
     4381 tgaatgtcag ccggctttct ccctgctaca aaagaaaatg cgacgatcaa tttgtagata
     4441 gagatagata taacctcaat tggtttgcca ttttcaacat gatataagtt aactgattta
     4501 ttttttagct ctggagttgc gataaattgg cttaagattt cgtgggactc gcggacgagc
     4561 ttgccatttc tatcgataaa agaactttga atattcaaga cgcttcctct tacttgttcg
     4621 attaattccc tgacaggaat tcgtttggtt tcctccagcc aatttt
//
LOCUS       DATBTE010000423         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_194397, whole genome shotgun sequence.
ACCESSION   DATBTE010000423 DATBTE010000000
VERSION     DATBTE010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194397"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1567
                     /gene="rseP"
                     /locus_tag="VIG33_16155"
     CDS             <1..>1567
                     /gene="rseP"
                     /locus_tag="VIG33_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEY8272426.1"
                     /translation="FIHEFGHFIIARLCGVRVEVFSLGFGKKIFQYKKGDTNYCISII
                     PLGGYVKMFGDQPGAEIVEEDKKYSFTHKNVWQRIAVVLAGPLMNFFFAIFIFFVVAF
                     IGEDYRAARVGDVPTDSKAYTAGFRSGDKIISVNSAAVSTWEELEGKLADIHNTQAMV
                     EIEKNAGGTRSLKVDISSKPNPNILSTTPTVGEIEGLSPMSKAALIGVKKESPLAALG
                     LKTGDRIVAINGEKVLYWRELEPTLDKQNPQEALQIDIERGPTSEVSKTENMSITLAP
                     IGLPKYSFDTLKLESADLYLGAILPGTPAETAGLLAGDRILQINDTKIDKWEDVISSV
                     KAYDGKEDLKLQVLRGTEVLKMTIVPQMISQMTASGTEDKRYTIGVAPFINYAMPELV
                     KISTHSPFEATKIGMQKTWDVSVMTVMSFVRLFQGKVSPKNIGGIISIGQAASETFKI
                     GISPFLKMMGLISVNLFILNLLPIPVLDGGHLLFYTIEALKGAPISMKKMEIAQQVGL
                     ALLMSLMVFALFN"
BASE COUNT          470 a          337 c          341 g          419 t
ORIGIN      
        1 tttttatcca tgagtttgga catttcatca ttgcccgtct ctgtggcgta cgggtagaag
       61 tcttcagcct cggatttgga aaaaagattt ttcaatacaa aaagggagac actaactatt
      121 gtatctcaat tatcccttta ggcggttacg ttaagatgtt cggcgaccaa ccgggagctg
      181 aaatcgtgga agaagacaaa aaatattcgt tcacgcataa aaatgtatgg caaagaattg
      241 cggtggtact tgccggtcca ctgatgaact tcttcttcgc aatttttatt ttcttcgttg
      301 tagccttcat aggcgaagac tatcgcgctg ctcgtgtggg cgatgtccct acagattcaa
      361 aagcttatac tgcaggtttc agatctggtg ataaaattat ttccgtgaat agcgctgcag
      421 tcagcacatg ggaagaactc gaaggaaaac ttgccgacat tcataatacc caggcaatgg
      481 tagaaatcga aaaaaacgca gggggcacta ggagtctgaa ggtagatatc tcaagcaaac
      541 ccaaccctaa tatcttgagc acaactccga ctgtaggaga aattgaaggc ctttccccca
      601 tgtcaaaagc agcgttgatt ggcgtgaaaa aagaatctcc attagctgct ttgggtctta
      661 aaaccggcga tcgaatagtc gcaattaatg gggaaaaggt tctctactgg cgagagcttg
      721 aacccacttt agataagcaa aatccacaag aagctctgca aattgatata gaaagaggac
      781 ctacctccga agtatccaaa acagaaaaca tgagcatcac tttagctccc atcgggcttc
      841 ctaaatacag ttttgatact ttgaagctgg aaagcgctga tttatatttg ggagctattc
      901 ttcctgggac tcccgcagaa acggcaggac ttttggccgg cgatcgcatt ctccaaatca
      961 atgatacaaa aattgataaa tgggaagatg tgatttcttc tgtaaaagca tacgacggca
     1021 aagaggatct gaagttgcaa gttctccgtg ggactgaagt tttgaaaatg actatcgttc
     1081 cacagatgat ttcacaaatg accgccagcg gaacagaaga taaacgctac accatcggag
     1141 tcgctccatt tattaattat gccatgccag aacttgtgaa aatcagcacc cacagtccat
     1201 ttgaggccac taaaattggc atgcaaaaaa cctgggatgt ttcagtcatg acagtcatga
     1261 gctttgttcg tcttttccaa ggaaaagtct cacccaagaa cattggtgga atcatttcca
     1321 tcggtcaggc agccagtgag actttcaaaa ttggcattag ccctttcttg aaaatgatgg
     1381 ggcttatctc cgtcaatctc ttcatcctga acctcttacc aattccagtt cttgatggcg
     1441 gacatctttt gttctatacc atcgaagcct tgaaaggtgc accgatcagc atgaaaaaaa
     1501 tggagattgc tcaacaagtc ggtctggcac ttttaatgag cctgatggtc tttgcactct
     1561 tcaatga
//
LOCUS       DATBTE010000424         9203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_672025, whole genome shotgun sequence.
ACCESSION   DATBTE010000424 DATBTE010000000
VERSION     DATBTE010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9203
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672025"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1177
                     /locus_tag="VIG33_16160"
     CDS             98..1177
                     /locus_tag="VIG33_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEY8272427.1"
                     /translation="MKYPPVHYHDYLKIDQLLSAQTRRSEEMGNPAHDELLFITVHQA
                     YELWFKQILFEIDSILEIFSAYPIAEQKMSLASARIDRVISILKLIMGQIDVMETMTP
                     LDFLEFRDYLYPASGFQSVQFRLVEIKLGLKEKDRLLYNETPFYKHLPEHHQKLVLKA
                     MGENSLFELIDMWLARTPFLQNKEFDFWTSYKTAVTEMFNGDRRTVLANLQLSEEMKN
                     KNIEMIDASEKTFLSTYSEADYKKLQDEGHFHLSFKAFHGALLIHLYRDQAIFHLPFK
                     ILSSLMDLDELLTQWRYRHALMAHRMLGQKIGTGGSSGHVYLKQTTEHHKVFKDFFNL
                     STFLIPRSKVPPLPTGLETKMNFNY"
     gene            1245..3116
                     /gene="htpG"
                     /locus_tag="VIG33_16165"
     CDS             1245..3116
                     /gene="htpG"
                     /locus_tag="VIG33_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEY8272428.1"
                     /translation="MAKQTQAFNAEIKQLLDLMIHSLYSHKEIFIRELISNASDALDK
                     VKFQSLTQDNLMDPGTELMIRLDASKEAHTLKISDNGIGMSPEEVVEYIGTIARSGTK
                     RFAELNQELKERPELIGQFGVGFYSSFMVADKVTLHTQKAGTHIGTLWESKGDGTYTL
                     DEIPRAEGHGTTITLHLKTFEEAEEIQDFTDPWTLKSLVKKYSDFIAYPIRMKGEKEE
                     ETLNSQKALWLKSPSEISKEEYKEFYHHLSHDWNDPLKTIHYKAEGTLEFNALLYIPT
                     QKPWNFNTRDTEYGLSLYVKRVFIMADCKDLIPQYLRFIKGLVDSSDLSLNVSREILQ
                     QNRQVTQIRKNVVNKILGTLKDMLNKERGLYENFWKEFGSTLKEGFPGDAANKEKLQE
                     ITLFKSTAAEGSGNSWTTLEEYVARMKPQQKEIYFMTGEDLNKMMESPYLEKLKEKNY
                     EVILLADPIDEWVVEALREYKGTKLQSVTKEDLNLDTEEEKKTKEEEKKGHLERFKPL
                     MESIKETLKEQIKEVNISDRLTETPVCLVAGNNEPSAHMQRILSQMGQDQGLGGKRIL
                     EINPKHAVIEKMLSLSSDQQKLWTEVLYSQALLNEGSNLPDPKRFSKHLAELMLGKH"
     gene            3384..4781
                     /locus_tag="VIG33_16170"
     CDS             3384..4781
                     /locus_tag="VIG33_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272429.1"
                     /translation="MQTLLYKVLVAFGFLFSIRASADILFVDLNNSIGEIQTVTALAA
                     ARGEKLVITPQVDASKRIAIKKKQRQMESLVEINQALSHKKQLNKSQQAVLKKNYDKV
                     YELKIEIEELLENTPPIEQEIQETFDLARQSDQKFSSMIVSGHHSIHDENLGIINKIT
                     DNDFLKILNNNKDVVSGIKAIYLWGCYTATKDRVYFWKKGLPEVRFLAGFQLKAPGDG
                     KALNHDMLEILIRKETMLVSDNEAQNAPKNILEAISSIPNVKLSSAALFLYNYYVDSS
                     FQVVDVRNENCAPNVARMMKLHYNAVVQSYLYGHKDIPEDTSSSPLRMIYEEGQQLKH
                     CQGAEMAGFMAEYIRLLKLIKFPQVSENFFSYYKEDLAKMKDSIQSKRLPVDLEVPNS
                     SDSRPKIFEWIKKVSQFAADPKNNNHPDIILMNKMSRRLQNILGNLNCITTDWIEDYG
                     PARIKSAIDEPCAEK"
     gene            complement(4803..5249)
                     /locus_tag="VIG33_16175"
     CDS             complement(4803..5249)
                     /locus_tag="VIG33_16175"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY8272430.1"
                     /translation="MSPSGPAKTTPNSLSKLLRGMRGKKVILYFYPKDNTPGCTLEGQ
                     EFNQLYGEFKKAHAEVFGVSRDSVKSHEAFRCKFNFKFELLSDTEEELCKYFDVIKEK
                     NMYGKKVLGIERSTFILDERGKLIAEFRKIKAPGHAQEILNYIKSL"
     gene            complement(5212..6327)
                     /locus_tag="VIG33_16180"
     CDS             complement(5212..6327)
                     /locus_tag="VIG33_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272431.1"
                     /translation="MRVLEYALIMLILCASCTSQQEGASLAVQAAKPAATANAPKIST
                     PPVAAAKTADKELQKILHWFEQMDAVFREALWVIRQEKAPQGKSVFGKMQRALLIEMK
                     QKLSNKSLFKCDVYSMTRNVPGVAGFPQDAEVMHQCNKKESFIKIGDWSHPKSNELFI
                     NFRGGNLEEVLGFATSILSPRMSCKLKSHDNDIIENFSCEGFMIDFNPAKNQVLRFNQ
                     FEYQRSAKTMLYLKAEVLENLDPVRKIEVDVPLEGKISVTETVLQAPEYLAKQVPAPT
                     VPKSTPTPTVSPTITPDAISEEDIYHDRNGQEIDQQEDPEARGVREPKPLPAPQKSPF
                     ENGNALEGVIESSDQAPPAEATPQPIEPERPRENNTK"
     gene            6488..8164
                     /locus_tag="VIG33_16185"
     CDS             6488..8164
                     /locus_tag="VIG33_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY8272432.1"
                     /translation="MEKIWLKQYPKGVPTEINMQEYSSIMQVFDEACQKYSHKIAFTN
                     MDVSFTYSELHEKVQDFASFLQNELKLKKGDRIALQMPNMLQFPIALFGALRSGLTVV
                     NTNPLYTAKEMLHQFKDSEVKAIVIMANYAHLLEGIIKETKIESVVVTELAELFPFPK
                     NLIINSVLKYVKKMVPPYHLPHAYSFKQALELGHDKKPQHLECVPQDIAFLQYTGGTT
                     GVSKGAILSHSNIIANMLQICAWMTPVLKEGEEVCITPLPMYHIFSLTVNCLAFMKYG
                     AHNILITNPRDLPSLVKDMKKYHFTLMSGVNTLYNGLLNNDEFRKLDFSKLKMSVAGA
                     MALQTSVAMRWKELTHSLLVEGYGLTETSPVACCNPIDGRDKVGTIGFPLPSTDVKLV
                     DDNDNEVAPGQAGEICVSGPQVMQGYWKRPDETAKVLKKGWLHTGDVALVDSDGYFKI
                     VDRKKDMILVSGFNVYPNEVEDAIASHPGVLEVAAIGIPNEHSGEAVKVFIVKKDPNL
                     TVQDVLGYAKESLTNYKVPREVEFRTELPKTNVGKILRRALRDQENPTAK"
     gene            complement(8219..8635)
                     /locus_tag="VIG33_16190"
     CDS             complement(8219..8635)
                     /locus_tag="VIG33_16190"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HEY8272433.1"
                     /translation="MEPNTSRLLNRRRFFINVGQMLGLAALAPLLGTKAFAEEKRKAR
                     PAEGGATTGADLPMVVPGKGAAAAVHYQLKHSDVKDASLKIERGGVPFEKQLCNNCSF
                     YTKHSTKNGEEVGKCQIFPNQLVKSTAWCATWNKKV"
     gene            complement(8722..9114)
                     /locus_tag="VIG33_16195"
     CDS             complement(8722..9114)
                     /locus_tag="VIG33_16195"
                     /inference="COORDINATES: protein motif:HMM:NF015180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HEY8272434.1"
                     /translation="MNPITTHETKKWDRTGVLLSALCALHCLVAPFIAYSLPLWIYSI
                     HYSPFHLFISIFIVPVAIYSFWTGYKKHKNKMVLFLGGLGIFLLSVALIGPSNRHQLR
                     WNDIMTIIGSIFLIAGHLLNRVWNRRSL"
BASE COUNT         2711 a         2088 c         1920 g         2484 t
ORIGIN      
        1 acctttgagt gccgctggat gaccagttgg gatacagaaa tcaccgatat cgaaggtttt
       61 acacacagcc ttaaggagct acacagtgag ttatacagtg aaataccctc cagttcatta
      121 ccatgactat ttaaagatcg atcaactttt atccgcgcaa actcggcgca gtgaagaaat
      181 gggaaatccc gctcacgatg agctgctttt tatcaccgtt caccaagctt acgaattgtg
      241 gtttaagcaa atactttttg aaatcgattc gattttagag atcttctctg cctatcccat
      301 tgcggaacaa aagatgtccc tggcttccgc tcgcatcgat cgggtgattt ccattttaaa
      361 actgatcatg ggccagatcg atgtgatgga gaccatgacc cccctggatt tcttagaatt
      421 ccgtgattat ctctatccag cctcaggttt ccaaagcgtt cagtttcgtt tggtggaaat
      481 aaaactcggc ctgaaagaga aagaccgttt actgtacaat gaaactccat tttacaaaca
      541 tcttcccgag catcaccaaa aactagtgct caaagccatg ggcgaaaact ccctctttga
      601 gctgattgat atgtggctgg cgcgaactcc tttcctgcaa aacaaagaat tcgacttttg
      661 gacttcctat aaaacggctg tcacagaaat gtttaatggc gaccgcagga cggtcctggc
      721 aaaccttcag ttaagtgaag aaatgaaaaa taaaaacatc gaaatgatcg atgcttcaga
      781 gaaaaccttt ctttccacct acagcgaagc ggattacaag aagcttcagg atgagggaca
      841 ctttcatctc tccttcaagg ctttccatgg ggctttgctg attcaccttt atcgtgatca
      901 agccattttc catctgccat ttaaaatttt aagctctttg atggatttgg atgagttgct
      961 cacgcagtgg cgttatcgcc atgctctgat ggctcaccgt atgctcggac aaaagattgg
     1021 aacgggaggc tcttcaggcc atgtttatct caaacaaacc actgagcatc ataaagtgtt
     1081 taaagacttc ttcaacttga gcactttcct gatcccgcgc tcaaaagtcc cgcctttgcc
     1141 tacaggttta gaaacaaaaa tgaattttaa ttattaatca ctttacttaa taattttaat
     1201 taacaacttt aattaacaac ttcaccaacc aaggaggttt atttatggca aaacaaaccc
     1261 aagcctttaa tgctgaaatc aaacaacttt tggatctgat gatccactcc ctttactctc
     1321 acaaagaaat ctttattcgt gaacttatct ccaacgcttc ggacgccttg gataaggtaa
     1381 aatttcaatc cctgacccag gacaacttga tggatccagg cacggaattg atgatccgac
     1441 ttgatgccag taaagaggcg catactttaa aaatcagcga caatgggatc ggtatgagcc
     1501 ctgaagaggt ggtcgagtac atcggcacta ttgcccgttc cgggacaaaa cgttttgcgg
     1561 aactcaatca agaattaaaa gaaagacccg aactgatcgg tcaattcgga gtgggctttt
     1621 attcttcttt tatggtggcg gataaagtga ctctgcatac gcaaaaagcc ggaacccata
     1681 tcggcacact ttgggaaagc aaaggcgacg gcacttacac cctcgacgag atcccgcgcg
     1741 ccgaaggtca tggcaccacc atcactttgc atttgaaaac ctttgaagag gcggaagaga
     1801 ttcaagactt caccgatcct tggactttga aatctttagt gaaaaaatac tccgacttta
     1861 tcgcttatcc catccgcatg aagggtgaaa aggaagagga gactttaaat agccaaaaag
     1921 ctctgtggct taaatctcct tcagaaatca gcaaagagga atataaagaa ttctatcacc
     1981 atttgagtca cgactggaac gatcctctga agacgattca ttacaaggct gaagggaccc
     2041 tggagttcaa tgctctgctt tacattccta cgcagaagcc ttggaatttc aacacccgcg
     2101 acaccgagta tggtctcagc ctttacgtca aacgggtctt cattatggcc gactgtaagg
     2161 atttgatccc tcagtatttg cgtttcatca aaggcttggt cgatagctct gatttgtcac
     2221 tcaatgtgtc tcgggaaatt ctgcaacaaa atcgtcaagt cacgcaaatc cgcaaaaatg
     2281 tcgtcaacaa gatcttggga accctcaaag acatgctcaa taaggaacgc gggctttatg
     2341 aaaatttctg gaaagaattc ggctctactt tgaaagaagg tttcccaggg gatgccgcca
     2401 acaaagaaaa gctgcaagaa atcacacttt tcaaaagcac tgcagccgag ggctccggca
     2461 actcttggac cactcttgaa gaatatgtcg ctcgcatgaa gcctcagcaa aaagaaatct
     2521 acttcatgac cggcgaggat cttaataaga tgatggaaag tccttattta gaaaaactca
     2581 aagagaaaaa ttacgaagtg attttactcg cggaccccat tgatgaatgg gtggtggaag
     2641 cccttcgtga atacaaaggc acaaagcttc aatcagtgac taaagaagat ttgaacctcg
     2701 acacagaaga agaaaagaaa accaaagaag aagagaaaaa aggccatctg gaaagattca
     2761 agcctttgat ggaatcgatc aaggaaaccc tcaaagagca aatcaaagag gtcaacatct
     2821 cggatcgtct caccgaaact ccggtttgcc tggtggctgg aaataacgaa ccttcagcac
     2881 atatgcaaag aatcctgtcc caaatgggtc aagatcaagg tttaggcgga aagcgcattt
     2941 tagagatcaa tcccaagcat gcggtgattg aaaaaatgtt gagcttgtcc tcagatcagc
     3001 aaaaattatg gactgaagtt ctttactcac aagccttgct caatgagggg tctaacctcc
     3061 cggaccctaa gagattcagc aaacatcttg ccgagctcat gttaggcaaa cactagagaa
     3121 ctctagaaat agaactcaca cagaagcctc ctggctacaa tggtcaggag gcctgcaatt
     3181 tggtgctaaa ttattagact gaataactca tagacgtcgg ctaacaaatt ttgtccttct
     3241 tggattgcac caccctcaaa tcaaattaaa cgccgcacgc ccctccgtaa aaattgcaag
     3301 ccaagcatgg atctaaatca ataattgagg tttatggatg gacttcatag gttaacaatg
     3361 aaacgggagc cgttgctatg aagatgcaaa ctctattata caaagtcctt gtcgcttttg
     3421 gctttctttt ttcaattcgg gcctccgcag acatcctttt cgtggatctt aacaactcaa
     3481 ttggggaaat ccaaactgtc actgctctag cggccgcacg cggggaaaaa ctcgtcatta
     3541 cgcctcaggt agacgcaagt aaacgtattg ccattaagaa aaaacaaaga caaatggaaa
     3601 gtctggtcga aattaatcaa gcgctttccc acaaaaaaca attgaacaaa tcccagcaag
     3661 cggtccttaa aaagaattat gacaaagtct atgaattgaa aatagaaatt gaggagcttt
     3721 tggaaaatac tcctcctatt gagcaagaaa ttcaagagac ctttgatctt gcaagacaga
     3781 gcgatcagaa attctcatcc atgattgtgt cagggcatca ttccatccac gatgaaaatc
     3841 tcggtattat aaataaaata actgacaatg attttctgaa aattctgaat aacaataaag
     3901 atgtcgtttc aggaatcaaa gctatttatc tgtggggctg ctatacagcc accaaagaca
     3961 gagtctattt ttggaaaaaa ggtttaccag aggtccgatt tctcgcgggc tttcaactca
     4021 aagcacccgg tgatggcaag gctttgaacc atgatatgct cgagatttta atacgtaaag
     4081 aaaccatgct cgtttcggat aatgaggccc aaaatgcgcc taaaaacatt cttgaagcca
     4141 tttcttcaat tcccaacgta aagctctcaa gtgcggccct ctttctttat aattattatg
     4201 ttgatagttc atttcaagtc gtggatgtca gaaatgaaaa ttgcgctccc aatgtcgccc
     4261 gtatgatgaa gctccattat aatgccgtag ttcaaagcta tttatatggc cataaagata
     4321 ttccagagga tacgtcttca tctcctctca gaatgattta tgaagaagga cagcaactta
     4381 aacattgtca aggtgccgag atggctggat ttatggcgga atatatccga cttttaaagc
     4441 tgataaaatt tcctcaggtc agcgagaatt ttttcagcta ttacaaagaa gatttagcaa
     4501 aaatgaaaga cagtatacag tctaaaagac ttcccgtcga tttagaagtt ccaaactcct
     4561 ccgattcgag acctaaaatt tttgagtgga tcaagaaagt ctctcaattt gcggctgatc
     4621 caaaaaataa caatcatcct gacatcatcc tgatgaataa aatgagccga cgcctgcaga
     4681 atatcttagg aaacctaaac tgcatcacga cagattggat cgaagactat ggaccggcaa
     4741 ggatcaagtc cgcgatcgat gaaccctgcg ctgaaaagta atctgagacg ggatcaatcg
     4801 gctcagagcg atttgatata atttaggatt tcctgggcgt gacccggagc tttgatcttg
     4861 cgaaactcgg caatgagctt gcctctttca tccaggatga aagtactgcg ttcaatgccc
     4921 aagacttttt tgccgtacat atttttttcc ttgatcacat caaaatattt gcaaagttcc
     4981 tcttcagtat ccgagagcag ttcaaacttg aaattaaatt tacatctgaa ggcctcatgg
     5041 gattttacgc tgtccctgga aactccaaaa acttcggcat gggctttttt aaattcccca
     5101 tacaactgat tgaactcctg gccctccaaa gtacaaccgg gggtattgtc cttggggtaa
     5161 aaatacagaa tcactttttt gccgcgcata cccctgagca atttgctcaa actatttggt
     5221 gttgttttcg cggggccgct cgggctcaat gggttgagga gtggcttccg ctgggggagc
     5281 ttggtcgctg ctttcaataa cgccttcaag ggcgttgccg ttttcaaagg ggcttttttg
     5341 cggtgctggg aggggctttg gctcgcggac accgcgggct tcagggtctt cttgctgatc
     5401 gatttcttgg ccattcctgt catgataaat gtcctcttcg ctgatagcgt ctggagtaat
     5461 agttggagat acggtagggg ttggagttga ttttggcaca gttggagctg gcacttgttt
     5521 tgccaaatat tccggggctt gcaaaactgt ctcggtcaca ctgatctttc cttcgagagg
     5581 aacatccact tctatcttac gaaccggatc taagttttcc aaaacttcgg ctttaagata
     5641 gagcatcgtc ttggccgaac gttgatactc gaattgatta aaacgcaaaa cttgattttt
     5701 tgcaggattg aaatcaatca tgaacccctc acaactaaag ttttcgataa tatcattatc
     5761 atgggatttc aacttgcaac tcattcttgg gctcaggatg gaagttgcaa aacccaaaac
     5821 ctcttcaaga tttcccccgc gaaaattaat aaataattcg ttggatttag gatgactcca
     5881 atccccgatt tttatgaagc tttctttttt attgcactga tgcatcactt ccgcatcttg
     5941 tggaaagccc gccacgcccg gcacatttct cgtcattgaa taaacgtcgc atttaaaaag
     6001 agatttgttc gacagcttct gcttcatttc aatcaaaagc gctcgctgca tttttccaaa
     6061 aactgatttt ccctgcggtg ctttttcttg acgaatgacc caaagcgctt ctctaaaaac
     6121 cgcatccatc tgttcgaacc agtgcaaaat cttttgcagc tctttgtcag cagtcttagc
     6181 cgccgccacc ggaggcgtag atatttttgg agcattggct gtggctgcgg gtttagcggc
     6241 ctgcacagct aatgaagctc cttcttgctg cgaagtacag cttgcacata aaataagcat
     6301 aatcagagcg tattctaaaa cgcgcatcct ttacttccct ttaaaaatcc gcctcaagtt
     6361 tgcttcaagt tctctttgac ttactgaggc taaattctct aaactttcaa tatactttca
     6421 atttagcttt aactttagca tacagcttaa ttcgcactct ctttttaaac ctataactgg
     6481 aggccctatg gaaaaaatct ggcttaaaca atatcccaaa ggggtcccta cagaaataaa
     6541 tatgcaagag tactcctcca tcatgcaagt tttcgatgag gcctgccaga agtattctca
     6601 caaaatagcc ttcacgaata tggatgtgag cttcacttac tctgaacttc atgaaaaggt
     6661 ccaagatttc gcctccttct tgcaaaatga gctcaagtta aaaaaaggcg accgtattgc
     6721 gctgcaaatg cccaatatgc ttcaattccc gatagctctt tttggtgcgc ttcgttctgg
     6781 actaacggtg gtaaacacca atcctctcta cacggccaaa gaaatgcttc atcaattcaa
     6841 ggattccgaa gttaaagcca ttgtgattat ggcgaattat gctcacttac ttgaaggcat
     6901 tattaaagaa acgaaaattg agtccgtagt ggtcaccgag ttggcggaac tgttcccttt
     6961 ccctaaaaat cttatcatca actccgtgct caaatacgtg aagaaaatgg ttccgcctta
     7021 ccacctcccc catgcttaca gctttaaaca ggccctcgag cttggacacg acaagaaacc
     7081 gcaacacctt gagtgtgttc cccaagacat tgcctttttg cagtacactg gaggaaccac
     7141 aggcgtttcc aaaggggcga tactcagtca cagtaatatt attgccaata tgttgcagat
     7201 ttgcgcctgg atgactccgg ttttgaaaga gggcgaagag gtttgcatca ctccccttcc
     7261 aatgtaccat atcttctcac tcacggtgaa ttgcctggcc ttcatgaaat atggagctca
     7321 caatatttta atcaccaatc cgcgggatct tccatccttg gttaaggaca tgaagaagta
     7381 tcatttcact ctaatgtcag gagtgaatac tttatacaat ggccttctga acaatgacga
     7441 gtttagaaag ttggattttt ccaaattaaa aatgagcgtc gctggggcca tggctttgca
     7501 aacctctgta gccatgcgct ggaaggagct cacccatagc cttcttgttg agggctacgg
     7561 actcacagaa acgtctccgg tggcctgctg caatcccatt gatggccgtg acaaagtggg
     7621 caccattggc tttccgcttc ccagcacgga cgttaaactt gtggatgaca acgacaacga
     7681 agtggctccc ggccaagcgg gtgaaatctg tgtctcgggg cctcaggtca tgcaaggtta
     7741 ctggaagcgc cctgatgaga ccgcaaaggt tttaaagaag ggttggcttc acaccggcga
     7801 cgtcgccctt gtcgacagcg atgggtattt taaaattgtg gatcgcaaaa aagacatgat
     7861 cttggtttca ggatttaacg tctatcctaa tgaggtggaa gatgccatcg cttcacatcc
     7921 tggcgtgctt gaagtggccg ccattggtat ccccaacgaa cattcgggtg aggcggtaaa
     7981 agtattcata gtgaaaaagg atccgaacct aacggttcaa gatgtccttg gatatgcgaa
     8041 ggagagtctt accaactaca aggtcccacg ggaagtggag ttcagaaccg aactccctaa
     8101 aaccaatgtg ggtaaaattt tgcgccgggc tttgcgcgac caagagaatc ctacagcgaa
     8161 atagtccatc acttcgccct ctatgcgctt taattatcaa caatatgggg ggccagtttc
     8221 aaaccttttt attccaagtc gcacaccaag cggtgctttt taccaattgg tttgggaaaa
     8281 tttggcactt acccacttct tcaccgtttt tagtgctgtg ttttgtataa aaactgcagt
     8341 tattgcagag ttgcttttca aaaggcacac cacctctttc gatttttaat gaagcatctt
     8401 ttacatcgct gtgtttgagt tgatagtgca cagctgctgc agcgccttta cctggtacta
     8461 ccatgggaag atcggcaccg gttgttgctc cgccctctgc aggacgcgct tttctttttt
     8521 cctcagcgaa agcttttgtc cccaacaaag gcgcaagagc cgcgagtcct agcatttgac
     8581 ctacatttat gaaaaaacga cggcgattta aaagacgact ggtatttggt tccattcttt
     8641 agcctccaag aataatagag aaatacaata caggtttgta tttacaaaaa ctgcaccttt
     8701 ttcaattcgt ttgcaagtag tttacaggct gcgacgattc cagactcggt tcaataaatg
     8761 tccagcaatc aagaaaatac ttccgataat cgtcatgatg tcattccagc gcagttgatg
     8821 gcgattgctg ggcccgatga gagccacgct taaaagaaaa attcctagtc cccctaagaa
     8881 taaaaccatt ttatttttat gttttttata ccctgtccaa aaggaataga tcgccacggg
     8941 aacaataaaa atagaaatga ataaatggaa gggcgaatag tgaatggaat aaatccataa
     9001 gggcaagctg taggcaataa aaggtgccac caaacaatga agcgcacaca gagctgaaag
     9061 aaggacgccc gtacggtccc attttttagt ttcatgagtg gttatgggat tcattggcat
     9121 ccctccttat ggctccaagt gcaaatgaat gtcaataaag acccccgaaa gacaaggtaa
     9181 aaccttgtct ttccccacca act
//
LOCUS       DATBTE010000425         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2381954, whole genome shotgun sequence.
ACCESSION   DATBTE010000425 DATBTE010000000
VERSION     DATBTE010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2381954"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            399..560
                     /locus_tag="VIG33_16200"
     CDS             399..560
                     /locus_tag="VIG33_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272435.1"
                     /translation="MDLLSRLNKQAVELWQKHVSCKNLEERDQLLKQYKAVFNQYVKH
                     KEWLKALES"
     gene            complement(569..1147)
                     /locus_tag="VIG33_16205"
     CDS             complement(569..1147)
                     /locus_tag="VIG33_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272436.1"
                     /translation="MIDAKAHPIKLLKIFISLAGNDSIFEFGKYIYKPGEFQNKRHVY
                     EVKASEAEEWFTSMISQLKDDEELAFQSKVKTKDNKIYHIPMIDFLSKIKKSDIQSVE
                     TVFKEFGISNFEIYETGRSYHLYGLSLISAEKFQEYMGALLLINPPDSPYPVDARWVG
                     HQLINGYTALRWTHNSSLYKKYPHKISISDLD"
     gene            complement(1147..>2006)
                     /locus_tag="VIG33_16210"
     CDS             complement(1147..>2006)
                     /locus_tag="VIG33_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272437.1"
                     /translation="SAYLKYLSDSCIPDAGKFFKKKGYFKVESETVKSVRASLGITSD
                     KFRNPLTFLMEAADDTAYCVSDIEDGIEKGIIRWEDVLSFVSNDLRNETEAVNIWNGF
                     VEKANSVKYEKSRFIDLKIRVIGACTDFVADEYIKLHDKIMDGKLDTPLYNKSSSEAK
                     ILDSLTRFARSKLFMSKQACDLELCSYNIIRGLLDHFKPLLFVQAREFDSIIDDTDSG
                     YQLEKRLALLLPKKHILAYKHDRNKFGPDVEPFLRAHLLVDYISGMTDNHALHVFQLL
                     SGISTKVRA"
BASE COUNT          622 a          376 c          351 g          657 t
ORIGIN      
        1 ctgttgaagc aactgctctt tactaagacc accgatagtg atttctttga atatattgaa
       61 cttattcgaa gacatcgttt ctaccattct accaatttgc ttcggttttc taccaattgc
      121 ggatttcaga aataaaaaaa accaccgttg tttccggtgg ttaatggtag ggagtacagg
      181 attcgaacct gcgacgtcca ccttgtaagg gtggcgctct accaactgaa taattctgat
      241 ttttatatag tgttaaagat gacgtgacaa cgtcttaagg tgggatgttg cgttattagt
      301 attatacaaa aaaatctgac tttcggttca gggtgagacc gattcctgct ctgttgatgc
      361 tccataagcc gttaattagc gttgcgggtg gaggtaatat ggatttacta agcagattaa
      421 ataaacaggc tgtcgaattg tggcaaaaac acgtctcttg caaaaatctt gaggaacgag
      481 atcagctttt gaaacagtac aaggcggtat tcaatcaata tgtgaagcac aaagaatggc
      541 taaaggcgct ggaatcttga tggtaaattt aatcaaggtc agaaattgat attttgtggg
      601 gatatttttt ataaagactg gagttatgtg tccatcttaa agctgtatac ccattaatca
      661 gttgatgtcc cacccatcga gcatctactg gatatggtga gtctggaggg ttaataagaa
      721 gcaaagcccc catatattct tgaaattttt cagctgaaat taacgacagc ccatacaaat
      781 ggtatgatct tcccgtttca tatatctcaa aattcgatat accaaattct ttgaagacag
      841 tttcaacgga ttgaatatca gattttttta tttttgataa aaaatcgatc attggaatat
      901 gataaatttt gttatcctta gtcttcactt ttgattgaaa tgcgagttct tcatcatctt
      961 ttagctgact tatcatagag gtaaaccact cctccgcttc agaggctttt acttcataaa
     1021 catgtctctt attttggaat tctccaggtt tatatatgta tttaccaaac tcaaaaatac
     1081 tatcattacc agcgagtgag ataaaaatct tcaacaactt aattggatgt gcttttgcat
     1141 caatcattaa gctctcactt tggttgaaat tccactgaga agctgaaaca cgtgaagtgc
     1201 gtgattgtca gtcatgccag aaatataatc gaccagtaaa tgtgctcgaa ggaacggctc
     1261 aacatcaggt ccaaacttat ttctgtcatg cttataagca agaatatgtt tttttggcaa
     1321 caatagcgcc aatctctttt ctaactgata tccagaatcc gtatcatcga taattgaatc
     1381 aaattctctc gcttgaacaa aaagcagtgg cttaaaatga tctaaaagac cacgaatgat
     1441 gttgtatgag cataattcca aatcacacgc ctgttttgac ataaatagct tacttcttgc
     1501 aaatctagtt aaagaatcta ggatttttgc ctcagacgaa gatttattgt aaagtggagt
     1561 gtctaacttc ccatccataa ttttatcatg tagtttaata tattcatcag caacaaaatc
     1621 agtacatgct ccaatgaccc ttattttcaa atctataaat cgagattttt catatttaac
     1681 tgagtttgct ttttcgacaa agccattcca aatgtttact gcttcagttt catttctaag
     1741 atcgttgctt acaaaactaa ggacatcttc ccatcgaatt attccctttt cgattccgtc
     1801 ttcgatatca cttacgcagt aggcagtgtc atcagcggct tccatcaaaa acgttaacgg
     1861 atttcgaaat ttgtcagatg taattcctag actagctcta acagatttta ctgtctcaga
     1921 ttctacttta aaatatccct tctttttaaa gaattttcca gcatcgggaa tacaagaatc
     1981 ggaaagatat ttcaaatagg ccgaca
//
LOCUS       DATBTE010000426         9063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2174769, whole genome shotgun sequence.
ACCESSION   DATBTE010000426 DATBTE010000000
VERSION     DATBTE010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 9063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9063
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2174769"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..1454
                     /locus_tag="VIG33_16215"
     CDS             312..1454
                     /locus_tag="VIG33_16215"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY8272438.1"
                     /translation="MNIMKDDANNHSPSPYPAASGPLEIVNADWKSEAGPLKIYLNDK
                     TISEIMVNRWDRIFIERNGMIVEAEHHFQNAEALARLVRALAVTVGKELNRRFPYLDA
                     RLPDGSRLNLVVPPISLEGPTITIRKANESVINYHELVQKGSIDGKAIYFLNRAVYAK
                     QNIVISGGTGSGKTTLLNILSSFVGDHERIVTLEDTAELKLNVKNIVRMETKPATGAD
                     PAITMDSLLKNALRMRPDRIVIGECRGAEAMDMLMAMNTGHEGSLTTVHANSAADALR
                     RLESMVLRSGIEAPLSMIQMDIGNTLNLIVQVNRTFDGKRQVVEILEVNGREKDAYLT
                     REIFKYDHEKGLLSTGAIPRFVMESRDEKLALSADIFDPGKKVRLT"
     gene            complement(1482..2987)
                     /locus_tag="VIG33_16220"
     CDS             complement(1482..2987)
                     /locus_tag="VIG33_16220"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEY8272439.1"
                     /translation="MRFKSWLESFLINKEPKNKSANTGYVYYLGTSEDPKGKTKFLSL
                     TEEHALSWQLTELKKNEREMVFFVGVKGPVWIVRPRRKLDSVQHQGLLEESDYAFSRD
                     LFGGLLGHFKAHALEQIRIEYIDTDFEPELGSLVGLGMSSYNFRQIHELKPQNWPEVF
                     LLKSSGDFDKSTLHQAQARARSVNIARHLVNLPPNELNPKTFVDFAKKLGLPVSMKIA
                     VWDAARLKKEKMGLLLAVGQGAEFGPYLLHLKYRPAKKTSLKPMAFVGKGVTFDSGGL
                     DIKPSAAMRLMKKDMGGAASVLALASWVADIKYPGPCDFYIALAENAVDAKAFRPSDV
                     FIARNGLSVEIDNTDAEGRLILADALDVAASQTGANEPEAVINVATLTGAIKVGLGAE
                     IAGLFSNDDELADSLNTAGYRAGDLNWRMPLYNKYFSEMSSPFADFKNSGGGFGGAIT
                     AALFLQKFVRGKKWAHLDIYSWTDKAQGALSAQGGNGQPVQALIEYLESRI"
     gene            3045..4064
                     /locus_tag="VIG33_16225"
     CDS             3045..4064
                     /locus_tag="VIG33_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272440.1"
                     /translation="MTWKETLNLKFCFLNAENLFLLFDEAPTPETIKANLRLDETKWQ
                     RLSSSTFENKPLKKCFELAKTLKEINADIIMLCEVGGSESLKNFNLLFLGDAYSPILT
                     EGNSNRNIDVGFLIRKNLPFYFDLQSNKNRLINYLYPHERESVKASVKSHKFSRDVAE
                     LRLFKKNVEKPFLGILLTHLKSRLDPEGIDPGGFERRQAELLALLEIYKEFSEKHPQT
                     PLMAAGDFNGNASSANTDEEFKPLYERTELKDVLELMGLSLENRATFYQVRSNNKTEG
                     RQIDFCFLSNLLAKHLKEKSAFVYRYKDEFGMGLGIPMTLDAKLRLPSDHYPIVFEIE
                     NLEIW"
     gene            4105..4494
                     /gene="apaG"
                     /locus_tag="VIG33_16230"
     CDS             4105..4494
                     /gene="apaG"
                     /locus_tag="VIG33_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEY8272441.1"
                     /translation="MPIQKTRTLHIQVAATSFYVSAESRPEQNYHFFSYKISIKNTGS
                     CTAQLMSRHWIITDGQGRVEEVRGPGVVGLQPKINPGQTFEYESACPLNTSSATMKGH
                     YQMVSEEGHSFTVEIPEFYLIAPQALH"
     gene            4649..5152
                     /locus_tag="VIG33_16235"
     CDS             4649..5152
                     /locus_tag="VIG33_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272442.1"
                     /translation="MRLPITLFTIALASTGIARAASSQPIIDLNALQASPLEISEGLA
                     PLDRSDRDGKPPKEMPQSCKDAAITPEQKTKIDEAVYQANREKIQQDADLKLAFMNYS
                     HTVKDATSDLQAGQNASTQLRDAIQRIVGSHLGLGTRILYEIVTPEQRAKTFECMLEL
                     HKKHRKF"
     gene            5322..5618
                     /locus_tag="VIG33_16240"
     CDS             5322..5618
                     /locus_tag="VIG33_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272443.1"
                     /translation="MPGKSTVSQRGRPKSIVSQAGEVVREVFDTITGTEGPRRRVRRS
                     GVRKAAARKASATKRSQPAVKRAKAIVSNIKSAGKKRGPKARSRTASTAARVHH"
     gene            complement(5693..7522)
                     /locus_tag="VIG33_16245"
     CDS             complement(5693..7522)
                     /locus_tag="VIG33_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272444.1"
                     /translation="MKNKKYFTKIPLAFIKLSLVSSCFLAQGAMATTDEWEVKDLSGL
                     KLEILKRPVKKTSPLPKLEPSTGPVTPEFPADLTVTPVSDPLKTNWFPWSPSGVNNTL
                     TSNICSQEALRDHLWSSTSAAVQARAVNDWLLRCNKEIAGPFSRHTYLTLMKFATVSY
                     DFHENPNIRMVRATLPDGRVLSGIIAMKPDDTPRPFIIAKCGIFCNSEQSTTQRFFMM
                     HLFDETPFHVLALASNTGSDFQVENKAFSLGGFDEGRQLFEIAQLVRSSASPIRKRIS
                     SVHVVGASLGGNAALYAGLYASANIIPPQEGIQSVTATCPVVVLENSARRLFSAQPIS
                     TLASFETIHHLRGIYSFVPVLGQYFPPNMRWLKGKKLFDRLMKAVVAYYHDWTAKKPW
                     DLKPFTGLQIDSLEQFWHLNDFRHYVSQVTVPTMTISADNDGFITSNLNSQLLNQTLH
                     KASNNNVGNIFLKQGNHCAFAIANGWGNYSMILREYILSHSPEAKDYWKEIQLSLPNQ
                     GFSLSSNEKILGASWMAHEGDDFLILQLKVSGGFHIIDRELNIPIPIKTLPWDFTAIP
                     QSSYEVGLLTRFANTRFSVVDEKGELVANSNHAPKYIKAWVWR"
     gene            complement(7642..>9063)
                     /locus_tag="VIG33_16250"
     CDS             complement(7642..>9063)
                     /locus_tag="VIG33_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8272445.1"
                     /translation="YDNQIQVLDNHTLGKLVKVHIQKENEAPILASLMSNSSIEYVVP
                     NFKLHAFSAPMEPSVLKAQWAIAKVQVEKAWQRAGNKGNKNVLVAVIDTGVDYTHDSL
                     RPNMVSGFDFAEDDSDPMDKTSAQNPGHGTHCAGVIGATGIIEGGTVGVSPEVSIMPL
                     RFLTENGSGDLNNAIKAIDYAIAHNVQVISASWGASVSRSQAAPLLEAVKRADDAGII
                     FVVAASNDGKNNDSTEVYPANNGYPNSISVAASGPNDEKPSWSNFGTAKVHLASPGLN
                     ITSTLSGNKYGDLSGTSMATPLVAGLVALLKAQDPSLTGSQIRAILQTTGVKAQIQTA
                     CNCRVDAYAAIDAVLNKQMTLVPAAATIATGEKIGFQVWNGTAPFSFVSSNPSVADVS
                     ASGELVGSSNGTTIVTVTDSTGKTAATLDINVGAKPSNPGNPGNPGNPGNPGDPGSCP
                     VGDPQMCQIICQIQPDLPFCTKR"
BASE COUNT         2578 a         2190 c         1936 g         2359 t
ORIGIN      
        1 cgttggtaaa aacgtgggtg ctaacagcgt aagtaatttc atttattcct ccaagaatat
       61 ggttattact tttcaagaag atcatcataa tcatcctttc agtgttatac aacgggacaa
      121 actgatggtc caaagccagt aaaaattatt tcacagttct tattcgtcct cccctttctt
      181 ttctccttga taatagatga tcgagtaggt ccagttatca ttaaactgga cctaaccata
      241 aagggtccat ctaaggaaac taaaactatt gtgcaaaaga actgttaagt taatcctaca
      301 atttccgatt tatgaacatc atgaaagacg atgccaataa ccactctcca tctccttatc
      361 ccgctgcctc aggacccctt gagatcgtta atgctgattg gaaatccgaa gctggacctc
      421 tcaagattta tctcaatgat aaaaccatct ctgaaattat ggtgaatcgc tgggaccgga
      481 tctttatcga acgcaatggc atgatcgtgg aagccgaaca tcactttcaa aacgccgagg
      541 ctttggcccg cctggttcgc gctttagccg tcactgtggg taaagaactc aatcgccgtt
      601 tcccctattt ggatgcgcgg ctgccggatg gctctcgctt gaatcttgtg gtacccccta
      661 tttcccttga aggccccaca attactattc gaaaagccaa cgagagcgtg atcaattatc
      721 atgaactcgt gcaaaaagga tccatcgacg gcaaagctat ttactttcta aaccgcgccg
      781 tctatgcgaa acagaatatt gtcatcagcg gaggaaccgg gagcggcaag accactcttc
      841 tcaatatcct cagttctttt gtgggtgatc atgaacgcat tgtcacctta gaagacaccg
      901 ccgagcttaa attgaacgtc aaaaatatcg ttcgcatgga aaccaaacct gcaaccgggg
      961 ctgacccggc cataactatg gatagtctgc ttaaaaatgc cctgcgaatg cgtcccgacc
     1021 gtatcgtgat tggagaatgt cgaggtgctg aagccatgga tatgctgatg gccatgaata
     1081 cgggccatga aggaagtctc accaccgtcc acgcgaactc cgctgcggat gcccttcgcc
     1141 gtctggaatc gatggtgcta cgttctggaa tcgaagcccc gctctccatg attcaaatgg
     1201 acattggaaa tacccttaat ctgatcgtgc aagtaaatcg tacttttgat ggcaaacgtc
     1261 aggtggtcga aatcctcgaa gtgaatggcc gagaaaaaga tgcctatctc acgcgagaaa
     1321 tctttaaata cgaccatgaa aaaggtctgc tttccacggg ggcgatcccc cgcttcgtga
     1381 tggaaagccg cgatgaaaaa ctcgctctca gcgcggatat ttttgatcct ggaaaaaagg
     1441 tccgactgac ctaaatcact ggcctattca gaatttagat tctagattct agattctaga
     1501 tattcaatca aagcttgtac aggttgaccg ttgccccctt gagcgctcaa ggcaccttga
     1561 gccttatccg tccagctgta aatatccaag tgagcccatt tcttcccacg cacaaatttt
     1621 tgtaaaaaaa gagcggcggt aatagcgccg ccaaaacctc cgccagagtt tttgaaatcc
     1681 gcaaaaggcg aagacatctc actgaagtac ttattgtaca aaggcatgcg ccaattcaag
     1741 tctcctgctc tgtaaccggc ggtgttgagc gagtccgcga gctcatcatc attgctaaaa
     1801 agcccggcga tttccgcacc gagacctact ttaatggcgc cggttaaagt ggccacatta
     1861 atcaccgctt cgggctcatt tgctcctgtt tggctggcag cgacatccaa ggcatcagcg
     1921 agaattaatc gcccttccgc atcagtatta tcaatttcca cgctgaggcc attgcgagca
     1981 ataaagacat cactaggacg gaaggctttc gcatccaccg cgttttcagc caaagcaata
     2041 taaaaatcgc aaggacctgg atactttata tccgccaccc aagaagccaa agccagaaca
     2101 ctggcagcgc cacccatatc ttttttcatg agcctcattg cggcggaagg cttgatgtcc
     2161 aagccacccg aatcaaaagt gaccccctta cccacaaagg ccatcggctt taaagaagtc
     2221 tttttggcag gtcggtattt gaggtgcagc aagtaaggac caaactcggc cccttgcccc
     2281 acagccaaca gcaatcccat tttttctttt ttcaaacgag ccgcatccca cacagctatt
     2341 ttcatagaaa cgggaagacc cagctttttg gcaaaatcca cgaaagtttt tggattcaat
     2401 tcattgggtg gcaaattcac caaatgccga gcgatattca cggacctcgc acgagcttga
     2461 gcctgatgga gcgtgctttt atcaaagtct cctgagcttt ttaaaagaaa aacttctggc
     2521 caattttgag gttttaactc atgaatttga cggaaattat aagaactcat tccaagtccg
     2581 accaaagacc ccaattcggg ttcaaaatcc gtatcaatat actcaatacg tatttgttca
     2641 agagcatggg ctttaaaatg ccccaaaagg ccgccgaaca aatcacgact aaacgcatag
     2701 tcagattctt ccaaaagacc ctgatgttgt accgaatcca gctttctgcg agggcggaca
     2761 atccaaacag gtcctttaac ccctacaaag aacaccattt cacgttcatt tttcttgagt
     2821 tccgtcaatt gccaactcaa agcgtgctct tccgtcaagg aaagaaattt agtttttcct
     2881 ttggggtcct cactggtgcc taaatagtaa acataaccag tgttagcact tttattttta
     2941 ggttccttat taattagaaa actctccaac caagatttaa atcgcatgtg tcacccctag
     3001 atttagtttg agattctgcc atgatttttc ttacaatgta agttatgaca tggaaagaga
     3061 ctctaaatct caaattttgt ttcctcaacg cagaaaacct ctttctgcta tttgatgagg
     3121 ctcccacgcc ggaaacgatc aaagccaacc tcaggctgga tgaaacgaaa tggcaacgtc
     3181 tttcttcgtc gacctttgag aacaaacctt taaagaagtg tttcgaactg gctaagactt
     3241 tgaaagagat caacgccgat atcatcatgc tctgtgaagt cggaggctca gaatctctga
     3301 aaaacttcaa cttgctcttc ctgggagacg cttactcccc tattttgact gaagggaact
     3361 caaaccggaa tatcgatgtg ggttttttga ttcgcaagaa cctgcccttt tacttcgatc
     3421 tgcaatccaa taaaaatcga cttatcaatt atctttatcc ccatgaacga gaatcggtga
     3481 aggcttcagt caagagtcat aaattttctc gcgatgtggc agaacttcgt ttatttaaga
     3541 aaaacgtcga aaaacctttt ttagggattt tactgactca cttaaaatca cgcctcgatc
     3601 cggaaggaat tgaccccgga ggtttcgagc gccgacaagc ggaactcttg gctttacttg
     3661 aaatctacaa agagttttcg gaaaaacatc cacaaactcc cctgatggcg gcgggtgatt
     3721 tcaacggaaa tgcgagcagc gccaataccg atgaagaatt taaaccgctc tacgaaagaa
     3781 cggaacttaa agacgttctt gaactaatgg gtcttagttt ggaaaataga gcgacttttt
     3841 atcaagtgcg ctctaacaac aaaacggaag gtcggcagat tgacttctgc tttctttcca
     3901 acttactagc aaaacatctc aaagaaaaaa gcgccttcgt ctaccgatat aaagatgagt
     3961 ttggcatggg cttgggtatt cctatgacgt tggatgccaa actcaggctt ccatcggatc
     4021 attatccgat tgtttttgaa atcgaaaatt tggaaatctg gtgattgacc aaaagggaca
     4081 aaagctctta atcttgttta agttatgccc attcaaaaaa ccaggaccct tcatattcaa
     4141 gtggccgcga cttcctttta cgtctcggca gagtcccgcc cggaacaaaa ttatcatttc
     4201 ttttcttata aaataagtat caaaaatacc ggctcttgca cggctcaatt gatgagtcgt
     4261 cattggatca tcactgatgg gcaaggacgc gtagaagaag tgcgagggcc tggggtcgtc
     4321 gggttacagc caaaaatcaa tcctggccaa acttttgaat atgaaagcgc ctgtcctttg
     4381 aatacctctt cggcaacaat gaagggccac tatcagatgg tcagcgaaga aggccatagc
     4441 ttcacagtgg aaattccaga attctatttg attgctccgc aagctttgca ttaaattgct
     4501 cggttgattt tttattttgc cctcaactga cacagcatga accgatctgg atggaaaaaa
     4561 cattaaaaac acgtccgacc ttccacttaa actcacattc actcattacg ttctccttac
     4621 tttgaaactt acaaccaagg agagatttat gagattacca attacacttt ttacgattgc
     4681 actggcttcc acaggcattg cgcgcgctgc cagctctcaa ccaataatag atttgaatgc
     4741 cttgcaagca agtccgctag agatttcgga agggctcgct ccattagatc gcagtgatag
     4801 agatggaaaa ccccccaaag aaatgcccca atcttgtaag gacgccgcga tcacccctga
     4861 acaaaaaacc aaaattgatg aagccgtcta ccaagcaaat cgcgaaaaaa ttcaacaaga
     4921 cgcggacctt aaacttgcct tcatgaacta cagccatacc gtgaaggatg caacgagtga
     4981 tctccaggcg gggcagaacg ccagcactca actcagggac gccattcaaa gaatagtggg
     5041 atctcacttg ggcttaggca ctcgcatttt gtatgaaatc gttactccag agcaaagagc
     5101 taaaactttc gaatgcatgt tagagcttca taagaaacac agaaaattct aaatcactga
     5161 atcccccttt tcctgaaccc acatcacttc acggagatgt gggttttttt atattatatc
     5221 ttcacaattg tgaagatatc ttcatttgca tcttcacctc tttctgttgc ctgattaaat
     5281 ccaacactaa cttaatgtta gtttttttaa aggaggtaaa tatgccaggt aaatccacgg
     5341 tgagccaaag aggccgccct aagtcaattg tgtctcaagc cggagaagtg gtgcgtgaag
     5401 tgtttgatac cattacgggc acagaagggc cccgcaggag ggtgcgtcgc tcaggagtgc
     5461 gcaaagccgc agcgagaaaa gccagtgcca caaaaagaag ccagccagcc gtaaaacgag
     5521 ctaaagcgat agtgagcaat ataaaaagcg caggaaaaaa acgtgggcct aaagctcgct
     5581 cccgtactgc ctcgaccgcg gcacgagtcc atcattaatt tcaagaaact gtgcgcggtt
     5641 tcagcctcat taaaccgcgc ccgtttctct cagtgctgaa tcgcttatgt ctttatctcc
     5701 aaacccaggc cttaatatat tttggcgcat gattggaatt cgccactaac tctccttttt
     5761 catccacaac gctgaaacga gtatttgcaa agcgagtcag gagaccgacc tcataactcg
     5821 attgcggaat tgcggtgaaa tcccaaggaa gtgtctttat cggaatgggg atattgagtt
     5881 ctcgatcgat gatgtggaag ccgccggaaa ccttcagttg gagtataaga aagtcgtctc
     5941 cctcatgagc catccaactg gctcccaata ttttttcatt tgaagaaaga gagaacccct
     6001 gattcggcaa ggacaactga atttctttcc agtaatcttt agcctcaggg ctatggctta
     6061 aaatgtattc ccgtaaaatc atggagtaat ttccccagcc gttggcaatc gcgaaggcac
     6121 aatgattccc ctgcttcaaa aagatattgc ccacattatt gttggaggct ttgtgtaagg
     6181 tttgattgag cagttgtgag tttaggttgg aggttatgaa accatcattg tccgccgaaa
     6241 tggtcatcgt agggacagtc acttgagaaa catagtggcg gaagtcgtta agatgccaga
     6301 actgctccaa cgaatcaatc tgaagcccgg taaagggctt cagatcccag ggcttttttg
     6361 ccgtccaatc atgataataa gccaccaccg ctttcatgag cctatcgaag agtttcttgc
     6421 ctttaagcca acgcatgtta gggggaaagt attgacctaa cactggcaca aaagaataaa
     6481 ttccgcgaag atgatgaatg gtctcaaaac tggctaaggt tgaaatcggt tgcgctgaaa
     6541 ataaacgcct tgctgagttc tccagaacca ccaccggaca agttgcggtg acactctgaa
     6601 tgccttcctg aggagggatg atgttggcgc tggcataaag tcccgcatac aaggcagcat
     6661 ttccgcccaa actggcaccg accacatgca cgctggagat tcgcttccga atcggagaag
     6721 ccgatgatcg caccaattga gctatttcaa aaagttgccg tccttcatca aatcccccta
     6781 gcgaaaaggc tttattttcg acctgaaaat ccgagcctgt gttactggcc aatgccaaaa
     6841 catgaaaggg cgtctcatca aaaagatgca tcataaaaaa acgctgtgtg gtgctctgct
     6901 cgctgttaca aaatattccg cattttgcga tgataaaagg gcgaggcgta tcatcgggct
     6961 tcatcgcaat aatgccactc aacacccgtc catccggaag cgtggccctg accatgcgga
     7021 tattcgggtt ctcgtgaaag tcataagaga ccgtcgcgaa cttcatcagc gtgagataag
     7081 tatgccttga aaaaggaccg gctatttctt tattacatcg gaggagccaa tcattcacag
     7141 ctcttgcctg aactgctgcc gaggtgctgc tccacaggtg atcccggaga gcctcctgac
     7201 tgcagatgtt gctggtcaaa gtgttattca ctccgctagg ggaccacgga aaccaattgg
     7261 tttttagggg atcgctgaca ggagtcaccg tcagatctgc cggaaactcc ggagtcactg
     7321 gccccgtgga aggttctagt ttcggcaaag gagaagtttt ctttacagga cgcttcagga
     7381 tttcaagctt caggccggaa agatctttta cttcccactc atcagtggta gccatcgctc
     7441 cctgagctag aaagcaggaa gacacaaggg aaagcttgat aaaagcgaga gggatcttag
     7501 taaagtattt tttatttttc atagaaatac gtactagcac tctttatttt gtatgaacaa
     7561 tccgtcttct atgaataaaa cgtcaaaaaa aaaggcttcg tttccgaagc cttttcatca
     7621 tttacaattt tttcttccaa tttaacgttt agtacaaaat ggaagatctg gttggatttg
     7681 gcaaataatt tggcacattt gtggatcccc cactgggcag cttcctggat ctcctgggtt
     7741 tccaggatta cctggatttc cagggttccc agggttgctt ggtttcgcgc ccacattgat
     7801 atccaaagtg gcagcggttt ttcccgtaga atcagtcacc gtcacaattg tagttccgtt
     7861 gcttgagccg accaactcac cgctggcact cacgtccgct acggaaggat tgctgctgac
     7921 aaaactgaaa ggagcggtcc cgttccaaac ttgaaaacca attttttctc cagtagcaat
     7981 agttgccgct gctggaacca aagtcatttg cttgttcaaa accgcatcaa tagctgcata
     8041 ggcatctaca cggcaattac aagctgtttg aatttgcgct ttaacaccgg ttgtttgcaa
     8101 gatagctcga atttgagatc ctgttaaaga aggatcctga gctttcaaca aagcgaccaa
     8161 gccagccacc agtggagttg ccatggaagt tccagacaaa tcaccatatt tattaccaga
     8221 taaggtgctg gtgatattca aaccaggaga cgccaagtga acctttgcag ttccgaagtt
     8281 tgaccaagat ggtttctcat cgttagggcc agacgccgca acagaaatgg aatttggata
     8341 tccattgttc gccggataaa cctcagtgct gtcgttgttt tttccatcgt tggaagcggc
     8401 aaccacaaag ataatacccg catcatctgc acgcttcact gcctccaata aaggagccgc
     8461 ttgtgagcga gagaccgaag ctccccaaga agcagaaatc acttgcacgt tgtgggcaat
     8521 cgcgtaatca attgctttaa tcgcgttgtt caaatcgccg gaaccgtttt ctgttaagaa
     8581 ccgaagtggc atgattgaaa cctcgggact cacacccacg gtaccccctt caataatgcc
     8641 tgtcgctccg atcacacctg cacagtgggt tccgtgacct ggattttgcg cgctcgtctt
     8701 atccatggga tcactgtcgt cctcagcaaa gtcaaaacca gaaaccatat ttggtctcaa
     8761 agaatcgtga gtatagtcaa cgcctgtatc aatgaccgcg acgagaacgt ttttgttccc
     8821 tttgttgccg gctctttgcc aagctttttc aacttggact ttcgcaatcg cccattgtgc
     8881 ctttaaaaca gagggttcca taggagctga aaaagcatgc aacttaaaat tcggcaccac
     8941 atattcaata gatgaattgc tcatcaatga agcaagaata ggagcttcat tttccttttg
     9001 aatgtgaacc ttaaccaact tccccagagt gtggttatcc aagacctgaa tctgattatc
     9061 ata
//
LOCUS       DATBTE010000427         7847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_878706, whole genome shotgun sequence.
ACCESSION   DATBTE010000427 DATBTE010000000
VERSION     DATBTE010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878706"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VIG33_16255"
     CDS             <1..854
                     /locus_tag="VIG33_16255"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY8272446.1"
                     /translation="QSLESPRAQARAQQKPFDLEHVSRFDLLFAILKSTPLKEIKEDY
                     FGKDHFRNKAFFEAYFSNYIEGTTFEIAEAEEIIFDHKIPEDRPKDAHDILGTFTIIS
                     DPNEMKKTPQSYSELDALIKQRHYTLMQNRPEVLPGKYKEKPNRAGDTHFVHPNNIQG
                     TLEQGFQRYQNLPPGLARATFMMFLISEVHPFSDGNGRIARIMMNAELYATKHSTIII
                     PNVYREDYLTALRALTRRDRPDPLIKMLSRAHRFSHLDFSNYKKALHEIEMKNWFRDS
                     SEARLIE"
     gene            1025..1732
                     /locus_tag="VIG33_16260"
     CDS             1025..1732
                     /locus_tag="VIG33_16260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272447.1"
                     /translation="MTLLKKAAQYFSALCLLASFNARAADAICDSTNFETQKLTNKDR
                     SHQTMMGVRPTYSAKLGEGFSHSDKSGLLLKENKIVPWGAGDILSVRPQNSDEQTKKG
                     IVKPVKFFVQYDRPPYNNVNDKRTKGGIYKVDSKVQNTDGTGVSCSTEPNDYAYHYFP
                     DADEKSLVVGSVYCVRTRSGNEYTLIRVTHICDNGVVFDYKYNGDSNVFEGVKKVPRA
                     APHESKPTAPGSHPGKS"
     gene            complement(1742..3055)
                     /locus_tag="VIG33_16265"
     CDS             complement(1742..3055)
                     /locus_tag="VIG33_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY8272448.1"
                     /translation="MVIWKLRTGADKRVRGGHPWVFSNELASSPKGIGLGAFVELQDA
                     RGEFLARGYGNPHSLISFRAVSFNAEEREPLGFRALSKKVLHSWQVRKNAGFRGSFRM
                     SFGEADFLPGLVLDYYLIEQNGKQGQVFVAQLVTAGLDKALQDAEVFFKLLVEEAQKL
                     SLSKLSWEETAVVIRNDVNVRKLEGLEIQEPRTLKSIDGMGLEKVDILLNSASDEGVI
                     RMQCDLAKGQKTGFFLDQTQNIYQAVKLFRNWAKAHPELKSQPIKILDLCCYVGHWAT
                     QTTRALKQLGFEVEVHLADVSESALQFAKLNAEREGAKVTVHKVDVLEGLDKFEGRSF
                     DIIVADPPAFIKAKKDIPTGRHAYLKLNTQAFRMIKPQRFVVSCSCSGLLEEEDFKEA
                     IRKAIRRNNWQARNLTRGGHAADHPTLLQFPEGFYLKMFTHFVEA"
     gene            complement(3055..4155)
                     /locus_tag="VIG33_16270"
     CDS             complement(3055..4155)
                     /locus_tag="VIG33_16270"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEY8272449.1"
                     /translation="MAKLSIPFLVLSLLLMVNSSLAKSLSPKLQLELYAQTSFAPKLT
                     FNKEEVGGLSGIYWDGMKLTAISDNRGKFGPPRFYEMDLKISAEPKITIAVTKVRHLK
                     DTPKDWILDLEALVPLPDGDLLVSTEGDNNHKPRAMPHVFVISSAGVYKNDISLPDKY
                     LPEATGLQKKGIENNRGFEGMTINSKDQNLYVMNEYPIIADQGDDDLNLWLRLVEFKK
                     GKASFKVNAEFPYVVNKGAKNGAGVEAFRGVSELLSVNDSQLLVLERGARLTKTGIAY
                     SGGLYVVDITGAKDVSAVKNLSEGKAEPLKKEMLIDFEEVLKNQKVENFEGLTWGPTL
                     ADGRKSLLVISDNNFAAKEKTTLLVFAVKEVE"
     gene            complement(4143..5183)
                     /locus_tag="VIG33_16275"
     CDS             complement(4143..5183)
                     /locus_tag="VIG33_16275"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY8272450.1"
                     /translation="MQSTFLRNVWYVGLPTRELEKNKMQSRVILGEPIVFYRDSQNQV
                     SALRDICPHRGIPLSYGRVVEDKVECPYHGWKFDNTGTCTEIPSLCEGQDLDCRKIKV
                     LHYPVREQQGLIWVFIGDKNFDLSKTPEIPFMKNLPADVQPRVLEKCIFPCHIDHAVI
                     GLMDPAHGPYVHKSWFWRSEKTTYEKRKMFAPVTHGFQMVRHQPSSNSKAYKILGGVP
                     TTEITFSLPCVRVEHVQVGKRNLYSYTALTPVSEKQTIIHQMVYWDSPWLNLMRPVIR
                     KFAQIFLHQDMDAVSKQQDGLNFDPSLMLIKDADTQAKWYYALKAEWKNHFDEKREFV
                     NPVKSIELRWRS"
     gene            complement(5196..6056)
                     /gene="folD"
                     /locus_tag="VIG33_16280"
     CDS             complement(5196..6056)
                     /gene="folD"
                     /locus_tag="VIG33_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEY8272451.1"
                     /translation="MILLDGKKVAAVRRDALKPRIESFKKATGRAPHLAVILVGEDPA
                     SQVYVRNKHRSCQNTGIDSSVHVVPANINQDTLLQWIEKLNNDDKVDGILVQMPLPKH
                     LSLEPVLRNIMLEKDADGLTYANAGYLMVGKPFIKPCTPMGIMSILEYFKINVSGMDA
                     AVVGRSQIVGKPMAMLLNEANATVTLCHSKTRNLREHTSRADLVVVAAGQKRLLGKED
                     FKKGAVVIDVGMHGTGAGELCGDVRFEELGDIVAAITPVPGGVGPMTITSLLENTVFL
                     AEVRAGLAHS"
     gene            6218..6502
                     /locus_tag="VIG33_16285"
     CDS             6218..6502
                     /locus_tag="VIG33_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272452.1"
                     /translation="MAEKVKLSKDGSSIDFVQSDGIPTSLKKFRQSPEIEGFYRFVFE
                     NDLQKESYEILDQIIIARKAKKSLAAKEAKAAAKELDAASKNPKAKLKKK"
     gene            6642..6893
                     /locus_tag="VIG33_16290"
     CDS             6642..6893
                     /locus_tag="VIG33_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272453.1"
                     /translation="MLKNKKGQSLIEYLIIVAIVAIGTMSIVRVVGKNVSVQFANVAK
                     ALGSGDDSQLKAEKIDSKMYSKKDLSNFLDGATSSDSKK"
     gene            6877..7218
                     /locus_tag="VIG33_16295"
     CDS             6877..7218
                     /locus_tag="VIG33_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272454.1"
                     /translation="MTLKNNKGQALIESLALFITLMATLSVFIGILYFGLVHVGMNYL
                     LHEFLVCKVAHEEKLCHREFEQRAKAFLFAAQVTRFESSRHFNKQKVRVTLSMPMNRR
                     LSMQKELELYP"
     gene            7215..>7847
                     /locus_tag="VIG33_16300"
     CDS             7215..>7847
                     /locus_tag="VIG33_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272455.1"
                     /translation="MNSQKGFALIGLISLLPVILAGGLAVFSTFGFLKSDLGTLNACR
                     AKQLQIQNKAGKNLSKLLKLNPRALKLRIQQAHAEKALAAAIESGQPPAIAAAEAYLL
                     KIQMQRQVLAIKQKVLIQTADIWLASGGADLPKELQREWSQHNTPLKSWIQSSLRLTA
                     AKVPKLAVQADIPEVAPVYQLLPHFEEAQSWEQTWNVEFRTVSWAQKFLNF"
BASE COUNT         2221 a         1833 c         1631 g         2162 t
ORIGIN      
        1 ctcaatcctt agagtcacca agagcgcagg ccagagccca gcaaaaacct tttgaccttg
       61 aacacgtcag ccgctttgat ctgctttttg ccatcctcaa atcaacgcca ttgaaagaaa
      121 tcaaagagga ttacttcggg aaagatcact ttcgcaacaa agctttcttt gaagcctatt
      181 tctcaaatta tattgaaggc acgacctttg aaattgcaga agctgaagag atcatatttg
      241 accataaaat cccggaagat cgtccaaagg atgctcatga tattttaggg actttcacca
      301 taatctctga tcctaatgag atgaagaaaa ctccgcaaag ttattcggag ctggatgcat
      361 tgatcaagca acgccactac actttaatgc aaaatcggcc ggaagttcta ccaggaaaat
      421 ataaagaaaa acccaatcgt gcgggggata cgcacttcgt tcatccaaat aatattcagg
      481 gaacactgga acaaggtttc caaagatacc aaaatctacc gccgggactg gcacgcgcga
      541 cattcatgat gtttttgatt tcagaagttc accccttcag cgatggcaac ggacgaattg
      601 cacgaatcat gatgaatgca gaactttatg cgactaagca ttccaccatt atcattccta
      661 atgtgtatcg cgaagactac ctcactgccc ttcgcgcact cacgcgtcgt gatcgtccgg
      721 atcccttaat taaaatgctc tcgcgtgcgc acagattttc ccatttagac ttttcaaatt
      781 ataaaaaagc ccttcatgaa attgaaatga aaaactggtt ccgcgactct agtgaagcaa
      841 ggctgatcga atagtattat aaagagtccc gggtgtttgc tgtatacatg gaagccatta
      901 gaagcttctg caaaatccat gactagacga agatatagct gctgactgat atctgtaaac
      961 aattctcgca tggcataatt tttaaagtaa taatgaataa attttcaaat taaccggaga
     1021 atttatgact cttttaaaaa aagctgctca gtatttttca gctttatgcc ttttagcttc
     1081 gttcaatgcg cgagctgctg atgctatttg tgatagtacc aattttgaaa ctcaaaaact
     1141 gactaataaa gatcgcagtc accaaaccat gatgggtgtg aggcctactt attcggccaa
     1201 attaggagag ggttttagtc atagcgacaa aagcggtttg ctattaaaag aaaataaaat
     1261 tgttccttgg ggggccgggg atattttgtc tgttcgtcca caaaattctg acgaacagac
     1321 aaagaaaggc atcgtgaaac ctgtcaaatt ctttgttcaa tacgatcgac ctccttataa
     1381 taatgttaac gacaaaagaa ctaagggagg catctataaa gtagattcca aagttcagaa
     1441 cactgatggc acgggagttt catgctcaac agaaccaaat gattatgctt atcattattt
     1501 tcctgatgct gatgaaaaat ctctagtcgt aggaagtgtt tattgtgttc gcactcgctc
     1561 aggcaatgaa tacaccttaa tcagagtcac ccacatttgt gacaatggag tggtttttga
     1621 ctacaaatac aacggagact ccaatgtctt tgagggtgta aagaaagtac ctcgagccgc
     1681 tccccatgag tccaagccca ctgcgcctgg ttctcatcca ggcaaatcat agagtcctat
     1741 tctaggcttc gacaaaatga gtgaacattt tcaagtaaaa cccctcagga aattgcagga
     1801 gggtcggatg atccgctgcg tgtcctccgc gggttaagtt gcgagcctgc caattgttgc
     1861 gtcgaatggc tttgcggatg gcttctttga aatcttcttc ttccaaaaga ccagaacacg
     1921 aacaagacac cacaaatctt tgcggtttta tcatgcggaa ggcctgggta tttaatttta
     1981 aataagcatg gcgaccggta ggaatgtctt tcttcgcctt aatgaaggcg ggcggatcag
     2041 ccacgataat atcaaagctg cggccttcga atttatccag accttcgagc acatcgacct
     2101 tgtgtactgt gaccttggcg ccttcccgtt ccgcattcag ttttgcaaat tgcagggcgg
     2161 attctgaaac gtccgctaga tgaacctcca cttcgaagcc caattgtttt aatgcccgag
     2221 tggtttgcgt tgcccagtga cccacataac aacacagatc gaggattttt atgggctgcg
     2281 atttcaactc gggatgagcc ttcgcccaat ttctgaaaag ttttaccgcc tgataaatat
     2341 tttgcgtttg atcaaggaag aagcctgtct tttgcccttt ggccagatca cattgcatgc
     2401 ggatcacacc ctcatcgctg gccgagttta aaagaatatc cactttttca aggcccattc
     2461 cgtcgattga ttttaaagtc ctgggttctt ggatttccaa tccctcgagc tttctcacat
     2521 taacgtcgtt gcggatcacc acggcggttt cctcccaact caatttcgat aaactcaatt
     2581 tctgcgcttc ttcgacaagc agcttgaaga aaacttcggc gtcttgcaga gccttgtcca
     2641 atcccgcagt cacgagttgg gccacaaaga cttggccctg ttttccattt tgctcgatca
     2701 aataataatc caaaaccaaa cccggcagaa agtcggcttc gccaaaactc atacgaaaac
     2761 tgccacggaa accagcgttt tttcggactt gccaggaatg gagaactttt ttacttaaag
     2821 cccgaaaccc cagaggttca cgctcttccg cattaaaact cacggcgcgg aaggaaatca
     2881 atgaatgagg attgccataa ccacgggcca gaaattctcc gcgggcatct tgcaattcta
     2941 cgaaagcacc gaggccaatg cctttggggc tgctggccag ttcgttggag aaaacccacg
     3001 gatgcccgcc tcgaactctt ttatccgccc ctgtgcgaag tttccaaatg accattattc
     3061 cacctcttta accgcaaata cgagaagagt ggttttctct ttcgctgcga aattattatc
     3121 actgataacc aataagcttt tgcgcccgtc agccaaggtc ggaccccaag tcaaaccctc
     3181 aaaattttca actttttgat tttttaaaac ttcttcaaaa tctatgagca tttctttttt
     3241 aagaggctcc gctttccctt cggaaagatt tttaacagct gagacatctt tagctccggt
     3301 gatatcgaca acatacagcc ctccactata ggcaatgcct gtcttagtga gacgggctcc
     3361 ccgttccaag accagcagct gagagtcatt cacagagagc aattccgaaa ctccacggaa
     3421 ggcttctacc cctgctccgt tcttcgcccc tttattcacc acatagggga actccgcatt
     3481 cactttgaag gaggctttgc cttttttaaa ttccaccaaa cgcagccaga gattgagatc
     3541 atcatcacct tgatccgcaa tgattggata ctcattcatg acatataaat tctggtcctt
     3601 cgaattgatg gtcatgccct cgaagccacg attattctca atgccttttt tctgaagccc
     3661 tgtggcttct ggcaggtact tatcaggaag agaaatatcg tttttataaa ctcctgccga
     3721 agagatcaca aagacatgtg gcatcgcgcg aggtttgtgg ttgttatctc cttcagtgct
     3781 cacgagaaga tccccatcgg gcaaaggaac gagggcctcg aggtccaaaa tccaatcttt
     3841 cggtgtgtct ttcaaatgtc gaactttggt gactgctatt gtgatcttcg gctcagcaga
     3901 gattttcaaa tccatctcat agaaacgtgg gggaccaaac ttaccccgat tatcactgat
     3961 cgcagtcaac ttcatcccat cccaatatat tcctgatagc ccccccacct cttctttatt
     4021 gaaagtgagc ttcggagcga aactggtctg agcatagagc tctaactgca actttggtga
     4081 caaactcttc gccagcgaag aatttaccat taaaagcaaa cttaaaacca ggaatggaat
     4141 acttaacttc gccatctcag ctcgatggac tttacaggat ttacaaattc tcgtttttca
     4201 tcgaaatgat ttttccactc cgccttcagc gcataatacc attttgcttg cgtgtctgcg
     4261 tctttgatca acattaaact gggatcaaaa ttgagtccgt cctgttgttt cgaaaccgca
     4321 tccatatctt ggtgcaaaaa gatttgagcg aacttgcgaa tcacgggtct catcaaattc
     4381 aaccatggag agtcccaata aaccatttga tgaataatgg tttgtttctc acttaccgga
     4441 gtgagggcgg tgtaagagta aagatttctc tttcccactt gcacgtgctc gacacggaca
     4501 caaggaagac taaaggtgat ctcggtcgtc ggcactcccc ctaaaatttt gtaggccttt
     4561 gaattcgaag atggctgatg tcgcaccatc tggaaaccat gggtcaccgg tgcaaacatc
     4621 ttgcgcttct cgtaagtggt cttttcactg cgccagaacc agcttttatg cacataaggt
     4681 ccgtgggcag gatccatcaa gccaattacc gcatgatcaa tgtgacaggg gaaaatacat
     4741 ttctcgagaa cgcgcggctg aacatccgct ggcagatttt tcataaaagg aatttcggga
     4801 gttttggaaa gatcaaagtt tttatctccg atgaacaccc agattaaacc ttgttgctcg
     4861 cgcaccgggt aatgcaggac tttgattttt ctgcaatcca aatcctgacc ctcacaaagt
     4921 gaagggattt cagtacaggt ccctgtatta tcgaatttcc atccatgata aggacactcc
     4981 accttatcct ccaccactcg tccgtagctg aggggaattc cgcgatgggg acagatatca
     5041 cgaagagcgg aaacttgatt ttgagaatca cgatagaaca caataggctc tcccagaatg
     5101 acccgcgatt gcattttatt tttttctaac tccctggtgg gaaggcctac ataccaaacg
     5161 ttacgtaaaa aagttgattg cataattctc cattattatg aatgagcaag gcccgcacgc
     5221 acttcggcca aaaatacggt gttttccaaa agtgaagtga tcgtcatggg gccgacgcct
     5281 cctggaacag gagtgatcgc tgcaacaata tctcccaatt cttcaaagcg cacatcccca
     5341 cagagttcgc ccgctcctgt gccgtgcatt ccgacgtcaa taaccacggc gcctttttta
     5401 aaatcctctt tacctaagag gcgtttttgt cctgccgcta ctacaaccag atccgcacgt
     5461 gaggtgtgct cgcgtaaatt gcgagtcttt gaatggcaca aggtcacggt ggcattggct
     5521 tcatttaaaa gcatcgccat cggcttgcca acaatttgac tgcgccccac caccgcggcg
     5581 tccatacctg atacgttgat tttaaaatat tcaagaatgc tcatgattcc catgggagtg
     5641 caaggcttaa tgaaaggttt ccctaccatg agataaccag cattcgcgta tgtcagacca
     5701 tcagcatctt tttccagcat gatatttcgt aaaacaggtt ccaggctcaa atgttttggc
     5761 aaaggcattt gcacgaggat gccatccact ttgtcatcat tattaagttt ctcaatccac
     5821 tgaagaagtg tgtcctgatt aatattggcg ggaactacat gaactgagga atcaatgccg
     5881 gtgttttgac aagagcgatg cttgtttctg acgtaaactt ggcttgctgg atcttccccg
     5941 actaagatca ctgcaagatg gggagctctg ccggttgctt ttttaaatga ctcaattcta
     6001 ggtttcaaag catctctacg aactgccgcc accttttttc catccaagag gatcatcgaa
     6061 taccgccttt ttttgctctc aatttgttct atttcactaa gaaattcaat aatttatctt
     6121 taaaaaacca gaaagaacct cgcccactgc ccttatcttg accagagaga gcggctagtt
     6181 tattgtacaa attcttgaaa ctacggagga atttaaaatg gctgaaaaag ttaaactttc
     6241 taaagatgga agttctatcg actttgttca atctgatggt attccaacct cactcaaaaa
     6301 attccgccaa agccccgaaa ttgaaggctt ctatcgtttc gtctttgaaa atgatctgca
     6361 aaaagaatct tatgaaatcc tcgatcaaat tattattgcc cgtaaggcaa agaaatccct
     6421 ggcagccaaa gaagccaagg ctgcggctaa agagctggat gcagcttcaa agaatccaaa
     6481 ggctaagctt aagaaaaagt aattctttat ttgttttgtt tgaaatctag aattccgaaa
     6541 agagatccga gctacggatc tcttttttta tgtcctcgcg ataaatgctt cctcactttg
     6601 gatctcttct tgctatctcc tgcagtgcgg gaggaagtct tatgcttaaa aataaaaaag
     6661 gccaaagcct tatcgaatat ttgatcattg tggcgattgt cgccattggg accatgtcta
     6721 tcgttcgggt ggttgggaaa aatgtttcgg tgcaatttgc caatgtcgcc aaagccttgg
     6781 gaagcggcga tgattctcaa ctcaaggccg aaaaaataga ttccaaaatg tatagtaaga
     6841 aagatctcag taattttcta gatggcgcca catccagtga ctctaaaaaa taacaaaggc
     6901 caggctttga ttgaaagtct tgctcttttt ataactctga tggcgactct ttcggtgttt
     6961 ataggaattc tctattttgg cttggttcat gtaggaatga attatttgct gcatgaattc
     7021 ctggtgtgca aagtggctca cgaggaaaag ctttgccacc gtgagttcga gcaaagagcg
     7081 aaagcttttc tatttgccgc tcaagtgacg aggtttgaaa gtagccgaca cttcaataaa
     7141 caaaaagttc gcgtaacgtt aagcatgccg atgaatcgcc gtctaagtat gcaaaaagaa
     7201 cttgagcttt acccatgaat tcacaaaaag gttttgcctt aattggactt atttccctat
     7261 tgcctgtgat tctcgccgga ggtttggcag tcttcagtac gtttggattt ctaaagagtg
     7321 atcttggcac tttaaatgcc tgtcgggcca aacaattgca gattcagaat aaggcgggta
     7381 aaaatcttag caaactttta aagctcaacc ctcgggcttt aaaactgaga attcaacaag
     7441 ctcatgcgga aaaagcgtta gctgcagcga tagaaagtgg ccaaccgcca gcgatcgcag
     7501 cagcagaggc ttatctccta aaaattcaaa tgcaaaggca agttttggct attaaacaaa
     7561 aggttctaat ccagaccgct gatatatggc ttgccagcgg gggtgctgat ctcccaaaag
     7621 agctgcagcg ggaatggagt caacataaca ctccattaaa atcatggatt caaagctctt
     7681 tgcgtttaac cgccgcgaaa gttccaaaac tagcggtgca agcggatatt ccagaggtgg
     7741 caccggttta tcaactcctg cctcattttg aagaagcgca atcttgggag caaacctgga
     7801 atgtggaatt tagaacagtc tcctgggcac agaagtttct aaacttt
//
LOCUS       DATBTE010000428        13222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_878963, whole genome shotgun sequence.
ACCESSION   DATBTE010000428 DATBTE010000000
VERSION     DATBTE010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 13222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13222
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878963"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..140
                     /locus_tag="VIG33_16305"
     CDS             9..140
                     /locus_tag="VIG33_16305"
                     /inference="COORDINATES: protein motif:HMM:NF018168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HEY8272456.1"
                     /translation="MNIVQEMTDMIQTNRVFESTQKAIGAYDSMAEKLVNIVGKTSL"
     gene            163..948
                     /gene="flgG"
                     /locus_tag="VIG33_16310"
     CDS             163..948
                     /gene="flgG"
                     /locus_tag="VIG33_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEY8272457.1"
                     /translation="MLKSLNTAATGMAAQQTNMDVIANNIANVSTAGFKKSRAEFEDL
                     VYQTQKEPGTATGMNSYSPNGVQTGLGVRTAAVQKDFAAGSALVTKNPLDLQIEGSGF
                     FQVLTPDGQIGYTRDGAFKKDPNGRIVDKNGNLLQPEITVPPNVAGIEVAPSGEVRVI
                     QGMSDTPQTIGQIDVVNFVNPAGLKAMGKNIFTQTPSSGQPQTARPGLNGTGYLSQGQ
                     LESSNVNIVDEMVNMITAQRAYETNSKVIQASDQMLQSINNMR"
     gene            990..1889
                     /gene="flgA"
                     /locus_tag="VIG33_16315"
     CDS             990..1889
                     /gene="flgA"
                     /locus_tag="VIG33_16315"
                     /inference="COORDINATES: protein motif:HMM:NF024544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEY8272458.1"
                     /translation="MMASLAWARPEVIFPPETEVSNTAVISVFQVAEMKELKGLAFRE
                     IARMPLVEKIEKQENLVLSGKEVSKKLRDLVKNSELLKKINPSFKIPSEIHIHIRQEG
                     FSKIEVERSMKNILASRCDSCRFQITIDSMPKMNPLNGRIDWEQEIKYGSFMIAVREA
                     EQFVNKWITGTLHVRKTVPVAKKLIRFGERLQTEDLEILEADVSYVKEETPDLSQVVG
                     LIANRTLTPRTPILLSDLKREPAAHRGQVVRALVGDQDFEISINASAEENGFIGDLIK
                     VKNLETQKMMSGIVIDKGVVKVQ"
     gene            1886..2638
                     /locus_tag="VIG33_16320"
     CDS             1886..2638
                     /locus_tag="VIG33_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEY8272459.1"
                     /translation="MSKSRNALYFSMLGFAVVFLSGCAHLSDFLARFSGQDSEAVVTQ
                     DSDGASVRYSENPQMSVPSNRQYKRMTRSRMEEESDLGAQAGSMWVMEGQGAYLFAQN
                     KSRREGDVLNVKLDGPSLKQVETKVNIIKKLLKQLEEQEQANLNGGLAANPAEGDRAP
                     AAAKPEEKKEDGKDELVNIQQISTKIVEKQADGNYRVRGAQPFMIGKREYKVIAAGLV
                     RPEDFNDEGVSSTKLIDPQFDVVSIRRKPNNE"
     gene            2631..3623
                     /locus_tag="VIG33_16325"
     CDS             2631..3623
                     /locus_tag="VIG33_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEY8272460.1"
                     /translation="MNKILVALMITATGFSATAARLKDIASIRGVRENQLIGYGIVVG
                     LKGTGDGKSEFTSKSMVRMLDKLGMKMDSADMQSKNVAAVIVTATLPPFGKAGNPVDI
                     TVSAIGESQSLQGGTLLQTPLRAANDQVFAVAQGSIILGGDGKDIHTTAGRVPNGAII
                     ERDFSGDFASRKMFRMTLHNPDFTTAARSVLTINKELGGVFASAKDAGTIDIITPAAY
                     ENRGVELLATIESIDINPDSIAKVVINEKTGTIVIGDKVKISKVAISHGSLAVKVADG
                     KKSGDEKVAVLDGGVSVGDLVQALNKLGVSPKDLITILQSIKAAGALHGELEVL"
     gene            3937..4617
                     /locus_tag="VIG33_16330"
     CDS             3937..4617
                     /locus_tag="VIG33_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HEY8272461.1"
                     /translation="MKIPAAGPQVKTPDQKLHEVSEMYEKHFLGEMMKAMRSTVHEGG
                     FVQSNQAEKIFREQLDQEYVDKWSAQGGIGLSELIYKQMIEKYGVQMGIKNPVVKPQG
                     PLPLTEKDTFTAKSFHQNKKTENLSFRFDKKAPMAGEFNKPLPVSSPWDGILLEKKSL
                     LTGETFLQVDHDNGLRSQLVFKGVAGSLKTGQKIQAGETLGLLSPEVKTFFWHTQTGP
                     QDLETVSE"
     gene            4679..5008
                     /gene="flgM"
                     /locus_tag="VIG33_16335"
     CDS             4679..5008
                     /gene="flgM"
                     /locus_tag="VIG33_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HEY8272462.1"
                     /translation="MKITHNKIGQNLNMVDGAKTDRTKTNTSPAIGAEKLDGLEELGA
                     SKVEVSPRAQQAKRIKELAMAAPDVDMAKVAKFRQLIDEGKYKVDAKAVADRLVDEQL
                     AMDDLKN"
     gene            5053..5583
                     /locus_tag="VIG33_16340"
     CDS             5053..5583
                     /locus_tag="VIG33_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HEY8272463.1"
                     /translation="MESTIIESTTDRSYQRLVANLEEMTKLYRQLLDLVRKEKQLLVD
                     ANMEKLQENNEIKENLLFKIKSVDGLRMRYAAELATLVEADHENPRLLDIAKNLGGVE
                     GDRLRVIHSALDLLVKRLSFLNRENEDVAQTALKTLNGALGNIKDTLSGKKTYGAKGQ
                     VQRGPELAGHFVSKEG"
     gene            5637..7049
                     /gene="flgK"
                     /locus_tag="VIG33_16345"
     CDS             5637..7049
                     /gene="flgK"
                     /locus_tag="VIG33_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEY8272464.1"
                     /translation="MSKISAMLDLGKRSLMNNQTALQTVGHNIANKSTEGFSRQRVEV
                     VTNQPQGEGNLRIGMGARAGVITRVNNPWLEKQIQREGSNLGFADAKADGLNRVEQVY
                     NEQTNKGLNQYMTGFFNSFRELSNNPESLSSRTMVREASESMTKEFGRVTTQLGDVQK
                     DLDGQLMTLTEEVNLMSKEVADLNEKIQIVEAQGMPANDERDRRDLLLKKMGEKIDIS
                     WAEGKDGHISVTAGRTAILVSGTSSTKIRAAPTSDRNRVEIFYQSSPTGTPVNITNQI
                     KGGRMGGALEVRDEVIEGFLDSIDKMAYSLAEEVNKVHIEGFDRSGKPGVLFFEMPEQ
                     VKGAASTLSLNRTIANDVGRIAAGSESGAPGDNTVANVISSLQHRQVMDDGTATFDDF
                     YNTQVGQVGAVTQRAIKGQESQKNVVDQLQNIRESISGVSLDEETTKMIEFQKAYSAS
                     ARLIRVADEMFDTVLSLKRM"
     gene            7066..8154
                     /gene="flgL"
                     /locus_tag="VIG33_16350"
     CDS             7066..8154
                     /gene="flgL"
                     /locus_tag="VIG33_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HEY8272465.1"
                     /translation="MRIADKMQYNQVNQNVTKNRTEMSELQNQAATQKRLTKPSDDPL
                     ASARVLSARTEERGNQQYIKNINNVKSFLEFTDQSLGELSEILMRAKELAIGQANDAS
                     GNEQSRQVSASEIEQIYNQSVQIGNRKLGDRFIFGGSQTQTAPFDQQGQYAGNDSDMK
                     IGIHKDTFVSMNIPGNKVFLGKGLSGDGIVRSRSETPTSVKSLQDFKQEEIQRREENQ
                     DREANFVETRAPASSYRAQRVGAIAKDPVTNSGGVNLFQTLKGLEISLRANDKQGIQE
                     TLDTLDQVISQVVQARSEVGARVMAVTNTTESLQKAIVDNKVMASQLEDVELFDVVSS
                     INKTDSTLRATLETSGKLIQQSLMDFLK"
     gene            8308..9534
                     /locus_tag="VIG33_16355"
     CDS             8308..9534
                     /locus_tag="VIG33_16355"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272466.1"
                     /translation="MDRRKILKFAGLASAARIFFGKLNPASAQTWQKIANPTGSAGTW
                     QKLTIASSVNAYSISSIATGGLTHTGVSRSDGLVFTSGANNYGQVGNNTVVAKSTFTQ
                     ALGISNIAMIGAGQHHTIALRSDGLVFGCGYNTAGQVGDNTAVNKSTFVQALGISNAV
                     AVAGGYWHTVALRSDGLVFATGADWSGILGDNDNTGTNKSTFVQALGISNAVAISCGI
                     GTSVALRADGLVFACGYNDEGQLGINTVASKLTYVQALGISDAVAIAAGERFVVALRS
                     DGLVFGCGLNGHGQLGINATTNRSTFVQAIGISNITAIAGGSFHTLALRSDGLVFACG
                     YNAAGQVGDNTTANKSTFVQALGISNAIAIACEGNSSVALRSDGLIFATGNNSNGQLG
                     NNATVSKLTFVSAIAP"
     gene            9762..9989
                     /gene="csrA"
                     /locus_tag="VIG33_16360"
     CDS             9762..9989
                     /gene="csrA"
                     /locus_tag="VIG33_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HEY8272467.1"
                     /translation="MLVLTRKLGESIAIDDHIKIRVVQIKGKQVRLGIEAPKDTKIHR
                     EEVYLAIQEQNQQSATVSSDKTRSVAKLLKP"
     gene            10178..10711
                     /locus_tag="VIG33_16365"
     CDS             10178..10711
                     /locus_tag="VIG33_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HEY8272468.1"
                     /translation="MIINTSRFGRVELQNNDVLTFPEGLLGFADLRRFVLLDDPSDEI
                     FAWLQSCESSHIAFPVLEPELFVDTYKATLTKGDLEALKLQSQDNARYFTIVTIPDDP
                     TQMTANLKAPIVVNISEKMARQCVLQDNNLAIREPIFTKLQQRVVQNPATPIKNQSAG
                     MDVAIKLGNKNSSDQIL"
     gene            10812..11480
                     /locus_tag="VIG33_16370"
     CDS             10812..11480
                     /locus_tag="VIG33_16370"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8272469.1"
                     /translation="MTKRRSISVREPRKEPKQERSRQTYQAILQGAAKIIRKSGVQKF
                     STNKVAKEAGVSIGSLYQYFPSKEAILAALIDQILAYEYSYMKERFRNFSPEIGAREV
                     FKQVFSYYYITEPEELNYRKIMMDAVAWVNKTAIALKFYKSIAELIVNFTQEHFDSPQ
                     STRDNDAAVFVIQYMLRATIFSSVDKELEKINLEKLIDELAEILMGILRIPTEHRGAL
                     PEEK"
     gene            complement(11495..12658)
                     /locus_tag="VIG33_16375"
     CDS             complement(11495..12658)
                     /locus_tag="VIG33_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY8272470.1"
                     /translation="MMSLILAFLTGALMTAVGSWFLYRIKQQSFQLQNEFTARSLEQM
                     RGQQADLQLKMSQQFENMAHKIFEEKSTKFTTQNMQNLNTVLDPLKERLKDFEKKVEE
                     TYGHERAERGALRGELGKLMELNRLMSNETVNLTKALKGEAKTQGNWGELILENILER
                     SGLRKGEEYILQGTNMDLRGEDNQVLRPDVIVKLPEGKHLIVDSKMTLNAYEAYSSAT
                     SLEDQERLGKLHVEALKKHINGLSEKKYYAIDKLVSPDFVILFMPLEPAFALAFKLQP
                     DLFQSSWDKHIAIVSPTTLLTTLRTVSSLWKQDRQQKNALDIAKRGGALYDKFVGLVK
                     DIENLGDKLGAAQKAHDEVMGKLMTGKGNLIRQVEDLKSMGAKTEKSLPQIEG"
     gene            12765..>13222
                     /locus_tag="VIG33_16380"
     CDS             12765..>13222
                     /locus_tag="VIG33_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEY8272471.1"
                     /translation="MQRIKNWLIAFRPKTLTACVVPILVASALVKALHLSVDVEVVVL
                     ALFASFFIQIGTNLVNDAVDFKKGADTEKRMGPRRVTQSGLFSYKQVMLAAGLCFIFA
                     VACGIPLVIKGGTAILVIGVLSVLMGYAYTMGPIPLAYFGLGDLFVILFFG"
BASE COUNT         3820 a         2678 c         3242 g         3482 t
ORIGIN      
        1 cgagcaatgt gaacatcgtc caagaaatga cggatatgat tcaaaccaat cgggtttttg
       61 aaagtaccca aaaggcgatt ggtgcttacg acagcatggc tgaaaaatta gtgaatatcg
      121 tgggaaagac ttcgctgtaa taaagcctga aggaggtagt gaatgttgaa gagcttgaat
      181 accgcagcta caggaatggc ggcacaacaa acaaatatgg acgttattgc gaataatatc
      241 gccaatgtct ctaccgctgg ctttaagaaa tcccgtgctg aatttgaaga cctggtttat
      301 caaacgcaaa aagagccggg aaccgcaacc gggatgaatt catattctcc caatggggtt
      361 caaactggac tgggagttcg cacggccgct gtgcaaaaag attttgcggc cggaagtgct
      421 ctcgtcacaa aaaatcctct tgatttgcaa attgagggct cgggattttt tcaagtattg
      481 actcccgatg gccaaattgg ctacacccgt gacggtgcat ttaaaaaaga tcctaatggt
      541 cgcatcgtcg ataaaaatgg caatcttctc caacctgaaa tcaccgtccc tcccaacgtg
      601 gccggtatag aagtggcgcc gagtggtgaa gtgcgagtga ttcaaggcat gagcgatact
      661 ccgcaaacca ttggccaaat tgatgtcgtg aatttcgtca atcccgcagg ccttaaagcc
      721 atgggtaaaa acattttcac gcagacccca tccagtggtc agccgcaaac ggcccgccca
      781 ggattaaatg ggaccggtta tctttcacaa ggccagctgg aatccagcaa tgtgaatatc
      841 gtggatgaaa tggtgaatat gatcactgcg caaagagctt atgagaccaa cagcaaggtc
      901 atccaggcgt ccgatcaaat gctccaatcc attaataaca tgaggtaata ctagtgtgta
      961 ttttgaaagt tcttttaggg ggaatcacaa tgatggcaag cctggcatgg gctcgccctg
     1021 aagttatttt tccccctgaa acagaagttt ccaatacagc ggtgatttcc gttttccaag
     1081 tggccgaaat gaaagagctt aaaggtctgg cgtttcgtga gatcgcgagg atgccactgg
     1141 tggagaaaat agaaaaacaa gagaacctgg ttttatctgg taaagaggtc tctaaaaaac
     1201 tgagagactt ggttaaaaac agcgaacttt tgaaaaaaat taatccctct ttcaaaattc
     1261 ccagcgaaat tcatattcat attcgtcagg agggtttctc taaaattgag gttgaaagat
     1321 cgatgaagaa tattcttgcc tctcgttgtg attcttgccg ttttcaaata accattgatt
     1381 ccatgccgaa aatgaatccc ttgaatggga ggatcgattg ggagcaagaa attaaatatg
     1441 gttcttttat gattgccgtg cgtgaggcgg agcagttcgt caataaatgg attaccggaa
     1501 ctcttcatgt cagaaaaact gttccagtgg caaaaaaact gattcgcttt ggcgagagac
     1561 tccaaaccga ggatcttgaa attctggaag cagatgtcag ctatgtaaaa gaagagacgc
     1621 cagatttatc ccaggtggtg ggtttaatag cgaatcgcac gttgacccct aggaccccca
     1681 ttttgctctc ggatttgaag cgagaaccgg cggctcatcg agggcaagtg gttcgagctt
     1741 tggtaggcga ccaagatttc gaaatttcca ttaatgcttc cgccgaggaa aacggcttta
     1801 tcggagattt gattaaagtt aaaaatttag aaacacagaa aatgatgtcg ggaattgtta
     1861 ttgataaggg tgtggtaaaa gtccaatgag taaaagcagg aatgcacttt atttttctat
     1921 gctagggttc gcggtggtgt ttctttccgg ctgcgctcat ttgagtgatt tcctggcgcg
     1981 cttcagtggt caagattctg aagccgttgt cactcaagat agcgatggcg cttccgttcg
     2041 ttattctgag aatccgcaaa tgagtgtgcc ttcaaatcga caatacaaac gtatgacccg
     2101 ttcgcgaatg gaagaggaat cggaccttgg tgctcaagct ggttctatgt gggtgatgga
     2161 aggtcaagga gcctatctct ttgcacagaa taaatcacgg cgtgaaggag atgtcttgaa
     2221 tgtgaagctg gatgggcctt cgttgaaaca ggtggaaacc aaagttaaca tcattaaaaa
     2281 attactaaag cagctggagg agcaagagca agcaaatctc aatggcggtt tagcggcgaa
     2341 cccggcggaa ggcgaccggg ctccggccgc ggcaaagcct gaagagaaaa aagaagatgg
     2401 caaagatgaa ttagtcaata tccaacaaat ctccacaaag atcgtggaga aacaggccga
     2461 tggaaattat cgtgtacggg gcgcgcagcc tttcatgatt ggcaagcgtg aatataaagt
     2521 gatcgctgca gggctggttc gtcctgagga cttcaatgat gagggcgtta gctccacaaa
     2581 attgattgat ccccagttcg atgtcgtcag cataagaagg aagccaaaca atgaataaaa
     2641 ttcttgttgc cttgatgata acagcaacgg gtttttcggc aacagccgca cgactcaaag
     2701 atatcgccag tatccgaggt gtccgagaga atcaattgat cggttatgga attgtggtgg
     2761 gtcttaaagg tacgggtgac ggtaaatcgg aattcacttc aaagagtatg gtgcgcatgc
     2821 tggacaagct cggtatgaaa atggattcgg cggatatgca aagtaaaaat gtggctgcgg
     2881 tcattgtcac cgcgacactt cccccttttg gtaaagccgg aaaccccgtg gacatcactg
     2941 tgagtgcgat cggagaatcc cagagtctgc aaggaggaac tcttttgcag acgcctttaa
     3001 gggcagcgaa tgaccaagtg ttcgcagtgg ctcaggggag tattattctc ggcggtgatg
     3061 gaaaagacat tcataccaca gcgggaaggg tgccaaacgg cgcgatcatc gaacgtgatt
     3121 ttagcggaga cttcgcttcg cgcaaaatgt ttcgcatgac tttgcacaac cccgatttta
     3181 cgacagcggc gcgaagtgtg ttgactatta acaaggaact gggtggtgtt tttgcttcag
     3241 ctaaagatgc gggcacaatc gatatcatta cgcccgcagc ttatgagaat cgcggcgtgg
     3301 aattgttggc cacgattgag tccattgata tcaatccaga tagcatcgcc aaagtggtga
     3361 tcaatgaaaa gacaggaacg attgtcatag gcgataaagt aaaaatatcg aaggtggcga
     3421 tttctcatgg aagccttgcg gtgaaagtcg ccgatggaaa aaaaagtggg gatgaaaaag
     3481 tggcggtttt agatggtggt gtcagtgtcg gagatctagt ccaggcgctg aataagctcg
     3541 gagtctctcc gaaagacctg ataacaatac ttcagtccat taaggcagct ggagctttgc
     3601 acggggaact cgaagtttta tgaaaaatat gtttcgaaat gaaacagctt ttattaaact
     3661 gatattacca atttcaaatt tattggtttc agacatatgg atgtgtttga aacacgggga
     3721 gggtgaggaa gagatcgcga accatggaag gtcaagagtc gaaagtttct cgcaggaaac
     3781 aaatgcaata ggtcagcgga cgtcaaggat tgacgaacat gagccgaagc taaatgttca
     3841 tcgcaagatg ggcagctcag agataagtgc aggatgcacg gattctcaga ctcaactccc
     3901 cttttttttc tcttcacgtg atgctgggca cgcactatga aaatcccagc tgccggtccg
     3961 caagtgaaga ctccggatca gaagcttcat gaagtctctg agatgtatga aaaacatttc
     4021 ctcggcgaga tgatgaaggc gatgcgctca acagttcatg agggcggctt cgtacagagc
     4081 aatcaagccg agaaaatatt cagagaacaa cttgatcaag aatacgtgga caaatggagt
     4141 gctcaaggcg gaatcggcct gagtgaactc atttacaaac aaatgattga aaaatacggt
     4201 gtgcaaatgg gaattaaaaa tcctgtagtg aagccgcagg gcccattgcc cttgactgaa
     4261 aaagacacat tcaccgcgaa aagttttcat caaaataaaa aaacggaaaa cctctctttc
     4321 cgtttcgata aaaaagcacc aatggccgga gagttcaata agcctttgcc ggtttctagt
     4381 ccatgggacg gaattttatt agagaagaaa agcttattaa ctggcgagac ctttttacag
     4441 gtcgaccatg acaatggtct aagaagtcag ttggttttta aaggggttgc cggttcgctc
     4501 aagaccgggc aaaaaatcca agctggcgag actcttggac ttttaagccc tgaagtaaaa
     4561 actttttttt ggcataccca gactggacct caagacctag aaactgtctc agaatgatta
     4621 ggtagataat gtctcattgt gatattatgt ataaagtatt catagcagga ggtctccgat
     4681 gaagatcacg cataataaaa ttggacagaa tttaaatatg gttgatggtg caaaaacgga
     4741 tcgcaccaaa accaatacat ctcctgcaat cggagctgag aaacttgatg gtttagaaga
     4801 attaggggca tcgaaagtcg aagtttctcc aagagctcaa caagccaagc gaattaaaga
     4861 gctggcgatg gcggctccgg atgttgatat ggcgaaggtc gcgaagtttc gtcaattgat
     4921 cgatgaagga aaatacaaag tcgacgcaaa agcggtggct gacagacttg tcgatgaaca
     4981 actggcaatg gacgacctta aaaattaata aggggatacc aaccctcttt atcaaggaag
     5041 agataggggc atatggaatc aacaattatt gaatcaacga cggatcgcag ttatcaaaga
     5101 ttggttgcca atcttgaaga gatgaccaaa ctttatcgtc aacttttgga tttggtacgc
     5161 aaagagaagc aacttcttgt tgatgccaat atggaaaagc tgcaagagaa taacgaaatc
     5221 aaagaaaatc ttttgtttaa aatcaaatcc gttgacggtt tgagaatgcg ttacgcggct
     5281 gagctcgcca ctctggttga agccgatcat gaaaatccac gtttactgga tattgcaaaa
     5341 aatctcggcg gtgtggaagg tgatcgtttg cgagtgatcc attcagcgct ggatcttcta
     5401 gttaagagac tttctttttt gaatagagaa aatgaagatg tcgctcaaac agcccttaaa
     5461 actcttaacg gagctttggg taatatcaaa gatactttaa gtggcaaaaa gacctatgga
     5521 gcaaaggggc aagtgcaacg aggccctgag ctggctggac actttgtaag caaagaaggt
     5581 tagaagctct ggatcgggac cgagcaggat gctcagttca agcaaagagg gacaagatgt
     5641 ctaagatttc tgccatgtta gatttgggca aacgttcttt gatgaataat cagacggctc
     5701 tccaaacagt gggtcataac attgctaata agtcgaccga gggcttttcg cgccagcgcg
     5761 tggaggtcgt gaccaatcaa ccacaaggtg aaggcaacct tcgtatcggc atgggggcac
     5821 gtgcgggagt tatcactcgg gtgaacaatc cttggttgga aaaacaaatc cagcgcgaag
     5881 gaagtaattt aggttttgcc gatgcaaaag cagacggcct aaatcgcgta gagcaagttt
     5941 ataacgagca aaccaataaa ggccttaatc aatatatgac gggttttttt aactcgtttc
     6001 gtgaactttc aaacaaccct gaatcccttt cttcccgaac catggtccgg gaggccagtg
     6061 aatccatgac taaagaattt ggtcgcgtga ccacgcaact tggtgatgtg cagaaagatt
     6121 tggacggaca actgatgacc cttactgagg aagtcaactt gatgtccaaa gaggtggcag
     6181 acctcaatga aaaaattcag attgttgaag ctcagggaat gcccgcaaat gatgagcgcg
     6241 atcgccggga tttattactt aaaaaaatgg gtgaaaaaat tgatatttct tgggccgaag
     6301 gaaaagatgg tcatatcagc gtgaccgcgg gccgaacggc tattttagtt tcaggaacaa
     6361 gttccactaa aataagagcg gcgcctacaa gtgataggaa tcgggttgaa attttttatc
     6421 aaagctcacc cacaggaact ccggtgaata ttaccaacca aattaaggga ggtcgtatgg
     6481 gcggagcttt ggaagtaaga gatgaagtta ttgaagggtt tctagactct atcgataaga
     6541 tggcttattc tctcgcggag gaagtgaata aagtccatat cgaaggtttt gaccgcagtg
     6601 gtaagccagg cgttttattc tttgagatgc cagagcaagt aaaaggagcc gcttccacac
     6661 tatcgctgaa ccgcaccatt gccaatgatg tcgggcgcat cgctgccggt tccgaatccg
     6721 gtgcgccagg ggacaataca gtagccaatg tgatttcctc tttgcagcat cgtcaagtca
     6781 tggatgacgg gaccgcgacc tttgatgatt tctacaatac acaagtgggt caggttggag
     6841 ccgtcactca aagagcaatc aagggacagg agtcccaaaa gaatgtggta gatcaattgc
     6901 aaaacattcg cgaaagcatc agtggtgtca gtttggatga agaaacgaca aaaatgattg
     6961 agtttcaaaa agcttattcc gcttctgctc gtttgattcg cgtggctgat gaaatgtttg
     7021 acaccgtact cagtttgaaa agaatgtgat tagaggataa agcccatgag gattgcagat
     7081 aaaatgcagt acaatcaagt aaatcagaat gtgacgaaaa atcgtacaga gatgtctgag
     7141 ttacagaatc aggcggccac ccaaaagcgt ctaacgaaac cttctgatga tcctttggcc
     7201 tctgctcgcg tcctttcggc tcgtacagaa gagcgtggca atcaacagta cattaaaaat
     7261 ataaataatg tgaaatcctt cttggaattc acggatcagt ctttgggaga gctcagtgaa
     7321 attcttatgc gggcgaaaga gttggcgatt ggccaagcca acgatgccag cggcaatgaa
     7381 caatctcgtc aagtctcggc ttcagaaata gagcaaatat ataatcaatc cgttcagatt
     7441 ggaaatcgaa aactcggcga ccgatttatt tttggaggct ctcagactca aacggccccc
     7501 tttgaccaac agggccagta tgccggcaat gatagcgaca tgaaaattgg catacacaag
     7561 gacacctttg tctcgatgaa tatccccgga aacaaggttt ttctcggaaa aggtttgagt
     7621 ggagatggca ttgttcgctc tcgctctgag accccaacga gtgtgaagtc tctgcaagac
     7681 ttcaagcagg aagagatcca acgtagggaa gagaatcaag atagagaagc caatttcgtg
     7741 gaaacccgcg ctcccgcaag cagttatcgc gcgcagagag tgggagcaat cgccaaggac
     7801 ccagtgacga atagtggggg cgtgaatcta tttcagacgc tgaaggggtt ggagatttct
     7861 ttaagagcca atgataagca gggaattcaa gaaaccctcg atacattaga tcaagtgatc
     7921 tctcaggtgg ttcaagcccg ttctgaggtc ggcgccagag ttatggccgt gaccaacacg
     7981 accgagtcgc tgcaaaaggc tattgtggat aataaagtga tggcttccca attggaagat
     8041 gtggaattat tcgacgtggt ttctagcatc aacaagacgg acagtactct ccgcgcaact
     8101 cttgaaacct cgggcaagct cattcagcaa agtctcatgg atttcttaaa ataagagtta
     8161 tcattaaagc gtcttgctcc aaaagaacac tcaggaattt agggcaattt attggaaatt
     8221 gcgacttaag caagcctcta attaatattt tatttgccta aatattagaa actatcccat
     8281 aatttaagag agagcaggga gtccataatg gatcgcagga aaatattaaa atttgctggt
     8341 ctggctagtg ctgctagaat tttcttcgga aaactgaatc ctgcatcagc tcaaacctgg
     8401 caaaaaatag ccaaccccac tggctcagcc ggaacctggc aaaaactcac catagcttcc
     8461 agtgtaaatg cgtacagtat ttcctcaatt gcgactgggg gacttacaca tacaggggtt
     8521 agcagatcag atggcttggt cttcactagt ggagcaaata attatggcca ggttggaaat
     8581 aacacagtag ttgctaaatc cacctttacc caagctctgg ggatttctaa tatagccatg
     8641 atcggtgcgg gacaacatca caccatagct ttgagatccg atggtttggt tttcggctgt
     8701 ggctacaata ctgctggcca agttggagat aatactgccg tcaataaatc cacttttgtt
     8761 caagccctgg ggatttcaaa tgcagtggcg gtcgctgggg gttattggca tacggttgct
     8821 ctgcgctcgg atggtttggt gttcgccacc ggagccgatt ggagtgggat tcttggtgat
     8881 aatgataata ctgggactaa taagtccact tttgttcaag ccctggggat ttcgaatgca
     8941 gtggccatct cctgtgggat aggcacctcg gtggccttga gggcggatgg gttggtcttt
     9001 gcctgtggtt ataatgatga agggcagctg ggcattaata cggtcgcgag taagttaacc
     9061 tacgtccagg ccctggggat ttcggacgca gtggccatcg ccgccggaga acgatttgta
     9121 gtcgccttaa gatccgatgg tttggttttc ggctgcgggc taaacggtca cggtcagctc
     9181 ggtattaatg caacaacgaa tcgatccacc tttgtacaag ccataggcat ttcaaacata
     9241 acagcgattg ccggtggctc ttttcatacg ctcgccttga gatccgatgg tttggttttc
     9301 gcctgtggct acaatgctgc tggccaagtt ggagacaata ctacagctaa taagtctact
     9361 ttcgtgcagg ccttaggtat ctcaaacgcg atagcaatcg cttgtgaagg aaatagttca
     9421 gttgccttga gatcagatgg gttgattttc gccactggaa ataattcaaa tggccagctt
     9481 gggaataatg ccacagttag taagttaact tttgtaagcg cgatcgcccc ctgagtgtga
     9541 ggcttttcct ctacagaggt cgggttagag tgtggcatta aacaagactg atagcgccat
     9601 ccagacaatc ttattcaacc aagcctcctt gactttctaa aatagaaatt atacctacag
     9661 ttccgcttaa acgtgccgat aaataatcgt gcatagtatc tgaacgtgta aggatgcgta
     9721 taagggtaag aagcccctgc taggaataaa ggagtaatcc tatgttggtt ctcacaagga
     9781 agttgggtga aagcatcgcg attgatgatc atatcaaaat tcgtgttgtt caaattaaag
     9841 gtaaacaggt ccgtttaggc atagaggcgc ccaaggatac taaaattcat cgtgaagaag
     9901 tctacttggc gattcaagag caaaaccagc aatctgcgac tgtttcttcc gataaaactc
     9961 gttctgttgc aaaactgtta aaaccttaaa gtctttgaga gccgcatggc tcttacaaat
    10021 ccagacaagc ccttttagaa gcgctctgtc atgatgcaca gatttcattt gagtcttccc
    10081 taatttgggt ccagatttat aataaagtta accttttttt atggattcta tgtctgtaag
    10141 gaacgggttg atttgtcaat acttgcaggg gggctgcgtg attatcaata cttccagatt
    10201 tggccgagta gaattgcaaa acaatgatgt tttgactttt cctgaaggcc ttttgggttt
    10261 tgctgatctc cgccgctttg ttcttcttga tgatccaagc gatgagattt ttgcttggct
    10321 tcaaagttgt gagtcttccc atattgcttt tccagtgctt gaacctgaac tttttgtcga
    10381 tacttacaag gcgaccctta caaagggtga tcttgaggct ttaaagcttc agtcccagga
    10441 taatgctcgt tattttacta ttgtgacaat tcccgatgat ccaacacaaa tgactgccaa
    10501 cttaaaagcg cctattgtcg tgaacataag cgagaagatg gctcgacaat gtgttctcca
    10561 agataataac ttggcaattc gtgagcctat ttttactaag cttcagcagc gagtggtgca
    10621 gaatcctgct actcctatta agaaccagtc tgctggtatg gatgtagcta taaaactggg
    10681 caacaagaac agttctgatc agattcttta agttcttttg ttaataagca gtgacctgtt
    10741 cgagctctga tgttttaatc atgaacaact tgtgaatttt gttcgattta aaataaaagg
    10801 cattctagtt catgaccaaa cgaagatcga tatctgtaag ggagcctcgt aaagagccca
    10861 agcaagagcg ctctcgccaa acctaccaag caatattaca gggcgcggcg aaaataatcc
    10921 gcaaaagcgg agttcaaaag tttagcacca ataaagtggc taaagaggct ggagtcagca
    10981 ttggctcttt ataccaatat tttccttcca aagaagcaat tctagcggcc ttgattgatc
    11041 aaattttggc atatgaatat agttatatga aagagcgttt tcggaatttt tcgccggaga
    11101 taggggctcg ggaagttttt aaacaagtat tttcttatta ttatataacc gagcccgaag
    11161 agttgaatta ccgaaaaatc atgatggatg cagttgcctg ggtgaataaa acggcgatag
    11221 ctttgaagtt ctataaatcc atcgctgagc ttattgttaa ttttacgcaa gagcactttg
    11281 atagcccaca aagcacgaga gataatgacg ctgcggtctt tgtgattcaa tacatgctgc
    11341 gtgcgacgat cttctcctcg gtggataaag aactggagaa gatcaatctt gagaaattga
    11401 tagacgaact cgccgaaatc ttaatgggca ttttaagaat cccaactgag catcgtggcg
    11461 ctctccctga agagaagtag agctttattt aaaattatcc ctcaatctgc ggtaaggatt
    11521 tctcagtttt agcgcccatg gattttaaat cttccacttg gcggatcaga tttcctttgc
    11581 cagtcataag cttacccatg acttcatcgt gagctttctg ggcggcgccg agtttatcac
    11641 caaggttttc gatgtctttg acgagaccca cgaatttatc atagagcgcg cctccgcgct
    11701 ttgcgatatc cagggcattt ttttgttggc gatcttgttt ccatagggaa gaaacagtcc
    11761 ttaaagttgt caatagagtc gtgggactga cgatggctat atgtttatcc catgaactct
    11821 ggaaaagatc tggttgtaat ttaaaagcca aagcaaatgc aggctccagt ggcataaata
    11881 aaattacaaa gtctgggctg accagtttat caatggcgta atattttttt tcacttaagc
    11941 cattgatatg ttttttaagt gcttcgacat gaagtttgcc gagacgttct tgatcctcaa
    12001 gcgacgttgc cgaggagtaa gcctcataag cattgagtgt catttttgaa tcgacgatca
    12061 agtgtttgcc ttcggggagt ttgacaatca catctggacg aaggacttgg ttgtcttcac
    12121 cacgaagatc catattcgtc ccttgaagaa tatactcttc gcccttacga agccccgaac
    12181 gttccagaat attttcgagg atgagctcgc cccagtttcc ttgcgtttta gcttctccct
    12241 tgagcgcttt ggttaaattg acggtttcgt tcgacatgag acgatttaat tccataagtt
    12301 tgcccaactc accgcgaaga gctcctcgtt cagcgcgctc atgcccatag gtctcttcca
    12361 ctttcttttc aaagtcttta agacgctcct tcagggggtc caggacggta ttcagatttt
    12421 gcatattctg agtggtgaac ttcgtgctct tttcttcgaa aattttatgg gccatatttt
    12481 caaattgctg agacatcttt aattgcagat cagcttgttg gcctcgcatt tgctctaagc
    12541 ttcgggcagt aaactcattt tgcaattgaa agctctgttg cttgatgcgg taaagaaacc
    12601 aagaccccac agcggtcata agggctccag ttaaaaaggc caatatcaaa gacatcatag
    12661 attatgctcc ccaagttatc tatccagtga tttaatgatg gtttttcatt tggtcactga
    12721 aaataaattc tgctattaat attagcatca tactagaggc tctaatgcaa agaatcaaaa
    12781 attggcttat agcgttccgg ccaaaaaccc tcacggcctg tgtagtaccg atcctagtcg
    12841 cgagtgcctt ggtgaaagct ttgcatttgt ccgtggatgt cgaggtcgta gttttggctt
    12901 tattcgcgag cttttttatt cagattggaa ccaatctggt caacgatgcg gttgatttta
    12961 aaaagggagc ggatacggaa aaacgcatgg gccctcgaag agtcactcag tccgggctgt
    13021 tctcttataa acaggtgatg ctagcggcgg ggctttgttt tatatttgcc gtggcttgcg
    13081 gaattccctt agttatcaag gggggcactg cgattctagt gatcggggtt ttatccgtac
    13141 tcatgggcta cgcttatact atggggccga ttcctttggc ttatttcggc ttgggtgatc
    13201 tctttgtcat tctttttttt gg
//
LOCUS       DATBTE010000429         4709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_672865, whole genome shotgun sequence.
ACCESSION   DATBTE010000429 DATBTE010000000
VERSION     DATBTE010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672865"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VIG33_16385"
     CDS             complement(<1..889)
                     /locus_tag="VIG33_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272472.1"
                     /translation="MIPFFILFLGMSSLFACLGALAVGHLVPAVSASALLLALATAWF
                     LARDFKLVLNPPPIGVPVFLMYALIFVGIYFHSIFLFFPKSESYWIQDPFNLGDMSFH
                     WHTIRFLGKGATFWPENPIYMGYRFKYPFGMDFFNALFENLGVAISSHLPVVTLLTLV
                     LVFYVLHMAGGPLLVFAIFFSSGMYNFTVPGNWDLNEMQNTVDFKNLFLTVLLTQRGF
                     GYALPAGVFLYRALQECFSGAWKPSFLEKLSLGMIWGGLGFFHLHSFFFVSMYLGILI
                     LWKRDLRNWLLPLGIASLLG"
     gene            complement(886..2436)
                     /locus_tag="VIG33_16390"
     CDS             complement(886..2436)
                     /locus_tag="VIG33_16390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY8272473.1"
                     /translation="MTKFFLKFKEKAFGNPYLCALSLVFLLVAALSRFLYLGNKPIHF
                     DESINMWFVKRIWEEGYFTYDPTNYHGPLLFYLIQFVQLFTGFDFLSTRWVATVFSFL
                     TLAALWFGPVAERRAFRWSAVFLLFSPAMGFYGRSGIHESAFVFFQVLGFLSFHFLVA
                     KEFKNFWWTFAASLLGMMALKETFVILILAVIPALIMVWFTDIRKTNLKKWFNDLSAS
                     LQQREVYFPLLVMLLLFIGVYSGFGAHPRGLIDFVVALMPWLKTGVHGNGHEKEFFHW
                     LKFMANNEYATFLVFFAGLPFVIKNKWLRFYFVFTFFLWLIYSLIPYKTPWCLISILW
                     PFALVAGFGLAEWTRKISGWKEALFYAALLILFGGEFSLMYELMYRNPINMDHPYVYV
                     NSTYQMKEFIEKTQALLKEDPLLREQIVQIGTEESWPMPIVFHNFYTLSYFKANARVE
                     PEALIYMVDEKDQASMEAQLKERNHSALYQVFSLRIRQSRSSVLVYLKKKYFENKFSW
                     QLKEVGEL"
     gene            complement(2559..3554)
                     /locus_tag="VIG33_16395"
     CDS             complement(2559..3554)
                     /locus_tag="VIG33_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272474.1"
                     /translation="MRRLFLIYFLFFGIFADASGNGNQQINLIISVDWEGFSLEDANL
                     EAFRKFREDYPEVKIVHFLNAAYFLQKGADAPSIQKKINSVIRPGDELGLHIHAFETL
                     LEAAGVKYREDYNFWGREKSKPINNVRGHDVPLSIYSESEIRKIVRTSLNILEENGFK
                     NLKSFRAGGWAASPEVLNALVKEGLTIDSSAVPPEIVKNVTGEETPLYSKIVNKLWQQ
                     TTVQSDTAYEIKTETGTIVEVPNNFGLADYITGEEVFEKFKVLFDNLDFNSNRPVNIH
                     YGFHEETAAQFIGRVRYAIDKIKEHVQAYRVDINPKSLRELKLTSGSALSCERLF"
     gene            complement(3723..4193)
                     /gene="lysM"
                     /locus_tag="VIG33_16400"
     CDS             complement(3723..4193)
                     /gene="lysM"
                     /locus_tag="VIG33_16400"
                     /inference="COORDINATES: protein motif:HMM:NF008399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HEY8272475.1"
                     /translation="MGLINFLKNAGEKLLGHKEADVESIKKYIQSQGLSTEGIDISFD
                     PAKDIVTVSGEVPDQATKEKILLCCGNVQGVASVQDNLRVRKPAPESQFYTVQSGDTL
                     SKIAARFYGDAKKYSQIFDANRPMLSDPDKIYPGQNLRIPSQAPAQEKVAEHHP"
BASE COUNT         1512 a         1013 c          957 g         1227 t
ORIGIN      
        1 atcctaaaag ggaagcgatg cctaggggta aaagccaatt ccgcaggtcc cttttccata
       61 aaatcaatat tcccagatac atactgacga aaaaaaaact atgcaggtga aagaatccca
      121 atccgcccca aatcattccc aaactgagct tttccaaaaa tgagggtttc caagctccag
      181 aaaaacactc ctgcaaagcg cgatataaaa acactcctgc gggaagtgca tagccaaagc
      241 cccgctgagt taaaagcacc gttaaaaaca gatttttaaa atcaacagtg ttctgcattt
      301 cgttcaagtc ccagttgcca ggcactgtaa agttatacat cccactacta aagaaaattg
      361 caaaaaccaa aagaggtccg ccagccatgt gtaaaacata aaacaccagc accagtgtca
      421 gtaaagtgac cacgggcaag tgactactga tagccactcc tagattttca aaaagcgcat
      481 taaagaaatc catgccgaag ggatatttga aacgataccc catgtagatg ggattctcag
      541 gccaaaatgt ggcccctttt cccagaaaac gaatggtgtg ccaatgaaag gacatatccc
      601 ccaaattaaa aggatcttga atccaataag attcggactt cggaaaaaat aagaaaatcg
      661 aatgaaaata aatccccaca aagattagag catacataag aaaaacaggc acgcctatcg
      721 gtggggggtt caatacaagc ttaaaatccc gagctaaaaa ccaggccgtt gccaaagcca
      781 acaataaagc gcttgcgctg actgccggaa ccaaatggcc tacagccaag gcccctagac
      841 aggcgaataa agaactcatt cccagaaaaa gtatgaaaaa tggaatcaca actcacccac
      901 ttcttttaat tgccacgaaa atttattttc gaaatacttt ttcttcaaat aaaccaaaac
      961 agaagagcga ctttgcctga tcctcaaaga aaaaacctga taaagagcag agtgattgcg
     1021 ttctttgagt tgggcttcca tacttgcttg gtctttctcg tcaaccatat agattaaggc
     1081 ctccggttca acacgagcat tggctttaaa ataacttaaa gtataaaaat tatgaaagac
     1141 aatgggcatc ggccaagatt cctcggtccc aatctgtaca atttgttctc ggagaagggg
     1201 atcttctttg agcaacgcct gagtcttttc aataaattct ttcatttgat acgtcgaatt
     1261 cacgtagaca taaggatggt ccatgttgat cggattccga tacatgagtt catacataag
     1321 ggagaattcc ccaccgaata ggatcaataa ggccgcataa aaaagagcct ctttccagcc
     1381 agaaattttt cgtgtccatt ctgccagacc gaatcccgcc accagagcga aaggccaaag
     1441 aatcgagatc aggcaccagg gagttttgta aggaatcaat gagtaaatca gccacaagaa
     1501 aaaggtaaaa acgaaataga agcgaagcca tttatttttt ataacaaaag ggaggcccgc
     1561 aaaaaaaacc agaaaagtgg catactcatt attagccatg aacttgagcc aatgaaaaaa
     1621 ttctttttca tgtccattgc catgaactcc ggtttttaac catggcatca gggctacaac
     1681 aaaatcaatc agtccccggg gatgagcacc aaatcctgaa tagactccga taaacagcaa
     1741 aagcatgacc agcaaaggaa aataaacttc acgttgctgc aaacttgccg ataaatcatt
     1801 aaaccatttt ttgaggtttg ttttccgtat atccgtgaac cataccatga tcagtgccgg
     1861 aatcaccgct aagataagaa tcacaaaggt ttctttcaaa gccatcattc ccaggagtga
     1921 agccgcgaaa gtccaccaaa aatttttaaa ctccttggct accaggaagt gaaatgatag
     1981 aaatcctaaa acttgaaaga agacaaaagc ggattcgtgg ataccagagc gtccataaaa
     2041 tcccatcgca ggactaaaca gcaaaaatac ggcagaccaa cgaaaggccc ttcgttctgc
     2101 cacgggacca aaccacaaag ccgccaaggt cagaaacgaa aaaaccgtgg ccacccaacg
     2161 agtgcttaaa aagtcaaagc ccgtaaaaag ctgaacaaat tgaatcaaat agaacaacag
     2221 aggaccatga taattggtag gatcataggt aaagtagccc tcttcccaga ttcttttaac
     2281 aaaccacata ttgatgcttt catcgaaatg aatgggctta ttaccaagat agagaaagcg
     2341 agagagcgcc gccaccaaca aaaagacaag gctcagagcg cataaatagg gattaccaaa
     2401 ggctttttct ttgaacttta ggaaaaattt tgtcatggaa tcattctagg attcgaaagc
     2461 cctaaggaaa agtgaaaagt gactccacag acttaggact ttaatctagc gcaatgagac
     2521 tttctgccgt ggtcgtaaac ttctttttct taaatttttt aaaatagtct ctcacaggat
     2581 aatgcggagc ctgaagtgag ctttaactcg cgaagacttt ttgggttaat atcaacacga
     2641 taggcttgaa catgctcctt gatcttatct atggcgtagc gaacgcgtcc aataaattga
     2701 gccgctgtct cctcgtgaaa accatagtgg atattcacgg gtcggtttga attaaaatca
     2761 agattatcaa ataacacctt aaacttttca aaaacttctt ctccggtaat ataatccgcc
     2821 agaccaaaat tattcggtac ttcgacaatg gtcccggttt ctgttttaat ttcgtaagca
     2881 gtatcacttt gaacagtggt ctgctgccat aatttgttta caattttgct gtaaagaggg
     2941 gtttcctcgc cggttacgtt tttcacgatt tcgggaggta ctgctgaact gtctattgta
     3001 agaccttctt ttactaaagc attcagaact tcagggcttg cagcccaccc ccccgctcga
     3061 aacgatttca aatttttaaa accattttct tccaatatat tgagactggt tcgtacgatc
     3121 ttccgaattt cgctttcact ataaatagag agaggcacgt catgtcctct gacgttgttg
     3181 atcggcttgg atttttcccg gccccaaaaa ttataatcct cacgatattt cactccggcg
     3241 gcctccagaa gagtctcaaa agcatgaata tgcaagccaa gttcgtcacc gggacgaatc
     3301 acagaattta ttttcttttg tatactaggg gcatcggccc ctttttgcag aaaataggcg
     3361 gcatttaaaa aatggacaat ctttacctcg ggataatctt ctcgaaactt cctaaaagcc
     3421 tctaggttgg catcttctag actaaagccc tcccaatcca cggaaatgat gagattaatc
     3481 tgttgatttc catttccgga agcatcagca aaaataccaa aaaataagaa ataaattaaa
     3541 aagagtcgtc gcatgaaatc tctttatgcg acgacagtgc caacctcaaa gctcaattat
     3601 tcccctttga aggcggagcc acgtctaaga gttttacaaa gtgccgtgaa gcttcgtcga
     3661 actttttgac atgcattttt gacatgcatt tttggcattg tggtttcctg aactattaaa
     3721 gactatggat gatgttcggc gactttctct tgtgcgggag cttgagaggg aatgcgcaag
     3781 ttttgaccag ggtaaatctt gtccgggtct gaaagcatcg gccgattggc atcgaagatc
     3841 tgtgaatatt ttttggcatc gccataaaag cgggcggcaa ttttagacag agtatccccg
     3901 ctttgcacag tatagaactg ggattctggc gcgggtttgc gaactcgtaa gttgtcttga
     3961 acgctggcaa caccctggac attcccgcaa cagaggagaa tcttctcttt agtggcttga
     4021 tcaggaacct cgccagaaac ggtgacgatg tccttggctg gatcaaagct gatatcaatc
     4081 ccctcagtag agagtccttg ggattgaata tattttttaa tcgactccac gtcagcttcc
     4141 ttatgaccca agagtttttc accagcgttt ttaagaaaat tgataagtcc catggcggca
     4201 catccttcag aaatgttttc aaaatactag cagaacctgt ggatgttgct tggtggtttg
     4261 tatcttttta aattcaaaaa atgaaaatgc tatttgcttt atttcatttc gcacagataa
     4321 aagggcagtt tgcttcgcaa agtggcccca ttgacgacgt taggggaagg gaggaggaga
     4381 agaggggttc tatgagtcag gatcgaggtc cgaattccga acctgagaag gagtaaagga
     4441 ggttagaggg gtttttcttt tactgatgag tttcctgttg acgagggttt tgaatctcta
     4501 aaacttgcta gaactcgctt ttttatcact caattaaaaa tgtttagagg agcctgcaat
     4561 ctgctagggg cgaagctttt atgctgttta agcctctggg aaaagtcatg gtataacttt
     4621 ttttctcgag aggttttcag gtgcgacaaa ttcattttga atttttaaaa aactataaac
     4681 ttaaatttgg cggtgagttg ctcaaaggg
//
LOCUS       DATBTE010000430         2917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1220080, whole genome shotgun sequence.
ACCESSION   DATBTE010000430 DATBTE010000000
VERSION     DATBTE010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220080"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VIG33_16405"
     CDS             complement(<1..453)
                     /locus_tag="VIG33_16405"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8272476.1"
                     /translation="MFNYNHLYYFYVTARLGGVSNASRYLTISQPSLSVQIKTFEAST
                     NRKLFKKNGRKMQLTSEGEQVYAYCQQIFEIADYLSEHLKDPGLKEKRRIHIGVSDQI
                     ERPFMGDLLGDILQQGKEHLNCILNVSSGEEQELTEKLRDQSMDLVLTN"
     gene            545..922
                     /locus_tag="VIG33_16410"
     CDS             545..922
                     /locus_tag="VIG33_16410"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEY8272477.1"
                     /translation="MQVQTCFRDLDKTDTLEAYLRDKIGSSIENFLKKDPNSTATVRI
                     ELDRHRNQNRKPSFVCEVILKPTHQKGTLKVSKGGEDFYTAVNEAAAALRTILRRRST
                     RKSQHRRHEHTREIRDLFEEWVA"
     gene            1057..1620
                     /locus_tag="VIG33_16415"
     CDS             1057..1620
                     /locus_tag="VIG33_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY8272478.1"
                     /translation="MHILILNGSVHGDRGNCGIIIQEIRKRHQQINWSLVNLSKTKFG
                     PGLEKKIKKSDAILCLTGTYWDSWGSPLQKFLEEATDLEARPEILGKPAGVCVLMHSV
                     GGKSVLSRLQGVFSSMGFLIPPMSGMAYSLSGQLALKSKSMNAKDFWCLEDIEVIVQN
                     LVIAVEKKILWKTWPIDRKNFRKTWVK"
     gene            complement(1673..2293)
                     /locus_tag="VIG33_16420"
     CDS             complement(1673..2293)
                     /locus_tag="VIG33_16420"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEY8272479.1"
                     /translation="MFKLPTLPYAKTALTPFLSEEQMTYHYDKHHKAYIDNLNKFIET
                     DASLKGKSLEEIVLSSTGGIFNNAAQAYNHTFFWYNMQPASTMKPSSALMAAISRDFG
                     NLDQLKEKFVEGGVKTFGSGWIWLCADNAGKLSLVSTSNAQVPFTNNGPRPLLVADVW
                     EHAYYIDYRNLRAKFLETFWGNINWDFVSQNFESNKVREVTTSMKA"
     gene            2468..>2917
                     /locus_tag="VIG33_16425"
     CDS             2468..>2917
                     /locus_tag="VIG33_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272480.1"
                     /translation="MVVQALMYKETEDFLIKQIVFDLAKPGFELAFVSHRTNLYYAYF
                     PKNAVAPSSAVVKLLQGLFDLQRDRSFFILRNRIYTTAPLSAMCQGMIKIVAKRALGN
                     VQAQNHHFPIPYEKIMVGGGGTLAPVSFLNTENQLPLEQIKNLEEASK"
BASE COUNT          882 a          572 c          621 g          842 t
ORIGIN      
        1 gttcgtaaga accagatcca ttgactgatc ccgcaatttt tcagttaatt cttgttcttc
       61 gccggaactg acattcaaaa tacaatttaa atgctctttt ccttgttgaa gaatatcccc
      121 taataaatca cccataaaag gtctttcaat ttgatcgctg actccaatat gaatccgccg
      181 cttctccttg agaccgggat cttttaagtg ctcggataaa taatctgcaa tttcgaaaat
      241 ctgctgacaa taggcgtaga cttgttctcc ttcgctcgtt agttgcattt ttcgtccatt
      301 ttttttgaaa agcttacgat tcgtggatgc ttcaaaggtt ttaatttgaa cgctcaatga
      361 aggttgactg atagtaagat atctagaggc gttgctaact cctccgagac gggcggtcac
      421 ataaaaataa tagagatgat tgtaattaaa catataaaaa tcctaagtat aggttagtaa
      481 atatatattt tatctatcat cgtaaatcaa cgatacttgt cgtagaaaca agaaggagat
      541 cgttatgcaa gtacagacat gcttccgtga ccttgataaa actgacaccc tagaagccta
      601 cttaagggac aaaatagggt ctagcatcga aaacttcctt aaaaaggacc caaacagtac
      661 cgccacagtt cgtatagaac tggaccgaca tagaaaccaa aaccgtaagc cttctttcgt
      721 atgcgaagtg attttaaagc ccactcatca aaaaggcact ttgaaagtat cgaaaggcgg
      781 agaggatttt tacacagctg tgaatgaggc cgcagctgcc ttacggacta ttttacgacg
      841 acgatcgact cgcaaatcac agcatcgacg ccatgaacat acaagagaga ttcgtgattt
      901 attcgaagaa tgggtggcat agagctattt taggacaaat catggtttaa aattcttttc
      961 cctttaaaag taggagccct ccgggctcct tttttattgc ctgcttcttt ggagcacttt
     1021 tatgatgact gcctggcata cttaatgagg atttaaatgc atatacttat cttaaacggc
     1081 agtgttcacg gggacagagg aaattgcgga attattattc aagaaatacg taaaaggcac
     1141 cagcagatca actggagctt ggtaaatctg tcgaaaacaa aatttggccc aggtttggag
     1201 aaaaaaataa aaaagtcaga tgctattctt tgtctgacgg gaacttattg ggattcttgg
     1261 gggagccctc ttcaaaagtt tttagaggaa gcgacagatt tagaagctcg acctgaaatt
     1321 cttggaaagc cggctggagt ctgtgtactt atgcattctg tgggtgggaa atcagtgctg
     1381 tcgcgcctgc aaggagtttt ttcaagtatg ggtttcttga tcccgccgat gagcggaatg
     1441 gcctattctt tgtccggtca gctggcgctg aaatcaaaga gtatgaatgc aaaagatttt
     1501 tggtgtcttg aagatatcga agtcattgtg cagaaccttg tcattgcggt cgaaaaaaaa
     1561 atcctgtgga agacgtggcc cattgatcga aagaatttta gaaaaacttg ggtgaaataa
     1621 agagtcagaa aaagaaaccc ttacctggtt tggtaagggt ttcaaaagat tcctaggctt
     1681 tcatagaagt ggttacttca cgaactttat tagattcaaa gttttgagaa acaaaatccc
     1741 agttgatatt tccccagaaa gtttcaagga atttagctct taagtttcta taatcgatat
     1801 aatacgcatg ttcccatacg tcagctacga ggagcgggcg aggtccatta ttagtaaatg
     1861 gaacctgtgc attagaagtg gaaacaaggc tcagctttcc agcattatca gcgcacaacc
     1921 agatccaacc agaaccgaaa gttttcacgc cgccctcaac gaatttttct ttgagttgat
     1981 caaggttgcc aaaatcacga ctgattgccg ccatcaaggc agagcttggt ttcatcgtag
     2041 aggcgggttg catattgtac caaaagaaag tgtggttgta agcttgagcg gcattattaa
     2101 aaataccacc agtggatgaa aggacgattt cctctagaga tttgcctttt aatgaagcat
     2161 ccgtttcgat gaatttattt aaattatcga tataggcctt atgatgttta tcatagtgat
     2221 aagtcatttg ctcttcagat aaaaaaggtg tcaaggcagt tttcgcgtag ggaagtgtag
     2281 gaagtttaaa cataggtgtc tccttagatt gaactgagtt ttaaattttt ctcaattgat
     2341 ttccaaggac acactataac caggaagatc ccacaaggtc caatttaaat gctccctgcc
     2401 aatatatttc cgattgtgct ttaacacctc taagcaaaag tgtaagagag acaatagggg
     2461 ccatcttgtg gtagtccaag ctctcatgta caaagaaact gaagatttcc tcattaaaca
     2521 gatcgtcttt gatcttgcga agccaggctt tgaacttgcc ttcgtaagtc atcgcacgaa
     2581 cttatactat gcttattttc caaaaaatgc ggtcgctcct tcttcggcgg ttgtaaagct
     2641 tcttcaaggc ctttttgacc ttcaaaggga tcgttctttt tttattttaa gaaatcggat
     2701 ttacaccacc gccccactct cagcgatgtg tcagggcatg attaaaatcg tggccaagag
     2761 agctctaggc aatgtgcaag ctcaaaatca tcactttcct atcccttatg aaaaaatcat
     2821 ggtcgggggg ggaggaacgc tggcaccggt gagttttttg aacactgaga accaattgcc
     2881 cctggagcaa atcaaaaatc ttgaggaggc ctccaag
//
LOCUS       DATBTE010000431        11292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_332417, whole genome shotgun sequence.
ACCESSION   DATBTE010000431 DATBTE010000000
VERSION     DATBTE010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 11292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11292
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332417"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VIG33_16430"
     CDS             <1..327
                     /locus_tag="VIG33_16430"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HEY8272481.1"
                     /translation="ICLDPFYSVAADLTIHRPGEVIFIPRLVDIQLPDGSKHNGYLII
                     RDEGGAIIGENRFDFFTGFFGPYDHGNVFSELGLGDKKNRFGYQKVSAEVAKMVREYR
                     NYPNIP"
     gene            complement(337..618)
                     /locus_tag="VIG33_16435"
     CDS             complement(337..618)
                     /locus_tag="VIG33_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8272482.1"
                     /translation="MKENKSLADFLKNKRIESGFSQLDIAKKLGYTSPQFISNWERGL
                     SNPPIYTLRKLTEIYQIPPDTLFEIALKTIISQITLDMRKKFYGKKGKR"
     gene            804..2069
                     /locus_tag="VIG33_16440"
     CDS             804..2069
                     /locus_tag="VIG33_16440"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEY8272483.1"
                     /translation="MKTLLSMSIMFGLFTLAHAKTSINSMCYRLDQPGSNMQGEHVGE
                     LFEIASVSKVVTSYWAIRALDPNFKFSTHIHITPVGSHFFDVHIQGSHDPFWGREMTH
                     FLFAQLNKIGVHEIRKLTFDENLIFRWSVISNYIDPINPSFQEIAEAFHNHLKNLPKE
                     YPKTHSEAALLGLYLPKMLSLKAQSVAYLSSFDFKEESQTVTYALQSVPLYRYLKEMN
                     SVSNNHVADHLFNYLGGPEKFKDFIQQDMNLNSKDIQFINGSGNSLTGINENGESVKE
                     YNKASCDTMLRILFKVRAQLKKFGLDLKDVMAVAGTDEGTLKPRYDSLRNSLVAKTGT
                     VDPAVTLAGVISTTQGNVYFGIFMETESSADWSNARDDIRQKVIELMAQFGGRRSISY
                     RTRGFMPFDQDSGMIREIRQSQTQIKPKP"
     gene            2239..3063
                     /locus_tag="VIG33_16445"
     CDS             2239..3063
                     /locus_tag="VIG33_16445"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY8272484.1"
                     /translation="MLRVSGMSASLMAQRYLGTTQRSTEKAMKDLASGTRFSTAGTDA
                     AGAAIAQQLEGQARGQKAALNNADNATSFIQVAEGALNEQNNILIRLRELAVQAASDT
                     YSDKEREYINSEYTQLSQELDRIAKTTTFGATSLLNGRSKNYEFQVGTSSGEDSIIRY
                     TSDTNTTASELGISGGSVESKSEARNDLKDLDEAMAKIASARAKYGAIQSRLDSAANS
                     LAIGVENITAAHSRMADTDVAQAVSDVRRGQILQQYQASVLAQANSSNEILLRLLA"
     gene            3442..4266
                     /locus_tag="VIG33_16450"
     CDS             3442..4266
                     /locus_tag="VIG33_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY8272485.1"
                     /translation="MGLRIQTNMASISAQRVLSVQQKRTEHSAAALASGSRIVHAADD
                     AAGLAISENIRGQTRGIAQARNNAFNAGSAIQVSEGGLNEISNILIRLRELGVQAASD
                     HIGDRERAFLNLEATQLTEEADRIARTTVFGDKKLLDGSGGKMEFQVGAFGTKENVIS
                     YDLGSNATASELGIEGLSVESKGDARSALQTVDDALVKVSSLRANFGAVQSRLDSTVS
                     NLDVQYENLSAANSRIRDTDVAKESSEMMSANVLQQAAVSVLAQANQTSAVALKLL"
     gene            complement(4478..4945)
                     /locus_tag="VIG33_16455"
     CDS             complement(4478..4945)
                     /locus_tag="VIG33_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272486.1"
                     /translation="MKMLLQMLLSMLFVVTASGHDPIVPWPLDVNREYIESQDIVGTW
                     VALDRINNKLWYLKITKPPIRSNTSVTISLLGKGFSKPQMGWLVPVDGVYAGKITTSK
                     DQESPLMIFKNLDGLYLRLETEKEEYQDFILYRIYLSPRFKKTPKDLRLPGRA"
     gene            complement(4942..6066)
                     /locus_tag="VIG33_16460"
     CDS             complement(4942..6066)
                     /locus_tag="VIG33_16460"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8272487.1"
                     /translation="MTQNFLPSLKIARLQYQSGDFLQAKNSIEEACKEAHQLGDVSQW
                     LEALRIQIQCFIELEQLEKTEQQETELLEFLKKNTDLFHAATAEYILGILLLNHGHRS
                     QAQAYLASAISKASQTKNIRSLAYALYADSYLKTSSSDKDLPQASVNLEKIEVLSSQE
                     PLAELMISVRLLRCYIQTELGHPEQALLLLWEAYESAKLQGLNVVVSSILAQMARIHQ
                     NANRQSLYQIYADLALCGVDKDKHPRLFRQISQIYADATPRSHDIEVDEDKRLILEKK
                     RGAINFGNQHILMDMALLFLKNPGKRYSKSELFEYIWQQPYDSLTHDNLIYVSIKRLR
                     TLLEPDDSPQYLLKDRKGYYLNPQITIQFKSDNKCNKEIA"
     gene            6553..7386
                     /locus_tag="VIG33_16465"
     CDS             6553..7386
                     /locus_tag="VIG33_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY8272488.1"
                     /translation="MGIRISTNVASINAQRTMAGGQREIQKSMAQLASGSRITKSADD
                     AAGLAISENLKSQMRSYTQAQRNANDGISLVQTAEGGLNEVSNILTRMRELSIQASSD
                     TVGDRERGFTDKEVQQLKQEMQRIAQTTRFGNTKLLDGSGSEFDFQVGINNKDDEDRI
                     RYDASMTNAQIDNLGVDGFDFTSKEGAQSALEKIDSAQSQVNGYRASLGALQNRLQST
                     SDNLGVMNENISAANSRIRDTDIAAATSEMTRNSVLLNANTSVLGQANQLPQLALKLL
                     G"
     gene            7567..7944
                     /locus_tag="VIG33_16470"
     CDS             7567..7944
                     /locus_tag="VIG33_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272489.1"
                     /translation="MNIKGLLGNILPTNPIRLIQKTEKTIKSDSTSDRDANGQQAGGG
                     EQQQREPMTEEQLNKALEQVRNLSGVKEHNWSVELSVIENRRFVVVKDISGNIIRRIP
                     ELDLWTLPEGNESGKGQIFKKTA"
     gene            8196..8930
                     /locus_tag="VIG33_16475"
     CDS             8196..8930
                     /locus_tag="VIG33_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272490.1"
                     /translation="MSMIVVKRAPKLNNDLVHGLGLGLINRVDLYYESTASILNAIVA
                     LGTSHNEYAKEFALSLLQKSDPYYENTDTQLKSAVASLRNAANAPSGDSSEIWSFRSE
                     NKVCKTIPGQWGDYYPCPKYTASVSDQKGNRILLTCRSNEFGINFRIRPSRELWSQLA
                     NAKIEGLGFGTDKIHQWLGIPYLISEDFSLFAEEALSEELLNHLRSDKVLNITLRLNS
                     GESNRSVRFNLSGSNKAIEKLLSYCR"
     gene            complement(9018..9965)
                     /locus_tag="VIG33_16480"
     CDS             complement(9018..9965)
                     /locus_tag="VIG33_16480"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEY8272491.1"
                     /translation="MEELEDFEKKIQTLSDQELVAQTGSFFKREKRMGDIILLSLREI
                     KARRIYSKLGYSSLFEMLIGYYHLSEGPSYQRLNALKLIETVPEAQELIFKGEVSLSN
                     AALVQGLIQRTEKEAPLSLTEKQELLESVKGKTTKQAKASLTAQHPVASLPLNQEKPL
                     SATHTQILLTLDQETLKLFKEAKDLLSHSIPNGDLSEVLKSISIIAVEHLAKKKGRTP
                     PLKSPTSVKQSVDDSPIPSRYISQEIKRIVFARAGGCCEFVGSEGHRCTSRFQLEIDH
                     LYPWSQGGSNRAENLSLKCKSHNFYRTKETHGFFYNVKK"
     gene            10835..>11292
                     /locus_tag="VIG33_16485"
     CDS             10835..>11292
                     /locus_tag="VIG33_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HEY8272492.1"
                     /translation="MAGFRISGMASGLPPNIVEQLMDVERIPVKNMEGQKTKNEDKLK
                     LVTELETKVTDITKNLSELTSAKGFSDKKLTSGDPNIVDGTLDTGLAVPGDYSIEVLQ
                     LAQKPTAISNGFPDKNKSQIGVGYIKFKTGEGVKEVYINGSNSTLMGVAD"
BASE COUNT         3185 a         2342 c         2611 g         3154 t
ORIGIN      
        1 atttgccttg atccttttta ttcggtagcg gccgatctta ctattcatcg ccccggagaa
       61 gtgattttta ttcctcgact agtggatatc cagcttcctg atggaagcaa gcataatggc
      121 tacctcatca ttcgtgatga aggcggagcc attatcggag aaaaccgctt tgactttttt
      181 accggatttt ttgggcctta cgatcatggc aacgtgtttt cagaattggg acttggcgat
      241 aaaaaaaatc gatttggtta tcaaaaagta agtgcggagg tggcgaagat ggtgagggaa
      301 tatcggaatt atcccaatat tccctaggag aagttattac cttttccctt ttttaccgta
      361 gaattttttt ctcatatcca gagtgatctg actgataatt gttttgagtg caatctcgaa
      421 gagagtgtca gggggtattt gataaatctc agtaagcttt ctgagcgtat agataggagg
      481 atttgagagt cctctttccc aattagaaat aaactgagga gatgtataac caagtttctt
      541 ggcgatatcc aattgagaga agccagattc gattctttta ttcttcagaa aatctgcgag
      601 agatttattt tctttcatac ttagtcctgt gctctagttg ttgagtctat gttgcgtact
      661 atttgagtat gacggtcttg gtgttggtaa tcatggaaat acactattgt tgtaaatgat
      721 aagaggctgt caaaccagta aaaaaacatt ttcgagaaat ttttttattt tttgtatgaa
      781 gattgcacta ataaagggga ttcatgaaga cattattgtc tatgagtatc atgtttggtt
      841 tattcacttt agctcatgct aagacgagca tcaattccat gtgctacagg ctcgatcaac
      901 cgggatcgaa catgcaaggg gagcatgttg gggaactttt tgaaatagcc tcagtttcta
      961 aagtggtgac ttcttattgg gcaattcgcg ctttagatcc caatttcaaa ttcagtactc
     1021 acattcacat tacgccggtg ggttctcatt tttttgacgt tcatattcag ggatcacacg
     1081 atcctttttg gggacgtgag atgacgcatt ttttatttgc acagttaaat aaaattggtg
     1141 tgcatgaaat tcgtaagttg acctttgacg aaaatcttat ttttcgatgg tcagtgattt
     1201 caaactatat agatcccata aatccctctt ttcaagagat tgcggaagct ttccataatc
     1261 atttgaaaaa tttgcccaaa gagtatccta aaactcattc tgaggcggcc cttcttggtc
     1321 tctatttacc aaagatgttg tctttgaaag cccagtctgt ggcttatctt tcaagttttg
     1381 acttcaagga ggaatcacaa acggtgactt atgctttgca gtcggtgcct ttatatcggt
     1441 atctaaaaga aatgaattcc gtttcgaata accatgtggc tgaccacctt tttaattatt
     1501 tgggtggccc tgaaaaattt aaagatttta ttcagcaaga tatgaatctg aattcgaagg
     1561 atattcagtt tatcaatggt tcggggaatt cactgacggg aataaatgaa aatggagagt
     1621 cagtcaaaga atacaataag gcttcgtgtg acacgatgct aaggatcctc tttaaagtgc
     1681 gtgcacaatt gaagaaattt ggtttggatt taaaagatgt aatggcagtg gccggaacgg
     1741 atgaagggac tttgaaacct cgttacgatt ctcttcgcaa tagtctggtg gctaaaacag
     1801 gtactgtaga tcccgcagtg actttagccg gagtgatctc aacgactcag ggaaatgttt
     1861 attttggaat ttttatggag actgaaagtt ctgcggattg gagcaatgct cgtgatgata
     1921 tacgtcaaaa agttatagag ttaatggccc agtttggagg gcgtagaagt atcagctatc
     1981 ggactcgcgg gtttatgccc tttgatcaag actctggaat gattagggaa atcagacaga
     2041 gccagactca aatcaaaccc aaaccctgat ctcaaaataa gccgctttaa aaaaatcatg
     2101 tggactctca agttttaacg caatgcgccg ataagtttgc tgactgctag acaaaggtct
     2161 taccgggtta gttttgtggg cgcttttttc ctttcaaatg tctcagggcg aggcactcgg
     2221 aaaggggagg tcgcagctat gttacgtgta tcaggaatgt ccgcttcctt gatggctcaa
     2281 cgctatttgg gaacaactca acgttcgacg gaaaaagcta tgaaagattt ggcgtcggga
     2341 acccgtttca gcacagctgg aacggatgct gccggagccg ccattgctca acaactcgaa
     2401 ggacaggcca gaggccaaaa ggcggccttg aacaatgccg acaatgctac gagttttatt
     2461 caagtagccg aaggtgctct caacgaacaa aataatatcc ttatccgact gcgtgagttg
     2521 gcggtgcagg cagcaagcga tacctattcc gataaagagc gggagtatat aaattctgaa
     2581 tatacacaac tgagccagga actggaccgt atagctaaaa ccacgacctt tggagcgact
     2641 tccttgctta acggtcgctc aaagaattat gagttccagg tgggaactag cagtggagaa
     2701 gacagtatta tacgttacac ctcagacaca aacaccacgg cctccgagct gggaatcagt
     2761 gggggcagtg tagaaagcaa gagcgaggcg cgcaacgatc tcaaggatct ggatgaggcc
     2821 atggcaaaaa tcgcttcagc ccgagcgaaa tatggggcca ttcaatcacg cttagacagc
     2881 gcggctaata gtttagcgat aggggtagaa aatatcactg cagctcattc ccggatggcg
     2941 gataccgacg tggcccaggc agtgagcgac gttcgccgag ggcaaatcct tcagcagtat
     3001 caagcttcgg ttcttgctca agccaatagt agcaatgaaa ttttactacg acttctggct
     3061 tgagtttcct caaaggtcta ggttcaccta gacctaagta cataaaattt aaaaattttt
     3121 ttgcttgcat tttccagtgg ttttcaacac aagacgcgct gcgttctaga aatgctagac
     3181 ctagggccta aagttttcag gacctaggcc gagaagtatt gcatacacgt tggagctaaa
     3241 acctctggat ctttgttcta gcagtcactc gaacaaaacc tccttaggcg gattgctcgc
     3301 ctcactcaag ggtcagtcac cccaagagtg agcgcgggcc cgctccttct aaataaaagt
     3361 ttttataata catttttaca actccagcgc gcgcttcggt gggtcggaaa ccgcgttggc
     3421 ttatatccta aggaggattt catgggctta agaattcaga ccaatatggc gtcgatttcg
     3481 gcgcaaaggg ttttgtccgt tcagcagaag agaacggaac attcagctgc tgctcttgct
     3541 tcaggttcgc gcatcgtgca cgccgccgat gatgctgccg ggttagcgat ctcagaaaat
     3601 atccgcggtc aaacccgtgg gatcgcacaa gcgagaaaca atgcctttaa tgcaggctct
     3661 gcgattcaag tttctgaggg cggcttgaat gagattagta atatcttaat ccgcttgcgg
     3721 gagttaggag ttcaagcagc ttctgaccat atcggagacc gcgaacgggc attcttaaac
     3781 ttagaggcga cacaattaac tgaagaagcg gatcgtatcg ccagaaccac ggtgtttggt
     3841 gataagaaac ttctggatgg ctccggcggt aagatggagt tccaagtggg tgccttcggt
     3901 accaaagaaa atgttatcag ttatgatttg ggaagcaatg cgacggcctc agaattggga
     3961 attgagggat tgagcgttga aagtaaaggg gatgcgcgtt cggcactgca aacagtcgat
     4021 gatgccctcg tcaaagtgag ttctctgaga gcgaacttcg gtgctgttca aagtcgcttg
     4081 gattcaacag tcagcaattt ggatgtgcaa tatgaaaact tatcagcggc gaactcgcgt
     4141 atccgtgata cagacgttgc caaagaaagt tctgagatga tgtctgcgaa cgtcttacaa
     4201 caagccgcag tgtcggtgtt ggctcaagcc aatcaaacgt cagcggtggc tttgaaactc
     4261 ttatagtcat aaattttagg taggtctgtg aacgtgaaat attcacagac ctaccatgct
     4321 cttagagatt ttgtcttcaa agacaaatat ttgaggagat ggagcaaaag cttttttgca
     4381 ccaactcttg aaataaaaat taatagaact ccggtaagac gggaagttct gaaatattct
     4441 tatgaagaaa cgagtagtcg cccacaaaac cctactctta agcccgcccc gggagacgca
     4501 agtctttcgg ggttttttta aaacgcgggc taagataaat tcgataaaga ataaaatcct
     4561 gatattcttc tttttcggtc tcaaggcgta aataaagccc atccaaattt ttaaagatca
     4621 taagcggaga ttcctggtct ttactggtag tgatttttcc agcatacact ccatccaccg
     4681 gaaccagcca tcccatttgg ggtttggaaa atcctttccc taaaagagaa atagtaaccg
     4741 aagtgttgga tctgataggg ggtttagtta ttttcaaata ccagagctta ttattaattc
     4801 gatccaaggc cacccatgtg cccacaatgt cttggctttc gatgtattca cgatttacat
     4861 ccaggggcca gggaacaata ggatcgtgac cagaagctgt caccacaaag agcatggata
     4921 acagcatttg taagagcatt ttcatgcgat ttccttgtta catttattgt cagatttgaa
     4981 ctgaatagtg atttgcgggt taaggtaata gcccttacga tctttcaata gatactgagg
     5041 gctatcatca ggctctaaca aagtccgcaa tcgcttaata gagacataga taagattgtc
     5101 gtgagtaaga gagtcataag gctgctgcca aatatattcg aaaagttcac ttttcgaata
     5161 tctttttcca gggttcttaa gaaagaggag cgccatatcc atcaaaatgt gttgatttcc
     5221 aaagttgata gcccctcgtt ttttttcgag aatcaaacgc ttgtcctcat ccacttcaat
     5281 atcatggctg cggggggtgg cgtccgcgta gatttgagaa atttgccgaa agagccttgg
     5341 gtgtttatcc ttatcaacgc cacagagtgc gaggtctgca taaatctggt aaaggctttg
     5401 cctgttggca ttttgatgaa tccgcgccat ttgtgcaagg atactggaga ccacgacgtt
     5461 aaggccttgc agtttcgctg actcataggc ctcccataac aatagaagcg cttgctcagg
     5521 gtgtcccagc tcggtttgaa tataacagcg cagaagtcgc acggagatca tcagttcggc
     5581 caaaggttcc tgggaagaga gcacctcgat tttttctaga ttcacgctcg cttggggcag
     5641 atctttgtcg gaagaactgg tttttaaata ggaatcggca taaagcgcat aggcgaggct
     5701 acgtatattt tttgtttgcg aagccttgga gatagcggat gccaaataag cttgcgcttg
     5761 cgaacgatgg ccgtggttta aaagaagaat tccaagaata tattctgcag ttgccgcatg
     5821 aaagagatcc gtgttctttt ttaaaaactc aagaagttca gtttcttgtt gctccgtctt
     5881 ttcgagctgc tcaagttcga taaagcactg aatctgaatt cgcaaagcct caagccactg
     5941 agagacgtct cccagttgat gagcttcctt gcaggcctct tcgattgagt tcttcgcttg
     6001 caaaaaatca ccgctttggt attgaaggcg cgcaatttta agtgagggta aaaagttctg
     6061 agtcatgaga tgcggagttg tatttttggc ctaatttgaa gtcaagcact aacgccaaaa
     6121 gggtgcctct attgagtttt aacaccttcc tgagaagttt ttatctgcca gacccggcct
     6181 ttccactgcc cagacaaata tctttcatcg aatagtccta ggatctcccg acgaggcggt
     6241 ggtccacaga gtttttttct ggactttgat ctggaaaatt ctctctcaaa ggcttgactt
     6301 tcaaaacctt cgtccagttt gcctacgacg atcgtccaga ttcactcgac gtgactaaag
     6361 acttccgaga cctcgccgaa aggaaaggca caagcaacga caacacctcg acccggaaaa
     6421 gccgagaggg acttaaggag tgactgccga gagttcatcg ctaccaggtt tcaaggcaag
     6481 aagtgaagtc accaacgtgt ataaccaggc ggccgagtag gtacaaggcc gccaaaaaaa
     6541 atggaggttc ctgtgggaat cagaatttcg acgaacgtgg catctatcaa cgcacaacgc
     6601 acaatggccg gtggccagcg tgaaatccaa aagagcatgg ctcaattggc atcaggttcg
     6661 cgtatcacta aatcagcgga tgatgctgct ggcttagcaa tcagtgaaaa cctgaaatct
     6721 caaatgagaa gctatactca agctcaacgt aacgccaacg atggtatatc tttggtgcaa
     6781 accgcagagg gcggtttgaa tgaagtgagc aacatcttaa cccgtatgag agaactttct
     6841 atccaagcct cttctgacac tgtcggtgat cgtgaacggg gatttactga taaagaggtt
     6901 caacaactga aacaagaaat gcagcgtatc gcgcagacca ctcgttttgg aaatactaaa
     6961 cttctggatg gttctggaag tgaattcgac ttccaagtgg gaatcaataa taaagacgat
     7021 gaagatcgta ttagatatga cgcttcaatg acaaatgcac aaatagataa cttgggtgtt
     7081 gatggttttg acttcacctc caaagaaggc gctcaatccg ccctggaaaa aatcgatagc
     7141 gctcaatctc aagtgaacgg ttatcgcgcc tctttgggag ctttgcaaaa ccgtttgcaa
     7201 tcaacctctg ataacttagg tgtgatgaac gagaatatct ccgctgcgaa ctcacgtatt
     7261 cgtgacaccg atattgcggc ggccacttcg gagatgactc gaaacagtgt cctcttgaat
     7321 gcgaatacca gtgttcttgg tcaagcaaat caacttccgc aattggcttt gaaacttctt
     7381 ggataggttt gaaattaaaa actaaagagt aaatattaga gttcatttta agttttcgag
     7441 aaggtgctcc attgccttct cggtaccaac ctaccaggag ggttcttccg gagctctcct
     7501 gggtacttaa gtccggtttt tttctataaa tttaatcaat ttataggggc gccgataagt
     7561 aggttaatga atatcaaagg tcttttagga aacattttac ccaccaatcc gatacgactg
     7621 atccaaaaaa cggagaagac gatcaagtcg gattccacgt ctgatcgcga tgccaacgga
     7681 cagcaggcgg gtggcggaga gcagcagcag cgcgagccca tgaccgagga gcagctcaac
     7741 aaagccctcg aacaagtgcg aaatctttca ggagtgaaag agcacaactg gagcgtcgaa
     7801 ctgtcagtga ttgaaaatag aagatttgtt gtggtcaaag acataagcgg aaacattatc
     7861 cgccgtatcc ctgaattaga tctttggacc ttacctgagg gcaatgagtc gggtaaaggt
     7921 caaattttta agaaaaccgc ttaatccact ctccaaacca ttctaggggt ggtgtcaaaa
     7981 agtttctaaa tccgtggctc caaaattgct agacctgcac ccttgaaacc attgagaaca
     8041 ttttaggaac ggtcgatcct tactatgaag atcgtgccgg cgcgaacttc gctgcggccc
     8101 tcagcaatct ggaactatgt gatgaaaatc tgtcaaccgc aggctctctg ttaggtaaaa
     8161 aatttgatcc ctattatgcc aatgcgatcc aatcaatgtc tatgattgtg gttaaaaggg
     8221 cgcctaaatt aaataatgat ctggttcatg gcttaggttt gggcttgata aatagggttg
     8281 atctttatta tgagtcaaca gcttcgatat tgaatgcgat tgttgcttta gggacttctc
     8341 ataatgaata tgccaaggag tttgcacttt ctttactgca aaaatcggat ccttattatg
     8401 aaaataccga tactcagcta aagagcgctg tcgcatcttt aaggaacgca gccaatgccc
     8461 cctcgggaga tagctcggaa atttggagtt ttcgctcgga aaataaagtt tgtaaaacga
     8521 ttccaggcca atggggagac tattatccct gccctaaata cacagcgagt gtttcagacc
     8581 aaaaagggaa tcgaattctt ctcacttgcc gctccaatga atttggtatt aactttcgca
     8641 ttcgtccttc aagggaatta tggagtcaat tggcaaatgc aaaaatcgaa ggacttggct
     8701 ttggaactga taagatccac caatggctag ggattccgta cttgatctca gaagattttt
     8761 ctttatttgc ggaagaggcc ttatctgaag aactgcttaa tcatctgcgg tccgataagg
     8821 tgttaaacat cactcttcgc ctcaactcag gtgagtcgaa tcgctctgtt cgcttcaatt
     8881 tatccggctc aaataaggcg atcgaaaaat tgttatccta ttgccggtaa ggtgtaagcc
     8941 aggggcgcat tgctttcttg actcgtttgg aagaggttct tgtcttcgca cagctgtcga
     9001 ggggttgcgc gaagtgccta cttcttaaca ttgtagaaaa acccatgggt ctcttttgtt
     9061 cgatagaaat tatgggattt gcatttcagc gataagttct cagctctgtt actaccgcct
     9121 tgactccaag gatacaggtg atcgatttcg agttgaaaac gactggtaca acgatgtcct
     9181 tctgagccca caaattcaca gcaacctccg gcccgagcaa agacaatgcg cttgatctct
     9241 tgggagatgt atcgactggg aatgggagag tcatcgacgc tctgtttaac cgacgtcggg
     9301 gatttcagag gcggagtgcg gcctttcttt ttagctaaat gttccacggc aattattgaa
     9361 atggatttta agacttcact caagtctccg ttggggatgc tgtgactgag caaatccttg
     9421 gcttctttaa agagcttcag ggtttcttga tctagggtga gtaaaatttg cgtgtgggtg
     9481 gcgctcaagg gtttttcttg atttaacggt aagctcgcca ctggatgctg agccgttaaa
     9541 gaagctttgg cttgtttggt ggtttttccc ttaacacttt caagcagttc ttgtttttca
     9601 gtcaaactta aaggggcctc tttttccgtg cgttggatca agccctgcac cagagcggca
     9661 ttgcttaagc tcacctcgcc tttaaaaatc agttcctggg cttcggggac ggtttctatt
     9721 aattttaagg cattaagcct ttgataactc ggaccttcgc ttaagtgata gtacccaatc
     9781 agcatctcaa aaagactcga atagcccagc tttgagtaaa tccgtcgcgc tttgatttcg
     9841 cgaagactca gtaaaataat atcgcccatt cttttttccc gtttaaaaaa agagcccgtc
     9901 tgagccacca gttcctgatc ggaaagggtt tggattttct tttcaaaatc ttctaactct
     9961 tccataattt tttgatcctc caagttgtgg ttttcttagc gagtgacctc tgcgagtcac
    10021 tcgcttttat ttccttcctt aaatccaagg gcaatctatc acaggttttt aaatcttatt
    10081 tttcagcctt caaagggcct agaggagagt ttatttttaa gctaaaggcg cggggttttc
    10141 tttggcaagc agagtcttta agcggcctct aagcctttct cagggccttt ttcacaaggc
    10201 ttgcaaagtg aaaatgatga aagaggattt ccaataaaac tcctgtcaat gttttatttt
    10261 ttttaataaa aaaatgatgc gctgattagt ggatgtcggg aaatttctat gatcttcgct
    10321 cacgatcaat gatcaatgat caaggacaga tatctgcaac cagcagcccg aaaaccggga
    10381 ccagaaatta aagaccaaag attcactttc tgaagacttc ctttggagtg agggctgtaa
    10441 gtttaatttt gcttgccacc gaacttgaaa ttcaaattca aattcaaatc taaatctaaa
    10501 tctaaactca tgctttagaa gaattggttt ggaaagcttg agattccaaa cttaacccac
    10561 cgaacttttt aacataccaa atagctttca aagtgagttg cctctttatt caaaactctc
    10621 ttttattaag agagggatga ccatagtcga gcccagtgtc aaaattcccc cgtaaataga
    10681 gagttcccag ctagttttct ctcttttcga actttgtcca ggtcatttga ttgactcacg
    10741 gttttgtaaa aattctgaga gaataaaaga atcagagtat atctgatttt gaaaccagga
    10801 tggtttctaa gcacgtgtca ggaggactca agcagtggct ggctttcgta tttccggaat
    10861 ggcctcagga ttaccgccga atatcgtcga acagcttatg gatgtcgagc gcattcccgt
    10921 gaaaaatatg gaaggtcaaa agaccaaaaa tgaagacaag ctcaagctgg tcacggaact
    10981 tgaaactaaa gttaccgaca ttaccaaaaa cctaagcgaa ctcaccagcg caaaaggctt
    11041 cagcgataag aaactcacca gcggtgatcc gaatatcgtg gatggaacac tggatacggg
    11101 gttggcggtt cctggagatt acagcattga agtccttcaa ctcgcacaaa agccaaccgc
    11161 catttcaaat ggctttcctg acaaaaataa atctcaaatc ggggttggct atatcaagtt
    11221 taagaccggg gaaggcgtta aggaagttta catcaatggt tcaaattcca cactgatggg
    11281 agtggcggat ca
//
LOCUS       DATBTE010000432        10478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2313535, whole genome shotgun sequence.
ACCESSION   DATBTE010000432 DATBTE010000000
VERSION     DATBTE010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 10478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10478
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2313535"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VIG33_16490"
     CDS             <1..190
                     /locus_tag="VIG33_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272493.1"
                     /translation="IGIWRDSQNKTGPDTGVSYSITTMVSVKWRARRLRVGYPPPEAV
                     QSNQMFLAQATTKNVVKY"
     gene            402..647
                     /locus_tag="VIG33_16495"
     CDS             402..647
                     /locus_tag="VIG33_16495"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY8272494.1"
                     /translation="MPQRNVAAAHQRFSWIHSFLDGNGRVVRLFSDCFFMKENLGGVG
                     LVCGRFPEGLQEKNNLTIEHSHKLMIHEKGITTVEDP"
     gene            complement(796..1149)
                     /locus_tag="VIG33_16500"
     CDS             complement(796..1149)
                     /locus_tag="VIG33_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272495.1"
                     /translation="MKRSLMLLASILLCTGLAKATENGQAKPGLDDSILGAITLAQPT
                     AGGRLMTPAEEKTTDDQMKTQAYCYAWTTCPDGRRISCNSSGESCRWSYRTGRMVYCS
                     GTGNGRTRWVRYTCY"
     gene            complement(1282..1728)
                     /locus_tag="VIG33_16505"
     CDS             complement(1282..1728)
                     /locus_tag="VIG33_16505"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY8272496.1"
                     /translation="MIKFFLPIIAGISIVLQGTLNRNSTTYMGLTSAVLLNAIVFLFF
                     SIGAWVLFKLNLIGGSANSSVNPLVHFQWWYILPGIFGCLIVFSTPLAIKFLGANSAF
                     AIIICTQLVVSMLWDFTFEKTTPTAMSLTGVVIMIVGLFVLMAGKK"
     gene            complement(1728..2384)
                     /gene="cmk"
                     /locus_tag="VIG33_16510"
     CDS             complement(1728..2384)
                     /gene="cmk"
                     /locus_tag="VIG33_16510"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEY8272497.1"
                     /translation="MGMVITIDGPAASGKSTVSRELATRLGWQWVSTGAFYRALGYAA
                     LHSHVEITDVKALTELAHSSVWTVKMGVTRTQVFFKNHEVTDDIFHEDVGNIASKISH
                     YPEVRKALLEAQRNCSAGPQGLVAEGRDCGTVVFPQAQAKIYLTASSEHRAARRAAEL
                     GLDQGQTQEAQEVRDRQDSTRKAAPLSVPENAFVIDTTNMALDEVVDKVETYVRSKLN
                     "
     gene            complement(2386..3510)
                     /gene="hisC"
                     /locus_tag="VIG33_16515"
     CDS             complement(2386..3510)
                     /gene="hisC"
                     /locus_tag="VIG33_16515"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY8272498.1"
                     /translation="MKVSPQIVSLIPYKPGKPISETQREYGLTKVTKLASNENPLGPS
                     PKALSAIKNLLQQLHRYPDPSAFDLLQVLSRQWNFPTQQLGLGNGSDELIDILVRIYC
                     EPGEGVLTSQAAFSAYEISAQANRAKVHTVPLKSGYKFDLKAMADYFFAHPEAQIRLI
                     FIANPNNPTGTYATREEVAEFLAQLGNRDDVLIVFDEAYNEFVRAKDYANADEFMRKY
                     SNVIALRTFSKIYGLAGLRLGAMVAPPEVIEIFNRVRKPFNVNDLAQVAAIAALQDSE
                     FIAASQQIVWKGLDYFYEKLQKMGLPFIESQGNFVMFDTLRDAAQVNEALLRKGIILR
                     PIKNYGFPTQLRLSVGLEEENILAMKALEEVLAQIPKLKD"
     gene            3616..4857
                     /locus_tag="VIG33_16520"
     CDS             3616..4857
                     /locus_tag="VIG33_16520"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HEY8272499.1"
                     /translation="MAQAETDVKLILYVGESFVEAHLCRNQKSLHFNRWYLGKEGFKN
                     GLQKFLQESGVKKISKAIVASRFVEKILTYRLGGSVATLTTKGFENWPCVRQPHQKVG
                     PLSSMELMFSVDERCTAEGEITQEISDEEITELVRKLRQKLAKRICIHFLNARKNPHN
                     QNKLKEVLLKENFEVFIPEFDRENNDEVSQWRKNILNASLSSTFEEVQEELNGGLKDF
                     LPEGQRASFLSSEAILFDKESHYRLSSLWGAYGTWARTLKKQRSDKFNILYLGLEQFC
                     LLNPENQAPTWNSPWGKIQAAQVQNQLLCLQPTTSIELSPWGELSFTKTVLGYEPGPM
                     FMGRGQIPTLLDLWEEKTSNVKGIADRRSPAGVQKFKNQILALNKNSSQHLNSEEKVL
                     SHLRQLALHKMAMDIACGAQS"
     gene            4984..6333
                     /locus_tag="VIG33_16525"
     CDS             4984..6333
                     /locus_tag="VIG33_16525"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY8272500.1"
                     /translation="MLSNWIYEFADLFLKTEKSAALLSSDGDIIQAKSDLTFEITGFK
                     SIVDTSLKYIALKPGEILITNDSYCGGSFLHRYTFLMPLSRATASQPGLFICVRREFA
                     PGLNISDKVDNEGLRIPPTPIFQNGQLVTPIIEAMAMHPLCPQGFNLWLQKTVTDLGQ
                     LYTKWNQIEKNCKFQISALELKKFLSFSKKFATEKILEKAQGEARSEVRLDSGEVLKL
                     HLEINNGLIKADFSGTTTGIKNHLPDLATFGACYQAVAHFYGLNEFNNSGGFSVLQVV
                     TPLGCFLNAKYPASTHQGFQTGVAAVQLATTLALHEIVKNPQAILAETDLKMEISFSQ
                     GSRWLSLWSAKLCCESISLEQIESQYPIQFIKLEKDTEKMHLQVEFKALAPCQIRWLS
                     DFTKYPIKALRNGLKAPEPALVEMMNQENEWIALASQGNLDLIPGTHLRLNIWSLFT"
     gene            complement(6361..7563)
                     /gene="ilvA"
                     /locus_tag="VIG33_16530"
     CDS             complement(6361..7563)
                     /gene="ilvA"
                     /locus_tag="VIG33_16530"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEY8272501.1"
                     /translation="MKVTLEDIRKARETLKNVSQNTEIDLSTSASKLLGCEVYLKFEN
                     TQRTGSFKIRGAYNKIANLTSEEKKRGVVASSAGNHAQGVALSASFAGVAATIVMPEN
                     APLTKINATKGYGANVVLKGEIYDDAYEYARFLEKENGFVFVHPYEDPSIIAGQGTIG
                     LELYERLPDLDSIVIPIGGGGLISGISTAIKALNPKIKIIGVQSEQASGMSQLFHHKT
                     PHPKKRISTIADGIAIKHPSKLIYDTFISKNVDEVVTVSDDEIAEAIVFLLERAKTMT
                     EGSGAAAMAAIMNKKLSLGKKTCVILSGGNVDLNVISKIIEKGQTKRGRLVELSVVVD
                     DLPGNLNRLTKAIAEQKANILEVHHDRISQGLFLRETRIDFVLETSSPEHIERIKEAL
                     ISAGGKIL"
     gene            complement(7585..7818)
                     /locus_tag="VIG33_16535"
     CDS             complement(7585..7818)
                     /locus_tag="VIG33_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272502.1"
                     /translation="MESKVHFRRCHICGTLNEAPGQLVSNCTKCGKHLAPFHYFDECI
                     FMGLPLRKEVKPSAESILPYKSYPPIWGLTAYW"
     gene            8131..9765
                     /locus_tag="VIG33_16540"
     CDS             8131..9765
                     /locus_tag="VIG33_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272503.1"
                     /translation="MKFWALAPLVACLFSLQAFATAPRKTTRPQILLSYAQFSHLTNA
                     EQKTYIKKLREVMEEMSKAFPEFAQEMSARSSFFAQMWNSNFPAAYGEANPLASFDIG
                     KLEKYIREMDIQAKLYLDDIANTNPKKLSEENQDLYVEKYRQALFFAASAETTARHIP
                     DKKTGKYLLTRVINPTRKRVEELEAKVKPFGEIRYSQARDDYFKKAHEDNFDLNTPYP
                     LDARVPYGERLQLQKPDDLPPIAYTKDPKPNLNPTVKVDPNDKLKLPTTPRAFPKSDS
                     STVGIVSSENVSDMPPIPQSASNSEGRPPPPQLITEDQKSETPPTPKVDAKKEDQPVP
                     KAASSEETTGTAKSKKPDFGFYRCMYAGFVIKKGPDCKGPSSLPWDLEGLDTETFVCE
                     NGSIMCNPFLFGFKSSCKWDDASENNKTDTCMSSAKPYCVSKGVFATKNCSSISTSDN
                     ALQAAVQLSHKNPKAFDLFQTSFEELCDKGLIDFNGYEGKRKEKTKEDILRTCAVAQI
                     RMDALKLHYLIPKETDKIKQMMPAAEKAIKNNKTAQ"
     gene            complement(9783..>10478)
                     /locus_tag="VIG33_16545"
     CDS             complement(9783..>10478)
                     /locus_tag="VIG33_16545"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY8272504.1"
                     /translation="ADILEIWTDVAGIATTDPRLCPKARPIDEISFKEASELATFGAK
                     VLHPATLLPAIRHNIPVFVGSSFEAEARGTWVRKEVQVYPLIRAMALRKKQILVTLST
                     PEMLHAHGFLYQIFKIFNDHKVSIDAITTSEISVSVTLDDSTLLNKKLISDLSEIADV
                     QVEENLALVSLIGNNINHTPGLGKLIFETIADINVRMICLGASKHNFCFIVNEEQGAV
                     AIQKLHQKFIEAS"
BASE COUNT         3066 a         2416 c         2201 g         2795 t
ORIGIN      
        1 catcggaata tggcgggatt ctcagaataa aacaggacca gatactggtg tcagttattc
       61 aatcacgaca atggtctcag ttaagtggcg agcacgaaga ctacgcgttg gatatccacc
      121 gccggaggcg gttcagtcca accaaatgtt tctagctcaa gccactacca agaacgtagt
      181 gaaatactga gaattataaa accatccctt ttcaatttcg taaaagagct tcgtctcact
      241 ttgatatgct agactctaag ggaacgtgaa aaatgccaag cctggcccta tagcgagtga
      301 aatttcgtaa gaaattataa gaattaagac cggaaaaaag atcggaataa atgaatccaa
      361 caaagacatt tccccagctc atatcaggta tggaaccttt aatgcctcag aggaatgtgg
      421 ctgccgccca tcagagattc tcatggattc attcattctt agatgggaat ggcagagtcg
      481 tgcgactttt ttcggattgt ttttttatga aagaaaacct gggtggggtg ggtttggttt
      541 gtggacgatt tccagagggc ttgcaagaaa aaaacaatct tactatagag cactctcaca
      601 agctgatgat ccacgaaaag gggattacga cggtcgagga tccttgatat caacttaaat
      661 gaattgctca agaataaaaa taaataactt caagggacaa aagtttggat ctgagaatta
      721 actaaatttg agccctctgg cttagccgtc agaccttacc cagagcggcc caaagctcgc
      781 ttctcgaagt gggatttaat aacaagtgta acgaacccaa cgagtgcggc cattaccggt
      841 gccactgcaa tagaccattc ggcctgttcg gtaagaccag cggcaacttt caccactaga
      901 gttgcaggat atacggcgac cgtccgggca agttgtccag gcatagcaat atgcttgggt
      961 tttcatttga tcatcagtgg ttttttcttc agccggagtc atcaaccttc caccggcagt
     1021 gggctgagcc agtgtgatgg cgcccaagat tgaatcgtcc aatccgggct tcgcttgccc
     1081 attttctgtc gctttagcga gacccgtaca taaaagtatc gaagctagta acatgagtga
     1141 ccgtttcata tttcctcctt ggttagttaa acatccctgc ttagctttga attctaaaac
     1201 ccgccggttg gattggcaaa atgtaaggtt tcatcagcgt gttggatcac actcgatcgc
     1261 aggcgagtct tgaagaaaat attatttttt acccgccatc agcacaaaaa gtcccacaat
     1321 cattatcaca accccagtga gactcatcgc cgtcggcgtc gttttttcaa aagtaaaatc
     1381 ccataacatg ctgacgacaa gttgagtgca aatgataatc gcaaaagccg aattggctcc
     1441 taaaaattta atagctaagg gagttgaaaa aacaattaag cagccaaata tccctgggag
     1501 gatataccac cactgaaaat ggaccaaagg attgaccgaa gaatttgcac ttccgcctat
     1561 gaggtttagt ttaaataaaa cccaagcccc tattgaaaaa aataaaaata cgattgcatt
     1621 cagaagaacc gcagaggtca aacccatgta agtggttgaa ttccgattta acgtcccttg
     1681 aaggacaatg gagatcccgg caatgatggg aagaaaaaat ttaatcatta attcagtttt
     1741 gagcgaacat aggtttctac tttatccaca acctcatcca aggccatatt ggtcgtgtct
     1801 atcacaaagg cattctcagg cacacttaaa ggcgccgctt tgcgagtgga atcttggcga
     1861 tcgcgcactt cttgggcttc ttgagtttga ccttgatcaa gtccaagctc tgccgcccgc
     1921 cgagcggccc gatgctcact ggaggcggtc aaatagattt ttgcttgagc ttgcggaaat
     1981 accactgttc cacaatcacg tccttctgcg actaagcctt gaggtccggc actgcaattg
     2041 cgctgcgcct caagaagagc ttttctgact tcggggtaat gagaaatctt gctggctatg
     2101 ttgcctacat cctcgtggaa aatatcatct gtaacttcgt gatttttgaa gaaaacttga
     2161 gtgcgagtca ctcccatttt cacggtccaa acagaggagt gagcaagctc cgttaaagct
     2221 ttaacgtcgg taatctcaac atgagagtga agagccgcat agcccaaagc acgataaaaa
     2281 gcccccgtag aaacccattg ccatcctaaa cgagtggcta actcacgact taccgtggat
     2341 tttccagaag ctgcaggccc atcgattgta attaccattc ccatttcaat cctttaactt
     2401 tggtatctgc gctaaaactt cttccaatgc cttcatcgct aaaatgttct cttcctcaag
     2461 gcctacactc aagcgcaatt gagtggggaa accataattc ttgatcggtc ttaagataat
     2521 ccccttgcgc aataaagctt cattcacttg agctgcatcc cgaagggtgt caaacattac
     2581 aaaattacct tgagattcaa tgaagggcag tcccattttt tggagcttct cataaaagta
     2641 atcaagccct ttccaaacaa tctgttgcga cgctgcaatg aattcagaat cctgcaaagc
     2701 ggcaatggct gcgacttgtg ctaaatcatt tacattgaag ggtttccgca cacgattaaa
     2761 aatttcgata acttctggcg gagcaaccat ggctcccaga cgaagtcccg ccaagccata
     2821 gatttttgaa aaagtccgaa gggcaatgac atttgaatac ttacgcataa attcatccgc
     2881 atttgcataa tccttcgctc gaacaaactc attgtaagcc tcatcgaaaa caatcaacac
     2941 atcgtcccga ttccccagct gagccaaaaa ctccgccact tcctcgcgag tcgcataagt
     3001 gcctgttgga ttgttgggat ttgctataaa tatcaggcgg atttgagctt caggatgagc
     3061 aaaaaaataa tctgccatag ccttgagatc gaatttataa cccgatttca agggaactgt
     3121 atggactttg gcgcggttgg cctgagcact aatttcatag gcggaaaagg cggcttgtga
     3181 agtcaaaacg ccctcccctg gttcacaata aatgcgcacc agaatatcga taagctcatc
     3241 cgaaccattt cctaaaccca attgctgagt tgggaaattc cactgtctgg aaaggacctg
     3301 aagcagatca aaggcagaag gatctggata acgatgcagt tgttgtaaaa gattcttaat
     3361 tgctgataaa gccttaggac tgggtcccag aggattttcg ttacttgcga gtttagtaac
     3421 tttcgtaagt ccgtattctc gttgagtttc tgagatgggc tttcccggtt tataaggaat
     3481 taagctaaca atttgtggtg aaactttcac aggggcccct ttcggactac cttttaaggt
     3541 ccaatttggt tatatttggc cctcgatttt ttacaactta gctttaacaa caatcgtata
     3601 gatattcaag gactcatggc tcaagcggaa acggatgtta aattaatact ctacgttggt
     3661 gaatcttttg tagaagctca tctctgtcgt aatcaaaaat cccttcattt taatcgctgg
     3721 tacctcggaa aagaggggtt taaaaacggt ctgcaaaagt ttttgcaaga atccggcgtt
     3781 aaaaaaataa gtaaagccat tgtcgcctcg cgctttgtcg aaaaaattct cacctatcgg
     3841 cttgggggga gcgttgccac cttaaccact aagggctttg aaaattggcc gtgtgtacgc
     3901 caacctcatc aaaaagtggg tcctctttcc tctatggaac tgatgttttc cgtggatgaa
     3961 cgctgcacgg cggaaggtga aatcactcag gaaatttcgg atgaagagat aacggagctt
     4021 gtgagaaaac tccgccaaaa actggccaaa cgaatttgta tccatttttt aaatgcgcgt
     4081 aaaaatcccc acaatcaaaa taagcttaaa gaagttttac tgaaagaaaa cttcgaagtc
     4141 ttcattccgg aatttgacag ggaaaacaac gatgaagtgt cccagtggag aaaaaatatt
     4201 cttaatgcgt ctttgagtag cacttttgaa gaggtgcaag aggaactcaa tgggggctta
     4261 aaggatttct tacctgaagg gcaacgggcg tcttttttat catcagaggc catcctcttt
     4321 gataaagaaa gccattaccg cctgagttct ctttgggggg cctatgggac ctgggcacgc
     4381 actcttaaaa agcaacgctc tgataagttt aatattcttt atttgggcct tgaacaattt
     4441 tgccttttaa atccggaaaa ccaagctcca acttggaaca gcccgtgggg aaaaatccag
     4501 gctgctcaag tgcaaaatca attgctttgt ttgcagccga ccacgtctat tgaactgagc
     4561 ccttggggcg aactctcctt tacaaagacg gtcttgggct acgagccagg cccgatgttc
     4621 atggggcgag ggcaaatacc cactctcttg gatttgtggg aagaaaaaac cagcaatgta
     4681 aaagggatcg cggacagacg ttcaccagca ggcgtacaaa aatttaaaaa ccagatcctc
     4741 gccctcaata aaaactcttc tcagcactta aattctgaag agaaagtgtt gtcgcatctt
     4801 cgccagctcg cccttcataa aatggcgatg gacattgcct gtggcgcaca gagttaaaaa
     4861 taatttgcat gggagcttta gccccgctgt ttggagctga actcaagaaa agattgcccc
     4921 atcttcaata tgaatttctt cccgaaactg aaacttcttt tttattaagt cagggccacg
     4981 atcatgctct ctaactggat ctacgaattc gcagacttat ttctaaaaac cgagaagagc
     5041 gctgccttgc tttcgagtga tggtgacatc attcaagcga aatcggacct tacctttgaa
     5101 atcacgggtt tcaaaagcat tgtcgacact tcgcttaaat acatcgccct aaaacctggc
     5161 gaaattttga taaccaatga ctcctattgc ggtggcagct ttttgcatcg atacaccttc
     5221 ttgatgcctc tctctcgcgc aaccgccagc caacccggcc tatttatttg tgtgcgccgt
     5281 gaatttgctc ccggcctcaa tatcagcgac aaggtggaca atgaaggcct tcgcattcca
     5341 ccgactccga tatttcaaaa cggtcagctc gtgactccca tcattgaggc tatggccatg
     5401 catccactat gtcctcaagg ctttaatctc tggttgcaaa aaaccgtgac tgatcttggt
     5461 caactttata caaagtggaa ccaaattgaa aaaaactgta aatttcaaat cagtgcactg
     5521 gaactcaaga aattcttaag tttttctaaa aagtttgcga ctgagaaaat tcttgaaaaa
     5581 gcccagggcg aagctcgttc tgaagttcgt ttagacagtg gtgaagttct gaaacttcac
     5641 ttagagatca ataacggcct tatcaaggcg gattttagcg gtaccaccac tggaattaaa
     5701 aaccacctcc ctgatcttgc cacttttggc gcctgttacc aagccgtggc tcatttctat
     5761 ggtctcaatg aatttaataa ttcaggaggc ttttcagtcc ttcaggtggt cacgcctttg
     5821 ggctgttttt tgaatgccaa atatccagcc tccactcatc aaggttttca gaccggtgtc
     5881 gccgccgtac aactggctac aaccttagct cttcatgaaa ttgttaaaaa tcctcaagcc
     5941 attttggcgg aaactgattt aaaaatggaa atttcatttt cacaaggttc ccggtggttg
     6001 agcctttggt ccgccaaatt atgctgcgaa agtatttctc tggaacaaat tgaatcacaa
     6061 tatcctattc aatttataaa acttgaaaag gacacggaga aaatgcattt gcaggtcgag
     6121 tttaaagctc tggccccatg ccaaattcgg tggttatcgg attttaccaa atatcctatc
     6181 aaagccttac gcaatggttt gaaggctccg gaacctgctt tagtagaaat gatgaatcag
     6241 gaaaatgaat ggatcgcctt ggcttctcaa ggcaacctgg atctgattcc aggcacccac
     6301 cttcgattga atatttggag ccttttcact taagaagtaa agactcccct acaaatagac
     6361 ttagagaatt tttccgccgg cagagatcag ggcttcttta attctttcaa tatgctctgg
     6421 actgctggtc tcaagtacaa aatcaatgcg ggtttcccgc agaaaaagtc cctgagaaat
     6481 acgatcatgg tgaacttcga ggatattggc cttttgctcg gcaatcgcct ttgtcaaacg
     6541 attgagattt cccgggaggt catccaccac tacggaaagt tcgaccaaac ggccacgctt
     6601 ggtctgtccc ttttcaatga ttttcgaaat gacgttcaaa tccacattgc ccccacttaa
     6661 aatcacacag gttttttttc cgagactgag ctttttattc atgatcgccg ccatcgccgc
     6721 agctcctgat ccttcagtca tggtttttgc tcgctctaac aaaaacacga tcgcttccgc
     6781 gatctcatca tcgctcacgg tgacgacttc atccacattc ttagaaataa aagtatcgta
     6841 gataagcttc gagggatgct tgatcgcgat cccatccgcg atcgtcgaaa tacgtttttt
     6901 gggatgagga gttttatgat gaaagagctg cgacatccct gatgcctgct cagattgcac
     6961 gccgataatc ttgatttttg gattgagcgc tttgatcgcg gtggaaatac cgctgatcag
     7021 cccgccaccg ccgataggaa tcacaataga atccaaatct ggcaatcgct cgtaaagttc
     7081 taagccaata gtgccttggc cggcgatgat agagggatcc tcataaggat gaacaaaaac
     7141 aaaaccattt tctttctcca gaaagcgcgc gtactcgtag gcatcgtcat agatctctcc
     7201 tttcaaaaca acattggccc catagccctt ggttgcattg attttagtga gcggtgcatt
     7261 ttctggcatc acgatagtgg ctgcgactcc cgcaaaggat gcacttaagg cgacaccttg
     7321 ggcatggttt cccgcggagc ttgcgaccac gccccgtttt ttctcttccg aggttaaatt
     7381 ggcgatttta ttataggcgc cacggatctt aaaactgccg gtgcgctgag tattttcaaa
     7441 ttttaaatac acctcacaac caagaagttt gctggcactg gttgataaat caatttctgt
     7501 attttgcgag acgtttttta aagtttcgcg ggccttgcga atatcttcaa gagtaacctt
     7561 catgtaaaaa accttaaaca ctttctacca gtaagccgtc agtccccaaa tcggtggata
     7621 agatttatag ggaagtattg attctgcact aggctttacc tcttttcgca gaggcaaccc
     7681 cataaagata cattcgtcga aataatgaaa gggtgcaaga tgcttcccgc atttggtgca
     7741 gttagaaacc aactgcccgg gagcttcatt aagagttcca caaatatgac aacgcctaaa
     7801 atgaactttt gattccatag tgtttttgtt tccttcctct tgatgttcac ccctacactt
     7861 tggtcttgca agagggagag ctaaagctaa gatattgaaa tctcacaaaa tagcgtaaat
     7921 tgacttttcg cgtcacgcac aatttgctga ggtttcggaa gcttgatgta aggtgccaaa
     7981 attgttcgat tttataaaaa taaactcctt tacatccctt actgatacgc gagcgggcct
     8041 aagtcctgaa gtatgacttc tcctagggaa attccggcga aagctttaaa ataaacaacg
     8101 ggataggttt tttattgaaa ggttcaaata atgaaatttt gggctctcgc tcctttagta
     8161 gcgtgtcttt tttctctgca agcattcgct accgctcccc gcaagacgac ccgcccgcaa
     8221 attctgctca gctacgccca gttctctcat ctcaccaatg ccgaacaaaa aacctacatc
     8281 aaaaaattgc gtgaagttat ggaggaaatg agcaaagcct ttccagagtt cgcccaggaa
     8341 atgtccgcgc gttcgagttt ctttgcacaa atgtggaatt cgaatttccc cgcagcctac
     8401 ggtgaagcca atcctctagc ttcctttgat atcggcaagc tcgaaaagta tattagagaa
     8461 atggatatcc aagcaaagtt atatttagac gacattgcca atacgaatcc caaaaaacta
     8521 tcagaagaaa accaagacct ctatgtcgag aaatatcgac aagctctctt ttttgctgct
     8581 tccgcagaaa ctacagctcg acatattccc gataaaaaga caggtaagta tctcttaact
     8641 agagttataa atcccactag aaaaagagtt gaagaacttg aagcgaaggt aaagccgttt
     8701 ggcgaaatta gatattcaca agctcgtgat gattatttca aaaaagccca tgaggacaat
     8761 tttgatctca acacacccta tccgttagat gcccgtgtgc cctacggcga acgcttgcaa
     8821 ctgcaaaaac cagatgatct cccccccatt gcttatacga aggatcctaa accaaattta
     8881 aatcccacag tgaaggtgga ccctaacgat aaattaaagc ttcctacaac tcctagagct
     8941 ttccctaaat cagattcatc tacagtcggc attgttagct cagaaaatgt ctcagacatg
     9001 cccccgattc ctcaaagtgc ttccaactca gagggacgtc ctcctcctcc acaattgata
     9061 actgaagacc aaaaatcaga aactcctccg acccctaaag tggatgctaa aaaagaggat
     9121 cagcccgtac caaaggcggc ctcttcagag gaaaccacag ggactgctaa atcaaaaaaa
     9181 cccgattttg gtttctatcg ttgtatgtat gcaggctttg tcattaaaaa aggtcctgat
     9241 tgtaagggac cgagttctct tccttgggat cttgagggac tggatacgga aacttttgtc
     9301 tgcgaaaatg gcagcattat gtgcaaccca ttccttttcg gatttaagag cagctgtaaa
     9361 tgggatgatg ccagcgaaaa caacaaaaca gatacttgca tgagttccgc gaaaccttac
     9421 tgcgtaagta aaggggtttt tgctacaaaa aattgctcct ctatcagtac aagtgacaat
     9481 gcacttcaag cagccgtcca actcagtcat aaaaatccaa aagcgttcga cctgttccaa
     9541 actagcttcg aggagctttg tgataagggc ttaattgact tcaacggcta cgaaggaaag
     9601 cgcaaggaaa agacgaaaga ggatatcttg agaacttgtg cggtcgctca aattcgcatg
     9661 gacgcattga aattacatta cttaattcca aaagagactg ataaaatcaa gcagatgatg
     9721 cccgccgccg aaaaagctat aaagaataat aaaaccgcac aatagccctc ttataaggga
     9781 tgctatgacg cttcaataaa cttctgatga agtttttgaa tggccacagc tccctgttct
     9841 tcattgacga taaaacaaaa attatgtttg ctggcaccta agcaaatcat tcgcacattg
     9901 atatccgcaa tggtctcgaa aatcagtttt cccagacccg gcgtatgatt gatgttattg
     9961 ccgattaagg aaaccaatgc caaattttcc tcgacttgaa catcggcaat ctctgaaaga
    10021 tctgagatca actttttatt caacaaagtg gaatcgtcca aagtcacgct gactgaaatt
    10081 tcagaagtgg taatggcatc aatactgact ttatggtcat taaagatttt aaaaatctga
    10141 tataagaaac catgggcgtg caacatttca ggcgttgaaa gcgtcaccag gatctgcttc
    10201 ttacgaagag ccatcgctcg gatcaaagga taaacctgga cttcttttcg tacccaagta
    10261 ccgcgggctt cggcctcaaa gctagagcct acaaataccg gaatgttgtg acgaatcgcc
    10321 ggcaaaagcg tcgcgggatg caaaaccttc gctccaaaag tagccagctc ggaagcctcc
    10381 ttaaaggaga tctcatcaat aggtctggcc ttcgggcaaa gacgtggatc ggtggtggcg
    10441 atgcctgcca catccgtcca aatttcgagg atatccgc
//
LOCUS       DATBTE010000433         3999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2107861, whole genome shotgun sequence.
ACCESSION   DATBTE010000433 DATBTE010000000
VERSION     DATBTE010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2107861"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VIG33_16550"
     CDS             complement(<1..218)
                     /locus_tag="VIG33_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliP"
                     /protein_id="HEY8272505.1"
                     /translation="MKKILLLVGLVFFGMLGQQVAFAQQVTLPTVNLGFKTTENPNEV
                     VNAIKLVLIMTVLTLAPAILIMMTGFTR"
     gene            complement(215..802)
                     /locus_tag="VIG33_16555"
     CDS             complement(215..802)
                     /locus_tag="VIG33_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEY8272506.1"
                     /translation="MRLIIITALLTTLLAIPSVKAENASTPAVEEMTATDKIENKKES
                     EIPLNLQSPKKASEDGHSIFKIILIFSMMGLVGTGAYIYMRKYAKNNFAAGKNNQIKV
                     LTQHYLGPKKSLAIIRVAGESILIGVTDNNINMIKSLALLDDEIPEEVHSSSFSDVFG
                     GVPAHVSEENDEEEFSIRGIKEVVSKKLKGMRSFQ"
     gene            complement(814..1218)
                     /gene="fliN"
                     /locus_tag="VIG33_16560"
     CDS             complement(814..1218)
                     /gene="fliN"
                     /locus_tag="VIG33_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEY8272507.1"
                     /translation="MDDNLENLADQLAEEAAAMNNALAGKTGGGGGGAAGSGAGSDGS
                     GGPKDKNLQLILDIPLRVTVELGRTKMPVSELLNLTQGSVIELSKLAGEPMEVYVNEK
                     LIARGEAVVVNEKFGVRLTDIISPTERIEQLK"
     gene            complement(1230..2231)
                     /gene="fliM"
                     /locus_tag="VIG33_16565"
     CDS             complement(1230..2231)
                     /gene="fliM"
                     /locus_tag="VIG33_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEY8272508.1"
                     /translation="MNQVLSQSEVDALLAAVSDGDVTTADNSSASNPGTVKTEGKNVV
                     NYDLTSQDRIIRGRLPQLEVIYEKFMRAFRVSLSSTLRKIASITLTSTEFLKFGEFIN
                     TLPMPTCMSVLRFGTLRGSALMVIESKLAYALVDSFFGGADRPYTKVDGKEFTQIELS
                     IVRKVVELAINDLENAWASVEKIDCSFVRTEVNPQFVGIVPPTDVVIASTFDVELENA
                     TGTISIVVPYATIEPIKQKLQSGFQVESDQTDKKLWTAIIKEQLLETNVEVKVNLGQT
                     EILLQDLMTMKLGDVIPLDQDASGEIDVQIENVKKFKGYYGIHHGTVAVQVTRQIKK"
     gene            complement(2241..2810)
                     /locus_tag="VIG33_16570"
     CDS             complement(2241..2810)
                     /locus_tag="VIG33_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEY8272509.1"
                     /translation="MEVAEEKGAAAAATSAAATAAPLGGAGGGKNNIILIALAIVNML
                     VVGGVGFMLFQGRKKEAAEPKIEQVIKGEKEAQEHDKENTDEKKFVGQIIPLEKFIIN
                     LAGSKGRKLASVSIELEVKGSAVQEEIDKRKAQIRDIIIIILSSKTYEEVSSREGKDN
                     LRNEVKDTINSFLVKGKISNVFFTEFIYN"
     gene            complement(3305..>3999)
                     /locus_tag="VIG33_16575"
     CDS             complement(3305..>3999)
                     /locus_tag="VIG33_16575"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEY8272510.1"
                     /translation="ADKESEKVISDYLRKNFPSIDFLGEESNYLGAKVPWTSAGPEGR
                     WILDPLDGTTNYIHQFPIFCISLALEINGEIQVAVIDCPKMEETYTAIRGKGAFMNGQ
                     PLKVGQAQHLKDSLLATGFVAEKQDVIEEQLKIFSDIVYKCRGVRRPGAAAYDLAMVA
                     RGVFDGFWERNLQPWDSAAGILLIEEAGGKVATYQGKKYTPYKNSIIAGNPLVVDELI
                     KTLAPYLSKETD"
BASE COUNT         1078 a          978 c          762 g         1181 t
ORIGIN      
        1 atacgagtaa agccggtcat catgattaaa atcgccggtg ccaaagtcag taccgtcatg
       61 atcaacacaa gcttaatcgc atttaccact tcatttggat tctcggttgt tttaaaaccc
      121 aggttcacag tcggtaatgt gacctgctgg gcaaaagcca cttgctggcc cagcatccca
      181 aaaaaaacca aacctactaa aagtaatatt tttttcactg aaaagacctc atgcctttca
      241 gctttttaga gaccacttct ttaatgccgc ggattgaaaa ttcttcctca tcattttctt
      301 ctgagacatg agcgggaact ccaccaaaaa catcggaaaa actgctacta tgaacttctt
      361 ccggtatttc atcatccaac aaagccagcg atttaatcat attaatgttg ttgtcagtaa
      421 caccgatcag gatgctctcc ccggcgaccc gaatgatcgc taaggatttt ttaggtccta
      481 ggtagtgttg agtgagcact ttgatttgat tatttttacc ggctgcaaaa ttattttttg
      541 cgtacttgcg catgtagatg taagcgcctg tgcccactaa tcccatcatt gaaaaaatca
      601 aaataatttt aaaaatcgaa tgaccgtcct ctgaagcttt tttgggggac tgcagattca
      661 aagggatctc ggattctttt ttattttcaa ttttatcagt cgctgtcatt tcctcgacgg
      721 ctggagttga ggcgttttcg gcttttaccg atggaatcgc taatagtgtt gttaaaagtg
      781 cagtaataat aatcagacgc atttttttaa cctctacttc aattgctcaa tacgctcggt
      841 cggactaata atatctgtta agcgcacacc aaatttttca ttgactacga cggcctcacc
      901 acgagcaatg agcttttcat tcacataaac ttccatcggt tccccggcaa gtttactcag
      961 ttcgatgacg ctgccttgag taaggtttaa caactcactt acgggcattt ttgtccgccc
     1021 caattccacc gtcacacgga ggggaatatc taaaatcaat tggagatttt tatctttggg
     1081 accaccgctt ccatcactac cggcacccga tcctgcagcg ccaccgcctc cgccgccggt
     1141 tttgcccgcg agggcattgt tcatcgcggc tgcctcttca gcaagttgat ccgctaaatt
     1201 ctctaaatta tcatccatgg cttttccccc tatttcttaa tttgacgagt gacctgcacc
     1261 gccaccgtcc catgatgtat accgtaatag cctttaaatt ttttgacatt ttcaatctgc
     1321 acatcgatct cgccgctagc gtcttgatcc aaaggaatca cgtcacctaa tttcatggtc
     1381 ataagatctt gaagaagaat ctctgtttgt ccaagattga ctttgacttc aacgttggtc
     1441 tccaagagct gttccttgat aatcgctgtc caaagttttt tatctgtttg atcggactct
     1501 acctgaaatc cagattgaag tttttgtttg ataggctcaa tggtggcata aggcacgact
     1561 atggagatag tccctgtggc attttcgagt tccacgtcga aagtcgaagc gatgaccacg
     1621 tctgtaggag gaacaatacc tacgaattga ggattgacct ccgtacgaac gaaactacaa
     1681 tcgatttttt ccacggaagc ccaggcattt tccaaatcat tgatggccaa ctccacgact
     1741 ttccgcacga ttgaaagttc aatttgcgta aattctttac cgtcaacttt agtataagga
     1801 cgatccgctc ctccgaaaaa actatccact aaggcataag ccaatttact ttcgatgacc
     1861 attaaagcgg agccgcgcaa agttccaaaa cgtaaaacac tcatgcaggt aggcatcggt
     1921 aaagtattga taaattcgcc gaatttcaaa aactcggtgc tggtcagcgt aatcgaagca
     1981 attttacgta aagttgaaga taaggaaacc ctgaaagctc gcatgaattt ttcataaata
     2041 acttccagtt gtggcaaacg accgcgaatg atgcggtctt ggctcgtcaa atcgtaatta
     2101 acgacgtttt tgccttccgt cttcaccgta ccaggattgc tcgcactcga attatcagca
     2161 gtcgtcacgt ctccgtccga aacggcggct aagagcgcat ctacttcact ttgcgacaag
     2221 acctgattca tgctcgacct ctagttatag ataaattctg taaagaacac gttggaaatc
     2281 tttcctttaa ccagaaaaga attgatcgtg tctttcactt cattacggag attgtcttta
     2341 ccttcccttg aagaaacctc ttcgtaggtt ttactggaaa gaataatgat aatgatgtcg
     2401 cgaatttgag ctttgcgttt atcaatctcc tcttggaccg cagacccctt cacttctagt
     2461 tcaatactca cactcgcaag tttacggccc ttggaaccag ccaaattaat aatgaatttc
     2521 tctaaaggaa tgatttgtcc cacgaacttc ttttcatcgg tgttctcttt atcgtgttcc
     2581 tgagcttcct tttccccctt gatcacttgc tcgatctttg gctctgcagc ttcttttttt
     2641 cttccttgaa aaagcatgaa acctacaccg ccaacaacca acatattgac gattgcaaga
     2701 gcaatcagaa tgatattatt ttttccgccg cccgcgccgc ctaatggcgc tgccgtagct
     2761 gccgctgacg ttgccgccgc tgccgctcct ttttcttctg ccacttccat cctccttgag
     2821 gaaaagacac accgatctta taaaagtatg aagcaacagg agtgccaact caccattgac
     2881 caagtctagc cactaatttg gcgctcgaaa tgggttataa aagaaatgag gttttttttt
     2941 ggaaagatct ggacctcgct cctaccaaaa taccgatgcc tgagtcatcg ttttgacggc
     3001 attttaaaat accccgacca tctcccgcag cctttgaaaa attaggatgt ccttcatttc
     3061 gctggaagaa agaatttttt gtatttctgg aggaactgta tattttccag ttttgcagga
     3121 tgagaaccaa tgcaagacag gaaatttccc gcctctttat cattggcaca aaaggtcggc
     3181 gcaagcgcct cctgtgcctt ccggggcaag agaataatta acaatatgaa aattaagctg
     3241 aggccgaaga aaaaaaacca ggagtgtttc ataattccgt cctttcttca ctcatcgccc
     3301 ttacttaatc ggtctcctta cttagataag gtgccaacgt ctttataagt tcatcgacca
     3361 caagagggtt gcctgcgatg attgaattct tgtaaggagt atacttcttt ccttggtagg
     3421 tggccacttt tccgcccgct tcctcaataa gtaatatgcc tgcggcgcta tcccaaggtt
     3481 gtagattccg ttcccagaat ccatcgaata ctccacgtgc caccattgcc aagtcgtaag
     3541 cggcagcgcc tggtctgcga accccacggc atttataaac aatatcagaa aatattttta
     3601 actgttcttc gatgacatcc tgtttttctg caacgaatcc tgttgctaat aaagaatctt
     3661 ttaaatgctg agcctggcct actttcaagg gctggccatt cataaaagcg cctttgccgc
     3721 gaatagcggt ataggtttct tccatcttgg gacagtcaat cactgccacc tgaatctcac
     3781 cattaatttc aagagccaga ctgatgcaga aaattggaaa ttgatgaata taattagtcg
     3841 ttccatccaa aggatctaaa atccaacggc cttcaggccc ggctgaagtc caggggactt
     3901 tagcccccaa gtaattcgac tcttcgccca aaaagtctat acttggaaag tttttcctaa
     3961 gataatcaga aataactttt tcagattcct tgtcagcct
//
LOCUS       DATBTE010000434         6899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1698854, whole genome shotgun sequence.
ACCESSION   DATBTE010000434 DATBTE010000000
VERSION     DATBTE010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698854"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..263)
                     /locus_tag="VIG33_16580"
     CDS             complement(6..263)
                     /locus_tag="VIG33_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272511.1"
                     /translation="MKSFVITLLTTLSLSALAAPPSTTEYTPEQRKQMAEMHSKAASC
                     LNSNKTIQECHNEMMKSPGGQWGCPMMGTGPQPDTKTKTTK"
     gene            411..698
                     /locus_tag="VIG33_16585"
     CDS             411..698
                     /locus_tag="VIG33_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY8272512.1"
                     /translation="MPMIYFKKNKPSIKVAMGATLMRSLLEAGVPVASSCNADGVCAK
                     CGIRIIQGMENLSPANQTELFLKEKNNISVDIRISCQTQVWGDIVVDADYW"
     gene            805..1752
                     /gene="trxB"
                     /locus_tag="VIG33_16590"
     CDS             805..1752
                     /gene="trxB"
                     /locus_tag="VIG33_16590"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEY8272513.1"
                     /translation="MMDQKIENLIIIGSGPAGLTSAIYSARARLEPLMIEGEEAGGQL
                     MITTEVENYPGFEHGITGPELIDVMRKQALRFGTRFVSRNVTKVDFSQRPFKVWVGDK
                     LYLSKAIIISTGASAQYLGLPSEKQYLSRGVSACATCDGAFFRNMDVGVVGGGDTAME
                     EAQFLTRFAKKVYVIHRRDHFRASKIMSERTLSNPKIEVLWNSEVSEVLGDGKNVTGL
                     RIKDTLKSSTKDLAVDGVFIAIGHKPTTSLFKGLLDMNETGYLVTKANSTYTNIEGVF
                     AAGDVQDHVYRQAITAAGTGCMAAIDCERWLETQAHGLK"
     gene            1757..2254
                     /locus_tag="VIG33_16595"
     CDS             1757..2254
                     /locus_tag="VIG33_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY8272514.1"
                     /translation="MKSKKINTKDLINKIEKVAKARIASQHVVSLADFREAVKKEEPR
                     VILVIEDDETMRMALKRIFEAEGYVTKLAADATALSEVLDDTPIDLILMDVGLPWVNG
                     FELAQLLKENKDLKGIPLVFVSGKASEEDMKQAFEIGADDYIRKPFDVEKLRKTVRTL
                     LKLNE"
     gene            complement(2281..3081)
                     /locus_tag="VIG33_16600"
     CDS             complement(2281..3081)
                     /locus_tag="VIG33_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY8272515.1"
                     /translation="MKTEGKIWIIFDSAANKKTKPLSVVQTQMMILTFKMRQLHQYYI
                     WTPGWTEWLPLTQFLSSGQKYFVQAQPPEPLINKEKQKSSDVTVKIQRIASEAPSYTS
                     VVAAEKLETSDYGYYYNEFKGDDLTLSGLPDKPSVEIVVSRSELNTPKDRRTAHRHDF
                     KIEAVLLTKRGSSFRTHSRNISLSGTLLEDEIPKDFFHRPFEMILVNKFEKDTKKNHV
                     HLKGKIIGDIADPRRLVFLDQDEETTLKLSKLINDYMAQQNRLKKDAG"
     gene            complement(3190..4158)
                     /locus_tag="VIG33_16605"
     CDS             complement(3190..4158)
                     /locus_tag="VIG33_16605"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEY8272516.1"
                     /translation="MSHYLLEVENLVKTFPINGGILNREIASVKAVSGVNFKLKKGET
                     LGLVGESGCGKSTLGRCLIRLLDPTSGTVRFNGQDITHIEGDELREIRRKMQIIFQDP
                     YASLNPRMTIGSILEEPLIIHNLYPSEKARKERVMELAQLVGLRPEAMNRYPHEFSGG
                     QRQRVGIARALAVNPELIICDEPVSALDVSIQAQVLNLLMELQKKLGLTYVFIAHDLK
                     VVEHVSTQVAVMYLGKIVEMSESEELYTHPKHPYTRALLSAIPVPDPKHKEERIILTG
                     DVPSPVHPPAGCHFHPRCPMAIEDCKKTVPPLEEKRPHHVAACIRV"
     gene            complement(4155..5150)
                     /locus_tag="VIG33_16610"
     CDS             complement(4155..5150)
                     /locus_tag="VIG33_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8272517.1"
                     /translation="MNETLLEVKSLKTQFKTDDGTFYAVDDVSFTVKKGQTIGIVGES
                     GCGKSVTSLSIMKLIQSPGRIESGEILYHGKDLLKTSEEEMRHIRGNEIAMIFQEPMT
                     SLNPVYTCGNQISESISLHYPQFTKAEVKARAIEMLRIVGIPAPEQRYNEYPHQLSGG
                     MRQRVMIAMAISCDPQLLIADEPTTALDVTIQAQILDLMRKLQKDFNAGMILITHDLG
                     VVAEMCQEVVVMYAGKVVEYGTVEDIFYRPKHPYTRGLLDSIPHFETGHKLTQLKTIQ
                     GMVPSLLALPKGCRFQDRCPNVQNDCRQVEPRLESKQGAHLAACFHPVPDQGDKL"
     gene            complement(5254..5928)
                     /locus_tag="VIG33_16615"
     CDS             complement(5254..5928)
                     /locus_tag="VIG33_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEY8272518.1"
                     /translation="MTWPLLIFDLDGTLIDSAKDISSALNKTLTFYNKSTLPHEVIVN
                     HIGEGLLKLLSDFFPEHKNSPPHEYQHIYLHFLNTYEEEMHKTTRLYPGAVEFLRQYP
                     GPKGIVTNKNEAPAKKVLHHLNLLAHEEISWVGVFGADTFSECKPHPLPLQEMMKLAK
                     HSKTTTFMIGDGIPDMKAAQAAGVGAIAVEFGYSKIEILQKYNPVATLPSYATLHSLI
                     KELMSR"
     gene            6015..6692
                     /locus_tag="VIG33_16620"
     CDS             6015..6692
                     /locus_tag="VIG33_16620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEY8272519.1"
                     /translation="MLSMGMRIEIPQAQEYIESLAAVEAASKQKSRRFAEELGLGKIC
                     LSPSEGHLLSFLVRMQRCRKFVEIGTLTGLSAQYILEGLGEQGELWSLEKTELHAQKA
                     LAALAEHPAAKNVHIIVGDARVTLETLKAQGPFDGVFIDGNKAAYGDYLAWAEANVSP
                     GGLIIADNVFLNGAVWGDKEGQSQKFSEKQVKVMQTFNHRLSDPVLYDSVLLPTKEGM
                     HFAVKKF"
BASE COUNT         1891 a         1391 c         1630 g         1987 t
ORIGIN      
        1 tgaaactact tggtggtttt agtttttgta tctggctgag gaccggttcc catcatagga
       61 cagccccatt ggcctcctgg acttttcatc atttcattgt ggcactcttg gatggttttg
      121 ttggagttga ggcaggaagc agctttactg tgcatctccg ccatttgctt gcgttgttcc
      181 ggagtatatt cggtagtact cggtggagct gccagtgcag acaagctaag agttgtgagt
      241 aacgtaatta cgaatgactt cataaaaacc ccctaagttt ttgaacaatt cctttatagt
      301 tgatcggtat ggactgaata tgattataag agaaaattct ttctaattaa aatccttaag
      361 cggtcattca cagcttgcct cccggcatta agggaggtgt aaaatgcttt atgccaatga
      421 tctattttaa gaaaaataag ccgtccataa aagtcgcgat gggggcgacc ctaatgcgct
      481 ccttactgga agctggggtt ccggtggcct cctcttgtaa tgccgacggt gtttgcgcca
      541 aatgcggtat tcgcatcatc caggggatgg agaacctttc ccccgccaat cagaccgagc
      601 tttttttgaa agaaaaaaat aatatttcag tggatattcg tattagctgc cagactcagg
      661 tttggggaga tatcgttgtt gatgctgatt attggtgacc actcagcaag agacgccgca
      721 ttgtgatatg tctgcttttc cccttcgaat aatagtgtca atagtctctt aaattgataa
      781 tttcaagccc ttctaagagg tggcatgatg gatcaaaaaa ttgaaaatct tattattatt
      841 ggctcaggcc ctgccggact gacctcggca atttacagtg cacgtgctcg tctcgagcct
      901 ctgatgatcg aaggcgaaga agccggcggc caattgatga ttactacgga agttgaaaac
      961 tatccgggtt ttgagcacgg gattacagga cctgaactca ttgatgtgat gagaaaacaa
     1021 gccttgcgat ttggtacacg ttttgtttct cgcaatgtga ccaaagtgga tttctcacaa
     1081 cgtcctttca aggtttgggt gggggataag ctttatcttt ccaaggccat tattatttct
     1141 acaggagcta gtgctcagta cttgggtctt ccgtctgaaa agcaatattt gagccgcggg
     1201 gtttcggctt gtgcgacttg tgatggggct ttcttccgca atatggatgt cggagttgtg
     1261 ggtggcggtg atacggcgat ggaggaagca caattcctga ctcgctttgc aaaaaaagtt
     1321 tatgtaattc atcggcgaga tcatttccgc gcttctaaaa ttatgagcga aagaactctc
     1381 agtaatccaa aaattgaagt tttatggaac tccgaagtga gcgaagtttt aggtgatggt
     1441 aaaaatgtga ccgggttgag aatcaaagac acattgaaaa gctcgaccaa agacctggcg
     1501 gtagatgggg tcttcatcgc catcgggcat aaacccacaa cctccctatt caaaggtcta
     1561 ttggatatga atgaaacggg gtacttggtg accaaagcca attccacgta tacaaatatt
     1621 gaaggagtct ttgctgctgg cgatgtgcaa gatcatgtgt atcggcaagc gatcaccgca
     1681 gcggggactg gatgtatggc cgccatagac tgtgaaagat ggctggagac tcaagcccac
     1741 ggattaaaat aagaatatga aaagcaagaa aatcaatacg aaggacctca ttaataaaat
     1801 tgaaaaagtt gccaaggctc gtattgcgag tcagcacgtg gtgtctttag cagattttcg
     1861 cgaagccgtg aaaaaagaag agcctcgagt tattctggtc attgaagacg acgaaacaat
     1921 gcgaatggcg ctcaaaagaa tttttgaggc tgagggctat gttacgaaac ttgctgcgga
     1981 tgccacggct ctttcagagg ttttagatga tacgcccatc gatctgattt taatggatgt
     2041 aggcctccct tgggtgaatg gctttgagct ggctcaatta cttaaagaaa acaaagacct
     2101 taaggggatc ccactggttt ttgtatctgg taaggcgagt gaagaggata tgaagcaggc
     2161 tttcgaaatt ggcgctgatg actatataag aaagccattt gatgtggaaa agcttcgtaa
     2221 gacggtgcga acccttttaa aacttaatga gtagttacag gccaatttca ttggccaaat
     2281 ctagcccgca tcttttttaa ggcgattttg ctgagccata taatcgttga tcagcttact
     2341 gagtttcagg gttgtttctt catcttggtc tagaaaaacc agacgtcttg ggtcggcgat
     2401 atctccaatg attttacctt ttaagtgcac atgatttttt tttgtgtctt tttcaaactt
     2461 gttcaccaaa atcatttcaa aaggacgatg gaagaaatct ttcggaattt catcttctaa
     2521 gagagttccg gaaagagaaa tgtttcttga gtgggttcta aaagaacttc ctctttttgt
     2581 cagaagaacc gcctcaattt taaaatcatg gcgatgggca gtgcggcgat ctttaggagt
     2641 gttcaattcg ctacggctta cgacaatttc tacggaaggt ttatccggaa gtccagacag
     2701 tgtgaggtca tctcccttaa actcgttgta atagtaacca taatctgagg tttccaattt
     2761 ttccgcagcg accacagaag tataagaggg agcctccgaa gcgatccttt ggattttaac
     2821 ggtgacgtca gaggattttt gtttttcctt attaatcaaa ggttcaggag gttgggcttg
     2881 aacaaaatat ttttgcccac tcgaaaggaa ttgagtcaaa ggcaaccact ctgtccaacc
     2941 tggagtccaa atataatatt ggtggagctg tcgcatttta aaagtcagaa tcatcatttg
     3001 agtttgtaca acggacaatg gcttggtctt tttattcgca gccgaatcaa aaataatcca
     3061 aattttaccc tctgttttca tagtcccctc aatgaaaaaa accagacctt atgcttatcg
     3121 gcctggtgag gttgtttata aagggtaatg gtaaattaat ttaatgtgac taacttttga
     3181 tcagctttac taaacacgaa tacaagcagc cacatgatga ggacgttttt cctccagagg
     3241 gggcacggtt tttttacagt cttcaatggc catcgggcaa cgaggatgaa agtgacaacc
     3301 tgcaggagga tgaacgggac ttggaacgtc tccggtcaaa atgatgcgct cttctttgtg
     3361 ttttgggtct ggcacaggga tagcggataa caaagctcga gtataaggat gcttcggatg
     3421 cgtataaagc tcctcggatt cagacatctc gacgatttta cccagataca tcacagccac
     3481 ctgtgtagaa acgtgttcaa caacttttaa gtcatgggca ataaacacgt aggtcaatcc
     3541 tagcttcttt tggagctcca ttaaaagatt caacacttgg gcttgaatag aaacatccag
     3601 tgcagaaacg ggttcatcgc aaataataag ctcaggattt acggctaagg cacgagcgat
     3661 acctacacgc tgtctttgtc ccccagagaa ctcatgggga tagcggttca tggcttcagg
     3721 acggagtcct accaattgag ccaactccat cacacgttct ttgcgggctt tttctgaagg
     3781 gtaaaggttg tggatgatca aaggttcttc aaggattgaa ccaatggtca tacggggatt
     3841 taaacttgca taaggatctt gaaagatgat ctgcatcttt ctacgaatct cacgaagttc
     3901 gtcaccttcg atatgggtga tatcttgacc attgaaacga actgttccag aagtagggtc
     3961 caacaaacga atcaggcaac gacctaaggt ggactttccg cagccggatt ctcccacgag
     4021 gcccagggtc tccccttttt ttaatttaaa gttgacgcca gaaacagcct taacacttgc
     4081 aatttctcga tttaaaattc caccgttgat agggaaagtt ttcacaaggt tttcgacttc
     4141 gaggagatag tggctcataa tttgtctcct tgatcaggta caggatggaa acaagccgcc
     4201 agatgcgcgc cttgcttgct ttcaagtctt ggttcaactt gacggcagtc attttgaaca
     4261 tttggacagc gatcttggaa gcggcagccc ttaggaaggg ctaaaaggct tggcaccatc
     4321 ccttggatcg tttttaattg agttaatttg tgacctgttt caaagtgcgg aatagaatct
     4381 aaaagtcctc gagtgtaagg atgttttggg cgatagaaaa tatcttcaac agttccatat
     4441 tccacaactt ttccagcata catcaccacc acttcttggc acatttccgc aaccacgccc
     4501 agatcatggg tgattaaaat catgcccgcg ttgaaatctt tttgtagttt gcgcatgaga
     4561 tctaaaattt gagcttggat tgtaacgtcc aaagccgtgg tgggctcatc cgcaatcaaa
     4621 agttgaggat cgcagctaat cgccatggca atcatgactc tttggcgcat tccacctgaa
     4681 agctggtgag ggtattcgtt ataacgttgt tcaggggcag gaatacccac aatgcggagc
     4741 atttcaatgg cccgggcttt cacttcggct ttagtaaact gaggatagtg aagtgaaatg
     4801 gattcggaga tttgattgcc gcaggtataa acaggattca aagaagtcat gggttcttga
     4861 aaaatcatgg cgatttcatt gccccgaatg tggcgcattt cttcttccga agttttgagt
     4921 aagtccttgc catggtagag aatttcgcca gattcgattc tacctggaga ctggattaac
     4981 ttcataatgg agagcgaggt tacggatttt ccgcagccag attcgccaac aattccaatg
     5041 gtttgtccct ttttaacggt gaaactaaca tcatctacag cgtagaaggt gccgtcatca
     5101 gttttgaatt gtgtttttaa gctcttaact tctaaaaggg tttcattcac tgtctggttt
     5161 ctcccacttt aaagcccgtt tcttatacaa ggcctaaagc ctggcatcaa ccagataaat
     5221 caggtgctgc gaggtgaaat taaataaaca atatcaacga ctcatcaatt ccttgatgag
     5281 actatggagt gttgcatagc ttggcagagt cgctacggga ttatattttt gcaaaatttc
     5341 tatttttgaa tatccaaact ccactgcaat cgcgcccacg ccagcggctt gagcggcctt
     5401 catatcaggg atgccgtctc cgatcatgaa tgttgtggtc ttagaatgtt tcgccagctt
     5461 catcatttct tgcagaggca aggggtgagg cttacattca gagaaagtgt cggcgccaaa
     5521 gactccaacc caggagatct cctcatgggc cagtagattt aggtggtgga ggactttctt
     5581 ggcaggggct tcattcttat tggtcacaat tcctttaggg cctggatatt gccgcaagaa
     5641 ttccacagca ccaggataaa ggcgagtggt tttgtgcatc tcttcctcat aggtatttaa
     5701 gaaatgcaaa taaatatgct gatactcgtg cggcggactg tttttatgtt caggaaaaaa
     5761 gtcactcaaa agtttcagca ggccttctcc gatatgattt acaatcactt cgtgaggcaa
     5821 agtcgatttg ttatagaaag tgagagtttt attcagagct gaagagatat cctttgcgga
     5881 atcaatcagc gttccatcaa gatcaaaaat aagcagtggc caagtcatag aggcacctta
     5941 aagtatattt gcagtttagt caggacaatt tagtcagata atttgagtcc tcggttgtca
     6001 gttggaaaaa atttatgcta tctatgggca tgcgtatcga aatccctcaa gcccaagaat
     6061 atattgaatc acttgctgcc gtagaagctg cgagcaaaca aaaatcccgt cgcttcgcgg
     6121 aagaactcgg tctgggcaaa atctgtttgt cgccctctga agggcactta ttgtctttcc
     6181 tagtaagaat gcagcgatgt cggaagtttg tagagattgg aactctaacg ggactttcag
     6241 ctcaatatat tttagaaggc ctgggagagc agggcgaact ttggtctttg gagaaaacag
     6301 agcttcatgc ccagaaagcg ttggcggctc tcgctgagca tccggctgca aaaaatgttc
     6361 atattatcgt tggcgatgct cgagtgactt tggaaactct gaaagctcag ggaccttttg
     6421 acggcgtctt tattgatgga aataaggcgg cctatggtga ttacttggct tgggctgagg
     6481 ccaatgtttc ccccgggggc ttgatcatcg cggacaatgt ttttttaaat ggcgctgtct
     6541 ggggcgataa agaaggacaa agtcaaaaat tcagcgagaa gcaagtgaaa gtcatgcaga
     6601 ctttcaatca tcgcttaagt gaccctgtat tgtatgacag tgttcttctt cccaccaaag
     6661 aaggaatgca cttcgcggtg aagaagtttt aagaacgctt ttcggtctga cttaagtttt
     6721 tcaatattct tttcaagaaa tcaggatttt gtgaaagagc atgataggat tcctccctta
     6781 ctcataaata taatcaggcc tgtactgatc ggggtttttc caattgtgag gaatgttgaa
     6841 tatttttatc aactcctgcc aatcggtttc tgataggaga atatattcag atccaatct
//
LOCUS       DATBTE010000435         6736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2245906, whole genome shotgun sequence.
ACCESSION   DATBTE010000435 DATBTE010000000
VERSION     DATBTE010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2245906"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VIG33_16625"
     CDS             complement(<1..625)
                     /locus_tag="VIG33_16625"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY8272520.1"
                     /translation="MSAEETSKAVELLAKGQVVGMPTETVYGLAARIDLPEGIESIFK
                     TKERPFFDPLIVHVSDLAQLNEVVSSFPAVAVALAEKFWPGPLTLVLPKSSKLNPMIT
                     SGLDSVGVRMPAHAVAMDLIRKAGVPLAAPSANKFGKTSPTTANHVRQEFQKENIFVL
                     EGGDSKIGIESTVLLINETDGVNKLSILRKGHVLRSDIEKHLNSKNIG"
     gene            complement(635..3178)
                     /locus_tag="VIG33_16630"
     CDS             complement(635..3178)
                     /locus_tag="VIG33_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY8272521.1"
                     /translation="MWIRRELLRSWKFGLFFIFNLSLGLTGYISLEAFKVSLEDTLNQ
                     NSKAILSADLAVSIRRELTENEKTALQRVMAKDSEMSETYEFFAMLSSSKGSRLVMVR
                     AIDENYPLYGSLTLSSSKVGIQQKTPKKEIIGSNSAWIYPELEAQLGLRKGDSFQLGQ
                     LQLKVSDIVAKDETQTFRAASIAPRIFIDRSLLAQSGLIQFGSTYSVNYFFKLKNDQL
                     TEDRKTELLKVFKDPAIQVETAKTASEESSHQLAYLSDYLGLVAIIALFMSALGAAYL
                     FRLFMTERVREVAILRSLGLQAMRAVSLYAAQVIILGFLSLIPTVMVSSLLLPLLSEL
                     LSSLTPFDLHPVMSLKAVFMALILGVIGSFVICLPFLLKIQDLKPARLFAEEKFSTSI
                     DIHRPWVFLPGLILFWLLAVAESSSFKVGSLFVGAFFGVLLILYAVGMALLVVLKSAR
                     KRKNWIFKYSWLGLSRRRSSSLAIFIALGLGSLLINVLPQLKVGLQNEFKTDAVSKVP
                     SLFLFDIQDEQLAPVQDFLQQEKFESIGFSPMIRARILKINDQPYERKSEERGFKTRE
                     EEMEARFRNRGINISYREKISASEKIVEGRNFSGNYDASKQKYPEISIEAKYAGRLRM
                     KVGDILQFDVQGVQIEGQVINLRKVKWTSFQPNFFILVQNGVLNEAPKTFITALPKMN
                     EAKKEELQLQLAKKFPNISIVDVQRTVNEVLKISDQMSWSLELMAALALFTGYVVLYS
                     IVSGQVNLRRWELNMLKVVGAKFSAISNYLVIEFVTLSFCAGLIGASLSMVVSYVISY
                     FIFEGSFIFNWIWPILTALGVCLLSGLIAFFASRRVVRESPLVILQEER"
     gene            complement(3188..3853)
                     /locus_tag="VIG33_16635"
     CDS             complement(3188..3853)
                     /locus_tag="VIG33_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8272522.1"
                     /translation="MSLLIKNLEKSFNQGEDKLTVLAGLNLDIKNGERVAIVGQSGSG
                     KSTLLSLLAGLDQADSGEVVVDGVNISAMSEEQITEFRGQNIGIIFQQYHLVAHLTSL
                     ENVMLPMEILGRPDAEAQAQALLKEMGLEHRMNQFPSRMSGGECQRVAIARALAVRPK
                     ILLADEPSGNLDTHTGEKVMDIFFNAAKKYQITTILVTHSEELAKRCQKTLRLCEGQL
                     LEV"
     gene            complement(3850..4449)
                     /locus_tag="VIG33_16640"
     CDS             complement(3850..4449)
                     /locus_tag="VIG33_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY8272523.1"
                     /translation="MRLFLFLILLFSIAFAVTNKKLVVIGDSLTEGLGVSKESAYPAL
                     VEKKIHDSGKKNWTVINAGVSGSTTASVVGRMKWLFKSKPDLILLVMGANDGLRGLKI
                     EESEKNLSEAIEYTQSQKVPLVLGGIYIPPNYGKDYTEKFKKMYLTLAKKYKIPLIPF
                     VLDKVGGDPRYNQADGIHPNEAGHKIIAETVYQQIKGLL"
     gene            complement(4524..4883)
                     /locus_tag="VIG33_16645"
     CDS             complement(4524..4883)
                     /locus_tag="VIG33_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEY8272524.1"
                     /translation="MGKKLYVGNLPYSVDDESLHQRFSEFGAVSSAKVITDRETGRSK
                     GFGFVEMDSDSDAEAAMDKLNGTQMDGRAINVSEAKPQAPREGGGRGGFGGGGRGGGG
                     GGRGGFGGGRGGGGGRY"
     gene            complement(5010..5492)
                     /locus_tag="VIG33_16650"
     CDS             complement(5010..5492)
                     /locus_tag="VIG33_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY8272525.1"
                     /translation="MASFDIVSEINIQEVDNAVNQTRKEVEGRYDFKGSQAEIQWDKK
                     EIIFLAEDDYKIEAMGGILQSKLHRRGVDIKSFKFEKPEPAGGKLLRQKITITQGIEK
                     EVAKDIVKFIKDSKLKVQPQIMEDKVRVTAKSIDELQETIALVRSGNFPVPLQFNNMR
                     "
     gene            complement(5510..5986)
                     /locus_tag="VIG33_16655"
     CDS             complement(5510..5986)
                     /locus_tag="VIG33_16655"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEY8272526.1"
                     /translation="MKRRVFAFNYVLKGVDGQTLDASDAGEPLAFLEGTGQIIPKLEE
                     EIITMKAGDKKVVKLAAKDSYGEPDPKMTMEVPKEELAHLKIELGAFLQLNLGEQTKV
                     VRVANIGEKVVTLDGNHPLAGVDLVFDIDMVTTREATAEELQHGHAHGATGHGHHH"
     gene            6116..6655
                     /locus_tag="VIG33_16660"
     CDS             6116..6655
                     /locus_tag="VIG33_16660"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY8272527.1"
                     /translation="MHHLSSLKSLLLNSSYEPMRIVSWQKALILWFQDKVEILEYHAM
                     FARSMRASYQMPSVLRLKSYVKPRAAGAVRFCRENVYIRDNYTCQYCGHHLTPKNLTL
                     DHVVPASKNGKKSWTNVVSACRQCNQRKANRTPHTANMPLLSEPKMPSWLPSTELKFK
                     IEIEKAPASWLEYLRFKAG"
BASE COUNT         1930 a         1627 c         1317 g         1862 t
ORIGIN      
        1 atccaatgtt ttttgaattt aaatgctttt ctatatccga ccttaaaaca tgaccttttc
       61 ttaagataga aagtttatta actccgtcag tttcattgat cagcagcact gtggattcaa
      121 taccgatttt actatcgcca ccttcaagga caaaaatatt ttctttttga aattcctgac
      181 gaacgtgatt ggccgtcgtc ggacttgtct ttccaaactt attcgcactg ggggcggcca
      241 gggggactcc agccttgcga attaaatcca tagccaccgc atgtgccggc atacgcacgc
      301 ctacggaatc caaacccgag gtaatcatgg gattcagctt actactttta ggaagaacca
      361 gggtcagggg gcctggccaa aatttctcag ccaaggcaac ggccaccgcc ggaaagctgg
      421 aaaccacttc attaagctgt gccaaatccg atacgtgaac aatcaaggga tcgaaaaaag
      481 gccgctcctt ggttttaaaa atggactcaa tcccctcagg caaatcaata cgcgcggcta
      541 aaccatagac ggtttcagtg ggcattccta caacttgtcc ctttgccaaa agctcgacgg
      601 cttttgaagt ttcttctgcg ctcatagaag ggttttaacg ctcttcctgc agaatcacaa
      661 gaggactttc gcgaaccact cgacggctgg caaaaaaagc gatcaggccg cttagtaagc
      721 aaacacccaa ggcagttaga atcggccaaa tccagttaaa aataaaactg ccttcgaaaa
      781 tgaagtagga aataacatag ctcaccacca tgctaaggct cgcgccgata aggcccgcgc
      841 agaaagacag ggtcacgaat tcgatgacca gatagttgga aatagcacta aatttagctc
      901 ccacgacctt aagcatattg agttcccagc gacggagatt aacttggccg ctgacaatgg
      961 aatacaaaac cacatagccc gtaaaaagag ccaaggccgc cattaactcc aaagaccaac
     1021 tcatttgatc cgagatcttc aaaacttcat tcactgttcg ttgcacatcc acgatagaaa
     1081 tgttggggaa tttcttagca agctggagct gcaattcttc tttctttgcc tcattcatct
     1141 ttggcaaggc cgtaataaat gtctttggcg cctcattgag aacgccgttt tgtaccaata
     1201 taaaaaaatt gggctggaaa ctggtccatt taactttacg caaattaata acctgtcctt
     1261 ctatctgtac cccttgaaca tcaaattgta gaatatcacc aaccttcatg cgaaggcgtc
     1321 ccgcgtactt agcctcaatt gaaatttctg gatatttttg tttagaggca tcataatttc
     1381 ctgaaaaatt tcttccctcg acaatcttct cagaagccga aatcttttca cgataagaga
     1441 tatttatccc ccggttgcgg aagcgtgcct ccatctcctc ttcacgtgtt ttaaagcccc
     1501 tctcttcact ctttctttcg taaggctgat catttatttt taaaatgcgt gcacgaatca
     1561 tcggagaaaa gccgatgctc tcaaacttct cctgttgcaa aaaatcctgt acaggcgcca
     1621 gttgttcatc ctgaatatca aacaagaaga ggcttggaac cttcgatacc gcatctgtct
     1681 taaattcgtt ctgcagacct acttttaatt gcggtaaaac attgattaat aaagaaccta
     1741 atcccaaagc gataaaaatc gccagactgc tcgagcgcct tcgagacaaa cccagccagg
     1801 aatatttaaa aatccaattt tttcttttgc gcgcggattt caaaaccact aacaaagcca
     1861 ttccaaccgc atacaaaatc agtagcactc caaagaaagc tcctacaaaa agcgaaccta
     1921 ctttgaagga actggactcc gccactgcca gcaaccaaaa aagtatcaat cccggtaaaa
     1981 aaacccaagg ccgatggatg tcgatggagg tgctaaattt ttcttcggca aagaggcgtg
     2041 ctggttttaa atcttgaatt tttaaaagaa aaggcaagca aattacaaag ctgccgatca
     2101 caccgagaat gagagccata aacacagctt tcaatgacat gaccggatgc aaatcaaagg
     2161 gagtcaaact tgataaaagt tcacttaaca gcggtaacag caaagaggat accatcactg
     2221 tggggatgag agataaaaag cctaaaatga ttacttgggc ggcatacaaa ctcaccgcgc
     2281 gcatcgcctg cagtccaagg cttcgcagaa tagccacttc tcgtactctc tcggtcatga
     2341 aaagacgaaa taaatacgcc gctcccagtg ctgacataaa aagtgcaatt atcgccacta
     2401 gtcccagata atctgacaaa taagcaagtt ggtgactgga ttcttcactc gccgtttttg
     2461 ccgtttcgac ttgaatggcc ggatctttga atacctttaa aagttctgtt tttcgatcct
     2521 cggttaattg atcatttttt aatttgaaaa aataattaac agaataggta cttccaaact
     2581 ggatcaggcc agattgcgcg agcaaagagc gatcgataaa aatccgtggt gcgatggagg
     2641 ccgcacgaaa agtttgggtc tcgtcttttg ccactatatc agaaaccttc aactgcagct
     2701 gacctaattg aaaagagtcc cctttacgca gtccaagttg agcctcaagt tccggatata
     2761 tccaggctga gttggagcca ataatttctt tttttggagt tttctgctga atcccaactt
     2821 tggaagagct cagagtcaaa gatccgtaga gagggtagtt ctcatcgatg gctcgcacca
     2881 tcactaaacg agaaccctta cttgagctta acatcgcaaa gaactcgtag gtctcactca
     2941 tttcggaatc tttagccatc accctttgta gtgccgtttt ttcgttttca gtgagttcac
     3001 ggcgtataga cacagcaaga tccgctgaaa gtatggcttt agaattctga ttgagagtat
     3061 cttccagaga aactttaaaa gcttctaaag agatataccc ggtcagcccc aagcttaggt
     3121 taaaaataaa aaaaagccca aacttccaac ttcttaaaag ttcccggcga atccacatga
     3181 ggaacactta aacctccaaa agttgacctt cgcaaagtct taaggttttc tggcaacgct
     3241 tcgccagttc ctcactatga gtgactagaa ttgtggtgat ttgatatttt ttagcggcgt
     3301 taaaaaagat atccataact ttttcgcccg tgtgcgtgtc cagatttcca ctgggctcat
     3361 cggcaagtaa aatttttgga cgcacagcca gggctcgcgc gatggcgact cgttgacatt
     3421 cgccgccact catccgactg gggaattgat tcatgcgatg ttcgaggccc atttccttca
     3481 gcaaggcctg agcctgcgcc tctgcgtctg gacgtcctaa gatctccatc ggcaacatca
     3541 cattttctaa ggacgttaaa tgggcaacca aatgatactg ttgaaaaatg attccaatat
     3601 tttgaccacg aaactctgta atctgttctt cgctcatggc actgatattc actccatcta
     3661 caactacttc accgctgtcg gcttgatcaa ggcccgcgag caaagagagt aaagtggatt
     3721 taccgcttcc tgattggccg acaatagcca ccctctctcc gttttttatg tcaagattca
     3781 accccgccag gacagtgagt ttgtcctctc cttgattaaa ggacttttca aggttcttaa
     3841 ttaacaaact cataataaac ctttaatttg ctgataaact gtttcagcaa taattttatg
     3901 accggcttca ttgggatgaa taccatcagc ttgattgtac cttggatctc cgcccacttt
     3961 atcgaggaca aaaggaatca atggtatttt atattttttc gccaaagtca ggtacatttt
     4021 tttaaacttt tcagtgtagt ccttgccata attaggagga atgtaaattc cccctaagac
     4081 aagggggact ttttgtgatt gagtatactc aatggcctca gaaagatttt tttcgctctc
     4141 ttcaatttta agtccacgca aaccatcatt ggcacccata actaataaga taaggtcggg
     4201 tttagattta aaaagccatt tcattcgtcc tacaacggat gctgtggtgg aaccactcac
     4261 ccccgcatta atcacagtcc aatttttttt accgctgtca tggatctttt tttctaccag
     4321 cgcagggtaa gcgctctcct tagaaactcc gagcccttca gtgagggaat cgccgatcac
     4381 cacgagcttt ttattagtca cggcaaaagc aatggaaaat aaaagtatta aaaataaaaa
     4441 aagtctcatg cttccatgag actctttttt agacttaact gacaagtccg agcgtataat
     4501 attctgccta atgacagctt agactagtaa cgtcccccac cgccgccacg tccaccgccg
     4561 aagccgccgc gtccgccacc gccgccgcca cgtccaccac cgccgaaacc gccacgtccg
     4621 ccgccttcac ggggagcttg tggcttagct tcagatacgt tgattgcacg gccatccatt
     4681 tgagtgccgt tcaatttatc catagctgct tccgcatcag aatcagaatc catttctacg
     4741 aatccgaaac ctttgctgcg gcctgtttca cgatcagtga taacttttgc agaagaaact
     4801 gcgccgaatt cagaaaatct ttggtgaaga ctttcgtcgt cgactgaata tggaagattg
     4861 ccaacatata attttttacc catgggtacc tccagggatc tttagaccgc ggaagccatg
     4921 agcaaaacaa aactctagga acatcgaacg cggaacgtta ataactcaca ttaatttatc
     4981 acagcttatt cttcacgcaa gtgaaattcc taccgcatat tattgaattg caatggaacc
     5041 gggaaattcc cagaccggac caaagcaata gtctcttgca attcgtcgat actttttgcg
     5101 gttacgcgaa ctttatcttc cataatctgt ggctggactt ttagcttaga atctttaata
     5161 aattttacga tatctttagc cacttctttt tcgataccct gcgtgatggt gatcttctgc
     5221 cgaagcaatt taccaccagc gggttctggt ttctcaaatt taaacgattt gatatccacc
     5281 ccccggcgat gcagttttga ttggaggatc ccccccatgg cctcgatctt ataatcatct
     5341 tccgctaaga aaataatttc tttcttatcc cattgtattt ccgcctgact gcccttgaag
     5401 tcataacggc cttccacctc tttacgagtt tggttaactg cattgtctac ttcttgaata
     5461 tttatttctg atacaatatc gaacgaagcc atgagttcta aactttctct tagtgatgat
     5521 gaccgtgacc ggtggcaccg tgggcatgac catgttgaag ttcttccgcg gtcgcttcac
     5581 gggtggtaac catatcgata tcaaaaacta aatccacccc cgctaaagga tgattgccat
     5641 ccagggtaac tactttttca ccgatattgg cgactcgaac cacttttgtt tgttcaccta
     5701 aatttaattg caaaaatgca cccaattcaa ttttcaaatg agccaattct tctttgggaa
     5761 cttccatagt catttttgga tctggttctc cgtaagaatc cttggcggca agctttacga
     5821 cttttttatc tcctgctttc atagtgataa tttcttcttc caatttagga ataatttggc
     5881 ccgtgccttc caggaaagct aatggctcac cagcatccga agcatcgagg gtctgaccat
     5941 cgactccttt taggacatag ttaaatgcaa aaactcgacg tttcatttta agtctccttt
     6001 gaattaatag ggagctatcg cgaaacaacc ctaaattcaa gggattatgg tccaggtact
     6061 tgaaaatccc cctataaacc cgcataattt aagaagagaa atcgatggag gtgtaatgca
     6121 tcatttatcc tcgctcaagt ctctgcttct caattcgagc tatgaaccga tgcgaattgt
     6181 gagctggcag aaagccttga ttctttggtt tcaggataag gtcgaaatcc tggaatacca
     6241 tgcgatgttt gctcgttcca tgagggccag ttatcaaatg cccagcgtgc tgaggctaaa
     6301 aagctatgtc aaaccgcgag ccgctggcgc cgttcgattt tgccgcgaaa atgtctatat
     6361 ccgcgataat tacacctgcc aatactgtgg tcaccatctc acccctaaga atctcacctt
     6421 ggaccatgtc gtccccgctt caaagaatgg aaagaaaagt tggaccaatg ttgtgtcggc
     6481 ctgtcgtcaa tgcaatcaac gcaaagccaa ccgcactcct cacactgcaa atatgccttt
     6541 attaagtgaa cctaaaatgc catcatggct gccaagtact gagctaaaat ttaaaataga
     6601 aatagaaaaa gccccggcca gttggttgga gtatttacga tttaaggcag gatgagggta
     6661 agatcgaatt ggacctcata aagtcatgta caagtatttg attcctcgct agattctatc
     6721 tatcgaggaa ttatga
//
LOCUS       DATBTE010000436         1935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_743447, whole genome shotgun sequence.
ACCESSION   DATBTE010000436 DATBTE010000000
VERSION     DATBTE010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743447"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..522)
                     /locus_tag="VIG33_16665"
     CDS             complement(121..522)
                     /locus_tag="VIG33_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8272528.1"
                     /translation="MRQIDFGFLQKQSFSFGGSSLLGKRKTARPLSTKKPLHLVLKSQ
                     GSSLFNPGNRDLEKLIKDHAKKYSIAVYAFSFNWSHIHLVIKLTTRESYRAFIRTVTA
                     AIVSFLSKQKGRTLKGTFTLRPFTRIFTAVR"
     gene            complement(1668..>1935)
                     /locus_tag="VIG33_16670"
     CDS             complement(1668..>1935)
                     /locus_tag="VIG33_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272529.1"
                     /translation="QSNVLKNLMTVAETKIIEMKLALDKKQSEAADYYSHLQKALAQV
                     NVLNQENLILKDYVNKLNYYHQQAQAVQAAGIQAQQPTTPTPAK"
BASE COUNT          546 a          347 c          449 g          593 t
ORIGIN      
        1 ttttacgaga aatacgtgtt ggcgtattga gacaaagcat tgccctgagc aatcctcccg
       61 atggttgccg ctggaaacaa cccctagatc atcgtttgac gcctatgaaa atttaatttt
      121 ttaccggaca gcagtgaaga ttcgagtaaa agggcgaagg gtaaaagttc ccttaagagt
      181 tcttcccttt tgttttgaaa gaaaagaaac aatggctgcg gtgacggttc tgataaaagc
      241 gcgatagctt tctcgagtgg taagtttaat aactaaatga atatggctcc agttgaagga
      301 aaaagcataa acagcaatgg aatacttttt agcatgatct ttgattaatt tttccaggtc
      361 tcggttgcca ggattaaata aggatgaacc ctgactttta agcaccagat gcagaggttt
      421 tttcgtagaa aggggccggg ctgtttttct ttttcccaag agggaactcc caccgaagct
      481 gaagctttgt ttttgcaaaa aaccaaaatc aatttgtctc aaaggaaacc tccgacagag
      541 gttatagcat gacttttccg aagccctttt aggggagagc tgaggtgtta tttggcttcc
      601 tgtttaggta acagaaggcc ttcgccctgg agagatcttg ggctgagttg aagtttttgc
      661 cgtcaaggaa agaaaaactg tttatattca attcttgagc ggagaaggag ccgtcaactt
      721 attttttctt ggataagaaa tttttcagac gctattcttg atggggatta aagctcaggg
      781 attgttccga ggaagaggaa ggcctggttg cataagtcgc gaagacgctt gtcgaataaa
      841 aagacttggg gcgcgacttc acttccgatt gcgtacaatc agaagtgcgg taaaagtggc
      901 ggtatagaaa gtagcaatta tgattacgaa tgtaatttaa agagttttga ggtttgccct
      961 caaatggatg tctgatgtca aatcaaatta cccccccccc tatccccact ttttctagaa
     1021 agaggcttga atctgtatag agtttagaca atcgaaaatt ctttatagat ttcaaaagaa
     1081 aatttcaatt aaatacgcat tctagttatc ttccgatttc ctaaatattt gagctaataa
     1141 atttaaacaa ttacatttta ggagtttgtg tgaagttact ttttatagta ctctcgattt
     1201 ttataggttt aagttctatg gcatcgccaa gagatagatt tggtgcccgc catggatatg
     1261 gaccaagata tgaaagatcg ttggattatg tcggggcact taacctttta actcaagagt
     1321 gcgaaagaat gtccagagtg gactacatag ccgaatacta ccgtcgcggt ataagggcta
     1381 tcttatcaac tagctccact tcaccttacg agaattctgg ccaatcacca tatttatggt
     1441 ccgaggtgaa tatgtacggt aaatattctg agggtggtgg ctgcaacatg tacgggtgtt
     1501 atgacaacgg aggtaattgt aatatgtacg gttgctacca agctggcggt caatgcaata
     1561 tgtatggatg caccagagaa gctgataagt cccaacgaac gtgttttgaa cagtagattg
     1621 cttccaattg aaagaaagcg agatcggatt tttcctctct cgcactttta tttagcgggg
     1681 gttggggtgg tcggctgttg cgcttggatt ccagcggctt gcacggcctg ggcttgttga
     1741 tgatagtaat tcaatttatt gacgtaatct ttcaaaatta agttttcttg gtttaaaaca
     1801 ttgacctgag cgagagcctt ttgcaaatga ctgtaataat cagcggcttc gctttgtttt
     1861 ttatccaggg ccaacttcat ctcgatgatt tttgtttcag ccacggtcat gaggtttttt
     1921 aagacattgc tttgc
//
LOCUS       DATBTE010000437         1844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_879995, whole genome shotgun sequence.
ACCESSION   DATBTE010000437 DATBTE010000000
VERSION     DATBTE010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879995"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1485
                     /locus_tag="VIG33_16675"
     CDS             175..1485
                     /locus_tag="VIG33_16675"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8272530.1"
                     /translation="MFARKSLEILKKWYASKKRKPLVLRGARQVGKTYLVNQLAQDLK
                     INLIEINFELQTFTSLNAETINMKSLINEIELNIGRRLQPKNDLIFFDEIQKSPRAFV
                     ALRYFYEKHPELAVVAAGSLLDFLFQDQTISIPVGRIEFHFLGPVTFSEYLLAKNKML
                     LYEEYLKKPEKISPGAHQLLRQEWTSFLITGGMPECVQGDADNEDIFQIRKIQKNIFN
                     TYKADILKYSKGTQITRCESVFDYVPGHIGQKVKYSEIDPDERSKNLKAAILLLTYAR
                     IVLPVFHSNATSIPLKSVADERIFKLFHLDVGLVSAGLQINSPLVPSSPSFGAMSEQF
                     VAQHLAYLDSSDEPQLFYWLKDKSSVKAEVDFIHSHNGQVVPIEVKSTRSAKSRSLSV
                     ITEVLKGIKTTIKYSPDEYRLKNSEGIRQIECPIYLVERINEFI"
BASE COUNT          580 a          313 c          377 g          574 t
ORIGIN      
        1 gaagttgtta gttatgagtt tgtggtgaag cctacggctt attgatttga tagcctttct
       61 attggtccca atatatctat aaatctatct tttgtcatta aaatgccatt tttatgattg
      121 aaaatagcat ttttatgtta aaaaataggc ttatagactc atggagtttt ttagatgttt
      181 gctagaaaaa gcttggaaat cttaaaaaaa tggtatgcct ccaaaaagcg gaaacctctt
      241 gtcttaagag gagctagaca ggttggtaaa acttatttgg taaaccaact cgcacaggat
      301 ttgaaaataa atctcataga aattaatttc gaacttcaga ctttcacgag tttgaatgct
      361 gaaacaatta acatgaagag tctgatcaac gaaattgaat taaatatcgg ccgtcgtctg
      421 caaccgaaaa atgatttaat tttttttgac gaaattcaaa aaagccctcg agctttcgtt
      481 gctctgcgat atttttatga aaaacatccc gaactagcag tggtagcggc tggttccctt
      541 cttgattttt tgtttcagga ccaaacaata tctatacctg ttggacgaat tgagtttcac
      601 tttctagggc ctgtaacctt tagtgaatac ctgttggcaa aaaacaaaat gctcctctat
      661 gaagagtatt taaaaaagcc agaaaaaatt tctcctgggg cccatcaact attgaggcag
      721 gaatggacgt cattcttaat aacaggtggt atgcctgagt gtgttcaagg cgatgccgat
      781 aacgaagata tttttcagat tagaaaaatt caaaaaaata tattcaatac ctacaaggct
      841 gatattctaa aatattctaa aggaactcaa ataaccaggt gtgaatcagt ttttgattat
      901 gtccctggcc atattgggca aaaggtaaag tattcagaaa ttgatcctga tgaaagatct
      961 aaaaacctca aggcagcgat cttgttactg acctacgcgc gaattgtatt gcctgtcttt
     1021 cattcgaatg cgacctctat tcctttaaaa tcagttgccg atgaaagaat atttaaactt
     1081 tttcatctcg atgtagggct agtaagtgcc ggacttcaaa ttaactctcc actggtgccc
     1141 tcatcaccta gttttggcgc catgagtgag caatttgtag cccagcattt agcttacctg
     1201 gattcttcgg atgaacctca attattttat tggctcaaag ataaatcctc tgtgaaggct
     1261 gaggtcgact ttattcacag tcataatggt caagttgtac ccatcgaggt taaaagcact
     1321 cgctcagcca agagtcgctc tctaagtgtt atcactgaag tgcttaaggg cattaagacg
     1381 actataaaat attctcctga tgagtatcga ttaaagaatt cagaaggcat tcggcaaatc
     1441 gaatgcccta tttacctcgt cgaacggatt aatgaattta tctagattct ccactagctt
     1501 tctttttgca tgatcttcct aagaacaatt gtcaattgaa agttgtattt agggtgcttc
     1561 catcttgggg tgagagggaa aaaagttatt tgtattgaat tcttgagagg agaggtagct
     1621 cgtcaactta ttttttcttg gagaagaatt ttcttgagag ggattaaagc ttcagaggat
     1681 ctttcgatga agagaatcca ggaatgaatt aagggaaaga ggaaaagaaa tgtctggttt
     1741 gcataagtcg cgaaggattt tggaatacga agagaaaatg ggggcgcgac tttacttctg
     1801 attgcgtaca atcagaagtc cggtaaaagt atgggagaaa gtat
//
LOCUS       DATBTE010000438         2823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_743602, whole genome shotgun sequence.
ACCESSION   DATBTE010000438 DATBTE010000000
VERSION     DATBTE010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743602"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VIG33_16680"
     CDS             <1..223
                     /locus_tag="VIG33_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEY8272531.1"
                     /translation="PFQAEDFLHRIGRTARAGRSGNAITFVTPSDHRMYNKIKTYLQG
                     AEEVKVDPQFKFIDRSLKFAKAKSKNKKR"
     gene            complement(240..1109)
                     /locus_tag="VIG33_16685"
     CDS             complement(240..1109)
                     /locus_tag="VIG33_16685"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY8272532.1"
                     /translation="MNITLDQVRCFCALVDCGSYQAAAKEMNKSHSAIVYSIGCFERQ
                     TGLTLLNRLEYRTTLTAAGERVYHQCRTLLSEATQLEQLCSDLSEGWEPSVRLVYDGV
                     LSPEPFLQIFKYFKDEQIPTLLDIYSDYLYGVEKSFTELEAQFMISILPAKQSGLQSL
                     ELNPISNILVAHRDHELFKQKQPWSLHELKAFNFFTVRGADKSLNLGTKELEENSTFH
                     LSEFSLKKVAILQGAGFGWLPQYLIQDELDLEIIKPIQWEGSSFQELHPRLYYKKKNK
                     IGRASQIILGKLR"
     gene            1212..2342
                     /locus_tag="VIG33_16690"
     CDS             1212..2342
                     /locus_tag="VIG33_16690"
                     /inference="COORDINATES: protein motif:HMM:NF042789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEY8272533.1"
                     /translation="MIPYIKGQVTTQAHVDVPEGTFEEEYARNGFSGRYAHLYRSEPP
                     VQWISIEGPLRPQAYDLNQVPRLAEDDYLLSRTPYLENKDLRILFSKLTQAMKYYFRN
                     ADGDEVLFMHKGLGTLETDFGPLQYEPGDYIIIPRGTVYRLVPVEPTELLIIESFSEV
                     RIPDKGMLGQHALFDPAVLKTPEPVRNPPKNPGKRFELKIQRCGQLTSVIYPFCPLNT
                     VGWKGTLTVWQLNVRDIRPILSDRYHLPPSAHSTFVANNFVICTFLPRPLENGDPKAM
                     KVPFYHSNIDFDEVLFYHQGQFFSRAGIQAGMMTFHPQGIHHGPQSQAVARTVGVRET
                     NEIAIMIDTKNPLIATSKAESIELKEYWKSWMDQTGVSKDGK"
     gene            2332..>2823
                     /locus_tag="VIG33_16695"
     CDS             2332..>2823
                     /locus_tag="VIG33_16695"
                     /inference="COORDINATES: protein motif:HMM:NF026047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272534.1"
                     /translation="MANDIGLDGIDFVEFVSPQPEKLERLFKEFGFSKLMRLQDRQVD
                     YFNQNDIHFFLNREAGTHAAQFGQLHGPAICSMGWRVKDAKHAYELAMSRGAKPALKS
                     DFPQWPAIYGIGDSLIYFIEKQSDLQRYEKVGFKKLAHPEMVPPKGFSAIDHLTNNVE
                     KGTM"
BASE COUNT          785 a          579 c          643 g          816 t
ORIGIN      
        1 gcccttccag gctgaggatt ttctccatcg tattggaaga actgcgcggg ctggtcgttc
       61 tggaaatgcg attacctttg taaccccttc cgatcatcgt atgtacaata agattaaaac
      121 ctatttgcag ggtgctgaag aagtgaaggt tgatcctcaa ttcaaattta tcgatcgctc
      181 acttaagttc gctaaagcta aaagtaaaaa taagaaaagg tgaaatttgt tcgctctatt
      241 catcgcagtt tgcctaaaat aatttggctg gcccgtccga ttttgttttt ctttttataa
      301 tagagccggg gatgcagttc ttggaaactg gatccctccc actgaatagg tttgattatt
      361 tcgagatcga gttcgtcctg aattaaatat tggggaagcc aaccaaaacc tgccccttgt
      421 aagatagcca cttttttcag agagaactca ctcagatgaa aggttgagtt ttcttcaagt
      481 tctttggtcc ctaaattgag gcttttatca gctccgcgaa cggtaaaaaa attaaaagct
      541 ttaagctcat gcaaagacca aggctgcttt tgtttgaaga gttcatgatc tcggtgtgcc
      601 actaaaatat ttgatatcgg gttgagttcc agggattgaa gtcctgattg cttggcgggc
      661 aagatggaaa tcatgaactg cgcctccagt tctgtgaaac tcttttcaac tccataaaga
      721 taatctgaat atatatcaag aagtgtgggg atctgctcat ctttaaaata tttaaagatt
      781 tggagaaagg gttcagggga taagaccccg tcataaacta gacgtacgga gggctcccat
      841 ccttcagaaa gatcggagca gagctgttca agttgagtgg cttcgctcag caaggttcga
      901 cactgatggt acacacgctc tcctgcggca gtgagggtgg ttctatattc caggcgattt
      961 aacagagtca gaccggtttg acgttcaaaa catccgatcg aatagacgat cgcggaatgg
     1021 cttttgttca tttccttggc ggcggcttga tagctgccac agtccacaag tgcgcaaaaa
     1081 catcggacct gatccagggt tatattcata agcacttgat atcattaaat tatagattgt
     1141 caaatatctt aacaaagaga gtgataactt tgttctattt tttataatca gaagcttgaa
     1201 tactctttcc catgattcca tatattaaag gtcaagtgac cacacaagct catgttgatg
     1261 ttccggaggg aacttttgaa gaagaatacg ctcgcaacgg attcagtggg cgctatgctc
     1321 atctttatcg ttcagagcct ccggttcagt ggatctctat tgagggtcct ttgcggccgc
     1381 aagcctatga tctgaaccaa gtgccgaggc tggctgaaga tgactacttg ctgagccgca
     1441 cgccttattt agaaaacaag gatttacgca ttctattttc aaaattgacg caggcgatga
     1501 aatattattt tcgtaatgcc gatggcgatg aagtcctctt tatgcataaa ggtctgggaa
     1561 ctctcgaaac ggattttgga ccccttcaat atgagcccgg ggattatatc attattcccc
     1621 gtggaaccgt gtacagactt gtacctgttg agccgacaga actgctcatt attgaaagct
     1681 ttagtgaagt gaggattcct gataaaggaa tgttgggaca gcatgctctt tttgatcctg
     1741 ccgtccttaa gactccggag ccggtccgca atcccccaaa aaatcccggc aaacgctttg
     1801 aattaaagat tcagcgctgt ggccagttga cctcggtgat ctatcccttt tgccccctta
     1861 atacggtagg atggaaaggg actttgacgg tttggcaatt gaatgtgcga gatattcgac
     1921 ccattctttc ggatcgttat catttaccgc catccgcgca ttctactttt gtggccaata
     1981 attttgtgat ttgcacattt ttgcctcggc ctcttgaaaa tggcgatcct aaagcgatga
     2041 aagttccttt ctatcattca aatatcgatt ttgatgaggt tctcttttat caccaggggc
     2101 aatttttcag ccgcgccgga atccaagcag ggatgatgac ttttcatcct caggggatcc
     2161 atcatggacc gcagagtcaa gcggttgcgc gcacggtggg agtacgggaa accaatgaaa
     2221 tcgccataat gattgataca aaaaatccat tgatcgctac gtccaaagct gaaagtattg
     2281 agctcaaaga atactggaaa agttggatgg atcaaacagg agtgtctaaa gatggcaaat
     2341 gatattggtt tagatggaat agattttgtg gaatttgtgt ctccgcaacc agaaaagctc
     2401 gaaagacttt ttaaagaatt cggtttttca aaattgatgc gattgcaaga tcgccaagtg
     2461 gattatttca atcaaaatga tattcacttt tttctcaatc gggaagcggg tactcatgcc
     2521 gctcaattcg gtcaactcca tggaccagct atttgttcca tgggctggcg agtaaaagat
     2581 gcgaaacatg cttatgaact ggcaatgtcc cgaggagcga agcctgcact gaaaagcgat
     2641 tttccgcaat ggccggcgat ctatggtatc ggtgacagct taatttattt tattgaaaaa
     2701 caatctgatt tacagcgcta tgaaaaagtc ggttttaaga agttagctca tccggaaatg
     2761 gttcctccta agggattctc agctatagat cacctgacta ataatgtgga aaaaggcacc
     2821 atg
//
LOCUS       DATBTE010000439         3659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1356632, whole genome shotgun sequence.
ACCESSION   DATBTE010000439 DATBTE010000000
VERSION     DATBTE010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356632"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VIG33_16700"
     CDS             <1..189
                     /locus_tag="VIG33_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEY8272535.1"
                     /translation="LGKPLKINWLEMPENVRGQYQYYTEAKTEKWLKAGMSPAKWPLE
                     KAVDDYVKNYLTKSDPWL"
     gene            200..1939
                     /locus_tag="VIG33_16705"
     CDS             200..1939
                     /locus_tag="VIG33_16705"
                     /EC_number="1.14.16.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0009072 - aromatic amino acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid hydroxylase"
                     /protein_id="HEY8272536.1"
                     /translation="MEALPKHLRKYVVEQNYAKYTPVEQASWRYILRQLKAFLSGHAH
                     ETYLEGLEKTGIEIESIPHIENISAKLQKFGWRAIPVSGFIPPAAFMELQSLGVLPVA
                     SDMRTLDHLLYTPAPDIVHEAAGHAPILANREFADYLRQYAQISRKALISKEDLNLYE
                     AIRELSDIKENPVSLPEQIKASEDKLERVSKSIKYISEAAELGRMNWWTAEYGLIGDL
                     KNPKIYGAGLLSSVGEAKWCLSEQVKKIPLTVDCIKQGYDITEPQPQLFVTPDFKALV
                     KVLNEMADQMAFRKGGMTSVQKALRAESVNTIQFNSGIQISGVVTEALTPPHPSQEVA
                     YLCFQGPCQLSYNDQELGGHGTTYHAQGFGTPVGKLKKFPSLCPSELSDSQWDSLNAA
                     PGKKLELEFTSGVRVTGTFNGRVEQEGKTILLSLTGAKAIYQDRVLFDPSWGTFDMAI
                     GSSIPSVFGGAADREAFGETDDFVAARVPQRTYSDEEKFFQSQYARVREIREKKIQDT
                     ELEKELCKILENHDQRFKTDWLLRLEAYELVSQRARNSNLKRKLQADLERIASLDSKV
                     SSMIQDGLAHAQL"
     gene            1926..2717
                     /locus_tag="VIG33_16710"
     CDS             1926..2717
                     /locus_tag="VIG33_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY8272537.1"
                     /translation="MRNFEVRIVLVRTIYERNIGATSRAMSNMGFENLILVDPQCEIT
                     YEAQQTAATGQTGLQNRTTYKSWAEFLEKEPESIKICFTARDGKGRQVRDIDEVLSDV
                     KEKSPQFQQQEENPHPIPYLVHLIFGPEDWGLSGEDLEIANFCACIPTFGDNWSLNLA
                     QATLLAMFSLRKAWGGQRTPLDGGDLRRAPQGIEGINPDLTLKKWLLEMGFDLSKKKM
                     NVYTVLRRMLLQNTPTKKEWVILETVLQQSIRKLQEYNELRKNLK"
     gene            2886..3431
                     /locus_tag="VIG33_16715"
     CDS             2886..3431
                     /locus_tag="VIG33_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272538.1"
                     /translation="MKSKFSSLKKNLNSILAMVSILTFGLVLFGPSWSNPFVFDDIIK
                     IQENSDLKTEVSILKTLIYPYQKNTTHLRNDPSRPLVYIIYRLCFQAANGQPWPFHLV
                     NTIIHCLNAVLVFLFAGLLTGRLFLNSSLKPGTVSAFLFLVLPINANSVLYAYALSEL
                     LVTFFLLAALYAYFKSEKFVS"
BASE COUNT         1024 a          827 c          782 g         1026 t
ORIGIN      
        1 ctgggtaagc ccttaaaaat aaattggctt gaaatgcctg aaaatgtccg tggccaatat
       61 caatactaca ctgaagcaaa aactgaaaaa tggttaaagg caggtatgag cccggccaaa
      121 tggcctttag aaaaagccgt ggatgattat gtgaaaaact atctcaccaa gtctgatcct
      181 tggttataaa ggatgagaaa tggaagcact tcccaagcac ctccgcaaat acgtcgttga
      241 acaaaattac gctaagtaca cccctgtaga gcaggcctct tggcgctata ttttgcgtca
      301 actgaaagcc tttttatctg ggcatgccca tgaaacttat cttgagggcc tggaaaaaac
      361 cggaattgaa attgagagta ttcctcatat tgaaaacatc agtgcgaaac tgcaaaaatt
      421 tggctggcgg gccattcctg tcagtggttt tattccacct gcggctttta tggagcttca
      481 gtcgttggga gtgctccccg tcgcctctga catgcgcacg ttggaccatt tactgtacac
      541 tccggctcca gacatcgtcc acgaagctgc aggtcatgct ccgatcttag caaaccggga
      601 gtttgcggat tacctacgcc aatacgctca gatttcccgt aaggccctta tcagcaaaga
      661 agatcttaat ttatatgaag ccattcgcga gctttcagac atcaaagaaa atccggtttc
      721 acttcctgag caaataaaag cctctgaaga caaacttgaa cgcgtttcta aaagtataaa
      781 atacatctct gaagcggctg aacttggacg catgaattgg tggacggctg aatatggcct
      841 cattggtgac ttaaaaaatc ccaagattta tggggccggc ctgctttcca gcgtcggcga
      901 agccaaatgg tgtcttagtg agcaggtgaa aaaaatacct ctcactgtgg attgcatcaa
      961 gcaaggttat gatatcaccg aacctcaacc acaattattt gtaactcctg attttaaagc
     1021 tctcgtaaaa gtcctcaatg aaatggctga tcaaatggcc ttccgcaaag ggggaatgac
     1081 cagtgtgcaa aaggcccttc gcgcggagtc tgtgaatacc attcaattca attctggaat
     1141 tcagatttcc ggggtagtga ccgaagcgct gactccgcca cacccttcgc aagaagtcgc
     1201 ttatctctgt ttccaaggtc cttgtcagct gtcttataac gaccaagagc ttggtggcca
     1261 tggaaccacc taccatgccc agggttttgg aactcccgtg ggaaagctca aaaaatttcc
     1321 tagcctttgc ccttcggaat tgtctgatag ccaatgggat tcactcaatg cagcccccgg
     1381 taaaaagcta gaacttgaat tcacaagtgg agtccgcgtt actggaacct tcaatggccg
     1441 tgtggaacaa gaaggtaaaa ccatcctttt gtcattgact ggagcaaagg ccatttacca
     1501 ggaccgtgtt ctcttcgatc cttcgtgggg aacttttgac atggccattg gctcttcgat
     1561 accttcagtc tttggcggcg ccgcagatcg cgaagccttt ggtgaaactg atgacttcgt
     1621 cgcggcccgg gttccacaga ggacttattc agatgaagaa aaattcttcc aatcccaata
     1681 cgcccgggtt cgcgaaatcc gcgaaaaaaa gatccaggat acagaactcg aaaaagaact
     1741 ttgtaaaatt ttagaaaacc atgatcagag atttaaaaca gattggctgc taagacttga
     1801 agcctatgag ctggttagcc aacgggccag gaattcaaat ctgaaacgga aattgcaggc
     1861 agacctggaa aggatcgcca gcctggattc caaagtctcc tcaatgattc aagatggttt
     1921 ggctcatgcg caactttgaa gtgcgtattg tcctcgttcg cacgatttat gagcgcaata
     1981 ttggcgccac ttcacgggcc atgagtaata tgggatttga gaacttaatc ctcgtcgatc
     2041 cccagtgcga aatcacttat gaggcccagc aaactgcggc cacaggccaa actggcttgc
     2101 aaaaccgcac gacctataag tcatgggccg agtttttaga aaaagaacct gaaagtatca
     2161 aaatttgttt taccgctcga gatggcaaag gtcgccaagt ccgtgacatc gatgaggttc
     2221 tttctgatgt caaagaaaag tctccccaat tccaacaaca agaggaaaat ccccatccga
     2281 ttccctatct tgtccatttg atttttggcc ctgaagattg gggtttaagc ggtgaagacc
     2341 tggaaatcgc aaacttttgc gcgtgtattc ccacctttgg tgataattgg agtttaaacc
     2401 tagcgcaggc cacactgctg gcaatgttct cgctgcgtaa agcttggggc ggacaacgca
     2461 ctcccctgga tggcggagat ctcaggcgtg cgcctcaagg tattgagggc atcaatcccg
     2521 atctgactct taaaaaatgg cttttagaaa tgggctttga tctgtccaaa aagaaaatga
     2581 atgtttatac ggttttacgc agaatgctat tgcagaatac ccccacaaaa aaagaatggg
     2641 tgattcttga aactgttctg cagcaaagta tccgcaaact gcaagagtac aatgaactcc
     2701 gtaaaaatct taagtaggga atggccagtt cattggcaac caaaacgtga tcttgtcact
     2761 cctaactact ggtaatattc tgtgacttcg atgtagcctg ccgcatctgt agatccagca
     2821 aagccagaag tacctgcggt tattcagcag ttgaagattt gagaaattca ttcaaactaa
     2881 gtaatatgaa gtcaaaattt agttctttga aaaagaacct caattctatt ctagcaatgg
     2941 tttcaatttt aactttcggt ttggtacttt ttggcccgag ctggtcgaat ccttttgtat
     3001 ttgatgacat tattaagatt caagagaatt cagacctcaa gactgaagta agcatattaa
     3061 aaacacttat ttatccctat cagaaaaata cgactcatct tcggaacgat ccatcccgtc
     3121 ccctcgttta tataatttat cgtctttgct tccaggcagc caatggccag ccttggcctt
     3181 tccatctggt aaatacaata attcattgtc ttaacgctgt cttggttttt ctttttgctg
     3241 ggcttttaac aggccgacta tttttaaatt cttcattgaa gcccggcaca gtatcggcct
     3301 ttcttttctt ggtacttcct atcaatgcca attcggttct ttatgcatat gccttgtcgg
     3361 agcttctagt aacatttttt ctacttgccg ctctttatgc ttattttaag agtgaaaaat
     3421 ttgtaagcta aatttcattt tttcaatttt tttcttttta tgtgctcttt tttcaaaaca
     3481 aagtgctgtc atatttccgg gtcttttgat tgctaccgat ttgctctttg gaaatgaggt
     3541 cgcaaaacgt cgctttcatt atttaacctt tatcggtatc acaatcatat atatactcat
     3601 tcgctacact attcttggag gcatcggcga tttagaggct gagggacaga cctttttag
//
LOCUS       DATBTE010000440         1942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2452291, whole genome shotgun sequence.
ACCESSION   DATBTE010000440 DATBTE010000000
VERSION     DATBTE010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2452291"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VIG33_16720"
     CDS             complement(<1..590)
                     /locus_tag="VIG33_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272539.1"
                     /translation="MKKQVMTLMCVITSVCSVVVQASTSLLPGTTVIVPGTTLGTEVD
                     LAGGVVINKLVPFKIVTSSGKLLFRGNLQNRIVRSASDGNLHFYYAIRDTEAGLPGKI
                     LALTTESFRTTPVISTNYRVDGFGMVAPQTASRSYNGAQVMFRFSHRGPGMFDAGENS
                     KFFVIKTEAKHYNEFGYTRITLRSGQSVLLKTAQPL"
     gene            complement(769..1428)
                     /locus_tag="VIG33_16725"
     CDS             complement(769..1428)
                     /locus_tag="VIG33_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY8272540.1"
                     /translation="MSISIREIKKSFGFNATPILKGISAEIETGEMVSITGRSGSGKS
                     TLLYIISSLDRPTSGDVLIDDKSLLLMNSKDLHQFRNQKMGFVFQFHHLLPELTAVEN
                     VLMPALKTKSAEQHRQFAMELLEEFGLKDRMNHLPSQLSGGEQQRVAVARALVMQPTY
                     LFADEPTGNLDSVNGLLVMGLFKRINKEQGTTIIYVTHDQEFARMSSHQINLVDGSLM
                     S"
     gene            complement(1428..>1942)
                     /locus_tag="VIG33_16730"
     CDS             complement(1428..>1942)
                     /locus_tag="VIG33_16730"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY8272541.1"
                     /translation="RDLASTLNLMGQEKVQSWDQSNEGIMSIFTTQDIVRNSMTLSIL
                     VVAGFGIYNILSLAVTHKRREIAILRSMGFEPYDISALFLTQGLILGLIGGVVGCFLG
                     LTASYGMSLIRVSEARGLGTGQLMISFNYMIYVKAFILALASSSFASYFPARSAGLLE
                     PIDIIRSENS"
BASE COUNT          576 a          435 c          395 g          536 t
ORIGIN      
        1 attaaaggtt gagctgtttt caggagaaca gattgtccgg aacgcagggt gattctcgta
       61 tacccaaatt cgttatagtg tttggcctct gttttaataa caaagaattt tgaattttcc
      121 ccggcatcaa acattccagg gcctcgatga gaaaatcgga acatcacttg agctccatta
      181 taagagcgag aggctgtttg aggagctacc ataccgaacc catcaactct atagttcgtg
      241 gaaatcaccg gggtcgtccg gaaactttct gtggtgagtg ctaaaatttt tccaggcaaa
      301 ccggcctcag tgtcgcgaat ggcataatag aaatgtagat tgccatcact cgcagagcgc
      361 acaatccgat tctgtaagtt tcctcgaaaa agaagcttcc ctgagcttgt tacaattttg
      421 aagggaacaa gcttattaat cacaactcca ccggcaagat ccacctccgt tcctaatgta
      481 gtcccaggga ctataaccgt tgtgcccgga agaaggctcg tagaggcttg cacgactaca
      541 ctgcaaactg acgttatgac gcacataaga gtcatgactt gttttttcat tttcaatacc
      601 tcatccttgg ttggtttggt tttgtttggc atctgtaaac ccaaaaaaat cctaaagcaa
      661 taagatggtt tctttaaata atttgaacaa agctatgtca aagatctcaa ctttttatct
      721 ttatcgtttt ggttatgacc aaaagagggg gaaaaatgaa taggaacttc aactcatcag
      781 ggaaccatca accaaattaa tttggtgcga agacatacgg gcaaattctt ggtcatgggt
      841 cacgtaaata attgtcgttc cctgctcttt attaatgcgt ttaaataaac ccatgaccag
      901 gaggccattt actgaatcaa gatttccagt gggttcatcg gcaaaaagat aagtgggctg
      961 catcaccaat gcccgagcca ccgccaccct ttgctgttca cctccagaga gctgacttgg
     1021 caaatgattc attctatcct tcagcccaaa ctcttcgaga agttccatcg cgaattgtcg
     1081 atgctgttca gccgatttcg ttttcaaagc aggcattaaa acattttcta cggctgttaa
     1141 ctccggaaga agatggtgaa attgaaaaac aaatcccatt ttttgattac gaaattgatg
     1201 gaggtcttta ctgttcatta gcaataaaga tttatcatca ataagaacat cacctgaagt
     1261 ggggcgatct aaactgctga taatatacaa aagtgtgctt ttcccggaac cagaacgccc
     1321 agtaatggaa accatttccc ccgtttcgat ctccgcagag attcctttta aaatgggtgt
     1381 ggcattgaag ccgaaacttt ttttaatctc tcgtatagaa atagacatca cgagttctcg
     1441 cttcgaataa tgtcaatggg ctctaaaagt cccgctgacc ttgccggaaa atagctggca
     1501 aagctcgagg aggccaaagc cagaataaag gctttaacgt aaatcatata attaaatgag
     1561 atcatcagtt gtcctgttcc taatcctcga gcttctgaca ctcggatcag cgacattcca
     1621 taggaggccg tcagtcctaa aaaacatcct accaccccgc ctattaaacc tagaattaat
     1681 ccttgagtca ggaacaaggc gctgatgtcg taaggctcga aacccatgga acgcagaatg
     1741 gcaatttccc gccgcttatg agtcactgct aagctcaaga tattataaat accaaatccc
     1801 gccaccacta atatcgaaag cgtcatcgaa tttctaacta tgtcctgggt ggtgaatata
     1861 gacatgatac cttcattgga ttgatcccaa ctttgaactt tttcttggcc catcagattc
     1921 agggtagatg cgagatctcg gg
//
LOCUS       DATBTE010000441         1640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2041286, whole genome shotgun sequence.
ACCESSION   DATBTE010000441 DATBTE010000000
VERSION     DATBTE010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2041286"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1395
                     /locus_tag="VIG33_16735"
     CDS             241..1395
                     /locus_tag="VIG33_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272542.1"
                     /translation="MNRDSVKGSYYIRVSKSGWYVCYQFYKDGVRSQASVAKLAYREL
                     GFKTEMSVDEAKERCKLLNAERGLIKDKIRVAAKRVTEIKNINETLFPTHSVEAFQEL
                     LKEENFGSEAHLEKLYSHFNFIQTMVNYLRLQPFEYKDNSKKIYKYFISKQISLNYSY
                     RLLTVLNRWGKFHSKTTGQFYDDVKAPRGREQSAIADAQKTKSGKETELGVRTESLPL
                     VPEKLQAAKDKLIPDQFNWLKISVWFGLRPEEVDLLDDDTQFRVEFNLKSKTKVLHVY
                     QPKLQSIPENKRWKRIPVVFKEQEECLELIAEGNFERPLNKTIRKHCGQGITCYGGRK
                     NFVDMMLDKGQRIEDISLWLGHTSIETTWKHYKNRQTINFIETAETSVSK"
BASE COUNT          516 a          354 c          327 g          443 t
ORIGIN      
        1 agtaaagttt tgccagctcg tcctggtcga tgcttggttt tttccactga acccgataca
       61 ctctctttac agagaactgg gttggttcga gaatgtttgc atggccccta ccaaattttt
      121 gaaaaagcca agtaatttta aatacttggc tttttttatt gtcggcggtt tgtgtaggct
      181 gtgtaaccat ttctattcgc aattttcaca cttaaattag agatacgata tacacccttt
      241 atgaacagag attcagttaa gggttcttac tacattcgag tttcaaaaag cggctggtac
      301 gtgtgctacc agttctataa agacggcgtg cgttcgcagg cgtccgttgc aaaactagca
      361 taccgcgagc ttggctttaa gacagagatg tctgtcgacg aggccaagga acgctgtaag
      421 ttgctaaacg ccgagcgcgg cctaatcaaa gataagatca gggtcgccgc caagcgggtc
      481 actgaaataa aaaacattaa cgagacgcta ttccctactc attccgttga agccttccag
      541 gaacttttaa aagaagaaaa ctttggttcc gaagctcatc ttgaaaagct ctattctcat
      601 ttcaacttta ttcaaacaat ggtcaattac cttcgattgc agcctttcga gtacaaagac
      661 aattcaaaga aaatctacaa gtacttcata tcaaaacaaa tcagtctgaa ttattcttac
      721 cgcctactca ccgttttaaa ccgttggggt aagtttcata gtaagactac tggccaattc
      781 tatgacgacg ttaaagcacc aagaggccgc gagcagtctg ccatcgccga cgcccaaaag
      841 actaagtccg gcaaagaaac cgaactcggg gttcgcaccg agtctcttcc tttggttcca
      901 gaaaaattgc aagccgcgaa agataagtta atacctgacc aatttaattg gttaaagatt
      961 tccgtatggt ttggtctaag acccgaagaa gtagaccttc tagacgatga cactcagttc
     1021 agagtcgaat tcaatctcaa atcaaaaacc aaagttctgc acgtctatca accaaagctt
     1081 caaagcatcc cggaaaacaa acgttggaag cgaattccag tcgtcttcaa agaacaagag
     1141 gaatgccttg agctaatagc tgaaggcaac tttgaacggc ctttgaataa aacgattcgc
     1201 aagcactgcg gccaaggtat aacctgctat ggcggtcgta aaaactttgt agacatgatg
     1261 ctggataaag gccaacgcat tgaggacatc tctctatggc ttggacatac ctctatcgag
     1321 acaacctgga aacactataa aaatcgtcag acaatcaatt ttatcgaaac agctgaaaca
     1381 agtgtctcaa aataaatcca agaatttaca gcatggaaaa agctgttttt tagtctatca
     1441 aagactattt ttttgagaaa aaaagaatct aaacctagaa aaaaatcaat gttgccttga
     1501 ttatgagcac tcgccttcat ccaagcctcg caaatacgcg tgcagtgcgg cttttattaa
     1561 aaaaatttgt aataactacc ttagttgctc ttgttttatt catgtggata ggtatccttt
     1621 aatcggctgg agaggatagc
//
LOCUS       DATBTE010000442        24540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_402068, whole genome shotgun sequence.
ACCESSION   DATBTE010000442 DATBTE010000000
VERSION     DATBTE010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 24540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24540
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402068"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VIG33_16740"
     CDS             complement(<1..623)
                     /locus_tag="VIG33_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272543.1"
                     /translation="MTKKLLNTPWFYCALSFVHQCLVILPGEFPKLFPDSGSYLSWYP
                     HRTPLYPFLLSVFQTTENTAIFQMLIFALSAGAFHLICRMVIETLQETLQKKFPSAKA
                     KEIFIWTGSLYFATNFELLQFAPTILTESLGISFFTFYFFSVLRWSSLRENAGGAASF
                     LVKLYFVLSFLFFPIVLFMLRPSFVLVPAAIYFFHVLKFLQQKKYTL"
     gene            complement(623..1186)
                     /locus_tag="VIG33_16745"
     CDS             complement(623..1186)
                     /locus_tag="VIG33_16745"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEY8272544.1"
                     /translation="MTKRFFDTILALSLLGIFLAPLLIISLVVKWTSPGPVLHWSKRV
                     GKDNKLFLMPKFRSMKINTPQVATHLLTNAQDHLTPVGSFIRKTSLDELPQLWSVLVG
                     DMSFVGPRPALFNQDDLIKLRTERGVSGLRPGITGWAQINGRDELPIPIKVEFDHYYF
                     KNASLLLDLKIIFRTAYKVFNGEGVKH"
     gene            1330..3189
                     /locus_tag="VIG33_16750"
     CDS             1330..3189
                     /locus_tag="VIG33_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEY8272545.1"
                     /translation="MFLRIAELPRKFKILIMLGCDVVLLPLALWSAVALRLGTFTPDV
                     SQFWWVFFVIPFTAVPIFIRFGFYRAVIRYMDDKIIYTVIYGVSLSVLMLMAVVVMAK
                     LVALPRSSIVIYWVIAIAYIGASRFVARGLIRTIETRRDRKQNVAIYGAGQAGLQTAL
                     ALVSGREYRPVAFFDDNPELQGTSVAGLRVYPPKEALKIMQQKGCEQLLIAMPSSSRT
                     VRKEIISRFEGKDIVLKILPGLGDLVQGKVRVEDIREVGVEDLLGRDPVPPFEQLISS
                     CIKGRVVLVTGAGGSIGSELCRQILLNSPLKLILFERSEFALYKIEQELTRLHPEAQV
                     ISVLGDVLDQEHLENLMTAHAVQTVYHAAAYKHVPLVEANADSGILNNVFGTRAAAKA
                     AINTKVSTFVLISTDKAVRPTNVMGASKRLAELVLQGLAHEPQQHTRICMVRFGNVLG
                     SSGSVVPLFKEQIKNGGPVTVTHPEVTRYFMTIPEAAQLVLQAGSMGKGGDVFVLDMG
                     EPVKIVDLARKMVELSGLEVREPHSENGDIAIEFVGLRPGEKLYEELLIGDNVTWTEH
                     PRIMRAEEHFLEENFLKEKLTEIQAACKQGDSIKIRGILKSLVKEYTLETFNR"
     gene            3197..3910
                     /locus_tag="VIG33_16755"
     CDS             3197..3910
                     /locus_tag="VIG33_16755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY8272546.1"
                     /translation="MIVLIVPCYNEEKRLDLEAFEEGAQGEIEILFVDDGSKDNTVSR
                     IEKFIAGKPNLHLYSCAKNGGKAAAVREGMLYAIQHPALSQANWMGYWDADLATPLWE
                     VPNMIKYAELYDTKVDSIWGSRVYRLGSKIVRSPHRHYLGRGFATVISLMLKVESYDS
                     QCGAKLIRREHIQTAFGESFLSNWIFDVEIMLRLKGKLLVEYPLRQWEDIPGSKVKIF
                     REILRVFQDILKIRKKYLG"
     gene            complement(3920..5335)
                     /locus_tag="VIG33_16760"
     CDS             complement(3920..5335)
                     /locus_tag="VIG33_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272547.1"
                     /translation="MGRKYRELKEALRTNPTRSLLVIDDWGLRLKLFSEAESIIKKID
                     KVERDRDNFSNIDQRLFNDWHDLTFRQEKKEMEDRRQEYISLAKFHNDIIAVAEMQNI
                     PPHRAYHFLKEEEQRYQAGTETQRAKIDSLRQERSEFAQKAMDEEFGSPFDFEDDDEG
                     ESQDDFEALNKDALNKIDTKLYHHLKSTDPDHLRTEFSGPEAHFLAMNAMRIASLSED
                     PSLLIKIWDILSLKTQRHLSKDFKKNTGTPMEKFIEELRELFNGQPFEESSTNEDSDD
                     NDFIRTSPGRSFSLSDNEERDLKFVFRKLVRLIHPDSQDPKLNENLKAWYNKVWHRVQ
                     EAYKNKDLHQLRRLEIMATIRLKELNNLTFGEIKASTKWLEEELAELRASLKSYEKHP
                     AWKFSTKRTYDALIKRIQKEFQMNLMPILKDIEQIKDIHNFYEKASNPNLRASAKKRK
                     SQKRPFRKPKKKSSSQMSLFD"
     gene            complement(5432..5896)
                     /locus_tag="VIG33_16765"
     CDS             complement(5432..5896)
                     /locus_tag="VIG33_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272548.1"
                     /translation="MPERWRIFCLALNEIQLKTDFAAHAFVLMGNHFHVLFSTRSQKE
                     HILAEEFHQTLTTLCNKTWEALEEPLFCDPILSAEYYKNAYKYVYRNPLEAGLCRTVE
                     EYEYSSLRSLLGYSKLDAPVIDNMGLIHRPIKILDWLNHESIEFNTAFITKV"
     gene            6131..6256
                     /locus_tag="VIG33_16770"
     CDS             6131..6256
                     /locus_tag="VIG33_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272549.1"
                     /translation="MNEMNAMQKNLLILNSQAKALGTVELFLRNAKRCGIGGGKI"
     gene            6628..7422
                     /locus_tag="VIG33_16775"
     CDS             6628..7422
                     /locus_tag="VIG33_16775"
                     /inference="COORDINATES: protein motif:HMM:NF003842.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY8272550.1"
                     /translation="MPNSVKKTKNNSNFFTAPFYVPSASDSLISVGAFSEKTLPEKDL
                     KVLIWNVYKGKDPRWEKEFRDIIRGHDLVLLQEAVTDHRMPKIWRDDFAQYAWNMAAS
                     FHLNPQEATGVVIGAMAESFDLKFVRTKSRELFILTPKIALLSKFKISGRNEKLMVVN
                     THSLNFTTTSLFIKSVEEKVQQIESHKGPLIFAGDFNTWNTRRWILLVQILSELKLYP
                     VDFANDTRFFKLDHVFVRGIDIHQNQVLHGFKGSDHTPLEVVMSFE"
     gene            complement(7447..9615)
                     /locus_tag="VIG33_16780"
     CDS             complement(7447..9615)
                     /locus_tag="VIG33_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272551.1"
                     /translation="MVLEIDCRNYFNAKCEKIPPGLKRSLEACEINAKAHNCEKIAGE
                     HPEIKDLLMSCKPQDFCHQQIMSDVGEALSCWKGNINSVMDTLKALNAPIEAAWQKLK
                     STPLKDYHLPSLPSLPDLRNVHFDPEKTKRKLVDLWHVAETKSAEKYHHYNCYTPEAK
                     EELQCYFFATLVDPTVIAGKSVALLKARRLLKALDQESDMAQDIGNAGIAATKALSKE
                     QFTAKYLHYSPTDKNQNLEWMGQAEKSTVSKNIHFFDVENSELKTLNDTVKDKNWVTS
                     LTNYHKEILHTKTKKLLDELKWEYPDLEISAYSDFKASRFAFKGGAPPDLNERLNKIL
                     NETNDEFTQYVTKNKFIDPSTHPENWFRGGFGGTADQANLASRFSRQSAENTLHSYSS
                     AEMKSAMTTKLKAVESQRASLQNELGKTSMVEGSDKKVLTSDALDIIRKNGDDIQKSQ
                     ADLKNRFGLEKLSTSTVQKMHAYAKSVDEFAPGIHIAERQVANLDEAVFGGFSADMAG
                     MGAKNLSGTASALSNAKDLDEALVQTRKAEKIVTEQFAKQKQVFQDVVKEVLGPERIK
                     TVCSGDDCIAIPKQILKDGEKKKILQRLSDAGYSSQFRLSFIPDNVTEVAARNQLSVH
                     GESIEKILRKKLADKMEPMKLKGVIFGVDMKTTELNKGSIQLITANSSEVKLTPSEQR
                     LIMNNFEKAVEQINEDLAQQGKASTYFALKPELNHNNMKP"
     gene            complement(9831..10331)
                     /locus_tag="VIG33_16785"
     CDS             complement(9831..10331)
                     /locus_tag="VIG33_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272552.1"
                     /translation="MKDFESFLESGQRQMNPSVLENIKKEIFPAPSRVALKMGLIHLA
                     SSLLTLMACPQFGLRLFFEGHGLMHYFMKAGSVVCFAFCGAFYLGASFWIAKIFLGEF
                     EWEVLRKNPTLSLGALSLLSLGIFSMMSHDLHFEAGFVWLLGAILSGAIPLLTRVHHR
                     PLIKFP"
     gene            complement(10344..10880)
                     /locus_tag="VIG33_16790"
     CDS             complement(10344..10880)
                     /locus_tag="VIG33_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY8272553.1"
                     /translation="MINKSDQELLLSLAQGNNKALDYLFKRHSNKVYNYALKRKFNTE
                     QAQDLVQIVFMQIYRKRIQYDPHYAALAWIYVITKSELKDYKLRELKNFAEFNESVSQ
                     NLGHIPNREYNGFEDNQEVQYYISGLDEKEKQILQDRYLNELEYEEIAQKLNESESNI
                     RQIVSRSLKFLRKKHATK"
     gene            10903..11544
                     /locus_tag="VIG33_16795"
     CDS             10903..11544
                     /locus_tag="VIG33_16795"
                     /inference="COORDINATES: protein motif:HMM:NF017833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEY8272554.1"
                     /translation="MPKTQQEHEIANSRLLKAIEMIEKETEHKGLTLGELIELLGEMG
                     HAVLILLLCLCFLQPIPLPGISTPIGIVIMISATLQFLNLPPWIPRQFRQHMISQKVL
                     HKTAVVARKIWSKIERFLNPRLLFLTKTPTFRFINLVLIIISAFLLALPLPIPFTNTV
                     PSIVILSIVFAQLEEDGFSILFAYFMTFVMSIFFYSLGAGIWTFAQRPWASLL"
     gene            11671..12978
                     /locus_tag="VIG33_16800"
     CDS             11671..12978
                     /locus_tag="VIG33_16800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8272555.1"
                     /translation="MKFVIFGKSVSSVEQSLKLSNLDAFLYSLMVGAGESYLPAYALS
                     IGMGEVFAGILASLPLVSGAVLQLFTPRLLHKVHSHKYWVVLSTLLQAMAFLPLIYFS
                     LTRAPNFWVLFFIFTLYWGAGFSAGSAWTFWMGHLVPEKESAKYFSFRARIQQIGIVL
                     GIVGGGVALHNKVEIGPFSSVFSLLFIFAFVCRLTSTLVLSKKMYRSEWVLNDQVHRL
                     RDSWSLFWAGQHKKKFFAYLIPFQVAIYMSSPFVTPYMLAQLKMNYGQYMGAIAALMI
                     GKIISLSFMQKAKSNFDGFKVMMLGLVLISPLPALWALNEAFYFVVGLQILSGMGWAC
                     FEVGLALVFFQDLRQEEKVPVLTIYNLLNSVAIIVGTFLGARFLHMMGEVKNSYWILF
                     VVGASLRAVFCVPVVLQSRRWKKISDQQEAAAAPEELLISKVY"
     gene            complement(12958..13740)
                     /gene="tsaA"
                     /locus_tag="VIG33_16805"
     CDS             complement(12958..13740)
                     /gene="tsaA"
                     /locus_tag="VIG33_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEY8272556.1"
                     /translation="MNSSVSFTPIGIYHSDAKEPYEAARQPLERVTTQGEITLRSGQQ
                     FEQALENLEGIERVWLLYIFHHNIHWKPKVLPPRGSTTKVGLFATRAPYRPNPIGLSC
                     VKLLKVSDLRLLVEGADLLDGTPILDIKPYLPYADSHPSSKTGWLENPRRYAVLSSIA
                     ARLQVGFLRERGLSQLQDFILNQLEYDPINRDKKRVSAVQDHFVLAYKTWRVQFTVDE
                     EKLEVTVVKLFSGYSEADLAPAAADPYLDKNIHREFLVHFGN"
     gene            complement(13737..14537)
                     /locus_tag="VIG33_16810"
     CDS             complement(13737..14537)
                     /locus_tag="VIG33_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEY8272557.1"
                     /translation="MHPKDLKALQNIHRQMRELEQRAAAQGFFSLETLSKLQKDLEAF
                     ITHPDLEFRKISEIAPHLHLEMTMKHYVSWIVPVERLLQKKLRDDEFLVFSQDGAHKA
                     QEKIPLVFILHNLRSAFNVGSIFRTAECFGASKIYLCGYTPLPTQDQLAKTAMGTDTL
                     VEWQEIPKIADLLLRLRRNNNYNIVALETASHALPLENVFPDMPTAFVLGNERFGIDP
                     EILQLCTEVRKIPLRGQKNSLNVGVAAAIAAYEFSRQYTSQYNGWSSL"
     gene            complement(14555..16630)
                     /gene="metG"
                     /locus_tag="VIG33_16815"
     CDS             complement(14555..16630)
                     /gene="metG"
                     /locus_tag="VIG33_16815"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY8272558.1"
                     /translation="MNTLSISPRPLPRKILMTCALPYANGYIHIGHLVEYLQADYWNR
                     FQNMRGHQCIFICADDTHGTPIMVKAREQGITPEQLIAKSYEEHTTDFKDFQIEFAHY
                     GSTNSVENRVLIEQFYKKFQDGHHIVSRPIKQLYCEHDKMFLPDRFVKGTCPSCGSKE
                     QYGDSCDVCGATYAPADLKDVHCSICGNTPVLKDSESIFFKLNDYKKYLEEWIPQHCA
                     SDIGKKMLEWFNEDLHDLDISRDEPYFGFAIPGTNNKKFFYVWVDAPMGYMSSTEQWA
                     KKQSQTLADIWQDPTREIYHFIGKDIARFHTIFWPAFLKAAEFKSPDQVFVHGHLMVN
                     GDKMSKSKGTFIAARTYLNHLNPEFLRYYYATKLNGTAEDIDLNLEDFVTRVNSDLVG
                     KITNLASRGAQMLKKKMDGVMTAPDKQGEELIRYAQAKNEEIAKHYEGRDFVKATNEI
                     RALADKANQYFDEKAPWKTLESNPQSTKEVLTTTLNIFRILSIYLKPILPHYTARVEK
                     LFGEKPYSWVDAQKILLGTGIQEYEHLASRIDADKVKAMVEEAKALNEKIASEKQSAT
                     ASASGIKIKNRSEIKSAEIEFDDFMKIDLRIGTVVEAEEIKEADKLLRLKVDIGGGEL
                     RQIIAGIKAAYSPDKLLGRQVLVCVNLKPRKMKFGMSEGMVLAAGEGGSDLYVLSPDS
                     GAKTGQRVK"
     gene            complement(16658..17521)
                     /locus_tag="VIG33_16820"
     CDS             complement(16658..17521)
                     /locus_tag="VIG33_16820"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEY8272559.1"
                     /translation="MKILVMILAGLSLVNCSSKPAAETSSAHAPSAQSPVPPHLSAGT
                     EPLKKQADKELEELKVPKKNSEITTPAVVSAAKPKTVPSKKHSVPRGPVASEKALGWL
                     KNGNIRFVKHSLRNDGDTAKDRARVASSPNPHAIIISCSDSQVPPEIVFDQKLGEIFV
                     IRTAGESLDTAAVASIEYGLEHFGSNLIVVLGHESCEAVKTALATLGGEDAGSPSMNR
                     LMANLRPHLKSFEGKPLSEGTLIEGWANAEGVAKDLIGRSQIVRDALNTGEVKIQTAL
                     YHLGSGVVEFK"
     gene            17602..18549
                     /locus_tag="VIG33_16825"
     CDS             17602..18549
                     /locus_tag="VIG33_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY8272560.1"
                     /translation="MSVPLLSSDRILKKLALYSYEQKKTYLAMYSSWYGGITKDPALM
                     MVPVDDHLVHRGDGVFEAMRFLHGKVYLMNDHLDRLESSAKQLGMEWPFGREKMVQII
                     EEVVKVAGADELMLRLFLSRGPGGFTTNPYDSVGTQLYCVATQFKPLSEKKYQEGVLV
                     GRSKILPKDPWFATIKSCNYLQNVMMKKEAVDRNLDFTIGFDLQNVMTEGSTENIILV
                     NSANELIRPKLRQILKGTTMMRAMHLAEDLLKQKQITRILEQDLHEKDLLSAKEVMMV
                     GTTLDVLPVTFYEEKRIGDGKPGFIAQTLRRYLLEDMKF"
     gene            complement(18591..19640)
                     /locus_tag="VIG33_16830"
     CDS             complement(18591..19640)
                     /locus_tag="VIG33_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY8272561.1"
                     /translation="MDLLSWLTKSNSNAAAEIYVDLGTANTLIAARGKGVILNEPSLI
                     AYTQSSHGKKKVMGVGLEAQEKLAKTAGNIIAQKPIRDGVIADFETTQAMLKHFLSKP
                     GVKSTYSRPRIVVSLPYGVTDVEKRAVVDACKWAGAKQVFLIDEPMAAAIGAGIPVKA
                     AKGNMIIDIGGGTTEVAVIALADIVYCQAVRVGGHRLDEAIVDYFKKYKKFIISENTA
                     EYLKMAIGTAVPKKDIHTATAVGRDAETGLSKSIEVSSEEVGFAMNDSIQEIIDAIHR
                     AIENTPPELVSDIIENGITLAGGGALIRNLNLRIQNEVRLEVRVAENPLTAIAKGGEA
                     VLSDPELLEKIQLEV"
     gene            complement(19642..20316)
                     /locus_tag="VIG33_16835"
     CDS             complement(19642..20316)
                     /locus_tag="VIG33_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272562.1"
                     /translation="MNTLLGRWNLRRYFHWNLHCNLRSALLLSLFFASSSSLAYLSIS
                     ETAELPPANFYQIGFEPQFFTDSGGGTNVTGFFDTLFSDSTSGRLWLGTGVIDFNVGA
                     SVKYVPFPDVDNQPGIGFRAGAFFSRKNSDNSLTLQLAPLFSKKIDSENGLFIPYAAI
                     AMDFINTKEKNFTGTQIMFGTEYKNPEIPKMYFGLETGFNLSDAYSYISGTVTFPFDS
                     SKGLFE"
     gene            20407..21180
                     /locus_tag="VIG33_16840"
     CDS             20407..21180
                     /locus_tag="VIG33_16840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY8272563.1"
                     /translation="MKLPASLPISLVVVTLNEEANIESCLQSVPFASDRVIVDSFSTD
                     RTCEIAQKLGATVIQEAWRGYGSQKAFAVSKAKHDWILSLDADEALSPELQREIVEKF
                     KSLNPEVGYELPRRSFHLGRWIHHGGWYPDAQIRLFHRQHSQWSNDALHEKVQVKSKQ
                     RLQHDLYHWVFDSLSDQVITNDKYSSLGAVNLCKSGKRFSFLKLLFKPWTKFMECYFL
                     KQGFRDGLPGFIIAVGAAYSIFLRHAKLWELEMKKRTRS"
     gene            21177..22133
                     /locus_tag="VIG33_16845"
     CDS             21177..22133
                     /locus_tag="VIG33_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272564.1"
                     /translation="MRILNLMVLVLTLAVAGFSKAAPKYPRIAEVQGKVTWTNKDKQE
                     EKIKVGRLLSEKAALETDVGQIAVQIDAHRRLHLYSHSRLEIPSISWETGETPVILLK
                     WGSFRWQEAAGKTYNVALSSDLFEFLSPQGNFVFSFDPKKAVAEVKVIKGSMEFSALN
                     GEDTALVTAGQKCRFQGVQENDEIVYDVLLKGKKIPRGKLELVQNFSKEEKKLYSEAQ
                     QKKEKVQRLKKEQAEKNSKEPPRSKDEICDQPRGKFNQCAWVCENNAKKEKKTCRLEV
                     PEVHCIRQRCNASGEWAEKVEIPKDKASIFCSAKALVKECDY"
     gene            22314..22390
                     /locus_tag="VIG33_16850"
     tRNA            22314..22390
                     /locus_tag="VIG33_16850"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22348..22350,aa:His,seq:gtg)
     gene            22590..24125
                     /locus_tag="VIG33_16855"
     CDS             22590..24125
                     /locus_tag="VIG33_16855"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEY8272565.1"
                     /translation="MLSIGQFAEMAKVSARTVRYYESIGLLPSSSRGENNYRYYDKKW
                     LERMNRIRDLQSLGFSLEDIKTAISFSNSELRERLKSRLLEIDQEMSSLGDRRERVQN
                     LLFVSTKIEAGEIITETERNLYMESIREEIIEGLKSRYSEVTDSTLAYLKRDGWINNH
                     PQVGAFLEGVKKCVEFAKQKNLTLGTARGSAPASLSLYGLGFSGVDPLKYEMIPERLS
                     TQSPFFHIDVEYERGQEFVDFCRDINRTLTYGEIQAFKMPLIDIVQGVQKSIGKVINY
                     DAIDDNSDVVLNPFRQAEIEKIFQFDFSEDALVMNFERFLPEYLGLQKMTEYLKGQKI
                     YNFRDIINMTALWRPHCKEIAERIELYKKAKNQAFSYRFLSENIENWLKPNFGMIIYH
                     EDLLKIISEHTGWDFARSNSLRRACMGKNHSEVRDQNLDWIEFKRIASKEVVELVAEE
                     SKWAFCLPHAISFAKFTKQTAVLKSLHKEVYFAEIEKFEQKHGITWDDIGIRIKGVSL
                     HQG"
BASE COUNT         6493 a         5170 c         5897 g         6980 t
ORIGIN      
        1 aagagagtgt attttttttg ctgcaaaaac ttgaggacat gaaaaaaata aatcgcagcg
       61 ggaaccaaaa caaaactagg tcgaagcatg aaaagcacaa tcgggaaaaa taaaaaagac
      121 aaaacgaagt agagcttcac aagaaagctt gcagcgccgc ctgcgttctc ccgaagactt
      181 gaccagcgca aaaccgagaa gaaataaaaa gtgaaaaagc taatccctag gctttcggtc
      241 aagatcgtgg gagcgaattg cagcagttca aagttggttg caaaatacag gctgccggtc
      301 caaataaata tttctttagc ctttgctgac ggaaatttct tctgaagcgt ctcctgaagt
      361 gtctctatga ccatcctgca aatcaggtga aaagctcccg cagaaagggc gaagatcagc
      421 atttgaaaga tcgcggtgtt ttccgtggtc tgaaagacgg aaagtaaaaa aggataaagc
      481 ggcgtgcgat gggggtacca gcttaaataa gatccgctgt caggaaagag cttcggaaac
      541 tcccccggca agatgacaag acattgatga acgaagctca aagcacagta gaaccaagga
      601 gtgttcaaga gttttttcgt cattagtgtt taactccttc cccattaaac actttgtaag
      661 ccgtgcgaaa aataattttt aaatccagca agagagaggc gtttttaaag tagtaatgat
      721 cgaattccac tttgatcggg atcggcaatt catcgcggcc attgatctgg gcccaaccgg
      781 tgattcccgg ccgcagaccg ctgactcccc gttctgttct taatttgatg aggtcgtctt
      841 gattaaataa agccggacga gggccgacga agctcatgtc gccgaccaga accgaccaca
      901 actgcggcag ttcatccaaa ctggtcttgc ggatgaagga ccccaccgga gtcaggtgat
      961 cctgcgcatt ggtcagcaga tgggtcgcga cctggggagt gttgattttc atggatcgga
     1021 acttgggcat taggaaaagt ttattgtctt tgcccaccct tttggaccaa tgaagaaccg
     1081 gtcccgggga agtccacttg accactaaag aaataatcaa tagaggcgct aaaaagatcc
     1141 ccaggagtga cagggccaga atcgtatcaa agaatcgctt cgtcatgggg cgagtataaa
     1201 ggcatcgatt tcagatgaga accccaatta gccttaatgc aatgcttaac tcagggaata
     1261 gtttcagctt tacttcctca agcttgccat ttggttgaag caaaggcccg cgtatgtcat
     1321 gatggactga tgtttttacg aatcgccgaa ttgccccgaa aatttaaaat tctgataatg
     1381 cttggatgtg atgtggtgct tttgcctttg gcgctgtggt ccgctgtggc cctacgcttg
     1441 gggactttta cgcctgatgt ttctcagttt tggtgggttt tcttcgtcat tccctttacg
     1501 gcggtgccca tttttattcg cttcggtttt tatcgagcgg tcattcgtta tatggatgat
     1561 aaaatcatct acaccgtcat ctatggtgtt tcgctttccg tcttgatgct gatggcagtg
     1621 gtggtgatgg caaaacttgt ggctctgccg agaagttcca ttgtcattta ttgggtgatt
     1681 gccatcgcct atataggcgc cagtcggttt gtggcccgag gtctgattcg aaccattgaa
     1741 acccgcagag atcgcaaaca aaacgtcgct atttatggcg ccggccaggc gggcttacag
     1801 acggctttgg ccctggtttc gggacgggaa taccgacctg ttgctttttt tgatgacaat
     1861 ccagagctgc aaggaacttc cgtcgctggc ctgcgcgttt atcctccgaa agaggccctt
     1921 aaaatcatgc agcaaaaagg ctgtgagcag cttctgattg cgatgccgtc ttcttcacgc
     1981 acagtgcgta aagaaattat cagccgcttt gaaggcaaag atattgtgct caagattttg
     2041 cccggtctgg gggatttggt gcagggaaaa gtgcgggtgg aagatattcg tgaagtggga
     2101 gtggaggatc ttctcggacg cgatcccgtg cctccctttg aacaacttat ttcttcttgc
     2161 attaagggac gggtggtttt agtgacgggg gcggggggct ccattggctc tgaactttgc
     2221 cgacagattc ttttaaactc gcccctcaaa ttgattctgt ttgaacggag tgaatttgct
     2281 ctttataaaa tcgagcaaga actgacccgc ttgcatccgg aagctcaagt catcagtgtt
     2341 ttaggagatg tgttggatca agaacatctc gagaatttaa tgacggcgca tgcggtgcaa
     2401 accgtttatc atgcggctgc gtacaagcat gtacccttgg ttgaagccaa tgcggattcc
     2461 ggaattttga ataatgtgtt cggcaccaga gcggcggcca aagccgctat caataccaaa
     2521 gtttcaactt ttgttttgat ctccactgat aaggccgttc gtcccaccaa tgtgatgggc
     2581 gccagcaaac gtcttgcgga gctggtttta caaggactgg cccatgagcc gcagcaacac
     2641 acccgcatct gcatggttcg cttcggcaat gtgctgggtt cctcggggtc cgtggttcct
     2701 ttatttaaag aacaaattaa aaacggaggc cccgtcaccg tcactcatcc cgaagtgact
     2761 cgatatttta tgaccattcc ggaagcggcg cagttggtat tgcaagcggg ctccatggga
     2821 aaaggcggcg acgtttttgt cctggatatg ggagagcccg taaaaatcgt cgatttggct
     2881 cgcaaaatgg tggagttgag tgggctggaa gtgcgtgagc cccattctga gaacggcgat
     2941 atcgccatcg aattcgtcgg ccttcgccct ggtgaaaaac tctacgaaga actgttgata
     3001 ggcgacaatg tcacctggac tgagcatccg cgcattatgc gtgctgaaga acatttcttg
     3061 gaggagaatt tcttaaaaga aaaactcacc gaaatacagg cggcttgtaa acagggggac
     3121 tcgataaaga tccggggaat tttaaagagc cttgtgaaag agtacacttt agaaactttt
     3181 aaccgatagg ttgtttatga tagttcttat agtgccctgt tataatgaag aaaagcgtct
     3241 ggatctggag gcctttgaag aaggggctca aggtgaaatt gagatcttat ttgtggatga
     3301 tggttcgaaa gacaatacgg tttcgcgtat agaaaagttt attgccggaa aaccgaattt
     3361 acatctctac tcttgtgcaa aaaatggagg caaagcggcg gcggtccgtg agggcatgct
     3421 gtatgcgatt caacatcccg ctctctctca agcaaattgg atgggttatt gggatgcgga
     3481 tctggcgacg cctctttggg aagtccccaa tatgatcaaa tacgcggagc tttacgatac
     3541 aaaggtggat tctatttggg gaagccgggt gtatcgtctt ggcagtaaga tcgtgcgctc
     3601 tccgcatcgc cattacctgg ggcggggatt tgccacagtg atttcgctta tgctaaaggt
     3661 ggaatcttat gattcgcaat gcggagctaa actgattcgc cgcgaacata tccaaaccgc
     3721 ttttggcgaa agcttcttaa gcaattggat ttttgatgtg gagatcatgt tgcgtttaaa
     3781 agggaagctg ttggtggaat atccgcttcg gcaatgggaa gatattccgg gatcaaaagt
     3841 aaaaatattc cgtgaaatac ttcgcgtttt ccaagacatc ttgaaaattc gtaaaaaata
     3901 tcttggttag tcattctagt taatcaaaca gagacatctg cgaagagctt ttcttcttcg
     3961 gttttctgaa aggtcttttt tgtgactttc tttttttagc ggaagcccgg agattcggat
     4021 tcgaagcttt ctcatagaaa ttatgaatat ccttgatctg ctcgatatct ttgagaattg
     4081 gcatcagatt catttgaaat tctttttgaa ttctttttat caaagcatca taagtgcgtt
     4141 tagtggaaaa tttccaggct ggatgctttt cataagattt caggctggcg cgaagctccg
     4201 ctaattcttc ctcaagccat tttgtgctgg ctttaatttc gccgaaagta agattgttca
     4261 attctttcag gcgaattgtt gccatgattt caagtcttct caattgatga agatctttgt
     4321 ttttataggc ttcttgaact ctatgccaga ctttattgta ccaagctttt aaattctcat
     4381 tgagcttagg gtcctgagaa tctggatgaa taagacgaac caatttgcgg aaaacaaatt
     4441 ttagatctcg ttcctcattg tcagaaagag agaaggaacg acctggggag gtgcggataa
     4501 aatcattatc atcagaatct tcatttgttg aagactcttc gaaaggctgt ccattgaaaa
     4561 gttctcggag ctcttcaata aacttctcca taggtgtccc agtgttcttt ttaaagtctt
     4621 tggatagatg tcgctgagtt tttaatgata aaatatccca aattttaatt aaaagagagg
     4681 ggtcttcaga aagggaagcg atccgcatgg cgttcatggc caagaaatga gcttctggtc
     4741 cagaaaattc tgttcgtaag tgatccggat ccgttgattt caggtgatgg tacagcttgg
     4801 tatctatttt atttaaagcg tctttattta aagcctcaaa atcatcctgg ctctccccct
     4861 catcatcgtc ttcaaaatca aagggtgagc cgaattcttc gtccatagct ttttgcgcaa
     4921 actctgatct ttcctggcgg agactgtcta ttttcgctcg ctgagtttct gtgcccgctt
     4981 gataacgctg ctcttcttct tttaaaaagt ggtaagcccg gtgaggaggg atattctgca
     5041 tttccgcgac ggcaatgata tcattgtgga atttcgctaa tgaaatgtat tcttggcgtc
     5101 ggtcttccat ttcttttttt tcctggcgaa aggtcaaatc atgccagtcg ttgaataaac
     5161 gttgatctat attagaaaaa ttgtcgcgat cacgttcaac cttgtcgatt tttttgatga
     5221 tggattccgc ttctgagaaa agcttcaggc gcagtcccca atcatcgata accagtaagc
     5281 ttctagtcgg attggtcctt aatgcttctt tgagttcgcg gtattttctt cccatgcttc
     5341 caacctctat cagagactga ttgatctagc tactgatttt tgtaaatgac ttttattttc
     5401 gaaatgatga aatcaggatt aaatttatag tttaaacttt tgtgataaaa gccgtgttaa
     5461 actcaatact ttcgtggttc agccaatcta aaatttttat cggcctatgg atcaatccca
     5521 tattgtcgat gacgggagcg tcgagtttgc tgtaaccgag aaggctgcgc aggctgctgt
     5581 attcgtactc ttccactgtg cgacagaggc cggcttcgag gggatttcgg taaacatatt
     5641 tataggcatt cttataatat tccgcactca agatcggatc gcagaataaa ggctcttcca
     5701 gggcttccca ggttttattg cagagagtgg tgagggtttg atgaaattcc tcggctaaaa
     5761 tgtgctcttt ctgggagcgg gtggaaaata aaacatgaaa gtgattaccc attaacacaa
     5821 aagcgtgcgc cgcgaagtct gtcttaagtt gaatctcatt gagcgccaag cagaagattc
     5881 tccaccgttc tggcattgtg gttgtccgcc aagtccgatc ggagagtccg gagttcagat
     5941 gaaaaaattt taaatccttt tgaaaacgac gtggagttat catctctggg taaagagcaa
     6001 attcaacgcc aggacgatga gcttaatctg ggtcgaggtg aagtcgggaa acagtcgaat
     6061 taagaacctg taataagtta ggaaaggggt taggaagaag tgaactctca agctagcgaa
     6121 aaaggcggtt atgaacgaga tgaatgcgat gcaaaaaaac ctgcttatct tgaactccca
     6181 ggcaaaagca ttggggacag tagagctttt tctgagaaat gcaaagagat gcggcattgg
     6241 cggtggcaaa atataaggtt cagaattatc taagaattat ctaaatgata ggatcgatca
     6301 tcacgagaaa actgaagcgg acctcagcaa aatcaaagcg gcctcttagt ttagatattt
     6361 aaagcagcat tttgataaat gacacatcac catatcctgg aaatagccct gaataaaaga
     6421 taaaatagga ttttaatttt tcccaagaga tagatatttg ggcacttatg aaaagttact
     6481 cagacataac gaaaacgatt ggtcaaacct tctggattgt tgttatagtt ccgagccatg
     6541 ccgaaagata tttatcaact tcgttatctg agcaggagct tgcagaggct cagcagttaa
     6601 cagtttccac tgtatcggat cagtaccttg ccaaattcag ttaagaaaac aaaaaataat
     6661 tcgaattttt ttaccgcacc tttctatgtt ccttcggcca gtgacagtct tatcagcgtc
     6721 ggagcattca gtgaaaagac tttgccagag aaggatctca aggtcttgat ttggaatgtt
     6781 tataaaggca aggatccccg ttgggaaaaa gaattccgcg atataatccg gggtcatgat
     6841 ttggtcttgt tgcaagaggc ggtcacagat catcgcatgc ctaaaatatg gcgcgacgat
     6901 tttgctcaat acgcctggaa tatggcggcg agctttcatc tcaatcctca ggaggccacg
     6961 ggagtggtga ttggggcgat ggcggagtct ttcgacttga aatttgtgcg caccaaaagt
     7021 cgcgaacttt ttatcctgac tccgaagatt gcgttgctgt caaaatttaa aatttccgga
     7081 cgaaatgaaa agctgatggt ggtgaatacc cactctctta atttcaccac gacgtctttg
     7141 tttattaaat ccgtggaaga gaaggttcag caaattgaat cgcacaaagg acctttgata
     7201 ttcgccggcg atttcaatac ctggaatacg cggcgctgga ttttattggt gcagattttg
     7261 tcggagttaa agctctatcc cgtggatttt gcaaacgaca cgcgtttctt taagctcgat
     7321 catgtctttg tccgaggcat tgatattcat cagaatcagg ttctgcatgg attcaagggt
     7381 tccgatcata cgcctttgga agtggtcatg agttttgaat gaggagttct ctggggaagc
     7441 gaccctttaa ggcttcatat tattatggtt cagttcgggc tttagggcga aataagtgga
     7501 cgctttgcct tgttgagcca aatcctcgtt gatttgttca acagcctttt cgaagttatt
     7561 catgattaag cgttgttcgc tgggggtgag tttaacttcg ctcgagttcg cggtgatcag
     7621 ctggatagag cctttgttaa gttccgttgt tttcatgtca acgccgaaaa taactccctt
     7681 gagcttcatg ggttccattt tatcagcaag ttttttacga agaatttttt cgatggattc
     7741 tccgtgcaca ctgagttgat ttcgtgccgc tacctcagtg acattgtctg gaataaaact
     7801 gagacgaaac tgagaggaat agcccgcatc tgaaagcctc tgaagaatct ttttcttttc
     7861 gccgtctttc aggatctgct tgggaatggc aatgcaatca tcgccgctgc agacagtttt
     7921 gattcgctca ggtcccagga cctctttgac gacgtcttga aaaacctgct tttgcttggc
     7981 aaattgttct gtgactattt tctcggcttt gcgagtttgg actaaagctt catccaagtc
     8041 tttcgcattg ctaagggcac tggcggtgcc gctgagattc tttgctccca ttcctgccat
     8101 gtccgcggaa aaacctccga agacagcctc atctaaattc gcaacttggc gttcggcaat
     8161 atgaatgcca ggagcgaatt catccacgga ttttgcatag gcgtgcatct tttgaaccgt
     8221 ggaagtggag agtttttcga gtccaaaacg gtttttaaga tcggcttgtg atttctgaat
     8281 atcatcgccg tttttgcgga ttatatcgag agcatcggag gtgagaactt ttttatcgct
     8341 gccttcgacc atggaagttt tacccaattc attttgcaag ctggctcgct gtgactcaac
     8401 agctttcagc tttgtcgtca tagcggactt catctccgcg gatgaatagc tgtgcagagt
     8461 gttttcagcg ctttgacggg aaaagcgtga ggctaaatta gcttgatccg cggttccacc
     8521 gaatccgcca cggaaccaat tctcagggtg agttgaggga tcaataaatt tattttttgt
     8581 aacatattga gtgaactcat cattggtttc attaagaatt ttattcaaac gttcgttcag
     8641 atcggggggg gcaccccctt taaaagcgaa acgactggct ttgaaatccg agtaagcgga
     8701 gatctccaga tctggatatt cccacttcag ttcgtctaac aatttcttgg tttttgtatg
     8761 caggatttct ttgtgatagt tggtaagact ggttacccaa tttttatctt tgaccgtgtc
     8821 attgagggtc tttaactcag agttctccac gtcaaagaaa tggatatttt tgctaacggt
     8881 cgatttctcg gcctggccca tccattccag attttggttt ttatcagttg gggagtagtg
     8941 gagatacttg gcggtgaatt gctctttact aagggctttt gtggcggcaa taccggcatt
     9001 tccgatatct tgagccatgt cggactcttg atccaaggct ttaagaagac ggcgggcctt
     9061 taatagagcc acactttttc cagcaatcac ggtggggtct acaagcgtgg caaagaaata
     9121 acactgaagt tcttctttcg cttcaggggt atagcagttg tagtgatggt acttttccgc
     9181 gctctttgtt tccgccacat gccaaagatc cactaatttc cgcttggttt tttcaggatc
     9241 aaaatgaaca tttcttagat cgggcaagga aggtaaagaa ggcagatggt aatccttcag
     9301 tggtgtggat ttcagctttt gccaggcggc ttcgattgga gcattgaggg cctttagcgt
     9361 gtccataaca gaatttatat tgcccttcca acaactcaga gcttcgccga catcagacat
     9421 gatctgctga tggcagaagt cctggggctt acaactcatc aataaatcct tgatctccgg
     9481 atgttcgccc gcaatctttt cgcagttatg agctttggcg ttgatttcac aagcctcgag
     9541 agacctcttt aggcctggcg gaattttttc gcactttgca ttgaagtaat ttctgcaatc
     9601 aatctccaag accaaatttg atagccgatc aacggcttga acctgcaatc cgatagctaa
     9661 acaaaatatt atagtaatat cgcgaacaca ccgcatattt atataacggt atttttcgcg
     9721 cctctatgaa gagtatttac gaaaagaaag atcgttgaga ggtctgcctt aaaatgagaa
     9781 atcgaggttc tatagaggtg ataataagga tgaaaaagct ttgatttttg ttaaggaaat
     9841 tttatcagag gtctgtgatg aacacgcgtc aataacggaa tagctccgct gaggatggcg
     9901 ccgaggagcc aaacaaatcc tgcctcaaag tggagatcgt ggctcatcat tgaaaaaatg
     9961 ccgagggaga gcagagacag ggcgccaagg ctcagagttg gattttttct tagaacttcc
    10021 cactcaaatt caccgagaaa tattttggca atccaaaaag aagctcctaa atagaaggca
    10081 ccgcaaaaag caaaacaaac tacgctgcca gccttcataa agtagtgcat aagtccatgc
    10141 ccttcaaaga agaggcgtaa accaaattga gggcaggcca tcaacgtcaa cagagagctg
    10201 gcaagatgaa taagccccat tttcaatgca acccgggaag gggcagggaa gatttccttt
    10261 ttgatatttt ctaaaacaga aggattcatc tggcgttggc cagattctaa aaaagattca
    10321 aaatctttca aggtttcctc ctgttatttt gttgcgtgct tctttctcaa gaatttcaaa
    10381 ctccggctga caatttgccg aatattgctt tcactctcat tgagcttctg tgcgatttcc
    10441 tcatactcca gttcatttaa atagcgatcc tggaggatct gtttctcctt ttcatccaag
    10501 cctgatatgt aatattgcac ttcttgatta tcttcgaagc cattatattc tctattagga
    10561 atgtggccga ggttttgtga caccgattcg ttgaattcag cgaaattttt aagttcgcgt
    10621 aatttatagt cttttaactc cgacttggtg atgacataga tccaggccaa ggccgcatag
    10681 tggggatcgt attgaatccg ttttcgataa atttgcataa agacaatttg cacaaggtct
    10741 tgggcctgct cggtgttgaa tttgcgtttg agggcatagt tgtaaacttt gttggagtgg
    10801 cgcttaaaga gataatccag ggctttatta ttaccctgag caagactgag caatagttct
    10861 tgatcacttt tgtttatcat ttgagttact ataaaggaaa atatgcctaa aacacaacaa
    10921 gagcatgaaa ttgcaaacag tcgcctgctc aaagcgattg agatgattga aaaagagacg
    10981 gagcataagg gtctgacact gggagagctt attgaactcc tcggggaaat gggtcatgcg
    11041 gttttaattt tattattgtg tctgtgcttt cttcaaccca ttcctctgcc gggaatttct
    11101 actccgattg gcatcgttat tatgatttcg gcgaccttgc agtttctgaa tttaccgcct
    11161 tggattccgc ggcaatttcg tcaacatatg atttcgcaaa aagtacttca taagactgca
    11221 gtggtggcgc gaaaaatctg gtcgaaaata gagaggtttc tcaatcctag gcttttattt
    11281 ttaacaaaga ctccgacttt tcgttttata aatctagtgt tgattattat aagtgctttt
    11341 ttattggccc ttcctctgcc tattcccttc accaacacgg ttccttccat tgtgatcctg
    11401 tctattgtct ttgctcaact ggaagaggat ggcttttcca ttttgttcgc ttatttcatg
    11461 acctttgtaa tgtcgatttt tttctactct ttaggcgctg gtatttggac attcgcgcaa
    11521 agaccttggg ctagtctcct gtaaagccca aagatgacga gatgaatatt gttgttaatt
    11581 gggtggcgaa ttagttatcg tgctagtaat ggtactagta atggtactaa gccgctccag
    11641 ggctttgtct gaaccaagga ttttcctcca atgaaatttg tgatatttgg aaaaagtgtg
    11701 agcagtgttg agcaaagctt aaagctgtcg aacctcgatg ctttcttgta tagcttgatg
    11761 gtgggagcag gagaatctta tttgccggcc tatgccctct ctattgggat gggtgaagtc
    11821 tttgcgggaa ttttagcttc tttgcccttg gtcagcggcg cggtcttgca actctttacg
    11881 ccgcgtttat tgcataaagt tcattcgcac aaatactggg tggtgctgtc gacgttgttg
    11941 caggccatgg ccttcttgcc gctgatttat ttttcattaa cccgggcacc gaatttttgg
    12001 gttttatttt ttattttcac tctttattgg ggggcgggat tttctgcggg ctccgcttgg
    12061 actttctgga tggggcatct tgtcccagag aaagagagcg ccaagtattt ttctttccgt
    12121 gcacgaattc agcagatagg gattgttctg ggaatcgtag ggggtggcgt ggctcttcat
    12181 aacaaggtgg agatcggtcc cttttcgtcg gtgttctctt tattgtttat ttttgctttt
    12241 gtctgtcgcc tgacttcaac actcgttctt tctaagaaaa tgtatcgctc tgaatgggtg
    12301 ctcaacgatc aggtccaccg cttgcgagac tcctggagct tgttttgggc aggccagcat
    12361 aagaagaagt tttttgccta tttgattcct tttcaagtgg cgatttacat gtcttcgccc
    12421 tttgtaactc cttatatgtt ggcacagttg aagatgaact atggtcaata tatgggggcc
    12481 atagcggcgc tcatgattgg aaaaattatt tcactctcct tcatgcaaaa agcgaaatcg
    12541 aatttcgacg ggtttaaagt gatgatgttg ggcttggtct tgatctctcc attgcccgcg
    12601 ctgtgggcat tgaatgaagc attttatttt gtcgtgggct tacagatctt gagcggaatg
    12661 ggatgggctt gctttgaagt gggactcgct ctggtatttt ttcaagatct tcgccaagag
    12721 gaaaaagttc cagtgctgac aatttataat ttattgaact ccgtcgctat cattgttgga
    12781 acttttttag gagcaaggtt cctacatatg atgggtgagg taaaaaacag ctattggata
    12841 ttatttgttg taggcgcctc tttgcgagcg gttttctgcg ttccagtagt tcttcaatct
    12901 cgccgctgga aaaagatttc cgaccagcaa gaagctgcag cagccccaga agaactctta
    12961 atttccaaag tgtactaaaa actcacgatg aatattttta tcaagatagg gatcagcggc
    13021 agcaggggcc agatctgcct cgctatatcc agaaaataat ttaaccaccg tgacctccaa
    13081 cttttcctca tccactgtaa actgcactcg ccaagtttta taagccaaga cgaagtgatc
    13141 ttgaacagcc gaaactcttt ttttatctct attgatcgga tcatattcaa gctgattcaa
    13201 aataaagtct tgcaactgcg aaagccccct ctcccgcaaa aacccaacct gcaaacgcgc
    13261 tgcgatacta gaaagtacgg cgtaccttct aggattttcg agccagccgg ttttggagga
    13321 ggggtgggag tctgcgtagg ggagataagg tttgatgtcg aggatggggg tgccgtccag
    13381 aagatcggcg ccctcgacga ggaggcgaag gtcggagact tttagaagct tgacgcaact
    13441 caggccgatg ggattgggac ggtacggagc gcgggttgca aagaggccca ctttggtggt
    13501 gctgcctcgg ggaggaagaa ctttgggttt ccaatggata ttgtgatgga aaatgtaaag
    13561 gagccacacg cgttctatgc cctcaagatt ttccagggcc tgttcaaact gctggcccga
    13621 gcgcagcgtg atttcaccct gagttgttac gcgctcaagc ggttgcctcg ccgcctcgta
    13681 gggctccttg gcgtcggaat ggtaaatccc aatgggagta aagcttactg aggaattcat
    13741 agagaactcc atccattgta ctgactcgta tactgacgcg aaaattcgta ggcggcaatg
    13801 gccgcagcca ctcccacatt gagggaattc ttctgaccgc gcaaggggat tttgcgaacc
    13861 tctgtacaaa gctgaaggat ttctggatca attccaaagc gctcattgcc aagcacgaag
    13921 gcggttggca tgtcgggaaa aacattttcc aagggaagcg catgggaggc ggtttccaaa
    13981 gccacaatat tataattatt gttccggcga agtcgtaaca aaagatccgc gatcttgggg
    14041 atttcctgcc attccaccaa agtatctgtg cccatggccg ttttcgccaa ctggtcttga
    14101 gtgggaagtg gagtatagcc gcaaagatag atcttgctcg cgccaaaaca ttcggcggtt
    14161 ctaaaaatag aacccacgtt aaaggcggaa cgcaagttgt gcagaatgaa aaccaaggga
    14221 attttttctt gagctttgtg ggcgccatcc tgagagaaga ctaaaaactc gtcatctcgt
    14281 aatttttttt gaaggagtct ttccactgga acaatccatg aaacataatg tttcatagtc
    14341 atctctaaat gaaggtgagg cgcaatttca gaaatcttgc gaaattcaag atcagggtga
    14401 gtgatgaagg cctcaaggtc tttttgcagt ttgcttaaag tctcaagact aaaaaagccc
    14461 tgcgcggccg cccgttgttc gagctcgcgc atttgtctgt gaatattctg cagggctttt
    14521 aaatctttgg gatgcaaatc aaaatcccat tcgcttattt aacacgctgg ccagtcttgg
    14581 ctccggaatc gggagaaagc acgtagagat cgcttcctcc ttcacctgca gctaagacca
    14641 tgccttcgga cataccgaac ttcatcttgc gtggttttaa gttcacacac accagaacct
    14701 gacgacccaa gagtttatct ggagagtaag cggccttgat gcccgcaatg atctggcgga
    14761 gctcgcctcc accaatatcc accttcaagc gcagcagttt gtcggcctct ttgatttcct
    14821 cagcttcgac cacggtgcca atgcgaaggt cgatcttcat aaagtcatca aattcaattt
    14881 ccgcagattt gatttcactg cgatttttaa tcttgatgcc acttgcagag gctgtggcag
    14941 actgcttctc gctggcgatt ttttcgttca gcgctttggc ttcttcgacc atcgctttga
    15001 ccttgtccgc gtcaatacgg ctggctagat gttcgtactc ttgaattccc gtgcccaaaa
    15061 gaattttctg agcatcgacc caagaatagg gcttctcgcc aaaaagtttt tcaaccctcg
    15121 ctgtgtaatg cggcagaatt ggcttgaggt aaatactcaa tatgcggaag atgtttaaag
    15181 ttgtcgtcag aacttcttta gtgctctgag ggttgctctc cagagttttc catggagctt
    15241 tttcatcaaa gtactggttg gctttatccg ccagagcgcg gatttcattg gtggccttca
    15301 caaaatcacg gccttcatag tgctttgcaa tctcttcgtt tttagcttga gcatagcgga
    15361 tgagttcttc accttgtttg tctggagcgg tcatgactcc atccattttc ttttttaaca
    15421 tctgggcccc gcgagaagcc agattggtga tttttcccac caaatcagaa ttgacgcgag
    15481 tgacgaagtc ttccagattt aaatcaatat cctcggcggt gccattgagt ttggttgcat
    15541 agtagtaccg caaaaattca gggttcaagt gattcaaata agtgcgagcc gcaataaaag
    15601 ttcctttgga tttagacatt ttatcgccat tgaccatcaa gtgaccgtga acaaaaacct
    15661 gatctggaga tttaaactcc gccgccttca agaaagcggg ccagaagata gtgtggaagc
    15721 gagcgatgtc tttgccgata aagtgataga tctcacgggt gggatcttgc caaatatccg
    15781 caagcgtttg gctttgcttt ttagcccatt gctcggtgga ggacatatag cccattggag
    15841 cgtccaccca cacatagaag aattttttat tattcgtccc tggaatcgca aagccgaagt
    15901 aaggctcatc ccgggaaatg tcgagatcgt gcagatcctc gttaaaccac tccaacattt
    15961 ttttgccaat atcactggcg caatgttggg ggatccactc ttcgagatat tttttatagt
    16021 cattcaactt aaagaaaata ctttcgctgt ctttcagcac cggcgtattt ccgcaaatag
    16081 aacaatgcac atcttttaga tcggcgggag cataggtggc gccacagaca tcgcaagaat
    16141 ctccgtactg ttctttagag ccacagcttg ggcaagtgcc tttgacaaag cgatccggca
    16201 aaaacatttt gtcgtgttcg caatagagtt gcttgatcgg acgagagaca atgtgatggc
    16261 cgtcctggaa cttcttatag aactgttcaa tcaagacgcg gttttcaacc gagtttgtgg
    16321 agccatagtg tgcgaactca atctgaaaat ctttaaagtc ggtggtatgc tcttcgtagc
    16381 tttttgcgat caattgctcg ggagtgatgc cttgctcacg ggccttcacc atgatcggag
    16441 ttccatgggt gtcatccgcg cagataaaaa tgcactgatg tccgcgcata ttttgaaaac
    16501 gattccagta atcggcttga agatactcaa cgaggtgacc gatatggata tagccattgg
    16561 cataggggag cgcacaggtc attagaatct tacgtggcaa gggtcgtggg gatatgctca
    16621 aagtgttcaa aaaacctccc agatgaattt ctatgttcta cttgaactca acgactccag
    16681 agcccagatg gtacaaggcc gtttggattt ttacctcacc agtattaagg gcgtcacgca
    16741 cgatttgaga tcttccgatc aaatctttag ccactccttc ggcattggcc cagccttcga
    16801 tcagggtccc ttcagagagg ggttttcctt caaaactttt gagatgtgga cgaagattcg
    16861 ccatcaatct gttcattgag gggctgcccg catcctcgcc gccgagcgtt gccaacgcgg
    16921 ttttgacggc ttcacaggat tcgtgtccta aaaccacgat gagattgctg ccgaagtgtt
    16981 ctaaaccata ttcaatgcta gccaccgccg ccgtatccaa ggattcgcct gcagtgcgga
    17041 tcacaaaaat ttctcccagc ttttgatcaa aaacaatttc tggtggaacc tgagaatcac
    17101 tgcaagaaat aatgattgca tgaggatttg ggctcgacgc cacccgggcg cgatcttttg
    17161 cggtgtctcc atcattgcgg agagagtgtt tcacaaaacg aatatttcca ttctttaacc
    17221 agccgagagc tttctcggaa gccacaggac cccggggaac tgagtgcttt ttgctcggca
    17281 ctgttttcgg ctttgccgcg gaaaccaccg cgggcgtcgt aatctcggag ttttttttag
    17341 gcaccttaag ctcttccagt tccttatccg cttgtttttt cagaggctca gtgcctgcac
    17401 ttaagtgagg ggggactgga ctttgagctg atggagcgtg agccgatgaa gtttctgcgg
    17461 ctggcttgct actgcaattc acaagactga gcccggcgag aatcatcact agaattttca
    17521 taataaattt cctctctgaa aataatgcgt ttaatataga ggctccatag taaaccgatg
    17581 aggtttaaaa aaggagcttt tgtgagtgtt cctctgctat cgtccgatag gattttgaaa
    17641 aaactagcgc tttactctta cgagcaaaaa aagacttatc tggcgatgta ctcttcctgg
    17701 tatggcggta ttactaaaga cccggctttg atgatggtgc ccgtggatga ccacctcgtt
    17761 catcgcggag acggggtctt tgaagccatg agatttctcc atggaaaagt ttatttaatg
    17821 aatgatcatt tagatcgtct ggagtcttcg gccaaacagc tgggcatgga gtggcctttt
    17881 ggtcgtgaaa aaatggttca gatcattgaa gaagtggtga aggtcgctgg agcggatgag
    17941 ctgatgctgc gcctttttct gtctcgaggc cccgggggat ttacgacgaa tccttatgat
    18001 tctgtcggaa cccagcttta ctgtgtggct acacagttca agccgctctc tgagaaaaaa
    18061 tatcaagaag gagtattggt agggcgaagt aaaattctgc cgaaggatcc gtggtttgcc
    18121 accataaaat cctgtaacta tttacagaat gtgatgatga agaaagaggc tgtggatcga
    18181 aaccttgatt ttaccatcgg ctttgatctt cagaatgtca tgaccgaagg aagcactgag
    18241 aatatcatcc tcgtcaattc cgccaatgag ctgattcgtc caaagctcag acagattcta
    18301 aaaggcacca ccatgatgag agcgatgcat ttggcggaag atctcctgaa gcaaaaacaa
    18361 atcacccgaa tcctcgagca agatctccat gaaaaagatc tgctctctgc caaagaagtg
    18421 atgatggtgg gaaccactct cgatgtgctt ccggtgactt tctatgagga gaaaagaata
    18481 ggagacggca agcctggttt catcgcacaa acactcagac gttatcttct ggaagatatg
    18541 aaattctaaa caagtttaaa actttactga ggattttcta gcattttact ttaaacttcg
    18601 agttgaattt tctcgaggag ttctgggtca ctcaagacgg cttctccgcc tttagcgata
    18661 gcggttaagg ggttttcggc cactcgcact tccagtcgaa cttcattttg aatgcgcaaa
    18721 ttaagattgc ggatcaaagc tccgcctccg gccaaagtga tgccattttc gataatatca
    18781 gaaacgagtt ccggcggagt gttttcaatg gcgcgatgaa tggcatcaat gatttcttgg
    18841 atgctatcat tcattgcgaa gcccacttct tcagaagaga cttcgatgct ttttgagagc
    18901 ccggtctccg cgtcacggcc cacggctgtg gcggtatgaa tgtctttttt gggaaccgcc
    18961 gtaccaattg ccatttttaa atattcagcg gtgttttcgg aaataataaa tttcttatat
    19021 tttttaaaat aatccacgat ggcctcatcc agtcggtggc ctccgacgcg gaccgcttgg
    19081 cagtagacga tatccgccag ggcaatcacg gcgacctccg tggttccgcc gccgatatcg
    19141 atgatcatgt ttcctttagc agccttcaca ggaatgccag cgccaatggc cgcagccatg
    19201 ggttcatcga tgagaaagac ctgtttggct ccagcccatt tgcaggcatc cacgacggcg
    19261 cgtttttcga catcagtgac cccgtaagga agggaaacga caatgcgtgg acgagagtaa
    19321 gtggatttaa ctccgggctt actgagaaaa tgtttgagca tggcctgggt ggtttcgaaa
    19381 tccgcaatca cgccatcgcg aatgggtttt tgcgcaataa tattcccggc ggttttggcg
    19441 agcttctctt gggcctctag cccgaccccc atgactttct ttttgccatg gcttgattgg
    19501 gtgtaagcaa tcagtgaagg ctcgttgaga atgacacctt tacctcgagc agcaatgagc
    19561 gtgttagccg ttccaagatc gacgtagatt tcagcggcag cattagaatt ggatttggtc
    19621 aaccaagaaa gaagatccat attattcaaa aagcccttta ctggagtcaa atgggaaagt
    19681 tacagttcct gaaatataag aataagcatc gctcagatta aagcctgttt ccaagccaaa
    19741 atacattttg ggaatctcgg gatttttgta ttccgtgccg aacatgattt gtgtaccggt
    19801 aaagtttttc tctttggtat tgatgaagtc catagcgatc gcggcataag gaataaaaag
    19861 accattttcg ctgtctattt ttttgctgaa gaggggcgcc agttgtaaag tcagggagtt
    19921 gtcgctgttc ttacgggaaa agaaggctcc cgcgcggaag ccaattccgg gttgattgtc
    19981 gacatccgga aaaggcacgt atttcacact tgcaccgacg ttaaaatcaa tcactccagt
    20041 gcccagccat agacggcctg aagtggaatc actaaacaag gtgtcaaaga agcccgtgac
    20101 attggtgccg ccaccggagt cggtgaaaaa ttgcggttca aagccgattt gataaaagtt
    20161 tgcaggcgga agctccgcgg tctcactgat ggaaaggtaa gccaaagatg aggatgaagc
    20221 gaagaacaag ctcaaaagga gggcagagcg gagattacag tggagattcc agtgaaagta
    20281 ccgccgaagg ttccaacgac ccaacaaagt attcatgaca acatcctttc tggttaaagt
    20341 gagatgttag cgagtcgact tatgtttgac aagacaatga aggcttggga cttttaatta
    20401 atgtctatga aattaccagc tagtctcccc atcagtcttg tcgtcgtgac tcttaatgaa
    20461 gaagccaaca ttgaaagttg cctgcaatcg gtgccctttg cttccgacag agtcatcgtg
    20521 gatagctttt caacggatcg cacctgtgaa attgctcaaa agttgggcgc cactgtgatc
    20581 caagaagctt ggcggggcta tgggtcccag aaagcttttg cggtttccaa agcgaagcac
    20641 gattggattt tatctctcga tgcggatgag gctctgagtc ccgagttgca gagggagatt
    20701 gtggagaaat ttaaaagctt gaatcctgag gtaggttatg aattgcctcg ccgcagtttc
    20761 catctcgggc gttggattca tcatgggggc tggtacccgg atgcccagat aaggcttttt
    20821 catcggcagc attctcagtg gtccaacgat gccttacatg aaaaagttca agtaaaatct
    20881 aagcagcgtt tgcagcacga tctttatcac tgggtttttg acagtttgag tgatcaagtg
    20941 attacaaatg acaaatactc aagcttggga gccgtgaact tgtgcaagtc cgggaagcgc
    21001 ttctctttcc tcaagctcct ctttaaacca tggaccaaat ttatggaatg ctattttttg
    21061 aagcaggggt tcagagatgg tcttccaggt tttatcattg cagtgggtgc ggcttactcg
    21121 atctttctac gtcatgcgaa gttatgggaa cttgaaatga agaaaaggac tcgctcatga
    21181 gaattttaaa tttgatggtc ctagtgttga ccttagctgt ggcgggcttt tcgaaagcgg
    21241 ctccgaaata ccctcggatc gcagaggttc aaggaaaagt tacttggacc aacaaagata
    21301 aacaagaaga gaagattaaa gtgggtcgac tcttgtctga aaaagcggcg ttagagacag
    21361 acgtagggca aattgcggtg cagatcgatg cccatcgacg tttgcatctt tattctcata
    21421 gtcgtttaga gattccttcg atcagttggg aaaccggcga aaccccggtg atccttctta
    21481 aatggggatc atttcgttgg caggaagcag ccggtaagac ctataatgtg gctttgagtt
    21541 cggacctttt tgagttctta agtccacaag gcaattttgt ttttagcttt gatccaaaaa
    21601 aggctgtcgc tgaagtgaag gtgattaaag gctccatgga gttttcggcc ttaaatggtg
    21661 aggacaccgc tttggtgact gcagggcaga aatgtcgttt tcagggagtt caagaaaatg
    21721 acgaaatcgt ttacgatgtt cttctcaagg gtaaaaaaat cccccgcggc aaacttgaat
    21781 tggtgcagaa cttttcaaag gaggaaaaga aactttactc tgaggcgcag cagaaaaaag
    21841 aaaaagtgca gcgtttaaaa aaagagcagg ccgagaaaaa ctccaaagag cccccaagaa
    21901 gcaaagacga aatctgcgac caaccccgtg gcaaattcaa tcaatgcgcc tgggtctgcg
    21961 aaaacaacgc caaaaaagaa aagaagacgt gtcgtcttga agttccagaa gtgcattgta
    22021 ttcgtcagcg atgcaatgcg agcggggagt gggcggaaaa agtagaaatc cccaaagaca
    22081 aagcctccat tttttgcagt gcaaaggcct tagtgaaaga atgtgattac taagagatcg
    22141 cctatggcat tgagttttct caggtctcgg caagggaatg tctaaataca aggcagaaat
    22201 gcaccctaaa ttcagtagtt tagggttttc ctctcgccca aataaagtgc agtgaggcct
    22261 tataatttat ttgcactttc ctcagcaagg ttttataaag gttcctcttt gtggtgactg
    22321 tagctcagtt ggttagagca tcggattgtg attccgaggg tcgcgggttc aagtcccgtc
    22381 agccacccca attttttctc agagtcctag tctaaaaatc aaaaaaacaa taagttctaa
    22441 atagttgaaa acacacaaat gaagatcgag cctcagcgtc gatatttcgt gaaataaaaa
    22501 tgccaactca taatgtcatt tgatttttgg cctattgacc ttgtcgttac gtcaaacctt
    22561 atggtgaatt aatgggaagg aattcaatta tgctctcaat tggacaattt gcagaaatgg
    22621 ctaaggtctc ggcgcgcact gtgcgctatt atgagtctat tggtcttttg ccgtcgtcct
    22681 ctcgtgggga aaacaactac agatactatg ataaaaagtg gcttgaacgg atgaaccgaa
    22741 ttcgggattt gcagagtctg ggattcagtc ttgaagatat caagactgct atcagttttt
    22801 caaattctga gttaagggag cgcctcaagt cgcgactttt agaaatagat caggagatgt
    22861 ccagcctcgg ggatcgtcga gagcgtgtgc agaatctcct tttcgtatca actaaaattg
    22921 aagccggtga aattatcacc gaaacggaaa ggaatctgta tatggaaagt atcagggaag
    22981 aaatcattga agggctcaaa agtcgctata gcgaagttac agactcaact ctggcgtact
    23041 tgaaacgaga cggctggatc aacaatcatc cgcaagttgg cgcgtttctc gaaggcgtaa
    23101 aaaaatgtgt tgaatttgca aaacaaaaga atctaactct tggcacagct cgcggtagcg
    23161 caccggcaag tctctctctt tacgggcttg gtttctctgg tgttgaccct ctgaaatacg
    23221 aaatgatccc agaaagactt tcgactcaaa gtccattttt tcacattgat gttgaatatg
    23281 aacgaggcca agagtttgtt gatttttgtc gtgacatcaa ccgaactctt acttacggcg
    23341 aaattcaagc attcaaaatg cccctgatcg atatcgttca aggagtacaa aagtccatcg
    23401 gcaaggtcat taattacgat gcaatagatg ataattctga cgttgtactg aatccgtttc
    23461 gccaggcaga gatagaaaaa atatttcaat ttgatttttc agaggatgct ctggtaatga
    23521 attttgagag gtttttgccc gagtatttgg gacttcagaa aatgaccgaa tatttaaagg
    23581 gtcaaaaaat ctataatttc agagacataa tcaatatgac agctctctgg agacctcact
    23641 gcaaagaaat tgccgaacgc attgagcttt acaaaaaggc caagaatcag gcgttttcct
    23701 acaggtttct ctctgaaaac attgagaact ggttgaagcc gaattttggc atgatcatct
    23761 atcacgaaga tcttctgaag attatttctg aacacacggg ctgggatttt gcgcgaagca
    23821 actctctacg cagggcctgt atgggaaaaa accattcgga agtcagagat cagaatttag
    23881 attggattga attcaagcga attgcatcaa aagaggttgt tgagttggtc gccgaggaaa
    23941 gcaaatgggc tttttgtctt cctcatgcga tttcgtttgc caaattcacc aagcaaacgg
    24001 cagttctaaa gtctttacat aaagaagttt attttgcaga aatagaaaag ttcgagcaga
    24061 agcatggaat cacttgggat gacattggca tccggatcaa gggtgtttct cttcatcagg
    24121 gatagatgat cccgagccat catggggtgg acgtttttat ctaccccaga ctcttataaa
    24181 tcaccttagt tagtgatgag aaaaatcctt tgtaaccatc tgattgaatc tgtaaaacac
    24241 tgataagaac ctagagtact tagcttcgcc tttgaaaggt aacattcctt tctgtttcct
    24301 taatgagttc atagggcgcc catgtcatgt cttcaactaa gttgcttcgg tttgtaatca
    24361 gatgccgaac ataatcgaat tttgaaaact gccccacgaa gtggaggcgc cggttttgaa
    24421 aggggccaat tccaccggtt taccggtccc atggggacca tagtcaccgg tttgaaatgg
    24481 gaccagcgcc taaaatgcca agcaagatcc tcggccttga tggtgaatca aaccgatgct
//
LOCUS       DATBTE010000443         2076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1427392, whole genome shotgun sequence.
ACCESSION   DATBTE010000443 DATBTE010000000
VERSION     DATBTE010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427392"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VIG33_16860"
     CDS             complement(<1..785)
                     /locus_tag="VIG33_16860"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HEY8272566.1"
                     /translation="MSKITKSSTAEYFAKNLQQVGFSSPLKAVLTTLKEAVDNSLDAC
                     ESARILPDLLIEVAKVGSGSTKNTDLIRIVVEDNGPGIDGDDLAKVYGEYLASSKFGR
                     GQCSRGQQGIGISAATTWAQMTNARGVQVISKTKNMRKAISAQVDVDIKSNTGILKNK
                     ESLEWDRPHGTRVEFVLDGRIQLNGDGGLVTYIEGTVLVNPHMTITYKLAENDFVSIA
                     RVSNESPELPEATLPHPHTFKLGEFITHATLFGKTSLSKFLKTG"
     gene            complement(931..1254)
                     /locus_tag="VIG33_16865"
     CDS             complement(931..1254)
                     /locus_tag="VIG33_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY8272567.1"
                     /translation="MNMFFQVASPEHQKREKVKARELRQSQWWRQQIGQGICYHCKQT
                     FKPQELTMDHLTPIARGGKSNKKNCVPSCKDCNSKKGYKMSVELTMENMAKSRSEDSS
                     EDEVE"
     gene            1285..1785
                     /gene="msrA"
                     /locus_tag="VIG33_16870"
     CDS             1285..1785
                     /gene="msrA"
                     /locus_tag="VIG33_16870"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY8272568.1"
                     /translation="MAQIEVATLAGGCFWGMEDLLRKLPGVLESEVGYTGGTVPNATY
                     ELVKTGKTGHAEAIQLRFDPQILSYENLLLFFFKIHDPTTEGQQGNDIGSQYRSAIFY
                     HSENQREIAQEILDRVNKSKAWKFPLVTEIKPLEKFYRAEDYHQDYLEKHPAGYTCHF
                     LRNLNF"
     gene            1869..>2076
                     /locus_tag="VIG33_16875"
     CDS             1869..>2076
                     /locus_tag="VIG33_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272569.1"
                     /translation="MSVLLAFIYPEEKIADDVLETVEKLAREGHIDLEDACAIVKDDK
                     GKIHLHQETNLSFFGAISGLVLGAF"
BASE COUNT          575 a          466 c          431 g          604 t
ORIGIN      
        1 cctgttttaa ggaacttcga taaggatgtt ttcccaaata aagtggcgtg agtgataaat
       61 tcgcccaatt taaaggtgtg aggatgaggc aacgtagcct caggcaactc aggagattca
      121 ttgctcacac gagctataga aacgaaatca ttttccgcca atttataagt tatggtcata
      181 tgaggattca ccaaaacagt gccctcgata taagttacga gaccgccatc gccgttgagc
      241 tgaatacgac catctaaaac aaattcaaca cgagttccat gcggacgatc ccattccaaa
      301 gactctttat ttttaagaat ccccgtattg gatttgatat ccacatccac ttgcgcagaa
      361 atcgcttttc tcatgttttt ggttttagaa atcacttgga cgccacgggc attggtcatt
      421 tgcgcccagg ttgtagccgc ggaaatacca atcccttgtt gtccacggga acactgtccc
      481 cgaccaaatt tcgaagacgc taaatattct ccgtaaactt ttgccagatc atcgccatca
      541 atgcccggac cattatcttc gaccacgatt ctaatgagat ccgtattttt tgtagagcca
      601 cttccaactt ttgcgacctc aataagaaga tccggtaaaa ttctcgctga ttcacaggca
      661 tccaaggagt tatccacggc ctctttcaaa gtcgtcaaaa cggcctttaa aggagaagag
      721 aagcccacct gctgcaggtt tttcgcaaaa tattccgctg tgctgctttt tgtaatttta
      781 ctcacggatg atttattcct tttaatttca ataggttata tccaagatca actgaagacc
      841 taaagaatgt atatttctag gttaaaccta ttgcagagtg ttaaagcaag acttttgaag
      901 ttcaggctta tttttgtaat gcaatgtgtc ctattcaacc tcatcttcag agctgtcttc
      961 agacctactt tttgccatat tttccatggt gagttcgacc gacattttgt agcctttttt
     1021 agaattacag tctttgcaag atgggacaca gtttttctta ttggattttc cgccccgagc
     1081 aatgggtgtc aaatgatcca tcgtcagctc ttgaggttta aaagtttgtt tacagtggta
     1141 acagattcct tgtcctattt gttgtcgcca ccactggctt tgtctaagct cgcgggcttt
     1201 tactttttcg cgtttttgat gttcggggct tgctacttga aaaaacatgt tcatagattg
     1261 aactctatac cacaaggagg acctatggcc caaatagaag ttgcaaccct cgcaggaggc
     1321 tgcttttggg gaatggaaga cttactgaga aagctccccg gagttttgga gagcgaggtc
     1381 ggctatacgg gaggcacagt tccgaatgcc acctacgaat tggttaaaac cggaaaaacc
     1441 ggccatgccg aagcgatcca gcttcgattc gatccacaaa ttcttagcta tgagaacttg
     1501 ctgctgtttt ttttcaagat tcatgaccct actacagagg gacagcaagg caatgatata
     1561 ggcagccagt atcgttcagc cattttttac cactccgaga accagcgcga aatcgcgcaa
     1621 gagatcttgg accgcgtaaa caaatccaaa gcctggaagt ttcctctcgt caccgaaata
     1681 aagcccctgg aaaaatttta tcgagccgaa gattaccatc aggattattt agaaaaacat
     1741 ccggctggtt atacctgtca ttttttgagg aacctgaact tttaatttcc tagaaggatc
     1801 ataccactgt ccgatagcag cgatattctt tggcttcaat aaagcaataa taagaggagg
     1861 aggttgccat gtctgtccta ctcgccttta tttatcccga agaaaaaatc gctgatgatg
     1921 ttctggaaac cgttgagaag ctcgcacgtg aagggcatat cgacttagaa gacgcctgtg
     1981 ccatcgtcaa agatgacaaa ggaaagattc atttgcatca ggaaaccaac ttgtcttttt
     2041 tcggagctat tagcggtcta gtcttaggag ccttta
//
LOCUS       DATBTE010000444        19683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_744207, whole genome shotgun sequence.
ACCESSION   DATBTE010000444 DATBTE010000000
VERSION     DATBTE010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 19683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19683
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744207"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..2130
                     /locus_tag="VIG33_16880"
     CDS             292..2130
                     /locus_tag="VIG33_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272570.1"
                     /translation="MTRFYAGSIKHFGYSFLNAILTTSLILGPCVGQGAGTPVPDNTQ
                     NQTNTSVEAIIPRIEGVDLDTVEIAPDGVAVSLPAPLEKIAQEQANAANAAPAWVKEL
                     RSRSVGEYFPPNLDDKSEAWRQGKQRLLTDAQNLHKYFEEQDKSGVYKEFIDKKLSPF
                     VNTLDAKKIAASIRDQRITRPIKEWTVETLVVTEQIAYLGRYPNFVKRTMSLFSGRHL
                     RGASAVAGTLVLSAVGLFTAYHFGYHFDAATLSAGAHAVGTGIKSVTSAVDMKALSMG
                     AFSTGIAVGAFLGRALGGAFQAGPLATILNAATSWFIQPTTEFVKIISSRYTAPLEQR
                     INQFYDKLKPKAGDGQQEAKRNEAPNLATVERDGMDFAGMSPQEQESNWSKNLRMWVA
                     VAKTFGQLLRDTHHAGRVLMMIAWHDEQATAMMVETMDSKLINLALESEARLTPYKSA
                     ILSDPTLDMTERKKKLTEFQEHFEQYQNLNERMWMDLSLDENGRHKIAQQIEEHLHQL
                     AQVMTQKDIIKLASIQTQRYGAVRTLVTALALGEVRSFNTAEANRNLEPEARQAQRAI
                     RNGFHMQEYVNKYRDHVQVAMEKMGYENARKKGPSNNCEAIFALGH"
     gene            2154..3350
                     /locus_tag="VIG33_16885"
     CDS             2154..3350
                     /locus_tag="VIG33_16885"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY8272571.1"
                     /translation="MDRSLFKQDPILNGFIFQQKMLRFLFTVYLLISGSAAQAQITCA
                     QIFSNSGPNQPQIVSLSGRAPDIHIGYYLEPHPLQRVQIRNASLKDLLQIGGWLTLPG
                     EPAYLEITDISKNLQSFTARDPLHLRFFTVLEQNGFIEILEISNPHPPTFKKGTGVEN
                     LERKFTWRTRSVSDLTADLFQQQKDVTDLLEERSYEEKENLKAAISYGVDKLKKNKGM
                     TEQDLETLNQTLRLGKDSEHKQFTWPSDVLGVGLIRGFAPRLQSFHLEYQRNQRGGWD
                     QTQGEPSNFLVDLSQTEMTNGSPPLYYLPAKQVPPAIKNWLKEFNKINENTDFFKIAE
                     LYQKFVIIHPFSDGNGRTSRILLDLMLMKAGFRGLKHSETDSRSTIYRTQEEIAEMLA
                     TNIIPS"
     gene            3408..5153
                     /locus_tag="VIG33_16890"
     CDS             3408..5153
                     /locus_tag="VIG33_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272572.1"
                     /translation="MLVRRLKLTARLVGWTLSSLALAQEKSPVIANKHMMPNAVQVQI
                     TPRGLKYFETGLGTLLGTMGYNFSEGYFDAKKIVADKSIDLKELEKKSPEGMKIYKIT
                     KDLLTKWLVGFSLTDHRPVVELGESGYTASFSRFALVTDEALMAQLGKRDGAVLAIDL
                     EVKRLTLATQSVRAWDMNNMPFVKIGMDEALLTAGDDKTSMKIRLPFYIRLNAQGILE
                     FEALEIQENLADIPLSVKYKKLIVPQVSIDVNGHRYVMNTQQLKATINEQLPKALIEV
                     RAYLSEFAHRQLPEMLNQKVKENLAGSLEQVQDLVAPGQEPQDNRPTFKWGLKLTHFQ
                     LKSSLNMGLAAYAEDPLNPHVNLIPGSASRGLPILDDLPLTSYDIGMTLDRGLINRIL
                     QLSFLRNNFEKIPQSDGTSLKLTAPPIIDYIQPPAPTGDKEAYLKLRVSVETEPDKPL
                     FLEKTIVLSFDIVAKIRKSQKGAEGMEIAQQYIDVNSLYMDSKYLTFAGKMAESLGHQ
                     VTKGIKENLELRSKDWIKNNETIPGTLGLPPAFLGMKLDLQKVVMDPTGHLLMYLNYK
                     LNNKLNYKTALGVQK"
     gene            5150..5662
                     /locus_tag="VIG33_16895"
     CDS             5150..5662
                     /locus_tag="VIG33_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272573.1"
                     /translation="MRSLQIFFVNAITLIIGLSLMSGSFSSNKAQAAPLETVPKIQIH
                     KIGNYKGQYLTAFYALGTRPFLSTDSSQVILSEIKAAKTELVTADSLTLPAVDLQKIG
                     FRPPYNLVMIVVSPAPDFTFVNPDSSIIKGMNQSENHRATLIRSFSKVELDSLATGEG
                     SQREFVLTLD"
     gene            complement(5706..8591)
                     /locus_tag="VIG33_16900"
     CDS             complement(5706..8591)
                     /locus_tag="VIG33_16900"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEY8272574.1"
                     /translation="MEQSLIKKIFLYFNLEKMSLAFLVVFSQLILNSTTVLAVAPPPL
                     PGVKPGVGRGGGSAPGLPPGSPPGLPGQQCTPAGNMPVSPDMCQMMPNMCGCERFPSD
                     PKITPNRRRLDPSLRKAFPKVAPGIEHFSQLGYCTGVSSGDTYTASSNGKVSTQGGVR
                     KYTLRLKQAVEPKTGRTYLKTTMDDQADDNDRMKRCSAPSQVEPGVSAKTRPTNAPTL
                     FFVEGETAEITVINESDPAKDNSTSIHWHGIILPNDQDGIVDITQAPIPPGGRKVYRF
                     TLQHNGTYWYHPHDLNEQDTKGAFIIFPNPAREARLNQEFGGVGVKYHQDRVIFLSDY
                     KIRKTMKIFNYLRDEDRNAYELDSGIHRGWLSQLKCGNEYIENFKMMKMFWMDKADVW
                     YNSFFMNDETCLNCGSKTSKIQNLHTEYPGQTFPRLNEFSNIQAGERVRLRLINGSAS
                     SYFFLDYANTSTLAPNQKLDFLVVAKDGLPVKPFYIDQLYMGMGETYDVLVEVPDDGT
                     LYELRAKSIDDIPDKRSVRTLIGHNPLNQGETSSVVPARNVPVQVCGPYDDEEDQTSQ
                     ISYSQLLAPDSRVNGRPADLRPFEVYHSEKPIARYNLSLSGSMEDYHWKISGENGTKL
                     RTDPMHMLYMTIKEGYRIRVTIENDMVMGMMNHPWHLHGNWFRLIKEGESDADIAKKA
                     LLHTATIFPGQKVTLEFYADPAYRGAWMFHCHNLYHMANDMMMYLKYDTVTDEHMSHM
                     TGHHHEKSMLPGALAGIKNQYVVGGVGSWVGTNGYGPSAQFRYRGTLGDNKGWVDLKV
                     DLAGNMSSKNKGLELNSRLRHCFEVNKCVFLDFRLHRSDGSTETSEYIGGQYKPWNSD
                     LMVVEAGAGSICTSDEETKGVRCSPAIKANVSSTIDAGWNTKVTGKVGCEGKYCSEFF
                     ASIQAATTFTPRLTISATCTGSTNAENTGCGLEAKLITDPIALGKQH"
     gene            8858..9334
                     /locus_tag="VIG33_16905"
     CDS             8858..9334
                     /locus_tag="VIG33_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272575.1"
                     /translation="MFQSTLKKFNSTLAGVTSAILFVSFISSQSFALPLSKTLGFFNG
                     ALQAKVSGPKSLTSGSNKDNTFTVQITDANGAPYPAITKEWVKATIEMTSMDMGVDSV
                     RKISDILDAKKQLQGKLAINPEFSMKGPWKLNLSITVSDANGQPMTDTQSLTFNVN"
     gene            complement(9341..10288)
                     /locus_tag="VIG33_16910"
     CDS             complement(9341..10288)
                     /locus_tag="VIG33_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272576.1"
                     /translation="MYIDAYAIIVQKDMDGGDSLHREGMYAFGKKLTGNLSYNHFKNE
                     FYITDPLPLRGPAAENILDRFEVSPGIYVRHPDPKKWYSNPDTTSRDQLMPVIAYCAA
                     YEDYPRLWRLFKAVAQRGLFAQNTIRNGEGEIDKKIPDPMHLNLAQFIRAGGWWTAPF
                     YPLLFAFDSVELIGTVLTTLPLHFQDDHLLPRWRNDNDVDDNNVVVQHLLAAQYKPTP
                     LSELSRYIYSISRTKNLGNTLLGEENAVMGALRWYHRNELGGEGNPEIAELYRPLIEK
                     YFNYQTPQEYVIEHVAEYIARIEGKFTEERLAFGQPLSR"
     gene            complement(10377..10664)
                     /locus_tag="VIG33_16915"
     CDS             complement(10377..10664)
                     /locus_tag="VIG33_16915"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY8272577.1"
                     /translation="MFRAEYMDAVPKLRRLILEGQHEEAKRLAHTLKGVAGNLCALEL
                     SEAAATVENAFHKDKNKLPVMETLIDGLEKALTSVLQAIPSSPSLSSQYET"
     gene            complement(10707..13886)
                     /locus_tag="VIG33_16920"
     CDS             complement(10707..13886)
                     /locus_tag="VIG33_16920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY8272578.1"
                     /translation="MQERKRILLLIMKMMILSSFMIRMTWALPVAPSPKQRVLVIYSY
                     HDTQPWQANIKEALFARLNAAPPEQRPELFEERLDSYRLTAVFDKKFLALLEAKYRDV
                     KLDLVIAENDYAYDFISKNPDFLPNVKRLLIPLTKGKPEEETLTAHENATRGLNTALQ
                     VLPNTERIVVILERFDHSGHVFASYNTKVWEQFKSSQAPLLAKNIQLDIWENFSFAEL
                     YQRAQSLSKNTVILFYPFIQDRLGERRVPRDVVQQLSKLSPAPIFVPHDSLLGSGAVG
                     GYVVSARKFGDLVGQAVLGLPLPRSRTEINAFMMGNYFDDVQLKRWHIPDSVLPANSI
                     IINRKISDWYKYRWEIVSAFLALSFELALIIGLLYNLRLRKEVTRALAQEKDLLDLRV
                     RERTAELERSHALFHTAQNAANIGYYVTDLTTGTWANDALFEDIFGIDSNFARDFEHW
                     CNIIHPDDVQHVVDCFKTTTNKFQSFPVVEYRIIRPSDGEVRWIAAWVHNFYDDKSNT
                     NQQVGLIQDITERKQAEQQLRETAEQLQLVLEGGHLGYWDWNIVTNEVQRNEIWAEMF
                     GYTHEEIQNTTQQWTDFIYPEDRERVRKSVYDVLEGKKPYHEAEYRILHKDGGFRWIL
                     DHAKVVQRDKNGRPMRMCGTQVDISKLKQTEANMREAELKANLANQAKGEFLANMSHE
                     IRTPMNGIIGMADLALKTPLTPKQLNFLNKIKFSAKSLLSIINDILDFSKIESGKIEI
                     ESIRFSLDELLNYVTDITSVAAKEKNIVVTIVVHDKTPKWLIGDSLRLGQILLNLVGN
                     AIKFTEKGKIIISITPAELHSDTIRLGFQVQDSGVGMTPSQMEKLFQPFSQADGGVTR
                     KYGGTGLGLVISKKLVELMGGKIAVESVYGVGSTFTFTVPLGIAQVSLQAPNTPDKLD
                     RQYRSKAHARLMGRKILLVEDNAINRELGIELLSTLGCVTEVAINGREGLRRVLIESF
                     DLVLMDIQMPEMDGLTATRLIRADERFSKLPIVALTANAMTSDKENCLAAGMNDFLTK
                     PIDSAKLAAVLNQWLGHPDPLTPFDIQAL"
     gene            complement(14210..16123)
                     /locus_tag="VIG33_16925"
     CDS             complement(14210..16123)
                     /locus_tag="VIG33_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY8272579.1"
                     /translation="MSKHSAMLKSVFTFFVGLSSMATAQNSGTTNAEVVPGEYIVKFK
                     AMSAPTQALNKVQNKIALKGSFPRMNMYHISLKGGTTEGLDDLAGDQDVEYIEPNYIF
                     RKADFNDQEGLGAPAQVYEAGAVQALDQVSASSTTYSQSGAQTKVSDAWSILSSSVTY
                     RPIVAIIDTGLDKDHPIFVASNALWVNTHEIPNNGIDDDYNGYIDDVNGWNFISNTGD
                     FADDDGHGTHVAGIVVGTSLNIFANPVTETAKMQIMPLKFLDANGSGTTSSAIRALYY
                     AVNNGAKVINNSWGGSSYSRALHEAMTFAYANRVFLATAAGNNGKNNDSVPMYPANYD
                     VPSNVSVAATTDYDYMASFSNFGSRLVHIASPGVLILSTLPGNRNGTMSGTSMATPFV
                     AGMAALAMREAPFLTGYQIKDIMMAKATNVTNLLGKVSTSARINSYNLIAEAKIQASV
                     QAEQPDYSPVYQADSRSAASEPETSHAGCGLISTALLKGPGNKTNDGGFGSAGMMAGL
                     FLLPVIIWAVLRQRAKEKEMEQAADPAGRRRFDRFKMDSDIRVMVGDRELVGSLKTIS
                     LGGASFSADEALEKGGIITMKISSPDGKDMVEVQGQVVWNEANHAYGVQFAGAEQGTL
                     TMIQKWTSGLMKV"
     gene            16296..16916
                     /locus_tag="VIG33_16930"
     CDS             16296..16916
                     /locus_tag="VIG33_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEY8272580.1"
                     /translation="MNQSLKTLRQIFLKGLFTFLPIAVTIYILYAGIIIMESMLGTTV
                     QRLFPSLYVPGVGLILTVVFIFLLGLVLNNLLTASLFSNIERQLMKVPFIKAIYSPLR
                     DVMNLFSQNGSKDLKSVVLVEIGDGNIKALGLVTRDNFKDLGVGQHFNDHVAVFVPFS
                     YGLGGFTLLVHRSKITEIDMPAEKAMSLAITGWVKVAHKPEGEIHE"
     gene            16909..18336
                     /locus_tag="VIG33_16935"
     CDS             16909..18336
                     /locus_tag="VIG33_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272581.1"
                     /translation="MSKATIPPLSMTTLNKAHRINLNTSIYGTFAEIGAGQEVARHFF
                     LAGKASQTVAKTISAYDMTFSDEIYGREKSGRYVCESRLEKMLSHEFSLLEERLSDKR
                     GAGTQFFTFANTVATGSANDKKFCHGWMGIRFQKHPLGGFNDIILHVRMQDRHRLQQQ
                     EALGILGVNLIDAAFYHTDKTDNDNAEEFISSLVDNLKEGQIIIDVLRISGEDLKHFD
                     PHLTNLELVHRGLAEAILFGPNKEILHISDAVYKKAVLLQRGTYRPVTKTHVDVLHKG
                     MSHFKKDFGLTEKQCLPIMELTMRNLQTDGEVNPKDFLDRIETLCSLGHHVLISNFFY
                     FYSLKSFFRQYTQEPLAIVIGASHLDRVFDESGYPDLAGGILEGLGRLLDSHTKLYVY
                     PHKTDLICMTAKTFFPTPSLRNIYAHFKDNQQICDIAGCDEAEVYTHSAQVHEMILQK
                     NPQWETLVPTGVRDLIRNKKLFGCS"
     gene            19162..19563
                     /locus_tag="VIG33_16940"
     CDS             19162..19563
                     /locus_tag="VIG33_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272582.1"
                     /translation="MRQIDFGFLQKQSLSFGGSSLLGKRKTARPLSTKKPLHVVLKSQ
                     GSSLFNPGNRALEKLIRDHAKKYSIAVYAFSFNWSHLHLVLKIPTRGSYLAFIRTVTA
                     AIISFLSKQKGRTLKGTFSLRPFTRIFTAVR"
BASE COUNT         5379 a         4715 c         4178 g         5411 t
ORIGIN      
        1 gatacggcta tcataagtcg cgaaggcgct tattattcat tttgaaactc gtagcgcgac
       61 tttacttctg tttgagtaca accagaagtc caatgaaaac attcggatag aataaagact
      121 tgttttaata aaaaccaata ggcagaggcc tcttttgtgt aaagccaaaa tccgccatcg
      181 taaaatcctt agacatccct ctcttgacca aaacaaatta aacacgcttt cgttgcggca
      241 tgtttgtggc aagattcgga tttaggacgg attcaaatag aggagatgga catgactcgt
      301 ttttatgcag gaagcatcaa acattttggc tatagttttt taaatgcaat attaaccacc
      361 tcactgattc tgggcccctg cgtgggccaa ggtgctggaa ctccagttcc agataatact
      421 caaaatcaaa ccaatacctc ggtggaggcc attattccca ggattgaagg agtcgatctt
      481 gatacagttg aaatcgctcc ggatggcgtg gccgtttccc tgcctgctcc ccttgaaaaa
      541 atagctcagg aacaagcgaa tgctgccaat gccgctccgg catgggttaa agaactgcgc
      601 agtcgcagcg tgggcgaata cttccctcca aatcttgatg ataaaagcga agcttggcgg
      661 caaggaaagc aacgcctgct gacagacgcc caaaatctgc ataaatactt tgaagagcag
      721 gataagtccg gagtttataa agagtttatt gataaaaagc tcagcccttt cgtgaatact
      781 ttggatgcga agaaaatcgc tgcttctatt cgagaccaga ggatcactcg acctattaaa
      841 gaatggacgg tggaaacttt agtagtcact gagcagatcg cttatctggg tcgttatccc
      901 aattttgtta aaagaacgat gagcttattt tctggccgcc atttacgcgg agccagcgcc
      961 gttgccggca ccttggtttt gagtgccgta ggtcttttca cggcttatca ttttggttac
     1021 cattttgatg ccgccacttt gagcgcaggg gcccatgctg tcggtacagg aattaaatct
     1081 gtgacatcgg cagtggacat gaaagcttta tccatgggtg ctttttcgac aggtattgct
     1141 gttggggctt ttctcggacg cgctcttggc ggggctttcc aagcaggacc tctggcaacg
     1201 attctgaatg ccgccaccag ctggtttatt caaccgacca cggaattcgt caaaatcata
     1261 tcttcccgtt atactgcccc ccttgagcaa aggattaatc aattctatga caagctcaag
     1321 cccaaggcag gcgatggaca acaagaagca aaaagaaatg aagcgccgaa cttagccacg
     1381 gttgaaagag acggaatgga ctttgcgggg atgtcccccc aggaacaaga aagtaactgg
     1441 agtaaaaatc tgcgcatgtg ggtcgccgta gcgaagacct tcggtcagct cttgcgcgac
     1501 acccaccatg ccggccgcgt tttaatgatg atcgcttggc atgatgagca ggcgaccgcg
     1561 atgatggttg aaaccatgga ctcaaaactc atcaatcttg ccctggaatc cgaagcccgt
     1621 ttgactcctt ataagtcagc aatcctcagt gatcctactt tagatatgac cgaaagaaaa
     1681 aagaagctca cagagtttca agaacatttt gagcaatatc aaaatttaaa cgaacgcatg
     1741 tggatggact taagcttaga cgagaatggc cgccataaga tagctcaaca gattgaagaa
     1801 catcttcatc aattggcgca agtgatgact caaaaggata ttattaagct ggcttcaatt
     1861 caaactcaac gctatggcgc cgttcgcacg ctggtcactg ccctggctct aggtgaagtt
     1921 cgctctttca ataccgcgga ggccaatcga aatctcgagc ccgaggccag acaggctcag
     1981 agggccattc gcaatggctt ccacatgcaa gaatatgtga ataaataccg ggaccatgta
     2041 caagtcgcga tggaaaaaat gggctacgaa aatgccagaa agaaaggtcc ttctaataac
     2101 tgcgaggcca tcttcgccct ggggcattag gtgattcttg aggaaaaaac tttatggata
     2161 gatctttatt caagcaggac cccattctaa atggcttcat ttttcaacaa aaaatgttac
     2221 gctttttgtt cactgtctac ctattaatca gtgggtccgc tgctcaagcc cagattactt
     2281 gcgctcaaat tttttctaat tcgggtccca atcagcccca aattgtttca ttgagtggac
     2341 gcgcacctga cattcacatt ggttactatc tggaacctca tcctcttcag cgagtgcaaa
     2401 ttcgcaacgc atctttaaaa gatcttctcc aaatcggggg ttggttgaca cttcccggtg
     2461 aaccggctta tctcgaaatc acagatattt caaagaatct gcagagtttc acggcaagag
     2521 atcctcttca tttacgtttt ttcaccgtgc tagaacaaaa tggtttcatt gaaatcttag
     2581 aaatttcgaa cccacatccg ccaactttta aaaaaggaac aggagttgaa aatcttgaac
     2641 gaaaattcac ctggcgaacg cgttccgttt ctgaccttac cgcggatctt tttcaacagc
     2701 aaaaagacgt aacggattta ttggaagaaa gaagttatga agaaaaagag aatcttaaag
     2761 ccgcaatatc ctacggcgtc gataagttaa aaaaaaataa aggaatgacc gagcaggatc
     2821 tagagactct gaatcagact ttaagactcg gaaaagactc tgagcacaaa caattcactt
     2881 ggccaagtga tgttcttgga gttggcttaa tcagaggctt tgcgccacga cttcaaagtt
     2941 ttcatctcga atatcagagg aatcaaaggg gtggttggga tcaaacccaa ggcgaaccat
     3001 ctaatttttt agtggatctt tcgcaaacag aaatgacaaa tggatctccg cctctttact
     3061 atctgccagc caaacaagtt cctccggcta ttaaaaactg gctcaaggaa ttcaacaaga
     3121 taaatgaaaa tacggatttt tttaaaatcg ccgaacttta tcaaaaattt gttattatcc
     3181 atccattttc tgacggcaat ggcaggacct cccgtatttt attggattta atgctcatga
     3241 aagcgggatt cagggggtta aaacattcag aaacggattc ccgatcaact atctacagaa
     3301 ctcaggagga aattgcggaa atgctagcta caaacattat accgtcctga atcgcgatca
     3361 tgttggaatc atatttgcat gcatacatgt ggcgcaagga gcaccgaatg ctagtaagac
     3421 gactaaaatt aaccgcacgc cttgtgggat ggactttgag ttctttagct ttggctcaag
     3481 aaaagtctcc tgttatcgct aacaagcata tgatgcccaa tgcggtccaa gtgcagatca
     3541 cgccaagggg tctgaaatac tttgaaactg ggttgggcac cctcctcggc actatgggct
     3601 ataacttttc tgaaggctac tttgatgcca aaaaaattgt cgccgacaaa tccattgacc
     3661 tcaaagaact tgaaaagaaa agccctgaag gcatgaaaat ttacaagata acgaaggatc
     3721 tgctcaccaa atggctggtg ggtttctctc tgactgatca ccggcctgtg gtggagcttg
     3781 gggaatcggg ctacaccgcc tcgttctcac gtttcgcctt agtgaccgat gaagccctga
     3841 tggctcaact gggtaaacgg gacggtgccg ttttggcgat tgatctggaa gtgaaacggc
     3901 tgaccctggc cacccagtcc gtgcgcgcct gggacatgaa caatatgccc tttgtaaaaa
     3961 tcggtatgga tgaggccctt ctcaccgcag gagatgataa aacgtcgatg aaaatacgac
     4021 tgccctttta tattcgtttg aatgctcagg gaatcctgga gtttgaagcc ttggaaatcc
     4081 aagaaaatct tgctgatatt cccttaagtg taaaatacaa aaaactcatt gtgccccaag
     4141 tgtccatcga tgtgaacggt catcgctatg tgatgaatac gcagcaactc aaagccacta
     4201 ttaatgaaca actgcccaaa gccttgattg aagtgcgcgc gtacttgagc gaattcgctc
     4261 acagacaatt gccagaaatg ctcaaccaaa aggtcaaaga aaacttagcc ggcagcttgg
     4321 agcaagtcca ggacttggtg gcgccaggac aagaacccca ggacaaccgt ccgacattta
     4381 agtgggggct caagctcacc catttccaac tgaagagttc tttgaatatg ggccttgcgg
     4441 cttacgccga agaccctctc aatccccatg tcaatttaat cccaggctcc gcctcacgtg
     4501 gcttacctat tttagatgat ttgccactca caagttatga tatcgggatg accttggatc
     4561 gtggtctcat caatcgcatt ttacagttga gcttcttaag aaataacttt gaaaaaatcc
     4621 ctcagtctga tggaacttct ctgaagttaa cggcaccgcc gattatagat tatatccagc
     4681 cgccagcccc cacaggggat aaggaagctt atctgaagct cagagtcagc gtggaaactg
     4741 agcccgacaa acctctgttc ttggaaaaaa ccatcgtttt atcttttgat atagtggcaa
     4801 aaatacgaaa gtcccagaag ggcgccgagg gcatggagat cgctcagcaa tatattgatg
     4861 tcaactctct gtatatggat tccaagtatt taacctttgc tggaaaaatg gccgaatccc
     4921 tgggccatca agtgacgaaa ggcatcaaag aaaaccttga acttcgtagc aaagactgga
     4981 taaaaaacaa tgaaacgatt ccaggcacgc taggccttcc gcccgcgttc ttagggatga
     5041 aattagattt gcaaaaagtg gtcatggacc caaccggtca tttgcttatg tatttaaatt
     5101 acaaactcaa taataaactt aattataaaa ccgctctagg agtacaaaaa tgagatccct
     5161 acagattttt ttcgtcaatg ccatcactct tatcatcggc ctcagcttga tgagcgggtc
     5221 tttttcctct aataaggcgc aagccgcacc tctggagaca gttccaaaaa tccaaataca
     5281 caaaattggt aactacaaag gacagtatct caccgcattc tatgccttag gcactcgccc
     5341 tttcttgtcg acggactcaa gccaagtcat attgtcagaa attaaagccg ctaagacaga
     5401 attagtgact gcggattccc tcaccctacc ggcggttgac ttgcaaaaaa ttggcttccg
     5461 ccccccttat aacctagtga tgatcgtagt ctctccggct cccgatttta cattcgtaaa
     5521 cccggatagc tcgatcatca aaggaatgaa ccaaagcgag aatcatcgag ccaccttgat
     5581 ccgctccttt tcgaaagtgg aactcgattc cttggcgaca ggtgaaggct cacaaagaga
     5641 attcgttttg accttggatt aaggatctga gggggcttag ccccctgcca ttcctcgata
     5701 aaaaactaat gctgtttccc caaggcgata gggtcggtga tcaacttcgc ctccaagcca
     5761 catcccgtgt tctcggcatt ggtcgatcca gtgcaagtcg cagaaatcgt aaggcgtgga
     5821 gtgaaagtcg tggccgcctg aatgctcgcg aagaattcac tacaatactt tccttcgcaa
     5881 cccaccttgc cagtcacttt tgtattccac cctgcatcga tcgtagaact tacattggct
     5941 tttatcgcag gagaacagcg gacgcctttg gtctcttcat ctgaagtgca gatcgagccc
     6001 gctccggcct caacgaccat cagatctgaa ttccagggct tatactgacc gccgatatat
     6061 tctgaagttt cagtgctgcc gtcggagcga tgaagacgga aatctaaaaa gacgcattta
     6121 ttgacttcaa agcagtgcct caagcggctg ttgagttcaa gacctttgtt cttcgaactc
     6181 atattgcctg caagatccac tttgagatcc acccaacctt tattgtcccc taaggtgcca
     6241 cgatagcgaa actgggcgct gggcccatag ccattggtgc cgacccagct gcccactccg
     6301 ccgacgacgt attgattctt tatacccgct aaagcccctg ggagcataga tttctcgtga
     6361 tggtggcctg tcatatgaga catatgctca tccgtcacgg tgtcatactt caagtacatc
     6421 atcatgtcgt tggccatatg atacagatta tggcagtgga acatccaggc tccccgatag
     6481 gcgggatccg catagaattc aagagtcact ttttggccgg ggaaaatagt ggcggtgtga
     6541 agcagcgctt ttttggcaat atccgcgtcg gattctcctt ccttaatcaa gcggaaccag
     6601 ttgccatgca agtgccaggg atgattcatc atacccatca ccatatcatt ttcgatggtt
     6661 acccgaatgc ggtaaccctc tttaatagtc atgtaaagca tatgcatggg atccgttcgg
     6721 agtttcgttc cattctctcc gctgattttc caatgatagt cttccatgct gccgctcaag
     6781 gataagttgt aacgggcaat gggtttttcg ctgtgataca cttcaaaggg acgtaaatct
     6841 gcgggccgtc cattcactcg ggagtccgga gccagcagtt gactgtaact aatctggctt
     6901 gtttgatctt cctcatcatc atagggaccg cacacctgca caggaacgtt tcgcgccgga
     6961 acgacagagc tggtctctcc ttggttcaac ggattgtgcc cgatcaaagt tcttacagag
     7021 cgcttatctg gaatatcatc aatggatttt gcacgaagct cataaagagt tccgtcgtca
     7081 gggacttcaa ccaatacgtc ataggtctca cccattccca tataaagttg atcaatatag
     7141 aaaggtttta ctggcaagcc gtcctttgcg acgactaaga aatccaattt ttgattcgga
     7201 gccaaagtgc tggtattcgc ataatccaaa aagaagtaag aagatgctga gccgttgatg
     7261 aggcgcagac gcactctctc acccgcttga atattgctga attcattgag ccgtggaaat
     7321 gtttgacctg gatattctgt gtgaagattt tggatttttg aagtcttaga gccacagttc
     7381 aggcaggttt cgtcgttcat aaagaaggag ttgtaccaaa catccgcctt atccatccaa
     7441 aacattttca tcatcttgaa attttcaatg tattcattcc cacatttcaa ctgacttaac
     7501 catcccctat gaattccact gtcaagctca taggcgttgc gatcttcatc gcggaggtaa
     7561 ttaaagattt tcatcgtttt gcgtatctta taatcgctta agaatataac acggtcttgg
     7621 tgatacttca ctcccactcc gccaaattct tgattcagtc gcgcttccct tgcaggattt
     7681 gggaagatga tgaacgcgcc cttggtgtct tgctcattca aatcatgggg gtgataccag
     7741 taggtgccat tgtgctgaag cgtgaaccga taaactttcc gtcctcctgg agggatcggc
     7801 gcttgagtaa tatccacaat tccatcttga tcatttggca aaataattcc atgccagtgg
     7861 atggaggtgc tgttgtcctt tgcaggatca gactcattga tcacggtgat ctccgcggtc
     7921 tcgccttcga caaagaataa ggttggcgca ttggtcgggc gagtcttcgc gctcactcct
     7981 ggctcaactt gagacggagc actgcaacgc ttcattctgt cattgtcgtc cgcttgatca
     8041 tccatcgtcg tcttcaaata agtacggccc gttttcggct cgaccgcctg ctttaagcgc
     8101 aaagtgtatt ttcgcactcc accttgcgtg ctgactttgc cattacttga agcggtgtag
     8161 gtgtctccag agctgactcc ggtgcaatac ccgagttgac tgaagtgctc gatccctggt
     8221 gcgactttag gaaaggcttt tcttaaactg ggatccagac gacggcgatt gggtgtaatt
     8281 ttaggatcgc tagggaacct ttcgcagcca cacatattcg gcatcatttg gcacatatcg
     8341 ggggacacag gcatattccc tgcgggagtg cactgttgac ctggcaaacc aggaggcgaa
     8401 cccggtggca gaccgggtgc ggagccaccc ccacggccta cacctggctt cacaccagga
     8461 agtggcggtg gagcaaccgc caatacggtt gtagaattta aaatcaattg agaaaaaact
     8521 actaaaaaag ccagggacat tttttccaaa ttaaaatata aaaatatttt tttaatcaag
     8581 ctctgctcca tgattcctct ttttgagaaa gtttattcaa tcttttagaa tgcaattata
     8641 gtgtcagttt taaggggctt aggacgatta tagagaagca ccactgtcag gatcctgaca
     8701 gtggtgacca ttcacggcaa cctcaggagg cttatagagg gtcagagagc gcggcgcctt
     8761 atttgacaaa aaaatgcaaa tggaatactc aggagagtat tcatttaatt ttattttaaa
     8821 tttaaatgga atgttcaata aaaaaacagg agcgtttatg tttcaaagta cactgaagaa
     8881 atttaattcc actttagcgg gtgtaacatc tgcgatttta tttgtttctt tcatttcaag
     8941 ccaatcattt gctttgcccc tgagtaaaac tctcggattt tttaatgggg ctcttcaagc
     9001 taaagtttct ggccctaaaa gcctcacttc tggcagtaac aaagacaata cttttactgt
     9061 tcaaatcacg gatgccaatg gagctcctta tcccgccatc actaaagagt gggttaaagc
     9121 cactattgag atgacaagca tggatatggg tgtggattca gtaagaaaga tctctgatat
     9181 tctagatgca aaaaaacagc ttcaagggaa attggctatc aatcctgaat tctcaatgaa
     9241 ggggccttgg aagctcaacc tcagcattac tgtttccgat gccaatggcc aacccatgac
     9301 cgatactcaa tcactgactt tcaacgttaa ctaataaaaa ctatcgcgag agaggctgac
     9361 caaaagccag cctttcctca gtgaactttc cctctatcct agcaatatac tcggccacgt
     9421 gctcaatcac atattcttgt ggcgtctggt aattaaaata tttttcaatg agcggccgat
     9481 aaagctcggc gatttctgga tttccctccc cgcccaattc attgcgatga taccaacgaa
     9541 gcgcgcccat caccgcgttc tcttcgccca gaagagtgtt acctaaattc tttgtcctgc
     9601 tgatcgaata tatatagcga ctgagctctg acaacggtgt gggcttgtat tgtgccgcga
     9661 gcagatgctg gaccaccaca ttgttgtcgt ccacatcatt atcgtttcgc cagcgaggga
     9721 gcaagtgatc atcttgaaag tgcaaaggaa gggttgtcag gaccgttccg ataagctcga
     9781 ccgaatcaaa agcaaaaagc agcggataaa acggtgccgt ccaccatcct cctgccctga
     9841 tgaattgggc gagattcaga tgcatgggat ccggaatttt tttatcgatt tcaccctcgc
     9901 cattccgaat cgtattttgc gcgaataggc ctctttgcgc gacggcctta aacagcctcc
     9961 aaagtcttgg atagtcttca taagccgcgc aataggcgat cactggcatg agctgatccc
    10021 gagaagtggt gtcgggattt gaataccatt tttttggatc gggatgacgg acataaattc
    10081 ccggagaaac ttcaaagcga tcgaggatat tttccgccgc gggtcctcgc aacggcaaag
    10141 gatctgtgat atagaattca tttttaaaat gattgtagct taagtttcct gtgagctttt
    10201 ttccgaaggc gtacattccc tcacggtgga ggctgtcccc gccgtccatg tctttctgta
    10261 ctataatcgc ataagcgtct atatacataa ctcttatgtt acacggcaat cgcatgggag
    10321 ataatagcca tttttatgga aatcaattgg cgctttttga ggctattttt ttgttcttac
    10381 gtttcatatt gagatgacag gcttggcgat gaaggaatcg cttgcagaac agaagttaat
    10441 gctttctcca agccatcgat aagagtctcc attacgggca atttattttt atctttatga
    10501 aaagcatttt cgacagttgc cgccgcctca gacaattcca gcgcacataa gtttccagcc
    10561 acccccttaa gagtatgcgc cagccttttc gcttcctcat gttgcccttc cagtataagc
    10621 cgtcttaatt ttggcacagc atccatatat tcggctcgaa acatcactag aagtttacgc
    10681 gctaatttag catccccaca caacgcctac aatgcctgaa tatcaaaggg cgtcagcgga
    10741 tctgggtgac ccaaccactg atttaaaacc gccgccaact tggcggagtc gatcggtttg
    10801 gtaagaaaat cattcatgcc tgccgctaag caattttctt tatcactggt catcgcattc
    10861 gccgttaagg caacaatcgg tagcttggaa aaccgctcat ccgcacgaat taatcgggtc
    10921 gcggttaaac catccatttc tggcatttga atatccatca acaccaaatc aaacgactcg
    10981 attaaaacgc gtcgcagccc ttctcgacca tttattgcta cttcagttac gcacccaagc
    11041 gttgacagca actcaatccc caactcgcgg ttaatggcat tatcttcaac caataaaatt
    11101 tttcgcccca taagccgggc atgggccttt gatctatatt gcctatcgag tttatcagga
    11161 gtgttaggag cctgtaatga aacctgagct atgcccaagg gcacggtaaa agtaaaggtg
    11221 ctacctactc catacacact ttccactgca atcttcccac ccatcaattc caccaatttt
    11281 ttgctgataa ctaagcctaa acccgtgccg ccatatttcc gcgtgacgcc accgtccgct
    11341 tgagagaaag gttgaaatag tttttccatt tgactgggag tcatcccaac cccactgtcc
    11401 tgcacctgaa aacctaagcg tattgtatct gaatgcaact ctgcgggggt tatagaaata
    11461 ataatttttc ctttctcggt gaactttatc gcattgccga ccaaattaag caggatttgt
    11521 cctagcctta gcgagtcacc gatgagccat tttggtgttt tatcatggac cacaattgtc
    11581 accacaatat ttttttcttt agctgccaca ctcgttatgt cagtcacata gttaagcaat
    11641 tcatccaatg aaaagcggat cgactctatt tctatcttgc cagattcaat ttttgaaaaa
    11701 tccaggatgt cgttaatgat actaagaagt gatttggcgg aaaattttat tttattcaga
    11761 aaattgagct gttttggggt taatggggtt ttcaaagcca agtccgccat gcctataatg
    11821 ccgttcatcg gggtacgaat ttcatggctc atattcgcca gaaattcccc ttttgcttga
    11881 ttggcaagat tagcttttaa ttcagcttcg cgcatattgg cttctgtctg tttcaatttg
    11941 ctaatatcga cttgcgttcc acacatccgc ataggtctgc catttttatc tcgttgcaca
    12001 acctttgcat ggtcaagaat ccagcgaaaa ccgccatctt tatgcagaat ccgatattct
    12061 gcttcgtgat aaggtttttt tccttctaaa acatcataga cagatttccg tactctttcg
    12121 cgatcctccg ggtaaataaa atccgtccat tgttgcgtag tattctgaat ttcttcatga
    12181 gtatatccga acatttcagc ccaaatttcg ttgcgctgga cttcattggt gacaatattc
    12241 caatcccaat aaccgagatg ccccccttcc aatactaatt gcagttgttc tgcggtttca
    12301 cgcaattgtt gctctgcttg tttgcgctcg gtaatgtcct gaatcaagcc gacttgctga
    12361 ttcgtattgc tcttgtcatc ataaaaatta tgcacccatg cagctatcca acggacttct
    12421 ccatcacttg gccttataat tctatattcg acaacaggaa atgactggaa cttattggtt
    12481 gtcgttttaa agcaatccac aacgtgttga acatcatcag gatgaataat gttgcaccaa
    12541 tgctcaaaat cccgggcaaa attactatca atgccaaaaa tgtcttcaaa gagagcgtca
    12601 tttgcccatg ttcccgtagt taaatcagtg acataatatc caatattcgc cgcattttgg
    12661 gcggtatgaa acaaagcatg acttctctcc agttcggcag tgcgttctct cactcgcaaa
    12721 tcgagtaaat ctttctcttg agctaaagca cgagtcactt ctttacgtaa gcgcaaattg
    12781 tacaataacc ctatgatcaa cgccaattca aaactcaagg cgagaaatgc ggagacaatt
    12841 tcccaacgat atttgtacca gtctgatatc ttgcgattaa taataatact attggcagga
    12901 agaacgctat cagggatgtg ccaacgtttt agttgaacat catcaaaata attccccatc
    12961 ataaaggcat ttatttctgt gcggcttctg ggtaacggca aaccgagcac cgcctgcccg
    13021 accaaatccc caaattttct cgcacttacc acataaccgc cgaccgcgcc tgaacctagc
    13081 aaactatcat gaggcacaaa aatcggcgcg ggagaaagct tgcttaactg ctgaaccaca
    13141 tcacgaggaa ctcggcgctc gcctaaacga tcttgtatga atggataaaa taatatcact
    13201 gtgttttttg aaagactttg agcccgttga taaagttccg caaaactaaa attttcccaa
    13261 atatccaatt gaatgttttt agccaatagc ggtgcttggc tgcttttaaa ttgttcccaa
    13321 actttagtat tatacgatgc aaaaacatgt ccactatggt caaaacgttc cagaatgaca
    13381 acaatgcgct ctgtattggg cagcacttgt aatgcggtat taagccccct ggtcgcattt
    13441 tcatgcgccg ttaaagtttc ttcttccggt tttcctttgg ttaaaggaat caagagccgt
    13501 tttacattcg gcaaaaaatc aggattttta ctgataaaat catacgcata atcattttct
    13561 gcaatcacta aatccagctt tacatctcgg tatttagcct ccaatagggc taaaaacttt
    13621 ttgtcaaaaa ctgcagttaa tcgatatgaa tctaaacgct cttcaaataa ttcagggcgt
    13681 tgttcagggg gagccgcgtt taagcgagcg aaaagggctt ctttaatgtt ggcttgccac
    13741 ggttgggtgt cgtgataaga atatatgacc agtacacgct gtttaggcga gggcgcaacg
    13801 ggcaaggccc aagtcatcct tatcatgaaa gaagataaaa tcatcatttt cataatcagc
    13861 aataaaattc tttttctttc ttgcattata ttttctgtat aaacctcata aatagaaaat
    13921 ttatggttga gctttctttt acttaagact ccacaagttg aggactcgta gcttggcctt
    13981 gggaaactga tcgaagattt aaattggcat acaggcttcc tgcctactta taacatatat
    14041 ttaactgcac tattgctgga attgatagag ggatcttttt attcttgagc ggcatagccc
    14101 aagctcacgt aaagaagagt ctccttacaa gggaaaataa aaagcggggt ctttatcgac
    14161 cccgcttttt attttcatct tatcgttttt tctacattaa catctcaaac taaaccttca
    14221 tcaaacctga tgtccatttt tggatcatgg tgagagttcc ctgctcggcg ccggcgaact
    14281 gcactccgta ggcgtggttg gcttcgttcc atacaacctg accttggact tcgaccatgt
    14341 ctttgccgtc tgggctggaa atcttcatgg tgataattcc acctttttcg agcgcttcgt
    14401 ccgcactgaa cgatgctcca ccgagagaga ttgttttgag cgatccgacc agttcccggt
    14461 ctccgaccat gactctgata tcggaatcca ttttgaatcg atcgaatctt ctgcgacccg
    14521 ctggatcggc agcctgttcc atttcttttt ctttggcgcg ctgtctgagg acggcccaaa
    14581 taatcacggg taatagaaat aaccccgcca tcatccccgc actgccaaag ccaccgtcat
    14641 tggtcttatt gcctgggccc ttcaaaagag ccgtggaaat cagaccgcaa cctgcatgac
    14701 ttgtttcggg ctccgatgcc gcagaccgag aatccgcttg atagacaggg gaataatcgg
    14761 gctgttccgc ctgcactgag gcttggattt tcgcctccgc aataagattg taagaattaa
    14821 tccgggctga agtggaaact tttcccagca aattggttac gtttgtggct ttcgccatca
    14881 tgatatcttt aatttggtag cccgtaagaa aaggcgcttc acgcatggct agtgccgcca
    14941 tcccggccac gaaaggagtg gccatactcg ttcctgacat cgttccgttg cgattccctg
    15001 gaagagtgct taaaatcaaa actccagggc tggcaatgtg caccaggcga gaaccaaaat
    15061 tcgaaaagct cgccatataa tcatagtccg tggtcgccgc gacggagacg ttactgggaa
    15121 catcgtaatt tgccggatac attggcacac tgtcattgtt cttaccattg ttgcctgccg
    15181 cagtcgccaa gaaaacgcga ttggcatagg caaaggtcat cgcttcatga agggctcgac
    15241 tataggaaga accaccccac gagttgttga ttactttcgc tccgttattc acagcatagt
    15301 aaagggcccg aatcgcactg gaagtcgtac ctgagccatt agcgtccagg aacttcaaag
    15361 gcataatctg catttttgca gtttcagtca caggatttgc aaagatattc aagcttgtac
    15421 ccacgacaat accggcgaca tgagttccgt gaccatcgtc atcagcgaag tctccggtat
    15481 tcgaaataaa attccagcca ttcacatcat caatataacc attataatca tcatcgattc
    15541 cgttattggg gatctcatga gtattgaccc acaaggcatt ggaagcgacg aagatagggt
    15601 gatctttatc aagacccgtg tctataatgg ccacaatagg tcgataggta acggaactcg
    15661 acaaaatact ccatgcatca gaaacttttg tctgagctcc agattgagag taagtcgtcg
    15721 aagaggcgga aacttgatcc aaggcctgaa cagctcctgc ttcataaact tgagccggag
    15781 cgcctaaacc ttcttgatcg ttaaaatccg cttttctaaa aatatagttt ggctcaatgt
    15841 attcaacatc ctgatcgcca gccaaatcat ccaatccctc ggtggttcca ccctttaagg
    15901 aaatatgata catattcatg cgcgggaacg atcctttgag ggcaatctta ttctgcacct
    15961 tattaagggc ctgtgtcggc gctgacatcg ctttaaactt cacgatgtac tcacccggaa
    16021 caacctccgc gtttgttgtg ccgctgtttt gggcagtggc catagaagat aggccgacaa
    16081 aaaacgtaaa aaccgattta agcattgcag aatgtttact cactcatgct cccttgatgg
    16141 ataaaatatt tatcggacaa tggtcttgaa aggtttagag ggcagattga cctaatttgg
    16201 gagaattcca taaaatgccc aaatgtttat cgggctctgc gccttattcg ctttgagctg
    16261 gtgttcaaaa tgagattccc ttagaatgtc tccatatgaa tcaatcactc aaaacactcc
    16321 gtcagatttt tcttaagggc cttttcacct tcctcccgat tgcagtcacc atttacattc
    16381 tgtacgccgg gatcatcatt atggagagta tgctgggaac cactgtgcaa aggctcttcc
    16441 cgagcctcta tgtgcccggt gtcggcttga tattaaccgt ggtctttatt tttttgctgg
    16501 gactggttct gaacaacctc ctgaccgcca gcctctttag taatatcgaa agacagttga
    16561 tgaaggtgcc ttttatcaag gccatttact ctcccctgcg cgatgtgatg aacttgtttt
    16621 cccaaaatgg atccaaagat ttaaaaagcg tggtcttggt ggaaatcggt gatggtaata
    16681 tcaaagctct ggggcttgtg acccgtgaca acttcaaaga cctcggagtt ggccagcact
    16741 ttaatgatca cgtcgctgtt tttgtcccct tcagttatgg acttggaggg tttactttgc
    16801 tcgttcatcg ctctaaaatc actgagattg atatgccagc cgagaaagcc atgagcctgg
    16861 ccatcactgg ctgggtgaaa gtagcccata agccggaggg ggaaattcat gagtaaagcc
    16921 accatccccc ccctttcaat gaccactctg aataaagctc atcgcatcaa tctcaatacc
    16981 tctatctatg gcacctttgc cgaaattggc gcgggccaag aggttgccag gcattttttc
    17041 ttggccggca aagcttccca aactgttgcc aagaccatct ctgcctatga catgactttc
    17101 agtgatgaaa tctatggtcg tgaaaaaagc ggccgttatg tctgcgaatc acggctcgaa
    17161 aaaatgctga gccacgagtt ttcccttctg gaagaacgtc tgagtgacaa acgaggagcc
    17221 ggcacgcagt tcttcacttt tgccaatacc gttgccacgg gctcggccaa tgataaaaaa
    17281 ttctgccacg gttggatggg aattcgtttt caaaaacatc ccctcggcgg ctttaatgat
    17341 atcatcctgc atgtgcgcat gcaggatcgt catcgcctgc aacaacaaga ggccctgggt
    17401 attttgggtg tgaatctcat tgatgcggcc ttctatcata cggataaaac tgacaacgac
    17461 aatgcagaag agtttatctc ttccctcgtc gacaacctca aagagggaca aatcatcatc
    17521 gatgtattgc gcatctccgg cgaagatttg aagcactttg accctcacct caccaattta
    17581 gaactcgtgc atagaggcct tgcggaagcg attctttttg gtccgaacaa agaaattctg
    17641 cacatctcag acgccgttta taaaaaggcc gtgctcttgc aaagagggac ctatcgtccg
    17701 gtgaccaaaa ctcacgtgga tgttctgcat aaaggcatga gccacttcaa aaaggatttc
    17761 ggtctcaccg agaagcagtg cttgcccatc atggaactca ccatgagaaa cctgcaaact
    17821 gatggagagg tgaaccccaa ggatttcttg gatcgcattg aaaccttgtg cagtctcggt
    17881 caccatgttt tgatttcaaa cttcttttac ttctattcct tgaagagttt ctttcgtcag
    17941 tacacccagg agcctctggc gatcgtcatt ggagcctcgc atcttgatcg agtctttgat
    18001 gaaagcggtt acccagatct tgcgggtggc atcctggaag gcctgggcag acttcttgat
    18061 agccatacca aactttacgt ttatcctcat aagaccgact tgatctgcat gaccgcaaag
    18121 actttctttc cgactccgag cctaaggaat atctatgctc attttaagga caatcaacaa
    18181 atttgtgata ttgcgggctg tgatgaagcc gaggtttaca ctcattcggc tcaagttcac
    18241 gaaatgattc tgcaaaaaaa tccccagtgg gaaaccctcg ttccaactgg ggtccgagat
    18301 cttattcgta acaagaagtt atttggctgc tcttaactaa acttttaact taaaaaaccg
    18361 ggtccgtgct aagcctaact gtactaaaat ctttgatctc atgacctcct tccgttggac
    18421 agtcaaaggt tgaagattca actctagaac ctaagatcta gtatttaccg cctagaaatt
    18481 gagaatctag agattaacaa actaggtttt ttacagccta gttactgagg tctgtttcaa
    18541 acttcgtacc gggctcagaa gcaaatcatc ggctttccac ggtcataggt ttgacaactg
    18601 taaatattta attgaacact gggcctcttt cttctttttg tggggatagg ggtttgagca
    18661 gacttgaggt cagacatcca ttgcaaggca aaagctcctt aaattgcttt cataatcact
    18721 ataactcctt ttttacccga tactttctcc catactttta ccggacttct gattgtacgc
    18781 aatcagaagt aaagtcgcgc ccccattttc tcttcgtatt ccaaaatcct tcgcgactta
    18841 tgcaaaccag acatttcttt tcctctttcc cttaatccat tcctggatcc tcttcatcga
    18901 aagagcctct gaagctttaa tccccccccc ccctcaaaaa aattcttctc caagaaaaaa
    18961 caagttgacg gcactttccc ctctcaagaa ttcaatacaa ataacttttt tccctctcac
    19021 cccaaaaact ttaactcacc ccaagatctc ttcagggcga aggtcctccg ttgcataaac
    19081 aggaagccgc ataacactcc cacactccct taaaatctcc tcctagaagc tatggtataa
    19141 agtttttttc ggaggtgtcc tttgcgacaa attgattttg gttttttgca aaaacaaagc
    19201 ttaagctttg gcggaagttc ccttttggga aaaagaaaaa ctgcccggcc cctttccacg
    19261 aaaaaaccac tgcatgtggt gctgaaaagc cagggctctt ctttatttaa ccctggcaac
    19321 cgagccctgg aaaaactaat cagagatcac gctaaaaagt attccattgc tgtttacgct
    19381 ttttccttca actggagtca tcttcattta gttttaaaaa ttcccacccg aggaagttat
    19441 ctggctttta tcagaaccgt cactgcagcc attatttctt ttctctcaaa acaaaaggga
    19501 agaactctta agggaacttt ctcccttcgt ccttttacgc ggatcttcac tgctgtccgg
    19561 taaaaaatta aattttcata ggcgtcaaac gatgatctag gggttgtttc cagcggcaac
    19621 catcgggagg attgctcagg gcaatgcttt gtctcaatac gccaacacgt atttctcgta
    19681 aaa
//
LOCUS       DATBTE010000445         4556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_197614, whole genome shotgun sequence.
ACCESSION   DATBTE010000445 DATBTE010000000
VERSION     DATBTE010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197614"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="VIG33_16945"
     CDS             complement(<1..1155)
                     /locus_tag="VIG33_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY8272583.1"
                     /translation="MKMMGQILALILVAGVYTTAMSATPNENAPQGGVFNLNLGGEPP
                     TLHPIASTDLYAQGVQSYVMDSMLAHDTETWAWKSRIAEKWEVSKDNKTFTFHLRKNV
                     VFHDGKPLTAEDVKFSFDAIFEPAYQAAHLRPYYEGIAKVEVVDPYTVKVTAKDTYFK
                     NFDTIAGMTILPKHIYGDVEKSKKMTRTLIGCGPYSLEKFERGQIITLKKFDKWYGKD
                     DKAWKGMYNFASINMRFYKEEAVILERAKKGDLDYIDLRAESYVKKTEGAPWGKTVLK
                     HKVENNAPKSYGFVGWNLRRELFQDRTVRVALAHMMNREEMNKKFRFGMADLATGPTY
                     VKSEYAPKTVKPILFDPKEATELLAKAGWKDTDKDGTLDKVANGKKTSFKF"
     gene            1340..2392
                     /locus_tag="VIG33_16950"
     CDS             1340..2392
                     /locus_tag="VIG33_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HEY8272584.1"
                     /translation="MNQPTAKFDPTTTISSEFGIFGIPMSEEESLVTLIPVPWEVTTS
                     YGHGASEGPRIIREASEQVDLFDLETGKAYEAGYFMRELPTELKNLNTKYKEKAQELI
                     HLRTSQSNDQAHMDELAKEVNAACEKMTQWVYDQCTSVLENKKLLGLVGGDHSTPWGA
                     IQAVAEKYKGEFGVLHIDAHADLRKAYQGFTQSHASIMYNVMTGDKRPQKLVQLGIRD
                     FCEEEFNFIQSRKDIHTFFDLDLKRRLFNGESWARVCQDILKELPQKVYISFDIDGLD
                     PAFCPNTGTPVPGGLSIDQIFFLFNEIHQSGRKIVAFDLNEVSTGGQEMAEWDGNVGA
                     RILYKLCGWLVKSSQG"
     gene            complement(2396..2773)
                     /locus_tag="VIG33_16955"
     CDS             complement(2396..2773)
                     /locus_tag="VIG33_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEY8272585.1"
                     /translation="MAHSSQVSEKLVSECRQKLIQAKMDILNRVKESRLNLDNDDKGG
                     DEADQTVRVLAEAEFLNMHERLRGQLFEIESALGRIENGSFGLCEETEELIEPERLRA
                     IPWTRLSIEGAEIRESMNKRYAR"
     gene            complement(3130..3495)
                     /locus_tag="VIG33_16960"
     CDS             complement(3130..3495)
                     /locus_tag="VIG33_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272586.1"
                     /translation="MSKFLGFYLIVTVWASLGLASANCVSVIDDLKAMQKAHQSISAS
                     LIGNHESFASILEDYSNTLSASANLHQPVTKEAVTNMNESAKAFRVRGQNAQKLNVKL
                     NTASDQVIAKAIECLKFRK"
     gene            complement(3624..4232)
                     /locus_tag="VIG33_16965"
     CDS             complement(3624..4232)
                     /locus_tag="VIG33_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY8272587.1"
                     /translation="MKTKERILLTSVDLFNRSGVVAVTTNHIAKAMEISPGNLYFHYD
                     NKEEIIGELFKRMCKETYQLWRFRKGNSKNPLTFIDENFELYWKYRFFHREMYSLRRK
                     DPNLAKMWRLHLQKMMKLMDIIYRRWVREGKMAEIKAPREMEYVSESLLAMATTFLQF
                     FESAEKTPGKRGIERGKRHVARLLLSYTVGECKDEFENFIKS"
BASE COUNT         1316 a         1023 c          878 g         1339 t
ORIGIN      
        1 aaatttgaaa gaagtctttt taccattagc cactttatct aaagtgccat ctttatctgt
       61 atctttccaa cccgccttcg ctaatagctc cgtcgcttct tttggatcaa agagaattgg
      121 ttttacagtt ttaggcgcgt attcgctctt cacataagta ggacctgttg caagatcagc
      181 cataccgaaa cggaacttct tattcatttc ttcacgattc atcatatgtg ccaaagccac
      241 gcgaactgtt ctgtcttgga agagttcacg acgaagattc caacctacga aaccataaga
      301 cttaggcgca ttgttctcaa ctttatgttt aagaacggtt tttccccaag gggccccttc
      361 agtttttttc acataagatt cagcgcgaag atcgatgtaa tccaggtcac cttttttagc
      421 acgctcaagg ataacagctt cttctttata aaaacgcata ttgatagaag cgaagttata
      481 catgcctttc cacgctttat cgtctttgcc ataccatttg tcgaatttct ttaaagtgat
      541 gatttggccg cgctcaaatt tttccaaaga ataaggaccg cacccaataa gggtacgagt
      601 cattttcttg gatttttcaa cgtcaccata gatgtgcttt ggaagaatcg tcatgccggc
      661 aatggtatcg aagtttttga agtaagtgtc tttcgcagtc actttcaccg tgtagggatc
      721 aaccacttct acttttgcga tgccctcata gtaaggacgc aagtgggctg cttgataagc
      781 gggttcaaag atagcatcaa aagagaactt tacgtcttcc gccgttaaag gcttgccatc
      841 atgaaacacc acgtttttgc gaagatgaaa agtaaaggtt ttattatctt tagagacttc
      901 ccatttctca gcaatgcgag atttccaggc ccaggtttct gtatcatgcg ccaacataga
      961 atccatgaca tagctttgaa caccctgcgc atacaaatct gtacttgcaa tgggatgcag
     1021 tgttggaggt tccccgccaa gatttaaatt aaagacaccg ccttgaggtg cattttcgtt
     1081 gggggtcgcg gacattgctg tcgtgtaaac tccagctact aaaataaggg ccaaaatctg
     1141 tcccatcatt ttcataatca tctcctccaa aggatttatt agggacctat tcggacccta
     1201 aagtggacct aaaatcaggg aaagcgataa aataatcaag ccattgctca attaatgctc
     1261 cgcaaaatgg caagcgtgaa atgttttctt ttcattaaaa tcagaccata aagctgactc
     1321 agtctctgaa ggagaattca tgaatcaacc tacagccaaa ttcgacccca ccaccactat
     1381 ttcctctgag tttggtatct ttgggatacc aatgagtgaa gaagaatctc tagtcacttt
     1441 gatcccagtc ccctgggaag tcaccacttc ttatggccat ggcgcctctg aaggtcctcg
     1501 cattatccgt gaggccagcg aacaagttga tctcttcgat ttggaaactg ggaaagccta
     1561 cgaagcaggt tattttatgc gcgaactccc aacggaactt aaaaacctaa acactaagta
     1621 caaagagaaa gctcaagaac tgattcattt acgcacctct caaagcaatg atcaggctca
     1681 tatggatgaa cttgctaaag aggtcaatgc ggcttgtgaa aagatgaccc agtgggttta
     1741 tgatcaatgc acttctgttt tagaaaacaa aaagctattg ggacttgtag gcggtgacca
     1801 ctccactcct tggggcgcga tccaagctgt ggctgagaaa tataaaggag aattcggtgt
     1861 tttacatatc gatgcccatg cagatctgcg aaaagcttac caaggtttta ctcagtccca
     1921 tgcctccatt atgtataatg tgatgaccgg cgataaacgc cctcagaaac ttgtacaact
     1981 cggaatccgc gatttctgcg aagaagagtt caacttcatt cagagccgta aagacatcca
     2041 tactttcttt gatttagatt tgaaacgacg tttgttcaat ggagaaagct gggctcgagt
     2101 gtgccaagat attttaaaag aactccccca gaaagtttac atctcttttg atatcgacgg
     2161 tcttgatccc gcattttgcc caaatacagg aaccccagtt ccaggcggtc taagcattga
     2221 tcaaatcttc tttttgttta atgaaattca ccaaagtggt cgaaaaatcg tagcctttga
     2281 tttaaacgaa gtatccacgg gcggccaaga gatggctgaa tgggacggca atgttggcgc
     2341 gcgaattctt tataaacttt gtggctggtt ggtaaaatcc agccaaggct aaagattacc
     2401 tggcgtaacg cttattcatc gactcgcgaa tctccgcgcc ttcgatgctt aagcgagtcc
     2461 aaggaatcgc ccgaagtctt tctggctcaa ttaactcttc cgtctcttca caaagaccaa
     2521 agctgccatt ttcgatgcgc ccaagggcgc tctcaatttc gaaaagttgt ccgcgcagtc
     2581 tttcatgcat atttaagaat tccgcctctg ccaggacacg cacagtttga tccgcttcat
     2641 ctccgccttt atcatcgtta tccaaattca aacgagattc cttaactcgg ttaagaatat
     2701 ccattttcgc ttgaatcaat ttttgtctgc actcactcac cagtttttct gatacctgag
     2761 atgaatgtgc catttttacc ccctcgatta tttcgtaatt tcgaagtgcc aatttggaac
     2821 tgatctatta atcactgccc atgaattttt tgcaagaaaa aatcttaaat taacttaaaa
     2881 ataaataact cactgattca agacagttct tggcgattta atattctatt ggaggctgag
     2941 ctcaatttcc ttataaattt gcttcgtgtc ataaagctgg actaaaaact aaaattgact
     3001 tttaaaacct attctattgg atttggtttc tcattttgag aaaaaaggaa atctccggcg
     3061 gttatggggg aggattatac aggaacaccc caaagttcct gaccgccgga gacatgaaag
     3121 gaaaagaaat cactttcgga attttaaaca ctcaatggct tttgcgatca cttgatctga
     3181 agccgtattc aatttcacat tgagtttctg agcattctga ccacgaactc gaaaggcttt
     3241 ggcactttcg ttcatattag tcaccgcttc tttggtcact ggctgatgaa gattcgcact
     3301 ggcgctgaga gtattggaat aatcctctaa gatagaggca aaactctcgt gattacctat
     3361 aagacttgca gagatacttt gatgggcttt ttgcatagcc ttgagatcat caatcacgct
     3421 gacacaatta gctgaagcca atcctaaaga ggcccaaacc gtaacaataa gataaaatcc
     3481 aagaaactta ctcatgagtg atgactcctt aaatactaag cccaagggac taagtcagga
     3541 ctaaagcaag gttcctgcca tctgtgaagg agttcaaact catctcaaat agattataag
     3601 tggattacag aggccctttt ttgttaagac tttataaaat tttcaaactc gtctttgcat
     3661 tcgccaactg tataagacaa aagcaagcga gccacatgac gttttcctcg ctcgatgcct
     3721 cgcttaccag gagtcttctc ggcgctttca aaaaattgca gaaaagtggt cgccatcgcc
     3781 agcaaagact cagagacata ttccatttcc cttggcgctt tgatttcggc cattttgcct
     3841 tcacgcaccc agcgacgata aataatatcc attaatttca tcattttttg caaatgcaaa
     3901 cgccacattt ttgcaagatt cggatctttg cgccggagag aatacatctc acgatgaaaa
     3961 aaacgatatt tccaatacaa ttcaaaattc tcatcaataa acgtgagggg atttttacta
     4021 ttgcccttgc gaaatctcca taactgataa gtctctttgc acatacgttt aaaaagctcg
     4081 ccaatgattt cttctttatt gtcatagtga aaatataaat tcccaggact aatctccatc
     4141 gccttagcga tatgattagt ggtgacggca acaacaccgc tgcgattaaa tagatctacg
     4201 gaagtcagaa gaatgcgctc tttagttttc attggtattt ctgatattat ttttttatca
     4261 gagtgtcact tgatttcgaa tctgcctcga atctgctctt actagacctt agtctcatct
     4321 ctttctaagg cagaaatggc tccaggcctt gtttgcccgg gagtcttaat gttaaactga
     4381 ttcgatgaga ctacaaaaac attctaaatt aaagtctaag tcgttaaaat tactattaat
     4441 tcctctcgcg ctgatgcttt ctgcgtttct cttttttaag accactccca ctgaagaggt
     4501 ggtccgcgaa gagaaagtga agagtcaact cgagcgccgc accaatattt ccaaag
//
LOCUS       DATBTE010000446         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1768487, whole genome shotgun sequence.
ACCESSION   DATBTE010000446 DATBTE010000000
VERSION     DATBTE010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768487"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1630)
                     /locus_tag="VIG33_16970"
     CDS             complement(<1..1630)
                     /locus_tag="VIG33_16970"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEY8272588.1"
                     /translation="MNNVRTLSGSGERLSFFKLFSEKKFKVEIPIIQRDYAQGRPSEH
                     EVRESFLDALYAYLEQGKPHRDLDFVYGSLLDDEGSYRFVPLDGQQRLTTLFLLHWYL
                     AQISGQTEVLRSVLCKNDKSQFSYETRTSSSEFCDALMACDMDMSNLLEANLDKNNRL
                     SKTIQDKGWFYLSWTHDPTIQSMLTMLDSIHAKFSENPEFFHKLIDEERPVITFLFLN
                     LHAFNLTDDLYIKMNARGKPLTAFENFKAKFEQRIKSFRTELPHYVLDFSGKPVDGYE
                     YFIHKIDTDWADIFWAYRNTLTDDELMNFISLEIANYYLLEEGKKSGTQPTNIDKFFG
                     VGGKVKRLSFQEYCDLGCFSQGFVEHLIQMLDLLHNKGLKDNKLKPYLGSNYYYSEDE
                     VFKKVIVNSTSYQEKLRFYAFYNYMANGRPEAGLVEWLRVVYNLTENTIINTAEDYRR
                     VLISLGELINKNESILDVLMNGCDVAAFSGAQVLEEKVKAHLIRKSKEWKELVVNAEQ
                     HPFFRGQIGFALSFSGILDFYRNKDCCEWDMSQDT"
     gene            complement(1623..>2554)
                     /locus_tag="VIG33_16975"
     CDS             complement(1623..>2554)
                     /locus_tag="VIG33_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272589.1"
                     /translation="TFHEFNAAFKASKEANGQVDIDSLWKEVKDYFLTFDEWYRDRDL
                     YHLIGFLVDCGHSVRDLKSESEKNDPTKTDFKAYLKKEIRKEVNCQLDKLSYGDKELR
                     KVLLLFNIQTLLSTKEADVRFPFDRYKKENWDIEHIRSQTDKEITGALRREWAKDVLE
                     YFADTRDHTVVAIDGVSDDIGEFIIDLRKLLNADKIDDSSFKSLYEKIASYFKEDEVF
                     ELDGVGNLALLDATTNRSYKNAMFPIKRRRIIHNDMNGVFVPICTKNVFMKFYSKKLG
                     EVMYWKESDADEYLNAIKGILQEYLPDQKGAYE"
BASE COUNT          789 a          570 c          406 g          789 t
ORIGIN      
        1 cagtgtcttg gctcatatcc cattcacaac aatctttatt acgatagaaa tccaatatac
       61 cggaaaagct caaggcaaac ccaatttgcc ccctgaaaaa cggatgttgc tcggcattaa
      121 caacaagctc cttccattcc tttgattttc ttattaggtg agccttaact ttttcttcaa
      181 gaacctgagc gccactaaac gctgcaacat cacacccatt catgagtaca tctaatatgg
      241 attcattttt attaattaac tcaccaagag agataaggac tcttctataa tcttcagccg
      301 tattaataat ggtgttttct gtgaggttat aaacaacgcg aagccattct accaagccag
      361 cttcagggcg cccgttcgcc atgtaattat agaaagcgta aaagcgtaat ttttcttggt
      421 agcttgtact atttacaata acttttttaa aaacttcatc ttcagaataa tagtaattag
      481 atccgagata aggtttaagt ttattgtctt tcaatccctt attatgtagt aggtccaaca
      541 tctgaattag gtgctcaaca aagccttgag agaaacaccc caaatcacag tattcctgaa
      601 aagagagacg tttaactttg ccgcctactc cgaagaactt atcgatgtta gttggctgag
      661 tgcctgattt tttaccttct tctaataaat aataattcgc aatctctaag ctaatgaagt
      721 tcatcaactc atcgtcagtt aacgtgttgc gataagccca aaaaatatcc gcccaatctg
      781 tatctatttt atgaatgaaa tactcataac catctactgg cttgccacta aaatctaata
      841 catagtgagg caattcagtc ctaaaggact ttatccgttg ttcgaatttt gccttaaagt
      901 tttcaaaagc agtaagtggc tttccacgcg cattcatctt tatgtataaa tcatcggtta
      961 ggttgaaggc atgcaaattc aaaaacaaaa aggttattac aggccgctcc tcatcaatca
     1021 atttatggaa gaattcagga ttctctgaaa atttagcatg tatcgaatca agcatcgtta
     1081 acattgattg aatggtggga tcatgagtcc atgacaaata aaaccaccct ttatcctgaa
     1141 tcgtttttga taaacgatta tttttatcta aattagcctc aagcaagttc gacatatcca
     1201 tgtcgcaggc catcaatgca tcgcaaaatt cacttgagct ggttcgtgtt tcataactaa
     1261 actgagactt atcattttta cagagtacac ttcttaaaac ttctgtttgg cccgaaattt
     1321 gagccaagta ccaatgcagc agaaacagcg tcgttaggcg ttgctgacca tccaagggaa
     1381 caaatcgata cgagccctcg tcatctaaaa gactgccata gacaaaatct aaatctctat
     1441 ggggttttcc ttgctcaaga taggcataca acgcatctaa aaaactctcc cgcacctcat
     1501 gctctgaagg tcgcccctga gcgtaatccc gctgaataat gggtatttcc accttaaatt
     1561 ttttttcact gaaaagctta aaaaaactta ggcgttcacc tgatcctgag agtgttctga
     1621 cattattcat aagcaccctt ctgatctggc aagtattctt gtaaaattcc ttttatcgcg
     1681 ttcaagtatt catcggcatc cgattctttc caatacatca cttcacccaa ttttttacta
     1741 tagaatttca taaagacatt cttggtgcaa ataggcacaa aaacaccatt catatcatta
     1801 tgaataatgc gtcttcgctt aatgggaaac atcgcatttt tatagctacg attggttgta
     1861 gcatctagca gggccaaatt gccaacgcca tctaactcga aaacttcatc ctctttaaaa
     1921 taggaagcaa ttttctcata aagactttta aatgaactat catcaatttt gtctgcattc
     1981 aacaacttac gtaagtcgat aataaactca ccaatatcat ctgaaacccc atcgatggca
     2041 acaactgtat gatccctggt atcggcaaaa tactctaata catctttcgc ccactcgcgg
     2101 cgtaacgcgc ctgttatttc cttatctgtc tgagatcgga tgtgctcaat atcccaattc
     2161 tcctttttgt atcggtcaaa gggaaagcgc acatcggctt ctttggtaga taaaagtgtt
     2221 tgaatattga aaagtaaaag caccttacgc aattctttat caccataact gagcttgtct
     2281 aactggcaat tgacttcttt acgtatttcc tttttcagat aggctttaaa gtcagtcttt
     2341 gttggatcat tcttctctga ctcggattta agatctcgta ctgagtgccc gcaatccacc
     2401 aaaaaaccaa tcaaatgata caaatcacga tcacgatacc actcatcaaa agtgagaaaa
     2461 taatctttca cctccttcca gagagaatca atatccactt gcccgttcgc ttctttactt
     2521 gccttgaaag ccgcattaaa ttcatgaaag gtat
//
LOCUS       DATBTE010000447        19852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_881639, whole genome shotgun sequence.
ACCESSION   DATBTE010000447 DATBTE010000000
VERSION     DATBTE010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 19852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19852
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881639"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VIG33_16980"
     CDS             <1..567
                     /locus_tag="VIG33_16980"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine esterase"
                     /protein_id="HEY8272590.1"
                     /translation="PLKDYIPTEKKLNWLFPEGVTEVPIGPGWTGRAWWPLAMSTLST
                     DFSSNKPESLDKVRNDLMNMVAELKVPWSKIFLGGFSQGAMMATDLYLHAPEPPAGLL
                     LLSGALINKEELKPLIENRQGEKFFQCHGKNDQVLMMKSARQLESFLNAGGMKGGCLE
                     FPGGHEIPPQMLQKAGDYLNAQIRAKAL"
     gene            complement(632..1789)
                     /locus_tag="VIG33_16985"
     CDS             complement(632..1789)
                     /locus_tag="VIG33_16985"
                     /inference="COORDINATES: protein motif:HMM:NF022447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEY8272591.1"
                     /translation="MISMNPINLSYFPKDYEQSRTRFLQETSQHEGKPKLHQWKVPCA
                     RDQNLFVDSAYLPALNNKKRLFILISGTHGLEGYAGSGIQQLFLQEFLNKIDRSDSGF
                     LIIHSLNPFGFKYHRRGTEAGVNLNRNCSIEASFFQIQNRKSMEFAKQFIPTQPVASE
                     ISQLLKVMKKNGDGVLFDDVSMDEFIKVVGLGQFHDARGLEYGGAAPEPQIEALIHLL
                     KELIPQYKDVVHLDLHTGLGERGRLHLLIDGQPEALHPDLFAEILDPGLDKEVYDFTD
                     INDPGFYKTRGATNNLIAELITPAQRACALTLEFGTLGHSLEAQFKALNSWMLEHQGL
                     FYGYANKELENKVRDLYLERFFPSDSKWKEAILRVSYEFFKRVFSRSGILN"
     gene            complement(1903..2262)
                     /locus_tag="VIG33_16990"
     CDS             complement(1903..2262)
                     /locus_tag="VIG33_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272592.1"
                     /translation="MFNVVNISEFKPQSYSISLRQIHRKLDRTNLGPSLQIDAERNFI
                     ARIRGEFLSISRKKKNIPIEIVARYLRSDVEFIERMEAGIEPIYDYIFLRLSAYYGTL
                     NDAFALTNKIEKVLNPP"
     gene            complement(2246..2695)
                     /locus_tag="VIG33_16995"
     CDS             complement(2246..2695)
                     /locus_tag="VIG33_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272593.1"
                     /translation="MEDKKIKSPLGDIPNPFSAPEGVFTNIEEAKFAIRGVLRAYFGN
                     NNNDDEDDYLFFKASVVRFEENPLVDLELDHRRRIIIFIVKSPTGADGESLKMHIRNQ
                     ISMQFAVLASDARGRTAMSIYRALDPRAQQAVINSEKQKEKKDVQRR"
     gene            2889..3260
                     /locus_tag="VIG33_17000"
     CDS             2889..3260
                     /locus_tag="VIG33_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272594.1"
                     /translation="MSLHKSYLPNIEEMRKAAKITGLSLSTVNQAAINGKGSAITHGL
                     LICYGLKINPERIHEHLPKFRKVFSGSEKYSDLDENLQKVLKVFTVDEVIVTLEVMLA
                     KDKIERRFGIKKKAGRPASKS"
     gene            complement(3269..3505)
                     /locus_tag="VIG33_17005"
     CDS             complement(3269..3505)
                     /locus_tag="VIG33_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272595.1"
                     /translation="MKRSKKKNKHLASEGQMLMGQSQSELFQLQIELSGLSALFKNMG
                     DCELTSDELHGIGLSLERIAERVSASLSGLSSLP"
     gene            complement(3492..4085)
                     /locus_tag="VIG33_17010"
     CDS             complement(3492..4085)
                     /locus_tag="VIG33_17010"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEY8272596.1"
                     /translation="MDFDSITVPVVKGISCAQIENEAADFLQLFAPECLHAPQPTPIL
                     EIFENKMNLLGFDVLIGKNVKGLGGFTDITNRYIELPNSTYKKLEKGDPQARFTVAHE
                     FGHVKLHSAFQGKGQNHPVTFARRSRIRAFEDPEWQANSFSAAILMPIQTMRMLHAQD
                     KMTPAYVMATYQVSWPAASRRILRLRELRWRKNNEAE"
     gene            complement(4085..4477)
                     /locus_tag="VIG33_17015"
     CDS             complement(4085..4477)
                     /locus_tag="VIG33_17015"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8272597.1"
                     /translation="MSQEDIIPVIAESFASFLRRNRIATRKTMGQLARELGITVVYYS
                     EVEAGRKPAFPDKKVDYEILASSLGTTENQIKKIADLDREKRQILKVFECDHSSADLA
                     VAFGRRLSKNDLTAKQLSKIHKILNERE"
     gene            complement(4734..5585)
                     /locus_tag="VIG33_17020"
     CDS             complement(4734..5585)
                     /locus_tag="VIG33_17020"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HEY8272598.1"
                     /translation="MMDQLLIDPKRFEALITAPENLHKRVVIDEVQKFPKLLDTVHKH
                     IQKYKRQFILTGSSSRRLKQQGTNLLAGRAWVYHLYPFTSHELATLFDLKRTLEIGSL
                     PEAILAESFLDAREYLHSYVGTYLQKEIQQEQWVKNLEPFRKFLSIAAQMNGKIINKS
                     NMARDIGIHDTTIANYFQILEDTLIGFLLPAYHTSLRKAQKQAPKFYFIDPGIKRALD
                     RTLSVELLPQTAAWGEAFEHWIILEIKKNADYHRLDWEFSYLSTKDDVEIDLIIDRPG
                     EKNCSSK"
     gene            complement(5779..7743)
                     /locus_tag="VIG33_17025"
     CDS             complement(5779..7743)
                     /locus_tag="VIG33_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEY8272599.1"
                     /translation="MKSRIILVFAGVIVLWSALILRAAYLQFLPNEKLQSLQNRQFQT
                     VITLQARRGAIVDRNGRDLAMSATAYSLYADPKILDKRKQLAKNLAKELGVSMESIYA
                     KIKDSSKRFVWIQRLMDEDKAGKIKSWNVRGLSFVEEWKRVYPNESLLAHTLGFLGSE
                     GQGLEGLEKSFEADLKGNAKKVSVRRDARGRPLIADGLFFTENPDGNELKLTVDSDIQ
                     YNLENELAQAVTEFEADSAVGVILDAQTSAIIAMASVPTFDVNKAMKTSPELRRDKVA
                     TDAYEPGSTLKTFVIASALRQNLIAPNTKFFCEYGSFKVGDKVIHEAEASESFGNMTV
                     SEILAASSNIGTTKIAFKMGAESLRQGLVDFGFGNKLGLDLPGEARGSVQALPWRPHL
                     LSNISFGHGIAVTPLQMANAYAAIANGGVLKTPYIVQSLRDSETGEVKEIQPKPIRRV
                     LTPEQASQMKIMLSGTTAPGGTGKNARVNGFIVAGKTGTAQKVNPEGGGYIKGAYISS
                     FAGFIPANDPKFVIYVALDRPRKAFYGAQVAAPIFSRVASYAVRKEGLAPVHLSENTQ
                     ISEKRKSIKLDAEKSRGLTGIVEGDIVSTSLDLGLVPDVTSMTLREVLRRFNGKDMSV
                     KFRGQGVVTGVEPPVGSPLPENKEITVILR"
     gene            complement(7746..8084)
                     /locus_tag="VIG33_17030"
     CDS             complement(7746..8084)
                     /locus_tag="VIG33_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY8272600.1"
                     /translation="MNSENFRQLKPFCSVLILILTFFSLVFLQMEERRLGYSVLKLSR
                     EHKKILEEKRVREIQLVKVTRPQLVEHMAHSRFTLKKIQADQIIHLSGAAAGFVEMPR
                     AARADVKKEL"
     gene            complement(8065..8994)
                     /gene="rsmH"
                     /locus_tag="VIG33_17035"
     CDS             complement(8065..8994)
                     /gene="rsmH"
                     /locus_tag="VIG33_17035"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY8272601.1"
                     /translation="MKNILTKVEFTPEHYPVMVLDILENFAVFKDQKDLRYFDGTFGR
                     GGHLRAMLAAFPQVQAVVMDQDDTAVKFGLENFKTEVESQKVRIIHGNFSEFQEHNLG
                     SFDMMLLDLGVSSPQLDTADRGFSFNKEGPLDMRMNQTQGITAEYILNTASEQELNDM
                     FKEYGEIHRPFRVVRAIVHDRKTKAWQSTKELAGLIERVDGWRHKGFHPATQYFMALR
                     LRVNSELQVLEHALPAMIKSLKPGGRLAVISFHSLEDRIVKNIFKGNKDLGQQIHKRV
                     LLPTQEECDMNSRSRSAKLRVFERRVQDELREL"
     gene            9130..10053
                     /locus_tag="VIG33_17040"
     CDS             9130..10053
                     /locus_tag="VIG33_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272602.1"
                     /translation="METFIPPRKSAFVRYTTNPLLYLTLALIALSISIGTLYKKDHSD
                     LAQRVKFLKGTLQIANTYKQESKQPDEDTAESPTDPAEEMAAPAQVVPAAASTAVPTP
                     KAPPAPSGPVAFIYYAEVPRQSLEKIYEESLATGQFNSFGDYTAGILPDMQKRITAAG
                     LKIRILEKNEKPITKNQQWFTGIHDPEADEDLGLSTFVELTEIENNTFRGNLEIVRSL
                     RELDNFERTRNLASVDPAAIGNLQKTSYPAIFELSPGYGFFISGILPRKTHFTHEEEL
                     TAKAPFEILKSTNFQNKESEFVIFIEFEKKP"
     gene            10066..10848
                     /locus_tag="VIG33_17045"
     CDS             10066..10848
                     /locus_tag="VIG33_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEY8272603.1"
                     /translation="MQKEVQHHYGSQVHLINNPFLSGLLAKLCSPETFQPEINRLVEL
                     IYTQMISTVVNEEFDLESFKAPTRMTAYHANIDLQGTRVANTQQAVCVNLARAGTYPS
                     HICYNFLHFALPAQNIRQDHIFAARMADAENHVTGTQIGGLKIGGGVANAHVLIPDPM
                     GATGSTIVSTIDYYKKNIAGPAKKYIAIHLIVTPEYLKKVLTSHPEVVIYALRLDRGL
                     SNEAVLKSPAGLYWDQEKGLNDRDYIVPGGGGFGEIMNNSFV"
     gene            10880..11407
                     /gene="hpt"
                     /locus_tag="VIG33_17050"
     CDS             10880..11407
                     /gene="hpt"
                     /locus_tag="VIG33_17050"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEY8272604.1"
                     /translation="MTHLQIKPYISEEQLQKKVAEIGAAITKKYKGEQIVAVCVLKGS
                     FIFYSDLIREIDTDIHCEFFGVSSYHGSTSSGEVKVTLDLAHPVEGKHVLLVEDIVDT
                     GITMNYLKNSILARKPASLTTVALLEKPEALKVSCKLDYVGFQVANEFVVGYGLDYEG
                     YYRNLPYIGQVQNFQ"
     gene            complement(11392..12165)
                     /locus_tag="VIG33_17055"
     CDS             complement(11392..12165)
                     /locus_tag="VIG33_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272605.1"
                     /translation="MILEWLEYLKTQGNQTAKDWGYLYQNVSLKFRSRRCAKSWQSHI
                     NAAHQLIREHLEKLQPKTVMIIGSGLLLEIPIQDLMNKVEKIYLVDLVHAKEIRKLAA
                     KNKKIELIERDISSLLDVLKQGLGSFQLKTLPWNKLPAWDLPPVDWVISANLMSQIPL
                     MISERLPMSPEAYVKFAKSVRDQHIERLLEQAPQVLLFADFETHYIDREGQRIKTEDY
                     QVNLKSLKYDREWLWEISPFGETSRDYKIEMLVKAYWKF"
     gene            complement(12162..13757)
                     /locus_tag="VIG33_17060"
     CDS             complement(12162..13757)
                     /locus_tag="VIG33_17060"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HEY8272606.1"
                     /translation="MTAPLQKDILLVTLNSTYQHCAFGLRYLYANLHDLQPRAHILEW
                     TIHASPRNVVEKILSYNPRIVGFGVYIWNTTETFQIVSILKKVAPHVLIVLGGPEVTY
                     ESEDQAICKAADYVIKGEADHLFYEFCSKVLVQNAYSVEYPEQKFISGPAPDITQIQS
                     PYAYYTDEDIKNRIIYVEVSRGCPYKCEYCLSSLDKAVRSFNLDQFLADIEQLIQRGT
                     RQFKFVDRTFNLSIQTSSRILEFFLERIHLNLFLHFEMVPDRLPKELKELIKKFPHGT
                     LQFEVGIQTWNAEVARNVSRRNDFSKVHENFQFLAHESGVHTHADLIVGLPGESVQSF
                     GLGFDNLAACGPHEIQVGILKRLKGAPIARHDREFQMIYQEHPPFQILCNKDISYLQM
                     QQMNRFAKYWDLIANSGNFTNTISWIKQQAQFRQEKSFFWEFFALSEFLSQRFSETHS
                     LALQSLLEALWTYLTEVKKQAKPMVRDLLLSDYMKDKPRNIPNFLREGLNETAYQIHH
                     KIQSASNSLPDRQQRHLQNNNKA"
     gene            complement(13815..14222)
                     /locus_tag="VIG33_17065"
     CDS             complement(13815..14222)
                     /locus_tag="VIG33_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272607.1"
                     /translation="MKLNKSHLIVFLLAGSLVACVTEVKEPQDNKPYVATLAPEKGES
                     AAGTIVIKCRSKYDKDAKPTVCGASTVKLTNEITKSVSEHSFKGEKANLAVAGGGSFS
                     VEVKTKGCETKRVFSGMTGGMILVVQFDNCAVK"
     gene            complement(14212..15309)
                     /locus_tag="VIG33_17070"
     CDS             complement(14212..15309)
                     /locus_tag="VIG33_17070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY8272608.1"
                     /translation="MPANILIQTAFLGDLLLSIPLMKKMKELWPDQLLILVCRKGVGD
                     FFAKTHMVDEVLEVKKGDSESYRHVLERLKELKIDKLISPHESMRTSFFVQKIKANKK
                     ISFTKPWNRFFYNERIVKNYALPDPIRQLSLLQGMDVSLKAQIEKYSEEAKPYAVAKS
                     GRLAAPPEWSSMSLVDYYKSQGSEISKVLVKVHLNPTLLKKSIALFPGSVWATKRWTE
                     EGFVQVGQILSAQGTPVLIMGGPGEEELCARIAEKIPGASNLCSKTSIYESALLLSQV
                     AAVIGNDSASMHLAATSETPSVVIFGPTILKFGFRPWQKHIYVVEKSEMKCRPCGKHG
                     HNKCPIKTHACMKQISQDEVLEKLQGVLHET"
     gene            complement(15258..16412)
                     /locus_tag="VIG33_17075"
     CDS             complement(15258..16412)
                     /locus_tag="VIG33_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEY8272609.1"
                     /translation="MALVSACRHFSGYKPCGKNTSCEDSCPQKSIPSTSLLIIHLGAI
                     GAVLRTTSILKAMKRKFPGSQLTWVTDAPSDQLLRNHPLIDRVLTTKSEDLLSLSALE
                     FDAAFMIDKSLKAAGVLKQTYAEQVFGFRADARSGAIHPATPAAQELWCLGLDDHEKF
                     FLNQKAETQLLIEALELGPFQRDEYNLSLTFSEEREAHRRHNVWRQSHSLVLGINTGC
                     SSVIAYKKLSVEFQRQIIQTIKSELDVSVVLLGGPEDSERNLQIGEGLPIIQSSTTSG
                     LRDGLVSVAACDLILTGDSLGMHMAISQKKYVIAWFGPTCAQEIDLYDRGEKILSQAS
                     CSPCWKRLCDKPTMCYDQVSLMEIIEALKRGFSRCQQTSLSKQPSWETCS"
     gene            complement(16460..17590)
                     /locus_tag="VIG33_17080"
     CDS             complement(16460..17590)
                     /locus_tag="VIG33_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY8272610.1"
                     /translation="MKYFIYLIGCFLLLACSSSPLKYEKTKELEVNTEFDKAVKIEEV
                     PATDEGTAVSPTTPKKGNSKRNSKQKSKQNSARAAEPKATPSETEKILTPQKKDEAPK
                     NAGKSKKSKKNDQKVEESVPPLRRQPEFEDDTGFQGRRPLKDPFRVGEVVTHNVSYFK
                     TSAGSLRLKVEPMVNVNGRKAYNFVTEIVTSSMYSHIYSVDDRVETFVDFETLVPSVY
                     SLHVKESGQLREARMLFDDQTNRATFWEKKVTEKSGVEEKKQSWDILPYSQNVFSAIF
                     YMRNFQWEPGKEIAFRVADDEQNLIFKGKCLRRERIDTDIGERDAVVIKPEVTLKGAF
                     KPVGDIYIWLSDDERHYPLRIESKIKIGTLVSEVVSIIPGHN"
     gene            complement(17587..18471)
                     /locus_tag="VIG33_17085"
     CDS             complement(17587..18471)
                     /locus_tag="VIG33_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY8272611.1"
                     /translation="MKSLIKFCARIFSYFLFVLPRSWVRKLGGVVGFLWVDFFRVRRK
                     VIFGNLQIAFPEWSAEKRTKVGRESVYNMGYSFFEFFTVPYLDQTWLERHVVFEGEEH
                     LRKAREQGKGILALSLHLGNGDLASSAIVMKGQPITIITKVFKNSFFNDLWFSFRGAQ
                     GVKYIDAHGDKTSFDILKALKRQEAVVFVLDQFMGKPYGIETKFFGKKTGTAYGLSLF
                     AMRTRAPVVPIYTYEGADHKMHVVFEPEVPVLDLIGQDKDASILAITQRFNDKIEEVV
                     RKHPEHWMWVHRRWKDFE"
     gene            complement(18443..19456)
                     /gene="lpxK"
                     /locus_tag="VIG33_17090"
     CDS             complement(18443..19456)
                     /gene="lpxK"
                     /locus_tag="VIG33_17090"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEY8272612.1"
                     /translation="MSYLKPFSSLYEKIAGFKNYLYNQEYFQSVELPIPVLSLGNLTV
                     GGTGKTPLTDFCLKHYRRCKVKTAVVSRNYRAQVSGMTEVDLGHSDAAAYFGDEPVLL
                     AARNPEVHFFVGPKKFKTALYAFEKVKPALIIIDDGFQHRQLDRDVDIVILDATEPLE
                     NYQCLPEGRAREPWSSLSRASLFVVSKVNLVDSAKLEKLYKELRKFNKLIVPMSYELL
                     ALHGFKGEIPLAECQGQRVMLISGIAQPKAFEKSLEAFSLQVDCHFVFRDHHPYSAAD
                     VASIVEKWREKGSPRLMTTEKDFVKLKALWPVEVPLWYAPLEVKIQSQENGFYEILDQ
                     VLR"
     gene            complement(19453..>19852)
                     /locus_tag="VIG33_17095"
     CDS             complement(19453..>19852)
                     /locus_tag="VIG33_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEY8272613.1"
                     /translation="YIIMKEDPARMVHLADFILVASGTATLMVGLLQKPMVIMYRMKF
                     MTGVFARFLVRGVKFFGLVNLILGREVCPERWQSDANPETLFELMDRYFQDPQYAAKV
                     RSELGELRQHLGNKGATKRVAQSLEEFFTS"
BASE COUNT         5535 a         4701 c         4097 g         5519 t
ORIGIN      
        1 cccttgaagg attacattcc taccgaaaaa aaattaaatt ggctttttcc tgaaggtgtg
       61 acggaagttc ccattggacc agggtggact ggaagagctt ggtggcctct cgccatgagc
      121 accctttcca cggattttag ctcaaacaaa cccgaaagcc tcgacaaagt acgcaacgac
      181 ttaatgaaca tggtcgcgga gctcaaagtt ccttggagta aaatttttct tggcggtttt
      241 tcccaaggcg ccatgatggc caccgatctt tatttgcatg cacctgaacc acccgccggt
      301 ctgctgcttt tatccggcgc tctgattaac aaagaagaac tcaaaccctt aattgaaaat
      361 cgccaaggcg aaaaattctt ccaatgccac ggaaaaaatg atcaagtttt aatgatgaaa
      421 agcgcccgcc agctagagtc ctttctgaat gccggcggca tgaagggcgg ctgcctggaa
      481 ttccccgggg gccacgagat ccctccgcaa atgctgcaaa aagccggaga ctatttgaac
      541 gcgcagattc gggcaaaggc tttgtagtgt ttttcgagtt caatggcatt cgacataaca
      601 gatagaacac ttgatgaaat aaacagtgct tttaattcaa aatacccgag cggctaaaga
      661 cgcgcttgaa gaactcataa gagactcgaa gaatagcttc tttccatttt gaatcagacg
      721 gaaaaaatct ttctagataa agatctcgaa ctttattttc aagctctttg ttggcataac
      781 catagaagag tccctggtgc tcgagcatcc aactgtttaa ggctttaaac tgagcttcca
      841 ggctatgtcc gagagttcca aattccagag tcaatgcaca cgctcgttga gccggagtga
      901 tcaattctgc gataagattg tttgtcgctc cccgtgtttt ataaaaacct ggatcattaa
      961 tatctgtgaa atcgtaaact tctttatcaa gccccggatc gagaatttca gcaaaaagat
     1021 cagggtgaag cgcttcgggt tgcccatcga ttaataagtg caagcgtccg cgttcaccta
     1081 aacccgtgtg taaatccaaa tgcacgacgt ctttgtattg tggaattaat tcttttaata
     1141 aatgaattaa agcctctatc tgaggctcgg gagctgcgcc accgtattcc aatcctctgg
     1201 cgtcgtggaa ctgtccaagt cctaccacct taatgaattc atccatgctc acgtcatcga
     1261 acagaactcc gtcgccattt ttcttcatta cttttaacaa ctgcgaaatt tctgaagcca
     1321 ctggttgagt gggtatgaac tgctttgcaa actccattga ttttctattt tgaatttgaa
     1381 aaaaggaggc ttcgattgag caatttcgat tcagattcac tcctgcttca gttcctcgac
     1441 gatgatattt aaagccgaaa ggatttaaag aatgaatgat taaaaaacct gaatcagatc
     1501 tatcaatttt atttaaaaat tcttgcaaga aaagctgttg aatgccactg ccggcatagc
     1561 cttccaagcc atgggttccg gaaattaaaa taaaaagtct ttttttgtta ttaagagctg
     1621 gaagataggc ggaatctaca aaaagatttt gatccctcgc gcagggaact ttccattgat
     1681 gcagctttgg ctttccttca tgctgactcg tttcttgtaa aaaacgtgtt cgcgactgtt
     1741 catagtcctt aggaaaatag gatagattaa tgggattcat tgagatcatt ttctttatcc
     1801 ctgccggtaa gctcttccaa gcgcatggtt aaatctaatt ttattttttc atcgatatct
     1861 ttatttaaag aagttaacat gccaagagga agggtcaagg cattaaggag gatttagcac
     1921 tttttcaatt ttattcgtca gcgcaaaggc atcgttaaga gtcccatagt aagcgctcag
     1981 tcttaaaaaa atatagtcat aaataggttc tattcccgct tccattctct ctataaactc
     2041 cacgtcgctt cttaaatatc tggctacgat ttcaatagga atattctttt tctttcttga
     2101 aatcgaaaga aactcccctc ggatccttgc aatgaaattc ctttcagcgt ctatctgtaa
     2161 agaaggccct aaatttgtgc gatccaattt gcggtgaatt tgacgcaaag aaatggagta
     2221 agattgcggc ttgaattcag aaatattaac gacgttgaac atcttttttc tccttttgtt
     2281 tttctgagtt aatgaccgct tgttgggctc ttggatccaa ggcgcgataa atagacatcg
     2341 cggttcttcc gcgggcgtcc gaggcaagaa ccgcaaactg catggatatt tgattgcgaa
     2401 tgtgcatttt taaggactcc ccatcagccc ccgtcggcga cttcacaata aaaataataa
     2461 tacgacgacg atggtccagc tcaagatcca ctaagggatt ttcttcaaaa cgcacgacac
     2521 tggctttgaa aaaaagatag tcatcttcat catcattatt attattgccg aagtacgccc
     2581 tcagaactcc acggatcgca aacttggcct cttcaatatt cgtaaacact ccctcgggag
     2641 cagaaaacgg gttgggaata tctccaagag gcgatttaat ttttttatct tccattgcct
     2701 acatcctgcc actgtttata aatcacgtta agtgtaatat tatacgtatc gtaagtcaat
     2761 gggagcttat tttagtcatt ccaacaggtt atacatttat gaatttttgt gttaaattat
     2821 acgaaacgta tagaaagggt ccccctttgg aaaaatccgc ctctagcacc tccattagaa
     2881 attttctagt gagcctacac aaatcttatc tgccaaatat tgaagaaatg cgaaaggccg
     2941 cgaaaataac cggactctcc ttgtcaacag tgaatcaagc ggccatcaat ggaaaaggca
     3001 gtgcgatcac ccatggactt ttgatttgtt atggcctgaa aattaatccc gaaagaatcc
     3061 acgagcacct gcctaaattt cgcaaagtat tttccggctc tgaaaaatac tctgatttgg
     3121 acgaaaacct gcaaaaagtt ttaaaagttt ttacagtaga tgaagtcatt gtcaccctcg
     3181 aagtcatgct ggcaaaagat aaaatcgaac gtcgcttcgg tattaaaaaa aaggccggac
     3241 gcccagcctc taaatcctaa gacgggcttt atggcaggct ggaaagtcct gaaaggctcg
     3301 ctgaaacccg ttctgcaatt ctttccaggg aaagtccaat cccgtggagc tcatccgagg
     3361 tcagttcgca gtcacccata tttttaaaaa gtgcgcttaa tcccgaaagt tcaatttgca
     3421 attgaaaaag ctcgctctga gattgaccca taagcatttg accctctgaa gctaaatgct
     3481 tatttttttt cttactccgc ttcattattt tttctccatc gaagctctct gagccttaag
     3541 attcgtcgac tggccgcagg ccatgagacc tgataagttg ccataacata agcaggagtc
     3601 atcttatcct gggcgtgaag catacgcata gtttgaatag gcatcaagat agctgcggaa
     3661 aaactattag cctgccactc aggatcctca aaagcacgaa tgcgagagcg acgggcaaag
     3721 gtcaccggat ggttttgtcc ttttccctga aaagcactgt gaagcttcac atgaccaaac
     3781 tcgtgggcca ctgtaaatcg agcttgagga tcgccttttt ccaatttttt ataagtggaa
     3841 tttggcaact caatgtatct atttgtgata tccgtaaatc cgccaagacc tttgacattt
     3901 tttccaataa ggacatcaaa acccaagaga ttcattttat tttcaaagat ctctaaaata
     3961 ggagttggct ggggggcatg caagcactct ggagcgaaca attgcagaaa atccgcagct
     4021 tcattttcaa tttgagcaca ggaaatcccc tttacaacag ggactgtaat agaatcaaaa
     4081 tccatcactc cctctcgttg agaattttat gaattttaga caattgcttt gcggtaagat
     4141 cattttttga tagacgtcgg ccaaaagcca cagctaagtc cgcgctggaa tgatcacact
     4201 caaagacttt aagaatttgt cttttctcac gatccaggtc agcaattttt ttgatttgat
     4261 tttctgtggt gcccaaggag gaagctagaa tttcataatc gacttttttg tcgggaaacg
     4321 caggttttcg tccggcttcg acctcagaat agtagacaac cgtgatcccc agttccctcg
     4381 ccaactggcc catagtctta cgagttgcaa tacggtttct tcttaagaag ctggcaaagc
     4441 tttcagcaat gaccggaata atatcttctt ggctcataaa tactttttcc tctattagct
     4501 aacgttagct aatagatgtt ggtaaacttg taaatattta acgcaaaagg atatgaatat
     4561 ttatttgaag tcgaacaact cctgtaaagc tttctgccaa tgcaaagctc tggttgatcc
     4621 gaatttttgt tcaaggggat cacacgagag aagccatctt tcactcttag gtatgtcttt
     4681 acctaaattt tcaagagctt tggcgtccct ttctccgact ttatcttttg attttatttc
     4741 gatgagcaat ttttttcacc aggtcggtca atgatcagat caatctccac atcatccttc
     4801 gtgctaagat aagagaactc ccagtctaaa cgatgatagt ctgcgttttt tttaatttct
     4861 aagatgatcc aatgctcaaa agcctcaccc caagccgccg tttgtggaag aagctctacc
     4921 gagagagttc tatccaaagc tcgtttaatt cctggatcga taaagtaaaa ctttggtgct
     4981 tgcttttgtg cttttcgcaa agaggtatgg taagcaggca aaagaaagcc aatcaaggtg
     5041 tcttcaagaa tctgaaaata attggcgatg gttgtatcgt gaatgccgat atcccgagcc
     5101 atatttgact tattgatgat cttaccgttc atttgggcgg cgatagaaag aaactttcta
     5161 aaaggctcaa gatttttcac ccattgttct tgttgaattt ctttttgcaa atacgttcca
     5221 acataggaat gaaggtattc tcgagcatca aggaatgact ccgctagtat tgcctcagga
     5281 aggcttccga tctctaaagt tctttttaaa tcaaagagag ttgcaagttc gtggctggta
     5341 aaaggataaa gatgatagac ccaagctctg ccagcaagaa gatttgtacc ctgctgtttc
     5401 aatcttcggc tgctcgatcc tgtaagaata aattggcgct tgtacttttg aatatgttta
     5461 tggacggtgt ctaataattt tgggaacttt tgaacttcat caatgacgac tcttttatgc
     5521 aaattttcag gcgccgtaat aagagcctca aaacgtttag gatcaattaa cagttgatcc
     5581 atcatatcca aatctaagag atcaacaaag cgtttcttta tcgggaaaga gagcatgcaa
     5641 gagggtcgtt ttccctgttc ccctcggacc aaacaagaaa aaactgtttg tttttgataa
     5701 cttacaatat ctctggatca caatcagaat tttaacgctt ttttctggat gatgtcaaga
     5761 gcgtgaaaac ttcttcaatc aacgcagaat cacagtgatt tccttattct ccggaagagg
     5821 cgagcccacc ggaggctcga ctcccgtgac gacaccctgg ccgcgaaatt tcacactcat
     5881 gtctttgcca ttgaatcgtc tgagcacttc ccgcaaagtc atacttgtca catcagggac
     5941 tagacccaag tccaaactcg tcgaaacaat atctccttca acaattccag taagtcctcg
     6001 agatttttca gcatccagct tgattgattt tcttttttct gaaatctgcg tattctctga
     6061 caaatgcacc ggagccaatc cctctttacg aacggcgtag gaagccactc ttgaaaaaat
     6121 cggtgccgct acctgagctc catagaaagc tttgcgaggg cgatcaagag cgacgtagat
     6181 cacaaacttc ggatcattgg ccggaataaa tccggcaaaa ctcgaaatat aggcgccctt
     6241 aatgtagccg ccgccctcag ggtttacttt ttgcgctgtc cctgtttttc cggcaacaat
     6301 gaagccgttg acgcgagcgt ttttgccagt tccacccgga gccgttgtac cgctcaacat
     6361 gattttcatt tgcgacgctt gctcgggagt taaaactcgg cgaatcggtt taggctggat
     6421 ctctttgacc tcaccggttt cactgtcgcg cagggattgc acaatgtaag gagtcttcaa
     6481 gactccgccg ttggcgatag cggcataggc attcgccatt tgcaaagggg tcaccgcaat
     6541 gccgtgaccg aaagaaatat tactgagaag atgcgggcgc caagggagag cttgaacgct
     6601 gccacgggct tccccaggaa ggtccagacc cagtttattg ccaaaaccaa aatccacgag
     6661 gccttggcgg aggctctctg ctcccatttt gaaagcgatc tttgtggttc cgatatttga
     6721 agaggccgca aggatttcac tcaccgtcat attgccaaag ctctcgctgg cttccgcttc
     6781 gtgaataact ttgtcgccca ctttaaagga accgtactca caaaagaatt tggtattagg
     6841 ggctatgaga ttttgccgaa gtgctgaggc aatcacgaaa gtttttaaag tgctccccgg
     6901 ctcataggca tcggtcgcca ctttatcgcg gcgaagctcc ggtgaagttt tcatcgcttt
     6961 attcacatcg aatgtcggta cgctggccat ggcaatgatc gccgaggtct gtgcatccaa
     7021 aatcactccc accgccgagt ccgcttcgaa ttcagtcacc gcttgagcca attcattttc
     7081 aagattgtac tgaatatcag aatccaccgt gagcttcagt tcattgccat ctggattttc
     7141 cgtaaaaaac aagccatccg caatcaaagg acgaccgcgg gcatcgcgac ggacggacac
     7201 tttcttggca ttgcccttga gatcggcttc aaaagatttt tcgagccctt ccaagccctg
     7261 cccttcactg cccaagaacc ccaaagtatg ggctaataaa gattcattcg gataaactct
     7321 tttccactcc tctacaaagg aaagaccccg gacattccaa gatttaattt tgccggcttt
     7381 gtcctcgtcc ataagacgtt gaatccaaac aaagcgtttg ctggaatctt taatctttgc
     7441 atagatactt tccatgctca cgcccaactc tttggcgaga ttctttgcga gttgcttgcg
     7501 tttatccaag attttggggt cggcatataa agaataggcc gtcgccgaca tggccaggtc
     7561 ccggccattg cgatccacaa tcgctccgcg acgagcctgc aaagtgatca ccgtttgaaa
     7621 ttgacggttt tgtaaagatt gaagcttttc attcggcaaa aattgcagat aggccgctcg
     7681 caagatcaag gcactccaaa gaacgatcac acctgcaaat actaagatga ttcgtgattt
     7741 caattctata gctccttttt aacatctgcg cgagccgccc gaggcatctc cacaaaaccg
     7801 gcagcggctc cgctcaaatg gatgatttga tccgcttgaa ttttcttaag agtgaaccgg
     7861 gagtgagcca tatgctccac caactgggga cgcgtcacct tcacaagctg aatttcgcgg
     7921 acccgctttt cttcaagaat ctttttgtgt tcacggctga gcttcaaaac gctataaccc
     7981 aaacgacgtt cttccatttg taagaagacc agggaaaaga aagtaagaat aagaattaaa
     8041 acactgcaaa aaggtttaag ctgtctaaag ttctctgagt tcatcctgaa ctctcctctc
     8101 aaaaactcga agcttcgcac tgcgcgagcg ggagttcatg tcacactctt cctgagtggg
     8161 taataagact ctcttgtgga tttgttgacc caagtctttg ttgcctttaa aaatattttt
     8221 aacaatacga tcttccaagg aatggaagct gatcaccgcc aagcgtccgc ccggtttcaa
     8281 ggatttgatc attgctggca aagcatgttc cagaacctgt aactctgaat tcacccgtaa
     8341 tcgaagcgcc ataaaatatt gagtggcggg atggaagcct ttatggcgcc agccatcgac
     8401 tcgctcaatg aggccagcaa gttctttggt gctttgccac gccttggttt tgcgatcatg
     8461 gacgatcgcg cgaaccaccc gaaaaggacg gtgaatttct ccgtactctt taaacatatc
     8521 gttgagctcc tgctcagacg ccgtattcaa aatatactcc gccgtaatcc cttgggtttg
     8581 gttcatacgc atatcaagag ggccctcttt attaaaacta aacccacgat ctgccgtatc
     8641 aagctgaggc gaactcaccc ccagatctaa caacatcata tcaaaagaac ctaaattgtg
     8701 ctcttgaaac tctgaaaaat ttccatgaat tatgcgcacc ttctgggact cgacttcagt
     8761 cttaaaattt tccagaccga actttacggc ggtgtcatct tggtccatga ccacagcttg
     8821 gacctgggga aaagccgcga gcatggcccg cagatgtcct cctcggccaa aggttccatc
     8881 gaaatagcga aggtcttttt ggtctttgaa aacagcgaaa ttttcaagaa tatcaagcac
     8941 catcaccgga tagtgctctg gcgtgaactc aactttagtt aggatatttt tcatgtgatt
     9001 ctcaaggtat gaacaatttg tttgtgggtc aagaccctat gatttaagct ataaggaatg
     9061 ctggtcaaat gtccacgttg tgggttttca caacctcaag ataaatactg cgcccaatgc
     9121 ggcatcgata tggagacttt catacctcct cgtaaatccg cattcgttcg ctatacaacc
     9181 aatcccttac tctatttgac attggctttg attgccctga gcatttccat tggcaccctc
     9241 tacaaaaaag accattccga cctggcgcag agagtaaaat ttcttaaggg caccttacaa
     9301 atcgctaata cttataaaca agagagtaag caacctgatg aggacactgc ggagagtcct
     9361 acggatccgg cagaagaaat ggcagcgcca gcacaggtag ttcccgctgc cgccagcact
     9421 gcagtaccaa cgccaaaggc acctccagcg ccttcggggc cagtcgcctt tatttattat
     9481 gctgaagttc ctcgtcaaag ccttgaaaaa atctatgagg agagcctcgc aacgggacaa
     9541 tttaactctt tcggcgatta tacggctggc attctgcctg atatgcaaaa acgcattact
     9601 gcggcagggc ttaaaatccg cattcttgaa aagaatgaaa aacctattac taaaaatcag
     9661 caatggttta caggaatcca tgatcctgaa gccgatgagg atttaggctt gagcaccttt
     9721 gttgaactga ccgaaatcga aaataacacc tttcgcggaa atcttgagat cgtgcgcagc
     9781 ctgcgtgagc tggataattt tgagcgaacc cgtaacctcg cgtcggtgga tcctgcagcc
     9841 attggaaacc tgcaaaagac ttcttacccc gctattttcg aactgagccc cggctatggc
     9901 tttttcatct ctggcatcct gccgcgtaag acacatttta cccatgagga agaacttaca
     9961 gcaaaagcac cctttgagat tttaaaatcc accaatttcc agaacaaaga gtctgaattc
    10021 gtcattttca ttgaatttga gaaaaaacct taagatatta tctccatgca aaaagaggtt
    10081 cagcaccact acggctctca agttcatttg atcaataatc cttttttgag cggtctgctc
    10141 gcaaagcttt gctctcctga aactttccag ccagaaatca atcgcttggt tgaattgatt
    10201 tacacgcaaa tgatctcaac cgtggtgaat gaagaatttg atctggagtc gtttaaggca
    10261 cccacgcgta tgacggctta ccatgccaac attgatttgc agggcactcg cgtcgccaac
    10321 acacaacagg ctgtttgtgt aaatctggcc agagcgggaa cttatccaag ccatatttgt
    10381 tataattttt tacatttcgc tctaccggcg caaaacatcc gtcaagatca catcttcgcc
    10441 gcccgcatgg ctgatgccga aaatcatgtc accggcaccc aaatcggtgg acttaaaatt
    10501 gggggcggcg tcgccaatgc ccacgtactt ataccagatc ctatgggagc cacaggcagc
    10561 acgattgtct ccaccattga ttattacaaa aaaaacattg cgggtccggc gaaaaagtac
    10621 atcgccattc acctcattgt cacaccagag tatttgaaga aggttttaac ctctcatcca
    10681 gaagtcgtga tctatgcctt gagacttgac cgcggccttt ccaatgaggc agttttaaaa
    10741 tctccggcag ggctttattg ggatcaagaa aaaggtctca atgatcgaga ctacatcgtt
    10801 cctggtggcg gcggctttgg tgaaataatg aataactcct ttgtataaaa agaaaatatc
    10861 ataagcaaaa ggaagcaaaa tgacccatct tcagatcaag ccttacatct cagaagaaca
    10921 actccaaaaa aaagtcgctg aaatcggcgc ggccattacc aaaaagtata agggcgaaca
    10981 aatcgtcgcg gtttgtgtgt taaaaggttc ttttattttt tactcggatc tcatccgtga
    11041 aattgatact gatatccatt gtgaattctt cggcgtctcc agttatcatg gctccacttc
    11101 ttctggggaa gtgaaggtca ccttggatct ggcccacccg gttgaaggca aacatgtttt
    11161 gctagtggaa gacattgtcg atacgggcat cacgatgaat tacttaaaaa attccatcct
    11221 cgccagaaag ccggcctctt taaccaccgt ggccctgcta gaaaaaccag aagctttaaa
    11281 agtttcttgc aagctggatt atgtggggtt ccaagtcgcc aatgaatttg ttgtgggtta
    11341 tggtctggat tatgaaggtt actatcgcaa ccttccttac atcggccaag ttcagaattt
    11401 ccaataagct ttcacgagca tttcaatttt atagtcgcgg gaggtctcac caaagggtga
    11461 gatttcccaa agccattccc ggtcatattt cagagatttc aaattcactt gatagtcttc
    11521 agttttaatt ctttggccct ctcgatcaat atagtgagtt tcaaaatcag caaagagaag
    11581 cacttgagga gcttgctcaa gaagtctctc gatatgttga tcacgcacgg atttcgcaaa
    11641 tttcacgtaa gcctcagggc tcattggcaa tctctctgaa atcatcagag gaatctgcga
    11701 cattaaattg gccgaaatca cccaatcgac tgggggcaaa tcccaggcag gcaacttatt
    11761 ccaaggaagg gtctttaatt gaaaagaacc aagtccttgt ttaagaacat ctagcaggct
    11821 tgagatgtct ctctcgatca actcgatttt tttattctta gccgccaatt tgcgaatttc
    11881 cttggcgtga accagatcca ccagatagat tttttcaacc ttattcatga gatcctgaat
    11941 aggaatctct agcaacaaac cgctgccgat gatcatgacc gttttgggct ggagcttctc
    12001 cagatgctcg cggattaatt ggtgagccgc attgatatgg ctctgccaag acttcgcgca
    12061 acggcgtgaa cgaaatttta aagagacgtt ttgataaagg tagccccagt cctttgctgt
    12121 ctgattgcct tgagttttaa gatactccaa ccactcgaga atcatgcttt gttattattc
    12181 tgcagatgac gttgttggcg gtccggcaac gaattcgaag cactctggat tttatgatgg
    12241 atctggtaag ccgtctcatt gaggccctca cgcaagaaat tcggaatatt gcggggttta
    12301 tctttcatat agtcactcaa aagcagatcc ctgaccatgg gctttgcttg ctttttgacc
    12361 tcagtaagat aagtccaaag tgcttccaaa aggctctgca aagccaggct atgggtttcg
    12421 gaaaaacgct ggctcagaaa ttcactgagg gcgaaaaatt cccagaaaaa agatttttct
    12481 tgtcggaatt gggcctgttg tttgatccag gaaatcgtat tggtaaaatt cccgctgttg
    12541 gcgatcaagt cccaatattt tgcaaaacga ttcatctgtt gcatctgtag ataagaaatg
    12601 tccttgttgc aaagaatctg aaagggcgga tgctcctgat aaatcatctg gaactcgcga
    12661 tcatgacgag caatgggagc gcccttcaaa cgttttaaga ttccaacctg aatttcgtga
    12721 ggtccacagg ccgctagatt atcaaaaccg agcccaaagc tctggacact ttctcccggc
    12781 aggccaacga tcaaatcggc atgagtatga accccgcttt catgcgccaa gaactgaaaa
    12841 ttttcatgaa ccttggagaa atcgttgcga cgactgacat ttctagcaac ttcggcattc
    12901 caagtttgaa tacccacctc aaactgcaaa gtaccgtggg gaaatttctt gatcagctct
    12961 ttgagctcct tgggcaatcg atccggtacc atttcaaaat gcaaaaataa atttaagtga
    13021 attcgttcaa gaaaaaactc taaaatacga gagctggttt gaatactcag attaaaagtg
    13081 cgatccacaa acttaaactg tctggtgcct ctttgaatca gttgttctat gtcggccaag
    13141 aattgatcca gattaaagct gcggacagct ttatccaatg aagacaagca atactcacat
    13201 ttgtaagggc agccgcgaga gacttcaaca tagatgatac gatttttaat atcttcatcg
    13261 gtgtaatagg cataaggaga ttgaatctgg gtgatatctg gagcaggtcc tgagataaat
    13321 ttctgctctg gatattctac ggaataagca ttttgcacta gtacttttga gcaaaactca
    13381 taaaataaat gatcggcttc accctttata acataatctg cagctttgca aatcgcctga
    13441 tcttcacttt cgtaagtgac ctctgggccc cctagaacaa ttaatacatg gggagccact
    13501 ttctttaaaa tggatacaat ctgaaaagtc tcggtcgtat tccaaatata caccccaaaa
    13561 cccacaatcc ttggattata actcaagatc ttctctacga cattgcgagg gcttgcatgg
    13621 attgtccact cgagaatgtg agctcgaggc tgtaaatcgt gaagattcgc atagaggtaa
    13681 cgcagaccga aagcacagtg ctgataagtc gagttcagag tcactagaag aatgtctttt
    13741 tgtaggggcg ccgtcatatc cctgctttaa accaaagccc catgtttttg gcaagtcgtt
    13801 tgctcataga attattattt aacagcgcag ttatcaaatt gtacgacgag aatcatcccg
    13861 ccggtcattc cggaaaatac tcgctttgtc tcacagccct tggtttttac ttccaccgaa
    13921 aaggatcctc ccccggcaac ggcaaggttt gccttttcgc ctttaaagct gtgttcagaa
    13981 accgatttgg tgatttcgtt cgtaagtttc acagtgctag ctccgcaaac tgtgggctta
    14041 gcgtctttat catatttaga acgacacttg atcacaatag ttcctgcagc tgattctccc
    14101 ttttcagggg caagggtcgc cacatagggc ttgttatcct gtggctcttt cacttccgtc
    14161 acgcaagcca ccaatgaacc tgccaataaa aatacaatta agtgggattt attaagtttc
    14221 atgaagaact ccttgaagtt tctcaagcac ttcgtcttgt gaaatttgct tcatacacgc
    14281 atgggtttta atggggcatt tgttatggcc atgtttccca caaggtcggc atttcatctc
    14341 gcttttctca acaacataga tatgcttttg ccaaggtcta aatccaaatt ttaaaatcgt
    14401 ggggccaaag atgactactg agggcgtttc gctcgtcgcg gctaaatgca tcgaagcact
    14461 gtcattgcca ataaccgccg ccacctgcga caagagcaat gcggattcgt aaatgctggt
    14521 ttttgaacaa agatttgaag ctccaggaat cttttctgca attcttgcac aaagctcttc
    14581 ttctcccggt ccccccatta ttaaaactgg agtcccctgt gcggataaga tctgcccaac
    14641 ttgcacaaag ccctcttctg tccatcgctt ggtcgcccaa acacttcctg gaaacaaggc
    14701 tatcgacttt ttaagaagag tgggattcaa atgaactttt acaaggactt tggaaatttc
    14761 acttccctgg cttttataat aatcaaccag actcatggaa gaccattccg gaggagccgc
    14821 cagcctccca ctttttgcta cagcataggg ctttgcctct tcagaatatt tttcgatttg
    14881 agcctttaag gaaacatcca ttccctgcaa aaggctgagc tgacgaattg gatcgggcaa
    14941 agcgtaattc ttaacaattc gttcattgta aaaaaatcgg ttccaaggct tcgtaaaaga
    15001 aattttttta ttggctttga ttttttgcac aaaaaaagag gttcgcatcg attcatgggg
    15061 agaaatcaac ttgtctattt taagctcttt taatctctcc agcacatgac ggtaagattc
    15121 actatcccct tttttaacct caagaacctc gtccaccata tgagtttttg caaagaagtc
    15181 acccactccc ttacggcaaa ccaaaatcaa gagctggtca ggccacagtt ctttcatctt
    15241 tttcatcaac ggaatgctta agagcaagtc tcccaggaag gctgtttgga taaggatgtt
    15301 tgctggcacc gactaaaacc tctcttaaga gcttctataa tttccattaa agaaacctga
    15361 tcgtaacaca tcgtcggctt atcgcatagt ctcttccagc aaggagagca agaagcctga
    15421 gagagaattt tctcgccccg atcgtagagg tcaatttcct gcgcgcatgt cggtccaaac
    15481 caggcaatga cgtatttttt ttgagaaatg gccatgtgca tacccaggct gtctccagtt
    15541 aaaatgagat cacaggccgc gacgctgacc aaaccgtcgc gaagtcctga agtcgttgaa
    15601 ctttgaataa tgggaagacc ctccccaatt tgcagatttc gttcactgtc ttcgggtcct
    15661 cctaaaagca ccacactcac gtccaactca cttttaattg tttgaattat ttggcgctga
    15721 aattccacag acagtttttt atatgcaatc accgaactgc aaccagtgtt gatccctagc
    15781 accaagctat gactttgcct ccaaacattg tgccgacggt gcgcctctct ttcttccgag
    15841 aaggtcaggc tcaaattgta ttcatcccgt tgaaacgggc ctaattcaag ggcctcaatc
    15901 agcaattgag tctccgcttt ctgattcaag aaaaattttt catgatcatc aagcccgagg
    15961 caccacaact cctgagcggc cggagtagcc gggtgaatag ctccacttct cgcatccgct
    16021 cgaaatccaa aaacctgctc agcataagtt tgcttaagaa cccctgccgc ttttaaagac
    16081 ttatcaatca tgaaggccgc atcgaactcc aaagctgaaa ggcttaagag gtcttctgat
    16141 ttagtggtca acaccctatc aatcaaagga tgattccgca ggagttggtc actgggggca
    16201 tccgtgaccc aggtgagctg gctgccggga aatttgcgtt tcatcgcttt taaaatactg
    16261 gtggtcctga ggactgcacc gatagcgcct aagtggatga tcaacaaaga agtgctggga
    16321 atggacttct gcgggcaaga gtcctcacag gaagtgtttt taccacaggg tttatagcca
    16381 gaaaaatgtc ggcacgcact tactaaagcc atgggtcatt ttacttggct tttgcgctaa
    16441 agaggactcg accttgtgct taattatgtc caggaatgat cgagacaact tcagaaacca
    16501 aggttccgat cttaatttta gactcgatcc gcagcggata atgacgttca tcatctgaaa
    16561 gccagatata gatatctcct accggcttaa acgccccctt cagagtcacc tccggtttga
    16621 tcacgacagc gtctctttca ccaatgtcag tgtcaattct ttcacgacga agacattttc
    16681 ctttaaagat cagattttgt tcatcatctg ccacgcggaa ggcaatttct ttacccggtt
    16741 cccattgaaa attccgcata taaaaaatcg cactaaaaac attctgggaa taaggcaaaa
    16801 tatcccagct ttgttttttt tcttcgacgc cgcttttctc ggtcactttc ttttcccaaa
    16861 aagtggcgcg attggtctgg tcgtcaaaaa gcatgcgggc ttcacgcaat tgcccggatt
    16921 cttttacatg caatgagtaa acgcttggga ccaaagtctc aaaatccaca aaagtttcga
    16981 cccggtcatc aacgctatag atgtgagaat acatggagct agtaacaatt tcagtaacaa
    17041 agttgtaagc tttgcgacca ttcacattca ccattggttc cactttcaat ctcaaacttc
    17101 cggcggaggt tttaaaatag ctgacattgt gagtgaccac ttcgccaaca cggaaagggt
    17161 ctttaagtgg acgacgtcct tgaaatccag tatcgtcttc aaactccggc tgacgtctta
    17221 gaggcggcac ggactcttcg actttctggt cattcttttt tgacttcttg gattttccgg
    17281 cgtttttagg ggcttcatcc tttttctgtg gggttaatat tttctcggtt tcggaagggg
    17341 tcgccttagg ctcagccgct cttgctgagt tctgttttga cttctgcttt gagtttcgtt
    17401 ttgagttccc tttcttcggc gtcgtcggag aaactgcagt tccctcatca gtcgccggaa
    17461 cttcttcgat tttaacggct ttatcgaact cggtattgac ctcaagctct ttggtttttt
    17521 catacttcag cggcgatgag gagcatgcaa gaagtagaaa acagccgatt aaatatataa
    17581 aatatttcac tcgaaatcct tccaacgccg gtgcacccac atccagtgtt caggatgctt
    17641 tctcacaacc tcctcaatct tatcattgaa cctttgggtg atggcaagaa tgcttgcatc
    17701 tttgtcctgc cctataaggt ccaggacagg aacctccggt tcaaagacca cgtgcatttt
    17761 atgatcagcg ccttcatagg tgtagatggg cacaaccgga gccctggtcc tcatggcaaa
    17821 aagagacagt ccataggccg ttcccgtttt tttgccaaaa aacttggttt caattccata
    17881 gggcttcccc ataaattgat cgagcacaaa aaccacggct tcttgccgct tcagggcttt
    17941 taaaatatca aaactggtct tatctccatg agcatcaata tatttcacgc cctgagctcc
    18001 gcgaaacgaa aaccaaagat cgttaaaaaa cgaattctta aaaaccttgg tgataatcgt
    18061 gatcggctgg cctttcatca caatcgcact cgaggccaaa tctccatttc ctagatgcaa
    18121 actgagggct aggatccctt tcccttgctc ccgggccttt cgcagatgtt cttcgccttc
    18181 aaacaccaca tgtctttcaa gccaggtttg atcgagataa gggaccgtga aaaattcgaa
    18241 aaaactatat cccatattat agacgctctc gcgcccgact ttggtccgct tttccgccga
    18301 ccattcgggg aaagcaattt gcaaatttcc aaaaatgacc ttgcggcgga cacgaaagaa
    18361 atccacccat aaaaagccga caactccgcc cagctttctc acccaagaac gggggagcac
    18421 aaaaagaaag taactaaaga ttctagcgca gaacttgatc aaggatttca taaaacccat
    18481 tttcctgaga ttgaatctta acttctaaag gggcatacca caaaggcact tcaaccggcc
    18541 acaaagcttt caactttaca aaatctttct cggtcgtcat cagtcgcgga gatccttttt
    18601 cgcgccattt ttcaacaata gaagccacat ccgcggcgct gtaaggatga tgatctctaa
    18661 acacaaagtg acagtctact tgtaaagaaa aagcttcaag gcttttctca aatgcctttg
    18721 gctgtgcgat acccgagatc agcatcaccc tttgtccttg gcactctgcc aagggtattt
    18781 cacctttaaa accatgaaga gccaaaagtt catagctcat cggcacgatg agcttattga
    18841 atttacgcag ctctttatat aacttttcaa gctttgcaga atctaccaga ttcactttac
    18901 tcactacaaa taaggatgcc ctacttaaag aactccaagg ctcacgcgca cgtccttcag
    18961 gcaagcactg atagttttct aaaggttccg tggcatccag aatgacgatg tccacgtcgc
    19021 gatccaattg acgatgctga aacccatcat caatgataat caatgctggc tttactttct
    19081 cgaaagcata gagggcggtt ttaaattttt taggaccgac aaaaaaatga acctctggat
    19141 ttctcgcggc caaaagcacc ggttcatccc caaaataagc ggcagcatcg gagtgcccca
    19201 aatcgacctc agtcatccct gaaacttgag cacggtaatt acggctgacc acggcggttt
    19261 taaccttaca gcgtcggtag tgtttcaaac agaaatctgt aagcggggtt tttccggtcc
    19321 cgcccactgt caggtttcca aggcttaaaa ccggaatcgg aagctccact gattgaaaat
    19381 actcttggtt ataaagatag tttttaaaac cggcgatctt ttcgtacaaa gagctaaacg
    19441 gttttagata actcatgacg tgaaaaactc ctcaagagat tgagccaccc gtttcgtcgc
    19501 gcctttatta cctaaatgct ggcgaagttc gcccaactcc gagcgaactt ttgccgcata
    19561 ctgcggatct tgaaaataac ggtccatcaa ttcaaataaa gtttcaggat tggcatcaga
    19621 ctgccaacgc tctggacaga cttcgcgacc cagaatcaaa ttcaccaggc caaagaattt
    19681 caccccacgg accagaaagc gtgcaaacac tccggtcata aatttcatgc gatacatgat
    19741 aaccattggc ttttgcagca accccaccat tagggtggcg gtaccggaag ccaccaatat
    19801 aaaatccgca agatggacca tgcgcgcggg gtcttctttc ataatgatat aa
//
LOCUS       DATBTE010000448         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1496606, whole genome shotgun sequence.
ACCESSION   DATBTE010000448 DATBTE010000000
VERSION     DATBTE010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496606"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..966)
                     /locus_tag="VIG33_17100"
     CDS             complement(232..966)
                     /locus_tag="VIG33_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272614.1"
                     /translation="MTKIILVFATVFAINAQAADQNDVKYFQLAKVKVTEVTNQYPLT
                     ETFTQPGLGEDCNPASRAKVLDNGVPSLNPADPLDQISIIIDKIVNIGKKVWAIVETG
                     RPTVHIKTYTANALPQGLKCWSDLTGWQIPQSKVYRVQYENLYGMDVVDFAYRVTFTA
                     GGALKGQGKYITNATIMPADLNVSWGYTFNANAEVPSVFNMGTSEQPVAGMQMVMKWS
                     VDTVMNHAEQAESYYVGGDNTMKQME"
     gene            complement(1077..1637)
                     /locus_tag="VIG33_17105"
     CDS             complement(1077..1637)
                     /locus_tag="VIG33_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272615.1"
                     /translation="MRAALLFLICHLISSVSFAEAHLDFSAIYSMDKLNASSEAANTQ
                     YFYNVDVLFNIDRRMQWNVGWSVLGISQTSTLDGATATYSSFDIGPALRWNIDKAGIF
                     SMTLAYGYLAKGQYNSGNTLETWTGSSYFGQFAVQAPIADKLYVGFSLNYYAAGYSKK
                     VVDSVESSSTAQKNWIFPMISFTWRP"
     gene            complement(1690..>2926)
                     /locus_tag="VIG33_17110"
     CDS             complement(1690..>2926)
                     /locus_tag="VIG33_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY8272616.1"
                     /translation="TGPQDSSIKFHRHLVENIVNAVTEIFNQARYADKVIERVLKSEK
                     KWGGRDRRFVAESIYEMVRWWRYLGGLAGFEPTEKSMSREDVWQLWAAWCFNTYQILP
                     EWEEFKSFDKAAFAHRKSTMTSVALLESVPQWLYDYGLQELGSEWDEILKSLNKPAEV
                     FLRANTLRGTREELIKRLRAEGIEGMAVPEVESAVRLLTRKNVKMTEAFKQGYFEVQD
                     AASQLIAPLLHVEPGMKVVDACAGGGGKSLHLAALMKNKGKLICMDVHDWKLNDLKER
                     ANRNRVEIIETREIDSTKVIKRLESDVDRLLLDVPCSGLGVLRRNPDTKWKISLEEIE
                     KLQTTQAEIIETYSKMVKPGGMMVYATCSILPGENQNQVRKFLVNNNQDWQLVEQMHS
                     FPHREGFDGFYAALLRRKD"
BASE COUNT          835 a          688 c          574 g          829 t
ORIGIN      
        1 tcattggact tctggttgta ctcaaacaga agtaaagtcg cgctacaagt ttcaaaatga
       61 ataataagcg ccttcgcgac ttatgatagc cgtatcttcg tctagtagaa aatttaagac
      121 ccttctataa aacggagagc cccccatggg cctctccggc gaattcaaat aaaagagcca
      181 gaaatatctc ctggctcttt agtttaagct atatcaaaga tgcttatacg actattccat
      241 ttgcttcata gtattgtcgc cacctacgta gtagctctca gcttgttcag catgattcat
      301 gactgtatct acagaccatt tcatgaccat ttgcattcca gccactggtt gctctgatgt
      361 gcccatatta aatacgcttg gtacttctgc atttgcattg aaagtgtaac cccaagaaac
      421 atttagatcc gcaggcatga ttgtcgcgtt tgtgatgtat ttaccttggc ccttaagtgc
      481 gccgccagca gtaaaagtaa cacgataagc gaaatcaact acgtccatac cgtaaaggtt
      541 ttcgtattgt acacgataaa ctttagactg aggaatttgc cagccggtaa gatcagacca
      601 acattttaaa ccttgtggaa gggcattggc agtgtaagtt ttaatatgaa cagtaggacg
      661 tccggtttcc acaatggccc aaactttttt gccgatgtta acaattttat cgataataat
      721 gctgatttga tccaaaggat ctgcaggatt taagcttgga actccattat ctaatacttt
      781 cgcgcgtgaa gccggattgc aatcttcacc aagtccaggc tgtgtaaaag tctcagttaa
      841 agggtactga ttggtcactt cagtcacttt tacttttgcg agttggaaat attttacatc
      901 attttggtcg gcagcttgag cattgattgc aaaaacagtt gcaaatacta aaataatttt
      961 tgtcataggt tctcccctgt ttatttatgg aagattgttc taacaggggt atctattttt
     1021 tcaacctttt agtttttgcg tctgatactt agtgttagga tgtcatgaag aatctattag
     1081 ggacgccatg taaaactgat cattgggaag atccaatttt tttgagcagt gctgcttgat
     1141 tctacgctat cgacgacttt cttagaataa ccagcagcat aataatttaa actaaagcct
     1201 acataaagtt tgtcagcgat tggagcttgc accgcgaact gtccaaaata gctactcccc
     1261 gtccaagtct cgagagtgtt tccggagttg tactgaccct tggccaaata tccataagcc
     1321 agtgtcatac tgaatattcc tgccttatcg atattccacc gaagcgcagg accaatatcg
     1381 aaagaagaat atgttgccgt cgcgccatcc agggtgcttg tctgagaaat ccccaaaacg
     1441 ctccatccca cattccattg catacggcga tcaatgttaa aaagcacgtc cacattataa
     1501 aaatattgag tattggcagc ctcagaggac gcatttaatt tatccatgga atagattgca
     1561 ctgaaatcaa gatgggcttc ggcaaaggag actgaagaaa tcaaatggca aataagaaat
     1621 aaaagagcgg ctctcatccc ttatttatcg gctgtgcgaa aagattttag acaggacgag
     1681 agtccaaaat cagtccttac gcctcaggag tgcggcataa aatccatcaa agccctcgcg
     1741 atgaggaaag ctgtgcatct gctcgaccaa ctgccagtcc tggttattgt tgacgagaaa
     1801 tttccgcacc tgattttgat tttctccagg cagtatactg caagtggcgt aaaccatcat
     1861 gccaccaggt tttaccattt tcgaataagt ttcgataatt tcagcctgag ttgtttgaag
     1921 cttctcaatt tcctctaaag aaattttcca cttcgtatca ggattgcggc gaagcacccc
     1981 aagccccgaa cacggcacat ccaaaagcaa gcgatccaca tcagactcta gacgtttaat
     2041 cactttggta gaatcaatct cacgggtctc gataatttcc actcggttgc gattcgcgcg
     2101 ctcttttaaa tcattgagct tccaatcatg aacatccata cagatcaatt tgcccttatt
     2161 tttcataaga gctgcgaggt gaagactttt tcctccacca ccagcgcagg catcaacgac
     2221 cttcatcccg ggttcgacgt gaagcaaagg agcaattaat tgcgaagcgg catcttgaac
     2281 ttcaaaatag ccctgcttga aagcctcagt catctttaca ttttttcgcg tcaagagcct
     2341 tacggcggac tccacttcgg gaaccgccat tccctcgata ccttcagcac ggagtctttt
     2401 aatcaactcc tcacgagtgc cccgaagagt gtttgcgcgc agaaaaactt cagcaggttt
     2461 attcaaagat tttaaaattt cgtcccactc agaacctaat tcttgcaaac cataatcata
     2521 aagccattga ggaaccgact ctaaaagtgc aacagaagtc atggtgcttt tgcggtgtgc
     2581 aaaagcagcc ttatcgaaag atttaaactc ttcccactct gggagaatct ggtaagtatt
     2641 aaaacaccag gctgcccaca attgccaaac atcttctcga ctcatacttt tctcagtagg
     2701 ctcgaacccg gcaagccccc caaggtatcg ccaccagcgc accatctcat aaatgctctc
     2761 tgcgacgaaa cggcgatcgc gaccgcccca ttttttttca gatttcaaca ctcgctcaat
     2821 gaccttatcg gcgtaacgag cttgattgaa aatttcagtc accgcattaa caatattttc
     2881 cactaagtgg cgatgaaact ttatggatga gtcttgtgga cccgtt
//
LOCUS       DATBTE010000449         9600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_130637, whole genome shotgun sequence.
ACCESSION   DATBTE010000449 DATBTE010000000
VERSION     DATBTE010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9600
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130637"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1283)
                     /locus_tag="VIG33_17115"
     CDS             complement(189..1283)
                     /locus_tag="VIG33_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272617.1"
                     /translation="MRPQKILKTSLKAGMLFSIAGLMMGNQSCQETPVPQRELKRIIE
                     MGKMTSPSIELPQGGSFDFQYVANQQVYSVLQNSEHFALRYAPPVVDVPTAAKASGDS
                     KVLNVSSNDKVMMKAFAANAGLAEKPQYSTAAECLINLPNARIAGSVNAFEMIGGAAV
                     QIGFNAAGPLDISGLNGLGFSIEWAQLALSMMATHPLTDGLLAAANISSQQTKTKVHF
                     TFEVGPFMVGPSAYYQTPLAKVTEKALITGVTALYDGIKAKEEWYSRVLMDHDTHLVI
                     LGGANVKAKVGDQFNIYNELYYWEGDPCNSKYYGGGAASPVATIQLDWVGDEVSRGKI
                     LTQSDENPVLGAKVKILKLAPEPTPAKTTN"
     gene            1543..2955
                     /locus_tag="VIG33_17120"
     CDS             1543..2955
                     /locus_tag="VIG33_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY8272618.1"
                     /translation="MKSFGLKTKILLSTIPLLIATILVGLKIYQLGVVADPEIARALE
                     ISRIINQQEIEMVKMSDYLRGYILNPKDRRSYELKKEADERYAALSKELEPLVAEGPE
                     ISEINKKMANYDAIVLDEKEIQVALLVEKGDPGVQAYFANEYIPTRKVQEENFAKLKS
                     SAEEFSKKLVHEVERRKANSSYTTLTILAGSFAIGFVIIFLVLNSTVGIFMKSMKTLE
                     NTSLQMESTIINLNSQGQSLSGNSNSVASSLEETVASLEEMASMIKMNSENAQQAADL
                     SNKAQEVAVRGETEIKGLVQFMDEISHSSKKIEEIIGVIDDIAFQTNLLALNAAVEAA
                     RAGEQGKGFAVVADAVRTLAQKSSDAAKGISQLIKDSVDKMEKGAEVATESGTVLTEI
                     VQSIKKVSLLNDEISTASKEQSQGVTQLTQAMNQIDQSVQSNASATGEITSISEEVNA
                     HSSSLKVVVGELNHLIRASA"
     gene            complement(3001..5592)
                     /gene="clpB"
                     /locus_tag="VIG33_17125"
     CDS             complement(3001..5592)
                     /gene="clpB"
                     /locus_tag="VIG33_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEY8272619.1"
                     /translation="MSNEIEKMTRKSQEAMQAAAQNAENRGNPSVEPEHLLLELLQQS
                     EGIVPRIFEKQNLSVKSMIDDLKTKIDKFPRVTGGGQKVFASQRLQKLFTVADKVSLD
                     WGDAYISTEHFLLGLFGLDDNDINAFFKRHQIKHDPMVQALRELRGNQKVTDDNPENK
                     YEVLNKYARDLTALAAEGKLDPVVGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAI
                     AEGLAVRILKQDVPENIIGKKLMSLDMGALIAGAKYRGEFEDRLKAVIKEVTASNGQI
                     ILFIDEIHNLVGAGKTEGAMDAGQLLKPALARGELRCIGATTLDEYRKYIEKDAALER
                     RFQTVLVDEPSVEDAITILRGLKEKYELHHGIRIMDSALVAAVKLSHRYIQNRFLPDK
                     AIDLMDEAASKLSIETKSVPEEIDKIERELMQLRIEKEALKKEKEEGARERLQVIEKE
                     IKELTAKNQQLREQWNFEKGGIEQIKKTKAEIDEIRNLIGKAEREGDLGKAAELKYGR
                     LPEMEKKLKEFEARGNKTVNEARMLKEEVSPEDVAEVVGKWTGIPVSKMLESESQKLL
                     HMEEDLQKRVVGQDHALVVVADAIRRARAEISDPNRPIGTFIFLGPTGVGKTETVKAL
                     AEFLFDDEQAVVRIDMSEYMEKHSVSRLVGAPPGYVGYEEGGQLTESVRRKPYSVVLL
                     DEVEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVLIMTSNIGSHALIDPNMSEAE
                     KQKAVNEAMRTHFRPEFLNRIDEVVMFKALGEAQIAGIVKVQLNLVKERVKAKKINID
                     FTDDAVQLLARKGYDPIYGARPLKRVIQNDLLNPLSKAIIAGDVRSGDSIIIEVRGQG
                     LNIVKKS"
     gene            5641..5895
                     /locus_tag="VIG33_17130"
     CDS             5641..5895
                     /locus_tag="VIG33_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272620.1"
                     /translation="MPSEKPSKKIPTKRIHPSIKKDGVNPSDFLKYDFRTSCEDCSHF
                     DSLKNLCTLGYNPAPHLKAQQLRDYEASGKIAQCRFLEVD"
     gene            6335..7168
                     /locus_tag="VIG33_17135"
     CDS             6335..7168
                     /locus_tag="VIG33_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY8272621.1"
                     /translation="MGMRVTTNIGALNAQRNLIGAQRTLQGSMAQLASGSRINKAADD
                     AAGLAISENLKAQIRSATQAQRNANDGISMVQTAEGGLNEIGNIVVRLRELGIQAASD
                     TVGNTERGFVDKEVQQLKAEIQRIASVTTWGTTKLLDGSSPKFDFQVGLFNNQEQDRI
                     TFNADENVATLDALGLAGLDYTSKEGAQESLMLLDAAQTSVNGTRANLGALQNRLTST
                     VDNLGVAQENMSAANSRIRDTDVAQASSEMTRNNILLQAGTSTLAQANQVNQLALKLI
                     G"
     gene            7390..8139
                     /locus_tag="VIG33_17140"
     CDS             7390..8139
                     /locus_tag="VIG33_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272622.1"
                     /translation="MLKKTIIKIFTLGCTLITLLACSGKGGFDIQGSPSSGGNNSGST
                     DNNGSSGSNGAIDPDAFQKLQYKGLISGGNNDGTLVVDIDKVNGALLLIVPLQMNPYI
                     EPAQGEIPDLPGVKFMTYKDDKGASYFAVNVPLRYVRKGASFLNPARLPNGDALPQVA
                     SGELPSVAVAVPGKNNVKFNFYIGVNVIGVYISSPYDPYMALHFPIRKGVETIGYFHT
                     VPAKPGFEGGFFLNTQMPDSVAAIIDDHFRF"
     gene            8252..8752
                     /locus_tag="VIG33_17145"
     CDS             8252..8752
                     /locus_tag="VIG33_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272623.1"
                     /translation="MAKKTSKTKKATKKAIKKVAPKKATKKSAAKKTTKKTAPKKASK
                     KVAPKKAATKKTAPKKAAKKTTAKKAAPKAKAKKTTFVGETSPVKPETALDNIAKEFD
                     DETELDSMIDIEEEPLSEDEESWEDTEEDKEEDSFEDLEDKTNKKIEDEDEEDDEDED
                     DEEGYF"
     gene            complement(8848..>9600)
                     /locus_tag="VIG33_17150"
     CDS             complement(8848..>9600)
                     /locus_tag="VIG33_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8272624.1"
                     /translation="ELFGHEKGSFTGAYNKKIGLAEAASGGTLFLDEIGELSPGIQAK
                     LLRFIQEGELYRVGGKDAIKVDIRLISATNRELDAEVLRGNFREDLFYRINTIVVSAP
                     PLRRRREDIAALVTHFLSNGNHSYLNRGRQISEEALKTLQKYDWPGNIRELQNVCERL
                     QILSEGHTIMINDLPENIRNPEKADDIIDYDPGLTLHDLEKRYILKALAHFGGNKTQA
                     ANNLGITIKTLYNKLHEYGEFEKFAVHSKPMK"
BASE COUNT         2745 a         2232 c         1981 g         2642 t
ORIGIN      
        1 aggcattgac tagcgcctgc gaacttttga tttcactgac agcctcgatg atccctgaaa
       61 acataaaaaa gatccccttg atagggcgga aactacactc tttacagaaa cttggcaaga
      121 aaagggggct ttttagagcc cccttttaaa tgctttttta atttttttac ttcagaatgt
      181 cttaaatctt agttcgtggt tttcgcgggc gtaggttcag gagctaactt caagattttc
      241 acttttgccc ccaagacagg attttcatcg ctttgagtga ggatcttgcc gcgggaaact
      301 tcatcaccga cccaatccag ctgaattgtt gccacagggc ttgccgcgcc accaccgtaa
      361 tatttggagt tacatgggtc tccttcccaa taataaagtt cattatagat attaaactgg
      421 tccccgacct ttgcttttac atttgctcca cccaaaataa ccaaatgcgt gtcatgatcc
      481 ataagtacgc gggaatacca ttcttctttc gccttaatac catcatagag agcagtcact
      541 cctgtgatca aagccttctc ggtcactttc gcgaggggag tttgatagta cgcgctcggt
      601 cctaccataa aaggacccac ttcaaaagtg aagtgcactt tggtttttgt ttgctgtgaa
      661 cttatgtttg cggccgccaa aaggccgtcc gttaaaggat gcgtagccat catactcaag
      721 gccaactgag cccattcaat actaaacccc aggccattga gaccggatat gtccaaaggc
      781 cctgccgcat tgaaaccaat ttgaactgca gcgcccccaa tcatttcaaa ggcattcacc
      841 gagccagcaa tccgcgcgtt tggcagattg atcaaacact cagccgctgt cgaatattga
      901 ggtttttctg ccagccccgc attcgccgca aaggctttca tcataacctt gtcattagaa
      961 gagacattca gcactttcga atctcccgat gccttcgcag ctgtcggaac atctacgaca
     1021 gggggagcat atcttaaggc gaagtgttcg ctgttttgca aaacactata gacttgttga
     1081 ttggctacat attgaaaatc gaaactgcca ccttgcggaa gctcaatact gggcgacgtc
     1141 atcttgccca tttctatgat acgtttaagc tcccgttgag gcactggagt ttcctggcat
     1201 gattgatttc ccatcatcaa acctgctatt gaaaacaaca tcccagcctt tagtgaagtt
     1261 tttaaaatct tttgcggtct catctgtcct ccagttaaat cgttccgtac ctcctttcgg
     1321 ctggacttta gcccgagtta agctttttat acaccaaagt cgagtcctca gttgaaccac
     1381 cgtgaaatcg tcaaaaagtt tttctgattc ttcaaaagtt agaactgaat caggctcacg
     1441 agatcgagat ttgaaaaaat cgatcatatt tttatcacaa gagtcgagat ggttaagtcc
     1501 tttttaatcc aacccgatat aaatgtatct aaggaatcat ctatgaaatc atttggactt
     1561 aaaacgaaaa ttcttctatc gacaatcccc ctcctcattg caacgatctt ggtgggtctt
     1621 aaaatctatc aattaggggt tgttgccgat ccggaaatcg cacgagcttt agaaatctct
     1681 cgaattatca atcaacaaga gattgagatg gtaaagatgt ccgattactt aagaggttat
     1741 attttaaatc ccaaagacag aaggagctat gagctaaaaa aggaagctga cgaaaggtac
     1801 gccgcccttt cgaaagagct agaacctttg gtggcggaag gccctgaaat ctcagagatc
     1861 aataagaaga tggcaaacta tgacgccatt gttttagatg aaaaagaaat acaggtggcc
     1921 ctcctcgttg aaaaaggcga tccgggggtt caagcttatt ttgctaatga gtatattccc
     1981 acgcgcaaag tccaagaaga aaattttgct aagctaaaaa gctctgcgga agaattctca
     2041 aaaaaattag ttcatgaagt ggagaggcga aaagcaaatt ccagctatac taccctgaca
     2101 atccttgctg gtagctttgc aatcggcttc gtcattattt tcttggtgct taactctacc
     2161 gtggggatct tcatgaaatc gatgaaaacc cttgaaaaca ccagcctaca aatggagtcc
     2221 acaattatca atctcaattc ccaaggacag agcctttcag gaaactctaa ctccgtagca
     2281 tcttcgctgg aagaaaccgt ggcctcactg gaagaaatgg cctctatgat taagatgaac
     2341 tctgaaaatg cccagcaggc cgccgatctc tctaataaag cgcaagaagt ggcggtgcgc
     2401 ggagaaacgg aaattaaagg cttggtgcaa tttatggatg aaatctcgca ctcatctaaa
     2461 aaaattgagg agatcattgg cgtaatcgac gatatcgcct ttcaaaccaa tttactggct
     2521 ctgaatgcag ccgtggaggc cgctcgcgct ggcgagcaag gcaaaggttt tgctgtcgtc
     2581 gctgacgccg taagaacgtt agcccaaaaa agctcggatg cagctaaggg catttcccaa
     2641 ttgattaaag attccgtaga taaaatggaa aaaggagccg aggtcgctac agaaagtgga
     2701 acggttttaa ccgaaatagt tcaatccatc aaaaaagtaa gcctactcaa tgatgaaatc
     2761 tcaaccgcca gcaaagaaca gtctcaaggt gttactcaat taactcaagc tatgaatcaa
     2821 atcgatcaat cggttcaatc taacgcttct gcaacagggg aaatcacttc catatctgag
     2881 gaagttaacg ctcatagctc cagtttaaaa gtagtggtag gggaactcaa tcacttaatc
     2941 agagcctctg cttaagagac tcttaagcag agatccttca tcagtggctt tgatgctgag
     3001 ttaagatttt tttacaatat tgaggccctg tccacgcacc tcaataatta tgctgtctcc
     3061 cgagcgcacg tctccagcta tgatagcctt agaaagcgga tttaaaagat cattctgaat
     3121 aacccgcttt aaaggtctgg caccgtagat agggtcataa ccttttctag ctaaaagctg
     3181 aaccgcatcg tccgtgaagt cgatgttgat cttctttgct ttcacgcgtt cttttactag
     3241 gttcaattgc actttaacaa tgcctgcaat ctgggcctcg ccaagtgcct tgaacatcac
     3301 gacttcatcg atccgattta aaaactcagg tcggaaatga gtccgcatgg cttcattaac
     3361 cgctttctgc ttttctgcct cgctcatgtt ggggtcaatt aaagcgtgag agccaatatt
     3421 agaagtcata atcagcacag tatttttaaa atccactgtg cgaccttgac cgtcggtcag
     3481 acgaccatcg tctagcacct gcaatagaat attaaacacg tccggatggg ctttctcgac
     3541 ttcatcaagt agaaccacac tgtaaggttt gcgacgaacg gactctgtca attgtccgcc
     3601 ctcttcgtag cccacatatc ctggaggagc ccctacgagt cttgagaccg agtgcttttc
     3661 catgtattca ctcatatcaa tgcggaccac cgcttgctca tcgtcgaaga ggaactcggc
     3721 caaagccttg actgtttccg tttttcccac cccggtaggg cctaagaaaa taaaggtccc
     3781 aatagggcga tttggatcgg aaatctccgc acgtgcacga cgaatagcat cggctacgac
     3841 aaccagcgca tgatcttggc cgaccacgcg tttttgaagg tcttcttcca tatgcaggag
     3901 cttttgagat tcactttcca gcatcttgga aaccggaata ccggtccact tgcccacaac
     3961 ctctgccaca tcctccgggc tcacttcttc tttaagcatg cgcgcttcat taacagtttt
     4021 attgccgcgg gcctcaaatt ctttgagctt cttctccatc tcaggcaatc gaccgtattt
     4081 caattccgct gccttaccaa gatccccttc gcgctcggcc ttgccaatga gatttctgat
     4141 ctcgtcaatt tcagccttgg ttttttttat ttgttcaata ccaccttttt cgaagttcca
     4201 ttgttcacgc agttgctggt tcttcgcagt gagttctttg atctcttttt ctatgacttg
     4261 aaggcgttct ctggccccct cttccttttc tttttttaat gcttcttttt caattcgaag
     4321 ctgcatgagc tcacgttcaa ttttatcgat ttcttccggc acgctcttgg tttcaatact
     4381 gagcttgctg gcggcctcgt ccatcaagtc gatggcttta tccggcaaaa acctattttg
     4441 aatataacga tgcgaaagct tcacagctgc caccaaagcg gaatccatga tccgaattcc
     4501 gtgatgcaat tcgtactttt ctttaagccc gcgcagaatg gtaatcgcat cctcgacact
     4561 aggctcgtcc actaagaccg tttgaaaacg gcgctcaagg gcagcgtctt tttcaatata
     4621 cttgcgatat tcgtcgaggg tggtcgcgcc gatgcaacgg agttcaccgc gagccagagc
     4681 tggtttcaat aattggccag catccattgc cccttctgtt tttcccgctc cgacaagatt
     4741 atgtatttca tcgataaaga gaatgatttg tccattactt gcggtgacct ctttaatcac
     4801 agctttcaaa cggtcttcga attctccgcg atatttcgcg ccagcaatca aagctcccat
     4861 atctaaagac atgagtttct tacctatgat attctccgga acatcttgct taaggatcct
     4921 taccgccagg ccttccgcaa tggccgtttt ccccacccct ggctccccga tcagaactgg
     4981 attgtttttg gtgcggcggg aaagaacctg tatcactcga cggatttctt cgtcgcgacc
     5041 gaccacaggg tccaattttc cttccgccgc cagcgccgtc aaatcccggg catatttatt
     5101 taaaacttcg tatttgtttt cgggattgtc gtctgtcact ttttgattcc ctcgcaactc
     5161 tcggagagct tgcaccattg ggtcatgttt aatttgatgt cttttaaaga aggcattgat
     5221 gtcattatcg tctaaaccaa aaagcccgag gaggaaatgc tcagtagaga tgtaagcatc
     5281 cccccaatcc aaagaaacct tgtcagccac ggtaaaaagc ttttgcaatc tttgactggc
     5341 aaagaccttt tgtcctccac cagtgacgcg cggaaattta tcaattttcg tctttaaatc
     5401 atctatcatg gatttaacgg agagattttg cttttcaaaa attctgggaa caataccttc
     5461 tgattgctga agtaattcta gaagaaggtg ttcgggttct actgaaggat ttccccgatt
     5521 ttcagcgttt tgggcagccg cttgcatggc ttcctgactt ttacgcgtca ttttttcaat
     5581 ctcattgctc ataaactcct ccgtcctgta ttaagaatga gtcttcagga gagattgtca
     5641 atgcccagcg aaaaacctag taaaaaaatt ccaacaaaac gcatacatcc ttctattaaa
     5701 aaggatgggg tcaacccttc tgatttttta aagtatgact tccgtacatc ttgtgaggat
     5761 tgctctcact ttgattcttt aaaaaatctt tgcactttag gatacaatcc agctccgcat
     5821 ctcaaagcac aacaactgcg tgattatgaa gcctccggaa aaatagctca gtgccgattt
     5881 cttgaagtcg attaatttca aataggttaa ttttgactga accaatgtcg aggcttagtc
     5941 ctgaatgtta tcctttggta ctgtaacaaa gcagcgcaag accatccctt gggcttgagt
     6001 tccgaaaact ttcctcttct atccttagac tagtattgca taaaaaacgc aaatcccgcc
     6061 tacagtcctg tcaagacttc gtcacagatc ccgatcttaa acacatcaac gttagaagga
     6121 taggagagag ttcaaggtca ctctccctag aacacgaaag gagggccttc aaggatgaac
     6181 taagaatgaa caatcgaatg ttctttactt tactaaatag taataggcca aaacataata
     6241 ggctcggatg ccaccccaca gacaagatga ggggaataaa actcaaggag ggtacttaaa
     6301 tgaaaattaa taattcttaa cggaggattt taaaatggga atgagagtaa caacaaacat
     6361 tggtgcctta aatgcacaga ggaacttgat cggggcacaa agaaccctcc aaggctccat
     6421 ggcacaactg gcttcgggaa gccgcattaa caaagctgct gacgatgctg cgggtctagc
     6481 gatttctgaa aacttgaagg cgcaaattag atctgcaact caggcacaaa gaaatgcgaa
     6541 cgacggtatc tcgatggttc aaaccgctga gggcggcttg aatgaaatcg gtaacatcgt
     6601 ggttcgtctt cgtgagctcg gaattcaggc tgcatctgac acagttggaa atactgagcg
     6661 cggtttcgtg gataaagagg ttcaacaatt aaaagctgaa atccaacgta tcgcttcggt
     6721 tactacttgg ggaacaacca aattattaga cggttcttca cctaagttcg acttccaagt
     6781 tggtcttttc aataatcaag aacaagatcg catcactttc aatgccgatg agaatgtggc
     6841 aactctcgat gctttgggat tggctggtct tgactatacc tctaaagaag gtgctcaaga
     6901 gtccttgatg ttgcttgatg cggctcaaac ttcagtgaat ggaactcgcg ctaatctggg
     6961 tgcccttcaa aatcgtttga cctcgacagt tgacaacttg ggcgttgctc aagagaacat
     7021 gtcggctgcg aacagtcgta tccgtgatac agatgtggcc caggcttctt ccgaaatgac
     7081 tcgtaataat atcttacttc aagctggtac ttctactcta gctcaagcaa accaagtaaa
     7141 ccaattggcg cttaagttga tcggttaagc tatcagttgc gcaaagctaa aacaaaacct
     7201 ggtgggttcc ctgccaggtt ttttcatttt tgcagatgtt attgacataa gcctgaccta
     7261 aaactgtctt ttctaatttt ctgttaaact actttactaa taaaatcaaa tatttagctt
     7321 aagctataca gactttcatc cgaaaacaag ggagagataa agtaaaaggg aaaaaggtct
     7381 ggagaagata tgttaaaaaa aaccattatc aaaattttca cgcttggatg tacgcttatc
     7441 accctcctcg cctgcagtgg caagggggga tttgacatcc aaggctctcc cagcagtggt
     7501 ggcaataact ctggctcaac ggataataat ggaagctcag gctccaacgg cgcaatagat
     7561 cctgatgctt ttcagaagct ccagtacaaa ggccttatct ctggcggcaa caatgatggg
     7621 actctggtgg ttgatatcga taaggtgaac ggagccctcc tcttgattgt tccactacaa
     7681 atgaatcctt atattgaacc tgcgcaggga gagattccag accttccggg ggttaaattc
     7741 atgacttata aagatgataa gggtgcttct tacttcgccg tcaacgttcc tttacgctat
     7801 gttcgcaaag gagcaagttt tttaaatccc gctcgtttgc caaatggcga cgctcttcct
     7861 caagtcgcca gtggagaact cccaagcgtg gcggttgcgg ttccgggtaa aaataatgtg
     7921 aagtttaact tctatattgg agtgaatgtg atcggagtct atatttccag cccttatgat
     7981 ccttatatgg cccttcactt ccctatccgt aagggtgtag aaacgatcgg atattttcat
     8041 acggtccccg caaaacccgg tttcgagggt ggcttctttt tgaatactca aatgccagac
     8101 tctgtagccg cgattattga cgatcacttt cggttctgat gaccaagtcg ttgatctaaa
     8161 gaagtaatta attattgatt gccccaaaaa aaatcgttaa tctctttgct gtatgctcta
     8221 cgcgatgtgc tttaacttct aagggggcct catggcaaaa aaaacatcta agactaagaa
     8281 agctactaaa aaagcaatta aaaaggtagc tcccaaaaaa gctaccaaaa aatctgcagc
     8341 taaaaaaacg actaagaaga ccgctccgaa gaaggcttct aaaaaagtag ctcccaaaaa
     8401 agctgctact aaaaaaaccg ccccgaaaaa agcggccaaa aaaaccactg ctaaaaaggc
     8461 cgctccaaaa gctaaagcga aaaaaacgac ctttgttggt gaaacctctc ctgtaaaacc
     8521 tgagacagct ttagacaata tcgctaaaga attcgacgat gagacagaac tcgatagcat
     8581 gattgatatc gaagaagagc ctcttagcga agacgaagaa agctgggaag acaccgaaga
     8641 agataaagaa gaagactctt ttgaagactt ggaagacaag actaacaaaa agattgaaga
     8701 tgaggatgaa gaagacgacg aagatgaaga tgacgaagaa ggttacttct aatccagatt
     8761 tagacttaaa tttaagctca aattaaatta aaatggaaaa aggggctgtc gaaactacag
     8821 ccctttgttt tgtctgaata ctcaagttca cttcatcggt ttagagtgaa cagcgaactt
     8881 ttcaaattca ccgtattcat ggagtttatt ataaagagtt ttaatcgtaa tccccaggtt
     8941 attagccgct tgggttttat ttcccccaaa atgggccaaa gctttcaaaa tatatcgttt
     9001 ttccagatca tgaagcgtca agcctggatc ataatcaatg atatcatcag ctttttcagg
     9061 gttacgaatg ttctccggca gatcatttat catgatcgtg tggccttctg ataaaatttg
     9121 taatctttcg caaacatttt gaagctcacg gatattgccg ggccagtcat atttctgaag
     9181 agtcttcaaa gcctcttcag agatctggcg tccacggttc aaataagaat gatttccgtt
     9241 gctcaaaaaa tgagtgacca aagcagcaat atcctcacgg cgacggcgca gaggcggagc
     9301 gctgaccaca atagtattaa tacgataaaa cagatcttca cggaagttac cgcgcagaac
     9361 ttctgcatcc aattcccgat tggtagccga aattaaacgg atatccactt tgatagcgtc
     9421 cttacctccg actcgataca gttcaccttc ttgaataaag cggagaagtt tggcctgaat
     9481 accagggctt agctccccga tttcatctaa gaataaagtt ccaccactcg cggcttctgc
     9541 taaaccaatc tttttattat atgcgcctgt aaaagaccct ttttcatgtc caaaaagctc
//
LOCUS       DATBTE010000450         5892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_948616, whole genome shotgun sequence.
ACCESSION   DATBTE010000450 DATBTE010000000
VERSION     DATBTE010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948616"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /gene="rpsI"
                     /locus_tag="VIG33_17155"
     CDS             complement(<1..350)
                     /gene="rpsI"
                     /locus_tag="VIG33_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY8272625.1"
                     /translation="MAAEKFYYATGRRKTSSARVFLKPGKGTITINGKKANDYLKRMQ
                     SRMVILQPFETVDQTDKFDVRITVSGGGENGQAGAVRHGITRALVAFNPEFKGVLKKA
                     GFVTRDPRMVERKK"
     gene            complement(365..811)
                     /gene="rplM"
                     /locus_tag="VIG33_17160"
     CDS             complement(365..811)
                     /gene="rplM"
                     /locus_tag="VIG33_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY8272626.1"
                     /translation="MMKTWNAKAEEVERKWHIIDASGLKVGRLATHVATILRGKNKPI
                     YSPNVDTGDFVVVINTDKAILTGNKANDKKYFRHSRFFGSLKEKTAAQMQAEDSTFII
                     HEAVRGMLPTNKLSRQVISKLKIYTGGEHPHSAQKPAVYNIPTGKI"
     gene            complement(893..1726)
                     /gene="truA"
                     /locus_tag="VIG33_17165"
     CDS             complement(893..1726)
                     /gene="truA"
                     /locus_tag="VIG33_17165"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY8272627.1"
                     /translation="MESALTSELSSGITASMQRIRFLVSYDGTDFCGWQKQNHMPNKS
                     VQQTLEEVLTGIFQEKITLFASGRTDAGVHAVGQVCHFDTERPVERLQNWDLAWAMKS
                     RLPRSISVKKVWLAPPDFHATLSAERKTYRYLIYNKMRAGAFLTRYADWARHPISIQH
                     LQESAKFLLGKQDFKSFQSVGSPVTHTVREIYQADWQWRKSDLLQFTITGNGFMKQMV
                     RNIVGTQMMLEKKGLAPETLRDIIAVQDRKQAGPAAPPEGLFLMRVYYPQELDIRCRE
                     I"
     gene            complement(1704..2552)
                     /locus_tag="VIG33_17170"
     CDS             complement(1704..2552)
                     /locus_tag="VIG33_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272628.1"
                     /translation="MKFFTVTTLMTAVAVFISQAHAVNKEAPPMEPPYTNESEFDPVG
                     GEKSREEKTPQKGVKWIHHPDAKKGLIRIEQDGTYVYKIKTTKKNQTGIVRFGFMSPP
                     DIKSADGLSDFKSMYTSSEVPTIMFDYEWQPFSKYGKLGLQVGMGVGTANGQGHFLKD
                     GSIAPEKYTFVILPLNVGLLYRLEYFNRQWVAPYVAGGGTYIAVAELRDDGKQHFSGT
                     PAAYAGGGLMFNITALDKSMAFNLDSEYGVGNLWFVAEYRLQTSFSEDLNFRGSIMSF
                     GISADF"
     gene            complement(2549..3997)
                     /locus_tag="VIG33_17175"
     CDS             complement(2549..3997)
                     /locus_tag="VIG33_17175"
                     /inference="COORDINATES: protein motif:HMM:NF041770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HEY8272629.1"
                     /translation="MEITRTLISNLILRACLFLTGSISGYFAVAQTTTPTPSGSTTAM
                     EIVGSPGGVSAYDAGTAAAGTTAATPPTIYGGYTGATGECAASATLLNSATCNSCDGS
                     GRVTCNQTSIHPDLYLTVTLRSSTASSFTGTPRVRWKFSGELAGHAIDNTAAPSLVAG
                     TPFTVQIKWSNLCSYAGGNSSCTADIDASKTLSIGIDTNNDDATIEEKVDFNFIFRYL
                     QPASSTNTLTACPVGTPGLVNQGICDYTVAAGDEKVVITDYATADNSLKTPNTSVTYN
                     RIIMFYQKGVGNASTVTNKSDYLILNLINNSPAEPSISDQRITGLENDVKYCFALGNM
                     DQTGIISYFPPDSVLTNSAQVCATPSQVVGLLDDKHCFIATATYGSDMAPEVQTFRKF
                     RNEFLLKNFVGRYLVKFYYKFGPEAAEWISSSEILKAVSLGILWPLLMFVKLSLFLGM
                     IPAIIVGLISILLLKRAFPLLFQGFKALKGDA"
     gene            complement(4010..5032)
                     /locus_tag="VIG33_17180"
     CDS             complement(4010..5032)
                     /locus_tag="VIG33_17180"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEY8272630.1"
                     /translation="MIKNFKVGVVGATGMVGQTFMNILEERKFPLMELRPFASEASVG
                     KKIHVQGQDWSVQTLAPDCFEGLDLVFFSSGDDISKEWAPQAAKAGAFAIDNSAAFRM
                     DPKVHLIVPEVNGHLVNSHSKPQVIANPNCSTIQLVVALKPLADKYGLEEVRVSSYQA
                     VSGAGLAGYEELLAQTEDYKNPKPKPTTFPHPILFNCIPQIGSFNEEGFCSEEVKIMK
                     ETRKILSLPELKVSAFTVRIPALNAHSESVWVTLKQKTDQANILETLKNFEGIVVQDE
                     TQNSIYPTPRSVSGEDPVYVGRVHRDPENPYLWLMWVVSDNIRKGAALNGLQIAEHIF
                     DIKPQA"
     gene            complement(5016..5867)
                     /gene="pssA"
                     /locus_tag="VIG33_17185"
     CDS             complement(5016..5867)
                     /gene="pssA"
                     /locus_tag="VIG33_17185"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEY8272631.1"
                     /translation="MADNHSSSENVEPAGKKLAIYIYVLPNLMTTGNLFCGFFAIIQA
                     LKSNFLYGAYAIVAAAVFDLLDGRLARLTRSTSKFGAEYDSLCDLVSFGVAPGIMLYQ
                     WALNPFGRLGWVACFLFVTCGALRLARFNVQANIVEKNYFQGLPIPMAAGIVASSILA
                     FQDLELEASGNIGLLVMTALLAFVMVSNFRYRSFKDLDLKERMPFHYLLLGVGVLVVV
                     ALYPEVMLFVLFFGYAVLGAVFGVFKLGKNVRKIRPSVYLPAQVNEDDLVEEDEETGS
                     PKHDKKL"
BASE COUNT         1684 a         1376 c         1244 g         1588 t
ORIGIN      
        1 tatttctttc tctcaaccat acgtggatcg cgggttacga agccagcttt tttcaaaaca
       61 cccttgaatt cagggttaaa tgctacaagt gcacgagtga ttccatgtct taccgcgccg
      121 gcttgaccgt tttcaccgcc accagaaact gtgattctta cgtcaaactt gtcagtttga
      181 tctacagttt caaagggttg aaggataacc attcgagatt gcattctttt aaggtaatca
      241 ttagcttttt tgccgttgat tgtgattgtg ccttttccag gcttcaagaa aacacgtgct
      301 gagctcgttt ttcttcttcc agtagcgtag taaaattttt ctgctgccat tgactatgtc
      361 cttcttagat cttgccagta ggaatgttat atacggctgg tttttgagct gaatgaggat
      421 gctcaccacc agtgtagatt ttcaatttag agatgacttg gcgagaaagc ttgtttgtag
      481 gaagcattcc gcgaaccgct tcatgaatga taaaagtaga gtcttcagct tgcatttgag
      541 ctgcagtttt ctctttcaaa gatccgaaga aacgagagtg tctaaaatat tttttgtcat
      601 ttgctttgtt gccggtcaat attgctttat cagtattgat gacaacaacg aaatcgccgg
      661 tatctacgtt aggggaataa ataggcttat ttttgccgcg tagaatagtt gctacgtgag
      721 tagccaaacg acctacttta aggccgctgg catcgataat atgccatttt cgttcaactt
      781 cttcggcttt tgcattccaa gtcttcatga tgactccaat taaatctcta tcaaacgctt
      841 taagaggcat agtagatact tcctttgcct caaaactgca agaagggatg ttttaaattt
      901 cgcggcacct aatgtcaagt tcttgaggat aataaacgcg cattaaaaag agcccttctg
      961 gcggagccgc cggcccagct tgtttgcggt cctgaactgc aataatatcg cgaagagttt
     1021 ccggggctaa gccctttttc tccaacatca tttgcgtccc aactatatta cggaccatct
     1081 gtttcataaa cccattaccg gttatagtaa attgaagcag atcgctttta cgccactgcc
     1141 aatcagcctg ataaatctca cgaaccgtgt gggtcaccgg agagcccacg gattgaaaac
     1201 tcttaaagtc ttgttttccc aaaagaaact tcgcgctttc ttggaggtgc tgaatactaa
     1261 tagggtgcct tgcccaatcc gcataacggg tcagaaaagc tcctgctcgc atcttattat
     1321 agatcaaata acggtaggtt tttcgttcgg cggataaagt cgcatgaaaa tctggcggcg
     1381 ctaaccatac ttttttaact gagatagatc gtgggagacg ggatttcatg gcccatgcga
     1441 gatcccagtt ttgcaacctc tccactggac gttccgtgtc aaagtggcag acttgcccga
     1501 cggcatgaac tccagcatcg gttcgtccac tcgcaaacaa ggtaattttt tcctgaaaaa
     1561 tgcccgtgag cacctcttca agggtttgtt ggacactttt attgggcata tggttctgtt
     1621 tctgccagcc acaaaaatca gtgccatcat aagaaactaa aaaacgaatc ctctgcatag
     1681 aggcggtaat accagaactc aactcagaag tcagcgctga ttccaaaact catgatactc
     1741 cccctgaagt tgagatcttc agagaaagag gtttgtaaac gatactcagc gacgaaccaa
     1801 agattgccca ccccatattc actatctaaa ttaaaggcca tgctcttatc aagagccgtg
     1861 atattaaaca tcaaaccacc ccccgcataa gccgcaggag ttcccgaaaa gtgctgtttt
     1921 ccatcatccc gtaattccgc caccgcgata taagtcccac cgcccgcgac ataaggagct
     1981 acccattgac ggttaaaata ttcaagacga tacaataatc ccacatttaa agggagaatg
     2041 acgaaggtgt atttttcagg agcgatcgaa ccgtctttaa gaaaatggcc ttggccattg
     2101 gctgttccga ctcccatccc cacctgtaag ccgagttttc cgtacttaga gaagggctgc
     2161 cattcgtaat caaacataat ggttggcact tcgctgctgg tatacattga tttgaagtcc
     2221 gataggccat cagcagattt gatatccggc ggggacataa acccaaaacg cacaatacct
     2281 gtttgatttt ttttagtggt cttaatttta taaacatagg tgccatcttg ctcaatgcgg
     2341 atcaacccct tcttcgcatc ggggtgatga atccacttta cacctttttg tggcgttttc
     2401 tcttcacgcg atttttcgcc gcccaccgga tcaaactcag actcattcgt ataagggggc
     2461 tccataggtg gagcttcttt atttaccgca tgagcttgag aaataaagac agccaccgcc
     2521 gtcatcaaag tggtcacggt gaagaacttc acgcatcccc cttcaaagct ttaaaacctt
     2581 gaaagagtaa ggggaaggct ctcttcaaca gtaagataga aatcaaacct actataattg
     2641 ctggaatcat tcctaaaaat aaactgagtt ttacaaacat taaaaggggc caaagaattc
     2701 ctaaactgac agctttcaag atctcgctgc tagaaatcca ttccgcggct tcgggtccga
     2761 atttgtaata aaacttaacc agatacctgc cgacaaaatt cttgagcaaa aattcattgc
     2821 gaaactttct aaaagtttgc acttcagggg ccatgtcact gccataggtc gcggtggcaa
     2881 taaaacaatg cttatcatcg agaagaccca ccacttggga aggagtggca cagacttgag
     2941 cggagtttgt taaaaccgag tccggtggaa aataagaaat gatgccggtt tggtccatgt
     3001 ttcctaaagc aaaacagtac ttcacatcgt tctcgagacc agtgattctt tgatcggata
     3061 tacttggctc agccggagaa ttatttatta aattcaaaat taaataatct gatttatttg
     3121 tcaccgtgga cgcattccct acgccctttt gataaaacat gatgatacga ttgtaagtta
     3181 cagaggtatt gggcgttttc agggagttat cggctgttgc ataatctgta atgacaactt
     3241 tttcatctcc tgcggccacg gtataatcac aaattccctg attgaccaac cctggcgtac
     3301 cgactggaca tgcggtgagg gtatttgtgg aacttgcggg ttgcaaatag cgaaaaataa
     3361 aattgaaatc gaccttctct tcgatggtcg catcatcatt atttgtatca attcctatac
     3421 tcaaagtctt gcttgcgtct atatcggcgg tgcaagagga attaccccct gcatagctac
     3481 acaggttaga ccatttaatt tgcaccgtaa aaggggtgcc tgcaacaagg cttggagcag
     3541 ctgtgttatc aatcgcatgc cccgcaagct ccccactgaa tttccagcga actcgcggag
     3601 tgcctgtaaa actgcttgcc gtactcgatc tcaaagtcac ggttaaatac aaatctggat
     3661 gaattgaagt ctgattacaa gttactcgac cagagccatc acaggaattg caggttgcgg
     3721 aattcaagag ggtggctgaa gcggcgcact ctccagtagc gccggtatat cccccataaa
     3781 tagtgggagg cgttgccgcc gttgttcctg cggccgctgt gcccgcatca tatgctgaga
     3841 ctccgcctgg agatcctaca atttccatag cggttgttga accagaagga gttggtgttg
     3901 tcgtctgagc gacggcaaag taacccgaaa tgctgccagt aaggaataag caggctctta
     3961 aaataagatt tgaaataagc gtccgtgtta tttccaccta tttatgacat catgcctggg
     4021 gcttgatgtc aaaaatatgc tctgcgattt gaaggccgtt caaagcagcc cctttgcgta
     4081 tattgtccga caccacccac ataagccata gataagggtt ctctggatcc ctatgaacgc
     4141 gtccaacgta aacagggtct tcccctgaca cgctgcgagg agtaggatag atagaattct
     4201 gtgtctcatc ttgaaccaca attccttcaa agtttttcaa ggtctccaaa atatttgcct
     4261 ggtcggtctt ttgcttgaga gtcacccaaa cggactcact atgagcattt aaagctggaa
     4321 ttcgtacggt aaaggctgaa accttaagct ccggaagact taaaatcttg cgggtttctt
     4381 tcatgatttt cacttcttca ctacaaaaac cttcctcatt aaaggaacca atttgcggaa
     4441 tacaattgaa aagaatggga tgaggaaaag tggtcggctt tggctttgga tttttataat
     4501 cctctgtttg cgccaggagt tcttcgtagc cagcaagacc agctccactc accgcctgat
     4561 aggaactcac acgcacttct tctaatccat acttatcagc caggggcttt aaggccacca
     4621 ctaattgaat ggtcgagcaa tttggattgg caatcacttg aggttttgaa tgtgaattca
     4681 ccaagtgacc attcacttct ggaacaatca gatgaacctt cggatccatg cggaaagccg
     4741 ctgaattatc gatggcgaag gctcctgctt tggccgcttg cggagcccac tctttgctga
     4801 tgtcgtcgcc agaggagaaa aacaccagat ccaaaccttc aaagcaatct ggtgctaaag
     4861 tctgaacaga ccaatcttga ccttgtacat ggattttttt acccacagag gcttcgctgg
     4921 caaaagggcg aagctccatc aaggggaact tacgctcttc aaggatattc ataaaagttt
     4981 ggcccaccat tccagtggcc ccaacaacgc cgaccttaaa gttttttatc atgtttagga
     5041 cttcccgttt cttcatcttc ttcgacaaga tcatcttcat ttacttgagc aggtaaatag
     5101 acacttggac ggatcttacg aacgttttta cccagtttaa aaactccaaa gaccgcaccc
     5161 aaaacagcgt atccaaagaa gagcacgaag agcataacct cagggtaaag ggctacgaca
     5221 accaagaccc ccactcccaa gagtaaataa tggaagggca tacgttcttt aagatcaaga
     5281 tctttgaaac tacgataacg gaaattactc accataacga aggccaaaag agctgtcatt
     5341 accaagaggc caatattacc actcgcttca agttctaaat cttgaaaggc taaaatagaa
     5401 gaggcaacaa taccggctgc catcggaatg ggaagacctt gaaaatagtt tttctccaca
     5461 atgttggctt ggacattaaa tcgagccaaa cggagtgcgc cgcaagtgac aaacaaaaag
     5521 catgcaaccc aacccaaacg cccaaaggga tttaaggccc attggtaaag cataatacct
     5581 ggtgccacac cgaagctgac taaatcgcag agagaatcat actcagcgcc aaatttactg
     5641 gtggaccgag tcaaacgtgc caaacggcca tcgagaaggt caaaaactgc cgcagccaca
     5701 atggcatagg ccccatatag aaaattagat tttaaggctt gaatgatggc aaaaaagccg
     5761 caaaacaaat ttccagtcgt catcaagttt ggcaatacat aaatgtaaat tgccagtttc
     5821 tttccagctg gttctacatt ttcagaagag gaatgattat cagccataaa atatgtcctt
     5881 tgaattggcc gc
//
LOCUS       DATBTE010000451         3100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1019669, whole genome shotgun sequence.
ACCESSION   DATBTE010000451 DATBTE010000000
VERSION     DATBTE010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019669"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /gene="rplD"
                     /locus_tag="VIG33_17190"
     CDS             <1..132
                     /gene="rplD"
                     /locus_tag="VIG33_17190"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEY8272632.1"
                     /translation="NLETFKYFPVEGLNVFDLLKYDAAIITKDSVAKIIDRCSLEKA"
     gene            132..410
                     /gene="rplW"
                     /locus_tag="VIG33_17195"
     CDS             132..410
                     /gene="rplW"
                     /locus_tag="VIG33_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEY8272633.1"
                     /translation="MKNVIKAPLITEKNTYHNAVGVYVFEVDMKSSKTEIRTAVEKNF
                     KVKVDSIRTSVCRGHSKNSKFGATKVPYWKKAYVKLTAGEKIALFEGV"
     gene            413..1231
                     /gene="rplB"
                     /locus_tag="VIG33_17200"
     CDS             413..1231
                     /gene="rplB"
                     /locus_tag="VIG33_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEY8272634.1"
                     /translation="MGIKVFSPRSDGRRGMTGFDFKEITKTTPEKSLTAPLNKKAARN
                     NHGQITMRHIGGGHKRKYRLIDFKREKLDVPAKVIAIEYDPNRTCRIALISYIDGAKA
                     YILAPIGLNVGDTVLSSDKADIKPGNSLSIGAIPVGTTIHNIELRPGKGGQVGRGAGA
                     GATLAGKGEKYCQIRLPSGELKQVLTVCRASVGQVGNTDNENISLGKAGRSRWRGIRP
                     GVRGMAMNPVDHPLGGGEGVGKGHHPVTPWGQPCKGYKTRHNKRTDSSIVKRRK"
     gene            1241..1537
                     /gene="rpsS"
                     /locus_tag="VIG33_17205"
     CDS             1241..1537
                     /gene="rpsS"
                     /locus_tag="VIG33_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEY8272635.1"
                     /translation="MARSTKKGPFIDDSLLKKIDNAIQKNDKKVIKTWSRRSTILPEA
                     LGLTFAVHNGRKFVPVYVTENMIGHKYGEFAPTRTFHGHAEKKAAPSAASAAKK"
     gene            1546..1878
                     /gene="rplV"
                     /locus_tag="VIG33_17210"
     CDS             1546..1878
                     /gene="rplV"
                     /locus_tag="VIG33_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEY8272636.1"
                     /translation="MEVKASLNYARVGAQKARLVADVVRGKDVNEAVKTLTFLNKKSA
                     ATIKKLIESAVANAEYKKVMDVDRLYIKSIWVDQGPVLKRFRPRAQGRAFGVRKKTSH
                     INVVLEEK"
     gene            1881..2546
                     /gene="rpsC"
                     /locus_tag="VIG33_17215"
     CDS             1881..2546
                     /gene="rpsC"
                     /locus_tag="VIG33_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEY8272637.1"
                     /translation="MGQKVNPIGLRVGVIRTWDSRWYAKGQQYYENLHEDIRLRKYLK
                     AKLKHAGVAKIEMERAAKKIKVIISTARPGVVIGKKGTGIDSLKADVQKLTPNEVFLS
                     IQEVRKPDLDAQLVAESIALQLEKRISWRRALKKAIAAAIKGGVRGIKVRVSGRLDGA
                     EIARSEWYNEKSVPLHTLRADIDYGTAEALTSYGIIGLKVWIYKGDILSAREVEEAGR
                     VKS"
     gene            2533..2940
                     /gene="rplP"
                     /locus_tag="VIG33_17220"
     CDS             2533..2940
                     /gene="rplP"
                     /locus_tag="VIG33_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEY8272638.1"
                     /translation="MLSPKRVKWRKQFVGRATGYAVRGANLDFGDYGLQATEEHRMTA
                     RQLEAARIAISRSVKRGGKIWCRIFPNIPVTKKAAETRMGSGKGNPEFWVARILPGKV
                     LFEMNGVTREQAKEAFERAAHKLPFKTRFLARE"
     gene            2946..>3100
                     /gene="rpmC"
                     /locus_tag="VIG33_17225"
     CDS             2946..>3100
                     /gene="rpmC"
                     /locus_tag="VIG33_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEY8272639.1"
                     /translation="MKFTEIKDLSVVELKKKRAGLSEELFQAQIKNTIGQLSSPIEIR
                     NLRRDIA"
BASE COUNT          942 a          528 c          758 g          872 t
ORIGIN      
        1 aaccttgaga ctttcaaata cttccctgtt gaaggtttaa atgtatttga tcttcttaaa
       61 tatgatgctg cgattattac aaaagattct gtagcgaaga ttatcgacag atgctcactg
      121 gagaaggcgt aatgaaaaat gtaattaaag cccctctcat tactgagaaa aatacttatc
      181 ataatgcagt tggtgtttat gtttttgaag tggacatgaa atcttcaaaa accgaaattc
      241 gcacagcggt tgagaaaaat tttaaagtca aagttgacag cattagaacc agtgtctgca
      301 gaggccattc taagaatagc aaatttggcg caactaaggt tccttactgg aaaaaagctt
      361 atgtgaagct tactgccggt gagaaaatcg cactctttga aggagtttag agatggggat
      421 caaagtattt agtccgcgct ctgatggcag aagaggaatg actggttttg attttaagga
      481 gatcactaag acgactccag aaaaatcact cacagctcct ttgaataaaa aagctgctcg
      541 taataatcat ggtcaaatca ccatgagaca tattggtggc ggtcataaaa gaaaatatcg
      601 tttgatcgat ttcaaacgtg aaaagctcga tgttccagct aaagttatcg caattgagta
      661 tgatccaaat agaacttgcc gtattgcttt gatttcttat atagatggcg cgaaggcata
      721 tattctagct ccaatcgggc tgaatgtcgg ggataccgta ttatctagcg ataaggcaga
      781 tatcaagcct ggaaattctt tatcaattgg tgcaattcct gtaggtacaa caattcataa
      841 tattgaattg cgtcctggta aggggggcca agttggtcgc ggtgccggcg cgggggctac
      901 cttggctggt aaaggtgaaa aatattgtca aattcgattg ccttcaggtg agttgaagca
      961 ggttttgaca gtctgccgtg cttctgttgg tcaggttggt aatactgata atgaaaatat
     1021 ttctttgggt aaagctggac gttctcgatg gagaggcatc cgccctggtg ttagaggtat
     1081 ggcgatgaat ccagtagatc atccacttgg tggtggtgag ggtgttggta aaggccacca
     1141 tcctgtaact ccatggggtc aaccttgtaa gggatataaa actagacaca ataaacgtac
     1201 tgactcttca attgttaaga gacgcaagta ggggtaaaaa gtggcacgtt caacgaaaaa
     1261 agggccgttc attgacgatt ctttgttaaa aaagatcgac aatgcgatcc aaaaaaatga
     1321 taagaaagtt attaaaactt ggtcacgtcg ctctacgatt cttccagagg ctctgggttt
     1381 gacctttgcg gttcataacg gaagaaagtt tgtgcctgtc tacgtgacag agaacatgat
     1441 tggtcataag tatggtgagt ttgcgccaac tagaactttc catggtcacg cagagaagaa
     1501 agcagcccca tcagcagctt ctgcggctaa gaaatagagg aatttatgga agttaaagcg
     1561 agtctaaatt acgcaagagt cggcgcacaa aaagcaagat tagttgcaga tgttgttcgt
     1621 ggaaaagatg taaatgaggc tgtaaaaacc ctcactttct tgaacaagaa atcggcagct
     1681 acgatcaaaa aattgattga gtctgctgta gctaacgctg agtacaaaaa agttatggat
     1741 gtagatagac tttacatcaa atctatttgg gttgatcaag gaccagttct taaaaggttc
     1801 cgtcctcgtg ctcaagggag agctttcggt gttcgtaaga agactagcca cattaacgta
     1861 gttctcgagg agaaatagta gtgggacaaa aggttaatcc aattggactt agagtcggtg
     1921 ttatcagaac ttgggattct cgttggtatg ctaaaggcca acaatattac gagaatcttc
     1981 atgaagatat tcgtcttaga aaatatttaa aagccaaatt gaagcatgct ggtgttgcca
     2041 aaattgaaat ggaacgtgca gcaaagaaaa ttaaagtcat tatttctact gcgcgtccag
     2101 gtgtagttat tggaaaaaaa ggcactggga ttgattctct taaggctgat gttcaaaagc
     2161 taactccgaa tgaagtgttt ttgagtatcc aagaagttcg taagcctgac cttgacgctc
     2221 aattggtggc ggagagtatt gctcttcagc tagagaagcg tatctcctgg agaagagctt
     2281 tgaagaaagc tattgctgct gcaatcaaag gcggagtgcg aggtattaag gttcgtgttt
     2341 ctggtcgtct tgatggtgct gagattgctc gttccgagtg gtataatgaa aaaagtgtcc
     2401 ccttgcatac tttgcgagcg gatattgatt atggtaccgc tgaagctttg acaagctacg
     2461 gtatcattgg tcttaaagta tggatatata agggcgatat tttgagcgct cgtgaagttg
     2521 aggaggctgg ccgtgttaag tcctaaaaga gttaaatggc gtaagcaatt cgtaggacgc
     2581 gctactggtt atgcagtacg tggtgctaat cttgattttg gtgattatgg tcttcaggca
     2641 actgaggagc atcgcatgac tgcgcgtcag cttgaggctg ctcgtattgc aatttcaaga
     2701 agcgttaagc gtggtggaaa aatttggtgt cgcattttcc ctaatatccc ggttacaaaa
     2761 aaagctgctg agactcgtat gggtagcggt aaaggtaatc ctgaattttg ggttgcgcgt
     2821 atccttcctg gtaaagttct ttttgaaatg aatggtgtaa ctcgcgaaca agctaaagaa
     2881 gcatttgagc gtgccgcgca taaacttcca tttaaaacca gattcttggc tcgggagtaa
     2941 tgatcatgaa atttactgaa attaaagatc tatccgttgt ggaacttaaa aagaaaagag
     3001 cgggcttgag tgaagaactc tttcaagcac aaattaaaaa tacaattggt cagctttcta
     3061 gtccaattga aattagaaac cttcgccgtg atattgctaa
//
LOCUS       DATBTE010000452         1910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_815325, whole genome shotgun sequence.
ACCESSION   DATBTE010000452 DATBTE010000000
VERSION     DATBTE010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815325"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="VIG33_17230"
     CDS             <1..911
                     /locus_tag="VIG33_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272640.1"
                     /translation="EYFIICLPYAMAGVFVMGVFLRAIIYYTVRRHEWFAREFEKRVN
                     RFMETEVPGKVEKVSFYVLSKKVLERTYYEVFEIRDRMKRRKPDAMMSWSDRLFLVKQ
                     GCAWLVKDILKQVKFLKWTEETPKLLHITKSTFNHNPCFNRIFGVFPMVGVNDVISIL
                     PGLFVVAGILGTFIGISGGLNDLGGMNLQDLENTKNIMDKFLHEISFAMKSSIVGIVF
                     SLLSHVANTVMSPERAYVSMIDRFEGAMDLLWYRSDNNDYPSDGKPFDEHKDAVDALA
                     EDALNKEISKGLRTREMLEVRKPKAS"
     gene            940..>1910
                     /locus_tag="VIG33_17235"
     CDS             940..>1910
                     /locus_tag="VIG33_17235"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY8272641.1"
                     /translation="MLNTVCHYTVTVHRKDQTMIKIVNEDSFTIGRSLDCTIPLPEDS
                     ISRVHLIVHRRQEQIWIEDKGSSNGTFVNNIRIAQNSLVNIAPSDLIRIGKSDYVVCI
                     SLEIEERGDAAVKDINNTDATDVLASPQSTKNQQKSTLQSIFTFKEKKPDLKEEIKQE
                     VKYEAPAHNNNPPQYEAEKILHEAHKKAAQIIYNGELQAEKRSQVIYAEARERQAQAE
                     SFYQKKIQEAHKEADKILLDFEQQGKGLLEQARQFAQEIRNEVDAFSRTLREKAWREA
                     EEVGIEARKEVEVFKQEAYEKARRDAETEAAELIVKGHEECKNLLKLA"
BASE COUNT          581 a          359 c          448 g          522 t
ORIGIN      
        1 cagaatattt tattatatgc ctgccctatg caatggccgg tgtgttcgta atgggagtct
       61 ttcttcgcgc cattatctat tacacggttc gccgccatga gtggtttgca cgggaatttg
      121 aaaaacgagt caaccgtttt atggaaactg aggttcctgg aaaagttgag aaggtctcct
      181 tctatgttct ttcaaaaaaa gttttagaga gaacttacta tgaggttttt gaaattcgtg
      241 atcgtatgaa aagaagaaag ccggacgcta tgatgagttg gagtgatcgg ctctttttgg
      301 ttaagcaagg ctgtgcgtgg ctggtaaaag atattttgaa acaagtgaag ttcttgaaat
      361 ggactgagga aactccgaag cttttacata ttacaaaatc gacgtttaat cacaatccct
      421 gctttaatcg catttttggc gtgtttccga tggtcggagt gaacgatgtc atttcgattc
      481 ttcccggact gtttgttgtt gctggtattt tgggtacttt tattggaatc tccggcggtt
      541 tgaacgacct tggcggaatg aatcttcaag acttggaaaa tacaaaaaat attatggata
      601 aatttcttca tgaaatctct ttcgccatga agtcatcaat tgtgggtatt gtgttctcac
      661 tgctgagtca tgttgccaat actgttatgt ctccagagag ggcctacgta tcaatgattg
      721 accgttttga aggtgctatg gatcttctgt ggtaccgcag tgacaacaat gactatccaa
      781 gtgatggaaa accttttgac gagcataaag acgcggttga tgccctcgca gaagatgctc
      841 taaacaagga gattagtaaa ggcttacgta cccgcgaaat gctcgaggta cgtaagccaa
      901 aagcttctta gatcgttaat attttatgga aactaaaaga tgttgaacac tgtttgccat
      961 tatacagtta cggtccatcg aaaggaccaa acaatgatca aaatcgtgaa tgaggattca
     1021 ttcacgattg gtcgttctct ggactgcact atcccgctgc cggaagatag tatcagtcgt
     1081 gttcatctta ttgttcatcg acgtcaagag caaatttgga ttgaggacaa aggctcttcg
     1141 aatggaactt ttgtaaacaa tattcgcatt gcgcaaaatt ccttggtcaa tattgccccc
     1201 agcgacctta ttcgcatagg aaaatcggat tatgtagttt gcatcagtct ggaaattgaa
     1261 gaacggggcg atgccgccgt gaaagatatt aacaatactg atgccacgga tgtgttagcc
     1321 agtccccagt cgacaaaaaa tcagcagaaa tccacgctgc aatccatctt tactttcaaa
     1381 gaaaaaaagc cagacctaaa ggaagagata aaacaggaag taaaatacga ggctcccgca
     1441 cacaacaaca atcctccaca atatgaagct gaaaaaatcc ttcatgaagc tcataagaaa
     1501 gcggcgcaaa ttatctataa tggagagttg caggctgaga agagatctca agttatctat
     1561 gctgaggcgc gtgaacgtca ggctcaagct gagagttttt atcaaaagaa aatccaagag
     1621 gctcataaag aagctgataa aatactttta gattttgagc agcaaggcaa aggtcttctt
     1681 gagcaagcaa gacaattcgc ccaagaaatt cgtaatgaag tggatgcgtt ttctcgtact
     1741 ctccgggaga aagcttggcg agaagccgaa gaagtgggca ttgaagcgcg caaagaagtt
     1801 gaagttttta agcaggaagc ttacgaaaag gctcgccgtg atgccgagac tgaagccgca
     1861 gagttgattg tcaaggggca cgaagaatgt aaaaatcttt tgaagctcgc
//
LOCUS       DATBTE010000453         2525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_199663, whole genome shotgun sequence.
ACCESSION   DATBTE010000453 DATBTE010000000
VERSION     DATBTE010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199663"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(688..1083)
                     /locus_tag="VIG33_17240"
     CDS             complement(688..1083)
                     /locus_tag="VIG33_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272642.1"
                     /translation="MRRFVTLFVSLTTFYSTLALAEGVDLVSCDVYLKKQDVHSTVTL
                     ENSHPQFVKVDGAKTLELSYGEDSIRLKLTRPPTKAELKAFKEKMGPNEVGLGPINIA
                     SAGTSFGGPLVGATLNSEYVYVNCNMETK"
     gene            1229..2116
                     /locus_tag="VIG33_17245"
     CDS             1229..2116
                     /locus_tag="VIG33_17245"
                     /inference="COORDINATES: protein motif:HMM:NF016847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8272643.1"
                     /translation="MPSPLLEELDQVLKQIIQRVTRYLEKQKIIIRDEEDHLQIHLDD
                     QDAFARLQASSSTYRFLTGPNKGKKALVLKTVDQDHSSYQGLVVKSSGFSLHAGVSCK
                     ADERKKLERICRYIARPAISEERLSVNQNGQVVYQLKKAYDDGTTHVVMEPLELLEKL
                     SAIIPRPRVHLTRFHGILAPHDKHRKQVVPKAEQAVSVAQSEIAVLTLQQQLLEKKKK
                     QQRMSWARLLKRVFNIDVETCGACGGKMKIIAAVEDPQVIKKILDHLGLPSTPPLAKP
                     ARGPPLSEWDDYHQTFFEE"
BASE COUNT          743 a          567 c          559 g          656 t
ORIGIN      
        1 agcagaggca ttgcgttcca tacgaataag ttttttctca gttcttggat gtaatttgat
       61 aaatcttggc gacatcttgc gatcttaggt cgacttttat tttacttcaa ctatgtcctt
      121 ttaattatgc aattatatat acaaattccc caatgggatt agctttgttc acgatcctcg
      181 gcgcgctggc acaaatggaa cgcgaaatta tcagaaatcg tgtcatcgct gggctctctt
      241 ttcgcgagtg cgcccgagtg gctggttgtt cgcacggaag tataagcgcc cagaaaaaga
      301 gtgggttgcg aagaaaaaac tggctcgtga aaaagaggaa tctcaaaagg ccatcagctt
      361 agcagttcaa gaaaatggat cacctgcacc gttgccagac gtaccatcac ctgaagtgaa
      421 agctcccaat gaacagttaa tttctccatg atcacgaccg ccgtcgattc tcaattagag
      481 ggtcgacggc cctttttttt gccttgtgtg ttcaggaagg cgaacctttc gatccaattc
      541 ccaacatttt tgaaattttc aagtgcgcct gtgctttgtt gaccgagatg ttaccgaagt
      601 cgaatttcgg ccccagtcta tattgctcgg tctggaattc gggagttaaa accaagtggt
      661 ttgaagctct tcaaaaattc gactaaatta ttttgtttcc atattacaat ttacataaac
      721 atattccgaa tttaaggtag caccaactaa tggaccacca aatgaagtgc ctgcgctcgc
      781 gatgttaatt ggcccaagtc ctacttcatt aggacccatc ttctctttaa atgctttgag
      841 ttctgccttc gtcggtggtc tggtcagctt taaacgaata gagtcctctc cataagaaag
      901 ctcaagcgtc tttgctccat caactttaac aaattgaggg tgactatttt ctaaagtgac
      961 agttgaatgt acatcttgct tcttcaaata tacgtcacaa cttactaaat ctaccccctc
     1021 tgcaagtgct agggttgaat aaaaagtagt caaagaaaca aaaagtgtta caaaacgtcg
     1081 cataaatact cctattttgg ttaatgggtt tgagctaaat acgatgcaaa attaattcca
     1141 atttgcgtga ccacgaattg aatcatagga aatcgtaccg aaagaggcac ttgacttaag
     1201 ttggggccac taaaggcagt tccactcggt gccctcaccc ttacttgagg agctggatca
     1261 agttctaaag caaatcatcc aaagagtaac caggtatctg gagaagcaaa aaatcatcat
     1321 ccgggatgag gaagaccatt tacagatcca ccttgatgac caagatgcgt tcgcaagact
     1381 gcaagccagc tcgagcactt accgattttt gacgggtccg aataagggca aaaaggcctt
     1441 ggtgctaaaa accgtggacc aagaccacag ctcctatcag gggctggtgg ttaaatccag
     1501 tggcttttcc ctgcacgccg gagtcagttg taaagccgac gagcggaaga agcttgagcg
     1561 aatctgtcgg tatattgcaa ggcccgcgat cagtgaagag cggctgtcgg tgaatcaaaa
     1621 cggccaggtg gtgtaccaac tcaaaaaagc ctacgatgac gggacaaccc atgttgtgat
     1681 ggagccgctt gagcttctgg aaaagctctc agcgattatc ccgaggccac gagtgcacct
     1741 gactcgattc cacgggattt tagcgcccca tgataagcac cgaaagcagg ttgtgccaaa
     1801 ggctgagcag gcggtgtcgg tggcgcaatc tgagatcgcg gtactcactc tgcagcagca
     1861 gcttcttgag aaaaagaaaa agcaacaaag aatgagttgg gcacggcttc taaaaagagt
     1921 cttcaacatc gacgttgaaa cctgtggagc ctgcggtggg aaaatgaaaa tcatcgctgc
     1981 cgtagaagac ccgcaggtga tcaagaaaat cctggatcat ctggggttgc cttcaacgcc
     2041 acctttagcc aagcccgcac gaggacctcc actctcagag tgggatgact atcaccaaac
     2101 attctttgaa gagtaagtct ccaatatctg gtcagaaaaa aaagaccttg aggggatcgg
     2161 tgtctctaaa acccagtcaa ctccatgaga atcctcaaaa agtatccacc aagggtaaaa
     2221 agctcttgct cgacgccgga aaaacccatc aaataatcaa agcagcgagc agcagggccg
     2281 gataagaaca aaaaaccata ttcaggaaaa ctctaaaatg gtgtttatac ttcctatcct
     2341 ctcataatat attattgcta tttaattttc ttcgaataaa tcgcagttaa ttgaaattcc
     2401 atcccacgat gttgttgggc tttttaacgt aacttgcttt tcactttgag cgacatagcc
     2461 gttagattta attgaaacaa agcttcctga tagattatcg ttgatggtgc taaatgatgc
     2521 ttcga
//
LOCUS       DATBTE010000454        17404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2317870, whole genome shotgun sequence.
ACCESSION   DATBTE010000454 DATBTE010000000
VERSION     DATBTE010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 17404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17404
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2317870"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VIG33_17250"
     CDS             <1..663
                     /locus_tag="VIG33_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272644.1"
                     /translation="AHDFDNALALYDSITQKYDGSPKDFDFDQIYKRKIAYFARIQRN
                     PKAAIANLKADAANSKLPKNVKKNLKDWIHYFESWSEEGAKDPAKLSNENFLAYAKAE
                     TDKNTSGRKISIADPAIVNLLRVSGLLYERVFKTPNVTSTPEMLYLLAKCERDLSPIQ
                     KYSLADIYLRECVLEFPKSSIAKKCFKDYEISLKQKVTFDSDYIKGNIDFLRRHMENS
                     AN"
     gene            complement(730..1557)
                     /locus_tag="VIG33_17255"
     CDS             complement(730..1557)
                     /locus_tag="VIG33_17255"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY8272645.1"
                     /translation="MEKRKLGNSGLEVSAIGFGCMNIVWAYGPGVPREQARNILQAAF
                     DDGVTFFDTAEIYGPFTSEVYVGEALKSVRDKVVIATKFGFDIADDGGIRGLNSKPQY
                     IRDVVEASLKCLQTDHIDLLYQHRIDPQVPIEEVAGAVKNLVKEGKVLHFGLSEAGPK
                     TIRRAHAVLPLAAIQNEYSLWSREPERAVLGICEELGIGFVPWSPLGMVYSTGKVDEN
                     TKFSLQGDLRTGFPRFTPEAIKANHAIVEILESIAQQKNATTAQVALAWPLAQPRHS"
     gene            complement(2010..2669)
                     /locus_tag="VIG33_17260"
     CDS             complement(2010..2669)
                     /locus_tag="VIG33_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272646.1"
                     /translation="MRTKNFLLVILIMTGLKSFADTVPHGIDLASPCRYLDKDKLPVI
                     AEVCFRHSRNHFMVVGCGGKKCLTTSAIKNTKERCLKRKDGMPNLNPTGMTFMSPVST
                     NASISQKNDSTQSNCEDIGANYFEHGYDLFKHTADYQKFFYKNEYTISPGAMSLKTAK
                     LFLKEMLKSPINQKFDPRKSGIDYIVFETETDDLTEKNIYFTRENKLVSAKDIGIAEC
                     E"
     gene            complement(2587..3489)
                     /locus_tag="VIG33_17265"
     CDS             complement(2587..3489)
                     /locus_tag="VIG33_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272647.1"
                     /translation="MLNQKNKSRVSSLQNKQAGLQVLVEPLSFQQLLGLLSVAVVLIY
                     AFTWGYIYQLGIPESPDLAHATYFFVSIFVGVLFKGSLHYLISYNSFYQVIFWFLVGA
                     LIAWGVFARWLPITAKRRIQNFIKQRQVGGIVLKILMFFVLVWPPIATFLMNSIFMPG
                     MVYSYILDDWILLDKKFIVLISGVVAAFIIPIVFPLIAKNIKRNIGILVWLFLIFYSA
                     LFCFYLSTESIEFMNFPIAVTDREKPIRYPHLWVSDKGRYIAACNESTWRLIGVSYEN
                     KEFFISDPHYDGFEIVCRYRTPWN"
     gene            3885..4658
                     /locus_tag="VIG33_17270"
     CDS             3885..4658
                     /locus_tag="VIG33_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEY8272648.1"
                     /translation="MKLISWNVNGLRSVHKKNFREWFEKEKADVVCLQEIKITDEVLR
                     KDEGFYHPARYHSSWAFAEKAGYSGLALYSRKEPDDVRVGLGIAKFDREGRWLEADFG
                     SITVINSYFPNSQREHTRLPFKLEFCAAAEKRLQALRKQGREVVICGDFNIAHKEIDL
                     RNPKTNIKNAGFLPEERAWMTKFLDKLEWVDSFRKFEPGPDHYTWWSYRPGVREKNIG
                     WRLDYFLVNKEASDRLKAAAHCPDVMGSDHCPVRLTLKK"
     gene            4662..5270
                     /locus_tag="VIG33_17275"
     CDS             4662..5270
                     /locus_tag="VIG33_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEY8272649.1"
                     /translation="MLKKYFPEREFKALLFDFDGTVADTMGAHLHAWNRGLANYNLSL
                     SKEQHLAWAGRPTRMIVQLLNEMHKIEISIEEFLKQKEIHFFSSLHEVKEIRSVVEIV
                     KHYHGTLPMAVVTGSRRAAVEKTLAHLGLTGYFNQLVCAEDYTHGKPAPECFLLGALR
                     LDIEPKDCLAFEDAPLGIEAAKKAGMSCLHVNENHEPTVVKY"
     gene            complement(5290..6183)
                     /locus_tag="VIG33_17280"
     CDS             complement(5290..6183)
                     /locus_tag="VIG33_17280"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEY8272650.1"
                     /translation="MKPKNVAASVAAKLLKHSKDSGRNYNEILVYYGIERFLARLSKS
                     KFNSQFVLKGAMALHVLNADLARSTRDIDFLAFESNDPDKMKKIISLICEINLEDGVL
                     FDLKSLETEIIKEDAEYQGVRVSFLGKLEKAEIPLQVDIAVGDTMTPKPEELAFPVIL
                     GNGFRLKVYPKETVIAEKLQAMVLLDMLNSRMKDFFDIWFLATNFSFDGDLLQKAIEA
                     TFKNRETDLVALPTALTPEFYLDKNKTLQWSAFRKRIANSAPESLEEVCKIVFNFAGP
                     ILEASAARDKFAMKWNAPEWR"
     gene            complement(6180..6794)
                     /locus_tag="VIG33_17285"
     CDS             complement(6180..6794)
                     /locus_tag="VIG33_17285"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEY8272651.1"
                     /translation="MKDKKSIAEEKALAYFKKAGGVIRTSQAMQQGIRPEILYKLRDK
                     GILETMGRGVYKLSDKTPGVDPQIIRLGLKHKNAVLCLISALFIHKLTTQIPRRIYVA
                     LPKGSHPPEKVKNKVEFFTISPEQYELGIEQHEIDGWKFKVYDPEKTIVDCFKFRNKM
                     GIDLAIESLKEWKKKRPKNLKKLLAYAKACRMEKVMAPYLEAIA"
     gene            complement(6895..7647)
                     /locus_tag="VIG33_17290"
     CDS             complement(6895..7647)
                     /locus_tag="VIG33_17290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY8272652.1"
                     /translation="MTKEWPDRAGSKQEEKIFLEGRHTRGFELKRAFRIFLECIKGFR
                     ALHFVGPCITVFGSARFKEGHPYYVLAKRFGAEIAKRGFTVITGGGPGLMEAANLGAW
                     EVQGRSIGCNIVLPREQKPNKYLDRWVEFKYFFVRKLMLAKYSYAFIVMPGGFGTLDE
                     FFEVATLMQTGKMQGFPVILVGKDYWEGLLAFLKDPVLVAGNISSSDLESIKLTDDPV
                     EALDLITEAVVKKFGLKYAKKKKPWWFMGERN"
     gene            7819..8823
                     /locus_tag="VIG33_17295"
     CDS             7819..8823
                     /locus_tag="VIG33_17295"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEY8272653.1"
                     /translation="MEARKTILGAMTMLFTVSAFADDCDSIRKAINDLPPAGGTVLID
                     KGIYNCGAPIIINRSNVNLKGAGQNKVTLRLIDHVHAPLLIIGEPKTVMNEHGNYVAE
                     HRVENIRVSGLTLDGNRANHDPSKECGETICEGDPSAVRNNGITIRGASKVLVENVTT
                     HSMISGGLVTEKYCDQLIIRNFNSYDNYFDGFAGYETEHSVLSGLYLHNNRGAGISID
                     INFNNNIIRDSKLHDNGDVGIFARNLSGNQFKRLSIQRSGNHGVFLASAEAPNSCAHD
                     NQFEAVLINGSKRSGFRLNNNCQGNRITGASNLCGNAEGGFSEDRQGTLLVADKVSCR
                     "
     gene            8887..10263
                     /locus_tag="VIG33_17300"
     CDS             8887..10263
                     /locus_tag="VIG33_17300"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEY8272654.1"
                     /translation="MFRIKACLFSYILIALTPFAANSQQSSLEQWMKTEGNIALTRMN
                     QNISPTGAARGVVIASPSRQDPDYYFHWTRDAALVMETFYLPQTFADYTEFSRQTQLV
                     STLTGLGEPRFNIDGSAYTGPWARPQNDGPALRVLTILRSLRRPQISSATAQATAQTT
                     VQAIVQTDLQYILNYFRTPSFCLWEEVKGDHFYTRLVQMAALKESLEHFDSNWNIPRQ
                     TLQTAMTSLQNELARHWRDDKKYFSATLNRIEGAENYKFTDLDTAVILAVLHANLETD
                     EFSVKDDRILATASQLETAFAATYEINAPTYASPYPVAPAIGRYTDDIYFGGNPWYIT
                     TAAFAELHYRLSLQLMKNNSYVVTELNLPFLKKALALRGSDSDVAELQSGLNLKTRPQ
                     LVKLLQEGLLARGDLFMARLRLHTPVDGSMAEQFARKDGQPVSARDLTWSYASFLSAL
                     KSRKALFE"
     gene            10333..12894
                     /locus_tag="VIG33_17305"
     CDS             10333..12894
                     /locus_tag="VIG33_17305"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEY8272655.1"
                     /translation="MGLKNLIVLVTAAFFTLPATTALAGLYRVHQNQLWIDNEPQPQL
                     FGAEVQYFRMRGGYERNIPRDRVMALWAQALDRLVEAKMNVISFYIPWDFHEYAPGKF
                     DFDGTADEDHDGNPDYPSRDLQTFFQMIEAHGIHKIMIRPGPYINAEWGFLGFGAVPL
                     WFHEKYPQSHMQNAQGLSTKLYDYHNPDLLRHTRLWFEELYRQVLKNKIGPGKPISFL
                     QIDNETNFMWQSLYNHDYNPAALRRYRDFLKSRYQDLATLNAHHHRAWSRWEEVMAPT
                     SPGLNIAEDQDWYRFADESIYLYLQKIRALWENLGVREPTVMFTLADSFNATQNGLLP
                     NYQLHNTPGVTGVMTVNLYPKTYETPEHPLLNLPFKADHDVKAAEAASELYWGNKERW
                     VLGPEIQGGWWRGIDVTSEARRQTYLTVLGHGLKAFFVYYFNEGDNWQSDWAKQQIRP
                     LFEELRKEPPFVNVSEDQLSDEFWKTLQDRVDHRILVGFDVRGIMHEDQKIASQLYFD
                     APLDREAQPRDHYFELKELGEKLVTPYGRWLGRAVETTDPVCLIKDVNDHVPSAVANI
                     DSIQMNSDWSGGLIAYALRTGINVRVVHWGLNATVNALSELQDCRLILRQDAGGTSQA
                     LAETLQKLITQGHTVVNFLDDSLAKQIGIAVTKTVQPSTGPVLLNFEGQSFGAQSSPL
                     FHYNTMEDARCSAAITHETAAAAYRCAIGKGMFLQIGALFYDVYNSNQYALLSDLSNR
                     ELVLKSLLSSLKITPAVHVDADKVVAFARQVKGEAPLWVTVKSALQQATLTHIQVRDL
                     QPNQLYSVRNLLSGELQRLRGATIQQTGFAAQLAAFGSTVFWLEPISMTAHREKK"
     gene            12894..14222
                     /locus_tag="VIG33_17310"
     CDS             12894..14222
                     /locus_tag="VIG33_17310"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HEY8272656.1"
                     /translation="MMRFKGTWIFLGLLSIAACSTPSVREPSSAPLPSPIKKTMDMAR
                     LRSEAFTRCQTPFSRPVVEAVPILDPPHGGADAEGMVYQEELAVRTTNLLDREGFYFS
                     PRPRAAVNTLPMEIFVDGDNTGTYERKTWEDANPDYSSNQIQPPILQSNIPSAGRVLT
                     ELIIGHRSPRGDYPQLFDLRSSAYFRFAGYPPQVTGASLRLAAHKIFGLGGKGETATK
                     EDFPIVRAIYTGIKDSKTANALVLVESELFCGALNMDMLEGDNPEVVIDSYWYTRSDF
                     NWKRDPHTGLVAYSSMLWKTEKQTPERVSDEAHDSDILTVKTENGVVKKYPLDPPKTG
                     LRVRHLTPTQQPVEWTLANEDRDPSHFADFQPALGNTNYNFRASYRVSLLETNVRTGV
                     SLYEQAADGEYGDNIVAVSTLRQNIKKATKVDQFIRFKYKTTAFFVGDRF"
     gene            complement(14604..14987)
                     /locus_tag="VIG33_17315"
     CDS             complement(14604..14987)
                     /locus_tag="VIG33_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY8272657.1"
                     /translation="MNQKMVDIDQILEDDKLMLEGIFGNKTAEKVLLHVFHYGESHAS
                     GIAKDFKMSLNPIIQQLNRFEQAGVLVSKEVGRSRVYSFNPKSPLSKPVRALIEIAYE
                     AISLSERQAIFQTHRRPRRKGKPIL"
     gene            15179..15901
                     /locus_tag="VIG33_17320"
     CDS             15179..15901
                     /locus_tag="VIG33_17320"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEY8272658.1"
                     /translation="MTNNIAIIFSVLLFSLGLYAQEKSPWTHESEASIVSVGGNTTSE
                     SYSAKQKTSYKFDLNILTASARYLQTKASGTETAKQWDASLRYERELSENWAVFIQHG
                     AESDPYAGYVQRDNSDIGGKYYFIKTDLETLLSEVGLRATKTILSPTNEVTYTTSGRL
                     YVEYSKKINQSVSGKLWAEYLQNFKDSDAYLVNYEPSLSVMMNQIFSVKLAYLVKYHN
                     KTITPVEKKEDTTFTTALVAKF"
     gene            16149..16307
                     /locus_tag="VIG33_17325"
     CDS             16149..16307
                     /locus_tag="VIG33_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272659.1"
                     /translation="MICQCERNERCHVDLMLLIAKKNLGARIGELAYAYSEFCELRQW
                     QYPKSHGD"
     gene            16433..16834
                     /locus_tag="VIG33_17330"
     CDS             16433..16834
                     /locus_tag="VIG33_17330"
                     /inference="COORDINATES: protein motif:HMM:NF016211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HEY8272660.1"
                     /translation="MIGRIIQLSRKTLDVFFVVAGVLFLLIGIVGIVLPLLPTTPFLL
                     LSAFCFSKGSKKFHHWLLNHSILGSPIRDWERKKIIRKKSKILATVMMAGSSVFVLSK
                     DTIPLIGKVSYSLVLISVLIFIWTRRSTEEL"
     gene            complement(16836..>17404)
                     /locus_tag="VIG33_17335"
     CDS             complement(16836..>17404)
                     /locus_tag="VIG33_17335"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272661.1"
                     /translation="AFHWILYIFGFILVITAIKFLRETDEKIDAQENWSVRLLKRLIP
                     TTEKSNGSHFFIVEDGKRKATPIFLALVVIEVTDLVFAVDSIPAVFAVTQDAFVAFAS
                     NILAILGLRAIYFVLADWVAKLRYLKPGLAAILGFIGIKMLVVDFFKIPSWISLLVIF
                     AILTTAGLSSWYVARIEERQKGKTDPFA"
BASE COUNT         4721 a         4189 c         3758 g         4736 t
ORIGIN      
        1 gcccatgatt tcgacaatgc tttggcactc tatgattcga tcacacaaaa atacgatgga
       61 agtccgaaag attttgattt tgaccaaatt tacaagcgca agatcgcgta ttttgcccga
      121 attcaaagaa atcctaaagc agcaattgct aatttaaaag ccgacgccgc gaattccaaa
      181 ttgcccaaaa atgtaaaaaa gaaccttaag gactggattc actattttga atcttggagc
      241 gaggaaggtg cgaaggatcc agcaaagctc agcaacgaaa attttctcgc ctacgcaaag
      301 gcagaaacag acaaaaacac cagtggccgt aagatttcca ttgcggatcc ggccatcgtc
      361 aatcttctcc gggtctctgg cctcctttat gagagggtat ttaaaactcc caatgtcact
      421 tccactccag aaatgcttta tctattagcc aaatgcgagc gagacttaag tcctattcaa
      481 aaatattccc tcgcagatat ctatctgagg gagtgcgttt tagagttccc gaaaagcagc
      541 atcgctaaga aatgctttaa agactatgaa atttccctga aacaaaaagt gacctttgat
      601 tcggattaca tcaaaggtaa tatcgatttt ttgcgccgac acatggaaaa cagcgccaac
      661 tgaatgttca cccttctcat attgaccttt ggtagtgggc tgctctttag gtcatttcgt
      721 cgctcatatt caagagtgcc tcggttgagc aagcggccaa gccagcgcga cctgcgccgt
      781 tgttgcgttc ttttgctggg cgatggattc aaggatctca acgatggcat gatttgcttt
      841 tattgcttca ggtgtaaagc gaggaaaacc cgtccgtagg tccccttgca aactgaattt
      901 tgtattctca tccaccttgc ccgtggaata aaccattccc agcgggctcc agggaacaaa
      961 tccgattcca agttcttcgc aaattcctaa aacggctcgt tctggctccc ttgaccaaag
     1021 cgagtattca ttttgaatgg cggcaagggg caaaaccgca tgcgcccgac gaatcgtttt
     1081 aggtcctgct tccgagagcc cgaaatgcag aactttgcct tccttcacaa gattcttcac
     1141 cgcccctgcg acctcttcaa tgggcacttg cggatcaatg cggtgttgat agagaagatc
     1201 aatgtgatca gtttgaaggc atttaagaga ggcctcaact acgtccctta tatattgagg
     1261 cttgctgtta aggccgcgaa ttcctccatc atcagcgata tcgaatccaa atttcgtcgc
     1321 gatgacgact ttatcacgaa ccgactttaa ggcttcccca acgtatactt cgctcgtaaa
     1381 tgggccgtag atttcagcgg tatcaaaaaa cgtgacacca tcatcgaaag ccgcctgaag
     1441 gatattgcgg gcttgttcgc gagggactcc ggggccatag gcccatacga tattcataca
     1501 accgaatccg atagcggata cttctaggcc tgagtttccg agcttgcgtt tttccatgct
     1561 gtgtctcctt tactgctttg agactggtgg gcttggtttg gggctgtgcc tatatttgga
     1621 atattattct agttcgcttg ttggaaaatt atcgtatgag ttcgtgacct tatggataag
     1681 gctaacaaat gacagaggtg ggtgaatgaa ataccaaaag cttagcttgg aaaatgcaca
     1741 tccgatgttc ggacgtcgcc gtcagctcct aatacaaaaa cacaatgcat caataaaact
     1801 cttagccatt cgatgaaata tatccgtttt aaaggcatat tttcagcatc gatgaccatc
     1861 acggccgagc taactcattg caatgccgac gccaaatttt ctcttccttt ccaattaaat
     1921 tctctcggct tgtaatgtgc aatttcaaaa taccatttaa gcacaagcat ttatggtgct
     1981 tcagttggga attgaataga acctctcaat cattcacatt ccgcaattcc aatatctttt
     2041 gctgagacta atttattttc ccgtgtaaaa tagatatttt tttctgtgag gtcatctgtt
     2101 tcagtttcaa atacaatata gtctatacca gattttcgtg gatcaaattt ctggtttatt
     2161 ggggatttta acatctcctt taaaaacaat ttagctgttt ttaaagacat agctccgggt
     2221 gaaatagtat attcattttt ataaaaaaac ttttgatagt cagcagtatg tttgaataga
     2281 tcgtacccat gctcaaaata attagcccca atatcttcgc aattactttg agtactatca
     2341 ttcttttggg atatagaagc attcgtactt actggactca tgaaagtcat gccggtagga
     2401 tttaggttgg gcatcccgtc ttttcttttt aggcaccttt cttttgtatt cttgattgcc
     2461 gacgtggtta agcacttctt tcctccacag ccaacgacca taaagtgatt tctactatgt
     2521 cgaaaacaca cctcagctat tacaggcaat ttatctttgt ctaaatacct acatggactt
     2581 gctaggtcaa ttccatgggg tacggtatct gcaaacgatt tcaaacccgt cataatgagg
     2641 atcactaata aaaaattctt tgttctcata tgaaacacca attaacctcc acgtactttc
     2701 attgcaagca gcaatatatc tccctttatc agaaacccaa agatgcggat acctaatagg
     2761 cttttcccta tctgtcactg cgattggaaa attcataaat tcaattgatt ctgtagaaag
     2821 ataaaaacaa aataatgcac tgtaaaaaat taaaaacagc cagacaagta tcccaatatt
     2881 tcgctttatg tttttagcaa ttaaaggaaa aacaatggga ataataaatg cagctacaac
     2941 accagaaatc aaaactataa attttttatc gagaagaatc cagtcatcta gtatatagct
     3001 ataaaccatc ccgggcataa aaatagaatt catcaaaaaa gttgcaattg ggggccacac
     3061 caaaacaaaa aacattaaaa tcttaagaac aatcccacct acctgccgtt gttttatgaa
     3121 attttgaatt cgtcttttcg cagttatcgg aagccatcta gcaaataccc cccacgcgat
     3181 caaggcaccg actaaaaacc aaaaaataac ctgataaaat gagttatagg aaatcaaata
     3241 atgtaatgac cctttaaaca acacaccaac aaatatagat acaaaaaagt aagtagcgtg
     3301 ggcaagatct gggctttcag gtattccgag ctgataaata tagccccatg taaaagcata
     3361 aattaaaact accgcgactg acaagagacc aagaagctgt tgaaaactta aaggctcaac
     3421 taatacctgc aagccagctt gtttattttg taaggaggaa actcggcttt tatttttttg
     3481 attaagcatt caactgttat tctatttttt tttgcattct tcaaacagta aatttatcct
     3541 tttgtcgctt ttagaagggc ttatgttgat atatcgcacc cctcctcttt tatttggaac
     3601 tcgatattta aattgcttta agttttggct taagatccat atattcaact cttttacggc
     3661 ataaattgaa ttctggctct accccaaaaa ctttcgttta aggatttgcc ctaactttac
     3721 tcagcttcga gcgatcactt ccgatatttg aaattacaac aatcattctg gcgatttcag
     3781 aaggacgttt ttgaatttga cctgtctgaa caccaccaat tcagaaaaac tttcgcgatc
     3841 tcctcccctt gaactcccgc ccctttcatg caaccctacc ctctatgaaa cttatctcct
     3901 ggaatgtaaa cggcctgcgc tctgtccaca aaaagaactt tcgcgagtgg ttcgagaaag
     3961 aaaaggcgga cgtggtctgc cttcaagaaa tcaagatcac cgatgaagtc ctgcgaaagg
     4021 acgagggctt ttatcaccct gcccgttatc attcttcgtg ggcctttgcg gagaaagccg
     4081 gatactcggg tttggcgctg tactctcgta aagagcccga tgacgttcgt gtcggcttag
     4141 gcattgcgaa atttgatcgc gaaggccgtt ggcttgaagc ggattttggc tctatcaccg
     4201 tgattaatag ctattttccg aatagccagc gggagcatac acgccttccg tttaaactgg
     4261 aattttgcgc cgccgctgag aaaagacttc aagcccttcg taagcaagga cgtgaagtcg
     4321 ttatctgtgg cgactttaat attgctcaca aggaaatcga cctgcgaaat cccaaaacta
     4381 atataaaaaa tgcaggcttc ctgcctgaag agcgcgcctg gatgacgaag ttcttggata
     4441 aactcgagtg ggtggatagt tttcgcaaat tcgagccagg tcccgatcat tatacttggt
     4501 ggagttaccg tcctggtgta agagagaaga atattggttg gcgattggat tatttcttgg
     4561 tgaacaagga agcctccgat cgcctgaaag ccgcggctca ttgcccggat gtgatgggct
     4621 ctgatcactg cccggtccgg ctgacattaa agaagtaacc catgcttaaa aaatattttc
     4681 ctgaacgtga atttaaagcc cttctttttg atttcgacgg caccgtcgct gataccatgg
     4741 gcgcccactt gcacgcttgg aaccgagggc tggcgaacta caatttgagc ttaagcaaag
     4801 agcaacacct ggcctgggcc ggccgtccca cgcgcatgat tgtgcagctc cttaatgaaa
     4861 tgcataaaat cgagatttcc atcgaagagt ttctcaaaca aaaagaaatc catttcttct
     4921 catcattgca tgaggtgaag gaaattcgtt cggtcgtgga gattgttaaa cattaccacg
     4981 gaacccttcc catggctgtc gttaccggca gccgtcgtgc cgccgtcgaa aagaccttgg
     5041 ctcatctcgg cctaacagga tattttaatc agcttgtttg cgctgaagac tatacccatg
     5101 gcaaaccagc gccggaatgc tttcttctag gagctttacg tctcgatatc gagccaaaag
     5161 attgtctcgc ttttgaggat gcgcccctcg ggattgaggc cgcaaaaaag gcaggaatga
     5221 gttgtctgca cgtcaacgag aaccatgagc cgacagtggt gaagtattaa ataaatacaa
     5281 ttcctgaatt catcgccact ccggtgcatt ccacttcata gcaaacttat ccctcgccgc
     5341 actcgcctct aagattggcc cagcaaaatt gaatacaatc ttacaaactt cctccaaaga
     5401 ttccggcgca gaatttgcga ttctttttct gaaagcgctc cactggaggg ttttattttt
     5461 atccagatag aactcagggg tcagagccgt tggcaatgct accagatctg tttccctatt
     5521 tttaaaagtg gcttcaatgg ctttttgcag aagatcgccg tcaaaggaaa agtttgtggc
     5581 caaaaaccaa atatcaaaaa agtctttcat cctgctgttc aacatatcta gaaggaccat
     5641 agcctgaagt ttttctgcaa tcactgtttc ctttggatag acctttaatc tgaacccatt
     5701 tccgagaatg acaggaaatg ccaattcctc aggctttggg gtcatcgtat cccctactgc
     5761 aatatcaacc tgcaagggaa tctccgcttt ttctagcttt ccgagaaaag agactcgtac
     5821 cccttggtat tcagcatcct ctttgataat ttcagtctcc aaagatttca gatcaaagag
     5881 aactccgtcc tctaggttaa tctcacaaat taaggaaata atttttttca ttttatctgg
     5941 atcgtttgac tcgaatgcca ggaaatcaat gtctcgtgta gatcgagcaa gatctgcatt
     6001 taacacatga agtgccatcg ccccctttag aacaaattgt gagttaaatt tagattttga
     6061 taaccttgct aggaaccgct cgattccgta atataccaag atctcgttgt agtttcgccc
     6121 ggaatcttta ctatgcttta aaagtttagc ggcaacggat gcggctacat ttttaggctt
     6181 catgctatag cctccagata gggagccatg actttctcca ttcggcaggc tttggcataa
     6241 gccaaaagct ttttcaaatt tttagggcgt ttcttcttcc actccttcag ggattcaatt
     6301 gccaaatcaa ttcccatctt atttctaaac ttgaagcaat caacaatggt cttttctgga
     6361 tcatagactt tgaacttcca tccatctatc tcatgttgct ctatgcctag ttcatactgc
     6421 tcaggagata ttgtgaagaa ctccacctta ttttttactt tttctggcgg gtgagaaccc
     6481 tttggcaaag cgacgtatat tcgtcttgga atttgagtgg tcagcttatg aataaagagg
     6541 gctgagatca gacaaagcac ggcattcttg tgcttgagac caagccttat gatctgtggg
     6601 tccacgcctg gagtcttatc agaaagtttg tatacgcccc gccccattgt ctcgagaata
     6661 cccttatccc gaagcttgta caaaatctca ggacgaatgc cttgctgcat agcctgactg
     6721 gtcctaatga ctccgcctgc ctttttaaag taggctaagg ccttctcttc tgcgatcgac
     6781 ttcttatctt tcatgtgtgt aaatatatgc tatacttgtt aatatgtata gcatatattt
     6841 atattcttaa tttgagacag gaccttatta aagtaatctc ccgacctcaa accttcagtt
     6901 ccgctctccc ataaaccacc aaggtttttt ctttttcgcg tatttcagtc cgaatttctt
     6961 tacgacggcc tcggtaatta aatcgagggc ttcgacggga tcgtctgtca gctttataga
     7021 ctccaaatcc gaggaagaaa tatttcccgc gacaagcact gggtctttta gaaatgccaa
     7081 taacccttcc caataatcct tgcccacaag aatgactgga aaaccttgca tctttccggt
     7141 ttgcatcaga gtcgcaactt cgaaaaattc gtcgagagtt ccgaaacctc caggcatgac
     7201 aatgaaagca taagagtatt ttgcaagcat cagcttgcgc acgaaaaaat atttgaactc
     7261 gacccagcga tcgagatatt tgttgggttt ttgttccctc ggtaaaacga tgttgcagcc
     7321 aatggatcgt ccttggacct cccaagctcc caagttcgcg gcttccatca aacccgggcc
     7381 tccccccgtg atcacggtga atcctcgctt tgcgatctcc gctccaaagc gcttggctaa
     7441 tacataatag ggatggcctt ctttaaatcg ggcggagccg aaaaccgtga tgcacggccc
     7501 gacgaagtga agcgcgcgaa accccttgat gcattcaaga aaaatacgaa aggctcgctt
     7561 gagctcgaag ccgcgagtgt gacgcccctc taaaaatatt ttttcttcct gtttcgaacc
     7621 tgctctgtca ggccattctt ttgtcatggg ttgcacctca ctcaatatct ataatctctg
     7681 ttcgaaatat ttcccattaa gaagatcaaa cactcgtcaa agagatatta ccaccggaac
     7741 ctcgccaccg gtaaggcttt tgcatttgta tcttcatcgg aatggacatc gatttaaaat
     7801 acgaaagaga ggtttctcat ggaagcaagg aagacaatat tgggcgcgat gaccatgtta
     7861 tttacggttt cagcctttgc agatgattgc gactcgatcc gtaaagctat caacgatttg
     7921 cccccggcag gcggcacggt cctgatcgac aaaggtattt acaattgtgg agcgccaatt
     7981 attatcaacc gcagcaacgt caacttaaaa ggagcgggtc aaaacaaagt cactctccgc
     8041 cttattgacc atgttcacgc tcccttgttg atcattggcg aacccaaaac agttatgaat
     8101 gaacacggaa attatgtggc agaacatcgg gtcgaaaata ttcgtgtctc cggtttaaca
     8161 cttgatggga accgcgcgaa ccatgatccc agtaaggaat gcggtgagac catttgtgaa
     8221 ggggatccaa gcgccgttcg caataacggc atcaccattc gcggcgctag caaggttctc
     8281 gttgaaaatg taaccaccca tagcatgatc tcggggggat tggtgacgga aaaatactgc
     8341 gatcaattga tcattcgcaa tttcaattct tacgataatt atttcgatgg ttttgcaggt
     8401 tatgaaacag agcacagcgt tctttccggg ctttacctac acaacaacag aggtgccgga
     8461 atctccatcg atatcaactt caataacaat atcatccgcg actcaaaatt acatgacaac
     8521 ggagacgtcg gaatttttgc gcgaaatctc agtgggaatc aattcaagcg cttaagcatt
     8581 cagcggagcg gcaaccacgg tgtgttttta gccagcgccg aggcccccaa ctcctgcgcc
     8641 cacgacaacc aattcgaggc ggttcttatc aatggctcga aacgctccgg ctttcgacta
     8701 aacaataatt gccagggaaa tcgcatcaca ggggcttcga atttatgtgg caacgccgaa
     8761 ggtggcttca gtgaagatcg ccaaggcacg ttgctcgtcg ctgataaagt cagctgccgc
     8821 taagtccatc tttttttgac agttcgctat ttcgcgaaat attatgaatt ccttaggggg
     8881 ttcataatgt ttcgaataaa ggcttgtttg ttttcttata tcttaatagc gctaacacct
     8941 tttgctgcga attcgcagca aagctctctt gaacagtgga tgaagacgga gggcaacatt
     9001 gctctcacgc gaatgaatca gaatatctct ccgacggggg cggcccgcgg ggtcgtcatc
     9061 gcctcccctt ctcgacagga tccggattac tattttcact ggactcgcga cgctgccttg
     9121 gtgatggaga ccttttacct ccctcaaact tttgcggatt atacggagtt ttctcgtcag
     9181 acccagttgg tctcgactct gactggcctc ggggagccgc gtttcaatat tgatggctca
     9241 gcctacacgg ggccctgggc acgaccgcaa aatgatggcc ctgcgctgcg ggtgctgacg
     9301 atattgcgaa gcctccgccg accgcagatt tcctctgcga cggcgcaagc caccgcacaa
     9361 accactgtac aagctattgt gcaaacagat ttgcaatata tcttgaatta tttccgcacc
     9421 ccctctttct gcctctggga ggaagtgaag ggagatcact tttacacccg acttgtgcaa
     9481 atggccgcgt tgaaagaaag tcttgagcat tttgattcca actggaatat tccccggcaa
     9541 actttgcaga cggcgatgac gagtttgcag aacgaactcg cccgccactg gcgcgacgac
     9601 aaaaaatact ttagtgcgac cttgaatcga atcgaaggtg ctgaaaacta caaattcacg
     9661 gatctcgaca cggcggtgat tttggctgtg cttcatgcga atctagagac tgatgaattc
     9721 tccgtaaaag atgatcggat tcttgcgaca gcctcacagt tggaaacggc atttgccgcc
     9781 acctatgaga tcaatgcgcc gacttatgca agcccctacc ccgtcgctcc cgctattggc
     9841 cgttatacgg atgacatcta ttttggcggc aatccttggt acatcacaac cgccgctttt
     9901 gccgaactcc attaccgctt gtctttgcag ttgatgaaaa acaattctta cgtcgtcacg
     9961 gaattaaacc tgccttttct caaaaaggct ttggctcttc gtgggtccga ctcggacgtc
    10021 gctgagctcc agagtggctt aaatctgaag acccgccctc agttggtgaa acttcttcaa
    10081 gaaggtttgc tcgccagagg cgatttgttt atggcccgac tgcgcctgca cacgcccgtt
    10141 gatggctcta tggctgagca gtttgcccgc aaagatggcc aaccggtttc agcccgggat
    10201 ttgacttgga gctacgcaag ttttttaagt gctcttaaat cgcgaaaagc actttttgaa
    10261 taagacacat aaatttttaa tgacagaatc gtcacacttt ggtcgagtta aagctcacaa
    10321 taagggggct ttgtgggttt aaagaattta attgttcttg tgacggccgc ttttttcact
    10381 cttcctgcaa cgacggcgct ggcgggtctc tatcgggttc accagaacca gctttggatc
    10441 gacaatgaac ctcaacccca actcttcggc gcggaggttc agtatttccg catgcgcggt
    10501 ggctatgaac gaaatattcc tcgcgatcgg gtgatggctc tttgggctca ggccttggat
    10561 cgcctggtcg aagcgaaaat gaatgtgatc agcttttata ttccctggga ttttcatgag
    10621 tatgctcccg gcaaatttga ttttgacgga acagcggatg aggatcacga cggaaatccg
    10681 gactaccctt cccgcgactt gcagactttt tttcagatga tcgaagccca cggcatccac
    10741 aaaatcatga ttcgtccggg tccctatatc aatgcggagt ggggctttct gggttttggc
    10801 gccgtgcctt tgtggtttca tgaaaaatac ccgcaaagcc atatgcaaaa tgcccagggc
    10861 ctttcgacca aactttatga ctatcacaac cctgatttgt tgagacacac ccgcttgtgg
    10921 tttgaggagc tttaccgcca agtcctgaaa aataaaattg gccctggcaa gcccatttct
    10981 tttttgcaga tcgacaacga aaccaacttc atgtggcagt cgttgtacaa tcacgattac
    11041 aatcctgcgg cactgaggcg ctatcgtgac tttttaaaat caaggtatca ggatcttgcc
    11101 actctcaatg ctcatcatca ccgcgcctgg tcccgctggg aagaggtgat ggcaccgacc
    11161 agtcccggcc tcaatatcgc cgaggatcag gattggtatc gctttgccga tgagtcgatt
    11221 tatctttatc tgcaaaaaat ccgcgctctt tgggaaaacc tcggcgtgcg cgaacccacc
    11281 gtcatgttca ctttagcgga cagctttaac gccactcaaa atggtttgct gccaaattat
    11341 caattgcaca acacgcccgg agtgactggc gtgatgaccg ttaatttgta tccgaaaact
    11401 tatgaaaccc ccgaacatcc gttgctgaat cttcctttca aagccgatca cgacgtcaag
    11461 gctgcggaag cggcctccga actttactgg ggaaacaagg aaagatgggt cctgggcccc
    11521 gagatccaag gcggttggtg gcgtggcatt gatgtcactt ccgaagctcg gcgacaaact
    11581 tatctcactg ttctcggaca tggcttgaaa gctttttttg tttattactt caacgaaggc
    11641 gacaactggc aatctgattg ggcgaaacag caaatccgtc ctctgtttga agaactgcgc
    11701 aaggaaccgc cgtttgtcaa cgtgagcgag gaccaattat ccgatgaatt ttggaagact
    11761 ttgcaagata gagtggatca ccgcatttta gtcggctttg atgtgcgcgg gattatgcac
    11821 gaagatcaga aaatcgcctc ccaactttat ttcgatgctc cgctggatcg tgaggctcag
    11881 ccgcgggatc attactttga gctcaaagaa ttgggcgaaa agctggtgac tccttacggc
    11941 cgctggctgg gtcgcgcggt tgagaccacg gatccggttt gcctgatcaa agacgtcaac
    12001 gatcatgttc ccagcgctgt ggctaatatc gacagcattc aaatgaacag cgactggagt
    12061 ggggggctca tcgcctatgc actccgaacg ggaatcaatg tgcgggtcgt ccattgggga
    12121 ctcaacgcca cagtcaatgc cttgtcggaa ttgcaggact gccgcctgat tctgcgacag
    12181 gatgccggcg gaacttccca ggccctcgcg gagaccttgc agaagctgat cacccaaggc
    12241 catactgtgg tgaattttct ggatgattcc ctcgccaaac aaatcggtat cgcggtgacg
    12301 aagacggttc agccaagcac cggaccggtt cttttgaact tcgagggaca gtcgttcgga
    12361 gcacagtcga gtcccttgtt tcattacaac accatggagg atgcgcgttg ttccgcagcc
    12421 atcacccatg agaccgcggc cgctgcttat cgctgcgcga ttggcaaagg aatgtttttg
    12481 caaatcggcg cactctttta tgatgtctat aattcaaatc aatatgccct gctttcggat
    12541 ctgtcgaatc gagagcttgt tttaaaaagc cttctgtctt cgctgaaaat cactcccgct
    12601 gttcatgtgg acgctgacaa agtggtcgcc tttgcaagac aagtcaaagg tgaggccccg
    12661 ctttgggtga ccgtaaaaag cgctcttcaa caagcaacgc tcacccatat tcaggttcgt
    12721 gatcttcaac caaaccagtt gtactcggtt cgcaatctct tgagtggtga acttcagcga
    12781 ctgcggggag caacgatcca acaaacaggt tttgcggcac agcttgcagc gtttggcagc
    12841 acggtattct ggcttgaacc catcagtatg acagcccaca gggagaaaaa ataatgatgc
    12901 gattcaaagg gacctggatc tttttaggac ttctcagcat tgccgcctgt tcaacacctt
    12961 cggttcgaga accctcgtca gcacctttac catcgcctat aaaaaagacg atggacatgg
    13021 ctcgtttgcg ctcagaagct tttacccgct gccagactcc cttttctcgg cctgtcgtag
    13081 aagctgttcc gattctcgat cctccccatg gcggagccga tgccgagggc atggtttacc
    13141 aagaggaact tgcggtcaga accacaaacc tgctcgacag agagggcttc tatttttcgc
    13201 cgcgaccgcg ggccgccgtc aacaccctgc ctatggagat ctttgttgat ggtgataata
    13261 ccggaaccta cgagcgaaaa acttgggaag atgcgaatcc agactactct tcgaatcaga
    13321 ttcagccacc catcttgcaa agcaatattc ccagcgctgg cagggtctta accgagctca
    13381 ttattggcca ccgctctccc aggggcgatt acccacagct ctttgacctt cgttcgagtg
    13441 cttactttcg cttcgcggga tatccgcctc aagtcacggg ggcttcgctc agactcgcag
    13501 ctcataagat ttttggtctc ggtggaaaag gtgaaaccgc cactaaagaa gatttcccta
    13561 ttgtccgagc catttataca ggaatcaaag attccaagac ggccaatgcc ctagtcctcg
    13621 ttgagagcga gttattttgc ggcgctttga acatggatat gctcgaaggt gataacccgg
    13681 aagtggtgat tgacagctac tggtacactc gcagcgattt caattggaag cgggatcccc
    13741 acaccggact cgtcgcctat agcagcatgc tttggaagac cgagaaacag acgccggaaa
    13801 gagtttctga tgaagcccat gactcggata tactgacggt gaaaaccgaa aatggggtgg
    13861 ttaaaaagta tccgctcgat cccccgaaaa ccggattgcg agtacgccat ctgaccccca
    13921 ctcaacaacc tgtggaatgg actctggcca acgaagaccg cgatccctcg cacttcgctg
    13981 actttcagcc cgccctcggc aatacaaatt acaactttcg cgcgagctat cgcgtgagcc
    14041 tgctggaaac caacgtaaga accggcgtga gcctttacga gcaagccgcc gacggcgagt
    14101 acggcgacaa cattgtcgcc gtttcaaccc ttcgtcagaa cattaaaaag gctacgaaag
    14161 tggatcagtt tatccggttc aagtataaaa ccacggcctt ctttgtcggc gataggtttt
    14221 gagagagata aatcagttag agctcctgct caaggcctat gtttttttaa ctttttcata
    14281 agttcatcat aagcctgtgg agccccttca gaaacgcacc agtctttaac tttcttttga
    14341 ttaaacggaa atctttgagc gaccaatacc gcttgatcta aacactcacg agccctgaaa
    14401 tgaatataag aggcaagtcg atctcgaata caatcggttg gagaataaat ataaagaatc
    14461 tgaccctgaa cctttttggc atcaggctta aaatggcagc tacagccccg ccaactaaaa
    14521 ttgtttcgat gccgttttgg ctaggtgcca cccaacgtac ttccaaactt cttcttctgt
    14581 gcacttttcc cacttgggct ctttcacaaa atgggctttc cttttcgacg gggccgccga
    14641 tgggtttgaa aaatagcctg acgttctgac agcgaaatcg cctcataagc aatctcaata
    14701 agcgctctca cgggtttgct caacggagat ttaggattga aagaataaac ccgcgacctg
    14761 ccaacttctt ttgaaaccaa aaccccggcc tgttcaaagc gatttaactg ctggataatg
    14821 gggtttaatg acattttaaa atcttttgct atgcccgagg cgtgagattc cccatagtgg
    14881 aacacatgaa gtaaaacttt ctctgctgtc ttattaccga atattccttc gagcatcaac
    14941 ttatcatctt ccagaatctg gtcgatatca accattttct ggttcataca gaatagaaac
    15001 tagcaccacg tcgcgggact gtatcttggc tcctagaatt aagataaaat ctgcaccaga
    15061 acgtagtcat catattttca caagcctttg aaaaaagtca cctgacatac tctggttgtt
    15121 ttccttgacg aagctcgatc tcattcctaa tgatgcttca aattcagcga ggaagagtat
    15181 gactaacaat attgcaataa tcttcagtgt tcttttattt tccttaggtc tctatgcgca
    15241 ggagaaatca ccgtggaccc acgaatccga ggcttccatc gtttctgtcg gtggcaatac
    15301 cacctcggag agctacagcg caaaacaaaa gacctcctat aagtttgact tgaatattct
    15361 gactgcttca gcaaggtatc tccagacaaa ggcctcaggc acagagaccg cgaaacaatg
    15421 ggatgccagc ttaagatatg aacgcgaact gagtgaaaat tgggctgtat tcattcagca
    15481 tggtgcagaa agtgatcctt acgctggata tgtgcaaaga gataattcgg atatcggcgg
    15541 gaaatactat ttcataaaga ccgacctcga aacactcctg tcggaagtgg gtcttcgagc
    15601 cactaaaaca attttgtcac caaccaatga ggttacttac accacttcgg gtcggcttta
    15661 cgttgaatat tcgaaaaaaa taaaccaatc ggtttctgga aaactttggg ccgagtattt
    15721 gcagaacttt aaagactcgg atgcctattt agtaaattac gaaccgtccc tgagtgtgat
    15781 gatgaatcaa atcttctctg ttaaactggc ttacttagtc aaataccaca ataaaaccat
    15841 cacaccggtc gagaaaaaag aagacacaac atttacgacg gccctggttg caaagttcta
    15901 atttaaaaca aaggtcgtgg agccggctgt acttgcaaat tgccaatcgc gatgagagag
    15961 tgggtaatca tgctcttacg cgcttcgatt tcagaaatta agcacggtac tgtcaccaaa
    16021 aaaaatggtt atcttgcggt caccatgagg atgtatccgc gtttcttggc aaagtcgtta
    16081 atgattataa acagtccctc tcacctcaaa aagaactttt cgaactttcg aagagccaag
    16141 atatctatat gatttgtcaa tgtgaacgaa acgaacgctg ccacgtggat ctcatgctgt
    16201 taattgcgaa aaagaatctg ggggcccgga tcggcgagct ggcctatgca tattctgagt
    16261 tctgtgaact tagacaatgg cagtatccta aaagtcatgg agattaagga attgtacctc
    16321 ctcaaccccc atcgggttat tgaacagatt ctggattacg catgatcaag atagccggaa
    16381 agttgacacc tctgaggtaa attcttagaa catagtgaga aatggaaggt ctatgattgg
    16441 tcgtattatt caattgagtc gtaaaactct cgatgtgttc ttcgttgttg caggagtgct
    16501 gtttcttttg attggcatcg ttggtattgt cctgccttta cttccaacaa caccgtttct
    16561 attactttcg gctttttgtt ttagtaaggg atctaagaag tttcatcact ggcttttaaa
    16621 tcactcgatt ctcggttcgc ccatccggga ttgggagcga aagaagatca tccgtaaaaa
    16681 atcaaaaatt ctggccactg tcatgatggc tggctcaagc gtttttgtcc tttccaaaga
    16741 taccattccc ctgattggga aagtttctta ttctttagtc cttatttcag ttctgatttt
    16801 tatctggacc cgtcgtagca ctgaggaact ttaggtcatg caaagggatc ggtcttccct
    16861 ttttgtcttt cctctattcg tgctacatac caagagctaa gcccagcggt tgtcagaatg
    16921 gcaaatatca caagcaatga gatccagctt gggattttga aaaaatccac caccaacatc
    16981 ttgatgccga taaatcccaa tatagccgcc aacccaggct tcagatagcg aagctttgcc
    17041 acccaatccg ccagcacaaa atagatggcc ctcaatccca aaatggccag aatattagag
    17101 gcaaaggcaa caaaagcatc ctgggttacg gcaaacaccg cgggaattga atccactgca
    17161 aaaaccagat cagtcacttc tatcacgacg agtgccagaa aaataggagt cgcttttctc
    17221 ttgccatctt caacaataaa aaagtggctt ccatttgatt tttccgttgt cgggatcaat
    17281 cgtttcaata agcgaaccga ccagttttct tgagcatcta tcttttcatc ggtttcacgt
    17341 aaaaacttaa tggctgtgat caccaaaatg aatccaaaaa tatacaatat ccagtggaaa
    17401 gcct
//
LOCUS       DATBTE010000455        21331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2456131, whole genome shotgun sequence.
ACCESSION   DATBTE010000455 DATBTE010000000
VERSION     DATBTE010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 21331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21331
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2456131"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="VIG33_17340"
     CDS             <1..1236
                     /locus_tag="VIG33_17340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY8272662.1"
                     /translation="KKKKEFRHLQCSEFSSPDKEVFMESKGERNFDSINISPLNAIEN
                     KTGRFLSLSVLLHIALFASIVLMTVPPIEFPKKEIVELEVEKDLIGNTAPIQAIPEGA
                     PIPETKGAAPTEPLAAAKVTTPISLPPLKTAIAPPPTSAPVPSSVVPVAPLPPATAAP
                     ISAAASPAALPETLDDIQTPPLETSEAGEVPLAQMDEKDLHENFEKVDQAHHKAVITA
                     KKSLDEEADKAAAEGDEAISEMDKETQDEAIAAALAAEKRRARDAAAIAAAEASEKAA
                     ADRATRLAADEGEGTGSKGSPAPTKEVAGIPGGVRSLEQLRQMAGNKFPQYDKDERLA
                     RQEGKAIFYAYVNKDGSLSQFKLGESSGYRNLDTKTLAALKKWKFHPGQEGWVEMPFK
                     WTLTGEAMEAGGQLRNSHK"
     gene            complement(1324..1452)
                     /locus_tag="VIG33_17345"
     CDS             complement(1324..1452)
                     /locus_tag="VIG33_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272663.1"
                     /translation="MASKTKKTEYIRERKKATSGTKRKAALRTKGTTKTAKALFKD"
     gene            complement(1488..2333)
                     /locus_tag="VIG33_17350"
     CDS             complement(1488..2333)
                     /locus_tag="VIG33_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272664.1"
                     /translation="MLNPLSVLKHSVLICLYFNLSSAQAQSFSSAATDAVGGAGRAAV
                     EAGDVNYLNPAGLVHLKGRYIYSTFNREDLSLSLSEATREVLVPASLSYFQRKMTDAL
                     QREIRWQDTRLSLSDFITDKLSMGITGILSSVKINELNYNQTNGNLGFFFTPTDKFGM
                     GWVFYNVFKGNENIPEEIRFQPRMGGGLNYIYKGFLRYRLDVLSADNNNFGKPAYMTG
                     IETLLNEWMVFRLGYQNDILAARELITEGVGFNGPVFSINYGHQGSIKGANFDRHSID
                     LLFSF"
     gene            2333..3445
                     /locus_tag="VIG33_17355"
     CDS             2333..3445
                     /locus_tag="VIG33_17355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY8272665.1"
                     /translation="MKYLCRFIYISWIVFSLLSYQRAFADANGFMAKKVQIGDNVISL
                     LKTHQFSEVERGRILSLVPELRSLYLQLDVSYLVKQELGFLEMRLYDPNKDLVFVIKK
                     IGHVIQAKMARAVFKTTVARVDGKVQGSLLASILERINSNWVASRFMDAYIMDYNLNR
                     GLARGAKFWLTVEKKYDGPYFIRYGEILQTSLEIHDQNVQKHFIKINSSGGVFIAERD
                     LIKNRPFYSPVSYLRVASLFQPNRRHPITRKIQPHLGIDFEMAEGSSIYAASTGFVVR
                     YGKSFSAGNYVVLLHANGAETTYNHMKRIEGKIRRGLKLVVGEKLGEVGCTGYCTKAH
                     LHFSLRQRGRMVDPLKFLRPYPVFAENLLHAKVAQF"
     gene            complement(3456..4748)
                     /locus_tag="VIG33_17360"
     CDS             complement(3456..4748)
                     /locus_tag="VIG33_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272666.1"
                     /translation="MRTKVRNTLLKSFSVVITVSVFIFAFQNCGKAGFDKVAEETIID
                     SSSKDTTAPFAYSASFDQITYNSCFGVGLGNNPVYFTLKAGAYDGGGVNLTPNFLSYV
                     KDNLKPSYPSTEVTVEQMKSFVGETVENASPTLQMALRTRGAPQQVRNPSGASPSLNK
                     DFVNLMSDLTDDRIMEPIFRALGGVVNYFPLAANQSQRVMEATLTYNADEGMAYSLRN
                     DLSNNGMLALTYTGPVGSSAFAARVPSSSSRIDSTGATVMDMTIAYGRGYNLTFAADI
                     APMTQLLHSGVTVAPNSRNPNNILVSIQESNLENPSASTGVIWTCAQARRYLIVKAED
                     AANYCPADPVDRMANANYRRELEIVRHHLPAQQWDVSIDRRCVVPKSGSCYKNVGGTA
                     EEPVEYDQTLACYQGIQGLADPSVAKRCAQYVTICLRN"
     gene            4887..5786
                     /gene="galU"
                     /locus_tag="VIG33_17365"
     CDS             4887..5786
                     /gene="galU"
                     /locus_tag="VIG33_17365"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEY8272667.1"
                     /translation="MSKIRKAIIPAAGLGTRFLPATKTVPKEMLTIVDAPIILYVVEE
                     AVKAGIEDIILISGRGKHAIEDFFDTSYELEDRLAKDGKSHLLDRMTKIRNLANIISI
                     RQKQALGLGHAVLSGLPVVGKEPFAVLLGDEITIGFHGEPNVTAQLVNSFNETGTSTV
                     AIMNVPEKDVSKYGIAEIKEQNSGFFKVSSLVEKPKPTETKSRWALPGRYVFDNEIMD
                     ILQNAKPSLGGEIQLTDSMKILCDRKGLNAMTFKASRFDAGDKLGYLQANIELGLRNP
                     ELQAGLQEYILQMSEKIKNGSLL"
     gene            5783..6622
                     /locus_tag="VIG33_17370"
     CDS             5783..6622
                     /locus_tag="VIG33_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272668.1"
                     /translation="MIMKILLLLLTPVFAYIPPTRMILQRTAENSGSGVYSIEQEVQF
                     NNAQDSLFLRETWVIENDRTMRVTITGTKDLKDQIKMQFVYAGGQRWNLNGSRENHRI
                     SEDFLEKYFNFRSTDHLANTLVNLKILPTNALAKKSFPTKNLENFKYEPEDFVRLSRA
                     GGVPNYAFGPPSPSEGASSPGIWIEQDQFLVRKFRLPSQVEVTADNYNQYARGLNYPK
                     IRTIRWGQNTVTIRLIGITAQRGGVASSLFQPNSLDISTKLDGLNNQPAKDAILEFYS
                     RFR"
     gene            6619..8673
                     /gene="priA"
                     /locus_tag="VIG33_17375"
     CDS             6619..8673
                     /gene="priA"
                     /locus_tag="VIG33_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEY8272669.1"
                     /translation="MIDQPLWRIAVEAPLPEALTYLQSPELPALQRGQLVNVPLGRRL
                     IKGLVLGPATTSLNPEIKLKAVHSLDEEYPILPEHFIKWLEWLSNYYIYPVGQVTQLA
                     YPPLPKTQKVRKSSRPPVIPNMESDIPPEMTQEQKFTFESIAGFKTFSSHLVFGVTGS
                     GKTEIYLRLLQKILEEGKSGLVLVPEISLTPQLINRFVRRFGDKIAAIHSQLTERERT
                     NQWWDIIEGRKSILIGARSALFCPIQNLGLIIVDEEHESSYKQDEKLKYHGRDAAVML
                     AKQADCPIVLGSATPSLETWKNAIESKYHLHTLKNRVANRSLPDMIVVDLRGEKSFEE
                     AQKLKQEKNPNVPYWMSPLLFDKMKVTLDAGDQCALFLNRRGIAQMVICPACGHTREC
                     PNCDINLTLHHHSHLVCHYCDYHESLKDKCPDCTEGHLQAMGLGTELLEKDLNTLFPG
                     KNVARADRDEIQNRADLENLIVSMETGTIDILVGTQMIAKGLDFPKLKLVGLVLADVG
                     FNLPDFRATERSFQLITQMSGRSGRHVKEGENPGGVIIQTFNPEHESLLYAQKHDFEG
                     FAQYELQNRAQLNYPPNGKLIGFRIQGTHLRGVQEAARLLAKRAQALKTQFPKYESVE
                     ILGPAEAPLAKLRGQFRFHLLLKGPVAAILNTFSRQALGDEEWLPTGIKILLDVDPMN
                     LL"
     gene            8777..9541
                     /locus_tag="VIG33_17380"
     CDS             8777..9541
                     /locus_tag="VIG33_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HEY8272670.1"
                     /translation="MKLQASGESEPGQVCVGCINELHRKAANSSGGVLMAQERAKEQH
                     RLSLWKNRVQLIKQARSCMNQKMYPEAAASYEKYLKIMEIIFECKKGDLSPALFKESA
                     RHTELTVVASVYWDLLRIYDMNHQYAERQSYCAKQLASFIRFTPIYPDIIRKAEIFQR
                     SAKNPAIIKQFLKMSAENRPRCFIATAAFENVYAIEVQQLRFFRDHNLKRISWGRGFI
                     KWYYHISPKIACLLDKHSWAKPIVRSLLRILIKCVR"
     gene            9595..10752
                     /locus_tag="VIG33_17385"
     CDS             9595..10752
                     /locus_tag="VIG33_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEY8272671.1"
                     /translation="MSQKKSQNHLYDYAIIGSGLSGLCIAAALSRFTDNIVLLESGDV
                     PGGMNRTIKFPTGTTNNGLRYIPDSERVHKALDFLENILGLKIAGESIEAPPVTYASG
                     TFKSFIGFGDNPPAFYEELNYFTANKMLNLKLEPYAWTQLLFEKFKGDFMSRSYVTKF
                     HALSNHATQLTINGAKTLNASQFIFCGSVKDLALLLPENVLAPRARQRLSKNTYWTAL
                     CLDICHSHNVTDSLAMHVLNGTTEDEIGPCVGRFLPAVQINDQTLQASQWMTFIEDEV
                     TEDSEVVGATLKKVKRQIKRAYPEALESIKLERIIVAPQIAGHGDLKLNANQSLAGLE
                     NLWIGSTAVNPQKNLIGALSQAQMIIAALGFDISAPEPSPQPAEASAGLLY"
     gene            complement(10780..11043)
                     /locus_tag="VIG33_17390"
     CDS             complement(10780..11043)
                     /locus_tag="VIG33_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272672.1"
                     /translation="MNWYVRDSDRESDVEKAKGRYPQAQFIENSPEWVNSHAGPDELF
                     YSDNKNNLEIVFQKSAKKVSAIRWEFSDSKLKLIRKPSSKAKF"
     gene            complement(11061..11615)
                     /locus_tag="VIG33_17395"
     CDS             complement(11061..11615)
                     /locus_tag="VIG33_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272673.1"
                     /translation="MNKIKSICMMSLIFLTDIAMGSPRATCAEASCPTGYYCAVGSSG
                     ALGCYKNEKTPQPDIPSCKNAICQKGYYCSVVSGAARCVKEENDENTSGNTTAPKEGC
                     GYNGFRNLRGELICPIGHYCEIDGLHTNGICKPAIRPGCLIHGHLVVECGWSMEKHSC
                     YGTECPQGTYCSANGSDSKPFCYE"
     gene            11793..11939
                     /locus_tag="VIG33_17400"
     CDS             11793..11939
                     /locus_tag="VIG33_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272674.1"
                     /translation="MKLLKEMFTIMIILAATGFAYSDKCDPLFGCPIDPGKCVRNPSS
                     CGVG"
     gene            12098..14035
                     /locus_tag="VIG33_17405"
     CDS             12098..14035
                     /locus_tag="VIG33_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY8272675.1"
                     /translation="MKNILFASFLALFASHNVLAKLEATTTTYLGLGKDSVSADDLKK
                     YAPPPLEPIAERTIREMLDISFPGAGLLTPDKKQFFFSWKITGVYQIWALDAQHPFPV
                     QLTGGKDSTRLEEITPDGKWLIISQDKDGAENPGIYLMSLDGMQRKVVFEDPKVHASM
                     AYISENAKFLVYFANNEKPDSYTYYKYDIASGKREKIFREDGQWFIADYTKNGRWLMV
                     KATTNTYREIYEYNLKSKELKPLLGQGEKEEFHVAYAKNEKDLLVITPKFGDFKKFYL
                     FKDHQWKEIFAPKSGEVEDFRLDQKKSRILLGTNENGYAKAYAFDAKTYKPIPLPKLP
                     NSENTYFGTTTWDGEVTMFSVSSYNTPRKSFAYNWKTKKLQQWVYPSTPGIDFNKFVP
                     AKLEYYSAQDGTKIPMFVRRPSKCETQACPVIVHFHGGPEGQSVPGFNSISQLYIDAG
                     FIFVEPNVRGSEGYGKTWLHADDQAKRLNVISDIEDAAKYIRTKWKITKIGVMGGSYG
                     GYSTLMAMTKFAGTYDAGVAIVGMSNLITFLQNTAPYRRALRVPEYGDLEKDHQALID
                     LSPVTYIDRVKSPLMIIQGVNDPRVPAGEALQIQKLLEKKNIPSSLILFADEGHGAEK
                     KSNQVLQTGHTIEFFKKYLLN"
     gene            14127..15998
                     /locus_tag="VIG33_17410"
     CDS             14127..15998
                     /locus_tag="VIG33_17410"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HEY8272676.1"
                     /translation="MAIQELNEDQLHTMSLEEKDAWWLKNVYKGDMPQLTLRSALTGM
                     LLGGVLSLTNMYIGIKTGWTLGVGISSVILSFAFFKLMSKLKLGSHMTILENNAMQSI
                     ATSAGYMTAPMMASIPAYMMVTGEVIPMWQTIWWIIALALLGVLFAFPLKKRFINDEQ
                     MPFPEGYAAGVVLDSLHSDDGESGTFKAKLLVAGAGLSALIEFLRADTILAALKIKFL
                     ALPHYWDDFIYNFVTPKIFGSPLKDLTIQFDTSIVMLGTGGLMSMKTSMSILIGGLLN
                     YFLCAPLLIQAGVIPEAKFKAIAMWALWGGAAIMTTSSLYSFFSKPQMIVDAFKKSFS
                     KRENKPDILAKIELPMWIFTLGIPVIGAIVVYIGHAWFGVHYWLGLLAIPLVFIFTLI
                     AVTATGLTAITPGSALGKLTQITYGILAPGNVTTNLMTAGITSEVSLNASNLLMDIKP
                     AYMLGGKPRHQALGHVLGIFAGAFVAVPVFYSLFHNDISLFTSDSMPLPGAAIWKGVA
                     EALTKGLGNLHPSAQAAAVIGATLGVVIEILNKKTRGRFPLSGVGLGLGFVLRFADSW
                     SMALGTLLFWLARKRYKDENSFGHRAFVENQETMAAGIIAGGSIIGIVLMLLENAVG"
     gene            16304..17254
                     /gene="rpsB"
                     /locus_tag="VIG33_17415"
     CDS             16304..17254
                     /gene="rpsB"
                     /locus_tag="VIG33_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY8272677.1"
                     /translation="MAQVTMKEMLDAGVHFGHQTQRWNPKMKNYVYTARGGIHIIDLQ
                     KTVVRAQKAADFVKEIAANGGRVIFVGTKKQAIEPIQEAAQKCGQYYVTKRWLGGMLT
                     NFETIKSSIARLRKIDQMKEKGEFNYLTKKERARFEKEYLRLADFLNGIRDMKEMPSA
                     MFVVDLPKEHIAVAEATRLGIPVVGIADSNSDPEIIQFPIPGNDDAIRSIKLFANMVA
                     DAYIEGSKIWEQKLRASSDKQSDLAKEEKGEIKEEAPKRRAPRPGAAAATTKPAKAAG
                     PAIVKATKARKLVAAGTAEDVEIQAELESAPVEGADETAE"
     gene            17387..18316
                     /gene="tsf"
                     /locus_tag="VIG33_17420"
     CDS             17387..18316
                     /gene="tsf"
                     /locus_tag="VIG33_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY8272678.1"
                     /translation="MSISATLVKELRERTSAGMMDCKKALEETKGDFDAAIEWLRIKG
                     LSAAAKKAGRIAAEGTVFAQTIGNTGILVEINSETDFVARNEGFKSLVLDIAEHITHA
                     ANVVGDILEHAFHKNPAKKISDIMTEATAKIGEKIAIRRFEKYTASTNSLVHTYVHGE
                     GKIGVLIEIAASNPAATTNPEMRAFAQDVCLHIAAMNPMAISSEQIPSDVVAKEKEVL
                     KAKNLEQGKKAEMIDKIVEGQIRKFLAENCLVDQAFVKNPDIKISDLAKEVGKKAGAD
                     LTIKRFARFELGAGIDKKVVDFATEVAEQMKGH"
     gene            18318..19031
                     /gene="pyrH"
                     /locus_tag="VIG33_17425"
     CDS             18318..19031
                     /gene="pyrH"
                     /locus_tag="VIG33_17425"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY8272679.1"
                     /translation="MASPAYKRILLKLSGEALAGSQGQGINNTVIKQIAQDVAEAFKV
                     GVEIGIVIGGGNIYRGVAASAEGMDRASADYMGMLATCINALALQDALEKENIPTRVQ
                     TAIEMAEIAEPYIRRKAIRHLEKGRIVIFGAGTGNPFFTTDTAASLRAMEISAEVIMK
                     ATKVDGIYDKDPKKFTDAKKFEKISYIDVLNKGLNVMDSTAISLCMDNKLPIITFDLT
                     QRGNVLRAVMGETIGTLVH"
     gene            19046..19603
                     /gene="frr"
                     /locus_tag="VIG33_17430"
     CDS             19046..19603
                     /gene="frr"
                     /locus_tag="VIG33_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY8272680.1"
                     /translation="MLADLKKNTLEKMEKTTKALVEELKKIRTGKAQVSMLDGVRVNY
                     YGNLSPLSQVASISCPDAKSFLIAPWESSILKEIESALVKSDLGMAPMNDGKVIRLKV
                     PDLTEERRKDLAKQVKKVAEDSRVAIRMVRRDANEIVKKALKDKTVSEDEAKKSETEI
                     QKITDDSIKKIDQIADEKEKSILTI"
     gene            19638..20363
                     /locus_tag="VIG33_17435"
     CDS             19638..20363
                     /locus_tag="VIG33_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011166088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEY8272681.1"
                     /translation="MTLPKHLAIIMDGNGRWAQVRRKPRIFGHIKGTRVAKKIITACS
                     RKKIKNLTLYAFSTENWLRPQPEVAFLMQLLRRYLRKETDNLIKENIRFSIIGDISKV
                     PDDVREAITKAMQATAKCTGLSLVFALSYGSRQEITEAVRTILAKVAAGEIKSTHIDE
                     SVVNAALSTYPTPDPDLIIRTSGELRLSNFLMWQAAYSEFYFTQTLWPDFSEKDLEKA
                     LQDYESRQRRFGGVADHNENISH"
     gene            20344..21162
                     /locus_tag="VIG33_17440"
     CDS             20344..21162
                     /locus_tag="VIG33_17440"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY8272682.1"
                     /translation="MKTFLIRTLSAVVALALLFAVYYSLQITGIKLICYFAVLIGGLE
                     LTHILLPRETGKFHKMLFYLILLVVFHVAANYASFAVMAFSLLTVVFLVISILAHGGF
                     QELESLVRYQATSILGFVYLGLLPSYAYRLLELKNGLVWFFTLLGVVFCGDIGAYLVG
                     ILIGKHKINPRFSPKKTWEGSMGGLLGSLIAGYVCSQYLPQIQIYTILTTAVCAGFVA
                     QFGDFFESLVKRVANVKDSGRLMPGHGGVLDRIDGILFASPLILIVASLSESLF"
BASE COUNT         6152 a         4777 c         4524 g         5878 t
ORIGIN      
        1 aaaaagaaaa aagagttcag gcatttacaa tgctctgagt tctcttcacc agataaggaa
       61 gtctttatgg agtccaaagg cgaaagaaat tttgattcaa taaatatttc tcccttgaat
      121 gctattgaga acaaaacggg aagatttttg tctttatcgg tacttttgca tattgcattg
      181 tttgccagta ttgttctaat gactgtacct ccgattgaat ttcctaaaaa agaaattgtt
      241 gaactcgaag ttgaaaaaga tctaattggc aacactgcgc ctatacaagc tatcccagaa
      301 ggggcgccta ttcctgaaac aaaaggcgct gctcctacag agcctttagc cgcggccaaa
      361 gtgaccacac ctatttcttt accgccactg aagaccgcga tcgctccgcc cccaacttcc
      421 gctcctgtgc cctcttctgt tgttccagtt gctcccttgc ctccagctac ggctgctcct
      481 atttctgcgg ccgcatctcc tgcagctttg cccgaaactt tagatgatat tcaaactcca
      541 cctttagaaa cttctgaggc tggtgaagta cccttggctc aaatggatga gaaggattta
      601 cacgaaaatt tcgaaaaagt agatcaggct caccacaaag cggtgattac tgctaaaaaa
      661 tctctggatg aagaggcgga taaggctgcc gctgaaggtg acgaagcaat aagtgaaatg
      721 gataaagaaa cccaggacga agccatagcc gccgccctcg cggcagaaaa acgtcgcgcg
      781 cgcgatgcgg ccgccattgc cgcggcagaa gcttcagaga aagcggctgc cgaccgagcg
      841 actcgccttg ccgccgatga aggagaggga acgggcagta aaggctcccc agcacctaca
      901 aaagaagttg caggtattcc cggtggggta cgttctttag aacaacttcg ccaaatggct
      961 ggtaataagt ttccacaata tgacaaggac gagcgtttgg ctcgacaaga aggtaaagcc
     1021 atattctatg cctatgtgaa taaagacggc tctctttccc aattcaaatt aggggagtct
     1081 tcaggatacc gtaatttaga tactaaaact ctggccgcac ttaaaaagtg gaaatttcat
     1141 ccaggccagg aaggttgggt cgaaatgccg tttaaatgga cgctgaccgg cgaagcaatg
     1201 gaagccggag gccaactccg aaattctcac aaataagcat gtgataaata atttcaataa
     1261 taaagacaaa caaaaaaagc cggatctacc ggctctttta ataatctcaa ttaaatcagc
     1321 aacttagtct ttgaaaaggg cttttgctgt tttcgtagtc cctttagtgc gaagggcagc
     1381 tttgcgttta gtaccgctag tagctttttt tctctcacga atgtattcag tcttttttgt
     1441 tttagaagcc atgattctct cccagtttta acgagtggtg ttagatatca aaaggaaaac
     1501 aacaagtcaa tggagtgccg atcaaaatta gcacccttta ttgaaccctg atggccataa
     1561 tttatagaaa aaaccggtcc attgaagccg acaccctcgg ttataagttc cctagccgcc
     1621 aaaatatcat tttgatagcc caaacggaag accatccact catttaacag tgtttcgatc
     1681 cctgtcatat aagccggttt accaaaatta ttgttatcag cagataggac atccaaacga
     1741 taacgtaaaa atcccttata aatatagttt agtccccccc ccattctggg ttgaaaacga
     1801 atttcttcgg gtatattttc attcccctta aatacattat agaagaccca acccattcca
     1861 aatttatctg tcggagtaaa aaagaaacct aaatttccat tggtctgatt gtaattcagt
     1921 tcattaattt tcacagagct taaaattccg gtaatgccca ttgatagctt gtcggtaatg
     1981 aaatccgaca atgacaatcg agtgtcttgc catctgattt ctcgttgaag cgcgtctgtc
     2041 atctttcgct gaaagtaaga tagacttgcc ggcaccaaga cttctcgagt ggcttcactc
     2101 agacttaagg atagatcctc tctattaaaa gtggaataaa tatagcggcc cttcaaatga
     2161 actaatcccg caggattcaa gtagttgacg tccccggcct cgaccgcagc ccggcccgct
     2221 ccgcccacgg catcggtcgc agcgctagaa aaactttggg cttgagccga actcaagtta
     2281 aaataaaggc atattaaaac agaatgtttt aacacgctta aaggattgag cattgaagta
     2341 cctctgccgt ttcatctaca taagttggat tgttttttcg ttgttatcct atcaaagagc
     2401 ctttgccgac gccaatggct ttatggcaaa aaaggtacag atcggcgaca atgtcatctc
     2461 tttgctgaaa actcaccagt tcagcgaagt tgaacgcggt cgtattcttt ccttagttcc
     2521 agagcttcgt tctctatatt tacaattaga cgtcagctat ctcgtcaaac aagagctcgg
     2581 attcctcgaa atgcggcttt atgacccaaa taaagactta gttttcgtta ttaagaagat
     2641 cgggcatgtc atccaggcaa aaatggctcg cgcggtcttt aaaacaaccg tggctagggt
     2701 ggatggcaag gtacagggct cccttctcgc cagtattttg gaaagaatca atagtaattg
     2761 ggtggcgtct cgctttatgg atgcctatat catggattac aatttaaacc gagggcttgc
     2821 tcgaggagct aaattctggc ttacggtcga aaaaaagtat gatggtcctt acttcattcg
     2881 ttacggtgaa atattacaaa cctctttgga aatccatgat caaaatgtgc agaaacactt
     2941 tataaaaata aattccagcg gcggagtttt cattgcagag agggatttga taaaaaatcg
     3001 tcctttttat tctccggtct cttacttacg ggtggcaagt cttttccagc caaatcgccg
     3061 acatcccatc accagaaaaa tccaaccaca cctgggcatt gattttgaaa tggctgaggg
     3121 ctcatccatt tatgcggcct ctacaggatt tgtcgtccgc tacggcaaaa gtttctcagc
     3181 aggaaattat gtagtactgt tacatgcaaa tggagccgag accacctaca atcatatgaa
     3241 acgcattgag ggtaaaatac gacggggatt aaagctcgtc gttggagaaa aattgggaga
     3301 agtcggatgc actggctatt gtaccaaagc tcatttacac ttttctttac gacaaagagg
     3361 acgcatggtg gatccgctca aattcctacg cccttatcca gtttttgcag agaatttatt
     3421 gcacgcaaaa gttgcacagt tctagttgct tttatttaat tacgaagaca aatcgtgaca
     3481 tactgagcac aacgtttagc aactgaggga tccgctaaac cttggatacc ctggtagcac
     3541 gccaaagttt gatcgtactc cacgggctct tccgccgtgc cgccgacatt tttgtaacaa
     3601 gatcccgact taggaaccac acaacgacgg tcaatgctca catcccattg ttgcgccggc
     3661 aaatgatgtc gaacaatttc gagctcgcga cgataatttg catttgccat gcggtccact
     3721 ggatctgctg ggcagtaatt ggccgcatcc tccgctttga cgatgagata acgacgagcc
     3781 tgagcacaag tccatatcac ccctgtagaa gctgatgggt tttctaaatt ggattcttga
     3841 atgctgacga gaatattgtt cggattacgg gagttcggtg caaccgttac tcccgaatgt
     3901 aaaagctgag tcataggtgc aatgtcggcc gcaaaagtaa gattataacc gcgaccgtaa
     3961 gctattgtca tatccatcac agttgctccc gtactatcaa tgcgtgaaga gctggacgga
     4021 accctcgcag cgaaggcaga acttccaact ggacctgtat aagttaatgc taacattcca
     4081 ttattggaaa gatcatttcg taaactatac gccattcctt catcggcgtt gtaggtaagt
     4141 gttgcttcca tcacccgttg actttggttt gccgcgagag gaaagtaatt cacgactccg
     4201 cctagcgctc taaaaatagg ctccatgatg cgatcatctg taaggtcaga cattaaattc
     4261 acaaagtctt tattcagact aggacttgcc ccggatggat tgcgaacttg ttgaggagcc
     4321 cctcgtgttc gcaatgccat ctgcaaagtg ggagaggcat tctcgaccgt ttctccgaca
     4381 aaagatttca tttgctccac cgtgacttcc gtcgaaggat aagaaggctt taaattgtct
     4441 ttcacatagc ttagaaagtt tggggttagg tttacgccac caccatcata ggcccctgct
     4501 ttcaaagtga aataaacggg attgttgcct aagcccacgc caaaacaaga attataggta
     4561 atctgatcaa agcttgcact ataagcaaaa ggagcagtgg tatctttact ggatgaatcg
     4621 atgatggttt cctcagcgac tttgtcgaat cctgcttttc cgcagttttg aaatgcaaaa
     4681 ataaacacac ttaccgtaat aacgacggaa aaggacttta agagagtatt acgaaccttg
     4741 gtcctcatga ttccccccag attagattaa catagtccgt cttaaatcct tgcttaaatg
     4801 caaagagcta tttcatttta aaacaaagta gaatcgcaac agtttaggac ttgtcttaaa
     4861 atgaaacgtt tgagatttat tagagcatgt ctaaaatcag aaaagcaatt atccccgcgg
     4921 caggtttggg aactcgtttc cttccagcga ctaaaaccgt ccccaaagag atgctcacta
     4981 tagtggacgc tcccatcatt ctctatgttg tcgaagaagc cgtgaaagcc ggaattgaag
     5041 atattattct tatctcaggt cgaggcaaac atgccataga agacttcttc gacacctcct
     5101 acgaacttga agataggttg gcaaaagacg gaaaaagcca tttactcgat cgtatgacta
     5161 aaatccgcaa cctcgccaat ataattagca ttcgccaaaa acaggcgctt ggcttaggtc
     5221 atgcggttct aagtggtctt ccggttgtcg gcaaagaacc ctttgccgtt cttctggggg
     5281 atgaaatcac catcggcttc cacggagaac ccaatgtcac agctcagttg gtaaattcct
     5341 ttaatgaaac tggcacttcc acagttgcga ttatgaacgt tcccgaaaaa gatgtctcta
     5401 agtacggaat cgctgaaatc aaagagcaaa actcaggctt tttcaaggtc agttcgctcg
     5461 tggaaaaacc aaaaccaact gaaactaaaa gtcgttgggc cctaccaggt cgctatgtat
     5521 ttgataacga aatcatggac attctccaaa atgctaaacc ctctttgggc ggcgaaatcc
     5581 agctcacgga tagcatgaag attttgtgcg accggaaagg tctgaatgct atgacgttta
     5641 aggccagccg ttttgatgct ggcgataaat tgggctatct ccaagctaat attgagctcg
     5701 gtttacggaa tcctgaattg caagcagggc tgcaagaata tattttacaa atgtctgaaa
     5761 aaatcaagaa cggatcgctt ttatgataat gaagatttta ttattacttc tgactcccgt
     5821 attcgcctac ataccgccca cccgcatgat tttgcaaaga acggcagaaa atagtggatc
     5881 tggagtttac tcgatagaac aagaagttca gttcaataat gctcaagact ctttattttt
     5941 aagagagact tgggtgatag aaaatgatcg caccatgcgg gtgactatca ccggcactaa
     6001 agatttgaaa gatcaaatca aaatgcagtt tgtgtatgca ggtggacagc gctggaacct
     6061 gaatggttcc cgagagaacc accgtatctc tgaagacttt ttggaaaaat attttaattt
     6121 ccgcagcact gatcatttgg ccaacacgtt ggttaattta aaaattcttc cgacgaatgc
     6181 cttggcgaaa aaatcttttc cgacaaaaaa tttagagaac ttcaaatatg aacccgagga
     6241 tttcgtacga ctttcacgtg ccgggggcgt tccaaattat gcttttgggc ccccttcacc
     6301 ttctgagggc gcaagttctc caggaatatg gattgagcaa gatcaatttt tagtgcgcaa
     6361 atttcgttta cccagccaag tggaggtgac tgcagacaat tacaatcaat atgcacgcgg
     6421 tctgaactat ccaaagattc gtacaatccg ctggggacaa aatacagtca ctattcgttt
     6481 aattggcata accgctcagc gtggtggagt cgccagctcc ctatttcaac caaattcttt
     6541 ggatatttcc acaaaactcg atggccttaa caatcaacca gccaaagatg ccatcctgga
     6601 attttattcg aggtttcgat gatcgatcag cctctctgga gaatcgcggt tgaggctccg
     6661 ctgccagagg ctctgactta tttacaatcc ccagagttac cggcattgca aagaggtcaa
     6721 ttagtgaatg tgccccttgg aaggcgcctc atcaagggac ttgttcttgg tcctgcaacg
     6781 acttctctca atccagagat taaactaaaa gctgtacata gtttggatga ggaatatcct
     6841 attctgccag agcattttat aaaatggctg gaatggcttt ctaattacta tatttatcct
     6901 gtcggccaag tgacccaact tgcctatcct cccctaccta aaacccagaa ggttcgaaaa
     6961 tcttcacgcc ccccggtgat tccaaatatg gaatccgata ttccgccaga gatgactcag
     7021 gagcaaaaat tcacctttga aagcattgcc ggttttaaaa cattctcttc gcatctggtc
     7081 tttggagtga cgggctcggg taaaactgaa atatatttgc gcttacttca aaagatcctg
     7141 gaagaaggca agtctggcct ggtacttgta cctgagattt ctctgacgcc ccaacttatt
     7201 aatcgcttcg tgcgccgttt tggcgataaa attgccgcca tccattctca acttaccgag
     7261 cgcgagcgca ccaatcaatg gtgggatatc atagaaggcc gtaaatccat tctcataggc
     7321 gcacgctcag ctcttttttg tccaattcaa aatttaggtt tgattatcgt cgatgaagaa
     7381 catgaatcca gctacaaaca agatgaaaag ctcaaatatc atggccgaga tgctgctgtc
     7441 atgttagcga aacaagccga ttgtccaatt gttcttggtt ccgcaactcc aagcctggag
     7501 acctggaaaa atgccattga atcaaaatat catcttcata ctttgaaaaa ccgcgtcgcc
     7561 aatcgttctt tgccagacat gatcgtggtt gatctgcgag gcgagaaatc ttttgaagaa
     7621 gcacaaaagt taaaacaaga aaaaaatcct aatgtacctt attggatgag tccgctgctg
     7681 tttgataaaa tgaaagtcac tcttgatgcg ggagaccagt gcgctctgtt tctgaaccga
     7741 cgaggaatcg cgcaaatggt gatctgtcca gcgtgtggac atactcgcga atgcccgaat
     7801 tgtgacatta acttaactct gcatcatcat tctcatttag tctgccacta ttgtgattat
     7861 catgagagtc tcaaagataa gtgtccggac tgtacagagg ggcatttaca ggccatgggg
     7921 ttagggaccg agcttctgga aaaagatctt aatacacttt tcccaggcaa aaatgtggct
     7981 cgtgcagacc gtgatgaaat tcaaaatcgc gcggatttag aaaatctcat cgtaagtatg
     8041 gaaaccggaa ccatagatat tctcgtcggc acacaaatga ttgctaaagg cctcgacttt
     8101 ccgaaattaa aattggtggg ccttgttctt gcggatgtgg gtttcaatct tccggatttc
     8161 agagcaaccg aaagaagttt tcaattaatt actcagatga gcggtcgctc gggtcgtcat
     8221 gtgaaagagg gcgaaaatcc tggcggtgtt atcattcaaa catttaatcc tgaacatgaa
     8281 agcttacttt acgcacaaaa acatgacttt gaaggttttg cgcaatacga acttcaaaat
     8341 cgcgctcaat tgaattaccc accaaacgga aagcttattg gtttccgtat tcaaggtacc
     8401 cacttgcgag gtgtgcaaga ggcagctcga cttttggcta aaagagcaca agctttaaaa
     8461 acacaatttc ctaaatatga atctgtagag attttaggtc cggcagaagc gccccttgca
     8521 aaactccgtg gtcaatttcg ctttcatttg cttctgaagg gtcctgtagc agcgattctt
     8581 aatacttttt ctcgacaagc actaggcgat gaagagtggc tgccgaccgg aataaaaatc
     8641 ctcctcgatg tagacccaat gaatttactc taatcttgga gaatctagaa taaggttgta
     8701 acatagttcc atgattcaat gtccccgttg tcgaattcaa gttactgaac tgcactctgt
     8761 ccctggtgac cttgcaatga aattgcaggc ttcgggtgaa agtgagccgg ggcaagtttg
     8821 cgtaggctgc attaatgaac ttcaccgcaa agctgccaat agcagtggcg gcgttttgat
     8881 ggcccaagaa cgcgcgaaag aacaacatcg actttctctt tggaagaacc gcgtgcaact
     8941 gattaagcag gcgcgcagct gtatgaatca aaaaatgtat cccgaagcag ccgcttccta
     9001 tgaaaaatac cttaaaatta tggaaattat ttttgagtgc aagaaaggtg acctcagtcc
     9061 agcccttttt aaagaaagcg ctcgccatac cgaactgacc gtcgtggctt cagtctattg
     9121 ggatctgtta cgtatttacg acatgaacca ccaatatgca gaacgccaaa gttattgtgc
     9181 taaacaattg gccagcttta tccgcttcac acctatttat cctgatatca ttcgcaaagc
     9241 cgaaattttt caaaggtccg ccaagaatcc tgcgataatt aaacaattcc ttaagatgtc
     9301 agcggaaaac cgccctcgat gctttattgc caccgccgct tttgaaaatg tttatgcgat
     9361 agaggttcag caattgcgtt tttttcggga tcataattta aaaagaattt cctggggtcg
     9421 tggctttata aaatggtatt atcatatctc ccccaaaatc gcttgcctac tcgacaagca
     9481 ttcttgggca aaacccatcg ttcgcagcct tcttcgtatt ttgattaaat gcgttaggta
     9541 gtttcaatca tctttgcact tgcaaagcct ctcggggtta gtgaaggtag aaatatgtct
     9601 caaaaaaaat ctcaaaatca tctctatgac tatgcaatta tcggaagcgg gctcagcgga
     9661 ttgtgtattg cagcggcttt aagtcgcttc actgacaata tcgtgttact cgaaagcggg
     9721 gacgtgcctg gcggcatgaa tcgcaccatt aaatttccaa ctgggacaac gaacaatggc
     9781 ctccgctata ttcctgactc tgaaagagtt cataaagccc tcgatttcct agaaaatatt
     9841 ttagggctta aaatcgctgg cgaaagcatc gaagctcctc cagtgacata tgcctctgga
     9901 acattcaaat ccttcattgg atttggtgac aatccccctg ccttttatga agaattaaat
     9961 tacttcacag cgaataagat gctgaaccta aaactcgaac cttatgcctg gacccaattg
    10021 ctttttgaaa aattcaaagg tgattttatg tctagatctt acgtgacaaa gttccatgca
    10081 ttgagcaacc atgccactca gctcactatt aatggagcaa aaactttgaa cgccagccaa
    10141 tttatttttt gtggttcggt gaaagatctc gcattgcttt tgccagaaaa tgtgctcgct
    10201 ccccgtgccc gccaacggct cagcaaaaac acttattgga ccgctttgtg tcttgatatc
    10261 tgccattctc ataacgttac ggatagttta gcaatgcatg ttctcaatgg aactaccgaa
    10321 gacgaaattg gtccctgtgt cggcaggttt ttaccagcag ttcaaataaa tgaccaaact
    10381 ctgcaagcct ctcaatggat gacttttatt gaagacgaag tcaccgagga cagtgaagtg
    10441 gttggcgcca ctttgaaaaa agtaaagcgc caaataaaac gcgcttatcc cgaggcgctc
    10501 gagtccataa aattagagcg aattatcgtc gcaccacaaa ttgcaggtca tggcgattta
    10561 aaattaaatg caaatcaatc attagctggt cttgaaaact tgtggattgg ctctacagca
    10621 gtgaatccgc aaaagaacct tatcggggct ctttctcagg cgcaaatgat catcgcagct
    10681 cttggattcg atatcagcgc gccagagcct tcgccgcagc ctgcagaggc ttccgcaggt
    10741 ctcttatact agagactcct ctgaaggatt cgcgatggtt tagaatttag cttttgagga
    10801 aggctttcta ataagtttaa gttttgagtc tgagaactcc caacgaattg cacttacttt
    10861 ttttgcactc ttttgaaaaa ctatttctaa gttattcttg ttgtcggaat aaaaaagttc
    10921 atcaggacca gcgtgagaat tcacccattc cggagaattt tcaataaact gcgcttgcgg
    10981 ataacggcct ttagcttttt caacatcgct ttctctatct gaatctctca cgtaccaatt
    11041 cactatattt agtcatccta ctattcataa caaaagggct tactgtccga tccgttagca
    11101 gaacaataag tgccctgagg gcattccgtg ccataacagc tatgtttttc catagaccat
    11161 ccacattcaa ctacaaggtg accatggata aggcaacccg gtcttattgc tggcttgcat
    11221 attccattcg tatgcaaacc atctatttcg caataatgac ctattggaca gatgagttct
    11281 ccacggagat ttctgaagcc attgtatccg cacccctctt tcggtgccgt tgtatttcct
    11341 gatgtatttt catcgttttc ttctttcaca caacgggcgg ctccgctcac caccgagcaa
    11401 taataccctt tttgacaaat ggcattttta catgaaggaa tatctggctg aggagttttt
    11461 tcgtttttgt agcagcctaa agcgcccgag ctccctaccg cacaataata acctgtagga
    11521 caggaagcct cggcacaagt tgcacgagga gaacccatgg ctatatctgt taaaaaaatg
    11581 agactcatca tgcaaatact tttaatctta ttcaaaccgt ccccccccct agtgataagt
    11641 tagggggaca gtaatatttt ttatattctt gaagtcaaaa gcaaatgcag gacattaaaa
    11701 cttatttgtt tttcgcttga aggtatagac acctttcaaa ctctatgttg taactttcta
    11761 ctctgtaact aaccttgaat ttgggagaag cgatgaagtt acttaaagaa atgttcacga
    11821 tcatgattat tttggcggca acaggttttg cctatagcga taaatgcgac cctctttttg
    11881 gatgccctat tgatccggga aaatgtgttc ggaatccaag cagctgcggt gttggataaa
    11941 ggagtggggc cttacgggtt cgagactacc ctccccaatc tctcatctca catgcttcag
    12001 ctcattagat tcaaataaga aatattctag acccattgac cgtatcctgt ggtccatggc
    12061 tttgatattc ttcataagat taaatggaga tcatcttatg aagaatatct tgttcgcttc
    12121 tttcttagct ctttttgcaa gccacaacgt tctcgcaaaa ttagaagcca ccaccaccac
    12181 ctatttaggt cttggcaaag attctgtctc tgcagatgac ctgaaaaaat atgccccgcc
    12241 gcctttagaa cccattgcgg agcgaactat tcgcgagatg ttagacatct cctttcctgg
    12301 agccggactg ctcaccccgg ataaaaaaca attctttttt tcttggaaaa tcaccggtgt
    12361 atatcagatt tgggctttag atgcccaaca tccgtttcca gttcagctga ccggcggcaa
    12421 agactccact cgtcttgaag aaataactcc tgatggaaag tggcttatca tcagccaaga
    12481 taaggacggc gcggaaaatc ccgggatcta tttaatgagc ttagacggaa tgcaaagaaa
    12541 agtcgttttc gaagacccca aagtccacgc ttccatggcc tatatctctg agaatgcaaa
    12601 gtttttagtc tatttcgcga ataacgaaaa gccggatagc tatacttatt ataagtatga
    12661 catcgcttct ggaaagcgtg agaaaatttt ccgtgaagat ggccagtggt ttattgcaga
    12721 ttatacaaaa aacggccgct ggctgatggt caaagccacg accaatactt atcgtgagat
    12781 atatgaatac aatctcaaaa gcaaagaact aaaacctctt ctcggccaag gcgaaaaaga
    12841 agagttccac gtggcctatg ccaaaaatga aaaagatctt ttagtgatca ccccaaagtt
    12901 cggcgatttt aaaaaattct atctttttaa agaccatcaa tggaaagaaa tttttgcacc
    12961 caagtccgga gaggttgaag atttccgtct tgatcaaaaa aaatcgcgca tcctgctcgg
    13021 caccaatgaa aatggttacg caaaagccta tgcttttgat gctaagacct acaagcccat
    13081 tcctttacca aagctaccaa acagtgaaaa tacctatttc ggcactacga cttgggatgg
    13141 tgaagtcaca atgttttcgg tttcctctta taacacccct cgcaaaagct ttgcttacaa
    13201 ttggaaaact aaaaagttac aacaatgggt ctacccaagc actccaggaa ttgattttaa
    13261 taaatttgta cctgcaaagc tagaatatta ctcggctcaa gatggaacga aaattccaat
    13321 gttcgtacgt cgaccctcca agtgtgaaac gcaagcctgc ccggtcattg tccactttca
    13381 tggtggccct gaggggcaaa gtgttcccgg ctttaattcc atttcccaac tttatattga
    13441 tgcaggattt attttcgttg aacccaatgt tcgcggcagc gagggttacg gaaagacctg
    13501 gctgcatgcc gatgaccagg caaaaagact caatgttatt agcgacattg aagatgccgc
    13561 aaaatatatt cgcactaaat ggaagatcac caaaatcggg gtcatgggcg gcagctacgg
    13621 tggctattcg actttgatgg caatgactaa attcgctggc acctatgatg ctggcgttgc
    13681 catcgtcggc atgagtaacc tcatcacttt cttgcaaaac accgctccct accgccgcgc
    13741 tttgcgtgtc ccagaatacg gggatcttga gaaggaccat caagccctga ttgacctttc
    13801 ccccgtcact tatattgatc gtgtcaaatc accgctaatg attatccaag gagtgaatga
    13861 tccccgcgtc ccagcgggtg aggccctgca aattcaaaag ttgctggaaa agaaaaatat
    13921 cccttcgtca ttaatcttat tcgcagatga agggcatggc gccgagaaaa agagcaacca
    13981 agttttacag actggccaca ctatagagtt ctttaaaaag tatttgctca attaaaaaaa
    14041 gaacaacact cctttcgctg cgctcaagaa ttcagtgacg ccacaacttc aagtgaggta
    14101 taaacccaag cactcacgga ggatttatgg ccattcagga acttaatgaa gaccagcttc
    14161 acacgatgtc tttggaagaa aaagatgcgt ggtggctgaa aaatgtttac aaaggcgaca
    14221 tgccccaact caccctgcgc tccgctttaa ctggaatgct ccttggggga gtgctcagtc
    14281 tgacgaatat gtacatcgga attaaaaccg gttggactct tggtgttgga atctcctcgg
    14341 tgatcctgtc ttttgctttt ttcaagctga tgagtaagct caaacttggc tcccacatga
    14401 ccattttaga aaacaatgcc atgcaatcta tcgcgaccag tgcaggatat atgacagcgc
    14461 ctatgatggc ttcaatcccg gcatatatga tggttacggg cgaagtcatt ccgatgtggc
    14521 agactatctg gtggattatc gccttggcat tgcttggagt ccttttcgca tttcctctca
    14581 aaaaacgttt tattaatgac gaacaaatgc ctttcccaga gggctatgct gccggcgtgg
    14641 tgttggacag tctccactct gacgatggag aatccggcac attcaaggcc aagcttctag
    14701 tcgcgggggc tggcctttcc gcactcattg aatttcttcg agctgataca attctagcag
    14761 ctcttaaaat taaatttctt gcactgcccc actattggga tgatttcatt tataacttcg
    14821 tcactccaaa aatcttcggc agccccctta aagatctcac cattcagttt gatacttcga
    14881 tcgtgatgct tgggaccggc ggattgatga gtatgaagac ttccatgtcg attttaatcg
    14941 gcggtttact taattatttc ctctgcgcgc cccttcttat tcaggccgga gttattcccg
    15001 aagcaaaatt taaagctatt gctatgtggg cactttgggg aggggccgcg atcatgacca
    15061 cctcatctct ttattctttt ttctcaaaac cccaaatgat cgtggacgct tttaagaaaa
    15121 gcttttcaaa aagagaaaac aaacctgata ttctagctaa aattgaattg ccaatgtgga
    15181 tcttcaccct gggaattccg gtgatagggg ccatcgtagt ttatattgga cacgcctggt
    15241 ttggagttca ttactggttg ggacttctcg cgattcccct tgtctttatt tttacattaa
    15301 tcgctgtgac cgcaaccggc cttacggcga tcaccccagg aagcgcgctt ggaaagttaa
    15361 cgcaaatcac ttacggaatt ttggctccag gaaatgtaac cacgaacttg atgacagccg
    15421 gaatcacttc agaagtttcc ttaaacgcca gcaatctctt aatggatatc aagcccgctt
    15481 atatgctcgg aggcaagccc cgccatcaag cgcttggtca cgttctaggt attttcgccg
    15541 gagcctttgt cgccgtgcca gttttctatt ctcttttcca caacgacatt tcgcttttta
    15601 cttcggacag tatgccgctc ccgggagccg caatttggaa gggcgttgcc gaggccctga
    15661 cgaaagggct tggcaacctg catccctccg ctcaagcggc ggctgtcatt ggcgccactc
    15721 taggagttgt gattgaaatt cttaacaaaa aaactcgtgg aagattcccc ctttccggcg
    15781 tcggcctcgg cttaggattt gttcttcgtt tcgcagactc ttggtcgatg gccctgggaa
    15841 ccctgttgtt ttggctcgct cgcaagcgct ataaagatga aaatagtttc ggtcatcggg
    15901 ccttcgttga aaaccaagag accatggctg cggggattat tgctggtgga tccatcattg
    15961 gaattgtcct tatgttgctc gaaaatgcag taggttgatc ttaaaaaatc ccccaagaac
    16021 cttcccaggg gattttgttt tttgaaagtc ttccgttcaa ccccgataaa ctagctttta
    16081 tgaggccgtg atgaagttta actcaatgat tttattgaat ttataaatat acgctggaat
    16141 tcagaataga agcttgcccg aaagcgctgc tccgtgatat ccccaatggc aactataact
    16201 cacttttcga taaacactgg tcccgcaata tgggaattac ttgtttctcc tccgaggaac
    16261 aagctctgtc gaaagggtgg ctaaccagat aaaaggagag accatggctc aagtaaccat
    16321 gaaagagatg ctcgacgcag gcgttcactt cggacaccaa acccagcgtt ggaacccaaa
    16381 aatgaaaaac tacgtataca ctgctcgcgg aggcattcat attattgacc tccaaaaaac
    16441 agtggttcgc gctcaaaaag ccgctgattt cgtaaaagaa atcgctgcta acggtggccg
    16501 cgtgatcttc gtgggaacta aaaagcaagc tattgagcct atccaagaag cagctcaaaa
    16561 atgcggtcag tactacgtta ctaaacgttg gttgggcggc atgcttacta atttcgaaac
    16621 tatcaagtct tctatcgctc gccttcgtaa aatcgatcaa atgaaagaaa aaggcgaatt
    16681 caattatctt actaaaaaag agcgagcacg ttttgagaag gaataccttc gtttggcgga
    16741 tttcttaaac ggtatccgtg atatgaaaga aatgccttct gcaatgttcg tggtagactt
    16801 gccaaaagaa catatcgccg ttgctgaggc gactcgctta ggcattcctg ttgttggaat
    16861 tgcagactca aactctgatc cagaaatcat tcaattccct attccaggaa atgatgatgc
    16921 tattcgttcc attaagcttt tcgctaatat ggtagctgat gcttatattg aaggttcaaa
    16981 aatttgggaa cagaaactcc gtgctagctc agacaaacaa tctgatcttg ctaaagaaga
    17041 aaaaggcgaa attaaagaag aggctcctaa gcgccgcgca cctcgtccag gagcagcagc
    17101 tgctactacg aagcctgcga aagccgcagg acctgcaatt gttaaagcga ctaaagctcg
    17161 taagctagtt gctgccggaa cagctgagga cgtagaaatc caagctgagc ttgaaagcgc
    17221 accagttgaa ggcgctgacg aaacagctga gtagtctaaa agaacaggcg aagcagctgg
    17281 tctataatct aggtttataa tctataagta aaagccagct gtttctttgt gacccaagtt
    17341 tcttatatct gattataaaa tttcatgtat tgaaggagaa cttctaatgt caatttcagc
    17401 cactctcgta aaagaactca gagaaagaac tagcgccgga atgatggact gtaaaaaagc
    17461 ccttgaagaa accaagggtg atttcgatgc ggctatcgaa tggctccgta taaaaggcct
    17521 ttccgctgct gctaaaaaag ccggtcgtat tgccgcagaa ggcactgtgt tcgcacaaac
    17581 tatcggcaac actggtattc ttgttgaaat caactcagaa acagattttg tagcccgcaa
    17641 tgaaggtttt aaatcccttg ttttggatat cgctgagcac atcactcatg cagccaacgt
    17701 tgttggcgat attcttgagc atgctttcca taaaaatccg gctaaaaaaa ttagcgatat
    17761 tatgactgag gcgacggcaa aaatcggtga gaaaatcgca atccgtcgtt ttgaaaaata
    17821 caccgcatct acaaatagcc ttgtgcatac ttatgttcac ggtgaaggta aaatcggcgt
    17881 gttgatcgag atcgccgctt caaatcctgc ggcaactaca aatccagaaa tgagagcttt
    17941 cgctcaagat gtttgcttac atatcgctgc aatgaaccca atggcaattt cctctgaaca
    18001 aattccttcg gatgttgttg ctaaagaaaa agaggttttg aaagcaaaaa acttggaaca
    18061 aggtaaaaaa gctgaaatga tcgataagat cgttgaaggt caaatccgta agtttttagc
    18121 agaaaactgc ttagtagacc aagctttcgt aaaaaatcct gatatcaaaa tcagtgattt
    18181 agcaaaagaa gtcggtaaaa aagctggagc tgatttgact atcaaacgct ttgctcgttt
    18241 cgagctcggt gctggtatcg acaaaaaagt tgtcgacttc gcaacggaag ttgcagaaca
    18301 aatgaagggt cactaatttg gcatcccctg cttacaaaag aattttgcta aaattgagtg
    18361 gtgaggcctt agctggtagc caaggtcaag gcatcaataa caccgtcatt aagcaaatcg
    18421 ctcaggacgt ggcagaggct ttcaaagtag gcgtcgaaat tggaatcgtg attggcggtg
    18481 gtaacatcta tcgaggtgtt gccgcttccg ctgaaggcat ggatcgcgcc agcgctgact
    18541 atatgggcat gctggcgact tgtatcaatg ccctcgcact tcaagatgcc ttggaaaaag
    18601 aaaatattcc cactcgcgtg caaacagcta tagagatggc tgaaattgcc gagccctata
    18661 ttcgtcgcaa agcgattcga catcttgaaa aaggccgcat cgttatcttt ggagctggta
    18721 ccggaaatcc tttctttaca accgatacag ccgcttcttt acgggctatg gagattagcg
    18781 cggaagttat catgaaggcc acgaaagtgg acggcatcta tgataaagat ccgaaaaaat
    18841 tcacagatgc taaaaagttc gaaaaaatca gttatattga tgtccttaat aaaggcttaa
    18901 acgtgatgga ttcaacggcc atcagccttt gtatggataa taaactccca atcattactt
    18961 ttgatcttac ccaacgtgga aacgtgctaa gagccgtcat gggtgaaacc atcggtacgc
    19021 tcgttcacta acagaggaga cagtcgtgct tgcagattta aagaaaaata ctcttgaaaa
    19081 aatggaaaaa acgaccaagg cattggttga agaacttaaa aaaatccgga caggtaaagc
    19141 ccaagtctcc atgcttgatg gcgttagagt gaattactat gggaatcttt cgcccctttc
    19201 tcaagtggct tctatttcat gcccagacgc taaatctttc cttattgcgc cctgggaatc
    19261 ctctatttta aaagaaattg aaagtgctct ggttaaaagt gatcttggaa tggctccgat
    19321 gaatgacggt aaagtgatcc gtttaaaagt tccagatctc acagaggaac gccgtaaaga
    19381 tttggcaaaa caagttaaaa aagttgccga ggattcaagg gtcgcaatcc gtatggttcg
    19441 tcgggacgcc aacgaaatcg ttaaaaaagc cctaaaggat aaaactgtca gcgaagatga
    19501 agctaaaaag tctgaaacag aaattcagaa aataactgac gattctatca aaaaaattga
    19561 tcaaattgct gatgaaaaag aaaagagcat tttgactatc taagcttcat tacttgctta
    19621 ttttttctgg agtcccaatg actttaccaa aacatcttgc aattattatg gacggaaatg
    19681 ggcgctgggc tcaggttcgc cgtaagccac gtatttttgg tcatatcaaa gggacccggg
    19741 tcgccaaaaa aattatcact gcgtgctctc gtaaaaaaat taaaaactta acactctatg
    19801 ctttcagcac tgaaaactgg ttaagacctc aaccggaagt tgctttctta atgcaacttc
    19861 ttcgccgcta tctcagaaaa gaaactgaca acttaataaa agaaaatatt cgcttttcca
    19921 ttattggaga tatctcgaaa gttcccgatg atgttcgtga ggcgataacg aaagctatgc
    19981 aggcaacagc gaaatgcaca ggacttagcc ttgtctttgc cctcagctat ggctctcgcc
    20041 aggagataac agaagctgtg cgtactattc tcgccaaagt agctgctggt gaaattaaat
    20101 cgactcatat agatgaatcc gttgttaatg cagccctcag tacttatccc acacctgatc
    20161 cggatttaat tattcgcacg agtggtgaac tccgtctctc taatttttta atgtggcaag
    20221 ctgcctacag tgaattctat tttactcaaa cactatggcc ggacttttcc gaaaaagatc
    20281 tcgaaaaggc gcttcaagat tatgaaagta gacaaagaag gttcggagga gttgcggatc
    20341 ataatgaaaa catttctcat tagaacgctt tctgcagttg tcgctttagc tttacttttt
    20401 gcagtatatt attcattgca gataactggc attaaactca tttgttattt cgcagttctt
    20461 ataggcggtc ttgaacttac tcacattcta ttgccccgcg aaactggaaa attccataaa
    20521 atgcttttct atttaattct tttggtagtt ttccatgtag ctgctaacta tgcctctttt
    20581 gcagtcatgg ctttttcttt attaactgtg gtttttctcg ttatcagcat tcttgcccat
    20641 gggggtttcc aagagcttga atcactagtt cgctatcagg cgacttctat attaggattt
    20701 gtttacttag gactcttgcc ttcatatgct taccgcttat tagaactgaa aaatggtcta
    20761 gtttggttct ttactctctt aggagtcgtt ttttgtggag atatcggagc ctatttagtc
    20821 ggtattctta taggaaaaca caaaataaat cctcgttttt cccctaagaa aacctgggaa
    20881 ggatctatgg gcggtctcct gggttctctg atcgctggat atgtatgttc ccagtatctt
    20941 ccgcaaattc aaatatatac gatactaacc accgcagtgt gtgcaggatt tgtcgctcaa
    21001 tttggagatt tctttgaatc acttgtcaag cgagtcgcaa atgtcaaaga ttccggacgt
    21061 ttaatgccgg gacatggtgg cgtcttagac cgaatcgatg gtatcttatt tgccagccct
    21121 cttatattga ttgtagcttc tctttctgag tcattgtttt aaacaacaag gccgatgtct
    21181 aggtctaatc cctaattcgc cataccaatc cattaatttc ctagctgata tggtgccgtc
    21241 ttagtaaaat aggaacatgg aaattatact cgcccttttt cacaaagcgt tctcttcttt
    21301 cgtacctttt gtcatccttt tgggtatttt g
//
LOCUS       DATBTE010000456         3319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2250028, whole genome shotgun sequence.
ACCESSION   DATBTE010000456 DATBTE010000000
VERSION     DATBTE010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2250028"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1789
                     /locus_tag="VIG33_17445"
     CDS             38..1789
                     /locus_tag="VIG33_17445"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEY8272683.1"
                     /translation="MRLFFVGLIFIMTLSAQAREFSLKIEANSISSLSPEERNAIHSS
                     LEAVEGLLPTKIKEALAPQVTLRFPLLSSEKIREKWERWIRGILLREIFRLYDDKNHT
                     LSDHPVFLTAAGWTKQGLIVKTRSQSSNNERRNNPEPGDLNDPQEFMAENFELFLSDP
                     EYKCRRPSLARLFQKFFNHIPFPTEHCTQSHQVLLEPDILVDLIPQTIELDLSRLYQI
                     DYLLASKGETISSRWGHSMYRFVFCKPGRPLGPNCRWDTAYHIVASFAAKVDSAEASP
                     LKGLTGAYESRLFFSRFRKVLQDYTKKELRELISLPLNLSSQQKTQFVYRLLEQYWSY
                     KGSYRFLTNNCAHEALSVLQVAMDSRFLAEKTALSPNGLFDLLAETRLVDKQVLTDKI
                     HAEEQSFFVPSFKLRFNDAIKNLKIGVRASFLLEQRIENLSTEPAIIQRRLIFDTIGS
                     SLHGRIGKVSRYHRSLLASLYLIEDYAFTLSQLELSHKMRQKLEEALKAEGLDQMITK
                     LLLPKPIEGLEGGYGIPLASEVESNNDPSQSAIPAELLHRIRLLSEKIQKSHSLEIER
                     LQREDKQKLEILNLLTS"
     gene            2159..2518
                     /locus_tag="VIG33_17450"
     CDS             2159..2518
                     /locus_tag="VIG33_17450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272684.1"
                     /translation="MSQQSWQQIQSQKFQKAITSIESSPYLLHLYFRYCGHPSFVGDE
                     IREIEKLYLQNSEKEWNHFHAKTLNLRLQHLLAQIKVGILWGLTGEINNWKNQEEELA
                     EICTKLFSFFEDLSSAH"
     gene            complement(2536..3213)
                     /locus_tag="VIG33_17455"
     CDS             complement(2536..3213)
                     /locus_tag="VIG33_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272685.1"
                     /translation="MKTFILPLAVFISHGIFSGALPAHAESFRCEGIFAQPVVPKMGY
                     IKNGLCSDNIFYFFRKLKAVHPDEDFSNTQVLYLYPRWTGAYRYNMRTIKFKSGYKLW
                     MFHVVLLHKGLIYEYDHESDNDALPVAQFFEESFGSSWKIQGGHKNYIETVEKTFHND
                     YDDPTPSADSQLRMHVRALPAEVYLNEYSFERDHDMNPQVKNYAHWVYGDPRFPDETL
                     EDFLKKF"
BASE COUNT          995 a          746 c          683 g          895 t
ORIGIN      
        1 ccggtaatta gcttttatta tttttttggg ggttccaatg agattgtttt ttgtgggtct
       61 tatttttata atgactcttt cagcccaagc gcgagaattt tctttaaaga tagaagccaa
      121 ttctatctcc tctctctctc cagaagagcg gaatgcgatt cattcttcgt tggaagccgt
      181 tgaaggcttg ttgccgacaa aaatcaaaga ggccttggcc ccccaagtga cccttcggtt
      241 cccattgttg agttccgaaa aaatcaggga gaaatgggaa cgatggattc gagggatcct
      301 cttgcgagag atttttcgcc tctacgacga caaaaaccac accctgtcgg atcaccccgt
      361 atttttaaca gctgcgggat ggaccaaaca gggtctgatt gttaaaacca ggtctcaaag
      421 cagcaacaac gagcgccgga acaatccgga acctggggac ctcaatgatc cccaagagtt
      481 tatggctgag aactttgagt tgttcctttc agatcctgaa tacaaatgcc gaagacccag
      541 tctggcgcgt ctatttcaga aatttttcaa tcacatacct tttccaacag aacactgcac
      601 tcaatctcat caggtgctgc tagaaccaga tattctcgtc gatctaattc cacaaaccat
      661 cgaacttgat ttgtctcgcc tctatcaaat tgattatttg ctggcttcga aaggtgaaac
      721 catcagctcc cgctggggcc actcaatgta tcgattcgtg ttttgcaagc caggacgccc
      781 gctagggcca aattgccgat gggatactgc ctatcacatt gtcgctagtt ttgcggccaa
      841 agtggattcc gcggaggcca gtcccttgaa aggcctgacg ggagcttatg aatctcgact
      901 ctttttcagc cgttttcgga aggtccttca agattatacg aaaaaagaac tccgagaatt
      961 gataagtttg ccattgaatt tatcatccca acagaaaacg caatttgtat accgactctt
     1021 agagcaatat tggagctaca aaggttccta cagattccta accaacaact gtgcccacga
     1081 ggcgctatcc gtcctgcaag tcgcaatgga ttcacgcttc ctggcggaaa aaacagccct
     1141 ttcacccaat ggtctcttcg acttgttggc tgaaacgaga ttggtggata aacaagtgct
     1201 caccgataaa atccatgcgg aggaacaatc tttctttgtt cccagtttta aattgcgttt
     1261 caacgacgcc atcaaaaacc tcaaaattgg cgtgagagct tcatttttat tggaacaacg
     1321 gattgaaaat ctttcgaccg aacccgccat cattcaacga cgtctcatat ttgacaccat
     1381 tggatcttcc ctgcatggga ggattggtaa agtgagccgt tatcaccgga gccttctggc
     1441 cagcctctac ctcattgagg attacgcctt cactctgagt caacttgaat tatcccataa
     1501 aatgcgccag aagctggagg aagctttaaa ggcagagggt ttggatcaaa tgataaccaa
     1561 gttattactt ccgaaaccca ttgaaggcct tgagggcggt tatggaattc ctctcgcatc
     1621 agaagtggaa tccaataacg acccctctca aagtgcaatt ccagctgaac ttcttcatag
     1681 aatacgtttg ctcagtgaaa aaatccaaaa atcacattcc ctggaaatcg aaagactcca
     1741 gcgcgaagac aaacaaaaat tagaaatatt gaacttatta acctcgtgac caaaacacga
     1801 cccagtacgt ggtcagaaaa agaccttgct caatcccgca aaaaaccatc aaattagcaa
     1861 agcagtgatc gccaggattg gaaaagaacc tagcaaggca gaaatagatg aacactaaaa
     1921 gcaaaagtcc tagaaaaaac taaacttgga gccggtttac ctggaggtgt ccaagttgct
     1981 gttatataaa aatcccgaac aaattagttt ctctttgatc tcaaaaaaaa cgaaggtccc
     2041 gcgaacgaca ttgtattact actttaattc tgacatcaat tctctgatta cggagtctat
     2101 acgtttcacg atgaaagaat ttatgcaaat ctgatcaatc gcatcagaaa attccgcaat
     2161 gtctcagcaa tcgtggcaac aaattcaaag tcaaaaattc cagaaggcaa ttacatcaat
     2221 tgaaagctct ccttacttac tccatctcta ctttagatac tgcggacatc ctagcttcgt
     2281 gggagatgaa attcgtgaaa tcgaaaaact ttatttacaa aattcagaaa aagagtggaa
     2341 tcattttcac gctaaaactc ttaaccttcg cctgcaacat ttgttagcgc aaatcaaagt
     2401 tggaatactt tggggtctca ctggagaaat caataattgg aaaaatcaag aagaagaact
     2461 ggctgagatt tgcaccaaac tgttttcatt ctttgaagac ttatcctcgg ctcattaacc
     2521 ggcgaaagct ggcagttaga atttttttag aaaatcctcc agggtttcat cggggaagcg
     2581 cggatctccg taaacccaat gagcatagtt tttaacttgc ggattcatgt catgatcgcg
     2641 ttcaaatgag tattcattca ggtaaacctc ggccggtagg gccctgacat gcatgcgaag
     2701 ttgagaatcc gcagaaggag tagggtcatc gtagtcatta tggaaggtct tttccacggt
     2761 ctcaatatag tttttatggc ctccctggat tttccagctg cttccgaacg attcttcaaa
     2821 aaattgcgcc actggcaaag cgtcattatc agattcatga tcgtactcgt aaatgagacc
     2881 cttgtgcaga agcacgacat ggaacatcca gagcttgtac ccggatttaa atttaatagt
     2941 tctcatgttg tatctgtagg ctcctgtcca gcgagggtaa agatataaaa cctgagtgtt
     3001 gctgaaatcc tcgtctggat gaacggcctt gagttttctg aaaaagtaaa aaatattatc
     3061 agagcacagg ccattcttga tgtagcccat ctttggaaca acaggctgtg caaaaattcc
     3121 ttcacagcga aaggactctg cgtgcgccgg caaagctccg gagaaaattc cgtgggaaat
     3181 aaaaacagca aggggtaata taaatgtttt catacgcctg tgaattgcaa ggcatatacc
     3241 attgagttaa aaggagacac tcaatcgctc tttttatttt tgggttgctt aatgagacaa
     3301 tttgatcaat tcgttttta
//
LOCUS       DATBTE010000457         9933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_610411, whole genome shotgun sequence.
ACCESSION   DATBTE010000457 DATBTE010000000
VERSION     DATBTE010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 9933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9933
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610411"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VIG33_17460"
     CDS             <1..632
                     /locus_tag="VIG33_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272686.1"
                     /translation="VDHTNKILRNANREHRGERDLITQRNRQDREMLMQQHVDNVGLV
                     ADNAENRVKKITKLSRENQEAYSNYFENSLDQMKDNFTERMVGQREQTTDDMVRLNKA
                     MSERFHGMEKGYNRKVETLTTGYENKINQMKENHEKELKRIENYYAQRLNSKDKEVKQ
                     QTTTLEMKYEAKLAQMNNAHEDQLDRMNRRHQEDMQNLATKVSNYNKKA"
     gene            638..2125
                     /gene="pyk"
                     /locus_tag="VIG33_17465"
     CDS             638..2125
                     /gene="pyk"
                     /locus_tag="VIG33_17465"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY8272687.1"
                     /translation="MLADRRAKIVATIGPSTKDEASLEKAIKAGLNVARLNFSHGTHE
                     DHLKVIHLVRKLSKELQAPVTILQDLQGPKIRVGKFEKGSIFLKKGDQLVITTDNVLG
                     REGLIPSDFKELPQSVQAGTKILLDDGLLELKVLKVRGHDVDVEVAFGGTLKDRKGMN
                     LPGANLPVECMTTKDLEDLEFGLAHGVDYVALSFVRHPRDIRKLRELIESKKSNAKIC
                     AKIEMAEALENLEEISRLSDAIMVARGDLAVEVGQTLLPGIQKKIILLCNQLRKPVIT
                     ATQMLDSMVENPRPTRAEITDVANAVLDGSDALMLSAESASGKNPFLAIRTMHDIIVE
                     VERTEEKYYKISLENEFLSPPAAIAASASLSALKLNATAIVCLTTSGKTANIIASYRP
                     KARIIAVTDRLEVLNTLELTWGIQTLAIKPYKTMEDILDQVDQLLVQYGLAKTGDRVI
                     FTLGQPISDGAKTNALFLHIVGAESQNRTEDENLPLRCQMDPLIS"
     gene            complement(2138..3115)
                     /locus_tag="VIG33_17470"
     CDS             complement(2138..3115)
                     /locus_tag="VIG33_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8272688.1"
                     /translation="MAEGKKVAKDTYLFRDGDAPDAMYIVKSGMLAVTKTKGSSEVII
                     AEIGPGAMVGEMAIFDKKPRSANVKATKDSEVISLPYEGLNQQMDSLPVWVKAIMRTM
                     NENLRESNKKIRLLENPNQDEDRFPPHTIIKLLSLINLVGHKYGKPEEGGLSVPSGTL
                     RRYTIQVFQEATNKMNSMTAALKALGYMTIEDLGEGRQKMINLQSDFLFEFVDWYTDW
                     LFSSDKDKISLKDEEVKIMEGILHFAAKTEPDAKGWHKINLNQLQNDSMKELGRLIRV
                     EDLNPAIEKKLISEKIMAEADIFVTVNPSDVTKITKYWGLINNLKKNLR"
     gene            3292..3870
                     /locus_tag="VIG33_17475"
     CDS             3292..3870
                     /locus_tag="VIG33_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272689.1"
                     /translation="MKPLFLLMTLIISQGQAAFADSFSIIKDGKNYLCTSTNDTPEAS
                     GECIAEAYAGPFFKDEAITLCTGAHSTFPAKCAVKAYAELCTKEESIRLCTGAESMGP
                     VECFNKAYAGAFNKDESLTLCKGNGTVVNADCALKAYAGPYSKKEALRICKNEAQLLM
                     RSLNLMQHSAEIQQKVKLMKLQPSTSAEAPIQ"
     gene            complement(3885..4547)
                     /locus_tag="VIG33_17480"
     CDS             complement(3885..4547)
                     /locus_tag="VIG33_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HEY8272690.1"
                     /translation="MPLNREQIAQRIAQEVQDGDTVNLGIGIPTLVANYIPSTKSVML
                     QSENGLLGMGPFPLEKEVDPDLINAGKQTVTEAPGASYFSSADSFAMIRGGHVDLTVL
                     GAMEVDQNGSIANWMVPGKMVKGMGGAMDLVAGAKNVIVAMQHTDKEGNSKLRTKCTL
                     PLTGVNCIKKIVSDYGVIDVTPNGFVLREYAPDLTPEKVLAATEGKMTVAPDVKPMTF
                     SR"
     gene            complement(4555..5256)
                     /locus_tag="VIG33_17485"
     CDS             complement(4555..5256)
                     /locus_tag="VIG33_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015091186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEY8272691.1"
                     /translation="MSRNKVYEDAKSALEGIKDNMTLVVGGFGLCGIPENSIKALKEL
                     GIKGLTCVSNNAGVDDFGLGVLLHSHQIRKMISSYVGENATFEKQFMSGELEVELCPQ
                     GTLAERLRAGGAGIAGFYTPTGVGTLVAKGKEVKNFDGRDYVLERGIVGDFALVKAWK
                     GDKFGNLIYRKTARNFNPMVATAGKITIAEVEELVEVGSLPPDQIHTPGVYVQRIFQG
                     KGYEKRIEQITVRKA"
     gene            complement(5249..6430)
                     /locus_tag="VIG33_17490"
     CDS             complement(5249..6430)
                     /locus_tag="VIG33_17490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY8272692.1"
                     /translation="MENVVILSAVRTPVASFQGALSTLPAPKLGAFAIKEALVRAKVS
                     PQEIDECIMGEVLTAGVGQAPARQAAIYAGLKNTTPCLTINKVCGSGLKAIMLAADSL
                     QLGHSKIAVAGGQENMSLAPHLLENSRTGYRMGSAAIVDSMVKDGLWDPYNNFHMGSA
                     AEVCVRENHFTREQQDAFAISSYKKAQAAIARGAFKNEISPVVIETKKGSVTIDTDED
                     PANTNFDKIPGLRPAFEKEGTITAANASKLNDGAAAVVLMTESTAQARGLKPLAKIVA
                     QATFAQDPKYFTTAPVGAIKKALQNANLKMGDIDLWEINEAFAVVTQVAMKELEIPAE
                     KVNVHGGAIALGHPLGASGARIFTTLVHALHTYNKRYGLATLCIGGGEAVAVIIEKMH
                     V"
     gene            6535..7539
                     /locus_tag="VIG33_17495"
     CDS             6535..7539
                     /locus_tag="VIG33_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272693.1"
                     /translation="MKRIFSALGFALLILSVFSGHQAHAVLSFEVTPQDIERIVILGL
                     EAQVQLSGQSTAQKLRISGVDETNEPGQFALERKERILFIKMQEYPDKKSWKEALSRV
                     PKRKILELTGVPIPVEIQLREGQVNSQKWNKELKISLIKGKVFSVGGSTSLSIQLQNG
                     EASVQDQTSKLMADVYKGKLQVRNLQGDLDASVFSGSLNIEKSKGFLSINTAQSSAKV
                     LQSSGTLQFENGKGSVMTQQFVGRVDGQTGEGPVSLGILPDTDIHVKSTKGRVSVQTV
                     AGSGAFVNLLSTDGEILVPAELKVNRSAAEKSVRGRLRGGEQKGSIVVRSQEGSIVVK
                     "
     gene            7582..8538
                     /locus_tag="VIG33_17500"
     CDS             7582..8538
                     /locus_tag="VIG33_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272694.1"
                     /translation="MAKMKKKSSPPKNASKKSVAKPIAKLNAAKKSGAKPAGKASTID
                     AKHKKSHPLGKKGKAAEKSPKKTLAPLKEKAKSTVLLKKAVTPAAKVEKKPLEKETKK
                     MAPESLEAKSFIKPAVKPTAKKEGKVVAPKKGAKGKKEETEEEDDFVTDDDFVGTDEI
                     GEYEEELKAVEELDEEVIEDEGWATEIKDRGDEEVFLTDAEGRRYCRARDCDQVGVVD
                     GYCRFHYLLFWRKIQVRKKILADGKLGRYVEELTARYPDKFLEMIRRDLRTEKDFLGA
                     IQELEIDESAGENELEEDASAYIEEVRGIGEGSSAGVDDEEF"
     gene            8823..9290
                     /locus_tag="VIG33_17505"
     CDS             8823..9290
                     /locus_tag="VIG33_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272695.1"
                     /translation="MKNKLIAFMLMASFVLILANCSKSNSTPANSAIASGVCTVPGQI
                     YTTQGCLPQSSACGMNAGYLNGSCYPAAGMSGVNSMCGTGSYGYNNQNYGNTGYTQTN
                     CYPQQGYQQQGTCLSTDVVNTQMGTLTVGVCQPYCAQYGRKYGYSNGSCYPAY"
     gene            complement(9315..>9933)
                     /locus_tag="VIG33_17510"
     CDS             complement(9315..>9933)
                     /locus_tag="VIG33_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272696.1"
                     /translation="RLKTSKKWTSFPKEYVEQIQTVFYENFLEPLAGSKLIIEGRIYP
                     TEVLLRVGINKKGQLRQPNFEVSMDYNPKEKDALERIHNCIDAAASMMADYFELEEEA
                     EFPRTWQEYPFQGKSVFLQFTTENSDLEAQANALLGEDSDALVLEEESNDALAHAEVD
                     PALSDNSEEDFPEEEEEDGENEDSKKEKATGPKMFGGPKKKSQLH"
BASE COUNT         2726 a         2089 c         2393 g         2725 t
ORIGIN      
        1 tggtagatca tacaaataaa attttacgta atgccaaccg tgagcatcgt ggcgaaaggg
       61 acttaataac gcagcgaaat cgtcaagatc gagagatgtt gatgcagcag cacgtagata
      121 atgtgggttt agtggctgat aatgcggaaa atcgcgtcaa gaaaatcacc aagctttcta
      181 gagaaaatca ggaggcctat agcaactact ttgagaactc tttggatcag atgaaagaca
      241 attttacaga gagaatggtt ggccaaaggg agcagacgac agatgacatg gttcgcctca
      301 ataaggccat gagtgagcgt ttccatggca tggaaaaggg ttataatcga aaggtggaaa
      361 ctttaaccac tggttatgaa aataaaatca atcagatgaa ggaaaatcat gaaaaggaac
      421 tcaagcgtat agagaattac tatgctcagc gtttgaacag taaggataaa gaggtaaagc
      481 agcagaccac tactttggaa atgaaatatg aagctaaatt ggcgcagatg aacaatgctc
      541 atgaagatca acttgataga atgaatagac gtcatcaaga agatatgcaa aacttggcta
      601 caaaagtaag taactataac aagaaagcat aatttccatg ttagcagatc gcagagcaaa
      661 aatagttgcc accatcggtc cttctaccaa agatgaagcc tctcttgaaa aagctattaa
      721 ggctgggctc aatgtagcca gattgaactt cagtcatgga actcatgaag atcatctcaa
      781 agtgattcat ctggttcgta aactttcaaa agagcttcaa gctccagtga ctattttaca
      841 ggacttgcaa ggtcctaaaa tccgcgttgg gaaatttgaa aaaggttcta tttttttgaa
      901 aaaaggcgat cagttggtga tcactacgga taatgtatta ggccgtgaag gactcattcc
      961 tagtgatttt aaagaattgc ctcaatccgt ccaggctggc acaaaaattc ttctggatga
     1021 cggtctgcta gagcttaaag tgcttaaagt tcgaggccat gacgtcgatg ttgaggttgc
     1081 ctttggcgga acgctgaagg atcgtaaagg aatgaatttg cccggtgcga atcttcccgt
     1141 ggagtgcatg acaacgaaag atttggaaga tttagagttt ggtcttgccc acggagtcga
     1201 ctatgtggct ctcagtttcg ttcgtcatcc cagggatatt cgtaagcttc gcgaacttat
     1261 tgaaagtaaa aaatccaatg cgaaaatttg cgctaaaatt gaaatggcgg aagctctaga
     1321 gaatctagaa gaaatttctc gtttgagcga cgcgattatg gtggcccgtg gagatctggc
     1381 tgtggaagtc ggacagacac ttttgccagg aatacaaaag aagattattc ttttgtgcaa
     1441 ccagctcagg aagccagtta tcacggcgac gcaaatgttg gacagtatgg tggagaatcc
     1501 tcgaccaacc cgggcggaaa ttacggatgt ggcaaatgcc gttttagatg gctctgacgc
     1561 tttgatgttg tctgcggagt ccgcaagtgg aaaaaatcct tttcttgcga ttcgcactat
     1621 gcacgacatt attgttgaag tggaaagaac cgaagaaaaa tattataaaa tttccttaga
     1681 aaatgaattt ttaagtcctc ccgcagctat tgctgccagt gcgagtcttt cggctttgaa
     1741 actcaatgcc acagctattg tatgcttaac tacttcgggg aagacggcga acatcatcgc
     1801 cagctatcgg cctaaagctc gtattattgc ggttacggat cgtcttgaag tcttgaatac
     1861 tcttgagttg acttggggta ttcagacgtt ggcgattaaa ccttataaaa cgatggaaga
     1921 tattttggat caagtggatc aactcttggt gcagtatgga cttgcaaaga ccggggatcg
     1981 agtgatcttt actctgggtc agcctatttc tgatggagca aaaacgaatg ctttattttt
     2041 acatatcgtt ggggctgaga gtcagaatcg aactgaagat gaaaaccttc ctctccgatg
     2101 tcaaatggat cccttaataa gctagtagcc gcttagttta gcgcaggttc ttttttaggt
     2161 tgttgattag accccaatat ttggtgatct ttgtgacgtc gcttggattc acggtcacga
     2221 agatatcggc ttctgccatg attttttctg agataagttt tttctcgatg gcgggattta
     2281 aatcctctac gcgaatcaag cgtccaagct ctttcatgga atcattttgg agttggttga
     2341 ggttgatttt atgccaacct ttggcatcgg gttccgtttt agccgcaaag tgcagaatcc
     2401 cttccataat tttaacttct tcatctttca aggagatttt atccttgtct gaactaaaga
     2461 gccaatctgt ataccaatcc acaaattcga ataaaaaatc agattgaaga ttgatcattt
     2521 tctgacgtcc ttcaccgaga tcttcgatag tcatatagcc aagagctttc agagctgcgg
     2581 tcatgctatt cattttgttg gtggcctctt ggaaaacttg gatggtgtaa cgacgaagtg
     2641 ttccagaggg gacggaaagt cctccctctt ctggttttcc atatttgtga ccgacgagat
     2701 ttataagtga taaaagttta atgatggtgt gcggaggaaa acgatcttca tcttgattgg
     2761 ggttttccag cagtcgtatt ttcttgttcg actcacgcag gttttcattc attgttctca
     2821 taatagcctt tacccaaaca ggtaaagaat ccatctgctg attgagtcct tcatacggaa
     2881 gcgatatgac ttcagagtcc tttgtcgctt tgacattggc gctgcgaggt tttttatcga
     2941 agatggccat ctctccgacc atcgcgccag ggccaatttc cgcgatgatg acttcagagc
     3001 ttcctttggt tttagtaaca gctagcatcc ctgatttaac aatgtacatc gcatccggag
     3061 cgtctccatc gcggaacaaa taggtatcct ttgcgacttt ttttccctca gccacgaatt
     3121 ttcctctcgc tgttgctcac tttttaatta tgactttaaa gagggggcga gggcaattgc
     3181 cgctgaacat atctctggac ctacctccag tgttacctca ggattccgat gcactttaga
     3241 gttggcatcc tggttacttt atgttagtcg agtccagtcg aaaggggccc aatgaaacca
     3301 ctgtttttat taatgacact tataatttcg caaggacaag cagcctttgc tgattccttc
     3361 tcaatcatca aagatggcaa aaactatctt tgcacctcta ctaatgatac tcccgaggca
     3421 agtggggaat gtattgctga agcctacgcg ggtccttttt ttaaagatga agccataact
     3481 ttgtgcactg gtgctcactc tacgttcccg gcaaagtgtg cggtgaaggc ttatgccgaa
     3541 ctatgtacta aagaagaatc gattcgtctt tgtactggag ccgagtcaat gggccccgtt
     3601 gaatgtttca ataaagctta tgcaggtgca tttaataaag atgaatccct cactctttgt
     3661 aaaggcaatg ggaccgttgt gaatgcggat tgtgccctta aagcatatgc tgggccttac
     3721 tctaagaagg aagctctccg aatttgtaaa aatgaagccc agttgctgat gcgatctctg
     3781 aatctgatgc agcattcagc tgaaatacaa cagaaggtga agttgatgaa gcttcagcct
     3841 tcgacttctg ctgaagcccc cattcaatag ggccagcgca aaatttatct gctgaaagtc
     3901 atcggtttaa catcaggggc aacagtcatt tttccttctg ttgccgcgag aactttttct
     3961 ggggttaaat ccggtgcgta ttcgcgcaga acgaaaccgt tgggagtgac gtcgatgact
     4021 ccgtaatcag agacaatttt cttaatgcaa ttcaccccag tcagcggaag tgtgcacttc
     4081 gtgcgaagtt tggagtttcc ctctttgtct gtgtgttgca tggctacgat gacgttttta
     4141 gctcccgcaa ccaggtccat ggcgccgccc atgcctttga ccattttgcc tggcaccatc
     4201 caattggcga tgctgccatt ttggtctact tccatcgcgc ccagaacagt gaggtccaca
     4261 tgacctccac gaatcatcgc aaaactgtcg gctgagctaa aataacttgc gcccggagct
     4321 tcagtcacag tttgttttcc cgcattgata agatctggat cgacctcttt ttctagagga
     4381 aacggaccca tacccaagag tccattttca ctctgtaaca taacagattt tgtcgatggg
     4441 atataattgg cgaccagggt gggaatgccg ataccgagat taacggtgtc gccatcttga
     4501 acttcctggg caatgcgttg ggcaatttgt tcgcgattta atggcatggg atatttaggc
     4561 ctttctcacg gtgatttgtt cgatacgttt ttcgtagcct tttccctgga aaatgcgctg
     4621 cacatacacg cccggagtgt gaatttgatc gggggggagg cttccaactt caaccaattc
     4681 ttcgacttcc gctatggtga tcttgccagc ggttgcgacc ataggattaa aattgcgagc
     4741 ggtttttcta taaattaaat ttccgaactt atcgcccttc caagctttta caagagcaaa
     4801 gtccccgaca atgccacgtt caagcacgta atcacggccg tcgaagtttt taacttcttt
     4861 gcctttagcc accagcgttc ctactcccgt tggggtgtag aaaccggcga tgccagcgcc
     4921 gccagcgcga agtcgctcgg ccaaggtgcc ctgagggcaa agctccactt caagttctcc
     4981 gctcataaat tgtttctcaa aagtggcatt ttcgcccaca taagaagaaa tcatttttct
     5041 gatttgatga gagtgcaaga gaacgccgag gccaaaatca tcaacgccgg cattattaga
     5101 aacgcaggtg aggcctttaa tgcccagctc tttgagtgct ttaatgctgt tctcgggaat
     5161 tccacaaaga ccaaatccac ccacaaccaa cgtcatgttg tctttgatgc cctcaagagc
     5221 ggattttgca tcctcgtaaa ctttatttct agacatgcat cttctcgatg atcacggcaa
     5281 cagcttcgcc accaccaata cacaaagtgg ctaggccata gcgtttgtta taggtgtgaa
     5341 gggcgtgcac caaggtggtg aaaatgcgag caccactggc gccgagggga tggccaaggg
     5401 cgatggctcc tccatgcacg ttgactttct cagcggggat ttcaagttct ttcatggcaa
     5461 cttgggtgac aacggcgaag gcttcgttga tctcccagag atcaatatca cccattttca
     5521 aattggcatt ttgcaaagct ttcttgatcg caccgaccgg cgccgtcgtg aagtatttag
     5581 ggtcctgagc gaaggtggct tgagccacaa tcttcgccag aggtttaagg cctcgagcct
     5641 gtgccgtgct ttccgtcatg agtacaacgg cggcggcgcc atcgttgagt ttagaagcat
     5701 tggcggcggt gatggttcct tctttttcga aagccggacg aaggccaggg attttatcaa
     5761 aattggtatt cgctgggtct tcatccgtgt cgatggtcac agaacctttt ttggtttcaa
     5821 taaccacggg ggagatttca tttttgaaag cgccccgagc gatggctgcc tgcgctttct
     5881 tataagaaga aatcgcaaag gcgtcttgtt gttctcgtgt aaaatgattt tcacgcacgc
     5941 agacttccgc cgcacttccc atgtggaagt tgttgtaagg atcccaaaga ccatctttga
     6001 ccatggagtc tacgatggca gcggagccca tgcggtagcc agtgcgagag ttttctaaaa
     6061 gatgcggagc caaagacata ttttcctggc cgccggcgac ggcgatttta ctgtgtccta
     6121 attgaaggga gtcggcagcg agcatgatcg ctttaaggcc agagccacaa actttattga
     6181 tagtcaggca aggggtggtg ttttttaagc ctgcatagat agcggcttgg cgagcggggg
     6241 cttgtcctac gcctgcagtc aatacttcac ccataataca ttcatcaatt tcctgaggag
     6301 aaacttttgc acgcacgaga gcttctttga tagcgaaggc gcccagctta ggagcaggta
     6361 aagtgctcaa ggccccttga aaagaagcca ccggtgtacg cacggcgcta agaataacaa
     6421 cgttttccat aaattctcct ttattaaaag aaactcatag tcagagtctg ctcaatcttt
     6481 tatattttga agacagagtc aaatggacct tcaaagatta aacggagtga cagaatgaaa
     6541 aggatttttt ctgccctggg tttcgcttta ctcattttga gtgttttttc agggcatcaa
     6601 gctcatgcag ttctcagttt tgaagtaact cctcaagaca ttgagcgaat tgtgattctc
     6661 ggcttagagg ctcaagtgca actcagtgga caatcgacgg ctcaaaagct gcgcatttcc
     6721 ggcgtcgacg agaccaatga gcctggacaa tttgccttgg aaagaaaaga acggatcttg
     6781 tttataaaaa tgcaagagta tcctgacaaa aaaagttgga aggaggctct ttccagagtt
     6841 ccgaaaagaa aaattcttga actcactgga gttcccattc ccgtggaaat tcaactccgc
     6901 gaagggcaag taaatagcca aaaatggaat aaggaactta agatctcctt aattaaaggg
     6961 aaagttttca gcgtgggagg cagcacttcc ttgagtattc agctgcaaaa tggtgaagct
     7021 tcagttcaag atcaaacttc aaagttgatg gcggatgttt acaaaggaaa actgcaagtt
     7081 cgcaaccttc agggagacct ggatgccagc gtgttttcgg gatctctgaa tatcgaaaaa
     7141 tcaaaaggtt ttttatcaat caatacggct cagtcttccg ccaaagtttt gcagagttct
     7201 ggcactttgc aatttgaaaa tggcaaagga agtgtgatga ctcagcagtt tgttggccgt
     7261 gtcgatgggc aaacgggcga ggggccggtc agcctgggta ttttgcctga cacggatatt
     7321 catgtaaaaa gcaccaaggg gcgtgtctcg gtgcaaactg tggcgggctc gggggctttt
     7381 gtaaatttgt tgagtaccga cggggagatc cttgttcctg ctgaacttaa agtgaaccga
     7441 agtgccgccg aaaaatccgt acgggggcgt cttcgcgggg gtgagcaaaa aggcagtatc
     7501 gttgtgcgtt ctcaagaggg aagcattgtt gttaaataat cagttgacag taactttgat
     7561 atcattcaag gctctattca tatggcaaaa atgaaaaaaa aatcaagtcc ccctaaaaat
     7621 gcttcgaaaa aatcagtagc taaacctata gctaaactta atgcagcaaa aaagtcagga
     7681 gctaagcccg ctggcaaagc ttctactatt gatgcaaagc ataaaaagtc tcaccctctg
     7741 ggaaaaaaag gcaaagctgc tgaaaagtct ccaaagaaaa ccctggctcc acttaaggaa
     7801 aaagccaaat cgacggttct tcttaagaaa gccgtgacac ctgctgcgaa ggttgagaaa
     7861 aagcccctcg aaaaagagac aaagaaaatg gctccagaat ctctagaggc caaatcattc
     7921 attaaaccag cggtgaagcc gacggctaaa aaagaaggca aagtagtggc tcctaaaaaa
     7981 ggtgctaagg gcaaaaagga agagacggaa gaggaagatg attttgtgac cgatgatgac
     8041 tttgtaggca ctgacgaaat cggtgaatac gaggaagaac tcaaggccgt tgaagagttg
     8101 gatgaagaag tcattgaaga tgaaggttgg gccactgaaa tcaaagatcg tggtgatgag
     8161 gaagtcttcc taacggatgc cgaaggccgc cgttattgtc gcgcgcgaga ttgtgatcaa
     8221 gtgggtgtag tggatggtta ttgccgattc cattacctct tgttttggag aaaaatccaa
     8281 gttcgtaaga aaattttagc tgatggtaaa ttgggaagat acgttgaaga actgacagct
     8341 cgttatccag ataaattctt ggaaatgatc cgtcgcgatt tgcgtactga aaaagatttc
     8401 ctcggagcta ttcaagagct tgagattgac gagagtgccg gagaaaatga acttgaagaa
     8461 gatgcaagcg cgtatataga agaagtccgc ggcatcggcg aaggcagcag cgccggcgtg
     8521 gacgacgaag agttctagtc aaaggttgta agaagtaaga gttttaagcc taaatttgaa
     8581 gttttgaagt gggacttgga agtcccattt tttttctttc tcagaggccc atagctatgc
     8641 cagaaaacac atgcacgaga tgttgcttat ccctttaagt gttagcaatt tacggcgctg
     8701 acattgcctg tcctaatttg aggcgctcca aaccttttat attcgttgcg accgggccag
     8761 cttgtgcacg cttcttgtag tacctctggg taatgagatt aattttaaca aagaggtagt
     8821 agatgaaaaa caaacttata gcatttatgc tgatggcgtc ctttgtctta atcttggcga
     8881 attgtagtaa gagcaatagt actcccgcga atagtgcgat tgcaagtggg gtctgcacgg
     8941 tgccaggcca gatttacacc actcaaggct gtttgccgca atcctcggct tgtggaatga
     9001 atgcaggtta tttaaatgga tcttgttatc ctgcggcagg aatgtctggt gtcaattcaa
     9061 tgtgcggtac tgggagctat ggatataata accagaacta cggaaatacc ggatacactc
     9121 agacgaattg ttacccacag caaggctatc aacaacaagg aacctgttta tcgacggatg
     9181 tcgtaaacac tcagatgggg acactcactg tcggagtctg tcagccttat tgtgcccaat
     9241 atgggagaaa atatggctat tcaaatggtt cgtgctaccc ggcatattaa gaactgacga
     9301 aactcctttt tttgttagtg aagctgactc tttttcttag ggccaccgaa catttttgga
     9361 cccgtggctt tttctttttt agagtcttca ttctcgccgt cctcctcctc ctcttccgga
     9421 aagtcttctt cggagttatc agagagagca ggatcaactt cagcgtgagc taaagcgtcg
     9481 ttgctttctt cttcaagaac cagtgcgtcg ctgtcttcac ctagtaaagc gttggcttga
     9541 gcctcgaggt cagagttttc tgttgtgaat tgaaggaaga cgcttttccc ttggaaagga
     9601 tattcctgcc aagttctggg gaactcggcc tcctcttcaa gttcgaaata atcagccatc
     9661 atagaggccg ctgcgtcaat gcagttgtgg atgcgttcca aggcgtcttt ttcttttgga
     9721 ttgtaatcca tggaaacttc aaaattaggt tgacggagtt ggcctttttt attgattccg
     9781 acgcggagca aaacttcagt ggggtaaata cggccttcga tgatcagctt actcccagct
     9841 aatggctcta aaaaattttc gtaaaagacg gtttgaattt gttctacgta ttccttaggg
     9901 aagctcgtcc atttttttga agttttaaga cgt
//
LOCUS       DATBTE010000458         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_541252, whole genome shotgun sequence.
ACCESSION   DATBTE010000458 DATBTE010000000
VERSION     DATBTE010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541252"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1229
                     /gene="mnmH"
                     /locus_tag="VIG33_17515"
     CDS             135..1229
                     /gene="mnmH"
                     /locus_tag="VIG33_17515"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016785 - selenotransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEY8272697.1"
                     /translation="MSHPTIPATEAFRLLSANNQATVIDVRSEGEYEKAHFENTVNIP
                     ILSDAHRHDVGLTYKTEGSEAAKVLGHKLVSGDYKERMIQQWCASIKSHPQQPALIFC
                     WRGGLRSRLAQDWVCQNGLEVFRVDSGYKGLRHEALKAIENPAPFIVLSGMTGSGKTR
                     LIHRLRHFVDLEALANHRGSAFGAYVGRSQPQQATFENKLAQALYQVADNFVLEDESP
                     NIGRCHVPDLFYARMASTAMVMIETPARVRALEIFKEYIQAPFAGGMPSHELEASFAK
                     NVERIRNKLGGLECDNIKTMLSQSFQFDALNEAYADAYLDWIERLLTHYYDKLYIHSL
                     SLKSRETLFQGSWQDCLDFLVDSQERTSHQ"
BASE COUNT          438 a          400 c          384 g          412 t
ORIGIN      
        1 tcgggaagtt atttttaacc aaatccttaa caaccacctc atctgacaaa tcaacgctct
       61 ctttcagcgt agcaagaaga tcgccagccg tttctgttat attaagacct actttactgc
      121 ccctgatttc atccatgagc caccctacca ttcccgctac cgaggctttt agactgctta
      181 gcgccaataa tcaagcgact gtgattgatg taaggtcgga aggggaatat gaaaaggcgc
      241 acttcgaaaa cacggtaaac atcccgatac tttcagatgc ccatcgccat gatgtggggc
      301 tgacctataa gaccgaaggc tcggaagccg ccaaggtgct tgggcacaaa ctggtcagcg
      361 gcgattataa ggagcggatg attcagcaat ggtgcgcatc gattaaaagc catccccagc
      421 aacccgcgtt gatcttttgt tggcgcggcg gcctgcgctc ccggttagcc caggattggg
      481 tctgtcaaaa cggacttgaa gttttccggg tcgattccgg ctataaaggt ttaagacatg
      541 aggcacttaa ggccatagaa aatcccgctc cctttatcgt cctgtccggc atgaccggct
      601 cggggaaaac ccgcttaatc catcggctcc ggcactttgt cgatcttgaa gcgcttgcca
      661 atcatcgcgg cagcgctttc ggagcctatg ttggcagaag ccagccccag caagccacgt
      721 ttgaaaacaa gttggcgcaa gcgttatatc aagttgccga taactttgtg ctggaagatg
      781 aaagtcccaa tatcggccga tgccatgtac ctgatttgtt ttatgcccga atggcctcga
      841 cggcgatggt gatgattgaa acaccggcca gggttcgggc attggaaatc tttaaggaat
      901 atatccaagc gccttttgca ggcggcatgc cgtcacacga gctggaagca agttttgcca
      961 aaaatgtcga acgtatcagg aacaagctgg gcggcctgga gtgcgataat attaaaacca
     1021 tgctgagcca atcgtttcaa ttcgatgcgc tgaatgaggc ttacgcagac gcctatctcg
     1081 attggatcga gcgcctgctg acgcactatt acgacaaact ttatatccat tcgctgtcat
     1141 taaaatcacg agaaaccttg tttcaaggct cctggcagga ctgtctggat tttttagtcg
     1201 attcccaaga aagaacttcc catcaataga tcgatctgta aaaaataggg tccacgaaaa
     1261 acacgaaagg cacgaaaaaa gcagtcgaaa gaatcttgtt gcaagatttt tcgtaactat
     1321 tcagcgtatc ttgcgctggt tcccaagctc cggcttggga acccagttag cgaagctcca
     1381 gcttcgcgag tcgggaagct ggagcttcca agactgcatt cccaagccgg agcttgggaa
     1441 tgagcgaaat ttactcaatt accatgaaga gcatgaagga catgaagact tcaatctttc
     1501 atgttcttca tggtttaaac ataaaacttg ctcaagttat ttcgtgcttt tcgtgccttt
     1561 cgtggacaat gctttttgct agattcatta tcggaaatcc tttgacttat aaataaaaac
     1621 tcaaccccga tagt
//
LOCUS       DATBTE010000459         8037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_884441, whole genome shotgun sequence.
ACCESSION   DATBTE010000459 DATBTE010000000
VERSION     DATBTE010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884441"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /gene="gatA"
                     /locus_tag="VIG33_17520"
     CDS             <1..1182
                     /gene="gatA"
                     /locus_tag="VIG33_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEY8272698.1"
                     /translation="NFVPPYDATAVDRLKKAGVVIIGKLNQDEFAMGSSNETSFYGNV
                     KNPWNLECVPGGSSGGSAAAQAARLCAGTLGTDTGGSIRQPASFCGIVGVKPTYGRVS
                     RYGIIAYASSLDQAGPMVSTVKDAALTMEVISGHDERDSTTSQRKVPHWSKELSANIK
                     GMKIGLIKEYMQGGLHPDVQKTVDQSIAAVKSLGAEVIEVSVPMTEFAVPVYYLIAAS
                     EASSNLARYDGVKYGYRAEFANLSVIDLEDFYGKTRGQGFGKEVKRRIMLGTYCLSSG
                     YYDAYYNKAGQVRRMIMNQFKEAFKKCDVILSPVTTSPAFKIAERISDPLTMYLNDIY
                     TTSTNLAGLPGMSVPFGISTEGLPVGIQLTAPHFEEQKMLNVGYALEGASLVKGKTPN
                     V"
     gene            1175..2608
                     /gene="gatB"
                     /locus_tag="VIG33_17525"
     CDS             1175..2608
                     /gene="gatB"
                     /locus_tag="VIG33_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEY8272699.1"
                     /translation="MYRGYEAVIGIEIHVQLNTKSKMFCADSTQFNAGDNENTSPVSV
                     GMPGTLPVVNKKAIEFAVKTGLALGCEIRKKSVFSRKNYFYPDLPKGYQISQYDQPIC
                     EHGTITFKVGDQTKTVSITRAHLEEDAGKSIHQGEFTMINYNRAGIPLLEIVTGPDMR
                     TPQEAAEYGRTIRKIVRYLGVCDGNLEEGSMRCDCNVSVRKTGAEKFGTRTELKNINS
                     FRFVEKAIEYEIERQIDMVERGEKIVQETRLWDPDKNKTFTMRTKEDAHDYRYFPDPD
                     LKPLIVTEELINKMKSELPELPIAKKARFVSTQGLPEYDAEVLTMEKDLADFYEETAK
                     VCNNYKAASNWIMTELLRELNNNNLEIKVSPIKPIQLGQLIALIDKGTISGKIAKAVF
                     LDMWKTSKDPEVIVKEKGLVQVSDPAVVEKIVDEVLAANAQQVEDHKSGKKKNLFGFF
                     VGAVLKASKGQANPEIVNEILQKKLNG"
     gene            2611..3522
                     /locus_tag="VIG33_17530"
     CDS             2611..3522
                     /locus_tag="VIG33_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEY8272700.1"
                     /translation="MRVAALDLGTNSFLCLIVEGDKAGIKEVLSDQVKVVRLGQGVDK
                     TGSFHPDALARAKACLKEFKQEIDKHNVDRILAMATSAARDAKNGQELFKIGLDLNIP
                     IEIIPGRDEAQITFAGATEGVFQGDENIIVIDVGGGSTEFIVGNKSKLHFSKSLNLGC
                     VRMTEKYITTQPISQKERVIVEEAISQQLEAILPEVQKYAAKEILAVAGTPSALAIAE
                     IGKFDSEKVQGFVLDQNKLKKWCDILASTSVQEKIDKYKIETGRADVLFVGATILHHF
                     LLKMNRPSMRVSIKGVRYGVALEALRR"
     gene            3527..3946
                     /locus_tag="VIG33_17535"
     CDS             3527..3946
                     /locus_tag="VIG33_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEY8272701.1"
                     /translation="MLFFLGTTAHPTDITFEKGKITLGGKTILVEIAKNHTQYERGLM
                     FRKNLPENEGMLFIFDEEEIRYFWMKNTYIDLSIGYFDKKMGLVDIQEMKAPSVLESR
                     PPSYPSAKPAMYALEMKKGWFTKNKIKIGQKLRISSR"
     gene            4077..5387
                     /locus_tag="VIG33_17540"
     CDS             4077..5387
                     /locus_tag="VIG33_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEY8272702.1"
                     /translation="MFKKFVVLLTCIGVFQLSSCTSKDSKEDGGEEMAIDATATDSAE
                     VEKVEGEQSLDIAAEDPSSASSLSTDALPEEALGESSTANQPSKSTPPTPEVAGSEPP
                     PLETLPPDTTTLPPDTLSDSTAEIAPPVTETVEPTHETASSSKSSSEEPKADPKPVAA
                     YRKVEQAPWKEGGKLLNTVYVARNGDSWGSVSQMIYGTERVSELKKMNPSIKGRKLKV
                     GDKVYYNSPHRPDDSVKVLTFYEDSGIAPEVYIAKSGDNLKKVAKELFGSEQGWKELY
                     ATNDFESKAALDEGTQIKYWRTVPAEPKEVAQAQPTMPTPPTSLPPAPPQMNEAPPPP
                     APSEPIAEMPPPPPPPDMAANEMAPPPPPPPPPVEAVAPPAAPPKDMAKQNKVDDAAS
                     PLEDDQMTTLAAGAVVAVGLALFMIMRRRRKKELEQAIQDTQVG"
     gene            complement(5453..5818)
                     /locus_tag="VIG33_17545"
     CDS             complement(5453..5818)
                     /locus_tag="VIG33_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY8272703.1"
                     /translation="MDTNKHPAPRTPLFLEVGFKRNYAREDVKGILKNISLSGAFLEF
                     QGDSPKANEKLYLRFVVSGRERKIAAAVVWGNSHGCGIRFMPANNRDVQIVDDLIYYV
                     ENSREGRRDVLDKIFKKVS"
     gene            6847..>8037
                     /gene="rho"
                     /locus_tag="VIG33_17550"
     CDS             6847..>8037
                     /gene="rho"
                     /locus_tag="VIG33_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY8272704.1"
                     /translation="MKSKNIQQLTELALKLKIENAAGMRRQDMIFEILKRAARLGVDI
                     YGSGVLEILPDGYGFLRSPDYNYLPGPDDIYVSPSQIRRFGLRTGDTVTGTVRPPKEG
                     ERYFALLKVDSLNFETTEKGKDKILFDNLTPLYPNERLKLEHNPSDYTTRVVDLMAPL
                     GKGQRALIVAPPRTGKTVLMQNIANAITTNHPEVKLIVLLIDERPEEVTDMQRTVKGE
                     VISSTFDEPPTRHVQVAEMVIEKAKRLVEHKHDVVILLDSITRLARAYNTVVPPSGKI
                     LSGGVDSNALHKPKRFFGAARNIEEGGSLTIIATALVDTGSRMDEVIFEEFKGTGNSE
                     IHLDRKLMEKRIFPCMDINKSGTRKEDLLIDKGELNRLFILRRVLAPMNVVDAMEFLI
                     DKVQG"
BASE COUNT         2424 a         1656 c         1840 g         2117 t
ORIGIN      
        1 aattttgtgc ccccgtatga cgccactgct gttgatcgtt taaaaaaagc gggagtggtc
       61 atcattggaa aattgaatca ggatgaattt gccatggggt cttcaaatga aacttcattt
      121 tatggaaatg ttaagaatcc atggaacctt gagtgtgttc ctggaggatc ttctggcggt
      181 tctgcggcag cccaagccgc tcgtttgtgt gctggcactc ttgggacaga taccggcggc
      241 tccattcgtc agcctgcaag cttttgcgga attgtcggag ttaagcccac ttatgggcga
      301 gtgagccgct atggaattat agcctatgcc tcttctttgg atcaagccgg gccaatggta
      361 agtacggtta aagacgcagc ccttacaatg gaagtcatca gcggccatga tgaaagagat
      421 tccacaacaa gccaacggaa agttcctcat tggagcaaag agctctcagc gaacattaaa
      481 ggcatgaaga tcggtcttat taaagaatac atgcaaggtg gtttgcatcc ggatgtgcaa
      541 aaaacggtag atcaatccat agcagctgtg aaatcattag gtgcagaagt tattgaggtc
      601 tcagttccca tgactgagtt tgcggttcca gtctattacc ttattgcagc aagtgaagcg
      661 tctagtaact tagctcgcta tgatggagtg aagtacggat atcgcgcaga gtttgccaat
      721 ctttcagtga ttgatcttga ggatttctat ggaaaaactc gcggtcaggg gtttggcaag
      781 gaagtaaaac gcagaatcat gcttgggacc tattgccttt caagtggata ttatgatgct
      841 tactacaata aagctgggca agttcgccgc atgattatga atcaatttaa ggaagccttc
      901 aaaaaatgtg atgtgatttt gagtcctgtg accacgtccc ctgctttcaa aatcgctgaa
      961 cgcatttctg atcctctaac aatgtacctg aatgatattt atacaacctc aactaattta
     1021 gctggtcttc cgggcatgag tgtgcctttt ggaatctcaa ccgagggcct tcccgtagga
     1081 atccagctaa cggcgccgca ctttgaagaa cagaagatgt tgaatgtagg ctatgcgctt
     1141 gagggcgcct ctttggtgaa aggaaaaact ccaaatgtat agagggtatg aggccgttat
     1201 cggcatcgaa attcacgtgc aactaaacac caaaagcaaa atgttctgtg cggattccac
     1261 tcaattcaat gccggagata atgaaaacac ctctcctgtc agtgtcggca tgccggggac
     1321 tcttcctgtg gtaaacaaaa aagccattga gtttgcggtc aagactggct tggcactagg
     1381 atgtgaaatt cgaaagaaat cagtgttctc tcgaaaaaat tatttttacc cggatttacc
     1441 caaaggctat caaatttctc aatacgatca gccgatctgt gaacacggta cgatcacttt
     1501 taaagtggga gatcaaacta agaccgtcag tatcactcga gctcatttag aggaagatgc
     1561 gggtaagtcg attcaccagg gggagtttac catgatcaac tacaatcgtg ctggtattcc
     1621 ccttttagag attgttactg gtcctgatat gagaactccg caagaggccg cagaatacgg
     1681 gagaacgatt cgtaaaattg ttcgttatct tggagtctgt gatggaaact tagaagaagg
     1741 ctctatgcga tgcgattgca atgtgagcgt tcgtaaaaca ggggcagaaa agttcggcac
     1801 acggactgag ttgaagaata tcaattcctt ccgttttgtt gaaaaagcaa ttgagtatga
     1861 gattgaacgc caaattgata tggtagaaag aggtgaaaag atcgtccaag agactcgcct
     1921 ctgggatcct gataaaaaca aaactttcac gatgcggact aaggaagatg ctcacgacta
     1981 tcgttacttt ccagatccgg atttaaagcc gcttattgtg actgaggaac ttattaataa
     2041 aatgaaatct gagcttcctg agctgccgat tgcgaaaaaa gcgcgatttg tttctacaca
     2101 aggccttccc gaatatgatg cggaagtttt gacaatggaa aaagatcttg ccgattttta
     2161 cgaagaaact gcaaaagttt gcaataacta taaggctgcc tcgaactgga ttatgacgga
     2221 acttttacga gagctaaata ataacaattt agaaattaaa gtttctccaa tcaaaccgat
     2281 acagttgggc caattgatcg ccttgattga taaaggaaca atctcgggaa aaattgcgaa
     2341 agctgttttt ttggacatgt ggaaaactag taaagatccc gaagttattg taaaagaaaa
     2401 aggtcttgtt caggtttccg atcctgcagt ggttgaaaaa attgttgatg aagtcttagc
     2461 ggcgaacgct caacaagtag aagaccacaa atcaggaaag aaaaagaatc ttttcgggtt
     2521 ttttgtaggg gcggttttaa aggcctctaa gggtcaggca aaccctgaga ttgtaaatga
     2581 aattcttcaa aagaaactaa acggttagtt atgagagtcg ccgcccttga tctcggaaca
     2641 aactcattcc tttgtttaat tgtcgagggc gataaagccg gcattaaaga agtcctctcc
     2701 gatcaagtca aagttgtacg tttaggtcaa ggagttgata agaccggctc tttccatccc
     2761 gatgctttgg cacgagccaa agcatgcctt aaggagttta aacaagaaat tgataaacat
     2821 aatgtggatc gaatccttgc gatggccacc tctgctgccc gcgatgctaa aaatggtcag
     2881 gaacttttta aaatcggcct agatcttaat attcccattg aaattattcc aggccgtgat
     2941 gaagcgcaaa tcacctttgc tggagctacc gaaggtgtgt ttcaaggaga cgagaacatc
     3001 attgtcattg atgttggtgg cggttcaact gagtttatag tcggtaataa atcaaaactg
     3061 catttttcaa aaagtcttaa tctgggttgt gttcgaatga cagaaaaata tattactaca
     3121 caacccattt ctcaaaaaga gcgtgtcatt gtggaagaag ccatatctca gcaacttgaa
     3181 gccattttgc ctgaggttca gaaatatgcg gcaaaagaga ttcttgcagt tgcgggaacc
     3241 ccctccgctc ttgcaattgc cgagattgga aagtttgact ctgagaaggt gcagggattt
     3301 gttttagatc aaaataaact caaaaaatgg tgcgatattt tagctagcac ctcagttcaa
     3361 gaaaaaatag ataagtataa aatcgaaaca ggtcgcgcgg atgttttgtt tgttggtgcc
     3421 actattcttc atcatttttt acttaagatg aatcggccct ctatgagagt atccatcaaa
     3481 ggagttcgct atggtgtcgc gttggaagct cttcgccgtt agtattttgt tatttttttt
     3541 aggaactacc gctcacccga cagatatcac ctttgaaaaa ggaaagatta ctcttggggg
     3601 aaaaactatc ctggtagaga tagcaaaaaa ccacactcaa tatgaacgtg gtttaatgtt
     3661 taggaaaaat cttcctgaaa atgagggaat gctttttatt tttgatgaag aagagattcg
     3721 ctacttttgg atgaaaaata cttatataga cttaagtatt ggttattttg acaaaaaaat
     3781 gggccttgtc gacattcagg agatgaaagc gccgagtgtg ttggagagta ggcctccctc
     3841 ctatcctagt gccaagcctg caatgtacgc gttggaaatg aaaaaaggtt ggtttacaaa
     3901 aaacaaaatt aagattggac agaagcttcg aatttcaagt cgctgacaat agtctagacg
     3961 gcatcggcct ttaaccaggc taggtcataa aatatttgca aggcatcaat cttatgatta
     4021 aatcattata gattattgaa acagaatgtt tcagcgcatt aatcaaggat gaatatatgt
     4081 ttaaaaagtt tgttgtactt ctcacgtgta taggggtttt tcaactctct agttgtacgt
     4141 caaaagattc caaagaagac ggtggcgaag aaatggccat tgacgcaacg gccactgatt
     4201 ctgccgaagt tgaaaaggtg gaaggtgaac aaagccttga tatcgcagca gaggatccct
     4261 cttcggcttc gtcgctttct accgatgcac ttcctgaaga ggctctcggt gagtcctcaa
     4321 cggcgaatca gccttcgaag agcaccccgc caactccgga agttgcaggg agtgagcctc
     4381 cacctctaga aactttgcca ccagacacta caactcttcc gcctgatact ttgagtgatt
     4441 ccactgcgga aatagctcct ccggtaacag agactgtaga acctactcat gaaaccgcct
     4501 cttcttcaaa atcctctagt gaagagccca aagcggatcc taaacccgtg gctgcttatc
     4561 gtaaggttga gcaagctcca tggaaagaag gaggcaagct tctcaacacc gtatatgtag
     4621 ctcgcaatgg ggattcttgg ggaagtgtta gccaaatgat ttatggtacc gagagagttt
     4681 ccgagttaaa gaagatgaat ccttcaatca aagggcgtaa actaaaagtc ggagataagg
     4741 tttattataa ttcaccccat cgtcctgacg acagtgtgaa ggttcttact ttttatgaag
     4801 acagtggaat cgctcccgaa gtttatattg caaaaagcgg tgataactta aaaaaagtag
     4861 cgaaagaact tttcgggagt gagcaaggtt ggaaagagct ttatgcaacc aatgactttg
     4921 agtctaaagc cgctcttgat gaagggacac aaattaaata ctggagaaca gttccagcgg
     4981 agccaaaaga agtggctcaa gcgcagccta caatgccaac accaccaacc tctcttcctc
     5041 cggctcctcc gcagatgaat gaagccccac caccaccggc accatcagag ccgattgcag
     5101 agatgccacc cccaccgcca cctccagaca tggcagcgaa cgagatggct ccgccgcccc
     5161 ctccaccccc tccaccggtt gaagctgtag ctcctccagc agcgccacca aaagatatgg
     5221 caaaacaaaa taaagttgat gacgccgcaa gtcctctgga ggatgatcag atgacaactc
     5281 tggctgccgg tgcagtggtt gcagtgggtc tcgcactttt tatgatcatg agaaggcgtc
     5341 gtaaaaaaga attagaacaa gctattcagg atacccaagt aggctaagaa aagacaataa
     5401 aagacaaggg gttaaaagac cccctgtctt ttagaatata aattactgac cactaagaga
     5461 cttttttgaa tattttatcg agaacatcac gacggccttc acggctattt tctacatagt
     5521 aaataagatc atcaacgatt tgcacatcgc gattgtttgc aggcataaat ctaatgccgc
     5581 acccatgtga gttaccccag accacggcag cggcaatttt gcgctcgcgc ccactcacca
     5641 caaatcgtaa ataaagtttt tcgttggctt tggggctgtc tccttgaaac tcaaggaagg
     5701 ctcctgagag gctaatgttt tttaagatac ctttcacgtc ttcacgtgca tagtttctct
     5761 taaatccgac ttccaaaaag aggggagtgc gcggagcggg atgcttgttt gtgtccacat
     5821 ttatctcctt tgataattcc taggctaagt gtgtgtattt tatatcggtt tctgatctaa
     5881 aaaactgaag ccttgttgaa taagtactaa tttgagagcc tgaacgtttc attatgaata
     5941 ccaatgagtg gcgtatctat ttgatactac ttaataaaat aaaaacaaaa aagaaatgaa
     6001 gggtttcttt tgaaaatatg cagacatttt caaatcatcg atttttcgtt gacaaaactg
     6061 gactgcttca gcaaattaag agagaacttg agcagacgac cttctcatca ttcagagact
     6121 aggtcccatt taaatctaaa ccagtgtctc taaacctaaa taattcttaa aaaccagctt
     6181 aattaaatat aaaaatacct gagagtcgat actaaggagt aaagtttgtc agatccaaaa
     6241 aatacgcaaa acgttgaggt tgctaaaaaa cgtcccactc gagctaaggc taagacagaa
     6301 gcaactgaag ctcctgtagt cgatgttgtc gccgacgcgc aaccgcctcc accgatggaa
     6361 gctcgcaagt caacacccac tcctctggac ccccctcctc agcaaaatcc tgaaccctct
     6421 tcgcaaggga atcagtcggt tccgcctccc cctccggcaa tttcccctca atcggaaccg
     6481 aatcaaaaag ttcaagaaaa tcgtaatagt ggtgatcgta gcgagcgcaa tgagcgggag
     6541 aatcgcaatg gcgatcgtgg cggagatagg ggggacaatc gcgggggaga tcgtaacggt
     6601 ggatatcgcc gtttcgataa taaaaatcgc gataaccgtg gtggtggcca gcaaaggtat
     6661 caacaaggcg gtggtggccc ccgaagagat aacttccaaa aatacgaaaa tagagatcgc
     6721 aatcaaaatc gttcacaaaa tgaagaaatg ggtgttgagg accagcaatc aagtcaaaat
     6781 cttgatgtag aacttgccga tatcaatttg agcgatgagg aaaaaagctg gctcaactct
     6841 aaagatttga aatctaaaaa tatccaacag ctcacagaac tggctcttaa attaaaaatt
     6901 gaaaatgctg cgggaatgcg ccgtcaagat atgatcttcg aaatcctcaa gcgagcagct
     6961 cgcctgggtg tggatatcta cggctctgga gttcttgaaa tcttgccaga cggttatggt
     7021 ttcttgcgtt cgccagatta taattatctt cccggtccag acgatatcta tgtaagccct
     7081 tcgcaaattc gtcgcttcgg tttaagaact ggcgacacgg tgaccggaac ggttcgtccg
     7141 cctaaagagg gtgaaagata ctttgcactt ttaaaagtag actctttgaa tttcgagact
     7201 accgaaaagg gtaaagataa aattctcttt gataacttaa ccccgcttta tcctaatgag
     7261 cgtttgaagc ttgaacataa tccaagcgat tataccaccc gcgtggtgga cttgatggct
     7321 ccgcttggaa aaggccagcg tgccttgatc gtagcgccgc caaggaccgg taaaacagtt
     7381 cttatgcaaa atatcgccaa tgctattacc accaatcacc ctgaagtgaa gttgatcgtt
     7441 cttttgatcg atgagcgtcc ggaagaggtg accgacatgc aaagaacagt gaaaggtgaa
     7501 gtgatttcat ctacttttga tgaaccacca actcgtcacg ttcaggttgc agagatggtc
     7561 attgagaagg caaagcgtct tgtggaacac aaacatgatg tggtgattct cttggattct
     7621 attactcgct tggcccgcgc ttataataca gtggttccac catcgggaaa aatcttgtcc
     7681 ggcggtgtgg attcaaatgc ccttcacaaa ccaaagcgtt tcttcggtgc ggctcgaaat
     7741 atcgaagaag gtggatctct taccattata gcgacggctc tggttgatac aggttctcgt
     7801 atggacgagg ttattttcga ggagtttaaa ggaacgggta attccgaaat tcatttagat
     7861 cgtaagctta tggaaaaacg tatttttcct tgtatggata ttaataagtc cggaactcgc
     7921 aaagaagact tacttattga taagggtgag ttgaatcgcc tctttatttt aagaagagtt
     7981 ttggcgccca tgaatgttgt agatgccatg gagtttttga ttgataaagt tcaaggt
//
LOCUS       DATBTE010000460         7132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_950791, whole genome shotgun sequence.
ACCESSION   DATBTE010000460 DATBTE010000000
VERSION     DATBTE010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 7132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7132
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950791"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VIG33_17555"
     CDS             complement(<1..375)
                     /locus_tag="VIG33_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272705.1"
                     /translation="MGNTLLKRTLAMVLVLGSLNAWSREVTESNPALSAELNSTMNTM
                     NQQKATLALESEVSTLDNEEYQAAEKQTVMEINRLSSDIKDLERQQRSLNQEADRSKI
                     KAELASKRLQLKKTQQADAQILL"
     gene            548..1000
                     /locus_tag="VIG33_17560"
     CDS             548..1000
                     /locus_tag="VIG33_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272706.1"
                     /translation="MTETLIYGFLFVLGFCLIIFRPIERSIGVSPYWEIGFGSLLVIF
                     FAIFITSSFILEPMFEEDLAKKPCGGSSPQGNCYSLEHSLCETAWQGAESQCKAEMDL
                     ILKARPSALIGPTMNRCRAKRMDQALRFNRINTHTSYCKAYFEYIDRK"
     gene            1004..1549
                     /locus_tag="VIG33_17565"
     CDS             1004..1549
                     /locus_tag="VIG33_17565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272707.1"
                     /translation="MAELIENIFMDSFKREFDLGSMTAEAQDLVKTMSKQQNADFASS
                     SGGHFSAESREFIAKESAFRLVKALSSKNATEVDRDLLQQEWNKIVRDFYQLNFWGQV
                     PQKSKPAKVKTEEQKRMRELAAYIWVAFQAWIVMKLIIFYFGIEAADNPQESHWLLYA
                     AILFSFGSLVLFAWRKFKKEE"
     gene            1549..2418
                     /locus_tag="VIG33_17570"
     CDS             1549..2418
                     /locus_tag="VIG33_17570"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY8272708.1"
                     /translation="MFLFIYILIGIFLGPSFVNIPASQLGTSSLDLLFSLCSFGFLFI
                     AGLELKVEALRKDFLSSAKIAAGAFALPFAVGLIFFQGSSVVALALAISALPVVVQIL
                     KDLKLYETRQGHLIVASATLCDIFAWLLFTVAIPNDARGSWILSHLPVFFFFLGLGMS
                     RGARRSETLLKIVLGSSRFFFGPVFFIGVGMKIHWQESFDLNQCLTILGVASISKMVG
                     VYLTSRAIKFDHRDATLMAIVLNARGAMEILFCSIALKLGLIDSTLFTSLTLMAVLSS
                     VIASPLVKLFIAL"
     gene            2515..3468
                     /locus_tag="VIG33_17575"
     CDS             2515..3468
                     /locus_tag="VIG33_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272709.1"
                     /translation="MSRNTLLLILTILFSMAVFWYFKSSQLESSEITQKAMPVRFSEV
                     VESGASRIPQVAPLERTPPLSDSKENQRVEREREIAHLQSQLAEESQKLEEQSKMLEQ
                     LRQSRQNAPVAASYRSSISSRDLEIQYLTETLGTYRQAEQDMNRSAALALRNQDHGAQ
                     VARDQLERDIQNLELGILNTQNELNYWLNYSSGVDFIRQRSEIERLQNQLAQQQNELT
                     PLRAQRVDISAQVLNNSQSIEALTERAKEEIRNSAAETQGQILSLRSEINRLQEMQSQ
                     NQSQLSALDSQLRKTEKDYEERSNKVRSLEETLRAKQAELH"
     gene            complement(3680..3934)
                     /locus_tag="VIG33_17580"
     CDS             complement(3680..3934)
                     /locus_tag="VIG33_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272710.1"
                     /translation="MDFTKAMGIFLFLLKVLKRHGLNRCLQFQSKRMHIDGLQEIQAV
                     DGFKVFLEHDASLNAQTHIINFKHRDLAPKPAVKSEEKKG"
     gene            complement(4148..4660)
                     /locus_tag="VIG33_17585"
     CDS             complement(4148..4660)
                     /locus_tag="VIG33_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272711.1"
                     /translation="MRQIDFGFLQKQSLSFGGSSLLGKRKTARPLSTKKPLHLVLKSE
                     SSSLFNPGNRALEKLIRDHAKKYSIAVYAFSFNWSHLHLVLKIPTRGSYMAFVRTVTA
                     AIISFLSKQKGRTLKGTFTLRPFTRILNWGRDLRNVINYNELNDLEGWGLLSRNKKTT
                     PTPQSPQGTG"
     gene            5349..6152
                     /locus_tag="VIG33_17590"
     CDS             5349..6152
                     /locus_tag="VIG33_17590"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEY8272712.1"
                     /translation="MNFNFTKSFCGFSFVLFILTFQNCSKARFVADVNAEALSSLTTN
                     GNGDDGNCRPNAMTTNKIIKVLFVVDTSGSNGGATATDLNKKWRSATITNFMQQYSGK
                     SNFYYGLITFQGNSAQGQIMVNGTPGFSNDMNVVDSGYANFLATPDSGSTPYKAALSM
                     AKNIISADFMENASQNASYVMVMVSDGQAKDYKSPDEVIPDAKALTAVAPGQVSLNSV
                     YYYSTILDDSQTLYLRNISSVGGGSFIIANSNEVLSINDTIQLPPSSCH"
     gene            6232..6414
                     /locus_tag="VIG33_17595"
     CDS             6232..6414
                     /locus_tag="VIG33_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272713.1"
                     /translation="MKLTEELGEEGRKSLFEDIIMNKEVSYLKTSYCSAERKATTDLV
                     AEYERIISQSTKLLLN"
     gene            6563..7126
                     /locus_tag="VIG33_17600"
     CDS             6563..7126
                     /locus_tag="VIG33_17600"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEY8272714.1"
                     /translation="MARILIVYESKYGQTEKIAKFIVNRMLSQGHSVNLMNLRTKDLQ
                     SPANYEGIIVGGGVYMRSYPKSLVKWVQENSRNLSQKPTAFFSVCLAVMQNDLRTQRD
                     LHLIENNFFKKTKWFPKRREAFAGALSYSKYGWLTKRVMKLMAQSRGGDSDTSRDYEY
                     TSWKDVSRFSDNFVNSLRQMSNEMHAP"
BASE COUNT         1977 a         1374 c         1676 g         2105 t
ORIGIN      
        1 tagaagaatt tgcgcgtccg cctgttgagt ttttttcaac tgcaaacgct tgctcgccaa
       61 ttcagcctta atcttggacc gatcagcttc ttgatttaaa gagcgttgtt gtcgttccag
      121 atccttaatg tcgctgctaa gccgattaat ttccatgaca gtttgtttct cggcggcttg
      181 atactcttcg ttatcaagag tgctgacttc agattctaaa gctaaagttg ctttttgctg
      241 attcatagta ttcatcgtac tgttaagttc agcagagagt gccgggttgc tctctgtgac
      301 ttcacggctc catgcattta aactccctag tactaatacc atagcaaggg tgcgtttaag
      361 tagtgtattg cccattcatc ctccaagttc tatacaatgc tttaaggttt ctagcaatct
      421 ccgtacctgt ccaataacct tcaggatgga ccagcgcaat ttgagccgtg aagtttattc
      481 cagaaactga ctagaactgt gacagactga gtttgcctct taaggtgagg caggctatgc
      541 tatcaacatg actgaaaccc ttatttatgg ctttttattc gtacttggat tttgccttat
      601 tattttccga ccgattgaga gatcgatcgg ggtctctcct tattgggaga ttggctttgg
      661 atccctttta gtcatatttt ttgccatttt tataacctca agctttattt tggaacccat
      721 gtttgaagaa gatcttgcaa agaaaccttg tggcggaagc agtcctcagg gaaattgtta
      781 cagtctggaa cattctctgt gcgagacggc ctggcaaggg gctgaaagcc agtgtaaggc
      841 tgaaatggac cttattttga aggctcgtcc ttcggcgctt attgggccaa ccatgaatcg
      901 ctgccgcgca aagcgcatgg atcaagcgct cagatttaat cgtataaaca cacatacgag
      961 ctattgcaaa gcctactttg aatatattga taggaagtaa ggtatggcgg aacttattga
     1021 aaatattttt atggactctt ttaagcggga gtttgatcta gggtcaatga cggccgaggc
     1081 ccaggatttg gtgaaaacca tgagcaaaca acaaaatgct gattttgcga gtagcagtgg
     1141 aggacatttt agcgctgaaa gtcgggaatt tattgcgaaa gaatccgcct ttcgcttggt
     1201 aaaggccttg agttcaaaaa atgcaactga agtggatcgg gatttacttc aacaagaatg
     1261 gaacaagatt gttagagatt tttatcagct gaacttctgg gggcaggtgc cacagaaaag
     1321 taaacccgcg aaggttaaaa cagaagaaca gaaacgaatg cgggagttgg ctgcctatat
     1381 ttgggtcgca tttcaagcct ggatcgtaat gaagcttatc attttttatt ttggtattga
     1441 ggccgcggat aatccacagg aaagtcattg gttattgtat gcagccattc tattttcctt
     1501 cggatccttg gtgcttttcg cctggagaaa gttcaagaaa gaagaataat gtttcttttt
     1561 atttatatcc ttatcggaat ttttctaggc cctagcttcg taaatatacc cgcatcccag
     1621 ttgggaacgt cttcgctgga tttgctattt tccctgtgtt ctttcggttt tctttttatt
     1681 gcgggtttag agctgaaggt cgaggctctg cgtaaagact ttttatcttc ggctaaaatt
     1741 gccgccgggg cttttgcgct gcctttcgcg gtggggctga tcttttttca gggcagcagt
     1801 gtggttgcct tggctttggc tatttcagct ttgccagttg tggtgcagat tcttaaagac
     1861 ctgaagctct atgagactag gcagggccat ctgattgtcg cctccgctac tttatgtgat
     1921 attttcgctt ggttgctttt cactgtggct attcccaacg atgctcgcgg gagttggata
     1981 ctcagccatc ttcccgtctt tttctttttc ttaggccttg gcatgtcaag gggagccagg
     2041 cgttcagaga ccttactgaa gatcgtactt gggagtagtc gattcttttt tggaccggta
     2101 ttctttattg gggttggaat gaagattcac tggcaagaga gttttgactt aaatcagtgt
     2161 ttaactattt taggggtggc aagtataagt aagatggtcg gagtttattt gacctctaga
     2221 gctattaagt ttgatcatcg ggacgccact ctcatggcca ttgttctcaa tgcacggggc
     2281 gctatggaga tacttttttg ttccattgct ttaaaacttg gattgataga ctccactctt
     2341 tttaccagcc tgactttgat ggctgttcta agttccgtga tcgcctcacc cctcgtgaag
     2401 ttgtttattg ctctctaata ctgtctgaaa gcaagtgctt tgcttgtttt attaactgga
     2461 aagtgtgtgg gtttagaaat ataatggaaa gcagaggtaa taagaggcgg ctctatgagc
     2521 agaaataccc tcctgttgat tttgacgatc ttgttttcca tggcggtgtt ttggtatttc
     2581 aaatcttctc aattggaatc ttctgaaatt actcaaaagg ctatgccagt gcgtttttct
     2641 gaggtcgtag agagtggtgc ttctcgcatc ccgcaagtgg ctcctctgga gcggactcct
     2701 cccttgagcg attcaaaaga aaaccaaaga gtagaaaggg aaagagagat tgctcatttg
     2761 cagtctcagc tggcggaaga atcacagaag ttggaagagc aaagcaaaat gttggaacaa
     2821 ctgagacagt ctcgtcaaaa cgctccagtg gcagcttctt atcgttcctc catttccagt
     2881 cgagatttgg aaattcaata cctcaccgaa accctcggaa cctatcgcca ggcggaacag
     2941 gacatgaatc gatccgccgc tctagctctc agaaatcaag atcatggagc gcaagtcgct
     3001 cgagatcagt tagaaaggga tattcagaat ctcgagctgg gaattctcaa tactcaaaat
     3061 gagctgaatt attggctcaa ctactcctca ggcgtggatt ttatccgtca aagatcggaa
     3121 atagaaaggc tgcaaaatca gttggctcag caacaaaatg aattaactcc gttaagggct
     3181 caaagagtgg atatttccgc gcaggttttg aacaattctc aatcaattga ggctttgacg
     3241 gaaagggcga aggaagaaat cagaaattcg gccgcagaga ctcaggggca aattttatcc
     3301 cttcgcagtg aaatcaatcg tttgcaagaa atgcagtctc aaaatcaaag ccagctatcg
     3361 gcactcgatt ctcaattgag gaagactgaa aaagattacg aggaacgctc caataaggtg
     3421 cgttctcttg aagagaccct cagggcgaaa caagctgagt tgcattagga gggggaagag
     3481 ggccgatgga gatctgttag agatcgactt ctcgaaaata atgatttttt agttccagtc
     3541 gcaaatccga aagttgaaat tttagagtgt taccaaatgg tactaacccg ggagaaattt
     3601 ctttttctac ggagaaataa atattaaggc cagcttgcgt tccaaatcgt ttttattttt
     3661 ggctttagta agttgataat tatccctttt tctcttcgct tttgacggct ggcttaggag
     3721 ctaaatcacg atgcttgaag ttgatgatat gagtttgagc atttaggctc gcatcgtgtt
     3781 ctaaaaaaac tttaaatcca tcgacggctt ggatttcttg aagaccatct atatgcattc
     3841 gtttgctttg gaactgcagg caacggttta gaccgtgcct cttcaaaact ttcagcagaa
     3901 ataaaaaaat tcccatggct ttcgtaaagt ccatcgcaaa tctcggtgtc gtaaaatccg
     3961 caatgaacga gatataactt catagagtct ccttgccact ccaagagcag gagtggagga
     4021 gaattaggat tgctcttgtg gaaatggtgt gcacggaaat ttcgcacatt gaatcttatt
     4081 tattttcacc ttcagttgag attgagacct tcgctggagc gagcatcagt tccctctttt
     4141 tttctttcta gccagttccc tggggagact ggggtgttgg agttgttttc ttgttccgtg
     4201 aaaggagtcc ccagccctca agatcattca attcgttgta gttgataaca tttcgtaagt
     4261 cccttcccca attcaagatc cgcgtaaaag gacgaagggt gaaagttccc ttaagagttc
     4321 ttcccttttg ttttgagaga aaagaaataa tggctgcagt gacggttctg acaaaagcca
     4381 tataacttcc tcgggtggga atttttaaaa ctaaatgaag atgactccag ttaaaggaaa
     4441 aagcgtaaac agcaatggaa tactttttag catgatctct gattaacttt tccagggctc
     4501 ggttgccagg gttaaataag gaagagcttt cgcttttcag caccagatgc agaggttttt
     4561 tcgtagaaag gggccgggct gtttttcttt ttcccaaaag ggaacttccg ccaaagctta
     4621 agctttgttt ttgcaaaaaa ccaaaatcaa tttgtcgcaa aggacacctc cgaaaaactt
     4681 tatatcacaa cttctgagaa gcggttttag gagaggggtg aggtgctata tggcttcctg
     4741 tttatgcaac ggaggacctt cgccctgaag agatcttggg ctgagttaaa gcttttgggg
     4801 tgagagggaa aaaagttatt tgtattgaat tcttgagagg ggaaagtgcc gtcaacttgt
     4861 tttttcttgg agaagaattt ttttgagggg gataaagctt cagaggctct ttcgatcaag
     4921 aggatcaagg aatggattaa gggaaagagg aaaagaaatg tctggtttaa ataagtcgcg
     4981 aaggattttg gaatatgaag agaaaatgtg ggcgcgactt tacttctgat tgcgtacaat
     5041 cagaagtccg gtaaaagtat gggagaaagt atgggagtat cgggtaaaaa aggagttata
     5101 gtgattatgg aagcaattta agcagctttt gcctgcaatg gatgtctggc ctcaagtgtg
     5161 cttaacccct atccccacaa atctcagaaa gaggcatgaa aagaggtaaa tgaaagaggt
     5221 aaatagatgt ctaagagtta ctcaaaaacc gcaacttcga cactgctgag tggaccgttt
     5281 caaaatagac ccctccagag cttacacgaa aattgcatgg taaaggatta gtaacaggcg
     5341 ggggccttat gaatttcaac tttacaaaat cattttgcgg attctctttt gttttattta
     5401 ttctgacttt tcagaattgt tccaaagcgc gcttcgtggc tgatgtgaac gctgaagcct
     5461 taagtagttt aactaccaac ggtaacggag atgacggtaa ctgtcggccc aatgcaatga
     5521 cgacaaataa aattataaaa gttctctttg tagtggacac gtcgggttcc aatgggggtg
     5581 cgacggcaac tgaccttaat aaaaagtgga gatccgcaac gattacgaat tttatgcagc
     5641 agtacagtgg aaaatcaaat ttttattatg gcttgattac tttccaaggc aattcagctc
     5701 aaggacaaat tatggtgaac ggaactcctg gcttttcaaa cgatatgaac gtagtggaca
     5761 gcggctatgc aaattttctc gcgactcccg attctggctc cactccctac aaggccgctt
     5821 tgtcgatggc taaaaatata atttccgctg acttcatgga aaatgcgtct caaaatgcct
     5881 cttacgtaat ggttatggtg tctgacggtc aagccaaaga ctataagagc ccagatgaag
     5941 tgattcctga tgcgaaagct cttactgctg tggcaccagg acaagtcagt cttaacagtg
     6001 tctattacta ctccaccatt cttgatgatt cccaaacatt gtatctcaga aatattagca
     6061 gcgtgggggg aggaagcttc attattgcca attccaacga agtcttgagt atcaatgata
     6121 ctattcaatt gccaccttcc agctgccatt aaagtatttt tcttggccac ttacgcttaa
     6181 attaaaccct ctgggggccg tcgccgaagt ccagttgacg gcgatgtgca aatgaaatta
     6241 acggaagaat taggagaaga ggggagaaag agcctctttg aagatataat aatgaataaa
     6301 gaagtctcct acttaaaaac ctcctactgt tccgctgaaa gaaaggcaac aactgatcta
     6361 gtggctgaat atgagcgtat catttcacaa tcaacgaagt tattgttgaa ttaatctggt
     6421 taaaaagatc gttaaagcag aacattgatt tgtcatttct ggccaagacg caaatcttcg
     6481 atggtatagt ttgttaaaac acctggttca ttttctgcca aaaaaaccta caatagaagt
     6541 cggttatatc tcggaggttt ttatggcacg gatcctcatt gtttatgaat caaaatatgg
     6601 acagactgaa aagattgcga agtttattgt gaacagaatg cttagccaag ggcattccgt
     6661 gaatttgatg aatttgcgaa ccaaggatct tcagtctcca gcgaattatg agggaattat
     6721 tgtagggggt ggagtctata tgcggagcta tccgaaatcc ttggtgaagt gggtgcagga
     6781 aaattccaga aacctgagcc aaaaacccac ggcatttttt tcggtgtgtt tggcggtgat
     6841 gcaaaatgat ttgaggactc aaagggatct ccacctgata gagaataatt tttttaaaaa
     6901 aactaaatgg tttcctaaaa gacgcgaggc cttcgccggg gctttgtctt attctaagta
     6961 tggttggctt acaaagcggg ttatgaagct catggctcaa tcgagagggg gagactccga
     7021 cacttccagg gattacgagt atacaagctg gaaagatgtt tctagattca gcgacaattt
     7081 tgtaaattct ctgcgacaga tgagcaatga aatgcatgca ccgtgactga gg
//
LOCUS       DATBTE010000461         2181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_610881, whole genome shotgun sequence.
ACCESSION   DATBTE010000461 DATBTE010000000
VERSION     DATBTE010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610881"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="VIG33_17605"
     CDS             complement(<1..1378)
                     /locus_tag="VIG33_17605"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEY8272715.1"
                     /translation="MKQLMFILIAIFFLPSLNLASPIDLKKIVLTEEIAKDAYLWGYP
                     IVRFERTKRLFTTTPGFGHAPLNSFYHATHLPNPEDKGMSNPLPDTLYSSAFLDLRNQ
                     PMILETPKIKDRFYSLQVMDAFTNNISFISSRTHGEAAGKFFITGPHFIGTTPPGFER
                     IHSSTNFVWIIGHIAVESPAQVKTAYSYLRKYDLRPYSVYLGKEKMQKNRLLTAKANL
                     TFDPRKLADAGVRYYDELGMALLENDPAGLDGPLLGRFRTINIGPGLMTSQYISVREI
                     REAYERAIASGEIEVDRSIKKDLIQNRNGWNFVSTRGEFNKYNTVRTALSKVYFGESS
                     AIESIHPVIYVDNNNVRLNGNFTYLLHFTKDKMPPAKAFWSIAAYDAHERNLVRNNLN
                     RYTLGSYSKNMVLNSDGSLDIYISANEPNGHIDNWLPVPRGNFYVIMNMYNPSDDVIS
                     GKYTLPSLQ"
     gene            complement(1467..>2181)
                     /locus_tag="VIG33_17610"
     CDS             complement(1467..>2181)
                     /locus_tag="VIG33_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272716.1"
                     /translation="QPLCSLQFLCEQLKLEKTLLSTAFYEENKNRTIDHLQEIFTDNQ
                     VSILRLLGHKKKALPPAQFSKLAEAILTVSLGSFEEPGIKNVFPGACNRPNAIYISGM
                     HRIFVCPSLLSYPDLTLQQILAHELGHVIQRMQDYVPCFYGYPKSQRDEVFADWVASK
                     VLAEKISSEKNKKIAGKQAFESQLLFLNLACGNVQKIKPKWNNTHPNLQNRVEHIFLS
                     QPAFQEALRCHSKYLRTCG"
BASE COUNT          650 a          406 c          488 g          637 t
ORIGIN      
        1 tttgtaaact aggtaaagta tatttaccac taatcacgtc gtcacttggg ttgtacatat
       61 tcattatgac ataaaaattt cctcgcggaa ccggaagcca attgtcgata tgaccgttcg
      121 gttcatttgc agaaatataa atatccaatg atccatcgga gttcagaacc atattttttg
      181 aataactgcc taaggtgtag cgattcaaat tatttctcac gagatttcgt tcatgggcat
      241 cgtaggcagc gattgaccag aaggctttgg ccggaggcat tttgtctttg gtaaaatgaa
      301 gaagataagt gaaattgcca ttgaggcgga cattattatt gtctacataa ataacgggat
      361 gaatggattc aatggcactg ctttcaccga agtaaacttt gcttaaggcg gttcgaacgg
      421 tattgtattt attaaattcc cctctagtac taacgaagtt ccatccgtta cgattctgga
      481 tgagatcctt tttaatagag cggtccactt cgatttcacc cgaagcaatg gccctttcat
      541 aggcctcacg gatttcacgg acgctgatgt actgagaagt cataagtcct ggccctatat
      601 taatagtgcg aaatcgtccc aacagagggc catctaaacc agccggatca ttttccagga
      661 gcgccatgcc cagctcatca taataacgaa ctcccgcatc tgcgagcttt cgcgggtcaa
      721 aagttaagtt ggcctttgct gttaaaaggc ggtttttttg cattttttct tttcccaagt
      781 acacgctgta gggacgtaag tcgtattttc gaaggtagct ataagcggtt tttacttggg
      841 cgggagattc tacggcaatg tgtccaatga tccaaacgaa attcgtcgaa gagtgaatac
      901 gttcaaatcc tgggggtgta gtcccaataa agtgcgggcc cgtaataaaa aacttgcctg
      961 cagcttcacc gtgagtacga gaggaaataa aagaaatatt attggtaaag gcgtccataa
     1021 cctggaggct ataaaaacga tccttaattt ttggtgtctc taagatcatc ggttgattgc
     1081 ggagatctaa aaaagcagaa gaataaagag tgtcgggtaa ggggttggac attcctttat
     1141 cttcaggatt gggaagatga gtggcgtgat aaaaactatt taaaggagca tggccgaaac
     1201 caggcgtcgt agtgaacaat ctttttgttc gttcaaagcg tacaatggga taaccccaaa
     1261 gataagcgtc tttggcaatc tcttcagtca gtacgatctt tttaagatct ataggtgatg
     1321 ctaaatttag gctcggaaga aaaaaaatag ctatcaaaat gaacatgagt tgtttcacag
     1381 ggcctccaga agaacatgcg ttgcatacta gcacggaggg gaatcagatt ttggttgaga
     1441 tctagaagaa cttaagaaaa ataatactat ccacaagttc ttaaatattt ggaatggcag
     1501 cgcaaagcct cttgaaatgc aggttgagaa aggaaaatat gttctaccct attttgcaag
     1561 ttggggtgag tattgttcca cttaggtttt attttttgaa cattaccaca ggcaaggttt
     1621 aagaaaagca gttgagattc aaaagcttgt tttccagcaa tttttttatt tttctcagat
     1681 gaaatttttt ctgccagaac ttttgaagcc acccaatcgg caaagacttc atcgcgttgg
     1741 gattttggat aaccgtaaaa gcacggcaca taatcttgca tcctttgaat tacatgaccg
     1801 agctcatgtg ctaggatctg ttgcagcgtc agatctggat aactcaacaa agatggacat
     1861 acaaatatgc gatgcatgcc agaaatataa attgcattgg gtcgattgca agcgccaggg
     1921 aatacatttt taattccggg ctcctcaaag cttcccagac ttacagtaag gatcgcttcc
     1981 gccaactttg aaaactgggc tggaggtaag gccttttttt tgtgaccaag aagtctaaga
     2041 attgaaacct ggttatcggt gaatatttct tgaaggtgat cgatagttct atttttattt
     2101 tcctcataaa acgcagtgga aagaagagtt ttctctaact tcagttgttc acaaagaaat
     2161 tgcagggaac agaggggttg t
//
LOCUS       DATBTE010000462         2960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1089552, whole genome shotgun sequence.
ACCESSION   DATBTE010000462 DATBTE010000000
VERSION     DATBTE010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089552"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..264
                     /locus_tag="VIG33_17615"
     tRNA            181..264
                     /locus_tag="VIG33_17615"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:214..216,aa:Leu,seq:cag)
     gene            complement(320..1699)
                     /locus_tag="VIG33_17620"
     CDS             complement(320..1699)
                     /locus_tag="VIG33_17620"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEY8272717.1"
                     /translation="MIKCLTSLIIISAWMTCAEAARPSFNSKPWINDYSELRQHLEKS
                     YANLTWFASPQGELDLPKLHRKTLSALNNSKSASEAKAVIESFVEAFYDGHLYILPKA
                     STLPDTNKTLVSPLSRSSSSTLACAQLDFVHKKGTAFSLPFEVSPGFELLNSGESDSF
                     RTGIANLPNGKKIGILRVSVMRQNEFPNECERAWNKVTRNQKSGVCDSTCKEEVNDRA
                     MEFLLEDLSQRLHAFSKLGVSAVLLDIGGNSGGNDLGDWIPRLFTSREIHSPRLGIVT
                     DPKSVVYYDEQLAGISSALKGKNSSTALSALNNAADEFKRLKELALNIKKCDMSWVWR
                     ERRAWFPLDSKCTNLSFGQSYFSGALDFLKSGTLGSPEVEKSLYWPTIVSPYIGSWVG
                     PVYVVVDKKTGSAAEATAAIFQNNKAAKIIGSKTAGYGCGFMYKDSEFTLSNSRLRVS
                     IPNCVRLIS"
     gene            1823..2017
                     /locus_tag="VIG33_17625"
     CDS             1823..2017
                     /locus_tag="VIG33_17625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272718.1"
                     /translation="MLEEITAYFAIDRKKCFEVMEFLTLVGLCDLKDHHYHMGTQHTH
                     VAANSPHAIKHHLNWRIKSL"
     gene            2684..>2960
                     /locus_tag="VIG33_17630"
     CDS             2684..>2960
                     /locus_tag="VIG33_17630"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY8272719.1"
                     /translation="MKLFLSLALVLNVSTVLAGTQKLEDIEKTKKIHKITGALTTNEY
                     QLANGLQVFLTENPKAPEVVISHWVKAGSLNESPGITGIAHLFEHMMF"
BASE COUNT          872 a          674 c          618 g          796 t
ORIGIN      
        1 attgggacac cctggaaaag ttcttaaaaa taccccattc ccgtattgtg catctgtatc
       61 acaacaagca cgaagtcact tggatttgca cccaaaccgg aaatgagctt tgacttcagc
      121 aatcagcaag ttgagcttga attttctcaa aagtaccctt agtctacttc ataccttcaa
      181 gcgcgggtgg tggaatggta gacacgcagg tctcagaagc ctgtccgtaa cagggtgagc
      241 gttcaagtcg cttcccgcgc accattattg aagtatttga cggtcattga tcgattttag
      301 aattcatatg cggagtgtcc taagaaatta gacgaacaca atttggaatg ctaacacgta
      361 gtcgcgaatt agaaagcgta aattcggaat ctttatacat gaagccgcac ccataaccag
      421 cggtttttga tccaattatt ttagccgctt tattattttg aaaaattgct gccgtcgcct
      481 ctgccgccga acctgttttt ttatccacaa ccacatagac ggggccaacc cagcttccaa
      541 tatatggaga cactatcgtt ggccaataca gacttttttc cacttcaggc gaacctagag
      601 ttccactttt tagaaaatca agggctccgc taaaataact ctgtccgaaa ctcaaatttg
      661 tgcattttga atctaaagga aaccacgccc tacgttcgcg ccaaacccaa gacatgtcac
      721 acttctttat attaagagct aattctttca atcgtttaaa ctcatctgca gcattattga
      781 gtgcagataa agccgtcgat gaattcttgc ctttcagagc tgaactaatt ccagcaagtt
      841 gttcgtcata atacacaaca gacttgggat cggtcactat cccaagtctt ggcgagtgta
      901 tctcgcgaga agtaaatagg cgtggaatcc aatctcccaa atcgttaccc ccgctatttc
      961 cgccaatatc gagcagaaca gcggacacgc ctagttttga aaaggcgtgc aagcgctgag
     1021 atagatcttc cagtagaaat tccattgcgc gatcattcac ttcttcctta cacgtagaat
     1081 cgcatacccc agacttttga tttcgagtaa ctttattcca ggcacgttca cactcgtttg
     1141 gaaattcatt ttgccgcatg accgaaactc gcaatattcc aatctttttt ccatttggca
     1201 agttggctat tccagtgcga aacgaatccg attcaccaga attgagcagc tcaaatccag
     1261 gactgacctc aaatggcaaa gaaaatgcgg tacctttctt gtgaacaaag tccaattggg
     1321 cgcaagcgag agtggaacta gaagatctac tcagaggtga aactagggtt ttgtttgtgt
     1381 caggtaaagt agaagctttc ggaaggatat acaagtggcc atcataaaat gcttcaacaa
     1441 aagattcgat cactgccttg gcctctgagg cgctcttgga attatttaat gccgacaacg
     1501 tttttctatg aagttttgga agatctaatt caccttgtgg cgatgcaaac caagttaaat
     1561 tcgcataaga tttttctaaa tgttggcgaa gctcactata atcattgatc catggtttgg
     1621 aattgaaaga aggccgggct gcttctgcac aagtcatcca agcagaaatg ataattaacg
     1681 atgttagaca ttttatcata aagccaatat acagcgacga gataacgtgt caattaggcg
     1741 attcaagggg caatcatgca acccttcgat tggagccgag gccatgcttc ggccttggtt
     1801 tgaaacgcgc tgacaattgt tcatgcttga agagatcacg gcctacttcg ctatagaccg
     1861 aaaaaaatgt ttcgaagtga tggagttctt aaccttagta ggtctttgtg accttaaaga
     1921 ccatcactat catatgggca cacaacacac gcatgttgcc gcaaactctc cccacgcgat
     1981 taagcatcac ttaaattggc gcatcaagtc gttatagcgc caagaaaatg ttcaggagca
     2041 agagctagtt ttcacagccc ctatgtcttt atctgaaaaa gactttgtga ttattcgaga
     2101 aaagcttaca gccacactta aagattgcat tgaagttgcc aaggagtctg aatccgaaca
     2161 actttctttt ttaacaatag attggcttaa aatttaatct ctgaaagtcg ctatcaggtc
     2221 gattttacgc agaccatata tagatgctga ggctgacggt caccaaagcc ccttatatag
     2281 attctggcgc cgccgaggtg gcgcacgggc cgaaaccaat tcctacctta aaaaaaccgg
     2341 cggcggcaaa aaaccacgct gacttaaagg ggcgcatttc cgagcagagg gggcaaaggg
     2401 ttcgatcagg ttgtcagcgc cgccgcgaac gccgagttgc ttaaaaggat tcctttgatg
     2461 agaaatttgt atccggagtt aaatatactt aaatttcact cactgaagac cattcacaaa
     2521 tgaatggcga atgcatcaag tttccggctt cgacaatgaa atcacctgga gcaataagca
     2581 tgaattttct taccctgaaa gcacggtgtc aaaccgtgcg cctcccaaaa ctccatgctt
     2641 aacttcgtcg taccattaat tggtgtttag cgaaggagat cgtgtgaagt tgtttttgtc
     2701 attggccttg gtgcttaacg tctctaccgt gttagctggg actcaaaagt tagaagatat
     2761 cgagaagacc aaaaagattc ataaaattac aggtgccctc acgaccaatg aatatcaatt
     2821 ggccaatggc ttacaagtat tcctgacaga aaatccaaaa gcacccgaag tcgtcatctc
     2881 tcactgggta aaagctggaa gcttaaatga atccccgggg atcacgggta tcgctcacct
     2941 gtttgagcac atgatgtttc
//
LOCUS       DATBTE010000463         4983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1089615, whole genome shotgun sequence.
ACCESSION   DATBTE010000463 DATBTE010000000
VERSION     DATBTE010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089615"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VIG33_17635"
     CDS             <1..615
                     /locus_tag="VIG33_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272720.1"
                     /translation="AELCAQCLEEEDANKADTSSEGLIKSRIDRLIMSLEKDTSPASS
                     LREPLTRVQQCEPFLLCLDDCFSYMLTSPGVTLALVSSELSEYIAVMRARAREFLKLK
                     EAVDSGRTLQILYLANLVAESNDGDLVEFLRELLRYHKQCMTERGRDPWIFLEADKLI
                     VVVSADMDKQRARKRLKSGTPWENEYYLWTVDTIYKQLFGGSNV"
     gene            608..2626
                     /locus_tag="VIG33_17640"
     CDS             608..2626
                     /locus_tag="VIG33_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272721.1"
                     /translation="MSDAIGAIGESPMREAFELMLKQFKESQPDYVLFTTFNFSSSFF
                     ESNVLPLLAGNSADELRGGGEVRNQINTFLEQTKTLVVCDRSTSPEPKGDFRYGLLPV
                     GLENGRFHPKIVLMAGKLVSGMDGLWLSVASGNLSLSGWAINREVIGITPIAEKHRDE
                     LQILLDWLLKEANRRIDWVSDVGQDLTEEGDSRQILQSLINAINALDRDVSLDELASE
                     WPDLHLALPPFVRTMDKTPAKPLLAALGGDEEWTSATVISPYWAALSQLIKTVKVKRW
                     RFIPSLGQDGTYGFPLNDLPTESDKNTYSFQKFLDGERFTHAKAILFEREGANAKRVL
                     CIGSANFTAAALFHGEGRLSNIEAMLCYQGLNSDPWVGLFETLDKKKVGVMDDTDVDK
                     GAPPLPPFDAEVVYDWKKGQFSCRLMIHEGVNLQNVRFEVAKESLPMKASTKVEQVKK
                     ILFRGRTVVRSFQVIYSEIAGTELHYQGLVTQLNAHDDQLGYRSPPRLTMVLDLLRSL
                     DPEASEREIRRRAERGGDLDSDRDEQEESSFDFFTFFQATYKMRKFYAKHSDLDPFLA
                     SAPQGIPNLFRAIVLQPVANEEAEVGRYVQLAELLETAESFAKQANFQCQNVNDCLNS
                     IREELEEIEEKMRALIESSNALRVMFGRNAGETKGHRFLRWFREELKE"
     gene            2632..>4983
                     /locus_tag="VIG33_17645"
     CDS             2632..>4983
                     /locus_tag="VIG33_17645"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEY8272722.1"
                     /translation="MSMRNNLVPCPLSPRQVRSYINFDGGTEVDLEGTSQSRLQSQGV
                     AALWELLESKNFAYLGDEVGMGKTRQAMGVIATQFLKKPESHVVIICPGETLQNQWLR
                     EWGEFMRTCYKGYDDRLKSAVDGAALHALHRHDHLHDFAKALLLNESHIHLLRYSSFS
                     RPIWFGSAETLGDVRKEFEQCLTEIGISQISEEERSIFNKYSTKNIENWRSMMTSDLN
                     ECYAKRVGELFRARDIDLVVFDEAQYLRNIGNRQNTNIRHIFRHYVHKWLFMSATPLH
                     SGYKDIQSLDYYLCDKIGNFAPGKNLCSGCQRPNKCTRATYRLESKSPEKIDVVDLLK
                     EFLVRRPRSYQDYNKDKYSKVQYRKYKRSKIAVAKDPFLALTMALVQKHLVKTLEGGN
                     NKFRQGECSSFESLSSSIKSKGKCLDKDDMSKEESELEKVGPSIDMPKQETTLDSSFI
                     DALNISFRAAMMSKATLESHHHDDVDNPKYDMPHAKLNEIASSLFQSHFRNSSNSKAL
                     VFVRRLDTVEELIDLLSSQFQDEVDRRLECWRSSLQRHGNMIDFRESLWREGEFWSKE
                     DDSADDAEQEDDLIYGSTNDGSPETERVVRLPYFDALRTYKKIDKEEQSNLPNGMLSS
                     FQSRLLRVKKISGNPFHGFLLTRPDFEGAAESDTNEIELAWKKADNFWETFVMHVVGQ
                     SSGSDLDEKYQWLVKPQDSRNDAFWKLAALKGCILHSMRHTDFLVDLYVLNRYVKKIN
                     DGRELTALPEKLLWLFKPNREPKLEADLEEYIGNWRIKFKLWFD"
BASE COUNT         1358 a         1022 c         1315 g         1288 t
ORIGIN      
        1 gcagagctct gcgctcagtg tcttgaagag gaggatgcaa acaaggctga tacttcctcc
       61 gagggtctga tcaaatcgcg tatcgataga ctgatcatgt cgctagagaa agatacttct
      121 cccgcatcca gcttgcgtga gccgctgact cgtgttcagc agtgtgaacc ttttcttctt
      181 tgtttggatg actgcttcag ctacatgcta acatctccgg gggttacgct tgctttggtt
      241 tcaagtgaat tgagtgaata cattgctgta atgcgcgctc gggcaaggga atttctgaaa
      301 ttgaaggagg ctgtggattc aggacggact ctacagattc tgtatttggc taacttagtc
      361 gctgaatcaa acgatggcga tttagttgaa ttcttgcggg aactactgcg ctaccacaag
      421 cagtgcatga ccgagcgcgg ccgcgatccc tggattttcc ttgaggcaga taagcttatc
      481 gtggtcgtct ctgctgacat ggacaaacag cgtgccagaa agcgactcaa gagtggtacg
      541 ccttgggaaa atgaatacta tctttggaca gtcgacacaa tctacaagca gttatttggg
      601 ggcagcaatg tctgatgcca ttggagcaat tggcgaatcg cctatgcggg aggcattcga
      661 actgatgctc aaacagttca aggagagcca gccagactat gtgctcttta ccaccttcaa
      721 tttttcgtcc tcattttttg agtcgaacgt attgccctta ttagcaggca attctgctga
      781 cgaattgcgc ggcggaggag aagtccgaaa ccaaattaat acgttcctgg aacagaccaa
      841 gactttggta gtttgtgacc gctctaccag tccagagccc aagggggact ttcgttatgg
      901 cctgttgcct gtcgggctgg agaatgggcg gtttcatcct aagattgtgc tgatggctgg
      961 aaagctggta tcgggaatgg atggtctttg gttatccgtt gctagtggca atttgtcgct
     1021 gtctggctgg gctataaatc gcgaggtgat aggcataacg cctattgcag aaaagcatcg
     1081 ggatgaattg caaattctgc tggattggct attaaaagaa gcaaacaggc gaattgattg
     1141 ggttagcgat gttgggcaag atttgacaga ggagggagac agtcgccaaa tactgcagtc
     1201 cttgataaat gcgataaatg cccttgatcg agatgttagt ttggacgaac tggctagtga
     1261 atggcctgac ctacatttgg cgttaccgcc ttttgttcgc acgatggata aaactcctgc
     1321 taagcctttg cttgccgcct tgggtggaga tgaagagtgg acatcagcca cagtaatctc
     1381 cccttattgg gcagcattat ctcagctaat aaaaacggtc aaggttaagc gctggcgctt
     1441 catcccttct ttgggacaag atggaaccta tgggtttccg ctcaatgatt tgcctacgga
     1501 atctgacaaa aatacctatt cttttcaaaa gttcctggac ggcgagcgtt ttacacacgc
     1561 gaaggcgatt ttgttcgagc gtgagggggc aaatgctaag cgagttttgt gcattggctc
     1621 cgcaaacttc acggcagccg ctctgtttca tggcgaaggt aggctctcaa atattgaagc
     1681 catgctttgc tatcaagggc ttaacagcga tccctgggta ggactgtttg agaccctcga
     1741 taagaaaaag gttggcgtaa tggacgatac cgatgtagac aaaggggcgc ctcctcttcc
     1801 accatttgat gcagaagtgg tgtacgactg gaaaaaaggg cagttttcct gtcgccttat
     1861 gattcatgaa ggtgtaaacc tccagaatgt aagattcgaa gtagctaaag agtcattgcc
     1921 aatgaaggcg tcgacgaaag ttgagcaagt gaagaaaatt ctctttcggg ggcgaactgt
     1981 tgtccggagt tttcaggtga tctattccga aatagcagga acagagttgc attaccaagg
     2041 actggtcacc caattgaatg cgcatgatga tcaactagga tatcgttcac caccacgctt
     2101 gacgatggtt cttgatttac ttcgtagttt agaccctgag gcaagtgagc gggaaatcag
     2161 gcgcagagca gagcgcggag gggacttaga cagtgatagg gatgaacagg aggagtcgtc
     2221 ctttgatttc tttacatttt tccaagcaac ttacaagatg cgcaagtttt acgctaaaca
     2281 ttctgatctt gatccctttc tagctagcgc accacagggc attccgaatc tgttccgtgc
     2341 tattgtcttg caaccagtgg ccaatgaaga ggccgaagtg ggacgctacg ttcagttggc
     2401 agaactattg gaaacagcgg aatcttttgc taaacaagct aatttccaat gccaaaatgt
     2461 gaatgattgc ctgaactcga ttcgcgagga gttagaggag atagaagaaa aaatgcgagc
     2521 actgatcgaa tcatccaatg ctttgcgcgt gatgtttggt cggaatgccg gggagacgaa
     2581 aggacacaga ttcttacgct ggttccggga agaattgaaa gagtaaatcg tatgtctatg
     2641 cgtaacaatc tggttccatg cccgcttagt ccgcgtcaag ttcggtcata catcaacttt
     2701 gacggtggaa cagaggtgga tcttgaagga acgtcgcagt ccagactcca gagtcagggc
     2761 gtagcggcac tatgggaact acttgagtca aagaatttcg cctatcttgg ggacgaagtt
     2821 gggatgggca agacccggca ggcaatgggt gtcatagcga ctcagtttct taagaaaccc
     2881 gaatctcatg tcgtgattat ttgcccagga gagaccttac agaatcaatg gctgcgggag
     2941 tggggagagt ttatgcgcac ttgctataag ggctacgatg atcgtttgaa aagcgcggta
     3001 gatggtgccg ctctgcacgc tctccatcgc catgaccact tgcatgactt tgcaaaggcg
     3061 ttgctcctta acgaaagtca tattcatctg ctacgttact catctttcag taggccgatc
     3121 tggttcggta gtgcggagac actgggggac gttagaaaag aatttgaaca atgcctgaca
     3181 gagatcggga tctcgcagat ttcggaagag gagcgttcca tattcaataa atattctact
     3241 aagaatattg aaaactggcg gtcgatgatg acttccgatc tgaacgaatg ttatgccaaa
     3301 cgagttggtg agctatttag agcaagggat atagaccttg tggtctttga tgaggcccag
     3361 tatctgagaa atataggtaa tcggcaaaat acaaatattc gccatatttt tcgtcactat
     3421 gtccataagt ggcttttcat gagtgcgact ccgcttcatt ctggatataa ggacattcaa
     3481 agtcttgact actatctatg tgacaaaatc ggtaacttcg caccaggaaa aaatttgtgc
     3541 agcggttgcc aaagaccgaa caagtgtaca cgagctacct ataggctaga aagtaaatcg
     3601 cccgagaaaa tagatgtggt tgatcttctc aaggagtttc tggtaagacg tccgcgctct
     3661 tatcaggact ataacaagga taagtattca aaagtccaat atcgcaagta taaacgatcg
     3721 aaaatcgctg ttgccaaaga cccgtttttg gcactgacta tggcacttgt ccaaaagcat
     3781 ctggttaaaa cgttagaagg tggaaacaac aaattccgcc agggagaatg ctcatcgttt
     3841 gagtcgttgt cgagctctat taagagtaaa gggaaatgct tggacaagga tgacatgtct
     3901 aaagaggagt ccgaactaga aaaagtgggg ccaagcattg atatgccaaa gcaggaaacg
     3961 acgttggata gttcattcat tgatgctctc aacataagtt ttcgggctgc aatgatgtct
     4021 aaggctactt tagagagtca tcatcacgat gatgtggaca atcctaagta tgatatgcct
     4081 cacgcaaagc tcaatgagat agcgtcgagt ctttttcagt cacactttcg taatagcagc
     4141 aacagtaaag cccttgtttt cgtgcgacgg ttggatacgg ttgaagagtt gattgacctg
     4201 ttatcgagcc agttccagga cgaggtagac cgaagactgg agtgttggcg ctcgtccctg
     4261 cagcgacacg gcaatatgat agatttccgc gaatcactat ggagagaagg ggagttctgg
     4321 agcaaggaag atgattctgc ggatgatgcc gagcaggaag atgacttgat ctatggaagc
     4381 acaaacgacg ggagtccaga aaccgagcgg gtagtcaggt tgccatattt tgatgcactg
     4441 cgtacttata agaagataga taaagaggag caatccaatc tgccgaacgg aatgctgagt
     4501 tcttttcaaa gccgcctgct gcgagtgaaa aaaatatccg gcaatccatt tcacggtttc
     4561 ttgctgactc gaccggattt tgaaggtgct gccgagtctg atactaacga aattgagctg
     4621 gcgtggaaaa aagcggacaa tttttgggaa acattcgtga tgcatgtcgt gggtcagagc
     4681 tcgggttccg atctggacga gaaatatcag tggttagtaa aacctcaaga ttcccgcaat
     4741 gacgcgttct ggaagttggc tgcgttgaaa ggatgcatac tccattcaat gcggcataca
     4801 gactttctgg tcgacttgta tgtcctcaat cgatatgtga aaaaaataaa cgatggtcgc
     4861 gaactaacag cccttccgga gaaattgttg tggctgttta aacccaaccg ggagccgaag
     4921 cttgaagctg atcttgagga gtatatcggg aactggcgga taaaatttaa actttggttt
     4981 gac
//
LOCUS       DATBTE010000464         6040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_541990, whole genome shotgun sequence.
ACCESSION   DATBTE010000464 DATBTE010000000
VERSION     DATBTE010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541990"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VIG33_17650"
     CDS             complement(<1..401)
                     /locus_tag="VIG33_17650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272723.1"
                     /translation="MKSITEFAIFTLNKGLAAKAALAAEGKSAEEIQTSLGETFKLEG
                     DKLKYFVNALDVAGQNQQNLKRVFVISLNEGENAPAKATQVEELHYVPEFLNTAKPAA
                     TVDAKGGRGGKGGRGGGGRGEKKGGSPWGMTP"
     gene            569..1411
                     /locus_tag="VIG33_17655"
     CDS             569..1411
                     /locus_tag="VIG33_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HEY8272724.1"
                     /translation="MIKIGQINKLRVHKHVDFGLYLDDGENGEILLPRKYIKDNCQPG
                     DSLEVFICYDSEDRLIATTETPKAQVEEFATLKVVSTNSVGAFLDWGLPKDLFLPFAE
                     QTRRIAVGDQLLVYIYLDNTDRIAASMRIERNLDKTPDEYAVDQSVELIICGETDLGF
                     KAIINGKHLGMLYHNEVFQPLKQGQRLTGFIRNVRQEDGKIDLGLQKTGHHGAEEIGP
                     KILELLQKSNGFLAINDKTSAEKIYELFGVSKKKYKIALGGLYKKRLITVNEDGIRLL
                     EKEV"
     gene            complement(1496..3133)
                     /locus_tag="VIG33_17660"
     CDS             complement(1496..3133)
                     /locus_tag="VIG33_17660"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: protein motif:HMM:NF010584.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEY8272725.1"
                     /translation="MVERKAYLVGSGIASLASAAYLLREGRFLGQDIIIFDEDMDVGG
                     SLDGRGSSEKGYVLRGGRMLSFSTQCTYDLLSFIPSIKNPKKSVLDEIREFNEKLKSN
                     FSVNSRGHSQCRLVKNGQKLNPNILGFSYKDRFDLLEIMMKSEDSLGSRRIEDCFEKS
                     FFSTNFWYMWATLFAFQSWHSAIEFKRYLHRFIHEFSRLNLLAGVDRLPYNQYDSLVL
                     PLVKWLKSKGVNFMMETEVTDFDFTQIGVQKSISKLHYRRGEDTNSIDIAPADFVFVT
                     NGSVTASSSLGSMKTAPLLKSKKESAAWTLWETLAVNHPEFGIPAVFDNRVDESKWLS
                     FTVTLKDPEFFKLMEQFTGESAGASGLITFADSNWLLTLMLAHQPYFINQPSGVNVFW
                     GYGLFVDRPGNFVKRKMSECTGEEIMIELCSHLHFTLELPKILKSSNCIPCMMPFVSS
                     PFLTREKKDRPQVVPPTYLNLAFVGQYCEIPDDVVFTVDYSVRAAQTAVYKFLALDKA
                     PPPVYVSHRDFKALFDSLKTMFRADEKKPPEEHLGSP"
     gene            3421..3903
                     /locus_tag="VIG33_17665"
     CDS             3421..3903
                     /locus_tag="VIG33_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272726.1"
                     /translation="MKIFFASLIILGIPTEQVATAAAVHPTQKFITSFIQSVNDRLET
                     VNMERAIAGNRLYCSQLSDQQVKDIENLFISNPSAKANQFTYRSLDKVTIGQFITAAA
                     DNLGCYPTFWPTKGRSTIGGVLFNSKAFVMDHLLLQEALAALAGHATEDNQTLNTVLR
                     "
     gene            3999..5150
                     /locus_tag="VIG33_17670"
     CDS             3999..5150
                     /locus_tag="VIG33_17670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272727.1"
                     /translation="MLFASVLTLVFLSSSAYATDTASLEGLSVTEIGAIPKNSKVVLE
                     PVDPSKTYFQSANSGANGSEGFQTISPAAFYSVKSFSNSAPSTPDNATLQSLASMKSL
                     LIQQADLTQNSRNCVPSIAGLTCTLQNEVPAYKSGFFDFVAYLANRMNPTSWLPPDRT
                     TEVDSKPASDGQVVQALEKAVTQVNSKVSATQKSLSLNCETPPLPPPCGVQQALQAYH
                     AHKNEITKDIIVFNDFSDGKIMGKMWLLNSDGTPAKILQQNPIPVSRGPSGFGSGDGS
                     LKTPNGALLTKSYRPPREGNVKDGIELVGLDPDNKDVHSRGILLHGWNPYTSTEGCLG
                     VAGTLETGVHGKRTEGLPPYYLDELKKTVFKEGGVLIYDFTPQRKNLCK"
     gene            complement(5157..5408)
                     /locus_tag="VIG33_17675"
     CDS             complement(5157..5408)
                     /locus_tag="VIG33_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_038448462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEY8272728.1"
                     /translation="MNRDIFEGKFKEISGELKKKWGQLTDDDIRETEGNAEALAGIVQ
                     QKVGLEKDEASRTVSEILRNMERKFSIPKVSGRVNKKNS"
     gene            5578..5850
                     /locus_tag="VIG33_17680"
     CDS             5578..5850
                     /locus_tag="VIG33_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272729.1"
                     /translation="METTPNDLKESATSAGNYATEKARQLANAVSEKIQQQELKAEEL
                     YRTARVSAEDALDTSADFVKKHPFSTVAGAAALGFIAGILLRRTKH"
BASE COUNT         1702 a         1496 c         1220 g         1622 t
ORIGIN      
        1 ggagtcatac cccagggaga tccgcctttt ttctcaccac gtccgccgcc gccacgaccg
       61 cctttaccgc cacggccccc tttagcatct acagttgctg ctggtttcgc cgtattcaaa
      121 aactcaggaa cgtaatgcaa ctcttcaact tgagttgctt tcgctggggc attctcgcct
      181 tcattgaggc tgataacgaa gacacgcttc aagttttgct gattttgtcc ggcaacatcc
      241 aatgcgttca cgaaatactt aagtttgtcc ccttccaatt tgaaagtttc gccaaggctt
      301 gtttggatct cttcagcaga tttgccttca gcagcaaggg ccgctttggc tgcaagacct
      361 ttgttcaaag taaaaatagc gaactctgta attgatttca tggggacatc ctctctaaga
      421 taatgagctg cacataatgc gtataaatct tcttcaggtc aacgatgcaa aaacaaaaga
      481 tttttatatg atttgaccct ccgcatccgg tttgttagaa ttcggcccat tcaatcgtcc
      541 gtcctacttc ccatctatgg tgaattctat gataaaaatc ggtcagatca ataaactgag
      601 ggtccataaa cacgtcgatt tcggtctcta tctggacgat ggcgaaaacg gagaaatcct
      661 tcttccccgc aaatatatca aagacaattg ccagcctggg gatagccttg aggtatttat
      721 ctgctatgat tctgaggacc gcttaatcgc aaccacagag actcccaagg cgcaggtcga
      781 agaattcgcc acacttaaag tagtttctac aaattccgtc ggagctttcc tggactgggg
      841 acttcccaaa gatctttttc tgccgttcgc ggaacaaacc cgtcgaatcg cggtcggtga
      901 tcagcttttg gtttatattt atctcgataa cacggacaga atcgccgctt cgatgcgcat
      961 tgaacgtaat cttgataaaa cccccgatga atatgccgtc gatcaaagtg tggagctcat
     1021 tatctgcggc gaaacggatt taggttttaa ggccattatt aatggcaaac atttaggaat
     1081 gctctaccat aatgaggtct ttcagcctct aaaacaaggc caacgcctca ccggatttat
     1141 ccgcaacgta cgccaggaag atggcaaaat tgaccttggt ttacaaaaga ccggtcacca
     1201 cggtgcggag gaaatcggtc caaagattct agagctcctg cagaaatcca atggctttct
     1261 cgccatcaat gataaaactt ctgctgaaaa aatctacgaa ctttttggcg tcagcaaaaa
     1321 gaaatacaaa attgctctgg gcggtcttta caagaaacgt cttatcacgg tgaatgagga
     1381 tggcattcgt cttctagaaa aagaagtgta aagctctgag ttttatcgaa aggattttta
     1441 tttttcggac tccgcctctg cacaaaccac aaatcctcct ctgaacataa caggcttaag
     1501 ggcttccaag gtgctcttcc ggtggtttct tttcgtccgc tctaaacatg gtttttagcg
     1561 aatcaaacag cgccttaaaa tcacgatgac tgacataaac tggtggcggt gctttatcaa
     1621 gagccagaaa tttataaact gcggtttgcg ccgcccgaac agaataatcc accgtaaaaa
     1681 ccacatcatc gggaatttca cagtactggc ccacaaaggc caaatttaaa taagtcggcg
     1741 gaacaacctg cgggcgatct tttttctctc tcgttaaaaa cggactggag acaaagggca
     1801 tcatgcaagg gatacaattg gaacttttta aaatttttgg cagttccagg gtgaaatgca
     1861 gatgcgaaca aagctcgatc ataatctcct ctcctgtaca ttctgacatc ttccttttca
     1921 caaagtttcc tggacgatcc acgaaaaggc cgtagcccca gaaaacattc acccccgagg
     1981 gctgatttat aaaataaggc tgatgagcca acatgagcgt taaaagccaa ttggagtccg
     2041 caaaagtgat gaggccgctg gctcccgcgc tctcccctgt aaattgctcc atcaatttaa
     2101 aaaattctgg atccttaagg gtcaccgtaa aagacaacca cttggattcg tcaacacgat
     2161 tgtcaaacac cgccggaatg ccgaactctg gatgattgac ggccagtgtc tcccacaagg
     2221 tccaggctgc ggactctttt tttgatttca acaggggtgc ggttttcatc gagcccaagc
     2281 tggaggacgc cgtgacagat ccgttggtga caaagacgaa atcggcgggt gctatatcga
     2341 tactattggt gtcttcaccc cgccgataat gaagctttga aatgcttttc tgcactccaa
     2401 tctgagtaaa atcaaagtct gtaacctcgg tttccatcat gaagttcacg cctttagact
     2461 ttagccactt caccaaaggc agtaccaggg agtcatattg attataaggc agacgatcca
     2521 ctcctgccaa aagattgaga cgcgaaaatt cgtggataaa acgatgcaga tatcttttga
     2581 actctatagc gctatgccag gactggaagg caaacagcgt cgcccacata taccaaaagt
     2641 ttgtggaaaa aaaacttttt tcaaaacaat cttctatacg acgacttccc aggctatctt
     2701 cagatttcat cataatctca agtaaatcaa accgatcttt gtagctgaat cccaggatat
     2761 tcgggttcag tttttggcca tttttgacaa gacgacattg agaatgcccc cttgaattga
     2821 cgctgaagtt tgatttcaac ttctcattaa actcccgaat ttcatccaga acagactttt
     2881 taggattttt tatagaaggg ataaaagata ataaatcata agtacactga gtggaaaagc
     2941 tcagcatccg tccccctctc agtacataac ccttttccga gctccccctc ccatcaagac
     3001 tgccgcccac gtccatgtcc tcatcaaaaa taataatatc ctggccgaga aaacgtccct
     3061 cacgcaggag atatgcagcg ctcgccaaag aagcaattcc actgcctaca agatatgctt
     3121 ttctttctac caccggcccc tctccctttc ctgcttttta aaagcctatg tggcatcgac
     3181 tgagagtgta aagtgaagaa acatcgaaaa tatttttgtg agcttgcacg gaatactgtc
     3241 ctcattttcg acaggttttt gtaatttttc atcaactgac ctgttctaaa acaacaagac
     3301 agctcgagag attggcctga tccttgtaat ctttcaaaga gagttcaaaa agatttccat
     3361 gaaacatccc gctgcaatag ttagcggtga acttttactg agtgagcagg ggttttaaag
     3421 atgaaaatat ttttcgcaag tttgattatt ttggggatcc ccaccgaaca agtcgctacc
     3481 gccgccgcag tccatccgac ccagaagttt atcaccagct ttattcaaag cgtgaatgat
     3541 cgcctggaaa ctgtgaacat ggagagagct attgcaggaa atcgtctcta ttgctctcag
     3601 ctttctgacc aacaggtcaa agatatcgaa aatcttttca tttcgaatcc ctcggctaaa
     3661 gcaaatcaat ttacttatcg gagtctcgac aaagtcacga tcggacagtt cattaccgcg
     3721 gccgcagaca acctcggttg ctatcccaca ttctggccga ccaagggtcg ttccacaatt
     3781 ggcggagttt tgtttaattc caaagccttc gttatggacc acttactcct gcaagaagcc
     3841 ctggccgccc tggccggcca cgcgaccgag gacaaccaga ccttgaatac tgtccttcgc
     3901 tagaaagggg tgcaaacaaa ggctttcttg ccctcgactc acgacttggt ctgtaaactg
     3961 gtcttaaatt tgttttcatt ctctcttaaa ttaaaatcat gttatttgcc tcagttctca
     4021 ctcttgtatt tttatcctct tcagcctatg ccaccgacac cgcttcgtta gagggactga
     4081 gtgtcaccga aattggcgcc attccaaaaa attctaaggt ggttcttgaa cctgtagacc
     4141 cttctaagac ttattttcaa tccgccaaca gtggagcaaa tgggagcgag ggttttcaaa
     4201 ctatttcccc ggcggctttt tatagtgtga agtctttctc caattcagca ccttccacac
     4261 ccgacaacgc cacccttcaa tctctggcat ctatgaaaag ccttttaatc caacaagcag
     4321 atctcaccca aaactcccgc aattgcgtcc cctccatcgc aggcctcacc tgcactttgc
     4381 aaaacgaggt gcccgcttac aaatcaggtt tttttgattt tgtcgcctat ttggcaaata
     4441 gaatgaatcc aacgagttgg cttcctccag atcgcacgac tgaagttgac agtaaaccag
     4501 ccagcgatgg ccaagtggtt caggccctag aaaaagcggt tactcaggtg aattcaaagg
     4561 tctcggcgac tcaaaaaagc ctgtctctta actgtgaaac accaccctta ccccccccat
     4621 gcggggttca gcaggctctt caagcttatc atgctcataa aaatgaaata acaaaagata
     4681 tcatcgtttt taacgacttt tccgatggaa agatcatggg aaaaatgtgg cttctcaata
     4741 gtgacggtac acccgcaaaa atcctccaac aaaatcccat tcccgtatcc cggggtccca
     4801 gcggttttgg ctcgggcgac ggatccttga aaactcccaa tggagccctt cttaccaaat
     4861 cttacagacc tccccgggag gggaatgtca aagatggcat tgagcttgta ggacttgatc
     4921 ctgataataa ggatgttcat tctcggggaa tccttctgca cggttggaat ccctatactt
     4981 ccactgaagg gtgcctggga gtggcgggca ccctggaaac cggagtccat ggcaagagaa
     5041 ctgagggttt gccaccttat tatttggacg aattaaaaaa gactgttttc aaagaaggcg
     5101 gagtgctcat ctatgatttc actccgcaaa gaaaaaacct ctgcaaataa ttcccgctat
     5161 gaattctttt tattaacccg gccggaaact ttaggaatgg aaaactttct ttccatgttt
     5221 cgcaaaatct ctgaaacagt tcgagaagct tcatcttttt caagacctac tttctgttgc
     5281 acaatgcctg ccaaggcttc cgcattgccc tcggtttcgc gaatatcatc atcagtgagt
     5341 tgaccccatt ttttcttcaa ctcccctgag atttctttaa attttccttc aaagatgtct
     5401 ctattcataa ctccccccct agccttaagt aaagtaagcc gatatatagt taagacaatc
     5461 ttaatagcgg aagaatgcgg gagtgtgacg taaatttcgc agaaccgtca atgaaaactg
     5521 attctcaaca cttataattt tgcattcaat aaaccaaaac tcacaaaagg agaatccatg
     5581 gaaacaacac ccaatgattt aaaagaaagt gccacttcag caggcaacta tgctaccgaa
     5641 aaagcgcgtc agcttgcgaa tgctgtttca gaaaaaattc aacaacagga attaaaagcc
     5701 gaagaacttt atcgcacagc cagagtcagc gccgaagacg ccttggatac ttctgcggat
     5761 tttgtgaaaa aacacccctt ttccacggtc gctggcgctg ccgctctggg ctttatcgcc
     5821 ggcatattac tccgtcgcac caagcactaa attaaataaa ttataaaagc cctgttctaa
     5881 cagcagggct taatttttta aggagttttc atgcgatggc ttagtctctt aatttcattt
     5941 attattggaa aaatcaacag ttcccgacgc ccgagtctta aagagatggc tttggcaatt
     6001 tttgaggaag cagcttatcg cagccgcagg cctgtcgtct
//
LOCUS       DATBTE010000465        27865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2046192, whole genome shotgun sequence.
ACCESSION   DATBTE010000465 DATBTE010000000
VERSION     DATBTE010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 27865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27865
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2046192"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VIG33_17685"
     CDS             <1..437
                     /locus_tag="VIG33_17685"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY8272730.1"
                     /translation="WSIPEDLKFFRETTRGHIMIMGRKTFDSIGAKPLPQRFHIVITR
                     QNLQSSNALVKYVKTLDEALEAARPLTSQWGDEVFIVGGGEIYKQSMNITDKIYLTVI
                     HQDFEGDTYYPEIPLDRFQLTNKLDRTEPVPFSFLTYTKKIA"
     gene            544..1356
                     /locus_tag="VIG33_17690"
     CDS             544..1356
                     /locus_tag="VIG33_17690"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HEY8272731.1"
                     /translation="MFPLTSMELGKDFELARSLLFGHLPLAVTSKNPAAFLNAYINTY
                     LKEEIQAEGLTRNLPNFARFLQVASFSQASPLVVANVAQEIGIERKIVEDYFSILRDL
                     LLSIELPIFSKRKKRELITKNKFFYFDVGVFRSIRPKGPLDSTAEINGPALETLLLQE
                     IKALNSYLATEYEISYWHTRKHLEVDLILYGPRGFIAIEIKASKRLRPLDFESLKEFR
                     NDYPEDQTYIVYLGAETRNHEGFSVIPAETFIKELPLLLKSGKFKEIEAPDF"
     gene            1419..2843
                     /locus_tag="VIG33_17695"
     CDS             1419..2843
                     /locus_tag="VIG33_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272732.1"
                     /translation="MARLKLWVLILFLAFGFRTPAYDDCNTYLKITETQCIPLGKEMA
                     PNNKAQLYYSIDKIVLLKTLERMTSRAKNLLAIEMGSLEKYIDATRAGATVVSAEYGY
                     SQVLSHTLAICEQFQKKSSEKESEVKNSSPALLLEVSLLSDPQMKPWFDGEADKESIL
                     KKALSPSDSTETKLPTSCPVQLAKNKKFLMDFNIAMTEKKNHLLQRINRYENMEKMIR
                     ESSGDSFAALQSDTEVFLDMFMFTNPSEEPPGKRQRCKYLQVLSALKKSEGFNEAAKE
                     SAANAASLLIGPEALVLGTLGKATASLDKIGNLLHRLNVSGNGNLMPRNLKVMARDTA
                     MMRELGVEADLVRDIPEILYRKYNIGYGEFIERIGLSQTLQRTAAASATKTMDTAYRN
                     LLVQEVQALAKKGVSINPKRLIRATQKELLETRDSAGAYTAEHLVSKGVYPNEPRLLL
                     ERIEREFGGFPQLVQYMGKNFNNF"
     gene            2955..3251
                     /locus_tag="VIG33_17700"
     CDS             2955..3251
                     /locus_tag="VIG33_17700"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY8272733.1"
                     /translation="MNDNTPERHLTQERAYLEIFGRMGTLIAEMKNLSITGAFLEIIQ
                     GDYIPQRGDLLRVTIPLESLGRTHSVNAEVVWGKSLGVGVCFLNHQETMTKMLG"
     gene            3324..4556
                     /locus_tag="VIG33_17705"
     CDS             3324..4556
                     /locus_tag="VIG33_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEY8272734.1"
                     /translation="MKALTYFTAIGFAVLTLIYTQQARTLDKNLYWMKIRARDKFERS
                     VIANTGVSIETTREDFVAATGTLEEKEAIEKLGWLETSFPLTTAMDFPTKDAAFHNYA
                     ELTQKIQELQQTYPNILRVSSAGKSSEGKDMWALRISGNLTQADNLPAIIYLGGHHAR
                     EHLSVELPLYYAEYFLSEYAKGTPRIVNLLNGRDIHILPAVNPDGLEYDVTNESYKLW
                     RKNRSRNGDGTYGVDLNRNYGYQWGTGGSSSNTSSDTYKGPKAFSEPETQAIKAYIEN
                     HQNITTLLSFHTFSELILYPWGHKYQSIEVPRDQQVHQKMAEKMAEWNNYTAEQASAL
                     YQASGDTTDWAYGEHKIISFTFELDPANNGMGSAGFYPGTGVIPVVQQKNLEPVLYLL
                     QYADNPYRVLDVNINPIP"
     gene            complement(4540..6726)
                     /locus_tag="VIG33_17710"
     CDS             complement(4540..6726)
                     /locus_tag="VIG33_17710"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY8272735.1"
                     /translation="MKLNFVDESFMTTGKFRLLALFAAWFFVQSPLFVWAISATDLEA
                     LKVRLKAESEKKSCISSLPLANAVYAEDPNDLYALQTIVKCTRSEQSINQYALHTKEM
                     FERSKILSIVPKLLDVAQVKDLVPILREVEVKKEKSISDYLMINEIYERLGDPEKQID
                     TLKEAIRASPDDPRLLFLLATKQFANQRLGETKGLFKTYLAQAANRPGQIYLMAYVLA
                     LAYPLALSLSLVGLIWALALILGYRKIQVLNDWHDLKLRLPLLVLVVPPLLAFRFWQT
                     GKALPVGALLLFLCIQIFFLLDPLLSKVYRPILRFIGKFFYFVFNGTVLAKKMAELSS
                     GTRFLISFVTLLFLGTIAPTIDIPDLKYSLIILCSMVLYTTIGSLMISFLRSRKSLVV
                     SLRWIGIVATFPFLISYVVSNWSSLGPPLLYGEMPSPVAVDSLVSYLVFWGVSFFLAL
                     HLGKIIAQAFIQPMAEIIEKVSLIEKGQFDAKVKVFSKDEIGDLGHAINRMGSGLEKR
                     EKIEKTFRKYVDHQIAERILDGVENELRIEGQNVDAVVLFADIRGFTSLSEKTSPEEV
                     VKMLNQFFERMVKIIQQNGGVIDKFIGDNVMAVWSVPYPIEDAEKKAITAAIGMLKEM
                     EKWNQELTRQGRAEIGVGIGLNTGKMIAGSIGSADRMEYTVIGDTVNTAQRAESIAKK
                     QQLVVTDAMYEKIKDLVEATPLEPTKVKGKENLQSWWSVTGIKESD"
     gene            complement(6797..8065)
                     /gene="eno"
                     /locus_tag="VIG33_17715"
     CDS             complement(6797..8065)
                     /gene="eno"
                     /locus_tag="VIG33_17715"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEY8272736.1"
                     /translation="MSDIVNIISREILDSRGNPTVEVEVHTALGNIGRAAVPSGASTG
                     AHEACELRDGDKKRFLGKGVFKAVDNVREKLAPEVVGLPVTDQVYVDKVLREVDGTEN
                     KSNLGANAILGVSLAVAKAAALDVKLPLYRYVGGSQACRLPVPLMNLINGGAHANNGL
                     DVQEFMIVPTVNNSYAESLRAGSEIFHTLKSILAKKGLSTAVGDEGGFAPKLASNQEA
                     LDLLMEAIQQAGYEPGQNVFLGLDVAATEMLKEGKYQWQGNLITAKDLQGVYKSWTEK
                     YPLVSIEDGFAEDDWEAWASTTAELGNHVQLVGDDLFVTNPKRLRQGIEKKTANALLV
                     KVNQIGTLTETFEAVSLAQRNKYKTIMSHRSGETEDTTIADLAVALGCQQIKTGSLCR
                     SERVAKYNQLLRIEEDLGGMGIYWDKSAFR"
     gene            complement(8058..9008)
                     /locus_tag="VIG33_17720"
     CDS             complement(8058..9008)
                     /locus_tag="VIG33_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272737.1"
                     /translation="MSVLYEQQTPLPVELMRDPSYWMSAPDMEAFLESLLRMPLKSND
                     GNLLQKVGHEGPEIRAWGVLDSVLRMMPRPQEIFNQPERFLSHFISPEPPVENLRRDE
                     SSISFDLPLPAEQYPHVTTYMKAALESLPVYVGQNLATCEWSGIHLELRWFDQQKSIF
                     EIDPGHQISPQLMQAVIDELQKHQRELEERNCELQKKNEELLQAHMDLQAHLREKAPS
                     LLPQVVPISKLSFEAQGPGYVIGQNTARLHDYMVRAQQLVTMLTAQGKMTPAMKEAMR
                     RVDWEHVKAQYPKTISDIMDSLRGLQNQLVQKTKEENTYV"
     gene            complement(9285..10505)
                     /locus_tag="VIG33_17725"
     CDS             complement(9285..10505)
                     /locus_tag="VIG33_17725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY8272738.1"
                     /translation="MKKPSWINSTVIVVALGYFVDIFDLTLFNMVRVNSLKSLGVMEK
                     DLVDVGVLLLNSQMAGMLLGGLFWGVFGDRKGRLGVLFGSIIIYSLANILNAFVQDIP
                     QYVILRFISGLGLAGELGAGITLIAEILPKEIRGMGTALVAAIGVFGAVLGGIIVELT
                     PWRWTYVIGGLLGLMLVLLRMKVIDSDLFLQKKKNHTEAQWGSLKYLFSDFARVKKFM
                     YCIGIGLPIWFVAGLLMAFSPELAQAVGVQDPITASRTIGISYLGLGIGDFMSGYISQ
                     IFRSRKKTIQIFKALCFAVVCLILFSTAGRGEYYYYTLCFLVGLAAGYWAIFVTTAAE
                     QFGTNLRATVATSVPNFVRGAVIPMTLLFKSLKGGMGIMPAMAIVGLVAFGIATLSAY
                     LLPESFHKDLDYVE"
     gene            complement(10626..12020)
                     /locus_tag="VIG33_17730"
     CDS             complement(10626..12020)
                     /locus_tag="VIG33_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272739.1"
                     /translation="MSILKFLLIMSFIFGWVLQSNAQSIPTVEWYPPETEKSQLPNRS
                     KVVISGRTTGGSLVQIDGDSVTVINEKAPSPTNSNVQPSEAIRQLRANCKVYMAPDIK
                     TKVLASMKKGDDVKAVEFADSWLKVFLKTGPGFMSRPCVVPLKEQAKETSPGSFKIDN
                     RFTRANIEGFFEIAIELPEGRAQIPILVTSPSNAQKTFLVSVNVSINANNNVDDIKMN
                     TKVSQSKPPAAAKKIRLWLGAGLTFQNYGQTMDGAPDLKFQTVQSPGIVARGGYWGDR
                     WGIDFYFRDAPGKIEAEAPLQIQSDTYHWRTAEAKGLYQFARGPSSRIGGRPSQWQLR
                     FGTQLHQIPFLEVSDTNRVTIKESSLQMATLGGGLLLGQEQDLSYEFAVGIQYPLSAS
                     GPGKSFSVSSPFGYEAQIGAAHKFASNWRLGLFSYTQSLAYSYKYENTNGALKNGQQD
                     LFYTTLDLRLGYEF"
     gene            12297..14075
                     /locus_tag="VIG33_17735"
     CDS             12297..14075
                     /locus_tag="VIG33_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272740.1"
                     /translation="MAKEPSLRKKPKSSNRLLQGLIVVTSLLALVFLGEPQLLNQFPI
                     LNGSPSIVNVNTATQASPTNGQKIWLKNQSSVRFEWSGPINEDTFLEISRDRDFKDLV
                     LEEPSPKFPFLTDKLPGDGDYFYRIVQKSSEQENVLLKPVAFTIVTQNPPQLIYPFTP
                     MSIPETKPLRFYWQGKHGVTHYRFQIAFDALFENLFSDLLVEETQTIPQKIPAGHFFW
                     RVRGEDDSTASTLWSEVRVLRSEGVPDIPPVAAAVTLPPPPVTTKAKNPPPAKTLASP
                     KVAKTIQKFMLHYRNKENLRSPASLQNALITPPILSWSKSKGALSYEVQISKKSNFSQ
                     IEWTKSVSAPKTKWDLAKPGKYFWRVQALGETGAKSPFSSLNTLDLSLPAPKIKKLFS
                     HNVKVKSEAQLSEPSPMPISWAEVVAASGYRVVVSENKNFSPTLLDLKVDRNLASVEL
                     NKNGHYFAKVAVLGASGEIVSDYSDIAGLEYEKNYEKKSPSPKVTLPAPQPKLPPNGV
                     SIVSLNESQDPISFKWEATDADAYQLEIASDEKFQQIIHTVVIRENQIVVSKTIPMGR
                     LYWHVRAKKDAYKSDWSPTFSFEFAK"
     gene            14119..14994
                     /locus_tag="VIG33_17740"
     CDS             14119..14994
                     /locus_tag="VIG33_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272741.1"
                     /translation="MNKIILFTFFLAIIGCASEAIKTPSASLFGSWEEPGPSSSNEVI
                     LVPQWHYSPQVNTRGLQVSLPQDKNQKAIFEEMSSWIRSHYIKNLIVEGCEGQLAAAS
                     DLRFNDWSLNDLNGQKNIENAQTHIGLKILSLSKGAINVECGDNLALIKEHQLALSDI
                     RGLAGYKLRLEQANLPLKNRASFVAGAKEALKLPKDTKEDLVLKKLNEELDTVISHFE
                     SLVSKRNDEFVKKARSLAGRKVILIGALHIEDLKSKLNEQKIPFAIWRPIGLENGEVH
                     LIEQLRRQLKLPSHH"
     gene            complement(15049..15237)
                     /locus_tag="VIG33_17745"
     CDS             complement(15049..15237)
                     /locus_tag="VIG33_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272742.1"
                     /translation="MDTLNYEFENIEDREMAYHNIEKWYGSKVVERVDALKISVHASK
                     EIVVATGKIMEKHFGKKN"
     gene            complement(15366..16472)
                     /locus_tag="VIG33_17750"
     CDS             complement(15366..16472)
                     /locus_tag="VIG33_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEY8272743.1"
                     /translation="MSETSSVFKDAIRNNLGPVLPYLEDKTVSEILINGHKEVFVERK
                     GKLERVPASFSDEDALRAAVNSIAQSVGRRINDETPRLDARLPDGSRIAAVIPPCSRK
                     GTIVSIRKFTNNKISFSDYIKFGAITEDGARFLDICMFLGKNILVSGGTGSGKTTLLS
                     LLCTRIPKGQRIIVIEDSSELQVDYEHVVMFETRQGNELGKGEVTIKDLLKSSLRLRP
                     DRIIVGEVRSSEALELLNAMNTGHKGCMGTVHANSPEDAMVRVEALAQGGDAQISEKA
                     LRSQVVSAIEIIIQVSRFSDGSRRIAAISEVMGILPEGNYDVRPIFQMSRMTRRPDGT
                     LEGKLQSTGYIPSFMEEIVDNKLPFPQSKFNKVA"
     gene            complement(16484..16876)
                     /locus_tag="VIG33_17755"
     CDS             complement(16484..16876)
                     /locus_tag="VIG33_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY8272744.1"
                     /translation="MSRRQLDFNRVTNQNRLPDSTKPDETQNLFGQVFDSIEPTQDIV
                     ILNTEKGFMPDTLRVKKGNSYRIHVVNVNSKDKNVSFVLDAFSEHHNTLYGQERVFNL
                     NPKVEGVFSYQCPETAVQGRIIVISASN"
     gene            complement(16929..17360)
                     /locus_tag="VIG33_17760"
     CDS             complement(16929..17360)
                     /locus_tag="VIG33_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272745.1"
                     /translation="MTAPSQKAGISPAFSIFNIFIPLRLNLTQVLRALADKSFRFQDG
                     FWKERLGMFLQGDLQTVFDALYTIGAIDPVLKLDWEQVLNEMAQNPEMIVHAMDVINA
                     CDGDKDLMVQRLHMMDVKVVNYIAMEVAREFVEFQDRSCLH"
     gene            complement(17376..17990)
                     /locus_tag="VIG33_17765"
     CDS             complement(17376..17990)
                     /locus_tag="VIG33_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272746.1"
                     /translation="MKKYLFILASLLWASVSSAGLLYNYSQLTLKDLDQMNALAQKKI
                     KEFKKDGSIEILKEAVQAIYSRPNDDGMVEKVIPPLRHELDENEQWETTMDALVQEAV
                     GALKNPKAFKPVVQNTYAIFLENVISDFKPFAEREGHERKTLKLIADADIELSKEAVN
                     ERKLRTMSSHKSPSEIAALVMKNIAKAEADNKKVEEDKKKKEKK"
     gene            18185..18982
                     /locus_tag="VIG33_17770"
     CDS             18185..18982
                     /locus_tag="VIG33_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEY8272747.1"
                     /translation="MNKSALIALSIMAFALTNCAPSAKQIKEAVEKDPSIVFVAIEKY
                     PDQFIEVVNKAAQDAQKKSQDKAMAEESKKRDEEFANPLKPEVQPDRVIFGNKDAKVT
                     IIEYSDFECPYCSKGFQTVKQVLKEYPNDVRVVFKHLPLDFHPQAMPAAKYFEAVAKQ
                     SPEKAEKFHDAVFENQGELRSKGEDFLKNTAKKLGVDMKKLDKDVKDESIMTRIHADM
                     EEAKKFNFSGTPGFLINGVSLRGAYPFPEFKTVIDRHLGKSVETKKQ"
     gene            complement(19090..19824)
                     /gene="sfsA"
                     /locus_tag="VIG33_17775"
     CDS             complement(19090..19824)
                     /gene="sfsA"
                     /locus_tag="VIG33_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEY8272748.1"
                     /translation="MKYRMQLDEGVFLKRYKRFFADIEWKGQILTAHVPNTGSMKSVN
                     HPGQACLFSHSDNPERKLKQTLEMIQAPTGVWVGVNTATPNMVVRETLERVVGNAQEK
                     WPKTWQHWTSFDVVKPEFKISAETRLDFALMRNASAKKHFIEVKNVTLAEEKTAMFPD
                     AETTRGQKHLQELISLMREGHSAEILFTIQRNDCDKFSSADEIDPEYGRLLREAHKKG
                     LRISPFVVDLNPYEVVLSDRLLPLNF"
     gene            19884..20810
                     /locus_tag="VIG33_17780"
     CDS             19884..20810
                     /locus_tag="VIG33_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEY8272749.1"
                     /translation="MRRFEHQWQEFKWIDIEGPSQEDFVHLANEFELPLRPLANCLDP
                     EHLPRCEFFDSMAFFILRHFDTTAKESAGTMQDLTTKLVFVVGKDYVLTIHRASIHSI
                     NDEIRKCDFENLSLVNFMQSLCSQVIESFDSPIENLEAKTEIIEERVFALRRRNILRE
                     GYRIKRKTSAYRKLFKFTLDVLVKLQTRPDFPLNDISDIRESLDRLIFYVDNIYEDIT
                     GLLNLHLSLMSQKTNEASFKTSEIMRILTVVSIFFLPLNFIAGVYGMNFENMPELKNA
                     NGYHFALGAMLLIALAILLWIYKKGWLKKEDL"
     gene            complement(20833..21834)
                     /locus_tag="VIG33_17785"
     CDS             complement(20833..21834)
                     /locus_tag="VIG33_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272750.1"
                     /translation="MIKLALLLSLSLLSISSVSCWAGGGDFVNNGGGLAEKNILYAYE
                     KMDTYIQLCLKAESCKLNATQRALLEKIYQALPQEKQEQQIFFGSERKTPGFFIIEGN
                     VRVAKTGDKVGSPIQVNADLLYSKNETGSYDPVTIPEAVAILIHELGHHHGEHSHEEL
                     DLIGVRVSLLLQQKIISTPLVPWAPSEISASVLNSDLLNSFPEVLLNVGEDVMDVSRI
                     YAKEVHCEVLSLPIPIFPVPDLELMTRIPAGSLFHNVHWEKIKDKGTYIEVRITGNVS
                     NNCKYKNNIGIRNNNYQLSISFKVNKVSDRWVLDPASIALLQFTDPWWKVLRIPSSP"
     gene            complement(21827..22666)
                     /locus_tag="VIG33_17790"
     CDS             complement(21827..22666)
                     /locus_tag="VIG33_17790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HEY8272751.1"
                     /translation="MIDRNSLFRSLAFGIAQDISVPMELTIKKLLMQELTTRQARNPS
                     YSIRAFARDLEIGVATLSEVLGGKRNISKTNLEKVLEKLLVSPLEREKLWDEYRDKVN
                     RPHEVDDKMLIDEDLFRLIADWHYLAVLNLSKVKGFNGNPEWIAQRLGIKASEAKESL
                     ERLMRLELFRKVRGRYVRTKKSLTTTRDIPSAAIRKHHSQNLLLAEQSLHRDPVEYRE
                     FGSVTLAVNLKKLPLAKELLLKTRKKIVDMLDKGETSEVYTLSFQLFPLTKLQTLMEN
                     NDD"
     gene            22794..23360
                     /locus_tag="VIG33_17795"
     CDS             22794..23360
                     /locus_tag="VIG33_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272752.1"
                     /translation="MKKIILAIAMVLGVSQAHAHSVPAWVCSLSFKGEAEGFKLIYGD
                     YAFNGKGDLLCANPAGKSVAYPVIVTMNSKKLSPQVSFGRMRLSGQAVEFSLFNHNPE
                     DVLGTYYIAQGQAAVVGGIGAITATKVHLPEIALHISLQFARGLGVNLGLNKMVIALD
                     SNEAPAPTPVPEEPIAPAPAPAPAPTPY"
     gene            23577..25133
                     /locus_tag="VIG33_17800"
     CDS             23577..25133
                     /locus_tag="VIG33_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEY8272753.1"
                     /translation="MATDIKLPELGEGVSEGELVKWLVKPGDSIKTDQPIAEVMTDKA
                     TVEVPTPTAGIVKELKFKPGQVVKVDAVMITLDAAGVSASTPPKSNPTAAATPTTSTT
                     SASTPVKLPELGEGVSEGELVKWLVKPGDAVKADQPIAEVMTDKATVEVPTPLAGTVK
                     ELKFKTGDVVKVDSILLILEGGAAPKISPQISTPTPASVSYAATSASIHPPVADSKVL
                     ATPATRRLAREMNVDINALNGTGLAGRVTREDVFSMGAGATRAGAAVPAQAPGLNIIP
                     RPAYQGGTGAEERVALIGIRRKIAENMQKSKAIIPHFTIMDQANVTELVALRESLKDF
                     AEKNGTKITYLPIVIKAMIATMREFPMFNASIDDAASEIVYKKYFNIGFAADTPNGLV
                     VPVIKNAEQKTIIELSKEILDLSKRARDGKLKVEEMKGACITVTNIGSIGGTYATPVI
                     NHPEVAIVGMYKIDEKPVIKNGQLAAIKVMNYTVTADHRLIDGAVAAKFLKSFLERIE
                     NPARLMMDMI"
     gene            25142..26584
                     /gene="lpdA"
                     /locus_tag="VIG33_17805"
     CDS             25142..26584
                     /gene="lpdA"
                     /locus_tag="VIG33_17805"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEY8272754.1"
                     /translation="MQNFDVVVIGSGPGGYVAAIRSAQLGFKTAVIEREFLGGVCLNV
                     GCIPSKAMITATHLLHKAQHNFPTMGLKIKGAIEVDMKQLVAWKQSVCDKMSGGVNQL
                     LKGNSVVILKGEAEFKNSKELSLKSSSGVDSIQAKYFVVATGSRPIEIPAFKFDEKDV
                     VSSTGALAWNEIPKRVVTIGGGYIGLEISSYLRKLGCEVTVVEAQSSLLSGVVDPECA
                     QIVTRKLEKSGVKVLYGAKAKGQKKNKDGYEVTVEINGKDEVIKADKILVTVGRRPNG
                     DQANLKAAGIVVDERGFIKVDAQRRTSVPHIFAIGDICGQPMLAHKASHEGVLVAEVI
                     KGYNRVYDAKTVPAVVFTDPEIAAAGLTEAEAKAKGFNNLKVAKFPFAANGRAVSMME
                     TEGFVKMIADAKTNVLLGVHIVGPEASNLISEAVLAIEMGARIEDLALSIHPHPTLGE
                     AMMECAEATLGHAIHIIQKPLTGNGKNAHA"
     gene            complement(26644..27773)
                     /locus_tag="VIG33_17810"
                     /pseudo
     CDS             complement(26644..27773)
                     /locus_tag="VIG33_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_034400331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         8032 a         6480 c         5777 g         7576 t
ORIGIN      
        1 cctggagcat tcccgaggat ttaaaattct tccgagagac caccaggggc cacatcatga
       61 tcatgggccg taagaccttt gactccattg gcgccaaacc cttgccacag agatttcaca
      121 tcgtgatcac ccgccagaac ctgcagtcct caaatgcact ggtaaaatat gtgaaaactc
      181 tcgatgaggc cctcgaagcc gcacgacctt tgacttcaca atggggtgac gaggtgttca
      241 tcgtcggtgg tggggaaatc tacaaacagt cgatgaatat caccgataaa atttatttga
      301 cggtgattca tcaagacttc gaaggggaca catattatcc tgaaattcct ttggacagat
      361 ttcagctgac gaataagttg gaccgcactg agccagtgcc tttttctttt cttacttata
      421 caaaaaagat tgcataagtc cattttttcc acaaatatgg acttatgtac tccagaatta
      481 tttcacccca aaaacgtcgt cgtttttttt attcgggcct cgaggaacgc gctaacgctt
      541 gaaatgtttc ctctcacaag catggaactt ggcaaagatt ttgaacttgc aagaagtctt
      601 ctctttggac accttccctt ggccgtaaca tctaaaaatc cggcagcctt tttaaatgct
      661 tatataaaca cctatttgaa agaggaaatt caagcggagg gtcttacccg taatcttcct
      721 aatttcgctc ggtttttaca agttgctagc ttttctcagg caagtccttt agtcgttgcc
      781 aatgttgccc aggaaattgg aatcgaaagg aagatagtag aagattattt ttcgattttg
      841 cgcgatcttt tgctctcaat tgaacttcca atcttcagta aacgcaaaaa gcgcgaactc
      901 atcacaaaga ataagttttt ttattttgac gtgggtgtct ttcgttcgat tcgccccaaa
      961 ggccctcttg attctacagc agaaataaat ggaccagccc ttgaaaccct tttgctgcaa
     1021 gaaatcaaag ctctgaactc ctacttagcc accgaatatg aaatcagcta ttggcacaca
     1081 cgaaaacatt tggaagttga cctcatactt tatggacccc gtggttttat agccattgaa
     1141 ataaaagcca gtaagcgctt acgacctttg gattttgagt ctctaaaaga gtttcgaaat
     1201 gactaccctg aagatcaaac atatatagtt tatctaggcg ccgagaccag gaatcatgaa
     1261 ggattttcag ttatccccgc agagactttt attaaggaat taccacttct actcaagtcg
     1321 ggaaaattta aggaaataga ggctccagac ttttaatttg ctgatttctc gatataatta
     1381 agccagatcc tcctcaatcc ttgatttcag gagataattt ggctcgctta aaattatggg
     1441 ttcttatttt atttctggct ttcggtttca gaacgcctgc ttacgatgac tgtaatacct
     1501 atttaaaaat aaccgaaact caatgcattc ccttgggcaa agagatggct cccaataaca
     1561 aagcgcagct ttattatagc atcgataaaa tcgttctcct taaaactctg gagcgaatga
     1621 cttctcgagc gaaaaactta ttggcaatcg agatgggctc ccttgaaaaa tacatcgatg
     1681 ccacaagagc gggcgctacg gtcgtgagtg cggaatatgg ttattcgcag gtgctgagcc
     1741 atactttggc catctgtgaa caatttcaga aaaaatccag tgaaaaagaa tcagaagtaa
     1801 aaaacagttc cccagcgttg ttgctagaag tttccctgct ctctgatcca caaatgaaac
     1861 cttggtttga tggagaggcc gacaaggagt cgattctaaa gaaggctctg agtccttcgg
     1921 attcaactga gacaaagctt ccgacttcgt gccctgtcca attggcgaaa aataagaaat
     1981 tcctgatgga cttcaatatc gcgatgactg agaagaaaaa tcatctgctt cagagaatca
     2041 accgctatga gaatatggaa aaaatgattc gtgagtcttc gggcgattca ttcgcagcgc
     2101 ttcaatccga tactgaagtc tttcttgata tgtttatgtt taccaatccc agcgaagaac
     2161 ctcccgggaa aagacaaaga tgcaaatatt tgcaagtgct cagcgccctc aagaaaagcg
     2221 aaggtttcaa tgaggcggcc aaagaaagtg cagctaacgc cgcaagttta ttaatagggc
     2281 cggaagcctt agtgcttgga actctgggaa aagcgaccgc cagcctcgac aagattggga
     2341 acttattgca taggttaaat gtaagtggca atggcaacct catgccgaga aatcttaaag
     2401 tgatggccag agacacggcg atgatgagag aactcggtgt ggaagcggat ctggttcgtg
     2461 atattccaga gattctctat cgcaaatata atattggcta cggcgaattt attgaaagaa
     2521 tcggcttgtc ccagacccta cagcgaactg ccgctgcctc ggccacaaaa acaatggaca
     2581 ccgcctatcg caatctcttg gtgcaggaag ttcaagctct cgcaaaaaaa ggcgtgagta
     2641 tcaaccccaa acgtttgatt cgagcgaccc aaaaagaact cttggaaacc cgggacagtg
     2701 caggggccta tactgctgaa cacttggtat ctaaaggagt ctaccccaat gagcctcgcc
     2761 tgctgcttga acgcatcgaa cgagagtttg ggggctttcc tcagttggtg caatacatgg
     2821 gcaaaaactt caacaatttc taaaagattt ttttccatca atctcatgat gagaatgtgc
     2881 atcggccctt cggaatagac ttagaatctc ggaacttatg accgatacta agtctataaa
     2941 aaggaagaaa tataatgaac gataatacac cggagcgaca tctaacccaa gagcgagctt
     3001 atttggaaat ctttgggcgg atggggactc tcattgcaga aatgaaaaac ctgtctatca
     3061 ccggcgcttt cttagaaatt atccagggtg attatattcc tcagcgtgga gaccttttgc
     3121 gggtcactat tccgctggaa tctcttggac gcactcatag tgtgaatgcg gaggtcgtct
     3181 gggggaaaag cctcggcgtt ggcgtgtgct ttttgaatca ccaagaaacc atgaccaaaa
     3241 tgctggggtg aaacatagac catagtcttg ttgtgccttt attttagtcc atgctagcct
     3301 taagtcactt taaggggggg accatgaagg ctctaacgta ttttacagca attggatttg
     3361 cagttttaac acttatttac actcagcaag ctcgcactct cgataaaaat ctgtattgga
     3421 tgaaaatccg ggctcgcgat aagtttgagc gcagtgtcat tgccaacact ggggtttcta
     3481 ttgagaccac gcgcgaagat tttgtggcag cgacaggtac attggaagaa aaagaagcta
     3541 tagagaagct gggctggctc gaaaccagtt ttccgctcac taccgctatg gactttccga
     3601 cgaaggatgc ggcttttcat aattacgcgg aactcactca gaagattcag gaactccagc
     3661 aaacctatcc gaacattcta cgggtctctt ccgccgggaa gtcctctgaa ggaaaagata
     3721 tgtgggcttt acgcatttct ggaaacctga cgcaagctga caacttgcca gcaattatct
     3781 atttaggcgg acaccatgcc cgcgaacatc tttcggtgga attgccactt tattatgctg
     3841 agtatttcct cagtgaatat gcaaaaggca cacctcgtat cgtcaatctt ctcaatggcc
     3901 gtgatattca tatccttccg gcggtcaatc cagatggact tgaatatgat gtgaccaatg
     3961 agagctataa gctctggcgt aaaaatcgct cgcgcaatgg cgatggcacc tatggggtgg
     4021 atctgaatcg caactatggc taccaatggg gaacaggcgg ttcgagcagc aacaccagca
     4081 gtgataccta caaaggacct aaagctttca gcgaaccgga aactcaagct attaaagctt
     4141 atattgagaa tcaccaaaac atcacgactc ttttaagctt ccataccttc tcggaattga
     4201 tcctttatcc ttggggccat aaatatcagt ccattgaagt ccctcgcgat caacaggttc
     4261 atcaaaaaat ggcggagaaa atggcggaat ggaataacta tactgcagaa caagcttcag
     4321 ccctttatca agccagtggt gacaccacgg attgggctta cggtgaacat aagattattt
     4381 ctttcacttt tgaattagat cccgcgaaca acggtatggg cagcgccggc ttctatccgg
     4441 gaactggagt gatcccagtg gttcaacaaa agaatttaga accggttttg tatcttctcc
     4501 agtatgccga caatccttat cgtgtgctgg atgtgaatat taatccgatt ccttaatgcc
     4561 agtaacggac caccaacttt gaaggttttc ttttcccttc actttggtgg gttccaatgg
     4621 tgttgcttca accaaatctt ttattttttc atacatggca tcggtcacta ccaactgctg
     4681 ttttttggct atggattcgg ctctttgagc agtattgacg gtgtctccta taaccgtgta
     4741 ttccatgcga tctgcacttc cgatactgcc cgctatcatc ttgccggtgt taagaccaat
     4801 acctactccg atttccgccc gaccttgacg agtgagctct tgattccact tttccatttc
     4861 tttaagcatg cctattgcgg cggtgatggc ctttttctcg gcgtcttcaa tagggtacgg
     4921 aacactccag accgccatca cgttatcccc tatgaatttg tcaatgaccc cgccattttg
     4981 ttgaatgatt tttaccatgc gctcaaaaaa ctgattgagc attttgacga cttcctcggg
     5041 ggaggtcttt tctgacaggg aagtgaatcc ccggatatcg gcaaacaaaa ccacggcatc
     5101 gacgttttga ccttcaatgc gcagctcatt ttcaacgccg tctaaaattc tttctgcgat
     5161 ttggtggtca acatattttc ggaaagtctt ttcaattttt tcccgttttt cgagaccgct
     5221 acccatacgg ttgatcgcat gacctaagtc gccgatttcg tctttcgaaa aaactttcac
     5281 tttggcatca aattgcccct tttcaattaa agagaccttt tcaataattt cagccatggg
     5341 ctgaataaag gcttgggcga taattttgcc taagtggaga gccaagaaaa aggaaactcc
     5401 ccagaacacc aaatagctca ccagactatc gacggctaca ggagaaggca tttctccata
     5461 taacagcggc ggccctaaac tgctccagtt tgaaaccaca taagaaatta gaaatggaaa
     5521 agtcgccaca atgccaatcc atcgcaacga aaccaccaga cttttgcgag aacgcagaaa
     5581 gctgatcata agtgagccga tggtcgtata taaaaccata gagcaaagaa taatcagact
     5641 atatttaaga tcgggaatat caattgtcgg cgcaatggtt cccagaaata atagagtcac
     5701 gaaggaaatt aaaaatcgtg tgccagatga aagctccgcc atcttttttg cgagcaccgt
     5761 cccgttaaag acaaagtaaa aaaacttgcc gataaatctc agaatcggcc tgtaaacttt
     5821 ggaaagcaaa ggatcgagaa gaaaaaatat ctgaatacaa agaaacagca gcaaggctcc
     5881 gacgggcaaa gcttttccgg tctgccaaaa acgaaaagcc aataagggtg gaaccacaag
     5941 caccaagagc ggtagacgca atttgaggtc atgccaatca ttgagtactt ggattttccg
     6001 ataacccaaa atcaaggcca aagcccaaat cagtcctact aagcttaaac tcaaagccaa
     6061 tggataggcg agcgctaaca cataagccat caagtaaatt tgacctggtc tattcgcggc
     6121 ttgcgcaaga taggttttaa agaggccttt agtttctcct agacgctggt tagcaaactg
     6181 tttcgtggcc aaaaggaaaa gtaggcgagg atcgtctggc gacgctcgaa tcgcctcttt
     6241 taatgtatca atttgtttct ctgggtcccc aaggcgttca tagatttcgt tgatcatgag
     6301 gtaatccgag atactttttt cttttttgac ttccacttct cgtagaattg gaactaggtc
     6361 tttaacttgg gccacatcca aaagcttcgg cactatggaa agaatttttg agcgctcaaa
     6421 catttcttta gtatgcaagg catattgatt aatactctgc tctgatcgtg tacatttgac
     6481 aatcgtctgc agggcataaa gatcattcgg atcttcagcg taaacggcgt ttgcgagagg
     6541 caatgaagag atacaggatt ttttttcaga ctcggccttg agacggactt ttagagcctc
     6601 taggtcagtc gcagaaatgg cccaaacaaa gagaggactc tgcacgaaaa accaggccgc
     6661 aaataatgcc aataaacgaa actttccagt cgtcatgaag ctctcatcga caaaattcaa
     6721 tttcacacaa gagctgcact ggaaaactcg acgtagtcca ggcattcaac ctggacttat
     6781 gaacggaagc aaaactttag cggaacgcgg acttatccca gtagattccc atcccaccca
     6841 gatcttcttc gatgcgtaaa agttggttat acttcgccac ccgttcagac cgacacaagc
     6901 tgccggtttt gatttgctga caacctaaag ccaccgccag atccgcgatc gtggtatctt
     6961 cggtttcgcc cgagcgatgg gacatgattg tcttatattt atttctctga gccaggctaa
     7021 cagcttcaaa ggtctctgtg agagtcccga tttgatttac cttaaccagc aaggcattgg
     7081 cggttttctt ttcaattccc tggcgcaggc gttttggatt ggtcacgaac aaatcatccc
     7141 ctaccaattg aacatgattg cccagttctg cggtggtgga agcccaagcc tcccaatcat
     7201 cctctgcaaa gccgtcttcg atactcacga gcggatattt ttcagtccaa gatttgtaaa
     7261 ccccttgaag gtctttggca gtgatgaggt tgccttgcca ttgatatttg ccctctttca
     7321 acatctcagt cgccgcaaca tccagaccta agaaaacatt ttgacccggc tcataacctg
     7381 cttgttgaat ggcttccatc aacaaatcca aagcctcttg attgctcgcc aatttcggag
     7441 caaagccgcc ttcatccccg acggcggtcg aaagtccctt tttagccagg atggatttca
     7501 acgtatggaa aatttcagag cccgcacgaa gcgattccgc gtaagagtta ttcacggtag
     7561 ggacaatcat gaactcttgc acatcaagac cattgttggc atgagcacca ccgttgatga
     7621 ggttcatcaa cggaaccggc aagcggcaag cctgagagcc cccaacatag cgatacaaag
     7681 gaagcttcac atccaaagcc gcggcttttg ccaccgctaa agaaacaccg aggatcgcat
     7741 tcgcccccaa gttagatttg ttttcagtgc catcaacttc gcgcaaaact ttatcgacat
     7801 agacttgatc agtgactggt aaacccacca cttcaggagc caatttttca cgaacattat
     7861 ccaccgcttt aaaaacacct tttcctaaaa agcgtttttt atctccgtcc cgcagctcgc
     7921 aggcttcatg cgctcccgtt gaggctccgg atggaacggc ggcgcgaccg atattaccaa
     7981 gagcggtatg aacttccact tccacagtag gatttccacg actatccaaa atttcacgag
     8041 agataatatt cacaatgtca gacataggtg ttttcctctt tggttttttg aacaagttga
     8101 ttttgcaaac ccctcaggga gtccatgata tctgagatgg tctttggata ttgagctttg
     8161 acatgttccc aatccactcg gcgcatagcc tctttcatcg caggagtcat tttcccctga
     8221 gccgtcagca tcgtcactag ttgctgagct cgcaccatat aatcatgcag gcgcgccgtg
     8281 ttttgtccta tgacatatcc gggtccctga gcttcaaaac ttaatttcga aatcggcacc
     8341 acctgcggaa gaagagacgg ggctttctca cgtaaatgcg cctgcaaatc catatgagct
     8401 tgtaagagtt cttcgttttt cttttgtaat tcacaattgc gttcttcaag ctcacgttga
     8461 tgtttttgca attcgtcgat cactgcttgc attaattgag gactgatttg atggcccgga
     8521 tcgatttcga aaatggattt ttgttgatcg aaccaacgaa gttcaagatg aattccagac
     8581 cactcgcagg tcgccaggtt ttgccccaca tacaccggca aagattcaag tgccgctttc
     8641 atataggttg tcacatgagg atattgttcc gcaggcaaag gcaaatcgaa ggagatcgaa
     8701 ctttcatctc tgcgcagatt ttccactggt ggctcaggag aaataaaatg tgacagaaaa
     8761 cgttccggtt ggttaaaaat ttcttgaggt ctcggcatca ttctcaacac cgaatcaaga
     8821 actccccaag cccgaatctc gggtccttca tggcccacct tctgtaaaag attgccgtca
     8881 ttgctcttta aaggcatgcg gagcaaagac tctaaaaaag cttccatatc cggtgcactc
     8941 atccaatagg aggggtcccg catcaactcc acaggcaaag gagtttgctg ttcgtacaaa
     9001 acggacaaat cctcgccctg ggtctccagg taggtcaaaa tagagttaga gatcttacaa
     9061 ctgaagtgca ctaaaaactt gtagcccaaa agaaattaga tgggaagaag gtacggccta
     9121 ctttttagta ttgcgtcgca ttaacttgag cttttgctta agtttataac attcgcgaat
     9181 tccgtccatc ataagtcaca acttgatctc ttgacttgct cagtcactaa atcgtttttt
     9241 actgaggcga tggtaaacga gattaaacaa aaaatatcca ttttttattc aacataatcc
     9301 agatccttat gaaagctttc agggagcagg taagctgaga gggtcgcaat accaaaggcg
     9361 accaatccca caatcgccat cgctggcata attcccattc cacccttaag ggatttaaat
     9421 aacaatgtca taggaatgac cgcaccccgt acaaaattcg ggacgctggt agccacggtc
     9481 gccctgaggt tcgtgccaaa ttgttcggcg gcggttgtta caaagatagc ccagtagcca
     9541 gcagcaaggc caacgaggaa gcacagagtg taataatagt attctccccg tccggctgtg
     9601 gaaaataaaa tcaggcatac cactgcaaaa caaagagctt taaagatctg aatcgttttt
     9661 ttacggctcc taaagatttg actgatataa ccactcataa aatcgccgat gcctaagccc
     9721 aaataactga taccaatggt tcttgaggca gtaatgggat cttgtacgcc aacagcttgc
     9781 gcaagttccg gagaaaaagc catcaaaagc ccggcgacaa accaaatagg gaggccaatg
     9841 ccgatacaat acataaactt ttttactcta gcgaagtcag agaaaagata tttcaggctg
     9901 ccccattgcg cttctgtatg atttttcttt ttttgcaaaa aaagatctga atcaatcact
     9961 ttcattctga gtaaaaccaa catcaatccc agaaggccgc cgataacata ggtccaccgc
    10021 caaggagtca gttccacaat aatcccgcca aggaccgcgc caaaaacccc aatggctgcg
    10081 accagcgcag ttcccattcc gcggatttct ttaggcaata tttcagcaat aagcgtgatg
    10141 ccagcaccaa gttcgccagc aagccctaat ccagatatga atcttagaat aacatattga
    10201 ggaatatctt gcacaaaagc atttaaaata ttggctaaag aataaataat gatcgagccg
    10261 aaaagaaccc ctaaacgacc tttgcggtca ccaaacactc cccaaaataa tccgcccaaa
    10321 agcatccccg ccatttgcga attaagaaga agaaccccga catcaacgag atccttttcc
    10381 atcacgccga ggctttttaa ggaattgacc cgaaccatgt tgaataaagt gagatcaaaa
    10441 atatcaacaa aatatccaag ggccacaaca ataacggtcg agtttatcca agacggcttt
    10501 ttcatgggag gcatttgatc actgaaactg ccttggagca agggatagta gagcccttta
    10561 tggtcggcta ggaaccacag acaagcactt aaaatatgac attttaagac agtgcgaatt
    10621 tatcttcaga actcataacc aagtcttaga tccaaggtgg tgtaaaaaag gtcctgttgc
    10681 ccatttttca aagcgccatt agtgttttca tatttatagg aatatgccag cgactgggta
    10741 taagaaaata aaccaagacg ccaattggag gcaaatttat gggccgctcc tatttgagcc
    10801 tcatagccaa aaggtgacga gacagaaaat gattttcctg gtcctgaagc tgaaagtgga
    10861 tattgaattc ccacagcaaa ctcataggat agatcctgtt cctgacctaa gaggagacct
    10921 ccccccaggg tggccatttg caaactgctt tcttttattg tcaccctatt ggtatcactg
    10981 acttctaaaa aaggaatttg atgaagttgg gtgccaaagc gaagctgcca ttgacttggg
    11041 cgccccccga tgcggctgct aggacctcgg gcaaattgat agagtccttt ggcttcagcc
    11101 gttcgccaat gataagtatc agattgaatc tgcaggggcg cttctgcttc gatttttccg
    11161 ggggcatccc taaaatagaa atctataccc cagcgatctc cccaatagcc tccgcgagcg
    11221 acaatgccgg gagactggac agtctgaaat ttaagatcag gtgctccatc catagtttgg
    11281 ccataatttt gaaaagtgag gccggcccct aaccagagtc gaattttttt tgcggcagcc
    11341 ggaggtttgc tttgagaaac tttcgtattc attttgatat catctacatt attattggca
    11401 ttaatgctca cattcaccga gaccaggaat gttttctgcg cattcgaagg actggtcacc
    11461 aaaatcggga tctgtgcacg cccttcagga agctctattg caatctcaaa gaagccctcg
    11521 atatttgcac gagtaaatcg gttatctatt ttaaaacttc ccggagaagt ctctttggct
    11581 tgttccttca gcggaacaac gcatggccgc gacatgaatc ctggacctgt tttcaagaaa
    11641 acttttagcc aactgtccgc gaactccaca gcttttacgt catcgccttt tttcatggaa
    11701 gccagaacct tcgtcttaat atctggagcc atataaactt tgcagtttgc ccgcaattgc
    11761 cgaatagctt cagaaggctg aacgttacta tttgtaggtg atggcgcttt ttcatttata
    11821 accgtgacac tatcgccatc aatttgcacc agagagccgc cggtggtgcg ccctgaaata
    11881 accactttcg aacgattcgg aagctgactt ttttctgtct cgggaggata ccactcaacc
    11941 gtgggaatag actgagcatt ggattggaga acccatccaa aaataaaact cattattaaa
    12001 agaaatttca aaatactcac ccgtttgaac tccaccgtgc attattctgt acacacttca
    12061 ctttgtagac atatcggaga aaaattcaat aacctaaaca attcttatct taaaacccca
    12121 gataaaacct aagtctagga aaatagaact taagcctaaa aagaccacag gcctccttaa
    12181 aaaaaccagt ggaatggtct agttctctaa aatttctaaa tccagttcta tagattcttt
    12241 ataacagccg atatagaaac agaagataaa ataagaccat gagaataaag gtttaaatgg
    12301 ctaaggaacc gtctttacgt aaaaaaccaa agtcatcaaa tagattgcta caaggattga
    12361 ttgtagtgac gtctctattg gcgcttgttt ttcttggcga acctcaattg ctaaatcaat
    12421 ttccaattct aaatggaagc ccgtcaattg taaatgttaa cactgcaact caagcctcac
    12481 caacaaacgg tcaaaaaatt tggttaaaaa accagtcctc ggtgcgtttt gaatggtctg
    12541 gacccattaa cgaagatacg tttttagaaa tttcgaggga tcgggatttt aaggacttgg
    12601 ttcttgaaga gccttctcct aaatttccat ttcttaccga taaacttcca ggcgatggag
    12661 attattttta cagaatcgtt caaaaatcct cggaacagga aaatgtgttg ctcaagcctg
    12721 tcgccttcac aatcgtaact caaaatccgc ctcaattgat ttatccattt actcccatgt
    12781 ccattcctga aaccaaaccc ctgcgatttt attggcaagg aaagcatggg gtgactcact
    12841 atcgatttca gatcgccttc gacgcactct ttgaaaatct tttcagcgat ctcctcgtcg
    12901 aggaaactca aactattcct cagaaaatac ccgcaggtca ttttttctgg cgtgttcgcg
    12961 gtgaagatga ctctacagcc tctactctgt ggagcgaagt ccgtgtcttg cgttctgaag
    13021 gtgtcccgga catcccgcct gtcgcagcgg ctgttacatt gcctcctcct ccggtgacaa
    13081 caaaagccaa gaatcctcct ccagcaaaaa ctttggcgag ccccaaagtg gccaaaacaa
    13141 ttcaaaaatt tatgctgcat taccgtaaca aagaaaatct tagaagtccg gcctctctac
    13201 aaaatgcctt aatcaccccc cccattttga gttggagtaa gtcaaaagga gctctttctt
    13261 acgaagtaca aatttcaaaa aaaagcaatt tttcgcagat agagtggacc aagagtgttt
    13321 ccgccccgaa aacaaaatgg gatctggcaa agcctggaaa atatttttgg cgagttcaag
    13381 ccttgggcga aactggtgcc aagagtcctt ttagttcctt aaatacactt gatctcagct
    13441 taccggcacc aaaaattaag aaattatttt cgcacaatgt gaaagtgaaa tcagaggcac
    13501 aacttagcga gccctcgccc atgccgattt cttgggcaga ggttgtcgcg gcttcagggt
    13561 accgtgtcgt ggtttcagaa aataaaaact ttagccccac cctgcttgac cttaaagtgg
    13621 atcgaaatct tgccagtgtg gagctcaata aaaatggaca ctatttcgca aaagtagcgg
    13681 ttttgggagc cagcggagag atcgtcagtg actattcgga cattgcgggg cttgaatatg
    13741 aaaaaaacta tgaaaagaag agtccttctc caaaagtaac tctccctgca cctcagccaa
    13801 agcttccacc caatggagtt tcaattgttt ccttgaatga atctcaagat cccatttcat
    13861 ttaaatggga ggcaaccgat gccgatgctt accagttgga aattgcctcg gatgaaaagt
    13921 tccaacaaat tattcacact gtagtgattc gggaaaacca aatagtggtc tctaaaacaa
    13981 ttccaatggg aagactctac tggcatgttc gtgccaagaa ggatgcctat aaatcagact
    14041 ggtcgcccac cttcagcttt gaatttgcca agtaagaact tatctttaca aagccataaa
    14101 ggcgtcttta aataaagtat gaataaaata attcttttta cttttttcct agcaataatt
    14161 ggctgcgcct ctgaagcgat aaaaacgccc tccgcctctc tctttggttc ttgggaggaa
    14221 ccaggtccca gttcttccaa cgaagtcatt ttagtgccgc agtggcatta ctcccctcaa
    14281 gttaacactc gaggtcttca agtgagttta cctcaagata aaaatcaaaa agcgattttt
    14341 gaagagatgt cctcttggat tcggtctcat tatatcaaaa acctcattgt cgagggttgt
    14401 gagggccagc tcgccgccgc ctctgatcta cgatttaatg attggtccct caatgatctc
    14461 aacggacaaa aaaatataga aaatgctcag actcatattg gccttaaaat tttaagtctc
    14521 tcaaagggag cgataaacgt cgaatgtggt gacaacttag ctcttatcaa agagcatcaa
    14581 ctggctttat cggatattcg tggtctggcc ggttacaaat taagacttga acaagcaaat
    14641 cttcctctaa aaaacagggc gtcctttgta gccggagcaa aagaagctct caaacttcct
    14701 aaggatacaa aagaagacct tgttcttaaa aagctaaacg aagaattgga tacagtgatt
    14761 agtcattttg agagtttagt cagtaaaaga aacgatgagt ttgtgaaaaa agctcgatct
    14821 cttgctggac gaaaagtcat cctcatcggc gctcttcaca ttgaagatct aaaatcaaaa
    14881 cttaatgaac aaaaaattcc gtttgcgatt tggcgcccta tcgggcttga aaatggtgaa
    14941 gtccacctta ttgaacaatt gagaagacag cttaagctgc cttctcatca ttagttccaa
    15001 atgaactcca atatcaattt ttagagcggg cgaaagaaaa ttaacgaact aatttttctt
    15061 tccgaaatgt ttttccatga ttttaccggt agcaacgacg atttctttgg aggcatgaac
    15121 actaatcttg agagcatcca ctcgttctac gaccttagat ccataccact tctcgatatt
    15181 atgataggcc atctcacgat cttcgatgtt ttcaaactca tagttcagtg tatccattaa
    15241 gtcctcctca ataaaaattt ttatttcata gactcaatat caatcctacg acgctatgac
    15301 tagtattaat agtgatgtgt tttttttaat acaattttac cttaactaga gtattcaccc
    15361 tagccttagg ccactttatt gaacttagac tggggaaaag gaagtttatt atcgacaatt
    15421 tcttccataa aagaaggaat atagcctgtt gactgcagtt tgccttcaag agttccatct
    15481 ggccttctgg tcatacggga catttgaaaa ataggtctga catcatagtt accctcgggc
    15541 agaattccca tgacttcgct gatggcggca atccgacggg acccatcaga aaatcgggaa
    15601 acttgaataa taatctcaat agcagacacc acctgagagc gcagagcctt ttcgctgatt
    15661 tgagcgtctc ccccctgagc cagcgcttcc acgcggacca tggcatcttc cggggagttt
    15721 gcatggacgg ttcccataca acccttgtga cccgtgttca tcgcatttaa taattccaga
    15781 gcttcagagg agcgaacctc ccctacaatt atacggtcag ggcgtaagcg caaagagctt
    15841 ttcaagagat ccttgatcgt cacttcgccc ttgcccagtt cattgccctg acgggtttcg
    15901 aacatcacca catgttcata atccacttgc agttcactcg aatcctcgat cacgataatg
    15961 cgttgtcctt tggggatacg cgtacagaga agacttaaaa gagtggtttt accagaacca
    16021 gttcccccgc tcaccagaat gttcttaccc agaaacatac agatatccag gaacctggcc
    16081 ccatcttcag tgatcgcccc gaatttaata taatctgaga atgagatttt gttattggtg
    16141 aatttacgaa tactgacgat agtccctttg cgggaacagg gcggaatcac cgctgcgata
    16201 cgagagccat caggcaaacg cgcgtccaac ctcggagttt catcattgat tcgacggccg
    16261 acactttgag cgatactatt caccgccgca cgaagggcat cttcatcact gaagctcgcg
    16321 ggaactctct caagctttcc cttacgctct acaaaaactt ctttgtgacc attgatcaga
    16381 atttctgaaa ccgtcttgtc ttccaaatat ggcaaaaccg gacctagatt attccggatg
    16441 gcatctttaa atacactcga cgtctctgac ataaaacacc ctgttaattc gaagccgaaa
    16501 tgacgatgat tcgtccttgc actgcggttt caggacattg ataagagaac accccctcca
    16561 cttttggatt caggttaaac acgcgctctt gcccatagag tgtgttatga tgttccgaaa
    16621 acgcatccaa tacgaaactg acatttttat ctttgctgtt cacattcacc acatgaatgc
    16681 ggtaactatt gccttttttt acccgaaggg tgtccggcat aaagcctttt tcagtattaa
    16741 gaatcacgat atcttgagtc ggctcgatac tatcaaagac ctgaccgaaa aggttttggg
    16801 tctcatccgg ttttgtgctg tcaggaagac ggttttgatt ggtcacgcga ttaaaatcca
    16861 attggcgacg tgacatatcc acctcccacc catggacaaa actgcttgga agcaaaagaa
    16921 atagcaaatt agtgcaaaca gctacgatcc tggaattcaa cgaattcacg tgcgacctcc
    16981 atagcgatat aattcacgac ttttacatcc atcatatgaa gcctttgaac cataaggtcc
    17041 ttatctccat cacaagcgtt gataacgtcc atggcgtgca ctatcatctc aggattttgc
    17101 gccatttcgt tgagcacttg ttcccagtcc aatttaagaa cagggtcgat cgcccctatt
    17161 gtgtagagcg catcaaatac cgtttgcaga tcaccttgta aaaacatacc aagcctctct
    17221 ttccaaaatc catcttggaa tctaaaggac ttatcggcga gagcgcgcag aacttgagtc
    17281 aaattgagac gtaggggaat aaaaatatta aaaatagaaa aggccggaga aattccggcc
    17341 ttttggcttg gtgcagtcac tctcttatta cagaatcact tcttttcttt tttcttctta
    17401 tcttcttcaa cctttttatt atcagcctct gcttttgcga tatttttcat cacgagagcg
    17461 gcgatttctg aaggcgattt atgagaactc atggtcctga gttttctttc attcactgct
    17521 tccttagaca attcaatatc tgcatccgca atcagcttaa gggttttacg ttcatggccc
    17581 tcccgctcgg cgaaaggctt gaaatccgaa atcacatttt ctaaaaatat cgcataggtg
    17641 ttctggacca caggcttgaa cgccttggga ttctttagag cgccgacggc ttcttgcacc
    17701 aaagcatcca tagtcgtctc ccattgctca ttttcatcca actcatggcg gagaggagga
    17761 attacttttt ccaccattcc atcatcattg gggcgagaat aaatggcctg aaccgcttct
    17821 tttaagattt cgatagatcc atctttttta aactccttga tctttttctg tgctaacgca
    17881 ttcatttgat cgagatcttt cagcgtcagc tgtgaataat tatagagaag ccccgcagaa
    17941 gagaccgaag cccacaataa acttgctaaa ataaataagt attttttcat gctctcagtg
    18001 taagcaaaga ttcattgaga ggaaaacaac tctgctggga cctagacctt tgtcctttgc
    18061 cacatctaac gcagtatctc caaaactccg tttttgattc agagcccctt gagtttgtgt
    18121 cagaatcgaa gtgattttaa caaaacacca acaattacgt atttcattta taccggagga
    18181 agatttgaac aaatcagcat tgatcgcact ttctattatg gcctttgcac tgaccaattg
    18241 tgcaccatct gcaaaacaaa ttaaagaagc tgttgaaaaa gatccaagca tcgttttcgt
    18301 ggccatcgaa aaatatccgg accaattcat cgaagttgtt aataaagcgg ctcaagacgc
    18361 tcaaaagaaa agccaagaca aagcgatggc tgaagagtct aaaaaacgcg atgaggagtt
    18421 tgccaatcct ttaaaaccag aagtccagcc tgaccgcgtg atctttggta ataaagacgc
    18481 aaaagtcact atcattgagt attcggattt tgagtgccct tattgcagca aaggattcca
    18541 aactgttaag caagttctta aagaataccc aaatgatgtt cgcgttgtat tcaaacattt
    18601 gcctttggat ttccatccac aagctatgcc tgcggcgaaa tactttgagg ccgtcgcaaa
    18661 gcaaagccca gaaaaagctg agaagttcca tgatgcggtt tttgagaatc aaggcgaact
    18721 tcgctctaaa ggcgaagatt ttttgaaaaa taccgctaaa aaattgggcg tcgacatgaa
    18781 gaaattggac aaagacgtta aagatgaaag catcatgaca cgtattcatg ccgacatgga
    18841 ggaagctaag aaattcaact tctctggcac tccaggcttc ttgatcaacg gtgtttctct
    18901 gcgtggagct tatccattcc cagagttcaa aacagtgatt gatcgtcact taggcaaatc
    18961 tgtagaaact aaaaaacaat aatctgatta ttttgcagtg attaaaaaaa gcccgacttt
    19021 agcactcgct aaccgggctt ttttaatttt cccaatacga ttcatgctcc ttgcttcaag
    19081 aaaaaacttt tagaaattga gcggtagaag tcgatcgctc aataccactt cataaggatt
    19141 caggtccaca acaaagggag aaattcgaag ccccttctta tgggcctcac gcaaaaggcg
    19201 tccgtactct ggatcgatct catccgccga tgaaaacttg tcacaatcat tgcgttgaat
    19261 agtaaacaat atttcagcgc tatggccttc cctcattaaa cttataagtt cttgtagatg
    19321 cttttgtccc cgtgtggttt cagcatcagg aaacattgcc gttttttctt cggccaaagt
    19381 gacattcttc acttcgatga aatgcttttt agcagaggcg tttctcatca aagcaaaatc
    19441 cagacgagtt tcggccgaga tcttaaattc gggtttcact acatcaaaag atgtccagtg
    19501 ctgccaggtc tttggccatt tttcttgcgc atttcccacc actctctcca gagtttcacg
    19561 aaccaccata ttgggcgtag cggtattcac tccgacccac acccctgtag gggcttgtat
    19621 catctcgagg gtctgcttga gtttgcgctc tggattatcg ctatgggaaa atagacaagc
    19681 ttggccagga tgattcacgc ttttcatact tcctgtattg ggaacatgag ccgttaaaat
    19741 ttgccctttc cattcaatat cggcaaaaaa tctcttatag cgttttaaga atacaccttc
    19801 atctaattgc atacgatatt tcatggaacc ccttcaaaag cctcaaattg acataacaaa
    19861 tttgtcagag caagctcaaa gccatgagac gatttgaaca ccaatggcag gaatttaaat
    19921 ggatcgacat cgaaggccct tctcaggagg attttgttca cttagccaat gagttcgaac
    19981 ttcccttacg ccccttagct aattgcctcg atcccgaaca tcttccacgc tgtgaattct
    20041 ttgactccat ggcttttttc attcttcgac attttgatac cacggccaaa gagtccgccg
    20101 gaacgatgca agatctcact actaagctgg ttttcgtggt cggtaaagat tatgtcctta
    20161 ccattcaccg cgcctccatc cactccatta acgatgagat aaggaaatgt gattttgaaa
    20221 atctttcctt ggtcaacttc atgcaaagcc tttgctctca ggtcatagag agctttgata
    20281 gtcccatcga aaatctcgaa gctaaaacag aaatcattga agaacgcgtc tttgcacttc
    20341 gccgccgcaa tattctgcgc gaaggctata ggatcaaaag aaagacttcg gcctatcgta
    20401 agctctttaa gttcactctc gatgtcctgg taaagctgca aactcgtcca gattttccat
    20461 taaatgatat cagcgatatt cgtgagtcct tggaccgctt gattttttat gtggataaca
    20521 tctacgagga catcactggt ctcttgaatc ttcacctctc gctgatgtcg caaaaaacca
    20581 atgaggcttc cttcaaaacc agcgaaatta tgcgtattct gactgttgtc tcgatcttct
    20641 ttctgccttt aaactttatt gcgggtgtct atggaatgaa tttcgaaaat atgcctgaac
    20701 tcaagaatgc caatggctat cactttgcac tcggtgcgat gcttttaatc gctttagcga
    20761 tccttttgtg gatctataaa aagggctggt taaaaaaaga ggatctctaa atcctctttt
    20821 ataactcctt acttagggac tactggggat tctcagcacc ttccaccaag gatctgtgaa
    20881 ttgaagcaga gctatggacg ccggatccag gacccaacga tccgagacct tattaacctt
    20941 aaaactaatg gaaagttgat agttattatt gcgaattccg atattgtttt tatatttaca
    21001 attgttggaa acattgccgg taatccgcac ttctatataa gtgcctttgt ccttgatttt
    21061 ttcccagtga acattatgaa acaaagatcc agcagggatc ctagtcatta actccaaatc
    21121 cggaacagga aagataggaa tgggcaaaga taaaacttcg cagtggacct ctttggcata
    21181 gatacggctc acatccatga catcttcacc cacattcaag agaacttcag gaaatgaatt
    21241 gagtaaatcg gaattcaaaa cacttgcgga aatctctgaa ggcgcccaag gaactaaagg
    21301 ggttgagatg attttttgtt gaagcaataa agaaactctt acaccaatca gatccaactc
    21361 ttcatggcta tgctctccat gatgatgacc taattcatga attaagatag ccacggcctc
    21421 agggatggtc acgggatcat aactgccggt ttcattcttg ctgtaaagaa gatccgcatt
    21481 aacctgaata gggcttccta ctttgtcccc tgtctttgcc acacgaacat tgccctcaat
    21541 aatgaaaaat cctggagttt tccgttcgga accaaagaaa atctgctgtt cctgcttctc
    21601 ttgcggaagc gcctgataaa ttttttccaa gagggcccgc tgcgtagcat tcaacttgca
    21661 ggactcagcc ttgagacaaa gttgaatgta agtgtccatt ttctcatagg cataaagaat
    21721 atttttttcc gcaagtccgc cgccgttatt gacaaaatcg ccgccgccgg cccagcaact
    21781 tacacttgag atgcttaaaa ggcttaaact taaaagaagt gctaacttaa tcatcgttat
    21841 tctccattaa ggtctgaagt ttggtcagag ggaaaagttg aaaagagagg gtgtaaactt
    21901 cagacgtctc tcctttgtct aacatgtcca caattttttt tcgagtttta agcaaaagct
    21961 ctttcgcaag aggtaatttt ttgagattca cagccagggt taccgagcca aactctcgat
    22021 attccacagg atcacggtgc aatgactgct ctgctaagag aagattttgg ctatgatgtt
    22081 tgcgaatcgc ggcagaagga atatcacgtg tggtggttaa agattttttt gtacgtacat
    22141 aacgaccccg tactttgcga aacagttcta aacgcatgag tcgttctaaa gactctttgg
    22201 cttcagaggc tttaattcct agtctttgag caatccattc aggattccca ttaaaacctt
    22261 ttaccttaga caaattcaaa acggcgaggt agtgccaatc tgctatcagt ctgaaaagat
    22321 cttcatctat aagcatctta tcatccactt catgcggacg attcaccttg tcacgatatt
    22381 catcccagag cttttctctt tctaatggag ataccagtaa cttttccaag actttttcaa
    22441 gattagtttt ggaaatattt ctcttccccc ccaacacctc tgacaaagta gcgacaccaa
    22501 tctctagatc acgggcgaag gcacgaatag aataagaagg atttcttgcc tgtctcgttg
    22561 tgagttcctg cattaataat tttttaatcg tcagttccat gggaacagat atatcttgag
    22621 ctatgccaaa agccaaggat cggaacaaac tgttccgatc tatcatgtcg gaacacaaaa
    22681 tctgatttag cactagacaa caactgcaat ttgaaatagt tctctacaac actaaaggca
    22741 acaacagttg cgaatagaaa caggacagaa cagaaacaac aggagaagaa aaaatgaaaa
    22801 aaattatctt ggcaatcgcg atggttttag gtgtgtctca agcacacgct cactcagttc
    22861 cagcatgggt ttgttctttg agcttcaaag gcgaagctga aggtttcaaa cttatctacg
    22921 gtgactatgc ctttaatggc aaaggagatc ttctttgcgc aaacccagcg ggcaaaagtg
    22981 tcgcttatcc agttattgta actatgaatt ccaaaaaatt atctccacag gtttcttttg
    23041 gtcgtatgag attgtctggt caggctgttg agttttctct cttcaaccat aatccggaag
    23101 atgttcttgg cacttattac atagcgcaag gtcaagctgc tgttgttggc ggaatcggtg
    23161 ctatcactgc aaccaaagtg cacttgccgg aaatcgctct tcacatttct ttgcaattcg
    23221 caagaggcct gggtgtgaac ttgggactta acaaaatggt gatcgctttg gattctaacg
    23281 aagcgccagc tccaactcct gttccagaag agccaattgc gcctgcgcct gcgcctgctc
    23341 ctgctccaac tccctactag tcagcatact tatacttagc gtttataaaa tcggagtgag
    23401 atttcaaaca tctcctccga tttttttctc tctccctctc gtgggagaga ccctctccat
    23461 ctattgactc atggcataaa gcgatgcaat tgcgtcttcc tgagcataag ttcaacacac
    23521 aagctcaaag gctcgtaact ctatgattta ttaatcttga agggagcaat ctccgcatgg
    23581 caacagacat aaaacttcct gaactcggtg aaggtgtttc tgaaggtgaa ttggtaaaat
    23641 ggttggtaaa accaggcgac agcattaaaa cagatcaacc cattgctgaa gtcatgaccg
    23701 acaaagccac tgttgaagtt cctactccca ctgcgggcat tgtcaaagaa ttgaaattca
    23761 aacctggcca agtggttaaa gtggatgcag ttatgatcac tcttgatgcg gccggagtaa
    23821 gtgccagcac tccaccaaaa tcaaacccaa ctgccgcggc gactcctacc acttctacga
    23881 cctctgcctc tacacctgtg aaattaccag aattgggtga aggtgtttca gaaggggagt
    23941 tggtaaaatg gctggtaaaa cccggggacg ctgtgaaagc ggatcaaccg attgccgaag
    24001 tgatgacaga caaagccact gttgaagttc ctactcctct tgctggaact gtcaaagaat
    24061 tgaaattcaa aaccggtgat gtagttaaag tggatagcat cttgttaatt cttgaaggcg
    24121 gcgcggctcc gaaaatctct cctcaaatat ctacaccaac tcccgcttct gtcagttatg
    24181 cagcgaccag cgcgagcatt catccccctg tagcggattc caaagtatta gcgactccag
    24241 ccactcgtcg acttgcacgg gaaatgaatg tcgatatcaa cgctttgaac ggcacaggcc
    24301 tagcaggtcg agtgactcgc gaagatgttt tctccatggg tgcgggcgcc actcgggccg
    24361 gagccgcagt tccagctcaa gctcccggtt taaatattat tcctcgcccg gcttaccaag
    24421 gcgggaccgg agcggaagaa cgcgtggcct tgatcgggat tcgtcgcaaa attgccgaaa
    24481 acatgcagaa atcaaaagcc atcattccgc atttcaccat tatggatcag gccaatgtta
    24541 ccgagcttgt ggctctgcgc gaatctctca aagattttgc agaaaaaaat ggaacaaaaa
    24601 ttacctattt acctatcgta attaaagcca tgatagcaac aatgcgtgaa ttccctatgt
    24661 tcaatgcttc cattgacgat gcggcttccg aaatcgttta caaaaaatat ttcaacattg
    24721 gattcgctgc ggatacacca aacggcttgg ttgtgccagt catcaagaat gccgagcaaa
    24781 aaaccatcat tgaactttct aaggaaatct tggacctttc aaaacgggcc cgtgatggaa
    24841 aactgaaagt ggaagaaatg aaaggggcat gcatcaccgt cactaacatc ggttctatag
    24901 gcggaactta tgccacgcca gtcattaacc atcctgaagt ggccatcgtg ggcatgtaca
    24961 aaattgatga aaaaccggtt atcaaaaatg gtcaattggc agctatcaaa gtgatgaact
    25021 atacagtcac ggcagatcat cgtctgatcg acggcgccgt cgctgcgaag ttcttaaaat
    25081 ccttccttga acgtattgaa aaccctgccc gtcttatgat ggatatgatt taggagttgc
    25141 catgcaaaat tttgacgtcg ttgttattgg ctctggccct ggtggttatg tggcggctat
    25201 tcgctcagcg caattgggtt ttaaaaccgc agtgattgaa agagaattct taggtggcgt
    25261 ctgcttgaac gtaggatgta ttccctctaa agccatgatt accgcaaccc atttgcttca
    25321 taaagcccag cacaacttcc caacaatggg attgaaaatc aaaggcgcta tcgaagtgga
    25381 tatgaaacaa cttgtagctt ggaagcaatc agtttgcgac aaaatgtccg gcggcgtaaa
    25441 tcagcttctc aagggaaaca gcgtcgttat tctgaaaggc gaagcggagt tcaagaattc
    25501 taaagaactc tctcttaaat cttcttctgg tgttgactcc atccaggcaa agtactttgt
    25561 cgtggctacc ggatcccgtc ccattgaaat ccctgctttt aagtttgacg aaaaagatgt
    25621 ggtctcttca accggcgctt tggcttggaa tgaaattcct aaacgcgttg taaccatcgg
    25681 cggcggctac attggtcttg agatctcttc ttacctgcga aaattggggt gcgaagtcac
    25741 cgttgtcgaa gcccaaagct ctttgctttc tggggttgtg gatcctgagt gtgcacaaat
    25801 agtgactcgc aagcttgaaa aatctggcgt gaaagttttg tacggagcaa aagccaaggg
    25861 acaaaagaaa aacaaagatg gctacgaagt caccgtggaa atcaatggca aagacgaggt
    25921 catcaaggca gataaaatat tggtaactgt tggacggcgt ccgaacggag accaagcgaa
    25981 cctcaaggca gccggtattg tcgtggatga aagaggtttt attaaagtcg atgcccaacg
    26041 tcgcacaagt gttcctcata tttttgctat tggggatata tgcggccaac caatgcttgc
    26101 gcataaagct tcccacgaag gagttttagt tgcggaagtg atcaaagggt acaaccgtgt
    26161 ttacgatgcg aaaacagttc ctgcggtggt gtttactgat cctgaaatcg cagcagcggg
    26221 gttgactgag gcggaagcaa aagcaaaggg ctttaacaat ttaaaagtcg caaaatttcc
    26281 gttcgcggca aatggtcgcg ctgtcagcat gatggaaaca gaaggcttcg taaaaatgat
    26341 cgcggatgct aagaccaatg ttttattggg tgttcacatc gtggggcccg aagcctcaaa
    26401 tttgatttca gaggctgttc tcgctattga aatgggagca cgaattgaag atcttgctct
    26461 atctatccat cctcacccta ctttaggaga ggccatgatg gaatgcgcgg aagccaccct
    26521 gggacacgcg atccacatta ttcagaagcc gctcactggc aacggcaaaa acgctcatgc
    26581 ttagctccaa caaaattcca ataaaaaaag ccgaggacac ctcctttcta ttacccacat
    26641 ctttcaaaga ggatgtgggt aatagaaagg aggttcgatg atgtaccata gattgttgat
    26701 tcgtactaag ttgtaaacgg caaggccgaa ggaaaacatc caaccaactt tcgcaagcct
    26761 cttaccattg gtcttctcat taaaccgatg gactttaacc agccaaaaat ttcctcaatc
    26821 ctttttcgct tgcgcagact tacttcgtag ccttcatgcc ttgttgttcg accatcaact
    26881 gagctcgtgt gtcgacgtga atggatattt tgtgcagtat gggacgcaat attcatttcc
    26941 cgcagcccct ctgtcagtga gtgttgatca taatgtttgt cggcaccaat tgtctttttc
    27001 ccaccgtcag caattccttg cgccatctcc agagcggcat ctggctccgc gtgataccca
    27061 ggttttgaaa tcctattatc aattgctaag ccattgcgat tttccattaa aacgtgcccc
    27121 atataggaca tcttggcagt ggcgcctttt gacttggtat atagctttgc ttcggggtcc
    27181 gtctttgact catgagtttc attgctgcgc ttttcgcctt gaaaattaac actggggttt
    27241 ccgccgccac tgcctggctt atcaccattc tctttattat cgtccttctt ctgaaaactt
    27301 tttagacttg cccacgcttc aatcaatgtt ccatctacag aaaaatgctc attcgatacg
    27361 agaccttttc tgctagcaag agcaacgact tctgtgaaaa acctatctgc aatttcacct
    27421 tcaattaaac gcttacggtt tgtggaaaaa ctggaacggt cccaaacctt gccggacatt
    27481 ccaagaccaa caaaccatcg gtacaacaaa ttataccgca actgttccac aagttgtgtt
    27541 tcactacgaa ttgtgaataa aacttgcacc agtaacgctt ttagaagctg ctctggagga
    27601 atgcttggtc gaccagtacc agcatagagc ccatcaaaat ctggccacat ttcttcaagt
    27661 attttatcaa cgaaatcttt gatttttcgt agaggatgcc ctttcggaat tgcatcttcc
    27721 attgaaacgt agctaaacat atcttcttgt tggtcttgtt tatatccgcg catgcgacat
    27781 tattacgaaa taattctaaa tatgaaaaga aaaatcctgg aggtggaaaa acaggcccga
    27841 agggccgttt ttcaccgacc tgtta
//
LOCUS       DATBTE010000466         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_339071, whole genome shotgun sequence.
ACCESSION   DATBTE010000466 DATBTE010000000
VERSION     DATBTE010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339071"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VIG33_17815"
     CDS             <1..482
                     /locus_tag="VIG33_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272755.1"
                     /translation="ITAPPGYELVSPPAGTVPPHYFWSITEAGFYPAQLLDKNHVSGS
                     YGGHPMANSVFFNMAQGQASHQGALTDSGSAGCIRMSMKSSAYVFNLVASTIAPKEQI
                     PKIDQYTGEFVPAADGSGEIEMNSGMKERSGRTTRLFGYKALYIINNAPDEDYSKLY"
     gene            complement(496..990)
                     /locus_tag="VIG33_17820"
     CDS             complement(496..990)
                     /locus_tag="VIG33_17820"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEY8272756.1"
                     /translation="MKFSDTLPQPIFKIVLIEPEIPQNTGNIGRTCVAANCELHVVGK
                     LGFEINDKNLKRAGLDYWPYLSFHRHESFEDWLKHVEDTSRIWLFTTKTKRTYFEPKY
                     VSGDWLVFGKETKGLAPDFLAKFPNQNVTIPMIGEGARSLNLATSVAIAAYEGVRQMI
                     YIKS"
     gene            1172..1510
                     /locus_tag="VIG33_17825"
     CDS             1172..1510
                     /locus_tag="VIG33_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY8272757.1"
                     /translation="MKKIEAIIKPFKLDDVVDALAEVGVEGISVTEIRGFGRQKGRTE
                     VYKGAEYVVDFLPKLKIEIVVPSALCEAAVEAIAKTARTGKIGDGKIFVLDVESVLRI
                     RTGEKNEEAL"
     gene            1530..>2510
                     /gene="glnA"
                     /locus_tag="VIG33_17830"
     CDS             1530..>2510
                     /gene="glnA"
                     /locus_tag="VIG33_17830"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEY8272758.1"
                     /translation="MNAQQALQFGKDKGARMVDLKFCDLVGTWQHITIPFTELSEEIF
                     EQGLGFDGSSIRGWRGIEESDMIILPEPSTAKMDPFMQVPTLSLICDVCMPETLQPYD
                     RDPRQVIKKAVAFMQSTGIADTAFFGPEAEFFIFDDVRFDQTNNSGFYMIDSDEAAWN
                     SGRDEGGKNLGYKIRHKEGYFPALPTDSQQDIRTEMCLELERAGMIVERHHHEVATAG
                     QAEINFRYDSALEMGDKMMWFKYIVKNVARRHNKTATFMPKPLFNDNGSGMHIHMSLW
                     KDGKNLFAGNKYAGLSELALYYIGGVLKHAPALCGIINPTTNSYKRLVPGF"
BASE COUNT          741 a          546 c          539 g          684 t
ORIGIN      
        1 caattacagc acctccaggc tatgagctgg tgtcaccccc agctggcact gtgccgccac
       61 actatttctg gtccatcacg gaagctggtt tttatccagc ccaactttta gacaaaaacc
      121 atgtctcggg atcctatggc ggacacccga tggcaaactc agtatttttt aacatggctc
      181 aggggcaggc ttcgcatcaa ggtgcattaa ctgatagcgg ctcagccgga tgtatcagaa
      241 tgagtatgaa aagctctgcc tatgttttta atcttgttgc tagtacaata gctccaaaag
      301 aacaaatacc taaaattgat caatacactg gtgaatttgt tcctgctgcc gatggaagcg
      361 gagagattga gatgaattca ggaatgaaag aaagatctgg ccgaacaaca agacttttcg
      421 gttacaaagc actttacatt attaataatg cccctgatga ggattactca aaattatatt
      481 aatcttaaaa atatcttatg attttatgta aatcatctgg cgaacccctt cgtaggccgc
      541 aatagcgaca ctcgtggcga gattcaaact gcgcgcacct tctccaatca taggaatggt
      601 cacgttttga tttggaaatt ttgctaaaaa atccggagca aggcccttgg tctcttttcc
      661 aaaaaccaac cagtctcctg acacgtactt gggttcaaaa taggtacgtt tcgtttttgt
      721 tgtaaataac cagatacgtg aagtgtcctc gacgtgtttc aaccagtctt caaaagattc
      781 gtgacgatga aagctcaaat agggccaata atctaacccc gcgcgtttta aattcttgtc
      841 gttgatttca aaaccgagtt tcccgaccac atgaagctca cagttcgcag ctacacaggt
      901 tcggccgata ttcccagtat tctggggaat ctctggctct atgagcacaa ttttaaaaat
      961 cggttggggc aaagtatctg aaaacttcat gcctccatct gataacgcaa gtcttcaacc
     1021 tttgtctacc atcagttcta ggaactctca actgtgtacc agaaacaaca acagcagcat
     1081 caaagccttc cgaagatatt ttgtcttgcg ttaacaatcg cttctttgac taaaaaatta
     1141 aatttgctat ctttctacaa cttgagaata catgaaaaaa attgaagcca tcattaagcc
     1201 ttttaaactg gatgacgtag ttgatgctct cgcagaagtt ggagttgaag gcatcagtgt
     1261 cactgaaatc aggggctttg gacgacaaaa aggccgtact gaagtatata aaggcgcgga
     1321 atatgtcgtg gattttctgc ctaaattaaa aattgaaatc gtggtgccta gtgcactttg
     1381 cgaagccgct gtagaagcga tcgcgaagac agcgcgtaca gggaaaattg gagacggaaa
     1441 aatctttgta cttgatgttg aaagtgtatt gcgcattcga accggtgaaa aaaacgaaga
     1501 agccttgtaa cctcatataa aggagacata tgaacgcaca gcaagctctg caatttggta
     1561 aagataaggg cgcccgcatg gtcgacctca agttctgcga tttagtcgga acttggcaac
     1621 acatcactat tcctttcact gaactcagtg aagaaatttt tgaacaggga ttaggttttg
     1681 atggaagctc catccgcgga tggagaggca tcgaggagtc tgacatgatc atcttgccag
     1741 agccatcgac agcaaaaatg gatcctttta tgcaagtccc taccttgtca ttaatttgcg
     1801 atgtgtgcat gcctgaaact ttgcaacctt acgatcgtga tcctcgccaa gtcatcaaaa
     1861 aggctgtggc atttatgcaa tccacaggta tcgcggatac agccttcttc ggacctgaag
     1921 ctgaattctt catttttgac gatgttcgtt ttgatcagac caataactct ggcttctata
     1981 tgattgatag cgatgaagcc gcttggaact cgggccgcga tgagggcgga aaaaatctcg
     2041 gttataaaat ccgccataaa gaaggttatt tcccagctct gccaacagac tctcaacaag
     2101 atattcgtac tgaaatgtgc ttagagcttg aaagagccgg tatgatcgtt gaacgtcatc
     2161 atcatgaggt ggcaacggcc ggacaagcgg agattaactt ccgttacgat tccgccttgg
     2221 aaatgggcga caaaatgatg tggttcaaat acatcgttaa aaacgtagcc cgtcgtcaca
     2281 ataaaacagc tacctttatg ccaaagccac tttttaatga taatggctcc ggcatgcata
     2341 tccatatgtc tctctggaaa gatggaaaaa atctttttgc gggaaacaaa tacgctggtc
     2401 tttctgaatt agcattatat tacattggcg gcgtattgaa gcatgcgcct gcattgtgcg
     2461 gtattatcaa tccaaccacc aattcttaca aacgtctggt tccaggattt
//
LOCUS       DATBTE010000467         5651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2251533, whole genome shotgun sequence.
ACCESSION   DATBTE010000467 DATBTE010000000
VERSION     DATBTE010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2251533"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2215
                     /locus_tag="VIG33_17835"
     CDS             <1..2215
                     /locus_tag="VIG33_17835"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEY8272759.1"
                     /translation="WTLEQTISLLKTISDQQETRIAMVSAVIGNRNHFISWLAGGEAP
                     VLEVHSTWRGPRRINAVWNTFPKWKVVRDEPMKVTKRSVYNRRTYHDLFGQLTVRTSL
                     TGGTLASQTSMPIGQLAMKYRIDQPAKRDFDSDNSLSHNDALYPLIELLAPRGPILCI
                     EATKRDTVALAKSIADKHPLADSDAIRELAEFVEAKLSNAHPLSRVVKHGVAYHHGSL
                     PNDIRTEIEKAVSDGIIKVLVATTTMTEGVNLPVQAVIISKVGTYQDNQEFLKIIKGP
                     KMANAIGRAGRATQETEGCVVLFHRGSVDAETFKALDPDDQEKWITSNLATKEFLDEI
                     AAYEALKLNNADAIFECRTPIASGFFSFAWFYLYTISPDGEPDQARIDRFLQNTMAWL
                     QATPEAKAAISSISTDLKTKYLATPTALRKIFAKSGSKLGTTKTLKAVFDAIKNDLVS
                     DVADLDLIKFILSDTTLPVILTVAEAPTKAVFNMRSGHRQEILIPLVSILEDWISGLS
                     YPDLADKYLAEVRDPDFRFEQLGDYITGYFDNFLPWVVGTILGWANESEDIEEALPVH
                     LPGLIRGGVPNEVALATLHQGIFSRQLAANIATWKQHNCPEVSKFRIRSELQKNSISK
                     LTQELNPSPSELRNLIEFLAEDKTQLLSSLNELGVCIIELWSTYNTALGECVIHGSGL
                     RDNFTLHRDDIHVGTIPTKYHVEIRTLRKIGLPINAIITADLKLELSVVGFESAE"
     gene            complement(2475..2828)
                     /locus_tag="VIG33_17840"
     CDS             complement(2475..2828)
                     /locus_tag="VIG33_17840"
                     /inference="COORDINATES: protein motif:HMM:NF022731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3088 domain-containing protein"
                     /protein_id="HEY8272760.1"
                     /translation="MNFVKDTIFLIEPNFEDPKYPGRRFYCWHCALIEGLLSSFPQFT
                     KNLDVIRIAWPRPRDAIISLVGEQNQTLPLMILTNDCELKLQTDFFDGIYFISDKNKI
                     LAALSERHGFPEQHP"
     gene            complement(2913..3074)
                     /locus_tag="VIG33_17845"
     CDS             complement(2913..3074)
                     /locus_tag="VIG33_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272761.1"
                     /translation="MKQMKVKIIEGYPVKPSKANKNAIPAALAWIGTQPLFLNAGFEI
                     VGNKTGGKQ"
     gene            complement(3302..3736)
                     /locus_tag="VIG33_17850"
     CDS             complement(3302..3736)
                     /locus_tag="VIG33_17850"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY8272762.1"
                     /translation="MSDTKKIHGSCLCQKVSVTLENEPKTFSVCHCESCRKWSGGVSM
                     SINGGEHLKFSGEEFVGRYSSSDWAERGFCKQCGTHLFYRLKKTDHYFLFAGLFGDAI
                     SPKFDLQEFIDQKPDYYSFSNETKSFTKAEGHKLLSEYLNRS"
     gene            complement(3861..5468)
                     /locus_tag="VIG33_17855"
     CDS             complement(3861..5468)
                     /locus_tag="VIG33_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272763.1"
                     /translation="MLGFPIQCKKLLDGKIILSTEHLEEFWNYAQSLSQLSPLADTEV
                     GLISVEDSLCGAAAVFLCLFRDWLKNFPDREKWCFDQILNTVAKPPPFMNFEVEGDIR
                     DHKWYHFCAIGAVVAWSENPKEKRWKEAIAYLCTSLHYKTVEILFSQSFKRREMLGGE
                     WLRLQHLVFRWAEKRFFISLVRNYPQGYGETSETFKTKLAGWRSGEITGFASNAIDST
                     LPPLSSFTFVNDQITALGKKELTERAQKWPRFDLDLVAHAFQWMPDLNDAHNNNERKS
                     WIAFWQNSLDQTIQVLQNAEQDDDVRGVQSKWDQWVFRGVVRLLTNLEPNENPDNFWK
                     PILSLGPAYYYWIQDFFREFFFCNLGKAEIPKNFVQNWKNIFEFCLSSLQWVYDKKRH
                     SHDLQDLWNSMASLPSMISELWIDKPLGILEEFKPFFERWLNHNFHRTKCAISMLEFL
                     ARYPELTIAFFVLVLVDEKYLAYDDHTWKKSEFGDAVAAFLEQLFLQRFDEVKSNAPA
                     LAAFQRVLKRLADTHNTIALEILAKMS"
BASE COUNT         1646 a         1285 c         1138 g         1582 t
ORIGIN      
        1 gtggacgctt gagcaaacaa tttcacttct taaaacgatc tctgaccaac aagaaacaag
       61 aatagcaatg gtctctgcag taattggtaa tcgcaaccat tttattagct ggctcgctgg
      121 cggagaagcg cctgtactgg aagtacactc tacgtggcgg ggcccacgaa gaatcaatgc
      181 cgtctggaat acatttccga aatggaaagt cgttcgagac gagcccatga aagtaactaa
      241 gcgatccgta tataatcgac gcacatacca tgatctcttt ggacaactta ctgttcgcac
      301 gtcgttgaca gggggtacgt tagcctctca gacttctatg ccaatcggcc aacttgccat
      361 gaaatataga atagatcagc cagcaaaacg agacttcgac agcgacaact ctcttagcca
      421 caacgacgcc ctatatcccc ttatcgaact cctagctcca cgaggtccta ttctatgtat
      481 cgaagccaca aagagagata ccgtagccct cgctaagtct atcgctgata aacatccgct
      541 ggcggattcc gatgctatta gagaactagc agaatttgtt gaagcaaaac tcagcaacgc
      601 acatccactt agcagagtcg taaagcatgg agtcgcttat caccatggtt cgcttcccaa
      661 cgatatacgg acagagattg agaaagctgt atctgatgga ataatcaaag ttttggtcgc
      721 gactaccacc atgactgaag gtgtgaatct gccagtccaa gcggtaatta tatccaaggt
      781 tggtacctat caagacaacc aagaattttt gaagatcatc aaaggtccaa aaatggccaa
      841 cgccataggc cgcgctggac gagccaccca agaaacagaa ggatgcgtcg tactgtttca
      901 tcgaggtagt gtagatgctg aaactttcaa ggcacttgat ccggatgacc aagaaaagtg
      961 gattacttca aacttagcta ccaaagaatt tcttgatgaa attgctgcat acgaggctct
     1021 taaacttaac aatgctgacg cgatatttga atgcagaact ccaattgctt caggcttctt
     1081 ttcattcgca tggttttacc tctatacgat ttctccggat ggagaacctg accaggcacg
     1141 aatagacaga ttcctacaaa acaccatggc atggctccaa gctacgccag aggccaaggc
     1201 agcaatttcg tctatttcaa cagatctcaa aaccaaatat ctcgctaccc caacggcatt
     1261 gagaaagatt tttgctaagt caggttcgaa actgggaacg actaagacat tgaaggcagt
     1321 atttgatgcc ataaaaaatg atctcgtcag tgatgtagcc gatttagatt taatcaaatt
     1381 catactttcc gacaccacgc tgcccgtgat tcttacagta gctgaagcac cgacaaaggc
     1441 tgttttcaac atgcgtagcg ggcaccgaca ggaaatcttg attcctctcg ttagcatcct
     1501 ggaagattgg ataagtggtc taagttaccc agatcttgcc gacaaatatc ttgccgaagt
     1561 tagagatccc gattttcgtt tcgaacagct tggagactac ataaccgggt attttgataa
     1621 ttttctgcct tgggttgttg gaacgattct tggttgggca aatgagtctg aagatatcga
     1681 ggaggctcta ccagtccact tgccgggcct tatacgaggc ggtgttccta acgaagtagc
     1741 actagcgaca ttgcatcaag gcatcttttc tcgccagctt gccgcgaaca ttgcgacatg
     1801 gaagcagcat aactgtcctg aggtttccaa atttcgaatt cgatcggagt tacaaaagaa
     1861 ttccatttct aagctaactc aggagttgaa ccctagtcca agcgagctca gaaatcttat
     1921 tgagtttctc gccgaggata agacacagtt gctcagcagc ctgaatgagc ttggtgtttg
     1981 tatcattgag ctttggagta cctacaatac ggctctgggt gaatgcgtaa tccatggatc
     2041 tgggcttcgg gacaacttca cgcttcatag agacgatatc cacgtaggaa cgatccccac
     2101 taagtatcac gtagaaatta gaacgctgag gaagattggc cttccgatta acgctataat
     2161 tacagcagac cttaagctgg aactctcggt cgttgggttt gaatctgcag agtaaattct
     2221 caaattccgc tggaaaccaa tgggaggatc aaatgtacct atttgacaat agatagctgg
     2281 cgttcaaaag aagatttcga gtcctttaag aacaaattcg ccgatgaata catggaaata
     2341 gaaaatctgc atctgatgca aggacgccca tcaaagaaat agtattggtt ttcttcatat
     2401 ttttcacttt cggtatttta aatacaactt atttatatat gttacttggg tcaagaaaaa
     2461 ctggtacagt caaattaagg atgttgctct ggaaagccat gccgttcaga taatgcagcc
     2521 agaattttat ttttgtctga aataaaataa attccatcaa aaaaatctgt ttgaagtttt
     2581 aattcacaat cattcgtcaa tatcatcaac ggtagagttt gattctgctc acccactaac
     2641 gaaataatgg cgtccctcgg gcgtggccag gcaattcgaa ttacatccaa attttttgta
     2701 aactgtggaa atgaagacaa taaaccttca attaaagcac aatgccagca atagaaacgt
     2761 cgtcctggat actttggatc ttcaaaatta ggttcaatta aaaatatcgt atctttaaca
     2821 aagttcatcg aacaatctct tttctttttc aaggccaaat ttgcaattct cactcgtcta
     2881 tattttttat tgatttatac ttcttttcga actcactgtt ttcctccagt tttgttccca
     2941 acgatttcaa acccggcatt taagaaaagt ggttgagtgc ctatccatgc taatgctgcc
     3001 ggaattgcat tcttgttggc tttgctgggc ttcactggat aaccctctat gattttgact
     3061 ttcatttgct tcatggcctt ttcagcagac gaatggcacg atagagcgag cttcgtattg
     3121 tgtgtgctct tgacccataa cagccgaaaa tcctcggttg cctggttctt gcattttgaa
     3181 ccgatgaaat tcggcaacgg catctttacc tgtcgagctt aaattgggtc ctggtccctg
     3241 acaagttgta gcgctggccg aaattaaaaa tgtgagcgca actgcaatcg ctttattcat
     3301 gttagcttcg attcaaatat tcgcttaata gtttatgacc ttcggccttt gtgaaagact
     3361 ttgtttcatt tgaaaaagaa taatagtcag gcttttggtc aataaattct tgaagatcaa
     3421 acttagggga aatcgcgtcg ccaaagagcc ccgcaaatag aaagtagtga tcggtctttt
     3481 taaggcgata aaaaaggtgc gtaccgcatt gtttgcaaaa gccacgctcg gcccaatccg
     3541 aagaagaata tctcccgaca aactcttcgc cagagaattt taggtgttcg ccaccattga
     3601 tgctcatgga gacgccaccg ctccattttc gacaagattc gcaatgacat acagagaaag
     3661 tcttaggttc gttttcaaga gtcacactga ctttctggca gaggcagctt ccatggatct
     3721 tttttgtatc actcataagt ttaccttctt ttaaacttct taaggttcat cgaaatagct
     3781 tcagtaccaa tcggtctcgt agtgatcagc agctttagag tcaattactc tctttcttac
     3841 aacactcacc gaactatagc tcaagacatc tttgccagaa tttccaacgc tatagtgttg
     3901 tgtgtgtcag ccaatctttt caatactcgc tgaaatgctg ccaacgccgg agcattcgat
     3961 tttacctcat caaatctttg taaaaatagt tgttccaaaa acgcagccac tgcatcaccg
     4021 aactcgctct ttttccatgt gtggtcgtca tacgcgagat atttttcatc aaccaggacc
     4081 aaaacaaaaa aggcgattgt tagctctgga taacgagcta agaactccag catcgaaata
     4141 gcacatttag ttcgatgaaa attatgattt agccatctct cgaaaaaagg tttgaactct
     4201 tctagaattc ccagtggctt atcaatccaa agttcagaaa tcatactcgg cagcgaggcc
     4261 atgctattcc aaagatcctg gagatcatga gaatgacgct ttttatcata aacccattga
     4321 agagaactta agcaaaactc aaaaatgttt ttccagttct gcacaaagtt tttaggaatc
     4381 tctgcttttc ccaaattaca aaaaaagaat tcacgaaaga aatcttgaat ccagtagtaa
     4441 tatgctggtc caagagatag aataggtttc caaaaattgt ctggattctc atttggctcg
     4501 agattcgtta acaaacgaac aacgcccctg aaaacccatt ggtcccattt ggactgtacc
     4561 cctctaacgt cgtcgtcttg ttccgcattc tgaagaacct gtattgtctg atctaaacta
     4621 ttttgccaaa atgcgatcca actttttcgt tcgttattat tatgagcatc attaaggtct
     4681 ggcatccatt ggaatgcatg cgcgacaaga tccaagtcaa aacgtggcca tttttgagcc
     4741 cgctcggtca gttccttttt tcctagagca gtaatttgat cattgacgaa agtaaacgaa
     4801 gacaacggcg gtaaggtaga gtcaatcgca ttggatgcaa atcctgttat ttctccgctt
     4861 ctccatcctg caagttttgt cttgaatgtc tcgctcgttt cgccatatcc ctgtggataa
     4921 ttccgaacta gagaaataaa aaatctcttt tctgcccaac ggaaaactaa gtgctgcaaa
     4981 cgaagccact cgcccccgag catctcacgt cttttaaaac tctgagaaaa taaaatctct
     5041 acggtcttgt agtgcagact cgtacacaaa taagcgatag cttccttcca tcgtttttct
     5101 ttcggattct cggaccaagc gacaaccgca cctatagcac aaaagtgata ccacttatga
     5161 tcacgaatat ccccttcaac ttcgaaattc ataaatggcg gtggcttcgc caccgtattt
     5221 aagatctgat caaaacacca cttctcacgg tctggaaaat ttttgagcca atcgcggaac
     5281 agacaaagaa aaacagcggc ggctccacaa agtgaatcct caactgaaat tagccctacc
     5341 tctgtgtcag ccaaaggaga taactgcgaa agactctggg catagttcca aaattcttcg
     5401 aggtgctccg ttgaaagaat tatctttcca tccaataatt ttttgcattg aatcgggaag
     5461 cctagcaata tttgctgctc gttcgcttct tttacttgag cagcattatt gtggaagcgc
     5521 cggtttaaaa aggggccatt ttctccggtt taccggtccc atgtggtcca ccatagccgg
     5581 tttaagagtg gaccattcac catcgcatag tgggcggcct cggccttcga cgttttagcc
     5641 gagccgatgc c
//
LOCUS       DATBTE010000468         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2319932, whole genome shotgun sequence.
ACCESSION   DATBTE010000468 DATBTE010000000
VERSION     DATBTE010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2319932"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VIG33_17860"
     CDS             <1..527
                     /locus_tag="VIG33_17860"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8272764.1"
                     /translation="KFDIDPPKIYFRSGEFKAYKEKSEKNYFAATSEDAFSPTMTAEG
                     FRKVGVLQSLLEKNALGSGRNGPLFWDEPEANMNPKLMRMMVEVLLELSRSHQQVIVA
                     THDYVLLKWFALLKDDGKGDHVRFHILHREPMSKKLKLSSTDEYLEIKPNPISDAFRD
                     LTVTHAKQNTGMKK"
     gene            524..1051
                     /locus_tag="VIG33_17865"
     CDS             524..1051
                     /locus_tag="VIG33_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272765.1"
                     /translation="MNELEELDLAIVFNNAVDLPLKFDQQDPDHDNFHDLVNMPRVDF
                     VVEMDKEVYFIELKDTNRPDAADPGGATFMKKIASGKVEQSLAEKYLYTFFFRWAENQ
                     LNKQVHYVCLITLEDPMVNILADDLATTLRHLLKPSKRWTRQPLASCQVHSLQSWAEA
                     FPEWPIRRLSDEVAS"
     gene            1063..>2095
                     /locus_tag="VIG33_17870"
     CDS             1063..>2095
                     /locus_tag="VIG33_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272766.1"
                     /translation="MENTKLKKFAQFARRSLIEQVSAKLRHVLSEDSASRRENVEVVR
                     KLEEEIHIQGKDQVIERVAYIWFNRFCALRFMDVNRYTRIGVVSPAEGQFQPEILAEA
                     KMGHIDDEMVHDRIRQQIFALLGGNAPSRDPQGEAYHLLVVAACNYWNKAMPFLFERI
                     EDYTELLMPDDLLSGNSILAYTREAMTPDVCEDVEVIGWLYQFYISEKKDAVFEGLKK
                     NQKITPENIPAATQLFTPHWIVRYLVENSLGRLWLLNRPDSKLVEQMDYYIKPEQIET
                     DFLRIGKPEEIKICDPACGSGHMLTYAFDLLYAIYEKEGYEPTEIPEKILTHNLFGLE
                     IDERAGELAA"
BASE COUNT          610 a          487 c          482 g          516 t
ORIGIN      
        1 gtaaattcga tattgatccg ccaaaaattt atttcagatc aggtgaattc aaagcctata
       61 aagaaaaatc tgagaaaaat tattttgctg ccacttcgga ggatgcattt tccccaacga
      121 tgaccgccga agggtttcgc aaggtcggcg tgttacaaag tttacttgaa aaaaatgcat
      181 taggatcagg cagaaatggc cctcttttct gggatgagcc agaagccaat atgaatccca
      241 aattaatgcg catgatggtt gaagtattgt tagaactgtc tcgcagtcat cagcaagtta
      301 ttgttgcaac ccatgactat gttttgctca agtggttcgc tctgctaaag gatgatggta
      361 agggagatca cgttcgtttt catattctgc acagagaacc gatgtcaaaa aaactgaagc
      421 tctcttctac tgatgaatat ctagaaatca agccaaaccc aatttcagat gcgtttcgtg
      481 accttactgt aacgcatgct aagcaaaata ctggaatgaa gaaatgaatg agcttgaaga
      541 attggactta gccatcgtat ttaacaatgc agtggatctc ccactgaagt ttgaccaaca
      601 agaccctgac catgataatt ttcacgatct tgtgaatatg ccacgtgtag attttgtagt
      661 tgagatggat aaagaagttt atttcatcga gctaaaagat actaacagac ccgatgcagc
      721 cgatccgggt ggcgctactt tcatgaaaaa aatcgcaagc ggtaaagtcg aacaaagcct
      781 agccgagaaa tacctttata catttttctt ccgctgggca gaaaaccagc tcaataaaca
      841 agttcactat gtgtgcctga ttacacttga agacccaatg gttaatatct tagctgatga
      901 tctcgctacc acattgcgac atttactaaa accatcgaaa agatggacgc gacaaccact
      961 tgccagttgc caagttcata gtttgcagtc ttgggcagaa gcattcccag aatggccgat
     1021 acgcagatta tcagatgagg tagcatcgta aataaggaaa aaatggaaaa cacaaaacta
     1081 aaaaaatttg cgcagttcgc ccgacgcagc ctgatcgaac aagtttcagc caaactcaga
     1141 catgttcttt ctgaagatag cgcctctcgg cgtgaaaatg tggaagtcgt tagaaaactg
     1201 gaagaagaga tccatattca gggtaaagac caagtaattg aacgggtggc ctatatctgg
     1261 ttcaaccgct tctgcgcttt gcgctttatg gatgttaacc gttacactcg catcggcgtg
     1321 gtttctcccg ccgaaggaca gttccagccc gaaattctgg ccgaagccaa gatgggccat
     1381 atcgatgacg agatggtgca tgacagaatt cgacagcaaa tctttgctct tctcggcggc
     1441 aacgctccca gccgcgatcc gcaaggcgag gcctatcacc tgctagttgt cgccgcttgt
     1501 aactactgga acaaggccat gccgttcctg tttgagcgta tcgaagacta caccgaattg
     1561 ttgatgcccg acgatctgct ctcgggcaac tccatcctcg cttatacccg cgaagccatg
     1621 acgccggatg tctgcgagga tgtcgaagtg atcggctggc tttaccagtt ttacatttcc
     1681 gagaaaaagg atgcggtgtt tgaagggctg aagaagaatc agaagatcac gccagaaaac
     1741 atccccgcag ctacccagct tttcactccg cattggatcg tgcgctacct tgtggaaaat
     1801 tctctaggac gtttgtggct gctcaaccgt cctgactcga agctggtcga gcagatggac
     1861 tactacatca agcccgaaca gatcgagacg gactttctac gcataggtaa accggaagag
     1921 atcaaaatct gcgacccggc ctgcggctcc ggccacatgc tgacctatgc tttcgacctg
     1981 ctctatgcca tttatgagaa ggaaggctac gagcctaccg aaattccaga gaagatacta
     2041 acccacaatc tctttgggct agagatcgac gaacgcgcgg gcgagctggc tgctt
//
LOCUS       DATBTE010000469         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_749162, whole genome shotgun sequence.
ACCESSION   DATBTE010000469 DATBTE010000000
VERSION     DATBTE010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749162"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VIG33_17875"
     CDS             complement(<1..420)
                     /locus_tag="VIG33_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272767.1"
                     /translation="MFSKALVLPTLMLAVFSIFPATMGRLKFIPIYKTLMVKANKKLP
                     PHIAYLDSHFDIQNSEWSDFEKKSKRPLAVGLSENLIQQGSYVFTQKRELEGLVIHKN
                     SELLVLASEPVYKTNIQITQTPEAPQWLQNIVREPAVN"
     gene            complement(470..907)
                     /locus_tag="VIG33_17880"
     CDS             complement(470..907)
                     /locus_tag="VIG33_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272768.1"
                     /translation="MKIFGFSFLFSIFIFIGCQTQGVYLRETPLNISETRKAVVSVIG
                     QPRVTSENGRELLSKYYDRKGNSEDQIKMARERFSTHVIILGDRRPYDIQVEVVVEVR
                     DHAGVYQVVEKDETRARKIALDVQKALVQSRDNRNIIDDFKPY"
     gene            complement(909..1556)
                     /gene="fsa"
                     /locus_tag="VIG33_17885"
     CDS             complement(909..1556)
                     /gene="fsa"
                     /locus_tag="VIG33_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEY8272769.1"
                     /translation="MKFFIDTAEIEEIRQANLRGWVDGVTTNPSLIAKSGKDFFTVIK
                     EICKEVSGPVSAEVISLKSDEMVREGLELAKIASNVVVKIPMCEDGMIAVKKLKAEGV
                     RTNVTLVFSPMQALLAAKAGASMVSPFVGRLDDIGVDGMEMVAQVIQMYQNYDFNTEV
                     LVASVRSPMHIQMAAEMGADIATIPFKVMQQMTHHPLTDKGIKVFLDDWNKVQKK"
     gene            complement(1596..2081)
                     /locus_tag="VIG33_17890"
     CDS             complement(1596..2081)
                     /locus_tag="VIG33_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272770.1"
                     /translation="MNVFNHQTLVHLATDLVGGTPIARKLMAELRKLGHISAVSSIYK
                     RYLGTNRVDLNANIEAVLRFTTNCAVDELLWHFRRLEKDNRISLNLLTYDDLVVLSPQ
                     LTLPSPHLHADFLIVRCASEAWPQFEHPIMEKTLSEISALALPAVDAEFFLQGKSLVD
                     F"
     gene            complement(2081..2797)
                     /gene="dapB"
                     /locus_tag="VIG33_17895"
     CDS             complement(2081..2797)
                     /gene="dapB"
                     /locus_tag="VIG33_17895"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY8272771.1"
                     /translation="MKKKLKVGLVGASGRMGQEITAILKTKDELELHLAICRSASLGE
                     FAYTRPTPNCKEAKEVEIWIDFSSPELLIKLLKVTIKNKTPLVSGTTGLTASNYAALE
                     KAAKVIPVLWASNMSLGVAVITEALEVFSRIRQFDFQIEEFHHNRKKDRPSGTAKSLQ
                     EKLEQTVGRKLPEPLSIRGGGIFGVHKIHAMSEEEHIIFEHMALNRAVFARGAVEAAL
                     WLGSKRKGFYSMRDVLYSGS"
     gene            complement(2798..>2951)
                     /locus_tag="VIG33_17900"
     CDS             complement(2798..>2951)
                     /locus_tag="VIG33_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEY8272772.1"
                     /translation="LFVEANPIPVKMALYYMGIIESPELRLPLVSMSSDLADKMKREM
                     KALEVI"
BASE COUNT          862 a          676 c          537 g          876 t
ORIGIN      
        1 atttaccgcg ggttctcgaa caatattttg tagccattgt ggggcttcag gcgtctgagt
       61 aatttggata ttggttttat aaaccggttc tgaggccaaa actaaaagct cagaattttt
      121 atgaattacc agaccttcaa gttctctctt ttgggtgaac acatacgaac cctgctgtat
      181 taaattttca gaaagtccaa cggctagcgg acgcttggat tttttttcaa aatcactcca
      241 ctcggaattc tgaatatcga agtgactatc aagataagct atatggggag gaagtttttt
      301 attcgcttta accatgagtg ttttataaat aggaataaac ttcaatcttc ccatggtagc
      361 aggaaaaatt gaaaataccg caagcataag agtaggcaga actaatgcct tactaaacaa
      421 acattcacct caacttcaaa gtctttattt aattactctt ctatagtatt taatagggct
      481 tgaaatcgtc aatgatgttg cgattgtcgc gactttggac aagcgctttc tgcacgtcta
      541 gtgcgatttt gcgggctcgc gtttcatctt tttcaacaac ctgatagacc cctgcatgat
      601 cacgaacttc aaccacgacc tcaacctgga tgtcgtaagg tctacgatct cccagaatta
      661 taacatgagt tgagaatcgt tctcgcgcca tcttaatctg gtcctcacta ttccctttgc
      721 ggtcatagta tttagaaagc agttcacggc cattttcact cgtcaccctt ggttgcccga
      781 tgaccgagac aacggccttg cgtgtttcac tgatattcaa aggggtttcc cgtaaataga
      841 ctccctgggt ttgacaacca ataaaaataa agattgagaa taaaaacgag aacccaaata
      901 ttttcatatt attttttttg taccttattc caatcatcca aaaagacttt aatccctttg
      961 tcagtaagtg gatgatgggt catctgctgc atcaccttga acggaatagt tgcaatatct
     1021 gcacccattt ccgcagccat ttgaatatgc ataggactac gaacacttgc taccaagact
     1081 tccgtgttaa aatcataatt ttgatacatc tgaatcactt gagcaaccat ttccatgcca
     1141 tcgacaccaa tgtcatcaag acgtccgacg aagggagaaa ccatacttgc tcctgctttc
     1201 gcagcaagca aagcttgcat cggagaaaaa acaagagtta cattcgtgcg aactccttca
     1261 gctttcaatt tttttaccgc tatcattcca tcttcacaca ttggaatttt gacaaccaca
     1321 ttacttgcaa ttttcgcaag ctcaagtcct tcgcgaacca tttcatctga tttcaggcta
     1381 atcacttcag ctgaaacagg accagaaact tctttacaga tttctttgat cacagtgaaa
     1441 aaatcttttc ctgatttagc aatcaaagaa ggatttgttg tgacaccatc cacccaacct
     1501 cgcaagttag cttgtctgat ctcttcaatt tccgctgtat caataaaaaa cttcatgatt
     1561 tcctccgatt tcccactctt gtacgtccac tagcgctaaa aatcaaccaa gctttttcct
     1621 tgcaaaaaaa actcagcatc tactgcggga agcgccaagg ccgaaatttc actcagtgtc
     1681 ttttccataa tcggatgctc aaactggggc catgcctcag aggcacaacg aacgattaag
     1741 aagtcagcat gaagatgagg acttggcaat gtaagttgcg gcgataatac aaccagatca
     1801 tcataggtta aaaggttgag tgaaattcta ttatcttttt ccagtcgacg gaaatgccaa
     1861 agcaactcgt cgacagcgca attcgtagta aaacgaagga cggcttcaat atttgcattg
     1921 agatctactc gatttgttcc cagatatctt ttataaattg atgaaacagc ggagatatgg
     1981 ccaagttttc tcaactcggc cataagtttt cgagcaatag gggttccccc tacaagatcc
     2041 gttgctaagt gtaccaaggt ctggtgatta aatacattca tcaacttcca ctataaagaa
     2101 catcacgcat actatagaag ccttttctct tagaacccaa ccatagggcg gcttcgacag
     2161 ctcctcgagc aaaaaccgct cgatttaagg ccatatgttc aaatataata tgttcttctt
     2221 cagacatcgc atgaatttta tgtactccaa aaataccgcc cccgcgaatt gatagaggct
     2281 ccggcaattt acgtcctact gtctgttcca atttttcttg aagactcttc gcggttccac
     2341 tgggtctgtc ttttttccga ttatgatgaa actcttcaat ttgaaaatca aactgacgaa
     2401 ttcgcgaaaa tacttctaat gcttcagtga taacagccac tcccaagctc atatttgaag
     2461 cccataaaac aggaattacc tttgccgcct tttcaagggc agcataattg gatgctgtta
     2521 agccagtggt tccagaaacc aggggggttt tatttttaat agttactttg agaagtttaa
     2581 tcaaaagctc aggacttgaa aaatcgatcc aaatttcaac ttcctttgct tctttacagt
     2641 tgggtgttgg acgggtgtat gcaaactctc ctaaagaggc agaacgacaa atagcgagat
     2701 gaagttcaag ttcatcttta gtttttaaga tcgcggtgat ctcctgtccc atgcgaccac
     2761 tcgcgccaac caaaccaact tttaactttt ttttcattta gatcacttcc agcgctttca
     2821 tttctcgctt catcttatcc gcaagatcac tggacatact taccagaggc aggcgcagtt
     2881 caggagactc aataattccc atgtaataga gtgccatttt cactgggata ggattggcct
     2941 ccacaaagag a
//
LOCUS       DATBTE010000470         1954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1977501, whole genome shotgun sequence.
ACCESSION   DATBTE010000470 DATBTE010000000
VERSION     DATBTE010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1977501"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VIG33_17905"
     CDS             <1..442
                     /locus_tag="VIG33_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272773.1"
                     /translation="FIYKASSRPRNHWMYVYDEDSAFARLHFPKRLTEQSEDPIQAVQ
                     AEVYSSKLKKLDSHSTQPERIVGALEKMGILKSSEIISSDIRFCKYANVIFDHQRKSN
                     IDSILNFLNNNNIIPIGRFGKWAYLWSDESYLDGYNSLKNKFTL"
     gene            1094..>1954
                     /locus_tag="VIG33_17910"
     CDS             1094..>1954
                     /locus_tag="VIG33_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272774.1"
                     /translation="MKWGSLITERIDLFPTFFSGAMFLVCLIGFIDFFKGAPRRALLA
                     FILFLCFPIARWHLTTAYMDPTLMVGILATFMSAAAYLNHRKDKYFYLLAFFSAVSFS
                     VKHFGAFYALPIVLWAFIELIRQEKRLRWGLLIQALALALLPIIVFYHYNYVTTGSFF
                     FPFVFTENNQLFWDLDSVKSFNAAINAFSGGSGFLDLLKLPLTIGTSEKHSDTSQFAV
                     GLLWAPVWLIANCIFFFRRYKKKPFDPVEFLFFTTVNLQTIIWYKTSPVFRYLTCEMM
                     LMSLFALWITE"
BASE COUNT          488 a          454 c          338 g          674 t
ORIGIN      
        1 gttcatttac aaagcctcct cacggcctag aaaccattgg atgtatgtct atgatgaaga
       61 ttctgctttt gcgagactcc acttccctaa acgattgact gaacaatcgg aagatccaat
      121 acaggccgtc caagctgaag tgtattcttc aaagcttaaa aaattagatt cgcactccac
      181 tcaacctgaa cgaatcgtgg gtgcgcttga aaaaatgggt attctgaaat cttctgagat
      241 tatctcgtcg gacattcgct tctgcaaata cgccaatgtg atattcgatc atcaacgaaa
      301 atccaatatc gattctattt taaattttct gaataataat aacattattc ccattggaag
      361 atttggaaaa tgggcctatt tatggtcaga tgaaagctac ctggatggat ataacagtct
      421 aaaaaataaa tttactctgt aagttttaac tttacaaaaa atggacaact aaatgcttaa
      481 aacaaatttt aaatattata cgttcattag tttgtcctgg atcaccctct ttgcaataat
      541 cctcagagtc ggatcttcat ggaatataaa tcattggctc ttttttgccc tccagatcct
      601 cctaccgatc atgtatttat tcccctttgc ctccctagct tggcgttttt ctaaaaatct
      661 agagtaccgt ctttttttct cgataacttt atctttatcg gcgctcgcga tttttaactt
      721 catcacagca agattctttc tgcttaacct ttacctccag tggggcgcat ggtccctgtg
      781 ccttattccg gaagctttca ctggatttcc ttttttgaga agcagtttga ccccccttag
      841 atctttctta aaaatcaata gtctcacatc ctgtttcaat catattttat gttgcacttt
      901 agccttgctg tttgccctga cgtggctgct tcaccccagc cttggagcta cttatccacc
      961 cactactttt gacctttcct tactccacct cactcttcca cagacgtata tttacaatga
     1021 aggaattgta aattcatggt ggctaagagg ccctttcgta cctcagctgg tccatatttt
     1081 attcgccggg ggaatgaagt ggggctcctt aatcactgaa cgtattgatt tgtttccaac
     1141 ttttttttcc ggtgctatgt ttttagtctg ccttataggc ttcatcgact tttttaaggg
     1201 cgccccacgc cgagcacttt tagcttttat tttatttctt tgctttccca tcgcccgctg
     1261 gcacctaacg accgcctaca tggaccccac gcttatggtc ggcatcttgg caactttcat
     1321 gtcagcagca gcctatttaa accatagaaa agataaatat ttttatcttt tggccttctt
     1381 ttccgcagtc tctttttccg ttaagcactt tggcgccttt tacgccttac ccattgtctt
     1441 atgggccttc attgaattga tacggcaaga aaaaagactt cgttggggac tcctcattca
     1501 agcgctcgcc ttggctctgc ttccaataat agtattttac cactataatt atgtaacaac
     1561 agggtccttt tttttccctt ttgttttcac agagaacaac cagctttttt gggacttaga
     1621 tagcgttaag agttttaatg ctgcgatcaa tgcattcagt ggagggagcg ggtttttaga
     1681 tttgctaaaa cttcctttaa ccataggtac ctcagaaaaa catagtgaca cctctcaatt
     1741 cgctgtgggc cttctttggg ctccggtctg gcttatcgcc aattgcattt ttttctttcg
     1801 tcgctacaag aagaaaccct ttgatcccgt tgaattcttg ttttttacaa ctgtgaattt
     1861 gcaaactatc atttggtaca aaacaagtcc tgtctttaga tatctcacct gtgaaatgat
     1921 gctcatgtct ctgtttgcct tatggatcac ggaa
//
LOCUS       DATBTE010000471         5206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_885877, whole genome shotgun sequence.
ACCESSION   DATBTE010000471 DATBTE010000000
VERSION     DATBTE010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885877"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VIG33_17915"
     CDS             complement(<1..327)
                     /locus_tag="VIG33_17915"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY8272775.1"
                     /translation="MKFLTKEQVISHFKVLILGAGTGGVAVAARLNKKMNGKDIGLIE
                     PAEYHFYQPLWTLVGAGVVPKEKTRKPMSELMPNGVSWIRDRVQSVDAIAKKVACVSG
                     KEITYDY"
     gene            complement(497..1255)
                     /locus_tag="VIG33_17920"
     CDS             complement(497..1255)
                     /locus_tag="VIG33_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8272776.1"
                     /translation="MAELENKRIFLVVTPNAELNTQLEETIKAHVQNATVFTAVDGVE
                     ALFKCENVPPHVVVVDSQISKLSAVDLTYNILKRKDRAAVIILAPVADEDRFIDEVVT
                     GQVQFLSGSGQVKVFNNHITRALNWVSHEDKSIYHLRFLSPNEYLIKEGETADFVYIL
                     KSGELRAYKTENANEILLGTIYPGEFVGEMAYINGEARSADVISITDCELIEIPSVSL
                     DAILFSKPAWSKALLKTLSKRVKNSNEEKSNKTT"
     gene            1422..2204
                     /locus_tag="VIG33_17925"
     CDS             1422..2204
                     /locus_tag="VIG33_17925"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY8272777.1"
                     /translation="MTKSNKRIGVFAILAGWALLIMFFQNMTFIEFSTLNLPLVDEDS
                     RQDQARELLGNYYKGSLAQRFEGEQSLNYLVYKKIDASMPDRWKNRLPEITEALIAES
                     QKQELDPIFVLAVIQTESNFNTDARGTSGEIGLMQILPKTAEWISKKYKMPWKGSRSL
                     FDPIVNIKIGVTYFAHLRDEFESRAYHYLPAYNMGPRNMRKVSRTIGSISSTGRIIKR
                     DYAMRVMKNYTNIYEQMVVEQKELIKLAKNQNSSKISEKLSR"
     gene            2332..3729
                     /locus_tag="VIG33_17930"
     CDS             2332..3729
                     /locus_tag="VIG33_17930"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEY8272778.1"
                     /translation="MNKLKLSFLGFILSAIYLLTACTGNLAKDLETDRDHEKDSDFYL
                     AAVEDQNASPDLIRKKLSYFFLVEQFNVGINGSMLAAIKSNPPGGILFWNGNNADATL
                     LRESVRAYSQQAEASNLNPLLFSTDYEGGALHQSPNGTKTPGVQRFTKGFSSLAHPRW
                     LGISMKEYGTELCILHGKIISQELKSVGINYPLSVVSDLATQTLTFARAISKNPEETS
                     LCITKIFEQFIKTKDIIFVTKHFPGLGLTKGDTHEGTVTATTTDEEILSAHLKPFADL
                     IRFSKSNAAEKLLSIMTTHAKFLAYDGDHVTTEAPKIVTELLKKKMSFEGLVVSDAMW
                     MGEYGKMKSMQLMSVYLNSFLSGIDLLMIPGVRFAESVNYFRKVYDGSLTAEEKEALT
                     DRTGLSWQETQEKFMARIAQSLNAHDTARSKIKAPHIYIQKSETPSNLTSADRARYNE
                     ILSQLSIHGNSLQKD"
     gene            3742..4221
                     /locus_tag="VIG33_17935"
     CDS             3742..4221
                     /locus_tag="VIG33_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEY8272779.1"
                     /translation="MDKKKLIAHIRAELEKDLLILKEVARVTREAATHEESKPENQYD
                     TRALEASYLAGAQSKRITETEDVLVVYKHIEPKYFTEQDSISATALVEAELNGKKSYF
                     FVMSKGGGLTVTFEGTKIQVITPGSPLGGALLGLKVGDLALLDKGSQILEYEILSIQ"
     gene            complement(4211..4960)
                     /locus_tag="VIG33_17940"
     CDS             complement(4211..4960)
                     /locus_tag="VIG33_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY8272780.1"
                     /translation="MARNQDKNIFLIVSGNSSRITVMSDTLNKNFPNCSIFHATDWFD
                     TKYKIDNVFPKIVFVDEYLPKSSGLEVVAKIYKEKNYSDLHIVILSVIVDSELYPPNG
                     RIHYLTEPEREASVVECVSRIVAPKETAKVPEYTLKHLEPGELLFKEGDSPSTVYIVK
                     NGTLKAYSDTLACGRVILGEISAGEFVGEMGHFNNEPRSATIEALTEVDVVEIPMSNL
                     DSVIFSKPSWARALVKTLSQRLKRANKVLTG"
BASE COUNT         1609 a         1132 c          972 g         1493 t
ORIGIN      
        1 gtaatcataa gtaatttctt ttccagagac acaagccact tttttagcta tagcgtccac
       61 gctttggacc ctgtctcgaa tccaagatac cccattgggc atgagttcgc tcataggctt
      121 tctggttttt tctttgggta caacccctgc tcctaccaat gtccacaaag gttgatagaa
      181 atgatattcc gctggctcaa tcagaccgat atctttaccg ttcatttttt tattaaggcg
      241 agcagcgacg gcaacaccac cagttccagc accaagtatg agaaccttaa aatgagaaat
      301 aacttgctcc ttagttaaaa atttaatttc gactgtcttt gcttatatag gccctcattc
      361 tttagactaa actaactaag ataagaggcc gtttatcgac ttcaatcaaa acgattttta
      421 cacaaaattc aatgtaaaaa taactctcta tataagaata agagcggggc attaaacaca
      481 ttagagcatt taataactaa gtcgttttat tggatttttc ttcgttagaa tttttcacgc
      541 gcttggaaag agtttttagc aatgctttgg accacgcagg tttagaaaat aaaatagcat
      601 ccagactaac gcttggaatc tcaatcaact cacagtcagt gatactaatg acatctgcag
      661 agcgagcctc accgttaatg taggccattt cgccaacaaa ctctccagga taaatggttc
      721 cgagtaatat ttcatttgcg ttctcagtct tataagctct gagttctcca gacttaagga
      781 tgtaaacgaa atcagccgtt tcgccctctt tgattaaata ttcgttgggg gacaagaaac
      841 gcaaatgata gattgattta tcttcatgag acacccaatt cagcgcacgt gtaatgtgat
      901 tattaaatac tttcacttgg ccactacccg ataaaaattg tacttgcccc gtcacaactt
      961 catcgatgaa ccgatcttca tcagcaacag gagctaaaat aattacggca gcgcgatctt
     1021 ttctctttaa aatattataa gtcaaatcca cagcgctcaa tttcgaaatt tgagaatcta
     1081 caaccactac gtgaggaggc acattctcac atttaaatag cgcttctaca ccatcaacgg
     1141 ctgtaaatac tgtggcgttt tgcacatgag ctttgattgt ttcctcaagc tgagtattca
     1201 attccgcatt tggggtgact actaagaata ttcttttatt ttccaactct gccatgtacc
     1261 tatgctattt caagtctatc caatagcaaa caaaatcaac caattcagac cttaagcaat
     1321 cgattttttt caactttcca tttaatcctg attcattttg agacaaaaag tgaatcatat
     1381 tagttctcaa aaaatgaact aaaaatgccg atatactatt catgacaaaa tcaaataagc
     1441 gtattggagt atttgcaatc ctggcaggtt gggctctcct tattatgttt tttcagaata
     1501 tgacatttat cgagttctcg accctaaatc tccccttagt agatgaggac tcccggcaag
     1561 accaagctcg agaactcttg ggcaactact ataaagggag tctggctcaa cgattcgaag
     1621 gagagcagtc actaaattat ctggtctata aaaaaattga tgcatcaatg ccagatcgct
     1681 ggaaaaaccg acttcctgaa atcaccgagg ccctgatcgc tgaaagccaa aagcaagaac
     1741 tagaccccat ctttgtttta gccgtaatcc aaactgagag taattttaac actgacgctc
     1801 gtggcacatc aggggaaatc ggcctcatgc aaatccttcc taaaactgcc gagtggattt
     1861 cgaagaaata taaaatgccc tggaagggat cccgttctct cttcgatccc atcgtcaaca
     1921 ttaaaatcgg cgtcacttat ttcgcacatt taagagatga gtttgaaagt cgtgcctatc
     1981 actatcttcc agcctacaat atggggccga gaaatatgcg aaaagtcagt cgtactattg
     2041 gcagtatttc cagtactgga cgaatcataa aacgtgatta tgccatgaga gtcatgaaaa
     2101 attacaccaa tatctatgag caaatggtag tagaacaaaa agaactcatc aaacttgcta
     2161 aaaatcaaaa tagttccaag atctcagaaa aactcagccg gtaattatgt tttttataga
     2221 tgagtacaag ttcgatactt tctttatgca aaagcacatt aaacatctat aaacaattgt
     2281 gaacttggta tgatcttgga taagaagact ctctaaaaat ggaggtcttc aatgaacaag
     2341 cttaaactat cgtttcttgg ctttatttta tcggcaatat acttactcac agcatgtacc
     2401 ggaaatttag ccaaagacct cgagacggat cgtgatcatg aaaaagattc agatttttat
     2461 ctagccgcgg tcgaagacca aaatgcctct ccagatctta tacgcaaaaa gctcagttat
     2521 ttttttctcg tagaacaatt caacgtaggt ataaatggtt cgatgctcgc tgccattaaa
     2581 agcaatcccc caggcggaat ccttttttgg aatggcaaca atgcagatgc taccttgctc
     2641 cgtgaatctg tgcgtgccta ttctcaacag gctgaagctt cgaacctgaa ccctcttttg
     2701 ttctctacgg actatgaagg tggggctctt caccagtcac caaatggaac taagactccc
     2761 ggagtccagc gctttactaa aggcttctca agtctagctc atccccgctg gttgggtatt
     2821 tcaatgaaag aatacggcac agaactctgc atcttacatg gtaagattat atctcaggaa
     2881 cttaaatcag tcggcatcaa ttatccctta tcggtagtaa gtgacctagc tacgcagact
     2941 ttgacttttg ctcgcgctat ttcaaaaaac cctgaagaga ccagcctatg cataacaaaa
     3001 atatttgagc aatttataaa gactaaagat attatttttg taacgaaaca ttttccaggt
     3061 cttgggctca caaagggcga tacccatgag ggaactgtga cggccactac aactgatgag
     3121 gaaatcctct ctgctcattt aaaacctttt gctgatctta tcagattttc aaaatccaac
     3181 gctgcggaaa aactattaag tattatgaca actcacgcga aattcttggc ctatgatgga
     3241 gatcacgtaa ccacagaggc cccaaaaatc gtcaccgagc ttcttaaaaa gaaaatgagc
     3301 tttgaaggac ttgtggtcag tgatgcaatg tggatggggg aatatggaaa aatgaaaagc
     3361 atgcaactta tgtccgtcta tctaaatagc ttcttgtctg gaattgatct tttaatgatt
     3421 cccggcgtac gatttgccga atctgtaaac tatttccgta aagtctatga tggcagtctt
     3481 acagcagaag aaaaagaagc gttgacggat cgcacaggtc taagctggca agaaacacaa
     3541 gaaaagttca tggctcgcat cgcgcaaagc ttaaatgctc atgatactgc aagaagcaaa
     3601 atcaaggctc cgcatattta tattcagaaa agtgaaactc cttccaacct tacgagcgct
     3661 gaccgcgccc gttataatga gatcttgagt caactttcaa ttcacggtaa ttctttacag
     3721 aaagattaaa aaaggatttc tgtggataag aaaaagttga ttgcccatat tcgagcagaa
     3781 ttggaaaagg atcttttgat ccttaaggaa gttgcccgcg taactcgcga agcagcaact
     3841 cacgaagaga gcaaaccaga aaatcagtat gacacccgcg cacttgaggc ttcatatctt
     3901 gcgggtgctc agtccaagcg tatcactgag actgaggacg tcttggtcgt ctataagcat
     3961 atcgagccga agtatttcac ggaacaagat tcaatctcag ccaccgcact agttgaagcg
     4021 gaactcaatg gaaaaaaaag ttatttcttt gttatgtcta aagggggcgg gctcactgtc
     4081 acttttgaag gtacaaagat tcaagtcatc acgccaggaa gccccttagg tggagcttta
     4141 ctcggactta aagtgggcga cttagccctt cttgataaag gatcacagat cctagaatac
     4201 gaaatcttat ctatccagta agaaccttat tggccctttt gagcctttgt gaaagagtct
     4261 tcaccaaagc tctcgcccat gatggcttgg aaaaaataac actatctaaa ttactcatgg
     4321 gaatttccac cacatcaact tcagtcagtg cttcgatcgt ggccgatctc ggctcattgt
     4381 taaaatgtcc catctcacca acgaattcgc cggcgcttat ttcccctaga atcactcggc
     4441 cacacgcaag agtatcagaa taagccttta gggtcccgtt ctttactata taaacagtgc
     4501 tagggctgtc accttcttta aaaagcaact cgcctggctc caggtgtttt aatgtgtact
     4561 caggaacttt agcagtttct tttggtgcta cgatccttga gacgcattca accacacttg
     4621 cttcccgttc aggctctgtc aagtaatgga ttcgaccgtt aggagggtaa agctcagagt
     4681 cgacgatgac cgacagaata actatatgaa ggtcactata atttttctct ttataaatct
     4741 tagcgaccac ttcaaggccc gagcttttcg gcaggtactc atctacaaag acaatcttgg
     4801 gaaaaacatt gtctattttg tatttcgtat caaaccagtc tgtcgcatga aaaatagagc
     4861 agttcggaaa attcttatta agagtatccg acattaccgt gatacggcta ctgtttccac
     4921 ttactataag aaaaatgttt ttatcttgat tacgtgccat gttgtattac ctcttccata
     4981 ttatctactt gatacacaac tgacgtactg cttttgtata tatgtatatt atcctcctcc
     5041 ttatgttcat actcaagcgc ctgtcaaaag agacgtcagg ttgctttaag gcgcgtcaaa
     5101 tcgagaaaga atacatgctt taaaaaaagt ctgaatgtct atttctgaga aaatttatta
     5161 aaactacaac tcaggctaaa gtcttctttt tgattcaact ccgagt
//
LOCUS       DATBTE010000472         4930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1090485, whole genome shotgun sequence.
ACCESSION   DATBTE010000472 DATBTE010000000
VERSION     DATBTE010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090485"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VIG33_17945"
     CDS             <1..228
                     /locus_tag="VIG33_17945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272781.1"
                     /translation="SASFHEKAFLAAIVAIRAKDQFNSHHINEITFSIFAPRVSLKWP
                     RWPFYSKLKDGRTLAPILSAFKALSLQNAKK"
     gene            complement(338..739)
                     /locus_tag="VIG33_17950"
     CDS             complement(338..739)
                     /locus_tag="VIG33_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272782.1"
                     /translation="MKKTIFAMTLLAGFSTHAAQVPVFKCNVPVKTSTPTVSVNVSIS
                     DNESVDYITVQLNDKTATNLFMQLEKGSLQKQITAGGVTTLVLGDQFSQGADGVIRDG
                     GVLGLGTDTSGKWSGMLAAKGNVYPLECTKL"
     gene            1058..1675
                     /locus_tag="VIG33_17955"
     CDS             1058..1675
                     /locus_tag="VIG33_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272783.1"
                     /translation="MKRYVLAAFTFCLIIGFQNCSPHQFTDVNNSNALSNPAEVTPAE
                     KVDVSQTEIVEIPESPYLESLLQSDIIAINPTSTFASHHLTINVKTGVVHVMDQNNET
                     LAGIQYCLSSSDVNELQTILTSSKLCENQAIIDEQTNCTANYQFPYARLQVLDTKISL
                     GESTSGCHKGPDLCGEQRDLLQGLIAHIQGDLASKKCEFQVVQQQ"
     gene            complement(1689..4403)
                     /gene="alaS"
                     /locus_tag="VIG33_17960"
     CDS             complement(1689..4403)
                     /gene="alaS"
                     /locus_tag="VIG33_17960"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY8272784.1"
                     /translation="MKSSEIRNAFINYFKRNGHTPVPSSSLIPENDPTLLFANAGMNQ
                     FKNTFLGLEKRDYTRAVSSQKCVRAGGKHNDLENVGFTARHHTFFEMLGNFSFGDYFK
                     KDAIHYAWEFLTQELKIPKEKLYVTVHISDDEAADLWHHQEGIPHDRIFRFDKDNFWK
                     MGDTGPCGPSTEIFYDHGPKAGKESDPFKGIAAGEDRFVEIWNLVFMQYFENPPGTLT
                     PLPKPSVDTGAGLERLVAAMQGKFNNYDTDLFAPMIERACKIGGFEYITDVDVLTKNP
                     EIRERTAAIRVMADHCRATGYLIADGALPANEGRGYVLRRIMRRAIRYGHKLNDKHSF
                     LPSMVETMIDFMGDVYPELSIRKVHILGAVKDEETRFLATLDQGSHILNEEISQAKAK
                     SLKTLSGEVLFKLYDTYGFPVDLTGLIAAEKGLSIDEAAFEKEMENAKVKARASWKGK
                     GMQADEAHILKFAQDRLSKNGPTKFTGYESTTGQCEIIGLSDGTKEVQSLKAGQTGLL
                     VLNKTSFYAEGGGQVGDQGVMKSPSSTVNVHNTTKSSEVHLHHVEVESGEIKVGNSML
                     IQVAENPRRNTMSNHSATHLMHSALRKVLGPHVTQAGSLVDAQRTRFDFTHNKSLSLE
                     EISQVENLVNTEIGKALEVKAETMNHKEALEKGAMALFGEKYGEVVRVLTIGDFSCEL
                     CGGTHVKNTSQIRMFKVISESGVSAGVRRIEAITGDLAVQYALYSIQQNQQARGVAGV
                     EPAWSHFVTQAPLESSLVSWIESKKNEIKNLEKEVKKLQGGQINIDDLVDKAQKFNTK
                     TGAAKLVMADLALDDREVLAQVTDHLKNKIQKGIVVVVGRGEGLHPVIVSVSKDISSE
                     YSAGNLLKEVAAVMGGKGGGRPDFAQGAAPDRTKIAEAFRKVQQILSL"
     gene            complement(4390..>4930)
                     /locus_tag="VIG33_17965"
     CDS             complement(4390..>4930)
                     /locus_tag="VIG33_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEY8272785.1"
                     /translation="PAQSPYTHRLYAKKKVMDLVALRDHSEKELRTKLRTYFTRNFQY
                     RQRLAKKQGRDLSEEFASQQQEISDAIDGAIEFAKNHQWICKPEDLSEKMATMLHRKK
                     KGISYINNYLKEKGLPSIPQNRDLELEKALALVKNKYSDFSKLSSKERKKEQARVGRF
                     LAARGFDSETVRKVIYEKL"
BASE COUNT         1320 a         1117 c         1036 g         1457 t
ORIGIN      
        1 agtgcctcct tccatgagaa ggccttcctg gccgccatcg tggcaatcag agcgaaagat
       61 caattcaatt cacatcatat caatgaaata actttttcta tttttgctcc ccgcgtatcg
      121 ctaaaatggc cacgatggcc attttacagc aaactcaagg acggtcgaac tctcgcgcca
      181 attctctctg cgtttaaagc cctctcgctc caaaacgcta aaaaataaaa aacccacatc
      241 tcaaccaaag atatggtcat agatgtgggc cctagaaaat tgcatacttc tttttctatt
      301 caatcctctt atttaaaaga aatttaataa aagaaaatta aagttttgta cattctagag
      361 ggtatacatt tcctttggcc gcgagcatgc ctgaccactt acctgaagta tcggttccga
      421 gacctaaaac tccgccatct cgaataacgc catcagcgcc ttggctgaat tggtctccta
      481 atacgagagt ggttacgcct ccagcagtga tttgtttttg caagctgccc ttttcaagtt
      541 gcatgaaaag gttagtggct gttttgtcat tcaactgcac agttatgtaa tcaactgatt
      601 cgttatctga gattgataca tttacggaaa ctgtgggagt ggaagtctta acaggaacat
      661 tacatttaaa aactggcact tgcgccgcgt gagttgaaaa gcctgccaaa agtgtcattg
      721 caaaaatcgt ttttttcatt cagaacccct tgttaaattt tggacgaggc tagtgcaact
      781 ccctaaaggc agcaaccaaa aatttatgac attatattca caacagaaga cattatttca
      841 cattgagact tatctttagt tattaaatgt atgtacgtat ttgtaaatgt ttgtaaatgt
      901 ttgttgaaat gttttgtcac ggattgagaa ttgctaatca ctcattagac aacgtcaagt
      961 ttctacacag tctaagcgaa tacaataaga ttttagccgg tattaatttt gcagcgtttt
     1021 cttttagcac cggtgaatct ttctttttgg gggattaatg aaaaggtacg tacttgcggc
     1081 ttttactttt tgccttataa taggctttca aaattgcagc ccacatcaat tcacagatgt
     1141 taataattca aacgccctta gcaatcctgc agaggtgacc cccgcggaga aagtggacgt
     1201 gtctcagact gagatcgttg aaattcctga aagcccttat ctggagtcgc tgctacaatc
     1261 cgatattata gccatcaatc ccacttctac ttttgcttcc catcacctaa caattaatgt
     1321 gaagacgggt gtggtgcatg taatggatca aaataacgag actctagcag gaattcaata
     1381 ctgtttgagc tcttcggatg tgaatgaact ccagaccatc ctcacctctt cgaaactttg
     1441 cgagaaccag gctattatag atgagcaaac gaactgcacg gcgaactatc aatttcctta
     1501 tgctcgtttg caagtcttgg atactaaaat ttctttgggt gaatctacga gtggctgcca
     1561 taaaggtcca gatctctgtg gagagcaaag agacttgctt cagggactca tcgctcacat
     1621 tcaaggtgac ctcgcctcta aaaaatgtga gttccaagtc gtccaacaac aatagctcta
     1681 aggtaagctt ataaacttaa gatctgttgg acctttcgga acgcctctgc tatttttgtt
     1741 cggtcaggtg cggccccttg agcaaaatcg ggacgtcctc cgcctttacc acccattaca
     1801 gccgcaactt ctttcagcag gtttccagcc gagtattcgc tagaaatatc tttactcaca
     1861 gaaacgatga ccggatgtaa gccttcccct cgccccacca ctaccacgat tcctttttga
     1921 atcttatttt ttaggtgatc ggtgacttga gccaacactt cgcgatcatc cagagcgaga
     1981 tctgccatga cgagctttgc cgctccagtt ttggtattaa atttttgggc cttatcaacc
     2041 aaatcatcaa tatttatctg tccgccctga agcttcttaa cctctttctc gagatttttg
     2101 atttcatttt tctttgattc aatccaagaa acgagagagg actctagcgg agcctgagta
     2161 acaaagtgag accaggcagg ttccacacca gcaacgcccc gggcttgttg attttgctga
     2221 atgctgtaca aagcatattg aacggcaagg tctccagtaa tggcctcgat acgacgaacg
     2281 ccggcactca cgccgctttc agaaataact ttgaacatgc ggatttggga agtgttctta
     2341 acgtgggttc cgccgcaaag ttcgcaagag aaatcaccta tagtcagaac tcgaaccact
     2401 tcgccatact tttccccgaa aagagccatg gctccttttt caagagcctc tttgtgattc
     2461 attgtttcgg ctttaacttc cagggctttg ccaatttcgg tatttaccag gttttcaact
     2521 tgagagatct cctcaaggct caaagacttg ttatgggtaa agtcaaaacg agtgcgctga
     2581 gcatccacca aagatcctgc ctgagtcaca tgaggtccca agactttgcg aagcgcggaa
     2641 tgcatcaagt gagtggcaga gtgatttgac attgtattac gacgtggatt ctctgcaacc
     2701 tgaatcagca ttgaattgcc gactttaatc tcgccagact caacttctac atggtgtaaa
     2761 tgaacttccg aagactttgt cgtattatga acattgactg tggaggaagg ggatttcatc
     2821 acaccttggt cgccaacttg accgcctcct tcggcataga aagaggtctt attcaaaaca
     2881 agcaaacctg tttggccagc tttgaggctt tgcacttctt tggttccatc agaaagtcca
     2941 atgatctcac attgacctgt cgtggattcg taaccggtga attttgtagg gccgtttttg
     3001 ctgaggcgat cttgggcaaa ctttaagata tgggcctcat cagcctgcat tcctttgcct
     3061 ttccacgaag ctcgagcttt cactttggcg ttttccatct ctttttcaaa cgcagcttcg
     3121 tcgatggaca ggcctttttc agcagcaatc aaaccggtca aatccacggg gaatccgtag
     3181 gtgtcataca atttgaaaag cacctctccg gaaagagttt taagactctt ggcctttgct
     3241 tgggaaattt cttcgtttaa gatatgtgag ccttgatcca aagtggctaa gaagcgggtc
     3301 tcttcatctt tcacggctcc taaaatatgg actttgcgaa tcgacaattc agggtagaca
     3361 tcgcccataa agtcgatcat tgtttctacc atcgaaggta agaaagaatg cttgtcgttg
     3421 agtttatggc catagcgaat ggctcgtctc atgatacggc gaagaacata accgcgtcct
     3481 tcattcgcag gaagtgctcc gtcggcaatt aaataaccgg tcgcccggca atgatccgcc
     3541 atcactcgaa tcgccgccgt tctttcacga atttctggat ttttggtaag cacatccacg
     3601 tcggtgatgt attcaaaacc gccgatttta caagcgcgct caatcatcgg agcgaaaaga
     3661 tccgtgtcat agttattgaa cttgccttgc atcgctgcaa ccaatcgctc aaggcccgcc
     3721 cccgtatcta cagaaggttt tggcaatggc gtcagtgttc ctggagggtt ctcaaaatat
     3781 tgcatgaaga ccaggttcca gatttctaca aagcgatctt cacctgcggc aattcctttg
     3841 aaaggatctg actctttgcc ggctttaggg ccatgatcat agaaaatctc agtggaaggc
     3901 ccgcaggggc cggtatcgcc catcttccaa aaattatctt tgtcaaatcg gaagatacgg
     3961 tcgtggggga tgccctcttg gtgatgccag aggtccgctg cttcatcgtc agagatatgc
     4021 acggtgacat aaagtttttc ttttggaatt ttcagttctt gagtgaggaa ttcccaagca
     4081 taatgaatgg catctttttt aaaataatca ccaaaagaaa aattgcccag catttcaaag
     4141 aaagtgtgat gacgagcagt gaaaccaacg ttttccaagt cattgtgttt accgccggcg
     4201 cgcacgcatt tttgtgaact cacggcgcga gtgtagtcgc gtttttcaag acctaaaaaa
     4261 gtatttttaa attgattcat tcctgcattg gcgaaaagca atgtgggatc attttcggga
     4321 attaaagaag aggacggtac aggagtgtgt ccgtttcttt tgaagtagtt gataaaagcg
     4381 tttctaattt cagagctttt cataaatcac ctttcgcacg gtttcagaat caaatcctcg
     4441 agcagctaag aatcgcccca cacgagcttg ttcttttttt ctttctttag aggagagttt
     4501 tgaaaaatca gaatatttat tcttcacaag agccagggcc ttttcaagct ccaaatcacg
     4561 attctggggg atggaaggca ggcctttttc tttgaggtag ttattaatat aactgatgcc
     4621 ttttttctta cgatggagca tcgttgccat tttttcagat agatcttcag gtttacagat
     4681 ccattgatga tttttcgcaa attcgatggc tccatcaata gcatcgctga tctcttgttg
     4741 ttgagaagcg aactcttcag ataggtcacg gccttgtttt ttagccagcc tttggcgata
     4801 ttgaaagttg cgcgtaaaat aagtgcgtaa tttcgtacgg agttcttttt cagaatgatc
     4861 gcgcaaagcc acgagatcca tgaccttctt tttcgcataa agtctatgag tataaggaga
     4921 ttgtgccggt
//
LOCUS       DATBTE010000473        21483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_680014, whole genome shotgun sequence.
ACCESSION   DATBTE010000473 DATBTE010000000
VERSION     DATBTE010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 21483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21483
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680014"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..326
                     /locus_tag="VIG33_17970"
     CDS             81..326
                     /locus_tag="VIG33_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEY8272786.1"
                     /translation="MKRQFIRSHVHINIHPLERWIRIIAGAILVVLAFPGGQINGWYL
                     LGVIPLLTGIIGFCPLYYAFGINTNRWARVKVDHTKK"
     gene            780..1904
                     /locus_tag="VIG33_17975"
     CDS             780..1904
                     /locus_tag="VIG33_17975"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY8272787.1"
                     /translation="MKYCIKNFIAILFVHFLFIAPSYGEAVDSPENWIIEKIECSGNE
                     ITACSIIENEIYLRAGDKVNEEQIENAQLRLKTLGLFYDVQMSLQKGVIRGQVILVVQ
                     VQERSAHYMNLSGYSYKSNTASGIIGIGDRNLFGLGKRLEVDVERSFDQNRQSFNAMF
                     EDPNIFGSSKYFSSVHLLYFKVDNDDIQYKSSILSIALGRRIFDFSYIKLIARGGLSE
                     FSTSGVSNGEYKSLGEGVEYGWNTLDDLYFPTSGTTLAAGSYFSDIGARYYLSSSFIN
                     SLYKDYYLTLEGGLDLDKFDSDSAFNGTLSFRPAIAKYFTFHRQGQRIPDKMKIFLGA
                     DAFNLPATSSRIEFNGTLGVSYLIDSFGKIDFTVSFQDMR"
     gene            1961..2581
                     /locus_tag="VIG33_17980"
     CDS             1961..2581
                     /locus_tag="VIG33_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272788.1"
                     /translation="MLQPLQKNEAAIQIKGVEVPREQLKRHLKSGLHSLFLFELAATL
                     KSSSKSELLFKEKYRWKVYYDLWDEVYRVTVQGDDNTESKMEFKTLENFFTALENPQF
                     RNLQTLVVVKNFPKNGTLGIRILLNPVSQETVERVKKWVHQRRVLGSSGAVIFGAKSG
                     EATQEGTPRFLSLFNEILGYEMNDAKQDSAWAFAGVAPLLIQETKQ"
     gene            2578..3702
                     /locus_tag="VIG33_17985"
     CDS             2578..3702
                     /locus_tag="VIG33_17985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8272789.1"
                     /translation="MKRHRVRLWLILSLMTMVAIPLAGAYFQIQDVLNSSFSMGSEAE
                     SIEALEMGQTALKELAKKSPASEQKYHEQFLKIQELKLSLAEHGLIRQGLVNSYTFYF
                     LVSFILMMLISIGFAVFLSRKVARAYDQSFEELVRQRDRSQFLEGMARWQDVAKQLAH
                     EIKNPLQPIGTWMRNLVAVFPNQKKPEDFSMLLDEAAESIHQEIHHLQSLVDAFTQFS
                     RLPEAQFNKVDLGLFVREFQQRYQESWDVDWQLAVTSKEALIQADVKLLRQVLANLIT
                     NAVEANCKDRIVVHIQVAQKNGHVELVVENSGKVIEAALKEKIFEPYFSTKSAAANFG
                     LGLAIVRKIVFDHGGDINVEDCFTGARFKLTFPLIGEGHG"
     gene            3695..5059
                     /locus_tag="VIG33_17990"
     CDS             3695..5059
                     /locus_tag="VIG33_17990"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY8272790.1"
                     /translation="MDNYCRILLIDDDKNIRRSLQVALQNKGFEVHLAQDAQQAFQTL
                     QKESFDILVVDVVLGEFSGVDFFSRIRQQYPETPVIFMSGEATLTEVAKLMRLGAADF
                     LEKPFTEERLILSIERSLEFSRLQNRIQYLEKGQTPKEDEIYGDSNPIREVRELISRV
                     ARSNAHILVTGESGTGKELIAKQIHLQSKRSNEAFIKINCSAIPETLIESELFGHERG
                     SFTGATQSKKGYFELANKGTLFLDEIGDMSLAAQAKILRAIQSGEIQAVGSISVRKVD
                     IRLIAATHKDLKKAVQTGEFREDLYFRINVVPIHLCPLRERSEDIPLLAEKLIRDIAL
                     ENSYIERTFSADALELISRWSWPGNIRELRNFLERLLIMGGAGLILPEDLTMPFFFKE
                     TQESLHPVSLKEFRDSAEREYIVGVLKKVGGNISKTAELLEIERTYLHRRLGHFKIEK
                     REYL"
     gene            5189..5830
                     /locus_tag="VIG33_17995"
     CDS             5189..5830
                     /locus_tag="VIG33_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272791.1"
                     /translation="MKLKTILTAMILLTTHFGWAAVKDDESTDTLSIEVPKHQVKVGL
                     SAEPIWLLVGGVGAKFEYFVTDHISVGINGVYIPEHQIETSSSKDQSLTSESYRWSMN
                     EINLGSNIMLTGSLSTNGVYLNPAVGYQNTRISDYGVLKLKGSLSSPQARLTAGYQFV
                     NPANNLRFALGAGLRMIQDSDILVKDDTGEEVYRQKTSGLGGLALDCHLGYIF"
     gene            complement(5827..6690)
                     /locus_tag="VIG33_18000"
     CDS             complement(5827..6690)
                     /locus_tag="VIG33_18000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY8272792.1"
                     /translation="MDVDNKQTSAPDSSAVRVALWRAMHVQIDPLPHVLEDEIGLQLV
                     NPDAGWRQRQDMHPQGTRGYRAGIVARARLIEDLVVEKLSHGIHQYVILGAGLDTFAQ
                     RRPEIASQLQIFEVDQPGTQEWKRRRLIELGFDTPENLHFVPVNFEAGESWKEKLIAN
                     GFDASQPAVIASTGVSMYLTKEANSMTLRQIASLCPGSILAMTFILPLQLIDPAERTQ
                     LQMVYERARAAGTPFISFFEPSEIMALARETGFKNTQHISRDGIIQRYFSGRTDGLQP
                     SSGEEFLIATT"
     gene            7686..8453
                     /locus_tag="VIG33_18005"
     CDS             7686..8453
                     /locus_tag="VIG33_18005"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY8272793.1"
                     /translation="MDSLERWSFMKVFVLSYILMLAPLVGRADLFVDDHVTRTVMTEN
                     ERHLPVVQVAKSSGILLGESLLLTAQHTTKNWQSYSYQGATMFYGPHNDHYYFKFESI
                     IEEDAALDYAIVRISWKNGVIPPGLQWVKSIVMPGEIKLGVIHTMDIFTLGSPWDKIP
                     NPMKSSGSKIVILPDSQIKYPLLFNAGVIEGNSGGPIFTADGKLLGIVTGGPHKLGEP
                     GWEGNDPNKIESWNWGTPIQNIFKISKVLQELFPRGE"
     gene            complement(8683..8769)
                     /locus_tag="VIG33_18010"
     tRNA            complement(8683..8769)
                     /locus_tag="VIG33_18010"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8733..8735),aa:Leu,seq:caa)
     gene            complement(8872..9525)
                     /locus_tag="VIG33_18015"
     CDS             complement(8872..9525)
                     /locus_tag="VIG33_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272794.1"
                     /translation="MKKITLMTMISLTLTSMSLAQGTATTKLRKTAPPTYNYDPDTLV
                     GPSPYMVDGFNLGFEYLSVNSLQSDIKNKLTGNLEKGLKEDASKIPKQLGFKMGYKQI
                     PRGGMGFDLNLSILKLEKRPEGTSDLTNVVPAVNFIVAAPKYAYAAVGVNTSIVAGDD
                     NAQHSPHIGYQAGGGIVLQKNFNFEILYSWINQGIESQTALTEERMTSTNARLIYAF"
     gene            9883..11094
                     /locus_tag="VIG33_18020"
     CDS             9883..11094
                     /locus_tag="VIG33_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272795.1"
                     /translation="MNKYFYILAALSIVPNLSLAQAGNFDAEVDQELDQMYANKQAGT
                     VASPAPTRGSVGGVAAQGSQPIYILNQATPTAATNTNVQTQTAQVQRQPVTLIESTPL
                     TESKAELMRKSRQDAEVQTEQKIVEKLEYSRLEDEKKRAEVLFGNKFEQMQGNTTQIK
                     AENSNVSVNTQQQATQVPVVVQAPVTPIEPKENFRDVVREELHNAMKVEEPIEVAAVE
                     TKYFAAIAGIGDYPDVRNVRGNYAFGAAFGTKYDQLIVEGSFLLSNYTVEKIDCNCVS
                     YYPTLVDTNQYQGAVAAKYQMMSGLVRPVVGGLAAYSYRKYNWTNVVNAYPYSNYGTS
                     SDSHAVDLGVTAGVDLELSKKFALGFEYKYMFNLSSRVNNQSIVTGPQYGTPIEKLSY
                     YTMALAAKVNF"
     gene            11199..11870
                     /locus_tag="VIG33_18025"
     CDS             11199..11870
                     /locus_tag="VIG33_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272796.1"
                     /translation="MFLVLTLSVLSGIFSLAATPAVISQSVGRVGERIVTSREVQISA
                     VLTRALFPIKEAKPSVNLYEVHLGDSAFMGEVTALLLEVVVDKEAESFSVAKVSEEEL
                     QQSLRKIEKVVEGKTYWESLEVSPAELKRFVLQKLTAKAFIQFKTNSMVGVVSDAEAL
                     AYYEKNRLKFGDLPFSSFKENIKTFLAQQQLEERSRTWFEIIKRKYKVRNILAENMKN
                     KVGPK"
     gene            11867..12310
                     /locus_tag="VIG33_18030"
     CDS             11867..12310
                     /locus_tag="VIG33_18030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY8272797.1"
                     /translation="MSFKMRPLLADDEAQVLQLVADLYRLHPYLEGFQWPSETLRAEF
                     QSTDGWGVFKGPRLVSFVLYRVTPVAWEISVVASHPEFWKQSLTRSLLGHLIIIKPLD
                     RELWLEVHCENTRAWRLYESLGFVRTGLRENYYPNQGTAWLYTLR"
     gene            12433..12987
                     /gene="rimP"
                     /locus_tag="VIG33_18035"
     CDS             12433..12987
                     /gene="rimP"
                     /locus_tag="VIG33_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY8272798.1"
                     /translation="MTESNPSWMEKVEKMAEEATSGEGCFLYDIEFIGVGNKGRTLRL
                     YIDKDTPQAVPDSEEGQGVSITDCSNVSKALNLLLDVEDIIPGESYNLEVSSPGLERI
                     LRKPWHFEKAVGKKIWVKTSKSFESFGVTDKKLKAAKQIDEVLKAFDGETLSFQVKDV
                     EVNIPLSAVEKSKVLFVLTKGQKK"
     gene            13006..14403
                     /gene="nusA"
                     /locus_tag="VIG33_18040"
     CDS             13006..14403
                     /gene="nusA"
                     /locus_tag="VIG33_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY8272799.1"
                     /translation="MADNMFTDLSRVIEQVGKDKGIDKQVVVDAITQGMLVAARKKYG
                     TYREIEATYNEETGEVELHQFKEVVPREKYIDEEVEIPMDEALKLDPQAQEGDSIGIK
                     MDAGELGRIAAQTAKQIIMQKVRDAERSIIFNEFEERKGEIASGIARRVEKGAIVVDL
                     GRTEAYIPPREQIPGESYKPGDRIQGYLSEVRQTTRGPQIIMSRADERYLMKLFEMEV
                     PEIYDGIVEIMSAAREPGQRAKIAVRSKDNSVDPVGACVGMKGSRVQNIVQELRGEKI
                     DIIPWDEDVTRFACNALAPAEISRVFVDEANKEMEVVVPDSQLSLAIGKRGQNVRLAA
                     KLTTWKLDIISESSASSRTAEAIFNLMLLPGMSQTMAQNIFQSGFGSFQSVANASVEE
                     IMTIPGYDDPERAEKLMGEAKNLILKYEKEGLPIPTAPSAVKESKASGSAKEQADLRL
                     KQELQKLDAQAEDKE"
     gene            14472..17396
                     /gene="infB"
                     /locus_tag="VIG33_18045"
     CDS             14472..17396
                     /gene="infB"
                     /locus_tag="VIG33_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY8272800.1"
                     /translation="MSNPKVFEFAKEIGLTPLALMDKIREWQIPVKSHMSELEPEVLE
                     QLKVKLSGGNKSTEEAKSKRTTTRKAPAKKVAAPAAEAAAATANVTAKSTVVRRKATK
                     EAEDAEITAQLKSAADKAEAEETAKAVAKAVPKKAAPVVEKEVPAPKVVVEMPPAPVV
                     VAPPVIEAPVREAAPVVEVPVAPSSAPTPPVVAPPTPAAAVSAATSVGVARKKEVTVG
                     SSGVASAATAAPSIGRRNIIGRMDLSRVAQQAPQRSAERSTSAGDRQGGFNSGAPRTG
                     GFSGPARTSTQNRNIRPGFVAQAQPDVPVEPIESRHGKFDDKRKPKFSPVPVVAAGPS
                     AKEREQEEQISNFNPVEFRKREMVFQPKKKKGMLERAGMKTQITTPKALKRIIKVNNA
                     MKLSDVAMEMGIKAPQIIKVLMQNGVMANMNTLLDFDTIALIVPEFGWEAQNVFKTAD
                     DMATETAFGNLDAAMISRPPVVTIMGHVDHGKTSLLDAIRKANVAAGEAGGITQHIGA
                     YSVTLDDGSLVTFLDTPGHEAFTKMRARGANATDIAVIVVAADDGMMPQTAEAINHAK
                     AAGVPIIVALNKIDKPGANPERIKQQLTELQIVPEEWGGNTIFVEVSALKKTGIKELL
                     ENIHLVAELQDLKANPERSGTGLVIEAKMEKGKGNVATLLVKDGTVKVGQHIVAGTIK
                     GRVRSLTNDKGERVDSVGPGIPVEVLGLEGTPSAGDRFDIVKDEETAVEVSEMRAEQA
                     KKAEVVPASKMSLEDIFAKATRGDVKDLPIILKADVHGSLEAVSGMIQKLATAEVKTK
                     IIHSAVGGISESDVLLANTANGIIIGFNVRPDGGAQTAAKRSGVDIRTYSIVYELVDD
                     LKKAMSGLLTPDVVEKVMGRAEVRNTFTVPKIGTIGGCFVTDGKIQRSNMARLIRDGK
                     IVYEGKISSLKRFKDDAKEVASGFECGIGIENFNDLKVGDAIEAFIKEEVARELTYEA
                     NP"
     gene            17396..17803
                     /gene="rbfA"
                     /locus_tag="VIG33_18050"
     CDS             17396..17803
                     /gene="rbfA"
                     /locus_tag="VIG33_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY8272801.1"
                     /translation="MKKEGDGGRRIARVEREVQQTIARFLISGFKLPLPGIVTVARVQ
                     MPGDLRTAKVYISVIGEPSQLDEVVKLLQGRAFEIQNYLGKELKMRYCPKLSFYADFD
                     TQQILKVERILQDLEAERNQSKKVKDDSSEEDS"
     gene            17800..18711
                     /gene="truB"
                     /locus_tag="VIG33_18055"
     CDS             17800..18711
                     /gene="truB"
                     /locus_tag="VIG33_18055"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY8272802.1"
                     /translation="MSTFHGLVLVRKPTGVTSHDVVARLRRILGTKSVGHAGTLDPLA
                     EGLMVALVNEATKLSQYILEGDKGYHLKARLGVETDTLDITGQTLKTSPVTLAADQVR
                     EMGLQLQGSMNLKVPIYSAMKINGQKLYDYARKEQEVQVPHKDMNFWDMEFLGFDGTQ
                     AEFSFRCSKGTYVRSWIELLGSKLGCGATMSALTRTWSDPYKLEQSIWLEDLEADFKA
                     GRPIAAMIPMEMALPFVKKVRVKGHDQTLLGNGQISHELRSFLITLFDPQKDNIIQVL
                     SLTTGKLLALVGIEKDRGFVIKRVIRY"
     gene            18880..19149
                     /gene="rpsO"
                     /locus_tag="VIG33_18060"
     CDS             18880..19149
                     /gene="rpsO"
                     /locus_tag="VIG33_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY8272803.1"
                     /translation="MAVTKEVKAEIVKKFKRSDLDTGSSEVQIALLTSKINELTGHFA
                     IHKKDHHGRRGLVTAVNQRRKLLDYIRRIDVKKYEVLLKQLDIRK"
     gene            19304..21397
                     /gene="pnp"
                     /locus_tag="VIG33_18065"
     CDS             19304..21397
                     /gene="pnp"
                     /locus_tag="VIG33_18065"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY8272804.1"
                     /translation="MKTTVTTSVGGKQITIETGRLAKQADGSVLVTCGNNMVLVTAVS
                     SKKASTLDFFPLTVEYIEKFYATGKIPGGYFKREGRPTNDAILTARLIDRPIRPLFPE
                     GYRYETQVVATVLSADGAFPIEILSSLGASAALHLSDIPFSGPTSAVQVGRVDGEFIA
                     NPTPQQMEKSDMNIIVAGTRNGLLMVEGETKFITEADALAALKFGHQAMLPLLNAQDE
                     LREKTGSKAKRAFTAAQIDPEFRMKAEELLKDQISKSLTIKEKQERYAAGDAALAEAK
                     ETLLSTITDKELLSVRSKELSSIVEDLKYKSARSMILDKAVRIDGRDMKTVRPITCEV
                     ALLPRAHGSALFTRGETQALGTVTLGTGDDEQMVDTLLGLQTRQFMLHYNFPPFSVGE
                     VGRFGGQGRREIGHGNLAERALKSILPDHAKFPYTIRIVSEVLESNGSSSMATVCAGN
                     LALLDAGVNIKGNVAGIAMGLIKEGDRVAVLSDILGDEDHLGDMDFKVAGTTTGITAL
                     QMDIKIDSVSFAVMEQALAQAKEGRVFILGEMEKVMKLARGQISEFAPRIETIKIKPD
                     KIREVIGAGGKVIRSITEATGVKIEIQDDGTINIASADPEATKKAITMINEIIAEAEV
                     GKAYKGRVVKIAEFGAFVEILPNTQGLLHISEIANERIRSVTDVLKEGDLIDVKVLEV
                     DRAGRIKLSRKALLN"
BASE COUNT         5972 a         4442 c         5265 g         5804 t
ORIGIN      
        1 taatccgtaa aataagacat agacagttgt agattccgta taaaatagct tcagagaaaa
       61 ggaaaagggg gatttttagt atgaagcggc agtttatacg ttctcatgtc catattaaca
      121 ttcatccttt ggaacgctgg attcgcatta ttgctggagc gatcttggtt gtactggctt
      181 ttcctggcgg tcagattaat ggctggtact tattaggagt gattccgctc ttaactggca
      241 ttattggttt ctgcccgctt tattatgcct ttggcataaa tacgaatcgc tgggcgcggg
      301 ttaaagttga ccatacgaaa aaataaaggc gatgttaatt aattataata ggcgctatta
      361 tcgcagttct aactgggaga aggacaataa tgttgtcaat tgagaacatt ttgtgtttct
      421 gaactggctg actttaaaac taatcaggag tctgaatgaa attattttct cctcttccca
      481 gccctgttga agtagaaaga atttttttat tcctcagctc gcggcatcta gataaataga
      541 tcatggtgca ggctctgcag attctgtaaa gactctcctc gggcctgtag ctaattcaaa
      601 aactcaaaac ctccttgcac aactgtctac ttttggatac agcctcggtt ctgctcatgg
      661 ttggaaatac taagttattt cattcactta acttttaagt ggccatggaa cggagcgtgc
      721 tctgtaggcg agtgatgtgt ttaaccactc actccaaaag gaaattaaaa gataagccta
      781 tgaaatactg tattaaaaac tttatcgcga tattatttgt tcacttctta tttatagcgc
      841 cttcctatgg tgaagcggtg gatagccctg agaactggat catagaaaaa atagagtgct
      901 ctggaaatga aatcaccgct tgttcgatta ttgaaaatga gatttatctt cgcgccggtg
      961 acaaggtcaa cgaagagcaa attgaaaatg cccagctgcg cttaaagact ctcggtcttt
     1021 tttatgatgt tcaaatgtcc ttgcaaaaag gagtgatccg cggccaagtc attctggtgg
     1081 ttcaggtgca agaacgttcc gcgcattata tgaatctcag tgggtactct tataaatcga
     1141 atacggcgag tggaatcatt ggcattgggg atcgcaacct gtttggtttg gggaaaaggc
     1201 tggaggtcga tgttgaaaga tcatttgatc agaatcgcca gagtttcaac gccatgtttg
     1261 aagatcctaa tattttcgga agttcaaaat attttagctc agtccattta ctctacttca
     1321 aagttgataa tgatgatatc cagtacaaaa gctcgatatt aagcatagcc cttggtcgcc
     1381 gcatttttga tttttcctat atcaaactca tagcccgcgg aggcctttca gaattttcca
     1441 cttcaggagt ctcgaacgga gaatataaaa gcctcggaga gggcgtcgag tatggatgga
     1501 atactctgga tgacctttac tttccaactt ctggtactac tttggctgca ggctcctatt
     1561 tttcagacat aggtgcaagg tattatcttt cctcttcgtt tatcaacagt ctttataagg
     1621 attactattt aactctagaa ggaggattgg atctcgataa attcgattca gactctgctt
     1681 ttaacggaac cctcagcttt cgtcctgcta ttgccaaata cttcaccttt cataggcagg
     1741 gtcaacgcat accggataaa atgaaaatct tcttaggtgc tgatgccttt aaccttcccg
     1801 ctacaagcag ccgcatcgaa ttcaatggca cgctgggcgt ttcttatctg atcgattcat
     1861 tcggaaaaat agatttcacg gtttcattcc aggatatgag atgaaactcg taagtctatt
     1921 tatgatattt cttcctggtt tttgttggag tctggaggct atgttacagc cattacaaaa
     1981 aaatgaagcc gccatccaaa taaaaggagt ggaagttcca cgggagcagt tgaaaaggca
     2041 tctcaaatca ggactgcaca gtcttttttt gtttgagttg gcggccactt taaaatcatc
     2101 ttccaagtca gaactcttgt tcaaagaaaa atatcggtgg aaggtctatt atgatctttg
     2161 ggatgaagtt tatagggtga ctgtgcaagg ggacgacaat actgagtcga agatggaatt
     2221 caaaaccctg gaaaattttt tcaccgcact ggaaaatcct cagtttcgaa atctgcagac
     2281 tctcgtggtg gtaaaaaact ttcctaaaaa tggaactttg ggaattcgca ttctattgaa
     2341 ccctgtttct caagagaccg tggagagggt taagaagtgg gttcatcagc gccgcgtcct
     2401 cggaagctca ggcgcggtaa ttttcggtgc gaaaagtggc gaagccacgc aggaaggcac
     2461 ccccagattt cttagcctct ttaacgaaat tctgggttat gagatgaacg atgcgaagca
     2521 agactcggcc tgggcttttg ccggagtggc accgctgctt attcaggaga ccaagcaatg
     2581 aaacgacacc gtgtgcgtct ctggttgatt ctttcgctga tgacaatggt cgccattcct
     2641 ttagcgggag cttattttca gattcaagac gttttgaatt ccagtttctc catgggctcc
     2701 gaggcagaga gcattgaagc tcttgagatg gggcagaccg ctctcaaaga gttggctaaa
     2761 aaatctccgg catcagaaca aaaatatcac gagcaattct tgaaaattca agaactcaag
     2821 ctgagccttg ctgagcacgg tctgatcaga cagggattgg taaattccta caccttttat
     2881 tttctcgtga gttttattct gatgatgctg atctcgattg gttttgccgt gttcttaagt
     2941 cgaaaagtgg caagagctta tgatcaaagt ttcgaggaac tggtgcgcca aagagaccgc
     3001 agtcaatttt tagagggaat ggcgcgctgg caagatgtcg ccaagcaatt ggcgcacgaa
     3061 attaaaaacc ccctgcagcc cattgggact tggatgagaa atctggtagc tgttttccca
     3121 aatcaaaaaa agccagagga tttttcaatg ttattggatg aggcggcaga aagcattcat
     3181 caggagattc atcacctgca gagtctagta gatgcgttca cccaattttc ccgtctgccg
     3241 gaggctcaat ttaacaaagt tgacctcggt ttatttgttc gggagtttca gcaacgctat
     3301 caggagtctt gggatgtgga ttggcagttg gcagtcacct caaaagaagc tcttattcag
     3361 gccgatgtga agcttttacg gcaagtcctg gccaatctga taaccaatgc ggtggaggcc
     3421 aattgcaagg atcggattgt tgtccacatt caagtagccc agaaaaatgg ccatgtggag
     3481 ttggtggtag aaaacagcgg aaaagtgatt gaggccgctt tgaaggaaaa aatatttgag
     3541 ccttattttt ccaccaaatc cgcagcagca aatttcggcc tgggtttagc cattgtcaga
     3601 aaaatcgtgt tcgatcatgg cggcgatatc aacgtcgaag attgttttac gggggccagg
     3661 tttaagctga catttccttt gataggagaa gggcatggat aattattgcc ggattttatt
     3721 gattgatgac gacaaaaaca ttcggcgcag tcttcaagtg gctttgcaaa acaaaggatt
     3781 tgaagttcat ttggcgcaag atgcacaaca agctttccaa acgttgcaga aagagagctt
     3841 tgatattttg gtcgttgacg tcgtcttggg agaattctcg ggcgtcgatt ttttctcacg
     3901 aatccgccaa cagtatccgg aaacgccggt tattttcatg tcaggagaag cgacattgac
     3961 agaggtcgcc aaactgatgc ggcttggtgc cgccgacttt ttagagaagc ctttcactga
     4021 ggaaagactg attttaagta tcgagcgaag tttagagttc tcgcgtcttc aaaaccgcat
     4081 tcaatatctg gaaaagggac agactccgaa agaagatgag atctatggag attcaaatcc
     4141 catccgcgaa gtgcgagagt tgatttctcg ggtggctcgt agtaacgcgc atattctcgt
     4201 gactggtgaa agtggcactg gaaaagagct gatcgcaaag cagattcatc tgcaaagcaa
     4261 aagatcgaat gaggccttca tcaaaataaa ttgttccgcg attccggaaa ctcttatcga
     4321 gagtgagctt tttggtcatg aaaggggctc ttttacgggg gcgacccagt ctaagaaggg
     4381 ttatttcgag ttggcgaata agggcacttt atttttagat gagatagggg acatgtcgct
     4441 tgctgcccag gcaaaaatct tacgggccat tcaaagtggt gagatccagg cagtgggttc
     4501 aatcagcgtc cgcaaggtag atattcgatt aatagccgcg actcacaaag atttgaaaaa
     4561 ggcggtacag acgggtgagt ttcgggagga tctttatttt cgaatcaacg tcgtgccgat
     4621 tcatctttgt cccttgcgcg agcgaagcga ggatattcct ttgctagccg agaaactcat
     4681 tcgcgacatt gctctggaga atagttatat tgaacgaact ttttctgcgg atgccttaga
     4741 gcttatttca cgttggtcct ggccgggaaa tatccgtgag cttcgcaatt ttctagaacg
     4801 cctcttaatt atggggggag caggtttgat cctcccggaa gacctaacaa tgcccttctt
     4861 ttttaaagaa actcaggagt cccttcatcc ggtttctctc aaagagttca gagactctgc
     4921 cgaacgcgaa tacatcgtgg gagttttaaa gaaggtgggg gggaatatca gcaaaactgc
     4981 agaactcctt gaaatcgaac gcacgtattt acaccggcgt ctcgggcact ttaaaattga
     5041 aaagagagag tacttataag ttttgataat ttatctgcga taggaccttc accgatcgtc
     5101 caacaagttg acaggtctat tccaaaatat ttttttaacg gttataagtg ccgtctacaa
     5161 aatttttgac ctaggaaagg ccttgccaat gaaactaaaa acaattttga ctgctatgat
     5221 cttattaaca actcactttg gctgggcagc agtaaaggat gacgagtcga cagataccct
     5281 ctctatcgaa gttccaaaac atcaagtcaa agtcggcctt agtgctgagc ctatctggtt
     5341 attggttggt ggcgtcggag ctaaattcga atactttgta acagatcata tttcagttgg
     5401 aatcaacgga gtttacattc cagaacatca aattgagacc tcaagttcaa aagaccaaag
     5461 cctcaccagt gagtcttacc gttggtccat gaatgaaatt aatttaggca gcaatatcat
     5521 gttaaccgga agcctctcca ctaacggagt ttatctcaat ccggctgttg gttatcaaaa
     5581 cactcgcatt tcagattacg gagtattaaa attaaaagga tccttgtcgt caccacaagc
     5641 tcgcttgacc gctgggtatc aatttgtcaa tccagcgaat aacttgcgat ttgcactcgg
     5701 cgctggtttg cgcatgattc aagatagcga tattcttgtg aaggatgaca ccggcgaaga
     5761 agtatatcgc caaaaaactt caggccttgg cggattggct ttagactgtc acttaggtta
     5821 cattttctag gtagttgcga taaggaactc ttcaccacta gagggctgca acccgtcggt
     5881 tcgccctgag aagtaacgtt ggatgatacc gtctctggat atgtgctggg tatttttgaa
     5941 gccggtttcg cgggccaacg ccattatctc tgacggctca aagaagctga taaacggcgt
     6001 tcccgcagcc cgtgcccgct catacaccat ctgcagttga gtgcgttctg ctgggtcgat
     6061 aagttgcagt ggcagtatga atgtcatagc caggatggaa cccggacaga gtgaagcaat
     6121 ctggcgtaac gtcatcgagt tcgcctcctt agtgaggtac atggagacac cagtggaggc
     6181 gataaccgcg ggttggctcg catcaaaacc gtttgctatc agcttttctt tccacgactc
     6241 tcccgcttcg aaattgacgg gcacaaagtg caaattttct ggagtatcga aaccaagctc
     6301 gatcaaccga cgccgtttcc actcctgagt gccgggctgg tccacttcaa aaatttgcaa
     6361 ctgagatgcg atctcaggtc tgcgttgggc aaaggtatct agtcctgctc cgagaatgac
     6421 atattgatgg atgccgtggc ttaatttttc caccaccaga tcttcaataa gtcgagcccg
     6481 agccacgatt ccggcacggt agccccttgt accctgggga tgcatatcct gacgctgccg
     6541 ccagcctgcg tccgggttta ccaattgcag gccaatctcg tcttcgagca catgcgggag
     6601 gggatcgatc tgaacgtgca tggctcgcca cagagcgacc cgcacggctg aactatctgg
     6661 tgcgctggtt tgcttattgt ccacatccac gatctgtctc cctatcaaaa aataaattac
     6721 gccaatgcca ggttagcacc aaaaatttat gatgtgaagt attcggaatc gcgccctaac
     6781 ttacgtactt acataacgaa tagcgcccag aggcatcatt ctggactagt aatttaatat
     6841 ttggactgaa ccgccaaagg cggttcagtc caaagcagga atcttaatcg tatcaacttc
     6901 ccaaccactt tacttttcat ctttagcgat tttttcctgg gcttctttat cggcatgacg
     6961 gctgggcttg cctttcagaa tccattcaaa accgactgcg ataatggcgc cgataaaggt
     7021 tgcaacgaca tagatccaat cgccaatgcg ccattgttgc ctacattgcc gggccctgac
     7081 gctgcgccta gaattgtatt tatcaatccg gtcgtcaaca ctgtttctat cataagagct
     7141 ttccagcttt caaagccggg aatgggaact gtggctccaa gatgtccagt gtttccaaac
     7201 agggctctta gaaaaaagga ggctagaagg ccgccgccta tttgcgcgaa taggtacaac
     7261 ggccgggttt atatgggcac cactaacatc tcccatgaaa tatataatag ctctaaaatg
     7321 gcgtttaaac ttcctatcct ctttctgaag ggataacgtg caggaaaggg ttttatgatg
     7381 ggcatatcca tgatttagaa atgacagcaa aaattgaaaa atatgaaagt aaaagaaaac
     7441 actaagtctt caaatttgac ttgggtaggg tccgccccta ggaaaaatgc ttttatctgc
     7501 cttagtcaca gaatttcaca aaagagtcaa atattcaagg cacagtaagg aaccccgcaa
     7561 acacgaatca tttttaaatt ctaacctcgg cccattttac ttaaaaagta attgtgctac
     7621 tgtatcaggt agtataaaaa ccgaattgaa ttaaaaaata caactcaaaa cggaggtctt
     7681 attgcatgga ctcactcgag agatggtcat ttatgaaggt ctttgtttta agttacattt
     7741 taatgctcgc gcccttagtt gggcgtgctg atctcttcgt agatgaccac gttacccgca
     7801 ccgtgatgac ggaaaatgaa cggcacctac cggtcgttca ggttgcgaaa agctccggca
     7861 ttctcctggg cgagtctctt ctcttgactg cgcagcatac gaccaagaat tggcagagtt
     7921 attcctacca gggggccaca atgttttatg ggccccataa cgaccattac tacttcaagt
     7981 tcgaatccat cattgaggaa gacgcagccc tcgattatgc gatcgtgcga atctcgtgga
     8041 agaatggagt catcccgcct ggtctacaat gggtgaaatc catcgttatg cctggtgaga
     8101 tcaaacttgg cgtaattcat actatggata tctttacctt gggttctcct tgggacaaga
     8161 tcccaaatcc aatgaaatcc agcgggagca aaatcgtgat tcttcccgac agtcaaatta
     8221 agtatccgct acttttcaat gctggagtga ttgaaggaaa ttccggtggc ccgatcttca
     8281 cggcagatgg gaagctcctt gggatcgtca cgggcggtcc tcacaaattg ggagaacccg
     8341 gttgggaagg taacgacccg aataaaatag aatcctggaa ttggggcact ccaatccaaa
     8401 acattttcaa aattagcaaa gtgctccaag agcttttccc cagaggagaa taactctagc
     8461 cagagccttt ggcggcagca aaacgttgta atataaaatc tctacacgat acattccctg
     8521 ggttccttcc gggatcgaac cccgcaggag atccgctggt tccgctggct tgaggcccgc
     8581 ggaaattttg aaaccacagg aatcgaaccg tgacactttc agtgtcccgt tcttcttcct
     8641 tggaatgttt ctgtaagatt gtatcgacat cgtagaacat aatggtggcc ccggggagaa
     8701 tcgaactccc acgcccttgc gggcactgga ttttgagtcc agcgcgtcta ccaattccac
     8761 cacagggcca tttaatagaa gagctatctt gtaattgaaa agagccgctt cattcaagct
     8821 gtttttagca gttcttaaac aagggagcgg cgagttatta ggggtgccta tctagaaagc
     8881 ataaattaag cgcgcattgg ttgaggtcat acgttcttcg gtaagggctg tttgggactc
     8941 gatcccttgg ttaatccagc tataaagaat ttcgaaatta aagttctttt gcaggacaat
     9001 ccctccgcca gcttgataac caatatgcgg agagtgttgg gcgttgtcgt cgcctgcaac
     9061 aatggaagtg tttacgccca ccgccgcata ggcatactta ggagccgcca cgataaaatt
     9121 aaccgcgggc actacattcg taaggtcgga cgtgccctca ggtcgctttt ccagttttaa
     9181 aatacttaag ttgagatcga atcccatgcc tccgcgggga atttgcttat atcccatttt
     9241 aaagccgagc tgtttgggaa ttttggaggc atcttcttta agaccttttt cgaggttccc
     9301 agtgagctta tttttaatat cactttgcaa actattgacg gacaggtatt caaaccccag
     9361 attaaaacca tccaccatat agggcgaggg accaacgagg gtatcagggt cgtagttata
     9421 agtgggtgga gccgtcttgc gaagtttggt agtggcagtc ccctgggcca ggctcattga
     9481 agtgagtgtt aaggatatca tcgtcatcaa agttattttt ttcatctgaa tctcccttta
     9541 aatcctttta aggtgtttgc ctttttataa ccaagagtcg ggccaattta gatggaatag
     9601 cttccaatag aatgtcctaa tttgagacga agccggtttt tgttaaacag aactcatgca
     9661 gctaggcggc taccccagga taagatcatg aaatcctttc aaggtgtgtc gcaaaactgg
     9721 tcaaattttg aagttttgga gtcaaaatgc ccccctcttt caaggccccg ttcgggtata
     9781 gatatcaaag gcttagacgc attatatcaa aaaagtcact catggcatat tcgttgctct
     9841 aggttcttgc gaatcagaat gctaattccc gaggagaaca ctatgaacaa atatttctat
     9901 atccttgcgg ctctcagtat cgtaccgaat ttgagtttgg ctcaggcggg aaattttgat
     9961 gctgaagtcg atcaagaact tgatcaaatg tatgccaata agcaggcagg aactgtggcc
    10021 tcacccgcgc ctacaagagg atctgttggc ggagttgcgg ctcaagggag tcagccgatt
    10081 tacatcctca atcaggcaac gcccactgcg gcgaccaaca ccaatgtgca aactcaaacg
    10141 gctcaggtgc aacgacagcc tgtgactttg attgaatcta cgccgttgac agaatctaaa
    10201 gccgagctaa tgagaaaatc acgacaagac gctgaagttc agacggagca gaagatcgtc
    10261 gagaagctcg agtattctcg ccttgaggat gaaaagaagc gtgcggaagt tctcttcggt
    10321 aataaatttg aacaaatgca gggaaatacc acacaaatta aagccgagaa ctcaaatgtg
    10381 agcgtcaata ctcaacaaca ggcaacgcaa gtccccgttg ttgtgcaggc cccagtaacc
    10441 cccattgaac ccaaagaaaa tttccgtgat gtcgttcgtg aagagcttca caacgccatg
    10501 aaggtggaag aacccatcga agtggcggcc gtagagacca agtatttcgc ggccatcgct
    10561 ggtattggcg actatcccga tgttagaaat gttcgcggaa attatgcctt cggtgcggcc
    10621 tttggcacta aatatgatca actcatcgtt gagggatcat ttttattgtc caattacact
    10681 gttgagaaaa ttgattgcaa ttgtgtaagt tattatccga ctttggtgga taccaatcag
    10741 tatcaagggg ctgtcgccgc taaataccaa atgatgtcgg gacttgttcg ccctgtggtg
    10801 ggtggtttgg ctgcttatag ttatcgtaag tacaattgga caaatgtggt gaacgcttat
    10861 ccgtatagca attacggaac cagcagtgac tcccatgcgg tggatttggg tgtaactgct
    10921 ggagtggatt tagagttaag taagaagttc gcgctcggct ttgaatacaa atacatgttc
    10981 aatctttcca gccgggttaa taatcagtct atcgtcactg gacctcagta tgggactccc
    11041 atcgaaaaac tgagctatta cacgatggct ctcgcggcta aagtgaattt ctagtcatta
    11101 ttttttattc tcctcttcaa ggagtgcctg ggagctctta tgagaaatca taagagcttt
    11161 cttttttttt acgctagtgt cattctagct ttatgaagat gtttcttgtt ttaaccttga
    11221 gtgttttgtc tggtattttt tctttggctg ccactcctgc ggtgatttct caatccgtag
    11281 ggcgggtggg cgagcgaatt gtcacgagtc gtgaggttca gatttcggcc gttttaactc
    11341 gcgcgctttt tccaatcaaa gaggcaaaac caagtgtaaa cctctatgaa gtgcatctgg
    11401 gagattccgc ttttatggga gaggtgaccg ccttgttatt agaggtggtg gtggacaaag
    11461 aggccgagag cttctctgtg gctaaagttt ctgaggaaga attgcagcag tccttgcgga
    11521 aaattgaaaa agtcgtagag ggcaagactt attgggagtc cttggaggtg agtcccgccg
    11581 agctgaaaag atttgtcctg caaaaattga ctgcgaaagc tttcattcag tttaaaacaa
    11641 actcaatggt gggagtggtc agtgatgcgg aagctttggc ttattatgaa aaaaaccgtt
    11701 tgaagtttgg cgatttgccg ttttcctctt ttaaagaaaa tatcaaaacc tttttagctc
    11761 agcagcagtt ggaagagcgt agccggacct ggtttgaaat tattaagcgt aaatacaaag
    11821 ttcgtaatat cttggccgag aatatgaaga ataaggtggg tccaaagtga gctttaagat
    11881 gcgcccctta ttagccgatg atgaagccca ggtattacag cttgttgccg atctttaccg
    11941 ccttcatccc tatctggaag gttttcagtg gcccagcgaa actttaagag cggagttcca
    12001 aagtaccgat ggctgggggg tttttaaggg cccgcgttta gtaagctttg tactttatag
    12061 agtgacgccg gtggcttggg agatttcagt cgttgccagt caccctgaat tttggaaaca
    12121 gagtttaacg cgtagcctcc ttggacattt gatcatcatt aaacctctcg atcgagaact
    12181 atggctggaa gttcattgtg aaaatacgag ggcttggcga ctttatgagt ccctgggctt
    12241 tgtgagaact gggctgcgag agaattatta ccctaaccag ggtacagctt ggctctatac
    12301 tctgagataa acccttgcat cgcttagcct tttttgctag ggtcagccat gaatgggcct
    12361 tgaagggccc attttttatt tccggccagc gcccgacgct tcaggaattt gacgttagga
    12421 atttaataaa taatgaccga atcaaaccca tcttggatgg aaaaagtaga aaaaatggcc
    12481 gaagaagcaa cttccggcga aggctgtttt ctttacgaca ttgagtttat tggtgtgggt
    12541 aataaaggcc ggactctccg tttatacatc gataaagaca ctccgcaagc agttcctgat
    12601 tcagaagaag gtcagggtgt gagtattacc gattgctcca acgtatcgaa ggctttgaac
    12661 cttttgttgg atgttgaaga tattatcccg ggtgagagtt ataatctcga agtttcaagt
    12721 cctggcttgg agcgcattct tcgtaagccc tggcattttg aaaaagctgt gggcaaaaaa
    12781 atctgggtga aaaccagtaa atcctttgaa tcgtttggcg tcactgacaa gaaactcaaa
    12841 gcggcaaaac aaattgatga agttttaaag gcttttgatg gcgagactct gagcttccaa
    12901 gttaaagacg tggaagtaaa tatccctctc tctgctgtag aaaaatcaaa agtgctattt
    12961 gtattaacta agggacaaaa aaaataactt agaagaggtg caggaatggc tgacaacatg
    13021 tttacggatc tcagtcgcgt tattgaacaa gttggcaaag acaaaggtat cgacaaacaa
    13081 gtcgtagtgg atgctattac tcaagggatg cttgttgcag ctcgcaaaaa atacgggact
    13141 tatcgtgaga tcgaagccac atataacgaa gaaacgggtg aagtagagct ccatcagttt
    13201 aaagaagttg tgcctcgtga aaaatatatc gatgaagaag tggaaatccc tatggatgaa
    13261 gctttgaaat tagacccaca agcccaagaa ggggactcca tcggtatcaa aatggatgcc
    13321 ggtgagttgg ggcgcattgc cgctcaaacg gctaaacaga tcattatgca aaaggtgcgt
    13381 gatgcagaac gttccatcat ctttaatgaa tttgaagaga gaaaaggtga aattgcttca
    13441 gggatcgctc gtcgcgtaga aaaaggtgcg attgttgtgg atcttgggcg tacagaggca
    13501 tatattcccc ctcgtgagca aatccctggt gagtcttata aacccggtga tcgtattcag
    13561 ggttatttat cggaagttcg gcaaaccacc cgtggtccac aaattatcat gtcccgtgcg
    13621 gatgagcgtt atctaatgaa gctttttgaa atggaagttc cagaaattta tgacggaatt
    13681 gttgagatta tgagtgcggc tcgtgaacct gggcaacgcg ctaagatcgc cgttcgttct
    13741 aaagacaact cagtggatcc agtgggcgct tgcgtaggta tgaagggctc tcgtgtacag
    13801 aacatcgttc aagaacttcg tggtgagaaa attgatatta ttccttggga cgaagatgtc
    13861 acccgttttg cttgcaacgc cttggctcca gctgaaattt ctcgggtttt cgttgatgaa
    13921 gcgaacaaag aaatggaagt ggtggttccg gactctcaat tgagtttggc tatcggtaaa
    13981 cggggccaga atgtgcgttt ggcagcgaaa ttgacgactt ggaaactaga tattatttcg
    14041 gaatcctcgg cctcttctcg taccgcagaa gctattttca acttgatgtt gcttccaggt
    14101 atgagccaaa cgatggcaca aaatatcttc caatcgggtt tcggttcttt ccaatcagtt
    14161 gcaaatgctt ctgttgaaga gattatgaca attcctggtt acgatgatcc agagcgtgct
    14221 gagaagttga tgggggaagc taaaaacttg atcttgaagt atgaaaaaga aggcttgcct
    14281 attcccacag ctccttcggc tgtaaaggaa agcaaagctt ctggttctgc taaagaacaa
    14341 gcagaccttc gtttgaaaca agagttacaa aaactcgatg ctcaagctga agataaggag
    14401 taatcttcgg cctttatggg ctgaagttgg actgagtttt aagtagtagt taaattattg
    14461 taggagaatg cgtgagtaat ccaaaagttt ttgaatttgc caaagaaatt gggcttacac
    14521 ccttggcctt gatggataag atccgcgagt ggcaaattcc tgtgaagagc catatgtctg
    14581 agctagagcc tgaggttctt gagcaactta aagtaaaact cagtggcgga aataaatcca
    14641 ctgaggaggc gaaatccaaa agaacgacga ctcgcaaagc tcctgcaaaa aaagtagcag
    14701 ccccagcggc tgaagccgct gccgcaacag cgaatgtcac tgctaaatcc acagtcgttc
    14761 gtcgaaaagc tacaaaagag gccgaggacg cggaaattac agctcagtta aaatcggcgg
    14821 ccgataaagc agaagctgaa gagacggcaa aagctgtggc aaaggctgtt cctaaaaaag
    14881 cggcccctgt agttgaaaaa gaggtcccag cgccaaaagt tgtagttgaa atgcctccgg
    14941 cgccagttgt agtggctcct ccggtgatag aagctccagt gagggaagcg gctccagtcg
    15001 tagaagtgcc tgtagctcct tcgtcggcac cgactccgcc agttgtggct ccaccgactc
    15061 ctgctgcagc ggtatctgca gccacatctg tgggtgtggc tcgtaaaaag gaagtaacag
    15121 ttggcagtag tggggtggcg agtgccgcaa ccgcagctcc cagtattggt cgaagaaata
    15181 ttatcggtcg tatggatctt tctcgggtgg ctcagcaggc tcctcaaaga tccgctgagc
    15241 gttcgaccag cgccggcgat cgccaaggtg gctttaatag tggagcccct cggacaggtg
    15301 gctttagtgg ccccgcacgc acctccactc aaaatcgtaa tatccgtcca ggatttgtgg
    15361 ctcaagctca accagacgtt ccagttgagc ctattgagag ccgtcatgga aaatttgatg
    15421 ataagagaaa acctaaattt tctccagttc cggttgtagc ggccggtccg tctgcaaaag
    15481 agcgtgaaca agaagagcaa atctccaatt ttaatccggt tgagtttcgt aaacgcgaga
    15541 tggttttcca gcctaaaaag aaaaaaggca tgttggaacg tgcgggtatg aagactcaga
    15601 tcacgactcc aaaggctttg aaacgcatta ttaaagtgaa taatgccatg aaactgagcg
    15661 atgtggcgat ggaaatgggc atcaaagcgc ctcagattat caaagttctt atgcaaaatg
    15721 gtgtgatggc aaacatgaac actctgcttg attttgatac catcgctttg attgttccag
    15781 agtttggttg ggaagcgcaa aacgtcttta aaacagctga tgatatggcg acagaaactg
    15841 ctttcggaaa tttggatgca gcgatgattt cacgtcctcc agtcgtgact attatgggtc
    15901 acgtcgatca tggtaaaacc agcttgctag atgctattcg taaagcgaat gtggcagcgg
    15961 gcgaggccgg tggaatcact caacacatcg gagcttacag tgtgactttg gatgatggct
    16021 ctttagtgac gttcttagat actccgggcc atgaggcttt cacgaaaatg cgtgctcgtg
    16081 gtgcgaatgc cactgatatt gcggtgatcg tggtggcggc agatgacgga atgatgccgc
    16141 aaactgcgga ggctattaat catgccaaag cagcaggcgt gccgattatc gtggcgctca
    16201 ataagatcga taagccagga gcaaatcctg agcgtattaa gcagcaatta actgagcttc
    16261 aaattgttcc agaagaatgg ggcggtaaca ccatcttcgt tgaagtttca gcattgaaga
    16321 aaacgggtat caaggagctt cttgaaaaca ttcacttggt tgctgagctt caagatttga
    16381 aagcaaatcc tgagcgttca ggaacaggtc tggtgattga agctaaaatg gaaaaaggca
    16441 aaggcaatgt tgccactttg ttggtgaaag atggaaccgt taaagtagga cagcacattg
    16501 tggctggaac tattaaagga cgtgtccgtt ctttgactaa tgataaaggg gaacgtgttg
    16561 attctgtagg acctggcatt ccggtggaag ttttggggct ggaaggcact ccctcagcag
    16621 gcgatcgttt tgacatcgta aaagatgaag aaacagctgt tgaggtttct gaaatgcgtg
    16681 cagaacaggc gaaaaaagct gaagtcgtgc cagcttcaaa aatgtctttg gaagatatct
    16741 tcgctaaagc gactcgtggc gacgtgaaag atcttccaat cattttgaaa gcggacgttc
    16801 atggttcttt ggaagctgta agtgggatga ttcagaaatt agccacagcg gaagtaaaaa
    16861 ccaaaattat tcactcagca gtgggtggta ttagtgaatc tgatgtgttg ttggcaaata
    16921 cagcgaatgg catcattatc ggcttcaatg tccgtcctga cggcggcgct caaacagcgg
    16981 ccaagcgctc aggtgttgat atcagaacct actccattgt ttatgagttg gtggatgatc
    17041 ttaagaaagc aatgtcaggt cttcttaccc cggatgtggt ggaaaaagtt atgggtcgcg
    17101 ctgaagttcg taataccttc accgtgccta aaatcggaac cattggcggt tgcttcgtta
    17161 cagatggcaa aatccaaaga tctaatatgg cccgtttgat ccgtgacggt aagatcgttt
    17221 atgaaggaaa aatttcttct ctgaaacgct tcaaagatga tgcgaaagaa gtcgccagtg
    17281 gctttgagtg cggtatcggt attgagaact tcaatgatct taaagtgggt gatgcgatcg
    17341 aagcctttat taaagaagaa gtggcccgtg agttgactta tgaggcaaat ccataatgaa
    17401 aaaagaaggc gatggcgggc gtagaattgc ccgcgtcgag cgagaagttc agcagacaat
    17461 cgcccgtttt ttaatttcag gttttaagtt gccattgcct ggcattgtga ctgtcgctcg
    17521 ggtgcaaatg ccaggggatt tgcgcactgc taaggtttat atcagtgtta ttggagaacc
    17581 ttctcaattg gatgaagtgg taaagcttct tcaagggcgc gcctttgaaa ttcaaaacta
    17641 tctgggcaaa gagcttaaga tgcgttattg tccaaagttg tctttttatg ccgactttga
    17701 cactcaacaa atcttaaagg ttgagagaat cttgcaagat ctggaagcag aacgaaatca
    17761 atctaaaaaa gttaaagacg actctagcga agaagactca tgagcacatt tcatggactg
    17821 gtccttgtaa gaaagcccac aggagtgacg agtcatgatg tggtggcaag gcttcgccga
    17881 atcctaggaa ctaaatccgt aggtcatgct ggaactttgg atccactggc tgaaggcttg
    17941 atggtcgctt tggtgaatga agccacaaag ttgagtcagt atattcttga aggcgataag
    18001 ggctatcatt tgaaagcccg attgggagtt gaaacagaca ccctcgatat taccgggcaa
    18061 actttgaaga caagtccggt gaccttggca gcggatcagg tgcgtgaaat gggtctccag
    18121 cttcaggggt cgatgaatct caaggtgcct atttattccg cgatgaagat caatggtcaa
    18181 aagctctatg attacgctcg caaagagcaa gaggttcagg ttccgcataa ggatatgaat
    18241 ttttgggata tggaatttct aggctttgat gggacccagg cagagttcag ttttcgctgt
    18301 tcgaagggca cttatgttcg ctcttggatt gagcttttgg ggagtaaatt aggctgtgga
    18361 gccacaatga gtgcgttgac gcggacctgg tcggatccat ataaattgga acagtccatt
    18421 tggctggaag atcttgaggc ggactttaaa gcgggcagac cgattgcagc catgattcct
    18481 atggaaatgg ccctgccttt cgtcaaaaaa gtgagggtga aggggcatga tcagactctt
    18541 ttagggaacg gtcagattag ccatgagctg agatcttttc tgatcactct ctttgatcct
    18601 caaaaggata atattattca ggttttatct ttaactacgg gaaaattatt ggcgcttgta
    18661 ggtattgaaa aagaccgagg ttttgtcatt aaaagagtga taaggtattg accctttttt
    18721 aacttcatgg cacaacctct actcaataaa acacagacta gccgggcgac agccatgacg
    18781 ctcccacgct ggttacgggt tctgaagggc ctgtttaaca gagaaaaagc ttcagaaaag
    18841 ctttaggaaa agcgagcttt ctcaaaaaag gagactttaa tggcagtcac aaaagaagta
    18901 aaagcagaaa tcgttaaaaa attcaaacgt tcagatttgg acacaggttc ttctgaagtt
    18961 caaatcgcac ttttaacctc taaaattaat gaattaactg gtcatttcgc aattcataaa
    19021 aaagaccatc acggtcgtcg tggattggtt accgctgtta accaaagaag aaaacttctc
    19081 gactatattc gtcgcattga tgttaaaaag tacgaagtcc tgcttaaaca attagatatc
    19141 cgtaagtaag caataaaaag gctcggggac cccgggcctt tgttgctttt tggtcaaaaa
    19201 tctctaaaat ttcactcaag cacatactta aaaacatttg gccaccaagg cccgttccac
    19261 caaaaagaaa aaggtgggat attactatac taaaggagaa ttaatgaaaa cgactgttac
    19321 tacttcggtg ggcggaaaac agattaccat cgaaacaggc cgtttggcga aacaggctga
    19381 tggatcagtt cttgttacct gcggtaacaa catggtcctg gttacggctg tgtctagcaa
    19441 aaaagcttct actctcgatt tcttcccttt gacggttgaa tacatcgaaa aattctatgc
    19501 cactggtaaa atcccaggag gttattttaa acgtgaagga agacctacca acgatgcgat
    19561 tttgacagct cgattgattg atcgtccgat ccgtcctttg ttcccggaag gttatagata
    19621 cgaaacgcaa gtggtggcga cggtcctttc cgctgacggc gctttcccta ttgaaatctt
    19681 gtcgagcttg ggtgcttctg cagctttgca tttatctgat atcccatttt ctggtcctac
    19741 ctcggcggtt caagtgggcc gtgtggatgg tgagttcatc gcaaacccaa ccccccagca
    19801 aatggaaaaa tctgatatga acattatcgt tgcgggaact cgcaatggtt tgttgatggt
    19861 tgaaggtgaa actaaattca tcacggaagc agatgctttg gccgctttga agtttgggca
    19921 ccaagccatg ttgcccctct tgaatgctca agatgaattg cgtgaaaaaa caggatcaaa
    19981 agcaaaacgt gctttcactg ccgcgcaaat agatcctgaa ttccgtatga aagcggaaga
    20041 gctgttgaaa gatcaaatct ctaaatcttt gacgattaaa gagaaacaag aacgctatgc
    20101 cgccggtgat gcggctcttg ccgaagcaaa ggaaactttg ctctcaacta tcacagataa
    20161 agaacttctg agtgttcgta gcaaagaact ttcttcgatc gttgaagatc ttaaatataa
    20221 atcagctcgt agcatgatct tggacaaagc ggttcgtatc gatggccgtg atatgaagac
    20281 agttcgtcct attacctgtg aagtggcttt attgccacgg gctcacggtt cagctctttt
    20341 cactcgtgga gagacccagg ctttgggtac agtgactttg ggcacgggag atgatgagca
    20401 aatggtggac actttgttag gtctgcaaac tcgtcaattc atgcttcatt acaatttccc
    20461 tcctttctca gtgggagagg tcggtcgttt cggcggccag ggtcgtcgtg aaatcggtca
    20521 cggaaatttg gcagagcggg ctttgaaatc aattcttcct gatcacgcta aattccctta
    20581 caccatccgt atcgtatctg aagttttgga atccaatggt tccagttcta tggcgacagt
    20641 gtgtgcaggc aacttggcgc ttttggatgc cggggttaat atcaagggca atgtggcggg
    20701 gatcgccatg gggttgatca aagaagggga tcgcgttgcg gtcttatctg atattttggg
    20761 tgatgaagat cacttgggag atatggactt taaagtggcg ggaaccacta caggtatcac
    20821 cgctctgcaa atggatatca aaattgactc tgtttcattt gcggttatgg aacaagcttt
    20881 agcgcaggct aaagaaggcc gtgttttcat tttaggtgaa atggaaaaag tgatgaagtt
    20941 ggctcgtgga caaatttctg aatttgctcc tcgtattgag accatcaaaa tcaagccgga
    21001 taaaatccgt gaagttatcg gagcgggcgg taaggttatt cgtagtatta cagaagcgac
    21061 cggcgtgaaa attgaaatcc aagacgatgg aacgatcaat attgcttccg cggatcctga
    21121 agccactaaa aaagccatca ccatgatcaa tgagatcatc gctgaggctg aagtaggaaa
    21181 agcttataaa ggccgagttg tgaaaattgc ggaatttggc gctttcgttg aaattcttcc
    21241 caacactcaa ggtttattgc acatttctga aattgcgaac gaaagaattc gcagcgtaac
    21301 agacgtgctt aaagagggtg acttgattga cgttaaggtt ctggaagtgg atcgcgcagg
    21361 acgtattaaa ctttctcgca aggctttact caattaatga ttcccttcta taaatctgaa
    21421 ttagacaatg ggattcgagt gctgagtgaa cagcattcga attctcgtgc ggcgtccata
    21481 gga
//
LOCUS       DATBTE010000474         2965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_612183, whole genome shotgun sequence.
ACCESSION   DATBTE010000474 DATBTE010000000
VERSION     DATBTE010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612183"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VIG33_18070"
     CDS             complement(<1..713)
                     /locus_tag="VIG33_18070"
                     /inference="COORDINATES: protein motif:HMM:NF024571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY8272805.1"
                     /translation="MKRQAEDHLKKWYQSKSGRKPLVLRGARQVGKSTLVRNFAASMG
                     LDLIEINFEKHSLKTADKSLDLDLILPEIEYLTKKKFGSKTLLFLDEIQDSPKMITSL
                     RFFKEDRPDIAVIAAGSLLEFALTVEEISIPVGRVQYYHLGPFTFSEFLEALGEQILI
                     NILSDPEHIPSFADQALKEFYLKYLYIGGMPEVIQTYINSGQNLDQVRNVQESILQSF
                     REDFHKYSSRTELSRVRKV"
     gene            complement(858..>2965)
                     /locus_tag="VIG33_18075"
     CDS             complement(858..>2965)
                     /locus_tag="VIG33_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HEY8272806.1"
                     /translation="LIFQVLFLFFAMIINVGLLVHHKVNLQNSVDLAAYYGAMKQAEM
                     MNAIGHVNYQIRQSWKLLSWRYRQIGTAGDYNVHPYDKIVRQIRPDDLEGLPKFGETS
                     TAKDYYDAPAFCITYAPFSPMPTENESTCKHQSTYRTINLFTPPPVIAGFFSINSAIR
                     QASQRALNEAIDRCRYVGQFNFMILAEFMAAYNIDQGDRKQLINLLSRGLSEKEDDFR
                     EINGELASLGIDATLKNNLTEANRSSVNYKIFNSLGTADCNDVGQVNTDPAKWLSEIK
                     VKPAFIYMDFDCESDPSNPRVMPHPKELTSQNLPRYVNELAVDVNYLKQFADPLEAPY
                     NSSVGVEKNPWCMAYVGISAESTPNIPFAPLGGVSLKARSFAKPFGGKIGPWYYSQWP
                     RGSKQSSGGKRIDPLLPWREGDKTATADPRDPTRAANYSRFVGDKAGMKSRAVQGQFG
                     RAIFNLSSAWSGKPPTPSDLDPSIPPDWRPSAEPNFYHWSHLGQTFKAEKSGDILAWD
                     GLQNSPPRMRNLELAAILPDQFDLTYYSIDPDYYHNYYLKLINEYVKVVPGFNYVIRP
                     DLGSRLGHKDLEEFSVREQYKALDYVKTDNPQINEPKVDFENKILYTAKDVYQVLTSW
                     VGKNLSDYSLDPEKFGKCKVSQDKSKNVPTSGDCNNGGRTGYSVKLVSSEYLRANDLE
                     LGGENSGQGAVLNPPPSDF"
BASE COUNT          843 a          692 c          593 g          837 t
ORIGIN      
        1 aagaccttgc gaactctgga aagctcggtc cgacttgaat atttatgaaa gtcctcgcga
       61 aaagactgaa gaatcgactc ttgaacattt cgtacttgat ctaggttttg tccgctattg
      121 atataggttt gaatcacctc aggcattccg ccaatataaa gatatttcaa ataaaactct
      181 tttaaggcct gatccgcaaa agaagggata tgttcaggat ctgaaagaat atttattaaa
      241 atttgttcgc cgagagcctc aagaaattcg gaaaaggtga agggacctaa atggtaatac
      301 tgaactctac ccacgggtat tgatatttct tccacagtca gtgcaaattc cagcaaggat
      361 ccagccgcga taactgcaat atcaggccga tcctccttga aaaagcgaag gctggtgatc
      421 attttcggac tgtcttgaat ctcatctaga aacaaaaggg ttttggaacc aaatttcttt
      481 tttgttaaat attcgatctc aggcaaaatg agatccaaat ccaaactctt gtcggctgtc
      541 tttaaactat gtttttcaaa attaatttca ataagatcaa gccccatcga agctgcaaaa
      601 tttcgaacaa gcgttgattt tcccacttgc ctcgcccctc ttaaaacaag aggttttctt
      661 ccactcttag attggtacca tttcttaaga tgatcttcag cttgacgctt caaagaagtc
      721 ctttatcaga gggttagcac tctgaactat actgttgttt aatatagtgc tgagcctatg
      781 cagtgtcaac gaaatagccc tattttcgtc caacatatgg cccaaaatag ggctattatc
      841 agccataact ccgaaagcta aaaatcactc ggaggcgggt tcaacacagc accttggcct
      901 gaattctctc cacccaattc tagatcgttg gctctcaggt attctgaaga aaccaatttt
      961 actgaatagc ccgtgcgtcc cccattattg cagtcgccac tcgtgggaac attcttgctc
     1021 ttgtcttggc tgactttaca tttcccgaac ttttcgggat caagagaata atccgataaa
     1081 ttttttccga cccaggaagt gagcacttga tacacatcct ttgcggtata aagaatttta
     1141 ttttcaaaat ccactttcgg ctcatttatt tggggattgt cggtttttac ataatccaaa
     1201 gctttgtatt gttcgcggac actgaactct tcaaggtcct tatgtccaag acgggatccc
     1261 aaatctggcc ggatcacata gttaaatcct ggcaccactt tcacatactc attaataagt
     1321 ttaagatagt aattatggta atagtctgga tcaattgaat aataagtcag atcaaattga
     1381 tctggaagaa tcgccgcaag ctccaagttt ctcattcgtg gagggctatt ctgaagacca
     1441 tcccatgcaa gaatatctcc gcttttctca gctttaaagg tttgtcctaa atgactccaa
     1501 tgataaaaat tcggctccgc tgaaggtctc caatcgggag gaatacttgg atccaggtca
     1561 ctgggagtgg gtggttttcc actccacgcg ctgctcaagt taaaaatagc gcgaccaaat
     1621 tgtccctgaa ccgcccgaga cttcatgcct gctttatcgc caacaaagcg actgtaattc
     1681 gcagctctcg tgggatccct aggatctgct gtagccgtct tatcaccttc acgccaaggc
     1741 aaaagaggat ctatgcgctt tcctcctgaa gattgctttg atccccgcgg ccactgcgaa
     1801 taataccagg gaccgatttt cccaccgaaa ggttttgcga aggagcgcgc cttcaaactc
     1861 actcccccca agggggcgaa aggaatattg ggcgtagact ccgcactgat tccaacataa
     1921 gccatgcacc atggattctt ctcaacacct acggaactat tatagggcgc ttccaaagga
     1981 tctgcgaatt gcttaagata atttacatcc acagccaact cattcacata tcgaggaaga
     2041 ttttgcgaag tcagctcttt tggatggggc ataacccttg gattagaagg gtcactctca
     2101 cagtcaaaat ccatataaat aaaagcgggc ttgactttta tttctgaaag ccactttgcg
     2161 ggatccgtat ttacttgacc gacgtcatta caatctgcgg tcccgagact attaaagatt
     2221 ttataattta ccgaggaccg attggcctct gtgaggttgt tcttgagagt ggcatcaatt
     2281 cctaggctgg ctaattctcc attaatttcg cgaaaatcat cttctttttc actgagtccg
     2341 cgtgagagca aattgatcaa ctgcttccga tcaccctggt caatattgta agccgccatg
     2401 aactccgcca agatcataaa attaaattgg cctacatagc gacagcgatc aatggcctca
     2461 ttcaaggctc tctgtgaggc ttgccgaatc gccgaattaa tactgaaaaa tcctgcaatg
     2521 actggcggag gcgtaaacaa attaattgtc ctgtatgtac tctgatgctt acaagtactc
     2581 tcgttttccg taggcatcgg agaaaatgga gcataggtaa tacaaaaagc cggagcatca
     2641 taataatctt tcgcagtgct cgtttcgcca aacttgggca atccctcaag gtcatcaggc
     2701 cttatctgac gcacaatctt atcataggga tggacattat agtctcccgc tgttcctatc
     2761 tgacgatagc gccacgaaag aagcttccag ctttggcgaa tctgataatt cacgtgaccg
     2821 atggcattca tcatctctgc ctgtttcatc gcaccataat aggcggcgag atccacagag
     2881 ttttgtaggt ttaccttatg atgcaccaaa agccctacat taatgatcat ggcaaagaat
     2941 agaaataaaa cctgaaatat caggg
//
LOCUS       DATBTE010000475         4775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1159194, whole genome shotgun sequence.
ACCESSION   DATBTE010000475 DATBTE010000000
VERSION     DATBTE010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159194"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VIG33_18080"
     CDS             <1..371
                     /locus_tag="VIG33_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272807.1"
                     /translation="KADSGVPSLCSSKDLKDMRAVWIDMERKGFDRARYKKGIGIIGG
                     DCIKPYSLEESAGRRHKEDAPMPSCGIIFILNPELEAEQAAWILDTVGSYYTTKSGIS
                     MRLCSRVSIIGPHPGVTVHN"
     gene            376..1194
                     /locus_tag="VIG33_18085"
     CDS             376..1194
                     /locus_tag="VIG33_18085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02147 family protein"
                     /protein_id="HEY8272808.1"
                     /translation="MQSVYSYQDYRKYLEDFYSLKKKKDPLYSYRSFSQTAGIKSPNY
                     LALVIEGSRNLTTANIQQFAFGLKLRPDEIEYFEALVLFNQSKTAAEQSYYKKRLIHL
                     KWNKPEKIQKSSPAEILKEWFSTGILVLAHGRDMEEAIQKCKIELSISEEAIQNTLQK
                     LIELEYLQFNEGKMQISSQQITFHDPKSISLAQESYLYVQVEQSLKAFRRGYKQKKGK
                     FLSQTLTVPPNSIDEIQKAFTSFVENLTQRMDQQVVFGCEELAQINIQIFRPQF"
     gene            complement(1387..2022)
                     /locus_tag="VIG33_18090"
     CDS             complement(1387..2022)
                     /locus_tag="VIG33_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272809.1"
                     /translation="MIAFAKTVHYAQMKYATILKILLSLIYSCELTAIANDDIIAHGK
                     SPKWHSGTPTEELAKLCNLEKNQNQKILVCNAVISRYSSLASGTVPKEAKSQANYILQ
                     IYDANPNDAQYIFGLHDSHLTLGRIALAEGNLKIAKEELLKSLQVPETPPLQHIGPRM
                     ILARELLEKGEAKSVLSYLEQVEKIWKGEKAKSKLTKWRTEINAGKIPSFQ"
     gene            complement(2253..3587)
                     /locus_tag="VIG33_18095"
     CDS             complement(2253..3587)
                     /locus_tag="VIG33_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEY8272810.1"
                     /translation="MKKFVKAMVASTILKLFTTLKMSIVTSLLFMIIMTLATSKTYAA
                     NMLYKMNFGSDTVLYSPQIAYSTAGWQNIAGTSPSGQVGPISVWGATRSGFDMIADAP
                     VTTSTLGNYMTNQIQQVVGPNGQMVKALFQNVKISTAAPPTWGPSQDAFMIYRGSVDS
                     GDKTDTGDVYYTYWFKFQSDLRSQLGGGDGRNWRVMSEWKTGGLNNTWKGDYRIITHV
                     VLDGSGRLYWNTQGDNNANGISARQTFWQVQNFNVPVPAGEWFKYEVFWHRSSGSDGR
                     YWAAVNGQTIVDHWGPNKGVYNLPINRIMLANNYSGGKTPCQQWVTGLEIWNGFPCGV
                     GASCYGKTGTTTPTPTPAPTTGAVLTAPSGLVATPVSSTSVKLTWNVSQSSAGGVTYW
                     IYRNGVNIAKSTVPSFTDTKAVSGATYGYNLNAHDSAGKVSTPSNYVTVKVP"
BASE COUNT         1338 a          989 c         1057 g         1391 t
ORIGIN      
        1 cgaaagctga ctcaggtgtt cctagtctat gtagctcaaa ggatttaaaa gatatgagag
       61 ccgtgtggat tgacatggaa aggaaggggt ttgacagagc tcgttacaaa aaaggtattg
      121 gaattattgg tggagattgt attaagccgt attcacttga agagtcagct ggccggcgac
      181 acaaagaaga tgctcctatg ccgtcctgtg gaataatatt tattttgaat cctgaacttg
      241 aagcggaaca ggcagcttgg attcttgata cggtaggctc ttactacacg acaaaaagcg
      301 gaatttccat gcgcctctgt tcaagagttt caataattgg acctcatcct ggagtcacgg
      361 ttcataacta atttcatgca aagtgtttat tcatatcaag attatcgaaa gtacttagag
      421 gatttttatt ctttaaaaaa gaaaaaggat cctctgtatt catacagatc attttcccaa
      481 accgcaggaa ttaaatctcc gaattatctt gcacttgtta tcgagggatc acggaattta
      541 actacggcaa atattcagca gtttgctttt ggtcttaagc taagaccaga tgaaatcgag
      601 tacttcgaag cgttggtcct ttttaatcaa agtaaaacgg ctgctgagca aagctattat
      661 aaaaagcgat taatccatct taaatggaat aaaccagaaa aaattcaaaa atcttcgcca
      721 gctgaaatct taaaggaatg gttttcgacc ggcattttgg ttctagcaca tgggcgggat
      781 atggaagagg ccattcaaaa atgtaaaata gaactgtcta tttcggagga agctattcag
      841 aatactctgc aaaagctgat cgaactcgaa tatctacaat tcaatgaagg aaagatgcaa
      901 atttcttcac agcaaatcac cttccatgat ccgaaaagca taagtctggc tcaagaaagt
      961 tatctttatg ttcaagttga acagtcatta aaggcattta gacgaggata taaacaaaaa
     1021 aaagggaagt ttcttagcca gacactgacg gtgcctccaa attccattga tgaaattcaa
     1081 aaagcattta cttccttcgt tgaaaactta actcaaagaa tggaccaaca agtagttttt
     1141 ggctgcgaag aactggccca aatcaatatt cagattttta gaccacagtt ctaacagagc
     1201 ttatatggta ttgcggttac accggggatg gttataaagc ctacggatct tcgacggggt
     1261 atgaaaccag aagatttcga aatttttaat ctgaacactc atctcccttc aatataaaga
     1321 tttatggtga acaggaaata gtcagcggtt agtaagactt tattattatt attattatta
     1381 ttattattat tgaaaacttg gaatcttgcc tgcgttaatt tccgtacgcc acttggtcag
     1441 tttacttttt gccttttcac ccttccaaat cttttcaacc tgttctagat aactaagaac
     1501 gctttttgct tcaccttttt ctagaagctc tcgggcaagt atcattctgg gtccaatatg
     1561 ctgaagaggc ggagtctcag gaacttgaag ggactttaac aactcctctt ttgctatttt
     1621 caaattcccc tctgccaacg ctattcttcc caatgtgaga tgactgtcgt gaaggccaaa
     1681 aatgtactga gcgtcgttgg ggttggcatc atatatttgc aaaatataat tagcctgaga
     1741 tttggcttct ttgggaacag ttcccgatgc caaacttgag tatctggaaa taactgcatt
     1801 acaaacaaga atcttttgat tttgattttt ttctaaattg caaagcttcg ctaattcttc
     1861 agtgggtgtt ccggaatgcc attttggcga cttaccgtga gcaatgatat cgtcgttagc
     1921 gatggccgtg agctcacatg aatagattaa actgagtaag atttttaaga tcgtagcgta
     1981 cttcatttga gcataatgaa ctgtttttgc aaaagcgatc aaggggcact gcttcaaaat
     2041 gaaatgatag agcctcatac cgaggctagt gacgacgcgc gatcacggac ttggtttttc
     2101 agtattggat ctaaaagatc ggtggcgatg atctgagtgt gttttaactt gggctagtca
     2161 caattttaga tcgaacctcg gaggctaatc cccgaggttc gtaatgatta catgtgttgg
     2221 attagagcca actcgtacgt cggttcaatt gattatggga ccttcacggt gacgtaattg
     2281 ctcggagtcg agaccttccc agcgctgtca tgggcgttga gattataacc ataagttgct
     2341 ccagatacag cctttgtatc tgtgaagcta ggtacagtgc tcttagcgat gtttacgccg
     2401 tttcgatata tccaataagt gacaccacct gcgctgcttt gtgatacgtt ccaagtcaat
     2461 ttgacagatg tagaagagac tggagtcgca accaaacccg aaggcgcggt cagaacggca
     2521 ccagttgtcg gagcgggggt tggggttgga gttgtcgtgc ctgttttgcc gtagcaagaa
     2581 gcgccgacac cacaagggaa tccgttccaa atctccaggc ctgttaccca ctgctggcaa
     2641 ggtgttttac cgccactgta gttgtttgca agcatgattc gattgattgg caaattataa
     2701 acgcccttat tagggcccca atggtcaaca atcgtttgcc cattcacagc tgcccaatag
     2761 cgtccatcac ttccactgga gcgatgccaa aacacttcgt atttaaacca ttctccagcc
     2821 ggaacaggga cattgaaatt ttgaacctgc caaaaggttt gccgtgcaga aataccattg
     2881 gcgttattat cgccttgagt gttccaatat aaacggccac tcccgtctaa aacaacgtgc
     2941 gtgatgatac gatagtcgcc tttccaggtg ttgttcaggc cgcctgtttt ccattctgac
     3001 ataacccgcc agttcctgcc atcgccacca cccagttgac tgcgcaaatc tgattggaat
     3061 ttgaaccaat aggtataata aacatcaccc gtatcggttt tatcgccaga gtcgactgag
     3121 cctctataga tcatgaaggc atcttgggag ggaccccaag tcggaggagc ggcagtgctg
     3181 atctttacgt tttggaagag agccttgacc atttgtccgt tgggaccaac cacttgttgg
     3241 atctggttgg tcatataatt gcctaaggtc gaggtggtta ctggcgcgtc ggcgatcata
     3301 tcaaaaccag atctagtcgc accccaaacg gagatgggtc cgacttggcc gctgggagac
     3361 gtgccagcga tgttttgcca accggcggtg ctgtaagcaa tttggggcga gtaaagaact
     3421 gtgtctgatc caaaattcat tttatagagc atattcgctg cataagtctt tgacgtggcg
     3481 agtgtcatga tgatcataaa taatagactg gtcactatac tcattttcag cgtagtgaaa
     3541 agttttaaaa tagtgctggc gaccatcgcc ttaacaaact ttttcattag gttacctccg
     3601 gtagatttcg gttatttcat attgagcctg cggaagttct ttttacctta atcaaataca
     3661 aagcactttt cggtttggag gaagtcgtcg acttaactcc aaaggacttc tatctacagt
     3721 cttgctacag ttgtgtcttg caaaatggag tcctgccaaa gcgaaaataa agcacttacg
     3781 atctgacttt ttaactaaaa aattgacatc tcatcgagag actaatcgag attagtggca
     3841 aaaacgactc aaatggataa aagcattcaa tctcctataa taactgcttc ttaatgagat
     3901 gatcttcata caaccaagcg ctcaaatcat ttttaaagac aaatggagaa agcgcaaaaa
     3961 ttcgcggaca tttctgtaga ttgaattgtc aacaatcatc attttgacat tttcgtgatc
     4021 tgatggtatt gcaaattccc agccttattt gccagtcgag tattaaatag tcctgatgga
     4081 agtgtaaagt aaaagaatca gtcccgaagg gccgactctt ttttactaat aagttatttt
     4141 caagtgcctt ggagttagta aaggcctata gaaattcctg ccgcgccatc tagatcgatt
     4201 gccacgccgt ctaatgcctg tgaaattttg aagtggtgta ggacattgtt cgtaatcctc
     4261 cagttcttaa cccactgagg aatcagaggg cctgatgcga cgaccacact tttgcctgcc
     4321 caaatctggt tgccgctgat ttttccaccc gaggagatgg cattgttgct cgatcctcca
     4381 atgaacgcga ttccgatgtt ttcgccttca aagctgtttc cggtaatcgt aattttgccg
     4441 aggtcatagg aggccgtggc agatttttcc tggggcgcga tggcaacgcc gttgcgaatg
     4501 gtcgattgct cactgccacc gactcctgct gcgttaagtt tgctggcttt aattgtaccg
     4561 tctccggagg aggcaatgat gtgcgcctat gtatcggcat agaccgcaat cttcttaatc
     4621 taaaacggtg tctcaaaaga tatagggact gatcgagagt ccgatcgcag caatactgga
     4681 tgaggatttt ctaggtttct gagttttacc actttttttc ttttatttcg agagaggatt
     4741 cttgataggc cagggttgga gtgagccgcc gccgg
//
LOCUS       DATBTE010000476         1714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2114592, whole genome shotgun sequence.
ACCESSION   DATBTE010000476 DATBTE010000000
VERSION     DATBTE010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2114592"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VIG33_18100"
     CDS             complement(<1..613)
                     /locus_tag="VIG33_18100"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HEY8272811.1"
                     /translation="MKITETNLGFLIESPKFTALFGGEKAQILNLKSSFPDYDFIRIK
                     QTHGDTIVHSNDPKLDYQVEADSHFTELSKTALCISTADCIPLLVYDKKNDIIAAIHA
                     GWRGVANRIVSKTLQHLQTQGTKVENTQVIIGPHIQMPSFEVSFEVRDEILSSIDFSP
                     HEKESLYHRNISNEKSLVDLNQVIRTQLQSGGVTFDNLHNIHID"
     gene            complement(610..1611)
                     /locus_tag="VIG33_18105"
     CDS             complement(610..1611)
                     /locus_tag="VIG33_18105"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY8272812.1"
                     /translation="MILSFKCTEELQGLRLDKALGLRPEIETRSRASHLIEDGLVTVN
                     GQKVKASVILKPGDHIEIQLPEPEPTDLIPYDFPLDILFEDDHLIVVNKPSGLVVHPA
                     AGHTQDTLVNALIYHVKNLSMKFGEQRPGIVHRLDKETSGILVIAKNDKAHENLTQQF
                     KQRSIHRIYYAVCMGVPKPQAGQIQSYLARHPVDRKRYASVLNSGVGTDKKIQTTKEP
                     IPPIGKWAVTHYEVLKNHSGLSYCKLKLETGRTHQIRVHLAEKGTPIAGDNLYGADKK
                     IKSIAAKKIQADLKNLNRFLLHAAELGFLHPLTGEELLFTQGWPEDIQAQIQQWGLQ"
BASE COUNT          438 a          346 c          391 g          539 t
ORIGIN      
        1 tatctatatg tatattgtga agattgtcaa aggtgactcc accgctttga agctgagttc
       61 tgatgacctg atttaagtct actaaagatt tttcattact gatatttcga tgatagaggc
      121 tttctttttc atgagggcta aagtcaatgc tagaaagaat ctcatcgcgc acttcaaagc
      181 tgacttcaaa gctgggcatt tgaatatgcg gcccaataat gacttgagtg ttctccactt
      241 tcgtaccttg agtttgcaag tgttggagag tttttgaaac aattcgattt gcaactcctc
      301 tccaacctgc gtgaattgcg gcaatgatat catttttctt atcatacaca agcagcggaa
      361 tgcagtcggc ggttgaaatg caaagggcag ttttcgagag ttctgtaaag tgggagtcgg
      421 cttccacttg ataatctagt ttgggatcat tcgagtggac aatagtgtca ccatgagttt
      481 gtttgatgcg aataaagtca taatctggaa aagagctttt caaattaaga atctgagctt
      541 tttctcctcc gaaaagagct gtaaatttgg gggattcaat aagaaaccct aaatttgttt
      601 ctgtaatttt cattgtaaac cccattgttg tatttgggct tggatatcct cgggccagcc
      661 ctgagtgaaa agaagttctt cacccgtgag ggggtggaga aagcctaatt ccgcggcatg
      721 caagagaaag cgatttaaat ttttgagatc tgcttgtatc tttttcgctg caatagactt
      781 gatcttttta tcagcgccat aaagattatc tcccgcaatg ggggttccct tttctgcaag
      841 atgaacgcgg atctggtgag ttctgccggt ctcgagcttg agtttgcaat agctgagacc
      901 agagtgattt tttaaaacct cgtaatgagt cacagcccat tttccgatgg gcggtattgg
      961 ctctttagtc gtctggattt ttttatctgt ccccacgcct gaatttagaa ccgaggcata
     1021 tcttttgcga tccaccggat gtcttgcaag gtaagattga atttgacccg cttgaggctt
     1081 tggcacgccc atgcacactg catagtagat acggtgaatg cttcgttgtt tgaactgttg
     1141 agtgagattt tcgtgggcct tatcgttctt tgctataacc aggatgccac tggtttcttt
     1201 atctaaacga tgaacgatcc ctggacgctg ctcaccaaat ttcatggaaa gatttttcac
     1261 atgataaata agggcgttta ccaacgtatc ctgagtgtgt ccggccgccg gatgaaccac
     1321 taatcccgaa ggtttattga cgacaatcag gtggtcatct tcaaagagaa tatctaatgg
     1381 gaaatcgtaa gggatgagat ctgtgggttc cggctccggc agctgaattt caatatgatc
     1441 ccccggtttc aaaatgaccg atgcttttac tttttgcccg ttaacggtga caagaccatc
     1501 ttctatcaag tgggacgctc gggagcgcgt ttcaatttcc ggtcgcaggc ctaatgcctt
     1561 atcaagtctc agtccttgca gctcttcagt gcacttaaaa gagagaatca tttttgagga
     1621 gtggtttctt tcaataattt catataagaa tcagcaactc ttttttcgtc gagtcccagt
     1681 agcttagata tttgtacgac atagccacgg atga
//
LOCUS       DATBTE010000477        12827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_271955, whole genome shotgun sequence.
ACCESSION   DATBTE010000477 DATBTE010000000
VERSION     DATBTE010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 12827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12827
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271955"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VIG33_18110"
     CDS             <1..301
                     /locus_tag="VIG33_18110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272813.1"
                     /translation="QMTTTVFLQATLDASYNLNSCYALGLADSLWQISPANMADIYYP
                     SGNVGIGVGTPLSRLHIVDSGSQPGSFTGTTAGTLRVWGGNFVDGGFTNIVLYPR"
     gene            585..896
                     /locus_tag="VIG33_18115"
     CDS             585..896
                     /locus_tag="VIG33_18115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272814.1"
                     /translation="MRTAKLQSILVFATLAIILSACSMEASIQNLAEEVKSLKAPGQA
                     HGITSGSGQMKKDLTSGTYKVSASIGVVGGTNKVNNSLYHQTASGFKVYSTVQGAIVS
                     Q"
     gene            967..1398
                     /locus_tag="VIG33_18120"
     CDS             967..1398
                     /locus_tag="VIG33_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272815.1"
                     /translation="MKPWVILISASFFVANATKAQDLNIVDVRRNITLSDEETVYKDF
                     YINGGEAEGLKKNLIVTAVRKILIRDANGSQSYGEIQVPVGQLRVIATYGRIAVAREY
                     KLLSRDELPMLEQTGLMNGDKIDLKGSFIDNNKPVHKKKAQ"
     gene            1458..2363
                     /locus_tag="VIG33_18125"
     CDS             1458..2363
                     /locus_tag="VIG33_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEY8272816.1"
                     /translation="MNSYFRIRLSHVSTLLEDEVTSCSFEHGATGVTEALVFIQPDLT
                     YDPDLVPMKSHDLDVFFLEKPTPEFFSALAELDQNIQWNIFEEENKDWLEEWKKGFKP
                     FQLVGPFWIIPSWLQPPPECEYPIYIDPGMAFGTGTHATTKMASYFVHKLAEKYKEEI
                     PQWALLDVGTGTAILAMLALKSGFGLVSGIEIDPEARRVAKENVKINKMGEIDISEAL
                     LEEVRGEYDVVIANIIDGVLINIRKDLLRVLKPGGFLFLTGILTERDSLFFEKFIEAS
                     NLKVLRRLEKEEWVGYWVQKPEVEE"
     gene            2366..3124
                     /locus_tag="VIG33_18130"
     CDS             2366..3124
                     /locus_tag="VIG33_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEY8272817.1"
                     /translation="MRRYWIEKKDLVGEHVHFVNETFHHIFDVCRQKVGSKFEVLNEE
                     SKAYLVEVLSIDKKSAEARILEKRTIPPLPEPHIHLALAVSRFPVMDAIVEKAVEMGV
                     KSIQPLFTEFSFVRTSSALSANKVERWSKIVRSATQQSGRGDLMSITPALPLDGFLKS
                     FNQKSSVMGLFAYEGQSTLSIKDYVTEKKKEMRLIKEIWIFVGSEGGFSYDEVEKLRS
                     LALHPVTLGSQVLRVETACMALVSVLKYDFDLMC"
     gene            3136..3906
                     /locus_tag="VIG33_18135"
     CDS             3136..3906
                     /locus_tag="VIG33_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloendopeptidase"
                     /protein_id="HEY8272818.1"
                     /translation="MDPFEEFEFKPLTEGLGFHKKKTQKPTDLDSSFEVTGPGKSLNP
                     SGLSLLEEEAVDPLRPPLPRKSSMPTIVESETTPSSSAVDEILKTLQKNRHLDFEKKA
                     TVKTTAAPAPKVDEYKPDVWNFSSALLDGMLVIAASLLCMIIVLIVTKADLIANLSTP
                     DEGGMIYLSTFALVATVSFIYLLVNRIFVGSTPGEWAFEQRIGQPQEMNSATYSLRIL
                     ARSALVIATGFVVLPILSLILKKDIAGEITGALLLKKV"
     gene            3924..4379
                     /gene="rfaE2"
                     /locus_tag="VIG33_18140"
     CDS             3924..4379
                     /gene="rfaE2"
                     /locus_tag="VIG33_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEY8272819.1"
                     /translation="MKFEKISSALALLKAQNKKIVFTNGCFDLLHVGHVRYLQEAKKL
                     GDVLVIGVNSDASIKRLKGPARPVQTEEDRAEILAALGCVDFTVIFTEDTPKNLIEAV
                     KPDVLVKGGDWKIDQIVGSDFVLMNGGKVLSLQFVDGKSTTKLIEKAQK"
     gene            complement(4391..5299)
                     /locus_tag="VIG33_18145"
     CDS             complement(4391..5299)
                     /locus_tag="VIG33_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272820.1"
                     /translation="MNVMDENRPALKLKYRLNCRLFGNEGQTLISVLIGAAVMIVSML
                     AFMGMMSAQQKEIRAMSEKLAALDLERLMIAAFSDPAICTAELSNSALNASAPYVVNV
                     GSLSNYVINLNSIHSNAAPASPVLISAGALVTPTSSLKVSTIKVKNFTSNSGTGTNYL
                     AEIEISFDPASLVRSIKPITLKVSITTDATQKITTCMGSFGASSTTEVITIYQCPNVP
                     NATLLNPSNGYVNVTLIQTGPLITSVSGCQGGSTEVGALVTPCSGQLSTHSICQAVGI
                     AGNALANTSTSRYCSIACTALGKLAN"
     gene            complement(5415..6083)
                     /locus_tag="VIG33_18150"
     CDS             complement(5415..6083)
                     /locus_tag="VIG33_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272821.1"
                     /translation="MSLPTGLNASDRETTLGILGFGSAIKLLSSPYPLGGYDGVEVGL
                     SSEYIPSSDLASLGSKSTSGNDINYFSLVLGKGLFYNIDILVQFVPLPQEEFISGFGG
                     QLRWSFYETKFMPLALSLVFHGSSMNFNNLLGCETTGMDLMGSVNMRDVSLFFGAGQA
                     RSVGSFIGGTGGITDSGNAETSDISSPHTVFGISIRMSNVFLALEVDRYVHSTYAGKM
                     GFRF"
     gene            complement(6176..7246)
                     /locus_tag="VIG33_18155"
     CDS             complement(6176..7246)
                     /locus_tag="VIG33_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272822.1"
                     /translation="MGNTNRYVSGMILAIVVVAFGSFYFLHGAIRSIRSNTEFDMSYS
                     MPRPKGSLYNFIFGLDGREIDRKEIDPFKDRMAAQKAKAEAAGVGKELPKIDPKKATP
                     GVAKAAAPAATKKPEVKVNVVDAEPQSPLTAGEVAEQPLPNAGANPQGVVSAQGHGEA
                     KKEADTMSAAQWRALILGQPTKENVMKLIAAFNDKEVESTTLYLIMNDLMQSSNTETQ
                     GLGLMIGQNIPSLKSFSIVSENYEKFDAATKKDADAYLMSYMQGSKLPILALALQSGD
                     NQVVARAAQVMVAGLEQAKNGKNTNSNNREDSGRGVIAASTSKVYAQFISIFQGLVKS
                     SDSTVAGLAQNALTQIQALSNT"
     gene            complement(7451..7837)
                     /locus_tag="VIG33_18160"
     CDS             complement(7451..7837)
                     /locus_tag="VIG33_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEY8272823.1"
                     /translation="MSKVDIDRPSSWKFYRKGMCDNCWGGCCTMPVEVKLSDLIHLQV
                     VTEDEALGSIKKLAKRLMREGIVSSYRQGTELFMLTQKYGRDCYFLNSATRLCTVYER
                     RPGVCREFPNIGPRPGFCPSINKKDR"
     gene            complement(7848..8945)
                     /locus_tag="VIG33_18165"
     CDS             complement(7848..8945)
                     /locus_tag="VIG33_18165"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY8272824.1"
                     /translation="MKKNIYIIIFLMSSSACTNSLHHEIRQSISANQKARSLLEWETA
                     EENPKKVFSRLREEFKTNSKLGSTVCEELLLLKGQDLSLFEEEINNPANGALLKECII
                     SLKKKLEEYWLLQKKPFQSSGLNFSFEPRIEKRDLSKGYRASSGDVQSKELIITFDDG
                     PHSDYSPKILDILKSVNAKAMFFPLGPHVRGNPAIVYRAATEGHVIGSHSITHRCLAD
                     NTVCAKANGGKPLPYDQAVAEIRGGHQAVYEVLGFVDPFFRFPYGESSFQLSNFLASR
                     QVGQFYWSIDSGDWRSQTNEELLKNTLAQVDKNQRGIVLFHDTHRRTLEILDEFLRAI
                     YEKGYTLVVLQSTDEKARYNSQLVTKHWDIP"
     gene            complement(8958..10295)
                     /locus_tag="VIG33_18170"
     CDS             complement(8958..10295)
                     /locus_tag="VIG33_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272825.1"
                     /translation="MENLSERKAESIFQPRDFVVLLKRHGKLFGGIFIVFLTVSLLVY
                     IFKIPYVGRGRLLVNDSQNSSLQAFSMAYFGMTKSVVDGKKGNTQIGKQVEVLRTREF
                     YEKLLQRLDERGKSLLLNVDEQLAYQSIKDKYLMQAFKDESSRQNFIVKLDSWSQAKL
                     ESDYEIRISFSTPSKELSLFLTNTALELSSEYLRDREMDEINEVEKFINDQKQRVDKN
                     IKSLTQELADFQIQPENLISLTSRDKMGEYLSDLMVRINEAKLKVSENKKDMEFLQED
                     SGKIGDKYEGSFLYGVGGRLEALRLENKMLESRMVQLKESVDRIGKYMKVLPVASQML
                     EDRRKKSELEYIKYKELSETLAKVEAQKLSVRDRFEILERARPDNTSPQVDLGTLIFL
                     SLVMSLVFGLTFIYLQYLWKPVVLQKEALRNIMTFDNSAPESPFHLGNHASLE"
     gene            complement(10401..11255)
                     /locus_tag="VIG33_18175"
     CDS             complement(10401..11255)
                     /locus_tag="VIG33_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HEY8272826.1"
                     /translation="MSFWLLLQVLVDLILIAGSVGVWIRFQRPPKDDPRLSKGLQLLQ
                     SKIAVLEDLSDRTEVQVQQLTSLLEQKVRQIQQQVQHSDKQLLKIEQSMQKSLEVAKI
                     FQDRIPHNEIVERQSTIKYVKAARLAHQGKSAAEIASQVDLSLGEIEMIAQVNKDQLM
                     FCEESLPEWAQASTQPTPPEYAESNEVDSFQESRIDMEAPLSREIPVDLTKAFEVPTA
                     DQEALKKLGDAFRQACETANHLQQVTQGFVEDNKSPVTSEDTSQTTDAAGRVIRPVKF
                     PRIASTKF"
     gene            11444..12649
                     /locus_tag="VIG33_18180"
     CDS             11444..12649
                     /locus_tag="VIG33_18180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272827.1"
                     /translation="MTSSISILSIWTLKRLTILVLSAVLLPSCGNVRFNADAPVQAAS
                     PGTTGSGTVNDNGGTAPGTSPTPTPSAPRIVTYSSVVPPNANKVDILLIIDDSGSMKT
                     DQLKLANKLSGFASQLESSSAPMDWQMCVTVTRDQLISGKYYWGASVNWVGYSPASGP
                     QYVLKKGTTGLNDIFTKTINAIGAGVPGSGDERAIKAAYHHFYNGEPGVAGTSGCYRK
                     DAAVAVIVISDENERSVGGDCTSIKSNMNEDPASCLALEAEDQPSTLLTQAKSIFGPN
                     ARFSFNSIVVGDAVCESTQDQTADEKGQRSPSHQGLKYIEISKLTNGGVSSICENDFS
                     TNLNIFKDKILNSLSSLTLECSPIAGSLSVSINNVPTTDYVLMGANLKFNNAVVEGKT
                     VSMTYKCSQ"
BASE COUNT         3532 a         2906 c         2774 g         3615 t
ORIGIN      
        1 acaaatgacg acgacggttt ttttgcaagc gactctggat gcttcctata atctcaatag
       61 ttgttatgcc ctgggacttg cggattcact gtggcaaatc tctcccgcga atatggcgga
      121 tatttattat cccagcggca acgtcggtat tggggtgggg accccccttt ctcgattgca
      181 tatcgtggac agtgggagcc aacccggctc atttactggc acgactgccg gaaccctgcg
      241 tgtgtggggc gggaatttcg tggatggagg gttcacgaat atcgtcctgt atccccggta
      301 gcaggggctg ttttagttgc cgctcaagga tattttgcca atgtttcagc cgtcgtttat
      361 cttaatggga cgacagattc aaccagttta agtattgtca actttagcgg tggtacggtt
      421 actggtgaag tagtcaaaat gacttggagt aagatccctt aaagacgctt gttcaacgga
      481 gcaactcaac tcattcgcgt attcgtctca aaacaggata actctttaac ttttaaagtt
      541 tgttaatttt ttatccgata agtcaggcaa tgggaggctt tcaaatgaga accgctaagt
      601 tacaatccat cttggtattt gcaactctag caattatttt gagcgcctgt tcgatggaag
      661 cctctattca aaatcttgcc gaagaggtta aatccctaaa agccccgggg caagcccatg
      721 gcattacttc cggaagcggc cagatgaaaa aggatcttac aagcggaacc tataaggttt
      781 ccgcctccat cggcgtagtg ggaggcacta ataaggtgaa taatagcctc taccaccaga
      841 ctgcctccgg atttaaggtt tatagcaccg ttcaaggtgc cattgtttct cagtaaagct
      901 ggaccaaggg gggttgttaa gttttaaaat gaaacaaccg acaaactcct caaggaaggg
      961 aatcctatga aaccttgggt gattttaatt tcagcgtctt tttttgtcgc caatgcaacg
     1021 aaggcccaag atctcaatat agtggatgtt cgccgaaata tcactctttc cgatgaggaa
     1081 acagtctata aagattttta tataaacggc ggcgaagccg agggccttaa gaagaatctt
     1141 atcgtcaccg ctgttcgtaa aattctgatc cgagatgcga atggttccca aagctatgga
     1201 gaaatccaag tgcctgtggg acaactgcgc gtgattgcca cttatgggag aattgccgtc
     1261 gcccgtgaat ataaactcct ttcacgtgat gagctgccta tgcttgaaca gacaggattg
     1321 atgaacggag ataaaatcga tcttaagggt tcttttattg acaataataa gcctgttcac
     1381 aaaaagaaag ctcagtagat taagatatta actccctgct ggacccgcga gaaattttag
     1441 ggcacaacta cgggtctatg aattcatatt ttcgcatccg tcttagccac gtttccactc
     1501 ttcttgaaga tgaggtcacc tcttgctcct ttgaacatgg agccaccggt gtcactgaag
     1561 cccttgtctt cattcaaccc gatctgacct acgatccaga tttagttccg atgaaaagtc
     1621 atgatcttga tgtgtttttc ctggaaaaac ctacgccgga gtttttctca gcgcttgccg
     1681 agctagatca aaatatccaa tggaatatct tcgaagaaga gaataaagat tggcttgaag
     1741 aatggaaaaa aggctttaaa cctttccaat tggtagggcc cttttggatc atcccttcct
     1801 ggcttcagcc tcctccggag tgtgaatatc caatttatat cgatcccggc atggccttcg
     1861 gaaccggaac ccatgcaacg actaaaatgg cttcctactt tgttcataaa ctggctgaga
     1921 aatataaaga agaaattcct cagtgggccc ttctggacgt gggcaccggc acggccattc
     1981 tggcaatgct cgcgctcaag agcggctttg gtttggtttc aggaattgaa attgatcctg
     2041 aagctcgtcg tgtcgctaaa gaaaatgtga aaatcaataa gatgggtgaa attgatattt
     2101 cggaggccct tcttgaagag gtgcgtggag aatacgatgt cgtgattgcg aatatcattg
     2161 atggcgttct gatcaatatt aggaaagatc ttttacgtgt tttaaaacca ggcggttttt
     2221 tatttcttac cgggattctt acggaaagag atagtctctt ttttgagaaa ttcattgaag
     2281 cttctaactt gaaggtgctg cgtcgtcttg aaaaagaaga atgggtggga tactgggtgc
     2341 aaaagcctga ggtcgaggaa taaaaatgcg tcgctattgg attgaaaaaa aagacctcgt
     2401 tggcgaacac gttcactttg taaatgaaac ctttcatcat atcttcgacg tttgccgcca
     2461 aaaagtaggc tctaagtttg aagtgctgaa cgaggaaagc aaagcttact tggtggaagt
     2521 tctctccatt gacaaaaaat ccgccgaagc tcgcatcctc gaaaaacgca cgattcctcc
     2581 attaccggaa cctcatattc atctcgctct agctgtttcg cgctttccag tcatggatgc
     2641 tatcgtcgaa aaagctgtgg aaatgggggt taagagcatc caacctctct ttactgaatt
     2701 tagctttgtt cgcacctcca gtgctctgtc cgctaacaaa gtcgaaagat ggagcaaaat
     2761 cgtacgctcc gcaactcagc aaagcggccg tggtgatctc atgtcgatca cacctgctct
     2821 gccactggat gggtttttaa aaagctttaa ccagaagtcc tcggttatgg gtctgtttgc
     2881 atatgagggc cagtcgactc tcagcattaa ggattatgta actgagaaga aaaaagaaat
     2941 gagacttatc aaagaaattt ggatctttgt gggcagcgaa ggtggcttct cttatgatga
     3001 ggttgagaaa ttgcgcagcc tagcccttca tccagtcact cttggctcgc aagttttgag
     3061 agtggaaacg gcttgcatgg ctctagtctc cgtcttaaag tatgattttg atctgatgtg
     3121 ttgataggag atggaatgga tccatttgaa gagtttgaat ttaagcccct cacggaaggt
     3181 ctaggctttc ataaaaagaa aactcaaaaa ccaacagatt tggattcttc ttttgaagtt
     3241 acagggccgg gtaaaagttt aaacccaagc ggcctctctc ttctggaaga agaagcggtg
     3301 gatccccttc gtccaccact ccctaggaaa tcatcaatgc cgacaatcgt ggagtccgaa
     3361 accactccct cctcttccgc cgttgatgaa atccttaaaa ctctgcaaaa aaaccgtcat
     3421 ttagatttcg aaaagaaagc cactgtaaaa accaccgcgg ctccagctcc taaagttgac
     3481 gaatataagc cagacgtttg gaatttctct tcagcccttc ttgatggaat gctggtcatt
     3541 gccgccagcc tgctttgcat gatcattgtt ttaatagtca ccaaagccga tttgattgca
     3601 aacctctcca ctccggatga gggtggtatg atttatcttt ctacctttgc tttagtcgcg
     3661 accgtcagct ttatctatct tttagtgaac cgtatatttg tgggttccac tcccggggaa
     3721 tgggcttttg aacaacggat cggtcaacct caagagatga attccgcaac ctactcttta
     3781 agaatcctgg cccgcagtgc tctcgtcatt gccactggat ttgtggtgct gcctattttg
     3841 tccttgatcc ttaaaaagga tatcgcgggc gaaatcaccg gcgccctact gctgaaaaag
     3901 gtttagtttc catgggacag atcgtgaagt ttgaaaaaat cagctccgct ctggcccttt
     3961 taaaagctca gaataaaaaa atagttttca ccaatggctg ctttgatctg cttcatgtcg
     4021 gccatgtccg ttatttacaa gaagctaaaa aacttggaga tgtgctcgtc attggggtaa
     4081 acagtgatgc cagcattaaa cgcttaaagg gtccagcccg accggtgcaa actgaagaag
     4141 accgggcaga gatactcgca gctttgggct gtgtggattt caccgtgatc tttacagagg
     4201 acactccgaa aaatcttatt gaagccgtga agcccgatgt tttggtgaag ggcggcgatt
     4261 ggaaaataga tcaaatcgtc ggttcagact tcgttctcat gaacggcggc aaagtcctct
     4321 ctctgcaatt cgttgatggc aagtccacca caaaactcat cgaaaaagct cagaagtaaa
     4381 aaaataagga ttagttggcc aacttaccca gagcggtgca agcaatgctg caatatctgg
     4441 aagtagaggt gttggctaag gcattaccgg ctatgcctac agcctgacag atactgtgag
     4501 tcgataattg accagaacaa ggagtgacca aagcacccac ttccgtagag cctccctgac
     4561 aacccgaaac actcgtaata agaggacctg tttgaataag agtaacatta acatacccat
     4621 tactgggatt aagcaaagtc gcattgggaa cattgggaca ttgataaata gtgataactt
     4681 cagtggtact ggaggcccca aaactgccca tgcaagtggt aattttttga gtggcgtccg
     4741 tggtgatact cactttaagt gtgataggct tgatcgagcg cacgagactt gctggatcga
     4801 aactgatttc aatctcagcc agataattgg ttcccgtacc ggagttgctt gtgaaatttt
     4861 tgaccttaat tgtgctcact tttaaagaac tcgtaggagt gactaacgct cctgcggaaa
     4921 ttaaaactgg tgaagccggt gccgcgtttg aatgaatgga gttgagatta atcacatagt
     4981 tggatagact ccctacattg actacatacg gagcactggc attgagtgca ctgtttgaaa
     5041 gctccgctgt gcaaatcgct ggatctgaaa aggccgcaat cataagcctc tctaaatcca
     5101 aggccgccag tttttctgac atggctcgta tctctttttg ttgggcgctc atcatcccca
     5161 taaatgccaa catgctcact atcatcaccg cagcaccgat aaggacagaa atcaaagttt
     5221 gaccttcatt accaaaaagt cggcagttta gtcggtactt taattttaga gctggccgat
     5281 tctcatccat aacgttcata ttcccctggg atattgtaac aaaaccacaa gaatttacct
     5341 cgatttctca aattattttc accgggcttg ctttccatgt tatgcagatc ccgcaatgag
     5401 actcagccgc acctttaaaa ccggaagccc atcttcccag cataagtgga gtgaacatag
     5461 cgatccactt cgagagctaa aaatacgttc gacattctaa tactgattcc gaaaacggta
     5521 tgcggggaag aaatatccga ggtctccgca tttccagaat ctgtgatacc acccgttccg
     5581 ccaatgaaac tccctaccga acgagcttgg cccgcaccaa aaaataaaga aacatcccgc
     5641 atattcaccg accccatcag atccattccc gtagtttcac atcctaacaa attattaaag
     5701 ttcatgctgg agccatgaaa aaccaaactg agggccaagg gcataaattt ggtttcgtaa
     5761 aagctccaac gcaattgccc tccaaagcct gagatgaatt cctcttgagg caggggaacg
     5821 aattgaacca gaatatcgat attataaaaa agccccttac ctagaaccag actgaaataa
     5881 ttgatatcat ttccagaagt cgacttactc cccaagctcg ctaggtctga ggacggaata
     5941 tattcgctgc ttaatcccac ttcaactcca tcgtatccgc ccaaaggata gggactcgaa
     6001 agaagtttaa tggctgagcc aaagcctaag atacctaaag tggtttcgcg atctgaagca
     6061 tttaaaccgg ttgggagcga catggcggcc ggagtccgta ctgaaagaaa aaaaataaaa
     6121 agagttatga caaatctcaa catctcttca tcaaaaacca attcagggaa ttagtctagg
     6181 tgtttgagag ggcctggatt tgagtcagtg cattctgcgc aaggcccgca actgtactgt
     6241 cagagctttt aaccaaacct tggaaaattg aaataaactg agcatagacc ttactcgtac
     6301 ttgcggcaat aactccgcgt ccactatctt ccctattatt gctgtttgta tttttcccat
     6361 tttttgcctg ttccagacct gccaccatca cttgtgcggc ccgtgcaacg acctggttat
     6421 ccccagactg aagggctaaa gcaagaattg gtaattttga accttgcata taagacatca
     6481 aataagcatc cgcgtctttt ttagtggctg catcgaattt ttcataattt tcagaaacaa
     6541 tgctaaagct tttaaggctt ggaatattct gaccaatcat caaaccaaga ccttgagtct
     6601 cggtatttga actttgcata aggtcattca taatcaagta taaagtggtg gattccactt
     6661 ctttatcgtt aaaagccgca ataagcttca tcacattttc ttttgtaggt tggccaagaa
     6721 tcagagcacg ccattgagcg gcactcatcg tatcagcctc tttcttcgct tcgccatgac
     6781 cttgagcgga aacaactcct tgaggattcg caccagcatt tggcagcggt tgttcagcga
     6841 cttctcccgc cgtcagcgga ctttgcggtt ctgcatcgac tacattcact tttacttcag
     6901 gtttttttgt agcggcaggt gcagcagctt ttgccacgcc tggagtcgct tttttaggat
     6961 caatcttagg aagctcttta ccaactccgg cggcctcagc cttagccttt tgagcagcca
     7021 ttctatcctt aaaagggtcg atttctttac ggtcgatctc acggccgtct aaaccaaaaa
     7081 taaaattata aagtgacccc ttaggacgtg gcatgctgta agacatgtca aattcagtat
     7141 tgctacgaat gcttcgaata gctccgtgca aaaaatagaa actaccaaag gcaacaacca
     7201 caatcgccag aatcattcct gatacatatc gatttgtgtt tcccataaat ggcctccctt
     7261 ggttacaatt attcaataag ctaagtgtgt gccaatagag atggattatg gcagagctga
     7321 gagggctact ctgagaatat tttcactcca gggcaattta cgcgccttga tgtccaactt
     7381 ttagacactg ttgaatttct taccggtatg ttcagtcttc atatgacagt aggccgattt
     7441 ttcctggcgt ttaacgatcc ttcttattaa tagaaggaca aaagccagga cggggaccga
     7501 tgttgggaaa ctcacggcag actccggggc gtctttcata aacggtgcaa agacgagtgg
     7561 cggagttgag aaaatagcaa tcacgaccat atttctgagt gagcatgaaa agctccgttc
     7621 cttgccggta gctgctgact atgccttctc gcatgagtcg ctttgcgagc tttttaatag
     7681 agcctaaagc ttcatcctcg gtgaccactt gtaagtgaat caaatcacta agtttcacct
     7741 ccaccggcat cgtgcaacag ccgccccaac agttgtcaca cataccttta cgataaaatt
     7801 tccagctcga cggtcgatcg atatccactt tactcatctg gcctatacta aggaatatcc
     7861 cagtgctttg tcacgagctg actgttgtaa cgggccttct cgtctgtaga ttgtaaaaca
     7921 accagagtat agcccttttc ataaatagcc ctcaagaatt cgtccaaaat ctccaaagtt
     7981 cttcgatgag tgtcatgaaa caaaactatt cctcgttgat ttttatcaac ctgggccaga
     8041 gtgtttttca aaagctcttc attggtttgg ctgcgccaat ctcctgagtc gatgctccaa
     8101 taaaactgcc ccacttgtct gctggctaag aaattcgaca gctggaagct tgactctcca
     8161 taggggaaac gaaaaaaagg atccacgaaa cctagaacct catagactgc ctgatgcccc
     8221 ccacgaattt cagccacggc ctggtcataa ggaagcggct ttccgccatt ggctttggcg
     8281 cagacggtat tatccgctag gcatcgatga gtgatggaat gactgcctat aacatgcccc
     8341 tctgtcgcag cacgatacac aatggcggga tttccacgta catgcggacc gagaggaaaa
     8401 aacatcgcct tggcattcac cgacttcaaa atatccaata ttttcggaga atagtcggag
     8461 tgcggcccat catcgaaagt gataatcaat tctttagact gaacatcgcc actggaagcg
     8521 cgataccctt tggaaagatc tcttttttcg atgcgaggtt cgaaactaaa attaagccct
     8581 gaggactgaa aaggcttttt ctgcagtaac caatactcct ctaatttctt ttttaaggaa
     8641 ataatacact ccttcaataa agctccgttg gcagggttat tgatttcttc ttcaaaaagg
     8701 gaaagatctt gtcccttcag cagaagaagc tcttcacaaa ctgtggatcc cagtttggag
     8761 ttggtcttaa attcttcacg aagtctcgaa aaaacttttt ttgggttctc ttcggcggtt
     8821 tcccactcga gaaggcttcg cgccttttgg ttagcgctta tagattgtct tatttcatgg
     8881 tgaagtgaat tcgtgcaggc ggaggaactc attaaaaaaa taattatata aatatttttt
     8941 ttcatatctt ttcccattta ttcgagagag gcgtggtttc ctaaatgaaa cggagactca
     9001 ggggcggaat tatcaaaagt cataatattc cgcagagcct ctttttgtaa taccacaggc
     9061 ttccaaaggt attggagata aatgaaagtc agaccaaaaa ccaaagacat caccaggctt
     9121 aaaaaaatca aagtccctaa atccacttga ggactggtat tgtctgggcg agcacgctca
     9181 aggatttcaa aacgatcccg aactgataac ttctgtgcct cgaccttggc gagagtttcg
     9241 cttaactcct tgtacttgat atattcaagc tctgattttt tacgccggtc ttcgagcatc
     9301 tgcgaggcga ccgggagaac tttcatatat ttgccaatcc ggtccaccga ttccttaagt
     9361 tgtaccatgc ggctttcgag cattttgttt tccaatcgca gggcttcgag tcggccgcca
     9421 acaccataga gaaaactgcc ctcatatttg tcaccgattt ttccactatc ttcttggaga
     9481 aactccatat cttttttatt ttcggagact tttaactttg cttcattgat tcgaaccatg
     9541 agatccgaaa gatactcacc cattttatcc cgactggtca gagagatgag attttcaggt
     9601 tgaatttgaa agtcggctaa ctcctgggtc agacttttga tgttcttatc cactctctgt
     9661 ttttgatcat tgatgaattt ttcgacctca tttatttcat ccatctcgcg atcgcgtaag
     9721 tattccgaag aaagctctaa ggccgtattg gtcaagaaaa gcgaaagctc ttttgaaggc
     9781 gtggaaaaag agatgcgaat ttcataatcg gattccaatt tcgcctgaga ccaagagtcc
     9841 agttttacaa taaagttctg tcggctgctc tcatccttga aagcttgcat aagatatttg
     9901 tctttaatgg attgataagc caactgttca tcgacattta gaagtaaact tttaccacgc
     9961 tcgtcaagac gttgaagaag tttttcataa aactcacgag ttctcaaaac ttcgacctgt
    10021 tttccaattt gagtatttcc cttttttcca tcgaccactg actttgtcat tccaaaataa
    10081 gccattgaaa aagcctggag agagctgttt tgtgaatcat tcaccaacag ccggcctctg
    10141 ccgacatagg ggattttgaa gatgtatacg agaagggaga ccgtgaggaa aacaatgaag
    10201 attcccccaa aaagttttcc atgtctttta agaagaacta caaagtcacg gggttgaaaa
    10261 atactctcgg cttttctttc tgataaattt tccatagaaa gaccttatgc cataatcctt
    10321 tatcttcagg atagtgagca caaagccttt gaagaaagct tatttggtca gtcacacgct
    10381 cgtcacacga gagaatttta ttagaatttt gtactggcga tacgcggaaa cttaaccgga
    10441 cgaatcaccc tcccagcggc atctgttgtt tgagatgtat cctcggaagt taccggtgat
    10501 ttattatctt ctacaaatcc ctgggtcacc tgctgcaggt gattggcggt ctcgcaagct
    10561 tggcgaaaag catcgccaag tttcttcaaa gcttcttggt ccgctgtggg cacttcaaat
    10621 gctttcgtta aatccactgg aatctcgcga ctaagaggtg cttccatatc aatacgggat
    10681 tcttgaaaag aatccacctc atttgactca gcgtattctg ggggcgttgg ttgggtggag
    10741 gcttgggccc attcgggaag gctctcttca caaaacatca attgatcttt attcacctgg
    10801 gcgatcattt cgatttcgcc caaagaaaga tccacttgag aggcgatttc ggcggcactc
    10861 tttccctgat gagctaagcg ggctgctttg acatatttaa ttgtactctg ccgctccacg
    10921 atctcattgt gagggattcg atcttgaaat atttttgcca cttcaagact cttttgcata
    10981 ctttgttcaa tttttagaag ttgcttatca gaatgttgaa cctgctgttg gatctgacgt
    11041 actttttgtt ctaacaaaga ggtgagttgc tggacctgta cttcggtacg atccgaaaga
    11101 tcttccagaa cagcgatttt actttgcaga agttgtaggc ctttactcaa gcgtggatca
    11161 tcttttggcg gacgttgaaa tcggatccaa acacccacag agcctgcaat taaaattaaa
    11221 tccaccaata cttgaagtaa tagccaaaaa ctcacaggcc ctccatcgtc ctttatacct
    11281 ccagcttagt agaaagaacc taggccccac aacggaccaa ggtctcagtt tgaagcaaaa
    11341 gctttcgtag gagtcctgtt tatcactcat tttgaatttt ttcggtctca gcacctttgt
    11401 ctccaaacga cactatgtta agattttctt gggggattaa tccatgacgt cttctatttc
    11461 gattttgtct atttggactt tgaaaagact gactattctt gttttgtccg cagttctttt
    11521 accctcttgc ggaaacgttc gctttaatgc ggacgcccct gttcaagcag cgagtcctgg
    11581 aaccactggt tctggaactg ttaacgacaa tggtggaact gcaccgggga cctccccgac
    11641 tcctactccc agcgctcctc gcatcgtcac ttactcgagt gttgttccgc ccaatgctaa
    11701 taaagtagat atccttttga ttattgatga ttccggctct atgaaaactg atcagttgaa
    11761 attggcaaat aagctttcgg gatttgcaag ccagcttgaa agttcttctg cacccatgga
    11821 ttggcaaatg tgcgtcactg ttacgcgcga tcagctcatc agcggtaaat attattgggg
    11881 cgcctctgtt aactgggtgg gttattcacc agcttcagga cctcaatatg ttcttaaaaa
    11941 aggaaccacg gggttgaatg acatcttcac caagaccatc aatgctattg gtgctggagt
    12001 cccagggtcc ggagatgagc gagctattaa agcggcttat caccatttct ataatggaga
    12061 accaggcgtt gccggcacca gcgggtgcta tcgtaaagat gccgccgtcg ctgtgatagt
    12121 tatctctgat gaaaatgagc gtagtgttgg cggcgattgt acaagcatta aatcgaatat
    12181 gaacgaagat cccgcatcct gccttgcttt agaagccgag gatcagccat caacgttgtt
    12241 gactcaagcg aaaagtatct ttggtcctaa cgctcgtttt agtttcaact ccattgtggt
    12301 gggagatgcc gtgtgtgaaa gcacccaaga tcaaactgcc gatgaaaaag gccagagatc
    12361 ccctagccat caaggtttga aatacatcga aatatccaaa ttaaccaatg gcggagtgag
    12421 cagcatctgc gaaaatgatt tctcaaccaa cttgaatatc tttaaagaca agattttgaa
    12481 ttctctttct tctttgactc tggaatgttc acccatcgca ggcagtttga gcgtctcgat
    12541 taataatgta cccacaacag attatgtttt aatgggcgct aatttgaaat tcaacaatgc
    12601 cgttgttgaa ggaaaaaccg tttcgatgac ttacaaatgt agtcagtagt cataaattgc
    12661 acgtctgcaa agaggtttta cagtcgaaaa aagagtgtac gacctcctcc tcaaatctca
    12721 aattttgtac gttctaaagt aagactgagg ctctatacct ttatctattt atattttgct
    12781 atgctaattt gttaaaaggg ggaatggcca tgcatcagaa tttatca
//
LOCUS       DATBTE010000478         2672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_750829, whole genome shotgun sequence.
ACCESSION   DATBTE010000478 DATBTE010000000
VERSION     DATBTE010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750829"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..1047
                     /locus_tag="VIG33_18185"
     CDS             25..1047
                     /locus_tag="VIG33_18185"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY8272828.1"
                     /translation="MYKHLISNKLLKNIKSISIKISELEKRNFSEPVLAKYELSANLL
                     SAYSSTSIEGNPLALTEVKKILKSRPSQVRKSEQEVLNYNDCLVWLNSGIKKNNIHFN
                     YEIVLKIHKIVMKDLISKGSAGKIRNEPVFVNDPKLRKTIYWPPDHQEVPTLLKELFA
                     FVKKNEEEMDPLILAGIFHKQFVVIHPFIDGNGRSVRLATKVLLAKLGINTFNLFSFE
                     NYYNRNVSRYFANVGVMGNYYDVVEKLDFTQWLEYFSDGILDELSRVQKDLENEIKTP
                     TDLITSHQELILNYIRKYGFIRDKDYAKLTDRAKATRTLDFKKLIEQGYIERHGKGPT
                     VFYKFK"
     gene            complement(1087..2166)
                     /locus_tag="VIG33_18190"
     CDS             complement(1087..2166)
                     /locus_tag="VIG33_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272829.1"
                     /translation="MINSAIACSLIFSGLAFAQPLPLHFKQVDLSENISQLLRSNADH
                     CWTYNKDHRVLKDLNYYLDAEGVISGDPHLGNTTVIPVRTKEGVEELRLVNVDFDDGG
                     HGPFALEFARFATVAKASSKEIKIKDMSKAYLKGLNGQQMPMPKRIAQALSLDLKKYE
                     SLRAEYVEDKMKEGHFKYKKGEIEKWNGHPNSLEVAPFFRGSKVIDVAQRPAERGGSL
                     ASLRLWVLLLDEAGAYRIKELKQTQDTALSEYSPQASLQERIEALHKTYWEDLDQSVY
                     DVADIQGISFWVREKKVEILSYKDKQEEEEGHIYLANLMGLTQAKQTAGPAYATKISE
                     DPDRFKKAVKEYVHTYLGIAKDTMD"
     gene            complement(2170..>2672)
                     /locus_tag="VIG33_18195"
     CDS             complement(2170..>2672)
                     /locus_tag="VIG33_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272830.1"
                     /translation="QNVADMIVMDTMLNQQDRFGNVHTTNSYIYLDSSEGVSVKLKNK
                     MTETEVRSTGAVLVKNMMLKDNDCGVAKENAAKKAGLVQGLSHISPETYARLLKLQVE
                     VSQETTKSFFKDETLMTDVDYASMKQNLSEIVQTLKKACREGRLQLDLDLNGHFANKP
                     LNQSCE"
BASE COUNT          804 a          601 c          479 g          788 t
ORIGIN      
        1 ctaataatag actaataata gactatgtat aaacacctga tctcgaataa attgttaaaa
       61 aatattaaat caatatcgat aaaaattagc gaacttgaaa aacgaaattt ttctgaacct
      121 gtcttagcga aatatgaact cagcgcaaat ttattatcgg cctattcgtc tacgagcatt
      181 gaagggaatc cccttgcact gacggaagta aaaaaaatat taaaaagtcg cccatcacag
      241 gtccgcaaaa gcgaacaaga agttttaaac tacaatgact gtcttgtttg gttaaactcc
      301 ggtatcaaga aaaacaatat tcatttcaat tatgaaatcg tcttaaaaat tcataaaatc
      361 gttatgaagg atctaatttc taaaggctcc gccggaaaga ttcgaaatga acctgtcttc
      421 gtaaacgatc caaagttaag aaaaactatc tattggcccc ccgatcatca agaggtcccc
      481 actcttctaa aagaactgtt tgcatttgtt aaaaaaaatg aagaagagat ggatcccctt
      541 atacttgcag gtatttttca caagcagttt gttgttattc atccttttat tgatggaaat
      601 ggccgctccg ttcgcttagc aaccaaagta cttttagcca aacttggcat caatacattc
      661 aatctcttta gttttgaaaa ctattacaac cgaaatgtgt cgagatattt tgccaacgtc
      721 ggagtcatgg gaaattacta tgacgttgtt gaaaaactcg attttacaca atggctagaa
      781 tatttttcag atggaattct ggatgaatta tcgcgggtcc aaaaggatct cgaaaacgaa
      841 ataaaaacac caacagatct tataacttcg caccaagaat tgattttaaa ttatatcaga
      901 aagtatggtt tcattcgaga caaagattat gcaaaactaa cagaccgagc caaagccacc
      961 agaactctag attttaaaaa acttatcgaa caaggttata ttgaacgcca tggaaaagga
     1021 ccgacagttt tttataaatt taaatagtct aaacttcgtc tgcttggacc cgagctggac
     1081 tgctccttaa tccatagtat ctttagctat gcccaagtaa gtatgaacat attctttcac
     1141 ggcctttttg aagcgatccg gatcttcaga gatcttggtc gcgtaggctg gacccgccgt
     1201 ctgtttggct tgagtcagac ccattagatt cgcaagataa atatgacctt cctcttcctc
     1261 ttgcttgtct ttataactca agatttcgac ttttttctca cgaacccaga aggaaatccc
     1321 ttgaatatcc gccacgtcat aaacgctttg atccaaatct tcccaataag tcttatgcaa
     1381 agcctctata cgttcctgga ggctcgcttg cggagagtat tccgaaagag cggtgtcttg
     1441 ggtttgcttg agttctttga tgcgataggc cccagcttca tcgagcaata agacccagag
     1501 gcgaagactg gctaggctgc caccacgctc ggcaggccgt tgggccacgt caatcacttt
     1561 agaacctctg aagaaagggg ccacttcaag gctattgggg tgcccattcc acttctcgat
     1621 ttcgcctttt ttatacttga aatgtccctc cttcatttta tcttcgacat actctgcacg
     1681 cagggactca tactttttaa gatctaagct taatgcctgc gcaattcttt ttggcattgg
     1741 catctgttgt ccgttgagcc cctttaagta agctttagac atatccttga ttttgatttc
     1801 ttttgaagaa gctttggcca cagtcgcaaa acgagcgaat tccagcgcaa agggcccatg
     1861 ccccccatca tcaaaatcca catttaccaa acgaagttct tcgacgcctt ctttggtcct
     1921 cactggaatc acagtggtat ttcccaaatg aggatctcca ctgatgacac cttcagcatc
     1981 taggtaataa ttcaaatctt taagaacccg gtggtcctta ttgtaagtcc aacagtggtc
     2041 cgcattgctg cgaagaagct gagaaatatt ttctgataaa tccacttgtt taaaatgcaa
     2101 gggcaatggc tgagcaaaag ccaacccgct aaatattaaa ctgcaagcaa tagctgaatt
     2161 tatcataaat cactcacaac tttgattcaa aggtttattg gcaaaatgcc cattcaaatc
     2221 tagatctaat tgcaaacgcc cttcacgaca agcctttttc agagtttgta caatttcgct
     2281 cagattttgt ttcatggaag cataatcaac atccgtcatc aaagtctcgt ccttaaagaa
     2341 actcttcgtc gtctcctgag atacttcgac ttgcaatttc aagagccgag cataggtctc
     2401 tggactgata tgagaaagac cctgcacaag gcccgctttt ttcgccgcgt tctcttttgc
     2461 aaccccgcag tcgttatctt tgagcatcat gttcttaacc agcaccgcac ccgtactgcg
     2521 aacttcagtc tccgtcattt tatttttcaa ttttactgat actccctctg agctatctaa
     2581 atagatataa gaattcgtcg tgtgaacgtt gccgaaacga tcttgttgat tcagcatggt
     2641 atccataaca atcatatcag ccacgttctg ca
//
LOCUS       DATBTE010000479         4523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1160208, whole genome shotgun sequence.
ACCESSION   DATBTE010000479 DATBTE010000000
VERSION     DATBTE010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160208"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(310..789)
                     /locus_tag="VIG33_18200"
     CDS             complement(310..789)
                     /locus_tag="VIG33_18200"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY8272831.1"
                     /translation="MLKVYACVWSDKSATEMVKFYKSVFKGTKVGKYAYWGKNPMGVK
                     EGSVLTAHITILGQKLMLLNGFMKMPFNESISFVVPCKTQREIDTYWKKLTAGGGKPV
                     ECGWVIDKFGVRWQITPADFDKWNTTKNTKKKEAMMAAMWKMKKLDIKKLKAAFDNA"
     gene            complement(1142..2587)
                     /locus_tag="VIG33_18205"
     CDS             complement(1142..2587)
                     /locus_tag="VIG33_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HEY8272832.1"
                     /translation="MQNIGRNDACPCGSGKKYKKCCLPNQQLASDDLAWQRIHNMHHK
                     LVSKLMEFTAKTYRATGYDEAWDEFNLWNNVESFDPESPLHPMFGPWMFYHWSPDPAE
                     TKLSEEIPMDMTPAEAFLEKFGNKLDDLEIEDLEENLRRPFSFYDVLECKPGEWVKVQ
                     DLLTEEYFIVIEKMGSQSMRAGDLLFGKVVTVRNVSMFDGLAPILFPPQHKITVIEER
                     NDYKNDEGQITAEGLRESDLELLELFWDLYEAVTNPPAPILTNTDGHLMIPHKMTFSI
                     DSIEDAFEALHDLDFNDSKEEILENADYDKEGLLKVVEFPWLMKGNKQNLHWDNTVLG
                     HIRLESNKMIVDVNSKERSKKFLTVLKKRMPKGWTLKTTVLEDLQAKVREQGKQSRSL
                     GDDHEIKELNERPEVKEYLAKMNEGHWKAWPMTPLPALKGKTPVEAVKTKDGRAMVDA
                     LLTQFERSAERTPMPGQTIETFQILRERLGL"
     gene            complement(2663..3811)
                     /locus_tag="VIG33_18210"
     CDS             complement(2663..3811)
                     /locus_tag="VIG33_18210"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY8272833.1"
                     /translation="MGRSGQYVQQLKGYKAFIPAPLPPDPGLKQDDEFNYLLSNASLA
                     LGKLSGLASVISDPDLFVYLYVRKEALLSSQIEGTQCSLEDVLSPGAEEERSKHDDIE
                     EVSNYVKAMNEGLKRLSELPVCTRLIKEIHSTLMSGVRDANKTPGEFRQSQNWIGRPD
                     ANLNTAEFVPPPFEEVDRLMSDLEKFIHADDKIPYLVKAALIHAQFETIHPFLDGNGR
                     LGRLLITYLLCFWKVLDRPLLYLSYYFKAYRSEYYTKLMDVRVKGDWESWIKFFLKAV
                     SESADMANSAALEIHRLHQVDKERLQQSKPTTYTIQVFNEFCHDPILTGAALIERHSS
                     TKPKVNRAFQHLLDLGIIREISGKQRNRKYAYYKYLQILVRDTNTKVG"
     gene            complement(4290..>4523)
                     /locus_tag="VIG33_18215"
     CDS             complement(4290..>4523)
                     /locus_tag="VIG33_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272834.1"
                     /translation="FTYQEDFRLARGLSVMLEKLPEKNGKIIIEQRYPRSELFGMPAL
                     QNILTTFRCIYLELYWNSSKKLTLIDTIKGLIC"
BASE COUNT         1203 a         1045 c          898 g         1377 t
ORIGIN      
        1 tacttgccca aagatattac aaagactgat tctgtccgta catgcacatt taaaggtgtc
       61 gttcgatagt tcttccgaag catggtggtg gcaactaatg ctacagacac aagaagtgca
      121 aacttgaact tcaacaccta tactttgcaa aaccgaccaa gaaatcattc gaattatcgc
      181 ttgctgaaaa tcaactgacg gctcttgaaa atgaacgcat cgctttgctg agtgagttaa
      241 aaaatctgcg agcccaaaga gataaacaga aacctaggga tgaagtcagt tataagcctt
      301 tgcttcggat taagcgttgt cgaaggccgc tttcagtttc ttgatgtcga gcttcttcat
      361 cttccacatt gcggccatca tagcttcttt tttcttggtg ttcttggtgg tgttccactt
      421 gtcgaagtcg gcgggagtga tctgccagcg aacaccaaat ttgtcgatca cccagccgca
      481 ttccacgggt ttgccgccac cggcggtcag ctttttccaa taagtgtcga tctcgcgttg
      541 ggttttgcag ggcacgacaa acgagatgga ttcattgaac ggcattttca tgaacccatt
      601 caaaagcatg agtttttgac ccaggatggt tatgtgcgcg gtgagtacgg agccttcctt
      661 cacgcccatg gggttcttgc cccagtaggc gtactttccc actttggtgc ctttgaatac
      721 ggacttgtaa aatttgacca tctcagtggc gcttttatcg gaccagacgc aggcgtagac
      781 tttcaacatg ggatctccta gcacgtagct tagctcggga attgggtttg gactacatgc
      841 tgtgcgcaac gtatgtgggt tcgcatagac aggagccctt atgagtttgt tttttgttat
      901 tcatcatatc ctcctaaatc agggcaattt tccaagttga acttactttt tttgttgcgt
      961 gaattcatac gcagggtcac tccactggcg tttgcggtag tagcttgaat tgcattgtgt
     1021 tctctagatc ggcgtatctt tgtagaaatt gaaaaatcac ttaaaaataa tatttttgtc
     1081 gccaggaatg gacaacacat tataggccgc gcctttgcaa ttacttgaaa aaaaaacaga
     1141 ctcataatcc aaggcgttca cgcagtatct ggaatgtttc aatcgtttgc cccggcattg
     1201 gcgtgcgctc cgcactgcgc tcaaactggg taagcagggc atcgaccatg gctcgaccat
     1261 cttttgtctt taccgcctca accggcgttt tacccttaag agcgggaagc ggtgtcattg
     1321 gccaggcttt ccaatgaccc tcattcattt tggccaaata ttcttttact tcaggacgtt
     1381 cattcagttc cttgatttca tgatcgtcac ctaagctccg tgactgcttt ccttgttcgc
     1441 gcacttttgc ctgcaaatct tcaagaactg ttgtcttcag cgtccagccc ttcggcattc
     1501 gcttttttaa gaccgttaaa aacttttttg atcgttcttt agagtttaca tctacgatca
     1561 ttttattgct ttctagacga atatgcccaa gtacagtgtt atcccagtgt aggttctgct
     1621 tgtttccctt cataagccag gggaactcaa caaccttgag cagaccctct ttgtcatagt
     1681 cagcattttc tagtatttct tccttagaat cgttgaaatc caggtcatgc aaagcctcaa
     1741 aagcatcttc aatactatcg atagagaagg tcatcttgtg tggaatcatt aaatgaccgt
     1801 ccgtgtttgt cagtattggc gctggggggt tggtaacagc ttcgtacaag tcccagaaca
     1861 attcgagcaa ctcgaggtcg gattctcgca aaccttcagc tgtaatttga ccttcatcgt
     1921 tcttgtaatc atttcgttcc tcaatcacag tgattttatg ctgcggcgga aatagaatgg
     1981 gagccagacc atcaaacatc gaaacatttc taaccgttac aacctttcca aacagaaggt
     2041 ccccagccct cattgattga gagcccattt tttcgataac tataaaatat tcttctgtta
     2101 agagatcctg aaccttgacc cattcaccag gtttgcactc aaggacgtcg taaaaactaa
     2161 acggtcgtcg caaattttct tcaagatcct caatctcaag atcatccaac ttgtttccaa
     2221 atttttctaa gaaggcttca gcaggagtca tatccattgg aatttcttcg ctcaacttgg
     2281 tttcggcagg gtccggactc caatgataaa acatccatgg accgaacatt gggtggagtg
     2341 gcgattcagg atcaaacgat tcgacattat tccaaaggtt aaattcatcc caagcctcat
     2401 cgtagccagt ggctctatag gttttggccg taaactccat aagtttgctg accaatttat
     2461 gatgcatatt gtgaatacgt tgccaggcta agtcatccga agccagttgc tgatttggaa
     2521 gacagcactt cttatacttt ttgccacttc cacatgggca ggcgtcattt ctacctatat
     2581 tttgcatctc atcctctcat ttcctagggc gatctggctt ccgaatccaa agagacgaac
     2641 agaagcctat gctttccgtt ttttaaccaa ctttagtatt ggtatctctc actagaatct
     2701 gtagatattt gtaataagcg tattttctat ttctctgctt gccgctgatt tccctgatga
     2761 ttcccagatc caaaagatgc tggaatgctc gatttacctt tggcttggta gaggaatgcc
     2821 gttcaatgag ggcagcacca gtcaggattg gatcatggca aaactcatta aagacttgaa
     2881 tcgtgtatgt tgtaggcttg ctttgctgta agcgttcttt gtctacttgg tgcaaccgat
     2941 ggatctccag cgccgcgctg tttgccatgt cagcagattc cgaaacggct ttaagaaaga
     3001 acttaatcca gctctcccaa tctcccttca cgcgaacatc cattaatttt gtgtagtact
     3061 ctgatctata ggctttaaaa tagtagctca gataaagtag tgggcgatcc aaaaccttcc
     3121 agaaacatag caagtacgta attaacaaac gccctagacg gccattcccg tctaaaaacg
     3181 ggtgaatagt ttcaaactgc gcgtgaatca gagcggcttt tactaagtaa ggaattttat
     3241 cgtcagcatg tatgaacttt tctaagtctg acattaatct atccacctct tcaaaagggg
     3301 gaggcacaaa ttctgctgta ttcaaatttg catctggacg accaatccag ttttgtgact
     3361 gtcgaaactc acctggtgtt ttgtttgcat ctcgcacacc agacatcaac gtcgaatgga
     3421 tctctttgat caagcgagtg caaactggca attcgcttaa acgcttgagc ccctcattca
     3481 ttgctttaac gtaattagaa acttcttcga tgtcatcgtg tttacttcgc tcttcttctg
     3541 ctcccggaga taagacatct tccaaggaac actgagttcc ctctatctgc gagctcagca
     3601 atgcttcttt tctcacatac aaataaacaa atagatcagg gtcagaaatg acagaggcca
     3661 atcccgacaa cttacctagc gcgaggcttg catttgacaa tagatagtta aactcatcgt
     3721 cttgttttaa gcccggatca gggggcaaag gtgccggtat aaatgcctta tagcccttta
     3781 gctgctggac gtattgaccg gatcttccca ttacttagct tctcctcaag taacaatata
     3841 tatcaaaatc gttaccaata tacactgtag gtaacgattt tcaattaaaa tgttaccaat
     3901 ttacaccata agtaacgcac ggccaggctt ttctcaattt gatccatcac gctcttccag
     3961 cacgtactgt ccagattcag gatggacagt actgtccggt tttagaattt gccagtgctg
     4021 ttaatcactc cgtgagcaca tccttggaaa ttcgacaatc tatttaactg aaaatgaaat
     4081 gtcacaggta caactggcaa aaaaacttaa agtggatccc tcacgtatta atgaaattgg
     4141 gatcaagctc tctcaattct aaaatgttag aaattttatt ttacttgaac tcgtcctcct
     4201 atctgtatcc atacaatatc ccactcgacc taacgtcgac aatttcaacg gcgttctcga
     4261 ataacttcac attttgcact tagaaagtct taacaaatca gccctttgat ggtgtcaatt
     4321 agcgttaatt tttttgatga attccaataa agctcaagat agatacatcg aaaagtggtt
     4381 aaaatgtttt gtaatgctgg cattccaaat aattctgacc gtggatacct ctgttctata
     4441 ataatttttc catttttttc tggtaacttt tccaacatta ctgaaagacc acgagcaagg
     4501 cgaaaatctt cctggtaggt aaa
//
LOCUS       DATBTE010000480         3409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_819655, whole genome shotgun sequence.
ACCESSION   DATBTE010000480 DATBTE010000000
VERSION     DATBTE010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819655"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(402..1823)
                     /gene="murF"
                     /locus_tag="VIG33_18220"
     CDS             complement(402..1823)
                     /gene="murF"
                     /locus_tag="VIG33_18220"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEY8272835.1"
                     /translation="MRMMELKDVLAATKGQVLCGLHQKFLGIGTDTRQSLEGQLFVAL
                     KGESFDAHHFLDKAVAQGAAGLLVHEASPLIETLKNKTTVILVKDTLKALQDLGNWAR
                     QNTKATIIGITGSNGKTTTKEFVAAVLEKFKTVHYSKGSFNNHWGVPFTLLQLDPKKE
                     VAVVEMGMNHPGEITELVNIAEPDVVCCTMVGRAHIEHFGTIEKIAEAKEEIYETSKP
                     TATRVYNLDNPLTRKMYEKAPKKFPQAKLVTFSSHDPKADVYLKIEAMELQSIKMSGH
                     IESKEGEVTVPVFGIQNLTNLMAAAALGIATGLEPEQVWQGLSQCKTIWGRNQLLELK
                     SGTQMIFDGYNANPDSMKALLDNVKVLSIKGRKIGVFGEMLEMGTLANSLHEELGELV
                     GLTGFDKVYFVGTSHESFEKGLKKAHYKNSLKSAKAFTDEMAQDFAQDLHAGDMAVVK
                     GSRGMKLERFVLPCAPKDFSLNK"
     gene            complement(1820..3304)
                     /locus_tag="VIG33_18225"
     CDS             complement(1820..3304)
                     /locus_tag="VIG33_18225"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY8272836.1"
                     /translation="MKLQHLFSIFPGIPENAFSHIDVSDICYDARKVTPGAVFVAIPG
                     TKADGHEFLGDAISKGAVALVVENQEKVPEGFSGFVLQVPNSRQVLDILASRYYGDPG
                     RDLFCVGITGTNGKTSITYMIECIFDCARMPMGVIGTVNHRLGHKVWPSEMTTPDPAN
                     LQKRLNEFREEGATAVAMEVSSHALKQKRVDSVPFNSVVFTNLTRDHLDYHGTMDDYF
                     KSKQRLFTDLLWKTSKNPCFAIINTDDKWGRKLHVADPAILWTYGHREGDFQYEIIKM
                     GFTSTRFKVQTPAGDAEILLPMSGTHNVMNALAAIAVAVSAGIALPVSVKALASFTGV
                     PGRLQAVANDKDLSVFVDYAHSPDALENVLTALLKVRESLHSNAQVWTVFGCGGDRDK
                     GKRPLMTQMALKYSDHVIITSDNPRTEDPQQIIRDMTEQISAAEQTKIQIEVQRREAI
                     HRALSQAKPGDVVLIAGKGHEEYQIIGEEKLPFSDLQVAKEFLK"
BASE COUNT          898 a          846 c          707 g          958 t
ORIGIN      
        1 tcattggact tctggttgta ctcaaacaga agtaaagtcg cgctacgagt ttcaaaatga
       61 ataataagcg ccttcgcgac ttatgatagc cgtatcttcg tctagtagaa aatttaagac
      121 ccttctataa aacggagagc caatttatcg ttaggttaac tgcagcagct aagcattaat
      181 aaaaatacca gcatcaatca ctttagaatg accttagata tccgaaaaga aattgttcgg
      241 tttcttttca tagagtgcct cctttgggga gtctctcttc cttaaaagcg gtctctctga
      301 ggccgctttt attttatgca aagataatgt ccctctgaaa atgacagaga acagtgccaa
      361 cttccattgc tgtccaatta tcggaacgta ctctcggaat tctatttatt caaactgaaa
      421 tctttgggtg cacaaggaag aacgaagcgt tccaacttca taccacggga ccccttcacc
      481 actgccatgt caccggcgtg aagatcttgt gcaaaatcct gtgccatttc atcggtaaaa
      541 gccttcgcac tcttcaaaga atttttatag tgagcctttt taagtccttt ttcaaaagac
      601 tcatggcttg tgccgacgaa ataaactttg tcaaagcctg tgaggccgac cagctccccg
      661 agttcttcat gcaatgagtt tgctaaagtg cccatctcta gcatctcacc aaagacaccg
      721 attttgcggc ctttgatact caaaactttt acgttatcca gtaaagcttt catcgaatct
      781 gggttggcat tatagccatc aaagatcatc tgtgtgcccg attttagttc gagcaattga
      841 ttacgacccc aaattgtttt gcactggctt agtccctgcc aaacttgctc cggctccaac
      901 cctgtagcga tgccgagggc ggcggcagcc atgagattcg tcagattttg aattccaaaa
      961 accggaactg tcacttcgcc ttctttactt tcaatatgac cactcatctt aatactttgc
     1021 agttccatcg cttcgatctt caaataaaca tccgcttttg ggtcatgaga agaaaaggtc
     1081 actagctttg cttgagggaa tttctttggt gctttttcat acattttacg ggtcagagga
     1141 ttatccaaat tataaaccct ggtggccgtt ggctttgacg tctcatagat ttcttctttg
     1201 gcttccgcga ttttttcaat ggtcccgaag tgctcaatat gagcgcgccc caccatcgtg
     1261 caacaaacca cgtcaggctc tgcgatattt accagctcgg taatttcgcc gggatggttc
     1321 attcccattt ccaccaccgc gacttctttt ttgggatcca gttgcaacaa agtgaaaggc
     1381 accccccaat gattattgaa actgcctttg ctataatgaa cggttttgaa cttttcaaga
     1441 accgccgcca cgaattcctt tgtcgtggtt ttaccattag agccggtgat cccgataatt
     1501 gtggccttgg tgttttggcg tgcccaattg ccgagatcct gcaaagcttt caaagtgtct
     1561 ttcactaaaa tcacagtggt tttatttttt aaagtctcaa tcagaggcga agcctcatgc
     1621 acaagaagcc ctgccgctcc ttgagctacg gctttatcaa gaaagtgatg agcatcaaaa
     1681 ctttctcctt tgagagcgac aaaaagttgt ccctctaaag attgacgagt gtcggtccca
     1741 atccccagga acttctggtg gagtccgcat aaaacctggc ccttggtggc cgctagaacg
     1801 tcttttaatt ccatcattct cattttaaaa actccttggc cacttgaaga tcgctaaaag
     1861 ggagtttttc ttcgccaatg atttgatatt cttcatgacc tttacctgcg ataagcacca
     1921 catcccctgg cttagcttgg ctcagagctc gatgaatagc ttctcgtctt tgcacttcga
     1981 tttgaatctt agtttgttca gccgcagaaa tttgttccgt catatcccga ataatttgct
     2041 gcggatcctc ggtgcgagga ttatccgagg tgataataac atgatctgaa tacttgagcg
     2101 ccatctgagt catcaaagga cgtttgcctt tgtcacgatc tccaccgcaa ccaaacacgg
     2161 tccaaacttg cgcattcgag tgcagggact cacgcacctt cagcaaagcc gtcagaacat
     2221 tttcaagcgc atccggtgaa tgcgcataat ccacgaacac tgaaagatcc ttatcattcg
     2281 cgacggcctg caggcgtcct ggaacgcccg tgaacgaggc cagagccttc acgctgacgg
     2341 gcaatgcaat gcccgccgac accgctacgg caatggccgc taaagcattc atcacgttgt
     2401 gggttccgga catcggcaat aatatctctg cgtcgccagc gggagtttga accttgaagc
     2461 gtgtggaggt aaaacccatc ttaataattt cgtactgaaa atccccttca cgatggccat
     2521 aggtccaaag gatggcggga tccgcaacgt gaagctttcg gccccattta tcatccgtat
     2581 taataatagc aaaacacgga ttcttgctgg ttttccaaag aagatcggtg aaaagtcttt
     2641 gcttggattt aaaatagtca tccatggttc cgtgatagtc caaatgatca cgggtcagat
     2701 tcgtaaatac caccgaatta aaaggcacgc tgtcaacgcg tttttgcttc agcgcgtggc
     2761 ttgaaacctc catggccact gccgtagcgc cctcttcgcg gaattcattc aatctttttt
     2821 gcaaattcgc cggatccgga gtggtcatct cggaaggcca aactttatgt cccaaacggt
     2881 gattcacggt accaatcacg cccataggca ttcttgcgca atcaaagatg cattcgatca
     2941 tataggtgat cgaagttttg ccattggtcc ctgtgatccc cacacagaaa agatcacggc
     3001 cgggatcccc ataatagcga ctggctaaaa tgtccagcac ttggcgggag ttaggaactt
     3061 gtaaaacaaa accagaaaat ccttcaggga ctttctcttg attttctacc actagagcca
     3121 cagctccttt ggaaatcgca tctcctagga actcatggcc gtcggctttc gttcctggaa
     3181 tggcaacgaa aacggctcca ggggtcactt tgcgggcatc ataacaaatg tcgctcacat
     3241 caatgtgcga aaacgcattt tcaggtatcc cgggaaatat tgaaaataaa tgctgcagct
     3301 tcatattgac ctttgatatt catgaacaca ttatcgtaaa atgagaaaac tcgcaaaagt
     3361 atattgcctc gatgaatatc ataatgacaa tgccccctgg gttattctt
//
LOCUS       DATBTE010000481         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_272240, whole genome shotgun sequence.
ACCESSION   DATBTE010000481 DATBTE010000000
VERSION     DATBTE010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272240"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VIG33_18230"
     CDS             complement(<1..1322)
                     /locus_tag="VIG33_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011165799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY8272837.1"
                     /translation="MMSLENGTNAAEDLSQIFRVAHSVKGGAAAVGLPDLCKFAHVME
                     DLLSVLRADPELVNSNVISLLLRSGDELKKRISSLQNGENSPWDPSALAQELVQTHEN
                     LTGTKIQHHVKKVEDPEEVKAEAPDDFFSEPPENHANLELMAELLGQMSPEERATSEQ
                     PSSQPVLRSATKGSQKPQNVVIKVDTGRVDSVLDAVGELVVLKNQLVDDDYIKGGGNI
                     RLGGIVDQLDKAVRELYEKTLSIRMTPLKSLFVKMQRIVRDVSLSLNKPVDLQLIGEE
                     TEVERTVFELLGDPLVHLVRNAMDHGVENSQIRAERNKPAMAKVMVSAKQSGGNVMID
                     IVDDGGGINREKVLKKAIEKNLISPGVDPNSLSDDQVFQFIFSAGFSTADKVSDLSGR
                     GVGLDVVRSNLERINGKINISSKLGQGTTFRLTIPLTTAITDGIVVAL"
     gene            1721..2212
                     /locus_tag="VIG33_18235"
     CDS             1721..2212
                     /locus_tag="VIG33_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015092466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY8272838.1"
                     /translation="MSALTKKAKPGQYLTFQLQNEQYGVPIETVREINRVVEITPVPR
                     TPEYVKGVMNLRGKIIPVVNLRIKFGMEPQEHTRDTCIIVLDTEIGQVGMIVDSVKEV
                     VDLNEGQIEPSPVLGNEAAMSFVRGMGKVDNRVIILVDIVSAFSHEQLTGMSGIADHQ
                     DVA"
     gene            complement(2331..>2621)
                     /locus_tag="VIG33_18240"
     CDS             complement(2331..>2621)
                     /locus_tag="VIG33_18240"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY8272839.1"
                     /translation="SIVVNPSCLNHLDLALLEECLTRTHNTFVSVEDHQIIGGLGQMV
                     CHALAEEGMHFKCHTLGVRGEFGQSAYTALDLYKKHGIDSEAIVDNAIELIG"
BASE COUNT          775 a          611 c          514 g          721 t
ORIGIN      
        1 tcaagagcca ccacgatgcc atcagtgatc gccgtggtaa gaggaatcgt cagccgaaaa
       61 gtggtcccct gcccgagctt tgaagaaatg tttattttac cgttaattct ttccaggttt
      121 gagcgaacca catccaagcc gacccccctt ccagaaagat cgctgacttt gtcggctgtt
      181 gaaaagcccg cagaaaaaat aaattggaaa acctgatcat cagataagga atttggatcc
      241 actccgggag atatcagatt tttctcaatc gcttttttaa gaactttttc gcgattgatg
      301 ccaccaccat catccacaat atctatcatg acatttccac cagattgctt tgcggaaacc
      361 atcactttgg ccatggctgg tttatttctt tcggcgcgaa tttgactatt ctccacgcca
      421 tgatccatcg catttctcac caagtgaacc aacggatccc ctagaagttc aaagactgtt
      481 ctttcaacct cagtttcctc gccaataagc tgcaaatcca ctggcttatt gagactcaag
      541 gaaacgtcac gaacaatcct ttgcatcttt acgaagaggg attttaaggg ggtcatgcgg
      601 atactcaaag ttttttcata gagttcgcgg acggccttat ctaattggtc cacgatacct
      661 ccaaggcgta tattgccccc acctttaata taatcatcat caaccaattg attttttaaa
      721 accactaatt ccccaacagc gtccaagacc gaatcaacac ggcccgtatc gaccttaatc
      781 acaacattct ggggtttttg tgaacctttt gtagccgatc tcaagacagg ctgagagctg
      841 ggctgttcag aagtagcacg ctcttcgggg gacatttgcc ccagaagttc tgccattaat
      901 tctaaatttg catgattttc ggggggctcc gagaaaaaat catctggggc ctctgctttt
      961 acttcttcag gatcctccac ttttttcacg tggtgttgaa ttttagttcc cgtcaaattt
     1021 tcatgagtct gcactaactc ctgcgcaaga gcgctcggat cccagggact attctctcca
     1081 ttttgcaaag aggaaatacg tttttttagc tcatcaccag agcgtaaaag taaagagatg
     1141 acgttcgagt tcaccaattc tggatctgcc cgcaacactg ataaaaggtc ttccatcaca
     1201 tgggcaaatt tacaaaggtc cggcagacct acagccgcag ctcccccttt gacggaatga
     1261 gcaacccgaa aaatttgcga aagatcctca gctgcgttcg tgccattctc taaactcatc
     1321 attgattcct catatgactc gagaagaaac gctgattcat ttaggaactc catttgcagt
     1381 tctacaaaga aagagttgtc atcgctcata aaacccctat tttaaaatct ctggttttta
     1441 tcggcaaatc gaagaggaaa ctgtctactt ggactcctca agttgcagaa actgtcccta
     1501 cggctagaac ttgcaaataa ggatccgttt tgcttaatca gaatattcag catacttcag
     1561 aaaaaatgtt atctgaatgg accaacaaac atgaatcgat gtcctaatat gagccatagg
     1621 attttgtgga tcaaaattca ctggttttta gctaaagtaa acggcccaac cgatcgaaaa
     1681 gagaaagtga cattttgaaa tctcttgaaa ggggcaatga atgagtgctt tgacaaaaaa
     1741 ggcgaaacca ggacaatacc tgacgtttca actccaaaat gaacaatacg gagttccaat
     1801 agaaactgtt cgtgaaatca acagagttgt tgaaatcact cctgtacctc gtacacctga
     1861 atacgttaaa ggcgtcatga accttcgggg gaaaatcatt cccgtagtta acttgcgtat
     1921 aaaattcgga atggaacctc aagagcacac tcgagatact tgtatcatcg ttttagatac
     1981 cgaaatcggc caggtaggaa tgatcgtaga ttccgtgaag gaagttgtag acctaaatga
     2041 agggcaaatt gaaccttcac cagttctggg caatgaagcg gccatgtcct tcgttcgcgg
     2101 tatgggtaaa gtagacaatc gcgtgatcat acttgtggac attgtttcag ccttctcgca
     2161 cgaacaattg accggtatgt ccggcatcgc cgatcatcag gatgtcgctt aatcaaaaag
     2221 taattttaaa ttaataaaaa aggatggggc tttgagacca tcctttttta ttcgattcat
     2281 ttcattaatt ctaatttttt aatatatatt ttgaaattcg gggaaagctc ctagccaata
     2341 agctcaatgg cattatcaac aatggcctca ctgtcaattc cgtgcttctt gtaaagatcc
     2401 aacgccgtat aagcactttg gccaaactca ccgcggacgc ctagagtatg acatttgaaa
     2461 tgcattcctt cttcagccaa agcatgacaa accatctgtc caagcccacc aatgatttga
     2521 tgatcctcaa cggaaacaaa agtgttgtga gttcttgtaa gacactcttc caacaaagct
     2581 aagtctagat ggttcaaaca cgaaggattc accacaatag a
//
LOCUS       DATBTE010000482         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_272361, whole genome shotgun sequence.
ACCESSION   DATBTE010000482 DATBTE010000000
VERSION     DATBTE010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272361"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..>1718
                     /locus_tag="VIG33_18245"
     CDS             73..>1718
                     /locus_tag="VIG33_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272840.1"
                     /translation="MLLSASDLKNLEKLKHELQNETQSAKLENLAAALLGRLLGLSIS
                     IAKSGFQHGGDAGPAGRQERRFRIECKKYSDKTSLSDRELLGEIDQALARDDALEAWI
                     LVATREVPEQLEQDLFQHGEKHGVPVVIIDWKNHELASLAALCAFDPDLVEAEFSKEA
                     GALARALQPISADAIAMLRRDLQPWCLGFETLRLRSHEKLDKIWSSPKTSNAELGQDA
                     AGGAQEKRVKRSAVHQKLNSWWQGEARNDAPAAVVGLDGVGKTWATMDWLVNNKNEQP
                     IILIVPSSATPQMLSSISEISVKEFLANCLYQVTGVRNSEHWLRRLENLLERPETEGP
                     VLTVFFDGMNQEPSVKWSLLMKVLQSEVFCGRVRVIVSTRNHHFEEKLANLRALIVQA
                     VTVVVDSYDRMPGGELDQMLAFEGLSQTDLHSGLIELARIPRLFKLVVRFRSTLVEAN
                     QVTVHRLLWEYGRDTLGERAETSFSERDWRHWLKEIAGKYRDGISLTSIKELSETTSR
                     PDLTEREVYARLSDIIDGPFAHFDSSGNLNLSDTIVEHALG"
BASE COUNT          479 a          367 c          430 g          442 t
ORIGIN      
        1 gattattcga ttaaggatat tgatgagagg gagattgttg aaatggctaa atttttattg
       61 gcgttgtaaa tcatgctcct aagtgcaagt gatttaaaaa atctggaaaa actcaagcac
      121 gaactacaaa acgaaactca atcggccaaa cttgaaaatc tcgcagcagc tctgcttggc
      181 cgacttctcg gtctatcaat ttctatagcg aaatctggat ttcaacatgg aggagacgcc
      241 ggacctgctg ggcggcaaga gcggcgattt cgcatcgagt gtaaaaaata cagtgataaa
      301 acttctctta gtgatcgcga attgcttggc gaaattgatc aagcgctggc gcgggatgat
      361 gcccttgaag cctggattct agtagcaact cgtgaagttc cagagcaact tgagcaggat
      421 ttatttcaac atggtgagaa acatggcgtt ccagtcgtaa ttattgattg gaaaaatcat
      481 gagctcgcct ctctcgcagc attatgcgct tttgatcctg atctggtgga agcagagttt
      541 tctaaagagg ccggtgcatt agcaagagcc ttgcagccta tctctgccga tgccatcgcg
      601 atgttgcgcc gtgaccttca gccttggtgc ctcggcttcg aaacattgag attacgttcg
      661 cacgaaaaac tcgacaagat ttggagctcg ccgaaaacat cgaatgcaga actcggacaa
      721 gatgcggcag gaggtgctca ggaaaaaaga gtaaaaagat cggctgttca tcagaagctt
      781 aattcttggt ggcaaggaga agcgcggaac gatgctcctg ctgccgtggt cggattggac
      841 ggagtcggaa agacctgggc aacgatggat tggctggtca acaacaaaaa cgaacagccc
      901 atcattttga ttgtgccgtc ctcagccact cctcaaatgc tttcgagcat ttccgaaatc
      961 agcgtaaaag aatttctcgc aaactgtctc tatcaggtca ccggtgtaag aaattcagag
     1021 cactggttgc gccgtctcga aaacctgctt gagcgacctg agaccgaagg gccggtgtta
     1081 accgtatttt tcgacggtat gaatcaggag ccttctgtga aatggtcgtt gctcatgaaa
     1141 gttctgcaaa gtgaagtttt ttgtggacgt gtgcgagtga tcgttagtac tcgcaaccat
     1201 cacttcgaag aaaaacttgc aaacttacgt gctctcattg tgcaggccgt aacggttgta
     1261 gtagacagct atgatcgtat gccaggaggt gagctcgatc agatgctcgc gttcgaaggt
     1321 ttatcccaaa ccgatttaca ctcaggacta attgaattgg ctcgcattcc acgcttattc
     1381 aaactggtcg ttcggtttcg cagcacgctt gtagaagcta atcaagtaac cgttcataga
     1441 ctgctttggg aatatggtcg cgatacattg ggagaacgtg ccgaaacatc attcagtgag
     1501 agagattggc ggcattggct aaaagaaatc gctggaaaat atcgtgacgg catttcttta
     1561 acatcaatta aggagctcag cgaaactaca agccgtcccg atttaaccga aagagaagtg
     1621 tatgcgagac tcagcgatat tattgatggc ccatttgcac acttcgattc atcgggaaat
     1681 ttaaacttat ccgatactat tgttgaacat gctcttgg
//
LOCUS       DATBTE010000483         2171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2048695, whole genome shotgun sequence.
ACCESSION   DATBTE010000483 DATBTE010000000
VERSION     DATBTE010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2048695"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..590)
                     /locus_tag="VIG33_18250"
     CDS             complement(162..590)
                     /locus_tag="VIG33_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272841.1"
                     /translation="MLFSDFIYHALLDGHILNFKYQRISACNFRTDGIHLDPTNRGVA
                     IFAKAFMGLPAQQAAKTTRPLMPAAAPAVPLLSLDTQQGVSNIILAMTRRDVGKACIF
                     TTQKTLRENTCAIASGDYSTPVSQRMLVVGTSQRFDDDRL"
     gene            610..1176
                     /locus_tag="VIG33_18255"
     CDS             610..1176
                     /locus_tag="VIG33_18255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272842.1"
                     /translation="MKDKENPVILVYRRTHTGDPNDGDGGGIFGFNDCMKSVRDWEYD
                     AVIGIGGSKPDRGYEKIKEKITWIGICPKKHLVTQEDKERMKVNNPEFEDFGGRMVTF
                     DRFLFLDKPVSDDCPELYKHMFEEGKIPHQAKNFDKYPMIYNELKAILKLADDAPQSP
                     ARDILGTANPVGPTYSSSKGTQKNKGCA"
     gene            1173..2042
                     /locus_tag="VIG33_18260"
     CDS             1173..2042
                     /locus_tag="VIG33_18260"
                     /inference="COORDINATES: protein motif:HMM:NF037411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272843.1"
                     /translation="MKIIFSRKGFDSSAGGSPSLIFPDRTLFSIPIPSSKDDYCYSEL
                     SFQYQGDSIQSILNEITGKRIRHNKQRTDCDFSLTQQRCHYDPMPIHQANRLVLGQAG
                     NAEIHLQNQRVGIGDIFLFYGWFRQVEKKDGRWQYLPSSRDIHLIWSWMTIGETFDLG
                     TQSQREQAKANYQFLHRHPHLADWREAPNRIYISKEGGLLSYSTSRCLTECMEYRGRS
                     TWRLPACFNQPQAFTFLKNFILDGDDVIISYQGYGQEFVLDLHMVSSENDKTKILAYM
                     NQNILGGSEQSVP"
     gene            complement(2064..>2171)
                     /locus_tag="VIG33_18265"
     CDS             complement(2064..>2171)
                     /locus_tag="VIG33_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY8272844.1"
                     /translation="GKTKMSALGAAMRKLVHLCFGVLKTRQPYQSNYAI"
BASE COUNT          670 a          416 c          497 g          588 t
ORIGIN      
        1 agtttttatt ttttgttttt cttgatccat tttcgacaca ctctctaatt tcagattatt
       61 ttagattgtg tgtccggttt agtgtagcca cttcaatagg gcagaaaacc tgacagagcc
      121 gacatccaaa aataaataaa aaattgaaac gttagaacgt gtcaaagccg gtcatcatca
      181 aatctctgcg acgttccaac taccagcata cgctgcgaga caggagtcga gtagtcccca
      241 gaagcgatag cgcatgtgtt ttcacgaagc gttttttgcg tagtgaaaat gcaggctttc
      301 ccgacatcgc gtcgagtcat tgcgagaatg atgttggata ccccttgttg ggtatccaac
      361 gaaagaagcg ggacggctgg ggccgccgca ggcataagcg gtcgcgtagt ttttgccgct
      421 tgttgggcag ggaggcccat aaaagctttc gcaaaaatag cgactcctcg gttggttgga
      481 tcgagatgaa ttccatcagt gcgaaagttg caagctgaaa tacgttgata tttgaagttt
      541 aaaatatgcc catccaacaa ggcgtgataa ataaaatcag aaaataacaa taaaagatgg
      601 agaaataaat tgaaagataa agaaaatcca gtgatattgg tataccgtag aacccataca
      661 ggcgatccaa atgatggtga tggtggtggt atttttggat ttaacgattg catgaagagc
      721 gttcgagatt gggaatacga tgcggtcata ggcattggag gatctaagcc tgatcgtggt
      781 tatgaaaaaa taaaagaaaa aatcacttgg ataggaatat gccctaagaa gcatctcgta
      841 acacaagaag ataaggaaag gatgaaggtg aataaccctg aatttgaaga tttcggaggc
      901 cgtatggtga ctttcgatag attccttttt ctggataaac cagttagcga cgattgccct
      961 gaactctaca aacacatgtt tgaagaaggt aaaatccccc accaagccaa aaattttgac
     1021 aagtatccaa tgatttataa cgaacttaag gcaatattga aattggcaga tgacgctcca
     1081 caatctccag cgagagatat tttgggcaca gcgaaccccg tgggtccgac gtattcatca
     1141 tcaaaaggaa cccagaagaa taagggttgt gcatgaaaat cattttcagc agaaaaggat
     1201 ttgatagctc cgcaggtggc tcacctagtc tgatttttcc tgacaggact ttgttctcta
     1261 taccaatccc cagcagtaag gatgattatt gttattcaga actttctttt caataccaag
     1321 gtgattcgat ccaatcaata ttgaatgaaa tcacggggaa acggataaga cataataaac
     1381 aacgtactga ttgcgacttt tctctcacac aacagcgctg tcactatgac ccgatgccga
     1441 tacatcaggc gaatcggttg gtgcttggtc aagctgggaa tgccgaaatt catttgcaaa
     1501 accagcgtgt cggcataggg gacatctttc tattctacgg atggtttcgg caggttgaga
     1561 aaaaagatgg gcgttggcaa tatttaccaa gttcgagaga cattcattta atttggagtt
     1621 ggatgacgat cggtgagaca tttgatttag gaacacaaag tcaacgtgaa caagccaaag
     1681 ccaactacca gtttttgcat cggcaccctc atttagctga ttggcgggaa gcaccaaacc
     1741 ggatttatat atccaaggaa gggggcttat tgtcttattc cacttctcgc tgtttaaccg
     1801 aatgcatgga atatcgaggg cgttctactt ggagacttcc cgcctgtttt aatcaaccac
     1861 aagcatttac ttttcttaaa aattttattt tagatggtga cgatgtaatc atcagttatc
     1921 aaggatacgg tcaggagttt gtgttagatt tacacatggt ttcatctgag aatgataaaa
     1981 cgaaaatttt ggcctatatg aatcaaaaca ttcttggggg tagtgagcaa tcagtgccgt
     2041 agataccgtc ttgaaagtca actttaaatc gcataattgg attgataagg ttgacgagtt
     2101 ttcaagacgc caaagcataa atgcaccagc ttacgcatgg cagcccccag ggcggacatt
     2161 ttggttttac c
//
LOCUS       DATBTE010000484         1735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1912607, whole genome shotgun sequence.
ACCESSION   DATBTE010000484 DATBTE010000000
VERSION     DATBTE010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912607"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..439
                     /locus_tag="VIG33_18270"
     CDS             74..439
                     /locus_tag="VIG33_18270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272845.1"
                     /translation="MNKYEKLFQEIYEMTKGGDLKWKLISRNAHSDIIFNPSLVFRQF
                     ESRMVRDGDEYKVLFVEKKSAEPDYDYLEKYWPEVVVLCDGELIASLGDSVIERQNLI
                     SLMELIESKSDKAKKLFKE"
     gene            786..1469
                     /locus_tag="VIG33_18275"
     CDS             786..1469
                     /locus_tag="VIG33_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272846.1"
                     /translation="MNTDDSNTFKDTFTDERVNMIVDAVRTAVNNACDQYNPSIGSNA
                     TTFGVDVYNFGINELKKLVTKQPQIFRYLASGNLFRLFVEGFELAFHRVGYDVSEGIE
                     NSFPNNEGAVSRILFQQEEQLAFVGEGFDIQPSKKYVLGHFADPAGGLSGVYLCKPSK
                     KANAIAWDVTHELWKTDIENKEFKPTMKPKVAPVEKLGDIKLQPKEKLGEILLFPREE
                     KGTMKLDHE"
     gene            1462..>1735
                     /locus_tag="VIG33_18280"
     CDS             1462..>1735
                     /locus_tag="VIG33_18280"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY8272847.1"
                     /translation="MNDNIVPLGSKYIHTAAHLFQSKRLTLARELRGFTKAELADIVG
                     KTPSSISQFEDEDVNLKPDAATLGQLAMALSVPVQFFTRKMSSDLIH"
BASE COUNT          561 a          279 c          374 g          521 t
ORIGIN      
        1 atagaataac cgaggaaaaa atggatgaat taaaggaagt cataaatgat tggtcttcct
       61 actcggagga cccatgaaca agtacgaaaa gctgtttcag gaaatatacg aaatgacaaa
      121 gggtggagat ttgaagtgga agcttatttc gcgaaatgcc cattctgata ttatttttaa
      181 tccgagtctc gtatttcgtc agtttgagtc gagaatggtt agggacggtg atgaatataa
      241 agttcttttt gtagaaaaaa aatcagccga gccagattat gactacttgg aaaaatattg
      301 gccagaggtg gttgttctgt gcgatggaga gttaattgcc tccttaggag actctgtcat
      361 tgaacgacag aatttgatta gcttgatgga gcttatcgaa tcaaaaagtg acaaagccaa
      421 gaaacttttt aaagaatagg taactccctg tacgtctaaa attatttcct ccccgctcgt
      481 gataaagtgt gaaagctcgt cgcctcagcc aattcttgtg cctaatatac ctggttgaaa
      541 atcctttagt ttgttcagaa gccatattgg cattgaaact tggactaaag accagcatgt
      601 gtttgagata taactaaaag tgcttaaact actggctaat tgatgttgct acgaatcaaa
      661 ttttaattaa ttttctgatg aaagtgaaaa ttttaataaa tttatttgaa atataatgac
      721 catctactta acatttaatc ataaattaag cagttgtctt aagtttggca gctaaggatg
      781 gttaaatgaa cacagatgat agtaacacat ttaaagacac gttcacagat gaacgagtca
      841 acatgattgt cgatgcagtg agaacggcgg ttaataatgc ctgcgatcag tacaatcctt
      901 caattggcag caatgccacg acttttggag tggatgttta taactttgga atcaatgaac
      961 tcaaaaagtt ggtaaccaaa cagccacaaa tatttaggta tctggctagc ggtaacttat
     1021 tcagactttt tgtagagggc tttgagttgg cgtttcatag agttggttat gacgtttctg
     1081 aaggcattga aaactctttc cctaataatg aaggggcggt ctccaggatt ctttttcagc
     1141 aagaggagca gttagccttt gttggtgagg ggttcgacat tcaaccttca aaaaaatatg
     1201 ttttggggca ttttgctgat cctgctggtg gtttgtcagg agtctatttg tgtaaaccaa
     1261 gtaaaaaagc aaatgctatc gcttgggatg ttacacacga actttggaaa actgatattg
     1321 aaaataaaga attcaaacca actatgaaac caaaagttgc tccggtggaa aaacttggtg
     1381 atattaaatt gcagccaaaa gaaaaacttg gtgaaattct tttgtttcca cgagaagaaa
     1441 aaggcacaat gaagttagat catgaatgac aatatagttc cattgggaag caaatatata
     1501 catacagctg ctcatctctt tcagtcgaag agacttactt tagctcgcga gttaagaggt
     1561 tttaccaaag ctgagcttgc agatattgtt gggaaaactc ctagctcgat aagtcagttt
     1621 gaggatgaag acgttaattt gaagcctgat gcagctacac ttggccagct agctatggcg
     1681 ctttctgttc ctgttcaatt tttcacgaga aaaatgagtt cagatttaat tcatt
//
LOCUS       DATBTE010000485         3318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_2254063, whole genome shotgun sequence.
ACCESSION   DATBTE010000485 DATBTE010000000
VERSION     DATBTE010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2254063"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="VIG33_18285"
     CDS             <1..1264
                     /locus_tag="VIG33_18285"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEY8272848.1"
                     /translation="MKMHSLVSWLSDDRIDKALKNTEFADSPSYQGYLKARETYLHRS
                     LEQSREDLVFIRGNAKIMASLKALVTHEGWVHLIGNMLIFALFAIFVEQRIGFFGLIL
                     LYCIGGLGSNYLQLPFLPMGVRLFGASGAVSAVIGAFAVYFWREKMRCLLSVGFVYNR
                     MILIPAWLYIGLFLLLSDVVGMTGVGSGVAHLAHFTGFLAGFIFAFMQMDLFPMKNNF
                     LFPAESKLYYDAKETQVLEEKMDLFRRIYRLNRESFYSFRALFIYFEKHSCLLANFTE
                     EDQTFLTDIVRSCILYPEQDEKYAIAREILEKIPLTWNLSSLDLRIGPEELIKRAEQF
                     RLEGDLIQTLRFYDMFFAKFAVHPQAQDINSEIMKIFDQVEKFDLEIKIQILEALLVY
                     NDNHPDTQFQTQIRQLIHQVHREEKNAS"
     gene            1254..3305
                     /locus_tag="VIG33_18290"
     CDS             1254..3305
                     /locus_tag="VIG33_18290"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY8272849.1"
                     /translation="MRASISSRFSAMNLDMIIFGLLWQSMNMLIAKIFPEIRAPLNSL
                     LLGSLTAFFYFIYPTKKTGQTVGKRLLSLKVVPVAGSQVALTWSQAILRESGGKLLGC
                     LPLGLGYIWAYTNSEHKAWHDYLSKTQVISLIWEEEKTLLQKLQHIMLGILSIPLGVA
                     LILGIFLYTSLPLDSIKEKIEVSGIQVGHLSGSLAGGLHFSEIKRQDKDQSFYLNSID
                     VKCNLSALVYDRVFFIEKLTAEEGHIEVPENFSWATIFVNLISLGPTRDQGQDSDLGI
                     SLGNFKMGKLHFKNIFFEHNKKVILQLQEFALKNLEMADKELFINEAQFQTGGLTMKA
                     QDFKSSFGRLEVAAVTGGLGSEFLPILKVPVDFHLKGVVGKTPKGTKIEGGMTLDKIK
                     FTYENESLTVNVDKLLLNEMFKTALPLEELNLKLTAQGEDLLKLQSSLNVEYGIKVCG
                     NEFQPTQEKELLVFARTDRQFSFKMTPKPVMDLGKVIFAKESTLDDLFFYEVHGRKQI
                     GSDFISPQEMLADLCYQQTFARLQPSQAEALKPLVKVLETPPSSVELIQEVTREAQNF
                     LRLGKYTEAKSLLEAIQISSTPLSAVVLGAFYNVKAWAYLKSGDPQEASQAFEMAFAA
                     RKDISDAEGMWRANEALKKDQEALKWLDYIKMTLQTNPELSGHLTPNLKKKIVEETSS
                     H"
BASE COUNT          911 a          626 c          755 g         1026 t
ORIGIN      
        1 catgaagatg cattcgcttg taagttggct ctccgatgat cgtattgata aggcactcaa
       61 gaacactgag tttgcggatt ctccgagtta tcagggttat ttaaaagctc gagaaactta
      121 tttacataga agtctcgaac aatccagaga agatctcgtt tttattcggg gcaatgccaa
      181 aatcatggcg tcattgaaag ctttggtgac ccatgaaggc tgggtgcacc ttatcggtaa
      241 tatgctcatc tttgcattat ttgcgatttt cgtagagcaa cgaattggtt tttttggact
      301 catccttttg tattgtattg gcggcttagg ctcgaattat ttgcaattgc catttctacc
      361 gatgggtgtt cggctcttcg gagcctcagg agctgtgagt gcagtgattg gtgcctttgc
      421 tgtttatttt tggcgcgaga aaatgcgttg tctgttgagt gttggttttg tttataatcg
      481 catgatttta attcctgcat ggctttacat tggccttttt ctccttctca gtgatgtggt
      541 tggcatgaca ggggtgggca gcggagtggc gcatttagcg catttcaccg gatttttggc
      601 aggttttatt tttgccttta tgcaaatgga tctttttccc atgaagaata attttctttt
      661 tcccgccgag agcaaacttt attatgacgc caaagaaact caggttttgg aagaaaaaat
      721 ggatctcttt cggcgtatct atagacttaa tagagaaagt ttttattctt ttcgggctct
      781 ctttatttac tttgaaaagc acagttgctt actagcgaat ttcaccgaag aagatcaaac
      841 attcctcact gatattgtcc gcagttgtat cttatatcca gagcaagacg aaaaatatgc
      901 gatcgctcgg gagattctgg agaaaattcc gttgacatgg aatttgtcgt ctttagattt
      961 aagaattggg cccgaagaac tcattaaaag agccgagcag ttccgacttg agggtgattt
     1021 aatccagact ttgcgtttct atgacatgtt ttttgcgaaa ttcgccgttc atccccaagc
     1081 tcaggacatt aattcagaga tcatgaagat ttttgatcaa gttgaaaaat ttgacttaga
     1141 gattaaaatt caaattttag aggccttgtt agtatataat gacaatcatc ccgacacgca
     1201 atttcaaacc cagattcgcc aattgattca tcaagttcat cgggaggaga agaatgcgag
     1261 ctagcatctc ctctcgtttt tccgcgatga atctggatat gattatcttt ggactgcttt
     1321 ggcagtccat gaatatgctt attgcaaaaa tattcccgga gataagggct ccgttaaatt
     1381 ctcttctttt ggggtctctg actgcgttct tttactttat ttatcccacg aaaaaaaccg
     1441 gtcaaactgt gggaaagaga cttctttctt tgaaagtggt tccagtggca gggagccagg
     1501 tggctttaac ttggagccaa gccattctgc gggagagtgg gggcaagctt ctgggctgtc
     1561 tccctttggg gttggggtat atctgggctt ataccaactc tgagcataag gcatggcatg
     1621 attacctgag taagacccag gtgatttctc ttatatggga agaagaaaaa acacttttac
     1681 agaaacttca gcatatcatg ctggggattc tttcgattcc cttgggtgtg gctttaatct
     1741 taggcatttt cctttataca tctttgcctt tagattcaat taaagaaaaa atcgaagtat
     1801 cagggattca agtggggcac cttagcggaa gcctggccgg gggactgcat ttttcagaaa
     1861 taaaacgtca agataaggat caaagctttt atttgaattc tatagatgta aaatgcaatt
     1921 tatccgcctt ggtttacgac agagtatttt ttattgaaaa gctgaccgcg gaagagggac
     1981 atattgaagt tccagaaaac ttttcttggg ccactatttt tgtgaatctt atttccctgg
     2041 gcccaacccg ggatcaaggt caagattcag atctaggaat cagtctagga aattttaaaa
     2101 tgggaaaact tcattttaaa aatattttct ttgaacataa taaaaaagtt atcctgcaac
     2161 tgcaagagtt cgccctgaag aatctggaaa tggccgacaa ggaactcttc attaacgagg
     2221 cgcagtttca aaccgggggg cttacgatga aagcccaaga cttcaaatct tcctttggtc
     2281 gtcttgaagt ggctgctgtt accggaggtc tcgggtcgga gtttttacct attctgaaag
     2341 taccggtgga ttttcatctg aagggagttg ttggtaaaac tcccaaaggc actaagattg
     2401 agggcggtat gacgcttgat aagatcaaat tcacttatga aaatgagagc ctcactgtga
     2461 atgtcgataa gttattattg aatgaaatgt ttaagaccgc tcttcccctg gaagaactca
     2521 atttaaaatt gacggcccag ggagaagatc ttttaaagct ccagtcttcc ttaaatgtgg
     2581 aatatggaat taaagtttgc ggcaatgagt ttcagcccac tcaggaaaaa gaactccttg
     2641 tttttgctcg gacggatcgt caattttcgt ttaagatgac tcccaagccg gtgatggatt
     2701 tagggaaggt gatttttgct aaggaatcta ccttagatga tctgtttttt tacgaagtgc
     2761 atggcagaaa acaaattgga tctgatttta tctctcctca agagatgttg gcggatcttt
     2821 gctaccagca gacttttgca aggcttcaac cgtcgcaggc tgaagcctta aaacctctgg
     2881 tcaaagtcct ggaaactcct ccatcctctg tcgaacttat tcaagaggtg actcgcgagg
     2941 cgcaaaattt tttgcgattg ggaaaatata ctgaagctaa gtctttgctt gaagcgattc
     3001 agatttcctc aacccctctt tccgcagttg tgttgggggc tttttataat gtgaaagctt
     3061 gggcttattt aaaatcaggt gatccccaag aagccagtca ggcctttgaa atggcttttg
     3121 cggcccgcaa agacatcagt gatgcggaag gtatgtggag agccaatgaa gctttgaaga
     3181 aggatcaaga agccctcaaa tggctggatt acatcaaaat gacattacaa acaaatccag
     3241 agttatcagg tcatctaact ccgaatctga aaaagaaaat agtcgaagaa acttcttcac
     3301 attaagaaga gtgacgcg
//
LOCUS       DATBTE010000486         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_136494, whole genome shotgun sequence.
ACCESSION   DATBTE010000486 DATBTE010000000
VERSION     DATBTE010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136494"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(538..>2082)
                     /locus_tag="VIG33_18295"
     CDS             complement(538..>2082)
                     /locus_tag="VIG33_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272850.1"
                     /translation="RYPYNADTKESFYQAIFKELGLPYSKSEDNNSITMHQLLRMMYV
                     DQMTSPDRLFKFDRFDSPNKRKAIGELLIGLSDFSLYEKRVSLQKLEATLDNRLKEIK
                     TIHTFLGGTIKTVDEIDSEIHEKQAEIDKLEEEIEDSFSNTGNVESEEEEAILHKLAA
                     DIKKAREEHTRINEKISRTSFEINDSQLFIASLNARIVALRETKNTINALSDIAFNHC
                     PACQVELVKKASGCSVCGSEKSEDENDIDPTFKVRKEIEFQIAESKKLIDIKQHRLGE
                     YRVNFNQICTRLGELERNLDALRKPQRNINIQLRLKLNEVGALKSQITALNNSKGEFA
                     KLYGLYDEREALQGQVNSLKSEIQRAEAKVEREITNKKLRLSALTLELIRADKNHEEI
                     FNEGQIVDFDFAEDRVTIDDRALFSASSMVYLKNSFRLAMLKASCEDASYLYPRFLLM
                     DNVEDKGMEEERSQLFQHEIIKMSEDLTTVHQIIFTTSMIAPKLNYSDYCVGDFYNEQ
                     NKTLKI"
BASE COUNT          558 a          471 c          377 g          676 t
ORIGIN      
        1 taacccacgc tctccccgtt tctgtcggct tggtgaaacc cgctcaccct tttcgatggc
       61 gcgagaggaa agtagcttta attaatcccc atcgtgtaga aaaagcggta tcgttcagcg
      121 gcaaagtcca gatacccggc aactgcgccc tgcgtcgcct aaagcgccta ctgttggtgc
      181 tctacctcct gcatgaccca caaggatgtg ggaaatgcag aaaaatgact ggatcatttc
      241 ctgtccctgc agtcgaatgc aaatgctccg gcaaaattac cacagcatca attttaaacg
      301 ggtgtcgcgt tttaaccaga tcaaaagcgt tacgtaaacc atcgatattt tccaccagcc
      361 gcctatttcc ttttcgttca gccaaggttt acggtgaaaa accatgtagc tcccggtctg
      421 taaatacgac ggtaattagc catttttcat tgccaaagtt gggatggtgt ttcacgcgaa
      481 cgatgcggtt cgtacctcac cgtatcctga cctgatacag ctaagtaagc aacaccatca
      541 tatttttaga gttttattct gctcgttgta gaaatcacca acacaataat cgctatagtt
      601 aagtttagga gcaatcattg atgttgtaaa aatgatctga tgaacagttg ttaagtcctc
      661 cgacatctta atgatctcat gttggaaaag ctgacttctt tcttcctcca tccctttgtc
      721 ttcaacgttg tccatcaaca agaatcgagg atatagatag ctggcatctt cgcaagatgc
      781 ttttagcata gccagcctaa atgaattttt aagataaacc attgaactcg ccgagaataa
      841 agctctatcg tcaatagtta ctctatcctc agcaaaatca aagtcaacta tttgcccttc
      901 gttaaagatt tcctcgtggt tcttatccgc acgaatgagc tcaagagtca gtgctgaaag
      961 tcgtaatttc ttatttgtta tctctctttc aactttagct tcggcccgtt ggatttcact
     1021 ttttagtgaa ttaacctggc cttgcagagc ttcacgttca tcatacaaac catacagctt
     1081 tgcaaactct cccttgctgt tattcaaagc agtgatctga ctttttagtg cgccaacttc
     1141 attgagtttc aatctcaatt gaatattgat attacgttgg ggctttctca atgcgtctaa
     1201 gttacgttca agctcaccta atcgtgtaca aatctgattg aaatttactc tgtactcgcc
     1261 aagcctatgc tgcttaatat caataagttt tttagattcg gctatctgaa actcaatctc
     1321 ttttcttact ttgaatgtcg gatcaatgtc attttcgtct tcagattttt cgctcccaca
     1381 aactgaacaa ccagaggctt tcttcactag ctcaacttga catgcagggc aatggttaaa
     1441 ggctatatca gatagcgcat taattgtgtt ttttgtttct cttaatgcaa cgattcgagc
     1501 attcaaagaa gcaataaaca attgggaatc gttaatctca aatgatgttc tggatatttt
     1561 ttcattaatc cttgtatgct cttcccttgc tttttttata tccgcagcaa gcttatggag
     1621 tatagcctct tcttcttcac tctcgacatt tccagtgttg ctgaaagagt cctcaatctc
     1681 ttcttccaat ttgtcaattt cagcttgctt ctcatgaatc tcactatcga tttcatcaac
     1741 agttttgatt gtgccgccta aaaaggtgtg aatggtttta atctctttca gtctattatc
     1801 taaagtggct tcgagctttt gcaaactgac gcgcttttca taaagcgaaa agtccgatag
     1861 accgatcaat aattcaccaa tggctttacg cttgttaggt gaatcgaaac ggtcaaactt
     1921 aaacaaccgg tcaggcgagg tcatttgatc gacatacatc attcgtaaca actgatgcat
     1981 tgttatgctg ttgttgtctt cagatttaga ataaggaagg cccagttcct taaatatagc
     2041 ttgataaaag ctttctttcg tatcggcatt gtaaggatat ct
//
LOCUS       DATBTE010000487         6405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1707504, whole genome shotgun sequence.
ACCESSION   DATBTE010000487 DATBTE010000000
VERSION     DATBTE010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 6405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6405
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1707504"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VIG33_18300"
     CDS             <1..462
                     /locus_tag="VIG33_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HEY8272851.1"
                     /translation="NEEILSKLAMELELSKFLYLGKGETLTGGAQKPRLLASAYEALV
                     GAMFLDGGYGVVREFARGDFAKLIKDLDPAQDFERDYKTRLQERIQKETKEAPVYELI
                     SEEGPPHDKAFTICIRVKEVNLAMGTGRSKKIAEQEAAKKALEILLKEKNS"
     gene            459..1373
                     /gene="era"
                     /locus_tag="VIG33_18305"
     CDS             459..1373
                     /gene="era"
                     /locus_tag="VIG33_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY8272852.1"
                     /translation="MSYKAGFLGLIGQPNAGKSTLMNYLVNEKVSIVTEKPQTTRRRV
                     LGVWSADEGQIVFVDAPGLINADKGLNGFLSKEAEDVIGQSDAILAIISLDEKSAEDA
                     EKVIKMVSSSHKPWAGVITKSDLVEKQHRIMILKKMIEDAGGKAFQVTAKDMDADPEN
                     REALLLEFLALLPESPAPLYDVELFTPHNTRDLVAEVIREKCFEFVHQEIPFSMAVRI
                     LKFDENATPCPKLYAEILVAKENHKAIVIGKGGANLKEIGTTARKEIEKLLGEKVFLD
                     LIVNFKADWFNNKRIMQELGYVREHNED"
     gene            1348..2721
                     /gene="der"
                     /locus_tag="VIG33_18310"
     CDS             1348..2721
                     /gene="der"
                     /locus_tag="VIG33_18310"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY8272853.1"
                     /translation="MSENTMKTDDVEVPAEVLMTDVPKVAIIGRPNVGKSTLFNIITE
                     TRKAVVKDQAGVTRDIIIEPVDIWGKQFDLIDTGGITEAGDLFSKLIKEQVSEFLLSV
                     DFIVAVMDGRAGLLPEDRDIIKIAKQMGKPFLLVINKVDKVAEEEVSKADFYEFGVDI
                     VAASFEQRRGLSDILEWVTKQIPDRNETIAEGVKIAIVGKPNVGKSSLSNAILGTNRM
                     LVSDIAGTTIDSVECPFYANNKKYILVDTAGLRRSSRREEDLEIISAFKSKEAIRRSE
                     IILLVVDGTMGPTEQDAKIMQAILEDHRAVILVANKSDLGQKEVPEYKKTFREQVART
                     FHFFEDVPVVFTSAKMGHGLNDLYEMVDKVSAQLTMRIPTSELNDFFFETIRKAPAPV
                     HGNNNVKFYYLTQTYQKPPAFIAFANHPEGVTTAYRRFLIKNIKAKWGLQGIPIRIFC
                     MKSRKGS"
     gene            2721..4103
                     /locus_tag="VIG33_18315"
     CDS             2721..4103
                     /locus_tag="VIG33_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEY8272854.1"
                     /translation="MPAKRGSWRTRFGFYLLAIGSACGLGNLWRFPYVVGENGGGAFV
                     LLYVFMALTVGLPLLIAELSLGKSTRQSVLMATQKLAKNTGRKSLIWAGRFSVLLSLV
                     VFSYYAVISGWVLHFFSRFLMEILMPAEAHQSAASLATLMQNGWLQWALASVHVLLTI
                     IVVAKGVHEGLEKFISHMMPLFVTLVFLLVFRSTSLPSESEVLRFLFYPDFSKLTYSS
                     LIHAMGHIFFTLSVGFGTLVTFGSYMREEDHVPTAGFRVTLVDTGISLVAVLLVFPIA
                     FQASNVPLTDPALLFETLPRFFLDFRGGLLFGLLFFSCLYLAALNASIGLMETIVSNL
                     VDSSQKKKINRSKASWITGAFALFLAMLPAFSSSVFRNVQIFGKGLMESMDSVLINWI
                     LPMIALGLMVSINWGMTEKEKETQFIAKDRFVSYSMYPHWKWVLRWYAPGFIILGMLL
                     QILGLVLKMN"
     gene            complement(4116..4904)
                     /locus_tag="VIG33_18320"
     CDS             complement(4116..4904)
                     /locus_tag="VIG33_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272855.1"
                     /translation="MKILLIALLGFCSLATPAYAQSYLSDSPIQPIGHPSDKHYLLAQ
                     TDPDEAYDPFSDYSEFDEASDEEADINFFRNGRFFTIGLAAGVRNFTGNFSKIYSSAP
                     TFGLYLSYFFDLRLALSLGFLTGDNGVNIETATTTYTGNVSFTTLNFDLKYYLNTQNV
                     TRGLADLNPYLIGGFAQFYRTYTLAGLEGYSRDATMGIELGVGLEVPLMRKKAFLGIQ
                     GMYHYVNFSDENGKFINDGTSSIPLVNTISGDIYNILVILGMNF"
     gene            complement(5059..5412)
                     /locus_tag="VIG33_18325"
     CDS             complement(5059..5412)
                     /locus_tag="VIG33_18325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272856.1"
                     /translation="MSKAILSACLMLISGTAFAGHLNCYNANYNISVKGYFDTQSNTS
                     SKLTVSIGWPELGQQEVFESPKVLAEGTHNNDSNVYSFLGVYSGGPFNVLITLDPSNG
                     SLDSVRAFNRALVCK"
     gene            complement(5593..6216)
                     /locus_tag="VIG33_18330"
     CDS             complement(5593..6216)
                     /locus_tag="VIG33_18330"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY8272857.1"
                     /translation="MASKKSSPPKNKKTKLKNKAQQLCFFNKKIPRNFGGLLLKGNAK
                     RSRPLSIKEPLHLVLKSTQAIGSRSMLHSTHAKKIDKIIRHHAKQCRIRLYHLVNVGN
                     HLHLVIKLDDRKLYSKFIRAITGLIARQVLKAERGPAKEKNNLNIENQKKCFWLARPF
                     TRLIAWGRDYNFITGYMKKNKDQAQRFFTPWGFDVLDPEFIQFLKTG"
BASE COUNT         1712 a         1225 c         1610 g         1858 t
ORIGIN      
        1 aatgaagaga ttctttcgaa gctggcaatg gagttggagt tgtcgaagtt tttatatctg
       61 ggaaaaggcg agacgcttac agggggagcg caaaaacccc ggcttttggc ttcggcctat
      121 gaggccttgg taggcgccat gtttttggat gggggctatg gggttgttcg cgaatttgcc
      181 cgtggcgact tcgcaaaact tatcaaagat ctagatccgg ctcaggattt tgagcgcgat
      241 tataaaaccc gtcttcaaga acgcatacaa aaagaaacca aagaggcccc ggtttatgaa
      301 ttgatcagcg aggaggggcc tccccatgat aaggctttca ccatttgcat tcgcgtgaaa
      361 gaagttaatt tagccatggg gaccggacgc agtaaaaaaa tagctgagca agaggccgct
      421 aaaaaagccc ttgagatttt attaaaggag aagaattcat gagttacaaa gcaggatttt
      481 tagggcttat cggccagccg aatgcgggta aaagcactct tatgaattat cttgtgaatg
      541 aaaaagtgtc catcgtcacg gaaaaaccac aaaccacccg ccgccgcgtt ttaggcgtgt
      601 ggagcgccga tgaggggcag attgtttttg tcgatgcccc aggtttaatc aatgcggata
      661 aggggctgaa tggcttttta tctaaggaag ctgaagacgt gatcggtcaa tccgatgcta
      721 ttttggccat tattagtttg gacgagaaaa gtgcggaaga cgccgaaaag gtgattaaaa
      781 tggtttcgag cagtcataag ccttgggccg gtgtcatcac aaaatccgat ctggtggaaa
      841 agcaacatcg aattatgatt cttaaaaaga tgatcgaaga cgcgggaggg aaagcttttc
      901 aagtgacggc caaggatatg gatgcggatc ctgaaaatcg cgaagccctt ttgttggagt
      961 ttttggcgct gcttcccgag agcccggctc cgctctatga tgtggagctt ttcactcccc
     1021 acaatactcg ggacctggtt gcggaagtca tccgtgaaaa gtgttttgag tttgtccacc
     1081 aagagatccc ttttagtatg gccgtgcgca ttttaaagtt tgatgaaaat gcgactcctt
     1141 gtccgaagct ctacgccgag atcctggtgg caaaagagaa tcataaagcc atcgtcattg
     1201 gtaagggggg ggcgaatctg aaagagattg gcaccaccgc ccgtaaagag attgaaaagc
     1261 tcttgggcga aaaagttttt ttagatctaa tagttaattt taaagccgat tggttcaata
     1321 acaagcgcat tatgcaggag ctgggatatg tccgagaaca caatgaagac tgatgatgtg
     1381 gaagttcctg ctgaagtcct aatgacggat gtgcctaagg tggccatcat tggtcggccc
     1441 aacgtgggaa agtcgacttt atttaatatc atcaccgaga cccgcaaagc ggtggtgaaa
     1501 gatcaagccg gagtcactcg cgatattatt attgagcccg tggatatttg gggcaagcaa
     1561 tttgatttga tcgatactgg cggtattacc gaagctggcg atttgttttc taaactgatc
     1621 aaagagcagg tttctgaatt cttgttgagt gtggatttta ttgtggcagt gatggatggc
     1681 cgggccggac ttttaccgga agatcgcgat attataaaaa tcgcgaagca aatgggaaag
     1741 ccctttttgc tggtgatcaa taaagtcgat aaagtcgctg aagaagaagt ttctaaggcg
     1801 gatttttatg agtttggtgt ggacatcgtg gcggcctcct ttgagcagcg ccgtggactt
     1861 tctgatatcc tggagtgggt caccaaacaa attccggatc gcaatgaaac catcgctgaa
     1921 ggggttaaga ttgcgattgt gggaaaacct aacgttggta agagctctct gagcaatgcc
     1981 atcttaggga cgaatcgcat gttggtttct gatatcgctg gcacgaccat tgattcggtg
     2041 gaatgtcctt tttatgccaa taataaaaag tatattctcg tggatacggc gggattgcgt
     2101 cgttcgtcgc gacgtgaaga agatctggaa attatttcgg cctttaagtc aaaagaggcg
     2161 attcgccgct ctgaaatcat tttgctggtg gtggatggaa ctatgggccc cacggagcaa
     2221 gatgcgaaga tcatgcaagc cattctcgag gatcatcgag cggtgattct ggtggcgaat
     2281 aaatcagacc ttggtcaaaa ggaagttcct gagtataaaa agactttccg cgaacaggtg
     2341 gccaggacat ttcacttctt tgaggatgtg ccggtggttt tcacgagtgc aaagatgggc
     2401 catggtttga atgacctcta tgagatggtc gataaagttt cggctcaact caccatgaga
     2461 atcccgactt ctgagttgaa tgatttcttc tttgagacta ttcgcaaggc cccggcgccg
     2521 gtgcatggca ataataacgt gaagttctac tacttgactc agacttacca gaaacccccc
     2581 gcgttcattg cctttgccaa tcatcctgag ggagtgacca cggcttatcg tcgatttttg
     2641 attaaaaata tcaaggcgaa atggggttta cagggaatcc ctattcggat tttttgtatg
     2701 aaaagtcgta agggttctta atgccggcca aacgaggatc gtggagaacg cgttttggat
     2761 tttatctttt ggccataggc tccgcctgtg gtctggggaa tctgtggcgc tttccttatg
     2821 tggtgggaga aaacggagga ggtgccttcg ttttgttgta tgtgtttatg gcgctcacgg
     2881 tggggctccc tttattgatt gcggaattaa gtctgggtaa gtccactcgt cagagtgtgc
     2941 tgatggcgac gcaaaaactt gcgaaaaata cgggccgcaa gagtcttata tgggcgggca
     3001 gattctccgt tcttttgagc cttgtcgttt tttcttatta tgccgtgatc agcggctggg
     3061 tgctgcattt tttcagccgg ttcttaatgg aaatcttgat gccggcagag gctcatcaat
     3121 cagcggcttc gttggcgact ttaatgcaaa atggctggtt gcagtgggcg ctggccagtg
     3181 ttcacgtttt gctcacgatc atcgtggtgg caaaaggggt tcacgaggga ctggagaaat
     3241 tcataagtca catgatgccc ttgtttgtga cgcttgtttt cttattggtt tttcgttcga
     3301 cgtccttgcc ttcagaatca gaggttttgc gttttctttt ttatcccgat ttttcaaagc
     3361 tgacgtattc ttctttgatt catgctatgg ggcatatttt ctttacactt tcagtgggct
     3421 ttggaacgct ggtgactttt ggaagttata tgcgcgaaga agaccatgtt cccacggcgg
     3481 gattccgggt gaccttggtg gacacaggga tttcgttggt ggcggtctta ttggtgtttc
     3541 ctatcgcctt tcaggcttcg aatgttcccc tgacggaccc cgctttgctg tttgaaacct
     3601 tgccccgttt ctttttggat tttcgcgggg gtctcctctt tggtctgtta ttttttagtt
     3661 gtctttattt ggcggcactc aatgcgagca tcggtttgat ggagaccatt gtctccaatt
     3721 tagtggacag cagtcagaag aagaaaatca atcgcagcaa agcctcatgg atcacaggag
     3781 cttttgcctt gtttttggca atgctgcccg ctttttccag ttctgttttt agaaatgtac
     3841 agatttttgg aaaaggcctg atggaatcaa tggactcagt tttaatcaat tggatcttgc
     3901 cgatgatcgc tctaggactg atggtgagta tcaattgggg aatgaccgag aaagaaaaag
     3961 aaactcagtt tatcgccaag gaccgatttg tgagctattc aatgtatcct cattggaaat
     4021 gggtgcttcg ttggtatgct ccggggttta ttattttggg catgctttta caaattctag
     4081 gtctcgtttt aaagatgaat tagaaaaaaa atgaattaga aattcattcc caagataacc
     4141 aagatattat agatatcacc cgatatggta ttaacaaggg gaatggagct tgtgccatca
     4201 ttgatgaatt tgccgttttc atcgctaaag ttcacgtagt gatacattcc ttgaattccg
     4261 agaaaagctt tttttcgcat tagaggaacc tcgagtccca ctccaagttc aatgcccatg
     4321 gtggcatctc ggctatagcc ttcaagtccc gccaaagtat aagtgcgata aaattgcgca
     4381 aagccgccaa tcagataagg attcaaatcc gcaagaccac gggtcacatt ttgtgtattc
     4441 aagtaatact tgaggtcaaa gttcagcgtc gtgaaagaga cattcccggt ataagtggtc
     4501 gtggctgttt caatattgac gccgttgtca cctgtaagaa agcccaagga taaggctaaa
     4561 cgcagatcaa agaagtagct taagtaaagc ccaaaagtgg gggcactaga atagattttt
     4621 gaaaaattgc cggtgaagtt tcgtactcca gcggcaaggc caatggtaaa aaaacgacca
     4681 tttcgaaaga aatttatatc ggcttcctca tcggaagctt catcgaattc actgtagtcg
     4741 ctaaagggat cgtaggcctc atcagggtct gtttgcgcta agagataatg tttatcggat
     4801 ggatggccta tgggttgtat gggcgaatcg ctcaaataac tttgagcata agcaggagtt
     4861 gccaatgagc agaagcccag taatgcaatc aatagaattt tcacggtatc cccctgcttt
     4921 tattatcgcg aaaaatcagg aaaaactgaa ggtgggtccc actttgagac ggtaaaagtc
     4981 ttaaagtgga gcaagaatac cgctctgggt ggggagattt gactcgaaga agttcaaagt
     5041 tagggccaaa ttccctaatt acttacaaac tagggctcta ttaaaagctc tgacagagtc
     5101 tagagagcca ttggatggat caagtgtaat taacacattg aaggggccac ccgaataaac
     5161 acccaaaaat gaataaacgt ttgaatcgtt attatgagtt ccttcagcta atacttttgg
     5221 agactcaaac acttcctgct ggcctagttc tggccaacct atagaaacag tcagttttga
     5281 agatgtatta gattgagtat caaaataacc tttaacagaa atattataat ttgcattgta
     5341 acaatttaaa tgacctgcaa aggcggttcc tgaaatcagc attaaacaag ccgacaaaat
     5401 agctttactc ataaaaacct ccattagcgc tatcattagt aaatacctta tgtaagttca
     5461 atagtttaat taggctttga aaaaaatatc tctatttaat agtaagggaa gcttgcggga
     5521 gttagccaag tccgttgaca tcttcctgaa tggcgtaaat ttaaaagatc ttgaacagaa
     5581 gctgtgtttt attcacccag ttttgagaaa ttgaataaac tcaggatcca ggacgtcaaa
     5641 gccccaagga gtaaaaaacc tttgagcttg gtctttattt tttttcatat agccggtaat
     5701 aaaattataa tctcttcccc aggcaatcaa acgggtaaag ggtctggcca gccagaaaca
     5761 tttcttttga ttttcgatat ttaaattgtt cttttccttg gcggggccac gttcggcttt
     5821 taagacttgc cgggcgatta agccggtgat ggcgcgaatg aacttcgagt agagcttgcg
     5881 gtcatcgagt ttgatcacca gatgaaggtg atttcccaca ttcactaaat gatagagacg
     5941 tatgcggcac tgtttagcat ggtgtcggat gattttatca atttttttag cgtgagtaga
     6001 atggagcatc gagcgggagc cgatagcttg agtggatttt aataccaagt gcaggggttc
     6061 tttgatggat agggggcgcg atctttttgc atttcccttt agcaaaagcc cgccaaagtt
     6121 tcggggtatt ttcttattaa aaaagcaaag ctgttgcgct ttatttttta gcttcgtttt
     6181 cttgtttttt ggaggactgc ttttttttga cgccattcac tctgtttccg agaaatcttt
     6241 tatcgtgaaa agtggagcga tgtcaatttt aaaagttcca aatattagtt atttggaaga
     6301 ttaggggttt ggttgattgt ttaagaagtc tgatgatagt tgtaatttac cgctaccggt
     6361 ctttaaccgg cggttattta atgaatcaaa gaggttagtc cctat
//
LOCUS       DATBTE010000488        16968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1297102, whole genome shotgun sequence.
ACCESSION   DATBTE010000488 DATBTE010000000
VERSION     DATBTE010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 16968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16968
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297102"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="VIG33_18335"
     CDS             complement(<1..1257)
                     /locus_tag="VIG33_18335"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY8272858.1"
                     /translation="MERYIDLELLAVALGGIQNLPSNQELSKIISETEVNLLISQGAV
                     SNSLIEVAWYLFTIASIKSANEIYGIKRKRAALQVAGHIFDLALVSNKHESHAKIRLV
                     FASQIAYLKSQHQPNAIAIRKKHMREQEKFSLLPENYKELSLNCGVALLAFDIGYVYD
                     LTAGLEEQATSLAREWGVTSLYNTPFGAAFGLALGCRSLINFLVYGRDSSFQKAKSCF
                     LQAANCTSAYEDTDCIWMATLLHGLADELYATSIWKVLPADVPYHVKRAFTLGSPRVL
                     SLWPPQIDYLGQHNPFADEARFQFLSTPTSGGKTLLAQVLIAHHLSTRLTSACYIAPT
                     RSLCREVAASMKDRLRFLNLRSQIREAIEDFDLEENDIIEPSIDILTPESLAGLLRRD
                     PAAVYNKYGLFVFDEVHAVGDIGRGWT"
     gene            complement(1242..2060)
                     /locus_tag="VIG33_18340"
     CDS             complement(1242..2060)
                     /locus_tag="VIG33_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272859.1"
                     /translation="MPLNPKFTTTKPTAAGYVWVRQSHGNLVHQQALADDLAVHIVRS
                     KFSSATHIFQAWSQKHTTGQANAAPMKQILEATIKVCIGTQAQPKVVDHRQGWVAEHL
                     WYFLVKGSYPDGNVAMVFDVGMSPTDPGGDGLVIHRTGQDTFIFRLWEIKKVAGETDP
                     NATISRASGQLEEQGLAYLSRYVVNSQEKDLPQAEKEFINLLMDKWVAKSDDSAAGVS
                     VVCSAAKLTNPSFASLSTKFPKFSSNGTLEGRTVDLEDFVSFCEMVCESLWKGI"
     gene            complement(2184..2381)
                     /locus_tag="VIG33_18345"
     CDS             complement(2184..2381)
                     /locus_tag="VIG33_18345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272860.1"
                     /translation="MTFPSKESAGACEIISMGDSLEPALPENAEKAKKGLEMMDAIIS
                     SPLSKDQNPGPRTLKPLTVCY"
     gene            complement(2684..3061)
                     /locus_tag="VIG33_18350"
     CDS             complement(2684..3061)
                     /locus_tag="VIG33_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272861.1"
                     /translation="MSESLIKKIEDELKKRLPVRVAEDGYAPGEPQAFSEVERRVNSN
                     IKDLPFEKASEWEEAKNRCEEISIEIRKSAETIFSDPRRSGTPVEKIKPHYYDARVDE
                     LVENLKDKLEITTPNQDAAVYQD"
     gene            complement(3094..4134)
                     /locus_tag="VIG33_18355"
     CDS             complement(3094..4134)
                     /locus_tag="VIG33_18355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272862.1"
                     /translation="MNHLHFVSFLETLAQGNDPKNIALNASVKKQLSEMDIGVGIFNP
                     NSEQIEGFMGSSIETIQPFVFAAFDQKGPEVTTPEEELSLPFKNTWIETFDHSLFREV
                     VHGSLETDGRGGIFITPLHSEEMKVSEIVKGRRVRITMMRDMVGALIIEPDEPKNPYQ
                     AVMYQETRLLAVHPDDQGEDLFQAAIKRYGNKAVIVENYAGSLYHSMAEGSYSSLGPE
                     GRLYRAAQYLLTTMRKSSTQAGTVRVDARAKVGTGQNRRLMKIKNLVIVVPKKQREGL
                     ENLIGSSKEIDWSHRWDVMGHWRKVGGIGKDRNGDYSVKGYTWVISHVKGPDEQPYIR
                     KTRFVQDDDETE"
     gene            4404..5471
                     /locus_tag="VIG33_18360"
     CDS             4404..5471
                     /locus_tag="VIG33_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272863.1"
                     /translation="MKNTDEKFLLVYDETNNARKIYVDNGKFNVGDVGDFVLGGLALP
                     PGYMEPPISELRTRMRLDAGIKDIKLRHIGKGRFLDLIDAKKMAPFLDWFLENELLIH
                     FSHIDPFYWALADIVDSLLTFSFFEECIGYQYILKSDLFESLNQNQAQCLDIIERYNF
                     PDIRDEDIPSFFAEIIMLVEMSNAILPDRLDALLVFLNEAMRVESLPFLSFEDPGVRG
                     MLMDKFGAFYRHRLVLFKNSVHVLDEELEVKAFLENDIILSVFPEIDFKFVKSDDDSR
                     VQISDLIIGLLGKLFTDLGRFSLEEIVKQAAAISAQGKTNLVTLDKIISRSESLTQAF
                     HHHVCSGYKRNKFEKLLLEFK"
     gene            complement(5698..5946)
                     /locus_tag="VIG33_18365"
     CDS             complement(5698..5946)
                     /locus_tag="VIG33_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272864.1"
                     /translation="MATRKTKKKQDVLAKLFSKRDENILKATNWNPWEIEEELKRAKS
                     ARASFLEINPHHDLSIIDEHIDQLTTSLKIKTEKTSQI"
     gene            complement(5933..8887)
                     /locus_tag="VIG33_18370"
     CDS             complement(5933..8887)
                     /locus_tag="VIG33_18370"
                     /inference="COORDINATES: protein motif:HMM:NF042814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEY8272865.1"
                     /translation="MLTKDRAKLEILKLVARFEEHKISYQSSDYNETQTRRDFIDPFF
                     RALGWDIDNQGSIAEAYREVIHEDKIKISGHLKSPDYSFRINGKRYFFVEAKKPSVDL
                     KHAPEPAYQVKRYAWSAKLKISIVTDFEEFAVYDCTVKPDPDDKSTKARIDYFTCKEY
                     ENKFDFIWNTFSKEAVTLGRYDSYIQIESKKGTSSVDDDFLESLDQWRTVLAKDIFKN
                     NPKISEIDLNFAVQHTIDRIIFLRICEDRNIEPYGTVQKAIEAKNAYGALIKIFKTAD
                     EKYNSGLFDFSKDTLSPSIRISEECLKSIIGNLYFPNCPYEFSVIPVEILGSAYEQFL
                     SKTIQISTTGKIKIELKPEVKKAGGVFYTPQYIVDYIVANTLGKLIDGKTPQQIEKFR
                     VVDPASGSGSFLLGAYDFLLKYHQSWYSKHSSKSKGKKNDPLNPDGSLSTEVKKKILL
                     NNIFGIDLDANAVEVTKLSLLLKCLEGETQASIKAQLSFFHERVLPTLDENIKCGNSL
                     IDTDYYEIETDSDLLKQSKPFNWKREFSKVFSQGGFDVVIGNPPYIRIQRVPHSNLDY
                     FFNTYSTPTSKMDLSLLFLEKSFQLIRTRGLIGLICTSQWLTADYGTKIREFLATGKL
                     HEIMDFGSLPVFKDASTYPAVLILSDTPSKDLNVKKILTRDQLHFDGITKIRGFSVAL
                     EALSEKPWSLGALDITRCPLPISPLSKFGKAFIGTKSGLNEAFVLTKAEAKKLKIEKK
                     IILPYAYRGGEVESFKILEPESVIIYPYIKNSESTPKLMTEKYLKENFPNAYAHLLSK
                     KTALQKRQDSRKYYAKGTDWYRHLRAGTFQIVENKKLVVKGISLECNVGLFNGPSTFD
                     GARCPCIIIEQNKPYSEIYFVGILNSKLASYYLKSICPPKLNGYIEFTARALTNFPIR
                     VVDFSRAEEKKIYDDIVHCAISLLELNASENSDTRRIEHMKVQLDSLVYTLYGLTTEE
                     IRLIEGNQHGDS"
     gene            9068..9817
                     /locus_tag="VIG33_18375"
     CDS             9068..9817
                     /locus_tag="VIG33_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272866.1"
                     /translation="MSSREREQGFLAPQQKMVAGLDMNCVYDFFASIGSGWINMATIV
                     GALVGIAISIYAILFSKKLSDESTIVAASIRANTDAIQKQTAAIHNHTRILRLDNLRN
                     LCELSRGDERRHSYWHFRWRFETYDVLGGQIQTKDGEDVIFVGHCDLRVEGFSNRPEQ
                     IGFEGRESEFYTAKIANIEAAEGQTLPFQNGDVVACFDQNGELMISKGVDPWTMEPKF
                     IFMVVRLGPFQHGHGERTIGTLPVFPRIKDS"
     gene            9837..10271
                     /locus_tag="VIG33_18380"
     CDS             9837..10271
                     /locus_tag="VIG33_18380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272867.1"
                     /translation="MNREDLYSAQNSSDHFWLPAFTLILCRNSNARQKVTDLPVREPI
                     TRHLSDRLNNPLFTKMGDQQTLRSSVAVRNLRGSEPRFSLLLQWDSKLDQSVSDGGPR
                     GKSFLRNLGYGTAIFDVFFFEESFGSPRFGVNKINHLNEMWF"
     gene            10424..10753
                     /locus_tag="VIG33_18385"
     CDS             10424..10753
                     /locus_tag="VIG33_18385"
                     /inference="COORDINATES: protein motif:HMM:NF018050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY8272868.1"
                     /translation="MESRVFIEISIFKNKVDALGGVDLLRAIQTEILKDPSKGDIVQG
                     AGGIRKIRVARAKSGKSGGYRVFYLDLPEKGQTYLMTILDKRDSENISDEEKSLLKAF
                     AKKIKEK"
     gene            10756..11094
                     /locus_tag="VIG33_18390"
     CDS             10756..11094
                     /locus_tag="VIG33_18390"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY8272869.1"
                     /translation="MKKTKLGQELLEGMQEIIAHQEGKITLRTSELEMLAPPPDVSAK
                     DVAKIRSRNLHMSQGAFAKLLGVSVRVIQSWEQGDKKPSGAARRLLQVASRDPQALLS
                     AATPRKTKAG"
     gene            11136..12299
                     /locus_tag="VIG33_18395"
     CDS             11136..12299
                     /locus_tag="VIG33_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272870.1"
                     /translation="MGYHIKFLPLKKSNPQWKVQFISFKKCDTQNSKAKKPKREWDIS
                     KDRWLSLGFSESMNLVEAKGRARQLNAQNQLKRQEEQIVKINEQQRQFKLRHDSVLPS
                     EIVAEFEKVFVRKSDSQTELGLRRTSRADVVWRAAQRMIVAVGVEPSHWVYHSRDIYE
                     YMISQKMSVRYAHSVLSMANWWGYFVSHKMARPFLPVKSPRGYDRMRLIEANCQKITG
                     VTRASKQITPKDLEKTKLQMNQLNFNWLFVSVWFGLRPKEIDSLHNSNMWKIEKLANG
                     RKILWVFQTKIIALPPEDRWKPIPIIFSEQKFALRIVESGKLKRPLMKTVTKNFGKGT
                     TTYGGRKGFPDLMLSKGQSFENISVWMGHSTLQRTWKSYKSRRRFHLAGYPTW"
     gene            12385..12987
                     /locus_tag="VIG33_18400"
     CDS             12385..12987
                     /locus_tag="VIG33_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272871.1"
                     /translation="MMVSADPKKIRTTIRRYERNFEKDPTYRDGGGTRFLIGPYYLLI
                     GDVAGAMEHYHWFQENFADSSDEPIHTLSWALALFRTGQVNDAVFRLRCAHLSNPYMI
                     PALLKIPHGQPSVRRGSNWEEELYIMETSAPFFEVWAESEQAWLRQTWNSPEFTALVK
                     EHISIRKALEAVPVGPRRTELVERLFSLAETSAITGEGSH"
     gene            12987..13886
                     /locus_tag="VIG33_18405"
     CDS             12987..13886
                     /locus_tag="VIG33_18405"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY8272872.1"
                     /translation="MPIIQLQNDPSKSQYPAFLVIPAGEFADQRFDMQMNVCDNPTCT
                     CTDIHIALFNQKGGALPIQFKVDLDLKEKVQTPQGKATREDLLAKTVAKSFDKSDWKY
                     LWSVFSGKKAAHTSNFDPHTTYYPFPMSRDIEEHSELVAFSQVFPHAEKFIIELSGET
                     ILLDEQYCLTRGCACDQVLAVFILLENENGVLTQKNGEAPTMFLSHRTGTIGAVDVGD
                     FPEADLRSVLNAFLAKTPDLMAVFKKRHEQLSIMYEEYWRRNYKTVQAKTPKIGRNEA
                     CPCGSGKKFKKCCMLSALGDDIA"
     gene            complement(14041..14718)
                     /locus_tag="VIG33_18410"
     CDS             complement(14041..14718)
                     /locus_tag="VIG33_18410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272873.1"
                     /translation="MIQPEDAHYKEIRALGRRLNQRLVRLLSRRNIEESGKRLGVFRH
                     GTLALDTEDEITMVMDFGIHDLRHDGKNAVDRLLSSPESELSSEELAYLTSLSKAFYS
                     LFLAQENKPGIGVRVLDLVTEKERFIFDIGFSETARKGAILATRIFTHEGITMTTGAA
                     LPAGMDRPSISHAVARAKEAGRLDESGIFRIPFKVTAEIIRELLNNGAAEVIRYQDIP
                     PEPPLHE"
     gene            15298..15771
                     /locus_tag="VIG33_18415"
     CDS             15298..15771
                     /locus_tag="VIG33_18415"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HEY8272874.1"
                     /translation="MSVFNVKALILSAVLFSVMVTHAGPVAKVSDLDWLAGKWVGDFQ
                     GLPMEAYYTTASGGIILGQTKISNGNTAEFFEFEKIEQANDSLVLQPMPFGKPGVTFD
                     LKEMGANRVVFENPQHDFPTRIIYELKSKGQLLGRIEGIQNGQSVVQDFLFSKAQ"
     gene            15785..16129
                     /locus_tag="VIG33_18420"
     CDS             15785..16129
                     /locus_tag="VIG33_18420"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY8272875.1"
                     /translation="MAKYMINICYGDLEKRKKASLSNDDQFVMSKYREWSEKIAAKTI
                     VAHKLKDGDGRKVEFADGRTKDGPYVETKETIGGFYIIEVSSYSEAVETAKECPTLLY
                     QGGYVEVREVEF"
     gene            16154..16498
                     /locus_tag="VIG33_18425"
     CDS             16154..16498
                     /locus_tag="VIG33_18425"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY8272876.1"
                     /translation="MNEYMLVMKGDYRSWSSVSADEKQRIMEKYYAYVNRLKREDRFK
                     GGSALKHGGVQLRSEKGIVVVDGPFAETKETFNGYFIFEAQNYDEAVSIARECPALTH
                     GEVVEVFELTSH"
     gene            16508..>16968
                     /locus_tag="VIG33_18430"
     CDS             16508..>16968
                     /locus_tag="VIG33_18430"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEY8272877.1"
                     /translation="MRQSQVQATLDHLFRRESGRIVAYLTRFLGPDHIDLAENVVQDA
                     LVKAIQTWPLQGIPDNPAAWILQVSKNSAIDSIRSGKFVSHDSELVDSIQSSMTESST
                     DSGFENEIRDDQLKLMFICCHPIGLLLVTEDLFGKGLGRRIYNLVEHYVKM"
BASE COUNT         4654 a         3516 c         3790 g         5008 t
ORIGIN      
        1 cgtccaccct cgaccgatgt caccaactgc gtgaacttca tcaaatacaa ataggccata
       61 cttgttgtag acagcggcag gatcacgcct caacaggcca gccagactct ctggagtgag
      121 gatatcaata cttggttcaa tgatgtcatt ttcttcgaga tcaaaatctt cgattgcttc
      181 acgtatttga gatctgagat ttagaaaccg cagtcgatcc ttcatggatg ctgccacctc
      241 tctgcataaa cttctagtag gggcaatgta gcacgcggag gtcagcctag tggaaagatg
      301 atgtgctatc aatacttgag ccaacagtgt tttgccaccg cttgttggag tcgataaaaa
      361 ctgaaatctg gcttcgtcgg caaatggatt gtgctgtcct aggtagtcta tttgcggagg
      421 ccaaagtgaa aggacacgcg gactgcccag agtgaatgct cgctttacat gatagggcac
      481 gtctgcgggg agaactttcc agatacttgt ggcataaagt tcatccgcta ggccgtgcaa
      541 gagtgtcgcc atccaaatgc agtcagtatc ttcatatgct gacgtgcaat tcgcagcttg
      601 tagaaagcag ctcttagctt tttgaaatga agagtctctg ccgtaaacga gaaagttgat
      661 taagctacgg cagccgagcg caagaccaaa agctgcaccg aatggtgtgt tatagagcga
      721 agttacaccc cattctctag ccaaagacgt ggcctgttct tcaaggccag cagttagatc
      781 atagacatat ccaatgtcga acgccaggag tgcgacacca cagttgaggg atagctcctt
      841 gtaattttct ggaagcaaag agaacttttc ctgttctcgc atgtgttttt ttctgatcgc
      901 tatcgcattg ggctgatgtt gggattttag atatgcaatt tgtgaagcga aaaccaaacg
      961 aatcttcgca tgactttcgt gtttgttcga aacgagagct aagtcaaaaa tgtgtcccgc
     1021 aacttggagt gcagctctct tgcgttttat accgtagatt tcattggcac tcttaatgga
     1081 cgctatggtg aacagatacc atgcgacttc aataagagag tttgataccg ctccctggct
     1141 tataagaaga ttaacttcag tttccgaaat gatcttcgag agttcttgat ttgaaggtaa
     1201 gttttgtatt ccgcccagcg cgacagctag gagttctaag tctatatacc tttccatagg
     1261 ctttcacaga ccatttcgca gaaactaaca aagtcttcta gatcaactgt gcgtccttcg
     1321 agtgttccat tggatgagaa ctttggaaat ttagtggaga gtgacgcaaa tgaaggattt
     1381 gtaagcttcg cggcagaaca aaccacagaa acaccagcag cagagtcatc gctttttgcg
     1441 acccacttat ccatgaggag gttaataaac tccttttctg cctgtggtag gtccttctcc
     1501 tggctattca caacatatcg agaaagataa gcgagtcctt gttcttcaag ttggcctgat
     1561 gctcttgaaa ttgtcgcatt tggatcagtt tcgccagcga ccttctttat ttcccaaagt
     1621 cgaaaaataa aagtgtcctg accagttcta tggatgacga gtccatcgcc gcctggatca
     1681 gtcggagaca tgccgacgtc aaaaaccatc gctacgtttc catctgggta ggagcccttc
     1741 actagaaagt accagagatg ctcagctacc catccttgcc gatgatcgac gaccttgggt
     1801 tgtgcttgag tgccaataca tactttgatt gtggcttcaa gtatctgctt catgggtgcg
     1861 gcatttgctt gacctgttgt gtgcttttgt gaccaagctt gaaagatgtg agtagcggat
     1921 gaaaatttcg atcttacaat atggaccgcg agatcatctg cgagtgcctg ttggtgaact
     1981 aaattgccat gagattgcct aacccaaacg tatcctgccg ctgttggttt ggtggtggta
     2041 aactttggat ttagtggcat gcatctccca gtccaaccgc cttgccccaa gtgagataga
     2101 gcttttacgt tgttggaatt actcgcaaat agatcggcat ttagtcagat taagtttagg
     2161 ttcggcaccc gtaggacgta ggtttagtaa cacactgtta aaggcttcag ggttcttggt
     2221 ccgggattct gatctttgct tagtggcgaa ctgataatcg catccatcat ttccaagcct
     2281 ttcttagcct tctcggcgtt ttctggaagc gctggttcta aactatcgcc catcgaaata
     2341 atttcacagg ctcccgcaga ttccttagag ggaaaagtca acgatggaaa ttcttcctac
     2401 atttttgtct cttcaaaaag gctcacagat tttggaaaaa tatcagctcg tcctatggga
     2461 tatgtattca aagagcaaaa ttggagcaat ttctttgacg gcctcttttg gatcaaaact
     2521 gagcatccac ccacatatta acattccccc gcaacaggct cggcacagcc cgtaaggctt
     2581 ttcgaggctt tacttgcaac atcccgccga atccagattt cgagtttgtt gtctttatgg
     2641 acgatggcct tctgaatgat gagctggatg atatcccgtt tttttaatct tgataaacag
     2701 cggcatcctg gtttggcgtt gtaatttcaa gcttgtcttt taagttttca accagctcat
     2761 cgaccctggc atcgtagtaa tgtggtttga ttttttccac aggcgtgcca cttcgacgag
     2821 gatcactaaa tattgtttct gcgctctttc ttatctcaat tgagatctct tcgcacctat
     2881 tcttggcttc ctcccattct gaggcttttt caaaaggaag atcttttatg ttcgagttaa
     2941 ctcgccgctc gacctcagaa aatgcttggg gctctcccgg cgcatagcca tcctcggcca
     3001 ccctgacggg gagacgcttt ttgagttcgt cttcgatttt ctttattaag ctttcactca
     3061 tagctcctcc tgtggaaaga aatatttttt tagttattcg gtttcgtcat catcttgtac
     3121 aaaacgcgtt tttcggatgt aaggttgctc gtcaggtccc ttaacgtgtg aaatgaccca
     3181 agtatagcct ttcacggaat aatcgccatt tcgatctttt ccaattccgc cgacttttct
     3241 ccaatgaccc attacgtccc aacgatgaga ccaatcaatt tcttttgaag atccaatcag
     3301 attctcaagt ccttcgcgct gtttcttagg caccacaatt actaagttct tgattttcat
     3361 tagtcgacgg ttttgcccag ttccaacctt agcccttgcg tccacgcgca cagttcctgc
     3421 ctgtgttgag ctttttctca ttgtggtgag taggtattgg gccgcccgat aaagccgtcc
     3481 ctcaggtcct aacgaagaat aagatccttc ggccattgag tgatataacg acccggcgta
     3541 attctctaca ataacggctt tgttgccata gcgtttgatt gctgcttgaa ataggtcttc
     3601 gccttgatcg tctgggtgaa ctgcaagtag cctagtttct tgatacatca ctgcctgata
     3661 aggatttttt ggttcgtctg gctcgattat tagcgcaccc accatatcgc gcatcatggt
     3721 gattctcacg cgcctgcctt taacgatttc ggagactttc atctcttctg aatgaagagg
     3781 agtaatgaag attccccctc tcccgtctgt ctctagtgag ccatgaacaa cttctctgaa
     3841 taacgaatga tcgaaagttt caatccatgt attcttaaat ggcaaactaa gttcttcttc
     3901 tggagtcgtg acttctggtc ccttttgatc gaacgcagca aagacaaaag gttgaatcgt
     3961 ttcgatcgaa cttcccatga acccttcgat ctgttctgaa ttgggattga aaatgccgac
     4021 tccgatgtcc atttctgaaa gttgtttttt cactgaggca tttagtgcaa tatttttggg
     4081 atcgttacct tgagcaaggg tttcgagaaa actaacgaaa tgtagatggt tcatgctggc
     4141 tcccatgtca acacctatcg gtttaatttt ttaaacttca agagctgggt gtcatataac
     4201 tgtataaacg tctagcttgg gcgtgggata ttacagtagc tgcaatccgg accaaaaaga
     4261 acgcaacgat ttcatctttc taggtacgcc gatgttatga caaaatagcg catacaaggg
     4321 agtttaaatt gaacattccg agcattgatg acatcagagg ttacaatttg tggttaagag
     4381 attcggaaat ttccacacat aaattgaaaa ataccgacga aaaattttta ctcgtttacg
     4441 acgaaactaa caacgcccga aagatatatg tcgacaacgg aaagtttaac gtgggcgatg
     4501 tcggcgattt cgtcttaggt ggtttggcac ttcctcccgg ttatatggag ccacccatct
     4561 cagagttgcg tactcgcatg cgattggatg ctggtatcaa ggatattaag ttgcgccaca
     4621 taggcaaagg tagatttcta gatttgatag acgccaaaaa aatggctcca tttcttgatt
     4681 ggtttttgga aaatgaattg ctcattcatt tttctcacat cgacccattc tattgggcgc
     4741 ttgcagacat cgtagattct cttttaactt tttcattctt tgaagagtgt attggctatc
     4801 aatacatttt aaaatcagat ctgtttgaga gtctaaatca gaaccaagct cagtgtcttg
     4861 atatcatcga aagatataac tttcctgata ttcgtgacga agatattccc tcattttttg
     4921 ctgagattat tatgctagtc gagatgagca acgcaatttt accagatcgt ttagatgcac
     4981 ttttggtttt cctcaacgaa gcaatgagag tagaaagctt accgtttttg agctttgaag
     5041 atcccggcgt gagaggaatg ttaatggata agtttggtgc gttctacagg catcgtcttg
     5101 tcttatttaa aaactctgtg cacgtgctcg atgaagaact cgaagtgaaa gcctttttag
     5161 aaaatgatat aattctttcg gtctttcctg aaattgattt taagtttgtg aagtctgatg
     5221 acgatagtcg cgtgcagatt tcagatttaa tcattggcct gttgggcaaa ctctttaccg
     5281 atcttgggcg attctcgctt gaggaaattg taaaacaggc ggccgctata agcgcacaag
     5341 gtaaaaccaa cctcgttact ttggacaaga ttatttctag gtcggaaagt ttgacgcagg
     5401 cattccatca tcatgtatgc tccggctata aacgaaacaa gtttgaaaaa ttgcttcttg
     5461 aattcaaata gttatcgaca gcaaatccaa ggctatagcg attgttgtgg ttatgcgtcc
     5521 ttccctgata ttgacttgtg cgcaaaatcg aggcagtgtt tcggaaggct atgagagtta
     5581 gcctttcctt ccgcgtaaag actaatggtc aaatcttatt cattttcagc gtgggctgga
     5641 ttggaccttt aaaggaatct gaaacggatt ttacagatac gcgcttagcg ctttgaatta
     5701 aatttggctg gtcttttcag ttttaatttt cagagaagtt gtcaactgat caatatgttc
     5761 atcaatgata cttaaatcat ggtggggatt tatttccaga aaacttgcac gagctgattt
     5821 ggctcttttt aactcttcct caatctccca tgggttccaa ttcgtggcct taagaatatt
     5881 ctcgtcacgc tttgagaaaa gcttggctaa tacatcttgt ttctttttag ttttacgagt
     5941 cgccatgctg gtttccttca atcaatctga tttcttctgt agttaggcca tacaatgtat
     6001 acaccaggct atcaagctgc actttcatgt gctctattct cctagtatct gagttttcag
     6061 atgcattcag ctccaaaagt gatatagcgc aatgcacaat gtcgtcgtag attttctttt
     6121 cctcagctcg tgaaaagtct acaactcgaa tcgggaagtt agttaatgct ctggctgtga
     6181 actcgatata accgtttagc tttggaggac atattgattt caaataatag ctggccagct
     6241 ttgaatttaa tattccgaca aaatatattt cggagtaggg cttattctgc tcaataatta
     6301 tgcatggaca tcgtgccccg tcaaacgttg aaggtccatt aaataacccc acgttgcatt
     6361 ccagtgagat ccctttcact accaattttt tgttttcaac aatctgaaag gttcctgcgc
     6421 gcaaatgccg ataccaatct gtacccttag cataatattt tcgactatct tgcctttttt
     6481 gaagagcagt tttcttagat agtaaatgtg cataagcatt cggaaaattt tctttcaaat
     6541 atttttctgt cattaacttt ggggtggatt cactattttt gatgtaagga taaattatca
     6601 cagactctgg ctctagaatc ttaaatgatt ctacttcgcc acctcgatat gcatatggga
     6661 gtatgatctt tttttcaatt tttaattttt ttgcctcggc tttcgtaaga acaaacgctt
     6721 cgtttaaacc agatttagtt cctataaatg cttttccgaa tttgctgagt ggtgagattg
     6781 gcaacggaca cctggtaatg tccaatgctc ccaatgacca gggcttttct gaaagcgctt
     6841 caagggcaac tgaaaagcct cggattttcg taataccgtc gaaatgcagt tggtctctag
     6901 ttagaatttt ttttacatta agatccttac ttggtgtatc gcttaaaata agaacagctg
     6961 ggtacgtgct agcgtctttg aataccggga ggcttccaaa atccataatc tcgtgaagtt
     7021 ttcccgttgc cagaaactct ctaattttcg ttccatagtc ggcagtcaac cactgggacg
     7081 tgcaaattag accgatgagg ccacgggtcc ttattagctg gaaggacttt tcaaggaaaa
     7141 gaagtgagag gtccatcttg cttgtaggcg tggaataggt attaaaaaaa taatctaaat
     7201 ttgaatgagg aactctttga atccgaatat acggaggatt gccgatcacc acatcaaaac
     7261 cgccttgact aaaaaccttt gaaaattccc ttttccaatt aaatggtttt gactgtttca
     7321 gaaggtcgct gtccgtttca atttcataat agtcagtatc gatgaggcta ttgccacatt
     7381 ttatgttctc atcgagggtt ggcaaaactc gttcatggaa aaaactcaac tgcgctttaa
     7441 tgcttgcttg ggtttcacct tcaagacatt ttagaaggag gctcagttta gttacttcga
     7501 cagcatttgc atcgaggtcg atgccaaaaa tattatttaa taaaattttc ttttttactt
     7561 ctgtagatag cgagccatct ggatttagag ggtcgttttt cttgcccttg gatttcgatg
     7621 agtgcttgga gtaccaactt tggtggtatt tcaaaaggaa gtcgtaagcg cctaataaga
     7681 agcttccgct tccgctagca ggatcgacaa ctctaaattt ttcaatttgc tgtggcgttt
     7741 ttccatcgat caacttgccg agggtattcg cgacgatata gtcaacaatg tactgaggcg
     7801 tatagaatac gccaccagcc tttttgacct ccggttttag ctcgatttta attttgccgg
     7861 ttgtgctaat ttgaatggtt ttactgagaa actgttcgta ggcgctgcct aaaatttcga
     7921 ctggaattac tgaaaattca taaggacaat tgggaaagta aagatttcca atgatcgatt
     7981 ttagacactc ttctgatatc ctaatcgaag gtgaaagcgt atctttgcta aagtcaaaaa
     8041 gacccgagtt atatttttcg tcagcagttt taaaaatctt tatcaaagct ccataggcat
     8101 ttttagcttc tatcgccttt tggacagttc catatggttc gatatttcta tcttcgcata
     8161 ttcgtaaaaa tatgatccga tcaattgtat gctgaaccgc aaaattcaaa tcgatttcgc
     8221 taatctttgg attgttttta aaaatgtctt tagctagaac cgtgcgccat tggtcaaggg
     8281 actctaagaa atcatcgtca actgagcttg tgcccttttt tgattcaatc tgaatgtaag
     8341 aatcatatct tcctaatgta acggcttctt tagaaaaagt gttccaaatg aagtcgaatt
     8401 tattttcata ctctttgcac gtaaaataat cgattcttgc tttagttgat ttgtcatcgg
     8461 ggtccggctt gactgtacaa tcatagaccg caaactcctc aaaatcggtg acaatgctaa
     8521 tttttaattt tgcagaccat gcataccttt tcacctgata ggctggttca ggggcatgtt
     8581 ttaagtcaac agaaggcttt ttagcctcga caaaaaagta tctttttcca ttaattcgaa
     8641 acgaatagtc tggcgattta aggtgcccgg aaattttgat tttatcttca tgaataactt
     8701 ctcggtatgc ctccgcgatg gacccttgat tgtcgatgtc ccagccaaga gcgcgaaaga
     8761 atggatcaat aaagtctctt cttgtttggg tttcattgta gtctgagctt tggtatgaaa
     8821 tcttatgctc ttcgaatcgc gcgaccaatt ttaagatttc taacttagcg cggtcttttg
     8881 tcaacatagc tttgtcgctt tccatccatt ggtcggcata atctgatcaa ctcattttga
     8941 aagatattag cgcatatagc aatgcacagg ctattgtttt ctttttgatt gtggtattca
     9001 tgctggcata cgattgtcat tactcgataa aaggccttgt gcgtgaaaat taaacactgg
     9061 tggtggggtg agcagtcgtg aacgcgaaca aggcttccta gcgcctcagc aaaaaatggt
     9121 ggcaggtttg gatatgaatt gcgtatatga tttcttcgca agtataggtt ccggctggat
     9181 caatatggcg acaattgttg gtgcgctggt aggaattgct atttcaattt atgctatctt
     9241 gttctcaaaa aaattgagtg atgaaagcac aatagtagca gcctctatca gagcgaatac
     9301 ggatgcgatt caaaagcaaa cagcggccat tcacaatcac acaaggattc ttcgtctgga
     9361 taatttgcga aacctctgtg aattatcgcg cggcgatgag cggcgacata gctactggca
     9421 ttttcgctgg agatttgaaa cttatgatgt gcttggtggt cagattcaga caaaagatgg
     9481 tgaagatgtg atatttgttg gccattgcga tttacgagtt gaaggtttca gtaatcggcc
     9541 cgaacaaata ggctttgaag gaagagagtc ggaattttat actgccaaaa ttgccaatat
     9601 tgaagctgcc gaaggccaga cactaccatt tcagaatggc gatgtagtgg catgtttcga
     9661 tcaaaatggc gagttgatga tatccaaagg tgtcgatcct tggacgatgg agccaaagtt
     9721 tatcttcatg gtggtccgtt taggaccttt ccagcatggc cacggcgaac ggacaatagg
     9781 cactttgccg gtatttcccc gtatcaagga ttcatagtcc tcacggaagt tggtatttga
     9841 accgagaaga cttatactcg gctcaaaatt cgtcggatca tttctggctg ccagccttta
     9901 ccctgattct ttgtaggaac tccaacgctc gtcaaaaagt cacagatctt ccggtacgag
     9961 agcccatcac tcgacatctt tcggaccgac tgaataaccc gctgttcacc aagatggggg
    10021 accaacaaac cctccgatct tctgtagccg taaggaactt gcgcgggtct gagcccaggt
    10081 tttccttgtt gcttcagtgg gattccaaac tcgatcaaag cgtctctgat ggcggcccgc
    10141 gaggaaagag tttcctccgc aatttgggct atggaacggc catttttgac gtatttttct
    10201 tcgaggaaag ttttggatct ccaaggttcg gtgtgaataa aatcaatcat ctcaatgaga
    10261 tgtggttttg atttttctgg gaaggcccgc cattactact aataaaatca catagttaga
    10321 aagaaaaacc tcggctgatt cagccgaggc agccgatgcc aattcgtttc agtctaaaac
    10381 tttctctttt tctataccac tgagtatgct atactactaa gtagtggaat caagagtctt
    10441 catcgaaatc tcaattttta aaaacaaagt ggatgctctt ggtggagttg atcttctgag
    10501 agctattcaa acagagattt taaaagatcc cagtaaaggt gacatcgttc agggtgcggg
    10561 tggtatccga aaaatcagag tggcgcgagc gaaaagcggg aagagtggcg gataccgagt
    10621 gttttacttg gatcttccag aaaaaggtca gacctacttg atgacgattt tagataaaag
    10681 ggattcggaa aatatctcgg acgaagaaaa atctctactc aaggctttcg ctaaaaaaat
    10741 aaaggaaaag tgattatgaa aaaaactaaa ttgggccaag aacttttgga aggtatgcaa
    10801 gagattatcg ctcatcaaga gggtaaaatc actcttcgaa cttctgaatt agaaatgtta
    10861 gcgcccccac ctgatgtgtc tgccaaggat gtggctaaaa ttagatctcg gaatctgcac
    10921 atgagccaag gggcctttgc aaagctttta ggggtcagtg tccgagttat tcaatcttgg
    10981 gagcaggggg ataaaaaacc atctggcgcc gcaaggaggc tcttacaagt agcatcccgt
    11041 gatcctcagg ctctgttatc agcggcaact cctcgtaaaa ctaaagcagg ctaatctaaa
    11101 agccgatgtg gatctcaaaa acaggaggtc cgcaaatggg ttatcatatc aaatttttgc
    11161 cacttaaaaa gtcgaatcca caatggaaag ttcagttcat ctctttcaaa aaatgcgata
    11221 cgcaaaattc gaaagcgaag aagccaaaac gcgaatggga tatttctaaa gatagatggt
    11281 tatcgctcgg attttctgaa tcgatgaatt tagtagaggc caaaggtcga gctcgtcagc
    11341 taaatgcgca aaatcaattg aagcgacaag aagaacagat agtaaaaatc aatgagcagc
    11401 aaaggcagtt taagttgagg cacgattcgg tgctgccatc agaaattgtc gcggagtttg
    11461 aaaaagtctt tgtcagaaaa agtgacagcc aaactgagct gggattgagg cggacctccc
    11521 gagccgatgt tgtctggcgg gccgcacagc ggatgattgt tgcagttggc gttgagccat
    11581 ctcactgggt ctatcacagc cgcgatatct acgaatatat gatctcccaa aaaatgagtg
    11641 tccgttatgc ccattcggtt ttgtcgatgg caaactggtg gggatacttc gtctctcata
    11701 aaatggcacg tccctttctg ccagtaaaat cgccgcgagg ttatgatcgg atgaggctga
    11761 tagaagccaa ttgccagaaa attactggcg ttacaagggc gtcaaagcag atcactccaa
    11821 aggaccttga gaaaacaaag ctgcagatga atcaactgaa cttcaattgg ctctttgttt
    11881 cagtctggtt cggacttcgc ccaaaggaaa tcgacagcct tcacaattca aatatgtgga
    11941 aaattgaaaa attagcgaat ggacgaaaaa tcctctgggt tttccaaaca aaaattatcg
    12001 ccctaccacc cgaagatcgt tggaaaccga tccccattat tttttcagaa cagaaattcg
    12061 ctcttcgaat tgttgaatct ggaaaactca aacggccgct catgaaaact gtaaccaaaa
    12121 attttggaaa gggcactacg acttatggtg gcagaaaggg ttttcctgat ctgatgcttt
    12181 caaaaggaca gtcgtttgaa aatatcagcg tatggatggg acactctacg cttcagagaa
    12241 cctggaaaag ctataagagt cgccgccgct ttcacttagc ggggtatccg acttggtgag
    12301 agccttcggc ccctgcgccc catgccaaaa aatatgcggt aagtcactga tgacgacatg
    12361 gattttttcc ggtaggctgt cgtgatgatg gtttccgcag atcctaaaaa aattcgtacg
    12421 actattcgtc gttatgagcg caactttgaa aaagatccta cctatcgtga tggaggtgga
    12481 acgcggttcc ttattggacc gtattatttg cttatcggtg atgtcgccgg agcgatggag
    12541 cactatcatt ggtttcagga aaatttcgcg gacagttccg atgagcccat ccatacactc
    12601 agttgggctc tagctttatt ccgcacgggt caggtcaacg atgctgtatt tcgtttgcga
    12661 tgcgctcacc tgtcgaatcc ttatatgata ccagcgctgc taaaaatccc gcatggacag
    12721 ccatccgtta ggagaggcag caattgggag gaagaactct atatcatgga gacgtctgca
    12781 ccgtttttcg aggtttgggc cgagagtgag caagcctggc tacggcaaac ttggaacagt
    12841 ccggaattta ctgcgctggt aaaagagcat atatccatta ggaaggcctt ggaggcggta
    12901 cctgtcggac ctcggcgaac agagctggtg gagcgccttt ttagtcttgc agaaacatcc
    12961 gccataaccg gggagggaag tcactgatgc caattattca actacaaaac gatccttcca
    13021 aatcgcaata tccggccttt cttgtaattc ctgcgggcga atttgcagat cagcgatttg
    13081 atatgcaaat gaatgtttgc gataatccga cgtgcacatg tacagacatt cacatcgctc
    13141 ttttcaatca aaaaggaggg gcgctgccaa ttcaatttaa ggttgatctg gatttaaagg
    13201 aaaaagttca aacgccacaa ggcaaggcta cgagagagga tttgctagcc aagaccgtgg
    13261 ccaagtcttt tgataaaagc gactggaaat atctgtggag tgtctttagt ggcaaaaagg
    13321 cagcccacac ttcaaatttt gatccacaca caacctatta cccttttcca atgtctagag
    13381 acatagaaga acatagtgaa cttgttgctt tcagccaagt ttttccccac gcagaaaagt
    13441 ttattattga gctctcaggg gaaacgattc ttctcgatga gcaatactgt cttacgcgtg
    13501 gctgtgcctg cgaccaagtt ttagccgtct ttatcttgct tgaaaacgaa aatggtgtac
    13561 tcacccaaaa aaacggtgag gctccgacaa tgttcttaag tcataggacc ggcactattg
    13621 gcgctgttga tgtcggtgat tttccggaag cggaccttcg cagtgtgctg aatgccttct
    13681 tggcaaagac accggatttg atggcggtct ttaagaagag gcatgagcaa ttgtcaatta
    13741 tgtatgagga atactggcga cggaattaca agacagttca agcaaaaaca ccgaaaatcg
    13801 gtcgtaacga agcctgtccc tgtggcagcg gcaagaaatt taaaaaatgt tgtatgctgt
    13861 cggcattggg cgacgatata gcgtaaatac gctcattact aagaagcgat gggctgtaag
    13921 gattcaaata tcacttttta tccaatgaat atactttgga ggaatgctgc ttcaagaaat
    13981 agtgccgatc agtctctttg aagactcctt ttttcttggt atatttttat tgatgcggat
    14041 ctattcatga aggggcggtt ccggcggaat gtcttgatat cttattactt cggcagcacc
    14101 attattcaag agttcgcgaa tgatctcagc tgtgacttta aatggaattc taaaaatgcc
    14161 agactcatct agtcttcctg cttccttggc gcgcgccacg gcatgagaaa tactcggtct
    14221 atccatgcct gcggggagtg cagcccccgt tgtcatcgtg atcccttcgt gtgtaaaaat
    14281 ccgagtagca agaatggccc ctttgcgggc ggtctcactg aatccgatgt caaaaataaa
    14341 acgctctttc tcagttacca gatcgagaac tctcactccg ataccaggtt tattctcctg
    14401 agccaggaat aaggaataaa atgctttgct caatgaagtt aaataagcga gttcttcaga
    14461 actcagttcg ctttccgggg atgataaaag ccgatctaca gcgttttttc catcatggcg
    14521 caaatcatga ataccaaagt ccattaccat ggtaatttca tcttccgtgt ccagagccaa
    14581 agtgccatgt ctaaataccc ccagacgctt tccgctctct tctatatttc tacggctgag
    14641 gagacggaca agccgttggt taagcctgcg tccaagagca cgtatttcct tgtagtgggc
    14701 atcttcaggt tgaatcataa ggaattcctc cgagggagtc aaaatatcga aaagggagtc
    14761 atttgaaaac cctttttaac cgacatagtt gtcatgtcaa aactgtgtcg gaggccacaa
    14821 gagcgttgag gttcacttaa atagtctcaa attttaattt tgaaagatca attctagatc
    14881 cataggaata gcttttagca gtgttctgat ctctattggc taagtccact ttagttggaa
    14941 caaaaaggac gggtcgacct atcgggttca aattaaagaa ggcaaatatg aattcaggaa
    15001 aaaaatgaca gccgcccgaa attttctttg tttctcacag cgttgacatg gcgcttcaga
    15061 ttaggtaagt cacctctagt cgagtttggg gtgagtgaga cctcttgaga tgttggagaa
    15121 ttttaattcg tcctgattgt ttcgtatcta tttataggaa atcactattt tcgccggagt
    15181 aaaggggatt atgggctcat tcaaaaaaaa tttaaaagaa tttttgaacc attgtcgatt
    15241 ttggccctcc tcgttcgtcg ttctatcaaa cggacaaaca aaccagagga ggtatctgtg
    15301 tcggttttca atgtaaaggc tcttatttta agcgcggttt tattctcggt aatggtgacc
    15361 catgcgggtc cagtggcaaa agtcagcgat cttgattggc tagccggcaa atgggtcggc
    15421 gattttcagg ggctgccgat ggaggcgtat tacacgacgg catcaggtgg aatcatttta
    15481 ggtcagacaa aaatctctaa tggcaatacc gctgagtttt ttgaatttga aaagatcgag
    15541 caagctaatg attcgttggt tttacagcca atgccattcg gaaagcctgg cgtcaccttt
    15601 gatctcaagg aaatgggggc aaatcgagtg gtttttgaaa accctcaaca tgattttcca
    15661 actcgaatta tctacgaatt gaaatcaaag ggtcagcttc ttggtcgaat tgaaggcata
    15721 caaaacggcc aaagcgttgt tcaagacttt ttattctcaa aagctcaata gggcggagga
    15781 aacaatggca aaatatatga ttaatatttg ttacggagat ctagaaaagc gaaagaaagc
    15841 atccctctca aatgacgatc aattcgtaat gagcaaatat cgtgagtggt cagaaaaaat
    15901 tgcggcaaaa acgattgtcg cacataagct caaagatggt gatggccgaa aagttgaatt
    15961 tgcagatggt cgaaccaaag atggtcctta cgttgaaacg aaggaaacca tcggcggctt
    16021 ttatatcata gaagttagca gctatagcga agcagtagag acagcaaagg aatgtcctac
    16081 gctactctat cagggcggct atgttgaagt tcgtgaagtg gaattttaaa tttaaagtag
    16141 taggagaata tttatgaacg aatatatgtt ggtgatgaag ggtgactata ggtcctggag
    16201 ttctgtgagc gccgatgaaa aacaacggat catggaaaaa tactatgctt atgtaaatcg
    16261 actcaaaaga gaagatcgct tcaaaggtgg ttccgctcta aaacatggtg gcgtgcaact
    16321 ccgatcagaa aaggggatcg ttgttgtaga tgggcctttt gctgaaacaa aagaaacttt
    16381 taatggctat tttattttcg aagctcaaaa ctacgatgaa gctgtttcca ttgcccgtga
    16441 atgtccagca ttgactcatg gtgaagtcgt ggaagtcttt gaattaactt cccactagag
    16501 gaattgaatg cgtcaaagtc aggtacaagc aacattggat catttatttc ggcgtgaatc
    16561 aggccgaatt gttgcctatc tgactcgttt tcttggtccg gaccacatag accttgcaga
    16621 aaacgttgtg caagatgccc tagttaaagc tattcagact tggccccttc aaggaattcc
    16681 tgacaatcct gcagcgtgga tattacaggt ttccaaaaat tctgcgattg attccatacg
    16741 aagtgggaaa ttcgtatcgc atgactcaga gcttgtagac tcaattcagt cttcgatgac
    16801 cgagtctagc actgactcgg gatttgaaaa tgaaatacgg gatgatcagc ttaagttaat
    16861 gttcatctgt tgccatccta ttggtctttt gttggtgacc gaggatcttt ttggtaaggg
    16921 actggggcgc aggatctata acctcgttga gcattacgtt aaaatgat
//
LOCUS       DATBTE010000489        10031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1092806, whole genome shotgun sequence.
ACCESSION   DATBTE010000489 DATBTE010000000
VERSION     DATBTE010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10031
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092806"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1536)
                     /locus_tag="VIG33_18435"
     CDS             complement(<1..1536)
                     /locus_tag="VIG33_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY8272878.1"
                     /translation="MQQLKRKPKRSLRTILIVWFLLFSVIPLAFVTVYSMIKYEKALD
                     RELSQRLSGNAREISVIFSEYRAGLRQKRDKYIRDPNLIYHLSIGDGATIRGIASQWL
                     KQDFTSSLTFFARDGRMIVSVFRDENGEIRNFSPSSQAVFLNAKYISTFKNQKEVGSV
                     EFTEQHKTSLLLISRVTNGAGKIVGYFEQIIDLDKGFLNRLKARLKLEVLFFKETGQL
                     MMASHPDFYLYKKDFFEPYFKPNKEPFFDLNIRSMPYGFLISPLDWDSGKLYVGLGAS
                     KSEAKEVLRKVNFAFISVVVAIVILLFITILVTSNWVLKPLYELVGALQRFESSENSV
                     EIPVKNETEIGLLTASFNEMSRRISQARTELKKKIFELEAANQELKDTQAKLVHSAKM
                     ISLGQLVAGVAHELNNPIGFISSNMTHLRDYSEKLIKLVEMAEKDPENIPALKADFEY
                     DYIVQDMPKLIASCEDGARRTKDIVLGLRNFSRLEEAKLKEIDLHESLDTTLNLLAGE
                     IKNR"
     gene            complement(1553..2563)
                     /locus_tag="VIG33_18440"
     CDS             complement(1553..2563)
                     /locus_tag="VIG33_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272879.1"
                     /translation="MSEDLNRVPLSKKGQRQVTPFTGNELLYDYMSSMLDEERKKAVE
                     IYLAASKDAQLEFQKIQTGLQYAERMRQITVSPALEQRLAAPSSYLSVLFKKSRFDQW
                     PVGLKWGLEALVVVFVIVTFLTVAPWEKIMKLRLSMDTNDVVLTEVNKSSEAKKDLQV
                     AAATGQAAEAPQFVDEGIKANNKESVAVPPAAPLAPVEKATPKPLPIPKAQGSVVAKV
                     TEKAEEEESDENSEESIKTTGGYLYRGQIAVTNLEAVGPKIKEKIIELGGRKAGEVEL
                     GWQKTPGFAYFHFTIPEAKYSELQAFLSEYGKAKISKDKHPRVMPDGIIRLIISVEEA
                     KK"
     gene            complement(2568..3035)
                     /locus_tag="VIG33_18445"
     CDS             complement(2568..3035)
                     /locus_tag="VIG33_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 region 4 domain-containing protein"
                     /protein_id="HEY8272880.1"
                     /translation="MGEPEVKSIALFFFFAFLDDRRALEASTQALSIYSDKKSKNSQF
                     NNEVLIVMSTFSVWTRHHLKLNRGNPNVSTDAGWMIPADLDIGPWREFQKLATQDELL
                     AVIWSKVLEISEKSIAQGLGISEGTVRYRLGRALRKLGSMAQGVSTKKLNAVK"
     gene            complement(3044..4087)
                     /locus_tag="VIG33_18450"
     CDS             complement(3044..4087)
                     /locus_tag="VIG33_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY8272881.1"
                     /translation="MSIIEKFTDYFSNDIAIDLGTANTLVYVKGRGIILDEPSVVAVQ
                     KNYRGMQNRVLAVGKEAKDMLGRTPGSIVAIRPIKDGVIADFEVTQSMLKYFIAKSMG
                     EKKSFIRPRIIICVPYGITQVEKRAVKEAAQSAGAREVYLIEEPMAAAIGAGLPITEP
                     SGNMVVDMGGGTTGVAVISLGGIVYCKSIKVAGDKFDEAIINYVRRQFNLLIGERTAE
                     LIKIQIGNAYPFEDEKTMEIKGRDLVAGAPKTIEITSSQVNDALMDPLSEVVDAVRTA
                     LEKTPPELASDIVDNGIVLTGGGAMLANLDVLLRERTGLPVSIAEDPLSCVVMGSGKV
                     LDQLDLLRQLTID"
     gene            complement(4207..5841)
                     /locus_tag="VIG33_18455"
     CDS             complement(4207..5841)
                     /locus_tag="VIG33_18455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEY8272882.1"
                     /translation="MKRFSKRERLENIRKMQEEIFDLCIIGGGINGAGVARDAASRGM
                     RVALIEAKDFASGSSSRSSKLIHGGIRYLENLEFHLVFEALNERTRLFEMAPHLVHPL
                     RFMVPLYENSRVGMFKMGLGMWLYDALALFQAPEMHERLNAKESLTRMPALEKNKLSG
                     SYVYSDAYMDDDRLVHETLRSAHEMGAICANFAKAVGASFAANGKIDAVECEDQLDSK
                     KFKIRAKHVVSCVGPWTDDLGPKLLKGWQNILRPTKGIHLTLQKSRLPLSSAVVMAAE
                     KSDRIVFGIPRHEMVVIGTTDTDYRELPDGVVATADDVKYLLEVTHQYFPGAAITEKD
                     IVATYAGVRPLVKDQSSSEGKTSREHVIIDDERGVTLVAGGKYTTYRLMSEQVVCHSL
                     KFFSFDEKVRFSKNDTLKALNPYTNVDSFQQAMGMSALWAQESGRPEVITQKLAERYG
                     MEAAEILHRYPMQYNYWQLEAAQAIDHTLCLNLVDFYARRVPLFLARPDHGMPQLESI
                     CEVFKEKLAWPEAELQRQKEALLQYMERELAWKSFL"
     gene            complement(5847..6323)
                     /locus_tag="VIG33_18460"
     CDS             complement(5847..6323)
                     /locus_tag="VIG33_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272883.1"
                     /translation="MNLFCRVIQLEDLNEIIDFENKKLKELIPDEMERTLHSWNARWR
                     KEALEHYFPLGWSFLVRDPDKTSEFSDEGLLVGYYIAQPLLFLAGQTQSLWVEHLQYA
                     SLQARDELCELAYRISREKHFQKVFFPNVSGINNSISSFKAEIWNPDVMSIKTTKA"
     gene            6388..7131
                     /locus_tag="VIG33_18465"
     CDS             6388..7131
                     /locus_tag="VIG33_18465"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015089387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY8272884.1"
                     /translation="MKYKEYSKEIWNRINSTLDYALAKDSEPIAAFDADGTLWDTDLG
                     ESFFQYQIDHKLISLPDSPWEFYQELKKKDPSEAYLWLAQINHGQKLDVVHQWAERAL
                     EASAPVPLFEEQKKIVELFLSKGVKVFIITASVKWAVEPGAKILGLGRDSVIGIETQI
                     ENDLITQKQKGIITYQMGKVEALLERTNGKKPFFASGNTMGDFQLLNSASGLALAVSA
                     ANQDDRLFRAENDLQAIAEQKAWVRHRFI"
     gene            complement(7177..7506)
                     /locus_tag="VIG33_18470"
     CDS             complement(7177..7506)
                     /locus_tag="VIG33_18470"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY8272885.1"
                     /translation="MRLSFKEQEGIKNAIGACLQEIPFKLYLFGSRADDKKKGGDIDL
                     LLLVPASEKETVVELKNKIRIKIFETLSEQRIDITVAIEQECASDEFLRSILRGAIPL
                     SGRGSFK"
     gene            complement(7496..7918)
                     /locus_tag="VIG33_18475"
     CDS             complement(7496..7918)
                     /locus_tag="VIG33_18475"
                     /inference="COORDINATES: protein motif:HMM:NF020360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HEY8272886.1"
                     /translation="MDLTGLYYSKIVKSLKYLEYSYQRTLTMPLNPSQMNDAQMEAWD
                     SFSSRFARTSDIFLSKYVRAYILKDDPAFDGGFHDQLNRAAKLGLIENVEIWVDIRQL
                     RNATVHEYSDQDLEKIYEKFRRYSPLLIELRKKLIHET"
     gene            complement(7941..9257)
                     /locus_tag="VIG33_18480"
     CDS             complement(7941..9257)
                     /locus_tag="VIG33_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011162807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY8272887.1"
                     /translation="MRKPTEDARVFAERRQRIGRDLQGSAIIVAAHPEQIRNHDVGYP
                     YRQDSNMYYLTGFEEPESILVFRPGLKPESVIFCRRRDKERETWDGFRYGPEGVEREF
                     KVDKAYPIDEFMIHAPGLLKEVDQIYYALYKNKEADKKIQTCLEQVKSMQGRTGYGLL
                     PIHDADTFLGEFRLVKSEIELTNLREACEISAQAHLAAMRFTRPGVNERQIQGVLAHN
                     FFMKGAAREGYNYIVASGNAATTLHYNFNDQVCNDGDLLLIDAGAEYNYFTGDITRTY
                     PVNGKFTDDQARVYQGVLDVQKKIIDYIKPGIVFKDLHDMGTSLLTDLMLELGLLSGR
                     KEDLISALQQKKYYPHGIGHWLGMDVHDAGLYYKKGIPREVEPNMCFTVEPGLYIPVD
                     DTSAPQKYRGIGIRIEDNVRVTSHGCENMTSSAPKEIADLEKVVGK"
     gene            complement(9293..>10031)
                     /locus_tag="VIG33_18485"
     CDS             complement(9293..>10031)
                     /locus_tag="VIG33_18485"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY8272888.1"
                     /translation="GGAKAYGHIGVLQNMSRLKIPIFATVGIEWGAPVAALLSSKGQV
                     YDVEWQMFKLKDNELMKKSLIGSSAKSNEVPALKEFFQAAFGRQRVEDFHHPFGCPAY
                     NIAKNQVYMMSRGQVEQLMPYCLPYPPLFKPFNRNVSAVRELKMAADYLRSQGANYIV
                     FVNVLGRDAGRKSLVRESETNSAESVTWSEIASFYAKPQAGIDAMIPVELDSYMVTDF
                     EKRREIMQKGAEVSQKPIENWARKLGF"
BASE COUNT         2798 a         2396 c         2018 g         2819 t
ORIGIN      
        1 acgattttta atttctcccg ctagcagatt cagggtggtg tctaaggatt cgtgaaggtc
       61 gatctccttg agcttggcct cttccaagcg cgaaaaattg cgaagtccca aaacaatgtc
      121 cttggttcga cgtgcaccat cttcacagga agcaatgagc ttgggcatat cttgaacaat
      181 gtaatcatat tcgaaatcgg ctttgagggc cggaatattt tcaggatctt tctcggccat
      241 ctcgacgagc ttaataagtt tttcagagta atcccgcaaa tgagtcatgt tgcttgaaat
      301 gaaaccaatc ggattgttca actcgtgagc gaccccagcc accaactgac cgagactgat
      361 cattttggct gagtgaacca gtttggcctg agtgtctttg agctcctggt tcgccgcttc
      421 taactcgaaa attttctttt tcagctcagt cctggcttgt gaaatgcgcc tgctcatttc
      481 attgaagctc gctgtcagca ggccaatctc tgtttcattc tttaccggaa tttcgacgga
      541 attttctgaa gattcaaatc tttgtaaagc ccccaccaat tcatagagag gttttaaaac
      601 ccaattgctg gtcaccagaa tggtgataaa aagaagaatg acgattgcca ccactacaga
      661 gataaaagcg aaattcacct tccgaagaac ttccttggcc tcactcttag aggcgcctaa
      721 tcccacatag agctttcccg aatcccaatc caaaggagaa attaagaatc catagggcat
      781 agaacgaata ttcaaatcaa aaaagggctc tttatttggc ttgaaataag gttcaaaaaa
      841 gtctttcttg tagagataaa aatccggatg actggccatc atcagttggc cagtctcttt
      901 gaagaaaaga acctctaatt taagccgtgc cttcaagcga tttaaaaagc ctttatctag
      961 atcaatgatc tgctcaaagt atcccacgat cttacccgcg ccattggtta ctcgagagat
     1021 cagaagtaag gaagttttat gttgctccgt aaactcgaca gagccgactt ccttttgatt
     1081 cttaaaggtt gaaatatatt tcgcgttgag aaaaaccgct tgagaagagg gtgaaaaatt
     1141 tctgatctcg ccattttcat ctctgaaaac actgacaatc attctgccat cacgggcaaa
     1201 gaaggtcagg cttgacgtaa agtcttgctt caaccattga ctggcaatac cccggatggt
     1261 ggccccatca ccaatggaga gatgataaat caaatttgga tcacggatgt acttatcccg
     1321 tttttgtcga agaccagcac gatattcact aaagatcaca ctgatttcgc gggcatttcc
     1381 tgataagcgc tgagaaagct cacgatccaa agccttttca tacttgatca ttgagtaaac
     1441 agtaacgaac gccaacggaa tgactgaaaa tagcaagaac caaacgatca agattgttct
     1501 aagcgatctt ttaggctttc tttttaactg ctgcacagag gtgttccctg gtttactttt
     1561 tagcttcctc aacactgata atgagacgaa taattccatc aggcattact cgcggatgct
     1621 tatccttgct tattttagcc tttccatact cagagaggaa ggcctgtaat tcactgtatt
     1681 tagcttcggg aatagtgaaa tggaaataag cgaatcctgg agttttttgc cagccgagct
     1741 cgacctcacc agccttacgt ccaccaagtt ctatgatctt ctctttgatc tttggcccca
     1801 cagcctcgag gtttgtgact gcaatttgac ctcggtataa ataacccccc gttgtcttga
     1861 tgctttcctc actattttca tcagattctt cttcttcagc tttctctgta acttttgcta
     1921 cgacactgcc ttgagccttg ggtatcggaa gaggcttcgg agttgctttc tccactgggg
     1981 cgagaggagc cgctggtgga acagcaacgg actctttgtt attagccttt atgccttcgt
     2041 cgacaaattg cggagcttca gctgcttgtc ctgtagctgc tgctacctga agatctttct
     2101 tagcttctga agatttatta acttctgtca ggaccacatc atttgtgtcc atcgagagac
     2161 gcagcttcat gattttttcc cagggagcca ccgtcaagaa agtcacaatg acgaaaacga
     2221 ctacgagagc ttcaagtccc cacttcaatc ctacaggcca ttgatcgaag cgcgattttt
     2281 taaatagcac tgataaatac gaagaaggag ctgccagtct ttgctctagg gccggagaga
     2341 ctgtgatttg tctcattctc tccgcatatt gaaggcccgt ctgaattttt tggaactcta
     2401 actgcgcatc ctttgaagct gcgagatata tttcaacagc ttttttgcgt tcttcatcca
     2461 acatgctgct catgtaatca taaagcagct cattccctgt gaagggagtc acttggcgtt
     2521 gacctttttt agaaagcggt acgcgattta aatcttcaga catactctca ctttaccgca
     2581 tttagtttct ttgtagaaac accttgagcc atggatccga gctttcggag ggcacgacct
     2641 agacgataac gaacggttcc ttcagaaata ccaagacctt gggcgatgct cttctctgaa
     2701 atttccaaaa ccttcgacca aatgacagct aaaagctcat cttgggtcgc tagcttttga
     2761 aactcgcgcc aaggccctat atccaaatct gccggaatca tccaccctgc atcagttgaa
     2821 acattgggat ttccccggtt cagcttcaaa tgatgtcttg tccaaacaga aaaagtcgac
     2881 ataactatca acacttcatt attgaactga gaatttttac tttttttatc tgaataaatg
     2941 gaaagggctt gagtggaagc ttccagagcc ctgcgatcat ctaaaaaagc aaagaagaaa
     3001 aagagggcta tagattttac ttcaggctcg cccattcatt tccttaatca atggtcaact
     3061 gtcgcaatag atccaattga tcgaggactt tccccgaccc cataactacg caacttaaag
     3121 gatcttcggc gatagaaact ggcaagccag ttctttctct cagaagaaca tccaaattag
     3181 caagcatcgc cccaccacct gtgagaacaa tcccattatc aactatgtca gaagccagct
     3241 ctggcggagt tttctctaac gctgtacgaa ccgcatcgac cacttcagat aaaggatcca
     3301 tcaaagcatc gttcacttga gaactggtga tctcgattgt tttcggagct cccgccacca
     3361 ggtcgcgacc tttgatctcc attgtttttt catcttcaaa aggataagca ttgccgattt
     3421 gaattttaat caattcagca gttctttctc caattaaaag attaaattgg cgacgaacat
     3481 agttaatgat ggcttcatca aacttatccc ccgcaacctt gatagactta caataaacaa
     3541 taccgcctaa agaaataacg gcgacaccag tggtaccgcc gcccatatca acgaccatgt
     3601 ttccagaagg ctctgtgatc ggaagaccgg cgccaatagc tgccgccata ggctcttcga
     3661 tcaaatatac ttcgcgagcc cccgccgact gagccgcttc ttttaccgca cgtttttcca
     3721 cctgagtaat tccataagga acacagatga taatacgcgg acgaataaaa gattttttct
     3781 cgcccatgga tttagcaatg aaatatttta acatcgactg tgtcacttca aagtcagcaa
     3841 taactccgtc tttgatcgga cggatggcaa cgatactgcc aggagttctt cccaacatat
     3901 cttttgcctc tttaccaaca gccagaactc gattttgcat tccacggtaa tttttttgta
     3961 cagcaacaac agagggttca tccaaaataa ttccgcgtcc tttgacatag accaaagtat
     4021 tcgcggtgcc taaatcgatc gcgatgtcgt tcgaaaagta atctgtgaat ttttcaataa
     4081 ttgacattat tctctcacca agaaattagg tttgacctga agtctagttt ccacaaaaat
     4141 gagagtcaat gcagatattt agactatggc cagagattac gtcacgttgt gttatgggtc
     4201 gactgtttaa agaaacgact tccaagcaag ttctctttcc atatactgaa gaagtgcttc
     4261 cttttgtctt tgcaattcag cctccggcca agccagtttc tctttgaaaa cttcgcaaat
     4321 actctctaat tgcggcattc catgatcagg gcgtgctaaa aataggggca ctcttctcgc
     4381 atagaaatcc actaaattca gacacaaagt gtgatcgatg gcttgagctg cttcgagttg
     4441 ccagtaattg tattgcatcg gataacggtg aaggatttcc gcagcttcca tgccataacg
     4501 ctcggccaac ttttgtgtaa tcacttcagg ccttccactt tcctgagccc agagagctga
     4561 catgcccatc gcctgctgaa aagaatccac attggtgtag ggattcaggg ctttcaaagt
     4621 atcattttta gaaaatcgaa ctttctcgtc aaaactaaaa aactttaagc tatggcaaac
     4681 aacttgctcg gacattaatc tgtaggttgt gtattttcca ccagccacta aggttacacc
     4741 acgctcatca tcaataatca catgctcacg gctggttttt ccctcggagg aagattgatc
     4801 tttcactagg gggcggactc ccgcataggt cgcaacaatg tccttttcag tgatggcagc
     4861 gccagggaaa tattgatggg tgacttccaa gagatacttc acatcatccg ctgtcgccac
     4921 aactccatca ggaagttccc ggtagtctgt atcggtggtt ccgatcacaa ccatttcgtg
     4981 tcgaggaatg ccgaacacga tgcgatcgct tttctcagcc gccatcacca ccgcgctgct
     5041 taacggcaaa cgagattttt gcaaagtgag atgaattccc ttcgttgggc gtaaaatatt
     5101 ttgccacccc ttcaaaagtt ttggaccaag atcatcagtc catggaccta cacagctcac
     5161 cacgtgttta gcgcggattt tgaatttttt agaatccaat tggtcttcgc attcaactgc
     5221 gtcaatcttg ccattcgctg cgaaactagc gcctacggct ttggcgaagt tggcgcaaat
     5281 cgcccccatc tcatgggctg agcgcaaagt ctcatgaact agacgatcat cgtccatgta
     5341 tgcatcagaa taaacatacg agcccgaaag cttatttttt tccaaggctg gcattcgcgt
     5401 caaagactct ttagcattca aacgttcatg catttctgga gcctgaaaaa gtgctaaggc
     5461 atcataaagc cacatgccca aacccatctt gaacataccg actcggctat tttcatagag
     5521 ggggaccatg aaacgcaaag gatgtaccaa atgaggagcc atttcaaata atcgagtgcg
     5581 ctcattcaag gcctcaaaaa ccagatgaaa ttctaagttt tccaagtaac gaatcccacc
     5641 atgaatcagt ttactggatc gagaagaact tcctgacgcg aagtctttgg cttcaatcaa
     5701 agcgacacgc attccgcggc tcgctgcatc ccgtgccact cctgcaccgt taataccacc
     5761 accgataata cataaatcaa aaatttcttc ttgcatcttc cggatgtttt cgaggcgttc
     5821 tctcttagaa aatcttttca tagtggttaa gcctttgtag ttttaataga catgacatcg
     5881 ggattccaaa tctcagcttt aaagctagaa atggaattat taattcctga aacgtttgga
     5941 aaaaacacct tctgaaaatg cttttcccgg gaaattcgat aagccagctc gcaaagctca
     6001 tcacgggctt gcaaagaggc atattgcaaa tgctcaaccc aaaggctttg agtttgaccg
     6061 gccaggaaca acaaaggttg agcgatatag tagccgacta aaagaccttc atctgaaaat
     6121 tcgctggttt tatctggatc acggactaag aaagaccaac ccaaaggaaa ataatgctcg
     6181 agggcttctt tgcgccaacg ggcattccaa ctatgcaagg tcctttccat ctcgtcggga
     6241 ataagctctt tgagcttttt attttcaaaa tcgatgattt cattcaggtc ttcgagctgg
     6301 atcactcgac aaaaaaggtt catttttccg aactctccta ttaacacttc ttgtcaattt
     6361 aatgatctta tttcatacta agacgctatg aaatacaaag aatattcaaa agaaatttgg
     6421 aatcgaatca atagtacact ggactatgct ctggcaaaag actcagagcc gattgcggct
     6481 tttgacgccg atggcactct ctgggacacc gacttgggag aatctttttt tcaataccaa
     6541 atcgatcaca aactgatttc cctaccggac tctccatggg aattttatca agagttaaaa
     6601 aagaaagatc caagtgaagc ttacctgtgg ttggcacaaa tcaatcatgg ccaaaagcta
     6661 gacgttgttc accagtgggc agaacgtgcc cttgaagcct ccgcacccgt tccccttttc
     6721 gaggaacaaa agaaaattgt agagcttttt ttaagtaaag gtgtgaaggt ttttattatc
     6781 actgcctctg tcaaatgggc cgtggagcct ggagcaaaaa ttctaggact gggacgcgat
     6841 tccgttatcg gcattgaaac tcagattgaa aacgatctga tcactcaaaa acaaaaaggc
     6901 atcattactt atcaaatggg aaaggtcgaa gcccttctcg agcgcaccaa tggtaaaaag
     6961 ccattctttg cctctggcaa cacgatgggc gatttccagc ttttgaactc agcctctggc
     7021 ctcgccttgg cggtcagtgc cgcgaatcaa gacgatcgat tgtttagagc cgagaatgat
     7081 ctgcaagcaa ttgctgagca aaaagcttgg gtacgacata gatttatcta gacgacaggg
     7141 ctaagaggga ggccccttgc ttctctcttt gatctattac ttaaaactgc ctctcccaga
     7201 aagagggatg gctcctctta aaatagatcg taaaaactca tctgaagcac actcttgctc
     7261 aatagccaca gtgatatcaa ttcgctgctc agacagagtt tcgaatatct taatccgaat
     7321 tttatttttt agctctacca cagtttcttt ttcactggca gggacgagga gcaaaagatc
     7381 aatatctcca cctttctttt tatcatctgc acgacttcca aaaagataaa gtttaaaagg
     7441 aatttcctga agacacgctc cgatcgcatt tttgatgcct tcttgttctt taaaactaag
     7501 tctcatgaat tagtttcttt ctaagctcaa taagcaaggg agaatagcga cggaattttt
     7561 cataaatttt ttcaagatcc tgatcactgt attcatgaac cgtggcattt cttaactgac
     7621 ggatatcaac ccagatttct acattctcaa taagtcccaa ctttgctgct cgattgagct
     7681 gatcgtgaaa accaccatcg aaagcgggat catctttaag aatataagca cgaacatatt
     7741 tgctaagaaa tatgtctgat gtgcgggcaa atctagaaga aaaactatcc caagcttcca
     7801 tttgcgcgtc gttcatttgc gagggattta atggcattgt aagagtacgc tggtagctgt
     7861 actccaagta tttgagcgac ttgacgatct tagaatagta aagaccagtt aaatccatat
     7921 ttccctgcct cacaatgctc tcattttccc acgacttttt cgagatccgc aatttccttg
     7981 ggcgcagaag aagtcatgtt ttcacagcca tgggaagtga ctcgcacatt gtcctctata
     8041 cgaatgccga tgccccggta tttttgaggc gcgctcgtat catccacagg aatgtagagg
     8101 cccggttcca cagtaaagca catgtttggc tctacttcgc gagggatgcc ttttttgtaa
     8161 taaagtcccg catcatgcac atccattcct agccagtgac caatgccgtg ggggtaatat
     8221 tttttttgct gaagcgctga aatcaaatct tctttgcggc ctgaaagcaa accaagttcc
     8281 aacatcaaat ctgtgagtaa agacgttccc atgtcatgca gatctttgaa aacaatgcct
     8341 ggttttatat aatcgatgat ttttttctgt acatctaaga ccccttgata gactcgagct
     8401 tgatcatcgg tgaatttacc gttgaccgga taagtgcgag tgatgtcacc cgtgaaataa
     8461 ttgtactcgg ctccggcatc aatcaacagc agatcgccat cattacagac ttgatcatta
     8521 aagttgtagt gtaaagtcgt cgctgcattt ccgctggcga cgatataatt ataaccttcg
     8581 cgagctgcgc ccttcatgaa aaagttatgg gccaagactc cttgaatttg ccgttcattc
     8641 acacctggcc tggtgaaacg catcgctgct aagtgggctt gagctgaaat ttcgcaggct
     8701 tcacgtaaat tggtaagttc gatttcggat tttaccaagc ggaactcacc caagaaagta
     8761 tcagcatcgt gaatgggcaa tagaccgtaa ccggtgcgcc cctgcattga ctttacttgc
     8821 tctaagcaag tctgaatttt tttatccgcc tctttgtttt tgtaaagcgc gtaataaatt
     8881 tgatctactt ccttcagaag gccgggagcg tgaatcatga attcatcaat aggataggct
     8941 ttatcaacct tgaactcccg ctcaacgcct tcggggccgt aacgaaagcc atcccacgtt
     9001 tcgcgttctt tatcacgacg acggcagaag atcacacttt ctggtttgag gcccggacgg
     9061 aaaactaaaa tgctttccgg ttcttcaaaa cccgttaaat aatacatatt ggaatcttgg
     9121 cgataaggat atcccacatc gtgattacga atttgctccg gatgagcggc aacgataatc
     9181 gccgaaccct gaaggtcccg gccaattctt tgtctgcgtt cagcaaaaac tcttgcgtct
     9241 tcggtaggct ttctcataac tcttcctttg tcatcatgtc atccaatgca atctaaaatc
     9301 ctaacttacg ggcccagttc tcgataggtt tttgagagac ctctgctccc ttttgcataa
     9361 tctctcttcg tttttcaaaa tcagtcacca tataagaatc caactccaca gggatcatgg
     9421 cgtcgattcc cgcttgaggc ttcgcataga agctcgcgat ctcagaccac gtcactgatt
     9481 ctgcgctgtt agtctcagat tctctaacta aagattttct gccagcatca cggccaagta
     9541 cattcacaaa aacgatataa ttagcacctt ggctacgtaa ataatccgcc gccatcttta
     9601 gctctcgcac ggcgctgacg ttacggttaa acggtttaaa caagggtgga taaggcaaac
     9661 aataaggcat caattgctca acttgtcccc ggctcatcat ataaacttgg ttcttagcga
     9721 tattgtatgc tgggcatcca aaaggatgat ggaaatcctc aacccgttgg cggccaaaag
     9781 ccgcctgaaa aaattcttta agcgctggga cttcattgga cttggcgctc gagccgataa
     9841 gtgacttctt catcaattca ttgtctttta atttaaacat ctgccattca acatcataga
     9901 cttgcccctt ggaagacaag agggcagcga ctggagctcc ccattcaata ccgacagtgg
     9961 caaaaatggg gatttttaga cggctcatgt tttgaagaac tccgatatgg ccataagctt
    10021 tggctccgcc a
//
LOCUS       DATBTE010000490         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_1775993, whole genome shotgun sequence.
ACCESSION   DATBTE010000490 DATBTE010000000
VERSION     DATBTE010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775993"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="VIG33_18490"
     CDS             complement(<1..526)
                     /locus_tag="VIG33_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY8272889.1"
                     /translation="MFSLFRGKKAAQYIFFEMLPSFILGLIVFIFIILMFQVLRLTEF
                     ALVHGVDIKTIAEIVGYICISLLPALFPMSLLFAILLSYGRLSSDSEIVAMKASGLHM
                     GVLTTPAVVLALLVGILSAQTSFEIAPWGNRQFEVLFSRLGNTKAAATIKEGTFSEGF
                     FDLVVYANKVDSDKG"
     gene            616..1638
                     /locus_tag="VIG33_18495"
     CDS             616..1638
                     /locus_tag="VIG33_18495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272890.1"
                     /translation="MADMADNIIVSNKILLVYEEYSEMIGAQGILQKVGFDVLGISNE
                     YTLSENVLSFNPDLVVAFGRAGKVTTIGVGRRLREMTRWQGKVILVLPAGYKPNPQDF
                     AKIRADMLLEAPLPALRLMQIIGKMLGHDEALLQERLAKHGTETSKLEGVGVAGTGAD
                     ADRDDTLIVTGSAETVENFTVSGSATLNLNKEEKEKSKFGFDMASETSMPLKNEQNLE
                     ALWNELTENNELNITPEEDLAAKNRKGKKVSFDLEERNQEFSENELERLARSLQSAHN
                     TEAERVSKYVKFTAKITDFDSRQSLNKIASRKVQKEIASHWNLKEIEDQDDLRRGFAK
                     ALFRKK"
     gene            1707..2033
                     /gene="trxA"
                     /locus_tag="VIG33_18500"
     CDS             1707..2033
                     /gene="trxA"
                     /locus_tag="VIG33_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEY8272891.1"
                     /translation="MAIFTKPVTDSSFETDVLNSNTPVLVDFWAEWCGPCRALGPKLE
                     EVAQELNSKVKIVKLNVDENPGTPGKYGIRGIPAMLLFKGGKEVGQLVGNHPKDAIVD
                     FLNKNS"
     gene            2089..2604
                     /locus_tag="VIG33_18505"
     CDS             2089..2604
                     /locus_tag="VIG33_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272892.1"
                     /translation="MEQIAFQTIQKSIDNICGITAEEELDAISQALFDVQPALAGFVV
                     EFIEDMSEEAKDLGFMMALILWKSFEEKYPNLRTITEDEVVAKFEEQEVDLERLLQLN
                     EEILEEIQKNEMLNGQPEVLNYVIQELFAADEEEVTLDEDEELHLFMVLKFFSNCLND
                     VAKEVTESVKH"
BASE COUNT          872 a          507 c          639 g          735 t
ORIGIN      
        1 gtcccttatc agaatcgacc ttattcgcat agacaaccaa atcaaagaaa ccttccgaaa
       61 aagtgccctc tttaatagtg gcagcggctt tggtattgcc taagcgagaa aataaaactt
      121 caaattgacg gttaccccaa ggagcaattt cgaaagaggt ttgggcggaa agaattccca
      181 ccagaagagc caatacaacc gcaggggtag tgaggactcc catgtgaagg cccgaggctt
      241 tcatcgctac aatctccgag tcactgctca ggcggccata gcttaagaga atggcaaaga
      301 gcaggctcat agggaaaagg gcaggcaaga gagaaataca aatatatccc acaatttccg
      361 caatggtttt tatatccact ccatgcacta aagcgaattc cgtgaggcga agaacttgga
      421 acataagaat aataaaaata aaaacaatca gtcccaaaat gaagctggga agcatttcaa
      481 agaaaatata ctgtgctgct tttttaccgc gaaatagact gaacataggc tcctagttta
      541 aacgaaggga tatccgaaag ctttatttag ttgcaggaat tgcggccatt gtccagagga
      601 agctctcttg acccaatggc tgacatggct gacaatatca ttgtgagtaa caagatcttg
      661 ttagtttacg aagaatattc tgaaatgatt ggcgctcaag gcatcttgca aaaagtgggc
      721 tttgacgtcc tgggtatttc caatgaatac actttgagtg aaaatgttct ttcttttaac
      781 cctgaccttg tagtggcctt tggtcgcgcc ggaaaggtta cgacgatcgg tgttggtcgg
      841 cgcttgcgtg agatgactcg ctggcagggc aaagtgattc ttgtattacc ggcggggtat
      901 aaacccaatc ctcaagattt cgccaaaatc agagccgata tgttactgga agctcctctt
      961 ccggcattac gtttgatgca aatcatcggg aagatgttgg gccatgacga ggctcttctg
     1021 caagagcgtc tcgccaagca tggcacagaa acaagcaaat tagagggagt gggcgttgcc
     1081 gggactggag cggacgcgga cagggatgac accttgatag tcacgggatc ggcggaaact
     1141 gttgaaaact ttacggtttc aggttctgca acgcttaatt tgaataaaga ggagaaagaa
     1201 aaatccaagt tcggtttcga tatggcatcg gaaacttcga tgccattgaa aaatgagcaa
     1261 aatctggagg ctttatggaa tgagctgacg gaaaataatg aacttaatat caccccagag
     1321 gaagatctgg cggcaaagaa cagaaaaggt aaaaaagtaa gttttgatct tgaagaacgc
     1381 aatcaggagt ttagcgaaaa tgaactcgag aggctcgctc gcagcctgca aagtgcgcac
     1441 aatacagaag ctgagcgagt ctcaaaatat gttaaattta ccgccaaaat tacagacttt
     1501 gattctcgtc aaagtctcaa taaaatagcc agtcgtaagg ttcaaaaaga gatcgcaagt
     1561 cattggaatc tcaaggaaat agaagatcag gatgacctcc gtcgaggttt cgccaaagcg
     1621 ttatttagaa aaaaataatt gatccaatat gtattttcaa caatcatgtc cactttatga
     1681 tataggttgc aaatgaggag aagatcatgg ctatatttac taaaccggtg actgacagtt
     1741 ctttcgaaac ggatgtctta aattccaata caccagtttt ggtagatttt tgggctgaat
     1801 ggtgcggtcc ttgtcgggcg ctaggaccaa agttggaaga agtggctcaa gaattgaatt
     1861 caaaagttaa aatcgtaaag ttgaatgtgg acgaaaatcc aggcacccca ggaaaatatg
     1921 ggattcgcgg tattcctgca atgttgcttt tcaaaggtgg caaagaagta ggtcaactgg
     1981 tcggaaatca tccgaaagac gccatcgtag acttcttaaa taaaaacagt taagtggttt
     2041 ggtcctcaga cattttcctt tccctttaaa tcaaggagtt ttgctattat ggaacaaatc
     2101 gcttttcaaa ctattcaaaa atcgattgat aatatttgtg gaattacggc cgaagaagaa
     2161 ttggatgcca tctctcaggc tctttttgat gtacagcctg cattggccgg gttcgttgtt
     2221 gaatttatcg aagacatgag tgaagaagcg aaagatttag gattcatgat ggctttgatt
     2281 ctttggaaaa gtttcgaaga aaaatatcca aacttacgaa cgatcactga agatgaagtt
     2341 gtggctaagt tcgaagagca agaagtggat ctcgaaaggc ttttgcaatt gaatgaagag
     2401 atacttgagg aaatccaaaa aaatgaaatg ttgaacggtc agcctgaagt tttaaattat
     2461 gtaattcaag agcttttcgc cgccgacgaa gaagaagtta ctttggatga agatgaagaa
     2521 ttgcatttat ttatggtgtt gaaattcttc tctaactgtc tgaatgacgt tgctaaagaa
     2581 gttacagaaa gtgttaagca ttaatcttta gtattccttc gattggccag cctggccaat
     2641 cgtattattt cgagacacaa tttctattac atatatttct gaggttatgc cctctcttat
     2701 cttggatata aatacacgcc tgttcttact gattgttttt tttggttgaa tcc
//
LOCUS       DATBTE010000491        11568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Pseudobdellovibrionaceae bacterium isolate SMAG_U4175
            k141_545825, whole genome shotgun sequence.
ACCESSION   DATBTE010000491 DATBTE010000000
VERSION     DATBTE010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959073
            Sequence Read Archive: SRR11673482
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudobdellovibrionaceae bacterium (soil metagenome)
  ORGANISM  Pseudobdellovibrionaceae bacterium
            Bacteria; Bdellovibrionota; Bdellovibrionia; Bdellovibrionales;
            Pseudobdellovibrionaceae.
REFERENCE   1  (bases 1 to 11568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/14/2024 11:20:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,712
            CDSs (total)                      :: 3,668
            Genes (coding)                    :: 3,660
            CDSs (with protein)               :: 3,660
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 2 of 8
            Pseudo Genes (incomplete)         :: 6 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11568
                     /organism="Pseudobdellovibrionaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545825"
                     /isolate="SMAG_U4175"
                     /isolation_source="soil"
                     /db_xref="taxon:2026715"
                     /environmental_sample
                     /country="United Kingdom: Aberdeen, Scotland"
                     /lat_lon="57.1863 N 2.2145 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /gene="pilO"
                     /locus_tag="VIG33_18510"
     CDS             <1..327
                     /gene="pilO"
                     /locus_tag="VIG33_18510"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEY8272893.1"
                     /translation="KSSEMIRGIDSVAKMAGVSIKMKKPGTVNKKEVVEDLPVDVTLE
                     GSYAQIAQFIYFTSTLERLTRVLNFSVSAQDDSKDKPLRFDGQVISYKLAPEPEKSDE
                     SEGQPK"
     gene            393..1106
                     /locus_tag="VIG33_18515"
     CDS             393..1106
                     /locus_tag="VIG33_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEY8272894.1"
                     /translation="MSLRFMTPGHTQMDTPPLPSNDVQKVEAPSSGDLPPEFSQDDQG
                     GTLPNEAKIPPPPSQPPMPPQRPEEPVSPSQSSGISPDSPAVVTTPPPTMEANNPAAA
                     MQTEGTAPVIPQFASDDQYTYDPTGHRDPFKPYKSYRPAPTSNNSSPVKVIDPMDPLQ
                     RWDVDRFSVVAILWEVRLPKAMVKDPDGKMYMISKSTKIGRNSGQVVAIREGEVVVVE
                     AIENEGVQTKEVKILELKK"
     gene            1133..3430
                     /gene="pilQ"
                     /locus_tag="VIG33_18520"
     CDS             1133..3430
                     /gene="pilQ"
                     /locus_tag="VIG33_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEY8272895.1"
                     /translation="MKGFIRLIIIGALISSLLSCASQQTEEEDSLTTSSYANNNSADS
                     ASSSDDFSAFDSADSTSSAENSDGVAEKTTPPQSLSKDSDLAIEDEMNQADGSDKQSA
                     DNKQFAQDDLDKEAAKELQPQELAEQAPPDQAPPVQVDDLPVAQESPPPIIEEPAPPA
                     NEPPVPAIVNEPSPAPVEAPAPEMASSEKLIEITGLKYKANDSGGTVIVEANGMVNYT
                     TRVNQDLKQYIVEIPNTILPKKLKRTLNTKDIKGAVGAVDAYQSPGSKTSRIVVQMRD
                     GFGEPIVQQEGNTLLIVTSPNGEADVATREEGNEKVHGEETLTKGEADVNVDMNNSKI
                     LASQSLEEFLAGNTKFYGRKISIETSNIDVRDALKFITEESGVNMVITDEVKGNISLK
                     LRQVPWDQALVVIMKARRLGYRRQGSVLRIVPVEELKKEEDDATKLVLARKNIEPLKV
                     RAFNISYAKVDELEKKIKDFLSERGKVVSDVRTSTIVVTDGEESLNRVMKLIQGLDSA
                     PPQVLIEGKIVEALDSFQRTVGVQWGFNGADVQIGNGTNGPVNLHPNLGVGQAATSPG
                     GLGFGLNVGTLDIFGDLTASLSLFEKEEKIKVLSSPRIVTLSNEKADISQTNELPVKT
                     LTPQPSGPPIATIQFKPLTLKLEVTPQVTGEGTVIMKVTVNRQIKGAGVGTADDSFSV
                     NSREANTRVIVKNGQTAVIGGIYRSDATEGVVGVPWLKDIPVLGHLFKGTTTDKQRSE
                     LLIFLTPRILAQAEGAGVHNSNSGE"
     gene            3435..4022
                     /locus_tag="VIG33_18525"
     CDS             3435..4022
                     /locus_tag="VIG33_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272896.1"
                     /translation="MKITKVFIYIGLGLSLLSCGKAKESELIVEITGNNAYLINASAQ
                     SCSNKVAVALSPATASPASDISPHYYTYQGATLSWKNTTDTAYIIQAKIEFNNNSNVT
                     KPCTIAGDELLSMFFDFDNNQAWDGSLAPATSVSVPRTKNSTCKIRCGGVPIADENKS
                     FSAIGTMTWTGFQRNPDTGEEKPIKATTSVKLTYQ"
     gene            4061..5389
                     /locus_tag="VIG33_18530"
     CDS             4061..5389
                     /locus_tag="VIG33_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY8272897.1"
                     /translation="MDLFSASQFPTSAPLSEVLRPKNLDEIIGQEKTVGAHSKLGQML
                     RKGLVPSLIVWGPPGTGKTTFALALAQHIQAHFVNLNAVDSGAKILREVGEAGRDRRL
                     QFNQKTVLFVDEIHRFNKGQQDVLLPFVEKGDLILIGATTENPSYELNKALLSRSRVV
                     VFERLTTEQLQIILDKVCRFYGVPIDQVLQPDSHKNLLELSDGDARRLINSLEIIFDY
                     YRFEKSPDSSVTQSSFPLDLEALRELLQMSPLGYDKSSEMHYDVISAFIKSVRGSDPD
                     AAVYYLARMLEGGEDPIFIARRLIILASEDIGNADPRGLSMAVSGLQAVEAIGLPEAG
                     ITLSQVTTYLASCPKSNRSYMALNQAKECVRETKTLPIPLHLRSSKTSLSKDFGYGQD
                     YKYPHSFENAWIEQAYLPQALENRIFYEPSTRGFEKTLKEYLQWLKKQKG"
     gene            complement(5476..5781)
                     /locus_tag="VIG33_18535"
     CDS             complement(5476..5781)
                     /locus_tag="VIG33_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY8272898.1"
                     /translation="MLITRWQAPLTPNKTQATMILESEGLEPYEETYAPQMKVHEHRH
                     PFCEVRLIIEGELLFNISGNQFLLRRGDRVEVPANTKHFHTAQGSATCVCICAQRAI"
     gene            6006..6524
                     /locus_tag="VIG33_18540"
     CDS             6006..6524
                     /locus_tag="VIG33_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015090011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY8272899.1"
                     /translation="MSSSNKHKSTATDLDSILNYVDSSRGLKAKVGEAGKIQIYQGLD
                     GKVFNFMSQDISEILHRTDVDGKPFIQLNLKGSHKVLFTDTLIGFKPVETQGLDISRI
                     PKVVTTPDLLSVFEAIEESLGAETGGDNEIEILKKVYFAILAGGEQVGFDLSFERKWL
                     NRLIASKSKASA"
     gene            6689..6764
                     /locus_tag="VIG33_18545"
     tRNA            6689..6764
                     /locus_tag="VIG33_18545"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6722..6724,aa:Ala,seq:tgc)
     gene            7038..8551
                     /locus_tag="VIG33_18550"
     rRNA            7038..8551
                     /locus_tag="VIG33_18550"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            8671..8747
                     /locus_tag="VIG33_18555"
     tRNA            8671..8747
                     /locus_tag="VIG33_18555"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8705..8707,aa:Ile,seq:gat)
     gene            8837..>11568
                     /locus_tag="VIG33_18560"
     rRNA            8837..>11568
                     /locus_tag="VIG33_18560"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         3306 a         2446 c         2965 g         2851 t
ORIGIN      
        1 aaatcttctg aaatgatcag gggaattgac tctgtagcta agatggccgg cgtaagtata
       61 aaaatgaaaa agccgggaac tgtaaataaa aaagaggtgg tggaagatct gcctgtggat
      121 gtcacgcttg agggaagtta cgctcaaatc gcacaattca tttatttcac ctcgacctta
      181 gagcgattaa cgagggttct aaacttctca gtgtcggctc aagacgactc caaagacaaa
      241 ccgctgcgtt ttgatggaca agttataagt tataaacttg caccggaacc tgaaaaatct
      301 gatgaatctg aggggcaacc gaagtgaagc taacgcgttg ggtgattacc tacattataa
      361 tggcttcaat agggttgtgg gtggcctttt tcatgagttt acgattcatg accccaggtc
      421 atactcagat ggatactccg ccccttcctt cgaatgacgt ccagaaagtg gaagctcctt
      481 cttcaggaga tttacctcca gaattttcac aagatgacca aggcgggact ctgccaaatg
      541 aagctaaaat tccacctcct ccgagccaac cgccgatgcc acctcagagg ccagaagaac
      601 ctgtttctcc ttcgcaatcg tcaggaattt ctccggattc gcctgcagtg gttaccaccc
      661 ctcccccaac gatggaagca aataaccctg cagcggcaat gcaaacagaa ggtacagctc
      721 cggtgattcc gcaatttgct tcggatgatc agtacaccta tgacccaacg gggcacagag
      781 atccatttaa accttataag agctaccgtc cggcgccgac atccaataat tcctcaccag
      841 tgaaagttat tgatccaatg gatcctttgc aaagatggga tgtggatcga ttctcagtgg
      901 tggctatcct gtgggaagtc cgcttgccga aggcaatggt gaaggaccct gatggcaaaa
      961 tgtatatgat tagtaaaagt actaaaattg gacgtaacag tggtcaagtc gtcgccatcc
     1021 gagagggtga agtcgtcgtg gtcgaggcta tcgaaaatga aggcgttcaa actaaagaag
     1081 taaagatact cgaacttaag aagtaagttc gacacaaaat caggaggacc taatgaaagg
     1141 attcattaga ctgatcatca taggcgcttt aatttcttca ctgcttagct gtgcttcaca
     1201 gcagacagaa gaggaggatt ctctcacgac ctcttcctac gctaataaca actcggctga
     1261 ctctgcttca tcttccgatg atttctcggc cttcgatagt gctgacagca ctagtagtgc
     1321 ggagaactcc gatggtgttg ccgagaaaac aactcctccc caatctttaa gcaaagattc
     1381 ggatcttgcc atagaggatg aaatgaacca agccgatggt tctgacaagc agtcggcaga
     1441 taataaacaa tttgctcaag atgacttgga caaagaggcg gcgaaggagc tccaacctca
     1501 ggagcttgcg gaacaagctc ctccagatca ggcaccccca gtgcaggtgg atgacctgcc
     1561 agtggcccaa gagtctcctc ctccaatcat cgaagagcct gctcctcccg cgaatgaacc
     1621 gccagttcct gctatagtta atgagccgtc gccagcgccg gtagaagcac cagctcctga
     1681 gatggcgtcg agcgaaaaac ttatagaaat caccggcctt aaatataaag ctaatgatag
     1741 cggtggcaca gtgatcgttg aagcgaacgg aatggtgaat tacacgaccc gtgtgaatca
     1801 ggatcttaag caatatatcg tcgaaattcc taatacgatt ttaccaaaaa aacttaaaag
     1861 aactttgaat accaaagata tcaaaggagc tgtgggagca gtggatgctt atcaaagccc
     1921 tggctcaaaa acctctcgga tcgtagtgca aatgcgtgac ggtttcggcg agcctatagt
     1981 tcagcaggaa ggaaatactt tacttattgt gacctcccca aatggcgaag cggacgtagc
     2041 tactcgcgaa gaagggaatg agaaggtcca tggcgaagag accctgacta agggtgaggc
     2101 ggacgtcaat gtggatatga ataatagtaa gattctcgcc agtcagtctt tggaagaatt
     2161 ccttgctgga aacaccaaat tctacggtcg taagatctcc atcgaaacaa gtaatataga
     2221 tgttcgagac gccttgaagt ttattactga agagagtggt gtaaacatgg tcatcaccga
     2281 tgaagtgaag ggaaacatca gtctaaaact tcgtcaggtt ccctgggacc aagcccttgt
     2341 ggtaattatg aaagcgcgca gactaggcta ccgtcgccaa ggtagtgtgc ttaggattgt
     2401 tcctgtggaa gaattgaaga aggaagaaga cgacgccaca aaattggttt tagctcgtaa
     2461 gaacatcgaa cctttaaaag tccgtgcctt taatatttct tatgcaaaag tggatgagct
     2521 tgagaaaaag ataaaagatt tccttagtga aagaggcaaa gtggtcagtg atgttcgcac
     2581 cagcactatt gtcgtgacgg atggggaaga gagcttaaat cgtgtcatga aattgatcca
     2641 gggtttggat tcagcgccac ctcaggtttt gatcgagggt aaaattgtcg aggctctgga
     2701 tagctttcaa agaacagtgg gggtgcaatg gggtttcaac ggagcggatg tgcaaattgg
     2761 caatggtaca aatggacctg tcaatcttca cccgaatctc ggtgtaggcc aggcggcgac
     2821 ttctcctggc ggtttagggt ttggtctcaa tgtggggacg cttgatattt ttggggattt
     2881 aaccgcaagt ttgtctcttt ttgagaaaga agaaaaaata aaagttctct cctcgcctcg
     2941 cattgtcact ctttccaatg aaaaggccga catcagtcag acaaatgagc tgcccgttaa
     3001 aactcttacg cctcagccga gcggtccgcc aatagcaacg attcaattta agccattaac
     3061 cctcaaacta gaagttactc ctcaggtaac gggtgaaggt acagttataa tgaaggtaac
     3121 tgttaaccga caaatcaaag gggctggggt gggcactgca gacgattctt tctcggtcaa
     3181 tagtcgagag gctaatactc gtgtcatagt gaagaacggt cagacagcgg ttattggagg
     3241 tatttaccgc tccgatgcga cagagggggt tgtgggtgtt ccctggttaa aagatattcc
     3301 agtgctggga catttattta aaggaacgac cactgataag cagcgttcag aattacttat
     3361 cttcctgact ccacgaatct tggcgcaagc cgagggtgca ggggtccaca attcaaattc
     3421 aggggagtaa gcctatgaaa attacaaaag tgtttattta tatcggatta ggactttccc
     3481 ttctcagttg tggaaaagca aaggaaagcg agcttatagt agaaataacc ggaaataatg
     3541 catatttaat aaatgcctca gctcagagtt gtagcaataa agtggccgtt gccttaagcc
     3601 cggcaacggc cagtccagct tcggatattt caccccatta ctatacttat caaggagcta
     3661 cactgagttg gaaaaacacc acggatacgg cctatattat ccaagccaaa attgagttta
     3721 acaacaattc aaatgtaact aagccatgta cgatcgcggg agatgagctt ttaagtatgt
     3781 ttttcgattt tgacaataac caagcttggg atggatcttt ggctcctgcg acaagtgtgt
     3841 cggtgccgag aacaaaaaat tcgacctgta aaatccgttg tggtggggtt cctatcgctg
     3901 acgagaataa gagcttctcc gcgattggca ccatgacatg gactggcttt caaagaaatc
     3961 ctgataccgg ggaagagaag cctataaaag ccacgacatc cgtaaagctt acctatcaat
     4021 agatttgcca ggcgggacct cataggctaa gcctgtccat atggatctct tttcagcctc
     4081 tcagtttccc acatcggctc ctttatcaga agttcttcgt ccgaagaatt tagatgaaat
     4141 tattggccaa gaaaaaaccg taggagccca ttccaaactc ggccaaatgc tacgcaaggg
     4201 attggttcct agcctcattg tttggggccc tccaggaact ggaaaaacca cttttgcctt
     4261 agctcttgcg cagcatatcc aggcccactt tgtgaacctc aatgccgtgg actccggcgc
     4321 gaagatcctg cgtgaagttg gcgaagcagg ccgtgaccga cgattgcaat tcaatcaaaa
     4381 aacagtgctc tttgtggatg agattcatcg cttcaacaaa ggacagcagg atgtgctcct
     4441 gcctttcgtg gaaaagggag atttgatcct catcggagcg acgacagaaa atccgagtta
     4501 tgaactcaac aaagcccttc tgagtcgcag tcgggtagtg gtctttgaaa gacttactac
     4561 tgagcaactc caaatcatcc tcgataaggt gtgccgtttc tatggcgtgc ctattgatca
     4621 agtattgcaa cccgattctc ataaaaactt attggaactc tccgacggtg atgcccgccg
     4681 cttgatcaac tctctcgaga ttatttttga ctattatcgt tttgaaaaga gcccagactc
     4741 ctcagtcact cagtcaagct tccccttaga tttggaagct ttgcgcgaac ttttgcagat
     4801 gagtccgttg ggttatgata agtcttcaga gatgcattac gacgttatct cagcgtttat
     4861 taaaagtgtc cgaggaagcg atcctgatgc tgccgtttat tacctagccc gtatgctgga
     4921 agggggagaa gaccccatct ttatcgcccg ccgtttaatt atcttggctt cggaggatat
     4981 aggtaatgcc gatccccgcg gcttatccat ggcggtgtct gggctgcaag cagtcgaagc
     5041 cataggtctt ccggaggccg gcatcacgct ttcgcaggtt acaacttatt tagcttcttg
     5101 tcccaagtcg aaccggtctt atatggcttt aaatcaagcc aaagagtgcg tgcgagagac
     5161 taagactttg cctattcctt tacatcttcg ttcttcaaaa acgagccttt cgaaagactt
     5221 tggttatggt caggattata agtaccccca tagttttgaa aatgcatgga tagaacaagc
     5281 ctatttacct caggcgctcg agaacagaat tttctacgag ccttccactc ggggattcga
     5341 aaaaacactc aaagaatatc tccagtggct taaaaaacaa aaaggctagg aaaaacttct
     5401 ttctattgcc cctatcttac ttaaagatga aaggggaaaa ttgagccttt ttgttttttt
     5461 aaattcgaaa cgcgtctaga tcgctcgctg cgcacaaatg cagacacagg ttgcagatcc
     5521 ttgagcggtg tggaaatgtt ttgtattagc aggaacctcc acgcggtctc ctcgacgtaa
     5581 aagaaactgg ttgccagaaa tgttaaaaag caattcgcct tcaataatta ggcggacttc
     5641 acaaaaggga tggcggtgtt cgtggacctt catttgagga gcataagttt cctcataggg
     5701 ttcaagtcct tccgactcta aaatcattgt tgcttgagtt ttattggggg taaggggcgc
     5761 ttgccagcgt gtgattaaca tatctgctcc aaatgacctt gatgtcatct tgatttcgag
     5821 atctcgtcat taacacccta aaggtctcaa aaataggctg ctcccacaag gctctctgcg
     5881 ttcagttttg agacgctagg cccagataca agaccggatt cgagagaaag cgaaaaaata
     5941 tataaagtcc cttaaaacga tcgacgatag agagaatgtt agagtgctat tcttaggagg
     6001 gttcgttgag tagctccaat aaacataagt cgaccgctac agatctggac agcatcctga
     6061 attatgtcga ttcatcacgt ggcttaaaag ctaaagtcgg tgaggctggg aaaattcaaa
     6121 tttatcaagg tctcgacggc aaagtcttta attttatgtc tcaggatata tcggaaattc
     6181 tccatcgcac agatgtggac ggaaagcctt ttattcaatt gaatttaaag ggcagtcaca
     6241 aagtcctatt tacggataca ttaattggct ttaaacctgt ggaaacacag gggttagata
     6301 tttcccgaat ccctaaggtc gtcaccactc cagatcttct tagtgttttt gaagctatcg
     6361 aagaatccct tggggccgaa acaggagggg ataacgagat tgagatcctt aaaaaagtct
     6421 attttgcgat cctagccggt ggagagcaag ttggatttga tctaagtttt gaaagaaagt
     6481 ggctcaatag acttatagcg tcaaaatcaa aagcgagtgc ctaattctct attaaatcta
     6541 ttcatttcag catcttaata gaaacagatt gttaaaatac aaacatctgt tttgtttcta
     6601 ctttaagcac acctactgct cgaaaatttt tttataaatt gctattgacc tttggatagg
     6661 tttgatagag attcgtcgaa ctttgaacgg gggcatagct cagctgggag agcatctgat
     6721 ttgcattcag aaggtcgcag gttcgatccc tgttgcctcc accaaccgct tcaaagcaga
     6781 taactttttt cttttttcga aattaaaatt tgaaaaaaga aaaaagatgt tgacgggcag
     6841 ggggtaaatg aataaaacca ttacctctcg caacgatgag aagaaaactt gttaagagaa
     6901 tttcgctctt tgaaaactaa atagctagat agaaaaattt tgggctctta gagtaagtag
     6961 caatacttac tcacaatttc aaaatattcg aacaaacaat agcgtaaaag ctagctgttt
     7021 gatcagaaac agaattataa ctggagagtt tgattctggc tcagaacaaa cgctggcggc
     7081 gtgcctaata catgcaagtc gaacgaggaa agtagcaata tgagtactag tggcgcacgg
     7141 gtgagtaatg cgtggataat ctgccttcaa gtgggggata actagtcgaa agattagcta
     7201 ataccgcata agaccacagg atctgaggat ctcggggtta aaggtttttc gcttgaagat
     7261 gagtccgcgt gagattagct agttggtgag gtaatggctc accaaggcaa cgatctctaa
     7321 ctggtctgag aggatgatca gtcacactgg aactgagaca cggtccagac tcctacggga
     7381 ggcagcagta gggaatattg cacaatggag gaaactctga tgcagcgacg ccgcgtgagt
     7441 gatgaaggcc ttcgggtcgt aaagctctgt cgcaggggaa taacacaatg aatgtaccct
     7501 gtaagaaagg atcggctaac ttcgtgccag cagccgcggt aagacgaggg atcctagcgt
     7561 tgttcggaat tattgggcgt aaagcgggtg taggtggctt tgtaagtcag gtgtgaaagc
     7621 ccagggctca accctggaag tgcatttgat actgcgaagc ttgagtgtag gagaggttac
     7681 tagaattctt ggtgtagtgg tgaaatacgt agatatcaag aggaataccg gaggcgaagg
     7741 cgggtaactg gcctaacact gacactcaga cccgaaagcg tggggatcaa acaggattag
     7801 ataccctggt agtccacgct gtaaacgatg gatacttgtt gttggaggta ttgacccctt
     7861 cagtgacgaa gctaacgcgt taagtatccc gcctggggag tacggtcgca agattaaaac
     7921 tcaaagaaat tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgatgcaac
     7981 gcgaagaacc ttacctaggc ttgacatgta ctggaagact ggcagaaatg tcgtcgctcg
     8041 caagagtcgg tacacaggtg ctgcatggct gtcgtcagct cgtgtcgtga gatgttgggt
     8101 taagtcccgc aacgagcgca acccctgcat ttagttgcca gcattcagtt gggcactcta
     8161 gatggactgc cggtgttaaa ccggaggaag gtggggatga cgtcaagtcc tcatggccct
     8221 tatgcctagg gctacacacg tgctacaatg gtaactacag acggaagcta agctgcaaag
     8281 tggtgcaaat cctacaaaag ttatctaagt tcggattggg gtctgcaact cgaccccatg
     8341 aaggtggaat cgctagtaat cgcggatcag aacgccgcgg tgaatacgtt cccgggcctt
     8401 gtacacaccg cccgtcacac catgaaagtt ggctgtacca gaagtcgctg cgctaaccgc
     8461 aaggaggcag gcgcccaagg tatggtcgat gattggggtg aagtcgtaac aaggtagccg
     8521 taggggaacc tgcggctgga tcacctcctt tctaaggaat ttctatcaac ttcggttgat
     8581 acattcttag gtcaacgcga atcttcggat ttagcgtgag tcccaaaatt ttttctagct
     8641 atttagtttt gaaagagtga aggcttaaaa gggcctgtag ctcagttggt tagagcacac
     8701 gcttgataag cgtggggtcg gaagttcaag tcttcccagg cccaccaaat gagccgctta
     8761 aaaagttttt agtattttag ttctttgaca attgaataga ttgatttagt tgatttttag
     8821 cgaggttaat taccatttta aagctacaaa gggcttacgg tggatgcctt ggcactaaga
     8881 agcgatgaag gacgtggtaa gctgcgataa gcttcgggga gcggcacaca cgctttgatc
     8941 cggagatttc cgaatggggc aacccaccaa ttattggtat cttgcagtca atacatagct
     9001 gcaagaggcg aacgagggga agtgaaacat ctcagtaccc tcaggaaaat aaatcaaata
     9061 cgagatttcc ctagtagtgg cgagcgaacg ggaaagagcc caaaccttat tcattaattt
     9121 gaataggggg ttgtgggacc gcgatgtgcg acttgagatt ctaggagaac agtctggaaa
     9181 gactggcaag atagggtgat agccccgtat ccgaaagagt taagagcgct agcggtatcc
     9241 cgagtaccac aggacacgtg aaatcctgtg ggaatctggg gggaccaccc tccaaggcta
     9301 aatattcctt agtgaccgat agaggacaag taccgtgagg gaaaggtgaa aagaaccccg
     9361 agaggggagt gaaatagaac ctgaaaccgt aagtctacaa gcagtggaag ctctttatat
     9421 gagcgaccgc gtaccttttg cataatgagt cagcgagtta tctttgcagg caaggttaag
     9481 ccgtgacagg tggagccgta gcgaaagcga gtctgaatag ggcgatacag tctgcaggga
     9541 tagacccgaa acccagtgat ctaatcatgg acaggttgaa gcgagggtaa cacctcgtgg
     9601 aggaccgaac cagttgaagt tgaaaattct ttggatgatc tgtgattagg ggtgaaaggc
     9661 caatcaaact gggtgatagc tggttctccc cgaaatatat ttaggtatag cttcaggtaa
     9721 attgtatcac ggaggtagag cactgaatgg gctaggggtc tttaccagat taccaaaccc
     9781 aaataaactc cgaatgccgt tgatatgtac cctggaaggc agacgctggg tgataaggtc
     9841 cagcgtcgag agggaaagag cccagaccac cagctaaggt cccaaaattt acactaagtg
     9901 gagaacgttg tggagttact ttgacaacta ggaggttggc ttagaagcag caatccttta
     9961 aagaaagcgt aatagctcac tagtctagtg actctgcgcg gaagatacaa cggggctaag
    10021 tgtagtaccg aagctgtggc tttatcgtaa gataaggggt aggggagcgt tctagcgtag
    10081 gatgaaggtt gaccggtagg acagctggac gaactagaag tgatcatgct gacataagta
    10141 gcgttgaact cgggtgaaaa acccgggcgc cgaaaactca aggattcctg ggcaaggata
    10201 atcctcccag ggttagtcgg gacctaagat gaggccaaat ggcgtagtcg atggcaaaac
    10261 ggttaatatt ccgttaccga tctcggattg ctataggaat gacagagtag gctatctcgg
    10321 cctgttattg gattcaggtg taagcgtgta ggtggtgtcg taggtaaatc cgcggcacat
    10381 aacactgagg cgtgaatgcg agggacttgt cccggaagcg agaaaagcca tgctttcaag
    10441 aaaagtttcg atagtttaag agatcgcccg taccgtaaac cgactcaggt gagtggggtg
    10501 aatatcctaa ggcgatgggg tgaaatttgg ttaaggaact cggcaactta acaccgtaac
    10561 ttcgggaaaa ggtgtgccca cgaatgtgta aggacttgct ccccaagctt ttgtgggtcg
    10621 cagagaaatg ggagtagcga ctgtttatca aaaacacagg catgtgcaaa gtctcaagac
    10681 gaagtataca tgctgacgcc tgcccggtgc tggaaggtta aaaggattag ttagcgcaag
    10741 cgaagctgag aattgaagcc ccagtaaacg gcggccgtaa ctataacggt cctaaggtag
    10801 cgaaattcct tgtcgggtaa gttccgacct gcacgaatgg cgtaacgact tctccggtgt
    10861 ctcaaccaaa tgcctcgcga aattgaattc tcggtgaaaa tgccgagtac ccgcagaaag
    10921 acggaaagac cccgtgaacc tttactgtag cttggcagtg attttagagt tttcatgtgt
    10981 aggataggtg ggagcctttg aagccagatc gctagatttg gtggaggcaa ccttgaaata
    11041 ccacccttga aaactttgac atctaacttg cgctcataat cagggcgaag gacactgtct
    11101 ggtgggcagt ttgactgggg cggtcgcctc ccaaaaagta acggaggcgc gcgatggtcc
    11161 cctcagcttg attggaaacc aagcgtcgag tgcattggca taagggggct tgactgcgag
    11221 acttacaagt cgagcaggtg cgaaagcagg ccaaagtgat ccggtggtcc cgcgtggaag
    11281 ggccatcgct caacggataa aaggtactcc ggggataaca ggcttattcc atccaagagt
    11341 ccatatcgac gatggagttt ggcacctcga tgtcggctca tcacatcctg gggctggagc
    11401 aggtcccaag ggtttagctg ttcgctaatt aaagtggtac gcgagctggg ttcagaacgt
    11461 cgtgagacag tttggtcctt atcttctgtg ggcgtatgag atttgagcag acctgtcctt
    11521 agtacgagag gaccgggatg gacgaacctc tggtgttcct gttgtcgc
//
